LOCUS       DACEEZ010000001       104842 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20313_2017
            SAMN12639966-rid9879223.denovo.001, whole genome shotgun sequence.
ACCESSION   DACEEZ010000001 DACEEZ010000000
VERSION     DACEEZ010000001.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639966
            Sequence Read Archive: SRR10023158
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 104842)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 104842)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741683.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741683.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:44:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,016
            CDSs (total)                      :: 2,962
            Genes (coding)                    :: 2,889
            CDSs (with protein)               :: 2,889
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 36 of 73
            Pseudo Genes (incomplete)         :: 29 of 73
            Pseudo Genes (internal stop)      :: 18 of 73
            Pseudo Genes (multiple problems)  :: 8 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..104842
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639966-rid9879223.denovo.001"
                     /isolate="E20313_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(227..781)
                     /locus_tag="IWO89_000001"
     CDS             complement(227..781)
                     /locus_tag="IWO89_000001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HAQ0454795.1"
                     /translation="MKLSTDWRKEIQTIPNLLSIFRIFLLPIYLYFVLRQSFYIAGAV
                     IVVSGLSDYLDGVIARRYNQVTDLGKVLDPFADKLTQLFLILSMAWYRPWLWLLFGLF
                     LIKEGFMFVAGLIGLSKNIKLSGAKWYGKVATAVIYVGMILLLLFPELPTLWVRVIFA
                     VITYGLLQSFVLYAVEYRKMFQRK"
     gene            complement(907..2835)
                     /locus_tag="IWO89_000002"
     CDS             complement(907..2835)
                     /locus_tag="IWO89_000002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease MutS2"
                     /protein_id="HAQ0454796.1"
                     /translation="MDNEMISQLQFDRIKKEIQARAIGNYSKKRISDMTVSTNLQTVL
                     DRQEETREARLILESSQHVPFMGLPRIDALTEQVKKGLILQPTDLIEYADFLRSSRMI
                     TKFFDKNQYQTPLLFAYSKHLPDLINVEELIDQKIKNNKVSDDASRNLRKVRKQLQLI
                     EKEIQSKLLKFLRHPKNKEMIQEAMIVQKGEYYTIPIKASYKNKIDGTIIDESNKRTT
                     VFIEPTVISKLNEHYQLLKAEEISEEYQVLAALTGAIAENEEVIDLLIETITVLDIIF
                     ARAKFSREINGSTPRINKSEHIIIKQGRHPFLPEHAVPLDVEIGKDYRGLIITGANAG
                     GKTVVLKTVGLLTLMAMFGMQVPAKEGTELAVFDEIFVDVGDHQNLENALSTFSGHMQ
                     NIAAILKKIKRNTLVLLDEIGSGTEPNEGAALAIAIMESMYEQGALIIATTHYGEIKK
                     FAGDHEDFVPAAMAFDREALRPKYQLRVGETGESQALWIAHKMDMSMKLIHQAERYLA
                     EKAYRTQKKEFLPKTAVTNRKENKKERQLFAKGDRIFVNEYQKEALVYEDIGEDTIDV
                     YLDKKIIHVPRQRVRLVRSAEDLYPTGYDLDSLFIDYKTRKRQRDLERGSKKAHKVLV
                     KEMRKRQEERRVNDENSK"
     gene            complement(3141..3857)
                     /locus_tag="IWO89_000003"
     CDS             complement(3141..3857)
                     /locus_tag="IWO89_000003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aquaporin family protein"
                     /protein_id="HAQ0454797.1"
                     /translation="MNSDMTQIMGEFIGTLILVLLGDGVCAAVNLNKSKAQASGWIVI
                     AFGWGLAVTMAVYISGFMGPAHLNPAVSLAMAMTGAISWNLVVPFIIAQVLGAFAGAI
                     LVWLSYLPHWNATKDESAILGTFATGPAIRNYPANVITELIGTFVLVLGLLAFGQNEF
                     APGTNVFAVGGLILAIGLSLGGPTGYAINPARDFGPRLAHAVLPIANKGTSDWAYSWV
                     PIAGPMIGAIIAVGVYSLMV"
     gene            complement(3858..5681)
                     /gene="glpO"
                     /locus_tag="IWO89_000004"
     CDS             complement(3858..5681)
                     /gene="glpO"
                     /locus_tag="IWO89_000004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glycerol-3-phosphate oxidase"
                     /protein_id="HAQ0454798.1"
                     /translation="MFSNKTRQDSIQKMQQEELDLLIIGGGITGAGVAVQSAASGIKT
                     GLIEMQDFAEGTSSRSTKLVHGGIRYLKTFDVEVVADTVGERAVVQSIAPHIPKPDPM
                     LLPIYEGEGATTFNMFSVKVAMDLYDKLANVTGTKYENYTLTPEEVLEREPFLKKEGL
                     KGAGVYLDFRNNDARLVIDNIKKAAEDGAYLVSKMKAVGFLYDGDQIVGVKARDLLTD
                     EVIEIKAKLVINTSGPWVDKVRNLNFTRPVSPKMRPTKGIHLVVDAKKLPVPQPTYFD
                     TGKQDGRMVFAIPRENKTYFGTTDTDYQGDFTDPKVTQEDVDYLLDVINHRYPEANIT
                     LNDIEASWAGLRPLLIGNSGSDYNGGDNGSISDKSFNKVVDTVSEYKENKVSRAEVED
                     VLNHLENSRDEKAPSTISRGSSLEREPDGLLTLSGGKITDYRKMAEGALRLIRQLLKE
                     EYGIETKEIDSKKYQISGGNFDPTKLEETVTELAKEGVAAGLEEEDATYIADFYGTNA
                     RRIFELAKEMAPYPGLSLAESARLRYGLEEEMVLAPGDYLIRRTNHLLFERDQLDEIK
                     QPVIDAIAGYFEWTEEEKAQQTKRLEALIAESDLRELKGEK"
     gene            complement(5695..7191)
                     /gene="glpK"
                     /locus_tag="IWO89_000005"
     CDS             complement(5695..7191)
                     /gene="glpK"
                     /locus_tag="IWO89_000005"
                     /EC_number="2.7.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol kinase GlpK"
                     /protein_id="HAQ0454799.1"
                     /translation="MTESTYIMAIDQGTTSSRAIIFDKKGRHIGSSQKEFTQYFPQES
                     WVEHDANEIWNSVQSVIAGAFIESGIKPNQIAGIGITNQRETTVIWEKDTGRPIYHAV
                     VWQSRQSAGIADNLKNEGYQEFFHEKTGLVIDAYFSATKIRWILDHVEGAQERAEKGE
                     LMFGTIDSWLVWKLTDGQAHVTDYSNASRTMLFNIHQLDWDQEILDLLNIPRVMLPKV
                     TSNSEVYGYTQGYHFYGSEAPISGMAGDQQAALFGQMAFEPGMVKNTYGTGSFIVMNT
                     GEKPQLSKNNLLTTIGYGINGKVYYALEGSIFVAGSSIQWLRDGLQMLQKASDSEDAA
                     KRSTSEDEVYVVPAFVGLGAPYWDQAARGAMFGLTRGTTKEDIIKATLQSIAYQVRDI
                     IDTMQDDTGIKIPVLKVDGGAANNEYLMQFQTDILNVPIQRAENLETTALGAAFLAGL
                     AVGYWKDTDEIREFYEAGKLFEVQMEEERREKLYNGWKKAVKATQAFE"
     gene            complement(7327..8787)
                     /locus_tag="IWO89_000006"
     CDS             complement(7327..8787)
                     /locus_tag="IWO89_000006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HAQ0454800.1"
                     /translation="MKIEALLERKEQLQILILRNLVLQGGTASINGLREHVQLSKASF
                     DQYLEDIELIGRMMEKKVEVQRNEYQALLVLDEDVSLEKILLFLLQESLKFKMLVYLL
                     EHQQVSIVRLATAFNISESSVFRKIKELNQLLEEFGLQIKNGQLYGEELQIRYFYYEL
                     FQYIPEDQRPLFLQNTPEKRPFILGLDRVLETTFTASAEAQIACWLGITKKRLLNEKS
                     TYATLKEKKLLYQSDRLYQAIDPIIVMHLSRTAAELNVYETMMFYSFFVSFSIVDEEI
                     FYQYDLTRSKKLPTAVLDTYIRETMLWHYRPRRLKIKEEKAVGYQISQINNEWFFFVG
                     KIEVYERDRLLEQQQKMLGHSLTQLLAKLQETAVQQLPRKRAEDSELSYLMIQYANVL
                     LMIDFYIAKPVVIGIDLESLPIYRIAFQQYLIRELRGIGGIEIGSYEEGKEYDLVITF
                     CQRNKKQCEYYLSEFASPYDIIRLKRRIETLKKEKN"
     gene            complement(8777..10072)
                     /locus_tag="IWO89_000007"
     CDS             complement(8777..10072)
                     /locus_tag="IWO89_000007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HAQ0454801.1"
                     /translation="MNIVIVGGSFAGIHAAIYLRQLMPTSEIYLIEKQSKLGWIPSGF
                     NLILKGKVTSEKQLSWITKEELTDRYRIHVYTEKTVIGLKEKKVVLAGGKKLDFDRLI
                     LATGSNQKFRNISAESTLIHPVKKIQNVTALQQKIMNAKKIAIIGAGQAGIEIAEGLA
                     SRKKQIHLYESRKSILFRYLDPEMTEPLVKEMKNQGLSLFLEEQVVSLTENETAIVET
                     EKRKENYDLVLLANHSRPDNQMWEEQLTLNDDGTIWVDDYLQTSQKDVYAIGDAIQVT
                     FRPTNEKMYVSLVNNAVRTARNVSKTISGSQTKDQGTYRPVGNQWFGYFLGSVGLTEE
                     ESIFYPQKIETGYVDYKLTAINQSKVKLKFLFDSEKKILGAQLQSKKEMFHLLDQLTI
                     AVEEGWTFEQLEEHELFFQPEYRVPDMVWSKVANTAYED"
     gene            complement(10404..11081)
                     /locus_tag="IWO89_000008"
     CDS             complement(10404..11081)
                     /locus_tag="IWO89_000008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glutamine amidotransferase
                     domain-containing protein"
                     /protein_id="HAQ0454802.1"
                     /translation="MKVLIVLTNVEKYEKIERPTGLWLSELTHFYDVLVKNGIDADFV
                     SPKGGYVPLEPQSVLNMDEIDWAYYEDEDFRNRALGNTLRPDDVDANDYAAVYYAGGH
                     GTMWDFPEAIDLGKVASQIYRNEGLVSAVCHGVVGLLPVTDESGKSILAGRTVTGFSN
                     EEEEANGTTNEVPFLAEDALKEKGANYQSGAAFSEVVQIDGRLLTGQNPQSAHQLGEA
                     VVEALKN"
     gene            complement(11111..12202)
                     /locus_tag="IWO89_000009"
     CDS             complement(11111..12202)
                     /locus_tag="IWO89_000009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-dependent flavin oxidoreductase"
                     /protein_id="HAQ0454803.1"
                     /translation="MSQFDQSITLPSGVQLKNRLMLSPMTTQLSFFNGVITEDEITYY
                     KQRSKGLGAVITGAANVQDIGKGWPGELSIAHDEMIPRLSELAKAIQGEGAKAIIQIF
                     HGGRMTSRATLSGEQPVSASAVAAKRPDAETPRAMSEEEIVETIQAFGKATRRAIESG
                     FDGIELHGANTYLIQQFFSPHSNRREDQWGGSLENRYRFIEELLTTVFSAVEQYAKKP
                     FIVGYRFSPEEYETPGIRFEDTIWLLERLRESKLDYLHVSLNMYDRIARSDKYNDKSI
                     LEVVHNTIQGKIPLVGVGGIRNREDVEQVLKYAELAAIGQQMIVDPDWAEKLLENRED
                     EFVTKPFEEAYKELYLPSPLYNFLKARYQ"
     gene            complement(12303..12737)
                     /locus_tag="IWO89_000010"
     CDS             complement(12303..12737)
                     /locus_tag="IWO89_000010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HAQ0454804.1"
                     /translation="MEKPIEKALDECLFFSVKKLDRMLNKLADEAFRRTGLAPTYGFI
                     LLILKEKDGIPQKDIAQMLYSAPSTIARFVEKLEYKGYVKTVSEGRLSLVYLTEQGRQ
                     FAKEVDGSWEELHQSYKAVLGNEASKELAQTMNDATDKLQKK"
     gene            complement(13321..14778)
                     /gene="ascB"
                     /locus_tag="IWO89_000011"
     CDS             complement(13321..14778)
                     /gene="ascB"
                     /locus_tag="IWO89_000011"
                     /EC_number="3.2.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HAQ0454805.1"
                     /translation="MKKFPEGFLWGGATAANQCEGAWDVDEKGLSVSDVYTFDSDLPK
                     EKWTDQWHMMTHQQVKAAQDPNSKYYYPKRHGNDFYHHFKEDIRLFAEMGFKCYRMSI
                     AWTRIFPHGDEETPNEAGLFFYDQVFDECLKYGIEPVVSLSHYEMPLYLVTEYGGWPN
                     RKLIQFYVRFAETVFKRYKNKVKYWMTFNEINCVKHHPFVSVGVIEENHPHLEQAKYQ
                     GAHHQFIASALATKACHEIIKGSQVGCMISYQLLVPYSCDPDDIQKTIEQQRTSLFFS
                     DVQARGYYPAYTQRMFEEKGVNLKIEVGDEEILAAYPVDFVSFSYYMSSAVSAHPENW
                     EGVQGNLITGGIKNPYLPSSEWGWQIDPKGLRIALNQLYDRYQKPLFIAENGLGAIDK
                     VEEDGTVQDDYRIDYLKQHIEQMKEAIKDGVDLFGYTSWGCIDIVSASTSQMSKRYGY
                     IYVDQDDEGNGTKKRLKKKSFDWYRQVIQSNGEIL"
     gene            complement(14775..16670)
                     /locus_tag="IWO89_000012"
     CDS             complement(14775..16670)
                     /locus_tag="IWO89_000012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAQ0454806.1"
                     /translation="MEKPFSKNYTYLVRRIATALINKGEVKMDYSQLAKDIVRFVGGE
                     ENVSNVYHCATRLRFTLKDNKKADKEKVEQLEGVITVVEAGGMFQVVVGNAVNEVYDV
                     LSKQMKLEDDASSGKRGTEKKGILNSFIDMIAAVFAPTLGVLAGSGLIKGVLALCTSL
                     NLLTTESGTYIILNAAADAFFYFLPIFLAYTAAKKFNTDRFIAMVIAAALVYPTIVSA
                     YSDSITLRFLGMPVILARYTSTVIPAILAVWVLSYIEPKIRKSLHESIRNLLTPFICI
                     IVMVPITLLVVGPIADYASQLIAAGYLAVYNFSPVLSGAVIGGFWQVLVIFGLHWGLV
                     PVMTNNLSFYGRDTLGPACMTAVAAQAGAVLGVFLKTKNKKVKSLSLSAFITALFGIT
                     EPAVYGVTLKYKRPFYIACICGAIFGGVAGAAGAGALAVATRSILSFPIYIGEGFVWL
                     VASYFLAMISSCMLTFLFGYKDEIEEESSKDIVLSTPAAGEIIDLSEVNDPTFASGSL
                     GEGFAIIPTDGKIYSPVNGEVSTVFPTKHAIGVVSEEGAEILIHIGIDTVNLNGKYFQ
                     SAVSDGKKVRKGDLLMEVDLQELIKEGYDPTTMVIVTNSSRFKNIITSHKKNNQTRKL
                     ASLGGSI"
     gene            complement(16728..18503)
                     /locus_tag="IWO89_000013"
     CDS             complement(16728..18503)
                     /locus_tag="IWO89_000013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
                     /protein_id="HAQ0454807.1"
                     /translation="MFITKRRCKQMDLNKRQLQILVVLRNKNHWITSEELAEALGTNK
                     KTIQTEIKNMLAIYEKKIIIQSNKRSGYFLESIESETKQQIIQEVTKHKIYSSMDFRA
                     STIVTYLLFQKGYVSMQKIADIFFLSKTAVSLQIKTILRWIERNPKLELEVSSTKGLK
                     IIAEENSKRIFLSLVGTETVIQHARLPQQISTIFSNIMPTVQKTLKEVLISYNYIVSG
                     EDFIRFSRYLVLTILRREELLEKTNRYTLFIPMVETLTKKLSSELPYSFSNQEKQAIE
                     NRLLELNYLMINDEKNKEIEKNLRAFEQTIIRFLDLPVSLLFHETEFLLTHIKQMKTR
                     MDAGHNTMNHFVQKIISRYPLEIYLIRRFFPIHFQLRPNLAESAYFVLYLAEALNPFR
                     KQGEILIVSNQPISILNTLKKQIQQSMNMWIKECRIEPVYLFKNQSNKITEYDAILTT
                     EQELIFEWDDLIYLPSVLDEFESWEIYQILREKLTIVEEKKKTKLLDHYYKEENQIKI
                     HKKIDSIQQLISPSSDLVMLPLNAETLLCCLIKSQVETKITEYQLEIPLIHQQRKIKR
                     VFFVSFDYKRNDILVFFQAVSDLLG"
     gene            complement(18553..20049)
                     /locus_tag="IWO89_000014"
     CDS             complement(18553..20049)
                     /locus_tag="IWO89_000014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HAQ0454808.1"
                     /translation="MDQEIFPENFLWGGAVAANQCEGAWLEDGKLPNVTDTLIGIMNQ
                     HPSIQWNEEKKIWEIALDESLHYLSHEAIDFYHRFEEDIRLLKELGLKAFRTSISWAR
                     IFPRGDEQKPNEAGLVFYDRLINTLNRYDIEPVITLSHYETPLALVGEYGGWQNRKLI
                     DFFEFYAQTVFERYQGKVKYWMTFNEINNAFRMPYAAAGLVTFPARDKLEPIATLTKK
                     VIYQACHHMFLANARATQLLKKIDPNAKMGIMCSFSALATYAYDCDPENVFGSLQFKR
                     NSWFFSDVMCRGHYPAYIYRTWKEEDCAPVMLDGDEQCLQANTADYIAFSYYRSAVYK
                     KEAEMRVDTGGANGFDNPFLKEKSPEPWSWPIDPLGFRYVMNELTDRYELPLFIVENG
                     IGLDEAPDELNRIHDPKRCEYLKIHLQEIAEAIKDGCQIIGYLWWGPIDIVSAGTGEM
                     RKRYGFIYVDRQNDGSGSFRRLKKDSFEYYKQIIQSNGKNLEYRSFFK"
     gene            complement(20243..21865)
                     /locus_tag="IWO89_000015"
     CDS             complement(20243..21865)
                     /locus_tag="IWO89_000015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase"
                     /protein_id="HAQ0454809.1"
                     /translation="MEQKWWHHSVVYQIYPRSFQDSNQDGIGDLRGIISRLDYLAYLG
                     IDAIWLSPVYESPNDDNGYDISNYQAIMAEFGTMEEMEELIEEAKKRNIRIIMDLVVN
                     HTSDEHPWFIEARKNKENPYRDYYIWRDPVNGKEPNRLRSIFSGSAWQLDEKTGQYFL
                     HLYSKRQPDLNWKNKQVRQEIYQMMNFWIDKGIGGFRMDVIDMIGKEPDKEITSNGPK
                     LHEYLQEMNQATFGDKNLMTVGETWGATTEIAKLYSNPKRNELSMVFQFEHIGLDQQE
                     GKDKWDLKPLSISELKKVLSKWQTSLGDEGWNSLFWNNHDLPRIVSRWGNDDVYRKES
                     AKMFAILLHMMKGTPYIYQGEELGMTNCPIDDIAEAKDIETINMYNERISSGFTKEEI
                     LESINAKGRDNARTPMQWNAREHAGFTTGIPWLRVNPNYKEINAEEALADQDSVFYMY
                     KKLIDLRRKHDIIVWGDYELVEKTPEHVFAYSRIYEDQQWLVICNMSSEKQTFTVPRE
                     ALRVIVSNYPLKQLPDGKVDLRPYEAFVLEVK"
     gene            complement(22314..23627)
                     /locus_tag="IWO89_000016"
     CDS             complement(22314..23627)
                     /locus_tag="IWO89_000016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NCS2 family permease"
                     /protein_id="HAQ0454810.1"
                     /translation="MKEKILSYFEIEKLNTTVKREVLAGFTTFISMAYILFVNPTVLG
                     ASGMDEGAVFTATALASAIGCVLMGVLARYPIGTAPALGINAFFAYSVCLGMGIPWET
                     ALAGVFVASLIFILITIFKLREMIIDAIPADLKYAISGGIGLFIAFLGLSEGGIIVSN
                     ESTLVGLGSLNVGSTWLTIFGLVVTAILLVRRVPGGIFIGIAATTILGLVTGLIPMPS
                     EIVAAAPSLKPTFLVALKHVGDINSLQMWVVVLTFLLVTFFDTAGTLVGLANQAGFMK
                     DNKMPRVGKALASDSTAMLAGSLFGTSPVGAFVESSAGIAVGGRSGITAITTGIFFLL
                     GLFFSPLLSVVTSQVTAPALIMVGVLMAQSLRQIEWGNLEIAIPSFLILIGMPLTYSI
                     SDGIALGFIFYPITMLAAKRGKEVSPIMYGLFFVFVGFMWILNVK"
     gene            complement(23872..24096)
                     /locus_tag="IWO89_000017"
     CDS             complement(23872..24096)
                     /locus_tag="IWO89_000017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0454811.1"
                     /translation="MDLMGRLEGYLKYRRAKHSEKLRAKNLKSGKKKTKGWGKMVDTG
                     FGGLNNLNPVDTKEFIKEEQKRNGKIKSHY"
     gene            complement(24116..24484)
                     /locus_tag="IWO89_000018"
     CDS             complement(24116..24484)
                     /locus_tag="IWO89_000018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxalase"
                     /protein_id="HAQ0454812.1"
                     /translation="MIQKTRVMLYVNDVEMICRFFVEKIGAEISETIELPEEFKSIVL
                     SISKELELGVFPKVFVQKFSPEVLGPPPSLVFFTDEFETIYENMEEPGEITDNNGILT
                     FNFSDPEGNYFVIGKAESSD"
     gene            complement(24682..25392)
                     /locus_tag="IWO89_000019"
     CDS             complement(24682..25392)
                     /locus_tag="IWO89_000019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0454813.1"
                     /translation="MKKVIVDTNFWEVFPEATIEILSVNGIDNHVTKESEETYHQLLN
                     EAAKEARNYLTEETFSQNEVIAQWRQAFTTFKTKKGARSSIEALLKRANQEREFFPIN
                     PLVDIYNSISLSFGVPCGGEDIEKIAGNLHLGKAQGGESFLPLGADSDAPALPEEIIY
                     YDEQGAICRCLNWREAQRTMLTEETQAAVLVIESINAEQAARGAQAIEALKEKIDQTF
                     GVTSVRHTLTKDESEAEL"
     gene            complement(25410..25907)
                     /locus_tag="IWO89_000020"
     CDS             complement(25410..25907)
                     /locus_tag="IWO89_000020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAQ0454814.1"
                     /translation="MTPSDVDLLQQISIETFTDTFGKDNSPEHLADYLTQAYALEKLT
                     EELKDKETDFFLVSIDQEPAGYLKLNTGTSQSEEMADHFLEIERIYVRKKFKHQGIGT
                     FLLDTAIEKAKEKQKTAIWLGVWEHNDVALAFYRKKGFIKYSEHVFYMGDDPQTDYLF
                     IKQLF"
     gene            complement(25944..26396)
                     /locus_tag="IWO89_000021"
     CDS             complement(25944..26396)
                     /locus_tag="IWO89_000021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003331879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HAQ0454815.1"
                     /translation="MAEILRDIGMIARALDSISNIEFKEVDLTRGQYLYLVRICENPG
                     IIQEKLAEMIKVDRTTTARAIKKLESNGMIERLEDKENKKIKKLYPTKKGAEIYPFII
                     RENNYSNAVALNGLSDEEAKQLEYLLNKVCKNVSEDWNFVKKGNKRIY"
     gene            complement(26531..27136)
                     /locus_tag="IWO89_000022"
     CDS             complement(26531..27136)
                     /locus_tag="IWO89_000022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HAQ0454816.1"
                     /translation="MNKKLAQYKRLLALSQAGLYYGKDVFDKERFEEIRTISLELISE
                     IGKETFEEIKALTTIGEGHPTPKVDVRAYIKKDGKVLLIEDKRTKEWSLPGGFAEIGL
                     SPEENVRKEVYEETGLTVETTQLRAVFDTNKQKDIPQLFQYYKLVFACAIHGEEAKFI
                     ENNETSNMGFFSIEELPKLSEKRTTKKQLLILENKNQIYCD"
     gene            complement(27586..28206)
                     /locus_tag="IWO89_000023"
     CDS             complement(27586..28206)
                     /locus_tag="IWO89_000023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HAQ0454817.1"
                     /translation="MKKYFILLAGSPGTGKTYLMNKLRERFPDMYAITLDEIKEYYAE
                     SIGFNNLEERAEQERTKVYPFYYKALDLYMEAGKKVIVSEYPFSDKQKARLQELADRY
                     GYQVITIRLTADFEVLCQRRYKRDRESDRHLSYIMDHYHYGDVLEDRTKGTNHITKEA
                     FKKIIADRKYDKFQLGTLYEFDVTDYTQVDYTPLLDKLEKEINDNK"
     gene            complement(28208..29077)
                     /locus_tag="IWO89_000024"
     CDS             complement(28208..29077)
                     /locus_tag="IWO89_000024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphate aldolase family
                     protein"
                     /protein_id="HAQ0454818.1"
                     /translation="MYTTLKEVTKTAEELNFTVGAFNTHNLEMLPAMLGAAKEMGSPI
                     IIQTSVDTAKYIGMHVLVNVMTAIAEEEMVDAVLHLDHARDFDDIKEAIDSGYTSVMY
                     DGSHLPFKENILKTKAVVEYAHAHGVSVEGELGTIGGTEEGIHVAENDKVYTRPEDAV
                     KFVEATGVDALAIAIGTNHGQFKSKTEVNIPLLKEIDAVVDVPLVIHGGTGVKEEDYP
                     ELINNGIRKFNVGTELLVNWTKVAKETFSETEINKSLRHNVIPANEAVKEIVKHKIGL
                     FMNLESPINLGNK"
     gene            complement(29100..29507)
                     /locus_tag="IWO89_000025"
     CDS             complement(29100..29507)
                     /locus_tag="IWO89_000025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HAQ0454819.1"
                     /translation="MFGFFKKKEKEPKKVLAKEEKALSPEATEKIQSEITQLKQEIST
                     TQDKHKLAKLYEQVGLKFSELYVNDQAIQYLEKSLENKQTIGDGYKKLMSLYNQKRAD
                     AARAGDDQGIDYYMGKMDEMRQIAKQVTIKGNK"
     gene            complement(29526..30815)
                     /locus_tag="IWO89_000026"
     CDS             complement(29526..30815)
                     /locus_tag="IWO89_000026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018378547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAQ0454820.1"
                     /translation="MGVINFIIENILTQASITIALIAMLGLLLQKKSAGQVISGTLKT
                     LLGFQVLSAGSSIIVGSLTYFGKIFTEGFHMQGIIPSIESINGQAMNDLGLGRDIALT
                     FLAIFVFNIILARFTKWKYIFLTGQAILWMATMTTVFGYFAGLRGIVLILVGGFIGAC
                     FAIAMPAVAQPIIRKITGSNDIALGHFCTIGYLFEAGVAKLFGEKGENKKSIEDIKLP
                     THFEFLQDTYLSVMVVMVPLYIITVLFAGEPFASELSGDQNYIMFAFLQAIQFVVGVY
                     VLLAGVRLLLGEIVPAFRGIAMKLVPDAIPALDCPVFFPYSPNAVILGFITTTIGTII
                     AMFTLPMFGLAMILPGMLTNFFAGGTAGIFGNAVGGRRGAIIGGIAHGFFITLLPALL
                     VTIFNSMGFINATATDVDTVAAALLYAWILSPVLKAF"
     gene            complement(30842..31144)
                     /locus_tag="IWO89_000027"
     CDS             complement(30842..31144)
                     /locus_tag="IWO89_000027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015509266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAQ0454821.1"
                     /translation="MNKLNILFVCGAGLGSSFAAQMSAEDVLNKLGVEAKLDHCDISS
                     AVSMKPDVIITAENFRSQFEKFAVDPKTAMIYLKNIVSKNEIEEKITPVLQDKGVI"
     gene            complement(31169..33262)
                     /locus_tag="IWO89_000028"
     CDS             complement(31169..33262)
                     /locus_tag="IWO89_000028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator"
                     /protein_id="HAQ0454822.1"
                     /translation="MKTRTIEILHRIMTAQYPVKAKHLASEFNVSQRTIRQEVLEANI
                     WLSSKKLPEIRTIRNKVFLLKLEKEKQARLETEIKDVKDGFLNRRERIFDLVLAVAYS
                     RIPIHLNKKEEEFQISKSTLDEDMRRLRATLVSYGIEIVSYGKQGLVYKGSERSIRTM
                     IYDFINQNLGMTDFFMNQDAQLTPAQKIFLHYFPLVEIKKIQEIYSNKLLKQEDDIYK
                     KQILLFTLIWIQRIRKHEFISAINWKNTEIKDSSFSAFIDHMIEEFQLDNVPQVERNY
                     LRFTIETFNARDISNSIEWVQAQLLTIQLIQFVENETHVPFHLKEEALFENLYKHMAA
                     LIVRIKNHIQVMNPLKENIRTNYFPIYRAVTHFVPTIEEVIGGKILEDEIAFLVIHFS
                     TIASVIKRDLNYVYKSVVVCNHGMATGNLLAENLKEKFPQIEITAVLSSKEIELVDKL
                     DVNLVFSTFQLNYRNKPLLVVDPILTETNILVIEDFLKRNEELQRLEPNSHESTELFT
                     QIIDVMKESGGRVDRIIYGKLEKLFETNNLEINKREIQPMLKDILTDSNIMIKEKATT
                     WEESIELAARPLVKENIVEERYVEAMIRAVNDYGPYIVIGKHMALAHARPEDGVNKLG
                     VSVATIEQPIDFGNPEMDPVKIIFCLAAVDSYSHLNIMKELIELINNEEKLDRLIECT
                     NIEDFKQLLFSVVEK"
     gene            complement(33751..34515)
                     /locus_tag="IWO89_000029"
     CDS             complement(33751..34515)
                     /locus_tag="IWO89_000029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIM44-like domain-containing protein"
                     /protein_id="HAQ0454823.1"
                     /translation="MKKINYLAIILLVGLCLFALPTQGFAVAGGHGGGTSSGRSSGRG
                     SFGGRSYGGSYFGSRSYGRDSYGYYGPGTGPSSYEQTGFLALAVLSVGGVSAFSIVKN
                     KRQKKRLKAEILSRMSGTAKEKKAQFLEIQKIFMKIQSAWEEETPEKARSCYTDKLFA
                     EHQKVLQKNKADGVRNHTKKITIQEVGNYRQIHENSFAIRIDFRCCDYSVDRKDGRLI
                     SGSKTQRQYFSQIWYFDFSEKEKRWQADFIQPIMLD"
     gene            complement(34626..35495)
                     /gene="mdcB"
                     /locus_tag="IWO89_000030"
     CDS             complement(34626..35495)
                     /gene="mdcB"
                     /locus_tag="IWO89_000030"
                     /EC_number="2.4.2.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="triphosphoribosyl-dephospho-CoA synthase MdcB"
                     /protein_id="HAQ0454824.1"
                     /translation="MINDERKKAVTQQLAALVEEALIAEVTLSPKPGLVDALDAGAHS
                     DMDYALFLKSAKTLTPFFEEMAQIAWHHAIDQELRERIAEIGRKAEKAMLIATNGINT
                     HKGAIWAMGLLISVIAQKISMEQPFSLSKILDDVSKLAAFPDTKYRHKKTTHGHRVKQ
                     KYGVNGAYEEAILGYPHIRLAIQAAKELDNDSYVQKQMHMLLVLMSSLDDTCVLHRSD
                     QKTLVEMQKLAHAASQSALPNRPFTALLHFCQENQISPGGSADLLSASLFMLKIEELW
                     TADTYICPMLLND"
     gene            complement(35520..36596)
                     /locus_tag="IWO89_000031"
     CDS             complement(35520..36596)
                     /locus_tag="IWO89_000031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HAQ0454825.1"
                     /translation="MFENKKIKINVSDFQKVSAVTAVMMQTILSFALANSHSATNTAF
                     LGTFYVIVKYTAPMFIFAIVYNMVKTSQHLSYWEFLKEKFFELVVPYLLWTTAYLWLF
                     PAVQQQTPYTDTASFLLKYVTGDGAPHLWYTVMMLQIQLFMPFFVWLGYKVFANKKYV
                     WPVLIGSTILYVAWYLFYQTQVLTGPYHDSWYLLDRFVASFMIYGVAAFVYHEKVYQY
                     LDRVRYLFLPVALVIAFFSVRSLLAHPGDLSFANAPYLNTIQSLYSLVIIFAVFIGAS
                     KMIVNDSPKLPLFKWLSVYAYRTYLANVFVFQVLLLLFKDSWLQLPSGVMILVAYLMT
                     ASCAFALSWLLHIIWVAIKKGFSK"
     gene            complement(36620..37213)
                     /locus_tag="IWO89_000032"
     CDS             complement(36620..37213)
                     /locus_tag="IWO89_000032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malonate decarboxylase holo-ACP synthase"
                     /protein_id="HAQ0454826.1"
                     /translation="MIQAHDIVLFDKHEIEGFTLPEWVVLTEKMFGTVRRASTDHAEI
                     LPIGLRGPARNQRWSTEISKHAVKQVIHPWDIIEQESYRQEKIMDYPVYQQFHAARNI
                     LSGCKWGIGGSLGFELSTGIPAVKETSDFDLLLYANSPIELPIQVIQSHPAFFEQFDT
                     QVVTRQGGFSLKEYLREPEKKILLKTVEGPKLTKELW"
     gene            complement(37217..38893)
                     /gene="mdcD"
                     /locus_tag="IWO89_000033"
     CDS             complement(37217..38893)
                     /gene="mdcD"
                     /locus_tag="IWO89_000033"
                     /EC_number="4.1.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin-independent malonate decarboxylase
                     subunit beta"
                     /protein_id="HAQ0454827.1"
                     /translation="MKNSFVELNARERVNALLDNGRELIDPFDQIIAPSLPAQGIVPE
                     SDDGIVVSRGTINGKQAVVIAMEGKFQGGGIGEVSGAKIVAALEHVLEENKKGNEIFP
                     VIVLDTGGVRLQEANYGLLSISEIGNVIIALKQYVPVIGLIPGRVGSFGGMSITSALM
                     SYLIATKKARVGLNGPEVIEQEAGVREFDSSDKDLIWNTLGSRQRVESKIIDELVTDS
                     VEAIKAAVIAAMDERKDTHRSESSAFYLSLLDSLDLSKPMAIDDYNEAIANHQAKSID
                     LPEVAEGNEPAAASVGYDWFAALTGMKNPTSSISTVYYGKSSKFIEEAIVTSIVPDPK
                     NPMYRVRNGEVGLVEGFRMGQILDHVYEEDKDKKVKRPIIVVIDVPSQAYGYNEELIG
                     IHVALANSAAAYAKLRQAGHPVIGLIAGNAISGAFLAHGLQSSRLIALNNKEITVQAM
                     SKESAARVTKRSVAEIEAAAEKVPAIAYDINNYTKLGAIYHFLEDITDQAASEQNVSK
                     VIEAINEAIQDVRNTNDTMLTNRYTNETAKTFGRVETNKVRAKMDEEWDA"
     gene            complement(38883..39182)
                     /locus_tag="IWO89_000034"
     CDS             complement(38883..39182)
                     /locus_tag="IWO89_000034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malonate decarboxylase subunit delta"
                     /protein_id="HAQ0454828.1"
                     /translation="MEQLAYKYPTKQPINKKIHVGVVGSGDLEILMFPTEKTESTVKI
                     CTGSDGFGEVWNNVLTRFFDRYPISADIVINDFGATPGVVYLRLTQAMEELSDEK"
     gene            complement(39284..40930)
                     /gene="mdcA"
                     /locus_tag="IWO89_000035"
     CDS             complement(39284..40930)
                     /gene="mdcA"
                     /locus_tag="IWO89_000035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010748322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malonate decarboxylase subunit alpha"
                     /protein_id="HAQ0454829.1"
                     /translation="MEKPNRNWASKREKKAQRLEKISNLLNGKYVETEKIVEVMEKLI
                     APGDKVVLEGDNQKQASFLSESLEKVDAEKVNDLHLIMSSISRPEHLNIFEKGIASKI
                     DFSYAGAQSLRVAQMIEDGTMKLGDIHTYLELYGRLFIDLIPNVVLVAADKADKDGNL
                     YTGFNTEETPTIIEAAAFKDGIVIVQVNEVVDSLPRVDIPGDWVDVVVKADKPYQLEA
                     LFTRDPQNITELQILMAMMAIKGIYAEHGVQSLNHGIGFNTAAIELLLPTYGESLGLK
                     GKIAKNWVLNPHPTMIPAIESGWVESIHSFGGEVGMEKYIAARPDVFFVGKDGTMRSN
                     RTLSQAAGQYAIDMFIGSTLQLDYEGNSSTVTKGRLSGFGGAPNMGHNPGGRRHSTPA
                     WQSLRDNDDPLGKGQKLVVQMVETYGSNKKPVFVEELDALAVQKQAGLANAPVMIYSE
                     DTTHIVTEEGIAYLYKAKTKEERQAAIAAIGGVTPLGMTSTKESLDSLRAAGIVKLPE
                     DLGIKRSEAKRSLLAAQTIDDLIEWSDGLYEPPMKFRTWS"
     gene            complement(40937..41848)
                     /locus_tag="IWO89_000036"
     CDS             complement(40937..41848)
                     /locus_tag="IWO89_000036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HAQ0454830.1"
                     /translation="MSTGFLFPGQGSQKLGMLADIPEMYMERLENVTGYRLVDKEENY
                     QDTVFIQLALLTKAVFYLDEMEKNGIQPDIAAGHSIGAFCAAVACRSLRFENAAELVY
                     HRAVLMKEAYPSGYAMGVVVGVTRSEAEEIVAKTFDPDHPVYLSNENCPMQHTLSGSI
                     EGLSVTLAEAKRHQAQTAKLLKVPVPSHCLLMNDTVKKFKPYMQSVHLSDARCLYIKN
                     TDGRATQDAEEIRKDLLENLAFPVQWNQMMDLAKELGMDLTIEFPPGNTLTKLIHTKF
                     GVDKGIRTINLDQHGIDDATFLYQKWR"
     gene            41968..42825
                     /locus_tag="IWO89_000037"
     CDS             41968..42825
                     /locus_tag="IWO89_000037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HAQ0454831.1"
                     /translation="MKLLHLQYFKKVFETKNVTQAAKELFISQPALSRAIKHLENELG
                     VPLFYHSGRNIETTVYAEEFYPYAVKTLDTLEEGIKLLSSVNEKIVTSVILYLEVASV
                     SIPNLVRIFMEKHPDIQLTIMQHHLPDDITDPVLYITSEMKPGMTNIPIMKEPVYVAI
                     PKKHPLAKKEHLQLEDIQRTPILMLSKSNAFRHTLDAALEAKSIELTISSTTDDPATL
                     RSIVKQGLAVSFFPKVSWSYDKNDPFVLREIKDLPLTRTIYLSSSFTEDHPLIKMIAK
                     TMREFYLGK"
     gene            complement(43012..43374)
                     /locus_tag="IWO89_000038"
     CDS             complement(43012..43374)
                     /locus_tag="IWO89_000038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0454832.1"
                     /translation="MLTKKELIDHINETYNLSPEYIFKKFPDYCVFRHQKSKKWFGVL
                     MSVPEEKIYDDASDHRIINILDLKVDPELGGILRGKEGYFPAYHMNKEHWISIDLRKI
                     EKLDELADLIDGSFKSTQ"
     gene            43720..45159
                     /locus_tag="IWO89_000039"
     CDS             43720..45159
                     /locus_tag="IWO89_000039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
                     /protein_id="HAQ0454833.1"
                     /translation="MYSLMKKIITEKDIRRHVSLVEQLLNHTKITVNELAEIIGTTER
                     TIFSDLQSIRSQLPEGWDIISDQAGISLQNQQNLLTNDLWEIFFKQSVSVELLKNLLF
                     TKKVAVPDFLADHGLSYGTLKRHVTKINQRLASYDLQIDLTKYTACILGKERAIRTFY
                     HRLLIPFTHNNYFFEDYSIHESHYFQFLRNLSQTELAVETEEIFGTCWFFINTIRIKA
                     NCRLDSSIHTNSTLSSLYDSALKKLYLTEGIYLKDTELSFASFCFLESWNYNNNYGQE
                     TARCLHHSPFLEVLETFVEELASELSLDQLKKTSLTDNLALLILKYHESPILIEALDR
                     QYHRFLETYDQQYAHLYQQKENLLNDLKKEIEINEPNYFLQLLSLLIQQTIFSIKPNL
                     FNVYFIFQGEPSWKAFLQQELKDYLGKRVCFLPIELTDLSTISFEKTDILISNFPLDS
                     LDIPIVYISSIPTKNELNRLTELTFKTFL"
     gene            complement(45166..47718)
                     /locus_tag="IWO89_000040"
     CDS             complement(45166..47718)
                     /locus_tag="IWO89_000040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IC family P-type ATPase"
                     /protein_id="HAQ0454834.1"
                     /translation="MNWYRLTVEDVLKNYQTSTEGLSQEERQKRLTDQGYNEIKARQQ
                     TKRWKKIAKHFTDLLMIVLIIASFLKFASSEYIEGSIILFVVIVNGLVSYWQERKAEE
                     SLNGLKQLMGQEAIVLSNGMQEKIPAKELVKGDVIVLRAGDVVPADIRLIEAHDLLVE
                     ESILTGESEASEKSHAVLTEEESIGDQKNMAFSGTLVQSGSAVGVVVETGNTTEIGKI
                     NQALQSVEQQTTPLIKKINRLNKQIFQGILCLSLFLVIFTTFRYGMDWHILLSATIAL
                     VVSMVPEGLPAVLTMILSVGVHEMAKEKAIIKGLPSVETLGSMTVICSDKTGTLTKNE
                     MTVVDVAAKEEACVLSIMKNCQELKTKEEQKTENLSGNPTEVALIRHTETANLPLKKI
                     EAKIPFSSEYKYMATMHAEEKGAVIYVKGAPEVLFAKSTLSSAEQEAWSQTAAEFARK
                     GQRVLGFAYKKVDSKQELTHETLTQLTFAGIAGLIDPPKESAVKAVKECQQAGISVKM
                     ITGDHKDTAKAIAEQIGLKHTAKVLEGIDLDLMSDEELIQQVPIVDVFARTTPEHKLR
                     IVKALQKNGEIVGMTGDGVNDAPALKRSDVGIAMGIKGSEVSKQAADMVLGDDNFHTI
                     AKAVKEGRRILDNLQKTINFFLPTALAQGLILIWALMLNRPLPLSPVQILWVNMVATI
                     TLSYALGFESPDPEIMKRPPRDPKQGILSGYHLFRIIYVSLLIMVPAYLLAMQFEGQS
                     LQQTILLQNIVLAQAVYMINCRELSKPSLNQGLLQNKALFLSLGILALLQGMVIFLPV
                     GQQLIGTVSLTMTQQLLIGANVILLFLVVEIEKGIMNKLFRKKEKTAREYSR"
     gene            complement(47832..49163)
                     /locus_tag="IWO89_000041"
     CDS             complement(47832..49163)
                     /locus_tag="IWO89_000041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAQ0454835.1"
                     /translation="MEKPAMKRTLTNRNIQLIALGGAIGTGLFLGSAKAIASAGPAVI
                     LVYLICGLAIYGLMRAMGDLFLANSTFNSISDFVDYYLGKKWAFFVNWTYWLCWLGAG
                     LAELTAIGLYIHFWFVHVPALISGGSALMILMAVNLVAVKWFGELESFFSIIKILGIF
                     LFILLGIGLSVSDFHTQSIGIKENFGQIDELLPFGFAGMVVSFPMVLFSFAGIEMIGL
                     TVGETSDPQKNLKKAINCLPWRILFFYIGTILSILFVVPWHCLDLKESPLVTMLQVIH
                     CQGGAAIVNLMILVASLSSANSAVYSTSRILYQTAKEKSLPHWFCALSKKGVPLHGIL
                     ITGMLFFAGLCLHFFVADSRLFFQLLAGMTTSCFLFVWSSILCAHVQFIKEKKRNTTQ
                     RYKDLALLVFFTSIAFSLLFESTTRFIPFLLVIWFVFLNFVYKRISNRNIS"
     gene            complement(49129..50607)
                     /locus_tag="IWO89_000042"
     CDS             complement(49129..50607)
                     /locus_tag="IWO89_000042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAQ0454836.1"
                     /translation="MKDTGSSKQITTMTFIGMTCALVASVRNIPDVAATSWTMIFYML
                     VAVLLYAFPISLISGEFAGMFPQKGGPELWVSNALGKKWGFVVSWLLWVQMFPGMVMV
                     ASALAPLFGNIIDNVPLGLNSKFTLVVILVVYWIITLLNLKFDMAKIGGKVGVWLGLY
                     VPLTMMLLLGFAAWIKVGIVPTGTLGSFSWDKLIPDTTTASSFVYFAPIMFIFTGIEM
                     SSVYITRLENPVKTYIRGVFAALIFIFFVNILNALVVANVVPKGQMELNNIAQSISIY
                     CQILGLPHLIVNLFSLLVFIGVAVQLSAWASGPAKTVTESARKGAYPPKFNFWKTNQF
                     DVSKSVILTQSVIISIFALFYLLIPGVNQAFLMLVNSTTVIYCIVYVIMGIAVLRLRY
                     THAKLNRPFRIGKKEKKSNLGVWTVVIVLFAAIGFSVGLTMKAGTWINLIAVTAISVI
                     LFVVPLCIEKIKKPSWEQEVAMLLEKEKGELDGKTGNETDSN"
     gene            complement(50912..52747)
                     /gene="tdc"
                     /locus_tag="IWO89_000043"
     CDS             complement(50912..52747)
                     /gene="tdc"
                     /locus_tag="IWO89_000043"
                     /EC_number="4.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine decarboxylase"
                     /protein_id="HAQ0454837.1"
                     /translation="MKDMDIKAVFIGDKAENGPVYKMLLNKMVDEHLGWRENYIPSDM
                     PAISEGDKLTPDYLATRDHMIEVLDEVSQRLRAGSIPWHSAGRYWGQMNAETLMPALL
                     AYNYAMLWNPNNVALESSMATSQMEAEVGQDFASLFNMTDGWGHIAADGSIANLEGLW
                     YARCIKSIPLAVKEVLPEKVKKMSEWELLNLSVEEILEMTESFTDEEMDEVKAASSRS
                     GKNIQRLGKWLVPQTKHYSWMKALDICGVGLDQMVAIPVQEDYRMDINALEKTIRELA
                     GQKIPILGVVAVVGTTEEGQVDSVDKIVQLRERLKDEGIYFYLHVDAAYGGYARSLFL
                     NEAGEFVPYASLAEFFEEHHVFHHCVTIDKEVYEGFRAISEADSVTIDPHKMGYVPYA
                     AGGIVIKHKNMRNIISYFAPYVFEKSVKAPDMLGAYILEGSKAGATAAAVWTAHRVLP
                     LNVTGYGQLIGASIEAAQRFREFLDHLTFTVKGKTIEVYPLNHPDFNMVNWVFKEQGC
                     TDLNAINELNEKMFDRSSYMDGDVYGERFITSHTTFTQEDYGDSPIRFVERMGLTKEE
                     WKKEQKITLLRAAIMTPYLNDDRIFNFYTKKIAKAMEKKLNEIIQ"
     gene            53217..54644
                     /locus_tag="IWO89_000044"
     CDS             53217..54644
                     /locus_tag="IWO89_000044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HAQ0454838.1"
                     /translation="MIEEYIEKNILRQLFLCGQFYVNKEVNLEKLSNLLHVCKTTLLN
                     DINNIKKEFEEQIAYTHREKDRYTLYFSEHIPRCKIMQQLSQNSLFLKTCLLYLEEDE
                     PDYLQLTECEFISVSKAYSLKKQVLAYFNDCGIEIDRYSPRFTEMERRLLLLNVSYRL
                     GGFNSWELPESFFERADRFIESVTENSGRFYDKENKEILSIGFAISFLRQQVCAVTID
                     SKFIEEIKKRPIYNYVESAWENTDFQTYYKKEEFAFILTLFNLCNYGFHSYQLIAEDF
                     QQLHQVFIDNTPEIKELVATFESHFNQELFGNQPFERALIHLMRSAWDNYQLFMPEKF
                     YLLNEEQTNLYKEVQTIFSSWSSQLPYDLRLNPNCMRAFVIELSGILRLTKEHLTIYI
                     VTNSDVHYLIYREALEAVTTFDFQVAPTIYSSISDIKKYAQQSSNRVLCERTLYTPDA
                     VQYENIIPISINTIDRAIISAVQNK"
     gene            complement(54736..55101)
                     /locus_tag="IWO89_000045"
     CDS             complement(54736..55101)
                     /locus_tag="IWO89_000045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1622 domain-containing protein"
                     /protein_id="HAQ0454839.1"
                     /translation="MTTELMQFLLPYFEKLVLGLDIFAICLLVWGVCLSIKDFLKYLF
                     VHTKVQSDRVSENNAIKKMLGGYILLSLEILVSAGIIESIIKPTLQDIFQLAALVVIR
                     TIISYFLNKEIGPLDVQNK"
     gene            complement(55098..55463)
                     /locus_tag="IWO89_000046"
     CDS             complement(55098..55463)
                     /locus_tag="IWO89_000046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1622 domain-containing protein"
                     /protein_id="HAQ0454840.1"
                     /translation="MFRSNLIEFLEWIIAILNICSIGILIVGIVLVLFKLFDWSHVKK
                     NYAERNTRNADTRKLLASYILLSLEVLVVADIIESVIKPTWTDILKLALIIIIRTVIS
                     YFLNQEIEDKEKQRRGEVG"
     gene            complement(55655..55903)
                     /locus_tag="IWO89_000047"
     CDS             complement(55655..55903)
                     /locus_tag="IWO89_000047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0454841.1"
                     /translation="MKNASLFSLSISKDSSFLLECQMIGREEKGNFDKKEEVSDFPVN
                     KNRVGKKKRFEEVYGSIFRNLGTKKAPRQCFFEKLMTD"
     gene            55902..56030
                     /locus_tag="IWO89_000048"
     CDS             55902..56030
                     /locus_tag="IWO89_000048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methanol dehydrogenase"
                     /protein_id="HAQ0454842.1"
                     /translation="MSKKLIKVGIGLGLLALGAAYLGKKTGLFEDDSHLYDEFESI"
     gene            56087..57781
                     /locus_tag="IWO89_000049"
     CDS             56087..57781
                     /locus_tag="IWO89_000049"
                     /EC_number="4.2.1.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oleate hydratase"
                     /protein_id="HAQ0454843.1"
                     /translation="MRKSKAIMIGAGIANMAAAVYLIQEGKWKGDQITFYSLDDHGSN
                     DGAPTDTVVDEYWNKNHPLENQKGYIARGGRMLNYRTYVDLMDLLSRIPSVTEPGMTA
                     EEDTRDFDAKHRTFDKARLMEGGKGIIDGGKLGFNNKDRILLTKLIAMPDSEEEKLDN
                     ITIEEYFKDDPHFFETNFWFMWETTFAFRTRSSAQELRRYMHQMIYEFTQIEHLVGVN
                     RTRYNQYESIMLPLIEYLKEQGCKIILNRRVTDWEFKETPMQDEITVTGLKITNTLTQ
                     EEEYVVVDEDTAVIFTNGSITDSATLGDYETPAVENMDYGAASSLWKKASERFYNLGN
                     PDKFFADRDASEWVSFTLTTKNHLLLNEITRITTQEPGNALNSFISTTPITPLGQKDV
                     NMSIVVHHQPHFTSQKPNETVIWGYFLYPRRRGEFVDKQYIKMNGKEMLEELIGQLSK
                     VDPGPVNIREKETEIFDSVINNIPVYMPYASALFNNRAKSDRPKVIPKHSTNLAFTGE
                     FVEQPYQMIFTEQSAVRSGEIAAFHFAGIPMSRLVKTPRYDKDIPTLARAAKKMFE"
     gene            57881..58054
                     /locus_tag="IWO89_000050"
     CDS             57881..58054
                     /locus_tag="IWO89_000050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0454844.1"
                     /translation="MQAFEHRLPRGYEKAALHQVIRAAKLKQLLMFLTWLDLLPQMQA
                     FEHRLPRGYEKGV"
     gene            58432..59565
                     /locus_tag="IWO89_000051"
     CDS             58432..59565
                     /locus_tag="IWO89_000051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HAQ0454845.1"
                     /translation="MKAVTWQGKQKMEIRNVDDPKILEPTDAIIRITATAICGSDLHL
                     YHHGEAVLEKGYVVGHEPMGIVEEVGSQVQTLKKGQRVVIPFNISCGHCHFCLNHMES
                     QCDESNPDQLYGGLFGFGKLNGNHWGGQAEYLRVPYADSTSFVVPDNDLPDEKVLFLS
                     DILPTAYWSVENAGVKKGDTVVILGSGPVGLFAQKFAVMAGAERVIAVDPVQHRLDKA
                     ANYNGVETYFLEDTAQAGTELYELAKGGADVIIDCVGMDGLEPVKEKAKNLVSLQSGT
                     ISPLQMASQAVKKFGTVQVTGVYMTPASSYPLQEFFMRDIAVKHGQAPIIHLMPKIYA
                     MIAEGLFDPTQIITHSLPLSEAAQAYEIFDKKEDKNIKVVLKP"
     gene            59596..59916
                     /locus_tag="IWO89_000052"
     CDS             59596..59916
                     /locus_tag="IWO89_000052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0454846.1"
                     /translation="MPKIKRNTHEIEIGKEEDIEIRGRRFRFYFQNRYTLISLAVDLL
                     TGIFYIFGSLASLTKIPDRYGTYFYLAGAIFLTIRPILRIVRNIFIYDEKKAMKRTSK
                     KKEA"
     gene            complement(59945..60409)
                     /locus_tag="IWO89_000053"
     CDS             complement(59945..60409)
                     /locus_tag="IWO89_000053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAQ0454847.1"
                     /translation="MVTYNWIKLREHPELVKQSANWFSSKWGVPAVVYEESIRTGIAN
                     KESIPQWYVVLDEKETIVGGAGVIENDFHNRPDLTPNLCALFVEPEKRKQGIAKRILA
                     EIRKEMHQLGYEKLYLITDHTTFYERYEWTFLTMVKEESGTTARLYQSDTIG"
     gene            complement(60541..61794)
                     /locus_tag="IWO89_000054"
     CDS             complement(60541..61794)
                     /locus_tag="IWO89_000054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAQ0454848.1"
                     /translation="MDKFVAFMEKHFIPVASKIGAQRHLVAIRDSFMVSMPLMILGAL
                     AVMINNLPIPGFQELMNSIFGGESWKGFGAAAWNGTFAILSVLIAFLLAYHLANGYRK
                     DGVSAGVISLGSFFALGGALGMSSNGLFIAIIVGIISTEIFVRLSGNEKLIIKMPDGV
                     PPAVAKAFASLLPAMITISAFALVAAIFAAFGVDDIVGSFYTVVQEPFMGLANSYPSA
                     LLLAFITPFLWFFGLHGANMVDPLMQTINAPAIEANVNAIAAGHSAPFIVNKPFFDSF
                     VNLGGTGATLGLLLAIYLVGRKNKPYMVVTNLSIAPGVFNINEPTMFGLPIVLNPIMF
                     IPFILTPMVLVSVAYFATSTGLVPAATVMPPWVTPPIIGGVLATKSIAGGVLAAVNLV
                     LSILIYLPFVKVATIQERRKEALDA"
     gene            complement(61823..62140)
                     /locus_tag="IWO89_000055"
     CDS             complement(61823..62140)
                     /locus_tag="IWO89_000055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HAQ0454849.1"
                     /translation="MEDQKNLEAIMGLIMFGGNAKSDAMEAIAAAKKGDFTLADEKIK
                     DAEESLVQAHHSQTEMLTQEAQGNHVEVTLLTVHSQDHLMTSIAFTDLAKEIINLYRR
                     LNA"
     gene            complement(62166..62486)
                     /locus_tag="IWO89_000056"
     CDS             complement(62166..62486)
                     /locus_tag="IWO89_000056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAQ0454850.1"
                     /translation="MEKKTIMLVCSAGMSTSLLVTKMQKAAEAKGMEADIFAVSASDA
                     DNQLESKNVDVLLLGPQVRFMQNQFAEKLAPKGIPLDVINMADYGMMNGEKVLDQAEK
                     LINK"
     gene            complement(62950..64365)
                     /locus_tag="IWO89_000057"
     CDS             complement(62950..64365)
                     /locus_tag="IWO89_000057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HAQ0454851.1"
                     /translation="MKITIPKNFILGAASSAWQTEGWKGKKEGQDSFIDSWYKNEHFV
                     WHNGYGPAVATNFMEQYQQDVDLMKSIHLTHYRTSINWARFFTDYENCIVDEEYAQHI
                     SEMIDSLIEADVEPMICLEHYEVPAFLLEKYDGWSSRKVVDFYAKYAQIAFDRYGDRV
                     KNWFTFNEPIVPQTRCYLDAVRWPHEQNTKKWMMWNYHKVLATAKAVEIYRSGNYGGR
                     IGCILNPEMVYARSNSPEDQKAAYMYDLFYNRVFFDPMVKGYYTEEFLELCQKQSIYF
                     DPATEDLETIQHHTVDFLGINQYYPKRVRAPRYQWNEETPFHPERYYEVFDLPGKKMN
                     NSRGWEIYPKIMYDIAMYLKENYHNIPWLITENGMGREYEEQYMDEKGIVQDDYRIEF
                     IGQHLHYLIKATEEGANCEGYMLWAFTDCVSPMNAFKNRYGLVRIELNDERSRTLKKS
                     ASWYQTLIDKRELDLPDEIYK"
     gene            complement(64382..65698)
                     /locus_tag="IWO89_000058"
     CDS             complement(64382..65698)
                     /locus_tag="IWO89_000058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAQ0454852.1"
                     /translation="MRGSGMSFMDKLANVLGNVATRINNLRYIMVIKNAFAALIPVII
                     TGAFGTLFSAMVFDSENGLAQISFLRFLEELKPIASAISYVTLSFLTIYAVFLIGIEL
                     AKLNKVDGVFPGIIAVMSYLSVNPTVYELVNENQTVIVENVLAKQYTDTKGLFLGMLV
                     SILSIELYCWLRKQKKLQLRMPDTVPTNVSASFSALFPTILTVSGIAAAGFIIKELTG
                     MYLYDIIYNVVQRPLEGVVQGLPGILLLMFIAQVFWVIGIHGNQMVKPIREPLLLAAI
                     AVNTEAFEAGKEVPNIITMPFWDMYMSMGGSGVTIGLLIAVLLVGKREDMKQITKLSL
                     APGIFNINEPVIFGMPIMLNPILAIPFILTPLITGTIGYFATALGFAAKAVVMVPWPM
                     PPIVNAYLATAGNIGAVITQLVCIIVSILIYLPFVKVANRVENDGI"
     gene            complement(65848..66546)
                     /locus_tag="IWO89_000059"
     CDS             complement(65848..66546)
                     /locus_tag="IWO89_000059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FadR family transcriptional regulator"
                     /protein_id="HAQ0454853.1"
                     /translation="MEENKTLVEVTTDKILRYLKEMQLQVGDKLPNEYELSDYLEVGR
                     STVREAVRALASRNILEVRQGAGTFVSDKRGIANDPLGFSLIEDQDRMIRDLFELRYL
                     LEPKMAGLAAEYATKNQIVQMKKLTQKIETSFREGTQEHVQLDIDFHTMIAEASGNVA
                     LLHIIPIINESIALFNKNYDIQHLKIETIDIHTEIVQAIERRNALAAFDAMTVHMAIN
                     RRELQKIIGEKETN"
     gene            complement(66835..67824)
                     /locus_tag="IWO89_000060"
     CDS             complement(66835..67824)
                     /locus_tag="IWO89_000060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-glyceraldehyde 3-phosphate reductase"
                     /protein_id="HAQ0454854.1"
                     /translation="MYIADSARYEKMEYRKSGHSGLKLPILSLGLWQNFGDYDPIHNQ
                     REILRGAFDMGITHFDLANNYGGPAGAAEKNFGRIFREDFQAYRDELIISSKAGYHMW
                     EGPYGEWGSRKSIISSCDQSLQRMGLDYVDIFYHHRPDPDTPLEETAEALMQLVRQGK
                     ALYIGISNYNGEDTKKMTEILKRKEAPFIIHQMRYNMFSRALLEDDLSPVLEEEGLGA
                     ITFSPLAQGLLTNRYLHGIPEDSRAHRKEIPFLSEEQVGSTLEKIKALQTIAVSRGQS
                     LAQMALAWNLRQKSVTSVLVGASRLSQLQESVRMMDNLDFSPEEELRIDQILE"
     gene            complement(67904..68737)
                     /gene="msrA"
                     /locus_tag="IWO89_000061"
     CDS             complement(67904..68737)
                     /gene="msrA"
                     /locus_tag="IWO89_000061"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HAQ0454855.1"
                     /translation="MDEEQIFIQTLYNLILNPNTRDWERKVLIQTKNDIRENISVKEQ
                     LSKLEATLRPLAIRMNLTPDVMDFYLLITEGFDKEQKYDFSKHAMQDADYQERAVFAG
                     GCFWCMVEPFESKKGILSVLSGYTGGHVEKPNYDQVSGGYTGHVEAVEIIYDTREISY
                     SELLTIYWQITDPTDTFGQFQDRGKQYRPVIFYQVERQKELAEQSKQKLDSSGTFHQP
                     IVTKIEPAGTFWPAENYHQQFYKKQPKRYKKIQQARNQFLIYQRVKNKWQKNIRKNHF
                     D"
     gene            complement(68912..70546)
                     /locus_tag="IWO89_000062"
     CDS             complement(68912..70546)
                     /locus_tag="IWO89_000062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0454856.1"
                     /translation="MKINKKVVILSGLIIVSSIYSFIFFKNTQSIYTTGDLSFHLSRI
                     KGLSTIFTSPINYETFNYTGYGVNYFYPFLTFFPAVMLYWMTKNLIVSYIIYVWLLNL
                     CTTLVAYHYGERFLKQKKAAFLFSCLYIFSAYRTVDIYYRSAIAEAIAITLVIPVLFY
                     AYQIISGKEEKYPSVKLALSMSLLVYSHVLSTLMSTALIIIFIFIRLVSKGFKNADFI
                     AIFKKLFSAAGMTLVLTSAFWYPMFEQMLYQKINKPSVTNLYAHASNVFDSLTEAMNN
                     DLTTYSMGLVGLLSLCIPLILFKKLTNIEKKIYYGTCLTWLATTSLVPWYLLQNTPAK
                     LLQFPWRILSLQIIFSSLILTMIFFKNRRYNKTRELFYLFMTVLLLITLTVSSKKNFN
                     QKIISQPGHIVVNKETVEAYTTKGMDYFLYDYSPIASLENKEHIIKHEAKIGSQWVNL
                     PYKTTGSSIIYRVTNEKSQVITLPVYAYLGTNVEINHQPVKEQVNANGLVQISGVKGV
                     ADIKVSTSYTVTTIISYLVTLTAYLCLFFKIVFHKR"
     gene            complement(70533..72380)
                     /locus_tag="IWO89_000063"
     CDS             complement(70533..72380)
                     /locus_tag="IWO89_000063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002369935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0454857.1"
                     /translation="MFFFIFFSYLKVIKIDSFLVYHTSAMKNLTQNLLKKDNSSILKI
                     ELKKENNKSMKISKKAFLLVVLILLSTLYSVNFMRNAQEIYTTGDLSFHLSRIKGLSS
                     IFEGPINYTTFNNYGDGLNYFYPFLTIIPAVVFYGISNNLILSYVLYIWLLNICTILI
                     SFYYGEKFFKKIDAAFLFSCLYTFATYRTVDIYYRSAIAEAIALTIIPAVLYYAYGLI
                     FNREKKVIALSVSISLLVYTHVLTTLLCSALIACMIIVRLFLSKQIKNDIKFVVVNLV
                     KAVFLTCALTSYFWYPMLQQMNYQEIHSPFKTDLQLEALSISDSIIQALNNDITTYTM
                     GLLGLVALIFPLFILKEMEKKEKIIYVGVVSTWVCVTNLFPWFLFQKTPISLLQFPWR
                     ILGIQIFFSSMIITLVFMKKTMNKKYSWLIIGATVVFLLVTSIAAKENYAKVIQSKHG
                     HIVINEETLPRYTTSDMGGLYDYAPTEAIKERPHLEKHEVKVGESWEKGKYKAADNNI
                     TYTVASNKKQKVTVPVYAYLGTQVKVNGKVVNDSYKKHGLVNVDVNAGENKIEITTKY
                     TPTALMALWISILTYCLVVYSSFKRFAPNKLKDSRYVTKKEINKKNENK"
     gene            72367..74004
                     /locus_tag="IWO89_000064"
     CDS             72367..74004
                     /locus_tag="IWO89_000064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfhO family protein"
                     /protein_id="HAQ0454858.1"
                     /translation="MKKNKNQIIDIIFMLFLSFAYVIPLFMSKGIYHSVNQDTYFHLS
                     RIIGLDNVWSSPVNFNNFDHHGTMMNIFYPWLTLYPAFLFYKMMGNLVLSYNIYYFFI
                     TFLTMVVSYFSMKQIKNNRYISLLFSIIYTFSAYRAIDIFHRASLGEAVALTFLPLIL
                     MGCYEIYIRDYQKWYWLSIGMTLVVYTHLLSVAMVSVFIGGTLFLSFYFWDQKIARLL
                     SLLKATVLTFFLSAGFLIPFIQQSRAQKLKVPLGKELSGMAPSDMLTHILNNNYNNYT
                     IGLFLFLGLIGAFIFIKKLTADDLFIFFLGVFVLFCSTNLFPWQLFNHTPVKSLQFVW
                     RLNGFSSLFIAYTMSIVIYYIFSTKNNSWKIMVFTFLALILHLSGVSNSIHANKDSLT
                     LIAPEDAVAIAENYNHTDYANKESIYHPDMINNDQYLLGNQEINPTTHFMSNKLTIEL
                     DNSNNKNTVLTTPIYRYKGQVASINGKLVQTKLSKFGTTELTIPPGINKVVITYQYTK
                     LAIASRYLSIVTLILFLLYRFTFSKQKQPRREVQHSH"
     gene            complement(74149..74889)
                     /gene="truA"
                     /locus_tag="IWO89_000065"
     CDS             complement(74149..74889)
                     /gene="truA"
                     /locus_tag="IWO89_000065"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016175953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HAQ0454859.1"
                     /translation="MRNIKLTIEYDGKRYSGWQRLGDDDKTIQGKIEKVLHQMTNEEI
                     EIIGSGRTDAGTHARGQVANFKTNTEIGLAEMIDFMNRYLPRDIVIKRIEEMPERFHS
                     RYNAVGKKYSYYVWNNVIPSAFERNHSFYFPQELDMDKLNAACEKLVGTHDFIGFSAL
                     KKSKKSTVRTIDELSIERKGEMLHFTFVGDGFLYKMVRIIMGTLLEIGTGAMPIETID
                     EIFESKVRQHAGETVPAQGLFLDEVYYR"
     gene            complement(75080..75715)
                     /locus_tag="IWO89_000066"
     CDS             complement(75080..75715)
                     /locus_tag="IWO89_000066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF998 domain-containing protein"
                     /protein_id="HAQ0454860.1"
                     /translation="MDFLRKFGFFFLLLGVLSDFLTPYVLGLYYPELDQMKSVISVFG
                     EVSSPVRKAFLIWSVVSGIFFILSLPALYAAFVSTSRVLATLVTAAIAFYGIGDCIFT
                     GLFSINTHESSWNVSTWVHNIGSGLGYSGFLLFPVLLFLIYRKNGQMDVSHLYLGLSV
                     ISLLFAAIYGLARIPSINSWPLLNKIGFCQRISFFFNYVPIARLSILHLKQ"
     gene            complement(75862..77103)
                     /gene="sstT"
                     /locus_tag="IWO89_000067"
     CDS             complement(75862..77103)
                     /gene="sstT"
                     /locus_tag="IWO89_000067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine transporter SstT"
                     /protein_id="HAQ0454861.1"
                     /translation="MLEKFLDMSLVQRIVVGILVGILFGIFFPTWTFISILGTLFVSC
                     LKAIAPILVFFLTMASIAKHKIGNKTFVKPILILYLVGTLLSALVAVIVSSIFTVPIT
                     LQETVSEKAPQSLESVLSTMLTNVTQNPVQSLIDANYLGVLFWAVLLGLAFRARSEST
                     KELVDQISIALSQVVQVIIAFAPIGILGLVYQSIATTGIAGLAEYLQLLLVLIGTMLF
                     VALVVYPFLTFLFIKENPYPLIFFCLKESALPAFFTRSSAANIPINMMLAERLKLTKE
                     SYSISIPLGATINMGGAAITISLMTLTAVHSLGIEAPFVLKVLLCILSALAACGASGV
                     AGGSLLLIPLACSLFGISNDVAMQIVGIGFIIGVIQDSMETALNSSSDLLFTAIGELG
                     ARKRSGEKIKLKDCLDGLSER"
     gene            77301..78299
                     /locus_tag="IWO89_000068"
     CDS             77301..78299
                     /locus_tag="IWO89_000068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0454862.1"
                     /translation="MFLSNFFVRCLFLKKIKKESYGFLTIFVACIMMVLQRILISAVT
                     IPTYATNPGRFYIYTFLQTSVVIGANLIPLYMGYQYQKIARLKVLHYLSRFAFIFLIA
                     TLVCNIFFYVQAGSLNIRDYWLILTPISQNYFTYAVSCTLLFCSIPYTVGFLNKLSKT
                     TIKYLLLFLTIGLVGCSTLFNKDPFALQNGQSILWIFYLFLMGYGLKEWNWKEKVRYK
                     LPQVFISLIVLFGLIILMTQISLIIRGNTDTALRFSKPYSLFAMYYTFSSFLFLEMLS
                     DKLGIKGRGNFLAVFLIVTQVICNFPLVSYRVSTFMKREFPNSGKAWLINIGQFFI"
     gene            78312..80330
                     /locus_tag="IWO89_000069"
     CDS             78312..80330
                     /locus_tag="IWO89_000069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HAQ0454863.1"
                     /translation="MSLLVIFLFYIQKIRFIHRFIEYFAFDSIEQLTQRWKKRIHWLF
                     VHKSYFLVAAFFYCFTFVQIFVLEPKEGWKETIQVALKIFTQRQAPMILTIIIIMGFF
                     FLLLLISNRFWYALTATLIINLLLTISTVIKMEMREEPVFPSDLKMLTGLSELLSMVS
                     PVLLIVGGLILLLLLITSIIVQRRLQKQYSLKIHWKRRFISIAVLLGCFSGVFFINHK
                     NSPSFLLFNLFKVNKTFFNQQGAVKDNGPVIQFLNNIDIKIMEEPAGYSKEKINGIMK
                     KYNTKADEINQTRSDWLNNQTIVLNLSESFSDPARVPNLTVPTDPIPYIKSIITEGTG
                     GLMLSVGYGGGTANMEWEGLTGLSISNLSASLVTPYTQLVERQKISPNITDLFDEKIA
                     IHPFTAALYRRKQVFDKFGFDRFYYLDSPDKLTYTDKIEQSPRISDDSAYKETLKALQ
                     SNTETSQFIQLSTMQNHMPYDDYYSELNYTAEGTAVIPNRRKELQTYMQGLHYTDTAV
                     KKFIQEIDKIDKPITFVFYGDHLPAIYSGNDSKKYGLEQHQTDYFIYNNKYSREQAAT
                     LNKEVVAPNNFGAMALEHANIKVTPYYALLTEVSNHLPALTIDPTESLSNRFNGKQVF
                     VTEDSQIIQEKNLTEKQKEILEDYRLIQYDLVAGNQYSATWATQKIKD"
     gene            complement(80390..81106)
                     /locus_tag="IWO89_000070"
     CDS             complement(80390..81106)
                     /locus_tag="IWO89_000070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4767 domain-containing protein"
                     /protein_id="HAQ0454864.1"
                     /translation="MKKRILVTIGLAFLLAGCTNGNESKKESTKETAKATVATTTTTS
                     TESKSTAASTETADTSTSSESSKTTETTQTSTSSSSVEASSSSSESSEQVEQTLWNAD
                     KSAQLEAFMTKWGQTMGQQYKSYTNQMSVDLYGLKVPQAILNGEWKMAIGGVPVSAEW
                     SESGTGQADYQITAVYSDAETEPYLKKHVYLFGFQQNQPKVLVTQQNQGNPDNYLYFN
                     ETANNELKNGFNQIVYGQAN"
     gene            complement(81333..81875)
                     /locus_tag="IWO89_000071"
     CDS             complement(81333..81875)
                     /locus_tag="IWO89_000071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HAQ0454865.1"
                     /translation="MNIVEEKFVQETKQADIDHHKPTAGAMVGHVLANFKLEEMKLIQ
                     AQLYVLGTSSQELSTLFSEYADQEARWFNKISHELVIENEIVPSTLEEVMRYGKIYEH
                     GQNKYLPASTMLENFAKDFDFQNVFITRAIKLAEKEEKYGLQQLLVELLSFNKEIIYQ
                     VQRLQGKTVRQDLDEEDDDD"
     gene            complement(81880..82107)
                     /locus_tag="IWO89_000072"
     CDS             complement(81880..82107)
                     /locus_tag="IWO89_000072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper resistance protein CopZ"
                     /protein_id="HAQ0454866.1"
                     /translation="MKKIIMQLDTLACPSCMQKIEHAVSQQPGVSNTKVLFNASKIKA
                     NFDPDEVHPEQLAAVVEKLGYEVQSAKVKEA"
     gene            complement(82186..84066)
                     /locus_tag="IWO89_000073"
     CDS             complement(82186..84066)
                     /locus_tag="IWO89_000073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HAQ0454867.1"
                     /translation="MKLQKWIQEHRNHITALSAVLIIFGFIGKFTGFVLFYNIALAIA
                     SIIAVIPIMIHAYQAVKVKVISIDLLVTIAVLGAFMIGEYKESAIVTFLFLFGSYLEQ
                     KTLTKTRESIKALTEMAPSTASVVQEDGTTEEVEIDDVEEGDIVLVKTGGAIPVDGRV
                     IDGKGYADEAAVTGESRQVVKISGDEVFSGTILADGYVTIEATKVGDDTTFAKIIELV
                     EEAQDTKSHAEKFIDRFSQYYTPTVLLIALVVGLISRDFRLAITVLVLGCPGALVIGA
                     PVSNVAGIGNGAKRGILVKGGEAMDTFSKIDTFVFDKTGTLTKGTTAVSTIKNYTEDE
                     GDSLALVARLETLSDHPLGRAVIHYAQEKDIDFQQLAVKDNQTIKGQGITAEIDGHTV
                     CAGNTKLIAAHKLSLTPKQVQDLHQLQQTGSSVIIVAIDKKITQIIGVSDVIRPEVAE
                     QLAKLKQAGAKQLVMLTGDNQMTADYVADMLGIDEVHAELLPDEKVQFVKKYQEQGLR
                     VAFIGDGINDSPSLAAADIGIAMGSGTDVAIETSDVVLMQSNFESLVHAYRLAKKTVL
                     NTRENVVIAIGVVLFLLNGLFAGFIYMASGMFVHEASILIVIFNAMRLLYFGHESKEE
                     KS"
     gene            complement(84255..84869)
                     /locus_tag="IWO89_000074"
     CDS             complement(84255..84869)
                     /locus_tag="IWO89_000074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAQ0454868.1"
                     /translation="MKLTFKPLEKKDYKKAIYFAVEGMNFERYMENELLLNLYGRYFL
                     YMELNRATQILALYAEDELAGVLLAEIKGEKKKHRSFGQQIYVKIFDFLQNIFAKESK
                     GAYETANEQMLAAYLKKHSPDGEIIFLAANPDLKTKGIGTKLLTEFEKREKGKEIFLF
                     TDSGCTYQFYERRGFERLKEKISKIKILDKEIDLRCMLYRKIIQ"
     gene            85011..85571
                     /locus_tag="IWO89_000075"
     CDS             85011..85571
                     /locus_tag="IWO89_000075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HAQ0454869.1"
                     /translation="MKKTDRRVKKTEKALAETLSTLLVNKKIQAITIRELTETADVHR
                     STFYTHYKDIYDLYDQLESNFFRDLNEILSYDPAHSYEELYTRLIDYVYANTFLYRLF
                     ILNHGDISFQKKVKQVIEENYLNIWLYEDKKTIITEEMRFFTTYHVQGCLSVLNRWVE
                     SNYKYSKKELLELLQKLNDHIEKIMP"
     gene            complement(85876..86190)
                     /locus_tag="IWO89_000076"
                     /pseudo
     CDS             complement(85876..86190)
                     /locus_tag="IWO89_000076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334404.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction endonuclease subunit R"
     gene            complement(86444..88079)
                     /locus_tag="IWO89_000077"
                     /pseudo
     CDS             complement(86444..88079)
                     /locus_tag="IWO89_000077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294751.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(88634..89197)
                     /locus_tag="IWO89_000078"
     CDS             complement(88634..89197)
                     /locus_tag="IWO89_000078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HAQ0454870.1"
                     /translation="MITVVHKERQEKSSSDVRLWVYLGSISTKIQYGYTDSAKKQGNV
                     KFLRITDIQEGRVNWSSVPYCDISNSKLVDLRLEENDILIARTGGTMGKSFLVKEISE
                     ESVFASYLIRIRLVEKLLSEYVDCFLDSPLYWKLLEKISYGTGQPNVNGTNLSKLLIP
                     LPPLEEQQRMTTKIEMIRRSIRRINFE"
     gene            complement(89212..90255)
                     /locus_tag="IWO89_000079"
     CDS             complement(89212..90255)
                     /locus_tag="IWO89_000079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0454871.1"
                     /translation="MTQRVAIMILVLLVIGLLVYYVLKFKHWKQQRIHQDIEKKLKRY
                     PIVQAAWEKAEAKQYNIPGLTETRMVVPETGENEVCQWMTPQGLAFSQDFVFISAYCY
                     DHQHHSIIHVLDRETGQPIKLLILPKRPHVGGLVYDTKRELLWLTITGSATGRVAALR
                     LIDILADTSEETGQPIAYWLTTDLSEIPQASYLTQNNDQLVSGNFTLKGEGQLTFYLL
                     PTIAEMKTAIRRKDKIQPTVTMSRKTSDKIQGVAFYGHYLLASRSYGPYTSELLVSER
                     DSPSRILKRIKFPPYLEQIVVVEDRLAVLFESGAAAYREKANPVLANVLLLDLATLLH
                     ANKRPKKIAATKK"
     gene            complement(90388..90792)
                     /locus_tag="IWO89_000080"
     CDS             complement(90388..90792)
                     /locus_tag="IWO89_000080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="organic hydroperoxide resistance protein"
                     /protein_id="HAQ0454872.1"
                     /translation="MKKMYETAMINRGGRDGEVEAPNGSMHMKIDRPGIHSEGTNPEQ
                     LFAAGYASCFNGAVQHMLEENNLESDSEVKARVSLFQLEDGGYQIGVVLEVSLPGLEK
                     DQAEKIAEEAHAFCPYSKATRGNIDVEVTIVE"
     gene            complement(91025..91660)
                     /locus_tag="IWO89_000081"
     CDS             complement(91025..91660)
                     /locus_tag="IWO89_000081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF998 domain-containing protein"
                     /protein_id="HAQ0454873.1"
                     /translation="MIFLQKYGFYFLLLGVMSDLSTPYILGLFYPKLNQMTTVISVFG
                     DVDSPVRRAFLVWSVVSGLFFVLSLPALYHLFVGTSKTLAILVVATVGLYGIGDCIFT
                     GLFSINTNESSCNLSTWIHNIGSGLGYAGFLLFPLLLVLLYRQSGQGTLSHFYLVLTV
                     ISLLIAGLYGLARIPSISQFAFFKQLGFFQRLSFFFNYLPMICLSFFQLRK"
     gene            complement(91926..92978)
                     /locus_tag="IWO89_000082"
     CDS             complement(91926..92978)
                     /locus_tag="IWO89_000082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAQ0454874.1"
                     /translation="METSQKISPKIFLNKVLAGTATGIIIGLIPNAVLGAILKYFSAY
                     PFAVTIAQLAVIFQLATPLIIGGLIALQFGFDPMRMMVVAGASFVGSGVVKFTPDLGE
                     TGVYVGAGTGDIINTMLTASIAVGLILLIGNSFGSVSIVLTPIVVGVGAGLLGFYLFP
                     FVTAITSAIGSLINNFTTLQPLLMSILIGCSFAFLIISPISTVAIGMAIQLDGVSAGA
                     AAMGVAATTFVLVVNSWKVNKSGVTIAIALGAMKMMMPNLFRKPVILIPCLVSAVITA
                     IPVALFSISGTPASAGFGVVGLVGPLASLDAGLNGFLVVLCWLVIPVFAALITQFLCE
                     KVFKLYDREEVFKFLG"
     gene            complement(92991..93929)
                     /locus_tag="IWO89_000083"
     CDS             complement(92991..93929)
                     /locus_tag="IWO89_000083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015081805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HAQ0454875.1"
                     /translation="MKIAIAGAGAMGSRFGLMLHQSGNEVLLIDGWAEHVQQIKEHGL
                     QANFNGKEVEAKLPIVLQSEVEKEDQVDLIILFTKAMQLEKMLQDIQSLIKKDTEVLC
                     LLNGIGHEDIIEKFVPMENIYIGNTMWTAGLEGPGQVKLFGSGSVELQNLGDGKEAAA
                     KKLADKLSESGLNAHFSDNIHYSIYRKACVNGTMNGLCTILDVNMAELGKTSTAHKMV
                     ATIVNEFAKVAAVEKIELDVPEVIAHCESCFDPETIGLHYPSMYQDLIKNHRLTEIDY
                     INGAISRKGKKYGVATPYCDFLTELVHAKEDSLNVK"
     gene            complement(94380..95030)
                     /locus_tag="IWO89_000084"
     CDS             complement(94380..95030)
                     /locus_tag="IWO89_000084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0454876.1"
                     /translation="MNDEYKNDEDKMLFEEIENRCRLNFELRGKMSLIQQKKYLANKS
                     EFTLGHVEKLISDWISSRSEFTKIKQPIKFDMKKLLLNKSEIGNRDQYIRAKGQEIID
                     SLGEMRSYNYLYVTHRADGMVITVGKSSSNDIFLDGDLFYQLNINHLSGTENIILRTE
                     YGNEIFAKYDEILKNYLDWAWIIPVESGDAKKLERLLGDELINKKVPILNYYSHRQ"
     gene            complement(95130..95639)
                     /locus_tag="IWO89_000085"
     CDS             complement(95130..95639)
                     /locus_tag="IWO89_000085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1456 family protein"
                     /protein_id="HAQ0454877.1"
                     /translation="MNNNDRLLRLRYAIDLKDTDLIKAFELGGVPLTKEEAQAVLTKV
                     QDKNKDNAENNVYEKTINNQVFDAFLNGLILLARGPQKDKPIVDTPQKSKEIKYINNV
                     LLKKLKIALSMTTDDILDVFAEAEIYPSKGEIGAFLRKEGQRNFKPCGDKYMRNFLKG
                     LGIYNRRKV"
     gene            complement(95840..96709)
                     /locus_tag="IWO89_000086"
     CDS             complement(95840..96709)
                     /locus_tag="IWO89_000086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HAQ0454878.1"
                     /translation="MYGSIEAGGTKFVCAIGNDDLKVLERVSFPTTTPNETMSLVIDF
                     FNHYKEQLESIGVGSFGPIDIHRESKTYGHITSTPKTAWKNFDFVGTLNKHFEIPIAW
                     TTDVNAACYGEYVSGHGKGLSSVVYYTVGTGIGGGAIQDGIFVEGFSHPEMGHTLVKR
                     HSGDTFSGTCPFHHDCLEGLASGPAIEMRTGTKGQDLSIEDPFWEIEADYIAQCACNT
                     TLMLSPDIIIFGGGVMQQEHLKKKVQRRFLELINGYVDTPNIEEYIVTPKLADNAGTI
                     GCLTLAKDVRINS"
     gene            complement(96729..97850)
                     /locus_tag="IWO89_000087"
     CDS             complement(96729..97850)
                     /locus_tag="IWO89_000087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2961 domain-containing protein"
                     /protein_id="HAQ0454879.1"
                     /translation="MNCLNNIWLHKGEESRTISPENKNGEKGKAAMTASHLGIGRKGS
                     PSVPINAGETITLAEMEGPGILRHFWMTVTDKTIAGSFVLRDLILRIYWDDESTPSVE
                     SPLGDFFCNGFGTRCEVNSLPIVVNPTSGMNCYFEMPFRKKAKITLTNEHPEFIKHIF
                     YTFNYALTEVPDDAMYFHAKWNREPITTKGKDYTLIDGIKGTGYYVGTYFALCALERY
                     WWGEGEFKFYIDGDEDFPTITSTGAEDYFGGAWAFHKKNFGERAYAQNFNTLFMGYPF
                     QSKRDHTRDYFSAGKPNPNPVHGFGDDALPMHGLYRWHLPDPISFKENLRVTFQTLGN
                     DDIKLYERQDDISSVAYWYQKEPHEFFEKLLDREERIPR"
     gene            complement(97852..98247)
                     /locus_tag="IWO89_000088"
     CDS             complement(97852..98247)
                     /locus_tag="IWO89_000088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIA"
                     /protein_id="HAQ0454880.1"
                     /translation="MRRLIIASHSILAEGFKETLEFLTGMHNQISSLCAYVEEIDDID
                     EQIKELVDTTDEIIVLTDMAGGSVNQKFFTYISDRVHVIAGMNLPLAMSIALQLGNDN
                     LDIGAIIAEAQQQIIYMNNQKSEERIEDE"
     gene            complement(98266..99063)
                     /locus_tag="IWO89_000089"
     CDS             complement(98266..99063)
                     /locus_tag="IWO89_000089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010238514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HAQ0454881.1"
                     /translation="MSEKYQVTKKDLRKANYRWLMSVCTFNYQTQQGASVTYALSPIL
                     RKLYKDDDEYIEALNNHFQYYNTQPWLAAIILGACVAMEEKQGLEAKDAINDFKVGTM
                     GPIAGIGDSLLMTMIPTIMGSIAAYMALENNPVGIFLWFILIMVIFFFRMRGFEFGYK
                     QGMKIVTEYGEKINYLTEAASVLGLTVVGSLIASVISVSTPLKFTFGEVSMKIQPMLD
                     KILPTLIPVIITAAAYYLLSKKKISMTILILLVIVFAMVMAAFGILA"
     gene            complement(99065..99853)
                     /locus_tag="IWO89_000090"
     CDS             complement(99065..99853)
                     /locus_tag="IWO89_000090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAQ0454882.1"
                     /translation="MHFTIFQIIMLTILAFIKHVDYYGIPMIFVNYAVFWGLFTGILM
                     GDIKTGLIVGGTVQLMSLGVAGFGGSSVPDYGTCAIIATAFGSQLGVEAGLAIGLPVG
                     MLGVQLDVIVKILNGFVVRKSQEYANKREFKKMNSVLWLGPIFFGLSAAIPVFIAVTL
                     GQSAVNYVLDVLPNWFMDGLTLAGKMLPAVGIAMLLRFMPTGRYISYLLVGFFLAAFL
                     KVPIIGAAIIGFALAFNVYHRAEKDAQITASAGASDVGFIGEDE"
     gene            complement(99867..100343)
                     /locus_tag="IWO89_000091"
     CDS             complement(99867..100343)
                     /locus_tag="IWO89_000091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAQ0454883.1"
                     /translation="MSIVMSRVDNRLLHGIIVTQWAPMSKANRVMVIDDSVANNDIQK
                     SSMRLARPAGMAVSIITEETAIANFKKGKYDQEKVFVIVKEPETILHLVDNGIRIDRL
                     IVGGTVVKDNALQLSSRAYASDENISTYKKLLDSGVEIVTQFVPADKAQEIQSKLG"
     gene            complement(100415..103072)
                     /locus_tag="IWO89_000092"
     CDS             complement(100415..103072)
                     /locus_tag="IWO89_000092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HAQ0454884.1"
                     /translation="MEELKERIVNYVIENSEIKNKWGNNTAQKISEKLNVSRNIVSQY
                     LNEFVKDGEFGKINTRPVIFFSLPEKVPNQQIFSSSEEAEKWYDSSFRVGIERVIGSQ
                     GSLRNVIAQIKAAIYYPPNGLPILLHGETGTGKSYLANYVYKDLLANNYIEEKASFIS
                     VNCSEYANNPELFLSNFFGHVKGAYTGAEDGRKGLAELANNGVLFLDEIHALSNECQE
                     KLFQFMDTRKFHRMGDNENWYTSSCWIIFATSEPPEDKLLSTLLRRIPVKLTLPTLDS
                     RSLYERKQLIKIFFKSEEERLAKKILISQQLLDFLLNYRFSENVGNLKNIIQLTCANA
                     FYFLDDLEELYIQMYHLPQWVNFNGMKVMKRQNELKYIQMDDLEKVDEVFNINELIEY
                     LYQTWKKDTDKQQLFEKYDRYFRQKNVDIQNNSALTYPTYQEIRFRQYVKEIERDFQI
                     EFDYYYLQITSIFLNSWIYQIQLTSDDKLYSFINDINSWCSREVQLVNRFIQIGDIYL
                     TKIQKNVLVILFKLMCSKEFQQKRLALILAHGDLTATCIASTANRIVERYIYDAIDMP
                     LMTSSSEIADKINRYLQECTGYEEIILLVDMGSLEKIYLNLELDAQKKFGLLNNVSTR
                     MAIDVANQILLDLPLKQILEQVKENQNVDYIYESSNIKKDMIVCSCASGLGTSFKLKK
                     ILEDSFPRDTDLVIETCDYYSLVNENYLSELRKENNILFIVGTLNPHLEDVEFFSIED
                     MILGMNISKFSNQLNRHFNDNELKVLNNNILKNFSLTNLMEQLTILNPTKLLERVSDA
                     IYILQNDLGISFDNNTCFGLYVHISCLIERLVKQNTLEDEIYFNETSEEFQKFQTHFK
                     QSFSVVEHYYSVDIPIHEVKYVYDYVKRA"
     gene            complement(103091..103495)
                     /locus_tag="IWO89_000093"
     CDS             complement(103091..103495)
                     /locus_tag="IWO89_000093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit M"
                     /protein_id="HAQ0454885.1"
                     /translation="MGAELNQKLFSAADNLRSKMDASEYKNYLLGLIFYKYLSDRLLE
                     QVVLLADESLEEYDTVSKQTMLYRELLSDEESKEDLIATIVDILGYAISPEYLFNVLA
                     DQAKQATFQLNDLNKAFLASHIYGKILSAKFE"
     gene            complement(103727..103984)
                     /locus_tag="IWO89_000094"
     CDS             complement(103727..103984)
                     /locus_tag="IWO89_000094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP pyrophosphokinase"
                     /protein_id="HAQ0454886.1"
                     /translation="MEDSPELFPMLKDRLNELTEKRRQLQIRENEILGIFQQQGEPIQ
                     VKDVQQILTSLDCFLIQSEKKQVKALYHTFIEKITFDPNQK"
     gene            complement(104109..104441)
                     /locus_tag="IWO89_000095"
     CDS             complement(104109..104441)
                     /locus_tag="IWO89_000095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0454887.1"
                     /translation="MPLDLSTGRKIRFSEIQYEGIGFTLIDLKNSTFEIKEGLDLKNL
                     LCADDKIKKIQLYKVPILTPTVLTMLEMENNINYIDSGSDLLIRWQVITNFLMELKKY
                     SNTQTKFS"
BASE COUNT        31612 a        21632 c        16991 g        34607 t
ORIGIN      
        1 aaaaatagct gctcatattt tgccgttttt tgagctaata ccgaaggtca gctgattgaa
       61 caccgtttac caagaggttg tgagcctacc atttaactcc gaatcacaag aaataatttc
      121 aaaaacaact tctcctgttt ttggtttggt tggcttatta tcgagaagta gctactggga
      181 gtctatttat caaaaaaagg aatgcgacat aggtcacacc ccttttttat tttctttgaa
      241 acatctttcg gtactcgaca gcatacaaga caaaagattg taaaagaccg taagtgatga
      301 cggcaaaaat gactctcacc cacagcgttg gtaattctgg gaaaagcagc aaaaggatca
      361 tgccaacata gatgacagca gtcgctactt tgccatacca cttcgctcca cttaatttga
      421 tatttttaga tagcccgatt aatccagcta caaacataaa gccttctttt atcaagaata
      481 gaccaaacaa cagccaaagc cacggtcgat accatgccat agacaaaatc agaaaaagtt
      541 gtgtcaattt atctgcaaac ggatcaagca ccttccctaa gtctgttacc tgattatatc
      601 tccgagcaat gaccccgtct agataatcag acaaacctga gacaacaatg accgctcctg
      661 ctatataaaa tgactgtctc agcacaaaat aaagataaat cggcagcaaa aaaatacgga
      721 atatcgataa aagattcgga atcgtttgta tttctttacg ccagtctgtg cttaatttca
      781 ttttatcgcc ctacctcctc agcactcttt tctttttatt ttagcttaca cggggaataa
      841 aaccaatgat ttcactgttt ttatttttct ttacagccac tgatattatg agctaatttg
      901 agataatcac ttgctgtttt catcgtttac tcttctttct tcctgtcgtt tgcgcatttc
      961 ttttactagt actttatgag ctttttttga tcctcgctct aggtcacgct gccgtttacg
     1021 tgttttataa tcgataaaca gactgtctaa atcgtaacct gtcgggtaca gatcttctgc
     1081 tgaacgcacc agacgaacac gctgacgagg cacgtggatt atttttttgt ctaaatatac
     1141 atcaatcgta tcttcaccta tatcttcata aactaacgct tctttttggt attcattgac
     1201 aaatatccga tcaccttttg caaagagctg gcgttctttt ttattctcct tcctatttgt
     1261 aactgctgtc ttaggaagaa attctttttt ctgtgtccga taggcttttt ccgccaagta
     1321 acgctctgct tgatggatca atttcatcga catatccatt ttatgagcaa tccacaaagc
     1381 ttggctttct cctgtttctc caacccgcaa ctgatattta ggacgaagtg cttcccgatc
     1441 gaaagccata gcagccggca caaaatcttc atgatctccg gcgaatttct tgatttctcc
     1501 ataatgggtc gtggcaatga tcaatgcccc ttgctcatac atagattcca tgattgcgat
     1561 cgctagtgca gccccttcat ttggctctgt tccactgcca atctcatcta aaagtaccaa
     1621 cgtattcctt tttatctttt tcaaaatagc agcgatgttt tgcatatgac cagaaaatgt
     1681 actcaacgca ttttctaaat tttggtggtc accgacatcg acgaaaatct catcaaagac
     1741 agcaagctct gtcccttctt ttgccggaac ttgcataccg aacatcgcca tcaaagtcaa
     1801 taaaccgact gtcttcaaca cgactgtttt gcctccggca tttgctccgg tgataatcaa
     1861 gcctcgatag tctttaccga tttcaacatc caatggtacc gcatgctcag gtaaaaatgg
     1921 atgtctgcct tgcttgataa tgatatgttc cgatttattg attctaggtg tgcttccatt
     1981 gatttctcgg ctaaattttg cccgagcaaa gatgatatct aacaccgtga tcgtttcgat
     2041 caataaatcg atcacttctt cgttttctgc gatagcacca gtcaatgccg ccaggacttg
     2101 atattcttca ctgatttctt cagcttttaa caactgatag tgttcattca atttagaaat
     2161 cacagttggt tcaataaaaa ccgtcgttct tttatttgat tcatcgataa tcgttccatc
     2221 tattttattt ttatagctgg ctttgatagg aatcgtatag tattcgcctt tttgaacaat
     2281 cattgcttct tggatcattt ctttgttttt cggatgacgc aagaatttca gtaattttga
     2341 ctggatttct ttttcgatta gttgtaattg tttgcgtact ttacgcaaat tacgtgacgc
     2401 gtcgtcactg actttattat tttttatttt ctggtcaatc aactcttcta catttatcaa
     2461 atcaggaaga tgtttgctat aagcaaacaa caagggtgtc tggtattgat tcttatcaaa
     2521 aaactttgtg atcatccgac tgcttctaag aaaatccgca tattcgatca aatcggttgg
     2581 ctgcagaatc aacccttttt tgacttgttc tgtcagtgca tcgatcctcg gcaaccccat
     2641 aaatggaaca tgctgactgc tttctaagat caagcgtgct tctcttgttt cttcttgacg
     2701 atccaagaca gtttgtaggt ttgtcgagac cgtcatatcg ctgattcgtt ttttgctata
     2761 gtttccaatt gctcttgctt ggatttcctt tttgatccga tcaaattgta attgactaat
     2821 catttcgttg tccatattct ctccttttct tgaagagata cctgtcccaa aataaataag
     2881 ggcctagaga taaataaaag gatagaacac agcttaaaaa agtcatgtcc tatccttatt
     2941 agaatacaaa gatattgttt tcctgacagg taccgtttca taagcacacc aaataaactt
     3001 tgtttattgg ctattcgact tactgattag ataccagcta cactcacaaa acatacccca
     3061 ttcttctagg tcttatttat tttccactta actatagcaa gacacatttt ggttgtcaaa
     3121 ctttagtatg ttttatagat ttacaccatt aatgaataga cacctaccgc gataattgct
     3181 ccgatcattg gacctgcgat tggtacccat gaatatgccc aatctgaagt tcctttatta
     3241 gctattggca ataccgcatg tgctaatcga ggaccaaagt cccgagcagg gttgattgca
     3301 tatcctgttg gccctcctaa tgaaagaccg atggctaaga tcaatccacc tacagcgaat
     3361 acgttcgttc caggtgcaaa ttcgttttgt ccaaaagcta gcaacccaag aactaataca
     3421 aatgtaccaa ttaattctgt gatcacattt gctggataat tccgtatagc aggacctgtt
     3481 gcaaacgttc ctaaaatcgc gctttcatct ttcgttgcat tccaatgtgg caaataagaa
     3541 agccaaacaa gaatggcccc tgcaaaagct cctaaaactt gcgcaatgat gaaaggaacg
     3601 acaagattcc aactaattgc ccctgtcatc gccatagcta gagagactgc aggattcaaa
     3661 tgagctgggc ccatgaatcc agaaatatag acagccattg ttacagcgag tccccaacca
     3721 aatgcgatga caatccagcc agaagcctgt gctttacttt tgtttaaatt cacagccgca
     3781 cacacaccat ctccaagcaa aactaggatc agtgtcccaa taaactctcc catgatttga
     3841 gtcatatcac tgttcattta tttctccccc tttagttccc gcagatctga ttctgcaatc
     3901 aatgcttcta aacgtttagt ctgttgcgcc ttctcctctt ctgtccattc aaaatatcca
     3961 gcaattgcat cgatcacagg ttgcttgatc tcatcaagct gatctcgttc aaacaacaga
     4021 tgatttgtac gacgaatgag ataatcgcct ggggccaaaa ccatttcttc ttctaaacca
     4081 taacgtaacc gagctgactc agcgagactc aaaccaggat aaggtgccat ttcttttgct
     4141 aattcaaaga tacgtcgggc attagtcccg taaaaatcag cgatataagt agcatcttct
     4201 tcctctaaac cggctgctac tccttctttt gctaattctg tcactgtttc ttctaattta
     4261 gttggatcga agtttccacc tgaaatctga tattttttag agtcgatttc tttcgtctct
     4321 attccgtatt cttcttttaa taattggcgt atcaatcgca aagctccttc tgccatctta
     4381 cggtaatcgg tgattttccc accagataaa gtcagcaaac catctggttc tctttctaaa
     4441 gaactacctc tggaaatcgt agaaggtgct ttttcatcac ggctgttttc caaatggttc
     4501 aacacgtctt ctacttcagc acgagaaact ttgttttcct tatattcact tactgtatcg
     4561 acaactttat tgaagctctt gtctgaaatc gatccattat ctccaccatt gtaatcagaa
     4621 ccagaattac caatcaacag tggacgaagt cctgcccagc ttgcttcgat atcattcaat
     4681 gtgatatttg cttctggata gcgatgattg atcacatcca atagataatc tacatcttct
     4741 tgtgtgactt tcggatccgt aaaatctcct tggtaatccg tatctgtcgt accaaagtaa
     4801 gtcttgtttt ctcttggaat agcaaaaacc atccgcccat cttgctttcc tgtatcgaag
     4861 tatgtgggtt gcggtacagg cagttttttc gcatctacaa ctaaatggat ccctttagtt
     4921 ggacgcattt taggagagac tggacgcgta aaattcaagt ttcgtacttt atctacccaa
     4981 ggaccactcg tattgatcac taattttgct ttgatttcga tcacttcatc tgtcagcagg
     5041 tcacgagctt tgacgccaac gatttgatcg ccatcatata aaaagccaac cgctttcatt
     5101 ttacttacta gataagcccc atcttcggca gcctttttga tattatcaat cactaaacgg
     5161 gcatcattgt tgcggaaatc tagatagaca cccgcgcctt ttagcccttc ttttttcaaa
     5221 aacggttctc tttccaatac ttcttctggt gtcagcgtat aattctcata tttggttcct
     5281 gtcacatttg ccagtttgtc ataaaggtcc attgctactt tgaccgagaa catattgaag
     5341 gttgttgctc cttcgccctc atagattggc aaaagcattg gatctggttt tgggatgtgt
     5401 ggggcaatac tttgtacgac tgcacgttca ccaactgtgt cagctactac ttccacatca
     5461 aatgtcttca gataacgaat accgccatga acaagtttgg tcgagcgaga ggaagtccct
     5521 tctgcaaaat cctgcatttc gatcaatcct gttttgattc ctgatgctgc tgactggact
     5581 gccacaccgg caccagtgat tccgccaccg atgatcaaca gatccaattc ttcttgctgc
     5641 attttttgaa tgctatcttg tcttgtcttg tttgaaaaca tatggatttc ctccttattc
     5701 aaatgcttgg gtagctttta ctgctttttt ccaaccgttg taaagttttt ctcggcgctc
     5761 ttcctccatt tgaacttcaa atagcttgcc agcttcataa aattcgcgga tctcgtctgt
     5821 atctttccaa taaccgacag ctaatcctgc taaaaatgct gcccctagcg ccgttgtttc
     5881 caagttttct gctcgttgga tcggtacatt caagatatct gtttggaatt gcatcagata
     5941 ttcattgttt gctgcaccgc catctacttt caatacagga atcttgattc cagtgtcatc
     6001 ttgcatcgta tcgatgatat ctctcacttg ataagcaatc gactgtaaag tagctttaat
     6061 gatatcttct tttgtcgtac cacgtgtcaa gccaaacata gcccctcgtg ctgcctgatc
     6121 ccaataagga gcacctagcc caacgaatgc aggaacaaca taaacctcat cttcgcttgt
     6181 agagcgtttc gctgcgtctt ctgaatcgct agctttttgc aacatttgaa gtccatctct
     6241 cagccattga atcgaagaac cagccacaaa aatacttcct tctaaagcat agtaaacctt
     6301 tccatttatg ccgtaaccaa ttgtcgtcaa taaattattt ttggataatt gcggtttttc
     6361 accggtattc atgacaataa atgaacctgt accataagta ttcttgacca tgcctggttc
     6421 gaatgccatt tgtccgaata atgcggcttg ctggtctcct gccatccctg aaattggtgc
     6481 ttcgcttcca taaaaatgat aaccttgcgt ataaccataa acttctgagt tagaagtgac
     6541 ttttggtaac atcactcgag gaatatttag taaatctaaa atctcctgat cccaatctag
     6601 ctgatggata ttgaacaaca tcgtccgact ggcattcgag taatcagtaa catgtgcttg
     6661 tccatcggtc agtttccata ctaaccaaga atcgatcgtt ccaaacatca attcgccttt
     6721 ttcagctcgt tcttgcgcgc cttctacatg atcaagaatc cagcgtatct tcgttgctga
     6781 gaaataggca tcgatcacta accctgtttt ttcgtggaaa aattcttgat agccttcatt
     6841 ttttaagtta tctgcaattc cagctgactg tctggattgc caaacaactg catgatagat
     6901 tggacgtcct gtatcttttt cccatattac tgttgtttca cgctggttcg ttatgccaat
     6961 cccagcaatc tgatttggtt tgatccctga ttcgatgaac gctccggcaa taactgactg
     7021 tacagaattc cagatttcat ttgcatcatg ttccacccaa ctttcttgcg ggaaatattg
     7081 cgtgaactct ttttgagaac tgccgatgtg tcttcctttc ttgtcaaaga taattgctcg
     7141 tgagctagtt gtaccttggt caatcgccat gatatacgtt gattctgtca ttgtgattcc
     7201 ctccattata tgcaaacgct ttctttaact actcttatca tactactgtt ttaaaaaaag
     7261 aaactgtcaa aattttattc tttttttctt tttttgacag ttttttatta tgaagctatt
     7321 ttttaatcaa ttcttttctt ttttcagcgt ctctattctt cttttcaatc gaataatgtc
     7381 gtaaggtgac gcaaattctg acagataata ttcacactgt tttttgtttc tctggcaaaa
     7441 tgtgatcacc aaatcatatt ctttaccttc ttcataactt ccaatctcaa taccgccgat
     7501 tcccctcagt tctctgatca gatattgttg aaaagcaata cgataaatcg gcagactttc
     7561 taaatcgatt ccaatcacta ctggttttgc aatataaaaa tcgatcatca aaagaacatt
     7621 ggcatactgg atcatcaggt aactaagctc actatcttca gctcttttcc tcggcaattg
     7681 ctgcaccgct gtttcctgta atttcgccag taactgtgtt aaggaatgcc cgagcatttt
     7741 ctgctgctgt tccaaaagcc gatcacgctc atagacttcg atctttccaa caaagaaaaa
     7801 ccattcattg ttgatctggg aaatttggta gcctactgcc ttttcttctt tgattttcaa
     7861 acgacgagga cgataatgcc ataacatcgt ttcccgaatg taagtatcta aaacagcggt
     7921 tggtaacttt ttgctccgtg tcaaatcata ctgataaaat atttcttcgt ctactatcga
     7981 aaaagaaaca aaaaagctgt aaaacatcat tgtttcgtat acattcagtt ctgctgctgt
     8041 tcgactaaga tgcatcacga tgattggatc gattgcttga taaagccggt ccgactgata
     8101 gagtaatttc ttttctttca aagttgcgta cgtacttttt tcattcaata gtcttttttt
     8161 cgtaatcccg agccagcaag caatctgtgc ttctgctgat gccgtaaacg ttgtttctaa
     8221 tacacggtcc aatcctagaa tgaacggtct tttttctggt gtattttgca aaaaaagcgg
     8281 tcgctgatct tccgggatat attggaatag ttcataataa aaatatcgta tctgtagctc
     8341 ttctccgtat aattgaccgt ttttgatttg taatccaaat tcctctaaga gctggttcaa
     8401 ttctttgatc tttcgaaaaa cagaagactc actgatatta aacgctgtcg ctaatcgaac
     8461 gatggatact tgctgatgtt ctaataaata gacgagcatc ttaaacttca agctttcctg
     8521 caataaaaac aatagaattt tttccaaact tacatcttcg tccaacacaa gcagcgcctg
     8581 atactcattt cgctgtactt ctactttttt ttccatcatt cggccaatca attcaatatc
     8641 ttccaaatat tgatcaaacg aggcttttga cagttggaca tgctctctca aaccattgat
     8701 agaggctgtt cccccttgca gaacaaggtt tcgcaaaatc aggatctgta actgctcttt
     8761 tctttctaat aatgcttcaa tcttcatagg cagtattcgc cacctttgac cacaccatat
     8821 ctggcacacg gtattctggc tggaaaaata gctcatgttc ttctaactgt tcaaacgtcc
     8881 atccttcttc tactgcaatt gtcagttggt caagaaggtg gaacatctct tttttgcttt
     8941 ggagctgtgc tccaagaatc tttttttcag aatcaaatag aaatttgagt ttaactttgc
     9001 tttgatttat tgctgtaagc ttgtagtcta cgtatcctgt ttcaattttt tggggataaa
     9061 aaatgctctc ttcttctgtc aaacctacgc ttcctaagaa atagccaaac cactgattgc
     9121 cgactggacg ataggttccc tgatcctttg tctgtgagcc actaattgtc ttgctgacat
     9181 ttcgtgctgt acggacagcg ttatttacta gagaaacata cattttttca tttgttggac
     9241 gaaaagttac ttggatcgca tcgccaatcg cataaacgtc tttttgagac gtttgcaagt
     9301 agtcatctac ccagatcgtt ccatcatcat tcaatgttaa ttgttcttcc cacatctgat
     9361 tgtcggggcg agaatgatta gccagcaaaa caagatcata gttctccttt cttttttccg
     9421 tctctacaat agcggtttca ttttcggtca aagacacaac ttgttcttct aaaaaaagtg
     9481 aaagcccttg atttttcatt tcctttacta acggttctgt catttcagga tctagataac
     9541 gaaaaagaat tgactttcgg ctttcgtaaa gatggatttg tttttttctt gatgccaacc
     9601 cttcagctat ttcgattcct gcttgtcctg caccaataat tgcaattttc tttgcattca
     9661 tgatcttttg ctgaagagcc gttacgtttt gtatcttttt aactgggtga ataagcgttg
     9721 attctgcaga aatatttcga aacttttgat ttgaaccagt cgctagaatc aaacgatcaa
     9781 aatccaattt ttttcctcct gctaaaacga cttttttttc tttcaatcct attactgtct
     9841 tttctgtgta aacatggatt cgatagcggt cagtcagttc ttctttcgtg atccaagaaa
     9901 gttgtttttc actggtaacc tttcctttta atatcaaatt gaacccgctg ggaatccatc
     9961 ctaacttcga ttgcttttca atcaaatata tttcacttgt tggcatcaat tggcgaagat
    10021 aaatagccgc atgaatccct gcaaacgacc cgccgacaat tacaatgttc atctgcattc
    10081 ctctttctta taaggtgttc tactgctaac ttctcaatca gaaaacacag tattcataac
    10141 tcgctttcaa ccttatgata catagcgatg cagcagaatc aaccctctcc atcataccgt
    10201 gttctaggtc tgcttctcaa taatgtacta gtggacctaa ttgaagagta tcatttctga
    10261 agagatgttt caaaaaatgt actcttataa aagaaaaaaa ctcgcctttt acgtacctct
    10321 gtccaaatag gaaacagaaa agaacgaaaa gacgagtttt agcgtgtgta atgtaatagt
    10381 tagaatagac acatcattta tttttaattc tttaatgctt caacaactgc ttcgcctaat
    10441 tgatgagcag attgaggatt ttgccctgta agaagccgcc catcaatttg aaccacttct
    10501 gaaaaagctg ctccagactg atagtttgct cctttttctt tcaaagcatc ttctgctaag
    10561 aatggtactt cattcgttgt accatttgct tcctcttctt cattactgaa cccagtcact
    10621 gtacgtccag ctaaaataga ttttccagac tcatctgtaa ctggcaacaa tcctacaaca
    10681 ccgtgacaaa cagctgagac cagcccttca tttcgataga tttgtgaagc gactttccca
    10741 agatctattg cttctggaaa atcccacatt gttccgtggc caccagcata ataaaccgct
    10801 gcataatcat ttgcatccac atcatcagga cgtaaggtat ttcccaaggc ccgatttctg
    10861 aaatcttcat cttcataata ggcccaatcg atttcatcca tattcaaaac actttgcggt
    10921 tctaaaggta catatccgcc tttaggactg acaaaatctg catcgatccc attttttact
    10981 aatacatcat aaaaatgagt cagttcactt aaccacaaac ctgtaggacg ctcgatcttt
    11041 tcgtactttt ctacatttgt taatacgatc aatactttca ttttcccatc ctctttctat
    11101 aaaaattgcg ttattgataa cgtgctttca agaaattata taatgggctt ggcaaataaa
    11161 gctctttata tgcttcttca aaaggtttcg ttacaaattc atcctcacgg ttttccaata
    11221 atttctctgc ccagtcagga tcgacgatca tttgctgtcc aattgctgca agttctgcat
    11281 atttcaatac ctgctctaca tcttcgcgat tgcggatccc accgacacca actaaaggga
    11341 tctttccttg aatcgtgtta tggaccactt caagaatcga tttatcgttg tatttatcag
    11401 aacgagctat tcgatcatac atatttaatg aaacatgcag ataatctagt tttgactcac
    11461 gtaatcgttc aagaagccag atcgtgtctt caaaacggat acctggtgtt tcatattctt
    11521 ctggtgagaa acgataccct acgatgaatg gttttttagc atattgttca accgctgaga
    11581 aaaccgttgt taataattct tcaataaaac gataacgatt ttccaagcta ccgccccatt
    11641 gatcctctcg tctattcgaa tgcggagaga aaaattgttg gatcaaatac gtatttgctc
    11701 catgcaactc gattccatca aatcctgatt caatcgctct acgtgtggct ttgccaaatg
    11761 cttggattgt ttcaacgatt tcttcttcac tcattgctcg tggtgtttcc gcatcaggac
    11821 gttttgctgc aactgcactt gcagatacag gctgttctcc agataaagtt gctcgacttg
    11881 tcattcgtcc accgtggaaa atctgaataa tcgcttttgc gccttctcct tggattgctt
    11941 tcgctagctc acttaatcga ggaatcattt catcatgagc gatgctgagt tcacctggcc
    12001 agccttttcc gatatcttgg acatttgcag caccagtaat aacagcacca agacctttag
    12061 aacgttgttt ataataagtg atctcatctt cggtgatcac tccattgaaa aagctgagtt
    12121 gtgtcgtcat aggcgacagc atcaatcggt tcttcaattg aactcctgaa ggtaaagtaa
    12181 ttgattgatc aaattgtgac attgtcattc ctcccataat tcgctctgat taacctatcg
    12241 agaatcatct atcattaaac cactcgatta gttgtacatg caactaattt gcttaaaaaa
    12301 ttttattttt tttgtaactt atctgttgca tcattcattg tctgggctaa ttctttactt
    12361 gcttcatttc ccaaaactgc tttgtatgac tgatgaagtt cttcccagct accatcgact
    12421 tccttagcaa attggcgtcc ttgttctgta agataaacta atgacaagcg accttcgcta
    12481 actgttttga cgtatccttt atattctaat ttttcaacaa aacgagcaat cgttgatggg
    12541 gcggaataga gcatttgtgc aatatctttt tgtggtattc catctttttc ttttaatatc
    12601 aataaaataa aaccataagt tggtgccaga cctgtccttc taaacgcttc atcagctaat
    12661 ttattcaaca tgcgatctag ctttttcacg ctaaagaaaa gacattcatc taacgctttt
    12721 tcaattggct tttccatttc atcccctcca ttggcgtaca tatcatagta tgatttttat
    12781 tttttgtcaa aaaacacgga ataatgtccg cctcctctgt ctagatttga aaaaaagaag
    12841 tagccatctt caaagaattt cggcttgttt tcgacaaaac tccttctgga acaccgtgaa
    12901 tcagaggttg tgaaaaagtc gtttagtttt gagtatgaag aaaaattttg tgaaaatggc
    12961 tttccacatt tttgcataaa tttggcttaa taccgaaaaa ctggcttttg aacaccgttt
    13021 atatcagagg ttgtgaaaaa gccgtcctgc atcaagtaat aagaacagcc aaacaaaaat
    13081 agttcctcat attttgtcgt tttttgagct aatgccgatg gtcagctgat tgtacaccgt
    13141 ttattgagag gtcgtgagcc tgccgttgaa ctccgagtca caaggagcaa tttcaaaaaa
    13201 cagcttctca tgttttatga ctatggtgac ttgtgatcga ggagttggca ggagaatact
    13261 gtttattcgt aaagaagggg ctgtgacaag acttttgtca cagtccctta tttcctttta
    13321 ttataggatt tcgccatttg attggatgac ttgtctgtac caatcaaatg attttttctt
    13381 taaccgtttc tttgtgccat tcccttcgtc atcttgatca acatagatat atccataacg
    13441 ttttgacatt tgtgaagtag aagcgctgac gatatcaatg cagccccacg acgtataacc
    13501 aaataaatct actccatctt tgatagcttc cttcatctgc tcaatatgct gtttcaaata
    13561 gtcaatgcgg taatcgtctt gaacggttcc atcttcttct actttgtcta tagctccgag
    13621 tccgttttca gctatgaaca gtggtttttg atacctatca tataattgat tcaaagcaat
    13681 tctcaaaccc ttaggatcaa tctgccagcc ccattcactt gatggaagat acggattttt
    13741 aattcctcca gttattaagt ttccttggac cccttcccaa ttttctggat gagcactgac
    13801 tgcactagac atgtagtagc taaaagaaac aaaatcaaca gggtaggctg ctaagatttc
    13861 ttcatcacct acttctattt ttaagttgac acctttttct tcaaacattc gttgcgtata
    13921 cgctggataa tatcctcttg cttgaacatc actaaagaac aaagaagttc tttgctgctc
    13981 aattgttttt tgaatatcat ccggatcaca agaatacgga acaagaagct gatagctgat
    14041 catacaaccg acttgtgaac ctttgataat ttcatgacaa gcttttgtag ctaaagcact
    14101 tgctataaac tgatggtgtg caccttggta tttcgcttgt tccaaatggg gatggttttc
    14161 ttcaattact cctacgctaa caaaagggtg atgttttaca cagttgatct cattgaaagt
    14221 catccaatat tttactttat ttttatagcg tttgaatact gtttcagcga aacgaacgta
    14281 aaactgaata agctttcgat taggccatcc accatattct gtaaccaaat ataacggcat
    14341 ttcataatgg gaaagagaaa cgactggctc aatcccatat tttagacatt cgtcaaacac
    14401 ttgatcataa aaaaataatc ccgcttcatt gggtgtttct tcatcaccat gaggaaaaat
    14461 tcttgtccat gcgatagaca tccgatagca tttaaaaccc atttcagcaa ataaacggat
    14521 gtcttcttta aagtgatgat agaaatcatt tccgtgtctt ttaggataat aatacttgct
    14581 gttgggatcc tgcgccgctt ttacttgctg atgcgtcatc atatgccatt gatctgtcca
    14641 tttttcttta ggtaaatcgc tgtcgaatgt atacacatca gacacagata atcctttttc
    14701 atctacatcc catgcacctt cacattgatt ggccgctgtc gctccgcccc ataaaaatcc
    14761 ttctggaaat tttttcatat cgatccccct aaacttgcta gttttcttgt ttgattattt
    14821 ttcttatgac tagtaatgat atttttgaat ctactactat ttgtaacaat aaccattgtg
    14881 gtcggatcat atccctcttt gattaattct tgcaaatcta cttccatcaa taaatcgcct
    14941 ttacgtactt tttttccatc agatactgca ctttgaaagt atttgccatt taaattcact
    15001 gtatcaatac cgatatggat cagtatctct gctccttctt cactgactac cccaattgca
    15061 tgtttcgttg gaaaaactgt tgaaacttcc ccgtttacag gactgtagat ttttccgtcc
    15121 gttggaatga tcgcaaaccc ttctcctaaa gatccactag caaatgttgg atcgtttact
    15181 tcagataaat caattatttc acctgctgca ggagtactta acactatatc ctttgaggat
    15241 tcttcttcga tttcatcctt gtagccgaaa agaaaagtta gcatacagga actgatcatc
    15301 gctaagaaat aactagcaac tagccaaaca aatccttcac caatataaat cgggaaagac
    15361 aaaatacttc ttgtagcaac tgccaaagca ccggctcctg cggcaccagc tactccacca
    15421 aatatagctc cacatataca agcaatgtaa aatggtcgtt tatatttaag agtcacacca
    15481 taaacagcag gttcagtgat tccaaataat gctgtgataa atgcagataa agataaactt
    15541 tttactttct tatttttcgt tttcaaaaat accccaagta ctgctcctgc ttgcgcagct
    15601 acggcagtca tacatgctgg acctagcgta tcacgaccgt agaaagaaag attgttggtc
    15661 attactggta caagtcccca atgtaaccca aaaatcacta acacttgcca aaatccgccg
    15721 ataacagcac cagataaaac agggctaaag ttataaacag ctaaatatcc agctgcaatc
    15781 agctgacttg cataatctgc aattggacct acaactaata aagtaattgg gaccatcacg
    15841 atgatacaaa tgaatggtgt caataaattt cgaatacttt cgtgcaagct tttgcgaatc
    15901 ttaggctcaa tataagaaag aacccataca gctaaaattg ctggaataac cgtacttgta
    15961 taccttgcta gaatcacggg catccctaga aatcgcaagg ttatgctatc actatatgca
    16021 ctgacaatcg taggataaac gagagctgct gcaataacca tagcgatgaa acgatctgta
    16081 ttgaactttt ttgcagcagt atatgccaaa aatataggca agaaatagaa aaatgcatca
    16141 gctgctgcat tcaaaatgat ataagttcct gattcagtag ttaataaatt caaactcgta
    16201 cataaggcga gtactccttt gatcaaacca cttcctgcca agacgcctaa agtcggggca
    16261 aaaacagcgg cgatcatgtc tataaaagag tttaaaatgc cttttttctc agttcctctc
    16321 tttccactag atgcatcatc ttctagtttc atttgtttgg ataatacgtc atacacttca
    16381 tttactgcat ttcctactac gacttgaaac attcctccag cttcgactac agtgatcaca
    16441 ccttctagtt gctctacctt ctccttatct gccttcttat tatcttttag ggtaaagcgt
    16501 aaccttgttg cacaatggta aacattgctg acattttctt cgcctccgac aaatcggaca
    16561 atatctttcg ctaattgtga atagtccatt tttacttccc ccttgttaat taatgccgta
    16621 gcaatccttc ttacaagata agtatagttc ttagaaaacg gtttctccat tcaattagta
    16681 ccccttataa tagcgaacga aactgtacaa tagtgtgtat ggtttactta gccgagcaaa
    16741 tcactcactg cttgaaagaa aacaagtata tcgtttctct tatagtcaaa ggagacaaaa
    16801 aaaacacgct tgatcttcct ttgctgatgt ataagtggta tctcaagctg atactctgtg
    16861 atttttgttt ctacttggct tttgataaga cagcacaaca aagtctcggc atttaacgga
    16921 agcataacta ggtcagaact aggagagatt agttgctgaa tggaatctat ctttttatgg
    16981 atttttattt gattttcttc tttgtagtaa tgatcaagca gttttgtttt ctttttttct
    17041 tcaactatgg tcagcttttc acgcaaaatc tgataaatct cccaagattc aaactcatct
    17101 agaacactag gtagataaat caaatcatcc cactcaaaaa taagctcttg ctcagtagtc
    17161 aaaattgcat catattccgt tattttgttt gattgatttt tgaaaagata cactggttct
    17221 atccggcatt ctttgatcca catgttcata ctttgttgaa tctgtttttt tagtgtattc
    17281 aaaatactaa ttggctggtt actaacaatc agtatctccc cttgttttcg aaaaggattc
    17341 aacgcttcgg caagatacaa aacaaaataa gcgctttcag ctaaattggg acgtaattga
    17401 aaatgtattg ggaagaaacg ccgaattaaa taaatttcta aaggataacg ggagatgatc
    17461 ttttgtacaa aatgattcat ggtattatgt ccggcatcca tcctcgtttt catttgtttg
    17521 atatgggtta gcaaaaattc tgtttcatgg aacaataacg aaacaggaag atctaaaaaa
    17581 cgaataatcg tttgctcaaa tgctcttaga ttcttctcta tttctttgtt tttttcatca
    17641 ttaatcatca aataatttaa ctctaatagt cgattttcaa ttgcttgttt ttcttgattg
    17701 gaaaaagaat agggtagttc tgatgataac ttttttgtga gggtctctac catcggaata
    17761 aataatgtat acctgtttgt tttttctaaa agttcttctc ttcgaagaat ggtcaaaacg
    17821 agataccgag aaaatcgtat gaagtcctct cctgaaacaa tataattata tgatataaga
    17881 acctctttta atgtcttttg gacagtcggc atgatattgg aaaaaattgt actgatttgt
    17941 tgaggcaacc ttgcgtgttg gatcaccgtt tctgttccaa caagagataa gaaaattctt
    18001 tttgaatttt cttcagcaat gatttttagt ccttttgtcg acgaaacttc cagttctaat
    18061 ttaggattgc gctcgatcca acgcaaaatc gtttttattt gtaatgatac ggctgttttt
    18121 gaaagaaaga aaatgtctgc tatcttttgc atactgacat accctttttg aaacaataag
    18181 tacgtcacaa tagttgaagc tcggaaatcc atgctggaat agattttatg tttcgtcacc
    18241 tcttgtatga tttgctgttt agtttcagat tcaattgatt ctaaaaaata acctgaacgt
    18301 ttatttgact ggataatgat tttcttctcg tagatagcaa gcatattttt gatttcagtt
    18361 tggatcgttt tcttgttggt acctaaagct tcagctaatt cttcgcttgt tatccaatga
    18421 ttcttatttc ttaatactac aagaatttgg agttgtcgtt tatttaagtc catttgctta
    18481 cacctcctct ttgttataaa catacaggaa aaacctctca aatgacaata gtcacttgca
    18541 gaggtttaaa aatcatttga agaaagatct atattcaagg tttttaccat tagactggat
    18601 aatttgttta taatattcaa aactatcttt tttcaatctc ctaaagcttc cgctcccatc
    18661 attttgtcta tccacataaa taaagccata tcgcttcctc atttcccctg ttcctgccga
    18721 tacgatatcg attggtcccc accataagta accaatgatt tggcaaccat cttttattgc
    18781 ctcagcaatt tcctgaagat gtatttttag atactcacaa cgttttggat cgtggattcg
    18841 attcaattca tctggtgctt catctagtcc aatgccattt tcaactatga ataatggtaa
    18901 ttcataacgg tctgtcaatt cattcatgac ataacgaaac cctagaggat caattggcca
    18961 actccaaggt tcgggagatt tttccttcaa aaagggatta tcgaaaccat ttgctccgcc
    19021 agtatctacg cgcatttctg cttctttttt atagacagcg ctacgataat aactaaatgc
    19081 aatataatct gccgtgttag cttgcaagca ctgttcgtct ccatctaaca ttactggtgc
    19141 acagtcttct tccttccatg tcctgtaaat ataagctgga taatgccctc ggcacatgac
    19201 atcggagaaa aaccatgaat tgcgtttaaa ttgcagtgaa ccaaatacat tctctggatc
    19261 acagtcatat gcataagtcg ctaatgcaga aaagctacac ataataccca tcttagcatt
    19321 tggatcgatt ttctttaata attgagtagc acgggcgttc gctagaaaca tatgatgaca
    19381 ggcttgataa atcacttttt ttgttagtgt agcaatgggt tcaagtttat ctctagcagg
    19441 gaatgtaacc aatccagcag ctgcataagg catacgaaaa gcattattga tctcattaaa
    19501 ggtcatccaa tatttcactt tcccttggta gcgttcaaag acggtctgcg cataaaattc
    19561 aaaaaagtcg attaattttc gattttgcca ccccccatac tctcctacca atgctaaggg
    19621 ggtttcataa tgactaagag taattactgg ctcgatatca tatcgattta atgtattgat
    19681 tagtctgtca taaaatacga gaccagcctc atttggcttc tgttcgtctc ctcttggaaa
    19741 tattcgtgcc catgagatgc ttgttcgaaa agcttttaaa cctaactcct tcagtaaacg
    19801 aatatcctct tcaaatcgat gataaaaatc aatcgcttca tgagaaagat aatggagtga
    19861 ttcgtctaaa gcaatctccc aaatcttttt ttcttcattc cactgaatac taggatgctg
    19921 gttcatgatc ccgataagcg tatctgtcac attaggtaat ttgccgtctt ccaaccatgc
    19981 accttcacat tgattagcag ctactgctcc gccccataaa aaattttctg gaaatatttc
    20041 ctgatccata actatctctc ctcgctatca acaatttctt acttgaagta tagctcgatt
    20101 catcaaaaca agatattcta actatttcgg cagaagagaa gaaaaaaatt gaatgctact
    20161 cttcattttt aatatttata tataacaaat acataagacg atccccgaaa gaaaacactc
    20221 gttaaccatc taagcaaacg atttatttta cttccaacac aaatgcttca taaggtcgta
    20281 aatcaacttt gccgtctggt aactgtttca gaggataatt tgacacaata acccttaaag
    20341 cttcacgtgg aacagtgaac gtctgctttt cactggacat attacaaata acgagccatt
    20401 gctggtcttc atatatgcga gaataggcaa agacatgctc aggcgtcttt tccactaatt
    20461 catagtctcc ccagacgatg atgtcatgtt ttctacgcaa atcgatcaat ttcttgtaca
    20521 tataaaaaac agaatcttga tctgctaacg cttcttctgc attgatttct ttataatttg
    20581 gattgactcg aagccatgga attccagtag taaacccagc atgttcacgg gcgttccatt
    20641 gcataggcgt cctagcattg tctcttcctt tcgcattgat tgattccaag atttcctctt
    20701 ttgtaaatcc agaagaaatc ctttcgttat acatattgat tgtctcaata tcttttgctt
    20761 ccgcgatatc atcgattggg caattcgtca tgcctaactc ttctccttga tagatataag
    20821 gggtgccttt catcatatgg agcaagatag caaacatctt ggcactttct tttcgataca
    20881 catcgtcatt tccccaacga gaaacaattc gcggaagatc gtgattattc caaaataaac
    20941 tattccatcc ttcatcacct aaagatgttt gccatttgga taatactttt ttcagttcag
    21001 agatactcaa gggtttcaaa tcccatttgt ctttgccttc ttgctgatct aatcctatgt
    21061 gttcgaattg gaagaccatg gataattcat ttcttttagg attggaataa agtttagcga
    21121 tttcggtagt tgctccccat gtttcgccaa cagtcatcag atttttgtca ccaaaagttg
    21181 cttgattcat ttcctgaaga tactcatgca gtttaggtcc attacttgtg atttctttat
    21241 ccggttcttt gccaatcata tcgatcacgt ccatacgaaa gcccccaata cctttatcga
    21301 tccagaaatt catcatttga taaatctctt ggcgaacctg tttatttttc caattcaaat
    21361 caggttgacg cttgctatac aagtgcagaa aatattgccc cgttttttca tccaactgcc
    21421 aagcagaacc actgaatatt gagcgcaatc tattaggctc ttttccattg accggatcac
    21481 gccaaatata atagtcccga taaggatttt ctttattttt acgggcctca atgaaccacg
    21541 gatgttcatc agaagtatga ttcaccacca gatccatgat gatacggata tttcttttct
    21601 ttgcttcttc aatcagttct tccatttctt ccatcgtacc gaattctgcc atgattgctt
    21661 gataattact gatatcatac ccattgtcat cattcggaga ctcgtatact ggagaaagcc
    21721 aaattgcatc aatacctaaa tacgccagat aatccaacct ggaaataatc ccccgcaaat
    21781 ctccaatacc atcttgattt gaatcttgaa aacttcttgg atagatttga taaacaactg
    21841 aatggtgcca ccatttttgt tccatgattt tcctcctata aatggatgtt tctatttttt
    21901 gcttaatcat ttattttagt ctctctcact aaaaattgta acgctttcaa atagctattg
    21961 caccttgtta tcggtaacag aataaaacaa aaaaacagct gaggaaattc cctcaactgt
    22021 tttttgaaag gattgattta tgcagggaag aatgctgatt tcacaacttc tttataaacg
    22081 gtgttcactc agctgtccct tggtattagc tcaaaaaacg gcaaaatatg aggaactatt
    22141 ttttcgtttt tttctctaat actcaggcca gaacactgat ttcacaacct ctttataaac
    22201 ggtgttcact cagctgtccc tcggtattag ctcaaaaaac ggcaaaatat aagaaactat
    22261 tttttcgttt ttttctctaa tactcaggcc agaacgctga tttcacaacc tctttatttc
    22321 acattcaata tccacatgaa tcccacaaat acaaagaaca gaccatacat gattggagat
    22381 acttcttttc ctcttttagc tgctaacatg gtaattggat agaagataaa acctaatgca
    22441 atcccatctg aaatactata agtcaaaggc atgccgatca agatcaagaa tgaagggatc
    22501 gcaatctcta agtttcccca ttcgatttgt cgcaaagatt gtgccatcaa tactcctacc
    22561 ataatcaatg ctggtgcagt tacttgtgaa gtaacaactg atagtaatgg agaaaagaac
    22621 aagcctaaca agaagaaaat ccctgtagta atagctgtga tacctgaacg tcctccaacg
    22681 gcgataccag ctgatgattc tacaaatgca ccaacaggag aagtaccaaa taaggaaccc
    22741 gcaagcatag ctgttgaatc agaagctaaa gctttcccta cacgaggcat cttgttgtct
    22801 ttcatgaaac ctgcttggtt tgctaatcca actaaagttc ctgctgtatc aaagaaagtc
    22861 acaagcaaga atgtcagcac tacgacccac atttgaagag aattgatgtc ccctacatgt
    22921 tttaatgcaa ctaaaaatgt tggtttcaaa cttggtgctg cggcaacaat ttcagaaggc
    22981 attggaatca aacctgtgac cagacctaaa atcgttgtag ctgctatccc gatgaagatg
    23041 ccacctggta cacgccgaac aagcaagata gcagttacga ctaatccaaa aatcgtcaac
    23101 caagtagaac caacgttgag tgatcctaat cctactaatg tagattcatt cgaaacgatg
    23161 atcccacctt cgctcagacc aaggaaagcg ataaatagac cgataccgcc agaaatcgca
    23221 tatttcaaat ctgctgggat cgcatcaatg atcatttccc gcagcttgaa gattgtgatc
    23281 aaaataaaaa tcaaagaagc aacgaatacg ccggctaatg ctgtttccca tggaatcccc
    23341 attcctaaac aaacagaata agcaaaaaaa gcattgatcc caagagcagg tgccgttcca
    23401 attggataac gggctaatac ccccattaaa acacagccga tcgcactggc taatgcagtc
    23461 gcagtaaaca ctgctccttc atccattcca gaagcaccta atacagttgg atttacaaat
    23521 aagatataag ccattgagat aaatgttgta aatcctgcca gtacctcacg ttttaccgtg
    23581 gtgttcaatt tttctatttc gaagtaggaa agtatttttt ccttcatctt ccatccccct
    23641 aaatccgaac attataaata aagtccgtta aaatttcgat ttattaataa aacaacgtgt
    23701 tttatcatat actaaaacaa aacataagta aacaaaaaat tcggatttta agcaaaatcc
    23761 gaacgttcca tctgattaaa tccctttatc tcaaagttca tgaacattca ctttggatta
    23821 caagaatttc tttattgtgt taataaagtt tcttcattct agtctcaaaa atcaatagtg
    23881 tgatttgatt ttaccatttc gtttctgttc ttctttgatg aattcttttg tatcgacagg
    23941 attcagatta tttagtccgc caaaacctgt atcgaccatt tttccccagc cttttgtctt
    24001 cttttttcct gatttgagat tctttgctcg aagcttctca ctatgttttg ctcgccgata
    24061 cttcaaataa ccttctaatc ttcccattag atccactgtt tttcctcctt tttattcaat
    24121 cagacgactc agcttttcct ataacaaaat aattgccttc agggtcagaa aaattgaaag
    24181 tcaagatgcc attgttatct gtgatttctc ctggttcttc catgttctcg tagatagttt
    24241 caaattcatc cgtaaaaaat actaaagaag gcggtggtcc taatacctct ggtgaaaact
    24301 tctgcacaaa tacttttggg aatacgccta attctaattc ttttgagatg ctgagtacaa
    24361 tactcttaaa ttcttctggc aattcgattg tttcagaaat ttctgcacca attttttcta
    24421 caaagaatcg gcaaatcatc tctacgtcat tgacataaag catcacacgt gttttttgta
    24481 tcatcatttg acccttcttt ctatttgatt ttctttcctg tctataaact gattccatta
    24541 tatcacgaaa gcgcaaacac cttgtgtttg atactattca agaaaaataa agcaatagtt
    24601 tctaggattg ctttcctcca caaacttttt ttacaaaaaa aaaaaacggc aatgaatgcc
    24661 gctcttctta tttcgattct tttataattc tgcttctgat tcatctttgg ttagtgtgtg
    24721 tctaacacta gttacgccaa atgtttgatc gattttttcc ttcaacgctt cgattgcctg
    24781 tgcgccacgt gccgcttgtt cagcattgat ggattcaatg accagcaccg ctgcttgtgt
    24841 ttcttccgtc agcattgttc tttgggcttc acgccagttg agacagcgac agatcgctcc
    24901 ttgttcatca tagtagatga tttcttccgg aagtgccgga gcatcggaat ctgcacctaa
    24961 aggcaggaat gattcgccac cttgggcttt tcctaaatgc agatttcctg cgatcttttc
    25021 gatatcttct ccgccgcaag gtacgccaaa agacaaggaa atactattat agatatctac
    25081 aagcggattg attgggaaaa attcccgttc ttgatttgca cgtttcagta aagcttcaat
    25141 agaagaacgt gcaccttttt tagtcttgaa tgtggtaaat gcctgacgcc actgagcaat
    25201 gacttcgttt tggctgaatg tttcttctgt aaggtaattt cttgcttctt ttgctgcctc
    25261 attcaataat tgatgatacg tttcttcact ttcttttgtt acgtggttat cgataccatt
    25321 tacggataag atttcgatgg ttgcttcagg aaatacttcc cagaaattgg tatccactat
    25381 tacttttttc atctgttcac acctcttgat tagaataatt gcttgatgaa tagatagtct
    25441 gtttggggat catctcccat ataaaaaaca tgttcgctgt atttgataaa tcctttttta
    25501 cgataaaaag cgagcgcaac atcgttgtgt tcccaaacac cgagccaaat ggctgttttt
    25561 tgcttttctt ttgctttttc aatagctgta tctagtaaaa atgtaccgat accttgatgt
    25621 ttaaattttt ttcgtacata gattcgctcg atttctaaaa aatgatcagc catttcctca
    25681 gattgactcg ttcctgtatt tagtttcagg taacccgcag gctcttgatc gatactcact
    25741 aggaaaaaat cggtttcttt atcttttagc tcttctgtaa gtttttctag tgcataagct
    25801 tgtgtcaagt aatcagctaa atgctccggt gaattatctt ttccaaatgt atctgtaaat
    25861 gtttcgatac taatttgttg tagtaagtct acatctgaag gcgtcatttt tcgaatttcg
    25921 tatttattca cggcttcttc ctttcaatag atccttttat tgcctttttt gacaaaattc
    25981 caatcttcgc taacattctt gcatacttta tttaaaagat attccaattg ttttgcttct
    26041 tcgtcagaaa gaccatttaa tgcgactgca ttggagtagt tgttttctcg aatgatgaaa
    26101 ggatagattt ctgctccttt tttagtcgga tagagtttct tgattttttt attttcttta
    26161 tcttccaaac gttcgatcat cccattggat tctaatttct tgattgctcg ggcagtcgtt
    26221 gtacgatcta ctttgatcat ttctgctagt ttttcttgga tgattccagg attttcgcaa
    26281 atacgtacta aataaagata ctgtcctctt gttaaatcga cttctttgaa ttcgatattg
    26341 ctaatagaat ctagcgctcg ggcaatcatt ccgatgtctc gaaggatttc agccatttca
    26401 tcccaccttt attttgtttg taagactagt ttacctcttt ttgttgcaaa tgcaactatt
    26461 ttcaataaaa aaatcaagtt cttttttaat aactagtaaa gtatattctt ttcttctttt
    26521 acgaaaaagc tcaatcacaa tagatctggt ttttgttttc taaaataagt aactgtttct
    26581 tcgttgttcg cttttctgat agctttggca actcttctat actaaaaaat cccatgttgg
    26641 aagtttcatt attctcaata aacttggcct cttcaccgtg gatcgcacaa gcaaaaacaa
    26701 gtttataata ctgaaaaagc tgtggaatat ctttttgttt attcgtatca aaaactgctc
    26761 gcaactgagt agtttctact gttaatcctg tttcttcata gacttcttta cgcacatttt
    26821 cttcaggaga caatcctatt tcagcaaatc caccaggaag tgaccattct ttcgtccgtt
    26881 tatcttcgat tagtaggacc ttgccatctt ttttgatata ggcacgaaca tctacttttg
    26941 gtgttggatg tccttctcca atcgttgtga gcgcttttat ttcttcgaaa gtttcttttc
    27001 ctatttcact gatcagttcc aatgaaatcg tccggatctc ctcaaaacgc tctttatcaa
    27061 atacatcttt tccataatat agaccagctt gagaaagtgc gagtagtctt ttgtattgag
    27121 ccaacttctt gttcattctc ttcccttctt ttttagcaaa tgtatgacta tgtaatcaag
    27181 tatagaagac tggtttaatt acggcaaata aatataatga aatctgcaat aaataaaaga
    27241 atgttaaaaa actcgagaac aaaagacatt cgacttctct ggggctgtga ataaatggcg
    27301 ttcaaaagct tgcatctgcg gtaataagcc ccaccaaacg aaaaatatga cgagctattc
    27361 ttgtctggct gttcttatta attgaggcag gacagctttt tcacaacctc cccactaaac
    27421 ggtgttctcc taccaactcc tcggtcacaa gtcacaatat tcatcaaaca tgagaagctg
    27481 tttttttgaa attgctcctt gtgactcgga gctgaacgac aggctcacga cctctcggta
    27541 aacagcgttc aaaagccaat ttttcgatat taagccattt ctttgttact tattgtcatt
    27601 gatttctttt tctaatttat ccaatagtgg agtgtaatcc acttgagtat agtctgttac
    27661 atcaaattca tatagtgttc ctaactggaa tttgtcatat tttcggtcgg cgatgatctt
    27721 tttgaatgct tctttcgtaa tatgattggt ccctttcgtt cgatcttcca atacgtcacc
    27781 atagtgataa tgatccatga tatagctcaa atgacgatct gattcacgat cacgtttgta
    27841 ccgacgttga cataacactt caaagtctgc tgttaggcga atcgtgatca cctgatatcc
    27901 gtatctatct gctaattctt ggagtcgtgc tttttgctta tcactaaaag gatactcgga
    27961 cacaatcact ttctttcctg cttccatata caaatcaagt gctttgtaat agaaaggata
    28021 aactttcgtc ctttcttgtt ccgcccgttc ttccaaattg ttaaacccga tggattctgc
    28081 atagtattcc ttgatttcat cgagcgtgat ggcatacata tctggaaatc gttcccgtag
    28141 tttattcata agatatgttt ttcctgtacc aggactacca gccagaagaa tgaaatactt
    28201 tttcatctca tttatttcct aagttaattg gactttccaa gttcataaac agtccaatct
    28261 tatgcttgac aatttctttt actgcttcat ttgctggaat cacattatgg cgaagagact
    28321 tgttgatttc tgtttcacta aaggtttctt tagctacttt cgtccagttg actaaaagct
    28381 ccgttccaac attgaatttg cggatcccgt tattgatcaa ttcaggataa tcttcttctt
    28441 tcacacctgt tcctccatgg ataactaggg ggacatcaac tactgcatcg atttctttca
    28501 gcaagggaat gtttacttct gtttttgatt tgaattgtcc atgattcgta ccgatagcaa
    28561 tagctaatgc atccacgcct gtagcttcta caaatttcac tgcgtcttcc ggtcttgtat
    28621 ataccttgtc attttcagcg acatggattc cttcttctgt accgccgatt gttccaagtt
    28681 ctccttctac ggaaacaccg tgcgcatggg catattctac tactgctttt gtcttcagga
    28741 tattttcttt gaaaggcaga tgggacccat catacatgac agaagtatat ccgctgtcga
    28801 ttgcttcttt gatatcatca aagtctcggg cgtggtctaa gtgcaagact gcatcaacca
    28861 tttcttcttc tgcaatagct gtcattacat tgactaacac atgcatgccg atatatttag
    28921 cggtatcgac acttgtttga ataatgatcg gcgaacccat ttcttttgct gcgcctagca
    28981 tagcaggaag catttctagg ttatgcgtat taaaagctcc aactgtaaaa ttcaactctt
    29041 ctgctgtttt tgttacttct ttcaatgttg tatacatctt ttattgccac cttatctttt
    29101 tatttgtttc ctttgatcgt tacttgtttt gcaatttgtc gcatctcatc cattttgccc
    29161 atgtagtaat cgatcccttg atcatcacca gcacgagcag catctgcacg cttctggtta
    29221 tagaggctca tcagtttttt atacccatct ccgatcgtct gtttattttc caaactcttt
    29281 tctaaatatt ggatggcttg atcgttcaca tacaattcag aaaatttaag tccaacttgt
    29341 tcataaagtt tcgctaattt atgcttatct tgggtagtgg aaatctcttg tttcaattgt
    29401 gtaatctctg attgaatctt ttcggtcgct tctggcgata gggctttttc ttcttttgcc
    29461 agaacttttt tcggttcttt ttcttttttc ttaaaaaatc caaacatcat tttccctact
    29521 ttcaattaaa atgctttcaa aactgggctt aagatccagg catataagag tgctgcggct
    29581 actgtatcta catcggttgc tgtagcattg atgaatccca tcgaattaaa gatcgttact
    29641 agcaaagcgg gcaataatgt aataaagaaa ccatgagcaa taccgccaat gatcgctcct
    29701 cggcgtccgc caactgcgtt gccaaatatc cctgctgtcc ctccggcaaa gaaattggtt
    29761 agcataccag gtaaaatcat cgctaagccg aacatgggca aagtaaacat ggcaataatc
    29821 gtaccaatcg ttgtcgtaat gaaacctaaa atgacagcat ttgggctata aggaaagaaa
    29881 actggacaat ctaaggcagg gatcgcatca ggaactaatt tcatagctat ccctcgaaat
    29941 gccggcacga tttcacctaa cagtagacgt acaccagcta gcaatacata cacacccact
    30001 acgaattgga tggcttgtaa aaaggcaaac atgatatagt tttgatctcc tgataattca
    30061 gaagcgaatg gttcgcctgc aaataaaaca gtgatgatat atagcgggac catcacgacc
    30121 atgacagaca aataagtatc ttgcagaaat tcgaaatgtg tcggcaactt gatatcttcg
    30181 atcgactttt tattttcgcc tttctcacca aaaagcttag caacaccggc ttcaaataag
    30241 tacccgatcg tacaaaaatg acctaaagca atatcatttg atccagtgat tttacgaatg
    30301 atcggttgcg ctactgccgg catagcaatg gcgaaacaag ctccgataaa accaccgacc
    30361 aagatcaata cgatccctcg cagaccggca aaatagccaa agactgtcgt catcgttgcc
    30421 atccataaaa ttgcttgacc agttaaaaaa atgtacttcc attttgtgaa acgagccaaa
    30481 ataatattga atacaaaaat ggctaagaac gtcagtgcaa tatctctacc taaacccaaa
    30541 tcattcattg cttgaccatt gatagattca atagaaggga tgatcccttg catatgaaat
    30601 ccttcagtaa agattttccc gaaataagtt aaacttccaa cgatgatgct cgaaccggca
    30661 ctcaacactt gaaatcctag gagtgtcttt aacgtgccag agatgacttg tcctgctgat
    30721 ttcttctgca gtagtagacc caacatagca atcaatgcaa tggtgatcga tgcctgagtc
    30781 aaaatatttt cgatgataaa attgataacg cccatgtttt taccctcttt tctttttgta
    30841 tttagataac tcccttatct tgcagaactg gggtgatttt ttcttcaatt tcattttttg
    30901 aaacaatatt tttcaaatag atcatcgccg ttttaggatc aactgcgaac ttttcgaact
    30961 gcgaacgaaa attttctgct gtgatgatca catctggttt catggaaact gcggaagaaa
    31021 tatcacaatg gtccagtttg gcttctactc ctaatttgtt caaaacatcc tcagcagaca
    31081 tctgtgctgc aaaactactt cctaaacctg caccgcatac gaataaaatg ttcaatttgt
    31141 tcatctttgt tctcccccgt atcatttatt atttctcaac tactgaaaaa agcagttgtt
    31201 tgaaatcttc gatatttgtg cattcaatca atcgatccaa cttctcttcg ttgttgatca
    31261 attcaattag ttctttcatg atgttcagat gcgagtagga gtctactgct gccaaacaaa
    31321 aaataatctt tactggatcc atttcaggat taccgaaatc gattggttgc tcaatcgtag
    31381 ctacgctgac tcctaattta ttgaccccat cttctggtcg ggcatgggct agtgccatgt
    31441 gtttgccaat cacgatatac ggtccgtaat cattcactgc acgaatcatc gcttcgacat
    31501 agcgctcttc gacaatgttt tctttaacca atggtctagc agctagttca atcgattcct
    31561 cccaagtagt ggctttttct ttgatcatga tattgctgtc cgtcaaaata tcttttaaca
    31621 taggctggat ctcccttttg ttgatttcta aattgtttgt ttcgaataat ttttctaact
    31681 tgccatagat gattcgatct actcggccac cactttcttt catgacatct attatttgag
    31741 tgaataactc cgtcgattca tgactgttag gctctaaccg ctgtagttct tcatttcttt
    31801 tcaaaaagtc ttctataacc aagatattcg tctcagttaa aatcggatcg acaactaaaa
    31861 gcggcttatt tcgataattc aattgaaagg tgctgaaaac cagattgaca tccaatttat
    31921 ctactaattc gatttctttt gaacttaaaa ctgcagtaat ctcgatctga ggaaactttt
    31981 ctttcagatt ctctgctaac agatttccag tagccatccc atggttacag acaacgactg
    32041 atttataaac ataattcaag tctcgcttga ttacactggc gatcgtcgaa aagtggatga
    32101 caagaaaagc tatctcgtcc tccagtatct tgccaccaat tacttcttcg atcgtaggaa
    32161 caaaatgagt gactgcgcgg tagattggga aataatttgt tcggatgttt tctttcaatg
    32221 gattcatgac ttggatatga ttcttgattc ttacaatcag tgcagccatg tgcttataga
    32281 gattttcgaa taaagcttct tcctttagat ggaaaggtac gtgtgtctcg ttctctacaa
    32341 attggatcaa ttggatcgtt agtaattgag cttgtaccca ctcgattgaa ttggagatat
    32401 ctctagcatt gaacgtctca atagtgaaac gaagataatt tctttctact tgcgggacat
    32461 tgtccaattg aaattcctcg atcatgtggt caataaatgc agaaaagcta ctgtctttta
    32521 tttctgtatt tttccaatta atggcagaga taaattcatg tttcctgatt cgctggatcc
    32581 agatcaatgt aaaaagcagg atttgttttt tataaatatc atcttcttgt ttcaacaact
    32641 tattggaata gatctcctgt atttttttga tttccaccaa aggaaaataa tggagaaaga
    32701 ttttttgagc cggtgttaat tgtgcatctt gattcataaa gaagtcggtc attcccagat
    32761 tctgattgat aaaatcatag atcatcgtac ggatagatcg ttctgaacct ttataaacca
    32821 gtccttgctt tccataactg acgatctcta tcccataact gaccagtgtt gctcgtaacc
    32881 gtctcatatc ttcatctaat gtacttttag agatttgaaa ttcttcttct tttttgttta
    32941 gatggatcgg gattcggctg taagcaacgg ctaaaactag atcaaaaata cgttcgcgcc
    33001 gattcaaaaa gccgtctttc acatctttta tttcagtttc taatcgagct tgcttttcct
    33061 tttccagctt caacaaaaaa actttgttgc gaattgtacg gatctccgga agctttttac
    33121 tgctcaacca gatattcgct tcaagaactt cctgacggat cgtccgctga ctgacattga
    33181 actcacttgc tagatgtttt gcttttactg gatattgggc tgtcatgatt ctgtgaagta
    33241 tttcaattgt tcttgttttc atagaaatga tctccttctg atttcaagta tatggttccg
    33301 cttacataat aaaaagtaga tatttctgcc acttgaagtg gaaagaaagt aagcaaagca
    33361 aacaaaaaat aagccatatg ctttttcatc tcctcaacga aaaagacata cgcctattat
    33421 tacaaaaatt ttgcccatct atacaaaaaa gaggttgtga aaaagctgtc ctgcatcaag
    33481 taataagaac aactaaacta aaatagtgct tcctattttt cgtttggttg ttcttattat
    33541 cgcagatgca agcttttaaa caccatttag tggagaggtt gtgacagaga tgttcagcct
    33601 tgagcgataa gaagcgattt tgaaaaatcg cttcccatat tttacaaaat cgtgccttat
    33661 tgccgatagg ctatctctgg cacactgttt attcagaaaa aggagctgcg acaaggcttt
    33721 tgtcacaact ccgttcataa atatttcctg tcaatccagc ataatcggct gaataaaatc
    33781 ggcttgccat cttttttctt tctcagaaaa atcaaaatac catatctgag aaaaatactg
    33841 tcgttgtgtc ttacttcctg agatcaatcg cccatctttt cgatcgactg aataatcaca
    33901 gcatctgaaa tcaattcgga tggcaaaact attttcgtgg atctgtcgat aatttcctac
    33961 ttcctgaata gttatttttt tagtatgatt tcttacaccg tctgctttgt ttttttgcag
    34021 aactttctga tgttcggcaa acagcttgtc tgtataacag cttctggctt tttcaggagt
    34081 ttcttcttcc caagcggatt ggatcttcat gaatatcttt tggatttcca gaaactgtgc
    34141 tttcttttct ttagccgttc ctgacatgcg ggacaatatc tccgctttca gccttttctt
    34201 ttgtctttta ttcttgacga tagaaaaagc agaaacccct cctactgaca aaacagctaa
    34261 tgcaagaaat cccgtttgct cataactaga aggacctgta cctggaccat aatacccata
    34321 agaatctctg ccgtaagaac gactgccaaa ataactgccc ccatatgaac gaccaccaaa
    34381 tgaacctctg cccgaacttc gaccgctaga tgtacctcca ccatgaccac ccgcgacagc
    34441 gaatccttgg gtcggcagag caaacaaaca taggcccact aacaaaatga ttgctaaata
    34501 attgattttt ttcattgtct tcctcgttat cttttattac aaaaaactat ttttatgata
    34561 gccttgctaa aaggaagagt caataaccag atgcataaac tgttttttta gcaattttgt
    34621 cttatttagt cattgagtaa catcggacaa atgtatgtgt cggcagtcca taattcctct
    34681 atcttcagca tgaacaagct tgcagacaag agatctgcgc ttcctccagg agagatttgg
    34741 ttttcctgac aaaagtgcaa cagtgctgta aatggacgat ttggtaaagc actttgacta
    34801 gcagcatgag caagtttttg catttccact aacgttttct gatcactgcg atgcaatacg
    34861 catgtgtcat ctagcgaact catcaaaact aacaacatat gcatttgttt ctgtacatat
    34921 gaatcattat ctagctcctt agctgcttgg attgcaagcc tgatatgcgg ataccctaat
    34981 atcgcttctt catatgcacc atttacgcca tatttttgct tcaccctatg accatgagtg
    35041 gtttttttat gacgatattt tgtatcagga aaagcagcaa gcttgctgac atcatcaagt
    35101 attttcgata aagaaaaagg ctgttccata ctgatttttt gtgcaatgac actgatcaat
    35161 agccccattg cccaaatcgc tcctttatgg gtattgatgc catttgtagc aatcagcatt
    35221 gccttttccg cttttcgccc aatctcagca attctttctc ttaactcctg atcaatagca
    35281 tgatgccatg ctatttgagc catttcttca aaaaaaggag ttagcgtctt agcagactta
    35341 agaaataaag catagtccat atcggaatgt gcacccgcat ccagtgcatc cactaatcca
    35401 ggttttggac ttaatgttac ttctgcgatc aatgcttcct ctaccaacgc agcgagttgc
    35461 tgagtcactg ctttttttcg ttcgtcattt atcatgacac tcactccttc ttcctactat
    35521 tacttgctga aacctttttt gatagctacc caaatgatat gaagtaacca gcttaaagca
    35581 aatgcacaac ttgccgtcat tagataagct acaagaatca tgactcctga aggtaactgc
    35641 agccaactat ctttaaacaa taataacagt acttggaaaa caaatacatt ggctaaatac
    35701 gtacgataag cataaacaga caaccatttg aacaatggca attttggcga atcgtttaca
    35761 atcattttgg aagcgcctat gaagacagca aagatgatga ctaaactgta taaactttgg
    35821 atcgtattca agtaaggtgc attcgcaaag ctcaagtctc ctggatgtgc gagcaatgaa
    35881 cggacagaaa agaacgcgat aactaaggca acaggtaaga aaagataacg tactctgtcc
    35941 aaatattgat agactttttc gtgataaaca aatgcggcta ccccataaat catgaaacta
    36001 gcaacaaacc gatctaataa gtaccagctg tcatgataag gcccagtcaa tacttgtgtt
    36061 tgatagaaca gataccatgc gacatataaa atcgttgaac caatcaatac tggccataca
    36121 tattttttat tagcaaatac tttgtaaccg agccaaacaa aaaacggcat gaacaattgg
    36181 atctgcagca tcataacggt ataccataaa tgaggcgctc catcacctgt gacatatttc
    36241 agtaaaaagc ttgcagtatc cgtatacgga gtttgttgtt ggactgctgg aaatagccaa
    36301 agataagccg tcgtccatag aagatacgga acgactagtt caaaaaactt ttctttcaaa
    36361 aattcccaat aagacaaatg ctgacttgtt tttaccatat tataaacaat tgcaaaaata
    36421 aacattggtg ctgtgtattt aacgatgacg taaaaggtac caagaaaagc tgtatttgtt
    36481 gcgctgtggc tgtttgccaa agcaaagctc aatatcgttt gcatcataac agccgttaca
    36541 gctgatactt tctggaagtc acttacgttg attttgattt ttttgttttc aaacatctct
    36601 ttctctctct ttttctttat taccaaagtt ctttggttaa tttaggtcct tcgactgttt
    36661 tcaatagaat ttttttctct ggctctctca aatactcttt tagtgaaaaa ccgccttgcc
    36721 gagtcactac ttgggtatcg aattgctcaa aaaaagcagg atgcgattga attacctgaa
    36781 ttggcaattc aatcggtgaa tttgcgtaca gcagcaaatc aaaatcactg gtttctttta
    36841 ctgccggaat cccagtcgat aattcaaatc ctaagctgcc gccgattccc catttacatc
    36901 ctgataatat gtttctagca gcatgaaatt gctgataaac aggataatcc atgattttct
    36961 cttgtctata cgattcttgt tcgatgatgt cccacggatg aatgacttgt ttgacggcat
    37021 gttttgagat ttctgttgac catctctgat tgcgggcagg tccacgtaaa ccaatcggta
    37081 gtatttctgc gtgatccgtc gacgcacgac gaactgttcc gaacatcttt tcagttagaa
    37141 cgacccattc aggcaaggtg aacccttcta tttcatgttt atcaaaaagg acaatatcat
    37201 gtgcttgaat cattttttat gcatcccatt cttcgtccat tttggcacgg actttatttg
    37261 tttcgacacg tccgaatgtt ttggctgttt cgtttgtgta tcgatttgtc agcatcgtgt
    37321 catttgtatt gcggacatct tgaatggctt cattgattgc ttcgatgact ttgctgacat
    37381 tttgttctga agcagcttgg tcggtgatat cttctaggaa atgatagatc gctcctagtt
    37441 ttgtataatt attgatatcg taagcgatag ctggaacttt ttcagcagct gcttcgattt
    37501 cagcaaccga acgttttgtc acacgtgcag cactttcttt agacatcgct tggacggtaa
    37561 tttctttatt gtttaaagca atcaagcggc ttgattgtaa tccatgtgct aaaaatgcac
    37621 ctgaaatagc atttcctgca atcaatccaa taactggatg tccagcttga cggagttttg
    37681 cataagcagc agcactatta gccaaagcca catggatacc gatcaattct tcgttatagc
    37741 cgtatgcttg gcttggtaca tcgatcacaa cgatgattgg ccgtttcact tttttgtctt
    37801 tatcttcttc gtaaacgtga tccaaaattt gtcccatgcg gaaaccttca actaaaccaa
    37861 cttcaccgtt tcgtacacgg tacatcgggt ttttaggatc aggtacgatt gatgtcacga
    37921 tggcttcttc tatgaattta cttgatttac cataatacac agtactaata gaagaagttg
    37981 gatttttcat gccagttagt gccgcaaacc aatcgtaacc aacacttgct gcagctggtt
    38041 catttccttc tgctacttct ggtaagtcaa ttgattttgc ttgatggtta gcaattgctt
    38101 cattgtaatc gtcaatggcc attggttttg aaagatctaa agaatcaagt aatgataaat
    38161 aaaatgctga gctttcactg cgatgcgtgt cttttcgttc gtccattgct gcgatgacag
    38221 cagctttgat tgcttctaca ctatcagtca ctaattcatc aatgattttt gattctaccc
    38281 gttgacgaga tcctaatgta ttccaaatca agtctttgtc actagaatca aattctcgaa
    38341 cgcctgcttc ttgttcaata acttctggtc cattcaaacc gactctcgct ttttttgttg
    38401 cgattaaata actcattaat gctgaagtaa tcgacatacc gccaaatgat cctacacgtc
    38461 ctggaatcaa accgatgact ggtacatatt gttttagagc aatgattaca tttccgattt
    38521 ctgaaataga taacagtccg tagtttgctt cttgcaaacg aacaccacct gtatctagta
    38581 caatgactgg aaagatctca tttccttttt tattttcttc taacacatgt tcaagtgcag
    38641 caacgatttt tgcaccggaa acttcaccga taccgccacc ttggaatttt ccttccatag
    38701 cgattactac tgcttgtttg ccattgatcg tgccacggct gacaacgatc ccgtcatcac
    38761 tttcagggac gattccttgt gctggtaaac ttggcgcgat gatttgatcg aatggatcaa
    38821 tcaattcacg tccattgtca agtagagcgt tcactcgttc tctggcgttt aattctacaa
    38881 aactattttt catctgaaag ttcctccatt gcttgcgtca atcgtaggta cacaactcct
    38941 ggtgtagcac cgaagtcatt gatcacgata tctgctgaaa ttggataacg atcaaaaaat
    39001 ctcgttaata cattgttcca tacttcgccg aagccatcgc ttcctgtaca aatttttaca
    39061 gtagattctg ttttttctgt tggaaacatc aagatttcca aatctcctga accgaccact
    39121 cctacatgga ttttcttatt gatcggctgt ttcgttggat atttataagc taattgttcc
    39181 atggatctct cccctttttg taaaacagag catacacgtt ctgcttattc gtctttcttc
    39241 ttccatctgg gtaaatggcg acaaaaggtt tttctcgcca attttatgac caagttctga
    39301 atttcattgg tggttcatac aggccatctg accattcgat caaatcatcg atggtttgtg
    39361 cagctaataa tgaacgtttc gcttcactac gtttgatgcc tagatcttct ggtaatttca
    39421 cgatgccggc tgcacgcaag ctatctagtg attctttcgt gctagtcatt ccaagaggtg
    39481 taacaccgcc aattgctgca attgctgctt gtcgttcttc ttttgttttt gctttgtaca
    39541 aataagcaat tccttcttct gtcacgatat gtgtagtatc ttcgctataa atcatcactg
    39601 gtgcattggc taagcctgct tgtttttgaa cagctagtgc atctaattct tcgacaaaga
    39661 ctggtttttt atttgaacca tatgtttcaa ccatttgaac aactaatttt tggcctttcc
    39721 ctaatggatc atcattgtcg cgaagtgatt gccatgctgg tgtagaatga cgacgaccgc
    39781 caggattatg tcccatgtta ggcgcaccgc caaaaccaga taaacgtcct tttgttacgg
    39841 tagaagagtt tccttcgtaa tctaattgca aggttgaacc gataaacata tcgatggcat
    39901 attgtcctgc agcttgtgat aatgttcggt tactgcgcat cgtgccgtct ttaccgacaa
    39961 aaaagacatc tgggcgagca gcaatatatt tttccatacc aacttcgcca ccaaaactat
    40021 ggatgctttc aacccaacct gattcgatgg ctggaatcat cgttggatgt gggttcaata
    40081 cccagttctt cgcgattttc ccttttaacc ctagtgattc accataagtt ggcagtaaca
    40141 attcgatagc tgctgtattg aatccaatac cgtgattcaa agattgtacg ccgtgttctg
    40201 cgtagattcc tttgattgcc atcattgcca ttagaatttg caattctgtg atgttttgag
    40261 gatctcgtgt gaataaagct tctaattgat aaggtttatc tgctttcacg acaacgtcta
    40321 cccagtcacc agggatatct acacgtggta aagaatcgac aacttcgttg acttgaacga
    40381 tcacgatacc atctttgaaa gctgcagctt cgatgattgt tggtgtttct tccgtattga
    40441 aacctgtgta caagttgccg tctttatctg ctttatctgc agctactaaa acaacatttg
    40501 gaatcaaatc gatgaataag cgtccataaa gttccaaata agtatggata tcacctaatt
    40561 tcattgtgcc gtcttcaatc atttgggcga cacgcaagct ttgtgcacca gcataagaga
    40621 aatcgatctt gcttgcgatt cctttttcaa aaatattcaa atgttctggt cgtgagatac
    40681 tagacatgat caaatgcaga tcattcactt tttctgcatc tactttttca agactttctg
    40741 aaagaaaact tgcttgtttt tgattgtcgc cttctaatac aactttgtcg ccgggagcaa
    40801 tcagtttttc cattacttct acgatttttt cagtttctac atacttgcca ttgagcaagt
    40861 tgctgatttt ttccaatcgc tgcgcttttt tttcgcgttt agaagcccag ttgcggtttg
    40921 gtttttccat aattagtcat ctccattttt gatatagaaa cgtggcgtca tcaataccat
    40981 gttggtctag attgatcgta cggatccctt tatctacacc aaattttgta tgaatcagct
    41041 ttgtcaaagt attgccgggg ggaaattcaa tcgtcagatc cattcctaat tcttttgcca
    41101 agtccatcat ttggttccac tgcacaggga aagctaggtt ttccaacaaa tctttacgaa
    41161 tctcttctgc gtcctgagtg gctcttccat ccgtattttt tatatacaaa catctagcat
    41221 cagatagatg gacagactgc atatacggct tgaatttctt gaccgtatca ttcatcaaga
    41281 gacagtgaga tggcacaggg actttcagca gcttcgctgt ttgcgcttgg tgccgtttgg
    41341 cttctgccaa cgtaacggat aaaccttcga tactcccaga taacgtatgt tgcatcggac
    41401 aattttcatt tgacagataa actggatggt caggatcaaa tgtttttgct acaatttcct
    41461 cagcttcgct tctcgtcaca cctactacta cgcccattgc ataaccgctt ggataagctt
    41521 ctttcattaa gactgcgcgg tgatagacta gttccgctgc attctcaaat ctcaaagatc
    41581 gacaagcaac agctgcacaa aaagcaccaa tcgaatgtcc tgctgcaata tcaggctgaa
    41641 taccgttttt ttccatttca tctaaataaa atactgcttt tgtcagtaat gctagctgga
    41701 taaaaaccgt atcttgatag ttttcttctt tatcaactaa ccgatacccc gtcacatttt
    41761 ctaaacgttc catgtacatt tcagggatat ccgctaacat ccctaacttc tgactgcctt
    41821 gtccaggaaa taagaaacct gtactcatga gcctcgcctc ctcagcactt actttttcca
    41881 ctgctatctt agtcaatttc tgctataata aaaaatgcca aaaaaagaaa aaaccataac
    41941 aaaaaggtat ggatgggatg gataattatg aaattgcttc atctgcagta ttttaaaaaa
    42001 gtattcgaaa caaaaaatgt tacccaagca gccaaagaat tatttatttc tcaacctgcc
    42061 ttgagccgtg cgatcaaaca tttggaaaac gaattaggtg tccctttgtt ttaccatagc
    42121 ggcagaaaca ttgagacgac cgtttatgca gaagaatttt atccatatgc agtcaagaca
    42181 ttagacacat tagaagaagg aatcaaatta ttgtcttcgg tcaatgagaa gatcgttact
    42241 tccgtcatat tgtatttaga ggtagcctct gtttctattc cgaacttggt ccgaattttt
    42301 atggaaaagc atcctgatat ccaattaacg atcatgcaac atcatttacc agatgatatt
    42361 accgatcctg tactgtacat cacttcggag atgaagcctg gcatgaccaa tattcctatc
    42421 atgaaagaac ctgtttatgt cgccattcct aagaaacatc ctcttgctaa aaaagaacat
    42481 ctgcaattag aagatatcca acgcaccccc atcttgatgc tgtcaaagag caacgccttc
    42541 cgtcatacac tggatgcagc acttgaagcg aaaagtatcg aattgacgat cagttcgacg
    42601 acagatgatc ctgcgacatt acgttctatt gtcaagcaag gattagctgt tagctttttc
    42661 ccaaaagttt cttggtctta cgataaaaat gatcctttcg tcttgcggga aatcaaagat
    42721 ttgcccttga cacgaacaat ttatttatct tctagcttca cagaagatca tcctttgatc
    42781 aaaatgattg caaaaacgat gcgggaattt tatttaggaa aataaaaaaa aaagagctgg
    42841 gacaaaattc ttgtcgcagc tcttttttcg aacaaacggt gttccagaag taatttctta
    42901 gaaaataagc cgaaatttcc caaaaatttg agaagcaatt ttcgtaaatt ccttcttatt
    42961 tcttgaagct aaacatttct gtcacaacct cttctctatt gtcctagaca ttcattgcgt
    43021 tgatttaaaa cttccatcaa tcaaatctgc tagttcatct agcttttcta ttttacgtag
    43081 atcaatagag atccaatgtt ctttattcat gtgataggct ggaaaatacc cctcttttcc
    43141 tcgcaaaatt ccgccaagtt caggatcgac cttcaaatca agaatattga tgatcctatg
    43201 gtcacttgca tcatcataga ttttttcttc tggaacagac atcaggaccc caaaccattt
    43261 tttgcttttt tgatgtctga atacgcaata atcaggaaat tttttaaaaa tatattctgg
    43321 agacaaattg tatgtttcat tgatgtgatc gattagttct tttttcgtta gcatctgctt
    43381 ccatctcttt cttattgatt cgctttattc aaagatacca cgtaagaata gaaaaatgaa
    43441 tacaagtagt tgctaatcaa atacttcgat atttttcttt tttatcaact cttaaatgtt
    43501 caatccgcct gtgttggtct tgctaccatc tactattaaa atgtttatta ttgtttgccc
    43561 acctattttt tgattttagt ggtatcattt ttcaagttca tgatgaaatt cttatcatca
    43621 gtaaatgctc cagaaaaata aacaatgatt gaaaaatcag ttgaaacttg accaccgttt
    43681 attttctccg tattcttaga atagaaggga gtctgattga tgtattcact aatgaaaaaa
    43741 atcattaccg aaaaagatat tcgacgtcat gtttctttag tagaacaatt gctgaatcat
    43801 acaaagataa ccgtaaatga attagctgaa atcataggga caaccgaacg aacgattttt
    43861 tctgaccttc aatctattcg ctctcagctg cccgaaggct gggacatcat cagcgatcaa
    43921 gcaggaatca gtttgcaaaa ccagcaaaat ttattaacaa atgacctttg ggagattttt
    43981 ttcaaacaat cagtcagtgt cgaactgctg aaaaatctgc tttttacaaa aaaagttgct
    44041 gtccctgatt ttctagccga ccatgggctt tcttatggga cattgaaaag acatgtgact
    44101 aaaatcaatc agcgtttagc aagttacgac ttgcagatcg atttgacgaa atatacagct
    44161 tgtattcttg gaaaagagcg tgcgattcgt actttttatc atcgattgct gattcctttc
    44221 actcataaca actatttttt cgaggattat tcgatccatg aatcccatta ttttcaattt
    44281 ttaagaaacc tcagtcaaac tgaactagcg gttgaaacag aagagatttt cggaacatgt
    44341 tggttcttta tcaatacgat ccgtataaaa gcgaattgcc gattagattc ttctatccat
    44401 accaacagca cactctcctc cctgtatgac tctgcattga aaaaattgta tttaacggaa
    44461 ggtatttatc ttaaagacac tgagctttct tttgcttctt tctgctttct ggaaagttgg
    44521 aattacaaca ataactatgg acaagaaacc gctcgctgcc tgcatcattc tccattttta
    44581 gaggtcctcg aaacgtttgt agaggaacta gcttcagagc tgtctcttga tcaactaaaa
    44641 aaaacatctc tgactgataa cttggctctc ctcatattaa aatatcatga atcgccgata
    44701 ttgattgaag cattggaccg tcaataccat cgttttttag aaacatatga tcagcaatat
    44761 gcacacttgt atcaacagaa agagaattta ctgaacgatc taaaaaaaga aatagaaatc
    44821 aatgaaccaa attactttct ccaactcttg tctctactga tccaacaaac gattttttct
    44881 atcaaaccga atttgttcaa tgtctatttt attttccaag gagaaccttc atggaaggcg
    44941 ttcttgcagc aggaattaaa agattactta ggaaaacgtg tctgcttctt acctatcgaa
    45001 ctaaccgatc tttccactat ttcttttgaa aaaacagata tcttgatttc caatttccct
    45061 ctagattctc tagatatccc tattgtatac atttcttcta ttccaacgaa aaatgagctc
    45121 aatcgtttga ctgaactcac tttcaaaact tttttatagt aacggctagc gactgtattc
    45181 tctagctgtt ttttcttttt tgcgaaacag tttgttcatg atcccttttt ctatttctac
    45241 aactaagaat agaagaatca catttgctcc aatcaataac tgttgtgtca tcgttaaact
    45301 tactgtaccg atcagctgct ggccgactgg tagaaaaata accatccctt gcaataaagc
    45361 aaggatacct aatgaaagaa aaagcgcttt gttttgcaaa agaccttggt tcaatgacgg
    45421 cttggacaac tctctgcaat tgatcatgta aacagcttga gctaagacga tattttgcag
    45481 aagaatggtt tgctgtaagc tttgcccttc aaactgcata gccaataaat aggcaggaac
    45541 catgatcagt agggagacat agatgatccg aaacaaatga taaccagata ggattccttg
    45601 ctttggatct cttggtgggc gcttcatgat ttctggatct ggcgactcga accctagtgc
    45661 ataagataaa gtgattgtcg ctaccatatt gacccataaa atctgtactg gggacaaagg
    45721 tagcgggcga ttcagcataa gtgcccaaat caagatcaat ccttgagcta atgcagttgg
    45781 caagaagaaa ttgatggttt tttgcaaatt atccaaaatc cgccggccct cttttacggc
    45841 ttttgcaatc gtatggaaat tatcatctcc taaaaccata tcagctgcct gtttgcttac
    45901 ttcactacct ttgattccca tagcaattcc tacatcggat cttttcaaag caggtgcatc
    45961 atttacgccg tctccggtca ttccgacgat ttctccattt ttctgcaaag ctttaacaat
    46021 tcgcagttta tgttctggtg tcgttcgagc aaagacatcg actattggca cttgctgaat
    46081 caactcttca tcagacataa gatccaaatc gatcccttct aaaactttgg cagtatgttt
    46141 taagccaatt tgttccgcaa tcgcttttgc tgtgtctttg tgatcccctg taatcatttt
    46201 cactgagatc cctgcttgtt ggcattcctt tacagctttg actgcacttt cttttggcgg
    46261 atcgatcaat ccggcgatcc ctgcaaaagt taattgggtt aaagtctcat gcgtgagttc
    46321 ctgtttcgaa tctacttttt tataagcaaa tcctaaaaca cgctgacctt ttctcgcaaa
    46381 ttctgcagct gtctgactcc atgcttcttg ttctgcagaa gaaagagttg atttagcaaa
    46441 taatacttct ggtgcacctt tgacgtaaat caccgctcct ttttcctccg catgcatcgt
    46501 tgccatatat ttgtattcag aactgaacgg aatctttgct tctatttttt tcaaaggtag
    46561 attggctgtt tctgtgtgtc gaatcagcgc aacttctgtc ggattgccac ttaaattttc
    46621 tgttttctgt tcttcttttg tttttaattc ctgacagttt ttcataatgg acaacacgca
    46681 ggcttcttct tttgcagcta catccacaac tgtcatttca ttttttgtca atgttcctgt
    46741 cttatctgaa cagatcactg tcatagaacc taatgtttct accgaaggaa gtcctttgat
    46801 gatcgccttt tcttttgcca tttcatgtac tccaacggat agaatcatgg tcaaaacagc
    46861 tggcaatcct tcgggtacca ttgacaccac caaagcaatc gtagccgaaa gaaggatatg
    46921 ccaatccata ccatatcgaa atgtcgtaaa aataactaag aataggctca aacaaagaat
    46981 gccttgaaaa atttgtttgt ttaatcggtt gattttttta atcagaggcg tcgtttgttg
    47041 ttcgaccgat tgcaatgctt gattgatttt accgatttct gtcgtgtttc ctgtttctac
    47101 aacgacaccg actgcagaac cagattgtac aagcgttcct gaaaaagcca tatttttctg
    47161 gtcgccgatc gattcttctt ctgtcaaaac ggcatgactt ttttcagatg cttctgattc
    47221 tcccgtcaaa atcgattctt ccactaataa atcatgtgct tcgattaatc ggatatctgc
    47281 tggaacgaca tctccggcac gtaagacaat gacatctcct tttaccagtt ctttcgcagg
    47341 gattttttct tgcatcccgt ttgatagtac tatcgcttct tgtcccatca gctgcttcaa
    47401 accattcaat gattcttccg cttttctttc ttgccagtaa ctaacgagtc cattgacgat
    47461 cactacgaat aaaataatac ttccttcaat atattcacta gaagcaaatt tcaaaaaaga
    47521 ggcaatgatc aacacgatca tcagcagatc agtaaaatgc tttgctattt ttttccatcg
    47581 ttttgtctgc tgccttgctt ttatttcatt atatccttga tcagtcaggc gtttctgtcg
    47641 ttcttcttgc gacaagcctt ctgttgatgt ttgatagttt ttcaatacat cttctaccgt
    47701 caatctgtac cagttcatcc tatcccctcc tgtttaattg atactgttat tatacgcagg
    47761 gaagtttttt caggcgtatc gattatttca ggggggtctg aaataattcg ttttgcaaaa
    47821 tgtatctggg cctaactgat atttctattg cttattcttt tatatacaaa atttaagaat
    47881 acaaaccaga taaccaataa aaaaggaata aagcgtgtcg tgctttcgaa taacaagctg
    47941 aaagcaattg aagtaaaaaa caccaacaaa gcaagatctt tatatctttg tgtagtgttc
    48001 cgtttctttt ctttaataaa ttgaacatgt gcacacaaga tcgaagacca cacaaataga
    48061 aaacaactcg ttgtcatgcc tgccaaaagc tgaaaaaaca atcgactatc agctacaaaa
    48121 aaatgtagac aaagaccggc aaaaaataac attcctgtaa ttaaaatacc gtgtaaaggt
    48181 acgcctttct tagaaagcgc acaaaaccaa tggggcaaac ttttttcttt tgctgtttga
    48241 tataaaatcc tgctagtact gtagactgca ctatttgctg aggacaaaga agcaaccaaa
    48301 atcattagat tgacaattgc tgctccgcct tggcaatgga tcacttgcag catagtcact
    48361 aaaggacttt cttttaaatc taaacaatgc catggaacaa caaataaaat cgacaaaatc
    48421 gttcctatat agaaaaagag aatacgccaa ggcagacagt tgattgcttt tttcaaattc
    48481 ttttgcggat cacttgtctc gccaacggtt aagccgatca tttcaattcc agcaaaactg
    48541 aataaaacca taggaaatga gactaccatt ccagcaaagc caaacggtag taattcatcg
    48601 atctgtccaa aattttcttt gattccgata gactgggtat gaaaatctga gacactcaat
    48661 cctataccca ataagataaa aaggaaaatc cccaagatct tgatgattga gaaaaaagat
    48721 tccaattcac caaaccactt gactgcaaca aggttgactg ccattaagat cattagtgcg
    48781 ctacctccgc ttatcaaagc tggtacatgt acaaaccaga aatggatata caatcctatt
    48841 gctgttaatt ccgcaagtcc agctccaagc cagcaaagcc aataagtcca attgacaaaa
    48901 aatgcccact ttttgcctaa ataatagtca acgaaatcac tgatagagtt aaatgtacta
    48961 tttgctaaaa ataaatcacc catggcccgc atcaaaccat aaatagccaa accgcatatc
    49021 aaataaacga gaataacagc tggccctgct gatgcaattg cctttgctga acctaaaaat
    49081 aatcctgttc caattgcgcc acctaatgcg attaactgga tatttcgatt agttagagtc
    49141 cgtttcattg ccggtttttc catcaagttc acctttttct ttttcaagca gcatggcgac
    49201 ttcctgttcc caagaaggtt tctttatttt ttcaatacag agtggtacta caaacaaaat
    49261 gacactgatg gctgtgactg caattagatt gatccatgtt ccagctttca tcgtcagtcc
    49321 gacagaaaat ccgattgccg caaataaaac aataacgaca gtccaaactc caagattgct
    49381 ctttttctct ttttttccaa ttctaaatgg tcgattcaac tttgcatgtg tataacgcaa
    49441 tctcaaaact gcgatcccca tgatgacata gacgatacag tagataacag tagttgaatt
    49501 tacgagcatc aaaaatgctt gattgacgcc aggaatcagc aaataaaaaa gtgcaaagat
    49561 tgagataatg acagactgag tcaaaataac ggatttggat acgtcaaatt gattcgtctt
    49621 ccaaaaattg aattttggtg gatacgcacc ttttctagca ctttctgtca ctgtctttgc
    49681 tggtcctgag gcccaagcag ataattggac tgctacacca atgaagacaa gtaaactaaa
    49741 cagattcaca ataagatgtg gcaaaccaag aatttggcaa taaatcgaaa tagattgcgc
    49801 gatattattc aattccattt gtccttttgg tacgacattt gctacaacta aagcattcaa
    49861 aatattgacg aaaaatataa aaattaacgc ggcaaatact ccgcgaatat acgttttaac
    49921 tggattttcc aatcgagtga tatacacaga ggacatttcg ataccagtaa aaataaacat
    49981 gatcggcgca aaataaacaa atgaggatgc agtcgtagta tctggtatta atttgtccca
    50041 tgaaaaactg cctaatgttc cggttggtac gatcccaact ttgatccacg cagcaaaccc
    50101 taataagagc atcatcgtca aaggcacgta caatcctaac caaacaccga cttttccgcc
    50161 gatttttgcc atatcaaatt tcaaattcaa aagagtgatg atccaataaa cgactaaaat
    50221 gacaactaat gtaaatttgc tgttcaaacc aagcggcaca ttatcgataa tattaccaaa
    50281 caacggagcg agtgctgaag cgaccatcac catcccagga aacatttgga cccataacag
    50341 ccatgagaca acgaaccccc atttttttcc tagagcattc gatacccaaa gttctggacc
    50401 acctttttgg ggaaacattc cagcaaattc accagagatc aatgagattg gaaaagcgta
    50461 caataatact gcaaccagca tgtaaaaaat catcgtccag ctcgttgctg ctacatctgg
    50521 aatatttcgt acacttgcta caagggcgca ggtcattccg ataaaggtca tggtcgtgat
    50581 ttgctttgat gaaccagtat ctttcatctt tattccttct ttcaatagcg acatatgttt
    50641 ttgttttctt ttatttttgt tcaagaacag aaatgacaag gtttcatcga ttcagtacaa
    50701 aacccagtat caccgaaaca tccattgaac gatcttagtg aactgggttt gatacatgaa
    50761 acgaacgatc caacttatag aacgttttat aaaaaagaat atcttggcga ggaatatatg
    50821 aaaaacaggg ggaaatacaa ttgttttgat gaagcaaata attgtctgtt ctccatatgt
    50881 ctcttgttat tctttcacac aaaaatcagt tctattggat gatttcgttt agtttctttt
    50941 ccattgcttt ggcgattttt tttgtataaa aattgaaaat tcgatcatca ttcaaataag
    51001 gagtcatgat agccgcacgt agcagagtga ttttctgttc ttttttccat tcttctttcg
    51061 ttagacccat tctttcaaca aaacgaatcg gagaatcacc gtaatcttct tgtgtaaatg
    51121 tagtatgaga agtaataaac cgttcaccat aaacatcgcc atccatataa gaagaccgat
    51181 cgaacatttt ttcatttaat tcattgatag cgttcaaatc tgtacagcct tgttctttga
    51241 atacccaatt gaccatatta aaatcaggat gattcaatgg atagacttcg attgtttttc
    51301 ctttaacagt aaaagtcaga tgatcgagaa attctctgaa tctttgagct gcctcgattg
    51361 aagccccaat caattgacca tatccggtca cattcaatgg caatacacga tgtgctgtcc
    51421 agactgcggc ggcggttgct ccagcttttg atccttccaa aatataagct cctaacatat
    51481 ctggagcctt tactgatttt tcaaacacat atggcgcgaa ataagaaatg atatttctca
    51541 tatttttatg tttgattacg attcctccag cagcataagg gacatagccc atcttatgag
    51601 gatctatcgt gacagaatcc gcttctgaaa tagctctgaa cccttcatat acctctttgt
    51661 caatcgttac gcagtggtgg aaaacatgat gttcttcaaa gaattcagct aaggaagcat
    51721 acggcacaaa ttctcctgct tcgtttagaa acagtgaacg cgcatagccg ccatatgcgg
    51781 catcaacatg taaatagaaa tagatccctt catcttttaa cctttctctt aattggacga
    51841 ttttatctac actgtctact tgaccttctt ctgtcgttcc tacgacagca accacaccta
    51901 gaattgggat tttttgtcca gctaattcac gaattgtttt ttccaaagca ttaatatcca
    51961 ttcgataatc ctcttgaact gggatagcaa ccatctgatc taagccgact ccgcaaatat
    52021 ctaacgcctt catccaagaa tagtgttttg tttgagggac cagccatttc cctaatcttt
    52081 ggatattttt tccgctacgg gaagaagctg ctttgacctc atccatttct tcgtccgtaa
    52141 agctttcggt catttccaaa atttcttcaa cagataaatt caaaagttcc cactcagaca
    52201 tttttttcac tttttcaggc agcacttcct tgacagctaa aggaatcgat ttgatgcaac
    52261 gggcgtacca taatccttca agattagcaa tagaaccatc tgctgcaatg tgtccccatc
    52321 cgtctgtcat attgaataat gaagcaaaat cctgtcctac ttctgcttcc atttgcgaag
    52381 ttgccataga agattccaat gcaacattat ttgggttcca cagcatcgca tagttgtaag
    52441 ctaataaagc aggcatcaat gtttcagcat tcatttgtcc ccagtaacgg cctgctgaat
    52501 gccatgggat cgatcctgcg cgtaatcgct gactgacttc atccaagacc tctatcatat
    52561 ggtcgcgggt ggctagataa tctggagtga gcttatctcc ttcgctaatt gcaggcatat
    52621 ctgaaggaat ataattttct ctccatccaa gatgttcatc taccatcttg ttcaacaaca
    52681 ttttataaac tggtccattt tcagctttgt cgcctataaa gacggccttg atatccatat
    52741 ctttcatttt tcttccctcc atattttgtt ggtcaatgtc ttgcagatac atgtgtttat
    52801 ctgtaacacc tcttcactat agcgccaaaa atgagaaatt tttttttata acatcaaatc
    52861 cacgtagatt tttacaaaca aaaataaata aagtcggatt tttctgataa ttcaacgcaa
    52921 aaaagcgaac aatttttcaa taaatcagtc ttctaaaata gataacctaa aaaaagcgaa
    52981 caaacaaata aaatgactgt ccttcattca ctaaacatat ttttattttt ttatattgtt
    53041 aaaaattcac aatttatttt ttaatagaat tatctgtttt ccaaatttta taacataaaa
    53101 aacctttttt aacggcattt atatgctttt tcagaaatta actgatgatt tcaaaaagaa
    53161 aacatgtcat aatatttccc atattgtgca aaaaaataca aggcggaggc tctactatga
    53221 ttgaagaata tattgagaaa aatattttaa gacagctgtt tttatgtggg caattctatg
    53281 taaataaaga agtcaatcta gaaaagctct ccaatctcct gcatgtttgc aaaaccactt
    53341 tactcaatga tatcaataac ataaaaaaag aatttgaaga acagatcgct tatacacatc
    53401 gggaaaagga ccgttacact ttatattttt cagaacatat acctcgatgc aagatcatgc
    53461 agcaattgtc tcaaaattcg ttgtttttaa aaacttgtct gctttatttg gaagaagatg
    53521 aacctgatta tcttcaatta actgaatgtg aattcatctc tgtcagcaaa gcttattcat
    53581 taaaaaaaca agtattagct tactttaatg actgcgggat cgaaatcgac cgctactccc
    53641 ctcgattcac tgaaatggaa agacgtctgc tgcttcttaa tgtgtcgtat cgtttaggcg
    53701 gttttaattc ttgggagcta cctgaatcgt tttttgaacg tgccgatcgt ttcattgaat
    53761 ccgtaacgga aaatagtggc agattttatg ataaagaaaa taaagaaatc ttatcgattg
    53821 gttttgctat cagtttccta cgccagcaag tgtgtgctgt gacaatagat tcaaaattta
    53881 tcgaagaaat caaaaaacgt cctatttaca attatgtcga atcagcatgg gaaaacactg
    53941 atttccaaac ctattataaa aaagaagaat tcgcctttat tttgacacta ttcaatcttt
    54001 gtaattatgg tttccattct tatcagttga ttgcggaaga ctttcaacag ctacaccaag
    54061 tgtttattga caatactccc gaaataaaag aactagttgc tacctttgaa tctcacttca
    54121 atcaagaatt attcggaaac caaccgttcg aacgtgcgct gattcatctg atgcgttcgg
    54181 cttgggataa ctatcaatta ttcatgccag aaaaatttta tcttttaaat gaggaacaga
    54241 caaatttata taaagaagtc cagacaattt tttccagctg gtccagccag ttaccttatg
    54301 atctacggtt gaatcctaat tgcatgcggg cttttgtgat cgaattgagc ggaattctcc
    54361 gtctgacaaa agaacatctg actatctata tcgtcacaaa ttcggatgtt cattatctga
    54421 tttatcggga agccttggaa gctgtaacta ctttcgattt tcaagttgca ccaacaattt
    54481 attcaagtat atctgatata aagaagtatg cgcagcagtc ctcaaaccgg gtattatgtg
    54541 aacgtacatt atatacacca gatgcggtcc aatatgaaaa tattattcct atttcgatca
    54601 atacgatcga tcgagctatc atttcagctg tgcaaaacaa ataaggggct gtgacaaaaa
    54661 tcatgtcaca gcctcttatt tgttgaagat gcccatttct gtaacaatct cttgatacac
    54721 gtcaggctca caacctcact tattttggac atctaggggt ccgatctctt tatttaaaaa
    54781 ataagaaatg atggttcgga taactaccaa cgccgctagt tggaaaatat cctgcaaggt
    54841 aggtttgata atcgattcga tgatcccagc tgaaacaaga atttccaaac ttagaagtat
    54901 atagcctccc aacatctttt taatagcatt gttctcgctt actcggtcgc tttgcacttt
    54961 tgtatggaca aataaatatt tcaaaaaatc cttgattgat agacaaacac cccaaaccaa
    55021 taaacagata gcaaagatat caagtcccag tacaagtttt tcgaaatatg gaagaagaaa
    55081 ctgcatcaac tctgtcgtca tcctacctct cctcttcttt gtttttcttt gtcttctatt
    55141 tcctgattca agaaataaga aatgactgtt cgaatgataa taataagtgc aagcttcaaa
    55201 atatctgtcc aagtcggctt gataactgac tcgatgatat cagcaacgac tagtacttcc
    55261 aagcttaaca aaatataact tgctagcagt tttcttgtat ctgcattacg agtattacgt
    55321 tctgcatagt ttttcttgac atgagaccaa tcaaaaagtt tgaatagaac aagaacgatc
    55381 ccgacaatta agataccgat agaacaaata tttaaaatag caatgatcca ttctagaaat
    55441 tcaattaaat ttgagcgaaa catatacgct acctactttc ttttaatttg ttcctattct
    55501 tttattgtaa aagaaagtta ccttgttcgt ctatttaaaa gttgttctaa tgataacggt
    55561 tcgcttaaat ttttcttttt cactctccca tttttctccc tccatccata aatcgtggaa
    55621 gtatccaaaa caacgtagca agttaaaata aggcttagtc cgtcatcagc ttttcaaaga
    55681 agcactgtct cggtgctttt tttgtaccca aatttctaaa aatagaacca tacacctcct
    55741 caaatctttt cttcttacct accctatttt tatttacagg aaaatcagac acttcttctt
    55801 ttttgtcaaa atttcctttt tcttctcgtc ctatcatttg gcattctaat aaaaacgaac
    55861 tatccttgga tatagaaaga gaaaacaagg aggcgttttt catgagtaaa aaattaatca
    55921 aagtcggcat cggtttaggt ttgcttgccc ttggcgctgc atatctagga aaaaagacag
    55981 gactattcga agatgacagt catttatatg atgaattcga atcaatctag catcctattt
    56041 agttgaatca ctacggacat acttggaaga cattggagga atcacaatga gaaaaagtaa
    56101 agcaataatg attggtgccg gtattgccaa tatggcagcg gctgtttatt tgattcaaga
    56161 aggcaaatgg aaaggggatc agatcacctt ttattcattg gatgatcacg gttcaaatga
    56221 tggtgcaccg accgacactg tagtggatga atattggaat aaaaaccatc cattagaaaa
    56281 ccaaaaaggc tatattgctc gaggcggtcg catgcttaat taccgtacct atgtagattt
    56341 gatggatctg ttaagtcgaa taccttctgt tactgagcca gggatgactg ctgaagaaga
    56401 tacgagagat tttgatgcaa aacaccgaac ctttgacaaa gcacgtttga tggaaggtgg
    56461 caaaggaatc attgatggcg gaaaactggg attcaataat aaagaccgta ttcttctgac
    56521 taaactgatt gctatgcctg attcagaaga agaaaaacta gacaatatca ctatcgaaga
    56581 atatttcaaa gatgatccac acttttttga aacaaacttc tggtttatgt gggaaacaac
    56641 atttgctttc agaacacgta gttctgctca agaattgcgt cgctatatgc atcaaatgat
    56701 ttatgaattc actcaaatcg aacatctagt gggcgtcaat cgaacacgtt acaatcaata
    56761 cgaaagtatt atgcttcctt tgatcgagta cttgaaagaa caaggatgca aaatcatttt
    56821 gaaccgccgt gtcactgact gggaattcaa agaaacaccg atgcaagatg aaatcactgt
    56881 gactggtttg aaaattacca atacactgac tcaagaagaa gaatacgttg tggtcgatga
    56941 agatacagca gttattttca ctaatgggtc cattacagat tcagctactt taggagatta
    57001 cgaaacacca gctgtagaaa atatggatta tggtgcagct tctagtctct ggaaaaaagc
    57061 cagtgaacgt ttctacaact tgggaaatcc tgacaaattc tttgctgatc gagatgcaag
    57121 cgaatgggtc agctttaccc tgacgacgaa aaaccatttg ttgttgaatg aaatcacccg
    57181 tatcacgact caagaaccag gaaatgcctt gaattcattt atttcaacca caccgatcac
    57241 gccgttaggc caaaaagatg tcaatatgtc tattgtcgtg catcatcaac cgcattttac
    57301 gtctcagaaa ccaaatgaaa cagttatttg gggttatttc ctttatccaa gacgacgtgg
    57361 cgaatttgtg gataaacagt atattaaaat gaatggaaaa gaaatgctag aagaactgat
    57421 tggacaactt tcaaaagtag atcctggacc agtgaatatt cgtgaaaaag aaacagaaat
    57481 tttcgacagc gtaataaata atatccctgt ctatatgcct tatgcttccg cgttgttcaa
    57541 taatcgtgcc aaaagcgatc gaccaaaagt cattcctaaa cattcgacca atctggcctt
    57601 tacaggagaa tttgtcgagc agccttatca gatgatcttt actgaacaaa gtgctgtacg
    57661 ttctggagaa atcgctgctt tccattttgc gggtattcct atgtcccgtc ttgtcaaaac
    57721 accgcgttac gacaaagata ttcctactct agctcgtgcc gcaaaaaaaa tgtttgaata
    57781 atcaagaggt tgtgaaaaag ctgccctgca tcaagtaata agaacaacca ggctaaaaca
    57841 gcttcttatg tttttgattt ggttggactt attaccgcag atgcaagctt ttgaacaccg
    57901 tttaccaaga ggttatgaaa aagctgccct gcatcaagta ataagagcag ccaagctaaa
    57961 acagcttctt atgtttttga cttggttaga cttattaccg cagatgcaag cttttgaaca
    58021 ccgtttacca agaggttatg aaaaaggcgt ttagtttcga gtatgaagaa aaattttgtg
    58081 aaaatggttc ctcacgtttt tgcataaatt tgacttaata ccgagaaact gatcccttct
    58141 gtcataacct ctttttattt atgtctgttt ctttttgaaa acaactattt tttcaacgtt
    58201 tactctcttg ccaatccaac atcatctaac ggatacaact atttcaatga ttaccattac
    58261 actaaaaaat cggaaacaca cctctccgaa cacaaataca taagactata gaatgtttca
    58321 tttaagaaaa aaagcgtttc attatcaaaa aggtaagatc tcctttaaaa tgaaattatc
    58381 tgtagtacta ataatgcaga caattttatg aatctgaaag gagcatctcc aatgaaagca
    58441 gtcacttggc aaggaaaaca gaaaatggaa atcagaaacg tagatgatcc aaaaatatta
    58501 gaaccaacag atgcaatcat ccgtatcaca gcaacagcca tttgcggctc cgatctgcat
    58561 ctttaccatc atggtgaagc agttttggaa aaagggtatg ttgtaggaca cgaaccaatg
    58621 ggaatcgttg aagaagtggg atctcaagtt caaacattga aaaaagggca acgtgtggtt
    58681 atccctttta atattagttg tggtcattgt catttctgcc tgaatcacat ggaaagccaa
    58741 tgtgatgaat caaatccaga tcagttatat ggcgggcttt tcggatttgg caagttgaac
    58801 ggtaatcatt ggggtggtca agcggaatat ctaagagttc cttatgcaga ctctacttca
    58861 tttgttgtac cagataatga tcttcctgat gaaaaagttt tatttctatc agatattttg
    58921 cccacagctt attggagcgt tgaaaacgca ggagtaaaaa aaggcgatac agttgtgatt
    58981 ctaggttctg gtccagtcgg tctattcgct caaaaattcg ctgttatggc tggagcagag
    59041 cgcgtaatcg cagtagatcc agtacagcac cgtctagata aagcggctaa ttacaatggg
    59101 gttgaaactt atttcttaga agacacggca caagctggaa cagaattgta tgaactggct
    59161 aaaggtggcg cagatgtcat tatcgattgt gtaggaatgg acggattaga gcctgtcaaa
    59221 gaaaaagcga aaaatttagt aagcttacaa agcggcacaa tctctccatt acagatggca
    59281 tcgcaagctg tcaaaaaatt tggaacagtc caagtaactg gtgtttatat gactccagct
    59341 tcttcttacc cgctgcaaga attttttatg cgggatatcg ctgtcaagca tggacaggca
    59401 ccaattatcc atctaatgcc aaaaatttac gcaatgattg cagaaggact attcgatcct
    59461 acacaaatca ttactcattc gctgccacta tcagaagcag cacaagcata tgagatcttt
    59521 gacaaaaaag aagataaaaa tataaaagtt gttttgaaac cttgaattca gtaaaaaata
    59581 caggtgattt tctttatgcc caaaatcaaa cgaaataccc atgagatcga aataggaaaa
    59641 gaagaagata tcgaaatacg aggacgtcgc ttccgcttct atttccaaaa tcgttacaca
    59701 ttgatctcac tggctgtcga tttattaacc gggattttct atatttttgg aagccttgct
    59761 tccttaacta aaatccccga tcgatatggc acctattttt atttggctgg tgctattttc
    59821 ttgacgattc gtcctattct cagaatcgta aggaacattt ttatttacga cgaaaaaaaa
    59881 gcaatgaaga gaacaagcaa aaaaaaagag gcatgagaat ttttcaaatc tcatgcctct
    59941 tttttcaccc tatcgtatca ctttgataca gacgagcggt cgtcccactt tcttccttga
    60001 ccattgtcaa aaatgtccac tcgtatcgtt cataaaacgt ggtatgatct gtaattaagt
    60061 aaagtttttc gtacccaagt tgatgcattt cttttctgat ttccgctaat atccgcttgg
    60121 ctatcccctg cttacgcttt tccggttcaa caaacagggc acaaagattt ggtgtcagat
    60181 cagggcggtt atggaagtcg ttttcaatga cgcccgctcc tccgacgatg gtttcttttt
    60241 catctaagac cacataccat tgaggaatac tttccttatt agcgattcca gtacgaatac
    60301 tttcctcata cacaacagca ggaactcccc attttgatga aaaccaattc gcgctttgtt
    60361 ttactaattc tgggtgttct cttaatttga tccagttata tgtaaccaat gtttaaacct
    60421 ccgactaaaa taaaacgttt acaaaaatta tacaagtctt tatgaaaatt agcaatgaca
    60481 acaatagtga tgtaaaaaaa aaaacggaaa acaatcactt gttctccgtt ttttgattaa
    60541 tcaagcatct aaagcttctt ttcttctttc ttggatcgta gctaccttga cgaacggtag
    60601 atagatcaaa atagaaagaa ccaaattgac agcggctagt acaccacctg caatactttt
    60661 tgttgccaat acaccgccaa taattggtgg agttacccat ggaggcataa cggtagctgc
    60721 tggcactaaa cctgtagaag tcgcaaagta agcaacacta actaagacca ttggtgtcaa
    60781 aataaatgga atgaacatga tcgggttcaa cacgataggt aacccaaaca tcgttggttc
    60841 attaatattg aagacacctg gcgcaatcga caagtttgtt acaaccatat atggtttgtt
    60901 tttacgtcct actaaataaa tagctaacag taaacctaaa gttgcacctg ttcctcctaa
    60961 gttgacaaag ctgtcaaaga aaggtttgtt cacgataaac ggtgcgctat gtccagctgc
    61021 gattgcatta acgtttgctt caatggctgg cgcattgatt gtttgcatca aaggatcgac
    61081 catattcgct ccgtgtaaac caaagaacca taagaatggt gtgataaatg caagcagtaa
    61141 ggcagatgga taagagtttg caagtcccat aaatggttct tggaccactg tatagaaaga
    61201 acctacgata tcatcaacac cgaatgcagc aaaaattgca gcaactaaag caaacgcaga
    61261 gatagtgatc attgctggta gtaatgaagc aaatgctttt gcaactgccg gtggcacacc
    61321 atcaggcatt ttgatgatca atttttcatt tccgcttaaa cgtacaaaga tttctgttga
    61381 aataatccca acaataatag caataaacaa accgtttgag ctcataccta atgctccgcc
    61441 taaagcgaag aaagagccta aagaaatcac acctgcggaa acaccgtctt ttcggtagcc
    61501 gttagctaaa tgataagcaa gcaagaaagc aatcaagaca gaaagaatgg caaatgtacc
    61561 gttccaagca gcagccccga atcctttcca tgattcacca ccaaaaattg agttcatcaa
    61621 ttcttggaac ccgggaatcg gtaagttatt gatcattact gctaatgctc ctaagatcat
    61681 taacggcata ctcaccataa atgagtcccg aatcgcaact aaatgccgtt gtgcaccaat
    61741 ttttgatgct acaggtataa aatgtttctc cataaatgca acaaatttat ccataaatct
    61801 tccctctttt ctttactgta gattacgcgt tcaatcgacg atacagatta atgatctctt
    61861 ttgctaaatc tgtaaaagct atagatgtca tcagatgatc ttgactgtgg accgtcaaaa
    61921 gcgtgacttc aacgtgattt ccttgtgctt cttgtgtcag catctctgtt tgagagtgat
    61981 gtgcttggac taaagattcc tctgcatctt tgattttttc atctgctaaa gtgaaatcac
    62041 cttttttcgc agcagcaatc gcttccattg catcgctttt tgcatttccg ccaaacatga
    62101 tcagacccat gatggcttct agattttttt gatcttccat ttattgtatc ctccgataat
    62161 ttttattatt tgtttattag tttttctgct tggtcgagta ctttttcacc attcatcatg
    62221 ccatagtcag ccatattgat tacatctaaa ggaatgcctt taggtgctag cttttcagca
    62281 aattgatttt gcataaagcg tacttgcggt cctaaaagta aaacgtctac atttttagat
    62341 tctagttgat tatccgcatc tgaagcagat acagcaaaga tgtctgcttc cattcctttt
    62401 gcttctgctg ccttttgcat ttttgtgact aggagacttg tactcattcc agctgaacat
    62461 acaagcatga ttgttttctt ttccatttta aacacgttcc tttctatact tacacttgta
    62521 tataagcaag tagcgtgcca actttaaaag cgctttaaaa agttgatatc atcacattta
    62581 aggtctatac caagttgcat acacactaaa aaagaaagcg cacactaaac acacaaatta
    62641 atttttatta ccttttttct tttaacgtgc acaaaaaaag ttgtgacaca aaacttgtca
    62701 caactttttt caaataaacg gtattcaaaa gcttgcatct gcggtaataa gtccaaccaa
    62761 gttaaaaaca taagatgttg tttttacttt gctattctta ttacttgatg caggacagct
    62821 ttttcacaac ctctcaataa acggtgttca atcagctgac cctgggtatt agctcaaaac
    62881 gctggattca caacctcttt tatttcaaat aaccagtaaa tacaatgaac atctacaaaa
    62941 aacttctatt tatttataga tttcgtcagg caaatccaat tctctcttat caatcaaagt
    63001 ctgataccac gaagcagatt ttttcaatgt tctgcttcgt tcatcgttca attcgattcg
    63061 aactaatcca tatcgattct taaatgcatt cattggtgac acacagtcag tgaatgccca
    63121 taacatatag ccttcgcaat ttgcgccttc ttctgttgct ttgatcaagt aatgcaaatg
    63181 ctgtccgata aattcgatac ggtaatcatc ttgcacgatt cctttctcat ccatatactg
    63241 ttcttcatat tctcttccca taccgttttc tgttatcagc caagggatat tatgataatt
    63301 ttcttttaga tacatggcta tatcatacat gattttcgga tagatttccc atcccctgga
    63361 attattcatt ttcttcccag gtaaatcaaa gacttcataa tatcgttcag gatggaaagg
    63421 tgtctcttca ttccattgat aacgaggggc gcgaacgcgt ttaggataat attgattgat
    63481 tccaaggaaa tctactgtat gatgctgaat cgtttctaaa tcctctgttg caggatcgaa
    63541 ataaatcgat tgtttttgac acaactccag gaactcttct gtgtagtatc cttttaccat
    63601 cggatcaaaa aatactcgat tataaaacaa atcatacata taagcagctt tttggtcttc
    63661 tggagaattg gatcgagcat agaccatttc aggatttaaa atacagccaa tccttccacc
    63721 ataatttccg ctgcggtaaa tctctactgc tttagccgtt gctaaaactt tatgataatt
    63781 ccacatcatc cacttttttg tgttttgttc atgcggccag cgaacagcat ccaaatagca
    63841 acgcgtttgg ggaacgattg gttcgttgaa tgtaaaccag ttttttaccc tatcaccgta
    63901 acgatcaaag gcaatttgag catacttagc ataaaaatca actacttttc ttgaagacca
    63961 gccatcatat ttttccaaaa gaaaagctgg gacttcataa tgttccaaac aaatcatcgg
    64021 ttctacgtct gcttcgatca aagagtcgat catttcactt atatgctgag catattcttc
    64081 atctactata cagttctcat aatcagtaaa aaaacgagcc caattgatag aagtccgata
    64141 atgtgtcaga tggatggatt tcatcagatc cacatcctgc tggtattgtt ccataaaatt
    64201 agttgctacg gctgggccat acccattatg ccaaacaaaa tgttcatttt tataccagct
    64261 atctataaac gaatcctgtc cttctttttt tcctttccaa ccttccgttt gccaagcaga
    64321 agatgcagca ccaagaataa aattttttgg aatagtaatt ttcatgattt cctcctgttt
    64381 tttagatccc atcattttcc acacgatttg ctacttttac aaatggcaga tagatcaaaa
    64441 tagaaacaat aatacaaaca agttgcgtaa taactgcacc gatatttcct gctgttgcca
    64501 gataagcatt gacgatcggc ggcattggcc aagggaccat tacaactgct ttcgccgcaa
    64561 atcctaaagc ggttgcaaaa taaccaatcg ttccagtgat aagaggcgtt aagataaacg
    64621 gaatagctaa aattggattc agcatgatcg gcataccaaa aataactggt tcattgatgt
    64681 tgaagatccc aggagcaagg gataacttgg tgatttgttt catatcttcc cgttttccaa
    64741 ctaacaatac tgcaatcagt aaaccgatcg tgactccaga cccgcccata ctcatataca
    64801 tatcccaaaa tggcatagta ataatgttcg gtacttcttt tccagcttca aaagcttccg
    64861 tatttacagc aattgccgct aaaagcaacg gttcccgtat cggcttcacc atttgatttc
    64921 catggatacc aatcacccaa aagacttgcg caataaacat caatagcaaa atacccggta
    64981 acccttgtac gacaccttct aatgggcgtt gtacgacatt gtaaatgata tcataaaggt
    65041 acattcctgt aagttctttg atgataaatc ccgcagccgc gatacccgat acagtcagaa
    65101 tcgtcgggaa taaagcagaa aaactagcag aaacattcgt tggcacggta tcaggcattc
    65161 tcagctgtag tttcttctgt ttcctcaacc agcaataaag ttcaatagaa aggatggaga
    65221 ccaacattcc caaaaataac cctttcgtat ctgtatattg tttagccaat acgttttcta
    65281 ctataacagt ctgattttca ttcactaatt cataaacagt tggattaaca gataaataag
    65341 acatcactgc gatgatacca gggaatacac catccacttt attcagcttg gctaattcga
    65401 tccctatcag aaaaacagca tagatggtca agaaacttag tgtgacatag ctgatggcag
    65461 atgcgatagg ctttaattct tctaaaaaac gcaagaaact tatctgtgct aagccatttt
    65521 ccgagtcaaa gaccattgct gaaaacaatg tgccaaaagc acccgtaata attaccggaa
    65581 tcaatgcagc aaatgcgttt tttatcacca tgatatacct taaattattg attctcgttg
    65641 ccacgttccc taaaacattg gctaatttat ccataaaact cattccagat cccctcattc
    65701 ctgcttcttc tccttgacgt catctctttt tgtctattag tcagcgctta catttcgatg
    65761 aacagaaact atttactcct cttttttacc aaatataaaa caaaaattca tctgatgtat
    65821 ttatcataaa atacatcaga tgaactgtca attagtttct ttttctccta ttattttctg
    65881 taattctctc cgatttattg ccatatggac agtcattgca tcaaacgcag caagtgcatt
    65941 ccgcctttcg atggcctgaa cgatctctgt atgaatatcg atcgtctcga ttttcagatg
    66001 ctggatatca taatttttat taaacaaagc aattgattca ttaatgattg ggataatatg
    66061 cagcaaggct acgtttccgc tagcttctgc aatcatcgta tgaaaatcga tgtctaattg
    66121 aacatgttct tgtgtgcctt ctcgaaaaga agtttcaatc ttttgcgtta attttttcat
    66181 ttggacgatt tgattcttcg ttgcatattc cgctgccaaa cctgccatct taggctccaa
    66241 taaatatctc agttcaaata agtctcgtat catccgatcc tgatcttcaa tcaaagaaaa
    66301 accgagtggg tcattggcaa tccctcgctt atcactgaca aatgtccccg ctccttgccg
    66361 aacttcaagg atattcctgg aagctagagc ccgcacagct tctcgcaccg tgcttcgccc
    66421 cacctctaaa taatcggaca attcatattc attcggcagt ttatctccta cttgcaactg
    66481 catttccttt aaatatctaa ggatcttgtc agtagttact tctactaggg ttttattttc
    66541 ttccaaacca catacctcct tttcatctga tgtatttttt cgtaattatt ataacacgtt
    66601 tgaatcatct gatgattttt tgtatacaaa attgattaat ccgttcatag acatacaaac
    66661 gaagaagata tttaccataa aaaagactag gataaaattc actcctagcc ttcgatcatt
    66721 tttattttga acgacaaaaa atatgatgtt caataacaga cgatcttcat tcgattcttg
    66781 atgtcatctt ttgttttttt taatatatgg ataaaaagtt tgattacatc gtttctattc
    66841 tagaatttgg tcgattctca attcttcttc tggtgagaaa tctaaattat ccatcattcg
    66901 aacactttct tgtaactgac ttaaccgact tgctccgacc agtacacttg taacagattt
    66961 ttgtcgcaaa ttccatgcaa gggccatttg cgctaatgat tgtcctcgtg atacggcgat
    67021 tgtttgcagt gctttgattt tttccagagt tgatccaact tgttcttctg aaaggaatgg
    67081 tatttcctta cgatgtgctc tagaatcttc aggaatacca tgtagataac gatttgtcag
    67141 caacccttga gcaagcggac tgaacgtgat tgcccctagc ccttcttctt ctaatactgg
    67201 agatagatcg tcttctagta gtgcacgaga aaacatattg tagcgcatct gatgaataat
    67261 gaacggtgct tcctttcttt ttaaaatttc agtcattttt tttgtatctt ctccattata
    67321 gttggatata ccaatatata atgcttttcc ttgtcttaca agttgcatca aagcttctgc
    67381 tgtttcttcc aaaggtgtat cagggtctgg gcgatgatga tagaagatgt caacataatc
    67441 tagccccatt ctttggagac tttggtcaca acttgaaatg atggatttac gagagcccca
    67501 ttccccatat ggcccttccc acatatgata acctgctttg ctagaaataa tcagttcatc
    67561 acggtaggct tgaaaatctt ctctaaaaat acgcccaaag tttttctctg cagctccagc
    67621 agggcctcca taattattcg caaggtcgaa atgggtaatt cccatgtcga atgcgcctct
    67681 taaaatctct ctttgattat ggataggatc ataatcacca aaattttgcc ataatccgag
    67741 agataaaatc ggtaatttca aacctgaatg accggattta cgatattcca ttttttcata
    67801 acgtgcacta tctgcaatat acaatttttc tcctcctcat caaatctttt taaaacgctt
    67861 tcttttttct atttttagtt tactacattt taaagaaaag gagttaatca aaatgatttt
    67921 ttcgtatatt tttttgccat ttgtttttta ctcgttgata gatcagaaat tgattacgtg
    67981 cttgctggat ttttttataa cgtttcggtt gttttttgta aaattgctga tgatagtttt
    68041 ctgctggcca aaacgtaccg gctggttcga tttttgtgac gattggttgg tggaaagttc
    68101 ctgaactgtc tagtttctgt tttgattgtt ccgctaattc tttttgtcgt tcgacttgat
    68161 aaaaaatcac tggacgatat tgcttccctc tatcttgaaa ttgtccaaat gtatctgtgg
    68221 gatcagtaat ttgccaatag attgtcagca actcgctata acttatttct cttgtgtcat
    68281 aaatgatctc cacagcttcc acatgacctg tgtaaccacc acttacttga tcataatttg
    68341 gtttttcaac atgtccgccc gtgtatcctg acaaaacaga taggatccct tttttcgatt
    68401 caaacggctc gaccatacac cagaaacatc cccctgcaaa tactgcacgt tcttgatagt
    68461 cagcatcttg catggcatgc ttggaaaagt catatttttg ctccttatca aatccttccg
    68521 taatcaataa ataaaagtcc attacatcag gcgtcagatt cattcggata gctaatggac
    68581 gtagtgttgc ttctagtttt gataactgtt ctttaacgga tatattttct cttatgtcat
    68641 tctttgtctg aatcaatact tttcgttccc aatctcgtgt attcggattc aaaatcagat
    68701 tataaagtgt ttgaatgaat atttgttcct catccataga ttctcctcct ttccgaagaa
    68761 gacaagctag tgactttagc tgtaatttat cataaagtga ggaaataagc gattacttca
    68821 tccccctaca gatttttcgt ataaaaaaga gagaatcctt atcgacagat tctccttttt
    68881 aatcaattta ttttataaat gaaaagttaa tttacctttt atggaaaact attttaaaaa
    68941 ataaacataa atatgcagtt aatgtaacaa gataagaaat aatagtcgtt actgtataac
    69001 ttgtgcttac tttaatatca gcaactcctt tgactccgct tatttgtacc aatccattag
    69061 cattcacctg ctcctttaca ggttgatgat ttatttctac attcgttcct aaataagcat
    69121 atacaggaag tgtgattact tggctcttct cattagttac tctatatatg atagaactac
    69181 cagttgtttt ataaggaagg tttacccatt gactgccaat ctttgcttcg tgttttatta
    69241 tatgctcctt attctccaat gaagcgatag gagaataatc atataaaaaa taatccattc
    69301 ctttagtggt atacgcctct actgtttctt tgtttacaac aatatgtccc ggctgagaaa
    69361 ttattttttg attgaaattt tttttactag aaacagttaa agtgattaat aacaaaacag
    69421 tcataaataa ataaaatagt tctcttgtct tattgtatct tctatttttg aaaaatatca
    69481 tcgttaatat cagagaagag aatattattt gcaagcttaa gatccgccaa ggaaattgta
    69541 acaatttggc tggcgtattt tgcagaagat accatggcac caaactagtt gtagctagcc
    69601 aagtcaaaca cgtaccataa taaatctttt tttctatgtt tgttaatttt ttaaataaaa
    69661 tcaggggaat acataaagat aacagaccaa ctagtcccat tgagtaagta gttaagtcgt
    69721 tgttcatagc ttcagtcaga ctatcgaaca cattagatgc atgagcatat aaatttgtca
    69781 cgctaggttt gttgattttt tggtagagca tttgttcaaa catcggatac caaaaagcac
    69841 tcgtcagaac caatgtcatt cctgccgcac taaataattt tttaaatata gcaataaaat
    69901 ctgcattctt gaaaccttta gaaaccaaac gaataaaaat aaaaatgata attaatgctg
    69961 tactcattaa tgttgatagt acatggctgt acactagcaa actcatactc aatgctagct
    70021 ttacagacgg atacttttct tcttttccag agatgatttg ataagcataa aataatacag
    70081 ggataacaag tgttattgcg atagcttcgg caattgcgga acgatagtag atgtctactg
    70141 tccgataagc agagaaaata tagagacaac taaataaaaa tgccgctttt ttttgtttta
    70201 aaaaccgctc gccataatga taagctacga gtgttgtaca caagttcaat aaccaaacgt
    70261 aaataatata gcttacaatc aaattttttg tcatccaata taacattaca gctggaaaaa
    70321 aagtcagaaa gggataaaaa taatttacac catatccagt ataattgaat gtctcataat
    70381 taatcggact ggtaaaaata gtcgataacc ccttgattcg agataagtga aaagaaagat
    70441 ctccagtggt ataaatactc tgtgtatttt tgaaaaagat gaaagaatat atactactaa
    70501 ctataattaa tccactcaat attactactt ttttatttat tttcattctt cttgtttatc
    70561 tcctttttag tcacatatcg agaatctttc aacttattag gtgcaaatcg tttaaaactt
    70621 gaatacacca ctaaacaata agtcagaata ctgatccata aagccatgag agcagttgga
    70681 gtatattttg ttgtaatttc tatcttattt tctccagcat ttacatccac attcactaag
    70741 ccatgttttt tataagagtc atttacgact ttaccgttta cttttacctg tgtacctaaa
    70801 taagcatata ctggtactgt cactttttgc tttttgttgc ttgccactgt gtaagtaatg
    70861 ttattatctg cagctttata cttccctttt tcccacgatt ctcctacttt tacttcatgt
    70921 ttttctaagt gaggcctttc ttttatcgct tctgttgggg cgtaatcgta taatcctccc
    70981 atatctgatg ttgtataacg tgggagtgtt tcttcattga taacaatatg accgtgtttt
    71041 gattggataa cttttgcgta attttctttt gctgctatag acgtgacgag tagaaacacc
    71101 acagtagcgc caatgattaa ccatgaatat tttttattca tagtcttttt cataaaaaca
    71161 agcgttatga tcattgaaga aaaaaagatt tggataccta agatacgcca aggaaactgg
    71221 agtaagctta tcggtgtttt ttgaaacaaa aaccatggaa ataaattagt tacgcatacc
    71281 catgtagaaa ctacgccaac atagatgatt ttttcttttt tctccatttc tttcagtatg
    71341 aataaaggaa aaatcaacgc aaccaatcct aataacccca tagtgtatgt tgtaatatcg
    71401 ttatttaacg cttgaatgat actatctgaa atagaaagtg cttctagctg caaatctgtt
    71461 ttgaagggac tgtgtatttc ttgataattc atctgctgca acataggata ccaaaaatag
    71521 ctagttaatg cacaagtcaa aaaaacagcc tttactaaat tcacgaccac aaattttata
    71581 tcgtttttta tttgcttgct taaaaacaaa cgaactataa tcatacaagc aatcaatgca
    71641 ctacacaata gtgtagttaa gacatgagta tacacaagta gacttatact gacagataaa
    71701 gcgatgactt ttttttctct attgaatatc aatccataag cataatataa aacagctggg
    71761 ataatcgtaa gagcgattgc ttctgcaatt gcggatcgat aatagatatc aactgttcga
    71821 taagttgcaa aagtatataa acaactaaat aaaaaagcag catctatctt tttaaaaaac
    71881 ttttctccat aataaaaact aatcagaatc gtacaaatat ttaataacca aatatataga
    71941 acataactta atattaagtt atttgaaatt ccataaaaaa cgactgcagg aattattgta
    72001 agaaaaggat aaaaatagtt cagtccatct ccataattgt taaatgttgt atagttgatt
    72061 ggcccttcaa aaatagaaga taaccctttg attcgtgaaa gatgaaaaga taaatcacct
    72121 gttgtataaa tttcttgagc atttctcata aaatttactg aatataaagt actaagtaag
    72181 ataagaacta ctaaaagaaa tgctttttta cttattttca tagatttgtt gttctccttt
    72241 tttaattcta ttttcagtat actagaattg tcttttttta ataagttttg cgttaaattt
    72301 ttcatagcac tagtatgata tactagaaaa gaatcaattt ttattacttt taaataggag
    72361 aaaaaaatga aaaaaaacaa aaaccaaata atagatatca tttttatgtt atttctaagt
    72421 tttgcttacg tcatccctct attcatgtct aagggaatct atcatagtgt caatcaggat
    72481 acttactttc atttatctcg aatcatagga ctagataatg tatggagttc tccagtaaac
    72541 ttcaataact tcgatcatca tggaacaatg atgaatattt tttatccatg gcttactctt
    72601 tatccagctt ttttatttta taaaatgatg ggaaatcttg ttttaagtta taatatctac
    72661 tattttttta tcactttttt aacaatggtc gtttcttatt tttctatgaa acaaataaaa
    72721 aacaatcgtt atatttcgct gttattttcg attatttata cattttccgc ctatcgtgcc
    72781 attgacatat tccatcgtgc ttcattggga gaagctgtcg ctttaacatt tttgcctttg
    72841 atactaatgg ggtgttacga aatttatatt agagattacc aaaaatggta ctggttgtct
    72901 attggcatga cgctagtagt gtatactcat ttactttctg tcgctatggt ttctgtattt
    72961 attggaggaa cgttgtttct ttctttttat ttttgggacc aaaaaatcgc tagactctta
    73021 agtcttttaa aagctaccgt cctcaccttt tttttaagtg cgggattctt aattcctttt
    73081 attcaacaat ctagagcaca aaaattgaaa gtcccactgg gaaaagaatt aagtggtatg
    73141 gctcctagtg atatgctgac acatatttta aataataact ataacaacta tactattgga
    73201 ttattcttat ttctcggatt gattggtgct tttattttta taaaaaaatt aactgctgat
    73261 gatttattta ttttctttct tggtgtattt gttctttttt gttcaaccaa tttatttcct
    73321 tggcaacttt ttaaccatac accagttaag agtctacaat ttgtctggcg gttaaatggt
    73381 ttttcttctt tatttatagc ctatacgatg agtatagtga tctattatat tttttctact
    73441 aaaaataatt catggaaaat aatggtcttt acgtttcttg cactaatatt gcatctctca
    73501 ggagtaagca attctatcca tgctaacaaa gattcattaa cacttatagc acctgaagac
    73561 gctgttgcta tagctgaaaa ttacaatcat actgattatg caaacaaaga gtcaatctat
    73621 catcctgata tgattaataa tgatcaatat ttactaggta accaggaaat aaatccaaca
    73681 actcatttta tgagtaacaa acttacaatt gaattagata attcgaataa taaaaatact
    73741 gtcttgacga cacctattta tcgttataaa gggcaagtgg cttctattaa tggcaaacta
    73801 gtacaaacaa aattatctaa gtttggcaca actgaattaa ctattcctcc aggtataaat
    73861 aaagtcgtta ttacttatca gtacacaaaa ctagcaattg cttcaagata tttatctatc
    73921 gttactttga ttttattttt actttatcga tttacttttt ctaaacaaaa acaaccgaga
    73981 agagaagtac aacattctca ctagattttt atttaagaaa gactttttag ctgtacacat
    74041 aaacaggcta aaaagtcttt ttttatattt ctttaatttg cttcatacat tatttaacat
    74101 cgatatttat actgtgaaac atgcgtttca tccattatca aagtattttt atcgataata
    74161 aacttcatcc aaaaacagac cttgtgcagg gacggtttct cctgcatgtt gccgcacttt
    74221 gctttcaaat atttcatcga ttgtttctat cggcatggca cctgtgccga tttctagtaa
    74281 tgtccccatg ataatccgca ccattttata taagaatccg tctccgacaa aagtaaaatg
    74341 aagcatttct ccctttcgtt cgatagataa ttcatcaatc gtacgcactg tggatttttt
    74401 tgattttttc aatgcagaaa aaccaatgaa atcatgtgta cccactaatt tttcgcaagc
    74461 tgcattcagc ttgtccatat ccaactcttg agggaaataa aagctgtggt ttcgctcaaa
    74521 tgctgaagga atcacgttgt tccaaacata atagctgtat tttttgccca ccgcattata
    74581 tcttgaatgg aagcgctcag gcatttcttc gattcttttg atgacaatat cccgcggaag
    74641 gtaacgattc ataaagtcga tcatttctgc cagtccaatt tctgtgtttg ttttaaaatt
    74701 tgctacttgt cctcgcgcat gggtgccagc atctgttctt cctgatccaa taatttctat
    74761 ctcttcattt gtcatctgat gcaatacttt ttcgattttt ccttgaatgg ttttatcgtc
    74821 atctccaagt ctttgccatc ctgaatatct ttttccgtca tattcgattg tcaacttgat
    74881 gtttctcata ttttagtggc tcctctgtct tccatttggt cttctctcta cctttttaag
    74941 attgtagcac aaaatatagc tccttgcttc tctaagacag aagctataca agaaaactgt
    75001 ggaaatatat atttcagaaa aacaaaaaac agccaccgga tgaagtatcc gctgactgtt
    75061 caatttgacg gctcaagttt tactgtttga gatgcaagat actcaacctg gcaatgggca
    75121 cataattaaa gaaaaaactg attctttggc aaaaacctat tttattcagt aatggccaag
    75181 aatttataga aggaattctt gccagtccat aaatcgccgc aaaaagcaaa ctgatcacag
    75241 acagtccaag gtataagtgg cttacgtcca tttgcccatt ttttcgatag atcaagaaaa
    75301 gaagaactgg aaacaataaa aaaccactgt aacctaaacc tgatccgata ttatggaccc
    75361 atgtcgagac attccacgaa gattcatggg tattgatact gaacaaacct gtaaagatac
    75421 aatcacctat accatagaac gcgatggctg ctgtaactag ggtagctaac acacgagatg
    75481 tactaacaaa tgcagcatac aatgcaggca atgacaggat gaaaaagatc cctgaaacca
    75541 cagaccaaat aagaaacgct tttcttacag gactggaaac ctctccaaat acactgatca
    75601 cgctcttcat ttgatctaat tcaggataat acaatcctaa tacgtacgga gtcaaaaaat
    75661 cactcagcac gcctaataat aaaaagaaaa aaccaaattt acgtaaaaaa tccatgatac
    75721 gcctccaata acaattacct atgcttttaa ttgttattat aaacgacttt atcttgattt
    75781 aacagaaacc tctatttaca cgaacatcta tataactcat taaaaactcg aatcatttta
    75841 aaaatactaa atatctaacg ttcaacgttc acttaatcca tctaaacaat ctttcaattt
    75901 gattttttcc ccacttcttt tacgcgcgcc tagctctcct attgcagtaa acaacagatc
    75961 acttgacgaa tttaaggcag tttccattga atcctggata acaccgataa taaatccaat
    76021 accgacgatt tgcatcgcga catcattaga gataccaaac aaactacatg ctaatggaat
    76081 caataacagt gaaccgcccg caacacctga agctccacaa gcagctaaag cagacaaaat
    76141 acataaaagg actttcaaaa caaaaggtgc ttcaatccct aaagaatgta cagcagtcaa
    76201 cgtcatcaag gaaatggtaa tcgcggcacc gcccatattg atcgtagcac ctaacgggat
    76261 cgatatggaa taagactctt ttgtcaattt caatctttca gccaacatca tgtttatcgg
    76321 gatatttgcg gcagaacttc gggtaaagaa agcgggaagt gcgctttctt tcaaacaaaa
    76381 gaaaatcaat ggatagggat tttctttaat aaacaaaaac gtgaggaatg ggtacacaac
    76441 taatgcgaca aacagcatcg taccgatcaa aacaagtaaa agctgcagat actctgccaa
    76501 tccagcgatt ccagtcgtag caattgattg ataaaccaat cctaaaatcc ctatcggagc
    76561 aaatgcaata atcacttgta cgacttgtga caacgctata gatatctgat ccaccaactc
    76621 ttttgtcgat tctgaacgtg ctcgaaaagc tagtcccaac aatacagccc agaataagac
    76681 gccaaggtaa ttcgcatcaa taagagattg aacaggattt tgtgtgacat tcgtcaacat
    76741 agtagataaa acactttcca aactttgcgg tgctttttca gatactgttt cttgtaaagt
    76801 aataggaaca gtaaaaattg aacttacaat aactgctaca agtgcagaaa gcaatgttcc
    76861 cactaaatat aagatcaaaa taggcttcac gaatgtctta ttcccaattt tatgcttggc
    76921 aattgacgcc atcgtcaaga aaaaaaccag aataggtgcg attgccttca agcaactgac
    76981 aaacaacgtt cccaatattg aaataaatgt ccatgtgggg aaaaagatac caaacaatat
    77041 tccgactaaa ataccaacaa caatacgttg aactaacgac atatccaaaa acttctctaa
    77101 caaatgacca ccctttcaca attttaacaa tcattgtatc attttatgca aagaaactaa
    77161 aggtataatt ttctaaaaat tctaacaata caaacaacca tttcttgcta attttgtact
    77221 ttttctgtct acacgttgtt ttttgagata ttctaagtta ggatgttatt aatgggcaac
    77281 ttatcaggaa acgttttctt atgtttttgt ccaatttttt tgtgaggtgt ttattcttga
    77341 aaaaaataaa gaaggagagt tatggcttcc tgacaatctt tgtcgcttgt ataatgatgg
    77401 ttttacagcg tatattgatt tcagcagtca ctattcctac atatgcaaca aaccctggtc
    77461 gattctacat ctatacattt ttacaaactt ccgttgttat tggtgcaaat ttgatcccac
    77521 tttacatggg ttaccaatat caaaaaatcg cccgtttaaa agttttacat tatttaagtc
    77581 gttttgcctt tatattccta atagccacac tagtttgcaa tatctttttt tacgtacaag
    77641 ctggcagctt gaatattcgt gattattggc ttatcctaac acctatcagt cagaattact
    77701 ttacttatgc tgtttcttgt actttgcttt tttgtagtat tccttataca gttggatttt
    77761 taaataaatt atcgaaaaca acaattaaat atctactatt atttttaaca attggattag
    77821 ttggctgctc tactctattt aataaagacc cgtttgcatt acagaatgga cagagtattt
    77881 tatggatttt ttatttattt ttaatgggtt atggtttgaa agaatggaac tggaaagaaa
    77941 aggtccgcta taagcttcct caagtattta tcagcttgat tgttttattc ggtttgatta
    78001 ttttgatgac acaaatttca ctcatcatca gaggaaatac agatacagcg ctgaggtttt
    78061 ctaaaccata ttctctcttc gcaatgtatt atactttctc ttcgttttta tttttagaaa
    78121 tgctatctga taaattaggt ataaaaggaa gaggaaattt tcttgctgta tttttgattg
    78181 tcactcaagt tatatgtaat ttccctttag tttcttatcg cgtatctaca tttatgaaac
    78241 gtgaatttcc aaactcaggg aaggcatggc tgataaatat cgggcagttt tttatttgat
    78301 atttattagc agtgtccctc ctagttatct ttttatttta tatacaaaaa atccgtttca
    78361 ttcatcgttt catcgaatac ttcgcttttg attcgattga acaattaact cagcgttgga
    78421 aaaaaagaat tcattggctt tttgttcata aaagttattt tttagttgca gcattttttt
    78481 actgctttac cttcgtgcag attttcgttt tagaaccaaa agaaggttgg aaagaaacta
    78541 tccaggttgc cttaaaaata tttactcagc gacaagcacc catgatcctg acaatcatca
    78601 ttattatggg atttttcttc ttactattgt tgatttccaa tcgtttttgg tacgccttga
    78661 ctgctacctt gatcattaac ttactgctca ctatttcgac agtaatcaaa atggaaatgc
    78721 gggaagagcc tgtctttcct tcagatttaa aaatgctgac cggattgtca gaactattat
    78781 cgatggttag cccagtctta ttaatcgtgg gcggattgat ccttctccta ttattgatta
    78841 ctagtattat tgtacaacgt agactacaaa aacagtattc cttgaagatt cattggaaac
    78901 gtcgatttat cagtatcgct gtattattag gatgtttttc gggtgtcttt ttcattaatc
    78961 acaaaaattc gccttctttc ttgctgttta atttatttaa agtaaacaag actttcttta
    79021 accagcaagg cgccgtaaaa gataacggac cagtcattca attcctaaac aatatcgaca
    79081 ttaaaatcat ggaagaacct gctggctatt cgaaagaaaa aattaatgga ataatgaaaa
    79141 agtacaacac aaaggcagat gaaatcaacc aaacaagaag tgactggtta aacaatcaga
    79201 cgatcgttct caatttaagt gaaagcttca gtgatcctgc acgtgtccct aatctgacag
    79261 tacctacaga ccctattcct tacatcaaaa gcattataac agaaggaaca ggaggattga
    79321 tgttatccgt cggttatggt ggcggaacag ctaacatgga atgggaagga ctgacaggct
    79381 tgagtatcag taacctgtct gcttcgttag ttacacctta tacccagcta gtggagcgtc
    79441 agaaaataag tccaaatatt accgatctat tcgatgaaaa aatagccatc catcctttta
    79501 cagctgctct ttatcgcaga aaacaagtgt ttgataaatt tggttttgac aggttctatt
    79561 atttagacag tccggataaa ttaacttata cggataaaat agaacaaagc ccacgcatat
    79621 cggatgattc agcatacaaa gaaaccttga aagctttaca aagcaatacg gaaacgtcac
    79681 aattcatcca attatccact atgcaaaacc atatgcctta tgatgattac tatagtgaat
    79741 tgaactacac tgctgaagga actgctgtca ttcctaatag aagaaaagaa ttacaaactt
    79801 atatgcaagg ccttcactat accgatacag cagtaaaaaa attcatccaa gaaatcgaca
    79861 aaatcgataa accgattacc tttgtttttt atggtgacca tttacccgca atctatagtg
    79921 ggaatgattc gaaaaaatat ggattagaac agcaccagac agattatttc atttataaca
    79981 acaagtatag tcgtgaacaa gcagctacgt taaataaaga agttgttgct cctaataact
    80041 ttggtgctat ggctcttgag catgcgaata tcaaagtaac tccttactat gcgttgttga
    80101 cagaagtatc taatcatcta cctgcattaa caattgatcc tacagaaagc ttgtctaatc
    80161 gttttaatgg aaaacaggtc tttgttacag aggatagtca aattatccaa gaaaaaaatt
    80221 taactgagaa acaaaaagaa attctagaag attatcgtct gatacaatat gatcttgttg
    80281 caggtaacca gtacagtgca acatgggcga cacaaaaaat aaaagactaa caaataaact
    80341 gagcaagggg ttgtgataaa agacatgtca caaccccttg tttatctcat taatttgctt
    80401 gtccataaac gatttgatta aagccatttt tcaattcatt attcgctgtc tcgttgaagt
    80461 aaagataatt atcgggattc ccctgatttt gttgtgtcac caatactttt ggctgatttt
    80521 gctgaaaccc gaatagatag acgtgctttt tcagatatgg ttctgtctct gcatcggaat
    80581 agacggctgt gatttgatag tctgcttgac ctgtcccact ttctgaccat tctgcagaaa
    80641 caggcactcc accaatagcc atcttccact ccccgttcaa aatagcttga gggactttaa
    80701 gaccatataa gtccacactc atttgattcg tataactttt gtattgttgt cccattgttt
    80761 ggccccattt ggtcataaat gcctctaatt gtgcggattt gtctgcattc cacagtgttt
    80821 gttcaacttg ttcgctgctt tctgaagagg aagatgaagc ctctacagaa gaacttgaag
    80881 tagatgtttg agttgtttca gtcgttttgg aagactctga cgaagtagag gtatctgctg
    80941 tttctgtact tgctgcagtt gacttgctct ctgtgctagt tgttgtagta gttgcaactg
    81001 ttgctttcgc tgtttctttt gttgattctt ttttcgattc attcccgttg gtacaaccag
    81061 ctaacaaaaa agctagacca attgttacta aaatcctttt tttcaattaa aaaactcctt
    81121 tcgattactc ccacttcttt attctatcaa ataagcaatg tgattttatt gatttttcgt
    81181 attttctcat caatctttca aatcttctaa aaactgagaa gcacccgctg atgttgtctg
    81241 taatacatca gcgggtgctt cttttatttt aatcttcgtg attttccaat ttacctcacg
    81301 ctttatattt gtatttgaag aaacatagac ttttagtcat catcgtcttc ctcatccaaa
    81361 tcttgtcgga cagtttttcc ttggagtctt tgtacttggt agatgatttc tttattgaaa
    81421 cttaaaagtt caacaagcaa ttgttgcaag ccatattttt cttctttttc agctaatttg
    81481 attgcccgtg tgataaagac attttggaaa tcaaaatctt ttgcaaagtt ttccaacatt
    81541 gttgaggctg gtaaatattt gttttgacca tgttcgtaaa tttttccata acgcatgact
    81601 tcttctaaag tagacggaac gatttcattt tcaatcacta actcatgaga gattttattg
    81661 aaccatctcg cttcttgatc agcatattct gaaaaaaggg tacttaattc ttgcgaagat
    81721 gtacccaaaa catacaattg tgcttggatc aatttcattt cttctaattt aaaatttgct
    81781 aatacatgcc ccaccatggc gccagcagtt ggtttatgat ggtcgatatc tgcttgtttt
    81841 gtttcttgaa caaatttttc ttccactata ttcattgatt tacgcctctt ttacttttgc
    81901 tgattggact tcataaccta atttttccac aactgcagct aattgttcag ggtggacttc
    81961 atctggatca aagtttgctt tgattttgct cgcattaaac aaaactttcg tattgcttac
    82021 cccaggctgt tgagaaaccg catgttcgat tttttgcata cagcttggac aagctagtgt
    82081 atctaattgc ataataattt ttttcatcgt agttcttcct tcctttttct tgatagtctt
    82141 attctagatt acagcttcca taaaaaaatt gattcttatc aagttttacg atttttcttc
    82201 tttgctttcg tgtccaaaat acaataaacg catcgcgtta aagataacga tcaagatact
    82261 agcttcgtga acgaacatcc cactagccat gtaaataaac ccggcaaata agccgttgag
    82321 taaaaataag acgacaccaa tggcaatcac tacattttca cgggtattta aaacagtttt
    82381 cttagccaag cggtaggcat gaaccaacga ttcaaaatta gattgcatca agacaacgtc
    82441 actcgtttcg atagcaacat ccgttcctga tcccatagca atcccgatat cagccgcagc
    82501 caaagaggga gaatcattga ttccatctcc aataaaagca acgcgcaatc cttgttcttg
    82561 gtattttttc acaaattgaa ccttttcgtc tggtaataat tcagcatgga cttcatcaat
    82621 gcctaacata tcagctacat aatcagcagt catctgatta tcacctgtca gcatcactaa
    82681 ctgttttgct cctgcttgtt tcaacttagc taattgttca gcgacttctg gtcgaatcac
    82741 atctgacaca cctatgattt gagtgatttt tttgtcgatt gctacaataa tgaccgagct
    82801 gcctgtttgt tgtaattgat gtaaatcttg tacttgcttt ggtgtgagag aaagtttgtg
    82861 agcggcaatc aatttggtgt tgcccgcaca aactgtatga ccatcaatct cagctgtgat
    82921 tccttgtccc ttgattgtct gattatcttt gactgctaat tgttgaaaat caatgtcttt
    82981 ctcctgagca taatggatca cggcgcgccc cagaggatga tcggataacg tttctaatcg
    83041 agccactaaa gctaagctgt ccccttcatc ctctgtatag ttcttgatag tggatacagc
    83101 agtagttcct ttggtcaatg taccagtttt atcaaagaca aaagtatcga ttttggaaaa
    83161 agtatccatt gcttcaccgc ctttgactaa gatgccccgt ttcgctccat taccgattcc
    83221 tgctacattc gaaacaggcg caccaatgac taaagcgcca ggacaaccaa gaaccaaaac
    83281 agtgattgct aaacgaaaat cacgactaat cagaccaaca accaacgcaa taagtaaaac
    83341 ggttggagta taatactgag aaaatcgatc aatgaatttt tccgcatggg atttggtatc
    83401 ttgtgcttct tctactaatt cgataatttt ggcaaaggtc gtatcatctc cgaccttagt
    83461 tgcttcaatc gtcacgtagc catctgctaa aatcgttcca gagaagactt catctccact
    83521 gattttgacg acttgtcgtg attcaccagt cactgcggct tcatccgcat accctttccc
    83581 gtcaatgact cgtccgtcca caggaatagc tccaccagtt tttactaaga cgatatcgcc
    83641 ttcttccacg tcatcgattt ccacttcttc agtcgttcca tcttcttgga caacactagc
    83701 agtagatggg gccatctcgg tcaaagcctt gattgactcc cgcgttttgg tcaatgtttt
    83761 ttgttccaga tagctaccaa acaaaaacaa gaatgtaacg atagctgact ccttgtattc
    83821 tccgatcata aaggcaccaa gcacagcaat agtaacgagt aaatcaattg agataacttt
    83881 gactttcact gcctgatacg catggatcat aataggtatg accgcaataa tagaagctat
    83941 ggctagagca atgttataga acaagacaaa accagtaaat tttccgataa aaccaaaaat
    84001 aatcaacaca gctgataagg cggtgatatg atttcgatgc tcttggatcc acttttgtaa
    84061 tttcatcttt ctccctcttt tcctttcatt cttacgagac taagtataga tacttatcca
    84121 tcataaaaaa ttgaccacta tcaagtttag tcaaaaaggc ggttttcttt tggtagaata
    84181 cactacaaaa aatagttagc aagaagactc actaaaagtg atcttgccaa ctattgtcgt
    84241 ttctatcgaa attttcattg aatgattttc ctgtacagca tacatcggag atctatttct
    84301 ttatctaaga tttttatctt gctaattttt tcctttagtc tttcaaatcc acgtcgttca
    84361 tagaattgat acgtacagcc actatctgta aaaaggaata tttcttttcc tttctctctt
    84421 ttttcaaatt ctgttagcaa cttggtaccg atgccttttg ttttcaaatc aggatttgca
    84481 gctaaaaaaa tgatctctcc atctggcgaa tgcttcttta aatatgctgc aagcatttgt
    84541 tcatttgctg tctcgtaagc tcctttactt tctttagcaa atatattttg taaaaaatcg
    84601 aatatcttaa catatatttg ctgtccaaaa gaacgatgtt tctttttttc tccttttatc
    84661 tccgccagca aaacacctgc caactcgtcc tctgcataca aagcaagaat ttgagttgct
    84721 cgattcaact ccatatacaa aaaatatctt ccgtaaagat tcagcagaag ttcattttcc
    84781 atatatctct caaagttcat tccttcaaca gcaaaataga tagctttttt gtaatctttc
    84841 ttttctaagg gtttaaaagt tagtttcatc atttcgcctc ctcaacttca atgattgtgt
    84901 tatacttaca ttataataga cacagtgtct actatctata atcaatcatc tataagtttg
    84961 tcgattaagc gacagctttg ctttgatatg tcggataagg agataggtaa atgaaaaaga
    85021 cagatagacg cgtcaaaaaa acagaaaaag ctttggctga aacactatcc acattattag
    85081 taaataaaaa gattcaagca atcactatac gcgaactaac agaaacggca gatgtacatc
    85141 gttcgacttt ttatacacat tataaggata tctatgatct atatgatcaa ttagaaagca
    85201 acttttttcg ggatctaaat gaaattctct cttatgatcc agcccatagc tatgaggaac
    85261 tatacactcg cttgattgac tatgtttacg caaatacttt tttatatcgc ttatttatcc
    85321 tcaaccatgg tgatatttcg tttcagaaga aagttaaaca agttattgaa gaaaactatc
    85381 tgaacatatg gctatatgaa gacaagaaaa cgattatcac agaagaaatg agattcttta
    85441 ctacgtacca cgtccaaggt tgtctatccg ttctcaatcg gtgggtagaa agcaattaca
    85501 agtactccaa aaaagaatta ttagaactct tacagaaact gaatgatcac atagagaaaa
    85561 tcatgccttg aataaaaaca tactaagctt agttaagaag aaccgtaacc ttctcgtata
    85621 acaactgaat cttatttagc caaattgttt actgaagatt ttttgagtaa aatttctata
    85681 attcctactt attgaaaagt agtttgtagt gctgcttctc cctctctaag tgatatatct
    85741 tgttgtacca atcaaattca cctcatcatt aataaattac ttttaggcta atttattaca
    85801 acttataggt tgaagctgta accaatttat agtcatatta tatttttatc atccagttta
    85861 ttgaattctt ctcgtttacc tcaattcatt ttcaagggga atcagctcat catctaacat
    85921 tttacgccat gctcgcttca tttcttgtgg gtatttaaat ggtttcgctt ctggatgttt
    85981 ggctttatat ccttcgtaat ccttactgtt gataattgct ttcatattag gaattggatc
    86041 ttcacctggc atatattgca gattagaagc atgtaatttt ttctcagaaa caaaccactc
    86101 attggaaaag acatgaatca aatcatcact tacttgtgta aaatagcgac gtttaatgac
    86161 aaagatatct tcattgctat ctagtgtacc ttcatttctt tgtgcttaaa ttcaaagata
    86221 attttctcaa taaagatttt tgattccagt aagattcctt tactcctttt acaacatagc
    86281 gttgtccttt gttttgaaac atcttcttcc cctctttgcc attaaaaata gcccaattct
    86341 ctataaagaa gattaagctt cattattata atataaagtt taaatttggt ttattactta
    86401 ctttttctta ttctctagta agctaatatt gaaaaaaatg taatcagatc attattttta
    86461 tatgttgtaa cttcctgata agaaaactaa ttaaaagaaa gaggtaacta cataaactaa
    86521 ttaaaaaagc taacttcata gttggaggat aaatggaaga aacttcaatg attgtttctc
    86581 cttgtgttcc ttcaatcgac actagtccta ttatcaatct ctcttttttg tccatttact
    86641 tttgcgactg ttccccaata atcaaataca ggaagcgtta ttttttgtcc tttagattca
    86701 tgtactgtat agactatttt cgaatccatc acttgataag gcatagtatg ccattccttg
    86761 ccaattctta cttcatgatt ctgcaaatgt tctttgtatt gtaatgcttc cgtgggtgca
    86821 tagtcataaa gtcctccggg aaggagtgtc gtgtaacgaa taagattttc ttgattcatt
    86881 accaagtgat ctctctgagt gacaacttta tgttcatagt ttgcttttgt tgcagcagat
    86941 acagccaaca gtaataaaac aattgccgtt atattaacta cttttctttg cttagctgat
    87001 gtgctcttca tgaaaacgaa cgataaaatc aacgagccaa acagtacttg aaagcctaag
    87061 attcgccaag gaaattgaat catttgaatg ggtgtatttt gtaataaaga ccatggaaaa
    87121 aatttggtgg ataagagcca agagactacc gcactaaggt aaatcacttt ttctttgcga
    87181 ttaaatcctt taaaaaagaa gagtggacag atcaaagaaa taataccaat gattcctacc
    87241 gtaaaggttg tcaggtcatt attcatcgca ccaatcaaac tatccgagac atttaatgct
    87301 tggtgttgta aggtcgtttt atctggctca ttaatcgatt gaaccatgat ttgtcggaac
    87361 atcggatacc aaaagtatgc tgttaaaaga atcgtcattc ctactgcctt tgacaactcc
    87421 acaatcaacc ggatgctttc tttttttctt gtttcactta ctacaaagcc gcctaagact
    87481 agtaagccaa cggctattac actcataaaa gtggaaagaa catgtgtata aatgagtaag
    87541 ctcatactga ctgctaatgg aattgcacat ggctttttct cataaatcag tgcgtaagca
    87601 taatacataa ctatcggtat aaccgtaagt gcaatcgctt cagcaatagc agaacgatga
    87661 taaatatcaa tcgtacggta cccataaaat gtatataaac agctaaatat aaaagccgca
    87721 tcgattcttt ttaaaaattt tagtccataa tgaaacatca gcatgatagt acaaacattc
    87781 aatagccaaa catataagat ataactaacg attaaattat tacttatcca ataaaaaatg
    87841 actgccggaa aaaaggtcaa gtagggataa aaataattca ctccgctccc ataatgatta
    87901 aacgtcgtaa aattgattgg accagaaaaa attgaagata accctttgac tctagcaaaa
    87961 tggaaggcaa tatcccccga tgtagaaata gtatgaacat ttctaaaata attgattgaa
    88021 taaagtccag caataattgc taggagaatg gacaatatta tttttttctt tacttgcatt
    88081 acttaattat atactccttt catggattgc tttatctagt ataacttaaa ttttaaataa
    88141 atcgttattt atttttactc tcacttaaaa aagcactaaa attataagag agggaggtaa
    88201 tggtctgaac accaagaatt gttccaacat cgaattgctt aactaatgag ttcaaagctt
    88261 aattatgtgg ctcttatatt taccgagcgt ggattgcgat ctctttagcg aaacaaacat
    88321 ctttcataaa aaaaacagcc atatccgctt tcttggatat gactatttac tactacttta
    88381 ctatctttca ttcttcttgt ctcacttatg ataaggcgct gcgtatcatg agtgaatgat
    88441 attaggaaaa cttcataaaa taaatctttt atacatttcc acttctagat ggtcattcat
    88501 gttagaagaa tcaaactatt tttttactgt cgtaataata ttaaaacact agaacaaatc
    88561 aattaaattc cataaaatat aaataataga cctagcatca tctggtataa aatggatgct
    88621 aggtccgtta tatttactca aaattgattc tacgaataga tcttcttatc atttctattt
    88681 tagtagtcat gcgttgttgt tcttctaaag gtggtaatgg tataagtagt ttagataaat
    88741 ttgtaccatt tacatttggt tgcccagttc cgtaagaaat tttttctaat aacttccaat
    88801 ataatggcga gtctaagaag cagtctacgt attctgataa taatttctca actaacctaa
    88861 ttcgaatcaa gtaagaagca aaaacagatt cctcacttat ttccttaacc aagaaacttt
    88921 ttcccatagt tcctcctgtc cttgctatca aaatgtcatt ttcttccaat cttaaatcaa
    88981 ctagtttaga attactaata tcacagtaag gcacactgga ccaatttaca cgtccctctt
    89041 gtatatctgt gattctcaaa aacttaacat tgccttgttt tttcgccgaa tctgtatagc
    89101 catattgaat cttagtagaa atacttccta aataaaccca tagtcgaaca tcgctagatg
    89161 atttttcttg tcgttcttta tgaacaacag tgatcatctt gagttgtccg actatttttt
    89221 agttgcagca atctttttgg gacgcttatt tgcatgaagc aacgttgcta agtctagtaa
    89281 taaaacattg gctaacacag gattggcttt ctcgcgatac gctgcggcgc cactttcaaa
    89341 caatactgct agacgatctt ctacaacaac gatttgttcc aaatacggtg gaaatttgat
    89401 tcgttttagg atccggctag gagagtcccg ctcggacact aagagttccg aggtataagg
    89461 accataagaa cgactggcta agagataatg gccataaaaa gccacacctt gaatcttatc
    89521 agatgttttt ctgctcattg tcacggttgg ttggattttg tctttgcggc gaattgctgt
    89581 tttcatttca gctatcgtcg gcaatagata gaaagtcaac tgtccttcac cttttagtgt
    89641 gaagttgcca gataccagtt gatcattgtt ttgagtcaaa tacgatgctt gtggaatctc
    89701 tgagagatca gttgtcagcc aataagcgat tggctgtccc gtctcttcag atgtatctgc
    89761 taagatatcg atcaaacgta aagcggctac tcgtccagta gcagatcctg tgattgtcaa
    89821 ccataaaagt tcccgttttg tatcatatac taagccccct acatgtggcc gtttaggtaa
    89881 aatcagcagt ttgatcggct gtcctgtttc tctatccagt acatgaataa tagaatgatg
    89941 ctgatggtca tagcagtagg cagaaataaa cacaaaatct tgagaaaaag ccaacccttg
    90001 cggcgtcatc cattgacaaa cctcattttc gccggtttcc ggcaccacca ttcttgtttc
    90061 tgttaatcca ggaatgttat attgttttgc ttcagccttt tcccaagctg cttgaacaat
    90121 aggataccgt tttaactttt tttcaatatc ctgatgtatc cgttgctgct tccaatgctt
    90181 aaactttagg acatagtaaa caagaaggcc aatgaccagc aacactaaaa tcattattgc
    90241 tacccgctgt gtcatttttt tgcctccttg ttctattcga tcaatcgtcc atataccgct
    90301 attaattacc gtaactacat gtttctttta ctagcgtttc tttataggat ctttcataat
    90361 ctcatttata gtaacattaa gaatccctta ttctactata gtcacttcca catcaatatt
    90421 gcctcttgtt gctttagagt atgggcaaaa tgcgtgggct tcttctgcga ttttctctgc
    90481 ttggtccttc tcaagtcctg gcaaagaaac ttctaaaacg accccgattt ggtagccccc
    90541 atcttctaat tgaaataaag atacccgtgc tttgacttca gaatcagact caagattatt
    90601 ttcttccagc atgtgttgaa ccgcgccgtt gaagcaagat gcgtatccag ccgcaaaaag
    90661 ttgttccgga tttgtccctt cactatggat tcctgggcgg tcgatcttca tgtgcatact
    90721 tccgtttggt gcctcaactt cgccatcacg gcctccacga ttgatcattg ctgtttcgta
    90781 catttttttc attgagatca tcctttcttt cgtcgttcta tattaagtgt aacgagtatt
    90841 ttttaatctg acaaaggtaa tgcttgcatt cggcgacatc agttagacaa catcgatgat
    90901 cagtgataga agcagtttat ctatacaaaa aaagaagggt tctatgatat ttggtgtcct
    90961 tactcgcctt gagtaacaac accaaatatc atagaaccac ttaatcagct tcacactgct
    91021 ttttctactt tcgtagttga aaaaaactta aacaaatcat cggcaaatag ttaaagaaaa
    91081 aacttaatcg ttgaaagaag cccaattgct taaagaaggc aaactgacta atcgacggaa
    91141 tccgtgctaa tccgtaaaga cctgcaatta gtaaactaat aactgttaaa acaagataaa
    91201 aatggctaag cgtaccttga ccactttgac gataaagcag aacaagtaac aatgggaaaa
    91261 gtagaaagcc tgcatagcct agcccagaac ctatattatg gatccaagtc gatagattac
    91321 aagagctttc attcgtattg atactgaata agccagtaaa aatacaatca cctatcccat
    91381 ataaaccaac tgttgcaaca acaagtattg caagtgtttt tgatgttcca acaaataggt
    91441 gatacaacgc aggcaacgaa agaacaaaaa aaagtccaga tacaactgac cagaccaaga
    91501 aagcacgtct gactggacta tctacgtcac caaacacact aattacggta gtcatttgat
    91561 tcagtttggg ataaaaaagg ccaaggatat acggtgtcga taaatcactc atcacaccta
    91621 ataataaaaa ataaaaacca tatttttgca aaaaaatcat tttcttcccc tactttttag
    91681 tgattattcg atacatccaa caatcaaatt ttttctcatt ttaacacata tttttattct
    91741 agttgtcttt tgaacaatca ataacataaa ggtgagtttc ttctacattt taaaaaatcg
    91801 aatggcactg aagagttcag ttgtcagcat caatctccta ctcaaaaagg caaaaaaacg
    91861 taagtcagat gttgttgaca tctcacttac gcatccattg attgaaaatt tcttagtaaa
    91921 atctactagc ctaaaaattt gaagacttct tctcgatcat acagtttaaa tactttctcg
    91981 cataaaaatt gagtaattag tgccgcaaaa acagggatga ctaaccaaca aagaacaaca
    92041 agaaagccat ttagccctgc atctaaagaa gccaacggac caacaagtcc gaccacgccg
    92101 aaaccagcag aagcaggcgt acctgaaata gaaaatagag cgacaggaat agctgtaatc
    92161 actgcactaa ctaaacaagg aataagaatc acaggttttc taaatagatt aggcatcatc
    92221 atcttcatag cgcctaaagc aatcgcaatt gtcacacctg acttattaac cttccaagag
    92281 ttcactacta aaacaaaagt ggttgcagct acgcccatcg cagccgcacc agcagaaaca
    92341 ccatctagtt ggattgccat tccgattgct actgttgaaa taggagagat aatcaaaaag
    92401 gcaaaggaac aaccaatcaa gatagacatt aacagcggtt gtaaggtagt gaagttatta
    92461 atcaatgatc caattgcaga agtaattgct gttacaaaag ggaataaata aaaaccaagt
    92521 aaacctgcac ctacacctac aacaatcggt gttaacacaa tcgacactga cccaaaacta
    92581 tttccaatca gtaaaatcag cccgacagca atcgatgctg tcagcatcgt attaataata
    92641 tcgcctgtac cagcaccaac gtaaacaccc gtttcaccca aatcaggtgt gaatttgact
    92701 acaccagaac caacaaaaga agctccggct acaaccatca ttctcatcgg atcaaatcca
    92761 aattgtaagg caatcaaacc accaatgatc aaaggtgttg ctagttgaaa aataactgct
    92821 aactgtgcga tcgttaccgc aaagggatac gcactaaaat attttaaaat ggcacctaat
    92881 actgcattag gaatcaatcc aataatgatt cctgttgctg taccagcgag gactttattc
    92941 agaaaaattt tcggtgatat tttttgtgaa gtttccattc aatctctcct ttattttacg
    93001 ttcaaactat cttcttttgc atggacaagc tcagtcaaga aatcacaata aggcgtcgca
    93061 acaccatatt tcttcccttt tctagaaatt gcgccattga tataatcgat ttctgttaat
    93121 cgatggttct taatcaagtc ttgatacatt gaaggataat gtaacccaat tgtttccggg
    93181 tcaaaacaag attcacaatg tgcaatgact tctgggacat ctagttcaat cttctctact
    93241 gctgctactt tggcaaactc attgacaatc gtcgccacca ttttatgagc agtcgatgtt
    93301 tttcctagct cagccatatt aacgtctaag attgtgcata gtccgttcat tgttccgttc
    93361 acacatgctt tgcgataaat agaatagtga atattgtcag aaaaatgagc atttaatcct
    93421 gattcagaca atttgtctgc taattttttt gcagctgctt cttttccatc acctaaattt
    93481 tgtaattcta ctgaaccact tccaaataat ttgacctgac caggaccttc aagacctgcc
    93541 gtccacatgg tatttccaat atagatattt tccattggta caaatttctc aataatatct
    93601 tcatgcccga taccatttaa tagacataag acctctgtat cttttttgat taatgattgg
    93661 atatcctgca gcattttttc cagctgcatc gctttggtaa atagaataat cagatcaact
    93721 tgatcttctt tttctacttc ggattgaaga acgattggta gttttgcttc tacctcttta
    93781 ccattaaaat ttgcttgtaa tccatgttct ttgatttgtt ggacatgttc tgcccagcca
    93841 tcgattaata atacttcgtt cccactttgg tgaagcatca acccaaagcg acttcccatt
    93901 gcgccagcgc ctgcaattgc tattttcatt gattcttcct cctttttctt tctattatac
    93961 tcatttgaga atcttttcac aacctcgttt tgttattctt ctcttattta tcatgtttat
    94021 atccaccatt caagttattt tatcagcaaa tcaataaaaa ctggaataga atcccaattt
    94081 catcgtaaga gaaactagac tagcaataag aaaatagaaa cattcataga aaaaatctca
    94141 ctcctctgat tataaaagag ggggcattaa cctcgtgtat actaaaacct tccattcatt
    94201 cgatggatga ttttcttttt gtgaaaaaat attcattgta gtacgatgtt gcgcaacaca
    94261 gacggatggt attgcttatt atttattaaa atcgaacaat acacttattt aaaaacttct
    94321 ttcccagatt ttcttccatt tttacagtta taactagtat tatcatgaaa aatagccttt
    94381 tattgtctat gggaataata atttaaaatt ggtacttttt tattaattag ttcatcgccc
    94441 aataatctct ccagtttctt agcatctcct gactcaaccg gaattatcca agcccagtct
    94501 aaataatttt ttaaaatctc atcatatttt gcaaatattt cattgccata ttccgtcctt
    94561 aaaattatat tttcagtgcc agacaagtga tttatattga gttgataaaa aagatctcca
    94621 tctaaaaaaa tatcattaga agaagatttc cccacagtaa tgaccattcc atcagctcta
    94681 tgtgttacat acaaataatt gtatgacctc atttcaccta atgaatcaat tatttcttgc
    94741 cctttagctc taatatattg atctctatta cctatctcac tcttatttaa caaaagtttt
    94801 ttcatgtcaa atttaatcgg ctgttttatt tttgtaaatt cgcttctact actaatccaa
    94861 tccgagatta atttttcaac atgtcccaga gtaaactctg atttattcgc taaatacttt
    94921 ttttgttgta ttaaagacat ctttccccga agctcaaaat tgagcctaca tcgattctct
    94981 atctcttcaa aaagcatctt atcttcatca ttcttatatt catcattcat aaaaccaatc
    95041 cagctcctcg attaagataa tattttaatc tcatacaaaa actagctctc atcaaaacaa
    95101 ccattgtttt gatttctcat gaaatttctt tatacttttc tccgattata aattcctaac
    95161 cccttgagaa aattacgcat atatttatct ccgcaaggct taaaatttcg ttggccttct
    95221 tttctcaaaa atgccccgat ttcgcctttt gatggataga tttctgcctc agcaaaaacg
    95281 tctaaaatat catcagtggt catagacaag gcgattttta atttttttag caacacattg
    95341 ttgatatatt tgatttcctt gcttttttgc ggcgtgtcta ctatcggctt atctttttgc
    95401 ggaccacgcg ccaagagaat caggccattt aggaatgcgt caaaaacttg gttgttgatg
    95461 gttttttcat acacattatt ttctgcatta tctttgttct tatcctgtac tttggtcagt
    95521 accgcctgag cttcttcttt agtcagtggt acaccgccta gctcaaaagc tttaatcaaa
    95581 tccgtatctt tcaaatcaat cgcataacgc aatcgcaata acctatcatt gttattcatt
    95641 tgttcctccg aagtatttag tctttacttt cttacgtact ttttgtcttg ctaagatttt
    95701 taggatcaat gataaaaacc gcatcactta tgataaggcg ctgggtatca tgagtgaatg
    95761 atattgctga atacaaatag tccgaccaca ttaagttgat cggacttcat aagcatttta
    95821 ttgactaata cttttttgct taagaattga ttctaacatc ttttgctaaa gttaagcatc
    95881 ctatagttcc tgcattatct gctaacttag gtgtgacaat gtattcttcg atatttggcg
    95941 tatcgacata gccgttaatt agttctaaaa aacgtctttg tactttcttt ttcagatgtt
    96001 cttgttgcat gacaccacca ccaaaaatta taatatctgg tgaaagcatc agggtcgtat
    96061 tacaggcaca ttgtgcaata taatccgcct cgatttccca gaacggatct tctatagaaa
    96121 gatcttgtcc cttggttccc gtacgcatct cgatagcggg tccagatgcc aacccttcga
    96181 gacaatcatg atgaaaagga caagtaccag agaaagtatc tcctgaatgt ctcttaacta
    96241 acgtatgccc catttctgga tggctaaacc cttcaacaaa aatcccatct tgtatagctc
    96301 ctccaccaat tccagttcca acagtgtaat aaacaacact ggataaacct tttccatgac
    96361 ctgaaacata ctccccgtaa caagcagcat tcacatcggt tgtccaggca attggaattt
    96421 cgaaatgctt attcaacgtt ccaacaaaat caaaattttt ccaggcagtt tttggagtag
    96481 aggtgatatg tccataggtc tttgattcac gatggatatc aatcggtcca aatgacccta
    96541 ctccaatgct ttctaattgt tctttatagt gattaaagaa atcaattacc aaactcattg
    96601 tttcattcgg tgtagttgta ggaaaactca ctctttctaa aacctttaaa tcatcattgc
    96661 caatggcaca aacaaattta gtcccacctg cttctattga gccatacatt tcttactcca
    96721 ccgtcctttt atctaggaat tcgttcttcc ctgtctaata gtttttcgaa aaactcatgt
    96781 ggttcctttt ggtaccaata ggcaacagaa gaaatatcgt cttgacgctc ataaagcttg
    96841 atgtcatcat ttcctagggt ttggaaagta acacgtaaat tttctttgaa actaatgggg
    96901 tcgggtaaat gccagcggta caacccatgc atcggtaaag catcatcgcc aaaaccatga
    96961 actggatttg gattaggttt tccagcacta aaataatccc gggtgtgatc tcttttagat
    97021 tgaaaaggat agcccataaa tagcgtatta aaattttgag catatgccct ttctccaaag
    97081 ttctttttat ggaaagccca agcgccgcca aaataatctt ctgctcctgt agatgttatt
    97141 gtagggaaat cttcatctcc atctatataa aacttaaatt ctccttcccc ccaccagtat
    97201 ctttccaatg cgcacaaagc aaagtaggtt cctacataat acccagtgcc tttgattcca
    97261 tcaataaggg tgtaatcttt tccttttgtc gtgatgggtt cacggttcca tttagcatga
    97321 aaatacatcg catcatccgg tacttcagtc aatgcataat taaatgtata aaaaatgtgc
    97381 ttaataaact caggatgttc attcgttaaa gtaatcttag ctttttttcg aaatggcatc
    97441 tcaaaatagc aattcatccc tgatgttgga ttcactacga ttggtaagga attcacttcg
    97501 catcttgtcc caaacccatt acaaaaaaaa tctcctaatg gactttctac agaaggagtg
    97561 gattcatcat cccaataaat acgcaaaatt aaatcacgta agacgaaact cccagcgatt
    97621 gttttgtctg tcactgtcat ccaaaaatga cgtaaaattc cagggccttc catttctgct
    97681 aaagtaattg tctctccagc atttatcgga acagaaggtg acccttttct cccaattcct
    97741 agatgactcg ctgtcattgc cgctttccct ttttcaccat ttttattttc tggtgaaata
    97801 gttctacttt cctctccctt gtggagccat atattattta aacaattcat tttattcgtc
    97861 ctctatcctt tcttcacttt tttgattatt catatagatg atttgttgtt gtgcctccgc
    97921 aataattgct ccaatatcta aattatcatt tcctaactgc aaagcgattg acattgccaa
    97981 gggaagattc attcctgcaa ttacgtgcac tctgtcagaa atataggtaa aaaacttttg
    98041 atttacactt cctccagcca tgtcagttaa aacaattatt tcgtctgtag tatccactaa
    98101 ctctttaatt tgttcatcta tatcatcaat ttcttcgaca tatgcacata agctactaat
    98161 ttgattatgc atacctgtta aaaactctag tgtctcttta aaaccctcag ctagtattga
    98221 atggctagca atgatcaatc ttctcataat tcaaatcctt ttcctttacg ctaatattcc
    98281 aaatgcagcc ataaccatcg caaaaacaat aacgagtaga atcaaaatgg tcatactgat
    98341 ttttttctta ctgagtagat aatatgctgc agcagtaata attactggaa ttaatgttgg
    98401 taaaattttg tccaacattg gttggatttt catactaact tcaccaaaag tgaatttaag
    98461 tggcgttgaa acactgataa ctgaagcaat taatgatcca actactgtta gccccaatac
    98521 tgaagcagct tcagttaaat aattaatctt ttcaccgtat tcagtaacaa tcttcattcc
    98581 ctgcttataa ccaaattcaa agcctctcat tcggaaaaag aagattacca tgattaaaat
    98641 gaaccataaa aagattccta caggattatt ttctaaagcc atataagccg caattgatcc
    98701 catgatggtt gggatcatcg tcatcaaaag agagtcccca ataccagcaa ttggacccat
    98761 cgtaccaact ttaaagtcat taatcgcatc ttttgcttct aatccttgct tttcctccat
    98821 tgcaacacaa gcacctaaaa taatggctgc cagccaaggc tgtgtattgt aatactgaaa
    98881 gtgattattt aaagcttcga tgtattcatc atcatctttg tataattttc ttaaaatagg
    98941 tgacaatgcg taagtcacag atgcaccttg ctgtgtttga taattaaagg tacaaactga
    99001 catcaaccaa cgatagttcg ctttcctaag atcctttttc gttacttgat atttttcgct
    99061 cattttattc atcctcccca ataaacccaa catcagaagc tcccgcagaa gcagtgattt
    99121 gtgcatcttt ttcagctcta tgatacacat taaatgctaa agcgaatcct atgatcgccg
    99181 ctccaataat aggtaccttc aaaaaagcgg ctaagaaaaa tccaacaagt aaataactaa
    99241 tgtatcgacc tgtaggcata aagcgtaaca acatcgcaat accaacagca ggcaacattt
    99301 ttccggctaa tgttagccca tccataaacc aattaggtaa aacatccaaa acgtaattaa
    99361 cagcagactg tcctaaagta acggcaataa atactggaat tgctgcagat aaaccaaaga
    99421 aaattggccc caaccacaaa acactattca ttttcttaaa ttctcgttta ttcgcatatt
    99481 cctgtgattt gcgaacaaca aaaccattta agattttgac aattacatct agttgcaccc
    99541 ctaacatacc tacaggtagt cctattgcta atccggcttc tactccaagc tggctaccaa
    99601 atgccgttgc aataattgcg cacgttccat agtccggtac agatgagcca ccaaaaccgg
    99661 caactcctaa tgacatcaat tgaactgtac cgccaactat caaacctgtt tttatatcac
    99721 ccatcaaaat tcctgtgaat aatccccaaa aaactgcgta atttacaaag atcatcggaa
    99781 tcccataata atcaacatgt ttaataaatg ctaatatagt cagcataata atttgaaaaa
    99841 tagtaaaatg cattatcctt actcctttat cctaattttg attgaatttc ctgtgcctta
    99901 tccgccggaa caaattgtgt aacaatttct acaccagaat ccaacaattt tttatacgtg
    99961 ctgatatttt catctgatgc ataggctcta gaagatagct gtaatgcatt atctttaaca
   100021 acagtaccgc caacaattag tcggtcaatt ctaataccgt tatctactaa atgaaggatt
   100081 gtttccggct ctttcacgat gacaaatact ttttcctgat cgtatttccc ctttttaaaa
   100141 ttcgcaatcg ctgtttcttc agtgataatt gatacagcca ttccagcagg tctagctaaa
   100201 cgcatacttg atttttggat gtcgttattt gcgacagaat catcgatcac catcactctg
   100261 tttgctttag acattggtgc ccattgcgtg acaataatcc catgcaataa acggttatca
   100321 actctcgaca taacaatact cataaatact tcctccttta tctttcattt tcttacaagc
   100381 aattaccatg ccaaaacaaa aagaacttga taaatcaagc tctttttaca taatcataaa
   100441 cgtatttaac ctcatgaatc gggatgtcga cactataata atgttcaacg acactgaaag
   100501 attgcttaaa atgtgtctga aatttttgaa attcctcact cgtttcattg aaataaattt
   100561 catcttccaa agtattttgt tttactaatc gttcaattaa gcaactaatg tgaacatata
   100621 atccgaaaca cgtattatta tcaaatgaaa ttcctagatc attttgtaag atgtatatag
   100681 catctgaaac ccgttctaat aatttagtcg gatttaatat ggtcaattgc tccattaaat
   100741 tcgttagact aaaattcttc agaatattat tgttcaaaac tttcagttca ttatcattaa
   100801 agtgtctatt caattgattg gagaatttac ttatattcat tcctaaaatc atatcttcaa
   100861 ttgaaaagaa ttctacatct tctagatgtg gatttagtgt tccaacaata aaaagaatat
   100921 tgttctcttt acgaagctcc gataaatagt tttcattcac taaagagtaa tagtcacacg
   100981 tttctatgac aagatcagta tcccttggaa agctatcttc aagtatcttt tttaatttaa
   101041 acgaagttcc taatccgctt gcacaagaac agacaatcat atccttctta atatttgatg
   101101 attcataaat ataatccaca ttttgatttt cttttacttg ttcaagaatc tgtttaagcg
   101161 gtaaatctaa taatatctga tttgcaacat caatagccat tcttgttgaa acattgttta
   101221 gtagtccgaa ttttttttga gcgtctagtt ccaaatttag ataaattttc tccagtgaac
   101281 ccatatcaac aagtaaaata atttcttcat atccggtaca ctcttgtaag tatctattaa
   101341 ttttgtctgc aatctcacta cttgaagtca ttagtggcat atcaatggcg tcgtatatat
   101401 atctctcaac gatcctatta gcagtacttg ctatacaggt tgccgttaga tctccatgag
   101461 ccaaaatcag tgctaaacgt ttttgctgaa attctttact acacattaac ttaaataaaa
   101521 taaccaaaac atttttttga atttttgtta aataaatatc ccctatttga ataaaacgat
   101581 tgactaattg aacttctcga ctacaccaac tattaatatc attgatgaag gaatataatt
   101641 tatcgtccga tgttaattgg atttgataaa tccaagaatt caaaaagata gacgttattt
   101701 gaagataata atagtcaaat tctatttgaa aatctctctc tatttcctta acatactgtc
   101761 taaatcgaat ctcttgatag gtgggatatg ttaaagctga attattttgg atatcaacat
   101821 tcttttgtct aaaatatcta tcatatttct caaaaagctg ctgcttatca gtgtctttct
   101881 tccacgtttg ataaagatac tctataagtt cgttgatatt aaaaacttcg tctacttttt
   101941 ccaaatcatc catttgaata tattttaatt cattctgtct tttcataacc ttcatgccgt
   102001 tgaaattcac ccattgtggt aaatgataca tctgaatata aagttcttcc aaatcatcta
   102061 agaaataaaa ggcattggca caggtcaatt gaatgatatt tttcaaattg ccaacatttt
   102121 cactaaagcg atagtttaat aaaaaatcta ataattgttg agatatcagt attttctttg
   102181 ctagtctttc ttcttctgat ttaaaaaata tcttgattaa ctgttttctt tcataaagag
   102241 aacgactgtc taatgttgga agtgttagct ttacaggtat tcttctcaac aaagttgata
   102301 atagtttatc ctcaggtggt tctgatgttg caaatataat ccagcaactt gatgtgtacc
   102361 agttttcatt atcacccatc cgatgaaatt tcctcgtatc cataaactga aaaagtttct
   102421 cctgacattc attgctcaat gcatgaattt catctaaaaa taagactcca ttattagcta
   102481 attctgctaa accctttctt ccatcttcgg ctcctgtata tgcacctttt acatgcccaa
   102541 aaaaattgga caagaaaagt tctggattat tggcatattc actacaatta actgaaatga
   102601 aagatgcctt ctcttctata taattattgg ccaacaaatc cttatacaca taatttgcta
   102661 aatagctttt tccagttcca gtctcgccat gaagtaaaat gggtaagcca tttggcggat
   102721 agtagattgc tgctttaatt tgagcaatta catttcgcag acttccttga cttccgataa
   102781 ccctttctat tccaacccta aaagagctat cataccattt ttccgcttcc tctgaagagc
   102841 taaaaatttg ttgatttgga actttctccg gtaaagaaaa gaagattact ggacgagtat
   102901 tgatttttcc aaattctcca tcttttacga attcattcaa atattgagag acaatattac
   102961 gactaacatt taatttttca gatattttct gtgctgtatt gttcccccat ttatttttaa
   103021 tctcagagtt ctctatgaca taattgacaa tgcgttcttt taattcttcc atcacagcac
   103081 ctcctcgaca ctattcgaat tttgccgaca gtatttttcc ataaatatga cttgctaaaa
   103141 aggctttgtt tagatcattc aattggaacg ttgcttgttt tgcttgatcc gctaagacgt
   103201 tgaaaagata ctctggcgaa atcgcatacc ccaaaatatc aacgattgtt gcaatcagat
   103261 cttcttttga ttcttcatct gaaagtaatt cacgatacaa catcgtttgt ttgcttactg
   103321 tgtcatattc ctctaaggat tcatctgcta aaagaaccac ttgttccaac aaacggtcgg
   103381 ataaatattt atagaaaatc agccccaata aataattttt atattctgac gcatccattt
   103441 tactacggag attatctgcc gcactaaata acttctgatt taattctgca cccaaaaaga
   103501 tacactccta tctcttattt aaagttgcta tataccctct tattttagca tactcagttt
   103561 tagaaaaagc aaaaaaataa agaaacttat tcttgataac aagctacgtg acgtatatcc
   103621 ctaacacaaa aatagccata tcctttggtt tggatacggc tatttgatat tactcattca
   103681 attatcaaat cacagtaggt gtgaatctca tcgggatcgg caatatctac ttctgattag
   103741 gatcaaaggt gattttctca ataaaggtgt gatacaaagc tttaacttgc ttcttctcac
   103801 tctggataag aaagcaatcc aaactagtca atatctgttg gacatctttg acttgaatcg
   103861 gttccccttg ttgttgaaag atccctaaga tttcattctc tcgaatttgt aactgtcttc
   103921 gtttttcagt caattcattt aaacgatctt ttaacatcgg aaacaattcg ggactatctt
   103981 ccaaagcagc ttcctgtttt tgctctaata gttagatttt gcgtaccatt ttcttctttt
   104041 attttccttc gattgctgat tttaatatag tctaaaggaa atttatattt attgtattct
   104101 ttataacttc aagagaactt cgtttgagta ttactgtatt tttttaattc cataagaaaa
   104161 ttagttatta cctgccacct aatcagcaaa tctgaacctg agtcaatata attaatatta
   104221 ttttccatct ccaacattgt aagtactgtt ggtgttaata tagggacttt gtacaattgt
   104281 atttttttga ttttatcatc tgcacaaagg agatttttta aatctaatcc ttcttttatt
   104341 tcaaaagttg aattttttaa atctataaga gtaaaaccaa ttccttcata ctgaatttct
   104401 gaaaatcgaa tttttctccc agttgataga tcaaggggca gaaaaccttt tattacattg
   104461 tgagtgtcac catcatttac aatgtcatta ggaattttga ttttagaatt ctgtggagca
   104521 cctattggaa gataataatt cattataatt ttgtagtctt catgaggaaa taaagtactt
   104581 ttaggattgc taagatattc tcgtaccgaa tctaagaatg gcttatccat tgaatcttca
   104641 tccccattta aactaaaaaa cattcctaca attgcttttg caactctaag tgaattcatt
   104701 ttctttattg aatgtgttaa atcagactta ccattcttta aacttgtatt gttataaagg
   104761 aaatttttat tgtcttctgc aaatctaata tattcctcaa catactcact accaaaaaaa
   104821 ctattacatt tactacataa cg
//
LOCUS       DACEEZ010000002        98288 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20313_2017
            SAMN12639966-rid9879223.denovo.002, whole genome shotgun sequence.
ACCESSION   DACEEZ010000002 DACEEZ010000000
VERSION     DACEEZ010000002.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639966
            Sequence Read Archive: SRR10023158
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 98288)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 98288)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741683.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741683.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:44:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,016
            CDSs (total)                      :: 2,962
            Genes (coding)                    :: 2,889
            CDSs (with protein)               :: 2,889
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 36 of 73
            Pseudo Genes (incomplete)         :: 29 of 73
            Pseudo Genes (internal stop)      :: 18 of 73
            Pseudo Genes (multiple problems)  :: 8 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..98288
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639966-rid9879223.denovo.002"
                     /isolate="E20313_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(1..1845)
                     /locus_tag="IWO89_000096"
     CDS             complement(1..1845)
                     /locus_tag="IWO89_000096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0454888.1"
                     /translation="MSFFNNIYNKKESFYLRIKWSIIIILSLLFLTGIAYFFYFIFSP
                     ADSNQMKEIKDSWVFYTKDDPDFKFDSKYTRTIPTVDKDETFIMETTLEKPLDEANLL
                     IKGNHQWITVYLGEKILYQRKDYQEESNPGLSLAVIDLPANYIGKKLTLTVSSPYQNY
                     AGLPPKVYLGTTGPLISFIYSQSVPQVLTMIIAVFIAIGTFFYTIYLMYKQKRLEYSL
                     ILLSCFALALGFEAVSEDILSGILFEPFVHSFLSHLFIILTSAFIICYYWSRMFYYKK
                     WYGIFCTFQLLIFIGVLLYATFTPAELPELMPIANIASVISTLVTSLACIGEAYKNNR
                     FYVACTPWIVLVAIIHCLIYIQTALGIYQTTINWSTILYIIILIVIISYNLIDHILNT
                     DKDRRQVDFLKIKNDLLEQHYEQLRQHIQEVNTLRLEFVQEMENLQDLMHTDQTKAKN
                     YVEKILEEAKNFEMLCSFCNHQVTNLILARYQKLAAKRNIQITFQADLPEELPINDDD
                     LAQLLIHALEHSFRETHAIENPLYRKIYLSIHEQEEHFVICCEHSAHYDTNLFSRGIT
                     EELDDQERFDLMMMKDVADRYTGTLTQEKDTFIDRITVQLSRNYPNPV"
     gene            2057..2575
                     /locus_tag="IWO89_000097"
     CDS             2057..2575
                     /locus_tag="IWO89_000097"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HAQ0454889.1"
                     /translation="MPDYKKITFISSITLIIFLILILLVVLVPRIFGLTPYIIEDKEM
                     SPYYAQGNLIYVRPKNTNEILVGDIITYYENSGKKISTRRVIAVENNHEDFYTKADTK
                     TYIEPGVVKKRNIIGSPIFQIPYIGLVLSKTAFAWIQGLFFTIAFCLTGITAWNTFVE
                     LKKKRRRTQKFL"
     gene            2703..3386
                     /locus_tag="IWO89_000098"
     CDS             2703..3386
                     /locus_tag="IWO89_000098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-methyltransferase"
                     /protein_id="HAQ0454890.1"
                     /translation="MRNEMMHRPVVKEELLDFMRTQQKKLPGELGKLEEEAHVAEVPV
                     IPHETVVFLKFLLGQLQPERILEIGAAIGFSSSVMATTIGENAHVTTIDRFDVMIEKA
                     KKNYERLGLTDKVTLLEGQAADILPELSGPYDFIFMDSAKSKYIEFLPECLRLLRKGG
                     VLMVDDIFQGGTILLPDEEIPRGKRAIHRKLNEFLRVVMDHPDLTSTILPLGDGVILM
                     TKESETIEL"
     gene            3695..3768
                     /locus_tag="IWO89_000099"
     tRNA            3695..3768
                     /locus_tag="IWO89_000099"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3729..3731,aa:Arg,seq:tct)
     gene            3927..4286
                     /locus_tag="IWO89_000100"
     CDS             3927..4286
                     /locus_tag="IWO89_000100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1304 family protein"
                     /protein_id="HAQ0454891.1"
                     /translation="MTILSRILVTLVAIEFFYIMYIETVRTDSDTTSRVFKMSKEELS
                     RKSVQTLFKNQGVYNGLLGVGLLYGAYLSSASKEITSMLLISIFFVALYGSLTSDRWI
                     LLKQGGLALLALLSIFL"
     gene            4409..6040
                     /locus_tag="IWO89_000101"
     CDS             4409..6040
                     /locus_tag="IWO89_000101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SulP family inorganic anion transporter"
                     /protein_id="HAQ0454892.1"
                     /translation="MFKNYVSSLKKEFSGYDLSRFQKDLLAGITVAAVALPLALAFGV
                     SSGADAAAGLITAIIAGIVIGGLSGGFYQISGPTGAMAAILMSIAATKGMQGVLLATF
                     LAGLFLLAAGLFRLGTLTSFIPAPVITGFTSGIAIIIALGQIDNLFGVHSEGTNVIEK
                     LSSYQTIGFPINTASLIMGGLVILGMFIYPKKWAKRMPSSLLAIILATAVMLLFHLPV
                     ATVGKIPQTLISSNRLNMGDFSFSALQQVAVPAISIALLGMIESLLCGASAGRMANKP
                     LDSNQELMAQGIGNLLLPFFGGIPATAAIARTSVAIKSGAQTRLAGMIHAVVLLLSML
                     VFAPVMSQIPMPALAGVLIVTAWRMNEWETIKELFTNKYWSAVLLFILTMICTVIFDL
                     SIAIVIGVIGGCVFFVVKSAAITISLETIDWQRMGLPETEKLNNWTVVYISGPLFFMS
                     SEKLKNKLEELNHHDGVIFSMRGVPSIDLTARAVFDEFQEKAEQRGQTIIYTSLQPEV
                     EKQLSHLWEHTFTHQHQTVAHALTALHAKYTAISH"
     gene            complement(6102..6743)
                     /locus_tag="IWO89_000102"
     CDS             complement(6102..6743)
                     /locus_tag="IWO89_000102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HAQ0454893.1"
                     /translation="MEYPKELIQEASLRMNGRPVEGFIAGQGPLHPKLMLVGEAPGKT
                     EIDTHVPFSGQAGKELMQALSSTGLTREEVYITSAVRSRPYRVTHRINKRTQQSETVY
                     PNRTPTRSEVFAHAPILDYELMKVQPKLIATLGNIGLQRLLGKEYTVTKNHGQLYTGP
                     VLQLTEQKDAYEWSEKNYRILPLFHPAAIFYNRSLVPVIHQDWQVLASLLQQY"
     gene            7025..7333
                     /gene="rplU"
                     /locus_tag="IWO89_000103"
     CDS             7025..7333
                     /gene="rplU"
                     /locus_tag="IWO89_000103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002355866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HAQ0454894.1"
                     /translation="MYAIIKTGGKQVKVEVGQAIYVEKLDVEAGEKVVFDEVILVGGE
                     STKVGAPTVAGATVEGTVEKHGKQKKVVTFKYKPKKHSHRKQGHRQPYTKVVIDAINA
                     "
     gene            7360..7704
                     /locus_tag="IWO89_000104"
     CDS             7360..7704
                     /locus_tag="IWO89_000104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal-processing cysteine protease Prp"
                     /protein_id="HAQ0454895.1"
                     /translation="MIKGTFKRNDAGQIVSFTLTGHADAGPYGSDIVCAGVSALAIST
                     VNGIASLAGFEPIVEMNEEEGGYLYTEVTSGMTQEQNNIAEILLENLLLGLQSIEAEN
                     SEYIQIKTINDK"
     gene            7717..8010
                     /gene="rpmA"
                     /locus_tag="IWO89_000105"
     CDS             7717..8010
                     /gene="rpmA"
                     /locus_tag="IWO89_000105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HAQ0454896.1"
                     /translation="MLLSMNLQLFAHKKGGGSTSNGRDSESKRLGAKRADGQTVTGGS
                     ILYRQRGTKIYPGVNVGIGGDDTLFAKVDGVVRFERKGRNKKQVSVYPVAQEA"
     gene            8417..11251
                     /locus_tag="IWO89_000106"
     CDS             8417..11251
                     /locus_tag="IWO89_000106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008266983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAQ0454897.1"
                     /translation="MAKRIDRIYMYVKEKTAHLTKAEYDQGVTTQEIAEVLGIQRTNS
                     SKDLNQLVREGKLIKTDGRPVRYIYQTLVTHVKPVTKHVVSYKETPVSEEKNTLQIDT
                     KDIFAKIIGANGSMKNSVEQAKAAILYPPRGLNCLITGPTGSGKTYFAHAMYHFAKAH
                     HVIDEETELIVFNCADYANNPELLMSHLFGYAKGAFTGADEEKMGIIDQADGGMLFLD
                     EIHRLPPEGQEMIFYFMDHGTYSRLGETTKSHEANVRIVGATTEDPGSSLLETFVRRI
                     PINIKLPAFEKRPANEKVDLVKIMIAHEANRTQRKISLTEDVAKALIGSVTYGNIGQL
                     KSNIQLVCARGFLNHMQSPEISITIDDLTEGIRSGLIQLASNRSAMAELSKILEPKIT
                     VAPNDTIMKIQSDSYELPYNLYDIIGDKAALLKSDGLDQEAINHFISTDINIHLKSFY
                     KDHGFSFNADSKLAEFVDPKIIEVTNQIYVMVKRSLPYEFQQNFIYAMSLHISSFLKK
                     INLGEERHTNDNIREMAFDFPDEYAVAKEVRSYIETYFQVEIPESEDYYLTVLLVSLR
                     ANQASGRIGVVVAAHGNSTASSMVQVVQQLLDAENVRAVDMPLDMDPKTALDRIERNV
                     QEVDEGSGVILLVDMGSLASFNSQIQRDTGIPVRTVDMVTTSLVLETVRKVSVLGTDL
                     DMLHESLKNFRGYAEISSVESVQEAKPIHKKKAVLAICASGEGTAQRIKELIERAVNK
                     RQETELEVLALSIVEVKEELPVIQKEYQIIATTGITDLNIQAPFIPLERFIDQNIEVI
                     LDQLLMESELEETEFISLDEESAKNTCVEFISDNFIFINGSKLIDPMWQFSTQISQTT
                     GIGDEEYGFKINLVMHTAGMIERIIRNEPLTVEENELTNTTNDPLYSQLAASVVLLED
                     QIKVKVPIEEMYYLLRLVHNQLDKKEYTVP"
     gene            11442..11933
                     /locus_tag="IWO89_000107"
     CDS             11442..11933
                     /locus_tag="IWO89_000107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit IIB"
                     /protein_id="HAQ0454898.1"
                     /translation="MDIRLARIDDRLIHGQVATVWSKQTGVERIIVISDVVAQDKLRK
                     FLLKEAAPPGITANVITVQKMIQIYHSGLLSRAKVMLLFTNPQDVEAVVRCGVQLKSL
                     NIGGMSFTEGKSMITNFVSVNQQDIDSFRYLNSQEVELEIRKVPADRKILLMELLAKI
                     KKV"
     gene            12036..13025
                     /gene="manX"
                     /locus_tag="IWO89_000108"
     CDS             12036..13025
                     /gene="manX"
                     /locus_tag="IWO89_000108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IIAB"
                     /protein_id="HAQ0454899.1"
                     /translation="MVGIILASHGQFAEGILQSGSMIFGEQENVKAVILKPSEGPDDL
                     RAKLEEAVASFDNQDEVLFLVDLWGGTPFNQSNTLFEEHKDKWAIVSGLNLPMLIEAY
                     ASRFSMESAHEIAAHIIETAKDGVKVKPEELEPAEAPKAAVEDAQPKGALPEGTVVGD
                     GKIKYVLARVDSRLLHGQVATAWTKAVQPNRIIVVSDAVSKDDLRKRLIEQAAPPGVK
                     ANVIPISKMIEVAKDPRFGNTKALLLFENPEDVLTAVEGGVDIKELNVGSMAHSVGKV
                     VVSKVLSMGQEDVEAFEKLEQKGVKFDVRKVPNDSRDNMDDILKKAKAELAKA"
     gene            13058..13861
                     /locus_tag="IWO89_000109"
     CDS             13058..13861
                     /locus_tag="IWO89_000109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_463630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose/fructose/sorbose transporter subunit
                     IIC"
                     /protein_id="HAQ0454900.1"
                     /translation="MSIISIILVVLVAFLAGMEGILDEFQFHQPLVACTLIGLVTGNL
                     EAGIVLGGTLQMIALGWANIGAAVAPDAALASVASAIILVLGGQGVKGVPSAIAIAVP
                     LAVAGLFLTMIVRTIAVPIVHLMDAAAEEGNIRKVEMWHIIAVCLQGVRIAVPAAALL
                     FIPAQTVQSFLESMPAWLTDGMAIGGGMVVAVGYALVINMMATKEVWPFFVIGFVVAA
                     ISQLTLIALGALGVALALIYLNLSKMGGSSNNGGGSNSGDPLGDILNDY"
     gene            13882..14796
                     /locus_tag="IWO89_000110"
     CDS             13882..14796
                     /locus_tag="IWO89_000110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose/fructose/sorbose transporter family
                     subunit IID"
                     /protein_id="HAQ0454901.1"
                     /translation="MAEEKIKLSKKDRLAVAWRSTFIQGSWNYERMQNGGWAFSMIPA
                     IKKLYKTKEERSAALKRHLEFFNTHPYIASPILGVTLALEEERANGAPVDDVAIQGVK
                     VGMMGPLAGVGDPVFWFTVRPMLGALGASLAMGGNILGPIIFFLGWNIIRWAFMWYTQ
                     EFGYKAGSKITDDLSGGLLQDVTKGASILGMFVLAALVQRWVSIKFLPVVSTVKLDKG
                     AYIEWDKLPSGGEGIKSAFEQVNNGLALSPEKVTTLQDNLDQLIPGLAALGLTLLCMW
                     LLKKKVSPIVIILGLFVVGVVGHVIGLL"
     gene            14879..15253
                     /locus_tag="IWO89_000111"
     CDS             14879..15253
                     /locus_tag="IWO89_000111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF956 family protein"
                     /protein_id="HAQ0454902.1"
                     /translation="MVQSINTKVDLVIDATSFTGLTDYGQIMIGDNGFEFYHARDRRK
                     YIQIPWEEVDYVLASVMLKGKWIPRYAIQTKKNGTYTFSSKEPKKVLRAIREYVDEDR
                     MVQSLSFFDVVKRGIRSMFKRK"
     gene            15392..15637
                     /locus_tag="IWO89_000112"
     CDS             15392..15637
                     /locus_tag="IWO89_000112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HAQ0454903.1"
                     /translation="MSWLWTLIVGAVIGAIAGAITSKGNSMGCVTNIIAGLVGSSIGQ
                     AIFGEWGPKAAGMALIPSILGAVILVAVVSFFFGKRS"
     gene            15809..16873
                     /locus_tag="IWO89_000113"
     CDS             15809..16873
                     /locus_tag="IWO89_000113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HAQ0454904.1"
                     /translation="MMLRVEKLRKKMQEENLDSFLVTSPYNLRYLTNFTGTTGLAVIT
                     LEKAFFITDFRYTEQAAAQAQGFEIIKNVGPIFDEVANLVRKEELHSLAFEETTVSFL
                     EYSVLEEIIDAELVPVSQMIEELREVKDEEEIAIIEKACHIADMAYDHILKMIQPGMT
                     EIEVANQLDFYMRSLGASGVSFDTIVASGLRSAMPHGVASKKIIEQGDLITLDFGCYY
                     EGYVSDMTRTFAIGDPGEKLKEIYQIVLDAQLAVIDAAKPGMTGVQLDAVARDYISKH
                     GYGEAFGHSTGHGIGLEIHEGPNVSSRAEKQFVPGNVITDEPGIYLPGIGGVRIEDDL
                     LITEDGNRVLTRSPKELIIL"
     gene            17119..17544
                     /locus_tag="IWO89_000114"
     CDS             17119..17544
                     /locus_tag="IWO89_000114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HAQ0454905.1"
                     /translation="MADEKNLILNANQDLGEIVIAPEVIEVIIGIAASKVEGVYGMRG
                     TFASNVTELLGRAAHGKGVYLANEEDGLKVDLYCYLEYGVSVPKVAMEMQERVSQQVL
                     FMTDIDLSEVNIHVVAVVPEKLEQPTIEELFEDEEEENE"
     gene            17537..17986
                     /gene="nusB"
                     /locus_tag="IWO89_000115"
     CDS             17537..17986
                     /gene="nusB"
                     /locus_tag="IWO89_000115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HAQ0454906.1"
                     /translation="MNKELSRHEIREMALQALFPLDFNADLTKEDAIFNAIELDHRDM
                     INEDESEFVPVYLDTLVGGVCAKKDELDKVIEKHLKNNWHINRISKMDLVILRIAIFE
                     MTYVTDVPAAVALNEAIELAKTFSDDRSRKFVNGVLSNVLKELEAGA"
     gene            18083..18937
                     /locus_tag="IWO89_000116"
     CDS             18083..18937
                     /locus_tag="IWO89_000116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional methylenetetrahydrofolate
                     dehydrogenase/methenyltetrahydrofolate cyclohydrolase"
                     /protein_id="HAQ0454907.1"
                     /translation="MAELINGKELAEKMQAEIAEKIQKLKDNGIHPGLVVLLVGENPA
                     SQIYVRNKERVAKEIGIHSLVERYPETISEDELLAEIEKYNQDPSFHGILVQLPLPKH
                     IDEEKILLAIRPEKDVDGFHPMNLGRLLAGKPDKIPCTPYGIMKMFEAYSIDPAGKRA
                     VVIGRSNIVGKPMAQLLLMADATVTVAHSKTENLAELAQEADILVVAIGRGHFVTKEF
                     VKPGAVVIDVGMNRDANGKLIGDVKFDEVEPIAGWITPVPKGVGPMTITMLMYQTVKS
                     AEAFGAGE"
     gene            18938..20275
                     /locus_tag="IWO89_000117"
     CDS             18938..20275
                     /locus_tag="IWO89_000117"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HAQ0454908.1"
                     /translation="MAQEYLTVTTLTKYLKRKFDADPYLERVYLTGEISNFRLRANAH
                     QYFSLKDDHAKISAIMFKGAFQKLRFQPKEGMKVLVVGRISLYEAGGSYQIYVEHMEP
                     DGIGALYQELEERKEKLMKEGLFQGPKKMLPRFPKRIAVLTSPSGAVIRDIITTVKRR
                     YPIVQLVLFPTVVQGNQAADNVVRNIQRVEEMGNFDTMIIGRGGGSIEDLWPFNEERV
                     ARAIYQANTPVISSVGHETDTTIADLVADVRAATPTAAAELAVPVLSEELMRIQERQA
                     RLQQAFVRQIQRKQERFERARRSYVFRQPERLYEGQTIKLDQLKQRLYQATQQIYHAK
                     EKQTIALSHQLEQVAPLYRVKAARQETAYLEKRLEEKIQQYMKQQQQRFAQAVQSLDL
                     LSPLKIMGRGYSYTTLNDQVVKSVTEIHQGDLLTIHYQDGIVKGQVQEIDKGE"
     gene            20280..20504
                     /locus_tag="IWO89_000118"
     CDS             20280..20504
                     /locus_tag="IWO89_000118"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HAQ0454909.1"
                     /translation="MENPTFEESLQELEKIVMQLEQGDVPLESALDSFKRGMELSKHC
                     QDTLAKAEETLTKMMTESNEEVDFDGNEES"
     gene            20506..21396
                     /locus_tag="IWO89_000119"
     CDS             20506..21396
                     /locus_tag="IWO89_000119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HAQ0454910.1"
                     /translation="MRLTDFSAIHLPAVEKEILSFLDEHTTDRGLYDAMSYSVKAGGK
                     RIRPLLLLTAVASFDEPIDVPVYQVAAALEMVHTYSLIHDDLPAMDNDDLRRGKPTNH
                     KVFGEALAILAGDGLLTGAFQLISMAHLGNSPKLLLLQQLAVCAGSQGMVAGQAADIE
                     GESKKLSLEELAFIHERKTGHLIRYALLAGGILAKQPEEILLLLQRLAEHLGLAFQIR
                     DDLLDVIGTTKTLGKTAGKDERMEKNTYPRLLGLEKTREALEIELLSANKIIDKLEED
                     VFSFDGGLMRQMIKQFDVEK"
     gene            21405..22223
                     /locus_tag="IWO89_000120"
     CDS             21405..22223
                     /locus_tag="IWO89_000120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TlyA family RNA methyltransferase"
                     /protein_id="HAQ0454911.1"
                     /translation="MEKERVDVLAVKQGLFETREQAKRSVMAGLIYNEKNERFDKPGE
                     KIPVSSELRVKGKKLPYVSRGGLKLEKALKAFDLSVEGKTLLDIGASTGGFTDAALQN
                     GAKMSYALDVGYNQLAWKIRQDPRVVVMERVNFRYAKPEDFTLGIPEVAVIDVSFISL
                     KLMLPPLHAILKEEGEVIALIKPQFEAGREAVGKNGIVRDPQTHQKVLEDILSFAAQG
                     GYDVLELSYSPITGGEGNIEFLAHLRKVPESGTINSAINMAEVVSNAHEQFDHK"
     gene            22330..22779
                     /locus_tag="IWO89_000121"
     CDS             22330..22779
                     /locus_tag="IWO89_000121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine repressor"
                     /protein_id="HAQ0454912.1"
                     /translation="MRKKDRHRLITRLLSEQDIRKQEEFVEILKSKGISVTQATISRD
                     IKELKLIKVPASDGGYRYSIPAETNEDVNTKLEKLLKDAFVAVDQMEKFVILKTLPGN
                     ASAAANLIDKRYKKELFSIINDDDNVLMITRTEDQAVLLKKDFLHYL"
     gene            22797..24479
                     /gene="recN"
                     /locus_tag="IWO89_000122"
     CDS             22797..24479
                     /gene="recN"
                     /locus_tag="IWO89_000122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecN"
                     /protein_id="HAQ0454913.1"
                     /translation="MLQEISITNFAIIPELRLSFHEGMTALTGETGAGKSIIIDALGL
                     LAGGRGSSDYIRQGAEKCVLEGLFELPKQEGFSELMVELGIETDEDNLIVRRDMSLTG
                     KNVCRVNGHIITLANLRRIGSYLVDIQGQNEHQELLQPESHLALLDRFGDVAFQQKKK
                     SYQQEYISYRELEKRVRKVQQNEKSYVQRIDMLHFQQEEIAAADLQVGEEEKLKEERE
                     KLSNYQKIADGLAAGYGALTNNEQNSVDGVGLAVSELQGIAHLDVEYEAIYENIQSAY
                     YLLQDAIGDMSRQIDLLELDESRLEEVTQRLELIRQLKRKYGESIESILAYYDEITEE
                     LASSDFSEGQLDKMKEELEQKELLLQQQAADLHEARKKIAKELEKSILHELKSLYMEN
                     TEFEVRFLKEENRQLNHDGFDQIEFYITTNPGEPLKPLVKVASGGELSRMLLALKTIF
                     SSEQGVTSIIFDEVDTGVSGRVAQAIADKISQISKYSQVLCITHLPQVAAVADYQYYI
                     VKAVIDGRTQTSVSELKTKEREEEIARMLAGSEITKLTLEHAKELLKLAKRT"
     gene            complement(24534..24650)
                     /locus_tag="IWO89_000123"
     CDS             complement(24534..24650)
                     /locus_tag="IWO89_000123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4044 domain-containing protein"
                     /protein_id="HAQ0454914.1"
                     /translation="MREKKQTSTFAKVTKFVIWTMLILTIGSVVLTAVMSVM"
     gene            complement(24723..25679)
                     /locus_tag="IWO89_000124"
     CDS             complement(24723..25679)
                     /locus_tag="IWO89_000124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HAQ0454915.1"
                     /translation="MIKYFTLDNEKLRNTGKETDQTIWYCVERPNQEEIHQLTHQFEI
                     PSDYITSILDDAENSRTEEFNQSKLTKPALLLLQYPFAKTSPSGYFQVDTYPLSIILT
                     PKKKLITITNHEPLFLKKVFSQTFPKSDISPNLNIFFQFIWQVTLSYNSYLATLQSQC
                     DTLEAKLQVSTENKQLYQIMDIQKSLVYFREATSANLDALKELAQNKLFQENHALKNH
                     LRDVLVETEQAMTTSRIQLKLVEQMNQTFSAIVSNNLNNIMKILTSLTIILTIPTIVG
                     SIYGMNIKLPIAERDDAFIWLVLLTLFICIVTTYYLKKGKFL"
     gene            25850..26218
                     /locus_tag="IWO89_000125"
     CDS             25850..26218
                     /locus_tag="IWO89_000125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3397 domain-containing protein"
                     /protein_id="HAQ0454916.1"
                     /translation="MGTFTPLILFWYIYPVIVLFGCQFLVKLFSLNRRFKIKAPDLAI
                     PFLWGGMHALSRNTGTISILPFFFISVLLMGICIAVFQAYYYEEIIYPRYFKMTWRLV
                     FLLTIVLYAVLIIVNILSYL"
     gene            26388..26819
                     /gene="mraZ"
                     /locus_tag="IWO89_000126"
     CDS             26388..26819
                     /gene="mraZ"
                     /locus_tag="IWO89_000126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010754126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="division/cell wall cluster transcriptional
                     repressor MraZ"
                     /protein_id="HAQ0454917.1"
                     /translation="MLMGEFQHNIDAKGRLIVPSKLREELGEKFVLTRGLDGCLFGYP
                     MSEWENLEAKLNEMPLAKKDARTFVRFFYSAATECELDKQGRINIPSTLRNYAALTKG
                     CVVIGVSNRIEIWDETRWQDFSAAAEENFDEIAESMIDFGL"
     gene            26840..27799
                     /gene="rsmH"
                     /locus_tag="IWO89_000127"
     CDS             26840..27799
                     /gene="rsmH"
                     /locus_tag="IWO89_000127"
                     /EC_number="2.1.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HAQ0454918.1"
                     /translation="MTETFQHYTVMLKETVDGLNIKPDGVYVDCTLGGAGHSEYLLSQ
                     LSSKGHLYAFDQDQKAIDHAKLRLASYVEQGMVTFIKANFRELEERLSEHVDKVDGIL
                     YDLGVSSPQLDEAERGFSYHQDAPLDMRMDQSAPLSAYHVINEYSYHELVKIFFRYGE
                     EKFSKQIAREIERVRKTKPIQTTGELVEIIKQVIPAPARRKGGHPAKRIFQAVRIAVN
                     DELGAEEASLEQAIRLLKVNGRISVITFHSLEDRIVKSMFKEYSTVQDLPPGLPVIPD
                     EFQPELKVITRKPILPSEEELTENNRSRSAKLRIAEKIRMKEE"
     gene            27804..28205
                     /gene="ftsL"
                     /locus_tag="IWO89_000128"
     CDS             27804..28205
                     /gene="ftsL"
                     /locus_tag="IWO89_000128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsL"
                     /protein_id="HAQ0454919.1"
                     /translation="MAELKKMQDYEYDLPELVDQPEQQMPDSSSNQEIILPQSPAKRL
                     KQISALEKIAVASVAIAIVALCVLTVMLRTNISGAEKEITSIQTEINDKKQEKTSLEQ
                     EKNELSRTERIKQIAEEKGLSINDDNLRKVK"
     gene            28206..30398
                     /locus_tag="IWO89_000129"
     CDS             28206..30398
                     /locus_tag="IWO89_000129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein"
                     /protein_id="HAQ0454920.1"
                     /translation="MSLKNKIRRFMEKKNLNPMNNRKKVGIILFATSIGLFFLFAVRF
                     SYIVIGGHVAGTSLAEKTKQLYQGSEVVKAKRGTIYDRNGVALAEDASSYSIKAILSK
                     TYTSGDKKLYVEEKNFDKIAEILHKNLSIDKKDALNILEDGAKKELYQVEFGSYGKNI
                     SQETKQNIEADMKKEGVAGLYFVDHQARMYPNGVFSSHFIGYAVPDKDENGLVGKLGL
                     ESAYNDILSGKDGKIIYQKDNFQNPLPGTVAEEEKAVDGQDIYTTLDSRLQSYLETLM
                     DQVNEEYQPEELTAVLMKAKTGEILAMGQRPTFNPETMEGLTGKDAIWRNFLVQDSYE
                     PGSTMKVFTTAAAIEEGEFNENETFQSGKIQVEDATINDHDFGEKGVLTMRQALSWSS
                     NVGMVILEQRLGGRWYNYLQKLGFGQSTHSGLDDEVNGALPTLNIVDRAMSAYGQAVG
                     VTNFQMMKAFTSIANNGTMIQPRYISKVVDPQTGEERTTQTEVLGQPFSKETTEKVRE
                     YMRDVVESENYGSAYGVYSVPGYNVSAKNGTAQIASDTGGYQTGDTAYLYSIVEMVPS
                     EDPDYVLYLTMKHPKTYDRMALAKIANPLMKRAMDFKETEEDSDTETKTEKVSVADYR
                     NLEADVAAADAQKSGLQPVVIGNGKKVQKQSTANGDQLISGEKLILYTGGDKLMPDVT
                     GWSKADIMKLGKILGIEVSFDGDGYCVKQELAPYEKITEDKLSFTLEE"
     gene            30424..31386
                     /locus_tag="IWO89_000130"
     CDS             30424..31386
                     /locus_tag="IWO89_000130"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HAQ0454921.1"
                     /translation="MEWTQALIPIVSSCAMTIAAMPLFIGYFQMKKQGQAIREEGPKW
                     HNSKAGTPTMGGLVFLIGSILTGIWVGAWQKQLTPTLFILLFVLALYGVIGFLDDFIK
                     IFKKRNMGLNSKQKLIGQILGGIIFYLVYRSEGYPGVLNFFGLDLPLGIIYGIFALFW
                     LVGFSNAVNLTDGIDGLVAGLGSISFAAYGLIAWHQHQYDVLIICLSVLGGLLGFFVY
                     NRKPAKIFMGDVGSLALGGLLAAISIMLNQEWTLLLIGLIYVMETASVMLQVTSFKLT
                     GKRIFKMSPIHHHFEMCGWSEWKIDTVFWLISIVTSLITLWIVW"
     gene            31400..32761
                     /locus_tag="IWO89_000131"
     CDS             31400..32761
                     /locus_tag="IWO89_000131"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HAQ0454922.1"
                     /translation="MKKISTYENKKVLVLGLAKSGVSAAKLLHELGALVTVNDGKPFD
                     ENPEAQELLSLGIKVITGSHPIELLDEEFSLMVKNPGIPYSHPLVAKAQEMGIPVITE
                     VELAYEVAECPIIGITGTNGKTTTTTMTGLLLNAGADQGIARLAGNIGYPASGVAQEA
                     KSEDKIVMELSSFQLMGITDFRPHIAVITNIYEAHIDYHGTRKEYVKAKWNLQKNMTE
                     KDYLILNWNQSELQELAQRTKARVLPFSTKEVLEDGVYADDYSIYYKKEKIMEISELG
                     VPGKHNVENALAAISVAKLYGISNEAIRETLHFFHGVPHRTQYVGEIQGRKFYNDSKA
                     TNILATKMALSGFETSKVVLLAGGLDRGNTFDELIPSLKGIKAMVVFGQTKEKLMDAG
                     KKAGIETIVTADSVEQAVPLALENSTDGDVVLLSPANASWDQYPNFETRGNRFMEAVN
                     RLK"
     gene            32787..33875
                     /gene="murG"
                     /locus_tag="IWO89_000132"
     CDS             32787..33875
                     /gene="murG"
                     /locus_tag="IWO89_000132"
                     /EC_number="2.4.1.227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase"
                     /protein_id="HAQ0454923.1"
                     /translation="MKILVTGGGTGGHIYPALAFVNYVKTQEPNAEFMYVGAKRGLEN
                     KIVPDTGMPFHTLEIQGFKRKISMHNIKTVRLFLKSIRQAKKILSDFQPDIVIGTGGY
                     VSGAVVYAAAKMGIPTVIHEQNSVPGVTNKFLSRYVDRIALSFKDAASFFPENKAVLV
                     GNPRAQEVADTKKSDILKTFDLDPEKKTVLIFGGSQGALKINQAVSVFLSEYIPSSYQ
                     VLYASGDRYYEEILNKINPEIQNISVQPYIKNMAEVMASSDLLVGRAGATSIAEFTAL
                     GLPAVLVPSPYVTNDHQTKNAMSLVHAGAAKMIADNELTGESLSQTVNEIMGDEELQK
                     QMCRASKEQGIPDASKRLYDLVKQIIKK"
     gene            33890..35110
                     /locus_tag="IWO89_000133"
     CDS             33890..35110
                     /locus_tag="IWO89_000133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsQ-type POTRA domain-containing protein"
                     /protein_id="HAQ0454924.1"
                     /translation="MSKKKEPKKLSHPSSDENNLTPWQKANRKYLAEQEMKNQEPKKE
                     DITLDTPLSDSEEEKTVQEEQQEESQSEKKIEFFEEIKQKKNGGPYNGSFLNRLPNLK
                     SQRHKVLFRRLALIITVLGIPLIFLIYYVSPYSKLQAVAVSGNKTVNSQEIISDTKLS
                     LGENVWGQYFHRSTYIDRLKKAQPRIETANIHFKGMNEFDLDVTEYKEIALIAKNNQY
                     YPVIENGTVLDEKVANPTKNLPILEEFKDSAKIKELTKQYNQLSSELQKAISEIKYTP
                     RASNKNLIQLNMNDGNQVIVNINNLANQMKYYSQVAKDMDEKGVIDMEVGIFSHPYGS
                     TQETEESGQSEETTESEENADQTNESSEEAETQKAQTPGSSSTNGSSDSNDSSETSQI
                     EDNPSTTPSSLNEN"
     gene            35266..36591
                     /gene="ftsA"
                     /locus_tag="IWO89_000134"
     CDS             35266..36591
                     /gene="ftsA"
                     /locus_tag="IWO89_000134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsA"
                     /protein_id="HAQ0454925.1"
                     /translation="MAKTGMYVGLDIGTTSVKVVVAEYIDSQMNIIGVGNAKSEGINR
                     GIIVDIDKTVQAIQRAVRQAEEKAGIQIKGVSVGLPANMLEVENCQGMIAVNGDSKEI
                     TDEDVRNVASAALVRSIPPERQIVSILPQDFTVDGFEGIKDPRGMIGVRLEMYGLLFT
                     GPKTIIHNIRKCVENAGLIVNEMVITPLALTESILSDGEKDFGTIVIDMGGGQTTTAV
                     MHDKQLKFTNLDQEGGEFVTKDISIVLNTSFNNAEALKINYGDAYPERTSPDEEFPVD
                     VIGQSEPVKVDERYLSEIISARMEQIFNKAKDALDQIEALELPGGVVLTGGAASLPGV
                     VDLAQEIFGVNVKLYVPNHMGLRNPVFTNVISIVDYSANLSEVYQLAKIAVTGETSAA
                     RQMVVEQETTNTYESYEAPEETIYDEPEPKESGENVKNKIKGFFTNIFD"
     gene            36614..37855
                     /gene="ftsZ"
                     /locus_tag="IWO89_000135"
     CDS             36614..37855
                     /gene="ftsZ"
                     /locus_tag="IWO89_000135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HAQ0454926.1"
                     /translation="MEFSIDNNINDGAVIKVIGVGGGGGNAVNRMIEENVKGVEFITA
                     NTDVQALKNSKAETVIQLGPKYTRGLGAGSQPEVGQKAAEESEQSLREALDGADMIFI
                     TAGMGGGTGTGAAPIVAGIAKELGALTVGVVTRPFTFEGPKRGRFAAEGIARLKENVD
                     TLLIISNNRLLEVVDKKTPMLEAFREADNVLRQGVQGISDLITAPGYVNLDFADVKTV
                     MENQGTALMGIGVASGEDRVVEATKKAISSPLLETSIDGAEQVLLNITGGLDMTLFEA
                     QDASDIVANAATGDVNIILGTSINEEMGDEIRVTVIATGIDESKKERKSSRPARQAQM
                     QSPAQKTVLDMDQAKPTSSEEENSFGDWDIRREQNVRPRVDDSNFDNIEKKEFDTFNR
                     EETKSHGDDELNTPPFFRRKK"
     gene            37872..38549
                     /locus_tag="IWO89_000136"
     CDS             37872..38549
                     /locus_tag="IWO89_000136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggS family pyridoxal phosphate-dependent
                     enzyme"
                     /protein_id="HAQ0454927.1"
                     /translation="MIADNLQKIKQEIQDSCALVHRDPKEVTLVAVTKSVESAQAEEL
                     VRAGVMNCAENRVDKLLEKKQDLFDFPEIKWHLIGNLQRRKVKLIVNEIDYFHALDSL
                     RLAEEIQKRLEKELSCFIEVNVSGEESKHGISPDELLPFVESLAACTKIKVVGLMTMA
                     PKEASDEQIHKVFGTLKELRDLVQAKGYAHAPCSELSMGMSQDFQIAIEEGATFIRVG
                     TALFKDE"
     gene            38563..39162
                     /locus_tag="IWO89_000137"
     CDS             38563..39162
                     /locus_tag="IWO89_000137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein SepF"
                     /protein_id="HAQ0454928.1"
                     /translation="MPLLNRGAIANFFGLADEEEYEYQDYPETTKKAVQPTKKNTTQN
                     QSTHSVSAANTHEKQKTATSRPVTHESIQPAEKKKVIELHQSPTKTVAKERTASRTST
                     QPKANLAGKITVMEPRAYSEAMAIAKRIIAGEATLVNFHLVEEKQARRIVDFLTGTVY
                     ALDGDIKRVGDEIFLCTPTGMEIDSSTAQSFADANLFDL"
     gene            39177..39464
                     /locus_tag="IWO89_000138"
     CDS             39177..39464
                     /locus_tag="IWO89_000138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007207099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggT family protein"
                     /protein_id="HAQ0454929.1"
                     /translation="MYTIALLLHLLQRAAYFYSIILMVYALLSWFPGGYQSGLGRLLA
                     KICEPYISLFDRLPLRIGPLDFTILGAILILNLAVQGLAQIVIWITVHLVY"
     gene            39513..40295
                     /locus_tag="IWO89_000139"
     CDS             39513..40295
                     /locus_tag="IWO89_000139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein"
                     /protein_id="HAQ0454930.1"
                     /translation="MDANVYQHFRADERTFIDSVGDWIEQVQGQYAPYLTEFLDPRQS
                     YILETLIRQSSDLRFMFYGGYEQAERKRCLIFPDYYEPVEEDFETALYEIHYPSKFAS
                     LSHGKILGTLVGTGIKRSYFGDIISDGDRWQVFIANEVDHFVAAQVTKIGKVAVRLEP
                     IKYTEMIMPKDSWTQERGTVSSMRLDSVISEIYNISRQRSKQLIEAGKVKVNWTENTR
                     PDFVLELLDIISVRGYGRVQIQELEGKTKKEKYRVLFGVLRK"
     gene            40316..41023
                     /locus_tag="IWO89_000140"
     CDS             40316..41023
                     /locus_tag="IWO89_000140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DivIVA domain-containing protein"
                     /protein_id="HAQ0454931.1"
                     /translation="MALTPLDIQNKTFPTKMRGYNQDEVDDFLDLVVRDYEELTQRNR
                     ELEKAVKHSEEKLEYFNELKDALNQSIIVAQDTADKVKTSASKESEVIVTSAQNKADE
                     LVANAEKRAHQLTTDAEEKARKILTDATEKARQLATETEDLKKKTRVFHQRISLMLES
                     QLEQVKSPEWDEILQPFSSYVTDSHEVIKEVLAKQLDNENDTDVNSDTGVIEAPMTAF
                     DTQAIDISIVPGENENE"
     gene            41285..44083
                     /gene="ileS"
                     /locus_tag="IWO89_000141"
     CDS             41285..44083
                     /gene="ileS"
                     /locus_tag="IWO89_000141"
                     /EC_number="6.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoleucine--tRNA ligase"
                     /protein_id="HAQ0454932.1"
                     /translation="MKMKETLQLGKTKFPMRGNLPNREGEWQKEWEENRIYQKRQELN
                     EGKPSFVLHDGPPYANGNIHLGHSLNKISKDIIIRSKSMSGYRAPYVPGWDTHGLPIE
                     QVLANKGVKRKEMSMAEYLEKCREYALSQVDKQRADFKRLGVAGDWEHPYVTLDPSYE
                     AAQVRVFGKMAEKGYIYKGLKPIYWSPSSESSLAEAEIEYKDVKSPSIFVAFNVKDGK
                     DILDEDTAFVIWTTTPWTLPANQGIAVNPKFTYVLVEADGRKFVVAKDLLETVQSEIG
                     WENVTVLKEFAGQEMEYMTASHPFYERESLVILGDHVTLDAGTGLVHTAPGHGEDDYI
                     AGSKYKLSVVSPVDSKGLFTEEAPGFEGIFYDKANPMITDLLKEKGALLKLDFFTHSY
                     PHDWRTKKPVIYRATPQWFASIDKFRQNILDEVEKVEWVIPWGKTRLYNMIRDRGDWV
                     ISRQRAWGVPLPIFYAENGEAIITPETIEHVAKLFAEHGSIIWFEKEAKELLPEGFTH
                     PGSPNGEFTKEKDIMDVWFDSGSSHEAVLRQRPELSFPADMYLEGSDQYRGWFNSSIT
                     TSVAINGVAPYKSVISQGFALDGEGRKMSKSLGNIIAPEKVIKQFGADILRLWVSSVD
                     YEADVRVSMDILSQVAEVYRKIRNTMRFLLANTEDFDPKKDTVSYNDLRSVDKYMTVR
                     LNQVIKEIREEGYDKYNFMHIYRTVMNFLTVDLSSFYLDFAKDVVYIEAEDDYQRRCM
                     QTVFYQTAVALTKLLTPIIPHTAEEIWSFLKEEEEYVQLSEFPGYEEFANQEELMDTW
                     TAFMDFRDNVLKALEEARNSKLIGKSLEAKLTVYPKQQVREMLKALDADIAQLLIVSP
                     DYFEVMAADEQVPDNAMVFDDVAITVEKADGETCDRCRQVRKDVGVDEKLPHLCGRCA
                     KIVEDFYPEAVAEGFEEK"
     gene            complement(44132..45658)
                     /locus_tag="IWO89_000142"
     CDS             complement(44132..45658)
                     /locus_tag="IWO89_000142"
                     /EC_number="1.1.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate dehydrogenase"
                     /protein_id="HAQ0454933.1"
                     /translation="MSNEKNVLFTIFGGTGDLAQRKLYPSLFRLYRKGNLGEHFAVIG
                     TARRPWSDEHYREVVKETIRALNPSDEEATTFASHFYYQSHDVNDSTHYQTLKELSDR
                     LDGHYQLNGNRVYYLAMAPQFFGTIVSHLKSQNIMTKNGFNRLIIEKPFGSDYQSAFE
                     LNQQIREVFPEQDIFRIDHYLGKEMIQNISAIRFANNIFEAQWNNRYIDNIQITFGEN
                     LGVEDRGGYYDHSGALKDMVQNHILQVVALLAMEPPVAFSEKEIRTEKIKALKAIRIY
                     NEEEVLENFVRGQYAQGELDGVQFKGYREEDKVDAQSETETFVAGKFTIDNFRWSGVP
                     FYVRTGKRLTEKGTRINIVFKQVPVNVFKTSVDEPCDDTTLPPNVLTIYIQPTEGFSL
                     SLNGKEVGQGFETEPIKLEFRNSAEMVENSPEAYEKLLLDALNGDGTNFSHWEEVAQS
                     WHIVDVIRKAWDQTQPDFPNYKAGSMGPKAAFELLEKDGFEWIWQPDNWYRERGKLND
                     "
     gene            complement(45673..46320)
                     /locus_tag="IWO89_000143"
     CDS             complement(45673..46320)
                     /locus_tag="IWO89_000143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005881023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent transcriptional regulator"
                     /protein_id="HAQ0454934.1"
                     /translation="MTPNREDYLKIILELGGDTTKVNNKQIVSSLDVSAASVSEMISK
                     LVKEELVEHSPYQGVQLTASGLQKASSLIRKHRLWEVFLVEHLDYSWNEVHDDAEVLE
                     HVTSEHLADHLENYLNHPEHCPHGGSIPKKEEVVHEERRQTLESYPVGTKVRIARVLD
                     EKELLDYLVTIDLNINEEYEIVDIAAYEGPITIQNDRKKIPVSFKAASTIFVDKI"
     gene            complement(46504..46833)
                     /locus_tag="IWO89_000144"
     CDS             complement(46504..46833)
                     /locus_tag="IWO89_000144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4828 domain-containing protein"
                     /protein_id="HAQ0454935.1"
                     /translation="MIYFLGSLITVLSSFIYLKKKKQAEKSEGLSKYQGTWFFSDSQQ
                     KQQLQITSDYTFLINGQSLGTSVVEIDEYRLVVRDQYGFYLIFSYPEQTLYDESADTS
                     YKLLSEK"
     gene            47010..47738
                     /gene="sfsA"
                     /locus_tag="IWO89_000145"
     CDS             47010..47738
                     /gene="sfsA"
                     /locus_tag="IWO89_000145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA/RNA nuclease SfsA"
                     /protein_id="HAQ0454936.1"
                     /translation="MKYQQVEIAFFIDRPNRFIAHCMKKDGEIIIAHVKNTGRGKEVL
                     IPGAEVAIVFAPGPKRKTAYDLIAVKKKGDWFNIDSQLPNRLAIDGILDGTILLPGLT
                     GPFTVYKREVTYLHSKFDIYLETVVGQKAFVEVKGMTLENKAIGAFPDAPTLRGLKHI
                     GELTYAAQDGYAAYVLFIAQFEHLHLATIHEEMQPALADMVRHAQQSGVQILAYNCQV
                     SADEVTVKQAIPFDVDIPFEDPIQ"
     gene            47754..48767
                     /locus_tag="IWO89_000146"
     CDS             47754..48767
                     /locus_tag="IWO89_000146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HAQ0454937.1"
                     /translation="MINLGIIGTNWITHQFVQAALSTKKYELTAVYSRHLEKAQEFGS
                     HYEGDIEFATDLKTFFNITHMDTIYIASPNSLHFSQAKQAILAGKNVIVEKPAFSTPK
                     EMDEIIHLANEHNVYFFEAARNIHESGFKKIADYLPLKDEIIGANFSYMKYSSRYDQV
                     LDGEEPNIFSTHFSGGALMDLGVYLVYAAVAWFGMPKEVHYFPRKISTGVDGLGWGIL
                     RYENFDVSIQPGKIGDSYLPSEIYFDSGTLVMNGVNAIEQAQFHDRAHKEIIDLAVTA
                     AENPMADEAEDFANVMAHPKDPAWGLLYEEWIELARNVNQVIYDLRKNGGIKFDADNE
                     EDF"
     gene            48767..50044
                     /locus_tag="IWO89_000147"
     CDS             48767..50044
                     /locus_tag="IWO89_000147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HAQ0454938.1"
                     /translation="MNEFVQVFPENWQKKYEDKGFISPSLIQRAVFQPLSNKQSIVAV
                     SPTGSGKTLAYLWPLLLNVEPGEASALVIFASSQELAIQVADVAREWGKDKELKVQSL
                     VGGANVKRQIEGLKEKPEILIGTPGRILELMKAKKIKAHQVKTMVFDEADQLFDAGNS
                     QIIDQILHQAPTEYQLAFFSATADRSLESIEKITGKTFETIDVTAEDDSRKGLRHYFL
                     RVPIRKKDDYLRRLTHIEDFHGLIFFNQLTELGVMEEKLLYRGVPVGSLASDQNKLLR
                     KAALEQFKKKMVRALLTTDVAARGLDITGLPYVVNAEVPLSEEAYLHRSGRTGRMGNE
                     GIVITLVQDNNLRDLKRLLRPSNITLEEIFLYGGKLQTEQPTKEDAAPAVNKKYAKTF
                     SGKEQNPEYNKEKIRKKVKNKSKKERNKGARRK"
     gene            50107..52809
                     /locus_tag="IWO89_000148"
     CDS             50107..52809
                     /locus_tag="IWO89_000148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAQ0454939.1"
                     /translation="MKERQEEILQLLTENQQGLTASDVAERLTIDRSNASRYLSELYK
                     AHHIVKTAGRPVVYSLPTEKSKSDEVHVDSSTQVTFETLVGENDSLKVSIQQAKAAIL
                     YPPRGLHTIIFGETGTGKSMFAECMYHFAIDSEMLSADAPFVSFNCADYAQNPQLLFG
                     HIFGIKKGAYTGAAQDSPGLIAKADGGILFLDEIHRLPPEGQEMLFTFIDKGIYRPLG
                     ESAQVHEASVQIIGATTESSESFLSTFNRRIPMAITLPNLASRSLDERYEIISLFIKQ
                     EANRLNQRIDIEKEAILAFMLYDAEGNIGQVKRDLKLVCAKSFLHYRTHHEKKLIIRK
                     EELSLQVQKGLLKIKEIPERLDRFMDSKSQYLTFEPGFADVVWSQDPERNMQVYNDIE
                     EKVTSLSETGVENIDLETLISKDVDAYFQTYVKELTKSSIPKDLLPDEIWRLTNQLYD
                     VAEKRLDRIYNEKARFAFALHLQSTLDRVREGHMIVHPDLNNVRKNFKREFQVAIDLS
                     TIIEEEEQVEIPFDEIGFISMFLSINLGESEPVKENKVDVLILMHGRQTASSMLEMAQ
                     ELLGTKVGKAFNMPLVMEVQTMYEEVLQYVKNYQEHLTNGLLLLTDMGSLNTFSDLIY
                     EETGIRTKAITMTSTMIVIEAIRMASVGRSLEDIYQNIQLSFESIVRDQFKAEKSETL
                     QRKKAVIVTCFTGEGVAAKLYQRIMPVINQNKVELIQMQFLERESFKKHIDALLEEYE
                     IKAIAGTVDVDYQNIPFFSAYDVFDDEKLNILKRIADDEIPTAKIVDSLAGSLSDVFS
                     IDELIRSLQKTVQQIQTDLSIIVEPGVDTGIVIHLAFMLDGLIKQESHRQFEKLSIFS
                     KIYRLEMDIVRANLMALERKYQVKIPEDDIAFLTQMFLENKIKK"
     gene            52961..53278
                     /locus_tag="IWO89_000149"
     CDS             52961..53278
                     /locus_tag="IWO89_000149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAQ0454940.1"
                     /translation="MAKKTIMLVCSAGMSTSLLVTKMQKAAEEKGMEADIFAVSASDA
                     DNNLESKNVDVLLLGPQVRFMKAQFEQKLAPKGIPLDVINMQDYGMMNGEKVLAQAEN
                     LMK"
     gene            53308..53628
                     /locus_tag="IWO89_000150"
     CDS             53308..53628
                     /locus_tag="IWO89_000150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HAQ0454941.1"
                     /translation="MEDQQNLEAIMGLIMFGGNAKSDAMEAIAAAKIGDFELADRKIA
                     DAEESLIQAHHAQTDMLTQEAQGNHMQVTLLTVHSQDHLMTSIAFTDLAKEIIELYRR
                     VYSE"
     gene            53736..55196
                     /locus_tag="IWO89_000151"
     CDS             53736..55196
                     /locus_tag="IWO89_000151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAQ0454942.1"
                     /translation="MNGLSSWMEKYILPVAAKIGAQKHLVALRDAFIGTLPATMAGSV
                     AVMINAIIRDLPQQFNSTYADSLAADKGIFAVLNVIIGINGFVWNGTLAIAGLIFAFS
                     WGYNLARAYGVNDLAGGIVGLATLIQGISFGPALSAALDVNVPSNVADAINSSEIGAT
                     IADGTLSVGLWGWLKLDHLNGNAYFTVMIMGAISVIIFCKLMLANITIKMPDSVPPAV
                     SKAFAAILPATIALYVIAIINYIVGRVADGQLIIDLVQKYIAEPFLGLSQGLGAVLIV
                     TVFVQVFWFFGIHGPNVLAPVLEGIWGQAQLINIDIFQKGYNGKTGTPAVLDAIEDGK
                     AYMWVRGSFDAFAWFGGSGGTIVLLIAILIFSKRADYLTVAKLSIGPGIFNINEPVMF
                     GLPIVLNAIMFIPFLIAPVVATAIGWTATYLGLVAPVSQQVTWVVPPFLLSFLATGAD
                     WRAPIVTLVCMVVTFLIWAPFVISANKMDPAAGEQQ"
     gene            55264..55485
                     /locus_tag="IWO89_000152"
     CDS             55264..55485
                     /locus_tag="IWO89_000152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0454943.1"
                     /translation="MITIASAMQQDEIKELLEGYNEEGMTFTFKEKQGIKLFFETSAE
                     DPEVAAKKAKELIKAQPWGTVLYFQSTAV"
     gene            55516..55698
                     /locus_tag="IWO89_000153"
     CDS             55516..55698
                     /locus_tag="IWO89_000153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3188 domain-containing protein"
                     /protein_id="HAQ0454944.1"
                     /translation="MIKNGLFLCSIGSLIVLYSLNPHSQRYDLYSMVTGLFLTGLGIY
                     FFMKGKKREEAKEKNN"
     gene            55698..56111
                     /locus_tag="IWO89_000154"
     CDS             55698..56111
                     /locus_tag="IWO89_000154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3284 domain-containing protein"
                     /protein_id="HAQ0454945.1"
                     /translation="MEITKKLNIPASFFYDKVMDSVLFDVRKATGKSVTRKQLKNLEY
                     VKQFSQNNRARIKIEEVIENQSYKFRTSTTKNEFVVHYQIKPLDDSTCEVHYTEKMES
                     YGMLQKLNDMLLGTILMYFKRRRFKKMLDMIEATY"
     gene            56234..57415
                     /locus_tag="IWO89_000155"
     CDS             56234..57415
                     /locus_tag="IWO89_000155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAQ0454946.1"
                     /translation="MTNKVRELGVSSIEEMFDLATYAFNGADTPERRERFRTLAENSW
                     NYGFFDEEDRLTSQVMATPFPVNFYGQEYLMAGIGYVASYPEARGQGGINQIMEKILE
                     DCRDRKVSLSYLAPFSYPFYRRYGYEQSFDKISYQLNSRDVPFIKKKSGKIKRMDFED
                     AKAAIRRIYEQMPENQRAGLKREDWWYTYKFKQKGNYQFALHFDDNEEATGYIVYQLA
                     TSSFIIAEWGFLDHDAFCSLVRFVSSHNGAFETFEYSCGYGGNDQNYLLENPFASVMI
                     TPYMMARIVDISLFLENYPFKKRQAAFALQIEEDHYAKWNEGIVEVTIENGKNHLSKV
                     EQTKLPIVRGSIQHITQLLLGYRKVEELVFQERIQVTKEYEETLSSILPQQQPILNDY
                     F"
     gene            57804..57877
                     /locus_tag="IWO89_000156"
     tRNA            57804..57877
                     /locus_tag="IWO89_000156"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:57838..57840,aa:Arg,seq:cct)
     gene            complement(57914..58114)
                     /locus_tag="IWO89_000157"
     CDS             complement(57914..58114)
                     /locus_tag="IWO89_000157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HAQ0454947.1"
                     /translation="MKINYTVDLVSFSLVINFSLFSLRLFIDGLRHTHASILLYQGVN
                     ILSVSKRLGHSSLETTMSTYTK"
     gene            complement(58419..59651)
                     /locus_tag="IWO89_000158"
     CDS             complement(58419..59651)
                     /locus_tag="IWO89_000158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HAQ0454948.1"
                     /translation="MLSDFNELLKKYARLIAETGVATEKGHTVVLQISVEQAPLARLI
                     TQEAYKLGAAEVIVQWTDDQIQREFLLHAATDRIENVPQSKIDQADEWLEKGASRISV
                     VSADPDAFAGVDSHRVASYQSANGKALMNLRKATQANKVSWTVVAAAGKQWAAKVFPE
                     LPEEKQVEALWDQIFKTTRVYEEDPVLAWKKHDEKLAKKAEELNREQFSALHYTAPGT
                     DIIIGLPKNHLWEGAGSYNARGEKFMANMPTEEVFTAPDSHRVDGYISSTKPLSYAGT
                     IISGMKFTFKDGKVVDFSAEQGEDVLAKLLDTDEGARRLGEVALVPDPSPISQSGIIF
                     FNTLFDENASNHLALGSAYAFSVKGGTEMSDEELAEAGLNRSQTHVDFMVGSDKMDID
                     GIREDGSTVPIFRNGDWA"
     gene            59905..60474
                     /locus_tag="IWO89_000159"
     CDS             59905..60474
                     /locus_tag="IWO89_000159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HAQ0454949.1"
                     /translation="MARKKTITREQILAAAYEVAATDGFTRFTARNIANKMNCSTQPI
                     YLEFKNMDDLRQALFEQIYDHLQYEVFTVEHTGNTIIDLAFNYIHFARREKKLYRSLY
                     LEEYGGGKEMQNFSYTYFVNTVKRDPAYVNLSEEQIESLYNGTWIIATGIAALMSSSI
                     IHPTDEQIEKMIQDSIDAILKFKEPIEID"
     gene            complement(60652..61092)
                     /locus_tag="IWO89_000160"
     CDS             complement(60652..61092)
                     /locus_tag="IWO89_000160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin"
                     /protein_id="HAQ0454950.1"
                     /translation="MALAKIVYASMTGNTEEIADIVAEAFENLDIEVEINECTQVDAD
                     EFEEADICVVATYTYDDGDLPDEIVDFYEDLQELDLLGKIYGVCGSGDTFYDEFCKSV
                     DDFDAAFAKTGASKGAENVKVDLNAEEEDIENLEAFAKELVAKI"
     gene            61250..62014
                     /gene="map"
                     /locus_tag="IWO89_000161"
     CDS             61250..62014
                     /gene="map"
                     /locus_tag="IWO89_000161"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008377375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HAQ0454951.1"
                     /translation="MITLKSAREIEMMAESGALLADVHKNLRDFIKPGITSWDIEVFV
                     RNYIESHGGIAAQIGFEGYEYATCISINDEICHGFPRKKPLKNGDLVKVDMCIDLKGG
                     ISDSCWAYVVGESTPEIDRLMEVTKKALYIGIEQAQVGNRIGDIGHAIQTYVEGENLA
                     IVRDFIGHGVGPTIHEEPAVPHYGEAGKGLRLKEGMVITIEPMVNTGTWKMKMDPNGW
                     TAYTRDGGLSCQYEHTLAITKDGPRILTSQGEEGTY"
     gene            62046..62969
                     /locus_tag="IWO89_000162"
     CDS             62046..62969
                     /locus_tag="IWO89_000162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihY/virulence factor BrkB family protein"
                     /protein_id="HAQ0454952.1"
                     /translation="MKVWNKIKQNENLMRFIETTQKRITDSEMSTTSVVVAYYLLLSL
                     FPLIIAFGNILPFLHIDQETVLTYLREVIPETVYQFIGPAIKDLLTQSSGGLLSVSAL
                     AALWSASQSINALQIAMNKAYGVENRKNFLIVRFFSLIVIVLFMIAISGVTIVLGLGQ
                     LILDAIQPIVQIPVNFIDQFQTLKWPITLVALFVIMFLIYLIVPNAQLKLKTIIPGTI
                     FATVGWMLLSQVFSIYARYFATRISGYQIIGSFIVLMLWLNFAATIIILGGIINAVVQ
                     EYVTGHQIKKRRDPSRKWFNKLKTKFSHKKK"
     gene            63045..64184
                     /locus_tag="IWO89_000163"
     CDS             63045..64184
                     /locus_tag="IWO89_000163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl/decaprenyl-phosphate
                     alpha-N-acetylglucosaminyl 1-phosphate transferase"
                     /protein_id="HAQ0454953.1"
                     /translation="MLVSFSMVIRFFLTILLSLILTPVFKIISVQTGMVDKPNERRIN
                     KVPMPSAGGLPIFISFVISTLFLFRKIIPQSYIVPILIASLVIIVTGVLDDKYELTPK
                     QKSIGILIASLIIYFVADIRIDSFTLPFIGYIQLGWLSFPVTIFWIFGITNAVNLIDG
                     LDGLAAGISLIGLITIGIIGYFFLHASTVYIPVVIFCLVASIIGFFPYNFYPAKIYLG
                     DTGALFLGFMMAVLSLQGLKNVTFVSSISLLIVMGVPVTDTFFAIIRRKANRVSFSTA
                     DKKHLHHRLISLGFTHKGAVLTIYAMALMFSFTAMVMNYTGRIGTIVLIIAMLFAAIL
                     LFELIGLINEKHQFILKTLRFLGNKEYRTQVKQKYLKKWNRHIDE"
     gene            64177..64977
                     /locus_tag="IWO89_000164"
     CDS             64177..64977
                     /locus_tag="IWO89_000164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HAQ0454954.1"
                     /translation="MNNKLNQPILISIVTANSDKIFRTLDNFISTVKDETAVKIRIFD
                     NNSLPEYKKRLTEYRKYPYIDIYFHDENSGFGYGHNHNLIASDSRYGIICNPDILVDE
                     ETVTALVLLLKEHPECAMLAPKILNEDGTTQHLIREQLAVFDYVLRFVPFKWVKKKFD
                     KRLAVFECRGLPTDANSYVRMISGSFMVADIDKFKEIKGFDERFFMYFEDNDLCLSFE
                     KAGYKLLYTPFYAVIHFYGKGAHRNAKLFRVFLTSMMKFFNKWGWRFF"
     gene            64977..65804
                     /locus_tag="IWO89_000165"
     CDS             64977..65804
                     /locus_tag="IWO89_000165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HAQ0454955.1"
                     /translation="MKPEILVVVVLYQQLFSQSPAYGPLSKALSEKQIQLIIYDNSPQ
                     RQQNELFEKENIVYHHDPQNPGLAVAYNFALSQADEGTRCLVTLDQDTRLVDDYFELL
                     RKVTFTDECVAAVPMIFSGSRQVSPLYSDRYINRTAQAVEVEGPTAERIMAINSGVAW
                     SVKFLKKIGGFNPAFSLDFLDHWLFWKVDQLEKTVEILPVRMEHDLSVLDYEKVSSNR
                     YRSILQAENRFYHEYDQRHLSSHRRQLLLRTAKQFLTVKNRHIWRMTLRSYVKNWKV"
     gene            65782..66516
                     /locus_tag="IWO89_000166"
     CDS             65782..66516
                     /locus_tag="IWO89_000166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HAQ0454956.1"
                     /translation="MLRIGRCEFVRSVCIATFNGGRYLREQLDSILSQLSAEDEVVIS
                     DDGSVDDTWDILTEYAEKDKRIQLFSGPRQGLIANFGYAIQQTKGDLIFLADQDDVWL
                     ETKVKKITDYFEQNPDQLVVVSDLIIVDSELQEIHDSYFSFRNSKEGWLNNVIRSHYI
                     GAGMAFRSSLKPDILPIPSEVPMHDMWIGLLGGKKTGFLREPLTLYRRHDLNASEIET
                     HSKLRQKISWRVHLITALIKRKWFKR"
     gene            66616..67482
                     /gene="rfbA"
                     /locus_tag="IWO89_000167"
     CDS             66616..67482
                     /gene="rfbA"
                     /locus_tag="IWO89_000167"
                     /EC_number="2.7.7.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase RfbA"
                     /protein_id="HAQ0454957.1"
                     /translation="MKGIILAGGSGTRLYPLTKATSKQLMPIYDKPMIYYPMSTLMLA
                     GIKEILIISTPQDTPRFKELFGNGNDLGLQIEYAVQESPDGLAQAFIIGEEFIGDDSV
                     CLVLGDNIYYGGGLSKMLQRAASKDTGATVFGYHVNDPERFGVVEFDENMQALSIEEK
                     PAQPKSNYAVTGLYFYDNEVISIAKGIKPSERGELEITDVNKAYLDKGKLSVELMGRG
                     FAWLDTGTHESLLEASTFIETIEKRQNLKVACLEEIAYRMGYIDKEQLLALAQPLKKN
                     QYGQYLLNLAAE"
     gene            67496..68068
                     /gene="rfbC"
                     /locus_tag="IWO89_000168"
     CDS             67496..68068
                     /gene="rfbC"
                     /locus_tag="IWO89_000168"
                     /EC_number="5.1.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010771301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose 3,5-epimerase"
                     /protein_id="HAQ0454958.1"
                     /translation="MKVTDTKLTDVKIVEMDVFGDHRGFFTESYSKAKFAEHGLKYDF
                     VQDNHSLSAEAGVLRGLHFQKGEAAQTKLIRVVTGAVLDVVVDIRKGSPTYGQWDGFI
                     LSEHNHRQLLVPKGYAHGFVTLTPNVNFLYKCDNYYNAEADGGIAFDDPDLAIDWPID
                     PSRAIMSEKDHRHPTLKEFEAENPFVYGEI"
     gene            68090..69118
                     /gene="rfbB"
                     /locus_tag="IWO89_000169"
     CDS             68090..69118
                     /gene="rfbB"
                     /locus_tag="IWO89_000169"
                     /EC_number="4.2.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="HAQ0454959.1"
                     /translation="MKNIIVTGGAGFIGSNFVHYVVNNHPEVHVTVLDKLTYAGNKEN
                     LAGLPSDRVELVVGDVADAELVDRLVKEADAVVHYAAESHNDNSLKDPFPFVQTNIIG
                     TYTLLEACRKYDVRYHHVSTDEVYGDLPLREDLPGHGEGEGEKFTAETPYNPSSPYSS
                     TKAGSDLLVKAWVRSFGLRATISNCSNNYGPYQHIEKFIPRQVTSILSGIRPKLYGEG
                     KNVRDWIHTNDHSSAVWLILTKGRIGETYLIGADGEEDNKTVMELILEMMGQPKDAYD
                     HVNDRAGHDLRYAIDSTKLREELGWQPEFTNFRDGLADTIKWYEEHEDWWKKEKEAVE
                     AAYAKNGQ"
     gene            69216..70067
                     /gene="rfbD"
                     /locus_tag="IWO89_000170"
     CDS             69216..70067
                     /gene="rfbD"
                     /locus_tag="IWO89_000170"
                     /EC_number="1.1.1.133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose reductase"
                     /protein_id="HAQ0454960.1"
                     /translation="MFLLTGGNGQLGTELRHLLDEKNLPYVSTDAKDLDITDAEKTMA
                     YITELKPDVIFHCAAYTAVDKAEEEAKELDEKINVDGTRNVALAAKAVNAQFVYISTD
                     YVFDGKNKNVEYEVDDQTNPLNEYGRTKLLGEKAVEEILEDYYIIRTSWVFGIYGHNF
                     VFTMQRLAETRDKLTVVNDQFGRPTWTRTLAEFMVYVIEEKAPFGIYHLSNENSCSWY
                     EFAKEILKDTDVEVAPVTSEEFPQKATRPQYSVMSLKKTEALGFVIPTWQEALAQMLE
                     AAKKMNK"
     gene            70102..72135
                     /locus_tag="IWO89_000171"
     CDS             70102..72135
                     /locus_tag="IWO89_000171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0454961.1"
                     /translation="MEKQTRSARRKEKPKANQADSNLVLDLFLKLRYLIGVLVIVVIV
                     TFNLNGSSLGVWDKYVSQRDDGKKTDVIFGQNREVRSDEWLVQTPFYLSQAEEDFSLV
                     NEDYSLNGQNMIIAYNSPVKDITVIGKPFNWGFFFLGRDRGLSFYWAMKLVVMVLLGF
                     EVLMILTKRKKALSLIGAFALTFSPAVQWWFMQHVGDLIFFTLGLMIAFYHYFYNHDK
                     KWLRTLMMLLVVIFGIGFILVIYPAHQVMLAYLLIFYFVGLLIYYSKKVSWDLLDVVL
                     IGGAVLFIGGVMVHFWLTSKDALLASLNTLYPGKRVSTGGKWTIGEFFSFLTNWKIPF
                     EDINYSNNSEVALFYHFFPTVFLASPFVLFGKKDSEQKLLGRILMIFCLFSIFWITVG
                     LPKWLAEITLLSYVPPLRAILTFSFAACLLSIWFIAYIWREDVIPIWYKFILLAVNAW
                     SYFYAITHSKMDNYFSDFETIAVAVLGTVILAFALFGWRRLFYLAFVALVIVSGFFVN
                     PVVEGTGAIFEKTLAKEIQKIDQQDPGKVWLSEDELYNFTPTLGVKSFNTVRFYPDIA
                     AWKKIDPKEEYEKYYNRYAHTRAFIAKEDTKFILDRPDMFSVTLNFEDAEKLGIDYVI
                     SKRPLTDYNQLYEAQFTQLYGPDKDGYMIFKVEYPDYPDVPQTQTQENQLVVQ"
     gene            72179..73459
                     /locus_tag="IWO89_000172"
     CDS             72179..73459
                     /locus_tag="IWO89_000172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EpaQ family protein"
                     /protein_id="HAQ0454962.1"
                     /translation="MKIIQAIVQKFVNKMSVGFLLLLLTLVFFVWTAGVGFPATLFIY
                     NNSSLLIAFSVAILLILNVYKAKGVDFLYLGVALALFIFFSYFRELRNESLFGDLLFP
                     LVILFCLVIKWIEWTKWDRGIYLIVFTVFLGITLYRVFTEIQVPEGQSIWAPGNSLSD
                     IWINTNTIGSSLMTLALLISGFASSFERWYVRIIGGIAVVAAFLGIWVCQSRGALVAL
                     IVFAILDLLPKSLMRVIRAPFIAYTVTILLALPISYLAAVSEKVNLFTDREDIWHKFY
                     QTLGEKSEQVLLGMKTFIFQRGNQFLGNHNSYNSILNIYGLIGFGIAALLLILFIGRL
                     TLKADLSNGQMTFIWAFFAVMMQSFMEDTLTSYPWVPIIYILLAMAVHRYDEPEVAEE
                     FYVMEEAYDDMTYDDTSYEEQPDSAHSSRIQRYH"
     gene            73669..74475
                     /locus_tag="IWO89_000173"
     CDS             73669..74475
                     /locus_tag="IWO89_000173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAQ0454963.1"
                     /translation="MFKDLYLFMKDIYQNRKLLLQFSINDFKSRYAGSFLGILWAFIN
                     PLFTVLIYWLVFGVGLKAQMIDAKYPFIVYLVTGMIPWFFFSDAFASTTVVFREYTYL
                     VKKVVFNIRILPTTKILSNLYTHLFFILIGIAVALINGIYPSAMSLQLIYYLFCMCAF
                     LTGLTWLTASIQPFLPDIMQLITIVLQLIMWTLPILWSPNQFDSGLVSILKLNPLYYV
                     VQGYRESFLSEAWFWEHGTYTLYFWVFTLVMFVIGSAVFRRLKPHFSDVL"
     gene            74487..75707
                     /locus_tag="IWO89_000174"
     CDS             74487..75707
                     /locus_tag="IWO89_000174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ0454964.1"
                     /translation="MTEYAIRLKDITKSYNMYAKPSDRFREALNPFKKSYHDVFYALK
                     NVNVEIKKGEMIGFVGENGSGKSTMLKIITGVLTPTSGTMEIEGKISALLELGSGFNP
                     EYSGYENIYLNGMVLGFSREEVDEMVDDIIEFADIGDHIYQPVKTYSSGMFVRLAFAV
                     AINVNPDILIVDEALAVGDLEFQLKCMEKFTEIKNSGKTILFVSHDISSIRRFCDRSY
                     WLKNGEVVEYGDTMEVTTNYENFLKKKSVKTVDRNKNQVERFAAPDIVDVTKAELLDK
                     DKNPVDMLEQGEKLYVKVTYEVKDDTIKNPVLGIALRTVDNFYVCGLNTLLDGIKIPW
                     KKGENIFYLEYEKLGLLAGEYYFDVAVFEENATVPLVYKTKYMNLFVSGSYIGEGIVV
                     LDHKWEEGTHSNEI"
     gene            75697..77283
                     /locus_tag="IWO89_000175"
     CDS             75697..77283
                     /locus_tag="IWO89_000175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HAQ0454965.1"
                     /translation="MKYDFEMDLDEQSSVGKIAAQIKPGSKVLEFGPGNGRLTKHLIG
                     AKQCEVSIVELDKELFDFVSEFAQDGFYGDIESFEWANYYAGQTFDYVLFADVLEHLV
                     DPGKTLKKVREFLNEEGEILITFPNLAHNSVMIDLFNNQLPWASYGLLDETHNSFYTH
                     DGFQKVFEKAGLFINIEDYLYLAVGDTELKSTYEELPEAVRYDFKMRPFGEVYQYFFS
                     LMKHPVAQSSIAEPQNSNYVKVLEVTQQTKQDETIQQIPFNNFTGENETLSFPVSETT
                     ERMVFRFAQQPSFIEFSAEAAGEKLTFIDSNAVVKTVNDCYLFDGKELPEFVLTDISG
                     KEVTIHCHYRFIGELTPTMKELLETIRPMAEVTKQLSEKNDELERENHHLLEENTRLD
                     HTLKTTTNRYCTLLESDEWTIKHRLGRRKKETSKKIQEKELSICIDEKIWDAETKILK
                     IIGWGIANSDRQPLSYKLSADQSPFFEAIPVSREEVNQAEQLAKGTEAGFELRILCEQ
                     ERSFLVEAVAQNGQSWIIEM"
     gene            77322..79460
                     /locus_tag="IWO89_000176"
     CDS             77322..79460
                     /locus_tag="IWO89_000176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HAQ0454966.1"
                     /translation="MQKKEIAVFIDQITRERATGDLLIVGWAIDEVTKEIPTIKVEKE
                     NVIAEATHVVRLDINHLYNLDVKTQSGFKVRLSGKMRGKAILDFQTARHQNGIAVKLN
                     GKYPYDDGIESSWERKKRLLKKGINYARTHGVKKAIRRVKLELKPGSIDYAEWISRHE
                     KPDLKEQRKEAQGFAYRPLISILMPVYNVEIKWLEKCIDSVLDQTYDHWELCISDDAS
                     TDPAIRKCLESYQAKDDRIKVVFRKENGHISLATNSALEIAEGEFIALLDNDDELPPF
                     ALYEVAKVLNVHPELDVIYSDEDKIDADGNRFDPHFKADWSPDTLMGNNYISHLGVYR
                     TSIVKELGGFRKGYEGSQDYDLVLRVTEQIPADHIYHIDRVLYHWRTIPGSTASNGEA
                     KSYIYDSGVKALTDALSRRNIKGSVRPGRISGFYEIAYDVLQEDLVSVIIPTKNGYED
                     LKTCVDSIIEKTSYSNYEIIIADNGSTDPKMQELFAEYKHQLKDRFIVELIDIPFNYS
                     RINNLAAEKANGKYFLFLNNDTEVIEPDWMTVMVSYAQFDRIGCVGAKLFYPDDTTQH
                     AGVLLGIGGVAGHALNNYDRTHCGYFGRLVIDVNYLAVTAACMMVKAADFNAVNGFDE
                     TLEVAFNDVDLCLKVYELGRYNVYAHQAELYHFESKSRGYEDTPEKQRRFAGEIKKMQ
                     DKWPAYIAHDPFYNDNLTKDGIGDFSLRPD"
     gene            complement(79828..80811)
                     /locus_tag="IWO89_000177"
     CDS             complement(79828..80811)
                     /locus_tag="IWO89_000177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HAQ0454967.1"
                     /translation="MNKQELHDFIGFRQPNICQIVAYKDGVEVYTDEWNGYKKDDTCH
                     IMSATKSIVSLLIGIALEQGLIKSIDDKVLDYFPEYKVKRGEKTIYAITIKHLLTMKA
                     PYKCKGDPWTKVCSSEDWTKTSLDLLGGRKGITGEFNYQTVCLHILTGLLYQTSKLTT
                     VDFANKYLFDPIGIPNHVNYYAETAEEHKQFTMDKSPKTNVWFCDPLNIGTAGYGLCL
                     SASDLAKIGVLCLNKGMYKGKQIVPPMWINEMFKPRTSALEDLQNMRYGYLWWVLDSE
                     NEIYSAIGNSGNVLYIDAKNNLVISITSYFKPMIIDRVAFVEEYLKPFILK"
     gene            81189..82580
                     /locus_tag="IWO89_000178"
     CDS             81189..82580
                     /locus_tag="IWO89_000178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HAQ0454968.1"
                     /translation="MNKNGEWSAARRIFIILMDVIVYNAAIYSSFLLKFRGEIPLRNL
                     ETFQHSAIIISVIFIALNILLGAYVFYNRMISDIVFVTVIGQVLMTLGIMVVTFAGRW
                     FAFPRLVILLSFVLGTLFLSIYRILVYKLYLRVSRDKKVVIIGLEKDVAPAIQNFGSK
                     KNNKHKVEAVILDNYYANIKNMIKDIDIVYLASHIEETEKIKIYQLVTKNEKKLFLNT
                     TFENLTLINPNIMNFEDESIIEASGFRIQPEYELFKRLFDIIVSLVLLVIASPFMLFT
                     AILVKVTSPGPIIYKQVRITKNQKEFSIYKFRSMTATAEAKSGPVLAKSNDARVTPVG
                     KFIRAVRFDELPQIFNVLKGDMSIVGPRPERSFFVDQFNAENPYYYLRHNVRAGITGY
                     AQVYGKYVSDYNSKLRFDLLYIKKYSLMLDLKILLQTIKILFDKMSSQGLEEDENVIS
                     LAEIAFPNEKIFR"
     gene            82596..83636
                     /locus_tag="IWO89_000179"
     CDS             82596..83636
                     /locus_tag="IWO89_000179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HAQ0454969.1"
                     /translation="MNNPKFSIIVPVYNAEATIGRTIDILKELDYEEYEVVLVNDGST
                     DQTQEIIQELIENDERFELITTTNQGPGLARNEGIRQSKGEYLLFFDADDTPVKTILS
                     DYDHLLEKSPKADLVVSSFVFRTMDEGRIVSEKENLVTECHYLTHEEFIKDMYQLMNE
                     QLMYVVWNKCYRSDILKNNQILFKTYNSCEDRIFNLHYYEYCHQVIMNPKVEYIYEFE
                     GGKGITNQYRPNKFETFKEFYSLANTVTDDINKDGMAALLLKGTTSVIFSIYSTSART
                     SKEKKDEAKQILSDPVIREAKQIAKTDSSAKKITKLLYNMPAPLFLSAVKVGSFVEVK
                     MPGLMAALKRVY"
     gene            83655..84740
                     /locus_tag="IWO89_000180"
     CDS             83655..84740
                     /locus_tag="IWO89_000180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 1 protein"
                     /protein_id="HAQ0454970.1"
                     /translation="MKRILHFQGRMGLGGAESFMMNLYRKIDRTNYQFDFLIYEDYAD
                     VQDYHSEIERLGGRIFVVPNPKKNIFKYLIEVNKLLKKESFSIVHNQVYFGGGINLWL
                     AKKNGIRQRIAHSHATEDGKSQNIVMNVLRKFLTKLLLQNATDYLAVSQEAGESLFQN
                     HPFEIVHNGIDLELYSKNSEAKVNKRKELDISMSTFVVGNIGRLEKQKNQSYLLEIFL
                     EILKMNSDSYLILVGEGSLRNKLKAKAVELDIEKRVKFLGERNDIPELLSVMDVFVMP
                     SLYEGLPMVGLEAQAAKLKLVLADTISKDTKLTNNVDFVSLDETPQEWAKKVIKEPYA
                     NKITQELKSYDVSYTTEQMESIYSRDH"
     gene            84773..85735
                     /locus_tag="IWO89_000181"
     CDS             84773..85735
                     /locus_tag="IWO89_000181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-1,6-N-acetylglucosaminyltransferase"
                     /protein_id="HAQ0454971.1"
                     /translation="MKICYLILAYNDIGNLKRLVNRLNENAVFIIHIDKKTDIEPFIE
                     AFKSYDNVEFLCNKNRVKIYWGGFSIVQAEINLVKRALQNEKYLKYVLLSGADYPIKD
                     NEYIYNYFKKNSSVEFIRGIDLDQIKHKEFYYKHIDVYQKHDYPRINRNNTTAFKIFR
                     AIINRCLRMIKLPPKIRHHKFDLYHGSQWWALSKECLTELIQMYEQNQQDYLNFKIGM
                     FAPDEKFFHTLFFNSSFKNKNVIGGPDLPLELKNIEETTLQTSKLANIHIIDPSMNKW
                     FDERDIDQIKESEKLFVRKVKSDYSDKLLNRIDKEILKVGNIDE"
     gene            85728..87155
                     /locus_tag="IWO89_000182"
     CDS             85728..87155
                     /locus_tag="IWO89_000182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HAQ0454972.1"
                     /translation="MNRIVPVLEKSLLSRDAFRVINLIICLLYFMPSTFNVTNIPMKI
                     AFAWGGIIVLYEVFIKKNFFKMRFSWLLIGAMISFILTIIINRENFLIPSIYNFGYLL
                     ITLIVIFPADFSQSEEEKKKKMVYFNNVFIIIIFLAALISIYQFIFLISYHVPTGTPG
                     LLARQGFIEHRLFGVYTSPNVGAMFGYTSIILSLISVLISKITKTIRVFYMMNFIVQI
                     FYITLSSSRGAQVVICSFLVLFLLLFANSKFRKSIREVLPVKIKWMVSILLVLLVYFS
                     FGTNYAKDFLATIPVSIDRKVEVIESNESDKTPLPEKEVILQHSSDNSEISAGRFTIW
                     KAALKVIRPTLLFGYGETDFYGNSDAKFLKKVDLDTTDVNELKRAHGNMHNGFLQVLV
                     SSGIIAFICIYAFYVLNIFSLLKTFFRSKVNYAFLGIVLIFILSIFIDEMVEAHVLFN
                     KRDVISIVFWYYLGSISYLYLKKEN"
     gene            87157..88227
                     /locus_tag="IWO89_000183"
     CDS             87157..88227
                     /locus_tag="IWO89_000183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0454973.1"
                     /translation="MKTAFIAWTPLHLINILNVYQNFYRESKGDLFIYSEFENARRYS
                     ERIKELGLFNDVVFVNHRKIGSSFKRKINLLTNHNQFFSDDLSVYDEIFIQGENYFTK
                     ILFSNVYKKNPDVKLNYIEDGLGTYLKKSIFSTSKNVQRVFKILNKNSIFKKKIDDYF
                     VYCPELLLYDVKNIKKIPKIDENSKLMKNIQSIFGIEEVFVGDKCIFFDQPLLSDGYG
                     IDETDIFAKVNSIFSGKKETLIKLHPRARTNRYGEKNILNSSLPFEVLLLENDFSSTL
                     LISPISTISFSPSMMFEKTINSIMLAKLILNEYTALSEDKKETLTNIVLFCEHYNRLS
                     EEKILLPENWAELRKLVEYGII"
     gene            88214..89635
                     /locus_tag="IWO89_000184"
     CDS             88214..89635
                     /locus_tag="IWO89_000184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HAQ0454974.1"
                     /translation="MGLFNTLSKSKLVKSSFWYTIGNFFIKGISFITIPIFVNITTQA
                     EYGLINNFTSFVSIFSILVGLSLNSAINNASFDYKDDINEFMSSVLSLATLSLIVFLL
                     IGNIYFLFSNAFFDMNQLVFNLMLLQSFASFVISYIQSYFTINFNHFKYLILSFISTF
                     LNIAISVALMQTLYFDDKYVGRVVGGTLSFVLIGLVIYLRIMIKGAHFFDKKQWRYAL
                     KISLPLIPHSLANIVLAQFDRIMINSYLGPAAGGIYSYISNIGIILSVIWMSTNNAWV
                     PWFYGEMSKNNEKKVKKVSNYYLVVFTLITMIVMVVSIDLAKFMAPKDYYSGLSLVVP
                     ITLGYFFQFLYSLPVNAEFYEKKTSFIAIGTVASAIVNILLNMIFIPRLGIIAAGYTT
                     VFTYFLLFIFHYNIAKKITNKQLFDTKKICIIIIFVSIFSLILHILINQMLIRYVLTS
                     LLIISLVIYFLKIKNRIIDDYNS"
     gene            89648..90655
                     /locus_tag="IWO89_000185"
     CDS             89648..90655
                     /locus_tag="IWO89_000185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HAQ0454975.1"
                     /translation="MFKGKTILVTGGTGSIGSEIVRQLLKYEPKAIRIYSRGEEKQFY
                     MQQELSNHTNLRFLIGDIRDSQRIDYACRDVDIIFHAAAMKHVPASEYNPMEAVKTNV
                     MGTQNVIDAAIKNNVKHFVGISTDKVVSPTNTMGATKLLSEKLIIAANQYKGKTETIF
                     SCVRFGNVMGSSGSVIPLFSKQIKNNKDITLTDVDMTRFMMSIPDAARLVLSAAMNSK
                     FGETYVLKMPAINILTLAQSLIKIYNDTHDEKYRGEIKVVGIRPGEKLYEELMTTDEI
                     ERSYENDKMYIIPPHYHATDELYKDYEKIKITAYSSDATELMSGQEFIRIAKENNVVT
                     W"
     gene            90667..91671
                     /gene="neuB"
                     /locus_tag="IWO89_000186"
     CDS             90667..91671
                     /gene="neuB"
                     /locus_tag="IWO89_000186"
                     /EC_number="2.5.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminate synthase"
                     /protein_id="HAQ0454976.1"
                     /translation="MKSEVYVIAEIGVNHNGSLDLALESIDKAKECGANAVKFQTFKT
                     NKLVSKKAPMAKYQKDNLKEDSSQFEMLKKLELSQSDFIRIKDYCIQNKIDFLSTPFD
                     EESADFLKQIGVQGFKIGSGDLTNLPFLRKIDMYGLPVLLSTGMADIHEVSEAVDCFI
                     DSPVTILHCTSNYPASSEDINLLAINTLKEMFGIPVGYSDHSLGYDVGICAVAMGAKV
                     IEKHFTLDKELPGPDHKASLDPQEFADFVKHIRNTEIFLGDGVKRAMPSELSTKEVSR
                     KSLVVTENLNVGDILTEKSLTIKRPGNGIAPKFLYQYIGKKVKRPISADSVLLEEDVE
                     "
     gene            91668..92816
                     /gene="neuC"
                     /locus_tag="IWO89_000187"
     CDS             91668..92816
                     /gene="neuC"
                     /locus_tag="IWO89_000187"
                     /EC_number="3.2.1.183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine 2-epimerase
                     (hydrolyzing)"
                     /protein_id="HAQ0454977.1"
                     /translation="MKKILIVSGTRADYGIYKPVAENLLQDNWDVSFVVSGMHLSHKY
                     GFTKELILADGFRISGEVQSLFLENNKGNMARSVALEINGFTNIFEQTSPDFVLLLGD
                     RGEMLAAAIACLYLGIKTAHIHGGEVSGTVDESIRHAITKIASVHFAATEKSKERLIK
                     MGEDSWRVFPVGAPRIDTILNNRLPSFRDIKEKYHLKFEKKKYVLQVFHPVVTELENI
                     EKQILALINSMEQQSQPIICILPNSDAGSEIIRKMYQKYLKTDIIYITNLEPEEYLTI
                     LKECKFMIGNSSSGIIEAASFKIPVINLGTRQNGREQSKNIINANLNENEIETALNKI
                     QDPQFIKSLKNVKNIYGDGNSSRRITKTLNQILNDGEDIKWIQKQIAY"
     gene            92789..93466
                     /locus_tag="IWO89_000188"
     CDS             92789..93466
                     /locus_tag="IWO89_000188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyltransferase"
                     /protein_id="HAQ0454978.1"
                     /translation="MDSKTNSLLKYTSDPYIIIGAGGHAKVIIETLRLENKLIYGFFD
                     DKQNKMQDLKYLGKINELNSWADSKKKIYKFIVAIGDCVVRKKIIKKLNMPSEQYGIA
                     VHPSAIVAKEISIGYGTVIFANAVINPDAVIGEHAIINTGSIVEHDCRINNYVHLSPG
                     VCLAGGVHVGVGTQIGIGSQCIQMKKIGSWCMIGAGSTIVKDIPSHSLAYGNPAKIKK
                     EGINFEF"
     gene            93456..94598
                     /locus_tag="IWO89_000189"
     CDS             93456..94598
                     /locus_tag="IWO89_000189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HAQ0454979.1"
                     /translation="MNSRILLSSPHMGGTEEKYVAKAFKDNWIAPLGENVDKFEESIT
                     KYTGSKGGASALSSGTAAIHLALRLLNVGKNDIVFAPSFTFIATVNPILYQGAVPVFI
                     DSEPNTWNMSPVALESALEEAERNNQLPKAIIVVNLYGQMADYERILAIANQFNVPVI
                     EDAAESLGSTYKGISSGLFGTIGIYSFNGNKIITTSGGGALVSSDKKYVEHSRFLATQ
                     ARDQAPYYQHSEIGYNYRLSNISAGIGRGQMEVLNDRVAKRREIFNRYKEAFSGIDRI
                     EMMPEVDGMVPNRWLSTLTINDYNSIDQVVNVINKLNEKNIETRALWKPLHLQPVFQG
                     VKMYYHDNNLSVSEKLFNSGLCLPSGSNMTDSEQEFVIDEFKKIFK"
     gene            94611..95588
                     /locus_tag="IWO89_000190"
     CDS             94611..95588
                     /locus_tag="IWO89_000190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HAQ0454980.1"
                     /translation="MNIGIIGLGSMGKRRLRLIQEHFNDILLCGVDTNEARRKEVEKE
                     FGIPTYNSLTEAKNLFEINSVFVCTSPITHEFIIKEALNLDCNIFTEINLLNEYYDEI
                     IEVAKKKDRLLYLSSTFLKRKEIQYIKSKVTHNKKISYQYHVGQYLPDWHPWEDYRNF
                     FVSNKKTNGCREIFAIELPWIIDTFGKVKSFTVMTQKLSNLDIDYSDSFTLIIEHETG
                     IVGTLSVDVVSRVAKRNLTIIGEKILIEWEGNPDSLFSWNEETSSMTDIQLYDEVMHD
                     NNYSHTIIEDAYLEEIKEFFTLLEKKMEPQYTFEKDKYIINLINDIEVN"
     gene            95588..96496
                     /locus_tag="IWO89_000191"
     CDS             95588..96496
                     /locus_tag="IWO89_000191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dehydrogenase"
                     /protein_id="HAQ0454981.1"
                     /translation="MMKVIFFGLGSIGKRHLKNLISYGKKNSINFDITAYSSSGKKEF
                     EEITYIQNRDDLDTHYDIAFITNPTFKHLETLNVIVEKVDYIFLEKPAFSESIDLTQY
                     NYDYKNIYVAAPLRYKNIMEVVRNSIQSKKVYSVRAICSTYLPDWRKGTDYRNNYSAF
                     KSMGGGVELDCIHELDYIINLFGFPKSVKKQFGKYSNLEINSNDLATYILRYDDKIIE
                     IHLDYFGRVPTRKFQLFTLDECINVDLLNNKISFEENIVKEMPESANDMYEKELKYFF
                     ENVVTGKENWNDLYHANEVLKIAEEK"
     gene            96497..97249
                     /locus_tag="IWO89_000192"
     CDS             96497..97249
                     /locus_tag="IWO89_000192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylneuraminate cytidylyltransferase family
                     protein"
                     /protein_id="HAQ0454982.1"
                     /translation="MEILFTICGRAGSKGFKNKNLKYLAGKPLVHFALAMIDIYQQKH
                     PEHNCTTCLNTDSNELVELTTKLNPSVKIVERKPELATDTVGKIEVIRDSFLQLERED
                     NIFDLIIDLDLTSPLREVSNLEELIDEHLKNLDKDVYFSVVSARRNPYFNMVKRIENE
                     VTIVNKSRYTSRQQTPEVFEMNASMYSFKPEFLKKSNYIFDGQCGIIKMRDYLILDID
                     SEEDFKWLEFLFPKFLEDSSELQDIYNYLYRR"
     gene            97254..98201
                     /locus_tag="IWO89_000193"
     CDS             97254..98201
                     /locus_tag="IWO89_000193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HAQ0454983.1"
                     /translation="MSCYLITGGAGFIGSTLANHLGEEHVVIVVDDLSMGKVENLDMT
                     KNITFIEGDVAEKSLMEEIMKANKFDYIFHLAAVASVADSVARPVETHRVNFESVLMI
                     LELIRKYQPDLKRIVFSSSAAVYGDEPTLPKKEESVIRPLTPYAVDKFAAEQYVLDYC
                     HLYDVPGSAVRFFNVYGPNQNPNSPYSGVISILVDRYKKQIVGEATSFTLFGDGSQSR
                     DFVYIDDVIQALLLVANEEAALGKQFNVGTGKATTLLELIHAINEILGVELTLEYKEE
                     RSGDIHDSLADITKIQSIGYQPKYGILSGMTNYLKTEIN"
BASE COUNT        32613 a        15923 c        20305 g        29447 t
ORIGIN      
        1 ttatacagga ttcgggtaat ttcgagagag ttgaacagtg atccgatcaa tgaaagtatc
       61 tttttcctgc gttagtgttc ctgtgtaacg atccgctacg tctttcatca tcattaagtc
      121 aaaacgttcc tgatcatcta attcttctgt gatgcctcga ctaaataggt ttgtatcgta
      181 atgtgcacta tgttcgcaac aaatgacgaa atgttcctct tgttcgtgta tcgacaaata
      241 aatttttcga tacagtggat tttcgatggc atgagtttct ctaaaggaat gctctaaagc
      301 gtggatcaaa agttgagcca gatcgtcatc gttgatagga agttcttcgg gaagatctgc
      361 ttggaaggtt atctgaatat tccgtttggc agctaacttc tggtaacgcg ctaagataag
      421 atttgtcacc tggtggttgc aaaaagaaca cagcatttcg aaattttttg cttcttctaa
      481 tatcttttcc acatagtttt tagcttttgt ttggtccgta tgcattaaat cttgcagatt
      541 ctccatctct tggacaaatt ccaggcgaag tgtatttact tcttggatat gctgtcgaag
      601 ctgctcataa tgttgctcca acaaatcatt tttgattttt aagaaatcta cttgtctccg
      661 atctttgtcg gtatttaaga tgtgatcgat tagattgtag ctgataataa cgattagaat
      721 gataatatat aaaatcgtag accaattgat cgttgtttga tagataccta atgccgtttg
      781 gatgtaaatc aagcaatgga taatggctac caagacgatc caaggtgtac aagctacata
      841 aaaacggtta ttcttatatg cttcgccaat gcatgcaagt gaagtgacta aagtgcttat
      901 aacactagca atattagcaa tcggcattaa ttcaggaagt tctgcaggag taaaagttgc
      961 ataaagcaga acgccgataa aaatcaaaag ttggaacgta caaaaaattc cgtaccattt
     1021 tttatagtaa aacattcggc tccaatagta gcagatgatg aaagctgacg ttaagataat
     1081 aaataaatga gataaaaaag aatgaacgaa tggttcaaaa agaataccac ttaaaatatc
     1141 ttctgataca gcttcaaatc ctaatgccaa agcaaagcaa cttaagagaa taagtgagta
     1201 ttcaagacgt ttttgtttat acatcaagta gatcgtatag aaaaaggtcc ctatagcgat
     1261 aaaaacagcg attatcatcg tcaatacttg cggcactgat tgtgaataaa taaaactaat
     1321 caatggtcca gttgttccta gataaacttt cggtggcagt ccagcatagt tctgataagg
     1381 agatgagact gtaagggtca atttttttcc gatataattt gctggtaaat ctatcacagc
     1441 caatgagagt ccgggattgc tttcttcctg ataatctttc cgttggtata aaatcttttc
     1501 gcctagatat acagtaatcc attgatggtt tcctttgatt agtaaatttg cttcgtccaa
     1561 cggtttttca agtgtcgttt ccataatgaa tgtttcgtct ttatccacgg taggaatcgt
     1621 acgagtgtac ttgctgtcaa acttaaaatc aggatcatct tttgtataga aaacccacga
     1681 atcttttatt tctttcattt gattgctgtc cgcaggagaa aaaataaaat aaaagaaata
     1741 agcaattcct gttaaaaata acaatgataa tataatgatt atgctccact ttatacgtaa
     1801 ataaaaagat tcttttttat tatatatgtt attaaaaaaa gacataaagg tattcctttc
     1861 taaaacttgt aatgttaggg taaacgtttt ttaaactaac gtcaattatt tataataaaa
     1921 aatgagataa agatgaggaa atagaattag aaacatataa ttattaaagg gaaacatttt
     1981 tacagctata atttgaaaaa aatttatcgt tattatatga ttaagaagga ttgaataaag
     2041 aaagaagttg aaaacgatac ctgattacaa aaaaataact tttataagtt ctataacgtt
     2101 gattatattt ttaattctta ttcttctcgt cgtcttggtt ccccgaattt ttggtttgac
     2161 tccttatatt attgaggaca aagaaatgag tccatattat gcacaaggaa atctgatata
     2221 tgtacgaccg aaaaatacaa atgagatttt agtcggagat attattacat attatgaaaa
     2281 ttcaggaaaa aaaatctcga cgcgtcgcgt aatagccgtt gaaaacaacc acgaagattt
     2341 ttatacaaaa gctgatacaa agacttatat tgagcctgga gtagtaaaaa agagaaatat
     2401 tatcggtagt cccatctttc agattccata tattggacta gtcctttcaa aaacggcatt
     2461 tgcatggatt caggggttat tctttacgat tgctttttgc ctaacaggaa tcacagcatg
     2521 gaataccttt gtagagttga aaaagaagcg gagacgaaca cagaaatttt tgtaagaatg
     2581 ttaaaaacat aaatgtattc cagagatcca acgactcttc tgaacatgaa acacgagaaa
     2641 acactcatgc agcttgtaga tttagtcagt tgttcgtata atgtgtgaag aggaggagta
     2701 gtatgcgcaa tgaaatgatg catcgtcctg tagtcaaaga agaactattg gattttatgc
     2761 gtacccagca aaaaaaatta ccgggtgagc taggcaaact ggaagaagaa gctcatgtag
     2821 cagaagtacc agttattccc catgaaacag ttgtattttt gaaattttta cttggacagc
     2881 ttcagcctga acgaatcttg gagatcggag cagctatcgg tttttcatca agtgtcatgg
     2941 ccacgacaat cggagaaaac gctcatgtta cgacaattga tcgtttcgat gtcatgatcg
     3001 agaaagctaa aaaaaactat gaacgtctag gactgacaga taaagtgact ttgcttgaag
     3061 gtcaagcagc agatatcttg ccggagttgt ctggtccgta tgactttatt tttatggata
     3121 gtgcaaaatc aaaatatatt gaatttttac ctgaatgctt acgtttgtta agaaaaggtg
     3181 gcgttttgat ggtagatgac atcttccaag gaggaacgat cttgctccca gatgaagaga
     3241 ttccccgtgg caaacgagct atccatcgta aattaaatga atttcttcgt gttgtgatgg
     3301 atcatcccga tttgacatcg acaatcttgc cattaggtga tggtgtgatc ttaatgacaa
     3361 aggaatcaga aacaatcgag ttgtgataca tgattatggc agcctgtaga taatcgattg
     3421 aatcgagggc tcaattcgaa cttgatgtaa aagtttctat atttgtaagt caattaaagg
     3481 atacaggaaa tctaaataaa caggagtagc atgaagtcaa tgtagctcct gttttcaatt
     3541 gataacctta gaacagctga aggagaatgt ctcgttttaa aagattgatt tagtagttgg
     3601 gccaaaataa ttcattattt gctaattaaa taactatttc gtattgacag aagcaattta
     3661 attatctata attattgatg ttccgctgat tcttgtcata gtagctcagc tggatagagc
     3721 atccgccttc taagcggacg gtcgggggtt cgaatccctc ctgtgacgct aaaaaaccgt
     3781 tagaaactga ggtttctaac ggtttttctt tgttttttgt gccaatattg tttgttttca
     3841 aaggcaaata aaataaaaaa acatccttat cgagtacaat tataaggagc aaaaaaataa
     3901 aactcatcag aaaagaggaa gaagatatga cgatactatc tagaatttta gtgactctag
     3961 ttgcaattga atttttttat atcatgtata tcgaaacggt gagaactgat tctgacacga
     4021 caagtagggt ttttaaaatg agtaaagaag agttaagcag aaaatctgtt caaacattgt
     4081 ttaagaacca aggagtgtac aatggactat taggagtggg actactttat ggagcttatc
     4141 tttcttccgc atctaaagaa atcacaagta tgctattaat atctattttt tttgttgcat
     4201 tatatggcag tttgactagc gatcgttgga ttttactcaa acaaggtggg ctagctctgt
     4261 tggcattgct ttctattttc ttataaatat agaaaaaata agaatttatc taacaggaaa
     4321 ctttgtgaaa aatattactt tatctcatga aatatgatat tctaatgaat ggtattttag
     4381 aaaatttcat gaaaaaggag ttagataggt gttcaaaaat tatgtttcct cactaaaaaa
     4441 agaattttca ggttatgatc tttcaagatt tcagaaagat cttttggctg ggatcacagt
     4501 agctgctgta gcattgcctt tagccttggc ttttggggta tcaagcggag ctgatgcggc
     4561 cgcaggtttg attacggcta tcattgccgg tatcgttatt ggcggtttat caggcggttt
     4621 ttatcaaata tcaggtccga caggtgcgat ggcagctatc ttgatgtcta ttgcggcaac
     4681 aaagggaatg caaggtgtat tgcttgctac ttttttagcg ggtctatttt tacttgcagc
     4741 aggacttttc cgattaggaa cactgacttc ttttatccca gcacccgtaa ttacggggtt
     4801 tacttcaggt attgctatca ttattgcatt aggtcagatc gataatttat ttggtgttca
     4861 ctcggaaggt acaaacgtta ttgaaaaact aagtagttat caaacgatag gatttccgat
     4921 aaacacagcc tctttgatca tgggagggct agtcatttta gggatgttta tctatccaaa
     4981 aaaatgggcg aaacgaatgc ctagttcgct gctggctatc attttggcaa cagcagttat
     5041 gctattgttc catttaccag tagcaactgt gggtaagatt ccacaaacat tgatcagtag
     5101 taatcgtttg aatatgggag attttagctt ttctgccttg caacaagtgg cagttccagc
     5161 tattagcatt gctttattag gaatgattga aagcttgcta tgtggtgctt cggctggccg
     5221 aatggcaaat aagcccttag atagcaatca agaattaatg gcgcaaggaa ttggaaatct
     5281 gttgcttcct ttctttggcg ggattcctgc tactgcggcc atcgcccgta caagcgtggc
     5341 catcaaatct ggtgcacaaa ctagattagc tggtatgata catgcagttg tcttactgct
     5401 ctccatgctt gtttttgcgc cagtgatgtc acagattcca atgccagcac ttgcaggtgt
     5461 tttgattgtg acggcttggc gtatgaatga atgggaaacg atcaaggagc tatttactaa
     5521 taaatattgg tcagcagttc tattgtttat ccttacgatg atttgtacag ttatctttga
     5581 tttaagtatt gctatcgtga tcggggtgat cggcggctgt gtattttttg ttgtcaaaag
     5641 cgctgctatc acgatttcat tagaaacgat cgattggcaa agaatggggc tgccagaaac
     5701 ggaaaagctg aataactgga cagtagtgta tattagtggt ccactgtttt ttatgtcttc
     5761 agaaaaactc aaaaataaat tggaagaact aaatcatcac gatggtgtca ttttctccat
     5821 gcgaggcgta ccaagtattg atttgacggc acgtgcagtt tttgatgaat ttcaagaaaa
     5881 agcagagcaa agaggacaaa cgattatcta tacttccttg cagccagaag tagaaaaaca
     5941 actttctcac ttgtgggaac atacattcac gcatcaacat caaacagttg ctcatgcgtt
     6001 gacagcttta catgcgaaat atactgcaat tagccattaa aacaatgaaa aagaacagac
     6061 aagactgtaa actgaagatt ttatggtctt gtctgttctt tttaatattg ctgtaaaagt
     6121 gaagctagta cttgccagtc ttgatggata acaggtacaa gggagcgatt gtagaaaata
     6181 gctgctggat gaaaaagtgg aagaattcga tagttttttt ctgaccattc gtaggcatcc
     6241 ttttgttctg ttaattgtaa gacaggccct gtatagagtt gaccatgatt tttggtaacc
     6301 gtatattctt tgccaagtaa acgttgaaga ccaatgtttc caagtgtcgc aattaatttt
     6361 ggctggacct tcatcagttc gtaatccaat attggtgcat gcgcaaatac ttcggaacga
     6421 gtaggtgtcc gatttggata aacagtttct gactgttgtg tccgtttatt gatccgatga
     6481 gtcacgcgat acgggcggct ccttacagca ctggtgatat acacctcttc tcgtgttaag
     6541 ccagttgaag aaagtgcctg catcaattct tttccagctt gaccgctaaa aggaacatgc
     6601 gtatcaatct ctgttttccc tggggcttct cccacgagca tcagtttagg atgaagaggt
     6661 ccttgcccag cgataaatcc ttctacagga cggccgttca ttcttaaaga agcttcttgt
     6721 atcagttctt ttgggtattc catttatgct cctccttttt cttttattat aagaataaaa
     6781 gaaacacaat gcgactattt tatttggtag aactgtgagg atagaaaagc agaagtattg
     6841 cttgaatttt tgttttactt tttgatttta aatttagttg actaacggtt gctgttctgg
     6901 taagctagta ctgttgtaat tgtggactcc acagctacaa ccgctcagaa caagtaataa
     6961 gcacagcata tgctgtcact tcggatggcg agtctaagta tatcagaagg aggtgcttta
     7021 tagcatgtac gcaattatta aaactggtgg taaacaagta aaagttgaag tgggacaagc
     7081 aatctatgtt gaaaaacttg acgttgaagc aggagaaaaa gttgtttttg acgaagttat
     7141 cttagtaggc ggagaatcta caaaagtagg cgctccaact gttgcaggtg caactgttga
     7201 aggtactgtt gaaaaacacg gcaaacaaaa gaaagtcgtt actttcaaat acaaacctaa
     7261 aaaacattca caccgtaaac aaggtcatcg tcaaccatat acaaaagtgg ttatcgacgc
     7321 gatcaacgct taatagcgtt tgagaaagaa ggcctatata tgatcaaagg aacatttaag
     7381 cgaaatgacg caggtcagat cgtttcattc actttgaccg ggcatgctga tgctggtcct
     7441 tatggaagtg atatcgtttg tgcgggtgtt tctgcgttag cgatcagcac tgtcaacggg
     7501 attgcctcat tagccggttt tgagccgatt gttgaaatga atgaagaaga aggcggttat
     7561 ctttataccg aagtcacttc aggaatgaca caagaacaaa acaatattgc tgagattcta
     7621 ttagaaaatc ttttattagg attacaatcg atagaagctg aaaactcaga atatatccaa
     7681 attaaaacaa ttaatgataa ataggaggtg cagaccatgt tgttatctat gaatctacaa
     7741 ttatttgccc acaaaaaagg tggcggttct acatctaacg gccgtgactc agaatcaaaa
     7801 cgtctaggtg ctaaacgtgc tgatggacaa acagttacag gtggatcaat tctttaccgt
     7861 caacgcggta caaaaatcta tccaggtgta aacgtgggta tcggtggaga cgacacttta
     7921 tttgcaaaag ttgatggcgt agtacgtttc gaacgtaaag gccgtaacaa aaaacaagtg
     7981 tctgtttatc cagtagctca agaagcttaa ttgaatctta tgattaaaga ggttgtgaca
     8041 agacttttgt cacagccttt tttgtttata aaaataaaag ttgtagtgga ggctacgaag
     8101 tttactttgt ctttttacac ttaatatttt tttgccaatt tggaatcagg atgttttgat
     8161 aactatcgtt ttcaattgat ggaagctata ttgtttgcta tcagtgaaat aattatatgg
     8221 taaacagtgt tctttttctt gtttccatta taaaaatcgt atcgggattt ttcgttcttt
     8281 taaattgaac atttctatct atccgcgtgt tatcaacaaa acaaaatcag gtaaatagtt
     8341 gacgaaacag aaataaatac atagtattat gttttgaagc ctaagtacaa tagtctatag
     8401 aaaaggagcg ataccattgg caaaacgcat tgaccgaatt tatatgtatg tcaaagaaaa
     8461 aaccgctcat ttaacaaagg cagaatatga tcaaggagta acaacacagg aaattgctga
     8521 agtgttaggg attcagcgaa caaatagcag taaagatctg aatcagctag tgcgcgaagg
     8581 aaaactgata aagacagacg gacgaccagt acgttacata tatcaaacac tcgttacgca
     8641 cgtaaagcca gttacaaaac atgtagtcag ttacaaagaa acgccggtct ctgaagaaaa
     8701 aaatacactc caaatagata ctaaggatat ctttgcaaaa atcattggag ctaacggcag
     8761 catgaaaaat tcagtggaac aggcaaaagc tgcgattttg tatccgccaa gaggattaaa
     8821 ttgtttgatt acaggaccta ccggatcagg aaagacttat tttgcacacg cgatgtatca
     8881 ttttgcaaaa gcgcatcatg taatagatga ggaaacggaa ctgattgttt tcaattgtgc
     8941 tgattatgcc aataaccctg agcttttgat gagtcattta tttgggtacg caaaaggtgc
     9001 ttttacaggc gcagatgaag aaaaaatggg gattatcgat caagcggatg gcggtatgct
     9061 gtttttggat gagattcacc ggttgccgcc tgaaggtcaa gaaatgatct tttactttat
     9121 ggatcatgga acttacagtc gtcttgggga aacaacgaaa tcccatgaag caaatgtacg
     9181 gatcgtaggt gcgaccacag aagacccagg ttcttctcta ttagaaactt ttgttcggcg
     9241 tatcccaatc aatatcaaac ttccggcttt tgaaaaacgt cctgcaaatg agaaagtcga
     9301 tttagtaaaa atcatgattg cccacgaagc aaaccgcacg cagcgaaaga tttctttgac
     9361 agaagatgta gcaaaagcat tgatcggcag tgtgacctat ggaaatatag gacaattgaa
     9421 gtcaaatatc cagttagtat gtgcacgagg atttctaaat catatgcaat cacccgaaat
     9481 ctcgatcaca atcgatgatt tgacagaagg gattcgtagc ggattgattc aattagcaag
     9541 caaccgcagt gcaatggcag agctttctaa aatattggag ccaaagataa ctgtagcacc
     9601 aaatgatacg atcatgaaaa tccaatcgga ctcctatgag ttaccttata atctatatga
     9661 tatcatcgga gataaagcag ctttactgaa gtcggatggt ttggatcaag aagcaatcaa
     9721 tcatttcatt tctacagata tcaacatcca tctgaagtct ttctataaag accatggttt
     9781 ttcttttaat gcagacagca aattagcaga atttgtagat ccgaaaatca tagaagtgac
     9841 caatcagatt tatgtaatgg tcaaaagatc tttaccatat gagtttcagc aaaactttat
     9901 ttatgctatg agcttgcata tcagttcttt tttgaaaaaa atcaacttag gagaagaacg
     9961 gcatacgaat gataacatcc gtgagatggc ttttgatttt ccagatgaat atgctgttgc
    10021 gaaagaagtg cgtagttata tcgaaacgta ttttcaagtg gagattccag aaagtgaaga
    10081 ctattacttg accgttctac tcgtctcgtt acgggcaaat caagcaagcg gcagaatcgg
    10141 tgtagtcgtt gcagctcacg gaaacagcac agcaagcagt atggtccaag ttgtccaaca
    10201 gttgttagat gcagagaacg tacgagcagt agacatgcct ttggatatgg atccgaaaac
    10261 agcattggac cgaattgaac gcaatgtaca agaagtagat gaaggaagcg gggtcatact
    10321 cttagtagat atggggtccc ttgcgtcgtt taattcgcaa attcaaagag acactggaat
    10381 tccagtacgt actgttgata tggtaacaac ttctctagtt ttggaaactg tacgaaaggt
    10441 ctcagtatta gggacagatc tagatatgtt gcatgaatca ttgaaaaatt ttcgaggtta
    10501 cgcagaaata tcttctgtag aatctgttca ggaagcaaaa cctattcata agaaaaaagc
    10561 tgttttggct atctgcgcat ctggcgaagg tacagcacaa agaatcaaag aattgatcga
    10621 acgagcggta aacaagcgtc aagaaacaga attagaagtt ctagctttgt cgattgtgga
    10681 agtgaaagaa gaactgccag ttatccaaaa agaatatcag atcatagcta ctactgggat
    10741 tactgatttg aatatccaag ccccatttat tcctttagaa cgttttattg atcaaaatat
    10801 cgaagtcatt ttggatcagt tgctgatgga atcagaattg gaggaaactg aattcatctc
    10861 cctagatgaa gagtcagcta aaaatacgtg tgttgagttt atctctgata acttcatctt
    10921 tattaatggt agtaagttaa tcgatccaat gtggcaattt tctacacaaa tctctcagac
    10981 gacgggaatc ggcgatgaag aatatggatt caaaatcaac ctagtgatgc acacagcagg
    11041 aatgatcgaa cgtattattc ggaatgaacc attgactgtg gaagaaaacg aattgacaaa
    11101 tacaacgaat gatcctcttt atagccaatt ggcagcctca gttgttctgt tggaagatca
    11161 gataaaagta aaagtgccga tagaagaaat gtattatctg ctacgtctag tccataatca
    11221 gttagataaa aaagaataca ctgtgccatg aatacacaaa aacgatacac ttttatagtg
    11281 tatcgttttt gtgtattggt taagaatcct ttaaattcaa ttagtgtatc gtaaaaaagt
    11341 tggcacgctt tttgcttata gtagtgtgtg ctaggaggtg gttgttcaca cagaaatgat
    11401 aggggtttct gtgaagtagt gaggaagaag gagttttgca gatggatatt cgattggcac
    11461 gtatagatga tcgtttaatc cacgggcaag tcgcaacagt ttggtcgaag caaacaggtg
    11521 ttgaacgtat cattgtgatc agcgatgtag tcgctcaaga taaattgaga aagtttctac
    11581 taaaagaagc tgcaccgccg gggatcacag caaatgtaat aaccgtgcag aaaatgattc
    11641 aaatctatca tagtggatta ttatcacgag caaaagtgat gcttttattc accaatcccc
    11701 aagatgttga agcggttgtc cgctgcggtg tacagttgaa gtcgttgaat atcggcggta
    11761 tgagttttac tgaaggcaaa tcaatgatta ccaattttgt ttcagtaaat caacaagata
    11821 ttgattcctt tcgttacttg aacagtcaag aagtggagtt agagattcga aaagtacctg
    11881 ctgacaggaa aatccttttg atggaactac tagcgaaaat caaaaaggtg tagcaagaat
    11941 ggttgttgga aaaggtcaaa taattctgtt gtaatggtta gcaattatta atatttttga
    12001 atttgttaca acaaaagtaa ataggaggta taaatatggt agggattatc ctagcaagtc
    12061 atggacaatt tgctgaagga atcttacaat ccggttcaat gatctttggc gaacaagaaa
    12121 acgtcaaagc cgtgattttg aaaccaagtg aaggtccaga tgacttgaga gccaaattgg
    12181 aagaagcggt tgcttctttt gataatcaag acgaagtgtt attcttagtt gatttatggg
    12241 gcggaacacc gttcaatcaa tcaaacacat tattcgaaga gcacaaagat aaatgggcaa
    12301 tcgtcagcgg tttgaattta ccaatgttga ttgaagccta cgcatcacgc ttttcaatgg
    12361 aatctgctca tgaaattgca gcacacatca ttgaaacagc aaaagacggt gtaaaagtaa
    12421 aaccggaaga attagaaccg gcagaagcac cgaaagcagc agttgaagac gcacaaccta
    12481 aaggagcact tccagaagga actgtggtcg gagacggaaa aatcaaatat gtattagcac
    12541 gtgttgactc acgtctgtta catggacaag ttgcgacagc ttggacaaaa gcagtgcaac
    12601 caaaccgtat tatcgttgtt tcagatgcag tatcaaagga tgatctgcgt aagagactaa
    12661 tcgaacaagc tgctcctcca ggtgtaaaag caaatgttat tcctattagc aaaatgatcg
    12721 aagtagcaaa agaccctcgt tttggaaata ccaaagcatt gcttttattt gaaaatcctg
    12781 aagacgtatt gacagctgtt gaaggtggcg tagacatcaa agaattaaat gttgggtcaa
    12841 tggcgcattc ggtcggtaaa gtcgttgtaa gtaaagtatt gtctatggga caagaagacg
    12901 ttgaagcgtt tgagaagctt gaacaaaaag gtgtgaaatt cgatgtacgt aaagtaccta
    12961 atgattcacg cgataacatg gatgatattt tgaaaaaagc aaaagctgaa ttagcaaaag
    13021 cgtaataaat aatattaaaa tttataggag gcttatcatg tctattattt caataatttt
    13081 agtcgtatta gttgcctttc tagctggtat ggaaggtatt cttgatgaat ttcaatttca
    13141 tcaaccacta gtggcatgta cattgatcgg tttagttacc ggtaacctcg aagctggtat
    13201 cgtacttggc ggtacgcttc agatgatcgc tttaggttgg gcaaatatcg gtgccgctgt
    13261 agcaccagat gctgcattag catcagtagc atcagcaatt attttagttt taggtggaca
    13321 aggtgttaaa ggtgttcctt ctgcgatcgc gattgccgtt cctttggctg ttgcagggtt
    13381 gttcttaaca atgatcgtac gtacaatcgc tgttccaatc gttcatttga tggatgcggc
    13441 tgctgaagaa ggaaacatcc gtaaggttga aatgtggcac atcattgctg tatgtttaca
    13501 aggggttcgt atcgcggtcc cagcagcagc gttgctattt atcccagcac aaactgtgca
    13561 atcattcctt gaatcaatgc cagcatggtt aacagatggt atggctatcg gtgggggaat
    13621 ggtcgtagct gtaggttacg ctttggtaat caacatgatg gctacaaaag aagtatggcc
    13681 attcttcgtt attggtttcg ttgtagctgc aatttctcaa ttaacattga ttgctctagg
    13741 tgctttaggt gttgcattag ctcttattta cttgaacctt tctaaaatgg gtggttcatc
    13801 aaataatgga ggcggaagca actctggtga ccctttaggc gacattttga atgactatta
    13861 atcttgaagg aggaaaataa aatggcagaa gaaaaaatca aattatcaaa aaaagatcgt
    13921 ttagctgttg cgtggcgctc taccttcatt caaggttcat ggaactacga acgtatgcaa
    13981 aacggtggct gggcattttc tatgattcca gcaatcaaaa aattatacaa aacaaaagaa
    14041 gaacgttctg cagcattaaa acgtcacttg gaattcttta atacacaccc atacattgct
    14101 tctccaattt taggggttac attggctctg gaagaagaac gtgcgaatgg cgcgcctgtt
    14161 gatgatgtag cgattcaagg ggttaaagta ggtatgatgg gtcctttagc tggtgttggt
    14221 gacccagtgt tctggtttac agtacgtcca atgctaggtg cactaggtgc ttcattagct
    14281 atgggaggaa atatccttgg accaatcatc ttcttcttag gttggaacat tattcgttgg
    14341 gcattcatgt ggtatacaca agaatttggc tacaaagctg gttctaaaat tactgatgac
    14401 ctttctggcg gattgttaca agacgtaaca aaaggtgctt caattttagg gatgttcgta
    14461 ttagctgccc ttgtacaaag atgggtatca atcaaattct taccagttgt atcaactgta
    14521 aaattagata aaggtgctta catcgaatgg gataaattgc cttcaggtgg tgaaggaatc
    14581 aagagtgcat ttgaacaagt taataatggc ttagcccttt ctcctgaaaa ggtaacaaca
    14641 ttacaagaca acttagacca attgattcct ggtttagctg cattaggatt aacattgtta
    14701 tgtatgtggt tgcttaagaa aaaggtttct ccaatcgtta tcatccttgg tttattcgta
    14761 gtcggtgttg ttggacacgt catcggactt ttataatcca taaaaatata gctcgggcgt
    14821 tgtcccgagc tttttttata tagtatactg ttcataaaca aatatctgga gggagaaaat
    14881 ggttcaatca atcaatacaa aagtagactt ggtaattgat gctacttcgt ttacgggtct
    14941 gacagattac gggcaaatca tgataggtga taatggtttt gagttttacc atgcgcgaga
    15001 tagacgcaaa tatatccaaa ttccatggga agaagtggat tatgtactag catcagtcat
    15061 gctaaaggga aaatggattc ctcgatatgc cattcaaacg aaaaaaaatg gtacatacac
    15121 attttcttcc aaagaaccta aaaaagtgct tcgtgcgatc agagaatatg tagatgaaga
    15181 tagaatggta caatcattga gtttctttga tgtagtgaaa cgtggtattc gtagtatgtt
    15241 taaacgcaag taaaaaataa agaaaacttc taagaggagg ttttctttat ttttttactt
    15301 tgcagaagaa aatatgaaat agcaaaacca attatttgtc ccctttaaat cccatatgct
    15361 aaaattgatt agcaaagaag gaggttatat tatgagttgg ttatggacat tgattgttgg
    15421 agccgtgatc ggagcaattg caggtgcgat caccagtaaa ggaaattcga tgggctgtgt
    15481 aacgaatatc attgctggac tagtcggttc aagtattgga caagcgattt tcggcgaatg
    15541 gggaccaaaa gctgcaggta tggcacttat tccatctatt ttaggcgcag tgattttggt
    15601 agcagttgtc tcatttttct ttggaaaacg atcataagtt tttacaaaat agtaaactaa
    15661 aaggagttgc agcgagactg tgactccttt ttggctttat ttatttaaaa taaaaagaag
    15721 ttttccggaa atttttattt aactgtcgtg tacgaagaaa agtttgctat aatggaaaaa
    15781 agcgtgcaaa gaaagaggga gtttcattat gatgctacgt gtagaaaaat tgcgcaaaaa
    15841 aatgcaagaa gaaaatttgg attcattttt ggttaccagt ccgtacaatc ttcgttactt
    15901 aacgaacttt acaggtacga caggattagc tgttattact ttggaaaaag cattttttat
    15961 tactgatttt cgttataccg agcaggcggc agcgcaggct caagggtttg aaattattaa
    16021 aaatgtcgga cctatttttg atgaggtagc aaacttagta cgcaaagaag aactgcactc
    16081 attagctttt gaggaaacaa cagtctcatt tttagaatat tcggttttag aagaaatcat
    16141 cgatgcggaa ttagtacctg tttcacagat gatcgaagaa ctcagagaag tgaaagatga
    16201 agaagaaatc gctattatcg aaaaagcatg tcatattgcg gatatggcat atgatcacat
    16261 cctgaaaatg atccaaccgg gcatgactga gattgaagtc gcaaatcaac tagactttta
    16321 tatgcgttct cttggcgctt caggtgtttc ttttgatacg atcgttgcta gcggattgcg
    16381 ttcggctatg ccgcatggtg tagcaagcaa aaaaatcatc gaacaaggtg acttgatcac
    16441 attggacttt ggttgctatt acgaagggta cgtttccgat atgacacgta cattcgctat
    16501 tggtgatcct ggggaaaaat taaaagaaat ttatcaaata gtcttagatg ctcaattagc
    16561 agttattgat gctgcaaaac caggaatgac tggtgtccaa ttagatgcag ttgctagaga
    16621 ttatatttca aaacatggtt acggggaagc atttggacat tctacaggac atgggatcgg
    16681 tttagagatc cacgaaggac caaacgtttc ttctcgtgca gaaaagcaat ttgtaccagg
    16741 aaatgtgatc actgatgaac cagggatcta cttaccagga atcggtggag tacgtattga
    16801 ggatgacttg ttgatcacag aagatggaaa tcgtgtgttg actcgttcac caaaagaatt
    16861 gatcatctta tagtttcatc aatagaagaa gtaagcgtaa aaatcagtgt tcacagactt
    16921 tgttatagaa aatcactcga tttaatgata cactatagaa gaacttttaa gctgtaagag
    16981 ataataaagt tgggtgtttt ctccaattca tagcagtttg cagctgatcg gcggcagttt
    17041 tcttaccacg tgatcactat gccgcatcat agagaattcg gatgtatctc gtgcttaggc
    17101 aaagggagga ataaataaat ggcagatgaa aaaaatttga ttttaaacgc aaaccaagat
    17161 ttaggagaga tcgtgatcgc tcctgaagtc atcgaagtaa ttatcgggat tgcagcttct
    17221 aaagtagaag gcgtatacgg aatgcgcgga acttttgcca gcaatgtcac tgaattatta
    17281 ggacgtgcag ctcacggaaa aggtgtttac ttagcaaacg aggaagatgg cttgaaagta
    17341 gatctttact gttatctaga atatggtgtt tcagttccta aagtagcaat ggaaatgcag
    17401 gaacgggtca gccagcaagt ccttttcatg acggatatcg atttatctga agtcaatatc
    17461 catgtagtag ctgttgttcc tgaaaaatta gaacaaccaa caatcgaaga actttttgaa
    17521 gacgaagagg aagagaatga ataaagaatt atcaagacac gagattcggg aaatggcatt
    17581 gcaggcttta tttccattag acttcaatgc agacctaaca aaagaagatg cgattttcaa
    17641 cgcaatcgaa ttagaccacc gcgacatgat caacgaagat gaatcagagt ttgttcctgt
    17701 ttatcttgat acgctagttg gcggtgtctg tgcaaaaaaa gatgaattgg ataaagtgat
    17761 tgaaaaacat ttaaaaaaca attggcacat caatcggatc tcaaaaatgg atttggtgat
    17821 cttgcgtatc gcgatttttg aaatgactta tgtgacagat gtaccagcgg ctgttgcttt
    17881 aaatgaagca atcgagttgg caaaaacatt tagtgatgac cgttcacgta aattcgtcaa
    17941 cggtgtttta tccaatgtat tgaaagaatt agaagctggg gcgtaagtcc tagctttttt
    18001 ctatgaatca tcataaacct gttataaatc gggaaaattc tatggtagaa tgaaaacaca
    18061 aaaaatatct gggagtggtt agatggcaga attgattaac ggaaaagaat tagctgaaaa
    18121 aatgcaggca gagattgctg aaaaaatcca aaaattgaaa gataatggta tccatcccgg
    18181 attagtcgtt ttattggtag gggaaaatcc agccagccaa atctatgtaa gaaataaaga
    18241 acgtgtagca aaagaaatcg gaatccattc attggtagag cgttatcctg agacgatttc
    18301 tgaagacgaa ctacttgcag aaattgaaaa atacaatcaa gatcctagtt ttcatggtat
    18361 attggtccaa ttaccattgc caaagcatat agatgaagaa aagatcctgt tagctatccg
    18421 ccctgaaaaa gacgtagacg gttttcatcc gatgaattta ggcagactcc ttgcgggtaa
    18481 accggacaag attccttgta ctccatacgg catcatgaaa atgtttgaag cttattctat
    18541 cgatccggcg ggtaaacgag cagtagttat cggacgaagc aatatcgtcg gtaaaccgat
    18601 ggctcagcta ctcttgatgg cagatgcaac tgttactgtt gctcattcta aaaccgaaaa
    18661 tctggctgaa ctggcacaag aagcagatat tttagtcgta gcaataggac gtggacattt
    18721 tgtcacaaaa gaattcgtaa aaccaggtgc tgtagtgata gatgtaggga tgaatcgtga
    18781 tgctaacggt aagctgatcg gtgatgtgaa atttgatgaa gtggagccta ttgcaggctg
    18841 gatcactcct gtgccaaagg gtgtagggcc aatgactatc acgatgctga tgtatcaaac
    18901 cgtaaaatct gccgaagctt ttggagcagg tgaatagatg gcacaagaat atttaactgt
    18961 cactacatta acgaagtatt tgaaaagaaa atttgatgcc gatccttatt tagaacgcgt
    19021 atatttaaca ggcgaaatat cgaatttccg tttacgtgcc aatgcccatc aatatttcag
    19081 cttaaaagat gaccatgcaa aaatatctgc tattatgttc aaaggagcat tccaaaaatt
    19141 acgattccag ccaaaagaag gaatgaaagt ccttgtcgtt gggcgtattt cattatatga
    19201 agcgggaggt tcttatcaaa tctatgtaga gcacatggag cctgatggaa taggtgcttt
    19261 atatcaagaa ttggaagaac gaaaagaaaa gctgatgaag gaaggccttt ttcaagggcc
    19321 taaaaaaatg ctgccacgtt tcccaaagcg aatcgcggtc cttaccagtc caagcggagc
    19381 ggttatccgc gatatcatca cgacggtgaa aagaagatat ccaattgtcc aattggtttt
    19441 gttcccaaca gttgtacaag gaaaccaagc agcggacaat gttgtcagga atatccagag
    19501 agtagaagag atgggcaatt tcgatacaat gatcatcggt cgtgggggtg gttcgattga
    19561 ggatttatgg ccgtttaatg aagagcgggt tgctcgggcg atttatcaag ctaatacgcc
    19621 ggtgatttca tctgtaggac atgaaacaga taccacgatc gctgatttag tagcagatgt
    19681 tcgggctgcc actccaacag cagctgcaga attagctgtt cctgttttgt cagaagagtt
    19741 gatgcgcatt caagaacggc aagctagatt gcagcaggcg tttgtacgcc agatccagcg
    19801 caaacaagaa cggttcgagc gtgctcgacg ttcctatgta tttcggcagc ctgaaagatt
    19861 gtatgaaggt cagaccatca aattagacca gttgaaacag cgattatatc aagctacaca
    19921 gcaaatttat catgcgaaag aaaaacaaac gatcgcatta agccaccaat tggaacaagt
    19981 ggctccgctt tatcgagtta aagcagctcg ccaagaaact gcttatctag aaaaacgctt
    20041 agaagagaaa atccaacaat atatgaaaca acagcagcag cgttttgctc aagctgttca
    20101 atctcttgat ttgttgagtc cattgaaaat catgggaaga ggctacagct atacgacgtt
    20161 aaatgatcaa gtagtaaaaa gtgtcaccga gattcatcaa ggggatttgc tgacgatcca
    20221 ttatcaagat ggaatcgtta aaggacaagt acaagagata gacaaaggag aataaaaata
    20281 tggaaaatcc aacttttgaa gagtcactgc aagaattaga aaaaattgtc atgcagttag
    20341 aacaaggaga cgttccatta gaatcagctc tagattcgtt taaacgagga atggaattaa
    20401 gcaaacattg ccaagataca ttagctaaag cagaagagac cttgacgaaa atgatgacag
    20461 aatcaaatga agaagttgat tttgacggaa acgaggaaag ctgagatgag attaactgac
    20521 ttttcagcta ttcatctacc tgctgtagaa aaagaaatct tgtctttttt agatgagcat
    20581 acaacggata gaggactcta tgatgcgatg agttattcag tgaaagcagg cgggaaaaga
    20641 atacgtccct tattactttt aacggccgtt gcttcctttg atgagcctat tgatgttccc
    20701 gtctatcaag tagcagctgc tttagaaatg gtgcatacgt attccttgat ccacgatgat
    20761 ttgcctgcga tggacaatga tgatcttcgt agaggaaaac ctaccaatca taaagtattc
    20821 ggcgaagcat tagctatatt ggccggagat ggtttgctta caggagcatt ccagctaatc
    20881 agtatggcac acttaggtaa ttctcctaag cttctgctgc ttcagcaatt agctgtttgt
    20941 gcgggatcac aaggtatggt tgctgggcaa gctgcagata tcgagggcga atcgaaaaag
    21001 ctttctttag aagaattagc atttattcat gaaagaaaga cagggcattt gattcgttat
    21061 gccttgctgg ctggtggcat attagcgaag caacctgaag aaatcttatt gctacttcaa
    21121 cgattagccg aacatttagg tttagctttt cagataagag atgatctact cgatgtgatt
    21181 ggtacaacga aaacattagg gaaaacagct ggtaaagatg aacgtatgga aaaaaatact
    21241 tacccgcgtt tattaggcct agaaaagaca cgtgaggcgt tggagattga gctgttatcc
    21301 gccaacaaga tcatagacaa attagaagag gacgtttttt cttttgatgg cgggctgatg
    21361 cgccagatga tcaagcaatt tgatgtggag aaatgaggga aagaatggaa aaagaacgcg
    21421 tagatgtatt agcggtcaaa caaggattat ttgaaacaag agaacaagcc aaacgttcag
    21481 tgatggctgg tttgatttac aacgaaaaaa acgagcggtt tgataaaccg ggagaaaaaa
    21541 ttccagtgag tagtgaatta agagtcaaag gtaaaaaact tccttatgtt tctcgagggg
    21601 gattaaaatt agaaaaagca ctcaaagcat ttgatctatc tgttgaagga aaaacattgt
    21661 tagatattgg tgcatccact ggcggattta ctgatgcagc tttgcaaaat ggcgcaaaaa
    21721 tgagttatgc tttagacgtt ggctacaatc agctagcttg gaaaataaga caagatccta
    21781 gagtcgtcgt aatggaaaga gtgaattttc gttatgcaaa acccgaagac tttaccttag
    21841 gaattccaga agttgctgtt atcgatgttt cttttatttc actaaaactg atgctgcctc
    21901 cattacatgc tattttaaaa gaagagggag aagtgattgc tttaatcaaa ccccaattcg
    21961 aagcaggcag agaagctgta gggaaaaacg gaattgttcg tgaccctcag acccatcaaa
    22021 aagttttaga agacattttg tcatttgcgg cacaaggtgg atatgatgtg ctggaattaa
    22081 gttattctcc gatcacgggg ggagaaggaa atatcgagtt cttggctcac ttgaggaaag
    22141 tacccgaaag cggaacaatc aattcagcta ttaatatggc agaagtggtt tcaaatgcac
    22201 acgaacagtt tgaccataaa taaattgtaa ctgtgaaaat caagtgagaa gtatgcggat
    22261 tcttctttat tataccaagg aaataagcta tcattgatta agaagaatat gctaaggagc
    22321 gaagacgtaa tgagaaaaaa agatcggcat cgtttgatta ctcgattgtt gagtgagcaa
    22381 gatatccgaa agcaggaaga atttgttgaa atcctgaaaa gcaaaggaat atctgttact
    22441 caagctacga tttccagaga tataaaggaa ctgaaactga tcaaggtacc ggcttcagat
    22501 ggcggctacc gttatagcat accagctgaa acaaacgaag atgtaaatac taaattggaa
    22561 aaactattaa aagacgcttt cgtggcagtt gaccaaatgg aaaaatttgt tattttgaaa
    22621 acattgccgg gaaatgcttc ggcagcagca aatttgattg ataaacgtta caagaaagaa
    22681 ttattttcga ttatcaatga tgacgataat gttttgatga ttacacgaac tgaagaccag
    22741 gcagttttat tgaagaaaga cttcctgcat tacctttaag ttgaggagcg aatgtgatgc
    22801 tacaagaaat cagtattact aactttgcga ttattccgga attgcggctt tcttttcacg
    22861 aaggaatgac cgcgctgacc ggagagactg gtgcgggtaa atccattatt atcgatgctt
    22921 taggtctgtt agcaggagga agaggttcaa gcgattatat acgtcaagga gcagaaaaat
    22981 gtgtgcttga agggttgttt gaactaccaa aacaagaagg attctctgaa ctgatggttg
    23041 aattagggat cgaaacagat gaggacaatc tgattgttcg acgggatatg tccttaactg
    23101 gaaaaaatgt ttgtcgagtg aatggtcata ttatcacatt agctaatctg agaagaatcg
    23161 gcagttattt agtagatatc caaggacaaa acgaacatca ggaactgctc cagcctgaat
    23221 cacatcttgc tttattggat cgttttggag atgtagcttt ccaacagaag aaaaagagtt
    23281 atcagcaaga atatatctct tatcgtgagc tggaaaaaag ggtacgaaaa gtgcagcaaa
    23341 atgaaaaatc ctatgttcag agaatagata tgctccattt ccagcaagaa gaaatagctg
    23401 ctgctgattt gcaagtcgga gaagaagaga aattaaaaga agaaagagaa aaacttagca
    23461 attatcaaaa aattgctgat ggattagctg caggttacgg agcactgacg aataatgaac
    23521 aaaacagtgt agatggtgtt ggcttggccg tttcagaact gcaagggatc gcccatctag
    23581 atgtggagta tgaagcaatt tatgaaaata tccaaagtgc ttactatttg ctgcaagatg
    23641 caatcggcga tatgagtcgt cagattgatt tattggagtt agatgagagt cgtttagaag
    23701 aagttacgca acgtttagaa ttaatccgac agttgaaacg taaatacggc gaatccattg
    23761 agtcaatatt ggcttactat gacgaaatca ccgaagagct agcttcttct gatttttcag
    23821 aaggtcaatt ggacaaaatg aaagaggaac tagagcaaaa agagcttcta cttcagcaac
    23881 aagccgcaga tcttcatgaa gcgcgtaaaa aaatcgcaaa agaattggaa aaatccattc
    23941 tgcatgaatt gaaaagtctg tatatggaaa acactgagtt tgaggtacgt ttcttaaaag
    24001 aagaaaatag acaattgaat catgatggat ttgaccaaat cgaattttac attacgacca
    24061 atccaggaga gccactgaag cctttggtga aagtcgcttc tggtggcgaa ctgtctcgga
    24121 tgttgcttgc tttgaaaact attttttctt ctgagcaagg tgtgacaagt attatttttg
    24181 atgaagtgga tacaggtgtg agcggaagag ttgcacaggc catcgcagat aaaatttctc
    24241 aaatatcgaa atactctcaa gttttgtgca tcactcatct gcctcaagtt gctgcagtcg
    24301 ccgactatca atattatatt gtgaaagcag tgattgatgg acgtacgcaa acttctgttt
    24361 cagagttgaa aacaaaagaa agagaagaag aaattgcacg tatgctggct ggaagtgaga
    24421 ttacaaaact cacactcgaa catgcaaaag aactattgaa actcgcaaaa aggacctgag
    24481 ccacctcgca gtggttcagg tccttttcag cagctgatag ctatatttta tttttacatg
    24541 acactcatta cagcagtcaa tacaacagat ccaatcgtta aaatcagcat tgtccaaatg
    24601 acaaattttg ttactttagc aaaagtactt gtttgttttt tttcacgcat tgttttcaac
    24661 ctcgtcatta attagttgat tcaagtgtac aatctttttt ccatttccac aagagggctt
    24721 ttttataaaa acttcccttt ttttaaataa taggtagtta cgatacaaat aaataatgtt
    24781 aatagaacta gccatataaa ggcatcatcc ctttcagcaa taggtaactt aatgttcatt
    24841 ccatagatac tgccgacgat tgtcggtata gttaaaataa tcgttaaaga agttaaaatt
    24901 ttcataatat tattcagatt attcgatacg attgctgaaa aagtctgatt catttgttct
    24961 actagtttta attgaatgcg agaagtagtc attgcctgct ctgtttccac caatacatca
    25021 cgtaaatgat ttttcaaagc atgattttct tggaaaagtt tattttgcgc tagttctttc
    25081 aatgcatcta aattagcaga agtcgcttct ctgaaataaa cgaggctttt ttggatatcc
    25141 atgatttgat aaagttgttt gttttcagtg gatacttgaa gtttggcttc caaagtatcg
    25201 cattgagatt gaagcgtagc taaataactg ttgtaagaga gtgtgacctg ccaaatgaat
    25261 tgaaagaaga tattgagatt aggcgaaata tcactttttg gaaaggtctg gctaaagact
    25321 ttctttaaaa acagtggttc atgattcgta attgtgataa gcttcttttt gggcgtcaaa
    25381 ataatagaga gcgggtatgt atccacttga aaataaccgc taggacttgt tttagcaaaa
    25441 ggatattgta acaataataa cgctggcttt gtcaactttg attgattgaa ttcttctgta
    25501 cgggaatttt ctgcatcgtc caatatggaa gtgatgtaat ctgacggaat ttcaaattga
    25561 tgtgtcagct gatgaatctc ttcttgatta ggacgttcta cacaatacca gatcgtttgg
    25621 tcagtttcct ttccggtatt acgcagtttc tcattatcta atgtaaaata tttgatcatg
    25681 attttcctcc tgttgcctgt ttcttttatt ataacggata gggaattgga atacatggaa
    25741 tgtgtttgta agatatagaa tataactgca taaatttaaa taaaatgaaa cagttgtgta
    25801 aagtatatac agtatacttg aagcaacgat aaaaaaggag tttacgttca tgggcacatt
    25861 cacaccactt atcttgtttt ggtatattta cccggtaatc gttttgtttg gttgccaatt
    25921 tcttgtaaaa ttattttcgt taaatcgtcg ttttaaaata aaagcccctg atttggcgat
    25981 tccttttttg tggggcggta tgcatgcatt gtctagaaat acagggacaa tttctatctt
    26041 accgtttttc tttatttctg ttttgctaat gggaatttgt attgctgttt tccaagcata
    26101 ttattatgaa gaaattattt atccgcgtta tttcaagatg acttggcgtt tggtttttct
    26161 gttgacaatc gtcttgtacg ctgtcttgat cattgtgaac atactttctt acctatgaaa
    26221 aaaatagaaa agctgtaaaa agtcttttaa aaggcttttt gcagcttgat ttttaaaaaa
    26281 atgtttatga ataaaaaaag ttgttcccgg atttcccaga aaaggtggta gaaagtgggg
    26341 gattgtggta gactaatttt agttagtgga ggaatggggg gctttcaatg ttgatgggtg
    26401 aattccaaca taatattgat gcaaaaggac gtttaatcgt gccctcaaaa cttcgtgaag
    26461 aacttggcga aaagtttgtt ctgacacgcg gattggatgg ctgtcttttc ggctatccga
    26521 tgagcgaatg ggaaaatctg gaagctaaac tgaacgaaat gccgttggct aaaaaagacg
    26581 cacgtacttt tgtgcgattc ttctattcgg ctgccactga atgcgaactt gataaacaag
    26641 gacgaatcaa tatacccagc acgttaagaa attatgcagc tttgacaaaa ggttgcgtag
    26701 tcatcggtgt atctaaccga attgaaattt gggatgagac acgatggcaa gatttttctg
    26761 cagcagcaga agagaacttt gacgaaattg ctgaaagtat gattgatttc ggactataag
    26821 aataggaaga ggctgactaa tgactgaaac atttcaacac tatacagtca tgttgaaaga
    26881 aacggtagat ggattgaaca tcaaaccgga tggcgtttat gtagactgta cactcggcgg
    26941 tgccggacat agtgaatatt tattaagcca actaagttct aaagggcatc tgtatgcgtt
    27001 tgaccaagat caaaaagcaa tcgatcacgc caagctgcgt ttagcttctt atgtcgaaca
    27061 aggtatggtc acatttatca aagcgaactt cagagaatta gaagaacgct tgagtgagca
    27121 tgtagataag gtagacggca ttttgtatga tctaggggtt tcctctcctc agctagatga
    27181 agcagaacgt ggatttagtt atcatcaaga tgcaccgttg gatatgcgca tggatcagtc
    27241 agctccttta tccgcttatc acgtaattaa tgaatattcc taccatgaat tagtaaagat
    27301 tttcttccgc tatggcgagg agaaattttc caaacaaatt gctcgtgaaa tcgaacgagt
    27361 acgaaaaaca aaaccaatcc aaacaactgg tgagcttgtc gaaatcatta aacaagtcat
    27421 cccagctccc gcaagaagaa aaggcgggca tcctgcaaaa agaatttttc aagctgtgcg
    27481 gattgcagta aatgacgaac tcggtgccga agaggcctct ttagaacagg caatccggtt
    27541 attgaaagtc aatggaagaa tcagcgtaat cacattccat tcattggaag acagaattgt
    27601 caaaagtatg tttaaagaat atagtacggt acaagatcta ccaccgggac ttcctgtcat
    27661 acctgacgaa ttccaaccag aactgaaagt cattactcgt aaaccaatcc ttccttcaga
    27721 ggaagagcta acagaaaaca atcgatcacg cagtgcaaag ttacgtattg cagaaaaaat
    27781 aagaatgaaa gaggagtaga atgatggcag aattaaagaa aatgcaagat tacgaatatg
    27841 atttacctga attggtggat cagccggaac aacaaatgcc tgattcttct tcgaatcaag
    27901 aaatcattct tccacaatca ccggcaaaac gattaaagca gatttctgct ttggaaaaaa
    27961 ttgctgtggc aagcgtggcg attgctattg ttgctttatg tgttttaaca gttatgctgc
    28021 gcacaaatat cagcggagct gaaaaagaaa tcacgtcaat acaaacagaa atcaatgata
    28081 aaaagcaaga aaaaacgagt ttagaacaag aaaaaaacga actttctcgt actgaacgga
    28141 ttaaacaaat cgcagaagaa aaaggattgt ctattaatga cgacaattta aggaaagtga
    28201 agtaaatgag tcttaaaaat aaaattcggc ggtttatgga aaagaaaaat ctaaatccga
    28261 tgaacaatcg caaaaaagta ggtatcattt tatttgctac gagtattgga ttgttctttt
    28321 tatttgccgt ccgtttctcg tatatcgtta ttggaggtca tgttgctggt acctcattag
    28381 ctgaaaaaac aaaacagttg taccaaggaa gtgaagtagt aaaagcaaaa agaggaacga
    28441 tttatgatcg taacggtgtg gcgctcgcag aagatgcctc ctcttattca attaaagcga
    28501 tactctcaaa aacatatacc tctggggata aaaaactgta tgttgaagaa aagaattttg
    28561 ataaaatagc agaaatcctg cataagaatc tttctataga caaaaaagat gcactgaata
    28621 ttttagagga cggcgcaaaa aaagagctgt atcaagttga atttggtagc tatgggaaaa
    28681 atatctccca agaaaccaag caaaacatcg aagcggacat gaaaaaagaa ggtgtcgctg
    28741 gtctttattt tgtcgaccat caggctcgga tgtatccaaa tggcgtcttt tcttctcatt
    28801 ttattggata cgcagttcca gacaaagatg agaatggctt agttggtaaa ctaggtttgg
    28861 agtctgctta caatgatatc ttgagcggta aagatggtaa gattatttat caaaaagata
    28921 attttcaaaa tcctctgcca ggtacagtgg cagaagaaga aaaagctgtc gacggacaag
    28981 atatctacac gactttagac agcagactac aaagttattt agaaacactg atggatcaag
    29041 taaatgaaga atatcagcca gaagaattga cagcagtcct gatgaaagca aaaaccggag
    29101 aaatcttggc aatgggacaa cgacctactt tcaatcctga aacaatggaa ggattgactg
    29161 gaaaagatgc tatctggcgt aatttcttgg tacaagatag ttatgaacca ggatcaacca
    29221 tgaaagtttt cactactgca gctgctatcg aagaaggaga attcaacgag aatgaaacct
    29281 tccaatctgg aaaaatccaa gtagaagatg ctacgatcaa tgaccatgac tttggtgaaa
    29341 aaggtgtgct gaccatgcgt caagctcttt catggtcaag taacgtcggg atggtcatac
    29401 tggaacagcg attaggcgga cgatggtata actatttaca aaaactggga tttggacaaa
    29461 gtactcattc tggattagat gatgaagtaa acggtgcttt acctacatta aatattgtcg
    29521 accgagcaat gagtgcttat ggacaagcgg taggtgtgac gaatttccaa atgatgaaag
    29581 ctttcacttc tattgcgaat aacggtacaa tgattcaacc tcgatatatc agcaaagtgg
    29641 tggatcccca aactggagaa gagcgaacaa ctcaaacaga agttctaggg caaccttttt
    29701 caaaagaaac aacagaaaaa gtccgtgaat atatgagaga tgttgtcgaa agcgaaaact
    29761 acggtagtgc ttatggtgta tatagtgtac ctggatacaa tgtatcggca aaaaacggaa
    29821 cagcccaaat cgcttctgat acagggggat atcaaacagg agatacagcc tacctgtatt
    29881 cgattgtgga gatggttcct tctgaagatc cagactatgt cttgtatcta acaatgaaac
    29941 accctaagac atatgatcga atggcattgg ctaaaatcgc aaatccattg atgaaacggg
    30001 caatggattt taaagaaact gaagaagatt cagatacaga aacaaaaaca gaaaaagtat
    30061 ccgtagccga ttatcgtaat cttgaagcgg acgttgcagc agctgatgct caaaaaagtg
    30121 gactgcaacc tgtagtgatc ggtaatggta aaaaagtaca aaaacaatct actgccaacg
    30181 gtgatcagtt gatctctgga gaaaaactga tcctatatac tggtggtgat aagctgatgc
    30241 ctgacgtaac aggatggtca aaagcagata tcatgaagct ggggaaaatc ctcggtattg
    30301 aagttagttt tgatggagat ggttattgtg tcaaacaaga acttgctcca tatgaaaaaa
    30361 tcactgaaga caaacttagt tttactttag aagaataaga agagaaatag gagagaaaag
    30421 agcatggaat ggacacaagc attgattccc attgtcagca gttgtgcaat gacaattgcc
    30481 gcaatgccgc tatttattgg ttattttcaa atgaaaaagc aaggtcaggc gattcgtgag
    30541 gagggaccaa agtggcacaa tagcaaagcc ggaactccta caatgggcgg cttagtgttt
    30601 ctgatcggct ctattttgac aggaatttgg gttggagctt ggcaaaaaca gctaactcct
    30661 actcttttca tcttgctttt tgtattagcg ttatacggag taatcggctt tcttgatgat
    30721 tttataaaaa tattcaagaa aagaaacatg ggactgaact cgaagcagaa attgattgga
    30781 cagattctag ggggaatcat cttttatctt gtttaccgtt ctgaaggtta tccaggtgta
    30841 ttgaatttct tcggactaga tctaccttta ggaatcattt atggtatttt cgctctcttc
    30901 tggcttgttg gattttcaaa tgcagtaaat ctgacagatg gcatcgatgg attagttgct
    30961 ggcttaggaa gtatctcttt tgcagcttac ggtctaattg cttggcatca gcatcagtat
    31021 gatgtattaa tcatttgctt aagtgtttta ggaggtttac tgggtttctt cgtctacaac
    31081 agaaaacctg ccaagatttt tatgggagat gtcggttcat tagcgttggg cggactttta
    31141 gcggcaattt ctataatgct aaaccaagaa tggacattgc ttttgattgg tttgatctat
    31201 gttatggaaa cagcaagtgt gatgcttcaa gttacttcct ttaagcttac aggaaaacga
    31261 atttttaaaa tgtctccaat tcatcatcac tttgaaatgt gcggatggtc ggagtggaag
    31321 attgatacag ttttttggtt gatcagcatt gtgacttcgt tgatcacatt atggatcgtt
    31381 tggtaggaga tgacgggaaa tgaaaaaaat atcaacatat gaaaataaga aagtgcttgt
    31441 cttaggactc gcaaaaagcg gagtaagtgc ggccaaatta ttacatgaat tgggcgcact
    31501 tgtcaccgtt aatgatggaa aaccttttga cgaaaatccg gaagcacaag agctgctttc
    31561 tttagggata aaagtaataa caggtagtca tccaatcgaa ttgttggatg aagaattttc
    31621 attgatggtc aaaaatccag ggattcccta cagtcatcct ttagtagcaa aagcccaaga
    31681 aatgggtatt ccggtaatta cggaagttga gctagcttac gaagtagcgg aatgcccaat
    31741 tattgggatt acaggaacga acggaaaaac aacgacaaca acgatgacag gactactact
    31801 gaatgcagga gcagatcaag gaattgcacg tttagcgggt aatattgggt atccagcaag
    31861 tggtgtagcg caagaagcaa aatcagaaga taaaattgtg atggaattgt ctagcttcca
    31921 actcatggga atcacagatt tccgccctca tatagcggtg attaccaata tctatgaagc
    31981 acatattgat tatcatggta caagaaaaga atatgtcaaa gctaaatgga accttcaaaa
    32041 aaacatgaca gaaaaagatt atctgatcct taactggaat cagtcagaac tacaagaatt
    32101 agcgcaaaga acaaaagcac gcgtactgcc tttctcgaca aaagaagtgt tggaagacgg
    32161 tgtgtatgct gacgattact caatatacta taaaaaagaa aaaataatgg aaatatctga
    32221 acttggcgta ccaggtaagc ataatgtaga aaatgcacta gctgctattt ctgtagcaaa
    32281 attgtatggt atatcaaatg aagctatacg agaaacgttg cattttttcc atggcgtacc
    32341 acatcgtacg caatatgtag gagaaatcca aggcagaaaa ttttataatg attcaaaagc
    32401 aaccaatatt ttagcgacta aaatggctct aagtggattt gaaacgtcta aggtcgtgtt
    32461 attggctggc ggattagaca gaggaaatac atttgatgaa ttaattcctt cattaaaagg
    32521 aatcaaagca atggtcgtat ttggccaaac aaaagaaaag ttgatggatg ctggaaaaaa
    32581 agcaggcatc gagacaatag taacagccga ttctgtagaa caagcagttc ctttagcatt
    32641 agaaaacagt acagatggtg atgtagtttt attatcacca gcgaatgcca gctgggatca
    32701 atacccaaat tttgaaacta gaggaaacag attcatggaa gctgtgaacc gattaaaata
    32761 gaaaaagtag aagcgagcga taaaaaatga aaattttggt tacaggcgga ggaacagggg
    32821 gacatattta tccagctctt gcctttgtca attatgtaaa gactcaagaa ccgaatgctg
    32881 aattcatgta tgtgggagca aaacgaggtc tagaaaacaa aatcgtccct gatacaggaa
    32941 tgcctttcca taccttggag attcaaggat ttaaacgaaa aatttccatg cataacatca
    33001 agacggtccg attgtttctt aagagtatcc gtcaagcaaa aaaaatcctc agtgattttc
    33061 aaccagatat cgttatcggt actggaggat atgtttccgg agcggtagtg tacgctgcag
    33121 ccaaaatggg tattccaaca gttatccatg aacaaaacag tgtaccaggt gttaccaata
    33181 aatttttaag ccgatatgtg gatcggatcg ctctttcatt taaagatgcg gcttcctttt
    33241 ttcctgaaaa caaagcagtt cttgtaggta atcctcgagc acaagaagta gcagatacta
    33301 aaaaatcaga tattttgaaa acatttgatt tggatccaga aaagaaaaca gtcttgattt
    33361 ttggcggtag tcaaggagca cttaaaatca atcaagcagt atcagttttt ttaagtgaat
    33421 atattccttc ttcttaccaa gttttgtatg cttcaggaga tagatattat gaagagatcc
    33481 taaataaaat aaatcctgaa atacaaaata ttagtgtcca gccttatatc aaaaatatgg
    33541 cagaggtcat ggcttccagt gatctgctgg tggggcgtgc tggtgcaact tcgatcgcag
    33601 aattcacagc tctggggtta ccggcagtgt tggttcccag cccatatgtt acgaatgatc
    33661 atcagactaa aaatgcaatg agtcttgttc acgcaggagc agcaaaaatg attgcagata
    33721 atgaactaac aggtgaatca ctaagtcaga cagtcaacga aatcatgggt gatgaagaat
    33781 tgcaaaagca aatgtgccga gcttctaaag aacaaggaat ccctgatgct tcaaaacgtc
    33841 tttatgactt agtaaaacaa ataatcaaaa aataaaggcg gtggtcttag tgagtaagaa
    33901 aaaagaacca aaaaaattgt ctcatccgtc ttctgatgaa aacaatttga ctccttggca
    33961 aaaggctaac cgaaaatatc tggcagaaca agagatgaaa aaccaagagc caaaaaaaga
    34021 agacataaca ttagatacac ccctttcaga ttctgaagaa gaaaaaacgg ttcaagagga
    34081 acagcaagaa gaaagccagt ccgagaaaaa aatcgaattt tttgaagaga tcaagcaaaa
    34141 gaaaaacggt ggaccatata acggttcttt tttgaaccgg ctgcccaatt taaaaagtca
    34201 acggcacaaa gtactatttc gtcggttagc tttgatcatc actgtattgg gtataccgct
    34261 gatttttttg atctattatg tttctcctta tagcaaatta caagcagttg cagtatcagg
    34321 caataagaca gtcaacagcc aagaaatcat ctctgataca aaattgtcat taggagaaaa
    34381 tgtttgggga cagtattttc atcgatctac ttatatcgat cgccttaaaa aagcacagcc
    34441 aagaatcgaa acagcaaata tacatttcaa agggatgaat gaattcgatt tggatgtaac
    34501 agaatataaa gagattgctt tgatcgcaaa aaacaatcaa tactacccag tgattgaaaa
    34561 tggaacagta ttagatgaaa aagtagcaaa tcctacaaaa aatctcccta tcttggaaga
    34621 gttcaaagac tctgcaaaaa tcaaagaatt aacaaagcaa tacaatcagt tatcatcaga
    34681 attacaaaaa gcaatttctg aaatcaagta cacgccaaga gcaagcaata aaaatctgat
    34741 ccaattgaat atgaatgacg gcaatcaagt tattgtaaat atcaataatc ttgcgaacca
    34801 aatgaaatat tattcgcaag tagccaaaga tatggatgaa aaaggtgtga tcgacatgga
    34861 agttggtatc ttctcgcatc cttacggtag cactcaagaa acggaagaat cgggccaatc
    34921 ggaagaaaca actgaaagtg aagaaaatgc ggatcaaaca aacgaatcaa gtgaagaggc
    34981 agaaacgcaa aaagcacaaa cacctggatc gtctagcaca aacggatcat ctgattcaaa
    35041 tgattcaagt gaaacaagtc aaatagaaga taatccaagt acaactccgt caagtttaaa
    35101 tgaaaattga taaaacaact ttaaaaagta agaatgtact ttctaaaaac cagtaattta
    35161 tggtagaatt ggaagcagtg aatatatcta ttaataagta tattttaaaa cggacgctag
    35221 ttgttcgctt gtatatggaa tagtaagtag gagggaatcc cattcatggc aaaaacagga
    35281 atgtatgtag gccttgatat tggaacgaca tctgttaaag ttgtcgtggc tgaatatatc
    35341 gacagccaaa tgaatattat tggagtagga aacgcaaaat cagaagggat taaccgaggc
    35401 attatcgttg atatcgataa aacggtccaa gcgatacaac gagcagtacg acaagcagaa
    35461 gaaaaagcag gtatccagat caaaggcgta agtgtcggtt tgccagcaaa catgcttgaa
    35521 gtagaaaatt gccagggaat gattgcagtt aacggagatt caaaggaaat caccgatgaa
    35581 gatgtcagaa atgtggcttc agctgccctt gttcgttcaa tacctcctga acgtcaaatc
    35641 gtgtcaatat tgccacaaga ttttactgtc gatgggttcg agggaatcaa agatcctcgt
    35701 ggaatgatcg gcgtaagatt agaaatgtac ggtctgttgt ttacaggacc taaaacgatc
    35761 atccataaca tacgtaaatg cgtggaaaat gccggattaa tcgtcaacga aatggtgatt
    35821 actcctttag cgctgactga atcaattctt tctgacggag aaaaagattt tggtacgatc
    35881 gtcattgata tgggtggcgg ccaaacaaca acggctgtta tgcatgataa gcaattgaag
    35941 tttaccaatc ttgaccaaga aggtggcgaa tttgtcacta aagatatctc tattgtatta
    36001 aatacgtcat ttaacaatgc agaagcgttg aaaataaact acggggatgc atacccagaa
    36061 cggacatcac cagatgaaga atttccagta gatgtgattg gtcagtcaga accagtgaaa
    36121 gtcgatgaac gctacttgtc agaaattatt tcagctcgaa tggaacagat tttcaacaaa
    36181 gcaaaagatg cattggacca aatcgaagca ttagagttgc caggtggcgt cgtattgaca
    36241 ggcggagctg ctagtcttcc tggtgttgtg gacttggcac aagaaatctt cggcgtaaat
    36301 gtaaaattgt atgttccaaa tcacatgggg ctgagaaacc cggtatttac aaatgtaata
    36361 agtatcgtgg attattcagc aaacttgagt gaagtgtatc aattggcaaa aattgctgtt
    36421 acgggtgaaa cctcagcagc tcgtcaaatg gtagttgaac aagaaacaac aaacacatat
    36481 gagagctatg aagcacctga agaaacaatc tacgatgagc cggaaccaaa agagtccgga
    36541 gaaaatgtaa aaaataaaat caagggtttc tttacaaata tttttgatta attcatcctg
    36601 ggaggaataa gatatggaat tctcaattga taataatatc aatgacggcg cagtaatcaa
    36661 agtcatcgga gtcggtggtg gcggtggaaa tgctgtaaac cgaatgattg aagaaaacgt
    36721 taaaggtgtt gaattcatta cagccaacac agacgtgcaa gcattaaaaa attcaaaagc
    36781 tgaaacagtt atccaattag gccccaaata tactcgtggt ttaggtgctg gttctcagcc
    36841 agaagttgga caaaaagcgg cagaagaaag tgaacaatcc ttacgcgaag ctttagacgg
    36901 tgcagatatg attttcatca ctgcaggaat gggtggagga accggtacag gtgctgcacc
    36961 aatcgttgca ggtatcgcga aagaattggg cgcattgact gttggtgtcg tgacacgtcc
    37021 attcactttt gaaggaccga aacgtggccg ttttgctgca gaaggtatcg ctcgtttgaa
    37081 agaaaatgtg gatacattat taattatctc aaataaccgt ttattagaag ttgtagataa
    37141 aaagacaccg atgcttgaag ctttccgtga agcagataat gttttacgac aaggtgtaca
    37201 aggtatctct gatttgatca cagcgccagg ttacgttaac cttgactttg ctgacgtgaa
    37261 aacagtgatg gaaaaccaag gtacagcatt gatggggatc ggtgtggcta gtggagaaga
    37321 tcgcgtagta gaagcaacga aaaaagcaat ctcctctcca ttgcttgaaa catctattga
    37381 tggagctgaa caagtacttc ttaacatcac aggcggtttg gatatgacat tgttcgaagc
    37441 acaagatgct tcagatatcg tagcaaatgc agcaactggc gatgtaaata tcattctagg
    37501 tacatctatc aatgaagaaa tgggcgatga aatccgtgtg acggtcatcg caacaggtat
    37561 cgatgaatca aaaaaggaac gcaagtctag ccgaccagct agacaagcac aaatgcaatc
    37621 acctgctcaa aagactgtat tagatatgga tcaagctaaa ccaacttctt cagaagaaga
    37681 aaacagtttt ggcgattggg atatcagacg tgaacaaaat gttcgtccta gagttgatga
    37741 ttccaatttt gacaatatcg aaaaaaaaga atttgataca ttcaaccgcg aagaaacaaa
    37801 atctcatggc gatgatgaat taaatacgcc accgtttttc cgtcgtaaaa aataaaggag
    37861 aggaagagca catgattgct gacaacttgc agaaaatcaa gcaagaaatt caggattcgt
    37921 gtgctcttgt tcatcgtgat ccgaaagaag ttacattagt agctgtaaca aaatctgttg
    37981 aatccgctca agctgaagaa ttggttagag ctggcgttat gaattgtgca gaaaaccgag
    38041 tagataaatt attagaaaag aaacaagatt tatttgattt tcctgaaata aagtggcatt
    38101 taattggtaa tttacagcgc agaaaggtaa aattgattgt aaatgaaatt gattactttc
    38161 atgcgttgga cagtttaaga ttagcagaag aaatccagaa gcggttagag aaagaattat
    38221 cctgttttat tgaagtaaat gtttctggtg aggaatcaaa acatggaatt tcaccagatg
    38281 agcttttacc gtttgtggaa tctttagctg cctgcacaaa aatcaaggtt gttgggttga
    38341 tgactatggc gcctaaagaa gcttctgatg agcaaatcca taaagtgttt ggtacgctga
    38401 aagaattgcg tgatcttgtt caagcaaaag gttatgccca tgcaccatgt agtgaattaa
    38461 gtatggggat gagtcaagac tttcaaattg cgattgaaga aggtgcaaca tttatccgtg
    38521 taggaacagc gctattcaaa gacgaataaa ggaggaacaa aaatgccgct tttaaacaga
    38581 ggagcaattg ctaatttttt cggattagct gatgaggaag agtacgaata ccaagattat
    38641 cctgaaacaa caaaaaaggc tgtacagcca actaaaaaaa atacaacaca gaatcaatca
    38701 actcattctg tatctgctgc gaatactcat gaaaaacaaa aaacggcgac ttcacgtcct
    38761 gtaacgcatg aaagtattca gccggctgaa aagaaaaaag taattgaact acatcaatca
    38821 cctacaaaaa ctgtagcgaa agaaagaaca gcttcaagaa cctctacaca acctaaagca
    38881 aatcttgctg gaaaaatcac tgtaatggaa ccaagagctt attctgaagc tatggcgatt
    38941 gctaaacgaa tcattgctgg agaagcaacc ttagtgaatt ttcatttagt agaagaaaaa
    39001 caagcgagaa gaatcgttga ttttctaaca ggaacagttt atgcactaga tggagatatc
    39061 aaacgtgttg gagatgagat ttttctttgt acaccgactg ggatggagat tgacagttca
    39121 accgctcaat cttttgcaga tgcgaatttg tttgatctgt aagaggagga actacgattt
    39181 atactattgc actattactt catttacttc aacgggcagc ctatttctat agcattatct
    39241 tgatggtata tgcattgctc tcttggtttc caggcgggta tcaatcaggg ctaggacgat
    39301 tattagctaa gatctgcgaa ccgtatatca gtttgttcga tcgtctgcct ttgaggattg
    39361 gacccttgga tttcacaatt ttaggggcaa tcctgatctt gaacttagcc gtacaaggtt
    39421 tagcacagat cgttatatgg ataactgtcc atttagttta ttgattgaag caacaaaatc
    39481 tttattctca gaatagatag gaggggaaaa ttttggatgc aaatgtatac cagcactttc
    39541 gggcagatga acgtacattt atcgattcgg taggcgattg gattgaacag gttcaaggac
    39601 aatatgcacc ctatctgacc gaatttcttg acccaagaca atcctatatt ttagaaacac
    39661 tgattcgaca aagcagtgat ttgcgtttca tgttttatgg cggatatgaa caggcagaaa
    39721 gaaaaagatg tttgattttc cccgattatt acgaacctgt agaggaagat tttgaaacag
    39781 cattgtatga aatccattat cctagcaaat ttgccagcct aagtcatggg aaaatccttg
    39841 gaactttagt gggaacgggg atcaaacgct cttactttgg cgatatcatc tcggacggtg
    39901 atcgctggca agtatttatt gcaaatgagg tggatcattt tgttgctgca caagtaacaa
    39961 agattggaaa ggttgctgta cgcttggaac caatcaaata cacagaaatg attatgccga
    40021 aagatagctg gacacaagaa agaggaacag ttagttccat gcgactagac agtgtgatct
    40081 ctgagattta taatatttcc agacagcggt ccaagcagct gatcgaagct ggtaaagtaa
    40141 aagtgaactg gacagaaaat acaagacctg attttgtact tgaactatta gatattattt
    40201 cagtcagagg ttatggcaga gtccagatcc aagaacttga ggggaagacg aaaaaagaaa
    40261 agtacagggt actgttcggt gtattacgta aataaaataa aaaggtggtt tactcatggc
    40321 tttaactcca ttagatatcc aaaataagac attcccgaca aaaatgcggg gatataatca
    40381 agacgaagta gatgatttct tagatttagt tgttcgtgac tatgaggaat tgactcaaag
    40441 aaatcgagaa ttagaaaaag cagtcaaaca ttctgaagaa aaacttgagt acttcaatga
    40501 attaaaagat gctttgaacc aatcaatcat cgtagctcaa gatacggctg ataaagtaaa
    40561 aacaagtgca agcaaggaat ccgaagtgat cgttacttct gcacaaaaca aagcagatga
    40621 gttagttgcg aacgctgaaa aacgtgcaca tcaattaaca actgatgcag aagaaaaagc
    40681 aagaaaaatc ttgacagatg caacagaaaa agcaagacag cttgcgacag aaactgaaga
    40741 tcttaagaaa aaaacacgag tattccatca acgtatcagc ttgatgcttg agtctcaatt
    40801 agaacaagtg aaaagtccgg aatgggatga aattttacaa ccattctcca gctacgtgac
    40861 tgacagccat gaagtgatca aagaagtttt agcaaaacag cttgacaatg aaaatgatac
    40921 tgatgtaaac tcagacacag gagtcataga agcacctatg acggcatttg atacacaggc
    40981 tattgatatc tcgatcgtgc ctggtgaaaa tgaaaacgag tagaacagaa aagcactttg
    41041 ttattcttaa agcgagccga tgagagtgga agttcggtaa gacactgcat ggtgaaagat
    41101 cctctacttg agacctgctt ttccgaaatt agagtaagaa gcagcggctg acctcgttat
    41161 tggtcacaga gagtttgcac tagatagatg caactaaatt tgggtggtac cacggcatgt
    41221 tcgtcccttg cggctgaaca tgcttttttg tgtccggaaa acaaataaag gagtgtatgg
    41281 ttgaatgaaa atgaaagaaa ccttgcaatt aggaaaaaca aaattcccta tgcgtggtaa
    41341 tttgccaaac cgagaaggcg agtggcaaaa agaatgggaa gaaaatcgaa tctaccaaaa
    41401 aagacaagag ttgaacgaag gaaaacctag ctttgttctt catgatggac ctccatatgc
    41461 caatggtaat atccatttag gacattcatt gaataaaatc agtaaagaca ttatcattcg
    41521 ttcaaaatca atgtctggct atcgtgcacc atatgttcca ggatgggata cacatggatt
    41581 gccgattgaa caagtattgg ctaataaagg cgtgaaacgt aaagaaatgt ccatggcaga
    41641 atacttggaa aaatgtcgtg aatatgcact ttcccaagta gataagcaac gagcagattt
    41701 caaacgccta ggagttgcag gagattggga acatccttat gttacattag atccttctta
    41761 tgaagctgca caagtacgcg tatttggtaa aatggctgaa aaaggctata tttacaaagg
    41821 attgaaacca atttactggt ctccgtcaag tgagtcttct ttagcggaag ctgaaattga
    41881 atataaagat gtaaaatctc catctatttt tgtagctttc aatgtcaaag acggcaaaga
    41941 tattcttgat gaagatacag cttttgtcat ttggacgaca acaccatgga cattgccagc
    42001 aaaccaaggt attgctgtaa atccaaaatt tacgtatgta ttggtggaag ccgatggccg
    42061 taagtttgtc gtagcgaaag atttgctaga aacagtccaa tcagaaatcg gttgggaaaa
    42121 tgtaacagtt ctaaaagaat ttgctggaca agaaatggaa tatatgactg caagtcatcc
    42181 attctatgaa cgtgaatcac ttgtgatttt aggcgatcat gttactttag acgctggtac
    42241 tggattagtc catactgctc caggacatgg ggaagatgac tatatcgctg gtagcaaata
    42301 taaactatct gtagtgtcac ctgtagactc aaaaggattg tttacagaag aagcacctgg
    42361 atttgaaggt atcttttatg acaaagcaaa tcctatgatc actgatctat taaaagaaaa
    42421 aggagctttg ctgaagctag atttctttac tcatagttat cctcacgatt ggcggacgaa
    42481 aaagcctgtg atctatcggg caactccaca atggtttgca tctatcgata aattccgtca
    42541 gaatattttg gatgaagtag aaaaagtaga gtgggttatc ccatggggta aaacacgttt
    42601 gtataatatg atccgtgacc gcggcgattg ggtaatttcc cgtcagcgtg catggggagt
    42661 accacttccg attttctatg ctgaaaatgg agaagcaatc atcacaccag aaacaatcga
    42721 acatgtggca aaattatttg ctgaacatgg atcaattatc tggtttgaaa aagaagcgaa
    42781 agaacttctt cctgaaggat ttacccaccc aggttctcct aatggcgaat ttacaaaaga
    42841 aaaagatatc atggatgtct ggtttgattc tggttcttct catgaagctg ttttacgtca
    42901 gcgaccagaa ttatcattcc cagcagatat gtatttagaa ggatcagacc aatatcgtgg
    42961 ctggttcaac tcaagtatca cgactagtgt ggctatcaat ggtgtagcac catacaaatc
    43021 agttatttct caaggatttg ctttagatgg tgaaggccgt aagatgagta aatctttagg
    43081 aaacatcatt gcaccagaaa aagtgatcaa acaatttggt gcagatatcc ttcgcttatg
    43141 ggtatctagt gtagattatg aagcagatgt tcgtgtttct atggatatct taagtcaagt
    43201 ggcagaagta tatcgtaaaa tcagaaatac gatgcgtttc ttattagcga atacagaaga
    43261 tttcgatcct aaaaaagata ctgtttcgta caatgattta cgttctgtag acaaatatat
    43321 gaccgttcga ttgaaccaag taatcaaaga aatccgagaa gaagggtacg acaaatataa
    43381 cttcatgcat atttaccgta cagttatgaa cttcttgacg gttgatctat cgtcattcta
    43441 cctggatttt gctaaagatg tagtttatat tgaagcagaa gatgattatc aacgtcgttg
    43501 catgcaaact gttttctatc aaacagctgt agctttgaca aaattattga ctcctatcat
    43561 tccgcataca gcagaagaaa tctggtcatt cctgaaagaa gaagaggagt acgttcagtt
    43621 aagcgagttt cctggttatg aagagtttgc aaaccaagaa gaattgatgg atacctggac
    43681 tgctttcatg gacttccgtg ataatgtctt aaaagcattg gaagaagcaa gaaacagcaa
    43741 attgattggt aaatctttgg aagcaaaact aactgtttat ccaaaacaac aagttcgtga
    43801 aatgttaaaa gctttggatg cagatattgc acaattattg atcgtttcac cagactactt
    43861 tgaagttatg gcagcagatg aacaagtgcc agataacgct atggtatttg atgatgttgc
    43921 tattacagtg gaaaaagcag acggggaaac ttgcgatcgc tgtcgccaag ttcgtaaaga
    43981 tgtaggagta gatgaaaaac ttcctcatct atgcggtcgt tgtgcaaaaa tcgttgaaga
    44041 cttctatcct gaagctgtag ctgaaggatt cgaagaaaag taaaagggaa aagggggtgt
    44101 gacatgttct ttgtcacacc cccttttgtt atcaatcgtt taattttcca cgttcacgat
    44161 accagttatc tggttgccag atccattcga atccgtcttt ttcaagtagt tcgaaagctg
    44221 ctttaggtcc cattgatcca gctttgtagt tgggaaaatc cggttgtgtt tggtcccaag
    44281 cttttcgaat gacatccacg atatgccaag attgagcgac ttcttcccaa tgagagaagt
    44341 tcgttccatc accattcaac gcatctaata gtaatttttc gtaagcttct gggctatttt
    44401 caaccatttc tgcactattt ctaaattcca atttgattgg ttccgtttca aatccttgcc
    44461 ctacttcttt cccattcaag gataaagaaa atccttcagt tggctgaata tagatcgtta
    44521 gaacattagg tggaagtgtc gtatcgtcac atggttcatc gacagatgtt ttgaatacat
    44581 taacaggaac ttgtttgaat acgatgttga tccgagtccc tttttcagtc aaacgtttgc
    44641 ctgtacgtac atagaaagga acacctgacc aacggaagtt atcgatagtg aatttgcctg
    44701 cgacgaatgt ttcagtctct gattgagcat ccactttgtc ttcttcgcga tagcccttga
    44761 attgtacacc atccaattca ccttgagcat attgcccgcg aacgaagttt tctaacactt
    44821 cttcttcgtt ataaatgcgg atagctttca atgctttgat cttttctgta cggatttctt
    44881 tttcggaaaa tgctactggc ggttccattg caagtaatgc tacaacctgt aaaatatgat
    44941 tttgcaccat gtctttaagt gcgccactat gatcgtagta gcccccgcga tcttctacac
    45001 cgaggttttc gccaaaagta atttggatat tgtcgatata gcgattgttc cattgagcct
    45061 caaaaatatt attggcaaaa cggatagctg agatgttttg gatcatttct tttcctaaat
    45121 aatgatcaat cctgaaaata tcttgttctg ggaatacttc tctgatttgc tggttcaatt
    45181 caaaagcaga ctggtaatca gaaccgaacg gtttttctat gatcaaacga ttgaagccat
    45241 tttttgtcat aatattttgt gatttcaaat gagaaacgat cgttccaaaa aattgaggtg
    45301 ccatagccaa ataatagaca cggttaccgt ttaattgata gtgtccatct aatcggtcag
    45361 acaattcttt taatgtctga taatgcgttg aatcatttac atcatgggat tgatagtaaa
    45421 aatggcttgc aaaggtagtt gcttcttcat ctgaaggatt caatgcacgg attgtttctt
    45481 tgacaacttc acggtagtgc tcatcgctcc agggacggcg agcggttccg attacagcaa
    45541 aatgctcgcc taaatttcct tttctgtaaa ggcggaacaa agacgggtac aatttacgct
    45601 gggctaggtc acctgtaccg ccgaatatcg taaacaatac atttttttca ttagacatgt
    45661 ggatacactc ctttatattt tatcgacaaa aatcgtgctg gccgccttaa agctgacggg
    45721 gattttcttt ctatcatttt ggatagtgat cggtccttca taagctgcga tgtcaacgat
    45781 ctcatattct tcgttgatat tcaggtcaat agtgactaag taatccaaca attctttttc
    45841 atcaagcact cgtgcgatac gcacttttgt accgacagga taggactcca atgtttgtcg
    45901 gcgttcttca tggacaactt cctctttttt aggaatcgat cctccatgag gacaatgttc
    45961 aggatgattc aaatagtttt ctaaatgatc tgcaagatgt tccgaagtca catgttccaa
    46021 tacttcagca tcatcatgga cttcattcca gctatagtct aaatgttcaa ctaaaaaaac
    46081 ttcccataag cgatgtttgc gaatcagact gctagctttt tgcagtccgc ttgcagtcaa
    46141 ttgtacacct tgataagggg aatgttctac aagctcttct ttcacaagtt tagaaatcat
    46201 ttcactgaca gatgcagctg agacatctag actagatacg atttgtttat tgttgacttt
    46261 cgttgtatct ccacctagtt ctaaaatgat tttcaaataa tcttctcgat ttggtgtcat
    46321 attcgatcat cctttcatgg taaaaaacca tcattagtaa gtgtaacaaa aaaaacgtta
    46381 attgactatt tttctaaata aaaaacaaag gatttttaga cgtttttaca acaaaaaacg
    46441 aaagatgata gagaagactc attctttcgt ttataaagga tatatatata cattgggagg
    46501 gatctatttt tcagataaca acttatatga agtgtctgca gattcgtcat aaagagtttg
    46561 ttcgggataa gaaaagataa gataaaaacc atactgatca cgtacgacta aacgatattc
    46621 atctatttct accacagatg tgccaagaga ctgaccgttg atcaaaaacg tgtaatcaga
    46681 tgtaatctgt aattgttgtt tttgctggct atcactaaag aaccatgtac cttgatactt
    46741 tgagagtcct tctgactttt ccgcttgttt tttctttttc aagtaaataa aacttgagag
    46801 aacggtaatg agtgaaccaa gaaaatagat catttttttt atcaccaaat cactccttta
    46861 tgagaaaata atcgcataga catattcttt cttctttatt tcattgtacc gaataaaagc
    46921 taaaatttct ctaagacttt cgactggttt ttttactgtt tttctacaaa ttatgatagg
    46981 atacatagta ggaagaaagg tgacataatt tgaagtatca gcaagtggaa atagcttttt
    47041 ttattgaccg cccgaatcgg tttatagcac attgtatgaa aaaagacgga gaaataatta
    47101 ttgcgcatgt caaaaataca ggtagaggaa aagaggtcct gataccaggc gctgaagtgg
    47161 ctattgtatt tgctcctgga cctaaacgga aaaccgccta tgatctgatt gctgtaaaaa
    47221 agaagggtga ctggtttaat atcgatagtc aattgcctaa tcggctggca attgatggta
    47281 ttttagacgg cacgatcctt ttaccaggat taactggtcc atttacggta tacaagcgtg
    47341 aagtaactta tcttcactcc aaatttgata tttatttaga gacggtggtc ggacaaaaag
    47401 catttgttga agtcaaaggt atgacacttg aaaataaagc gattggtgct tttcctgatg
    47461 cgcccacact cagaggattg aaacatatag gagaattaac gtatgccgca caagatggtt
    47521 atgccgctta tgtcttgttt attgctcagt tcgaacatct tcatctggca acgatccatg
    47581 aagaaatgca accagcacta gctgatatgg ttcgacatgc gcagcaaagc ggagttcaaa
    47641 tcctcgccta taattgtcaa gtatcggcag atgaggtaac tgtcaagcag gccattccat
    47701 ttgatgtaga tatccctttt gaagatccta tacaatagaa agaaggtaaa ataatgatca
    47761 acttaggtat tatcggaaca aattggatta ctcatcaatt tgtccaagca gctttaagca
    47821 caaaaaaata tgaattgaca gcggtatatt ctagacattt ggaaaaagca caggaatttg
    47881 gttctcatta tgaaggggat atcgaattcg caacagattt gaagactttt ttcaatatta
    47941 ctcatatgga caccatttat attgcttctc cgaatagtct tcactttagt caagctaagc
    48001 aagcaatttt ggcaggaaaa aatgtgatag ttgaaaagcc agctttttcg acgccaaaag
    48061 aaatggatga aatcatccat ttggcaaatg aacacaatgt gtattttttt gaagcagcaa
    48121 gaaatatcca tgaatcaggt tttaaaaaga ttgccgatta cttaccatta aaagacgaga
    48181 taatcggtgc aaatttcagc tatatgaaat attcttcgcg ctatgaccaa gtattagatg
    48241 gagaagaacc gaatatcttc tcgactcatt tctcaggtgg tgcattgatg gatttaggtg
    48301 tttaccttgt atatgcagct gttgcttggt ttggaatgcc aaaagaagtc cattatttcc
    48361 caagaaagat ttctactggt gtagatggtc ttggatgggg gatcttacgt tatgagaatt
    48421 ttgatgtatc gatccagcca ggtaaaatcg gtgattctta tttaccgtca gagatttatt
    48481 ttgactcagg aacgctagtg atgaatggag tcaatgcaat tgaacaagca caatttcatg
    48541 atcgcgcgca taaagaaatc attgatttgg cggtaacagc tgcagaaaat ccaatggcag
    48601 atgaagcaga ggattttgcc aatgtcatgg ctcatccgaa agatccagct tggggattgt
    48661 tgtatgaaga gtggatcgag ctagctagaa atgtgaacca agtgatttat gatttaagaa
    48721 aaaatggcgg catcaaattc gatgcagata atgaggaaga tttttaatga acgaatttgt
    48781 acaagtattt cccgaaaatt ggcagaaaaa atatgaggat aaagggttta tctcaccttc
    48841 tcttattcaa cgagcagttt ttcagccact aagtaataaa caaagtatag tagcggtttc
    48901 tcctacaggt tcaggtaaaa cactggctta tctttggcct ctactactaa acgtggaacc
    48961 aggtgaagca agtgcgttgg tcatttttgc ttcttctcaa gaactagcta ttcaagtagc
    49021 agatgttgca cgtgaatggg gaaaagacaa agagttgaaa gtccaatctt tggtgggcgg
    49081 agccaacgtc aaacgtcaga ttgagggttt gaaggaaaaa ccagaaatct tgattggtac
    49141 accaggaaga atattggaat tgatgaaagc aaaaaaaata aaagcacatc aagtgaaaac
    49201 aatggtattt gatgaagcag accagctatt tgacgctggg aacagccaaa tcattgatca
    49261 aattcttcat caagctccaa cagagtatca gctagctttt ttttcagcga cagcagatcg
    49321 ttcccttgaa agcattgaaa aaatcactgg gaaaacgttt gagacaattg acgtgacagc
    49381 agaggacgat tctcgaaaag gactgagaca ttacttttta cgtgtaccga tacgaaagaa
    49441 agatgactac ttgagacgtc tcacgcatat agaagatttt catggtctga ttttctttaa
    49501 tcaactgact gaattaggcg taatggaaga aaaattgctg tatagaggtg taccggtagg
    49561 aagtctggcc tctgatcaga acaagctact ccgaaaagca gcgctagaac aattcaaaaa
    49621 gaaaatggtc agagcacttt tgaccacaga tgtggcggct agaggtttag atattacagg
    49681 tcttccttat gtagtgaatg cagaagttcc gttaagtgaa gaagcttatt tgcatcgttc
    49741 gggaagaact ggtcgaatgg gtaatgaagg aatagtaatt acactcgtac aagataataa
    49801 tctaagagat ttaaaacggc ttttacgtcc atcaaatata acacttgaag agattttttt
    49861 atacggaggt aagctgcaaa ctgagcagcc aaccaaagaa gatgcagctc ctgcagtaaa
    49921 taaaaagtat gcgaaaactt tttctggaaa ggagcagaac ccagaatata acaaagaaaa
    49981 aataagaaaa aaagttaaaa ataaatcaaa aaaagagaga aataaaggag caaggcgcaa
    50041 ataagcgcct ttttatgcta tgattatgaa aacgggaaca aaattggttg ccagaggagg
    50101 ggttatgtga aagaacgtca agaagagatc ttgcagctat tgacagaaaa ccaacaagga
    50161 ttgacagcaa gtgatgtagc tgagcgctta actatcgatc gaagcaatgc aagccgatat
    50221 ttaagtgaac tatacaaggc ccatcatatt gtaaaaaccg ctggccgacc agtcgtttac
    50281 tctttgccaa cggaaaagtc aaaatctgat gaagtgcatg tcgactcttc tacgcaagtg
    50341 acttttgaaa cactcgttgg agaaaatgat tctttaaaag tcagcataca gcaggcaaaa
    50401 gctgcgattc tttatccacc gcgggggttg catacgatta tttttggaga aactggtaca
    50461 gggaagtcga tgtttgctga atgtatgtat cattttgcaa tcgattcaga gatgctttct
    50521 gcagatgcgc catttgtttc tttcaattgt gcggattatg ctcaaaatcc gcaactgctc
    50581 tttggacaca tctttgggat caaaaaaggg gcttacacag gagcagctca agatagtccc
    50641 ggtctaatag ccaaagcaga cggcggtata ttgtttttag atgagatcca ccgtttaccg
    50701 ccggaagggc aggaaatgct gtttactttc attgataaag gtatttaccg tccgcttgga
    50761 gaaagtgcac aagtacatga agcatctgtt cagatcattg gggcgacgac tgaatcttca
    50821 gaaagtttct tatctacgtt caatcgacgg attcctatgg caatcacctt gccgaatctt
    50881 gcttcacgtt cactggatga gcgttatgaa atcatctctc tttttatcaa acaagaagcg
    50941 aatcgtttga atcaacgtat cgatatcgaa aaagaagcga ttcttgcctt catgctttat
    51001 gatgcggaag gaaatatcgg tcaagtgaaa cgagacttga aactagtctg tgcaaaatct
    51061 tttttacatt accgtactca tcatgagaaa aagctaatca ttcgaaaaga agaactttcc
    51121 cttcaagtac aaaaaggatt actgaaaatc aaggaaatac cggaacgtct cgaccgattc
    51181 atggacagca aaagtcagta tttgacattt gaaccaggat ttgctgatgt tgtctggtca
    51241 caagatccag aacgaaacat gcaggtctat aacgatattg aggaaaaagt cacttctctt
    51301 tccgaaaccg gtgtggagaa cattgatcta gagaccctca tttcaaaaga tgtggatgct
    51361 tatttccaga catatgtcaa agaattgact aaaagttcga ttcccaaaga tcttttgcca
    51421 gatgaaatct ggcggttaac caatcaattg tatgatgtgg ctgaaaaacg gttagaccgt
    51481 atttataatg aaaaagctcg ttttgctttt gctttgcatc tgcaaagtac attggatcgt
    51541 gtgagagaag gtcatatgat cgtccaccca gatttgaata acgtacggaa aaattttaag
    51601 agggaattcc aggtagcaat cgatctttca acgatcattg aagaagaaga acaagttgag
    51661 attccttttg atgaaatcgg ctttattagt atgtttcttt ctatcaatct aggagaaagt
    51721 gagcctgtta aagaaaacaa agtagatgtt ctcatattga tgcacggcag acagactgct
    51781 tcaagcatgt tggagatggc acaagaacta ttaggaacaa aagtagggaa agcttttaat
    51841 atgccactgg ttatggaagt tcaaacaatg tatgaagaag tgttacaata tgtcaaaaac
    51901 tatcaagagc atttgacgaa tggcctactc ttgttaacag atatgggatc attaaacacc
    51961 ttcagtgatt tgatttatga agagactggc attcggacga aagctatcac gatgacaagt
    52021 accatgattg tgatagaagc gatacggatg gcaagtgttg gaagaagctt agaagatatt
    52081 tatcaaaata tccagctttc ctttgaatcg atcgtacgtg atcagttcaa agcggaaaag
    52141 tctgaaactt tacagcgtaa aaaagccgta atcgtgacat gctttactgg tgaaggagta
    52201 gctgccaaat tatatcagcg tattatgcca gtgattaatc aaaataaggt agaactgatc
    52261 cagatgcagt ttttagaacg tgaatcattc aaaaaacaca tcgatgcctt attggaagaa
    52321 tatgagatca aagcaattgc gggaacagtt gatgttgatt atcaaaatat cccatttttt
    52381 tcagcctatg acgtttttga tgatgaaaaa ctaaatattt taaagcgtat agctgatgat
    52441 gagatcccga cagctaaaat tgtcgattca cttgctggat cattaagcga tgtcttttct
    52501 attgatgaac tgatccgttc attgcaaaag acagttcagc agatccagac cgatttgtcg
    52561 attatcgtgg aacctggagt agatactgga atcgttatcc atttagcctt catgttagac
    52621 ggattgatca aacaagaaag ccatcggcaa tttgagaaac tgtctatttt ctcaaaaatt
    52681 tatcgtttgg aaatggatat cgttcgggca aacttgatgg cactagaacg aaaatatcag
    52741 gtaaaaattc cagaagatga tatcgcattt cttacacaaa tgtttcttga aaataaaata
    52801 aaaaaatgaa cacactaatc atactgttta cacactaaac atctgtgtgc gcactataaa
    52861 aaagagctaa aaagcatgaa aacgctattt cagctctttt ttttattggc atgcttcttg
    52921 cgttatataa aagtgtaaaa aaataaaagg agtgttaaaa atggcaaaaa aaacaatcat
    52981 gttagtttgt tcagcaggga tgagtacgag tttattggtc acaaaaatgc aaaaagcagc
    53041 agaagaaaaa gggatggaag ccgatatttt tgcggtctct gcctcagatg cagataataa
    53101 tttagaatct aagaatgtcg atgtattatt attaggtcct caagtacgtt tcatgaaagc
    53161 tcaatttgaa caaaaacttg cgcctaaagg tattccatta gatgtcatca atatgcaaga
    53221 ctatggcatg atgaacggag aaaaagttct tgcacaagca gaaaatctaa tgaaataaat
    53281 caagtatgca gttgggggta taacaacgtg gaagatcaac aaaatctaga agccatcatg
    53341 gggttaatca tgtttggcgg taatgcaaag agcgatgcaa tggaagcgat tgcggctgct
    53401 aaaatcggtg attttgagtt agcggatagg aaaatcgctg atgcagaaga atcacttata
    53461 caagcgcatc atgcacaaac agacatgttg acacaagaag cacaaggaaa tcatatgcaa
    53521 gtgacattat tgactgttca cagtcaagat cacttaatga catctattgc gtttactgat
    53581 ttagcaaaag aaatcatcga actttatcgt cgcgtataca gcgaataaat acaacttctt
    53641 ttttgaacgg aagaagaaaa atttttgaac ggatttttta ttttgttata aggagaactc
    53701 tctttatata aaaaacttat aggaggaagt aaaaaatgaa cggactatca agttggatgg
    53761 agaaatacat cctgccggtt gccgcaaaaa tcggagcgca aaaacattta gttgctttgc
    53821 gtgatgcttt tatcggcaca ctgcccgcaa caatggcggg atcagtcgct gtaatgatca
    53881 atgcaattat tcgtgattta ccgcaacagt ttaacagtac ctatgctgac agtttagcag
    53941 cagacaaagg aatctttgcc gtattgaatg tcatcatcgg aatcaatggt tttgtctgga
    54001 acggtacact agcaatcgcc ggattgatct ttgccttttc ttgggggtat aatcttgccc
    54061 gagcttatgg agtaaatgat ctagctgggg ggatcgttgg tttagctacc ttgattcaag
    54121 gtatttcctt tggtccagca ttatcagcag cattagatgt gaatgttcct tcaaatgtcg
    54181 cagatgcaat caatagttct gagattggtg caacgattgc agatggcaca ctgtcagttg
    54241 gattatgggg ttggctaaaa ctagatcatt tgaatggaaa cgcttatttt acagttatga
    54301 tcatgggagc tatttcagtc attatttttt gtaaattgat gcttgccaac attacgatca
    54361 aaatgcctga ttcagtacca ccagcagtat ctaaagcatt tgcagccatt ttaccagcga
    54421 cgatcgcttt atacgtcatt gctattatca attatatcgt aggtcgtgta gctgatgggc
    54481 aactgatcat tgatttggtg caaaaatata ttgcagagcc attcctagga ttatctcaag
    54541 ggttgggagc agtattgatc gtcacagtat ttgttcaagt attctggttc tttggtattc
    54601 atggaccaaa tgtattggca ccagtgctag agggaatctg gggacaagca caactaatca
    54661 atattgatat tttccaaaaa ggttataacg gaaaaactgg tacaccagcc gtgttagatg
    54721 caatcgaaga tggaaaagct tatatgtggg tccgcggatc gttcgatgct tttgcatggt
    54781 ttggcggatc aggcggaacg atcgtcttac tgatagctat cttgattttc tctaaacgtg
    54841 cagattatct aacagtagct aaattatcca ttgggcctgg tatcttcaat atcaacgaac
    54901 cagtgatgtt tggtctgcca atcgtattga atgcaatcat gtttatccca ttcttgattg
    54961 ctccagtagt agcaacagca attggatgga cagctactta tcttggccta gtcgctccgg
    55021 tatcgcagca agtcacatgg gtagtaccgc cgttcttatt gtcattctta gcaacaggag
    55081 cggattggcg ggcaccgatc gtaacattag tatgtatggt cgtgactttc ttgatttggg
    55141 caccatttgt catctctgca aacaaaatgg accctgccgc aggcgaacaa caataagatg
    55201 aacgtgagga gcgggacgct ttaagtttcg ttcctcataa tgaattttcg aggaggaact
    55261 gaaatgatta caattgccag tgcaatgcaa caagatgaaa tcaaagaatt gttagaagga
    55321 tacaatgaag aaggaatgac ttttacattt aaagaaaaac aaggaatcaa gctgttcttt
    55381 gaaacaagtg cagaagatcc ggaagttgcg gctaaaaaag caaaggaact aatcaaagca
    55441 cagccttggg gaacagtcct ttattttcaa tcaacagctg tttagaaaat ggtttaaaaa
    55501 gataggatgg ttgagatgat aaaaaacggt ttatttttat gtagtattgg ttctttgatc
    55561 gtgttgtata gtttgaatcc acattcgcaa aggtatgatt tgtatagtat ggtcacaggt
    55621 ctttttctaa caggattagg aatctatttc ttcatgaagg ggaaaaaaag agaagaagca
    55681 aaggagaaaa ataactaatg gaaatcacga aaaaattaaa tattccagca tcgttttttt
    55741 acgataaagt tatggactct gtccttttcg atgtacgtaa agcgaccggc aaatcagtaa
    55801 ctagaaaaca gctgaaaaat ttggagtacg tcaaacaatt ctcgcaaaat aaccgggctc
    55861 gcataaaaat cgaagaagtc attgaaaacc agtcttataa gtttcgtaca tcgacaacga
    55921 agaatgaatt cgtcgttcat tatcaaatca aaccattgga cgattcgacc tgtgaggttc
    55981 attacacaga aaaaatggag tcttatggca tgcttcaaaa gttgaatgat atgcttttag
    56041 gaacaatctt gatgtatttt aaacggagac gtttcaaaaa aatgctagat atgatcgaag
    56101 caacgtatta aatcaatgaa acatggtact atttagttat tgaaaaatga agtaagctag
    56161 aaggaatttt tcataggaag atagatcttt cctgcgaatc ctcactagct tttttgtagg
    56221 aaggggcagc aaaatgacaa acaaagtaag agagctaggt gtatcttcga ttgaggaaat
    56281 gtttgattta gccacttatg catttaatgg ggcagataca cctgaaaggc gagaacgttt
    56341 tcgtacactt gcggagaatt cttggaatta cggatttttt gatgaggagg atcggctaac
    56401 aagtcaagtc atggcgaccc cttttccagt gaatttttac ggacaagagt acctaatggc
    56461 cggcattggc tatgttgctt cttatccaga agcaagggga caaggcggga tcaatcagat
    56521 catggaaaag atccttgaag attgccgtga tcgtaaagta agcttatctt atctggcgcc
    56581 attctcctat cccttctatc ggcgctatgg ctatgaacaa tcatttgata aaatcagtta
    56641 tcaactaaac agtcgtgatg tgccgtttat caaaaaaaaa tctggaaaaa taaagcgtat
    56701 ggattttgaa gatgcaaaag ctgcaatcag aagaatttat gaacagatgc cggaaaacca
    56761 acgagctggt ctgaagcgcg aagactggtg gtatacgtat aaattcaagc aaaaaggcaa
    56821 ttaccagttt gctttgcatt ttgatgataa tgaggaagca acgggatata tcgtttatca
    56881 gcttgcaacg tcttccttta ttattgcgga gtggggattt ttggatcatg atgctttttg
    56941 cagccttgtt cgcttcgtaa gttctcacaa tggtgctttt gaaacatttg aatattcatg
    57001 cggctacggc ggtaatgatc aaaattatct attagaaaat ccatttgctt ctgtaatgat
    57061 cacaccttat atgatggcga ggatcgtaga tatttctttg tttttggaaa attatccatt
    57121 taaaaagaga caagctgcat ttgccctaca aatagaagaa gatcattatg cgaaatggaa
    57181 tgaaggaatc gtggaagtaa caatcgaaaa tggaaaaaat catctctcaa aagtagaaca
    57241 aacaaaactc ccaatcgttc gaggaagtat ccaacatatc acgcagctat tattagggta
    57301 tcgtaaagtg gaagagttgg tattccaaga aagaatacag gtgacaaaag aatatgaaga
    57361 aacgttaagc tcaatcttgc cacagcaaca gccaattctt aatgactatt tttgataaaa
    57421 aacgggtgat gacaatattt ttgtcaccac ccttttgttt ttcatttggc atttgaagga
    57481 aaatgttaaa gtaaatcctc ttttttcgta aatttgaatt ttttgttttc ttttgtttta
    57541 gggttttctt ttttgtggtc ttggattgct tctttcgtaa tcttttcccg atttttttta
    57601 gatgaatcta agcgttctgc gtatactttt tcttttggct tatcattcat tggaaaggac
    57661 ctccctacat tatttcattc tacctctagt ttatcatctg ttaaggacta gtggaaacgt
    57721 tttgcctaac ttataagctg atttttttag cagataagct tgcaagtaaa aaacaaacaa
    57781 gctacaattg atttattgaa attgttcctg tagctcagta ggatagagca atcgcctcct
    57841 aagcgatagg tcgctggttc aattccagtc aggaacgtac aaataacccc tgtatccttt
    57901 gcggtacagg gggttatttt gtataagtag acattgtagt ttctaaactg ctatgtccta
    57961 aacgtttcga tacgctaagt atatttactc cttgataaag taaaatagat gcatgcgtat
    58021 gtcttagtcc atcaataaac agtcgaagag aaaataggga aaaattgatt actaaagaaa
    58081 aacttactag gtcgacggtg tagttgattt tcatacttca atagattcga attaaacgat
    58141 ttcgattggc ttataccagt cctatccaac gaaagatata tcgttcttaa ctctagcggt
    58201 ataacttgaa gctttacgta aagccaatgg gaatgaactg gagatgactt tttattattt
    58261 ttagtaatag ctgatgggtt aatacttaca actttccttt tcaaagtacc atataaaaaa
    58321 acggtggggt tgtttagcga aataaaggat ctaagccaat agcttagatc ctttattcta
    58381 tgctttatat tattttttgc atgtgtgcga aagtatactc aagcccaatc gccatttcgg
    58441 aaaatcggta ctgtagagcc atcttcacgg attccgtcga tatccatctt gtcagatcct
    58501 accatgaaat ctacgtgggt ttgacttcta ttcaatccag cttctgctaa ttcttcatcg
    58561 gacatttctg taccgccttt cacactgaag gcataagcag aacctagtgc taagtgattc
    58621 gatgcgtttt catcaaataa tgtattgaaa aagatgatgc cagattgaga aataggtgaa
    58681 gggtcaggta caagtgcgac ttctcctaaa cgacgcgcac cttcatctgt atcaagcagt
    58741 tttgctaaga catcttctcc ttgttcggca gaaaaatcaa caacttttcc atctttaaaa
    58801 gtaaatttca tacccgagat gatggtgcca gcataactca gtggctttgt acttgaaatg
    58861 taaccatcta ctcgatgact gtcaggtgct gtgaacactt cttccgtcgg catatttgcc
    58921 atgaattttt ctccacgagc attatagctt ccagcgcctt cccaaagatg gtttttaggc
    58981 aatccgataa tgatatctgt accgggagca gtatagtgaa gggcagaaaa ttgttctcga
    59041 ttcaattctt ctgctttttt tgctaatttt tcatcatgct tcttccatgc taagacaggg
    59101 tcttcttcat agacacgagt cgttttgaaa atctgatccc ataatgcctc aacttgtttt
    59161 tcttctggca gttctgggaa gacttttgct gcccattgtt taccagccgc agccacgaca
    59221 gtccaactga ctttatttgc ttgagttgcc ttgcgtaaat tcattagtgc tttgccattc
    59281 gctgattgat aagacgctac tcgatggcta tctacaccag caaatgcatc gggatcggca
    59341 gaaacaacgc tgatcctgct tgctcctttt tcaagccatt catccgcttg atcgatttta
    59401 gattgtggta cattctcaat acgatccgtt gcagcatgta aaagaaattc tctttggatc
    59461 tggtcgtcag tccattgaac gataacctca gcggctccta gtttataagc ttcttgtgtg
    59521 atcaatcgag caaggggtgc ctgttccacg ctgatctgta aaacaactgt gtgtcctttt
    59581 tctgtggcta cgccagtttc tgcaattaag cgtgcatatt tttttagaag ttcattaaaa
    59641 tcagataaca taataaaaat tcctcctcac cattttttct ggtaactatt agcatgatac
    59701 catttaatta tgattcaaac aatgaggctt taaataaaaa aaggaggtta tttttttaag
    59761 gttaagtttc atgatatatg ttactacttg ctatttcagc ttgtgaagaa aatcaaaaac
    59821 taattttgca tatcaaaaaa acatgttaca atgaaattaa tcaatggtgg aagcgttata
    59881 aaaaaattaa ggagattaaa aaatatggca agaaaaaaga cgattactag agaacagatt
    59941 ttggcagctg cttatgaagt agctgccacc gatggattta cacgatttac tgcgagaaac
    60001 atcgcgaaca aaatgaattg ttccacgcaa ccgatttatc ttgagtttaa aaatatggat
    60061 gacttgagac aagctctttt tgagcagatc tatgatcatt tacaatatga agtgttcact
    60121 gttgagcata cagggaatac gattatcgat cttgctttta attatatcca ttttgcaaga
    60181 agagagaaga agttgtatcg ttcactgtac ttagaagaat atggtggagg aaaagagatg
    60241 cagaatttct cttatactta ctttgtgaat acagtgaaac gcgatccagc ttatgtgaat
    60301 ctttcagaag agcagatcga atctttgtat aatgggacat ggatcatagc tactgggatc
    60361 gcagctttga tgtcttcaag catcatccat ccaactgatg aacagattga aaagatgatc
    60421 caagacagta tagatgctat cttgaaattc aaagaaccaa tcgaaatcga ttgaatatac
    60481 tcaaaagttc cacaaatcag taggtaacaa gagatgtgtc aaaataagga aaagttcgtt
    60541 tttcttacag cgtatgcatt tttaactccc gaagaactct tccatgaatg caatgaataa
    60601 aagagccata cagattgaat aaatctggat ggctctttta ttgcaagtat attaaatttt
    60661 tgcgactagt tctttagcga aagcttctaa attttcgata tcttcttctt cagcattcag
    60721 atccacttta acattttcgg ctccttttga tgcgcctgtt tttgcaaaag cagcatcgaa
    60781 atcatccacc gatttgcaga attcatcgta aaaagtatca ccagatccgc agacaccgta
    60841 aatttttccc aatagatcca attcttgcaa gtcttcataa aagtctacga tctcatcagg
    60901 cagatcgcca tcatcgtatg tataagtagc tactacgcaa atatctgcct cctcgaattc
    60961 atctgcatct acttgggtac attcgttgat ttcgacttcg atatccagat tttcaaatgc
    61021 ttctgcaaca atatcggcga tttcttcagt gtttcctgtc atgctagcat atacgatttt
    61081 tgctaaagcc atgatgtgcc tcctcgttta taattattta acaattatga attataccaa
    61141 aacatcattg aaaagaaaat aaaacctaaa gacacctggg gaatgtcatt tggctaaatg
    61201 tatgccgcta tgttaaaatg agagggattt gtaaaaagga gacgaaaata tgattacttt
    61261 aaaatcagca cgtgaaattg aaatgatggc agaatctgga gctttattag cagatgtaca
    61321 taaaaatctg cgtgatttta taaaaccagg aatcacaagt tgggatattg aggtatttgt
    61381 cagaaactat atcgagagtc atggcggaat tgctgcgcaa attggctttg aagggtatga
    61441 atatgcgact tgtatcagta taaatgatga aatttgtcat ggattccctc gtaaaaagcc
    61501 tttgaaaaac ggtgatttgg taaaagtcga tatgtgtatc gatttgaaag gcggcatttc
    61561 tgattcttgt tgggcctatg tagttggaga atctacacct gagatcgacc gattgatgga
    61621 agtaacgaag aaagctttgt acatcggtat tgagcaagca caagtaggga atcggattgg
    61681 tgacatcggt catgctatcc aaacttatgt agaaggcgaa aatctagcga tcgttcgtga
    61741 ttttattggt catggcgttg gtcctacgat tcatgaagaa ccagcagttc ctcattacgg
    61801 agaagctgga aaagggttgc ggttgaaaga aggcatggtt atcacgatcg aacctatggt
    61861 taataccgga acttggaaaa tgaaaatgga tcctaacggc tggactgcgt atacaagaga
    61921 cggcggactt tcttgccaat atgaacatac attggctatt actaaagatg gtccaagaat
    61981 tttgacatca caaggagaag aaggcactta ttgatctatc cagtcaatag gtacgggaga
    62041 ggtacatgaa agtttggaac aaaataaaac aaaatgaaaa tctgatgcgc tttattgaga
    62101 cgactcaaaa gcgcatcact gattcagaaa tgagtacgac ctctgtcgtc gttgcttatt
    62161 atttactctt gtctttattt ccattgatta ttgcttttgg taatatttta ccgtttctcc
    62221 acatcgatca agagactgta ctgacctatt tacgagaagt gattcctgag accgtttacc
    62281 agtttatcgg tcctgcaatc aaagaccttt tgacacaaag ttcaggaggc ttgttatccg
    62341 tttctgcatt agcggcttta tggtctgcaa gtcaaagtat caatgctttg caaattgcga
    62401 tgaataaagc gtatggcgta gaaaatagaa aaaatttttt gatcgtccgt ttcttctcac
    62461 taattgtcat tgtcttgttc atgattgcta ttagtggggt aactatcgtt cttgggttgg
    62521 gacagctgat tttggatgct attcagccaa tcgtacaaat acccgtgaac tttatcgatc
    62581 agttccaaac tttgaagtgg ccaatcacat tggtagcttt atttgtcatc atgttcttga
    62641 tttatttaat tgtccctaat gctcagttga agttgaagac gatcattccg ggcacgatct
    62701 tcgctactgt cggctggatg ctgttatcac aagtgtttag tatctatgct aggtattttg
    62761 caacgaggat cagcggttat cagatcatag gaagttttat tgtactcatg ctttggctta
    62821 attttgctgc tacgatcatc attttgggcg gaatcatcaa tgcagttgtt caagaatacg
    62881 tgacgggtca tcaaatcaaa aaacgtcgag atccaagccg aaaatggttc aataaattga
    62941 aaactaaatt ttctcataaa aaaaagtaga aacaagagtt cttattgtaa atttatatca
    63001 gtaatttata ataattatga ctcttaatta aagtaggtgc ccatatgctt gtttctttta
    63061 gtatggttat aagatttttt ctgacaattt tattatcact gatattaaca cctgtattca
    63121 aaatcatttc agtacaaaca ggtatggtcg acaaaccaaa tgaaagaaga ataaataaag
    63181 ttccaatgcc ttcagcgggc ggactgccta tctttatttc atttgttata tcgactttat
    63241 tcttattccg taaaattatc cctcaatcgt atattgtacc gatcttgatt gcttctttag
    63301 taatcatagt cacaggagtg ctcgatgata aatatgagct gacacctaaa caaaaaagta
    63361 tagggatctt gattgcttct cttattattt attttgtagc agatatacgt attgattcct
    63421 tcacgcttcc ttttattgga tatatccaac taggctggct tagttttccc gtaacgattt
    63481 tttggatctt cggtatcacg aatgcggtca atctgattga cggtttggat gggctggcgg
    63541 cagggatttc attgatcggt ttaattacga taggaatcat tggatatttt ttcctccatg
    63601 cttctacagt gtatattcca gttgttattt tctgtcttgt agcaagtatt atcggttttt
    63661 ttccttacaa tttttatccg gcaaagattt atttaggcga tacaggagct ctattcctag
    63721 ggtttatgat ggccgtcctt tcactacaag ggctgaagaa cgtgactttt gtttcatcga
    63781 tttctttgct gatcgtcatg ggagtgccgg taacagacac gttttttgct atcattagga
    63841 gaaaagcaaa ccgtgtttct ttttctacag cagataaaaa acatcttcat caccgactaa
    63901 tcagtttagg ctttactcat aaaggtgctg tcctgacgat ttatgcgatg gctttgatgt
    63961 tttctttcac agctatggtc atgaattata ctggaagaat cggaacgatc gtattgatta
    64021 ttgccatgtt gtttgctgca attttattat ttgaattgat tggtctgatc aatgagaaac
    64081 atcagtttat tttaaaaaca ttgcgatttt tagggaataa agagtaccgt acgcaagtaa
    64141 aacagaaata tttgaagaaa tggaaccgtc atatagatga ataacaaatt aaaccagccg
    64201 atccttatta gtattgtgac ggcaaacagt gataaaattt ttcggacact agataatttt
    64261 atttcaactg taaaagatga aacagcagta aaaatccgaa tttttgacaa caattcactt
    64321 cccgagtaca aaaaaagact aacagagtat aggaaatatc cctacatcga tatctatttt
    64381 catgatgaaa attccggatt tggttatgga cataatcaca atctgatagc tagcgattct
    64441 cgctatggca ttatttgtaa cccagatatt ttagtggatg aagagacagt tacagcattg
    64501 gtcttgttat tgaaagaaca tccggaatgt gcgatgttag cacctaagat tcttaatgaa
    64561 gatggaacaa ctcagcacct catccgtgag cagctggctg tttttgatta cgttttgcgc
    64621 tttgtgcctt tcaaatgggt caaaaaaaag tttgacaaac gactggctgt gtttgaatgc
    64681 cgaggattgc ctactgatgc taatagttat gtacggatga tctcaggtag ttttatggta
    64741 gctgatattg ataaatttaa agagataaag gggtttgatg agcgtttctt catgtacttt
    64801 gaagacaacg acttgtgttt gagttttgaa aaagcaggtt acaagttatt gtacacaccc
    64861 ttttatgcag ttatccattt ttacgggaag ggtgcgcatc gaaatgcaaa actgtttcgc
    64921 gtgtttttga cttctatgat gaaatttttc aataagtggg ggtggcgttt tttctgatga
    64981 aaccagaaat attagtagtc gttgtgcttt atcagcaact attcagtcaa tctccagctt
    65041 atggaccatt aagcaaagca cttagcgaaa agcaaattca gcttattatt tacgataata
    65101 gtcctcaaag gcagcaaaat gagctgtttg aaaaagaaaa tatcgtttat catcatgatc
    65161 cgcaaaatcc tggtttagcg gtggcttata attttgcgtt gtctcaagca gatgaaggaa
    65221 cacgttgtct cgtcactctg gatcaggata cgaggctagt cgacgattat tttgaactat
    65281 tgcgtaaagt aacctttaca gatgaatgtg ttgctgcagt tccgatgatt ttttctggga
    65341 gccgacaagt ttctccccta tattcagatc gttatatcaa tcgtacagct caagcagtag
    65401 aagttgaagg cccaacagct gaacgaatca tggcaatcaa ttcaggtgtt gcctggtctg
    65461 tcaaatttct aaaaaagatt ggcgggttta atcctgcttt ttctttagat tttttggatc
    65521 attggctgtt ttggaaagtg gatcagttgg aaaaaactgt ggagatcctt cctgtacgaa
    65581 tggaacatga tctttcggtt ttagactatg aaaaagtttc aagtaatcga taccgttcta
    65641 ttttacaagc agaaaatcga ttttatcatg aatatgatca aaggcattta tccagtcacc
    65701 gacgacaatt attgttaaga acagctaaac agttcttgac cgtcaaaaac cggcatatct
    65761 ggcggatgac tttgcgctca tatgttaaga attggaaggt gtgaattcgt gcgttcggtt
    65821 tgtatagcta cctttaatgg aggcagatat ttaagagagc agttagatag tattttatct
    65881 cagctttcag cagaagatga agtagtcatt tctgatgatg ggtcagtaga tgatacatgg
    65941 gatatattaa ctgaatacgc agaaaaagat aagcgaatcc agcttttttc ggggcctcgt
    66001 caaggattga tcgctaattt tggttatgcg atccagcaga cgaaaggaga tctaatcttt
    66061 ttggcagacc aagatgatgt ttggttggag acaaaagtga aaaagatcac tgactatttt
    66121 gagcagaatc cggatcagct agtagtagtt tctgatttaa tcattgtgga cagcgagctg
    66181 caagagatcc atgattcgta tttttcgttt cgaaattcca aagaaggatg gcttaataat
    66241 gtgataagaa gtcactatat tggtgctggt atggctttcc ggtcttcttt aaaacccgat
    66301 attttgccga tcccgtcaga agtgccgatg catgatatgt ggattggttt gctgggagga
    66361 aagaagactg ggttcctaag agaaccgtta actctgtacc gaagacatga tttaaacgcg
    66421 agtgagattg aaacacattc taaattacgg caaaaaattt cttggcgtgt tcatctgatt
    66481 acggcgttga tcaaacgaaa atggtttaaa cgttgataaa agatgtttaa atccttaata
    66541 tttccatgac aaatataaca attgatgaga taataacaaa gtgaaatttg aaatcaaaaa
    66601 ggagagcaga tatccatgaa gggaatcatt cttgctgggg gcagcggaac acgtctgtac
    66661 cctttaacaa aggcaacatc gaaacaattg atgccgattt atgacaaacc aatgatttat
    66721 tatccaatgt ctactctgat gttggcggga atcaaagaaa ttctgattat ttcaacgcca
    66781 caagatacac cacgtttcaa ggaattattt ggaaacggaa atgatttagg actgcagatc
    66841 gaatatgcag tacaagaaag tccagatgga ttggcacaag cgtttatcat tggagaagaa
    66901 tttattggag atgatagtgt ctgcttggta ttaggggaca atatttacta tggtggcggt
    66961 ctgtctaaaa tgttgcaacg agcagcttca aaagatacag gcgcgacagt atttggctat
    67021 catgtcaatg atccagaacg ctttggtgta gtagaatttg atgagaatat gcaagctttg
    67081 tcgattgaag aaaagcctgc tcagccaaaa tcaaattacg ctgtaactgg cctttatttt
    67141 tatgacaatg aggtaatttc tatcgctaaa ggtatcaaac cttcagaacg tggagaacta
    67201 gagattacgg atgtcaataa agcatatttg gataaaggaa aactatccgt agaacttatg
    67261 ggacgcggat ttgcttggct tgatacagga acacatgaat cattgcttga agcatctacc
    67321 tttatcgaaa caattgaaaa acgtcagaac ttaaaagtgg cttgtttgga agaaattgct
    67381 taccgtatgg gctacatcga taaggaacaa ttgctggccc ttgcacagcc attgaaaaaa
    67441 aatcaatacg gtcaatattt attgaatttg gccgctgaat aaggagtcct tacagatgaa
    67501 agtaacagat acaaaattaa cagatgtcaa aatcgtagaa atggacgtct ttggcgatca
    67561 tcgcggtttc ttcacagaaa gttattcaaa agcaaaattt gcagaacatg gattgaaata
    67621 tgactttgtt caagacaacc attcactatc agctgaagct ggtgtccttc gggggcttca
    67681 tttccaaaaa ggagaagctg cacagacaaa attgattcgg gtagtcactg gagcagtttt
    67741 agatgtagta gtggatatcc gcaaaggtag cccgacttat ggtcaatggg acggatttat
    67801 cttatctgaa cataatcatc gacagttgct tgtacctaaa ggatatgccc atggattcgt
    67861 tactttgaca ccaaatgtga acttccttta taagtgtgat aattactata atgcagaagc
    67921 agatgggggc atcgcatttg atgatccaga tttagcgatt gattggccaa ttgatccatc
    67981 aagagcgatt atgtcggaaa aagatcaccg tcatccgaca ttaaaagaat ttgaagcaga
    68041 aaatccattt gtttacggag aaatctaaga attaaggaga aattttctta tgaaaaacat
    68101 cattgttacc ggaggtgccg gattcatcgg ctctaatttc gttcattacg tggtaaacaa
    68161 tcatccagaa gtgcatgtca ctgttttgga taaactgact tatgcaggaa acaaagagaa
    68221 cttggccgga cttcctagcg atcgcgtaga attggtcgta ggagatgttg cagatgcaga
    68281 attagtggac cgtctagtaa aagaagcgga cgcagttgtt cattacgcgg cagaatccca
    68341 taacgataat tcattaaaag atcctttccc atttgtacaa acgaatatca ttggtacgta
    68401 tacactttta gaagcttgcc gcaagtatga tgtacgctat catcacgtat ctactgacga
    68461 ggtttacggc gatcttccat tacgtgaaga cctgccagga catggggaag gcgaaggaga
    68521 aaaattcact gcggaaacac catataatcc ttcaagtcca tattcttcta caaaagctgg
    68581 ttcagatctg ttagtaaaag catgggtgcg ttcttttggc ttacgtgcaa ccatttcaaa
    68641 ctgttcaaac aactatggtc cttatcagca tattgaaaaa tttattcctc gccaagtaac
    68701 gagtatctta agcggtattc gtccaaaatt atatggcgaa ggaaagaatg ttcgtgactg
    68761 gatccatacg aatgatcatt cttcagcagt gtggctgatt ttgacgaaag gcagaattgg
    68821 cgaaacctat ctgatcggtg cagacggtga agaagataat aagaccgtga tggaattgat
    68881 cttagaaatg atgggccaac caaaagacgc atacgaccat gtaaacgacc gcgcagggca
    68941 cgatttgcgt tatgcaatcg attcgacaaa attgcgagaa gagctaggtt ggcagcctga
    69001 gtttacgaac ttccgtgatg ggttggctga cacgatcaaa tggtacgaag aacatgaaga
    69061 ctggtggaaa aaagaaaaag aggccgttga agcagcatat gccaaaaatg gacaataata
    69121 cagaggaaat ttcactgtat taatggaaaa acacatgtga ggcaggcgct cgcctgtctc
    69181 ttttttaaaa taaaaatcaa ggagcggata accatatgtt tttattaact ggaggaaatg
    69241 gacaattagg tacagaattg cgccatttat tagatgaaaa aaatctgcca tatgtttcaa
    69301 cagatgcaaa agacttggat atcacagatg ctgagaaaac aatggcttat attacagaat
    69361 taaaacccga tgttattttc cattgcgcag cttacacagc agtagataaa gcagaagaag
    69421 aagcaaaaga attagatgag aaaatcaatg tcgatggcac aagaaatgta gcacttgcag
    69481 caaaagctgt gaatgcacaa ttcgtctata tcagtacaga ctatgttttc gatggaaaaa
    69541 acaaaaatgt agaatacgaa gtagacgacc aaacgaatcc actaaatgaa tatggtcgta
    69601 cgaaattatt aggcgaaaaa gcagtagaag agattttaga agactattat atcatacgaa
    69661 cttcttgggt tttcgggatc tatggacaca atttcgtctt cacgatgcaa cgcctagcgg
    69721 aaacccgcga caaactgact gtagtaaatg accagtttgg gcgaccaact tggacacgta
    69781 ctttggcaga atttatggta tatgtcatcg aagaaaaagc accatttggt atttatcatt
    69841 tgtcgaacga aaatagctgt tcttggtatg agttcgcaaa agaaatattg aaagatacag
    69901 atgtagaagt tgcacctgtt acgtcagaag aatttccgca aaaagcaaca cgtccgcagt
    69961 attcagtcat gagcctgaaa aagacagaag cgttaggttt tgtcatacca acttggcaag
    70021 aagcacttgc gcaaatgctg gaagctgcca aaaaaatgaa taagtaattt cctttttatc
    70081 aaatagaatt tggagcatga tatggaaaaa caaacaagaa gtgcacgacg aaaagaaaag
    70141 ccaaaagcga atcaggcaga ttcgaatttg gtgcttgacc tgtttctaaa actccgctat
    70201 cttattggcg ttttagtcat cgtggtcatc gttactttta atttaaacgg aagttcactt
    70261 ggtgtttggg ataaatatgt gtcgcaaaga gacgatggga aaaaaactga tgtgattttt
    70321 ggacagaatc gggaagtgcg gtcagacgaa tggctggtgc agacaccttt ttacctctca
    70381 caagctgaag aggacttttc gttggtgaac gaggattatt cattaaacgg acaaaacatg
    70441 atcattgcgt ataattctcc tgtaaaggat attacggtta tcggaaaacc attcaactgg
    70501 ggattctttt tcctcggaag agatcgagga ctttcctttt attgggcgat gaaattagtc
    70561 gtcatggtct tgcttggatt cgaagtactg atgatattaa cgaagaggaa aaaagctcta
    70621 tcgctaatcg gtgcatttgc gctgacattt tctccagcag tccagtggtg gttcatgcaa
    70681 catgtgggcg atttgatttt ctttactttg ggactgatga ttgcttttta tcattatttc
    70741 tacaatcatg ataaaaaatg gctacgaacg ttgatgatgc ttttggtggt tattttcggt
    70801 attgggttta ttttagtgat ttacccagcc catcaagtga tgctggcgta cttgctgatt
    70861 ttttattttg ttggcctgct tatttactat agcaagaaag tttcatggga tctgttggat
    70921 gtagtgttga ttggaggggc agttttattt attggcggag tcatggttca tttctggcta
    70981 acgtcaaaag acgcattgtt agcttctttg aataccttat atccaggaaa acgggtaagt
    71041 actggaggaa agtggacgat tggcgaattt ttttctttcc ttacaaattg gaaaattcct
    71101 tttgaagata tcaactattc gaataactct gaagtggccc tgttctatca cttttttcca
    71161 actgtattcc tagcaagtcc atttgtgtta tttggcaaaa aagatagcga acaaaagtta
    71221 ctgggacgta ttttgatgat tttttgtctg ttttctatct tttggattac cgttgggttg
    71281 ccaaaatggc tagcagaaat cactctgctg tcttatgttc ctccattaag agccatcctt
    71341 acttttagtt ttgcggcttg tttattatcg atctggttta tcgcttatat ttggagagaa
    71401 gatgttattc ctatttggta taaattcatt ttgttggcag taaatgcatg gtcttatttc
    71461 tatgcgatta cgcattcgaa aatggataat tatttttcgg attttgaaac catcgcagtt
    71521 gctgtgttgg gaacagtgat tttagctttt gcattatttg gctggagacg cttgttttac
    71581 ctcgcttttg tcgccttagt tatcgtatct ggctttttcg tcaatccagt cgtagaaggt
    71641 acaggagcga tttttgaaaa aacactggca aaagaaatcc aaaaaatcga tcaacaagat
    71701 cctggtaaag tttggttgtc tgaagatgaa ttatataact tcaccccaac attgggagtg
    71761 aaatccttca atacagtacg cttttatcca gatatagctg catggaagaa aatcgatccg
    71821 aaagaagaat acgagaagta ttataatcgg tatgctcata caagagcttt tatcgctaag
    71881 gaagatacaa agttcatttt agatcgtcca gatatgtttt cggtcacgtt gaattttgaa
    71941 gacgcagaaa aattagggat cgactatgtg atcagtaaac gaccattaac tgattacaac
    72001 caattatatg aagcgcagtt cacgcagcta tatggaccgg ataaagacgg ttatatgatt
    72061 tttaaagtag aatatccgga ttatccagat gtaccacaaa cacaaactca ggaaaatcag
    72121 ctagtcgtac agtaataggc tagctgagaa taatcctgaa atattagtag aaaggagagt
    72181 gaagataatt caagcaatcg tacaaaaatt tgtcaacaaa atgagtgtcg gtttcttgct
    72241 tttgttgctg acgctagtct tttttgtttg gacagcaggg gtaggttttc cagcaacttt
    72301 gtttatatat aacaatagtt cattacttat cgcttttagt gtggctattt tgctcatttt
    72361 aaatgtttat aaagcaaaag gcgtcgattt tctttatcta ggtgttgcgc ttgccctttt
    72421 cattttcttt tcttactttc gggaattaag aaatgagtct ttatttggag acttactgtt
    72481 tcctttggtt attttatttt gtttagtaat taaatggatc gagtggacaa aatgggatcg
    72541 cggtatttat ctgattgtct ttacggtctt cctcggaatc acgttatatc gtgtatttac
    72601 agagatccaa gtaccagaag gacaaagcat ctgggctcca ggcaattcgc tttctgatat
    72661 ttggatcaat acgaatacaa tcggaagttc tttgatgact cttgctttat tgatcagtgg
    72721 atttgccagt tcttttgaac gatggtatgt tcgaatcatc ggtgggatag cagtcgttgc
    72781 agccttttta ggtatttggg tctgccaatc tagaggtgca ttagtcgcgt tgattgtttt
    72841 tgcgatattg gatcttttac ctaaatcgtt gatgcgtgtc attcgtgcgc catttattgc
    72901 ctacacggtt acgattcttt tagctttgcc gatttcttat ctggcggctg tctctgaaaa
    72961 agtcaatttg tttaccgatc gagaagatat ttggcataaa ttctatcaga cgctaggaga
    73021 aaaatctgaa caagttttac taggaatgaa aacatttatc ttccagcgag gaaaccaatt
    73081 tttaggaaac cacaatagtt ataattctat tttgaatatc tatggattga taggatttgg
    73141 gattgctgct ttgctgctta tcctttttat tggtcgctta acgttgaaag cagatttatc
    73201 caacgggcag atgactttta tctgggcctt ttttgcggtg atgatgcagt catttatgga
    73261 ggatacattg acttcatacc catgggttcc gattatttat atcctgttgg ctatggccgt
    73321 tcatcgatat gatgaaccag aagttgcgga ggaattctat gttatggaag aggcatatga
    73381 cgatatgact tatgatgata catcttatga ggaacaacca gatagcgctc attcttccag
    73441 gatccaacgc tatcattgat aggcacaaaa tacgagagag gctgtgacaa ccgtcgcagc
    73501 cttttttggt ttattgcaat tgtctagcat cttctgtaaa atagaaacgt tagagaaaat
    73561 cttgtatcat agaaaattaa gaaagaaata agaacgcagc tcggtaccaa aaatatggta
    73621 aaatggcact tgactgtaat tcactgaaaa tcgcttggag gaacacaaat gtttaaagat
    73681 ttgtatttat ttatgaagga catttatcag aacagaaaat tattgctaca gttttcgatc
    73741 aatgacttca aatcaagata tgcaggatcg tttttaggaa tcttatgggc gtttatcaat
    73801 ccgctattta ctgtattgat ttattggctg gtatttggtg ttggactgaa agcacaaatg
    73861 atcgatgcaa aatacccatt tatcgtatat ttagttaccg ggatgattcc ctggttcttc
    73921 ttctcagatg catttgcatc aactacggtt gtttttcggg aatatactta tttagtaaag
    73981 aaagtcgtat tcaatattcg tatcttgccc acgacgaaaa tcctatccaa tctgtacacc
    74041 catttattct tcattctgat tggcatagct gtcgcgctta taaatggtat atatccttca
    74101 gcaatgtctt tacagctgat ttattattta ttttgcatgt gtgctttctt gacaggattg
    74161 acttggctga cggcatcgat ccaaccattt ttacctgata tcatgcaatt aattacgatc
    74221 gtgctgcagt tgatcatgtg gacattaccg atattatgga gccctaatca gtttgattca
    74281 ggtcttgtaa gcatattgaa attaaatccg ctgtactatg ttgttcaagg ttatcgtgag
    74341 tcatttttaa gtgaagcttg gttctgggag catggaacat atactttgta tttctgggtt
    74401 ttcacgttag taatgttcgt aatcggctct gcagtgttcc gtagattgaa accacatttt
    74461 tcagatgtgc tgtaaggaga atagctatga cagaatatgc aattagacta aaagatatca
    74521 caaaatctta taatatgtac gcaaaacctt cggatcgatt tcgtgaagcc ctcaatcctt
    74581 ttaagaaatc ctatcatgat gtattttatg cgttgaaaaa cgtgaatgta gaaataaaaa
    74641 aaggtgagat gatcggtttc gtgggagaaa acggatcggg aaaatctaca atgttgaaaa
    74701 tcattacggg agtactgaca ccaacatccg gcacgatgga aatcgaagga aaaatctcag
    74761 ctttgctaga actaggatca ggattcaatc ctgagtattc tggctatgaa aatatctatt
    74821 taaatggaat ggttctagga ttttcaagag aagaagtcga tgaaatggtc gatgatatca
    74881 ttgaatttgc tgacatcgga gaccatattt atcaacctgt aaaaacttat tcaagcggga
    74941 tgttcgttcg tctagctttt gctgtagcaa tcaatgtgaa tcccgatatt ttgattgtgg
    75001 atgaagcatt ggcagtaggc gatcttgaat ttcaactaaa atgtatggaa aaatttacag
    75061 aaatcaagaa ctcgggaaag acgattttat tcgtttctca tgatatcagt tcgattcgtc
    75121 gtttttgtga tcgttcctat tggttgaaaa acggagaagt cgtcgaatat ggcgatacga
    75181 tggaagtaac gacgaattat gaaaactttt taaagaaaaa atcagttaaa acagtggatc
    75241 ggaataaaaa ccaagtagaa cggttcgccg ctccagatat cgtcgacgtg acgaaagcgg
    75301 aattgttgga taaagacaaa aatccggtgg atatgctgga acaaggagaa aaactatacg
    75361 ttaaagtaac ttatgaagta aaagatgata caatcaaaaa tccggtttta ggaattgcgc
    75421 tacgaacagt cgacaatttt tatgtatgcg gacttaatac tctactagat ggtatcaaga
    75481 ttccttggaa aaaaggagag aatatctttt atctggagta tgaaaagtta ggtttgttag
    75541 ctggtgaata ttattttgac gttgcggtct ttgaagaaaa tgcaacggtt cctttggtat
    75601 ataagacaaa gtacatgaac ttgtttgtca gcggttctta tatcggagaa ggcatcgtgg
    75661 tcttagacca taaatgggaa gaagggactc atagcaatga aatatgattt tgaaatggac
    75721 ctagatgaac aaagcagtgt gggaaaaatt gccgcacaga tcaaaccagg aagtaaagtt
    75781 ttagagtttg gtcccggaaa cggccgatta acgaaacatt taatcggagc aaaacaatgt
    75841 gaagtgagca ttgttgagtt agataaggaa ttgttcgatt ttgtcagcga atttgctcaa
    75901 gatggatttt atggtgatat cgaaagtttt gagtgggcaa actattatgc tggacagaca
    75961 tttgattacg tcttgtttgc ggatgtttta gaacatttgg tagatcctgg aaaaacattg
    76021 aagaaagtca gagaattttt gaatgaagaa ggagagatcc tgattacatt ccctaacttg
    76081 gcgcataatt ctgtcatgat cgatttgttc aataaccaat tgccgtgggc ttcttatggt
    76141 ctcctagatg aaacccataa ttctttttat actcatgatg gcttccaaaa agtttttgaa
    76201 aaagcgggat tattcattaa tattgaagat tatttgtact tagctgtagg tgatacagaa
    76261 ttaaaatcaa cttatgaaga attgccggaa gctgtacgct atgatttcaa aatgcgacca
    76321 tttggtgaag tctaccaata ctttttctca ttgatgaaac atccagtagc acaaagttcc
    76381 attgcagagc cacaaaattc taattatgta aaagtgttag aagtaaccca gcaaacgaaa
    76441 caagatgaaa caatccaaca aataccattc aataatttca caggggaaaa tgagacacta
    76501 agtttccccg tatcagaaac aacagaaaga atggtgtttc gcttcgcgca acaacctagc
    76561 tttatcgaat tcagcgcaga agctgccgga gaaaaactaa catttatcga ttcgaatgct
    76621 gtagtcaaaa cagtaaatga ttgttatttg tttgatggga aagaattacc agagttcgtt
    76681 ctaacggata tttctgggaa agaagtgact atccattgtc actatcgctt tatcggggaa
    76741 ttgacaccaa caatgaaaga attgttggaa acgattcgtc ccatggctga agtcacaaag
    76801 cagctttctg agaaaaatga cgaattagaa agagaaaatc atcatcttct tgaggaaaat
    76861 acaagactag accatacgtt aaaaacaacc acaaatcgct attgcacatt gttggaaagt
    76921 gatgaatgga caataaaaca tagactagga cgacgtaaga aagaaacttc gaaaaaaatc
    76981 caagaaaaag aactttctat atgtattgac gagaaaatct gggatgcaga aacaaaaata
    77041 ttaaaaatca tcggatgggg aatcgctaat tcggatcgcc agcccttgtc ctataagtta
    77101 tcagctgacc aatcgccatt tttcgaagca attcctgttt cacgagaaga agtcaatcaa
    77161 gcagaacagc tcgcaaaagg aacagaagcg ggatttgagt tacgaatcct ttgcgagcaa
    77221 gaacgttcat tcttagtgga agcagtagct caaaatggcc aatcatggat cattgaaatg
    77281 taaataatga ttaacttata aagataagga gtaaacgaat cgtgcaaaaa aaagaaattg
    77341 ccgtatttat cgatcaaatc actcgtgaac gggccactgg cgatttgctt attgtgggct
    77401 gggcaattga tgaagtgacc aaagaaatcc ctacgataaa ggtagaaaaa gaaaatgtga
    77461 ttgctgaagc aacacatgtc gtaagattgg atatcaatca tttatataat cttgatgtta
    77521 agacccaaag cggtttcaag gtacgtttat cagggaaaat gagaggaaaa gccatcttag
    77581 acttccaaac agcgagacat caaaatggca tagctgtgaa attaaacggt aaataccctt
    77641 atgatgacgg cattgaatct tcatgggaaa gaaaaaaaag attactgaaa aaagggataa
    77701 attatgcaag gactcatggc gtaaaaaaag cgatccgtcg ggtcaaattg gaattgaagc
    77761 caggatcgat cgactatgca gagtggatca gtcgtcatga gaaaccagat ttgaaagagc
    77821 aaaggaaaga agcacaagga tttgcgtacc gtccattgat ttccatactt atgcctgtct
    77881 ataacgttga aatcaaatgg ctcgaaaaat gcattgattc ggtactggat caaacgtatg
    77941 atcattggga actatgtatc agtgatgatg cttctactga tccagctatc cgaaagtgtc
    78001 tggaatccta tcaagcaaaa gatgatagaa tcaaagtcgt tttccgaaaa gaaaatggac
    78061 acatcagtct ggcaactaat tctgcattgg aaatagcaga aggagaattt atagcactct
    78121 tagataatga tgatgaatta ccgccatttg cattatatga agtggcaaaa gtgctaaacg
    78181 tacatccaga actggatgtg atctacagtg acgaagataa aatcgatgca gatggcaatc
    78241 gctttgatcc gcatttcaaa gcagactggt cgcctgatac attgatggga aataattata
    78301 tctctcactt aggcgtatat cgaacaagca ttgttaaaga acttggcggt ttccgtaaag
    78361 gctatgaagg ttctcaagat tatgatttgg tccttcgtgt gacagaacaa atccctgcag
    78421 atcatattta ccacattgac cgagtcttgt atcattggcg gacgatccca ggatcaactg
    78481 caagtaatgg cgaagccaaa agttatatct atgattccgg agtgaaagca ttgacagatg
    78541 ccctgtctag aagaaacatc aaaggaagcg tacgtcctgg cagaatttct ggattttatg
    78601 agattgctta tgacgtcttg caagaggatt tggtcagtgt gatcattcca acgaagaatg
    78661 gctatgaaga tctaaaaact tgtgtggatt cgatcatcga aaaaacgtct tattcaaatt
    78721 atgaaatcat tattgcggat aacggaagta cagatccaaa aatgcaagaa ttatttgcag
    78781 aatacaagca tcagctgaaa gaccgcttta tcgtagaatt gattgacatt ccattcaatt
    78841 attctcgaat taacaattta gcagcagaaa aagcaaacgg caaatacttt ttattcttga
    78901 ataacgatac agaagtgatt gagccagatt ggatgacggt gatggtttct tatgcacagt
    78961 ttgatcgaat cggctgtgta ggagcaaaac tgttttatcc agatgataca acgcagcatg
    79021 caggagtatt attaggaatc ggtggtgttg cagggcatgc attgaataac tatgatcgaa
    79081 cacattgtgg ttatttcggc cgattggtca ttgacgtcaa ttacttagca gtcactgcag
    79141 cttgtatgat ggtcaaggca gctgacttca atgctgttaa cgggtttgat gaaacattag
    79201 aagtagcatt caacgatgtt gacttgtgct tgaaagttta tgaactgggt cgttacaatg
    79261 tctatgcaca tcaagcagaa ttgtaccatt tcgaatctaa atcaagaggt tatgaagata
    79321 ctccagaaaa acaaagacgt tttgctggtg aaatcaaaaa aatgcaagat aaatggccag
    79381 catatattgc gcatgatcca ttttacaatg ataatttaac aaaagacgga atcggcgact
    79441 tttcgttgag accagattaa actataataa aaagctgtta tgttgtgatt cgctcgcaca
    79501 taacggcttt ttatagtttt ttgtcaaaaa aaggcagaag gtgccgactc aaaaaaataa
    79561 gtcgaggttg ggacagaagt gtttaactcc gagaaataag aagaaattca cgaaaattgc
    79621 ttttcaaatt tttgtgaatt tcggcttatt taagaagaag ttgcttctgc tcccaccgtt
    79681 tattcgtttt taaagaatga gacaaaagtg tttcttactt ttgtcccacg ctcttattca
    79741 tgctcagagc gccaatagat acaaaatcat agccaaagtt tcacgaaccc tagctgtcaa
    79801 agaatcgata ttttatttta atataggcta ttttaaaata aatggtttaa ggtattcctc
    79861 tacaaaagcc actctatcaa ttatcatggg tttaaaataa gaagtgatgg agataaccag
    79921 attgttctta gcgtcaatat aaagaacatt tccactatta ccaattgctg aatatatttc
    79981 gttctcagaa tctaataccc accataaata gccatatctc atattttgta agtcttctaa
    80041 agcactggtt ctaggtttga acatctcgtt tatccacatg ggtggtacga tttgttttcc
    80101 tttatacatc cctttattta ggcataatac accaattttt gccagatcac ttgccgataa
    80161 acacaaacca tatcctgccg tcccgatatt tagtggatca caaaaccaga cattggtttt
    80221 aggagactta tccattgtaa actgcttatg ttcttcagct gtttcagcat aataattgac
    80281 atggtttggt atccctatag gatcaaatag atacttattg gcaaaatcaa cagttgttag
    80341 cttacttgtc tgatacaaca agccagttaa tatgtgtaaa cagacagttt gatagttgaa
    80401 ttcgccggtt ataccttttc ttcctcccaa taaatctagt gatgtttttg tccaatcttc
    80461 acttgaacag acttttgtcc atggatcgcc tttacatttg tagggtgctt tcattgttag
    80521 caagtgcttg attgtaatag cgtaaatagt tttctccccg cgtttcactt tatactctgg
    80581 aaaataatct aatactttat catcgatact tttgattaat ccttgctcta aagcaatgcc
    80641 aatcagcaaa gatacaatac ttttcgttgc tgacatgata tgacaagtat cgtctttttt
    80701 ataaccattc cattcatctg tataaacttc taccccatcc ttatatgcga caatttgaca
    80761 tatatttggt tgtcgaaaac caataaaatc atgcaactct tgtttattca taagatatta
    80821 cctcactttc ttctataagc ataatgtcat taaaaatcga tttcaatttt ttcgattctt
    80881 cacgtaatac aggtattctt atggactaga cagcgctttg ggaagatgga ttctctgaaa
    80941 aatataaagg ttcactttat gagtggataa aaaaagcagc aaatacttcg acttctttaa
    81001 tttaccaaca ttagtgaaaa tcgattcatc tggagatttg taatttagag tccttgtctt
    81061 gattagaggt gtataaagct ttttattaga catttttaaa ttccattaca atttgcttat
    81121 aaactaggtt tttaaaggtg actatggtaa gataatagag atattatttt gtgattagga
    81181 gtgtatatat gaataaaaat ggagaatgga gtgctgcacg acggatattc attatattaa
    81241 tggatgttat tgtatacaat gcagcaatat atagtagttt cttactgaag tttcgtggag
    81301 agatcccatt acgaaattta gaaactttcc aacactcagc aattattatt tcagtgattt
    81361 ttattgcgtt gaacattttg ttgggtgcat atgtttttta taatcgaatg atcagtgaca
    81421 tcgtgtttgt cacagtcatc ggtcaggtat tgatgacact agggatcatg gtagtgactt
    81481 ttgcgggccg ctggtttgct tttccacgtc ttgttatctt actttcattt gttctcggta
    81541 cgcttttctt aagtatctat cgtattctcg tatataaact ttatttacgc gtaagtcgag
    81601 ataaaaaggt cgtcattatt gggttagaaa aggatgtcgc accagcaatc caaaattttg
    81661 gttcaaagaa aaacaacaaa cataaagtcg aagcagtcat tcttgataac tattatgcaa
    81721 acatcaaaaa tatgattaaa gacatcgata ttgtctatct tgcttctcat atcgaagaaa
    81781 ctgaaaaaat caaaatctat caattagtta ctaaaaatga aaaaaaattg tttttgaata
    81841 caacatttga aaacttgaca ttgatcaatc caaatattat gaatttcgaa gatgaaagta
    81901 ttattgaagc atctggattt agaatccaac ctgaatatga actattcaaa cgtttgtttg
    81961 atatcatcgt ttcacttgtt ttattagtta ttgcttcacc atttatgtta ttcacagcaa
    82021 ttttagtcaa agtgacatca ccgggaccaa tcatctataa acaagtgcgg atcacaaaaa
    82081 atcaaaagga attttcaatc tataaattcc gctcgatgac cgccacagcc gaagcaaaat
    82141 ctggaccagt tttagctaag agcaatgatg cacgagtaac gcctgtaggt aaatttattc
    82201 gagctgtccg ctttgatgaa ttaccacaaa tcttcaatgt gctgaaaggc gatatgtcga
    82261 tcgtaggacc acgtcctgaa cgttctttct tcgtcgatca gttcaatgca gaaaaccctt
    82321 attactactt acggcataat gttcgtgctg gtatcactgg gtatgcacaa gtctatggaa
    82381 aatatgtctc tgattacaac agtaaattac gttttgattt actttatatc aaaaaatatt
    82441 cattgatgct tgatttaaag atcttgctgc aaaccatcaa gatcctattt gataaaatgt
    82501 cctctcaagg acttgaagaa gacgaaaatg tgatctcatt ggcagaaatt gcttttccga
    82561 atgaaaaaat ctttcgctag ttgggaggcg taaaaatgaa caatccgaaa ttcagcataa
    82621 tcgtaccagt atacaatgca gaagcaacta ttggtcgaac gattgatatt ctaaaagaat
    82681 tggattatga agagtacgaa gtcgttttag taaatgatgg atcaacagat caaacacagg
    82741 aaatcattca agaactgatt gaaaatgatg aacgttttga attgattact acgaccaatc
    82801 aagggcccgg attagcaaga aacgaaggaa taagacagtc aaaaggagaa tatctcttat
    82861 tcttcgatgc agatgacacg ccagtaaaaa cgatcttgtc agattatgat catttgctag
    82921 aaaagagtcc aaaagcggat cttgttgtta gttcgtttgt ttttcgtaca atggacgagg
    82981 gaagaatcgt ttccgaaaaa gaaaacttag ttacagaatg tcactatcta actcacgaag
    83041 agtttataaa agacatgtat cagttaatga atgaacagtt gatgtatgtt gtatggaaca
    83101 aatgctatcg tagcgatatt ctgaaaaata accaaattct atttaaaact tataatagtt
    83161 gtgaggatcg aatatttaac ttacattatt atgaatactg tcatcaagtg atcatgaacc
    83221 caaaagtaga atatatttat gaatttgaag gtgggaaggg gatcactaat caataccgac
    83281 ctaacaagtt cgaaacattt aaagaattct attcattagc aaatactgta acagacgata
    83341 ttaataaaga cggtatggcg gcgcttcttt taaaagggac aacttcagtt attttttcta
    83401 tttatagtac ctctgcaaga acttcaaaag agaagaaaga cgaagcaaag cagatattga
    83461 gtgatccagt gatccgagaa gccaagcaaa ttgctaagac ggattcttca gcaaaaaaaa
    83521 taacaaagtt gctttacaat atgccagctc cactattttt atctgctgta aaagttggaa
    83581 gcttcgttga agtgaaaatg ccagggttaa tggctgcttt gaagcgagta tattagttaa
    83641 tggaggaaaa tataatgaaa agaattttgc attttcaggg acgaatgggt ttaggtggag
    83701 cagagtcttt catgatgaat ttatatcgta aaattgatcg tacaaactat caatttgatt
    83761 ttttgattta tgaagattat gcagatgttc aagactatca ttcagagatt gaaagattag
    83821 gaggccgtat ttttgttgta cctaatccta agaaaaatat ttttaaatat ttaatagaag
    83881 tgaataagtt actaaaaaaa gaatcttttt ctatagttca caatcaggtt tattttggcg
    83941 ggggaattaa tttatggtta gccaaaaaga atggaatcag acagagaatt gctcatagcc
    84001 atgcaactga agatggaaaa tctcaaaata ttgtaatgaa tgtattgcgt aaatttttaa
    84061 ccaaattgtt gttacaaaat gcaacagact accttgccgt tagtcaagaa gctggtgaaa
    84121 gtctgtttca aaaccatccc tttgagatag ttcataatgg tattgattta gagctttata
    84181 gtaaaaattc agaagctaag gttaataaga gaaaagaatt ggatatctca atgagtactt
    84241 ttgttgtggg aaacattgga cgattagaga aacaaaaaaa tcaatcttat ttgttagaga
    84301 tttttttaga aatactaaaa atgaattctg attcttactt gattttagta ggtgaagggt
    84361 cacttagaaa taaactgaaa gccaaagcag tagaattgga tatagagaaa agagtaaaat
    84421 ttttaggaga aagaaacgat attcccgagt tattatctgt gatggatgtt tttgtaatgc
    84481 cttctcttta tgaaggtctg ccaatggttg gattagaagc acaagcagca aaattaaaat
    84541 tagtcttagc agatactatt tccaaagata caaagttaac aaataatgtg gattttgtat
    84601 cattagatga aacaccacaa gaatgggcaa aaaaagtaat aaaagagccg tatgctaata
    84661 aaataacaca agaactaaag agttatgatg tttcgtacac tactgaacaa atggagagta
    84721 tatatagtag agatcattaa aaatgttcaa gatattttag gagtgattat ttttgaaaat
    84781 atgttatttg atattggcat acaatgatat tggcaactta aagagattgg tcaatcgatt
    84841 gaacgaaaat gcagttttta ttattcatat agataaaaaa actgatatag aaccatttat
    84901 tgaagcattt aaatcatatg ataatgttga atttttatgt aataaaaacc gtgttaaaat
    84961 ttattggggt ggtttttcca ttgtacaagc agaaatcaat ttagtaaaga gagcattaca
    85021 aaatgagaaa tatttaaaat atgtattatt atctggtgct gattatccaa tcaaagataa
    85081 tgagtatatt tataattatt ttaaaaagaa tagttcagtg gagtttattc gtggaataga
    85141 tctagatcaa attaaacata aagaatttta ttataaacat atagatgtat atcaaaaaca
    85201 tgattatcct agaattaata ggaataacac aactgcattt aaaattttta gagctataat
    85261 taatcgttgt ctgaggatga ttaaacttcc cccaaaaatt agacaccata aatttgattt
    85321 gtatcatggt tctcaatggt gggctttgtc gaaagaatgc ctaacagaat taatacaaat
    85381 gtatgaacaa aatcaacaag actacctgaa ttttaaaata ggtatgtttg ccccagatga
    85441 aaaatttttt catacacttt tttttaattc ctcgttcaaa aataaaaatg ttattggagg
    85501 accagattta ccacttgaat tgaaaaatat tgaagaaact actttacaaa cttcgaaatt
    85561 agcaaatatt catattattg atccttccat gaacaaatgg tttgatgaaa gggatataga
    85621 tcaaataaaa gaaagtgaaa aattatttgt aagaaaagtt aaatcggatt actctgataa
    85681 actattaaat agaattgata aagaaatttt gaaagttgga aatatagatg aatagaatag
    85741 tacctgtttt agagaagagt ttattaagtc gagatgcgtt tcgagtaata aatttaatca
    85801 tttgtctgct ttattttatg cctagtactt ttaatgtaac taatatcccg atgaaaatag
    85861 cttttgcttg gggtggaatt atagttttgt atgaggtttt tataaagaaa aattttttta
    85921 aaatgcgttt ttcttggcta ctgattggag caatgattag ttttatctta actataatca
    85981 ttaacagaga aaatttttta attccatcta tttataattt tggatatttg ctgattactt
    86041 tgatagtaat ttttcctgca gacttttcac aaagtgaaga ggaaaaaaag aaaaaaatgg
    86101 tgtattttaa taatgttttc attatcataa tttttttagc tgcgctaata tctatctatc
    86161 aatttatttt tttgatttct tatcatgtcc caacaggaac gccaggatta cttgcaagac
    86221 aaggttttat tgaacatcgg ttatttggtg tctatacaag tccaaatgtt ggagctatgt
    86281 ttggatatac ttctattatt ttaagtttaa ttagtgtatt aatttcaaaa ataacaaaaa
    86341 cgattcgtgt tttttatatg atgaatttta tcgtacaaat attttatatt acactttctt
    86401 cgtctagagg tgcccaggta gtaatttgtt cttttttagt attatttctt ttgttgtttg
    86461 ccaattcaaa atttagaaag agcattagag aagtactacc tgtaaaaatc aaatggatgg
    86521 taagcatatt attagttcta ttggtatatt tttcttttgg tactaattat gctaaggatt
    86581 ttttagcaac aattcctgta tcgattgata gaaaagtaga agttatagag tcaaatgaat
    86641 cagataaaac tccacttcca gaaaaagaag taatattaca gcattctagt gataactccg
    86701 aaatttcagc tggtcgtttt acgatttgga aagctgcgct taaagttata agaccaactt
    86761 tgttatttgg ttatggagag accgatttct atgggaatag cgatgcaaaa tttttgaaaa
    86821 aagtagatct tgatactaca gatgtgaatg aactaaaaag agcacatgga aacatgcata
    86881 atggatttct tcaagtactt gtatcaagtg gtataattgc ttttatatgt atctatgctt
    86941 tctatgtgtt aaatatcttt agtttactca aaacattttt tagaagtaaa gtgaattatg
    87001 cgtttcttgg tattgtttta atttttatac tatctatttt tatagatgaa atggtggagg
    87061 ctcatgtact tttcaataag cgcgatgtca tttcaattgt tttctggtat tatttgggaa
    87121 gcatttctta cttgtattta aaaaaggaga attaatatga agacagcttt cattgcttgg
    87181 actcctttac atttgattaa tatattaaat gtttatcaaa atttttaccg agaatcaaaa
    87241 ggagatttat ttatttattc tgaatttgaa aacgctagaa gatatagtga gcgtataaaa
    87301 gaattagggc tatttaatga tgttgttttt gtgaatcacc ggaaaatcgg ttcttccttt
    87361 aaacgtaaaa ttaacttgtt aactaaccat aatcaatttt tctcggatga tttatccgtt
    87421 tatgatgaaa tttttataca gggggaaaat tatttcacaa aaatactttt ctccaatgtt
    87481 tacaaaaaaa atccagatgt aaaactaaat tatatagaag atggtttggg tacttatttg
    87541 aagaagagta tattttcaac aagtaaaaac gttcaaagag tttttaaaat attaaataaa
    87601 aatagcatct tcaaaaaaaa aattgacgat tattttgtct attgtccgga attgttactt
    87661 tatgatgtaa aaaatataaa aaaaattccc aaaatagatg aaaacagtaa attgatgaaa
    87721 aacatacaat caatctttgg gatagaagaa gtttttgtcg gagataaatg tatctttttt
    87781 gatcaacctt tattatctga tggttatggc atagatgaaa cagatatttt cgctaaagta
    87841 aatagtatat tttctggaaa aaaagaaact ttaattaagc ttcatcctag agcaaggaca
    87901 aatcgttatg gtgaaaaaaa catattgaat tccagtttac cgtttgaagt cttgctactt
    87961 gaaaatgact tctcttcaac acttttgatt agtcctatat ctacaatttc tttttcacca
    88021 tctatgatgt ttgaaaagac gatcaactct attatgttgg ctaaattaat tttaaatgaa
    88081 tatacagcat tatctgaaga taaaaaagaa acattgacaa atattgttct tttctgtgaa
    88141 cattataacc gtctatcaga agaaaaaatt ttactcccag aaaactgggc agaattaaga
    88201 aagttggtag agtatgggat tatttaacac actttccaag tcaaaattag ttaagtccag
    88261 tttctggtat actataggga atttttttat taagggaatt tcattcataa ctattcctat
    88321 ttttgtaaat attactactc aagcagaata tggtttgatt aataatttca cttcatttgt
    88381 atcaattttt tcgattcttg ttgggctaag cctaaattcg gcaattaata atgcaagttt
    88441 tgactataaa gatgacataa atgaatttat gtcatcggta ctttctctag ctacactttc
    88501 gttaattgtg ttcttattaa tagggaacat atattttttg ttttcaaatg ctttttttga
    88561 tatgaaccaa cttgtattta atttgatgtt attacaaagt tttgctagtt ttgtaattag
    88621 ttatatccag tcttatttta cgattaactt taaccatttt aaatatttga ttttatcttt
    88681 tatatctact tttttgaata ttgcaatctc tgtagctttg atgcagacat tatattttga
    88741 tgataaatat gttggcagag tagtcggcgg aacactgtca tttgtgttga taggtttagt
    88801 aatttattta agaataatga taaagggtgc tcattttttt gataaaaaac agtggagata
    88861 cgctttaaaa atctctttgc cgctaatccc acattcctta gcaaatatag ttttagcaca
    88921 attcgataga attatgatta attcttattt aggtcctgct gcggggggaa tatattccta
    88981 cattagtaac ataggtatta ttctaagcgt aatatggatg tctactaata acgcctgggt
    89041 tccatggttc tatggtgaga tgagtaaaaa taatgaaaaa aaagtgaaga aagttagtaa
    89101 ctattattta gtagtcttta ctttgattac gatgattgta atggtagttt cgattgattt
    89161 agcaaaattt atggctccga aagactatta ttctgggtta tctttagtgg ttccgataac
    89221 cttaggctat ttctttcagt ttttatatag tttaccagtt aatgctgaat tttatgagaa
    89281 aaaaacatcg tttattgcga ttggtacagt agcaagtgct atagttaata tactattaaa
    89341 catgattttc atacctaggt taggaataat tgctgctgga tacacaaccg tatttacgta
    89401 ttttttactt tttatttttc attataatat cgctaaaaaa attacgaata aacaattatt
    89461 tgacacaaaa aaaatatgta tcataataat ttttgtatca attttttctt tgatacttca
    89521 tattttaatc aatcaaatgt tgattcgtta cgttttgaca tcattattga taattagttt
    89581 agttatatat tttttaaaga ttaagaatcg aattatagat gactataatt cataggagga
    89641 gatataagtg tttaaaggaa aaacaatatt agtaactggc ggaactggtt ccattggaag
    89701 tgaaattgtt cgtcagcttt taaaatatga accaaaagct atcagaattt atagtagagg
    89761 tgaagaaaaa caattttata tgcaacaaga gctctcaaat cacactaatt tgagattttt
    89821 aattggtgat atcagagact ctcaaaggat tgattatgca tgtcgtgatg tggatattat
    89881 ttttcatgct gccgcaatga aacacgttcc agcaagtgaa tataatccta tggaagctgt
    89941 aaaaacaaat gtgatgggaa ctcaaaatgt aatagatgca gcgatcaaaa ataatgtaaa
    90001 acattttgta ggtatcagta cagataaagt agtgagtcct actaacacca tgggtgcgac
    90061 aaagttattg agtgaaaaat taataatagc tgccaatcag tataaaggaa aaacagaaac
    90121 aatattttct tgtgtaaggt ttggaaatgt catggggtca agtggatctg ttattcctct
    90181 ttttagtaaa caaataaaaa acaataaaga tattactttg acagatgttg atatgacacg
    90241 atttatgatg tcaataccgg atgctgcaag attagttctg tctgctgcta tgaattcaaa
    90301 atttggagaa acatatgtct taaaaatgcc ggcaatcaat attttaactt tagctcaatc
    90361 attaataaaa atatataatg atacacatga tgaaaaatat agaggtgaaa taaaagttgt
    90421 aggtatcagg ccaggagaaa aattatatga agagctaatg actacagacg aaattgaacg
    90481 ctcatatgaa aatgataaaa tgtatatcat tccaccacac tatcatgcta ctgacgaact
    90541 gtacaaagat tatgaaaaaa ttaaaatcac tgcatactct tcagatgcaa cagaattaat
    90601 gagtggacag gaatttatta gaatcgcaaa agagaataat gtcgtgactt ggtaaggagt
    90661 tttatgatga aatcagaagt atatgtaatt gcagaaattg gtgtaaacca taatggaagt
    90721 ctagatttag ctttagaaag tattgataaa gctaaagaat gtggagctaa cgcggttaaa
    90781 tttcaaacat ttaaaacgaa taagctagtt tccaaaaagg ctccaatggc aaaatatcag
    90841 aaggataatc ttaaagagga cagttctcaa tttgaaatgc taaaaaaatt agaattatct
    90901 caatctgatt ttattagaat aaaagattat tgtatacaaa ataaaattga ttttttgtct
    90961 actccttttg atgaagaaag tgcagacttt ctaaaacaaa ttggtgtaca aggatttaaa
    91021 attggctcag gagatttaac aaatttacct tttcttagaa aaatagatat gtatgggttg
    91081 ccagttttat tatctacagg tatggctgat attcatgagg tcagtgaggc tgtagattgt
    91141 ttcatagata gtccagttac tattttacat tgtacttcaa attatccggc tagttctgaa
    91201 gatattaatt tattggcgat taacacatta aaagaaatgt tcgggatacc cgttgggtac
    91261 tccgatcatt cattgggata tgatgttggt atttgtgctg ttgcgatggg tgctaaggta
    91321 attgagaaac attttacctt ggataaggaa ctacctggtc cagatcataa agcttcttta
    91381 gatcctcagg aatttgcaga ttttgtaaag catattagaa acactgaaat atttttaggt
    91441 gatggtgtta agcgagcaat gccttctgaa cttagtacaa aagaagtatc aagaaaaagc
    91501 ctagtggtca ctgagaattt aaatgtggga gatattctta cagagaaatc tcttacaata
    91561 aaacgtcctg gaaatggaat agctcctaag tttttatatc aatatattgg caaaaaagtt
    91621 aaaagaccaa taagtgcaga tagtgttctg cttgaggaag atgttgaatg aaaaaaatac
    91681 ttatagtaag tggaacacga gctgattatg gaatatataa acctgttgct gagaatttac
    91741 tacaagataa ttgggatgta tcatttgttg tttctggaat gcatttatcc cataaatatg
    91801 gatttactaa agaattgatc ttagctgacg gatttagaat tagtggagaa gttcaaagtt
    91861 tgtttttaga gaacaataag ggaaacatgg ctcggtctgt ggcattagag ataaatggtt
    91921 tcacaaatat atttgaacaa actagccctg attttgtatt attactaggg gatagaggag
    91981 aaatgttggc agctgcgata gcttgtttgt atcttggaat caaaacagca catattcacg
    92041 gaggcgaagt gtctggtact gtagatgaat caataagaca tgccataacg aaaatagcat
    92101 ctgttcattt tgctgctacg gaaaaaagca aagagcgttt aataaaaatg ggggaagatt
    92161 cttggagagt ttttcccgtc ggagctccta gaatagatac tattttaaat aatagattac
    92221 ctagttttag agatataaaa gaaaaatatc atctaaaatt tgaaaagaaa aaatacgttt
    92281 tgcaagtatt tcatccagtt gtaaccgaac tagaaaatat agaaaaacaa attttagctt
    92341 tgatcaattc aatggaacaa caatctcaac caattatttg cattttacct aatagtgatg
    92401 ctggtagcga aattatcaga aaaatgtacc agaaatattt aaaaacggat attatttata
    92461 ttacgaattt agagccggag gaatacctga ctatattaaa agagtgcaaa tttatgatcg
    92521 gaaattcttc ttctgggata attgaagctg catcattcaa aataccagta ataaatttag
    92581 gaaccagaca aaatggacga gaacaatcta aaaatattat aaatgcaaat ctaaatgaaa
    92641 atgaaattga aactgctttg aataaaatac aagatcctca gtttataaaa agcttgaaaa
    92701 atgttaagaa catctacggt gacggcaaca gttctagaag aataacaaaa acattgaatc
    92761 aaatattgaa tgatggggaa gatattaagt ggattcaaaa acaaatagct tattaaaata
    92821 tactagtgat ccatacatta ttattggggc tggcggacac gcaaaagtca ttattgaaac
    92881 cttaagatta gaaaataagt taatatatgg attttttgat gataagcaaa ataaaatgca
    92941 agatctgaaa tatcttggga aaataaacga attgaatagc tgggcggata gcaaaaaaaa
    93001 aatctataaa tttattgtag ctattggtga ctgtgtagta agaaaaaaga ttataaaaaa
    93061 attgaatatg ccaagtgagc aatatggaat agctgttcat ccaagtgcta tagtggcaaa
    93121 agaaatttca attggttatg gaactgttat ttttgcgaat gctgtaatta acccagatgc
    93181 tgtaattgga gagcatgcta taataaacac aggtagcatt gttgagcatg attgtagaat
    93241 taataactat gttcatttat ctccaggtgt atgcttagcg ggtggagtac atgtaggtgt
    93301 aggaactcaa ataggaattg gttctcaatg tatccagatg aagaaaattg gatcttggtg
    93361 tatgataggc gcagggagca ctattgttaa ggatattccc tcacattcct tagcttatgg
    93421 caatccagct aaaattaaga aagaaggaat taattttgaa ttctagaatt ttattatcaa
    93481 gtcctcatat gggaggaaca gaagaaaaat atgttgctaa ggcttttaaa gataattgga
    93541 tagcgccatt aggtgaaaat gtggataaat ttgaagaaag tattacaaaa tatactggtt
    93601 ctaagggcgg agcttctgcg ttaagttcag gtacagctgc catacatctt gccttaagac
    93661 tgcttaatgt tggaaaaaac gatattgtct ttgctccttc atttactttt atagctacag
    93721 taaatcctat tttatatcaa ggggctgtac cagtgttcat tgactcagaa cctaatacat
    93781 ggaatatgag ccctgttgca ctagagagcg cattagaaga agctgaaaga aacaaccaat
    93841 tacctaaggc aatcatcgta gtgaatttat atggtcaaat ggctgattat gaacgaattt
    93901 tagctattgc aaaccaattt aatgttccag tgatagaaga tgcagcggaa tcgttaggtt
    93961 ctacttataa gggaatttcg tcaggcttat ttgggacaat tgggatctat tcatttaacg
    94021 gtaataagat catcacaacc tcaggtggag gagctctagt gtctagtgat aaaaaatatg
    94081 ttgagcattc aagattttta gcaacacaag caagagatca agcaccgtat tatcaacata
    94141 gcgaaatagg ttataattac cgtctaagca atatttctgc cggtatcgga cgtggacaaa
    94201 tggaagtatt gaatgataga gtagctaaac gaagagaaat tttcaatcga tataaggaag
    94261 ctttttctgg aattgataga attgaaatga tgccagaagt ggatggcatg gtgccaaatc
    94321 gttggttgtc tactttaacg atcaatgatt ataattctat tgatcaagta gttaacgtaa
    94381 taaataaact aaatgaaaaa aacattgaaa caagagcttt gtggaaacca ctacatttgc
    94441 aaccagtatt tcaaggggta aaaatgtatt accatgataa taatcttagt gtgtcagaaa
    94501 agttatttaa tagcggctta tgcttacctt ctggaagtaa tatgacagat agtgaacaag
    94561 aatttgttat tgatgagttt aaaaaaatat tcaagtaggt gtgatatttt atgaacatag
    94621 gaattattgg tttaggttcc atggggaaaa gaagacttag actcattcaa gaacacttta
    94681 atgatatttt actatgtggt gttgatacta atgaggcgag acgaaaggaa gttgaaaagg
    94741 aatttggaat accaacttat aatagtctaa cagaggcaaa aaatttgttt gaaataaatt
    94801 cagtttttgt ttgtacatcc cctataaccc atgaatttat tataaaagag gctttaaatt
    94861 tagattgtaa tatctttacg gaaatcaatt tattaaatga atattatgat gaaataatcg
    94921 aagtagctaa aaagaaagat agattacttt atctatcttc tacatttttg aagagaaaag
    94981 aaattcaata tattaagtct aaagtgacgc ataataaaaa aatttcttat caataccatg
    95041 taggacagta tttacccgac tggcatcctt gggaagatta tcgcaatttt tttgtttcaa
    95101 acaaaaaaac taatgggtgt agagaaatat tcgctattga attaccttgg attatagata
    95161 catttgggaa agttaaatcg tttactgtaa tgacccagaa attatccaat cttgatattg
    95221 attattcgga cagtttcact cttattattg agcatgaaac tggtatagtt ggaactttaa
    95281 gtgtagatgt tgtttcaaga gtagctaaaa gaaatttaac tataattggt gaaaagattt
    95341 taatagaatg ggaaggtaac ccggattctc tattttcatg gaatgaagaa acttctagta
    95401 tgacagatat tcaattgtac gatgaggtta tgcatgataa taattattca catactatta
    95461 ttgaagatgc atatttagag gagattaaag aatttttcac gcttttggaa aagaaaatgg
    95521 aaccccaata tacttttgaa aaagataagt atattattaa cttaattaac gacattgagg
    95581 taaattaatg atgaaagtaa ttttttttgg gttagggtca attggaaaga gacatctaaa
    95641 aaatctaata agctatggaa aaaagaattc tattaatttt gatataacag cttatagtag
    95701 cagtggaaaa aaagagtttg aagaaataac gtatattcaa aatagagacg atttggatac
    95761 acattatgat atagctttta ttacaaatcc gacttttaag catctggaaa cactaaatgt
    95821 aattgttgaa aaagttgatt acatattttt agaaaaacct gctttttctg agagtattga
    95881 tcttactcaa tataattatg attacaaaaa tatttatgtt gctgctccac tgaggtataa
    95941 aaatatcatg gaagttgttc gtaatagtat tcaaagtaaa aaagtatatt ctgtaagagc
    96001 tatttgttct acttatcttc ctgattggag aaaaggtact gattatagaa ataattattc
    96061 tgcctttaaa tctatggggg gaggagtaga attagactgt atacatgaat tggattatat
    96121 tataaaccta tttggtttcc ctaaatcagt aaaaaaacag tttggtaaat attcgaatct
    96181 tgaaattaac agtaatgatt tagcaacata tatattgaga tacgatgata aaattataga
    96241 aattcattta gattattttg gaagagttcc tacaagaaaa tttcaattgt ttactttaga
    96301 tgaatgtatc aatgttgact tattaaataa taaaataagt tttgaagaaa acattgttaa
    96361 agaaatgcct gaatctgcta atgatatgta tgagaaggag ttgaaatatt tctttgaaaa
    96421 cgtagtaaca ggcaaagaaa actggaatga tttatatcat gcaaacgaag tattgaaaat
    96481 agctgaggag aaataaatgg aaattttatt tactatttgt ggtagagctg ggtctaaggg
    96541 gtttaaaaat aaaaatttga aatatttggc aggaaagccc ctagtccatt tcgctttagc
    96601 aatgatagat atctatcaac aaaaacatcc tgaacataat tgcacgactt gtttgaatac
    96661 ggacagtaat gagttagtag aattgacgac taagttaaat ccgtctgtga aaattgtaga
    96721 acgtaaacct gaattagcta cagatactgt agggaaaatt gaagtgatac gtgattcatt
    96781 cttacaacta gaaagagaag ataatatatt tgatttaatt atagatttag atttgacttc
    96841 acctttacgt gaagtttcta atcttgaaga attaatagat gaacatctaa aaaatttaga
    96901 taaagacgtc tacttttcag ttgtttctgc aagacgtaat ccctatttta atatggtaaa
    96961 aagaattgaa aatgaagtta ctatagttaa taaatcaaga tacacatcta ggcaacagac
    97021 acctgaagtt tttgaaatga atgcctcaat gtattctttt aaacctgaat ttctaaaaaa
    97081 aagtaattat atttttgatg ggcaatgtgg gataataaaa atgagagatt atctaatttt
    97141 agatattgat agtgaagaag attttaaatg gttagaattt ttgtttccta aatttttaga
    97201 agattcttcg gagcttcaag atatctataa ttatttatat cggaggtaaa aggatgagct
    97261 gttatcttat caccggtggg gctggcttca ttggttctac cttagccaat catttaggag
    97321 aagaacatgt ggtaatcgtc gtcgatgact tatcaatggg aaaagtagaa aatcttgata
    97381 tgacgaaaaa tatcactttt attgagggag atgtcgctga gaaatccttg atggaagaga
    97441 tcatgaaagc taataaattt gattatatct ttcatttagc agcagttgct agtgtggcag
    97501 attcagttgc ccgaccagta gaaacacatc gtgtgaattt tgaaagtgta ttaatgattt
    97561 tagaattgat ccgcaagtat caacctgatt taaaacggat cgtgtttagt tcttcagcgg
    97621 ctgtttatgg ggatgaaccg actttaccaa aaaaagaaga gtccgtgatt cgcccattga
    97681 cgccttatgc ggttgataaa ttcgctgcag aacaatatgt gctagattat tgtcatttat
    97741 atgatgtacc cggtagcgca gttcgttttt tcaatgtcta tggtccaaat caaaatccga
    97801 attctccata cagtggtgtg atttctattc tcgtagatcg ctataagaaa caaatcgtag
    97861 gagaagcaac ttcatttaca ttatttggtg atggaagcca gtctagagac tttgtttata
    97921 tagatgatgt aatccaagca ttacttttag ttgcgaatga ggaagctgca ctggggaaac
    97981 aattcaatgt tggaacgggt aaagcaacca cacttttgga attgattcat gcaatcaatg
    98041 aaattctcgg tgtagaattg acacttgaat acaaagaaga gcgttcgggt gatatccatg
    98101 actctctagc agatattact aagatccagt caattggcta tcaaccgaaa tatggtattt
    98161 taagtggaat gactaattat ttgaaaactg aaatcaatta aaacatacta agataagatt
    98221 agagggtaaa ttcaggctct ttgtcaataa ggactgatga gttttgtaaa atcaaatctg
    98281 ggatagaa
//
LOCUS       DACEEZ010000003        96489 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20313_2017
            SAMN12639966-rid9879223.denovo.003, whole genome shotgun sequence.
ACCESSION   DACEEZ010000003 DACEEZ010000000
VERSION     DACEEZ010000003.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639966
            Sequence Read Archive: SRR10023158
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 96489)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 96489)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741683.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741683.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:44:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,016
            CDSs (total)                      :: 2,962
            Genes (coding)                    :: 2,889
            CDSs (with protein)               :: 2,889
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 36 of 73
            Pseudo Genes (incomplete)         :: 29 of 73
            Pseudo Genes (internal stop)      :: 18 of 73
            Pseudo Genes (multiple problems)  :: 8 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..96489
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639966-rid9879223.denovo.003"
                     /isolate="E20313_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            162..392
                     /locus_tag="IWO89_000194"
     CDS             162..392
                     /locus_tag="IWO89_000194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1146 domain-containing protein"
                     /protein_id="HAQ0454984.1"
                     /translation="MQVYGIDAVLRIVSHLAFIYLAFWSLRSLRIETFFRPMHNTQIR
                     MAIVLFSIFLGYTASNFFLEVIALCRNLFVSL"
     gene            489..1790
                     /gene="murA"
                     /locus_tag="IWO89_000195"
     CDS             489..1790
                     /gene="murA"
                     /locus_tag="IWO89_000195"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HAQ0454985.1"
                     /translation="MEEIIVRGGNQLNGTVRIEGAKNAVLPILAASLLAEEGITTLDN
                     VPILSDVFTMNQVIRHLNVDVDFDEQKNQVTIDASRQLEIEAPYEYVSQMRASIVVMG
                     PLLARNGHAKVAMPGGCAIGKRPIDLHLKGFQALGAKIIQKNGYIEAIADELIGNTIY
                     LDFPSVGATQNIMMAAVKAKGTTIIENVAREPEIVDLANILNKMGAQVYGAGTETMRI
                     EGVDHLHAVNHSIVQDRIEAGTFMVAAAMTQGNVLIADAISEHNRPLISKLIEMGAEI
                     IEEEGGVRVIGPKHILPTDVKTMPHPGFPTDMQAQMTAIQLVAEGTSVVTETVFENRF
                     QHLEEMRRMNAHVKIDGNVAIMDGNHELQGAEVYATDLRAAAALVLAGLKANGITRVR
                     NLNYLDRGYYNFHIKLQQLGADVERVDMDQTSAEKTAQTIA"
     gene            1803..1979
                     /locus_tag="IWO89_000196"
     CDS             1803..1979
                     /locus_tag="IWO89_000196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HAQ0454986.1"
                     /translation="MSNQRYILLTLIKILVVILLLILLFVAGTMIGYGVIGGGNPFKV
                     FQPSLWIHIRDFFH"
     gene            2114..2410
                     /gene="yidD"
                     /locus_tag="IWO89_000197"
     CDS             2114..2410
                     /gene="yidD"
                     /locus_tag="IWO89_000197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HAQ0454987.1"
                     /translation="MKKILIYLVRGYQRFISPAFPPSCRYYPTCSNYMIKAIQTHGAL
                     KGTVMGTARILRCHPFVKGGIDYVPLTFQLTRNKKDKKEPDYKKVVKNTSSTER"
     gene            2474..2941
                     /locus_tag="IWO89_000198"
     CDS             2474..2941
                     /locus_tag="IWO89_000198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAQ0454988.1"
                     /translation="MIEELNKKDLPWSLLLDADPDKEKVQVYVSRGSGWIWKEKEKTI
                     GVLIYVAREKEYEIVNVAVAPTYQGKGIGGKLLETAFQKLSQLASSQTQIIIRTGSTS
                     SAALHLYQKMGYVEIGRVKDYFIHHYSEPIFENGDQLRDQVILAIHLPKNSFN"
     gene            3026..3910
                     /locus_tag="IWO89_000199"
     CDS             3026..3910
                     /locus_tag="IWO89_000199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HAQ0454989.1"
                     /translation="MGILVFDFGGSAVKYGCWDGKEIKGKGQFATPESWEEMKAQLFQ
                     VYKKILVSFEVEGIGISSPGVVNNEKQVIEGISAIPYIHGFNIFRDLETLFQLPVTIE
                     NDANCAGMAEFYEGAARDYQDVAFVVAGTGIGGALFTKGQINKGAHLYGGEFGLMFLN
                     GDKTFSKLGTAVQMAWRYCERKGLDKNSYTGKDVFELAETGDAIAKEEVESFYTYLTK
                     GLFSIQFSFDPEVIVLGGGVSAKEGLIDEIKSRMKKLTEQFDLHDFEPQILLCEYRND
                     ANLVGAAANYLAVRQGEN"
     gene            4027..4260
                     /locus_tag="IWO89_000200"
     CDS             4027..4260
                     /locus_tag="IWO89_000200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2969 domain-containing protein"
                     /protein_id="HAQ0454990.1"
                     /translation="MSKKNKDVEVRIEEVKKDIKGKNYEVTQLFIGKKMIGEILAYGP
                     KEYEIFFGEEDFGKEKSLENAIETVIRHWNLHE"
     gene            4348..4428
                     /locus_tag="IWO89_000201"
     tRNA            4348..4428
                     /locus_tag="IWO89_000201"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4382..4384,aa:Tyr,seq:gta)
     gene            4435..4506
                     /locus_tag="IWO89_000202"
     tRNA            4435..4506
                     /locus_tag="IWO89_000202"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4467..4469,aa:Gln,seq:ttg)
     gene            5021..5728
                     /locus_tag="IWO89_000203"
     CDS             5021..5728
                     /locus_tag="IWO89_000203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAQ0454991.1"
                     /translation="MSNQLPVYIQIHDQLKNEIEQGIWKVGDRLPSERELAVKFNVSR
                     MTLRQAIQNLADEGILERKIGSGTYVAREKVQEKMSGATSFTEIMESQNRVPSSRTIS
                     YFLTSPSSSEMEKLNLSKDDTILRMERIRFADDVPICFEVASVPQKLIDGYSKAEITE
                     SLYRTLEEKGGKKIGPANQTITAVLASEKIADYLEVKRGDAVLRLRQISYFEDGTPFE
                     YVRTQYVGNRFEFYLEK"
     gene            complement(5788..6432)
                     /locus_tag="IWO89_000204"
     CDS             complement(5788..6432)
                     /locus_tag="IWO89_000204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YigZ family protein"
                     /protein_id="HAQ0454992.1"
                     /translation="MIERYRTIKENGQSEIEIKKSRFICSLRRIESEDQAKSFIQEIK
                     KEHWKANHNCSAFIIGEKNEIQRSSDDGEPSGTAGVPMLEVLKKNQLINVAAVVTRYF
                     GGTKLGTGGLIRAYTHAVSQALSDIGIVEGTLQQEISVMITYPQLGKLQNYLELQHFF
                     IKEIQYTDQVSVICAVDEKSADHFKESIIELLNGQVQFEEGPVSYHEQLVTDHI"
     gene            6559..6846
                     /locus_tag="IWO89_000205"
     CDS             6559..6846
                     /locus_tag="IWO89_000205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0454993.1"
                     /translation="MQERMKKYDAITHYLKNNGGSQVTLTFTQFDELLFPSNGLPKTA
                     RESTDWWANDYKHPEKGAYGWINAGYEVVVINLDKEYVVFNKLVKSSWLFD"
     gene            6954..7274
                     /locus_tag="IWO89_000206"
     CDS             6954..7274
                     /locus_tag="IWO89_000206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAQ0454994.1"
                     /translation="MAKKTIMLVCSAGMSTSLLVTKMQKAAEAKGIDSDIFAVSASDA
                     DNNLANKDVDVLLLGPQVRFMKADFEKRLEPKGIPLDVINMADYGMMNGEKVLDQAIT
                     LIGE"
     gene            7355..8668
                     /locus_tag="IWO89_000207"
     CDS             7355..8668
                     /locus_tag="IWO89_000207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HAQ0454995.1"
                     /translation="MNHFWGRRLLTRDIKAIDCEEVNLPNYEKIAAVERLGNWILCHR
                     CGMRTSVIDGQLADYGYFCTHCLYLGRCDTQQDLYLFDQPEAKPREVVFFWTGQLTEK
                     QTEIAKRILCHSEKRHHLIWAVTGAGKTEMLYPILVETLKAGGRVAICTPRVDVCNEL
                     FLRYRYVFPEEKITLLHGNTATAYTFTSFVICTIHQLYRFYRSFDLIVIDEIDAFPYN
                     GDAGLAHAVQTAIKPDGRFIYLSATPDKKLLKEIKQTFDIHKLALRFHRRLLPEPVLV
                     FMNNWRQKCKNKKKIKSLVRIIHQLIKENHILLFCPSITMMHRLKATLQEALPRYEIT
                     DVSSRDNQRAEKVQKMREAKYDILLTTMILERGVTFERISVIVLGADHPVFTKSSLVQ
                     IAGRADRKGPYTNSQVYFFYEEKTAAIRKACQEIKEMNEEGKKML"
     gene            8665..9384
                     /locus_tag="IWO89_000208"
     CDS             8665..9384
                     /locus_tag="IWO89_000208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComF family protein"
                     /protein_id="HAQ0454996.1"
                     /translation="MRCSCCSKDIVQNLTFSEIIGVSKYRLPQTCSVCRAQFHLLETR
                     GCSGCQKPIHFNLESTKKKVKSIPVYCSDCQEWKKKYPTYDFQHRAFFVYDENMKAWL
                     NQYKFMGDIRLAGTFAYKWTQIKKEYPTYIYCPIPLSEKRRTERGFNQVSEMLKAARI
                     PFQMLLKREKHLLAQAKNTRKERLEMPQPFELAVTFSKIQNKKILIIDDVYTTGQTLF
                     HAADCLQVAQPKIIRTISLAR"
     gene            9512..10063
                     /gene="raiA"
                     /locus_tag="IWO89_000209"
     CDS             9512..10063
                     /gene="raiA"
                     /locus_tag="IWO89_000209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated translation inhibitor RaiA"
                     /protein_id="HAQ0454997.1"
                     /translation="MFRYNVRGENIEVTEAIRDYVEKKVGKLERYFSDAPDATAHVNL
                     KVYTEKTAKVEVTIPLPYLVLRAEETSPDLYASIDLVVDKLERQIRKFKTKINRKSRE
                     TGMNTADATALFNEEDENENDSELDIVRTKRLSLKPMDSEEAVLQMNMLGHNFFIFED
                     AETNGTSIVYRRKDGKYGLIETD"
     gene            10285..12819
                     /gene="secA"
                     /locus_tag="IWO89_000210"
     CDS             10285..12819
                     /gene="secA"
                     /locus_tag="IWO89_000210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010718571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HAQ0454998.1"
                     /translation="MANLIRSLIENDKKELKRLDSIAKKVEAYADQMAALTDEQLQAK
                     TAEFKGRYQKGETLDQLLPEAFAVVREAAKRVLGLYPHHVQLMGGIVLHDGNIPEMRT
                     GEGKTLTATMPVYLNALTGEGVHVVTVNEYLATRDSTEMGELYNFLGLTVGLNINSKS
                     SDEKREAYNCDITYSTNNELGFDYLRDNMVVYRHQMVQRPLNYAIVDEVDSILIDEAR
                     TPLIISGQAEKSTALYTRADNFVKRLKEEEDYKIDVQSKTISLTEAGIEKAEETFGLD
                     NLYDIENTALTHHLDQALRANYIMLLDIDYVVQDGKVLIVDQFTGRIMDGRRYSDGLH
                     QAIEAKEGVEIEDETKTMATITFQNYFRMYKKLAGMTGTAKTEEEEFREIYNIQVYQI
                     PTNRPVIRDDRPDLLYPTLQSKFHAVVQDIKDRYRKGQPVLVGTVAVETSELLSDMLN
                     QERIPHEVLNAKNHFKEAEIIMNAGQKGAVTIATNMAGRGTDIKLGLGVRELGGLAVI
                     GTERHESRRIDNQLRGRAGRQGDPGMSQFYLSLEDDLMKRFGSERIKSFLDRMKIGEE
                     DAVIQSKMLTKQVESAQKRVEGNNYDTRKNVLQYDDVMREQREVIYKQRQEVIMEEKS
                     LSKPLMNMVKRTISRVVDAHTQLEKENWNLESIIDFAGSTLVHEDSITLADIEGKTAQ
                     EIKEYLNERAQEVFNTKAAQLNGPEQLLEFEKVVILRVVDSKWTDHIDAMDQLRQSIG
                     LRAYGQNNPLVEYQTEGYKMFEDMVGAIEYEVTRIFMKAEIRQNVQREQVSQGTASHS
                     EDGDAKENSNTSAKKQPIHVNKVGRNDPCPCGSGKKYKNCHGKNAN"
     gene            12945..14046
                     /gene="prfB"
                     /locus_tag="IWO89_000211"
     CDS             join(12945..13007,13009..14046)
                     /gene="prfB"
                     /locus_tag="IWO89_000211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_078989099.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HAQ0454999.1"
                     /translation="MEISEIRNLLTEMQEKVTSFRGLFDLDQLEEDIAEAEAKMAEPG
                     FWDDSEAAQKVISENNANKETYDQFHSLAEELEEIELMVEMLQEEPDAAMQEEAETRI
                     NALDEKMKTYELSMLLDGPYDRNNAIVELHPGAGGTESQDWGSMLLRMYTRWAEQHGY
                     QVETLDYQAGDEAGIKSVTLLIKGHNAFGYLKSEKGVHRLVRISPFDSAKRRHTSFCS
                     VDVMPELDETIDIEINPDDLKIDTYRASGAGGQHINKTESAVRITHLPTGTVVASQAQ
                     RSQLKNREQAMGMLKAKLYQLEVEKKEQEAAALRGEQKEIGWGSQIRSYVFHPYSMVK
                     DHRTNVETGNVQAVMDGDLDLFIDGYLKMHLQ"
     gene            14151..14837
                     /gene="ftsE"
                     /locus_tag="IWO89_000212"
     CDS             14151..14837
                     /gene="ftsE"
                     /locus_tag="IWO89_000212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005237293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division ATP-binding protein FtsE"
                     /protein_id="HAQ0455000.1"
                     /translation="MIEMKDVMKKYSNGTTAIRNLSIVIEQGEFVYVVGPSGAGKSTF
                     IKLMYREEKASKGTLNVAGHDLIAIKNREVPYLRREIGVVFQDYKLLPRKTVYENVAY
                     AMQVIGRKPRDIKKRVMEVLDLVGLKHKVRVFPSELSGGEQQRVSIARAIVNTPKVLI
                     ADEPTGNLDPENSWEIMKLLDRINAQGTTVVMATHNSTIVNTIRHRVIAIENGRIIRD
                     QAEGEYGYDD"
     gene            14821..15714
                     /locus_tag="IWO89_000213"
     CDS             14821..15714
                     /locus_tag="IWO89_000213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAQ0455001.1"
                     /translation="MDTMIRTFFRHLLESIKSLKRNGWMTVASVSAVTITLTLVGVFM
                     AVIMNATKLAQDIEGNVDVSVFVDIGTKDEEMKKLEKELNDLDHVKKVEFSSKEQELK
                     KIQNEMGDSWKLFEGDNNPLYDVYVVSATDPSYTKQVAEEAADLKNVSRSDYGGASSD
                     RIFQIAGAVRTWGLVAAALLLFVAMFLISNTIRITILSRQREIQIMRLVGAKNGYIRW
                     PFFLEGGWIGLIGAILPILIITFGYAWFFQLINPSLLRSHYSLIHPQNIVWKINLLMV
                     GIGVIIGSLGSIISMRRFLKF"
     gene            15866..17314
                     /gene="cls"
                     /locus_tag="IWO89_000214"
     CDS             15866..17314
                     /gene="cls"
                     /locus_tag="IWO89_000214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase"
                     /protein_id="HAQ0455002.1"
                     /translation="MKIIVDNFFTILLIMNILLSFIIVFRERKETAQTWAWVLVLMFI
                     PVVGFILYIFLGRGISKEKIFDLKIQGKIGKNLEIEEDKQALMRGLYPHPPTGQVDVK
                     QLIYMLTVFESTLYTTKNEITLFTDGREKFDALIEDIKQAEDHIHFQYYIYRSDALGE
                     EVRDALTEAARRGVKVRVLLDAWGSTQVSSSFFQNLKKAGGEVAFFFPLFVPYINPRI
                     NYRNHRKIVVIDGKIGYTGGFNVGNEYLGLVKKFGYWRDNHLRIYGEAVYTLQNRFLM
                     DWNSQHTKEVGYSPKFFPSIHSTGDIAVQIVTSGPDTEHEQIKMTYLKMISLAKREIL
                     IQTPYYIPDGSIHEALKLALLSGVKVHIQIPNKPDHLLVYWATYSFAAELIEYGARIE
                     TYENGFIHAKTMIIDGGIVSVGSANIDVRSFRLDFEVNTLVYDERMASRVRKAFFEDS
                     KISTHLTKEMYENRGIIIKMKEGLARLISPLL"
     gene            17651..18508
                     /locus_tag="IWO89_000215"
     CDS             17651..18508
                     /locus_tag="IWO89_000215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein PstS"
                     /protein_id="HAQ0455003.1"
                     /translation="MKKLKLFLPIIGLAILLSGCSKWIDRGESITAVGSSALQPLVET
                     VAETYQTENPGKFINVQGGGSGTGLSQVQSGAVDIGNSDLFAEEKSGIDASALVDHRV
                     AVVGITPIVNKDIGVKDISMENLKKLFTGEIKNWSEVGGKDQPVVILNRASGSGTRST
                     FEKWVLDGETAVQAQEQDSSGMVRQIVADTPGAISYVAFSYVSDDVDTLNVNGVEPTD
                     ENVTTNKWTIWAYEHMYTRDHPKKLTKEFLDYILSDEVQDNIVGELGYIPVSHMKVER
                     DWQGNIVKE"
     gene            18623..19543
                     /gene="pstC"
                     /locus_tag="IWO89_000216"
     CDS             18623..19543
                     /gene="pstC"
                     /locus_tag="IWO89_000216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002361932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease subunit PstC"
                     /protein_id="HAQ0455004.1"
                     /translation="MEDVKKVLLTKSKRSKMEQRGKFISFLCIALIVLVVLSIFYFVA
                     SKGLATFFTDKINVFDFLFGTDWNPSQIGANGKPMVGALPMITGSFIVTFLSAIVATP
                     FAIGAAVFMTEISPKNGTKILQPVIELLVGIPSVVYGFIGLSVIVPFVRSIFGGTGFG
                     ILAGTFVLFVMILPTVTTMTVDALKAVPRHYREASLALGATRWQTIYKVVLRAAVPGI
                     LTAVVFGMARAFGEALAIQMVIGNAALMPTGLTTPASTLTSILTMGIGNTIMGTVENN
                     VLWSLALILLLMSLFFNIVIRMIGKKGELK"
     gene            19543..20427
                     /gene="pstA"
                     /locus_tag="IWO89_000217"
     CDS             19543..20427
                     /gene="pstA"
                     /locus_tag="IWO89_000217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010764435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HAQ0455005.1"
                     /translation="MNAKRSDKIATGVLYIVSGIIVLILAALLLYILVRGIPHISWSF
                     LTEPSKAYQEGGGIGIQLFNSIYLLLITMIISFPISLGAGIYLSEYAGKNWLTDVIRT
                     SIEILSSLPSVVVGLFGFLVFVIQFQYGFSIISGALALTVFNLPLLTRTVEESLQAVH
                     YTQREAGLALGLSRWETVIKVVVPEATPGILTGVILSSGRIFGEAAALIYTAGQSAPA
                     LDFTNWNPLSVSSPISIFRQAETLAVHIWKINTEGTMPDGVSVSAGASAVLIIAVLLF
                     NFGARAIGKRLYRKMTSA"
     gene            20442..21248
                     /gene="pstB"
                     /locus_tag="IWO89_000218"
     CDS             20442..21248
                     /gene="pstB"
                     /locus_tag="IWO89_000218"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein"
                     /protein_id="HAQ0455006.1"
                     /translation="MKKYNWNETNVITMPKEKEVALYTDDLHVWYGDNEAIKGVDLEF
                     EKNKITALIGPSGCGKSTYLRSLNRMNDGIANTKITGKIMYQGVDINAPQVDVYEMRK
                     RIGMVFQRPNPFSKSIYDNITFALKQHGEKNKKKLDEIVETSLKQAALWDQVKDSLDK
                     SALALSGGQQQRLCIARAIAMKPDILLLDEPASALDPISTGTVEETLVNLKEDYTIII
                     VTHNMQQAARISDYTAFFYLGKVMEYDKTNKIFTRPKIQATEDYVSGHFG"
     gene            21264..22022
                     /locus_tag="IWO89_000219"
     CDS             21264..22022
                     /locus_tag="IWO89_000219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein"
                     /protein_id="HAQ0455007.1"
                     /translation="MTKEIISSKDLHLYYGKKEALKGIDLSFNQGEITAMIGPSGCGK
                     STYLRSLNRMNDLIPNVTITGSVLYKDKDIYGPKTDVVELRKEIGMVFQQPNPFPFSI
                     YENVIYGLKLKGEKDKKVLDQVVEESLKAASVWDDVKDKLHKSALSLSGGQQQRVCIA
                     RVLAVNPEIILLDEPTSALDPVSTGKIESMLLELRERYTMIIVTHNMSQASRISDKTA
                     FFLDGHLIEYNDTKKIFMNPEKQETEDYISGRFG"
     gene            22035..22712
                     /gene="phoU"
                     /locus_tag="IWO89_000220"
     CDS             22035..22712
                     /gene="phoU"
                     /locus_tag="IWO89_000220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010723432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate signaling complex protein PhoU"
                     /protein_id="HAQ0455008.1"
                     /translation="MLRTQFEEELLNLHNQFYEMGMMVSSAVHKSVRAYIKHDKILAQ
                     EVIENDININNMETRLEKKSFEMIALQQPVTTDLRMIITVMKASSDLERMADHAVSVA
                     KSTIRVKGQTRIPEIEKEISDMSDYVKKMVDNVLVAYVKTDEKDARTIANMDQRVNDY
                     FNRIYNATIKNMQENPETVISGTDYLNVASYLERIGDYVTNICEWIVYLATGKITELN
                     TNHNEIF"
     gene            22950..23981
                     /locus_tag="IWO89_000221"
     CDS             22950..23981
                     /locus_tag="IWO89_000221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YibE/F family protein"
                     /protein_id="HAQ0455009.1"
                     /translation="MQKKISIITIFFICVLTSWVIQHQAALYEVPVIEIEKVRIKEDH
                     QLITGKLMNTDQKGESIYLSVPYQKNQIETANIKARQMYLVQMHGDEWQLVAKKNDGW
                     LFFFTSIFIGAMLLIGGKQGLFALGSLIINIVLLLLLLLIFTKTEGISLLNISILFVV
                     IGMIISILALDGWNRSSIIKISAAVTSVFLAFFICLLTMNHWKDQGLRYEELNYLTRP
                     YRSVFLSSVLIGTAGGTLDTVITVIATLEELTKRQPEITAKQLWLSGRQVGRDVIGSM
                     TNILLFVYISGSVPSLLLYLGNQWSFKETIEQHLSLEFLRVIAGSLGIVLSIPISVLF
                     FLTVRRLKR"
     gene            23978..24745
                     /locus_tag="IWO89_000222"
     CDS             23978..24745
                     /locus_tag="IWO89_000222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YibE/F family protein"
                     /protein_id="HAQ0455010.1"
                     /translation="MNVLIILVGILAISVLLIGGTQGMYILLGLFINLGIFFLLLFGY
                     HQKWPILALSVIGFLLIAVVILFFINGYNLKMRAAFVSILIFLFCFLLLLPITDFLAI
                     QGFTSVELEELSGLDKTLAIDFRLLTRSLLLISLSGAVLDASVAISSGTFEVYQANPQ
                     LSFYQLRHSSFAIAKKILASTVMTLLFAFMGSSLALILWFIDLDIPFQQVINEKSFVL
                     EYTMAILTTLSALLVLPLTCVTTAYLFTKKKEQLKME"
     gene            24845..26413
                     /gene="liaX"
                     /locus_tag="IWO89_000223"
     CDS             24845..26413
                     /gene="liaX"
                     /locus_tag="IWO89_000223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="daptomycin-sensing surface protein LiaX"
                     /protein_id="HAQ0455011.1"
                     /translation="MKERERILDLVKKGILSTEEGLDLLESMATEKDEKQIKKEADKV
                     TASHKEKDQASQLIDKLENGEEEISEPVDPKERERQDQENLEKILDELATEANKTSAR
                     LDEVNAQFADNKAARNEKQEALMQLNTKEELGELTEEELAQRQTLEAEIKELEASGDT
                     LLEEQLKLEAELKDIRKNQWSEKKETFTDKFELPDDWKDQATDTLNQVGEKMSEAGSQ
                     LGKFLKKTFQTVSETVNDNMEWKDVSLRVPGIATTKFEHEFYYEAPAASILDIKAANG
                     NVTLKTWDSNDVKVEAKIKLYGKMGAEPFEAFSERSQIEVNEDHISFQIPNKRVRADL
                     VFYLPKRVYDHAAIKLLNGNIMIETLEAKDIYTKSTNGNIIVDQLTATMLEVEGVNGN
                     IDIRNGNILDSIIETVNGTVTFGATAENLSVSLVNGDVRLTIKEDNLKKVEASSVNGN
                     VKVALPDGIGLEGHAKTSLGSINSRLSNYEVVREKKERTNQMLQFRRVSDGEIAQVQL
                     STTTGSIYLKDTDK"
     gene            26713..27030
                     /locus_tag="IWO89_000224"
     CDS             26713..27030
                     /locus_tag="IWO89_000224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PspC domain-containing protein"
                     /protein_id="HAQ0455012.1"
                     /translation="MKKKLTKSNKNVVLTGTLAGIGEYIGIDPTVIRVLYVFLSLVAF
                     GSPIILYILLALIIPSARSARTNYGHNNAYYEKNNYREQTREQTKKRKEAEKIDDDEW
                     SDF"
     gene            27030..27380
                     /locus_tag="IWO89_000225"
     CDS             27030..27380
                     /locus_tag="IWO89_000225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HAQ0455013.1"
                     /translation="MSYFQRILVNTLTFISLAVLFPAMVHVSSIWTAVLASFVLSVLN
                     MFVKPILTILSLPFTLVTLGLFTFVINAAILKMTSFFVGAEHFSFSSFGAALLVAILM
                     SLVNVIVSEHNSRK"
     gene            27547..28485
                     /locus_tag="IWO89_000226"
     CDS             27547..28485
                     /locus_tag="IWO89_000226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HPr kinase/phosphorylase"
                     /protein_id="HAQ0455014.1"
                     /translation="MSEVVKVSQLVKKLSLEIVTGDEKSLDRVIVTGDISRPGLELTG
                     YFNYYSHNRLQLFGSKEISFAERMIPEERLIIMRRLCSEDMPAFIISRGLPAPEEMIQ
                     AANENGIAVLRSPISTSRLLGELSSYLDGRLAPRTSVHGVLVDVYGLGVLIQGDSGIG
                     KSETALELIKRGHRLIADDRVDVYQQDELTVIGEPPKILEHLIEIRGIGIIDVMNLFG
                     ASAVRGSMQVQLAVYLEAWAKDKKYDRLGSEDTTVEIAEVGIPQIKIPVKTGRNVAII
                     IEVAAMNFRARTMGFDATKTFEERLSRLIEENSGND"
     gene            28500..29330
                     /locus_tag="IWO89_000227"
     CDS             28500..29330
                     /locus_tag="IWO89_000227"
                     /EC_number="2.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HAQ0455015.1"
                     /translation="MLAQINRVAFELVGIPIYWYALIIVSGIIIAMWLSSREAVRVGL
                     KADDVTDFMLWGLPISIIGARLYYVLFDLPQYIADPIQIFNIRSGGLAIYGGLIAGAI
                     TLYFFTKYHFISLWTFLDIAAPSVLLAQAIGRWGNFMNHEAYGPDTTRSFLESLHLPQ
                     FIIDNMYIEGIYRQPTFLYESLWSLIGFILLLFLRKKPHLLKKGEVLLCYLMWYSFGR
                     FFIEGLRTDSLYFLGVIRISQLLSAVLFVGTLILFIWRRKKGNLPDYDRSLGKNQQII
                     "
     gene            29374..30399
                     /locus_tag="IWO89_000228"
     CDS             29374..30399
                     /locus_tag="IWO89_000228"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002363959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HAQ0455016.1"
                     /translation="MNYRVAVLGPGSWGTALAQVLAENGHDVRIWGHRASQVEEINTQ
                     HTNQRYLPDHYLPDSIQAFTNMEDAVKDADAVLFVIPTKAIRSVAKELVPKLNTKPVI
                     IHASKGLEQGTHKRISDILLEEIPAEKRKAVVVLSGPSHAEEVAKHDITTITAASTDA
                     KAAKYVQELFMNNYFRIYTNPDVIGVEMGAALKNIIAIGAGAIHGLGFGDNAKAAIMT
                     RGLAEISRLGVAMGANPLTFIGLSGIGDLVVTCTSVHSRNWRAGNLLGQGQPLEEVLA
                     NMGMVVEGVNTTQAAMELAQMMEIEMPITQTIYEVLYEQKEIKTAAKEIMLRDGKKEN
                     EFSLTIF"
     gene            30414..31352
                     /gene="galU"
                     /locus_tag="IWO89_000229"
     CDS             30414..31352
                     /gene="galU"
                     /locus_tag="IWO89_000229"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HAQ0455017.1"
                     /translation="MKVKKAVIPAAGLGTRFLPATKAMAKEMLPIVDKPTIQFIVEEA
                     LKSGIEDILIVTGKAKRPIEDHFDSNIELETNLSEKGKTELLKLVEETTDVNLHFIRQ
                     SHPKGLGHAVLQARAFIGNEPFVVMLGDDIMEDEVPLTQQLIDDYERTHASTIAVMQV
                     PHDETSKYGIINPGEVLEDGLYNVKNFVEKPDPSKAPSDLAIIGRYLLTPEIFDVLSE
                     QQPGAGNEIQLTDAIDTLNKTQRVFARKFTGKRYDVGDKFGFMKTSIEYGLTHPEVGA
                     PLREYILSLGKTLEAEGSKIDHNPKGITEIKEEKKK"
     gene            31477..31917
                     /locus_tag="IWO89_000230"
     CDS             31477..31917
                     /locus_tag="IWO89_000230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF948 domain-containing protein"
                     /protein_id="HAQ0455018.1"
                     /translation="MSGGEIAGLIAAIAFAILVVFIVRVLLQVSKTLEKVDQTVAEAN
                     TTIEIVTKDVDLLSRQVEGLLVKSNELLLDINGKVATIDPLFTAVADLSESVSELNHS
                     SKNIAPKVGGIGKTTAKATVAGKVGGTAMKFFKNKKHTETTGGK"
     gene            31920..32522
                     /locus_tag="IWO89_000231"
     CDS             31920..32522
                     /locus_tag="IWO89_000231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HAQ0455019.1"
                     /translation="MAKKGGFLLGAVVGGTAAAIAALLLAPKSGKELRDELAAQADDL
                     MDMASDYTDMAKEKSTELTGIAKEKAQDLSQQAGDLAGSVKQKVSESADEAGSESDDI
                     LLNLKEQSADLSNRFKKTAEDMKKPADELGSIAKDTSEDIMIDVKDAAGKAGDTVNEG
                     MSEAKPVADDSKEMMKMAKEDAKEAKKEIKDTMTDSSTNH"
     gene            complement(32581..33687)
                     /locus_tag="IWO89_000232"
     CDS             complement(32581..33687)
                     /locus_tag="IWO89_000232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HAQ0455020.1"
                     /translation="MNNVKINELRQWMAQHQVELAYISDPGHIAYFSGYESDPHERVL
                     ALFIPLEQDPFLFTPALEVEDAQNSSWAYDVRGYLDSENPWEIIGKELRARYGQPGNV
                     GIEKNALTVERFDAISDILTGTDSYTDLTSIIQRLQLIKTLEEKDKLIEAGKWADVAF
                     EIGFKAVKEGVTEQAIIAEIEYQLKKQGVSQMSFDTLVLAGANAASPHGTPGNTKISP
                     NELVLFDLGVVWNGYCSDATRTIAYQKPTDFQEKIYNITLEAQLAAQEAVRPGVTAGE
                     LDQIARNVINSYGYGEYFNHRLGHGIGTTVHEFPSLVEGNDLVIEEGMCFSLEPGIYI
                     PEKVGVRIEDCVYVTSDGCVPFTTTPKELLVLEK"
     gene            33897..34898
                     /gene="ccpA"
                     /locus_tag="IWO89_000233"
     CDS             33897..34898
                     /gene="ccpA"
                     /locus_tag="IWO89_000233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="catabolite control protein A"
                     /protein_id="HAQ0455021.1"
                     /translation="MEKQTITIYDVAREANVSMATVSRVVNGNPNVKPATRKKVLEVI
                     DRLDYRPNAVARGLASKKTTTVGVIIPDVSNMFFASLARGIDDVATMYKYNIILANSD
                     GNDQKEVNVLNNLLAKQVDGVIFMGHHITDEIRGEFSRSKTPVVLAGSIDPDEQVGSV
                     NIDYVSATKDAVNKLAKSGNKKIAFVSGALIDPINGQNRLKGYKEALSENNLPYSEGL
                     IFEAQYNFKDGLSLVDRIHNSGATAVYVSDDELAIGILDGLLDRGVKVPEEFEIITSN
                     NSLLTEVARPRLTSITQPLYDLGAVSMRLLTKMMNKEEVEEKTIILPYGIEEKGSTK"
     gene            complement(35235..37580)
                     /locus_tag="IWO89_000234"
     CDS             complement(35235..37580)
                     /locus_tag="IWO89_000234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein"
                     /protein_id="HAQ0455022.1"
                     /translation="MPRKNTRKKQNRRKKEKWFVPKIIFRVFQSLTVFITVLLLMFAA
                     LGIGIGAGYFAYLVEDTQLPTKKALQTELGNITETSKIVYADNTEISKIQTDLMRTTI
                     SSDKISPLLKTAIISTEDEYFDKHQGYVPKAVLRALVSEATGIGSSGGSTLTQQLVKQ
                     QILTDETTFKRKANEIILSAQVEKYFSKDEIIATYLNVSPFGRNNKGQNIAGVQEAAK
                     GIFGVDAKDVTLPQAAYIAGLPQSPITYSPYTNTGALKEDLSAGLARKDFVLFSMYRE
                     GQITKEQYEEAKAYDLTKDFLPQQIAEQTDREFLYYTVMNEATRIIAQQLAEKDNADM
                     SDSSVSDAYYQKAQQTIQNKGYTIHSTIDKDVYAALQNGVENYGYMLDDGAGSQVETG
                     NVLMDNRTGRIYGFVGGRNYSQNQNNHAFDTERQAGSSIKPVLVYGPAIDMGLIGSES
                     RVSDYATTWQEGENAGEKIVNATNEGSNTFQTVRESLEWSNNIPAYHLYQDVLNSGGS
                     KQYAYEHYLAKMNYPANDNWGVESAPLGTVDVTTLQQTNGFQALANGGVYEEGYIIDS
                     ITDNAGNVIYKHESNSVRIYSEATASIMNDMMRSVINAKITTPFKDAISSLNGNLGKA
                     DWVGKTGSTNEYRDSWLIVSTPSITISSWAGHDDNTGMDSKARIRSANYLANLINQAY
                     QAKPDIFGTDEKFELSSDVMKKKVSGFTGQTPGKVTVNKKSIQTPGKTVESLWAKNGP
                     KKSAFKFGVGGTDENYTDYWNTASAYARANQATKESDEKDD"
     gene            37859..38761
                     /locus_tag="IWO89_000235"
     CDS             37859..38761
                     /locus_tag="IWO89_000235"
                     /EC_number="3.1.21.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease IV"
                     /protein_id="HAQ0455023.1"
                     /translation="MLLGSHVSMSGKKMLLGAAEEAASYGSSTFMIYTGAPQNTRRKA
                     IEEMNIDAGKAYMAEKGLTNIVVHAPYIINLGNTIKPEMFPFAVQFLREEIVRAEALG
                     ATQITLHPGAHVGAGTEAGIARIIEGLNEVLTKEQTAQIALETMAGKGTEIGRTFEEL
                     AQIIDGVKLNEKLSVTFDTCHTNDAGYNIKEDFSGVMEEFDKIIGLDRLKVLHINDSK
                     NPQGSHKDRHANIGFGTIGFDTLNKIVHDPMFDNISKILETPYVGKDKKKAYAPYGKE
                     IAMFKAQTFDPEVFPELLAEEVIL"
     gene            38974..40749
                     /locus_tag="IWO89_000236"
     CDS             38974..40749
                     /locus_tag="IWO89_000236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosyltransferase"
                     /protein_id="HAQ0455024.1"
                     /translation="MLNTLKPLINQLKKKHTYFILGIALLFTIIRLYLYSKAIYGTDM
                     SATYDDQLLIRYAQNILDGKWLGEYTTTTLSKGISYSLFLVLAHKLHLSYAVLFGMFS
                     IFASILVALAFKPIVKNKYLLLSIYFYFLFSPISFTHEYSTRTYRNALVIPAVEIIIA
                     CLLAIYYRKYGSSRKLLPWFLGLSVAFPFFWFIREDSIWLLPFTVVALGISFVQILLG
                     QDFSFKEVKKLKKRLFLLSKREWLKLSLFLLPIVGFSIVYQWIEYKNETTYGIATIND
                     RSSGEFGRLMKQLIRIDDETNLNKENSEIWVSHAALNKALAASPTFHTVSTRIDELYT
                     THAWTQGGKVKELPGDIIFWALRDVVSESGYYQNNAKETNDFWKKVNQELEEAYHKGT
                     IKKKKEFYLMGSGDGKTMSDLPIVFDFLQSGYAYAWRYKDFHQGGNYSFGTQEQVKQA
                     QELLNLPLMENWIDSNEPRTPNFQLTKSAKVANVIIKIYQVLAIPVLIASIAGILLMI
                     LGTVFAKADRAYYSSSLIVVTGLILTQLVFLFGVSWFCSYAPEKKDYFLSVYTGAGVP
                     LTQFIVVTSMLVFSKLNLKKALSSK"
     gene            complement(40815..42401)
                     /locus_tag="IWO89_000237"
     CDS             complement(40815..42401)
                     /locus_tag="IWO89_000237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="divalent metal cation transporter"
                     /protein_id="HAQ0455025.1"
                     /translation="MNNQSNKQFHKHRLIEHTNGLSLAEVNGTVDVPKGKSFFKTLLA
                     YSGPGALVAVGYMDPGNWSTSITGGQNFQYLLMSVILMSSLIAMLLQYMAAKLGIVSQ
                     MDLAQAIRARTSKTLGVILWILTELAIMATDIAEVIGAAIALYLLFDIPLVIAVFITV
                     LDVFVLLLLTKVGFRKIEALVVCLIFVILFVFVYQVGLSNPDWHSLFAGFIPNSHTFS
                     DSPVIGGETPLTGALGIIGATVMPHNLYLHSAVSQTRKIDHQNEEDVAQAVRFSTWDS
                     NIQLSMAFLVNALLLIMGVAVFKTGSVQDPSFFGLYEALSDPSAMSNGILIAVAKSGI
                     LSTLFAVALLASGQNSTITGTLTGQVIMEGFIHMRIPIWLRRLVTRLLSVIPVLICVL
                     FTSTKGTIEEHAALNNLMNESQVFLAFALPFSMIPLLMMTNSQAEMGSRFKNSFIVKL
                     LGWFSVISLTYLNLRGLPGQIESFFGEQATSAQLALADHIAYVIIVLVLLLLAWTIGD
                     LYKGNKKYEQLLQETVEEIE"
     gene            42652..42876
                     /locus_tag="IWO89_000238"
     CDS             42652..42876
                     /locus_tag="IWO89_000238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YneF family protein"
                     /protein_id="HAQ0455026.1"
                     /translation="MLSTGIVVLIAIIALLIGAVGGFFLARKYMKDYLEKNPPVNEDM
                     LRSMMMSMGQKPSEKKIRQMMQQMKNQGKK"
     gene            43018..44769
                     /locus_tag="IWO89_000239"
     CDS             43018..44769
                     /locus_tag="IWO89_000239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAQ0455027.1"
                     /translation="MSIFRKLGWFFRQEKKHYLIGVFSLFAVALVQLVPPKVIGIIID
                     EIADKNLSIKIIFGWIFLLIIAAFLQYLFRYIWRTNIWGSAARLEKELRQQLFKHFTE
                     MDNLFYQKYRTGDLMAHATNDLNAIQNVAGAGILTFADSVITGGTTIIAMILFIDWRL
                     TLIALIPLPLLAVTSRVLGSKLHDAFRDSQEAFSSINDKTQESITGIKVIKTFGQEKE
                     DLADFEEKIEDAIIKNKRVNFLDSLFDPFITLIIGLSYVATIILGGKYVLEGTISIGQ
                     MVSFISYIGMLVWPMFAIGRLFNVLERGNASYDRVNELLHEKTHIVEQLGAVQTPAKG
                     ELNVAISSFNYPKEESPALEQIKFAIQEGETLGIVGKTGAGKTTIIKLLMREYDDYQG
                     KIQFGGRSIKEYSLNALLGSIGYVPQDHFLFSMTIRDNIRFSNPKLSEEEVEHAASLA
                     FINNEIKELPKGYDTLVGERGVSLSGGQKQRISIARALIKNPELLILDDALSAVDAKT
                     EEAILHNLKEIRKEKTTIIAAHRLSSVMHAKEIIVVDQGKIIERGTHHELLALGGWYE
                     QMWEKQQLEAKIEGGEA"
     gene            44769..46553
                     /locus_tag="IWO89_000240"
     CDS             44769..46553
                     /locus_tag="IWO89_000240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ0455028.1"
                     /translation="MDENYQSEWTKSIPLKEQKKIIKRLMKFAKPFRRTFIVAILFAF
                     ALSVINVLLPRIIQTFMDDHLAKQSATTQVILFFAGMYLFGVIVKSIIWFFQWYLYSM
                     ASLKTYQYIRVKLFEKLHTLGMRYFDQTPAGSTVSRVTNDTETLFEFWYVFLMVLTGI
                     FAVVSSFLAMFQINGEIALWTLVFLPILGIVIWYYQKFSSRIYRNMREKLSQLNTKLN
                     ESISGMRIIQQFRQEARLAKEFEQVNNEYLDTRYAMIKTNSILLSPIINFLYALAIAL
                     VLTLFGIDALHSPVEVGLIYAFTTYVQGFFNPMSQMMDFLSIFTDGMVAGSRILKIMD
                     TEEYAPQQNPGADQMITSGKIEFRNVSFSYDGEHNVLNNISFVANPGETVALVGHTGS
                     GKSSIINVLMRFYEFYEGQILIDDHDIREYPMEELRKKMGLVLQDAFLFYGDIAGNIR
                     MMNPAITDEQIRHAAEFVQANKFIDELPKGYHSKVIEHGASYSSGQRQLITFARTIVT
                     DPKILVLDEATANIDTETEGLIQEGLAKMRQGRTTIAIAHRLSTIRDAELILVLDKGH
                     IVERGNHETLLAQNGLYADMYKLQSSGI"
     gene            46879..47730
                     /locus_tag="IWO89_000241"
     CDS             46879..47730
                     /locus_tag="IWO89_000241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAQ0455029.1"
                     /translation="MLIEKVLNNNVVITRDERNQEMIVMGKGLAFKKKVGDPIFDSCI
                     DKKFQLTNDGLSQQFQELAQAIPLEYLEISCEIIEYTKEALQVQLNDSVYIALTDHLY
                     TAIERHKMGIFVPNVLLYDIKQLFPKEYAIGKKTVKKISDKYHIKLSEDEAGFIALHL
                     VNAQGIQSQQDNTYLFTITIQDILNIVRYYYKITFDEDSVHFFRFVTHLRFFLTRVLD
                     GEKSEVGEVSEELFQVIQKKYKDTFGCVEKIADFLAETFNYQMSTDEKMYLTIHTARL
                     VHENRCK"
     gene            47784..49535
                     /locus_tag="IWO89_000242"
     CDS             47784..49535
                     /locus_tag="IWO89_000242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAQ0455030.1"
                     /translation="MAKPSTNKLEGIEMGKYQELARKIVENVGGKENINGLTHCITRL
                     RFKLKNEEKANDEILKNMDGIVTVMRAGGQYQVVIGNHVPVVFEEVIKAGNLTFDEAV
                     STEKMRPFDMLIDGISGCFQPFLAILAAGGMIRGLTAFLVFLGAFDRGSGTFVMFDNI
                     GDSVFQFMPVIIGLTAARKFKVNEFVGMLLGAALMNPSLSLEALSGAAEAPLTTIFSG
                     TIFEAPIYQTVFGIPWIARNYASSVIPIIFIVLLASQIQKPIKKLVPEMIANFFVPFF
                     TVLITMPLGFLLVGPVFTFATDILMAGFETLLALSPVIYGAIVGFFWQILVMFGLHWA
                     IVPMGLMQFSVNGWQNIMTPVAVVSFGQTAALTALYFKLRNPKDKAIAIPAIVSGIVG
                     ITEPAIYGFTLPRKKIFIFTCVGGALGGAYSGLMNLTSWNQGGLGIFTIPNYIRPDGD
                     LTDVINVLIGIAIAMVVSFTLTFFFWKDEAGETDDIQKKSGKEIVKTPIQGQIAPLNA
                     AKDAAFAQGTLGRGILIYPEKGEVRAPFDGTVMTLFPTKQAIGMVSETGLELLIHVGL
                     DTVQLEGKYFESLVQQG"
     gene            49539..49709
                     /locus_tag="IWO89_000243"
     CDS             49539..49709
                     /locus_tag="IWO89_000243"
                     /inference="COORDINATES: protein motif:HMM:NF012578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0455031.1"
                     /translation="MKQGDLLLRFDKKKIEKEGYSLETPVIVTNYMDYLDILENQGET
                     VGPDDALITALT"
     gene            complement(49750..50652)
                     /locus_tag="IWO89_000244"
     CDS             complement(49750..50652)
                     /locus_tag="IWO89_000244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HAQ0455032.1"
                     /translation="MLLREKIKEISFSPSENEVIQFLLSDSPALGEMTIQEIAERCYV
                     HPSTLIRVAKKMGFDGWVSFRNAFLAETAYLEGNFQDVDANLPFSTTDGIMTIANKMA
                     SLEQMTIQDTLSLIHHDQLQKAKQLMLQAKKIVIFASNSNTLISQDFMLKMKRIKMDV
                     TIVTTFGESFYEAYNCTSDMCGILISYTGENNVILRTAEILKQNHVPILALTSIGDST
                     LSSMSTAALRLTTRERLYSKIGNFTINTSICYLLDVLYSCVFAENYQQNLVHLISVGQ
                     KFDKRPISSAIMKEPFNFENKEVD"
     gene            50882..52285
                     /locus_tag="IWO89_000245"
     CDS             50882..52285
                     /locus_tag="IWO89_000245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HAQ0455033.1"
                     /translation="MAKGIKIATIGGGSSYTPELMEGFIKRYEELPIREIWLVDVEEG
                     KEKLEIVSQMSQRMWDASPYEVKIYATLDREEALKDADFVTTQFRVGQLDARIKDERI
                     PSYYGMIGQETNGAGGIFKAFRTVPVVLAIVEDMKRLCPNAWLINFANPSGMVTEAVI
                     RYGKWDKVIGLCNVPVMATMTEPAMLGKKKEELIYKFAGLNHFHWHKVADNKGNDRTA
                     DLIDRLYDEDNGLPKNIFDIPFFKEQLKQMNMIPCGYHRYYYREEEMLEHALEEYQTI
                     GTRAQQVKETEAELFELYKDPELDHKPEQLQKRGGAYYSDAACETIASIYGNKETQIV
                     VSTKNDGAVPDLPADCVVEVTAYVGAQGARNVAFGSLPAAERGWLQVMKNMELLTIEA
                     AVTGDYGTALQAFTINPLIRSGKTAKRIMDELFIAHQQYLPQFKETISRLKAEGIEVQ
                     DELARELSSNTHVMEEV"
     gene            52285..53718
                     /gene="ascB"
                     /locus_tag="IWO89_000246"
     CDS             52285..53718
                     /gene="ascB"
                     /locus_tag="IWO89_000246"
                     /EC_number="3.2.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HAQ0455034.1"
                     /translation="MGFRKDFLWGGATAANQCEGGYDEGGRGLANVDVVPIGEDRFPV
                     ITGKKKMFEFDKDHFYPAKIAIDMYHHYKEDIALFAEMGFKTYRMSIAWSRIFPMGDE
                     NEPNESGLKFYEDVFKECQKYGIEPLVTITHFDCPMHLIKEYGAWRSRELVGFYENLC
                     RVIFNRYKGLVKYWLTFNEINMILHAPFMGAGLYFEEGENEEQIKYQAAHHELVASAI
                     ATKIAHEVDPENKVGCMLAAGAYYPYTCNPEDVWASRKSDRENYFFIDVQSRGEYPAY
                     ALKEMERNGIQIKMEPGDAALLKEHTVDFISFSYYSSRVSTTDNELLEQTAGNIFASV
                     KNPYLEASEWGWQIDPLGLRITMNDIYDRYQKPLFIVENGLGAVDTPDENGYVEDDYR
                     IDYLAAHIQAMKDTVELDGVDLLGYTTWGCIDLVSAGTGEMKKRYGFIYVDRDNEGNG
                     TLARSKKKSFNWYKKVIDSNGENLSND"
     gene            53863..54789
                     /gene="trxB"
                     /locus_tag="IWO89_000247"
     CDS             53863..54789
                     /gene="trxB"
                     /locus_tag="IWO89_000247"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HAQ0455035.1"
                     /translation="MYDVIVIGAGPAGMTAALYASRSNLSVLMIEQGAPGGQMNNTAE
                     IENYPGFDSIMGPDLAYKMYEGVSRFGTENAYGIVQDIKDHGSHKEVICEDKTYEGKT
                     VIIATGCIHRKLGVPGEEEFAGRGVSYCAVCDGAFFRNKRLVVIGGGDSAVEEAIYLT
                     QFASEVVIVHRRDELRAQKIIQDRAFANEKISFMWDTVVDEIVGNEMVVTGVKARNVK
                     TGNEFDLAADGVFIYVGLDPLTAPFREAGLTNEAGWIPTDNEMKTNIPGVFAIGDVRE
                     KDLRQITTAVGDGGIAGQQVYKYLESLEETTA"
     gene            55003..56730
                     /locus_tag="IWO89_000248"
     CDS             55003..56730
                     /locus_tag="IWO89_000248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-sugar mutase"
                     /protein_id="HAQ0455036.1"
                     /translation="MSWEQVYEQWVHEENLEENLKKQLIDLGEDPEKLEDAFYAPLEF
                     GTAGMRGILGPGINRMNIYTVRQATEGLARFMDTQGEETKRRGVAIAYDSRHQSPEFA
                     MEAAKTLAHHNIPSYVFESLRPTPELSFAVRHLKTFAGIMVTASHNPAAYNGYKVYGE
                     DGGQMPPADADALTKYVREVSNPLKVEVLSDDEAEHSGLITIIGEEVDAAYLEEIKAV
                     TIDHELVETMGKDLKLVYTPLHGTGKMLGERALKQAGFEQFVLVPEQAVADPDFTTVK
                     SPNPEEHSAFEYAMRLGEKEGADLLIATDPDADRLGAAVRLPNGSYEVLTGNQIGALF
                     AQYILEAHQSRGTLPENAAVLKSIVSSELPTAIAEHYGATMFNVLTGFKFIAEKIQQF
                     EEDHSHTFMFGFEESYGYLVKPFVRDKDAIQALLLLAEVAAYYKKQGKTLYDGLQTIF
                     ETYGYYAEKTISVTMSGQEGSAKISALMEKFREEAPTEFAGAKVIQTEDFKELTRTDR
                     EGNVEKLTTPPSDVLKYTLEENGWIAIRPSGTEPKIKFYIGVKGNSSEDAAEKVAAYE
                     AIIKKMTEE"
     gene            56803..57096
                     /locus_tag="IWO89_000249"
     CDS             56803..57096
                     /locus_tag="IWO89_000249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HAQ0455037.1"
                     /translation="MPDTQKTKVEKVSQLFKMLSDPTRLKILLYLKDGEQNVTAITQA
                     VEMEQSAVSHQLRLLRENHVVKSHREGKAILYSLDDSHVLDILNQTLKHVEHQ"
     gene            complement(57190..58050)
                     /locus_tag="IWO89_000250"
     CDS             complement(57190..58050)
                     /locus_tag="IWO89_000250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kinase/pyrophosphorylase"
                     /protein_id="HAQ0455038.1"
                     /translation="MSSDSTEIVTFFVISDSAGETATKLAQATMAQYPTVEFNLFRRT
                     FVTDEKTLMQALQDALKEKALVLHTLINQELIEMTRSFCEEHQLFSFDVMTPPISEIE
                     RLTGIKPIRQPGALHLLNENYFKRIKAMEFAVKYDDGKDPRGFLEADVVLLGVSRTSK
                     TPLSLFLANKNLKVANLPLIPQAHIPKQLWEIDPKKIVGLTNNPDILNNIRKERMRSY
                     GLNPDTAYSDIEKIRAELDFANDLYEKLGCVVINVASLSIEETASLILNALDLEDHSY
                     YGTETTEETD"
     gene            58142..58570
                     /locus_tag="IWO89_000251"
     CDS             58142..58570
                     /locus_tag="IWO89_000251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor"
                     /protein_id="HAQ0455039.1"
                     /translation="MKKQTLIEQSLETLKNNGFKYTKKREALLTYLVKRNRYVSAKEV
                     FDYMNQEFKGVSYDTIYRNLHDFAEIDLLEETDLNGEMKFRFHCCQGEIGHHHHFICT
                     VCGTTKEIHMCPMNYFEEQLGGCTIEGHRFEIFGRCENCQ"
     gene            58732..58908
                     /locus_tag="IWO89_000252"
     CDS             58732..58908
                     /locus_tag="IWO89_000252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010754017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S21"
                     /protein_id="HAQ0455040.1"
                     /translation="MSKTVVRKNESLDDALRRFKRSVSKAGTLQESRKREFYEKPSVR
                     RKKKSEAARKRKKF"
     gene            58935..59381
                     /locus_tag="IWO89_000253"
     CDS             58935..59381
                     /locus_tag="IWO89_000253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GatB/YqeY domain-containing protein"
                     /protein_id="HAQ0455041.1"
                     /translation="MSLLSRLNDDMKTAMKAKDKESLQVIRMIKSSIQNEQIKEGHDL
                     TEEEELTVLSREMKQRRDSLHEFEEAGRDDLAEKVKSEIVIVEKYMPEQLSDEEIRQL
                     VQEAITQTGASSMKEFGKVMGAIMPKVKGKADGNQVNAIVKELLQS"
     gene            complement(59537..60916)
                     /gene="brnQ"
                     /locus_tag="IWO89_000254"
     CDS             complement(59537..60916)
                     /gene="brnQ"
                     /locus_tag="IWO89_000254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transport system II
                     carrier protein"
                     /protein_id="HAQ0455042.1"
                     /translation="MEETKTYSWKQLLMVSSLIFGMFFGSGNLIFPVHLGQMAGANWV
                     SAGVGFAISGALFPLLAILAVVVTKSDGLYDLAKPVGKWYAALFLILVHLTIGPFFGT
                     PRTAATAFEMAAKPFLPEKYNSLGMFLFSALFFLLAYFLTVHPTRLVKYVGKYLNSLF
                     LALLAIIFVVSLFHPMGNLNQSAADTYQTNALTNGVLEGYNTVDAVALLALSVTFVHA
                     VRDLGFKDKKVTELTAKAGSLSVALEVMIYFGLVLLGAMSLNKMSLSENGGTALSQIT
                     NFYLGNFGASFLGVMVTLGVFTTAMGLVVSFAQDFHKLFPKVSYMTWLRLTTFVSFVV
                     ANAGLDNIIQWSLPVLMLLYPLSLALILLSLTAKFFQKTPFVYQVTMLFAAVPAVLDM
                     LANSPALVSQQRVVASMLEFYHHHVPFAALGLGWMVPTLLGYAGSLLFYYAYRLSGYK
                     QEANELPEE"
     gene            61622..62593
                     /locus_tag="IWO89_000255"
     CDS             61622..62593
                     /locus_tag="IWO89_000255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HAQ0455043.1"
                     /translation="MTEESSSLEIRLTAADDVSMLLGAHDKHIKLIEETTETTIHTRG
                     EIIQIIGEQSQISLAASVIRTLQELIKRGIHVSTPDVVTALKMGQKGTLDYFVEMYEE
                     EIVKDRNGKPIRVKNSGQKKYVESVAKHDIVFGVGPAGTGKTFLAVVLAIAALKKGEV
                     QKIILTRPAVEAGENLGFLPGDLKEKVDPYLRPVYDALYQIFGLDHTNRLMERGVIEI
                     APLAYMRGRTLDDAFVILDEAQNTTVAQMKMFLTRLGYQSKMIVNGDTSQIDLPRGTT
                     SGLVHAERTLKQIKKIDFVNFEASDVVRHPVVAEIIKAYEKADLHQE"
     gene            62617..64809
                     /locus_tag="IWO89_000256"
     CDS             62617..64809
                     /locus_tag="IWO89_000256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDIG domain-containing protein"
                     /protein_id="HAQ0455044.1"
                     /translation="MLNKTLQKLQKKLGKAALPFLLFLFFIIVCGVTFSSVKQKANDF
                     REGQVAEESIRANKTIENTEETEQRRKLAAEAVTPEYTYQKDLADDQNNRIKQLFELI
                     DKTNDTINKSYNDKVDKAKEDENIPKPTTEERIATLKKNFENVNAENVAFYQKLPNNF
                     YSTIFEMTETEIHTVRDESLKLIDEQMSKQVRESELEAFKQEAEDQIQYLNVTTEQQQ
                     MIRYLVDQGIVVNDVLNEKKTEELKQSAREAVQPVMIFQGEIIVREGNQIDASAMKKL
                     ELLGLTNQTTSIFPLIAMVLAVLLQIGVLIYNSLQFQNAGERTKYVLFYVTAMSVSVV
                     LMKFFQLFQTEQAAYIPLFYPAAFAPLVLSFFLNRRAGIMAGIFQAVSALFIFYDSIG
                     TNFLTVILMSYVFSGLMATIVRRKRISEQGLSAAMWVIIFPVMMDVILIIYQGMSFGD
                     ATTWLTLVCGFTGALFSFLLTIGLHPYIELMVNDDSVIVLNELSNPNHPLLKQLLEEA
                     PGTYHHSMMVANLSANAVAEIGGRSLLTRVACYYHDIGKIKHASFFVENLPSGAENPH
                     NFLLPEDSKHIIFGHVTDGAKILEDYNMPQMVIDICRQHHGTTLMRYFYVKAKERNPE
                     VTEEQFRYPGPKPQSREAGIVNIADSCEAAVRAMDHPTSEKIESFVHNLIEERISDGQ
                     LDDSGLTLKEIRKVEKSLISGLSSTFHSRIKYPKMKSEVEKIKEEQEE"
     gene            64826..65302
                     /gene="ybeY"
                     /locus_tag="IWO89_000257"
     CDS             64826..65302
                     /gene="ybeY"
                     /locus_tag="IWO89_000257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HAQ0455045.1"
                     /translation="MDITFMDETNQVSEEKMKEIDDLLQFAASYLELPEETEMSVTFM
                     DNAAIQVINRDYRGKDAPTDVISFALEEEGEDEIPVIFEDGENPLPRVLGDIMISTER
                     AAEQAEAYGHSYDRELGFLAVHGFLHINGYDHMTPEDEKEMFGLQKEILDAYGLKR"
     gene            65280..65681
                     /locus_tag="IWO89_000258"
     CDS             65280..65681
                     /locus_tag="IWO89_000258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family protein"
                     /protein_id="HAQ0455046.1"
                     /translation="MPMDLKDKNVEKNKHFLMSVEFAVQGVKTVFEEERNMRKHVAFG
                     ILALFMGVIFQLDRIEWLWLLLAVFLVWIVEIINTVFENVVDMFTDFHFHPIGKKIKD
                     MAAGAVLVTACFSVIVGLILFVPKIYHLLFN"
     gene            65696..66595
                     /locus_tag="IWO89_000259"
     CDS             65696..66595
                     /locus_tag="IWO89_000259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase Era"
                     /protein_id="HAQ0455047.1"
                     /translation="MPEHKSGFVAIVGRPNVGKSTLLNRIVGQKIAIMSDKAQTTRNK
                     IQGVYTTPEAQLIFIDTPGIHKPKHRLGDFMVEAAYSALKEVDAVLFMISADQKRGRG
                     DDFIIERLKNVHSPVYLIINKIDKIHPDELLGIIEDYSTQMPFAQVIPISATEGNNVE
                     RLMDVLVSEMPEGPQYFPDDQVTDHPEYFIVSELIREKVLFLTRDEVPHSVAVVVDSM
                     KRNENDKIHIQATIIVERDSQKGIIIGKGGKMLKQVGTKARKDIENLLGDKVFLELWV
                     KVQKDWRDKKVYLQDFGYRKDEY"
     gene            66738..67550
                     /gene="recO"
                     /locus_tag="IWO89_000260"
     CDS             66738..67550
                     /gene="recO"
                     /locus_tag="IWO89_000260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HAQ0455048.1"
                     /translation="MSQLAESKGLILSSRNYKEKDKLVKIFTESSGKMMFYIKGIHRQ
                     NQPLAPALLPFTEAVYIGNFRSEGLSFLSSVKDVHAFRHIQEDIFLSGYGTYLLNLVD
                     AAIEDHQYDPHLFQFTQQALQLMDQGDDAEIITNIFEVQILQRFGIAPIWTHCVVCGE
                     TKGKFDYSSKYGGVICERHWSMDDHRYHADPRAVYFVRMFSAISYDKISGIHLKEETK
                     QAIRELIDQLYDEYVGIHLKSKKFIDQMKSWAHVLKTSEKDMSEKDLPKKEE"
     gene            67936..68853
                     /gene="glyQ"
                     /locus_tag="IWO89_000261"
     CDS             67936..68853
                     /gene="glyQ"
                     /locus_tag="IWO89_000261"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit alpha"
                     /protein_id="HAQ0455049.1"
                     /translation="MSKKLTVQDMILTLQKFWSSNGCMLMQAYDTEKGAGTMSPYTFL
                     RAIGPEPWNAAYVEPSRRPADGRYGENPNRLYQHHQFQVVMKPSPENIQELYLESLRL
                     LGIDPLEHDIRFVEDNWENPSMGCAGVGWEVWLDGMEITQFTYFQQVGGLPCKPVTSE
                     ITYGLERLASYIQEVESVYDLEWADGVKYGEIFKQPEYEHSKYSFEVSDQDLLLSNFD
                     RFEKEAKRCIDENLVHPAYDYILKCSHTFNLLDARGAVSVTERAGYLSRIRNMARAVA
                     KIFVAEREKLGFPLLNKNTANPENVKEAE"
     gene            68855..70930
                     /locus_tag="IWO89_000262"
     CDS             68855..70930
                     /locus_tag="IWO89_000262"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010728817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit beta"
                     /protein_id="HAQ0455050.1"
                     /translation="MAKDLLLEIGLEEMPAHVVTPSMKQLEEKTAKFLDENQLTYDTI
                     ETFSTPRRLAIKVTNIPERQADSEEEVKGPAKKIALDADGNWTKAAEGFVRGQGLTTD
                     AITFRELKGVEYVYVTKYTHGKESKEVLTGLADVIKSLTFPVTMHWAEYDFEYIRPIH
                     WIVALLDDEVIPFEILDVKTGRASRGHRFLGEDVTFAHAKEYEEKLNEQFVIASPQKR
                     QEMIVTQANELAEKNHWVIDLDEDLLEEVNNLVEYPTVFSGDFEEKYLSVPEEVLVTS
                     MKEHQRYFEVRNDQGMLLPHFISARNGDRVKLENVVRGNQKVLTARLEDAEFFYNEDK
                     KASIDQYVEKLKTVTFHEKIGSIYEKMQRVQVIARIIGEQVGLSEEELMDLQRAAEIY
                     KFDLVTNMVGEFPELQGIMGEKYALLKGEKPAVATAIREHYMPISSEGELPQSTVGSV
                     LAIADKLDSIFSFFAVGMIPSGSNDPYALRRQAYGVVRIIESKKWAFPLSVLQETISD
                     VISKDTDRFGIGLSEGQQQVIDFIKGRLRQLLTTKNIRHDVIDAVLNAEQKDLTKVFA
                     AAQLFKQHLADEDFKPSMEALTRVVNLAKKAQLEKQSEVDPELFENDAEKELHKAVEA
                     MKASFADQTIHENYDQLVALRPLIENYFDQTMVMADEPAVRKNRLTQLSQIAAMALSL
                     ASLDQIITK"
     gene            71315..72094
                     /locus_tag="IWO89_000263"
     CDS             71315..72094
                     /locus_tag="IWO89_000263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0455051.1"
                     /translation="MEKPFVKLLATVAVGTGAIVICLFGYHFNNQRQHHQRINYAESA
                     ITNQKDTVTSLSKEVDKLYSTKEKIFLNPEITEETVSNLSHKLSSIKLSADDYDIKES
                     ELPKEAAAIQEEKKAVLTQLEDAESKLKIQTAVNDLFTKNVSNWQQAVDDVVIKEKVT
                     SADVARVRENMSFFKDSPWKTVVMQYLGFADTQIAQVTQLDQLFDTMLKDGQVTATAT
                     YDQYLTALSLIEQIRNEKIRAAYASKAETVAQQMGYSNTSY"
     gene            72135..73079
                     /locus_tag="IWO89_000264"
     CDS             72135..73079
                     /locus_tag="IWO89_000264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HAQ0455052.1"
                     /translation="MAKRRRRSRGKKIFLIILAILVVLVLAIVGVGAKLYLDLSHSIE
                     KTYESVERTQGERTKPVDLDKQESFSVLLMGIDTGDLGRVDQGRSDTMMVATVSPEDK
                     QTTIVSIPRDTYVDIVGHDTKDKINHAYAFGGAAMAMDTVQKYVDIPIDHYVSINMKG
                     LKELVDAVGGIEVNNDLTFSQDGYDFTIGKITLNGDQALSYSRMRYEDPNGDYGRQER
                     QRKIVEGVAKKVLSLNGVSNYQSVLNALESNMKTDMSFDEMKKIALDYRDAFGTIKQD
                     QLQGEGFMQDNISYQKVSDQELDRVKKELKDQMNLENK"
     gene            73095..73874
                     /locus_tag="IWO89_000265"
     CDS             73095..73874
                     /locus_tag="IWO89_000265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine protein kinase"
                     /protein_id="HAQ0455053.1"
                     /translation="MEETISLKEIFETLRKHVITILISMFAGLAISGIVTFFILTPKY
                     SSQAQLIVTLPQSDTANVNDVNTNLQMINTYKDMIVSDLVLNEVKDRLETEDNVKMTA
                     GQIKDAISVNQSQNSQMFSIQATSTNAVTAQQIANTTSQVFQENAKDVMNVDKISIIS
                     GAVANTTPVSPNNKLNLVIGLVLGMMVGVGLAFLFELLDRTVKDEKYVTDTLGFPILG
                     TVPEMSAKELNAGIRKKGPAPTQPKATNDKTAPSRRSRSRV"
     gene            73887..74585
                     /locus_tag="IWO89_000266"
     CDS             73887..74585
                     /locus_tag="IWO89_000266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CpsD/CapB family tyrosine-protein kinase"
                     /protein_id="HAQ0455054.1"
                     /translation="MARTQKQKAAQTHAVSLVTLTNPSSPIAEQYRTIRTNIQFASAA
                     GQQIKTIVVTSSGPGEGKSTTAANIAVVFAKSGQRVLLVDADLRKPVIYKTFKLNNAS
                     GLSTVLSSSGSVADEIQRTPVENLSILPSGPKPPNPSELLSSPRMDQILAEARQLFDV
                     VIFDMPPVVAVTDAQIMSSKTDGTLLVVRENMSRKESLTKAKELLDMVQARVLGVVYN
                     GAEHSKDAGYYYGN"
     gene            74613..75377
                     /locus_tag="IWO89_000267"
     CDS             74613..75377
                     /locus_tag="IWO89_000267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine protein phosphatase"
                     /protein_id="HAQ0455055.1"
                     /translation="MIDLHCHILPGIDDGAENIDASIAMAEKAIQQGITHILCTPHHN
                     NGKYSNEKSQVISLVASLQAELEKRQLPLTLLEGQEVRITGTLIEDIDRDEILFTDLD
                     DTYLLIEFPTLEVPLYAEQLFFELLQLGKTPVIVHPERNAHFRKDPNHMLPFLDMGCL
                     AQLTAPSYVGVFGKDIQKTAKMMVKHNLVQMAASDAHGVSKRTFYLKECYEQIAKDFG
                     KEKVVQMKQVAKDLINGDEISYPVYEEVKKKKFGLF"
     gene            75422..76111
                     /locus_tag="IWO89_000268"
     CDS             75422..76111
                     /locus_tag="IWO89_000268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HAQ0455056.1"
                     /translation="MESEDLLSRISEVHPNQVWLDKEKVEKRYIYLFFKRMIDIVGSI
                     IGLILASPLMFYVAYRIRKEEPGSPIFFCHTRVGKNGKTFKMYKFRSMCLDAEEKLEE
                     LLDQNEIEGAMFKMKEDPRVTKIGKIIRAKSIDELPQLWNVLKGDMSLIGPRPPLERE
                     VAEYTRYDLQRLYVKPGCSGLWQVSDRNNVSFHKMVELDLEYISHRSLMYDLRLILKT
                     FYVIVKPNGAY"
     gene            76454..76627
                     /locus_tag="IWO89_000269"
     CDS             76454..76627
                     /locus_tag="IWO89_000269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1972 domain-containing protein"
                     /protein_id="HAQ0455057.1"
                     /translation="MNRYVHGTVQKKNRKINGKLLVNPNGYEWLRAKGSYPVPKYCNY
                     SETLMEWYQLIIT"
     gene            77973..79223
                     /locus_tag="IWO89_000270"
     CDS             77973..79223
                     /locus_tag="IWO89_000270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide sugar dehydrogenase"
                     /protein_id="HAQ0455058.1"
                     /translation="MKKQYNIAVAGTGYVGLSIATLLAQHHKVTAVDIIPEKVELINN
                     RKSPIQDDYIEKYLAEKELNLTATLDAKAAYQSADFVVIAAPTNYNSRTQHFDTSAVE
                     AVIRLVMEVNPDAIMVIKSTIPVGYTASVRVKTGSKNIIFSPEFLRESKALYDNLYPS
                     RIIVGTDMDDERLVEDAHTFATLLQEGAIKENVDTLFMGFTEAEAVKLFANTYLALRV
                     SYFNELDTYAEMKGLNTQQIINGVCLDPRIGTHYNNPSFGYGGYCLPKDTKQLLANYA
                     DVPENLIEAIVESNRTRKDFIADRVLQMAGYYNYAEANQYDAGKEKPVVIGVYRLTMK
                     SNSDNFRQSSIQGVMKRVKAKGATVVIYEPTLKDGETFFGSKVVNDLEQFKAMCDSVI
                     ANRYDACLDDIKEKVYTRDIFQRD"
     gene            79242..80252
                     /locus_tag="IWO89_000271"
     CDS             79242..80252
                     /locus_tag="IWO89_000271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kinase"
                     /protein_id="HAQ0455059.1"
                     /translation="MIITKTPFRMSFFGGGTDMEDYFKENGGAVLSTTFDKYCYVNVR
                     HLPRFFDYSAELSYSRTERVTSIEDIEHPAIRNAMKMLDMHEIRLTYEADLPARSGLG
                     TSSSFAVGMLNAFYALEGKYADKKKLADEAIYLERVLCNEAGGWQDQIAASYGGFNRI
                     NFNVDGYEVLPVIISPERKKQLNNNLMMFFTGFTRFSSDVQKANNVSGTEDKRVRLKK
                     MYELVDEAEAILTDKNRNLDDFGRQLDVTWRLKKGTGGAISTGSIDELYEKGMAAGAL
                     GGKLLGAGGGGFLVFYVQPEKQDAVRWAMRDLMYIPFEFENGGTRVIHYTPETYIEKQ
                     NG"
     gene            80262..80915
                     /locus_tag="IWO89_000272"
     CDS             80262..80915
                     /locus_tag="IWO89_000272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HAQ0455060.1"
                     /translation="MLAEKLNKHIDLLIKRYPTLETIKQDIIDGYLIMEECYLNGGKL
                     LVAGNGGSAADSEHIAGELMKRFKTPRPVSKEFADKLVAIDPVRGPELAKNLECGLTA
                     IPLVAHEALSTAYINDVDGLGVFAQQLFGFGRSGDVFLGISTSGNSKNIMSATVVARA
                     LGIKVIGLTGAKGGELATVADVCVKAPEVETYMIQEYHLPIYHCWCLMLEDKFFGNE"
     gene            80934..81932
                     /locus_tag="IWO89_000273"
     CDS             80934..81932
                     /locus_tag="IWO89_000273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HAQ0455061.1"
                     /translation="MKKTIMVTGGCGMIGSNLVKRLVKEGCWDVYVADNLWRGKLEYL
                     NDEDGHPVIDLDTHFFNADLTDYEQAKKVIGTTEYVVHLADVVAGIDYVFKNQGELFR
                     INNLINSNVFDCCRKAGKEKIKGVIYVGTVCSFPLTRQNTLNPEPLREEELFPALPES
                     AYGWSKLMGQLEMGYLEKETGIPCCILMLHNVYGTPTDFGERSQVIPALIRKAINNPE
                     EPFNVWGSGEQGRAFIHVNDIVNALVLALDKGWGHGHIQIGPSHCTSIKEIAYKIIEI
                     SGKDIKPFFDTSKPEGDKARCADYSKAKEILDWEPTVSLEEGLRESYNWIEKQIKG"
     gene            82008..82769
                     /locus_tag="IWO89_000274"
     CDS             82008..82769
                     /locus_tag="IWO89_000274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WecB/TagA/CpsF family glycosyltransferase"
                     /protein_id="HAQ0455062.1"
                     /translation="MEKNMYKRKRDFTVCGIVGVCATTKNEFVREVFSMPTSPVSATV
                     DLVGVPAIISAKENPKIAEMYSQATMAAIDGMPIVKIGRRKGFTCERCAAPDIMGMVF
                     EEGVRQGKTHYFYGGKDNEVLNKLRANLERDYPGIKIVGMYSPPFRPLNEEEDSKICE
                     EINNLNPDFLWVGIGAPKQEMWMWEHQKKIHNTVMFGVGAGFNFFAGTLEKAPEWMEK
                     ASLEWLFRLVKEPRRLWRRYILGGIKYIYYTIESK"
     gene            82787..83893
                     /locus_tag="IWO89_000275"
     CDS             82787..83893
                     /locus_tag="IWO89_000275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HAQ0455063.1"
                     /translation="MKILIVVTMTNNFGKKGSYNSQEIGLARGLAELGHEVIVYKSVK
                     SDEEVGTEQLSNTAMIHYIKCRYVGSNGFLSTKNFDKNADVLIQFADLQLSVPHVYSW
                     TVKNGIKYIPYNGITRSQSGNKYIKTIIEILAHRNYKIYKKNGCVAKNDRVSEKLVQL
                     GVNKVKLAPVCVDFEQLHKNYEAIDFTTLREKWNIPRNVRVILYVGRFEKDKRPLDMI
                     SLFNKLDEHNYLLMVGTGALLEQTKQQINLLGLQNRVKIIERIPNKDIWELYCLSDVF
                     VNLNYTEIWGMVLLEAMYYRLGIIAVNAPGPQNIILDNRYGMLAKSDDEILTFLKDGR
                     FTNDIIDAAYQRVTTEFRWSKSGEIMLSMLEGDK"
     gene            84315..84770
                     /locus_tag="IWO89_000276"
     CDS             84315..84770
                     /locus_tag="IWO89_000276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine acetyltransferase"
                     /protein_id="HAQ0455064.1"
                     /translation="MNAYKLYKVERWFYLHKMEFFAKIVKALIYVLHNSSISYQTEIG
                     DNCKFLYGGIGCVIGKDTKIGNHVIIGTNVLTGGRSNKPGMPTIGNNVYIATGAKILG
                     NITIGDNVIVGANAVVINDVEPNCSVGGVPARVLKKDIDVNDYCSLNRY"
     gene            84784..85557
                     /locus_tag="IWO89_000277"
     CDS             84784..85557
                     /locus_tag="IWO89_000277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002358036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HAQ0455065.1"
                     /translation="MLYFVIPLMSAKAANNWEMVSTLFNRTLWSCYNQTDPDFKIIVA
                     CHEIPKLTHEYDDRVEFIQVSEKDAPIPTNQDEKMIDKGYKTHALAMRLRELGGGFAM
                     MVDADDLVSSHLAEYVNSHPQENGFYVKTGYVYFVGDDYMKVLPKFSSGSACIVKYAV
                     EDLPEAYPEVMTANCDDNPWIIRKRHGGVVPACEEQGRPLKPLPFKAAVYVLGTGDNH
                     SLYGKTTKYQTKMREILEMFIAKRKIKGKLKAEFSVDWL"
     gene            85544..86674
                     /locus_tag="IWO89_000278"
     CDS             85544..86674
                     /locus_tag="IWO89_000278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HAQ0455066.1"
                     /translation="MIGYKVSIMKVLMCESQMPENWYSFDLSKELNQYVDLTLLCKRD
                     VPPLDPKIKSKHILYHKSTNKVFSALVFLKSLFLIWKEIRFGDYDIVHLQLMKSLKYE
                     GGLYVLCKRHYKKLIMTVHNVLPHEVREKDRQEHFNIYSNCDGFIVHNRVTEQHLYAE
                     FPELNAKSYVCPHAIYGDVKHISRAYDKDKFEFILFGQIRPYKGIDVLLNAITELPMD
                     VRKKVHFTIAGQQHPKQDSTDYKKMIEDLKVSDCVLYKPERISDEEMDSMFRNADAAI
                     FPYKEIYGSGALLMAYTYCLPTIVSNVPAFMEETEEGKIGLLFENLNSKSLADTIVKY
                     VNLSPAEKSKIEDDIYELVNRKYTWAASAKLTYEAYKQILNE"
     gene            86705..87940
                     /locus_tag="IWO89_000279"
     CDS             86705..87940
                     /locus_tag="IWO89_000279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0455067.1"
                     /translation="MQISKWKIIQFLMVFLMIISSSILKPLGGWLGETMNVRLVTVIL
                     AIVCLLIASLPAFRKNRYLNFCTLLICVVIIAAYIYSDKTIWVKSRESMSLYYVLLAY
                     PLFCTLINQAWNLDSMLNTICGVTFLSYILRTSISLIQKFSGVLLYENIYRECAPENW
                     YRGGFLRINPPCFTIIIIPIALYMIERQVVARRKVKYYLLIASALAYSAVIHGSRSVL
                     VYQIIVLGCYILFKKGSSKRKIAMWVLAGLLVVIFINSTMFSTFVETFTNSTGEYAGS
                     AESRFASVWFFIPKFLQSPLFGTGILTGEEFTFILPGGVRGHLSDIGFFYTITQYGVG
                     ILIFDILFIIKGFKTALLASKVGMTGYQYLAFGITLNVLLTGINIDWFYPIFTPAIPV
                     CLAVIEYIYQECRSVANIE"
     gene            87965..89074
                     /locus_tag="IWO89_000280"
     CDS             87965..89074
                     /locus_tag="IWO89_000280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0455068.1"
                     /translation="MNKKEILYNILKKYENLPYVFRNMFGHNISKLVSWKLTADCATV
                     EENSEVYAAFQLRSKDMSDVPVMGLVNCCKDVAIVMQGPLCLKDDFSYNTLAYYKKCY
                     PNALIILSTWKDEDIKALKRIEDLGVIVICSEKPEKPGHLNINYQVGNTLPGIVRAKQ
                     LGAKYVCKTRTDQRIYHPNAMAFFCSLLEQYPVNNEDDWKLIQNQRLLLLSMPYGDMF
                     FPYCLSDFLYFGDVDDMINLFSIPSDVREKGAVSRGVSRREISENNLAPEVQLLRSYI
                     SRMGGNEECSIRAYWEFVKNHVITINKNQIDLYWHKYVGRYSNNTIYGTYYIDDSQDA
                     LHCYNFDFINWLNLYTGKYEYKAEYEKYMDFVWEE"
     gene            89116..90450
                     /gene="gmhB"
                     /locus_tag="IWO89_000281"
     CDS             89116..90450
                     /gene="gmhB"
                     /locus_tag="IWO89_000281"
                     /EC_number="3.1.3.82"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-glycero-beta-D-manno-heptose 1,7-bisphosphate
                     7-phosphatase"
                     /protein_id="HAQ0455069.1"
                     /translation="MKTVIMAGGRGSRISELFPDIPKPMIPIDGIPVLERELCSLRDQ
                     GFNDIILTVSYLHEKIEEHFGDGSKLGIKIKYFIEKTPLGNAGALYFLLDELGDEPFL
                     LLNADAVFDVDFRQMIRFHVENNADVTLFTHPNSHPYDSGLIISDDNKKVLQWLAKED
                     QRPEYYKNRVNAGLHVINPKVLRENPLMEIVNKSIVEGGGLPYKIDLDRMILKPLANT
                     GRMYCYDSPEYVKDMGTPDRYRAVIKDFQKGIIVGKNLSNPQKAIFLDRDGTINKYVG
                     FLRNAEEFELIDGVSEAIKIINSLGFLCIVITNQPVIARGEVTVDELNSIHNKLETML
                     GHAGGFLNGIYYCPHHPHKGYAGEVIELKIDCNCRKPKPGLLLEASKDFNINLSQSFM
                     VGDSESDVIAGKNAGCKTVLITEDITLPANYGQDYSVCSLLEFVKNHLCTMQ"
     gene            90485..92122
                     /locus_tag="IWO89_000282"
     CDS             90485..92122
                     /locus_tag="IWO89_000282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsular biosynthesis protein"
                     /protein_id="HAQ0455070.1"
                     /translation="MKVLIPAAKVVEKELQSIGMLPAIIYPINKKIVFDYLYNQYNAI
                     CDSLDIICYEKADKVHRRLKNYHDSRIHICDLPELGDLGQTVYFGLSDEDEPVLINFA
                     DTIVMESMSDIEMDTFYYSEDFVSSTWTYFKINEGIITDIHDKEEAYVSDKREKLFIG
                     VFMFSSSKLLKSCLREAVESRKQGGINSFYRALQLYSERCPLKAVRTEKWFDIGHIDK
                     YYNTKLEVKAREFNHISIDKDRGILKKTSDDKDKFIGEIKWYLKLPADIEYVRPRIFD
                     YSTSYVAPYVDMEYYAYHTVHELYLYGDLKYNQWHDLFSRIKFICSDFKRYTLKDEKI
                     RESLEDMYLRKTMQRFQKLSEDKRFSAFFEKQIQINGRKFISLTGVIELLKNLIPSML
                     YDVNEFCIIHGDLCFANMMVDSNCSFVKVIDPRGKFGKYDIYGDSRYELAKLFHSLDG
                     KYDYIIKDLFTVEYDTECAVIDYTINTRVRDYDLFELFKSVFSDEIKDDFDKIVLIEA
                     LLFLSMIPLHGESFDHQIVMLATGMELLARVSNKVIL"
     gene            92224..92619
                     /locus_tag="IWO89_000283"
     CDS             92224..92619
                     /locus_tag="IWO89_000283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsular biosynthesis protein"
                     /protein_id="HAQ0455071.1"
                     /translation="MYSEYSFVFDIDGTLCPIKKKDERYEDLVPYENMVEKLRFYKEN
                     GAKITLFTSRNVNSYKGNLGMINANTAKVLLAWLDKWDIPYDEILYGKPWPGHKGFYV
                     DDRTVRPDEFLRCSVDELNEICNSSKKRF"
     gene            92637..93368
                     /locus_tag="IWO89_000284"
     CDS             92637..93368
                     /locus_tag="IWO89_000284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0455072.1"
                     /translation="MEKKMTVIITMAGLGSRFRKIGYAVPKYMINAKGRTLFDWAMDS
                     LVGYNKNVDKYIFVVRQEDNASAFIKEHCAYFGIENVEVIEIDYMTDGQATTCMLAIP
                     YCNQDNEIMVYNIDTYVESNEMKYEDICGDGHIPCFHADGDHWSFAKLNDRNEVIEVR
                     EKVRISDNCTLGAYYFSSAKLYKALYEEYYADSSNMEKNEKYIAPLYNFMIKKGLKVT
                     ISIVDAKKVHVLGTPEELNVFLNQE"
     gene            93392..94843
                     /locus_tag="IWO89_000285"
     CDS             93392..94843
                     /locus_tag="IWO89_000285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HAQ0455073.1"
                     /translation="MTSNNLIVKTLSRYKNTPLPVKAALWFMVCGFLQRGVSMITTPI
                     FTRVLSTEQYGLYSTYLSWESLLLMITSLSLFKAMMNLYVKYDNQEHILSTVSGLTLV
                     LTIFWFVIAIFIKDILAEILGLTPVLVVCMFSYFIFQAEIQCWSLHKRYIYDYKTLLV
                     ATLAVTVGSSVVGCLAVLFISPTAEARALMNVLVTGVVGVAVYISVFRRCKDFKDNEV
                     WNFAVVFCVSLMPHYLSEFILNGSDKIMINYLCGSSDVAIYSVAYSIGALINLVTNSV
                     NSTFAPYQYQKIKSGEYDLLSKRANEVLGLVAAMLFALMLFSKEIVLIFGGQKYIDSV
                     NLIVPICIGIYFNYMFQLFARVQEYFEKKLTVVIPSVLCAIMNIVLNYVFIKLYGYQA
                     VAYTTVFSYMFFCVIHYIFYRKVCKELLDGKNIYDVRGILLISAITIAGGILISIINK
                     FDFIKYLIIAIACVAAIIFRKRLLLLVRRIMSK"
     gene            94869..95480
                     /locus_tag="IWO89_000286"
     CDS             94869..95480
                     /locus_tag="IWO89_000286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HAQ0455074.1"
                     /translation="MIDIISAITVNVLTALYQPFLFAVLTAYLFMYWYLFIVDNSRDP
                     KNLKDSVLIWLRYFKASSKFRRLFFLAFYFTLILFKTLLNRNMWMNPLSNVIGQWSLY
                     IIDNATGESRLTTECVENTMLFIPFCMLLLWWRDIKCDIIRTIYVGIKYVFLFSLSIE
                     FTQLFFRLGTFQLSDLFYNTLGGLIGALLYWLFYRLNKYIDLK"
     gene            95662..96177
                     /locus_tag="IWO89_000287"
     CDS             95662..96177
                     /locus_tag="IWO89_000287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-keto-6-deoxy-D-glucose epimerase"
                     /protein_id="HAQ0455075.1"
                     /translation="MVVVMVKVFDIIKFSDFRGYTAVALDNETLFSIGIEFHIKQINQ
                     GFSHNKYTMRGLHYQSGEHAQGKIVMCLHGEIMNIALDIRQGSSTYGCVYVNTLSESN
                     GKCAYIPRGYAHGYLTMEDNTLVQWCVDNDFNSNAAKCISYESVDIEWPVSRQCFVIS
                     EKDKNVERLSF"
     gene            complement(96312..>96489)
                     /locus_tag="IWO89_000288"
     CDS             complement(96312..>96489)
                     /locus_tag="IWO89_000288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS982 family transposase"
                     /protein_id="HAQ0455076.1"
                     /translation="KTVETVFSSLEKLGCQNFNSRSVKGLESRFESILLAYSVLLSRA
                     QRRFEGTLRYSLGY"
BASE COUNT        31573 a        16227 c        20689 g        28000 t
ORIGIN      
        1 ttttgtctca gtcccttttt ttgcatattt ttagcattta tttcctaaaa aatgtaaggg
       61 gattcctata tttttaagta tacttgtttt tccttttata cattttatgg tattatcatt
      121 ttattagtta ttataaaaca atcgttagaa aggacgtcat tatgcaagta tacggaattg
      181 atgcggtgct tcgaatcgtg agtcatttgg cgtttatcta cttagccttc tggtctttac
      241 gttcgttacg cattgaaact tttttcagac caatgcataa tacgcaaatt agaatggcaa
      301 tcgtactttt ttcaattttt ttagggtaca ctgcaagtaa ttttttctta gaagttatcg
      361 ctttatgccg aaatcttttc gttagcctgt aaattttaaa aaaaggtact attttaaaag
      421 caaggatgtt ataatagaaa agattttgag taagtgtatg ctcattggaa ttttttggag
      481 ggaacaacat ggaagagatc atcgtaagag gtggcaatca attaaatgga acagtaagaa
      541 ttgaaggagc taaaaatgct gttttgccta ttttagctgc tagtttgtta gcagaagaag
      601 gtattacgac attagacaac gttccgattc tttctgatgt cttcactatg aatcaagtga
      661 ttcgtcactt aaacgtagat gtagattttg atgaacaaaa gaatcaagta acaatcgatg
      721 cttcaagaca attagaaatc gaagcgcctt atgagtatgt tagtcaaatg cgtgcttcaa
      781 tcgttgtgat gggtccgcta cttgccagaa atggacatgc aaaagtagct atgcctggtg
      841 gatgtgcgat tggtaaacga ccaatcgatt tgcatttaaa aggatttcaa gctttaggag
      901 caaaaattat tcaaaaaaac ggctatatcg aagcaattgc ggatgaattg atcggtaaca
      961 cgatttattt agatttccct agtgtaggtg ctactcaaaa tatcatgatg gctgccgtaa
     1021 aagcaaaagg aacaaccatc attgaaaacg tagctcgcga accagaaatc gttgatttag
     1081 caaacatttt gaataaaatg ggcgctcaag tctatggagc tggtactgaa acaatgcgta
     1141 ttgaaggcgt ggatcattta catgcagtca accactcaat cgtacaagat cgtatcgaag
     1201 caggtacctt tatggttgct gctgcaatga cacaaggcaa tgtgctgatt gcagatgcta
     1261 tctctgaaca taaccgccca ttgatttcta aattgatcga aatgggtgca gaaatcattg
     1321 aagaagaagg cggagtccgt gtcatcggac caaaacatat cttacctaca gatgtaaaaa
     1381 cgatgcctca tcctggtttc cctacagata tgcaagctca aatgacagct atccaattag
     1441 ttgcagaagg tacaagtgta gtgactgaaa cagtgtttga aaatcgtttc caacatttag
     1501 aagagatgcg ccgtatgaac gctcatgtca aaatcgacgg aaatgtagca atcatggatg
     1561 gaaaccatga attgcaaggt gcagaagtat atgcaacaga cttgcgggca gcagcagcat
     1621 tagttttagc tggcttgaaa gcaaatggaa tcacacgtgt gcgtaacttg aattatctag
     1681 accgtggata ttataatttc catatcaaac tacagcaatt aggtgcagac gttgaacgtg
     1741 tagacatgga tcaaacttct gctgaaaaaa cagcacaaac gattgcttaa ggaagtggct
     1801 gaatgagtaa ccaacgatat atcttattaa ctttgataaa gattttagtg gtgatcttat
     1861 tgcttatcct tctttttgtg gccggaacga tgatcggata tggtgtcatc ggtggcggca
     1921 atccatttaa agtattccag ccaagtttgt ggattcatat tcgagacttt tttcattgat
     1981 aaataagaat cacagtggtt ctagtcgata tgaagtgagc catgaagcag aacatgacaa
     2041 aagaaaacag aagggaacgt tgacgttagt cgcgatccct ttttttagta aaagaaaaga
     2101 aggaatctaa ttgatgaaga agatattgat ttatttagtt agaggatatc agcgctttat
     2161 ttcgcctgca tttccaccta gctgccgtta ttacccaacc tgttccaatt atatgataaa
     2221 agcaattcaa acccatggtg cactaaaagg gacggtaatg gggactgcac ggatcttacg
     2281 ctgtcatcct tttgtcaaag gcggtatcga ttatgtacca ttgacattcc aattaacgcg
     2341 aaataagaaa gataaaaaag aaccagatta taaaaaggtt gtgaaaaata cgagtagcac
     2401 tgagcgataa agaaaaagag gaaaattatt gtcaaagtaa caactccgca actacttata
     2461 tgggggacaa aaaatgatcg aagaactaaa caagaaagat ttaccatggt ccttgttatt
     2521 agatgcagat cctgataaag aaaaagttca agtatatgtt tccagaggga gtggctggat
     2581 atggaaagaa aaagaaaaaa caataggtgt actgatctat gtggcaaggg aaaaagaata
     2641 cgaaatcgtc aatgtagcag tagccccaac gtatcaagga aaggggattg gcggaaaatt
     2701 acttgagaca gcttttcaga agctttcaca actcgcttcc agtcaaactc agatcatcat
     2761 acgaaccggc agtactagca gtgcggcgtt acatctctat caaaaaatgg gctatgtgga
     2821 gattggtcga gtcaaagact attttatcca tcattactct gagcccattt ttgaaaatgg
     2881 agatcaatta agggatcaag taattttagc aattcactta cctaaaaata gctttaacta
     2941 ataaactttc aattcgagaa aatgctttca aatgctaaat agatacatta caatagcttt
     3001 agcaaacaaa aaggagaata tttttatggg aatattagtt tttgattttg gtggatcggc
     3061 tgtcaaatat ggttgttggg atggcaaaga gattaaaggt aagggacaat ttgcaacgcc
     3121 tgaatcttgg gaagaaatga aagcgcaatt atttcaagtt tataaaaaaa tactcgtttc
     3181 ttttgaagtc gaaggaattg gaatcagctc tcctggagta gtgaataatg agaaacaggt
     3241 gattgaggga atcagtgcga ttccttatat tcatggattt aatattttcc gagatttgga
     3301 aactctgttt caactcccag taacgattga aaatgacgcc aactgtgccg gaatggctga
     3361 attttatgaa ggtgctgccc gtgattatca agatgttgct tttgttgtgg caggaactgg
     3421 gattggcggg gcgttgttca cgaaaggaca aatcaataaa ggggcacatc tttatggtgg
     3481 agaatttggg ttgatgttct taaatggcga taagacattc agtaaacttg gtacagcggt
     3541 acagatggct tggcgttatt gtgaacgcaa aggtctcgat aaaaattctt ataccggaaa
     3601 agatgtcttt gaattagcag aaactggaga tgcaatagca aaagaagaag ttgagagttt
     3661 ttatacgtat cttacaaagg gattattcag tattcaattt tcgtttgatc cagaagtgat
     3721 cgtattaggc ggcggtgttt ctgcaaaaga agggctcatt gatgagatca aaagccgaat
     3781 gaagaagctg acagaacaat tcgatctgca tgatttcgaa ccgcagattt tattatgtga
     3841 atacagaaac gatgctaatc ttgtaggtgc agcagcaaac tatctcgctg tacgtcaagg
     3901 ggagaattaa gagctttaga gaaggtgcag cttgcataaa agaagtgaat gaaatgaata
     3961 ggagattttt acctaaggaa tgttttattt gctgcagtat gataaaatat ctgagaggtg
     4021 aaaatgatgt caaaaaaaaa taaagatgtt gaagtgcgta tagaagaagt gaaaaaagat
     4081 atcaaaggta agaattatga agtcactcag ttatttatag ggaaaaaaat gattggtgag
     4141 atcttagcct atgggccaaa agaatatgaa attttctttg gtgaagaaga ttttggaaaa
     4201 gaaaaaagtc ttgaaaatgc gatagaaaca gtgattcgac actggaatct gcacgaataa
     4261 aaaaatatca aaaaaacttt caataaaggg ttgcaaaaat ctggaaaatt aggtattata
     4321 actaagtcag gttgatacaa acctttcgga ggagtagcga agtggctaaa cgcgacggac
     4381 tgtaaatccg ttccttaggg ttcagtggtt cgaatccact ctcctccatt tcattggggt
     4441 atagccaagc ggtaaggcaa gggactttga ctccctcatg cgttggttcg aatccagcta
     4501 ccccagtata ccacaatcgt aagctctcgt tagttcgaga gctctttttt ttgctttgct
     4561 accttgttcc ttgaagattc tgctgattta agatagagtt aagttaacta caagaattgg
     4621 agggatccac atgaaatgga aagaaaaagt agacgaagca gctgaaaagt tatatgattt
     4681 ggtcaaatca gaaaaataca atattgaagt gaatattccg aaaaaaggcg gaaaagcggt
     4741 ccaggtaaaa tcaaaacgcc caacgaatca tacaaaaaaa tggatggcta aaaaccgcta
     4801 ataataacaa aagatccata agtcggcatt ttcatcgatt ttagggtctt ttgttatttt
     4861 ctaggttgaa catatctaaa aaattgtttc tgacaatagg agtaggaaga acgatgtgtg
     4921 tctaaatcct ctgttgatgg atagaaacct ttatcttttt cagttgtgaa tcaggtatat
     4981 accagttata atagaagaaa cgaaaggacg ggtgtacgtt atgtcgaatc aattacctgt
     5041 atatatacag attcatgacc aattaaaaaa tgaaatcgaa caaggtatat ggaaagtcgg
     5101 agaccgtctt ccttcagaaa gagaattagc agtgaaattc aatgtcagcc gtatgacgtt
     5161 gagacaagca attcaaaact tggcagatga aggaatctta gaaagaaaga tcggttcggg
     5221 tacatatgtt gcgcgagaaa aagtacaaga aaaaatgtct ggtgcaacaa gttttacaga
     5281 aattatggaa tcgcaaaatc gagtaccgtc tagtcggaca atctcttatt tcttgacttc
     5341 gccaagctca agtgaaatgg aaaaactaaa cttgagtaaa gatgacacga tcttgcgaat
     5401 ggaacgtatt cgtttcgcag atgatgtccc tatttgtttt gaagttgcca gtgtgccaca
     5461 aaagctgatc gatggctaca gcaaagcaga gattactgaa tcgttgtatc gcacgctaga
     5521 agaaaaaggc gggaaaaaga tcggtccagc gaatcaaacg attactgctg tgttggcttc
     5581 tgagaaaatt gctgactatt tagaggtaaa acgaggagat gcagttcttc gtttgcgcca
     5641 gatttcttat tttgaagacg gtacgccgtt tgagtatgtg cgtacgcaat acgtaggaaa
     5701 tcgttttgag ttttatttgg aaaaataaaa aacaggtgat ccctgattcc ttacttagaa
     5761 ataaaagaga atcagaatca cctgttttta tatatgatca gttaccagtt gctcgtgata
     5821 agaaacagga ccctcttcaa attggacttg tccgttcagc agctcaatga tggattcttt
     5881 aaagtggtca gcactttttt catctacagc acagatgacg ctaacttgat cagtatattg
     5941 gatttctttt ataaaaaaat gttggagttc aagataattc tgtaattttc ctagttgcgg
     6001 atacgtgatc atcacagaga tctcctgttg caaggtacct tctacgattc caatgtcaga
     6061 caatgcttga gagacagcat gagtgtatgc tcgtattaat ccgcctgtgc cgagtttcgt
     6121 tcctccaaaa tatcgagtga ctactgcagc tacattgatc agttgatttt ttttgaggac
     6181 ttccaacatc ggtacgccag ccgttccact tggttctcca tcatcgctgc ttcgctggat
     6241 ttcatttttt tctccaatga tgaaggcgct gcagttatgg ttcgctttcc aatgttcttt
     6301 ttttatttct tgaatgaaac tttttgcttg gtcctctgat tctattcgtc taagagaaca
     6361 gataaagcgt gattttttga tctcaatctc actttgtcca ttttctttta ttgtacgata
     6421 gcgctcgatc atcttgtcac ctcttttttt cattttcttt attcagtata aataaagttc
     6481 taggttgttt caacactgta acggttgcgc aatcgctgta gtatgcgata ataaagacaa
     6541 aggtaggtgg atctggggat gcaggaacga atgaaaaagt acgatgcaat tacgcattat
     6601 ttgaagaaca atggcggctc tcaagtaacg ttgaccttta cccaatttga cgaactgctg
     6661 tttcccagta acggacttcc caaaacagca agggaaagta cagattggtg ggcaaatgat
     6721 tataaacacc ctgaaaaagg tgcctatggc tggataaatg caggctatga agtagttgtc
     6781 atcaatttgg acaaagaata tgtcgtgttc aataaattag tgaaatctag ttggctattt
     6841 gattaaataa accgcgttta gaaaacgttg acaaatttct ttctttttat ataatagagt
     6901 aacagacgcg aagaaaacgt ttgcgtagtt tgttttaaaa aggagagaaa agtatggcta
     6961 aaaaaacaat catgcttgta tgttcggctg gtatgagcac aagtttacta gtaacaaaaa
     7021 tgcaaaaagc tgctgaagca aaaggaatcg actcggatat tttcgctgtg tccgcttctg
     7081 acgcagataa caatcttgca aataaagatg tagatgtact gttattagga cctcaagttc
     7141 gctttatgaa agctgatttt gaaaaacgtc tggaaccaaa aggcattcct ttagacgtca
     7201 tcaatatggc tgattatggt atgatgaatg gcgaaaaagt gttagatcaa gcaatcacgc
     7261 tcataggtga ataaatagaa cagtataact atcgaacaag cgatccaaaa aatagatcgc
     7321 ttgttttttc gtatattttt ttagggcgaa taatatgaat catttttggg gtagaaggct
     7381 tctgacgaga gatattaaag caatagactg tgaggaggta aatttaccaa attatgagaa
     7441 gatcgcagca gtggaacgtt tgggcaactg gattctttgt catcgctgtg gaatgaggac
     7501 gtccgtcatt gatggacagc ttgcagatta cggttatttt tgtacacatt gtctatattt
     7561 agggcgatgc gacactcaac aagatttata tttgtttgat cagcctgaag caaaacctcg
     7621 agaagtcgtc tttttttgga caggacagct tactgaaaaa caaacggaaa tcgcaaagag
     7681 aatactttgc cattcagaaa aaagacatca tcttatttgg gctgtaacag gtgcagggaa
     7741 aacagagatg ctttatccta ttttagtgga aactttaaaa gctggaggaa gagtagcgat
     7801 ttgtacaccg cgagtcgatg tttgtaatga attgttttta cgctatcgtt atgtttttcc
     7861 agaagaaaaa atcacgttgc tccatgggaa tacagcaacg gcttatacct ttacttcttt
     7921 tgttatctgt acgattcatc agctataccg tttttatcgg tcctttgatt taatagtgat
     7981 cgatgagatc gatgcgtttc cttataacgg ggatgcagga ttagctcatg ctgtgcaaac
     8041 agcgattaaa ccagacggac gattcatcta cttaagtgcc acacctgata aaaagttact
     8101 aaaggaaatc aagcagacgt ttgacatcca caaacttgct ttgcgctttc atcggcgttt
     8161 acttcctgaa cccgtcttgg ttttcatgaa taattggcga caaaaatgca agaacaagaa
     8221 gaagataaaa tctttggtgc ggatcattca tcaattaatt aaagaaaacc atatcctttt
     8281 attttgtcca agtatcacga tgatgcatcg tttgaaagcc acacttcaag aggctcttcc
     8341 gagatatgag atcacagatg tttcttcaag agacaatcag cgggcggaaa aagtccagaa
     8401 aatgcgagaa gcaaaatatg acatactgtt aaccacgatg atccttgaac gtggagtcac
     8461 atttgaaaga atatccgtta tcgttttagg agcggatcat ccggttttta ctaaatcttc
     8521 tttggtacaa atcgcaggaa gagccgatcg taaagggcct tatacgaaca gtcaagtata
     8581 ctttttctat gaagaaaaaa ctgcagctat tcgaaaagct tgtcaggaaa tcaaagaaat
     8641 gaatgaagaa gggaagaaga tgctgtgagg tgcagttgct gcagtaaaga tatcgtccaa
     8701 aatttaacat tcagcgagat tattggtgtt tcgaaataca gattacctca gacttgttca
     8761 gtttgtcgcg cacaatttca tttattggaa acaagaggct gttctggctg tcaaaaacca
     8821 atccatttca atctagagag tacgaaaaag aaagtaaagt ctattcccgt ttactgcagt
     8881 gactgtcaag aatggaagaa gaaatatccg acctatgatt ttcagcaccg tgcctttttt
     8941 gtgtacgatg aaaatatgaa ggcctggctc aatcaataca agtttatggg agatatccga
     9001 ctagcaggca cctttgcata taaatggacg cagataaaaa aagaataccc gacatacatc
     9061 tattgtccaa tacctttatc tgaaaaaagg cgaacagaaa gaggattcaa tcaagtaagc
     9121 gagatgttga aagcagcaag aattcctttt cagatgctac tcaaaagaga gaagcatctg
     9181 ctggctcaag caaaaaacac gcgaaaggaa agattggaga tgcctcaacc ttttgaactt
     9241 gcagtcacct tttctaagat acaaaataaa aaaatattga ttattgatga tgtctataca
     9301 acaggacaga ctcttttcca cgcagcagat tgtttgcagg tcgcacagcc gaaaatcatc
     9361 cgaaccattt ctttagcaag atgaattaga tgtggaaatt tgatagcgat ttaatttgca
     9421 aataacctag agttcgatat aatagaatta agaagaggga aagagtggtc cttctccttc
     9481 ttgattgtgg tagacgaaag gggtaatttt tatgtttaga tataatgtac gcggcgaaaa
     9541 tatcgaagta actgaagcga ttcgcgacta cgttgagaaa aaagtaggca aactagaacg
     9601 ctatttcagt gacgcacccg atgcaacggc gcatgtgaac ttaaaagttt atacagaaaa
     9661 aacagcaaaa gtggaagtaa cgatcccatt accttatctt gtattacgtg ccgaagaaac
     9721 ttctccagat ttatacgcaa gtatcgattt ggttgttgac aaattggaac gccaaatccg
     9781 caaattcaaa acaaaaatta atcgtaaatc ccgtgaaacg ggcatgaata ctgcagatgc
     9841 tactgcttta ttcaatgaag aggacgaaaa cgaaaatgat tctgaattag atatcgtacg
     9901 tacgaaacgc ctttcattga aaccaatgga tagtgaagaa gcagtcttac aaatgaacat
     9961 gctgggacat aacttcttta tttttgaaga tgcagaaaca aatggtacaa gtatcgttta
    10021 ccgccgtaaa gatggaaaat acggactgat tgaaacagac tgatcagcct tacttaaata
    10081 acaacgaaaa cttgcagcag ctactgacag ctgccgcaag tttttttgag tttcctcctc
    10141 ttttagatga aatatgaata aattatgaag ttcttacttt aatacgctta agatatttca
    10201 attatctata actaatgata aaatgaaaag taaagtctac atagtctgga taatacacag
    10261 aaggaaaagg agattacaca acgcatggcc aatttgattc gttcacttat cgaaaacgat
    10321 aagaaagaac taaaacgttt agatagcatt gcaaaaaaag ttgaagcata cgctgaccaa
    10381 atggcagcat taactgatga acaattacag gcaaaaactg ctgaattcaa gggcagatac
    10441 caaaaaggag aaacattaga tcaattactt ccggaagctt ttgcagtcgt ccgagaagca
    10501 gcaaaacgtg tattggggtt atatccacac cacgtacaat tgatgggtgg aatcgtgctt
    10561 cacgatggaa atatccctga aatgcgtact ggtgaaggta aaaccttgac cgcaacgatg
    10621 ccagtatatc taaatgctct gactggcgaa ggggtacacg tagttaccgt caatgaatat
    10681 ttagcaaccc gtgactcgac ggaaatgggc gaactttata atttcttagg attaacagtg
    10741 gggttgaata tcaactcaaa atcttcggat gaaaaacgcg aggcttataa ttgtgatatc
    10801 acttatagta cgaataatga attaggattt gactacttga gagacaatat ggtcgtttac
    10861 cgccatcaaa tggtacaacg tccgctgaat tatgccattg tcgatgaagt tgactcaatc
    10921 ttgattgatg aagcgagaac gccgctgatc atttcagggc aagcagaaaa atccacagct
    10981 ttatatacaa gagcagacaa cttcgtgaaa cgtttgaaag aagaagaaga ttataaaatc
    11041 gatgtacaat caaaaacaat cagtttaacc gaagcaggga tcgaaaaagc agaagaaaca
    11101 ttcggtttgg ataacttata cgacattgaa aatactgcat tgacccatca cttggatcaa
    11161 gcattgagag ccaactatat catgctttta gatatcgatt atgttgtcca agacggaaaa
    11221 gtactgattg ttgaccagtt cactggtcgg atcatggatg gacgccgtta ttcagatgga
    11281 cttcaccaag caatcgaagc taaagaaggc gtagagatcg aagacgaaac gaaaaccatg
    11341 gcgacgatca ctttccagaa ctatttccgt atgtacaaga aacttgcggg aatgactggt
    11401 acagctaaaa cagaagaaga agaattcaga gaaatttata acatccaagt ataccagatt
    11461 ccgacaaatc gtccggttat ccgtgatgat cgtccagatt tgctttaccc aacattacaa
    11521 agcaagttcc acgctgtcgt gcaagatatc aaagatcgct accgcaaagg tcaacctgtg
    11581 ttagtcggaa ctgttgcggt tgaaacatct gagttattat cagatatgct gaatcaagaa
    11641 cgtattcctc atgaagtgct gaatgcgaaa aatcacttta aagaagcaga gatcatcatg
    11701 aatgctggtc aaaaaggtgc tgtgacgatt gctaccaaca tggctggtcg gggaacagat
    11761 atcaagttag gacttggggt aagagaactt ggtggtctag cagttatcgg gacagaacga
    11821 catgaatctc gtcgtatcga taaccagctt cgaggacgtg caggacgtca aggagaccct
    11881 ggtatgtcac agttctactt gtcattggaa gatgatttga tgaaacgatt cggttcagaa
    11941 cgaatcaaat ctttcttaga tcggatgaag atcggtgagg aagatgctgt tatccaaagc
    12001 aagatgctga cgaaacaagt agaatctgca caaaaacgtg tagaaggaaa taactatgac
    12061 acacgtaaaa acgtcttaca atacgatgat gtgatgcgtg aacaacgtga agtaatctat
    12121 aaacagcgac aagaagtaat catggaagaa aaaagcttat ccaagccact aatgaacatg
    12181 gtcaaacgga caatcagtcg tgtagtagat gcgcatacac aattagagaa agaaaattgg
    12241 aatttggaaa gtatcatcga ttttgccggc agtactcttg tccatgaaga tagtattact
    12301 cttgcagata tcgaaggaaa aacagctcag gagatcaaag agtatctaaa cgaacgagca
    12361 caagaagtat ttaataccaa agcggctcaa ctaaatggtc cagagcaact gttggaattt
    12421 gaaaaagtcg tgatcttgcg tgttgtggac tctaaatgga cagaccatat tgatgcaatg
    12481 gatcaattac gtcagtcaat cggcttacgt gcttatggac agaacaatcc attagtcgaa
    12541 tatcaaacag aaggctataa gatgtttgaa gatatggtag gcgccatcga gtatgaagtc
    12601 actcgaatct ttatgaaagc tgagatccgt caaaacgtac agcgtgaaca ggtttctcaa
    12661 ggaacagctt ctcactcaga agacggagat gccaaagaaa atagtaatac tagcgcgaaa
    12721 aaacagccaa tccatgtgaa caaggtcgga agaaatgatc cttgtccttg tggaagcggg
    12781 aaaaaatata agaactgtca tggaaaaaat gcaaactaat ttctgacagc aggattggtg
    12841 agacaaattc tttttttaag aattcgtctc accttctttc tgttatttgc tgattctgtt
    12901 agactagata taatgaaaaa agtatagtga aagaaggaaa aaacatggaa atatccgaaa
    12961 ttcgaaatct cctgacagaa atgcaggaaa aggtcacaag cttcaggagg tctctttgac
    13021 ttagaccagc tggaagaaga cattgcggaa gcagaagcaa aaatggctga acctggtttt
    13081 tgggatgata gcgaagctgc tcaaaaagtg atcagtgaaa acaatgcaaa taaagaaact
    13141 tatgatcaat tccattctct tgctgaagaa ctggaagaaa tcgaattgat ggtggagatg
    13201 ttgcaggaag aacctgatgc agccatgcaa gaagaagcag aaacacgtat caatgcgcta
    13261 gatgaaaaaa tgaagacata tgaattatct atgttgttag atggtcctta tgatcgaaac
    13321 aacgccatcg ttgaattgca tccaggggct ggcggaacgg agtcccaaga ttggggcagt
    13381 atgcttttac gtatgtatac acgctgggca gagcagcatg ggtatcaagt agagacacta
    13441 gattatcaag caggtgatga agcaggaatc aagagtgtga ccctactgat caaaggacac
    13501 aacgcatttg gttatctgaa atcagaaaaa ggtgttcatc gccttgtacg tatctcgcct
    13561 tttgactcag cgaaacgacg tcatacttct ttttgttcgg tagatgtgat gccagagctt
    13621 gatgaaacga tcgatataga aatcaatcca gatgatttga aaatcgatac gtaccgagca
    13681 agtggtgcag gtggtcagca catcaataaa acagaatctg ctgtacggat cactcacttg
    13741 cctacaggaa cagtagtagc tagtcaggca caacgttcac aattgaaaaa ccgtgagcaa
    13801 gcgatgggga tgttaaaagc taagctttat caattagaag tggaaaagaa agaacaagaa
    13861 gcagctgcac ttcgaggaga acaaaaagaa atcggctggg gttcacagat ccgttcttat
    13921 gttttccatc catattctat ggtaaaagac catcggacaa acgtagagac aggaaatgtc
    13981 caagcagtta tggacggtga ccttgattta tttattgacg gctatttaaa aatgcatcta
    14041 caataagaac ccattgttaa attgaaataa aattgtaaga ttttgcaagc atgttgaaac
    14101 attgccatgc tataatagct aaagactgaa aaagatatgg agagaacgcc atgattgaaa
    14161 tgaaggatgt aatgaagaag tactcaaatg gtacgaccgc tatccgcaat ctctcaattg
    14221 tcatcgaaca aggagagttt gtctatgtgg ttggtccgtc tggagccggt aaatctacat
    14281 tcatcaaatt gatgtatcga gaagaaaaag cttcaaaagg aactttgaac gtcgcaggtc
    14341 atgatctaat agcaatcaaa aaccgcgaag ttccttatct acgcagagaa atcggcgtgg
    14401 tgttccaaga ctataaatta ttaccaagaa aaacagtata tgaaaatgtt gcgtacgcta
    14461 tgcaagttat cggcagaaaa cctcgggata tcaaaaaacg agtaatggaa gtgctggacc
    14521 ttgttggttt gaaacacaaa gttcgagtct ttcctagcga attatccggt ggggaacagc
    14581 aacgtgtctc tattgctcgg gcaatagtga atacgcctaa agtacttatc gcggatgaac
    14641 cgacaggaaa tctggatcca gaaaactcat gggaaatcat gaaattatta gatcggatca
    14701 atgcccaagg aactactgtc gtgatggcta cacataacag tacgatcgta aatacgattc
    14761 ggcaccgagt aattgctatc gaaaacggcc gcattattcg agatcaagcg gaaggggaat
    14821 atggatacga tgattagaac ctttttccgc catttattag aaagtatcaa gagtttgaaa
    14881 cgtaacggct ggatgacagt tgcttcagtc agtgcggtaa cgatcacatt gactttagtc
    14941 ggtgtattca tggcagttat catgaacgca actaaattag ctcaagatat agaaggaaac
    15001 gttgatgtct ctgtattcgt agatatcggt acaaaagacg aagagatgaa aaagctagaa
    15061 aaagaattga atgacttaga tcatgttaaa aaagttgaat tttcaagcaa agaacaggaa
    15121 ttgaaaaaaa tccaaaacga aatgggtgat tcctggaaat tgtttgaagg tgacaataac
    15181 ccgttatatg atgtgtatgt agtaagtgct actgatcctt cttatacaaa acaagtagca
    15241 gaagaagcag cagatttgaa gaatgtttct cgttctgatt acggtggagc ttcctcagat
    15301 cggattttcc aaatcgctgg tgcagttcgg acatggggat tagttgcagc ggcattgctg
    15361 ctgttcgtag cgatgtttct catttcgaac acgattcgga tcacgatttt gtctagacag
    15421 cgtgaaattc aaatcatgcg tttagtcggt gcaaaaaatg gatatattcg ctggccgttt
    15481 ttccttgaag gtggctggat cggtctgatc ggtgcgattc ttccgatttt gatcatcaca
    15541 tttggttatg catggttttt ccaattgatc aatccatcat tattacgttc ccattattca
    15601 ttgattcatc cacaaaatat cgtttggaaa atcaatcttt taatggtcgg cattggtgtg
    15661 atcattggtt cacttggctc gatcatttct atgagacgtt tcttgaaatt ctaataccac
    15721 aatataaata aaatgagaaa gttggacagt ttcgttccag ctttcttttt ttattggcta
    15781 ggagaataga aggaagcggc ttttttccct ttactttaaa aaacaacttg ggtattctta
    15841 agttggtaga agaagaggtg aagccgtgaa aattatcgtt gacaattttt ttacgatcct
    15901 tttgattatg aatattttgc tttcctttat catcgttttt cgagaaagaa aggaaactgc
    15961 tcaaacatgg gcatgggtgc tcgttttgat gttcattcca gttgtaggat ttattttata
    16021 tattttttta ggaagaggga tttccaaaga gaaaatcttt gatttgaaga tccaagggaa
    16081 aatcggtaag aacctggaaa ttgaagaaga caaacaggca ttgatgcgag ggctttatcc
    16141 tcatccgcct actggacaag tagatgtgaa acaattgatc tatatgctca cggtatttga
    16201 aagtacgctc tatacgacta aaaatgaaat cactttattt acagatggtc gtgaaaaatt
    16261 cgatgcgctg attgaagata tcaagcaggc agaagaccat atccacttcc agtattatat
    16321 ttatcgcagt gatgcattag gtgaagaagt tcgagatgca ttgacagagg cggcaagacg
    16381 aggggtaaaa gtcagagtcc ttcttgatgc atggggttcc acacaagttt cttccagctt
    16441 ttttcagaat ttgaaaaaag caggcggaga agtcgcattt ttctttcctc tatttgttcc
    16501 gtatattaat cctcggatca attatcgcaa tcatcgtaaa atcgtagtta ttgatgggaa
    16561 aatcggatat acaggtgggt tcaatgttgg aaatgaatat ctaggattgg taaagaaatt
    16621 tggttattgg cgagacaatc atttgcgtat ttacggagaa gcggtgtata ccttgcaaaa
    16681 ccgttttttg atggactgga attctcagca tacaaaagaa gtaggctatt ctcccaaatt
    16741 ttttccgtcc attcactcta ctggtgatat agctgttcaa atcgtgacaa gcggaccaga
    16801 taccgaacat gaacaaatca aaatgactta tttgaaaatg atttcgttag ccaaacggga
    16861 gatattgatc caaacgcctt attacatacc ggatggatcg attcatgaag cactgaagtt
    16921 ggctcttctt tcaggcgtaa aagttcatat ccaaatacct aacaaaccag atcatcttct
    16981 cgtttactgg gcaacgtatt ctttcgctgc agagctaatc gagtatggag caaggatcga
    17041 aacatatgag aatggattta ttcatgcaaa gacgatgatt atcgatggtg gcattgtttc
    17101 agttggctca gcaaatatcg atgttcgttc atttcgcttg gactttgagg tcaatacact
    17161 ggtttatgat gaacgaatgg cttcacgtgt aagaaaagct ttttttgaag actcgaaaat
    17221 ttctacgcat ctcacaaaag aaatgtatga aaaccgagga ataatcatca agatgaaaga
    17281 aggattagca agattgattt cgccgctcct ttaaaacata aaaaggacat gagaaccatc
    17341 aacagatgga gatctcgtgt ccttttctaa cggggccatt tctgagagta cgtcagaaat
    17401 ggagaaagcg tctcgcatga gcgaaattaa ctttggggta tctcttgttt tttggggaaa
    17461 gcaagaaata agctaatata tgctattaaa agagacacat ttcaaagcca gtatagcata
    17521 gaaactttta atgagatagt tttttttaca attatgagaa tttattttgt tatttaatga
    17581 ttttgattcg ttttcatcaa gtaatgaaaa tgataaaata aacatacttt aggattaaag
    17641 gagatttcta atgaaaaaac ttaaattatt cttaccaata ataggattag ctattctttt
    17701 atcaggctgt tcaaagtgga ttgatcgagg agaatccatt acggctgttg gctcttctgc
    17761 tttacagcct ttagtagaaa cagttgctga aacttaccag acagaaaatc ctggaaaatt
    17821 catcaatgta caagggggcg gaagcggaac tggtttgtct caagtacagt caggagcagt
    17881 cgacatcgga aactctgatt tgtttgcgga agaaaaatcg gggatcgatg cctcagcatt
    17941 agtcgatcat cgagtagcag ttgttgggat cactcctatt gtgaacaagg atattggcgt
    18001 aaaagacatc agtatggaaa acctgaaaaa actatttaca ggcgagatca aaaactggag
    18061 tgaagtcggt ggaaaagatc agccagtagt tatattgaac cgcgcttcag gaagcggaac
    18121 ccgtagcacg tttgaaaaat gggtattgga cggagaaaca gctgttcagg cacaagaaca
    18181 agattcaagc ggaatggtgc gccagatcgt tgccgacaca cccggtgcaa tcagctatgt
    18241 tgctttttct tatgtttccg atgatgtaga tacgttgaat gttaacggtg tagaaccgac
    18301 agatgaaaat gtgacaacga acaaatggac gatttgggca tatgagcata tgtacacaag
    18361 agatcatcca aaaaaactga caaaagaatt tttagactat atcttatctg atgaagtaca
    18421 agataacatc gttggagaac taggttatat ccctgtttct catatgaaag tcgaacgtga
    18481 ttggcaagga aatatcgtta aagagtaatc tttacacaaa atttacaaac ttagctaaac
    18541 ttttacatgt tattaaaacc aaattgatgt tcaatttgta cactgagtga gtatgttaaa
    18601 cctgattaag ggggagtcat ctgtggaaga tgtgaaaaaa gtattattaa caaaatcaaa
    18661 acgttcaaaa atggaacagc gtggaaaatt catcagtttt ttatgtatcg cgctgatcgt
    18721 gttggttgta ttatctattt tttattttgt agctagtaaa ggattggcca ccttttttac
    18781 tgataagatc aatgtatttg attttctgtt tggtacagat tggaatccaa gccaaatcgg
    18841 agcaaatggg aaaccaatgg taggggcatt gcctatgatc acaggttcgt ttattgtgac
    18901 ctttttatct gctatcgttg ccacgccatt tgccattggt gcagcagtat tcatgacgga
    18961 gatctctcct aaaaacggaa cgaagatctt acagccggtc atcgagctgc ttgtaggtat
    19021 tccttctgtc gtttatggtt ttatcggttt atcagttatc gtgccttttg tcagaagtat
    19081 ttttggcggg actgggtttg gtatcttagc tggtacattt gtgttgttcg ttatgatctt
    19141 gccgactgtc acaacgatga ctgttgatgc attaaaggca gtgccacggc actatcggga
    19201 agcttcatta gcgcttggtg ccacaagatg gcaaacaatc tacaaagtcg tgctacgagc
    19261 agccgtgccc gggattttaa cagcagtcgt ttttggtatg gctcgtgcgt ttggtgaagc
    19321 cttagctatc caaatggtca tcggtaatgc agcattgatg ccaacaggtc ttactactcc
    19381 tgcttctaca ttgacgtcca tcttaacaat gggaatcgga aatacaatca tgggaacagt
    19441 tgaaaataat gtgttatggt cattagcgct gatcttgcta ttgatgtcat tattctttaa
    19501 tatcgtgatc cgtatgattg ggaagaaagg agagctgaaa taatgaacgc aaaacgctca
    19561 gataaaattg ctacaggtgt tttatatata gtttcgggga tcatcgtatt gattttagca
    19621 gctttactac tttatatctt agttcgggga ataccgcaca tttcatggtc cttcttgacc
    19681 gaaccttcca aagcctacca agaaggcggt ggaatcggga tccagctatt taactcgatt
    19741 tatttgcttt tgatcacgat gatcatcagt tttcctattt ctttaggtgc gggtatctat
    19801 ctttcagaat atgctggaaa aaactggcta acagatgtga ttcgcacgtc gattgaaatc
    19861 ttgagctcgt tgccttctgt cgttgtcggt ttattcggtt tcttggtttt cgttattcaa
    19921 ttccagtatg gtttctcgat tatttctggt gcattggctt tgactgtatt caacttgcct
    19981 cttttgacta gaacagttga ggagtcttta caagcggtcc attacacaca acgagaagca
    20041 ggattggcct taggtttgtc acgctgggaa acagtgataa aagtggtggt tccagaagcg
    20101 acaccaggga ttttgactgg tgtgatccta agttctggaa gaatttttgg tgaagcagct
    20161 gcattgatct atacggctgg acaaagtgca cctgcgttag attttacgaa ctggaatcca
    20221 ttaagtgttt caagcccgat cagcattttc cgtcaagctg aaacattagc tgtccatatt
    20281 tggaagatca atacagaagg cacgatgcct gatggtgttt ctgtttcggc tggcgcttct
    20341 gccgtgttga ttattgctgt tttactgttc aacttcggcg ctcgtgcgat tggcaaacga
    20401 ttataccgaa aaatgacttc agcctaagga gaagaagcaa aatgaaaaaa tataactgga
    20461 atgaaacaaa tgtcatcacc atgccaaaag agaaagaagt agcactatat acggatgatc
    20521 tacatgtctg gtatggcgac aatgaagcaa tcaaaggagt ggatctggaa tttgaaaaaa
    20581 ataagatcac tgcactgatt ggtccttccg gctgcggaaa atctacgtat cttcgttcgt
    20641 tgaatcgaat gaatgatggg atcgctaata cgaaaatcac tgggaaaatc atgtatcaag
    20701 gcgtagatat taatgcgcct caagtggatg tttatgagat gcgcaaacgg attggcatgg
    20761 tttttcaacg tcctaatccg ttcagcaagt cgatttatga caatattaca tttgctttga
    20821 aacagcacgg cgaaaaaaac aaaaagaaat tagatgaaat cgttgaaaca agcttgaaac
    20881 aagctgcctt gtgggaccaa gtaaaagaca gcttggacaa aagtgccttg gctttatctg
    20941 gcgggcaaca gcaacgttta tgtattgctc gtgcgatcgc gatgaaacct gatatcttgc
    21001 tgctagacga gccggcaagt gctttggacc ctatttcgac aggaacggtt gaagaaacac
    21061 ttgtaaactt gaaagaagac tatacgatca tcatcgtgac gcataacatg caacaagcag
    21121 ctcgtatcag cgactatact gcctttttct atttaggaaa agtaatggaa tatgacaaaa
    21181 ctaacaagat atttaccaga ccaaaaattc aggcaaccga agattatgtg tccggtcatt
    21241 ttggttaaga cggaggcctc aacatgacaa aagagattat ttcatctaaa gatcttcatc
    21301 tgtattatgg aaagaaagaa gcactaaaag ggatcgatct aagcttcaat caaggggaaa
    21361 tcactgcgat gatcggtcct tctggttgcg gaaaatccac ttatctgcgt tctttgaacc
    21421 ggatgaatga cctgattccg aatgtaacga tcaccggaag tgttttatat aaagacaaag
    21481 acatctatgg tccaaaaaca gatgttgttg aactaagaaa agaaatcggc atggtattcc
    21541 agcagccaaa cccatttcct ttttctatct atgaaaatgt catctatgga ttgaaattaa
    21601 aaggggaaaa agataaaaaa gtccttgatc aagtagttga agaaagcctg aaggcagcgt
    21661 ctgtttggga tgatgtgaaa gacaaattgc ataaaagtgc tttatcttta tccggtgggc
    21721 agcagcagcg tgtatgtatc gcacgtgttt tggcagttaa tccggaaatc atcttattgg
    21781 atgaaccaac aagtgcgctt gatcctgttt ctactgggaa aatcgaaagt atgctgttag
    21841 aattaagaga gcggtatacg atgatcatcg tgacccataa tatgtctcaa gcttcacgta
    21901 tttcagataa gacggcattc tttttagatg gtcacttgat cgaatacaat gatacgaaga
    21961 agatattcat gaatccagaa aaacaagaga cagaagatta tatttcaggt cgatttggat
    22021 aaggaggaaa cggaatgcta cgtacacaat ttgaagaaga attgctgaat ctccataatc
    22081 agttctacga aatggggatg atggtgagca gcgccgtgca caagtcagtt cgtgcataca
    22141 tcaaacacga taaaatctta gcccaagaag ttatcgaaaa cgatataaat atcaataaca
    22201 tggaaacacg cttggagaag aaaagttttg aaatgatcgc attgcaacag ccagttacaa
    22261 ctgatttgcg tatgatcatt acggtcatga aagctagctc ggatttagaa cgtatggccg
    22321 atcatgcggt gtctgttgct aaatccacga ttcgcgtaaa agggcagacg agaataccgg
    22381 aaatcgaaaa agagatctcc gatatgtccg attatgtgaa aaaaatggtg gacaacgtcc
    22441 tagttgctta tgtcaaaaca gacgaaaaag acgcacgtac gatcgccaat atggatcaaa
    22501 gagtcaatga ctatttcaat cgaatctata atgcaacaat caaaaatatg caggaaaatc
    22561 ctgagacagt catcagtgga acagactact tgaatgttgc cagttatcta gaacggatcg
    22621 gagactatgt gacaaacatc tgtgaatgga tcgtctactt ggctacaggg aaaatcactg
    22681 agttaaatac gaaccacaat gagattttct aaactgaagc tgtgattatg ccgtttgttt
    22741 aggaaaagag gttgtgaaat cagcggttgt gaaaaagctg ttctgcatca agtaataaga
    22801 acagtcaaac aaaaaatagc tcctcatatt tttcgtttgg ctggacttat taccgcagat
    22861 gcaagctttt gaacaccgtt tattcgggaa agggtttgtg acaaaacatg tcacaaaccc
    22921 ttttcatcat ttcatagaaa ggataaagaa tgcaaaaaaa aattagcatc atcactatat
    22981 tttttatttg tgtgcttact tcatgggtta ttcagcatca agccgcttta tatgaagtac
    23041 ctgtcatcga gatagaaaaa gtacgaataa aagaggatca tcaattgatt acaggaaaac
    23101 tgatgaacac ggatcaaaaa ggagaatcga tttatttatc ggttccttat caaaaaaatc
    23161 aaatagaaac agcaaatata aaagcgagac aaatgtatct agtccaaatg catggagatg
    23221 aatggcagct agtagcaaaa aaaaatgatg gctggctttt cttcttcaca agtatcttca
    23281 ttggcgccat gttgcttatt ggtggaaaac agggtctatt tgctttagga agtctcatca
    23341 tcaatatcgt tttactttta cttttgttgc tcatctttac taaaacagaa gggatttctc
    23401 tattaaatat atctatcctg ttcgtcgtta tagggatgat tattagcata ttggctttag
    23461 atggatggaa tagaagcagt ataatcaaaa tttcagctgc agttacgagt gtttttttag
    23521 cattttttat ttgtcttttg acaatgaatc attggaaaga ccaaggactg cgctatgagg
    23581 aactgaatta tctgacacga ccgtatcgtt ctgtttttct ttctagcgta ctgattggaa
    23641 cagctggagg aacactcgat accgttatca cagtcattgc gacattagaa gaattgacta
    23701 agcgacaacc agaaataaca gcgaaacagt tatggctttc aggaaggcaa gtgggtcgag
    23761 atgtgattgg ctcgatgacc aatatccttt tgttcgtcta tatcagtggg tcagttccat
    23821 cgcttctttt atatttagga aatcaatggt cgttcaaaga aacgatagaa cagcatttat
    23881 cattggaatt tttacgcgtc attgctggca gcttaggaat agtattatct atccctatct
    23941 cggtcctctt ttttctgact gtaaggaggc tgaaacgatg aatgtactta ttatacttgt
    24001 tgggatttta gcaatcagcg tgctacttat tggcggtacg caaggtatgt atattctttt
    24061 aggcttattc atcaatttag ggatcttttt cctgttgtta ttcggttatc atcagaagtg
    24121 gccgatactg gcattgtcgg tcataggatt tttattgatt gctgtggtta tcttattttt
    24181 tatcaacggt tataatctaa aaatgcgtgc agcttttgtg agtatactga tctttttatt
    24241 ttgtttccta ctattgcttc ctatcactga ttttttagca atccaaggtt ttacatcggt
    24301 agaactagaa gaattatctg gcttagataa gactttagcg attgatttta gattacttac
    24361 gagatcgttg cttctgatta gtcttagtgg tgctgtttta gatgcgagtg tagcaatttc
    24421 tagtgggaca ttcgaagtat atcaggcaaa tccgcaatta tctttttacc aattgcgcca
    24481 ttctagtttc gctattgcta aaaaaatatt ggcgtcaaca gtaatgacac ttctttttgc
    24541 attcatggga agttctttag cgttgatcct ctggtttatt gatttagaca ttccttttca
    24601 gcaagtgatc aatgaaaaat catttgttct agaatatacg atggctattc ttaccacatt
    24661 gtccgcacta cttgttcttc ctttaacctg cgtgactaca gcatatctct ttacaaaaaa
    24721 gaaagaacaa ctcaagatgg agtaaaagtt acaacttaag tcctatgaca tttttactga
    24781 atcagtgcat aatatggatg taaggaaaag cttgcaaaca attttgctta aggaggcaat
    24841 ttccatgaaa gaaagagaac gtattttaga tttagtgaaa aaaggaatcc tttcgactga
    24901 agaaggctta gatttattag aaagcatggc aactgaaaaa gacgaaaagc aaatcaaaaa
    24961 agaagcagac aaggtaactg ctagtcataa agaaaaagat caggcaagtc aattgatcga
    25021 taaacttgaa aacggcgaag aagaaatttc tgagccagtt gatccaaagg aaagagaaag
    25081 acaagatcaa gaaaacttag aaaagatctt agatgaacta gcaactgaag cgaataaaac
    25141 ttcggcgcgt ctagatgaag taaatgctca attcgctgac aataaggcag cacgcaatga
    25201 gaaacaggaa gcattgatgc agctgaatac aaaagaagaa ttaggcgagt tgacagaaga
    25261 agaattagca cagcgtcaga cgttagaagc agaaatcaaa gaattagaag ctagtggcga
    25321 tacactgtta gaagaacaat tgaaattaga agcagaatta aaagatatcc gtaagaatca
    25381 atggtctgag aaaaaagaaa cgtttactga taagtttgaa ttgccagatg attggaaaga
    25441 tcaagcaaca gatacactga atcaagtagg cgaaaaaatg tcagaagctg gatcacagtt
    25501 aggcaaattc ttgaagaaaa cattccaaac tgtttcagaa acagtgaatg acaacatgga
    25561 atggaaagat gtcagcttac gtgtccctgg gattgcgaca acgaaatttg agcatgaatt
    25621 ttactatgaa gctcctgcag caagtatcct tgatatcaag gcagcaaacg gaaacgtgac
    25681 gttaaaaacg tgggacagca acgatgtgaa agttgaagct aagatcaaac tttacggaaa
    25741 aatgggtgct gaaccatttg aagcattctc tgaacggagc cagattgagg ttaatgaaga
    25801 tcatatctcc ttccaaattc caaataaacg tgtgcgtgca gaccttgttt tctacttacc
    25861 taaacgagta tacgaccacg cagctattaa acttctaaat ggcaacatca tgattgaaac
    25921 attggaagct aaagatattt atacaaaatc tacgaatggt aacatcatcg tggaccaatt
    25981 aactgcaacg atgctggaag tagaaggtgt aaacggcaat atcgatattc gaaacgggaa
    26041 catcttagat tctattatcg aaacagtaaa tggtacagtg acatttggtg cgacagcaga
    26101 aaacctttct gtgtctttgg taaatggtga tgtacgtctt acgattaaag aagataattt
    26161 gaaaaaagta gaagcaagtt cagtgaacgg aaatgtcaaa gtcgctttac cagatggcat
    26221 cggtttggaa ggacatgcta aaacaagtct tggaagtatc aacagccgtc tgtccaatta
    26281 cgaagtagtt cgtgagaaaa aagaacggac aaaccaaatg ttgcaattcc gtcgtgtttc
    26341 agatggtgaa atcgcgcaag tgcaactttc aacgacaaca ggaagtatct atttaaaaga
    26401 tacagataaa taaagaggtt gtgaaagaga tgttcagcct tgagcaataa gaagcgattt
    26461 tgtaaaatag cttcccctat tttacaaaat tgtgccttat tgccgatagt ctatctcttg
    26521 aacaccgttt accaagagat tatgaacctg ccaattatcg ccgagtcaca aggaataatt
    26581 tcagaaaaag ctcgtcacgt tttatgaata tggtaatttg tgaccgagaa ttttgcagaa
    26641 agacaccgat taagaagaag gttgtcacaa cgattttctt catttaaaat agtagaacag
    26701 aggtgagaca tgatgaaaaa aaagttgaca aaatctaata aaaatgttgt tttgacaggg
    26761 actttagcag gaataggaga atacatcggg attgatccga ctgttatccg tgtgctttat
    26821 gtattcctga gcttagttgc ttttggaagt ccaattattt tatacatttt attagctttg
    26881 attatcccat ctgcgcgttc tgcacgaaca aactatggac ataacaatgc atattatgaa
    26941 aaaaacaatt atcgtgaaca aactcgtgaa caaacaaaaa aacgcaaaga agcggaaaaa
    27001 atcgatgatg acgaatggag tgacttttaa tgtcgtactt tcaacgtatt ttagttaata
    27061 cgttaacatt catatcattg gctgttcttt ttcctgcgat ggtccatgtg agcagtattt
    27121 ggacggccgt tcttgccagc ttcgtattgt ctgtgttgaa tatgtttgtc aaaccgattt
    27181 tgacgatact gtccctgccc tttacattag tcacattggg attgtttacg tttgttatca
    27241 atgcagcgat tcttaaaatg acctcctttt ttgtgggagc agaacatttt agtttttctt
    27301 cattcggtgc agcgttgtta gtagctatct tgatgtcgct tgtcaatgta attgtcagcg
    27361 aacataacag tagaaaataa aagaaagctg tgccgaacta tcggcgcagt ttttttattt
    27421 agaatcagga atgcgttttc tattacattg gtaattaaat aaaaacgttt tttttaattg
    27481 gtagtacttt tggctagaca atgataaaat gatagagaac actctaatag aaatgaggta
    27541 gcatgaatgt cagaagttgt aaaagttagc caattagtga aaaaattatc tttggaaatc
    27601 gtgacaggcg acgagaaaag cttagaccgt gtcatcgtaa ctggggacat ctcacgccca
    27661 ggactagagt tgactggtta ttttaattat tattcccata atcgtttaca gctatttggc
    27721 agtaaagaaa tcagttttgc ggaacggatg atcccagaag agcgtttgat catcatgaga
    27781 agattatgta gtgaagatat gcccgcattt attatttcaa gaggattacc agctccagaa
    27841 gaaatgatcc aggcagcaaa tgaaaatggg atagctgtac tgcgttctcc catctcaact
    27901 tctcgtttat taggagaatt atccagttat ttagatggtc gccttgcccc tcgaacaagt
    27961 gtccacggtg ttttagtgga tgtttacgga ttaggtgtac tgattcaagg tgacagtggg
    28021 ataggaaaaa gtgaaacagc gttggagttg atcaaacgcg gccatcgatt gatcgcagac
    28081 gatcgagtag atgtttatca gcaagatgaa ttaacggtga tcggagaacc gcctaaaatc
    28141 ctggaacatc tgattgaaat ccgaggaatc gggatcattg atgtaatgaa tctttttggt
    28201 gcgagtgcgg tacgtggctc tatgcaagtc cagttggctg tttatctgga agcatgggca
    28261 aaagataaga aatacgaccg tctgggctct gaagacacga ccgttgaaat cgcggaagtg
    28321 ggcattccac aaataaaaat ccctgtcaaa acaggacgaa acgttgcaat catcattgaa
    28381 gtagccgcga tgaatttccg cgcacgtaca atgggctttg acgcaacgaa aacatttgaa
    28441 gaaagattaa gtcgcttgat tgaagaaaac tcaggcaacg actagcaagg agtgacaata
    28501 tgttagcaca aatcaatcga gttgcttttg agcttgtagg gattcctatc tactggtatg
    28561 cattgatcat cgtatcaggt atcattatcg ctatgtggct tagcagcaga gaggctgttc
    28621 gggtaggatt aaaagcagat gatgtgacag attttatgtt gtggggattg cctatctcca
    28681 ttattggcgc tcgcttatat tatgttttat ttgatcttcc ccagtatata gctgatccaa
    28741 tccaaatatt caatatccgt tcaggaggct tagctattta tggcggactg attgcaggcg
    28801 cgatcacgct gtattttttt actaaatatc attttatttc tttgtggact tttttagata
    28861 tcgctgcacc aagtgtatta ttagcacagg caatcggtcg ttggggaaat ttcatgaacc
    28921 acgaagcata tggaccagac acgacgcgaa gcttcttaga aagtctgcat ttaccgcaat
    28981 tcatcattga taatatgtat atcgaaggga tctatcgtca accaacattt ctttatgaat
    29041 cactttggag tttgattgga tttattttgc ttttgttttt acgaaagaaa ccacaccttt
    29101 tgaaaaaagg cgaagtcctt ttatgttatc taatgtggta tagtttcggg cgtttcttta
    29161 ttgaaggatt acgtacagac agtctttact ttttaggtgt gatccgtatt tctcaattat
    29221 tatctgctgt gttatttgtc ggtacattga ttctatttat ttggcgcaga aaaaaaggaa
    29281 atttaccgga ttatgaccgc tcattaggaa aaaatcaaca aattatctga tttttagagc
    29341 gttaaaaatg aaaaaaattt caggagtggt aaaatgaatt atagagtagc ggttttagga
    29401 ccaggctctt ggggtactgc tttggcacaa gtcctcgccg aaaacggaca tgacgttcgg
    29461 atttggggac atcgtgcttc acaagtagaa gaaatcaaca cccaacacac gaatcaacgt
    29521 tatttaccag atcattatct acccgattcc atccaagcat tcaccaatat ggaagatgca
    29581 gtaaaagacg cagatgcggt gctattcgtc attccaacga aagcaatacg ttctgttgca
    29641 aaagaactag tgcctaaatt gaatacaaag ccagtgatca tccatgcaag taaaggatta
    29701 gaacagggaa cacataaaag gatctctgac atcttgttag aggaaatccc agcagaaaaa
    29761 agaaaagctg ttgttgttct ttctggacca agccatgcag aagaagtagc aaaacatgac
    29821 atcactacga tcacagctgc aagtacagat gcaaaagcag caaaatatgt acaagagctg
    29881 ttcatgaata attattttcg gatctatacc aatcctgatg tgatcggtgt agagatgggg
    29941 gcagctttga aaaatattat tgctattggt gctggcgcta ttcacggctt agggtttggc
    30001 gataatgcaa aggcagcaat catgacgcgc ggattagctg agatcagccg tttaggtgta
    30061 gcaatgggtg ccaacccgct gacctttatc ggattaagcg gaataggcga tctagtcgtt
    30121 acttgcacaa gtgttcattc taggaactgg cgcgcaggca acttgttagg acaagggcag
    30181 cccctagaag aggtgctagc caatatggga atggtcgttg aaggtgtgaa tactacccaa
    30241 gcagcgatgg aactagccca aatgatggaa atcgaaatgc ccatcactca aacaatctat
    30301 gaagtgctgt atgaacaaaa agaaatcaaa acagccgcaa aagaaatcat gttacgtgat
    30361 ggaaaaaaag aaaatgaatt ctcattaaca attttttgaa ggagcgaatg tatatgaaag
    30421 ttaaaaaagc agttattcca gcagcaggat taggaacaag atttttacca gcaacaaaag
    30481 ctatggcaaa ggaaatgttg ccaattgttg ataaaccaac aatccagttc atcgttgaag
    30541 aagcgttgaa atcaggtatc gaagacatct tgatcgttac aggaaaagct aaacgaccaa
    30601 tcgaagatca ctttgattca aatattgaat tagaaacaaa cctaagtgaa aaaggaaaga
    30661 ccgaattatt gaaattagta gaagaaacga cagatgtcaa cttgcatttc atccgtcaat
    30721 ctcatccaaa gggtttagga catgccgttc ttcaagcaag agcctttatt ggaaatgaac
    30781 catttgtcgt tatgctaggc gatgatataa tggaagacga agtaccattg acacagcaat
    30841 taatcgatga ttacgaacgt actcatgctt ctacgatcgc cgttatgcaa gttcctcatg
    30901 atgaaacttc aaaatacgga attatcaatc caggagaagt attggaagat gggttataca
    30961 atgtcaaaaa ctttgttgaa aaaccagatc caagcaaagc accaagtgat ttagctatta
    31021 ttggacgtta cttattgact ccagaaattt ttgatgtatt gtccgagcaa caaccaggag
    31081 ccggaaatga aatccaacta actgacgcaa tcgatacatt gaataaaaca caacgcgtat
    31141 ttgcccgtaa attcacaggt aaacgttacg atgtcggtga caaatttggt tttatgaaaa
    31201 caagtattga atatggtcta acacatccag aagtaggtgc acctttacgt gagtacatcc
    31261 tgtctttagg taaaacatta gaagcagaag gaagcaagat cgatcataat ccaaaaggaa
    31321 taacagaaat caaagaagaa aagaaaaaat aacccttttt tctaacgagc ttcagctgct
    31381 aattgaataa aaagtagttg aagctcgttt ttatttgttt aaaaaacacg aaaatgctat
    31441 ccttatagaa aagagagaca agaagggagt ttgatcatgt caggaggaga aattgcagga
    31501 ttgatcgcag caatcgcctt tgcgatactt gtcgttttta tcgttcgtgt gttgttgcaa
    31561 gtttctaaaa ctttagaaaa agtagatcag actgtagcag aagcaaatac gacgatcgaa
    31621 atcgtcacaa aagacgtaga tcttttgtct agacaagtag aaggcttact ggtcaaaagt
    31681 aatgaactgc tcttggatat caatggaaaa gtcgcaacga tcgatccatt gttcacagca
    31741 gttgctgatt taagtgaaag tgtatcagaa cttaatcatt ctagtaaaaa catcgcgccg
    31801 aaagtcggcg gaatcggtaa aacgacagct aaagcgacgg ttgccggaaa agtcggtggg
    31861 acagcgatga aattttttaa aaacaaaaaa catactgaaa cgacaggagg aaaataaaaa
    31921 tggctaaaaa aggtggattt ttattaggtg cagtagtcgg aggcacagcc gcagcaatcg
    31981 ccgcgttatt gttagcacca aaatctggta aagaacttcg ggatgaatta gctgcccaag
    32041 cggatgattt gatggatatg gcttcagatt acacagatat ggcgaaagaa aaatcgactg
    32101 agttaacagg tattgcaaaa gaaaaagcgc aagatctttc tcaacaagca ggtgatctag
    32161 caggtagtgt caaacaaaaa gtaagtgaat cagcagatga agcaggaagt gaatctgacg
    32221 atatcttgtt aaatcttaaa gaacaatcag ctgacttatc taatcgtttc aaaaaaacgg
    32281 cagaagatat gaaaaaacct gcagatgaat taggaagtat tgctaaagat acttcagaag
    32341 atatcatgat tgatgtaaaa gacgctgctg gtaaagcagg agatactgtc aatgaaggaa
    32401 tgtcagaagc aaaaccagtt gcagatgact caaaagaaat gatgaaaatg gcaaaagaag
    32461 atgcgaaaga agctaaaaaa gaaataaaag atacaatgac agattcatct acaaatcact
    32521 aaaatcacaa atttatagaa taaaaaggtg ggagcggact agctctcacc ttttttactc
    32581 ttatttttct aaaacaagta attcttttgg tgtagtcgtg aatggcacac agccatcgct
    32641 tgtcacatat acacagtctt cgatacgtac ccctactttt tcaggaatat agattcctgg
    32701 ttcaagagag aagcacatcc cttcttcgat tactaagtca tttccttcta caagggaagg
    32761 gaattcgtgg acggttgttc cgatcccgtg acctaaacgg tgattgaaat attcgccgta
    32821 gccatagctg ttgatcacgt ttcgtgcgat ttgatcaagt tcacctgctg tcacacctgg
    32881 acggacggct tcttgtgcgg caagctgtgc ttcaagagtg atgttgtaga ttttttcttg
    32941 gaagtcggtt ggtttttgat aagcaattgt acgagtagca tcactgcaat agccattcca
    33001 aacaacgcca agatcgaata acacaagttc attcggtgaa atcttcgtat ttcctggtgt
    33061 accatgaggg cttgcagcat ttgctcctgc aaggactaat gtgtcgaaag acatttgtga
    33121 aacaccttgt tttttcaatt ggtattcaat ttctgcaatg atcgcttgtt cagtcacgcc
    33181 ttctttcact gctttaaagc caatctcaaa tgcaacatcg gcccatttac cagcttcgat
    33241 cagtttgtct ttttcctcta gagttttgat cagttgtaga cgttggatga tggaagttag
    33301 atcagtgtaa gaatccgttc ccgtcaagat atcagagata gcatcgaagc gctcgacagt
    33361 caaggcgttc ttttcaatcc ctacattgcc aggctgacca tagcgagcac gaagttcttt
    33421 tccgatgatc tcccaagggt tttcgctgtc taaatagcca cggacatcat acgcccaact
    33481 actgttttga gcatcttcaa cttccaaagc aggagtgaaa aggaaaggat cttgttcaag
    33541 agggataaaa agagctagta cacgctcatg aggatcactt tcgtatccgg aaaagtaagc
    33601 gatgtggcca gggtcgctga tataagccaa ttcgacttgg tgttgtgcca tccattgacg
    33661 tagttcattg atttttacgt tattcatttg gcaacctact ttctaaatta attttgtatt
    33721 caataattgt accactgatt tattaaaaga ccaacgttct aaaaactgaa aaaagtaaaa
    33781 attttcatga aaacagagat aaaacggttg caattcttga gattatttga tattctattt
    33841 tagaaatgta agacgtattt tcagaaaaca tataaaatat gaataggaga acacaaatgg
    33901 aaaagcaaac aatcactatc tatgatgtag cgagagaggc aaatgtttcg atggctactg
    33961 tttcacgtgt cgtaaatggc aatccaaacg ttaaaccagc aacaagaaaa aaagtcttgg
    34021 aagttatcga tcgcttggat tatcgtccaa acgcagttgc gcgtggcttg gcaagtaaga
    34081 aaacaacaac agttggtgta atcatccctg atgtcagcaa tatgttcttt gcttctttag
    34141 cacgtggtat cgatgatgtg gcaacaatgt acaaatacaa tattatctta gcaaattcag
    34201 atggcaacga ccaaaaagaa gtcaatgtat tgaataactt actggctaag caagtggacg
    34261 gcgtgatctt catgggtcac catatcacag acgaaatccg tggagaattt tcacgttcaa
    34321 aaactcctgt tgtattagct ggctctattg atccagatga acaagtaggt agcgtaaaca
    34381 ttgattatgt atccgcaaca aaagatgcag taaacaaatt ggcaaaaagc ggcaataaaa
    34441 aaatcgcttt tgtcagtggt gcgttgatcg atcccatcaa tggacaaaat cgtctgaaag
    34501 gctataaaga agcattaagc gaaaataacc taccatatag cgaaggattg atctttgaag
    34561 cacaatacaa cttcaaagat ggtctatctc tagtcgatcg gatccataat agcggtgcaa
    34621 cagctgtcta tgtttcagat gatgaattag ccatcggtat tttggatggt ttattagacc
    34681 gtggcgtgaa agtaccggaa gaattcgaaa tcatcacaag taacaattca ctactgacag
    34741 aagtagctcg tcctcgttta acaagtatca cacaaccatt atacgattta ggggcggtat
    34801 ctatgcgcct attgacgaaa atgatgaata aagaagaagt cgaagaaaaa acgattatct
    34861 tgccttatgg aatcgaagaa aaaggatcta caaaataata aaaggacaac cattcagagg
    34921 ttgtgtgcct gccgtttaac tctgcgttac aaggagcaat ttcaaaaaac agcatctcat
    34981 gtttgatgaa tattgtgact cgtgattcgg agttggcagg agaacaccgt ttatttggga
    35041 aaaggggtta tgacaaaatt ttgttataat cctttttttg tgtcttaaaa aagagagaaa
    35101 gtggttgaaa ttacatttat cgtatataaa acaatccgtg tatagatgct gcattttgtt
    35161 caccaccatc aaaaaaataa gggactgtga caatactatt gtcacaatcc cttattactg
    35221 tgtattctat taaatcaatc atctttctca tctgattctt tagttgcttg attcgctctt
    35281 gcataagctg aagcggtgtt ccagtaatct gtatagtttt catcggtacc gccaacgcca
    35341 aatttaaagg cgcttttctt cggtccattt ttcgcccata gcgattcaac tgtttttcct
    35401 ggtgtttgga tggatttttt attgacagta acttttccag gtgtttgacc agtaaatcca
    35461 gatacttttt tcttcatcac atcagatgat aattcgaatt tctcatcagt gccaaaaatg
    35521 tcaggttttg cttggtaagc ttgattgatc agattggcaa gataattagc cgaacgtatc
    35581 cttgctttgc tgtccattcc agtattgtca tcgtgccctg cccagctact gatggtaata
    35641 gacggagtag agacaattag ccatgaatct cggtattcat tggtagaacc agtttttccg
    35701 acccaatctg cttttcctag atttccgttt aagcttgaga tggcatcttt gaatggtgta
    35761 gtgatttttg cattgatcac actacgcatc atgtcattca tgatcgatgc cgttgcttcc
    35821 gaatagatcc ggacagagtt gctttcgtgt ttataaatta catttcctgc gttatctgtg
    35881 atggaatcga tgatatagcc ttcttcatag acgccgccgt ttgctaaagc ctgaaatccg
    35941 ttggtctgtt gaagcgtcgt gacatctact gtacctaaag gagcagattc aacgccccaa
    36001 ttgtcatttg ccgggtagtt catctttgcc aagtagtgct cgtaagcata ttgtttcgat
    36061 ccaccggaat tcaacacatc ttgatataag tgataagccg ggatattgtt ggaccattct
    36121 agtgactcac ggacggtttg gaaggtgttc gatccttcgt tagtagcatt gacgattttc
    36181 tcgccagcat tttctccttc ttgccaagtt gttgcataat ctgatacacg ggattctgat
    36241 ccgataagcc ccatgtcgat tgcaggtcca taaacaagaa ctggtttgat tgatgagcca
    36301 gcttgacgtt ctgtatcgaa ggcatggtta ttttgattct gactgtaatt ccgtccgcca
    36361 acgaatccgt agatgcggcc agttctgtta tccatcaata catttccggt ttctacttga
    36421 ctgccagcac catcatctaa catgtatcca taattttcaa cgccattttg caatgcagca
    36481 tagacatctt tatcgattgt cgaatggatt gtatatcctt tattttggat cgtttgctgt
    36541 gccttttgat aataagcgtc actgaccgat gaatcagaca tatcagcgtt gtctttttca
    36601 gccaattgtt gtgcaatgat tcttgttgct tcattcatca cagtataata caagaattct
    36661 ctgtcagtct gttctgcgat ttgttgtggt agaaagtctt ttgtaaggtc ataggctttt
    36721 gcttcttcgt attgttcttt cgtaatttgg ccttcacgat acatactgaa taaaacaaaa
    36781 tcttttctgg caaggccggc agaaagatct tcttttagcg cgcctgtgtt cgtgtatgga
    36841 ctatacgtga tcgggctttg gggtaatcca gcgatataag cagcttgcgg cagcgtcaca
    36901 tcttttgcat ctacaccaaa gattcctttt gcagcttcct ggactccagc aatgttttgt
    36961 cccttattat tacgaccaaa tggtgagaca ttcaaatacg tagcaatgat ctcatctttt
    37021 gaaaagtatt tttctacttg tgcagaaaga atgatctcgt ttgctttacg tttgaatgtt
    37081 gtttcatctg taaggatctg ctgtttgact aattgctgtg tcaaagttga tccaccagaa
    37141 gagccaatac cagtagcttc agatactaat gctcgtaaaa cagctttagg aacgtaacct
    37201 tggtgtttat cgaagtattc atcctctgtg ctgataattg cagttttcaa taaaggtgaa
    37261 atcttatcag atgaaatagt cgtgcgcatc agatctgtct ggatttttga tatctctgta
    37321 ttatctgcat aaacaatctt tgacgtttct gtaatgtttc ctagttctgt ctgcagtgct
    37381 tttttagtcg gcaattgtgt gtcttcgacg agataggcaa aatagccagc gcctattcca
    37441 atacctagag ctgcgaacat caaaagaaga acagtaataa aaacagtcaa tgattgaaaa
    37501 actctaaaaa taatcttagg aacaaaccat ttttcttttt ttcttctatt ctgttttttt
    37561 cgagtatttt tgcgtggcaa aagggtcacc tcaactaatg gttttatttc gctaattata
    37621 gcaaataata gaacaaaaaa acagacaagg ctgtttttgg caggaatttc ttttttaaaa
    37681 gagttaaaac gtgagaaaat gatctttatt ataaaagagt tcttaagctt ttctttagag
    37741 aaaatttcag ttactatgga gaacagggca aagaaaaatc aatcattttc tttgtatatc
    37801 atcttaccaa tatataggaa ttgttagtaa caattgcctt tcaaggagga atccatccat
    37861 gttattaggc tcacacgtca gcatgagcgg aaagaaaatg ctgttaggag cagcagaaga
    37921 agcagccagt tatggatctt cgacttttat gatctataca ggcgcccctc aaaacactag
    37981 aagaaaagcc atcgaagaga tgaatattga tgccggaaaa gcgtacatgg cagaaaaagg
    38041 tctaacaaat atcgtcgttc atgcccctta tatcattaat ttggggaata cgatcaaacc
    38101 agaaatgttt ccttttgccg tccaatttct gagagaagag attgttcgtg cggaagcttt
    38161 aggcgctact caaattacac ttcacccagg tgctcatgta ggggcaggaa cagaagccgg
    38221 aattgctaga attattgaag gcttgaatga agttttgaca aaagaacaga cggcacaaat
    38281 cgctttggag acaatggcag gtaaaggtac ggaaatcggt cgaacatttg aggaattagc
    38341 gcaaatcatc gatggagtaa aattgaatga aaaattatcc gttacttttg acacctgtca
    38401 tacgaatgat gccggttata atattaaaga agacttttca ggtgtaatgg aagagtttga
    38461 taagatcatc gggttagatc ggcttaaagt actccatatc aatgattcta aaaatccgca
    38521 aggttcgcat aaagaccggc acgccaatat tggttttgga acaatcggat tcgacacgct
    38581 gaataagatc gtccatgatc caatgtttga caatatttcg aaaatcttgg aaactcccta
    38641 tgtaggcaaa gataaaaaga aagcttacgc accatatgga aaagaaatcg ccatgttcaa
    38701 agcgcaaaca ttcgatccag aagtgtttcc tgagttatta gcagaagaag tgatactcta
    38761 agattttcta tctgacagca gcaaaaaagg accaggcaca ttgccttgtc cttttttgtt
    38821 taagaaacaa aggagtactt tttgcagtct ttttcatcat gcatcttctc ccccctcttt
    38881 ttaaatttag acaaacaagg tatactggta gaaaagtaaa aaaacgaaaa gtttcacaag
    38941 caagtggcaa aacgtgattg gaaggacgaa aagatgctga acactttaaa acccttgatc
    39001 aaccaactaa aaaagaaaca tacatacttt attcttggca tagccctatt atttacaatt
    39061 attcgtttgt atttatatag taaagcaatc tatggaacag acatgtcggc aacctatgat
    39121 gaccagctgc tgattcgcta tgctcaaaat attttggacg gtaaatggct aggggagtat
    39181 acgactacaa cactttccaa aggaatctcc tacagcttat ttttagtact tgcacataaa
    39241 ctgcatcttt cttatgcagt tttgttcggc atgttcagta tttttgccag tatattggtt
    39301 gcactcgcgt ttaaaccgat tgtgaagaac aagtatcttc ttttaagtat ctatttttat
    39361 tttttgtttt ctccgattag ttttacacat gaatattcca ctcgaacata tcgtaatgcc
    39421 ctcgtgattc cggctgttga gatcattatc gcttgtttat tagctattta ttaccgcaaa
    39481 tacggttcta gcagaaaatt gctgccatgg tttttagggc tttctgtcgc ttttcctttc
    39541 ttttggttca ttcgcgaaga ttctatctgg cttcttcctt ttactgtagt agctttgggt
    39601 atcagtttcg tccagatctt gctagggcaa gacttttctt tcaaagaagt aaagaagtta
    39661 aaaaaacgac tctttcttct ctcgaaacgg gaatggctga aactttcatt gtttttactt
    39721 cctatagttg gcttttccat cgtctatcaa tggatcgaat ataaaaatga gacaacatac
    39781 ggtattgcaa ccatcaatga ccgttccagt ggagaatttg gacgtttgat gaaacaactg
    39841 atccgaatag atgatgaaac gaacttgaat aaagaaaaca gtgagatatg ggtctcacat
    39901 gctgcactaa ataaagcatt agccgcatct cctacattcc atactgtttc tacccgtatt
    39961 gacgaattat atacgactca cgcgtggact caaggtggaa aagtaaaaga attacccgga
    40021 gatatcatct tttgggcatt gcgagatgtg gtaagcgaat ctggttatta ccaaaacaat
    40081 gcaaaagaaa ccaatgattt ttggaaaaaa gtcaaccaag aattagaaga agcctatcac
    40141 aagggaacaa tcaaaaagaa aaaagagttc tatctgatgg gatcaggaga tggaaagacg
    40201 atgagtgatc tgccaatcgt attcgatttc cttcaatcag gatacgctta tgcatggcga
    40261 tataaagatt tccatcaagg aggaaattat agtttcggaa cgcaagaaca agtcaaacag
    40321 gctcaagaat tattaaacct tccgctaatg gagaattgga tcgatagtaa tgaaccgaga
    40381 acacctaatt tccaattaac aaaatcagca aaagtcgcga atgtcatcat aaaaatttac
    40441 caagttctgg ctattcccgt gctgattgct tcaatagcag ggatattact tatgatacta
    40501 ggaaccgtat ttgcaaaagc tgatcgtgcc tattatagtt cctctttgat tgtggtcacc
    40561 ggattaatac tgacacaatt ggtttttctt ttcggtgtct catggttttg ttcttatgcg
    40621 ccagaaaaaa aggactattt tttaagtgtc tacactggtg caggcgtgcc attaacgcaa
    40681 ttcatcgtag taacaagcat gctagttttc agtaaattaa atctgaaaaa agctttatct
    40741 tctaaataaa aagacccttg gtagctagat ttttaaccta actatcaagg gtctttgtgt
    40801 ggaaaaatca gtttttattc gatttcttct accgtttcct gcaaaagctg ttcatatttt
    40861 ttgtttcctt tatataaatc accaatcgtc catgccaaca ggagaaggac aagaacaatg
    40921 attacataag cgatgtgatc agccaatgct agttgagcgg aagtggcttg ctcgccgaag
    40981 aaactttcga tctgtcctgg taatcctcgt aagttcaagt aagttagact aatcacagag
    41041 aaccagccaa gaagttttac gatgaatgaa tttttaaatc ggctacccat ctccgcttgg
    41101 ctgttggtca tcatcagtaa aggaatcatc gaaaacggca acgcaaaagc caagaacacc
    41161 tgggattcat tcattaagtt attcaaagcg gcatgttctt cgatcgttcc ttttgtactt
    41221 gtaaacaaaa cacaaatcaa tacaggaatc acagaaagca atcgtgtgac cagtcttctt
    41281 agccaaatcg ggatacgcat atggatgaat ccttccatga tgacttgacc ggtcaacgta
    41341 ccagtaatag tagagttctg gccagaagca agaagtgcta cagcaaataa agtggacaag
    41401 ataccagatt ttgctacagc aatcaaaata ccattgctca tagctgatgg atcagataaa
    41461 gcttcataca gaccaaaaaa ggaaggatct tggacagaac cggttttgaa gacagcgaca
    41521 cccatgatca atagcagtgc attgactaga aaagccattg acaactggat attagaatcc
    41581 caagtagaaa aacgtacagc ttgtgctaca tcttcttcgt tttggtgatc gatcttccga
    41641 gtttgtgaaa ctgctgaatg aagatataag ttatgcggca tgactgttgc accaataatc
    41701 cctaaagctc cagtcagcgg agtttcgccg ccgatgaccg gtgagtctga gaatgtatga
    41761 ctgttaggga taaagccagc aaataaactg tgccaatctg ggttagacaa gccaacttga
    41821 tagacaaaaa cgaataaaat gacgaaaatt aagcagacaa ccagtgcttc gatcttacga
    41881 aacccgactt ttgtcagtag taagagaacg aaaacatcta atacagtaat aaagacggca
    41941 atcaccaatg gaatatcaaa aagtaagtag agggcgattg ctgcaccaat gacttctgca
    42001 atatctgttg ccataatggc taattcagtc aagatccata aaatgacacc taatgtctta
    42061 cttgtgcgtg ctcggattgc ctgtgccaaa tccatttggc tcacgattcc taattttgct
    42121 gccatatact gtaaaagcat agcaatcaga ctagacatca aaataactga catcaataaa
    42181 tattggaaat tttgtcctcc ggtgatcgaa gtagaccagt tacccgggtc catataccca
    42241 actgctacta acgcaccagg tccagagtaa gcaagaagtg ttttaaaaaa acttttcccc
    42301 tttggtacat caactgttcc atttacttca gctaaagata aaccatttgt atgttcaata
    42361 agtcgatgct tatgaaattg tttattggat tgattattca agaaaaaacc acctttccta
    42421 tttatctacg attacattct acacctcaaa aagaaaaaat taaagaaaaa gattcggtac
    42481 gcctaatttt ttttcatggc gcgtcttgct agatgacgaa agaaattttg ttctcctcta
    42541 aaaaaaccac tattttttgc atgaaactaa agaatacgcc tttctttttt caataaactt
    42601 tagtaaaata agtcattgag agcaattcgg aaatagaaag gagagattta tatgttatca
    42661 acaggtattg tcgtacttat tgctattatt gctttgctaa tcggcgcagt aggtggcttt
    42721 ttcctagcgc gtaaatatat gaaagactat ttagaaaaaa atcctcccgt taatgaggac
    42781 atgttgcgat caatgatgat gtcaatgggg caaaaaccat ctgagaagaa aattcgccag
    42841 atgatgcagc aaatgaagaa ccaagggaaa aaataggttc tgacccaacg actttttacg
    42901 ggctgatttt ttcagtccgt tttttatttc gtaatttgtt agactattca gattattcag
    42961 caaaaaagac aagcacagac aatcctaggc tcttgataaa gacaaaggag gagtaaaatg
    43021 tcgattttta gaaaattagg ctggtttttt cgtcaggaaa agaaacatta tttaattggg
    43081 gttttctcgc tattcgcagt ggccttggta cagttagttc ctccaaaagt gattgggatc
    43141 atcattgatg agatcgccga taaaaattta tcaataaaga tcattttcgg gtggatcttc
    43201 ctattgatta tcgctgcttt tttacaatat ctttttcgtt atatctggcg gacgaatatt
    43261 tggggaagtg cagctcgatt ggagaaagaa cttcggcagc agttattcaa acattttaca
    43321 gaaatggaca atctctttta ccaaaaatat cggactgggg atttgatggc tcacgcaacg
    43381 aatgatttga atgcgatcca gaatgtagca ggagctggta tcttgacctt tgcagattca
    43441 gtgattacgg gcggaacaac gatcattgcg atgattttgt ttattgattg gcgcttaaca
    43501 ctgattgcgc tgattcctct gcctttgctg gccgtcactt cacgagtgct ggggtcaaaa
    43561 ctccatgatg cttttcgtga ttcgcaagaa gctttttcta gtatcaatga taagacacaa
    43621 gaaagtatca ctgggatcaa agtcattaaa acatttggac aagaaaaaga agatctagcg
    43681 gattttgaag aaaagattga agatgcaatt attaaaaaca aacgagtcaa ttttttagac
    43741 tccttatttg atccattcat taccttgatt atcggtctgt cttatgttgc aacaattatt
    43801 ttaggtggaa agtatgtctt ggaaggcacg atctcgatcg ggcagatggt ctcatttatt
    43861 agttatatcg gtatgcttgt ttggccgatg tttgcgattg gtcgattgtt caacgtattg
    43921 gaaagaggaa atgcttctta tgatcgcgtc aatgaacttt tgcatgaaaa gacgcatatc
    43981 gtggaacaac tgggtgcagt gcagaccccg gcaaaaggcg agctgaatgt agctatttcc
    44041 tcttttaatt atccgaaaga agaatcacca gcattagaac aaatcaagtt tgcgatccaa
    44101 gaaggagaaa cattaggtat tgttgggaaa actggtgcgg gaaaaacgac gatcatcaaa
    44161 ttactgatgc gtgaatacga tgattatcaa gggaaaatcc aatttggcgg acgttcgatc
    44221 aaagaatatt cattgaatgc tttattagga tcaatcggct atgtgcctca ggatcacttc
    44281 ttgttttcga tgaccattcg agataatatc cgtttttcta atccaaaact ttcagaagaa
    44341 gaagtagagc atgcggcttc tttagcattt atcaacaatg aaatcaagga attaccaaaa
    44401 ggttatgata ccttagttgg agaacgtgga gtctctttat ctggcggaca gaaacaaagg
    44461 atttcgattg ctcgagcatt gatcaaaaat cctgaattat taatattaga tgatgcactc
    44521 tctgcagtag atgccaaaac agaagaagct atcttgcata atttaaaaga aataagaaaa
    44581 gagaaaacaa ctatcattgc agctcaccgt ttaagtagcg tgatgcatgc aaaagaaatc
    44641 attgttgtcg atcaagggaa aatcattgaa cgaggaaccc atcatgagct gcttgcacta
    44701 ggcggctggt atgaacaaat gtgggagaaa cagcaattag aagcgaagat agaaggaggc
    44761 gaggcctgat ggatgaaaat tatcaatcgg aatggacaaa atcgattcca cttaaagaac
    44821 agaaaaagat catcaaacga ctgatgaagt tcgccaaacc ttttagaaga acgttcatcg
    44881 ttgctatctt atttgcgttt gctttgtctg tgatcaatgt cctattgcca aggatcatcc
    44941 aaacttttat ggatgatcat ctggctaaac agtcggctac gacacaagtt attttgtttt
    45001 ttgctggtat gtacttattt ggagtcatag taaaaagtat catttggttt ttccaatggt
    45061 atttgtattc catggcctca ttgaagacct atcagtatat tagggtaaaa ttatttgaaa
    45121 agctgcatac actaggtatg cgttattttg atcagacacc ggcaggatca accgtttctc
    45181 gagtgacgaa tgacacagaa acgctatttg aattttggta cgtctttttg atggtattga
    45241 ctgggatctt tgcagtcgtt tcttcattcc ttgccatgtt tcaaatcaat ggagaaattg
    45301 ccttgtggac tttggtgttt ttgccgattt taggaatcgt catctggtat taccagaaat
    45361 tcagttctcg tatttaccga aatatgcggg aaaaattaag tcagctgaac acgaaattga
    45421 atgaatcgat ttccggcatg cgtattatcc agcagtttcg ccaagaagct cgtttagcta
    45481 aagaatttga acaagtgaac aatgagtatt tagacacgcg ttatgcgatg atcaaaacga
    45541 attcgattct gttaagtccg atcatcaatt ttctatatgc attggcgatt gcgttggtgt
    45601 tgactttgtt tgggatcgat gcgttgcatt ctcccgtaga agttggactg atctatgcat
    45661 ttacgaccta tgtccaggga ttttttaatc caatgtctca aatgatggac tttttgagca
    45721 tcttcacaga tgggatggtt gctggtagtc gtatcttgaa gattatggat acggaagaat
    45781 atgcaccaca acaaaaccct ggagcagatc aaatgattac ttcaggtaag attgagtttc
    45841 gcaatgtaag cttttcttat gatggagagc acaatgtatt aaacaatatc tcttttgttg
    45901 ctaatccggg agagaccgtt gcattagtgg gacacaccgg aagcggaaaa agctcgatca
    45961 tcaacgtatt gatgcggttt tacgaatttt atgaaggtca gatattgatt gatgatcatg
    46021 acattcgaga atatccaatg gaagagttac gtaagaaaat ggggctggtc ttgcaagatg
    46081 ctttcttgtt ttatggggat atcgcaggaa atattcggat gatgaatcca gctatcacag
    46141 acgaacagat acgccatgcg gctgagtttg ttcaagcaaa caaattcatc gatgaattac
    46201 caaaaggata tcactcaaaa gtgatcgaac atggtgccag ctattctagt ggacaacgac
    46261 agctgatcac ttttgcgcga acaatcgtga ctgatcctaa aattctagta ttggatgaag
    46321 caacagcaaa tatcgatact gaaacagaag gattgatcca agaaggattg gcaaaaatgc
    46381 gtcaaggacg gacaacgatt gcgattgccc atcggctgtc aacgattcga gatgcagaat
    46441 tgattttagt tttagacaaa ggtcatatag tagaacgcgg gaatcatgaa acacttctag
    46501 cgcaaaatgg tttgtatgca gatatgtaca aactgcaaag cagtggtata taaaagatgt
    46561 tgtgacagta gaattttgac ctaagcacta gagtgaaaat aaaacatagc ttctcatatt
    46621 tttcatctgt gagagttagt gtcgtagtca ccagatttaa aaaaagattc gacagctagt
    46681 cagaagtgcc taatagccac taagaaagcg ttgacaaaag catttgcttt cgataatata
    46741 aagaaaacga tacaacttgg atagtgattg cctgatgcaa gctaaacctg atgagtctcc
    46801 catggtttca tggacggctt tttgggtttt tctttttgtt ttggatacag ccctatacat
    46861 catggaaagg agcaaaacat gctgattgag aaagtactga ataacaacgt cgtaataacc
    46921 agggatgaaa gaaatcagga aatgatcgtg atgggaaaag gtttggcatt caaaaagaaa
    46981 gtgggtgacc cgatttttga ctcatgcatc gacaaaaaat ttcagttgac aaatgacggg
    47041 ctttcacaac agttccaaga attggcacaa gcgattcctc ttgagtactt agaaatttct
    47101 tgtgaaatca ttgaatatac aaaagaggct ctacaagtcc agttgaatga ttctgtgtat
    47161 attgcattga cggatcattt gtatacggct atcgaacgtc acaaaatggg aatatttgta
    47221 ccaaatgtat tgctgtatga tatcaaacaa ctatttccta aagagtatgc catcgggaaa
    47281 aaaacagtga agaaaatctc ggataaatac cacatcaagt tatcagaaga tgaagccgga
    47341 tttatcgcat tgcatttagt caatgcacaa ggcattcaaa gccagcaaga caatacctac
    47401 ctgtttacta taacaataca ggatattcta aatatcgtcc gctattacta taagattaca
    47461 tttgatgaag attcagtcca tttttttcga ttcgtcacac acttacgttt ttttctgaca
    47521 cgcgtcctag atggagaaaa atcagaagtg ggagaagttt ctgaggaatt gtttcaggtt
    47581 attcaaaaaa aatataagga tacgtttggt tgcgttgaga aaatagcaga ctttttagcc
    47641 gagacgttca actaccagat gtccacggac gaaaaaatgt atttaacgat ccatactgcg
    47701 cgtttagtgc atgagaatcg ttgtaaataa aaaaaggagg ataaattaac aagaacgtat
    47761 gaaataaggg tagtgacatt cagttggcta aaccttccac taataaattg gagggaatag
    47821 aaatgggaaa ataccaagaa ttagcgagaa aaatcgtaga aaatgtcggc gggaaggaaa
    47881 atatcaatgg tttgacacat tgtatcacaa ggcttcgttt caaattaaaa aacgaagaaa
    47941 aagcaaatga tgagatcttg aaaaatatgg acggtattgt aacggtaatg cgagcgggag
    48001 ggcagtatca agttgtcatt gggaatcatg tgccagtcgt gtttgaagag gttataaaag
    48061 caggaaacct tacttttgat gaggctgttt caacagaaaa aatgagaccg tttgatatgc
    48121 ttattgatgg aatatcgggc tgtttccagc cattccttgc tatcctagct gctggcggga
    48181 tgattcgtgg actgacagca ttccttgtct tcttaggcgc gtttgaccgc ggttctggca
    48241 catttgttat gtttgataat atcggtgatt ctgtattcca atttatgcct gttattatcg
    48301 gtttaacagc tgcccgtaaa tttaaagtaa atgaattcgt agggatgttg cttggcgcag
    48361 cattaatgaa tcctagcttg tcgctggaag ctttgagtgg tgcagcagaa gcaccactaa
    48421 cgaccatatt ttctggcact atttttgaag cacctattta tcaaacagta ttcggcattc
    48481 cctggattgc gcgtaactac gcttcttctg tcattcccat tatctttatt gtgttgttag
    48541 ctagccaaat tcagaaacca attaaaaagt tagtacctga gatgatcgcg aatttcttcg
    48601 ttccattctt caccgttctt attacaatgc ctttaggttt tttactcgtt ggaccagtat
    48661 tcacattcgc aactgatatt ttaatggctg gatttgaaac acttttagca ctttcgccag
    48721 ttatttatgg agcaatcgta ggcttcttct ggcaaattct ggtgatgttt ggtctccatt
    48781 gggcaatcgt accaatggga ttgatgcaat tttctgtgaa tggttggcaa aatattatga
    48841 cacctgttgc agtagtgtct tttggacaaa ctgcagcact taccgcactc tactttaagc
    48901 ttagaaatcc aaaagacaaa gcaattgcaa taccagccat tgtttctggt attgtgggga
    48961 ttacagagcc agctatttat gggtttacat taccacgtaa aaaaatattt atttttacct
    49021 gtgtcggtgg tgcgttaggg ggcgcgtata gtgggttaat gaatttaact tcttggaatc
    49081 aaggtggact aggcatcttt acaattccca actatattag accagatggt gatttaacag
    49141 atgtgattaa tgtattgatt gggatcgcca ttgcgatggt ggtatccttt acgctgactt
    49201 ttttcttttg gaaggatgaa gcaggtgaaa cagatgatat acagaaaaaa tcaggtaaag
    49261 aaattgtaaa aacaccgata caaggacaaa tcgctccact aaatgcagca aaggatgcag
    49321 cttttgcaca aggaacacta ggtcgaggaa tcttgatcta ccctgaaaaa ggagaagtac
    49381 gtgccccttt tgacggaaca gtcatgaccc tgttcccgac gaaacaggca attggaatgg
    49441 tttctgaaac agggctggaa ttgctgattc atgtcggatt ggatactgtg cagttggaag
    49501 ggaaatattt tgaaagctta gtgcagcaag gataaagagt gaaacaaggc gatctgttgc
    49561 tccgcttcga caaaaagaaa atcgaaaagg aaggatacag tttagaaaca cctgtaatcg
    49621 tcaccaatta catggattat ttggatattt tggaaaatca aggggagacg gtcgggccag
    49681 acgatgcact tatcaccgca ctgacctgaa cggttaggtg aaagaatgga atagacttaa
    49741 ctattctttt tagtccactt ctttattttc aaaattgaaa ggctctttca tgatggctga
    49801 gctgataggg cgtttatcaa atttttgtcc tacagaaatt agatggacta aattctgttg
    49861 ataattttct gcaaaaacac aactatataa cacgtctaga agataacaaa tcgatgtatt
    49921 gatcgtaaaa ttaccaattt ttgaatacag tcgttcgcgt gtcgtaagtc gcaaagcggc
    49981 tgtactcatt gaggatagcg ttgaatcacc gatactggtc aacgctaaaa ttggaacatg
    50041 attttgtttc aatatttctg cggtacgaag gatcacattg ttttcacccg tataagaaat
    50101 cagaatcccg cacatatcac tcgtgcaatt gtacgcttca tagaacgatt caccaaaagt
    50161 ggtgacaatc gtgacatcca ttttgatgcg cttcattttc agcatgaaat cttgagaaat
    50221 caaagtattc gagttactgg cgaaaatcac gatttttttt gcttgaagca ttagttgttt
    50281 ggctttttgc agttggtcat gatgaatgag agaaagtgta tcttgaatcg tcatctgttc
    50341 taaagaagcc attttatttg cgatcgtcat aataccgtct gtggtggaaa aaggaagatt
    50401 cgcatctacg tcttgaaaat tcccttcaag ataggctgtt tcagctaaaa aagcattacg
    50461 aaaactgacc cagccatcaa aacccatttt ttttgctaca cggatgagtg tggacgggtg
    50521 gacataacat cgctcagcaa tctcttggat ggtcatttcg ccaagtgctg gagaatcgct
    50581 taagagaaac tgaatcactt cattttctga aggagaaaag gagatttctt tgattttttc
    50641 acgtaataac atgtagatac cttcttctag tttttttacg taaattataa cttttttgaa
    50701 aatgatttgt aaacggattt atgttatata gcttaataaa gtcatttttt gacaagatat
    50761 ttacaaaaat ccagtagaaa acactaactt ttgtaaatgt tactggaaag aaacgaattt
    50821 ctattgtttg ttttgtttac aaaaaagata ataaaagtgt aaaaagcaaa ggagtgagaa
    50881 catggcaaaa ggaatcaaaa tcgctacgat tggtggaggc agcagttaca cccccgagtt
    50941 gatggaagga tttatcaaaa gatatgagga attaccgata cgagaaatct ggctcgtaga
    51001 tgtagaagaa ggaaaagaaa agcttgagat cgtcagtcag atgtctcagc gtatgtggga
    51061 tgcttctccg tatgaagtca agatctatgc aactttggat cgggaagaag cattgaaaga
    51121 tgcggatttt gttacgactc agtttcgtgt cggtcaatta gatgcacgaa tcaaagacga
    51181 gcggatccct agttattatg ggatgattgg gcaagaaaca aatggtgctg gcgggatatt
    51241 caaagctttt cgtaccgttc cagtggtttt ggcaatcgtt gaagacatga agcgtttatg
    51301 tccaaatgct tggctgatca atttcgcgaa cccaagcggg atggtgacag aagcagttat
    51361 cagatatggc aaatgggata aagtgatcgg attatgcaat gtgccagtaa tggcgacgat
    51421 gacagaacca gcaatgcttg gcaagaaaaa agaagagctg atctacaagt ttgccggtct
    51481 aaatcacttc cattggcaca aagtagcaga taataaagga aatgaccgca cagcagattt
    51541 gattgatcgt ttatacgatg aagacaatgg attgccgaaa aatatctttg acatcccgtt
    51601 tttcaaagaa caattaaaac aaatgaacat gatcccttgt ggctaccacc gctattacta
    51661 ccgtgaagaa gaaatgttgg aacatgcttt agaagaatat caaacgattg gtacaagagc
    51721 gcaacaagtg aaagaaacag aagcagagct gtttgaatta tacaaagatc cagaattgga
    51781 tcataaacct gaacaattgc aaaaacgagg tggtgcttat tattcagatg ctgcctgtga
    51841 aacgattgct tcgatctatg gaaataaaga aacacagatc gtggtgtcta caaagaatga
    51901 tggagctgtt cctgatctgc cggcagattg tgtagtagaa gtcactgcct atgttggcgc
    51961 acaaggcgca cgaaatgtag cgtttggttc ccttcctgct gctgaaagag gctggttgca
    52021 agtaatgaag aatatggaat tgctgacgat cgaagcagcc gtgactggtg attatggaac
    52081 agctttacaa gcgtttacga tcaatccgct tatacgatca ggaaagacag caaaacggat
    52141 catggatgag ttgtttattg cacatcagca gtatttaccg caattcaagg aaacaatcag
    52201 ccggttaaaa gcagaaggaa tcgaagtcca agatgaactg gcaagagaat tgtcaagcaa
    52261 tacacatgta atggaggaag tatgatggga ttcagaaaag attttttatg gggcggtgct
    52321 acagcagcca atcaatgcga gggtggttat gatgaaggcg gacgtgggtt agcgaacgta
    52381 gatgttgtgc caatcggaga agaccgtttt cctgtcatta ctggaaaaaa gaaaatgttt
    52441 gaatttgata aagatcattt ttatccagca aaaattgcga tcgatatgta tcatcattat
    52501 aaggaagata ttgctttgtt tgcagaaatg gggttcaaga cttatcggat gtccattgct
    52561 tggagtcgta tttttccaat gggcgatgaa aatgagccaa atgaatctgg tttgaaattt
    52621 tatgaggatg tctttaaaga atgtcaaaag tacgggatcg agccgcttgt gacgattaca
    52681 cattttgatt gtccgatgca tttgatcaaa gaatatggtg cgtggcgttc tagagaactc
    52741 gttggttttt atgaaaatct gtgccgagtc atcttcaatc gctataaagg acttgtcaaa
    52801 tattggttga cattcaatga gatcaatatg atccttcatg caccattcat gggcgcaggg
    52861 ctgtattttg aagaaggaga aaatgaagaa caaatcaaat atcaagcggc acatcatgag
    52921 ttggttgcaa gtgccatcgc aacgaagatt gctcatgaag tagatccgga gaacaaagta
    52981 ggctgtatgc tggcagcagg agcatattat ccatacactt gcaatcctga agatgtttgg
    53041 gcttctagaa aatcagatcg agaaaattat ttcttcattg atgtccagtc acgaggagaa
    53101 tatcccgctt atgcattgaa agaaatggaa cgaaatggca ttcagatcaa aatggaacct
    53161 ggggatgccg cattgctgaa agaacatact gttgatttta tctccttttc ttattattct
    53221 tctcgagttt cgactactga caacgaactc ttggaacaga ctgcaggaaa tatctttgct
    53281 tctgtcaaaa atccatatct ggaagcaagt gaatggggtt ggcaaattga tccattaggc
    53341 ttgagaataa cgatgaatga tatttatgat cgttatcaaa aaccactatt tattgtagaa
    53401 aacggcttag gtgcagtcga tacgcccgat gagaatggtt atgtggaaga tgactatcga
    53461 atcgactatc tggcagcaca tatccaggca atgaaagata cagtagaatt agatggagtc
    53521 gatctattag gctatacgac ttggggatgt attgatttag tatcagctgg tacaggtgag
    53581 atgaagaaaa ggtacggatt catttatgtc gatcgtgaca acgaaggaaa tggcacatta
    53641 gcccgttcga agaagaaatc attcaactgg tataaaaaag taattgattc aaatggagaa
    53701 aatctttcga atgattgatc aatgatttgt aaaagtctat tgtttttcag cggatttttc
    53761 gagtataata aagctattcg agaaacgcaa gcagaacaat agactttttt tcgcattggc
    53821 tgtaaagtat gcttacttga ttaagtaaag gagaatctac atatgtatga tgtaatcgtt
    53881 attggcgcag gtcctgctgg gatgactgct gctttatatg cttcacgttc aaacttatcc
    53941 gtattgatga tcgaacaagg cgcccctggc ggccaaatga acaatacggc agaaatcgaa
    54001 aactatcctg gtttcgattc aatcatgggt cctgatctag cttataagat gtatgaaggt
    54061 gtttcaagat ttgggacaga aaatgcttat ggaatcgtgc aagatatcaa ggatcacggc
    54121 agccacaaag aagttatttg tgaagataaa acttacgaag gaaaaacagt cattatcgca
    54181 acaggctgta tccaccgtaa attaggcgta cctggcgagg aagaatttgc tggacgtggc
    54241 gtttcttact gtgcagtttg tgatggtgcg tttttccgta acaaacgtct agtagtgatc
    54301 ggaggaggag actcagcagt agaagaagcc atctatttga cacaatttgc ttctgaagta
    54361 gtcatcgtcc accgtagaga tgaattacgt gcacaaaaga tcatccaaga ccgtgcattt
    54421 gcgaatgaaa aaatttcctt tatgtgggat acagtcgttg acgaaatcgt tgggaatgaa
    54481 atggtagtga caggtgtgaa agcacgtaat gtcaaaaccg gcaatgagtt tgatttggca
    54541 gcagacggag tattcatcta tgttggactg gatccattaa ctgcaccatt tagagaagca
    54601 gggttgacga acgaagctgg ctggattcca acagacaacg aaatgaagac gaatatacca
    54661 ggcgtttttg ccatcggaga tgttcgtgaa aaagatttgc gacaaatcac tactgctgtt
    54721 ggtgatggtg ggatcgctgg acaacaagtg tataaatact tggaatcttt agaagaaaca
    54781 actgcataag tttaaaaaga gtggaagaac ggtttcaatt gttcttttac tctttttttt
    54841 cgcgtgaaat ctactgaatc cgatttatgc gaatacttgc aaccgtgctt gataaaagaa
    54901 tcctttcttg atttaagcgt tttttagacc tcgtactttc atatctaata agtgaatgct
    54961 atacttatta agaaccatat ttcagaaaga ggtgttattt tcatgtcttg ggaacaagtg
    55021 tatgaacaat gggtacatga agaaaattta gaagaaaact taaaaaaaca attaattgat
    55081 ctgggtgaag atcctgaaaa acttgaagat gctttttatg caccgttgga attcggtact
    55141 gcaggcatgc ggggaatctt aggacctggg atcaatcgaa tgaacatcta tacggtaaga
    55201 caagcaacag aaggacttgc acgtttcatg gatacacaag gtgaagaaac aaaacgccga
    55261 ggtgtcgcta tcgcttatga ctcacgccat caatcaccag aatttgccat ggaagcagcc
    55321 aaaacattag cacatcacaa tatcccttct tatgtattcg aaagtttacg tccaacacca
    55381 gagctttctt ttgcagtccg tcatttgaag acgtttgcgg gaatcatggt aactgcatcc
    55441 cacaatccag cagcttacaa cggatataaa gtctacggag aagatggggg acaaatgcct
    55501 cctgcagacg cagatgcatt gacgaaatat gtccgtgaag tcagcaatcc gttaaaagtt
    55561 gaagtattat ctgacgacga agctgaacat agcggattga tcacgatcat cggagaagaa
    55621 gtggatgccg cttatctaga agaaatcaaa gcagtaacga ttgatcatga attggtggaa
    55681 acaatgggta aagatttgaa acttgtgtac acaccacttc acggaacagg aaaaatgttg
    55741 ggcgaacgtg cgttgaaaca agctggattc gaacaattcg tactagttcc agaacaagct
    55801 gtcgcagacc cggatttcac tacagtcaaa tcaccgaatc cagaagaaca ctctgctttc
    55861 gagtatgcga tgcgacttgg cgagaaagaa ggcgctgact tactgattgc aacagatcct
    55921 gatgcagacc gtttaggcgc agccgttcgt ttgccaaatg gctcctatga agtattgaca
    55981 gggaaccaaa tcggagccct gtttgctcaa tatattttgg aagcacacca atcgcgaggc
    56041 actttacctg aaaatgcagc ggtattgaaa tcaatcgttt ctagcgaatt gcctacagca
    56101 attgcggaac actatggggc taccatgttc aatgtattga caggattcaa attcatcgct
    56161 gaaaaaattc aacaatttga agaagatcat tcccatactt ttatgttcgg attcgaagaa
    56221 agttacggtt atttagttaa acctttcgtt cgcgacaaag acgcgattca agcattgctc
    56281 ttattagcag aagtagcagc ttactataaa aaacaaggca aaacgttgta tgacggcttg
    56341 caaacgattt ttgaaacata tggctactac gcagaaaaaa cgatttctgt gacaatgagc
    56401 ggacaagaag gttctgcaaa aatctctgct ttgatggaaa aattccgtga agaagcaccg
    56461 acagaatttg ccggagcaaa agtcatccaa acagaagact tcaaagagtt gacgagaaca
    56521 gaccgtgaag gaaacgtaga aaaactaacg acaccgccat ctgacgtatt gaagtatacg
    56581 ctggaagaaa atggctggat cgctatccgt ccttcaggaa ctgagccgaa aatcaaattt
    56641 tatatcggtg taaaaggaaa ctcttcagaa gacgcagctg aaaaagtagc cgcttatgaa
    56701 gcgattatca aaaaaatgac agaagaataa aaaaacttga gactgatgga aaaagtggta
    56761 aactttagaa gtcagtcttt tttcaagttg gaggtgaatg gattgccaga cacgcaaaaa
    56821 acaaaagtag agaaagtcag ccaactattc aaaatgttga gtgaccccac tcgtctcaaa
    56881 attctgctgt atctgaaaga cggggaacaa aatgtgacag caatcaccca agcagtagaa
    56941 atggaacaat ctgctgtatc gcatcaactg cgtttattaa gagaaaacca tgtcgtcaag
    57001 tcccatcgtg agggaaaagc gattttgtat agtcttgatg attctcacgt actggatatt
    57061 ttgaaccaga cgctcaaaca tgtggaacat caataacgat aggtgcaaat agagacaaac
    57121 tggactgtat caacgaatct tcttcgctga cacagtccag tttttttaag aaaaagacaa
    57181 gaaaaactgt tagtctgttt cttcggtggt ttcagttcca taataactat gatcttctag
    57241 gtctaaagca ttcaaaataa gtgaagctgt ttcttcgata gataaagaag cgacattgat
    57301 tactacgcag cccaattttt cataaagatc attcgcaaaa tcgagttcag ctcgtatttt
    57361 ttcgatatcg gaataagcag tatcaggatt taatccgtaa gagcgcatcc gttctttacg
    57421 gatattgttt aaaatatctg ggttattcgt cagtcccacg atttttttag gatcgatctc
    57481 ccaaagctgt ttgggaatat gtgcttgtgg gatcaaaggt agattggcaa cctttaaatt
    57541 tttgttagct aaaaacaagc tcaaaggcgt tttggaagtt ctggatacgc ctagcaaaac
    57601 gacatctgct tctagaaacc cacgaggatc tttcccgtca tcgtatttta cagcaaattc
    57661 cattgctttg atccgtttga aatagttctc attcaataaa tgaagggcgc caggttgccg
    57721 aatgggcttg ataccagtta agcgctcgat ttctgatatc ggcggagtca tcacatcaaa
    57781 actaaataat tgatgttctt cacaaaaaga acgcgtcatc tcgatcaatt cttgattgat
    57841 caatgtgtgc aggacaaggg ctttttcttt tagtgcatct tgcagtgcct gcatcaatgt
    57901 tttctcatct gtcacaaatg ttctgcggaa taaattgaac tcaacggtag gatattgggc
    57961 catagtggct tgcgctaatt ttgtcgcagt ttctccagca gagtcagaga tgacaaaaaa
    58021 agtaacgatt tctgtagaat cggaagacat ggataaaacc tcacttgcat tttttttatg
    58081 tttattatac catactctag aatgtgtact tcgtaatcat tttgatttgg aggaataagt
    58141 catgaaaaaa cagacattga ttgaacaatc actagaaaca ttgaaaaata acggttttaa
    58201 atatactaaa aaaagagaag cattgttaac ttatttagtg aagcgcaatc gctacgtatc
    58261 cgctaaagaa gtttttgact atatgaatca ggaattcaaa ggagtaagtt acgatacgat
    58321 ttatcgaaat ctccatgatt ttgcagaaat cgatttactg gaagaaaccg atttaaatgg
    58381 agagatgaag tttcgttttc attgctgtca aggggaaatc ggccatcacc atcactttat
    58441 ctgtacggtg tgcggaacaa cgaaagaaat ccatatgtgt ccaatgaatt atttcgaaga
    58501 acagctcggt ggatgtacga tcgaagggca tagattcgag attttcggaa gatgtgaaaa
    58561 ttgtcaataa ggatttaaat aaaataacat ttgataattt taaaaaaaag caagatttcc
    58621 ttgacggtca aaacagcatt agatataata tttacaggac agtcttgttt tttgagatat
    58681 atttcaataa caattacggt tactttttaa gctcggaggg agggaattaa catgtcaaaa
    58741 acagtcgttc gcaaaaacga atcacttgac gatgctcttc gtcgcttcaa acgttccgtg
    58801 tcaaaagcgg gtactttaca agaatctcgt aaacgtgaat tctacgaaaa accaagtgta
    58861 agacgcaaga aaaaatctga agcagctaga aaacgcaaaa aattctagtt ctgtgatgaa
    58921 ttggagttgg tagtgtgtca ctactaagta gattaaacga tgacatgaaa acagcgatga
    58981 aagcgaaaga taaggaatct ttacaagtca tccgaatgat caagtcatct atacaaaacg
    59041 agcagattaa agaaggtcac gacttgacag aagaagaaga attaacggtg ttgtctcgcg
    59101 aaatgaaaca gcgacgtgat tctttgcatg agtttgaaga agcaggacgt gacgatttag
    59161 ctgaaaaagt aaaaagtgaa attgtgatag tcgaaaaata catgccggaa caactttccg
    59221 acgaagaaat tcgacaactt gttcaagaag caatcacaca aactggtgct tcatcaatga
    59281 aagaatttgg caaagtcatg ggtgcgatca tgcctaaagt caaaggaaaa gcagatggaa
    59341 accaagtaaa tgccattgtg aaagaactat tacaatctta attttatcat ttaaactgcg
    59401 gacaatcatt gtccgcagtt tttttgtgcg cgtaaaaaag agaacaaaaa aatagctaga
    59461 cgaaagaata ggattctttc atctagctgg ttttttcctg tttaatattt cttatgttaa
    59521 attgtgtttg atttggttat tcttctggta gttcatttgc ttcttgttta taccctgata
    59581 ggcgataggc gtaataaaac agtaggctgc cagcgtaacc aagtaacgtt gggaccatcc
    59641 agccaagtcc taatgcagca aaaggaacgt gatgatgata aaattcgagc atgcttgcta
    59701 caactctttg ttgactcact aaagcaggcg aatttgccag catatccaat acggcaggaa
    59761 cagcagcaaa caacatcgtt acttgataga caaatggtgt cttttggaaa aattttgctg
    59821 ttaaggaaag caagatcaac gcaagcgaca atggatacaa aagcatcaat actggtaaag
    59881 accattggat gatattgtct aaaccagcat ttgcaacgac aaaagagacg aaagtcgtta
    59941 accgcagcca tgtcatataa ctgactttag ggaacagttt atggaaatct tgcgcaaaag
    60001 aaacaacgag tcccatagcg gtagtgaaga cacctaatgt gaccatcact cctaagaagc
    60061 tagcgccgaa gttacctaaa taaaaattag tgatctgaga aagtgcagtt ccgccatttt
    60121 ctgataaaga catcttattc aaactcatcg caccgagaag aacaagacca aaatagatca
    60181 tgacttctag cgcaacgctc aggctaccag cttttgctgt taattctgtg acttttttat
    60241 ctttgaaacc aaggtcacgg actgcatgga caaaagtgac acttaaggca agcaaggcta
    60301 ctgcatcaac cgtgttataa ccttctagga caccatttgt taaagcgttt gtttggtaag
    60361 tatctgcagc agattgattt agatttccca ttgggtggaa taaagaaacg acaaaaatga
    60421 ttgctaacaa tgcaagaaac aaggagttta gatactttcc gacgtatttg accaaccgtg
    60481 tgggatggac agtaaggaaa taagcaagta aaaagaaaag agcagagaat aaaaacatac
    60541 ctaatgaatt atatttttca ggcaaaaatg gtttagcagc catttcaaaa gcagtagcag
    60601 ctgtccgtgg tgtaccgaaa aatggtccga tcgtcaaatg aacaaggatt aagaacaagg
    60661 cagcatacca ttttccgaca ggtttagcta agtcatacag accatcactt tttgtcacta
    60721 ctacagctaa gatagctagt aatgggaaaa gagcgcccga gatggcaaat cctacgccag
    60781 cactgaccca gtttgcgcca gccatttgac ctaagtggac aggaaagatt agattacctg
    60841 acccgaagaa catcccgaag ataagtgaac tgaccatcaa tagttgtttc catgaataag
    60901 tttttgtttc ttccataaat ttttcctcca ataattatca ttttgattga cgatataaac
    60961 gttgcagcaa ttagtaatgc cactaatatc agattccatg aaaaccacca ccttaaaata
    61021 taaaaaaagc ccttttccac ttgggaaaag ggctctagat tctagaacgg taccaccttt
    61081 tttcgcttaa aaaagcttct tcattcacgg acgctttttg ataaagttat ccgctagcct
    61141 gataatggag gcagccacaa tgatctacgt tttcgacatt gactcggaaa tgatgttcgt
    61201 ctattcgatt tattgtcatt tctcacttac atgactcact gggaataaat cgttgaatag
    61261 cctactgttt tcctcgacgc tttgctgtat gtttatttgt tgacgtaata atacacctgt
    61321 tttttttgaa atgcaagaat attttaacta ttttgttttc tttttttgaa acaagaaaaa
    61381 tacctagaga atacctgctg aatgaaacgg aatattttgt tctccacttt catataactt
    61441 acattctcag cttcttttta tcaaaacgag acctttagcg aatggaaacg gctatttttt
    61501 aacgtttctt ggtacgtatg gttaaacgac tcttttacaa ccttttctta tttgctatat
    61561 agtttcttct gagtatggta aaataggaaa gaatgaatcg aaaaaaaggg gatgtctatt
    61621 tttgacggaa gagtctagtt ctttagagat tagattgaca gctgctgatg atgtcagcat
    61681 gcttttaggt gcacacgaca agcatataaa actgatcgag gaaacaacgg aaacaaccat
    61741 ccatacacgt ggagaaatca ttcagatcat tggtgagcaa tcccagattt ccttggctgc
    61801 ctcagtcatt cggacattac aggaattgat caaacgagga atccacgtaa gtacgccaga
    61861 tgttgtgacg gcattgaaga tggggcaaaa aggtacatta gattattttg ttgagatgta
    61921 tgaggaagag atcgtcaaag accgtaatgg aaaacctata cgggtaaaaa acagcggtca
    61981 aaagaaatac gttgaatcgg tagccaaaca tgacatcgta tttggtgtag ggccagccgg
    62041 aacagggaaa acgtttcttg ccgtagtatt agcaattgct gccctaaaaa aaggcgaagt
    62101 gcaaaaaatc attttgacgc ggcccgctgt ggaggcaggg gaaaacctag gctttctacc
    62161 cggggatctg aaagaaaaag tggatcctta tctgcggcct gtttatgatg ctttatacca
    62221 aatttttgga ttggatcata cgaaccgatt gatggaacga ggcgtgatcg aaatcgcacc
    62281 tttagcttac atgcgggggc ggacattgga tgatgcattc gttatcttgg acgaagcaca
    62341 aaacacgaca gtcgctcaga tgaaaatgtt cttgacccgt ctcggttatc aatcaaaaat
    62401 gattgtcaat ggggatacaa gtcagataga cttgcctcgt gggacgacaa gtggtcttgt
    62461 tcatgcagaa cgaacattaa agcaaatcaa aaaaatcgat tttgttaatt ttgaagcaag
    62521 tgacgttgtt cggcaccctg tagttgccga aatcatcaaa gcttatgaaa aggcggatct
    62581 tcatcaagaa taagctttgc ttaggaggat aagcacatgt tgaataaaac actgcagaaa
    62641 ctacaaaaaa aactcggaaa ggcagcattg cctttccttc tgttcttgtt ttttatcatt
    62701 gtctgcggcg ttacatttag tagtgtcaaa caaaaggcca atgatttcag agaaggccaa
    62761 gtagcagaag agagtatccg tgcgaataaa acgattgaga acacggaaga aaccgaacaa
    62821 agaagaaaac tggcagcaga agcagtgact ccagaatata cttatcaaaa agatctggca
    62881 gatgaccaga acaatcggat caagcaatta ttcgaattga tcgataagac gaatgatacc
    62941 atcaacaaat cttataatga caaggtagat aaggcgaagg aagatgaaaa tatcccgaaa
    63001 cctactacag aagagcggat tgctacactg aagaaaaatt ttgaaaacgt gaatgcagaa
    63061 aacgttgcgt tttaccaaaa actgccgaac aatttttatt cgacgatatt tgaaatgact
    63121 gaaacggaaa ttcacacggt tagggatgaa agtttgaagc tcattgacga acagatgagt
    63181 aaacaagttc gtgaaagcga attggaagct tttaaacaag aagcagaaga tcagattcaa
    63241 tatttgaatg tcacaacaga gcagcaacaa atgattcgct acttagtgga tcaaggcatt
    63301 gtagttaatg atgtattgaa tgagaaaaag acagaagaat taaaacaatc tgcacgagaa
    63361 gctgtacaac cagtgatgat cttccaagga gaaattattg ttcgcgaagg aaatcaaatt
    63421 gatgcttcag cgatgaaaaa attggaatta ctaggattga cgaatcaaac gacttctatt
    63481 ttcccactca ttgccatggt tttagctgtt ttgcttcaaa tcggtgtact gatttataat
    63541 tcacttcaat tccaaaatgc aggagaaaga acaaaatacg tacttttcta cgtgacggca
    63601 atgtcagtaa gcgtcgtact aatgaaattt ttccaactct tccaaacaga acaagctgct
    63661 tatattccgc tgttttaccc cgcggctttt gctccacttg ttttaagctt tttcttgaat
    63721 cgaagagcag ggatcatggc tggtattttt caagcagtat ccgcactttt cattttttat
    63781 gattcgattg gcacgaactt tctgacagtt attttgatgt cctatgtatt ttctggtctc
    63841 atggcaacga tcgtcagacg caaacggatc tcagaacaag ggttatccgc tgctatgtgg
    63901 gtcattattt ttcctgtgat gatggatgtc attttgatta tctaccaagg aatgagcttt
    63961 ggggatgcaa cgacttggtt aacgctagta tgcggattca caggtgcact gttctcattc
    64021 ttgctgacga taggtctgca cccctatatt gaattgatgg tcaatgatga tagtgtgatc
    64081 gtattaaatg aactcagcaa tcctaatcat cctttattga aacagttgct ggaagaagca
    64141 ccaggcacgt atcaccatag catgatggta gccaatctaa gtgctaatgc tgtagcagaa
    64201 atcggtggac gttctttact aactcgagta gcttgctatt atcatgatat cggcaagatc
    64261 aaacacgcca gctttttcgt tgagaacttg ccttcaggag cggaaaatcc gcacaatttc
    64321 ttattgccag aagacagtaa acatattatc tttggacatg tgacagatgg tgccaaaata
    64381 ttggaagatt ataatatgcc gcaaatggtg atcgatattt gcagacagca tcatggaacc
    64441 acgctgatgc gttatttcta cgttaaggca aaggaacgca acccggaagt cacggaagag
    64501 caattccgtt atcctggacc aaaacctcaa agccgtgaag caggaatcgt caatatagct
    64561 gacagttgtg aagcggcagt acgagcaatg gaccatccaa cgagcgaaaa aatcgaaagt
    64621 tttgtccata atcttattga agagcgtatt tccgatggac aattggacga cagtggattg
    64681 acgttaaaag aaatccgtaa agtagaaaaa tcattgatca gcggtttaag cagtaccttc
    64741 cattctcgga tcaaatatcc taaaatgaaa tcggaagttg agaagataaa agaagaacaa
    64801 gaagaataga aagaagcgat gtataatgga tattactttc atggatgaaa caaaccaagt
    64861 ctcagaagag aaaatgaaag aaatcgacga cttgctgcaa tttgcagcat catatttaga
    64921 gttgccagaa gaaacagaaa tgtctgttac tttcatggac aatgcagcga tccaagtaat
    64981 caatcgtgat taccgtggaa aagatgcacc gacagacgtc atcagttttg ccttggaaga
    65041 agagggcgaa gacgaaatcc cagtgatttt tgaagatgga gagaatccat tgcctcgtgt
    65101 actgggagac atcatgatct ctactgaacg tgctgcagag caagcagaag cttacggcca
    65161 ttcgtatgat cgggaacttg gctttttagc ggttcatgga ttcttgcata tcaacgggta
    65221 tgaccatatg acaccagagg atgagaaaga aatgtttggt ttacagaaag agatcttaga
    65281 tgcctatgga cttaaaagat aaaaacgtag agaaaaacaa acatttcctg atgtccgtgg
    65341 aatttgccgt ccaaggagtc aaaaccgttt ttgaggaaga acgcaacatg aggaaacatg
    65401 ttgcgttcgg aatcctcgct ttgtttatgg gcgtgatttt ccagcttgat cggatagaat
    65461 ggttatggct gcttttagct gtttttctcg tatggatagt agagatcatc aatactgttt
    65521 ttgaaaatgt cgtggacatg tttacggatt ttcattttca tccgattggg aaaaaaatca
    65581 aagatatggc agctggagca gttttagtga ccgcttgttt ttccgtcatc gttgggttga
    65641 ttttatttgt ccctaaaata tatcatttgt tgtttaatta aaaaggagaa cgtatatgcc
    65701 agaacataaa tcagggtttg tcgcaatcgt cggccgcccg aatgtaggaa aatccacatt
    65761 attgaatcga atcgtgggtc aaaaaattgc catcatgagt gataaggcgc agacaactag
    65821 aaataaaatc caaggagtat atactacacc agaagctcag ttgattttta ttgacacacc
    65881 aggaatccat aaaccaaagc atcgtttagg agattttatg gtagaagcag cttacagtgc
    65941 gttgaaagaa gtggacgcgg tcttatttat gatcagcgct gatcaaaaac gcggtcgggg
    66001 agacgatttt attattgaac gtctaaaaaa cgtgcattct cctgtttatc tgattatcaa
    66061 taaaatcgat aaaatccatc cagacgagtt gttgggaatc atcgaagatt attcaacgca
    66121 gatgccgttt gcacaagtga ttccaatttc tgctacagaa ggaaacaacg tcgaacgttt
    66181 gatggatgta ttagtcagtg aaatgccaga gggcccacaa tattttccag atgatcaagt
    66241 gactgatcat cctgaatact tcatcgtttc tgaattgatc cgtgagaaag tcttgttttt
    66301 aacacgagac gaagtgcctc attctgtcgc agtcgttgtt gattcgatga agcgaaacga
    66361 gaatgataaa atccatatcc aggctacgat tattgtcgaa agagatagcc aaaaagggat
    66421 cattatcggc aaaggcggaa aaatgctgaa gcaagtagga acgaaagcga gaaaagatat
    66481 cgaaaatctt ttaggagaca aagtctttct agaactttgg gtcaaagttc aaaaagactg
    66541 gcgagacaaa aaagtctatc tgcaagactt cggctaccga aaagacgaat attgatattc
    66601 tgagcttctt gtaagttagg tatagaaata aactggataa aaatccgcgt gttgagaaaa
    66661 gctgcggaag atcgcaacat aaatgatctg gatcggcaga atctgctttt ccagatttat
    66721 tttttaggag ggagaatatg agccagcttg cagaatcaaa aggcttgatt ttatctagcc
    66781 gtaattacaa agaaaaagat aaattagtca aaatctttac ggaatcttct ggcaaaatga
    66841 tgttttatat caaagggatc catcggcaaa atcagccctt agctccggct ttgctccctt
    66901 ttactgaagc agtttatatc gggaattttc gaagtgaagg tttgtcattt ttaagcagtg
    66961 tcaaagatgt ccacgcgttt cgacacatcc aagaagacat ctttttgagc ggctatggca
    67021 catatttatt gaatctagta gatgcggcaa tcgaagatca tcagtacgac ccgcatttgt
    67081 tccagtttac gcaacaagca ctgcaactga tggaccaagg agatgatgca gaaatcatca
    67141 cgaatatttt tgaagtccaa atactgcaac gcttcggaat tgcaccaatc tggacacact
    67201 gcgtcgtatg tggggaaaca aaaggaaaat ttgactattc ttccaaatat ggtggtgtga
    67261 tatgcgaaag acactggtcg atggatgatc accgttatca tgcagatcct cgagctgttt
    67321 atttcgttcg gatgttttca gctatttctt atgataaaat cagtggaatc catctgaaag
    67381 aagaaaccaa acaagcaatc cgtgaactca tcgaccagct gtatgatgaa tatgtcggta
    67441 tccatttgaa aagcaaaaag ttcattgatc aaatgaagtc ttgggcacac gtattaaaga
    67501 cgtcagagaa agacatgtct gaaaaagatc tacctaaaaa agaagaatag taaatgaaac
    67561 atacaacaaa aaatcaaaac catttgacaa acacagggag ttccgtctat tattaaccgt
    67621 agatcaaata gatcgttcac tgagaggatc tattatagaa acaagaaaac tgcaaaatga
    67681 atacgtacat tgaccaagat cagtaaaaaa cacgtggttt agcgagttcg ggagagtggg
    67741 agccggaccc aaacgatttt tgaagaggca cttgcgagta cgataaatga agctgccgaa
    67801 tctttattcg gaagtagggt ggaaccgcga attattcgtc cctatgtctg taatcagaca
    67861 tagggatttt ttgtcgtttt ctttcctgta ttcttatttg acagcaacgc tttaaaattg
    67921 aaaggggcaa aacagatgag taaaaaattg accgtccaag atatgatttt aacattacaa
    67981 aaattttggt catcaaacgg ctgcatgctg atgcaagcgt atgacacaga aaaaggagca
    68041 ggtacgatga gcccatatac ctttttacgg gcaatcggac cagaaccatg gaatgctgct
    68101 tacgtagagc catctcgccg accagctgat ggacgttatg gcgaaaaccc aaaccgtttg
    68161 tatcagcatc atcaattcca agtcgtgatg aaaccttcac ctgagaatat ccaagagctt
    68221 tatttagaaa gcttgcgcct tttaggaatc gatccactag aacacgacat ccgttttgtt
    68281 gaagacaact gggagaaccc atccatgggc tgtgccggtg tgggttggga agtatggctt
    68341 gacggaatgg aaatcacaca attcacgtac ttccaacaag ttggtggact gccttgtaaa
    68401 ccagtaactt ctgagatcac ttatggatta gaacgtcttg cttcttatat ccaagaagtt
    68461 gaaagtgtct atgatttaga atgggctgat ggcgtgaaat atggcgagat cttcaaacaa
    68521 ccagaatatg aacattctaa atattctttt gaagtaagcg accaagactt gctactaagc
    68581 aactttgatc gttttgaaaa agaagctaaa cgctgtatcg atgaaaatct tgttcatccg
    68641 gcgtatgatt atatcttgaa atgtagccat acattcaact tattagacgc ccgcggtgca
    68701 gtatccgtaa cagagcgtgc cggttatctt tctcgcatcc gcaacatggc tcgtgctgta
    68761 gccaaaatct ttgtggcaga acgcgaaaaa ttaggattcc ctttgttgaa taaaaacaca
    68821 gcaaatccag aaaacgtgaa ggaggctgaa taagatggca aaagacttat tacttgaaat
    68881 cgggctggaa gaaatgcctg ctcatgttgt cacaccaagc atgaaacaat tagaagaaaa
    68941 aacagcaaaa tttttagatg aaaaccaatt gacttatgat acgatcgaaa cattttccac
    69001 accgcgccgt ttagcaatca aagtaacaaa cattccagaa cgacaagcgg acagtgaaga
    69061 agaagtcaaa ggaccagcca aaaaaattgc tttggacgca gatggaaact ggacgaaagc
    69121 agcggaagga tttgtccgcg gacaaggtct gacgacagac gctatcactt ttagagaatt
    69181 aaaaggtgtg gaatatgtct atgtaacgaa atacactcat ggtaaggaat ccaaagaagt
    69241 attgacaggt ttagcagatg tcattaaaag cctgactttc cctgtaacga tgcattgggc
    69301 agaatacgat tttgaatata tccgccctat tcactggatc gtggcattgt tagatgacga
    69361 agtaatccca ttcgaaatct tggatgtcaa aacaggcaga gcctctcgag gtcatcgttt
    69421 cttaggcgaa gatgtgacat ttgcgcatgc aaaagaatat gaagaaaaat tgaatgaaca
    69481 attcgtcatt gcgtctccgc aaaaaagaca agaaatgatc gttacacaag ccaatgaatt
    69541 agccgaaaaa aatcattggg ttattgatct agacgaagat ttactagaag aagtcaacaa
    69601 tttagtcgaa tatccaactg tcttttctgg cgacttcgaa gaaaaatatc tttctgttcc
    69661 tgaagaagta ctagtcactt ctatgaaaga acatcaacgt tatttcgagg tacggaatga
    69721 tcaaggtatg ctgcttcctc attttatttc tgctcgtaat ggtgatcggg taaaattgga
    69781 aaatgtggtt cgaggcaatc aaaaagtatt gacggctcgt ttagaagatg cggaattctt
    69841 ttataatgaa gacaagaaag cttcaatcga ccaatacgtt gaaaaactaa aaactgttac
    69901 tttccatgaa aaaatcggta gtatctatga gaaaatgcaa cgggtacaag tcattgcccg
    69961 tatcattggc gaacaagtag gactatctga ggaagaattg atggatctgc aacgagctgc
    70021 tgaaatctat aaatttgatc tagtaaccaa catggtaggg gaatttcctg aactgcaagg
    70081 gatcatgggc gaaaaatatg ctctgctgaa aggcgagaaa cctgctgtag cgacagcgat
    70141 tcgtgagcat tacatgccta tatccagtga aggagagcta cctcagtcta cagtaggttc
    70201 tgttttagcg attgcagata aattagatag catcttttct ttctttgctg ttgggatgat
    70261 cccaagcggt tcaaacgatc cttatgcctt acgtcgccaa gcatacggtg tcgttcggat
    70321 catcgaatcg aaaaaatggg cctttccttt gtctgttctg caagaaacga ttagcgatgt
    70381 tatcagtaaa gatacggatc gtttcggcat tggtttatca gaagggcaac aacaagtgat
    70441 cgactttatc aaaggccgtc tgcgccaatt attgacaacg aagaatattc gtcatgatgt
    70501 catcgatgct gttttgaatg cggaacaaaa agatctgaca aaagtatttg cagcagcaca
    70561 attgttcaaa caacatcttg cagatgaaga cttcaaacct tcaatggaag ctttgacacg
    70621 tgtcgtgaac ttagcgaaaa aagcgcagct tgaaaaacaa tcagaagtag atcctgagct
    70681 gttcgaaaac gacgcagaga aagaattaca caaagcagta gaagcaatga aagcaagttt
    70741 tgctgaccaa acgatccacg aaaactacga ccagcttgtc gctcttcgtc cactgatcga
    70801 aaattatttt gatcaaacga tggtcatggc tgatgaacca gcagtacgta agaaccgact
    70861 aacacaactt agtcaaatag ctgccatggc tttgtcatta gcaagcttgg atcaaatcat
    70921 cacaaaatag tcttttgttt gaaaaacaga gagcatgcac gaaacgtgct gtgtctctgt
    70981 ttttttgtta taaatataaa tagctgttat taattctatt ttaataattt ttgttatttt
    71041 attctttgtt cgaattttca aaatgctcat gaaaagagag gaaataaaaa tgaaaacgat
    71101 taatcatgat cttgttactg ttttatttac ttttaaatta ggaatttccc tagtcaagaa
    71161 aactttttgt ggtatgatta tatagggaaa acctgtttat aaatatttct atgaatatat
    71221 tagtttaatt atgcttttaa aataaaaagc gctcattctg atgggaatga atcacatctt
    71281 tatttatcta tcatcaatat ttaggagtga atctatggaa aagccgttcg tcaagttatt
    71341 ggctactgtg gcggtaggca caggcgctat tgtgatttgc ttgtttggat accatttcaa
    71401 taatcagcgg caacatcatc aacggatcaa ttacgcagaa tctgccatca ccaatcaaaa
    71461 agacaccgtg acctctttat caaaagaagt agataagctg tacagtacaa aagaaaaaat
    71521 tttcctaaat cctgaaataa cagaagaaac agtttcaaat ttgtcacata aactatcttc
    71581 tatcaaactt tcggcagatg attatgacat caaggaatca gaattaccta aagaagctgc
    71641 tgcgatccaa gaagaaaaga aagcagtctt aacgcagtta gaggatgcag aatcaaaact
    71701 gaagatccag acagctgtca acgatttatt tactaaaaat gtatcaaact ggcagcaagc
    71761 tgtagacgat gtcgtcatca aagaaaaggt gacaagtgca gatgtcgctc gtgtacgtga
    71821 aaatatgagc ttttttaaag atagtccttg gaaaacggtt gttatgcaat atctaggatt
    71881 cgctgatact cagatcgcgc aagtcacgca actagatcag ctattcgata cgatgctgaa
    71941 agacggtcaa gtaacagcta ctgctactta cgatcaatat ttgaccgcac tcagtctcat
    72001 cgaacagatc cggaatgaaa aaataagagc cgcatacgcg tcaaaagcag aaacagtcgc
    72061 tcagcaaatg ggctatagca atacgagcta ttaataagcg agaattcaag caaggctagc
    72121 aaacggagga aactatggca aaacgaagaa gaagatcaag agggaaaaag atatttctaa
    72181 ttattttagc aattcttgtc gtattagtat tagcaatcgt aggagtagga gcaaaattat
    72241 atctagatct ctcacattct attgaaaaaa cttacgaatc agtggaacga acccaaggag
    72301 aacgaacaaa accagttgat ctggacaaac aagaatcttt ctctgttcta ttgatgggga
    72361 ttgatacggg agatttaggt cgggtcgatc aaggaagatc agatacgatg atggttgcca
    72421 cagtcagtcc tgaagacaaa cagacaacga ttgtcagcat tcctcgtgat acgtatgtcg
    72481 acatcgttgg acatgacaca aaagacaaga tcaaccacgc ttacgcattc ggcggagctg
    72541 ccatggcgat ggataccgta caaaaatatg tagacatccc aatcgatcac tatgtttcaa
    72601 tcaatatgaa aggtctaaaa gaactcgttg atgcagttgg tggtatcgaa gtcaataatg
    72661 acctcacctt ctcacaagat ggctatgatt ttacgatcgg taaaatcaca ttgaacgggg
    72721 atcaggcatt gtcttattct cgtatgcgtt atgaagatcc aaatggtgac tacggcagac
    72781 aagaacgtca aagaaagatc gtagaaggtg tagcgaaaaa agtgttgagt ctgaacggtg
    72841 taagcaacta tcaatccgtt ttgaatgctc tagagtcgaa tatgaaaaca gacatgagct
    72901 ttgatgaaat gaaaaagatt gctttagatt atcgggatgc atttggtacg atcaagcaag
    72961 atcagctgca aggagaaggc ttcatgcaag acaatatctc ataccaaaaa gtcagcgatc
    73021 aagaacttga tcgtgtgaaa aaagaattaa aagatcaaat gaatctagag aataaatagg
    73081 aaagtggaga aaaaatggaa gagacaatca gcttgaaaga aatttttgaa acattacgca
    73141 aacacgtgat aacaatcctc atctcaatgt ttgctggctt ggccatctca ggcatcgtga
    73201 cgtttttcat cttgacgcca aaatacagct cacaagccca attgatcgtg acactgccgc
    73261 aatcagatac tgcaaatgtc aatgatgtca atacgaacct gcaaatgatc aatacgtata
    73321 aggatatgat cgtcagtgat ctagtactaa atgaagtcaa agatcgttta gaaacagaag
    73381 acaatgtaaa aatgactgct ggacaaatca aagatgccat ctccgtcaat cagtcacaaa
    73441 attcacaaat gttctcgatc caagcaacaa gcacaaatgc agtgacggct caacaaatcg
    73501 ctaatacaac gtcacaagta ttccaagaga acgccaaaga cgtgatgaat gtcgataaaa
    73561 tctcgatcat ctcaggtgca gttgccaata cgacacctgt ttcgccaaat aataaattga
    73621 atctagtgat tggcttagtg ttaggaatga tggtaggcgt aggtctagct ttcttatttg
    73681 aattactcga ccggacagta aaagatgaaa aatatgtgac ggatacattg ggcttcccga
    73741 tcttagggac tgtaccagaa atgtcagcga aagaattgaa tgctggtatc cgtaaaaaag
    73801 gaccagctcc gacacaacca aaagctacaa acgataaaac agcgccatca cgcagaagcc
    73861 gctcaagagt ctaggaggaa agaaaaatgg cacgaacaca gaaacaaaaa gcagcccaaa
    73921 ctcatgcagt cagtttggtc acactcacaa acccctcttc accaatcgct gaacaatatc
    73981 gtacgattcg gacgaatatc cagtttgcct ccgcagctgg tcagcaaatt aaaacgattg
    74041 ttgtgacttc atcaggacct ggagaaggga aatcgaccac tgcagcaaac attgccgttg
    74101 tttttgccaa atctggtcaa cgggtgctgc tagtcgatgc agacttacgt aagcccgtca
    74161 tctataaaac gttcaagttg aacaacgcaa gcggattaag taccgttctt agttcatcag
    74221 gaagtgtagc agatgagatc caacgaacac cagtcgagaa tttgtcgatt ctgccaagtg
    74281 gacctaaacc accaaatcca tcagaactac taagttcgcc aagaatggat cagattttag
    74341 cagaagcaag acaactattt gatgtagtga tcttcgacat gccgccagta gttgccgtaa
    74401 cagatgcaca gatcatgtct tccaaaacgg atggcacctt attggtcgta cgtgaaaata
    74461 tgtcacggaa agaatcattg actaaagcaa aagaattgct ggatatggta caagcgcgag
    74521 tccttggtgt tgtatacaat ggggcagaac acagcaaaga tgctggctat tactacggga
    74581 attaagaaat aaaaagagag gagacaagag aaatgatcga tctacattgc catatcctgc
    74641 caggaatcga tgacggagca gaaaacatag atgccagtat cgccatggct gaaaaagcga
    74701 ttcaacaagg gatcacacat attttatgca caccccatca taataatgga aaatacagca
    74761 atgaaaaatc acaagtcatt tctctagtcg cttcgttaca agcagaatta gagaaaagac
    74821 aattgccttt gacgttattg gaaggacaag aagtacgtat taccggaaca ctgatcgaag
    74881 acatcgaccg agatgaaatt ttatttacag atttagatga tacatatctt ctaatcgaat
    74941 ttccgacact ggaagtgccg ttatatgcag aacagctctt ttttgagttg ttacaactag
    75001 gaaagacacc agtgatcgtc catccagaac gcaacgcgca ttttcgtaaa gacccgaatc
    75061 atatgctgcc attcctagat atgggatgct tggcccaact caccgcacca agttatgtcg
    75121 gtgtatttgg aaaagacatc cagaaaacag ccaaaatgat ggtcaaacat aatcttgtgc
    75181 agatggctgc ttctgatgcc catggggtct ctaagcgtac cttttactta aaagagtgct
    75241 atgagcagat tgctaaggac tttggtaagg aaaaagtagt acagatgaaa caagtcgcaa
    75301 aggatctgat caacggggat gaaatcagtt atccagtcta tgaagaggta aaaaagaaga
    75361 agtttggttt attttagttc tttattcgta gataaaaagg aaatgaggag agagggaaag
    75421 tatggaaagt gaagatttat tatctcgtat tagtgaagta catcctaatc aagtttggtt
    75481 ggataaagaa aaagttgaaa aacgatatat ctatttattt tttaaaagaa tgattgatat
    75541 tgtaggaagc attattggtt tgattttagc ttctcctttg atgttctatg tagcttatcg
    75601 aatcagaaaa gaagagccag gaagtccgat atttttttgc catacacgtg ttggtaaaaa
    75661 tggaaaaaca ttcaaaatgt ataaattccg ttcgatgtgt ttagatgctg aagaaaaatt
    75721 ggaagaatta cttgatcaaa atgaaataga aggtgcaatg ttcaagatga aagaagatcc
    75781 ccgtgtcact aaaataggta aaattatccg tgccaaaagc attgatgaat tgccacagct
    75841 atggaatgtt ttaaaaggcg atatgagtct aattggtcca cggccacctt tggagaggga
    75901 agtagctgag tacacgagat atgatcttca acgcttgtat gtaaaacctg gttgttcagg
    75961 tttgtggcaa gttagtgata gaaataatgt tagtttccat aaaatggttg agttggattt
    76021 agagtatatt agtcatcgaa gtttaatgta cgacttgagg ttgattttga aaacgtttta
    76081 tgttattgtc aagcccaatg gagcatatta gttagggata gaatgatgga ttgttaataa
    76141 aaaagattac acatgttcat attatcgttt caaaagtaat aactgtaatt atggagggtt
    76201 tgaatcactt gtagaaaatc taaaaaaaga aaggtgaatg gggcaattca gtatcattat
    76261 tcttatataa aagaaaatat tcgaaatttt gggatgatac cactcttaaa tataatgatc
    76321 taaatgcttt aatattggta tatccgatat aggaactgac gagctattta gtatgataca
    76381 aaagtatatt gatagccatt aataaatttt ccaaaaaata atgacagcac accaattttt
    76441 ataatttagc ttgttgaatc ggtatgttca tgggacggtt caaaaaaaga atcgaaaaat
    76501 aaacggtaaa cttttagtga atcctaacgg atatgagtgg ttacgagcaa aagggagtta
    76561 tccggtccca aaatattgta attattctga aacattaatg gaatggtatc aactgataat
    76621 aacataataa gagataaaat tgaggaatat ctatcatttt ataacaaaga gaatgctgca
    76681 gagtgtttac ttaaaatgtg ggagtaaggt atttgtgact ttagaagagg ctatttttta
    76741 tctataagaa tttattatcc actgtgcaga aatgaagatt atagttcatc taaactttta
    76801 aactttgtaa tatattggat gttcataacg gaaaataata tttggaaaag tacgttgtta
    76861 taaagtatgt accactatct taaatattat gaaactaaga caattttttt tgaaactatc
    76921 tatagatata tttactggaa tttttggagg tatagaaaca gttatcattt tatctttgta
    76981 ccaccaatga tttatatcag ctcaacagag tgaattgtat ttacgcaaga atgcgtagga
    77041 tgaaatggat agataattaa gatatatatg tatatggtga ctgaagacaa aaaaacagaa
    77101 ctaatgaaac tgaataaaaa tatagagtga actagtttct aagagggatt ttaaactaga
    77161 tttcattggt aataaaattt ggacagatgg aaaaacatca cataaggaag cagattttgt
    77221 tgtcattttt gttcctacaa actatggtag tgaatgtgtt attgaattag tgatgtctgt
    77281 tacttcagag gcattgggga taattaaatc cactatacct gtaggatata caaaaagtgg
    77341 aagaaaaaaa tattatcaat agattttatt tagttcagaa tttttacgtg aaagtagatt
    77401 attaatgata atctttatcc aattagaatt attatcgtag gatgtgatga tgaaagccgt
    77461 gaaattgttc aagtatttac caatgtctcc ctgccagtaa atccgcttag aatttgagcc
    77521 ttgaaaatgt gcaatgataa aaaacaaaaa ctcgctcaaa atagacagtt tggggtggga
    77581 atagaccaat aatgtaccaa acaaacttcc atttggggtg aaaataggtc aataaagcga
    77641 tgttaaacat acctttggag taagaataga gggtaataag tgaatttata aactttttgg
    77701 tgactggttg agtggaaaat gtaggtacat ttcaatgcga aaatgtatta taaccccaaa
    77761 cgaggtcaat aaaaatgact caaacttgca atataaccct aaacgggtag gggatttatg
    77821 aaaaattgaa tttgccggcg taggagctgg ctgttttaag tatgaatgaa cacagctttc
    77881 gggctgtttt atataaatag caggttactg ttcacaaaca gcattttgtt aagaaagtag
    77941 tgaccacagc agtggtggaa agaggaaata ttatgaagaa acagtataac attgcagtcg
    78001 ccggcaccgg ttatgttggt ttgagtattg ccactctgtt ggcacagcac cataaggtta
    78061 cagcggtgga tatcattcca gagaaggttg aactgattaa taaccgtaaa tctccaatcc
    78121 aggatgatta tattgaaaag tacttggctg agaaagaact gaatctgacc gcaacattgg
    78181 atgcaaaggc agcataccag tctgcggatt ttgttgttat tgcggctcct actaactaca
    78241 atagccgcac tcaacatttt gacacatccg ctgttgaggc tgttattagg ctggtcatgg
    78301 aagtaaatcc tgatgccatt atggttatta agtccaccat tcctgttgga tacaccgctt
    78361 ctgttcgtgt aaagactggt agcaagaata ttattttcag ccctgagttc ctgcgtgaaa
    78421 gtaaggcact gtatgacaat ctgtatcctt cgcgtattat tgtgggtacc gatatggatg
    78481 atgagcgttt ggttgaggat gcacacactt ttgctacact gctacaggaa ggcgcaatca
    78541 aagagaatgt ggacacattg ttcatgggct ttactgaagc agaagctgtt aagctgtttg
    78601 ccaatacata tctggctctt cgtgtcagct acttcaatga gctggacact tatgcagaga
    78661 tgaaaggctt gaacactcag cagatcatta atggagtttg ccttgatcct cgtattggta
    78721 ctcattacaa caacccttcc tttggttacg gtggctattg cctgccgaag gataccaagc
    78781 agcttctggc caactacgca gatgttcctg aaaatctgat cgaggcaatc gttgaatcta
    78841 accgtactcg taaggacttt attgcggatc gtgtacttca gatggctggt tattacaatt
    78901 acgcagaagc aaatcagtat gatgctggca aagaaaagcc tgttgtgatt ggtgtttacc
    78961 gtttgaccat gaaatccaat tctgataact tccgccagag ttctatccag ggcgttatga
    79021 agcgtgtaaa agctaaaggt gcaactgttg tgatttatga gccgaccttg aaagatggag
    79081 agactttctt tggcagtaag gttgtgaatg atttggaaca gtttaaggca atgtgtgatt
    79141 ctgttatagc aaatcgctac gatgcttgcc ttgatgacat taaggaaaaa gtatataccc
    79201 gtgatatctt ccagagagac taagattagg agagagtatt tatgattatt acaaaaacac
    79261 cgtttcgtat gtcttttttt ggcggtggaa cggatatgga ggattatttc aaagagaatg
    79321 gcggtgcagt tctttctact acttttgata agtactgtta tgtgaatgtg agacatctgc
    79381 ctagattttt tgattacagc gctgagttat cttattctcg tactgagaga gtaactagca
    79441 ttgaagatat tgagcatcct gcgattcgta atgcgatgaa aatgctcgat atgcatgaaa
    79501 ttcggcttac atacgaagct gatttacctg ctcgctctgg tctgggtaca tccagttctt
    79561 ttgcagttgg tatgttgaat gctttttatg cactggaggg taagtatgct gacaagaaga
    79621 aacttgctga tgaagccatt tatcttgaaa gagttctgtg taatgaagcc ggtggatggc
    79681 aggatcagat tgccgcatct tatggtgggt tcaatcgaat taatttcaat gtggatggtt
    79741 atgaggtgct gcctgtcatt atttcgcctg aacgtaaaaa gcagcttaac aacaatctga
    79801 tgatgttctt taccggattc actagatttt cttctgatgt tcaaaaggct aacaacgtca
    79861 gtggcacaga ggataagaga gtaagactga aaaagatgta tgagcttgta gatgaagctg
    79921 aggcaattct tactgataag aacagaaatt tagatgattt cggtagacag cttgatgtta
    79981 cttggagatt gaaaaaaggt actggtggag caattagtac aggaagcatt gatgaactat
    80041 atgaaaaggg tatggctgca ggtgctttag gcggaaagtt gctcggtgca ggtggtggag
    80101 gtttcttggt tttctatgta caacctgaga aacaggatgc agttcgttgg gctatgcgtg
    80161 atttgatgta tattccgttt gaatttgaaa atggtggaac tagagttatt cactacacgc
    80221 cggaaactta tattgaaaaa cagaatggat aaggagtaac aatgttagcg gaaaaactta
    80281 ataaacatat tgacttattg attaaacgat atcctacact tgagacgatt aaacaggaca
    80341 ttatcgatgg atacctaatt atggaggaat gctatttgaa tggtggtaaa cttcttgtgg
    80401 caggaaacgg aggatcagcc gctgactcgg aacatattgc tggcgagttg atgaagcggt
    80461 ttaagacgcc acgtcctgta agtaaggaat ttgccgataa gttagttgca attgaccctg
    80521 taagaggtcc ggaattagcc aaaaatctcg agtgtggttt aacggcaatt cctttggtcg
    80581 cacacgaagc attgtctact gcttatatta atgatgtcga tggtttgggt gtcttcgcac
    80641 agcagctgtt tggctttggt agatctggtg acgtattcct tggtatttcg acgtccggaa
    80701 atagcaagaa tattatgagc gcaactgttg ttgctagggc tctcggtata aaagttattg
    80761 gcttgacggg cgcaaagggt ggggaacttg ccacggtagc agatgtttgt gttaaggctc
    80821 ctgaagttga aacatacatg attcaagagt atcacttgcc gatatatcat tgttggtgtt
    80881 tgatgctgga agataaattc tttggaaacg aataaaattt ggaggaatgt taaatgaaaa
    80941 agacaattat ggttaccggt ggttgcggta tgattggctc caatcttgtt aagagactgg
    81001 tgaaggaagg ctgctgggat gtttatgtag ctgataatct gtggagagga aagttggagt
    81061 acctgaacga cgaagatggg catcctgtca tcgacctgga tactcatttc ttcaatgctg
    81121 atctgactga ttatgagcag gctaaaaagg ttattggtac gactgagtat gtagttcatc
    81181 tggctgatgt tgtggctggc attgactatg tgtttaagaa ccagggcgaa ttgttccgaa
    81241 tcaataatct gatcaattct aatgtttttg attgttgccg taaggctggc aaggagaaaa
    81301 tcaagggtgt tatctatgtt ggtaccgtat gctccttccc tctgaccaga cagaatactt
    81361 tgaatcctga gcctctccgt gaagaggagt tgttccctgc tcttcctgag tctgcttatg
    81421 gctggagcaa actgatgggc cagttggaga tggggtattt ggaaaaagaa accggaattc
    81481 cgtgctgtat cctgatgctc cataatgttt atggcacacc tactgatttt ggcgaacgta
    81541 gtcaggttat tcctgctctg attcgtaaag caatcaataa tcctgaagag ccttttaatg
    81601 tgtggggtag cggagagcag ggacgtgctt tcattcatgt caatgatatc gtaaatgcat
    81661 tggtgctcgc cctggataag ggttggggtc atggtcatat tcagattggc ccttctcact
    81721 gtacttccat caaggaaatt gcatataaga tcattgaaat ttctggcaaa gacatcaagc
    81781 ctttctttga tacttctaag cctgaaggtg ataaggctcg ttgcgcagat tattctaagg
    81841 cgaaagagat tttggattgg gagcctactg tttctttgga agagggtctt cgtgaatcct
    81901 acaattggat tgaaaagcag attaagggtt aataagtata taagctgttc ttcctccgtg
    81961 taattagagg atgcggagga agaacatatg agtaaggatg tactgagatg gaaaagaata
    82021 tgtataaaag aaaacgtgat ttcactgtgt gtggaatagt aggtgtttgt gctactacaa
    82081 agaacgagtt tgtaagggaa gttttttcca tgccaacatc tccggtaagt gcgactgttg
    82141 atctcgtggg agtgccggcc attatttcgg caaaggaaaa tcccaaaatt gctgaaatgt
    82201 atagccaggc tacaatggct gcaatagatg gtatgcctat tgttaaaatt ggacggcgca
    82261 aaggatttac ctgtgaacga tgtgctgcac cagatattat gggaatggtt tttgaagaag
    82321 gcgttagaca gggaaaaaca cattatttct atggtggaaa agataacgaa gtgttaaata
    82381 agcttcgtgc aaacctagaa agagattacc cgggaatcaa aattgttgga atgtattccc
    82441 caccatttcg tccgttaaat gaagaagagg atagcaagat ttgcgaggaa atcaacaact
    82501 taaatccaga ttttctgtgg gttggtattg gtgcgcctaa gcaagaaatg tggatgtggg
    82561 aacaccagaa gaagatccat aacacagtaa tgttcggtgt aggcgccggc tttaactttt
    82621 ttgcgggcac tttggaaaaa gctcctgaat ggatggaaaa agctagtcta gagtggttat
    82681 ttagactggt aaaggaacct cgtagactat ggaggcggta tatcttggga ggtataaagt
    82741 atatctacta tactattgaa tctaagtagg ggggcaataa aaatagatga aaatacttat
    82801 agtagttaca atgacgaata actttggtaa gaaggggtcc tataattctc aggaaatagg
    82861 attagctcga gggttagctg aattaggcca cgaagttatt gtatataaaa gtgtaaaaag
    82921 tgatgaggaa gttggaacag agcaattaag caatacggcg atgattcact atatcaagtg
    82981 tcgttatgta ggatcaaatg gattcttatc aacaaagaat tttgataaga atgcggatgt
    83041 tctaatacaa tttgcggacc ttcaactatc agtacctcat gtatactcat ggactgtaaa
    83101 aaatggaata aaatatatac cttataatgg gataacgcga agtcaaagtg gcaataaata
    83161 tataaagaca attattgaaa tattggcgca tcgaaattat aaaatttata agaaaaatgg
    83221 gtgtgttgcc aaaaatgatc gagtttctga aaaattagta caattaggag taaataaagt
    83281 gaaacttgct cctgtttgtg tggattttga acagttacat aagaattacg aagcaataga
    83341 ttttactact ctcagagaaa aatggaatat tcctcgcaat gttcgtgtta ttttatatgt
    83401 aggacggttt gaaaaggata aaagaccgtt agatatgatt agtttattta ataaacttga
    83461 tgagcataat tatttgctga tggtaggaac tggtgcttta ctcgagcaaa cgaagcagca
    83521 gataaattta cttggactgc agaatagagt aaaaataatt gagcgtattc ctaacaaaga
    83581 tatttgggag ttatattgct tatcggatgt atttgtaaat ctaaactata ctgaaatttg
    83641 gggtatggtt ttgctagagg caatgtacta tcgcctcggg attattgctg ttaatgcacc
    83701 tggcccacag aatattattt tggataatag atatggaatg ttggctaaat ctgacgatga
    83761 aattttgaca ttcttaaaag acggacgatt taccaacgat atcatagatg cagcatatca
    83821 gcgtgtaact acagaattta gatggtcaaa gtcgggagaa attatgctta gcatgttgga
    83881 aggtgataaa tgatatggaa gtaacgaaat taatggcatt gaggaatcga tatgctttaa
    83941 acatagttga taattgcacg agaaaaatag ccaaaattct tggttgttgt attggtaaag
    84001 gtgcacagat agggaactct gttgaatttg ttcataattc tgtaggcact gtgattcatt
    84061 ctgacacaat ccttgaagat ggtgttaaag tctatcagaa tgtgacgtgt ggttgagcag
    84121 atagtaatga acgtatcatc attcgagtaa aaaagaacgc tgttttatgt gtgggtgcta
    84181 aaattctttg taaatctaac acagaattat gcgttgggga aggtgctgta gttgctgcaa
    84241 atacagtggt attgaggtct attccaccct acgaagtgtg gggggtacct gctaaaaaga
    84301 tggggagata aaacatgaat gcatataaat tatacaaagt agaacgatgg ttttacttac
    84361 acaaaatgga gttttttgcc aagattgtaa aggcactaat ttatgtactt cataatagct
    84421 caatttcata tcaaacagaa attggagata attgtaagtt cttatacggt ggcataggtt
    84481 gtgtaattgg aaaagataca aaaataggta atcacgttat aatcggaacg aatgtactga
    84541 ctggtggtag atccaataag cctggtatgc ctacaattgg aaacaatgta tatatcgcta
    84601 ctggcgcaaa aattttaggt aatatcacaa taggggacaa tgtaatagta ggggctaatg
    84661 ctgtggtcat taatgatgtt gaacctaatt gttcggtagg aggcgttccg gctagagtat
    84721 tgaaaaaaga tattgatgta aatgattatt gtagtttgaa tcgttactaa aaggagagat
    84781 tgtatgttgt attttgttat tcctttaatg agtgcgaagg cagcaaacaa ttgggagatg
    84841 gttagtacct tatttaaccg aacattatgg tcgtgttata atcagactga tcctgatttc
    84901 aaaatcattg ttgcttgcca tgaaattcct aaactcacgc atgaatatga tgacagagtt
    84961 gaatttattc aagttagcga aaaggatgcc cctattccca caaatcaaga cgaaaaaatg
    85021 attgataagg gttataaaac acatgctctc gcaatgcgtt tgcgagagtt gggcggcggt
    85081 ttcgctatga tggttgacgc agatgatctt gtaagttctc atttggcgga atatgtgaat
    85141 tcccatcccc aggaaaatgg attttatgtc aaaacgggat acgtgtactt tgttggagac
    85201 gattatatga aggttctacc aaagttctca tcaggaagtg cgtgtatagt aaaatatgca
    85261 gtggaagacc tgcctgaggc atatccggaa gttatgacag caaattgtga tgataatcca
    85321 tggatcatta gaaaacgaca tgggggcgtt gttccagcat gtgaagagca agggcgtcca
    85381 ttaaaacctc ttccattcaa agcagcagta tatgtattag gaacagggga caatcactcg
    85441 ctttatggta aaacgacaaa atatcagacg aaaatgcgag agatactgga aatgtttata
    85501 gcaaaacgta aaattaaagg aaaactgaaa gcagaatttt ctgttgattg gttataaggt
    85561 gagtattatg aaggtattga tgtgtgaaag ccaaatgcca gagaattggt attcgtttga
    85621 tctttctaaa gaacttaatc aatatgttga tttaacattg ctgtgtaagc gtgatgttcc
    85681 tcctttagat cctaaaatta agagcaaaca tattttgtat cataaaagta caaataaagt
    85741 gttttcggca cttgtttttc tgaaaagctt gttcttaatt tggaaggaaa taagatttgg
    85801 tgattatgat attgttcatt tgcaattgat gaaaagtctc aaatatgaag gcggattgta
    85861 cgttttatgt aagagacact ataaaaaatt aattatgaca gttcataatg tgttaccaca
    85921 tgaggttcgt gaaaaggatc gccaagagca ctttaatata tattcaaatt gtgatggttt
    85981 cattgttcac aatcgagtaa cagaacagca tttatatgct gaattcccag aattaaatgc
    86041 aaagtcatat gtttgtccac atgctatcta tggagatgtt aaacatatat ctagagcgta
    86101 tgataaagat aagtttgaat ttatcttgtt tggacaaatt cgtccatata agggaattga
    86161 tgtactgctt aatgcaatca cagaacttcc aatggatgta agaaagaaag ttcattttac
    86221 catagcaggt caacaacatc ctaaacagga tagtactgac tataaaaaaa tgattgaaga
    86281 tttaaaggtt tcagattgtg ttctatataa gccagagaga atttctgatg aagaaatgga
    86341 cagtatgttc agaaatgcag atgccgcaat atttccatat aaagaaatct atggaagtgg
    86401 agcgcttctt atggcataca cttattgctt accaactatt gttagtaatg tacctgcttt
    86461 tatggaagaa accgaggaag gaaagatagg gttacttttc gaaaatttga attctaagag
    86521 cctagcagac acaattgtaa agtatgtgaa ccttagccca gctgagaaaa gcaaaataga
    86581 ggatgatata tatgagcttg taaaccgcaa gtatacctgg gcggcatctg caaaactcac
    86641 atacgaggca tacaaacaaa ttttgaatga gtagtaaaac aattagaaag gaataataat
    86701 taacatgcag attagtaaat ggaaaatcat acagtttcta atggtctttc taatgattat
    86761 ctcatcaagc atattgaagc ctttgggcgg ctggttggga gaaacgatga acgttagact
    86821 tgtgacagtc attctagcaa tagtatgttt gttaatagcc tcattaccag catttagaaa
    86881 aaatagatat ttaaatttct gcactttact gatttgtgtt gttataatag cagcttacat
    86941 ctactcagat aagacaatat gggttaaatc tagggaaagt atgagtttat actatgtatt
    87001 attggcgtat ccattgttct gtactttaat taaccaagcg tggaatttgg attcaatgct
    87061 aaatacgata tgtggtgtaa catttctttc atacattctt agaacatcta tttcacttat
    87121 tcagaaattt tcaggagtac ttctctatga aaacatatat cgtgagtgtg ccccggaaaa
    87181 ttggtatcgt ggaggctttt taagaattaa tccaccatgt tttacaatta taataatacc
    87241 gattgctttg tatatgattg agcgacaagt tgtagctaga agaaaagtaa aatattattt
    87301 actgattgct agcgcactgg catattctgc tgttattcat ggttctagat ctgtccttgt
    87361 ctatcaaatt attgtacttg gttgctatat cctttttaag aaaggttctt cgaaaagaaa
    87421 aattgcaatg tgggttttag ctgggttgct tgtagtcata tttattaatt cgaccatgtt
    87481 ctcaacattt gttgaaactt tcacaaacag tacaggtgaa tatgcaggat ctgctgaatc
    87541 tcgatttgct tcagtttggt tttttattcc taaatttttg caatcgcctt tgttcggtac
    87601 tggtattctt actggtgagg aatttacgtt tatattacca ggaggagtta gaggacacct
    87661 atctgatatt ggatttttct atacaattac tcaatatggt gtaggtatac ttatatttga
    87721 tattctgttt attataaaag ggttcaaaac agcattactt gcatcaaaag tgggtatgac
    87781 tggctatcaa tatttagcat ttggaattac attaaatgtc ttgctaacag ggattaatat
    87841 agactggttt tatccgatat ttactccagc tattcctgta tgcttagcgg ttatagaata
    87901 catctatcaa gaatgtagaa gtgtggctaa catagaatag gaaatattaa taggagtgca
    87961 tctcatgaat aagaaagaga ttttatataa catattgaag aagtacgaaa atttaccata
    88021 tgtgtttagg aacatgtttg gtcataatat aagtaaactc gtatcttgga aattgactgc
    88081 tgattgtgca acagtagaag aaaattctga agtttatgca gcgtttcaat tgcgctcaaa
    88141 ggacatgtct gatgttccag tgatgggttt agttaattgt tgcaaagatg tagcaattgt
    88201 aatgcagggg ccactatgtt taaaggatga tttctcttat aatactctgg cctactataa
    88261 gaaatgttat ccaaatgctt taattattct ttcgacatgg aaggacgaag atattaaggc
    88321 attgaaaagg atagaggatc ttggagttat agttatttgt agtgagaaac ctgaaaagcc
    88381 tggacactta aatattaatt atcaagttgg aaacactttg cctggaatag tcagagcaaa
    88441 gcagcttggt gcaaagtatg tttgtaaaac tagaacagat caaagaatat accatccaaa
    88501 tgcaatggcg tttttctgca gtttacttga acagtatcct gttaataacg aagatgattg
    88561 gaaattaata caaaatcagc gattactgtt actatcaatg ccgtatggag acatgttttt
    88621 cccgtattgt ttgtcagact tcctgtattt tggtgatgtt gatgatatga taaacctctt
    88681 ctcgattcct agtgatgtga gagaaaaggg agctgtgtct agaggagtct caagaaggga
    88741 gatatctgaa aataatttag ctccagaagt tcagcttctt cgaagctata tttcacgaat
    88801 ggggggcaat gaggagtgct ctattagggc atattgggag tttgtaaaaa accatgttat
    88861 tactattaac aaaaatcaaa ttgatttgta ctggcataaa tatgtaggtc gttattcaaa
    88921 taatactatt tatggtacgt attatattga tgatagccaa gatgcacttc actgctataa
    88981 ttttgatttt ataaattggt tgaatttgta cactggaaaa tacgaataca aagcagaata
    89041 tgaaaaatat atggactttg tttgggaaga gtaattaact tttatagtaa aaagtacttt
    89101 agggaagggc atattatgaa aacagtaatt atggcgggcg gacgaggaag ccgaatttcg
    89161 gaattgtttc cggatattcc taagccaatg attcctatag atggcattcc agttttagaa
    89221 cgagaattgt gttcgcttag agatcaaggc tttaatgata ttattttgac tgtttcctat
    89281 ttgcatgaaa aaatagagga gcatttcgga gatggttcaa aattaggaat taaaatcaaa
    89341 tattttattg aaaaaactcc acttggcaat gcaggtgcat tgtatttttt gttggatgaa
    89401 ctgggtgatg aaccgttttt acttttgaat gctgatgcag tatttgatgt tgatttcagg
    89461 cagatgatta gatttcatgt tgagaataac gcagatgtta ctttgtttac gcatcctaac
    89521 tcacatccgt atgatagtgg attaattata tccgatgaca ataagaaagt gcttcaatgg
    89581 ttagcgaagg aagatcaaag acctgaatat tataaaaacc gagtaaatgc aggtcttcat
    89641 gttataaatc cgaaggtgtt gagagaaaat ccattaatgg aaattgtaaa taaatcaata
    89701 gttgaaggtg gggggctacc atacaaaatt gatttagata gaatgattct gaaaccattg
    89761 gctaacacag ggcgtatgta ttgctacgat tctccagagt atgttaaaga tatgggaaca
    89821 ccggaccgat atagagctgt aattaaggac tttcaaaagg gaattattgt tggtaaaaat
    89881 ctatccaatc cacaaaaagc aatcttttta gatagagatg gaacgattaa taaatacgtt
    89941 ggttttttga gaaatgcaga agaatttgag ttaattgatg gagtatcgga ggcaattaaa
    90001 attattaatt ctctaggttt tctgtgtatt gttataacaa atcagcctgt aattgcaaga
    90061 ggtgaagtta ctgttgatga gcttaatagt attcacaata agcttgaaac aatgctaggg
    90121 catgctggtg gatttttaaa cggtatttat tactgtccgc accatcccca taaaggatat
    90181 gcaggagagg ttatagaatt aaaaattgat tgcaattgtc gcaagccaaa acctggactg
    90241 ttattagaag catcaaagga ttttaacata aacctaagtc aatcatttat ggttggagat
    90301 agtgaaagtg atgtaattgc tggaaaaaat gctggttgta agacagtact gattacagaa
    90361 gacatcacct tgcctgctaa ttatggccaa gactatagtg tctgttcatt gttagaattt
    90421 gttaaaaatc atttatgcac tatgcaataa ttggtttagt gaaacttgta aaaggagtaa
    90481 ttagatgaag gtattaattc cagcagcaaa agttgttgaa aaagaattgc agagtatagg
    90541 aatgttacca gcaattatct atccaattaa taaaaaaatt gtctttgatt atttgtataa
    90601 tcagtataat gcaatctgcg attcattgga tattatctgt tatgaaaaag cggataaggt
    90661 tcatagaaga ctgaaaaact atcatgattc cagaattcat atatgtgacc ttcctgaatt
    90721 aggggatcta gggcaaaccg tgtattttgg gttatcagat gaagatgagc ctgttctaat
    90781 taacttcgca gatacaattg ttatggagag tatgtctgat atagaaatgg atacattcta
    90841 ttattctgaa gattttgtgt cgagtacttg gacatacttt aaaattaatg aaggaataat
    90901 tactgatatc cacgataaag aggaagctta cgtatccgat aaacgtgaaa aattatttat
    90961 tggcgtattt atgttttctt cgtcaaaact tctgaagagt tgcttaagag aggcagttga
    91021 gagtcggaaa caggggggaa taaactcatt ttatcgggct ttacagctat acagcgaacg
    91081 ttgtccgcta aaggccgtaa gaacagaaaa gtggtttgat attggacaca tcgataaata
    91141 ttacaataca aagttggaag ttaaagcacg agagtttaat catatctcta tagataaaga
    91201 tcgagggata ttgaagaaaa ctagcgatga caaggataaa tttattggag aaataaagtg
    91261 gtatttaaaa cttcctgctg atattgaata tgtaagacct agaatttttg attattctac
    91321 atcttacgta gctccctatg ttgatatgga atattatgca tatcatacgg ttcatgagtt
    91381 atacttatat ggtgatttaa aatataacca gtggcatgat ctgtttagcc gtataaagtt
    91441 tatctgtagc gattttaagc gatacacctt aaaagatgaa aagattagag agtcattgga
    91501 agatatgtat ttgcgaaaaa ccatgcagag atttcaaaaa ttgtctgaag ataaaagatt
    91561 ttcagctttt tttgagaaac aaattcaaat taatggtcgt aagtttattt ctttaaccgg
    91621 tgtaattgaa cttctaaaga atcttatccc aagtatgctc tatgatgtaa atgaattttg
    91681 tattatacat ggagatttgt gttttgctaa tatgatggta gattccaatt gttcctttgt
    91741 taaggttatt gatcctaggg gaaaatttgg aaaatatgat atttatgggg acagcagata
    91801 tgaattagca aaactatttc atagtttaga tggtaaatat gattatatca tcaaggatct
    91861 atttactgta gaatatgata ccgagtgtgc agtgatagat tatacaatta atacgcgtgt
    91921 aagagattac gatttatttg aattgtttaa gtcggtcttt tcggacgaga ttaaggacga
    91981 ttttgataag attgtattga tagaagcgtt gctatttctt agtatgattc cattacacgg
    92041 tgaaagtttt gatcaccaaa tagtgatgct tgctacaggt atggaacttc ttgcaagagt
    92101 aagtaataaa gttatactct aatataaatt gtgccttaat gaataacggt tacaatactg
    92161 ttttagcaat gtaggcatta aggcacgatt atattaatta atacaataga agggaagaga
    92221 aaaatgtata gtgaatattc ttttgtgttt gatatcgatg gcactttgtg tccgataaag
    92281 aagaaggatg agcgatatga ggatttggtg ccgtatgaga atatggttga aaaactgaga
    92341 ttctataagg agaatggtgc taaaattaca ttgttcacct cacgcaatgt taattcctat
    92401 aaaggaaatt tgggaatgat aaatgctaat actgcaaaag tgctcttggc gtggttggat
    92461 aaatgggaca ttccgtatga tgaaatcctg tatggtaagc cttggccggg gcataaaggc
    92521 ttctatgtag atgatcgaac ggttagacct gatgagtttt tgagatgctc tgtagatgaa
    92581 ttaaatgaaa tctgcaacag ttcaaaaaaa agattctaaa taaggaggtt agttctatgg
    92641 aaaaaaagat gacagttatc attacgatgg caggtttggg aagtcgtttt cgaaaaattg
    92701 gttatgcggt tcctaaatat atgattaatg ccaaaggaag aactttattt gattgggcca
    92761 tggatagttt ggttggatac aataaaaatg ttgataagta tatttttgtt gttcgtcagg
    92821 aagacaacgc ttcagctttt ataaaggagc attgtgctta ctttggtatt gaaaacgtag
    92881 aagtgattga aatagactat atgacagatg gtcaagcaac tacatgtatg ttggctattc
    92941 cttattgcaa tcaagacaat gagattatgg tttacaatat tgatacatac gtggaatcaa
    93001 atgagatgaa atacgaagat atttgtggag atggacatat tccatgcttt catgctgatg
    93061 gcgatcattg gagttttgca aagcttaacg atcgaaatga agtaatagaa gtccgcgaaa
    93121 aagttcgcat ttctgataat tgtactcttg gtgcgtatta tttctcatcg gcaaagctgt
    93181 ataaagcttt atatgaagag tattatgcag actcctctaa tatggagaag aatgaaaaat
    93241 atatagcacc attatataac tttatgataa aaaaagggtt aaaggtgact attagcatag
    93301 tagatgcgaa aaaggttcat gtgcttggta ctccagaaga gctcaatgtg tttttgaatc
    93361 aagaataaat agtgtttagg agaaaatagc agtgaccagt aataatctaa ttgtaaaaac
    93421 attaagcaga tataagaata ctccacttcc tgttaaggct gcattatggt ttatggtatg
    93481 tggtttttta cagagagggg tttcaatgat tacaacccca atttttacta gagttttgtc
    93541 tacagaacaa tacggtttat acagcactta tctatcgtgg gaatccttat tgctcatgat
    93601 tacatcgctt agtttgttca aagccatgat gaatctttat gtaaaatatg ataatcaaga
    93661 acatattctt tcgacagtat ccggattgac tttagtactg actatttttt ggtttgttat
    93721 tgctattttt attaaggata tattggcaga aattttagga ttgacaccag ttctagttgt
    93781 ctgtatgttc tcatatttta tatttcaggc agaaatacaa tgttggtctt tacacaaacg
    93841 atatatatat gattataaga ctttattagt agctaccctt gcagttactg ttggctcatc
    93901 tgtagttggc tgtttagcgg ttctgtttat atctcctacc gctgaggcga gagccttaat
    93961 gaatgtccta gtgactggtg ttgttggcgt ggctgtgtat atatcggtat ttagaagatg
    94021 taaggatttt aaggataatg aagtgtggaa tttcgcagta gtattttgtg tatcgttgat
    94081 gccacactat ttatctgaat ttattctgaa tggttctgat aaaattatga taaattactt
    94141 atgtggttcg tcagatgttg ctatttacag cgtggcatat tctattggtg ctctaattaa
    94201 ccttgttaca aattctgtca attccacttt tgcaccttat cagtatcaaa aaatcaagtc
    94261 aggagaatat gatttactat caaaaagagc taatgaagtt cttggtttgg tagcggctat
    94321 gctttttgca ttaatgctat ttagtaaaga aattgtgttg atatttggtg ggcagaaata
    94381 tatcgacagt gttaatttaa ttgtacctat atgtatcggt atttatttca actatatgtt
    94441 tcagctgttt gctagagtac aggaatactt tgaaaagaaa ctcactgtcg ttattccatc
    94501 ggttttgtgt gcaattatga atattgtact aaattatgtt tttataaagt tatatgggta
    94561 tcaggcggta gcatacacaa ctgtattctc atatatgttt ttctgcgtca tccattatat
    94621 tttttataga aaagtttgca aagaactatt ggatggaaaa aacatatatg atgtcagagg
    94681 aatacttttg atttctgcaa tcacgatagc tggtggcatc ctaatttcaa taattaataa
    94741 atttgatttc ataaaatatt taattatagc aattgcttgt gtcgctgcaa ttatatttag
    94801 aaaacgattg ctacttttag taaggagaat tatgagcaaa taaacatttg ctcatagaga
    94861 gatagagaat gatagatatt atttctgcta taacggtaaa tgtattaaca gcattatatc
    94921 agccgttctt atttgccgta ttaacagcgt atctgtttat gtactggtat ctatttatag
    94981 ttgataactc tcgagatcct aaaaacttaa aagattctgt attaatctgg ttgagatatt
    95041 ttaaagcatc tagtaaattt cgcagactct tttttcttgc gttttatttt actttgatat
    95101 tatttaaaac cttgcttaat cgaaatatgt ggatgaaccc gctttcaaac gttattggac
    95161 agtggtcatt atatattata gataatgcta caggagagag cagattgaca acagaatgcg
    95221 tggagaacac gatgctgttt atcccttttt gcatgttatt gttatggtgg cgtgacatta
    95281 aatgtgatat tatacgcacc atctatgttg ggattaagta tgtgttttta ttctcattaa
    95341 gtatagaatt tacacaactg ttttttaggc ttggtacatt tcaactatcg gatttgtttt
    95401 ataacaccct agggggctta attggtgctt tgctatattg gctattctac cgattaaata
    95461 agtatataga cttaaaatag aactgctttg aattttaata tggttaatat ttttctgcag
    95521 acaggattgt gtaatctcat caagaatact ttacatgcca tctatttttg ctaaaacatt
    95581 gttgttatat cttcaagagg tagggaattt tattgcttta aatgaggtcg agcgaattaa
    95641 aaaattggta aaatatataa catggtggta gttatggtaa aagtgtttga tataattaag
    95701 ttttcggatt tcagaggata tactgctgtt gcattggata atgagactct tttctcgatt
    95761 ggtattgagt ttcacattaa gcaaataaat caaggattta gtcataataa atatactatg
    95821 cgagggctac attatcagag tggagagcat gctcaaggaa agatagtgat gtgcctacat
    95881 ggtgaaatta tgaatatcgc acttgatatt cgacaaggat cttccactta tggatgtgtg
    95941 tatgtcaaca cattgagtga aagcaatggt aaatgtgcgt atatcccacg tggatatgct
    96001 catggctatc ttactatgga ggacaatacg ctagttcagt ggtgcgtaga taatgatttc
    96061 aattctaatg ctgccaaatg tataagttat gaaagcgtag atattgaatg gccggtcagt
    96121 cggcagtgtt tcgttataag tgagaaagac aaaaatgtgg aaagattaag cttttgatta
    96181 aaatgagctg tggagaaaag aaggatactg gaaaataaga agaacaataa ccgagcaaat
    96241 gctcggttat ttctatgagg aatacataaa actaaattta gatatagata tacccgtggt
    96301 gctagttaat tttaatatcc taaagaatat ctcaaagttc cttcaaaacg tcgttgtgct
    96361 cgacttaata gaacactgta agccagtaat atgctttcaa atctactctc tagcccttta
    96421 acagaacgtg agttgaaatt ttgacaacct aatttttcta aggaagaaaa aacagtctca
    96481 actgttttt
//
LOCUS       DACEEZ010000004        83001 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20313_2017
            SAMN12639966-rid9879223.denovo.004, whole genome shotgun sequence.
ACCESSION   DACEEZ010000004 DACEEZ010000000
VERSION     DACEEZ010000004.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639966
            Sequence Read Archive: SRR10023158
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 83001)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 83001)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741683.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741683.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:44:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,016
            CDSs (total)                      :: 2,962
            Genes (coding)                    :: 2,889
            CDSs (with protein)               :: 2,889
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 36 of 73
            Pseudo Genes (incomplete)         :: 29 of 73
            Pseudo Genes (internal stop)      :: 18 of 73
            Pseudo Genes (multiple problems)  :: 8 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..83001
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639966-rid9879223.denovo.004"
                     /isolate="E20313_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(<1..563)
                     /locus_tag="IWO89_000289"
     CDS             complement(<1..563)
                     /locus_tag="IWO89_000289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF443 family protein"
                     /protein_id="HAQ0455077.1"
                     /translation="METVRLWIFMKNFRSALRYVILESKSGYYLIDTDHYTFWGGYLF
                     PIFNWIIPHKVMKINLPAEEINRLILNKDLQRKREKEHSNVAWIGPAFAVVTSSIVGP
                     MLESIYSDFSIGIRYLFVTLSIAVLLCIKGYVSKKNKDILTLIGVNNLSSTKIILFPE
                     SIKEGSRLLIIFIMLILFSVLFGVMFI"
     gene            complement(750..1400)
                     /locus_tag="IWO89_000290"
     CDS             complement(750..1400)
                     /locus_tag="IWO89_000290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0455078.1"
                     /translation="MFIKGGQKMCFFTTFTIIMIAVLFIFLDIAKRNTAFLLYRVLLR
                     AGLITFISIVGFFLFTVIVFIWRTTAPPLPEITYGEFPFSLEYEIDEERILIEDTVIA
                     EFIGSFRGNVTTQPRRSWSIKLLNNSGELKAFHYEFLIIESEDVTITLSSGSAGYFMN
                     DGGSKPQLTVRDRIAGFMHFSHYDLEEARKILAEYGITLINWEYAPPIRNFFNDYY"
     gene            complement(1943..2878)
                     /gene="whiA"
                     /locus_tag="IWO89_000291"
     CDS             complement(1943..2878)
                     /gene="whiA"
                     /locus_tag="IWO89_000291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein WhiA"
                     /protein_id="HAQ0455079.1"
                     /translation="MSFAADVKKELTGLEVHREHAKAELAALLRMNGSLGIVNQQFIL
                     NVQTENAAIARRIYSLLKDHYQVRSELLVRKKMKLKKNNVYIVRLKQGTKDVLMDLDI
                     MDGMMFNGHVSDEIMGNSQKMRSYLRGAFMASGSVNNPETSRYHLEIFSIYEQHNQDI
                     CDMLNYYGLHARTLERRNGYISYLKGAEKIADFLTLIGATNSMLRFEDVRIVRDMRNS
                     VNRLVNCETANLNKTIDAASKQIENIELIEAKVGLHALPEKLQEIAELRLQHPEVSLK
                     ELGEMIPSGAISKSGINHRIRKINEFAEKLREEAV"
     gene            complement(2912..3910)
                     /locus_tag="IWO89_000292"
     CDS             complement(2912..3910)
                     /locus_tag="IWO89_000292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YvcK family protein"
                     /protein_id="HAQ0455080.1"
                     /translation="MKMKTYRIRKPKIVVVGGGTGLPVILKSLRNQGADITAVVTVAD
                     DGGSSGAIRESIAMAPPGDLRNVLVALSDMPQFYEDIFQYRFKKEDQFLANHTIGNLI
                     IAAVSEMRGSTYEAIQLLAKMMHVDGHVYPSSETPLTLHAVFKDGTTAVGESKIAINR
                     KTIDRVYVCNTNDKSEAKAARKVVSAIMDADMVVLGPGSLFTSILPNLVIPEIGEAML
                     KTTAETVYICNIMTQKGETEHFTDADHIRVLHKHLNQPFIDTVLVNTEKVPEDYMDPE
                     IYDEYLVQVAHDFNGLRNEGCRVISTDFLELKDGGVFHDGDKVVEELFRLVFGSKY"
     gene            complement(3907..4791)
                     /gene="rapZ"
                     /locus_tag="IWO89_000293"
     CDS             complement(3907..4791)
                     /gene="rapZ"
                     /locus_tag="IWO89_000293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNase adapter RapZ"
                     /protein_id="HAQ0455081.1"
                     /translation="MTDNLQLVIITGMSGAGKTVAIQSFEDMGYFCIDNMPPRLIPKF
                     WELIKESGKVTKMALGVDLRSRSFFEEIQNMLIEIENTNFIDTSILFLDASDEELVAR
                     YKETRRAHPMAMDGLITEGIRKERAILDDLKAQASVIIDTTTLTPRQLREKINESFKS
                     KGDQGFRIEFVSFGFKYGLPIDSDIVMDVRFLPNPHYIPELRPLTGKDQPVYDYVMSF
                     DETQSFYHKFLDLLETVLPGYIKEGKSSLTVAIGCTGGQHRSVALTERLANALGKNYK
                     VNVTHRDKDKRKETVNRS"
     gene            complement(4926..5657)
                     /locus_tag="IWO89_000294"
     CDS             complement(4926..5657)
                     /locus_tag="IWO89_000294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate racemase"
                     /protein_id="HAQ0455082.1"
                     /translation="MENFFSILGGMGTMATESFVRLINHRTKATKDQEYLNYVLFNHA
                     TVPDRTAYILDRSEENPMPFLLDDIEKQNLLRPNFIVLTCNTAHYFFEELQAATDIPI
                     LHMPREAANELVRQHTTGRVAILGTEGSMKAGIYEREVKNLGFETVIPDTALQEKINY
                     LIYHEIKESDYLNQELYYEILEEAVERLNCEKVILGCTELSLMHEFAEDNHYPVIDAQ
                     SILADRTIERALAERSEALDTASEK"
     gene            complement(5661..6926)
                     /locus_tag="IWO89_000295"
     CDS             complement(5661..6926)
                     /locus_tag="IWO89_000295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-aspartate ligase"
                     /protein_id="HAQ0455083.1"
                     /translation="MMNSIENEEFIPILLGSDMNVYGMARSFNEAYGKICQAYASDQL
                     APTRYSKIVNVEVIPGFDKDPVFIETMLRLAKERYSDKSKKYLLIACGDGYAELISQH
                     KQELSEYFICPYIDYSLFERLINKVSFYEVCEEYDLPYPKTLIVREEMLVNGHLEQEL
                     PFEFPVALKPANSVEYLSVQFEGRKKAFILETREEFDLILGRIYEAGYKSEMIVQDFI
                     PGDDSNMRVLNAYVDEDHQVRMMCLGHPLLEDPTPASIGNYVVIMPDYNEKIYQTIKA
                     FLEKIEYTGFANFDMKYDPRDGEYKLFEINLRQGRSSFFVTLNGLNLARFVTEDRVFN
                     KPFVETTYGTNQSDKARLWMGVPKKIFLEYARENEDKKLAEQMIKENRYGTTVFYEKD
                     RSIKRWLLMKYMFHNYIPRFKKYFHVKEG"
     gene            complement(7189..10005)
                     /gene="uvrA"
                     /locus_tag="IWO89_000296"
     CDS             complement(7189..10005)
                     /gene="uvrA"
                     /locus_tag="IWO89_000296"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HAQ0455084.1"
                     /translation="MANDKITIHGARAHNLKNIDVTIPRDKFVVVTGLSGSGKSSLAF
                     DTLYAEGQRRYVESLSAYARQFLGQMDKPDVDSIDGLSPAISIDQKTTSKNPRSTVGT
                     VTEINDYLRLLFARVGHPICPNDHIEITSQSVDQMADQVMALPERTKIQVLAPIVVKK
                     KGQHKKIFERIQKEGYVRVRVDGEIYDLSEVPELEKNKKHDIAIVIDRIIVKEGIRSR
                     LFDSLEAALRLADGYALVDVIGEEEMLFSEHYACPYCGFTVGELEPRLFSFNAPFGAC
                     PDCDGLGVKLEVDQDLVIPDRTKTLSQGAIVPWNPISSQYYPQMLAQACESFGIDLDT
                     PFEELPEEHQEIILNGTDDLFHFHYENDFGGVRDVETTFEGVLKNIERRYHDTNSDFT
                     REQMRLYMTELTCQTCKGYRLNPQALSVQINGTNIGQTNELSIQKAVDFFSNLTLSEQ
                     EKVIAKPILKEVNDRLSFLENVGLDYLTLSRASGTLSGGEAQRIRLATQIGSNLSGVL
                     YILDEPSIGLHQRDNDRLIGSLKKMRDLGNTLVVVEHDEDTMWAADYLIDVGPGAGEQ
                     GGQIVAAGTPEEVANDENSLTGKYLSGKKSIPVPKERRKGNGKAIKITGASENNLKNI
                     SVEFPLGEFVAVTGVSGSGKSTLVNSILKKSLAQKLNKNSAKPGKFKTISGYESIEKI
                     IDIDQSPIGRTPRSNPATYTSVFDDIRGLFAQTNEAKMRGYKKGRFSFNVKGGRCEAC
                     RGDGIIKIEMHFLPDVYVPCEVCHGKRYNSETLEVHYKGKSIADILEMTVEDAVEFFK
                     HIPKIHRKLQTIVDVGLGYVTMGQPATTLSGGEAQRMKLASELHKISNGKNFYILDEP
                     TTGLHSDDIARLLHVLQRLVDAGNTVLVIEHNLDVIKTADYIIDLGPEGGEGGGTILA
                     TGTPEEIINVKESYTGHYLKKIMV"
     gene            complement(10015..12009)
                     /gene="uvrB"
                     /locus_tag="IWO89_000297"
     CDS             complement(10015..12009)
                     /gene="uvrB"
                     /locus_tag="IWO89_000297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrB"
                     /protein_id="HAQ0455085.1"
                     /translation="MIERDTSRHFELVSKYQPAGDQPEAINQLVDGVVGGKKAQILLG
                     ATGTGKTYTISNLIEKVNKPTLIIAHNKTLAGQLYGEFKEFFPNNAVEYFVSYYDYYQ
                     PEAYVPSSDTYIEKDSSVNDEIDKLRHSATSSLLERNDVIVIASVSCIFGLGSPFEYQ
                     KQVVSIRQGAELDRNQLIRDLVSIQFERNDIDFQRGRFRVRGDVVEIFPASRDERALR
                     VEFFGDEVERIREVNALTGEVLGETEHVAIFPATHFVTNDEHMEHAVANIKAELEQRL
                     TVLRNENKLLEAQRLEQRTNYDIEMMLEMGYTSGIENYSRHMDGRKEGEPPYTLLDFF
                     PEDFLIVADESHVTMPQIRGMYNGDRARKQMLVDYGFRLPSALDNRPLRLEEFEKHVN
                     QIIYVSATPGPYEHEQTDTVIQQIIRPTGLLDPVIEVRPIMGQIDDLVGEINERVEKD
                     QRVFVTTLTKKMAEDLTDYFKELGIKVKYLHSDIKTLERTEIIRDLRLGEFDVLVGIN
                     LLREGLDVPEVSLVAILDADKEGFLRSERSLVQTIGRAARNEEGKVIMYADKVTDSMR
                     LAMDETSRRRTIQQKYNEEHGIVPKTIIKEIRDLISITKESEDDTKEAVQVSYEEMTK
                     EEKDTLLMKLEKEMKDAAKALDFETAANVRDMILELKASK"
     gene            12268..13770
                     /locus_tag="IWO89_000298"
     CDS             12268..13770
                     /locus_tag="IWO89_000298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294446.1"
                     /note="The N-terminal region of this protein, as described
                     by TIGR01726, is a three transmembrane segment that
                     identifies a subfamily of ABC transporter permease
                     subunits, which specificities that include histidine,
                     arginine, glutamine, glutamate, L-cystine (sic), the
                     opines (in Agrobacterium) octopine and nopaline, etc;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HAQ0455086.1"
                     /translation="MKRKHLFHSFVFLMTGLVTFAFGHVAQADNTTTAESSTSETMQK
                     IEPKKDVYVIASDSAFAPFEFQNAKGEYEGIDVKLMENIAKLQGFNIRMDYRGFSAAL
                     QALESGQADGMIAGMTVTDERKKSYDFSDSYFDSGIQLAVKKGDTSIKSYEDLKGKTV
                     GAKVGTESAEFLSDHESEYGYSIKYFDAADQLYDGLKVGAIDAMMDDYPVIGYAIKQG
                     QELETPIKKESGGKYAFAVKKGQNPELLNMFNEGLKELKRTGEYDKIIKEYVADGSET
                     SSESSTADESTFLGLIQNNYQALLKGLWRTILLTLISFALAIVIGVIFGLFSVAPIRA
                     LRTIASIYVDIIRGIPMMVLAFFIFFGLPGIVGFTIPDFLAGVITLTLNASAYIAEIV
                     RGGINAVPVGQMEASRSLGLSYNRTMQKIILPQAIRIMIPSFVNQFVISLKDTTIISA
                     IGVVELLQTGKIIVARTTQSTYVYLIIAIMYLILITALTKLAKVLEKKVK"
     gene            13772..14509
                     /locus_tag="IWO89_000299"
     CDS             13772..14509
                     /locus_tag="IWO89_000299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010760672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HAQ0455087.1"
                     /translation="MAEKILVEHLVKKYGDNTVLNDINVSINEGDVVCIIGPSGSGKS
                     TFLRCLNRLEEPSDGEIIIDGAHLMDKSTDINLVRQHIGMVFQHFNLFPHLSILENII
                     LAPMDLKKESREEAEKKAIKLLQTVGLEDKKDMYPEMLSGGQKQRVAIARALAMNPDI
                     MLFDEPTSALDPEMVGDVLNVMKKLAEQGMTMVIVTHEMGFAKEVANRVMFIDDGNFL
                     EDGTPQQIFENPQNERTKDFLNKVLNI"
     gene            complement(14554..14727)
                     /locus_tag="IWO89_000300"
     CDS             complement(14554..14727)
                     /locus_tag="IWO89_000300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HAQ0455088.1"
                     /translation="MEKKNQAQLSEGGIDYVLDIINEPMVLSYAMEQVERLLVCAGKQ
                     AMRLIRLGEKIRR"
     gene            14703..15368
                     /locus_tag="IWO89_000301"
     CDS             14703..15368
                     /locus_tag="IWO89_000301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar diacid utilization regulator"
                     /protein_id="HAQ0455089.1"
                     /translation="MELDFSSPYYVVVGQLSDNQQLCQERINPLLELLERVFSYYGQA
                     LLTVHRQQIIILVNRISRASLLSLLDKIQTKFNKMYQLQLNFGIGSLCYTEQETPQSF
                     LHAKQVCEWIAFHQSVNEIRFFEDLDLGIVLPAIPSDQRTLYVKRILKSLTEEEVHLF
                     KKTLACFSKNNGSIKNCSEELFIHKNTLQYRLNKFHSLTGYTPRNYDDYHILKLAFLL
                     VQT"
     gene            complement(15450..16616)
                     /gene="dnaJ"
                     /locus_tag="IWO89_000302"
     CDS             complement(15450..16616)
                     /gene="dnaJ"
                     /locus_tag="IWO89_000302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010767443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HAQ0455090.1"
                     /translation="MATKRDYYEVLGLSKGASEDEIKKAYRKLSKKYHPDINKEPDAE
                     EKFKEVSEAYEILSDPQKRAAYDQYGHAGTDPNYGAGGAGGGFGGFGGFSGGGFGGFE
                     DIFDSFFGGGGRSVDPNAPRQGADLQYTVDLSFEEAIFGVEKEIKYNREEICHTCGGN
                     GAKPGTQPTTCHKCHGAGTINVERQTPLGRVMSRQTCDVCHGTGKEIKEPCPTCHGSG
                     HEKKAHSVKVNVPAGVEEGQQMRLAGQGEAGENGGPFGDLYVVFRVEESDIFDREGSE
                     IYYELPLSFVQAALGDEVQVPTVHGDVKLKIPAGTQTGTNFRLRGKGAPKLRGGATGD
                     QHVKVKLITPKNLNEQQREALRAFAEAGGIKVEEQQEDGFFDKFKDAFGGKKRK"
     gene            complement(16767..18596)
                     /gene="dnaK"
                     /locus_tag="IWO89_000303"
     CDS             complement(16767..18596)
                     /gene="dnaK"
                     /locus_tag="IWO89_000303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016635026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HAQ0455091.1"
                     /translation="MSKIIGIDLGTTNSAVAVLEGNEAKIIANPEGNRTTPSVVSFKN
                     GEIQVGEVAKRQAVTNPNTISSIKRHIGEAGYKVDVEGKSYTPQEISAMILQYIKGFA
                     EDYLGEKVEKAVITVPAYFNDAQRQATKDAGKIAGLEVERIVNEPTAAALAYGLDKTD
                     RDEKILVFDLGGGTFDVSILELGDGVFDVLSTAGDNHLGGDDFDNKIIDYMVAEFKKE
                     NSIDLSQDKMALQRLKDAAEKAKKDLSGVSSTQISLPFITAGESGPLHLEMTLTRAKF
                     DELTADLVERTKVPVRQALKDAGLSQSEIDEVILVGGSTRIPAVVEAVRKETGKEPNK
                     SVNPDEVVAMGAAIQGGVITGDVKDVVLLDVTPLSLGIETMGGVFTKLIDRNTTIPTS
                     KSQVFSTAADNQPAVDIHVLQGERPMAADNKTLGRFQLTDIPAAPRGIPQIEVTFDID
                     KNGIVNVSAKDLGTQKEQKITIKSSSGLTDEEIERMVKDAEANAEADKARKEEVDLRN
                     DVDALLFSVDKTLKELEGKVDEEEVKKAETARDELKAAVEANNIEEMKTKRDALNEIV
                     QNLTVKLYEQAAQQQAQENPEAAQGGADDVVDADFEEVDGDDK"
     gene            complement(18647..19210)
                     /gene="grpE"
                     /locus_tag="IWO89_000304"
     CDS             complement(18647..19210)
                     /gene="grpE"
                     /locus_tag="IWO89_000304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HAQ0455092.1"
                     /translation="MKENQEELDKEMTDAQPEPEIDVEAAEDSGISEAEAEEFETAKL
                     KAELEEMEDKYLRARAEIANMANRGKNEREQLQKYRSQDLAKKLLPSIDNLERALATE
                     VSDDQGAGLKKGVEMVLESLRNALEEEGIEKIPAKGEAFDPNLHQAVQTVPATEDTPA
                     DTIVEVLQEGYKLHDRVLRPTMVIVAQ"
     gene            complement(19304..20347)
                     /gene="hrcA"
                     /locus_tag="IWO89_000305"
     CDS             complement(19304..20347)
                     /gene="hrcA"
                     /locus_tag="IWO89_000305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heat-inducible transcriptional repressor HrcA"
                     /protein_id="HAQ0455093.1"
                     /translation="MLTKRQNDILRLIIQHYTSSGVPVGSKTLMSEGVKASSATIRND
                     MKALEDEGLLLKTHSSSGRIPSALGYRYYVDHLLKPARVAEDDLLLIRQSLGREFHEI
                     NEIIKQSAEILSELTSYTTFSLGPEIKDRRLTGFRIVPLNNRQIIAIVVTDMGNVESQ
                     VFTLPEYMGSQELEKMVQIVNDRLVGDPLMTVYHKLRTEIPMILHKYFQTTEGMSQLF
                     DTVLGHVFEDKVYVSGQMNLLDYEPRQDLDQFKSMFSFMKDSDGLTQMITPMDSNIHI
                     KIGAELGNDLLQNMSMIQASYEIDGHGRGTIALLGPTSMPYSRMFGLVDVYRRELAAK
                     LADYYRSLDLSNS"
     gene            complement(20581..21747)
                     /locus_tag="IWO89_000306"
     CDS             complement(20581..21747)
                     /locus_tag="IWO89_000306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-independent coproporphyrinogen III
                     oxidase"
                     /protein_id="HAQ0455094.1"
                     /translation="MIETINPAENKVSAYIHIPFCEHICYYCDFNKVFLEGQPVDEYV
                     EMLLNEMKIMLESHPTANLETLYVGGGTPTSLSAKQLDRLLSGAREILPFQNGNEFTV
                     EANPGDLTREKLQVMKNYGVNRLSMGVQTFDNRLLKKIGRKHTAEDVYQTMQFLEAEN
                     FTNVSIDLIYALPGQTLEGYLDTLEKALALDLPHYSLYSLILENKTMFMNWVRQGRLE
                     LPDQETETRMFEETIEAMEKHGRHQYEISNFGLSGHESQHNLMYWNNDHYFGFGAGAS
                     GYLGDIRYRNKGPIQHYLRPLRANERPVLEEEKLTRKNQIEEEMFLGLRKKTGITKKH
                     FYDRYQKTIESVYGDVLRELEQEGLLVNEEDRVYLTPRGTFLGNNVFERFLFEK"
     gene            complement(21763..21990)
                     /locus_tag="IWO89_000307"
     CDS             complement(21763..21990)
                     /locus_tag="IWO89_000307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosine deaminase"
                     /protein_id="HAQ0455095.1"
                     /translation="MKIAFHIFADFDLMPKVSFAITVYREAVKAPCSSDDNKQAFLEL
                     GFLDSKYAAYFNELVLERDFKKVENRHDFST"
     gene            22474..23064
                     /locus_tag="IWO89_000308"
     CDS             22474..23064
                     /locus_tag="IWO89_000308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine amidotransferase"
                     /protein_id="HAQ0455096.1"
                     /translation="MKTAITFLLEEYADWEGAYITSTLNQNEKWQNKTASIQSEVTSI
                     GGLKTKIDYLIEQIPEKVDLFIMIGGNSWDIKNHHLYQIIDKLLKTDVPVAAICGAVD
                     YLAQSGLLQGYKHTGNAQFPWLESENYQNKEDFIEQQAVKDRNLITANGTAPIEFTDL
                     ILQAVHFETEEIREKTIRLYKLGFYDYYKKYGDPFS"
     gene            23254..23967
                     /locus_tag="IWO89_000309"
     CDS             23254..23967
                     /locus_tag="IWO89_000309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0455097.1"
                     /translation="MKKLGFILLLVSLFLVGCTSPSKENSDPSATTDKSSIRQTSSAE
                     KSSTQKSASTETNTSSTSSKVSEPNTSSTSSSTVETNISSSTIDTTSSQSSQSIDPLA
                     GYSDLQIEYARVWLAIRGNTFRDTSGDFELHVIHYPAGTPIAPYDAGSAVYPKDVVML
                     TGKYSYQGVLVYSSNHDGTITSYPVPSHWQMPADQTSYPAFIRETTQEIIDNASVVAI
                     PTGNPNDVKQLIDVTVIDN"
     gene            complement(24082..25092)
                     /locus_tag="IWO89_000310"
     CDS             complement(24082..25092)
                     /locus_tag="IWO89_000310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LD-carboxypeptidase"
                     /protein_id="HAQ0455098.1"
                     /translation="MISYPLLERNATIGVTAPSSGVSEEHHSLLESALKRMEEKSFQV
                     VTGKTLWTQDKEKSGPARKRADEFNQMMQDENIQMIFPTWGGELLIEILDFVDFDALQ
                     PKWIVGYSDISTLLLAVTLKTGIATAHGTNLIDLRGEYLDETTAMWESVLKTREGESI
                     LQQSSEKYQKVWPHEHSSSSVFHLTEPTNWQSVSGDAVRMKGRLLGGCIDTIRHLIGT
                     PYGDVQSFKRKYNDGKPILWFLENCELSSVDLRRSLVQMKLAGWFDGSSGIMFGRSAA
                     EDLLDYKMKDVYEDLSKELKLPILYDIDCGHVPPQLTLINGAYAEVAVEDRKATVLQT
                     FI"
     gene            complement(25129..25611)
                     /locus_tag="IWO89_000311"
     CDS             complement(25129..25611)
                     /locus_tag="IWO89_000311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl-tRNA editing protein"
                     /protein_id="HAQ0455099.1"
                     /translation="MIDLAKKFLHENIYFELYHHRAIYTNEDALVIKKEQGFTGTETK
                     SLFLKDKKDNNYVYLTFTTKQTDFKALGKIVGKRLSIVSSERMEEQTGQKQGAVSPFG
                     YEKQIPIIIDEELLSNEKLVFAPGRPDQTMVIQTVDLNKIIQLLEMPIFLMPANEKNN
                     "
     gene            25761..26129
                     /locus_tag="IWO89_000312"
     CDS             25761..26129
                     /locus_tag="IWO89_000312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron chaperone"
                     /protein_id="HAQ0455100.1"
                     /translation="MEKFQPIINQIKEKEHQQKIKDLFAKITETYPQLDTTVKWNQPM
                     FTDHGTFIIAFSFAKNHFSIAPEKAAIRALEKNIQEAGYVYTDNVIKVPWKSVINWEL
                     IEQLISFNIEDKKGHDKFWR"
     gene            26594..27046
                     /locus_tag="IWO89_000313"
     CDS             26594..27046
                     /locus_tag="IWO89_000313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAQ0455101.1"
                     /translation="MKDFYSVNELAEQLGVTTRSIRNYLHEGKLKGTKVGGQWKFSER
                     NLFEFLYGDQAEEAAKEMQRFMLNAPITMRFNLQYRDFTAINQFREQLVQYHNDVYAN
                     KKDRLLQYDLYKDNHAEILIGGNFNYVVNFSQWINEKLLIQTDISLVS"
     gene            complement(27170..28021)
                     /locus_tag="IWO89_000314"
     CDS             complement(27170..28021)
                     /locus_tag="IWO89_000314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transporter"
                     /protein_id="HAQ0455102.1"
                     /translation="MGLLIALIPAVGWGIQPLILKKIGGRPTNEILGTGIGAVLVGLL
                     VQLFASPAGISVTTFLISLLSGAFWVIGQTGQYTSFNLIGVSKTMPISTALQLVGTSL
                     IGVFAFGEWSGSMGKIIGAAAIILLVFGSALTAISDDPGKKGGITKGISLLASTSIGY
                     WVYSALPKLVDASGLSIFFPQMIGVFLGAVIYVIFRKPTAFSEDKSWKATIVGAIFSV
                     SALAYIFSANENGVATAYIITQLNVVISTLGGMYILHENKTPKEKKLTIIGLLLIVVG
                     SMVTVFI"
     gene            complement(28034..28819)
                     /locus_tag="IWO89_000315"
     CDS             complement(28034..28819)
                     /locus_tag="IWO89_000315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HAQ0455103.1"
                     /translation="MYKELENKVAVVTGGSKGIGTAIAQRFGKEKMNVVVNYNSDKEG
                     AEKAVKAIEEAGGKAVSVQADVSSEEGIQRLLDTALENFGTLDVWVNNAGMENQHPTH
                     ELSLEDWERVLKVNLTGVFIGTKAALNYFTENNKKGNIINMSSVHEQIPWPTFAHYAA
                     SKGGVKLFTETVAMEYAPKGIRINSIGPGAIRTPINAAKFDDPEQKELLESMIPLGRV
                     GEPKEIAATAAWLASDESSYVTGITLFADGGMTLYPSFQGGKG"
     gene            complement(29171..30112)
                     /gene="ribF"
                     /locus_tag="IWO89_000316"
     CDS             complement(29171..30112)
                     /gene="ribF"
                     /locus_tag="IWO89_000316"
                     /EC_number="2.7.1.26"
                     /EC_number="2.7.7.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin biosynthesis protein RibF"
                     /protein_id="HAQ0455104.1"
                     /translation="MNIIKIRHPYQSEQIPEDDVVLVLGFFDGVHIGHQKVIKTGMEI
                     AQKEGLKLALMTFNQHPSIVFKKIDPSSVKYLTSLKQKEEKMAALGIDYLYEVDFTSS
                     FAHLAPQEFVDQYIVGLHAKYAVSGFDYTYGPKDIADVAHFPEYAKGRFKIVTVSKEE
                     EQGEKISSTRIRGLLDSGQVKEAAKLLGCTYEVEGVVVHGDARGRLLGFPTANVKVKS
                     TVHLPKEGVYVSEIKIGETWYPAMGSIGHNDTFGEGRELTVELYILDFNQDIYGEHVA
                     VRWNQFLRDQVKFSSVEELIEQLKEDEQATAEYFSQQ"
     gene            complement(30116..31039)
                     /gene="truB"
                     /locus_tag="IWO89_000317"
     CDS             complement(30116..31039)
                     /gene="truB"
                     /locus_tag="IWO89_000317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HAQ0455105.1"
                     /translation="MDGLLPLWKERGMTSHDCVFKLRKILHTKKIGHGGTLDPDVDGV
                     LPICIGKATKVIEYLTDSGKIYVGEITLGFSTTTEDRSGERVETKPVDMSLSEKKIDE
                     VMASFIGEITQIPPMYSAVKVNGKRLYEYARNNETVERPVRKAQIYRFIRTSELTRDE
                     VTGTISWRFEVECGKGTYVRTLAVDTGKKLGYPAHMSDLTRTASAGMSQKDALTLEEV
                     AQAVKNGTIDDCLLPIEKGVERFKRVDITDEVWQKVKNGMRLDYTVFALPEMPSEEIA
                     LFYKGKVVSIYQPNPKEKNKLKPSKVLRNEE"
     gene            complement(31407..33769)
                     /locus_tag="IWO89_000318"
                     /pseudo
     CDS             complement(31407..33769)
                     /locus_tag="IWO89_000318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625383.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoketolase family protein"
     gene            34217..34390
                     /locus_tag="IWO89_000319"
     CDS             34217..34390
                     /locus_tag="IWO89_000319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAQ0455106.1"
                     /translation="MIFLLTVNGPTNSNFYGAGSTDLLVTWLYLTVTKIDYNLASVIG
                     ILIFIYMQKFIYY"
     gene            complement(34547..34894)
                     /gene="rbfA"
                     /locus_tag="IWO89_000320"
     CDS             complement(34547..34894)
                     /gene="rbfA"
                     /locus_tag="IWO89_000320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HAQ0455107.1"
                     /translation="MANYRDRRVGQEILKEVNDILRKKVRDPRVENVTITDVKVTGDL
                     QQATIYYSILSDLASDKKTAQDGLNKASGLIRRELGHNLSIYKTPELFFELDESVVYG
                     NHIDELLRDLNKD"
     gene            complement(34921..37227)
                     /gene="infB"
                     /locus_tag="IWO89_000321"
     CDS             complement(34921..37227)
                     /gene="infB"
                     /locus_tag="IWO89_000321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HAQ0455108.1"
                     /translation="MGNKRIYELAKELKKSSKDVVEKAQQLGIDVKNHMGAITSNDEK
                     KLQQAFKNPSTKQQAAQQASRKPANQQPNDQRKKETTNNQQKNKNNRSYQDRGQGSGQ
                     VNQGKKPTTNQNNTGNTSSQNRQGTSQGGNQNRTNNNNKPNNNNRGKFNNNNRNRYNK
                     KGKKGKQQQSTKPAVPPRKFRELPDVLEYTEGMNVADIAKKIHREPAEIIKKLFMLGV
                     MVNQNQALDKDTIELLATDYGMEPQEKVQVDIADIDKFFEAEEVDPDKLVSRPPVVTI
                     MGHVDHGKTTLLDTLRHSRVTSGEAGGITQHIGAYQIDIDGKPITFLDTPGHAAFTSM
                     RARGASITDITILVVAADDGVMPQTVEAINHAKAAGVPIIVAVNKIDKPGANPQHVMQ
                     ELSEYELIPEAWGGETIFVEISAKFGQNIEELLEMILLVAEVEDLKADPTQRAIGTVI
                     EARLDKGKGPVSTLLVQEGTLHVGDPIVVGNTYGRVRVMTNDLGRRDKEAGPATPVEI
                     TGLNDVPQAGDRFVVFEDEKTARAAGEERAKRALLENRAATSRVTLDNLFESLKEGEL
                     KEVNVIIKADVQGSAEALAASLKKIDVEGVRVKIVHSAVGAINESDVTLAAASNAIII
                     GFNVRPTPQAKVQADTEEVDIRLHRIIYKAIEEIETAMKGMLDPEFEEKITGQMIVRE
                     TFKVSKVGTIAGAYVTEGYIRRDSGVRVIRDGIVIYEGQLASLKRFKDDVKEVKMGYE
                     CGAMIEKFNDIKVDDVIEGFIMEEVKND"
     gene            complement(37240..37551)
                     /locus_tag="IWO89_000322"
     CDS             complement(37240..37551)
                     /locus_tag="IWO89_000322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlxQ-related RNA-binding protein"
                     /protein_id="HAQ0455109.1"
                     /translation="MNTVNRQKAMNLIGLAMRAGKLITGEELTIAEIRKQKAKLVFVA
                     TDASENTRKKIKDKSSYYEVPCFELFSEAEITQMIGKPRKVFGIMDAGFAKKTKELIE
                     G"
     gene            complement(37548..37841)
                     /locus_tag="IWO89_000323"
     CDS             complement(37548..37841)
                     /locus_tag="IWO89_000323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlxR family protein"
                     /protein_id="HAQ0455110.1"
                     /translation="MKQRKIPLRKSVVSGEMKPKKEMVRITRSKEGEVSIDPTGKMPG
                     RGAYVSLEPEEVQQAWDKKILDRVLETTLTDEFYQELLDYVTHQKARKELFGK"
     gene            complement(37863..39038)
                     /gene="nusA"
                     /locus_tag="IWO89_000324"
     CDS             complement(37863..39038)
                     /gene="nusA"
                     /locus_tag="IWO89_000324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusA"
                     /protein_id="HAQ0455111.1"
                     /translation="MSKEMLNALDALEAEKGISKEIVIDALEAALVSAYKRHYGQAQN
                     VEVEFDQKKGKIHVYAVKEVTEEVMDSQLEVSLKDALLINPAYEIGDTIRFEVTPKDF
                     GRIAAQTAKQVILQRVREAERTIIYNEFSAYEKDIMQGIVERQDKRYIYVNLGKIEAV
                     LSKQDQMPNEFYQPHDRIKVYVSRVENTSKGPQVFVSRSHPDLLRRLFEQEVPEVYDG
                     IVEIVSIAREAGDRSKVAVRSNDPNIDAVGTCVGPKGQRVQAIVNELKGENMDIVEWN
                     EDPAVFIANALNPAQVMDVIFDETNPKACTVVVPDYQLSLAIGKRGQNARLAAKLTNH
                     KIDIKSESDMTEFYENQEQQKETEELHDEAIVQSDLTDDEYETIAFNNETVEEKPEA"
     gene            complement(39061..39534)
                     /gene="rimP"
                     /locus_tag="IWO89_000325"
     CDS             complement(39061..39534)
                     /gene="rimP"
                     /locus_tag="IWO89_000325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimP"
                     /protein_id="HAQ0455112.1"
                     /translation="MSSVVETVTEMVTPILDEQNFELVEVEFVKEGKSWFLRVFIDKE
                     GGIDIEECAFVSEKLSEKLDAADPDPIPQAYFLEVSSPGAERPLKKESDYEKALGDYV
                     HVSLYQPVDGEKQYEGFLQSFDAEQLTLKIRIKTREKELTFDRKNIAKARLAIQF"
     gene            complement(39679..44037)
                     /locus_tag="IWO89_000326"
     CDS             complement(39679..44037)
                     /locus_tag="IWO89_000326"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PolC-type DNA polymerase III"
                     /protein_id="HAQ0455113.1"
                     /translation="MSEKRDLFEKLLEQIQLEEAEKNHPLLTAGEMDSVVVHRKSRLW
                     EFTLHFSKILPIMLYRSLTQHLTIAFKDIAQVKLNILADDQTFDEQLLQDYWAQALEN
                     QQCDTPLAQQVLKTQVPVIKDRKVILPIDSTGAISYLKQQYLPLVEELFVSYGFPKFR
                     IEPEVDEQQAERVLKLFEERKQEQAEAFMKQAAESLVVHEQKKKERKEQSPALEGPIH
                     IQLGRNIPADEPTTPMISIVEEERRVTLEGYVFDKEVRELRSKRKILTLKITDYTSSF
                     IVKKFSNGEKDEQVFDAISVGSWLKVRGSIQEDTFVRDLVMNAQDIIEVKHTPRKDYA
                     PEGEKRVELHVHSNMSTMDATNSISDLVAQAGKWGHRAIAITDHGGAQAFPEAHSAGK
                     KAGVKILYGVEANVVDDGVPIAYNDAHEALSEATYVVFDVETTGLSAVYDTIIELAAV
                     KMYKGNVIESFDEFIDPGHPLSRTTVDLTGITDGMVRGSKSEEEVLRMFLEFSKDTIL
                     VAHNAAFDMGFLNTSYARYGIPEAANPVIDTLELARYLYPQFKRFGLGVLSKKFGVSL
                     EQHHRAIYDAEATGHLAWIFVKEAMDNHNMLYHDQLNEHIGEGDSYKRARPFHVTILA
                     KNQAGLKDLFKLISMSNVEYFERVPRIPRSQLKKMRENLLIGSACDKGEIFEAMMQKG
                     VEEARNRAKFYDYIEVMPKAVYAPLIEQELVKNEHDLEEIIQNLVEIGKSLDKIVVAT
                     GNVHYLNEEDAIYRKILINSMGGANPLNRHSLPDVHFRTTDEMLTAFHFLGEETAKEI
                     VVENTNKIADICEEVIPVKDELYTPKIPGSEDEISELSYTKAKQMYGDPLPEIIQKRL
                     KKELNSINGNGFSVIYLIAQKLVHKSNEDGYLVGSRGSVGSSFVATMTGITEVNPLAP
                     HYYCPECQYSEFFEDGTYGSGFDMPEKQCPKCGARLNKDGHDIPFETFLGFHGDKVPD
                     IDLNFSGDYQAEAHNYTKVLFGEDYVYRAGTIGTVADKTAYGYVKGYERDNNLQFRSA
                     EVDRLAKGATGVKRTTGQHPGGIIVIPDYMDVYDFTPIQYPADDQNSEWKTTHFDFHS
                     IHDNVLKLDILGHDDPTVIRMLQDLSGIDPQTIPTDDPEVMRIFAGPEVLGVSQEQIY
                     SKTGTLGIPEFGTRFVRGMLEETHPTTFAELLQISGLSHGTDVWLGNAEELIRRGDAT
                     LAEVIGCRDDIMVYLIHAGLDSGMAFKIMETVRKGQWNKIPDELRETYLSAMKENNVP
                     DWYIDSCSKIKYMFPKAHAAAYVLMALRVAYFKVYFPILYYCAYFSVRADDFDLVSMC
                     KGKDAVKQAMKEITDKGLDASVKEKNQLTVLELANEMLERGFKFGMIDLYKSDAVNFV
                     IEGDTLIAPFRAVPSLGTNVAKQIVEARKDGPFLSKEDLATRGKVSKTLIEYMNDNGV
                     LKDLPDENQLSLFDML"
     gene            complement(44249..45958)
                     /locus_tag="IWO89_000327"
     CDS             complement(44249..45958)
                     /locus_tag="IWO89_000327"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HAQ0455114.1"
                     /translation="MRQSKMLIPTLREVPNDAEVLSHQILLRAGYIRQVSAGIYSYLP
                     LANRVLEKLKRIMREEFEKIGAVEMLMPAILPAELWEESGRYETYGPNLYRFKDRNDR
                     KMILGPTHEETFTDLMRNEINSYKKLPLNLYQIQPKYRDEKRPRFGLLRGREFIMKDA
                     YSFHATEESLDETYKDYEKAYTEVFKRCGLEFRSIIGDGGAMGGKDSKEFMAISEIGE
                     DTICYSTESDYAANLEMATSYYVSKKSHETQLELEKVATPNVKSIDEVADFFSVEPQK
                     IIKSVLFIADEQPVLVLVRGDHEVNDVKLKNFLSADFLEEATEEEAQKYLGAEFGSVG
                     PVGVSDDVKVYADRYVQDLANAITGANETGFHYKNVNPERDFTVLSYEDLRFVQEGDP
                     SPDNNGVLAFTRGIEIGHIFKIGTRYSESMGATVLDENGREKFVIMGCYGIGVSRLLS
                     AIVEQHSDEQGINWPKGIAPFDLHIVQMNLKDEYQTNLTNELEEAMTKAGYEVLVDDR
                     NERAGVKFADSDLIGCPIRITVGKKAVDGIVEVKIKKTGEMVEVRKEELVNTLPILLN
                     QGE"
     gene            complement(46026..47294)
                     /gene="rseP"
                     /locus_tag="IWO89_000328"
     CDS             complement(46026..47294)
                     /gene="rseP"
                     /locus_tag="IWO89_000328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RIP metalloprotease RseP"
                     /protein_id="HAQ0455115.1"
                     /translation="MKTILTFIIVFGILVIVHEFGHFFFAKRSGILVREFAIGMGPKI
                     YGHQAKDGTTYTLRLLPIGGYVRMAGNGDDETEMAPGMPLSLLLNSDGIVEKINLSKK
                     IQLTNAIPMELSRYDLEDELTITGYVNGDETEVVTYPVDHDATIIENDGTEIRIAPKD
                     VQFQSAKLWQRMLTNFAGPMNNFILAILLFIILAFMQGGVQVTNTNRVGEIMPNGAAA
                     EAGLKENDEVVSVDGKEIHSWNDLTTVITKNPDKTLDFKIEREGQVQSVDVTPKSVES
                     NGEKVGQLGIKAPMNTGFMDKIIGGTRQAFSGSLEIFKALGSLFTGFSLDKLGGPVMM
                     YQLSSEAANQGVTTVISLMALLSMNLGIVNLLPIPALDGGKLVLNIFEGIRGKPLSQE
                     KEGILTLAGFGFLMLLMVLVTWNDIQRFFF"
     gene            complement(47455..48255)
                     /locus_tag="IWO89_000329"
     CDS             complement(47455..48255)
                     /locus_tag="IWO89_000329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HAQ0455116.1"
                     /translation="MKQRVVTAAVALVLFIPIIWIGGIAVELTAAILAVIGVYELFRM
                     KGLTLLSFEGVLSAVGAVLLVLPKERWFFFLPENVSTFMLFYLIVMILLGTSVISKNT
                     YTIDEAGFPVLVSLYVGIGFQNFVNARTESLAVLIFGLFIVWATDIGAYMVGRRYGQH
                     KLSPDISPNKTIEGALGGIGSAVVVAFLYLLLYPAKELFNHGMFVMLLMTIIFSAVGQ
                     FGDLVESAIKRHYDVKDSGNILPGHGGILDRFDSLLFVFPIMHLFGII"
     gene            complement(48252..49064)
                     /locus_tag="IWO89_000330"
     CDS             complement(48252..49064)
                     /locus_tag="IWO89_000330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenyl transferase"
                     /protein_id="HAQ0455117.1"
                     /translation="MLRFFPQKNKYIQEKSEFQFNTDGPIPQHIAIIMDGNGRWAQNR
                     RLPRVAGHKEGMETVKKVTKKASRLGVKVLTLYAFSTENWKRPKDEVSFLMQLPVDFF
                     DTFVPELIKENVKVHVMGYENVLPEHTQDAVRRAIEQTKNNTGMVLNFALNYGSRAEI
                     VTAVQEIAEEVAKGEIHAEEIDDELIAKHLMTGFLPKELQDPELMIRTSGEERISNFL
                     LWQIAYSELYFTKALWPDFDGAHLEEAIASYQNRDRRFGGVKKTEDEKGDQS"
     gene            complement(49259..49816)
                     /gene="frr"
                     /locus_tag="IWO89_000331"
     CDS             complement(49259..49816)
                     /gene="frr"
                     /locus_tag="IWO89_000331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HAQ0455118.1"
                     /translation="MADAIMTEAKEKMKKAAQNLQRELGQIRAGRANASLLDRITVNY
                     YGAPTPLNQMASIQIPEARVLMITPFDKSILQDVEKAIMASDIGISPTNDGNVIRLVI
                     PQLTEERRKELAKDVKKEAENAKIAIRNIRRDAIDEYKKQQKNGDITEDDLRGLEKDV
                     QTLTDNSIKEVDKIAADKEKELLEV"
     gene            complement(49819..50541)
                     /locus_tag="IWO89_000332"
     CDS             complement(49819..50541)
                     /locus_tag="IWO89_000332"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010761216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HAQ0455119.1"
                     /translation="MVTPKYQRVVLKLSGEALAGDAGFGIKPPVIKEIIQEIKEVHEL
                     GVEMAIVVGGGNIWRGQIGAQMGMERAQADYMGMLATVMNALALQDTLENVGVPTRVQ
                     TSIEMRQIAEPYIRRKAERHLEKGRIVIFAGGTGNPYFSTDTTAALRAAEIGADVILM
                     AKNNVDGVYSADPKVDANAVKFEELTHLEVIAKGLQVMDSTASSLSMDNDIPLLVFNL
                     NEHGNIRRAILGENIGTTVRGK"
     gene            complement(50677..51558)
                     /locus_tag="IWO89_000333"
     CDS             complement(50677..51558)
                     /locus_tag="IWO89_000333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Ts"
                     /protein_id="HAQ0455120.1"
                     /translation="MADVTAKMVKELREMTGVGMMDAKKALVEVEGDMEKAVDLLREK
                     GMAKAAKKNDRIAAEGLASVAVKGNTAAIVEVNSETDFVSKNEMFQDLVKEIAELVAE
                     NKPADMEAAMKIKTDKGTIESDLIEATQVIGEKISFRRFEVVEKDDNAAFGGYLHMGG
                     RIAVLTVLDGTTDETVARDVAMHVAAINPRYVNESQIPEAELEHEKTVLTEQALNEGK
                     PANIVEKMVEGRLKKFKAEIALVDQPFVKDPDMTVEKYVASKGATVKTFVRFEVGEGI
                     EKREDNFVEEVMSQVKK"
     gene            complement(51657..52439)
                     /gene="rpsB"
                     /locus_tag="IWO89_000334"
     CDS             complement(51657..52439)
                     /gene="rpsB"
                     /locus_tag="IWO89_000334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HAQ0455121.1"
                     /translation="MAVISMKQLLEAGVHFGHQTRRWNPKMKKYIFTERNGIYIIDLQ
                     KTVKLVDAAYDYMKSVAEEGGVALFVGTKKQAQEAIKEEATRAGQYFVNHRWLGGTLT
                     NWDTIQKRISRLKQINAMEEDGTFEVLPKKEVAGLNKERERLEKFLGGIADMPRIPDV
                     MYIVDPRKERIAVQEAHKLNIPIVAMVDTNCDPDEIDVVIPSNDDAIRAVKLITSKMA
                     DAFIEGNQGEDQVVEEDFTEENTATSIEEIVDVVEGDNSSAE"
     gene            52798..53277
                     /locus_tag="IWO89_000335"
     CDS             52798..53277
                     /locus_tag="IWO89_000335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArgR family transcriptional regulator"
                     /protein_id="HAQ0455122.1"
                     /translation="MRKAQRHLLIKQVIEEFPIRTQEELLNKLGHYGVTATQATISRD
                     IRDLKIVKAPDENGVSRFVLFREKGTPETRTEEEDRLVQMIEDIVLKVERVHFLTIVN
                     TLPDNAHLFASVLDEIKPPSIVSTIAGFDTIMVISENEEDAKRVEEFLHHPTEVSLF"
     gene            53494..54585
                     /gene="phnW"
                     /locus_tag="IWO89_000336"
     CDS             53494..54585
                     /gene="phnW"
                     /locus_tag="IWO89_000336"
                     /EC_number="2.6.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-aminoethylphosphonate--pyruvate transaminase"
                     /protein_id="HAQ0455123.1"
                     /translation="MYKLLTSGPLTTSETVKNAMLIDHCTWDQEYKEITQWIRKKLLD
                     LAQVSEGIYTTVLMQGSSSFGVESVLSSVIRPEDTVLICSNGAYRQRMINMCECLKLN
                     YTVYEVSEHQILQAHVITQLIEENPSISALAMVHSETTSGILNNLEEIAAVTQERDLL
                     FIVDAMSSFGGIPICVAELGIDFLISSSNKCIQGVPGFSFVLANKAELEKRKNVSRSL
                     SLDLYDQYRVMENENGKWRFTSPTHTVLAFQQALKELEAEGGIETRYSRYLANNHVLR
                     QKMTEMGFQPFIQEYQGPFITSFLYPKDWRFDFNHFYQYLKQNGFVIYPGKISDHECF
                     RIGNIGEIYPEDILELTAIIKDYKENVND"
     gene            54578..54706
                     /locus_tag="IWO89_000337"
     CDS             54578..54706
                     /locus_tag="IWO89_000337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonoacetaldehyde hydrolase"
                     /protein_id="HAQ0455124.1"
                     /translation="MIKTVIFDWAGTTVDFGCMAPVHAFRNAFLEKGIQLTDKEIR"
     gene            54710..55255
                     /locus_tag="IWO89_000338"
     CDS             54710..55255
                     /locus_tag="IWO89_000338"
                     /EC_number="3.11.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonoacetaldehyde hydrolase"
                     /protein_id="HAQ0455125.1"
                     /translation="MGKLKWDHIQQLLSLPSVKKQWIAIYGQLPQEKEVEELYQQFEH
                     YLFKDLVKHSTLKPDTLETIERLRNAGINIGSTNGFNAPMMTIVAETAKKERYSPDFM
                     ATFEDVEGYGRPFPYMIHKNMQYFKNKSVDEVIKVGDTVSDIQEGKNAGVLTVGVIEG
                     SSLMGISYDEYQKLEMGKKIV"
     gene            complement(55709..57400)
                     /locus_tag="IWO89_000339"
     CDS             complement(55709..57400)
                     /locus_tag="IWO89_000339"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HAQ0455126.1"
                     /translation="MNNKDLVAKAVYDAVKDDLTLEQVEQLLENPKSAEHGDVAFPAF
                     SLAKVYRKAPQQIAADLAEKIDSANFEKIEVVGPYLNFFMNKELISKKVLQTVVKEKE
                     HYGDSNIGNQGTVPIDMSSPNIAKPISMGHLRSTVIGNSIGFIMEKIGYQPIRINHLG
                     DWGTQFGKLIVAYKKWGTEEAVKAEPINELLRLYVQFHEVAETEPELNEEARAWFKRL
                     EEGDKEAIQLWQWFRDESMKEFNKIYDLLEVRFDSLNGEAFYNDKMDEIVKLLEEKHL
                     LNEDKGAEIVDLSAYDLNPALIKKSDGATLYITRDLAAALYRKRTYDFKQSLYVVGNE
                     QSYHFKQLKAVLKEMGFDWSDDMHHIPFGLITQGGKKLSTRKGKIVLLEEVLNEAIQS
                     AKEQISEKNPDLENKDAVAKQVGVGAVIFHDLKNDRLNTFDFNLEEVVRFEGETGPYV
                     QYTHARAVSLLEKAGFVPSETADYALNDDTSWEVVKLVQKYPETVLSAGEKYEPSVIA
                     KHAIKLAQAFNKYYAHTKILADDEQKEARLALVYAVTVLLKEDLRLLGLHAPDKM"
     gene            complement(57820..58767)
                     /gene="arcC"
                     /locus_tag="IWO89_000340"
     CDS             complement(57820..58767)
                     /gene="arcC"
                     /locus_tag="IWO89_000340"
                     /EC_number="2.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamate kinase"
                     /protein_id="HAQ0455127.1"
                     /translation="MAKRKVVVALGGNAILSTDASAKAQQEALMETAKYLVKFIEQGD
                     ELIISHGNGPQVGNLLIQQQAADSEKTPAMPLDTCVAMTEGSIGYWLQNAMGEVLKEK
                     GIDKDVVSLVTQVIVDENDPSFKNPSKPVGPFYTEEEAKEQMNADSTVTFKEDAGRGW
                     RKVVASPKPISIKEARVIETLVDQGVITVSVGGGGIPVVETATGLEGREAVIDKDFAS
                     EKLAEIIDADLLIVLTGVDNVYVNYQKPDQKKLETVTVSEMKQYIDEKQFAPGSMLPK
                     VEAAIAFVEAKPNAKAIITSLENIENLLASEEGTIIVAD"
     gene            complement(58882..59901)
                     /gene="argF"
                     /locus_tag="IWO89_000341"
     CDS             complement(58882..59901)
                     /gene="argF"
                     /locus_tag="IWO89_000341"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003073325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HAQ0455128.1"
                     /translation="MKESVFQGRSLLAEKDFTKEELQYLIDFSEHLKDLKKRGIPHHY
                     LEGKNIALLFEKTSTRTRSAFTTAAIDLGAHPEYLGANDIQLGKKESTEDTAKVLGRM
                     FDGIEFRGFSQKMVEELAEFSGVPVWNGLTDEWHPTQMIADFLTIQENFGTVEGITVA
                     YCGDGRNNMANSLLVTGAILGANMRIVAPKELQPEEEIVKMAEGFAEKSGAQLMITDD
                     VDKGVDGADVLYSDVWVSMGEEDKFEERIKLLKPYQINMEMVEKTHNTDRLIFLHCLP
                     AFHDTNTVYGEQMKERFGITEMEVTDEVFRSKYARQFDQAENRMHSIKAIMAATLGNL
                     FIPRV"
     gene            complement(59992..61221)
                     /gene="arcA"
                     /locus_tag="IWO89_000342"
     CDS             complement(59992..61221)
                     /gene="arcA"
                     /locus_tag="IWO89_000342"
                     /EC_number="3.5.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine deiminase"
                     /protein_id="HAQ0455129.1"
                     /translation="MDKPIHVFSEIGKLKTVLLKRPGQEVENLTPDIMDRLLFDDIPY
                     LPIAQEEHDNFAKTLQNEGVETLYLEKLAAEAIDAGNVKEQFLNKMLDESHIASNAVR
                     EGLHEFLLSMETQEMVDKIMAGVRTKDIEVRSSSLYDLSADDDYPFYMDPMPNLYFTR
                     DPSASMGNGMTVNKMTFEARRRESMFTEYILKHHPRFANKGIEVWLDRENPDHIEGGD
                     ELILSDKVVAVGISQRTNAKALEKLARHLFAKNSGFEKVLAIKIPNNRAMMHLDTVFT
                     MVDHDKFTIHPAIQSKDGKMDVFTIEPDGDDIKITHSDNLHETMKAALGLDDLVLIPT
                     GNGDEIVAPREQWNDGSNTLAIAPGVVVTYNRNYVSNELLRSYGIKVLEINSSELSRG
                     RGGPRCMSQPLVREDLK"
     gene            61682..62383
                     /locus_tag="IWO89_000343"
     CDS             61682..62383
                     /locus_tag="IWO89_000343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HAQ0455130.1"
                     /translation="MRVNKIDFHFSKLRYQPDFEHFSDEEFQQIKEHTVLRSYKKGQI
                     LFDDGDPRQRLYILTKGLVRLERYDESATYYYYDYVNTNNLFPMSGMFGEETYTYTAQ
                     AMTDIETFYLPVSYYEKLARKNPEQLIYMIQKISKVVEIHELRIQIGLTSSAFDRVKQ
                     NLFILKEELGVPTKDGHITIPYPITLKELAVNSGTTRETAGQVIKQLRDSGLLDYDKK
                     RFTFCKTATTPAMRE"
     gene            complement(62992..64008)
                     /gene="tsaD"
                     /locus_tag="IWO89_000344"
     CDS             complement(62992..64008)
                     /gene="tsaD"
                     /locus_tag="IWO89_000344"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HAQ0455131.1"
                     /translation="MNEELILAIESSCDETSVAVVRNGTEILSNIVASQINSHKRFGG
                     VVPEVASRHHVEQITLCLEDALVEAGVSAEDLSAVAVTYGPGLVGSLLIGISAAKAFA
                     WAHQLPLIPVNHMAGHIYAARLVKPFQFPLMALLVSGGHTELVYMQEDGSYEIIGETR
                     DDAAGEAYDKVGRVLGLSYPSGKEIDQLAHQGKDNYHFPRAMIHEDNYDFSFSGLKSA
                     FINLVHNAQQRGEDLDKNDLAASFQASVIDVLITKTLRACQNYPVKQLIIAGGVAANQ
                     GLREGLQDALAAKLPEVELVIPPLRLCGDNAAMIGAAAHVEMQKRNFASYQLNADPSL
                     VLSE"
     gene            complement(64005..64469)
                     /gene="rimI"
                     /locus_tag="IWO89_000345"
     CDS             complement(64005..64469)
                     /gene="rimI"
                     /locus_tag="IWO89_000345"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HAQ0455132.1"
                     /translation="MICRKKDFDEAYLAECVWMISENAYPHGSPWTKMQFLADIQQPH
                     SDYLLLAEDNQICGFIGYSKVIDEVEITNIAVAVSEQGKGHARHLLQLLITEQIKDSL
                     HVFLEVRLSNEAARKLYESEKFRILGKRKSYYRNPTEDAIIMSRKLKTETMK"
     gene            complement(64476..65018)
                     /gene="rimI"
                     /locus_tag="IWO89_000346"
     CDS             complement(64476..65018)
                     /gene="rimI"
                     /locus_tag="IWO89_000346"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HAQ0455133.1"
                     /translation="MLKKFRAYARSFFQDKMIYTEKTVEQIDRRYVLRELTTEDIKEL
                     LDVEREVYNGELPWTKSAFISELKSPLPHLYLGIFDNGRMIGFIGSRAFGLDCHITNI
                     AVRPAYQRKRIGSLLIDEIENFAIMNRCETMSLEVRMSNQDAQRLYRKLGFQATTIKK
                     RYYTETKEDALDMIKLLENE"
     gene            complement(65002..65826)
                     /gene="tsaB"
                     /locus_tag="IWO89_000347"
     CDS             complement(65002..65826)
                     /gene="tsaB"
                     /locus_tag="IWO89_000347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HAQ0455134.1"
                     /translation="MYYCTSFAFGIFILRIKKYATMEGENVIAERIKYMITLGIDTAN
                     QTLAIGVVEDEQILGQIQTNIKRNHSVTLMPAIDQLFADLQISPKDIDRIAVSDGPGS
                     YTGLRIGVTTAKTIAYTLDKELVGVSSLKTIAANCIGVDNIIVPMFDARRKNVYAGAY
                     RWKENRLETVLEDVHISASDLFTQLQKLNEAIYFVGSDCHKFFSEIKEILPSAMINTV
                     PAWDIPSGVTVAQLGALVEPVAEKQAFLPRYLKRVEAEEKWLETHTPGDESYVEKI"
     gene            complement(65915..66895)
                     /locus_tag="IWO89_000348"
     CDS             complement(65915..66895)
                     /locus_tag="IWO89_000348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HAQ0455135.1"
                     /translation="MATIKDIAQLAGVSPATVSRVLNYDPDLSVGLATKQKIFEAAER
                     LNYTKHRKNAANTKQTLRLIQWYDDTEELEDLYYLSIRLGIEKKAEEANVTLLKETWD
                     SISDLPADGTIALGKFDEAQLLEMKGSQNALLLVDSDGTSQGIDSLVVDFKSSVQKVI
                     EHFVSHKAQHIAMLAGTEYTKKNHQRLKDPRIMAFQQVLAEKGYDEGPIIEADFSVES
                     GYQAVKQYLKTNPHVPDALFAANDALAIGALKAIQEKGLVVPEDISVMGFNDVSVAKY
                     VTPSLSTVKVYTEWMGELAVETILQLSNSKAPVSRKIIVETELIIRESTK"
     gene            complement(66919..68403)
                     /gene="galT"
                     /locus_tag="IWO89_000349"
     CDS             complement(66919..68403)
                     /gene="galT"
                     /locus_tag="IWO89_000349"
                     /EC_number="2.7.7.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose--hexose-1-phosphate
                     uridylyltransferase"
                     /protein_id="HAQ0455136.1"
                     /translation="MSISQTICDFATLAIEAGGWMEMDRLYLQNRLLGMVGEDALESI
                     EVRPVKEESVDLLDQLVAKARENGVLSELPAEQEIFEAQLMDFLTPPPSVVNAFFAQH
                     YSKSPEEATEYFFNLSKKNDYIKTRAIAKNIKFPAESEYGELEITINLSKPEKDPKQI
                     AAEREAVKVDYPTCMLCMENEGYRGRLNYPARTNHRIIRMNLDGESWGFQYSPYAYYN
                     EHSIILSEQHRPMKITKETFARLLRITEVLPHYFVGSNADLPIVGGSILSHDHYQAGR
                     HEFPMAKAPIEKLIKLEDYPQVIAGVVKWPMSVIRLQSTEKNLLIEAANSVLEKWKAY
                     SDETVSIRAFSADGTPHHTITPIARRRDNYFELDLVLRDNNVSEEHPDGIFHPHKDVQ
                     HIKKENIGLIEVMGLAVLPPRLKPELVEVEHFLLGEENQMAEYHRPWAEQLKKEHPDL
                     TKDTVTDVVQKSVGQVFARVLEDAGVFKRDEKGQAAFERFTAIL"
     gene            complement(68415..69404)
                     /gene="galE"
                     /locus_tag="IWO89_000350"
     CDS             complement(68415..69404)
                     /gene="galE"
                     /locus_tag="IWO89_000350"
                     /EC_number="5.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010767298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
                     /protein_id="HAQ0455137.1"
                     /translation="MTILVLGGAGYIGSHAVDQLVQKGYQVAVVDNLLTGHKQAVHPD
                     AHFYEGDIRDKEFLRSVFEKEPIEGVIHFAASSLVGESVEKPLMYFNNNVYGMQILLE
                     VMHEFNVNKIVFSSTAATYGEPKESPITEDTPANPKNPYGESKLMMEKMMKWCDQAYG
                     MRYVALRYFNVAGAKADASIGEDHTPETHLVPIILQVALGQRKALAVYGDDYDTPDGT
                     CIRDYVQVEDLIAAHILALEYLKEGNESNFFNLGSSKGYSVKEMLEAAREVTGKEIPA
                     EIAPRRAGDPSRLVASSEKAREILGWKPEYTDIKAIIKTAWDWHVSHPNGYEE"
     gene            69652..69819
                     /locus_tag="IWO89_000351"
     CDS             69652..69819
                     /locus_tag="IWO89_000351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3042 family protein"
                     /protein_id="HAQ0455138.1"
                     /translation="MKKFVSGILVGSLATAAAVAGLVASVKKTVIDPIDEKEAMIEEN
                     RKKAMRKRVSR"
     gene            complement(69881..71692)
                     /locus_tag="IWO89_000352"
     CDS             complement(69881..71692)
                     /locus_tag="IWO89_000352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD/NAD(P)-binding protein"
                     /protein_id="HAQ0455139.1"
                     /translation="MKIGIIGAGPRGLSVAERILHNNQDRHSLELFLFDPNGPGGRVW
                     RLNQPPELLMNSVSQQVTLFTDETLSSGGLVTQGPNLFQWSKGPATDYIEQTVTKNKQ
                     LIIEEINRLEKDQQSTRCLYGLYQRWFFSKLQEEFPASIQFVYSLVKKVSKRKDGYLL
                     QTDQQSYSIDQLVLASGHWENDETEEERVWVDYAKKNVLLYQLPSNPADVDLSKIPPK
                     ENVFLRGLGLSFFDYVGLFTVSRGGRFENEKGKLVYHPSGKEPIVYSGSRRGLPYYPR
                     GRNQKQGGAMAWPRLLTKENLEQWHQSGFLTGETFFDYLKKDVELFYYKKIIEEYHLP
                     IPKKNFEHDFLSLSSEECLGKHPALIPYRWDWSYLETPLAYQNEPFSEASRIFIEGQI
                     KEAEKGNCTGAFASTFDALKDWRDPIRQAIEWHVFSAKEYKEILWGWFTHLNSFLTIG
                     PPIIRTKELAALIDAGIFHLIEPPFDIQQKHNRYVISLQDKEISSRYLIEARLPQTSF
                     SRSSNPVIKNLKENKLVRPFSFSDNTGSYHSEAIDVERGTNRVIDASGEIQSDFYCYG
                     IPVEGVDWLTAAVARPYTDPWNLRQADKIAQLILTDK"
     gene            complement(71689..72054)
                     /locus_tag="IWO89_000353"
     CDS             complement(71689..72054)
                     /locus_tag="IWO89_000353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF488 family protein"
                     /protein_id="HAQ0455140.1"
                     /translation="MIKLKRVYLDAEKEDGYRILVDRFWPRGISKEKAQLDLWLKEIA
                     PSNEARKTFDHVPERFASFAKKYQQELIFEEEKKQAIFKLSQLMKEHPVITFVYGAKN
                     EHQNHAVLLKKFIEMEACK"
     gene            complement(72217..72612)
                     /locus_tag="IWO89_000354"
     CDS             complement(72217..72612)
                     /locus_tag="IWO89_000354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HAQ0455141.1"
                     /translation="MVLWVINIILLLIVLAIILWEVYLRVMAKRSATTLTEEEFREGM
                     RKAQVIDVREKDVFDAGHILGARNIPYTVLKDSLGSIRKDQPVYIYDQKKSLSIRTAN
                     KLRKAGYQEIYLLKGGYDGWSGKIKSKKV"
     gene            complement(72630..73592)
                     /locus_tag="IWO89_000355"
     CDS             complement(72630..73592)
                     /locus_tag="IWO89_000355"
                     /EC_number="2.7.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family glucokinase"
                     /protein_id="HAQ0455142.1"
                     /translation="MDKKIIGIDLGGTTAKFAILTPEGEIQQKWSIDTNILDDGKHIV
                     PEIIESINHRLNLYNMKAEDFIGIGMGTPGSVDSEAGTVIGAYNLNWTEVQFVKKLIE
                     AGTGIKFAIDNDANVAALGERWKGAGENDPDVVFVTLGTGVGGGIVAGGNLIHGVSGA
                     GGEIGHITVDPEGFECTCGKRGCLETVSSATGVVRLGRFLAEEYAGDSKLKAMLDNGE
                     EVTSKDIFEMAQEDDPFALMVVDRVCFYLGLACGNLGNTLNPSSIVLGGGVSAAGEFL
                     RSRVEKYFKEFTFPQVRESTKIKLAELGNEAGVIGAASLALKFA"
     gene            complement(73592..73804)
                     /locus_tag="IWO89_000356"
     CDS             complement(73592..73804)
                     /locus_tag="IWO89_000356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqgQ family protein"
                     /protein_id="HAQ0455143.1"
                     /translation="MESLYDVQQLFKKFGIFIYVGARIYDIELMTIELRNLYDSRLID
                     HDTYMTAWRILKREHRIEESRKKGNL"
     gene            complement(73825..74523)
                     /locus_tag="IWO89_000357"
     CDS             complement(73825..74523)
                     /locus_tag="IWO89_000357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HAQ0455144.1"
                     /translation="MNYQQQIKLRAWLRRPLWTYAFLGIQTAVFLMMELFPRLEIPYY
                     AGMYGPYLVHFNEWWRLVTPIFIHFGLMHFVMNSLILYFMGQQIEAIYGHWRFFLIYM
                     FSGIMGNTASFAFNEANVLSGGASTSIFGLFGALFILGFHFKHNPAIQQLVRHFLLFI
                     VLTFVFGLLDTSVDVWGHVGGLIGGLVLGNVLGLPKQQTSYSIHQRILSTLVFVFLFV
                     ICILLGLKKYGLLV"
     gene            complement(74543..75085)
                     /locus_tag="IWO89_000358"
     CDS             complement(74543..75085)
                     /locus_tag="IWO89_000358"
                     /EC_number="6.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-formyltetrahydrofolate cyclo-ligase"
                     /protein_id="HAQ0455145.1"
                     /translation="MDQLKVRLRRKGIEKLKNLAKSGKKKEKEQRIYKQLFASEYWKS
                     AKVIGTTLSNEFELDTQPVIQRAEKEQKKIVVPKTLPKRQMAFYEMDEQSVLVRSSFG
                     VLEPQSEKLYLPEMIDLLIVPGIIYNRAGYRIGFGGGYYDRYLNNFHKKTCSLVFREQ
                     LNEQWQPESFDQKIQQLFID"
     gene            complement(75217..76221)
                     /locus_tag="IWO89_000359"
     CDS             complement(75217..76221)
                     /locus_tag="IWO89_000359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HAQ0455146.1"
                     /translation="MKKIYWTFYFLLFVLVILSIGSLMIGTPFIYLDQLFATFIGKES
                     SSLALIVREFRVPRLLISLLAGACLGVSGYILQGVTRNELADSSILGINAGAGFLVML
                     YLGFFSHYSLPFLLLVVAFLGGVLAACCVYLAAYDRNGFLGMNRILLSGIAVNAGLSA
                     LTLLCTIQLSKENYGFVNAWLAGSIWGASWPYVWALLPWAVILIPLGGLYAQRIVLLS
                     FGQERAQGLGLNVAKSQKILLLLAVALASGSVAVTGSLSFVGLLAPHAAKLVVRKENN
                     WTFVLSGLFGSIFLLSADMVARVILPSGEVPTGILIALFGAPYFLYALFIRQKHALNS
                     "
     gene            complement(76218..77207)
                     /locus_tag="IWO89_000360"
     CDS             complement(76218..77207)
                     /locus_tag="IWO89_000360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HAQ0455147.1"
                     /translation="MRTMKKNRLLLLVCFVLLIIFFVALSNGAATVEKADFWQALFDF
                     DRSKQSHQIIRNIRLPRVAGAFLVGSCFALSGALMQGVTRNPLADSGLLGINAGASLA
                     MALSFAFWPKASDFSVFLMSLAGSFAVTLLVFGFLQFSKMGMNPLQLILAGVAISSFF
                     TAISQALTQFFNLQQDLAFWFVGGAANITWKQLAVLFPLYILAVLVSFSLGKAISLIH
                     LSETHALALGSHPKRTRLIVFCLVAVLAAIAVSLVGPVSFIGLLVPPVVRGMVGTDYR
                     YVLPVSFLVGGGLVMLADFTARMINPPFETPFGLVIALIGVPFLLIQVRREQR"
     gene            complement(77204..78010)
                     /locus_tag="IWO89_000361"
     CDS             complement(77204..78010)
                     /locus_tag="IWO89_000361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ0455148.1"
                     /translation="MTRLRAADIAAGYENKKIIENMSVTIPEGKITSLIGPNGSGKST
                     LLKSFTRILPVKSGKIIVNNSDISTYSSKELAKKIALLAQTSEHPTGMTVEEVVSYGR
                     YPYQKLFSGMDTQDHEAIEWALDATGLAGFRMRDIATLSGGQTQRVWIAMALAQETDI
                     LILDEPTTFLDPAHQLEILQLLTEINQMKQTTILMSIHDINHASRFSDYLLAMKNGKL
                     ITEGTPEEVITKECMKKIFEIDATIVTLPRTNKPVFLTYDLLPKEQEDQL"
     gene            78176..79132
                     /locus_tag="IWO89_000362"
     CDS             78176..79132
                     /locus_tag="IWO89_000362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HAQ0455149.1"
                     /translation="MKLKTKVFTLSSILLLAGVLSACSPSSDKETATTANSTTSSNST
                     HTVTDTLGHKVEIPNAPKRIIGSYLEDYLIALGEKPIAQWTVGSGSIQDYLQDDLKDV
                     PTISYDLPYEKVLSFEPDLLLISSSATVEGGKYEQYSKIAPTYVVKNGTDVTWEEQLK
                     DVGKALAKEKEAEKIISDYQKSIKKTREELADKIENKTAAVLWVTNNSAFMVSKNRSS
                     GRIVYDDLEFGVPDLVEEVSKKATSDWSAVSSEKLAELDADYIILVNSDKDAAMFNEP
                     SWKNLKAVTDNHVLQFGPESSWLYNGPIASQEMVNDIKEKLN"
     gene            complement(79209..79727)
                     /locus_tag="IWO89_000363"
     CDS             complement(79209..79727)
                     /locus_tag="IWO89_000363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HAQ0455150.1"
                     /translation="MFISMWAQDKNGLIGKNGLLPWRLPNDMRFFREHTMDRILVMGR
                     KTYEGMGDLSLPYRHIIVLTTQPNFRTKENAEVMHSIDELLSYSQTISEDIYVSGGSR
                     IFQELLPYTGRIWRTLIDSTFEGDTYIGNIDFSDFALIEEYEGITDQENLYAHRFQKW
                     ERVKNRVASKEE"
     gene            complement(79815..79964)
                     /gene="rpmG"
                     /locus_tag="IWO89_000364"
     CDS             complement(79815..79964)
                     /gene="rpmG"
                     /locus_tag="IWO89_000364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016172256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HAQ0455151.1"
                     /translation="MRVNITLECTSCKERNYLTSKNKRNNPDRLEKQKYCPRERKVTL
                     HRETK"
     gene            80192..80638
                     /locus_tag="IWO89_000365"
     CDS             80192..80638
                     /locus_tag="IWO89_000365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2188 domain-containing protein"
                     /protein_id="HAQ0455152.1"
                     /translation="MPWNMKDFPASMKNLDKLTRKKAIDIANALLDEGYPDNRAIPIA
                     IDQAKKWYEDASESEKRTFEKEKNPSKTDEHDTNPRAGKLLDSDVIVEYEEEQWIVKS
                     KGAKKASNHFDTKKEAIEKGKQVAQNKESTLFIYKKDGTKEKEIDY"
     gene            complement(80833..82728)
                     /gene="asnB"
                     /locus_tag="IWO89_000366"
     CDS             complement(80833..82728)
                     /gene="asnB"
                     /locus_tag="IWO89_000366"
                     /EC_number="6.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase (glutamine-hydrolyzing)"
                     /protein_id="HAQ0455153.1"
                     /translation="MCGIVGFVNDKDNKKTIINTMMDRIVHRGPNSSGEYIDKHVALG
                     FRRLSIIDLEGGTQPIYNEDRTKIIIFNGEIYNYQPLREELIAAGHVFQTHADTEVLL
                     HGYEEWGTELLQKIRGMFAFAIWDNEKNELFGARDHFGIKPYYYAEMNGTFMFGSEIK
                     SFLPHPDFNKELNKEALKPYMTFQYSPLNGETFFKDVYRLPEGHYYTYKDGKIDIQQY
                     WDADFETKETHSRQEWIEKIDETVQASIEAHTVSDVEVGSFLSSGVDSSYVTSVLKPD
                     HSFSIGFDDKTYNEAIEARKLTELLDLDNTAAVIDGDMSFKAFPLIQYHLDEPDSNPS
                     CVPLYFLANLASQSVRVVQSGEGADELFAGYQTYGFHTNSKFIRVIAQGLKKLPKGTR
                     YNLGRKIGKMKNFHGRIHLYESLAPAKEYFIGHARVFEESEASEVLTPKYQTAPSVDE
                     IMTVHYEKTEGIKDEVNKMQYVDLHQWMPKDILLKADKLSMASSLEVRVPLLDIEVMK
                     LAQQIPSKFLLNQNNTKDIFRQAANKHLPEEWSNRVKLGFPVPIKAWLKEEHGYEQVK
                     ALFEADFAKEFFDQEKIMKLLDDHHEGRKEEQRKIWTIFSFLTWYKVYFVDETIPQAE
                     AIDYVTV"
BASE COUNT        23581 a        17673 c        14335 g        27412 t
ORIGIN      
        1 tctataaaca tcaccccaaa taaaacagaa aacagtataa gcattataaa aattatcaac
       61 aaccttgatc cttcttttat agattcagga aacagtataa ttttagtact agataaattg
      121 tttacaccga ttagtgttaa aatatcttta ttcttcttgc taacataacc ctttatgcac
      181 aaaagaacag ctatacttaa agtaacaaat aaataacgta ttccaataga aaaatcacta
      241 taaatactct ctagcatagg accgacaatt gaagaagtca ctactgcaaa tgctggacct
      301 atccaagcaa cattagaatg ttctttctct cttttccttt gtaaatcttt gttgagtatt
      361 aatctattta tttcttctgc aggcaagtta attttcataa ccttatgggg gattatccaa
      421 ttaaaaatcg gaaaaaggta gcctccccaa aaagtatagt gatctgtgtc aatcagataa
      481 taacctgatt ttgattccag aataacatat ctcaaagccg aacgaaaatt tttcatgaat
      541 atccataatc tcactgtttc cacacctaat tcctccaatt ccaattaaat ggatttatag
      601 catcaccaat tatgttaact gcctgaccaa cctcattgat aaaattggca ccactatccc
      661 atgcttcaac aatgctatat atctatcaaa caaaaaatgg aaaactaaat actcgcgcac
      721 ataaatttta cattctgttt tttataagac taataataat cattgaaaaa atttcttatc
      781 ggtggcgcat attcccaatt gattaatgtg atcccatatt ctgcaagtat tttacgagct
      841 tcttctaagt catagtgaga aaaatgcata aaacctgcaa tacgatcacg gactgtgagc
      901 tgtggcttgc ttccaccatc attcataaaa tatccagcag agccactact taaagtgatt
      961 gttacatctt cactttctat tattaagaat tcataatgaa atgctttcaa ctctccacta
     1021 ttatttaaaa gttttatact ccaacttcgt ctaggttgag ttgtcacatt ccctctaaaa
     1081 cttcctataa attctgcaat caccgtatct tcaatcaaaa ttctttcctc atctatttca
     1141 tattcaagac taaacggaaa ttctccatat gtaatctctg gcaatggtgg tgctgtagtt
     1201 ctccaaataa atacaataac tgtgaataga aaaaatccaa cgatcgatat aaatgttatg
     1261 agacctgctc gaagtaaaac ccgatataat aaaaatgctg tatttcgctt tgctatatct
     1321 agaaaaataa aaagtactgc aatcataatg atagtaaaag ttgtaaaaaa gcacattttt
     1381 tgccctccct ttataaacat cgattctcaa attctaaacc aaatcttgat agttcaagtt
     1441 caggtataac gtataccaac catacatccc aattactatt tatgaactta tgattatcac
     1501 tgaataaatc actccatccc cttgttaaaa cttctacatt aattacacct ggttgttttc
     1561 gactagctaa tccaatgaac ttatcaacca tccttatcca ccaaccacga gaatcttgac
     1621 cagaagtaag aaaaacagtt ctcaaggttg atcgagcagt tccttccagt ttttaattaa
     1681 tctccagtta tagataaata cttgatccta tctatatcag gacttctata ttaagcaaaa
     1741 gtttatgtat aaaactaaaa tcttcattca gttgtctccc cgctttttat acgattgtat
     1801 taaaaaacaa tagaagcctt tcaacattat gttactccag taaagttgga attatagttt
     1861 gacggaagaa aagcaagtat agcttttaaa aaaaactgat acattcgcat ctcctgcttc
     1921 atgtatcagt tttttctttg gattaaactg cttcttcacg cagtttttcc gcaaattcat
     1981 ttattttgcg aattcggtga ttaattcccg attttgatat tgcacctgag ggaatcattt
     2041 cccctaattc tttcaagctg acctctggat gttgaagccg aagttctgcg atttcctgaa
     2101 gtttttcagg aagggcatgt agaccgacct ttgcttcgat caattcgatg ttttcgattt
     2161 gtttagaagc cgcatcaatc gtcttgttca gatttgctgt ctcacaattg actagacgat
     2221 tgaccgaatt tctcatatcc cgtacgattc gaacatcttc aaatcggagc attgagttgg
     2281 ttgcaccaat caatgttaag aaatcagcaa ttttttctgc tccttttaaa tacgaaatat
     2341 agccattgcg ccgttcaagc gttcgagcat gtagaccgta ataatttaac atatcgcaaa
     2401 tgtcttggtt atgctgttca taaatagaaa atatttccaa atgataacga ctcgtttccg
     2461 gattattgac tgaacctgag gccataaaag caccacgtaa gtaagaacgc attttttgcg
     2521 aatttcccat aatctcgtcc gaaacgtgtc cattgaacat cataccatcc atgatatcaa
     2581 gatccatcaa cacatctttt gtgccttgct tcaaccggac gatatagaca ttgttctttt
     2641 ttaatttcat ttttttacga acgagtagct cacttcgtac ctgataatga tctttcaaaa
     2701 gagaatagat acgtctggca attgcagcat tttctgtttg gacgttcaaa ataaactgct
     2761 ggttcacaat acctaaagag ccgttcattc gcagcaaagc agctaattcc gctttcgcat
     2821 gttctcgatg aacttccaaa ccagttagtt ctttcttcac atctgcagca aatgacatcc
     2881 tattctcctc ctttctccaa caataaattg cttaatattt tgaaccgaaa actaaacgga
     2941 acaattcttc tactactttg tctccatcgt ggaagacacc accatctttc aattctaaaa
     3001 agtcggtgga aatcactcga catccttcat ttctcaatcc gttgaagtcg tgtgcgactt
     3061 gtactagata ctcatcatag atctctgggt ccatataatc ttcaggcact ttttccgtat
     3121 tgaccaatac tgtatcgata aaaggctgat tcaaatgctt gtgtaaaaca cgtatatgat
     3181 cggcatctgt gaaatgttcc gtttcccctt tttgggtcat gatattacag atatagacgg
     3241 tttcagcagt tgtcttgagc attgcttcgc cgatttctgg tatcactaaa tttggaagga
     3301 tactggtgaa taaacttccg ggtcccaaaa cgaccatgtc ggcgtccata attgctgaaa
     3361 cgacttttcg agccgctttg gcttcacttt tatcatttgt gttgcagaca tacacacggt
     3421 caatggtttt acggttgatc gcgattttag attctcctac tgcagtcgtt ccgtctttaa
     3481 aaacagcatg caaggtcaaa ggagtttctg aagaaggata aacatgtcca tctacatgca
     3541 tcatttttgc taataattga atcgcctcat aagtgcttcc ccgcatctcg gaaactgcgg
     3601 caatgatcag attaccgatc gtatgattgg ctaaaaactg atcttccttt ttgaaacggt
     3661 attggaaaat atcttcatag aattgtggca tatccgataa cgccactaag acatttctca
     3721 agtcaccagg aggagccata gcgatcgatt ctctgattgc accgctgctt ccaccatcat
     3781 ctgcaacagt cactactgct gtgatatctg ctccttgatt tcgtaaactt ttcaggataa
     3841 ctggcagacc agtgccaccg ccgaccacta cgatctttgg ttttcttatc cggtaggttt
     3901 tcattttcac gaccggttca ccgtctcttt ccttttatcc ttgtcccgat gtgtcacatt
     3961 gactttatag tttttcccta aagcatttgc caaacgttca gtcaatgcca cagaacggtg
     4021 ttgaccgcct gtacagccga ttgcaactgt caagctgctt tttccttctt taatgtatcc
     4081 aggcaataca gtttccagaa gatccaaaaa tttatggtag aatgattgcg tttcatcaaa
     4141 ggacatcaca taatcataga ctggctgatc ttttccggtc aatggtctta gttccgggat
     4201 ataatgaggg ttgggcaaaa aacgcacatc catcacaata tccgaatcga tcggcaaccc
     4261 gtatttaaaa ccaaatgata caaattcgat ccgaaatcct tggtctccct ttgatttgaa
     4321 tgactcattg attttttcac gtaattgtcg tggtgtcaat gttgtcgtat caatgatcac
     4381 ggaagcttgg gctttcaagt cgtctagtat cgcccgttct ttacgtatcc cttcagtgat
     4441 caaaccgtcc atcgccattg gatgtgctcg tcttgtttct ttgtagcgag ccactagttc
     4501 ttcgtctgaa gcatctaaaa ataagataga agtgtcgatg aagttcgtat tttcgatttc
     4561 gatcagcatg ttttggattt cttcaaagaa ggaacgagaa cgcaaatcca ctcctaaagc
     4621 catttttgtg actttacctg attctttgat caattcccag aattttggaa tcaatcgagg
     4681 cggcatatta tcgatacaaa aatagcccat atcttcaaaa ctttggatag ctactgtttt
     4741 ccctgcacca ctcatacctg ttatgatgac taattgcaaa ttatctgtca tatttacgaa
     4801 cccctctcac attgtgcata ataaaattat atcatcccgg gcgtatcatt accagttttc
     4861 tttttctttt acaaaaagat ttggaccaaa tatcttttag gatatttgcc ccaaatccaa
     4921 tttttttact tttccgatgc tgtatccaat gcctcgcttc tttcagctaa tgcacgttcg
     4981 atcgttcgat ccgctaaaat agattgagca tcgatcactg ggtaatgatt atcttcagca
     5041 aattcatgca tcaaagataa ttctgtgcaa cctaggatga ctttttcaca gtttaggcgc
     5101 tctaccgctt cctctaagat ttcgtaatag agctcttgat tcaaataatc cgattccttg
     5161 atctcatgat agatcaggta attgattttt tcttgaagcg cagtatcagg aatcactgtt
     5221 tcgaaaccaa gatttttgac ttcgcgttca tagattccag ctttcatact tccttctgtt
     5281 cctagaatcg ctactctccc tgtcgtatgc tgacggacaa gttcatttgc tgcttctctt
     5341 ggcatatgca agataggaat atccgttgcc gcttgtaact cttcaaaaaa gtagtgtgcg
     5401 gtgttacaag tcaacacaat gaagtttggt cgcaataaat tttgtttctc aatatcatcc
     5461 agcagaaacg gcattggatt ttcttctgat cgatctaaaa tataagctgt acgatcagga
     5521 actgttgcat gattgaataa gacataattc aaatattctt gatcttttgt agctttcgtt
     5581 cggtggttga tcaagcgaac aaaactttct gtcgccatcg tgcccattcc gcctaaaata
     5641 ctgaaaaaat tctccatgaa ctagccttct ttcacatgaa aatacttttt gaatcttgga
     5701 atatagttat ggaacatata tttcatcaac aaccagcgct tgattgatcg gtctttttca
     5761 taaaatacag tagtaccgta acgattttct ttgatcattt gctcagctag ttttttgtct
     5821 tcattttccc gagcatattc taaaaagatt tttttgggca cacccatcca taatcttgct
     5881 ttatctgatt gattcgttcc ataggtcgtt tcaacaaagg gtttgttgaa cactcgatct
     5941 tctgtgacaa aacgagccag attcaagccg ttcaatgtca caaagaaact gctacgacct
     6001 tgtctcaaat tgatttcaaa taatttatat tctccatctc taggatcata cttcatatcg
     6061 aaattggcaa agcctgtata ttcgattttt tccaaaaatg ccttaatggt ttgatagatt
     6121 ttttcgttat aatctggcat gatcacaacg taattaccaa tcgatgctgg tgtaggatct
     6181 tctaatagtg gatgaccaag gcacatcatc cgaacttgat gatcttcatc tacatacgca
     6241 tttagcacac gcatattact atcatcaccc gggataaaat cttgtacaat catttcactt
     6301 ttataaccgg cttcgtatat ccggcctaaa attaaatcga actcttctct agtctctaaa
     6361 ataaaagctt tttttcttcc ttcaaattgg acagaaagat actccacact gtttgcaggt
     6421 ttcaaagcaa ctgggaattc aaaaggcaat tcctgttcca aatgtccatt tactaacatt
     6481 tcttctctga cgatcaatgt tttaggatac ggaagatcat attcttcaca aacttcataa
     6541 aagcttactt tattgatcag cctttcaaat aaagaataat caatatatgg acagataaaa
     6601 tattctgaca gctcttgttt atgttgggag atcaattctg catatccatc accacaagca
     6661 atcaaaagat attttttact cttatcagaa tatctttctt ttgccaaacg aagcatcgtc
     6721 tcgataaata cagggtcttt atcaaatcct ggaataactt ctacattgac aattttactg
     6781 tagcgggtag gggctaactg atccgaagca tatgcttggc agattttgcc gtatgcttca
     6841 ttaaaagaac gtgccatacc ataaacgttc atgtcgcttc ctaataatat agggataaat
     6901 tcttcatttt caatactgtt catcattatc tctcacttac tcataatctc ttgtttttct
     6961 tttccaacta cacaaggaat atcataccat aaagaaaaaa gcaatcaaag gaaagtcctc
     7021 cgattgctca attctttttt gaacggattg aacttatagg aagtttttat ctataccaat
     7081 cagtgtagaa agaactacca tgtaatgatt ctagaacact gatattaata aaacaagcaa
     7141 aaattcattt ttttatgttt ttttaactat ctcagctatc tatcctcttt ataccattat
     7201 ttttttcaaa tagtgacctg tataactttc ttttacgtta atgatttctt ctggtgttcc
     7261 agtcgcaagg atcgttcctc caccttctcc accttctggt cctaaatcaa tgatataatc
     7321 tgctgttttg attacatcta gattgtgttc aatcactaaa actgtgttac cagcatctac
     7381 taatctttgt aatacatgca acaagcgggc gatgtcatcg ctatgaagtc ctgtcgttgg
     7441 ttcatctagt atatagaaat tctttccatt agagattttg tgcaattcac tggcaagttt
     7501 catccgttgt gcctcaccac cggacaatgt cgttgctggt tgccccatcg tcacataacc
     7561 taagccaaca tcaacaatcg tttgcagttt gcgatgaatc tttggaatgt gcttgaagaa
     7621 ttctacagca tcctctactg tcatttccaa aatatcagca atgctttttc ctttgtaatg
     7681 cacttctaat gtttcagaat tatatcgttt accatgacat acttcacaag gaacatagac
     7741 atcaggcaaa aagtgcattt cgatcttaat aattccatcc ccgcgacaag cttcacaacg
     7801 accgcctttt acgttgaaac taaaacgccc tttcttataa ccccgcattt ttgcctcgtt
     7861 cgtttgagca aataacccac ggatatcatc aaatacactt gtataagtcg ctggattact
     7921 tctcggcgtc cggccgattg ggctttgatc gatatcgatg atcttttcga tactttcgta
     7981 gccggaaatt gtcttgaatt tacctggctt agcagaattc ttatttaatt tttgcgctaa
     8041 cgatttcttt aagatactat tgaccaatgt actctttcca gaaccagaca cacctgtgac
     8101 tgctacaaat tctcctaatg gaaattcaac actaatattt ttcagattgt tttctgaagc
     8161 accagtgatc ttgattgctt tcccattacc tttacggcgt tcttttggta cagggattga
     8221 ttttttccct gaaagatatt tccctgtcaa agaattttca tcattagcta cttcttcagg
     8281 agtgcctgca gccacgattt gtccgccttg ttctccggcc ccaggtccga cgtcaatcaa
     8341 ataatctgct gcccacatcg tatcttcatc atgttcgact acaacgaggg tgttgccaag
     8401 atcacgcatt ttctttaatg agccaatcag tcgatcgttg tcgcgttgat gcaatccgat
     8461 cgatggttca tctaaaatat acaaaactcc agaaagattc gatccaattt gtgtagccaa
     8521 tctgattcgc tgcgcttctc ccccagataa tgttccggaa gcgcgactca aagttagata
     8581 gtctagtccc acattttcca agaatgaaag tctgtcattt acttctttta aaatcggttt
     8641 ggcaattact ttctcttgtt cagataacgt taagtttgag aagaaatcta ctgctttttg
     8701 aatagacaac tcatttgttt gcccgatatt cgttccattg atttgaacag atagagcttg
     8761 gggattcaat cgataacctt tacatgtctg acaagttaat tctgtcatat aaagacgcat
     8821 ttgctcacgc gtgaaatcac tgttcgtatc atgataacga cgctcgatat tcttcaatac
     8881 cccttcgaaa gtcgtttcta catcgcgaac gccaccaaaa tcattttcat aatggaaatg
     8941 gaaaagatca tctgtcccat tcaagatgat ctcctgatgc tcttccggta attcttcgaa
     9001 tggtgtatct aaatcgatac cgaaactttc acaagcctgc gctaacattt gaggataata
     9061 ctgagagctg attggattcc atggaacgat agcaccttga ctaagtgtct tcgttctatc
     9121 tggaatcact aaatcttgat cgacttctaa tttgacacct aaaccatcac agtctggaca
     9181 agctccaaaa ggcgcattga atgagaacag tcttggttct agttcgccaa cagtaaatcc
     9241 gcaataagga caggcatagt gttcgctaaa caacatttct tcttcaccga tcacgtcaac
     9301 cagagcatag ccgtctgcta atcgcagggc cgcctctaaa gaatcaaata ggcgagaacg
     9361 aatcccttct ttcacaatga tccggtcgat gacgatcgcg atatcatgtt ttttgttttt
     9421 ctccagctca ggcacttcac ttaaatcata gatctcacca tcgactcgaa cgcggacgta
     9481 tccctctttt tggattcgtt cgaatatttt tttatgttgt ccttttttct ttaccacgat
     9541 cggtgccaac acttggatct ttgtgcgctc aggcaaagcc attacttgat ctgccatttg
     9601 atctactgat tgactggtaa tctcgatatg atcgttggga caaatcggat gtcctacacg
     9661 tgcaaacaac aagcgtaaat agtcattgat ttccgtcaca gtacctactg tcgaccgagg
     9721 atttttactt gttgttttct gatcaataga aatcgccgga ctcaatccgt cgatgctatc
     9781 tacgtccggc ttatccattt gtcctaaaaa ctgacgagca taagcagaaa ggctctctac
     9841 gtatctccgc tgcccttcag cgtacagcgt atcaaatgct agggaacttt ttccagaacc
     9901 ggacaaacct gtaacgacca cgaatttatc tcgtggaatc gttacatcta tatttttcaa
     9961 gttatgggca cgtgcaccat gaatcgtaat tttatcgtta gccatgttgt cctcctactt
    10021 tgaagctttt aattctaaaa tcatatctcg cacattcgcg gctgtttcaa aatccaaagc
    10081 ttttgccgca tctttcattt ctttttctaa tttcatcaat aacgtgtctt tttcttcctt
    10141 cgtcatttct tcgtacgaga cttgtaccgc ttcttttgta tcatcttccg attctttcgt
    10201 aatagaaatc aaatctcgga tttctttaat gatcgttttt ggaacgatcc catgttcttc
    10261 attgtacttc tgctgaatcg tacgacgtct tgacgtttca tccatcgcta gacgcataga
    10321 atctgttact ttgtctgcgt acatgattac tttaccttct tcattccgag cagctcgtcc
    10381 aatcgtttgg actaaggaac gttcactacg taaaaagcct tctttatccg catccaagat
    10441 agcaacaagg gaaacttcag gtacgtctaa tccttcacga agcaaattga ttccaactaa
    10501 cacatcaaat tcacctaatc gaagatctcg aatgatttct gttcgttcca gcgttttgat
    10561 atcactatgc aaatatttga ctttgatccc taattctttg aaataatctg tgaggtcttc
    10621 tgccattttt ttcgtcaatg tcgttacaaa cacccgctgg tctttttcga ctcgttcatt
    10681 tatttcgcct actagatcat cgatttgtcc catgattgga cgaacttcga tcactgggtc
    10741 aagcagccct gtcggacgga tgatttgttg gataacggta tctgtttgtt catgttcata
    10801 tggtcctggc gtcgcagaga cataaatgat ctgattgaca tgtttttcaa actcttctaa
    10861 ccgaagtggc cgattgtcta aagcagatgg caaacggaaa ccgtaatcta ctagcatctg
    10921 ttttctcgct cggtcaccat tatacattcc tctgatttga ggcatggtta cgtgcgattc
    10981 gtccgccacg atcaaaaaat cttcaggaaa gaaatccaat agcgtataag gcggttcacc
    11041 ttctttacgt ccgtccatat gccgagaata attttcaatt cccgacgtgt agcccatttc
    11101 taacatcatt tcaatatcgt aattggttcg ctgttctaaa cgttgagctt ccagaagttt
    11161 gttttcattt cgcaagacag ttaaacgctg ttctaattcc gctttgatat tcgctacggc
    11221 atgctccata tgttcatcat tcgtcacaaa gtgagtcgcc gggaagattg ctacgtgttc
    11281 cgtttctcct aaaacttcac ctgtcagtgc attgacttct cggatacgtt cgacctcatc
    11341 tccaaaaaac tccacacgta aggcacgttc atctcttgaa gccggaaaaa tctccacgac
    11401 atcaccgcgc actcgaaaac gaccgcgctg aaaatctata tcatttcttt cgaattggat
    11461 actgactaag tcacgaatca actgattgcg atctagttct gctccctgtc gaatcgaaac
    11521 tacttgtttt tggtactcaa aaggcgagcc taatccaaaa atacatgaaa cggatgcaat
    11581 gacgatcaca tcatttcttt ctaataatga actagtagcc gaatgtcgta atttgtcgat
    11641 ctcatcattt acacttgaat ctttttcgat atacgtatcg cttgaaggca cataagcttc
    11701 aggctgataa taatcataat aagaaacaaa atattcaaca gcattgttag ggaaaaattc
    11761 cttgaattcc ccgtacagct gaccagccaa tgttttgtta tgggcaataa tcagtgttgg
    11821 tttattcact ttctcaataa gattggaaat cgtatatgtt ttccctgttc cagtcgcacc
    11881 taataagatt tgtgcttttt tcccgccgac tacgccatcc actaattggt taatcgcttc
    11941 aggctgatca ccagccggtt ggtatttcga gacaagctca aagtgtcggg aggtatctct
    12001 ctcaatcatt cttcgtcctc cttttcattc attccttttc attttacgac caataaaaag
    12061 aaaaggaaag caaaggcatt ccttactaaa aaattatacc acaccgaaca tactttcgcc
    12121 acttttttat cttattatac atgttatgtt ttataacata aaatgttaga aactcgcatt
    12181 tttttcattt tttcgagctt acagtcttgt ctgattcagc acttttcgcc tataataata
    12241 aaatattgga aatttaggag gttgattatg aaaagaaagc atttatttca ctcatttgtt
    12301 ttcttaatga caggacttgt gacatttgcg tttggtcatg ttgcacaagc tgacaacact
    12361 acaacggcag agtcaagcac aagcgagaca atgcaaaaaa tagagcccaa aaaagacgta
    12421 tacgtaattg ccagtgactc tgcttttgct ccatttgaat ttcaaaacgc aaagggcgag
    12481 tacgaaggca tcgatgtaaa attgatggaa aacatcgcca aactgcaagg atttaacatc
    12541 cgaatggact accgtggttt ctctgctgct ttgcaagctt tggaatcagg acaagcagac
    12601 ggaatgattg ccggaatgac tgtaacagat gaacgaaaga aaagctatga tttttctgat
    12661 tcttactttg atagcgggat ccaattagct gtcaaaaaag gagatacttc gatcaagtct
    12721 tatgaagatt tgaaaggaaa aacagtcgga gcaaaagttg gtaccgaaag tgctgaattt
    12781 ttaagcgacc atgaatctga atatggctat tctatcaaat attttgatgc tgctgatcaa
    12841 ttgtacgacg ggttaaaagt tggcgcaatc gatgcaatga tggatgatta tccggtaatc
    12901 ggttatgcga tcaaacaagg acaagaattg gaaacaccga tcaaaaaaga atctggcgga
    12961 aaatatgcat ttgccgttaa aaaaggacaa aatcctgagc ttcttaatat gttcaatgaa
    13021 ggattgaaag aattaaaaag aacaggcgaa tacgataaga ttattaaaga atatgtagcg
    13081 gatggttcag aaacttccag tgagtcttcc actgctgatg aatcaacatt tcttggactt
    13141 atccaaaaca actatcaagc attgttgaaa ggactttgga gaacaattct cctaactttg
    13201 atctcctttg ctttggctat agtaatcggt gtgatttttg gattattcag tgtagcacca
    13261 atacgcgctc ttcgcacaat tgctagtatt tatgtcgata tcatccgagg aattccaatg
    13321 atggttcttg cgttctttat tttctttggg ttgcctggta tcgttggatt tacgattcct
    13381 gacttccttg ctggtgttat caccttgact cttaacgcaa gtgcttatat cgcagagatt
    13441 gttcgaggcg ggatcaacgc cgttcctgtc ggacaaatgg aagcttccag aagtttaggt
    13501 ctttcttata atcggacaat gcaaaaaatc attttgccgc aagcgatacg catcatgatc
    13561 ccatcattcg tcaatcagtt cgttatctca ttaaaagaca caacgatcat ttccgcaatc
    13621 ggtgtagtcg aattgctgca aactggtaag atcattgttg cacgtacaac acaaagtacg
    13681 tatgtttact tgatcattgc gatcatgtac ttgattttga tcacagcatt gacaaaatta
    13741 gcaaaagttt tagaaaagaa ggtgaagtaa gatggcagaa aaaatcttag ttgagcattt
    13801 agtcaaaaaa tacggcgata acacagtatt aaacgacatc aatgtatcca tcaacgaagg
    13861 agatgtcgtc tgtatcattg gcccttcagg ttcaggaaaa agtacattct tgcgttgctt
    13921 gaatcgttta gaagaacctt ctgacggtga aattattatt gacggcgccc acttgatgga
    13981 taaaagcaca gatatcaact tggtgagaca acatatcgga atggtattcc aacactttaa
    14041 cctattccct catctttcta ttttggaaaa catcatttta gcaccgatgg acctgaagaa
    14101 agaatctaga gaagaagcag agaaaaaagc aatcaaatta ctgcagacag ttggtctaga
    14161 agataaaaaa gatatgtatc ctgaaatgct ttctggtgga caaaaacagc gtgtggctat
    14221 tgctcgtgct ctagcgatga accctgacat catgcttttt gatgagccta cttctgcact
    14281 cgatcctgaa atggtcggtg atgtcttgaa cgtcatgaaa aagttagcag aacaaggtat
    14341 gacgatggtt atcgttacgc acgaaatggg gttcgctaaa gaagtggcca atcgcgtcat
    14401 gttcattgac gacggaaact ttttagaaga tggaacgcct caacaaatct ttgaaaatcc
    14461 gcaaaatgaa cggacaaaag atttcttgaa taaagtatta aatatttaat aaaaaccaaa
    14521 aatgcacggt cgccatgaag ggaccgtgta tttttatctt cttattttct cacctaatcg
    14581 tatcagccgc atcgcttgtt ttcctgcaca aacaagcaat cgctctacct gttccatcgc
    14641 ataagaaagt accatcggtt cattgatgat atcaagtaca tagtcgatac caccctcact
    14701 taattgagct tgatttttct tctccatatt atgtagttgt gggacaactt tctgacaatc
    14761 agcaattgtg tcaagaacgg atcaatcctt tattggagtt gctcgaaagg gtcttctctt
    14821 attacggaca ggctttgctc actgtacata gacaacaaat cattatccta gtcaatcgga
    14881 tttccagagc ctcattgcta tcattgcttg ataaaatcca gacaaagttt aataaaatgt
    14941 atcagttaca gctaaacttt ggaattgggt cgctttgtta tacagagcaa gagacaccgc
    15001 aatctttcct tcatgccaaa caagtttgcg aatggatagc tttccatcaa tctgtgaacg
    15061 agatccgctt ttttgaagat ttagacctcg ggatcgtttt accagcaatc ccgtcagatc
    15121 aacgaacatt atatgtaaaa cgtattttga aaagcctgac agaagaagaa gtacatttgt
    15181 tcaagaagac tttggcatgc ttcagtaaaa ataacggaag catcaaaaat tgctcagaag
    15241 aattatttat tcataaaaac accttgcaat atcgattgaa caagttccat tctctcactg
    15301 gttatacccc tagaaactat gacgattatc atattctaaa gctggctttt ttgttggtac
    15361 aaacatgaca actacaaaaa aaccaggcga acatatgatt gttcgtctgg tttttaatta
    15421 ttaatgactt agcgttttta ctagaaaaac tacttccgct ttttcccgcc aaaagcatct
    15481 ttaaacttat cgaagaaacc gtcttcttgc tgttcttcaa ctttgatgcc gccagcttca
    15541 gcgaatgcac gtaatgcttc tctttgttgt tcatttagat ttttcggtgt gatcagtttt
    15601 actttgacat gttggtcgcc tgttgcacca ccacgtaact tcggagcgcc ttttccacgt
    15661 aaacggaagt tcgtacctgt ctgtgttcca gcagggatct tcaatttcac atctccatgg
    15721 acagttggta cttggacttc atcccccaaa gctgcttgta caaaacttaa aggcaattca
    15781 tagtaaattt cagaaccttc acgatcaaag atatcacttt cttctacacg gaagaccaca
    15841 tacaaatcac caaatggtcc gccgttttct ccagcttctc cttgaccagc taaacgcatt
    15901 tgttgtcctt cttctacacc agctggtaca ttcactttaa ctgaatgtgc tttcttctca
    15961 tgtcctgagc catgacaggt tggacaaggt tctttgattt ctttccctgt cccatgacaa
    16021 acatcacaag tttgacggct catcactcgt ccaagtggtg tttggcgttc tacattaatc
    16081 gtacctgcac catgacattt atgacaagta gttggttgtg tgcctggttt tgcaccgttc
    16141 ccaccacaag tatgacagat ttcttcccga ttatacttga tttctttttc tacaccaaaa
    16201 atcgcttctt caaaagacaa gtcgaccgta tattgcaaat cagcaccttg acgaggcgca
    16261 tttggatcga ctgaccgacc gcctcctcca aagaaagagt cgaagatatc ttcaaaacca
    16321 ccgaagccgc caccagagaa gccgccgaat ccaccaaatc cgccaccagc tccaccagca
    16381 ccgtaatttg gatctgtgcc tgcatgcccg tattggtcat atgcagcgcg tttttgcggg
    16441 tcacttaaaa tctcatatgc ttctgatact tctttgaatt tttcttccgc atcaggttct
    16501 ttattgatgt caggatggta ttttttcgaa agcttacgat aagctttctt tatttcatct
    16561 tctgatgctc cttttgagag ccctaagact tcataataat cacgttttgt tgccataggc
    16621 tttcctccta ttggttctca tcattttcac gtcgcttgtt atcatatcat attttgtttg
    16681 aaacaaaaac cgatacttca aactattttt caaaagaaaa agccaaagta gcaactttgg
    16741 ccttctctcg aaattcagtg aactgattat ttatcgtcgc catcgacttc ttcaaaatca
    16801 gcatcaacga catcatctgc tccgccttga gctgcttctg ggttttcttg agcttgttgt
    16861 tgtgccgctt gttcgtatag tttaacagtc aagttttgaa cgatttcgtt caatgcatcg
    16921 cgtttagttt tcatttcttc gatgttgttt gcttcaacag cggctttcaa ttcatcgcga
    16981 gctgtttctg cttttttcac ttcttcttcg tccactttgc cttctaattc tttcaatgtt
    17041 ttgtccacag agaatagcaa tgcatctaca tcattacgta ggtcaacttc ctctttacga
    17101 gctttatctg cttctgcatt tgcttctgca tcttttacca tacgttcgat ttcttcgtct
    17161 gttagacctg aagaagattt gatcgtgatt ttttgttctt tttgtgtacc tagatctttc
    17221 gcagatacat ttacaatccc gtttttatca atatcgaatg ttacttcgat ttgtggaata
    17281 ccacgaggag ctgctggaat atctgtcaat tggaatcttc ctagagtttt gttgtcagca
    17341 gccattgggc gttcaccttg taatacatgg atgtctactg ctggttgatt atctgccgca
    17401 gttgagaata cttgtgattt acttgttgga atcgttgtat tgcggtcaat caatttagtg
    17461 aacactccac ccattgtttc gatacctaat gacaatggtg taacgtcaag taaaacaaca
    17521 tctttcacat caccagtgat cacgccacct tggatagcag cacccatagc aactacttca
    17581 tcagggttaa ctgatttgtt tggttctttt cctgtttctt tacgtactgc ttctacaact
    17641 gcagggatac gagtagatcc accgactaaa ataacttcgt cgatttctga ttgtgataaa
    17701 cctgcatctt ttagcgcttg acgtactggt acttttgtac gttctactaa gtcagctgtt
    17761 aattcatcaa attttgcacg tgtcaatgtc atttccaaat gcaatggtcc agattctcct
    17821 gctgtgataa atggcaagct gatttgtgtg cttgatacgc cagataaatc ttttttcgct
    17881 ttttcagcag cgtctttcaa gcgttgtaaa gccattttgt cttgagacaa gtcaatgctg
    17941 ttttcttttt tgaattctgc taccatgtag tcgatgattt tgttatcaaa gtcgtcccca
    18001 cctagatggt tgtcgccagc tgttgacaat acatcaaaga caccgtctcc tagttcaagg
    18061 atagatacgt caaaagtacc gccaccaagg tcgaatacta agattttttc gtcgcgatct
    18121 gttttatcta aaccataggc aagagcagct gcagttggtt cattgacgat acgttctact
    18181 tctagaccag cgatcttacc agcatctttt gttgcttgac gttgtgcatc attaaagtaa
    18241 gctggcacag tgataactgc tttttcgact ttttcaccta aatagtcttc tgcgaatcct
    18301 ttgatatatt gaaggatcat tgctgagatt tcttgtggtg tatatgattt accttctaca
    18361 tctactttgt aacctgcttc accgatatgg cgtttgattg atgaaatcgt atttgggttt
    18421 gtcacagctt gacgttttgc aacttcccct acttggattt ctccattttt gaatgataca
    18481 acagaaggtg ttgtacggtt tccttctggg ttagcgatga tttttgcttc gtttccttcc
    18541 aatactgcta ctgcagagtt agtcgtacct aagtcaatac cgataatttt actcataatt
    18601 aacgtctcct tctttaattg aaagtgtaag ttttatatcc gcacaattat tgtgcaacaa
    18661 tgaccatggt tggtcttaac acgcgatcat gtaatttgta gccttcttgt agtacttcta
    18721 caatcgtatc ggctggtgta tcttcagtcg ctggaacagt ttgtactgct tgatgaagat
    18781 taggatcaaa ggcttctcct ttagcaggga ttttttcaat tccttcttct tccagtgcgt
    18841 tacgcaaact ttccaagacc atttccacgc cttttttcaa acctgcccct tgatcatctg
    18901 acacttctgt ggctaatgca cgttccaggt tgtcaattga tggaagtaat tttttggcta
    18961 aatcttgtga acggtatttt tgtaattgct cacgctcatt ttttcctcga tttgccatat
    19021 ttgcgatttc agcacgagcc cgcaagtatt tatcttccat ttcttctaac tcagctttta
    19081 atttcgctgt ttcgaattct tccgcttcag cttctgaaat acccgaatct tctgcagctt
    19141 cgacatcgat ttccggttct ggctgagcat ctgtcatttc tttatctagt tcttcttgat
    19201 tttctttcac gatacagtct tcctttcatt attcagtcag ctgtttgatt gctcttacta
    19261 agaaagagag aacgatcagc tgcaaatagc ttcgttattc acattacgag tttgacaagt
    19321 caagcgaacg gtagtaatca gcaagtttag ctgctagttc tcttcgataa acatcgacta
    19381 gaccgaacat ccgagaataa ggcatactag taggccctaa caaggctatt gtacctcttc
    19441 catgaccatc tatctcgtaa cttgcttgaa tcatactcat attctgcaat aaatcattgc
    19501 ctaattcagc accaatcttg atatgaatat tgctatccat cggagtgatc atttgtgtaa
    19561 gtccgtctga gtctttcata aaagaaaaca tcgatttgaa ctgatcaaga tcttgacggg
    19621 gctcataatc caagagattc atttgtccac tgacataaac cttatcttca aaaacatgac
    19681 caagaacggt gtcaaataac tgagacatcc cttctgtcgt ttggaaatat ttatgaagaa
    19741 tcatcggaat ctccgtacga agtttatggt acaccgtcat cagaggatct ccgactagac
    19801 ggtcgtttac aatctgtacc attttttcta gttcctgact tcccatatat tcgggaagag
    19861 tgaacacttg gctttctaca ttccccatat ctgttacgac gatagcaata atctgtcgat
    19921 tattcaaggg tacgatacga aagccagtca gtcggcggtc ttttatttca ggtcccaatg
    19981 aaaaagtcgt atagcttgtc agctcagata atatctcagc agattgctta ataatctcat
    20041 tgatttcatg aaattctctg cctagagact gacgaatcag cagcaaatca tcttctgcta
    20101 cgcgagcagg ttttaacaaa tgatccacgt aataacgata gcctaaagca gatggaatcc
    20161 gcccagaaga agagtgagtc ttcaacagca atccttcatc ttctaatgct ttcatatcat
    20221 tgcgaatcgt tgctgagctg gccttgactc cctcagacat caatgttttg gaaccaacgg
    20281 gcacgccact actcgtatag tgttggatga tcagacgtaa aatgtcgttt tgtcgctttg
    20341 ttaacatctt cctcacctct cattagcact catatattct aagtgctaat ccacttataa
    20401 atatagcaag aagcccttct cctgtcaaga gaaagactcc attttttagc actctaaaca
    20461 caagagtgct aattctaaat aattgatgta aatccagtta tatcaacgtt tcaatagtcc
    20521 tttattgtat ttttgaactt tttttattta aattcagata aaaaacaaat attctttaaa
    20581 ctatttttca aacaaaaagc gctcaaacac attgttcccc aaaaaggtcc ctcttggtgt
    20641 caaataaaca cggtcttctt cattaacaag taacccttct tgctctaatt cccttagcac
    20701 atcaccgtac accgattcaa ttgttttttg atagcgatca tagaaatgtt ttttagtaat
    20761 ccctgttttt tttcttagtc ctaagaacat ttcttcttct atttgatttt ttcgtgtcaa
    20821 tttttcttct tccaataccg ggcgttcatt tgctcgtaat gggcgtaaat agtgttggat
    20881 tggtcctttg ttgcggtaac gaatatctcc aagataaccg ctagcaccag ctccaaaccc
    20941 aaaatagtgg tcattgttcc agtacatgag gttatgttga gattcatgtc cggagagccc
    21001 aaagttactt atttcatatt ggtgacgtcc atgcttttcc attgcttcaa tcgtctcttc
    21061 aaacattctg gtttccgttt cctgatcagg cagttcgagt cttccttgtc gtacccagtt
    21121 catgaacatt gttttgtttt cgaggatcaa tgagtacaaa gaatagtgag gcagatccaa
    21181 agccaaagct ttttccaaag tatccagata tccttctagt gtttgtcccg gtaatgcata
    21241 aatcaaatcg atgcttacat tggtaaaatt ttctgcttct aaaaattgca tagtttgata
    21301 cacatcttct gcggtgtgtt tacgtccaat ttttttcaat aagcgattat caaatgtctg
    21361 cactcccatg gataaacgat tgacgccata gtttttcatt acttgaagtt tttcacgggt
    21421 caaatcacct ggattcgctt ctacagtaaa ctcattacca ttttgaaaag gcaggatctc
    21481 tctagcacct gataacagtc gatcaagttg tttagcagat aaagaagttg gtgtcccgcc
    21541 ccctacgtaa agtgtttcta aattcgctgt cggatgactt tctagcatta ttttcatttc
    21601 attcagcagc atttccacat actcatctac tggctgccct tctaaaaaaa ctttattaaa
    21661 atcacagtaa tagcagatat gctcgcaaaa tgggatatga atgtacgcag aaaccttgtt
    21721 ttctgcggga tttattgttt ctatcattga aattctttct ccttacgtcg aaaagtcgtg
    21781 cctgttttcg accttcttaa aatcgcgttc aagcaccaac tcattgaaat aagccgcata
    21841 tttagaatca aggaaaccta gctctaaaaa tgcttgctta ttgtcatcgg agctacacgg
    21901 tgcttttaca gcctctcgat aaacggtaat cgcaaagctg accttcggca ttaagtcaaa
    21961 atctgcaaaa atatgaaaag ctattttcac agattcctcc ttaagtgctc agaccacaac
    22021 gctttgctta caacctctca tatacgcata ctaaacgtat ttggcgaaca aatccactaa
    22081 aaaatccaag aatcgacttc ttggatgatt ttttctttca ttaaactggt tacctagtta
    22141 tatatctcta gggttgtttt catgtctgag tgcccgatat gatttatttt tcgtttctat
    22201 aattactttt tgccttccag tttcttttaa tattgtactc aagcaagtgg gaattggttc
    22261 ttttgtcatc catgtatgca aaatgttata atgctggtaa acaaaaatga ttacctgtca
    22321 tttttattta gttcgtattt aattaagttg actatctgta tactctgtct aaaacttaag
    22381 aattgttgtt ttcgctgtcg ctacccaatt ttaatgaatg tctgtaggtt aaaactgttg
    22441 tccttgttat agatagtaaa ggagagcttg aaaatgaaaa ctgcgattac gtttctatta
    22501 gaggaatatg ccgattggga aggtgcttat attactagca cactaaacca aaatgagaaa
    22561 tggcaaaaca aaactgcttc tatccaatca gaggtcactt ccattggagg attaaaaaca
    22621 aaaatcgatt acttaatcga acaaatacct gaaaaagttg acctttttat tatgatcggt
    22681 ggcaattcat gggacataaa aaatcatcat ttatatcaaa taatcgataa attgttaaaa
    22741 acagacgtac cagtagctgc tatttgtggg gcagttgatt atttagctca gagcggatta
    22801 ttgcaaggtt acaaacacac cggaaatgcg caatttcctt ggttagaatc ggaaaattat
    22861 cagaataaag aagattttat agaacaacaa gctgtgaaag atcgtaattt gattactgcg
    22921 aatggaacag ctcctataga attcacagac ttgattcttc aagcagtcca ttttgaaaca
    22981 gaagaaataa gagaaaaaac gattcgtcta tacaaacttg gattctatga ttactataaa
    23041 aagtacggag atcctttctc ctaatcattc tcttcaatta aatactcctt atcaatagtg
    23101 tgacaagtaa ttttactgat gattatttgt cacacttttt ctgacattta tcaaagaaga
    23161 tacactctat ttatattcat agacaaggtt ttacggattc aaaaattcag gattatgcta
    23221 tactggtatt aaaaaaataa tgaggtattt tatatgaaaa agttaggttt tattttattg
    23281 ttggtctctt tattccttgt aggatgtacg tctcctagta aagaaaatag tgatccatct
    23341 gcgaccacag acaaaagttc aatcagacaa accagttcag ctgaaaaatc aagtactcag
    23401 aaatcagctt ctactgaaac gaatacatct tcaacaagca gtaaagtctc agagcccaat
    23461 acttcttcta catctagtag tacagttgag acaaatattt cttcttcaac aatagacaca
    23521 acctcctcac agtcttctca gtcaatagat ccgttagcag gatattctga tctacaaatt
    23581 gagtacgcac gtgtttggct agctattaga ggtaatactt ttagagatac atcgggtgat
    23641 ttcgaattac atgttattca ttatcctgca ggaacaccta tcgcccctta tgatgcaggg
    23701 agtgcagtct atccgaaaga tgtggttatg ctcactggga aatacagtta ccagggagtt
    23761 ttagtgtatt caagtaacca tgacgggaca atcactagtt atccagttcc ttcacattgg
    23821 caaatgcctg cagatcaaac atcttatcca gcgttcatta gagaaacaac tcaagaaatc
    23881 attgataatg catctgtagt agctattcct acaggaaatc ccaatgacgt gaaacagtta
    23941 atcgatgtga cagttataga taattagact ttctaatatg ttatacaaaa agaagctgac
    24001 agcattttgt cagcttcttt cactttcttt tctatttaga tttattggtc tttgatagac
    24061 tccaaggcag gaaaaaattt tttaaataaa cgtttgcaaa acagtcgctt ttctatcttc
    24121 taccgctact tctgcatagg caccattaat caatgtcagt tgtggcggta catgaccaca
    24181 atcaatatca tacaagatcg gtagttttaa ttcctttgac agatcttcgt atacatcctt
    24241 cattttataa tctaacaaat cctctgccgc acttctaccg aacatgatcc ccgagctgcc
    24301 atcaaaccag cctgccagtt tcatctgaac aagtgatctt cttaaatcaa ctgacgatag
    24361 ctcacagttt tctagaaacc acaagatcgg ctttccatca ttatactttc ttttaaaaga
    24421 ttggacatct ccataaggag taccgattaa atgcctgatc gtatcaatac aaccacctaa
    24481 taatcgtccc ttcatcctta ctgcatctcc gctgactgat tgccaatttg ttggttcagt
    24541 gagatggaaa acagaagatg atgaatgctc atggggccac actttttgat acttttcaga
    24601 agattgctgt agaatcgatt ctccttctct tgttttcagt acactttccc acattgctgt
    24661 cgtttcatcc aagtactctc ctcttaaatc aataaggtta gttccatgtg cagtggctat
    24721 ccctgttttt aatgtgacag caagtagcaa cgtactaata tctgaataac ctactatcca
    24781 ttttggttgt agagcatcaa aatcgacaaa atcgagaatc tcaatcaata attctcctcc
    24841 ccaagtcgga aagatcattt ggatattttc atcttgcatc atttgattga attcatctgc
    24901 tcgttttctt gctggacctg atttttcctt atcttgcgtc caaagcgtct ttccagtaac
    24961 tacttggaaa gatttttctt ccattctctt taaagctgat tcgagtaacg aatggtgttc
    25021 ctctgatacg cctgaggatg gtgcagtaac cccaatcgtt gcatttcttt ctaataatgg
    25081 ataacttatc atgttgcttc acctccaagt atgcactatt ttacttggtt aattgttttt
    25141 ttcgtttgcc ggcatcaaaa atatcggcat ctcaagtagc tggatgattt tgtttaagtc
    25201 aaccgtttgg atgaccatcg tttgatctgg tctacctggt gcaaaaacaa gtttttcatt
    25261 tgataagagc tcttcatcaa taataatcgg aatctgtttt tcataaccaa acggagaaac
    25321 agcgccctgc ttttgtcccg tttgttcttc catgcgttct gatgaaacaa tagaaagcct
    25381 tttaccgacg atcttcccta atgctttgaa atccgtttgt ttcgtagtaa aagtcagata
    25441 tacgtagtta ttatcttttt tatcttttaa gaataagctt ttggtctctg tacctgtaaa
    25501 tccttgttct ttctttatta ccaaagcatc ttcatttgta taaatggctc gatgatgata
    25561 caactcaaaa tatatatttt cgtgtaagaa tttctttgcc aagtcaatca tttttatgtc
    25621 tcttttctat tttattattt gagacatctt atcatataaa tatagcggtt acaacattac
    25681 ttgtgttttg ttcgtttttt atacataact attgtttttc tggtaaaata aatcaaacca
    25741 cttaatggag gcgttttaga atggaaaaat ttcagccgat catcaatcaa atcaaagaaa
    25801 aagaacatca acaaaagatc aaagaccttt ttgctaagat aacagaaacg tatccccagc
    25861 tagacacaac tgtaaaatgg aaccaaccta tgtttactga ccatggtacc tttattattg
    25921 catttagttt tgctaaaaat catttttcta tcgcaccgga aaaagcagcg attcgtgccc
    25981 ttgaaaaaaa tatccaagaa gcaggctacg tatatacgga taacgtcatc aaagttcctt
    26041 ggaaaagtgt aatcaactgg gaattgattg aacaattgat ttcatttaat attgaagata
    26101 aaaaaggtca tgataaattt tggcgataaa gctttatacg aataaacctt ttactggcga
    26161 ggaaactatt catcacctta tacaggtttt gttcaatcct atccttttta tcatccctgg
    26221 ttaaacactt aattgacaaa gtatctgcta tttttataag gagtctttct tgtgctgaaa
    26281 aaaatcactg attgaatggc tttgaatttt ctacatagta aaattatttt ttcagcaatt
    26341 ttcctgcaaa acttttgaat aaggaaaaaa tacgagaaaa tatcagtgat ctttcataat
    26401 actgcccatt attttcaggt atatttaaat ataatatcac tttaggagag aacgaatttc
    26461 tttagagaaa taaaagttag aattttctga cttgattttt acaatgacct taattaaaat
    26521 cgttctttct acatttcgtg attaagcaaa tatcaactaa gtcagtagaa ccattcacat
    26581 aggagggtga acattgaaag atttttactc agtaaatgaa ttagccgaac aattaggtgt
    26641 tactacacgt tctatcagaa attatcttca tgaaggaaaa ctaaaaggaa caaaggttgg
    26701 cgggcaatgg aaattttctg aacgtaatct ttttgagttc ctatacggag atcaagcaga
    26761 ggaagcagca aaagaaatgc agcgattcat gctaaatgca ccaatcacga tgcgtttcaa
    26821 ccttcaatat cgtgatttta cagcaatcaa tcaattccga gaacaattag tccagtacca
    26881 taacgatgtt tacgcaaata aaaaagaccg tcttttacaa tacgatcttt ataaagacaa
    26941 tcatgcggag atactaatcg gtggtaactt caattatgtc gtaaacttta gtcaatggat
    27001 caatgaaaaa ttattgatcc agacagatat ttcccttgta tcttaaacaa ttaaaaagcg
    27061 ctagaaattc cagagatgtt ggaaattcta gcgctatttt tttataagta cttactgttt
    27121 ggttcaaaca aatatttttt taataaatga aattttacca tctttatcct caaataaaga
    27181 ccgtgaccat gcttcctact acgatcagca ataatccgat aatcgttaat tttttttctt
    27241 ttggtgtctt attttcatgc aggatgtaca taccgcctaa tgtagagata actacgttta
    27301 gctgtgtaat gatatatgca gtagcaaccc cattttcatt tgctgaaaaa atataagcca
    27361 atgctgacac actaaagatg gctccaacga tcgttgcttt ccaactcttg tcttcagaaa
    27421 atgcggttgg tttacggaaa atcacataaa tcactgcccc taaaaagaca ccgatcattt
    27481 gcggaaagaa aatggaaagt ccgcttgcat caactagttt aggcaatgca ctgtataccc
    27541 aataaccaat acttgttgat gctaatagac taattccttt tgtgataccg cctttttttc
    27601 caggatcatc actgattgcc gttaaagcag aaccaaatac aagaagaatg attgctgctg
    27661 ctccaatgat tttccccata cttccagacc attctccaaa agcgaagaca ccaatcaacg
    27721 aggttccgac taactgtaat gcagtcgaaa ttggcatcgt tttagaaaca ccaatcaagt
    27781 tgaaagatgt atactgtcct gtttgtccaa taacccaaaa tgcaccagaa agtaaactaa
    27841 tcaaaaatgt agtcactgaa ataccagctg gagaagcgaa taattgaacg agtaagccga
    27901 caagcaccgc tccaatacca gttcctaata tctcatttgt tggtctgcca ccaatctttt
    27961 tcaaaatcag tggctggatc ccccagccaa ctgccgggat caatgcaatc aataaaccca
    28021 tttttattca cccttatcct tttccacctt ggaaagaagg atataaagtc atcccaccat
    28081 ctgcaaatag tgtaatacct gtaacataac tggattcgtc tgaagccaac catgcagctg
    28141 tcgccgctat ttcttttggt tcacctacgc gtcctagtgg gatcatactt tccaagagtt
    28201 ctttttgttc aggatcatca aattttgccg cattaatagg tgtacggata gcaccagggc
    28261 ctatactatt gattctgatt cccttcggtg catactccat tgctaccgtt tcggtaaata
    28321 attttacccc gccttttgaa gcagcgtaat gagcaaacgt tggccatggg atctgttcat
    28381 gaacagaaga catattgatg atatttcctt ttttattatt ctctgtaaag taatttaaag
    28441 ccgctttcgt tccgataaaa acgccagtca aattgacttt taacactctt tcccaatctt
    28501 caaggctaag ttcatgggtt ggatgctgat tttccatccc agcattatta acccaaacgt
    28561 ccagtgtccc aaaattttct aaagccgtat caagtaaacg ctgtattcct tcttcagaac
    28621 tgacatctgc ttgtacagag actgcttttc cgcctgcctc ttcgattgct ttgactgctt
    28681 tttctgctcc ttctttatcc gagttatagt tgacaactac attcattttt tctttcccga
    28741 atcgttgagc aatcgctgtt ccaatcccct ttgagccacc agttactact gcgactttgt
    28801 tttctaattc cttgtacata gtacccactc ctttttcata cctaaacctg ttattacgga
    28861 taacttttat ttccgcctta aagtgagtgt acaccaagat atatagtcta aagcaaatga
    28921 tacgctctag caatcgtttt catgaattgc tagtgtcctt ttatttctat aaagaaagag
    28981 gttgtgacag aagtggtcag cttcaagaaa tttcggctta tttccgaaga agctacttct
    29041 gaaaaaccat ttatttggaa aaaaaggctg tgacaagact tttgtcacag cccctttcct
    29101 gggcttattt atcacccatt ttattgtaat tgctagttat acataaacaa gagattttat
    29161 gcataatgtt ttattgctga gaaaaatatt cagctgttgc ttgttcatcc tccttgagtt
    29221 gttcaatcaa ttcctctaca ctagaaaatt tcacttgatc tcttaaaaat tgattccagc
    29281 gtaccgcgac atgttcacca tagatatctt gattaaagtc caaaatatac aactctacag
    29341 tcagctcccg tccttcaccg aatgtatcat tatgtccaat tgaacccatg gctggatacc
    29401 atgtttcccc aatttttatt tccgacacat aaacaccttc tttaggcaaa tgaaccgtgc
    29461 tctttacttt gacatttgct gtagggaatc ctagtagtct gcctcgtgca tcgccatgga
    29521 caactacccc ttctacctca tatgtgcaac ctaatagttt tgctgcttct tttacttgtc
    29581 cgctgtctag taatccacga atacgggtag agctgatttt ttcaccttgt tcttcttctt
    29641 tagaaactgt gacgattttg aatcttcctt tggcatattc aggaaaatga gcgacatctg
    29701 caatgtcttt tggtccatac gtataatcaa aaccggaaac agcatatttt gcatgtaacc
    29761 ctacgatgta ttgatcaaca aattcctgcg gtgctaaatg agcaaaagaa gaagtaaaat
    29821 caacttcata taaataatct atacccaaag cggccatttt ttcttctttt tgcttaaggc
    29881 tagtcaaata tttcacgctg ctaggatcga tctttttaaa gacaatagat ggatgctgat
    29941 tgaaagtcat caatgctaat ttcaatcctt ctttttgtgc aatttccata cctgtcttta
    30001 tgactttctg gtgtccgata tgcacgccgt cgaaaaatcc cagcactaaa acgacatcgt
    30061 cttctggaat ttgttctgat tggtaaggat gtctgatctt gatgatattc attcgttatt
    30121 cctcatttct caatacttta ctaggtttta atttgttttt ttctttcggg tttggctgat
    30181 aaatactgac aacttttcct ttataaaaca aagcaatttc ttctgacggc atttctggga
    30241 gcgcaaatac agtataatca agtctcatac cgttttttac tttctgccat acttcatcag
    30301 taatatctac tcgtttgaac cgttccactc ctttttctat tggcagcaaa caatcatcga
    30361 ttgtcccatt tttcacagct tgagctactt cttctaatgt cagagcatcc ttctgggaca
    30421 taccggcact tgctgttcgg gtcagatcag acatgtgcgc agggtaacca agtttcttgc
    30481 ctgtatctac agctaatgtg cggacatagg ttcctttccc gcattctact tcgaagcgcc
    30541 aagaaatggt tcctgtcact tcgtctcttg tcaattcact tgtccggata aaacggtaaa
    30601 tctgagcctt tctaacagga cgctccactg tttcattatt gcgggcatat tcatataacc
    30661 gcttaccgtt gacttttacc gctgagtaca ttggtggtat ctgggtgatc tcaccaataa
    30721 atgaagccat cacttcatct attttttttt cagataaaga catatctact ggttttgttt
    30781 ctacccgttc tccggatcga tcttccgtcg ttgtactgaa acctagagtt atctcgccta
    30841 catagatttt cccagaatct gtcaaatatt cgataacttt tgtggctttt ccaatacaaa
    30901 tgggcaacac accgtctacg tctgggtcaa gcgtaccccc atgtccgatt tttttcgtat
    30961 gtaagatttt tcttaatttg aatacgcaat catgacttgt cattccgcgt tctttccata
    31021 aaggtaataa cccgtccatg tcttttctcc atttcatttt tgatttaact gaattattat
    31081 aacacattgc atcaattact gacgaatttg acatcataat gaacggtttt tatttttcgg
    31141 caataaaaac gtaacaaaat tcatgctcac gtaaatagaa atcatgttat gcttttttaa
    31201 aggagggaat agaatggaag attttttctt tgcaccattt gattggattt atgggtggct
    31261 tacaggtaaa tagtttggtt ttcacttccc aaaaacgaaa aaaaagactg gccattcttc
    31321 cagtcttttt tccgtttttt ttatttattt tttcagtttc ttaatcaatg taaaaaattc
    31381 tattttgcgg gatcgatttt atcattttat tggtcaagat ttttccattt ccaatccaat
    31441 acttctggca gatcaatacc attttcacga atatagtttt gatgttcttg taatttctga
    31501 tccatttttt tcgcaaaatc ttctgcttga cttcctttga tttttaaagc tgcgtctttt
    31561 gccaaatgat aacgatcaag ttcatttact acacgcatgt caaatggtgt cgtgatatct
    31621 ccattttctt tgtagccatg aatcattaaa tgatggttat gacgatcaaa gaaaatcgtg
    31681 cggaccaatg tttcataacc gtggaaagca aataagatcg gtttgtcttt tgtaaaatat
    31741 tgatcaaatt cttcatcgct caacccacgc ggatcttgac ttggatgacg taatttcaat
    31801 aaatctacga cattgatgaa ccgaattttt agttttggga attcttccag cagcaaagtt
    31861 acagctgcta atgcttctaa gttagattcc gttcctgcag cagcaatgac caattcaggt
    31921 tcctcctctt ttgctgtcga agcccaatca atgattttca agccttcatt cactaagacg
    31981 gctgcttctt ctgctgagta aaattgagca cgcggatgtt tggaagagat gatcaaattg
    32041 attttttctg acgagcggaa tgctttatcc atgactgcga gcaatgtatt tgcatccgca
    32101 ggcagatatt cacggatgaa ctccgccttc ttttctgcca aatgtgtcaa tattcctgga
    32161 tcttggtggc tatatccatt gtgatcttgc tggaagactg ttgagcttgc aatgagattt
    32221 agcgaaggat aatcatttcg ccaagaaagg tcatgtgatt tccgcatcca tttgaagtgc
    32281 tgagtcaaca tagagtcaac cactcgcaag aatgattcgt aacttgcaaa aaaccatgcc
    32341 gtccagtcag aacgtaccct tctaagaacc cttcatcttg gtgttccgat aattgactgt
    32401 cgatgacgcg accagatggc gaaagccatt catcattttc tggttcgatt ttttccatcc
    32461 attggcgatt tgtcgttttg aaaacttgat tcaatcggtt tgattttgtt tcatctggtc
    32521 caaatatacg gaaattttct ggatttttct caatgatctc gcgaacgaat ttaccaagtt
    32581 cggtcatatc ttctgcttta acggagcctg gtttttcaaa ttgcaaagta tattttttcc
    32641 aatctggcat aataagcggt cttggatcga ttccaccatt ggtgatagga ttttttgcca
    32701 ttctttggtc accagtaggc gcgatttctc tgatttcttc taaaatacgt ccttgtgcat
    32761 caaataattt ttctggttca taggatttca gccaacggag taaagcatct gcatgatcca
    32821 tatgttcttg atctacagga atcggtactt ggtgtgctcg gaaggttcct tcaattggtt
    32881 ccccgtccca ttcttttgga ccggtccatc cttttggtgt gcgtacgatc aatactggcc
    32941 attttggcat cacgacatct gttgctgtct tcaggcgagc ttctttttgg atggatttaa
    33001 ttttctcgat tgcttgatcc attttttcag ccataacagg atttaatttt tctgaatcat
    33061 ttccttcaat aaagaaaggt tcccagccaa gtccgttaaa gtaatttgct aattctccat
    33121 ctgatttacg tgccaagatc gttgggttgg ctatttttgc accatttaaa tgaaggatcg
    33181 gtaaaacagc tccatctatc actggattga taaagacatt tgagaaccag cttccagcta
    33241 aaggaccggt ttctgcttca ccatcaccaa tcacgacagc agagattact tcaggattgt
    33301 ctaatacagc tccgactgca tgtgaaagag agtatccaag ctctccgcct tcatggatag
    33361 aacctggtgt ttctggtgct gcatgagaag caatgcctcc aggaaatgaa aatcgtttga
    33421 ataatttttg cattcctgtc tcatcttccg ttacttctgg atagatctct gtgtaacttc
    33481 catctaaata agcattcgat accatgactt gtccgccgtg tcctggccct tcaatataaa
    33541 acatgttcaa atcatattta ttgatgactc ggttcaaatg gacataaata aaattttggc
    33601 caggaatcgt tccccaatga ccgattggat gttttttgac atcttcgggt ttcagtgtac
    33661 gtctcagtaa ggggttatct ttaagataaa gttgcccaac agaaagataa ttagcagcac
    33721 gccaccaagc cgtcatttta tcaaagtatt ctttactaga ataatccata cataacactc
    33781 cttttcatta ttactcttat tctagcgcag atactcccaa ttgaaaccta ataagcactt
    33841 ctgttcattt gtttggattt tatatctagc caaatgaaca ggtataaaaa accacgaaat
    33901 ttttcctttc gctgacgttt tttacattga tccaaaaaac ttgtcgttat cacaatggct
    33961 gttccgcagt tcatttcgct cctcatcatg cataatttgc tgaatgacgc tgggccagtt
    34021 aacacattgc tggaaaactg gggctggatc gagcaattcc tttcctgatt gatccgtttt
    34081 atgtaaaagt ctctgttatc tttgtcaata tgtgaattgg tatcccagct actatgctaa
    34141 tttctacagg gattatccaa aatttggatc gtgaccaaat cgaagcggcg taatcggaaa
    34201 tatcaataac ttcaacgtga tttttctttt aactgtcaac ggaccgacaa actcaaactt
    34261 ttatggtgca ggttccactg atcttctagt tacttggcta tacctgactg tcactaaaat
    34321 agattacaat ctagcttccg ttatcggtat cttgatcttt atctatatgc aaaaatttat
    34381 atactactag aaaaaagaaa gtcgaatttt ttacttttct ttttctcttt tttggttagc
    34441 tgacacgccg caagactaaa gtcatggaac tcttaggtaa tacgtcttaa tgaaaacaaa
    34501 aaaatcccgc aacccctgat ttcacaggat ttgcgggagt ttttgattag tctttgttca
    34561 agtcgcgaag caattcatcg atatggttgc catagacaac tgattcatca agttcaaaga
    34621 acaattcagg tgtcttataa atacttagat tgtgccctaa ctctcgacga atcaatcctg
    34681 atgctttatt caatccatct tgtgctgttt tcttatctga tgctaagtca gataaaatgc
    34741 tgtaatagat cgttgcttgc tgtaagtccc ccgtaacttt cacatctgtg atcgttacgt
    34801 tctccacacg agggtcacgg acctttttgc gcaaaatatc attgacttct tttaagattt
    34861 cttgaccgac tcggcggtca cggtagttag ccataatgat tggcctcctt ttatcagttt
    34921 ttagtcattt ttgacttctt ccatgataaa tccttcaatt acgtcatcta ctttgatgtc
    34981 attgaatttt tcaatcattg caccacattc ataacccatt ttgacttctt tcacgtcatc
    35041 tttgaatcgt ttcagacttg caagttggcc ttcatagatt acgattccgt cacggattac
    35101 acggacaccg ctatctctac gaatatatcc ttctgttaca taggcacctg caatcgtacc
    35161 aactttagaa actttgaagg tttcacgaac gatcatttga ccggtgattt tttcttcaaa
    35221 ttcagggtct agcatccctt tcatcgcagt ttcgatttct tcgatggctt tatagatgat
    35281 acggtgaaga cgaatatcga cttcttcagt atcagcttgc acttttgcct gcggtgtagg
    35341 acgaacgttg aaaccgatga taatagcgtt gctggctgct gctaaggtta catcactttc
    35401 attgattgct ccgacagctg aatggacgat ttttacacgg acaccttcca catcaatctt
    35461 cttcaatgat gcagccagtg cttctgctga accttgtaca tctgctttga tgattacgtt
    35521 aacttctttc aattcgcctt ctttcaggct ttcaaataag ttatccaaag tcacacggct
    35581 tgtggcagca cggttttcta ataatgcacg tttcgcgcgt tcttcacccg ctgcacgtgc
    35641 tgtcttttcg tcttcgaaga ctacgaaacg gtcacctgct tgaggtacat cattcaagcc
    35701 agtgatttct actggcgttg caggtcctgc ttctttatcg cgacggccaa gatcgtttgt
    35761 cattacacgg acacgtccgt aagtattacc gacaacgatt gggtcaccaa catgaagtgt
    35821 tccttcttgg acaagtaagg ttgacacagg acctttacct ttatcaagac gtgcttcgat
    35881 cactgttccg attgcgcgtt gtgttggatc tgcttttaaa tcttctactt ctgcgactaa
    35941 caggatcatt tccagcaatt cttcgatgtt ttggccgaat ttcgctgaga tctcaacaaa
    36001 gatcgtttct ccaccccatg cttcaggaat taactcatac tcacttaatt cttgcataac
    36061 atgttgaggg tttgcacctg gtttatcaat tttatttaca gcgacgatga ttggaacgcc
    36121 tgcagctttg gcatggttga ttgcttcaac tgtttgtggc atgacgccat catctgcagc
    36181 aacaactaag atcgtgatat ctgtgatact tgctccacgt gcacgcatac ttgtaaaggc
    36241 cgcatgtcct ggtgtatcca agaaagtgat tggtttacca tcaatatcga tctggtaggc
    36301 accgatatgc tgtgtgattc cgccggcttc gccgcttgtt acacgtgaat ggcgtaatgt
    36361 atccaataaa gttgttttcc catggtctac gtgtcccata atcgttacga ctggcggacg
    36421 tgagactaat ttatctggat cgacttcttc tgcctcgaag aatttatcga tatcagcaat
    36481 gtcaacttgg actttttctt gtggttccat gccatagtcc gtcgctaaca gttcaatcgt
    36541 atctttatcc agtgcttggt tttgattgac catgacacca agcataaata attttttaat
    36601 gatttctgct ggctcacgat gaattttttt cgcaatatct gctacgttca taccttcagt
    36661 atactctaat acatctggca attcacggaa tttgcgtggt ggtacagctg gtttagttga
    36721 ttgttgctgt ttgccttttt tacctttttt attataacga ttacggttat tattattaaa
    36781 tttgccgcga ttgttattgt ttggtttgtt gttattgtta gtacggtttt gattgccgcc
    36841 ttggctcgtt ccttgacgat tttggctgct ggtatttccc gtattatttt ggtttgttgt
    36901 tggttttttt ccttgattca cttgaccgct cccttgaccg cgatcttgat aactgcggtt
    36961 atttttattt ttttgttgat tgttcgttgt ttctttttta cgttgatcgt tcggctgttg
    37021 attggctggt tttctgcttg cttgttgtgc agcttgttgt ttcgtgcttg ggtttttgaa
    37081 cgcttgttgc aatttcttct catcattgct agtaatcgca cccatatggt ttttcacatc
    37141 gatgccaagt tgttgtgctt tttccacgac gtctttacta gattttttga gttcttttgc
    37201 taattcatag attcttttgt tgcccatgca atcaccttcc taaccttcaa ttagctcctt
    37261 ggtcttcttc gcaaatccgg catccattat tccgaatacc ttgcgaggtt ttccgatcat
    37321 ctgcgtgatt tctgcttcag aaaatagctc aaagcaagga acttcataat atgaactctt
    37381 atcttttatc ttttttcttg tattctcgct tgcatcggtt gccacaaaca ccaattttgc
    37441 tttttgtttg cggatttcag caatggtcag ttcttcaccc gtgattagtt ttcccgcgcg
    37501 catcgctaga ccgatgagat tcatggcttt ttgccgatta actgtattca ttttccaaat
    37561 aactctttac gtgctttttg gtgagtgaca tagtcaagaa gttcttgata aaactcatct
    37621 gttaatgttg tttcgagtac tcgatcgaga atttttttat cccaagcttg ttgtacttct
    37681 tccggctcaa gtgagacata cgctcctcgt ccaggcattt ttccagttgg atcgatcgat
    37741 acttcccctt cttttgaacg ggtgattcgg accatctctt ttttaggttt catttcacct
    37801 gaaaccacgg atttgcgtaa aggaattttc ctttgtttca tctctttgcc tccttactaa
    37861 agttaagctt ctggtttttc ttcgaccgtt tcattgttga atgcaattgt ttcatattca
    37921 tcgtctgtta aatctgactg aacgattgct tcatcatgca gttcttctgt ttctttttgt
    37981 tgttcttgat tttcataaaa ttccgtcatg tcagattctg atttgatatc gattttatgg
    38041 ttagtcaatt tagcagctaa acgtgcgttt tgtccgcgtt taccgattgc tagcgataat
    38101 tggtagtctg gaacgacaac tgtgcaggcc tttggatttg tctcatcgaa gatcacatcc
    38161 atgacttgtg caggattcaa tgcatttgca atgaatacag ctggatcttc attccattcg
    38221 acgatatcca tgttttcgcc ttttaattca ttgacgattg cttgaactcg ttgtcccttt
    38281 ggcccgacac atgtaccgac tgcatcgata tttggatcat ttgagcgaac tgccactttt
    38341 gaacgatcgc cagcttcacg ggcgatgctg acaatttcaa cgattccatc atagacttct
    38401 ggtacttctt gctcaaataa gcggcgcaat aggtctggat ggctgcggct aacaaatact
    38461 tgcggacctt tagacgtatt ttctactcgg gaaacataaa ctttgattcg atcatgtggt
    38521 tgataaaatt catttggcat ttgatcttgt ttagaaagaa ctgcctcaat tttaccaaga
    38581 ttgacataaa tataacgttt atcctggcgt tcgacgattc cttgcatgat gtctttttca
    38641 taagcactga attcgttata aataatcgtt ctttctgctt cacgtacacg ctgtaaaata
    38701 acttgtttag ccgtttgcgc agcgatccgc ccaaaatctt ttggtgttac ttcaaaacgg
    38761 atcgtatcac cgatctcata tgctggattg attaaaagtg catctttcaa tgagacttcc
    38821 aactgtgaat ccatcacttc ttccgttact tcttttaccg catagacatg gatctttcct
    38881 tttttctgat caaattccac ttcgacgttt tgtgcttggc catagtgacg tttgtatgca
    38941 gaaaccaatg ctgcttctaa agcatcgatc acgatttctt ttgagatccc tttttcagct
    39001 tctaaagcgt ctaacgcatt taacatttct ttactcattt tttctgattc ctctccgtga
    39061 ttaaaattgg atagctaagc gagcttttgc aatatttttt cggtcaaaag tcaattcttt
    39121 ttctcgtgtt tttatgcgta ttttcaatgt aagctgttca gcatcaaatg attgaagaaa
    39181 tccttcatat tgtttttcgc catctactgg ttggtataag gagacatgga cataatcacc
    39241 aagtgccttt tcatagtcgc tttctttttt caatggccgc tctgctcccg gggaggatac
    39301 ctccaggaaa taagcttgag gaatcggatc tggatctgct gcatcaagtt tttcactcag
    39361 tttctcactg acaaaggcgc attcttcgat atcgataccg ccttctttgt caataaatac
    39421 tcgtaagaac cagctttttc cttcttttac aaattcgact tcgacaagtt caaaattttg
    39481 ttcatctaaa atcggtgtca ccatttctgt gactgtttca acgacgctac tcaaaagttt
    39541 cgcctcctta aaaaattaag tgcgtaattc cattaaaaag agtgagcgaa aaatccgctc
    39601 actcacagtt agtatcatta cctataagaa ctataccata ctctgcttaa aattacaagc
    39661 agacaggaaa acagatgctt atagcatatc gaataacgat aattgatttt catctggtag
    39721 atctttcaat acgccattgt cgttcatata ttcgatcaat gtttttgaaa ctttaccgcg
    39781 ggtggcaaga tcttctttgg ataaaaatgg accgtctttt cttgcttcaa cgatttgttt
    39841 tgccacgttg gttcccaaac ttgggacagc tctaaatggt gcgatcaacg tatcaccttc
    39901 aataacaaag ttaacggcat ccgatttata caaatcaatc attccaaatt tgaatcctcg
    39961 ttccagcatt tcattggcta actccagcac tgtcagttga ttcttttctt ttacggaagc
    40021 atctaatcct ttgtccgtga tttctttcat tgcttgtttc acagcatctt ttcctttgca
    40081 catggagact agatcaaagt cgtctgcacg tacagaaaag tatgcacagt aatacaaaat
    40141 agggaaataa accttgaaat aagcgacacg aagagccatc aatacgtaag ctgcagcatg
    40201 ggctttcgga aacatatatt tgatttttga acaagaatcg atataccagt cgggcacatt
    40261 attttctttc atcgccgata gataggtttc tcgcaattca tcagggatct tgttccattg
    40321 tcctttacga acagtttcca tgattttaaa tgccatcccg ctatctaatc cagcatggat
    40381 caaataaacc ataatatcat cacgacagcc gattacttcc gccagtgtcg catctcctcg
    40441 tcgaatcaat tcttccgcat ttcctaacca tacgtctgta ccatgagata gtccggaaat
    40501 ttgcagtagt tctgcaaaag ttgtaggatg cgtttcttct agcatccctc gaacaaaacg
    40561 agtgccgaat tctggaatac ctaatgtccc tgttttggaa taaatttgtt cctgacttac
    40621 tccaagtact tcaggaccgg caaatatccg catcacttcc gggtcatccg tagggatcgt
    40681 ttgtggatcg atccctgata aatcttgaag catccgaata acagttgggt catcatgccc
    40741 tagaatatct aatttcaata cattatcgtg aatcgagtga aaatcaaaat gagtcgtttt
    40801 ccattccgaa ttttgatcgt ctgctggata ttggatcggc gtaaaatcat agacatccat
    40861 ataatcggga ataacgatga tcccccctgg atgctggccg gttgtccgtt tgactccagt
    40921 agcgcctttt gctaatctgt cgacttctgc acttcgaaac tgcagattat tgtccctttc
    40981 gtatcctttt acatagccat atgctgtttt gtcggcgact gtaccaatcg ttccggcacg
    41041 gtaaacatag tcttcgccaa acaatacttt cgtatagtta tgggcttctg cttgataatc
    41101 acctgagaag ttcaaatcaa tatcgggcac tttgtctcca tggaatccta aaaatgtctc
    41161 aaatggaata tcatgaccgt ctttgtttaa gcgtgcacca cattttggac attgtttttc
    41221 tggcatgtca aatccagaac catacgtacc atcttcaaaa aactcggaat attggcactc
    41281 tggacaataa taatgagggg ctaacggatt aacttcagtg attcctgtca tcgtcgcaac
    41341 aaagctagag ccaacagagc cacgtgaacc tactagatag ccatcttcat tacttttatg
    41401 cactagtttt tgggcgatca gataaataac cgagaagcca ttaccattaa tagaattcaa
    41461 ctcttttttt aatcgttttt gaatgatctc aggtaacgga tctccgtaca tttgttttgc
    41521 ttttgtgtaa ctcaattctg atatttcatc ttctgaacct ggaatcttcg gtgtataaag
    41581 ttcatctttg actggaatca cttcttcgca gatatctgcg attttattcg tattttctac
    41641 tacgatctct ttcgctgttt cttcccctaa aaagtgaaat gcagttagca tctcatcggt
    41701 tgttcggaaa tggacatccg gcaaactatg acgattcaac ggattcgctc cgcccataga
    41761 attgatcaaa atttttcggt aaatggcatc ttcttcgttc agataatgta cattacctgt
    41821 tgcaacaacg attttatcca aactttttcc gatttctacc aaattttgga taatttcttc
    41881 caaatcatgt tcatttttta ccaattcttg ctcgatcaaa ggcgcataga ccgcttttgg
    41941 cataacttca atataatcgt agaattttgc tcggtttctt gcctcttcta cccctttttg
    42001 catcatggct tcgaatattt ctcccttatc acaagctgag ccgatcaata gattttcacg
    42061 catctttttc aattgggagc gtggtattcg tgggacgcgt tcgaagtatt cgacattcga
    42121 cattgaaatc agtttgaaca aatctttcag tcctgcctga ttttttgcta aaatcgttac
    42181 atggaaaggt cgagcccttt tgtaagaatc tccttctccg atatgctcat ttagctgatc
    42241 atgataaagc atgttatgat tgtccattgc ctctttcaca aagatccatg ctaagtgacc
    42301 agttgcttca gcatcataga ttgctcggtg atgctgttcc aagctgacac caaatttttt
    42361 agataacaca cctaatccga aacgtttgaa ttgaggatac agatatctag ctaattccaa
    42421 cgtatcaatg actggattgg ccgcttcagg aataccatac cttgcgtagc tagtattcaa
    42481 gaaccccata tcaaaagccg cgttgtgggc taccaaaatt gtatcttttg aaaattcaag
    42541 gaacatccgc aatacttctt cttccgactt tgatcctcga accattccat ccgtgatacc
    42601 agtcagatct accgttgttc gtgacaaagg atgtcctgga tcgataaatt catcaaagct
    42661 ttcgatcaca tttcctttat acattttgac tgcggctagc tcgatgatcg tgtcatatac
    42721 tgcagacaat cctgtcgttt ccacgtcaaa tacgacatat gtggcttcac tcaatgcttc
    42781 atgtgcatca ttataagcaa ttgggacacc atcatctacg acattcgctt ccacgccgta
    42841 taagattttc acacctgctt tttttccagc actatgtgct tccggaaagg cttgcgcacc
    42901 accgtggtct gtaatggcga tagctcggtg tccccatttc cctgcttgtg ccacaagatc
    42961 agagatgctg tttgttgcat ccatcgtact catattgcta tgaacatgaa gctctactcg
    43021 tttttcccct tcaggagcat aatctttgcg aggtgtatgt ttgacttcta tgatatcttg
    43081 cgcattcatg accaaatcac ggacaaacgt atcttcttga atacttcctc gtacctttaa
    43141 ccagctaccg acactgatcg catcaaatac ttgttcatct ttttcgccgt tcgagaattt
    43201 tttgacgata aatgatgaag tataatctgt gattttcaaa gtcaagatct tgcgttttga
    43261 acgtagttct ctgacttctt tgtcgaatac gtacccctca agtgtgactc ttcgttcttc
    43321 ttcaacgata ctgatcattg gtgtcgttgg ttcatcggct ggaatatttc gtcctagctg
    43381 gatgtggatg ggtccttcaa gagctggcga ttgctctttt ctttcctttt tcttttgttc
    43441 gtggacaact agactttcag ctgcttgctt cataaaggct tctgcctgtt cttgtttgcg
    43501 ttcttcaaaa agtttcaata ctcgttccgc ttgctgctca tccacttctg gttcgatacg
    43561 aaattttggg aatccgtaac tgacgaacaa ctcttctact aatggcaagt attgttgctt
    43621 caaataagaa atagctcctg tactgtcgat tggcaagatg actttacgat cttttatcac
    43681 aggtacctgc gttttcaata cttgctgtgc tagtggtgta tcgcattgct gattttccaa
    43741 agcttgggcc cagtaatctt gcaagagctg ttcatcgaat gtctggtcat ctgctaagat
    43801 attcagtttg acttgtgcaa tatctttaaa agcgatagtc agatgctgag tcaatgaccg
    43861 atatagcata ataggcaaaa ttttggaaaa atgcagcgta aattcccata aacgcgattt
    43921 acgatggacc actacactat ccatttcacc agcagtaagc agtggatgat ttttttctgc
    43981 ttcctctagt tggatttgtt ccaacagttt ttcaaataaa tctcgttttt ctgacaaaga
    44041 tatacgccct ttctgatgag aaacaatgca attgatcgca tcttatcctc ttctttttca
    44101 gtacctttta gtatactcgt tttttctgag ttttacatta aaaaattgga actgcgattc
    44161 aaatcgcagt tccaatttta aaagcgaatc atcagattgt caaaaacagc tgattcagct
    44221 atttttattt tctgtgtttt aacgcttatt attcgccttg gttcaagagg attggtaaag
    44281 tattcaccaa ttcttcttta cgtacttcaa ccatttcgcc agttttcttg attttcactt
    44341 ctacgattcc atccactgct tttttaccga cagtgattct gattggacag ccaatcaaat
    44401 cagaatccgc gaatttcact cctgcgcgtt catttctgtc atcaaccagt acttcatagc
    44461 cagcttttgt catcgcttct tctagttcat tcgtcaggtt tgtttggtat tcgtctttta
    44521 gattcatttg gacgatatgc aaatcaaatg gcgcaattcc ttttggccag ttgattcctt
    44581 gttcatctga atgttgttct acgattgctg ataataaacg acttacaccg ataccataac
    44641 agcccataat cacaaatttt tctcgtccat tttcgtctag tacagttgct cccatagatt
    44701 cactgtaacg agtgccgatt ttgaagatat gtccgatttc gattcctcga gtaaaggcaa
    44761 gaacaccatt gttgtctggt gaaggatctc cttcttgtac aaaacgaaga tcttcataag
    44821 aaagtacagt gaaatcgcgt tctggattca catttttata gtggaatcct gtttcgttcg
    44881 ctccagtaat ggcattcgct aaatcttgga cataacggtc tgcatatact tttacgtcat
    44941 ctgaaacacc gactggtccg acagaaccaa actcagcacc tagatatttt tgtgcttctt
    45001 cttctgttgc ttcttctaaa aagtcagcag ataaaaagtt tttcagtttt acgtcattga
    45061 cttcatggtc tccgcgtacc aaaacaagga caggttgttc atccgcaatg aacaatacag
    45121 acttgatgat tttttgaggt tcaacagaaa agaaatctgc tacttcgtca attgatttta
    45181 cgttaggtgt agctactttc tccaattcaa gctgtgtttc atgcgatttt tttgatacat
    45241 agtagcttgt agccatctcc aagtttgcag cataatcact ttcagtcgaa taacagatcg
    45301 tatcctcccc gatttctgag atcgccatga attcttttga gtctttcccg cccattgcgc
    45361 caccgtctcc gataatcgag cgaaattcta agccacaacg cttgaatact tcggtgtagg
    45421 ctttttcgta atctttatat gtctcgtcca aactttcttc cgttgcatgg aaagaatacg
    45481 catctttcat gataaactct cttccgcgaa gcaaaccgaa gcgaggtcgt ttttcgtcac
    45541 gatatttagg ttggatttga taaagattta aaggtaattt cttatatgaa ttgatttcat
    45601 tacgcatcaa atcagtaaaa gtttcttcgt gagttggtcc aaggatcatt tttcgatcat
    45661 tgcggtcttt gaaacgatac aagtttggtc catatgtttc gtaacgcccg gattcttccc
    45721 aaagttctgc tggcaagatg gcaggcatca gcatttctac cgctccgatt ttttcaaatt
    45781 cttcccgcat aatgcgtttc agtttttcca atacacgatt tgctaatggc agatatgaat
    45841 aaatacccgc tgatacttgg cggatatatc cagctcttag caagatttga tggcttaata
    45901 cttctgcatc atttggaact tcgcgtaatg ttggaatcaa catttttgat tgtctcattc
    45961 tatatactcc tttatttcct caaaatagtt atcgtttgtt attataccct atttgtcaac
    46021 tttttctaga aaaagaatcg ttgaatatcg ttccatgtaa ctagcaccat caacaacatt
    46081 aaaaatccaa agccagctaa tgtcaatatt ccttcttttt cttgacttaa tggtttcccg
    46141 cgaatccctt caaaaatatt caagactaat ttcccgccat ctaaggcagg aatcggaagc
    46201 agattgacga ttccgagatt cattgaaaga agcgccatca aactaataac agttgttaca
    46261 ccttgatttg ctgcttctga agacaattgg tacatcatga caggtccgcc taatttatct
    46321 aaactgaaac ctgtaaatag tgatcctaag gctttgaaaa tttccaatga accgctgaaa
    46381 gcttgacgcg tccctccaat gatcttatcc ataaaacctg tattcattgg ggcttttata
    46441 ccaagttggc caactttttc gccatttgac tctacagatt tcggtgtcac gtcgacagat
    46501 tgcacctgac cttctcgttc gatcttaaag tctaatgttt tatccggatt tttcgtaatg
    46561 actgttgtca gatcattcca tgaatggatt tcttttccgt ccacactgac tacctcatca
    46621 ttttctttta agcctgcttc tgccgcagct ccattaggca tgatctctcc tacgcgatta
    46681 gtgttcgtca cttgaacacc accttgcata aatgccagaa tgatgaataa caggattgcc
    46741 aaaataaagt tattcatggg tccagcaaag ttggtcagca tacgctgcca caatttagcc
    46801 gattggaact gtacgtcttt tggcgcaatt ctgatttctg ttccgtcgtt ttcaataatc
    46861 gtcgcatcgt gatcaacagg gtaggttact acttctgttt cgtctccatt cacataacca
    46921 gtgatagtca gctcatcttc taaatcataa cggctcaatt ccatcgggat agcatttgtc
    46981 aattggattt tcttacttaa atttatcttc tctacgatgc cgtcagaatt tagtaaaaga
    47041 gaaagaggca ttcctggtgc catttctgtt tcatcatccc cattgcctgc cattcgaaca
    47101 taaccgccaa taggtaatag gcgaagcgta tatgttgtcc cgtcttttgc ttgatgccca
    47161 taaattttcg gacccatacc gatagcaaat tcgcgaacga ggattcctga tcgtttcgca
    47221 aagaagaaat gaccaaactc atgaacaatc actaatatac caaaaacgat gataaatgtc
    47281 agaatcgttt tcatagcttt tttctccttt acttgttttt tgtgtttttt ataaaacaac
    47341 gataaaacac tataatttta ccatagtata ccaaagacac caatagtatc aatataatga
    47401 aaaataaaaa cctgcgtctt ttatgaaatt cacaaagaaa cgcaggtata cttactaaat
    47461 aatgccaaat aaatgcatga taggaaatac aaacagcaaa ctgtcgaaac ggtctaagat
    47521 tccaccatgg cctggtaaga tatttcccga gtctttcaca tcataatgac gtttgattgc
    47581 agattcgacc aaatcaccaa attgtccgac tgctgaaaaa ataatcgtca tcagaagcat
    47641 gacaaacata ccatgattaa atagttcttt tgctgggtat agcaataaat aaagaaatgc
    47701 gactacaaca gcactaccaa taccaccgag cgcaccttca atcgttttgt tgggcgaaat
    47761 atcaggactt aacttatgct gaccataacg tcgaccaacc atatatgctc cgatatcagt
    47821 tgcccaaaca ataaatagac caaatatcaa aacagccaag ctttctgttc gtgcattgac
    47881 aaaattctgg aaacctattc ccacatacaa gctgacaagc acaggaaaac ctgcttcatc
    47941 aatcgtatac gtatttttag aaataactga tgttcctaaa agaatcataa ctataagata
    48001 aaataacata aatgtactta cattctctgg aagaaagaaa aaccatcttt cttttggcaa
    48061 aacaagtaac actgcaccaa cggcggataa aactccttca aaacttaaga gtgtcaatcc
    48121 tttcatccga aacagctcgt aaactcctat tactgctaag attgctgctg taagctcaac
    48181 cgctatgcct ccgatccata taatcggaat aaacaagact agcgccacgg ctgctgtgac
    48241 cacacgttgt ttcatgactg atcccctttc tcatcttctg ttttttttac accgccgaat
    48301 cgacggtctc gattttgata agaggcgatt gcttcttcaa gatgcgcacc atcaaaatca
    48361 ggccaaagcg cttttgtaaa atacagctca ctatatgcga tctgccataa cagaaaatta
    48421 ctaattcgct cttctccact tgtacggatc atcagctcag gatcttgtaa ctctttaggc
    48481 aagaaacctg tcattaaatg cttagcaatc aattcatcat cgatttcttc tgcgtgtatt
    48541 tctccttttg ctacttcttc ggcaatttct tggacagcag tcacgatctc tgctctgctt
    48601 ccataattca aagcaaaatt caataccatt ccagtattgt tttttgtttg ctcaattgca
    48661 cgtcttacag catcttgcgt atgttctggc aatacgtttt cataacccat gacatgaact
    48721 ttgacatttt ctttaatcaa ttctggtacg aacgtatcaa agaagtcgac aggtagttgc
    48781 atgagaaaac ttacttcatc ttttggtcgc ttccagtttt cagtcgaaaa ggcataaagc
    48841 gtcaacactt tcactccaag ccttgatgct ttttttgtga ctttctttac tgtttccatg
    48901 ccttctttat ggccagcaac tcgtggcaaa cgacggtttt gcgcccaacg accattgccg
    48961 tccatgatga tggcaatgtg ctgaggaatt ggtccatcgg tattaaattg aaattcgctt
    49021 ttttcttgaa tatatttgtt tttttgcgga aaaaaacgta acattctttt tcctcctata
    49081 cttaaaagat tcccactatc ttaagtatta tagcaatatc taactgtttt tcctatgaaa
    49141 tcattgtttt tttatttaaa aactgaataa ttttttcttt ttttaatttt taaataaaag
    49201 aaatataaat aaaaacagag gaagcggaat gatccgcccc tctgttttta tgattttatt
    49261 aaacttccaa taattctttt tctttatcag cagcgatctt atctacttct ttgatgctgt
    49321 tatctgtcaa tgtttgtacg tctttttcta atccgcgcaa atcatcttcg gtaatatcac
    49381 catttttttg ttgtttttta tattcatcga tcgcatcacg acggatgtta cgaatcgcga
    49441 tttttgcatt ttctgcttct tttttaacat ctttagctaa ttcttttctg cgttcttctg
    49501 tcagttgcgg gataactaag cgaatgacat ttccatcatt ggttggactg attccgatat
    49561 cacttgccat aatagctttt tcaacatctt gaagaatgct cttgtcaaaa ggtgtgatca
    49621 ttaaaacacg tgcttctgga atttggattg atgccatttg gttcaatggt gttggcgcac
    49681 cgtagtagtt cacagtaata cggtccaaaa ggctagcgtt cgcacggcct gcgcggattt
    49741 gcccaagttc acgttgaaga ttttgtgctg cttttttcat tttttctttt gcttctgtca
    49801 taattgcatc tgccattttt atttccccct tacagttgtt ccgatatttt cgcctaagat
    49861 tgcacggcga atatttccgt gttcatttaa attgaagaca agcagtggaa tatcattatc
    49921 catgcttagt gaacttgcag ttgaatccat gacttgtaat cctttggcaa taacttccag
    49981 atgagtcagt tcttcaaatt ttactgcatt tgcatctact ttcggatcag cagaataaac
    50041 accgtctaca ttgtttttag ccatcaggat aacatctgcg ccgatttctg ccgcgcgtaa
    50101 tgctgctgtt gtgtctgtcg agaagtacgg gttgccggta ccgcctgcaa agataacgat
    50161 ccgtcctttt tctaaatgac gttctgcttt acggcgaata tatggctctg cgatttgacg
    50221 catttcaatc gaagtttgca cgcgtgtcgg tacacccacg ttttccaacg tatcctgtaa
    50281 agctaaggca ttcataactg tagctaacat acccatgtaa tcagcttgag cacgttccat
    50341 gcccatttgt gctccgattt gtccgcgcca gatatttccg ccgcccacaa cgattgccat
    50401 ttcaacgccc agctcgtgaa cttctttgat ttcttggatg atttctttga ttactggtgg
    50461 tttgattcca aatcctgcgt ccccagctaa agcttctcca ctcaatttta agactacacg
    50521 ctgatatttc ggtgtgacca tgttcattcc tccaatattt tctattgcca ttttaccata
    50581 cttagatgat ttcgacactc taagactggt ttttcttgtt ttgcttaaaa aaagggagcg
    50641 cacaaaccgt gcgttcccgt ctgttattca acagaattat tttttcactt ggctcataac
    50701 ttcttctaca aagttatctt cgcgtttttc gataccttcg ccaacttcga aacgtacgaa
    50761 tgttttaaca gttgcgcctt ttgatgcaac gtatttttca acagtcatat caggatcttt
    50821 aacgaatggt tggtccacca aagcgatttc tgctttgaat ttcttcaagc gtccttctac
    50881 cattttttca acgatattcg ctggtttgcc ttcgttcaat gcttgttctg taagaacagt
    50941 tttttcatgt tctaattcag cttctggaat ttgtgattcg tttacataac gaggattgat
    51001 agctgctacg tgcatagcta cgtctcttgc aactgtttcg tcagttgttc catctagaac
    51061 agtcaatacg gcaatacgtc cgcccatatg caagtatcca ccaaatgcag cattgtcgtc
    51121 tttttctaca acttcaaagc gacggaagct gattttttct ccgataactt gagttgcttc
    51181 gatcaagtca gattcaattg tacctttatc tgttttgatt ttcattgcag cttccatgtc
    51241 tgctggtttg ttttcagcaa caagttctgc aatttctttt actaaatctt ggaacatttc
    51301 gtttttagaa acaaagtctg tttctgagtt tacttcaacg attgctgcag tgttaccttt
    51361 cacagcaact gaagccaatc cttcagctgc aatgcggtca tttttcttcg cagcctttgc
    51421 cattcctttt tcacgtaaaa gatcaactgc tttttccatg tcgccttcta cttcaactaa
    51481 tgcttttttc gcatccatca ttcctacgcc agtcatttcg cgtagttctt ttaccatttt
    51541 agctgtaacg tctgccatgt ttaactcctc ctgtgtgttt ttctttttaa aaaaagctgc
    51601 ttcaaaggag agtgaggctt gtcgcccagc ctttgaaaca gctccatcat tcaaaattat
    51661 tctgctgaag aattgtcgcc ttcaacaaca tctacgattt cttcgattga tgttgctgtg
    51721 ttttcttctg tgaaatcttc ttcaactact tgatcttcac cttggtttcc ttcgatgaaa
    51781 gcatcagcca tttttgaagt aatcaattta acggcacgaa ttgcatcgtc gtttgaaggg
    51841 attactacgt cgatttcatc tggatcgcag tttgtatcaa ccatagctac gattgggata
    51901 tttaatttgt gtgcttcttg aacagcaata cgttctttac gtgggtcaac gatgtacatt
    51961 acatctggga ttcttggcat atcagcgata ccgcctaaga atttttcaag acgttcacgt
    52021 tctttgttca aaccagcaac ttctttttta ggtagaactt caaaagttcc atcttcttcc
    52081 attgcattga tttgtttcaa acggctgata cgtttttgga tagtatccca gtttgtcaat
    52141 gttccgccta accaacgatg gttaacgaag tattgtcctg cacgagttgc ttcttctttg
    52201 attgcttctt gtgcttgttt ttttgtacct acgaataatg ctacgccgcc ttcttcagca
    52261 acgctcttca tgtaatcata agctgcatct actaatttaa ctgttttttg caagtcaatg
    52321 atgtagattc cgtttctttc tgtgaagata tatttcttca tttttggatt ccagcgacga
    52381 gtttggtgac caaagtgtac gccggcttct agtaattgtt tcatagaaat tactgccatg
    52441 tttgtttcct ccgatttggt tttatttttc ctcttcttat cttctacctt gccggcaaac
    52501 tttttcaagc acctaccgac aatccaataa gaatgtggat ttgctgatgc ataaagcacc
    52561 ctcagctatt atacagacaa aagatccgta tcgcaagagc aaatatactt ttctgctcaa
    52621 cattcaatag tttacctgaa agataaaaag aattcaagaa aaacagtaga tttctctttg
    52681 tttctttatt atcctttttc cataaaataa ttttaggtat aaaagacgaa tattattggg
    52741 caatccttct tttttttgtt atgatacatg tgaaatcttt cgtaaaggag aaagaaaatg
    52801 cgcaaagctc aaagacattt gctgattaaa caagtcattg aagaattccc gatccgtacg
    52861 caagaagaac tattaaacaa acttgggcat tatggcgtta ccgccacgca ggcaacgatt
    52921 tctcgagata tccgtgatct aaaaatcgtg aaggcacctg atgaaaatgg cgtttcccgc
    52981 ttcgtattgt ttcgcgaaaa aggaactcct gagactagga ctgaggaaga ggatcggctg
    53041 gttcaaatga ttgaagacat tgtattgaaa gtcgaacgag tccacttttt gacgatcgtc
    53101 aataccttgc cagacaatgc tcatttattt gcatctgttt tagatgagat caaacctccc
    53161 tctattgtca gtacaattgc tggattcgat acgatcatgg tcatctcgga aaatgaggag
    53221 gacgcaaaac gagtagaaga atttcttcac catccaactg aagtatcact tttttaatca
    53281 gagcacgtat tcctaatatg gaatgcgtgt ttttttattt tcaatatttt ccttattgaa
    53341 taaaccttta cactctttac aaaaatacat cattttttat tcaaattttt caacaccttg
    53401 attgaatcac aataatcatc atttatattg gatgttaaca agagcagctt cttgctcttt
    53461 gtgtatttca aaaaagaaat gaaaggaaat catatgtata aattattaac ttctggacca
    53521 ttgacgactt ctgaaacagt gaaaaatgct atgctgatcg atcattgtac ttgggatcaa
    53581 gaatacaaag aaattacaca atggataaga aaaaaattat tggatcttgc tcaagtatcg
    53641 gaaggaatat acacaacggt cttaatgcaa ggtagcagct ccttcggggt agaatcggta
    53701 ttaagttctg tgatcaggcc agaagatact gtactgattt gttcaaacgg cgcatataga
    53761 caacgaatga tcaacatgtg cgaatgtctg aaactgaact ataccgttta tgaggtatcg
    53821 gaacatcaaa tcctccaagc tcatgtgatt acacagctga ttgaagagaa tccgtctatc
    53881 tccgcactag caatggtcca tagcgagact acttccggta ttttgaataa tttggaagag
    53941 attgctgctg tcacacaaga aagagatctt ttgtttatag tggacgcgat gtcaagcttt
    54001 ggtgggatac cgatttgtgt agccgaacta gggattgatt ttttgatttc cagctcaaac
    54061 aaatgtattc aaggtgttcc aggcttttct tttgtacttg ctaataaagc ggaactggaa
    54121 aaacgaaaaa atgttagccg aagtttgtca cttgatctat atgaccagta ccgcgtgatg
    54181 gaaaacgaga atggcaaatg gcgtttcact tcccctaccc atacggtttt ggcttttcaa
    54241 caagcgctga aggaactaga ggcagaagga ggaatcgaaa ctcgatattc acgttatctc
    54301 gccaataatc atgtgctacg acaaaaaatg acagagatgg ggttccagcc tttcattcaa
    54361 gaatatcagg ggccattcat cacctctttt ctttacccta aggactggcg atttgatttt
    54421 aatcattttt atcagtattt aaagcaaaat ggatttgtga tctatcccgg gaaaatcagc
    54481 gatcatgaat gttttcgtat cggaaatatt ggcgagatct atccggaaga tattttggaa
    54541 ctgacagcaa ttatcaaaga ctataaggag aacgttaatg attaaaactg taatttttga
    54601 ttgggcagga acaactgtgg actttggctg tatggctcct gtccacgctt ttagaaatgc
    54661 ttttctagaa aaaggaatcc aactgactga caaagaaatc cgctaaccga tgggaaaatt
    54721 aaaatgggat catatccaac agcttctttc cctcccttct gtcaaaaaac aatggatagc
    54781 tatttatgga caactacctc aggaaaaaga ggtggaggaa ttgtatcaac aatttgaaca
    54841 ttatctgttt aaagatttgg tcaagcattc taccttgaaa cccgacacat tggaaacgat
    54901 cgaacggttg agaaacgctg ggatcaatat tggttccacg aatggattta atgctcctat
    54961 gatgacaatc gtagcagaaa ccgcaaaaaa agaaaggtat tcaccagact tcatggcaac
    55021 ttttgaagac gtcgaagggt atggtcgacc gtttccttat atgatccata aaaacatgca
    55081 gtacttcaaa aacaagtcgg ttgatgaagt aatcaaagtc ggagatacgg tctcagatat
    55141 ccaggaaggg aaaaatgccg gagtgttgac agtaggtgta attgaaggta gttcgctaat
    55201 ggggatttct tatgatgagt accaaaaatt agagatgggg aaaaagatcg tttaaaacag
    55261 tctgttgctc ttagattcca acaagccggc gctgatattt gtgtaaacaa tttaaaagag
    55321 ttaacagaat acattttgta cgctgacatg aatccagaat gacttttata caaaataaga
    55381 ggttgtgaaa aagctgtcct gtatcaagta ataaaaacag ccaagcaaaa atagctcctc
    55441 atatttttcg tttggttagg cttattaccg cagatgcaag cttttgaaca ccgtttattc
    55501 gaaaaaaaaa agggagctgt gacacgactt ttgtcacagt ccccttattt gcaaacaaac
    55561 agtgtttaag agacaacctc tccactaaac ggtgttcatt cagctgactc tcggtatttg
    55621 ctcaaaaaac gataaaattt gaaaagctat tttctctttt gtttctctaa tactcggatc
    55681 agaacgctga tttcacattc tctttttctt acatcttatc tggtgcatga agtcctagca
    55741 aacgaaggtc ttctttcaat aatacagtca ctgcataaac tagagctaag cgtgcttctt
    55801 tttgttcatc atcagctaaa atcttcgtat gagcatagta tttgttgaat gcttgtgcta
    55861 atttgattgc atgtttcgca atcactgacg gttcgtattt ttccccagca gacaagaccg
    55921 tttcagggta tttttgaact aatttaacca cttcccagct ggtatcgtca ttcaatgcat
    55981 agtctgctgt ttctgaaggg acaaatcctg ctttttccaa caggctgact gcacgagcat
    56041 gtgtatattg cacataaggt ccagtttctc cttcaaatcg aacaacttct tccaggttaa
    56101 agtcaaatgt attcagtcga tcgtttttca aatcgtggaa aatgactgcc ccaaccccaa
    56161 cttgtttagc tacagcatct ttgttttcca aatcagggtt tttctcgctg atttgttctt
    56221 tagcagattg gattgcttca tttaatactt cttctaataa gacgatcttt cctttacgag
    56281 ttgataattt tttaccacct tgcgtgatca aaccaaatgg aatatggtgc atgtcgtcag
    56341 accagtcaaa gcccatttcc ttcaagacag ctttcaactg tttaaaatga tagctttgtt
    56401 cattccctac tacatataga gattgtttga agtcgtaagt acgtttacgg tacaatgcag
    56461 cagctaagtc acgtgtaatg tatagtgtcg caccgtctga cttcttaatc aacgctggat
    56521 tcaggtcata agcagatagg tcaacgatct ctgcaccttt atcctcgttc agcaaatgtt
    56581 tttcttctaa aagtttgacg atttcatcca tcttgtcatt gtagaaagct tcaccgttca
    56641 acgaatcgaa acgtacttcc agcagatcat agattttatt gaattctttc atcgattcat
    56701 cacggaacca ttgccataat tgaatagcct ctttatcgcc ttcttccaga cgtttgaacc
    56761 aagcacgagc ttcttcattt aattctggtt ctgtttcagc tacttcatgg aattggacgt
    56821 ataaacgaag caattcattg attggttctg ctttaacagc ttcttctgta ccccattttt
    56881 tgtaagcgac gatcagtttt ccaaactgtg ttccccagtc acctaaatgg ttgatgcgga
    56941 ttggctgata accgattttt tccataatga aaccgatcga attaccgata actgtcgaac
    57001 gcaaatgtcc cattgagatt ggtttggcaa tattaggaga agacatgtcg attggtactg
    57061 ttccttgatt cccgatgttg ctatccccat aatgttcttt ttctttcact actgtttgca
    57121 aaactttttt gctgataagt tctttattca tgaaaaagtt caagtaaggt ccgacaactt
    57181 cgattttttc aaagttggca gaatcgattt tttctgctaa atctgcagcg atttgctgtg
    57241 gcgcttttcg gtaaactttt gctaatgaaa aggctggaaa tgcaacatct ccgtgttcag
    57301 ctgattttgg attttctagt aattgctcga cttgttccag tgttaaatca tctttgacag
    57361 cgtcatagac agcttttgcg acaagatctt tattattcat ctttattcct cctaaagtgt
    57421 tcccttgatt gttaaacaaa aaaaatctct agcgacggca tacttagtat gccatcacta
    57481 gagacgagat ttcccgcggt accactccaa ttgtcctaaa aaaggaccag ctccatctga
    57541 tatcggtaga cgaccgtctg tttaaaacag aattctccaa gtgcgcttcg ttgtatcttc
    57601 ttcattcggc ttccaccact tccgaattcg ctagccaaga atgatacaag tactctcttg
    57661 ttcaacaaat cttagtctta ttaaactttc ttgaattata tcagaaaaaa aaacgaaagg
    57721 ctagtcgttt cataaaaaaa caatccagat ggcctaaagg agaagaaata ccacctggat
    57781 agctataaat catttcgtaa atacgaaaga cttaacaaat taatcagcta cgataatcgt
    57841 tccttcttca gaagctaaaa gattctcgat attttctaaa gaagtgatga ttgccttagc
    57901 gtttggtttt gcttcaacaa atgcaatagc tgcttctact ttcggaagca tgcttcctgg
    57961 agcgaattgc ttttcatcaa tgtattgttt catttctgaa acagtaacag tttctaattt
    58021 cttttgatct ggtttttggt aattgacata cacattgtct actcctgtca atacgatcag
    58081 caaatccgca tcgatgattt cggctaattt ttctgaagca aaatctttat cgattacggc
    58141 ttctcgtcct tctaatccag tagctgtttc tacaacagga atcccaccgc caccaacaga
    58201 gactgtgata actccttgat cgaccagcgt ttcgatgaca cgtgcttctt taatcgagat
    58261 gggtttaggg gaagcgacta cttttctcca tccacgtcca gcatcttctt tgaatgtcac
    58321 agtggagtca gcattcattt gttctttggc ttcttcttcc gtgtagaatg gaccgactgg
    58381 tttacttgga tttttgaatg aaggatcatt ttcatccaca ataacttgag tgactaaaga
    58441 aaccacatct ttatcgatcc ctttttcctt cagtacttcg cccatggcgt tttgtaacca
    58501 gtaaccgata gatccttctg tcattgcaac acatgtatct aaaggcattg ccggtgtttt
    58561 ctcagaatca gcagcttgct gctgaatcaa taggttaccg acttgcggac catttccgtg
    58621 ggagatgatc aattcatctc cttgttcgat aaatttcact aaatactttg ctgtttccat
    58681 taacgcttct tgttgggctt ttgcacttgc atccgtagac aagatggcat tgccccctaa
    58741 agcgacaact acttttcgtt tggccatttc attcgtcctt tcttcagttc taccgaattt
    58801 tctagataac aaaacgagcc agcgttttgt gattttccct caatgctgac tcgttttttc
    58861 gtgagatgaa tgtggttatg cttaaacgcg agggataaac aagttaccta atgttgctgc
    58921 catgatcgct ttgattgagt gcatacgatt ttctgcttgg tcaaattgac gagcatattt
    58981 gctgcggaag acttcatctg ttacttccat ttcagtgatg ccaaagcgtt ctttcatttg
    59041 ttcaccgtaa acagtgtttg tatcatggaa agctggcaag cagtgcaaga agatcaaacg
    59101 atctgtgttg tgtgtttttt ctaccatttc catattgatt tggtaaggtt tcaacaattt
    59161 gatacgttct tcgaatttat cttcttcacc cattgatacc catacatcag aataaaggac
    59221 gtctgctccg tctacacctt tatcaacatc gtcggtaatc atcaattgtg caccggattt
    59281 ttcagcaaag ccttctgcca ttttgacgat ttcttcttca ggttgtaatt cttttggtgc
    59341 aacgatacgc atattcgctc ctaaaattgc acctgtgact aataaagagt tagccatatt
    59401 gtttcgacca tctccgcagt aagcaacagt gataccttct actgttccaa agttttcttg
    59461 gatagttaaa aagtcagcga tcatttgtgt tggatgccat tcatcagtca agccattcca
    59521 aactggaaca ccagagaatt ctgctagttc ctctaccatt ttttgactga atccacggaa
    59581 ttcgattccg tcaaacatac gtcctagtac tttagctgta tcttctgtgg attctttttt
    59641 acctaattgg atatcatttg cacctagata ttcaggatgt gcacctagat cgatcgctgc
    59701 agtagtaaat gcagaacgag tacgtgtaga tgttttttca aataaaagcg caatattttt
    59761 tccttctaga tagtgatgtg ggataccacg ttttttcaag tctttcaagt gttctgaaaa
    59821 atcgatcaaa tattgtaatt cttcttttgt aaagtctttt tctgctaata agcttcttcc
    59881 ttggaatact gattctttca tattaaacat tctcctttag attgttttta tttaagatta
    59941 aagaggaaat cccgacggca gctgacagta agactgccgt caggaaacaa attacttcaa
    60001 gtcttcacgg acaagtggtt ggctcatgca gcgtggacca ccacgacctc gtgacaattc
    60061 acttgaattg atttctaaca ctttgatgcc atagctacga agcaattcgt tcgatacata
    60121 atttcggtta taagtgacta caacacctgg agcgattgcc aatgtgtttg atccatcatt
    60181 ccattgttct cgaggtgcaa cgatctcatc tccattacca gttgggatca gtacaagatc
    60241 atctaatcca agagcagctt tcatcgtttc gtgtaagtta tctgaatgag taatcttgat
    60301 atcatcacca tctggttcga ttgtaaagac atccatctta ccgtctttac tttgaattgc
    60361 tggatggata gtgaatttat cgtgatctac cattgtaaat acggtatcca aatgcatcat
    60421 tgcacggttg ttcggaattt tgattgccaa cactttttca aatcctgaat tttttgcgaa
    60481 taggtggcgt gctaattttt ctaaagcttt cgcattagtt cgttgagaaa tcccaacagc
    60541 tactacttta tcgcttaaga tcaattcgtc tccgccttcg atatgatctg gattttcacg
    60601 atccaaccat acttcgattc ctttatttgc aaatcttgga tgatgtttca aaatatattc
    60661 tgtaaacatc gattcacgac gacgtgcttc aaaagtcatc ttattgactg tcatcccatt
    60721 tcccattgat gcggaagggt cgcgtgtaaa gtacaagttt ggcattgggt ccatgtaaaa
    60781 tggatagtca tcgtctgctg acagatcata tagactgcta gaacgtactt cgatatcttt
    60841 tgtacgaacc ccagccatga ttttatctac catttcttgc gtttccattg atagaaggaa
    60901 ctcatgaaga ccctctctta cagcattcga tgcaatatga gattcatcca acattttatt
    60961 caagaattgt tctttgacat tgcctgcgtc aatcgcttct gccgctaatt tttccaagta
    61021 aagagtctcg acgccttcat tttgcaatgt cttcgcaaag ttgtcatgtt cttcttgagc
    61081 aatcggaaga tatggaatat catcgaacaa cagacgatcc ataatatctg gtgtcaaatt
    61141 ttccacttct tgtccaggcc gtttcagtaa aactgtcttt aatttcccta tttcagagaa
    61201 aacgtgaata ggtttatcca tgttgtgttg cctccttctt tttgattaca tgtataggat
    61261 atcctccttt tgtaatcact ttcataattt tgtctcattc aaaatgagac attcctcaca
    61321 ttaatattcg ataattaaat aattaaacgt ttttctattt ttaagcgtaa tttattcatt
    61381 ttttattctg tatacacttt caaaaaaata tttattcatt gaaaaaattt ttttgaataa
    61441 attaaaaaaa taaattatat tatcgttgtt agcgtttact atatgtatta ttgttgtttt
    61501 ttatacacaa atcattcgct tcaaaacaaa ttattcaata ccattgtata ttttttacat
    61561 aataacaaat tatatttgta aagtattaaa acttgtttct aatgttggta tgttaaaact
    61621 ttacaaattc tttaaaaaaa gtgacaataa atatagtgaa caataaggag aggacttttt
    61681 aatgcgtgtt aataaaatcg attttcactt ttcaaaactc agatatcaac ctgattttga
    61741 acatttctcg gatgaagagt tccaacagat caaggaacat actgttcttc gttcttataa
    61801 aaaaggacag atccttttcg acgatggtga tcccagacaa cggttgtata tcttgaccaa
    61861 aggactcgtt cgtttagaac gttatgatga aagtgcaact tattactact atgactatgt
    61921 gaatacaaat aacctttttc ctatgtcagg aatgtttgga gaagagacct acacttatac
    61981 agctcaagcg atgactgata ttgaaacatt ctaccttcct gtttcctact atgaaaaatt
    62041 agcacgaaaa aatcccgaac agttgatcta tatgatacaa aaaatttcta aagtagttga
    62101 aatacatgaa ttgcgtatcc aaatcggatt gacctctagc gcctttgacc gggtaaaaca
    62161 aaatttattc attttgaaag aagaattggg cgtaccaaca aaagatggtc atatcaccat
    62221 tccttatccg atcaccttaa aagaattagc agtgaatagc ggcacgacac gtgaaactgc
    62281 tggacaagta atcaaacaac tacgtgattc cgggctgtta gattatgaca aaaagcgttt
    62341 tactttctgt aagacagcta ctactccagc tatgagagaa taacgcgaac aaaaataagc
    62401 agtgaccctg ataaatatca gggtcactgc ttattttcct ccctaaaact tctgaaagat
    62461 ccggcagttt tagatcactg gacaagactc aaagattttc tatgtgtgtt tcagttatgt
    62521 aatattcact gtttgcaaaa aatactgtta gattaactag atgaagcgtc ttgtcctcat
    62581 ccgatctaga ttattcgata ttcctcgaca ataaacgatt actcagtgag ggcaatcaga
    62641 ggaacggata atgataaata agctgtaaaa aatagcaaaa taacaataat gattaaaagt
    62701 atgtaaaaaa tagggctttg acaaaaacgt gtcacagccc tatttcgatt aaacggtgtg
    62761 tacctgtcaa ctcttcggtc acaagtcata atgttcataa acatgaggag ctgttttttg
    62821 aaattgttcc ttgtgaatca gagttaaacg acaggcttac gaactcttga ttaaacggcg
    62881 ttcaaaagct tgcatacgcg gtaataagaa cagccaggtc aaaaacatga gaagccgttt
    62941 ttcctgggct gttcttatta cttgatgcag ggcagctttt tcacaacctc tttattcaga
    63001 taaaaccaag cttggatcag cattcaactg atagctagca aaatttcttt tttgcatttc
    63061 cacatgtgct gctgctccaa tcattgcagc attatctcca caaagtcgaa gagggggaat
    63121 cactagttct acctcaggga gtttagctgc caaagcatcc tgcaatcctt cacgcaaccc
    63181 ttgattggcc gctacacctc cagctataat cagttgcttg actggataat tttgacaagc
    63241 tcgaagtgtt ttagtaatca atacatcgat cacacttgct tgaaagctag ctgctaaatc
    63301 atttttgtct agatcttctc cacgctgttg tgcattatgc accaaattga taaatgcact
    63361 ttttaatccg ctaaaactga aatcataatt atcttcatgg atcatcgctc gcggaaaatg
    63421 gtaattatct tttccctgat gagctagctg atcgatttct tttccgcttg ggtaagaaag
    63481 acctaatact cttccaactt tgtcataagc ttctcctgct gcgtcatctc ttgtctcccc
    63541 aatgatttca tatgatccat cttcttgcat atagactaat tctgtatgtc ctccgctaac
    63601 aagaagtgcc atcaatggaa actggaatgg tttaaccaga cgggctgcat agatatgccc
    63661 tgccatatgg tttacaggaa taagcggcaa ttggtgtgcc caagcaaaag ccttagctgc
    63721 agaaatacca atcagcaacg agccgacaag tcctggtcca taagtgacag cgactgccga
    63781 taaatcttct gccgacacac cagcttctac cagtgcatct tccaagcata aggtgatttg
    63841 ttctacgtga tggcggctag ctacttcagg tactacgcca ccaaaacgtt tatgactgtt
    63901 tatttgagaa gcaacgatgt tagataagat ctctgttcca tttctaacga ccgctacact
    63961 cgtttcatca caacttgatt caatcgccag aatcaattct tcattcattt catcgtctcc
    64021 gtttttaact ttctgctcat aatgatcgca tcttccgtcg gattgcgata atatgatttc
    64081 cgtttaccta atatacgaaa cttttctgat tcataaagtt ttcttgctgc ttcattagat
    64141 agacgtactt ctaaaaaaac atgcagcgaa tcttttattt gttccgtaat cagtaattgt
    64201 aacaaatgcc gtgcatgccc ttttccttgt tctgaaactg caacagcaat atttgtgatc
    64261 tcgacttcat ctattacttt cgagtatcca ataaagccac agatctggtt atcttcagct
    64321 aaaagcagat aatcagagtg aggctgttgg atgtctgcca aaaactgcat tttcgtccaa
    64381 ggtgaaccat gtggataagc attttcgcta atcatccata cgcattctgc caagtacgct
    64441 tcatcaaaat ctttttttct gcaaatcaac taatctcact cattttctaa aagttttatc
    64501 atatccaacg catcttcttt tgtttcagtg taatatcttt ttttgatcgt tgttgcttga
    64561 aaccccaatt tccgataaag tctttgtgca tcttgattac tcatacgcac ttctaatgac
    64621 atcgtctcac agcgattcat tatagcaaag ttctcgattt cgtcaataag cagcgaaccg
    64681 attcgtttac gctgatatgc cggtcgaacg gcaatattcg tgatatggca atctaaaccg
    64741 aacgctcttg aaccgataaa accaatcatt cttccattat caaagatacc tagatataga
    64801 tgaggaagcg gtgacttcaa ttctgatata aaggcacttt ttgtccatgg caattcgcca
    64861 ttatagactt ctcgttcgac atccagcagc tctttgatat cttctgtagt caattctctt
    64921 agtacgtaac gacgatcgat ttgttcgact gttttttctg tataaatcat tttatcttga
    64981 aaaaagcttc tggcatacgc tctaaatttt ttcaacataa ctttcgtctc caggtgtgtg
    65041 cgtctccaac catttttctt ctgcttctac tcgttttaaa tagcgaggaa gaaaagcttg
    65101 tttctctgca actggttcaa ctaaagcacc taattgagcc acagttacac cacttggaat
    65161 atcccatgct ggaactgtat taatcatcgc actaggcaat atttccttta tttcagagaa
    65221 gaatttgtga caatcactcc cgacgaaata aatagcttca ttcagctttt gtagttgtgt
    65281 gaacaaatca gaagcagaaa tatgtacatc ttcaagtaca gtttctaatc tattttcttt
    65341 ccagcggtat gccccagcgt atacattttt tcttcgagca tcaaacatgg gcacaataat
    65401 attatccaca ccgatgcaat tagccgcgat ggttttcaga ctagatactc ctactagctc
    65461 tttatctaat gtataagcaa tcgtttttgc tgtcgttaca cctatacgaa gccctgtata
    65521 agaaccggga ccatcagaaa ccgcgattcg gtcaatgtct tttggactta tttgaagatc
    65581 tgcaaacaat tggtcgattg caggcatcag tgttacactg tggttgcgtt tgatatttgt
    65641 ttgtatttgt ccaagaattt gttcgtcttc taccacaccg atcgccaaag tttggtttgc
    65701 agtatctatt cctaatgtaa tcatatattt gatcctttct gctatgacat tctcgccttc
    65761 cattgtagca tactttttta ttctgaggat gaaaatacca aaggcaaaac tagtacaata
    65821 atacatgatt atctgctggc gggcaaattt tgccccttcc tgaaacacaa aaaagctgcg
    65881 gtatcactcc attttcccac agcttttcca tcttttattt cgttgattct cgaataatca
    65941 actctgtttc cacaataatc tttcttgaaa ccggtgcttt ggaattactt aattgaagaa
    66001 tcgtctccac tgctagctct cccatccatt cggtataaac ttttacagta gaaagcgagg
    66061 gcgttacata tttcgctaca ctgacatcat tgaagcccat cacagaaata tcttctggta
    66121 ccactaaacc tttttcttga atcgctttca gtgcgccaat cgctaatgca tcattagctg
    66181 caaaaagcgc atcaggaaca tgcggattcg tttttaaata ttgcttcact gcttgatatc
    66241 ctgactcgac agaaaaatct gcttcgataa ttggaccttc gtcgtagcct ttttctgcca
    66301 aaacttgttg aaatgccata attctgggat cttttagccg ctgatggttt ttcttcgtat
    66361 attctgttcc agcaagcata gcgatatgtt gcgctttatg ggagacaaag tgttcaatga
    66421 ctttttgaac acttgactta aagtcgacaa ctagagagtc gattccttga gaagtgccgt
    66481 cagaatctac caaaagcaag gcattttggg atcctttcat ttctaataac tgagcttcgt
    66541 cgaattttcc tagtgcgatc gttccatctg ccggaagatc actaatggaa tcccacgttt
    66601 ctttcaataa agtaacgttt gcttcttctg cctttttttc gattcctagg cgaatagata
    66661 aataataaag atcttcaagc tcttccgtat cgtcgtacca ctggatcagg cgaagcgttt
    66721 gcttagtatt tgctgcattt ttacgatgtt tcgtataatt taagcgttct gctgcttcaa
    66781 aaattttttg ctttgtggct aagccaacgg aaagatcagg atcatagttg agaactcttg
    66841 aaacagtagc aggagaaact cctgccaact gcgcaatgtc tttaattgtt gccatttgtc
    66901 tctcctcctt ttttacgttt ataaaatcgc agtaaatcgt tcaaacgctg cctgtccttt
    66961 ttcatctcgc ttgaatactc cagcgtcttc cagtacacgt gcaaaaacct gacctacaga
    67021 tttttgtacg acatcggtga ccgtatcttt cgtcaaatca ggatgttctt tttttaattg
    67081 ttctgcccac ggacgatgat attcagccat ctggttttct tctcctaata agaaatgctc
    67141 tacttctact agttctggtt tcaaacgagg tggtaaaaca gcaagcccca ttacttcgat
    67201 caacccaatg ttctcctttt tgatatgttg gacatcttta tgtggatgaa aaatcccatc
    67261 aggatgttct tctgaaacat tgttgtctcg aaggacaagg tccaactcaa agtaattgtc
    67321 ccttcttcgt gcaattggcg taatcgtatg gtgtggtgta ccatctgctg aaaaagctct
    67381 gatagatact gtttcatccg aatatgcttt ccatttttcc aatactgagt tagctgcttc
    67441 aataagcaag tttttctctg ttgattgcaa acggatcaca gacatcggcc atttcacgac
    67501 tcctgcgatg acttgcggat aatcttctag tttgataagt ttttctatcg gtgcttttgc
    67561 cataggaaat tcatgacgtc ctgcctgata atgatcatga gaaagaatcg atcctccaac
    67621 aattggtaag tcagcattcg aaccaacaaa gtaatgaggc aagacttcag taatccgaag
    67681 cagtctagca aatgtttcct ttgtgatttt catcgggcgg tgttgttctg ataaaatgat
    67741 cgaatgttca ttgtaataag cataagggga atattggaat ccccagcttt caccgtctag
    67801 attcattcga ataatccggt gattggtgcg ggctggatag ttcaagcgac ctctatatcc
    67861 ttcgttttcc atgcaaagca tgcaggtagg atagtctact tttaccgcct cgcgttctgc
    67921 tgcgatttgt tttggatctt tttctggctt agataaattg attgtgattt ccagttcacc
    67981 atactccgac tctgccggaa atttgatgtt ttttgcgatg gctcttgttt tgatgtagtc
    68041 atttttctta cttaaattaa agaaatactc agttgcttcc tctgggcttt tcgaatagtg
    68101 ctgtgcaaag aatgcattga cgactgaagg tggcggtgtg agaaaatcca ttaattgcgc
    68161 ttcaaaaatc tcttgttctg ctggcaattc tgaaagaaca ccattttctc ttgccttagc
    68221 gacaagctga tccaataagt ctacagattc ttccttaact ggtcttactt caatgctttc
    68281 tagagcatct tctcctacca tccctaaaag acgattctgt aaatataagc ggtccatttc
    68341 catccatccg ccagcttcga tcgcaagtgt ggcaaaatca caaatcgttt gactgattga
    68401 catatctcat tcccctattc ttcataacca tttggatggc tgacatgcca atcccatgct
    68461 gttttgataa tcgctttgat atctgtgtat tcaggtttcc agcccagaat ctctctcgct
    68521 ttttcacttg aagcgactaa tcggctagga tctccagctc ttcttggtgc tatttctgct
    68581 ggaatttctt ttcctgtcac ttctctagct gcttctagca tttccttgac tgagtatcct
    68641 ttgctgctgc ctaaattgaa gaagttgctt tcatttcctt ctttcaagta ttccaaagct
    68701 aaaatatgtg cagcaatcaa atcttctact tgaacataat ctctgataca tgttccatct
    68761 ggtgtatcat aatcgtctcc ataaacagcc aaggcttttc tttgaccaag agctacttgt
    68821 aaaatgattg ggacaagatg cgtttctggt gtatgatctt ctccgataga tgcatctgct
    68881 tttgctccgg ctacattgaa gtagcgcaaa gcgacatagc gcatgccata tgcttggtca
    68941 caccacttca tcattttttc catcatcaat ttactttcac catacggatt tttaggatta
    69001 gctggtgtat cttctgtgat tggagactct ttcggctctc cataagttgc tgcagtagaa
    69061 gagaatacga ttttattcac gttgaactcg tgcatgactt caagtaagat ttgcataccg
    69121 tatacgttgt tgttgaaata catcaatggt ttttcgacag attctcctac taaagagctt
    69181 gcagcaaaat ggattactcc ttcaatcggt tccttttcaa atacggaacg tagaaattct
    69241 ttatcccgga tgtctccttc ataaaaatga gcatctggat gtactgcttg tttgtgacct
    69301 gtgagcaaat tatctaccac agctacttga tacccctttt gaactagctg gtctacagcg
    69361 tgcgaaccga tataaccggc tccgcctagt actaaaattg tcataaaaaa cactcctttg
    69421 attagtaaaa ttgtgtttgt tgattctata taatttaaaa aacgaagttt acttttatgg
    69481 tgaaaacaaa caaagtaaaa tttcgtaaat tcatatggta aatttataat aaaacattta
    69541 gtaaacattt tcaagggttt tttgcaaagg tttacaaaca aacgtaaaac tagccttttt
    69601 ttctcttttg ttccataata gatataacaa ataataaatg aggtggattc aatgaaaaaa
    69661 tttgtatctg gaatattagt aggaagtctg gcaacagccg ctgccgttgc tggtttggtt
    69721 gcttctgtga aaaaaacagt gatcgatcca attgacgaaa aagaagcaat gatcgaagaa
    69781 aaccgtaaaa aagcaatgcg taaacgcgtt tctcgttaat aattatttaa aaatacgcat
    69841 gaagactttc taaaaaacag ataagtcatc atgcgtattt ttatttgtca gtcaatataa
    69901 gttgtgctat tttatctgcc tgtctcagat tccatggatc tgtataaggt ctagctactg
    69961 ctgcagttag ccaatccaca ccttcaacag gtattccata gcaataaaag tcagactgga
    70021 tttcccctga agcatcaatc acgcgattcg tacctcgctc aacatctata gcttctgaat
    70081 gataacttcc tgtattatct gaaaatgaaa aagggcgaac aagtttgttt tccttcaaat
    70141 tttttatgac aggattactt gaacgagaaa agcttgtttg tggcaatcga gcttcaatca
    70201 aatagcgact gctgatttct ttatcttgta gagaaataac atagcggttg tgcttttgct
    70261 gaatatcaaa tggcggttcg atcaaatgaa aaatacccgc atctatcagt gctgctaatt
    70321 ctttcgtccg aatgattggc ggaccgatcg ttaaaaagct atttagatga gtaaaccatc
    70381 cccatagtat ttctttgtat tccttagcag aaaaaacgtg ccattcaata gcctgccgga
    70441 ttggatcacg ccagtctttt aatgcatcaa atgttgaagc aaatgctcct gtgcaattgc
    70501 ctttttccgc ttcttttatc tgcccttcaa taaatattcg gctagcttct gaaaagggct
    70561 cattctgata ggcaagagga gtttctaaat acgaccaatc ccatctataa gggattagtg
    70621 ctggatgctt tccgagacac tcctctgaag agagggagag aaaatcatgc tcaaaatttt
    70681 tctttggaat cggcaagtga tattcttcga tgattttctt gtaataaaac aactcaacgt
    70741 cttttttcag atagtcaaaa aatgtttcgc cagtcaaaaa tcccgactga tgccattgct
    70801 ccaggttttc ttttgttagt aaccgtggcc atgccattgc tccgccttgc ttttgatttc
    70861 ttcctcgagg gtaataaggg agcccccttc ttgatccact gtatacaatc ggttccttgc
    70921 ctgatggatg atagaccagt tttccctttt cgttctcgaa gcgccctcct cttgatactg
    70981 taaacaatcc gacataatca aaaaaactta agcctagacc tcttaaaaag acattttctt
    71041 taggcggtat cttcgaaaga tctacatctg caggattaga aggaagctga tacagtaaaa
    71101 catttttctt tgcgtagtct acccacactc tttcttcttc tgtctcatca ttttcccagt
    71161 gaccgcttgc taaaacaagc tgatcgatag aatagctttg ctgatctgtc tgtaaaagat
    71221 aaccatcttt tcttttgctt acttttttta ctaaagaata aacaaactga atagaagctg
    71281 gaaattcttc ttgcaacttt gagaaaaacc agcgttgata gagtccgtat aaacatcgag
    71341 tagattgctg atctttttcc agacgattga tttcttctat aattagttgc ttatttttgg
    71401 ttaccgtctg ttcgatataa tctgtagcag gtcctttact ccattgaaac aagttgggtc
    71461 cctgtgttac caatcctcca ctggacaatg tttcatctgt aaaaagcgtc acttgttgag
    71521 aaacagaatt cattaataac tccggtggct gattcaaccg ccaaactcga cctccaggac
    71581 cattgggatc gaaaagaaaa agttcaagag aatgtctgtc ctgattatta tggagaatcc
    71641 tttctgctac acttaatcct ctaggtccgg caccaatgat ccctattttc atttgcatgc
    71701 ctccatttcg ataaattttt tcaataaaac agcatggttc tgatgttcat tttttgcacc
    71761 ataaacaaaa gtaatgactg gatgttcttt cataagttga ctgagtttaa aaatagcttg
    71821 cttcttttct tcttcgaaaa tcaattcttg ctggtatttc tttgcaaatg aagcaaatcg
    71881 ttctggtaca tggtcaaaag ttttgcgtgc ttcattacta ggtgcaattt ctttcaacca
    71941 cagatctaac tgcgcttttt cttttgaaat tcctcgtggc cagaatctat ccacgagtat
    72001 tctatagcca tcctcttttt cagcatcaag gtaaacacgt tttaatttga tcatagttgt
    72061 ttccttcttt ctaccttttc gcttagctta tatatgtaga taatagcaca aaataaaaaa
    72121 aatgaacggc acaactatcc attgcgccgt tcatgtgatc ttggtcaaaa tctaatgatt
    72181 gattcggttt ttaaagctgt catacaatca tctgttttat acttttttac ttttgatttt
    72241 tccagaccat ccgtcatatc cacccttaag taaataaatc tcttgataac ctgctttacg
    72301 taatttattt gctgtacgga tgctcaaact tttcttttga tcgtaaatat agacaggctg
    72361 gtcttttcga atagatccta atgaatcttt caaaacagtg taagggatgt ttcttgcacc
    72421 taaaatatgc ccagcatcaa acacatcttt ttcgcgaaca tcgattactt gtgcttttct
    72481 cattccttct cgaaattctt cttctgtcaa agtcgttgct gaacgtttgg ccattactcg
    72541 tagatagact tcccaaagga tgattgcaag cacaatcaat agcaaaatga tattaattac
    72601 ccataaaacc atgtaatgcc tccgtttttt taagcaaatt tcaaggctaa tgaggcagca
    72661 ccaatgacac cagcttcatt accaagctct gctaatttga tttttgttga ttctcttact
    72721 tgcgggaaag taaattcttt aaaatatttc tctacacggc tacgtaaaaa ttctcctgct
    72781 gccgatactc ctccacctaa cacgatactt gacggattta atgtattccc caagtttccg
    72841 caagctaaac ctaagtagaa gcatacgcga tcaacaacca tcaatgcaaa cggatcgtct
    72901 tcttgagcca tttcaaagat atctttactt gttacttctt ctccgttgtc tagcattgct
    72961 ttcagttttg aatcccctgc gtattcttct gccaagaaac gacctaggcg taccacacct
    73021 gtagcgctag atacagtttc taaacatccg cgtttaccgc aagtacattc gaacccttca
    73081 ggatccacag tgatatgtcc gatttctcct ccagctccgc tcacgccgtg aatcaaattt
    73141 ccgccagcaa cgatacctcc gccaacacct gttcctagag taacgaatac aacgtctgga
    73201 tcgttttctc cagcaccttt ccatcgttca cctaaagcag caacattcgc gtcattatcg
    73261 atagcgaatt tgattcctgt tcctgcctcg atcagttttt tcacaaattg aacttctgtc
    73321 cagtttaagt tatacgcacc aataaccgtt ccagcttcgc tgtccacgct tcctggggta
    73381 cccatgccga ttccgataaa gtcttcggct ttcatattat ataaattcaa acgatgattg
    73441 attgattcaa tgatttcagg aacgatatgc ttaccatcat ccaaaatatt ggtatcgatg
    73501 ctccattttt gctgaatctc tccttctggt gttaaaattg caaattttgc tgttgttcca
    73561 cctaaatcaa tcccaataat cttcttgtcc attacaaatt tcctttcttt cggctttctt
    73621 caattcggtg ctctcttttt aatatgcgcc acgctgtcat gtatgtatca tgatcaatca
    73681 aacggctatc atacagatta cgcaattcga tagtcattaa ttcaatgtca tagatgcgtg
    73741 cacccacata gataaagatg ccaaattttt tgaacagctg ctgtacatca tataagctct
    73801 ccatctttaa gcacttcctt catattatac aagtaaacca tattttttta aacccagtaa
    73861 aatacatatt acaaaaagaa atacaaaaac tagtgtagaa agaatccttt ggtgtatcga
    73921 atagcttgtc tgttgtttcg gtagacccaa aacattccct aacactaatc cgccaattag
    73981 cccaccgaca tgtccccaaa catctacaga ggtatccaga agaccaaaaa caaatgtcaa
    74041 cacaataaat aaaagaaaat gtctgactag ctgctggatc gctgggttat gtttaaaatg
    74101 aaaccctaag ataaacaacg caccgaacaa accgaatatc gaagtacttg caccaccaga
    74161 cagaacattc gcttcgttaa aagcaaagct tgctgtattt cccattatcc cgctgaacat
    74221 ataaatcaag aaaaaacgcc aatggccata gattgcctcg atttgctgcc ccataaaata
    74281 cagtatcagt gagttcatta cgaaatgcat cagtccaaaa tggatgaaga ttggtgtcac
    74341 taaacgccac cattcattga agtgaacaag ataaggacca tacattccgg cataataagg
    74401 gatctcaagt cgcggaaata attccatcat gaggaagacc gctgtctgta tccctaaaaa
    74461 agcataggtc cataacggtc gtcttaacca tgcacgcaat tttatttgct gttgataatt
    74521 catgaaaacc tcccatttca ttttagtcaa taaacaattg ttggatcttc tgatcaaagc
    74581 tctccggttg ccattgttca ttcaattgtt cacgaaaaac aaggctacat gtttttttat
    74641 gaaaattatt taaataccgg tcgtaataac caccgccaaa accgattcga taaccggctc
    74701 tattgtagat aatacctggt acaatgagga gatcgatcat ctccggcaaa tagagcttct
    74761 ctgattgtgg ctcaagaaca ccaaaactcg aacgtacaag aacggactgt tcatccattt
    74821 cataaaacgc catctgacgt ttcggcaaag ttttaggtac aactattttc ttttgttctt
    74881 tttctgctcg ttggataacc ggctgtgtat ccagttcaaa ttcattcgat aaagtcgtcc
    74941 ctatcacttt tgctgacttc cagtactcgc tggcgaatag ctgtttatag attcgctgtt
    75001 ctttttcctt tttcttgcca cttttggcca gatttttcag cttttcgatt cctttccggc
    75061 gtaatcttac tttcaattgg tccaaaaact cacccttcct tctctcataa aatggtatag
    75121 gaaaaaaaga aaaatagcaa tcgaactttc aaataaatgc ataaatagaa taaaaagaag
    75181 acctagaaat gattggcctt ccttttttaa tgaatttcaa ctgttcagcg cgtgtttttg
    75241 tctgataaaa agtgcataca gaaaatatgg cgcaccaaaa agggcaatca aaattcctgt
    75301 aggtacttcc ccacttggta gaatcactct agcaaccata tctgcactaa gcagaaaaat
    75361 ggaaccgaac aaaccgctca gaacaaaggt ccagttattt tctttacgta cgactagctt
    75421 cgcagcatga ggagctagta gtcctacaaa agataaacta cctgtcaccg ctacactacc
    75481 gcttgccaaa gcaacagcta acaacaaaag gattttttga cttttagcca cgttaaggcc
    75541 taatccttgc gcgcgttctt gaccaaatga tagcaataca atcctctgtg cataaagtcc
    75601 tcctagcgga attaagataa ccgcccatgg aagcaatgcc caaacataag gccagctcgc
    75661 accccagatt gatcccgcta accatgcatt cacaaatcca taattttcct tagataattg
    75721 gatcgtgcat agcaatgtta atgcagaaag gccggcatta acggcaatac cggacaataa
    75781 gattcgattc atacccaaaa agccatttcg atcataggcc gcaagataga cacagcaagc
    75841 agctaaaaca ccacctagaa aggctacaac tagcaataga aacggcagcg aatagtgcga
    75901 aaagaaacct aaataaagca tcactaaaaa tcctgctccg gcattaatgc ccaatatact
    75961 agaatctgcc aattcatttc ttgttactcc ttgcaagata taaccagaaa cgccaagaca
    76021 agctcctgct aataaactga tcagtaatcg aggaactcga aattctctaa caatcagcgc
    76081 caaagaagat gattctttcc ctatgaacgt ggcaaaaagc tgatccaggt aaataaaagg
    76141 cgtaccaatc attaaactac ctatagacag gatgacaagt acaaaaagca agaaatagaa
    76201 cgtccaatat atttttttca ccgttgttcc ctccttactt gtatcagtaa aaacggaacg
    76261 ccaatcaatg caatgactaa tccaaaaggt gtttcaaaag ggggattgat catcctcgct
    76321 gtaaaatccg ccaacataac caatccaccg ccaactaaaa aagagacagg tagaacataa
    76381 cgatagtccg ttccaaccat tccgcgaaca actggcggaa ccaacagccc aataaatgaa
    76441 acaggtccga ctaacgaaac ggcaatggct gctaacactg caactagaca gaaaacaatc
    76501 aaccgcgttc gtttaggatg acttccaaga gctaatgcat gtgtttcact caaatgaatc
    76561 aaagatattg cttttcctaa tgaaaaagaa accaaaacag caagtatata taaaggaaac
    76621 aatactgcta attgtttcca tgtgatattg gcagctccgc ctacaaacca aaaagcaaga
    76681 tcctgctgca gattaaagaa ttgggtaagt gcttggctga tagcagtaaa aaaagaacta
    76741 atagctacgc cagctaaaat cagctgtaaa ggattcatgc ccattttcga aaactgcaaa
    76801 aaaccaaata cgagcaaagt tacagcaaaa gaacctgcca atgacattag aaacacagaa
    76861 aaatcagaag ctttaggcca aaaagcaaaa ctaagggcca ttgccaacga agcacccgcg
    76921 ttgattccta gtaagcctga atctgctaaa ggatttcttg tcaccccttg catcaatgca
    76981 ccgctcaatg caaaacaact ccctacaaga aatgctcccg ccacacgagg caaacgaata
    77041 tttcgtatga tttggtgact ttgttttgat ctatcaaaat caaacaacgc ctgccaaaaa
    77101 tctgcttttt ccacagtcgc tgcgccattt gaaagagcaa caaaaaaaat aatcaaaaga
    77161 acaaaacaga ccaaaagcag gagtctattt ttcttcatag tcctcataat tgatcctcct
    77221 gctcttttgg caacaaatca taggttagaa aaactggttt atttgttctt ggaagcgtga
    77281 caatcgttgc atcaatctca aatatttttt tcatacactc ttttgtgatc acttcttcag
    77341 gtgtaccttc tgtgatcaac ttaccgtttt tcattgctaa taaataatct gaaaaacgtg
    77401 aagcatgatt aatgtcatga atcgacatca agatcgttgt ttgtttcatt tgattgattt
    77461 cagtcaataa ctgtaggatc tctaattggt gtgccggatc taaaaatgtt gtcggttcat
    77521 ccaagatcaa gatatctgtt tcctgagcaa gcgccatagc gatccagaca cgctgggttt
    77581 gtccaccaga cagcgttgcg atatcccgca tacgaaatcc agctagacct gtagcatcca
    77641 acgcccactc aattgcttca tgatcttgcg tgtccatgcc actgaacagt ttttgataag
    77701 ggtatcgtcc gtaagatact acttcttcta ctgtcatacc tgtagggtgt tcactagttt
    77761 gtgctaataa tgcaattttt tttgcaagtt cttttgaact gtatgtagag atatcagagt
    77821 tattgacaat gatctttcct gatttcacag gtagtatacg ggtaaaactt ttcaataaag
    77881 tagattttcc acttccattt ggcccaatca aactagtgat tttcccttca ggaatcgtaa
    77941 cagacatgtt ttcaatgatt tttttgtttt cgtagccggc tgcaatgtca gctgctctta
    78001 atctcgtcat ttcacccatc ctttcgctga aaatcattct caattatgac gctgtttcaa
    78061 catttcgtca agcgctactt tcatatctat agccaaatga agcggttgac agcgttcgaa
    78121 atatcaggaa taataaccaa tgagaaagat tctcaattat gttaggagga aagaaatgaa
    78181 attaaaaaca aaagtattca cattatctag catattgctg ctggctggag tactgagcgc
    78241 ttgctccccc tcttcagata aagaaacagc tacaactgcc aattcgacga cttcatccaa
    78301 tagtactcat acagtcacag atacacttgg acataaagta gagattccaa acgcgcccaa
    78361 acggatcatc ggcagttact tagaagacta tctgattgca ctaggggaaa aacctatcgc
    78421 ccagtggact gttggcagtg gttccattca agattatctg caagatgatt taaaggatgt
    78481 cccaacgatt tcttatgacc ttccttatga gaaagtgtta agctttgagc ctgatttgct
    78541 gttgatttct tcttccgcta ctgtcgaagg cgggaaatac gaacaatata gcaaaatcgc
    78601 gccaacttat gttgtaaaaa acggaacaga tgtcacatgg gaagaacaac taaaagatgt
    78661 cggcaaagct ttggcaaaag aaaaagaggc agaaaaaatc atttccgatt atcaaaaatc
    78721 catcaagaaa actcgagaag aactagcaga taaaatcgag aacaagacag ctgcagtgtt
    78781 gtgggtgacc aacaactcag cctttatggt cagcaaaaat cggtcaagtg gaagaatcgt
    78841 atacgacgat ctggaatttg gtgttcctga tctagtggaa gaggtctcca aaaaagccac
    78901 ttctgactgg tctgctgttt cctctgaaaa gctagctgaa ttagatgctg actacatcat
    78961 ccttgtgaac agtgacaaag atgctgccat gttcaatgaa ccaagctgga agaatctaaa
    79021 agcagtgaca gacaatcatg tactgcaatt cggacctgaa tctagttggc tatataacgg
    79081 acctattgcc agtcaggaaa tggtcaatga tatcaaagaa aaactaaatt gattttaact
    79141 aaaaaaagca tcttcacgat tagtcgtgag atgctttttt tcttattaac aaactagacg
    79201 catagatttt attcttcttt tgaagcaacc ctatttttta ctctttccca tttttgaaaa
    79261 cgatgggcat atagattttc ttgatccgtt atcccctcat actcctctat aagggcgaag
    79321 tctgaaaaat caatattccc aatatacgta tcgccttcaa acgtgctatc gatcaatgtt
    79381 cgccagatcc gaccagtgta aggaagaagt tcttgaaaaa tccgactacc acccgaaaca
    79441 tagatatctt ctgagatcgt ttgactatac gacaaaagtt catcgatcga atgcattact
    79501 tccgcatttt ctttcgtgcg aaaatttggt tgcgttgtca acacgatgat atggcgataa
    79561 ggaagtgaaa gatcccccat tccttcatag gtctttcttc ccatcactaa aattcgatcc
    79621 attgtatgtt cgcgaaagaa acgcatatca tttggcaatc gccatggtaa caaaccattt
    79681 ttgccgatca gaccattttt atcttgtgcc cacatggaaa taaacatatt atccgctcct
    79741 tatgatttta gatgttttga gataaaaaaa gtctcaattc tttgaccagc aaggaattga
    79801 gaactttaaa aacattattt tgtttcacgg tgcaaagtaa ctttgcgttc gcgtgggcaa
    79861 tatttttgtt tttccaaacg atcaggattg ttacgtttgt ttttgcttgt tagatagtta
    79921 cgttctttac aagaagtaca ctctaaagtg atgtttacgc gcatgttttt ccctcccatt
    79981 tttacaagaa tcctctaaga tgactctcag acttgattat cttaccatga ttttttctag
    80041 attgctagtg tttttcacga ttttgttaag taaaactact tgacgataaa tacaacggtt
    80101 ttacttctca aacaaaatct ttttcttttt gtataaatac ccttacactt gtttatatag
    80161 attatgtttt ttaataaagg gggatactaa tatgccatgg aacatgaagg actttcctgc
    80221 atcaatgaaa aacttagata aattgacaag aaaaaaagca atcgacatcg caaatgcatt
    80281 attagatgaa gggtaccctg ataaccgtgc gatcccaatt gccattgatc aagcaaaaaa
    80341 atggtatgaa gatgctagtg aatcagaaaa acgaactttc gaaaaagaaa aaaatccaag
    80401 caagacggat gaacacgata cgaatcctcg tgctggaaaa ctgcttgatt cagatgtgat
    80461 cgtagaatat gaggaagaac aatggatcgt gaaatctaaa ggggcaaaaa aggcaagcaa
    80521 ccattttgat acaaaaaaag aagcaatcga aaaaggaaag caagtagcac aaaacaaaga
    80581 atcaactttg ttcatttata aaaaagatgg gacaaaagaa aaagaaatcg attattgaac
    80641 atatgtaaag gggttgtgac gaaaatcgtg tcacaacccc cttatgttcg aaacaatggt
    80701 gttcctctgc caactccgaa ccataagtca taatatctat aaaacgggaa gagctgtttt
    80761 ttgaaattgt ttcttatcac tcagtgttaa acggtaggag aaaacctctt ttttattgat
    80821 atttgatttt catcaaacag tcacatagtc gattgcttct gcttgcggaa tcgtttcatc
    80881 tacaaagtag actttatacc atgtcaagaa actgaagatt gtccagattt ttctttgctc
    80941 ttctttacgt ccttcgtgat ggtcatctag caacttcatg attttttctt gatcaaaaaa
    81001 ttcttttgca aaatctgctt cgaataatgc tttgacttgt tcgtagccgt gttcttcttt
    81061 caaccaagct ttgattggca cagggaatcc taatttcaca cgattcgacc attcttccgg
    81121 aagatgtttg ttcgcagctt gtctgaaaat atcttttgtg ttgttttgat tcaataaaaa
    81181 cttacttggt atttgttgag ccaatttcat gacttcgata tccaataaag gtactcggac
    81241 ttccaaagaa cttgccatag aaagtttatc tgcctttaac aagatatctt ttggcatcca
    81301 ttgatgaaga tcgacatatt gcatcttatt gacttcatct ttgattcctt ctgttttctc
    81361 gtaatgaaca gtcatgattt catccacaga tggtgctgtt tgatattttg gtgttaacac
    81421 ttctgatgct tctgattctt cgaacactcg agcatgaccg ataaaatatt cttttgcagg
    81481 agcaagtgat tcatataaat ggatccgtcc atggaagttt ttcatttttc cgattttacg
    81541 tcctaaatta tagcgtgttc cttttggtaa ttttttcaat ccttgagcaa tcacacggat
    81601 gaattttgaa ttcgtatgga atccatacgt ttggtatccc gcaaacagtt catccgcccc
    81661 ttctccagat tggactaccc gcacactttg tgaagcaaga tttgctaaaa agtacaaagg
    81721 tacgcaagaa ggatttgaat caggttcatc taagtgatac tggatcaaag ggaatgcttt
    81781 gaacgacatg tctccgtcga tcactgctgc tgtattatct aagtccaata gttcggttag
    81841 ttttctggct tcaatcgctt cattataggt tttatcatca aacccgattg agaaagaatg
    81901 atctggtttc aatacagagg ttacataact ggaatcaacc ccgctggata agaaagaacc
    81961 aacttctaca tcactgaccg tatgtgcttc aatagaagct tgaacggttt cgtcgatttt
    82021 ttcgatccat tcttgtcggg aatgcgtttc ttttgtttca aaatctgcat cccaatattg
    82081 ctgaatgtct atttttccat ctttatacgt ataataatgg ccttcaggca aacgatagac
    82141 atctttgaag aatgtttcgc cgtttaatgg agaatattgg aaagtcatat aaggtttcaa
    82201 cgcttctttg ttcaattctt tgttaaaatc aggatgaggc agaaaacttt tgatttctga
    82261 accaaacata aatgttccgt tcatttctgc ataataatat ggtttgatcc caaagtggtc
    82321 tcttgcaccg aataactcat tcttttcatt atcccaaata gcaaaagcaa acattccgcg
    82381 gatcttttgc aataattctg ttccccattc ttcgtaacca tgcaaaagga cttctgtatc
    82441 tgcatgtgtt tgaaaaacgt ggccagctgc aatcaattct tctctcaaag gctggtaatt
    82501 atagatttcg ccattaaaaa taatgatctt tgtccgatct tcattgtaaa taggctgagt
    82561 accgccctct aagtcaataa tacttaatct tctgaatcct aaagcaacat gtttatcgat
    82621 atattctcct gaactattag gtccacggtg aacgattcga tccatcattg tattaataat
    82681 tgtttttttg ttgtccttgt cgttaacaaa acctacaata ccgcacattt aactttcaca
    82741 tcctaatata aaattactgt ttatttcatt catatgcatc ttatcataga ataaatagcg
    82801 gacgaatatt ttctctctta caatttcgtc acgtgaaaac aaaagatgaa agtttctgtt
    82861 tttacagaaa ataaagtccc aatatctaat ttaccatgtt ttcttttttg aggctacctt
    82921 attctcttcg tgttaaaaaa atattagtgg atgctatttc caaaaaaacc agcaaaaaaa
    82981 gacacgacct aaagatcgtg t
//
LOCUS       DACEEZ010000005        68229 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20313_2017
            SAMN12639966-rid9879223.denovo.005, whole genome shotgun sequence.
ACCESSION   DACEEZ010000005 DACEEZ010000000
VERSION     DACEEZ010000005.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639966
            Sequence Read Archive: SRR10023158
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 68229)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 68229)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741683.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741683.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:44:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,016
            CDSs (total)                      :: 2,962
            Genes (coding)                    :: 2,889
            CDSs (with protein)               :: 2,889
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 36 of 73
            Pseudo Genes (incomplete)         :: 29 of 73
            Pseudo Genes (internal stop)      :: 18 of 73
            Pseudo Genes (multiple problems)  :: 8 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..68229
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639966-rid9879223.denovo.005"
                     /isolate="E20313_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(137..1528)
                     /locus_tag="IWO89_000367"
     CDS             complement(137..1528)
                     /locus_tag="IWO89_000367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-cystine transporter"
                     /protein_id="HAQ0455154.1"
                     /translation="MTTLITVLVVLVFAALLYVFYRMQKRHTKFSTRVFTALGVGIVF
                     GGLLQLIFGVDDKVVTQSMEWIGIVGQGYVSFLQMLVMPLVFISIIGAFTKMKVSEKL
                     GKISATVLTSLLGTTAIAALIGIGSTMLFGLQGAKFTQGAAETARISELATRQETVEN
                     LSIPQQILSFIPKNVFADLAGSRATSTIGVVIFAAFVGIAYLGVKRKAPKEAAFFSDL
                     VDSLYKIIMRIVTLVLRLTPYGVFALMTNVLATSDFAAIVNLGKFVVASYVALLAVLI
                     VHSLILIGAKVNPINYFKKAFPVLSFAFTSRSSAGALPLNIETQTKALGVDDATANFS
                     ASFGLSIGQNGCAGVYPAMLATIVAPAAGVNVFSLEFILMLVAIVTISSFGVAGVGGG
                     ATFASLIVLGAMNLPVAIVGLVISVEPLIDMARTAVNVSGSMVAGIVTSARIKDLDRD
                     VINDDSKVIDVNA"
     gene            complement(1741..2127)
                     /locus_tag="IWO89_000368"
     CDS             complement(1741..2127)
                     /locus_tag="IWO89_000368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1149 family protein"
                     /protein_id="HAQ0455155.1"
                     /translation="MEIRRQKEVVEAFHYDMRLPNQEVETDLKVGFSPLKETIENYPK
                     EHSIVGARLEFRLVFDTYVLSGSVSQINHILNRKIEGQNDVTQEEVDELVAPLFDIVR
                     RMSYEVTEIALDQPGVKLNFQSEKQE"
     gene            complement(2183..2806)
                     /locus_tag="IWO89_000369"
     CDS             complement(2183..2806)
                     /locus_tag="IWO89_000369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IA family hydrolase"
                     /protein_id="HAQ0455156.1"
                     /translation="MKNYIWDFDGTLFDTYPAMVDGAWQALKDFGISMDKKEIYFKMK
                     KYSTSYLINESNLNAREFNELFHRYEKESTEVSRPFPETKQVLEMLKDNGGRHFILTH
                     RLTESTWGLLKEHRLAHLIEEVVGIDQDFPRKPDPASLNYLIDTFHLERTDTMMIGDR
                     RLDIEAGKNAGVATCLYDIDHFLGEIPADYVVGNLNEILTLQSIIKK"
     gene            complement(2870..3538)
                     /locus_tag="IWO89_000370"
     CDS             complement(2870..3538)
                     /locus_tag="IWO89_000370"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-bisphosphoglycerate-dependent
                     phosphoglycerate mutase"
                     /protein_id="HAQ0455157.1"
                     /translation="MKLVLLRHGESEANFENYWTGWLDVALTEKGKEQAAVAGQKMLE
                     AGLQFDAVYTSVLKRAILTGQVALEEMGQLWLPIIKTWRLNERHYGDLVGKNKDKMKE
                     IYGKDQVKTWRRGYTEVPPLTAENRFDRRYDHLDPHLIPYGESLEMTVNRIIPLWQDH
                     LAPKLKDRQDLLVVGHGNSIRALTKYLEDVPEDQMDTIDIPNAQPIQYTLADDLTILD
                     KKIL"
     gene            complement(3550..3990)
                     /locus_tag="IWO89_000371"
     CDS             complement(3550..3990)
                     /locus_tag="IWO89_000371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0455158.1"
                     /translation="MMQTWLERLIRFQQNYHKLMQGRYAKFDQLNRGLLIAALVFSLL
                     SRWLPYRIGQLLFIVFFGWMIFRFLSKKIYPRLNENQRYLKYLDRVKEKYHQIKNKKQ
                     VKMDRKSYTFFECPNCHQKQRAPKGKGRIRVTCKTCGIKFETNV"
     gene            complement(4049..4721)
                     /locus_tag="IWO89_000372"
                     /pseudo
     CDS             complement(4049..4721)
                     /locus_tag="IWO89_000372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327855.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
     gene            complement(4906..5304)
                     /locus_tag="IWO89_000373"
     CDS             complement(4906..5304)
                     /locus_tag="IWO89_000373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-II family nitrogen regulator"
                     /protein_id="HAQ0455159.1"
                     /translation="MKDLVALNLHMIVTIVDGGMGEDVIDFSKEAGAAGGTILHGRGS
                     GVHDSGKFLGLDISPEKDIVLTLVPDSLTNKVMDAIGQGINIDIPGNGICFSIDVDKV
                     IGITKIHQYREIIGMQELLDENNDREKSES"
     gene            complement(5301..6074)
                     /locus_tag="IWO89_000374"
     CDS             complement(5301..6074)
                     /locus_tag="IWO89_000374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1538 domain-containing protein"
                     /protein_id="HAQ0455160.1"
                     /translation="MSFWQQVIDGIEIVTMEVILAVAPIVILFVLMNIISFRMRKKRF
                     FDIMKGFLITTIGLILFLHGVNMAYVPVGQHLGSAIAGLPYNYILVPLGFLMGFLVGF
                     AEPAIHVMIKQVEELSEGRIRSKIMLMVISIGIGAAVGLSMWRLLVGFSLYYFLIPGY
                     GLAFILGRKVDKIFLAMAFDNGGVATGPMCSTFILSMSVAIAAQIEGRDPIIDGFGVV
                     AMIALTPILSTLVLGYIYKRKDEKDRIEKERLQKLGESK"
     gene            complement(6071..6754)
                     /locus_tag="IWO89_000375"
     CDS             complement(6071..6754)
                     /locus_tag="IWO89_000375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1538 domain-containing protein"
                     /protein_id="HAQ0455161.1"
                     /translation="MLKNFKEVIAAILPMTVLIVILTFVFAPLEGEDLASFLVGAAIM
                     MIGMTLFLFGADYSMMEVGDLVGKYMIKKKSLTVLVSLGFAIGIVITIAEPSVQVLGQ
                     QVYDISDGEIGRVLLIGIVSVGTGVFLAFALLRVVFKLSYYQLMAIGYIGVLIASFFT
                     SSEFMPIAFDSGGVTTGPVTVPFILALAGGMTSMIKQKKNENDSFGMVGIASLGPILA
                     VMILGVIFQ"
     gene            complement(6921..7808)
                     /locus_tag="IWO89_000376"
     CDS             complement(6921..7808)
                     /locus_tag="IWO89_000376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HAQ0455162.1"
                     /translation="MKNMVEKETIERPNVKKQALELRSTIGSVLKYTALSILSFISIF
                     PFVWMVLGMTNTPVDITSGKIKIGSNLIINFQNLFSNELNFTRSLWNSAMIALVTTVF
                     ALLISSMAGYGFEIYQSKRKDLIFNILLLSMMVPFAALMIPLYRMFSQLNGTVLGINS
                     FLVVILPSVSTAFLIFFFRQNVKSFPKDLVEAARLDGLKELSIFFRVYMPTAKNTYAA
                     AAIITFMSSWNNYLWPLVALQSPDKRTVPLVLSAMGASYTPDYGMIMAGLVIATLPTA
                     IVFFVLQKQFVQGMIGSVK"
     gene            complement(7805..8674)
                     /locus_tag="IWO89_000377"
     CDS             complement(7805..8674)
                     /locus_tag="IWO89_000377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAQ0455163.1"
                     /translation="MKQKDLFKNGHAFLILPVILISIMVFIPMVLALITSFQSGPPVN
                     MTFNGLGNYQRMLTDATFKKAFFNTFLYLLVQVPIMLFLALFISNLLNDKKLKYKGLF
                     RTAIFLPCITSMVSYSLIMKSLFSQSGLVNNLLLGIGMIDTPIGWLTHPIWARVLIIL
                     SITWRWTGYNMIFFLSGMQNIDSEIYEAASIDGASKWQQFIKVTIPNLKPIILFTSIT
                     STIGTLQLFDEVQNITGGGPANATTTLSQYIYNLSYKFTPNFGYAAAVSFVIVSFIVI
                     LSIIQTKVGGESR"
     gene            complement(8811..10097)
                     /locus_tag="IWO89_000378"
     CDS             complement(8811..10097)
                     /locus_tag="IWO89_000378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter substrate-binding
                     protein"
                     /protein_id="HAQ0455164.1"
                     /translation="MNIRKLIAGVVCLAAASGFLTGCGSASSTADKEEKVTVWAWDET
                     FNIKAVNEAKKVYENEETEIEVVTMSQDDIVQKLNTALASGNTEGLPNIVLIEDYRIQ
                     GYLTSYPDAFANLSAIVKEDHFAPYKFAVNKVGDKIYGVPFDSGVTANFYRTDLMAEA
                     GYTEEDMENLKWDEYIQVARDVKEKTGKKIAEVNPSDLGRVRMIMQQAGEWYTSEDGE
                     TVTIQDNASLKYGLELFATLLKEDLVEQTSDWNAGVTAIQSGAVASSPTGAWYSSTIQ
                     GAENQSGKWKIAPIPALPENLQKAQASNLGGAGWYVIKGVAGEDSAKDFLEATFASNE
                     ELMGTLAKEIGLVSTMLSANEQEAYQEASEFYSGQKIFDDFSTWTSEIPEVNYGHETY
                     AIEAVVAEALHRIINGEETDKVLADTQKQVEAQLAN"
     gene            10370..10948
                     /locus_tag="IWO89_000379"
     CDS             10370..10948
                     /locus_tag="IWO89_000379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HAQ0455165.1"
                     /translation="MPIRVILIDDEQTILDGMKQLINWSEHGFQLIGTFSSPLKALDF
                     SLVHAIDIVVTDVMMPELNGIELSRLLKQQQPETQILILSSYDEFDMVKDSFKEGVAD
                     YLLKPRLSPDFFLNSLTAVSQKIKKKPNKSPITNRYEQICENLSQQIAGQTRPLLDPL
                     LFEHTLFFCYIPKNGHSVQNKALNKNSRSPIH"
     gene            10948..11877
                     /locus_tag="IWO89_000380"
     CDS             10948..11877
                     /locus_tag="IWO89_000380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAQ0455166.1"
                     /translation="MDSDDSFTIYPVSTFSEESGYVINTDSKDTLRKFVQKFRQEVFP
                     GDFFVVSDSFAIQDINEIFQEIRNCTKGQRFYHKGKNLAYQHELLPLLTNHEQQASSY
                     LTKIVHKDYALAVKELENFFTTILEQPIDPAYLKHEAINRFYALLNALADEHVGNEEL
                     SLLKIALPSLLADAEYFEDFSILVKETLEKLTHFSANAKKDNFDILTLIYEFVQENYE
                     KEINLQLLSEKYHFSYSYLSAIFTEKFGINFSKYLKKVRISKAKLFLTETRLTLTEIC
                     EKVGYTELGYFSRVFKEETGMTPSQYRKGTFVK"
     gene            11874..13610
                     /locus_tag="IWO89_000381"
     CDS             11874..13610
                     /locus_tag="IWO89_000381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HAQ0455167.1"
                     /translation="MRKKIKEHELFAKLLFILFIGLFLQAIVISLFIYHRSRDAYIQL
                     FNQSNDVVLKKIQSEFESLNDTIENTLAAFDSNPAVKSYFSNDPAQHMEQLQQLRTIQ
                     KMNDSLSKIHPMIDYDVLIFGENGRTFVGNDMLTAVSADSFFQSAIAQRVNERAADTQ
                     MLFAHHGLTLRDKKAPSVFFVKKLKNTLNHVYGYAVLSISSKQLANLFQSVVNPEISK
                     ISFMNEEQTIIASNEENTIGSRSMIFKKLTVGETTISNGQQLTRLPLYRQDCTLLSQV
                     DLHSLSNQMGVIFPIILYNIVTLAFAGSLVFMYLDRHTKSIYRLIDSLKRLEEDGLKA
                     TVPVQGSHEVQLLGNTINQLLSNAQHHYEQTIQDEKKKRSLEIQTMQAQIQPHFIYNT
                     LTSLKFLIWQKKTDEAVQGIDSFITLLRSTIGKKEEVIPVKEEIKSVQSYIDLLSMRY
                     GERISAKIMIPDELQSLSMPNMILQPIIENAYLHAFHNKTQGFITVYGKTKNQLLLFE
                     VIDNGDGFDTENTKQKNDHFSGIGISHVDERIRLMYGDAYGLTIQSVIGTGTTVTICL
                     PLLPKNQSEYQK"
     gene            complement(13673..14509)
                     /gene="pheA"
                     /locus_tag="IWO89_000382"
     CDS             complement(13673..14509)
                     /gene="pheA"
                     /locus_tag="IWO89_000382"
                     /EC_number="4.2.1.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydratase"
                     /protein_id="HAQ0455168.1"
                     /translation="MNVSYLGPESSFTYQAACQLFPTEQLTAYASIPVCLRALFRKQV
                     DLAVVPVENSLEGAVHHTIDLLSKHPEVEVKSEIVLPIKQQLLGNPATKITKILSHPQ
                     ALAQSQQFLETHYPNVPLVATESTTAAAMYVAEHPKEDAAAIASLETAQHVGLEILAE
                     NIQDNELNQTRFWIVGDRKMTSQQSAPVKMSVILTLPANRPGMLHKMLAAFGWREINL
                     SKIESRPLKTSLGEYFFVIDLLLDRPMTLVENALQEIKMLGGESQILGCYPVLTVEET
                     RR"
     gene            complement(14506..15015)
                     /locus_tag="IWO89_000383"
     CDS             complement(14506..15015)
                     /locus_tag="IWO89_000383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase"
                     /protein_id="HAQ0455169.1"
                     /translation="MASILLIGFMGAGKTTIGKGLAQCLQKEYVDLDTKIEEHIQLSI
                     AEYFHYYGEKSFRKVESDILRKLSNEDKIIATGGGIVQSAENRRFLKTQPIVLYLEAE
                     ADCLVDRIQQDETSIRPLALGKTREEIKALLAQRLSWYEESATHRINTTNQTPEKIID
                     TIIERIKSI"
     gene            complement(15008..16303)
                     /gene="aroA"
                     /locus_tag="IWO89_000384"
     CDS             complement(15008..16303)
                     /gene="aroA"
                     /locus_tag="IWO89_000384"
                     /EC_number="2.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HAQ0455170.1"
                     /translation="MQLLQQIHGLRGTVRIPADKSISHRSIMFGAIAEGTTTIQNFLR
                     AEDCLSTLHAFQQLGVEIEEEEEVIKIHGRGSHSFVQPTESIDMGNSGTTIRLLMGIL
                     AGQPFTTTLFGDASLSKRPMGRVMEPLREMGADLQGEENDQYLPITVTGTRSLSPIRY
                     NMPVASAQVKSALLFAALQAEGTSVIVEKERSRNHTEEMIRQFGGRITVEDKTIIVTG
                     PQKLTGQQITVPGDISSAAFFLAAGLLVPESQLLLKNVGVNPTRTGILDVLEEMGAAI
                     TQTNHNEQNQSADLSVKTSHLKKATIGGEIIPRLIDELPILALVATQAEGITIIKDAE
                     ELKVKETNRIDAVAEELQKMGAKIKATADGLIIHGPTPLHGAKVSSRGDHRIGMMLQV
                     AALIADGPCELEGAEAVSISYPAFFDDLAELVSGGEAHG"
     gene            complement(16316..17407)
                     /locus_tag="IWO89_000385"
     CDS             complement(16316..17407)
                     /locus_tag="IWO89_000385"
                     /EC_number="1.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydrogenase"
                     /protein_id="HAQ0455171.1"
                     /translation="MAQHVFIVGLGLIGASMALCIRQTNPELIVVGWDSQSKTREQAV
                     EQQIVDYVASDFETGAEQADVILLAVPVRTTLAYLDVLERINLSDHVIITDVSSTKQQ
                     VVTSAEQKNLRFVGGHPMAGSHKSGIQAADAKLFENAYYIFTPLEKTKKDVEKLQELF
                     CGTKAKYVTLTAREHDRITGMLSHFPHILAAGLVNQAEQFNQEYPRAKQLAAGGFRDI
                     TRIASSDPVMWTDILLSNKQILLERLADWQQEMTQIAEWIMTENQSEIFSFFNRAKES
                     RDQLPVHKQGAIPAFFDLFIDVPDEPGVIAEVTGLIGKAGVSLINLKIQETREDILGI
                     LQISFKNQQDLLQAKRIIMSNTSYHCWIK"
     gene            complement(17424..18590)
                     /gene="aroC"
                     /locus_tag="IWO89_000386"
     CDS             complement(17424..18590)
                     /gene="aroC"
                     /locus_tag="IWO89_000386"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016175098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HAQ0455172.1"
                     /translation="MRYMTAGESHGPELTAIIEGLPAGLPLSEEEINHELARRQAGYG
                     RGGRMKIEKDRVRITSGIRHGKTIGSPVTLVVENKDWKNWTTVMSVAEVPEKDQSLRR
                     VARPRPGHADLVGGMKYHHRDLRNVLERSSARETTMRVAIGAIAKKILEELDIIVAGH
                     VICLGGIQAEIPGNISIQEIKEISEASEVRVVDPAVEQRIKDLIDQTKKNGDTIGGIV
                     EVLVGGVPAGLGSYVQWDTKLDAKIAQAVVSINAFKGVEFGVGFTSGSLPGSEVMDEI
                     LWEEERGYFRRTNNLGGFEGGMTNGEPLVIRGVMKPIPTLYKPLQSVDIDTKEPYKAS
                     VERSDSTAVPAASVVAENVVATVVATELLSKFPSDSLVELKAAVKEYRDYLKHY"
     gene            complement(18592..19656)
                     /gene="aroB"
                     /locus_tag="IWO89_000387"
     CDS             complement(18592..19656)
                     /gene="aroB"
                     /locus_tag="IWO89_000387"
                     /EC_number="4.2.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydroquinate synthase"
                     /protein_id="HAQ0455173.1"
                     /translation="MLTVHVDNQKYDIHIEKGALEQAGSWLQTIWRPQKVAVITDTNV
                     APLYAEKIMFQLRQANFEPVLSIVPAGETSKSLEQAAELYDFLADNDFTRSDGILALG
                     GGMVGDLAAFVASTFMRGIHFVQIPTTLLAQVDSSIGGKTAVNTNKAKNLVGTFSQPA
                     GVLIDPEVLKTLPVRRLREGIAEIIKSAAIADATLWKQLNELKDEKDLLEHAVPIITA
                     TLEIKRKVVEEDVFDQGNRLILNFGHTIGHAIENTAGYGVVSHGEGVAIGMVAITSHA
                     EKIGLSPTGSTAALERILQKFHLPITYDLSHPDELFHAITHDKKARGSQLNIILLEKI
                     GQAKIVPVPTETFKDYLGGL"
     gene            complement(19689..20711)
                     /gene="aroF"
                     /locus_tag="IWO89_000388"
     CDS             complement(19689..20711)
                     /gene="aroF"
                     /locus_tag="IWO89_000388"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase"
                     /protein_id="HAQ0455174.1"
                     /translation="MIVIMKKEATEEQIKKVVDHIEKEGFKVSIDQGTERVVIGLKGD
                     TRSLQEGAFTRYEGVENAVRILNTYKLTSREFHPGNTVVDVDGVKIGDGSFVTMAGPC
                     SIEGLDQIRECARMAKAGGAKILRGGAFKPRTSPYAFQGLEEEGLKYIRQAADEFGMK
                     VITEVMDEGHIDMVAEYSDILQIGARNMQNFKLLSAVGKTGKPVGLKRGISGTINEWL
                     NAAEYIAVEDKSPVIFIERGIRTYETATRNTFDLSAVPLMKKLTHFPVIVDPSHGTGI
                     WELVPPMARAGVASGADGMIVEIHPDPANAWSDGPQSLNEKTYSRMMKEVAIMKEAME
                     KIQTLD"
     gene            complement(21370..22344)
                     /locus_tag="IWO89_000389"
     CDS             complement(21370..22344)
                     /locus_tag="IWO89_000389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase small subunit"
                     /protein_id="HAQ0455175.1"
                     /translation="MKNTVNKSHMDTEKVAIVFGDCTLGVKSGNTHYIFSYTRGGLES
                     LNKNGKEWLYRETLPVFWRATTDNDRGSGFGFKSGMWLGAGLYPKVKEIEVLVDNKKI
                     DLPIAPVNNQYSSNEYAETVEIIFTLEINTVPKTQVTISYQIAGEGKMLVNMHYYGQK
                     GLPELPALGLRFIMPTKAKFFEYEGLSGETYPDRMAGGVEGIYKVAGLPVSPYLVPQE
                     CGMHMETKWVELTRNQTLDNTDKDQEDFSLRFEQQDTPFAFSALPFKAEELEQATHIE
                     ELPPERRTVLTIFAKVRGVGGINSWGADVESPYRISSEEDHEIGFIIQ"
     gene            complement(22325..24208)
                     /locus_tag="IWO89_000390"
     CDS             complement(22325..24208)
                     /locus_tag="IWO89_000390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase"
                     /protein_id="HAQ0455176.1"
                     /translation="MKANIKWLDDPQVFRVNQSNAHSDHVCYATREEVETTSSLKRSL
                     NGSWRFCYSPNPSVRPKTFYKKDFDACSFDEITVPSHIELANYDKIHYINTMYPWEGH
                     VFRRPPFTTSPEKEAEGIFSAAKDNPVGSYLTTFRLEEGLKGKQISIHFAGVEQAMFI
                     WLNGHFIGYAEDSFTPSEFDLTPYLEEENILAVEVYKRSTAAFLEDQDFFRFFGIFRD
                     VFLVARGQNHLEDLWIKPRLDDKCQNGKLEVDLSFEQVAKNSQLNVAILDPDGKEIYQ
                     QQYPLKEQMTIRIDGFENVALWGHFQPNLYKLELETIDEEKRVTEYIDYSFGFRKIEI
                     KDKIIYFNGKRMIINGVNRHEWHPKKGRAITIEDMEKDLAIIKNAKINGVRTSHYPNQ
                     ITWYYLCDQAGIHVMAETNLESHGSWQKMGAFEPSYNVPGAVPQWQAAVLDRAKTNFE
                     TLKNHPSILFWSLGNESYAEENIRQMNDYFKKVDPDRLVHYEGNFPNPAFEDSISDVK
                     SCMYAPPEEIIDYLENKAEKPFILCEYMHDMGNSLGGMDSYMTLLDRYEMYQGGYIWD
                     FKDQAIQVTDPITGREVMRYGGDFDDKPSDYEFSGNGIVFADGQEKPAMQEVRYYYEK
                     YSK"
     gene            complement(24226..26451)
                     /locus_tag="IWO89_000391"
     CDS             complement(24226..26451)
                     /locus_tag="IWO89_000391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-galactosidase"
                     /protein_id="HAQ0455177.1"
                     /translation="MDITHRKTDKNFILFDEKSRVFHLRNTFLSYLIKIEESNVLAHI
                     YFGKPVKQYKDNKNYPRRDRGFSGNVPLNPDRSLSKDTLPQEYSSHGSMDFRTPASII
                     QRKNGSDLLDLRYDSHYITDGKPEIEGLPQTYVMDKSEAQTLVISLKDRETAIYFDLF
                     YTIFTDRAVITRSVKIRNETGETIKLEKAASFQLDFAHTRRFDEVIALPGAHVNERQI
                     SRQSVLSGTKVFESRRGTSSHHMNNFIALVHHHTTENTGEAIGLQFVYSGNHSFELEK
                     DQINQLRVVGGINSHRFSWELNAGQSFQTPEMILSYSSQGLNKMSQIHHELLRERIAR
                     GRHQFAERPILVNNWEATYFDFNSEKIKAIIDEAKELGIEMFVLDDGWFGKRDADNSS
                     LGDWFEYEGKLTNGLREIADYAHSKGLKFGLWFEPEMISVDSELYRTHPDFLMQEPGR
                     MPSASRSQHVLDFTRLDVRQTIEKQMRKILDTIPLDYIKWDMNRSLSDVYSITLDPQR
                     QGEVAHRYMLGLYELLEHLTTDYPEILWEGCSGGGGRFDAGFIYYMPQSWTSDNTDAV
                     ERMKIQYGTSLAYPISSITAHVSAVPNHQTDRSTSLKTRGETAMSAVFGYELDLTKLS
                     PEEKKQVKDQIISYQTIRPVIQYGHYYRLASPFEENIAAWMFVSPKQDEAIVFLGRIL
                     ASAQPAFHEVYLMGLDDEALYQEQTSKRIFSGAELMTVGLYFPDFQGDFQTELLHFKK
                     L"
     gene            26566..27600
                     /locus_tag="IWO89_000392"
     CDS             26566..27600
                     /locus_tag="IWO89_000392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family transcriptional regulator"
                     /protein_id="HAQ0455178.1"
                     /translation="MVGIRDVARKANVSPATVSRVLNNDQTIHVAQTTRQRIVEAAAA
                     LDYDISTRKYIKKRMPSIGVISTIGKASEEQDIYYKHLRVGMEEEARRLHLGMNRIYN
                     LSDNPKQWEDLDQLGAVIVVGTVTKESITALLKQNMQIVVVDNPDIQQEVDMVYADLE
                     RMTKTVLELFLENGHKRIAYIGGYKVDMDEFGGKQISINEKRMRAYQHFMIDHHLEKY
                     MSYHLGGWTEKQGEVLATNLLENESPLPTAILVGSDPMAIGVYRALKQKNIQIGEEIV
                     VASFDNIETIANLSPGLTSVQINAKSIGQAAVRMALERIDGIRDEPIILTYPARLIVR
                     ESFIPRTKRY"
     gene            complement(27667..28680)
                     /locus_tag="IWO89_000393"
     CDS             complement(27667..28680)
                     /locus_tag="IWO89_000393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HAQ0455179.1"
                     /translation="MKKKILKFIGWFFGIILGIAFAIFLAFQLSPRPGAFLINHMFSN
                     TVQITDKKTFDQAEKNVAKKTDLTYESKYKENTYDIYYPKNSKGPVPVLFWVHGGGFV
                     GGDKSGVKEFATKLVSDANIAVVAMNYELAPDSEYPNQVLQVNELIKDLLAEEKDDKL
                     LDMEQLFFGGDSAGSQIALQYAAIQTNDAYAKQMKIKQLLPKDSLKGTISYCGPVDLK
                     LTAQQHSDDRFMKFFVKTVAWSLIGTKDWKTSEQLKEASVADHVSKEFPPTYITDGNA
                     YSFQEQGQALENRLTELKVPVQSLFYTDTKKEITHEYQFDYTLKESQNCYQQTLDFVN
                     KYK"
     gene            complement(28758..28946)
                     /locus_tag="IWO89_000394"
     CDS             complement(28758..28946)
                     /locus_tag="IWO89_000394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine synthase"
                     /protein_id="HAQ0455180.1"
                     /translation="MEEHQQVIDRIYEATRYLPLDRLCLSPQCGFASTEEENVLFEEQ
                     WKKFLLSKALQKKFGENH"
     gene            complement(29386..29514)
                     /locus_tag="IWO89_000395"
     CDS             complement(29386..29514)
                     /locus_tag="IWO89_000395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016175013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HAQ0455181.1"
                     /translation="MDQKEKMKELIKKKQGGGRSKQMETPKNDRKNMRKGPKIFNK"
     gene            complement(29517..30890)
                     /gene="rlmD"
                     /locus_tag="IWO89_000396"
     CDS             complement(29517..30890)
                     /gene="rlmD"
                     /locus_tag="IWO89_000396"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HAQ0455182.1"
                     /translation="MTEQQIVKKNERYTVEIVDLSYEGMGVAKIDGYPLFIENALPGE
                     EVEILVVKAGNKFGYGKVETFITQSPDRQPVENNALLRIGIAPLAHLKYEQQLVFKQK
                     QVEHVMAKIAKMPEVPVLPTVGMAEPFGYRNKAQIPVRRIDGQLATGFYKKNSHDLVP
                     IEDFYIQDPVIDQAIKVVRDILQRFQVRGYNEEKNEGQLRHIIIRRGHYSHEMMVVLV
                     TRKEKFFKSKEIAEAIHEELPEVVSVIQNINEEKTNVILGDKEKVLYGRDYIEDQLLG
                     KTYRISAKSFYQVNTQQAEYLYQTAIEFAELDKKDIVIDAYSGIGTIGLSLADRVDKV
                     YGMEVIPEAIEDAQFNALNNKIENVHYEVGKAEKVMKKWQEKGIKPSVIVVDPPRKGL
                     DHHFIESAIEMSPERIVYISCNPATFARDAKLFAEAGYAVKKVQPVDLFPQTHHIELV
                     ASFVKGE"
     gene            complement(31001..32056)
                     /locus_tag="IWO89_000397"
     CDS             complement(31001..32056)
                     /locus_tag="IWO89_000397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HAQ0455183.1"
                     /translation="MKKARVIYNPTSGKELLKKNLADILQALEEAGFEASAYATTPEP
                     DSAKNEACRVAELGFDLVVAAGGDGTINEVVNGIAPLEHRPKMAIIPAGTTNDYARAL
                     KIPRDNIKAAAEVIKKHQTVKMDIGLSDNSYFINIAAGGYLTELTYEVPSELKSIFGY
                     LAYLAKGAEMLPRVKPIKMRLKYDEGEYIGNASMFFLGLTNSVGGFEQIAPDAKLDDG
                     KFSLIIVKTANIFEILHIAALMLNGGRHVDDPRIIYTKTSSLFVETEDDINRPVMINL
                     DGEYGGEAPMHFQNLHQHIEFFANTDQIPDEAITGTDEEELEVVSKEFVKEVERLTDE
                     DIDGDGKIADETPNQKN"
     gene            complement(32066..33496)
                     /gene="gatB"
                     /locus_tag="IWO89_000398"
     CDS             complement(32066..33496)
                     /gene="gatB"
                     /locus_tag="IWO89_000398"
                     /EC_number="6.3.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatB"
                     /protein_id="HAQ0455184.1"
                     /translation="MNFETVIGLEVHVELKTNSKIFSPAPAHFGAEPNSNTNVIDWGY
                     PGVLPVMNKAALEFGMKAALALNCEISKDTHFDRKNYFYPDNPKAYQISQFDQPIGHD
                     GWIEIEVEGKKKKIRIERVHLEEDAGKNMHGIGGYSYVDLNRQGTPLIEIVSEADMRS
                     PEEAYAYLEALRSIIQFTEVSDVKMEEGSMRCDANISLRPYGQEEFGTKAELKNLNSL
                     SFVKKGLAFEEKRQAKVLLSGGEIQQETRRFDETTNKTVLMRVKEGSSDYRYFPEPDI
                     PKFVIDDEWIQKVKASLPEMPASRRERYIREFGLPEYDAMVLTLTKEMSDFFEAVLEN
                     GADAKQASNWLMGEVSAYLNSEKLELAETKLTPENLAGMIRLIADGTISSKIAKKVFR
                     ELITNGGEAQEVVEKNGWVQLSDPNKLLPIINEILDNNQQSVDDFKNGKDRAVGFLVG
                     QIMKATKGQANPGVVNKLLKDELAKR"
     gene            complement(33493..34965)
                     /gene="gatA"
                     /locus_tag="IWO89_000399"
     CDS             complement(33493..34965)
                     /gene="gatA"
                     /locus_tag="IWO89_000399"
                     /EC_number="6.3.5.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatA"
                     /protein_id="HAQ0455185.1"
                     /translation="MTELHNRSLEELHHMLVSKEITVQDLTKTTFERIKETEPEVEAF
                     ITLNEEKALEQAKALDEKGIDESNVLAGIPIGIKDNIVTKDILTTAASKILYNFEPIY
                     DATVMDKIYQADMIPVGKLNMDEFAMGGSTETSYFKKTKNAWDPTKVPGGSSGGSAAA
                     VAAGQIPVSLGSDTGGSIRQPAAFNGIVGMKPTYGRVSRFGLIAFASSLDQIGPMTRT
                     VKDNALALTAISGYDEKDGTSSGVSVPDFAEGLTGDIKGMKIALPKEYLGEGVAPGVK
                     EAVLKAAETFRSLGAVVEEVSLPHSKYGVAVYYIIASSEASSNLQRFDGIRYGYRSEN
                     VKSLDDVYVNSRSEGFGEEVKRRIMLGTFSLSAGYYDAYFKKAGQVRTLIKRDFEKVF
                     ADYDLIIGPSSPTVAFGIGENINDPITMYMSDILTIPVNLAGLPGMSVPAGLSEGLPV
                     GLQIIGNYFDEKTMYQAAYAFEQATEFHKQQPAILGGKDE"
     gene            complement(34965..35270)
                     /gene="gatC"
                     /locus_tag="IWO89_000400"
     CDS             complement(34965..35270)
                     /gene="gatC"
                     /locus_tag="IWO89_000400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002378010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatC"
                     /protein_id="HAQ0455186.1"
                     /translation="MAISEEQVKHVAKLAKLAFADEELASFTDQLGKIIDMVEMLEEV
                     DTEGVPFTSNVVETVNVMRQDRAQAGWNRDELLKNVPETEDGFIKVPAIIDNGEAGA"
     gene            complement(35376..37412)
                     /gene="ligA"
                     /locus_tag="IWO89_000401"
     CDS             complement(35376..37412)
                     /gene="ligA"
                     /locus_tag="IWO89_000401"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HAQ0455187.1"
                     /translation="MEHEMTIDEAAKRAEELRTRLNQWSREYYVEDKPTVEDYVYDKE
                     YAELVAIEEQYPDLITSDSPTQRVGGKVLEGFEKVTHDIPLYSLNDVFSKEELIAFDQ
                     RVQKAVGRVVDYCCELKIDGLSVSLRYEDGNFVRGATRGDGTVGENITENLKTVRSVP
                     IKLKEPMNIEVRGECFMPKRSFVQLNQDREAEGKDIFANPRNAAAGSLRQLDSKITAK
                     RNLDTFLYTVADFGPMQAKTQYDALEELEKIGFHTNREKRLCHSIDEVWSYIEEYHDK
                     RVDLPYEIDGIVIKVNEFSLQDQLGFTIKAPRWAAAYKFPPEEVETLIENIEWTVGRT
                     GVVTPTAIMTPVRVAGTTVSRASLHNGDYIKLKDIRLKDTVLIYKAGDIIPEVSQVVL
                     DKRPKDSEEYQLPTHCPVCGSELVHLDEEVALRCINPKCPAQMKEGLNHFVSRNAMNI
                     DGLGPRVLEQMYDKKLVADVADLYKLTEEELLTLDKIKEKSANNILTAIDNSKDNSVE
                     RLIFGLGIRHVGAKAAKILAEHFGDLETLSKSDYESIIALDTIGDIIADSVVTYFSNE
                     EVHELMNELKQAGVNFEYKGLRNAQLQEVESPFKEKTVVLTGKLTRFTREEAKETIEN
                     LGGKVTGSVSKKTDIVVAGEDAGSKLTKAQELGIEVWTEDQMADALAKSRSVEE"
     gene            complement(37429..39663)
                     /gene="pcrA"
                     /locus_tag="IWO89_000402"
     CDS             complement(37429..39663)
                     /gene="pcrA"
                     /locus_tag="IWO89_000402"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase PcrA"
                     /protein_id="HAQ0455188.1"
                     /translation="MSSKAELLNGMNPRQKEAVLHTDGPLLLMAGAGSGKTRVLTHRI
                     AYLIEEKEVNPWNILAITFTNKAAKEMKERVNAILASGGEDVWVSTFHSMCVRILRRD
                     VDFIGYNRNFTIIDSSEQLTLMKRILKELNIDPKKYDPRSILGTISQAKNSLQTPQDF
                     AKMQGSYYEEIAAKCYAAYQKELQYNQCMDFDDLIMNTIRLFEEHPDSLTYYQNKFHY
                     IHVDEYQDTNHAQYTLVNLLAGRFRNLCVVGDADQSIYGWRGADMQNILDFEKDYPDA
                     AVILLEQNYRSTKNILSAANQVIENNSNRKPKNLWTENKEGNKITYYRADNERDETRF
                     IVDRMQEEIRSNHRNYGDFAILYRTNAQSRVMEETLLKANIPYKMVGGHKFYDRKEIK
                     DILAYLNVLVNPQDSISFERIVNSPKRGIGPGSIEKLRSFASLHEWPLLEAAQNVDLA
                     NIGGKAGQQLGAFGEMIQEVTQMIPYLTVTELTKEVLDRSGYLEDLKIQNTLEAQARI
                     ENLEEFLTVTQEFDKQFEQQNEEDADAPEEKLTVFLNDLALVSDIDNLEEDASQVTLM
                     TLHAAKGLEFPVVFLIGLEEGVFPLSRALMEESELEEERRLAYVGITRAEEALYLTNA
                     FSRTLYGRTQYNRPSRFVEEIDQELLEIEGMRPTPKKTPVFAKKTAYSYKQPETAVVP
                     SKSATGGENNSWKPGDKVKHKKWGLGTVVRVSGTSKDLELDVAFPSQGVKRLLAAFAP
                     IEKA"
     gene            39818..41377
                     /locus_tag="IWO89_000403"
     CDS             39818..41377
                     /locus_tag="IWO89_000403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ClC family H(+)/Cl(-) exchange transporter"
                     /protein_id="HAQ0455189.1"
                     /translation="MEKKGKIKKLDKTQIYFILKGIFVGLLTGIVVSLFRLSIEKLSD
                     LVRSIYEMSREQPIYLIGLAICCILAAFFVGYLVKNEPDIKGSGIPQVEGQLRGELSM
                     NWFSVLWKKFIGGVLSVGAGLFLGREGPSIQLGASIGQGAGQLFRSPSSEEKILISSG
                     ASSGLAAAFNAPIAGLLFVLEEIHHSFSPLVWLTSFASAITANFVSLYFFGLRPVLYL
                     GDLPSLPLKYYGSLVILGVLLGILGFIYQNVLLALPKFYNRLPLPAYFHGFVPFLLIL
                     PIGYLWPSTLGGGNQIVLAFGHASLPIIMIIGLFVLRFVFSMISYGGGLPGGIFLPIL
                     TLGALIGSFYGNIMVDFGMDPIYIKDFVVIAMAGYFTAIGKAPLTAIILVTEMVGSLN
                     HLMPLGLAALVAYIVNDLLGGNPIYESLLERLLGNTQTASLKGARTTFDFPVTAESSL
                     DGLMVRDFNWPKNMLLISIRRGNQEILTHGDTIMNVGDILVILTDEANLPRIKQEIEA
                     KSSITNLKQSF"
     gene            complement(41488..43419)
                     /locus_tag="IWO89_000404"
     CDS             complement(41488..43419)
                     /locus_tag="IWO89_000404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIA"
                     /protein_id="HAQ0455190.1"
                     /translation="MNIKDLMVKEAMIMDLQATDKKGAIDEMVQKMFDAGRISDIETY
                     KEGILAREAQTSTGLGDGIAMPHAKNSAVKEATVLFAKSQKGVDYEALDGQPTYLFFM
                     IAAPEGANDTHLQALAALSRLLIDADFVGKLKEANTPEEVQDLFQTAEEQKEAEEKAE
                     QETQAAEASAHPERKFVVAVTACPTGIAHTYMAEDALKKKAKEMGVEIKVETNGSEGI
                     KNRLTAEDIARAEGVIVAADKKVEMNRFDGKELINRPVSDGIRKPEELINLALSGTAP
                     VFHGDGKESSSEEGNADGTIGQRIYKDLMNGVSHMLPFVIGGGIAIALSFMIDQFIGV
                     PHDQLSNLGNYNQAASWFNQIGQAAFGFMLPVLAGFIASSIGDRPGLIVGFAAGALAN
                     TGGAGFLGALIGGFLAGYVIVFLRKLFKNLPKSLDGIKTILFYPVFGLLITGFLMLLV
                     NVPMKAINDALNGFLTSMSGSNAALLGALLAGMMAADLGGPINKAAYVFGTATLATTV
                     ATGGSVVMASVMAGGMVPPLAIFVATRLFKNKFSKTDQDAGLTNIVMGLSFVTEGSIP
                     FAAADPIRAIPSFIIGSALTGGLVGAFGIKLLAPHGGIFVVFLLSHPVMYLVFIAIGA
                     VVSGVIYGLLRKAPAVAEA"
     gene            complement(43437..44351)
                     /gene="pfkB"
                     /locus_tag="IWO89_000405"
     CDS             complement(43437..44351)
                     /gene="pfkB"
                     /locus_tag="IWO89_000405"
                     /EC_number="2.7.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-phosphofructokinase"
                     /protein_id="HAQ0455191.1"
                     /translation="MIYTVTLNPSIDYIVRVDGLKLGDLNRMKEDFKLPGGKGINVSR
                     ILKRIDSQSTALGFLGGFTGEFISKWLEKEQIQSLFTTVKEDTRINIKLKSDTETEIN
                     GLGPSISDEEIDELKQALGTVGQGDIVVLSGSAPASLRKGFYQELIDIIREKGADFVI
                     DTTGDDLKNALVKKPLLIKPNNHELAELYNTTFQSVEEIIPYGKKLLAEGAQNVLISM
                     AGDGALLFTEQGTYKTNVLVRPLKNSVGAGDSMIAGFIGAYSRTKDPIEAFKWGVACG
                     SATAFSDDLAARELIDELLPEVEITKID"
     gene            complement(44348..45100)
                     /locus_tag="IWO89_000406"
     CDS             complement(44348..45100)
                     /locus_tag="IWO89_000406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HAQ0455192.1"
                     /translation="MLTEERRQKILDLLEQQKIVKSQDLVNLLNASESTIRRDLQELE
                     NSGFLERVHGGAKKEQLLNFEQNMSEKSLKNVHEKQQIAKSAAETINDEDVIYLDAGS
                     TTLEMIPFLQGKQITVVTNSVQHAANLVDLELATIILGGTIKLATNAVLGSNAIEQLK
                     NFHFNKAFMGMNGAHLEQGFTTPDPEEAAVKRLAMKNSEETFVLIDHTKFNQLSFTSV
                     APLEAATIITERCPLEFVNEFREKTTIKEAIQ"
     gene            complement(45259..45468)
                     /locus_tag="IWO89_000407"
     CDS             complement(45259..45468)
                     /locus_tag="IWO89_000407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0455193.1"
                     /translation="MDKQLYYRIMVPASLLSVCLFFVPNFPIRSVIGFLVMFGGMGLY
                     YYLLHKERKPASVYMKKTRSENKKR"
     gene            complement(45479..46807)
                     /locus_tag="IWO89_000408"
     CDS             complement(45479..46807)
                     /locus_tag="IWO89_000408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0455194.1"
                     /translation="MIGEDKLLMTIQLTDLNEEEKKQEISDWAILTRLLIQPTFIRSF
                     TQQADPYDLRKLQEKIMLASVRDESWLVVGNDENECSFRLEDNQLLIKNVLSISVFKE
                     KQFLIRDFIQKKMIAHGVFAYMRAYSEFIYHNTKQISQRLLFEKKDEIEHLPKMKQQN
                     GEVVVDCNQFPGYDLFYQGLCFTSCWEMYYSRYYHQIIPKPIFLDIQQVEKVKELENE
                     VICIQLYRDPFNWQKPNNQMFQSYFRDQLGFDHLAWDNGVGLLKPPFVEYIYTDHTIQ
                     SVQYQNAQMQPVPKKNASFFVTKSYDIQQGDYKERRVRGTLNAQAYFPWVDENRSRMM
                     CYKVIDPTVALDNGIEAYCYYIREYLEVEVTDEKYQEYLLSLRIYVPSESLSELPLKE
                     IKHRLSDVTFKRIKKKRRSIQFDVKKGEQHLRVQFLDYRELEQLITLQKI"
     gene            complement(47172..47759)
                     /locus_tag="IWO89_000409"
     CDS             complement(47172..47759)
                     /locus_tag="IWO89_000409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihA family ribosome biogenesis GTP-binding
                     protein"
                     /protein_id="HAQ0455195.1"
                     /translation="MKVHQAEIIISAVAPKQYPDTQLPEIALAGRSNVGKSSFINTLI
                     DRKNLARTSGKPGKTQTLNFYLIENALHFVDVPGYGYAKVSKTERAKWGKMIETYLTT
                     RDQLRAVVSLVDLRHKPSQEDIQMYQFLKYYDIPVIVVATKADKIPRGKWNKHESEIK
                     KALDFDRNDTFILFSSVTKQGKEEAWAAIEQAIES"
     gene            complement(47842..49092)
                     /gene="clpX"
                     /locus_tag="IWO89_000410"
     CDS             complement(47842..49092)
                     /gene="clpX"
                     /locus_tag="IWO89_000410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpX"
                     /protein_id="HAQ0455196.1"
                     /translation="MYDNPSSNETVRCSFCGKTQEEVKKIVAGPGVYICNECIDLCKE
                     IIDEEFYEEAVREFTEVPKPLEILEVFNNYVIGQDRAKRSLAVAVYNHYKRVNQQAQE
                     NTDDVELQKSNICLIGPTGSGKTFLAQTLAKTLNVPFAIADATSLTEAGYVGEDVENI
                     LLKLLQSADYNVELAEKGIIYIDEIDKIARKSENVSITRDVSGEGVQQALLKILEGTV
                     ASVPPQGGRKHPHQEFIQIDTTNILFIVGGAFDGIETIVKNRMGEKTIGFGTQNQKLS
                     EDESVMQHIIPEDLLKFGLIPEFIGRLPVMAALEKLTTDDLVRILTEPKNALVKQYQK
                     LLSLDNTELEFEPEALRAIAKKAIERNTGARGLRSIIEEIMMDVMFDIPSNENIEKVI
                     ITKEAAELSGEPTVIYKDSEKKAG"
     gene            complement(49339..50628)
                     /locus_tag="IWO89_000411"
     CDS             complement(49339..50628)
                     /locus_tag="IWO89_000411"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HAQ0455197.1"
                     /translation="MTATFEKKGTNDGVLKFTIAQDEIQKGLTTAFNKVKKSLNVPGF
                     RKGKVSRQVFNRMYGEEALYEDALNAVLPEAYEAAVKEAGIDPVSQPKIDVESMEKGQ
                     DWVITAEVTVKPEVKLGQYKDLVVEKQDREVTDADVDARIKREQEAQAELVIKEDAAA
                     ENGDTVVIDFEGFVDGEAFDGGKGENYSLELGSGSFIPGFEDQLVGHKSGESLDVNVT
                     FPEDYQAEDLAGKEAVFKVTIHEVKAKEVPELDDEFAKDVDDEVSTLDELKEKYRKEL
                     VETKEKAADDAKDEAAIRMAVENAEIVELPHVMVHDEVHRSMDEFLNNMQRQGISPEM
                     YYQLTGSTEEDLHKQFEGEAEMRTRTNLVIEAIAAAENLQATEEEINAEIQELSETYN
                     MPIEQIKRVLTEDMLEHDVTMKKAIELITGTATEAKE"
     gene            complement(50782..51714)
                     /locus_tag="IWO89_000412"
     CDS             complement(50782..51714)
                     /locus_tag="IWO89_000412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0455198.1"
                     /translation="MKPIDFPEKYENLMRVAQQALANQQYQQAKELFQRAYELKESFE
                     ANSLLVFCLYELDEKKEALKQALLHEKQYLQNEEFAEFYFDLLISTQDFLYARKLIAS
                     TDFYEAFEQRVIEKIQFAEELSGQMERQKIKALHNKSEELPSLEPARQLASIDEIEQL
                     PYHEFIQTASKLIVSPEVHILARAKLLETLRQLNECHPVFYLTIEEKLVKVIPKDLPK
                     PQQQASYQQLCVFSDRYGNEDALLSSVLKEEFTLQSAIVYPVYDTYIEDPKRWFQLTV
                     EACTGKTMYEGTEDEKQDFLKKREKILQQMIFFH"
     gene            complement(51871..52530)
                     /locus_tag="IWO89_000413"
     CDS             complement(51871..52530)
                     /locus_tag="IWO89_000413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RDD family protein"
                     /protein_id="HAQ0455199.1"
                     /translation="MSELSNENKEQEKDKQQQPAVGPDSNFSQKVLSSFEREPLTPEE
                     IKKKQRKWKKYTDTRNEPPKANDFPKYFYAGFFIRMFAFLVDLLCIGAITRLTLGIAK
                     NLGWLNFSDSYLSLYGFTALLIYLAYFILLTKFNHGQTIGKMIFGIRVISFDEEELSW
                     STVLVREGACRFILKFPLLMLGYLPAVFNQKKQHLGDFFTNTSVVTINLIKAFNQEVN
                     S"
     gene            complement(52533..53558)
                     /gene="sppA"
                     /locus_tag="IWO89_000414"
     CDS             complement(52533..53558)
                     /gene="sppA"
                     /locus_tag="IWO89_000414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptide peptidase SppA"
                     /protein_id="HAQ0455200.1"
                     /translation="MNKRRWAAVLIAAGLFVVSLVSASLTKPQENDQIEGLNSWLYGD
                     EELAPNVLEEGDSTQKIVKLSVDGTIAAGGSSGLFASEGYNHKNFMEQLKAIEEDPTV
                     KGIFLEVNSPGGGVYESAEIAKKLDTIRKEHDIPMYVSMKNMAASGGYYISAQADKIF
                     ATEETVTGSIGVIMSGLNYSGLLEKLGVEDTTVKSGALKDMGSGTRPETKEEEAVLQA
                     YIDSAYSRFVAIVSEGRNKSEEEVKKIADGRIYDGVQAKEVGLVDELGFPEDALKAIR
                     KEQQLEDAELVEYSSTPSGFASTWLGSKLAEFQGLKPSETSQILSVLENLGTAESPKA
                     MYYYGGE"
     gene            complement(53571..53861)
                     /locus_tag="IWO89_000415"
     CDS             complement(53571..53861)
                     /locus_tag="IWO89_000415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0455201.1"
                     /translation="MDSKIEIMTLGMLKKQLSKFEASAGVSDDTKIFLDTGWDSIQEI
                     APDALEVAQAREFTVEDELTKESFSGYAREEKAERFDTSEQSETVIVIKNLY"
     gene            54270..56075
                     /gene="glmS"
                     /locus_tag="IWO89_000416"
     CDS             54270..56075
                     /gene="glmS"
                     /locus_tag="IWO89_000416"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HAQ0455202.1"
                     /translation="MCGIVGMIGLKNVTPGLIDGLEKLEYRGYDSAGIFVSDGTTDYL
                     VKAQGRIQNLKNKINIDTTGNIGIGHTRWATHGQPSEENAHPHTSQSGRFVLVHNGVI
                     ENFEELKIAYLANDHFIGETDTEIIAHLIETFAKDTTTQEAFLKALRVIKGSYAFALI
                     DRTAPDVIYVAKNKSPLLIGLGDGFNVIASDAMAMLAHTKEFVEIEDEEMVTVTSEKV
                     IIQNFAGDIVERSSFEAQVDASDIEKGTYPFYMLKEIDEQPIIMRRIAQKYVTEDNRV
                     SLDQKLVDTLSDSDRIYIVACGTSYHAGLAGKQTLEKLTQIPVEVHLASEFGYNTPLL
                     SKKPFFIFLSQSGETADSRQVLVKINRLGYPSLTITNVAGSTLSREASFTLLLHAGPE
                     IAVASTKAYTAQIAVLTLLAKAIGDKKEFETSLVFDITHELSLVANAMESVIAQKDYL
                     EELAEVYLSDTRNAFYIGRGADYNVSLEAALKLKEISYIQAEGFAAGELKHGTIALIE
                     EGTPVIGIISEEVTGAHTRGNLKEVESRGAKTLVIVSEGLEKESDQLVLPTVHPYLTT
                     LTTVVPTQLLAYYATLLRGFDVDKPRNLAKSVTVE"
     gene            complement(56147..57502)
                     /locus_tag="IWO89_000417"
     CDS             complement(56147..57502)
                     /locus_tag="IWO89_000417"
                     /EC_number="5.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HAQ0455203.1"
                     /translation="MGKYFGTDGVRGEANKELTPELAFKLGRCGGYVLSQHEEEGKRP
                     RVLVGRDTRISGQMLEEALISGLLSVGIEVFQLGVISTPGVAYLTRLQKASAGVMISA
                     SHNPAQDNGIKFFGADGFKLVDDQELEIEALLDAEVDELPRPSAEGLGTVEEFPEGLL
                     KYSQFLVQTINGDLSGLTICVDAANGATSTSVNRLFADLETDFYTMGTNPDGLNINDG
                     VGSTHPERLAEMVVEKGADAGLAFDGDGDRIIAVDELGNIVDGDKIMFICAKYLAEKN
                     RLKKDTIVTTVMSNLGFHKAVEGIGLKDVVTQVGDRYVVEEMRKNDYNFGGEQSGHMI
                     FLDFNTTGDGMLSGIQLLNIMKQTGKKLSELAAEVTIYPQKLVNIRVSDKNGAMDVPA
                     IKKVIEEAEAEMGSEGRILVRPSGTEPLLRVMAEAPTDEKVNYYVDKIAAVVKEEIGL
                     D"
     gene            complement(57526..58695)
                     /locus_tag="IWO89_000418"
     CDS             complement(57526..58695)
                     /locus_tag="IWO89_000418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0455204.1"
                     /translation="MISKEKRTSILYSFIALLFSIALYFNANGQSVPNTLSGNEAYNQ
                     TVTGVPIKISYDSKRYYIHGYENAVTVKLSSANRVQLNAEANEDTRMFRVTADITKLG
                     EGTHEVPLKIQNLSSAVTAKIEPSTITVTVEKKVIKDFDVETLIPSTFTPSGYELDDT
                     SVSPKKVSITTGDKTLAEIKRVVADVDASMVTDDGINSEVPIQALNAAGEVLSIVSDP
                     VQVTVRADSIKPSKSVRLYGIQQGTPAAGVESYDFSFSKLEAEVSGSSDLLASIGDSI
                     AVPINVSGINHRTTRKIEIPVEEGLSIKPESVSVEITPVLQTSSSSSASTSSRTQENT
                     STSRSISSADSGTEPSTTTISSSSESGTSSSTTSTEETSETSESTQMSRTNSSEN"
     gene            complement(58692..59579)
                     /locus_tag="IWO89_000419"
     CDS             complement(58692..59579)
                     /locus_tag="IWO89_000419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00159 family protein"
                     /protein_id="HAQ0455205.1"
                     /translation="MSFQIGELFNLDYWQQMFSGDLFSLNFLVNILDILVVWYLVYKL
                     IQLLRGTKAIQLLKGVAIFIVIRIVAEIIGLHTLSWLMNQVITYGVIAAIVIFQPEIR
                     RGLEHLGRSSLFKQSKSEQQEDEKMILSFDKAIQYMSKRKIGALITIERHTGLDEYIE
                     TGIALDADITGELLINIFIPNTPLHDGAVIVKEGKIAVASAYLPLSESMLIPKEFGTR
                     HRAAVGISEVSDAITIVVSEETGDVSITLDNELMAGLSQQEYLAILRRELIAEEKTKN
                     KKNVIQSFLDGMTKGGDRK"
     gene            complement(59768..63460)
                     /gene="nifJ"
                     /locus_tag="IWO89_000420"
     CDS             complement(59768..63460)
                     /gene="nifJ"
                     /locus_tag="IWO89_000420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate:ferredoxin (flavodoxin) oxidoreductase"
                     /protein_id="HAQ0455206.1"
                     /translation="MRKMKTMDGNTAAAYISYAFTEIAAIYPITPSSTMAELVDQWSA
                     AGKENVFGQPVKVVEMQSEAGAAGVVHGSLKTGALTTTYTASQGLLLMIPNMYKIAGE
                     LLPSVFHVASRAVTTNALNIFGDHGDVMAARQTGFAMLAESSVQEVMDLAPVAHLASI
                     EGEVPFLNFFDGFRTSHEIQKIEVLEYEELAPLINQEKLASFRKRGMNPNHPTVSGTN
                     QNPDIHFQQRETINPYYDKLPEIVQKYMKEINRLRGTSYDLVTYYGADDAEEVIVSMG
                     SVAQTIEQTIDYLNANGRKTGFLNIHLYRPFPVETFVQKLPHTVKTIAVLDRSKEPGA
                     GGEPLLLDVQSALYDSELRPAVIGGRYGLGSKDVTPDQISAVFDELKKDPSIRKKRFT
                     IGIVDDVTYQSLEPKESLDLTEPQTFQAKFWGFGSDGTVGANKSAIKIIGDHTDKYAQ
                     GYFYYDSKKSGGLTVSHLRFGDTPIRSAYLVEHADLVACHTPAYLHSYDLVKGLKPGG
                     IFLLNTLWSDEQLETHLPLKLKRYLAENNIRFYTINAMRLAQEVGLGRRINTAMETAF
                     FKLADIIPFDEVLPLLKEEALKSYGHKSMAIVEKNIQAIDRTTELLHQVAIPDHWRTL
                     EMPEKINKRNTGYIQEILEPVNAQEGNQLSVGTLVANGMTTGEMPLGTASMEKRGVAL
                     EVPEWISDRCTMCNECAFVCPHAAIRPFLADEEEMEEAPEGFIVREMRGADGVKYRIQ
                     VSVEDCTGCGLCVEACPAKEKALVMKPYEEQKEQAVNWAFAMTLRQKANPVKGKNTVL
                     STQFNQPLLEFSGACSGCGETPYVKLLTQMFGDRMMIANATGCSSIWGGASPASPYTT
                     NECGQGPAWSNSLLEDNAEFGYGMLLAAQTRREALASSIKEVLPKVSEELRELMEDWL
                     VHMFESEGTQQRAAKLQAAIESERSIFPELEDIYKRKDLFVKNSQWMIGGDGWAYDIG
                     FGGIDHVLASGADVNILVLDNEVYSNTGGQTSKATPASAIAKFSASGKYASKKDLGMM
                     AMTYGNVYVAQIASGANQMQTIKAFEEAEKYPGPSLIIAYTPCITHGLLGGMKESLKE
                     AKEAVSSGYWSLYRYNPTMVEKNKNPMTLDYKKPDFSAMPDFMRKQVRFSSLDNANPN
                     LAKALFEKTVHDAKTRFYNYAALTGELEKIKKRLEPEESIDVPVKTRKEKTTDLEAEE
                     RRAARRAARAERRKKMEQATKEKKEK"
     gene            63659..64180
                     /locus_tag="IWO89_000421"
     CDS             63659..64180
                     /locus_tag="IWO89_000421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsbA family protein"
                     /protein_id="HAQ0455207.1"
                     /translation="MDISVIDATKTNTQKGILYGSSNAPKKMVEFINLACPYCRQWFE
                     ESYELLEEAVQSGQLQRVIKLFDKEKESLQRGNVMHRYLTISDGQKAIKEIKQIFDTQ
                     DEWKHLSLQEVADFAVDKLKLTEQKDEQLSQAVINEAEQAHIRFVPTVILGKEIFDES
                     ISIEELKELIQTK"
     gene            complement(64879..65436)
                     /locus_tag="IWO89_000422"
     CDS             complement(64879..65436)
                     /locus_tag="IWO89_000422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0455208.1"
                     /translation="MRNLSNRNKILIIIVVIAVFHLGTNAVLSRIILGPKPPRPEITR
                     GEFDFRLEYEVDGERIVIEDTIVALFDGFSADAGSMAWYRTWRLHLASDRRSRNILLD
                     ELEDGRRIYYVPESANYSMGDVQKKREPNPNWYPFNGVTIEYPRNKTPEIGAKFISGL
                     EDLYDRFGIRLISWEHDPPIENRFE"
     gene            complement(65615..66226)
                     /locus_tag="IWO89_000423"
     CDS             complement(65615..66226)
                     /locus_tag="IWO89_000423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0455209.1"
                     /translation="MNSNRGNKMEYNGSMRIVQIIFWITSGIVIIGGVFLMLPSLIFP
                     FFALISPKIPEPEITYGEFPFRIEYELDGQINIIEDTVIAEFNGCEFSAGSMKRERRW
                     RSRLASGREDLPFGDDLGIYFSRGSAQYYMGENVQSMSLKPHIALRNLEEGFKREVDF
                     ILGESGYIRTVLNFGEAQEVLTQYGITLINWEISEPIVNNFGD"
     gene            complement(66339..66596)
                     /locus_tag="IWO89_000424"
     CDS             complement(66339..66596)
                     /locus_tag="IWO89_000424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0455210.1"
                     /translation="MRAVHVNPTIEITMIGHSKGGAEAANSVAINTNAIIFNPATVNL
                     SAYELDSNTYTANMVAYVVEGDALNVVFGGVYTYWRINHVR"
     gene            complement(67193..67471)
                     /locus_tag="IWO89_000425"
     CDS             complement(67193..67471)
                     /locus_tag="IWO89_000425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0455211.1"
                     /translation="MEKHNITGSMYVDDQWFISTDEVKIAAIRRDYLQKIHKIKDPNG
                     KKARLIITLITSNGKKLKLAGSYDNVKKFEHWYLQKDSISLNKQFEKN"
     gene            complement(67505..67756)
                     /locus_tag="IWO89_000426"
     CDS             complement(67505..67756)
                     /locus_tag="IWO89_000426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0455212.1"
                     /translation="MKEKPINKWSLYKNSMFGVVIFGVCAGLFLFILLQTVTLFSSNP
                     TYGWRDSIALGMFAGGYGFLLLFSILTLSPIHGLIQLWK"
BASE COUNT        19064 a        14750 c        12002 g        22413 t
ORIGIN      
        1 caacctctta ttcacggtgt ttcagaagta gcttctcgga aataagccga aatttcacaa
       61 aaatttgaga aactattttt aggaaacttc ttcttatttc ttaaagctga ccacttctat
      121 cacaacctct ttttgactat gcattcacat cgatcacttt agaatcatca ttgatgacat
      181 cgcgatctaa atctttgatg cgtgcacttg tcacgatacc agctaccatg ctaccgctga
      241 cgtttactgc tgtacgtgcc atatcgatta atggttcaac agagataact aacccaacga
      301 ttgctactgg caagttcatt gcccctaaaa cgatcagtga agcaaatgtt gcgcccccac
      361 cgactccagc tacaccaaaa gaactgattg ttacaattgc cacaagcatc aaaataaatt
      421 ccaaactaaa gacattcact ccagctgctg gtgcgacgat cgttgccaac atagctggat
      481 aaacgccagc acagccgttt tgtccaatag acaaaccaaa acttgctgag aaattagctg
      541 ttgcatcatc tactcctaaa gcttttgtct gagtctcgat attcaatggc aaagctccag
      601 cacttgaacg tgaagtaaac gcaaagctca ataccggaaa ggcttttttg aaatagttga
      661 ttggattgac ttttgcacca atcaaaatca gtgagtggac aatcaaaaca gcaagcaatg
      721 ctacgtaaga agcaacgaca aatttaccta aattgacgat agctgcaaaa tcacttgttg
      781 ccaaaacgtt tgtcatcaaa gcaaatactc cataaggtgt caaacgcaaa acaagcgtca
      841 cgatccgcat aatgatttta tacagactgt cgactaaatc tgaaaagaat gctgcttctt
      901 ttggcgcttt tcttttcacg cctagataag cgattccgac aaatgccgcg aaaataacga
      961 ctccaatcgt acttgttgca cgactacctg ctaaatcagc aaaaacgttt ttagggataa
     1021 aggataaaat ctgctgtgga atcgataagt tttcaactgt ttcctgacgt gttgccagtt
     1081 cagagattcg tgctgtttct gcagctcctt gtgtgaattt agctccttgg agtccgaaca
     1141 gcattgttga accaattccg atcaatgctg caatagctgt tgttccaagt aaagaagtca
     1201 atacagtcgc actaattttc cctagctttt cagaaacttt catttttgtg aatgcaccaa
     1261 tgatcgaaat aaacactaaa ggcataacta gcatttgcaa gaatgataca tatccttgac
     1321 ctacaatccc gatccattcc attgattgag taacgacttt gtcgtctaca ccaaagatca
     1381 attgaagcag accgccaaag acgataccaa ctcctaatgc tgtaaagacg cgtgttgaaa
     1441 attttgtatg gcgcttttgc atacgataga acacatataa taatgccgca aaaactagaa
     1501 ccactaaaac tgtgatgagt gttgtcattt ttctcctcct catattatta atgacaagtc
     1561 catcgaagga agtaaaggtt cccgagaatg attcttcaac catcctcctg aaaaaaattt
     1621 tttcagtgac ttttatattt tagacgattt ttatcgaaat gacattcttt ttgcttacaa
     1681 ttagtaataa aaaaacatac ctgtgatagc tttaatctat cgcaggtatg tttttactgt
     1741 ctattcttgt ttttctgatt gaaaattcaa ttttacccca ggctgatcta aggcgatctc
     1801 tgtcacctca taagacattc ggcgtacgat atcgaataac ggtgctacta gctcgtccac
     1861 ttcttcttgt gtgacatcat tttgaccttc aattttccga ttgaggatat gattgatctg
     1921 actcacactt ccagataaaa catatgtatc aaacactaaa cggaactcta agcgtgctcc
     1981 tacgatgcta tgttctttcg gatagttttc gatcgtttct tttaaaggcg agaagcctac
     2041 tttcaaatct gtttctactt cttggttcgg caatctcata tcataatgaa atgcttctac
     2101 aacttctttt tgtcttctga tttccatata attttatcac tcctttatga aaattataac
     2161 atatctcctt tgaaagggga tactactttt taatgatgct ctgcaaggta aggatttcat
     2221 tcagatttcc taccacataa tctgccggga tctcacccag aaaatgatcg atgtcgtaaa
     2281 gacaagtagc gactcctgca tttttccctg cttcgatatc taaacgacga tcgccgatca
     2341 tcatcgtgtc tgttctttct aaatgaaaag tatcgatcaa ataatttaaa gatgctggat
     2401 caggtttacg aggaaagtcc tgatcgatcc ctactacttc ctcaatcaga tgtgccaatc
     2461 gatgttcttt taacaggccc catgttgatt ctgtcaaacg gtgggtcagg atgaaatgac
     2521 gaccgccgtt gtcttttagc atttccagta cttgtttcgt ttctggaaat ggacgggaaa
     2581 cttccgtact ttctttttca tatcggtgaa ataattcatt aaactctctg gcattcaagt
     2641 ttgattcatt gatcaaataa ctagtagaat actttttcat tttgaagtag atttcttttt
     2701 tgtccatact gataccaaaa tcttttagtg cttgccatgc accatctacc attgcgggat
     2761 acgtatcaaa caatgtacca tcaaagtccc atatataatt tttcatgttc cttttgttcc
     2821 attcctttgc ttcttttttc tatctctcac ttttagataa aggtgttctt taaagtattt
     2881 ttttgtccag aattgtcaaa tcatctgcta atgtatactg gattggttgt gcatttggga
     2941 tatcgatcgt atccatttga tcttcaggaa catcttctaa atattttgtc agtgctcgga
     3001 tgctattacc atgacccaca actagtaaat cttgccgatc ttttagtttt ggtgctagat
     3061 gatcttgcca taagggaatg atccgattca ctgtcatttc cagactttct ccgtatggga
     3121 tcaaatgcgg atccaagtga tcatatctgc ggtcaaatct attttctgcg gttaacggcg
     3181 gaacttccgt atatcctctg cgccatgttt ttacttgatc ttttccgtat atttctttca
     3241 tcttatcttt gtttttaccc acaagatccc cataatgccg ttcatttaga cgccatgttt
     3301 ttattatagg aagccataac tgacccattt cttctagtgc gacttgtccg gtaagaatcg
     3361 cacgtttcaa aacagaggta taaacggcat cgaattgaag tccggcttca agcatttttt
     3421 gaccggcaac tgctgcttgt tcttttcctt tttctgttaa ggcgacatct agccatcctg
     3481 tccagtaatt ttcaaaattt gcttcgcttt cgccatgacg taataatact aatttcattc
     3541 ttttctccct taaacattcg tttcgaattt tataccacat gttttgcatg tcacacgtat
     3601 tcgtcctttt cctttaggtg cacgctgctt ttgatgacaa ttcgggcact caaaaaaagt
     3661 ataacttttt cgatccattt tgacttgctt tttgtttttt atctgatgat atttttcttt
     3721 gactctatca agatatttca aataacgttg attctcattt agtctggggt agattttttt
     3781 tgataggaaa cgaaatatca tccatccgaa aaagacgatg aacaacaatt gtccgattct
     3841 atagggcaac caacgactta gcaaactaaa gacaagcgca gcgatcaata gaccgcgatt
     3901 caactgatca aatttcgcat atcttccctg cataagtttg tgatagttct gctgaaatcg
     3961 tattagcctt tctaaccatg tttgcatcat attatctcct ccatcatcta atatttatta
     4021 tgacaaaaaa atccaaaaag aaaaagcctt aattggcctt ttccattgca acttggtgaa
     4081 ggacattccg atttctggta aagtcaatca caatcacttt ctttttatct tctctgttga
     4141 ctactcgaaa tgtgtccgtc aatggaagaa acagcttctt agatggtagc tgttgcatac
     4201 gtataaaacg atggatcatc gtaatcaatt gaggaaattg aatagacgtg tgaagcagac
     4261 ctggatattt tttcaacagc tgtgtcgttt tgatcagatt ccgttttttt actgatcact
     4321 tttaaaaagc tttttagaat cttttgctga cgtctaaaaa tagaaaaaga attttcgtca
     4381 tctatttgat aacgcatttg tttttgcagt tctgctgcag tcagatggat cttcccatcg
     4441 tttgtatcga tcattaaacc ttctggaaac agttcaacca ctgctcgatc ccaagattca
     4501 agagaaagct cgatataatc ttttactggt aattccaatt ctaccttaaa aaagtgcaaa
     4561 acagccgaca gcccttctgc ttgatagatt tctcctaatg ttagcccctc tagatggaac
     4621 tctggtgaaa aatgcagcaa tgttttattt tcctgcaatt gaacatatag aaatgtagat
     4681 cgctcaatca gagtatcttt taaaacaact aaaaaattca tttttctccc ccttttttca
     4741 tcatttttaa aatagcatat tttgaaaaga aagtctgatg cttagagtag aatttcataa
     4801 aaatttacta tttcataagt attcacgaga aaaatacaaa caaaaaagcg gaaataacgc
     4861 tcatccgcct tcttgtttgt gtttatttcc tcttcaaacg cttgtctatg attcagactt
     4921 ttctctatcg ttattttcat ctaaaagttc ttgcattccg ataatttctc gatattgatg
     4981 aatcttagtg ataccaatga ctttgtctac atcaatacta aaacaaatac cattacctgg
     5041 aatatcgatg ttgatgcctt gaccaatcgc atccattact ttattcgtca aactatcggg
     5101 tactaatgtc aacacaatat ctttttctgg cgaaatgtcc aaacctaaaa atttccctga
     5161 atcatgtaca ccagaacctc ttccatgtaa gatcgttccg cctgctgcac ctgcttcttt
     5221 tgaaaagtcg ataacatctt cgcccattcc tccgtcaacg atcgtcacaa tcatgtgaag
     5281 attcaatgct acaagatctt tcatttgctt tcccccaatt tttgcaatct ttctttttcg
     5341 attctgtctt tttcatcttt tcgtttataa atatatccta gtactaaagt ggataatatt
     5401 ggtgtcaatg caatcattgc aaccacacca aacccgtcaa tgatcggatc tcttccttca
     5461 atttgtgcag cgattgccac gctcattgat aaaataaatg tcgaacacat cgggcccgtt
     5521 gctactccac cgttatcaaa agccattgct aaaaagatct tgtccacttt tcgtcctaaa
     5581 ataaatgcta gcccgtatcc tgggatcaag aaataataaa gagagaatcc gacgagcaaa
     5641 cgccacatag acaatcctac tgctgcgccg atcccaattg aaattaccat cagcatgatt
     5701 ttcgatcgga ttctcccctc gctcaattct tcaacctgtt tgatcattac atgaatcgcc
     5761 ggctcagcaa accctactaa gaaccccatt aaaaagccaa ggggaaccag tatataattg
     5821 taaggcaatc ccgcaatagc actgcctaaa tgctgtccta ccggtacata agccatattg
     5881 actccgtgca ggaaaaggat caacccaata gtcgttatca agaaaccttt cataatgtca
     5941 aagaaacgct ttttgcgcat acgaaaacta atgatattca ttaaaacaaa taggattacg
     6001 ataggtgcca ctgccaaaat gacttccatg gtaacgattt cgattccatc tatcacttgc
     6061 tgccagaaac tcattggaag atcaccccca aaatcatcac ggcaagaatc ggccctaacg
     6121 aagcaatacc gaccattcca aaactgtcat tttcattttt cttttgtttg atcatactag
     6181 tcatccctcc ggcaagggcc aagataaatg gaacagttac tggaccagta gtcactcccc
     6241 ccgaatcaaa agcaatcggc ataaattcgg aactggtgaa aaatgaggcg atcaaaacgc
     6301 ctatgtagcc tatagccatc aattgataat aggatagctt gaatacgacc cgaagcagcg
     6361 caaaggctag aaatactcca gtgccgacgc tgacgatccc aatcagcaac actctgccaa
     6421 tctctccgtc agatatatca tatacttgtt gtcccaaaac ttgcacggaa ggttcagcaa
     6481 tcgtgatcac gatcccgata gcaaatccca aactgaccaa aacggtcaaa cttttctttt
     6541 tgatcatata tttccctact agatctccga cttccatcat agaataatct gctccaaaca
     6601 aaaagagcgt cattccgatc atcatgattg ccgcaccaac cagaaatgaa gcgaggtctt
     6661 ctccttctaa cggtgcaaaa acaaacgtta aaatgacaat caggacagtc atcggcaata
     6721 tagcagcaat cacttcttta aaatttttta gcatgtatcc tcctccttta ctctttaagt
     6781 ttaccttaaa atgaaagagt tttcgagcac aaacttaaaa cgttatcatt taatttttct
     6841 caacataaaa taacacacgt cccaaaagta tctttttact tggaaaaatg actttgaagg
     6901 aacgcgctta ttgtgttttt ttattttaca gagccgatca ttccttgaac gaattgcttt
     6961 tgcaaaacaa aaaatacgat ggcagttggt aaagtagcga tgactaatcc agccatgatc
     7021 attccatagt ctggtgtata ggaagcaccc atagcagata aaacaagcgg aaccgtccgt
     7081 ttatcaggag attgcaaagc tactaagggc cataaataat tgttccagct actcatgaat
     7141 gtgatgattg cagccgcggc ataggtattt ttagccgtcg gcatatacac tctaaaaaaa
     7201 atacttagtt cttttagacc gtctaatcga gcggcttcta ctagatcttt tggaaaagat
     7261 ttaacatttt gtcgaaagaa gaagatcaaa aaagcagtgc taacagatgg caggatgaca
     7321 accaagaaac tgttgattcc caaaaccgtt ccgttcaatt gtgaaaacat tcgatataaa
     7381 gggatcatca atgccgcaaa aggaaccatc atcgatagca gtagaatatt gaaaattagg
     7441 tcttttcgtt tactttgata aatctcaaat ccatatcctg ccattgacga aatcagcaat
     7501 gcaaacacag tggtcaccaa agcgatcatc gctgaattcc aaagggaacg agtaaaattc
     7561 agctcattgg aaaacaaatt ttgaaaattg atgatcaagt tactgccaat ctttattttt
     7621 cccgatgtga tatccacggg tgtattcgtc attcccaaaa ccatccagac aaaagggaaa
     7681 atggaaataa atgataggat gcttaatgct gtatacttta atactgaccc aattgtcgaa
     7741 cgaagttcta atgcttgttt tttgacgttg gggcgttcga ttgtttcttt ttctaccata
     7801 tttttcatcg actttctcct cctactttcg tttggattat cgacaatatc acaataaagg
     7861 aaacaataac aaaggaaact gctgccgcat atccaaaatt aggcgtgaat ttataactca
     7921 aattatagat gtattgagat agggtggtcg ttgcatttgc tggtcccccg ccagtaatat
     7981 tttgtacttc atcaaaaagc tgcaatgtcc cgatcgtcga agtaatggaa gtaaacaaaa
     8041 tgattggttt taaatttggg atcgtcactt tgataaactg ctgccattta gaagcaccgt
     8101 caatagatgc tgcttcataa atctccgaat caatattttg catgcctgaa aggaaaaaaa
     8161 tcatattgta tccagtccaa cgccaagtga tcgaaagaat gatcagaact ctcgcccaaa
     8221 ttggatgagt caaccagcca atcggtgtgt caatcatacc aatgcccaac aacaagttgt
     8281 ttacaaggcc agattgagaa aatagacttt tcatgatcag agaataactg accatcgaag
     8341 tgatacatgg aagaaagata gcggttcgaa acaacccttt atatttcaac tttttatcat
     8401 ttaaaaggtt ggaaataaaa agtgctagaa acaacatgat cggtacttgt acaagtaaat
     8461 acaggaatgt attaaaaaaa gcttttttaa aagttgcatc tgtcaacatc cgctgataat
     8521 tacctaaccc gttaaatgtc atattgacag ggggaccact ttgaaaagac gtgatcaaag
     8581 ccaaaaccat cggaataaaa accatgattg atatcaaaat gactggcaag attaaaaaag
     8641 catgcccgtt tttgaataaa tctttctgct tcattctttt ccctccaatt ctatcattga
     8701 ttatctacat gaaaagacaa gaaagacaat atctgtaagg cagacgtgat cttttttgtc
     8761 ttttcgtttg tctgatgaaa aagcgaagaa attcactttt ttaccaccga tcaattagcc
     8821 aattgtgctt ctacttgttt ttgtgtatct gctagtacct tgtctgtctc ttctccattg
     8881 atgattcgat gaagagcttc agctacgact gcttcaattg cataggtctc atgtccgtaa
     8941 ttcacttctg gtatttcaga tgtccatgtt gaaaaatcgt caaagatctt ttgaccactg
     9001 taaaattcag aagcttcttg ataagcttct tgttcattcg cgctaagcat cgtagatact
     9061 agtccgattt ctttcgcaag agtgcccatc agttcttcat tcgaagcaaa cgttgcttct
     9121 aggaaatctt tcgctgaatc ttctccagct actcctttga tcacatacca gccagcgccg
     9181 ccaaggttag aagcttgcgc tttttgcaga ttttctggaa gtgcaggtat tggtgcaatc
     9241 ttccattttc cagattggtt ttctgctcct tggatcgttg aagaatacca agcgccagtt
     9301 gggctagaag ctactgctcc tgattgtatc gcagttactc cagcattcca gtcagaggtt
     9361 tgttcgacta gatcttcttt caaaagagtg gcaaacaatt caagaccata ctttaaagat
     9421 gcattgtctt ggatcgtgac tgtctcccca tcttcagatg tataccattc tcctgcctgt
     9481 tgcataatca tccttactcg accaaggtca gaaggattga cttcagcgat tttctttccc
     9541 gtcttttctt tgacatctcg cgctacttga atatactcgt cccacttcag attctccata
     9601 tcctcttctg tataacctgc ttctgccatc agatccgtac gatagaagtt tgctgttacg
     9661 cccgaatcga aaggaacacc gtatatttta tctcctactt tgtttaccgc aaatttatac
     9721 ggtgcaaaat gatcttcctt tacaatagca gacaaattag cgaatgcatc tggataagaa
     9781 gtcaaatagc cttgtatcct ataatcttca atcaagacga tattgggcaa tccttctgta
     9841 ttcccgctcg ctaaagcggt gttcagtttc tgaacgatat catcctgaga catcgtaaca
     9901 acttctatct ctgtttcttc gttctcataa actttttttg cttcattcac tgctttgata
     9961 ttgaatgtct catcccacgc ccagacagtg actttttctt ctttgtctgc tgtactacta
    10021 gcacttccgc atcctgttaa gaaacctgat gctgccgcta aacaaacgac tccagcaatt
    10081 aactttctga tgttcatttt tatccgctcc ttttccatcc attagttgtt tacgtttgct
    10141 acattcatca taccaaaatt tgaaaacggt ttctttatta ttcagatact tcaaaaattt
    10201 ctcctaaaat aataaaaaaa tacaaatcaa ctatgacaaa aggaataatc ttagcatcta
    10261 gaccaaagat cgaaatggtg aggtttcttt aaaataaaaa tatccttgca ttttctccta
    10321 gaaataagta tttttaaatt acaagactat tcttaaggcg ggtaaaaaaa tgccaatacg
    10381 cgtaattctt atcgatgatg aacagacgat tttagatgga atgaaacagt tgatcaattg
    10441 gtctgagcac gggtttcaat taatagggac tttttccagt ccgctgaaag cgcttgattt
    10501 ttctttggta catgcaatcg atatcgtggt cacagatgta atgatgcctg aattaaatgg
    10561 aatcgaatta tcgcggctat taaaacagca acaacctgaa acacagatcc ttatcctctc
    10621 tagctatgat gaatttgata tggttaaaga ttcttttaaa gaaggcgtcg ctgattatct
    10681 tttaaagcct cgattgagtc ctgatttttt tctaaattcc ttaactgccg tttcacaaaa
    10741 aatcaaaaaa aagcccaaca aaagcccaat caccaatcgt tatgagcaga tctgcgagaa
    10801 tctttcacag cagattgctg gacaaactcg tccattgctt gaccctcttc tttttgaaca
    10861 caccttgttt ttctgttata taccgaaaaa cggccattca gttcaaaaca aggctctaaa
    10921 caaaaacagt cgatcaccca ttcattgatg gatagcgacg attcgtttac tatttatcct
    10981 gtttctacct tttcggaaga atccggctat gtaatcaata ctgattccaa agatacactt
    11041 aggaagtttg ttcaaaagtt tcgtcaagaa gtttttccag gtgatttttt tgttgtttct
    11101 gattcttttg caatccaaga tatcaatgaa atattccaag agatcagaaa ttgcaccaaa
    11161 ggacaacgtt tctatcataa gggaaagaac cttgcttatc agcatgaact tcttccgtta
    11221 ttaaccaacc acgagcagca agcaagtagt tatttaacaa aaatcgttca taaagactac
    11281 gctttagctg tcaaagaact tgagaatttc ttcacgacga tattggaaca gcctattgat
    11341 ccagcttatt tgaaacatga ggcaatcaat cgtttctatg cattactcaa cgctttggct
    11401 gacgagcatg ttgggaatga agaactttct ttgctcaaga tcgcccttcc ttctttatta
    11461 gccgatgctg agtatttcga agatttttct attcttgtca aagaaacact tgagaaactc
    11521 acccactttt ctgcaaatgc aaaaaaagac aatttcgata tcttgacttt gatctacgag
    11581 ttcgtccagg aaaattatga gaaagaaatc aatttacaac ttttatccga aaaatatcat
    11641 ttttcatata gctatctctc agccatcttt actgaaaaat ttggtatcaa cttttcaaaa
    11701 tatctaaaaa aagtccgtat cagtaaagcc aagctctttt tgacggaaac acggcttact
    11761 ttgacggaaa tctgtgagaa agtcggctat accgaactgg gttatttctc cagagttttt
    11821 aaagaagaga caggaatgac accttctcaa taccgtaaag gaacttttgt aaaatgagaa
    11881 aaaaaatcaa agagcatgaa ctctttgcca aacttctgtt cattttgttc atcggtcttt
    11941 ttctgcaagc catcgtcatt tctttgttta tttatcatcg atcaagggat gcctatatcc
    12001 agctgttcaa tcaatcgaat gatgtggtac taaaaaagat tcaatctgaa tttgaaagtt
    12061 tgaatgatac aatcgagaat acattagccg cttttgattc taatccagct gtcaaaagct
    12121 atttttccaa tgatccagcc caacatatgg aacagcttca gcaactaaga acaatccaaa
    12181 aaatgaatga ctctctttca aaaatccatc caatgatcga ttacgatgta ttgatctttg
    12241 gagaaaatgg aagaactttt gtcggaaacg acatgctcac tgccgtatct gccgattctt
    12301 tttttcaatc tgcgattgct cagagagtga atgaacgtgc tgctgataca caaatgcttt
    12361 ttgcacacca cggattgact cttcgcgata agaaagcacc cagcgtcttc tttgtcaaaa
    12421 aactgaaaaa tacattgaat cacgtttacg gatatgctgt tttatcgatc tcatccaagc
    12481 aactagcgaa tctttttcaa tctgtagtaa atcctgagat atcgaagatc tccttcatga
    12541 acgaagaaca gacgatcatc gcatcaaatg aagaaaatac aattgggagc cgatcaatga
    12601 ttttcaaaaa actaacagta ggtgaaacaa cgatttcaaa tggacagcag ttgacccgtc
    12661 ttcctctgta ccgccaggat tgtacattac tcagtcaagt cgatcttcat tctttatcta
    12721 accaaatggg cgtgattttt ccgattattt tgtataatat cgtgacactt gcttttgctg
    12781 gctcattggt atttatgtat ttggatcgac acacaaaatc gatctaccgc ttgattgatt
    12841 cgctaaaacg gctagaggaa gatggcttaa aagctactgt tcctgtacaa ggaagccatg
    12901 aagtccaatt gctagggaat acgatcaatc aattgctaag taatgctcag catcattatg
    12961 aacagacgat ccaagacgaa aagaagaaac gttcgcttga aatccaaact atgcaagcac
    13021 agatacagcc ccattttatt tataatacgc tgactagctt gaaatttttg atttggcaga
    13081 aaaaaacaga tgaagcagtt caaggaatcg acagtttcat tactttacta agaagtacga
    13141 ttgggaaaaa agaagaagtg attccagtca aggaagaaat caaaagcgta caaagttaca
    13201 tcgatctttt gagtatgcga tatggcgaaa gaatctcggc taaaataatg attcctgatg
    13261 aactgcagtc attatctatg ccgaatatga tccttcaacc aattatcgaa aacgcctatc
    13321 ttcatgcttt tcacaataaa acgcaaggat tcatcacggt gtacggcaaa acaaaaaatc
    13381 agcttctttt attcgaagtg attgataacg gcgatggctt cgatactgaa aataccaaac
    13441 aaaaaaatga tcatttttca ggaatcggca tcagccatgt agatgagcgg atacgcctaa
    13501 tgtatggcga tgcatatggc ttaacgatcc aatcagtgat tgggacagga acgactgtaa
    13561 cgatttgtct accgcttctt cctaaaaatc aatcagaata tcaaaaataa agaatagcca
    13621 agcgctgatt tttttatttt aagttaaata aatcaatcag tattcttgac tttcatctac
    13681 gcgtctcttc tactgtcagc actggataac agcccaatat ttgactttct cctccgagca
    13741 tcttgatttc ttggagcgca ttctctacta atgtcattgg acggtccaac aacaaatcga
    13801 tcacgaaaaa atattctccc aagctagttt tcaatggtcg ggattcaatc ttacttaagt
    13861 tgatttcccg ccagccaaaa gctgccagca ttttatgaag catccctggc cgattagcag
    13921 gtagtgtcaa gatgacactc atttttactg gagctgactg ctggctggtc atctttcggt
    13981 caccgacgat ccaaaaacgt gtctggttca actcattatc ttggatattc tcagctaaga
    14041 tctccaatcc aacatgctgt gctgtttcta atgaagcaat cgctgctgcg tcttctttcg
    14101 gatgttctgc tacatacatt gctgcagcag ttgttgattc tgttgctact aaaggaacat
    14161 taggataatg cgtttctaaa aactgttgtg attgtgctag tgcttgtgga tgtgaaagga
    14221 tttttgtaat ctttgttgct ggattgccta aaagctgctg cttgatagga agaacgattt
    14281 cacttttaac ttcgacttct ggatgtttgg aaagaagatc gattgtgtga tgaacagctc
    14341 cttccaatga attctctaca ggaacgacag ccagatcgac ttgtttgcga aacaaagcgc
    14401 gcaaacaaac agggatggaa gcatacgcag tcaattgttc tgtaggaaat agctgacaag
    14461 ccgcctgata agtgaatgaa ctttctggcc ctagatagct gacgttcata tgcttttgat
    14521 cctttctata atggtatcga tgatcttttc tggtgtttga ttggtcgtgt tgattcgatg
    14581 tgtcgcactt tcttcatacc aagaaagtcg ttgtgccaat aatgctttta tttcttctct
    14641 tgtttttcca agagctaaag gtcgaataga cgtttcatct tgttggatac gatcaactag
    14701 acagtccgct tctgcctcta agtaaagaac aatcggctgt gtttttaaaa aacgccgatt
    14761 ttctgcactt tggacaatcc ctccaccagt cgcgataatt ttgtcttcat ttgacagctt
    14821 tcttaaaata tctgactcca cttttcgaaa gcttttttca ccgtaatagt gaaaatattc
    14881 ggcaatcgac aattggatat gttcctcaat ctttgtatct aaatctacat actctttttg
    14941 taaacattga gctaaccctt taccgattgt tgtttttcca gcgcccatga aaccaatcag
    15001 caaaatgcta gccatgagct tctcctcctg acacaagttc tgctaaatcg tcaaaaaatg
    15061 ccggataaga gattgaaaca gcttcagctc cttctagttc gcaaggtcca tcagctatca
    15121 aagcagctac ttgcagcatc ataccgattc gatgatcgcc tctgctggac acttttgcgc
    15181 catgcagagg tgtcggccca tggatgatca gaccgtctgc tgtcgctttt atctttgctc
    15241 ccattttctg taattcttct gccaccgcat caattctgtt ggtttctttt acttttaatt
    15301 cttctgcgtc tttgatgatc gtgattcctt ctgcttgagt agccactaaa gctagaatgg
    15361 gcaattcatc aatcagacgc ggaatgattt ctccaccaat cgttgctttc ttcaaatgac
    15421 tcgtcttgac acttaaatca gccgattggt tctgttcatt gtgattcgtc tgggtaatcg
    15481 ctgcgcccat ctcctccaac acatctaaga taccagttct tgttggattg accccgacat
    15541 tttttaacaa cagttggctt tccggaacaa gaagtcctgc tgctagaaag aaagcagctg
    15601 atgaaatatc acctggaaca gttatctgtt gaccagttaa tttttgcggt ccggtcacta
    15661 tgatcgtttt atcttccact gtgatccttc cgccaaattg acgaatcatt tcttccgtat
    15721 ggttacggga acgttctttt tcaacgatta cggatgtgcc ttctgcttgt aatgccgcaa
    15781 acagcaaagc agatttgact tgcgcactgg ctacaggcat attgtatcgg ataggtgata
    15841 aagagcgggt tcctgtcaca gtgattggta gatactggtc attttcttct ccttgcaagt
    15901 cagcgcccat ctcgcgtaaa ggttccatca ctcgccccat tggacgttta gacaacgaag
    15961 catccccgaa cagagtagtc gtaaaaggct gtccagccaa aatacccatc aataaacgta
    16021 tggtcgtacc ggagtttccc atgtcaattg attcggttgg ttggacaaat gagtggctac
    16081 cgcgaccatg aatcttgatc acctcttcct cttcttcgat ctcgacacct aattgttgga
    16141 aggcatgtaa agtactcaga caatcttctg cgcgcaaaaa attttgtata gtcgtcgttc
    16201 cttcagcaat tgctccaaac atgatgctgc gatgggaaat tgatttatct gctggtatcc
    16261 taacagtccc tcttaatcca tgtatttgtt gtaataattg catttatcca gcctcctatt
    16321 tgatccagca atgataactg gtattagaca taatgattct ttttgcttgt aacaaatcct
    16381 gttggttttt aaaactgatt tgcagtattc ctaaaatatc ttccctggtc tcttggatct
    16441 tcaaattaat caaagaaact cccgcttttc cgatcagtcc agttacttct gcaataacgc
    16501 caggttcatc tggcacatcg ataaataaat caaaaaaggc aggaatcgcg ccttgcttat
    16561 ggacaggcaa ctggtctcga ctttcttttg cccgattgaa aaaagaaaag atttccgatt
    16621 ggttctcagt catgatccat tctgctattt gtgtcatttc ttgttgccaa tctgccaatc
    16681 gttccaataa tatctgctta ttgctcaaca aaatatctgt ccacatgaca ggatctgagg
    16741 aagcgatcct agtgatatct cgaaagcctc ctgctgccag ttgtttggct cgcggatact
    16801 cttgattgaa ttgttctgcc tgattgacta agccagctgc cagaatatgt ggaaaatgac
    16861 ttagcattcc tgttatacgg tcatgttctc ttgctgtaag agttacatat tttgctttgg
    16921 ttccgcaaaa tagttcttgc agtttttcaa catctttttt cgtcttttcc aaaggtgtaa
    16981 agatgtaata ggcattttca aatagtttag catctgctgc ctgtatacct gatttatgag
    17041 aaccagccat tggatgtcca ccgacaaacc gaagattttt ctgctcagcg gatgtcacga
    17101 cttgctgctt cgtactgctg acatccgtaa tgatgacgtg atcagacaag ttgattcttt
    17161 caaggacatc aagataagct aaggttgtac ggacgggaac ggctaataag ataacgtctg
    17221 cttgttctgc acctgtttca aaatcacttg caacgtaatc aacgatctgc tgttctactg
    17281 cttgctcacg ggtttttgac tgactatccc aaccaactac tatcagttct ggattggtct
    17341 gacgaataca aagtgccata gaagcaccaa tcagtccaag ccccacgata aaaacatgtt
    17401 gtgccatctt atttgccccc tttttagtaa tgtttcaagt agtcacggta ctccttcact
    17461 gcagctttca actcaacaag cgaatcactt gggaattttg acaatagctc tgtcgctacc
    17521 acagtggcta caacattttc cgcaacgacg ctagctgcag gaactgctgt actatccgaa
    17581 cgttctacac ttgctttgta aggttctttt gtatcgatat caacactttg aagtggcttg
    17641 tacaatgtag gaatgggttt catgaccccg cgaatgacaa gtggttcgcc attggtcatt
    17701 ccgccttcga agccgcctag attattggta cgtcggaaat agcctctttc ttcttcccat
    17761 aggatctcat ccatcacttc tgagcctggt aatgaaccag aagtgaatcc gaccccaaat
    17821 tctacgcctt taaaggcatt gatactaacc acagcttgtg cgattttggc atctagcttc
    17881 gtgtcccatt gtacgtagct tcctaatcca gctggaacgc ctccaacgag cacttctaca
    17941 attcctccga tcgtatctcc atttttcttt gtctgatcga tcaaatcttt tattctttgc
    18001 tcaactgctg gatcaacaac tcgtacttct gacgcttcag aaatttcttt gatttcttga
    18061 atcgagatat ttccaggtat ttctgcttgg attccaccaa gacaaatcac gtgtcctgct
    18121 acgatgatgt caagttcttc cagaattttc ttggcaattg cgccgattgc tacacgcatg
    18181 gtcgtctctc ttgcagagga ccgttcaagc acattccgaa gatctcgatg atggtatttc
    18241 ataccgccta ccaaatccgc gtgtcctgga cgcggacgtg caactcgccg cagtgattgg
    18301 tctttttcag gcacttctgc tacagacatg acagttgtcc aatttttcca atctttattt
    18361 tctaccacta aagtgacagg tgaaccaatt gttttgccgt gacggatgcc agacgtgatc
    18421 cgaacacgat ctttttcgat cttcatcctt cctcctctgc cgtaaccagc ttgtctgcga
    18481 gcgagttcgt gattgatttc ttcttctgac agaggtaatc ctgctggtag tccttcaata
    18541 atggctgtta attctggtcc atgggattct cccgcagtca tataacgcat cttacagtcc
    18601 tcctaagtaa tctttgaatg tctctgtggg aacaggaacg atttttgctt gcccaatctt
    18661 ttctagcaag atgatattca attggcttcc tcttgctttc ttatcatggg ttatcgcgtg
    18721 gaataattca tctggatgac ttagatcgta cgtaatcggc agatggaatt tttgaagtat
    18781 cctttctaaa gcagctgtcg atccagttgg agacaagccg attttttctg catgagaagt
    18841 gatggctacc attccaatag ctactccttc tccatgactg actaccccat atcctgctgt
    18901 attttcaatt gcatggccaa ttgtatgacc aaaatttaaa ataagtcgat ttccttggtc
    18961 aaagacatct tcttccacta cttttcgttt gatttccaac gtagcagtaa tgattggaac
    19021 agcatgttcc agaagatctt tttcgtcttt cagctcgttc agctgtttcc aaagcgttgc
    19081 atctgctata gctgctgatt tgatgatttc agcgattcct tctcgtaaac gacgtaccgg
    19141 caaagttttt agaacctcag gatcaatcaa tacaccagct ggctggctaa atgttccgac
    19201 aaggtttttc gctttgtttg tattgactgc tgtcttcccg ccaatactag aatctacttg
    19261 tgcaagtagc gtagtcggaa tttgaacgaa atggatccca cgcatgaacg tcgaggccac
    19321 gaatgctgct agatcaccca ccattcctcc accaagtgca agtatcccat cgctgcgggt
    19381 aaaatcatta tctgctaaaa aatcgtataa ctcagctgct tgttctaaag atttactggt
    19441 ctcacccgcc ggtacgattg aaaggactgg ttcaaaatta gcttgtctca attggaacat
    19501 gattttttct gcatatagtg gagcaacgtt tgtatcagtg atcacagcta ctttttgagg
    19561 tctccaaatc gtttgaagcc atgatccagc ctgttctagt gcgccttttt caatatgaat
    19621 atcgtatttt tggttatcaa catgtacagt aagcattcgt ttcacttctt tcttttaaaa
    19681 agctggaatt aatcgagtgt ctgaatcttt tccattgctt ctttcatgat cgcaacttct
    19741 ttcatcatgc gtgagtaagt cttttcattc aacgattggg gaccgtctga ccaagcattt
    19801 gcaggatcag gatggatctc tacgatcatg ccatctgctc cgcttgccac acccgcacga
    19861 gccattggtg gtacaagttc ccaaattcct gtaccatgac ttggatcaac aatcacaggg
    19921 aaatgagtta attttttcat caatgggaca gcacttaaat caaacgtatt acgtgtcgct
    19981 gtttcgtaag tccgaatccc acgttcgata aaaatgacag gagatttgtc ttcgactgca
    20041 atgtattctg cagcattaag ccattcattg atcgtaccgg aaattccacg tttcaatcca
    20101 acaggtttac ctgttttacc gacagcagat agcagtttaa aattttgcat attacgtgcg
    20161 ccaatctgta ggatatcgct gtactcagcc accatatcga tatgcccttc atccatcact
    20221 tcagtaatca ctttcattcc aaattcatca gccgcttgac ggatgtattt caatccttct
    20281 tcttctaaac cttggaaagc atatggagaa gtacgtggtt tgaaggcacc acctcgcaga
    20341 atttttgcgc ccccggcttt ggccattcgc gcacattctc tgatttggtc taatccttcg
    20401 atagaacaag gaccagccat cgtcacgaaa ctgccgtcac caatttttac tccgtctaca
    20461 tccacgactg tattgccagg atggaattct cgagaagtca atttgtatgt gtttaaaata
    20521 cgcacagcat tttctactcc ttcatagcgt gtaaatgctc cttcttgtaa acttcgcgta
    20581 tcacctttta gtccaatcac tactcgttct gttccttgat cgattgagac tttgaatcct
    20641 tctttttcga tatgatcgac gacttttttt atttgttctt ccgttgcttc ttttttcata
    20701 attactatca ttgtttttcc cgctttcttt tgtttactta tctatcttgg ctgattttct
    20761 ctactttttc aaggtggttt tacattaatt cctactaaat gaccgtgtcc tgttgttctc
    20821 attttcgctc cttctttcct acttatgacc cagataatca acaaaaaaag atgtccctgt
    20881 aggaaattgc ctctacagtg aacatcttca acgagcctta aaaagcgctg caaaaatata
    20941 aaatagccac tatagatgaa tttctctacg tggctgtaag tcgtgtttaa aaatctttag
    21001 ccatcataga tacaaacctg gtttgtttgt acccacgaag tcatgaatac aaacctatct
    21061 aaaatagcta aagtaatacg tattcagttt tgtaagtttt ctagatttca taaaaaatcg
    21121 ctccaaactg atgtaagaat tgtacctagt atagacataa aaaaatcgac tgtcaatcat
    21181 ttttatgaaa ctttttttaa ttttctgata gttaactttg tttttcagaa aaataacaat
    21241 tgattttatt gaagaaaata gtcatgaaca aaagtatctg agaacttttt tactctcttg
    21301 ttcatgacat atagaataaa gcagctttgt cttgtaaact ccgtttttca aataataatc
    21361 taaagattgc tactgaataa taaagccgat ttcatggtcc tcttcgctgc tgattcggta
    21421 aggtgactca acatccgctc cccagctatt gatacccccg actcctctta cttttgcaaa
    21481 aatcgtaaga accgttcgtc tttctggcgg tagttcttct atatgtgttg cctgttccag
    21541 ttcttctgct ttgaatggta aagcggaaaa agcaaatggc gtatcctgct gttcaaatcg
    21601 caacgaaaaa tcttcttgat ctttatccgt attatccaac gtttgatttc ttgttagttc
    21661 tacccatttc gtttccatat gcatgccgca ttcttgggga acaaggtatg gggaaacggg
    21721 gagtcctgcc actttataaa taccttcgac accaccagcc atccgatccg gatatgtttc
    21781 ccctgatagg ccttcatact caaaaaattt tgctttcgtt ggcataatga accttaaacc
    21841 caatgcgggt aattccggta atcctttttg tccataataa tgcatattga caagcatttt
    21901 accctctcca gcgatttggt aggaaattgt cacttgggtt tttggtactg tgttgatttc
    21961 cagtgtaaaa atgatttcaa ccgtttcagc gtattcatta cttgaatatt gattatttac
    22021 tggagcaatc ggcaaatcga tctttttatt gtccaccagt acttctattt ctttgacttt
    22081 tggatacaat ccggctccta accacatacc ggatttaaat ccaaatccgc tacctctgtc
    22141 attatcggtc gttgctcgcc aaaaaactgg aagtgtttcc cggtatagcc attcttttcc
    22201 gtttttattg agcgattcca gtccgcctct tgtataagaa aaaatataat gcgtattccc
    22261 cgatttgaca cctaatgtac agtcgccgaa gacgattgca actttttccg tatccatatg
    22321 acttttattt actgtatttt tcataataat atcttacctc ctgcatggcg ggtttttctt
    22381 gtccatctgc aaaaacgatc ccattccctg agaattcata gtcacttggt ttatcgtcaa
    22441 agtcaccgcc atagcgcatc acttctctgc ctgtgattgg atcagtcact tggatcgcct
    22501 gatctttaaa atcccatata tagcctcctt ggtacatttc ataacgatca agcaacgtca
    22561 tgtaagaatc cattccacct aacgaattcc ccatgtcatg catatattca cataagatga
    22621 acggtttttc cgctttattt tcaaggtagt caatgatctc ttctggtggc gcatacatgc
    22681 agctttttac atctgaaatc gaatcttcga atgctggatt tggaaaattc ccttcataat
    22741 ggacaagtcg atcggggtct acttttttga agtaatcgtt catttgtcga atgttttctt
    22801 ccgcataaga ttcatttcca agagaccaaa aaagaatact tggatggttt tttaatgttt
    22861 cgaaattcgt ttttgctcga tcaagaacag ccgcctgcca ctgaggtact gcacctggta
    22921 cgttgtaaga aggctcaaat gcgcccattt tttgccatga gccatgggat tcgaggttcg
    22981 tttccgccat cacatggatt cctgcttggt cgcataaata ataccaagtg atctggttgg
    23041 ggtaatgaga tgtccgtacg ccgttgatct ttgcattttt tatgatagcc aaatcttttt
    23101 ccatatcttc tatagtgatg gcgcgtcctt ttttcggatg ccactcatgt cgatttaccc
    23161 cgttgataat catccgtttt ccattgaagt agatgatttt atctttgatt tcgattttac
    23221 gaaaaccgaa ggaatagtca atatactccg ttactctttt ttcttcgtct atcgtctcca
    23281 attcaagctt atataaattg ggctgaaaat gcccccacag tgccacattt tcaaatccat
    23341 cgatgcgaat cgtcatttgc tcttttaacg gatattgctg ttgatagatt tccttcccat
    23401 caggatctag tatcgctaca ttcagctgac tgtttttggc tacttgctca aaagacaaat
    23461 ctacttcaag tttgccattt tgacatttgt catctaagcg aggtttgatc caaagatctt
    23521 ctaaatggtt ttgtcctcga gcaactaaga agacatctct gaagatccca aaaaatcgga
    23581 aaaagtcctg atcttccaag aatgcagctg tacttctttt gtaaacctcc acagctagaa
    23641 tattttcttc ttccaaataa ggtgttaaat caaattcaga cggagtgaac gaatcttcgg
    23701 catacccgat aaaatgacca ttcaaccaaa taaacattgc ttgttctaca ccagcaaaat
    23761 gaatcgagat ttgttttccc tttagacctt cttctaatcg aaaagtcgtc aaatacgaac
    23821 caaccggatt gtctttagca gctgaaaaga tcccttccgc ctctttctca gggcttgtcg
    23881 tgaaaggggg acggcgaaat acatgtccct cccacggata catcgtattg atgtaatgga
    23941 tcttgtcata atttgctaat tcaatatgac taggaacagt gatttcatca aaagaacagg
    24001 catcaaaatc ttttttataa aatgtttttg gtcgtaccga aggatttggc gaataacaaa
    24061 aacgccatga tccatttaaa gaccgtttaa gggaggatgt ggtttctact tcttcccttg
    24121 ttgcataaca aacatgatcg ctatgtgcat ttgattggtt cactctaaaa acttgtggat
    24181 catctaacca ttttatattt gctttcatac ttttttcctt ctctctcata actttttgaa
    24241 atgaagcagt tctgtttgga aatcaccttg aaaatcgggg aagtaaagtc caactgtcat
    24301 caattcggcc cccgaaaata tccgcttcga ggtctgttcc tgataaagtg cctcatcatc
    24361 taaccccatc agatatactt catggaacgc tggttgagct gaagctagta tcctccctaa
    24421 aaacacaata gcttcatctt gtttaggaga cacaaacatc catgcggcaa tattttcttc
    24481 aaaaggactc gccaaacgat aataatggcc atactggatc accggacgaa tcgtttgata
    24541 ggatataatt tggtctttta cttgcttttt ttcttcagga gacagctttg tcaaatccag
    24601 ttcataacca aaaacagcgc tcatagctgt ttctcctctt gttttcaacg aagtagatct
    24661 gtcggtctga tgatttggta cagctgatac atgagctgtg atggacgaaa tcggataagc
    24721 aaggcttgtt ccatattgga ttttcatacg ctccactgca tccgtgttat cacttgtcca
    24781 tgactgcggc atataataaa taaacccggc atcaaaccgt ccacctccac cagaacagcc
    24841 ttcccataag atttccggat aatcagttgt caaatgttca agtagttcat aaagcccgag
    24901 catataacga tgcgctactt ctccttgtct ctgtgggtca agggtaatcg aatagacatc
    24961 tgaaagcgaa cggttcatat cccatttgat gtagtcaaga ggaatcgtat ctaaaatctt
    25021 tctcatttgt ttctcaatcg tctgccgaac atctaacctt gtaaaatcca gtacatgttg
    25081 tgatctggaa gcagaaggca ttctacctgg ctcctgcata agaaaatcag gatgtgttcg
    25141 atacagttca gaatcaaccg agatcatttc aggttcaaac cataaaccaa atttcaatcc
    25201 tttgctatga gcataatctg ctatttcacg taatccattt gtcaacttgc cttcatactc
    25261 gaaccagtct cctaatgaag aattgtccgc atctcttttt ccaaaccagc catcatctaa
    25321 tacgaacatt tcgatgccta gctcctttgc ttcatcaatg atcgccttga ttttttcact
    25381 attgaagtca aagtaagtgg cttcccagtt attgaccaga atcggccgtt cagcaaattg
    25441 atgacgacct cttgcgattc tttcacgtag caactcatgg tggatctgac tcattttgtt
    25501 caatccctga cttgagtaag aaaggatcat ttcaggtgtt tgaaaagact ggcctgcatt
    25561 caattcccac gaaaaacgat ggctgttgat cccaccgacg actctcaatt gattgatctg
    25621 atctttttca agttcgaaag aatggttgcc ggaataaaca aattgaagtc cgatcgcttc
    25681 tccagtattt tctgtcgtat gatgatgaac taaagcaata aagttattca tatggtgact
    25741 gctcgttcct cgacggcttt caaatacttt tgttccagat aaaacagatt gccgtgaaat
    25801 ctgtctttca ttgacatgtg ctcctggcaa agcaatgact tcatcaaatc gcctagtatg
    25861 ggcaaagtct agctggaaac tagccgcttt ttctagtttg attgtttctc cggtttcatt
    25921 tcttatcttt acagatcgtg tgatcactgc tctatcagta aatatggtat agaacaaatc
    25981 gaaataaatc gctgtttctc tatccttaag tgaaatcacc aacgtttgtg cctctgactt
    26041 gtccatcaca taagtttgag gcaagccttc gatctctggc ttcccatctg taatataatg
    26101 gctgtcatat ctcagatcca gtaagtctga tccattcttg cgctgaatga tcgatgcggg
    26161 cgttcgaaag tccatgctgc catgagaaga gtattcttgc ggtagtgtat ctttcgacag
    26221 agaccggtca ggatttaagg gaacatttcc tgaaaatcca cgatctcttc ggggataatt
    26281 tttattgtct ttgtactgct tgactggttt gccaaaataa atatgcgcca atacattgct
    26341 ttcttctatc tttatcagat aagaaagaaa tgtatttcgt aaatggaaga ctcggctctt
    26401 ttcatcaaat aagatgaaat ttttatctgt ttttctatgc gtaatatcca tcatttcact
    26461 cccaacaaaa cggaaaccgt ttactttttt ttataattcc tgtatactgt ttactaaaca
    26521 attttgtcaa taaaaatttt ttattttttt gaggagtgtg taaaaatggt tggaattaga
    26581 gatgtcgcac gaaaggcaaa tgtctctcct gctacagttt cccgtgtgtt gaataacgat
    26641 cagacgatcc atgtggctca gactactcgg caacggatag tagaggcagc tgctgcgttg
    26701 gattatgaca tcagtactcg taaatatatc aaaaagagaa tgccttcgat tggtgtgatc
    26761 tctacgatcg gcaaagcgag cgaagaacag gatatctatt acaaacattt gcgagtaggg
    26821 atggaagaag aagctcgacg tctgcattta gggatgaatc gaatctataa tctatctgat
    26881 aatcctaaac aatgggaaga tctagatcag ttaggtgcgg tgatcgttgt tgggactgtt
    26941 acgaaagaat cgattaccgc cttgttaaaa caaaacatgc aaatagtcgt cgtggataac
    27001 cctgacatcc agcaagaggt cgatatggtg tatgcagatc ttgaacggat gactaaaacc
    27061 gtactggaat tgttcttaga aaatggtcat aaaagaatag cctatatcgg gggatataaa
    27121 gtcgatatgg atgaattcgg tgggaagcag atttctataa atgaaaagag gatgcgtgcc
    27181 tatcagcatt ttatgatcga tcatcactta gaaaaatata tgtcttatca tctaggtggc
    27241 tggacagaaa aacaaggtga ggtacttgcg actaatctat tagaaaatga atcccctctt
    27301 cctactgcca ttcttgtcgg gagcgatcca atggcaattg gtgtctatcg ggcattgaaa
    27361 caaaaaaata tccaaattgg agaagagatt gttgtagcaa gctttgataa tatcgagaca
    27421 atcgcaaacc tctcccctgg cttgaccagt gttcaaatca atgcgaaatc aattgggcaa
    27481 gcagctgttc gaatggcatt ggagcgaatt gacggtatac gggacgaacc aatcattttg
    27541 acttatcctg cacgattgat tgttagggaa agttttatcc caagaacaaa aaggtattga
    27601 aagagaggtt gtgaaaagcc gcttcgtttc ttcatactca aaacgaaacg gctttttcac
    27661 aacctcttac ttatacttat tcacaaaatc caatgtctgc tggtaacaat tttgcgattc
    27721 ttttagcgtg taatcaaatt ggtattcatg ggtaatctct ttttttgtat ctgtataaaa
    27781 caaggattgc acgggtacct ttaattctgt caaccgattt tctaatgctt gaccttgctc
    27841 ctggaatgag tatgcatttc cgtcagttat atacgttggc ggaaattcct tagaaacatg
    27901 gtcagccaca ctagcttctt tcaattgctc actcgttttc caatcttttg ttccaatcaa
    27961 cgaccacgca acggttttga cgaaaaactt cataaatcga tcatccgaat gctgctgtgc
    28021 tgttagcttc aaatcaacag gtccgcaata agaaatggtt cctttcagac tatcttttgg
    28081 aagtagctgt ttgattttca tttgtttagc atacgcatca ttcgtttgta tagctgcata
    28141 ttgcaatgcg atttgtgaac ctgcactatc tccaccaaaa aataattgtt ccatgtctag
    28201 caatttgtcg tctttttctt cagctaataa atctttgatc agttcattca cttgaagaac
    28261 ttgattaggg tattctgaat caggcgctaa ttcataattc atcgcaacca ctgcgatgtt
    28321 ggcgtctgaa actaattttg tcgcaaattc tttgacaccc gatttatctc cgccgacgaa
    28381 tcctccacca tgtacccaaa atagaacagg aacaggcccc ttgctgtttt ttggatagta
    28441 gatgtcataa gtattctctt tatacttgga ctcatacgtc aaatctgttt ttttcgccac
    28501 gtttttttct gcttgatcga acgttttttt atcagtgatt tgaacagtat ttgaaaacat
    28561 atggttgatc aagaatgctc caggtctggg ggaaagctgg aaggcaagaa agattgcaaa
    28621 cgcgatccca agaatgattc cgaagaacca tccgataaat ttaagaattt tctttttcat
    28681 aaggctccct cagtagttag tattaagcat tctgattata ccatagtgtt atttcctatc
    28741 aagctctcct agatgattta atgattttcc ccaaacttct tctgcaatgc ttttgacaaa
    28801 agaaattttt tccattgttc ttcaaacaaa acattttctt cttctgttga agcaaatcca
    28861 cattgaggac ttaagcacaa acgatccaaa ggcagatatc gtgtcgcttc gtagatccgg
    28921 tcaatcactt gctggtgctc ttccatttcg ccatttttcg ttacagtcaa aatctaagac
    28981 aactaattta tcttcagaga ctttctctaa aagaacaaaa ctcccagcac gttcactatc
    29041 gtattctaaa taataggcac ggacattttc ttgagtaaaa taatgggtct gctacgtcgt
    29101 catcccccct gatgccgcca aaatcgaata ataatttccg cgtcaaacat gtatatttag
    29161 tgttgaatca tttgagaata tgaaaataaa aatctttttg atcaatcagt taatcaaatt
    29221 gaaataacga aaaatctgag acagaatgac gttcattctg acccagattt gattttgcac
    29281 aactcaacag tccttattgg caaatcgcca aaataatgta gactaactaa aaagactaag
    29341 aaacaataac aaaagttaac acttcttagt ctttctttag cacgtctatt tattaaagat
    29401 tttagggcct tttctcatgt ttttacgatc attcttaggt gtttccattt gtttggatcg
    29461 gcctccacct tgtttctttt tgatcaattc tttcattttt tctttttggt ccatttttat
    29521 tctcctttaa cgaatgaagc gactaattcg atatgatggg tttgcgggaa aagatctaca
    29581 ggctgaactt tcttcacggc ataacctgct tctgcaaata acttagcatc tcttgcaaaa
    29641 gttgccggat tacatgagat atagacgatt cgttctggtg acatttcgat tgctgattca
    29701 ataaaatgat gatctaatcc ttttcgtgga gggtcaacta cgatcacaga tggtttgatc
    29761 cctttttctt gccatttttt cataactttt tctgcttttc cgacttcata atgaacattt
    29821 tcgattttat tatttaaagc attgaactgt gcatcttcaa ttgcttctgg aataacttcc
    29881 atcccataca ccttatccac tctgtctgct aaagacaagc caatcgtacc tattcctgaa
    29941 taagcgtcga tcacaatgtc ttttttatct aactcggcaa actcgatggc tgtttggtac
    30001 agatactctg cttgttgagt atttacttga taaaatgact ttgctgagat tcgataggtt
    30061 tttcctaata gctgatcttc gatatagtct ctgccatata aaactttttc tttgtctcct
    30121 aaaatcacat ttgttttttc ttcattgata ttttggatga ccgaaaccac ttctggcaat
    30181 tcttcatgaa tagcttcagc aatttccttg gatttaaaga atttttcttt acgagtaact
    30241 aaaacgacca tcatttcatg ggaataatga ccacgacgga taatgatgtg acgaagctgt
    30301 ccttcatttt tttcttcatt atatcctcgt acttggaacc gttgcaagat atctctgact
    30361 actttgattg cttgatcgat gacaggatct tggatataaa aatcttcgat agggactaga
    30421 tcatggctat tctttttgta aaaaccggtg gctaactgtc catcgatacg gcgaacaggg
    30481 atctgtgctt tattacggta gccgaatggt tctgccattc cgaccgttgg aagtacagga
    30541 acttctggca tcttagcaat cttcgccatt acatgctcta cttgtttttg tttgaagaca
    30601 agctgttgtt catacttcaa atgggctaaa ggtgcgatac ctatgcgaag taatgcattg
    30661 ttttctacag gctgacgatc aggtgattgt gtaataaatg tttcgacttt tccatatcca
    30721 aacttattgc ctgcttttac tactagtatt tctacttctt ctccagggag tgcattttcg
    30781 ataaataatg gataaccatc gatttttgct acccccattc cttcataact caagtcgacg
    30841 atttcgactg tatatcgttc gtttttcttt actatttgct gttctgtcat attttgctcc
    30901 tttaagccta tttttcaatc ctactgaact ctactataaa tcagctaatt acacaactaa
    30961 aaccggcact gaccttacta cgttcagcac cggcttttga ttaattcttt tgattaggtg
    31021 tttcgtctgc tatttttccg tcaccgtcga tatcttcatc cgttaaacgt tcgacttctt
    31081 ttacgaattc tttgctgaca acctctaatt cttcttcatc tgtacctgtg attgcttcat
    31141 caggaatctg atcggtattg gcaaagaact caatgtgttg atgaagattt tggaaatgca
    31201 tcggtgcttc cccgccgtat tcaccatcta agttgatcat tactggtcga ttgatatcat
    31261 cttctgtttc aacgaacaaa ctgcttgttt tggtatagat tattcttgga tcatcgacat
    31321 gtcgaccgcc attcagcatg agtgctgcaa tatgcaagat ctcaaagata tttgctgtct
    31381 tcacaatgat taaagaaaac ttcccatcat ccaatttggc atctggcgcg atctgttcga
    31441 atcctccaac agagttcgtc agccctagga aaaacatcga agcatttccg atatattccc
    31501 cttcatcata cttcaagcgc attttgattg gctttactcg agggagcatc tctgctcctt
    31561 tcgcaagata agcaagataa ccaaaaatac tctttagttc tgatggaact tcataagtca
    31621 attctgtcaa atagccacct gcagcgatat tgatgaagta actgttatca gaaaggccga
    31681 tgtccatttt tacggtttgg tgttttttta tcacttctgc tgctgcttta atattatctc
    31741 taggaatttt taatgctcga gcatagtcgt tcgttgtacc agctgggatg atggccattt
    31801 taggacggtg ttcaagcgga gcgattccat taaccacttc gttgattgtg ccatcgccac
    31861 cagctgccac tactagatca aaacctagtt ctgcgactct acatgcttca ttcttggctg
    31921 aatctggttc tggtgtagtt gcataggcgc tggcttcaaa tcctgcttct tctaatgcct
    31981 gtaggatatc cgccaaattt tttttcaata attcttttcc cgaggtgggg ttataaatta
    32041 cgcgtgcttt tttcattttc agcctctatc gtttcgctaa ttcatcttta agtagcttat
    32101 tcacgacacc aggatttgct tgtcctttag ttgctttcat gatttgaccg actaggaagc
    32161 caaccgcacg atcttttcca tttttgaaat catcgacaga ttgttggttg ttatccaaaa
    32221 tttcattaat gattggcagt agtttgttcg gatcagataa ttgcacccaa ccattttttt
    32281 caactacttc ttgggcttct ccgccatttg tgatcaattc acggaagact tttttggcga
    32341 tttttgagct gatcgttcca tcggcgatca aacgaatcat tcctgctaag ttctcaggtg
    32401 tcaattttgt ctcagctaat tccagtttct cactgttcaa gtaagcagaa acttcaccca
    32461 tcaaccagtt agatgcttgc ttggcatctg caccattttc caaaacagct tcaaaaaaat
    32521 cagacatttc ttttgtaagt gtcaatacca ttgcgtcata ctctggaagt ccaaactcac
    32581 ggatatagcg ttcgcgacga gaagctggca tctcaggcaa actagctttt actttttgaa
    32641 tccattcgtc atcgatcacg aatttcggga tatcaggctc tgggaagtaa cgataatcac
    32701 tagacccttc tttgacacgc atcaaaactg ttttattcgt tgtttcgtca aaacggcgtg
    32761 tttcttgttg gatttctcct cctgaaagaa gaacttttgc ttgacgtttt tcttcaaaag
    32821 ctaaaccttt tttcacgaaa gataaagagt tcaagttttt caattctgct ttcgtaccga
    32881 actcttcttg tccataaggg cgaagagaaa tattggcatc acagcgcata gagccttctt
    32941 ccattttcac atcgctgact tctgtaaatt ggataattga tcgcaatgct tctagataag
    33001 cgtatgcttc ttctggtgaa cgcatatcag cttcagaaac gatttcgatc aaaggcgtgc
    33061 cttgtcggtt caaatcaacg taagaataac cgccaatacc atgcatgttt tttccagcat
    33121 cttcttctaa atggacacgt tcgatccgga tttttttctt tttaccttct acttcgattt
    33181 cgatccatcc gtcatgaccg attggttgat caaattggga aatttgatat gcttttggat
    33241 tatctgggta aaagtaattt ttccggtcaa aatgagtatc tttagagatt tcacagttta
    33301 atgccaaagc tgctttcatc ccgaattcca gcgcagcttt attcattact ggcagcacgc
    33361 ctggataacc ccagtcgatc acgtttgtat tgctgtttgg ttctgcacca aaatgagctg
    33421 gcgctggaga gaaaattttt gagttggttt ttaactcgac gtggacttct agtccaatga
    33481 ctgtttcaaa attcattcgt ctttccctcc taaaatagct ggttgttgtt tatggaattc
    33541 tgttgcttgt tcaaacgcat atgccgcttg atacatcgtt ttttcatcaa aataattacc
    33601 gatgatctgc aatccaactg gtaatccttc tgataaacct gccggtacag acatacctgg
    33661 aagtccagcc aagttgacag ggatcgttaa gatgtcactc atgtacattg tgattggatc
    33721 gttaatgttc tcaccgattc cgaatgcaac tgttggtgaa gatggcccga tgatcaagtc
    33781 ataatctgca aagacttttt caaaatctcg tttgatcaat gtacggactt gtcctgcttt
    33841 tttgaagtaa gcgtcataat aacccgcgct taaggagaat gtacctaaca tgatacggcg
    33901 ttttacttct tcaccgaatc cttctgaacg tgagttgaca tatacatcat ctaatgattt
    33961 cacattttca gagcgataac cgtagcggat accatcgaat cgttgcaaat tggaacttgc
    34021 ttctgatgag gcaatgatat aataaacagc cacaccgtat tttgaatgag gcaagctgac
    34081 ttcttccaca acagcaccta atgaacggaa tgtttctgct gctttcaata cagcttcttt
    34141 tacgccaggg gccacacctt cgcctaagta ttctttaggt aaagcaatct tcattccttt
    34201 gatgtcgcct gtcagtccct ctgcaaaatc aggaactgaa acgcctgaag aagtaccgtc
    34261 tttttcgtca taaccgctga tagctgtcaa tgctaatgca ttatctttca ccgtacgggt
    34321 cattggtccg atttggtcaa gagatgaagc aaaggcaatc aaaccaaaac gtgagacacg
    34381 gccataagta ggtttcatcc ctacgatgcc gttaaaagct gctggttggc ggatacttcc
    34441 cccagtatca ctacctaaag aaactggaat ttgtccagct gcaactgctg ctgcagaacc
    34501 accagaagag ccaccaggta cttttgttgg gtcccaagca ttttttgttt tcttgaaata
    34561 agaagtttct gtacttccac ccatagcaaa ttcatccatg tttagcttac caacagggat
    34621 catatctgct tgatagattt tatccatgac cgttgcatca taaattggtt caaaattgta
    34681 aaggatctta gatgcagcgg ttgtcaagat atcttttgtt acgatattat ccttgatacc
    34741 aattggaata ccagcaagga cgttgctttc atcaatccct ttttcatcta aagcttttgc
    34801 ctgttccaat gctttttctt cgttcaatgt aatgaatgcc tcgacctcag gctctgtttc
    34861 tttgattcgt tcaaatgttg tttttgtcaa atcttgtaca gtgatttctt tcgatacaag
    34921 catatgatgc agttcttcta gagaacgatt gtgtaattct gtcattatgc gccagcctcc
    34981 ccattatcga tgattgcggg tactttgata aagccgtctt ctgtttccgg cacatttttc
    35041 aataattcat cgcggttcca tccagcttgc gcccgatctt gtcgcatcac gtttactgtt
    35101 tcaaccacat tcgacgtaaa tggaacacct tccgtatcta cttcttccag catttcaacc
    35161 atatcgatga ttttacctaa ttggtcggta aaacttgcaa gttcttcatc tgcgaacgca
    35221 agttttgcta atttggcaac gtgtttcact tgttcttcac taatcgccat actacccctt
    35281 ctttctaaac gagttaaaaa ctttgctctc tattttaaca caagagtgcc gtgtctgaaa
    35341 ttgtttattg aaatttgtct gaaaaaactc atttttcatt cttctactga gcgactttta
    35401 gccaatgcat ctgccatttg atcttctgtc caaacttcga ttcctaattc ttgtgctttg
    35461 gtcagcttgc tgccagcatc ttctcctgct accacgatat ccgttttttt cgatacgcta
    35521 ccagtcactt tcccgccaag attttcgatc gtctcttttg cttcttcacg agtaaatcgt
    35581 gttagtttac cagtaaggac tactgttttt tctttaaaag gagactcgac ttcttgcaat
    35641 tgggcatttc gaagcccttt gtattcgaaa ttgacgcctg cttgttttaa ttcattcatc
    35701 aattcatgga cttcttcgtt actgaaataa gtgactacac tgtcggcaat gatatcaccg
    35761 atcgtatcaa gcgcaataat cgactcatag tcacttttac tcaatgtttc tagatcaccg
    35821 aaatgttctg ctaaaatttt tgctgctttc gctccgacat gtcggattcc taacccgaaa
    35881 atcaaccgct cgaccgaatt atctttgctg ttgtcgattg ctgtcaaaat attatttgct
    35941 gatttttctt ttattttgtc caacgtcaat aactcttctt ctgtcaattt gtaaagatca
    36001 gcaacatctg ctactagttt tttatcatac atctgttcta gtacgcgcgg ccccaaccca
    36061 tcgatattca tcgcatttct tgaaacaaag tggttcaaac cttctttcat ttgagcaggg
    36121 catttaggat tgatgcaccg taaggctact tcttcatcca aatggacaag ttcacttcca
    36181 caaactggac aatgtgtagg caattggtat tcttcgctgt ctttaggtcg tttatctaac
    36241 acgacttgcg aaacttctgg gatgatgtct cccgctttat aaatcagaac tgtatctttc
    36301 aagcggatat ccttcaactt aatataatca ccgttatgca ggctggcacg gcttactgtc
    36361 gttcctgcta ctcggactgg tgtcatgata gctgttggcg ttactacccc tgttcgaccg
    36421 actgtccatt cgatgttttc gattagcgtt tcaacttctt ctggcggaaa cttgtaagct
    36481 gctgcccatc tcggtgcttt gatagtaaaa ccaagttgat cttgtagcga gaattcattc
    36541 actttgatga cgattccatc gatttcatag ggcaaatcca cacgtttgtc atgatattct
    36601 tcaatgtatg accacacttc atcaatcgaa tgacatagac gtttttcccg attcgtgtgg
    36661 aatccaattt tttctagctc ttctagcgca tcatactggg tttttgcttg cataggaccg
    36721 aaatcagcta ctgtgtataa aaaagtgtcc agatttcttt tcgctgtgat tttagaatct
    36781 aactgacgca aagaaccagc tgcggcgttc cgaggattcg caaagatatc ttttccctct
    36841 gcttctctgt cttggttcaa ttggacaaat gaccgttttg gcatgaaaca ttcgcctctt
    36901 acttcgatgt tcattggttc ttttaatttg attgggaccg accggacagt ttttaggttt
    36961 tcagtgatgt tttctcctac agttccatcc ccgcgagtcg cacctctcac gaaatttcca
    37021 tcttcataac gcaaagaaac ggaaagtcca tctattttta gttcacagca atagtcaact
    37081 acacgtccca ctgctttttg tacacgttga tcaaaagcta tcagctcttc tttgctgaaa
    37141 acgtcgttca aactgtaaag cggaatatca tgagtgactt tttcaaaacc ttctaatact
    37201 tttccgccta ctcgttgagt gggagaatca gacgtgatca aatcaggata ttgttcctcg
    37261 attgctacta attctgcgta ttctttatca taaacatagt cttccactgt tggtttatct
    37321 tctacgtagt attcgcgaga ccattggttc agtcttgtac gaagttcttc tgctcgttta
    37381 gcagcttcgt caatcgtcat ttcatgttcc atcatttacc ctccactact aagctttttc
    37441 aattggcgca aaggcagcca aaagacgttt tactccttga cttgggaaag ccacatctag
    37501 ttctagatcc ttcgacgtcc cgcttactcg gacgactgtt cctagacccc attttttgtg
    37561 tttgacttta tctccaggtt tccagctgtt attttcccct ccagtagcac ttttagaggg
    37621 aacaaccgct gtctctggct gtttatagct atatgctgtt tttttagcga atacaggcgt
    37681 ttttttaggc gtaggacgca tgccttcgat ttccagtaac tcctgatcga tttcttctac
    37741 aaaacgagaa ggtcggttat attgcgtccg tccatataat gttctggaga aagcatttgt
    37801 taaatacaac gcttcttctg ctcgcgtgat ccccacatat gcgagacggc gttcttcctc
    37861 cagttcgctt tcttccatca atgctcgaga taaaggaaaa accccctctt ctagaccaat
    37921 caggaataca actggaaatt ctaatccttt ggctgcatgc aaagtcatca atgtcacttg
    37981 ggaagcatct tcttctaaat tgtcaatgtc ggaaacaagt gccagatcat ttaagaagac
    38041 cgttagtttt tcttctggtg catcagcgtc ttcttcattt tgctgttcga attgtttgtc
    38101 gaattcttgt gtgaccgtta aaaattcctc caagttttcg atgcgtgctt gtgcttctaa
    38161 tgtattttgg atttttaagt cttctagata tccgctacga tccaaaacct ctttcgtcaa
    38221 ttctgtcact gtcaagtatg ggatcatctg agtgacttct tggatcatct ctccaaatgc
    38281 acctaattgc tgtcctgctt ttccaccgat atttgccaga tccacatttt gcgctgcttc
    38341 aagcagtggc cattcatgaa gagaagcaaa actgcgtagt ttttcgatag atcctggtcc
    38401 gatgcctcgt ttgggcgaat taacgatacg ctcaaaactg attgaatctt gaggatttac
    38461 cagcacattc aaatacgcta aaatatcttt gatttcttta cgatcgtaga acttgtgccc
    38521 tccaaccatt ttataaggaa tatttgcttt caacagagtt tcttccatta cacgagattg
    38581 tgcgttcgtc cggtacaaga tggcaaagtc gccgtaattc cgatgattgc ttcgaatctc
    38641 ttcttgcatc cggtctacga taaaacgggt ttcgtctcgt tcattatctg cacggtagta
    38701 agtgatcttg tttccttctt tgttttctgt ccacaagttt tttggttttc gattgctatt
    38761 gttttcaatg acttggtttg ctgcgctaag gatgtttttc gtggagcgat agttttgttc
    38821 tagcaaaatt actgcagcat ctggataatc tttttcaaaa tccaagatgt tctgcatatc
    38881 tgcaccgcgc cagccgtaaa tactttgatc tgcatctccc acgacgcaca ggttcctaaa
    38941 tctacctgcc aataaattga ctagcgtata ctgtgcgtga tttgtatctt ggtactcatc
    39001 tacatgaata taatggaatt tgttttgata atacgtgagt gaatcaggat gttcttcaaa
    39061 taatcgaatc gtattcatga tcaggtcgtc aaagtccata cattgattat attgaagctc
    39121 tttttggtaa gcagcataac attttgccgc aatctcttcg taataactgc cttgcatttt
    39181 tgcaaaatcc tgaggggtct gcaggctgtt ttttgcttga gagattgttc ctaatatact
    39241 tcgaggatca tattttttcg gatcaatgtt cagttctttt agtatccgtt tcatcaaggt
    39301 cagctgttca gaagaatcga tgattgtaaa gttccggtta tacccgataa aatcaacatc
    39361 tcttcttaaa atacgcacac acatcgagtg aaaagtcgat acccagacat cttcgcctcc
    39421 agatgcgaga atcgcattga cacgttcttt catttctttt gcagccttat tggtaaaagt
    39481 aatcgctagg atgttccaag gattgacttc tttttcctca atcaaatagg caatgcggtg
    39541 tgtcagcact ctcgtctttc cacttcctgc acctgccatt agcagtaatg gtccgtcagt
    39601 gtgaagaaca gcttctttct gacgggggtt catcccgttt aataattcag ctttggatga
    39661 cattaacaac atcctttctt ttatcaatct tgttaattat agcattttct tggctttcat
    39721 taaactagct agttcaaaat catttgtttc tttttatgga tttttccttg tcaattcgtt
    39781 gatttcgcgc taaattaaaa gaaagcgagg cgtttgaatg gaaaaaaaag gaaaaatcaa
    39841 gaaattagat aagactcaga tatattttat tctaaaagga atatttgttg gactgctgac
    39901 aggaatcgtc gttagcctgt ttcgattatc tatcgaaaag ctaagcgatt tagttcgaag
    39961 tatctatgaa atgagtcggg aacagccgat ttacttaatt ggcctagcta tttgctgcat
    40021 ccttgcagca tttttcgtgg gttatctggt caaaaatgaa ccggatatca agggaagcgg
    40081 gattccacaa gtagagggac agcttcgcgg ggaattatca atgaactggt tctctgtttt
    40141 atggaaaaag ttcattggcg gtgtactttc agttggtgcg ggcctttttc taggtagaga
    40201 aggaccttct atacagcttg gcgcatccat tggacaagga gctggacaat tgtttcggag
    40261 tccatcttca gaagaaaaga ttttaatttc tagcggtgca agtagcggac ttgcagcagc
    40321 attcaatgct cctatcgcag gattactctt tgttttagaa gaaatccatc actccttttc
    40381 tccacttgtt tggctgacat catttgcttc agcaatcact gctaacttcg tctctcttta
    40441 ctttttcggc ttgagacctg ttttatatct aggtgacctt ccttctttgc cgttgaaata
    40501 ttatggttcc ttagtgatct taggagtact tctaggcatc ttaggtttta tttatcagaa
    40561 tgtcttgctg gctttgccaa aattttacaa ccgattgcct ttaccagctt attttcacgg
    40621 gttcgttcct ttcttgctga ttttgccaat tggttattta tggcctagta ctctaggcgg
    40681 aggcaatcaa atcgttttag cttttggaca tgcttccttg ccaatcatta tgattatcgg
    40741 tttgtttgtt cttcgattcg ttttttccat gatttcttac ggtgggggtc ttccaggggg
    40801 gattttcctt ccgatattga cattgggggc tttaatcggt agcttttatg gaaacattat
    40861 ggttgatttc gggatggatc caatctacat caaggatttt gtcgttattg ccatggctgg
    40921 ttatttcaca gcgattggaa aagcaccgct gacagcaatt attttagtga cagaaatggt
    40981 tggctctctt aatcatctga tgcctttagg attggctgct cttgttgctt atatcgtcaa
    41041 tgaccttctc ggcggtaatc caatttacga atcattattg gaacgtctac ttggaaatac
    41101 tcagaccgcc tcactaaaag gtgcaaggac aacttttgat tttccagtaa cggcagagag
    41161 ttctttagac ggattaatgg tacgagactt caattggcct aaaaatatgc tgctaatctc
    41221 tatacgcaga ggaaatcagg aaatcttgac tcacggtgat acgatcatga atgtaggaga
    41281 tattttggtt atcttgactg atgaagcaaa cttgccaaga atcaagcagg aaatcgaggc
    41341 taaatcttct atcacaaatt tgaagcaatc attttaacga acatggaaaa actattttac
    41401 aacaactatt catctacact acttgattct atatgattct ataaaaaaag aagaggctgg
    41461 gaaaacccag cctcttcttt tttatttcta tgcttctgct actgcaggag cttttctcaa
    41521 taaaccataa atcacaccag atacgactgc tccgatagca ataaacacca aatacatgac
    41581 cgggtgactc agtaagaata caacgaaaat cccgccgtga ggagccagta atttgattcc
    41641 gaaagctccg actaatccgc cagttaaagc agaaccaata ataaaacttg gaatggcacg
    41701 aatcgggtca gctgctgcaa atggaatcga tccttccgtg acaaacgata gtcccatcac
    41761 gatgttcgtc aaaccagcat cttgatctgt tttgctgaat ttattcttaa acaaacgtgt
    41821 agcaacgaaa attgccaatg gaggtaccat tcctccagcc ataacagaag ccatcacgac
    41881 acttccgcca gttgcaacag tcgtagctaa agttgctgta ccaaacacat aagcggcttt
    41941 gttgattgga ccgcctaaat ctgccgccat cattccagct aataatgcac caagtaaagc
    42001 agcattcgaa ccactcatac ttgtcaagaa accgttcaat gcatcattga ttgctttcat
    42061 agggacattg accagcaaca tcaagaaacc agtaatcagt aagccaaaca ctggataaaa
    42121 gaggatcgtt ttaattccat ccaatgattt tggtaagttc ttgaataatt tacgtaaaaa
    42181 tacgatgaca taaccagcta agaatccacc aatcagtgca cctaagaatc ctgcaccgcc
    42241 tgtattagct aatgcaccag cagcaaatcc aacgatcaaa ccagggcggt caccgatact
    42301 tgaagcaata aatcctgcta ataccggcag catgaaacca aatgcagctt gtccgatttg
    42361 gttgaaccag ctagcagctt gattgtaatt ccctagattt gataattgat catgtggcac
    42421 gccaataaat tggtcgatca tgaaagaaag cgcaatagcg attcctccac caataacgaa
    42481 aggtaacatg tgtgatacgc cgttcatcaa atctttgtaa attctttgtc cgatagtacc
    42541 atccgcattt ccttcttctg aactgctttc ttttccatca ccatggaata ctggtgcagt
    42601 accactaagg gctagattga tcaactcttc tggtttacgg ataccatcac tgacaggacg
    42661 attgatcagt tcttttccgt caaagcgatt catctctact tttttgtctg ctgcaacgat
    42721 tacaccttct gcacgggcga tgtcttcagc agtcaatcgg tttttgattc cttctgaacc
    42781 gttcgtttcg actttgatct ccacacccat ttctttcgct tttttcttca aagcatcttc
    42841 tgccatgtaa gtgtgagcga tccctgttgg acaagctgta acggcaacaa cgaattttct
    42901 ttctggatga gcagaagctt ctgcagcttg tgtttcttgt tctgcttttt cttctgcttc
    42961 tttttgctct tcagctgttt ggaacaagtc ttggacttct tctggtgtat ttgcttcttt
    43021 taattttcct acgaaatctg catcaatcag caatcgagat agtgctgcta atgcttgcaa
    43081 atgtgtgtcg tttgcgcctt ctggagctgc gatcatgaaa aacagataag ttggctgtcc
    43141 gtctaaggct tcataatcta ctcctttttg gctttttgcg aataaaactg ttgcttcttt
    43201 aactgcactg tttttcgcgt gaggcatggc gattccatca ccaagacctg tagaagtttg
    43261 tgcttcgcgg gctagaattc cttctttata tgtttcgata tcggaaatcc ggccggcatc
    43321 aaacattttt tggaccattt catcgattgc gcctttttta tcagtcgctt gcaaatccat
    43381 gatcatcgct tctttgacca ttaaatcctt gatattcatg attatcctcc ttgaaactaa
    43441 tcaatttttg tgatttcaac ttctggcagc aactcatcaa tcaattcgcg agctgccaag
    43501 tcatcagaaa aagctgttgc gcttccgcaa gcaactcccc atttaaaggc ttcgattgga
    43561 tctttcgtac ggctgtaagc cccaataaag ccagcaatca ttgaatcccc tgcacctact
    43621 gaatttttca atgggcgaac taatacattc gttttataag ttccttgttc cgtgaagagc
    43681 aaagcaccgt ctccagccat tgagatcagc acattctgtg ccccttcagc tagtaatttt
    43741 ttcccatacg gaatgatttc ttctacagat tggaaggttg tgttgtaaag ctcagctaat
    43801 tcgtgattgt ttggtttgat caacaacggt ttcttaacaa gtgcattttt taggtcatca
    43861 cctgttgtat caatcacgaa atctgcacct ttttctcgaa tgatatcaat cagttcttga
    43921 taaaatcctt ttcgcaggct agctggcgcg cttcctgaca atacgacgat atctccttgt
    43981 cctactgttc ctaatgcttg cttcaactca tcgatttctt catctgaaat agatgggcct
    44041 aagccattga tctcagtttc agtgtctgat tttagtttga tattgattct tgtatcttct
    44101 tttacggttg tgaacaaaga ctggatttgt tctttttcaa gccacttcga aataaattcg
    44161 ccagtgaacc ctcctaaaaa ccctagtgct gtagattgag aatcgatccg tttcaaaata
    44221 cgggaaacat tgatcccttt tcctccaggt aacttgaagt cttccttcat tcggttcaaa
    44281 tcgcctagtt ttaacccatc tacgcgtacg atataatcaa ttgatggatt aagtgttact
    44341 gtgtaaatca ttgtattgcc tccttaattg ttgtcttttc acggaattca ttaacgaatt
    44401 ctaatggaca acgctcggtt atgatcgtgg ctgcttccaa aggtgcaaca gaggtgaagg
    44461 acaactggtt gaatttggta tgatcaatca atacaaatgt ctcttcggaa tttttcatgg
    44521 ctaaacgttt aacagctgcc tcttctggat ccggagtcgt gaatccttgt tcaagatggg
    44581 caccgttcat tcccataaat gctttattaa aatgaaaatt ttttagttgt tctatcgcat
    44641 tagatcctaa aacagcatta gttgccagct ttatcgttcc acctaaaata atggtagcaa
    44701 gctccaaatc aactaaattt gccgcatgtt gaacggaatt tgtcactact gtgatctgtt
    44761 ttccttgcag aaaaggaatc atttctaacg tagtagagcc agcgtctaaa taaatgacat
    44821 cttcgtcatt aatggtttca gctgccgatt tggcgatttg ctgtttttcg tgaacgtttt
    44881 tgagtgattt ttcactcata ttctgctcaa aattcaataa ttgctctttc ttagctccgc
    44941 catgaactct ttctagaaac ccactattct ccagctcttg taaatcacgt cttatcgttg
    45001 attcagaagc attcaaaagg tttacaagat cttgtgattt cacgattttc tgctgttcaa
    45061 gcaagtctag gattttttgt ctgcgttcct ctgtaagcat tttcacgatc acctcaatac
    45121 aaataatagc atattttttt tctactagca atataaatca gtcaaaaacg ttcataaaaa
    45181 ttataaatca gtcaaaaaaa aataaaaaac cagtcagaaa gactggtttt ttattttttt
    45241 gtctatcatc aagccgtttt atcgtttttt attttcgctt cttgtttttt tcatataaac
    45301 agaagcaggt tttctttcct tatgaagaag ataataataa agtcccatcc cgccaaacat
    45361 gaccaaaaaa ccgatgactg atcgaatagg aaaattagga acaaaaaaga gacagacgga
    45421 taataatgat gccggaacca ttattcgata atagagttgt ttatccattt tcttcacctc
    45481 atattttttg taaagtaatc aattgttcta attctcggta atctaaaaat tgcacacgca
    45541 gatgctgctc acctttcttg acatcgaatt ggatacttcg ccgctttttc tttattcgtt
    45601 tgaaggtaac atcagagagt ctatgtttga tttcctttaa cggtaattct gacagggatt
    45661 ctgaaggaac atagattctt aaggataaaa gatactcttg atatttttca tctgttacct
    45721 ctacttctag atattcccta atataataac aataagcttc gattccatta tccaaagcaa
    45781 cagtcggatc gataacttta tagcacatca tgcgtgatcg attctcgtca acccaaggaa
    45841 aatacgcctg agcatttagt gttccgcgaa cacgcctttc tttataatct ccttgttgga
    45901 tatcataact ttttgttaca aaaaatgaag cattcttttt aggaacaggt tgcatttgtg
    45961 cattctgata ctggacagat tggatcgtat ggtcagtgta aatgtattca acgaatggcg
    46021 gtttcaataa accgacacca ttgtcccaag caagatgatc aaacccaagc tggtccctaa
    46081 aataggattg aaacatttga ttgtttggct tttgccagtt aaaggggtct cgataaagct
    46141 ggatacatat gacttcgttt tctaattcct tcactttttc tacttgttgg atgtcgagaa
    46201 aaatcggttt agggatgatt tgatgataat agcgactata atacatttcc caacacgaag
    46261 tgaaacataa accttggtaa aaaagatcgt atcctggaaa ttggttgcaa tcgactacta
    46321 cttccccgtt ttgttgtttc attttaggta aatgttcgat ttcatccttt ttctcaaaca
    46381 atagccgttg agagatctgt tttgtattgt gatagataaa ctcgctataa gctcgcatat
    46441 aggcgaacac cccatgagca atcatttttt tctgaatgaa gtctcgaatc aaaaattgct
    46501 tttctttgaa aacagagata gacaatacat tttttatcaa taattgatta tcttccaagc
    46561 gaaaactaca ttcgttctcg tcatttccta caactaacca actttcgtct cttaccgatg
    46621 ctagcattat tttttcttga agctttcgaa gatcatacgg atccgcttgt tgtgtgaaag
    46681 agcggataaa tgttggctgg atcaacagac gtgtcaaaat agcccaatcg cttatttcct
    46741 gttttttttc ttcttcattt aagtctgtga gctggatagt catcaataat ttatcttcgc
    46801 caatcatttt ctcacctctt tcaaatcttt ttataattta attataacca atgataaaga
    46861 aaaactaaaa ctttatgtct tagaaaccaa tttggacgat tacatgatag actaagtaaa
    46921 aagaaacaaa gaggtgttta caatggggtt gaaatttgaa cgtacagata atttagataa
    46981 acttttcgaa tcatttgctg tcgatccgga aaaaacagtt gcaccaaaag aagagatcga
    47041 tcgatttcta aaaccacagc cacaaaatga aaagaaaaaa gacaagaaac aaaagtaagt
    47101 taagctcaaa aaaagactta ccagccaaac ttttatgaaa gagtttgact ggtaagtctt
    47161 ttttattctt tttatgattc aattgcttgt tcgattgctg cccacgcttc ttcttttccc
    47221 tgttttgtaa cggaagaaaa gagaataaac gtgtcattgc gatcaaaatc caacgctttt
    47281 ttgatttcgc tctcatgctt gttccattta ccacgaggga tcttatctgc cttagtggca
    47341 acaacaatta cagggatatc ataatacttc aaaaattgat acatctggat atcttcttga
    47401 gaaggcttat gacgcaaatc tactaaagaa acgaccgcac gtagctggtc tctagtcgtt
    47461 aaatatgtct cgatcatctt tccccatttt gctcgctcag ttttggatac ttttgcatac
    47521 ccatatcccg gaacatcgac aaaatgtaat gcgttctcga tcaaataaaa attcaatgtc
    47581 tgggtctttc ctggcttacc ggaagtacgg gctaaattct ttcgatcaat caatgtgttg
    47641 ataaaagaag atttccctac atttgatcgt cctgccagag ctatttctgg caattgtgta
    47701 tctggatatt gttttggtgc cactgcactt atgataattt cagcttgatg gactttcatg
    47761 gtctctctcc ttaacaaggt gatgaataaa gcaagcggct gtcgcaaaag ccaaaatgac
    47821 ttttacggca gcctcttttc atcaacccgc ttttttctct gaatctttat agattactgt
    47881 tggctcacca gacaattcag ctgcttcttt tgtgataatc actttttcga tgttttcatt
    47941 tgaagggata tcaaacatca catccatcat gatttcttca atgatggaac gaagtcctcg
    48001 agcaccggta ttgcgttcga ttgctttttt cgcaatggct ctcaacgctt caggttcgaa
    48061 ttcaagttcg gtattatcta gagaaagaag tttttgatat tgtttgacta gcgcattttt
    48121 cggctcagtc aagatccgta ccagatcatc tgttgtcaat ttctctaaag ctgccataac
    48181 aggtaatcgt ccgataaact cagggatcaa tccgaatttt agtaaatctt caggaatgat
    48241 atgctgcatg acactttcgt cttcagaaag tttttggttt tgtgtaccaa atccaatcgt
    48301 cttttcgccc atacgattct tcacaatcgt ttcgatgcca tcaaatgcac cacctacgat
    48361 gaacaatatg ttcgtcgtat caatctgaat aaattcttga tgtggatgtt tacgtccgcc
    48421 ctgaggtggc acacttgcaa cagttccttc tagaattttc agcaaggctt gttggacacc
    48481 ttcgccggaa acgtcacgag taatagatac attttcgctt ttacgcgcaa ttttatcgat
    48541 ctcatcaata tagatgatcc ctttttctgc aagttccaca ttgtaatcag cagattgaag
    48601 caatttaagc aaaatgtttt ctacatcttc ccccacataa ccagcttctg tcaaacttgt
    48661 cgcatctgcg attgcaaaag gtacattcaa tgtttttgct aatgtttgtg ctaaaaatgt
    48721 tttaccagac cctgtaggtc cgatcaagca gatattgctt ttttgcagtt caacatcatc
    48781 tgtgttttct tgtgcttgct gattgacacg tttatagtga ttgtatacgg caacagctaa
    48841 cgagcgtttt gcgcgatctt gtccaatcac gtagttatta aatacttcaa gaatttctag
    48901 tggtttaggg acttcagtga attcgcgtac agcttcttca taaaattctt catcaatgat
    48961 ttctttacat aggtcgatac attcattaca gatgtagacc cctggaccag ctacgatctt
    49021 tttcacttct tcttgggttt tcccgcaaaa agaacagcgt acggtctcat tgctacttgg
    49081 attgtcatac atggtcttca ccccttaact tgcaaaaagt atgcaagaaa ctatcaaaga
    49141 ctttacagtc ttttactatc ataacatagt ttagtaaaaa gaaaaagtgt ttaacaaagg
    49201 atcaactgac ctataccttt gcatttttta ctcttaacaa aaaataagga actgtgacaa
    49261 aatcgtgtca cagtccctta tttcgaataa acaattttct agaagtagct tcttcagaaa
    49321 tgatttatta cttgatttct attctttcgc ttctgtcgct gtacctgtga tcaattcgat
    49381 tgcttttttc attgtcacat catgttccag catatcttct gtcaacacgc gtttgatttg
    49441 ttcgattggc atgttgtatg tttctgataa ttcttggatt tctgcattga tttcttcttc
    49501 tgttgcttga agattttcag cagctgcaat tgcttcgatc acaagatttg ttcttgtgcg
    49561 catttctgct tcaccttcga attgtttatg caagtcttct tctgtagaac cagttaattg
    49621 atagtacatt tcaggagaga taccttgacg ttgcatgttg ttcaagaatt cgtccattga
    49681 acgatgtact tcatcatgaa ccatcacgtg tggcaattca acgatttctg cgttttcaac
    49741 agccatacgg atcgctgctt catcttttgc atcgtccgct gctttttctt ttgtttctac
    49801 taattctttg cgatattttt ctttcaattc gtctaatgtt gatacttcgt catcaacatc
    49861 ttttgcgaat tcgtcatcta attcaggcac ttcttttgct tttacttcat ggattgtcac
    49921 tttgaagact gcttcttttc cagcaagatc ttctgcttga taatcttctg ggaaagttac
    49981 atttacatct agactttcac ctgatttatg accaactaat tgatcttcaa aacctggaat
    50041 gaatgagcct gaacctaatt caagtgagta attttcgcct tttccgccat caaacgcttc
    50101 gccatctaca aagccttcga agtcgattac tactgtatcg ccattttcag ctgcagcatc
    50161 ttctttgata actaattcag cttgtgcttc ttgttcacgt ttgatacgtg catccacgtc
    50221 agcatctgtt acttcacggt cttgtttttc aacaactaga tctttatatt gtcctaattt
    50281 tacttctggt ttcactgtta cttcagcagt gattacccag tcttgacctt tttccatgct
    50341 ttcaacatcg atttttggtt gagaaacagg atcgatgcca gcttctttga cagcagcttc
    50401 ataagcttct ggtaatacag cattcaatgc atcttcatat aaagcttctt caccgtacat
    50461 acggttgaat acttgacgag aaactttccc tttacggaat ccaggtacgt tcaagctttt
    50521 tttcacttta ttaaacgcag ttgttaatcc tttttggatt tcgtcttgcg caatcgtaaa
    50581 ttttaaaaca ccatcattcg tgcctttttt ttcaaatgtt gcagtcattt aattccctcc
    50641 gagtttagaa attcatttta tacatcaaaa acgtttcatg cataccttta aatagtacac
    50701 taaccctttc aaattgtaaa catattgaat ggatagaatc cttgtttttc ataacatttt
    50761 tatctttttc actgataaaa tctaatggaa gaagatcatt tgctgcaaaa ttttttctct
    50821 tttttttaaa aaatcttgtt tttcatcttc tgtaccttca tacatcgttt ttcctgtaca
    50881 cgcctctaca gtcagttgaa accagcgctt cgggtcttcg atgtatgtat catagacagg
    50941 ataaacaatt gcagattgca acgtgaattc ttcttttaac acggaactaa gcagcgcatc
    51001 ttcattcccg taacgatctg aaaatacaca taattgctgg taacttgcct gctgctgcgg
    51061 ctttggcaga tcttttggta tgactttgac taacttttct tcaatcgtca aatagaaaac
    51121 aggatggcat tcgttcaatt gcctaagtgt ctccagtaat tttgcccttg ctaaaatatg
    51181 gacctctggc gatacaataa gtttactcgc tgtttggata aactcatgat aaggaagctg
    51241 ttcgatctca tcaatagacg caagctgacg tgcaggttcc aacgaaggaa gttcctccga
    51301 tttattgtgg agtgccttga tcttctgcct ctccatttgt ccggataatt cttctgcaaa
    51361 ttggattttt tcaatgaccc gctgctcgaa agcttcataa aaatctgtac ttgctatcaa
    51421 ttttctagca tataaaaaat cttgtgtact gattaacaag tcaaaataaa attcagcaaa
    51481 ctcctcattt tgaagatact gtttttcatg caagagtgct tgcttcaatg cttctttttt
    51541 ctcgtccaac tcgtaaagac aaaaaacaag caggctatta gcctcaaagc tttctttcaa
    51601 ctcgtaagct cgttgaaata attcttttgc ctgctgatat tgctggtttg ctagtgcttg
    51661 ttgtgccact cgcattaaat tctcatattt ttctggaaaa tcaatcggct tcacgcagtg
    51721 tcctcctctt gctcatattc agctcatgtt caatagtagc aaaacaaaaa aagcatgaca
    51781 attaaaaata actgaagcac cctctaaaag ctagatttta gatctgactt ttagaaggtg
    51841 ctttcaactg ttactgcttt ttattgctca tcagctgttg acttcctggt tgaacgcttt
    51901 gatcagattg atcgtaacaa cacttgtatt agtgaaaaaa tcgcccagat gttgcttttt
    51961 ctgattaaag actgctggaa gataccctaa catcagcaaa ggaaatttta gaatgaaccg
    52021 gcaagcacct tctctaacta aaacggttga ccagctaagt tcttcttcat caaagctgat
    52081 gactcgaata ccaaagatca ttttcccaat cgtttggcca tgattgaatt tcgtcaataa
    52141 aataaaatac gctagataaa taagtaaggc tgtgaatccg tagagactca gataagaatc
    52201 tgaaaaatta agccaaccta gatttttggc aataccaagt gttaaacgcg taatagctcc
    52261 gatacacaaa agatcgacca agaaggcaaa cattcggata aaaaaacctg catagaaata
    52321 cttaggaaaa tcatttgctt ttggcggttc atttcttgtg tccgtatact ttttccattt
    52381 acgttgtttc tttttgattt cttcgggcgt caaaggctct ctttcaaaag agctcagtac
    52441 cttttgggaa aagttggagt caggtcctac agcaggctgc tgttgcttat ctttttcttg
    52501 ttccttattt tcatttgaaa gctctgacat gcttactcac ctccgtaata atacattgct
    52561 tttggtgatt ccgctgtccc tagattctca agaacggata agatctgact agtctcagaa
    52621 ggcttcaatc cctggaattc tgctagttta cttcctaacc atgtactagc aaacccagat
    52681 ggcgtggaag aatattctac tagttccgca tcttcaagct gttgttcctt tcgtatagct
    52741 tttaaagcat cttcaggaaa tcccaattca tcgactaaac ctacttcttt tgcttggaca
    52801 ccatcataaa tccttccatc agcaattttt ttgacttctt cttcagattt attccgtccc
    52861 tcgctgacga ttgctacaaa acgactatat gcgctgtcga tatatgcttg aagcaccgct
    52921 tcttcttctt ttgtctctgg tcgcgttcct gaacccatat ctttcaacgc tccgctttta
    52981 actgttgtat cttccacacc cagtttttct agaagtcctg aataattcaa tcctgacatg
    53041 atcacaccaa ttgagccggt cactgtttct tctgttgcaa agattttatc tgcttgagca
    53101 gaaatatagt agccaccgct agctgccatg tttttcatgc tgacatacat tgggatatca
    53161 tgttctttac gaatcgtatc caatttctta gcgatttccg cactttcgta gacaccgcca
    53221 ccaggagaat ttacttctaa aaagatacct ttgactgttg gatcttcttc aatcgccttc
    53281 agttgttcca taaaattttt atgattgtaa ccttcagaag caaataaacc tgaagaccct
    53341 cccgcagcaa tcgttccatc tactgataat tttacgattt tttgcgtact gtctccctct
    53401 tccaacacat ttggagctag ttcttcatcc ccatataacc aactgttcaa tccctcaatt
    53461 tgatcgtttt cctgaggctt agtcaaacta gctgaaacta acgaaaccac aaataatcct
    53521 gcagcaatca gaactgctgc ccaacgtctt ttattcattc tttctccttt ctaatacaag
    53581 ttttttatga cgatcacagt ttctgactgc tcagaagtgt cgaaccgctc tgctttttct
    53641 tcccgcgcat acccggaaaa cgactctttt gttaattcat cttctaccgt aaactcacga
    53701 gcttgagcaa cttccaaggc atcaggtgcg atttcttgga tgctatccca gccagtatct
    53761 aaaaatattt tcgtatcatc tgatacacca gctgatgctt cgaatttgga caattgtttt
    53821 tttaacattc ccagtgtcat gatctcaatt tttgaatcca tttttactcc tcctctatcc
    53881 gattactttc tattgtaccg aactcctgaa aaagatgata gataaaaacc cattattttt
    53941 tgacataaaa aggacatttg acaaagaatt tgatatatac caatttattt tttattcttt
    54001 ttttgttgac aagaacattc gcaaaaggtt atgattttaa atgtttccag ataaaaaaca
    54061 aaaaggagga tagttatacc ttttaggata gcgccaggtc tgtgtaaaca gatgacgagg
    54121 agagggttta tcgaaagatt cggcggatgg ccctagggac tgcactctac agaaaatcca
    54181 caaaaaatgt ctgcaaagat aaaacaaaag gattttcctg cagctggaag cattcatacg
    54241 aattcattat agaaagtagg atgtttttta tgtgcggaat tgttggaatg attggattaa
    54301 aaaatgtaac accaggattg attgacggtc tagaaaaatt agaataccgt ggttatgatt
    54361 cagctggtat ctttgtctca gatggcacaa ctgattatct ggtcaaagca caaggcagaa
    54421 ttcaaaattt gaaaaacaaa ataaatattg atactacagg aaatataggt attggtcata
    54481 cacgatgggc aactcacggg caaccttctg aagaaaacgc ccatccacat acttcccaaa
    54541 gtggacgctt cgtgctcgtt cacaacggcg taatcgaaaa ctttgaagaa cttaaaattg
    54601 cttatttagc aaatgaccac tttattggtg agaccgatac tgagattatc gctcatctaa
    54661 ttgaaacttt cgcgaaggat actacaacgc aagaggcgtt tttaaaagct ttgcgggtca
    54721 tcaaaggttc ttacgctttc gctttgattg atcggacagc acctgatgtt atttacgtcg
    54781 ctaaaaataa aagtccacta ttaatcggac tcggcgatgg attcaatgtg attgccagcg
    54841 atgcaatggc tatgcttgct catacgaagg aattcgttga gattgaagat gaagagatgg
    54901 ttaccgtcac atcagaaaaa gttattattc aaaattttgc aggtgacatc gtagaacgtt
    54961 cttcattcga agcacaagta gatgcttcag atatcgagaa aggtacctat cctttttata
    55021 tgctgaaaga aatcgacgaa cagcctatta tcatgcgtcg tatcgctcaa aaatacgtaa
    55081 cagaagacaa tcgtgtctct cttgatcaaa agctggtcga tacattgtct gatagcgatc
    55141 gaatctatat cgttgcttgc ggaactagtt accatgcggg gttagcaggt aaacagactt
    55201 tggaaaaatt gacacagatt cctgttgagg ttcatttagc cagtgaattt ggctataaca
    55261 cacctcttct ttcaaaaaaa ccattcttta ttttcttgag tcaaagtggc gaaacagcag
    55321 acagccgcca agtgctggta aaaatcaacc gtctcggcta cccttcactt acgattacaa
    55381 atgttgctgg ttctactctt tcgcgggaag catcctttac actgctattg catgctggac
    55441 ccgaaatcgc agtagcatca acgaaagctt atactgcaca aatagctgta ctaactttat
    55501 tagccaaagc tatcggtgat aaaaaagaat ttgaaacttc tcttgtattc gatatcactc
    55561 atgaattgag tttagttgct aatgcgatgg aatcggtcat cgcccaaaaa gattatttag
    55621 aagagttggc agaagtatat ctcagcgata caagaaatgc cttttacatt ggacgtggtg
    55681 ctgactacaa cgtttctctt gaagctgctt tgaaattgaa agaaatcagt tatatccagg
    55741 cagaaggttt tgccgctggc gaattgaaac atggaacgat tgctttgatc gaagaaggaa
    55801 caccagtgat cggaatcatc agtgaagaag tgactggtgc gcatactcga ggaaacctga
    55861 aagaagtcga aagccgtggc gctaaaacac tagttattgt ctcagaggga ttagaaaagg
    55921 aaagtgacca gctggtgcta ccaactgtcc acccttatct aactactcta accacagtcg
    55981 taccgactca attacttgca tattacgcaa cgctattacg gggattcgac gttgacaaac
    56041 cacgtaattt agcgaaatca gtgacagtag aataataaaa gaacaaggac cagtgtcaga
    56101 catctgacac tggtccttgt tcttttattt tttatgaaaa tatctcttaa tccagtccga
    56161 tttcttcttt gactactgcc gcgattttat cgacatagta attaactttc tcatcagtag
    56221 gcgcttctgc catcactcga agtaatggtt ctgtaccgga aggacgaact aggattcttc
    56281 cttctgatcc catttcagct tctgcctctt caatcacttt tttgattgcc ggaacatcca
    56341 tcgcaccgtt tttatcactt acgcggatat tcactaattt ttgcggatag attgttacct
    56401 ctgctgcaag ctctgataat ttcttacctg tctgtttcat gatattcagt aactgaattc
    56461 ctgaaagcat cccatctcca gttgtgttaa aatctaagaa aatcatgtgc ccagactgct
    56521 cgccgccaaa gttataatcg tttttgcgca tttcttctac aacgtaacga tcaccaactt
    56581 gtgtaacgac atctttcaaa ccgattcctt ccaccgcttt gtggaatcct aagttactca
    56641 tgacagtcgt aacgatcgta tcttttttca atcgattttt ttctgccaag tatttagcac
    56701 agataaacat gattttatca ccgtcgacaa tgtttccaag ttcatccacc gcaataatac
    56761 gatcgccatc gccatcgaaa gctagtccag catctgctcc tttttcaacg accatctcag
    56821 ctaaacgttc tggatgggta gagcctacgc cgtcattgat atttaacccg tctggatttg
    56881 tgcccattgt ataaaaatct gtttcaagat cggcaaacaa acgattaaca cttgttgaag
    56941 ttgctccgtt tgctgcatcg acacaaatgg tcaaaccgga aaggtcaccg ttgatcgttt
    57001 gtacaaggaa ttgagaatat ttcaataagc cttctggaaa ttcttctaca gttcccaatc
    57061 cttcagctga tggacgaggc aattcatcca cttcagcatc taataatgct tcaatttcta
    57121 gctcttggtc atctactagt ttaaaaccat ctgcaccgaa aaatttgatt ccattatctt
    57181 gtgctggatt atgagaagca gaaatcatga cccctgcgct tgctttttgc aaacgtgtca
    57241 gataagcaac acctggtgta gagatcacac ctagctggaa cacttcgata cccaccgata
    57301 aaagtcctga aatcagcgct tcttctaaca tttgacctga gattcttgta tcccgcccta
    57361 ctaacacgcg tgggcgtttt ccttcttctt catgctggct taggacataa cctccgcacc
    57421 gtcccagctt aaatgcaagt tctggcgtaa gttccttgtt ggcttcaccg cgtacgccgt
    57481 ctgttccaaa atatttaccc atagttgtaa aattactcct ttaacttaat tttctgagga
    57541 atttgttctg ctcatttgtg ttgattcgct tgtttctgat gtttcttctg tacttgtggt
    57601 cgagctgctt gtcccagatt ccgaactgga agaaatggtt gttgtcgatg gttccgttcc
    57661 tgaatcagca gatgaaatac ttctactcgt acttgtattt tcttgtgtcc gactgctcgt
    57721 cgaagcagag ctactgcttg acgtttgcaa cacgggtgta atctccacac taactgattc
    57781 gggcttgata cttaacccct cttctactgg tatttcaatt ttacgagttg ttcgatgatt
    57841 gatacctgag acattgatcg gtacagcaat actgtcaccg atcgatgcaa gcaaatcgct
    57901 agagccgctt acttctgctt ccagttttga aaaactgaaa tcatagcttt ctaccccggc
    57961 agctggtgtt ccttgctgta tcccataaag tcgtaccgac ttgcttggct tgatcgaatc
    58021 agctctcact gttacttgca ctggatcaga gacgatactt aatacttccc ctgctgcatt
    58081 cagcgcctgt attgggacct cactattaat cccatcatct gtgaccatcg aagcgtccac
    58141 atcagctact actcgtttga tttctgccaa tgttttgtca cctgtagtga tagagacttt
    58201 tttaggacta acggaagtat catcaagttc atatccagat ggtgtgaacg ttgaaggaat
    58261 caacgtttcc acatcgaaat cttttattac ctttttctca accgttactg taatagtcga
    58321 tggttcgatc tttgctgtga cagcactgct caaattttga atttttagcg gaacttcatg
    58381 tgttccttcg cccagcttag tgatatctgc tgtgacgcgg aacatccgtg tatcttcgtt
    58441 tgcctctgca ttcaactgaa ctcggtttgc actggaaagt ttgacagtca ctgcattttc
    58501 atatccatgg atatagtagc gctttgaatc ataagaaatc ttgatcggca cccccgtaac
    58561 tgtttgatta tacgcttcat ttccagaaag cgtatttgga acggactggc catttgcatt
    58621 gaagtacagc gcaatactga acagcaatgc aataaaactg taaagaatgc ttgtccgttt
    58681 ctcttttgag atcatttgcg atcccctccc ttagtcatac cgtctaggaa agattgaatc
    58741 acgtttttct tgttttttgt tttctcttca gcaattagtt ctctacgcaa gattgccaaa
    58801 tattcttgct gggataagcc agccatcagt tcattatcta acgtgatgct gacgtccccg
    58861 gtttcttcag agacaacgat ggtaatcgcg tcactgactt cgcttatacc gacagctgcc
    58921 cggtgtcgag tcccgaattc tttagggatc aacatacttt ctgatagagg aagataggcg
    58981 cttgctactg cgatctttcc ttcttttaca ataactgcac cgtcatgcag cggtgtatta
    59041 ggaataaaga tattgatcag cagctcccca gtgatatctg catctaaagc aatccctgtc
    59101 tcaatatact catctaatcc agtatgacgt tcgatagtga tcaacgcacc gatttttcgc
    59161 ttggacatat attggatggc tttgtcaaaa gacaagatca tcttctcatc ttcctgctgt
    59221 tcgctttttg attgtttgaa aagcgaactg cgtccaagat gttccagtcc tctccggatt
    59281 tccggctgaa aaatcacgat agcagctatc accccgtacg taatgacttg gttcatcagc
    59341 caagacaagg tgtgcagacc aatgatttcc gctacgattc ggataacaat aaaaatagct
    59401 acacctttca gaagctgaat cgcttttgtg cctcgcagca gttggatcag tttgtataca
    59461 agataccaga caaccagaat atcaagtatg ttcaccaaaa aattcagaga gaacaagtca
    59521 ccggaaaaca tttgctgcca atagtccaaa ttaaaaagtt ccccgatttg aaatgacata
    59581 aaaagcctct cttctctttt gagatttcct ctctagtata tcatatgttt taattttaga
    59641 ggctgatatg ctcgtatttt tcttgagaaa aacgcataat tttcaagaaa attgcaagta
    59701 tgcgttttat ttttctctgc aataaatatt tttaaacttc agagaattat tcattatatt
    59761 ttttctgtta tttttccttt ttttcttttg tagcttgttc cattttttta cgtcgttctg
    59821 ctctggcagc tcttctagca gctcttcttt cttctgcttc aaggtccgtc gttttttctt
    59881 ttctagtctt aacgggtaca tcgatagatt cctctggttc aagtcgtttc ttgatttttt
    59941 ctaattctcc cgtcagtgca gcatagttgt aaaaacgtgt tttagcgtcg tggacagtct
    60001 tttcaaataa agcttttgca agatttggat ttgcgttatc tagcgaggaa aagcgaactt
    60061 gtttcctcat gaaatcaggc atagcagaga aatcaggttt tttgtagtcc aaagtcattg
    60121 ggttcttgtt tttttcgacc attgtagggt tatatcgata taatgaccaa taaccagaag
    60181 aaacagcttc ttttgcttct ttaagcgatt ctttcattcc gccaagcaag ccatgtgtga
    60241 tacaaggcgt ataggcaata atcagagaag gaccaggata cttttctgct tcttcaaatg
    60301 ccttgatcgt ttgcatttga ttggcacctg aagcaatttg cgccacataa acatttccat
    60361 atgtcatcgc catcattcct agatctttct ttgaagcata ctttccacta gccgaaaatt
    60421 tagcgattgc cgaagctggt gttgcttttg aggtttgacc gccagtattg gaatagactt
    60481 cgttatccaa aacaaggata ttcacgtctg ctccgctggc aagtacatga tcgatcccgc
    60541 caaaaccaat gtcgtaagcc catccgtctc caccaatcat ccattggctg ttttttacga
    60601 atagatcctt tctcttgtat atatcctcca gttcagggaa aattgatcgt tcgctttcga
    60661 tcgcagcttg cagttttgct gctctttgct gcgttccttc actctcaaac atgtgtacta
    60721 accagtcttc catcaattct cgtagttctt ctgatacttt tggcaatact tctttgattg
    60781 aacttgccaa agcctcgcga cgagtctgtg ccgctagaag cataccataa ccaaattcag
    60841 cattatcttc caataaagaa tttgaccatg caggcccttg accacattca ttggtcgtat
    60901 aaggagaagc aggagaagca ccgccccaaa tagaagaaca gcctgtcgca ttcgcgatca
    60961 tcattcgatc accgaacatt tgtgtcaata attttacata aggtgtttcg ccacagcctg
    61021 agcaagcgcc cgagaattca agaagcggtt gattgaattg tgtagacaag acagtattct
    61081 ttcctttgac tggatttgct ttttgtctta atgtcattgc aaaagcccaa ttcacagctt
    61141 gttctttctg ttcttcataa ggcttcatca ctaaagcttt ttcttttgcg ggacaagcct
    61201 ctacacacag accacaacct gtacagtctt ccactgaaac ttggatacgg tattttacgc
    61261 cgtctgcgcc tcgcatttct ctcacgataa atccttctgg cgcttcttcc atttcctctt
    61321 catctgctaa aaatggacgg atggcagcat gaggacaaac aaaggcacat tcattacaca
    61381 tcgtacagcg atcactaatc cactcaggta cttctaaagc gacgccccgc ttttccatag
    61441 aagccgttcc taaaggcatt tcgccagttg tcattccatt tgctacaagt gtaccaacag
    61501 agagttgatt tccttcctgt gcattcactg gttcaagtat ttcttgtata taaccagtgt
    61561 tccttttatt gattttttca ggcatctcca acgttctcca atggtctggg atcgcgactt
    61621 gatgaagcag ttcggtcgtc cgatcgattg cttgtatgtt tttttctacg atagccattg
    61681 atttatgtcc atagcttttc agggcttctt cttttagcag cggaagcacc tcatcaaaag
    61741 gaatgatgtc agctaattta aaaaaagccg tttccattgc cgtattgatc cggcgtccca
    61801 aaccgacttc ttgggccaat ctcatggcat tgatcgtata gaatctaatg ttattttcag
    61861 ccaagtaacg ttttaatttt aagggcagat gcgtttctaa ttgttcgtcg ctccaaagag
    61921 tattcaataa aaaaatgcca cctggcttca atcctttgac caagtcatag ctatgcaggt
    61981 aagctggtgt gtgacaggca acaaggtccg catgctcgac aagatacgct gaacgtatcg
    62041 gcgtatctcc aaaacgaaga tgagagacag tcagaccgcc agattttttc gaatcataat
    62101 aaaaatagcc ttgcgcatat ttatcggtgt gatctccaat aatcttgata gctgatttat
    62161 ttgctccaac tgttccgtca gatccaaatc cccaaaactt ggcctggaat gtttgcggtt
    62221 ctgtcaaatc cagactttcc tttggttcca aagattgata agtcacatca tccacgatcc
    62281 ctatggtaaa ccgttttttg cgtatgcttg ggtctttttt caattcatca aaaactgctg
    62341 agatttggtc gggagtcaca tctttagacc ccaatccgta gcgaccacca ataacagccg
    62401 ggcgaagttc agaatcatat aacgcacttt gaacgtctaa taagagaggt tctccacctg
    62461 cccctggttc tttacttcga tctagaacag caattgtttt tactgtatga ggcaactttt
    62521 gcacgaatgt ttccactgga aaaggacgat aaagatggat attcaaaaaa cctgttttcc
    62581 gtccgtttgc attcaagtaa tcaatcgttt gttcaatcgt ctgtgcaact gatcccatag
    62641 aaacaatgac ttcttccgca tcgtctgctc cgtaataagt caccaagtca taagaagttc
    62701 ctcgtaatcg gttgatctct ttcatatatt tttgaacgat ttcaggtaat ttatcataat
    62761 aggggttgat cgtttcccgt tgttgaaaat ggatgtctgg attctggttc gtaccactaa
    62821 cagtcggatg attgggattc atccctcgtt tacgaaatga agctaatttc tcctgattga
    62881 tcagaggagc taattcttca tattccaaaa cttcgatttt ttgaatctcg tgactcgtac
    62941 ggaatccatc aaagaaattc aaaaaaggga cttctccttc aatagaagca agatgggcaa
    63001 caggtgctaa atccatcact tcttgtacac tgctttcagc taacatagca aatcctgttt
    63061 gtcttgcagc catcacgtct ccgtgatctc cgaaaatatt caacgcattt gtcgtcactg
    63121 cccgactagc aacgtggaaa acacttggca gtagttcccc agcaatcttg tacatattcg
    63181 gtatcatcaa aagcagccct tgtgaagcgg tatatgtagt tgtcagtgca ccagttttca
    63241 atgatccatg gaccacacca gctgctcctg cttcggattg catttctact acttttactg
    63301 gttgaccgaa tacgttttct tttcctgctg ctgaccattg atctactaat tcagccatgg
    63361 tcgaactggg tgttattgga tatattgcag caatctcggt aaatgcataa gaaatatatg
    63421 cggctgccgt attgccatcc attgttttca tttttcgcat gattcatcat ccttgttcat
    63481 attctctctt attatctgtt ccattataac gtaaaacatg ccattttttt cttttattta
    63541 taaaaatatt ttacatgtat ctatttgaat tggtcatcta atttttagac aaacagtcta
    63601 aatcgttgcc tttttttgtc agacatgtat aaactataag cgaaagaggt gttttaacat
    63661 ggatatatca gtgattgatg caacgaaaac aaatacacaa aaaggaatcc tttatggttc
    63721 atcgaatgct cctaaaaaaa tggtcgaatt catcaactta gcttgtccgt attgccgaca
    63781 atggtttgaa gaatcttatg agctattaga agaagcagtc caaagcgggc aattgcagcg
    63841 agtgatcaaa ttgttcgata aagaaaaaga aagcttacag cgagggaacg tgatgcaccg
    63901 gtatctcaca atcagcgatg gccaaaaagc gatcaaagag atcaaacaaa tattcgatac
    63961 tcaagacgag tggaaacatc tttctctcca agaagtagcg gattttgcag tagataaact
    64021 aaagctgaca gaacaaaaag acgaacagct gagccaagct gtcatcaacg aagctgagca
    64081 agcacatatt cgatttgttc caactgtcat cttgggtaaa gaaatctttg atgaatctat
    64141 ttcaatagag gaattaaaag aattgatcca aacaaaataa aaaaataagg agcgtgggac
    64201 aaaagtaaag aacacttttg tctcacgctc taaacacgta taaacggcgg aagcagaagc
    64261 aactccttct tatttcttgg agttaaacgc ttctgtccca gcctccttat tccgaataaa
    64321 tggtgtccaa aagcttgcat ctgccagatt atctcatttc ttatgacaag ctaagcaaat
    64381 tatctccacc aaataattta ttcatatttt tgacttgcgc atactaacta attcttaaat
    64441 tttcatacaa aatttctcaa tttcccttaa cgagtaatat ttatgtacgg tgattctcac
    64501 tagtatccca atcacttttt aagttatccc gatttcaatt ctacttcaaa aatagttgct
    64561 tttatgtatg ctgttagttg attagatggt catttgttga gctaggtcgt ttattttaaa
    64621 caagttgttt ctttagctta tacctatctc gtctaccaaa cgcaagatgg caaacacaaa
    64681 tgcatacgca tctgtatttg ccactttgat tcgtataaag ctaacaacta ttaactcctc
    64741 tcagctaata gttgctctct tttttattca gattcatttt tgctgatcac agcctagtat
    64801 ttcaatggtt ttgaacacta aaccaaattg ttccattaca acattcactg gctgatgaaa
    64861 tggattagta gatttcgatt attcaaagcg attttctatt ggtggatcgt gttcccaact
    64921 aataagacgg attccaaacc tatcgtacag atcttctaaa ccacttataa attttgcacc
    64981 tatttcaggt gttttattgc gtggatattc tattgttacg ccattaaatg gataccaatt
    65041 aggattaggc tctctttttt tctgtacatc tcccatagaa tagttagcac tttctgggac
    65101 ataatatatt cttcgaccat cctctaattc atctaataag atatttcgtg atcgtctatc
    65161 acttgctaaa tgtagacgcc acgtgcgata ccatgccatg ctacctgcat ctgcagaaaa
    65221 tccatcaaat aatgcaacaa ttgtatcttc tatcacaatc ctttccccat ctacttcata
    65281 ttctaatcta aaatcaaact cccctcgggt aatttctggt cttggtggct taggacccaa
    65341 tattattcta cttaaaaccg cattcgttcc cagatgaaac acagctataa caacaataat
    65401 gattaatatt ttgtttctat ttgataaatt tctcattatt cgcacatcac ctctacattc
    65461 tcacatatta gttcgtaata ctcctttaat atcaaaatat ctactggtcg taataatatt
    65521 cgctatgaat tcctactact cgtataagaa aggccaaatc atcagctttt acttgctgat
    65581 gatttggctt gtttctattt atcgtaattg ttttctaatc accaaaattg ttaacaatcg
    65641 gttcagatat ttcccagttg attaaggtaa ttccgtattg tgtcaaaact tcttgcgctt
    65701 ctccgaaatt aagtacagtt cttatgtatc cactttctcc taatataaag tctacctctc
    65761 ttttaaaacc ttcctcgaga tttctaaggg ctatatgtgg ttttaatgac attgattgta
    65821 cattttcccc catataatat tgcgcacttc ctcgactaaa atagattcct agatcgtccc
    65881 caaaaggtag atcttctcta ccactagcta atctacttct ccatcttcgt tctcttttca
    65941 tagaacctgc gctgaactca cacccgttaa actcagctat gacagtatct tcaattatgt
    66001 taatctgtcc atctagttcg tattcaatcc taaatggaaa ttctccatag gtgatttccg
    66061 gttcgggtat tttaggtgag attaatgcaa agaaaggaaa tattaagctt ggcaacatca
    66121 aaaaaactcc tcctatgatc acaattcccg atgtaatcca aaatataatt tgtacaattc
    66181 tcattgatcc attgtattcc attttattcc ccctattact attcatatcc gcctatctat
    66241 atcgatcttc caatgcagag ataattgctt ccataccttg attttttact gagttaccta
    66301 tgcgagatat cattctccac taaggtgcat catattgtct atctaacatg attaattctc
    66361 caataggtgt atactcctcc aaaaacaaca tttaatgcat ctccctcgac aacataagct
    66421 accatgtttg ctgtatatgt gtttgagtct aattcataag cagataaatt tactgtcgct
    66481 ggattaaata taatcgcatt cgtatttata gctactgaat ttgctgcttc agcaccacct
    66541 tttgaatgtc cgatcattgt gatttcaata gtaggattta cgtggacagc cctcaccgtt
    66601 taccctgacc attatagaaa tcgcttactt ttttgaacta cgcaacaaag gatacaatgc
    66661 tcctaacttc actgtctcca tttttgtcca ctaaggaata taaaaaatag gcattaattc
    66721 atttaattcg gtcacaacac gttacctttt aaatcattat actgtatctc atacattatt
    66781 atcaataaac tattataaaa atgcaaaaaa tgctttatca atctattttg taattggtca
    66841 taatatcatt gaaatcatac cctgtatcga tttttcctat gaatctcgtc tatttgtgat
    66901 tgacgaaaaa gaggcaagac agacactgga atggtaattt tacccactat tttgctccaa
    66961 ctatttgtaa tcgtacccac ttttgaaata ccgagatgat cattttaatt gatatgctga
    67021 ttcccaagca aaaccccaag aaattaaatg ctctatcgca tctagcttct tgggttttgc
    67081 ttatataaat tccaacattt aaatttttta tgctgttcgg tctcagaaat gccgtttttg
    67141 cttatatcac aatatcaaga tgtcaaatta ttcgtgttgc cgatgacttt gattaatttt
    67201 tttcaaattg tttattaaga ctaatgctgt ctttttgcag ataccaatgt tcgaactttt
    67261 taacattatc atatgatcct gcaagtttta acttcttacc attagatgtt atcaaagtta
    67321 taattaacct tgcctttttc ccattaggat cttttatttt atgaattttc tgcaggtaat
    67381 ctctcctaat ggcagctatt tttacctcat cagtgctgat aaaccattga tcatccacat
    67441 acatactgcc tgttatgttg tgtttttcca tttcttccga gaacgaaaag tttaaggctc
    67501 gttcttactt ccaaagttga atgagtccat ggattggact tagtgtgaga attgagaaca
    67561 acaataagaa tccatatcct cccgcaaaca tccctaaggc tatactgtct ctccatccat
    67621 aagtaggatt agaagagaaa agagtcaccg tttgtaataa tatgaaaagg aataatccag
    67681 cacaaacacc aaagataaca actccaaaca ttgaattttt atacaatgac catttattta
    67741 taggcttttc tttcatttta cttcccccta ctgttctgtt cacacggaat gaattcaaag
    67801 ttcataagcc ctcctctttg aacatatgaa tacgacttgc taactatact ttaaaaaagc
    67861 ttgttttaag cggttatagg cgagttgtaa tctctattca tctcattagc aaataacttg
    67921 ccgaaaagtc tactgaaatt aaagcagata gtggaagaat tatttagcca ggatagacac
    67981 tatttgtgaa acacatgtct ttacttttga aattctatga tgaaaagcct tggaatacag
    68041 tagctcttaa ttggtaatac tttttccatt ataaataacc tcttttcatt ttatatttat
    68101 cccctctaaa aataatatct aatctaatat tttaaactta tatttccctc gctgtgctaa
    68161 tagagcatta ctaaataaat agaaactaga aaaaagcatt acgcctgcca gaataaacca
    68221 attatttgt
//
LOCUS       DACEEZ010000006        57991 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20313_2017
            SAMN12639966-rid9879223.denovo.006, whole genome shotgun sequence.
ACCESSION   DACEEZ010000006 DACEEZ010000000
VERSION     DACEEZ010000006.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639966
            Sequence Read Archive: SRR10023158
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 57991)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 57991)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741683.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741683.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:44:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,016
            CDSs (total)                      :: 2,962
            Genes (coding)                    :: 2,889
            CDSs (with protein)               :: 2,889
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 36 of 73
            Pseudo Genes (incomplete)         :: 29 of 73
            Pseudo Genes (internal stop)      :: 18 of 73
            Pseudo Genes (multiple problems)  :: 8 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..57991
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639966-rid9879223.denovo.006"
                     /isolate="E20313_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            83..1081
                     /locus_tag="IWO89_000427"
     CDS             83..1081
                     /locus_tag="IWO89_000427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HAQ0455213.1"
                     /translation="MSNAKFNPQAFQTKPPKQQKEKKQKKRFLFQKKKTEPSDRMIQF
                     SQKSAMSVFSGAFFCLFLLMGVVLLLNFGRVDTLTRLAVKKQVNPETLLTEVNQTLSS
                     TEQLKYEGQHFIERLFTYSPEKRNEWEASVNHYLAQGLEASDLGFVTTATNRVAKDVR
                     FIKLEEIDLTQAMYRLFYEVTFTEGEHWKSSQLSFPVSYQNNVLKVLDGVQFLNLSES
                     KNTNSVNYQENRFYSSGQAVTESEEIKLEAFVKRFFELYVKNDEKLALISNEKGLEGG
                     ELINVQLDHAVKNKEGNYFLQGKYRFYFEKGNEFQSNFSMEVKTSGDSYFVTTLNE"
     gene            1105..1389
                     /locus_tag="IWO89_000428"
     CDS             1105..1389
                     /locus_tag="IWO89_000428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0455214.1"
                     /translation="MKLFDLKSLFDDVSGQVIWLLAIVAIVLMVGAYATQGFARAAFA
                     IIGVFILIMLVLLLSNAVDIGEWLKNIIFIKDSEENVGVTQMIVPWIQLS"
     gene            1470..1868
                     /locus_tag="IWO89_000429"
     CDS             1470..1868
                     /locus_tag="IWO89_000429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HAQ0455215.1"
                     /translation="MEYNYTKEFKHPHKIYGIKGQKIPWISNGIRMEQLVIGGVFLFI
                     LLILFLIAYVNQISFLQTLFRKSWLIMIFGIGVLVWTLFSMKWDNKHFFEYLIGRTSY
                     YQKRKQRFEHEMYVPFFHEKVTYSLHKWKE"
     gene            1878..4421
                     /locus_tag="IWO89_000430"
     CDS             1878..4421
                     /locus_tag="IWO89_000430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion protein"
                     /protein_id="HAQ0455216.1"
                     /translation="MAFLFLLVVFIIGIWQFKKKKTKLPRLEFPIDAIIRNFVLTKND
                     DVWVGYQLSSQVFPLNDIDFFKNYLQDGEGILGHDEYDYHFINIPRQFNLDTHIEETI
                     RDLVKGECADLGKVYFEQAGKILKDEVQLNEYRTYLFVRFTTEMKVSDPIEYFQLLKH
                     VFKKFIYRITGQRVPISSVLPTYETLEKELYTELSNYKPIERLSAQEIGQIYYYFFHR
                     SEEKLPERQLTPMEICEGVISNEAGYIAIEQLNQTHYSTFLSQTDAPLAMYGSSFIQN
                     LQDSLSFPIDTHIRLRFEHSKSDERKINKMAGRIYEQEKEYETVDGILDSDDVLNFGT
                     ERLMDVKDKLKRKEKRLCYSTVTLVVSASSKEELEERVKDIEFAIESTDFKFYRSVAD
                     QLTLFNQCLIGSTQRFRSYEQVATTGFVADYGLDLKKEVGNKKGFPLGRIVIAKKFKS
                     AKEALALSSKLVWYYPNLTKKSIEGAEHTNGNTLIIGPPGKGKSVLVKYIFLWLMFLG
                     QKILYIDPKNETQKFFLKALEKFGHIPEFVAMYERINFISLSSDESCRGMLDPLLFLP
                     KEEAIQTAKNVLGMLGEVDTNRETASHKKTVIQDAIDVVYTMPGKHSLSQVIEEIRKS
                     DKELAKLILGHSVGLGKILIGNEHSTPIRFDSQINVLGTQGIRIPTQKEIDSGRLNSE
                     QLAGMAIMEVIMKFTYIFSTNKEEDAAIIYDEAKGYEDTAQGSYLIDDSLRKGRANNT
                     DIYLVVQAFKDYDQEDKKELISYKFAFRPKQKEAREKLLSFFDMEVNSANLEMMEQLQ
                     TGTCLFQDHLGRNQPIAIDVLFDSWLLAVSSTENQDEKIQAMLENEKQR"
     gene            4443..6785
                     /locus_tag="IWO89_000431"
     CDS             4443..6785
                     /locus_tag="IWO89_000431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0455217.1"
                     /translation="MRKKSLKWWTLLLLGGLFFCLQGATVSADQMNDGAGVTETDRSE
                     GENANDKVEVKEVTTDVDQLYKNYKKNSFELMTNDYESMNLKKKLSYNVAGVMKNFVW
                     SGVKMLGEFNASMVEFLFELDIISAIREPIQNLTQKIADNMLSIAGTIGISFSAIMMA
                     IKYAGEARMKQALRVFLMTILIFTGLSVLKDSGTNKNLFDVLFSLDSTVETAFVKVNP
                     VLTPEEETNYETKETEEVTADKKVETAGKLMAARVFYSNVYEPYLLLNYGTTNVDTIR
                     EKKVKYAGKEYDRINLLLDNDLGTKIGEKIHDKVTEYEADTLKNKNIQYYNSWKNTAF
                     ALFYFVVNLIQTVVYFVLSMFRLIIGTMQLLLVPMLPFLLFLGLFLVTMNVFGNYAKV
                     FGILTFLKGMTGFACILFSSFLSYGFQLSSTQDDPWEKILLIIVYLFAPLGIYLFRGF
                     LGSLFTGRMGVRGALAFAGNPFGAEAKLRREAKQRLKERKEARKAQKQKEKENARKRQ
                     ERQRKRGGGSDVRMPQTAKQATRSAFRRESAQQPRHKAPNRMERMAQKARDLHDQAKQ
                     AEEQSQERMQRTAMEKQARKTADLLQRELQGNPSLRERSRKGASRSPYRNQGSAMVVS
                     LANPKQKHKSYGQKHTPRTELNDRKRRQGQTSRRRKEALTPVTKTSSMKTRTVAGQKH
                     AVQKPLRKGNVPRATGSTTPKSVRRPEGVREHMSAVNRVTAQTKAPSTVSKAYSTMKQ
                     TKSPKQAPKTRSEFRATQAKMKRKREKQPIYRREMKQRKR"
     gene            6801..8975
                     /locus_tag="IWO89_000432"
     CDS             6801..8975
                     /locus_tag="IWO89_000432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF853 family protein"
                     /protein_id="HAQ0455218.1"
                     /translation="MMNAPKRPTHFEQMFSVDRKRKQEKETPKSRFQQLYFIVTLLGV
                     CLYPFFIVGSIPLAIVFMIDKKETRAHIYDFEYESFLKRRSSLFFIFALGFTLLNLFC
                     FIFVLPRGYLSCYLLFPLNLFSHALQFNGQTIFALLLGGTGAGSCLIAYASFIEKRKV
                     VSKKERQTALLNSKEYKERRKNKFQESQKYTAEYQQAYQVAIVIKDVALRNKQLASLA
                     KVILLGTDEYGLPYVMNFSELNQHVIIPATTGSGKTTVIQLLAEHACKFRIPVILVDG
                     KGAGETLQALKEVAEAYNRPIEVFVDGGDLRYNPVKNGNDIAIRDKLTNLAETESVYY
                     STAAKALLQVTIQLLDEFQKSHKIERNLVWLQHYLLPRNVLHLFADRIQLKNPDLFKE
                     KVVVEKKKTTKKKTKKNEAMDKTERDFADVEQLSEERIVEHDRKTVDLETYYLLLKEN
                     ISLMTEIEQTLFKRLFVRYEHKKNPFYLYATSENLQTNINMLLDSELGELFDTSGGKR
                     ELDIQEVVKEGKIVYISLNGLIYSEFIRTLAQMLVGDINFFASEVYKQAGETANRSKV
                     LVIFDEPASYLNDNLIDLVNKGRGAGVHAAYAPQTLADIDRIDPVLRKQLIGNVNTFF
                     IGKTNDPTEIDYWADLMGTEEDIELTEMTTQEAGYSDMGKVDWTGERGTKRDVDRFKF
                     NPNRIRALRTGEFVVYRTAEDVQEPPRVVYIRKPNLEKQYAK"
     gene            8965..9387
                     /locus_tag="IWO89_000433"
     CDS             8965..9387
                     /locus_tag="IWO89_000433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HAQ0455219.1"
                     /translation="MRNKKVIVRILIVSFLLFGCFKLIKTNEESNGKLINSNLEEVLE
                     LQKTNQSFFVYLARNDCKKCQEVESFLERYDKKFSRNVYRIETRKEPRQEELQTFLQR
                     YHVKSVPSFLVIHDRNVKKIERKTFFFEKTLNQFLPNF"
     gene            10268..11179
                     /locus_tag="IWO89_000434"
     CDS             10268..11179
                     /locus_tag="IWO89_000434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0455220.1"
                     /translation="MKYLLHGYFIDYSTRNSSTLAILCLLHELRVVTRKQLYKLLNFD
                     RSISKQGVTNALVRLYEKQLIDRKKYGTEMYYYLTKEGHQSIGGYYTLPKVPEYNLNH
                     HLQINNYLIKGMALAKDHPHLKRIISERRTVYETKDLNPKTKGRKFFVPDFIFRFRDK
                     ENHEINWSFEIELTLKTRRRYREAIFPKYIRELRTRPESHLIYVTPSGAIQEELHAFK
                     RIFFKRETEKDASQQELFERLHIFSALEFEEGLKALLENDPAVNWRTVEREKIYPNGA
                     VQSRLNLFEESTENEEKPKVKFTPEVL"
     gene            11666..11932
                     /locus_tag="IWO89_000435"
     CDS             11666..11932
                     /locus_tag="IWO89_000435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001245899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0455221.1"
                     /translation="MTTKNEQLKEQARKELQERGLIIEGSFEGDFETYIGCYARPINK
                     PTALDPMNEQEVKEQEKHAVNGFPQDFTEWYEWEINNGKLENFL"
     gene            12004..12345
                     /locus_tag="IWO89_000436"
     CDS             12004..12345
                     /locus_tag="IWO89_000436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0455222.1"
                     /translation="MAKNTPYQVGDIFYSSWGYEQTNVTFWQIVKLTEKTAWFQPIQK
                     KTVEHYSSMSGKVVPMKDKFIEYPLAKTKDSIVKKRIDPETYPDMLCGYRSWDTFHRY
                     KNQAVYESSYY"
     gene            12400..12819
                     /gene="ssb"
                     /locus_tag="IWO89_000437"
     CDS             12400..12819
                     /gene="ssb"
                     /locus_tag="IWO89_000437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HAQ0455223.1"
                     /translation="MMNSVNLVGRLTKEVDLKYTPKGNATGTFILAVNRNYTNANGER
                     EADYIRCVIWRKAAETLAKYTGKGSLIGINGRLQTRSYQNQQGQTMYVTEVLVTDFYL
                     LESKEINEQRSKSMANNQNNSSIPGNEIQISDSYLPF"
     gene            12896..13192
                     /locus_tag="IWO89_000438"
     CDS             12896..13192
                     /locus_tag="IWO89_000438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0455224.1"
                     /translation="MKIIDSYDQRTMQYLGSFDYTDENIINYVASLPPYQSVRLVDFA
                     SDELVLTTMGNFLDYVGDKKWLEKILSKLIAKQTGEEPINAIQLFNRYEQENND"
     gene            13207..13638
                     /locus_tag="IWO89_000439"
     CDS             13207..13638
                     /locus_tag="IWO89_000439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0455225.1"
                     /translation="MKRLKRSIFIVLALIDFVGTGCEAIDVQKAPLSEIKQAITKQDE
                     EQPLNTQVEIVNQNKPSFSEEDFSLENGTRQFFSDLDRLNRVGVASALLHKSRMPTEE
                     RDRLYIKPTGWKQKKMKNGDYLYNRCHLIGYQRATSFPISM"
     gene            13820..14125
                     /locus_tag="IWO89_000440"
     CDS             13820..14125
                     /locus_tag="IWO89_000440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0455226.1"
                     /translation="MKLDMPGRYKLLRVRLFLIKLTNLRMYGSITDLYSKDIRLRMQR
                     TLSKSKNVGHEIRDGRLAVYLSRVGKTCLIDSDESGNATKRFTEEIEQLAELNIRYF"
     gene            14529..15017
                     /locus_tag="IWO89_000441"
     CDS             14529..15017
                     /locus_tag="IWO89_000441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HAQ0455227.1"
                     /translation="MKKTIFVLAIFICFGLLLVGYLLFNSNEMRTISTNDLLKKSELK
                     DKKKESLAFIMFTQEECEYCIAINPIIDSVNKETLNKIKKYDISVDFQSKQKCKIKFV
                     PTIVAYSKNKEVGRIDNELDNLYRKKQDGSIVIDKKKTKTLLIDWMKKMDKQGEYLYG
                     ED"
     gene            16351..16653
                     /locus_tag="IWO89_000442"
     CDS             16351..16653
                     /locus_tag="IWO89_000442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAQ0455228.1"
                     /translation="MTKLGITLDDNIYDEKILLSGGEKQKISLARALIKSASVIILDE
                     PFRNLDNETRDNLRDYILNTKKEVCYIIITHDHILDNFLDSNDEILYLNKEYDNNE"
     gene            16646..17674
                     /locus_tag="IWO89_000443"
     CDS             16646..17674
                     /locus_tag="IWO89_000443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HAQ0455229.1"
                     /translation="MNSKKLQMGEIVRGPSQISIDVTYKCNFRCKHCYNASGGDNCFM
                     DNELTDDELLELTKDICNLKPYNICFCGGETLLRKKIVLSCATLLKEAGIPNIAIVTN
                     GSLVTQDVAKELFKAGINRFQVSLDGKNASSHEALRLYPNSFEKAINAIKLFRELSEE
                     VDVAFCPTSFNYNQLREVYQLVNTLKVKSLRIQPFMLSGRGMQNREIVPTVSQYRKLV
                     SDINYIKRNEDYTKIDWGDPIDHIIRHRTLLKDFSNFVSIRSDGGLMASPYLPIQVGN
                     IKCHSLSKYWRAGLYKVFSLDLVQDIATQVSSIQDMSKELKKGHIKWLNEDIEIDILD
                     EYKHDILI"
     gene            17730..17948
                     /locus_tag="IWO89_000444"
     CDS             17730..17948
                     /locus_tag="IWO89_000444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0455230.1"
                     /translation="MISEPQMNNMYESPTIMGAGEAAPRACSAIMVCVLLASAGVGIQ
                     AGAVYTYAVVATVAVAAAAVWKTVGLVG"
     gene            18019..18834
                     /locus_tag="IWO89_000445"
     CDS             18019..18834
                     /locus_tag="IWO89_000445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0455231.1"
                     /translation="MKYNNLKPKLLNENIEYIREENYGYLTIVPKNGTELKDNVFNPT
                     VRYILANCTGKNSVKKIANGLKKKFPSLEYEKFYQDVVGTIKFFKHYNIIDIGDYSME
                     EKKIYKIEQNLELELLNESHYEEIYNFITDHPESMYFHEDIYHQGNSKELEVVLRYNL
                     FSFTSEFYFLRHKERIVGMVSIIVNPFQNLSYSIVNYLICEPAYTTTILTTVLENINE
                     VSILEVKKLVIHLLNSTEDESLIPYLKDSHFFKEAVLSKEYREHDVLRYAYIY"
     gene            18898..19233
                     /locus_tag="IWO89_000446"
     CDS             18898..19233
                     /locus_tag="IWO89_000446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SdpI family protein"
                     /protein_id="HAQ0455232.1"
                     /translation="MILLILSLVLLVVSNLMKNNPPTKINSFYGFRTKKSMKNEKSWH
                     LAQKLLGDFSKKKVIPVFLMGIVFFSIEIYSLAILKNENFFVSVLMGESLILILILCL
                     IVHEVNKQL"
     gene            19657..19920
                     /locus_tag="IWO89_000447"
     CDS             19657..19920
                     /locus_tag="IWO89_000447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0455233.1"
                     /translation="MFIKYGADTVATMFTEEYQVDYYEVIDKCVHFVYEKVWNFMNFA
                     ISEEILRVQENRTGIFTRDRKLNLIAHYIRNRWSSIPNFNYKK"
     gene            20236..20666
                     /locus_tag="IWO89_000448"
                     /pseudo
     CDS             20236..20666
                     /locus_tag="IWO89_000448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347687.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
     gene            20805..21227
                     /locus_tag="IWO89_000449"
     CDS             20805..21227
                     /locus_tag="IWO89_000449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0455234.1"
                     /translation="MVKEEVLCPHQDFLYLIPANQETTQHYFTFLEKQNELVEALMES
                     QETVDYLLSLEFVQHPLEHSKEIYENFELFTSALIYLNHHNYELIPEHWQLLGSSKQQ
                     GKKASVPSLGKKELITIYQQMFEDQPELPIFYQLKKAG"
     gene            21372..21617
                     /locus_tag="IWO89_000450"
                     /pseudo
     CDS             21372..21617
                     /locus_tag="IWO89_000450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347587.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            21726..22682
                     /locus_tag="IWO89_000451"
     CDS             21726..22682
                     /locus_tag="IWO89_000451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0455235.1"
                     /translation="MLILANQASTFVQTQQLRGRGLRLGAQNKVTNIWHLAIGLPGVP
                     LSEQPDLKRMLRRLGNISGLTFESSPKIESGGDRFALPLEYTTTTMETYTQSMFYFSC
                     ERSRLKEQWSTALADGWHLSMPIVVRKVDQVKQKQETSLPIVVKNIEPLSFWSAIFKG
                     NLLEYFQIRRRQKLWEKECARKKSLAICVQTTLQRLGEVSDRKVIVAADQQEFRCYLE
                     NAGYREGQLFTEAMRELLAPLENPRYLIEGKKIVLTVPERFGRNKQIANLFLNELNQS
                     SLPKCQLVYTRNPEGRQKLVTYRMRQVFQTRDEVIIQKNIWT"
     gene            complement(23518..24189)
                     /locus_tag="IWO89_000452"
     CDS             complement(23518..24189)
                     /locus_tag="IWO89_000452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAQ0455236.1"
                     /translation="MTIKTSLQNQAYQSIRQQIIYADLEPGKKVSEKKLEETLSIGRT
                     PIREALIQLRHQGLVDTIPQSGNYISLIDLDLAKNARFVREHLERQIMLECCAKMNNQ
                     KKQVLETIIAEQEKAVSQKNARSFFHNDNLFHKACFEIAEREEVWQWLEDNNTHLERF
                     RWLRVKTEGLKWEKIMEQHHQLFKALTMQDPEEANFLTSLHLHLMLDEQNVVLTTHPK
                     YFKNI"
     gene            24506..26137
                     /locus_tag="IWO89_000453"
     CDS             24506..26137
                     /locus_tag="IWO89_000453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol dehydrogenase family protein"
                     /protein_id="HAQ0455237.1"
                     /translation="MVLIKDWQDSIEGLNKMQVQLPYFNVEAVKQEGQKHPVWLHFGG
                     GNLYRGFHAEIAQCLADQNELESGVVVCETFDEEVVEKAYHDYNNDILEIVMGEDGEL
                     KKRLLAVTADSLYCNPKNITDYQRVVKYFEDPTLQFTTFTITEKGYSLKDSKGQFFSV
                     VEADIKSGPTNPQHTMSIVTSLLYSRFNAGELPIAMVSTDNFSQNGQRFQDSIFMIAE
                     AWQKKGYVSEKFIAYLKDKEKVTFPWSMIDRITPNPSESVKMDLLKAGFEDLDIIHTA
                     KNTNIAAFTNTEKVHYLVIEDSFPNGRPKLEAAGVIMTDRETVDKADIMKVTTCLNPL
                     HTALAITGCLLGYKSIQAEMQDDDLVGLIKGIGYVEGLPVVESPGVIDPKQFIDEVIN
                     KRLPNPYIPDTPQRIATDTSQKVAIRYGETIKKYVEKDGNAENLTFIPIAIAAWLRYL
                     LAVDDQGKNFVPSPDPLLVELQEKFSVLKLGEQDPAKIHCAVQEILENKEIFGLDLYQ
                     VEIGKKIEKIFCEMLVGPQAVRKTINKYITEVGGK"
     gene            26140..27219
                     /gene="uxuA"
                     /locus_tag="IWO89_000454"
     CDS             26140..27219
                     /gene="uxuA"
                     /locus_tag="IWO89_000454"
                     /EC_number="4.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannonate dehydratase"
                     /protein_id="HAQ0455238.1"
                     /translation="MEMTFRWYGANEEKISLQEIRQIPAMKGIVGTLMDIPAGEVWPR
                     ERIKALKDEVEANGLTLKVIESVNVHDDIKIGLPTRDQYIENYKQTIRNLAEFGIEVI
                     CYNFMPVFDWVKSDLDYHLDDGSSTLAYISKNIPTDPEEIIRKVENSTNNFELPGWEP
                     HRLSQIKQLLEAYKEVDEAKLRENFAYFLKAIIPTCEEVGIKMAVHPDDPPYSIFGLP
                     RIVKNRDDLEWLCNVVDSPSNGITLCTGSIAERPDNDVYEILSEFCQRDRIPFAHVRN
                     IKYLSDNGLDFYEAPHKSEYGSLDMYKILKTMYDNGFNGYIRPDHGRMIWGETGRPGY
                     GLYDRALGAVYLNGIWEALEKGASK"
     gene            27293..28720
                     /gene="uxaC"
                     /locus_tag="IWO89_000455"
     CDS             27293..28720
                     /gene="uxaC"
                     /locus_tag="IWO89_000455"
                     /EC_number="5.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronate isomerase"
                     /protein_id="HAQ0455239.1"
                     /translation="MFLDKNFLLENEIAKRLFHKFAEHQPIIDYHCHLDPACLLENKQ
                     YKNLTQVWLNDKGAGDHYKWRLMRANGIEEELISGAGNDYEKFLAFVKTVEKAPGNPI
                     FEWSHLELQRYFDIDLTISEKNAPEIWRRANEKIQTDSYRPKELLKMMDVKAICTTDD
                     PTDDLRYHTELSKQDNSFKVLPTFRPDKVWSIKSSGFSNYILKLSQAAGIPIKNFNGL
                     KQALSQRVDYFHQIGCRLADHGLNTYFYEIADEKILDNLLIKALAGKTDFHLSEIAQF
                     VTGIQLHLMSEYTRYGWTMQMHLNALRNNSSILKEQIGVDVGGDSMGDQASLTQNVVA
                     LFDTAEKSGCLPKTILYSLNPTDWLPLATAMQSFQGGMIQKIQLGCAWWFNDTAEGMR
                     QQLTTMAQQSLLSNFTGMLTDSRSFLSYPRHEYFRRVLCQLLSEWVKAGRIPDDEMYL
                     SQIVENICYRNAYNYFNFFEIESRK"
     gene            28804..29451
                     /locus_tag="IWO89_000456"
     CDS             28804..29451
                     /locus_tag="IWO89_000456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 4-hydroxy-2-oxoglutarate
                     aldolase/2-dehydro-3-deoxy-phosphogluconate aldolase"
                     /protein_id="HAQ0455240.1"
                     /translation="MKKVEILSKLEAAGVIAVVRGKNKDEAIKAINAITAGGIKGIEL
                     TFTVPNAEEIIKEIAKKYADQKDVIIGAGTVLDAFTARIAIMAGAEYIVSPSFDRQTA
                     EICNLYQIPYLPGCMTITEIKEALTAGVEIIKLFPGSAFGPSVVKTYQAPLPYVNIMP
                     TGGVSLDNLEEWFEAGVVAVGVGGNLLAPVEKGDFEEVTRIASKYIDKFYEIKNY"
     gene            29580..30956
                     /locus_tag="IWO89_000457"
     CDS             29580..30956
                     /locus_tag="IWO89_000457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAQ0455241.1"
                     /translation="MERKFGLRDKIGYMFGDFGNDFTFIFASSFLMIFYTKVLGISGA
                     TVGTLFLVARFIDAVTDITMGRIVDGSKPSKTGKFKPWILRMSGPVALASFLMYQTSM
                     ASASMSIKVIYMYVTYILWGSIFYTSINIPYGSMASAITSVAEERTALSTFRSIGATL
                     AGMFIGVLTPIFIYTRDSSGAQIVRGGSTFTIVAGVFSVCAVICYVICYKLTVERVKS
                     DESQLKVSLLKSLKGLAKNRALLSIIAAAIFLLLAQLLTMSMNNYVFPDYYNNAQGIV
                     LMNFINPILMLVLVSPVSLIFSKKYGKKELASVGMFFSAIVYLLLFLIKPSNMYVFLV
                     LTSVGYMGLGIFNTVIWANITDVIDDQEVKSGQREDGTIYAVYSFARKIGQALAGGVG
                     GWTLSIIGYDSLAKVQSGEVLQKLYNSSTLIPAVCFFVVGLILFFVYPLSKEKVLENY
                     NLLNQKHN"
     gene            30967..31761
                     /locus_tag="IWO89_000458"
     CDS             30967..31761
                     /locus_tag="IWO89_000458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HAQ0455242.1"
                     /translation="MNSWIFCIDSDGCVMDTMTYKHNLFFGPIAAEIFDVHHREKFLQ
                     DWNNINLYSETRGINRFLGLVKTLEKNDVPSINRLKHWVSVTKELSNPSLKREIEYSQ
                     SEDLLKALKWSEEVNRQISQAKGLDKPFLSARDTIKALKKYGKIIIVSSANKEAIQEE
                     WERHELLSLVDELCCQDKGKKEDIIRSVIENGCDLDKILMIGDSPGDLEAANKNKVFF
                     YPILVNKEKESWENLRTDILFKCLSGEYIDIEQKYIDTFWKNLTNK"
     gene            31774..33540
                     /locus_tag="IWO89_000459"
     CDS             31774..33540
                     /locus_tag="IWO89_000459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-hexosaminidase"
                     /protein_id="HAQ0455243.1"
                     /translation="MTIKVDLRKKPYHLDEDAISWIEETIDAMTLEEKIGQLFVNMGS
                     SRDKNYLSSMVNQYHIGAVRYNPGTADEVYDQNYILQTESKIPLLIAANTEAGGNGAC
                     TDGTEVGVEVKIGATNDAKYAYELGRVSGIEASAIGCNWSFAPIVDINRNWRNPIISS
                     RSFGQSPDKVLELSLAYMKGIQESGILPAAKHFPGDGIDERDQHLSFSINSLSTEEWD
                     NSFGKVYSGLIEAGLPSIMAGHIHMPAYSKHFNLDLKDEDLLPATLSKELITDLLRGK
                     LGFNGLVVTDASHMLGMTGAMKRSELLPTSIAAGCDLFLFFNDPDEDFNYMMEGYYKG
                     IISEERLRDALRRILGLKAKLGLHKKEKNKILLPKQEAMEKIGLIENKSIFKEVADQA
                     ITLVKDKQDIWPIVPEKYKRILLVDVHGVKGGFGEVVAGNRPQAIDLLKQILSAKGFE
                     VEKYISPEEELKGLSQEEKIKNIAGIYAAKRPIKDLTEKYDLIINIANVNPGTVQRIV
                     WPASKGTPDIPFYIHEIPTIFVSVQCPFHLADVPQVKTYINTYDGKQETLEILVDKLM
                     GLSEFKGISPIDAYCGLKDTFI"
     gene            33568..35376
                     /gene="uidA"
                     /locus_tag="IWO89_000460"
     CDS             33568..35376
                     /gene="uidA"
                     /locus_tag="IWO89_000460"
                     /EC_number="3.2.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003479936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-glucuronidase"
                     /protein_id="HAQ0455244.1"
                     /translation="MLYPIMTETRQVIDLSGLWRFKLDTNDDLQEELALEPMVEKESY
                     PISVPSSYNDLFETENIRNHVGWVWYEKEVILPKKLLEERLVLRFGSATHEAKVFLNG
                     KLINQHKGGFTPFESEINQFIKDGSNRITVAVNNVIDDSTLPVGILKETIQEDGDIKK
                     ENLVNFDFFNYAGINRPVKLYTTPKDYIKDVTINTKIIDSDANVEFNIDTEWTGEILI
                     AIYDEEAQLIAEAKGKEGVIQLKNPTLWEPLNAYLYSMKVSLINENKVIDTYTEEFGI
                     RSIQVSNGKFLINNKPFYFKGFGKHEDTYINGRGLNEAVNIKDFNLMKWIGANSFRTS
                     HYPYSEEIMRLADREGIVVIDEVPAVGLHLNFMATLLDDNVEKHDTWKEIRTENAHKM
                     AIKELIERDKNHPSVVMWSIANEPDSDSEGAKEYFEPLVELTKELDPQKRPVTIVTYL
                     KSTPDICKVGDIVDVLCLNRYYGWYVSGGDLKHAKYLLKKELDGWMERCPDTPIMFTE
                     YGADTVAGLHDTVPTMFTEEYQVGYYEANHEVIDKCDNFVGEQVWNFADFETSQGILR
                     VQGNKKGIFTRDRKPKMIAHYLKKRWSSIPNFSYKK"
     gene            35548..35949
                     /locus_tag="IWO89_000461"
     CDS             35548..35949
                     /locus_tag="IWO89_000461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010818872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HAQ0455245.1"
                     /translation="MQQNYNEKSIQHQFDAYCKKVLRNETKSIRKRNINITDNEEPLT
                     NSNEGKYSQTHFDEQDVYFLFGMEILISDQKLSAAIDHLSEIRRKVILLYYFAGFNDT
                     EIGKILNMSTSGIWYQRKKAVEQLKMGYDLW"
     gene            35943..36188
                     /locus_tag="IWO89_000462"
     CDS             35943..36188
                     /locus_tag="IWO89_000462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002412373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAQ0455246.1"
                     /translation="MVDNPKKRQYPLVPLSIIQSALEEDPISIKMILKHYHSYIAKLC
                     LTNGFNEAGQFITYVDEYMLRQLEIKLIEAILKFKIN"
     gene            complement(36512..36733)
                     /locus_tag="IWO89_000463"
     CDS             complement(36512..36733)
                     /locus_tag="IWO89_000463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0455247.1"
                     /translation="MRNEIANITIIRETLQNHTANEISKHTGLNLSTIKKLKSGERSI
                     EKLNLHDAICLTEFGLKNNRKNVEINIWK"
     gene            complement(36895..36967)
                     /locus_tag="IWO89_000464"
     tRNA            complement(36895..36967)
                     /locus_tag="IWO89_000464"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(36932..36934),aa:Lys,seq:ctt)
     gene            37043..37273
                     /locus_tag="IWO89_000465"
     CDS             37043..37273
                     /locus_tag="IWO89_000465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002358447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAQ0455248.1"
                     /translation="MDFQDYEKIFESYPSILKITDLTKMLNIGKNKAYNLLKDRSIEN
                     IKIGARYKIPKKAVIEYILKIQDEGKNHAIIK"
     gene            37317..38486
                     /locus_tag="IWO89_000466"
     CDS             37317..38486
                     /locus_tag="IWO89_000466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002383715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HAQ0455249.1"
                     /translation="MKLRANIIEKRGTLQVVVSYKDSNGKIRQKWKDTKLKKKGNKKR
                     AEKIRDEFLLELESQLAMECECANPNLLFYDYLLNYLEVSKKQVAFNTYVGYKHYVHN
                     RIYKFFYPKKIKLNRLKPYHLQEFYQYMLNDNCTANTVIHYHAFIRKALQEAVITELI
                     STNVADKVRKPKKKQFVSQVYNQQEILKLLDILSEEKLYLVVLLTAFYGLRRSEVLGL
                     KWSAIDFLNKKIMINHVIIENPENMSQLIKKDQTKNSSSYRTLPLVETIERALVNQAK
                     WQQDNRILLGDDYILEDNEYVFTMEDGRLMKPQYVTHRLSKLIKKNGLKKIRFHDLRH
                     SNASIMLDSGQNMKSIQEWLGHASYSTTANLYTHLTAGVKDRAAEALENAFGFKE"
     gene            39060..39629
                     /locus_tag="IWO89_000467"
     CDS             39060..39629
                     /locus_tag="IWO89_000467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1836 domain-containing protein"
                     /protein_id="HAQ0455250.1"
                     /translation="MEKMRQQLMEWGKELETFRLPHWEELPDLELYMDQVRTLVDRYL
                     SPVIQGEKHPLLTSSMVNNYVKLGLIPAPVKKRYNKEHVAFLLAITTLKQVLTIPEIK
                     EGILFQGKTVGIREAYNLFCDEQEAAVWMVSQLAQGKSHPQKFDQATPVEYIAVRAAT
                     LSFAMKLLAEKTIVLETEYLKEEKTNEKQ"
     gene            39616..40476
                     /locus_tag="IWO89_000468"
     CDS             39616..40476
                     /locus_tag="IWO89_000468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HAQ0455251.1"
                     /translation="MKNNKIAILVDSGTDVPEELIKKYEMYMIPLKIIYKDQVYTDKI
                     DITPEEIYQRLPEEIPGTSLPDGESITEIFEQIKKDGYEKVLAVTISSGLSGTYNVVR
                     LIAQQQEELEAYVLDTKNIGIGAGFSAIQAAKWLEEGVEWDQLISNLNELVERTKVFF
                     NVATLEYLQKGGRIGLVASIVGTALKLNPIISCNEEGIYYTVGKARGRKKSLDKTVAY
                     VKEKIGNAKVFNLAVAHGDAKEEAIEMEARLKREFPQANQIYFGQISPALVVHTGPGL
                     LGVGVQVLEG"
     gene            40666..41370
                     /locus_tag="IWO89_000469"
     CDS             40666..41370
                     /locus_tag="IWO89_000469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAQ0455252.1"
                     /translation="MKKVLVVDDEKPISDIVKFNLAKEGYDVYTAYDGEEALEKVAEV
                     EPDLILLDLMLPKMDGLEVAREVRKTYDMPIIMVTAKDSEIDKVLGLELGADDYVTKP
                     FSNRELVARVKANLRRGATAAKEPEEAAPAELTIGDLTIHPEAYMVTKRGETIELTHR
                     EFELLFYLAKHLGQVMTREHLLQTVWGYDYFGDVRTVDVTVRRLREKIEDNPSHPNYL
                     VTRRGVGYYLRNPEQE"
     gene            41375..43210
                     /gene="walK"
                     /locus_tag="IWO89_000470"
     CDS             41375..43210
                     /gene="walK"
                     /locus_tag="IWO89_000470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall metabolism sensor histidine kinase
                     WalK"
                     /protein_id="HAQ0455253.1"
                     /translation="MKRKVRFFRSVNFKIAITFILILLISIEIIGAYFIRGLERSTIN
                     TFIKDMNQTVESLATTISPELNRKDNADDEEVNANIKRFIENSATSDIIEIRVVDEKG
                     IIRGTTDVNEQSAVGKKNDYVDINDFTTKRYVALDNDDKRVNINVQPILSPTGDAVIG
                     ALYVKSNIEQKYSEINNTAVIFFTASLIAAFISMIVSVLVARSITQPIGEMREQAIRI
                     ARGDYSRKVKVHGQDELGQLADTFNQLAERIEEAQDTMEAERNRLDSVLSHMTDGVIA
                     TDRRGKVITINDMAVSLLDIKNEEAIGQSILTLLDIEEEYTLRKLLEDPNEMVLDRST
                     STLESDQMILRVDFSMIRRESGFISGLVAVLHDVTEQEKTERERREFVSNVSHELRTP
                     LTSMRSYIEALSEGAWKDEEVAPNFLKVTLDETDRMIRMINDLLNLSRMDTGNTQLQL
                     EYVNFNELVNFVLDRFDMMVGNQEKNYKIRREFTQRDLWVEVDTDKIIQVVDNIMNNA
                     IKYSPDGGTITCRLLETHNNVILSITDQGLGIPKKDLNRVFERFYRVDKARARAQGGT
                     GLGLAISREVIKAHRGAIWAESKEGKGSTFYISLPYEPYEEEWWE"
     gene            43207..44523
                     /locus_tag="IWO89_000471"
     CDS             43207..44523
                     /locus_tag="IWO89_000471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0455254.1"
                     /translation="MRISEKVVRIGLILMVALSIYFSYAIWLSPAGKSSINVDETNTQ
                     MIESQSNRKTSEVFLPLHVTWHHSGMIQETNSENLVYRLQLIIDKARFGKLSEVVSGD
                     KSQFEQVKNLNSGFELAYNAPFLLREYKEAFDLDLDVSNLAKESENMYFTRIQFDQGN
                     KKIRFLNYSNHLVYEANISLNWTDINKELTAKDTKWTEMTTEPSTIDTQYATKHSIKL
                     KKYSYILSQQPYTLFRNAFFQKPDSVKNNDESNELIFYGGNETMSIHSETQVVDFRGE
                     LPEKKENASVFSESFPYISKLGSSLGNLRYFDRSDNTIDYRIFVEGFPVFGTDSRGQI
                     GVEVGGETNGSVQVNIQTSLNTIQVPIPSDEEVELASTNEIIQQLKAKGADSNKIGSV
                     IVGYTWQNVKETNRVVDLLPEWYVKYKDNWYSANDLLEKLPGLEAN"
     gene            44524..45393
                     /locus_tag="IWO89_000472"
     CDS             44524..45393
                     /locus_tag="IWO89_000472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0455255.1"
                     /translation="MDFKKIEWIFFVAFLGLNVFLFSSYHDAVYQENNVIRSNQTESI
                     QQRLASDDITYTGKISSENKQGYYLSAEQTNLSTSLANYRKAGKSGLLASGVFLDGDV
                     LTKSLSSSDAEKNVIDPDKISSSLEDFLSNKDNILFGNDYVYTKNFSHLEEEPLEIQA
                     SQEYEGIPFNDDTAKIVLSLEKNDNDYSITKYMQTHLSDIEQLREKTELYTEEEAINT
                     LYINNKIPRGSKILWRQLAYSCILKVREKNVYVPVWHVAIETSDKVVQIESVNAFSNT
                     IVTNNTVPKVEDQ"
     gene            45448..46257
                     /locus_tag="IWO89_000473"
     CDS             45448..46257
                     /locus_tag="IWO89_000473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HAQ0455256.1"
                     /translation="MAEEKPAFKISILASGSSGNSLYIESEKKRLLVDAGLSGKKITS
                     LMAQIDRTPDQLDGILVTHEHRDHIHGVGVLARKYHLDVFANQQTWEAMEPLIGNVPV
                     EQKHLFEMGKVRTFGDIDIESFGVSHDAAAPQFYRFHKENRSFVMLTDTGYCSDYMRG
                     VIENADGYLMESNHDLEMLRMGPYPWNLKQRILGDRGHLSNEDGALVMTDVIGNRTKR
                     IYLGHLSKENNMKELAHLTMENVLKEKDFGVGHDFEIYDTDPDEATELFSI"
     gene            complement(46360..46989)
                     /locus_tag="IWO89_000474"
     CDS             complement(46360..46989)
                     /locus_tag="IWO89_000474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-binding protein"
                     /protein_id="HAQ0455257.1"
                     /translation="MKIALIGATGHAGSFILDEALSRDLDVTAIVRHPEKLPSDVPFI
                     QKDLFELTTTDLASFDVVVDAFNAPKGKEEMHQTSLSHLIEMLEGTDTRLIVVGGASS
                     LFLDTEKTTRMIDQVPKNAPFYPTAFNMYEGLLKLKQSKNLHWTYISPASLFDPHGKR
                     TGTYLLSDDYLQRNAASDSTISMADYAIALVDEILDEKHEKQHISVVSR"
     gene            47159..48451
                     /locus_tag="IWO89_000475"
     CDS             47159..48451
                     /locus_tag="IWO89_000475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAQ0455258.1"
                     /translation="MKEWLDRAFLPMITSVVNLRPLTALRNGMTYTIPIIIVGSLFFL
                     FAHFPVDYIAQWIDKTGFSMYFTQVYQATFDVIAFWIVFAIAYAYAKEEDIDRLPVGI
                     TALSAYLLLIQPLNTQIDTVWTGAEGILGAVTTGLIIGWGYARLVRLAKGWKQREKIP
                     ENVWQSFTSLIPIIVIVTFTLIGSAVFQHLFRMTVVQGVWTYVQLPIQSFIDTLFGVL
                     LLGFFVPFLWLFGLHGSAMVNGLVSPVLQANSLANAEILASGQELTVANGGHIVTQQF
                     LDQFMTVTGAGLTLGAVFFMTVFAKSKKYRELGNLALLPAFFNINESIIFSTPIVMNP
                     MMAVPFIFAPVVSGLITYSALYFGFVPLFTAVQVPWTTPPILSGFLVGGVPAAILQGI
                     VLSISFFIYFPFLKKIDSANCKKERQTKNDGAAEKMND"
     gene            complement(48527..49189)
                     /locus_tag="IWO89_000476"
     CDS             complement(48527..49189)
                     /locus_tag="IWO89_000476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HAQ0455259.1"
                     /translation="MSLKKYSFLSIFCYGLIFISPLILASIGLVKSTSELITATAVMY
                     ILGAVVLAIFYFKQREPLAIESAVKKASAPKIVIYGLVGIFVALILQSIAVMLESVLF
                     GKATPSENTQNIIQMIMEAPAFILATTVAGPIMEEFVFRRSILGIISRYSNFWVGAVI
                     SSLLFAFAHNDGHLLIYFFLGFFFSLEYKATGRIWTSMITHVGMNTLVVLVQLAVQKG
                     LV"
     gene            49414..49698
                     /locus_tag="IWO89_000477"
     CDS             49414..49698
                     /locus_tag="IWO89_000477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HAQ0455260.1"
                     /translation="MLKPLGDRVIIEVAKEEEKTVGGIVLASAAKEKPQTGTVVAVGE
                     GRLLENGEKVPAAVKAGDQVMFEKYAGTEVKYEGKEYLIVAGKDIMAIVE"
     gene            49749..51374
                     /gene="groL"
                     /locus_tag="IWO89_000478"
     CDS             49749..51374
                     /gene="groL"
                     /locus_tag="IWO89_000478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736685.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HAQ0455261.1"
                     /translation="MAKELKFAEDARAAMLRGVDKLADTVKVTLGPKGRNVVLEKSYG
                     SPLITNDGVTIAKEIELEDHFENMGAKLVSEVASKTNDIAGDGTTTATVLTQAIVREG
                     LKNVTAGANPLGIRRGIELATKAAVEELHNISTVVDSKEAIAQVAAVSSGSDKVGHLI
                     ADAMEKVGNDGVITIEESKGIETELDVVEGMQFDRGYLSQYMVTDNDKMEAVLENPYI
                     LITDKKISNIQDILPLLEQILQQSRPLLIIADDVDGEALPTLVLNKIRGTFNVVAVKA
                     PGFGDRRKAMLEDIAILTGGTVITDDLGLELKDATIENLGNASKVVVDKDNTTIVEGS
                     GEKEAIEARVQLIKNQIAETTSDFDREKLQERLAKLAGGVAVVKVGAATETELKELKL
                     RIEDALNATRAAVEEGMVSGGGTALVNVISKVSAVEAEGDVATGIKIVVRALEEPIRQ
                     IAENAGYEGSVIVDKLKNVELGTGFNAATGEWVNMVEAGIVDPTKVTRSALQNAASVS
                     ALLLTTEAVVADKPEPAAPAAPAMDPSMMGGMM"
     gene            complement(51463..52611)
                     /locus_tag="IWO89_000479"
     CDS             complement(51463..52611)
                     /locus_tag="IWO89_000479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HAQ0455262.1"
                     /translation="MLKVWVEQTKDGQFKFIERYVDPYTEKTRKKSTTLTSNSPQAWK
                     KAQKILDKKIKEALEDYNKSDITFGELYKEWYEYYKQHVKRTSYLKVPMMMKHVSKHI
                     SDDTIVRNIDETLINKIIEDMYTFGDLSLNYTKQTKTTLSVMLNYAIDRKYIQRNPAL
                     AVKIHPKKVEEEKRRLSMNKKYLEKEEIDQILKQLYSNPRRKLHGIIAEFLYLTGLRY
                     GELLALQMKDYEDGKISINGTLDYTSVKMDNAIKTTPKNTYSQREVQLPNRAKELIES
                     VIADNILAGRPTDPDQYIFISTSGTPLALHSFNAILHKVEEELELEKSLSSHIFRHSH
                     VSLLSELGVPLKAIMERVGHSDANTTLSIYNHVTKRAKQQVIDKLNSL"
     gene            complement(52713..53297)
                     /locus_tag="IWO89_000480"
     CDS             complement(52713..53297)
                     /locus_tag="IWO89_000480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4352 domain-containing protein"
                     /protein_id="HAQ0455263.1"
                     /translation="MAKKVMGQDGKMYKVSKPFYKKVWFWVLAVILIIIIGSALNGGS
                     DSNKASDNGGEKVTKSSTSASSSKEEKSDTFYKIGDTVKVGDAEYTLNSVELTDERNQ
                     FEENQPAQVVKITYTVKNDGDSDIPVGTDVEVYGPDDKKSETYANENTMGSVAPGKQM
                     DVTAHFTLNQKGEIEIHFSPLVSFEKAAIFKATV"
     gene            complement(53424..53855)
                     /locus_tag="IWO89_000481"
     CDS             complement(53424..53855)
                     /locus_tag="IWO89_000481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ImmA/IrrE family metallo-endopeptidase"
                     /protein_id="HAQ0455264.1"
                     /translation="MYLKLKEMLSEYNLKVVYMEMKEPGFYYPKPRIIFLNENLYGET
                     AEAFHLSHELAHFTASHFEYSVLYDTSTTFHSKFETEADKIAILILLNIYIENELTDE
                     SQFNLEKFMEYYSIQNKLRYTCYAVCQCYFKKKYSYARQYV"
     gene            complement(53873..54193)
                     /locus_tag="IWO89_000482"
     CDS             complement(53873..54193)
                     /locus_tag="IWO89_000482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAQ0455265.1"
                     /translation="MNTYEIIKELAKEKGLSIRQLEMNFGYSNGYLGSWKRQTPNSIE
                     LARLADYFGVSVDYLLGREERETPKHVDLSENDTVFSFDGKEISKETMRKAIAIAKAL
                     EENE"
     gene            54931..55188
                     /locus_tag="IWO89_000483"
     CDS             54931..55188
                     /locus_tag="IWO89_000483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomannomutase"
                     /protein_id="HAQ0455266.1"
                     /translation="MNKEMSQTIKVQKMIDDLTHGIDSQADKIIKELQGQKVKDAKML
                     LKTINFEMNPTKRKLADVLEEKLASAINEQELLFETDTFNS"
     gene            complement(55159..55467)
                     /locus_tag="IWO89_000484"
     CDS             complement(55159..55467)
                     /locus_tag="IWO89_000484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0455267.1"
                     /translation="MKPEFVITMLDGEKIKIFADTLVVGYDDASNIEEKDGMFYSSQI
                     YVNTLQGDIGVSTLATSRPLVGLIGFLSKATFFSLGDDIDSDETVIYKTSAVKSIGFK
                     "
     gene            55545..56291
                     /locus_tag="IWO89_000485"
     CDS             55545..56291
                     /locus_tag="IWO89_000485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage antirepressor"
                     /protein_id="HAQ0455268.1"
                     /translation="MNTPQIFNFEQNEVRTILVNDEPYFVGKDVADVLGYSNPQKAIR
                     DHVDLEDKTQNDSFTVNGTAVVLINESGLYSLILKSKLPSAKKFKRWVTSEVLPTIRK
                     TGSYSNVPQSFAQALRLAADLEEKNQLLEQQIAEYEPKISYLDTILSSTDTVATSQIA
                     ADYGMSAIALNKLLNELGVQHKVSGQWILYRKHMNQGYTKSHTSEIPKADGGTKVVMN
                     TKWTQKGRVFIYNLLIAEGYYPQMDLEEIG"
     gene            56306..56509
                     /locus_tag="IWO89_000486"
     CDS             56306..56509
                     /locus_tag="IWO89_000486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAQ0455269.1"
                     /translation="MTDIAEITQRDREKIKEYVESSKFLTYTMLAERFGISKSYLSLI
                     LNGKKTSAEANRIIDSIITMYEL"
     gene            56614..56913
                     /locus_tag="IWO89_000487"
     CDS             56614..56913
                     /locus_tag="IWO89_000487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0455270.1"
                     /translation="MKKPTLSELIEAAEKAVKPDDWYRQSLILEKFHGMSKTTLVEYC
                     KEMETIPEFAEGILRPGHSTTFIHYHTFIWFLKWKDANKYRVKSLSPSDVLKEAS"
     gene            56913..57095
                     /locus_tag="IWO89_000488"
     CDS             56913..57095
                     /locus_tag="IWO89_000488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0455271.1"
                     /translation="MGKFNRALVFSAPLIIYALGLWGSRQALIGTIVYMVWIFMGLDE
                     AEHRAKKASREGLTKI"
     gene            complement(57101..57577)
                     /locus_tag="IWO89_000489"
     CDS             complement(57101..57577)
                     /locus_tag="IWO89_000489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0455272.1"
                     /translation="MRKKLNYILTATLTIYIILFITLSFLSSIKKIPFITLNKNSILK
                     EFNNTDYIAFLKLFSVCVLISCVIIFIVAKTCWTMLNKNQRDVENIAIFLIKITSSIS
                     IIIPMTVTFSKEQFNVAATYLSFIALFSFVLPKRNKSNFDNKELDEVRNSSPKEQK"
     gene            57630..>57991
                     /locus_tag="IWO89_000490"
     CDS             57630..>57991
                     /locus_tag="IWO89_000490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rha family transcriptional regulator"
                     /protein_id="HAQ0455273.1"
                     /translation="MQELVILKNKEAVTTSLQVAESFKKKHKHVLEAIESIKRSVENS
                     ANVEDGSNFGQMFVEGNEPDSYGRSRRVYFMNRDGFSLLAMGFTGSKAINFKLKFIEA
                     FNEMEDVIRKNTVPQTIE"
BASE COUNT        20264 a         8668 c        11547 g        17512 t
ORIGIN      
        1 gagggggcaa gtgtggttgt ccacgaagta gtgattgtgg acaaaatacc gaaattattg
       61 ttagaacggt atggcatttc tcatgagtaa tgcaaaattt aatccacaag cgttccaaac
      121 taagccaccg aaacaacaga aggaaaagaa acaaaagaaa cgttttttgt tccaaaaaaa
      181 gaaaacagaa ccgtcagatc gaatgatcca attttcgcaa aaaagtgcca tgagtgtatt
      241 ttcaggcgct tttttttgtt tgtttctgtt aatgggtgtc gtgctcctcc taaactttgg
      301 acgagtggat acattaacgc gtttagcagt gaaaaaacaa gtcaatccag aaacattgtt
      361 aactgaagtc aaccaaacct tgtcttctac ggaacaattg aagtacgagg gacaacattt
      421 tattgaaagg ttatttactt attcgccaga aaaacgaaac gaatgggagg cttccgtcaa
      481 tcattattta gcacaaggat tggaagcgtc tgatctaggc tttgtaacga cggctaccaa
      541 tcgagtagcg aaagatgttc gctttattaa actagaagaa atcgatttga cacaagcaat
      601 gtatcgccta ttttatgagg ttacttttac agaaggagaa cattggaaaa gcagtcaact
      661 ttcttttcca gtcagttatc aaaacaatgt tttaaaagta cttgatggcg ttcaattttt
      721 gaatctttct gagagtaaga ataccaattc tgtgaactat caggaaaatc gattttattc
      781 ttctgggcaa gcggtcactg aaagcgaaga aatcaagctg gaagcatttg tgaaacggtt
      841 ttttgagtta tatgtcaaaa atgatgaaaa attggcattg atttctaacg aaaaagggtt
      901 agaaggcggc gaattgatca atgtacaact ggatcatgca gtaaaaaata aagaaggaaa
      961 ctactttttg caagggaagt atcgtttcta ttttgagaaa ggaaacgagt tccaatcaaa
     1021 cttttcaatg gaagtaaaaa caagtggcga tagttatttt gttactacgt taaatgaata
     1081 aaatgaactt agaggagaat ggaaatgaaa ctttttgatc ttaaatcgtt atttgatgat
     1141 gtatctggac aagtcatttg gctattggca atcgttgcga ttgttctaat ggttggtgct
     1201 tatgcgaccc aaggctttgc gcgggcagcg tttgccatta ttggcgtctt tattttgata
     1261 atgctcgtct tattgttatc gaatgccgtt gatattggtg aatggctaaa gaacattatc
     1321 tttattaagg atagtgaaga gaacgtgggg gtaacgcaaa tgattgtgcc ctggatacaa
     1381 ttatcataag aaaaagacaa actgcaaaag cgatcagaag attagaactt ctggtcgctt
     1441 ctttttttgt aaaaggaggt agagtgaaca tggaatacaa ctatacaaag gagtttaagc
     1501 accctcacaa aatttacggg attaaaggac aaaaaattcc ctggatttct aatggcattc
     1561 gcatggaaca attagtcata ggtggggtat ttctcttcat tcttttgatc ctatttttga
     1621 ttgcgtatgt gaatcaaatt agtttcttgc agacactgtt tcgaaagtcc tggttgatta
     1681 tgatttttgg gattggtgtt ctcgtttgga cattgttttc aatgaaatgg gacaataaac
     1741 actttttcga gtatttaatt gggcgaacgt cctattatca gaaacggaaa caacgctttg
     1801 aacatgaaat gtatgtgccg tttttccatg aaaaagtgac gtattcccta cacaaatgga
     1861 aggagtgagt cgagtaaatg gcgtttttgt ttcttttggt cgtctttatt atcgggattt
     1921 ggcagttcaa aaagaaaaaa acgaagcttc ctcgattaga atttccgata gacgccatta
     1981 tccgaaattt tgttttgacc aaaaatgatg atgtgtgggt aggctatcaa ctttcttccc
     2041 aagtatttcc tttaaatgac attgattttt tcaagaacta tcttcaagat ggcgaaggca
     2101 tacttggaca tgatgaatat gactatcatt ttatcaatat tccacgtcaa tttaacctag
     2161 atacgcacat tgaagaaacc attcgagatt tggtaaaggg agaatgcgca gatcttggga
     2221 aagtgtactt tgaacaggca ggaaagattt taaaagacga agtgcaatta aacgagtatc
     2281 gaacgtatct gtttgtgcgt ttcactacag aaatgaaagt gtctgatccg atcgaatatt
     2341 ttcaattgct taagcatgtt tttaaaaagt ttatctatcg tattacggga caacgagttc
     2401 ctataagtag tgtgttacca acgtatgaaa cattagaaaa ggagctctat acggaattgt
     2461 ccaattataa gccgatagag cgcttatctg cccaagagat tggacaaatt tattattatt
     2521 ttttccatcg atcagaagaa aaattgcctg aaagacagct tacgccaatg gaaatttgcg
     2581 agggcgtcat ttcgaatgaa gcaggataca ttgcgattga acagttgaat caaacgcatt
     2641 attcgacgtt tttaagtcaa acggacgctc ctttagccat gtatgggagt agctttattc
     2701 aaaatcttca ggatagctta tcttttccaa ttgatacgca tattcgactt cgatttgagc
     2761 attcaaaatc tgatgaacga aaaattaata aaatggctgg acggatttat gaacaagaaa
     2821 aagaatatga gaccgttgac ggcattttag attcagacga tgtattaaat tttggtacag
     2881 aacggttaat ggatgtaaaa gataagctga agcgcaaaga aaaacgattg tgttattcta
     2941 ccgtcacgct ggttgtatct gcttcttcaa aagaagaatt ggaagaacga gtcaaagata
     3001 ttgaatttgc gattgagtct acagatttca aattttatcg atcggtagcg gatcagctaa
     3061 cgttgtttaa tcaatgctta attggaagta cgcaacgttt tcgtagttac gagcaggtcg
     3121 ctacgacagg ttttgtggca gattatggct tggacttaaa gaaagaagta ggaaataaga
     3181 aaggatttcc tttaggacgg attgttattg caaaaaagtt taaatctgcg aaagaagcgt
     3241 tagcgctatc tagtaaacta gtatggtatt atcccaattt aacaaagaaa agtattgaag
     3301 gcgcggaaca taccaatgga aatacgttga ttattggtcc accaggtaaa ggaaaatcgg
     3361 tccttgtaaa atatattttt ttatggctga tgtttttagg tcaaaaaatt ctttatattg
     3421 atccgaaaaa tgaaacccaa aagttctttt taaaggcact tgaaaaattc ggacacatac
     3481 cagagtttgt ggctatgtat gaacgaatta attttatttc gttatcttca gatgaaagct
     3541 gtcgagggat gcttgatcct ctattgtttt taccaaaaga agaggcaatt caaaccgcta
     3601 aaaatgtgtt aggaatgtta ggagaagtag ataccaatcg agaaacagcg agccacaaga
     3661 aaacggtgat ccaagacgcc atcgatgtag tttacaccat gccaggtaag cattcgttaa
     3721 gtcaagttat cgaagaaatt cgaaaaagtg acaaagaact agctaaattg attttagggc
     3781 atagcgttgg tttaggaaag attctcattg ggaatgagca cagtacgccg atacgttttg
     3841 attcacaaat caacgtgtta gggacacaag gaatacgcat tccgacacaa aaagagattg
     3901 attcgggtcg attaaatagc gaacaacttg caggaatggc aattatggaa gtcattatga
     3961 agtttacgta tatcttttct acaaacaaag aagaagacgc tgcgattatt tatgacgagg
     4021 caaaagggta tgaagatacc gcacaaggaa gctatttgat tgatgatagc ttgcgaaaag
     4081 gacgtgcgaa taatacggat atttaccttg ttgtgcaagc gtttaaagat tacgatcaag
     4141 aagataaaaa agagttaatt tcttacaaat ttgcgtttcg tccgaaacaa aaagaagcac
     4201 gagagaaact attgtccttc tttgatatgg aagtgaattc agccaattta gaaatgatgg
     4261 aacaattaca aacaggaacc tgtttattcc aggatcattt gggaagaaat caaccgattg
     4321 ccattgatgt gttgtttgat agttggttat tggctgtttc ttctactgaa aatcaagatg
     4381 aaaaaattca agcaatgtta gaaaatgaaa aacaacgata aaacaaaaaa gaggtggttt
     4441 ttttgcgaaa aaaatctctg aaatggtgga cgctcctttt attagggggt ctttttttct
     4501 gtctacaagg ggctactgtt tctgccgatc aaatgaatga tggtgcgggt gtcactgaaa
     4561 ccgatcgttc tgaaggagag aatgccaacg ataaagtaga agtaaaagaa gtaacgactg
     4621 atgtggatca attgtataaa aattacaaaa agaattcgtt tgaattgatg accaatgact
     4681 atgagtcaat gaaccttaaa aaaaagctat cgtacaatgt tgcaggagtg atgaaaaatt
     4741 ttgtttggag tggcgtaaaa atgttaggag aattcaatgc ttcaatggtt gaatttctct
     4801 ttgaattaga tattatttcc gccattcgag aacccattca aaacttaaca caaaaaattg
     4861 cggacaatat gttaagtatt gcaggaacta ttggaatcag tttttcggca attatgatgg
     4921 caatcaaata tgctggagag gctcgaatga aacaagcact ccgtgtcttt cttatgacga
     4981 tccttatttt tactgggtta agcgtattaa aagattctgg gacaaataag aatctttttg
     5041 atgttctttt ttctttagat tctacagtgg aaacggcgtt cgtgaaggtc aatccagtgt
     5101 taacacctga agaagaaaca aattatgaaa caaaagaaac agaagaggta acagcggata
     5161 aaaaagtgga aacggcaggc aaattgatgg cggctcgggt cttttatagt aatgtttatg
     5221 aaccatatct gttgttaaat tatggcacaa ccaatgtaga cacaattcgt gagaagaaag
     5281 tcaaatatgc tggaaaagaa tatgatcgaa tcaatctttt attggacaat gatttaggta
     5341 caaaaatagg ggagaaaatt catgacaaag tcacagagta tgaagcagat acactgaaaa
     5401 acaagaacat acaatattac aatagttgga aaaatacagc atttgccttg ttttattttg
     5461 tcgtgaatct gattcaaacc gttgtttatt ttgtcttaag tatgtttcga ttgattattg
     5521 gaactatgca actattgtta gtaccgatgt tgccgttctt acttttttta ggattgtttc
     5581 ttgtcacaat gaatgtgttt ggtaactatg ccaaagtttt tggtattttg acgtttttaa
     5641 aaggaatgac aggttttgca tgtatccttt tttcttcttt tcttagttac gggttccaat
     5701 taagtagtac acaagacgat ccatgggaaa aaattctctt gatcattgtt tatttgtttg
     5761 cgccgttggg aatttatttg ttccgaggct ttttaggcag tctatttact ggaagaatgg
     5821 gtgttcgagg agcgttagct tttgcgggaa atccatttgg ggcagaagca aaattgagaa
     5881 gggaagccaa acaacggttg aaagaacgca aagaagcgag aaaagcacag aaacaaaaag
     5941 aaaaagaaaa cgcacggaag agacaagaac gtcaacgaaa acgtgggggt ggctcagacg
     6001 ttcgtatgcc acaaacggcg aaacaagcca cacgaagtgc ttttcggcgt gaatccgcac
     6061 aacaacctcg tcataaagca ccaaatcgta tggaacgtat ggcacaaaaa gcaagagact
     6121 tgcatgatca agcgaaacaa gcagaagagc aaagtcaaga acgtatgcaa cgaacagcaa
     6181 tggagaaaca ggcaagaaaa acagccgatt tattacaacg agaattgcaa ggcaatccta
     6241 gtctaagaga aagaagtaga aaaggtgcgt ctcgtagtcc gtatcgcaat cagggttctg
     6301 caatggtggt gtcattagcg aatccaaagc aaaaacacaa gtcttatggc cagaagcata
     6361 cgcctagaac agaattgaac gatcgaaaac gtcgtcaggg acaaacaagt cgtcgtcgga
     6421 aggaggcact tactcccgta acaaaaacaa gttcgatgaa aacccggaca gttgcaggac
     6481 aaaaacatgc ggtacaaaaa ccactaagaa aagggaatgt accacgagca acaggttcta
     6541 ccacaccgaa atctgttcga cgtccagagg gggttcggga acatatgtca gcagtcaatc
     6601 gagtaacagc acaaaccaaa gcgccttcaa ccgtatctaa agcatactca accatgaaac
     6661 aaacaaaatc accaaaacaa gcaccgaaga cacggagtga gtttcgagca actcaagcaa
     6721 aaatgaaacg aaaacgagaa aaacaaccga tttatcgaag agaaatgaaa caacgaaaac
     6781 gataggagga aatcgatagt atgatgaatg caccaaaacg accgacacat tttgagcaaa
     6841 tgttttctgt cgatcgcaaa agaaaacaag aaaaagaaac gccaaaaagt cggtttcaac
     6901 aactttattt tatcgtcact ttattaggtg tttgtctata tccatttttt attgtaggca
     6961 gtattccttt agcgattgtc tttatgatag ataaaaaaga gacacgtgca catatttatg
     7021 actttgagta tgagagcttc ttaaaacgaa gaagctctct tttttttatt tttgcgttag
     7081 gttttacgct attaaatcta ttttgtttta tttttgttct tcctagaggg tatttgtcgt
     7141 gttacttatt atttccatta aacttgtttt ctcatgcgtt gcagtttaat ggacaaacga
     7201 tttttgccct ccttttaggt gggacaggtg caggaagttg tttgattgcg tatgcgtctt
     7261 ttattgaaaa aagaaaagtt gtttcaaaga aagaacggca aacagctttg ctaaatagta
     7321 aagaatacaa ggaacgtcgg aagaataaat ttcaggagtc ccaaaaatat acagcagagt
     7381 atcagcaagc gtaccaggta gcaattgtca taaaagacgt tgctttgcgg aataaacaac
     7441 tggcttcatt agccaaagtg attctattag gaacagatga gtatgggctc ccttatgtga
     7501 tgaactttag tgaattgaac caacatgtga ttattccagc gactacaggt tcaggaaaaa
     7561 cgacggtgat ccaattacta gcagaacatg cgtgtaagtt tcgtatcccc gttatcttag
     7621 tcgatgggaa aggagcagga gagactttac aagcgttaaa agaagtggca gaggcataca
     7681 atcgcccgat tgaagtcttt gtggacggtg gcgatttgcg ttataatcct gtaaaaaatg
     7741 gcaatgatat tgccattcga gataaattaa ccaatctagc agaaacagaa agcgtgtatt
     7801 attcgacagc agccaaagcg ttattacagg taacgattca gcttttagac gagttccaaa
     7861 agagccataa aattgaaaga aatctagttt ggttacaaca ttatttattg ccacgaaacg
     7921 tcttacattt gtttgcggat cgtattcagt tgaaaaatcc agatttattt aaagaaaaag
     7981 tggtcgtaga aaagaagaaa accacgaaga aaaaaacgaa aaagaatgaa gcgatggaca
     8041 aaacagaacg tgattttgcc gatgtggaac agttaagtga agagcgaatc gttgaacatg
     8101 atcgtaaaac cgttgactta gaaacgtatt atctattatt aaaagaaaac atttctctca
     8161 tgacagaaat cgagcaaacg ttgtttaaac ggttgtttgt tcgttacgaa cataagaaga
     8221 acccgtttta tttgtatgcg acttctgaga atttacagac caatatcaat atgttattgg
     8281 atagtgagct tggtgaactc tttgatactt ctggaggaaa aagagaatta gatattcaag
     8341 aagttgttaa agaaggaaag attgtttata tctcgttaaa cggattgatt tatagtgagt
     8401 ttattcggac attggctcaa atgcttgtgg gagatattaa tttctttgcg tcagaagtgt
     8461 ataaacaagc aggagaaaca gccaatcgct cgaaagtcct agtaatattt gacgaaccag
     8521 cttcttatct aaatgataac cttatagact tagtcaataa gggacgaggc gctggggtgc
     8581 atgcggcgta tgcccctcaa acgcttgcgg acattgatag aattgatcct gtccttcgaa
     8641 aacagttgat tgggaatgtg aatacgttct ttattgggaa gacaaatgac ccgacagaaa
     8701 ttgattattg ggcagattta atggggacag aagaagacat tgaattaacg gaaatgacga
     8761 cacaagaagc aggttattct gatatgggaa aggttgattg gacaggagaa agaggcacga
     8821 aacgagacgt agaccgcttt aaattcaatc cgaatcgcat tcgagcattg agaacaggcg
     8881 aatttgtggt gtatcgaaca gctgaagacg tccaggaacc accgcgagtc gtctatattc
     8941 ggaaaccaaa tttggagaaa caatatgcga aataagaaag ttattgttag gatacttatt
     9001 gttagtttcc tcttatttgg ttgttttaaa ctgataaaaa ctaacgaaga atcaaatgga
     9061 aaactaatca atagtaattt agaagaagta ctagaacttc aaaaaacgaa tcaatcattt
     9121 tttgtgtatc tagcaagaaa tgattgtaaa aaatgccaag aagttgaatc tttcttggaa
     9181 agatacgaca aaaaattctc taggaatgta tatcgaattg aaacaagaaa agaaccaaga
     9241 caagaagaac ttcaaacttt tttacaacgt taccatgtta aaagtgtccc aagttttctc
     9301 gtgattcatg atagaaacgt caaaaaaatt gagagaaaaa cattcttttt tgaaaagaca
     9361 ttgaatcaat ttcttcctaa tttttagtga aaattgaaag agaatacgtt gaaaaaaaca
     9421 aaaaattgtt atgagttcaa atttttttcg atcgtatttg tttttctttt gaaacaaatt
     9481 cactcatttt ttgagcaaaa atgtttcaaa atttgaacaa aaaccaattg aaaattgaag
     9541 caaatttcac tcgaattgaa tcgattttga gctaaaatca atagaaaaaa taaacgtatt
     9601 ttgtttgaaa tccgtttatt ttttttacgt ttcagtatta gtaaggatta tttgagggtt
     9661 atttagagat tatttaaagg ttatatgtaa taagtggggg gagggctgtc ggcgcttggt
     9721 atgaaaggca ttctccgact tccaggtttt tgaccatttt ctctcaacgt ggaaaaaccg
     9781 ccacttatga gagaaaatgg tcaaaaacct aagcgggaag tctccgaata acataccaag
     9841 cgccgacagc caacagataa agaatacact actaaaaata gtagtagcat aaaatttaaa
     9901 agggaaaaaa tcaaccccta ccccagtctt ggggtctcaa aactaactcc cccctaaccc
     9961 cctctttcag cctcttttga aagaggggga gaatcaaccc cacggccaac gccccagtgt
    10021 tcttggggct gtccagaaca gaaacttgcg agagagtggg ggtaaaacaa gtgtagagga
    10081 aacttgaaga cttgttcgaa cagaaacttg cgagagagtt ggagtaaaac aagtgtagag
    10141 gaaacttgaa gacttgttcg aacacaaact tacaagagag gggagtaaag cagttgcaga
    10201 ggaatcagga agatttgttc gaacagaaac ttgcaagaaa gcattattaa attgaaggtg
    10261 atgaaatatg aagtatttat tgcatggcta ttttattgat tattctactc gcaatagtag
    10321 tactttagcg attctatgtt tgcttcatga attgagagtg gttactagaa aacagctata
    10381 caaactattg aattttgaca gatcaattag taaacaagga gtaacgaatg ctttagtgag
    10441 attatatgaa aaacagttaa tcgatcgaaa aaaatatgga acagaaatgt attattattt
    10501 gacgaaagag ggacatcaaa gcataggtgg gtattatacg ttgccgaaag ttcctgaata
    10561 taatttgaat caccatttac aaattaacaa ttatttgatt aaaggaatgg cattggcaaa
    10621 agaccaccca catttaaaac ggattatttc agaaagacgt accgtctatg aaacgaagga
    10681 tttaaatcct aagacaaaag gaagaaaatt ttttgtgcca gattttattt ttcgttttcg
    10741 agacaaagaa aatcatgaga tcaattggtc ctttgaaatt gagctaacgt tgaaaacacg
    10801 tcgtcgctac cgtgaagcga tttttccgaa atatattcgt gagttgcgaa cacgtcctga
    10861 atcacattta atttatgtga cacctagtgg cgcaattcaa gaagaacttc atgcgtttaa
    10921 gcgtattttt ttcaagcgcg aaactgaaaa agatgcgagt caacaagaat tgtttgagcg
    10981 gttgcatatt ttttcagctt tagagtttga agagggatta aaagcattat tagagaatga
    11041 tccagcagtc aattggcgga cagtagaacg agaaaaaatt tatccgaatg gcgctgttca
    11101 aagtcgtttg aatttatttg aagaatcaac tgaaaatgag gaaaaaccaa aagtgaagtt
    11161 tacgcctgaa gtattgtaaa agagggtgtt cgcttcgctc tttttttgtg ccagtttgct
    11221 ggcaccgttc agacgcaagc gtctgaaccg gctcgttttt aggaaggact aacttgtttg
    11281 atttatccgt tgtcaaagaa gtggacgttc gagagtccct aatcgtatag cagtcattcg
    11341 gcgagtataa cctaaaacat aacctgccaa ttgccaaggg taaaatgcac gctgcgcgcc
    11401 cttggcaatc gtcagaacat gttttcgagc cggttatgcc gaacgaccgc tatacgaaaa
    11461 gggcctctaa acagaagtcc actttgatcc ttgacaactg aaacccaaaa tcaaacaagc
    11521 aggccttcgt ctaaaaacga gccgaatgaa gaccttgggg gttgcacccc caaacccccg
    11581 tgcccccgac acactaccga aaaggtgggg gtgggtggcg aaaaaatcaa ataaaacatt
    11641 ctattcaaga aaggaagaaa caaccatgac aactaaaaac gagcaactga aagaacaggc
    11701 aagaaaagag ttgcaagaaa gaggtttgat tattgagggg agctttgaag gagattttga
    11761 aacctatatt ggttgttatg caagaccgat aaacaaacct acagccttag acccaatgaa
    11821 cgaacaagaa gtaaaagaac aagaaaaaca tgcagtgaat ggttttccgc aagatttcac
    11881 tgaatggtat gagtgggaaa ttaacaacgg gaagcttgaa aacttcttat aaaagtcaga
    11941 tgacgtgcaa agagaatgat tctctttgta cggtaaaaat ataaagaaag aaggaaaaag
    12001 aaaatggcaa aaaacacacc ttatcaagta ggagacattt tttatagtag ttggggctac
    12061 gaacaaacga acgtgacttt ttggcaaatt gtgaagttaa cagaaaaaac agcatggttt
    12121 cagcctattc aaaagaaaac ggtcgagcat tattcgtcca tgagtggaaa agttgttcca
    12181 atgaaagaca agtttataga gtatccttta gcaaaaacta aagacagtat tgtaaaaaaa
    12241 cgaattgatc cagaaaccta tcctgatatg ttatgtggtt atcgtagttg ggacacgttt
    12301 catagatata aaaatcaagc tgtttatgaa agtagttatt attaagggga tagtttcccc
    12361 tttcaaaaat aataaataga agaatggaga gaaaaaatca tgatgaattc agtgaattta
    12421 gtaggacggt taacaaaaga agtggattta aaatacacac caaaaggcaa tgcgacaggc
    12481 acatttattt tagctgttaa tcgaaattat acgaatgcaa acggcgagcg tgaagcagat
    12541 tatatccgct gtgtcatttg gcggaaagct gcggaaactt tagcaaaata tactggaaaa
    12601 ggaagtttga ttggcattaa tggacgtctt caaacgagga gctatcagaa tcagcaagga
    12661 cagaccatgt atgttacgga agttctagtg acagatttct atttgttaga gagtaaagaa
    12721 atcaatgagc aacggtcaaa atcaatggcg aataatcaaa ataattcttc aattccagga
    12781 aatgagattc aaatttcaga tagttatttg cccttttaag tgaaaaataa ttttgttcta
    12841 tacctgattt cttcaggtat agaacaaaaa ataaatagag atgaggcgat tttttatgaa
    12901 aatcattgac agttacgatc aacgaaccat gcaatatttg ggctcgtttg attatacaga
    12961 tgaaaatatt attaattatg ttgcaagttt acctccttat caaagcgttc gcttggtaga
    13021 ttttgcaagt gatgaattgg tattaacaac gatgggcaac tttttagatt atgtcggaga
    13081 caaaaaatgg ttggaaaaaa ttttgtctaa attgattgcg aaacaaacag gagaagaacc
    13141 cataaatgcg attcaattgt tcaatcgata tgaacaagaa aacaatgatt aaagaggaga
    13201 gaaaaaatga aaagattaaa acgaagtatt tttattgtgc tagcattgat tgattttgtt
    13261 gggacaggtt gtgaagcaat tgacgttcaa aaagctccct tatcggaaat aaaacaagca
    13321 ataacaaaac aagatgaaga acaaccgcta aatacacaag tagaaatagt gaatcaaaat
    13381 aaaccgagtt tttctgaaga agatttttcg ttagaaaatg gaaccaggca atttttttca
    13441 gatttggatc gtttaaatcg ggtaggtgta gcaagtgcac ttttgcataa aagcaggatg
    13501 ccaacagaag aacgagaccg attatacatt aagccaactg gctggaaaca aaagaaaatg
    13561 aagaatggcg attatcttta taatcgttgt catttgattg gctatcaacg tgcgacaagt
    13621 ttccccataa gtatgtaata cgaaacggga ggattaatct taagattaat cacaactaac
    13681 ctatttgaca gttggaatga aagctatcat gtctgctgaa tcaactggtc tgatactcct
    13741 acatgcaata aagtctacta ggaagttatt gtatgaagct aggtaaaggc ggcagaacaa
    13801 tttctaagtg tggaatcata tgaagttgga tatgccggga cgctataaat tactcagggt
    13861 gagactgttt ttaattaaac tgacgaactt gcgaatgtac gggtctataa ccgacttata
    13921 ttcgaaagat ataagattgc gaatgcaaag aacattatcg aaaagtaaga atgttggtca
    13981 tgaaattcga gatggaagac tagcggtgta tctgtcaaga gttggtaaaa catgtttgat
    14041 tgattcagat gagagtggga atgcgacaaa acgttttaca gaagaaatag agcaactagc
    14101 agaactgaat atacgatatt tttaaatact ttagcggaga ataaaaaaat cctctacaga
    14161 aaactacgga gggcaacatg tgctattgtt tcaacaatac atttttaatt tttaccataa
    14221 caaaaaagta cttgaaaaaa aacagaaata tgttaaatta aaaatgaaaa aatggaagcg
    14281 gttacttatt cttttgaatc taagataaaa attcaaaaga ataacataac ccaaatatga
    14341 ttatacttga gattctaaat atttatcgaa aaggaatttg ttttaaggaa aaatgattct
    14401 atggacggtt attgatttat atttaccaaa tggctaataa aatagtattt cttatattgt
    14461 atagtttttg tttttatgtg aaaacatgag caatagaatt gtactagtag gataaggatg
    14521 gatggaaaat gaaaaaaact atttttgttt tagcgatttt tatatgtttt gggctacttt
    14581 tggtaggcta cttgttgttt aattctaatg aaatgagaac tatttctacg aatgacttgt
    14641 taaaaaaaag tgaattaaag gataaaaaaa aagaatctct tgcctttata atgtttactc
    14701 aagaagagtg tgagtattgt atagcaataa atccaatcat agatagtgta aataaggaaa
    14761 ctttgaataa gataaagaaa tatgatatat ctgtagattt tcaatctaaa caaaagtgta
    14821 aaattaagtt tgttccgact attgttgcat atagtaagaa taaagaagtg gggagaattg
    14881 acaatgaatt agataatttg tatcgaaaaa agcaagatgg ttcaattgta atagacaaga
    14941 aaaaaacaaa gacattgctt atagattgga tgaaaaaaat ggataaacaa ggagaatatt
    15001 tgtatggaga agattaaaca gacatttccg ttaattaata aatattggaa taagaaaaaa
    15061 atttttatat tttttattac gtttctgctg tcatctttat tttctctaat tttcccgtat
    15121 ctatttagta atgttattga tttatattta gagaatgagt tatcacagaa tattgtgcga
    15181 atagtaacga ttcttatatt tcagttattt ttagtcttaa ttaaagaagt gtatggatat
    15241 ataaaaacta tttatgcatt tgaagttgat ttatcaatga aaattgattt tcttaaggtt
    15301 ttacaaaaaa aaaatctttt tttttcgcaa acgaagcatt caggagaaat tcaatttagg
    15361 atgttttcgg atatttctac tatcgtaaat actatcttta ttttttctgt tgaattacct
    15421 gtgataggcg ttttagttct tgtagcggta tttattataa gtaaagtgaa tcttattttt
    15481 ctgatttttt cactaataat gatgggagct atggtgttac agtataattt atttcaatta
    15541 ccgcttaaga aaacaatcga gaatacaaaa agtgttaatc aaagtattac aggagaattg
    15601 aatgcacatt ttgatcaatt agaaattata agcgcatata attttgagaa tatctctatt
    15661 ttaaaattcg attctagatt taaagaatta tttaatgttt tcaaaaaaca aaataaatta
    15721 ctaacactgg gaagctgttt ggcacaggtt attttccaat tatggattgg aggaatattt
    15781 cttattgatg ttattttatt agctaaagga acaataagta ttactaattt tgtaatagta
    15841 tttatgtacg ctaacatggt ccctacttta tggactactt taattagtat tatttggaaa
    15901 tggcaagact attcagtgtc attaaataga tattacgaat attattcaaa taacaacaaa
    15961 gtggctggca caaaaaaaat aaataaaata aattctatta ctggtcaatc attaaatttt
    16021 tcatataaca aaagaaaggt attaagcaat ataagttttt caactgaaat gaataaacta
    16081 ttcatgatat caggagaaaa tggaacaggg aaaacaacac tgatgaaatt atttactaaa
    16141 cagttgccgg ttagtaataa tcaattattc gtcaacggga tagatattaa tatgattgat
    16201 aacgaatcat taagaaggtt aatttcaatc gcaacacaaa taccatatat aattaatagc
    16261 tccgtgaagg agaatatttt aatggggact aaaaactatg ataaaaaaaa attcagtaaa
    16321 ttaatagaac tattgggaat aagtaaattt atgacgaaat taggtattac tttggacgac
    16381 aatatttatg atgaaaaaat attattatct ggaggagaaa aacaaaaaat aagtttagct
    16441 agggcgctta ttaaatcagc ttcagttatt atattagatg aaccatttag aaatttagat
    16501 aatgaaactc gcgataattt gagggattat atactaaata caaaaaaaga agtttgttat
    16561 ataattataa ctcatgatca cattctagat aattttttgg attcaaatga tgaaatatta
    16621 tatttaaata aggagtatga taataatgaa tagcaaaaaa cttcagatgg gtgaaattgt
    16681 tagaggtcct agtcaaatat ctattgatgt tacatataaa tgtaatttta gatgtaaaca
    16741 ttgttacaat gcgagtggtg gagacaattg ttttatggat aatgagttga ctgatgatga
    16801 actgttagaa cttacaaaag atatatgtaa tttaaagcca tataatattt gtttctgtgg
    16861 aggagaaact ttactgagaa aaaaaatagt tttatcttgt gccactttat taaaagaggc
    16921 aggtattcct aatattgcta ttgttactaa tggatcatta gtaactcagg atgttgcaaa
    16981 agaattattc aaagcaggta ttaatcgatt tcaagtaagt ttagatggaa aaaatgctag
    17041 tagccatgaa gcacttagat tatatccaaa cagttttgaa aaggcaatta atgctatcaa
    17101 attatttaga gaattgtccg aagaagtgga tgttgctttt tgtcctacta gttttaatta
    17161 caatcaatta cgagaagtat atcaactagt aaatactctt aaagtgaagt ctttaagaat
    17221 tcaaccattt atgcttagtg gtaggggaat gcaaaatagg gaaatagtgc caactgtttc
    17281 tcaatataga aaactagttt ctgatattaa ctatattaaa agaaatgaag attataccaa
    17341 gatagattgg ggagatccaa ttgatcatat catccgacac agaactttat tgaaagattt
    17401 ttcgaatttt gtttctatta ggtcagacgg aggattaatg gcatcacctt acttgccaat
    17461 tcaagttgga aatattaaat gtcacagtct ttctaaatat tggcgagcag gactttataa
    17521 agttttttct ttagatttag ttcaagatat agctactcaa gtttcttcaa tacaagatat
    17581 gagtaaagaa ttaaaaaaag gacatattaa atggcttaat gaagatatag aaattgatat
    17641 tctcgatgaa tacaaacatg atattttaat ttaggtttat cattggaaag tttagataaa
    17701 caaagtactt ttagaaagga gggaaaaaaa tgatttctga acctcaaatg aataatatgt
    17761 atgaaagccc aactataatg ggagctggtg aagctgcgcc aagagcttgt tcagcaatta
    17821 tggtgtgtgt gttattagca tctgcaggag taggtatcca agcaggagcg gtgtatactt
    17881 atgctgtagt tgctacagta gctgtagctg cagccgcagt ttggaaaaca gtaggattag
    17941 ttggttagga attaatagga gactaactgt ctcctattga ttccaatgat taagctaagg
    18001 aggaatttta agaaaataat gaaatacaat aatttaaaac cgaaattact taatgagaac
    18061 atagaatata taagagaaga aaattatgga tatttaacca tagttcccaa aaatggaact
    18121 gaattgaaag ataacgtttt caatccaaca gtaagatata tattggctaa ctgtactgga
    18181 aaaaacagtg taaaaaaaat agcgaatggt ttaaaaaaga agtttcctag tttagaatat
    18241 gaaaaattct atcaagatgt tgtaggaact attaaatttt tcaaacacta taatattatt
    18301 gatataggag attatagtat ggaagaaaag aaaatatata aaattgaaca aaatttagaa
    18361 ttagaattac taaatgaatc acattatgaa gaaatttata attttatcac agatcatcct
    18421 gaaagtatgt attttcatga agatatttat catcaaggaa atagtaaaga attagaagtt
    18481 gtccttagat ataatttatt ttcgtttacg agtgaatttt actttttaag acataaagag
    18541 agaattgtag gaatggtttc tataattgtt aatccatttc aaaacttatc ctacagtata
    18601 gtaaattacc tcatatgtga gcctgcatat actacgacta ttttgacaac agtattagaa
    18661 aatattaacg aagtttcaat tttagaagta aagaagctag tgattcattt gctaaattca
    18721 acagaagatg aatcactaat cccctattta aaagattcac atttttttaa agaagcggta
    18781 ctatctaaag aatatagaga gcatgatgtt ttgagatacg cttacattta ttaaataaaa
    18841 tttctgatag tattttaata attcggatca tatcataaga tataggaggg aaaagaagtg
    18901 attttattga ttttgtctct cgtattacta gttgtatcta atctaatgaa aaataatcct
    18961 cctacaaaaa ttaattcttt ttatggcttt agaactaaaa agtcaatgaa aaatgaaaaa
    19021 agttggcatt tagcacaaaa attgttgggg gatttttcaa aaaaaaaagt tatcccagta
    19081 tttttgatgg gtattgtttt tttcagtata gagatttata gtttagctat tttaaaaaat
    19141 gaaaattttt tcgttagtgt attaatgggt gaaagtttaa tcctaatact gattttgtgt
    19201 ttaattgttc atgaagtgaa taaacaattg tgaattaaaa aaataaaaac ctagtataat
    19261 cactttaata aattgttggt agaaggtgat caaaatggga gtaaacgaaa tgatgaaagt
    19321 cagattgagt ttttaaatga taccttcctc agaacttcaa aacgaattca aagattcgtt
    19381 gacaaatctt gagcaagatc atttaaaaaa atattttctc ttcatttgac gaaaaatgtt
    19441 tagaaatctt atataagtga agataaagta attggtgtaa ttaattgtag agaatagcct
    19501 ttgagcaatt gaagcgatac aattatttag aaaatgaact tcctccagca tactacatga
    19561 ttccttacgg gacgatgtta aagaagaaag caagaaattt tagaagtatt agtagataaa
    19621 ctaatgggct tttctcaatt taagggagta gcccaaatgt ttatcaaata tggtgcagat
    19681 acagtagcaa ccatgtttac agaagaatat caagtagact attatgaagt aatagataaa
    19741 tgtgtacatt ttgtttatga aaaagtgtgg aattttatga attttgcgat aagtgaagaa
    19801 atcttacgag tacaagaaaa cagaacagga atatttacca gagacagaaa gcttaatttg
    19861 attgcgcatt atatacggaa tcgctggtca tcaataccaa attttaatta taaaaaataa
    19921 ttatgaacaa tcttttatac aatatacaca ataaatcaag tagtatttat gaaaaaagac
    19981 ttcaaaaaca gaatgatttt ctatcagtct tagaaattat agagctaaaa attttggtca
    20041 cgcgtagaaa cgcgtttttt ttgttttttt gatagtaaag aacgtacaaa acaatgagaa
    20101 tttaaaagat tgtactacca gtttttttac cttttcattt gagtttagag taagtaaaat
    20161 attactataa aaacacatat tcttgaagaa atcttttttt tgctgtattc atttagatta
    20221 tgatagagaa aatacgtgag agggggacct gggatgtggc tatctgatga aaaaattcag
    20281 tggcgtagct atcagaagcg tatattgact aactttgcta cttatttaaa taacaattca
    20341 gtccatctag tggctccacc aggggctggc aagacctttt taggaattga gatgatccga
    20401 cgtttggatt gtaaaacctt aattttggtt ccttccctaa tattgaagaa acagtggtta
    20461 acaacaatcg aggaatattt cttagtaaat ccagcagata gttgctatct ttctggggcg
    20521 attgaacatc ctagtcatat tacagtggaa acgtatcaga ccatttacaa ccgactgaag
    20581 gaggagccaa cttatggtga tcagctggag ctgttagttt tttgatgaag ctcatcattt
    20641 aaaaaaaagc tggggtgaat tgttgattga cttgaagaaa aaacaaccga atctgttgac
    20701 gatttccttg acagccacgc ctccgattga tagctctcag acggagtgga agcaatatat
    20761 tgagttaaaa tggcctgatt gacgaagaaa ttagcattcc cgagttggta aaggaggagg
    20821 ttctatgtcc tcatcaggat tttctatatt tgattccggc taatcaggaa actactcagc
    20881 attattttac ctttttagag aaacaaaatg agctggtgga agcgttaatg gaaagccaag
    20941 agacggtgga ttatttgctt tctttagaat ttgttcagca tccgcttgag cattcgaagg
    21001 aaatttatga aaattttgag ctgttcacta gtgcattaat ttacttgaat catcataact
    21061 atgaattgat acccgaacat tggcagctat taggcagttc taagcagcaa ggtaaaaaag
    21121 cttcagttcc atctttaggt aaaaaagagc tgatcactat ttatcagcag atgtttgaag
    21181 atcaaccgga attgcctatt ttttatcaat tgaaaaaagc tggatgattg tttgatgagt
    21241 cgctgcattt gtttccagag tatccagaca ctgttggtag tcaggcgatt cctgagaaaa
    21301 agcaagcagt ttgtcaaatt ttgttaaaag aagaagttca gctgaagcaa gaactgacag
    21361 cggtcatttt gatggatcgt atttgcaagg aggtattagc aggcagtgat ggtaccttag
    21421 cttatggcgt ggttcctttg tttgatgaag ttgttcgtct attaggtaac actcagcaaa
    21481 tcggtgccat ctgtggagag ttcttactgt taccgaaaat gctagtaact gttgagctgg
    21541 cagaactagc gacgggtatg aatgaacatc cttatatgtc gggttatttt tatttgacgg
    21601 tggatgacaa aaaccgccaa gcattgatgc aagcagttac tcgcctatta accgaaaaga
    21661 agttgaatat tttggtagga accgttagtt tgctaggaga aggctgggat tgtcctgcaa
    21721 ttaatctgtt gatcttggct aatcaagctt ccacttttgt gcaaacacaa caactacgag
    21781 ggcggggctt acgcctaggt gcacaaaata aagtaacgaa catttggcat ttagcgattg
    21841 gattaccggg agttcctctg tctgaacagc ctgatttgaa aagaatgcta cggcgtttgg
    21901 gcaatatttc aggtctgacc tttgaatctt caccaaaaat tgagagtggt ggcgatcgct
    21961 ttgctttacc actggaatat acgaccacca cgatggaaac ttatactcag tcgatgttct
    22021 atttttcttg tgaacgaagt cggttgaaag aacaatggag tactgcttta gctgatggct
    22081 ggcacctatc tatgccaatc gtcgttagga aagtcgatca ggtaaagcaa aaacaagaaa
    22141 cttcattgcc gatagtagta aaaaatatag agcctctaag cttctggtca gctatattta
    22201 aaggtaactt gctggaatat ttccaaattc gtaggcgaca aaagctatgg gaaaaggaat
    22261 gtgcccgcaa aaaaagcttg gccatatgcg ttcaaacgac gttgcagcgc cttggtgaag
    22321 ttagtgatag aaaagtaatc gtagcggctg atcagcagga atttagatgc tatcttgaga
    22381 atgctggcta tcgagaagga cagcttttta ctgaagctat gagggagctt ttagcaccgc
    22441 tggagaatcc gcggtattta atcgaaggga aaaagatagt tctgacggta ccagaacgct
    22501 ttggccgaaa taagcaaatc gctaaccttt ttctaaacga attaaatcaa tcgtcgctgc
    22561 ccaaatgtca gttggtgtat actagaaatc ctgaaggacg ccaaaagcta gtgacttatc
    22621 gaatgaggca agtcttccag acccgcgatg aagtaattat tcaaaaaaac atttggacgt
    22681 aagttgatga aaataaattt tgctagagta gaacaagtcc gagaattaag ttgctactta
    22741 actgataatg ccaaggacag gagaatcttt tcttgacgac tgaaagaaac tatttgactt
    22801 atataacggt gtgtgatgaa tatttgcaag gcattctctt aaaaagtact cgtaatagtt
    22861 tttttgaaaa caaaacagta ctttattaat aggactgaca gagcgatata agaacatctg
    22921 cagacagagg tgaatgcttt tctcatgatc gatgaaacaa tggtacttcg gatattcttg
    22981 cttttagttt gaaccgttgt gattttcttt gattcctaaa agtagtttta tagaatgaaa
    23041 atcaagcata cggaatgctc ctttgattgt gttgtggtga ttcaatctaa tggagcttct
    23101 atatgttttt ttatttgcaa aaagaaccga tagaatattt ttatcagtct tagaaattat
    23161 agagccaaga aatttcaagc acacataaaa acgcgtgttt tatgcatttt atgagcaaaa
    23221 ataactaaag accattacgt aaaacatagg taaatcagca ttcaatctca aattatgtga
    23281 tcaaatgttg atattatttg attataagtt ttcttttgct attatcagtc tgcaaataaa
    23341 cagattatag agtatgaatg tagatagttc aatctttgtt tacgacctca catgtttttt
    23401 tactcaagag aaagtaatat aaagtgacta tatttgacag aactttacat aaaaaaagta
    23461 gttagttttc tgtagtaatc agaaatgtaa ataaattaaa aaaaagaaat ttagcagtta
    23521 tatattttta aaatattttg ggtgagtagt caatacaaca ttttgttcat caagcattaa
    23581 atgaagatgc aaggatgtta agaagttagc ttcttcaggg tcttgcatgg tcaaggcttt
    23641 aaatagttga tgatgttgtt ccataatttt ttcccatttt aatccttccg tttttactcg
    23701 taaccaacga aatcgttcca agtgcgtatt attatcttcc aaccattgcc atacttcttc
    23761 ccgttctgca atttcaaaac aagctttatg aaataaatta tcattatgaa aaaaactacg
    23821 agcattcttt tgactaacag ctttttcttg ttctgcaatt atagtttcta aaacttgttt
    23881 cttttgatta ttcatcttgg cacaacactc tagcataatt tgtcgttcta aatgttctct
    23941 gacaaaacga gcattttttg ctaaatctaa gtcgattaat gagatataat ttccactttg
    24001 aggaattgta tcaacaagtc cttgatgacg gagttgaatc aatgcttcac gtattggagt
    24061 acgaccgata gaaagcgttt cttccaattt tttttctgat actttttttc caggttctaa
    24121 gtcagcgtag ataatttgct gacgaataga ttgataagct tgattttgga gactagtttt
    24181 aattgtcata tttaatccct ctcaaaattt catttttgaa tttgatattt tagttctatg
    24241 ctcgttatat aatatcaaat ttcaaagtaa catactagct attaaaaaaa caaaaaatac
    24301 tcaaataaga tcaaagagaa attaagcttt aatttaagtt agttctaaaa aatatatatt
    24361 ggtgtgcttt tactaaaaaa agacacaata attctgacat ggtagaaaaa agaatatttt
    24421 tagttttttc ttattgacag cgattacatt cgattttata ctacaattgt aacggaaact
    24481 aacatatcag tatggagggg taaatatggt tttgattaaa gactggcaag attctatcga
    24541 aggattgaac aaaatgcaag ttcaactacc gtattttaat gtcgaagcgg tcaaacaaga
    24601 aggtcagaaa catccagtgt ggcttcattt tggtggagga aatctttatc gaggatttca
    24661 tgcagaaatt gctcaatgct tagcagatca aaatgaatta gaatccggag tagttgtgtg
    24721 tgagacattt gatgaagaag tagtagaaaa agcttatcat gactacaaca atgatatttt
    24781 ggagattgtt atgggtgaag atggtgaact caaaaaaaga cttttagcag taacagctga
    24841 tagtttatat tgcaatccaa agaatataac ggactatcaa agagtggtta aatattttga
    24901 agatcctaca ttacagttta caacgtttac tattactgaa aaaggataca gtttaaaaga
    24961 ttctaaagga caattttttt cggtagtgga agcagatata aaatctggac ctaccaatcc
    25021 tcaacatacg atgagtattg tgacatcctt gttgtatagc aggtttaatg ctggagagtt
    25081 accgattgcg atggtttcta ctgataattt ctcacagaat ggtcaacgtt ttcaagatag
    25141 catttttatg attgcagaag cttggcaaaa aaaaggatac gtttctgaaa aatttattgc
    25201 ttatttaaag gataaagaaa aagtgacttt tccttggagt atgattgata gaattacgcc
    25261 aaatccttcg gaaagtgtca aaatggattt attgaaggcg ggtttcgaag acttagatat
    25321 aattcatact gcgaaaaata ccaatatcgc tgcttttacc aatactgaaa aagtccatta
    25381 tctagtcatt gaagatagtt ttccaaatgg tcgacctaaa ctggaagcgg ctggtgtgat
    25441 tatgacagat agagaaacag ttgataaagc agatatcatg aaggttacga catgtttaaa
    25501 tccgctccat acggcattag caataacagg ttgtttgttg gggtataaat caattcaggc
    25561 agaaatgcaa gatgatgatt tggttggctt aattaaaggg ataggttatg tagaaggctt
    25621 accagtagtt gaaagtccag gcgttattga tccaaaacaa tttattgatg aagtaatcaa
    25681 taaacgtttg cctaatccct atattccaga tacaccgcaa cgaattgcaa cagatacttc
    25741 tcaaaaagta gcgattcgct atggtgaaac aattaaaaag tatgtcgaaa aagacggaaa
    25801 tgcagaaaat ctaacattta ttcctatcgc tattgctgct tggttacgtt atttattggc
    25861 agtcgatgat caaggaaaaa attttgtgcc aagtcctgat cctttattgg tagagttgca
    25921 ggaaaaattc tcagtattga aattaggtga acaagatcct gcaaagattc attgtgcggt
    25981 acaggaaatc ttagaaaata aggaaatatt tggtttagat ttatatcaag ttgagatagg
    26041 aaagaaaatt gaaaaaatct tttgcgaaat gctagtaggt cctcaagcag ttcgtaaaac
    26101 aatcaataaa tatataacag aagttggagg aaagtaacta tggaaatgac ttttagatgg
    26161 tatggggcaa atgaagaaaa aatctcacta caagaaattc gtcagatccc agcaatgaaa
    26221 ggaattgttg gaacattaat ggatatacca gccggagagg tctggccgag agaacgaatt
    26281 aaagctttaa aagatgaagt cgaagccaat ggccttacct tgaaagttat tgagagtgtt
    26341 aatgttcatg atgatatcaa aattggtttg ccaacacgag atcaatacat cgagaattat
    26401 aaacagacga ttcgcaattt ggcagaattt gggattgagg ttatctgtta taattttatg
    26461 ccagtctttg attgggtaaa atcagatttg gattatcatt tagatgatgg gtcttctacg
    26521 ttagcctata tttcaaaaaa tattcctact gatccagagg aaattattag aaaagtagaa
    26581 aattctacta ataattttga attgccgggt tgggaacctc atcggttatc acaaatcaag
    26641 caattattag aagcctataa agaagttgat gaagcgaaat tgcgagagaa ctttgcatat
    26701 tttctcaaag caattatccc gacatgtgaa gaagttggga ttaaaatggc agttcatcca
    26761 gatgatccac catattctat tttcggatta cctcgtattg ttaaaaatcg tgacgattta
    26821 gaatggttgt gtaatgtagt ggattctcct tctaatggta ttacgttatg tactggaagt
    26881 attgcagaac gaccagataa tgatgtctat gaaattctat ctgaattttg tcaacgggat
    26941 cggattcctt ttgcacatgt tcgtaatatc aaatatttaa gtgataatgg actcgatttt
    27001 tatgaagcgc cgcataaatc agaatatggt tctctagata tgtataagat attaaaaaca
    27061 atgtacgata atggatttaa tggctatatt cgtccagatc atggtcggat gatttgggga
    27121 gaaaccggta gacctggtta tgggttatat gaccgagctc taggtgcagt ttatttaaat
    27181 ggtatttggg aagcgcttga aaagggggcg agtaaataat tatataatgg agtctattta
    27241 gaagaaaaat tttctcttct aaaaactaag ggaaggaaag gagtgcttaa atatgttttt
    27301 agataagaac tttttattag agaatgaaat agctaaaaga ctttttcata aatttgcaga
    27361 acaccaacca atcatcgatt atcattgtca tttagaccca gcatgtttgc tagaaaataa
    27421 gcaatacaag aatttgactc aggtatggtt aaatgataaa ggagctggag atcattataa
    27481 atggcgattg atgcgagcta atgggataga agaagagttg atcagtggtg caggtaatga
    27541 ttatgaaaaa tttttagctt ttgtaaaaac agtggagaag gcgccaggta atcctatttt
    27601 tgaatggtct catttggaat tacaacgtta ctttgatatt gatttgacaa tttctgagaa
    27661 gaatgcacca gaaatttggc gacgtgccaa cgaaaaaatc caaacggatt cctatcgccc
    27721 aaaagaattg ctgaagatga tggatgtgaa agccatttgt acaacggatg atcctacaga
    27781 tgacttgcga tatcatactg agctttctaa acaggataat tctttcaagg tattacctac
    27841 tttccgtcca gataaggtct ggagtatcaa atcatcagga ttttcgaact atattttaaa
    27901 acttagtcaa gcagcgggta taccaatcaa aaactttaat gggttgaagc aggcattgag
    27961 ccaacgagtg gattattttc atcaaattgg ctgtcgatta gcagatcacg gtttgaatac
    28021 ttatttttat gaaatagcag atgaaaaaat actcgacaat ctgttaataa aagctttagc
    28081 aggaaaaacg gatttccatt tatcagaaat tgcacaattt gttacaggta ttcaattaca
    28141 tttgatgtct gagtatacaa gatatggttg gacgatgcaa atgcatttaa atgctttaag
    28201 aaataattca agtatattaa aggaacaaat tggagtagat gtcggtggtg attcgatggg
    28261 ggatcaagct agtttgacac aaaatgttgt agcattgttc gacactgctg aaaagagtgg
    28321 gtgcttacca aaaacaattt tatattcatt aaatccaacc gattggcttc cactagcaac
    28381 agcaatgcaa agcttccaag ggggtatgat tcaaaaaatc caattgggat gtgcttggtg
    28441 gttcaatgat acagctgaag gaatgcgtca acagttgact acgatggcgc aacaaagttt
    28501 gttgtcgaat tttacaggta tgttgactga ttctcgaagt tttctaagct atcctcgcca
    28561 tgagtatttc cgtcgagtat tatgtcaatt actaagcgag tgggttaaag caggtcgaat
    28621 tccagatgat gaaatgtatc ttagtcagat agtggagaat atttgttatc gtaatgcgta
    28681 taattatttt aatttttttg aaatcgaaag tagaaaataa tacaaaaaat aaaagcttaa
    28741 atttatttat tgtaagttag tacaactagc tagagtatgt atccataaaa gaaggagata
    28801 gatatgaaaa aagtagaaat tttaagtaag ttagaagcgg ctggagtcat tgcggttgta
    28861 cgtggaaaaa ataaagatga ggccattaaa gcaataaatg caattactgc gggtggaatt
    28921 aaagggattg aactaacttt tacagttccg aatgctgaag aaataatcaa agaaattgct
    28981 aaaaaatatg ctgatcaaaa agatgtaata attggagccg gtacagtttt agatgcgttt
    29041 actgctcgta ttgcaattat ggctggagca gaatatatcg taagtccatc ttttgatcga
    29101 caaacagcag agatttgtaa tttatatcaa attccttatt taccaggttg tatgacaatt
    29161 actgagataa aggaggcact taccgctgga gtagaaatca ttaaactatt tccaggttct
    29221 gcttttggtc catctgttgt aaaaacgtat caagctccat taccatatgt aaatataatg
    29281 cctactggcg gagtaagtct agataactta gaagagtggt ttgaagcagg cgttgttgca
    29341 gttggtgtag gtgggaattt gttggctcct gttgaaaaag gtgattttga agaagtaact
    29401 cgaatagcta gtaaatatat agataagttt tatgagataa agaattatta ggagaaaaaa
    29461 taaataatac tataaaatgt aaaaaataaa tactaactct ttatacttca gagtatagta
    29521 cgtaagttgg cagttcaaca gaatgaaaac gcttaacaat aggaaggagc gcaaattata
    29581 tggaaagaaa atttggtcta agagacaaaa taggatatat gtttggagat tttgggaatg
    29641 atttcacttt catttttgct agttcttttt taatgatttt ttatacaaaa gtattgggga
    29701 taagcggagc taccgtagga acattatttt tagtagcaag atttattgat gcagttacag
    29761 acataacaat ggggcgtatt gtggatggat caaaaccatc taaaactggc aaatttaaac
    29821 cgtggatttt acggatgagt ggtccagtcg ctttagcaag ttttttaatg tatcaaacat
    29881 caatggcatc agcttctatg agtattaaag ttatatatat gtatgtaaca tatatactat
    29941 ggggaagtat tttttataca tctattaata ttccttacgg ttcaatggca tcagctatca
    30001 cttctgttgc agaagaaagg acagcgttat caacatttcg tagtatagga gctaccttag
    30061 caggtatgtt cattggcgtg ttaacaccaa tttttattta tacaagagat agttctggag
    30121 ctcaaattgt acgtggtgga tcaactttta caattgtggc aggagtattt tcagtgtgtg
    30181 cagttatttg ttacgtaata tgttataaat taactgtaga acgggttaaa agtgatgaat
    30241 cgcagttaaa agtttcattg ttgaaatctt taaaaggatt agctaaaaat agagcgttgc
    30301 tttctataat agctgcagct atttttttgt tgcttgctca gcttttaaca atgagtatga
    30361 acaattatgt atttccagat tattataata atgcacaggg aatagtttta atgaacttta
    30421 tcaatcctat tttgatgttg gtacttgttt cacctgtaag tttaattttc agtaaaaaat
    30481 acggtaaaaa agaattggcg tcagtaggga tgttcttctc tgctattgta tatttacttt
    30541 tatttttgat aaagcctagt aatatgtatg tattcttagt tcttacttct gttggatata
    30601 tggggttagg tatttttaat acagtcattt gggcgaatat tactgatgta atcgatgatc
    30661 aagaagtgaa atctggccaa agagaagatg gaactattta tgcagtatat tcttttgccc
    30721 gaaaaattgg acaagcttta gctggcggtg tcggaggttg gactctttct ataatagggt
    30781 atgattcgtt agcaaaagta caaagtggag aagttttaca aaagctgtat aactcttcaa
    30841 cgttgattcc agcagtttgt ttctttgttg taggattgat tttatttttc gtatatccat
    30901 tatctaaaga aaaagtatta gaaaattata atttgctaaa tcaaaaacat aactaggaga
    30961 aaaaacatga acagttggat attttgtatt gattcagatg gttgtgtcat ggatacaatg
    31021 acttataaac acaatctctt tttcgggcca attgctgcag agatttttga tgttcatcat
    31081 agggaaaaat tcctacagga ttggaataat attaatttat attcagagac tcgtggaata
    31141 aacagatttt taggtttagt taaaacatta gaaaaaaatg atgtaccatc gattaatcga
    31201 ttaaagcact gggtatctgt aacaaaagaa ttgtctaatc catctttgaa gagggaaata
    31261 gaatactctc agtcagagga tttattaaaa gctttaaaat ggtcggaaga agtcaatcga
    31321 cagattagtc aagctaaagg attagataaa ccctttttaa gtgctagaga tactataaaa
    31381 gctctgaaaa aatatgggaa gataattatt gtaagctcag ctaataagga agctattcaa
    31441 gaagaatggg aaagacatga attattatcc ttagttgatg aactgtgttg tcaagataaa
    31501 ggaaaaaaag aagacattat tcgaagtgta attgaaaatg gttgtgattt ggataagata
    31561 ttaatgattg gtgattctcc tggtgatcta gaagctgcaa ataagaataa agttttcttt
    31621 tatcctattt tggttaataa agaaaaagaa tcatgggaaa acttaagaac tgatatttta
    31681 tttaaatgtt tatcgggaga gtatatagat attgaacaaa aatatataga tactttttgg
    31741 aaaaatctta ctaataagta gaggagaaag aaaatgacta taaaagtaga cttacgaaaa
    31801 aaaccatatc atttagatga agatgctatt tcttggattg aagagacgat tgatgctatg
    31861 actttagaag aaaaaatagg tcaattattt gttaatatgg gatctagtcg tgataaaaat
    31921 tacttaagta gtatggtcaa tcagtaccat attggagctg tacgatataa tccaggaact
    31981 gctgatgagg tatatgatca aaactatatc ctacaaacag aaagcaaaat tcctttatta
    32041 attgctgcta atacagaagc aggtggtaac ggagcttgta cagatggtac cgaagttggt
    32101 gtagaagtaa aaattggtgc aacaaatgat gcgaaatatg cttatgaatt agggagagtt
    32161 tctggaattg aagcatcggc tattggatgt aactggagtt ttgctcctat tgtagatatt
    32221 aatagaaatt ggagaaatcc tatcatttct agtcgatctt ttggacaaag ccctgataaa
    32281 gttttagaac ttagtttagc ttatatgaaa ggtattcaag agagtggaat tttgcctgca
    32341 gctaaacatt tccctggtga cggtattgac gaacgagatc aacatctatc ttttagtatt
    32401 aatagtttaa gtacagaaga atgggataat tcttttggaa aagtatactc aggcttaata
    32461 gaagctggat taccttcaat tatggcaggt catatccata tgccagctta ttcaaaacat
    32521 tttaatcttg atctgaaaga tgaagattta cttcctgcaa ctttatcaaa agaattaatc
    32581 actgacttac ttagaggaaa actcgggttt aatggattag tagttactga tgctagtcat
    32641 atgttaggaa tgactggggc aatgaaacgg tcagaattat tgcccacttc tattgcggct
    32701 ggatgtgatc tattcctatt ttttaatgat ccagatgaag actttaatta tatgatggaa
    32761 ggttattaca aaggaattat ttcagaagag cgtttacgtg atgcattaag aagaatttta
    32821 ggcttaaagg ctaaactagg tttgcataaa aaagaaaaga ataaaatttt gttaccgaaa
    32881 caagaagcta tggaaaaaat tggtttaatt gaaaataaat caatttttaa agaggtagct
    32941 gatcaagcaa ttacactagt taaagataaa caagatattt ggcctattgt accagaaaaa
    33001 tataaaagga ttttactggt agatgtacat ggggtaaaag gtgggtttgg tgaagtagtt
    33061 gctggaaata gacctcaggc cattgattta ttgaaacaga ttttgtcagc aaaaggattt
    33121 gaagttgaaa aatacatttc acctgaagaa gaattgaaag gattatcgca agaagaaaaa
    33181 attaaaaata tagctggaat atatgctgct aagagaccaa tcaaagattt aactgaaaaa
    33241 tatgacttga ttattaatat agctaatgtt aacccaggta cagttcaaag aattgtttgg
    33301 ccagcaagta aaggtactcc ggatattcca ttttatattc atgaaattcc aacaattttt
    33361 gtatctgtac agtgtccgtt tcatttagct gatgtgcctc aagtaaaaac ttatatcaat
    33421 acttatgatg gaaaacaaga aactttagaa atattagtgg acaaactaat gggcctttct
    33481 gaatttaagg gaataagtcc aatagatgct tattgtgggt taaaggatac atttatttaa
    33541 tatttaaatt atataggagt gatttacatg ttatatccca ttatgacaga gacgaggcaa
    33601 gtaattgatt taagcggtct ttggagattt aagttagata caaatgatga cttacaagaa
    33661 gaacttgctt tagaaccaat ggttgaaaaa gagagttatc caatttcagt tccttcttct
    33721 tataatgatt tatttgaaac agagaacatt agaaatcatg ttggatgggt gtggtatgaa
    33781 aaagaagtga ttcttcctaa aaaattatta gaagaaagat tagttcttcg ttttggatct
    33841 gcaacacatg aagcaaaagt atttttgaat ggaaaattga ttaatcaaca caaaggaggg
    33901 tttacgccat ttgaatcaga aattaatcag tttataaaag atggaagtaa cagaattaca
    33961 gtggctgtaa acaatgttat tgacgatagc actttacctg tagggatttt gaaagaaacg
    34021 atacaggaag atggagacat aaaaaaagaa aatttggtta attttgactt tttcaattat
    34081 gcaggcatta atagacccgt taaattgtat acaactccga aagactatat aaaggatgtc
    34141 actattaata caaaaataat tgattcagat gcaaatgtag aatttaatat cgatacagaa
    34201 tggaccggag aaattctaat agcaatctat gacgaagaag ctcaacttat agcagaagct
    34261 aaggggaaag aaggagtgat tcaacttaag aacccaacac tttgggagcc tctaaatgct
    34321 tacttatata gtatgaaagt tagtttaatt aacgaaaata aagtgattga tacgtacaca
    34381 gaagaatttg gcattcgtag tattcaggtt tcgaatggga agtttttgat taataataaa
    34441 cctttttact ttaagggatt tgggaagcat gaggacacgt atattaatgg tcgtggtttg
    34501 aatgaagcag taaatatcaa agattttaat ttaatgaaat ggatcggtgc taattcattt
    34561 aggacttcac attatcctta ttcagaagaa attatgcgct tagcagatcg agaaggtatt
    34621 gtagttattg atgaagtacc tgcagtagga ttacatttga atttcatggc tactttatta
    34681 gatgacaacg tagaaaaaca tgatacttgg aaagaaatac ggacagaaaa tgctcacaaa
    34741 atggcgatta aggaattgat agaacgcgac aagaaccatc cgagtgtcgt aatgtggtct
    34801 attgcaaatg aaccagattc ggattctgaa ggcgctaaag aatattttga acctttagtt
    34861 gaattgacta aagaacttga ccctcagaag agacctgtaa caatcgttac ttacttaaag
    34921 tcaacaccag atatttgtaa agtaggagat attgtcgatg ttttatgttt aaatcgctat
    34981 tatggttggt atgtttcagg tggagaccta aaacatgcaa aatatctgtt gaaaaaagag
    35041 ctagatggat ggatggaaag atgtcccgat acgccaataa tgtttacaga atatggagca
    35101 gatacagtag ctggacttca tgatacagta ccaacaatgt ttacagaaga gtatcaagtg
    35161 ggttattacg aagcaaatca cgaagtaata gataaatgtg acaattttgt tggtgaacaa
    35221 gtgtggaatt ttgcagattt tgaaacaagt caagggattt taagagtgca aggaaataag
    35281 aaaggaatct ttactagaga tagaaagcct aaaatgattg ctcactatct aaagaaacga
    35341 tggtcatcaa taccaaattt cagttataaa aaataattta ccttttataa ggaatataaa
    35401 aaaggagtgg ctgcttaatt gccaactcct tttttttata aaaagatttt ctgtttaaag
    35461 aaggatattt ttttctagtt ttttcgaaaa aaaggcgctt tctgattgtt gtattagtgg
    35521 aaaggggaaa aaaggagcat atttatcatg caacaaaatt acaatgagaa atctatacaa
    35581 catcaatttg atgcctactg taaaaaagta ttacgaaacg aaactaagtc tattcgaaaa
    35641 cgaaacatta acataacaga taatgaggaa ccattgacga attcaaatga aggtaagtac
    35701 tcccaaactc attttgatga acaagatgtc tatttcttat ttggaatgga aatattaatt
    35761 tcagatcaaa agttatcggc agccattgat catttatcgg aaataagaag aaaggttatc
    35821 ctactttatt attttgcagg ttttaatgat actgaaatcg gtaaaatcct caatatgagt
    35881 acaagtggta tctggtatca acgaaagaag gcagttgaac aattaaaaat ggggtatgac
    35941 ttatggtaga caaccccaaa aaacggcagt atccattagt tcctctatcc attatccaat
    36001 ctgctctaga agaagatcca atttcaatca agatgatact caaacattat catagttata
    36061 tagctaagtt atgtttgaca aatggattta atgaggcggg gcaatttatt acctatgtag
    36121 atgagtatat gctaagacag ctagaaatta aattgataga agcgatctta aaatttaaaa
    36181 ttaactaact aaaaagtccc ctttccactt attctgttta aaagaatctt ctaatagtta
    36241 aaggattagt agattctttt aaacagaaaa cctattgatc attgaaattt aaatacattc
    36301 tatcagtacg ttaacacttc aaggagcata ttttagttgc gccatgattt cttactgtaa
    36361 agaaggagcg ataaacattc taaaataatt tttatagtca ataaaaagcg atgatttgaa
    36421 gttgggataa tgatacttcc gtaaggctcg ccgtaaactt ggagagaggt gaaattccta
    36481 tgtgcttttg acaaaagcag ctgattaaga attatttcca tatgttgatt tcaacatttt
    36541 ttctattatt tttaagacca aattcagtga ggcaaatagc atcatgtaaa tttagttttt
    36601 ctattgaacg ttctccactt tttagttttt ttatagtact caaatttaat cctgtatgtt
    36661 tagagatctc atttgcagta tggttttgta aagtttctcg gattattgtt atattagcaa
    36721 tttcattacg cataattatg ttcctttctt atcaaaaata tcaatgacta cagtgggctt
    36781 ttagtgaaaa aaaggggtac tacagtctct tttgaaaaca aaaaaataca gtatatacgt
    36841 tttctaaatg ctaagcacca acgtttacac tgtatttaag ggtttggcac ccaatgggct
    36901 gtgaggggct cgaacccgcg acccgatgat taagagtcat ctgctctacc aactgagcta
    36961 acagcccaaa ttaatacttt gttattctaa cacctaaaat aaaaaagtgc aatacttatt
    37021 atcagaattg agagtgaatt ttatggattt tcaagattat gaaaaaatat ttgaaagtta
    37081 tccatcgata ttgaaaatta ctgatttaac taaaatgtta aatattggaa aaaataaagc
    37141 ttataatttg ctgaaagata gatcaataga aaatataaaa attggagcaa ggtacaaaat
    37201 acctaaaaaa gctgtcattg agtatatatt gaaaatacaa gatgaaggta aaaatcatgc
    37261 tataataaaa tagtcaaaat ttttgccgat atttcagttt aagaggagag attgaattga
    37321 aattaagagc aaacataatt gaaaaaagag gaacattaca agtagttgta tcttataaag
    37381 actctaacgg aaaaattaga caaaagtgga aagatacaaa attaaaaaag aaaggaaata
    37441 aaaaacgagc agaaaaaatt agagatgaat ttttactaga attggaaagt caattagcta
    37501 tggaatgtga atgtgctaat ccaaatttat tgttttatga ctatttgctt aattatttgg
    37561 aagtatcaaa aaaacaagta gcatttaaca catatgttgg gtataagcat tacgtccata
    37621 atcgaattta caagtttttt tatcctaaaa aaattaaact aaataggtta aagccttatc
    37681 atttacaaga gttttatcaa tatatgttaa atgataactg tacagcaaat acggttattc
    37741 attatcacgc atttatacgc aaagctcttc aagaggctgt tattacagaa ttgataagta
    37801 caaatgtagc agataaagta agaaagccaa aaaagaaaca atttgtttct caagtttata
    37861 atcaacaaga gatcttgaaa ttacttgaca ttttgtcaga agaaaaactc tatttggtag
    37921 ttcttttaac agcattttat ggattgcgaa gaagtgaagt tttaggatta aaatggtctg
    37981 ctattgattt tttaaacaaa aaaatcatga taaaccatgt aatcatagaa aatccagaga
    38041 atatgtcaca gttaataaag aaagatcaaa caaagaactc ttccagctat cgtacattac
    38101 ctttggttga aacgattgaa cgagcattag taaatcaagc taaatggcaa caagataatc
    38161 gtattctttt aggagatgat tatattttag aagataatga gtacgtattt actatggaag
    38221 atggacggtt gatgaagcct caatatgtaa cgcatcgttt gagtaagttg attaagaaaa
    38281 atggattgaa aaaaattcgc tttcatgact tgcgtcattc taatgcttca ataatgttag
    38341 atagtggcca gaatatgaaa tccattcaag aatggctggg acatgcatcg tattcgacaa
    38401 ctgctaattt atacacgcat ttaaccgcag gagtaaaaga tcgtgcagca gaagcattag
    38461 aaaacgcatt tgggtttaaa gaataaaaaa acaaaaaaca atatctttgg ggtatcgctt
    38521 cttgttttta gaattttttt gttggcaatt tgttggcagt atgttggcaa tgaaaaaatt
    38581 cacaaactaa aaacctattt ataaagcttt ataaaacttt tttcacaaat agagcatttg
    38641 gttaagagtc agctgctcta ccaactgagc taacggcccg tacaaaaagt acttaataat
    38701 agtagcacta agaattttaa aatgcaagac tacatttaaa gaaaatcaga aaaatcttta
    38761 cttccttata ggaaatcatt catagattat tcacatttgc tcgctattat ataagcatac
    38821 caacaaacaa agttcttgca agagaacaaa ccctaacact tttttcattt tttactcctt
    38881 tcccgccgtc tccccggcgg gtctcctgtt cttaacgata aaagacaaga aggcttagcc
    38941 ttcttgtttt ttgttttcat ggaatcccgc taatcaaatc acctagttaa agcaactaga
    39001 ttgaatgttt atcataagtc tgatatcatg tttatgaaat gaaaaaagga gcggttatga
    39061 tggaaaaaat gcgtcagcaa ttaatggagt gggggaagga actagaaaca ttccgtctgc
    39121 ctcactggga agaactccct gacttagaac tttatatgga ccaagtcaga acgttagttg
    39181 atcgttatct ttcacctgtg atacaaggag aaaagcatcc attgcttacc tcttctatgg
    39241 taaacaatta cgttaaacta ggtttgatac cagcaccagt taaaaaaaga tataacaaag
    39301 aacacgtagc atttttactt gcaatcacta cgttgaaaca agtattgact attcctgaaa
    39361 tcaaagaagg gatattgttc caaggaaaaa cagtaggaat tcgtgaagcc tataatctat
    39421 tttgcgatga acaagaagca gctgtgtgga tggtcagcca attagcacaa ggtaaatctc
    39481 atccgcaaaa attcgaccag gcaacccctg tagaatatat tgctgtaaga gcggcaactt
    39541 tatcgtttgc aatgaaactt cttgcggaaa aaacgattgt tctagaaacc gaatatttaa
    39601 aagaggagaa aacaaatgaa aaacaataaa atcgcaattt tagtagattc aggtacggat
    39661 gttccagaag aattgataaa aaaatacgag atgtatatga tcccattgaa aattatttat
    39721 aaagatcagg tatatacaga taaaattgac attacccctg aagagatcta tcaacgtttg
    39781 ccagaggaaa tccctggtac atctttgccg gatggtgagt cgatcacaga aatctttgag
    39841 caaatcaaaa aagacggtta tgaaaaggta cttgcggtaa caatttccag tggattgagc
    39901 gggacatata atgtggttcg tttaattgca caacagcaag aagaattgga agcatatgta
    39961 ctagatacga aaaatattgg tattggtgca ggtttttctg cgatccaagc agcaaaatgg
    40021 ctggaagaag gagtagaatg ggatcaactg atcagtaatc tgaatgagtt agtcgaaaga
    40081 acaaaagttt tctttaatgt tgcgacattg gaatatcttc aaaaaggtgg acggatcggt
    40141 ttagttgctt ctattgtagg aaccgcttta aagcttaacc caatcatctc atgtaatgaa
    40201 gaagggattt attacacagt aggaaaagct aggggacgta aaaaaagttt ggataaaacg
    40261 gttgcttacg tcaaagaaaa aatcggtaat gcaaaggtct tcaacttagc tgttgcccat
    40321 ggggatgcga aagaagaagc aattgaaatg gaagcgagac ttaaaagaga gttcccacaa
    40381 gcaaaccaaa tctactttgg ccagatttct ccggcattag tagtccacac tggtccggga
    40441 ttattgggtg tcggtgtaca agttttagaa ggataaatca aaaaaaggaa acactgaggt
    40501 gaaaatcagt gttttccttt tttattgaag ttagaacaaa aaataataaa gtattttgca
    40561 ctttcatttt aattttctct tttgttttat aggcaaagat gaaacaaaag cagaaatacg
    40621 gtataatgag aaggtatgac aataatatat aagaggtggc tctttatgaa aaaagtttta
    40681 gttgtcgatg atgagaagcc gatttcggat atcgtcaaat tcaatttagc caaagaaggc
    40741 tatgacgttt atactgcata tgatggggaa gaagccttgg aaaaagttgc tgaggtagaa
    40801 cctgatttga ttttacttga tttgatgcta cctaaaatgg acggcttaga agttgctcgt
    40861 gaggtacgca aaacttatga tatgccgatc attatggtaa cagccaaaga ttctgaaatt
    40921 gataaagtcc taggcttaga gttaggcgcc gatgattacg tgaccaagcc attttccaac
    40981 cgagaattag ttgcacgtgt caaagcaaat cttcgtcgcg gtgccacggc tgcgaaggaa
    41041 ccagaagagg cagcacctgc ggaattgacg attggtgatt tgacgatcca tccagaagct
    41101 tacatggtga cgaaacgagg agaaacaatc gagttaacac atcgtgaatt cgaactactc
    41161 ttttacttgg cgaagcacct tggacaagtg atgacaagag aacatttatt acaaacggtt
    41221 tggggttatg attattttgg agatgtacga acagtggatg taactgtacg tcgtttgcgt
    41281 gaaaagatcg aagataatcc aagccatcca aattatttag tgacacgtcg tggtgtcggt
    41341 tactatttgc gtaacccaga acaggagtaa ttggatgaaa agaaaagtcc gcttttttcg
    41401 atcagtcaat tttaagatcg ctattacctt tattctgatt ttattgatct cgatcgaaat
    41461 tattggtgcc tactttatac gtggtttaga acgttccacg atcaatacat ttattaaaga
    41521 tatgaatcaa acggtggaat cattagcaac aacaatcagc ccggaattga atcgaaaaga
    41581 taatgcagat gatgaagagg taaatgcgaa tatcaaaaga tttattgaaa acagtgcgac
    41641 tagcgatatc atcgaaatac gggtcgttga cgaaaaaggg atcatccgag gaacaaccga
    41701 tgtcaatgaa caaagcgctg tgggaaagaa aaatgattat gttgatatca atgatttcac
    41761 gactaaacgt tatgttgctt tagataacga cgataaacgg gtgaatatca atgttcaacc
    41821 gatcctgtcc ccgaccggcg atgccgttat cggggctctt tacgtcaaaa gtaatatcga
    41881 acaaaaatat tcggaaatca ataacacagc agtcattttc tttactgcct cactaattgc
    41941 ggcatttatt tcaatgatcg tatctgtttt agttgctcgt tcgattacac agccgatcgg
    42001 tgaaatgaga gaacaagcca ttcgaatcgc tagaggagac tacagtcgga aagtcaaagt
    42061 gcatggacaa gatgaacttg gacagctggc tgataccttc aatcagttag ctgaacgtat
    42121 tgaagaagct caagatacaa tggaagctga acgcaatcgt ttggatagcg tgctatccca
    42181 tatgacagac ggcgtcattg cgacagatcg caggggtaaa gtcattacga tcaatgatat
    42241 ggctgtttcc ttgctagata tcaaaaacga ggaagcaatc ggacaatcga ttttgacact
    42301 tttagatatc gaagaagaat atacgttgcg gaaattatta gaagatccaa acgaaatggt
    42361 acttgaccgt tcaaccagta cattggaaag tgatcagatg atcttgcgag tagacttttc
    42421 tatgatccgt cgcgaatcag gttttatcag cggattagta gctgtcttgc atgatgtgac
    42481 agaacaagaa aaaacagaac gggaacgtcg tgaattcgtc tctaatgttt cccatgaact
    42541 tcgtacaccg ttgacaagta tgcgtagtta tatcgaagca ttgagcgaag gtgcgtggaa
    42601 agacgaggaa gtagcaccaa attttttgaa ggtaacgcta gatgaaacag accgaatgat
    42661 tcgaatgatc aatgacctct taaatctatc tcgcatggat acaggtaata cccagcttca
    42721 attagaatat gtcaatttca acgagttagt gaattttgtt cttgaccggt ttgatatgat
    42781 ggtgggaaat caggaaaaaa attataagat ccgtcgtgaa tttacgcaaa gagatctttg
    42841 ggtcgaagta gatacagata aaatcatcca agtagtcgat aatatcatga ataacgcgat
    42901 caagtattca ccagatgggg gaacgattac ttgtcgctta ttagaaacgc ataataacgt
    42961 gattttaagt atcaccgatc aaggattggg gattcctaaa aaagatctga accgagtgtt
    43021 cgagcggttc tatcgagtag ataaagcacg tgcaagagca caaggtggga caggacttgg
    43081 attagctatt tctagagaag tgattaaggc gcatcgaggc gctatttggg cagagagcaa
    43141 ggaaggaaaa gggtcgactt tttatatctc gctgccatat gaaccttatg aggaggaatg
    43201 gtgggaatga gaatctctga aaaagtcgtt cgtataggac tcattttgat ggttgcgtta
    43261 agcatatact tctcttatgc aatctggctg agcccagcag gaaaaagttc aatcaacgta
    43321 gatgagacaa acactcaaat gatcgagtct caaagcaatc gaaaaacttc cgaagtcttt
    43381 cttccgcttc atgttacatg gcaccattcg ggaatgatcc aagaaaccaa cagtgaaaat
    43441 ctcgtgtacc gattgcaatt gatcatcgat aaagcaagat ttggaaagct ttctgaagta
    43501 gtttcaggag acaaatcaca gtttgaacag gtgaaaaatc tgaactctgg ttttgagttg
    43561 gcctataatg cgcctttttt gctaagagaa tacaaggaag catttgattt agatttagat
    43621 gtgtcgaact tggcaaaaga aagcgaaaat atgtatttta ctagaataca attcgatcaa
    43681 ggaaataaaa aaatccgatt tttgaattat tcgaaccatc tcgtatatga agcaaatatt
    43741 tcgttgaatt ggacagatat caataaagaa ttaacagcaa aagacacaaa atggacagaa
    43801 atgacaacag aaccatcgac gatcgacacg caatatgcta cgaaacattc gatcaaactg
    43861 aagaagtata gttatatcct gtcacagcaa ccctatacac tttttagaaa tgcttttttc
    43921 cagaaaccag atagcgtgaa aaataatgat gaaagcaatg aattgatttt ttacggtggg
    43981 aatgaaacaa tgtctatcca ttcggaaacg caagtagttg attttcgtgg agagctgcca
    44041 gagaaaaaag aaaatgctag tgtattttct gaaagtttcc cctatattag caaattaggc
    44101 agttctttag ggaacctcag atattttgac cgcagtgaca atacgatcga ttatcggata
    44161 ttcgtggaag gcttcccggt ttttgggaca gacagcagag gacagattgg tgtggaagtt
    44221 ggcggtgaaa cgaatggttc tgtgcaagtg aatatccaaa ccagtttgaa tacgattcaa
    44281 gtgccgatac cttcagacga agaagtagaa cttgcaagta cgaatgaaat catccagcag
    44341 ttaaaagcta agggagcaga tagcaacaag attggttcag tgatcgttgg ctatacatgg
    44401 caaaatgtca aagaaacgaa tcgtgtcgtt gatctcttac ctgaatggta tgtgaaatat
    44461 aaagataact ggtattcagc taacgacctt ctggaaaaat tacctggatt ggaggcgaat
    44521 taattggatt tcaaaaaaat cgaatggata tttttcgtcg cttttttagg gttgaatgtg
    44581 tttttattca gcagttatca tgacgcggta taccaagaaa acaatgtcat tcgctctaat
    44641 cagactgaat caatccaaca acgtctggct agcgacgata tcacttacac ggggaagatt
    44701 tcgtctgaga acaagcaagg ctattatctt agcgcggagc agaccaatct tagcacttca
    44761 ttagcgaatt atcgcaaggc tggtaaaagc ggcttgctgg caagtggtgt gtttttagac
    44821 ggtgatgttt tgacgaaatc tttatccagt tcagatgctg aaaagaacgt gatcgatcca
    44881 gataaaatta gtagttcctt ggaagatttt ttatctaaca aagacaatat tttattcggt
    44941 aatgattatg tgtacacgaa aaacttttct catttagaag aagaaccact agaaatccaa
    45001 gcaagccaag agtatgaagg catcccattc aatgatgata cagcaaaaat cgttctttct
    45061 ttagaaaaaa acgataatga ttatagcatc acgaaataca tgcagacgca tttatctgat
    45121 atcgagcagc tgcgcgaaaa gacggaactg tatacggaag aggaagcaat caatacattg
    45181 tacatcaata acaaaatacc aagaggatca aaaattttat ggcgtcagct agcttattcc
    45241 tgtattctga aagtccggga aaaaaatgtg tatgttcctg tttggcatgt agcgatcgaa
    45301 acaagcgata aagtggtcca aattgagagc gtcaacgctt tcagcaatac gatcgtgaca
    45361 aacaatactg taccaaaagt ggaagatcaa taaaaaagcg agtataatga aaaaagttta
    45421 caaagtaaga agggaagtag gagtcaaatg gctgaagaaa aaccagcttt taaaatcagt
    45481 atcctagcaa gtggaagttc gggtaattca ctttatatcg aaagtgaaaa aaaacggctt
    45541 ttggtcgatg caggacttag cggtaaaaaa atcacttcct tgatggctca aattgatcgg
    45601 acaccggacc aattagatgg aatacttgtg acgcatgaac atcgagatca tatacatggc
    45661 gtgggtgtct tggctcgtaa gtatcatttg gatgtttttg ccaatcagca gacttgggaa
    45721 gcgatggaac cactgattgg aaatgtacca gtggaacaaa aacatctatt tgagatgggg
    45781 aaagtcagaa cattcggcga tatcgatatt gagagtttcg gcgtctccca tgacgcagcg
    45841 gctccgcaat tttatcgttt ccataaagaa aatcggtcat tcgtcatgtt gacggacacc
    45901 ggatattgca gtgattatat gcgaggtgtc attgaaaatg ccgatggtta tttgatggaa
    45961 agtaatcatg atcttgagat gttacgcatg ggaccttatc cgtggaattt aaaacagcgt
    46021 attttaggag atagaggaca tctttccaac gaagacggtg cacttgtcat gactgatgtg
    46081 atcggtaatc ggaccaaacg aatctatctt ggccatctta gcaaggaaaa caatatgaaa
    46141 gaacttgctc atttaacaat ggaaaatgtt ttgaaagaaa aagattttgg cgttggacat
    46201 gattttgaaa tctatgatac agatccagat gaagcaactg agctattttc gatttaaaca
    46261 gttcaatcaa taaaaatata aaaagcagaa gataaaatca atcagggttg gttttatctt
    46321 ctatttttat ttttttagca tattaaaaaa ataaccgtat tatcgactga ctacactgat
    46381 gtgctgcttt tcatgtttct catccagtat ctcatctact aatgcaatcg cgtaatctgc
    46441 catgctgatc gtactgtcgc tagcagcgtt tctttgtaaa taatcatcac ttaacaaata
    46501 agtacctgta cgtttgccat gtggatcaaa taaagaagct ggactgatat aagtccaatg
    46561 tagattttta gattgtttca attttaacaa tccttcgtac atattgaaag ctgttggata
    46621 gaagggtgca tttttaggta cttggtcaat catacgagtc gtcttttctg tatctaagaa
    46681 taacgaagag gcacccccaa cgacaattaa gcgtgtatcg gttccttcaa gcatctcaat
    46741 caaatgggac aaagaagttt gatgcatttc ttcttttcct ttgggagcgt tgaaagcatc
    46801 aacgacaaca tcaaaagatg caagatcagt agttgtcaac tcaaataaat ccttttgtat
    46861 aaacggaaca tctgatggca atttttctgg atgacgcacg attgccgtca cgtctaaatc
    46921 tctggacaat gcttcgtcta aaataaatga acctgcatgg ccagtagctc caatgagtgc
    46981 aattttcata aaaaacctcc ttttttattc ctgtccattg taagcagttg ctgaatattc
    47041 gtcaaagaat ccgatttttg aaaagctcag tgcgcattcc tttcatggtt cgtgatcatt
    47101 gggtatagtt aaattaagaa aactgattga taaaaataga agttgaagga gataagaaat
    47161 gaaagagtgg ttagatcgtg cctttttacc aatgataaca tctgttgtga atcttagacc
    47221 cttaactgct ttgcgtaatg gaatgactta cacgatccca ataattattg ttggctcgct
    47281 gtttttctta tttgctcatt ttcctgtgga ttacatcgct cagtggatag acaaaacagg
    47341 attttctatg tattttacac aagtatacca ggctacattt gatgtgatag ccttttggat
    47401 cgtttttgcc attgcatacg cttatgcaaa agaagaagac atcgatcgct taccagtcgg
    47461 cataacagcc ttgtctgctt atctactgct tattcagcca ctaaatactc agatagatac
    47521 tgtctggaca ggtgctgaag ggatactagg agcagttaca actgggctca tcatcggatg
    47581 gggctatgca cgtttagtaa gacttgcgaa agggtggaag caaagagaga agatacctga
    47641 aaatgtttgg cagtctttta cttcattgat tccaatcatt gttatagtca cttttacgct
    47701 tatcggttca gcggtttttc aacatttgtt cagaatgact gttgttcaag gagtatggac
    47761 atatgtccag cttcccattc aatcgttcat agacacgttg tttggtgtgc ttttattagg
    47821 tttttttgtt ccgtttttat ggttatttgg tcttcatgga tctgcgatgg tcaatgggtt
    47881 ggttagtccc gtcttgcaag cgaattcatt agcaaatgca gaaatcttag cctcaggaca
    47941 agagttgacg gtggcaaatg gcggacatat cgttactcag caatttctcg atcagttcat
    48001 gacggttacg ggtgctggat tgacgttagg ggctgtattt tttatgacgg tttttgcgaa
    48061 atcgaagaaa tacagagaat taggaaatct tgctctttta ccagcctttt tcaacatcaa
    48121 tgaatcaatt attttttcca caccgatcgt gatgaacccg atgatggctg ttccttttat
    48181 tttcgcaccg gtcgtttcag gattgattac ctattccgct ttatattttg gattcgttcc
    48241 tttatttaca gcagtccaag tcccttggac aacaccgccg atcctctcgg gtttcttagt
    48301 aggcggagtg cctgcagcga ttttacaagg tatcgtgttg tcaattagtt ttttcattta
    48361 tttcccattt ttgaaaaaaa tagattctgc aaactgcaaa aaggaaagac agacaaaaaa
    48421 tgatggagca gcagaaaaga tgaacgacta aaaaacaaaa atactcctgt cgctttagat
    48481 tgggaaaatc accaatctag gatgacagga gtatttatag tcatgtttat actagccctt
    48541 tttggacagc taattgaaca agaacaacta atgtgttcat tcctacgtgt gtgatcatcg
    48601 atgtccagat ccgaccagtt gctttgtact ctaggctgaa gaagaaacct aggaaaaagt
    48661 agattagcag atgaccatca ttatgagcaa acgcaaacag caaagagctg atcactgctc
    48721 cgacccaaaa atttgaatat cgactgatga tccccagtat actgcggcgg aatacgaact
    48781 cttccatgat gggtccagca acagttgtcg ccaaaataaa agcgggtgct tccatgatca
    48841 tctggatgat attttgtgta ttctcggaag gggttgcttt gccaaacaat acagattcta
    48901 acatgacagc gatgctttgc agaatcaatg cgacaaagat tccaaccaat ccataaataa
    48961 caatctttgg agcagaagct ttttttaccg ctgattcgat cgctaagggt tcacgttgct
    49021 taaaataaaa aatagctagg acaacggcgc ctagtatata catgactgca gttgctgtga
    49081 tcaattcaga cgtagatttt acaagtccta tcgaagctag gatcaagggt gagataaaaa
    49141 ttagaccata gcaaaaaatg ctgaggaagc tatatttttt taaagacatg taattcctcc
    49201 ttttaaggga taacaagcat gcaaggcaag cttatatcga ttgatttcaa ttatatcata
    49261 atttcaataa gcagctgtca gaataaaatt tcttttcttt gatttaaaag cttgcaaaaa
    49321 agagggagaa tgagtattat taaaaatgta ggttagcact cgaaggttaa gagtgctaac
    49381 agattaaaaa aagagttatc ggagggattt atcgtgttaa aaccattagg tgatcgcgtc
    49441 attatcgaag tcgcaaaaga agaagaaaaa actgtcggag gaatcgtttt agcttctgct
    49501 gctaaagaga aaccccaaac tgggacagtt gttgcagtcg gtgaaggccg tttgttagaa
    49561 aacggtgaaa aagttcctgc cgctgtaaaa gcaggcgacc aagttatgtt cgaaaaatac
    49621 gccggtacag aagtaaaata cgaaggaaaa gaatatctga tcgtcgcagg aaaagatatc
    49681 atggcgattg ttgagtaaat ttggaagata aaaaaataat caaaaataat caatgaggtg
    49741 aatcaatcat ggcaaaagaa ttgaaatttg cagaagacgc acgcgcagct atgttacgcg
    49801 gggtagataa attagcagat acagtgaaag taacattagg acctaaaggc cgaaatgtcg
    49861 tactagaaaa atcttacggc tctccattga tcacaaatga tggtgtgacg atcgcaaaag
    49921 aaatcgaatt agaagatcat tttgaaaaca tgggtgcaaa acttgtatca gaagtagcat
    49981 caaaaacaaa tgatattgca ggagacggta caacgacagc aactgttctg acacaagcaa
    50041 tcgttcgcga aggtttaaaa aacgtaactg caggtgctaa tcctctaggc attcgtcgcg
    50101 ggatcgaatt ggcaacaaaa gcagcagtcg aagaattgca taatatctca actgttgttg
    50161 attcaaaaga agccattgca caagtagcag ctgtttcttc gggttctgat aaagttggac
    50221 atttgattgc tgatgcaatg gaaaaagttg gaaacgacgg tgtcatcaca atcgaagaat
    50281 caaaagggat cgaaacagaa ctagatgttg tagaaggtat gcagtttgat cgtggttact
    50341 tatcacaata tatggtaaca gataacgaca aaatggaagc tgttctagaa aacccatata
    50401 tcttgatcac agacaaaaaa atctctaaca tccaagatat tctgccattg ttagaacaaa
    50461 tcttgcaaca atcacgtcca ttgttgatta ttgctgacga tgtagatggt gaagcattgc
    50521 caactcttgt attgaacaaa atccgtggaa cattcaacgt agtagctgta aaagctcctg
    50581 gtttcggcga tcgtcgtaaa gcaatgcttg aagatatcgc tattttgaca ggtggtacag
    50641 taattactga cgatctaggt ttagaattga aagatgcaac aatcgaaaac ttaggaaatg
    50701 cttctaaagt tgttgtagat aaagacaata caacgatcgt agaaggttct ggagaaaaag
    50761 aagcaatcga agctcgtgtg caattgatca aaaaccaaat tgctgaaaca acttctgatt
    50821 ttgaccgcga aaaattacaa gaacgcttag ctaaattagc aggtggtgta gctgttgtta
    50881 aagttggtgc agctactgaa acagaattga aagaattaaa attacgcatt gaagatgcat
    50941 tgaatgctac acgtgccgca gtagaagaag gaatggtctc aggtggtggt acagctctag
    51001 tgaacgttat cagcaaagtt tctgctgtag aagcagaagg agacgtggca acaggtatca
    51061 agatcgttgt tcgtgcttta gaagaaccaa ttcgtcaaat cgctgaaaac gctggttatg
    51121 aaggatcagt gattgttgat aaattgaaga acgtggaact aggcactggt ttcaatgctg
    51181 ctactggaga atgggtaaac atggttgaag caggtatcgt agacccaact aaagtaacac
    51241 gttctgcctt gcaaaatgcc gcttccgttt ctgccttgtt attaacgaca gaagcagtcg
    51301 ttgcagataa accagaacca gcagcgccag cagctcctgc aatggatcca tcaatgatgg
    51361 gcggtatgat gtagtaatta agaaagaacc ctattttaac agggttcttt cttctttttt
    51421 tgttttattt taagcaaaag gggcaaagaa ggggcaaaaa tatcaaaggc tatttagttt
    51481 atcaattact tgttgtttgg ctctttttgt tacatggtta taaatagaca gagttgtgtt
    51541 tgcatcagaa tgccctacac gctccataat ggctttaaga ggtacgccta attcagataa
    51601 tagtgaaacg tggctatgtc tgaatatatg tgaggataga cttttttcta attccaactc
    51661 ttcctctact ttatggagta tagcgttaaa tgagtgcagc gcaagcggag tgccacttgt
    51721 agatataaat atatattgat cgggatctgt gggtctacct gcaagaatat tgtcagctat
    51781 cacactttca atcaattctt ttgcacgatt gggtaattgc acttcacgtt gcgaataagt
    51841 attttttgga gttgttttta tagcattatc cattttcaca gatgtgtaat ctaaggtccc
    51901 attaatggaa attttcccat cctcatagtc cttcatttgc aaagctagca attccccata
    51961 tctcaaacca gttaaatata gaaactcagc tattatgccg tgtagttttc tgcgaggatt
    52021 ggagtatagc tgtttcagta tttgatcaat ttcttctttc tccagatatt ttttattcat
    52081 agaaagcctt cttttttctt cttccacttt tttaggatgg attttaactg ctagcgcagg
    52141 gtttctttga atgtattttc tatcgattgc atagtttagc ataacagata gagttgtttt
    52201 tgtttgtttt gtgtagttca gtgagaggtc accaaacgta tacatatctt caattatctt
    52261 attaatgagt gtctcatcaa tgtttctaac gatcgtatca tcgcttatgt gcttagaaac
    52321 atgtttcatc atcattggaa ccttcaaata gctagtacgt ttaacatgct gcttataata
    52381 ttcataccat tctttataca gctcaccaaa agtgatatct gatttattgt aatcttcgag
    52441 tgcttcttta atttttttat ctagaatttt ctgagctttt ttccacgctt gtggtgaatt
    52501 acttgtaagt gttgtagatt tttttcgtgt tttttctgta taaggatcta catatctttc
    52561 aataaacttg aattggccat ctttggtctg ttcaacccac acttttaaca tctcctatca
    52621 tttgctataa taggcataac aaatagacct atataggttt gttttctaaa agcacgctct
    52681 tactctggac ggtggggcgt gttttttatt tattatacag ttgctttaaa aatagctgct
    52741 ttttcaaatg atactaaagg agagaagtgg atttctattt ctcccttttg gtttaacgtg
    52801 aaatgagcag ttacatccat ttgttttcct ggagcaacag atcccattgt attttcgttg
    52861 gcatatgtct ctgatttttt gtcatctggt ccatatactt ccacatctgt acctacaggg
    52921 atatctgaat cgccatcatt ttttacggta taagtaattt ttactacttg tgcaggttgg
    52981 ttttcttcaa attgatttct ttcatcagtt agttctacac tatttagtgt atattcagca
    53041 tcaccaactt ttacagtgtc accaatctta tagaaggtat cgcttttttc ttctttagaa
    53101 gatgaagcag aggttgaaga tttagttact ttttcgccac cattatcact tgctttatta
    53161 ctatctgatc caccatttaa agcagaacca ataattataa ttaaaattac tgctaatacc
    53221 caaaaccata cttttttgta aaaaggttta ctaactttat acatttttcc gtcttgaccc
    53281 ataacttttt ttgccattta aatattcctc acttcttgtt ataatatatt tgcgatctca
    53341 gaaatgaggt atgagtccgt gtgccagcac gggctttttt aatgttttgc tgaaatcggt
    53401 ttttttaata attcttgatg tattcataca tattgccttg cgtaagaata ttttttctta
    53461 aaatagcatt ggcagacagc ataacaagtg tatctgagct tattctgaat agaataatac
    53521 tccataaatt tttcgagatt aaactgagat tcatcagtca gttcgttctc aatatagata
    53581 tttaataaaa ttagaatagc tattttatct gcttctgttt caaattttga gtgaaaagtt
    53641 gtagaagtat cgtacaaaac tgaatattca aaatgagaag cggtgaaatg cgcaagttca
    53701 tgagataaat gaaaggcttc tgcagtttcg ccatatagat tttcattcaa aaaaatgatt
    53761 ctaggcttcg gataatagaa gccaggctct ttcatttcca tgtaaactac tttcaaattg
    53821 tattcgctca acatttcttt caatttcaaa tacatacaac ccatcactcc aactattcat
    53881 tttcctctaa agctttagca attgcaatcg ctttacgcat tgtctcctta gatatttctt
    53941 ttccatcaaa agaaaaaaca gtatcgtttt ctgataaatc cacatgttta ggggtttctc
    54001 tttcttctct acctagaagg tagtctacag agactccaaa gtaatcagct aaccgagcta
    54061 actcaataga attaggagtt tgtcttttcc aacttcctaa atatccattc gaatacccga
    54121 aattcatttc gagctgtcta atggataatc ctttttcttt tgctaactcc tttattattt
    54181 cgtaagtatt cattgataaa tcaacctttc tgaatgctta caaaaaaagt ttagataaat
    54241 aatgtaaaaa tagttgactt atatacatta ttaatctata ctatgttttg taaacaagtt
    54301 taacaactaa aaagacaaca aaaaatacta ttgattaata aatgccaacc gccaagaaag
    54361 ctttttaaat caaagtttat atgtcttatt taactatgca tttagtatag attattaatc
    54421 taataaagtc aacgcagttt tttaaaaagt tgttaaattt gtttacgaat ataaaagaaa
    54481 ggagagaaga atatggaaaa aacagtctcg gcaaaaatcg aagatttgaa attagacata
    54541 ctgaaacaag caaaagtggc gatggaacac gcggtagata aagaagactc tgccatggtt
    54601 gcagccatag cagagatttt agctcacgtt tagtcatcgt ttttacggtc ttctggaagc
    54661 tgaccataga taacagcata atgaatatct aaataagctt gaacaatttc ttttggcgaa
    54721 attgattcgc ctttagtaga tacttgtgat tcgtgattta gccatgcaac aactaaatcc
    54781 gcagcgattt caaacggcgt tacactttgt gtcagttgcc ataaagaatt tcacaatatg
    54841 tatggtcgtg gtaataatac caaagagcaa tttgaggaat ttatggaaat taaaaaatac
    54901 aaaaaatcat ttattaaagg aggctaaaca atgaacaagg aaatgagcca aacaatcaag
    54961 gtacaaaaaa tgatagatga tctaacacat ggaattgata gccaagccga taaaatcatc
    55021 aaggaattgc aaggacaaaa agttaaggat gcaaaaatgc ttctcaagac tatcaacttt
    55081 gaaatgaatc caacaaaaag aaagctcgcc gatgtgttgg aagaaaagtt ggcttccgct
    55141 atcaacgagc aagaattact atttgaaacc gatactttta acagctgatg ttttatagat
    55201 gacagtttca tccgaatcta tatcatcgcc gagagaaaag aatgttgctt ttgacaaaaa
    55261 gccgataaga cctacaagcg gtcttgatgt tgcaagagta gataccccaa tatcaccttg
    55321 caaggtattc acgtaaattt gagaagaata gaacattcca tctttttctt cgatatttga
    55381 cgcatcatcg tatccaacga ccaaagtatc ggcaaatatt ttgatttttt ctccatcaag
    55441 catagtaatt acaaattctg gtttcataaa ttttcacctc cttaacaatt atttcagcct
    55501 gtcacactga taaggaaatt ataccaaaga aaggaatgag aaatatgaat acaccacaaa
    55561 tttttaattt cgagcaaaac gaagttcgaa ctattttagt aaatgatgaa ccatattttg
    55621 taggaaaaga tgtagcagac gttctaggat attcgaatcc gcaaaaagct attcgagatc
    55681 atgtggactt ggaggataag acgcagaacg attcgttcac cgtcaatgga acagcagttg
    55741 ttttgattaa cgagtcaggc ctttacagtt taatcttaaa atcaaaactt ccctctgcca
    55801 aaaaatttaa acgttgggta acgagtgaag tccttccaac aattagaaaa acaggtagct
    55861 attcaaacgt acctcaaagt tttgcacaag cattgcgttt agcagcggat ttagaagaaa
    55921 agaaccaatt actcgaacaa caaattgccg agtacgaacc aaagattagc tacttagata
    55981 cgattctttc atcgacagat acggtagcta cttctcaaat tgcagctgat tacggaatgt
    56041 cggcaattgc tctaaacaaa ttgctaaacg agttaggtgt tcagcataaa gttagcggac
    56101 aatggatact ttaccgaaaa catatgaacc aaggatacac aaaatcgcac acaagtgaga
    56161 taccgaaagc cgatggcggc actaaagttg taatgaatac caaatggaca cagaaagggc
    56221 gagtgtttat ttacaactta ttaatcgcag agggctatta ccctcaaatg gatttagagg
    56281 aaattggtta gaaaggagtt ttagtatgac tgacattgca gaaatcactc aacgagatag
    56341 agaaaaaatc aaagaatatg tcgaaagttc aaagttctta acttacacca tgcttgctga
    56401 aagatttgga attagcaaaa gctacttatc tttaatttta aacggtaaaa agacttctgc
    56461 agaagcaaac agaattatag attcgattat cactatgtac gaattgtaga gggaggtaaa
    56521 gtcgttggaa gataaaatca ttcttacaaa atcagagctt caagaaatgt taaacatcga
    56581 gtatggaacg ataattttta ggagggataa taagtgaaaa aaccaacact ttcagagctg
    56641 atagaagctg ctgagaaggc agtaaaacca gacgactggt accgacaaag tttaatcttg
    56701 gagaagttcc acggcatgtc aaaaactact ttagttgaat actgcaagga aatggaaaca
    56761 attcctgaat ttgcagaagg aatacttcgt ccaggtcact caaccacatt tattcattac
    56821 catactttta tttggttttt aaagtggaaa gacgcaaaca aatatcgtgt aaaaagttta
    56881 tcgccttcag atgttttgaa ggaggcaagt taatgggcaa attcaacaga gcactagtat
    56941 tcagcgcacc gctaatcatc tacgctttag gcctctgggg aagcagacaa gcgttgatag
    57001 ggacaatcgt ttacatggtt tggattttta tggggcttga tgaagctgag cacagagcga
    57061 aaaaagccag tcgggaggga ctgactaaaa tatgattttt ttatttttgt tcctttgggc
    57121 tgctattgcg tacttcatcc agctccttgt tgtcaaagtt gctcttattt cttttaggaa
    57181 gaacaaaaga aaatagcgct ataaatgaaa gataagtagc cgctacatta aactgttctt
    57241 ttgaaaaagt tacagtcatc ggaatgataa ttgatattga ggaagtaatc ttaattaaaa
    57301 atatagcaat attttcaaca tcgcgttggt ttttatttaa cattgtccaa catgttttag
    57361 caacaataaa aataattaca catgatatta gcacacagac tgaaaaaagt tttagaaatg
    57421 cgatgtagtc agtgttattg aactccttca aaatactatt tttattcaat gtaataaaag
    57481 gaattttttt aattgaagac aggaaagata aggtaataaa cagaattata tatatggtta
    57541 aagtcgcagt aagaatgtaa ttgagctttt tcctcataga atcacctcac tttctaacga
    57601 aaattatacc aaaaaggaga gaagaaataa tgcaagaatt agtaattttg aaaaataaag
    57661 aagctgtaac tacgagctta caagtggcag aaagtttcaa aaagaaacat aagcatgtac
    57721 tagaagcaat tgaatcaata aaaagatcgg tcgaaaattc ggccaatgtt gaagatgggt
    57781 ccaattttgg acagatgttt gtggaaggga acgagccaga ctcatacgga agaagtcgga
    57841 gagtttattt catgaataga gacggatttt ccttgctagc tatgggattc actggaagta
    57901 aagcaataaa tttcaaacta aaatttattg aagctttcaa cgaaatggaa gatgttattc
    57961 ggaagaatac tgttcctcaa acaattgaag a
//
LOCUS       DACEEZ010000007        56574 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20313_2017
            SAMN12639966-rid9879223.denovo.007, whole genome shotgun sequence.
ACCESSION   DACEEZ010000007 DACEEZ010000000
VERSION     DACEEZ010000007.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639966
            Sequence Read Archive: SRR10023158
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 56574)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 56574)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741683.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741683.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:44:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,016
            CDSs (total)                      :: 2,962
            Genes (coding)                    :: 2,889
            CDSs (with protein)               :: 2,889
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 36 of 73
            Pseudo Genes (incomplete)         :: 29 of 73
            Pseudo Genes (internal stop)      :: 18 of 73
            Pseudo Genes (multiple problems)  :: 8 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..56574
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639966-rid9879223.denovo.007"
                     /isolate="E20313_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            336..1439
                     /locus_tag="IWO89_000491"
     CDS             336..1439
                     /locus_tag="IWO89_000491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactate oxidase"
                     /protein_id="HAQ0455274.1"
                     /translation="MEKTYQAGTNEGIVDFINMEDLEIAASQVIPAGGYGYISSGAGD
                     LFTYQENERAFNHRLIIPHVLRDVELPDTTTHFDEEMLTAPIIMAPVAAHGLAHVKAE
                     KASAKGVADFGTIYTASSYASCTLEEIREAGGEKAPQWFQFYMSKDNGINLDILEVAK
                     RNGAKAIVLTADATVGGNRETDRRNGFTFPLPMPIVQAYQSGVGQTMDAVYKSSKQKL
                     SPKDVEFIAAHSDLPVYVKGVQSEEDVYRSLESGAGGIWVSNHGGRQLDGGPAAFDSL
                     QYVAEAVDKRVPIVFDSGVRRGQHVFKAIASGADLVAIGRPVIYGLSLGGSTGVHQVF
                     DFFKTELEMVMQLAGTQTVEDIKKIKLRENRFI"
     gene            1723..2403
                     /locus_tag="IWO89_000492"
     CDS             1723..2403
                     /locus_tag="IWO89_000492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WxL domain-containing protein"
                     /protein_id="HAQ0455275.1"
                     /translation="MKKNMIGTLLLSTLLLGGLATPAFAEGQATSKGDITFTEPTNTV
                     EPLNPTDPSKPVEPADPENPATGQTGSLTLDVVPELPFGTHEIESGTKTYQVDASKND
                     TPYLQVSDRRGVGADGQAQGWNVTVSVSDFVNGSQVLQGAELDFGTSTVKSTSDNEST
                     GPTSQTVTGLSKASAATPIFTAAKDQGLGTWLSVYDPANITLKVPKAAAGTFTADLTW
                     NLVAGPVA"
     gene            2493..3527
                     /locus_tag="IWO89_000493"
     CDS             2493..3527
                     /locus_tag="IWO89_000493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF916 and DUF3324 domain-containing protein"
                     /protein_id="HAQ0455276.1"
                     /translation="MNQSKRLFVSFFSILSLFFIFPSISQAKDSAGDFGIKPVFPENQ
                     IDKAIGYFDLLVAPEQNQTLEVIISNSSDEERTFEVSVNPAVTSDGGTIDYSQKNPTL
                     DETLPFDVRDVLLIAKKEINVSAHAETTIPIEVKIPAKSFKGRVLAGIHVSPKEEAET
                     ENATEGAQIKNRIAYNLAVVLQESQETIEPDLKLLSGDLDEVNAKPTVQLRFQNPQPR
                     IISNLIFTSKIFYENQLYIENTSNEFLVAPNSNFHLNLDLAGDKAKAGDYRAEIIAKS
                     GDSNEWRFTQNFTIKKEKAQKVNENSVFAVQEQSFPWLYAGLVAAVLVLWLILLFLWV
                     HKKKKKGETT"
     gene            3541..3876
                     /locus_tag="IWO89_000494"
     CDS             3541..3876
                     /locus_tag="IWO89_000494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0455277.1"
                     /translation="MKTSILLGGIIASLLFFSWQEPVQADTKSSGTITFYDLSPVPHP
                     SGSVNGASKQQTQLPIYKESNQHGVFPETNEEKIRFLYLIIGIELWLLAWLLLWRRKE
                     KEDEHVATK"
     gene            3860..6340
                     /locus_tag="IWO89_000495"
     CDS             3860..6340
                     /locus_tag="IWO89_000495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0455278.1"
                     /translation="MLLQNKFSFFRSFFFTSLFVLSIFIFSPSAEAVGATPPAISYDP
                     YTYENIVPPNGGRSAVNGGKLVQLTGANQLPKLTATYLNDGSNDPWKGGTVRFHWESH
                     VKLTNVLDLVNITAQGFAFVAIINLPEHVSSSDVLKAIDWNNAYLYIAGAPIKVTSGS
                     LLSAGSHTLRFGMGSWDTNTLLADIIAIITRGEFNVDDIPLQFDVTANVAKMTENGDK
                     YDTSPNKILTKGKLQPSMSKKAVFSVDFYDSNNIIQDRMFNSILAPAAGRFYPKLTGG
                     NLNPGAYAFSASAEINTWNSYISTSDSTGKYNTFADTTEYVDGNNQNLPGSTNSRTLT
                     MGLTEGRFDQLPFSRFDRVVNYFTGKNVTQGAILTHTPASTSGLNQTANIVYSGKDAQ
                     GGLLSPVALKVLEQYKLDGTVLPTNLTADSQWGKLLSSASYQVTANWSATELKKGSIH
                     YRLYSKGTQKLATGWTDQLFQTIQSNNGGVNTATSTLPALASGQYYFDYRLVDDVLTN
                     MYPSFAYKWQSEQAGITTLPQITVANFPSVSADSQLKNLSRQPETGDPLSALSGDTIQ
                     ENVTFTLTKSGDAITDKKITISLPENTTYINGSLKLNGTTIADTGIQQGVSVPADLLS
                     KIGDTIHLTYNYQLNTVDTSVQSVSILTKAAVLSSNITLADGAKLPNPVVQTSAKTIL
                     VPKQELTLVNVPDDFTFGNDLPKPLKTSYYEAKGDFSFDVRDTRLPSTSPWQLTGTLT
                     SLFKNDQGQELSGTKLYFNHSGSKQLIQQGQNTLIYESDGTAKGEVLVDFPDTDGLLL
                     EVNSSTNAQPGATYQGMVTWELTAGPTS"
     gene            6396..7955
                     /locus_tag="IWO89_000496"
     CDS             6396..7955
                     /locus_tag="IWO89_000496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAQ0455279.1"
                     /translation="MRRKLLLLKSEMLGGVDLELFSLLEKNEQRQIKLFQLLLEEKGE
                     ISVNNVLTRLSVDRATLKEDLFYLQANLENIKQLEIVIQEGNILLIRHGNIAAVDVYY
                     HCYLRYSTKYQILLYLLENGSFDRQKLVMELNISSATLSRRVKELNDSLNEFHLKIKS
                     GRLTGPESQICYFYFQLFWFGRPYLENKKEYSDLSGNTFLDLLQKEFSFTITEEGRLK
                     FEIWLSIMKKRMRCTKKSKPDYSNLPVGLFQTTQFLLDLHTFLTRYFFQYAFPWNQNE
                     TLVFYLFMISNFTLDTHNSHVQEFLISEKYKDDVVAQLNRTFKQSIEEYFSFYVFPEE
                     ISQKIALTVMQNHAHFAYFRGWMNIFGQEGLKQRLTEVEDKQFLVLCERLVRQTFAIV
                     DFTEVDEENARFELFARYASILRTLIKQLELRLQIAYDLPYERLMADIVKEIIQEQLL
                     NTIKYDLVEYEKEKQYDIILTSQLKEYPSQKSAKVFVFTSNEYNYDFPYLNQFLKECY
                     LEKLNLRMNKT"
     gene            7952..9316
                     /locus_tag="IWO89_000497"
     CDS             7952..9316
                     /locus_tag="IWO89_000497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HAQ0455280.1"
                     /translation="MKIVIIGASHAGITAALNLRKLQPKAEVLLIDEDHKDGLGYVSN
                     GINLYLKGKIRSLAEVAHNMRTLQESGAKLITEWRVTELDPDKHQLILASKEGKNETI
                     TYDKLIVATGSSPVTLYKQIEAENVYTYKNLVQSKQVLAALKEAKEVVIFGAGYIGLE
                     LADALRNKGYIIHLVDYMPNVLSRYFDKDMINSFQNQLQTKQINFYPNEFLIDWKKSE
                     EKVVSVQLLSQAIKADMVIFSAQTRPNTTLLKEKVALYEDETVIVNEYLQTSDPDIYA
                     IGDIVPVSFDKNKRHLFLPLVTRAVHMARAVALTLSGQPTAYDLRQKITAAVIIDYFL
                     GTVGLTEDEAPFLEQNTGSCSGEFDLFPQYDEENKTVNAKLIYHPDTLEIIGGQLISQ
                     EFLLSDLNLLADIVKHKTTIPQLAVEDFGFLSEHTSRFHYLNELAFKVLAKRANSNRV
                     DKRL"
     gene            9487..10557
                     /locus_tag="IWO89_000498"
     CDS             9487..10557
                     /locus_tag="IWO89_000498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAQ0455281.1"
                     /translation="MFLALNEIMHSKLRYALVAGVMFLIAYLVFFLTGLAYGLAQDNR
                     TAVDKWEADSIVLSKDANSNLGMSMITKKIAEEVEGGKVAYLAQTPGVVTSKDSTEEG
                     KINVSFFGIDKNQFIMPNLVEGKAFDNDDEAVGDISLKEEYGLAVGDTVKLSGSDKTF
                     KLTGFTDHAKFNVSPVLYTTINAYQEIRFEKEDTSENARINAIVVRGKISNLPEDLEQ
                     IKISKFINELPGYNAQVLTFGFMIGFLIVIAAIVIGIFIYVLTMQKINIFGVMKAQGI
                     TGGFIARSVVAQTFILSFVGILLGLLGTVGTSLVLPDAVPFQSNWLFFGVISLLMLVV
                     AVLGALFSVRTIVKIDPLKAIG"
     gene            10572..11240
                     /locus_tag="IWO89_000499"
     CDS             10572..11240
                     /locus_tag="IWO89_000499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ0455282.1"
                     /translation="MKAIEFKEVEKSFQDGDQVVKALKETTMSIDKGEFVAIIGPSGS
                     GKSTFLTLAGGLQTPSSGSVFINDQEFSNQKESERVKLRFKEIGFILQASNLVPFLTV
                     KKQLLLVDKIKHEHRQKEAKELFEQLGVDKLINKYPEELSGGERQRVAIARALYNDPS
                     IILADEPTASLDSEKAREVVDILAKESKDKNKATIMVTHDTRLIDKCDRIFMMEDGVL
                     KEKE"
     gene            11260..11838
                     /locus_tag="IWO89_000500"
     CDS             11260..11838
                     /locus_tag="IWO89_000500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HAQ0455283.1"
                     /translation="MVHRYNKDTQQKILKEATDLFMSKGYLGTSTREIAQKVGITQPN
                     LYHYFGDKEKLYTAVLENHLKDVGKALREIVQTSETGFQVTLTKMAQFLIETHLVDLF
                     LMLHDLESNLSKETRDHLFFLWKKNYREPFEEIFSANQSVLRNGISKEIAARHFFLVL
                     APYITRSKRPVEEPLNVAQLIDLYLNGVLGEA"
     gene            12241..13152
                     /locus_tag="IWO89_000501"
     CDS             12241..13152
                     /locus_tag="IWO89_000501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HAQ0455284.1"
                     /translation="MKKIIGAFVFAIIIIIICFQIDVFGQKKIVGQTVSSTNSTVTKT
                     KESFDDTEQETLIKIGKNDRTLYTDDSLTTPLATINGGELAEFLSETKNAYQIKTNDG
                     YTGYLALEDGTKVTKNIQTKPEELSDAVIVLDPGHGGNDTGALSNDEQTEEKDITLST
                     AIKVKKALEDAGATVYLTHTTDELVQLGDICDYSEEKKADVFISLHADSTEYANEATG
                     ITTYYYYGQEETLAQTIADSFTDLPLDSRGISTGNYQVLRENLQPSILIEMGYMNNDS
                     DLKELVTDSYQQQIAASLTQALTTYFQ"
     gene            complement(13408..14325)
                     /locus_tag="IWO89_000502"
     CDS             complement(13408..14325)
                     /locus_tag="IWO89_000502"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HIII"
                     /protein_id="HAQ0455285.1"
                     /translation="MSNVIIKATNDQLSQMKAYYRSYLLNKQIPYTSFAAKKDGTTIT
                     AYTSGKVMFQGTNAAIEADKWGEAQDKQAKPAKTASSLPKNISQLSVLGSDEVGNGSY
                     FGPLTVCAAYVKRDQLAELRKLGVRDSKELKDSQIIQLADILKKTIPYKLLVLSPEKY
                     NEIQPKYNAVRMKVALHNQAIHLLLKKIEPEKPEAILIDQFTPEANFKKYARLEKNQL
                     QEKLYFVTKGEQYHLAVAAASIISRASFLEELHKASAEAGITLPSGAGTKSDQVAAKL
                     LEKGGMPMLEKYAKLHFANTEKALKLLKK"
     gene            14527..14964
                     /gene="zapA"
                     /locus_tag="IWO89_000503"
     CDS             14527..14964
                     /gene="zapA"
                     /locus_tag="IWO89_000503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapA"
                     /protein_id="HAQ0455286.1"
                     /translation="MAHEKTRYKAVIANQTYTIIGRETKHHMDIVTKLINEQLAELKQ
                     LSPQMDNEQAAILMAVNALSDQLKKQERILELEEETAELKKKMIKFTELENRVKRIEA
                     IENEAREVLKENGQADHTIKNHVEAQQILNEKRKGQIKQKSSN"
     gene            15022..15569
                     /locus_tag="IWO89_000504"
                     /pseudo
     CDS             15022..15569
                     /locus_tag="IWO89_000504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629130.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="CvpA family protein"
     gene            15684..18044
                     /locus_tag="IWO89_000505"
     CDS             15684..18044
                     /locus_tag="IWO89_000505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease MutS2"
                     /protein_id="HAQ0455287.1"
                     /translation="MNNRILETLEFEKVKQMVRQFVVTAQGKEELAELVPVSEKQTIT
                     NWLQETEDGLKVQRLRGGIPIPKLENIRPHMKRIEIGADLNGVELAQVSRVLSTTSEL
                     NRFIDDLSDSEIEFARLYMWADQLVTIPVLSRRLKEAIDEDGRVTDDASPELKSIRQN
                     IRRSEQAVREQLDGIVRGKNAKYLSDAIITMRNDRYVIPVKQEYRGVFGGVVHDQSAS
                     GQTLFIEPKQVVDLNNRLRQYQIAERNEIQRILSELSAELVPHRQEIIHNAYVIGKMD
                     LMNAKARFGKEVKAIVPGISEDNHVVLKQARHPLIDQEKVVSNDITIGKDYQAIVITG
                     PNTGGKTITLKTLGLLQLMGQAGLPIPAGEESQIGIFEEVFADIGDEQSIEQSLSTFS
                     SHMTNTVDILSKVNEKSLVLFDELGAGTDPQEGAALAIAILDDLGKKSAYVMATTHYP
                     ELKVYGYNRANTINASMEFDVDTLSPTYRLLIGVPGRSNAFEISSRLGLDTEVIDEAK
                     QLMNDESQDLNEMITDLENRRKMAETEYLEMRHFVSEAQELHDDLKEAYSYFFEEREK
                     EMEKAKKKANEVVSEAEEKAEKIIADIRKMQQQIGQGNVKEHQLIDAKTQLANLHQEE
                     TLKKNKVLKKAKEQKTLKPGDEVLVTTYGQRGTLLRKNGNQWQVEIGILKMNVSEDEL
                     TPVAPQKEPTQRVIHAVRSESSSHVPNQLDLRGKRYEEALSEVDQYLDSAILAGYPQV
                     TIVHGKGTGALRKGITDYLKNHRSVKSFEFAPANQGGNGATIVKFK"
     gene            18131..18445
                     /gene="trxA"
                     /locus_tag="IWO89_000506"
     CDS             18131..18445
                     /gene="trxA"
                     /locus_tag="IWO89_000506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HAQ0455288.1"
                     /translation="MSQVITDKTFNEETDKGLVLIDFWATWCGPCRMQAPILDQLEQE
                     YDEEEFRIAKMDVDENPETPQQFGIMSIPTLMLKKDGQVVEKAVGVHSKEQLRQMIDQ
                     YL"
     gene            18627..20405
                     /gene="uvrC"
                     /locus_tag="IWO89_000507"
     CDS             18627..20405
                     /gene="uvrC"
                     /locus_tag="IWO89_000507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HAQ0455289.1"
                     /translation="MNERIKNKLALLPDQPGCYLMKDKNGTIIYVGKAKILKNRVRSY
                     FTGSHNTKTERLVSEIEDFEYIVTESNIEALLLEINLIKKNDPKYNIMLKDDKTYPFL
                     KITNEKYPRLVITRKVLKDKALYFGPYPDVGAANETKKILDRLFPLRKCKPSQTKEPC
                     LYYHLGQCLCPYYFDVDPKVYTKIVDEVKKFLNGGHEKIESEIHQKMEKAAENMEFEK
                     AAEYRDQIRAIETIMTRQKMTNTDLLDRDVFGYAIDKGWMCVQVFFVRQGKLIERDVS
                     MFPFYNEAEEDFLTYLGQFYQENEHFIPKEVLIPDTIDKPSVEALLATKVLQPKRGEK
                     KKLVELANKNASVALNERFDLIARKQERTIGAVERLGNAMNIPTPIRIESFDNSNIMG
                     TNPVAAMVVFIDGKPARKEYRKYKIKTVNGPDDYASMREVIYRRYSRVIREGLPLPDL
                     ILIDGGKGQVDVAKDVLENQLGIDIPIAGMAKNDKHKTNELLFGIDLAVVPLERNSPE
                     FFLLQRIQDEVHRFAITFHRQLRSKNSFASRLDEIEGLGPKRKKMLLKEFKSLKNITA
                     ATTEELQEIGLPKNVAQNVFDKLHQE"
     gene            20550..21449
                     /locus_tag="IWO89_000508"
     CDS             20550..21449
                     /locus_tag="IWO89_000508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ0455290.1"
                     /translation="MLEVKDLVKTFGNYTAVDHVSFQIPDGKIMGLIGQNGAGKTTTF
                     RLILDFLTQDQGEVLWNGHHLGEKDYDVIGYLPEERGLYPKVTIQDQLLYFAELRGKS
                     RKEIEPKIDFWMEKFQVKGKKTDKVKSLSKGNQQKVQLIATLIHEPKLIILDEPFSGL
                     DPVNAELLKDGILELKKHGSCVIFSSHNMDNVEKICDHLIMLRNGKMVLNGKVHEIRE
                     SFGRTKLFLESGLSQQEVEEIEGVKKVILHEDNTLEITLEDPEAGKEIFTRATQFGYI
                     PMFNQQPPTLEEIFKLKAGEIDA"
     gene            21442..22704
                     /locus_tag="IWO89_000509"
     CDS             21442..22704
                     /locus_tag="IWO89_000509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAQ0455291.1"
                     /translation="MRKFWIITKDVYLKNVKSISFLIMILVPFIMLGIIYVAGNFAQQ
                     NSDVNKIGVLADNEQISQQMSQVKTDDFEFKAVDSEEKAQKELSEEKIDAYLVVSTEN
                     DKINGMLYSENSLGQSTQLLIQQQLTGLQSMLRASQLGISPEEVASLSQQASLTRQKV
                     NFDSKGKMTVGEDNSTVQYVVSYVATIVLFIIILTYAQIIAQEIASEKGTRIMEVILS
                     STTAQKHFYGKLTGVLLVALTQMALYGIIFGIGYSQFKDMEMVKNILDGISLDNLIGP
                     FLWYSLLFMIFGILIFSVLAALCGSLVNKAEDTAKAILPVTYLSLGGYMLGLILGASD
                     PNNIVIRITSYIPFLSSYIMPVRLANETVGMGGALISLLVLVAVTFLLMILSANMYKS
                     NVLVYSEGGLWSSLKQSISIMRNERKKA"
     gene            22867..24765
                     /locus_tag="IWO89_000510"
     CDS             22867..24765
                     /locus_tag="IWO89_000510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-bisphosphatase class III"
                     /protein_id="HAQ0455292.1"
                     /translation="MDRYLDLLSEKFPTTEDVITEIINLEAILQLPKGTELFLSDIHG
                     EFPAFDHILRIGSGNLKEKIKDLFSEQMTETEISQFTIFTAYPEYVLTTDWYKKQDKS
                     QLIHRLIDLLRFTTVKYTRSKVRKGLPKEYSYIIEELLYIDDRINGKKDYVKKIIEQL
                     VLMKEEEKFLTKLAQTIQKSVIDHLHIVGDIFDRGTQAAKVMDRIMDFSSVDIQWGNH
                     DILWIGAYCGSEACLLTLLRIAARYNYLFELEKEYGLNLRPLFSFAHQTYQKNPVFTP
                     KNRKDLSEREQEELEKIHQALSILQFKSEHQLLNRRPEFKMDDRKLLEKINYEESTIV
                     LEGQLYELKGTCFQTIQPDDPKKFLEEEQKVIDSLMYSFQHSLRMQKHMTFLIEKGGM
                     YLTNNQHVLFHGCIPVDEKGQLLAFELDQSYRGKELLGFFEKQITESAKDLTANEDLA
                     TDLIWYAWSGPFSPLFGKSKMATFERYFLTEPHTHVEKSNPYYHLRDEEWFSEKILAE
                     FDAKEEGSAIINGHTPVKVKKGESPIKANGKAFVIDGGLSKAYQKTTGIAGYSLLCNS
                     YGFQIVTHYPFLPIEQQFAKKSDQTNVKKVIEQQLPRKLNRNTTKGMELQQQITQLQR
                     LLDYRKDD"
     gene            complement(25064..25783)
                     /locus_tag="IWO89_000511"
     CDS             complement(25064..25783)
                     /locus_tag="IWO89_000511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyl-gamma-aminobutyrate hydrolase
                     family protein"
                     /protein_id="HAQ0455293.1"
                     /translation="MKPIIGIAGNQLIRATDTFQGNQVSYTPQGFVDAVLDAQGLPLI
                     LPVMSPDSAPQLIEKIDKLILAGGQDVSPQLYMEDPHPKLTETNIQRDQFEQALILEA
                     LKQRKPIFAVCRGLQLLNVVLEGTLYQDLSLYPKWSVKHEQQPTAPQFATHEVKIVSD
                     SLLSDLLPDSYFVNSYHHQAVKDLSPLLKAIAFSNDGLIEAVQSKDNMHKILGVQWHP
                     ELSHRVDSSEQRLFDFFVNEF"
     gene            26120..26452
                     /locus_tag="IWO89_000512"
     CDS             26120..26452
                     /locus_tag="IWO89_000512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0455294.1"
                     /translation="MKQVLEALEQIRKAEEENEKRARTLRQQLKTYAEEKEQELEIIQ
                     TNSRQQLKQLVEEKEQFEKQELEKEKMKLKAEADKVQATLKDQYQKHQSQAIGAIIER
                     VKETYGRH"
     gene            26439..28442
                     /locus_tag="IWO89_000513"
     CDS             26439..28442
                     /locus_tag="IWO89_000513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit I"
                     /protein_id="HAQ0455295.1"
                     /translation="MAVTKMEKVTLISDKRNQERILQAVQGIQNVEIRDLYQNRTNNQ
                     WVEKYFPDTTIIDKEAKLSALGNRLKDIQEAIQFIQHHGDSRQKKLHLKRQELSLQDL
                     EANYSEENFMNKLEEVLDLKEKWEQLTEKRDRLEEEEEWLLHWQQLDIAPSMYASKTT
                     IFEMGTVSAANFESFKEELSKLDDVYMEEIDYTPKQVYLSYVALKKSVAAIEEIAGKY
                     GFNKETYPYEQNPKEKLQETKQQLSIVHEQQKKITTALGQCSGYIQEFEWVEEVTLAV
                     AEREKVKERFIHATYLIVLQGWVDAEEKQNLMHVLNETVSEEDIYVSFETPNSEEIQQ
                     EVPTKLKNHPLIEPFELLTEMYSLPKYEEVDPTPWMTPFYLVFFGMMVADVGYGLLML
                     IGTILAQKLFVLPRGMKRFVKFFEILSVPSIIWGLIYSSFFGQALPKELFGIRLPFPI
                     LSTTDDVNTILILSVIFGLIQILVGLFVAAKENIRRKDYLSAVSDGFAWQGILIGIVI
                     ALIGVMLIKNTAFVYLGAGLSIIAALCILIVPIIQSPSKVKGAAKGAYNLYGLTGYIG
                     DLVSYTRLMALGISGGSIGAAFNMLVAFMPPAARFSVGILLIIALHALNMFLTLLSAY
                     VHGARLQYVEFFGKFYTGGGRAFDPLKTAEKYVNINHKKKNNK"
     gene            28461..28931
                     /locus_tag="IWO89_000514"
     CDS             28461..28931
                     /locus_tag="IWO89_000514"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit K"
                     /protein_id="HAQ0455296.1"
                     /translation="MMDYLISQNGGMVFAVLAMATATIFSGIGSAKGVGMTGEAAAAL
                     TTSQPEKFGQALILQLLPGTQGLYGFVIAFLIFINLGNDMSVVQGLNFLGASLPIAFT
                     GLFSGIAQGKVAAAGIQILAKKPEHATKGIIFAAMVETYAILGFVISFLLVLNA"
     gene            28952..29536
                     /locus_tag="IWO89_000515"
     CDS             28952..29536
                     /locus_tag="IWO89_000515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase V"
                     /protein_id="HAQ0455297.1"
                     /translation="MNAIEKIISQMNEAAEQERAALEQEERMKIDQNFEQKRTQIETE
                     HQKQKEKQIELLEKKYRQLRNRQQVEVRQENLNAKQEFLRRLFADAIAEMENWDESEQ
                     IQFIKNSLYSLPLTGKVTFIAGEKSAAYLSQTLLDEWNNELPFMMVLSDETVADQAGF
                     LINDQGVQYNFLFSSLVQDIQGTMSFEIANQLFE"
     gene            29551..30537
                     /locus_tag="IWO89_000516"
     CDS             29551..30537
                     /locus_tag="IWO89_000516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATPase subunit"
                     /protein_id="HAQ0455298.1"
                     /translation="MEYHELNPLIRGRELELLSNETFERMIQTNSVEALGEILKSTIY
                     SPYIYDGYEYDFEQNLLKEQSRLFRWLKESAPEPEIVWIYTMRYTFHNLKVLTKAEMT
                     NQNLDHLYIDDGFYSLETLKDAIHTQVSNELPESIMDSIREVRDYFEESTILQGIDVI
                     YDRQFLTEQRRLADKLGYQELLTEVIAFIDLTNITTMARGILQKRTYGFMTTVLSSSG
                     SIEKEVFLNFVDKDMEDYTNFLLETDYADFLRPVIREGNLDLVKLEQLKDDYLSSLYQ
                     EAQTQAFGPLPLLAFLNAKEVESKNLRLLVIGKRSHFEPETIRKRVRQVYDA"
     gene            30527..30838
                     /locus_tag="IWO89_000517"
     CDS             30527..30838
                     /locus_tag="IWO89_000517"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007209277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit F"
                     /protein_id="HAQ0455299.1"
                     /translation="MTHKIGVVGDKDSVLPFKLFGFDVRYGTTKQTVRQAIEEMAKNE
                     YGVIYVTEQCAALSPETIERYKEQMIPAIVLIPNHQGSLGIGLEEIQKNVEKAVGQNI
                     L"
     gene            30860..32641
                     /locus_tag="IWO89_000518"
     CDS             30860..32641
                     /locus_tag="IWO89_000518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit A"
                     /protein_id="HAQ0455300.1"
                     /translation="MQIGKIIKVSGPLVMAENMSDASIQDMCLVGDLGVIGEIIEMRG
                     DVASIQVYEETSGIGPGEPVRSTGEALSVELGPGIISQMFDGIQRPLDTFMEITQSNF
                     LGRGVQLPALDHEKKWWFEPTVEAGETVSAGDVIGIVEETKVIKHKIMVPNGIKGTIK
                     TIEKGSFTIDETVCVIETENGDKNLTMLQKWPVRRSRPIKEKLNPDAPMITGQRVIDT
                     FFPVTKGGAAAVPGPFGAGKTVVQHQIAKWADVDMVVYVGCGERGNEMTDVLNEFPEL
                     IDPNTGESLMERTVLIANTSNMPVAAREASIYTGITIAEYFRDMGYDVAIMADSTSRW
                     AEALREMSGRLEEMPGDEGYPAYLGSRLAEYYERSGRVIALGSEGREGSITAISAVSP
                     SGGDISEPVTQNTLRVVKVFWGLDSSLAQKRHFPSIDWIQSYSLYSTEVGKYMDHILQ
                     ADWASMVTEGMRILQEEQQLNEIVRLVGIDSLSDNDRLTLEVAKSIREDYLQQNAFDD
                     VDTFTSREKQFKMLKMILTFGEEGRRALSLGAYLSEIMDGTVSIRERIGRSKYIPETE
                     LEKLDQIIGEIKQTMQTIVSEGGMSND"
     gene            32634..34010
                     /locus_tag="IWO89_000519"
     CDS             32634..34010
                     /locus_tag="IWO89_000519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit B"
                     /protein_id="HAQ0455301.1"
                     /translation="MIKEYRTIGEVVGPLMAVEKVAGVKYEELIEVRMQNGEIRRGQV
                     LEVQEDKAMVQIFEGTSGINLRDSSVRFLGHPLELGVSEDMIGRVFDGLGRPKDNGPE
                     ILPEKHLDINGEVINPVARDYPDEFIQTGISAIDHLNTLVRGQKLPVFSGSGLPHKEL
                     AAQIARQATVLDSSDDFAVVFAAIGITFEEAEFFMEDFRQTGAIDRSVMFMNLANDPA
                     IERIATPRMALTAAEYLAYEKGMHVLVIMTDMTNYAEALREISAARREVPGRRGYPGY
                     LYTNLATLFERAGRIRGLKGSVTQIPILTMPEDDKTHPIPDLTGYITEGQIILTRELY
                     KSGIQPPIDVLPSLSRLKDKGTGPGKTREDHAATMNQLFAAYAQGKQAKELAVVLGES
                     ALSDVDKIYAKFAERFEKEYVNQGFYTNRSITETLDLGWELLSMLPRTELKRIKDDML
                     DKYLPEGK"
     gene            34017..34652
                     /locus_tag="IWO89_000520"
     CDS             34017..34652
                     /locus_tag="IWO89_000520"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit D"
                     /protein_id="HAQ0455302.1"
                     /translation="MARLNVNPTRMELTRLKKQLTTATRGHKLLKDKQDELMRQFILL
                     IRKNNQLRQEMEESMTEAMSDFVLANASITEPFIEELFVLPAENVDLSIVDKNIMSVK
                     VPVMNFEYDEQLTDTPLEYGYLNSSAELDRSIDRFTTLLPKLLELTEIEKTCQLMASE
                     IEKTRRRVNALEYMTIPQLEETIYYIRMKLEENERAEVTRLIKVKNMGTTD"
     gene            34682..36037
                     /locus_tag="IWO89_000521"
     CDS             34682..36037
                     /locus_tag="IWO89_000521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Trk family potassium uptake protein"
                     /protein_id="HAQ0455303.1"
                     /translation="MIKQSKQIKKRLPAVQLLALGFFLLILVGGGLLTLPFFSNSGHG
                     TNFIDALFTATSAVCVTGLTTLNTAEHWNSAGQFLIMLLIEIGGLGFMMVPILCFALI
                     KKKVSLSTRIILKEALNLEEVSGVMKLMLYILKLAFVIQLIGTVALSFVFVPEFGWLK
                     GIWYSLFHAISSFCNAGFDLLGDSLANHQQNVYLIMVVSALIISGGLGFIVWRDLLEY
                     HQKKKMTIHSKIALTVTGTLLLGGFVVFFFTERNGATLVEGNMFQRLANTFFMSVTPR
                     TAGYYSVDYLHMSHAGLILTMFLMYIGGTSGSTAGGLKTTTLGVLLIQMRSMLKGRTR
                     AEAFGRTIRQGAVLRALTLFFVTLTLCVVAIMILSVTETIPKTSGIEYIAFEVFSAFG
                     TVGLTMGLTPDLTAIGKLIIIALMYIGRVGVLTVVFSLMTKAQRTQSKYKYPEESVMI
                     G"
     gene            complement(36229..37623)
                     /locus_tag="IWO89_000522"
     CDS             complement(36229..37623)
                     /locus_tag="IWO89_000522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HAQ0455304.1"
                     /translation="MPINSYDNYPLTWRPDKQKLKRPYYRSLIQQLEQDILKNKLQKN
                     TQLPSQRELADYLDLNFTTVGKAYKYGIEKGLLYTNIGSGTFISPNVFESITISTNEV
                     TEHIIDFGLVSSFEECNKFVVPFIQSTSNNPNLVGLLNYQDPKGTNFQLTTAANWLKT
                     QGVIPSLESLSIVSGVQNGLAITLSALFSPGDRIATDRYTYANFIELAQLYHLEIVPI
                     DFDEQGMRPDILIQECRKKKIHGVFLMPSCNNPIGFQISHKRREQLAEVIEKEGLWVI
                     EDDIHSFLTTYYQHTIIPPFQNLLPNQTIYLAGMTKFICSGLRVAYLVVPVHVQSKIE
                     QAIFNINVKTSGLDVEVITQILHSNEANRILETKLSLTKRANQLFDSLFKVEKPTNRV
                     PYYRILPVDPEIDPKIIENDFLANGIRLYHSRRFTTRAQPDAFVRISLSSNNLEILAS
                     GLSIVKEKIKKYEL"
     gene            37715..38686
                     /locus_tag="IWO89_000523"
     CDS             37715..38686
                     /locus_tag="IWO89_000523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine cyclodeaminase family protein"
                     /protein_id="HAQ0455305.1"
                     /translation="MKIINFEEVVASFSFEEAIQVMKKCFFDYESGKISQNPRLVEIL
                     PDGKGKNMFAMMPAYLGENRYFGTKVITAFPDNHQYHLPSHLGEIFLFDAQNGLPKAL
                     INANAVTWIRTAAVSALATDYLANPHAETLALIGAGQQALSHLTAISTIRPITTVFVY
                     DILEERRNTFIAKAKKQYPKITFINAISVEDAVRPAEIICTLTSSKEAFLQKEWVRED
                     AHINAVGTFTPDTREISTSLMETSQIYVDDYSAALTESGDLLIAMAEGDLEESCIVGT
                     LGELVSEKKTFYKGESRPTIFDAVGLAIEDLCCAEYIYNKLEKGALK"
     gene            38683..39465
                     /locus_tag="IWO89_000524"
     CDS             38683..39465
                     /locus_tag="IWO89_000524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhzF family phenazine biosynthesis protein"
                     /protein_id="HAQ0455306.1"
                     /translation="MNLDYYVVDAFADEVFKGNPAAVYVLEEWLPEGTMQKIAIENNL
                     SETAFTVKKNQEFELRWFTPDREIDLCGHATLATAFVLFNYYKIPDETIKFSTQSGNL
                     FVTRIKDYYYMDFPSIMPKKVPILSEYEEAIGAKIKEAYLARDLFFVLEDEKTVAKLQ
                     PDFTAIKNFELGIGVIVTAESVQKDFVSRTFFPKLTINEDPVCGSAHSNLIPYWAERM
                     NKTKLSAYQMSSRGGDLECELKEDRVIIGGKAVLFSKGTSFI"
     gene            complement(39634..40005)
                     /locus_tag="IWO89_000525"
     CDS             complement(39634..40005)
                     /locus_tag="IWO89_000525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactoylglutathione lyase"
                     /protein_id="HAQ0455307.1"
                     /translation="MKMAHTCVRVKDLEASIEFYQKAFGFEESRRRDFPENKFTLVYL
                     TLPGDDYELELTYNYDHGAYDLGDGYGHIAIAADDLEKLHEQHKAAGLEITDLKGLPG
                     TAPSYYFVIDPDGYKIEVIRG"
     gene            40139..40582
                     /locus_tag="IWO89_000526"
     CDS             40139..40582
                     /locus_tag="IWO89_000526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HAQ0455308.1"
                     /translation="MDKPIFGEKKQFADYQTRYAAYIVIERDQQIAVIEAPNGAFFLP
                     GGEIEGEETKEEAIVRELIEEMGIVAEISSYLGEADEYFYSNYRATYYYNPGYFFVAD
                     SWKRIGEPTEKTNKIWWVSPQEALVKLKRGSHRWAVERWIEKNKN"
     gene            complement(40625..41434)
                     /gene="yidA"
                     /locus_tag="IWO89_000527"
     CDS             complement(40625..41434)
                     /gene="yidA"
                     /locus_tag="IWO89_000527"
                     /EC_number="3.1.3.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-phosphatase"
                     /protein_id="HAQ0455309.1"
                     /translation="MSIKLVAIDIDGTLLNEKREVTKEVKEAIAAAVAKGVSIVLCTG
                     RPLPGVQEQLNELNLFQDNDYVITYNGALVQQTKSGKIIARHGLTHEDFLEIEVMARR
                     VGSHLHSIDDKAIYTPNRDISAYTIHEASLVKMPLKYRTPEEMTPDMNIVKMMMIDEP
                     EILDAAIARLPQTFRDKYTTVKSAPYYFEVLNKEASKGAAVANLAQHLGIDQDEIMAI
                     GDNENDLSMIEYAGLGVAMGNAVSLVKEAANVITSSNDEHGVAEAIKKYVL"
     gene            complement(41453..42820)
                     /locus_tag="IWO89_000528"
     CDS             complement(41453..42820)
                     /locus_tag="IWO89_000528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HAQ0455310.1"
                     /translation="MTTPYKYQMLPMEKVFRDPVHNYIHVQHQVILDLINSKEVQRLR
                     RIKQLGTSSFTFHGAEHSRFTHSLGVYEISRRICDIFSRNFSKEKIGNGGWDDQERLV
                     TLCAALLHDVGHGPYSHTFEHIFHTNHEAITVEIITSPETEVYQILNRVEEGFPEKVA
                     SVIQKTYPNPQVVQMISSQIDADRMDYLLRDAYFTGTEYGTFDLTRILRVIRPYEGGI
                     AFSMSGMHAVEDYIVSRYQMYVQVYFHPVSRGMEVILEHLLHRAHELYQEDASSFELH
                     SQLLLPFLDGSFTLEEYLKLDDGVLGTYFTQWSESADPILNDLAKRFLNRKPLKSATF
                     SGNRDSKLVQELTLLVEKVGYNPVYYTAVNSSYDLPYDFYRPEQGRHRTQIEILRNDG
                     TLIELSQVSQLVAALAGQEQGDERFFFPKEMVDPSLRDHYDLFDETYQEFASHIRNGA
                     LIEIN"
     gene            complement(42804..43634)
                     /locus_tag="IWO89_000529"
     CDS             complement(42804..43634)
                     /locus_tag="IWO89_000529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase family protein"
                     /protein_id="HAQ0455311.1"
                     /translation="MNPAIRLDQHIYQKNDFSPFALTDILTEYSKEHQQIFLHFWQYD
                     CTVILGMKDVRTPFLSDGIQTLKDAGYAPVIRNSGGLGIVSDEGILNISLIFPQSDTK
                     KITIDDAYEQMLLLTKYAFPKAPIQAFEITHSYCPGTYDLSIHGKKFAGIAQRRIKNG
                     ISVMMYLSVNGNQEKRGQVMKRFYQSALKEDFGTNGYPAVDPATMATLEELLDQPLFV
                     KEVKEAFAKAFEKLYSASDLQDSTDWLKKEIQLEQWNNQIQRMEQRNNIKELHYDNPL
                     "
     gene            43797..44234
                     /locus_tag="IWO89_000530"
     CDS             43797..44234
                     /locus_tag="IWO89_000530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1934 domain-containing protein"
                     /protein_id="HAQ0455312.1"
                     /translation="MELSQGTPISIKLRTKVKQQGEVQDFYFDLKGQMVTIGDTLYIR
                     YKEIQEETGDEIPVTIKLVPDGHVQLIRAGEMRMRLKFGYKERLETSYRTPYGMIQIA
                     TFTKELHVSLKDRPTGGKVRIDYDLFMGPEKIGEYYLTLDFTA"
     gene            44310..44930
                     /locus_tag="IWO89_000531"
     CDS             44310..44930
                     /locus_tag="IWO89_000531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit delta"
                     /protein_id="HAQ0455313.1"
                     /translation="MEISVFEGANKSELSMIEVAHAILEQRGDVMDFSDLANQIQNYL
                     GKSDSEIRDSLAQFYTDLNIDGSFISLGDNRWGLRSWYAIDSIDEEVNHGMDEEDEDT
                     PRRRKRKKVNAFINDDEDAIDYNDDDPEDTDLTEEDDDDLFDDDDDEDEEIAAYNSDL
                     QEIGADDPTDDEEDLPGNIEEDLSIIDEDDDDEDYEEEEFSDSEKE"
     gene            45119..45625
                     /locus_tag="IWO89_000532"
     CDS             45119..45625
                     /locus_tag="IWO89_000532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HAQ0455314.1"
                     /translation="MIFTGYKKKRQIEKYRHLLNGLIETDYQKMFRITMNYVHDKEEA
                     LDVMQDSFHKALNYFEKEKEIEHFSAWFYRILIRTALDSWRRQKRTPVDLFEIQDIPQ
                     QLPLETSVAELHMILDKIDSPEKEILILYFFEGFRLKEIATILDMNENTVKTKMYRSL
                     NALRQILE"
     gene            45642..46541
                     /locus_tag="IWO89_000533"
     CDS             45642..46541
                     /locus_tag="IWO89_000533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3298 and DUF4163 domain-containing protein"
                     /protein_id="HAQ0455315.1"
                     /translation="MYSQKENLKKLKKTYKEQAIPKGLKEEIRQRFIIEEQSFIKAKR
                     RKRRLQGFGVSLAIVMITTSSLLFNSQVRSFAEDLPILGSVIELILGERFTDRSEKID
                     IQVPKINTQNEKENKTIHGLNQKYFREGQADFEKAEKEYGNFETDHYQVLGDYQKIVD
                     DNRFLVIERQITQTAADSHVEKRYDTIDKKNSVQLSLPLLFKDDTYLSVLTKEVKRQM
                     AEQVKEDPSKYYWTEQDIQEGTIEKPTLVTPTRSFYLNKEHQLVLTFSQYEIAPGYMG
                     TPEFVIPKSVTKTILASEDYLDH"
     gene            46628..47512
                     /locus_tag="IWO89_000534"
     CDS             46628..47512
                     /locus_tag="IWO89_000534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HAQ0455316.1"
                     /translation="MKRNYLVISIIALSVLLFFEITSVASLITEVKGQTHSSEHSSQS
                     FKQSEQSTIKSQSTETANVKEAIQESAEKVQSQSSQSTQDSTKTSRDPAVKSVAISFD
                     DGPGATTTPQLLRILKEKNVHATFFVLGENTAQHPEIVKQTAEAGHEIGNYTYDHQNL
                     ATLSAQSMTEEVTKADTEIKKVTGKTPTFVRPPYGSVTNVGATIIQRPIIEWSVDSED
                     WKTRNPDLILQKIQATVYDGAIILFHDIYPETIRAVPQVIDYLKEQGYRITTVGDLLG
                     HPTAVENYYGRNDHRPVQ"
     gene            complement(47704..47775)
                     /locus_tag="IWO89_000535"
     tRNA            complement(47704..47775)
                     /locus_tag="IWO89_000535"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(47740..47742),aa:Arg,seq:ccg)
     gene            47835..49169
                     /gene="rpoN"
                     /locus_tag="IWO89_000536"
     CDS             47835..49169
                     /gene="rpoN"
                     /locus_tag="IWO89_000536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase factor sigma-54"
                     /protein_id="HAQ0455317.1"
                     /translation="MKFEQNFSQKQQQTQKLAMTQQLQQSIQVLQYNSEELLAFVENQ
                     AMENPLVEVVEPEWQPDYIKASSSSYEGEETNYLNQIPDTKGSLFDSLIEQVHLNYRD
                     TFLRKIVLYLVEYIDLNGFLTISLEEAGKEIGATPIQMLDALTLIQQLEPAGVGARSL
                     QECLMLQTERDDYAPELAYIVLEECFEELVERKWKEIAQRFSVDLHAVQQIFDYLQTL
                     SPSPGRIFDRSSELYIIPDVRVLVDDDKNVQVISNRKNQPNIRFQESYFKQMSQQADK
                     ETENYLKERKQEFEWLKKTILQRGDTILRVAQVIVSRQKAFFIDKERPIKPLTLKEVA
                     TEIDVHESTVSRAVNGKYLETDFGVFELKKFFTTRIPTNSTEQTEDLSADTAKKKLQE
                     LVDQEDKNKPLSDQKLVELLKKDEIAISRRTVAKYRDLLGIPSSSKRKRYDN"
     gene            49372..50409
                     /locus_tag="IWO89_000537"
     CDS             49372..50409
                     /locus_tag="IWO89_000537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SorC family transcriptional regulator"
                     /protein_id="HAQ0455318.1"
                     /translation="MLDEIKMIESVAPDIIEVLQERYKILRNIYWMQPIGRRSLSESM
                     GITERVLRTETDLLKNLNLIDTSKSGMTLTTKGEEVYQSLENFMDQLLGTHQTEQQLA
                     EYFGNQRCIVVSGNSEEQTKVADAFGEALSEALDRLLPEGENIIAVMGGTTMAVVAEQ
                     LSNLENKKRHNLFVPARGGIGEAITVQANSVSARMAAKANGNHRALYVPEQLSLATYN
                     SLLNEPSIQEVLNLISEANCVIHSIGRALHMAARRKMTEKELVMLKQANAVAESFGYF
                     FNEKGEVVYKVPRIGIELQDLEKVPIIMAIAGGKSKAKAIRAYMKNAPKQTWLITDEA
                     AANEILKGVTL"
     gene            50450..51451
                     /gene="gap"
                     /locus_tag="IWO89_000538"
     CDS             50450..51451
                     /gene="gap"
                     /locus_tag="IWO89_000538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010753170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HAQ0455319.1"
                     /translation="MTVKVGINGFGRIGRLAFRRIQDVDGIEVVAINDLTDAKMLAHL
                     LKYDTTQGRFNGTVEVHEGSFNVNGKEVKVLANRNPEELPWGELGVDIVLECTGFFTS
                     KSAAEKHLTAGAKRVVISAPGGNDVPTIVYNTNHETLTGKETVISGASCTTNCLAPMA
                     KTLNDKFGVVEGLMTTIHAYTGDQMTLDGPHPKGDFRRARAAAANIVPNSTGAAKAIG
                     LVIPELNGKLDGAAQRVPVPTGSLTELVTVLEKEVTVDEINAAMKEASNESYGYNTDE
                     IVSSDIVGMTYGSLFDATQTKVMTVGDKQLVKTVAWYDNEMSYTAQLVRTLEYFANL"
     gene            51561..52751
                     /locus_tag="IWO89_000539"
     CDS             51561..52751
                     /locus_tag="IWO89_000539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005228679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HAQ0455320.1"
                     /translation="MAKKTVKDIDLKDKKVLVRVDFNVPLKDGVITDDTRIKAALPTI
                     NYVLEQGGKAILFSHLGRVKTEEDKEGKSLAPVAKRLGELLGKEVTFVPETRGEQLEE
                     AIRNMNDGDVVVFENTRFEDIDGKKESKNDTELGKYWASLGDVFVNDAFGTAHRAHAS
                     NVGIASTGIPTVAGFLMEKEIKFIGEAVEEPKRPMIAILGGAKVSDKIGVIENLIPKA
                     DKILIGGGMTYTFYEAKGIKIGNSLVEADKVELAKELIEKAGDKLVLPIDNVCAPEFS
                     NDVETQVIEGDIPDGLMALDIGPASVKLFADTLQGAKTVVWNGPMGVFEMSNFAKGTI
                     GVCEAIANLEGATTIIGGGDSAAAAEQLGFADKFTHISTGGGASLELLEGKELPGLAA
                     INDK"
     gene            52855..53610
                     /locus_tag="IWO89_000540"
     CDS             52855..53610
                     /locus_tag="IWO89_000540"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HAQ0455321.1"
                     /translation="MRKPIIAGNWKMNKTASEAKEFAEAVKNKIPSNDKVDSVIGSPA
                     LFLQELMTSAEGTELKISAQNCYWENSGAFTGETSPAALADLGVHYVIIGHSERREYF
                     HETDEDINKKAKAIFANGMTPILCCGESLETYEAGKTAEWIEGQITAGLADLSAEQVS
                     NMVIAYEPIWAIGTGKSADAQIADEICGVVRQTVEKLYGKEVSEAVRIQYGGSVKPEN
                     IAEYMAKENVDGALVGGASLQADSFLSLLDAVK"
     gene            53726..55024
                     /gene="eno"
                     /locus_tag="IWO89_000541"
     CDS             53726..55024
                     /gene="eno"
                     /locus_tag="IWO89_000541"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013773781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HAQ0455322.1"
                     /translation="MSIITDVYAREILDSRGNPTIEVEVYTESGAFGRGMVPSGASTG
                     EYEAVELRDGDKARYGGKGVTKAVDNVNNIIAEAIIGYDVRDQMAIDKAMIALDGTPN
                     KGKLGANAILGVSIAVARAAADYLEVPLYHYLGGFNTKVLPTPMMNIINGGSHADNSI
                     DFQEFMIMPVGAPTFKEALRMGAEVFHALASILKARGLATSVGDEGGFAPNLGSNEEG
                     FEVIIEAIEKAGYVPGKDVVLAMDAASSEFYDKEKGVYVLADSGEGEKTTDEMIKFYE
                     ELVSKYPIISIEDGLDENDWDGFKKLTDVLGDKVQLVGDDLFVTNTQKLSEGIEKGIA
                     NSILIKVNQIGTLTETFEAIEMAKEAGYTAVVSHRSGETEDSTISDIAVATNAGQIKT
                     GSLSRTDRIAKYNQLLRIEDQLGEVAEYKGLKSFYNLKNK"
     gene            55423..56562
                     /locus_tag="IWO89_000542"
     CDS             55423..56562
                     /locus_tag="IWO89_000542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArgE/DapE family deacylase"
                     /protein_id="HAQ0455323.1"
                     /translation="MKKEEKIAILQEIIRIKSVNGNEGEVAAYLNKLLAKHDITGEIV
                     SYRDGRDNLIARYQKGQSGKVLGLSGHMDVVAAGDESSWTYAPFAAEIHGNRLYGRGA
                     TDMKSGLAAMVIAMIELKESGKPFNGTVKLLATVGEEVGELGGEQLTKAGYVDDLDAL
                     IIGEPTNYSLMYTHMGSINYTVTSHGKEAHSSMPDQGYNAINHLNEFITKANAEMNHL
                     AETIENPVLGKTIHNVTLISGGNQVNSIPSHAQLQGNIRSIPEYPNDKIIALLQSIVN
                     ELNQETDYHLELMIDYNKIPVKADPDSPLIHCIQQQFSQPLPLVGAAATTDAAEFTKA
                     NHSFDFVVFGPGVVTLPHQVDEYVEIDNYLDMIEKYQGIILSYLA"
BASE COUNT        18779 a         9857 c        11984 g        15954 t
ORIGIN      
        1 atcgagaggt tgtgaaaaag tcgcttagtt tcgagtagta agaaaaattt tgtgaaaatg
       61 gctttccaca tttttgcata aatttgactt aataccgaga aaatggcttt tgaacaccgt
      121 ttattcaaaa aaagggactt tgacactact tttgtcaaag tcccatcggt tttttatcta
      181 tttttattac aatcatttct tccagagtag tgacatctgg aatggctgaa atgtaaacag
      241 attatttgcc tgtatcaatg tcgaagagta atataaaaag tgtaaacgct atcatattgt
      301 tcatcattca aattttatag agagaaggca ttgagatgga aaaaacatat caagcaggta
      361 caaatgaagg aatagtagat tttatcaata tggaagattt agaaatcgcg gcttcacaag
      421 tgattccagc tggtggttat ggttacatca gcagtggtgc tggcgactta tttacttatc
      481 aagaaaatga acgggcattc aatcatcgtt tgattattcc tcatgtatta agagatgtag
      541 aattacccga tacaactaca cattttgacg aagaaatgct gactgcacca atcatcatgg
      601 caccagttgc tgctcatgga ttagctcatg tgaaggcgga gaaagcttct gcaaaaggtg
      661 tagcagattt tgggacgatc tatacagcaa gttcttacgc ttcttgtact ttagaagaga
      721 taagagaagc aggcggggag aaagcaccgc aatggtttca attttatatg agcaaagata
      781 atggaatcaa tttggatatt cttgaagtgg ccaagcgtaa tggagcaaaa gcaatcgtat
      841 tgacagcgga tgcaacagtt ggcggaaacc gtgaaacaga ccgcagaaac gggtttacct
      901 tcccgctgcc aatgccgatc gttcaggctt atcaatcagg agtaggccaa acgatggatg
      961 ctgtatataa atcttccaaa caaaaactta gtccaaaaga tgtagaattt atcgctgctc
     1021 attctgattt gccagtctac gtgaaaggtg ttcaatcaga agaagatgtc tatcgatcat
     1081 tagaatctgg tgctggtgga atttgggtat ctaaccatgg tggccgtcaa ttagatgggg
     1141 ggcctgctgc gtttgattcg ttgcaatatg tggcagaagc agtagataaa cgtgtaccaa
     1201 tcgtgtttga cagcggtgta cgacgtggac aacatgtttt caaagcaatc gcatcaggtg
     1261 ccgatcttgt tgctattggc agacccgtta tttatggatt gtctttagga ggaagtacag
     1321 gagtccatca agtgtttgat ttctttaaaa cagaattgga aatggtcatg caattagctg
     1381 gaacacaaac agtagaagat atcaaaaaaa taaaattgcg tgagaatcgt tttatttaag
     1441 aaataaatga cgaaaaaaat aggagtgcgg cagatgatgc cgtactccta tttttatagt
     1501 tcaaggatgt cactaataga ataatttgaa acgaaaaaat aaaattgaaa tattttttca
     1561 tacttaataa aaaaggaaga atattcatgt aaacgatttt attgtttttt tctcttttat
     1621 acaaaaaggg tcataatttc aagtaagcgg atgaattcat gaggattttt tgcatactgt
     1681 ctctactata cttaggtcac taagaaaaag gagtgattta ttatgaaaaa gaacatgatt
     1741 gggacattat tactttcaac acttcttcta ggcggcttgg caacaccagc ttttgctgag
     1801 ggtcaagcga cttcaaaagg agatattacc tttactgaac caacaaatac cgttgagcct
     1861 cttaacccta ctgatccttc aaaaccagta gagccagcag atccggaaaa tccagcaact
     1921 ggccagacag gttctttaac attggatgta gttcctgaat tgccatttgg tacacacgag
     1981 attgaaagcg gaacaaaaac gtatcaagta gacgcttcaa aaaacgatac accttatctc
     2041 caagtatcag atcgtcgagg tgtaggtgca gatggccaag cacaagggtg gaacgtgaca
     2101 gtttcggttt cagattttgt aaacggttca caagttttgc aaggcgctga attagatttt
     2161 ggaacatcca ctgttaaaag tacgtcagat aacgaatcca ctggaccaac tagtcaaact
     2221 gtaactgggt tgagtaaagc aagtgcagcg acaccaatat ttacagcagc aaaagatcaa
     2281 ggtctgggaa catggctttc tgtttatgat ccagcaaata tcacactgaa agtgccgaaa
     2341 gcagcagctg gaacattcac ggctgatttg acttggaacc tcgtggctgg cccagtagca
     2401 tagagtatga aataaattca acaaactgcg gtgaaaatac tataggtgaa ggatttttta
     2461 ccgcagttgt tgttgaaagg atgtggtgga caatgaatca aagtaaacga ctgtttgttt
     2521 cgtttttttc tattttaagt ctatttttca tttttccgtc aatcagccaa gcaaaggatt
     2581 cagcaggaga ctttggaatc aaaccggtat ttccagagaa ccaaatagat aaagcaatcg
     2641 gttattttga tttattagtg gcaccggaac aaaatcagac actagaagta attatcagca
     2701 attcttcaga tgaagaacga acatttgagg tctctgtcaa tccggcggta acgagtgatg
     2761 gtggaacgat cgattatagt caaaaaaacc caacgttaga tgagacattg ccgtttgatg
     2821 tccgtgacgt tttgctcatt gcgaaaaagg aaatcaatgt atcagcacat gcagaaacaa
     2881 ctatacctat agaagtgaaa atcccagcaa aaagctttaa gggacgtgta ttagcgggta
     2941 ttcatgtttc acctaaagaa gaagctgaaa cagaaaatgc tacagaagga gcacaaatca
     3001 aaaatcgtat tgcttataat ctcgcagtcg ttttacaaga aagtcaagag acgatagaac
     3061 cggatctgaa acttttatca ggagatttgg acgaggtcaa cgcaaagcca actgttcagc
     3121 ttcgtttcca aaatcctcag cctaggatta tcagcaattt gatatttacg agcaagatct
     3181 tttatgaaaa tcagctttat attgaaaata cgtctaatga atttttagtt gcaccaaatt
     3241 cgaattttca cttgaatctt gatttagctg gtgataaagc aaaagccggc gattaccgcg
     3301 cagaaatcat agcaaaatca ggcgacagta atgaatggcg atttacacaa aactttacga
     3361 tcaaaaaaga aaaagcacaa aaagtaaatg aaaattccgt ttttgcagta caagaacaat
     3421 ctttcccatg gctttatgca ggattggttg ctgcagttct agtgttatgg ttgattttgc
     3481 tgtttttgtg ggtccacaag aaaaagaaaa aaggagaaac aacatgatga ggtgatttcg
     3541 ttgaaaacat cgatcttatt gggagggatt attgcaagtt tgctgttctt cagctggcaa
     3601 gagccagtcc aagcagatac caaaagttca ggtacgatta cattttatga tctaagtcca
     3661 gttccgcatc caagtggttc agtcaatggc gcatcaaaac agcagacaca gctaccgatt
     3721 tataaagaat ctaatcaaca tggcgtattc ccggagacga atgaagaaaa gatccgtttt
     3781 ctttatttga tcattggtat agaattatgg cttcttgcgt ggttgttgtt atggagaaga
     3841 aaggaaaagg aggatgaaca tgttgctaca aaataaattt tcattttttc gctccttctt
     3901 ttttacttcg ctttttgttt taagtatatt tatcttttca ccttcggcag aagctgttgg
     3961 agcaacgcct cctgccattt cttatgatcc atacacgtat gaaaatatcg tacctccaaa
     4021 tggtggacgt agtgcagtca atggcgggaa gcttgtccaa ctaactgggg ctaaccaatt
     4081 gcctaaattg actgctactt atttgaacga tggttcaaat gatccttgga aaggtggcac
     4141 ggtccgtttt cattgggaaa gccatgtgaa actaacaaat gtacttgatt tggtgaatat
     4201 cactgcacaa ggatttgcat ttgtggcgat catcaatttg ccggaacatg tatcaagcag
     4261 tgatgtatta aaagcaattg actggaacaa cgcctacttg tatattgccg gtgcccccat
     4321 caaggttact agcggtagtt tattatcagc aggaagccat accttgcgtt ttgggatggg
     4381 gtcgtgggat acaaatactc tattggcaga tattattgct attattacgc gaggcgaatt
     4441 caacgtggac gatatcccgc ttcaatttga tgttacagct aatgtcgcga agatgacaga
     4501 aaacggagat aaatatgata cgagtccaaa caaaattttg accaagggta aattacaacc
     4561 ttctatgtct aaaaaggccg ttttttctgt tgatttttat gattcgaata atattatcca
     4621 agatcgtatg ttcaattcga ttttagctcc agctgcaggt agattttatc ctaaattaac
     4681 tggcggaaac ttgaatccag gagcatatgc attttctgca agtgcagaga taaatacgtg
     4741 gaatagttac atctcaacaa gtgattcaac aggaaaatat aatacatttg ctgataccac
     4801 agaatacgtg gatggaaata accagaacct tccaggctct accaatagtc ggacattgac
     4861 gatggggctg actgaaggac gttttgatca gttgccattt agtcgctttg atcgcgtagt
     4921 caactatttt acaggaaaaa acgttacaca aggagcaata ctgactcata ctccagcaag
     4981 cacaagtgga ttgaatcaga cagccaatat cgtgtacagt gggaaagatg cgcaaggcgg
     5041 attgctttct cctgttgctt taaaggtatt ggagcagtac aagctagacg gaacggtttt
     5101 accaacaaac ttaacagccg atagtcagtg gggaaagctc ctttcttctg catcgtacca
     5161 agtcacggcc aattggtcag caactgaact aaaaaagggt tcgatccatt acaggcttta
     5221 ttcgaaagga acccaaaaat tagcgacagg atggacagat caacttttcc aaacgatcca
     5281 atcaaataac gggggtgtaa atacagctac ttctacacta cctgcattgg cttcagggca
     5341 atattatttt gattatcgct tggtggacga tgtacttact aacatgtatc cgtcttttgc
     5401 gtacaaatgg cagtctgaac aagctggaat caccactctt cctcaaataa cagtggctaa
     5461 ttttccatct gtgagtgcag atagtcagct aaaaaacctt agcagacaac ctgagacagg
     5521 agatcccctg tctgctctat caggagatac gattcaagaa aatgtgacat tcacgttaac
     5581 aaaatctggg gatgcgataa ccgacaaaaa aatcacgatc tcacttcctg aaaatactac
     5641 ttatatcaat ggttccttaa aattaaatgg tacaactatc gcagataccg gcattcaaca
     5701 aggagtttct gtcccagcag atctattgag caaaatagga gataccattc atcttactta
     5761 caactatcaa ttaaatactg tagatacttc tgtacaatcc gtcagcatac tgacaaaggc
     5821 agctgtactt tccagcaata ttacattagc agatggtgct aagcttccta acccagttgt
     5881 ccaaacatct gctaaaacaa ttcttgtccc caaacaagaa ttaacattag ttaatgtacc
     5941 agatgatttt acatttggta acgatctccc caaaccgttg aagacaagtt attatgaagc
     6001 aaaaggcgat ttttcttttg atgtgcggga tactcggctt ccaagcacgt ctccttggca
     6061 actgacaggg acattgactt ctttatttaa gaatgatcaa ggtcaagaat taagcggaac
     6121 aaaattatac ttcaatcatt ctggatcaaa acaattgatt cagcaaggtc aaaacacgtt
     6181 gatttatgag agtgatggaa cagctaaagg agaggttctc gttgactttc ccgataccga
     6241 tggtttactc ttagaggtaa atagcagtac gaatgctcaa cctggcgcaa cgtatcaagg
     6301 catggtcaca tgggaactga cagctgggcc aaccagctaa gtgattagaa tttgtatgga
     6361 tctgtgataa ctgtcacaga tcctcttttt caaggatgag aaggaaactt ctgctgttaa
     6421 aatcagaaat gcttggagga gtggacttgg aacttttcag tttgcttgaa aaaaatgaac
     6481 aacggcaaat caaacttttc caacttttat tagaggaaaa aggagagatt tccgttaata
     6541 acgtacttac ccgcttatca gttgatcggg ctaccttgaa agaagatctg ttttatttac
     6601 aagctaactt ggaaaatatc aaacaattgg aaattgttat ccaagaaggg aatattttat
     6661 taatcagaca tggaaatata gcagcagtcg atgtttatta tcattgctac ttgcgctatt
     6721 ctacaaaata tcagatactt ctttatctgt tggaaaatgg ctcatttgat cggcagaaac
     6781 ttgtcatgga acttaatatc agttcggcta ctttgtctcg cagagtgaag gaattaaatg
     6841 attctttaaa tgagtttcat ctgaaaataa aaagcgggcg gctgaccggt ccagaaagtc
     6901 aaatttgtta tttttatttc caattatttt ggtttggacg tccttatctt gaaaacaaaa
     6961 aagaatacag tgatttatca ggaaacactt ttttagatct gctgcaaaaa gagtttagtt
     7021 ttactattac agaagaagga cgattgaaat ttgaaatatg gctttccatt atgaagaagc
     7081 gaatgcgctg tacgaaaaaa tccaaaccag attatagcaa tcttcctgtt ggattgtttc
     7141 aaacgacaca atttttattg gacctccata catttttgac ccgttacttc tttcaatatg
     7201 cctttccttg gaatcaaaat gaaacgttgg ttttttatct tttcatgatc agtaacttta
     7261 cattagatac acataactcg catgtccagg agtttttgat aagcgaaaaa tacaaagacg
     7321 acgtagtagc tcaattgaat cgaacgttta aacaatcgat agaagaatat ttctcttttt
     7381 atgtttttcc agaagagatt tcgcaaaaga ttgcgttgac agtgatgcaa aatcacgctc
     7441 attttgccta ttttagagga tggatgaata tttttggcca agaaggatta aaacagcggt
     7501 taactgaagt agaagataaa caattcctgg ttttatgtga aagattggtc agacaaacat
     7561 ttgctattgt tgattttact gaagtagacg aggaaaatgc ccgctttgaa ttatttgctc
     7621 gctatgcatc tattttgcgg acgctgatca agcagttaga attacgtctg caaattgctt
     7681 atgatcttcc ttatgaaagg ctaatggcgg atatcgtgaa agaaatcatc caggaacaat
     7741 tgctgaatac gatcaaatat gatttagtcg aatacgagaa agaaaagcag tatgatatca
     7801 ttttgactag ccaattgaaa gagtacccgt cacagaagtc cgctaaagtt tttgtattca
     7861 ctagcaacga atataattat gatttcccct atttgaacca atttttaaaa gaatgttatt
     7921 tagaaaaatt gaatttaagg atgaataaaa catgaaaatc gtgatcattg gtgcttccca
     7981 tgcaggcatc acagctgccc taaatctaag aaaacttcaa ccgaaagcag aagttttact
     8041 gatcgatgaa gatcataaag atggactagg ctatgtttca aatggaatca acctttattt
     8101 aaaaggaaag atacgtagtt tagctgaagt tgcccataac atgcggactc ttcaagaaag
     8161 tggggcaaaa ctgatcaccg aatggagagt aactgaactt gatccagaca aacatcagct
     8221 catcctcgca tccaaagaag gaaaaaacga gaccataacc tatgataaat taatcgtagc
     8281 aacaggttcc tctcctgtca ccttgtataa acaaattgaa gctgaaaatg tctacaccta
     8341 taaaaatcta gtgcaatcta agcaagtact tgccgcttta aaagaagcaa aagaagtagt
     8401 gatcttcggt gctggttata ttggattgga attagcagat gctttgagaa ataaaggata
     8461 cattattcat ctagttgatt atatgccgaa tgttttatca agatatttcg ataaggatat
     8521 gatcaacagt tttcaaaatc agctgcaaac taagcaaatc aatttttatc caaatgaatt
     8581 cttgatcgat tggaaaaaat cagaagagaa agtagtatcc gtacaacttc tatctcaagc
     8641 aataaaggca gatatggtca ttttttctgc ccaaactcgt cctaatacga cattgttaaa
     8701 agaaaaagtg gctttgtatg aggatgagac tgtgatagtc aatgaatatc tgcagacaag
     8761 tgatccggac atctatgcga ttggagatat cgtcccagtc agttttgaca aaaataaacg
     8821 gcatctcttt ttacctttag ttactcgagc tgtacatatg gcaagagcag tagcgctaac
     8881 tttgagtggt cagccaaccg cttacgacct cagacaaaag ataacagcag cagtcattat
     8941 agactacttt ttaggaactg tagggctgac agaagatgaa gcgccattct tggaacaaaa
     9001 taccggttcg tgttcaggag aatttgatct atttccacaa tacgatgaag aaaacaaaac
     9061 agtaaatgca aagctgatct accatccgga tacattagaa ataattggcg gacagctgat
     9121 aagccaagaa ttcttattat cagatttgaa cctgcttgcg gatatcgtga aacacaaaac
     9181 tacgattcca cagctagcag ttgaggattt tggatttctg tctgaacaca cctctcgttt
     9241 tcactacctc aatgagttag cttttaaagt actagctaag agagcaaata gcaatagagt
     9301 agataaaaga ctgtgagaaa tatcacggtc ttttattgtg tggggatata ataaatatta
     9361 atcaatcgag aataaaaaat tcatataatg aaatagcgga agaatttttt gagcataata
     9421 ttcgccaaaa atttatcaag tgataaaatt gttgtataaa atgattattt caggaggagg
     9481 agttaaatgt ttttagcatt aaatgaaatt atgcattcaa agttacgtta tgcgttagtt
     9541 gcaggggtga tgtttttgat cgcctacctg gtattttttt tgactgggtt agcttatggc
     9601 ttagctcaag ataatcgaac agcagtcgac aagtgggagg cagattcgat tgtgctttcg
     9661 aaagatgcaa attcaaatct agggatgtcc atgattacta agaagatagc agaagaagtc
     9721 gaaggtggga aagtggcgta tcttgcccag acacctggag tcgtgactag taaagacagt
     9781 acagaagaag gaaaaatcaa tgttagcttc ttcggtatcg ataaaaatca attcatcatg
     9841 cccaatcttg ttgaaggaaa agcttttgat aacgatgatg aggcagtagg cgatattagt
     9901 ttgaaagaag aatatggtct agccgtagga gatacagtta agttatctgg tagtgacaag
     9961 acgtttaaac taactggctt tacggatcat gccaaattca atgtctcacc agttctctat
    10021 acaaccatca atgcttacca agagatccgc ttcgaaaaag aagatacaag tgaaaatgca
    10081 cggattaacg caatagttgt cagaggtaaa atcagtaatt tacctgaaga tttagaacag
    10141 atcaaaattt ctaaattcat caatgaatta cctggatata atgcgcaagt attgactttt
    10201 ggatttatga tcggattttt gatcgttatt gcggctattg tgatcgggat tttcatttat
    10261 gtactgacga tgcaaaagat taatatcttt ggtgtaatga aagcacaagg gatcacagga
    10321 ggatttattg cgcgttctgt agtggcacaa acattcattt tatcatttgt tggtattctt
    10381 cttggtctat taggaacagt gggaacatcc cttgttctac ctgatgcggt tccattccaa
    10441 agcaattggc tgttttttgg cgtcattagc ttgttgatgc tggtagtagc tgtgttaggc
    10501 gcactatttt ctgttcggac aatcgtaaaa atcgatccgt tgaaggcaat tggataagaa
    10561 ggaggaagag catgaaagca atagaattta aagaagtaga aaaaagtttc caagatggcg
    10621 accaagtagt taaagcattg aaagaaacaa ccatgagtat cgataaaggg gaatttgttg
    10681 caatcatcgg tccttctggt tcaggtaaaa gtactttttt gactttggca ggcggattgc
    10741 agacgcctag cagtggctct gtatttatca atgatcaaga attcagtaat caaaaagaaa
    10801 gtgaacgcgt taaactacgt tttaaagaaa ttggctttat tttgcaagct tccaatctgg
    10861 ttccttttct gacagtaaaa aagcagttat tattagtaga taagatcaaa catgaacatc
    10921 gtcaaaaaga agcaaaagaa ttatttgagc agcttggcgt ggataagttg atcaacaagt
    10981 atccagaaga actatctggt ggagaacggc aaagggtcgc aatcgctcgt gcattgtaca
    11041 atgatccgtc cattattcta gcagatgaac caactgctag tttggattca gaaaaggcac
    11101 gagaagtagt cgatatcttg gcaaaagaat caaaagacaa aaataaagca acgatcatgg
    11161 ttactcacga tacacggctg attgacaaat gcgatagaat atttatgatg gaagatggtg
    11221 tattgaaaga aaaagaataa ccaaaaagca ggtggaaaaa tggtccatcg atacaacaaa
    11281 gatacacagc aaaaaatttt gaaagaagcg actgacttgt ttatgtccaa gggatatctc
    11341 ggcacttcaa caagagagat cgcacaaaaa gttgggatca cccaacccaa tctctatcat
    11401 tatttcggtg ataaagaaaa actatatacg gcagttttag aaaaccactt aaaagatgtt
    11461 ggaaaagctt tgagagaaat cgttcaaaca agcgaaacgg gatttcaagt aaccttgaca
    11521 aaaatggcgc agtttttgat tgagacacat ctggtcgatt tgtttctgat gcttcacgat
    11581 cttgaaagca atctttcaaa ggaaacaaga gatcatcttt tctttctatg gaagaaaaat
    11641 tatcgtgaac cttttgaaga aatcttttca gcaaaccaat ctgtgctaag aaatgggatt
    11701 tcaaaagaaa tcgcagcaag gcattttttc ttagtgttag caccttacat cactcggtcg
    11761 aaaagacctg tggaagaacc gctaaatgtg gcgcaattga tcgatttata tttgaatggt
    11821 gtattaggag aagcgtaaag ctgttatata gggagaaaaa tagttaggtg aagaagcaag
    11881 atatgacgaa gatcgttata tcttgttttt tttatatagg tagtttttca taaatacaaa
    11941 tttgttattc tctcatgatt atcagggtat atttttattt attatgtcgg tgttccagga
    12001 agtgttttaa tttaataacg aagaaattaa ttgtttgtag aatgaaggaa actatttatt
    12061 gggtagatga atcaccacga atagtcaaaa ggagatttca ggatatgcca aaaatgaaaa
    12121 aaatcatttt agcagtaatg ttattttcta cggttatatc ttttacagcc ccttttttgc
    12181 gtacttaagc tgattttaag ttttgaccct tagtctggat ctacgaggtg agagtcaaga
    12241 atgaaaaaaa taattggagc ttttgtattt gcaatcatta tcattattat ctgttttcaa
    12301 atagatgttt ttggtcaaaa aaagatagtt ggacaaacgg tcagcagtac gaacagtacg
    12361 gttacaaaga caaaggaatc atttgatgat acagaacaag agacattaat caaaatcgga
    12421 aaaaatgacc gtacactata tacagatgac tcgctaacta ccccacttgc aactataaac
    12481 ggcggagagc tggcggagtt tctttctgaa acaaaaaatg cttatcagat caagacaaat
    12541 gatggctata ctggatattt agccctcgaa gatggaacca aagtcacaaa gaatatacaa
    12601 acaaaaccgg aagagctatc tgatgcagtg attgtattag atcctgggca tggcggaaat
    12661 gatactgggg cattaagcaa tgatgaacaa acagaagaaa aagatataac attaagtaca
    12721 gccataaaag taaaaaaggc gctagaggat gctggagcaa cggtttattt gacccatact
    12781 acagatgaat tagtacagct tggagatata tgcgactaca gtgaagaaaa aaaggcagat
    12841 gtctttatca gtttacacgc agactctacg gaatatgcga atgaagcaac tggaatcacg
    12901 acatattatt actatggaca agaggaaaca ttagcacaga caattgctga ctccttcact
    12961 gatctgcctc tggattcaag aggaatctcc actgggaact atcaagttct tcgagaaaat
    13021 cttcaacctt ctattttaat cgagatgggt tatatgaata atgatagtga tttaaaggaa
    13081 ttagtaacag acagttatca gcagcagatt gctgcaagtt tgacacaagc attgaccact
    13141 tattttcagt aataaataag cccagccaaa caaaaatagc tcctcatatt tttcgtttgg
    13201 ccgggcttat tatcgcagat gcaagctttt gaacaccgtt taaggaagag gttgtgagcc
    13261 tgccgtttaa ctccgcgtca caaggagcaa tttcaaaaaa cagcttctca tgtttgatga
    13321 ctattgtgac ttgtgaccga ggagttggca tgagaacacc gtttattcgg aacaagggac
    13381 tgtgacatga cttttgtcac agttccatta tttttttagc agcttcaaag ctttttctgt
    13441 attggcaaaa tgaagtttgg catatttttc aagcataggc atgccgcctt tctccaatag
    13501 ttttgccgct acttggtcag atttcgttcc agctcctgat ggaagggtga ttccagcttc
    13561 agctgatgct ttgtgtaact cttccaaaaa gctggcacga ctgatgatag aagcagcagc
    13621 aacagctaaa tgatattgtt ctcctttagt aacgaagtac agtttttctt gtaattgatt
    13681 tttttctaaa cgcgcatatt ttttaaaatt tgcttccggc gtaaactgat cgatcaaaat
    13741 cgcttctggt ttttctggct cgattttttt tagcagcaaa tggatcgctt gattgtgcaa
    13801 agcgactttc atccgaacgg cattgtattt tggttggatc tcattgtatt tttctggaga
    13861 taagacaagc agtttatagg ggatggtttt ttttaagatg tcagcaagct ggatgatttg
    13921 gctatctttt aactcttttg aatctctaac accaagttta cgtaattctg caagctgatc
    13981 acgtttcaca taagcagcac atactgttag cggaccaaaa tagctgccat ttccgacctc
    14041 atcactacct aaaacggata attgactgat attttttggc aaagaagagg ctgtttttgc
    14101 tggcttggct tgtttatcct gtgcctcccc ccatttatca gcttctattg ctgcattcgt
    14161 accttgaaac atcacttttc cggatgtata agcggtaatg gtcgtcccgt ctttttttgc
    14221 agcaaagcta gtataaggaa tctgtttatt taacaaataa gagcgatagt atgctttcat
    14281 ttgactaagt tggtcgtttg ttgctttgat aatgacgttg ctcataaaat tctcctcgtt
    14341 tttatacata aaactatacc gcaactcacg acttttcgtt ttctaagaga ttgcgagaca
    14401 tagcattttt ttaaagttta tcatataata gttaagtatg tgaaaaatat ttgtggagaa
    14461 cggtgaaaga accaacttca tggtacacta aataaaactt tcatcaaata ggaggaaatg
    14521 attcgtatgg cgcacgaaaa aacacgctac aaagcagtga ttgccaacca aacatatact
    14581 attatcggac gggaaaccaa gcatcatatg gatatcgtaa cgaagctgat caatgaacag
    14641 ttagcagaat tgaaacaatt atcaccacaa atggataatg aacaagcagc aatcttgatg
    14701 gctgtcaatg cgctttcgga tcaattgaag aaacaagaaa gaatcttgga gctggaagaa
    14761 gaaacagcag aattaaagaa aaaaatgatc aaatttacag agcttgaaaa tcgtgtgaaa
    14821 cgcattgaag caatcgagaa cgaagctcgg gaagtattga aagaaaatgg tcaagcagat
    14881 cataccatta aaaatcatgt ggaagcacaa cagattttaa acgagaaacg aaaaggacaa
    14941 attaaacaaa aatcttctaa ttaagatcct tttatcaaaa taagaaaaat ttaaaaaagt
    15001 tagaagagaa aggaacgtag catgctgagt ttgctgattt tatttttatt gctaattgcg
    15061 tttttttctg gagcacgtcg cgggtttgct ttgcaaatca tattcgcgat tggatatgta
    15121 ttgtcattta ttgctgccca gcatttttac aaaccattag caagtcattt ggaattatac
    15181 attccatacc cagctgtcac cccaacatca aagttagcat tttttgatca ggtatttgct
    15241 tttcacctag atgaagcttt ttatgcagga actgcttttc tgattatttt gctaattggc
    15301 tggctgctta cccgatttgt gggggttttt gtccatggat tgacgtatgt gccgatcttg
    15361 agacaggtag actggattgc aggaggcatc ctgtcactaa tcatggctta cgttactata
    15421 tttttgattt tgcagttact tgcctttgtt ccattagtgt ggtccaaaat caattcggcg
    15481 agcatagttt cgctcgtttt atcgttgaga gaacgccatt tcttaccaac aagatgtatg
    15541 agctttgggt aacgaatatt ttgaattaag gaaaatagtg ctggaaaata tttcgttttt
    15601 tgtacagagt agtttattta agaaggctgc gtagcgtatg tccggcacag ctcttttttc
    15661 atagtgaaag ataaggtgat aaaatgaata accgaatcct tgaaacattg gaatttgaaa
    15721 aagtcaaaca gatggtcaga caatttgtag tgactgcaca aggaaaagaa gaactggcag
    15781 aattggtacc agtgagtgag aaacaaacga tcacaaactg gcttcaagaa actgaggatg
    15841 gattgaaagt gcaacgtctg cgaggcggga tccctatacc taaacttgaa aatatccgtc
    15901 cccatatgaa acggatcgag atcggagcag acttgaatgg agtggagctt gcacaagttt
    15961 ccagagtatt gtctacgaca agcgaattga atcgtttcat tgatgatctt tctgacagtg
    16021 aaatcgaatt tgcacgtttg tatatgtggg cagatcagtt agtcacgatc cctgttttga
    16081 gccgccgttt aaaggaagca atcgatgaag acggacgcgt gactgatgac gcttctcctg
    16141 agttaaagag cataagacag aacattcggc gaagtgaaca agctgttcgt gaacagttag
    16201 atgggatcgt ccgtgggaaa aatgcaaagt atttaagtga tgcgatcatc accatgcgaa
    16261 atgaccgcta cgttatccca gtcaaacaag aataccgcgg tgtatttggc ggtgtagtcc
    16321 acgatcaaag tgcttcagga cagactttat ttattgaacc aaaacaagtg gtggatttga
    16381 ataatcgttt gcgtcaatac caaatcgctg aacgaaatga gattcaacgt attttaagtg
    16441 aactttctgc tgaacttgtt ccccatcgtc aagaaatcat ccacaatgct tatgtgattg
    16501 gtaaaatgga tctgatgaat gcaaaagcac gatttggtaa agaagtaaaa gccatcgtgc
    16561 cgggaatcag tgaagacaat catgtcgtct tgaaacaagc aagacatcca ctgatcgatc
    16621 aggaaaaagt agtttctaat gacatcacaa tcggaaaaga ctatcaagcc atcgtcatta
    16681 caggacctaa cacaggtggg aaaacgatca cgttgaagac tttaggtctg ttacaactga
    16741 tggggcaagc aggattaccg attcctgcag gtgaagaaag tcaaatcggt atttttgagg
    16801 aagtatttgc ggatatagga gacgaacagt cgattgagca aagtctatcg accttttctt
    16861 ctcatatgac gaacactgta gatattttat caaaagtcaa tgaaaaaagt ctggtattat
    16921 ttgacgagct cggtgcagga acagatccgc aagaaggtgc tgctctagca atcgcaatct
    16981 tagatgatct tggtaaaaag tctgcttatg tgatggcaac aacgcattat cctgaattga
    17041 aagtttatgg atacaatcgg gcaaatacga ttaatgccag catggagttc gatgtggata
    17101 cactaagtcc aacgtatcgt ttgctgattg gcgttcctgg acgaagcaat gcgtttgaaa
    17161 tctctagccg attaggtctt gatacagaag ttattgatga agcgaagcag ttgatgaatg
    17221 atgagagcca agatttaaac gaaatgatca cagatttaga gaatcgccgg aaaatggcag
    17281 agaccgaata tctggaaatg cgtcattttg tttctgaggc acaagaactt cacgatgatt
    17341 tgaaagaagc ttacagctat ttctttgaag aacgtgaaaa agaaatggaa aaagcaaaga
    17401 aaaaagcaaa cgaagtcgtg tcagaggcgg aagaaaaagc agaaaaaatc attgctgata
    17461 ttcgtaaaat gcaacagcag attgggcaag gaaatgtgaa agagcatcag ttgatcgatg
    17521 caaagaccca attagcaaat ctacatcaag aagaaacttt gaagaaaaat aaagtattga
    17581 agaaggcgaa agaacaaaaa acgttgaaac cgggagacga agtgttggtt acgacttacg
    17641 gccaaagagg gacgctttta cgtaaaaacg ggaatcagtg gcaagtagaa atcggtatat
    17701 taaaaatgaa tgtgtccgaa gacgagctga cgcctgttgc tcctcaaaaa gagccgacac
    17761 aacgagttat ccatgctgta cgatctgaat ctagtagtca cgtgccgaat caattggacc
    17821 ttcgaggaaa acgatacgaa gaagcgctaa gtgaggtaga tcaatatttg gattctgcca
    17881 ttctagctgg ttatcctcaa gtgactattg tccacggaaa aggaacaggc gcacttcgca
    17941 agggaattac tgattatctt aaaaaccatc ggagcgtcaa aagctttgag tttgcaccgg
    18001 caaaccaagg cggaaatggt gcgacaattg ttaaatttaa gtaaagctta ctaaaggtaa
    18061 gcagatggtt cgctatccaa gtatagaaat gctatactaa tcatagattt taaaaatgga
    18121 ggtctttgat atgtcacaag taattacaga taagacattt aatgaagaaa cagataaagg
    18181 acttgtattg attgattttt gggcaacttg gtgtggtcca tgtcgtatgc aagcaccgat
    18241 tttggatcag ttggaacaag aatatgatga agaagaattc agaattgcta aaatggatgt
    18301 ggatgaaaat ccagaaactc ctcaacaatt cggtatcatg agcattccaa cattaatgtt
    18361 gaaaaaagat ggtcaagtag tagaaaaagc tgttggtgtt cattcaaaag aacaactacg
    18421 tcaaatgatc gatcaatatt tataaaaact tacagcaaaa ataagctgag ggtgggactg
    18481 aatccagttc cgtcctcggt ttttttgcgg ttgagttaag gttattcaaa ttaatatttt
    18541 ttgagtataa ttacgttgtc aggctggatt gtttttttga cagacatgct ataataactt
    18601 taattgaata tgaaggggag taccagatga acgaacgaat caaaaacaaa ttagctttac
    18661 ttcctgatca gcccggctgt tacttgatga aagataaaaa tggaacgatc atttatgtcg
    18721 gcaaagccaa aatattaaaa aatcgtgtcc gctcatattt tactggcagc cataatacga
    18781 aaacggaacg gcttgttagt gaaatcgaag actttgaata catcgttaca gaatcgaata
    18841 tcgaagcatt gctcttagag atcaatctga taaagaaaaa tgatcctaaa tacaacatta
    18901 tgttaaagga tgataaaacc tatcctttcc ttaagataac gaatgaaaaa tatccccgtt
    18961 tggtgattac tcgtaaagta ctaaaagaca aagcactgta ttttggacca tatcccgatg
    19021 tcggtgcggc gaatgagaca aagaagatcc tcgatcgatt atttcctctg agaaaatgca
    19081 aacctagtca gacgaaggag ccttgtcttt actatcattt gggacaatgt ctgtgtcctt
    19141 attatttcga cgtagatccg aaagtctata cgaaaatcgt tgatgaagtg aaaaagtttt
    19201 taaacggagg acacgaaaaa atcgagtcag agatccatca gaaaatggaa aaagctgctg
    19261 aaaacatgga gtttgaaaaa gctgcagaat accgagacca gatccgtgcc attgagacaa
    19321 tcatgactcg ccaaaaaatg acaaatactg atttactgga tcgagatgta tttggttatg
    19381 cgattgacaa aggatggatg tgtgtccaag tgtttttcgt aagacaagga aagctaattg
    19441 aacgagatgt ttcaatgttt cctttctata acgaagcaga agaagatttc ttgacttacc
    19501 tcggtcagtt ttatcaggaa aatgagcatt tcattcctaa agaagtcttg attcctgata
    19561 cgatcgacaa gcccagtgta gaagctttgt tagctacgaa agtgctgcaa ccaaaacgag
    19621 gagaaaagaa aaaattagtt gaattggcga ataagaatgc ctcagttgct ttgaatgaac
    19681 ggtttgatct gattgccaga aaacaggaac ggacgatcgg agcagttgaa cgtttaggaa
    19741 acgcgatgaa tatcccgacc ccaatacgta tcgagtcttt tgataactct aatatcatgg
    19801 ggacgaatcc tgtggctgcg atggtcgtat tcattgatgg aaaaccggca agaaaagaat
    19861 atcggaaata taaaataaaa acagtcaacg gaccggatga ttatgcatca atgagagaag
    19921 tcatctatcg cagatattca cgagtgattc gcgaagggtt gcctttacct gatttgatct
    19981 tgatcgatgg aggaaaagga caagtagatg tggcaaaaga tgtcttagaa aatcaattag
    20041 gaatcgatat tcctattgct ggcatggcaa aaaacgataa acataaaaca aatgagttgc
    20101 tattcggtat agatttggct gtagtaccgc ttgagcgaaa ttcaccagag tttttcttac
    20161 tccagcgaat ccaagatgaa gtccatcgat tcgcaatcac tttccatcgg cagctacgca
    20221 gtaaaaacag ttttgcttcc cgattagatg aaattgaagg gttaggacca aaacgaaaaa
    20281 aaatgctgct taaagagttc aaatcgttga aaaatatcac agcagccacg acagaagaat
    20341 tacaagaaat cggactgccg aaaaatgtcg cacaaaacgt atttgataaa ttacatcagg
    20401 aatgaggaaa gggcacttta caaagaaagt aaagtgtcct atcattttat gtacaacaaa
    20461 aagagcgttt acgttttgtt ggtaaataat aaatcgctta cgtctggtgt ctaaggatat
    20521 atgtgatttc ttgaagaagg agtgaagaaa tgttagaagt caaggatctt gttaaaactt
    20581 ttgggaacta cacagctgtc gatcatgtct ctttccaaat ccccgatgga aaaatcatgg
    20641 ggctaattgg ccaaaacggg gctgggaaga caacgacttt tcgtttgatc ttggattttt
    20701 tgacacaaga ccaaggagaa gtgttatgga atggccatca tcttggggag aaagattatg
    20761 atgtcatcgg ttatcttccg gaggaacggg gactttatcc gaaagtaacg attcaagacc
    20821 aactgcttta ttttgcagaa cttagaggga aatcaagaaa agagattgaa ccaaaaatcg
    20881 atttttggat ggaaaaattt caggtaaaag ggaaaaagac ggataaagta aaatctttgt
    20941 ctaaaggaaa tcagcaaaaa gtccagctga tcgccacgtt aatccatgaa cctaaattga
    21001 tcattttgga tgagccattc agcgggttag atccggtaaa tgctgaattg ctaaaagatg
    21061 gaatattgga attgaaaaaa catggttcat gtgtcatttt ttctagccac aatatggata
    21121 atgtagaaaa aatctgcgat catttgatca tgttgcgaaa cggaaaaatg gttttgaatg
    21181 gaaaagtaca tgagattcgt gaatcatttg gccgtacgaa gttattcttg gaatctggtt
    21241 taagccaaca ggaagtagaa gaaatcgaag gcgtgaaaaa agtgatcctt catgaagaca
    21301 atacgttaga gattacctta gaagatcctg aagctgggaa agaaatcttt actcgtgcga
    21361 ctcagttcgg ctacattcct atgtttaacc agcaaccgcc aactttggaa gagattttca
    21421 aattgaaagc aggtgaaatc gatgcgtaag ttttggatta ttaccaaaga tgtctattta
    21481 aaaaatgtta agtcgatttc tttcttgatc atgatattag tcccttttat catgctgggt
    21541 atcatttatg tagcaggaaa ctttgctcaa caaaatagtg atgtcaataa aatcggcgta
    21601 ttggctgata atgagcaaat cagtcagcaa atgagccaag tcaaaacaga tgattttgag
    21661 ttcaaagcag ttgattctga agaaaaagca caaaaagagc tttcggaaga aaaaatcgat
    21721 gcgtatttag tagtctcaac tgagaatgat aagataaatg ggatgcttta tagcgaaaat
    21781 tcattaggac aaagtacaca actactgata caacaacaac tgactggctt gcaatcgatg
    21841 cttcgagcaa gtcaattagg catttcacca gaagaagtag ctagcttgag ccaacaagca
    21901 agtttgactc gacaaaaagt caattttgat tcaaaaggaa aaatgacagt tggagaagat
    21961 aactcgacag tccaatatgt cgtaagttat gtagcaacga ttgtcttgtt cattattatt
    22021 ttgacgtatg cgcagatcat tgcacaagag atcgcttctg aaaaaggcac gcggatcatg
    22081 gaagtgatcc tgtccagtac gactgcacaa aagcattttt acggaaaact aacaggtgtt
    22141 ttacttgttg cattaacgca aatggcatta tacggtatta tttttggtat aggatacagt
    22201 caattcaaag atatggaaat ggtcaaaaat atattagacg gtatctcatt ggataacttg
    22261 attggaccat tcttgtggta ttctcttcta ttcatgatct ttggcatttt gattttttct
    22321 gttttagcag ctttatgtgg ctctttggtc aacaaagcag aagatacagc aaaagcaatt
    22381 ttgccagtga cttatctatc tctaggcgga tatatgcttg gattgatact aggagcttca
    22441 gatccaaaca atatcgttat tcggatcact tcgtatattc cattcttatc ttcatatatt
    22501 atgccggttc gcttagcaaa tgagacagtc ggtatgggtg gagcattgat ttccttgttg
    22561 gtacttgtag ccgtaacatt cttattgatg atcttgtctg caaacatgta caaatcgaat
    22621 gtactcgtgt atagcgaagg cggattatgg agttcattga aacaatcgat ttcaattatg
    22681 agaaatgaac gaaaaaaagc ataaaatata gaaaaatata gaaggaagct ggacatttat
    22741 tgttcccagc ttcttttttg tatagaccta tttctatttt tttattatat tggatatata
    22801 tattcattga aaatagagca taacaacttt ataatgttga gtataccaat ttttggaggc
    22861 gttttaatgg accgctattt agatttactt tcagagaagt tccctacaac ggaagatgtc
    22921 atcacggaaa tcatcaatct cgaagcaatt ttgcagcttc caaaaggaac agagttattt
    22981 ttgagcgata tacatggcga atttcctgct tttgatcata ttctgcgtat cggttcagga
    23041 aatcttaaag aaaagatcaa agaccttttc tctgaacaaa tgacggaaac ggagatcagc
    23101 caattcacta tttttacggc ttatccagaa tatgtgttga cgactgattg gtacaaaaaa
    23161 caagataaat cccagctgat ccatcgtttg atcgatcttt tacggtttac gacagtcaaa
    23221 tacactcgat caaaagttcg aaaaggattg ccgaaagaat atagctatat cattgaggaa
    23281 ctcttgtata tcgatgatcg tatcaacggg aaaaaagact atgtaaaaaa aatcattgaa
    23341 caacttgtct tgatgaagga agaagaaaaa tttttaacaa aacttgcgca gacgatccaa
    23401 aaatctgtca tcgatcattt gcatatcgtg ggtgatattt ttgatcgagg aactcaagca
    23461 gccaaagtaa tggatcgaat catggatttt tcctctgtag atatccagtg ggggaatcat
    23521 gatattttgt ggattggtgc gtattgcggc tctgaggcat gtttattgac gcttttacgg
    23581 attgccgcac gctacaacta tttatttgaa ttagagaaag aatatggatt aaatttacgt
    23641 ccattatttt cttttgccca tcaaacctat cagaagaatc cagtatttac ccctaaaaac
    23701 aggaaagatc tatctgaaag agagcaagaa gaattagaaa agatccatca agcactttct
    23761 atcctccaat tcaaatcaga acatcagttg ttgaatcgac gacctgaatt taaaatggac
    23821 gatcgtaaat tgttggaaaa aatcaactat gaggaatcaa cgatcgtttt agaaggtcag
    23881 ctctatgagt tgaaagggac ttgttttcaa acgatccagc cagatgatcc taaaaaattc
    23941 ttggaagagg aacaaaaagt gatcgatagt ttgatgtatt cattccagca ctcgttaaga
    24001 atgcaaaaac acatgacgtt tttgatcgaa aaagggggaa tgtatctcac gaacaatcag
    24061 catgtactgt tccatggctg tatacctgta gatgaaaagg ggcagctctt agcatttgaa
    24121 ttagaccaat cttatcgcgg aaaagaactg ctgggatttt ttgaaaaaca gataactgaa
    24181 agcgcaaaag atttgactgc aaatgaagat ttggcgaccg atttgatttg gtatgcgtgg
    24241 agcgggccat tttcgccttt attcggcaag agtaaaatgg caacttttga gcgctatttt
    24301 ctcacagagc ctcatacgca cgtcgaaaaa agcaatccct attatcactt gcgagatgaa
    24361 gagtggttca gtgaaaaaat tttggcagaa ttcgatgcaa aagaagaagg ttcagcaatc
    24421 atcaatggac acacgccagt aaaagtaaaa aaaggagagt caccgatcaa agcaaacgga
    24481 aaagcatttg ttattgacgg aggtctatcc aaagcttatc aaaaaacaac cgggattgct
    24541 ggctactcat tactatgcaa ttcttacggt ttccaaatcg tgacacatta tccgttcttg
    24601 cctattgaac aacaatttgc aaaaaaatct gatcaaacga atgtaaaaaa agtgatcgag
    24661 cagcagctcc cacgcaaact taatcggaat acgacaaaag gaatggaatt acagcaacag
    24721 attactcaac tccaaaggct gttggattat cgaaaggacg attgagaaaa atgctagcca
    24781 acattgctca atagactaaa aagcgactgc aacaaaaggc gtgtcgcagt ctccttcgga
    24841 attagctcaa aaaacggtaa aatattagaa gctatttttc tgtttatttc tctaatactc
    24901 aggtcagggc gctgatttca cgacctcttc cttaaacggt gttctcctgc caactcctcg
    24961 gtcacaagtc acaatatcca acaaacatga gaagctgttt tttgaaattg ctccttgtga
    25021 ctcagagttg aacggcaggc tcacgaccta atcagaaaaa cgattaaaat tcattcacaa
    25081 agaaatcgaa taaacgctgt tcagatgaat ctacacgatg agaaagttcc ggatgccatt
    25141 ggacaccaag gattttgtgc atattatctt tactttggac tgcttcaatg agtccatcgt
    25201 tggaaaaggc gatggctttt aacaaaggag aaagatcttt gactgcctga tgatgatagg
    25261 aattcacaaa gtatgaatca gggagaaggt cacttaataa actgtccgaa acgattttga
    25321 cttcgtgagt tgcaaactgc ggggcagtgg gctgttgttc gtgttttacg gaccattttg
    25381 gatataatga aagatcttga taaagtgttc cctccaacac gacattcaat agttgaagtc
    25441 ctcggcagac tgcaaaaatc ggttttcttt gtttcaatgc ttccaagatc aatgcctgtt
    25501 cgaattggtc tcgttgaata tttgtttccg tcagttttgg atgagggtct tccatgtaaa
    25561 gttgcggact aacatcctga ccgcctgcaa gaatgagttt gtcgattttc tcaattagtt
    25621 gaggcgctga atctggagac atgactggca gaataagagg taatccttga gcatctaaaa
    25681 ctgcatccac aaatccctgc ggtgtatagc ttacttgatt tccttggaaa gtatctgtcg
    25741 ctcgaatcag ttgattcccg gcaatgccga tgattggttt catttttatt ccccctaaag
    25801 tagactaatt gtaacaagga tttaaaaaaa tttaaactat gtaagacttg cccctatact
    25861 tatctgttct tgctgtgtta ctatctgtaa agagaatttt cgtgtgaaat tcatgacgat
    25921 agttgaaaat attgcttaat ttgttataat aaaaagtaag cagaaataaa aaggttgggc
    25981 gtaagcttca aggatctagt tcttcaaggg gtgagaagga cattttattg aaggggcctg
    26041 cctatactaa gttatcttta gaggtacata ctttcaatga aaaaagaaag catgtacctc
    26101 ttttttaaat cgaaaggaag tgaagcaagt gttagaagct ttggaacaaa tccgaaaagc
    26161 agaagaagaa aatgagaagc gtgctcgaac cctacggcag cagttgaaga cctatgcaga
    26221 agaaaaagag caagaattgg agataatcca aacgaacagt cggcaacagc tgaaacagtt
    26281 ggtagaagaa aaagaacaat tcgaaaagca agaattagaa aaagaaaaaa tgaaattgaa
    26341 agcagaagca gacaaggtgc aagcaacttt gaaagaccaa tatcaaaagc atcagagtca
    26401 agcaatcggt gcaatcattg aaagggtgaa agaaacttat ggccgtcact aagatggaaa
    26461 aagtgaccct catttcagat aaaagaaatc aggagaggat tttacaagca gtacaaggaa
    26521 tccaaaatgt tgaaatccgg gatctgtatc aaaatagaac aaacaaccag tgggtagaaa
    26581 aatattttcc agataccacg attattgata aagaagcaaa attaagcgct ttaggcaatc
    26641 ggctgaaaga catccaagaa gctatccaat tcatacaaca ccacggcgat agccgtcaaa
    26701 agaaactcca tttgaaacgt caagaacttt ccttacaaga cttggaagca aattattccg
    26761 aagaaaattt catgaataaa ttagaagaag tccttgattt aaaagaaaag tgggaacagc
    26821 taactgaaaa acgtgatcgt ttagaagaag aggaagaatg gcttcttcat tggcaacagc
    26881 tggatattgc tccaagtatg tatgcaagta agacgaccat ttttgagatg gggactgtga
    26941 gtgcagcaaa ttttgaaagt ttcaaggaag aactaagtaa gcttgatgac gtttatatgg
    27001 aagaaataga ttatacgcca aaacaagttt atctttcgta tgttgcttta aaaaaatcag
    27061 ttgctgctat agaagaaatt gctgggaaat acgggttcaa taaagaaacg tatccttatg
    27121 aacaaaatcc taaagaaaaa ttgcaagaaa ccaaacagca attatccatt gttcacgagc
    27181 agcaaaaaaa gataacgaca gcgttaggtc agtgcagcgg ctatatccaa gaatttgaat
    27241 gggtagaaga agtcacactt gccgttgcag aacgggaaaa agtaaaagaa cggtttatcc
    27301 atgccactta tctgatcgtt ttgcaaggtt gggtagatgc agaagaaaaa cagaatttga
    27361 tgcatgtgtt aaacgaaact gtatcagaag aagatatata cgttagtttc gaaacaccaa
    27421 actcagaaga gattcagcag gaagtcccta ctaaattgaa aaaccatccg cttatcgaac
    27481 cttttgaatt gctgactgaa atgtacagtc tgccaaaata tgaagaagta gatccaacgc
    27541 cttggatgac accattttat cttgtcttct tcgggatgat ggtcgcagat gttggttacg
    27601 gattgctgat gctcattggg acaatactag ctcaaaaact atttgtttta ccccgcggga
    27661 tgaaacgttt cgtaaaattc tttgagattc tctcggtacc atcgatcatc tggggattga
    27721 tctacagttc atttttcgga caggcattac caaaagaact atttggtatc cgcttgcctt
    27781 tcccaatcct atctacgacg gatgacgtca atacgatttt gattttatcc gtgattttcg
    27841 gtctgataca gatcttggtt ggattattcg tggcagcaaa ggaaaatatc aggaggaaag
    27901 attacttgag tgctgtcagt gatggttttg cctggcaagg gatcctgatt gggatcgtta
    27961 ttgcattgat tggtgtcatg ttgataaaaa atacagcatt tgtttatctt ggcgcggggc
    28021 tatcgatcat tgctgcttta tgtatcttaa tcgtgccgat tatccaatcg ccgtctaaag
    28081 tcaagggtgc tgccaaagga gcgtataatc tttacggatt gactggttat atcggtgatt
    28141 tagtcagtta tacacgtttg atggcactag gtatttcagg cggaagtatt ggcgcggcat
    28201 tcaatatgct agttgctttc atgccgccag ccgctcgatt cagtgtaggg atccttttga
    28261 tcatcgcttt acacgcactg aatatgtttt tgacactgtt aagtgcctat gtccatggtg
    28321 cacgattgca gtatgtcgaa ttttttggaa aattttatac aggaggcgga cgtgcctttg
    28381 acccgctgaa aacggcggaa aaatacgtca acatcaatca taagaaaaaa aacaataaat
    28441 aattggagga tttttataca atgatggatt acttaattag tcaaaatggt ggaatggtat
    28501 ttgcagtatt agcgatggca acagcgacga ttttttcagg gattggttca gctaaaggtg
    28561 ttggaatgac aggtgaggca gcagcagcgt taacaacaag tcaacccgaa aaattcggac
    28621 aggctttgat tctgcaattg cttccaggta cgcaagggtt gtatggattc gttatcgcct
    28681 ttttgatttt tatcaactta ggaaatgata tgtcagttgt acaaggattg aactttctag
    28741 gcgcttctct gccgattgct tttacaggtt tgttttcagg tatcgcccaa gggaaagttg
    28801 cagcagcagg tatccaaatt ttagcgaaaa aacctgaaca tgctacaaaa ggaatcattt
    28861 ttgctgcgat ggttgaaaca tatgcgattt taggtttcgt tatttccttc ctattagtat
    28921 tgaatgccta agcataaagg aggaaaagca gatgaacgcc attgaaaaaa tcatctctca
    28981 gatgaatgaa gcagcagagc aagaaagagc ggcgttagag caagaagagc gaatgaagat
    29041 cgatcaaaac tttgaacaaa aacgtacgca aatcgaaaca gaacatcaaa aacaaaaaga
    29101 aaaacaaatc gaattacttg aaaaaaaata ccgtcagctt aggaatcgcc aacaagtaga
    29161 agtacgacaa gaaaatttga atgcgaagca ggaattttta cgacgattgt ttgcagatgc
    29221 aatagctgaa atggagaatt gggatgaatc tgaacaaatc cagtttatca aaaattcatt
    29281 gtattcatta cctttgacag gcaaagtaac attcattgct ggagaaaaat cagcagctta
    29341 tctttctcag acattgctgg atgaatggaa caatgaactt ccctttatga tggttttaag
    29401 tgatgaaact gttgctgatc aagcgggttt tctgataaac gatcaaggag ttcagtataa
    29461 ttttcttttc agcagccttg tacaagatat ccaaggaacg atgagttttg agattgccaa
    29521 tcaattattc gaataaggag ggatcagtgt atggaatacc acgaactaaa tcccttgatt
    29581 cgcggaagag agcttgaact tttgtccaat gagacatttg aacggatgat tcaaacaaat
    29641 tctgtcgaag cacttggtga aatattgaaa tccacgattt acagtccata tatttatgac
    29701 ggatatgaat atgacttcga acagaatctt ttaaaggagc agagccgtct atttcgctgg
    29761 ttaaaggaat cagcaccgga gcctgaaatc gtttggatct atacgatgcg ctatactttc
    29821 cataatctga aggttttgac aaaagccgag atgacaaatc aaaatctgga ccatttgtat
    29881 atcgacgatg gattttattc attagaaacc ttgaaggatg ccattcatac gcaagtttca
    29941 aatgaactcc ctgaaagcat tatggacagt atacgagaag tacgtgatta ttttgaagag
    30001 tctaccattc ttcagggaat tgacgtgatt tatgatcgac agtttttgac agagcagcga
    30061 cggcttgccg ataagctagg atatcaagaa ttgctgacag aagtcattgc ttttattgac
    30121 ttgaccaata tcacaacgat ggctcgagga atattgcaga aaagaacata tgggttcatg
    30181 acgacggtac tttccagctc gggaagtatc gaaaaagaag tatttttgaa ctttgtagat
    30241 aaagatatgg aagattacac gaattttcta ttagagacag attatgctga ttttctgcgc
    30301 cctgtcatcc gtgagggaaa tcttgatctc gtaaagcttg aacagttgaa agatgattat
    30361 ctaagttcat tgtatcaaga agcacaaacc caggctttcg gaccactgcc actactagcc
    30421 tttttgaacg caaaagaggt agaaagcaaa aaccttcgtt tactggttat cggcaaaaga
    30481 agccatttcg aaccagaaac aatcagaaag cgggtgagac aagtctatga cgcataagat
    30541 cggtgtagtt ggcgacaaag attctgtttt gccattcaaa ttgtttggct ttgatgttcg
    30601 ttatggtaca accaagcaaa ccgttcgtca ggcaattgaa gaaatggcaa agaatgagta
    30661 tggcgtgatt tatgtcacag agcaatgtgc agcattgagc ccagaaacga tcgaacgtta
    30721 caaggaacaa atgatacctg ctatcgtatt gattcccaat catcaaggat cattagggat
    30781 agggctggaa gaaattcaaa aaaacgtaga aaaagcagta ggtcaaaata tcttatagta
    30841 tgaaaaggag atagaaattt tgcaaattgg aaaaatcata aaagtctccg gacctcttgt
    30901 aatggctgag aacatgtctg atgccagtat ccaagacatg tgtctagtgg gagatctagg
    30961 agttatcgga gaaattatcg agatgcgtgg cgatgtggcg tctattcaag tatatgaaga
    31021 gacttctgga attggtccag gcgaaccggt tcgctctact ggtgaagctt tatctgttga
    31081 attaggacca gggatcattt cacaaatgtt tgacggaatc caacggccat tagatacctt
    31141 tatggaaatc acacaaagta atttcttagg gcgcggagtt caattgcccg cattggacca
    31201 tgagaaaaaa tggtggttcg aaccaacagt agaagccgga gaaacggtga gtgctggaga
    31261 tgttatcggg atcgtagaag aaacaaaagt tataaaacat aaaatcatgg tgccaaatgg
    31321 gataaaagga acgatcaaaa caatcgaaaa aggttcattc actattgatg aaacagtatg
    31381 tgtcattgaa accgaaaatg gtgacaaaaa tttgacgatg cttcaaaaat ggccagtaag
    31441 acgtagtcgg ccaatcaaag aaaaattaaa cccggatgca cccatgatca ctggacagcg
    31501 agtgattgat acattcttcc cagtgacaaa aggaggagca gcagctgtac ctggtccttt
    31561 tggtgcaggg aaaacagtcg ttcagcatca aattgcaaaa tgggccgatg tggacatggt
    31621 tgtctatgtg ggatgtggcg agcgtggaaa tgagatgacg gatgttttaa atgaattccc
    31681 tgaattgatt gaccctaata caggagaatc gttgatggaa cgtacggtat tgattgctaa
    31741 tacatcaaac atgccagtag cagctcgaga agcttctatc tatacgggaa tcacaattgc
    31801 ggaatatttc cgtgacatgg gttatgacgt tgctatcatg gcagactcca cttcacgatg
    31861 ggcagaagca cttcgtgaga tgagcggacg tttggaagaa atgcctggtg atgaaggtta
    31921 tccagcttat cttggttcac ggcttgcgga atactatgaa cgatctggaa gagtcattgc
    31981 attaggatca gaaggtcgcg aaggaagtat aaccgcaatc agtgctgtat ccccgtctgg
    32041 aggggatatt tcggaaccag tcacacaaaa tacattgcgg gtcgtaaaag tattctgggg
    32101 tcttgattcc agtttagccc aaaaaagaca ttttccttcg atcgattgga tacaaagcta
    32161 ttctctttat tcaacagaag tagggaagta tatggatcat atccttcaag ctgactgggc
    32221 atccatggtc accgagggca tgcgtatttt gcaggaagaa cagcagctga atgaaatcgt
    32281 gcgtttggta gggattgatt ccctttcaga taatgaccgt ctgacattag aagtggcaaa
    32341 atccattcgt gaagattatt tgcagcaaaa tgcttttgat gatgtcgata cattcacttc
    32401 aagagagaaa caattcaaaa tgttgaagat gatcttgacc tttggtgaag aaggacgccg
    32461 tgcgttgtcg ttaggtgctt atttaagtga gatcatggac ggtaccgtat cgattcgtga
    32521 aaggatcgga cgaagcaaat atatcccgga aacagaatta gaaaaactag atcaaattat
    32581 tggtgaaatc aagcaaacga tgcaaacgat cgtttcagaa ggaggcatga gcaatgatta
    32641 aagaatatcg tacgatcggt gaagtcgtcg gtccattgat ggcggttgaa aaagttgctg
    32701 gtgtcaaata tgaagaatta atcgaagtcc gtatgcaaaa tggcgagatt cgtcgtggac
    32761 aagtattgga agtccaagaa gataaagcaa tggtccaaat attcgaagga acaagtggta
    32821 tcaatttacg tgactcttct gtacgatttt taggccatcc tttagaacta ggtgtatcgg
    32881 aagacatgat cggtcgagta ttcgatggtt tgggacgacc gaaagataac ggaccagaga
    32941 tcctgcctga aaagcatttg gatattaacg gagaagtaat caatccagta gctcgggatt
    33001 atccagatga gtttatccaa acaggtatct ctgcaatcga tcatttgaat acattagttc
    33061 gtgggcaaaa attaccagta ttttctggct ctggtcttcc tcataaagaa ttagctgctc
    33121 agatcgcgcg acaagcaacg gtactcgatt catcagatga tttcgccgta gtctttgctg
    33181 ctattggaat cacttttgaa gaagctgaat ttttcatgga agattttcgt caaacgggcg
    33241 caatcgaccg ttctgtcatg tttatgaatt tagcaaacga ccctgcaatc gaacgtatcg
    33301 ctactcctcg tatggctttg actgctgcag aatatcttgc ttatgaaaag ggaatgcatg
    33361 ttttggttat catgacagac atgaccaact atgcagaggc attgcgagag atttctgcag
    33421 cacggcgaga agttccagga agaagaggat atcctgggta tctttacacg aacttagcta
    33481 ctttattcga acgagcagga agaatccgcg gactaaaagg atcggttact caaattccga
    33541 ttctaacaat gccagaagac gataaaactc atccaatccc tgacttgact ggttatatta
    33601 cagaaggaca gatcatcttg actagagagc tttacaagag tgggattcag ccaccaattg
    33661 atgtactccc gtctctttcg cggttaaaag ataagggaac tggacccgga aagacaagag
    33721 aagaccatgc agctacgatg aatcagcttt ttgcagccta tgcacaagga aaacaggcaa
    33781 aagaattagc cgtagtttta ggtgaatctg ctttatctga tgtagacaag atctatgcga
    33841 agtttgctga gcggtttgaa aaagaatatg tcaatcaagg attctacacg aaccgttcga
    33901 ttactgaaac acttgatcta gggtgggaac tgctatcgat gttgccaaga accgaattaa
    33961 aacgaatcaa agacgacatg ctggacaaat atctgccaga agggaagtga gttctgatgg
    34021 cacgattgaa tgtgaatccg acccgcatgg aattgactcg attgaaaaaa cagttgacaa
    34081 ctgccacaag agggcataaa ttattaaaag ataagcaaga tgaactgatg cgtcaattca
    34141 tcctgttgat tcgaaagaac aaccagctgc gtcaagaaat ggaagagtct atgacagaag
    34201 caatgtctga ttttgtattg gcaaatgctt cgatcacgga acctttcatc gaagaattat
    34261 ttgttttgcc ggcggaaaac gttgatttgt ctatcgtaga caaaaatatc atgagtgtaa
    34321 aagtacctgt gatgaatttt gaatatgatg aacagctgac agacacacct ttggaatacg
    34381 ggtatttgaa ttccagtgcc gaactggatc ggtcaatcga tcggtttact accctgcttc
    34441 caaaactttt ggaactgaca gaaatcgaaa aaacttgtca actgatggcg agtgaaatcg
    34501 aaaagacacg tcggcgagtc aatgcattag aatatatgac gatccctcaa ttagaagaaa
    34561 cgatctatta tattcgtatg aagctggaag aaaacgagcg cgcagaagta acaaggttga
    34621 tcaaagtaaa aaatatgggt accactgact aagtggtatc tttttttaaa agaggaaaac
    34681 gatgattaaa caaagcaaac aaataaagaa acgtttgccg gctgttcagc ttctcgcatt
    34741 aggatttttt ctcctgattt tagttggcgg cggcctactt accctaccat tttttagtaa
    34801 tagtgggcat gggacaaatt ttatcgatgc gcttttcact gcaacttctg ctgtctgtgt
    34861 aacgggattg acaacattga atacagcaga gcattggaac agtgctggtc aatttttgat
    34921 catgcttttg attgagatcg gcggtcttgg ttttatgatg gttccgattc tatgctttgc
    34981 attgataaag aagaaagtca gtctcagcac acgtattata ttaaaagaag cgctgaatct
    35041 agaagaagta tcaggtgtga tgaagctcat gctttacatc ctaaaattgg cctttgttat
    35101 ccaattgatt ggtacggttg ctttatcttt tgtatttgtg cctgaatttg gctggttgaa
    35161 aggtatctgg tacagtttgt tccatgcaat ctctagtttt tgtaatgcag ggtttgattt
    35221 gttaggagac agtttagcca atcaccagca aaatgtttat ttgatcatgg tggtatctgc
    35281 tttgatcatt tctggtgggt taggcttcat cgtatggcgt gatttactgg aatatcatca
    35341 gaaaaagaag atgacgattc attcaaaaat cgcattaacc gttacaggta cccttttatt
    35401 aggcggattc gttgtgttct ttttcacaga gcgcaatggg gcaacgctag tggaagggaa
    35461 tatgtttcaa cgattggcaa acaccttttt tatgagtgtg acacctagaa cggctggata
    35521 ttattcagtt gattacctcc atatgagcca tgcaggtctg attttaacga tgtttttgat
    35581 gtacatcggc ggaacatctg ggtcgacggc cggcggattg aaaacaacta cgttaggtgt
    35641 gttgctgatc cagatgcgtt cgatgttgaa aggacgtacg agagcggaag catttggccg
    35701 taccatccga caaggcgcag tgttgcgggc acttacttta ttttttgtta cgttgacgtt
    35761 gtgtgtagtc gccatcatga ttttatcggt gactgaaacg atacctaaaa cttctggcat
    35821 cgaatatatt gcttttgagg tcttctctgc gtttggtaca gtaggtctta cgatgggatt
    35881 gacgccagac ttgactgcaa tagggaaatt gatcattata gcgcttatgt acattggacg
    35941 agttggtgta ttgactgtgg tcttctctct gatgacaaaa gcacaaagaa cacaatcaaa
    36001 atacaaatat ccagaagaaa gtgtcatgat tggataattt catcttctga cagaaaccta
    36061 gtgatttagt aaaataagaa aataaaataa tttttcttgc ttcttggata ctttaagtgc
    36121 aaaatgttcg tttatttttt gaggcactac ccgttgttga acctcgggta gtgcctcttt
    36181 tttattgaaa ggaatcaata agatttgatt cctttattta tcaataggtt ataattcata
    36241 tttttttatt ttttctttta cgatacttag tccagaagct aaaatttcaa ggttattaga
    36301 ggatagggaa attcgaacga atgcatctgg ttgcgcacgt gtagtaaagc gtctggaatg
    36361 ataaagtcgg ataccgttag caagaaagtc attctcaatt atcttagggt caatctcagg
    36421 atcaactgga agaattcgat agtaagggac acgattcgtt ggtttttcta ctttgaaaag
    36481 agaatcaaaa agctgattgg ctctttttgt aagacttaat ttagtttcta agatcctatt
    36541 tgcttcattt gaatggagga tttgagtgat gacttccaca tctaatccag aagttttcac
    36601 atttatatta aaaattgctt gttctatctt gctttggaca tgtactggaa ctacaagata
    36661 agcaactcgc aatccagaac aaataaattt tgtcattcca gctaagtaga ttgtttggtt
    36721 aggtaataaa ttttggaaag gaggaatgat cgtatgttga tagtaagttg ttaaaaatga
    36781 atggatatca tcttcaataa cccatagacc ttctttttca atcacttcgg ctaattgctc
    36841 tcgtcttttg tgagagattt gaaaccctat aggattatta caagaaggca taagaaagac
    36901 accatgaatc ttcttttttc tacattcctg aatcaatata tcaggtcgca ttccttgctc
    36961 gtcaaaatca attggaacga tctctaaatg atataactga gccaattcaa tgaaattagc
    37021 gtatgtatat cggtctgttg ctattctatc acctggagag aaaagtgcag aaagtgtgat
    37081 agccaatcca ttttgaacac cggaaacaat agatagagat tctaaggacg gaatgactcc
    37141 ttgagttttt aaccaatttg ctgcagtagt caattgaaaa tttgtgcctt tgggatcttg
    37201 gtaattaagc aaaccaacaa gatttgggtt attactagtt gattgaataa acgggacaac
    37261 gaatttatta cattcttcaa aagaactaac cagaccaaaa tcaatgatat gttcggttac
    37321 ttcattggta gagatggtga tggactcaaa gacattggga gaaataaatg tgccacttcc
    37381 aatattcgta tacagcaaac ctttctcaat tccatattta taagccttgc caacggtagt
    37441 aaaatttaaa tctaaataat cagctagctc tctttgggag ggtaactgag tgtttttctg
    37501 taatttattc tttaaaatat cttgttctaa ttgttgaatc aacgatcgat aataaggacg
    37561 cttcaatttt tgtttgtctg gtcgccaagt taacgggtaa ttgtcataag aattaatagg
    37621 cattttctcc actccttaaa attgtatacc atacaatttt accattgatt gtattgattt
    37681 tagaagttaa tcttttgtga ggagggaatt gtctatgaaa attattaatt ttgaggaagt
    37741 agtagcttct tttagttttg aagaagcgat tcaagtaatg aaaaaatgtt tttttgatta
    37801 tgaaagtggt aaaatcagcc aaaatcctcg attggtggaa attttacctg acggaaaagg
    37861 caaaaatatg tttgcgatga tgcctgctta tttgggggag aacagatatt ttggcacaaa
    37921 agtcattacc gcttttccgg ataatcatca gtatcattta ccttctcatt taggagagat
    37981 tttcttgttt gatgctcaaa acggactacc caaagcattg ataaacgcaa atgcagtcac
    38041 ctggattcga acagctgctg tatctgcgct ggcaacagat tacttagcaa atccgcatgc
    38101 agaaacactt gctttaattg gagctggtca acaagcattg tcgcatttga ctgcgatttc
    38161 taccattcgt cccatcacta cagtgtttgt ttacgatatt ttagaagaaa gacgaaatac
    38221 ttttatcgct aaagctaaaa aacagtatcc taaaataacg tttataaatg caatatcagt
    38281 agaagatgca gtacgtccag cggaaatcat ttgtacgttg acctctagta aagaggcatt
    38341 tcttcaaaaa gagtgggtaa gggaagacgc acatatcaat gctgtgggca catttactcc
    38401 tgatacaaga gagataagta catcgttgat ggaaacttct caaatctatg tagatgatta
    38461 ctcagctgcc ttaacagaaa gcggggacct gcttatagcc atggcagaag gagatttaga
    38521 ggaatcttgt attgttggta cattaggcga attagtttct gagaaaaaaa cattttataa
    38581 aggagaatcg aggccgacga ttttcgacgc agttggtcta gctattgaag atctttgttg
    38641 tgcagaatat atttataaca aattagagaa aggggcttta aaatgaactt agattattac
    38701 gtagttgatg catttgcaga tgaggtgttc aaaggaaatc cagcagcggt ttatgtgcta
    38761 gaagaatggc tgccagaagg tacgatgcaa aaaattgcca ttgaaaacaa tttatctgaa
    38821 acagcattta cagtgaagaa aaaccaagaa tttgagctta gatggtttac gccagatcga
    38881 gaaatcgatc tttgtggtca cgcgacatta gcaacagcat ttgttttatt taactattac
    38941 aaaatacctg atgaaacgat taaattttct actcaaagcg gtaatctatt tgtgacaaga
    39001 attaaagatt attattacat ggattttcct agtattatgc cgaaaaaagt gccgatattg
    39061 tctgaatatg aagaagcgat tggggctaag attaaagaag cctacttagc acgtgattta
    39121 tttttcgtat tagaagatga aaaaacggtg gctaaattgc agccagattt tacagctatc
    39181 aaaaattttg aattaggaat aggagtcatc gtgacagcag aaagtgtgca aaaagacttt
    39241 gtttctagaa ccttctttcc aaaattaaca atcaatgaag atccagtatg tggatcagca
    39301 cattctaatc ttattcctta ttgggcagaa agaatgaata aaacaaaatt gtcagcttat
    39361 caaatgtcat caaggggcgg ggatctagaa tgtgaattaa aagaagatcg tgtgataata
    39421 ggaggaaaag cagttttgtt ttctaaaggt acctcgttta tttgatttct ttcaaatgaa
    39481 ttaaaaataa attggtggag agtggcaacc ttatagctac aatggttgtt aaaaaaagac
    39541 gcaaactttt tcaatttaat gaaaagtttg cgtctcattt taattattgg tgtttgtttt
    39601 ttattcaatc aagagtaaaa tgccccatac taactatcct cggatgactt cgattttata
    39661 accatcaggg tcaatgacaa agtagtaaga aggcgcagtg ccaggtaatc cttttaagtc
    39721 agtaatctca agaccggcag ctttatgttg ctcgtgtaat ttttctagat catcagctgc
    39781 tatagcgata tgaccatagc cgtctcctag atcatacgca ccatgatcat agttgtaagt
    39841 caactctaat tcatagtcat ccccaggaag tgtcaaataa acaagtgtaa atttgttttc
    39901 tggaaaatca cggcggcggc tttcttcaaa accaaatgcc ttttgataaa attcgattga
    39961 tgcttcaaga tctttcactc gaacacatgt gtgagccatt ttcatagatt catctctcct
    40021 tttcctttta ctcaatcata acaaaaagaa gtaaggaaat agttgatttg tgcttgttca
    40081 tgagaaattt ctcatgtaaa atggatagaa aataaaactg ttaaggtgga aactattcat
    40141 ggataaacca atttttggtg aaaaaaagca atttgctgat tatcaaaccc gttatgcagc
    40201 atatattgtc atagaaagag atcagcagat tgcagtaatc gaggctccaa atggtgcttt
    40261 ttttcttcct ggcggggaaa ttgaaggaga agagacgaaa gaagaagcga ttgtccgtga
    40321 gctgatagaa gaaatgggaa ttgtggcaga aatttcttct tatctgggag aagctgatga
    40381 gtatttttac tcgaattatc gtgcaactta ttattataat ccaggatatt tctttgttgc
    40441 tgattcttgg aagcgtatag gagaaccaac tgaaaagacc aataaaattt ggtgggtgag
    40501 ccctcaagaa gcgcttgtga aattgaaacg tggtagccac cgatgggcag tcgagagatg
    40561 gatcgaaaaa aataaaaact gaaagaaagc gagtagatca gctttctctc agttttttta
    40621 tttattacag tacatatttt ttgattgctt cagctacacc gtgctcgtcg tttgatgaag
    40681 tgatgacatt tgctgcttct ttgacaagag aaacggcatt tcccattgct actcctagac
    40741 cagcatattc aatcatggat aagtcgttct cgttatctcc gatagccatg atttcatctt
    40801 gatcaatacc aaggtgctgt gcgagattcg caactgccgc acctttactt gcttctttat
    40861 ttagtacttc gaagtaatag ggcgcacttt tgaccgttgt gtatttatca cggaaagttt
    40921 gcggtaaacg cgcaattgct gcatctaaaa tttctggttc gtcgatcatc atcattttga
    40981 cgatgttcat gtctggtgtc atttcttcag gtgttcgata ttttagcggc atcttgacta
    41041 aacttgcttc gtggatcgta taagcgctga tatctcgatt aggcgtgtag atcgctttgt
    41101 catcgatcga atggagatga ctgccgacac ggcgtgccat cacttcgatc tctaggaaat
    41161 cttcatgtgt cagtccgtga cgggcgatga ttttacctga tttcgtttgt tgtacgagtg
    41221 ccccgttata agtaataacg taatcattat cttgaaaaag gttcaactcg tttaactgtt
    41281 cctggacacc tggtaaggga cggccagtgc ataaaacgat cgaaacacct tttgcaacag
    41341 cagcggcaat cgcttctttt acttcttttg ttacttctct tttctcattt aataaagtac
    41401 catcgatgtc gatagcgact agtttgatag acataatttc ctccaatata ttttaattta
    41461 tttcaatcaa tgcgccatta cggatatggc tggcaaattc ttgataagtt tcgtcaaata
    41521 aatcataatg atcacgcaag gacggatcaa ccatttcttt tggaaagaaa aatcgctcat
    41581 ctccttgttc ttgtcccgct aatgcagcaa caagctgact aacctgtgat aattctatca
    41641 aagttccgtc atttcgcaaa atctcgattt gtgtgcgatg tctgccttgt tctgggcgat
    41701 aaaaatcata aggtagatcg tagctagaat tcacagcagt gtaataaaca ggattatatc
    41761 cgaccttttc tactaacaaa gtgagttctt ggactaattt tgaatcccgg ttgccagaaa
    41821 aagtagcaga tttcagtggt ttacgattca aaaaacgttt agctaaatca tttaaaatag
    41881 gatcagcaga ttcagaccat tgagtaaagt aagtgcccaa gaccccatca tcaagtttga
    41941 ggtattcttc taaagtaaag cttccgtcaa gaaatggcag aagtaattga gaatggagtt
    42001 caaaactgct ggcatcttct tgatacaact cgtgtgcgcg atgaagcaga tgctccaaaa
    42061 ttacttccat accacgggaa acaggatgga agtacacttg cacatacatt tgatatcggc
    42121 taacgatata atcttccact gcatgcatgc cactcatgga aaaagcgatc ccgccttcat
    42181 aaggacgaat cacacgtaat attcgggtaa gatcaaatgt tccatattct gttccagtga
    42241 aataagcatc ccgaagcaaa tagtccattc gatctgcatc gatctgacta gaaatcattt
    42301 ggacaacttg tgggttgggg taagtttttt ggatcacact agctaccttt tcaggaaaac
    42361 cttcttctac tcggttaaga atctgataca cttctgtttc tggagaagtg atgatctcta
    42421 cagtgatggc ttcatgattc gtatggaaga tatgttcaaa tgtatgagag taaggaccat
    42481 gaccgacatc atgcaacagt gccgcacata aagtcaccag ccgttcctga tcatcccagc
    42541 ctccgttgcc tattttttct tttgaaaaat ttcgagaaaa gatatcgcag attcgtcttg
    42601 aaatctcata aacgcctaat gaatgagtaa aacggctatg ttctgctccg tgaaaagtga
    42661 aagaagaagt gcctaattgt ttaatgcgtc gtagacgttg gacttctttc gaattgatca
    42721 aatctaagat tacttgatgc tggacatgga tatagttatg gactggatca cgaaaaactt
    42781 tttccatagg cagcatttga tatttataag gggttgtcat aatgtagctc ctttatgtta
    42841 ttacgttgtt ccattctctg gatttggttg ttccattgct ccagctggat ttcttttttt
    42901 agccagtcgg tagaatcctg taaatcactt gcagaataca gcttttcaaa agcttttgca
    42961 aatgcttctt tgacttcttt tacaaaaagg ggttggtcaa gcagttcttc aagtgttgcc
    43021 attgtagcag gatccactgc aggatagcca tttgtaccaa agtcttcttt taaagcagat
    43081 tgataaaatc gcttcatgac ttgcccgcgt ttttcctgat taccgttgac acttaagtac
    43141 atcataacag aaatcccatt tttgatccga cgctgagcga taccggcaaa ttttttgcca
    43201 tggatgctta aatcataagt tccaggacaa taagaatggg tgatttcgaa tgcttgtatc
    43261 ggtgcctttg gaaaagcgta tttagtcaat aggagcattt gctcgtaagc gtcatctatg
    43321 gttatttttt ttgtgtcact ttgggggaaa atcaaagaaa tattcaaaat tccttcatct
    43381 gatacgatcc ctaatccacc tgaatttctg atcactggag cgtaacctgc atctttcagt
    43441 gtttggatcc cgtcacttag aaaaggagtt cgaacatcct tcatacctaa gatgactgtg
    43501 cagtcatatt gccagaaatg taaaaagatt tgctggtgtt ctttagaata ttccgttaat
    43561 atatctgtta atgcaaaagg ggaaaagtcg tttttttgat aaatgtgctg atctagtcga
    43621 atcgcagggt tcattcaacc ggctcctttg tataaattca tattttctac aatattatac
    43681 tctaaaatca aagagttgtt ttggaaaaaa ggttttctgt aaaaatggac aagttggttg
    43741 acaaaacaaa agaaactagg aacaatgtag ctatgagaag taaaggagaa tgaataatgg
    43801 aattatcaca aggaacaccg atttcaatca aattgcgtac aaaagtaaaa cagcaagggg
    43861 aagtgcaaga tttttatttt gatttgaaag ggcaaatggt gacaattggc gatacgctgt
    43921 acatacgtta taaagagatt caggaggaga cgggcgatga gattcctgta acgatcaaat
    43981 tagttccaga tggtcatgta cagctgatcc gtgcaggaga aatgcggatg cgtttgaaat
    44041 ttggctataa agaacggtta gaaacatcct atcgaacacc atacggtatg atccagattg
    44101 ctacttttac gaaagaactg catgttagtt tgaaagaccg tcccacagga gggaaggttc
    44161 gaatcgatta tgatctgttc atgggaccgg aaaaaatcgg cgaatattat ttgaccttgg
    44221 attttacagc gtaaatataa aagaaaagtt tgattttttg tgtctgattt tgtatgataa
    44281 ggagtagact gtgaaaggac gtgtcctgat tggaaattag tgtatttgaa ggtgcaaaca
    44341 aaagcgaatt atcaatgatc gaagtagcac acgcgatctt agaacaacgt ggcgatgtca
    44401 tggatttttc tgatttagct aaccagatcc aaaactatct tgggaaatca gacagtgaaa
    44461 ttcgtgattc actcgcacaa ttttatactg acttgaatat tgacggcagc tttatctcat
    44521 taggggataa tcgttggggc ttgcgttcat ggtatgctat cgattcgatc gatgaagaag
    44581 taaaccatgg aatggacgaa gaggatgaag atactccacg tcgccgtaaa cgcaagaaag
    44641 tcaacgcctt tatcaatgat gatgaagacg cgatcgatta caatgacgat gacccagaag
    44701 atacagatct gacagaagaa gatgacgatg atctgtttga tgacgatgat gatgaagatg
    44761 aagaaatcgc tgcttataat tctgacttgc aagaaatcgg agcagatgac ccgacagatg
    44821 atgaagaaga cttgccagga aatatcgaag aagatttaag tatcatagac gaagacgatg
    44881 atgatgaaga ttatgaagag gaagaatttt ctgactctga aaaagaataa acattatttt
    44941 cttacgagag gttgtgacag agacgatcag cctatcagaa taaggaggtg tgacacgatt
    45001 tttgtcacac cttctttttt gtatataaaa atcccaagta aatacataaa aaataaaaaa
    45061 attgaaactt ttagcgcacc ttgtacgtca tattaagtga ggcagaaagg agtggagaat
    45121 gatttttact ggatataaga aaaaacggca gatcgaaaaa taccgtcacc tgctgaatgg
    45181 actcatcgaa actgattatc aaaaaatgtt ccgtatcacg atgaattacg tacatgataa
    45241 agaagaggcc ttagatgtga tgcaagatag ttttcataaa gccctgaact attttgaaaa
    45301 agaaaaagag atcgagcatt ttagtgcatg gttttatcgg atattgatcc gcacggcatt
    45361 agatagttgg cgcagacaaa aaaggacgcc tgtggactta tttgaaattc aagatatccc
    45421 ccaacagtta cctttagaaa cttccgttgc agagctccat atgattttag ataaaataga
    45481 cagtccggaa aaagagattc tgatcctcta cttttttgaa ggatttcgtt tgaaagaaat
    45541 tgcgactatc ttggatatga atgaaaacac agttaaaacg aaaatgtacc gctcattgaa
    45601 tgctttacgt caaatcctag aatgaaggag agaagagtgc catgtattcc caaaaagaaa
    45661 atttaaaaaa actaaagaaa acttataaag aacaagcaat tcccaaaggg cttaaagagg
    45721 agatccgcca gcgttttatt atcgaagagc aatcatttat aaaagcaaag cggagaaaga
    45781 gacgtctcca agggttcgga gtatctttgg ctatcgtaat gattacaaca agtagccttc
    45841 tttttaatag tcaagttcga agctttgcag aagatttacc catcttagga tcagtcattg
    45901 aattgatctt aggcgaacgc tttactgatc gatctgaaaa aatcgatatt caggtaccga
    45961 agatcaatac acaaaatgag aaagaaaata aaacaattca tggattaaat caaaaatact
    46021 ttcgcgaagg acaagcagat tttgaaaaag ctgaaaaaga atatggaaat tttgaaacgg
    46081 atcattacca agttttggga gattatcaga aaatcgtaga cgataaccgg tttctcgtga
    46141 ttgaaaggca gatcacccaa acagcagcag atagccatgt tgaaaaaaga tacgacacaa
    46201 tcgacaagaa aaacagtgtc cagctctcac ttccactttt attcaaagat gacacctatc
    46261 tatctgtttt gacaaaagaa gtcaaacggc agatggccga acaagtcaag gaagatccat
    46321 ctaagtacta ttggactgag caagatattc aagaagggac gatagaaaaa ccaacacttg
    46381 ttacgcctac acgaagtttt tatttgaaca aagagcatca attagttttg actttttctc
    46441 aatacgaaat tgctcctggt tatatgggta caccagaatt tgtcattcct aagtccgtga
    46501 caaaaacgat tttggcatcg gaagattatt tggatcatta gacgagaagt agttgcgact
    46561 agcagtttca attttcaagt aagtacaagt aaataataag aagagaaaaa gagaaagaag
    46621 agatatagtg aaaagaaatt atttagttat cagtattatt gctttatccg tgcttttgtt
    46681 tttcgaaatt acttcggtgg cctccttgat caccgaagta aaaggacaaa cacactcttc
    46741 ggagcattcg tcacaatctt tcaaacaatc agaacaatca accattaaaa gccaatcaac
    46801 tgaaacagct aacgtcaaag aagccattca agaatcagcg gaaaaggtac aaagtcagtc
    46861 atcacaatca acacaagaca gtacaaaaac gagccgagat ccggctgtca aaagcgtcgc
    46921 aatcagcttt gatgatggac caggagcgac cacaacaccg cagcttctgc gaatcttaaa
    46981 agaaaaaaac gtgcatgcca cttttttcgt cttaggagaa aatacggcgc agcatcctga
    47041 aatcgtgaag cagacagctg aagcagggca cgagattggc aattacacat acgatcatca
    47101 gaatctggcc actctttctg cccagtcgat gacggaggaa gtgacgaaag cagacactga
    47161 gatcaaaaaa gtcactggca aaacccccac ttttgttcgt ccgccctatg gttctgtcac
    47221 gaatgtagga gcaacgatca tccagcgacc gatcatcgaa tggtctgttg attcagaaga
    47281 ttggaagaca agaaatcctg atttgatttt gcagaagatc caagcaacag tatacgatgg
    47341 ggcaattatt ttattccatg atatctatcc tgaaacgatt cgtgccgttc ctcaagtgat
    47401 cgattatctg aaagagcaag ggtatcgcat cacgacagtt ggggatttat tagggcatcc
    47461 aacggctgtc gagaactatt atggcagaaa cgatcatcgg ccagttcagt aagtgagaca
    47521 agtatcttta ataaataaca cattttaata agtagttgaa cacataacta ccttacttgc
    47581 aaaggcttaa caatatctat acaggtacaa caaaaaactg tccaccaatt ttctttggcg
    47641 gacagttttc acttaaaaat aaagaagcaa ctattcgtaa attatgctaa aatagcagta
    47701 gaacgcgtcc tgagggaatc gaacccccgt ctcaagaacc ggaatcttac gtgatatcca
    47761 ctacactaag gacgcaagca ctgggataat tgtaactaaa cagataaaaa attacaaggg
    47821 gattggacaa aaaaatgaaa tttgagcaaa atttttcaca aaaacagcag cagacacaga
    47881 aactagccat gacgcaacag ttgcaacagt ccatccaagt tttacaatat aacagtgagg
    47941 agctgttggc attcgttgag aatcaagcaa tggaaaatcc attggtggaa gttgttgagc
    48001 cggaatggca gccagattac ataaaagctt cttcatcttc ttatgaagga gaagagacaa
    48061 attacctaaa tcagattcct gatacaaaag ggtctttatt tgattcattg atcgaacaag
    48121 tccatttgaa ttaccgagat acttttttga ggaaaatcgt tctttatctt gtagaataca
    48181 tcgatttgaa cggatttttg acgattagtt tagaagaagc cggaaaagag attggagcta
    48241 cgcctatcca aatgctcgat gcgttgactc tgattcagca attagaacca gctggagtag
    48301 gtgcacgtag tttgcaagaa tgtctcatgc ttcaaacaga acgagatgat tatgcgccgg
    48361 aattagctta tatcgtgttg gaagaatgtt ttgaggaact ggtggagcga aaatggaaag
    48421 aaatcgcaca acgtttttct gttgatctac atgctgttca gcagatattc gactatttac
    48481 aaacgttgag tccaagccct ggccggattt tcgaccgtag cagcgaattg tacattattc
    48541 ctgatgtccg ggtattagtg gacgacgata aaaatgtaca agttatctca aaccgaaaaa
    48601 atcagccaaa tatacgcttt caagagagtt acttcaaaca aatgtcacaa caagcagata
    48661 aagaaactga aaattattta aaagaacgga aacaggaatt cgaatggttg aagaaaacga
    48721 tcctacaacg tggggataca attttgcggg ttgctcaagt cattgtttcc cggcaaaaag
    48781 ctttctttat cgacaaggaa cgtccaatca agcctcttac gttaaaggaa gttgcaactg
    48841 agatagatgt gcatgaatcc acggtcagtc gtgcagtcaa cgggaaatat ttggaaacag
    48901 atttcggtgt atttgaatta aaaaaatttt tcactacacg aattcctacg aatagtacag
    48961 aacagacaga agacctttca gcagatacag cgaagaaaaa gctgcaggaa ttagtcgatc
    49021 aagaagacaa aaacaagccg ttgtcggacc agaaattggt tgaattgctg aaaaaagatg
    49081 agatagcgat ctcaagacga acagtagcaa aatatcgtga tttattagga atcccgagtt
    49141 catcaaaaag aaaacgttat gataactaat cagtcaaaag actagaaaaa gtatttcact
    49201 ttttctagtc tttttagttg aaaaacgaga gcgaaactgt tagacttaca gtcgtgaggt
    49261 tgagatttcc ctttattgat gtgaaattcg aatagttaat ttcttttttt atcgtatatg
    49321 ggtcgtttta agtctacatt ggacgtaaag tgtccaacta aggagatcgc tatgctggat
    49381 gaaataaaaa tgattgaatc tgttgcacca gatattatcg aagtgttgca agaacgctat
    49441 aaaattttac gaaatatcta ttggatgcag ccgattgggc gtcgaagttt atcggaaagt
    49501 atggggatca cagaacgagt cttacgaaca gaaacagacc ttttgaaaaa tctgaatttg
    49561 atcgacacct caaaaagcgg tatgacgctg acaactaagg gcgaagaagt ttatcaaagc
    49621 cttgaaaact tcatggatca gcttttaggt acgcatcaaa ccgaacaaca gttggcagaa
    49681 tattttggta accaacgctg tatcgttgtt tcaggaaaca gcgaagagca gaccaaagtg
    49741 gctgatgcat tcggcgaagc gctgagcgaa gccttagacc gactacttcc agaaggcgag
    49801 aatatcatcg ctgtgatggg aggaactact atggctgtgg tagccgaaca attgtctaat
    49861 ctggaaaata agaaacggca taacctgttt gtccctgcaa gaggcgggat tggtgaagcg
    49921 ataactgtac aggcaaattc tgtcagtgca agaatggctg ctaaagcaaa cggtaaccac
    49981 cgcgcacttt atgttccgga acaattaagt ctggcaactt acaattcttt actcaatgag
    50041 ccatctatcc aagaagtctt aaacttgatt agtgaagcga attgtgttat tcatagtatt
    50101 gggcgtgcat tacacatggc agcacgtcgt aagatgactg aaaaagaatt agtcatgctg
    50161 aaacaagcaa atgctgttgc ggaatctttt ggctactttt tcaatgaaaa aggtgaagtc
    50221 gtttataagg tccctagaat cggcatagaa ttgcaagatt tggaaaaagt cccgatcatc
    50281 atggcaatcg cgggcggaaa atcaaaagct aaagcaattc gagcttatat gaaaaatgca
    50341 ccaaaacaaa cgtggctcat cactgatgaa gctgctgcaa atgagatttt aaaaggggta
    50401 accctttaaa ataaatattt ttttgatttt cataaggagg aaatcttaaa tgacagttaa
    50461 agtaggtatt aatggttttg gacgtattgg acgtctagca ttccgtcgta tccaagatgt
    50521 agatggaatc gaagtagtag caatcaacga cttgacagat gctaaaatgt tggcacactt
    50581 gttaaaatac gacacaactc aaggacgttt caacggaaca gttgaagttc atgaaggttc
    50641 attcaacgta aacggaaaag aagttaaagt attagctaac cgcaacccag aagaattacc
    50701 atggggcgaa ctaggcgtag atatcgttct agaatgtact ggtttcttca cttctaaatc
    50761 agcagctgaa aaacatttaa cagctggtgc taaacgcgtt gttatctctg ctcctggcgg
    50821 aaacgatgtt ccaacaatcg tttataacac aaaccacgaa acattaactg gtaaagaaac
    50881 agttatctca ggtgcttcat gtactactaa ctgtttagct cctatggcta aaacattgaa
    50941 cgacaaattt ggtgttgttg aaggattaat gacaactatc cacgcttaca caggtgacca
    51001 aatgactcta gacggacctc atcctaaagg tgacttccgc cgtgcacgcg ctgctgctgc
    51061 aaacatcgtt cctaactcaa caggtgctgc taaagctatc ggtttagtaa ttcccgaatt
    51121 gaacggtaaa ttagacggag ctgctcaacg tgttcctgta ccaacaggtt cattaacaga
    51181 attagtaaca gttcttgaaa aagaagtaac tgttgacgaa atcaatgcag caatgaaaga
    51241 agcttcaaac gaatcttacg gatacaacac agacgaaatc gtttcttcag atatcgttgg
    51301 tatgacttac ggttcattat ttgatgctac acaaactaaa gtaatgacag ttggcgacaa
    51361 acaattagtt aaaactgttg cttggtacga caacgaaatg tcatacactg cacaattagt
    51421 acgtacttta gaatacttcg ctaacttata agatagacaa acgtacaagt gtaaattata
    51481 gacatgtaca tttaaaagcg gggaagcaat cgcgcttctt ccgctttttt tgataataag
    51541 tttattcagg aggaaataaa atggctaaga aaacagtgaa agatatcgat ttaaaagaca
    51601 aaaaagttct tgtccgtgtt gacttcaacg ttcctttgaa agacggcgtg atcactgacg
    51661 acactcgtat caaagcagca ttgccaacaa tcaactacgt tctagaacaa ggcggaaaag
    51721 caatcctttt ctctcacctt ggacgtgtaa aaacagaaga agataaagaa ggaaaatctt
    51781 tagctccagt tgcaaaacgt ctaggcgaat tattaggtaa agaagtaaca ttcgtacctg
    51841 aaactcgcgg agaacaatta gaagaagcaa tccgcaacat gaatgacggc gacgttgtcg
    51901 tattcgaaaa tacacgattt gaagatattg acggtaaaaa agaaagcaaa aatgatacag
    51961 aactaggtaa atactgggct tcattaggcg atgtatttgt taacgatgct ttcggtacag
    52021 ctcaccgtgc acatgcttct aacgtaggta tcgcttcaac tggtatccca acagttgctg
    52081 gattcttgat ggaaaaagaa atcaagttca tcggtgaagc agtagaagaa ccaaaacgac
    52141 caatgattgc aatccttggt ggcgcaaaag tttctgataa aatcggcgta atcgaaaact
    52201 tgattcctaa agcagataaa atcttgatcg gtggcggaat gacttacaca ttctacgaag
    52261 caaaaggaat caagatcggt aactctctag tagaagctga taaagtggaa ctagcaaaag
    52321 aattaatcga aaaagcaggc gacaaacttg tcttgccaat cgacaatgtc tgtgcaccag
    52381 aattttcaaa tgatgttgaa acacaagtaa tcgaaggaga cattcctgat ggtttgatgg
    52441 ctttagacat cggcccagca tcagtaaaat tatttgctga tacattacaa ggtgcaaaaa
    52501 cagttgtttg gaacggacct atgggtgtat ttgaaatgag taactttgct aaaggtacaa
    52561 tcggtgtttg tgaagcaatc gctaacttag aaggcgcaac aacaatcatc ggtggcggag
    52621 actcagccgc agcagcagaa caattaggat ttgctgacaa attcacacac atctcaacag
    52681 gtggcggcgc aagcttggaa ttgcttgaag gtaaagaatt accaggacta gcagcaatca
    52741 acgataaata aacgaccaag tttgctcaat tctgacgagt aatcgaacaa aaggtgaagg
    52801 gagttttcat gactccctag ccatttttca ataaattaag gagtgtgttc ctcaatgcgt
    52861 aaacctatta tcgcaggtaa ctggaaaatg aacaaaactg catcagaagc aaaagaattt
    52921 gctgaagcag taaaaaacaa aatcccttca aatgacaaag ttgattcagt catcggatct
    52981 cctgcattat tcttacaaga attaatgaca tcagctgaag gtacagaact aaaaatctca
    53041 gcacaaaact gctactggga aaactcaggt gcgttcactg gtgaaacttc accagcagct
    53101 cttgctgatc taggtgttca ttatgtgatc atcggacatt cagaacgtcg tgaatatttc
    53161 catgaaacag acgaagacat caataaaaaa gcgaaagcta tctttgcaaa tggtatgaca
    53221 ccaatcctat gctgcggcga atctttagaa acctatgaag caggcaaaac tgctgaatgg
    53281 atcgaaggac aaattacagc tggtttagct gatctttcag ctgaacaggt gtcaaacatg
    53341 gttattgctt atgaaccaat ctgggcaatc ggtactggta aatcagctga cgcacaaatc
    53401 gctgacgaaa tttgtggagt tgtacgtcaa acagttgaaa aactttatgg aaaagaagta
    53461 tcagaagcag ttcgtatcca atacggcggt tctgtaaaac cagaaaatat tgctgaatac
    53521 atggcaaaag aaaatgttga cggtgcttta gtcggtggcg caagcttgca agcagattca
    53581 ttcttgtcat tgttagacgc tgtaaaataa ttgttgaaaa tttgcgaaaa atgcaagaaa
    53641 tgtttgcact aatgagcaaa attaactaaa atcgtatatg agggaaatcc cttaggaaaa
    53701 aacaaactca aaggagagac aaaacatgtc aattattact gatgtttacg ctcgcgagat
    53761 cttagactca cgtggtaacc caacaatcga agtagaagta tacacagaaa gcggagcttt
    53821 tggccgcgga atggttcctt caggtgcttc aactggtgaa tacgaagcag ttgaattacg
    53881 tgacggagac aaagctcgtt atggcggtaa aggtgtaact aaagctgtcg acaacgtaaa
    53941 caacatcatt gctgaagcaa tcattggcta cgatgttcgt gaccaaatgg ctatcgacaa
    54001 agctatgatc gctttagacg gaactcctaa caaaggtaaa ttaggtgcga acgctatttt
    54061 aggtgtttct atcgctgttg cacgtgcagc tgctgactac ctagaagtac ctttatacca
    54121 ctacctaggc ggattcaaca caaaagtatt gccaacacca atgatgaaca tcatcaacgg
    54181 cggatcacat gctgataact caatcgactt ccaagaattc atgatcatgc ctgttggcgc
    54241 tccaacattc aaagaagctc tacgtatggg tgctgaagta ttccacgcat tagcttcaat
    54301 cttgaaagct cgcggattag caacttctgt tggtgacgaa ggtggatttg ctccaaacct
    54361 tggttcaaac gaagaaggtt ttgaagtaat catcgaagca atcgaaaaag ctggctatgt
    54421 acctggtaaa gacgttgttc ttgctatgga tgctgcttct tcagaattct acgacaaaga
    54481 aaaaggtgtt tacgtactag ctgactcagg cgaaggcgaa aaaacaactg acgaaatgat
    54541 caaattctac gaagaattag tttctaaata tccaatcatc tcaatcgaag atggattaga
    54601 cgaaaacgac tgggacggat tcaagaaatt aactgacgta ttaggcgaca aagttcaatt
    54661 agttggtgac gacttgttcg taacaaacac tcaaaaatta tctgaaggta tcgaaaaagg
    54721 aattgctaac tcaatcctta tcaaagtgaa ccaaatcggt acattaacag aaactttcga
    54781 agctatcgaa atggctaaag aagctggcta cacagcagtt gtatctcacc gttctggtga
    54841 aacagaagat tcaacaatct ctgatatcgc tgttgcaaca aacgctggcc aaatcaaaac
    54901 tggttctcta tcacgtactg accgtattgc taaatacaac caattactac gtatcgaaga
    54961 ccaacttggt gaagttgcag aatacaaagg tttgaaatct ttctacaact taaaaaacaa
    55021 ataattcttt tctaatgaag atacatgatc gactattgaa agatagtcga gtatgatcac
    55081 ttagttaagt aatcgaactc tctacttgct gaaatatcag caggtagaga gtttttttgc
    55141 aactagcttc tgtatatgat gtttacaaag aatggtacaa gaaaagatca tccttgaaaa
    55201 aggaaagcta acaataacta gcatcatata agagctttta aggatagaac agtagaatag
    55261 gaatctatgg gcaagtcctc tcgtataaat aaaataggag ggaagtagca gcatattttt
    55321 cataaagtgg ctctctttct tcatttttac tttggttgct cctatttgaa aattcgagca
    55381 cactcaaata caataaaaag aaatggcaag ggagaggatt agatgaaaaa agaagaaaaa
    55441 atcgcgattt tacaagagat tatccgaatt aaatcagtga atggaaacga aggtgaagta
    55501 gcagcctatt tgaataagct tttggcaaag catgacataa caggagagat tgtttcttac
    55561 agagatggta gagataattt gattgctcgt taccaaaagg gccaatcagg aaaagtgtta
    55621 ggcctatctg gacatatgga tgtcgtggcg gctggtgatg aatcgtcttg gacctatgca
    55681 ccctttgcag cagaaatcca tgggaaccgt ttgtacggac gtggtgctac agatatgaag
    55741 agtggcttgg cagcaatggt gattgccatg atcgaactga aagagtcagg aaaaccattt
    55801 aacggaacag tcaaattatt agccactgtc ggagaagaag tgggtgaact aggtggggaa
    55861 cagctcacga aagcaggcta tgtagatgac ttagatgcct tgatcatcgg agagccaacc
    55921 aattacagtt tgatgtatac gcacatggga tcaatcaact atacagtcac ctcgcacgga
    55981 aaagaagcgc atagctctat gccagaccaa ggttataatg caatcaatca cctaaatgaa
    56041 ttcattacga aagcaaatgc tgaaatgaat cacctcgctg aaacaatcga gaatcctgtc
    56101 ctaggaaaaa caatccataa tgttacattg atcagtggcg ggaatcaagt caacagcatc
    56161 ccaagccatg cgcagctcca agggaacatt cgatcgattc cagaataccc aaatgataaa
    56221 atcattgcat tgctgcaatc aatcgtcaat gaattgaatc aagagacaga ctatcatttg
    56281 gaattgatga tcgattacaa caaaatacct gtcaaagctg atccagattc tcctttgatt
    56341 cattgcattc agcaacaatt cagccagcca ttacctttag ttggagcggc agcaacaacg
    56401 gatgcagccg aatttacgaa agctaaccat tccttcgact ttgtcgtctt tgggcctggt
    56461 gtcgttactt tgcctcatca agtagatgag tatgttgaaa tcgataatta tttggatatg
    56521 atcgaaaaat atcaaggaat tattttatcc tacttagcgt aagatttttt taat
//
LOCUS       DACEEZ010000008        56523 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20313_2017
            SAMN12639966-rid9879223.denovo.008, whole genome shotgun sequence.
ACCESSION   DACEEZ010000008 DACEEZ010000000
VERSION     DACEEZ010000008.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639966
            Sequence Read Archive: SRR10023158
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 56523)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 56523)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741683.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741683.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:44:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,016
            CDSs (total)                      :: 2,962
            Genes (coding)                    :: 2,889
            CDSs (with protein)               :: 2,889
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 36 of 73
            Pseudo Genes (incomplete)         :: 29 of 73
            Pseudo Genes (internal stop)      :: 18 of 73
            Pseudo Genes (multiple problems)  :: 8 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..56523
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639966-rid9879223.denovo.008"
                     /isolate="E20313_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(79..765)
                     /gene="gpmA"
                     /locus_tag="IWO89_000543"
     CDS             complement(79..765)
                     /gene="gpmA"
                     /locus_tag="IWO89_000543"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diphosphoglycerate-dependent
                     phosphoglycerate mutase"
                     /protein_id="HAQ0455324.1"
                     /translation="MPKLVFSRHGLSEWNALNQFTGWADVNLAPEGIEEAKEGGRKIK
                     EAGIEFDVAYTSVLTRAIKTCNLILEYSDQLWVPQIKSWRLNERHYGKLQGLNKKETA
                     EKYGDEQVHIWRRSYDTLPPLMEETDEGSAANDRRYAMLDKRDIPGGENLKVTLERAL
                     PFWQDEIAPALLDNKTVLVAAHGNSLRALAKHIEGISDEDIMDLEIPTGQPLVYELND
                     DLTVAKKYYL"
     gene            complement(937..1518)
                     /locus_tag="IWO89_000544"
     CDS             complement(937..1518)
                     /locus_tag="IWO89_000544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HAQ0455325.1"
                     /translation="MKIIGYARTTINDVDLTTQVDQLSDFGCQEIFHETYEVEQNDST
                     AVHLESVIADMQQGDALVICQLHRLGKSTRQLTELTQLFKDRGLHLISLDEGIDTREM
                     GKIYFQLMENLANMECALIKERTLVGLDEARKKGKIGGRPKIDAKTIKKIRRLYYEKK
                     ETIQFISSKCGVSVGTCYKYINLPEKEIEQLCI"
     gene            complement(1651..2334)
                     /gene="rpiA"
                     /locus_tag="IWO89_000545"
     CDS             complement(1651..2334)
                     /gene="rpiA"
                     /locus_tag="IWO89_000545"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-5-phosphate isomerase RpiA"
                     /protein_id="HAQ0455326.1"
                     /translation="MNLKQLAGIEAAKFVKDGMIVGLGTGSTAYYMVEEIGRRMREEG
                     LRITGVTTSNATKEQAEKLGIPLKSIDEVPVVDLTIDGADEISADFQGIKGGGAALLF
                     EKIVATYSKETIWIVDSSKLVDKLGKFPLPVEVIPYGSQQLLHIFEEKRFQPVLRTDE
                     NGEVLTTDGGHYIIDLHLEVIEQPESLATYLDELVGVVEHGLFLNMVSKVVVGSEKGV
                     EILDAIRRK"
     gene            2653..3066
                     /gene="rpsL"
                     /locus_tag="IWO89_000546"
     CDS             2653..3066
                     /gene="rpsL"
                     /locus_tag="IWO89_000546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007085295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HAQ0455327.1"
                     /translation="MPTINQLVRKPRKSKVEKSNSPALNKGYNSFKKTQTNVNSPQKR
                     GVATRVGTMTPKKPNSALRKYARVRLSNLIEVTAYIPGIGHNLQEHSVVLLRGGRVKD
                     LPGVRYHIVRGALDTAGVNDRKQSRSKYGTKKPKA"
     gene            3150..3620
                     /gene="rpsG"
                     /locus_tag="IWO89_000547"
     CDS             3150..3620
                     /gene="rpsG"
                     /locus_tag="IWO89_000547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HAQ0455328.1"
                     /translation="MPRKGPVAKRDVLPDPIYNSKLVTRLINRVMVDGKRGIAANIIY
                     NAFDLIKESTGNDPLEVFEQAMKNVQPVLEVKARRVGGSNYQVPVEVRPERRVTLGLR
                     WVVNYARLRGEHTMEERLAKEIMDAANNTGASVKKREDTHKMADANRAFAHYRW"
     gene            3714..5801
                     /gene="fusA"
                     /locus_tag="IWO89_000548"
     CDS             3714..5801
                     /gene="fusA"
                     /locus_tag="IWO89_000548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HAQ0455329.1"
                     /translation="MTREFSLQNTRNIGIMAHIDAGKTTATERILYYTGKIHKIGETH
                     EGASQMDWMEQEQERGITITSAATTAQWKGHRINIIDTPGHVDFTVEVERSLRVLDGA
                     VTVLDAQSGVEPQTETVWRQATTYGVPRIVFANKMDKIGADFLYSVSTLHDRLQANAH
                     PIQLPIGAEDDFTGIIDLVTMKAEMYTNDLGTEIEETEIPEEYRELAEEWREKLVEAV
                     AETDEELTLKYLEGEEITEAELKEGIRRATVNVEFYPVLCGSAFKNKGVQLLLDAVLD
                     YLPSPLDIPAIKGIDPKTDEEVERPADDSAPFSALAFKVMTDPFVGRLTFFRVYSGVL
                     QSGSYVQNATKGKRERVGRILQMHANSRSEISEVYAGDIAAAVGLKDTTTGDTLCDEK
                     SLVILESMEFPEPVIQVAIEPKSKADQDKMGVALQKLSEEDPTFRAETNVETGETIIA
                     GMGELHLDIIVDRMRREFKVEANVGAPQVSYRETFRAGTQAEGKFVRQSGGKGQYGHV
                     WIEFTPNEEGAGFEFENAIVGGVVPREYIPAVETGLKDAMENGVLAGYPLVDIKAKLY
                     DGSYHDVDSNETAFRVAASMALRAAAKKANPVILEPIMAVEVVIPEDYLGDVMGHVTA
                     RRGRVEGMEARAGGQQVVRAMVPLAEMFGYATTLRSATQGRGTFTMTFDHYEDVPKSV
                     QEEIIKKNGGKAE"
     gene            5973..7160
                     /gene="tuf"
                     /locus_tag="IWO89_000549"
     CDS             5973..7160
                     /gene="tuf"
                     /locus_tag="IWO89_000549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HAQ0455330.1"
                     /translation="MAKEKFDRSKPHVNIGTIGHVDHGKTTLTAAITTVLSKKNGGQA
                     MAYDQIDGAPEERERGITISTAHVEYETDTRHYAHVDCPGHADYVKNMITGAAQMDGA
                     ILVVSAADGPMPQTREHILLSRQVGVPYIVVFLNKVDMVDDEELLELVEMEVRDLLTE
                     YEFPGDDVPVVAGSALKALEGDASYEEKILELMAAVDEYIPTPERDNDKPFMMPVEDV
                     FSITGRGTVATGRVERGQVRVGDEVEVVGIAEETSKTTVTGVEMFRKLLDYAEAGDNI
                     GALLRGVAREDIQRGQVLAKPGTITPHTKFSAEVYVLTKEEGGRHTPFFTNYRPQFYF
                     RTTDVTGVVELPEGTEMVMPGDNVTMEVELIHPIAIENGTKFSIREGGRTVGAGVVTE
                     IKA"
     gene            7567..7875
                     /gene="rpsJ"
                     /locus_tag="IWO89_000550"
     CDS             7567..7875
                     /gene="rpsJ"
                     /locus_tag="IWO89_000550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003046044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S10"
                     /protein_id="HAQ0455331.1"
                     /translation="MAKQKIRIRLKAYEHRILDQSADKIVETAKRTGASVSGPIPLPT
                     ERSLYTIIRATHKYKDSREQFEMRTHKRLIDIVNPTPKTVDALMKLDLPSGVNIEIKL
                     "
     gene            7906..8535
                     /gene="rplC"
                     /locus_tag="IWO89_000551"
     CDS             7906..8535
                     /gene="rplC"
                     /locus_tag="IWO89_000551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3"
                     /protein_id="HAQ0455332.1"
                     /translation="MTKGILGKKVGMTQIFTESGELIPVTVVEATPNVVLQVKTMETD
                     GYEAIQVGYQDMREVLSNKPAKGHVAKANTAPKRFIREFKNVELGEYEVGKEITVDVF
                     QAGDIIDVTGTTKGKGFQGVIKRHGQSRGPMAHGSRYHRRPGSMGPVAPNRVFKNKRL
                     AGRMGGNRVTIQNLEVVRVDAERNVILIKGNVPGAKKSLITIKSAVKAK"
     gene            8563..9186
                     /gene="rplD"
                     /locus_tag="IWO89_000552"
     CDS             8563..9186
                     /gene="rplD"
                     /locus_tag="IWO89_000552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L4"
                     /protein_id="HAQ0455333.1"
                     /translation="MPNVALFKQDGSQNGEITLNEEIFGIEPNESVVYDAIVMQRASL
                     RQGTHAVKNRSAVRGGGRKPWRQKGTGRARQGSIRSPQWRGGGVVFGPTPRSYSYKLP
                     KKVRRLAMKSVLSEKVAENNLVAIEGLNFDAPKTKEFKQVLANLSIDSKVLVVLEKGN
                     DFAALSARNLPNVSVVTSDNVSVLDVVSNTKVLATQTALTQIEEVLA"
     gene            9186..9476
                     /gene="rplW"
                     /locus_tag="IWO89_000553"
     CDS             9186..9476
                     /gene="rplW"
                     /locus_tag="IWO89_000553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L23"
                     /protein_id="HAQ0455334.1"
                     /translation="MNLLDVIKRPVITEKSMLAMDDKKYTFEVDTRANKTLVKQAVEA
                     AFDVKVKNVNIVNVRAKFKRMGKYAGYTKKRRKAIVTLTEDSKEIQLFEAAE"
     gene            9515..10348
                     /gene="rplB"
                     /locus_tag="IWO89_000554"
     CDS             9515..10348
                     /gene="rplB"
                     /locus_tag="IWO89_000554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010747214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HAQ0455335.1"
                     /translation="MAIKKYKPTSNGRRNMTGSDFAEITTSTPEKSLLQPLKNHAGRN
                     NNGRITVRHQGGGHKRQYRVIDFKRNKDNVVATVQTIEYDPNRSANIALVHYEDGVKA
                     YILAPKGLEVGMKLVSGPEADIKVGNALPLENIPVGTVIHNIEMKPGKGGQLIRSAGT
                     SAQVLGKEGKYVLIRLNSGEVRMILAACRATIGSVGNEQHELINIGKAGRSRWMRKRP
                     TVRGSVMNPNDHPHGGGEGKAPIGRKAPVSPWGQPALGYKTRNKKAKSDKLIVRRRKN
                     K"
     gene            10391..10669
                     /gene="rpsS"
                     /locus_tag="IWO89_000555"
     CDS             10391..10669
                     /gene="rpsS"
                     /locus_tag="IWO89_000555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000533765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19"
                     /protein_id="HAQ0455336.1"
                     /translation="MGRSLKKGPFVDEHLMKKVEAQAGVEKKKVIKTWSRRSTVFPSF
                     VGYTIAVYDGRKHVPVYIQEDMVGHKLGEFAPTRTYRGHAADDKKTKR"
     gene            10692..11039
                     /gene="rplV"
                     /locus_tag="IWO89_000556"
     CDS             10692..11039
                     /gene="rplV"
                     /locus_tag="IWO89_000556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007207624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L22"
                     /protein_id="HAQ0455337.1"
                     /translation="MAEQITSAKATAKTVRTSPRKARLVIDLIRGKSVADAISILKFT
                     PNKSAGIIEKVLMSAVANAENNFDLDVENLVVSEAFVNEGPTMKRFRPRAKGSASPIN
                     KRTSHITVVVSEK"
     gene            11053..11709
                     /gene="rpsC"
                     /locus_tag="IWO89_000557"
     CDS             11053..11709
                     /gene="rpsC"
                     /locus_tag="IWO89_000557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000529931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3"
                     /protein_id="HAQ0455338.1"
                     /translation="MGQKVHPIGMRVGIIRDWDAKWYAEKEYAEFLHEDLRIRKFIST
                     KLADAAVSTVEIERATGRVNISIHTAKPGMVIGKGGSEVESLRKELNKLTGKRIHINI
                     VEIKKPDLDAKLVGEGIARQLENRVAFRRAQKQAIQRSMRAGAQGIKTQVSGRLNGAD
                     IARSEGYSEGTVPLHTLRADIDYAWEEADTTYGKLGVKVWIYRGEILPTKKNTEKGGK
                     "
     gene            11712..12146
                     /gene="rplP"
                     /locus_tag="IWO89_000558"
     CDS             11712..12146
                     /gene="rplP"
                     /locus_tag="IWO89_000558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L16"
                     /protein_id="HAQ0455339.1"
                     /translation="MLVPKRVKHRREFRGKMRGEAKGGKEVAFGEYGLQAVDSHWITN
                     RQIEAARIAMTRYMKRGGKVWIKIFPHKSYTAKAIGVRMGSGKGAPEGWVAPVKRGKI
                     MFEIAGVPEEVAREALRLASHKLPMKTKIVKREEMGGESNEG"
     gene            12136..12324
                     /gene="rpmC"
                     /locus_tag="IWO89_000559"
     CDS             12136..12324
                     /gene="rpmC"
                     /locus_tag="IWO89_000559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L29"
                     /protein_id="HAQ0455340.1"
                     /translation="MKVKEIRELTTAEMLDQEKQLKEELFNLRFQLATGQLENTARIK
                     EVRKSIARIKTVLREQAK"
     gene            12349..12615
                     /gene="rpsQ"
                     /locus_tag="IWO89_000560"
     CDS             12349..12615
                     /gene="rpsQ"
                     /locus_tag="IWO89_000560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016184196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17"
                     /protein_id="HAQ0455341.1"
                     /translation="MTEGRNQRKVYQGRVVSDKMDKTITVAVETKKNHPIYGKRMNYS
                     KKYKVHDENNTAKVGDIVRIMETRPLSATKRFRLLEVVEEAVII"
     gene            12673..13041
                     /gene="rplN"
                     /locus_tag="IWO89_000561"
     CDS             12673..13041
                     /gene="rplN"
                     /locus_tag="IWO89_000561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002460061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HAQ0455342.1"
                     /translation="MIQAESRLKVADNSGAREILTIKVLGGSGRKTANIGDVIVATVK
                     QATPGGVVKKGDVVKAVIVRTKSGARRTDGSYIKFDENAAVIIRDDKSPRGTRIFGPV
                     ARELRENNFMKIVSLAPEVL"
     gene            13078..13386
                     /gene="rplX"
                     /locus_tag="IWO89_000562"
     CDS             13078..13386
                     /gene="rplX"
                     /locus_tag="IWO89_000562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HAQ0455343.1"
                     /translation="MFVKKGDKVKVITGKDKNKEGVVLAAFPKKDKVIVEGVNVVKKH
                     QKPSQAAPQGGIVEMEAPIHVSNVMVVDSTGVAGRVGYKEVDGKKVRVSKKTGEVLDK
                     "
     gene            13414..13953
                     /gene="rplE"
                     /locus_tag="IWO89_000563"
     CDS             13414..13953
                     /gene="rplE"
                     /locus_tag="IWO89_000563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016185989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HAQ0455344.1"
                     /translation="MNRLKEKYLKEVTPSLMEKFNYSSVMQTPKVEKIVINMGVGDAV
                     SNAKNLDKAVEELTLITGQKPMITKAKKSIAGFRLREGMPIGAKVTLRGERMYEFLDK
                     LVSVSLPRVRDFHGVSKKAFDGRGNYTLGIKEQLIFPEVDYDLVDKVRGMDIVIVTTA
                     NSDEESRELLTQLGMPFQK"
     gene            13972..14157
                     /locus_tag="IWO89_000564"
     CDS             13972..14157
                     /locus_tag="IWO89_000564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011245027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type Z 30S ribosomal protein S14"
                     /protein_id="HAQ0455345.1"
                     /translation="MAKKSMIAKNKRPAKFSTQAYTRCERCGRPHSVYRKFHLCRICF
                     RELAYKGQIPGVKKASW"
     gene            14193..14591
                     /gene="rpsH"
                     /locus_tag="IWO89_000565"
     CDS             14193..14591
                     /gene="rpsH"
                     /locus_tag="IWO89_000565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010742763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HAQ0455346.1"
                     /translation="MVMTDPIADFLTRIRNANMVKHESLEVPASKIKRDIAEILKREG
                     FVRDVEYIEDDKQGVIRVFLKFGKNGERVITNLKRISKPGLRAYVKADEVPKVLNGLG
                     IAIISTSEGVVTDKEAREKNIGGEVIAYVW"
     gene            14623..15159
                     /gene="rplF"
                     /locus_tag="IWO89_000566"
     CDS             14623..15159
                     /gene="rplF"
                     /locus_tag="IWO89_000566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HAQ0455347.1"
                     /translation="MSRIGNKVVVIPEGVTVTQDGNNVTVKGPKGELTRTFSADIKMN
                     IEGNEVTFTRPNDSKEMKTIHGTTRANFNNMVVGVSEGFQKALELIGVGYRAQLQGSK
                     LVLNVGYSHPVEITPPAGVTVEVPANTQVIVKGANKEEVGELAANIRGTRPPEPYKGK
                     GIRYVGEYVRRKEGKTGK"
     gene            15320..15676
                     /gene="rplR"
                     /locus_tag="IWO89_000567"
     CDS             15320..15676
                     /gene="rplR"
                     /locus_tag="IWO89_000567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014125626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HAQ0455348.1"
                     /translation="MITKPDKNKTRQKRHRRVRNNISGTAERPRLNVFRSNKNIYAQI
                     IDDVAGVTLASASTLDKEISGGTKTEAAQAVGKLVAERAAEKGIKVVVFDRGGYLYHG
                     RVQALAEAARENGLEF"
     gene            15697..16197
                     /gene="rpsE"
                     /locus_tag="IWO89_000568"
     CDS             15697..16197
                     /gene="rpsE"
                     /locus_tag="IWO89_000568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HAQ0455349.1"
                     /translation="MVYIDPKHLELEDRVVAINRVTKVVKGGRRLRFAALVVVGDKNG
                     HVGFGTGKAQEVPEAIRKAIEDAKKNLVEVPMVGSTIPHEVIGAFSGGRILMKPAVEG
                     SGVAAGGPVRAVLELAGVADITSKSLGSNTPINVVRATVEGLKQLKRAEEVAELRGKS
                     VEELIG"
     gene            16212..16391
                     /gene="rpmD"
                     /locus_tag="IWO89_000569"
     CDS             16212..16391
                     /gene="rpmD"
                     /locus_tag="IWO89_000569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L30"
                     /protein_id="HAQ0455350.1"
                     /translation="MAELKVTLKRSIIGRPQNQRDTVKALGLTKINSSVVKPSNEAIK
                     GMINTVSHLVDVEEI"
     gene            16435..16875
                     /gene="rplO"
                     /locus_tag="IWO89_000570"
     CDS             16435..16875
                     /gene="rplO"
                     /locus_tag="IWO89_000570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15"
                     /protein_id="HAQ0455351.1"
                     /translation="MKLHELKPAEGSRQVRNRVGRGTSSGNGKTAGRGQKGQKARSGG
                     GVRLGFEGGQTPLFRRLPKRGFTNVNRKDYAVINLDVLNRFEDGAEVTPVALVEAGIV
                     KNEKAGIKVLANGELNKKLTVKAAKFSKAAQEAIEAAGGSIEVI"
     gene            16875..18170
                     /gene="secY"
                     /locus_tag="IWO89_000571"
     CDS             16875..18170
                     /gene="secY"
                     /locus_tag="IWO89_000571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HAQ0455352.1"
                     /translation="MFRLLKDAFKVKDIRSKILFTVFVLFVFRLGAHITVPGVNAASL
                     QNLSSLPFFNMLNLVSGSAMQNFSIFSMGVSPYITASIVIQLLQMDIVPRFVEWSKQG
                     EVGRKKLNQATRYLTLVLAFIQSVGITAGFNQYAQLGFVNNPNVATYVTIGLILTAGT
                     MFVTWLGEQITEKGIGNGVSMIIFAGIISRLPESIREIYEDYFINIDPSDIWKSAIFV
                     AILLVAVLLIVTFVTFFQQAERKIPIQYTKRVAGAPTSSYLPLKVNAAGVIPVIFASS
                     FIATPNAVLQALSGRFGSENWYKVMMEIFNYNTAPGATIYTILIVAFTFFYAFVQVNP
                     EKLAENLQKQGSYIPSVRPGKGTEEYVSRLLMRLSTVGAVFLGLVALLPIIAQMVWDL
                     PQSIGLGGTSLLIVIGVALETAKQLEGLMLKRKYVGFIE"
     gene            18232..18879
                     /locus_tag="IWO89_000572"
     CDS             18232..18879
                     /locus_tag="IWO89_000572"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HAQ0455353.1"
                     /translation="MNLILMGLPGAGKGTQAEKIIDAYGIPHISTGDMFRAAMQNETA
                     LGLEAKSYMDKGALVPDEVTNGIVKERLAEPDTEKGFLLDGFPRTLDQAKALDAMLKD
                     LNKKIDAVIDIHVGEEILVERLAGRFICSNCGATYHKIFNPTKVEDTCDRCGGHEFYQ
                     REDDKPETVKNRLAINIKNSEPILAYYKEQGLLNTIDGAREIDAVFADVQKIIEK"
     gene            19070..19288
                     /gene="infA"
                     /locus_tag="IWO89_000573"
     CDS             19070..19288
                     /gene="infA"
                     /locus_tag="IWO89_000573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003720929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HAQ0455354.1"
                     /translation="MAKEDMIEVEGTVVETLPNAMFKVELENGHQVLATVSGKIRMHY
                     IRILPGDKVTVELSPYDLNRGRITYRFK"
     gene            19321..19437
                     /gene="rpmJ"
                     /locus_tag="IWO89_000574"
     CDS             19321..19437
                     /gene="rpmJ"
                     /locus_tag="IWO89_000574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013773089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L36"
                     /protein_id="HAQ0455355.1"
                     /translation="MKVRPSVKPMCEHCKVIRRKGRVMVICPANPKHKQRQG"
     gene            19455..19820
                     /gene="rpsM"
                     /locus_tag="IWO89_000575"
     CDS             19455..19820
                     /gene="rpsM"
                     /locus_tag="IWO89_000575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008809894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HAQ0455356.1"
                     /translation="MARIAGVDIPRDKRVVVSLTYIYGIGNTTAKKILADAGVSEDVR
                     VRELTNEQTDAIRAEVDKLKVEGDLRREVNLNIKRLMEIGSYRGIRHRRGLPVRGQNT
                     KNNARTRKGPARAIAGKKK"
     gene            19848..20237
                     /gene="rpsK"
                     /locus_tag="IWO89_000576"
     CDS             19848..20237
                     /gene="rpsK"
                     /locus_tag="IWO89_000576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016615824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HAQ0455357.1"
                     /translation="MAAKKVNRKRRVKKNIEAGIAHIHSTFNNTIIMITDTHGNALAW
                     SSAGSLGFKGSKKSTPFAAQMAAETATKAAMEHGLKTVEVTVKGPGSGREAAIRSLQA
                     TGLEVTAIRDVTPVPHNGARPPKRRRV"
     gene            20317..21255
                     /locus_tag="IWO89_000577"
     CDS             20317..21255
                     /locus_tag="IWO89_000577"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010774253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HAQ0455358.1"
                     /translation="MIEFEKPRIAKIDEEKDYGKFIVEPLERGYGTTLGNSLRRILLS
                     SLPGAAITNIQIDGVLHEFSTIKGVREDVTQIILNIKGLALKMYGQEEKTLEIDITGP
                     ATVTAGDIIVDSDVEILNKDMYICSVSEGATFHARLTVKPGRGYVQADENKKEDMPIG
                     VLPVDSIYTPVRRVNYQVENTRVGHREDFDKLTMEIWTDGSIEPLEAMSLSAKIMTEH
                     LDIFVNLTDEAKNAEIMVEKEETQKEKMLEMTIEELDLSVRSYNCLKRAGINTVQELT
                     NKSEPEMIKVRNLGRKSLEEVKAKLHDLGLGLRKED"
     gene            21283..21663
                     /gene="rplQ"
                     /locus_tag="IWO89_000578"
     CDS             21283..21663
                     /gene="rplQ"
                     /locus_tag="IWO89_000578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013773093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
                     /protein_id="HAQ0455359.1"
                     /translation="MSYRKLGRTSSQRKAMLRDLTTDLLINERIVTTEARAKEVRSTA
                     EKMITLGKRGDLHARRQAAAFVRNEVASVREENEDIVIESALQKLFNDIAPRYAERQG
                     GYTRILKTEPRRGDGAPMVVLELV"
     gene            21935..22774
                     /locus_tag="IWO89_000579"
     CDS             21935..22774
                     /locus_tag="IWO89_000579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter
                     ATP-binding protein"
                     /protein_id="HAQ0455360.1"
                     /translation="MEPIIELEKINYKYQPDDLRPALKDVSFTIDKGEWIAIIGHNGS
                     GKSTLAKTINGLLLPESGIVKVGNQILDEENIWTIRQMVGMIFQNPDNQFVGSTVEDD
                     VAFGLENQGIPREEMLVRVKDALEKVRMAEFASREPARLSGGQKQRVAIAGVVALRPD
                     IIILDEATSMLDPEGREEVISTIKKIKEESQLTVISITHDIDEAANANRILVMRQGEL
                     VREGTPKEIFSAGPELIDLGLDLPFPEKLKSALKERGVDVPSEYMTEERMVDWLWTSV
                     LNK"
     gene            22750..23619
                     /locus_tag="IWO89_000580"
     CDS             22750..23619
                     /locus_tag="IWO89_000580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter
                     ATP-binding protein"
                     /protein_id="HAQ0455361.1"
                     /translation="MDIRFEQVDFTYQPNTPFEQRALFDINMTIKENSYTALVGHTGS
                     GKSTLLQHLNALVKPTSGTVHIGERDIQPDTDNKNLKPIRKKVGIVFQFPEAQLFEET
                     VAKDIAFGPKNFGVSEEEALVLAKETLEQVGLDESYLERSPFELSGGQMRRVAIAGVL
                     AMRPEVLVLDEPTAGLDPQGRKEMMEMFWRLHKEQQITIVLVTHLMDDVANFADYVYV
                     LEKGRIVNSGEPQEVFQNIEWLKEKQLGVPTATEFAEELMAKGMNFATLPLTAEELAD
                     AIVHYAGGNAHDE"
     gene            23609..24406
                     /locus_tag="IWO89_000581"
     CDS             23609..24406
                     /locus_tag="IWO89_000581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     protein EcfT"
                     /protein_id="HAQ0455362.1"
                     /translation="MMNKLIFGRYMPGDSLIHRLDPRAKLLASFYFIGIIFLANNWET
                     YLLLAFFTLLSVFLSKVDLKFFIRGIRPLIWLILFTVALQVLFTSGGETYWSWGVFHI
                     TEFGVMNGLFIFCRFVLIIFMSTLLTLTTPPLELSDAIEYLLRPLKAVRFPVHEISLM
                     LSIALRFVPTLMDETEKIMNAQRARGVDFGEGNLVQKMKAVIPLLIPLFVSSFNRAED
                     LATAMEARGYKGGEGRTKYRVLHWHNRDTMVIIAFVLLTVLLIFLRG"
     gene            24609..25355
                     /gene="truA"
                     /locus_tag="IWO89_000582"
     CDS             24609..25355
                     /gene="truA"
                     /locus_tag="IWO89_000582"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HAQ0455363.1"
                     /translation="MVRYKAIIAYDGTNFNGFQKQPNGRTVQEEVEKTLQKMANGKEI
                     TVFGSGRTDAGVHAIGQVIHFDYPEERPLERMRFALDTQSPEDIAVRQVEIVSEDFHA
                     RYLVKEKTYQFRVDIGKPRSPFRRHYASYFPYPLDLEKIQRALPDLLGTHDFTSFCAS
                     GSSIEDKIRTIYEAKMEVNETKDELLFTFRGNGFLYKMIRILVGTLLKIGNGRLPEDS
                     IPDIIAKKDRNAAGPTAHPEGLYLYEVKYE"
     gene            complement(25688..28357)
                     /locus_tag="IWO89_000583"
     CDS             complement(25688..28357)
                     /locus_tag="IWO89_000583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfhO family protein"
                     /protein_id="HAQ0455364.1"
                     /translation="MKKNLKSFFRENGRYAAASFLIPFLILALVYLSIGIYPGSSRSI
                     LASDAFSQFSNFHASFRNVLLGKQSIFYTWNASLGLNYLSLISYYLGGIFTPLVFFFP
                     NQMMPDALYVLTLLKVGCAGLAFWFLARTFKIPRWGHVALSVSYALISFITAHSEIIM
                     WLDAFVYLPLVILGIHRVMDLKRPKLLFISYLLLFLSSFYMGFMIGVFSFLYFIARLL
                     TNWSEYKKTIVPYGITSLLAGGASMIIVLPAVLDLRSNGEALSQITTLKTEATSYLDL
                     IMKNMIGVYDTTKYGSIPFVYAGLLPLVLCLFYFASRKVPLKNKFLYGSLFAILIASF
                     YLVPLNLFWHGMHAPNMFLFRYAYLFSFLVLLLAGKGWEKIEQDDGGLLLGIILVVAA
                     LFAVAWGIKPEGSYTYVTMTSFVLTVVFLGIYAFAVGFYQHKKLSFNHLSILLLLIMS
                     AEALVNTNSMIHGILDDWNYASRSLYTDPYPDLKALVNETKKDSDSFYRLENLDPVSA
                     NDSINYGYSGISLFSSIRNRNSSSYLDKLGFRSRGTNLNIRYNNNTLLMDGFTGIKYN
                     ISKNESTFSKYGFRQAGQSGDYTLYQNVNALPLAFTAPLSVNDVEQPVTDNLTSQTNL
                     INTLSGLNEQYFTFYTPTLKNQLNTTITKTTTGVTYSETADNQPKELTWEVHVPANTQ
                     AYLSLFPTNFAQLESSSATITVNGTSRKTQINISGQYYDLGYYPQETTVTFTASFYGT
                     KEISFMEPKVVGLDVVAYQAAMNQIKEKGVEMEATGRKASGTVNTAEDSMLVTTIPYD
                     KGWKAKIDGKPVKVSNFKDAFLMIKVPAGKHTVTFSYLPEGFTIGAILFVLCIALFIL
                     YVWYLDRNRPFYGTIDPAIEEQPRNTHRRRRRK"
     gene            complement(28595..28783)
                     /locus_tag="IWO89_000584"
     CDS             complement(28595..28783)
                     /locus_tag="IWO89_000584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007207596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HAQ0455365.1"
                     /translation="MAKVCYFTGRKTKSGNNRSHAMNSTKRTVKPNLQKVRVMVDGKP
                     KKVWVSTRALKSGKVERV"
     gene            29053..29415
                     /locus_tag="IWO89_000585"
     CDS             29053..29415
                     /locus_tag="IWO89_000585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HAQ0455366.1"
                     /translation="MAVKIQTSAGTIEITNDVIATVVGGAATDVFGVVGMASKNQIKD
                     NITEILRKENYSRGVVVRQEDNGIAVDVYTIISYGTKISEVSRNVQEKVKYNLEALLG
                     VTANSVNVFVQGVRVLPD"
     gene            29467..31149
                     /locus_tag="IWO89_000586"
     CDS             29467..31149
                     /locus_tag="IWO89_000586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DAK2 domain-containing protein"
                     /protein_id="HAQ0455367.1"
                     /translation="MSVTEISASQFQEMVQAGANRLQVNAEYVNSLNVFPVPDGDTGT
                     NMNLSMTSGAKAVVDSTSEKVGELAAMLSKGLLMGARGNSGVILSQLFRGFSKQIPDV
                     VSLNASDLAAAFTHGVETAYKAVMKPVEGTILTVAREAAKAGEKKAKSTDDVVEVMTA
                     VVKGAKRALAKTPDLLPVLKEVGVVDSGGQGLLFVYEGFLSALNGEFQADETYEPSPA
                     EMDDMVNAEHHRSVQGQMATEDIHFGYCTEIMVKIGEGPTVDSTFDYEEFRNYLDGIG
                     DSLLVVNDDEIIKVHVHTENPGEVLNYGQKFGSLVKIKVDNMRLQHETILEHDEEVNE
                     FAKKEIPRIPYGIVAIAAGKGVQELFESLGANYVISGGQTMNPSTEDIVKAIQEVNAD
                     KVIVLPNNKNIFMAADQAAEVADIPVAVVPSRTISQGLTAMLSFNDQASLEENKETMT
                     EMLSSVVSGQVTHAIRDTSIDGVEIKEGDFLGMIDGKILISNPDITATALSTLEQMIN
                     EDTEIVTILAGDEGTVEQANEFAEKLTEKHPDLEIEIHQGDQPVYPYLFSAE"
     gene            31266..33302
                     /gene="recG"
                     /locus_tag="IWO89_000587"
     CDS             31266..33302
                     /gene="recG"
                     /locus_tag="IWO89_000587"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecG"
                     /protein_id="HAQ0455368.1"
                     /translation="MRSLDDPITMLAGVGEKRAASLASLGIQTIEDLLTYYPFRYEDI
                     QERNLNEIQDQEKVTLKGIVVSPPVMNRFGYKKSRLQFRMMQEHDVFNVSFFNQPYLK
                     EKVILSEEIAVYGKWDAKRKALNGMKILGSQTMDDFSPIYHVNKSIRQTTLVDLIKKG
                     FQEYGTLIEENLPNNLVEKYRLLDRASAVRSMHFPKDHEENHQAKRRVVFEEFFLFQM
                     RLQGLKKAEKAETNGLEILYDVQKLKQFTQKLPFELTNAQKRVTNEICRDLRSPQHMQ
                     RLLQGDVGSGKTVVAAIALYATVTAGFQGALMVPTEILAQQHMESLQQLFDPNEVTIA
                     LITGSTKAKERRELLEKLEQGEINVVIGTHALIQDGVEFQNLGLVITDEQHRFGVNQR
                     KVLREKGWRPDVLFMTATPIPRTLAITAYGEMDVSIIDELPAGRIPIETRWVRTPQLD
                     SVLDWTKKELARGHQMYVICPLIEESEALDVKNAVEIYEKLRELFAPKYEVGLLHGKM
                     KNQEKEVIMEAFKDNQMQILVSTTVIEVGVNVPNATVMLIMDADRFGLAQLHQLRGRV
                     GRGSDASYCILVANPKNELGVERMKIMTETTNGFVLSEKDLELRGPGEVFGFRQSGLP
                     QFAIADLVTDGNVLEVAREEAAALWQIKDWQMLPEYQPLAQSLQIENQDNRYFD"
     gene            33351..34352
                     /gene="plsX"
                     /locus_tag="IWO89_000588"
     CDS             33351..34352
                     /gene="plsX"
                     /locus_tag="IWO89_000588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase PlsX"
                     /protein_id="HAQ0455369.1"
                     /translation="MKIAVDAMGGDHAPQAIVEGVALAQQDFPEVEFLLYGKEAEIKK
                     YLTNEKNITIIHTDEKINSDDEPVKAIRRKKTASMVLAAQAVKNGEADAVFSAGNTGA
                     LLAAGLFIVGRIKNIERPGLMSTLPVVGKEGAGFDMLDLGANAENKPEHLLQYGILGS
                     FYARKVRGIGRPRVALLNNGTEATKGSEVTKKAYELLQNETSLNFIGNVEARELLNGV
                     ADVVVTDGFTGNAVLKSIEGTAMNMMNLLKSAILNEGIKGKMGAMLLKDGLRSLKAEM
                     DYSKHGGAVLFGLKAPVIKTHGATGPDAVRYTIRQIHTMLETDVVGQLVEQFEKSEE"
     gene            34474..34716
                     /gene="acpP"
                     /locus_tag="IWO89_000589"
     CDS             34474..34716
                     /gene="acpP"
                     /locus_tag="IWO89_000589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HAQ0455370.1"
                     /translation="MTREEVFNKVAKIISNHFEIDTDKVTDELNIKDDLKADSISIME
                     FVLELEDEFGTEISDEDAEQIETVGGAVDYISSHLK"
     gene            34984..35988
                     /locus_tag="IWO89_000590"
     CDS             34984..35988
                     /locus_tag="IWO89_000590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ0455371.1"
                     /translation="MSDNQLLEVQNLHTGFRLKDDYYDAVDDVSFTLDKNEILAIVGE
                     SGCGKSTLATTIMGLLDPNNTKITGEILYNDLNLIDLNETLYNKIRGNDIGMIFQDPL
                     SALNPLMRIEDQIKEGLSYHTKMNAEQRQARALELLEQVGIPNPARVGRQYPHELSGG
                     MRQRVIIAIAIACKPPIVIADEPTTALDVTIQAQILDLLKDLQEETKTGIILITHDLG
                     VVAEMADRVAVMYGGQFVEVAGVKELFDHPKHPYTRSLLNSIPQEGNHDAELHVIEGV
                     VPSLKNMPRKGCRFAPRIPWIPASDHEEEPILHEVAPNHLVRCSCYKHFHFRDEKGEV
                     "
     gene            35989..36933
                     /locus_tag="IWO89_000591"
     CDS             35989..36933
                     /locus_tag="IWO89_000591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ0455372.1"
                     /translation="MTELITIKDLKVHYPIRSGFFNRVTDHVYAVDGVDFIIEKGKTY
                     GLVGESGSGKSTTGKAVVGLEKVTAGQIMYEGKDVTKRSNRKKMGYNKDVQMIFQDSM
                     SSLNPKKRVLDIIAEPIRNFERLSDQEEKKKVKGLLDIVGMPEDALYKYPHEFSGGQR
                     QRLGVARAVATNPKLIVADEPVSALDLSVQAQVLNFMKRIQQEYGLSYLFISHDLGVV
                     KHMCDNIAIMYKGRFVEIGTREDIYNDPRHIYTKRLLSAIPQIDVENRESHKENRRRV
                     EQEYIQNQKEYYDATGRVYDLRTITTTHKVALKDGGAS"
     gene            36933..37895
                     /locus_tag="IWO89_000592"
     CDS             36933..37895
                     /locus_tag="IWO89_000592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAQ0455373.1"
                     /translation="MWKTILRRVLLMIPQVIILSVLIFLLAQAMPGDPFTGLINPNQD
                     PAVIEQMRQAAGLNDPWYVQYFRWIGNALQGDFGQSFLYKRSVADLIGERIINTLYLS
                     ILTVVLTYLIAVPLGMLAGRYQNSILDKIVVIYNFFSFAVPLFIFGLIMLFVFGYRLG
                     WFPTSGSQTLGLQGGFITQWIDRLKYILLPSITQAFLATAVTIQYLRSEVIDSKSLDF
                     VRTARSKGVPTNKVFSRHIFRNAALPMASQMGYEITSLIAGSVVIEKIFGYPGVGKLF
                     IDSIGQRDYTVITSLVLILGIATLVGTLLSDIIMSIVDPRIRIQ"
     gene            37922..38836
                     /locus_tag="IWO89_000593"
     CDS             37922..38836
                     /locus_tag="IWO89_000593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAQ0455374.1"
                     /translation="MSEKNNTAVQESIPPMGIRMIAREFKKDKIAIFSLGLLVVLLLV
                     IFIGAMLTDQDKVMYVSIFDKYAAPGTVSTQGQKFILGADEGGRDVLGQLIIGARNSV
                     TIGFAITILTSIIGVGLGIISGYYGGIIDNILMRIVDFIMILPIMLIIIVFVSIISRY
                     NVWSFIFIMSAFYWVAKARLFRSKTLSEARRDYVSASKTMGTNDFKIMFREIMPNLSS
                     LIITNLTMNFAANIGIETTLTFLGFGLPANVPSLGTLIGYASNGEVLVNRQWIWLPAS
                     ILILVLMLSINYVGQAFKRSADARQRLG"
     gene            38862..40643
                     /locus_tag="IWO89_000594"
     CDS             38862..40643
                     /locus_tag="IWO89_000594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HAQ0455375.1"
                     /translation="MKKTIFGFAALMSAVVLAACGGGGTDTSGNNSGGNGNNKEELTF
                     PIATKNNDDAIEDGELEAAVATDSQFKGLFQWEFYQDSYDAAFMAPSHEPLFANDDDF
                     RITDDGAATLDLDEDTKKATIKVKDNVKWSDGEDVTADDVIFPYEIIGNKDYTGIRYD
                     DTFRNIVGMEEYHDGSADTISGIKKIDDKTVEIEYKEVNPSMLQSGGGIWSYAAPKHT
                     LEGVAIKDMESSDQVRKNPVTFGPYYMSNIVAGESVEYLPNEHYWNGTPKLKKITMKS
                     VPMASATEALNSKLYDMIFQMPTDTYDTYKEIAGYTNLGREQTSYTYLGFKLGKWNAD
                     TKSVEYNPDSKMADKSLRQAMGYALDNNAVGERFYAGLRSNATSLIPPVFQSYHDDEM
                     KGFTQDLDKAKKLLDDAGYKDVDDDGLREDKDGNKLTINFASMSGGETAQPLADYYIQ
                     QWKEIGLDVQYTTGRLIEFNSFYDKLENDDPEIDVYQAAWNTGTDPNPSGLWGPNAAF
                     NYTRYESEENTKLINDISSSKAFDEEYQKEAYQKWQQYAFEEAFAIPTLFRNEVLPVN
                     DRVKDWDWSYDVPNPWANVSVTADSRS"
     gene            40991..41677
                     /locus_tag="IWO89_000595"
     CDS             40991..41677
                     /locus_tag="IWO89_000595"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HAQ0455376.1"
                     /translation="MDNQLTKELKENYNIVFHDLNLLEQAFTHSSYVNEHRNLQLSDN
                     ERLEFLGDAVLELMVSQYLFRLYPHLPEGKLTKTRAAIVREDSLSKFAKECHFDQYVL
                     LGKGEENSGGRTRPALLCDLFEAFLGALYLDQGFDTTHAFIEKVIFPKVKAGAFSHEM
                     DHKTKLQEVLQKSGDVSIEYRLINEEGPAHERVFWIEVYVDDQLIGTGQGKSKKLAEQ
                     AAAENALAAL"
     gene            41697..45278
                     /gene="smc"
                     /locus_tag="IWO89_000596"
     CDS             41697..45278
                     /gene="smc"
                     /locus_tag="IWO89_000596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome segregation protein SMC"
                     /protein_id="HAQ0455377.1"
                     /translation="MYLKRIEIAGFKSFADKTVIDFENSVTAVVGPNGSGKSNITEAI
                     RWVLGEQSAKSLRGGKMPDIIFAGSDTRKQLNIAEVTVILDNSDHYLPLEYNEISVTR
                     RYRRTGESEFFINKQSCRLKDIQELFLDSGLGKESFSIISQGKVEAIFSSKPEDRRGI
                     FEEAAGVLKYKQRKKKAEQKLFETEDNLSRVQDIIHELEEQLTPLAAQSEAAKEFLRL
                     KETLTQTDVSLMVAEIKTAKKDWDNKQAQLAKFNLELGKLSESIQEQESILAKQRKEN
                     AQADRLIEKNQQVLLDLSEKLKQTEGQKDVLQERTKHTQKSSQEYQTSLAEAQKKVKH
                     FEKLQESLMKAAAEKETEIQKAEANLIKTQQELEKYQKSTKELLAELRDQYVDLMQEQ
                     AAVGNELKYLERQYIQETAKSKQTLAKQSEVEASVDRLILQKEELTQKQAQLKSSLTE
                     TKEKLEMIQQNGKKFQEALAKEQPKMYQLMNQVQQLRARKKSLQEIQENYFGFYQGVR
                     LVLQHKQQLSGIVGAVAELIDVPADFTLAIETALGGAAQHVIVENEKDARQAITYLKQ
                     QRGGRATFLPLTTIKPRQLPAHILTQAAAVEGFIGIASEQVSYPDQIQTVVQNLLGTI
                     LLAKDLTSANAIAQTIRYQYRVVSLEGDVMNAGGSMTGGANKRGNQGSLFVQNQELKQ
                     LTSEFEEADKQLQAQEKKVQELQQETARLAEEQEVLRTRGEQLRFEEQEATNQLQNII
                     NELERFEKEKQISTFETRELQQFIEDYEKQTNELKDKQTDLESQRQKIDEEIKSLSQE
                     SDQMEARRAQVQSQKAQEQADLAVLKEQFNHLQIQLRGARVQKAEATERQEAIEKQLA
                     TLTADFSDHEVTEESLEKQINELSAQRETLKAELAKAKEQRDRRQKEIDKLEAVLAER
                     NREQKARLSEQSKLEVQKDRAEMILDNHLTYLQSEYQLTFEKASQDYQETTDIEDSRT
                     KVSSLKEQIEKLGPVNLNSIEQYEQVSERHTFLATQRDDLLAAKNQLFETMDEMDDEV
                     RTRFKEVFEAIRQEFKIVFPNMFGGGKAELVLTEPTDLLKTGIEIEVQPPGKKLQSLS
                     LLSGGERALTAIALLFSIIRVCPVPFCILDEVEAALDEANVKRFGRYLSDFQDDTQFI
                     VVTHRKGTMVAADVLYGVTMQESGVSKIVSVRMEDINEEAKINETKV"
     gene            45287..46096
                     /locus_tag="IWO89_000597"
     CDS             45287..46096
                     /locus_tag="IWO89_000597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HAQ0455378.1"
                     /translation="MIKLVAIDLDGTLLNEQKLISDENKQALAQAKQQGVKIVLCTGR
                     PLAAMAHYLQELGLVDEGDYSITFNGGLVQKNDTGEIMEKKVMEVSDIQRLYKLAQQL
                     DLPLDVLSDNVVLQLPSAPRKQSLYNVLNKLLQFQPADISDITDEFVLNKAVIAYDQE
                     ELDPKIKEIPAEFHEMYEIIKTRSMLLEFMPKGVTKAYGISLLAKDLGLKQEEIMAIG
                     DEENDLPMIQYAGLGVAMENAVPFVKEAADFVTASNEENGVAKVIQKFVLE"
     gene            46107..47105
                     /gene="ftsY"
                     /locus_tag="IWO89_000598"
     CDS             46107..47105
                     /gene="ftsY"
                     /locus_tag="IWO89_000598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HAQ0455379.1"
                     /translation="MGFFDKIKRAFTGEEKTEEVEVQEKYDKGLEKTRKTFGERMNEL
                     FANFRTVDEDFFEELEETLIGADVGFETAIRITEALRQEVKLRNAKKSAEVQNAIIEK
                     LVDLYEAEGINEVNELNIQEDGLTVILFVGVNGVGKTTSIGKLAHQFKNEGKKVLMAA
                     ADTFRAGAIDQLVVWGERAGVEVVRGNAGGDPAAVVFDALERAKTEQADVLLVDTAGR
                     LQNKVNLMNELEKIKRVIQRELPEAPQETLLVLDATTGQNAMVQAKQFKETTDVTGLV
                     LTKLDGTAKGGIVLAIRNELHLPVKLVGLGEGIDDLEPFDPNDFVVGLFKGLLKEE"
     gene            47292..49559
                     /gene="gshAB"
                     /locus_tag="IWO89_000599"
     CDS             47292..49559
                     /gene="gshAB"
                     /locus_tag="IWO89_000599"
                     /EC_number="6.3.2.2"
                     /EC_number="6.3.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glutamate--cysteine ligase
                     GshA/glutathione synthetase GshB"
                     /protein_id="HAQ0455380.1"
                     /translation="MMNFKQLLLHVNARPFIDQARFGIEREGQRVDLAGNLAKTDHPA
                     IFGDRSYHPYIQTDFSETQTEMITPVTDSIPELFQYLAAVYDVTARSIPKEEMIWPLS
                     MPPALPEKDEEIIIAKLKNFEDVLYRRYLAKEYGKRKQMVSGIHFNFEFGDELLRTLF
                     SHQEEFQDFSEFKTELYLKTARNFMRYRWMITYLFGASPMSEKNYFLDESHPQEPVRS
                     IRNSALGYTNHPNVKVSYASMKQYLADIERMIEEGKLSEEKEFYTPLRFRGGKKVADL
                     ATTGVRYIELRNIDLNPYARLGINPEQVRFLQLFLMYMLWTEEKEDCDQWVAEGTTRN
                     NKVALEQPSDQTEFHQEGREILEGMKQMLVELDWLDSLYLVEEALTQMDHPEQTLAAK
                     LYQEAQLSSQQEVAVALGHQYYKESHERPYQLAGFREMELSTQIFMFDAIQKGVQVKV
                     LDESDQFLRLQFQDHVEYVKNANMTSKDSYIVPLIMENKTVTKKVLKEAGFRVPGGAE
                     FSSMEEAVKAYPRFAEQAFVIKPKSTNYGLGITIFKEGASLEDYQAGLAIAFREDSSV
                     LVEEFMPGTEYRFFVIDGEVQAIMLRVPANVIGDSIRTVKELVEEKNSDPLRGTNHRA
                     PLELIQLGELEQLMLKEQGLTIESVPQANQIVYLRENSNISTGGDSIDMTDEFSEAYK
                     KIAVSAVEALGAKISGIDLIIPDKEIDPTTDKKAYGIIEANFNPAMHMHVYPFAGKGR
                     RLTMNVLKLLYPEVF"
     gene            complement(49648..50100)
                     /locus_tag="IWO89_000600"
     CDS             complement(49648..50100)
                     /locus_tag="IWO89_000600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YueI family protein"
                     /protein_id="HAQ0455381.1"
                     /translation="MAQDDVKSHLDKGLYGTPLVNPEEQHKYLGTFRERCYLSMTVAE
                     MIQPKNKENFLKELKKHPDASILLNGALPLSVQNQYIQLATKNNSRFTVVNDYVTANP
                     EEFGLLLTAKEAVNEPVIDIEKKYPEENSSPAEKEKPKKKKSFWDRLF"
     gene            50250..51200
                     /locus_tag="IWO89_000601"
     CDS             50250..51200
                     /locus_tag="IWO89_000601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron chelate uptake ABC transporter family
                     permease subunit"
                     /protein_id="HAQ0455382.1"
                     /translation="MRYGRLVALLVILSSISLFIGVSNVQLSELLQLDGQSLQILLVT
                     RLPRTICLILVGATSSICGLIMQHLTQNKFVSPTTAGTMDSARLGILVAMIFLPGASL
                     IVRSLTAFCFAFAGTLLFLSLTRLFLQKNQLMLPLIGVMLGNIIGSVATFFAYQFQLV
                     QNMTSWLQGNFATVIRGNYELLYAVVPLLLLVWLFAYRFTIIGLGESFAVNLGIDYQK
                     MQFLGIGLVSLASAVMLIMVGSIPFIGVIIPNLVSRIYGDQVHKTIGVTALAGSLFLV
                     FCDLVARVLIFPYEIPVSVIVGIIGGAIFLYLLIRGRRYA"
     gene            51193..52152
                     /locus_tag="IWO89_000602"
     CDS             51193..52152
                     /locus_tag="IWO89_000602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron chelate uptake ABC transporter family
                     permease subunit"
                     /protein_id="HAQ0455383.1"
                     /translation="MLKKKPSIFIGMLVLVLFVTGMYLTIHTYGNWHFACALRSKKIF
                     AFILVSLAVSSATISFQTITHNQFLTPSVLGLESLYVLVQTVLFFFIGGVTMLQQTGT
                     VFFLLNVGLMVGLSLLLFFFLLKKMRANLFLLLMSGMILGTLFGSVSTFLQVIMDPNE
                     YDLLQGRLFASFGNIQSQYLLPASLIIIGCWYLLFRYRHVLDVFHLGNEQAVNLGIDT
                     DRFEFLILCCVSLLTGTSTALVGPVTFLGFIVANISYRVFSTYRHGQLFIGSFLVAML
                     FLTGGQLAVEQIFGWNTTVSVVVEFVGGIYFVTKLIRERKSIL"
     gene            52149..52904
                     /locus_tag="IWO89_000603"
     CDS             52149..52904
                     /locus_tag="IWO89_000603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAQ0455384.1"
                     /translation="MIDLQKVNKLYHGKSVVKDVQLTIPEKKLTAFIGPNGAGKSTVL
                     SMISRLIPKDTGEIYLDHNEVKAWKSNELAKKLSILRQSNEINVQITVKELVEFGRFP
                     YTKGKLTTKDEEIVQESLEHLGLEEIADQSIDTLSGGQLQRAYIAMVLAQDTDYILLD
                     EPLNNLDMNFAVQIMQILKNLVQEIGKTIVIVLHDINFAASYADEIVAMKDGQLFTQG
                     TTQEMIKKDVLDELYEMDIRICEIEGRRFCLYF"
     gene            52927..53880
                     /locus_tag="IWO89_000604"
     CDS             52927..53880
                     /locus_tag="IWO89_000604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="siderophore ABC transporter substrate-binding
                     protein"
                     /protein_id="HAQ0455385.1"
                     /translation="MKKLVVGILILASFGLAACGNSNNTSQADTKKSSTQTETTLTIT
                     DSNGDQIEVPNNPEKVVVFDNGSLDTMDALGVGDKVVGAATSSLPEYLSSYKKVESAG
                     GIKEPDLEKINQLQPDLIIISGRQRDFQKDLSQIAPTIFLSLDAKNPWESFQQNVTAL
                     GEIFGKQEEAKTQLEELSSAIDQTKKKAEATDKKALVTLVNEGQLSAYGSGSRFGFIH
                     DLFGFEQADDQIEASTHGQSVSYEYVLEKNPDILFVVDRTKAIGGDDSKDDISANELV
                     AQTNAGKNQQIISLEPDVWYLSGGGLESMKLMIEDVNQAFK"
     gene            complement(54403..54699)
                     /locus_tag="IWO89_000605"
     CDS             complement(54403..54699)
                     /locus_tag="IWO89_000605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanyl-tRNA synthetase subunit alpha"
                     /protein_id="HAQ0455386.1"
                     /translation="MKEYIKNIYFIEETQNIEGSYIEVKTLFVNEDKTKALDIYKKLA
                     SKKTNSFGLILSEYKIKAEESYFYQLLKRWSKLPADFYRKMQIINYQPLAETHA"
     gene            55054..55410
                     /locus_tag="IWO89_000606"
     CDS             55054..55410
                     /locus_tag="IWO89_000606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster biosynthesis family protein"
                     /protein_id="HAQ0455387.1"
                     /translation="MKLEVTKEAQEVLKNKLEADDQLLLDIENGDGPFAESQVSCQLD
                     TAFRLIIVKKDTQTDLSLYSVVADTPVGPIKIKDSAILYMDDPSTLALEPTYNSLQLK
                     GPSGLRKGNLQVVRKD"
     gene            55420..56319
                     /locus_tag="IWO89_000607"
     CDS             55420..56319
                     /locus_tag="IWO89_000607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphohydrolase"
                     /protein_id="HAQ0455388.1"
                     /translation="MGKLAIISDLHVDINKFGESELMQLWQVLQKHKITRLHLAGDTA
                     NKVDRCLAVVEFFVNKGLSTTFHFGNHELADVPNEQMMDHFPDPHFLNERYIALNEQT
                     VLLGMNGWYDYQFSELKDYEAIVRMKRLYWYDRFIQRGKTDLEVNAEMLAAAKNLLDQ
                     LEAKKLEVILSTHFVPKRAFIMYQSAPYERWNKLNAFLGSESFGELLDRYSNIKQVVF
                     GHTHRRFEDKSIGHTIYSCRPFGYYYEWQLTRDFVLKEQLLDSYDPMKLRVLLRAHHP
                     AFQAYQTQHLQEEFEQAMTIVPY"
BASE COUNT        18544 a        10222 c        12115 g        15642 t
ORIGIN      
        1 agttggcagg agaaaaccgt ttatacgaaa aaagggactg tgacaaatcg tgtcacagtc
       61 ccttttttat agtgtttttt ataggtagta ttttttagca actgttaagt catcatttaa
      121 ttcataaaca agtggttgac ctgttggaat ttcaagatcc atgatgtctt cgtcagagat
      181 accttcgata tgtttagcta gggcgcgtaa tgagttacca tgtgcagcta ctaaaacagt
      241 tttgttgtct agtaatgctg gagcaatttc atcttgccag aatggcaaag cgcgttctaa
      301 tgtcactttc aagttttctc caccaggaat atcgcgtttg tctaacattg cgtaacgacg
      361 gtcatttgct gcagaacctt cgtcagtttc ttccattaat ggaggaagag tatcgtaaga
      421 acgacgccag atgtgcactt gttcgtcgcc gtatttttca gctgtttctt ttttgttcaa
      481 accttgtaat ttaccatagt gacgttcgtt taagcgccat gatttgattt gtggtaccca
      541 aagttgatct gaatattcta agatcaagtt acatgttttg attgcacgag ttaaaacaga
      601 agtgtaagca acatcaaatt cgattccagc ttctttgatt ttgcgtccgc cttcttttgc
      661 ttcttcgatt ccttctggtg ctagatttac atcagcccaa ccagtaaatt ggttcaatgc
      721 attccattca ctaagaccgt gacgagaaaa aactaatttt ggcatgttta gctctccttt
      781 taagataggt ttatatttct gaaattttca gttctgtttt attgtacaat tttttggaag
      841 agatttccat cgaaaaatta gataagattc tatgagaaaa caagtagaga atataaaaaa
      901 acgaacagtg cccacgcaac tgttcgtttt taggtattag atgcataatt gctcgatttc
      961 tttttcaggt aaattgatat atttataaca tgttccaact gaaacaccgc attttgaaga
     1021 aataaattgg atcgtttctt ttttttcata atagagacgt cggatttttt tgatcgtttt
     1081 tgcatcgatt tttggtcttc cgccgatttt tcctttttta cgagcttcat ctaatccaac
     1141 taatgtacgt tctttgatca atgcgcactc catgtttgct aggttttcca ttaattggaa
     1201 atagattttt cccatttcgc gcgtatcgat tccttcatca agactaatca agtgcagccc
     1261 gcgatctttg aataattgcg tgagttctgt taattgccgc gtagatttgc ctaatcgatg
     1321 aagttggcaa atgactagtg catcgccttg ttgcatgtca gcgatcactg actccaaatg
     1381 aacagcagta gaatcatttt gttccacttc atatgtctca tggaaaattt cttgacaacc
     1441 aaagtcagat aattgatcaa cctgagttgt taaatcaacg tcatttatgg ttgtgcgtgc
     1501 ataaccaata attttcatgg tgattccttt ctagcagtga ttttctaact ggatataaca
     1561 gtaaaaatgt agcataaagt acgtcaaaaa gatagttttt tcgcaaattt tccgctgtct
     1621 ttttttattg gatgatcaag cagtttttct ttatttacga cggattgcgt caagaatctc
     1681 aacgcctttt tctgagccga caactacttt agaaaccatg tttaggaaaa gcccatgttc
     1741 tactacccca acaagttcat ctaaataggt agctaagctt tctggctgtt cgatgacttc
     1801 tagatgcaga tcaatgatgt aatgaccacc atctgtggtt aagacttctc cgttttcgtc
     1861 tgtacgcaag acgggttgga atcttttttc ttcaaaaata tgaagtagct gttgggagcc
     1921 ataagggatc acttcaactg ggagtgggaa cttcccaagt ttatcgacta atttagaaga
     1981 atcaacgatc caaatcgttt cttttgagta cgtagcgact attttctcga aaagcaaagc
     2041 agcgccgccg cctttgatcc cttgaaaatc agcactgatt tcatcagcac cgtctattgt
     2101 caaatcgact acggggactt catcaatact ctttaacggt attcctaatt tttccgcttg
     2161 ttcttttgtc gcatttgaag tagtaactcc tgtgatacgt aaaccttctt cacgcatacg
     2221 gcgaccaatc tcttctacca tataataggc ggttgaaccg gtcccgagac ctacgatcat
     2281 cccgtccttt acgaatttcg cggcttcaat gccagctaat tgttttaaat tcatctttgc
     2341 gtccaccttt ttttagtttg agcatcgtgc tttcctcact attttagcgt gatttacggt
     2401 aaaaagatac cattcttttt tattccaata aagctttttc aaaaaaaatt gcatctcttg
     2461 ttgacatgtt ttttcattcg tgatattcta acaaaggtgc tttatctaca ggtttctgtg
     2521 aagaaacgtg ctgactgttt atcaaagcat atttgataag gatgcagaca ttgcatttat
     2581 tttttatcac aaaaaaagaa ccacctggat gtgtggaact agacgcaaat cgtgggaagg
     2641 aggaaacaag gaatgcctac aattaaccaa ttagtacgta aacctcgtaa atcaaaggtg
     2701 gaaaaatcta attcacctgc tttgaacaaa ggatataaca gctttaagaa aactcaaact
     2761 aacgtaaact cacctcaaaa acgtggggta gcaactcgtg tgggtacaat gacacctaaa
     2821 aaaccgaact cagctttacg taaatatgca cgtgttcgtt tgtcaaactt aattgaagtt
     2881 actgcttata tcccagggat tggtcataac ttacaagaac acagtgttgt attattacgc
     2941 ggtggtcgtg taaaagactt accaggggta cgttaccata tcgttcgcgg tgctttagat
     3001 actgctggtg ttaacgaccg taaacaaagc cgttctaaat acggaactaa aaaacctaaa
     3061 gcttaatttt aattattaaa caaatgaaac ttgcttctac tatatagaag taattactat
     3121 gaacaatatt tcggaaggag gagttacgga tgccacgtaa aggtcctgtt gcaaaacgtg
     3181 atgttttacc agatcctatt tataactcaa aattagtaac tcgcttgatc aaccgtgtaa
     3241 tggttgacgg aaaacgcggg attgcagcta acattatcta taatgccttt gacttgatca
     3301 aagaatctac aggtaacgat ccattagaag tgtttgaaca agcaatgaaa aacgttcaac
     3361 ctgttcttga agttaaagca cgtcgtgttg ggggttcaaa ctaccaagta ccagttgaag
     3421 ttcgccctga acgccgcgtt actctaggtc ttcgttgggt agtaaactac gctcgcttgc
     3481 gcggtgaaca cacaatggaa gaacgcttag caaaagaaat catggatgct gcaaacaaca
     3541 caggtgcttc tgttaaaaaa cgtgaagaca ctcataaaat ggctgatgcc aaccgtgcgt
     3601 ttgctcacta tcgctggtaa gattctctct gtttggtcgg cttttataag tcgacctcag
     3661 atagtgtcct tatttttagc aactagatag taaagagagg agaaacaagg agaatgacca
     3721 gagaattttc tttacaaaac actcgtaata tcggtatcat ggcccacatc gatgctggta
     3781 aaacgactgc tactgaacgt atcttgtact atactggtaa aatccataaa attggtgaaa
     3841 ctcatgaagg agcttcacaa atggactgga tggaacaaga acaagaacgt ggtattacga
     3901 tcacttctgc tgctactaca gcacaatgga agggtcaccg aatcaacatc atcgacacac
     3961 caggacacgt agacttcaca gttgaagttg aacgttcatt gcgtgtactt gatggtgctg
     4021 taactgttct tgatgcacaa tcaggtgttg aacctcaaac tgaaacagtt tggcgtcaag
     4081 caacaactta tggtgtacct cgtatcgtat ttgcaaacaa aatggacaag atcggtgctg
     4141 acttcttata ttcagtatca actttacatg accgtttgca agcaaacgct cacccaatcc
     4201 aattaccaat tggtgcggaa gacgacttca caggaattat cgaccttgta acaatgaagg
     4261 ctgaaatgta tacaaacgat cttggaacag agatcgaaga aacagaaatt cctgaagaat
     4321 accgcgaatt agctgaagaa tggcgcgaaa aattggttga agctgtagct gaaactgatg
     4381 aagaacttac tttgaaatac ttagaaggtg aagaaatcac tgaagctgag ttaaaagaag
     4441 gtatccgccg cgcaacagtt aacgtagaat tctacccagt actatgtggt tcagcattta
     4501 agaacaaagg ggttcaatta ctattggacg ctgttcttga ttacttgcca tcaccgttag
     4561 atatcccagc tatcaaaggg attgacccta aaactgatga agaagtagaa cgtcctgcag
     4621 acgacagcgc tcctttctca gctttagcat ttaaagttat gactgaccca ttcgtaggtc
     4681 gtttaacttt cttccgtgtg tactctggtg ttttacaatc aggttcatac gtacaaaacg
     4741 caacaaaagg caaacgtgaa cgtgttggtc gtatcctaca gatgcatgcc aactctcgtt
     4801 cagaaatctc tgaagtttac gctggagata tcgctgctgc tgttggtttg aaagacacaa
     4861 ctactgggga cacactatgt gatgaaaaga gtctagttat tcttgaatcc atggaattcc
     4921 cagaaccagt tatccaagtc gctattgaac ctaaatcaaa agctgaccaa gataaaatgg
     4981 gtgtggcttt acaaaaacta tctgaagaag atcctacttt ccgtgctgaa acaaacgtgg
     5041 aaacaggcga aacaatcatc gctggtatgg gtgaacttca cttagatatc atcgttgacc
     5101 gtatgcgtcg tgaattcaaa gttgaagcta acgtaggtgc gcctcaagta tcttaccgtg
     5161 aaactttccg tgctggcact caagccgaag gtaagtttgt acgtcagtct ggtggtaaag
     5221 gtcaatacgg tcacgtatgg attgaattta caccaaatga agaaggtgct ggtttcgaat
     5281 ttgaaaatgc gatcgtcggt ggggttgttc ctcgtgaata cattccagct gttgaaactg
     5341 gtttgaaaga tgctatggaa aatggtgtct tagctggcta tccattagta gatatcaaag
     5401 ctaaacttta cgatggttca taccatgatg tcgattcaaa tgaaactgcc ttccgtgttg
     5461 ctgcttctat ggcattacgt gcagcagcta agaaagctaa tccagtaatc cttgaaccaa
     5521 tcatggctgt agaagttgtt atccctgaag attatttagg tgatgttatg ggtcacgtaa
     5581 ctgctcgtcg tggacgtgtt gaaggtatgg aagctcgcgc tggcggccaa caagttgttc
     5641 gtgcgatggt tccacttgct gaaatgtttg gttatgctac aactcttcgt tctgctactc
     5701 aaggtcgtgg tacatttaca atgacatttg accactacga agatgtacca aaatcagtac
     5761 aagaagaaat cattaagaaa aacggcggaa aagctgaata atttcttaaa accggatagt
     5821 cctgcaaaga gattatccaa agagactata tttttaatat agtttcgtgt aaaataatta
     5881 tcgatgatat gggcatgtgg tgtaccttgt gtacatcata tacctatcag acaaataaaa
     5941 aatatattgt atttttagga ggacatttta aaatggcaaa agaaaaattt gaccgttcta
     6001 aaccacacgt taacattggt actatcggac acgttgacca tggtaaaact acattaacag
     6061 ctgcaatcac aactgtacta tctaagaaaa acggcggcca agctatggct tacgatcaaa
     6121 tcgatggtgc tccagaagaa cgcgaacgcg gaatcacaat ctcaacagct cacgttgagt
     6181 atgaaacaga tacacgccac tacgctcacg ttgactgccc aggacacgcg gattacgtta
     6241 aaaacatgat cactggtgct gctcaaatgg acggagctat cttggtagtt tctgctgctg
     6301 acggcccaat gcctcaaact cgtgaacaca tcctattgtc tcgtcaagtt ggtgttcctt
     6361 acatcgttgt attcttgaac aaagtagaca tggttgatga cgaagaatta ctagaattag
     6421 ttgaaatgga agttcgtgac ctattaacag aatacgaatt ccctggtgac gatgttcctg
     6481 tagttgctgg atcagctttg aaagctctag aaggcgacgc ttcatacgaa gaaaaaattc
     6541 ttgaattaat ggctgcagtt gacgaataca tcccaactcc agaacgtgac aacgacaaac
     6601 cattcatgat gccagttgaa gacgtgttct caattactgg acgtggtact gttgctacag
     6661 gtcgtgttga acgtggacaa gttcgcgttg gtgacgaagt tgaagttgtt ggtattgctg
     6721 aagaaacttc aaaaacaaca gttactggtg ttgaaatgtt ccgtaaattg ttagactacg
     6781 ctgaagctgg agacaacatt ggtgctttac tacgtggtgt tgcacgtgaa gacatccaac
     6841 gtggacaagt tttagctaaa ccaggtacaa tcacacctca tacaaaattc tctgcagaag
     6901 tatacgtgtt gacaaaagaa gaaggtggac gtcatactcc attcttcact aactaccgtc
     6961 cacaattcta cttccgtaca actgacgtaa caggtgttgt tgaattacca gaaggaactg
     7021 aaatggttat gcctggcgac aacgtaacaa tggaagttga attaatccac ccaatcgcta
     7081 tcgaaaacgg tactaaattc tcaatccgtg aaggcggacg tacagttggt gccggtgtcg
     7141 ttacagaaat caaagcttaa ttcacttaag ttttaaagta aagcaacatt tattcggacg
     7201 taatcgctcg gaacgaaggt cttaggatct ttgttccgag cttttttatt tattgcagga
     7261 tcaggaaaaa agacaggagt attataaata gaagaaacac gaaataaaag agtcagtatt
     7321 ttctaaatga aactttatga aaacaaaatg ttttttacaa gaaaaagcct tgctttgtga
     7381 gtttatatat attataatga caaaggtgcg tttactttag tgaattgcac agacgtaatc
     7441 agaatgattt cgtcggctaa gaaacgagag gttgcgacac gcccggacgc tttgccacga
     7501 acgagcgtga cggaaatttt cgtggagcta tgtctacttt tcaaaatagg cgaaggaggg
     7561 aacaagatgg caaaacaaaa aattcgtatc cgtttaaaag cgtatgaaca ccgtatttta
     7621 gatcaatcag cggataaaat cgtggaaact gcaaaaagaa ctggagctag cgtgtctggt
     7681 ccaattccat taccaactga acgcagtctt tacacaatca tccgtgcgac tcataaatac
     7741 aaagattcac gcgaacaatt cgaaatgcgt acacacaaac gtcttatcga cattgtgaac
     7801 ccaacaccaa aaactgttga tgctttgatg aagctagact taccatctgg tgtaaacatc
     7861 gaaatcaaat tataaaacta aaacaaaaat aatggaggtg tactcatgac caaaggaatc
     7921 ttagggaaaa aagtgggaat gacacaaatc ttcactgaat ctggtgaatt aattccagta
     7981 actgttgtag aagctactcc aaacgtagta ctacaagtga aaacaatgga aacggatggc
     8041 tacgaagcta ttcaagttgg ttaccaagat atgcgtgaag ttttatcaaa caaacctgcg
     8101 aaaggtcatg ttgcaaaagc aaacacggct cctaagcgct tcattcgtga attcaaaaat
     8161 gttgagctag gagaatatga agtaggaaaa gaaattacag tagatgtttt ccaagcagga
     8221 gacattattg atgtaacagg tactacgaaa ggtaaaggat tccaaggggt tatcaaacgc
     8281 cacggacaaa gccgcggacc aatggctcac ggttctcgtt accatcgtcg tcctgggtca
     8341 atgggtccag ttgcacctaa ccgtgtattt aaaaacaaac gtcttgctgg ccgtatgggt
     8401 ggcaaccgcg taactatcca aaacttggaa gttgtacgtg tcgatgcaga aagaaatgtt
     8461 atcttgatca aaggtaatgt tcctggagct aaaaaatctt taattactat caaatcagct
     8521 gtgaaagcta aataattgta ggaagaaagg aggaactaag gaatgccgaa tgtagcatta
     8581 ttcaaacaag atggaagcca aaacggtgaa atcacactta acgaagaaat cttcggaatt
     8641 gaacctaatg aaagtgttgt ttacgatgca atcgtaatgc aacgtgcgtc attaagacaa
     8701 ggaacacacg cggttaaaaa ccgtagcgct gttcgcggcg gcggacgtaa accatggcgt
     8761 caaaaaggaa caggtcgtgc acgtcaagga tctatccgct caccacaatg gcgtggaggt
     8821 ggcgtagtct ttggaccaac accacgttct tacagctaca aacttcctaa aaaagttcgt
     8881 cgtttagcaa tgaaatctgt attgtcagaa aaagttgctg aaaacaactt ggtagcaatc
     8941 gaaggcttga acttcgacgc accaaaaaca aaagaattca aacaagttct tgctaactta
     9001 tctatcgact ctaaagtatt ggttgttcta gaaaaaggta acgactttgc tgctttatca
     9061 gcacgtaact taccaaacgt ttctgtagta acttctgata atgtgagtgt gctagacgtt
     9121 gtatcaaata caaaagtgtt agcaacacaa actgctctta ctcaaattga ggaggtgctt
     9181 gcataatgaa cttactagac gtaatcaaac gcccagtgat cactgaaaaa tcaatgcttg
     9241 ccatggatga caagaaatac acttttgaag tggacactcg cgcaaacaaa actttagtaa
     9301 aacaagcggt agaagctgct tttgacgtta aagtcaaaaa cgtgaacatc gtgaacgtac
     9361 gtgcgaaatt caaacgtatg ggcaaatatg caggatatac aaaaaaacgt cgcaaagcga
     9421 tcgttacatt gactgaagat tcaaaagaaa tccaattatt cgaagctgct gaataagcac
     9481 taatagataa ctcattaaat taggagggaa aaacgtggcg attaaaaagt acaaacctac
     9541 ctcaaatggt cgtcgtaaca tgacaggttc tgattttgct gaaatcacaa catcaacacc
     9601 tgaaaaatca ttgttacaac cattgaaaaa ccatgctgga cgtaacaaca acggccgcat
     9661 cactgtacgt catcaaggtg gcggacacaa acgccaatat cgcgtgatcg acttcaaacg
     9721 taacaaagat aacgttgttg caactgttca aacgatcgaa tatgatccaa accgttcagc
     9781 taacatcgcg ttagtacact atgaagatgg agtgaaagct tacatcttag cacctaaagg
     9841 actagaagtg ggtatgaaac ttgtttcagg tccagaagca gatatcaaag tcggaaatgc
     9901 tcttccatta gaaaacattc cagttggtac tgttatccac aacattgaaa tgaaacctgg
     9961 taaaggtgga caattgatcc gttctgctgg tacaagtgct caagtacttg gtaaagaagg
    10021 caaatacgta ttgatccgct tgaactctgg tgaagttcgt atgatcttag ctgcatgccg
    10081 tgctacaatc ggttctgtag gtaacgaaca acacgaatta atcaacatcg gtaaagctgg
    10141 acgctctcgt tggatgcgta aacgcccaac tgtacgtggt agcgtaatga accctaatga
    10201 tcacccacac ggtggtggtg aaggtaaagc tcctatcgga cgtaaagcac cagtttctcc
    10261 ttggggtcaa ccagctcttg gttacaaaac tcgtaacaaa aaagctaaat cagacaaact
    10321 tattgttcgt cgtcgcaaaa ataaataatt actttgacca tgtgtcgcac attttgagag
    10381 gaggttcacc atgggtcgta gtttaaagaa aggacctttt gtcgatgagc atttaatgaa
    10441 aaaagttgaa gctcaagcag gggttgaaaa gaaaaaagtt atcaaaactt ggtcacgccg
    10501 ttcaacagtt ttcccaagtt ttgttggata taccatcgca gtttatgatg gacgtaaaca
    10561 tgtaccagtt tacatccaag aagacatggt aggacacaaa ttaggtgaat ttgcacctac
    10621 aagaacttat cgtggacatg ctgctgacga taagaaaacg aaacgctaat ttgaggggag
    10681 gataaatcca aatggcagaa caaatcacat cagctaaagc aactgcaaaa acagttcgca
    10741 cttcacctcg aaaagcccgt ttagtaattg accttatcag aggtaaaagt gtcgcagatg
    10801 caatttcaat cttgaaattc acaccaaaca aatctgctgg aatcattgaa aaagtattga
    10861 tgtcagcagt tgcgaatgca gaaaataact ttgacttaga tgttgaaaac ttggtagtaa
    10921 gcgaagcttt tgttaacgaa ggacctacaa tgaaacgttt ccgtcctcgt gcaaaaggat
    10981 cagcttctcc aatcaacaaa cgtacaagtc atatcacggt agtcgtatca gaaaaataag
    11041 gagggataat cagtgggtca aaaagtacat ccaattggaa tgcgtgtagg catcatccgc
    11101 gactgggatg ctaaatggta tgctgaaaaa gagtatgctg agttcttaca cgaagattta
    11161 agaatccgta aatttatttc aactaaactt gctgatgctg ctgtatcaac agttgaaatc
    11221 gaacgcgcaa caggtcgcgt aaacatttca attcacacag ctaaaccagg tatggttatt
    11281 ggtaaaggcg gttctgaagt cgaaagccta agaaaagaat taaacaaatt aactggtaaa
    11341 agaatccata tcaatatcgt ggaaatcaaa aaaccagatc tagatgcaaa attagtaggt
    11401 gaaggaatcg cacgtcaatt agaaaaccgt gttgctttcc gtcgcgctca aaaacaagcc
    11461 atccaacgct caatgcgtgc tggcgctcaa gggatcaaaa ctcaagtatc tggtcgtcta
    11521 aacggagcag atatcgctcg ttcagaagga tactcagaag gaactgttcc tcttcatact
    11581 ttacgtgcgg acattgatta cgcatgggaa gaagcagata caacatacgg aaaactagga
    11641 gttaaagtgt ggatctatcg tggagaaatt cttccaacta aaaaaaacac tgagaaagga
    11701 gggaaataat catgttagta cctaaacgtg taaaacaccg tcgtgaattc cgcggaaaaa
    11761 tgcgcgggga agctaaaggc ggaaaagaag tagcattcgg tgaatacggt ttgcaagctg
    11821 ttgattcaca ttggatcaca aaccgccaaa tcgaagctgc tcgtatcgca atgactcgtt
    11881 acatgaaacg tggtgggaaa gtatggatta aaattttccc tcacaaatct tatactgcca
    11941 aagcaattgg ggtacgtatg ggttctggta aaggggcacc tgaaggatgg gttgcaccag
    12001 taaaacgtgg taaaatcatg tttgaaatcg caggcgttcc tgaagaagta gctcgtgaag
    12061 cgttacgtct agcttctcac aaattaccaa tgaaaactaa gatcgtaaaa cgtgaggaaa
    12121 tgggtggtga atcgaatgaa ggttaaagaa atcagagaat taaccactgc cgaaatgctt
    12181 gatcaagaaa aacaattaaa agaagaattg tttaatctta gattccaatt agcaacaggt
    12241 caattagaaa acactgcacg tattaaagaa gtacgtaaat cgattgcacg catcaaaact
    12301 gtgttgcgtg aacaagctaa gtaattatgg aaggaggcca ttaagcgtat gactgaagga
    12361 agaaatcaac gcaaagttta tcaaggtcgc gtggtttcag acaaaatgga taaaacaatt
    12421 accgttgctg tcgaaacaaa gaaaaaccac cctatctatg gcaaacgcat gaactattct
    12481 aagaaataca aagtgcatga tgaaaacaac acagcaaaag ttggcgacat cgtgagaatt
    12541 atggaaactc gtccattatc agctacaaaa cgtttccgtt tgttggaagt agtcgaagaa
    12601 gcagttatta tctaataaca cgagattttc ctatatagat tgaatgcaaa atctgaaagg
    12661 aggatacaca acgtgatcca agcagaaagt cgtttaaaag tcgctgataa ctcaggcgca
    12721 cgtgaaattt tgacgatcaa agtccttggt ggatctggtc gtaaaactgc taacatcggt
    12781 gatgtgatcg ttgctacggt taaacaagca acgccaggtg gggttgtcaa aaaaggtgac
    12841 gtcgttaaag ccgttatcgt tcgaacaaaa tcaggagctc gtcgtacaga cggttcatat
    12901 attaaatttg atgaaaatgc tgcagtgatt attcgtgatg ataaaagccc tcgtggaaca
    12961 cgtatctttg ggccagttgc acgtgaacta cgtgaaaaca acttcatgaa gatcgtttca
    13021 ctagcaccag aagttttata atcttgagac acttatcaaa ggaggtgcga aacagttatg
    13081 tttgttaaaa aaggcgataa agttaaagtt atcactggaa aagacaaaaa caaagaaggc
    13141 gttgtactag cagcgttccc gaaaaaagat aaagttattg tcgaaggtgt aaatgtcgtt
    13201 aaaaaacacc aaaaaccttc tcaagctgct cctcaaggtg gaattgttga gatggaagcg
    13261 ccaattcatg tttctaacgt gatggtggtt gactctactg gtgtagctgg ccgagttggc
    13321 tacaaagaag tagacggcaa aaaagtccgt gtttctaaaa aaaccggtga agttttagat
    13381 aaataaaagt ttaaggaagg agggtcttac tgaatgaacc gcctaaaaga aaaatatctt
    13441 aaagaagtaa ctccatcatt gatggaaaaa tttaactata gctctgttat gcaaacacct
    13501 aaagttgaaa aaatcgtcat caacatgggt gttggtgatg cggtatcaaa cgctaaaaac
    13561 ttagataaag cagttgaaga attaactttg atcactggtc aaaaaccaat gattacaaaa
    13621 gctaaaaaat caatcgctgg attccgttta cgtgaaggaa tgccaattgg tgcaaaagtt
    13681 acattgcgcg gagaaagaat gtacgaattc ttagacaaat tggtttctgt ttcactacca
    13741 cgtgtacgtg acttccatgg tgtaagtaaa aaagcctttg acggtcgtgg aaactatact
    13801 ttaggtatta aagaacaatt gatcttccca gaagtagatt atgatttagt agataaagta
    13861 cgtggtatgg acatcgttat tgtaacaaca gcaaactctg atgaagaatc tcgtgagttg
    13921 ttgacacaat taggcatgcc attccaaaaa taattaaagg aggcgaacta cgtggctaaa
    13981 aaatcaatga ttgctaaaaa caaacgtcct gcaaaatttt caacacaagc ttatactcgt
    14041 tgcgaacgtt gcggacgtcc acattcagtt tatcgtaagt tccatctttg ccgtatttgc
    14101 ttccgcgaac ttgcctataa aggtcaaatt cccggcgtga agaaagctag ctggtaaaaa
    14161 gtaaattcgc ataagaagga ggtaaaagat caatggtcat gacagatcca attgcagatt
    14221 ttctaactcg tatccgtaac gcaaacatgg ttaaacatga gtctttagaa gtacctgcat
    14281 caaaaattaa acgtgacatc gctgaaatct tgaaacgtga aggtttcgtt cgtgatgttg
    14341 aatacatcga agatgacaaa caaggcgtga tccgtgtttt cctaaaattt ggtaaaaacg
    14401 gcgaacgtgt tatcacaaac ttaaaacgta tctctaaacc aggtttgcgt gcttatgtca
    14461 aagctgacga agtaccaaaa gtattgaacg gtttaggtat cgcaatcatt tcgacttctg
    14521 aaggtgttgt cactgataaa gaagctcgtg aaaaaaatat cggcggcgaa gttatcgctt
    14581 acgtatggta atcaagtaga atctacaagg aggtgtcttt aagtgagccg tatcggaaat
    14641 aaagtcgttg ttatccctga aggagtaaca gttactcaag acggaaacaa cgttacagtt
    14701 aaaggaccaa aaggtgaatt gactcgtact ttttcagcag atatcaaaat gaatatcgaa
    14761 ggaaacgaag taactttcac tcgtccaaac gacagtaaag aaatgaaaac aattcatgga
    14821 acaactcgtg cgaacttcaa caacatggtt gtaggtgtca gcgaaggttt ccaaaaagcg
    14881 ttagaactaa tcggggttgg gtaccgtgca caattacaag gatcaaaact tgtattgaac
    14941 gttggttact cacatccagt agaaatcaca ccaccagctg gagtaactgt tgaagtacca
    15001 gctaacacac aagtaattgt taaaggtgcg aacaaagaag aagttggcga attagctgca
    15061 aacattcgtg gaactcgtcc tccagaacct tataaaggta aaggtattcg ttatgttggc
    15121 gaatatgtac gccgtaaaga aggtaaaact ggtaaataat cgtttagaaa aattgcatag
    15181 aagccagcag caggaaaaag gcatttggct gaagtttacc ttcaaaaaaa tacattttta
    15241 cttgtgccgc agcttctatt tttcgaaacg tatttactgc ttgaaatagt agtacaataa
    15301 aaataaagag gtgacaattg tgattacaaa accagataag aacaaaacac gtcaaaagag
    15361 acatcgtcgt gtacgtaaca atatctctgg tactgctgag cgcccacgct tgaacgtttt
    15421 tcgttctaac aaaaacatct acgctcaaat tattgatgac gtagcgggtg taacgctagc
    15481 aagtgcctct accttggata aagaaatttc aggtggaaca aaaacagaag cagcacaagc
    15541 tgtcggtaaa ctagttgctg aacgtgcagc tgaaaaaggc attaaagtag tagtctttga
    15601 ccgtggtgga tacctttacc atggccgtgt gcaagcttta gctgaagctg ctcgcgaaaa
    15661 tggactagaa ttttagaaga aggaggaata ccattcatgg tttatatcga tccaaaacat
    15721 ttggaattag aagaccgcgt tgttgcaatt aaccgtgtaa caaaagttgt taaaggtgga
    15781 cgtcgtctac gttttgctgc tttagttgtt gtcggtgaca aaaacggaca cgtagggttt
    15841 ggtacaggta aagctcaaga agttcctgaa gcaatccgta aagcaattga agatgcgaag
    15901 aaaaacttag ttgaagtacc tatggttggt tcaacaatcc cacacgaagt aatcggtgct
    15961 tttagtggtg gtagaatcct aatgaaacct gctgtagaag gttctggggt tgctgctggt
    16021 ggaccagttc gtgccgtatt agaattagct ggggtagcag atatcacttc taaatcttta
    16081 ggctcaaata caccaatcaa cgttgttcgc gcaacagttg aaggtttgaa acaattgaaa
    16141 cgtgcagaag aagttgcaga acttcgcggc aaatcagttg aagaattaat cggataagga
    16201 ggacaacaat aatggctgaa ttaaaagtta ctttaaaacg cagtattatc ggacgtcctc
    16261 aaaaccaacg cgatacagtt aaagcgttag gtcttacaaa gattaacagt tcagtggtta
    16321 agccttctaa tgaagcaatt aaaggcatga tcaatactgt ttctcaccta gtggacgttg
    16381 aagaaattta atttagaaaa atcataacta gaacattaag gaggtgccaa acctatgaaa
    16441 cttcatgaat taaaacctgc tgaaggttca cgccaagtac gtaaccgtgt tggtcgtggt
    16501 acttcatctg gtaacggaaa aactgccgga cgcggacaaa aaggacaaaa agctcgttca
    16561 ggcggcggtg tgcgtttagg atttgaaggg ggacaaacac cattgttccg tcgtttacca
    16621 aaacgaggat tcacaaacgt gaaccgtaaa gactatgcag tcattaatct tgatgtattg
    16681 aaccgctttg aagacggagc tgaagtaact cctgttgctt tagttgaagc tggaatcgtg
    16741 aaaaacgaaa aagctggaat caaagtatta gctaatgggg aactaaacaa gaaattaact
    16801 gtgaaagcag caaaattctc taaagcagct caagaagcaa ttgaagcggc tggtggatca
    16861 atcgaggtga tctaatgttt agactattaa aagatgcttt taaagtaaaa gacattagat
    16921 cgaaaatcct atttactgta tttgtgttgt ttgtatttcg cctaggtgca catattactg
    16981 taccaggcgt aaatgctgct tcattgcaaa acctttcttc tttgccattt ttcaacatgt
    17041 tgaacttggt aagtggtagt gcgatgcaaa acttttccat tttctcaatg ggggtttcac
    17101 catacattac tgcttctatc gtcattcagc ttcttcaaat ggacatcgtg cctcgatttg
    17161 ttgaatggtc gaaacaaggg gaagttggtc gtaaaaaact gaatcaagcg actcgatatc
    17221 tgacattagt attggcattt atccaatctg tcggtatcac tgctggattc aatcaatacg
    17281 ctcaattagg tttcgtgaat aatcctaacg tagcaactta cgtaacgatc ggtttgattc
    17341 taacagccgg tacgatgttt gttacttggc taggagaaca gatcacagaa aaaggtatcg
    17401 gaaatggcgt atcaatgatc atttttgcag gtatcatttc tagattgcct gaaagtatca
    17461 gagaaattta tgaagattac tttataaata ttgatccttc agacatatgg aaatctgcta
    17521 tttttgtggc cattttacta gtagcggtct tgttgatcgt gacattcgta acatttttcc
    17581 aacaagctga acgcaagatc ccgatccaat atacgaaacg cgtagctggc gcgcctacaa
    17641 gcagctatct gccgttgaaa gtaaatgcag ctggagttat tccagttatc tttgctagtt
    17701 cgtttattgc tacgccaaat gctgttttac aagcattgag cggtagattc ggttctgaaa
    17761 actggtacaa agtgatgatg gagatcttta actataatac agcacctggt gctacgattt
    17821 atacgattct gatcgttgcc ttcactttct tctacgcctt tgttcaagta aaccctgaga
    17881 aattagcgga aaacttgcaa aagcaaggaa gttatatacc aagcgtacgc cctggtaagg
    17941 ggactgaaga gtatgtctca agattactga tgcgtttaag tacagttggt gcagtcttcc
    18001 ttggtttagt ggcattgctt ccaatcattg ctcaaatggt ttgggatctt ccacaatcaa
    18061 tcggattagg cggtacaagc ttactgatcg ttatcggtgt agctttagaa actgcgaaac
    18121 agttagaagg cttaatgctg aaacgtaaat atgttggatt cattgagtaa gaaagcgtgt
    18181 tgaggtttcc cctcaacaca cgttctcaca catttattag gagggaaaac aatgaacctc
    18241 attttaatgg ggctgccagg cgcaggtaaa ggaacacaag ctgaaaaaat cattgatgct
    18301 tatggtattc cgcacatctc aacaggagat atgttccgcg cagcgatgca aaacgaaaca
    18361 gctcttggcc tggaagcaaa gtcttatatg gataaaggtg cattagttcc tgatgaagta
    18421 acaaatggaa tcgtgaaaga gcgactagct gaaccagata cagagaaagg cttcttatta
    18481 gatggctttc cacgtacgct agatcaagca aaagctctgg acgcaatgct aaaagatttg
    18541 aataaaaaaa ttgatgctgt catcgatatc catgttggtg aagaaatctt ggtcgaacgt
    18601 ctagccggcc gatttatctg cagcaattgt ggagcaactt accataaaat tttcaaccct
    18661 acaaaagttg aagacacatg tgatcgttgc ggtgggcatg aattctatca aagagaagat
    18721 gataaacctg agacggttaa aaatcgtctg gctatcaaca tcaaaaatag tgaaccaatc
    18781 ttggcttatt ataaagaaca aggtttgcta aacactattg atggtgctcg tgaaatcgat
    18841 gctgtatttg cagatgtaca aaaaatcatt gaaaaatagc acgattgccc gtagccgact
    18901 tgcattatgc agttactctt gtcaagttca ataaattatg atagaatatg tcagtccgac
    18961 ttctagagaa tcctctagat gatccatttt gagaattttg aggtgggaac tgcagtttcc
    19021 gccattttat cgtgtgaaaa tgcgaaacaa gtacaaggag gtactgtgcg tggcaaaaga
    19081 agatatgatt gaagtcgaag gtacagtcgt cgaaactttg ccgaatgcaa tgtttaaagt
    19141 tgaactagaa aacggacacc aagttcttgc tactgtttca ggtaaaattc gtatgcatta
    19201 catccgtatc ttacctggtg acaaagtaac ggttgaattg tctccatacg atcttaaccg
    19261 tggccgtatt acttatcgct ttaaataatt gtactccgta aaactacagg aggtattatc
    19321 atgaaagtaa gaccatcagt aaaaccaatg tgcgaacatt gtaaagtaat tcgtcgtaaa
    19381 ggacgcgtta tggtgatttg cccagcaaat ccaaaacata aacaacgtca aggataattg
    19441 gaggtgctat aaatatggct cgtattgcag gagtagatat tcctcgtgat aaacgtgtag
    19501 tagtttcact tacttatatc tatggtattg gtaacactac tgctaaaaag atcttagctg
    19561 atgctggtgt atctgaagat gttcgcgttc gtgaattaac gaatgaacaa acagatgcta
    19621 tccgtgcaga agtagataaa ttaaaagttg aaggggatct tcgtcgtgaa gtgaacttga
    19681 acatcaaacg tttgatggaa atcggttctt accgtggaat ccgtcatcgc cgtggattgc
    19741 ctgttcgtgg acaaaacacg aaaaacaatg cacgtactcg taaaggccca gctcgcgcta
    19801 tcgctggcaa gaaaaaataa ttcttaagtg aaggaggtta aaacttcatg gcagcaaaaa
    19861 aagtgaatcg taaacgccgt gtgaagaaga atatagaagc aggtattgca catatccatt
    19921 ctacattcaa taacactatc atcatgatca ctgatactca cggaaatgca ttagcgtggt
    19981 catcagctgg ttcattaggt ttcaaaggaa gcaaaaaatc aactcctttt gccgctcaaa
    20041 tggcagcaga aactgctaca aaagcagcaa tggaacacgg attaaagact gttgaagtaa
    20101 cagttaaagg acctggttct ggacgtgaag cagcaattcg ttcattacaa gctacaggtt
    20161 tagaagtgac tgcaattcgt gacgtgactc cagttcctca taatggtgcc cgccctccaa
    20221 aacgccgtcg tgtttaatga gtgccgctca ttttgagttt tatcagtaac gtcattgaac
    20281 aagacacttt tcgttttgaa aggggtaaag ataagaatga ttgagttcga aaaaccaaga
    20341 atcgcaaaaa ttgatgaaga aaaagattat ggcaagttca tcgttgaacc tcttgaaaga
    20401 gggtatggga ctactttagg gaattcccta cgtcgtattt tattatcttc tttgccaggt
    20461 gctgccatca ctaatattca aatcgatggt gtactgcatg aattctctac catcaaaggt
    20521 gttagagaag acgtcactca aatcatcttg aacattaaag gcttagcgct gaaaatgtat
    20581 ggccaagaag aaaaaaccct tgaaatcgat attaccggac ctgctacagt aactgctggc
    20641 gatattatcg ttgatagtga tgtagaaatt cttaacaaag atatgtacat ctgtagtgtc
    20701 tctgaaggag ctacgttcca tgctcgctta acagtgaaac ctggacgtgg ttatgttcaa
    20761 gcagatgaaa ataaaaaaga agatatgcca attggcgttc ttccagtcga ttcaatctac
    20821 acacctgttc gtcgtgtgaa ctatcaagtt gagaacacac gtgttggaca ccgtgaagat
    20881 tttgataaat tgacgatgga gatttggaca gatggttcaa ttgaaccgtt ggaagcaatg
    20941 agtttgtctg cgaaaatcat gactgaacat ttggatattt ttgtgaactt aactgatgaa
    21001 gcaaaaaatg ctgaaatcat ggtcgaaaaa gaagaaacac aaaaagaaaa aatgttggaa
    21061 atgacgatcg aagagttaga cttgtctgtt cgctcttaca attgtttgaa acgtgcagga
    21121 atcaatactg tacaagaact tacaaataaa tctgagcctg aaatgatcaa agtccgtaac
    21181 ttaggacgta aatcattaga agaagttaaa gcaaaattgc acgacttagg tttaggttta
    21241 cgtaaagaag attaaaacct tttacgaagg agggaaacca acgtgagtta tcgtaaacta
    21301 ggacgcacat ctagccaacg taaagcgatg ttgcgtgatt taacaactga tttattaatc
    21361 aacgaacgta ttgtgactac tgaagctcgt gcgaaagaag ttcgctcaac tgcagaaaaa
    21421 atgatcactt taggtaaacg cggagatttg catgcacgtc gtcaagctgc ggctttcgta
    21481 cgtaatgaag ttgccagcgt gcgtgaagaa aatgaggaca tcgtcattga atcagcttta
    21541 caaaaactgt tcaacgatat tgcacctcgt tacgcagaac gtcaaggcgg atacacacgc
    21601 attttgaaga cagaaccaag acgcggtgat ggtgcaccaa tggttgtctt agaacttgtc
    21661 taaaatcaca ctaataacat cactttatag atgattcttt tagagtgtta tgatgttgga
    21721 agatattttt ccgagtctag ctcagcgctg ctcctcaagc gaatatcgct tggggagtaa
    21781 gcactcatca taaagtgttt ttttatacct tcaattacct gtcatgacag atatgtttga
    21841 aggcataaaa aaacatttta agcgagaaat aattgaaaat tttgtctttt tttgatagtg
    21901 ttagtaggaa ctttaggatg aagaaggagt aatcatggag ccaattattg aattagaaaa
    21961 gataaattat aaataccagc cagatgatct tcgccccgca ttgaaagatg tctcctttac
    22021 aattgataaa ggtgagtgga tcgctattat tggacacaat ggttcaggaa aatcgacact
    22081 tgccaaaacg atcaatggat tgcttctgcc agaatcaggg atcgtgaaag tgggaaatca
    22141 aatattggat gaagaaaata tttggacgat ccgacagatg gttggaatga tttttcaaaa
    22201 tccagacaat cagtttgttg gttcgacagt agaagatgac gttgcttttg gtcttgaaaa
    22261 tcaagggatt cccagagaag aaatgctagt gcgtgtgaaa gatgcacttg agaaagttag
    22321 aatggctgag tttgcttcac gcgagcctgc tcgtttatca ggagggcaaa aacaacgtgt
    22381 tgccattgcc ggcgtggttg ccttacgacc tgatatcatt attttagatg aagcaaccag
    22441 tatgctagat ccagaaggtc gtgaagaagt gatctctacg atcaagaaga tcaaagaaga
    22501 aagtcaattg accgttatat ctatcactca tgatattgat gaagcagcta atgccaatcg
    22561 gatactagtg atgagacaag gcgaacttgt ccgtgaaggt acaccaaaag aaattttttc
    22621 tgctggtcct gaactgatcg acttaggtct agatctacca tttcctgaaa aactgaagag
    22681 cgcactgaaa gaacgtgggg ttgatgtgcc cagtgaatat atgacagaag aaaggatggt
    22741 ggattggtta tggacatccg ttttgaacaa gtagacttca cttatcagcc gaatacacca
    22801 tttgaacaaa gagccttatt tgacatcaat atgacgatca aagaaaacag ttatactgca
    22861 ttagtagggc ataccggaag cgggaaatca accttacttc agcatttgaa tgcgcttgtt
    22921 aagccaacaa gcggtacagt ccatattgga gaacgagata ttcagccgga tacggataat
    22981 aagaatttaa agcccatccg aaaaaaagtt ggcatcgtct ttcagtttcc agaagcacag
    23041 ctattcgaag aaacggtagc aaaagacatt gctttcggtc ctaaaaactt tggtgtcagc
    23101 gaagaagaag cactagtcct agcaaaagaa acattagaac aagttgggct ggatgaaagc
    23161 tatttggaac gttcgccatt tgaactttca ggagggcaaa tgcgtagagt agcaatcgct
    23221 ggtgtgcttg ccatgaggcc ggaagtactt gtattggatg aaccaacggc aggacttgat
    23281 ccccagggca gaaaagaaat gatggagatg ttttggcgtc ttcataagga acagcagatc
    23341 actattgtat tagttacgca tttaatggat gatgtagcca attttgcaga ttatgtctat
    23401 gtgttggaaa agggacgaat cgttaatagc ggggagccgc aagaggtctt tcaaaacatt
    23461 gaatggctaa aagaaaaaca actaggcgta cctactgcca ccgagtttgc agaagaatta
    23521 atggctaaag ggatgaactt tgctaccttg cctttgacag ctgaagaatt agcagacgct
    23581 atcgtccatt atgctggagg aaatgcccat gatgaataaa ttgatatttg gtcgttatat
    23641 gcctggagat tccttgatcc atcgattgga tccccgagct aaattattag ccagttttta
    23701 ttttatcgga atcatttttt tagcaaataa ttgggaaacg taccttttgt tggcattttt
    23761 cacattactt tctgtcttct tgtcaaaagt agatttgaaa ttttttatcc gaggcattcg
    23821 cccattgatc tggctaatcc tcttcactgt agctttgcag gtcttgttta caagtggcgg
    23881 agaaacttat tggagttggg gtgttttcca tatcactgaa tttggtgtga tgaacggcct
    23941 ctttattttc tgtcgattcg tgttgatcat ttttatgtcg actctactca cgcttactac
    24001 accgcctttg gaattatcgg atgcgattga gtacttgctt cgtccattga aagccgtacg
    24061 gttcccagtc cacgaaattt cattgatgct gtcgattgct cttcgttttg tgcctacatt
    24121 gatggacgaa acagaaaaga tcatgaacgc tcagcgcgct cgcggtgttg attttggtga
    24181 aggaaaccta gttcagaaaa tgaaagcagt cattcctttg ctgattcctt tatttgtcag
    24241 cagcttcaac cgtgcggaag atcttgcgac tgctatggaa gcaagaggct acaaaggtgg
    24301 ggaaggaaga acgaaatatc gtgtattgca ttggcataat agagacacga tggttatcat
    24361 tgcttttgta ttattaactg tgctactgat ctttttaaga ggataaacgt tcagtgggcc
    24421 gctatctatt cttttcttgc tgatctgttt gcaagaaagg atgcaagcag tgtatgacaa
    24481 gaaatattag ggtctgtggc agactaaggt cacaggcctc tttcttttat ctgaaaagaa
    24541 agaacgaaaa aattttatag aaaaatcatt attccagata accttttagg aacaaaggag
    24601 agaaaaacat ggtacgttac aaagcaatca ttgcgtacga cggaacaaat tttaatggat
    24661 tccaaaaaca gccaaatggg cgtactgttc aagaagaagt agaaaaaaca ttacaaaaaa
    24721 tggcaaatgg aaaagaaatc acggtctttg gttcgggaag gacagatgcc ggagtacatg
    24781 cgatcgggca agtaatccat tttgattatc cagaggagcg tccgttagaa cgaatgcgtt
    24841 tcgcgctaga tacccagtca ccagaggata ttgctgtaag acaagtagaa attgtttcag
    24901 aagacttcca cgcaagatat ttggtcaaag aaaagacgta tcaatttcga gtggatatag
    24961 ggaaaccacg cagtcctttt cgacgccatt atgcaagtta ttttccttac ccacttgatt
    25021 tggaaaaaat tcagcgggcg cttcctgatt tattagggac tcacgatttc acctcttttt
    25081 gtgcatcagg aagctcgata gaggataaaa tacgtacgat ctacgaggct aagatggagg
    25141 taaatgaaac aaaagacgaa ttgttgttta catttagggg aaatggcttc ttgtataaga
    25201 tgatacggat cttagtagga acgcttttga aaattggcaa tggccgattg cctgaagata
    25261 gtatcccaga tattattgcg aaaaaagatc gtaatgcagc aggacctaca gcacaccctg
    25321 aaggacttta tttgtatgaa gtgaaatatg aataagaggt tatgatagaa gtggtcagct
    25381 tcaagaaata agaaggtatt tacgaaaatt acttctcaaa attttgcaaa atttcggctt
    25441 attttcgaag aagctacttc tggaacaccg tttaagaaag aggtcgtgag cctgtcattt
    25501 aactctgagt cacaaagagc aatttcaaaa aacagcttct catctttgat gaatatcgtg
    25561 acttgtggac gaggcgttga taagtgaatc atctttttgt tacctatttt ttctgtaacc
    25621 aaaaaacgat ggaacgttgt tttgattgcc cttggtgcgg acagtctgaa caggttccat
    25681 cgtttcttta ttttctacgt cttcttctgt gtgtgtttcg tggttgttct tctattgctg
    25741 gatcaatcgt gccataaaag gggcgattgc ggtcaaggta ccagacataa agaataaaca
    25801 atgcaataca taggacaaaa aggattgcac ctatcgtaaa tccttcaggc aagtaactga
    25861 aagtgacagt atgtttacct gctggaactt ttatcatcaa gaatgcgtct ttgaaattgc
    25921 tgaccttgac tggtttccca tcaattttag ctttccatcc tttgtcataa ggaatagttg
    25981 tcaccaacat ggaatcttca gcggtattta cagtcccaga agcttttctg ccagtggctt
    26041 ccatttcgac acctttttct ttgatctgat tcattgcggc ttgataggct acaacatcta
    26101 gaccaacgac tttaggctcc atgaaactta tttctttcgt cccataaaaa cttgcagtaa
    26161 atgtcactgt tgtctcttga ggataatagc caagatcata gtattgacca gaaatgttga
    26221 tttgcgtttt tcgcgatgta ccattgacag tgatcgtcgc actagaactt tctaattgcg
    26281 caaaattggt tgggaacaaa cttaagtaag cttgtgtatt tgcaggcaca tggacttccc
    26341 aagtgagctc ttttggctgg ttatctgctg tttctgagta agtgacgcca gtcgttgtct
    26401 ttgtgatcgt cgtattcaac tgattcttca aagtaggtgt atagaatgta aaatactgtt
    26461 catttagacc agacaatgta ttgatcaagt ttgtctgact ggtcaaatta tctgtcactg
    26521 gctgctcaac atcattaacg gacaacggtg cagtaaatgc tagtggcaat gcattgacat
    26581 tctgatacag tgtatagtcg ccgctttgac cagcttggcg gaagccatat ttactgaacg
    26641 tgctttcgtt tttagaaatg ttatatttga ttcctgtaaa cccatccatc agcaaagtat
    26701 tattgttata acggatattc aaatttgtcc ctcttgaacg gaaacctaat ttatccaggt
    26761 aagaggaaga attacgattt cttatagaag aaaacaaact gataccactg taaccatagt
    26821 tgatcgaatc gtttgcagat actggatcca gattttccaa gcggtaaaac gagtcggaat
    26881 ctttttttgt ttcatttaca agagctttca agtcaggata aggatcagta tataaactgc
    26941 gggaagcata attccaatca tcaagaatgc catggatcat actgttcgta ttgaccagtg
    27001 cttcagcaga catgatcaat aataacaata tactcagatg attgaatgaa agttttttat
    27061 gctgataaaa tcccacagca aaagcataaa tccctaagaa aaccactgtc aatacaaagg
    27121 aagtcatcgt cacataggtg tagcttcctt caggtttgat gccccatgcc accgcaaaga
    27181 gagcagctac gaccaaaatg attcctaaca acagcccacc atcgtcttgt tctatctttt
    27241 cccatccttt tcctgcaagc aggagcacta agaaagagaa cagataggca taacggaata
    27301 aaaacatatt cggtgcatgc ataccatgcc agaacaaatt caatggaaca agataaaaac
    27361 ttgcgataag tatggcgaaa agactgccgt acaaaaattt atttttcaac ggtactttcc
    27421 gactggcaaa ataaaacaga cagaggacta atggtagcag acctgcgtaa acgaacggaa
    27481 tcgatccgta ttttgtcgtg tcataaacac caatcatatt tttcatgatt agatccagat
    27541 aagaagtcgc ttctgttttc aacgtagtga tctgactcag tgcctcgcca tttgaccgaa
    27601 gatccagcac tgctggcaat acaatgatca ttgaagcgcc gcctgccagt aaagaagtga
    27661 taccataagg aacaatcgtt tttttatatt ctgaccagtt tgtcaataga cgggcgataa
    27721 aatataagaa agaaaatacg ccaatcataa aacccatata aaaactggat aaaaacaaga
    27781 gtaaataact gataaataaa agtttcggcc gtttaagatc cataacacga tgaatcccca
    27841 aaatcactaa tggaagataa acaaatgcat ccagccacat gatgatctcc gaatgtgcag
    27901 tgataaatga gatcaatgcg taactgacgc taagtgcaac atgcccccag cgaggaatct
    27961 tgaacgtacg tgcaagaaac caaaaagcca atccggcaca gccgacctta agcaaagtca
    28021 gaacatacag tgcatctggc atcatctggt ttggaaaaaa gaacaccagt ggcgtgaaga
    28081 tcccgcctag atagtaagaa attaaagata aataattcaa tcctagagaa gcattccatg
    28141 tataaaaaat actttgcttt ccaagaagaa catttcggaa gctggcatga aaattagaaa
    28201 actgtgaaaa ggcatcactt gccaaaatac ttctgctgct tccaggatag attccgatac
    28261 ttagataaac aagtgctaaa atcaaaaacg gaatcaggaa actagctgct gcgtagcgtc
    28321 cgttttctct aaagaatgac tttaaatttt ttttcatatt tattttccac caaatttcta
    28381 tgaagttgta ttcagtttat catatccttg tttttctctg cttaaaaaga gtctagtttt
    28441 cacaaaaaaa taaaatttag aataaataac ttcataattt gttcgtataa caaaaataaa
    28501 cgcaaaaaaa gggtaggaat caaaagtccc acccttaaaa atttttatta aataaaaaac
    28561 cgttcactat ctgaacggtc caagtatcat tcaattaaac gcgttcaact tttcctgatt
    28621 tcaaagcacg agttgacacc caaacttttt taggttttcc gtcgaccata acgcgaactt
    28681 tttgtaagtt aggtttaaca gtacgttttg ttgagttcat agcgtgtgaa cggttgtttc
    28741 cactttttgt cttgcgacct gtaaagtaac aaacttttgc catggtaatt tcctcctttg
    28801 ccttgatttg gagcaataga ataattcagc tcataccaga ctaacatatc atatagaatg
    28861 ggaatttgca agtacatttt catagataca attgacaatt gagaagaata attgtaaatt
    28921 aaacaaggtt atacctgaaa ttttgcaagg tttttgagta ctttgggtaa ctgcctttta
    28981 tttcaagttg cgctatgata aaatatgata gaagaaaaat gggagttttt tacccgttaa
    29041 ggaggctcat tcatggctgt aaaaattcaa acgtcagctg gaaccattga aattaccaat
    29101 gatgtaattg caacagttgt tggtggcgcc gcgacagatg tgttcggtgt tgtaggaatg
    29161 gccagcaaaa atcaaattaa agataatatt actgagattt tgcgtaaaga aaattactca
    29221 agaggagtag ttgtccgcca agaagataat ggtattgctg tagatgttta tacgattatc
    29281 agctatggta cgaaaatctc tgaagtttcc cgcaacgtcc aagaaaaagt gaaatacaat
    29341 ctagaggcat tattaggcgt aactgccaat tcagttaatg tctttgtaca aggtgttcgt
    29401 gtactgccag actaggcagg agactggctg gtaactcagc agtcgatctg aaggaggatt
    29461 atctaggtga gtgtaacaga aataagtgca agccagttcc aggaaatggt ccaggcaggt
    29521 gcgaatcgtc tgcaagtaaa tgcagaatac gtcaattcat taaatgtatt ccctgtgcca
    29581 gatggcgata cgggaacaaa tatgaatcta tctatgacta gtggagctaa ggcagtagta
    29641 gattcaacct cagaaaaagt aggagaatta gcagcaatgc tttcaaaagg attgctgatg
    29701 ggtgcgcgcg gaaattccgg cgttattttg tctcaattgt ttcgtggatt ttctaagcaa
    29761 attccagatg ttgtctcatt aaatgcttct gatcttgctg cagccttcac tcatggagtt
    29821 gaaactgcgt ataaagcagt aatgaaacca gtagaaggaa cgattttgac agtagctaga
    29881 gaagcagcga aagctggaga aaagaaagca aaaagtactg atgatgtcgt tgaagtaatg
    29941 acagctgttg taaaaggagc aaagcgtgct ttagctaaaa ctccagactt gcttccagtc
    30001 ctaaaagaag tcggtgtcgt agacagtgga gggcaaggac tgctgtttgt ttatgagggc
    30061 tttttaagtg cattgaatgg agaattccaa gcggatgaaa catatgaacc ttctccagca
    30121 gaaatggatg atatggtcaa tgcagaacat catcgcagtg tccaaggtca aatggcgaca
    30181 gaagatatcc attttggtta ctgtacagaa atcatggtaa aaatcggaga aggacctaca
    30241 gtcgatagta cattcgatta cgaagaattc cgtaactatt tagatggtat aggagattct
    30301 ttacttgtgg tcaatgatga cgagatcatc aaagtccacg tgcatacaga aaacccagga
    30361 gaagtcttga attatggaca aaaattcggt tcattagtca aaatcaaagt cgacaacatg
    30421 cgcttgcagc atgagacgat tttggaacac gatgaagaag tgaacgagtt tgcgaaaaaa
    30481 gaaataccac gtatcccgta tggtatcgta gcgatcgctg ctggtaaagg cgtccaagag
    30541 ctgtttgaaa gtttaggcgc taattatgtt atcagtggtg gacaaacaat gaatccaagt
    30601 acggaagaca ttgttaaagc gattcaagaa gtaaacgcag ataaagtgat tgtcttgcca
    30661 aacaataaga acatcttcat ggcagctgat caagcagcag aagtagcaga tatcccagta
    30721 gcagttgttc cttcaagaac gatttcccaa ggattgacag ctatgctttc attcaatgat
    30781 caagcttctt tagaagaaaa caaagaaacg atgacagaaa tgttgtctag cgttgtgagc
    30841 ggtcaagtca ctcacgcgat cagagatacc tcaattgacg gtgtagagat caaagaagga
    30901 gactttttgg gcatgatcga tggaaaaatc ctgatctcta acccagatat cacagcaaca
    30961 gcgttaagta cactagaaca aatgatcaat gaggacacag agatcgtaac gatcttggca
    31021 ggtgatgaag gaacagttga acaagcaaat gaatttgctg agaaactaac agaaaaacat
    31081 cctgatttgg aaatcgagat ccaccaagga gatcagcctg tctatcctta tctattttct
    31141 gctgaataat ttctgtgtca gaatatcttt ctaacacagt attcattcat aaaaggcctt
    31201 cggttagtga tcaggtatga tcgctgacca agtccttttt ttgttaaagg agggaggaaa
    31261 aatttatgcg ttctttagat gatccaatca ctatgctggc aggagtcgga gaaaaaagag
    31321 cagctagtct tgccagcttg ggcatccaaa cgattgaaga cttactgact tattatcctt
    31381 ttcgctacga agatattcag gaaagaaatc taaacgagat ccaggatcaa gaaaaggtta
    31441 ctttgaaagg aatcgtagtc tcgcctcctg tgatgaatcg atttggttat aaaaaaagcc
    31501 gattgcaatt tcgtatgatg caagaacatg atgtgttcaa cgtttctttt tttaatcaac
    31561 cttatctaaa agaaaaagtc attttatcgg aggaaatcgc cgtatacggt aaatgggatg
    31621 caaagcgtaa agccttgaac gggatgaaga ttttaggttc tcaaacaatg gatgactttt
    31681 cgccgatata tcacgtcaac aaatcaattc gtcaaacgac attagttgat ctaatcaaaa
    31741 aagggtttca agaatacggc acattgattg aagaaaatct gccaaacaac cttgtggaga
    31801 aatatcgact gcttgaccga gcttctgctg taagaagtat gcattttcca aaagaccacg
    31861 aagaaaacca tcaagccaaa cgacgtgtcg tttttgaaga gttcttttta tttcaaatgc
    31921 gtcttcaagg attgaaaaaa gcagaaaaag cagaaacgaa tggtttggag atcctttatg
    31981 atgtccaaaa attgaaacaa tttactcaaa agctaccttt tgaactgaca aatgcacaaa
    32041 aacgtgtaac gaatgaaatc tgtcgggatc ttcgcagtcc gcagcatatg cagcgacttt
    32101 tgcagggaga tgtaggtagc ggaaaaacag tggttgccgc gattgcgtta tacgctaccg
    32161 ttacagcagg tttccaaggt gcgttgatgg tgcctacaga aatcttggca caacaacata
    32221 tggaaagctt gcaacagctg tttgatccca atgaagtaac catcgcattg attacggggt
    32281 caacgaaagc aaaagaacga cgtgagctcc ttgaaaaact agagcaaggg gaaattaacg
    32341 tcgtgatcgg cacacatgca ttgatccaag atggagtcga atttcagaat ttaggtctag
    32401 ttattacgga tgagcagcac cgttttggcg tgaaccagcg gaaagtactt cgtgaaaaag
    32461 gctggcgacc agatgtctta tttatgacag cgactccgat tcctagaaca ttagctatta
    32521 cagcatatgg tgagatggat gtatctatca ttgatgaact tccagctgga cgtatcccga
    32581 tcgaaacacg ctgggtaaga acacctcaat tggacagtgt actggattgg acgaaaaaag
    32641 aactggcaag aggacatcaa atgtatgtca tctgtccgct gattgaagaa tcagaagcat
    32701 tagatgtaaa aaatgcagtg gaaatctatg aaaaacttcg tgaactattt gcaccaaaat
    32761 atgaagttgg cttgttacat ggtaaaatga agaatcagga aaaagaagta atcatggaag
    32821 ccttcaaaga caaccagatg cagatattag tttcgactac tgtaattgaa gtaggggtaa
    32881 acgtgccgaa cgcgaccgtc atgctgatca tggatgcgga tcgatttgga ttggcacagc
    32941 tgcaccaact tcgtggacgt gttggacgcg gaagtgatgc ttcctactgt atcttagttg
    33001 ctaatcctaa aaatgaacta ggtgtggaac ggatgaaaat catgacagag accaccaatg
    33061 gttttgtact aagcgaaaaa gatctggaat tacgtggtcc tggtgaagta tttggcttcc
    33121 gtcaatctgg tctgcctcaa tttgcgattg ctgacttggt gacggatgga aatgttttgg
    33181 aggtagccag agaagaggct gcagcccttt ggcaaataaa ggattggcag atgcttcctg
    33241 agtatcagcc tttagcgcaa agcctccaaa tagaaaatca ggataatcgt tatttcgatt
    33301 agcagactgt gctatacttt atactgatga cggaaaaagg agtggaaaca atgaaaattg
    33361 ccgtagatgc aatgggcgga gatcacgcac ctcaagcaat tgtagaaggc gtggctttag
    33421 cgcagcaaga ttttccagaa gtagaattct tactttacgg aaaagaagca gaaatcaaga
    33481 aatacttaac aaatgaaaaa aacatcacga ttatccatac agacgaaaaa atcaatagtg
    33541 atgatgagcc agtaaaagca attcgacgaa aaaagactgc atcaatggtc cttgctgcac
    33601 aagcagtgaa aaacggtgaa gcagatgctg tattttcagc agggaataca ggcgcgctac
    33661 tagcagcagg gttgttcatc gtaggacgta tcaaaaatat cgaacgccct ggcttgatgt
    33721 ctaccttgcc agtagtaggg aaagaaggtg ccggtttcga tatgctggat cttggcgcta
    33781 acgcagaaaa caaaccagaa catttgttgc aatacggtat tttaggttct ttttatgcac
    33841 gtaaagtccg aggtatcgga cgtcctcgtg tcgcactctt aaacaacgga acagaagcaa
    33901 caaaaggtag tgaagtgacg aaaaaagcct atgaactcct tcagaatgaa acaagcttga
    33961 attttattgg aaacgtagaa gctcgtgagc tactcaatgg tgtggctgat gtagtggtca
    34021 cagatggatt tacaggcaac gctgttttga aatctattga aggaacagca atgaatatga
    34081 tgaatctatt aaaatctgct attctaaatg aagggatcaa aggaaaaatg ggcgctatgc
    34141 ttttaaaaga cggcttacgt tctttgaaag cagaaatgga ttattccaaa catggcggtg
    34201 ccgttttgtt tggcttgaaa gcaccagtca ttaaaactca tggagcaaca ggccccgatg
    34261 ctgttcgcta tacgatccgc caaatccaca caatgcttga gacagatgta gttggacaat
    34321 tagtcgaaca atttgaaaaa agcgaagaat agtatttgga tatctgaagc gatccgttcc
    34381 attgaaaaaa aggtggacat tcctgaaaaa tgccagtaaa attagccatg gtgtaggtaa
    34441 ttgacaagtg tatgattctt ggaggtgaaa aagttgactc gcgaagaagt atttaacaaa
    34501 gtagcgaaaa ttatttcaaa tcattttgag atagataccg ataaagtgac ggatgaattg
    34561 aacatcaaag atgatttaaa agctgattca attagtatta tggaattcgt tttagagctt
    34621 gaagatgaat tcggtacgga aatttcagat gaagacgctg aacaaattga aacagtcggt
    34681 ggcgcagtag attatatcag cagccatctt aaataatttc aaataaaagc aaagaaggat
    34741 aaaaagctgc atgcagcttt ttatccttct ttttttaatc tttttgacac gtaaaagtca
    34801 tttttctgat aattctggcg ataaacagaa attatgttgc aaagtaaagc ggttttctat
    34861 ataattatta ataattcatt acacaaagat aagaataacc tttgttataa aacgatttat
    34921 tctgtgtaat gtcctttttt attaagaaaa acttccattt aggaagcaga gaggggtatt
    34981 cgattgtccg ataaccaatt attagaagtt cagaacctac atacaggatt ccgactcaaa
    35041 gatgactatt acgatgcagt agatgacgtt tcatttactt tagacaaaaa cgagatttta
    35101 gcaatcgttg gggaatctgg gtgcgggaaa agtaccttgg ctactacgat tatgggatta
    35161 cttgatccta ataacacaaa aattacaggt gagattttat ataacgatct gaatttaatc
    35221 gacctgaacg aaacgctcta taataaaatc cgcggaaatg atatcggaat gattttccaa
    35281 gatccgttgt ctgcattaaa tcctctcatg agaatcgaag accagatcaa agaaggactt
    35341 tcctatcaca caaagatgaa tgcagaacag cgtcaagctc gtgcactaga attgctagaa
    35401 caagtaggga ttcctaatcc tgcgagagtt ggacgccaat atccccatga actatcaggc
    35461 ggtatgagac aacgtgtcat catcgcaatc gcaatcgcct gcaaacctcc aatcgtcatt
    35521 gcagatgaac cgacgacagc cctagatgta accatccaag cacagattct tgatttactg
    35581 aaagaccttc aggaagaaac aaaaacaggg atcatcttga tcactcatga cttaggagtc
    35641 gtagcagaaa tggctgatcg agtggcagtg atgtatggcg gacagttcgt tgaagtagca
    35701 ggtgtaaaag aactgtttga tcatcctaag catccttata cacgctcttt actgaactct
    35761 ataccacaag aaggaaatca tgatgcagaa ctccatgtga tcgaaggtgt cgttccttct
    35821 ctgaaaaaca tgcctcgtaa aggttgtcgg tttgcaccac gtattccatg gataccagca
    35881 agcgatcatg aagaagagcc tatccttcat gaagtcgcac cgaaccactt ggtacgttgc
    35941 tcctgctata agcatttcca ctttagagac gaaaaaggag aggtgtagat gacagaatta
    36001 atcacaatca aagacctgaa agttcattac cctattcgca gtggattctt caatcgagta
    36061 acggatcatg tatatgctgt agatggtgtg gattttatta tcgaaaaagg aaagacatat
    36121 ggtttagtgg gagagtctgg ctctggaaaa tctactacgg gaaaagccgt tgtgggatta
    36181 gaaaaggtaa cagccggaca aatcatgtat gaaggaaaag atgtaacgaa gcgaagcaac
    36241 cgcaaaaaaa tgggttataa caaggatgtg cagatgatct tccaagattc aatgtccagt
    36301 ttgaatccta aaaagcgagt gttggatatc attgctgaac ccatccgaaa ttttgaacgg
    36361 ttaagcgatc aagaagaaaa gaaaaaggta aaaggactgc tagatatcgt tgggatgccg
    36421 gaagatgcgc tatataaata tcctcatgaa ttttcaggcg gacaacgtca acgtctagga
    36481 gtagcccgtg ctgttgctac caatccaaaa ctgatcgtag ccgatgaacc tgtatctgct
    36541 ctggatctat ctgttcaagc gcaagtattg aatttcatga aacgaatcca gcaagaatac
    36601 ggcctcagct atttattcat ttcacatgat ttaggtgtag taaaacacat gtgcgacaat
    36661 atcgcaatta tgtacaaagg tcgatttgta gaaatcggta caagagaaga catttataat
    36721 gacccgcgac atatctatac gaaacgtctg ctttcagcta ttccgcaaat cgatgtagaa
    36781 aatagggaaa gccacaaaga aaatcgacga agagtagaac aagagtatat ccaaaaccaa
    36841 aaagaatact atgatgcgac tggtcgagtc tacgatttgc gtacgatcac aactacacac
    36901 aaagtggcgt tgaaagatgg aggtgcaagc taatgtggaa aacgattctg cgacgtgttt
    36961 tattgatgat cccgcaagtt attattttaa gtgttctgat cttcttatta gcccaagcga
    37021 tgccggggga tccatttacc ggtttgatca atccaaatca ggatccggca gtgatcgaac
    37081 agatgcgaca agcagctggc ttgaatgacc catggtatgt ccagtacttc cgttggatcg
    37141 gaaatgcttt gcaaggcgat tttggtcaaa gtttcttata caaacgctct gttgctgact
    37201 tgatcggcga acggatcatc aacactttat atctatcgat cttgacagtt gtccttactt
    37261 atttgattgc tgtgcctctg gggatgcttg ctggccggta tcaaaattca atcttggata
    37321 agattgtcgt aatctacaac ttctttagtt ttgctgttcc tttatttatc ttcggcttga
    37381 tcatgctatt tgtttttggt tatcgtttag gctggttccc aacaagtgga tcacaaacac
    37441 taggtttgca aggtggattt atcactcaat ggatagaccg tttgaaatat attctgttac
    37501 catcaattac ccaagcattt ttggcgactg ccgtcacaat ccaatatctg cgaagtgaag
    37561 taatcgattc gaaatcgctc gattttgtca gaacagcccg ttccaaaggg gtaccgacga
    37621 ataaagtatt cagtcgacat attttccgaa atgcagcgtt gccgatggct tctcaaatgg
    37681 ggtatgaaat cacttcgttg attgctggtt ctgtagttat cgaaaagatt tttggttatc
    37741 cgggagtcgg aaaactattt attgattcca tcggccaacg ggactatacc gtgatcacat
    37801 ccttagtatt gattttagga atcgcgacgc tagtggggac attactttca gatatcatca
    37861 tgagtatcgt tgacccgcgt attcggattc aataaaacca atcagaaagg agaatggatc
    37921 gatgagtgaa aaaaacaata cagcagttca agaaagtatt cctcctatgg ggatacgaat
    37981 gattgctcga gaatttaaaa aagataaaat tgctattttt tcattaggtt tgctagtggt
    38041 tttattatta gtgatcttta tcggtgcgat gctgaccgat caagacaaag tgatgtatgt
    38101 tagtatcttc gacaaatacg ctgcaccagg aacagtttcg acacaaggac aaaagttcat
    38161 tctgggagca gatgaaggcg gacgtgatgt attaggacaa ttgatcatag gtgctagaaa
    38221 ctctgttacg atcggatttg cgatcacgat ccttacttcg atcatcgggg ttggattagg
    38281 gatcatctct ggctactacg gagggatcat tgacaacatc ttgatgcgga tcgttgattt
    38341 tatcatgatt ctaccgatca tgctgatcat tatcgtcttc gtatcgatta tttccagata
    38401 caatgtctgg tcctttatat tcatcatgag tgcattttat tgggtagcta aagcccgatt
    38461 attccgaagc aagacattat ctgaagcaag aagagattat gtcagtgcct caaaaacaat
    38521 gggaaccaat gattttaaaa tcatgttccg cgaaatcatg ccgaatctaa gttcattaat
    38581 cattactaat ttgacgatga actttgcggc aaacatcggt atcgagacaa ctttgacttt
    38641 cttaggtttc ggcttgccgg caaatgtacc tagtttaggt acattgatcg gttatgcaag
    38701 taacggagaa gtcttagtga atcggcaatg gatctggtta cccgcatcaa tccttatttt
    38761 agttttgatg ttgagtatca attatgttgg acaagcattc aagcgttctg cagatgcgcg
    38821 ccaacggtta ggctagtata gaagcgaagg gggaaatagg aatgaagaaa acgatttttg
    38881 gatttgcagc gctgatgtct gctgtcgttc ttgcagcatg cgggggcgga ggtacagata
    38941 cctctggaaa caactctgga ggcaacggca ataacaaaga ggaattaact tttccaattg
    39001 caacaaaaaa taatgatgat gcaatcgaag acggcgaatt agaagctgcg gtcgcaacgg
    39061 acagtcagtt taaaggattg ttccagtggg agttttacca agattcatat gatgcagctt
    39121 ttatggctcc ttctcatgaa ccgctatttg caaacgatga cgatttcaga atcacagacg
    39181 acggagctgc aacgcttgat ctagatgaag atacgaaaaa agcgacaatc aaagtaaaag
    39241 acaacgtcaa atggtcagat ggtgaagatg taacggcgga cgatgtgatt ttcccttacg
    39301 aaatcattgg gaataaagat tacactggta tccgttatga cgacactttc cgtaacatcg
    39361 taggtatgga agaatatcat gatggaagtg ccgacacaat ctccggaatc aaaaaaatag
    39421 acgacaaaac agttgaaatc gaatataaag aagtaaaccc aagcatgctt caatcgggtg
    39481 gtggtatctg gtcatatgca gcaccaaaac atacgttaga aggtgtagca atcaaagata
    39541 tggaatcaag cgatcaagta cgtaaaaatc cagtgacttt tggcccttat tacatgagta
    39601 atatcgttgc aggagaatca gtagaatact tgccaaacga gcactactgg aatggtacgc
    39661 ctaaattgaa aaagatcacg atgaaatctg taccgatggc aagtgcaact gaagcattga
    39721 actctaaatt atatgatatg attttccaaa tgccaacaga tacttacgat acctataaag
    39781 aaattgctgg ctatacaaac ttaggccgtg aacaaacttc ttatacttat ctaggattca
    39841 agttaggtaa atggaatgca gatacaaaga gtgtggaata caatccagat tcaaaaatgg
    39901 ctgataaatc attacgtcaa gcaatgggct atgcattaga taataatgca gtaggtgaac
    39961 gtttctatgc aggtttgaga agcaatgcga cttcattgat tcctccagta ttccagtcat
    40021 accatgatga tgagatgaaa ggattcacac aagatctaga caaagcgaaa aaactattag
    40081 atgatgctgg ctacaaagat gtggatgacg atggtttaag agaagacaaa gatggaaata
    40141 aactaacaat caactttgct tcgatgtccg gtggggaaac agcgcaacca ttggcagatt
    40201 actatatcca acaatggaaa gaaattggtt tggatgtcca atacacaaca ggtcgtctga
    40261 tcgaattcaa ttctttctat gataaattag aaaacgatga tcctgaaatt gatgtttacc
    40321 aagctgcttg gaatacagga actgatccaa atccaagtgg actatgggga ccaaatgctg
    40381 cgttcaacta cactcgttat gaatcagaag aaaatacaaa attgatcaac gatatcagtt
    40441 caagcaaagc atttgatgaa gagtaccaaa aagaagcata tcaaaaatgg caacaatatg
    40501 catttgaaga agcatttgcg attccaacgt tgttccgtaa tgaagtattg ccagtcaacg
    40561 accgtgtcaa agactgggat tggtcttatg atgtaccaaa cccatgggca aatgtttctg
    40621 ttacagcaga ttcacgttca taatcactaa aaatgaccga aaaagggcga tgtccttttt
    40681 cggttttttg atatttttct gtttatttct ctaatattca ggtcaaggcg ctgatttcat
    40741 gacttctcca ataaacggtg ttcaaagtcc agtttctcgg tattaagtca aatttatgca
    40801 aaaatatgga aagccatttt cacaaaattt tttcttcata ctcgaaacta aacgaatttt
    40861 tcacaacttc tcgtcaatca ctaggattat taaagcttta cttacctttt gaccgaacca
    40921 tttcaaattt gacaaaaaac cagtataata aggaagtatc gaaaaaatat ccataaaagg
    40981 aatgtggaaa atggacaacc agctgacaaa agaactaaaa gaaaactata acatcgtttt
    41041 tcacgatcta aatttgcttg aacaagcttt tacccattca tcctatgtga atgagcatcg
    41101 caatctgcaa ttatcggata atgagcgact tgaattttta ggagatgccg ttttagaact
    41161 gatggtttct cagtatttat tccgtttata tcctcatttg ccagaaggaa aattgacaaa
    41221 aacacgagct gcgatcgttc gcgaagacag cttgtcgaaa tttgccaaag aatgtcactt
    41281 tgatcaatat gttttgttgg gcaagggaga agagaattca ggaggacgta cgcgaccagc
    41341 attgctttgc gacctattcg aagctttttt aggcgcgtta tatcttgacc agggattcga
    41401 cacgacccat gcattcatcg aaaaagtgat ttttcctaaa gtcaaagcag gtgctttttc
    41461 acatgagatg gatcataaaa caaaactaca agaagtcttg caaaaatcag gtgatgtatc
    41521 aatcgaatac cgattgatca atgaagaagg tccagctcat gaacgagtat tttggatcga
    41581 agtttatgta gatgatcaat tgattggcac tggacaaggg aaatcgaaaa aattagcaga
    41641 acaagctgcg gcggaaaatg cattggcagc actgtaaaag taagtgaggg aaagctttgt
    41701 atttgaaacg aattgaaata gccggtttta aatcatttgc agacaagaca gtcattgatt
    41761 ttgaaaatag cgtcacagca gtggttggac caaatggcag cggaaaaagc aatattacag
    41821 aggctatccg ctgggtatta ggagaacaat ctgctaaaag tctgcgaggc ggaaaaatgc
    41881 cggatatcat ctttgccggt tccgataccc gtaagcagct gaatattgca gaagtcacag
    41941 tgattttaga caatagtgat cattatctgc cattggagta caatgagatc agtgtaaccc
    42001 gccgttatcg acggactggg gaaagcgagt ttttcatcaa taaacaaagc tgcagattaa
    42061 aagatatcca agaattgttc ttagattctg gattaggaaa agaatcgttc tcaatcattt
    42121 ctcaaggaaa agtagaagcg attttcagta gtaaaccgga agaccgtcgt gggatctttg
    42181 aagaagcagc tggtgtcttg aaatacaagc agcggaaaaa gaaagcagaa cagaagcttt
    42241 ttgaaacaga agacaacctc agccgcgtcc aagacatcat ccacgaattg gaagaacagt
    42301 taactccttt ggccgcccag agtgaggcag caaaggagtt tttacggcta aaagaaacac
    42361 tgactcaaac cgatgtctct ttgatggttg ctgaaatcaa gacagcaaaa aaagattggg
    42421 acaacaaaca agcacagctt gcgaaattca atctagaatt aggcaaacta tctgagtcga
    42481 tccaagaaca agaatccatt ttggcgaagc agcgtaaaga aaatgcgcaa gccgatcgtc
    42541 tgatcgaaaa aaatcagcaa gtattattag atctttcgga aaaactgaag cagacagaag
    42601 gacaaaaaga tgttttgcag gagcggacaa agcatacaca aaaaagctcg caagaatatc
    42661 aaacttcatt agcagaagca caaaaaaagg ttaaacattt tgaaaagctt caagaaagct
    42721 tgatgaaagc agcagcagaa aaagaaacag agatccaaaa agctgaagca aatctcataa
    42781 aaacacagca agaattagaa aaataccaaa aatcaacgaa agaattattg gcagaattaa
    42841 gagatcaata tgttgatttg atgcaagaac aagcagctgt aggaaacgaa ctgaagtatc
    42901 tagaacgtca atatatacaa gaaacagcaa aaagcaagca gacgttagcc aagcaatcgg
    42961 aagtagaagc atcagtagac cgattgatct tacaaaaaga agaattgaca caaaagcagg
    43021 cgcagctaaa atcatcttta actgaaacaa aagagaagtt agaaatgatc caacaaaacg
    43081 ggaaaaagtt ccaagaagca ttagcaaaag aacagcctaa aatgtatcaa ttaatgaatc
    43141 aagtccagca gctacgtgct cgtaagaaaa gccttcaaga aatccaagaa aactattttg
    43201 gtttttacca aggggttcga ttggtcttgc agcataaaca gcagctttcg ggtattgtcg
    43261 gagcagtggc tgaactgatc gatgttcctg cagatttcac gttagctatc gaaacagctt
    43321 taggtggggc agcccagcat gtcatcgttg aaaatgaaaa agatgctcgg caagccatca
    43381 cgtatttgaa acagcaaaga ggaggaagag caacattcct tccactgacg acaatcaaac
    43441 caagacaatt accggctcat atattgacac aagcagcagc tgtagaaggg tttataggga
    43501 ttgccagtga acaagtcagt tatcccgatc aaatccaaac tgtggttcaa aacctgctag
    43561 gaacgatcct tttagccaaa gacttaacta gtgccaatgc aatcgcgcaa acgatccgct
    43621 atcaatatcg agtggtttcc ttagaaggtg atgtgatgaa tgccggagga tccatgacag
    43681 gtggagcgaa taaacgagga aaccaaggca gtttgtttgt acaaaatcaa gaattgaaac
    43741 agttgactag tgaatttgaa gaagcagata aacaactcca agcacaagaa aaaaaagttc
    43801 aggagctgca acaagaaaca gctcgtttag ccgaagaaca agaagttttg cgtacacgag
    43861 gcgaacaatt acgttttgaa gaacaagaag cgacaaatca gctacaaaac atcataaatg
    43921 aacttgaacg ttttgaaaaa gaaaagcaaa tctcaacttt tgagacacgt gaactgcagc
    43981 agtttatcga agattacgaa aagcaaacga acgaattgaa agacaaacaa acagatcttg
    44041 aatcgcaacg acaaaaaatc gacgaagaaa tcaaatcgtt aagtcaagaa agcgaccaga
    44101 tggaagccag aagagcacaa gtacaaagtc aaaaagcaca agaacaagca gatcttgctg
    44161 tcttgaaaga gcagtttaat catctgcaga tccagctccg cggtgcccga gtccaaaaag
    44221 cagaggcaac cgaacgtcaa gaagcgatag aaaagcagtt ggcgacattg acagcagact
    44281 tttctgatca tgaagtcaca gaagaaagtt tggaaaaaca aattaacgaa ttatctgctc
    44341 agcgagaaac attaaaagcc gagttggcaa aagccaaaga gcagcgagat agaaggcaaa
    44401 aggagatcga taagcttgaa gcagtcttgg cagagcgtaa tcgtgagcaa aaagcacgac
    44461 tgtcggaaca atccaaatta gaagtccaaa aagaccgagc tgaaatgatt ttagataatc
    44521 atttgaccta tctgcagtca gaatatcagc tgacatttga gaaagcttcc caagactatc
    44581 aagaaacaac cgatatcgaa gacagtagaa caaaggtttc atccttaaaa gaacagattg
    44641 aaaaactggg accagtaaac ttaaattcaa tcgaacaata cgaacaagtc agtgaaagac
    44701 atacattctt agcaacacag cgagatgatc tgttagcagc gaaaaaccaa ttgtttgaaa
    44761 cgatggatga gatggatgat gaagtgcgga caagattcaa agaagtattt gaagctatcc
    44821 gtcaagaatt caaaattgtc tttccaaata tgtttggcgg cggtaaagct gaattagtac
    44881 tgacggaacc aactgatttg ctgaaaacag gtattgagat tgaagtacaa cctcctggca
    44941 aaaaactgca aagtctaagt ttgctttctg gaggagaacg agcacttaca gccatcgcgt
    45001 tgctcttttc cattatccgt gtctgtcctg ttccgttttg tatcttagat gaggtagaag
    45061 cagcgttaga tgaagccaac gtgaaacgtt ttggtcgtta cttgagcgat ttccaagatg
    45121 acacacaatt tatcgtagtc acacaccgca aaggaacgat ggtcgcagca gatgtactgt
    45181 acggagtgac gatgcaggaa tccggtgtat caaaaatcgt ttctgtacgg atggaagata
    45241 tcaatgaaga ggcgaaaatc aacgaaacga aagtgtagga acgaaaatga tcaaattagt
    45301 agcaatcgat ttggacggta cattattaaa tgaacaaaag ctgatttcag atgagaataa
    45361 acaagcatta gcacaagcaa aacagcaagg ggtcaagatc gttctatgta caggacgccc
    45421 cttagctgcg atggcgcatt atctgcaaga attaggactg gtagatgaag gtgactacag
    45481 catcactttc aacggcggtt tggtacaaaa aaatgatact ggtgaaatca tggaaaagaa
    45541 agtgatggaa gtatcagata tccaacgctt atacaaactg gctcagcaat tggatctgcc
    45601 actagatgtt ttgtctgaca atgtggtttt acagctgcct agtgccccaa ggaagcaatc
    45661 cttgtacaat gtgttgaata agctgctgca attccaacca gcggatataa gcgatattac
    45721 agatgaattt gtgcttaata aggcagtaat cgcttatgac caagaagaat tagatcctaa
    45781 aatcaaagaa ataccagcag aattccatga aatgtatgag atcatcaaaa caagaagtat
    45841 gcttttggaa tttatgccaa aaggtgttac aaaagcttat ggtatttctt tattggcaaa
    45901 agatcttggt ttgaaacaag aagaaatcat ggcgatcggt gatgaggaaa atgatttacc
    45961 gatgatccaa tatgctggac taggcgtagc gatggagaat gctgtgcctt tcgtcaaaga
    46021 agcagcagat ttcgtgacag ccagcaatga agaaaatggc gtagccaaag tgatccaaaa
    46081 atttgtatta gagtaggggg agtcaaatgg gattttttga taaaatcaag cgtgctttta
    46141 ctggcgaaga gaaaacagaa gaagttgaag tacaggagaa gtacgacaag ggtttagaaa
    46201 agacgcgtaa aacatttggt gaacggatga atgagttgtt tgccaatttt cgaacagtcg
    46261 atgaagactt ttttgaagaa ttagaagaaa cacttatcgg tgcagatgta ggttttgaaa
    46321 cagcgatacg aattaccgaa gcattacgtc aagaagtgaa actcagaaat gcgaaaaaat
    46381 cggcagaagt ccaaaatgcg atcatcgaga aacttgtgga tctctatgaa gcggaaggaa
    46441 tcaatgaagt aaatgaacta aatatccaag aagacggact aacagttatt ttgtttgtcg
    46501 gtgttaatgg tgtagggaaa acaacaagta ttggtaaact tgcccatcag ttcaaaaacg
    46561 aaggtaaaaa agtattgatg gcagcagcag ataccttccg cgctggagca atcgaccagt
    46621 tagttgtctg gggcgaacgc gctggtgttg aagttgttcg tggcaatgca ggaggcgatc
    46681 cagcagctgt cgtattcgat gctttggaaa gagcaaagac agagcaggca gatgttttac
    46741 tagttgatac tgcaggccga ctgcaaaata aagtgaactt gatgaacgaa ttagaaaaaa
    46801 taaaacgagt aatccagcgg gaattaccag aagcacccca agaaacactt cttgtattag
    46861 atgcaaccac tggtcaaaac gcgatggttc aagcaaaaca attcaaagaa acaacagatg
    46921 tgaccggtct tgttttgaca aaattagatg gtacagcaaa gggcggtatc gtacttgcta
    46981 tccgtaatga attgcattta ccagtgaaat tggtcggtct tggtgaagga atcgatgact
    47041 tggagccgtt tgatccaaat gattttgtcg ttggattgtt caaaggatta ctaaaagaag
    47101 aataacccat gtctaaaaag caggagctgt gacaaaagtt gcagctcctt gtttgaatga
    47161 ttgtttttaa catagctgtg tttttgatgc cttcattttt atacttattt gtaagcggtt
    47221 ctcaaacaat ttcgttcttt ttcttagact ttttgattat aattagattc atatgaattg
    47281 gaggtcactt aatgatgaat tttaagcaat tattattgca tgtcaatgcg cgccctttta
    47341 tcgaccaagc tcgatttggt atagaacggg aaggacagcg ggttgatctt gcaggaaatc
    47401 tagcaaaaac cgatcatcca gcaatctttg gcgatcgatc ctatcacccc tatatccaaa
    47461 cagattttag cgaaacacaa acagagatga tcacccctgt taccgattct attcccgaat
    47521 tatttcagta tctcgctgct gtttatgatg tgactgctcg ttctataccg aaagaagaga
    47581 tgatctggcc attaagtatg ccacctgcct taccggaaaa agacgaagag atcattattg
    47641 caaaattaaa aaatttcgaa gatgtcttgt atcgacgtta tttagcaaaa gaatacggga
    47701 agcgaaaaca gatggtaagc ggtattcatt tcaattttga atttggcgat gagttgctaa
    47761 gaacattgtt cagccatcag gaagaatttc aagatttttc cgaattcaaa acagaacttt
    47821 atttgaaaac agcgagaaac tttatgcgct atcgctggat gatcacttat ttattcggtg
    47881 cttcaccaat gagtgagaag aactactttt tagatgaatc acatccgcaa gaacctgttc
    47941 gcagtattcg caacagtgcg ctgggctata cgaatcatcc aaatgtgaaa gtttcctatg
    48001 cttctatgaa acagtattta gcagatatcg agcgaatgat cgaagaaggg aaactttcgg
    48061 aggagaaaga attttataca ccgcttcgtt tccgaggcgg aaagaaagtc gcagatttag
    48121 caacaacagg tgttcgttat attgaattgc ggaatatcga tttgaatcct tatgcaagat
    48181 tgggtatcaa tccagagcaa gttcggttct tacaattatt cctgatgtac atgttatgga
    48241 cagaagaaaa agaagactgt gaccaatggg tagcagaagg tacgactcga aataacaaag
    48301 tagcactaga acaaccatca gatcaaacag aatttcatca agaaggtaga gaaattcttg
    48361 aaggaatgaa gcaaatgctg gttgaattgg attggttgga ttctctttat ttagtagagg
    48421 aagcattgac tcaaatggat catcctgaac aaacgttagc agcgaaactc tatcaagaag
    48481 cacagctatc gagtcagcaa gaagttgctg tggcattggg acatcaatat tataaagaaa
    48541 gccatgaacg tccctatcag ttggccggtt ttcgcgaaat ggaactttct actcagatat
    48601 ttatgttcga tgccatccaa aaaggtgtcc aagtcaaagt attagatgag tcggatcaat
    48661 ttttgcgtct gcaattccaa gaccatgtag aatatgtgaa aaatgcaaac atgacaagta
    48721 aggacagcta tatcgtccca cttatcatgg aaaacaaaac agtaacgaaa aaagtcttaa
    48781 aagaagctgg gttccgggta ccaggaggcg cagaattttc atccatggaa gaagcggtaa
    48841 aagcttatcc gagatttgcc gagcaggcat ttgtgatcaa acctaaatca accaattacg
    48901 ggttaggtat cacgattttc aaggaaggag ctagtctgga agattatcaa gcaggtttag
    48961 ccattgcttt tcgtgaagac agttcggttt tagtagaaga atttatgccg ggaacggaat
    49021 atcgtttctt tgtgatcgac ggagaagtcc aagccatcat gttgcgagtc cctgctaatg
    49081 ttattggtga cagtatccga acagtgaaag aacttgttga agaaaaaaat agtgaccctt
    49141 tgcgtggaac gaatcatcgt gcacctttag aattgatcca attaggtgaa ttagaacaat
    49201 tgatgttgaa agaacaaggg ttgacgatcg aatcagttcc ccaagccaat caaattgttt
    49261 atctaaggga gaactcaaat atcagtacag gtggtgattc gatcgatatg actgatgaat
    49321 tttctgaagc ttacaaaaaa atcgccgttt ctgcggtaga agcactagga gctaagatta
    49381 gtggtatcga tttgattata ccagataaag aaatcgatcc tacaactgat aaaaaagcat
    49441 atgggatcat cgaagcaaat ttcaatccag ccatgcatat gcatgtttat ccttttgcag
    49501 gaaaaggcag aagattgacg atgaatgtat tgaaattgtt atatcctgaa gtattttaaa
    49561 agaaaaagct tttgacagat tgtcctattt gatgtcaatc tgatcaaaag ctttttttgc
    49621 atgtaaaaat ttatgtgttt tttcgtttta aaataaacga tcccagaagc tttttttctt
    49681 ttttggtttt tctttttcag ctggcgaaga gttttcttct gggtattttt tttcgatatc
    49741 aatcacaggt tcatttactg cttcttttgc tgttaagagc aatccgaatt cctcaggatt
    49801 tgcagtgacg taatcgttga ctacagtgaa acggctattg tttttcgttg ctagctggat
    49861 atattggttt tggacagaaa ggggtagagc cccattcaag agtatggaag catctggatg
    49921 ttttttgagt tcttttaaaa agttttcttt gttttttggt tggatcattt cagcaacagt
    49981 catactaaga tagcagcgtt ccctgaatgt ccctaaatat ttatgctgtt cttcagggtt
    50041 cactaaaggt gtaccataaa gtcccttatc caaatggctt tttacatcat cttgtgccat
    50101 aaaaaatacc ctccttcttc tctttgtctt tcttacgatt atagcatatt cccaataata
    50161 cctaaaacat atgaaaaagc ggttgctttt gttcaacgat tcacttaaaa taagaatgaa
    50221 aatcattctc aattagaaag gtggattgga tgcgctacgg acgactagta gcattattag
    50281 ttattttaag tagtatttca ttgttcatcg gagtatcgaa tgtacaattg tcagaactgc
    50341 tccagttaga tgggcaaagc ttacaaatct tgctagtgac tcgtttgcca cgaacgattt
    50401 gtttgatcct tgtgggagcg accagtagta tttgtgggtt gatcatgcaa catttgaccc
    50461 aaaataaatt tgtttcacca acgactgcgg gcacgatgga tagtgcgcgt ttaggtattt
    50521 tagtggctat gattttccta cctggtgcat ccttgattgt ccgatccttg acagcatttt
    50581 gctttgcttt tgcaggaaca ttgctttttt tatcccttac gcgattgttc ctccagaaaa
    50641 atcaattgat gcttccgttg attggagtca tgttagggaa tatcattggt tcagttgcta
    50701 ctttttttgc ttatcaattc cagttggtac aaaatatgac ttcatggctt caagggaatt
    50761 ttgcgactgt gatacgagga aattatgagt tattgtatgc tgttgttcca ttgttgctct
    50821 tggtttggct atttgcttac cgatttacga tcattggtct gggcgaatcg tttgcagtaa
    50881 atctgggaat agattaccag aagatgcagt ttttaggcat tggcttagta tcattggcta
    50941 gtgcagtcat gttgatcatg gtaggaagca ttccttttat cggagtgatc attccgaatc
    51001 ttgtctctag aatatatgga gatcaagtgc acaaaacaat tggcgtaact gctttagcag
    51061 gtagtttgtt cttagtattt tgcgatcttg tcgctcgcgt gttgattttt ccttatgaaa
    51121 taccagtcag tgtgatcgtt ggaatcatcg gtggggctat cttcctttat cttctgataa
    51181 ggggacgacg atatgcttaa aaaaaagcca tctattttta ttggtatgct tgttcttgtt
    51241 ttatttgtta caggaatgta cttgacgatc catacatatg gaaactggca ttttgcatgt
    51301 gcattgagga gtaagaaaat ttttgcattt atccttgtct cgctagctgt cagcagtgcc
    51361 acaatcagct ttcagacaat tacgcataac cagtttttga ctccttcagt attgggactt
    51421 gagtcgttat atgtccttgt ccaaacggtc cttttcttct ttatcggagg agtgacgatg
    51481 cttcagcaga caggtacagt ttttttctta ttgaacgttg ggctgatggt cgggctgagt
    51541 ctattgttat ttttctttct tttgaaaaaa atgagagcga atcttttttt attactgatg
    51601 tcagggatga ttttaggtac gctttttggt agtgtcagta cctttttaca agtcatcatg
    51661 gatccgaatg aatatgattt gcttcaaggc agattatttg caagctttgg caatatccag
    51721 agtcagtatc ttttgcctgc ttctttgatc attatcggct gctggtacct gttatttcga
    51781 tatcgacatg tgctagacgt tttccatctc ggaaatgaac aagcagtaaa cctcgggata
    51841 gacacagatc gttttgaatt tttgatttta tgttgtgtca gtcttttgac agggacatca
    51901 acggctcttg tgggtccagt tactttttta gggtttattg tcgcaaatat cagttatcgt
    51961 gtcttttcta cttatcgtca tggtcagcta tttatcggaa gctttctggt agctatgctg
    52021 tttctgacag gaggacagct cgcagtagag cagattttcg gctggaatac aacagtgagt
    52081 gtcgtcgttg aatttgtcgg aggaatttat tttgtcacaa aattgattcg cgaaaggaag
    52141 agtatcctat gattgattta caaaaggtca ataaactgta ccatggaaaa tcagtggtta
    52201 aagatgtaca gttaacgatt cctgaaaaga aattgactgc ttttatcggt ccaaatggtg
    52261 ctggaaagtc aacggtgttg tcgatgatca gtcgtttgat accaaaagat acaggtgaaa
    52321 tctatttaga ccataacgaa gtaaaggctt ggaaatccaa tgaattagcc aaaaaattat
    52381 cgatcctacg acaatcgaat gaaataaatg tccaaatcac cgtgaaagaa ttagtagaat
    52441 ttggacgatt tccttatacg aaaggaaaat tgacgacaaa agacgaggaa atcgttcagg
    52501 aatcactgga acaccttggg ttagaagaaa tagcggatca atcgattgac actctttcag
    52561 gcggtcaact ccaacgtgcg tatattgcaa tggtgctagc gcaagacacg gattatattt
    52621 tactggatga acctttgaac aatctagata tgaattttgc ggttcaaatc atgcagattt
    52681 taaaaaattt agtacaagaa atcgggaaaa caattgttat tgttcttcat gatatcaact
    52741 ttgctgcaag ttatgcagac gaaattgttg caatgaaaga tggacagtta tttacgcaag
    52801 gaacgaccca agaaatgata aaaaaagatg tattggatga attatacgaa atggacatac
    52861 ggatttgtga gattgaaggc cgcagattct gtctttattt ctaaaactaa cattaggaga
    52921 gaagagatga aaaaattagt agtaggtatc ttgattctag catcatttgg tttagcagca
    52981 tgtggaaata gcaacaacac cagtcaggca gacacaaaaa aaagcagcac acaaactgaa
    53041 actacattga cgataacaga tagtaatgga gaccaaatcg aggtgccaaa taatccagaa
    53101 aaagtcgtcg tattcgacaa tggttcttta gatacgatgg atgctcttgg tgttggagat
    53161 aaggtagttg gagcagctac aagcagcctt cctgagtatc tttcatccta taaaaaagta
    53221 gaatcagcag gtggaataaa agaaccagac ttagaaaaaa ttaatcagct tcagccagat
    53281 ctaatcatca tttctggccg acaaagagat tttcaaaaag atctaagtca aattgctccg
    53341 accattttcc tttctttaga tgcgaaaaat ccatgggaat ctttccagca aaatgtcact
    53401 gctttagggg agatctttgg taagcaagaa gaagctaaaa ctcaattaga ggaattatct
    53461 agtgcaatcg atcagacgaa aaagaaagca gaagctactg acaaaaaagc actagtcaca
    53521 cttgtgaatg aagggcaatt atcagcctac ggatctggtt ctagattcgg tttcatccat
    53581 gatttatttg gctttgaaca agcagatgat caaatcgaag catccactca cggtcaaagt
    53641 gtttcttatg aatatgtgct ggaaaaaaat ccggatatct tatttgtagt agacagaaca
    53701 aaagctattg gtggagacga cagcaaagat gatatttctg ccaatgaact ggtagcacaa
    53761 acgaatgctg gtaaaaatca gcagatcatc tcccttgaac cagatgtctg gtatttaagc
    53821 gggggcggtt tagaatcaat gaaactgatg atcgaagatg tgaatcaagc atttaaatag
    53881 cataaataac agaggattaa actctcatac tatgtaacaa taatgtttct tacattctta
    53941 aatagcaaga gcctatcctc aaagaagtta tcaaaaacca tccgtaaaat cgtattgatg
    54001 attttacgga tggtttttat ttatttgaga aatctttttt caacgtgaat tcattatcta
    54061 catcatacgg gttgattcta gaatggggag tggcaaaaaa tagtgtgatc cattttttca
    54121 gcatcaagat cgcctccttt gatttgaatc aaaggtacaa agcgaaactt agataaaact
    54181 taaatttaga tatttttatt ttgtaccgat ctcttcttta aacgatgttc catctgctgc
    54241 ttctcggcaa taagtccact agactagaaa atatgagaag ctattttctg tccagcgttc
    54301 ttattactca aagcaaatcg cagttgtcac aatctcttct aaacaatgtt caatcagctg
    54361 accttcggta ttagctcaaa gcactgattt cacaacctct atttaggcat gtgtctcagc
    54421 aagcggttga tagttgatga tctgcatttt tcgataaaaa tcggcgggaa gcttactcca
    54481 ccgttttaat aattgataga aataactctc ttctgctttg attttatact cgcttaatat
    54541 caatccaaaa ctattcgttt tttttgaagc taatttttta taaatatcca atgctttggt
    54601 tttatcttca ttgacaaaga gtgtttttac ttcaatataa gaaccctcga tattttgtgt
    54661 ttcttcgata aaatagatgt tttttatata ttccttcata gtgattcctc caaagaagtc
    54721 ttttatttga taagtttact ctaccgaaga gttgttaata atccgtgaac aaaaagtaat
    54781 tctttttcca atagttgtta aaggtttttt attttttata gtattttttg ttatttaaga
    54841 aaggaataag gttaaacaaa taacaggttg ctttttgggt ctttttccgt tcaagaaact
    54901 gaacgaagaa aagggaaagt tgtctctttt gtgataagag aaatcataat aaacgaatga
    54961 cttactttcc aataaattga gaacagtcag aaccagacaa attagttgca tctgatgaat
    55021 aaagtgatta ttctatgaca tgaggtgata cagatgaaat tagaagtgac aaaagaagca
    55081 caagaagtat tgaagaataa attagaagca gacgatcaac ttttattaga tatcgaaaac
    55141 ggtgacggtc catttgcaga aagccaagtc agttgccaat tggatacggc atttcgattg
    55201 atcattgtca aaaaagatac acaaacagat ctaagtcttt attctgtagt ggctgataca
    55261 ccagttggac caatcaaaat caaagacagt gcaatacttt acatggatga tccatccaca
    55321 cttgctttag aaccaactta taatagttta caattaaaag gaccaagcgg cttgagaaaa
    55381 ggcaacttgc aagttgttcg aaaagactag agggataaaa tgggaaaatt ggcgatcatc
    55441 agcgacctac atgtggacat caataaattc ggcgaatcag agctgatgca gctttggcaa
    55501 gttttgcaaa agcacaagat cactcggctt catctagccg gagatacggc taataaagta
    55561 gaccgttgcc tagctgttgt cgaatttttc gtcaataaag gattatctac gacatttcat
    55621 ttcggcaatc acgagttggc agatgtgccc aatgaacaga tgatggacca ttttccagac
    55681 ccacattttt taaatgaacg gtatattgct ttaaatgagc agaccgttct tttagggatg
    55741 aatggctggt acgactatca gttttccgaa ttgaaagatt acgaggcaat cgtacgaatg
    55801 aagcgtttgt attggtatga tcggttcatc cagcgtggga aaacagatct cgaggtcaat
    55861 gcagaaatgc ttgcagctgc aaaaaatttg ctagaccagc tggaagcaaa aaaacttgag
    55921 gtgattttat ccactcattt cgttcctaaa cgggcattta tcatgtatca atctgctcct
    55981 tatgagcgtt ggaacaaatt aaatgcattt ttaggatcag aatcattcgg agaattactt
    56041 gaccgttatt caaatatcaa acaagtagtt tttggccata cgcatcgtcg ctttgaagat
    56101 aaatccatcg gtcacacgat ttacagctgc agaccttttg gttattatta tgaatggcag
    56161 ctgacccgag attttgtctt gaaagaacag ttgctagaca gctatgatcc tatgaaatta
    56221 cgtgtcctct tgcgtgccca tcacccagct tttcaggcct atcaaacaca acatttgcaa
    56281 gaggaatttg aacaagcgat gacaatcgtt ccgtattgag acagcattag taatgtgtaa
    56341 aagaaaatac cttgtaaaac ataaggataa gtaagagcag tagatccatt tatcgtggat
    56401 atgctgcttt ttttgcggaa aataatcagc gaatgtttat ttgatcggat gatttgtaac
    56461 tcgcagagta ctagatgcaa gcgtgtattc tttttttgaa cgataatcac aaaaaaaaat
    56521 ata
//
LOCUS       DACEEZ010000009        55907 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20313_2017
            SAMN12639966-rid9879223.denovo.009, whole genome shotgun sequence.
ACCESSION   DACEEZ010000009 DACEEZ010000000
VERSION     DACEEZ010000009.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639966
            Sequence Read Archive: SRR10023158
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 55907)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 55907)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741683.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741683.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:44:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,016
            CDSs (total)                      :: 2,962
            Genes (coding)                    :: 2,889
            CDSs (with protein)               :: 2,889
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 36 of 73
            Pseudo Genes (incomplete)         :: 29 of 73
            Pseudo Genes (internal stop)      :: 18 of 73
            Pseudo Genes (multiple problems)  :: 8 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..55907
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639966-rid9879223.denovo.009"
                     /isolate="E20313_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            210..1424
                     /locus_tag="IWO89_000608"
     CDS             210..1424
                     /locus_tag="IWO89_000608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ammonium transporter"
                     /protein_id="HAQ0455389.1"
                     /translation="MNTLPINTGDTAFMILCTAMVCLMTPGLAFFYGGLARKNNILTI
                     MSQSFLSVGITTIMWVFGGFGLAFGRDIGGVIGNPADFFLMRHVTSFPNMFHGATIPF
                     LMFFLYQLMFIVITVPLMTGAFAGRLNLKGYIILVIFWNLLIYFPVCHWIWGGGFLDQ
                     MGFRDFAGGAVIHTTAGFGSLACILTLGQRKIPLGERSQHSSLMAAAIGTGLLWFGWF
                     GFNSGGALRADEQAVNAFGSTFIALAFAMITWLIIAKVKGNGFDFVDVLTGSVAGLAA
                     ITPCAGYVEAKSAMLIGIIAGIVCHAAVDFRKKKQWDDALDVWGVHGMGGFTGTILIG
                     IFASNSHLLTNPSSWYFLGIQVIGVIITAVYAYVLTTVILKSAMHFTTITTTKEEQEE
                     GLDFFFFGNKLN"
     gene            1455..2882
                     /locus_tag="IWO89_000609"
     CDS             1455..2882
                     /locus_tag="IWO89_000609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAQ0455390.1"
                     /translation="MQQSKTKIPAKKLTFFGFLAMTISMVVSLYEYPTFATSGFSLVF
                     FLLLGGLLWFIPVALCAAEMATVKGWEKGGVYTWVSRTLGKRFGFAAIFFQWFEITVG
                     YLTMLYFLTGALSYATGIGAIQNNKYLKLAILLIIFWAILLSQLKGTKYTSLIARIGF
                     VAGILLPALVLFGLGIHYVASGAPLQTILSMKTLIPDFSKLPTLVVFVSFILAYMGVE
                     TSASHANEMENPKKDYPLAMFVLVILAIILDTFGGLTVATTVPQHGLSLNTGVIQSLE
                     FLLRHIDPNLAWIAKILAILVCLGVIGEIASWVTSPSKALHVAAEEGLLPEYFAKENT
                     HGVPAHLMIANGIVATIWAAVFTLSGGGNNLSFLAAMSLTVVIYLMLYFMFFAGYLKL
                     VFKDDSLERAYQVPGGKVVKVIVACLGLLTSLFAFCISFVPPASIAAGQHGIYETILI
                     VSYLIVLVIPFTIYACRHKFQHAEK"
     gene            2905..3831
                     /gene="glsA"
                     /locus_tag="IWO89_000610"
     CDS             2905..3831
                     /gene="glsA"
                     /locus_tag="IWO89_000610"
                     /EC_number="3.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaminase A"
                     /protein_id="HAQ0455391.1"
                     /translation="MEVKELEQLIGKNFHYYQTGQVADYIPALAEVDPKQLGMAIYDL
                     DTKQLIEGGDADVRFAIESMSKVPVLLLAIKDNGIEKVFQTINTEPTGFAFNSIMNME
                     INHRKHPMNPFVNAGAIATTSLIAGKDADEKFERILSFMKEICDDPNITLNEEIYHSE
                     SRTGDINRSLAYYMKGNQMIEGDVPEILDAYFRQCSLNVTATGIAKLAAVLANKGIAP
                     WNGKQIITEESATIVKSIMTTAGLYDESGEFSVHVGVPAKSGVGGGLMAAVPNRYGIG
                     VFSPALDPFGNSAAGIQLLKDVVKELDADIFE"
     gene            4039..5499
                     /locus_tag="IWO89_000611"
     CDS             4039..5499
                     /locus_tag="IWO89_000611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0455392.1"
                     /translation="MLENYIERNIFRKVYLCEQLFEFQEIDIEQTAISLRVTTPTILH
                     DLESLAECLEYCIKEQVREKHKYKLVFKHGIALSELTQFLYGQSYFLKFLSYYFNGIF
                     TSTELADLEFISLSKVYTIKKIVLDFFKENDYLKNKEIIIPEFDSRNILLALVRYVNW
                     EGYENKNPYVEEACNELIDYVETHFFKRRYSEDEKSFIIRGIEIAIGRKAFPLGLKEE
                     DKKSAETKPLFQLVCQGLEEQKGILDLQEEDVYYIFSLFNSRNYTNENMELLRKDFEV
                     VYKNFVLNDLSLNELILQIISIAEIDDAENFIFKQSFLQFVRTLWADGQVFLPERIYL
                     LSEKEQLLYGKIVEILEEWKKKNQIQLRWNENFIRKFVKSLSLINSEQAGRQEIEIFV
                     VSESSVKQFFYRTQFHLYAEEGIAEINPILFRSLEELPDECLCAKKRVVLCDAASYQN
                     GLETEKTKIYPISLKGANIHLYHIAQELHLLKKHIS"
     gene            5720..7483
                     /locus_tag="IWO89_000612"
     CDS             5720..7483
                     /locus_tag="IWO89_000612"
                     /EC_number="2.2.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate synthase"
                     /protein_id="HAQ0455393.1"
                     /translation="MSTVLHVLDAINSPADVKKLSVEEMKQLAEEMRQKILQKDSDVG
                     GHVGPNLGVVELTIAFHYVFDSPKDKIVWDVSHQSYGHKLLTGRKNGFEKGHYREVSG
                     YSNQHESKHDYFTVGHTSTSISLATGLAKARDLKKESGNVVAFIGDGSLSGGLAFEGL
                     NNGGVLQSNLIVLVNDNEMSIDENHGSLYKNLAELRQTNGKSSNNLFKAMGFDYRYVD
                     NGNDLETVIRLFEEVKDIDHPIVLHVHTEKGRGYQPAIDNKMKYHWHVPFDLSTGEAK
                     QVTSGETFNMIIMDYLDKKMQTSTAPIAAVNAAIPGAFGLKTFQEKHPDHYFDVGIAE
                     QHSITFASALASQGVRPVVFQSSTFLQRAYDQLSHDLAINENPVIILVGGGTISASDR
                     THQGTFDIPYLSSIPNIKYLAPATKEDMISMLDWAFEQTAQPVAIRIPTHGVEHGTAI
                     ANDYSIPAYQTVNNGGKVAILALGGFLELGKETVKAFRKKGIEATLINPGTITENDVE
                     TLDKLKETHELVITLEDGSLSGGFGENISRYYGDSSMNVLNFGAEKEFTDSVPVGQLY
                     ERYHLTPKQIVEDSLRVLNIQ"
     gene            7567..8025
                     /locus_tag="IWO89_000613"
     CDS             7567..8025
                     /locus_tag="IWO89_000613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ASCH domain-containing protein"
                     /protein_id="HAQ0455394.1"
                     /translation="MYQQVEEYVKKFQEKYPSYQSEKLGFFSFGDTEKMADELSELVV
                     KKIKTGTSSGYDLYKQENEPLPKVGQLDVVLNGKNEPVCIIKNINVTILPFSEVTKEH
                     AWKEGEGDRTLAYWRKVHAEFFKHAYKEALNIEFTEEKLVVYEEFEVIFF"
     gene            8134..8817
                     /locus_tag="IWO89_000614"
     CDS             8134..8817
                     /locus_tag="IWO89_000614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine-6-phosphate 2-epimerase"
                     /protein_id="HAQ0455395.1"
                     /translation="MDTLEQIKGGVVVSCQALENEPLHSSFIMGRMALAAKEGGAVGI
                     RANTTEDINEIKRIVDLPVIGIIKRDYDGSEVFITATEKEVEELLETQAEIIALDATS
                     RKRPTEKTTAELVQMIHEHGRLAMADISTFEEALQAQEDGFDLLSTTLAGYTEYSRKT
                     QTPDFDLLNQIIEQVDIPVIMEGHTDSPEQVEKAYELGAFSVVVGSIITRPQLITKRY
                     VEAASKANN"
     gene            8964..9578
                     /locus_tag="IWO89_000615"
     CDS             8964..9578
                     /locus_tag="IWO89_000615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase family protein"
                     /protein_id="HAQ0455396.1"
                     /translation="MFERSPSELTERENYKLLIGSVIPRPVAVVTTKSAKDIVNIAPF
                     SYFNIVSSNRPILSLAIQRKQDAVKDTAKNILETREAVIHILDEDNVEEVNKTAANLP
                     NEESELYLTDLTLTDSVIISVPALTEAKVRFETSLYEHVEIRNKNKVTADLFLLEVQK
                     YHLNETVYDDKTGRIDPRKLNAVSRLAGNDYAAIGEIFTIARPE"
     gene            9594..9800
                     /locus_tag="IWO89_000616"
     CDS             9594..9800
                     /locus_tag="IWO89_000616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0455397.1"
                     /translation="MLKISYKPSTDSKEMKKEYETVNDFLQGQYLEVPPLQDHFVVTT
                     VTLDGKEIEMPDQTISGLFNYFNK"
     gene            10339..10896
                     /locus_tag="IWO89_000617"
                     /pseudo
     CDS             10339..10896
                     /locus_tag="IWO89_000617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303250.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
     gene            10954..12993
                     /locus_tag="IWO89_000618"
     CDS             10954..12993
                     /locus_tag="IWO89_000618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HAQ0455398.1"
                     /translation="MKRSDKHGKNRTGAYIAGAVILIAAAGGGYFYYQHYQETQAVEA
                     GEKTVEQFVQALNKGDYNKAAEMTSKKAANKSALSEKEILDKYQNIYGAADVKGLQIS
                     NLKVDKKDDSTYSFSYKAKMNTSLGELKDLSYKGTLDRNDGQTTINWQPNLVFPEMEG
                     NDKVSLTTQEAARGNIIDRNGEPLATTGKLKQLGVVPSKLGDGGEKTANIKAIASSFD
                     LTEDAINQAISQSWVQPDYFVPLKIIDGATPELPAGATIQEVDGRYYPLGEAAAQLIG
                     YVGDITAEDIDKNPELSSNGKIGRSGLEMAFDKDLRGTTGGKLSITDADGVEKKVLIE
                     HEVQNGKDIKLTIDAKAQKIAFDSLGGKAASTVATTPKTGDLLALASSPSYDPNKMTN
                     GISQEDYKAYEENPEHPFISRFATGYAPGSTFKMITAAIGLDNGTIDPNEVLTINGLK
                     WQKDSSWGSFKTTRVSSDVSQVDLKTALIYSDNIYAAQETLKMGEKKFRTGLDKFIFG
                     EDLDLPISMNPAQISNEDSFNSDILLADTGYGQGELLITPIQQAAMYSVFTNNGTLVY
                     PKLIADKETKDKKNVISETAVQTIVPDLREVVQDVNGTAHSLSALGIPLAAKTGTAEI
                     KEKQDVKGQENSFLFAFNLDNQGYMMVSMLENKEDDDSATKRASELLQYLNQNYQ"
     gene            complement(13102..14253)
                     /locus_tag="IWO89_000619"
     CDS             complement(13102..14253)
                     /locus_tag="IWO89_000619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:proton antiporter"
                     /protein_id="HAQ0455399.1"
                     /translation="MEFIGILCLILISTTIGSHISRRLGIPAVIGQLLVGVLLGQAGL
                     NWVKPDILVHDFSEIGVILLMFLAGLESDLSLLKKYFRPGMFVALLGIIFPVVFGWLT
                     GKAFQVSGNEAIFFGIILAATSVSISVEVLKELNVVNTKEGSTILGASVVDDILVVLV
                     LSFSLSFLTGEASSNASLPLVLIEQLIYFVFIFLLVKWIAPFLLSFAEKVYANSSIII
                     VSLVICLGMSYVADLVGLSSVIGAFFAGIAVSQTKVKEEVYHNVEALGYAVFIPVFFV
                     SVGLEVDFSDFSEQLLFISILTLVAILTKLLGGYFGAQIAGFSQHSSLMVGAGMISRG
                     EMALIILQIGQQSQLIEPQYYSPLVIVILLSTLISPLVLKYFTKKVYEV"
     gene            complement(14407..14937)
                     /locus_tag="IWO89_000620"
     CDS             complement(14407..14937)
                     /locus_tag="IWO89_000620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0455400.1"
                     /translation="MFETIKQNIQKYALLRAAVYIISGIAIVLNPSAVFHFIGYLITA
                     YLVLLGLLNLFEAYRFKRKTGSWSIGLFSGLFFLILALIVYAFAPAIVSILPILLGLA
                     VIINSLFQLFFSMNTKAKSWSVYSSILLLIGGFVLLFNPFKSLMLLFQIFGFILIFMG
                     IQEIINFIRIRKMTIR"
     gene            complement(15036..15386)
                     /locus_tag="IWO89_000621"
     CDS             complement(15036..15386)
                     /locus_tag="IWO89_000621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2200 domain-containing protein"
                     /protein_id="HAQ0455401.1"
                     /translation="MAQNRIFTTSFASVYPMYVAKAERKDRIKEEVDEIICWLTGYSQ
                     TELEYQLQKQVTFEQFFAEAPVPNPSRSLITGVVCGVRVEDIEDNLMKEIRYLDKMID
                     ELAKGKKMEKILRS"
     gene            complement(15543..17072)
                     /gene="ahpF"
                     /locus_tag="IWO89_000622"
     CDS             complement(15543..17072)
                     /gene="ahpF"
                     /locus_tag="IWO89_000622"
                     /EC_number="1.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkyl hydroperoxide reductase subunit F"
                     /protein_id="HAQ0455402.1"
                     /translation="MLDKETKQNLEQYLALIESPIVFSVSLDNSENSQKLAEFTKEIA
                     EMSPKISWEKRDSMRTPSFSINPVGKESGIVFAGIPLGHEFSSFLLAMLQISGRAPKI
                     SESLSLKIKKITQTLHFDSYVSLTCHNCPDVVQALNILAVLNPNITHTMIEGGMFQKE
                     IDDKKIMAVPTVFLNGEEFSSGRMTIEQIVEKITGPLIEEELFEKEPYDVLVIGGGPA
                     ASSAAIYAARKGIRTGLVADSFGGQVVETLGIENMIGTSYTEGPKLMQQVESHVRSYP
                     IDIMMNQQAVSLNKEQHFINIGLANGISLKAKTAIIATGAHWRSINVPGEKEFKNKGI
                     AYCPHCDGPLFKGKEIAVIGGGNSGVEAAIDLAGLAKHVYVLEFLPELKADQILQDKL
                     VSLANVTVIKHAATKEISGTNQVESLSYIDRRTMEMHTLAVSGVFILVGLLPNTDWLD
                     RTIEMNPRNEIVTEKNGATNIPGVFAAGDCTDSPYKQIIVSMGSGATAALGAFDYLMR
                     T"
     gene            complement(17082..17645)
                     /gene="ahpC"
                     /locus_tag="IWO89_000623"
     CDS             complement(17082..17645)
                     /gene="ahpC"
                     /locus_tag="IWO89_000623"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003756453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HAQ0455403.1"
                     /translation="MSLIGKKIEEFNAHAYHQGEFLEVSEKDLMGNWSILCFYPADFT
                     FVCPTELEDLQEQYSTLKSLGVEVFSCSTDTHFTHKAWHDTSDAIGKIEYVMIGDPSH
                     QISRIFDVLDEEQGLAQRGTFIIDPDGIVQAMEINADGIGRDASALIDKIRAAQYVRT
                     HPGEVCPAKWKESGETLKPSFDLVGKI"
     gene            complement(17789..18451)
                     /locus_tag="IWO89_000624"
     CDS             complement(17789..18451)
                     /locus_tag="IWO89_000624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAQ0455404.1"
                     /translation="MKSSLQSQAYQAIRKQIIYADLEPGRKISEKALEEHLAIGRTPI
                     REALIQLRQQELVYTIPQSGTYVSKINLVSAENARFVREQLEEQIMIECCSKLDTQTR
                     IVLETILEEQEKAVKINDERAFFRADNLFHEACFEIAGRKEVWNWLDDHNTHLERFRW
                     LRVTIQGLPWETIMEQHYDLLKALVDKKPEEARFQTGLHLHMMLKEQETVTQRYPEYF
                     QQ"
     gene            18635..20281
                     /locus_tag="IWO89_000625"
     CDS             18635..20281
                     /locus_tag="IWO89_000625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol dehydrogenase family protein"
                     /protein_id="HAQ0455405.1"
                     /translation="MIDLNNWRNFIPELSQKEVEIPHFDRASLRENGQKNPVWIHFGG
                     GNLYRGFHAEIAQRLADLGELSTGVIVCETFDEEIIERAYQDFDDLILEVVMPSSGEF
                     VKRILAATADSLYCHPDCSEHFDKMIRHFENPSLQLVTFAITEKGYRLKDTNNKYIGP
                     IAVDIKNGPKKPSHTMSILAVLLYKRFLSGAWPIALVSTDNFSQNGSQLRTSLLTIAK
                     EWQKEGYVQNTFIQYLSNEEIVAFPWTMIDRITPNPSLSVSKMLAKEGFSNMSIFQTA
                     KGNNIAPFVNTEDIHYLIMEDHFPNGRPKFEKAGVFLTDRATVDKADTMKVTTCLNPL
                     HTSLAIMGCLLGYRSIAEEMKDPDLSLLVSEIGYNEGLPVVESPGIIDPKIFLEEVIT
                     VRFSNPNIPNMPQRIATDTSQKVAIRYGETIKKYMEIEVKDPQTLVFIPLTLAAWLRY
                     LIGIDDQGKPFIPSPDPLLTDLQHKLSDIQLGTQDTSHIHEKMAPILSNKEIFGVDLY
                     QAELGRKIEEMFIKMVAGPNAVRKTLQDYLQEHGGNLSWK"
     gene            20272..21354
                     /gene="uxuA"
                     /locus_tag="IWO89_000626"
     CDS             20272..21354
                     /gene="uxuA"
                     /locus_tag="IWO89_000626"
                     /EC_number="4.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannonate dehydratase"
                     /protein_id="HAQ0455406.1"
                     /translation="MEMTFRWYGSQEEKITLDEIRQIPAVKGIVGTLMDIPVGEVWPR
                     ERIKELKEEIESRGLTLKVIESVNVHDAIKIGLPSREQYIENYKQTIRNLAEYGIEVI
                     CYNFMPVFDWVKSDLDYRLEDGSSTLAFISDDIPDDPREIVARIEHSSNEFGLPGWEP
                     ERLAPIKSLLEAYASVDEEKLRENFAYFLQSIIPTCEEVGVKMAVHPDDPPYSIFGLA
                     RIVKNREDLDWLCRIVDSPYNGITLCTGSIAEDPENDVYGILAEFCQRDRIPFAHVRN
                     IKFLANRDFYEAPHKSEYGSLDMYRILKTMYDNGFNGYIRPDHGRMIWNETGRPGYGL
                     YDRALGAVYLNGLWEAIEKGNKSKDN"
     gene            complement(21462..23015)
                     /locus_tag="IWO89_000627"
                     /pseudo
     CDS             complement(21462..23015)
                     /locus_tag="IWO89_000627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738699.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="M protein trans-acting positive regulator"
     gene            23163..24304
                     /locus_tag="IWO89_000628"
                     /pseudo
     CDS             23163..24304
                     /locus_tag="IWO89_000628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318369.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
     gene            complement(24430..25509)
                     /locus_tag="IWO89_000629"
     CDS             complement(24430..25509)
                     /locus_tag="IWO89_000629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HAQ0455407.1"
                     /translation="MNNVFIFCYLEVVDLTVYQRFLANEKIRRLTVLLFVVFVLFLVR
                     SMITTILLTFIFTYLMIHLVQIIQRFVRIPTGVLAIMIYTLIVYLIYLAFTKYIPMLI
                     HQTFDMIQSVIDFYEHQPKDADPVLQYIHTYIERNDFFEQLQNGASIALGYLQDIGKM
                     AVAFAMSFILSFFFMIEKKKTIVFSRLFLKSEFSWFFQDIYYFADKFVNTFGLVLEAQ
                     FVVALLNTLLTTIALAVFGFHQLLSLAIMIFILSLIPVAGVIISCIPLSFIAYSQGGI
                     RDVVYILITILIVHLIESYVLNPKLMSSKTELPIFYTFVVLLVSERFFGVWGLIVGIP
                     IFTFFLDVLKVKKIPNHLPKETDIP"
     gene            complement(25618..26058)
                     /locus_tag="IWO89_000630"
     CDS             complement(25618..26058)
                     /locus_tag="IWO89_000630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HAQ0455408.1"
                     /translation="MEDLYLVNQVCFPLYAASKEIIRHYTSLLKPLNLTYTQYLVMLV
                     LWEKKKTSVKELGNDLCLDSGTLTPLLKKLAEKNYINRQRETGDERIVNVTLTPQGAA
                     LQKQAEGIPQKIKSYLPLEQEELDTLQHLTRKMLDNFHQDQKKG"
     gene            complement(26115..26525)
                     /locus_tag="IWO89_000631"
     CDS             complement(26115..26525)
                     /locus_tag="IWO89_000631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="organic hydroperoxide resistance protein"
                     /protein_id="HAQ0455409.1"
                     /translation="MKKMYSTKMINTGGRSGEVHNPDRTFELNIAAPGKRVENATNPE
                     QLFAAGFSACFNSALELIKQQKNISGASTVSAQVSLYSESETSFVLGVEIEGSIEGLS
                     LEETQELLEAAHKVCPYSKATAGNIEVTLKAVSE"
     gene            complement(26706..27140)
                     /locus_tag="IWO89_000632"
     CDS             complement(26706..27140)
                     /locus_tag="IWO89_000632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HAQ0455410.1"
                     /translation="MHKGTEIDFVLNDSLKALELYQTIFDEIQLIEATDYPRGNNEVI
                     FTLYGTRFHMLDENTSLGLTAPTAEHPNTIWFNIIVSDIEDTHNKALRNGCVEIQEMT
                     EMMDFGVTNSMFRDPFGYLWMLHQVHREVSFEERKQLFEKYM"
     gene            complement(27240..28088)
                     /locus_tag="IWO89_000633"
     CDS             complement(27240..28088)
                     /locus_tag="IWO89_000633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HAQ0455411.1"
                     /translation="MKETRQFIWTYIKIIFALFILGVAINMFLGPHHIAAGGVSGLGI
                     LLEAVLGFDRAIVILVLNIVMLVLALLFLGKKPFFKVLFGSLFFPLIIAIVPETMITS
                     DRLLSVIFGSAIFALGVAILYKNNSSSGGTTIPPLIFKKYFHLNTSIGLFFTDAFVVS
                     LNLFVFGIEEFLYAILSIAITSIVMTYIETGINRKKSVMVMSEHYLEDIRLRLSAEIG
                     RGLTLLEAKGGYNRKSKEVLLIVVTDHEFSRMKPLIEEIDPSAFVIVSSVAEVMGRGF
                     TYHPIE"
     gene            28477..30243
                     /locus_tag="IWO89_000634"
     CDS             28477..30243
                     /locus_tag="IWO89_000634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008271692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glycosidase"
                     /protein_id="HAQ0455412.1"
                     /translation="MDTAAIYHRPESEYAFLYTNTRFRIRLRTRKNDIKKVYVLCGDP
                     YTITTEKWYQKQRPMKKCLSTNVHDYWEIEVTSETRRLQYAFHVVGEDNTDCFYGDQG
                     IFPYRENVITEPNFYFRIPYFHQIDRFTIPEWVKETVWYQIFPERFANGDKANDPEHT
                     LPWGSKDPDREDFFGGDLQGVRDHLDYLTDLGVNGIYFCPIFKATSNHKYDTIDYYEV
                     DPAFGDKKLLKNLIDEAHKRGIRIMFDAVFNHMGVHSPQWQDVLKNGEKSIYKDWFHI
                     RFFPVDSYQMTDLPETAENIPYDTFAFTPFMPKLNTANPEVQKYLLDIATYWIKEFDI
                     DGWRLDVANEVDHHFWKKFREAVTEIKPDIYILGEIWHSSQAWLQGDEFHAVMNYAFT
                     DSIKDYFAKKKITASQMVSGMNHQQMLYRDQVNEGTFNLLDSHDTARILTLCQGNKEL
                     MKSVMAFMFLQKGSPCIYYGTEIGMTGEDDPDCRKCMIWEKEEQDLELFGFIKELVSL
                     RKQLSKIISEGSTQWLIVYDREDKLYFTRELEGQIIYVYFNQSKEPWVVEQENEVILS
                     QNCQLLEDGKAEIRQYGFLIMC"
     gene            30345..31604
                     /locus_tag="IWO89_000635"
     CDS             30345..31604
                     /locus_tag="IWO89_000635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HAQ0455413.1"
                     /translation="MKKRSVKQLGLGLISLGILGGLAACGNGNSSAEGEADEGKTLTV
                     SVDSGYKDYINEIKNTFEKENDVKIKLVEKDMFDQLESLALDGPAGKGPDVMMAAYDR
                     IGALGQQGHLAEVKLGNEAAYDETDKAQVTYDGKIYGEPAVIETLVLYYNKDLVDTAP
                     STFKDLENLSKDSRFAFESEAGKNTGFLAKWTDFYYSYGLIAGYGGYVFGDDGTNPSD
                     IGLNNAGAVEGISYATDWFKNVWPKGMQDIKSAGDFASQQFMSNKTAAIIDGPWQAQT
                     YKENNINYGVAKIPTLNNGQPYQPFGGGKGWVVSNYAKNKDLSQKWLDYVTNQENQEK
                     FFEMVNEIPANQQARETAKAKNDELTTAVIEQYETAQAMPNIPEMGEVWTGAENLMFD
                     AASGSKTPKESADEAVKTISEAIEQKY"
     gene            31751..33052
                     /locus_tag="IWO89_000636"
     CDS             31751..33052
                     /locus_tag="IWO89_000636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAQ0455414.1"
                     /translation="MQQDKTSNINEKKITLCSLIPGMGQFVNGQAFKGIVFFAIFVLF
                     IGQMLLGGFHALVGLVTLGSIPMEDHSLFILIEGTLQFIITIIFLIFYGLNLLDAYRV
                     AKMRQEGKKINRTMKEVIHNIGDEGFPYLLTLPAYLLMIFTIIFPVLVTLFTAFTNYD
                     FNHIPPASLIDWVGAKNFFSIFFLSSYRNTFFAVFSWTVIWTLCATTLQITLGILTAV
                     VAHQKFIKFKRIFGIIFLLPWAVPAFITIMSFSNIFNDSVGAINTQVIPFINQLPFVD
                     ISAISWKTDPNWTKVAIILVQGWLGFPYIYVMVTGILQSISEDLYEAAKIDGANAWQR
                     FSNITLPAVFLVAAPTFITQYTGNFNNFSMIYLFNNGGPGSVGGGAGSTDILISWIYK
                     LTTGTSPQYSMASAITLIISVIVICVSLLVFKKTNAFNMGD"
     gene            33055..33906
                     /locus_tag="IWO89_000637"
     CDS             33055..33906
                     /locus_tag="IWO89_000637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAQ0455415.1"
                     /translation="MKKFHSSARFQKRLSHFFTYFFLLILSVIIIYPLLITASTAFKS
                     GNIAAFSLDLSSDWTLGNFTRLFSETLYGTWYKNTLIISVLTMIVQVTVVTLAGYTYS
                     RYRFIGRKSSLMFFLVIQMVPTMAALTAFYVMAFLLEALDKYWFLTMIYIGGGIPMNT
                     WLMKGYFDTVPIDLDESAKLDGAGHFRIFFQIVLPLVKPMLAVQALWAFMGPFGDFML
                     AKFLLRTPENQTVAVGLQTFISDVTNQRVSLFAAGAILIALPICLLFFFLQKNFVTGL
                     TSGGTKG"
     gene            33919..34734
                     /locus_tag="IWO89_000638"
     CDS             33919..34734
                     /locus_tag="IWO89_000638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltodextrose utilization protein MalA"
                     /protein_id="HAQ0455416.1"
                     /translation="MQTFPLNYFSALRTPTQVFAGRKLLSWPKFFLIFVFLVSLMVMP
                     VTLFYANQIQAIPLEQFLSVHSLIDEQGTQKFSELELSETGLQASQQTIAVTPEILVG
                     VSLSEKQQSDHGTFIDFEKEQWVIQQKDKSGIRRYTMNYSPSFQPDSVRTPEDFQRFL
                     EREFYASNRPTIILSYSLSLGLVLFVMTSLILFGASFFLWMTRKSQLSSIHTFKESAN
                     LMLNVMGIGSMAAAVVGFIHFDFILMLSVQTTITVLLLLWVFVKTRFKDKRVE"
     gene            34885..36051
                     /locus_tag="IWO89_000639"
     CDS             34885..36051
                     /locus_tag="IWO89_000639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002369828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HAQ0455417.1"
                     /translation="MLEKTLYNQLFSRSFSLPVEVNYWDGTTKQYGDTDTPPQIKINI
                     HEEIPMKEITSNASLALGEAYMDHRIEIEGSIQALINDAYQQANSFLRGKDYLKWLPK
                     KEKHTKQKNKEDIHAHYDLGNDFYKLWLDPTLTYSCAYFNTPEDTLEQAQINKVHHIL
                     DKLFIKEGETLLDIGCGWGTLMFTATKEYNVKATGITLSEEQYDLITKRIKEEHLEDK
                     CRVLLMDYRELKGETFDHITSVGMFEHVGSENLEGYFKVVKDLLKPKGTALIHGISRQ
                     QGGATNAWINKYIFPGGYVPGVTELVDNITKNDLQLIDLESLRRDYQLTLEHWTKNFH
                     QVQDQVTKEMGDSFYRMWDLYLQACAASFESSNIDVIQYLLVQPGNNDIPMHRA"
     gene            36281..36928
                     /locus_tag="IWO89_000640"
     CDS             36281..36928
                     /locus_tag="IWO89_000640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysis protein"
                     /protein_id="HAQ0455418.1"
                     /translation="MKKTVIYTSLFAALFLAACQSNTNQGTSGNTSSSSEQRSSQVSD
                     SSTKASTSSSSQTKSSSEASTQSDIKVSAARAIELFQDKYPDTAITSLELDSDWGSYF
                     YKIEGVDDQKEYEARIDAQNEKMETENPETLDRDEQNGQKKKEDGLDVDKLITIEEAA
                     KIAVDKVGSGTATDWDLDKDLGTTYWEVKVKDGNQSTSVKINSQTGEVLSTEQDD"
     gene            37012..37956
                     /locus_tag="IWO89_000641"
     CDS             37012..37956
                     /locus_tag="IWO89_000641"
                     /EC_number="1.3.98.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate oxidase"
                     /protein_id="HAQ0455419.1"
                     /translation="MSLETTFANHIFANPLMNASGVHCMTTQELDELAHSEAGAFITK
                     SCTINERKGNPEPRYFDVPLGSINSMGLPNLGFSYYLEYALAYEKVQENQNQPLFFSI
                     AGMSVQENLEMLEKIEKSGFNGITELNLSCPNVPGKPQLAYDFEATYETLKEVFSIFS
                     KPLGIKLPPYFDFAHFDQMADILNQFPLTYVNAINSVGNGLYIDTEQEAVVIKPKEGF
                     GGIGGEYIKPTALANVRAFYTRLKPEIQIIGTGGIRTGQDAFEHLLCGASMLQIGTEL
                     HKEGPEIFSRIIKELTQIMSEKGYTSIDEFKGKLRTIS"
     gene            38091..39542
                     /locus_tag="IWO89_000642"
     CDS             38091..39542
                     /locus_tag="IWO89_000642"
                     /EC_number="3.5.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidase"
                     /protein_id="HAQ0455420.1"
                     /translation="MNDATELAKELKEKEFSFQELVTDIQKKIKKQNHELNAFVALEP
                     FESNSHELLIPQSEKGPLAGIPFPLKMLGQEKKGWLATSGSRLFETHRASHTSNYVRQ
                     AEAIGLVPFGQTNAPEFGFKNITDPVIYGPARNPWNLECTPGGSSGGAAAAVSSGIVP
                     LAGASDGGGSIRIPASFCGLIGLKPSRGTMPVGPYAWRGWQGAAIDFGLTVSMRDTEA
                     LFYGMRSIHSGAPYQVSPVEWQTHPRKKRLKIAFCVDSPIHSQISEEAVLAVKKAVDF
                     LGKEGHDLTEIPYPLDGRKLITSYYQMNGAETAAMIRSIETGIQRAVRKEELEPISWT
                     LFQYGQKLSAATYIDSLHVWDEAAIVMENLHQEYDLFLSPTTAFTAPKISEDLQSDAI
                     RAQMEHISDCSEVEGLDVVSSMFEESLKITPYTQLANLTGQPAISLPTHLTKENMPLG
                     IQFMAARGREDLLFQIGYLFEANHQFHLPNPWK"
     gene            39563..40399
                     /locus_tag="IWO89_000643"
     CDS             39563..40399
                     /locus_tag="IWO89_000643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HAQ0455421.1"
                     /translation="MKKKRLFALITSAFFILSACGTAAANQEDNSWSNVEKKKELVVA
                     TSGTLFPASYYNDQNELVGYDVDLAKEVAKRLNVKISFKEYNVDGQITSVSKRESDFA
                     ANDFGITKEREKKFSLSETFKYSFDSMIVRKKDDSGIHSLEDLKGKKAAGEPNTSYMR
                     LAEKHGAKLVTYDNATNDQYLSDVANGRTDVILNDYYLQKMAVSALPDIPVKILENVY
                     FNENESGFLLKEGNDALKKKIDGVLKEMKEDGTTKKISEKYFQTDVSVKPKETMAESV
                     SE"
     gene            40399..41058
                     /locus_tag="IWO89_000644"
     CDS             40399..41058
                     /locus_tag="IWO89_000644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HAQ0455422.1"
                     /translation="MYFPLIDFSLMWTSLPFVLQGLVYTLGIGFVSFLLGNLVGLLLT
                     VLGLLDWLPLNVFIRFYLSFFRGIPALVLLFLLYFGLPYQLSALTASVICFTITSSAF
                     IGEIYRGSLAGVSSGQWDAGLASGFSFFGVLKYIVLPQAFRISVPALSNVAMDLVKGT
                     SLAATITVPEIFQKAKIIGGRTFDYFSLYILVALIYWVICIGIEKVQQYLEKRSIHQS
                     I"
     gene            41184..41873
                     /locus_tag="IWO89_000645"
     CDS             41184..41873
                     /locus_tag="IWO89_000645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0455423.1"
                     /translation="MKTPLTIEIESSLYQYCIEQGGLVVEEVTMPDDQGIVDTLSCLT
                     KFDGSREWRCYELKVTKADFRSTAKLSFVGHYNYFVLPSKLYEEVKTEIPTGIGVLVY
                     RPYTVEEEMPASGTFLIAKKAAKKELAVPEEALTNRFMASLFREVRKAKQMERGVKYF
                     PKDQLYKELKRRSENQDPFSNERYYERFLEEIASQAINELQEEVIALQHDYEYLRQQQ
                     QIRRLPTEPLE"
     gene            41879..42835
                     /locus_tag="IWO89_000646"
     CDS             41879..42835
                     /locus_tag="IWO89_000646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sce7725 family protein"
                     /protein_id="HAQ0455424.1"
                     /translation="MYYPYLRGKQFDLLALKEALSRGLLSNKIQPVIEPVRDSATLKN
                     VIELFQKKHHPIAVIQNPQVGQFKLFDQHVHSWTVKENSSVVPAQILTPENLSEVLDS
                     PPAFLVFDGQHYPKDTEVWKQLAGVDSKFLIPDTSRFRIWLPENKIVIRDSFQTRKHV
                     ESYADKTDDFFSDDYLFFHADGYIGFSDFTIEGSRYFDKGFPSRALAIHLTYIDAYGN
                     IRVKHFVSDSNDSAKDQALKFLEAGDKMKKWIMRHHHQLLITSGMIELLALYQQQKFP
                     GLGVLKKWSLMHHLELISHLLDHPKDWLKYYSKVQELYEVIQ"
     gene            42879..43391
                     /locus_tag="IWO89_000647"
     CDS             42879..43391
                     /locus_tag="IWO89_000647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAQ0455425.1"
                     /translation="MYIRQTRKEEVEEVVEIINAAADFIASQGSPQWQNGQKPTKETI
                     LNDIERKESYVLIVEDQIAGTAALVSGVDPVYTKIDGSWKESTGPYLSIHRVALAEST
                     RGKKLAKPFLEHLLTIAASKNIQDIRIDTYPANEPMEKTIYSAGFTYQGMIEFPFEHG
                     ERKAYQIIIN"
     gene            complement(43647..44195)
                     /locus_tag="IWO89_000648"
     CDS             complement(43647..44195)
                     /locus_tag="IWO89_000648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HAQ0455426.1"
                     /translation="MNYCYVFIGPSGSGKTSLAEAIFQPEQKIITYTNRSPRPGEKDQ
                     EDYYFVSDNTFNQMIKGNEFAEWDQYAQHKYGSSKAEIKKKLTVGNCYAVLTANGFWH
                     LYEYFGTVVRPIFITISKETLQSRLKKRGDSSEQISARLAVYVEDLAQLKRLKAIPSL
                     LVVSNDGAFQEAADKIKKGLLI"
     gene            complement(44284..45465)
                     /locus_tag="IWO89_000649"
     CDS             complement(44284..45465)
                     /locus_tag="IWO89_000649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NupC/NupG family nucleoside CNT transporter"
                     /protein_id="HAQ0455427.1"
                     /translation="MSYLLGIIGLFVTLGLAFLISSNKKAIKLKPLIVMFVLQLVLGF
                     VLLRTTFGTAVVSVLAKMFDHLLAFAGQGVDFVFAGVANKGSAPFFLSVLMPIVFISA
                     IIGILRYIKILPLFMKAVGLGLSKINGMGKLESYNGVASAILGQSEVFISIKKELPFL
                     SEKRLFTMSVSAMSTVSMSIVGSYMALIDSKYVITALVLNLFGGYILASIVNPYVLEE
                     KEDELIIEENKEQTFFQMLGEYILDGFHVAITVAAMLIGFVALIAMINAIFHGIFGIT
                     FQEILGYIFAPLAFISGIPWKEAVDAGSIMATKLVTNEFVAMTELANGNFHFTERTTA
                     IISVFLVSFANFSSIGIISGAMKGLNEEKGNLVAKHGLKILLTATLVSFLSAIVTGLL
                     V"
     gene            complement(45701..47209)
                     /locus_tag="IWO89_000650"
     CDS             complement(45701..47209)
                     /locus_tag="IWO89_000650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1846 domain-containing protein"
                     /protein_id="HAQ0455428.1"
                     /translation="MKKIGFDSEKYIEEQSAYILERVNHYDKLYLEFGGKLVDDKHAK
                     RVLPGFEEDAKIKLLQKLKDQAEILICVYAGDIERNKIRGDYGITYDMDILRLIDELR
                     GYGLLINSVVITRYSGQPATKVFINKLERRNIKVYKHSEIEDYPVNVEKIVSEDGFGK
                     NEYIETTKPIVVVTAPGPGSGKLATCLNQLYHESQKGHAAGYSKFETFPVWNVPLKHP
                     LNIAYEAATVDLKDVNMIDSFHFDAYNKVAVNYNRDVETFPVIKRIIEKITGKESVYQ
                     SPTDMGVNRVGFGIVDDTVVQEASKQEIIRRYFQTECDFKKGLIDEEAVNRIKLIMEE
                     VGLRPEDRNVVVPAHDYADKIQAQSPTKDPSAVIAIELPDEVIITGRTSSLMDASAAV
                     ILNAVKHLAHIADDIPLLSPLVLETIQGLKSKTLHSSLDALSINEVLIALSISAVTNP
                     IAQVAYDKLAELEGAQAHSTVMVNKNDEQTLKKLGIDITCAPVYPSENLYYQ"
     gene            47368..47559
                     /locus_tag="IWO89_000651"
     CDS             47368..47559
                     /locus_tag="IWO89_000651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0455429.1"
                     /translation="MKKRQKKKNAYKHYIRSIFTGYERMLEDPELEQLTFTYLNEETQ
                     LTRDEHQRIHFTTRDLPSK"
     gene            47762..48679
                     /locus_tag="IWO89_000652"
     CDS             47762..48679
                     /locus_tag="IWO89_000652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="viral A-type inclusion protein"
                     /protein_id="HAQ0455430.1"
                     /translation="MKEPDTNANYEPDELEETNADQDTGQESSSFYDIKEKASMNLSQ
                     LLEDLQAFEKAVREEDIPEIYRLYNGRLNEELKRSSNENHEIDQLLMRKIHDRFLQEF
                     PFMEQVENISPTMSYYKIGTYYHDRPTVGIDASLPEIFVLPKIDEEWEKYSQPESTDY
                     DKKINELDAKVITAQTEIERLDEQIKEINRQMTDLNDNKGFLNRKKIEEEIQELEKKK
                     QVLSNEKLGWLPYIETPETIQQQKEALKQEARADQLRAAIVEKEQRQIRRYFGSKEGF
                     GQAIHEFLMNYLGNENPNNQSNEGGSEYE"
     gene            48672..49745
                     /locus_tag="IWO89_000653"
     CDS             48672..49745
                     /locus_tag="IWO89_000653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0455431.1"
                     /translation="MNEHDEAEWTKNEEQTEDHEETNTSLEEQTSNPLDDTEEIPMLE
                     ETKIHEKEKVTPTNTNTAENTHAYEITENPGYLEGKEQDHQDIVSLIETAEAELADLR
                     MRKDLIESDFPQLLESYDRFILNEEPISTIGKTSLIEYFTYELLKPLNDIGLERSNSA
                     YDTWKTRHFSINYTLNGQNELVFSFVLPTIDQRYQVESMSLLNVSPETMEINVQDDRV
                     LSLIRHWSVDRVFSAGQITIFNHKLNQLLAHARTLGFTVNQTLLDNTKPLRLNLQSEF
                     ELTNEVLDDIFIVAMKNPSYDMEKINETTYQVRLDKGQSLTITTNEKDQTVLSISSGD
                     YPRSVIDFFINYEFLVPLMVRKS"
     gene            49893..50411
                     /locus_tag="IWO89_000654"
     CDS             49893..50411
                     /locus_tag="IWO89_000654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0455432.1"
                     /translation="MSTNKIAQLSLLSAACVAGRIAFQFIPNFQPMTAIFLFIVLYLS
                     LKDALIVMSLSIAISSFYFGVGPWVIGQWISYTVVLSLFSIVCLRKTVQRNLWLKGVC
                     FFLAGMVYGIGMTVFDTLLYRLPQPWIYYLQGVSFDLMHSIGNVVFFLVFLPVVKRFY
                     NHSGGKNEKNNL"
     gene            50392..50784
                     /locus_tag="IWO89_000655"
     CDS             50392..50784
                     /locus_tag="IWO89_000655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4430 domain-containing protein"
                     /protein_id="HAQ0455433.1"
                     /translation="MKKIIYSLLAMSVSILILTSCSTTYTQSASNQKEEVKTSQKATI
                     TLQENGQNFSEKEVVFKKGDDLLSILKRNFEVKEDNGFITSLEGHSQDEKNQLYWMFT
                     VDGKSATTGAKEIKLNDGQSVIFNLSKL"
     gene            50860..51795
                     /locus_tag="IWO89_000656"
     CDS             50860..51795
                     /locus_tag="IWO89_000656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HAQ0455434.1"
                     /translation="MENFKKSGMFSVIAALGANILVAISKFVGYAISGSAAMLNESIH
                     SIVDCSNQILLLFGDKQANKGQSELHQFGEGRAKYFFSTIVAMMLFFGGGALGVMEAF
                     EKLTHPSHEVGNPIIVIAILLFGLVIEGSSLRVAMKEIKELNTEKLSTMRFLRESRHS
                     EILIIFTEDLCAVIGLVLALAGTILTYATGIAAFDAISGLLIGFLLMGAAIFLAKEFY
                     SLIIGESVTASDLSKIKLAFERPEISRLIDIKTVHLSPTEILVAAKADIVGNKENEAY
                     SVINDIEKNMRQSLPDKKMYIYIETDKYDPNYSRK"
     gene            51928..52707
                     /locus_tag="IWO89_000657"
     CDS             51928..52707
                     /locus_tag="IWO89_000657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HAQ0455435.1"
                     /translation="MNKKKSAPWLQALHAVMPLCISYIPVGLACGVLLQKVGFDPLLS
                     GLLSILVFSGGAQFLVASMLTTQASFATTLLMVFFLELRYTLLGASLAGFMKKEKRTF
                     LAVFSQSLNDENYAVNYLKFSTDPSWNKRKALYVNWFSMTAWAGSNMIGNFFGSVIRV
                     DADLVHFALTAMFIFMFIMQMKSALLIFTGLFSGVLGVVFMVLFQNTFGLIAATVIAS
                     FAGFMLEKAFKKEKAKKLRDRGKDVNEPLFNFPEQEANHHD"
     gene            52700..53017
                     /locus_tag="IWO89_000658"
     CDS             52700..53017
                     /locus_tag="IWO89_000658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlD domain-containing protein"
                     /protein_id="HAQ0455436.1"
                     /translation="MTKLYLLLLVACLFIVAYIPRLFPMLYFTHRKVPSWFSDWMKYV
                     PVALFAALAFKDVFITHEHLDIAWNIKIAAMILVAGVAYKTRSMALSVLTGLASVFLL
                     SML"
     gene            53261..54871
                     /locus_tag="IWO89_000659"
     CDS             53261..54871
                     /locus_tag="IWO89_000659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HAQ0455437.1"
                     /translation="MNKKLMQGTFWLTFANLLCKVLGVVYLIPWLSMMGNNQDGMLAT
                     TLYNVGYLPYGLFLMLGTVGFPNAIAKKVAVATKEGDNAACRLIFRSTINIMFVIGIA
                     SAALMYLFAPLLAGISPIANVNNGILAIRSLCPSLIAIPILSAMRGYFQGKNDLRPYG
                     TSLIIEQAVRVIVILAGTYYLRVLTDGTILEAVLISTVASFFGGLAAIIHMYIVGRRK
                     DFFELKDFLISSRYFERENRSASVSIIRETLPFIFVGSVITIVQMIDQVTMKPLLHFF
                     RPEIADQQLEFLFSRASVNPNKLTLILISMVGTVAISSLPILSTMKKKDRIQIEKTVG
                     DSFSIALLILLPSLTGMSLLAGPLYTLFFGYDPESVGYFQMALLASLFFSLFTILSTM
                     LQSLNHHRFAIRLTVEAIILKVIFQVIGLGLVGGYGMSLSNGLAFGIVFVRGYHFMCK
                     EYRIAPLAKISNFFLKTFRSTLIMLAVCFAVFFLLNQRLSMESKSYAMIYCVAVGSIG
                     GLVFLFSQFGKNGFRMLKNVKHHSRSKE"
     gene            55028..55876
                     /locus_tag="IWO89_000660"
     CDS             55028..55876
                     /locus_tag="IWO89_000660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HAQ0455438.1"
                     /translation="MKLAVVTDSSAYLPERIKNHPDLFVIPIPLIMDGKIYNEGIDIE
                     ADEYYGLLKNSKDFPTTSQPVVGEVLGLYEELKEKGYDTILSIHLSSGISGFVNTLFA
                     MKDDISGVTVIPYDSKITSMPMGHMVEAALDLNDEGQSLDEILAHIDRIRDNTYAYLI
                     VDDLNNLVRGGRLTNGAALIGSLLKIKPILTFSEGKIVLFEKIRSSKKAFARAEEIIG
                     RRNNEIQPPVKLYVIHANNLEVAIAEKEKLQKKYPNAEIEIGHFGPVIGTHLGEKAIG
                     IAISAQ"
BASE COUNT        18536 a         9920 c        10899 g        16552 t
ORIGIN      
        1 aaaaaaagac gaaaataaac ggttttctat aaaaagaaga ccgtttattt tttgtcttta
       61 tttgtgtgaa aaacgaaaaa gagcacaaca catcaagctt ttattaattg tctgtctttt
      121 ataaatgcaa gtattaattc aaaaaaacag aagaaaaaaa caactacacc ttcttttata
      181 ctcgtaatcg tagaaaggag gcgaaaaaaa tgaatacatt acctatcaat acaggagata
      241 cagcattcat gattttatgt acagcaatgg tttgcctgat gactcctggt ctagcttttt
      301 tttacggcgg attagcacgg aagaataata tactaacgat catgtctcaa tcttttctct
      361 ccgttgggat aacaacgatt atgtgggtat ttggtggatt cggattggcc tttggacggg
      421 atatcggcgg tgttattgga aacccggcag atttcttttt gatgagacat gttacaagct
      481 tccccaacat gtttcatggt gcaacgattc catttttaat gttctttctt taccaactga
      541 tgtttatcgt gattaccgtt cctttgatga caggagcttt tgcagggaga ttgaatttaa
      601 aaggctatat tatacttgtt attttttgga atctgctgat ttatttccct gtttgccact
      661 ggatatgggg tggcggattt ttggatcaga tgggatttag agatttcgct ggtggagcag
      721 tcatccacac gacagctgga ttcggctctt tagcatgtat cctcacactt ggtcaaagaa
      781 agattccttt aggagaaaga agtcagcata gcagtcttat ggcagctgct atcggtacag
      841 gactgctttg gtttggctgg ttcggtttta attccggtgg tgcacttcga gcagatgaac
      901 aagcagtcaa tgcatttggt tcaacattca tcgctcttgc atttgcgatg attacttggc
      961 tgatcattgc aaaagtaaaa ggaaatggct ttgattttgt cgacgtacta acaggatccg
     1021 ttgctggatt ggcagccatt acgccttgtg ccggttatgt agaagcaaaa agtgccatgc
     1081 tgattgggat cattgcgggt atcgtctgtc atgctgctgt tgattttcgt aaaaagaaac
     1141 aatgggatga tgcacttgat gtgtggggtg ttcatggcat gggcggattc acaggtacga
     1201 ttctgatagg gatctttgca tctaacagcc atttactaac gaatccaagc agctggtact
     1261 tcttagggat tcaagtgatt ggtgtcatca ttactgccgt gtatgcctat gtgctgacaa
     1321 cagtgatttt aaaatcggcg atgcacttta caacgatcac tactacaaaa gaagaacaag
     1381 aagaaggatt ggactttttc ttctttggca acaaactcaa ttgaaacaaa ttcaaataga
     1441 aaagaggaag attcatgcaa caaagcaaaa cgaaaattcc tgcaaaaaaa ttaacgttct
     1501 ttggcttttt ggctatgacc atctcaatgg tcgtttcatt atatgaatat cccacattcg
     1561 cgacctcagg cttcagctta gtatttttct tgttattagg cggtttgcta tggtttatcc
     1621 ctgtggcatt gtgcgcagca gagatggcaa cggtgaaagg atgggaaaaa ggcggtgtct
     1681 atacttgggt cagtcgcaca ttgggaaaac gattcggttt tgccgcaatc tttttccaat
     1741 ggtttgagat aacagttggc tacttgacca tgctgtattt cttaactggt gcattatcct
     1801 atgcaacggg tattggtgct atacaaaaca ataaatatct gaaattggca atcctattga
     1861 ttattttctg ggctatttta ctctcacagt taaaaggaac caaatacact tcattgattg
     1921 ctcggatcgg ttttgtagct ggtatcttgt tgccagcatt agtcttgttt ggcttaggga
     1981 tccattatgt cgcttcaggc gctccactac aaacgatact gtctatgaaa acattgattc
     2041 ctgatttttc gaaattgcct acgctggttg tttttgtttc ctttatttta gcatatatgg
     2101 gcgtagaaac atctgccagt catgcaaatg aaatggaaaa ccctaaaaag gattatccat
     2161 tagcaatgtt tgtattggtt attctggcaa tcattttaga tacttttggc ggattgaccg
     2221 tcgctactac agtacctcaa catggtttgt ctcttaatac aggggtgatt caatcgttag
     2281 aattcctatt aagacacatt gatccaaatc ttgcatggat tgcgaaaatt cttgcgattc
     2341 ttgtttgttt aggtgtcatc ggagaaattg cttcttgggt aacctctcct tcaaaagcac
     2401 ttcacgtagc agcagaagaa ggattacttc ctgaatattt tgcaaaagaa aacacacacg
     2461 gtgttcctgc tcatttgatg atcgcaaatg ggatcgttgc aacaatctgg gcagccgtct
     2521 ttacattaag cggcgggggg aataatcttt catttcttgc agcgatgtca ttgactgttg
     2581 taatctacct gatgttgtac tttatgtttt tcgcgggcta tttgaagctg gtttttaaag
     2641 atgacagtct tgaaagagcc tatcaggttc caggcggaaa agttgtaaaa gtaatcgttg
     2701 cttgtttagg tcttttgact tcactttttg ctttttgtat ctcatttgtt ccacctgcta
     2761 gtatcgcagc tggacagcat gggatttatg aaacgattct gattgtttct tatcttatcg
     2821 tcttagttat tccatttacg atctacgcat gccgtcataa attccagcat gcagaaaaat
     2881 aaaaaatgaa agctggttat tcaaatggaa gtaaaagaat tagaacaatt gattggaaaa
     2941 aatttccact attatcaaac aggacaagtg gcggattaca ttccagcatt agctgaagtg
     3001 gatcccaaac aattaggtat ggcgatttat gatttggata caaaacaact gattgaggga
     3061 ggagacgccg atgtgcgctt tgccatcgaa agcatgtcaa aagttcccgt attgttatta
     3121 gcaatcaaag acaacggaat agaaaaagtc tttcaaacaa tcaacacgga accaaccggt
     3181 tttgccttca attctatcat gaatatggaa atcaatcatc gcaaacatcc aatgaatcct
     3241 tttgttaatg caggagcgat tgcaacaacc tcgttgattg ccggtaaaga tgcagatgaa
     3301 aaatttgagc gaatcttgtc atttatgaaa gagatctgtg atgatcctaa tatcacattg
     3361 aatgaagaaa tctatcattc tgaatcaaga acaggtgata tcaatcgctc tcttgcttac
     3421 tatatgaaag gtaatcaaat gatcgaagga gacgtaccgg agatactcga tgcttatttc
     3481 agacaatgct ccctcaatgt caccgctaca ggcatagcaa aattagctgc tgtgctggca
     3541 aataaaggaa ttgctccttg gaatggcaaa caaatcatta ctgaagaaag tgctacaatc
     3601 gtcaaatcaa ttatgacaac tgctggtttg tatgatgaat caggagagtt ttccgtccat
     3661 gttggtgtac ctgcaaaaag cggtgtaggc ggcgggttga tggcagctgt gccaaatcgt
     3721 tacggcatag gtgtcttcag cccggcacta gatccatttg gtaacagcgc agctggtatc
     3781 caattgttga aagatgtagt caaagaacta gacgctgaca tctttgaata accattcttg
     3841 aaagaggctg gggtaaaagt ccaaacggct caaagctacc tacaatgagg ctgaaattgt
     3901 gagcttgttt gacttttgtc ccagtttctt tttttattgg tttttgaaag ggcttttcta
     3961 aataaaaagg aaacaaaaat acgatttaca gatatgatta aataaaatca taagaaaaac
     4021 tggaggcgtg tatcaggaat gctggaaaat tacatcgagc gaaatatatt tagaaaagtg
     4081 tatctgtgtg aacaattatt tgaattccaa gaaatagaca tcgaacaaac agctatttct
     4141 ctaagggtga ctacaccaac gatccttcac gatctagaaa gtctagccga gtgtctagaa
     4201 tattgtataa aagaacaagt aagagagaag cataagtata aactcgtatt caaacatggt
     4261 attgctcttt ctgagctcac acagtttctg tacggtcaat cttatttttt aaagttttta
     4321 agttattatt ttaatggtat attcacgagt acagaactgg ctgatcttga atttatttct
     4381 ttgagcaaag tatatacaat caaaaaaatc gtattggatt tttttaaaga aaatgattat
     4441 ttgaaaaata aagaaatcat tatcccagaa tttgactcgc ggaacatctt gctagcttta
     4501 gtacgttatg tcaattggga aggctatgaa aataaaaatc cgtatgtaga agaagcttgt
     4561 aacgagttga ttgattatgt agagactcat ttttttaaaa gaagatattc ggaagatgaa
     4621 aagtccttta tcatacgagg aatagagatc gcaatcggaa gaaaggcatt tccccttgga
     4681 ctgaaagaag aagataagaa aagcgcagaa acgaagccgt tatttcaatt agtctgtcaa
     4741 ggattggaag agcaaaaagg aattctagac ttacaagaag aagatgtcta ttatattttt
     4801 tctttgttca acagtagaaa ttataccaat gagaacatgg agctgttaag aaaagatttc
     4861 gaagttgttt ataaaaattt tgttctcaat gacctatcac tgaatgaatt gattttacag
     4921 attattagta tcgctgaaat cgacgatgca gaaaatttta ttttcaagca atccttttta
     4981 caatttgtcc gtacgttgtg ggcagatgga caagtatttt taccggaaag gatctatctt
     5041 ctatcagaaa aagaacaact tctgtacgga aaaattgtag aaatattgga agagtggaaa
     5101 aagaaaaatc aaatccaact aagatggaat gaaaatttca ttcgaaaatt cgtcaaaagt
     5161 ctttctttga tcaacagtga acaagcgggg agacaggaaa tagaaatttt tgttgtttca
     5221 gaaagttcag tcaaacaatt tttttatcga acccaatttc atttgtatgc ggaagaaggc
     5281 atcgctgaaa taaatcctat tttatttcgt tcattagaag aattgcctga tgaatgtcta
     5341 tgtgcgaaaa aaagagtggt tttgtgcgat gctgctagct atcaaaatgg attagaaact
     5401 gaaaagacaa agatctatcc aatctcatta aaaggtgcaa acatccattt gtatcatatt
     5461 gctcaggaac ttcacctatt aaaaaaacat atttcttgat gagaccttaa ttaggagacc
     5521 ttctacaaag ctttttaatt gtctgtagaa ggtttttatt tgagtcctga ttcgtgcgaa
     5581 gatctactta tataacaagc tttctctgaa atttttttaa aatgctattg acttagagtc
     5641 aactctaagt agtagaatag ataaagtgta acaaatctat tttcattaaa agaagatagt
     5701 acggaggaga gaatgtatta tgagtacagt attacatgta ttggatgcaa tcaatagccc
     5761 cgcagatgtc aaaaaattat ctgttgagga aatgaaacag ttggcagaag agatgcgcca
     5821 aaaaatcttg caaaaggaca gcgatgtagg tgggcacgtt ggtccgaatc taggtgtagt
     5881 agaattaacg attgcgtttc attatgtatt tgattcacca aaggataaaa tcgtttggga
     5941 tgtttcccat cagtcttatg gtcataagct tttaactggt cgaaaaaatg gttttgaaaa
     6001 aggacattac cgtgaagtga gcggatattc caaccaacat gaaagtaaac atgactattt
     6061 tacagtgggg cacacatcta catctatcag cttagcaaca ggtttagcta aagcgagaga
     6121 tctgaagaaa gaatcaggaa atgtggtagc atttatcggt gacggctccc taagtggtgg
     6181 cttagctttt gaaggattga acaatggtgg tgttttacaa tccaatctaa tcgtattagt
     6241 aaacgataat gagatgtcta ttgatgagaa tcatggcagt ttatataaaa acttagctga
     6301 gcttcgtcaa accaacggta aaagcagcaa taacctattt aaagcaatgg gatttgatta
     6361 tcgttacgtg gataatggaa acgatctgga aacggtgatc cgtttatttg aagaagtaaa
     6421 agatattgat catccgattg tacttcacgt ccatacggaa aaaggtcgcg gttatcagcc
     6481 ggcaatcgat aataaaatga aatatcattg gcatgtacca tttgatctgt ccaccgggga
     6541 ggcaaagcaa gtaacttctg gagaaacatt taatatgatc atcatggatt atttggataa
     6601 gaaaatgcaa acaagtacag ctcctattgc cgcagtcaat gcagcgatac caggcgcctt
     6661 tggactgaaa acattccaag aaaaacatcc agatcattat tttgacgtgg ggatcgctga
     6721 acagcactct atcactttcg cttctgcttt ggcttctcag ggagtacgtc cagtcgtctt
     6781 ccaatcaagt actttcttgc aacgggcata tgatcaatta tcacatgatt tagcgatcaa
     6841 cgagaatcca gtgattattt tagtcggtgg cggaacgatc tcagctagtg accgaacaca
     6901 ccaaggaaca tttgacattc cttacttatc aagtatcccg aacatcaaat atcttgctcc
     6961 agcaacaaaa gaggatatga tctctatgct agattgggcg ttcgaacaaa cagcgcagcc
     7021 tgtagcaatc cgaattccta cacatggtgt agaacatggt acagctatcg caaatgatta
     7081 tagtattcca gcctatcaaa cagtaaataa tggaggaaaa gtagcaattt tagctttagg
     7141 cggttttctt gaattaggaa aagaaacagt aaaagctttt agaaaaaaag gaatcgaagc
     7201 aacgttgatc aatccaggaa cgatcacaga aaatgacgta gaaacattag ataaattaaa
     7261 agaaacacac gaattagtca tcacattaga agatggtagt ttgtcaggag gatttggcga
     7321 aaatatcagt cgttattatg gtgattcttc aatgaacgta ttaaattttg gtgcagagaa
     7381 agaattcacg gatagcgttc ctgtagggca gctttatgaa cgttatcatt taacaccaaa
     7441 acaaatcgtt gaagatagtc tgcgtgtctt gaatattcaa taattcatta tgctcaccat
     7501 ccagataatt gtttatacta gtatctggat ggttttttca ttataaaaag caaaggggtg
     7561 gatgttatgt atcagcaagt agaagaatat gtcaaaaagt tccaagaaaa atatcctagt
     7621 tatcaatcag aaaaattagg tttcttttca tttggggata cagaaaaaat ggcagacgag
     7681 ttgagcgaac tagttgtaaa aaagataaag actggtacat caagtggtta cgatctgtat
     7741 aaacaagaga atgagccttt accaaaagtt ggtcaattag acgtggtcct gaatggaaaa
     7801 aatgaaccag tttgtatcat caaaaatata aatgtgacca tcttgccttt tagtgaagtc
     7861 acgaaagagc atgcttggaa agaaggagaa ggggatcgga cattagctta ttggcggaaa
     7921 gtccatgctg agttttttaa acatgcttat aaagaagcgt tgaacataga atttacagaa
     7981 gagaagctgg tcgtatacga agaatttgaa gtaattttct tttagtaaga aaggtctgct
     8041 gaaagaaata gctcattatt gtctcctgaa tagaaaaggg ttacattttt atatgaagct
     8101 gtatttttat ataacattac tggaggagat attgtggata cactggaaca aatcaaaggc
     8161 ggagtcgtcg tttcttgtca agctttggaa aacgagccgc tacacagttc ttttattatg
     8221 gggagaatgg ctttggctgc aaaagaaggc ggagcagttg gtattcgagc aaatacgaca
     8281 gaagatatca atgaaatcaa gcgaatcgtt gatttaccgg taatagggat tatcaaaaga
     8341 gattacgatg ggtcagaagt gtttattact gcgacagaaa aagaagtgga agaacttttg
     8401 gagacgcaag cagaaatcat tgcactggat gcaacttctc gtaaacgtcc gacagaaaaa
     8461 acaactgctg aacttgtcca aatgattcac gaacacggtc ggttagctat ggcagatatt
     8521 tcgacttttg aggaagcgct tcaagcgcaa gaagatggtt ttgatctttt gtctacgact
     8581 ttagcaggct atacagaata ttcacgaaag acgcagacac ctgattttga tttattgaat
     8641 caaatcatag aacaagtcga tatcccagta attatggaag gacatactga ctctcctgaa
     8701 caagtagaga aagcatatga attaggtgct ttttcagtcg tagttggatc tattatcacc
     8761 cgtccgcaat tgatcactaa aagatatgtc gaagccgcaa gtaaagcaaa taactaaaaa
     8821 acacgcacgg cacagattct tgacaaaaaa gaaaaattga tagatagttt aaagcagatt
     8881 ctaagaagat gaaaaaagca ttacttttca ttttgtcttc aagatctgct ttttaacgaa
     8941 ttttatagaa agaaggcgaa accatgtttg aacggagtcc ttcagaattg acagaacgtg
     9001 aaaattacaa gttgctgatt ggttcagtca tccctagacc agtagcagta gtaacgacta
     9061 aatcagcaaa agatatagtg aatatcgcac ccttcagtta cttcaatatc gtcagttcga
     9121 atcgacccat cttatccttg gcgatccaga gaaaacaaga tgcagtaaaa gacacagcaa
     9181 agaatattct tgagacaaga gaagctgtta tccatatcct tgatgaggac aacgtagaag
     9241 aagtaaataa aacagcagcc aatctgccta atgaggaaag cgaactgtac ctgactgact
     9301 tgactttaac tgattcagta atcatttcag taccagcatt gacagaagca aaagttcgtt
     9361 ttgaaacatc cttatatgaa catgtcgaga tacggaataa aaataaagta acagctgatt
     9421 tatttttgct agaagtacag aaatatcatt taaatgaaac agtctatgat gataagacag
     9481 gaaggatcga tccaagaaaa ttgaatgctg taagccgttt ggcaggaaat gattatgcag
     9541 caataggaga aattttcacg attgcacgtc ccgaataaaa aggagcacta gttatgttaa
     9601 aaataagcta taaaccatcg acagattcaa aagaaatgaa aaaagaatat gaaacagtga
     9661 atgattttct gcaagggcag tacttagaag ttccgccatt gcaagaccat tttgtcgtta
     9721 ctactgttac tttagatggt aaggaaatag aaatgccaga tcaaacgatt agcggattgt
     9781 ttaattactt taacaaataa atatttattc cgaaaagtga aaaaacatat aaaaaaacac
     9841 ataaaactaa aaaattatca aagaatcctg gtgcaactga aagaatttgc aacgggattc
     9901 tttcaactta tttgttattt ttttctaaaa aatattaaag attgttaaat ttcactattt
     9961 catttcaaat tccattgcct aatcattagg aaaagtctat catgaactcg taaatagaat
    10021 atagtaataa ccttgttagg tgaggctcct atacaaacat aggctgttgc ccagaaacgt
    10081 cgaaagtcgc caatgggtaa aacagaaact gttggattaa ggctttttcc aatagctaag
    10141 aatgttttct ttacgttgta tagtgctaaa actcggacga tgagatgatg ctaggtcaga
    10201 attcttgttc gaaaaggttt ctgacctttt ctttatattt ttgtgactga cttatttgat
    10261 gagacaaact cgctcctcac ctgacaagtg gattgctgtg tgaggaggag atgcggtgaa
    10321 aaaagatcga ttggtggagg tcgctcggat tcacttctgg ttgcacaata caaccagaaa
    10381 acacaacaag caaaactgat ctctatcatg agagattcat atgtcgatat tccaggttac
    10441 ggaatgaata agataaatgc agcttactct tatgggggag ttgatttatt aaatcaaaca
    10501 ttaaaggaaa atttcaaatt tgaagctccc tattatgcaa gtatcacatt tcaagatttt
    10561 atcgattgcg ttaatgaact gtttcctggt ggagtcaaga tcgatgcaga aaaatctcta
    10621 gatttagatg gcgtatatat aaacaaagga gagcaagtga tggatggtaa tactttactc
    10681 cagtatgctc gatttcggga agacaaagaa ggagacttcg ggaggatcag aaggcagcag
    10741 caagtgatca aagctatctc tcagcagctg aaagacgtaa cttcaatatt caagctaccc
    10801 aaagcggttg ggaaattact tggtagtatc caaacgaacc ttccagaaag tgtgcttctg
    10861 gattgtggta tggatttcct caaagacata tgttagaata aacaggtata aatagtgaaa
    10921 taaaggaatg acaagcaaga gaaggaggaa aaaatgaaaa gaagtgacaa gcacggcaaa
    10981 aatcgaacag gcgcttatat tgccggcgca gtgatcttaa tagcagctgc gggtggcggt
    11041 tatttttact accagcacta tcaagaaacc caagcagtag aagctggaga aaagacagtt
    11101 gagcaatttg tccaagcttt gaacaaggga gattataaca aagctgcaga aatgacatca
    11161 aaaaaggcgg caaataaaag tgcattatct gaaaaagaga tcttagataa ataccagaat
    11221 atatacggtg ctgctgatgt caaaggactt cagatatcga atctaaaagt agataaaaaa
    11281 gacgattcta cttacagttt ttcatataaa gcaaagatga atacctcatt aggtgaattg
    11341 aaagatcttt cttataaagg aacattagac agaaatgatg gtcaaaccac gatcaactgg
    11401 caacccaact tggtttttcc agaaatggaa ggaaatgaca aagtaagcct gaccacacaa
    11461 gaagcagcaa gagggaatat tatagatcga aatggtgaac cattagcaac aacaggcaaa
    11521 ctaaaacaat taggagtcgt tccaagtaaa cttggagatg gaggcgaaaa aacagccaat
    11581 atcaaagcga ttgcttcctc cttcgactta acagaagatg ctatcaatca ggcgatttca
    11641 caaagctggg tacaacccga ttactttgtc ccattgaaaa tcattgatgg agcaacgcca
    11701 gaacttccag ctggagctac cattcaagaa gtagacggca gatattatcc tttgggtgaa
    11761 gcagctgctc agctgattgg ttacgtggga gatatcacag cagaagatat tgataagaat
    11821 ccagaattaa gcagtaatgg aaaaatcggt cgctcaggtt tggagatggc ttttgataag
    11881 gatcttcgtg ggacaacagg tgggaaatta agcattacag atgcagacgg tgtagagaaa
    11941 aaagttctga tcgaacatga agttcaaaat ggcaaagata tcaaattgac aatcgatgcg
    12001 aaggcacaaa aaatagcttt tgacagtcta ggaggaaaag ctgcatcaac tgttgcgaca
    12061 acgccaaaaa ccggtgatct tcttgcgctt gctagctctc caagctatga tccaaacaaa
    12121 atgacaaacg ggatctcaca agaagattac aaagcttatg aagaaaatcc tgaacatcca
    12181 ttcatcagcc gatttgcgac aggttatgct cctggatcta cgtttaaaat gattacagca
    12241 gcaatcggtc tcgacaacgg cactatcgat ccaaatgaag tgttgacgat caacgggctt
    12301 aaatggcaaa aagacagttc ttggggatct tttaaaacaa cgcgtgtcag tagtgatgta
    12361 tcacaagtag acttaaaaac tgctttgatc tattccgata atatatatgc ggcacaagaa
    12421 acgttgaaaa tgggtgagaa aaaatttcgt acaggcttag ataaattcat ttttggtgaa
    12481 gaccttgatt tgccaattag tatgaatcca gcacaaattt ctaatgaaga tagctttaac
    12541 tcagatatct tgctagctga tactggatat ggacagggcg aacttctaat tactcctatc
    12601 cagcaagcag caatgtattc tgtttttacc aacaatggca cacttgtcta tcctaaattg
    12661 attgcagata aagagacaaa agataagaag aatgtcatca gcgaaacagc agtacaaacg
    12721 atcgtgccag atctgagaga agttgtgcaa gatgtaaatg gtacagcaca ttctctttct
    12781 gctttaggga ttccgttggc agcgaaaact ggtacagcgg aaatcaaaga aaaacaggat
    12841 gtaaaggggc aagagaacag tttcttgttt gctttcaatc ttgataacca aggttatatg
    12901 atggtcagca tgttggaaaa taaagaagat gatgattcag caaccaaacg agcatcggaa
    12961 ctattgcaat acctcaacca aaattatcaa taaaaaattg ctttttaagt acagtaaaag
    13021 gactgtggcg ttatcgtcac agtccttttt tcaaataaac ggttttcacg atgtctcttt
    13081 cacaacttct ttttgattta ctcacacttc ataaactttt tttgtaaaat atttgagaac
    13141 aagtggagaa ataagtgtac ttaataaaat cacgataact aaaggtgaat agtactgtgg
    13201 ttcgatcaat tgactttgct gtccaatttg taaaatgatc aaagccattt ctccacgcga
    13261 aatcatacca gcaccgacca tcaatgaact atgttgagaa aaaccggcaa tctgagcacc
    13321 aaaataacct ccaagcaatt tggtcaaaat cgctacaagc gtcaatatag aaataaagag
    13381 tagttgttca ctaaagtcag aaaaatcaac ttctaatcct acactgacaa aaaatactgg
    13441 tataaatact gcatatccta aagcctctac attgtggtat acttcttctt taacctttgt
    13501 ctgactgaca gctatcccag caaaaaaagc accaatgaca gagctaagcc caacaagatc
    13561 cgctacgtaa gacattccta aacaaataac tagcgatacg atgataatag aagaatttgc
    13621 gtacactttt tctgcaaatg aaagtaggaa aggcgcaatc catttgacta ataagaaaat
    13681 aaaaacaaaa taaattagct gttcaatcaa aacaagcggt aaagaagcat tgctagaagc
    13741 ttcccccgtt agaaaagata ggctgaaact tagaactaaa acgactaaaa tgtcatctac
    13801 cacagaagca cccaaaattg ttgatccttc ttttgtattc actacgttta gttcttttaa
    13861 tacttctact gaaatactga cagaagttgc cgcaagaata atcccaaaga aaatcgcttc
    13921 atttcccgaa acttggaatg ctttgcctgt caaccagcca aaaacaaccg ggaagatgat
    13981 cccaagcaaa gcaacgaaca taccaggtcg gaaatatttt ttcaaaagag ataaatcact
    14041 ttccagacca gctaaaaaca ttaataagat cacaccaatc tccgaaaagt catggactag
    14101 aatatcaggt ttgacccaat tcaatccagc ttggcctagc aaaacaccaa caagtaattg
    14161 tccaatcact gctgggatac ctaatctccg cgagatatgt gagccaatcg ttgttgaaat
    14221 caatatcagg cataaaatac caataaattc cattctcatc attcctttgt ataaaatata
    14281 gcccgaactt agcataacat acattattga aaaaatagga aaatatactc acaaaaaaac
    14341 caacaatcca aaagattttt ttggattgtt ggtttttata cggtctattt taataactaa
    14401 aaatgattaa cgtatagtca tttttcttat tctgatgaaa ttgataatct cctggatgcc
    14461 catgaaaatc aaaataaagc cgaatatttg gaatagaagc atcaaacttt tgaatggatt
    14521 gaacaataag acgaatccgc caatcaacaa cagaatacta ctataaacac tccaactttt
    14581 agcttttgta ttcatgctga agaacaactg gaacaagctg ttgatgataa cagctaatcc
    14641 aagaagaatc ggcaggatag aaacgattgc aggtgcaaaa gcatagacga tcagagctaa
    14701 gatcaggaaa aaaagaccac tgaacagacc gatactccaa gaacctgttt tccttttaaa
    14761 acggtaagcc tcaaaaagat tcaacaaccc aagcaaaaca agataggcgg tgatcagata
    14821 accgataaaa tgaaaaacag cagaagggtt tagcacaatc gcaatcccag aaataatata
    14881 aacagcagct cttaataagg cgtatttttg aatattttgt ttaatcgtct caaacatata
    14941 agaaaacctc ctttgactta ttttaacgtg aactagtcat tttaccaaat taaatgttac
    15001 aaaatgagcg aaggagaaga atatctctgg aaaaatcaac ttctgaggat cttttccatt
    15061 tttttgcctt ttgctagctc atcgatcatt ttatctaaat aacgaatttc tttcatcaag
    15121 ttatcctcga tgtcttctac tcgtacacca caaacaacac ctgtaatcaa ggaacgtgaa
    15181 ggattgggga caggggcttc cgcaaaaaat tgttcgaaag tcacttgttt ttgtagttga
    15241 tattctagtt ctgtttggga ataacccgtc agccaacaaa tgatttcatc tacttcctct
    15301 tttatacgat cttttctctc cgcttttgct acatacatag gataaacgct tgcaaaactt
    15361 gtcgtaaaaa tacgattttg cgccataatc agtcaccttt ctttttttca aattatttac
    15421 tacttatagt atataagaaa aagagaagaa aaagcgatta ttcaaaaaaa caaaaaggat
    15481 tgtgacaaaa ttcttgtcac catccttttt tccgctctct gactttttac aatcttcata
    15541 gctcaagtgc gcatcagata atcgaatgct cctaaagctg cagtagctcc tgaacccatt
    15601 gaaacaatga tttgtttgta tggactatct gtacaatcac cagctgcgaa aacgccgggg
    15661 atattcgttg cgccattttt ctcggtcact atttcatttc ttggattcat ttcgatcgtt
    15721 ctatctagcc aatccgtatt agggagtaag ccgactagaa tgaatactcc tgaaacagct
    15781 agtgtatgca tctccatcgt acggcgatcg atataggaga gagattctac ctgattcgta
    15841 ccgctgattt ctttcgtcgc tgcatgtttg ataacagtga cattcgcaag agagactagt
    15901 ttatcctgta agatttgatc tgcttttaat tctggtaaga attctagaac atatacatgt
    15961 tttgctaacc cagccaaatc gattgctgct tcaacaccag aattaccacc accgataaca
    16021 gcaatctctt ttcctttaaa taaagggccg tcacagtgtg ggcaataagc aattccttta
    16081 tttttaaact ctttttcgcc tggtacattg atagaacgcc agtgcgcacc tgtcgcgatg
    16141 atggcggttt ttgctttaag tgatataccg ttagctaagc caatattaat aaaatgttgc
    16201 tctttattta aggaaacggc ctgttgattc atcataatgt cgatagggta actacgcaca
    16261 tgactttcta cttgctgcat caattttgga ccttctgtgt aagaggtacc aatcatattt
    16321 tcaatcccta gtgtttcaac gacttgtccg ccgaaagaat cagcgacaag tcctgtacgg
    16381 attcctttac gtgctgcata gatagctgca ctactagctg ctggtccacc gccaatcact
    16441 aatacatcat aaggttcttt ttcaaacaac tcttcttcaa tcaaagggcc agtgattttt
    16501 tcaacaattt gttcgatcgt catgcgtccg ctactaaatt cttctccatt taaaaacaca
    16561 gttggcactg ccatgatttt tttatcatcg atttcttttt ggaacatccc accttcaatc
    16621 atcgtatgag taatatttgg atttaataca gctaatatat tcaatgcctg tacaacgtcg
    16681 gggcaattat gacaagtcaa gctaacataa ctgtcaaaat gcaatgtttg tgttattttt
    16741 ttgattttaa gactcaaaga ttcactgatt ttcggtgccc gtccactaat ttgtaacata
    16801 gccagtaaaa aagaggaaaa ttcatgaccg agggggatac cagcaaaaac gattccactt
    16861 tctttgccaa ctggattgat agaaaaacta ggtgtgcgca tcgaatctct tttttcccaa
    16921 gagattttag gagacatttc tgctatttcc ttcgtaaatt cagctagttt ttgactattt
    16981 tcactattat ccaaactaac agaaaaaaca ataggagatt caatcagtgc taaatattgt
    17041 tcgagatttt gtttagtttc tttgtctaac atatccacca cctaaatctt tccaacaaga
    17101 tcaaaacttg gtttcaatgt ttcgccactt tctttccatt ttgccggaca aacttcacct
    17161 gggtgggtac gtacatattg agcagcgcga atcttatcaa tcagtgcact agcgtcacga
    17221 ccgattccat ctgcattgat ttccatagct tgaacgattc cgtctggatc aataataaat
    17281 gttcctcgct gagctaatcc ttgctcttca tccaaaacat caaaaatacg agaaatctga
    17341 tgagacggat caccgatcat cacgtattcg attttaccga tagcatctga tgtatcatgc
    17401 catgctttgt gagtaaagtg ggtatcggta gagcaagaaa agacttcaac acctaaagat
    17461 ttcaatgtac tgtattgttc ttgtaagtct tctaattcag taggacaaac aaaagtaaaa
    17521 tcagctggat agaagcaaag aatactccag ttccccataa gatctttttc actgacttct
    17581 aaaaattctc cttgatgata agcatgtgca ttaaattctt cgattttttt cccaatcaat
    17641 gacatagtaa aacctccaat aataattatt tatttataat tattgtaatt tagttggagt
    17701 tgcaaagtca atggatattt tgttatttaa taaagattac tcaaaaacga aggttaatta
    17761 taaaaataga agaggtcgca agaaaatgct actgctggaa atactcggga taacgttgag
    17821 taacagtttc ctgttctttc agcatcatgt gtaagtgcaa tcccgtttga aatcgagctt
    17881 cttctggttt tttatcaact aaagccttga gtaaatcata atgttgttcc atgatcgttt
    17941 cccatggaag cccctgaatg gtcactcgta accagcggaa acgttctaaa tgggtattat
    18001 ggtcatctaa ccagttccaa acttcttttc ttcctgcaat ttcgaaacaa gcttcatgaa
    18061 ataaattatc tgcacggaaa aaagcgcgtt cgtcatttat tttgactgct ttttcttgtt
    18121 cttctaaaat cgtttctagc actattcgtg tttgtgtgtc caatttggaa caacattcaa
    18181 tcatgatttg ctcttccaac tgttctcgga caaagcgagc attttctgca gaaacaagat
    18241 taattttgga aacatacgta ccactttgag gtatcgtata cacgagttct tgctgacgta
    18301 gttgaatcaa tgcttcgcga atgggagtcc ggccgatagc aagatgttcc tccagtgcct
    18361 tttcagatat tttccttcca ggttctaagt cagcatagat aatttgttta cgaatcgctt
    18421 gataagcttg tgattgcaag ctgcttttca tctttattcc tccaaaatca tttctatgaa
    18481 cgtagtataa cagaagaggt ctgaaaaaca atctggtatg tcacattaac tagtaaattt
    18541 gtataaatag taaaattttg gttgacatag cgatttttat tgcataaaat tcacttgaat
    18601 acaaaaacta atatgttagt ttaggagggg aaaaatgatt gatttaaaca actggaggaa
    18661 ctttatccct gaattatctc aaaaagaagt agaaatacct cattttgacc gtgcatcatt
    18721 aagagaaaat ggacaaaaaa atcctgtctg gatccatttc ggtggcggaa atctttatcg
    18781 aggatttcat gcagaaatcg ctcaacgttt agcggatcta ggcgaattgt ccactggtgt
    18841 cattgtttgt gaaacattcg atgaagaaat aattgaaagg gcttaccaag attttgatga
    18901 tttgattctt gaagtggtca tgccaagcag tggagagttc gtcaaacgta tattagctgc
    18961 gacagctgac agtctgtatt gtcatccaga ttgttctgaa cactttgata agatgattcg
    19021 acactttgaa aatcctagtt tacaattagt tacttttgct attactgaaa aaggttatcg
    19081 attgaaagat acaaataata aatatatagg accgattgca gtcgatataa aaaatggtcc
    19141 aaagaagcca tcacatacaa tgagtattct tgctgtgcta ctttataaac gttttttatc
    19201 tggagcttgg ccgattgcac ttgtctctac agataatttt tcacaaaacg gtagtcaatt
    19261 acgtacttct ttgctaacga tcgcaaaaga atggcaaaag gaaggttatg tacaaaatac
    19321 atttatccaa tacttatcga atgaagaaat tgttgcgttt ccatggacga tgattgatcg
    19381 tatcaccccg aatccttcct tgtcagttag caaaatgttg gcaaaagagg gcttttcaaa
    19441 tatgtcgatt ttccaaaccg caaaaggaaa taatattgct ccatttgtca atacagagga
    19501 catccattat ttaatcatgg aagatcattt tcccaatgga cgtccgaaat tcgaaaaagc
    19561 tggggtattc ttgactgatc gtgcaacggt agataaagca gatacgatga aggtgacgac
    19621 ttgccttaat cctttgcata cgtctttagc tattatgggt tgtttactgg ggtataggag
    19681 catagctgag gagatgaaag atccagattt atcgctgcta gtttctgaaa tcgggtacaa
    19741 tgaaggcctc ccagttgtgg agtctccagg tatcattgat ccaaagatct ttctggaaga
    19801 agtaattact gtgcgctttt ctaatccaaa tattcctaat atgcctcagc gaatcgccac
    19861 ggatacctct caaaaggtgg cgattcgtta tggtgagacg attaaaaaat atatggaaat
    19921 agaagttaaa gacccgcaga cacttgtatt cataccattg actctcgctg cttggcttcg
    19981 ataccttatt ggcattgatg atcaaggaaa accattcatc ccgagtccag acccactatt
    20041 gacagacctt cagcataagc tttccgatat tcaactgggt acgcaagata catcacatat
    20101 tcatgagaaa atggctccta ttttgtcaaa taaagaaatt tttggtgtag atctttacca
    20161 agcagagctt ggcagaaaaa tagaagaaat gttcatcaag atggtagcag gaccaaatgc
    20221 cgtacgaaaa acattacagg attacttaca agaacatgga ggaaatttat catggaaatg
    20281 acttttagat ggtacggatc acaggaagaa aagattacct tagatgaaat cagacagatt
    20341 cctgcggtca aagggattgt aggcacattg atggatatac cagtaggaga ggtatggcct
    20401 agggagagga tcaaagaact aaaagaagaa attgagtctc gtgggttaac actaaaagta
    20461 attgaaagcg tcaatgtgca tgatgccatt aaaattggat taccttcccg tgagcagtat
    20521 atcgaaaact ataagcagac aatccgaaac ttggcagaat atggaataga agtcatctgc
    20581 tataatttta tgcctgtatt tgactgggtg aaatctgacc ttgactaccg cttagaagac
    20641 ggctcatcca cgttagcttt tatatctgat gatattccag atgatccaag agaaatcgtc
    20701 gcgcgcatcg aacattccag caatgaattt ggattgccgg gatgggaacc tgagcgctta
    20761 gctcctatca aatccttgct tgaagcctat gcatcagtag acgaagaaaa acttcgagaa
    20821 aattttgcct attttctcca atcaatcatc ccaacatgtg aagaggtagg ggtcaaaatg
    20881 gctgtacatc cagatgatcc gccatactcg atttttggat tagctcgaat cgtaaagaat
    20941 cgagaagatt tagattggtt gtgtcgcata gtagacagcc cttataacgg aatcactctt
    21001 tgtacaggga gtattgcaga agatccagaa aatgatgtat atgggatttt agcagaattt
    21061 tgccaaagag atcgaatccc atttgcacat gttcgaaata ttaaattttt agctaatcga
    21121 gatttctatg aggcaccaca taaatccgaa tacggttcat tagacatgta ccgcatacta
    21181 aaaacaatgt atgacaacgg atttaacgga tacatccgtc ctgatcatgg aagaatgatc
    21241 tggaatgaga caggaagacc aggatacggc ttatatgatc gagcattagg tgctgtttat
    21301 ctaaatggat tatgggaagc aatcgaaaaa ggaaacaaaa gtaaagataa ttagtgaaaa
    21361 aaactgtaat tttattatgt aaataagaag gatgttcaaa ttcttgatac ataaatccgg
    21421 aggttgttaa taagcattcg gatttatgta tcttatttgg tctaggaagt aacttgattt
    21481 tcttttttcg attgatccat gagtgcttct tcttttaatt caaagatata ttctgagatc
    21541 ctcataatca cttttttcca gtcttctggt gtgatgattc cttttaaaat atagattttt
    21601 ttgatatgag aaggcaattg tttcaaaggg cagtcgctga taacgatgtt gcagttctcc
    21661 ataaaatcat tttgtatttt gatcgattca tttgaaaaaa tttgttctat cttaacgatc
    21721 agatctttac tgatataaaa atcacgcgta tagttcacac caatcacaat tcgcggctgt
    21781 tttttgtagc gatcatacaa atgtactagg tcttcaacca tccattgcac attttccttt
    21841 tctaaaataa ctggtaacaa gtgttgattt tgttctctga aatccatata aaattgttca
    21901 atggcggttt ggacttcttt caatacgcta tgttcttgat aaagcatgaa agtaggaagg
    21961 ggcgtatttg gttgattgaa gttaagaaat ccattataaa gtttatttcg tgcgaatgcc
    22021 aatgagtaaa gaatcttttc tttttcaggt ataaacaaat caaatacagt acaaagggac
    22081 tccaccattt ctcttgaata aagaacatga ggaacgtttg ctttttttaa aagtttgtat
    22141 tgctccaagc ttgtttcttc cgattcgatt ttcgtgatag aaagtcgagc taaaatattc
    22201 actaagatgc gttgttcgcg acaaattggc agattaggtc gcaaaatatc tacagagaga
    22261 tcaataaaaa agtcaatata ttcatactct gtgaattctt tttgtacgtc tttcacagaa
    22321 gttgcaggaa aattctcttt acatattttt aatagaaaat ataatttatc tatcataccg
    22381 ctatttagtc ggtcaagtac ttctttttta aaaaaagtat tcatcataaa agccactact
    22441 tgcggatctt catcataaaa ttctgtagca gtacttgaaa agatgttcca ataaatatct
    22501 aatgctgcat attgaatttg tacctctttt ccagaaaaag actttctgcc attattagaa
    22561 aaggagatag acaaaccgta ctatgctaaa aatgcattaa tctttttcat tttcgtatac
    22621 aaataagagg cactatagtt tgtttgctga ctgaaactga taatcgaatt tgtctgacag
    22681 ctgaacaaag aagtcaagat ctgataactg actgattcgt tgcagtatct tccaaataga
    22741 cgataatagc agtaagccgg atcaattcct tcaaaattca actttaaaaa aggtttttgc
    22801 ttatcaatat gcagttctgt tagattgaga cgctcaaagt cttgttctaa ttctttgatg
    22861 tatagtaaaa aatttgattc agacatatgg aaatgatctg ctaaatattg tgtagtaata
    22921 aagaaacgat gttcagatag gaaaagaaaa atatctaatt ttgtattgct ttttttcttc
    22981 aagataaacg ttttaatcgc ttcttgagtt tgcatgaaag aggctccttt ttacttaaat
    23041 tgttaaaact tgaaaatatt actgtttttt tcataaattt agacaaaaaa acagtaaaca
    23101 tataggtaga atctctacta tcttatttct tgcatacaaa aaaatcaagg agtgagagag
    23161 ttatgagata ttcaaccatc ttaatatcaa catttgcagc cttaagctta tcgcttacta
    23221 taaatcagac agtccacaca gaggagacag aaacagccaa atggacagaa accaagtggg
    23281 ccaacgtcaa tcgaattacc tttcaggatg aattttcatc cttcaattca ggagtcgcaa
    23341 aattaagaaa aggacaaaca gcgaccatca aagcaaaaat tgactattcg ggagacttag
    23401 cacctatact acaatcagct gttacatttg agaatgttga tcccactttg tcgattggct
    23461 atgacgagaa cactttgact atccaacaca agcaagcgat atttaccttc accgttacat
    23521 taaatcagga cttgttaaaa ccagcgcttt ttactgtcaa ggtatcagat ggactaccga
    23581 acgatcatca tttaacgccg tatagtcagc ggcagacaat tgagcaagat tcagccatcg
    23641 actcaggagg agatcttgtt gaagaaccaa cagataaacc agaaaatgaa aataagccag
    23701 aagtaccgcc tacagaaaat ccagacggag aacagaaacc ggaaatagaa cctggagaag
    23761 aacccgatac agaaactcaa ccagagccag acaataaatc aaaacctgaa ataacccctg
    23821 gagaaaaacc agatgtaact ccagaaccag atacggattc tggaaaccaa acagtaccgg
    23881 aaacaaatcc agacacagat aatgaaactg aaaatccaga aaaaccagag gtagatccag
    23941 aagaaaaacc agatgtgact ccagaaccag atacggacgc aagagaccaa ggaataccag
    24001 aaaaaataaa caaaaaaaca attcaagagg atggaaaaaa agagagtaaa aaatcaaatc
    24061 tagcaattct aaaaataaat gaagagcagc tgaataaaaa atcacgaata tttgatagcg
    24121 cccaatcagc tgaaacctta aaaagtagca aggatacaac ctttgctgtc ctgaaacgaa
    24181 aaataaacag ctaccaaaat ccggagagtc acaaaacaaa gttatactgt ggagcggaat
    24241 cattctatta agtatagcaa caatgctttc tgcaaaaaga tttaaacaaa acagatctct
    24301 ctaacatttg aataatgtct gtgaacgcat ttttcaaata aaaagaaata tccagaaaat
    24361 gaccccaaaa taccagattg aaatctgata tttggggtcg ttcatacttt atctttcttc
    24421 atatttattt taagggatat ctgtttcttt tggaagatgg ttcgggatct ttttcacttt
    24481 caatacatct aaaaagaagg taaagatcgg aatgccgaca atcaagcccc aaacgccaaa
    24541 gaaccgttcg ctaacaagta agaccacaaa tgtataaaaa atcggcagtt ctgttttgct
    24601 ggacatcaac ttcggattca gcacataaga ctcaattaga tggacaatca aaatcgtaat
    24661 caggatataa acaacatctc ggatgcctcc ttgggaataa gctatgaaag atagaggaat
    24721 acaagaaatg ataacacctg ctactgggat cagacttaaa atgaaaatca tgatcgctaa
    24781 actcagcagc tgatgaaagc caaatacagc caaggcgatt gtagtgagca acgtatttaa
    24841 aagagcgaca acaaattgag cttctaatac caaaccaaat gtattgacaa acttatcggc
    24901 aaaatagtaa atatcttgaa agaaccaaga aaattcactt tttaaaaaca atcttgagaa
    24961 tacaattgtt tttttctttt caatcataaa aaagaaactt aagatgaatg acatagcaaa
    25021 cgccactgcc atttttccta tatcctgtaa atagccaagc gcaatcgaag cgccattttg
    25081 aagctgttca aaaaaatcgt ttcgctcaat ataagtatgg atatattgta aaacaggatc
    25141 tgcatctttt ggctgatgct cgtaaaaatc aataacggac tggatcatat caaatgtttg
    25201 atgaataagc atcgggatat actttgtgaa tgccagatag ataaggtaaa cgatcagtgt
    25261 ataaatcatg atagctaaaa cacctgtagg gattcggaca aatcgctgaa tgatttgaac
    25321 aagatgaatc atcaaataag taaagataaa tgtgagcaaa atggttgtaa tcatacttct
    25381 aactaagaaa agaacaaaaa cgacaaataa taaaacagtg agacggcgta ttttttcatt
    25441 cgccaaaaaa cgctgataaa cggtcaaatc gacaacctcc aaataacaaa aaataaacac
    25501 gttattcatt atatgatgga atatgaaaat aagaaagaga aagagcaaaa agaaaaaccg
    25561 ctaaatgacg ttatcttatg agttggtttt cctcataaaa tcgttcatct agcagtttca
    25621 gccctttttc tggtcttgat gaaaattatc tagcattttt cgggtcagat gttgcagtgt
    25681 atccaattct tcctgttcaa gtggaagata agacttgatc ttttgtggaa taccttctgc
    25741 ttgtttttgc aacgcagcac cttgaggagt cagcgtaaca tttacgattc tttcatcacc
    25801 cgtttcacgc tgacgattaa tataattttt ttctgctaac ttttttagta agggagtcag
    25861 cgtaccagaa tccagacaca aatcatttcc tagctctttc actgaagtct ttttcttttc
    25921 ccataaaact aacatcacaa gatactgggt ataggtaaga ttcagcggct tgaggaggga
    25981 agtgtagtgt ctaatgattt ccttagaggc ggcatataaa ggaaaacata cttgattaac
    26041 taaatataga tcttccattt ttccacctcc gctgttttac tatagtgtat catagcggat
    26101 ttacacaaaa aagtctattc actaactgct ttcagagtaa cttctatatt tccagctgta
    26161 gcttttgaat acggacacac tttatgcgct gcctctaata attcttgcgt ttcttccaga
    26221 gaaagacctt cgatagatcc ttcaatctct acacctaaga caaagctcgt ttcactttct
    26281 gaataaaggg aaacttgtgc actgacagta gaagccccag agatattttt ttgctgtttg
    26341 atcaattcta atgcactgtt aaaacatgca ctgaaaccag ctgcaaacaa ttgttcaggg
    26401 ttggttgcat tttcgacgcg tttaccagga gctgcaatat tcaattcgaa tgtacgatcg
    26461 ggattatgaa cttctccaga gcgtccgcct gtgttgatca ttttcgtaga atacattttt
    26521 ttcataaaat taccaccttt aattatattt tatacaattg aattttacac aatataattg
    26581 tggaaagcaa agaaatggat tggcataaga taaataagag aaagcacatg aaacagacgc
    26641 gctttctcga atagcaaaca tatacaaaaa aatatcagag acaaaaacta atctcatcat
    26701 aaacattaca tatatttctc aaaaagttgt tttctttctt caaaacttac ttcacgatgg
    26761 acctgatgaa gcatccataa ataaccaaaa ggatctctga acattgagtt agtcacacca
    26821 aagtccatca tttctgtcat ttcttgaatc tctacacagc catttcgtaa tgctttatta
    26881 tgtgtgtcct cgatatcaga gacgatgata ttgaaccaga tcgtatttgg atgttcagca
    26941 gtaggagcag tcaaaccaag agacgtgttt tcatccagca tatggaatct cgtgccatac
    27001 aaggtaaaaa tgacttcatt attgcctcta gggtaatctg ttgcctcaat cagttgtatt
    27061 tcatcaaaga ttgtttgata cagttctagt gctttcaaac tatcatttaa tacaaaatct
    27121 atttccgttc ctttatgcat aaagatctcc tctccttttc agtcgaacag ttactagatt
    27181 cttttattat accagttaaa gaaaacgcat tcaaaaaaat agcggattta cttaccaact
    27241 cattcgattg gatgataagt aaatccgcgt cccattactt ctgctacgct gctgacaatg
    27301 acaaaggcac ttggatcgat ttcttctatc aatggtttca ttcgggaaaa ttcatgatca
    27361 gtcacaacga tcaataaaac ctcttttgac tttcgattat acccgccttt tgcctctagc
    27421 agggtaaggc cacgtccaat ctcagcactt aagcgaaggc ggatgtcttc caaataatgt
    27481 tcactcatga ccataacaga tttctttcga ttgatacctg tttcgatata agtcatgaca
    27541 attgaagtga ttgcaataga taaaatagca taaagaaact cttctattcc aaaaacaaac
    27601 agattaagag aaacaacgaa tgcgtctgta aaaaataagc caatcgaagt attcaagtgg
    27661 aaatattttt taaaaatgag gggagggatc gtcgtaccgc cgctagaaga gttgttttta
    27721 tataaaatcg caactcctaa agcaaaaatc gcacttccaa aaatgacaga caacaaccga
    27781 tctgaagtaa tcattgtttc tggaactatt gcaatgatta aaggaaaaaa caagcttccg
    27841 aaaagtactt taaaaaaggg ttttttacct aaaaatagaa gagctagtac caacataacg
    27901 atattcaaca ctaaaataac tatagctcgg tcaaatccta aaacagcttc cagtaatatt
    27961 cctagaccgc tgacacctcc agcagcaata tgatgaggcc ctaaaaacat attgatagcg
    28021 actcctaata tgaagagggc aaaaataatc ttaatgtatg tccatataaa ttgtctcgtt
    28081 tctttcattt atccacttcc ttaattcatt aataatactc ttatcttatt ataacttact
    28141 tttcacctta agttaacttt aattaataaa aaaattaggc tagtctaaat tattatgttt
    28201 cattcttcta gaatatactt ctacgttaaa aactgttaaa aaacttttac tttagaaaat
    28261 tggagaaatt gatcgcttaa gataaattca ggacactaaa tagtacagga agtagattaa
    28321 aacttttaaa agttttaagt ctatctattt ggcaagttta aaaaataaaa attcggtatc
    28381 aaaaaactgt taccgataac atttttgaaa gcgttgtctt ttgtttctct atcacttata
    28441 gtgatgacgt catattaaaa cacaaggagg aaatcaatgg atacagctgc aatttatcat
    28501 cgaccggaaa gtgaatatgc atttctatat acgaatacaa gatttcgtat tcggcttcgt
    28561 actaggaaaa atgatatcaa aaaagtatat gtgctttgtg gagatccata taccataaca
    28621 actgaaaaat ggtatcaaaa acaacgacca atgaaaaaat gtctaagtac caatgttcat
    28681 gattattggg aaattgaagt gacgagcgaa acaaggcgct tacagtatgc gttccatgtt
    28741 gttggggaag ataacacgga ctgtttttac ggtgatcaag gaatttttcc ttatcgagaa
    28801 aatgtgatta ctgaaccaaa tttttatttt cgaatacctt attttcatca aatcgatcga
    28861 ttcactattc cagaatgggt aaaagaaaca gtatggtatc aaatttttcc cgaaagattt
    28921 gcaaacgggg acaaagcaaa tgaccccgaa cacactttac cttggggaag taaagatcca
    28981 gaccgagaag acttcttcgg gggagatttg caaggtgtga gggatcattt ggattacttg
    29041 acggaccttg gcgtcaatgg catctatttt tgtcccatat ttaaagccac atccaatcat
    29101 aaatacgata cgattgatta ttatgaggtg gatcccgctt ttggcgataa aaaactttta
    29161 aaaaacttga ttgatgaagc acataaaaga gggatacgta tcatgttcga tgctgtattc
    29221 aatcatatgg gtgtccattc accacagtgg caagacgttc tcaaaaacgg agaaaaatcg
    29281 atctataaag actggttcca catccgtttt ttcccagtag attcttatca gatgactgac
    29341 ttaccagaaa ctgctgaaaa tatcccatat gatacttttg cttttactcc attcatgccg
    29401 aaactaaata cagcaaatcc agaagtgcaa aaatatttgc tggatatcgc aacttattgg
    29461 ataaaagaat tcgatattga tggatggcgt cttgatgttg ctaatgaagt cgatcatcat
    29521 ttttggaaaa aattcagaga agcagttaca gaaatcaaac cagacatcta tatccttgga
    29581 gagatttggc attcctctca agcttggctg caaggggatg aattccatgc agtgatgaat
    29641 tatgcattca cagattcgat taaagattat tttgcaaaaa agaaaatcac agcttcacaa
    29701 atggtcagtg gaatgaatca tcagcaaatg ctttacagag atcaggtgaa cgaaggaaca
    29761 ttcaacttat tagattctca tgatactgcc aggatcttga ccctttgcca aggtaacaaa
    29821 gaattgatga agtctgtgat ggcctttatg tttcttcaaa aagggtctcc ttgtatctat
    29881 tacgggacag aaatagggat gactggtgaa gatgacccgg attgccgcaa atgtatgata
    29941 tgggagaagg aggaacaaga tctagagcta ttcggtttta taaaagaact tgtttctcta
    30001 aggaaacagt tgtctaagat tatttctgaa ggttctactc agtggctaat agtatatgat
    30061 cgtgaagata agctctattt cacacgggaa ctagagggac agatcatcta tgtttacttt
    30121 aatcagtcaa aagaaccttg ggtagtagaa caggaaaatg aggtgattct tagtcaaaat
    30181 tgtcagttgt tagaagacgg gaaagcagag atacgacaat acggattttt gatcatgtgt
    30241 tgaaaactgt taccggtaac aacaagaaag cgtttgcaaa tgaagatttg tgcccattat
    30301 aataagggtg taacagcacg ttgaatacac aggaggaaat gaagatgaaa aaaagaagtg
    30361 tgaaacagtt gggtttaggg ttaatctctt taggaatctt aggcggatta gcggcttgtg
    30421 gcaatggtaa ctcttcagca gaaggggaag ccgatgaagg aaaaacgtta acagtttccg
    30481 ttgattctgg atataaagat tatataaacg aaataaaaaa cactttcgaa aaagaaaacg
    30541 atgtgaaaat caagctagta gaaaaggaca tgttcgatca gttggaatca ttagcgttag
    30601 atgggcctgc tggaaaggga cctgacgtta tgatggcagc atatgaccgg atcggtgcgt
    30661 taggacagca aggacatctg gcggaagtaa aactgggaaa tgaagcagct tacgatgaaa
    30721 cagacaaagc ccaagtgact tatgatggaa aaatctacgg agaacctgca gtaatcgaga
    30781 cccttgtttt gtactataac aaagatttag tcgatacagc accatcaacg tttaaagatt
    30841 tggagaatct atccaaagac agtcgttttg cttttgagtc agaagcagga aaaaatacag
    30901 gatttttagc taaatggact gacttttatt attcctatgg tttaattgca ggttacggtg
    30961 gctatgtatt tggtgatgat ggcacaaatc cttcagatat cggtttgaat aatgcaggtg
    31021 ctgtagaagg aatctcttat gcaacagatt ggttcaaaaa tgtttggcca aagggaatgc
    31081 aagacataaa aagtgcagga gacttcgcca gccagcaatt catgtccaat aaaactgcag
    31141 caatcattga cggtccatgg caagcgcaaa catacaaaga aaacaatatc aattatggtg
    31201 tagccaaaat tcctactcta aataatggac agccttatca accgtttggc ggaggaaaag
    31261 gatgggtagt cagcaattat gctaaaaaca aagacctctc tcaaaaatgg cttgattatg
    31321 taacgaatca ggaaaatcaa gaaaaattct ttgaaatggt aaatgaaatc cctgcaaacc
    31381 aacaagctag ggaaacagca aaggctaaaa acgatgaatt aacaacagct gtgattgaac
    31441 aatatgaaac agctcaagcg atgccaaaca tcccagaaat gggcgaagtt tggacaggtg
    31501 cagaaaactt gatgttcgat gcagcatcag gttcaaaaac accaaaagaa tccgcagatg
    31561 aagcagtaaa aacaatcagt gaagccatag aacaaaaata ctgattaaaa tgataaccgt
    31621 ccatttttat aaaaaatgga acaggtgctt cagataaaag tggcaagagc gagatggaaa
    31681 attatcttgc tcttgttgcc attatcttaa aagcagtttg cggtttagca agtttagagg
    31741 gggcattcag atgcagcaag ataaaacaag caatatcaac gaaaaaaaaa tcacgctttg
    31801 ttcacttatt ccagggatgg ggcagtttgt aaatggccaa gcttttaaag gtatcgtttt
    31861 tttcgccata ttcgtcctat tcattggtca gatgcttctg ggcggatttc atgcattggt
    31921 aggattggtt actttgggaa gtataccaat ggaagatcat tcattattta ttttgattga
    31981 aggcactttg caattcatca ttacaatcat tttcttgatt ttttacggtt tgaatctttt
    32041 agacgcttat cgagtagcga agatgcgtca agagggcaaa aaaatcaatc ggacgatgaa
    32101 ggaagtcata cataatatag gtgatgaagg ttttccttat ctattgacac ttcccgctta
    32161 tcttctcatg atttttacga tcattttccc agtcctagtt acattgttca ctgcatttac
    32221 taattatgac ttcaatcata ttccaccagc cagtttaatt gattgggtag gggctaaaaa
    32281 cttttttagt atcttctttt taagctcata tcgtaatact ttttttgcag tcttttcgtg
    32341 gaccgtgatt tggactttat gtgcaacaac tttgcaaatc acccttggta ttctcacagc
    32401 ggttgttgct caccagaaat ttattaaatt caaacgtatt ttcggtatca ttttcttatt
    32461 accatgggcg gtcccagctt ttattactat catgagtttt tcgaatatat tcaacgatag
    32521 cgtaggtgcc atcaatactc aagtgattcc tttcataaac cagcttcctt ttgtagatat
    32581 cagtgcaatc tcttggaaaa cggacccgaa ctggacgaaa gtagcaatta tcttagtgca
    32641 gggctggtta ggatttccct atatctatgt gatggttaca ggaatcttac aatcgatttc
    32701 agaagactta tatgaagcag caaaaatcga cggagcaaat gcatggcagc gcttttcaaa
    32761 catcacgctt cctgctgtct tcctagtggc agcgccaaca tttattacac agtatacggg
    32821 gaatttcaat aatttctcca tgatctatct gtttaacaat ggtggtcccg gaagcgtagg
    32881 cggaggagct ggttctactg atattcttat ttcctggatc tataaactta cgactggaac
    32941 ttctccgcag tattctatgg cttcagcaat cacgttgatt atctccgtca tcgttatctg
    33001 tgtgtcattg ctggtattta aaaagacaaa tgcgttcaat atgggggatt aacaatgaaa
    33061 aaatttcatt cgagtgctcg attccaaaaa cgactttctc acttttttac ttactttttt
    33121 cttttaatac tgtcagtcat tatcatctat ccgcttttga tcacagcaag tactgccttc
    33181 aagtcaggaa atattgcagc ttttagttta gatctttcaa gtgactggac tttaggaaac
    33241 tttactcggc tattcagtga aacactttac gggacttggt ataaaaatac attgatcatt
    33301 tctgtcctaa caatgatcgt acaagtgact gtagtaactt tggcgggcta tacttatagt
    33361 cgctatcgtt ttatcggccg caaatcaagt ttgatgttct ttttagtgat tcagatggta
    33421 ccgactatgg cagcattgac tgccttttat gtcatggctt tcttattaga agcgctcgat
    33481 aagtactggt tcttgacaat gatctatata ggcggtggaa ttccaatgaa tacttggctg
    33541 atgaaaggat actttgacac tgtaccaata gatttagatg aatctgctaa acttgatggg
    33601 gcagggcatt tcaggatttt tttccagatt gtattaccgc ttgtcaaacc gatgctcgcc
    33661 gttcaagcat tatgggcttt tatgggacct tttggggatt tcatgttagc taagttcctg
    33721 cttcgaacac ctgaaaatca gactgttgct gtcggcttac aaacatttat ttctgatgtg
    33781 acaaaccaaa gagtctctct ttttgctgct ggggcaattt tgattgcact accgatttgt
    33841 ctattattct tctttttaca aaagaacttt gttaccggcc tgacatctgg cggaacaaaa
    33901 ggataggagc agagtatcat gcaaacattt ccgttaaact atttttccgc attacgtacg
    33961 ccaactcaag tgtttgcggg acgcaaacta ttatcatggc ctaaattttt cttgatattt
    34021 gtatttttag tttccttgat ggtcatgcct gtgactcttt tttatgccaa tcagatccaa
    34081 gcgattccat tagaacaatt tctatccgtt cattcgctga tcgatgaaca aggaacccaa
    34141 aaattttctg aacttgaatt atctgaaact gggttacaag catcgcaaca aacgatagct
    34201 gtgacccctg aaattttggt aggtgtgtct ttatctgaga aacaacaatc cgatcatgga
    34261 acttttattg attttgagaa ggaacaatgg gtgatccaac aaaaggataa atctggtata
    34321 cgccgctata cgatgaatta tagcccatct ttccaaccag attctgtacg tacgcccgaa
    34381 gatttccaac gatttttaga aagagaattt tatgccagta atcgtccaac aatcatttta
    34441 tcttatagtt taagtcttgg tcttgtccta tttgtcatga cgagtctcat tttattcggg
    34501 gcttcctttt tcttgtggat gacaagaaaa agccaacttt catccattca tacattcaaa
    34561 gagagtgcaa atttgatgct gaatgttatg ggaataggtt ccatggcagc agcagttgta
    34621 gggtttatcc actttgactt tatcttgatg ctaagtgtcc aaacaacgat aactgtcttg
    34681 ctgcttttat gggtttttgt caaaacaaga ttcaaagata agcgtgtcga atgacaagtt
    34741 taagcgattt atgcgacaat gaatgtgaaa aaaatgaaaa ataaagtagt ttttgaaaaa
    34801 tttctgaaaa aattaaactt cttagttgat tcttttcttt tttagtatta acgtaatagg
    34861 gtagtttttt taaggaggaa aatcatgtta gagaaaactt tatacaatca attattttca
    34921 cgttctttct cgctccctgt cgaagtaaat tattgggacg gtacaacgaa acaatacgga
    34981 gatacggaca ctcctcccca aatcaaaatc aatatccatg aagaaatccc aatgaaagag
    35041 attacaagta acgcttcttt ggcactagga gaagcctata tggatcatcg aatcgagatc
    35101 gagggaagta tccaagcatt gatcaacgat gcttatcagc aagcaaacag ctttttacga
    35161 ggaaaagatt atctaaaatg gctgccaaag aaagaaaaac atacgaaaca aaaaaacaaa
    35221 gaagatatcc atgcgcatta tgacttaggt aacgactttt ataaactttg gctagatcca
    35281 actttgactt attcttgtgc ttatttcaat acacctgaag atacgttgga acaagcacaa
    35341 atcaataaag tccatcatat cttagacaaa ctctttatca aagaaggcga aacgttgctt
    35401 gatatcggtt gtggctgggg aacattgatg ttcactgcta ccaaagagta taatgtcaag
    35461 gcaactggga tcacactaag tgaagaacaa tacgatttga ttaccaaaag aataaaagaa
    35521 gaacatttag aagataaatg ccgtgtatta ttaatggatt accgcgaact aaaaggcgaa
    35581 acgtttgatc atatcacaag tgtaggtatg ttcgaacatg taggttctga aaatcttgaa
    35641 ggttatttca aagtggtaaa agatttattg aaaccaaaag gaacagcttt gatccatgga
    35701 atcagccgac aacaaggcgg tgcgacaaat gcttggatca ataaatatat tttccctggc
    35761 ggttatgtcc ctggtgttac tgaattagtt gataatatta caaaaaacga tttgcaactg
    35821 attgatttgg aaagcttacg tcgtgactac caattgacat tagaacattg gacaaagaat
    35881 tttcatcaag tccaagatca agtcacgaaa gaaatgggtg attccttcta tcgaatgtgg
    35941 gatctatatt tacaagcttg tgcagcttca tttgaatcaa gtaatatcga tgtgattcaa
    36001 tatttgcttg ttcaacctgg aaacaatgac atcccaatgc atcgtgcata attgaggcaa
    36061 aagtactgtt taaagactat ttaaaaagaa gctaagtttt atgatgctcc agaagtgaac
    36121 ttttttgtct aacttctagg attgtctcta aagcttagtt tctttttctt tatattttca
    36181 taataaaagt ttggttcttt aatttttaaa catgacagaa aattttttct tcgttatact
    36241 tgtaatagaa tactatttta taacaaaggg gatcgaatca atgaaaaaaa ctgttattta
    36301 tactagttta tttgcagctt tgtttttagc tgcttgtcaa tcaaatacca atcaaggcac
    36361 atctgggaat acgtcttcca gtagcgaaca gcgctcttca caggtatcgg actcctcaac
    36421 aaaagcaagc acatcttcgt ctagccagac aaaaagttct agtgaagcaa gtactcagtc
    36481 agatatcaaa gtgtctgcag ctcgtgcgat cgaattgttc caagataaat acccagatac
    36541 tgcaattact agtttggaat tagatagtga ttggggatct tacttctaca agatagaagg
    36601 tgtcgatgat caaaaagaat acgaagcaag aattgatgct caaaatgaaa aaatggagac
    36661 agagaatcca gaaactctag atcgagatga acaaaatggt caaaagaaaa aagaagatgg
    36721 tctggatgtt gataaattga tcacgattga ggaagcagca aaaatcgccg tcgacaaagt
    36781 aggaagcggt acggcaacag attgggattt ggataaagat ttaggtacta cttattggga
    36841 agtcaaagta aaagacggca atcaaagtac atcagtcaag atcaatagcc aaacaggcga
    36901 agtactgtca actgaacaag atgattaaga atttcttcgg aaaacaagag ttgatttttc
    36961 ttttagtcat gatacactag acaaaataac ttgtttgaag gagcgattta cttgagttta
    37021 gaaacgacct ttgccaatca tatctttgcc aatcctttga tgaatgcatc tggtgtccac
    37081 tgcatgacta cccaggaatt agatgaactg gctcattcag aagcaggagc atttatcact
    37141 aagagctgta cgatcaatga acgaaaagga aatcccgaac cacgctattt tgatgttcct
    37201 ttagggagta tcaattctat gggacttcca aacttaggtt tttcttatta tctagagtat
    37261 gctcttgcgt atgaaaaagt gcaggaaaac caaaatcaac cgttattttt ttccattgcc
    37321 gggatgagtg tccaggaaaa cttggaaatg cttgagaaaa tagaaaaaag tgggtttaat
    37381 gggatcactg aattaaattt atcttgtccc aatgtgcctg gaaaaccaca actagcttac
    37441 gattttgagg ccacttatga aacactgaaa gaagtattca gcatcttttc taagccgcta
    37501 ggaatcaaat tgcctcctta ttttgatttt gctcattttg atcagatggc tgatatcttg
    37561 aatcagtttc cattgaccta tgtcaatgct atcaacagtg tgggcaatgg tttgtatatc
    37621 gatacagaac aagaagcagt agtcatcaaa ccaaaagaag ggttcggcgg cattggcgga
    37681 gagtatatca agccgactgc tttggcgaat gttcgcgctt tttatacacg tttgaaacca
    37741 gaaatccaaa tcattggtac aggtgggatt cggacaggac aagatgcatt tgaacatctg
    37801 ctatgtggcg caagcatgtt acagattggt acagagcttc ataaagaagg gccggaaatt
    37861 ttttctagaa tcataaaaga attaacgcag attatgtcag aaaaaggata tacgtcaatt
    37921 gacgaattta aaggcaaatt acgcaccatt tcatagcaaa gagacgaagg tcatgattga
    37981 tcttcgtctt tttttataaa aaacactcgt ctgttcattc ctgtgcaagc gtactcattg
    38041 tatgttagac gaacttgtgg gaaaataaca gaggcaaaaa ggaggataat atgaatgatg
    38101 caacagaatt agcaaaagag cttaaagaaa aagaatttag ttttcaagaa ctagttacgg
    38161 atatacagaa aaaaataaag aagcaaaatc atgaattgaa tgcgtttgtc gcgcttgaac
    38221 cattcgagag taattcccac gagctattga ttccccagtc agaaaaaggg ccattggctg
    38281 gtatcccatt tcctttaaaa atgctcggtc aagaaaaaaa gggatggcta gctacttcag
    38341 gatctcgttt atttgaaaca caccgagctt ctcatacatc taattatgtg cggcaagcag
    38401 aagcgatagg tctagttcct tttggacaaa ccaatgcgcc agagtttggt ttcaaaaata
    38461 ttacagaccc agtcatctat ggcccagcaa gaaacccttg gaaccttgaa tgtacaccgg
    38521 gcggatcaag tggaggagct gctgctgcag tttcatcagg aatcgttcct ttagctggtg
    38581 ctagtgatgg cggtggttct attagaatac ccgcttcgtt ttgcggattg attgggctga
    38641 aaccttcgcg agggacgatg ccagttggtc catatgcttg gagaggatgg caaggagctg
    38701 ctattgattt cggcttgaca gtttctatga gagacacgga ggctttattt tatgggatga
    38761 gaagtatcca ttccggtgcg ccatatcaag tatcgccagt tgagtggcaa acacatccaa
    38821 gaaaaaaacg gttgaagata gctttttgtg ttgattcacc gatccactca cagattagtg
    38881 aagaggcagt acttgctgta aaaaaagctg tagatttcct tggaaaagaa gggcatgatt
    38941 tgacagagat tccttaccca ctagatggtc gtaaactgat cacgtcttat tatcagatga
    39001 atggcgcaga aacagcagca atgatccgct cgattgaaac aggcatccag cgagctgtca
    39061 gaaaagaaga acttgaaccc atttcttgga ctttgttcca atatggtcag aagctctctg
    39121 ccgctactta catcgattct ctgcatgtat gggatgaagc agcaattgtc atggaaaacc
    39181 tacatcaaga atacgatcta ttcctttcac caacgactgc atttaccgcc cctaaaatca
    39241 gcgaagattt gcaaagtgat gcgataagag cacagatgga acacatcagt gattgttctg
    39301 aagtagaagg tttagacgtt gtttcttcca tgtttgagga gagcttgaag atcacacctt
    39361 atacacagtt ggctaatctg actggacaac cagcaatcag cttgcctact catttgacaa
    39421 aagaaaacat gcctctagga atccaattta tggctgccag aggaagagaa gatttgctat
    39481 ttcaaatcgg ctatctattt gaagcaaatc atcagttcca tttaccaaat ccatggaaat
    39541 agcagaaaca aggagaagaa gaatgaagaa aaaaagattg tttgctctaa taactagtgc
    39601 cttttttatt ttaagcgctt gcggtacagc tgccgctaat caggaagata acagctggag
    39661 taatgtagaa aaaaagaaag aattagtagt agcgacttct ggcacactgt ttcccgcatc
    39721 ttattataat gatcaaaatg aattagttgg ttatgatgta gatttggcaa aagaggtggc
    39781 taaacggtta aatgtcaaaa tctcctttaa agaatacaac gtggatggac aaatcacttc
    39841 tgtttcaaaa agagaatcag attttgctgc gaacgatttt ggtatcacaa aagaaagaga
    39901 aaaaaaattc tccctttcag agactttcaa atattcattt gacagtatga tcgtacgaaa
    39961 aaaagatgat tcaggaatcc actcacttga agatcttaaa gggaaaaaag cagctggcga
    40021 acccaatact agctatatgc gtttagcgga aaaacacgga gctaaattag tcacatatga
    40081 caatgccacg aatgatcagt atttaagcga tgtagcaaat ggtcgaacag atgttattct
    40141 aaatgattat tatctgcaga aaatggccgt atctgcttta ccagatatcc ctgtaaaaat
    40201 actggagaac gtttatttca atgaaaacga aagtggtttt ttattgaaag aaggaaatga
    40261 tgctctgaaa aagaaaatcg atggcgtatt aaaagagatg aaagaagatg ggacgacaaa
    40321 gaaaatatct gaaaaatatt ttcaaactga tgtttcagtc aaaccgaaag aaacgatggc
    40381 tgagtcggtg agtgagtgat gtactttccc ctaattgatt tttccctcat gtggacttca
    40441 cttcctttcg tactgcaagg cttggtatac acattaggga ttggcttcgt gtcgtttctt
    40501 ttaggaaatt tggtagggtt gcttttgaca gttttaggtc ttttggattg gctgcctctg
    40561 aatgtattta ttcgttttta tttatcgttc tttagaggca ttcccgcact agtcctgctg
    40621 tttctgttat atttcgggtt accttatcaa ttaagtgcct taacggcttc agtcatctgc
    40681 tttacgatta ccagcagtgc atttattggt gaaatctatc gaggatcact agcaggcgtt
    40741 tcttcaggtc agtgggacgc tggtttagct tctggttttt ctttctttgg ggtattgaaa
    40801 tatatcgttt tgccgcaagc ttttcgaatc tcagtaccgg cactcagtaa cgtagctatg
    40861 gatctagtga aaggtacttc gttagcagca acaatcacag tgccagaaat atttcaaaaa
    40921 gcaaagatca taggcggtcg gacattcgat tatttttctc tctatatttt agttgcactg
    40981 atttattggg tcatttgtat cgggatcgaa aaagtgcaac agtatttaga aaaacgttct
    41041 atccatcagt caatctgaaa aatagaaatg gtgctagcgt ggctggcacc atttctattt
    41101 tttgtttctt gtatattctg cctagtattt taaaaccatt tcatgtaaaa tgtaaatcga
    41161 aaatatgttc ggaggtagaa aacatgaaga ccccgctaac catcgaaata gaatcctcgt
    41221 tataccaata ttgcatcgag caaggcggct tggttgtaga agaagtcacg atgcctgatg
    41281 accaaggaat tgtagatacg cttagctgct tgacaaaatt tgatggaagc cgagaatggc
    41341 gatgctatga gctgaaagtg acaaaagcag attttcgctc tacagccaaa ctgtcatttg
    41401 ttggacatta taattatttt gttttaccaa gtaaactata tgaagaagta aaaactgaaa
    41461 tccctacagg aataggtgtt ttagtgtatc gtccgtacac ggttgaagag gagatgcctg
    41521 cctctggtac atttctgatt gcgaaaaaag cagcaaagaa agagcttgct gtaccggaag
    41581 aagctttgac aaaccgcttc atggcttctt tattcaggga agtaagaaaa gcaaagcaaa
    41641 tggagcgagg cgtaaaatat tttccaaaag atcaattgta caaagaattg aaaagacgtt
    41701 cagaaaatca agatccattt tctaacgaac gatattatga acggttttta gaggaaatag
    41761 ctagtcaggc aatcaatgag ttgcaagaag aagttatcgc gcttcagcat gattatgaat
    41821 atttgaggca acaacagcag atccgtcggt tgcctacaga gccattggag tgaaaaatat
    41881 gtactatcct tatttaagag gaaaacaatt tgacctatta gcattaaaag aagcactgtc
    41941 gcgaggattg ctctccaata aaatccaacc agtgatcgaa ccagtacgtg attccgctac
    42001 gcttaaaaat gtgatcgaat tatttcaaaa aaagcaccat ccaatcgctg tgatccaaaa
    42061 tccccaagta ggacaattca aattattcga tcagcatgtg cacagttgga cagtcaaaga
    42121 aaatagctct gtcgtccctg cacagatctt aacgccagaa aatctcagtg aagtactgga
    42181 ttcacctcca gcgtttcttg ttttcgatgg acagcactac ccaaaagata cagaggtgtg
    42241 gaaacagttg gcaggagttg attcgaaatt tttgatacca gatactagtc gctttcgcat
    42301 ctggctgccc gaaaacaaaa ttgtgatccg tgactctttt caaacgagaa agcatgttga
    42361 aagttatgca gataaaacag atgacttttt tagtgatgac tatctttttt tccacgcaga
    42421 cggatatatt ggattttctg atttcacgat cgaaggtagc cgttatttcg ataaagggtt
    42481 ccctagccga gcgcttgcta tccatctaac gtatattgat gcttacggca atatacgcgt
    42541 gaaacatttt gtctctgaca gtaacgactc tgctaaagac caagcattga agtttcttga
    42601 agcaggagat aaaatgaaaa aatggatcat gcgtcaccat catcaactct tgattacttc
    42661 aggaatgatt gaattattgg ctctttacca gcaacaaaaa ttccctggac tgggagtgtt
    42721 gaaaaagtgg tcacttatgc atcatttgga acttattagt cacctgctag atcatccaaa
    42781 agattggctt aaatattatt caaaagttca ggaactatat gaagtgatcc aatgattaga
    42841 taatgtaaat gaaaaaggaa tagaaaagag gaaactggat gtatattcgg caaacaagga
    42901 aagaagaagt agaagaagtg gtagaaatca ttaatgccgc agcggatttc attgcttcgc
    42961 aaggctctcc tcaatggcaa aacgggcaga aaccaacaaa agaaacgatt ttgaacgata
    43021 ttgaaagaaa agaaagctat gtattaatag tggaagatca aattgctgga acagctgcat
    43081 tagtctcagg cgtggatccc gtttacacaa aaatagatgg tagttggaaa gaatcaactg
    43141 gtccttattt atcgattcat cgtgtcgctt tagcagaaag tactcgagga aaaaaactag
    43201 caaaaccgtt cttagaacat ttgttgacga ttgcagccag caaaaatatc caagatatcc
    43261 gtatcgatac ttatccagct aatgaaccga tggaaaagac catctattct gctggattca
    43321 cttatcaagg gatgattgaa tttccttttg aacacggcga aagaaaagca tatcaaataa
    43381 tcatcaattg actataaaat catctgagca aagataaaga cattaacact actgaaaaaa
    43441 acaatcatat gatagccaag aggttgtaac agaagtggcc agcttcaaaa aacaagaaag
    43501 aatttccgaa aatgcttctc aaatttttgt ggaattttag cgtattttac gaaatagctg
    43561 ctttctgaaa ctcccttcat cgagaggtcg tgacatagct tttgtcacga tctctcggct
    43621 tatcctacga gtattattcg atacatttat atcaataatc ctttttttat tttatctgca
    43681 gcttcttgga acgctccatc gtttgacacg actaaaagtg aggggatagc tttcaaacgt
    43741 ttcaactgtg ctaaatcttc tacataaacc gccagtctgg ctgaaatctg ttcagatgaa
    43801 tctccacgtt ttttcaggcg cgattgcaat gtttctttag agatcgtaat aaagatagga
    43861 cggactaccg tcccgaaata ctcatataaa tgccaaaaac catttgctgt caaaacggcg
    43921 taacaatttc ctactgtcag tttcttttta atttctgctt tactggaacc atatttatgt
    43981 tgagcatatt ggtcccactc tgcaaactca tttcccttga tcatctgatt gaatgtattg
    44041 tcagaaacaa aataataatc ctcttgatct ttttccccag gtcttggtga tcgattcgtg
    44101 taagtaatga tcttttgttc cggctgaaaa atagcttctg cgagtgaagt ttttccagaa
    44161 ccagaaggac cgataaatac atagcaataa ttcattgtca tttctccttt gatgattgaa
    44221 caagtgaaaa agctttggaa aatatttcca aagcttttaa aagtaacttt gcttttttgt
    44281 attttatacc agcaagccag tcacaatagc acttaaaaag ctgactaaag tcgctgttag
    44341 caaaattttc aatccgtgtt ttgcgacaag atttcctttt tcttcattta atcccttcat
    44401 cgcaccagaa atgatcccga tagaagagaa gttggcaaat gaaacgagga agacagatat
    44461 aattgctgtt gtacgctcag taaaatggaa attaccattt gctaattctg tcatagcaac
    44521 aaattcattt gtaaccagtt tagtcgccat gatactaccg gcatccactg cttctttcca
    44581 tgggattccg ctgatgaagg caagaggggc aaagatgtaa cctaggatct cttggaaagt
    44641 gatgccgaat atcccgtgga agatagcatt gatcatggca atcaaagcaa caaaaccgat
    44701 aagcatagca gcaaccgtga tcgccacatg gaaaccatca agaatgtatt ctcccaacat
    44761 ttggaaaaat gtctgttctt tgttttcttc aatgatcagc tcatcttctt tttcttctaa
    44821 tacataggga tttacaattg aagccagaat ataaccgcca aataagttca aaacaagggc
    44881 agtgatcaca tatttagaat cgatcagtgc catataagat ccgacgatcg acatagaaac
    44941 tgtagacata gccgatacac tcatggtgaa caaacgtttc tcagatagga agggtaattc
    45001 cttttttatg gaaataaaaa cttccgattg tcctaaaata gcagaagcta ctccattata
    45061 agattccaat ttgcccatcc cgttgatttt acttaatcct agaccaacag ctttcataaa
    45121 taatggaaga atcttgatat agcgtagaat accgataata gcagaaataa atacaattgg
    45181 catcaatacg gaaagaaaga aaggtgcact tcctttattt gctacaccgg caaaaacaaa
    45241 gtcaacacct tgcccagcaa aagcaagaag atgatcaaac atttttgcca atacagaaac
    45301 aaccgctgta ccaaaagttg tacgtaataa tacgaaacca agaacaagct gtaaaacgaa
    45361 catcacaatt agtggtttca atttgattgc ttttttgtta ctgctgatca aaaaagcaag
    45421 ccctaatgtg acaaataaac caataatacc taataaataa ctcaaataat cactcctatt
    45481 ccaaattccg ttttatttta cctgttttgc aaataaaata ctaatcttta gtgaaatttt
    45541 cattactttt ttagagtgaa acgcattcat tttccattag aaaataaagc gaacaaaata
    45601 aaaggtttaa aaagtgtaac gtcttagtaa agtctataaa aaaacgaaac tatcgattaa
    45661 gctaatagtt tcgttttcaa catttataag taaacttttt ctattggtaa tatagatttt
    45721 cggaagggta gacaggtgcg caggtaatat cgattcctag ttttttcaat gtttgttcat
    45781 catttttatt gaccatcaca gtagagtggg cttgtgctcc ctcaagttca gctagtttat
    45841 cataagcaac ttgagcgatt ggattcgtta ctgcactgat tgataaagcg atcaagactt
    45901 cattgatgct tagtgcatcc aaagaggaat gaagtgtctt tgatttcaat ccttgtattg
    45961 tttcaagaac taatggtgac aataaaggaa tgtcatctgc gatatgggct aaatgtttta
    46021 ctgcatttaa gataactgcc gctgacgcat ccatcaatga acttgtacgc ccggtaatga
    46081 tgacttcatc tggtaattcg attgcaatca cagcagaagg atcttttgta ggactttgcg
    46141 cttgaatctt atctgcatag tcatgggcag ggactaccac gtttcggtct tcaggacgta
    46201 aaccgacttc ttccataatc agtttgattc gattaactgc ttcttcgtcg atcaatcctt
    46261 ttttgaagtc gcattctgtt tggaaataac gacggatgat ttcttgttta gaggcttctt
    46321 ggacaacggt atcatccacg atcccaaatc ctacacgatt cacacccatg tcagtaggag
    46381 attgataaac agattcttta ccagtaattt tttcaataat acgtttgatt acaggaaatg
    46441 tctcaacatc ccgattatag ttcacagcta ctttattgta ggcatcaaag tggaaggaat
    46501 cgatcatatt gacatctttt agatcgactg tggctgcttc ataagcaatg ttcagcggat
    46561 gtttcaacgg aacattccat actgggaaag tttcgaactt ggagtaacca gctgcatgtc
    46621 ctttttggct ttcatgatac agttggttca agcaagtagc gagctttcca cttcctgggc
    46681 caggagctgt cactacaacg attggtttag ttgtttcaat atactcattt tttccaaaac
    46741 catcttctga gacgattttt tctacgttga ctggatagtc ttcgatttct gagtgtttat
    46801 agaccttgat gtttcgtctt tctagtttat tgataaagac tttcgtggca ggctgtccac
    46861 tatagcgagt gataacgaca ctgttgatta ataagccata accacgtaat tcatcgatta
    46921 accgcaagat atccatatca taagtaatcc cataatcacc acggatctta ttacgttcaa
    46981 tatctccagc atagacgcaa atcagtattt cagcttgatc tttcaatttt tgcagcaatt
    47041 tgattttcgc atcttcttca aatcctggaa gtacacgttt cgcatgcttg tcgtcaacta
    47101 acttcccacc aaattctaaa tataatttgt catagtggtt cactcgttcc aaaatatacg
    47161 ctgactgctc ttcaatatat ttttctgaat caaaaccaat ttttttcact aggttccctc
    47221 cttaagtact aaagcatttt ttcatttttt tgaggattcg tcaactggaa gagcgaaatt
    47281 cctcaaaaaa acaaagaaaa tggtttaaac caacttgtct agatagttat tcattggtat
    47341 gatagttttt gataaggaag gtgctacatg aaaaaaagac agaaaaagaa aaacgcatat
    47401 aaacactata tccgttcgat ttttaccggt tatgaaagaa tgcttgaaga tcccgaattg
    47461 gaacaattga cgtttactta tctaaacgaa gaaacgcagc taactcgtga tgagcatcag
    47521 cgcattcatt tcacaacaag agatctgcct agtaaataaa aaataagata aaggtcatcg
    47581 gattcgattc tgtcctatca gatggaaaga ctagttgatc gaaaaaaagg ctgtaccaaa
    47641 ttttggtgca gcctgtttta tggagcattg cccgctcttt tttataacaa ttttttacag
    47701 aaaatggtac tattgaagta ttaataagtg aaaaacagat gagtaagagg aggcaagtag
    47761 tatgaaagag ccagatacaa atgcaaatta tgagccagac gaactagagg agacgaacgc
    47821 ggatcaggat acaggacaag aatcgtcttc tttttacgat atcaaagaaa aagcaagcat
    47881 gaatctttcc caattattag aagacttaca ggcttttgaa aaagctgtcc gcgaagaaga
    47941 tatccctgag atctaccgtt tatacaatgg ccgattgaat gaagaattaa aaagaagctc
    48001 aaatgaaaat cacgagatcg atcaattgct gatgcgtaag atccatgatc gttttttaca
    48061 ggaatttcct tttatggaac aagtagaaaa catctcgcca acgatgagct attacaaaat
    48121 cggcacttat taccatgatc ggcccacggt cggtattgat gcaagtctgc cagaaatttt
    48181 tgttttgcct aagatcgatg aagagtggga gaaatattcc caaccggagt caacggacta
    48241 tgacaaaaaa atcaatgagc tagacgcaaa ggtgatcact gctcagacag aaattgaacg
    48301 tttagatgaa caaataaaag aaatcaatcg acaaatgact gatttgaacg acaataaagg
    48361 attcttgaat cgtaaaaaaa tcgaggaaga aattcaagaa ttagagaaga agaagcaagt
    48421 actatctaat gaaaaattag gctggcttcc ttatattgag acgcctgaaa caatccagca
    48481 gcaaaaagaa gcattgaagc aagaagcaag agcagaccag cttcgtgcag ccatcgtaga
    48541 aaaagaacaa aggcaaatca gacgctattt cggtagcaaa gagggattcg gtcaagcaat
    48601 ccatgaattc ttgatgaatt atcttggcaa tgagaatcct aataatcaat ctaatgaagg
    48661 aggttccgag tatgaatgaa cacgatgaag cagaatggac aaaaaatgaa gaacaaacag
    48721 aagatcatga agaaacgaac acttcattgg aagaacaaac aagtaatcct ctggatgaca
    48781 cagaagaaat ccctatgcta gaggaaacaa aaatacatga aaaagaaaaa gtaactccga
    48841 cgaatacgaa tactgcagaa aacacccatg cttacgaaat aactgaaaat cccgggtatt
    48901 tagaaggaaa agagcaagat catcaagata tcgtcagttt gattgaaaca gcggaagcag
    48961 aattagcgga tttacgtatg agaaaagatt tgatcgaatc agattttcca caattattag
    49021 aatcatatga tcgttttata ttgaatgaag aaccaatctc cacgatcgga aagacaagcc
    49081 tgatcgaata cttcacgtat gaattactga aaccgttgaa tgatattggt ctggaacggt
    49141 caaattcagc ttatgacaca tggaaaacaa gacatttttc gatcaattat acgttgaatg
    49201 gacaaaacga attagtcttt tcctttgtat taccgaccat cgatcaacga taccaagtgg
    49261 aatcgatgag tctcctaaac gtatcaccag agacaatgga aatcaatgtc caagatgacc
    49321 gggtgctttc tttgatccga cactggtcag tggatcgagt attctctgca gggcagatca
    49381 cgatattcaa tcacaaattg aatcaattat tagcccatgc aagaacactt ggttttaccg
    49441 ttaatcagac attactagac aatacgaagc cacttcgttt gaatcttcag agcgaatttg
    49501 aattgacgaa tgaagtattg gatgatattt ttatcgtggc aatgaaaaat ccatcctacg
    49561 atatggaaaa gatcaacgaa actacttatc aagtacgatt agataaagga cagtctttaa
    49621 cgataacaac aaacgaaaaa gatcaaacag tcttgtcgat ctcatctgga gactacccac
    49681 gatctgtcat tgatttcttt atcaattacg aattcttggt cccattaatg gtgagaaagt
    49741 cctgattgga taagttggat taccagatgt cattcagata attctgttcc aaaggacttg
    49801 cggcagttat ccagaataat tgtcacaagt cttttctgta tgtactaaat ggacgtgaat
    49861 aacttgctga tgaaggaggg atttcattag gaatgtccac aaataaaata gcacagctta
    49921 gcttattgtc tgctgcttgt gtagcaggta ggatcgcttt tcaatttatt ccgaatttcc
    49981 agccaatgac tgctattttt ttatttatag tactttactt aagcttaaaa gatgcgttaa
    50041 ttgtcatgag tctttctatt gcgatcagca gtttttattt tggtgttgga ccatgggtca
    50101 tcggccaatg gatcagctat actgttgttc tgagtttgtt ttcaatcgtc tgcttgagaa
    50161 aaacagtgca aaggaatcta tggttaaagg gagtctgttt ttttctggcg ggcatggttt
    50221 acggaatagg tatgaccgtt tttgatacgc tgttatatcg tttgcctcaa ccttggatct
    50281 attaccttca aggggtgtcg tttgatttga tgcacagtat aggcaatgtt gtgttctttc
    50341 ttgtttttct gccagtcgtc aaacgttttt acaatcattc aggaggaaaa aatgaaaaaa
    50401 ataatttata gtttgcttgc aatgagtgtc agtatactca ttttgacaag ttgcagtaca
    50461 acctatacac aatcggcttc caaccaaaag gaggaagtaa aaacatcgca aaaagcaaca
    50521 atcacgttac aagaaaatgg tcaaaacttt tcggaaaaag aagtggtctt caaaaaaggc
    50581 gatgatttac tgagtatttt gaaacgaaat tttgaagtca aagaagataa cggcttcatc
    50641 acttcgcttg aagggcactc acaggatgag aaaaatcaac tctattggat gtttactgtg
    50701 gacgggaaaa gtgctactac tggtgccaaa gagatcaaat tgaatgatgg acaatcggtc
    50761 attttcaatt tgtctaaact ataagtttca taaaaaactg ttaaagggcg aatattgcca
    50821 taattcagtg agttcagttg atgaaagaag gaaaataaca tggaaaattt taaaaaaagc
    50881 ggaatgtttt ccgtaattgc tgctctaggg gctaatattc tagttgctat cagcaaattt
    50941 gtcggctatg caatcagtgg cagtgccgcc atgctaaacg aaagcatcca cagtatcgtt
    51001 gattgcagca atcagatttt gctgttattc ggtgataaac aagccaataa aggacagagc
    51061 gagttgcatc aatttggtga aggacgagca aaatactttt tcagtacaat cgtcgcaatg
    51121 atgcttttct tcggcggtgg ggcacttggg gtgatggaag cttttgaaaa gttgacgcat
    51181 ccatcccatg aagtgggtaa tccgatcatt gttatagcaa tactgttatt tggtttggta
    51241 atcgaaggaa gttcgttacg cgtggcaatg aaagaaatca aagaattgaa tactgaaaaa
    51301 ctttccacga tgcgcttttt gcgcgaaagt cgacacagtg aaatactgat tatttttacg
    51361 gaagacttgt gtgctgtaat cggtttggtc ttggctttag caggaacaat attgacttat
    51421 gcaaccggga ttgctgcatt tgatgcgatc agcggattgc tgatcggttt cttgctgatg
    51481 ggagccgcca ttttcctagc aaaagaattt tatagtctga tcatcggtga aagcgtgaca
    51541 gcaagcgatt tatcaaaaat caagttagct tttgaacgtc ctgagatcag tcggctgatc
    51601 gatataaaaa cagttcattt aagtccaacg gagattttag ttgcagcaaa agcagatatc
    51661 gttggtaaca aagagaatga agcttactcg gtcatcaatg atattgaaaa aaatatgcgt
    51721 cagtctcttc ctgacaagaa gatgtatata tacattgaaa cagataaata tgacccaaat
    51781 tattctcgta aataaaacgt ttattcatga attgaagcaa aaatgagaaa aatataatct
    51841 tgtttttaaa taaaacggct acaatttagc gaatttatgt tacaatgagt ccgctgttta
    51901 tttgttgtaa ttaaaggaga tattttaatg aacaaaaaga aaagtgcgcc atggcttcaa
    51961 gctttgcatg cagtgatgcc actttgtata agctatatac cagtaggatt agcttgcgga
    52021 gtattgttgc aaaaagttgg atttgatcct ctcttatcag gactgttatc gattctggtc
    52081 ttttccggtg gcgcgcagtt tttagttgca tctatgctta cgacgcaagc atcatttgca
    52141 acgacattat tgatggtttt ctttttagaa ttacgctata cattattagg agcaagtctt
    52201 gccggtttta tgaaaaaaga aaagcggacg tttttggctg tcttttctca atcactgaat
    52261 gatgaaaact atgcggtgaa ctacttaaag ttttcaactg acccttcctg gaataaaaga
    52321 aaggctttat atgtcaattg gttctcaatg actgcatggg ccggtagcaa tatgatcggt
    52381 aattttttcg gctcggtgat tcgagtagat gctgaccttg tccattttgc tttgacggcc
    52441 atgttcattt tcatgtttat catgcagatg aaaagcgcac tactgatctt tacaggttta
    52501 ttctcaggcg tattaggggt cgtattcatg gtattattcc aaaatacttt cggattgatt
    52561 gcagcaacag tcattgcttc atttgccgga tttatgctgg agaaggcatt caaaaaagaa
    52621 aaagctaaga aactaagaga ccgcggtaaa gatgtcaatg agccgctctt caacttcccg
    52681 gaacaggagg ccaatcacca tgactaaact atatttgcta cttttagttg cttgtctgtt
    52741 catcgtggca tatatcccgc gcttgttccc gatgctttat tttactcatc gaaaagtgcc
    52801 atcctggttc agtgactgga tgaaatatgt accggtggca ttatttgcgg cgttagcttt
    52861 taaagatgtt ttcattactc atgaacatct ggatattgct tggaatatca aaatagctgc
    52921 aatgattcta gtagcaggcg tggcttacaa aacacgttcg atggcgttgt cagttttgac
    52981 aggacttgcg tctgtctttt tgctgtctat gctttaaact gttttatttt attaaaaaca
    53041 gagattgcga cataagttgt tcaaactgat ctatccgaac tgcatttgca acagtcaatt
    53101 ctggatgcaa gtttattcgt agggataaaa aagattagtg ttggcttatg atgcaagctc
    53161 ttttttattt taggaattag agagggattt gtccttctat gattttttgc tataataggg
    53221 aagatgttta ttttatgaaa gaatagttga gggaatttaa atgaataaaa aattgatgca
    53281 aggaacattt tggctaacct ttgctaatct tctatgtaaa gttttagggg tagtctattt
    53341 gattccctgg ctaagtatga tggggaacaa tcaagatggt atgcttgcta cgacattgta
    53401 taatgttggc tatcttcctt atggattgtt cctgatgctt ggtacagtcg gatttccaaa
    53461 tgcgattgca aaaaaagtag ctgtagccac aaaagaagga gataacgctg cttgtcggct
    53521 gatttttaga agtacgatca atatcatgtt tgtcatcggt atcgcctctg ctgcactgat
    53581 gtatttattt gctcctttac tagccggaat cagtccgatt gctaacgtta ataatgggat
    53641 actagctatc agaagtttat gcccatcttt gattgctatc ccaattctta gtgcaatgag
    53701 aggatatttc caaggaaaaa atgatctgcg gccttatgga acttctttga ttatcgaaca
    53761 ggctgtgcga gtgatcgtta tcttggctgg tacttattac ttgcgcgtat tgactgacgg
    53821 aaccatatta gaagctgtat tgatcagtac agtcgcttct ttcttcggtg ggttagctgc
    53881 gattatccat atgtatatcg tcggacgcag aaaagacttc ttcgaactta aagatttttt
    53941 gatttccagc cgttatttcg aaagagaaaa ccgatctgcc tctgtctcta tcattcggga
    54001 aacactaccg tttatttttg tcggctcagt cattactatc gttcaaatga tcgaccaagt
    54061 aacgatgaaa ccactgcttc actttttccg tccagagatc gcagatcagc aactggaatt
    54121 cttattcagc cgtgcttctg tcaatccaaa taaattgaca ttgatcctga tctccatggt
    54181 aggaacagta gctataagca gcttgccaat attaagcacg atgaagaaaa aagatcggat
    54241 acaaatcgaa aaaacagtag gagatagttt ttcgattgcg ttattgattc ttcttccttc
    54301 attgactgga atgtctttat tagctggacc actatacaca ttgttttttg gctatgatcc
    54361 tgaaagtgtc ggatatttcc aaatggcttt gctagcttct ttgtttttct ctttattcac
    54421 gatcctatct acgatgcttc aatctttgaa tcaccatcgt tttgcgatcc gattaactgt
    54481 agaagcaatt atcctgaaag tcatcttcca agtaatcgga ttaggattag ttggcggata
    54541 cgggatgagc ttatcaaatg gcttagcatt cggtattgtc tttgttcgcg gctatcactt
    54601 tatgtgcaaa gaataccgaa tagcaccttt agcaaagatc agcaattttt tcttaaaaac
    54661 tttccgctcc accctgatta tgcttgcagt atgttttgcc gttttctttt tgctgaatca
    54721 gcgattgtca atggaatcaa aaagctatgc aatgatttat tgtgtggctg taggaagtat
    54781 tggcggctta gtatttttat tttctcaatt cggaaaaaat gggtttcgaa tgttgaaaaa
    54841 tgttaaacat catagcagaa gcaaagaata ggttaactaa tcgttttttg ctgtcacttg
    54901 aaaaacggca actcgtatga ttacgcttgt cgtttttcta ttttccaatt ttagtttcat
    54961 atctaatctt acttatgata tagttataag gtctggaaaa attctttgaa acgaggaaat
    55021 taaacacatg aaacttgcag tagttacaga tagttcggca tatctgccgg aacgtatcaa
    55081 gaaccatccc gacttattcg ttattccaat ccctttgatt atggatggga aaatttataa
    55141 cgaaggaatc gatatcgaag cggatgaata ttatggactt ttaaaaaaca gtaaagattt
    55201 tcctactact tcacaacctg tcgtagggga agttttgggt ttatatgagg aattgaaaga
    55261 gaaagggtat gacacgattt taagtatcca tctttcttca gggatttctg gctttgtcaa
    55321 cacattattt gcgatgaaag atgacatcag cggagtaacg gtcattcctt atgactctaa
    55381 gatcactagt atgccgatgg gacacatggt agaagctgcc ttagacttaa atgatgaagg
    55441 tcagtccctt gatgaaatac ttgcacatat cgatcgaatc cgagataata cctatgctta
    55501 tctgattgta gatgatttga acaatttagt acgaggagga cgtttgacaa atggtgcagc
    55561 gctgattggc agcttattaa aaatcaaacc gatcctgaca ttttctgaag ggaaaattgt
    55621 tttattcgaa aaaatccgtt caagtaaaaa agcatttgct cgagctgaag aaattattgg
    55681 tagaagaaat aacgaaatac aaccgccagt caaactttac gtgatacatg caaataattt
    55741 agaagtagcg attgcggaga aagagaaatt gcagaaaaaa tatccaaatg cagaaatcga
    55801 aattggtcat ttcggaccag tcattggtac acatttaggt gaaaaagcca tcggcattgc
    55861 catttcagcg caataattcc ttttattcaa aaaacatacc aaaaata
//
LOCUS       DACEEZ010000010        54155 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20313_2017
            SAMN12639966-rid9879223.denovo.010, whole genome shotgun sequence.
ACCESSION   DACEEZ010000010 DACEEZ010000000
VERSION     DACEEZ010000010.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639966
            Sequence Read Archive: SRR10023158
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 54155)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 54155)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741683.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741683.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:44:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,016
            CDSs (total)                      :: 2,962
            Genes (coding)                    :: 2,889
            CDSs (with protein)               :: 2,889
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 36 of 73
            Pseudo Genes (incomplete)         :: 29 of 73
            Pseudo Genes (internal stop)      :: 18 of 73
            Pseudo Genes (multiple problems)  :: 8 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..54155
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639966-rid9879223.denovo.010"
                     /isolate="E20313_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            <1..1806
                     /locus_tag="IWO89_000661"
     rRNA            <1..1806
                     /locus_tag="IWO89_000661"
                     /product="23S ribosomal RNA"
     gene            1900..2015
                     /gene="rrf"
                     /locus_tag="IWO89_000662"
     rRNA            1900..2015
                     /gene="rrf"
                     /locus_tag="IWO89_000662"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
     gene            2346..3191
                     /locus_tag="IWO89_000663"
     CDS             2346..3191
                     /locus_tag="IWO89_000663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HAQ0455439.1"
                     /translation="MDEMKLIGIDLDGTLLNSDQSISQKNARTMKHLPEDCFPFICSG
                     REVEDINNILKKSGLRIPAVGLNGSVGFDGEKKIFESSFDKNDVKKIDSVLSKFPTKI
                     YTNLGSYESQNYKNKMKKIFHEVGSEFSIKELNYELEYEKSICSIPYNNIEEIIEKDA
                     ISIYKFFIFIPNRQLKANIFNHLKKMLNVSVTESAPVNIEIVPQNVSKGVVFDRLESI
                     YNLKKPLRIAIGDSLNDLSMFESADISFAMGNSHQEIMKKADYVTVSNDKDGVSQAFE
                     RILTI"
     gene            3472..4692
                     /locus_tag="IWO89_000664"
     CDS             3472..4692
                     /locus_tag="IWO89_000664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair exonuclease"
                     /protein_id="HAQ0455440.1"
                     /translation="MVKFIHAADLHMDRSFEGLTTLDKTVQEKLLKTNLTVLSNIIEQ
                     AIINNVDFVLLAGDNFHQNRPSLKIQKHFSEQMKRLEKNHIPVFIIFGNHDFYQKERY
                     WFSFPKNVHLFTSETVETKKITIKSGETVSLSGFSYRQPWIQKDKVMEFPSRELTDYH
                     IGLYHGEPGVSQKGNYAPFQPSKMQEKGYDYWALGHIHVPTVLNEKQTIVYPGAPQGH
                     TKKEQASTSILLVELSKGSCQIHPIKVAEVYWKTKEISLRKARTTKEVIVHIRQQLSK
                     VEDGFSLIEIKLKDYDHLNTDVLERIQSGELLDYLLEEFSDRINDFFIWRISLIENTF
                     YTKTPLAASAKLMEQLFQYYQTPQDFQQILNEVYSHQEAARILSELPEYQEETLEKAK
                     QLLNQDFLFEEDQE"
     gene            4689..7409
                     /locus_tag="IWO89_000665"
     CDS             4689..7409
                     /locus_tag="IWO89_000665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HAQ0455441.1"
                     /translation="MKILRIEIAAFGKWRQKSFDFYSGNQLIYGGNEAGKSTIYQFIQ
                     AILFGFPSKGRKKKDYTPKDGSAYGGKIWLKHPVYGEFAVERYKQQNRGKSKVWLGDQ
                     VGSDELLEKLIAPLTKELFQQVFTFQQEQLMELDHLREKELHDALISLGITGSSQVFQ
                     KRQEYYQKAQQLFKKKGQKLLINQKLNEWKELQEKIHQKQEQEAYFGKLIQQEQEYES
                     QQQQLLAQLKEANKQFSLARQQELNFSLYEEWQSLKKNSTTGLPDEQLLLDLESFSKR
                     YQQTNERLEELEATLEKHSGMDQQSARYYFYLEQEQELKDLQQLQFTAEQLNEEWQRL
                     QIETRETNSELKILEQRWHWQKNPLPEEFLYEEEWKHLLAEERRIADLESQISVRLQI
                     AKEQQEMIEAEVNDLEETYPALLQNQSEIAPVSSSKPYMLMAGGAIIIAGIFVPMPLK
                     VILLLIGAGMIGYFFYKRQPKIKTKNDFSEIKGMWQEKLGQLDLALAQLAEAEEKQKR
                     TGEEKQILQQEVYRFADHRHLGKMDTLKLMKEYGQEVIDYQKLYGVFERKHKREQEIQ
                     EKLRQIDQKFEFVKEWLPIQDKTISEKMVLLSRFIQEMEQMKFARSYQQNTLLKQEIN
                     QLKKKQKVWIQEYSEQLTQAGLSYPSEVPVFLQKAKQAEQQMKRYNELSEMLTPLFPE
                     ELTKEKLSVRLRQLKEKQSQLQQEEKEASENRQRLQLQVEELQRDGTLDELYQQASRQ
                     KAKLQELLEEWGALEIAGTFLGDLSTEMSERQLPQLLRAAGDYLGILTKGNYQKILLK
                     NDTLTLTDGYTEFPIYELSTGTKDQLIMAMRFAYLSLEANRSICPIIIDDGWLHYDHQ
                     RKYQLAELLAKFGEKYQIICFSSDKEMVSYYHEMNQIVKQLEGANNEKNS"
     gene            7393..8337
                     /locus_tag="IWO89_000666"
     CDS             7393..8337
                     /locus_tag="IWO89_000666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HAQ0455442.1"
                     /translation="MKKIRDLVIDEQFEQFVLIKSADVRIAKNGKKFIAFTFQDTSGT
                     IDGKYWDASEEEINKFTAGTVVFLNGKREVYQGNPQVKILHMRLTRVDEPGEPSLYME
                     RAPLKKEEMEEEINQLLFEITNAHWNRIVRYLLNKYQKQFFEFPAAKRNHHAFASGLA
                     YHTLTMLHLGKSIADEYPELNRALLYAGIILHDLGKVIELSGPMSTEYTLAGNLIGHL
                     VLIDEEITKACLALKISEADEDVLVLRHMVLAHHGLLEYGSPVRPRIMEAEILHQIDN
                     IDASMQMMLTSIRQTEPGKYTDRIFGMDNRSFYVPKDL"
     gene            complement(8406..9416)
                     /locus_tag="IWO89_000667"
     CDS             complement(8406..9416)
                     /locus_tag="IWO89_000667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HAQ0455443.1"
                     /translation="MKKKSIILAATSALAVLTLAACSGDTNKDIATMKGGTITVSDFY
                     DEAKLESSNQSLVQRMIIYKVFNNKYGDKVTDKQVDAEYDKQAKSLGDTFESQLEAAG
                     YTKDTYKEYIRNNLAFEAGLKAHVDITDDDLKTAWKSFHPEVEAQIIKLSSEDEAKDV
                     KKSADDGDDFSKLAKDKSTDTETKEDGGKVKFDSTTTTIPAEVKEAAFKLKDGEISDV
                     ITTTNPTSYATEYYVVKMVKNQNKGNDMDKYKDQLKDIATETKLSDNAFTTKVIGEEL
                     KDANVKIKDDAFENVLSAFTTTSSSTKDSSETTASTKSSDTKSTDSTKESSTEETTDS
                     SK"
     gene            complement(9596..9940)
                     /locus_tag="IWO89_000668"
     CDS             complement(9596..9940)
                     /locus_tag="IWO89_000668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HAQ0455444.1"
                     /translation="MIKNFFKGLLFGAAAGTVGGLLLAPRSGNETRKKLIEELEEATT
                     LTNDLNNSLNHFKSALIETKETAESVIPAFQEAIKKDIEDFKFQAEPRITQINEQVEK
                     LKADLPEYPEQI"
     gene            complement(9933..10367)
                     /locus_tag="IWO89_000669"
     CDS             complement(9933..10367)
                     /locus_tag="IWO89_000669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HIT family protein"
                     /protein_id="HAQ0455445.1"
                     /translation="MENCIFCKIINQEIPSYKIYEDDKVYAFLDISQATKGHTLVVPK
                     RHVADIFEYDPELAGEVFSRVPKIAQALEKAFPEMKGLNILNNNRELAYQSVFHSHIH
                     LVPRYSKEDDFSIHFVNHQDSYGSEELKAIQETIVKQVSCDD"
     gene            10505..11242
                     /locus_tag="IWO89_000670"
     CDS             10505..11242
                     /locus_tag="IWO89_000670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ0455446.1"
                     /translation="MSLIIDHVTGGYGHLPVLKDISFDVQPGEIVGLIGLNGAGKSTT
                     IKHIIGLLEASKGTIEINHLSLKKNTEEYRKQIGYIPETPSLYEELTLREHIEVTAMA
                     YDIPMDIAMKRAEKLLETFRLSNRLDWFPANFSKGMKQKVMILCAFLIEPSLYIIDEP
                     FLGLDPLAINALLELMVEMKKEGAGILMSTHILATAEKYCDRFVVLHNGEIRADGSLE
                     MLRKEFQLPDSSLDEIYIALTKEQAHE"
     gene            11235..12446
                     /locus_tag="IWO89_000671"
     CDS             11235..12446
                     /locus_tag="IWO89_000671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAQ0455447.1"
                     /translation="MSSFFQKRLARHQKKMMKYMRYVLNDHFVLVCLFLIGGVGLYYS
                     NWLKTLQPPFAIGGLIISVFWMLCLFVGKMATFAEAADIVFLLPKEKEMQTYLERAFR
                     YSCIFPLIFLILACGLAMPLVVVSTEQSFSMFFVYLIILWSLKFSHLQIKRAELFRLD
                     QQVIRYWKLGWFFSSLIFLFLSFYWLIWAGIAGALVQASLYYWFLWKQQEKGLDWEKM
                     VHVEEHRLHQIYQFINLFTDVPEITAKIKRRRYLDPILTHIKRESKNTYLYLFARRLA
                     RGSDFSGLYFRLVIIGGLLIAGVVDFYFSLIIGFLFLYLIGFQLLPLANQFRYMVLVQ
                     LYPIHKNQKKQAIQYLLTWVLIIASVIFGLIAAVVLDGQQRWVPLAAYLIVTMLFVKL
                     YVPTRLKKMSD"
     gene            12653..13432
                     /locus_tag="IWO89_000672"
     CDS             12653..13432
                     /locus_tag="IWO89_000672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase family protein"
                     /protein_id="HAQ0455448.1"
                     /translation="MEFQLDQEWRLRPIKGDTGKTYIGMRDEDKVFIKRNTTPMLAAL
                     SREGITPKLVWTKRTGNGDTLTAQEWLEGRLLTPNEIGQRNDVIDVLYHLHHSNSLKT
                     MLTRIGGRVMTPEKMLQKYEKELPEELQKNQFLQIVHRFLREQIPSIDKENLSVVHGD
                     VNHRNWLVCKNYLYLVDWDSIMFADPAVDIATILGNYVPLSSWNQWLISYGIRPTDEM
                     LEKFHWYAAMNLLQEITRYCKREDHRRMNEEILQLKRIFSG"
     gene            13502..14164
                     /gene="trmB"
                     /locus_tag="IWO89_000673"
     CDS             13502..14164
                     /gene="trmB"
                     /locus_tag="IWO89_000673"
                     /EC_number="2.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(46)-N7)-methyltransferase TrmB"
                     /protein_id="HAQ0455449.1"
                     /translation="MRVRNRPGAAEMLAAHPNFVISDPTLWKGKWNELFENDHPIHIE
                     IGMGKGQFITGMAKAHPEINYIGVEMQVSVVSIALDKLIEQPLPNLKLLHVDGSALTE
                     YFADSEVDQIYLNFSDPWPKKRHEKRRLTYKTFLAVDEQILRPNGEIHFKTDNQGLFE
                     YSLASFSQYGMILKQVWLDLHQSQFEGNIMTEYEEKFSSKGQRIYRVEAQFQDKTKKK
                     SL"
     gene            complement(14262..16016)
                     /gene="ade"
                     /locus_tag="IWO89_000674"
     CDS             complement(14262..16016)
                     /gene="ade"
                     /locus_tag="IWO89_000674"
                     /EC_number="3.5.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine deaminase"
                     /protein_id="HAQ0455450.1"
                     /translation="METTLRKYIEVASKKKPADLVIKHAKIVNVFTKEIMEKDVAICE
                     GMIVGIGEYEGKSVHNANGQYLVPGFIDGHVHIESSLLSPSEFSKVSLLHGVTTVVTD
                     PHEIGNIAGSTGLEFMIEDARQTLMNIFFMLPSCVPVTPFETNGANLNAKKLAPFLQK
                     PEVLGLAEVMNYQAVANNETDILEKIQLMHQHKKKIDGHAAGIGMEELNVYPAAGIRT
                     DHEATTAKEAKERLDLGMYLMVREGTVAKDLASLLPAITPENSRRCFFVTDDKLINDL
                     VKEGSIDHIVRQAIALGIDPLQAIQMATLNAAECFDLKTLGAIAPGYQADFFLTDDLT
                     KLPIHEVFYKGTLVVQSGQLQDHLFADHSNSYTCCLPKLNAQPLNPHSLELLLSSSQA
                     HVIEIIPNSLVTKDLVEKVDRQGERFIPSIEKDQLKIAVIERHHQTGNIGLGIVKGFQ
                     LKQGAIATTIAHDSHNLVVVGTNDEDMLYAANLLIQKGGGMTVVNKQQEIACVSLPIG
                     GIMTQEPFSIVYEQLNELITKAHQLGAAKTFDPFLTLSFLTLSVIPELKITDKGLFSF
                     SRFQLIPPCGKTDEINTF"
     gene            16148..17077
                     /gene="cpaF"
                     /locus_tag="IWO89_000675"
     CDS             16148..17077
                     /gene="cpaF"
                     /locus_tag="IWO89_000675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Flp pilus assembly complex ATPase component"
                     /protein_id="HAQ0455451.1"
                     /translation="MEIKRLSDQMISESFAKHAQDVYILPASEVFYIYFRYGKKRTFW
                     KKVSSADGQQLISRFKYLGQMDIGEKRKAQLGAFTYSLEKQEVRLRLSSVGDYLQRES
                     LVIRLLYGTFETPHRCFSSRDFATILQKTVRRGLYLFSGTVGSGKTSLMYRLALEEKL
                     QVITIEDPVEIEEPFFLQLQINPKIAQTYDTLLKLALRHRPDLLIIGEIRDESTAHAA
                     VRAALTGHRVFATVHARNLEGTLVRIHELTKKKEELSECLAGVIYQELLEDANHHPSV
                     LWSYQFMEEESVKKSWEESYEEAQENIWQSELF"
     gene            17034..18083
                     /locus_tag="IWO89_000676"
     CDS             17034..18083
                     /locus_tag="IWO89_000676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence protein ComGB"
                     /protein_id="HAQ0455452.1"
                     /translation="MKKLRKIFGSQNYSKGLTRSQQNQFAQTMGELLLSGFSLQESLY
                     LLLTIRSFPPVILANIQHQLKEGQAFSEVLSQIGYGAEQLVQIELAERHGNLPTTLLE
                     IAKQFRLVEGFRNDLKKLLAYPMLLLTFLLGILFSMRLFVLPQLIGSGMVMQEHWGIQ
                     LIQVFHWYVLLFILAVTLLALLVRYRLMKLSPVDQSEWISRQLLIGPIYSLYQSAYLS
                     LEIGKLFHEGFELRQIIVCLKETKQESLIQHLASKLIQGLEAGMSLAEQFQNYRFLSE
                     EFSKIVLQGEAKGSLGKELIFYSEWTRKQLFKRLHHWMHFIQPIIFLGVAALILLVYA
                     AVLLPVYGSIEEVLP"
     gene            18080..18358
                     /locus_tag="IWO89_000677"
     CDS             18080..18358
                     /locus_tag="IWO89_000677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HAQ0455453.1"
                     /translation="MKKRKISYYSGFTLIEMLIVLLIISVLVLLFVPNLSRYRNHVDQ
                     ESREAIIQLVDTQKELYALQNNGRVPTVEELLNEGYIKREHAEIYQRP"
     gene            18333..18803
                     /locus_tag="IWO89_000678"
     CDS             18333..18803
                     /locus_tag="IWO89_000678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein"
                     /protein_id="HAQ0455454.1"
                     /translation="MQKSISGLEGKVLASGYTLFESLLLILLLTTILSFPVLAFSAWK
                     NELAVYQFFGQFEKRIYSTQKIAIVNQIQTGFYWNSEENQIIFHVPNPDKINWEVLDI
                     PDEITLKRHASITFAARTGNESSLKAYQFYWEEKERTITYQFQIGSGRYTKRIE"
     gene            18820..19122
                     /locus_tag="IWO89_000679"
     CDS             18820..19122
                     /locus_tag="IWO89_000679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0455455.1"
                     /translation="MESLTALMIASVCVYLVSFGQLFLLQAEKKSGEELLFIRMIYED
                     IKTQRMYHKRPERREEMIPYDIQIVDNNGVIEKVFVNMEKEAISIEKTSNSSIYAD"
     gene            19139..19510
                     /locus_tag="IWO89_000680"
     CDS             19139..19510
                     /locus_tag="IWO89_000680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComGF family competence protein"
                     /protein_id="HAQ0455456.1"
                     /translation="MILSSLLLTFNLLIQQTIQVSEFLQRKDQKEWLIFLAQLEEELK
                     SSHNVSVKNQQLHYTIEDKQYIIERYQAMIRKRRTSGGHQPMLTSITELQLLEKEHTI
                     SFYVHFENGEDGYAIWTKNDQ"
     gene            19482..19841
                     /locus_tag="IWO89_000681"
     CDS             19482..19841
                     /locus_tag="IWO89_000681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0455457.1"
                     /translation="MPYGQKTISRKCYKGSILFSVLLLFLLCTFLLMAALESYRLSMD
                     LNIRIKNYYIAKIMKQMALSEQSTLAESSEGVFYYTTGSVTYQWVQQSLFLEVEVSPF
                     IFRFTEEVKNDTDTSTE"
     gene            20013..21020
                     /locus_tag="IWO89_000682"
     CDS             20013..21020
                     /locus_tag="IWO89_000682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HAQ0455458.1"
                     /translation="MLPEKMEQGFNQNLEAIQLLQNALGTSFLEAYVENAENLIDDYQ
                     VRVVDGVPTKETTQRITALYEELKKLSFEPEEWRRLSQLLLLKGSQTEHLQANHQLTP
                     DSIGFLFVFLIEQLYTNKKAPVKILDIAAGMGNLLLTVLLNLSNAGYQTEGIGVDIDD
                     TLLAVAASTSDLTQANVQYFHQDGLQELLIDPVDFAISDLPIGYYPNDEKAKEFLTST
                     EEGHSYAHHLLLEQSMKYVKPDGFGLFLMPSGFLETDQSEEIKKWFKEEGYLQGMIQL
                     PDELFRNKQSQKSILILQKKGPQAQQVKEVLLVKLASLKEPEKVTEFFNEFKNWKSSA
                     L"
     gene            21045..22229
                     /locus_tag="IWO89_000683"
     CDS             21045..22229
                     /locus_tag="IWO89_000683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate kinase"
                     /protein_id="HAQ0455459.1"
                     /translation="MSKTIAINAGSSSLKWQLYQMPEETVVAKGIVERIGLQDSIFTI
                     KYGDNQKFEQIMDIENHEVAVKMLLDQLIELQILSSYDEITGVGHRVVHGGETYGDSV
                     VIDEKVMDRIAELAEFAPLHNPANLMGIKAFKKILPDILSVAVFDTSFHSTMPKQNYL
                     YSLPVSYYEDFKVRKYGFHGTSHRYVSERAAKLLNKPIEELKIITCHLGNGVSITAVD
                     GGKSMDTSMGFTPLAGVTMGTRSGDIDPAVLPYLMEKLEIDIDEMINVLNKKSGLLGL
                     TGLSSDMRDLEKNYEKEHIRLAYDIFVDRIRKYIGGYVTTMNGVDAIVFTAGIGENDG
                     HVRSEIIKGMSWFGCEIDPALNELRGEEVDISTKESKVRVLVIPTDEELMIARDVERL
                     RG"
     gene            complement(22666..24249)
                     /locus_tag="IWO89_000684"
     CDS             complement(22666..24249)
                     /locus_tag="IWO89_000684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HAQ0455460.1"
                     /translation="MIIQAISIKKTIGGNPLFEDLSCQIEAGEKIALVDINGTGKSTF
                     LQILTGREGVDSGVISRKKGLKIGTVEQELTVSEATVNHYLLHSAAEIQDLKQQMSRY
                     EALMTEPDTNLEKCLARYGELQHRFEELGGYVLEDRIAAILQGLGIPHCQEARLTELS
                     GGERVKVALAKILAADADLLLLDEPTNHLDLNSIRWLENYLQNTKKSVLVVSHDRQFL
                     DQVTTKTWELEEGALIEYPGNYSRFRVLKEARLAELTKNYELQQKEVQRLKVMIRRFR
                     QWAHEGDNESFFKKAKELERRLAKLTLVKPPPPPKNRLQSLSNGGKSGKEVFIIQNLH
                     QQYADQVLFKDSSFAVYRGDHLAIIGDNGAGKSTLLKLLLGEEKPYGGTIKLGSSLQI
                     GYLPQQLQFADPDSRLLAYAITLTGNEEVARRQLAKSGFYQSDVGKRIKDLSGGEKIR
                     LALLKLFLEKINVLILDEPTNHLDSYAREEIEEMLQDYQGTLLAVSHDRYFLQQHFQE
                     ALVIDQEQISRQPLGQLID"
     gene            complement(24827..24970)
                     /locus_tag="IWO89_000685"
     CDS             complement(24827..24970)
                     /locus_tag="IWO89_000685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="portal protein"
                     /protein_id="HAQ0455461.1"
                     /translation="MEVSDESDTKKYEVSMADTDFIAKLKFYFGFGYREKASATFGKS
                     FKN"
     gene            complement(25192..25671)
                     /locus_tag="IWO89_000686"
     CDS             complement(25192..25671)
                     /locus_tag="IWO89_000686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HAQ0455462.1"
                     /translation="MLQQYKKIMVAVDGSDEAELAFKKAVNVAIRNNGELLLAHVIDT
                     RSFQTVSSFDGMLAEQATEMAKQTLADYESNAKKAGLNNVTSVVEYGSPKQIIAREIP
                     EDNQVDLIMLGATGLNAVERLFIGSVSEYVIRNAACDVLVVRTDLENQLPAKDHTKA"
     gene            complement(25744..26028)
                     /locus_tag="IWO89_000687"
     CDS             complement(25744..26028)
                     /locus_tag="IWO89_000687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0455463.1"
                     /translation="MIRILMIIATLVLLFVSYYLFNKQDIFFVLINKNDKNQGFLQFY
                     GAAYAVLGVMGILTAFFNQRFIALVFLLIVIIVSATFSIRFAKKIAEPKQ"
     gene            complement(26106..26300)
                     /gene="ssrS"
                     /locus_tag="IWO89_000688"
     ncRNA           complement(26106..26300)
                     /ncRNA_class="other"
                     /gene="ssrS"
                     /locus_tag="IWO89_000688"
                     /product="6S RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00013"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00013"
     gene            complement(26372..27658)
                     /locus_tag="IWO89_000689"
     CDS             complement(26372..27658)
                     /locus_tag="IWO89_000689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication-associated recombination protein A"
                     /protein_id="HAQ0455464.1"
                     /translation="MQQPLAYRMRPRTIDEVVGQQHLVGEGKIIRRMVDARMLSSMIL
                     YGPPGTGKTSIASAIAGSTNYAFRMLNAATDSKKDLQVVAEEAKMSGTVILLLDEVHR
                     LDKTKQDFLLPHLESGRIILIGATTENPYITINPAIRSRTQIFEVKPLTEQDIQLAVE
                     HALRDKERGLGQQAIQLDEDALLHLSRATNGDLRSALNGLELATLSTPSDKEGRIHLT
                     LSIIEECVQRKALTHDKNGDAHYDVISAFQKSIRGSDVDAALHYLARLVEAGDLASIC
                     RRLMVIGYEDIGLGNPAAAARTVNAVLAAEKLGLPEARIPLADVVVDLCLSPKSNSAY
                     MALDAALADIREGKAGDVPDHLRDSHYKGAKSLNRGVGYQYPHHFDQAWVNQQYLPDK
                     LKNAQYYQPKDTGKYEQALGQQYYRIKEWKKHPPKN"
     gene            27796..28467
                     /locus_tag="IWO89_000690"
     CDS             27796..28467
                     /locus_tag="IWO89_000690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase"
                     /protein_id="HAQ0455465.1"
                     /translation="MTDKQKIDEFFWNKTTPEVARDLLGMYLEHDTPEGRLAGYIVDA
                     EAYLGPEDEAAHSYGLRRTPRVRAMYEKPGTIYLYTMHTHRILNIITQPEGIPQGVMI
                     RAIEPAAMIDQMSKNRGGKTGPDISNGPGKLVEALAIPQELYGQSIADSSLRLVFEKK
                     KTPKKINALPRIGIPNKGVWTEKPLRFVVSGNPYISLQRKNQIEKDWGWRKENEKEGS
                     INIFR"
     gene            28433..28921
                     /locus_tag="IWO89_000691"
     CDS             28433..28921
                     /locus_tag="IWO89_000691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3013 family protein"
                     /protein_id="HAQ0455466.1"
                     /translation="MKKKDLLTYLDEIIEKQVTDYDVALDWDTKNHTIEIVVRLFAEN
                     KAQFEIDDAEGIVSQEPIIEFEDGVLLFNPQKSVFDERNYLAVIPYEGKKGLAKSVAD
                     SLVEYLNEVLAQGQSDLLDFLDEDDDEAVFELHWDNQQLAELVEVKQQNEADTYLPYP
                     SY"
     gene            28934..29881
                     /gene="prmA"
                     /locus_tag="IWO89_000692"
     CDS             28934..29881
                     /gene="prmA"
                     /locus_tag="IWO89_000692"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HAQ0455467.1"
                     /translation="MKWNEVKVETESEAVEAVANILMEAGASGVAIEDSLDVEHFQSD
                     QYGEILDKNDFTSLKEGALVMAYFPETTFLPEILPFIKDRITQLPEFGLAIGKNKVTV
                     SEVEESNWATAWKKYYHPVRITRMLTVAPSWENYQTEDPLEKIITLDPGMAFGTGTHP
                     TTSLTLTALETVLRGGETLLDVGTGSGVLSIAAKYLGAKDVYAFDLDEVAVRSAKENM
                     DMNEIAKDVHVSANDLLTGIEIESDVIVANILADIILLMIPDAWRLLKQTGTLIVSGI
                     IEEKKQMVLDAMLEQGFIVDQIFQQKDWYAIILKKPEVE"
     gene            29882..30646
                     /locus_tag="IWO89_000693"
     CDS             29882..30646
                     /locus_tag="IWO89_000693"
                     /EC_number="2.1.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (uracil(1498)-N(3))-methyltransferase"
                     /protein_id="HAQ0455468.1"
                     /translation="MQRYFLKESYESKDYFVLEGENYHHIVRVMRMEPEDEVFLAFND
                     QIAIVAEITEITEEAVYLKEIKKEENQKELPSSITIASGYPKGEKLDWIVQKSTELGA
                     QAFLGFPASASVVKWDAKKRMKRQQRLEKIAQEAAEQSHRQIVPSVELLETKQDLFER
                     LDDYDVVLVAYEESAKQGEKSNAAKILSETKPDSRILAVFGPEGGLSRDEVDYFVEHK
                     GILCGLGPRILRTETAPLYLLSAASYQWELMNPSFS"
     gene            30879..33092
                     /locus_tag="IWO89_000694"
     CDS             30879..33092
                     /locus_tag="IWO89_000694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010749038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional (p)ppGpp
                     synthetase/guanosine-3',5'-bis(diphosphate)
                     3'-pyrophosphohydrolase"
                     /protein_id="HAQ0455469.1"
                     /translation="MTKEEILTGPGVIKLVSQYMGPEHVAFVEKACEYATAAHDGQFR
                     KSGEPYIIHPIQVAGILADLKMDPHTVATGFLHDVVEDTEITLEDLREEFGDDVAMLV
                     DGVTKLGKIKYKSHEEQLAENHRKMLLAMAQDLRVIMVKLADRLHNMRTLKHLREDKQ
                     RRIAQETLEIYAPLAHRLGISRIKWELEDTALRYLNPKQYYRIVHLMQTKREEREKYV
                     SGTVEDIRIATEELGIFAEIYGRPKHIYSIYRKMKDQKKQFNEIYDLLAIRVIVDSIK
                     DCYAVLGAIHTKWKPMPGRFKDYIAMPKANMYQSLHTTVIGPAGNPVEIQIRTQEMHE
                     IAEFGVAAHWAYKEGKNEKVEPDGMTKQLSWFHEILELQDESYDASEFMEGVKGDIFS
                     DKVYVFTPKGDVTELPKGSGPLDFAYSIHTDIGNKTTGAKVNGKMVQLDYKLKNGDII
                     EIMTSPNSFGPSRDWLKLVATSKARNKIKRFFKAQDREENVIKGHESVVKCITDLGFT
                     PKDILTKNKLQEALDRFNYQTEDDLYAAVGYGEVSPLTMANRLTEKERKEQKIEQQKQ
                     EAEEIMNQPKKEPDKMKVRHEGGVVIQGVENLLIRISRCCNPIPGDDIVGYITKGRGI
                     SIHRRDCPNVQPDKPNVAERLIEVEWEDTSNTRKEYDADLEIYGYNRSGLLNDVLQTV
                     NALTKNLNSVEARTNKDKMATIHLTVGIQNLSHLKSIVDKIKAVPDVYSVRRTNG"
     gene            33103..33549
                     /locus_tag="IWO89_000695"
     CDS             33103..33549
                     /locus_tag="IWO89_000695"
                     /EC_number="3.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-tyrosyl-tRNA(Tyr) deacylase"
                     /protein_id="HAQ0455470.1"
                     /translation="MRAVVQRVSKAAVSVDQQEIGKIGYGLLVLLGIHETDTQKDVDY
                     LVKKIAQMRIFEDEQGKMNISVEDVGGQILSISQFTLFADTKKGNRPSFVEAARPETA
                     IPLYESFNEGIRSRGITVATGQFGADMSVSLVNDGPVTIIIDSKNK"
     gene            33720..34979
                     /locus_tag="IWO89_000696"
     CDS             33720..34979
                     /locus_tag="IWO89_000696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HAQ0455471.1"
                     /translation="MKEVPVVDIKITDLKKLYQKGYNMLEELRHEADAPVVKAKIFNK
                     EAITIYGSSAAKVFYDPRNFKRKGAMPKLVLKTLFGQGGVQTLDGAAHHHRKNIFMDL
                     MTPERMEDYHRILDKNLTQALEAQHGQFELFDLSKMVFFTSICEWAGINLSAISKDEV
                     EKLAEYQISMISGTFTSPIDHIKGVENRKKSEKWAQGLIEEARQNPVAGKENVALYAF
                     ANATDLDGQLLPLEVAAVELLNIIRPTVALTVWAALMGHALFSRPDLYQQLKNDFSTL
                     QDPFIQEMRRYYPFFPMLPAISLKEVEVDGYRIPEGSWVILDLYGTDHDERTVEAPDS
                     FMIKRYVGKAKDISYKEEYEMIAQGGGNFRQMHRCAGEWITLHSLRVFSDQLVNKFEF
                     SVPEQDWTIPFNQFPTYPNSRALLYKN"
     gene            complement(35068..36015)
                     /locus_tag="IWO89_000697"
     CDS             complement(35068..36015)
                     /locus_tag="IWO89_000697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HAQ0455472.1"
                     /translation="MAEIRIGHSQVYAEQLGLGANAVGGHNLFDGLEDETGKQVVRTA
                     LNSGINLIDTAYAYGNGRSEELIGEVLKEKEYDRSRVVIATKAAHVPNKGRTFDNSPE
                     FLKQSVEDALKRLQTDYIDIFYIHFPDESTPKNESVATLHELKEAGKIRAVGVSNFTL
                     EQLKEANADGYVDVVEDKYSLIHRQAEKELFPYLEKNKISFVPYFPLASGLLTGKYEL
                     GEEKQFGEGDPRKRNPDFQGERFREILTAVDVLRPIAKRYQATPAQLVLAWYMKNPRV
                     SVVIPGAKRPEQVSDNVQALDLHLSNEDYQTIDEAFRGF"
     gene            36409..37701
                     /locus_tag="IWO89_000698"
     CDS             36409..37701
                     /locus_tag="IWO89_000698"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HAQ0455473.1"
                     /translation="MSFQRPKGTNDILPGESEKWQFVEETARLLFRDYQYNEMRTPIF
                     EHYEVISRSVGDTTDIVSKEMYDFYDKGERHVTLRPEGTAPIVRSFVEHKLFGPEYAK
                     PFKTFYMGPMFRYERPQKGRLRQFHQIGVEAFGSENPATDVESMVMALDFFKQLGITQ
                     IRLVINSLGDKETRQAYRQALIDYLLPFESELSEDSKRRLHENPLRVLDSKDKRDQKF
                     VAEAPSILDYLSEPAQKHFDTVVSMLDALAVPYEIDSNMVRGLDYYTHTIFEIMSDAP
                     KMGAQATICAGGRYDNLVEELGGPSTPGFGFAMGIERLLLTMEAEEVVIPAFNELDAY
                     VVALGDETNIEALKVVQAIRNFGFSADRDFMNRKAKAQFKTADKANAKLVLVIGEDEL
                     ASQTINVKAMASRKEKSFPLEEIYEHFDEVYDEMTLFE"
     gene            37716..39485
                     /gene="aspS"
                     /locus_tag="IWO89_000699"
     CDS             37716..39485
                     /gene="aspS"
                     /locus_tag="IWO89_000699"
                     /EC_number="6.1.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016251448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HAQ0455474.1"
                     /translation="MAQRTIYCGLVSEQQLEQTVTLKGWVQKRRDLGGVIFIDLRDRE
                     GIVQIVFNPEINKEAWEIADKCRSEYVIEVTGEVRRRGENAVNPKMATGEFEVIASEI
                     VILNQAKTPPFQIEDDQAISDEIRLKYRYLDLRRPKMTKNIKLRNATTKAIRHYLDDN
                     DFLDIETPYLGKSTPEGARDYLVPSRVHAGHFYALPQSPQLFKQLLMNAGFDRYYQIV
                     RCFRDEDLRGDRQPEFTQVDIETTFLTAEEIQTYTEGLIAKVMKEVRGIEVTLPFPRM
                     TYDEAMARYGSDKPDTRFDMELIDLSDTVKEVEFKVFQMALENGGVVKALNAKGAADR
                     YSRKDMDQLGQYVGQFGAKGLAWLKVEEDGLKGPIAKFMGEATEAIIKATDAKPGDLL
                     MFGADKSEIVAAALGAIRTRLGKELGLIDESKFNFLWVTDWPQFEFSEEEGRYVSAHH
                     PFTMPKEEDIPLLAEDPAKVYAEAYDIVLNGYELGGGSIRIHTRELQEKVLETLGFTK
                     EQMEEQFGFLLTALDYGFPPHGGIALGLDRFAMLLAGEDNIREVIAFPKNGKAADVMS
                     DAPSMVSPLQLFELNIDVTNIEE"
     gene            39594..40468
                     /locus_tag="IWO89_000700"
                     /pseudo
     CDS             39594..40468
                     /locus_tag="IWO89_000700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723872.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
     gene            40734..42617
                     /locus_tag="IWO89_000701"
     CDS             40734..42617
                     /locus_tag="IWO89_000701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyltransferase"
                     /protein_id="HAQ0455475.1"
                     /translation="MEKQKRLKNSRYITGFDGLRSLAVVGVIFYHLLPTSMKGGYLGV
                     PIFFVVSGYLITDLLRQEWEQNGKINIWQFYVRRMKRLYPALVFMLVTASAYITLFQR
                     GLLNNLRGVVVSSLLYVNNWWQINNGLSYFDRFANESPFTHIWSLAVEGQNYLVWPIL
                     FVLLMVFVRKRKWIFYTVLGTSFLSAILMMILYTPGGDPTRVYYGTDTRLFSIWLGSA
                     LAFVWPSTRLKKNIPKQAKNILNIAGGISLLALILCFFFLDDHYNFIYYGGMLLVSLL
                     CVVLVAVTAHPGASLNRWLTNPVFTWIGKRSYGIYLYQFPVMIFYEAKVTDIADHVLL
                     HTLVEITLILIISELSYRYVERPLARFDYGRTFQVMKGWFSKPIISKSKPWVVPGTLL
                     VAIALVGFVTAPKNAVTADQKQLQAKIAESKKLAEETRNNSENTTSSVDQAILDKYEL
                     SKAQGNKAQKLELTAFGDSVMLDAATDLQELYPKVVVDGDVGRQLYASVPYIEKLKEQ
                     DLLKDTVLIGLGTNGSFSEAQFDSVMDAIGDRKVYWVNVRVPTKRWQNDVNAMLEQMT
                     KKYDNMTLIDWYSYSNDHSDWFYDDQVHPNPEGMTHYIHLVSEALLGGSSSTNSSETN
                     VTE"
     gene            complement(42692..42976)
                     /locus_tag="IWO89_000702"
     CDS             complement(42692..42976)
                     /locus_tag="IWO89_000702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HAQ0455476.1"
                     /translation="MANEHYFYVLFCKDQTFYGGYTTDLSRRLEEHNNGTGAKYTRLQ
                     KRRPVKMIHAEVFSSRSEATKAEAAFKKLTRKQKERYLQTHSTVLLPESI"
     gene            complement(42966..43700)
                     /locus_tag="IWO89_000703"
     CDS             complement(42966..43700)
                     /locus_tag="IWO89_000703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA1(Val) (adenine(37)-N6)-methyltransferase"
                     /protein_id="HAQ0455477.1"
                     /translation="MLHEDERIDQLYAEDIKIIQSSKVFSFSLDAVLLAHFSRVPKKG
                     KIVDLCAGNGAVGLFLSKRTQAAIDAIELQPRLADMARRSIALNHLNEQMTVHTIDLK
                     DSLSVVRHNSCDLVVCNPPYFKGLPTNKTNPNKHLAIARHEIHTTLEEVIDISSKLLK
                     TNGRFTMVHRPDRFLEIIQVMEDYRIAPKRIQFVYPKPGKEANILLVEGIKEGKLDGF
                     KVAPPLITYNMDGEYTAEIREMLYGK"
     gene            43829..44452
                     /locus_tag="IWO89_000704"
     CDS             43829..44452
                     /locus_tag="IWO89_000704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-acyl-sn-glycerol-3-phosphate acyltransferase"
                     /protein_id="HAQ0455478.1"
                     /translation="MFYRFMRGVVRVLLALVNGNARYENKEVLPQDENYILVAPHRTW
                     WDPLYLAVAAAPKEFSFMAKEELFKNPVLRFILTRSNAFPVKRENPGPSVIKTPVKIL
                     KSTNLSLIMFPSGTRHATELKGGMALISRMSKAKIVPAVYQGPLTLGDLFKRQRVTVR
                     FGEPIDLSDIKKTDKEGMAEIERRTQAAFDKLDKEVNPDFKYEVKKK"
     gene            44644..45765
                     /locus_tag="IWO89_000705"
     CDS             44644..45765
                     /locus_tag="IWO89_000705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease SbcCD subunit D"
                     /protein_id="HAQ0455479.1"
                     /translation="MRFLHTADWHIGKKLQGYDLIEDQRHVLSSILDIAKEEKVEAIV
                     IAGDLYDRSVPAVDAVELYNELMVDWNLKEKIPIFAISGNHDSSTRLSAGTPWFSYSN
                     YFLRTQLAEAFEPVEFKNVQFFFLPYFEPIAARLYFEDDNIRTIKQAVAKVIDKMQEL
                     FDSEKKQVLISHFFVMGSVKSDSETKIEVGGLDSIPGELLTPFDYVALGHLHNQAALR
                     QDNARYSGSPLKFSLSEINQKKGVWIVDLTTGLELNFREIEPLHDIEEITASFKELLD
                     PAFYQSVNRENYIYIYLTDRTIIPNMMNQLRQIYPRILGIERANGRAEQTKRKRKVLE
                     MKDPQKLAEAFFSEMTEEQLTANQEKWLGETLQEIMEME"
     gene            45772..48900
                     /locus_tag="IWO89_000706"
     CDS             45772..48900
                     /locus_tag="IWO89_000706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SMC family ATPase"
                     /protein_id="HAQ0455480.1"
                     /translation="MKPKKLQLKNFGPFINETVDFSRLTEAPLFLISGKTGAGKTTIF
                     DGITFALFGETSGRLRSGKEMRSLFATPDEETSVTFSFEHQQMSYEIERKPEQVLAKR
                     KGNGTKKQAAKVTLTIFDGKGKEIKQITKRTEADQLIRELMNLDAKQFSQIVLLPQGE
                     FRNFLVSSSSDKETVLRHLFGTQFFQQFNERLKEKAKQQQQKLDHLEQELHLLQKRFV
                     LVKEEEPAAGFEAVLAQWTNHQAILEKQITEEKEALTELQEKQKQLENSYYRFESLKK
                     SYEEKTILINKQKELAAQKDEIEEKKRWIGYYEFTQRLVEPIQRLQEIKVEKIELSQK
                     SSEQTKKLAMTTQNYEEWQKNEANRVRRQTKIKEAAQKLQNKQMMVPIVESLRSKKQD
                     AEQLTISLDKEELNAEKLYQNLAIYQERTRILQENLLDQEKLQEDRLAFGQLKYLDEK
                     QQEIEKQKAKDTGSLAQIREELKKATEEITKNSQLLKEQTEQAEQLKSQWAKQQIARL
                     QLFLRPGEPCLVCGSTEHPIQQLKHEQPNKEDILKVEQQLAEAESAVERTQQIIAKSE
                     AHENNLQQQLSDLEQKIMATNIQLAEQEEHLQQALKKAFPEITEQKTKTTLVVIEQSL
                     DRRKSKMANERIELEEIRQQAEKLEQQLAVYKQQQTEKNNQLQQLQGEIKSLEQQLQD
                     TDSNCLEEEIRRLEKQLEEDSNQLAVEQKAGVQLLQDLHTLRTQLDLQHEQIEKIDEK
                     IEKEQERIAKKLESQSYFSTIEEICAFAQQQAQYKENQRTVTSYHEEQLVTADRLRQL
                     ENIQLTETFPDTEEISQQLEEIRRMIEIKQEKIYGLQEQKISNQKIYDECLSIYHTSQ
                     TKLDELTQLQQLSQTFNGENPKKTSLERYVLQVYLQEVLQVANDHLQRLTKNRYQFEL
                     ADTIGSYRGKTGLEINIYDDQAGMSRSAHTLSGGESFIAALSLALALADVIQTQAGGV
                     AIDALFIDEGFGSLDEEALEMAMEALETIESEGRMIGIISHVRELKERVLQQIRIDTE
                     GSGQSKIRYQLG"
     gene            48919..52113
                     /locus_tag="IWO89_000707"
     CDS             48919..52113
                     /locus_tag="IWO89_000707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HAQ0455481.1"
                     /translation="MKWSIPEKVIARGRQYLQEGRVLSVVPDQEENVWHAEVLGSELY
                     MVDLDGTAKEIDYCQCPYWEEHKYCKHTVAVELYLRSKNLSRIMKKDQPMLEPVKATS
                     EGEIIAKGFSRLKTQNNPQAVQPLIIEYQVETIETNQYHPELSILALYLRIGSLASPK
                     KTYIVKNIYDFLQAYTEKETYTVNKQYQFRLDKRAFQKEDQKILTHLAGSAQTHQLLG
                     TNGLQVKGKLDKRYLLLPVEQARFLLEQMNQTTRLHLQINEQNDQGIQFSDQNKPLSF
                     KVEKFGEDYLLQALNDFDFFFMHYQWGVIQGTFYSLTKYQQTVYQTWKQLIRRLEKPE
                     VVFKKKELPSLFKEIIPLLSEIGDVAIAPAVEEEVADIPVEFIFFFRKAKGKIQARVD
                     FVYGEVIYSTDPKHEVSTEQAFQVLRDTVEEKRVLDLFRMYRYQKNETGYERPLPSGE
                     ELYAFFRTELSVFRQFGEVRLGRKLRELYLDAHKHQPKIEVTETGSWLDIRFDVTGIE
                     EQEIDHVLQSLLRNDAFYTLENGQVLSFDSEEFQQTSQVLQQLRESIRTEEGTIHVPK
                     NQGLIIQNQLEKSNATFSESFQTMVQDLIHPERYQAQLPKGLNATMRDYQKQGFRWLK
                     MLGHYQFGGILADEMGLGKTLQTIAFLLSEKEERKSFSALIVAPASLIYNWQAEVRKF
                     APSLSIQVINGNKKEREELLAKDTDIRVTSYASLRQDLANYQSQKIDYLILDEAQMVK
                     NSSTKTAQALRELAVPQRFALSGTPIENNLEELWSLFATIMPGFFPTKTKFRELAAEE
                     IAQMIRPFILRRDKQTVLKDLPEKTEMNLYSALTEEQKTVYLAYLRQMREEITSMDSE
                     AFKKNRIGILAGLTRLRQICCDPRLFIEDYQGGSGKLEQVKDLLVAAKENKRRVLLFS
                     QFTGMLTILQEELAELGISTFYLRGSTKPQDRLSMVDAFNAGEKDVFLISLKAGGTGL
                     NLTGADTVILYDLWWNPAIEEQAAGRAHRIGQKNVVEVWRMISEGTIEERMDSLQQEK
                     RELFQKVIQGNEEQLTKLTEEDIRMILSVGEIEE"
     gene            complement(52259..52966)
                     /locus_tag="IWO89_000708"
     CDS             complement(52259..52966)
                     /locus_tag="IWO89_000708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HAQ0455482.1"
                     /translation="MIKSVVFDVDDTMYDQQQPFRNAVKRVVPLVSDADMHPLYIRFR
                     HHSDENFPKVMAGDWTLEYMRAHRISQSLKDLDYPHITEEDGLLFQKIYEEELDNICL
                     HEEVKKTLDFLKEKNVPLGIITNGPTDHQTKKLKQLQLNNWIPSRNMLISQATGFQKP
                     EKEIFQLAEKEFHMLPEETLYVGDNYDNDVLGAKSANWQALWFNHRERKIEQTPICDI
                     EISSFDQLLETMKTIFV"
     gene            53731..54129
                     /gene="spxA"
                     /locus_tag="IWO89_000709"
     CDS             53731..54129
                     /gene="spxA"
                     /locus_tag="IWO89_000709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator Spx"
                     /protein_id="HAQ0455483.1"
                     /translation="MLTLYTSPSCTSCRKARAWLQEHQIPFVERNIFSEPLNSSELKA
                     ILQMTEDGTEEIISTRSKVFQKLNMDLDDLPLQELLELVQNNPGLLRRPIMIDDKRLQ
                     VGFNEDEIRRFLPRDVRQLELRQAQLMAGL"
BASE COUNT        17670 a         9488 c        11392 g        15605 t
ORIGIN      
        1 cagccaccat ttaaagagtg cgtaatagct cactagtcga gtgaccctgc gccgaaaatg
       61 taccggggct aaacatatta ccgaagctgt ggagtacacc tttaggtgta ttggtaggag
      121 agcgttctaa gggcgttgaa ggcagatcgt gaggactgct ggagcgctta gaagtgagaa
      181 tgccggtatg agtagcgaaa gacaggtgag aatcctgtcc accgaatgac taaggtttcc
      241 tggggaaggc tcgtccgccc agggttagtc gggacctaag ccgaggccga caggcgtagg
      301 cgatggataa caggttgata ttcctgtacc cgttgttttt gtttgagcaa tggagggacg
      361 caggaggcta aggaatgcag acgatcggaa atgtctgtcc aagcagtaag tctgaagagg
      421 agtcaaatgc ttcttttctt aaggacaagc tgtgatgggg agggaaataa tagtaccgaa
      481 gttcctgatg tcacactgcc gagaaaagct tctagtgaga aaacagcggc ccgtaccgca
      541 aaccgacaca ggtagtcgag gagagaatcc taaggtgagc gagagaactc tcgttaagga
      601 actcggcaaa atgaccccgt aacttcggga gaaggggtgc tgatcatacg atcagccgca
      661 gtgaataggc ccaagcgact gtttatcaaa aacacaggtc tctgcaaaat cgtaagatga
      721 agtatagggg ctgacgcctg cccggtgctg gaaggttaag aggagtgctt agcgcaagcg
      781 aaggtacgaa ttgaagcccc agtaaacggc ggccgtaact ataacggtcc taaggtagcg
      841 aaattccttg tcgggtaagt tccgacccgc acgaaaggcg taacgatttg ggcactgtct
      901 caacgagaga ctcggtgaaa ttttagtacc tgtgaagatg caggttaccc gcgacaggac
      961 ggaaagaccc catggagctt tactgtagtt tgatattgag tgtctgtacc gcatgtacag
     1021 gataggtagg agccgtagaa atcggaacgc tagtttcgat ggaggcgctg gtgggatact
     1081 acccctgcgt tatggccact ctaacccgca ccactaatcg tggtgggaga cagtgtcaga
     1141 tgggcagttt gactggggcg gtcgcctcct aaaaggtaac ggaggcgccc aaaggttccc
     1201 tcagaatggt tggaaatcat tcgaagagtg taaaggcaga agggagcttg actgcgagac
     1261 caacaagtcg agcagggacg aaagtcgggc ttagtgatcc ggtggttccg catggaaggg
     1321 ccatcgctca acggataaaa gctaccctgg ggataacagg cttatctccc ccaagagtcc
     1381 acatcgacgg ggaggtttgg cacctcgatg tcggctcgtc gcatcctggg gctgtagtcg
     1441 gtcccaaggg ttgggctgtt cgcccattaa agcggcacgc gagctgggtt cagaacgtcg
     1501 tgagacagtt cggtccctat ccgtcgcggg cgttggaaat ttgagaggag ctgtccttag
     1561 tacgagagga ccgggatgga cttaccgctg gtgtaccagt tgttctgcca agggcattgc
     1621 tgggtagcta tgtagggaag ggataaacgc tgaaagcatc taagtgtgaa gcccacctca
     1681 agatgagatt tcccatttct ttaagaaagt aagatccctg agagatgatc aggtagatag
     1741 gtcaggagtg gaagtacagt gatgtatgga gcggactgat actaatcgat cgaggactta
     1801 accaataggt taaaagaaaa gaactcggaa aaaaacttat gtgacttcaa atccagtttt
     1861 gagtgaacaa aatttactca ataaaagata acaccacagt gtggtggcga tagcgagaag
     1921 gatacacctg taaccatgcc gaacacagaa gttaagcttc ttagcgccga ttgtagtgag
     1981 gggttgcccc ttgtgagagt aggacgtcgc cacgcaaagg caaaaagatg aactagaaat
     2041 agttcatctt tttttgttta aatcttttgc taagttgaac taatagagtc ttttatgaat
     2101 atcgaagtta gtttttcacc taacttcgtt ttttatagtt ggttattata aaatagtaat
     2161 atagctgatt tggattcaat tcatatttat ttataaatca aaaatacaga gaatagtata
     2221 taaatgtcct ttcctattct taaaatgatg tttgtactta cttaaattag gtataattag
     2281 tatatgtttt gtgagaaatt tagacttaca ataagcagtg aatttataat caacgtatag
     2341 gagacatgga tgaaatgaaa ctaattggta tagatttaga cggtactctt ttgaattctg
     2401 accagagcat tagtcaaaaa aatgcaagaa caatgaaaca tttaccggaa gactgttttc
     2461 cattcatttg ttctggaaga gaagtagaag acattaataa tattctcaaa aaatctggtt
     2521 taaggatacc agcagtaggg ttaaatggtt ctgttggatt tgatggagaa aaaaagatat
     2581 ttgagtcttc cttcgataaa aatgatgtaa aaaaaattga ttctgtccta tcgaaatttc
     2641 caaccaaaat atatacaaat ctcggaagtt atgaatctca gaattataaa aataaaatga
     2701 aaaaaatttt tcacgaggta gggagtgaat tttctatcaa agagctaaac tatgagcttg
     2761 agtatgaaaa atctatttgt tcaatccctt ataataatat tgaggaaatt atagaaaagg
     2821 atgctatcag catttacaaa ttttttattt ttattcctaa tcgtcaattg aaagctaata
     2881 tatttaacca tttgaaaaag atgctaaatg tgtctgttac tgaatcggca cctgtaaata
     2941 ttgaaatcgt acctcaaaat gtatctaaag gtgtggtgtt tgatcgtttg gaatcaatct
     3001 acaatttaaa gaaacctttg agaatcgcga tcggagatag tttaaatgac ttatctatgt
     3061 ttgaaagtgc agatattagt tttgcaatgg gaaatagtca tcaagaaatc atgaaaaaag
     3121 ctgattatgt cactgtttca aatgataaag acggagtatc tcaagcattt gaaagaattc
     3181 ttaccatata aactaattaa ggtagtatag aacatagaat tctaaatacg gaaaaatgga
     3241 gcataaattc cagtacaaat agtacttctt cagtgtcaaa cgaagcgaat ataacataga
     3301 tatttaggat tgtgacaaaa atcatgtcac aatctttttt tcgaataaac ttattttttg
     3361 aagtcggcca tttatcgtaa tttctttgca taatacgaac aaattttcgg ttattgatga
     3421 aaaaaaacat tgaaaatggt atgataaaag aaaaaaagga ggaaattttt aatggtgaaa
     3481 tttatccatg cggcagattt acatatggat cggtcatttg aaggactgac aactcttgat
     3541 aaaactgtac aggaaaaact attgaagacg aatctgaccg ttttatcaaa tatcattgaa
     3601 caggcaataa taaataacgt tgattttgtt ttacttgcag gagataattt tcatcaaaat
     3661 cgtccttcat taaaaataca gaaacatttt tcagaacaga tgaaacggtt agaaaagaac
     3721 catatacctg tatttataat attcggaaat catgatttct atcaaaaaga gcggtattgg
     3781 ttctcctttc ctaaaaatgt ccatttattt acatccgaaa cggtagagac aaaaaaaata
     3841 acgataaaaa gtggagaaac tgtctctctc agtggattta gttatcgtca accgtggatt
     3901 caaaaagaca aagtgatgga atttccttct agagaactca cagattacca tattgggttg
     3961 taccacgggg aacctggcgt ctctcaaaaa ggaaactatg ctcctttcca accctcaaag
     4021 atgcaagaaa agggatacga ttattgggct ttagggcata tccatgtgcc aacggtctta
     4081 aatgaaaagc aaacaattgt ttatccaggt gcgcctcaag gtcatactaa aaaggaacaa
     4141 gcttctacat ctattctact agttgaatta agtaaaggaa gttgccaaat acatccgatt
     4201 aaagtagcag aggtctattg gaagacaaag gaaatatcat tgaggaaagc aagaacgaca
     4261 aaagaggtca ttgttcacat tcgtcagcag ctttcaaaag tagaggatgg atttagttta
     4321 attgagataa aattaaaaga ttatgaccat ctcaatacag atgttttgga acggatacaa
     4381 tcaggagagc tattagacta tttgttagag gaattctcag ataggataaa tgatttcttt
     4441 atttggcgta tttctctcat agagaatact ttttatacga agaccccact tgctgcttct
     4501 gctaaattga tggaacagtt atttcaatac tatcagacac ctcaagattt ccaacaaatt
     4561 ttaaatgaag tatatagtca tcaagaagcg gctagaatat tgagcgaact gccggaatat
     4621 caagaagaaa cacttgaaaa agccaagcag ctgcttaatc aagatttttt atttgaggag
     4681 gatcaggaat gaagatttta aggatcgaga ttgctgcttt tggaaaatgg cgtcaaaaaa
     4741 gttttgactt ttattcaggc aatcagctga tctatggggg aaacgaagca ggaaaatcaa
     4801 cgatttatca atttattcaa gcaatattat ttggtttccc ttcaaaaggc cgtaaaaaaa
     4861 aggattatac accaaaagac ggctcagcct atggcggcaa aatatggctt aaacatcctg
     4921 tatatggtga atttgctgtc gagcgctaca agcagcaaaa tcgcggaaaa tcgaaggtgt
     4981 ggctggggga tcaagtggga agtgatgagt tactagaaaa actgatcgca cctttgacga
     5041 aagagttatt ccagcaggta tttactttcc agcaagagca gttgatggaa ctagatcatt
     5101 tgagagaaaa agagctacat gatgcgctga tttctttagg catcacgggc agttctcaag
     5161 tattccaaaa gcggcaagaa tattatcaga aagcacaaca attattcaag aaaaaaggcc
     5221 aaaaattact aatcaatcaa aaattaaatg aatggaaaga gctgcaagaa aaaattcatc
     5281 aaaaacaaga acaggaagct tattttggca agttgatcca acaagaacag gaatacgagt
     5341 cgcaacagca gcagttattg gcacaattaa aagaggcgaa caaacaattt tcattagcca
     5401 gacagcaaga acttaatttt tctttatatg aagaatggca atctctcaaa aaaaacagca
     5461 ctacaggtct tcctgatgag caacttcttt tagatctgga gtctttctcc aaacggtatc
     5521 agcaaacaaa tgagcgtttg gaagaattag aagcaacact ggaaaagcat agcgggatgg
     5581 atcagcagtc agccagatat tatttttatt tagagcagga gcaggaacta aaagatctcc
     5641 agcaacttca atttacagcg gaacaattaa acgaagaatg gcaaagatta caaattgaga
     5701 ctagagaaac aaattcagaa ttgaagattt tggaacaaag gtggcattgg cagaaaaatc
     5761 cgttaccgga agaattttta tatgaagaag aatggaagca tcttctggct gaagaaagaa
     5821 ggattgctga tctagaaagc cagatttctg tgcgtctcca aatagctaaa gaacagcaag
     5881 agatgattga agcagaagtg aatgacttgg aagagaccta tccagcatta ttacaaaatc
     5941 agtcagagat agcaccagtc tcttcatcta aaccgtatat gctgatggct ggcggagcaa
     6001 taatcattgc aggtatattt gtaccaatgc ccttgaaagt gatcctttta ctaattggtg
     6061 caggcatgat tggctatttc ttctacaaaa ggcaaccaaa aattaaaact aaaaatgatt
     6121 tctctgagat aaaaggaatg tggcaagaaa aactaggaca acttgatcta gccttagctc
     6181 aattagctga agcagaagaa aaacaaaaga gaactggaga agaaaaacag attcttcagc
     6241 aagaagtata tcgttttgcg gatcatcggc acttaggaaa gatggatacg ctcaaactca
     6301 tgaaagaata tggacaggaa gtcatagatt atcagaaatt atatggcgta tttgagcgga
     6361 aacataaaag agaacaggaa atacaagaaa aactacgaca aatcgaccaa aaatttgagt
     6421 ttgttaaaga atggttaccg atacaagata agacaatttc agaaaaaatg gtattgcttt
     6481 cccgttttat acaagagatg gaacagatga aatttgccag aagctatcag caaaatactt
     6541 tattgaagca agaaatcaat caattgaaaa agaaacaaaa agtatggata caagaataca
     6601 gcgaacaatt gactcaagca ggcttatctt atccttcgga agtacctgtc tttttacaaa
     6661 aagccaaaca agccgaacag cagatgaaaa gatacaatga attatcggaa atgcttactc
     6721 cactatttcc tgaagagctg acaaaagaga aattatctgt aagactgcgc caactgaaag
     6781 aaaagcaatc ccaactccag caagaagaaa aagaagcaag tgaaaatcgg caacgactcc
     6841 aacttcaagt agaagagttg caaagagacg gcacattgga cgaactttat caacaagcta
     6901 gccggcaaaa agcaaaatta caggaattac tggaggaatg gggagcactt gaaatcgccg
     6961 ggacattctt aggtgatctt tcaacagaga tgagtgaaag acaattaccc cagttgttaa
     7021 gagcagcagg tgattattta ggtattttaa caaaaggaaa ttatcagaag atattactga
     7081 aaaatgatac actgacttta acagacggat atacagaatt tcccatctat gagttatcta
     7141 caggtacgaa agatcagctg attatggcga tgagatttgc ttacctctca ttagaagcta
     7201 accgttcgat atgcccgatc atcattgatg atggctggct tcattatgat catcagcgaa
     7261 agtatcaatt agctgaactt ttagctaaat ttggagaaaa gtatcaaata atttgttttt
     7321 cttctgataa agaaatggta agctattacc atgagatgaa ccaaatcgta aaacaattag
     7381 aaggagcaaa taatgaaaaa aattcgtgat ttagtaatcg atgagcagtt tgaacaattt
     7441 gtcctgatca aaagtgcaga tgtaagaatc gctaaaaacg gaaaaaaatt tattgctttt
     7501 actttccaag atacttcagg tactatcgat ggtaaatact gggatgcttc ggaggaggaa
     7561 atcaataaat ttacagccgg cacagtcgtt tttctaaacg gtaaaagaga ggtttatcaa
     7621 ggaaaccctc aagtaaagat tcttcatatg cgcttaacaa gggtagatga gccaggagag
     7681 cctagtttat acatggaaag agcccctttg aagaaagaag aaatggaaga agaaatcaat
     7741 caattgttgt ttgagatcac caatgcgcat tggaatcgta ttgttcgcta tctacttaat
     7801 aaatatcaaa aacaattttt tgagtttccg gcagcaaaaa gaaaccatca tgcatttgcc
     7861 agtggattag cttatcatac tttgacaatg cttcacttag ggaaatcaat cgccgatgaa
     7921 tatcctgagc tgaatcgagc attgttgtat gctggtatca ttttgcatga tttaggaaag
     7981 gtgattgaac tttccggtcc tatgtcgaca gaatatacgc ttgcaggaaa tttgatcggt
     8041 catctggtat tgattgacga agaaataact aaagcctgtc ttgcgttgaa aatcagcgaa
     8101 gctgacgaag atgttcttgt attacgtcat atggttttag cacaccatgg cttattagaa
     8161 tatggctcac ctgtacgtcc aagaataatg gaagcagaaa tattacatca aatcgacaat
     8221 atcgatgctt cgatgcagat gatgttgact tcgattcgtc aaacagaacc aggaaaatac
     8281 accgatcgga ttttcggtat ggacaaccga agtttctatg taccaaaaga tttatagcaa
     8341 aaatttattt ttttcattca aaacaaaaaa agcgagacat aagtcttcgc tttttttgtt
     8401 ttcttttatt ttgatgaatc agttgtttct tcagttgaag attcttttgt tgaatcagtt
     8461 gattttgtgt cagaactctt tgttgaagct gtagtttcac ttgagtcttt cgtacttgat
     8521 gaagttgttg tgaatgcact caacacattt tcaaaggcat catctttgat ttttacattt
     8581 gcatctttta attcttcacc gattacttta gttgtgaatg cattgtcact tagtttagtt
     8641 tcagtagcga tatcttttag ttgatctttg tatttatcca tgtcattgcc tttattttgg
     8701 tttttcacca ttttgacaac gtagtattct gtcgcataag aagtaggatt tgttgtagta
     8761 ataacatcgg agatctcacc gtctttcaat ttgaaagctg cttcttttac ttctgcagga
     8821 atagttgttg tcgttgagtc gaatttaact ttaccgccat cttcttttgt ctctgtatca
     8881 gttgatttgt ctttggctaa tttagagaaa tcatctccat catccgctga ttttttcaca
     8941 tcttttgctt catcttcact tgataatttg atgatttgtg cttctacttc tggatggaaa
     9001 gatttccaag cagttttcaa atcgtcatct gtaatatcta cgtgagcttt caagccagct
     9061 tcaaaagcta aattgttacg aatatattct ttataagtat ctttcgtata tccagcagct
     9121 tctaattgtg attcaaaagt atcacctaat gatttcgctt gtttgtcgta ttctgcatcc
     9181 acttgcttat cggttacttt atcgccatat ttgttattga atactttata aatgatcatc
     9241 cgttgtacta aggattggtt cgatgattca agttttgctt catcatagaa atcagataca
     9301 gtgatcgttc cgcctttcat cgttgcgata tctttattag tatcgcctga acaagctgct
     9361 agagttaaaa cagccaacgc actagtagct gccaaaataa ttgatttttt tttcattaaa
     9421 atttgcactc ctgtaattag ttattatttt tatatattaa ctataccata tcccatttcc
     9481 tttaaaaacg ttgtcgtggt agtttcaaac aaaattcaaa atttcatcac attcctagga
     9541 aatacgcaaa cttttctcac ttatatagtt aaatctgttg agaaaataat cattattata
     9601 tttgttcagg atactctggc agatcagctt ttagtttttc tacttgttca ttgatttgtg
     9661 tgatacgtgg ttcggcttgg aatttaaaat cttcgatatc ttttttgatt gcttcttgga
     9721 aagcaggaat cacagattca gctgtttctt ttgtttcgat caatgcgctt ttaaaatgat
     9781 tcaggctgtt atttaaatca tttgtcagag ttgttgcttc ttccaattct tcgatcagtt
     9841 tctttcttgt ttcattcccg ctcctgggcg ccaataacag acctccgaca gtgccagcag
     9901 cagctccaaa taacagtcct ttgaaaaagt ttttaatcat cgcaggacac ctgctttacg
     9961 attgtttctt gaatagcttt tagttcttcc gatccataac tatcttgatg attgacaaag
    10021 tgaatagaaa aatcatcctc tttgctgtag cgagggacta aatggatatg tgaatgaaaa
    10081 acagattgat aggctagttc acggttgtta ttcaagatat taagaccttt catttctgga
    10141 aaagcttttt ccaatgcttg agcaatctta ggcacacgcg aaaaaacctc tcctgcaagt
    10201 tcgggatcat attcaaaaat atctgctaca tgacgtttag gcacaactaa tgtgtgtcct
    10261 ttcgttgctt gagagatgtc taaaaaagca tagaccttgt cgtcttcata tattttatag
    10321 cttgggattt cttgattgat gattttgcaa aaaatacagt tttccatata aaacatcctt
    10381 tctataaaga ataaatactt cagcttaact ttaccataag ttaaagctat tgtaaggaaa
    10441 aaaacaaaat accgtcattt tcttcgtggt ataataaata aaaataaatg gcggaggaaa
    10501 aagaatgagt ttaattatcg atcatgtcac aggggggtac ggacatctcc ctgtgttaaa
    10561 agatatcagt ttcgatgtac agcctggaga gatagtaggg ttgattggac tgaacggagc
    10621 agggaaaagt acgaccatca agcacattat cggtctgctg gaagcttcaa aaggaacgat
    10681 agaaatcaat catctatcct taaagaaaaa tacggaagaa tatcgcaaac aaattgggta
    10741 tattccagag actccttctt tatatgaaga attgacattg agagaacata tcgaagtcac
    10801 tgcgatggcc tacgatatac ctatggatat agcaatgaaa agagcagaaa agttgttgga
    10861 gacttttcgg ttatctaatc gtcttgactg gttcccagct aatttttcaa agggaatgaa
    10921 acagaaagtg atgatcttat gcgccttttt gatcgagcct tctttatata tcatcgacga
    10981 accattttta ggcttggatc cactagctat caatgcattg cttgaattga tggtagaaat
    11041 gaaaaaagaa ggagccggta ttttgatgtc tacgcatatt ttagctactg cagaaaaata
    11101 ttgtgatcgt tttgtggtct tacataacgg agagatacga gcagacggtt ctcttgagat
    11161 gttgagaaaa gaatttcagc tgccggattc ttctttggac gaaatctata ttgctttaac
    11221 aaaggagcag gcacatgagt agtttttttc aaaaacgttt agcaagacat caaaagaaaa
    11281 tgatgaaata tatgcgttat gtattgaacg atcattttgt tttagtttgt cttttcttga
    11341 ttggcggtgt gggattatat tattctaatt ggttgaaaac cctgcagcca ccgtttgcaa
    11401 ttggagggct cataatctct gtattttgga tgctctgttt attcgtgggt aagatggcta
    11461 cttttgcaga ggcagcagat attgtttttt tacttcctaa agaaaaagaa atgcaaacct
    11521 atttggaacg agcttttcgc tattcttgca tttttccgtt gatctttttg atattggcat
    11581 gcggattggc catgccatta gtggtggtta gtacggagca atccttttcg atgtttttcg
    11641 tctatctgat catattatgg agtttgaaat tcagtcatct gcaaatcaag agagcagaat
    11701 tgtttcgact agatcaacaa gttattcgtt attggaaatt aggctggttt ttttcttctc
    11761 tgatcttttt gtttctatca ttttattggc taatatgggc aggaatagca ggtgcattag
    11821 tacaggcaag tctatattac tggtttttgt ggaagcaaca agaaaaagga ttggattggg
    11881 agaaaatggt gcatgtagaa gaacataggt tgcatcagat ctatcaattt atcaatttat
    11941 tcacagatgt tcctgaaatt acggcaaaga tcaaaagaag acgctatttg gatcctatat
    12001 tgactcatat taaaagagaa tcaaaaaaca cttatcttta cttgtttgca agaagattgg
    12061 caagaggttc tgattttagt ggtctttatt tccgattagt aattataggt gggttattga
    12121 ttgccggagt agtcgacttt tattttagtc tgattatcgg tttcttattt ttatatttga
    12181 tcggtttcca actgttacct ttagcgaacc agttccgcta tatggttctc gttcagcttt
    12241 atccaatcca taagaatcag aagaagcagg cgattcagta tctactcaca tgggtattaa
    12301 ttatcgcgtc ggttattttt ggtttgattg ctgcagtcgt attagatggg cagcagcgat
    12361 gggttccgct agcggcttat ttgattgtta ctatgctctt tgttaaacta tatgtaccaa
    12421 ctcgtttgaa gaagatgtca gactgaaaac aaagctgtgt agggtaaaaa tgagaatgaa
    12481 aaatagagcg ttgacagtaa gaatgctgtt cgttctattt tttttgagaa aagctggttt
    12541 tttgaatggc aattaaatga aaaagaacgt atatgtgctt gacgtgtagt gttcaggcga
    12601 ctagaataat aagtactgca aaacaaagag taagaggacg ggagaatgca tgatggagtt
    12661 tcagttagac caagagtggc ggttacgtcc catcaaaggc gacacaggaa aaacatacat
    12721 aggcatgcgt gatgaggaca aagtctttat taaacgaaac acgactccga tgttggctgc
    12781 tctttcaagg gaaggaatta caccaaaact tgtttggaca aaacgaacgg ggaatggcga
    12841 tactttgaca gcacaagagt ggctggaagg tcgcttgctg actcctaatg agattggtca
    12901 aagaaacgac gtgattgatg ttttgtacca tttacatcat tcaaattctc ttaaaaccat
    12961 gctgacccgt atcggtggtc gtgtcatgac gccggagaag atgctgcaaa aatatgaaaa
    13021 agaattaccg gaagaactcc agaaaaatca atttctgcaa attgtccatc gttttcttcg
    13081 tgagcagatc ccgtcaatag ataaggaaaa cttatcagta gtacacggag atgtaaacca
    13141 tcgtaattgg ttagtatgta aaaattattt atatctagtc gattgggatt caatcatgtt
    13201 tgcagaccct gcggtggata ttgcaacgat tttaggaaat tatgtgccac tgtctagctg
    13261 gaatcagtgg ctgatcagtt atggcatacg cccgactgac gaaatgcttg aaaaatttca
    13321 ttggtatgcg gccatgaatt tgctccaaga aatcaccaga tattgcaaac gtgaagatca
    13381 tcgacggatg aacgaagaaa ttttacaatt aaaacgaatt ttcagtggct gaggtttggc
    13441 ccaaactctg cggagatttg gccagacctt tttaattaga ggaaaggaat ggagataaat
    13501 aatgcgtgtc cgaaatcgtc cgggagcagc ggaaatgtta gcggctcacc cgaattttgt
    13561 aattagtgat ccaacattgt ggaagggaaa atggaatgaa ttgtttgaaa acgatcatcc
    13621 aatccacatt gagattggta tggggaaagg ccagttcatc actggaatgg caaaagccca
    13681 tcctgaaatc aattatattg gagtagaaat gcaggtcagt gttgtttcga ttgcactaga
    13741 caagttgatc gaacaaccgc ttcctaacct gaaacttcta catgtggatg gatcagcgtt
    13801 gacagagtat ttcgctgaca gcgaagtaga tcaaatctat ctgaattttt ccgatccatg
    13861 gccaaaaaaa cgtcatgaaa aacggcgatt gacatataag acttttcttg ctgtagatga
    13921 acagattctt cgtccaaatg gagaaatcca tttcaaaaca gataatcaag gattgtttga
    13981 gtattctctg gcaagttttt ctcaatatgg catgatcctc aaacaggtat ggttagattt
    14041 acatcaaagt caatttgaag gcaatattat gacggagtat gaagaaaaat tttcttcaaa
    14101 aggtcaacgt atttatcgag tagaagctca atttcaagat aagaccaaaa agaaaagtct
    14161 gtgaaactaa ctttctgctt tcataaaaac attaatatcc ataaataaat ctcagaaaat
    14221 gaacattaca gatgtaatgt attttctggg atttatttga tttaaaatgt atttatttca
    14281 tcggtttttc cacaaggtgg aatcaattgg aagcgagaaa atgaaaataa tcctttgtcg
    14341 gtgatcttca attcgggtat gacagataat gttaagaaag aaagtgtcaa aaaaggatca
    14401 aaggttttag ctgctcctag ttgatgtgct ttagttatta attcattcaa ctgttcatag
    14461 acgatcgaaa aaggttcctg tgtcatgatt ccgcctatag gaagagaaac acaagctatt
    14521 tcctgctgtt tgttgacaac ggtcatcccg ccaccttttt gaatcaaaag attagcagca
    14581 tacagcatat cctcgtcgtt tgtcccaacg acaactagat tatgcgagtc atgggcaatc
    14641 gttgtcgcta tcgctccttg tttcagctga aatcctttca ctatgcctaa tccaatgttt
    14701 cccgtttgat ggtgacgctc gatcaccgca atctttaatt ggtctttttc gattgaagga
    14761 atgaatcgtt cgccttgtcg gtccactttt tctaccaaat ctttagtcac tagactattc
    14821 gggataattt cgatcacatg ggcttgtgaa gaagaaagaa gcagctctaa cgaatgtgga
    14881 tttaatggtt gagcgtttag ttttggcaga caacacgtat atgaatttga atgatcagca
    14941 aataaatggt cttggagttg cccagattga acaactaatg ttcccttata gaaaacttca
    15001 tgaattggca gcttggtaag atcgtctgtt aagaagaaat cagcttgata cccaggggca
    15061 atcgcaccta gtgttttcaa atcaaaacat tctgctgcat tcaatgttgc catctgaatg
    15121 gcttgtaatg gatcgattcc aagtgcaata gcttgacgga cgatatgatc gattgatcct
    15181 tcttttacta aatcattgat cagtttatca tcggtgacaa agaagcatct tcttgaattt
    15241 tcaggtgtga tcgccggtag tagcgatgcc agatcttttg caacggtccc ttctcgaacc
    15301 attaaataca taccaagatc caaacgttct ttagcttctt ttgcagttgt agcttcatga
    15361 tccgtccgaa tacctgctgc aggataaaca tttaactctt ccatgccgat accagctgca
    15421 tgcccgtcaa tttttttctt atgctggtgc atcaattgga tcttttctag gatgtctgtt
    15481 tcattatttg ctactgcttg atagttcatg acttcagcta agcctaaaac ttcgggtttt
    15541 tgtaaaaaag gggctaattt ttttgcattt aaatttgcac cattggtctc aaaaggggtt
    15601 acaggtacac aagaaggtag cataaaaaag atattcatga gtgtttgtct tgcatcttct
    15661 atcataaact caagacctgt actcccagca atgtttccta tttcatgggg atctgtcact
    15721 acagtagtaa ctccatgaag caaggatact tttgaaaatt cgcttggtga taaaagtgag
    15781 ctttcgatat gcacatggcc atcgatgaat cctggcacta gatattgccc attagcatta
    15841 tgtacgcttt ttccttcgta ttcaccgatt cctacgatca ttccttcgca gattgctaca
    15901 tctttttcca tgatttcttt cgtaaagacg ttgacgatct tagcatgctt gatcactagg
    15961 tcagcaggct tttttttact tgctacttcg atatatttcc ttaatgttgt ttccaatgga
    16021 gtgcctccca agataaattt ttataagtat aacataaatt cggcttttgc caatattttt
    16081 ttgtaaattc attttatttg aaacaaaaag atcaaaaaga cgtatgtttt ttgtggaggg
    16141 gagaataatg gaaataaaac gattatcgga tcaaatgata agtgaaagtt ttgccaaaca
    16201 tgcccaagac gtatatatat tgccagcttc agaagtattt tacatctatt ttcgctatgg
    16261 aaaaaaacga acattctgga agaaagtctc ttcggcagat ggacagcaac tgatctcaag
    16321 atttaagtat ttgggacaga tggatatcgg agaaaaaaga aaagctcagt taggtgcatt
    16381 tacttattct ttagaaaaac aggaagttcg tctgcggttg tcttctgtag gagattacct
    16441 tcaacgagag agtttagtga ttcggctatt atacggtact tttgaaacac cccatcgttg
    16501 ctttagttct cgagattttg caactatttt acagaaaacc gtcagacgtg gattatacct
    16561 tttcagcggg acagtcggat caggcaagac ttctctaatg tatcggttgg ctttggaaga
    16621 aaaattgcaa gtgattacta tcgaagatcc tgtagagata gaagaaccgt tttttctgca
    16681 gctacagatc aatccgaaaa tagcacagac ttatgataca cttttgaaac tcgcattacg
    16741 gcatcgtcca gacttgctaa tcattggcga gataagagat gaatcaactg cacatgctgc
    16801 ggtaagagca gcactaacag ggcaccgggt ttttgcaacc gtacatgcac gcaatcttga
    16861 aggtacattg gtcagaatcc atgaactgac aaagaaaaaa gaagagctat ctgaatgttt
    16921 agctggtgtc atttatcaag aacttttgga agatgccaat caccatcctt ctgttttatg
    16981 gagttatcaa tttatggagg aagaatcagt aaaaaaatca tgggaggaaa gttatgaaga
    17041 agctcaggaa aatatttggc agtcagaact attctaaagg attaacaaga agccagcaaa
    17101 accagtttgc acaaaccatg ggagaactgc ttttatctgg ttttagtctg caagaatccc
    17161 tttatttatt actcacgatc cgttcgtttc cacctgttat tttggcaaat atccaacatc
    17221 agctaaagga agggcaagcg tttagtgagg tactttcgca aataggatat ggagcagagc
    17281 aactggtaca gatcgagtta gctgaacgtc atgggaatct gccgacaact ttgttggaaa
    17341 ttgccaaaca gtttcggctt gttgaaggat ttcgcaatga tttgaaaaag ctgcttgcat
    17401 atccgatgct attgctcacc tttttgctag gtatcttgtt tagtatgcgg ttgttcgttt
    17461 tgcctcagct tattggttca ggaatggtca tgcaagagca ttggggtatt cagctgatac
    17521 aagtctttca ttggtatgtt ctactattca tcctagcggt tactctattg gctttactag
    17581 taaggtatcg tctgatgaag ttgtctcctg ttgatcagag tgaatggata agcagacaat
    17641 tattgattgg accgatatat tccttgtatc aatcggctta tttgtcacta gagattggaa
    17701 agctttttca tgaaggtttc gaattaaggc aaatcatagt ctgcttaaag gaaacgaaac
    17761 aagaaagtct gattcagcat cttgcctcta agttgattca aggattggag gcaggtatgt
    17821 cattggctga gcagttccag aattatcgtt ttctatcaga agaattcagt aaaattgttt
    17881 tacagggaga ggcaaaggga agtttaggta aagaactgat tttttacagc gaatggacaa
    17941 gaaaacagct tttcaaacgt cttcatcact ggatgcattt tattcagcca atcatttttt
    18001 tgggcgtagc ggctttgatt ttattagttt atgccgctgt tttattacct gtttacggaa
    18061 gcatagagga ggttttgcca tgaaaaagag aaaaatatct tattatagcg gttttacatt
    18121 gatcgaaatg ctaatcgtct tactgatcat cagtgtatta gtgttgttgt tcgtccctaa
    18181 tttgtcgcgt tataggaatc atgtagacca agaaagtcgt gaagcgatca tccagttggt
    18241 agacacacaa aaagagctct atgcgcttca aaataatgga agagttccta ctgtggagga
    18301 attgttgaat gaaggctata tcaaacgaga acatgcagaa atctatcagc ggccttgaag
    18361 gtaaggtttt agcatcagga tatacgttgt ttgaatcact cttactgatt ttattactta
    18421 caacgatcct ttcttttcca gttctcgcat tttctgcatg gaagaatgaa ttggcagtct
    18481 atcaattttt cggccaattt gaaaaacgaa tctattctac gcaaaaaatc gctattgtga
    18541 atcagatcca aacaggcttt tattggaata gtgaagagaa tcaaatcatt tttcatgttc
    18601 cgaatccaga taaaataaat tgggaagtat tagatattcc tgatgagatt accttgaaac
    18661 gccatgcttc tattactttt gctgctagaa ccgggaatga aagttcacta aaagcttatc
    18721 aattttattg ggaagaaaaa gagcggacga ttacgtatca attccagata gggagtggtc
    18781 gctatacaaa aagaattgaa taaaacagga tttgttttgt tggagtccct tacagcgctt
    18841 atgatcgctt ctgtctgtgt ctacttagtg agctttggcc agctgttttt gcttcaggca
    18901 gaaaagaaaa gtggggagga actgctattt attcgaatga tttatgaaga tatcaagacg
    18961 cagcggatgt atcacaaaag accagagcga agggaagaaa tgatcccata cgatatccaa
    19021 attgtcgata ataatggagt aatcgaaaaa gtgtttgtca atatggagaa agaagcgatc
    19081 agtattgaaa aaacaagtaa ttccagcatt tacgctgatt gaatgtctaa ttggactgat
    19141 gatattgagc agtctattgc tcacattcaa tcttttgatt cagcaaacca tccaagtaag
    19201 tgaatttttg cagcgtaaag atcaaaaaga gtggctaata tttttagcac aattagaaga
    19261 agaactcaag agcagtcaca atgtctctgt gaaaaatcag caactgcact acacgatcga
    19321 ggataaacag tatatcatcg aaagatatca ggcaatgata cgtaaaagaa gaacttccgg
    19381 cggacatcaa cctatgttaa caagtatcac tgagcttcaa ttattggaaa aagaacacac
    19441 aatttctttt tatgtacact ttgaaaatgg agaagatgga tatgccatat ggacaaaaaa
    19501 cgatcagtag aaaatgttat aagggctcta ttttattttc tgtcttgctt ttatttcttt
    19561 tatgtacgtt tttattgatg gcagcattag aaagctaccg tttgtcaatg gatcttaata
    19621 tacgaatcaa aaattattat atagcaaaaa taatgaagca gatggcttta tcggaacagt
    19681 ctacgcttgc cgaaagttcg gaaggagttt tttactacac aactggatct gtaacatatc
    19741 agtgggtaca acagtcccta ttcttagagg tggaagtctc accgtttatc tttcggttca
    19801 cagaagaagt aaaaaatgat acagatacta gtacagaatg atggaatgtt cgtgaaaacg
    19861 ccctcttttc tgttataaca ctgtttttaa taaaaaaatg tttgccgttt cacaaataaa
    19921 aaacagtgaa aacgattgaa attttgtcaa ttgttcgata gaattaaaca ggtgaaaaaa
    19981 ttgttataat atatctagaa atgaggtgca ccatgttacc agaaaaaatg gaacaagggt
    20041 ttaatcaaaa cctggaagct attcagcttt tacagaatgc attgggaact tcctttttag
    20101 aagcctatgt cgaaaatgca gaaaatttaa tagatgatta tcaagtacgt gtggtggatg
    20161 gagtgccgac aaaagagaca acgcaacgga tcacagctct ttatgaagag ttgaagaaac
    20221 tttctttcga gccagaagaa tggcgcagac tgtctcaatt acttttattg aagggcagcc
    20281 agacagaaca tttgcaggcg aaccatcaac tcactcctga tagtatcggt tttttatttg
    20341 tttttcttat tgaacaacta tacacaaata aaaaagcacc ggttaagatt ctggatattg
    20401 cggcaggaat gggcaacttg ttattaacag ttcttttaaa cctatcgaat gcggggtacc
    20461 aaacagaagg aattggtgtg gatattgatg atacattgtt ggctgtcgca gcttctacaa
    20521 gtgatttgac acaagcaaat gttcaatact ttcatcaaga cgggttgcag gaattattaa
    20581 tagatcctgt cgattttgca atcagcgatt tgccaatcgg ttattatccg aatgacgaaa
    20641 aagcaaaaga gtttttaaca agtaccgaag aaggccatag ttatgcacat catttattgc
    20701 tagagcaatc aatgaaatat gtgaagccgg atggttttgg tttgtttttg atgccgagtg
    20761 gatttttaga aacggatcaa agtgaagaaa tcaaaaaatg gtttaaagaa gaaggttatc
    20821 ttcaaggaat gattcaattg ccagatgaat tgttccgtaa taaacaatcg caaaaaagta
    20881 ttttgatctt acagaaaaaa ggaccacaag cacaacaagt aaaagaagtc ttgcttgtga
    20941 aacttgcttc attaaaagaa ccagagaagg tgacggaatt tttcaacgag tttaaaaact
    21001 ggaaatcatc tgctctgtaa tcattcaacg agaaggagag tcatatgtct aaaacaattg
    21061 caatcaatgc tgggagttca agtttgaaat ggcaattata tcaaatgcca gaagaaaccg
    21121 tagttgcgaa agggatcgtc gaacgtatcg gtttacaaga ttctattttt accatcaagt
    21181 acggcgataa tcaaaaattc gaacaaatca tggatatcga aaaccacgaa gtagcagtaa
    21241 aaatgctgtt agatcagttg attgaactgc aaattttaag ctcttatgat gaaatcaccg
    21301 gagtagggca ccgtgttgtt cacggaggag aaacttatgg ggattcagta gtgatcgatg
    21361 agaaagtaat ggaccgcatt gctgaattag cagaatttgc tcctttgcat aatcctgcaa
    21421 acttaatggg gatcaaagcg tttaaaaaga ttttacctga cattttgagt gtggctgtat
    21481 tcgatacatc tttccactca acaatgccaa agcaaaatta tttatatagc ttaccggtgt
    21541 cttattatga agatttcaaa gtaagaaaat atggcttcca tggcacaagt catcgttatg
    21601 tgtcggaacg tgcggcaaaa ttattgaaca aaccgatcga agaactaaaa atcatcactt
    21661 gccacttagg aaatggtgta tcgatcacag cagtcgatgg aggtaaatcc atggatactt
    21721 ctatgggatt cacaccatta gctggggtga caatgggtac acgttcaggt gatatcgatc
    21781 cagctgttct cccatattta atggaaaaat tagagattga tattgatgaa atgatcaatg
    21841 ttttgaacaa gaaatctggt ctgctaggcc taacaggatt gtctagtgat atgcgtgatc
    21901 ttgaaaagaa ctatgaaaaa gaacatattc gtttagctta tgacattttc gtggaccgca
    21961 tccgaaaata catcggcggt tatgtgacaa cgatgaatgg tgttgatgca attgtcttta
    22021 ctgctggtat cggcgagaat gacggacatg tacgtagtga aatcatcaaa ggtatgtctt
    22081 ggtttggttg tgaaatcgat ccagcgttga atgaattgcg tggtgaagaa gtagatatct
    22141 ctacaaaaga atcaaaagta agagtattag tgatcccaac agatgaggaa ctaatgattg
    22201 cccgtgatgt ggagcgtctg cgcggctaag ccaaagcatt cgttaggata aaagatgaaa
    22261 cgcatgcaag agggaattct tgcatgtgtt ttttttgcgg agaagaccgc aaaatggtaa
    22321 tttaacttca atttcccttc ttttttacag gatcttctta gggaattttg cttggaaaag
    22381 tggtcaaaag ggaaaagact ggcattttat ttattgattt tcaggaaaat cccaatattt
    22441 tattttcatt catctttata agggacatta catatgtaaa aagaaaaaac atttttagat
    22501 cagcctcgtg ggctgtcgac tttattcttc gctttatgtt tagctagaaa accgatccga
    22561 aacattcatt gatcgaataa taaaaacttg attttccacc tgtactttgc ctgaatcgtg
    22621 aaagcacagg tatgaaaatc aagttttttg ttgcgaaaaa acgtcctagt caatcagttg
    22681 gccgagcggc tggcggctga tctgctcctg atcaataacg agtgcttctt ggaagtgttg
    22741 ctgcaaaaag tagcggtcat ggctgactgc taggagcgtt ccttgatagt cttgcagcat
    22801 ttcttcaatt tcttctcgag catagctgtc gaggtgattg gttggttcat ccaaaatcag
    22861 gacgttgatt ttttctaaaa agagtttcag caacgccaaa cggatttttt caccaccgga
    22921 taaatctttg atccgtttac ccacatcgct ttgataaaag ccacttttgg ctaattgccg
    22981 tcgagcgact tcttcgtttc cagtcaatgt gatggcgtaa gccagcaaac ggctatcggg
    23041 atcggcaaac tgcagctgct gcggcaaata accgatttgc aagctactac ctaatttgat
    23101 ggtgccaccg taaggctttt cctcaccgag taataatttg agtaaggttg attttcctgc
    23161 accattgtcg ccgatgatgg ctaaatgatc tccacgatag actgcaaaac tgctgtcttt
    23221 gaatagtact tgatcagcgt actgctggtg caagttttga atgatgaaaa cttctttgcc
    23281 agattttcca ccattagaca agctttgcag ccgatttttc gggggaggtg gcggtttgac
    23341 gagtgtcagt ttcgccaggc ggcgttccag ttctttggct tttttgaaga aggactcatt
    23401 gtctccttca tgtgcccact gacgaaaacg acgaatcatg actttcaacc gctggacttc
    23461 tttttgctgc agttcgtaat tttttgtcag ctcagccaag cgagcttctt tcaagactcg
    23521 aaaacggctg tagtttccgg gatactcaat caatgcacct tcttccagtt cccatgtttt
    23581 tgtggtgact tgatcgagaa attgccgatc atgagaaacg accagaaccg atttttttgt
    23641 attctgtaaa tagttttcca accagcgaat actgttaaga tctaaatgat tggtcggttc
    23701 atcgagtaaa agtaaatcgg catcggctgc taaaattttg gctaaagcga ctttaactcg
    23761 ttcgcctccg ctaagttctg ttaaccgtgc ttcctgacag tgtggaatcc ctaagccttg
    23821 caagatcgct gcaatccggt cttctaatac gtaaccgcct agttcttcaa agcgatgctg
    23881 cagctcaccg taacgggcca gacacttctc taagtttgta tctggttctg tcatcaatgc
    23941 ttcgtagcga ctcatttgct gtttgaggtc ctgtatctca gcggcagaat ggagcaagta
    24001 gtggttgacg gtcgcttcgg aaactgtaag ttcttgttcg accgtcccga tctttaagcc
    24061 ttttttgcga ctgataacac cgctgtcgac accttctctg ccggtcaaga tttgtaaaaa
    24121 ggttgatttg ccagttccgt tgatatcgac taaggcaatt ttttcaccag cttcaatttg
    24181 acaggataaa tcctcaaata atggattccc gccgattgtt tttttaatag aaattgcttg
    24241 tatgatcatg atctctctcc ttgagaccac tcaaaaaggc catggttatc cctggcctgt
    24301 gttatttaaa ctcagattat catgagaaaa ataccttgag atggccattg attttacgtg
    24361 aattcaactg ttttggacaa acttgtcccg tcaacagcaa ctgtaaaacc aattgcatgg
    24421 ctaaatgcga tatggtatcg ttggtaattt tcattacttg ttattttagt catgcgcatc
    24481 atagtatttt ccctccttcg tgtagttgtc ttttccagtg tacctgaaag tgtcagaaag
    24541 ttcaactagc gctgacattt acttaatatt tatcgatgtg taattctgtc agagagcttt
    24601 ttttatctga ctgagggtaa tgtctcgtgg accacatcat tgtattctga gctaaaccag
    24661 cgttcaggcg aaatcatatg gtgtttgata aaaaactttg tctacatgac ttccttttgc
    24721 catttgctta ataccgttgc attaaacagc tcttctcttt ggaaatctca ccaacatcgc
    24781 gttcaggagt atcgttgtgg ttgcaatggc aagcggtagc ctttcatcaa ttcttgaatg
    24841 atttaccgaa tgtagcactt gccttctcgc ggtacccgaa cccgaaataa aattttagtt
    24901 tggcgataaa atcggtatcc gccatcgata cttcatattt tttcgtatca gattcatcgg
    24961 aaacttccac attgacgaac caataagctt tggcatgctt aggtcgtttt aaaccacacc
    25021 agggcgacta atcgccctgg tgttcacttt ttttatttta cttgaactca ttgactgttt
    25081 tccctttatt ttaaacatta taaagaccga tgagtgcttg ccgcgtcttt tgtttttatc
    25141 acggtttttt gtgcaaaaaa acctgccgat agttggcagg tcgtctacgt gttaagcttt
    25201 ggtgtggtct ttagctggca attggttttc taaatcagtt cggacaacaa gcacatcgca
    25261 agctgcgttt ctaataacat attcagaaac agaaccaata aataaacgtt ctacagcatt
    25321 caatcctgtg gctcctaaca taatcaaatc tacttgatta tcttctggga tttctctggc
    25381 aataatttgt tttggtgagc catattctac gacagaggtt acattattca agccagcttt
    25441 cttcgcattg ctttcataat ctgccaatgt ttgctttgcc atttcagtag cttgttctgc
    25501 aagcattcca tcaaaggatg aaacggtttg gaaagaacgt gtgtcaatga catgtgctaa
    25561 tagaagttct ccgttatttc ggatagctac attgaccgcc tttttaaaag ctaattcagc
    25621 ttcatcagaa ccatctacgg caaccatgat ttttttgtat tgttgtaaca taagcattca
    25681 ctcctcaagt caatcgtatt gtactctttt attgtagaac actttaggga aaaagagaat
    25741 tatttactgc tttggttcag caattttttt tgcaaatcgg atactgaagg tggcagaaac
    25801 aataattacg atcagtaaaa agaccaaggc aataaagcgt tgattgaaaa aagctgttaa
    25861 aatacccatt acgcctaaaa cagcatatgc agcaccgtaa aattgtagga atccttgatt
    25921 tttatcattt ttgttgatca atacaaaaaa gatatcctgt ttattaaata aataatagct
    25981 aacgaataat aatactaaag tggcgataat cattaaaatc ctaatcatga acaaatcctc
    26041 caaaataagc aacaaataga aaaagcaatt tgccaagagc aaattgcttt attctttaaa
    26101 agatggtaat ctccgttaat gccgttgttt gtcctgtagt attgatcccg cgaattcaag
    26161 caggtgggtc ccatggtcgt agcttcgaac taccaaatga taaggcatgt taccagcagt
    26221 gacacaagac gggtcccaag atatcaataa aaatgttcgg tcaacacaca aagaggacaa
    26281 ttcgaacatc acagatttcc cacctctata gtagcatatg aatttaaaaa gagcaatgag
    26341 agagctcgca gaaacagaac cgttttcttg tctagttttt tggaggatgc tttttccact
    26401 cttttatacg gtaatactgt tgacccagag cctgttcata tttccctgta tcttttggct
    26461 gataatactg agcattcttc agtttatctg gtaaatattg ctgattgacc caagcctgat
    26521 caaaatgatg aggatactga taacccacgc cacggttgag tgatttcgca cctttgtaat
    26581 ggctatctct caaatgatca ggaacatctc cagctttgcc ctctcgaata tcagcaagtg
    26641 ctgcatctaa tgccatgtag gcagaattgg attttggcga caagcaaaga tcgacgacga
    26701 cgtctgctag tgggatacgt gcctcaggca atcccaattt ttcagcagct aatacagcgt
    26761 tcactgtacg agccgcagca gcaggattac ctaaacctat atcttcataa ccgataacca
    26821 tcagacgccg gcaaatactt gccagatcac ctgcctcaac taaacgggcg agataatgaa
    26881 gtgcagcatc cacatcactt ccacgaatcg atttttgaaa agccgaaata acgtcataat
    26941 gagcatctcc atttttgtca tgggtaagag cttttctttg gacacattct tcaatgatcg
    27001 acagtgtaag atgaatcctt ccttctttat ctgaaggagt cgatagtgta gccagttcca
    27061 gcccattcaa tgcactccgc aaatcgccgt tcgttgcacg cgaaaggtga agaagagcat
    27121 cttcgtctaa ctggattgct tgttgtccaa ggccgcgttc tttatccctt aaagcatgtt
    27181 cgactgccaa ctggatgtct tgttctgtta atggtttgac ttcaaagatc tgtgtacggc
    27241 tgcggatcgc tggattgatg gtaatataag gattctcagt agtcgctccg atcaagatga
    27301 ttcggccact ttctaaatga ggaagcagaa aatcctgttt tgttttatct aatcggtgta
    27361 cttcatccaa taagaggatc actgtaccac tcatttttgc ttcttccgct acaacttgta
    27421 gatccttttt tgaatctgta gcggcgttta gcatgcgaaa tgcataatta gtagagccgg
    27481 cgatcgcact tgcgatactc gtttttccag taccaggagg tccatataga atcatggaag
    27541 agagcatgcg agcatcgacc atgcggcgta tgatttttcc ttccccgact agatgctgct
    27601 gaccaacgac ttcatcgatc gttcgaggtc tcatgcgata agccaatggc tgttgcatag
    27661 gattttcctc cttcttgttt ttacgtccct taattatatc atatttagca agatgcctag
    27721 aaagaacagc cttctgttgt tttgttttca tagcttatcc aactaatatt gtgtatgata
    27781 aagtaggtga gaaaaatgac ggataaacaa aagatagatg aatttttttg gaataaaacg
    27841 acgccagaag tggcacggga tttattgggg atgtatttgg agcatgacac accagaagga
    27901 agattagctg gatatattgt agacgcggaa gcatacctag gtccagaaga cgaggccgcc
    27961 catagctatg gtttaagaag aacaccgcgt gtgcgggcaa tgtatgaaaa accaggtacg
    28021 atctaccttt acacgatgca tactcatcgg attttgaaca tcattactca gccagaaggg
    28081 attcctcaag gagtaatgat acgtgcaatc gaacccgccg caatgattga tcaaatgagt
    28141 aaaaatcgtg gcggaaaaac tggtccagat atcagcaacg gtccgggaaa attagtggaa
    28201 gcattagcaa ttcctcaaga actttatggt cagtctatag cagatagttc gttacgctta
    28261 gtttttgaga aaaaaaagac accaaaaaag atcaatgctt tgcctagaat cggtatccca
    28321 aacaaaggtg tatggacaga aaagccgttg cgttttgttg tgtcgggaaa tccttatatc
    28381 tctttacaaa gaaaaaacca aatcgagaaa gattggggtt ggaggaaaga aaatgaaaaa
    28441 gaaggatcta ttaacatatt tagatgaaat aattgaaaaa caagtgacag actatgatgt
    28501 agctttagat tgggatacaa aaaatcatac gatcgagatc gttgttcgct tgtttgcaga
    28561 aaataaagca caatttgaga ttgatgatgc agaagggatc gtttcgcaag aaccaattat
    28621 cgaatttgaa gacggcgtac ttctttttaa tcctcagaaa tctgtttttg atgaacgcaa
    28681 ttatctagca gtaatccctt atgaaggaaa aaaagggttg gcaaaatcag tagctgattc
    28741 tttagtggaa tatctaaatg aagtattagc tcaagggcaa agcgatttgc ttgatttttt
    28801 ggatgaagac gatgacgaag ctgtattcga gcttcactgg gataatcaac aactagcaga
    28861 attagtagaa gtaaaacagc aaaatgaagc agatacttat ttaccgtacc caagttatta
    28921 aggagtgaca gatatgaaat ggaatgaagt aaaagtagaa actgaaagtg aagcagtcga
    28981 agcagttgct aatattttga tggaggcagg ggcgagtggt gtcgcgattg aagattcttt
    29041 agatgttgaa cattttcagt cggatcaata tggtgagatt ttagataaaa atgattttac
    29101 atcgctgaaa gaaggcgcac tagtcatggc ttattttccg gaaacgactt tcttaccgga
    29161 aatcttgcct tttatcaaag atcgtatcac acaattacct gagtttggtc tagctattgg
    29221 aaaaaataaa gtaacagtta gtgaagtaga ggaaagcaac tgggcaacag cctggaagaa
    29281 atactatcat ccggtgcgta ttacgcgaat gctgacggtt gcgccaagct gggaaaacta
    29341 ccagacagaa gatcctctag aaaaaattat cacactagat cctggtatgg catttggcac
    29401 aggcactcat cccacgactt ccttgacatt gacagcacta gaaacagtct tgcgtggtgg
    29461 tgaaacattg ctagatgtgg gaactgggtc gggcgtgcta agtattgcag caaaatatct
    29521 tggtgcaaaa gatgtctatg cctttgattt agacgaagta gccgttcgtt cggcgaaaga
    29581 aaatatggat atgaatgaaa tagccaaaga tgtccatgta tcagctaacg atcttttgac
    29641 aggaatcgag attgaaagtg atgtaatcgt tgcgaacatt ttagcagata tcatcttatt
    29701 gatgatccca gatgcttggc gtttactgaa gcaaacaggc acgctgatcg tttcaggaat
    29761 cattgaagag aagaagcaaa tggttctgga tgccatgtta gaacaagggt ttatcgtgga
    29821 tcaaattttc cagcaaaaag actggtatgc aattattttg aaaaaaccag aggtggaata
    29881 gatgcagcgg tattttttaa aagaatcata tgaatcaaaa gactattttg ttttagaagg
    29941 ggaaaactat caccacattg tacgggttat gaggatggaa cccgaggatg aagttttttt
    30001 ggcattcaat gatcaaatag ccattgttgc agaaatcacg gagattactg aagaggctgt
    30061 ttatctgaaa gaaattaaaa aagaagaaaa tcaaaaggaa ctgccaagtt cgattaccat
    30121 agcgagtggc tatcctaaag gggaaaagct ggattggatc gttcagaaat ccactgaatt
    30181 aggagcgcaa gcttttcttg gtttcccggc atcagcctct gtcgttaaat gggatgcaaa
    30241 aaaacgtatg aaacgacaac aacggcttga aaagattgct caagaggcag cggaacaatc
    30301 ccatcggcag atcgtgccat cagtagagtt attggaaacg aaacaagatt tatttgagcg
    30361 gctagatgat tatgacgtgg tcttggttgc ttatgaggaa tcagcaaagc aaggcgaaaa
    30421 atcaaatgca gccaaaattc ttagtgaaac aaaaccagat agtcgaatct tagctgtctt
    30481 tggaccagaa ggtggattat cccgcgatga agtcgattat tttgtagaac acaaagggat
    30541 actttgtgga ttaggaccaa gaatccttcg tacagaaaca gcacctcttt atttattaag
    30601 tgctgctagc tatcaatggg aattaatgaa tccttctttc agctaacgtt cttcattaat
    30661 aaaagagtat cgacagaaag aattgaaaaa atagtcaaca atttgtataa tagaaacaat
    30721 tatttataca atggatacga aaaatcgttt gaaaaagctt ttaaaaagcg aacatttccg
    30781 taaaaaaacg tataactgca cggattttgg gacaattatc aaacagatat ttgtcccgat
    30841 tcccgtttta ccgaaaaaag aaagggggat cgattcctgt gacaaaagaa gaaattttaa
    30901 cgggacccgg tgtcataaag ctcgttagtc aatacatggg tcccgaacat gtagcatttg
    30961 ttgagaaagc atgcgaatac gcgactgctg cacatgacgg acagtttaga aaatcaggcg
    31021 aaccctacat tattcatcct atccaagtcg caggtatatt agcagattta aaaatggatc
    31081 cccatacagt ggctacaggc ttcttacatg atgttgttga agatacagaa atcactttag
    31141 aagatctgag agaagaattt ggcgatgacg ttgctatgtt agtggacggc gtaaccaaat
    31201 tagggaaaat aaaatataaa tcccacgaag agcagctagc agaaaaccac cgaaagatgc
    31261 tgctagcaat ggctcaagat ttacgagtta tcatggtcaa attagctgat agactacata
    31321 acatgcgtac gttgaagcac ctgcgagaag ataagcaaag aaggatcgct caggaaactt
    31381 tagaaatcta tgcgcctctt gcacatcgtc tagggatcag ccggatcaaa tgggaattag
    31441 aagatacagc acttcgttat ctaaatccaa aacagtatta ccgtatcgtc cacttgatgc
    31501 agacgaagag agaagaacgt gaaaaatacg taagtggtac tgttgaagat attcgaatag
    31561 cgacagaaga gctggggatt tttgcagaaa tctatggacg gccaaaacac atttattcaa
    31621 tctatcgtaa aatgaaagat cagaaaaagc agttcaacga aatttatgac ttgctagcta
    31681 ttcgagtgat cgtagattca atcaaggatt gttatgctgt attaggagca atccacacaa
    31741 aatggaaacc aatgcccggc agatttaagg attatattgc catgccaaaa gctaatatgt
    31801 accaatcttt gcatactacc gtgatcggtc cggcaggaaa tccggtagaa attcaaatcc
    31861 gaacacagga aatgcatgaa atcgctgaat tcggggttgc tgcacactgg gcctataaag
    31921 aaggaaaaaa tgaaaaagta gaaccagatg gtatgacgaa acaattaagc tggttccatg
    31981 agatactcga acttcaagac gaaagctatg atgcttctga atttatggaa ggcgtaaaag
    32041 gagatatctt tagtgataaa gtctacgtct tcacaccaaa aggagacgtt actgagttac
    32101 caaaaggatc cggaccattg gactttgcat acagtatcca tacagatatc ggtaacaaaa
    32161 ccactggtgc aaaagtaaat ggcaaaatgg tgcagcttga ttacaaattg aaaaacggag
    32221 atatcattga gatcatgact tctccaaatt catttggccc aagtcgcgac tggttgaaat
    32281 tagttgctac tagcaaagca agaaataaga tcaaacgttt cttcaaagcc caagatcgag
    32341 aagaaaatgt gatcaaaggc cacgaatccg tggtcaaatg tattacagat ctaggattta
    32401 cgcctaaaga tattttgacg aagaacaaac tgcaagaagc actcgatcgt tttaattatc
    32461 aaacagaaga tgatctctat gcagctgtag ggtatggaga agttagcccc ttgacgatgg
    32521 ccaatcgtct gactgaaaaa gaacgtaaag aacaaaaaat cgagcagcaa aagcaagaag
    32581 cagaagaaat catgaatcag ccgaaaaaag aacctgacaa aatgaaagta cgtcatgaag
    32641 gtggcgttgt cattcaaggg gtagaaaact tactaattcg tatcagtcgc tgctgtaatc
    32701 cgattcctgg tgatgatatc gttggttata tcactaaagg cagagggata tccattcatc
    32761 gtcgagattg tccgaatgtt cagcctgaca aaccaaatgt agcagaacgt ttgattgaag
    32821 tcgaatggga agatacatcg aatacacgaa aagagtatga tgcagatttg gaaatttacg
    32881 gctataatcg ttcaggctta ttgaatgatg tacttcaaac agtcaatgcg ctaacgaaaa
    32941 atctcaacag cgttgaagca cggacgaata aagataaaat ggcgacgatc catttgacgg
    33001 ttggtatcca gaatttatcc cacctaaaga gtatcgtgga taaaatcaaa gcagtacctg
    33061 atgtctacag tgtacgccgg acgaatggat aggaggacag atatgagagc agttgttcaa
    33121 cgggtaagta aagctgctgt gtcagtcgat cagcaagaaa ttggaaaaat tggctacggc
    33181 ctacttgttt tattaggcat tcatgaaacc gatacgcaaa aagatgtgga ctatctggta
    33241 aaaaaaattg cacaaatgcg tatttttgaa gatgaacaag gaaaaatgaa tattagtgta
    33301 gaagatgtgg gaggacaaat tttaagtatc tctcaattca cactgtttgc ggatacgaaa
    33361 aaaggaaatc gaccaagctt tgtggaagcc gcacgaccag aaacagctat tcctttatac
    33421 gaatcattca atgaaggaat cagaagtcgc ggcatcacag tcgctaccgg acaattcgga
    33481 gcagatatgt cggtttctct cgtcaatgat ggacctgtaa cgatcattat tgattctaaa
    33541 aacaaataaa gtaatctggt tttcactagt aaaaactatg aaacacgtcg cttaagttag
    33601 actgataaac tctaacttaa gtggcttttt tgtctgcaaa aagcgcttct tttgtcagca
    33661 gtcggaaaaa aagttaagct taaattatac aagttatttt ttataggagg ttgctgaaga
    33721 tgaaagaagt accagtagta gatatcaaaa tcacagattt aaaaaaattg tatcaaaaag
    33781 gatacaacat gttggaggag ttacgccatg aagcggacgc tcccgtcgtc aaagcaaaga
    33841 ttttcaataa agaagctatc acgatctacg gatcatcagc agcaaaagta ttttatgatc
    33901 caaggaattt caaacgaaaa ggtgctatgc caaaattagt attgaagaca ctttttggtc
    33961 aaggtggagt acagactttg gatggagctg ctcatcacca tcggaaaaat atttttatgg
    34021 atctaatgac accggaaaga atggaagatt atcatcgtat cttagataag aatctgacac
    34081 aagcacttga agcacagcat ggtcaatttg aactatttga tttaagtaag atggttttct
    34141 ttacatctat ttgcgagtgg gcaggtatca atttatcagc gatttctaaa gatgaggtcg
    34201 aaaaattagc agaatatcaa atctctatga tcagcggaac gtttacttct ccgattgatc
    34261 acataaaagg tgtcgagaat cgcaaaaaat cagaaaaatg ggcgcaaggc ttgattgaag
    34321 aagctcgaca aaatcctgtg gctggaaaag aaaatgtggc attatatgct tttgccaatg
    34381 ccacagattt agatggtcag cttttacctc tggaagtggc agctgttgaa ttattgaaca
    34441 tcatccgtcc aacggttgct ctaactgtgt gggcggcatt gatgggacat gcacttttca
    34501 gtagaccaga tttgtatcag caattaaaaa atgacttttc gacgttacaa gatccgttta
    34561 ttcaagaaat gagacgctat tatcctttct tcccaatgct acctgcgatt tcgctgaaag
    34621 aagtagaagt agatggttat cgtataccag aaggcagttg ggttatttta gatttatatg
    34681 gaacagacca tgatgaacga acagttgaag cgccagattc ctttatgatc aaacggtatg
    34741 tagggaaagc aaaagatatt tcctataaag aagaatatga gatgattgca caaggcgggg
    34801 gaaattttcg acaaatgcat cgctgtgcag gagaatggat cactcttcat agtctacgtg
    34861 tgttttccga tcagctagta aataagtttg agttctctgt acctgaacaa gattggacga
    34921 ttccattcaa tcagtttccg acttatccga atagccgagc gctactttac aagaattaga
    34981 ttagcataaa cttatcgaaa aaaaggaaga tcaaacagtg ctgatcttcc ttttttcata
    35041 ttactataaa ttgataaggt caattgatca aaatccacga aatgcttcat caatggtttg
    35101 ataatcttca ttggataaat gcaaatctaa agcttggaca ttgtcagata cttgctctgg
    35161 acgcttagct ccaggtataa ctacagacac tcgtggattc ttcatgtacc aagctaaaac
    35221 aagttgcgca ggagtggctt ggtatctttt agcaatgggg cgaagtacat cgacagcggt
    35281 taaaatttct ctgaaacgtt ctccttggaa atctggattt ctttttcgag gatctccttc
    35341 accaaattgt ttttcctcac caagttcata ttttcctgtc agcaaaccgg aagcaagagg
    35401 aaaataggga acaaaactga ttttgttttt ttcaagataa gggaataatt ctttttcagc
    35461 ctgccgatgg atcaaactgt atttatcttc tactacatcg acatatccat ctgcattcgc
    35521 ttctttcaat tgttctaacg tgaagtttga cacaccgact gctcggattt ttcctgcttc
    35581 ttttaattcg tgcaatgtag caactgattc attttttggt gtgctttcat ccggaaagtg
    35641 gatataaaaa atgtcgatgt aatcagtttg cagacgtttt agtgcatctt ccaccgattg
    35701 tttgagaaac tcaggagaat tatcaaatgt tcgcccttta tttggtacat gagcagcttt
    35761 tgtagcgatg acgacacgtg agcgatcata ttctttttcc ttcagtacct cgccaatcag
    35821 ttcttcggaa cggccgtttc cgtaagcata agcggtatcg atcaaattga tcccgctatt
    35881 caaagctgta cgaactactt gttttcctgt ctcatcttcc aaaccgtcaa aaagattatg
    35941 tcctccaacg gcatttgctc ctaatccaag ctgttctgcg tagacttgtg aatgtccgat
    36001 tcttatttct gccatagctg attcctcctt tttgtctagt ctaaaacaaa ataccaaaaa
    36061 gctgaactaa aacgtttttg tgaggggaaa tagtcagata tctcttgact ttgagactgg
    36121 tttttagtag gttataagta atatcgaatg tcaatgaaag agcgagtaag tttttgaaac
    36181 tttacaagag aggatcatcg aggctgaaaa tgatctaaag tttcattagc ctaaagacac
    36241 tctggagatg gacgagtgaa aagccgtccc gcgtacgagc gttattcgta agaggaaaga
    36301 gaaatctttc aaaattaggt ggtaccgtgt atgaatttca ttcacccttg ttcatgatga
    36361 acaagggtga tttttttatt tacatagcta agaaagggag agaaaactat gagtttccaa
    36421 agaccaaaag gaacaaatga tattttgcca ggcgaatcag aaaaatggca gtttgtcgaa
    36481 gaaacagcac gtttattatt cagagattat cagtataatg aaatgcgtac accgattttt
    36541 gaacactatg aagtgatatc ccgaagtgta ggggatacaa cggatatcgt ttcaaaagag
    36601 atgtatgatt tttatgacaa aggggaacgc catgtgacgc ttcgaccaga aggtacagca
    36661 ccaatcgttc gttcttttgt tgagcataaa ttgttcggcc cagagtatgc gaaaccgttc
    36721 aaaacttttt atatgggacc aatgtttcgc tacgaacgcc ctcaaaaagg acggttacgt
    36781 caattccatc aaatcggagt agaagcattt ggatcagaaa accctgcaac tgatgtggag
    36841 agcatggtga tggcacttga tttcttcaaa cagttgggga tcacgcaaat ccggctagtc
    36901 atcaattcat taggtgataa agaaacgcgt caagcctatc gtcaagcatt gatcgactat
    36961 cttttacctt ttgaatcaga gctgagtgaa gactctaaac gccgccttca tgaaaatcca
    37021 ttacgcgtat tggacagtaa agacaaacga gatcaaaaat ttgtggcaga agctccatct
    37081 atcttagatt atctaagcga gcctgctcaa aaacattttg ataccgttgt ttccatgctg
    37141 gatgcattgg ctgttcctta tgaaatcgac agtaatatgg ttcgcggact agattattac
    37201 acacatacga tttttgaaat catgagtgac gcaccaaaaa tgggggcaca agcaacgatt
    37261 tgcgcaggcg gacgttatga caatctagtg gaagaactag gtggtccttc aacacctggc
    37321 ttcggatttg caatgggtat cgagcgatta ctcttgacta tggaggcaga ggaagtagtt
    37381 atccctgcat ttaatgagtt ggatgcttac gtggtcgcac tcggagacga aacaaatatc
    37441 gaagcattga aagtggtcca agcgattcga aactttggtt tttcagctga ccgcgacttc
    37501 atgaaccgta aagcaaaagc gcaatttaag acagcagata aagcgaatgc caaattagta
    37561 ttggtgatcg gtgaagatga gcttgctagt caaacgatta atgtcaaagc aatggcaagc
    37621 cgaaaagaaa aaagttttcc attagaagag atatatgaac attttgatga agtctatgat
    37681 gaaatgactt tattcgagta ggaggaaaaa aataaatggc acaaagaacg atctattgcg
    37741 gattagtttc cgaacaacaa ttagaacaaa cagttacatt aaaaggatgg gtacaaaaaa
    37801 gacgagacct tgggggagta atctttatcg acctacgtga tcgtgaagga atcgttcaaa
    37861 tcgtctttaa tcctgaaatc aacaaagaag catgggaaat cgcggataaa tgtcgcagtg
    37921 aatacgtgat cgaagtgact ggggaagtta gaagacgcgg agaaaatgca gtaaatccta
    37981 aaatggccac tggtgaattc gaagtgattg caagtgaaat cgtgattttg aatcaagcaa
    38041 aaaccccgcc atttcaaatt gaagatgatc aagcaatcag tgacgaaata cgtttgaaat
    38101 atcgttattt ggatctgcgt cgtccaaaaa tgactaaaaa catcaaatta agaaatgcga
    38161 caacaaaagc gattcgtcat tatttagatg ataatgactt tctcgatatc gaaacacctt
    38221 acctagggaa atctacacca gaaggcgcaa gagactacct agttccttca cgtgtccatg
    38281 ctggacattt ctatgcgttg ccgcaatctc ctcaattgtt taaacaattg ctgatgaatg
    38341 ctggattcga ccgttattat caaatcgttc gttgtttccg cgatgaagat ttacgtggag
    38401 accgtcagcc tgaatttacg caagtagata ttgaaacaac gttcttaaca gcagaagaaa
    38461 tccaaactta cacagaggga ttgattgcga aggtaatgaa agaagttcgc ggtatcgaag
    38521 tcacattgcc atttcctcgt atgacctatg atgaagcaat ggctcgctac ggcagcgaca
    38581 aaccggatac acgttttgat atggaattga tcgatctaag cgatacagtc aaagaagtag
    38641 aattcaaagt cttccaaatg gctcttgaaa acggcggtgt cgtgaaagca ttgaatgcta
    38701 aaggcgctgc agatcgctac tcacgaaaag acatggatca gttaggtcaa tatgtaggac
    38761 aattcggagc taaggggctt gcatggctga aagtagaaga agacggattg aaaggtccaa
    38821 ttgctaaatt catgggagaa gcaacagaag caatcattaa agcaacagat gcaaaaccag
    38881 gtgacttgct gatgtttggc gcagataaaa gtgaaatcgt cgctgctgca ttaggtgcta
    38941 tccggacacg tttaggaaaa gaattaggac taattgatga atcgaaattc aatttcttat
    39001 gggtaactga ttggccacaa tttgaatttt cagaagaaga aggtcgatat gtctctgctc
    39061 accatccatt cacgatgcca aaagaagaag atatcccgtt attggctgaa gaccctgcaa
    39121 aagtctatgc agaagcttat gatatcgtct tgaacggtta tgaattaggc ggaggttcta
    39181 tccgtattca tactcgtgag cttcaagaaa aagtactaga aactctaggc tttacaaaag
    39241 aacaaatgga agaacaattt ggtttcttgt taacagcttt agattacgga ttccctccac
    39301 atggcggtat tgcattagga ttggaccgtt ttgcaatgtt attagccgga gaagataata
    39361 ttcgtgaagt cattgccttt cctaaaaacg gaaaagcagc agatgtgatg agtgatgcac
    39421 caagtatggt ttcgccttta caattgttcg aattgaatat tgatgtaacg aatatagaag
    39481 aatagccgat aaaaaggtaa aaaggatttg tcgaatacgc ttcttttgac aaccttgatt
    39541 taattgaaaa acactttata atgaagccat aacatataaa gcggggcttt tccatgaaaa
    39601 aaacattcga aaattgggct tatcatgatt acacgaccaa gctttcggta tctatcgttt
    39661 atgcgttgat cgcatcagtc gcattgaatt tcttttatca gccaggacat atttattcaa
    39721 gtggtattac tggtctagct cagattttga cgactttgtc aaaatcactg ataggattcg
    39781 aagtaccggt atcaacaact ttgtatttac tgaatatacc tttgtttgta ttggcgtggt
    39841 taaaaatcgg gaagaagttt acaattttca cttttatcac agtcactttg acctcccttt
    39901 gtatccatat tttaccggaa acagtcctat ctaccgatcc aatcatctgt gcgttgtttg
    39961 gggggctgtg atgggactag gtattgggct agcgttgaga aacggcctat catcgggcgg
    40021 tctagatatc gtgagtatca cgattcgtaa aaaaacagga aagtcgattg gttctatttc
    40081 gatctacttc aatgcattga tcgtttttgt tgccggaatt ctatttggct ggcaatatat
    40141 gttttacagt acgttgtcta ttttcgtcag tggaaaagta actgatgtcg tttatactaa
    40201 acaaaagaag atgcaggtaa tgatcgtaac gaaacatcca gaaaaagtag taaatgaaat
    40261 ccaaaactgt atgcgtagag ggatcacgat catcaatgaa gcagaaggtg cttataagca
    40321 tgataagcag acagtattga tgacgatcgt tacacgatac gaactgcctc tacttagaga
    40381 agcaatgaaa gcttctgatc cacaagcttt tgtaagtatt gcagaaaacg tccagatact
    40441 tggtcgtttc tatgaggatg gcatttagat cgagtttgac aaaaattcat gaaatactgc
    40501 tgtagacaga cgttcgtttg tctgcagttt ttttctacca tagtagaatc agcaaagaaa
    40561 aactgctttt ttatttgaaa ttttcttaaa gttgaaaaaa agcatgttta tttttttaaa
    40621 tttatgggat gatagaaaaa aatagtagag aaaggacacg attctacttg ctattttcac
    40681 ttaatacccg tatattacga atgaaaaaag tttatgaaaa gttggaatat tgaatggaaa
    40741 aacaaaaaag gttaaaaaac agccgctaca tcactgggtt tgatgggctc cgttcattag
    40801 ctgtggtggg tgttatcttc tatcaccttc tgccaactag tatgaaaggc ggctatttgg
    40861 gcgttccgat ttttttcgtc gtatctggtt atttgatcac ggatctgctg cggcaggaat
    40921 gggaacaaaa tggaaaaatc aatatttggc aattttatgt cagaagaatg aagagactct
    40981 atccggcact tgtttttatg cttgtgacag cttcggctta tatcactttg tttcagcgag
    41041 ggctgttaaa caatcttcga ggagttgtgg tcagcagctt actgtatgtc aacaactggt
    41101 ggcaaatcaa taacggttta tcttatttcg atcgatttgc taatgagtca ccgtttacgc
    41161 atatctggtc tttagcagta gaaggtcaaa actatttggt ttggccgatc ttgtttgtat
    41221 tgttgatggt atttgttcgt aaaagaaaat ggatctttta tactgtgtta ggtacatcct
    41281 tcctctccgc catcttgatg atgatcttgt atacaccagg aggcgatcca acccgtgttt
    41341 attacggtac cgacactcgt ctgttttcta tatggttggg aagtgcgctt gcgtttgttt
    41401 ggccaagtac gcgtttaaag aaaaacatcc cgaaacaagc gaaaaatatt ctgaatatcg
    41461 ctggaggaat ctcgcttttg gcactgattc tttgcttctt ctttttagat gatcattaca
    41521 atttcatcta ttacggagga atgctcctag tgagtctgtt atgcgtagtg ctagttgcag
    41581 tgacagcaca ccccggagca agtttgaatc gatggctgac aaatccagta tttacttgga
    41641 ttggcaaacg aagctatggc atttatctgt atcagtttcc agttatgatt ttttatgaag
    41701 caaaggtgac tgatattgcc gatcatgttt tgttgcatac tttagtggaa atcacattga
    41761 ttctaatcat cagtgaactt tcttatcgat atgtggagcg tccgctagca cgttttgatt
    41821 atggtcgtac attccaagtg atgaaaggat ggttcagcaa accgatcatc agtaagagca
    41881 aaccatgggt tgttccaggc acattacttg tagcaattgc actcgttggt ttcgttacgg
    41941 caccgaaaaa cgctgtaaca gccgatcaaa aacaactgca ggcaaaaatc gctgaaagta
    42001 aaaaattagc agaagaaacc agaaacaatt cagaaaacac cactagttcg gttgaccaag
    42061 cgatccttga taagtacgag ttgtccaagg cacaagggaa taaggcacaa aaattagaac
    42121 taacggcatt tggtgattca gttatgttgg atgctgcaac ggatctacaa gaactttatc
    42181 caaaagtagt ggtagatgga gatgtcggac gacaattata tgccagtgta ccgtacattg
    42241 aaaaattaaa ggaacaagat ttgttgaaag acactgttct gattggacta ggaacgaatg
    42301 gctcattctc ggaagctcag tttgatagtg tgatggatgc aatcggtgat cgaaaagtat
    42361 actgggtcaa tgtccgagta ccgacgaagc gctggcaaaa tgacgtgaat gcgatgcttg
    42421 aacagatgac gaaaaaatac gacaatatga cgctgattga ttggtacagc tacagtaatg
    42481 accatagtga ctggttttat gatgatcaag tccatccaaa tcctgaaggg atgacacatt
    42541 acattcactt agtcagcgaa gccttgctag gaggaagttc aagtacgaac agcagtgaga
    42601 caaacgtgac agaatgatag ctacaaaaaa cttcaggcaa aaacatttgt ttttgcctga
    42661 agttttttta tatttaccgt gtttttaaca ttcatataga ttcaggaagc aaaacggttg
    42721 agtgtgtctg taaataacgt tctttttgtt ttcgggtgag ttttttgaat gcagcttctg
    42781 cttttgttgc ttcgcttctt gaagaaaata cttctgcatg gatcatctta acaggtcttc
    42841 ttttttgaag acgagtatat ttagcaccag taccattgtt atgttcctct aatcgtctag
    42901 aaagatcggt tgtatatcca ccataaaatg tctgatcttt acagaataat acgtaaaaat
    42961 aatgttcatt tgccatagag catctcacga atctctgcag tgtattctcc atccatattg
    43021 taagtgatca atggtggagc gactttgaat ccgtctaatt tgccttcttt gattccttca
    43081 actaacaaaa tatttgcctc ttttccaggt tttgggtaaa caaattggat ccttttaggt
    43141 gcaattcgat agtcttccat gacctgaatg atttctaaga accgatcagg acgatggacc
    43201 atagtaaagc gtccatttgt ttttagtagc ttacttgaaa tatcgatcac ttcttctaac
    43261 gtcgtatgaa tctcatgacg ggcgattgcc aggtgtttat tagggttcgt tttatttgtt
    43321 ggcagccctt tgaaataagg tggattacag acgactaagt cacaagaatt atgtcgaacg
    43381 acagataagc tgtctttcag atcgatggtg tggacagtca tttgttcatt taaatggtta
    43441 agcgcgatac tacgtcttgc catgtctgct agacgaggtt gaagttcgat agcgtcgata
    43501 gccgcctggg ttcgtttact taaaaaaagt ccaactgcgc catttcctgc acaaaggtca
    43561 acaatcttcc cttttttagg tacacgggaa aagtgagcaa gcagcacagc atctaaagaa
    43621 aaagaaaaca ctttggaact ttggataatc ttgatatctt ctgcgtataa ttggtcgatt
    43681 cgttcgtctt cgtgaagcat ggattccctc caaaatgaaa aaaatagtcg tctgtattat
    43741 actattttta ttaaaaagaa ccaaggtggc ttgtcaaacc tctatttttt atgcatacta
    43801 tgaccataag aagggaaagg aagagactat gttttatcga tttatgcgcg gggtagttcg
    43861 agtactatta gccttagtta atggaaatgc ccgctatgag aataaagaag ttttaccgca
    43921 agatgagaat tacatactag ttgctcccca tcgtacttgg tgggacccat tatatctggc
    43981 ggtagctgca gcacccaagg aattcagctt tatggcaaaa gaagaactgt tcaaaaatcc
    44041 agttctgcga tttatattaa ctcgatccaa tgcttttcca gttaaaagag aaaatccagg
    44101 accaagcgtc attaaaacac ctgtaaagat tttgaaatcg acaaatttga gtttgatcat
    44161 gtttcccagt gggacaagac atgctacaga acttaaaggc ggaatggcat tgatttccag
    44221 aatgtctaaa gcgaaaatcg tgccagctgt atatcaaggt ccgttgacac ttggggatct
    44281 gttcaagcgt cagagagtga cagtgcgttt cggcgaacca atcgacttat cagatatcaa
    44341 gaaaacggat aaagaaggta tggcggagat cgaacgacga acacaagcag cttttgacaa
    44401 attggacaaa gaagtgaacc ctgatttcaa atatgaagtc aaaaaaaaat aaaatgataa
    44461 gtttatgcgt gggcggtctt ttgaatctac tgtcctgttc agttcagact ttttcaatga
    44521 aaacgaggga gagacgtcaa gtctgccctc tttttgcgtt ccaatatgta aaatagaaga
    44581 caggtgtcat gtaatggatc aaagtgttat aataattaga aggacatttt ttgaggtgat
    44641 tgaatgcgtt ttttacatac agccgattgg catatcggaa aaaaactaca aggttatgac
    44701 ttgattgaag accagcgtca tgtactatct tctatactag atatcgcgaa agaagaaaaa
    44761 gtagaggcaa tcgtgattgc cggagactta tatgatagga gtgtcccagc tgttgatgcc
    44821 gtagaattat acaatgaatt gatggtcgat tggaatttaa aggaaaagat cccgattttt
    44881 gctatatcag gaaatcacga tagcagtacg agactttcag cgggtacgcc atggttcagt
    44941 tattcgaact attttttgcg aacacagcta gcagaagcat ttgaaccagt cgagtttaaa
    45001 aacgtacaat ttttctttct tccttacttt gaaccaattg cagcgagatt atattttgaa
    45061 gatgacaata tccgaacgat caagcaagcg gtagccaagg tgatagataa aatgcaggag
    45121 ctatttgatt cagaaaaaaa acaagtattg attagccatt tttttgtaat gggaagtgta
    45181 aaatctgatt cggaaacgaa aatcgaagta ggcgggttgg attcgatccc tggtgaacta
    45241 cttacaccgt ttgattatgt agcactagga catttgcata atcaagctgc tttgagacag
    45301 gataatgctc gctatagcgg ttccccattg aaattctctt tatctgagat caaccaaaaa
    45361 aaaggcgtat ggatcgttga cctgacaaca ggcctggagt tgaattttag ggagatcgaa
    45421 cccctgcatg atattgaaga aataacagcg agtttcaaag aattgctgga tcctgccttt
    45481 tatcagtccg tcaatcggga gaattacatc tatatctatc tcactgatag aacgatcatc
    45541 ccgaatatga tgaaccagct tcggcaaata tatcctcgta ttttggggat tgaacgggca
    45601 aacggtcgag cagaacaaac aaagcgcaaa cgaaaagtac tagaaatgaa agatcctcaa
    45661 aaattagcag aagcattttt tagtgaaatg acggaagagc agctaactgc aaaccaagaa
    45721 aaatggctcg gagaaacgtt gcaagaaatc atggaaatgg aatgatcaag aatgaaaccg
    45781 aaaaaattac aacttaaaaa ttttggtcct tttatcaatg agaccgtaga tttttcacgt
    45841 ttgacagaag caccgctttt tttgatcagc gggaagacag gagctggcaa aacgacgatt
    45901 tttgatggga tcaccttcgc actttttggt gaaacttccg gaagacttcg ctctggaaaa
    45961 gagatgcgtt ctttgtttgc tacaccagac gaagaaacaa gcgtgacttt ttcttttgaa
    46021 catcagcaga tgagctatga aatcgaacga aaaccagaac aagttttagc aaaacgaaaa
    46081 ggaaacggaa caaaaaaaca ggcagccaaa gtaacgttga ccatttttga tgggaaagga
    46141 aaagaaataa aacaaatcac aaaacgtaca gaagcagatc agttgatcag agaactgatg
    46201 aatctcgatg cgaaacagtt ttctcaaatc gttttactac cccaaggaga attccgtaat
    46261 tttttggttt catcaagcag tgataaagag actgttttac gtcatttatt cggtactcaa
    46321 tttttccaac agttcaatga gcgattaaaa gaaaaagcaa aacaacagca acaaaaattg
    46381 gatcatttag agcaagagct gcatcttctg caaaaaaggt ttgttttagt caaagaagag
    46441 gaaccagcag cagggtttga agccgtttta gcccaatgga caaaccatca ggcaatactc
    46501 gaaaaacaaa tcacggaaga aaaagaagct ctaacggaac ttcaggaaaa acaaaagcaa
    46561 ctagaaaatt cctactatcg ttttgaaagc ttgaaaaaaa gttatgaaga aaaaacgata
    46621 ttaataaaca agcaaaagga attagctgct caaaaagacg aaatagagga aaagaaacga
    46681 tggattgggt attatgaatt tacgcaacga ttagtcgaac caatccagcg cctacaagaa
    46741 ataaaagttg aaaaaataga actgtcacaa aaaagtagtg agcaaacaaa gaaactagcg
    46801 atgactacgc agaattatga agagtggcaa aaaaatgaag caaaccgcgt acgccgacaa
    46861 acaaaaatca aagaagctgc tcaaaaactg caaaacaaac agatgatggt gccaattgtc
    46921 gaatccttac gtagtaaaaa acaagacgca gaacaattaa caatttcatt ggataaagaa
    46981 gaattgaatg ccgagaagct gtatcaaaat ctcgctatct atcaggaaag aactcgaatt
    47041 cttcaagaaa atttactgga tcaggaaaaa ctgcaagaag atcgtcttgc ttttggacag
    47101 ttaaaatatc tagatgaaaa gcagcaagaa atagaaaaac aaaaagcaaa agacactggg
    47161 tcgctcgcac agataagaga agaactcaaa aaagcaactg aagaaataac aaaaaatagt
    47221 cagctattga aagaacagac agaacaagca gaacagctga aaagccaatg ggcaaagcag
    47281 caaattgctc gtcttcagct ttttttgcgt ccaggggagc catgtcttgt ttgtggatca
    47341 acggaacacc ccatacaaca attaaaacac gaacagccaa ataaggaaga tatcttaaaa
    47401 gtagaacaac aactggcaga agctgagtct gctgtagaaa gaactcagca aatcattgcc
    47461 aaaagtgaag cacatgaaaa taatcttcaa caacaattat cagatttaga acaaaaaatc
    47521 atggcaacga atatacaatt ggctgaacaa gaagaacact tgcaacaggc tttgaaaaag
    47581 gcatttcctg agataacaga acaaaaaaca aaaacaactc ttgtagtaat cgaacaatct
    47641 ttagatagac gaaaaagcaa gatggccaat gaaagaatag agctggaaga aataagacaa
    47701 caagcagaaa agctcgaaca acaattagct gtctacaaac agcagcagac agaaaaaaat
    47761 aatcagctcc agcaactgca aggagaaatc aaaagtttag aacaacaatt gcaagatacg
    47821 gatagtaact gtttagaaga ggagatacga cgacttgaaa aacagttaga agaagactcg
    47881 aatcaactag ctgttgagca gaaagcagga gtgcaattgt tgcaagatct tcatacgctt
    47941 cggacacagc ttgatcttca acatgaacaa atagaaaaaa tagatgaaaa aatagaaaag
    48001 gaacaagaaa gaatagctaa aaagttagaa agccagtcgt atttttcaac aattgaagaa
    48061 atctgtgcat ttgcacaaca gcaagcacaa tataaagaaa accaacgtac agttacttct
    48121 tatcatgaag agcaattagt gacagctgac cggctgcgtc aattagaaaa tatccagcta
    48181 actgagacat tcccagatac agaagagatc agccaacagt tagaagaaat aagacgtatg
    48241 atcgaaataa agcaagaaaa gatatacggt ttacaagaac aaaaaattag taatcaaaaa
    48301 atctacgatg aatgtctttc gatttatcac acaagccaga cgaaattaga tgagcttacg
    48361 caattacagc agctttcgca aacattcaac ggagaaaatc ctaagaagac tagtttggaa
    48421 cggtatgtgc ttcaggttta tcttcaagaa gtgttacaag tagcaaatga tcatctccaa
    48481 cgtttaacga aaaatcgtta tcaatttgag ctagcggata ccattggcag ttatcgaggg
    48541 aaaaccggat tagaaatcaa tatctatgat gatcaggcag gtatgtctcg aagtgcgcat
    48601 accctgtctg gcggtgagag ttttattgca gcactttctt tagcattagc tcttgccgat
    48661 gtgatccaga cacaagctgg tggtgtagca atcgatgcat tgtttattga tgaaggattt
    48721 gggtcattag atgaagaagc tttggaaatg gcaatggaag ctttagaaac gatcgaaagt
    48781 gaaggaagaa tgatcggcat cattagtcat gtaagagaat tgaaagaacg agtattacaa
    48841 caaataagaa ttgatacaga aggtagcgga caaagcaaaa tacgctatca acttggataa
    48901 aaagggagat tggtaactat gaaatggagt attccagaaa aagtcattgc acgtggacgt
    48961 caatacttgc aagaaggccg agtgttatcg gttgtccctg accaagaaga aaacgtgtgg
    49021 catgcagaag tcttgggcag tgaattgtat atggttgatt tggatggtac agcaaaagag
    49081 atagattact gccaatgtcc ctattgggag gaacataaat attgtaagca tacagttgct
    49141 gtggaacttt atttacggtc gaaaaatcta tctcgtatca tgaaaaaaga ccagccaatg
    49201 ctagaaccgg taaaagcaac aagtgaggga gaaatcattg caaaaggttt ttctcgtcta
    49261 aaaacacaaa acaatcctca agcggtgcaa cctttgatta ttgaatacca agtagaaacg
    49321 attgagacta atcagtatca cccagagctt tctattttgg cactttactt gcgtatcggt
    49381 tcattggcat caccgaaaaa aacatatatc gtaaaaaaca tctatgattt tttacaagct
    49441 tacacagaaa aagaaactta cactgttaat aaacagtacc agtttcgtct agataaacgg
    49501 gcgtttcaaa aagaagatca aaaaatactc acgcatcttg caggaagtgc tcagacgcat
    49561 caattattag gtacgaacgg cttacaagtc aaaggaaaac tagataaaag atatctgctt
    49621 ctcccagtag aacaagcccg cttcctgtta gagcagatga atcagactac acgtttgcat
    49681 ctgcagataa atgaacaaaa cgatcaagga atacaatttt ctgatcaaaa caaaccttta
    49741 tcattcaaag tggaaaaatt tggagaagat tatttgctgc aagcattgaa tgattttgat
    49801 tttttcttta tgcattatca gtggggagtt atccaaggga cattttattc tctgacgaaa
    49861 tatcaacaaa cggtttatca aacttggaaa caattgattc gccgattgga gaaaccagaa
    49921 gtagtgttca agaaaaaaga attaccgtct ttgtttaaag agattatccc tctgctgtca
    49981 gagatcggcg acgtagctat tgcccctgca gtagaagagg aagtagcaga tatccccgtc
    50041 gaatttattt tctttttccg aaaagcaaaa ggaaaaatcc aagctcgagt cgatttcgtc
    50101 tatggtgaag tcatctattc gactgacccg aagcatgagg tctcgacaga acaggctttt
    50161 caggtgttac gtgacacagt agaagagaaa cgagtacttg atctattcag aatgtatcgc
    50221 taccagaaaa atgaaactgg ttatgaacgt ccattgccaa gcggggaaga actatatgcc
    50281 tttttccgca cagaactatc tgtcttccgt cagtttggtg aagtcagact tggaagaaaa
    50341 ttgcgagagt tgtatttaga tgcgcataaa catcaaccga aaatcgaagt aaccgagacc
    50401 ggttcatggc tcgatattcg attcgatgtg acagggatag aggagcaaga aatcgatcat
    50461 gtcctgcaaa gcttgctgag aaatgatgct ttttatacat tagaaaatgg tcaggttctt
    50521 tcttttgatt cagaagaatt tcaacaaaca agccaagtgt tgcaacaatt acgggaatcg
    50581 atacgtacag aagaaggaac gatccatgta cctaaaaatc aaggattgat cattcaaaat
    50641 caattggaaa aaagcaatgc tacttttagt gaatcgttcc aaacgatggt acaagatctc
    50701 attcatccag aaagatacca ggcacagctt cccaaaggat tgaatgcaac gatgagagat
    50761 tatcaaaagc aaggttttcg atggttgaag atgttaggtc attatcagtt tggcggtatt
    50821 ttagccgatg aaatgggact tgggaaaaca ctacagacaa ttgccttttt actttcggaa
    50881 aaagaagaac ggaaaagctt ctctgctttg atcgtagcgc ctgcgagctt aatttacaac
    50941 tggcaggcag aggtgagaaa attcgcccct tctttaagta tccaagtaat caatgggaat
    51001 aaaaaagaac gtgaagaatt actggctaaa gacacagata tccgagtgac ttcttatgcc
    51061 agcttaagac aggatctagc taattatcag tcgcaaaaaa ttgattattt gattcttgat
    51121 gaggcacaaa tggtcaaaaa tagcagtacg aaaactgcac aagcattgag agaattagct
    51181 gtacctcagc ggtttgcact gagtgggaca ccaatcgaga ataatttaga agaattgtgg
    51241 tcattgtttg caacgattat gccaggattt ttcccgacta aaacaaaatt cagggaactt
    51301 gccgcagaag aaattgcgca aatgattcga ccgtttattt tacggcgaga taaacaaact
    51361 gtgttgaaag acttgccgga aaaaacagag atgaatctat attctgcact tacagaagaa
    51421 caaaaaactg tttatttagc ttacttgcgt caaatgcgtg aagaaatcac atcaatggat
    51481 tctgaagcat tcaagaaaaa ccggatcggg atacttgctg gtctgacaag actgcgacaa
    51541 atttgctgcg atcctcgtct gtttattgaa gattaccaag gcggttccgg caagctcgaa
    51601 caagtaaaag atctactggt agctgccaaa gagaacaaac gaagagtttt gctgttttct
    51661 caatttacag ggatgctgac tatcttgcaa gaagagttag ccgagctagg gatctcgaca
    51721 ttttatttaa gaggcagtac gaaaccacaa gatcggttaa gtatggtaga cgcatttaat
    51781 gctggcgaaa aagatgtttt tctgatttcc ttaaaggctg ggggaactgg attgaatctc
    51841 acgggagcag atacggtcat cttgtatgac ctttggtgga atcctgccat tgaagaacaa
    51901 gctgctggac gtgcacatcg gatcggacaa aaaaatgtgg tagaagtgtg gcgtatgatc
    51961 tctgaaggaa cgattgagga acgcatggat tcactccagc aagaaaaacg cgaactgttc
    52021 caaaaagtga tccaaggtaa tgaagaacag ttgacgaaac tgactgaaga agacatccgc
    52081 atgattttaa gtgtaggaga aatcgaggag taagagactc gaaaaagcgt tcagtaatgg
    52141 gtaagaaagc ctttctaaag aagcgattgt ttatttgatt taaaaaatag caaggaggct
    52201 gggattccat tttatcccag cttccttgct atcatttttt tatttctatt tttaaaggtt
    52261 atacaaagat tgtcttcatt gtctctaaca gctgatcaaa agaagaaatc tcgatatcgc
    52321 aaattggagt ctgttcaatt ttgcgttctc gatggttgaa ccaaagcgct tgccagttag
    52381 cactttttgc acctagaaca tcattgtcgt aattatcgcc aacgtataag gtctcttctg
    52441 gaagcatatg aaattctttc tcagccagct gaaaaatctc tttttctggt ttttggaaac
    52501 ctgttgcttg agaaatgagc atattgcggg aaggaatcca attgttcaat tgaagttgct
    52561 tcagtttttt tgtctgatgg tcagtcggcc catttgtgat aattcctaat gggacattct
    52621 tttccttcaa gaaatcgagg gtttttttga cctcctcatg caagcagata ttatcaagtt
    52681 cttcctcgta aattttttga aataagaggc catcttcttc cgtaatatga gggtagtcta
    52741 aatcttttag tgattggctg atccgatgtg ctcgcatata ttctagtgtc caatctcctg
    52801 ccattacttt tgggaagttt tcatcactat gatggcggaa ccggatatat agtggatgca
    52861 tatctgcatc tgaaacaagc ggaacgactc ttttgaccgc gtttcgaaat ggctgctgct
    52921 gatcatacat cgtatcatca acatcaaaca caactgattt aatcaaaatt ttacacgcct
    52981 ttcggttttc attgattttt tcacaaatcc ttgactattg taacagaaaa aattgtctaa
    53041 aaaagctgat aaataaaggt ttttcttact atatttctat ttccttgaca gaataaggtt
    53101 tttatatttc agtttttttc actaaaaatc gtgaaacacg tcaataagtt tgatgaacaa
    53161 attaatcatt atggtagaag tcgattgatt tattttaatg caatctctgt ggaattttcg
    53221 aaagatttat ttttttaata cagtctcaat ataactattc aaacataaaa taacgttatt
    53281 tgatttattt gtttatagta aattttattt agtaatcatt gctttaaaat actacgctta
    53341 tataacagag aaaataagcg ttataataaa gaacagtatt atttaaaatg acagaaaaca
    53401 ttactattaa agaaataaaa aacataaaaa atcttaagaa atcggtttca tttagctttt
    53461 gagataaaat aatagtacag ttttatacgc tgtttcatat gtttagaaaa tttgaattag
    53521 atttgaaaac tctaattttt aaatgataaa aaaagcgaga atgctggttt tactgtatat
    53581 taaccttaga taactttcat ttttttaaaa aaattatgaa aaaaaggtag ctatttaaaa
    53641 gttgattatg tataatagaa acagatggga gattattatg atcttctgtt acaaaaaagt
    53701 gattcttatc acgaaaagga gtggtgcacg ttgttaacat tatatacttc cccaagttgt
    53761 acttcctgca gaaaggctcg tgcgtggttg caagaacatc agattccatt tgttgaacgt
    53821 aatatttttt cagaaccatt gaacagttct gaattaaaag cgattttaca aatgacagaa
    53881 gacggaacag aagaaatcat ctcgacacgt tccaaagtat tccaaaaact gaacatggat
    53941 ttagatgatc tgccgttaca agaattattg gaacttgtgc aaaacaatcc tggattgctt
    54001 cgccgtccaa tcatgattga tgataaacgt ctgcaagttg gttttaatga agatgaaatt
    54061 cgtcggttcc taccgcgcga tgtacgacaa ttagaattac gtcaagcaca attgatggct
    54121 ggattataga aggataatac gaaacttgga aaaga
//
LOCUS       DACEEZ010000011        51744 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20313_2017
            SAMN12639966-rid9879223.denovo.011, whole genome shotgun sequence.
ACCESSION   DACEEZ010000011 DACEEZ010000000
VERSION     DACEEZ010000011.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639966
            Sequence Read Archive: SRR10023158
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 51744)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 51744)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741683.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741683.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:44:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,016
            CDSs (total)                      :: 2,962
            Genes (coding)                    :: 2,889
            CDSs (with protein)               :: 2,889
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 36 of 73
            Pseudo Genes (incomplete)         :: 29 of 73
            Pseudo Genes (internal stop)      :: 18 of 73
            Pseudo Genes (multiple problems)  :: 8 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..51744
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639966-rid9879223.denovo.011"
                     /isolate="E20313_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(115..918)
                     /locus_tag="IWO89_000710"
     CDS             complement(115..918)
                     /locus_tag="IWO89_000710"
                     /EC_number="1.1.1.69"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate 5-dehydrogenase"
                     /protein_id="HAQ0455484.1"
                     /translation="MEFSMDMFRLDGKVALVTGAVYGIGFEIARSLATAGAKIVFNNL
                     NQESVDEGIAHYKEAGIDAKGYVCDVTDEEAVQAMVQQIKEDVGSVDILVNNAGIIKR
                     TPMIEMDAADFRQVIDVDLNAPFIVSKAVIPDMIEKGGGKIINICSMMSELGRETVSA
                     YAAAKGGLKMLTKNIASEYGQYNIQCNGIGPGYIATPQTAPLRETQENGERHPFDQFI
                     VGRTPAARWGEPIDLAGPSVFLASSASDFVNGHVLYVDGGILAYIGKQP"
     gene            complement(938..1780)
                     /gene="kduI"
                     /locus_tag="IWO89_000711"
     CDS             complement(938..1780)
                     /gene="kduI"
                     /locus_tag="IWO89_000711"
                     /EC_number="5.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-dehydro-4-deoxy-D-glucuronate isomerase"
                     /protein_id="HAQ0455485.1"
                     /translation="MQEMETRYTHSPEDIRHYSTEQLRKEFLVEKVFEPGKISLTYTH
                     NDRMIFGGVTPTEKELEITLDKELGVDYFLERRELGVINIGGPGFIEIDGQKEEMKKQ
                     DGYYIGKETRHVVFSSQDAENPAKFYISSVPAHHKYPNVKISIDQVKPMETGEGLTLN
                     ERKIYQYIHPNVCESCQLQMGYTILEPGSAWNTMPCHTHERRMEAYVYFDYANEDTRV
                     FHMMGKPDETKHLVVDNEQAIISPSWSIHSGVGTSNYSFIWAMCGENITYTDMDMVQM
                     DQLK"
     gene            complement(1796..2803)
                     /locus_tag="IWO89_000712"
     CDS             complement(1796..2803)
                     /locus_tag="IWO89_000712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar kinase"
                     /protein_id="HAQ0455486.1"
                     /translation="MGKVVTLGEIMLRLSTEQGTRIDSSERFAAHYGGGEANVAISLA
                     NYGHEAIFATKVPDNALGKAVKKHLQCYGVDTKQVLSGGSRLGTYYMETGSGERAASV
                     IYDRAGSSFAEMDHLEWSLEELFQGIDIFHVSGITPALSKKWQKMTKQVIEAAKKAGC
                     LISFDINYRGKLWTQQEASEVIHQLLPLVDICSAGKMDALYLLGIEKAPEAEEQPLIY
                     YYQKMQEKFPNIQVFYSTKRTVHSASENELKGTLWMNHEYVESQVHRITPIVDRVGGG
                     DAFAGGVLHGILSNMSPQEIIDFATAASALKHTIHGDCNQFSQEEVAQFLACGSGKII
                     R"
     gene            complement(2810..3454)
                     /locus_tag="IWO89_000713"
     CDS             complement(2810..3454)
                     /locus_tag="IWO89_000713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 4-hydroxy-2-oxoglutarate
                     aldolase/2-dehydro-3-deoxy-phosphogluconate aldolase"
                     /protein_id="HAQ0455487.1"
                     /translation="MKKIDILSRLKNAGVIAVVRGKSKEEALNACHAIIKGGLTGIEL
                     TFTVPQADQVIKELLSFYKDQPEIVIGAGTVLDAVTARLAILAGAEYIVSPSFDQETA
                     EMCNLYQIPYLPGCMTITEIKTALKSGVDIVKLFPGSAYGPSIISAFKAPMPQVNIMP
                     TGGVSLDNMKEWFDAGVVTVGVGGNLLAPAATGDFDKVTEVAQQYAAKMKEIKR"
     gene            complement(3554..4312)
                     /locus_tag="IWO89_000714"
     CDS             complement(3554..4312)
                     /locus_tag="IWO89_000714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator"
                     /protein_id="HAQ0455488.1"
                     /translation="MENKKPYGTVLIKASKILDYLAENQDVSLQTIAKGVGMTSSTVL
                     KILDTLLMIGYVNKNSEKNYRLGAKLIRYANKNIEQIDLVELTLPFLEQLQQKIDETI
                     HLGVLDNNEILYVNKLDPKHQTIRMSSKIGITRPLYNSAMGKAVLAEFSEEQYTDYLE
                     KHPLIPYTEYTITNALRLKKEIKTVKETHIAFDDEEIERDIFCIGASIVKNEQIIGAF
                     SVSMPKYRLTDETKEQIISALKQTKSEIEQRIQK"
     gene            complement(4439..5590)
                     /gene="fucO"
                     /locus_tag="IWO89_000715"
     CDS             complement(4439..5590)
                     /gene="fucO"
                     /locus_tag="IWO89_000715"
                     /EC_number="1.1.1.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactaldehyde reductase"
                     /protein_id="HAQ0455489.1"
                     /translation="MSNRMILNETSYFGKGAIESIVPEFQQRGFGKAAVITDKGLIEH
                     GIATKVTELLDKAAIAYALYDGIVPNPTIQNVKDGVAFVKEAEADCLIAIGGGSPIDT
                     AKAIGIILTNPEFSDVISLEGVADTKNPCLPILAIPTTSGTAAEVTINYVITDEVNHR
                     KFVCVDPHDIPIVAFIDSDMMMGMPKKLAASTGMDAMTHAIEGFITKGAWEMTDMLHL
                     KAIEIIGHSLEASVDGDQNGREKMALGQYIAGMGFSNVGLGLVHGMAHPLSAWYNIPH
                     GVACAALLPTVMKYNKEYTGEKYREIALVLGIKGAAEMSLEDVREAACGEIDRLSKAV
                     GIPETISELGVKEADIPAIAEDALRDVCTPGNPRETTVEEIIALYQSLM"
     gene            complement(5672..5983)
                     /gene="rhaM"
                     /locus_tag="IWO89_000716"
     CDS             complement(5672..5983)
                     /gene="rhaM"
                     /locus_tag="IWO89_000716"
                     /EC_number="5.1.3.32"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose mutarotase"
                     /protein_id="HAQ0455490.1"
                     /translation="MVKKAFCMKVYPEKQEEYKKRHDELWPEMRTALREHGVIDYSIY
                     LDPSTSLLFGYLVIEDEKLWEKMANTSINQKWWKYMEDIMETNPDASPVSWELMPVFE
                     L"
     gene            complement(5985..6830)
                     /gene="rhaD"
                     /locus_tag="IWO89_000717"
     CDS             complement(5985..6830)
                     /gene="rhaD"
                     /locus_tag="IWO89_000717"
                     /EC_number="4.1.2.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnulose-1-phosphate aldolase"
                     /protein_id="HAQ0455491.1"
                     /translation="MKKIDILQAPYVKEMIETTSNLYRLGWDERNGGNISYLLKEEEI
                     TPFLDPEKVIRRISMIFDASRLAGCYFIVTGSGRYFKNVASEPAENLGIVRVAEDGKT
                     LELLWGLENECLPTSELPSHFMSHIARLEVDPDNRIVMHCHASHLLAMSFTHELDERS
                     FSRTLWQMCTECLVVFPEGISIIPWMVPGTNEIGEATAEKMKETRLVLWPQHGIYGSG
                     KDMDEVFGLIETAEKAAEVYTYVKAQGPILQTITDENLWHLADAFGVTPKAGYLEEVH
                     TKAGV"
     gene            complement(6844..8100)
                     /gene="rhaA"
                     /locus_tag="IWO89_000718"
     CDS             complement(6844..8100)
                     /gene="rhaA"
                     /locus_tag="IWO89_000718"
                     /EC_number="5.3.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose isomerase"
                     /protein_id="HAQ0455492.1"
                     /translation="MNTIERNYEQAKEKYATIGVDTDAVLEKIQDIKISMHCWQGDDV
                     KGFLTPDGELTGGIMATGNFPGAARTPEELRQDLEKAYSLIPGKHKLNLHAIYLDTEE
                     SVDLNEIEPKHFEKWVEWAKKEEIGLDFNPTFFSHPMMKDGFTLAHPDKEVREYWIEH
                     GKRSRKIAEYFGKETGQTSINNFWVPDGMKDNPIDRFTPRERLMESLDEIFTEELDER
                     YVQDAVESKLFGLGAEAYTVGSHEFYMGYGLTRDKLVCLDAGHFHPTEVISNKLSSLA
                     LFSKGIMLHVSRPVRWDSDHVVLMDDELQEIAKELVRNELLEKTNIGLDFFDATINRI
                     AAWVIGTRNTQKALLKAMLEPVERLKEMELAFDFTSRMAYTEELKDFPYADVWNYFCY
                     KNQVPVGLDWLEEVQEYQKDVLELRK"
     gene            complement(8097..9557)
                     /gene="rhaB"
                     /locus_tag="IWO89_000719"
     CDS             complement(8097..9557)
                     /gene="rhaB"
                     /locus_tag="IWO89_000719"
                     /EC_number="2.7.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnulokinase"
                     /protein_id="HAQ0455493.1"
                     /translation="MNPTFVAIDIGASSGRLMKSTRDAKGSLILEEVHRFKNGFRNKD
                     GHDRWKIDYLIKEILIGFEKLKQTGIDRLFAGIDTWAVDYCLIDEQGDRINDPIAYRD
                     KRTNGAVEQFSEIYSLDKLYEQTGIQIQPFNTLFQLFAEEKENLAKAHKLLLIPDYLG
                     YVFTGNMVTEKTNASTMQLLNAGTKEWEIELLEKIGVSKELFPPLTEPGTILGDLQKE
                     QFSKYDLPDVTFITVASHDTASAVLGTPGQGERWGYISSGTWSLLGIETKIANVSRNA
                     FSENYTNEWGAHNTVRFLKNIMGMWLIQEVARMQEYEYSYAELAELAAKEKPLQQVME
                     INDPRFLNPPNMIEEIQQYCFETEQPVPVSSGAIARCVYDNLALCYAQELIKVEHLTG
                     TKLEKLHIVGGGSNNRLLNQLTADYAGIIVEAGPSEATAIGNVAMQMLSTGQFSNIEQ
                     ARNVIKQSFPCETFYPDENKQNTMEWYVNKIKGVIV"
     gene            9710..10579
                     /locus_tag="IWO89_000720"
     CDS             9710..10579
                     /locus_tag="IWO89_000720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HAQ0455494.1"
                     /translation="MDYWEKANHSWSEDSKRVILTPTQRSRNLFFYIQEIGYFKAPIP
                     YYTERAHLPSYLIKFTLSGKGSLTYNDNQQQLKAGDLFFIDCQEYQRYQTISEEPWEM
                     DWIHLNGQNVQSFYQEFTKDGHNTFHSTGDPSENKIHHLITELIHMQQGTNARTDFQS
                     SVAIHELLNELILQKYQLDFEEEDIPAYVYKLKQYLDEHFKETITLDQLEQQFLLNKY
                     QLSKDFSKFIGSPPIDYLISKKLSYAKDLLRYTEFTVRMISEEIGIENFAYFSRLFKA
                     KTGLSPRDFRKFS"
     gene            complement(10705..11604)
                     /locus_tag="IWO89_000721"
     CDS             complement(10705..11604)
                     /locus_tag="IWO89_000721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HAQ0455495.1"
                     /translation="MLQKKISGEQVNTMVSDQYEIYHVKDVVSQNKTIYHHHDFYEIH
                     ATLKGEATFYLNGRQFTIKAGSVLLIHSNDLHRIVRQSSDVFERVYIFVTPSFLESRS
                     TPWSNLSACFQPMGEKRSRVLQIEQEVLKQKLSFIDHPPDKKIYGEDIKYEQALVEYL
                     IFLNQVVQKEESAQEQNAVVPNERVEQMLQYISKNLSHPLSLEEMEKQFFVSKYHITR
                     EFKKYTGFTFHQYVMKKKLIYAKHLLREYRSSSAVYSKSGFSSYPHFLKAFKREFGIT
                     PKEFLKRDTQKQLVHYEHFEDEQ"
     gene            complement(11769..12593)
                     /locus_tag="IWO89_000722"
     CDS             complement(11769..12593)
                     /locus_tag="IWO89_000722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HAQ0455496.1"
                     /translation="MLNITIRGHDLSEVQTIEDLAEKTKEQGIHTLQLALGLSFPEIP
                     SGANEINSGMGNYVKRVLEKQEVTVGILSCYINMIHPDLTIRQELLTKFEQYLRYASS
                     FGASMVASETGCVLPEIQYTEENFTDEAFAEAVSVIRRLVKAGEKYQMMVGIEPGLNH
                     PVYSLARVEQLIQAVDSDYLGIILDPTNLITSSDYQEQVQLVEEAFERFGEKICAVHL
                     KDFRVEQEKIVPVNLGDGVIEYTKIKEIIMKNRPYLYVVLEETKDDGIRYGRSLLE"
     gene            complement(12604..13899)
                     /locus_tag="IWO89_000723"
     CDS             complement(12604..13899)
                     /locus_tag="IWO89_000723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease"
                     /protein_id="HAQ0455497.1"
                     /translation="MAIEAGLVLFFVFVVLLVLGMPIAISIAFSSMMTLLLVIPFDVS
                     AFSSAQKMVGSINSFSLIAIPFFVLSGIIMNNGGIAKKLVDFAMLFVGRVPGALAHTN
                     VLGNALFGSISSSAIAASTAIGGVLIPQQVEEGYDRKFATAVNIASAPTGMVIPPSTA
                     FIMYSLVAGGASISALFMGGYLVGALWALGIMLVAFIVAKKNKYPVVEKGQMAHAGKI
                     IKEAIPSLLLIVIIIGGILTGIFTAVEASAIAVIYSLLIAMFYYKTVKLKDIPNMLFQ
                     SVAMSGTIMFLLATSSMMSFAMAFTGIPQAISSLIMGVTDNKFIILLLVNLVLLLVGM
                     FMDVAPAILIFTPIFLPIVTSVGVDPVHYGLFSIMNLCVGSITPPVGTGLYVGASVGG
                     VKAEAMLKPLLPFYGVILVVLFLITYFPQIVMWLPNMLS"
     gene            complement(13915..14394)
                     /locus_tag="IWO89_000724"
     CDS             complement(13915..14394)
                     /locus_tag="IWO89_000724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease"
                     /protein_id="HAQ0455498.1"
                     /translation="MKMILNKVLEILGAVLLVGMVAVVLWQVFSRTVLGNPNTVTEEL
                     VRFGLVWFAMLSSAYVVGQKAHLAVTVLSEKLTGKKSKILEVVVQSLFLVFAAMIMVY
                     GGWNAVTLTMGQISPSLSIPMGYVYLSVPVSGVLIIIYSLINLIDTLSNKPAIQEKI"
     gene            complement(14400..15427)
                     /locus_tag="IWO89_000725"
                     /pseudo
     CDS             complement(14400..15427)
                     /locus_tag="IWO89_000725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294179.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein"
     gene            complement(15444..16550)
                     /locus_tag="IWO89_000726"
     CDS             complement(15444..16550)
                     /locus_tag="IWO89_000726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase"
                     /protein_id="HAQ0455499.1"
                     /translation="MIIKEEIFVPKELVKEKIDLLVENLTTIKDDNGEFLLDFDGLKV
                     DDKSWTVWNWPQGVGLYGIYKNYQMTKNPRAYQVVNEWFEDRMEEGAPPKNVNTMAPL
                     LTMAYLYEDTKDSRYLPYLEQWAEWVMNDMPRTNEDGLQHATYGPENKNQLWDDTLMM
                     TVLPLAKIGKLLNRPEYLEEARYQFMIHIKYLMDKRTGLWYHGWTFEGNHNYAEAFWA
                     RGNCWLTIAIPEIIEILELSKEDALRKLLIETLEAQVRALKTYQSESGLWHTLLDDPS
                     SYVESSATAGFAYGILKAVHKRYLPQEYKEVAYRAIQGLLEQIDEKGEVQNVSIGTGM
                     GDSLDFYRNIGITAMPYGQSLTVLCLTELLVSYC"
     gene            complement(16779..19376)
                     /gene="adhE"
                     /locus_tag="IWO89_000727"
                     /gene_synonym="adhC"
     CDS             complement(16779..19376)
                     /gene="adhE"
                     /locus_tag="IWO89_000727"
                     /gene_synonym="adhC"
                     /EC_number="1.1.1.1"
                     /EC_number="1.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acetaldehyde-CoA/alcohol
                     dehydrogenase"
                     /protein_id="HAQ0455500.1"
                     /translation="MAKVIEKEKTKTIDVQAMIDDLAEKANAALKEMESFDQEKVDHI
                     VHEMAMAALDQHMPLAKMAVEETGRGVYEDKAIKNMYASEYIWHNIKHDKTVGVINED
                     VQKGLIEIAEPVGVVCGVTPTTNPTSTTIFKSMIALKTRNPIVFAFHPSAQKSSAEAA
                     RVVRDAAIAAGAPENCIQWIEHPSIEATSMLMNHPGIAIVLATGGAGMVKSAYSTGKP
                     ALGVGPGNVPAYIEKTAKIKRAVNDLIVSKTFDNGMICASEQAVIVDKEIYAAVKAEF
                     QAHQVYIVKPDELQKLEDAVMNEGKYAVNPSIVGHSAMEIAKLAGISVPKGTKMLIAE
                     LEGVGPDYPLSREKLSPVLAMIKANNTDHAFDLCEGMLNLGGLGHTAVIHSENEELHV
                     KFGLRMKACRILVNTPSAEGGIGDIYNEMIPSLTLGCGSYGKNSVSRNVSAVNLINVK
                     TVAKRRNNMQWFKLPPKIFFEKNSLLYLEKMENVERVMIVCDPGMVQFGYCDTVREVL
                     ARRKNDVKIEVFSDVEPNPSTNTVYAGTKLMADFKPDTVIALGGGSAMDAAKGMWMFY
                     EHPDTSFFGAKQKFLDIRKRTYKIDKPEKTQFVCIPTTSGTGSEVTPFAVITDSETHV
                     KYPLADYALTPDVAIVDPQFVMSVPASVTADTGMDVLTHAIESYVSVMASDYTRGLSL
                     QAIKLVFDYLEKSVKTPDMESREKMHNASTMAGMAFANAFLGICHSVAHKIGGEYGIP
                     HGRTNAILLPHIIRYNAKDPQKHAMFPKYDYFRADTDYADIAKFLGLKGETTEELVEA
                     LATAVYELGKSVGINMSLKAQGVTQETLDTTVDRMAELAYEDQCTTANPKEPLISELK
                     QIIIDAYNG"
     gene            complement(19681..20061)
                     /gene="yajC"
                     /locus_tag="IWO89_000728"
     CDS             complement(19681..20061)
                     /gene="yajC"
                     /locus_tag="IWO89_000728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HAQ0455501.1"
                     /translation="MGTLPMLVMFIALLAMWFFMSRSQKKQQQERQNLLDSMKPGDSV
                     VTIGGLHGVVSEIDNDKRTVVLDCEGIFLEYDRAAIKTVKPGTVVTNDATTTVETTEE
                     PAETVEVKTETTETLEETEEKEEK"
     gene            complement(20158..21303)
                     /gene="tgt"
                     /locus_tag="IWO89_000729"
     CDS             complement(20158..21303)
                     /gene="tgt"
                     /locus_tag="IWO89_000729"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010769586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HAQ0455502.1"
                     /translation="MTQPAIRYRLIKKEKHTGARLGELITPHGTFPTPMFMPVGTLAT
                     VKTMSPEELKEMGAGVILSNTYHLWLRPGEDLVEEAGGLHKFMNWDQPILTDSGGFQV
                     FSLSDMRNIEEEGVHFKNHLNGSRMFLSPEKAINIQNKLGSDIMMSFDECPPFDESYD
                     YVKKSVERTSRWAERGLKAHANPDRQGLFGIIQGAGFEDLRRQSAKDLVGMDFPGYSI
                     GGLSVGEPKSEMNRVLEFTTPLIPENKPRYLMGVGAADSLIDGVIRGVDMFDCVLPTR
                     IARNGTCMTSQGRLVVKNAKYAHDFRPIDEKCDCYTCKNYTRAYIRHLIKCDETFGIR
                     LTSYHNLYFLLNLMKQVRQAIMDDNLLEFREAFFEEYGFNKENAKSF"
     gene            21521..22219
                     /locus_tag="IWO89_000730"
     CDS             21521..22219
                     /locus_tag="IWO89_000730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF975 family protein"
                     /protein_id="HAQ0455503.1"
                     /translation="MQSSAELKRQAKESLKGRWGQAVLLNLIPTLIGIATILLIMIPI
                     ILFATTIYDGSASVSEATTSGGGSSGGGGGIISSIISALFMSGISWTYLDILRGQKDQ
                     IEPFKDAFRGFKGLFIGGVISLAVLISIFTTLWGLLLVIPGIIKSYSYSQSYFIYYDI
                     VQETGQTPKVLDTITASRKLMDGYKGQLFWLDLSFIGWHILALATAGIGYLWLSPYIS
                     ATKAAFYEQLPKDI"
     gene            complement(22387..25026)
                     /locus_tag="IWO89_000731"
     CDS             complement(22387..25026)
                     /locus_tag="IWO89_000731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HAQ0455504.1"
                     /translation="MEEYKQTIEEVKKVTEADPDTGLSSQEVDERRKKQGLNKFDEAP
                     KESMIKKFFRSLSDFTTIILLVAAVISFYTAFATEHGDLFEGLLIIAIVVINSVLAIV
                     QEGNAEKALESLQDMNKQTATVIRDGKVTTVESEQLVVGDILVLESGDAISADARLIE
                     ASQLRVEESALTGESEAVEKDAAFIAEEDESLGDQLNMVFKGCTVAAGRGKAIVTAIG
                     MATEMGKIADLLNENTMQKTPLQVRLNQLGKRISMIALAAAALVFVIGELQGEPLLEM
                     FMTSISLAVAAVPETLTVIVTLTLAYGVQKMARKHAIIRQLPAVETLGTANVICSDKT
                     GTLTQNEMRVRRVWSKEDEVTNIEDSMTNSAMEILKMAALCTDVTVEQEDDDLVIKGN
                     PTEVAIVRAVEENYHTKAELEEKYPRVNELPFDSERKMMTTVHQMGEKYISITKGAFD
                     VLAPRFSSGDVEQAAIVNDRFGKRALRVIAVGYATYDEEPQDISSEALEKDLRLIGLI
                     GMIDPPRPESKGAIKRAKKAGIKTVMITGDHVVTASAIAKELGILNDPSEALSGSELH
                     QLSDEELDARVKALSVYARVTPEDKIRIVKSWQRTGAVVAMTGDGVNDAPALKASNVG
                     CAMGITGTDVAQSAADMILTDDNFATIVDAVSQGRSVYQNIRKAINFLLSCNISEIFI
                     VLIAMLLGWGAPFTAVQLLFVNVVADGLPGFALGREPAEHGIMDQPPIPKNEGIFARG
                     LLQKIAINAGVFTIVTLFGYYLGSYVDTISPWVDASQHVGQTVAFLILAYSSILHVFN
                     VRSSQSIFKVNLATNKALVEMALLALAITTAVALLPFTQELFGLVHISLNHWFLVGIL
                     SIVPIAVNELIKFHRLPEAEEEE"
     gene            complement(25645..26676)
                     /gene="queA"
                     /locus_tag="IWO89_000732"
     CDS             complement(25645..26676)
                     /gene="queA"
                     /locus_tag="IWO89_000732"
                     /EC_number="2.4.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA preQ1(34) S-adenosylmethionine
                     ribosyltransferase-isomerase QueA"
                     /protein_id="HAQ0455505.1"
                     /translation="MLTTEDFDFDLPEELIAQTPLKDRDHSRLLVLDRKTGEMEDKHF
                     HEIIEELQPGDALVMNNTRVLPARLYGEKPDTGAHLEVLLLTNTQGDTWETLIKPAKR
                     AKVGTRISFGDGRLQAVVKEELEHGGRIIDFEYEGIFLEILESLGEMPLPPYIKERLE
                     DPDRYQTVYAKENGSAAAPTAGLHFTKELLDEIEAKGVELVYLTLHVGLGTFRPVSVD
                     KIEEHHMHSEFYRLTEEAAERLNQVRKNGGKIVAVGTTSIRTLETIGTKFNGEIKADS
                     GWTDIFITPGYEFKVVEAFSTNFHLPKSTLVMLVSAFAGRELTLSAYQHAIEERYRFF
                     SFGDAMFVK"
     gene            26869..28044
                     /locus_tag="IWO89_000733"
     CDS             26869..28044
                     /locus_tag="IWO89_000733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628506.1"
                     /note="Members of the family are the ATP-binding subunit
                     of ABC transporters for substrates such as betaine,
                     L-proline or other amino acids, choline, carnitine, etc.
                     The substrate specificity is best determined from the
                     substrate-binding subunit, rather than this subunit, as it
                     interacts with the permease subunit and not with substrate
                     directly; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="betaine/proline/choline family ABC transporter
                     ATP-binding protein"
                     /protein_id="HAQ0455506.1"
                     /translation="MIEFQNVSKIYKGGKVAVEDVNLSFEKGEFICFIGTSGSGKTTT
                     MRMINRMTDPTQGKILIDGKSIQEIDPVELRRQIGYVIQSIGLMPHMTIRENITLVQK
                     LLKVSQEERNKTAEKMIDLVELPREMLDRYPHELSGGQQQRIGVVRALAANQDIILMD
                     EPFGALDPITRDSLQDLVKDLQERLGKTIVFVTHDMDEAIKLASRIAIMSEGRVIQFD
                     TPENILRHPANDFVEELIGEDRLLQAKPNATRVGEVMLNTAITITPEKSLQEAIKLMR
                     EKRVDTLLVTDNSNVLKGFIDVETLNKKRGKVSSVGDILNKHVFYVKESAYLRDTLQR
                     ILKRGLKYVPVVDEQNKVVGILTRASLVDIVYDVIWGEDPATTDVAESATPEESKEV"
     gene            28048..28689
                     /locus_tag="IWO89_000734"
     CDS             28048..28689
                     /locus_tag="IWO89_000734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAQ0455507.1"
                     /translation="MQQFFHQHGSEILSKGFEHLYISGLALIIGIIIAVPLGVLLTRF
                     PKAATIVIGLTSALQTIPSLALLALMIPLFGVGKLPAIIALFIYSLLPILRNTYIGMK
                     NVDWNYRDVAKGMGMTEFQSIFSVELPIAMPTIMAGIRLAAVYVIAWATLASYIGAGG
                     LGDLIFSGLNNYQPDLILAGTIPVTILALLADWLLGLLEHRLTPIALREENEE"
     gene            28686..29603
                     /locus_tag="IWO89_000735"
     CDS             28686..29603
                     /locus_tag="IWO89_000735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="osmoprotectant ABC transporter substrate-binding
                     protein"
                     /protein_id="HAQ0455508.1"
                     /translation="MKRILKMTMVLFSLLILASCSFPGLASNTDEDTISITGGITSEA
                     QILASLVAGMVEHYTDKNTAIINNLATTTINHQAMMNGDASISAARYTGTDLTTTLNL
                     PPEKDPKKAFATVKDEFEKRYGQTWFPSYSFENTYVFLVRKDTAQKYHLSKVSDLKNV
                     ADELVAGVDTSWINRKGDGYDGFQETYGFSFNSILPMQIGLVYDAVEAGKMDIVLGYS
                     TDGRIASYDLVMLEDDRQFFPPYDAAPVVDNKILADTPHLKEALNKLGHTISTEKMQE
                     LNYEADNNLVEPSVVAQRFLQENHYFEGK"
     gene            29600..30271
                     /locus_tag="IWO89_000736"
     CDS             29600..30271
                     /locus_tag="IWO89_000736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAQ0455509.1"
                     /translation="MKQLDKMNVFQQFFYYFQENGSYIFAQFIRHFLISIYGVLFAAV
                     VGIPVGIMISRRRKLANWVIRLANIIQTIPSLAMISILIIGLGLGVNVVIVTVFLYSL
                     LPIIKNTYTGMIQVDKNILDVGKGMGMTARQRLFMVELPLSVSVIMAGIRNALVVAIG
                     ITAIGAFVGAGGLGDIIIRGTNATEGTSIILAGALPTALMAIITDWLLGILERRLDPA
                     SRTSR"
     gene            complement(30812..31300)
                     /locus_tag="IWO89_000737"
     CDS             complement(30812..31300)
                     /locus_tag="IWO89_000737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannosyl-glycoprotein
                     endo-beta-N-acetylglucosamidase"
                     /protein_id="HAQ0455510.1"
                     /translation="MTRKISKIGASLLVLAVATTIDYRQSEAKTIGNVVYRETAKSML
                     KKAMDNQPESSYWFPEDSLAWSFGKDTDTKYNTSVVPLAERVSKATLPNMNVTQTEEV
                     KVVALSAMNSSINGNASRGIDIFYANVFSYWQYIDQLVYGGALLVKDHRIAKSSCDGC
                     SP"
     gene            complement(31501..31767)
                     /locus_tag="IWO89_000738"
     CDS             complement(31501..31767)
                     /locus_tag="IWO89_000738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Txe/YoeB family addiction module toxin"
                     /protein_id="HAQ0455511.1"
                     /translation="MSNYTVAIKNSAKVDLRKIKQTNLKKQFEEVIQTLKEDPYMPTQ
                     SFEKLRPTHEGRYSRRLNRQHRVVYKVDEENKVVEIYSAWTHYE"
     gene            complement(31767..32030)
                     /locus_tag="IWO89_000739"
     CDS             complement(31767..32030)
                     /locus_tag="IWO89_000739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HAQ0455512.1"
                     /translation="MANTTMNPSTARKNFYQLLKEVNENHTEIEIISDRSGNNAVLIG
                     LEDWRAIQETLFLEQTGTLDKVRDREKDDSSFTNIDDIDWEAL"
     gene            complement(32023..32295)
                     /locus_tag="IWO89_000740"
     CDS             complement(32023..32295)
                     /locus_tag="IWO89_000740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0455513.1"
                     /translation="MYWCCCIDSQALDSTFAKDDTLLPITLRLATASRKGVRSERGQR
                     SSLKRGLFLYVKKRKSLLNLSALLYFFDVLNQVRYNYIRKRRRDNG"
     gene            32506..32691
                     /locus_tag="IWO89_000741"
     CDS             32506..32691
                     /locus_tag="IWO89_000741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HAQ0455514.1"
                     /translation="MALSYGNKKWSLDEAEIPTYEDTWHLRVEVKEEAENIGGCTLFG
                     KGFGNYDQDIKIKMFYS"
     gene            complement(32833..34656)
                     /locus_tag="IWO89_000742"
     CDS             complement(32833..34656)
                     /locus_tag="IWO89_000742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HAQ0455515.1"
                     /translation="MDYLKRLLVGKPLKSAENDEHKLTRFAALALLSSDALSSIAYGT
                     EQIVVVLVALSAAAIWYSLPIAAFVIILLISLTLSYRQIIHAYPHGGGAYVVSSENLG
                     KNAGLISGGSLLIDYMLTVAVSVSAGAEAITSAVPALYGHQVAISVTIVLLLMMLNLR
                     GLRESASFLLFPVYTFILVISLLIVVGLYNIVTGAVPLQATALPGAAVPGVSIALILR
                     AFSSGSSSLTGVEAISNAVPFFKKPRAKNAAATLTMMALILGFFFVGITFINYWYGIV
                     PEKEVTVLSQIGKAVFGHGILYYVLQFATALILAVAANTGFSAFPVLAYNLAKDKFMP
                     HMYQDRGDRLGYSNGIITLALGSIVLLFIFHGSTERLIPLYSIGVFIPFALSQTGMVV
                     KWKKEGKRWLSKSIANITGAFISYAIIAILFVYRLGDIWPFFIIMPIVMFIFYKIHDH
                     YKKVAEQLRLENDAKLHDYDGNTVLVLVGNVTRVNIGALNYARSIGDYVVAMHVSLDE
                     DVEKEKEIEAEFKKHFPDVRFSIVHSSYRSIENPIIRYVDIVSKNAAKQNYTTTVLIP
                     QFVPNRRWQNILHNQTSLRLRLRLSWRENIVVSTYSYHLKK"
     gene            complement(35254..37959)
                     /locus_tag="IWO89_000743"
     CDS             complement(35254..37959)
                     /locus_tag="IWO89_000743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhgE/Pip domain-containing protein"
                     /protein_id="HAQ0455516.1"
                     /translation="MQSIKNTLRLYLLDWKRIFKNPIATLLIIAIMIIPSLYAWFNIK
                     ALWDPYGNTGELPIAVYSADKPAEFQGKEVAIGEQVIESLHKNKQLGWQFVDSKEQLE
                     DGVRSGKYYAGIYLPKDFSEDLLSFTSGDIKKPKIEYTVNEKINAIAPKITDKGASSI
                     QSQITNEFIKTASSTLLKVFNEIGYDIDTNLVSINKVKDMILSTDENLDTIDGYTKQV
                     LELQSQLPEIKEKLNKANEFVDYIPKVDEMGEKVVALNDKMPELKDQAKIILDLQEKI
                     PEIQNAGKQLAEIDGDFASIQETMNQGIDEAKQGLTIIQQVQDILPDVKNLENQASDL
                     ANQTKSGASQLKDAIPSITNSVDVTLKSIGQVATTTGSLIDTVRTAIEDGKLTDDEKK
                     NISNVLNGFIDGISQQQNAIDKLVEFLTKLQTSAGNTKLQPIIDQLNQLKPLLSNLQN
                     RLSALNTAVQNEDTKAIDELLDQIDAVVNNINGIIAGINTGQISSTVTSLLDQVIDTI
                     GTAQGALSKADQIDFDSLLSSTKATVTNAVAILEKYQKEMPAIGQEIHDANTMLNGNM
                     DTIVNGINEGADLYNNELPIIQQKLGLAADFIQNDWPALKNEITGTLTTVNEKMPEVE
                     TAVNAAADLINNDWPNIKAGIQKAADAIRKGEQEVDLGEVIKLLKLDATKESDFFTQP
                     VELQTNALYPIANNGSASTPFYTALCLWVGAVLLSSVATTEYYLGKKDRNNFTKREQF
                     VARMLTFLTMAIAQSLIVTLGNIFLLGVDVQNPVYSVSFAVLIALAFMMIVYVLAALF
                     DNVGKGIAIIILVLSISGGGGNYPIQVSGKFFQMINPFLPFTHAVNLLRESAGGIYWP
                     NATKAIWIMIGLFVVFGIVGTAIYPFIEEKMTKFKEAAHESHIFH"
     gene            complement(38265..39212)
                     /locus_tag="IWO89_000744"
     CDS             complement(38265..39212)
                     /locus_tag="IWO89_000744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent oxidoreductase"
                     /protein_id="HAQ0455517.1"
                     /translation="MRRFAINGYGEAQDVFEEIDAHPREVGSGHVRVEIKAFSVNPYD
                     VALRLGEMKEIRTLKFPYVPGNDGAGIVTEIGSDVTTVHVGDRVAVHAVGGTYGEEVV
                     LPSAKVAKIPDKMSWEEAAGMVTPGITAYNLINHLTEIQPTDTVMILGASGAVGSSLI
                     QLLHEKGIRILTSASSKNEEKVKKLGASAFAAYDKTNPGLQFADQADLVIDATKGSIK
                     GETGIQIMKPGGRYVALNDLPDLDLRQKKEGFYESFVPRKEYLDAEAFAGIIKAYQKG
                     AFHVFISMNLSASLKHVIQAHQLVEGHPPAGKIILSFEK"
     gene            39402..39671
                     /gene="rpsN"
                     /locus_tag="IWO89_000745"
     CDS             39402..39671
                     /gene="rpsN"
                     /locus_tag="IWO89_000745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S14"
                     /protein_id="HAQ0455518.1"
                     /translation="MAKKSKIAKAKKQQETIERYAALRQELKAQKDYEGLRKLPLDAR
                     PERLKNRDLIDGRPRGYMRKFGMSRIKFRELAHKGLIPGVKKASW"
     gene            complement(39847..40209)
                     /locus_tag="IWO89_000746"
     CDS             complement(39847..40209)
                     /locus_tag="IWO89_000746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HAQ0455519.1"
                     /translation="MVKRGDIYFADLSPVVGSEQGGTRPVLVIQNNLGNHFSPTIIVA
                     AITAKMAKPKLPTHIGIKATGTGIERDSVILLEQIRTIDKSRLKEKVCHLDRPIMAEV
                     DRALKISVGIEALTPVTK"
     gene            complement(40220..41341)
                     /locus_tag="IWO89_000747"
     CDS             complement(40220..41341)
                     /locus_tag="IWO89_000747"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HAQ0455520.1"
                     /translation="MVVAWHRPTKAVIHKKAITENVANEVARLPQGKELFAVVKANGY
                     GHGAIETAEAAVAGGASGFCVSNLDEGVELREAGFTQPILILNMVPYDALTVAVAHDL
                     SVTAGTREWLQAAAAVLEKSKLETPLSIHLKADTGMGRIGFCTPEEVKEAAAFIKESR
                     VLEWEGLFTHFSTADQADDTYWNLQKERFIEVLKKLPELPRYVHVSNSATALWHDETI
                     GNMIRYGVAMYGLNPSGHALPEVYPLQPALELVSELIQVKKLPAGEGIGYGETYITPE
                     AEWIGTIPIGYADGWPRKMQGFSLLVEGNYCETIGRVCMDQLMIRLPQEFPVGTKVTL
                     IGKNADKEITMQDIADQLGTIHYEVACGLGQRIPREYQE"
     gene            complement(41357..41707)
                     /locus_tag="IWO89_000748"
     CDS             complement(41357..41707)
                     /locus_tag="IWO89_000748"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="holo-ACP synthase"
                     /protein_id="HAQ0455521.1"
                     /translation="MIKGIGIDAVELPRITRLIEEKPKFIARILTSDEMKLFQSLPFH
                     RQVEFLGGRYACKEAFSKAWGTGIGKVTFQDVEILKNENGQPVVTRSPHEGNVWVSIT
                     HTNETAFAQIILED"
     gene            complement(41837..43348)
                     /locus_tag="IWO89_000749"
     CDS             complement(41837..43348)
                     /locus_tag="IWO89_000749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HAQ0455522.1"
                     /translation="MKFKELELSPELLKSVERAGFEEATPIQAETIPLALAGKDVIGQ
                     AQTGTGKTAAFGLPMLEKIDPDRHELQGLVIAPTRELAIQTQEELYRLGRDKKVRVQA
                     VYGGADIGRQIRGLKDRPHIVVGTPGRMLDHINRHTLKLGTVQTLVLDEADEMLNMGF
                     LEDIEKIISQVPDQRQTLLFSATMPPAIKNIGVKFMKNPHHVKIKAKEMTADLIDQYY
                     VRAKEYEKFDIMTRLFDVQTPELTIVFGRTKRRVDELARGLEARGYRAEGIHGDLSQQ
                     KRMSVLRSFKSGHLDILVATDVAARGLDISGVTHVYNYDIPQDPESYVHRIGRTGRAG
                     KGGMSVTFVTPNEMDYLHVIENLTKKRMTTLRPPTEKEAFKGQLGAAVEQIETKLAEN
                     GLDKYLQTADKLLEEYSAQDLVALLLKTTAKDPADAVPVKITPERPLPMQKKGYNKNG
                     KRGGGNNRNRRDGGNYRGNKSKGGYSKNNNSQKDGGKRHNDKKRGFVIRSNND"
     gene            complement(43670..45022)
                     /locus_tag="IWO89_000750"
     CDS             complement(43670..45022)
                     /locus_tag="IWO89_000750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HAQ0455523.1"
                     /translation="MNLTIQEIAQAVGSECSALTKITNVEFDSRKITEGSLFVPLAGT
                     RDGHEFIQQAITNGASASFWSLPVEQAPKNFPVIPVADPLKAMQQLATYYLEKVGAEV
                     VAITGSNGKTTTKDLTASVLSEKYKTYKTQGNYNNNIGLPYTILHMPQDTEKIVLEMG
                     MDHANEITELSLMAQPKAAAITMIGEAHIENLGSREGIAKAKMEITDGLVSDGLLVVP
                     ADEPLLTPLTATLVQTVTTFGIGNGDVHAEVTAKGKAQTDFVVEGETFTIPLPGSYNV
                     TNALIAYTIGRFFGLSIPEIRQGLAHVSITQNRTEWLTAGNGAAILSDVYNANPTAMG
                     LVLDTFKNLPTKGRRIAVLADMLELGKESLNMHAQMSEHIDPEKFAMIFLYGEQMHAL
                     KEELNQKYPQLPVFYFEKEKNKLIDAIKQNLEATDSIMLKGSNSMGLFEVVEQLQQMK
                     "
     gene            complement(45175..46251)
                     /locus_tag="IWO89_000751"
     CDS             complement(45175..46251)
                     /locus_tag="IWO89_000751"
                     /EC_number="6.3.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HAQ0455524.1"
                     /translation="MKITLLYGGRSAEHEVSILSAFSVLNAIYYNYYQVQLVFITKEG
                     QWVKGPLLTEKPASKDVLHLSWDPSGQTEEGFTGKVINPGEIKEEGAIVFPVLHGPNG
                     EDGTIQGFLETLNMPYVGAGVLTSACAMDKIMTKYILQAAGVPQVPYVPVLKNQWKEN
                     PKKVFDQCEGSLLYPMFVKPANMGSSVGITKAENREELQNALATAYQYDSRAIVEQGI
                     EAREIEVAVLGNEDVRTTLPGEVVKDVAFYDYEAKYINNKIEMQIPAEVPEEVYQKAQ
                     EYAKLAYTMLGGSGLSRCDFFLTNKNELFLNELNSMPGFTEFSMYPLLWENMGLKYGD
                     LIEELIQLGMNRYHQRQSFFEKNE"
     gene            46447..48192
                     /locus_tag="IWO89_000752"
     CDS             46447..48192
                     /locus_tag="IWO89_000752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AarF/ABC1/UbiB kinase family protein"
                     /protein_id="HAQ0455525.1"
                     /translation="MAQTNPRSRLAEIIRVFINYNVVPNFVQQKNPEQVKKAFEELGP
                     TFIKIGQMLSVREDLLSSAFTQTFKTLQDSVPSDTFSTVKKTIETELSLSLSDIFDDF
                     SKSPFASASMGQAHRAKLKNGDAVVVKIQHPNIAEEIRLDLQLFERAIPLIKYIPETS
                     VVDLKGVLQEVKRSLINEMDFLKESQNGEQFYQKNNGWKEIRSPKIYDAFCSKKVIVM
                     EEMSGKNLNHLMNAENKTETFITGIQNKQLKQEVAKLLVENFMKQVFDDGFFHADPHP
                     GNLLFHVLTKEEQTQASRKTETVHEKEFGSFAFRASTSAEDPVAPYTINYIDFGMMGH
                     LSAGLRQKLTQAVLALYTKDAYRIEKAVLRLCQQEGSFDESRFHQELTSFLEQYYDSP
                     IDEINLQEVFTHVVTICHQNNLQFDRDITLLLKAFGTLEGVIRVLDPEVSLMEVASPF
                     AQHYFLTHLDVEDTLKQSGLDLLEGMKAAPKIPQQLHHLLEMWTSGQGKVNLELKKQD
                     KLLSRIESMINRLVFGMILAALIVGSSLLVQAAPVENAEVVSLLGIFTYAIAAFVIIF
                     LAIDALIQMYKKRKK"
     gene            complement(48472..48909)
                     /locus_tag="IWO89_000753"
     CDS             complement(48472..48909)
                     /locus_tag="IWO89_000753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaiI/YqxD family protein"
                     /protein_id="HAQ0455526.1"
                     /translation="MRLFIDGDGSPVKEDVIKLGESYGLDVVIVTSVDHYTTKEYANH
                     VQIVYVDRGADSADYKIVGMVKKNDFLITQDYGLASLVLPKGVRVFHHSGKEFDEANI
                     GTLLEQRYHSGKLRKAGIRTKGPKPFTEKDHMSFRQALKKAID"
     gene            complement(48893..50266)
                     /locus_tag="IWO89_000754"
     CDS             complement(48893..50266)
                     /locus_tag="IWO89_000754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0455527.1"
                     /translation="MFEKLKRFGRNFTQTTPFILSNTVIFIPYLLFLGLTDSHNWLTI
                     LPFTLFYAFRMTGLFLVNSLRFGLDSYTLLMIALLIGGAGSLLGILGVFYFPLFYFSS
                     VLLGLSAAWLVPANTTVNFHEKTQGFINMDGKKYLFAAIWLFLLYQAIELPAPTQIAA
                     SFTIYTLFYVMAYHTVSHYPRYELDFKDIKRNLVATRELVLFLIFFGLLFLLRNARLL
                     FDERLFDLAIYGFLLLFILFVFVLGHQKKEWKLPSWLNLFTFLNGMLGNFLFLFSSFY
                     VGILYGLEKLSIFMYLPYAAGLIAAKIVGPWILKRLTWSPLAMQLLGLSSSLLVLLIP
                     NGFAIGVFLLSFWHVVTGSWLNKEYYQTDTAIPMDRRIVVKYTTQNKGSVIHQFLLMT
                     LLFVLAKEMGEPIHLLLITLNHESVPIESVQLLILAKWINVGLLLSGLVTVYHLWKKA
                     KKNEAIH"
     gene            complement(50588..51076)
                     /locus_tag="IWO89_000755"
     CDS             complement(50588..51076)
                     /locus_tag="IWO89_000755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAQ0455528.1"
                     /translation="MEKIKVTENELDELMAVIQEVWPEVFVPIIGQKQVDYMLKTYQS
                     KKQIQKELTEGVSYFLLKSEEKTVGYTAYEEINGKIYLSKLYLLNKVRGQGLTSSVFR
                     WYEELAAQTKEREIFLRVNQGNHQAIEVYKHEGFEITEELISDIGQGFQMVDYKMKKA
                     LA"
     gene            complement(51098..51700)
                     /locus_tag="IWO89_000756"
     CDS             complement(51098..51700)
                     /locus_tag="IWO89_000756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HAQ0455529.1"
                     /translation="MSKKGSVLQKCLQIMPIIGLFFFFVLILYAYHHGIFRSSTSLQA
                     FIQQFGKYAVMIFVLLQVIQVIIPILPGGISSVAGMLMFGNGFGLLYSCIGLVIGEAI
                     GFLLVRYYGVAFVQLILSPKKYQKFDELLTRKTKDIKKVLVVTLLLPFAPDDLICLVA
                     GLTKLSFREFMQIVIFLKPWSVGVYSMIMLFLFHQAQGHL"
BASE COUNT        14583 a        11282 c         9329 g        16550 t
ORIGIN      
        1 gcttctgtcc cagcctcttt tttcgaataa atgatgttta atcagctgtc cttcggtatt
       61 cgttcaatcc gctgatttca caacctctcc actaaacagt gtactaaagc tcttttaagg
      121 ttgttttccg atataggcaa gtatgcctcc gtccacatac aagacatggc cgttaacgaa
      181 atcagatgcg ctagaagcca agaagacaga tggtccagcc aaatcgattg gctcgcccca
      241 gcgtgctgct ggcgtacgtc caacgataaa ttgatcgaat ggatgacgtt caccgttttc
      301 ttgtgtttcg cgtaatggag cagtttgcgg tgtagcaata taacctggtc caattccatt
      361 acattggata ttgtattgac cgtattcaga agcgatgttt ttcgtcagca tttttaatcc
      421 gccttttgcc gcagcataag cactgaccgt ttcgcgtcct aattcactca tcatggagca
      481 gatattgatg attttaccgc cgcctttttc gatcatatcc ggaataactg cttttgaaac
      541 gatgaatggg gcatttaaat ccacgtcgat tacttggcgg aagtctgccg catccatttc
      601 gatcatcggt gtccgtttga tgatccctgc attgttgact agaatatcaa ctgaacctac
      661 atcttctttg atctgttgaa ccatcgcttg aaccgcttct tcatcggtca catcgcagac
      721 ataacctttt gcatcgatgc ctgcttcttt ataatgcgct ataccttcat caactgattc
      781 ttgattcaag ttattaaata cgatttttgc tcctgctgta gctaaagagc gtgcaatctc
      841 aaatccaata ccatagacag caccggtgac taatgctact tttccatcta aacgaaacat
      901 atccatacta aattccatca ttttctctcc cttatgctta ttttaattga tccatttgca
      961 ccatatccat gtctgtatag gtgatatttt ctccgcacat tgcccagatg aatgagtaat
     1021 tgcttgtacc tacacctgaa tggatcgacc agcttggcga aataatggct tgttcattgt
     1081 ctacaactaa atgttttgtt tcatctggtt tgcccatcat gtggaacaca cgtgtatctt
     1141 cattggcata atcaaagtag acataagctt ccatacgacg ttcatgtgta tgacaaggca
     1201 tcgtattcca agcgcttcct ggttctaaaa tcgtataacc catttgtaat tgacagcttt
     1261 cgcatacatt tggatggatg tattggtaga ttttacgttc attcagtgtc aggccttcac
     1321 cagtctccat tggttttact tggtcgatac tgatttttac atttggatat ttatgatgag
     1381 ctggaacaga actaatatag aatttagctg ggttttcagc atcttgagaa gaaaatacaa
     1441 catgtcgtgt ttcttttccg atatagtatc cgtcttgttt tttcatttct tctttttgcc
     1501 catcgatctc gatgaatcct ggtccaccga tattgatcac acctagttca cgacgctcta
     1561 agaagtaatc aacacctagt tctttgtcta atgtgatttc taattctttt tcagtaggtg
     1621 tcactccacc aaagatcatt cgatcgttgt gcgtataagt caaactgatt ttccctggtt
     1681 caaatacctt ttctactaaa aattcttttc tcaattgttc cgttgaataa tgacggatat
     1741 cttctggact gtgtgtataa cgtgtttcca tttcttgcat tttcttctac tccttctatc
     1801 gaatgatttt tccagagccg catgctaaaa attgtgcgac ttcttcttga ctaaattggt
     1861 tgcagtcccc atggatcgta tgcttcaagg cagaagctgc cgtagcaaaa tcaatgattt
     1921 cttgcggaga catattggat aaaatcccat ggagcacgcc acctgcaaaa gcatctccgc
     1981 cacctactcg atccacgatt ggggtgattc tatggacttg ggattccaca tattcatggt
     2041 tcatccacaa tgtccccttc aattcatttt cacttgctga atgaacggta cgcttggtcg
     2101 aataaaacac ctgaatattc gggaactttt cctgcatttt ttgataataa taaattagag
     2161 gttgttcttc tgcttctggc gctttctcga tacctaacaa ataaagagca tccatttttc
     2221 cagctgagca gatatctact aaaggtaaaa gctgatggat tacttcgctg gcttcttgct
     2281 gtgtccataa ttttccacga tagttgatat cgaagctgat caggcagcca gctttttttg
     2341 cagcttctat cacctgtttc gtcatttttt gccatttttt agacaaagct ggggtgattc
     2401 ctgaaacatg aaatatatct attccttgga ataactcctc tagcgaccat tccaaatgat
     2461 ccatttcagc aaaacttgat ccggcacgat catagatcac tgaggcggcg cgttcgccac
     2521 taccagtttc catataataa gtacctagtc ttgatccacc agataatacc tgtttcgtat
     2581 ctactccata acattgcaag tgctttttga ctgcttttcc caatgcgtta tcaggtactt
     2641 ttgtagcaaa aatcgcttca tgtccgtaat tagctaaaga aattgctacg tttgcttccc
     2701 ctccgccata atgggcagcg aatcgttcac ttgaatcgat acgggttccc tgctctgtgg
     2761 acaatcgcaa catgatttct ccgagtgtca ctacttttcc cataagcagc tacctcttga
     2821 tttctttcat ttttgctgca tactgttgcg cgacttccgt tactttgtca aaatctcctg
     2881 tagctgcggg tgctaaaagg ttcccaccta ctccgactgt tacgacacca gcatcgaacc
     2941 actctttcat attgtccaga ctcacaccgc cagttggcat gatattgact tgaggcatcg
     3001 gcgccttgaa ggcagaaatg atacttggtc cataagcgct tccaggaaat agtttgacga
     3061 tatctacccc gcttttcagc gctgttttga tctcggtaat agtcatacat cctggtaagt
     3121 aaggaatttg atacagattg cacatctctg ccgtttcttg atcaaaggaa gggctaacaa
     3181 tatactctgc gccagctaag atagccaagc gagctgtgac agcgtctaat actgtgcctg
     3241 cgccaatcac tatttctggt tggtctttgt agaatgacaa aagttctttg attacttgat
     3301 ctgcttgagg gactgtaaaa gtcaactcaa taccagttag accacctttg atgattgcgt
     3361 ggcaagcatt caaagcttct tctttgcttt ttccgcgtac caccgcaatc actccggcgt
     3421 ttttcaatcg agataaaata tcaatttttt tcatcttctc cacctatttc ctatttagaa
     3481 attttatttt ataacttgga aatctatacc catattatac ggcagaaaag aaaaattgca
     3541 aacgcttttt tcattatttt tgaattcttt gctcgatttc gctttttgtt tgcttgaggg
     3601 cactgattat ttgttctttt gtctcatctg ttaaacgata tttcggcata cttacactaa
     3661 atgcaccaat gatttgttcg tttttcacga ttgaggctcc tatacaaaaa atatcacgtt
     3721 cgatttcttc atcgtcaaat gcgatgtggg tttcttttac cgttttgatt tcctttttca
     3781 accgtaatgc attggtgatc gtatattctg tgtaagggat caacggatgc ttttcaagat
     3841 aatcagtgta ctgctcttca ctgaattctg ctaatacggc tttgcccatc gcagaattgt
     3901 ataatggtcg ggtgattccg atttttgaag acatgcggat cgtttgatgt tttggatcta
     3961 gtttgttcac gtacaagatc tcattgttgt ccaatacccc taagtggata gtctcatcga
     4021 tcttttgctg cagctgttct aaaaacggta aagtcaactc taccaaatcg atttgttcaa
     4081 tgtttttatt cgcgtagcga ataagctttg cacctaaacg atagtttttc tctgaatttt
     4141 tatttacgta gccgatcatc aacaaagtat ctagtatttt caagactgta gaagaggtca
     4201 ttcccacacc ctttgcgatc gtctgcaaag agacatcttg gttttccgct aaatagtcta
     4261 gtattttaga ggctttgatc aatacagttc cataaggctt tttattttcc atataatatt
     4321 ctcctatttc tttataagaa aaattatagc ggaaatacca tcaatttaaa agagatatcc
     4381 agatataaac taccatttct ctttacaggc aatttaatgt tgaagatccg cttcgttttt
     4441 acatcaaact ttgatataaa gcaatgattt cttctactgt cgtttctctt ggatttcctg
     4501 gtgtgcatac atctcgcaaa gcatcttctg caattgctgg aatatctgct tcttttacac
     4561 ctaattcaga aatggtttct gggattccaa ccgctttgct caaccgatcg atctcaccac
     4621 aagccgcttc tcttacatct tctaaagaca tttcagctgc tcctttaatc cctagaacaa
     4681 gagcgatctc acggtacttt tctccagtat attctttgtt atatttcatg acagttggca
     4741 aaagtgctgc acacgctacg ccatgaggaa tgttgtacca tgcacttaac gggtgagcca
     4801 tcccgtgtac cagtcctaaa ccgacatttg agaagcccat tcccgcaata tactgtccta
     4861 atgccatttt ttctcgccca ttttgatcgc catctacaga agcttccaaa gaatggccga
     4921 tgatttcgat tgcttttaaa tgcagcatat cagtcatttc ccaagcgcct tttgtgataa
     4981 acccttcaat agcatgcgtc atagcatcca tacctgttga tgcagccaat tttttcggca
     5041 ttcccatcat catatcgctg tcgatgaacg cgacgattgg gatatcatga ggatcaacac
     5101 aaacaaattt tcggtgattt acttcatctg tgatgacgta gttgatcgtt acttctgctg
     5161 ctgttccaga agtcgttggt atagctaata taggtaaaca tggatttttc gtatcagcaa
     5221 caccttctaa actaatgacg tctgaaaact ctggattcgt caaaataatc ccaatggctt
     5281 ttgccgtatc gattggcgaa cctccaccga ttgcgatcaa acagtctgcc tctgcctctt
     5341 taacaaaagc cactccatct tttacatttt ggatcgttgg attaggtacg atcccgtcgt
     5401 ataaagcata agcgattgct gctttatcta gaagttctgt tacttttgtt gctataccgt
     5461 gctcgatcaa tcctttgtca gtaatgacag ctgctttgcc gaatcctcgc tgctgaaatt
     5521 cagggacgat cgactcaatc gctccttttc caaaatacga tgtttcattt aaaatcattc
     5581 gattactcat tttctattcc tcctaatttg tattattttt cacaataaaa acgaaagaac
     5641 cccaagcaac cggttgccca gttgcttgtt gctacagttc aaagactggc atcaattccc
     5701 aactgactgg agaggcatct ggatttgtct ccatgatatc ttccatgtac ttccaccatt
     5761 tttgattgat agatgtattt gccatttttt cccataactt ttcatcttcg atcactaagt
     5821 aaccaaataa caaacttgta ctcggatcaa gatagatcga atagtcgatg accccgtgtt
     5881 cacgaagtgc tgtacgcatt tctggccaaa gttcatcatg tctttttttg tattcttctt
     5941 gtttttctgg gtaaactttc atgcaaaaag cttttttcac catgtcagac acctgctttc
     6001 gtatgaactt cctccaaata acctgctttt ggagtgacac caaacgcatc tgctaaatgc
     6061 cacaggtttt cgtccgtgat tgtttgcaag attggtcctt gcgctttgac gtacgtatag
     6121 acttctgctg ccttttctgc tgtctcgatc aaaccaaaga cttcatccat gtcttttcct
     6181 gacccgtaga ttccatgttg tggccataaa accaatcgag tttctttcat tttttcagct
     6241 gttgcttccc caatctcatt tgttcccgga accatccacg gaatgatgga aattccttct
     6301 ggaaaaacaa ctaagcattc cgtacacatt tgccatagcg tacgactaaa ggaacgttcg
     6361 tctaattcat gcgtaaaact catcgctaat agatgactag catggcaatg cataacgatc
     6421 cgattatctg ggtctacttc taaacgggcg atatgactca tgaaatgact tggcaactcg
     6481 cttgtaggaa ggcattcgtt ttccagcccc cacaaaagtt ccagtgtttt gccatcttca
     6541 gcaactcgaa cgattccaag attctcggca ggttcgctcg ctacattttt aaaatatctt
     6601 cctgaaccag ttacaataaa atagcatcct gctaaacggc tggcatcaaa aatcatcgag
     6661 atgcggcgga tcactttttc aggatccaag aacggtgtga tctcttcttc ttttagtaaa
     6721 taagaaatat tcccgccatt tctttcatcc catcccaagc gataaagatt cgacgtcgtt
     6781 tcgatcattt ctttcacata aggcgcttgt aaaatatcta tttttttcaa tatagccaca
     6841 ctcctatttt cttaattcca acacgtcttt ttgatattcc tggacttctt ccagccaatc
     6901 caaaccaact ggaacttgat ttttgtaaca gaaataattc catacatcgg catacgggaa
     6961 atctttcaat tcttccgtat aagccattct tgaagtaaag tcaaaagcca gctccatttc
     7021 tttcaatcgc tcaactggtt ccagcatagc tttcagcagt gctttttgtg tatttctcgt
     7081 accgatgacc caagcagcga tacggttgat cgttgcatca aagaaatcca gaccgatatt
     7141 tgttttttcc aacaattcgt tacggactaa ttctttagcg atttcctgca attcatcatc
     7201 catcaaaacc acatggtctg aatcccaacg cactggacgg gaaacatgca acatgattcc
     7261 tttgctgaat aaagctaatg aagataattt attggaaatc acttctgttg gatgaaaatg
     7321 tccagcatct agacaaacaa gtttgtcacg agtcaacccg taacccatat aaaattcatg
     7381 ggaaccgact gtataagctt cagcgcctaa accaaatagt ttgctttcta ctgcatcttg
     7441 tacataccgt tcatctaact cttctgtaaa aatttcatcg agtgattcca ttaaacgttc
     7501 ccgtggggta aaacgatcaa tcggattatc tttcatccca tcaggtaccc aaaagttatt
     7561 gatacttgtt tgcccagttt ctttcccaaa gtactccgcg attttgcggc ttcttttccc
     7621 gtgttcgatc cagtattcac gaacttcttt atctggatga gccaatgtaa agccgtcttt
     7681 catcattgga tgcgaaaaga aagtcggatt gaagtcaaga ccgatttctt ctttttttgc
     7741 ccactctacc catttttcaa aatgtttcgg ctcaatttca tttagatcga cagattcttc
     7801 tgtatccaaa tagatagcat gaaggttcaa tttgtgtttg cccggtatca acgaataagc
     7861 cttctccaga tcttgtctaa gctcttcagg tgttcgtgcg gctcctggaa aattccctgt
     7921 cgccatgatt ccaccggtca attcgccatc cggtgtcaag aaacctttga catcatctcc
     7981 ttgccagcaa tgcatagaaa ttttgatgtc ctgtatcttt tctagtacag catctgtatc
     8041 tacaccgata gttgcatatt tttcttttgc ttgttcgtaa tttctttcaa tcgtgttcat
     8101 acgattactc ctttgatttt attcacgtac cattccatcg tgttttgctt attttcatct
     8161 gggtaaaaag tttcacatgg aaacgactgt ttgattacat ttcttgcttg ttctatattc
     8221 gaaaactgac ctgtactgag catttgcatg gcaacattcc cgattgctgt ggcttcacta
     8281 ggtcctgctt ctacaatgat tccggcataa tctgctgtca attgattcag tagacgatta
     8341 ttcgatccgc cccctacgat gtgcagcttt tccaatttcg ttccagttaa gtgttctact
     8401 ttgatcagct cttgtgcgta acaaagagca agattatcat acacacaacg agcgatagct
     8461 cccgatgaga cagggactgg ctgctctgtt tcaaaacagt attgctgaat ctcttctatc
     8521 atatttgggg gattcaaaaa tcgagggtca ttgatttcca tcacttgctg cagcggtttt
     8581 tcttttgcag caagctctgc caattccgca taactgtact cgtactcttg cattcgggca
     8641 acttcctgga tcaaccacat ccccatgatg tttttcaaaa aacgcaccgt gttatgggca
     8701 ccccattcat tcgtataatt ttcagaaaaa gcattgcgag aaacattggc gatctttgtt
     8761 tcaatcccta aaagcgacca tgtcccactg ctgatataac cccaacgttc tccttgacct
     8821 ggcgttccta atacagctga cgccgtatca tgacttgcta ctgtgataaa ggtcacatct
     8881 ggcaaatcat attttgaaaa ttgttctttt tgcagatctc ctagtatcgt tcccggctct
     8941 gtcaatggtg gaaataattc cttcgagaca ccgatttttt caagaagttc tatttcccat
     9001 tcttttgtcc ctgcattcag cagctgcata gtagatgcat tagttttctc cgtcaccatg
     9061 ttaccagtaa agacataccc taaataatca ggtatcagta acaatttatg tgcttttgcc
     9121 aaattttctt tttcttcagc aaacaattgg aacaaggtat tgaaaggctg gatctgaatc
     9181 cctgtctgct catacaactt atctagagaa tagatttcag aaaactgttc tactgctcca
     9241 ttggtacgct tgtcccgata agcaatcgga tcatttatcc gatccccttg ttcatcaatc
     9301 agacagtaat cgactgccca tgtatcgatc cccgcaaaaa ggcgatcaat ccctgtctgc
     9361 tttaattttt caaatccaat caaaatctct ttgataagat aatcgatctt ccagcgatcg
     9421 tgaccgtctt tgttccgaaa gccgtttttg aaccggtgta cttcttccaa gatcaaagaa
     9481 ccttttgcat ccctcgtact tttcattagt cgtccgcttg atgcaccgat atcgatggca
     9541 acaaatgttg gattcatttc tccacctctt ttactttatt gttgattagc actttattaa
     9601 taaagcgtta tcatttattg acaatgctat cataatacag aatcttatat tttgcttcac
     9661 actattttgg tatataattg cattatcttg acaagttggg agtgtttaca tggattactg
     9721 ggaaaaagcc aaccactcat ggtcggaaga ctcaaaaaga gtgatcttga cgcctacaca
     9781 aagaagcagg aatttatttt tttatatcca agaaatcggt tacttcaaag caccaatccc
     9841 ttactatact gaacgagcac atttaccttc ttacttgata aagtttactt tgtctggcaa
     9901 aggttcgctt acatataatg ataatcagca acagctaaaa gctggagact tattttttat
     9961 tgactgccaa gaatatcagc gttatcaaac gatcagcgaa gaaccatggg aaatggattg
    10021 gatccatttg aatggtcaga acgtccaatc attttatcag gagttcacaa aagacggcca
    10081 taacaccttc cattcaacag gagatccatc agaaaacaag atccaccatt tgatcacaga
    10141 attgatccac atgcaacaag gcacgaatgc ccgtaccgat ttccagtcct ctgtagctat
    10201 ccacgagttg ctgaatgaac tgattttgca aaagtatcag ctagatttcg aagaagagga
    10261 tattcccgcc tacgtttaca aactgaagca atacttagac gaacatttca aagaaacgat
    10321 tacacttgat caattagaac aacaattctt attgaataaa tatcagctga gcaaagattt
    10381 ttccaaattt atcggttctc caccaatcga ttatttgatc agcaaaaaac tttcttacgc
    10441 taaagacctt cttcggtata cagaatttac ggttcgaatg atttccgaag aaatagggat
    10501 tgagaatttt gcttacttta gccgcctgtt caaagcaaaa acaggactat cgccgagaga
    10561 ctttcgtaaa ttcagctgat tcatataata tgtacgtcat tttttcaaaa tattttgaaa
    10621 tatatagata aaaataaaat aaccgcagtt tctgcttcta tgtaaacaac agcagctact
    10681 gcggttattt tttagtgatc attctcattg ttcatcttca aaatgttcat aatgaaccaa
    10741 ctgtttctgt gtatcacgtt tcaaaaattc tttaggcgtt atgccgaact ctctcttgaa
    10801 tgctttcaga aaatgcggat aagaagaaaa accactttta ctatatacag cactagagct
    10861 gcggtattct cgcaacaagt gcttggcata gatcagcttt ttcttcatta catactgatg
    10921 aaacgtaaat ccagtgtact ttttaaattc tcgtgtgata tggtatttgc tgacaaaaaa
    10981 ctgcttttcc atttcctcta aagaaagcgg atgagataaa ttttttgaga tatattgtaa
    11041 catttgttcc acacgctcat taggaactac agcattctgt tcttgcgcac tctcttcttt
    11101 ttgtacaact tgattgagaa aaatcaagta ttctaccaga gcttgttcat attttatgtc
    11161 ttcaccgtaa atttttttat caggaggatg gtcgataaat gataattttt gcttcaatac
    11221 ttcctgttct atttgcaata cacgactcct cttctctccc attggttgaa aacaagccga
    11281 tagattagac catggcgtgg aacgactttc tagaaaagaa ggggtgacaa agatatagac
    11341 acgctcgaat acatctgaag attggcggac gatacgatgc aaatcattgg aatggatgag
    11401 cagcacactt cctgctttga tcgtaaattg tcgtccgttc aaataaaaag tcgcttctcc
    11461 ctttaatgta gcatggatct catagaaatc atgatgatga tagatcgttt tattttggct
    11521 gacgacatct ttgacatgat agatttcata ctgatcagaa accatcgtat tgacttgttc
    11581 accgctgatt ttcttttgga gcatgtgccg cacctccttt tgttcactta tgcacaattt
    11641 tattatacca cgttttttgt aatcaaaaag agctgctttt atatactctt ataaaaaaga
    11701 agagacgtgg catcttctag ccccgccccc tccattctaa atattagtta accaacgtct
    11761 tctttacatt attcaagaag acttcttcca taacgaatcc catcatcttt tgtttcttca
    11821 agaaccacat acaggtacgg acgattcttc atgatgattt ctttgatttt tgtgtactcg
    11881 atcacaccgt ctcccaaatt taccggcacg attttttcct gttcgacacg aaaatctttc
    11941 aaatgaaccg cacatatctt ttcaccaaaa cgttcaaaag cttcttctac caattggact
    12001 tgttcttgat aatcactact tgtgatcaaa ttggtcggat ctaaaatgat tcctagatag
    12061 tccgaatcta ctgcttggat cagctgctct acccttgcta aagaatacac cggatgattc
    12121 aaacctggtt ctatccccac catcatttgg tatttttctc cagcttttac caagcgacgg
    12181 ataacggaca cagcttcagc aaatgcttca tctgtgaaat tttcttccgt atattggatt
    12241 tccggcaaaa cacatccagt ttccgaagca accatcgacg caccaaagga agaagcatag
    12301 cgaagatact gctcgaactt tgtcagcaac tcttgtcgta tcgttaaatc tggatgaatc
    12361 atattgatat agcaacttaa aattcctacg gtcacttctt gcttttctaa aactcgtttg
    12421 acataatttc ccatcccaga attgatctca tttgcgccag aaggtatttc aggaaacgaa
    12481 agacctaagg cgagctgcag tgtatgaatc ccttgttctt ttgttttttc tgcaagatct
    12541 tctatcgttt ggacttcgga aagatcatgt cctctaattg taatgttcaa cataactatt
    12601 ctcctaactc agcatattgg gtagccacat gacgatttgc gggaagtaag taatcaaaaa
    12661 caagacgact aagataaccc cataaaaagg aagaagcggt ttcagcattg cctctgcttt
    12721 tacgcctcct acacttgcac cgacatatag tcctgtacct actggcggcg tgatggagcc
    12781 tacacacaga ttcatgattg aaaacaaccc gtaatgtaca ggatcaaccc ctacacttgt
    12841 gacgattggt aaaaatattg gtgtaaaaat caagatagca ggcgccacat ccataaacat
    12901 acccaccagt aataacacaa gattgactaa taacagaata ataaatttat tatccgttac
    12961 gcccataatc aacgaactaa ttgcctgagg aataccggta aacgccatgg cgaaagacat
    13021 catggaagaa gtagccaata agaacatgat cgtccctgac attgctactg attgaaacaa
    13081 catattaggt atatctttta gttttaccgt tttatagtaa aacatagcaa tcaacaggga
    13141 gtaaatgaca gctatcgctg aagcttctac tgctgtaaat atccccgtca agataccacc
    13201 gataatgatg acaatcaata gaaggctagg aatcgcctct tttattattt ttccagcatg
    13261 agccatctgt cctttttcga caacagggta tttattcttt ttagcaacga tgaatgctac
    13321 tagcatgatt cctagcgccc acaaagcgcc tacgagataa cctcccataa acaaagcaga
    13381 gatcgaagca ccaccagcaa caagtgaata catgataaaa gcagtacttg gaggaatgac
    13441 catacctgtc ggagctgacg cgatattgac cgctgtcgca aacttccggt catatccttc
    13501 ttccacttgc tgagggatca acactccgcc aatcgcggta gaagcggcaa tggccgagct
    13561 agagatggaa ccaaataaag cattccctaa cacattcgta tgagccaaag cccccggcac
    13621 gcgcccgacg aacaacatcg caaaatccac tagtttctta gctatccctc cattattcat
    13681 gataatgccc gagagaacga aaaacggaat agcgatcagc gaaaaactat tgatacttcc
    13741 caccattttt tgagctgaac taaaagcaga aacatcaaac ggtataacta acaataaagt
    13801 catcatcgat gagaatgcaa tacttatagc aatcggcatc cctaatacaa gcaacacaac
    13861 aaatacaaaa aataacacta atccggcttc tattgccatt ttattcctcc atttttatat
    13921 tttttcttga attgctggct tgttgcttaa tgtatcgatt agattgatca ggctatagat
    13981 aatgatgagt acccccgata caggtactga cagataaaca tatcccatag gaatacttaa
    14041 agaaggcgag atctgcccca ttgtcaatgt gacagcattc catccgccat acaccatgat
    14101 catcgcagca aataccagaa ataaactttg aacaacaact tctaaaattt tacttttttt
    14161 gccagttaat ttttcactta aaaccgttac ggctaaatgt gctttttgtc ccacaacata
    14221 cgcggaagac aacatggcaa accagacaag accgaatcga acaagctctt ccgtgaccgt
    14281 atttggattt cctaaaaccg ttctggaaaa tacttgccat aaaacaacag cgaccatccc
    14341 aacaagcaaa acagctccca ggatctctaa taccttgttt aaaatcattt tcatcacagc
    14401 tacgctcctt gctcttggat cttttgataa aggtctttgt aggtaggatc gtttttgaat
    14461 atatcatgaa gtggggcaac tgcctctcga aacggtgtat tatctacatc attgaattgg
    14521 actccatgtt cttcttgtgc taaagctttt tcttcttcaa ttgcttttgc aaaagcttca
    14581 atttcaaaaa ccgttgattc tttcgctgct tcttcaatgg ctttttcctg ctcatcagtt
    14641 agctgttctt tgatggcatc attcatgatg atgatatctg gcacacgtgt atgctcatca
    14701 taggaatagt atttagccac accaccatgt ccagctgtat acaagacaaa ttcattgttt
    14761 tctgatccgt tgatcaaatt ggattgaaga gacgtgtaca cttcatcact tcccataggt
    14821 acgggcgaac cgcctaaaag ctctaccatt ttgatagcgg tttcgctttg catcacacgg
    14881 atcttctttc ccttcaaatc ttccggcgta tggatcggac cgtctaccta taaaagctgc
    14941 gctgaccaga atcataatag gtcaatccgg taaatcctaa atttttggtc gattggtaga
    15001 ttggctccat tatttcttca ttatccatca ccttaaagaa atgttcttca tcattgaata
    15061 gataaggaac tgaaaaaatc gaataatctt tagagaaact ttccaacgct gaggcactta
    15121 cttttgtgaa atctactgct cctgactgtg tcaattcgat cagttcacgt tctccaccca
    15181 gttgtccatc tgggaaatac tggatctgta cggaaccatc tgttttttct tttactaaac
    15241 gcccaaattc attcattgca tcaattactg gctggctgtt gcttgcataa gcaaagcgca
    15301 aggtaactat ctcatttaca tctgcatctg cgacagttgc cacactagaa ttatttccgc
    15361 agccgcttag acctagcaga cctaggctaa gcgaacttgc tgcaaacagc agtgatgttt
    15421 ttttcatatg ggtttccttt cttttaacaa taagagacga gcaattctgt caaacaaagc
    15481 accgttaacg actgtccata aggcattgct gtgattccga tattccgata aaaatctaaa
    15541 ctgtccccca ttcctgtccc gattgaaacg ttttgaactt ctcctttttc atcgatttgt
    15601 tcaagcaatc cttggatagc acgataagcg acttctttat attcttgtgg taaataacgt
    15661 ttatggaccg ctttcaaaat accgtaagca aatcctgcag ttgcagaaga ttctacgtaa
    15721 gaagaaggat catccaacaa cgtatgccat aaaccgcttt cagactgata agtttttaat
    15781 gcacgcactt gtgcttcaag tgtttcgatc aataatttgc gcaatgcatc ttctttcgat
    15841 aattctaaga tttcaatgat ctctggaata gcaatcgtca accagcagtt tccacgagcc
    15901 caaaatgctt cggcatagtt atgatttcct tcaaacgtcc agccgtgata ccacaatcct
    15961 gttcgtttat ccatcaagta tttgatatgg atcataaact ggtaacgagc ttcttctaaa
    16021 tattctgggc gattcaacag tttcccgatt tttgccaaag gaagcactgt catcatcaat
    16081 gtatcgtccc acaactgatt cttattttcc ggtccatatg tggcatgctg taacccgtct
    16141 tcatttgtcc gaggcatgtc attcatgacc cattctgccc attgttctaa ataaggcaaa
    16201 tagcgtgaat ctttcgtgtc ttcatacaag taagccatag tcaatagcgg tgccattgta
    16261 ttgacgtttt tgggcggagc gccttcttcc atacgatcct caaaccattc attgaccact
    16321 tgataagcgc gaggattttt tgtcatctgg taatttttat aaataccata tagaccgaca
    16381 ccttgtggcc aattccacac tgtccagctt ttgtcatcta ctttcagtcc atcaaagtcc
    16441 aacaaaaatt caccattatc gtctttgatc gttgtcaggt tttcaacgag caggtcaatt
    16501 ttttctttca ctaattcttt aggtacaaaa atctcttctt taataatcat ctttacgcca
    16561 cctttttaaa cgctttcatt ttatatcctt aatatagcag taataccgtc cactataaat
    16621 atcgcttttt gaacaaaaac tgtctaaaat tgacctaaat gttaaataac aaaagtaact
    16681 ttcagttact tggaaattaa aaaaagaact tggagaaaca acgttttcca agttcttttt
    16741 catctatttt cttatctttt ttctacgctt tcttttgctt atccgttgta agcatcaatg
    16801 atgatctgtt tcaattcaga gatcaatggt tctttcggat tagctgtcgt acattggtct
    16861 tcgtaagcta attcagccat acggtctacg gttgtatcta gtgtttcttg tgtgactcct
    16921 tgagctttta gactcatgtt gatgccgaca gattttccta attcataaac agccgttgct
    16981 aaagcttcga ctaattcctc tgttgtttcg cctttcagac ctaagaattt tgcgatatct
    17041 gcgtaatctg tatctgcacg gaaatagtca tatttaggga acatcgcatg tttttgtgga
    17101 tcttttgcat tatagcggat aatatgcggt aaaagaatcg catttgtccg tccatgaggg
    17161 atcccgtatt ccccgccgat tttatgtgcg actgagtgac aaatacctag gaatgcgtta
    17221 gcaaacgcca taccagccat tgtagaagcg ttgtgcattt tttcacgtga ttccatatca
    17281 ggtgttttta ctgatttttc taaatagtca aagactaact tgattgcttg taggctcaat
    17341 ccacgtgtat aatctgaagc catgactgaa acataggatt cgattgcatg tgttaacaca
    17401 tccatcccgg tatctgctgt gacagatgct ggaactgaca tgacgaattg aggatcgacg
    17461 attgcgacat ctggcgtcaa tgcataatct gctaacggat atttcacatg tgtttcactg
    17521 tcagtgatga ccgcaaatgg cgttacttca gaaccagtcc ctgatgttgt cgggatacat
    17581 acgaattgtg ttttttctgg tttgtcgatc ttatatgtcc gtttacggat atccaagaat
    17641 ttttgttttg caccaaagaa agatgtgtct gggtgttcat agaacatcca cattccctta
    17701 gctgcgtcca ttgcagaacc gcctccaaga gcgattactg tatctggctt gaagtctgcc
    17761 atcagtttcg ttcctgcgta tacggtattt gtcgatgggt tcggttcaac atctgagaag
    17821 acttcgatct tcacatcatt tttacgtcgt gccaatactt cacgaactgt atcgcagtaa
    17881 ccgaattgga ccataccagg gtcacaaacg atcatgacac gttcaacgtt ttccattttc
    17941 tctaagtaca atagagagtt cttttcaaag aagatttttg gcggtaattt gaaccattgc
    18001 atgttatttc tccgtttcgc tacagttttg acattgatta agtttacagc agagacgtta
    18061 cgagaaacag agttcttacc ataagaaccg catcccaaag tcaatgacgg aatcatttcg
    18121 ttatagatat caccgatgcc gccttctgca gatggtgtat tcactaaaat acgacaagct
    18181 ttcattcgaa gaccgaattt gacatgcaac tcttcgtttt ctgaatggat aactgctgta
    18241 tgtcctaatc ctccaagatt caacatacct tcacacaaat caaacgcatg gtccgtgttg
    18301 ttagctttga tcatggcaag aactggagaa agtttttctc ttgatagcgg ataatctggg
    18361 cctacgcctt ccaattcagc aatcagcatt ttcgttcctt ttggtacact gattcctgcc
    18421 aattttgcga tttccatcgc agaatgtcca acgattgatg gattgaccgc atattttcct
    18481 tcgttcatca ctgcatcttc taatttttgc aattcatctg gttttacgat ataaacttgg
    18541 tgtgcttgga attctgcttt tactgctgca tagatttctt tatccacgat cacagcttgt
    18601 tcagaagcac aaatcattcc attatcaaat gttttcgaaa cgatcaaatc gttcactgca
    18661 cgtttaattt ttgctgtttt ttcaatataa gctggtacgt tacctggacc tacacctaaa
    18721 gctggttttc ctgtagagta agcagacttg accattccag ctccacctgt cgctaagacg
    18781 atcgcgatac ctggatggtt cattagcata gaagtcgctt cgatcgaagg atgttcgatc
    18841 cattggatac aattttcagg agctccggca gcgatagcag catcacgtac cactcgtgct
    18901 gcttctgcag aagatttttg ggcacttgga tggaaagcaa aaacgattgg atttcttgtt
    18961 ttcaacgcga tcattgattt gaaaatcgtt gtggaagtcg gattcgttgt tggagttact
    19021 ccgcaaacca caccgactgg ttcagcaatt tcaatcaacc ctttttggac atcttcattg
    19081 atcacaccaa ctgttttatc atgtttgata ttatgccaaa tatattcgga cgcatacata
    19141 tttttgattg ctttatcttc gtaaacaccg cggcctgttt cctctacagc catcttcgct
    19201 aatggcatat gttgatccaa agctgccatt gccatttcat gcacgatatg gtctactttt
    19261 tcttgatcaa agctttccat ttctttcaat gctgcatttg ctttttctgc caaatcatca
    19321 atcattgctt gtacgtcaat tgtcttcgtt ttttcttttt ctattacttt agccatcatt
    19381 atcctcctaa tgggtcatgg ttatttgtta aataattcac atttatatca tacaatattt
    19441 atttttctat gtaaacactt ttttgtgatt tttttcacag ttatttttaa atataaaatt
    19501 gctttattta caggtgtttg tatagaaaaa tatataaagt tatttattat tatttatatg
    19561 acacacccga ttattcaata ttgagcaata tatcccatgc tcttatctct ttcttttttg
    19621 aataaaaaat gaggtaggac atctttctgc cctacctcgt ctttaaataa gctttgatac
    19681 ttatttttct tccttctctt ctgtctcttc cagcgtttct gttgtttcag tttttacttc
    19741 aactgtctca gctggttctt ctgttgtttc aacagtagtc gttgcatcat ttgttacgac
    19801 agtccctggt ttgaccgttt tgattgctgc acgatcgtac tcaaggaaga tcccttcgca
    19861 gtctaaaaca actgtccgtt tgtcattgtc gatttctgaa acaactccat gtaatccgcc
    19921 gatcgttacg actgaatcac ctggtttcat gctatctaac aagttttgac gttcttgctg
    19981 ttgtttcttt tgcgaacgtg acataaaaaa ccacattgct aatagcgcga taaacatcac
    20041 aagcattggt aatgttccca tttttttcac ctcactaatt ctgtatctac aaaaaacact
    20101 ttagcagaaa aatgctgata acactagttt tttacttaat ttttaaaaga ttttccttta
    20161 gaagcttttt gcgttttctt tattgaaccc gtattcttca aaaaaggctt ctctaaattc
    20221 aagcaaatta tcatccatga ttgcttgacg tacttgtttc attagattca acaagaagta
    20281 taaattgtgg taagaagtca aacgaatccc aaatgtttca tcacatttga tcaaatggcg
    20341 gatataggca cgtgtatagt ttttacatgt ataacaatca catttctcat caattggacg
    20401 gaaatcatgg gcatatttcg catttttcac tactagacgg ccttgagaag tcatacatgt
    20461 gccatttcga gcgattcgag taggcaagac acagtcaaac atgtctactc cacggataac
    20521 accatcgatc aatgaatcag cagcaccgac acccatcaag taacgtggtt tgttttcggg
    20581 gatcaacggt gtagtgaatt ctaatacacg attcatttct gatttaggtt ccccgacaga
    20641 taacccgccg attgagtaac cagggaaatc catcccgact agatctttcg cgctttgacg
    20701 acgaagatct tcaaaccctg ctccttggat gattccaaat aatccttgac ggtctggatt
    20761 agcatgagct ttcaatcctc gttctgccca tcgagacgta cgttctactg attttttcac
    20821 gtagtcgtaa ctttcatcga atggtggaca ttcgtcaaaa ctcatcatga tatcagagcc
    20881 caatttattt tggatgttga ttgctttttc tggtgataaa aacatacgcg aaccgttcaa
    20941 gtgatttttg aaatgcacgc cttcttcttc gatattccgc atatcactta atgaaaaaac
    21001 ttgaaagccg cctgaatctg ttaaaatcgg ttgatcccag ttcataaatt tatgcagtcc
    21061 gccagcttct tctactaagt cttcaccagg gcgtagccat aaatgatatg tattacttaa
    21121 aataacgcca gcacccattt ctttcaactc ttctggggac attgttttta ctgtagctaa
    21181 tgttcctact ggcatgaaca ttggtgtggg gaaagttccg tgtggcgtga tcaattcccc
    21241 taaacgcgcg ccagtatgtt tttctttctt gattaaacga tagcgaatgg caggttgcgt
    21301 cattcatttc cctactttct atataaaata tcttgacaac tcccaccatc ataatcgttt
    21361 caagtgcttt ttgcaagttc cctcggcttc cttctacttt atttaatatt ctttttttta
    21421 tccttttttt atgaataaca aaaaaaagaa aaacattatg acagaaatat tttttgatac
    21481 atggtatatt ggtgttatta aagaaaagga gggaaaaatc atgcaatctt ctgcagaatt
    21541 aaaacgccaa gcaaaagaga gtctaaaagg acgttgggga caagctgttt tactaaatct
    21601 aatccctaca ttgattggta ttgctactat tcttcttatt atgattccta ttatcttgtt
    21661 tgccaccacc atatatgatg gatcagcgag cgtctcagaa gcgacaactt caggaggcgg
    21721 tagttcagga ggcggtggcg gaattatttc cagcatcatt agcgctttgt tcatgagcgg
    21781 tatttcttgg acttatcttg atattttacg tggacaaaaa gatcagatcg aaccattcaa
    21841 agatgcattt cgtggattca aaggtttgtt tatcggcgga gtgatcagct tagctgtgct
    21901 gatctcgatc tttacaacat tatggggttt gctattagtt attcctggaa tcattaaaag
    21961 ctattcttat tctcaaagct attttattta ctacgatatc gtccaagaaa caggacaaac
    22021 acctaaagta ctggatacga tcactgcaag tcgaaaattg atggatggtt ataaaggaca
    22081 acttttctgg ttagatcttt catttatcgg ctggcatatc ttagcattag caactgctgg
    22141 tattggttat ctatggctca gcccatacat ttctgcaaca aaagcagcat tttatgaaca
    22201 attgccaaaa gatatttaaa agagagtgtg acttgtgacc gagggggtgg caggagaaca
    22261 ccgtttatcg agaggcagta aaagaaacat tcagcccatt agcaaaaagg aggtgtgaca
    22321 acattcatgt cacacctcct ttttgtatag ttccagtaaa aagaaaatca acttagaaac
    22381 agttaatcat tcctcttctt ctgcttccgg taatcgatgg aacttgatca gttcattcac
    22441 cgcgattggt acgatagata aaatccctac caagaaccag tgattcaaac tgatgtgtac
    22501 taggccgaat agttcttgcg taaatggcag caaagcaaca gccgttgtaa tagctaatgc
    22561 cagtaacgcc atttctacca aagctttatt ggtcgctaaa ttgactttga agatcgattg
    22621 gctgctgcgt acattgaaca catgcagaat cgatgaataa gctaagatca agaatgcaac
    22681 agtttgaccg acatgctgac ttgcgtctac ccatgggctg atagtatcga cataactacc
    22741 taaataataa ccaaacaagg ttacgatggt gaagacccct gcatttatgg caattttttg
    22801 caataaacct cgagcaaaga tcccttcatt tttcggaatg ggtggctgat ccatgatgcc
    22861 gtgttccgct ggctctcttc ctaaagcaaa tcctggaagt ccatcagcaa ctacattgac
    22921 aaacagtagt tgcacagctg taaatggtgc tccccatcct aacagcatcg caatcaaaac
    22981 aataaagatc tccgaaatat tacagcttag caaaaagttg atagctttac gaatattctg
    23041 ataaaccgaa cgtccttgac tgacagcgtc tacgatcgtc gcaaagttgt catccgtcaa
    23101 aatcatatct gcggcacttt gcgccacatc tgttcctgtg atccccatgg cacacccaac
    23161 gttacttgct ttcaacgctg gtgcatcatt cacaccatcc ccagtcatcg ctacgactgc
    23221 acccgtacgt tgccaagatt tgacgatccg gatcttgtct tctggcgtta cccgagcata
    23281 gacagagaga gctttgacac gcgcatctaa ttcttcgtct gataattgat gcagttctga
    23341 tcctgaaagt gcttctgacg gatcattcaa gatacctaat tctttcgcta tggcactggc
    23401 agtcaccaca tgatctccag tgatcatgac tgttttgatg ccagcctttt tcgctcgctt
    23461 gattgctcct ttactttctg gacgtggtgg atcgatcatt ccgatcagac caatcaaacg
    23521 taagtctttt tccagtgctt cagaagaaat atcttgcggt tcttcgtcat aagtagcata
    23581 accaacagca atcactctca gtgcccgttt accaaagcga tcattaacga tcgccgcttg
    23641 ttctacatcg cctgaactaa agcgcggagc aagaacatcg aatgctcctt tggtgattga
    23701 gatgtatttt tcgcccattt ggtgaactgt tgtcatcatt tttcgttcag aatcaaaagg
    23761 aagctcgttt actcgtggat atttttcttc taattctgcc tttgtatgat aattctcttc
    23821 aacagccctt acgatggcta cttctgttgg attacctttg ataacaaggt cgtcatcttc
    23881 ttgctcgacc gtcacatctg tacatagtgc agccattttc aatatttcca tcgcgctgtt
    23941 cgtcattgaa tcttcgatat tcgttacttc atcttctttg ctccataccc ggcggacgcg
    24001 catttcattt tgtgtcagcg ttcctgtttt gtctgagcag ataacattag cggttcctag
    24061 cgtttcaact gctggcaact gtcggatgat cgcatgtttc ctcgccatct tctgcacacc
    24121 gtatgcaagt gtcaacgtaa caataacagt caaggtttcc ggtacagctg caacagccaa
    24181 ggaaatcgaa gtcatgaaca tctcaaggag tggttctcct tgcagttcac caatcacaaa
    24241 tactaaagcc gcagccgcta aagcgatcat actgattcgt ttgcctagct gattcaatcg
    24301 gacctgtaaa ggtgtttttt gcatagtatt ttcgttcaaa agatccgcga tcttacccat
    24361 ttctgtagcc atgccaatgg cagtgacgat cgctttccca cgacctgcag caaccgtaca
    24421 tcctttaaat accatattaa gttggtcacc taatgactca tcttcttcag caataaatgc
    24481 tgcatctttt tcaactgctt cactttctcc agtcaacgcc gattcttcta cacgtaattg
    24541 tgatgcttcg atcaaacggg catcggcaga aatagcatca ccggactcta aaacaagaat
    24601 atcacccact accagttgct ctgactcaac agtggtcact ttgccgtctc gtatgacagt
    24661 tgccgtttgt ttattcatat cttgcaagga ttccagtgct ttttctgcgt ttccttcttg
    24721 gacaatcgcc aataccgagt tgatcacaac gatagcgatg atcagcagtc cttcaaataa
    24781 atccccatgt tctgtagcaa atgcggtata aaaggaaatc accgctgcta caagaaggat
    24841 gatcgttgta aaatcggaca agcttcgaaa gaattttttg atcatcgatt ctttcggcgc
    24901 ctcatcgaat ttattcaatc cctgcttttt tcgccgttca tccacttctt gtgaagacaa
    24961 cccagtatct ggatctgctt ctgttacctt ctttacttct tctattgtct gcttgtattc
    25021 ttccatccta ttcctccttt aaaatcttcg tacaaaaaaa ggtacttatt ttactataac
    25081 atcctttttt tatttacttc aaataaaggg cttatcgatt atttgttaag aaagcgtaaa
    25141 atttaaagtg cttatatttg caaacgctac caaaggatgc tattatatag atgtaaggaa
    25201 ggaggatacc atcaaataga cagaagaatt tctcgataga acagtgtaag caaacaaagt
    25261 tgttagaagt acaggtttca cccagacatg cgtgtagagt acaaacctat aaatagtgaa
    25321 tgcgtatgga tgtttggatc acgaagtatc cgaaacaaga agtaatgcga tacgtaacaa
    25381 ttgaataacg taaacgtctg aaaattgaat aggaacaaga gatgtttcaa atgttgaaga
    25441 tgtaacgaaa acaaaatttt tgtttaacat gaagaaattc aacaccgtgc tttgcatgta
    25501 tgatggaaag ttacaatcat ttctgtttat ttgaatcctt ccgaggtaca agaaagccga
    25561 gcttatcgag agctcggctt tttctttgtc ataaaatctt tgtaaagctt ttttgttatt
    25621 caaaactagc ggcttttcat aaccttattt cacgaacatc gcatctccaa aactaaagaa
    25681 acgatagcgc tcttcaatcg catgctggta agcagataaa gtcaattctc ttcctgcaaa
    25741 ggcactaact aacatgacta aagtcgattt tggcaaatgg aaattagttg agaaagcttc
    25801 taccactttg aattcatagc caggcgtaat gaaaatatca gtccatccgc tgtctgcttt
    25861 gatttctcca ttaaatttcg ttccaatcgt ttctaatgtt cgaattgaag ttgttccgac
    25921 agccacgatt ttcccgccat ttttacgtac ttggttcaat cgctcagccg cttcttccgt
    25981 caaacggtag aattcactat gcatatggtg ttcttcaatt ttatctacgc tgacaggtcg
    26041 gaatgtacct aaccctacat gtagtgtcag atagactaat tcaacaccct ttgcttcgat
    26101 ctcatcaagt aattctttcg taaaatgcaa tccagctgtt ggcgctgcag cagaaccatt
    26161 ttctttcgca tagacagttt gatagcgatc tgggtcttcc agacgttctt tgatatatgg
    26221 aggcaatggc atttctccca atgattcaag aatctctaga aaaattcctt catactcaaa
    26281 gtcaatgatc cgtccgccgt gttccaattc ttctttcact actgcttgca atctgccatc
    26341 accaaaagag attcttgtcc caactttggc acgtttcgca ggtttgatca atgtctccca
    26401 cgtatctcct tgtgtattcg ttagaagaag gacttctaaa tgcgcaccag tatctggttt
    26461 ttctccatat aaacgagcag gcagaactcg tgtattattc ataactagcg catcaccagg
    26521 ctgtaactct tcaatgattt catggaaatg tttatcttcc atctctccag ttttccgatc
    26581 caaaacaagt aaacgagaat gatcgcggtc ttttaatggt gtttgtgcga ttaattcttc
    26641 cggcaagtca aaatcaaaat cttctgttgt taacatatcg tcactcactt tctatcatgt
    26701 gcttcatcat tctatcatgt ttttcgttct gttggcaaaa tcagcacttt ttcacttcta
    26761 ttttttgttt tcctaaataa aaaaactacc ctatttgtat tttttcattg ttttaggcat
    26821 actagtaata gatagttctt cattgaaaat agaaaaagag gtggcaccat gattgaattt
    26881 caaaatgtgt caaaaattta taaaggtgga aaagtcgctg tagaggacgt caatctttca
    26941 tttgaaaagg gtgagttcat ttgttttatc ggtacgagcg gaagcggaaa aacgacgacg
    27001 atgcggatga tcaaccgaat gactgatcca acacaaggga aaattttgat tgatggaaaa
    27061 tccatccaag aaattgatcc agtggaattg cgacgtcaga tcggctatgt tatccaaagt
    27121 attggattga tgcctcacat gacgatccgt gaaaatatca ctcttgtcca aaaattgttg
    27181 aaagtcagtc aagaagaacg gaataaaaca gctgaaaaaa tgattgatct tgtggaattg
    27241 ccccgtgaga tgttagatcg ctacccacat gaattatctg gtggtcagca gcagcgtatt
    27301 ggagtcgttc gtgcccttgc agcgaatcaa gatatcatct tgatggacga gccattcggt
    27361 gcgcttgatc caatcactcg tgattccttg caagaccttg tcaaagattt acaagagcgt
    27421 ttgggaaaaa cgatcgtatt tgtaacccat gacatggatg aagcgattaa actagcaagc
    27481 cgtatcgcca tcatgagtga aggccgtgtg atccaatttg atacaccaga aaatatttta
    27541 cgtcatcctg caaacgattt cgtagaagag ttgattggag aagatcgcct actacaagcc
    27601 aagccaaatg cgacaagagt cggcgaagtt atgctaaata cagcaatcac gattacaccg
    27661 gaaaaatcat tacaagaagc aatcaaactg atgagggaaa aacgtgtaga tacgttgtta
    27721 gttaccgata attcgaatgt attaaaagga tttatcgatg tagaaacgtt gaataaaaaa
    27781 cgaggaaaag tatccagtgt cggcgacatt ttgaataaac atgtttttta cgtaaaagaa
    27841 tccgcctatc ttcgtgatac cttgcaacgc attttaaaac gcgggctgaa atacgtgccg
    27901 gtcgttgatg aacagaacaa agtcgtaggt atcctgactc gtgcatcatt agtggatatc
    27961 gtctacgatg tgatttgggg agaagatccc gcaactactg atgttgcaga gtcagctact
    28021 cctgaagaat ctaaggaggt ctgacctatg cagcagtttt ttcatcagca cggttcagaa
    28081 attctctcaa aaggttttga acatctttat atttcaggac ttgctttgat cattggtatc
    28141 atcattgctg taccgcttgg tgtcttactg acacgttttc caaaagctgc cacgattgtg
    28201 attggcttaa ccagtgcctt gcagacaatc ccttctcttg ctttattggc attgatgatc
    28261 ccgctatttg gcgtaggtaa attacctgcg attatcgcac tctttattta ttcattattg
    28321 ccgattttac gtaatacgta cattgggatg aaaaatgtcg attggaatta tcgggatgtg
    28381 gcaaaaggca tggggatgac tgagttccaa tccatttttt ccgttgaatt gcccattgct
    28441 atgccaacga tcatggctgg tattcgttta gctgccgttt acgtgattgc atgggctaca
    28501 cttgcctctt atatcggcgc tggtggttta ggcgatttga tttttagtgg actgaataat
    28561 taccaaccag atctcatttt ggcaggtacg atccctgtaa caattttagc actattggct
    28621 gattggcttc taggtctctt ggaacaccgg ctgacaccga tcgcacttag agaggagaat
    28681 gaagaatgaa acgtatacta aaaatgacca tggttctttt ctctcttctt atacttgcga
    28741 gttgttcctt ccctggtctt gccagcaata cagatgaaga caccatttcg atcacaggtg
    28801 gaatcacttc agaagcacag atcttagcaa gtctagttgc cggaatggtt gaacattata
    28861 cagataaaaa taccgcaatc atcaacaatc tggctactac aactatcaac catcaagcga
    28921 tgatgaacgg agatgcttca atttcagcgg ctcgctacac aggaacagat ttaacgacta
    28981 cattgaacct tcctcctgaa aaagatccga aaaaagcgtt tgctaccgtg aaagatgaat
    29041 ttgagaaaag atacggtcaa acgtggttcc cgtcctatag ttttgaaaat acgtatgtct
    29101 ttcttgttcg aaaagacacc gcacaaaaat accatctatc taaagtcagc gatctgaaaa
    29161 atgtagcgga tgaattagta gctggtgtag atacttcttg gatcaatcgt aaaggagacg
    29221 gttatgacgg cttccaagaa acttatggct tctccttcaa ttcgatatta cctatgcaga
    29281 ttggacttgt ttatgacgca gtcgaagcag gaaaaatgga tattgttctt ggctactcta
    29341 cggatggacg gatcgccagt tatgatctag tcatgttgga agatgatcgt caattcttcc
    29401 caccatatga tgctgctcca gttgtagaca acaagatatt agctgatacg cctcatttaa
    29461 aagaagcctt aaataagtta ggtcatacca tcagtacgga aaaaatgcag gaattgaact
    29521 acgaagcaga taataatcta gtggaaccat cagtagtagc acaacgtttc ttacaagaaa
    29581 accactattt tgaagggaag tgaagcaatt ggataaaatg aatgtctttc aacaattttt
    29641 ctattatttc caggaaaatg gctcctatat ctttgcacaa tttatccgtc acttcctgat
    29701 ttctatctat ggtgtcctct ttgcagcagt tgtcgggatt ccggttggga tcatgatttc
    29761 taggagacgt aaattagcaa actgggtgat tcgtttggcg aatatcatcc agacgatccc
    29821 ttctctagcc atgatctcca ttttaatcat tggattaggc ctgggagtca atgtcgtgat
    29881 tgttactgta tttttgtatt ccctcttacc gatcatcaag aatacgtata ccgggatgat
    29941 ccaagtagac aaaaacatcc ttgatgtcgg caaaggcatg gggatgactg ctagacaacg
    30001 actattcatg gtagagttac cactctctgt ttctgttatc atggcaggca taagaaacgc
    30061 gttagttgtt gccatcggga taaccgcgat cggtgcattc gtcggtgctg gtggtcttgg
    30121 agatatcatt atccgaggaa ccaatgcaac agagggcaca tcgattattt tagcaggtgc
    30181 attgccaact gcattaatgg cgatcatcac ggactggctt ttaggtattt tggaaagacg
    30241 gctagatcca gccagccgta catcacgctg atgttattgt aaaaaacaaa agcttctgcg
    30301 accatttgtt ggtcgcaaag gcttttttag ttctgtcatt tttaaggcaa taatccactc
    30361 tgattcttcc ctccttatgg gtaaagagtt gcttttctta gctatttatc cattccgtat
    30421 actccaactt catactgcca acgggttcat atctgcatcc accgactaag tttttttctt
    30481 atccgctaag gcattttgcc aatccatttt cccatcactc gtcatggaat tcataccaca
    30541 tgatctctaa atactcgtct gcttgttctt tgaattgggc atcaattctt acatcctttg
    30601 gcgtggcttt catttaagcc ttttccatca gtcctcgatt gttcgatgcc ttcagctgca
    30661 tcatcaaaac tggtgactgc cgtatcggta acttcgatca tcttttatac aatcgggaac
    30721 ttgccatcct catccttggc taaaaacata tctagccatt ccagcttctc gccatgggag
    30781 gtttgcggtt cctaaaaccg gtacacgatt cttatggact gcatccgtcg caactagact
    30841 tggcgatacg atgatccttc accagaagag cacccccgta aaccaattga tcgatatatt
    30901 gccaataaga aaagacattg gcgtaaaaga tatcgatccc tcgtgatgca ttgccgttga
    30961 tactgctatt catggccgaa agcgccacga ctttaacttc ttccgtttga gtgacattca
    31021 tgttcggcaa tgtcgctttg gatacccgct ctgctaaagg aacgacactg gtattgtatt
    31081 tcgtatctgt atcttttcca aaagaccatg ccaacgaatc ctctgggaac caataagacg
    31141 actccggttg attgtccatt gcttttttca acatactttt ggctgtttct cgataaacga
    31201 catttccaat tgtcttcgcc tcactttggc gataatcgat ggtcgttgcc actgccaaca
    31261 ctaacaacga tgctccaatt ttactgattt tccttgtcac acttcttcct ccttgtttta
    31321 cataaacccg tcaacattcc attcctaaac tcaaacaatg acccttcaaa ggtggagata
    31381 taggaacaaa ttaaagaaat catgcaataa atccaagaag gttggtttta gaacaaaaaa
    31441 agagtctcct ttttggggag actctttgtt aactatctaa aaaggttggc tcttctcact
    31501 tcactcataa tgtgtccagg cagaataaat ttctacaact ttattttcct catcaacttt
    31561 gtaaaccact cgatgctgtc tattcagcct gcgtgagtat ctgccttcat gtgtaggtct
    31621 cagcttttca aaagactgcg tgggcatata tggatcttcc ttcaatgtct gaatcacttc
    31681 ttcaaattgt ttctttaaat tcgtttgctt gatttttctt aaatccactt tcgcagagtt
    31741 tttgattgcg actgtatagt tactcatcaa agagcttccc aatcaatatc atcgatattc
    31801 gtaaagctac tgtcgtcttt ttcgcggtct cgtactttat ctaaagtacc tgtttgctca
    31861 agaaaaaggg tttcttgaat cgctctccag tcttccagcc caatcaatac agcattgttt
    31921 ccactacgat cactaataat ctcgatttca gtatgatttt catttacctc tttcaataac
    31981 tgataaaaat ttttacgagc agtgctagga ttcatcgttg tattagccat tgtctctcct
    32041 cctttttctg atataattat agcgtacttg attaagtacg tcaaaaaaat acaataaagc
    32101 acttaaattc agcaaacttt tcctcttttt tacatagaga aaaagacccc tttttaggga
    32161 ggatctttgt ccacgctcac ttcttactcc tttacgacta gcggtagcca aacgtaaagt
    32221 aattggtaac aacgtatcat ctttagcaaa ggtcgaatcc aaagcctgtg agtctatgca
    32281 acaacaccaa tacacgaagg ttcctcgttg cagtgtgact tgattgatat cttgaataac
    32341 ctgaggatac gcacgaaaga tttgtaacgg ttattcaaaa ctgattttgg ccgtcaagtc
    32401 ttctactgcc tcaaaaatga ttgcctcttt cgtctcctgt cattcatgtt cactatcgat
    32461 ttgatacttg cttagaccgt tgggtttgtg aatcaccaat taaaaatggc actgagttat
    32521 ggtaacaaaa aatggtcact tgacgaggct gagattccta cttatgaaga cacatggcac
    32581 ttaagagtcg aggtaaagga agaagctgaa aatataggag gctgtacatt atttggcaaa
    32641 ggattcggaa attatgatca agacatcaaa atcaaaatgt tttacagcta aataggaaga
    32701 ataataacat ttcatacgct gttcccatct ttaatggctt atagagggca cctttagctg
    32761 agaataagac aaaacagacc ctctagcttc aattaagagc tagagggtct gtttttgatt
    32821 ttaaaacttg gtttatttct tcaaatggta gctataggtg ctgacaacga tattttcacg
    32881 ccaagacaat cgcaatctta accgcaaact tgtttggtta tggagaatat tctgccagcg
    32941 tctgtttggt acaaattgag gaatcaacac agtagttgta tagttttgtt tcgctgcatt
    33001 tttacttacg atatctacat aacggataat tggattttca atcgagcgat aagaagagtg
    33061 gacgattgaa aaacggacat ctgggaaatg ctttttaaat tctgcttcga tttctttttc
    33121 tttttcgaca tcttcgtcta gcgaaacatg catcgcaacg acataatcac cgatcgaacg
    33181 tgcgtaattc aacgcaccga tatttacccg tgtcacattc ccgactaata caagaacggt
    33241 attcccgtca taatcatgta gttttgcatc attttctaaa cgaagctgtt ctgctacttt
    33301 tttataatga tcatggatct tataaaaaat gaacataacg attggcataa tgatgaagaa
    33361 aggccaaatg tcccctaacc gatagacaaa caaaatcgca atgattgcat atgagatgaa
    33421 cgcacccgta atattggcaa tcgatttgct taaccatctt tttccttctt ttttccattt
    33481 gacgaccatc cctgtttgag ataaagcaaa tggaatgaac acgccgatag agtataacgg
    33541 aatcaagcgt tctgttgagc catggaaaat aaacagcaat acgatcgaac ctagcgctag
    33601 tgtgataatc ccattggaat agcctagtcg atccccacga tcttgataca tgtgaggcat
    33661 gaatttatct tttgccagat tgtatgcaag aactggaaaa gcagaaaaac cggtgttagc
    33721 agcaactgct aaaatcagcg ctgtagcaaa ttgaaggacg taatacaaaa taccatgtcc
    33781 aaagacagct ttaccaattt gagacaaaac agtgacttct ttttcaggaa cgatcccata
    33841 ccaatagttg ataaatgtga tccccacgaa gaagaaacct aagatcaatg ccatcatcgt
    33901 caatgtagca gcggcatttt ttgctcttgg cttcttaaag aatggtacag cattactaat
    33961 cgcctccacc ccagttaaag aggaagaacc agaagaaaat gctcgtaaaa tcaaagcgat
    34021 agaaacaccc ggcacagccg ctcctggaag tgcagtagct tgcaatggca cagcacctgt
    34081 cacgatatta tataaaccaa caacgatcag taacgagatc acaaggataa atgtgtacac
    34141 cggaaataat aggaacgatg cagattccct cagcccacga agattcagca tcatcagtag
    34201 caaaacgatt gtcacagaaa tagcgacttg atgaccgtag agtgctggca ccgctgatgt
    34261 gatcgcttca gcacctgctg agacagatac tgctactgtc aacatgtagt cgatcaataa
    34321 agagccaccg gaaatcaagc cagcattctt tccaagattc tcactgctga cgacatatgc
    34381 tcccccgcca tgaggataag catggatgat ttgacgatat gatagcgtta atgaaatcaa
    34441 taaaataata acaaaagcgg caatgggtag cgaataccag atggctgcag cagacaaagc
    34501 gactaaaacg acaacgattt gttccgtacc atatgcaata gatgataacg catcagacga
    34561 caataatgcc aaagcagcaa atcgggttaa tttgtgctca tcattttcag cagattttaa
    34621 aggcttacct actaacaacc tttttaagta atccaatttc taaactcctt tacacacaat
    34681 tttacttcaa gttttatagt cgaaaattta tagtaaacaa gatgttatag tacgctcact
    34741 gaccttaaaa gtcaataaac tttaagaaaa taattggaaa aaaacagaaa aatatcaagc
    34801 gctttttcaa acgctttaat acagcaaaaa tctaacattt ttttatgggc tttttctttg
    34861 aattctataa taaaaggata gaacttgact tagaaaatca agttctatcc tttttgatat
    34921 cttaactttt ggaagagaga tgtaaggctg ctttctaccc atcattctta ctactcgaag
    34981 catagcgcag ttgtcacaac ctcttgataa acggtgttta aaagcttgca tctgcggtaa
    35041 taagcccaac caaattaaaa acataagaag ctgtttttat ttggtcgttc ttattacttg
    35101 atgcagagca gcttttttac aacctcttgg tacacggtgt tcaatcagct gaccctcggt
    35161 attagctcaa aaaaacggca aaatatgagg aactattttt ccattttttt ctctaatact
    35221 caggtcaaaa cactgatttc acaacctctt tttttaatga aaaatatggc tctcatgcgc
    35281 cgcttctttg aatttcgtca ttttttcttc gatgaacgga tagattgccg ttcctacgat
    35341 tccaaagaca acaaacaaac cgatcatgat ccagattgct ttcgtagcat ttggccaata
    35401 aattcctcct gcagattccc gtaacaagtt taccgcatga gtaaatggca agaacggatt
    35461 gatcatttgg aagaatttcc ctgagacttg gatcggatag ttcccgcctc cgccagagat
    35521 cgacagtact aagatgataa tagcgatccc tttaccgaca ttatcgaata atgcagccaa
    35581 gacatagacg atcatcatga acgctaaggc gatcaagaca gcaaatgaca cgctgtagac
    35641 cggattttgg acatctactc ctagtaagaa aatattccct aatgtaacga tcaagctttg
    35701 cgcaatcgcc attgtcaaaa atgtcagcat acgagcaacg aattgttctc gtttcgtaaa
    35761 attgtttcgg tctttctttc ctaaatagta ttctgtcgta gcaacacttg aaagaagaac
    35821 agcgccgacc cacagacata gtgcagtata aaacggggtg ctagctgaac cgttatttgc
    35881 gattggataa agcgcattgg tttgtagctc aacaggttgt gtaaagaagt cactttcttt
    35941 tgttgcgtct agtttcaata atttgatgac ttcgcctaga tcgacttctt gttcgccttt
    36001 tcggatcgcg tctgctgctt tttggatacc agctttgata tttggccagt cattgttgat
    36061 caagtccgca gcagcgttca cagctgtttc gacttctggc attttttcat ttactgttgt
    36121 caatgtaccg gtgatttcat tttttagtgc tggccaatca ttttggataa aatcagcggc
    36181 caatcctaat ttttgctgaa tgatcggtaa ttcattgttg tataggtctg ccccttcatt
    36241 gatgccattg acgatggtat ccatatttcc attcagcatt gtattggcat catggatttc
    36301 ttgtccaatt gcaggcattt ctttttgata tttttctaaa attgccactg cattcgttac
    36361 agttgctttc gttgagctga gaagcgaatc aaagtcgatc tggtccgctt tgctcagtgc
    36421 gccttgtgca gtcccaatcg tatcgatgac ttggtctagt agagaagtga cagtggaact
    36481 gatttgacct gtattgatac cagcaatgat gccattgata ttattaacaa cggcatcaat
    36541 ttgatctagc aattcatcaa tagccttcgt gtcttcattt tgaacagctg tgttcaaagc
    36601 agataggcgg ttttgcagat tgcttagcaa gggttttaat tgatttagct gatcaataat
    36661 tggctgtaat ttcgtatttc cagcacttgt ttgcaattta gtcaggaact cgaccagttt
    36721 atcgattgca ttttgttgtt gagatattcc gtcaataaag ccgttcaata cattgctgat
    36781 gtttttcttt tcatcatcag tcagcttacc atcttcaata gctgttctta cagtatctat
    36841 taacgaacct gtcgttgttg ccacttgtcc aattgacttc aatgtaacat ctacactgtt
    36901 tgtgatgctt ggtatcgcat ctttcagctg agaggcacct gattttgttt ggtttgctag
    36961 atcacttgcc tgattttcta aatttttcac atctggcaag atatcttgta cttgttggat
    37021 gatcgtcaag ccttgttttg cttcatcgat tccttggttc attgtctctt ggatcgaagc
    37081 aaaatcgccg tcgatctctg ctagttgctt accggcattt tggatctctg ggattttttc
    37141 ttgcaaatca aggatgattt ttgcttgatc tttcaactca ggcattttgt cattcaatgc
    37201 aacgactttt tcacccatct catctacttt agggatgtaa tccacgaatt catttgcttt
    37261 attcagtttt tccttgattt caggtagttg actttgtaat tcgaggactt gttttgtgta
    37321 gccgtcgatc gtgtccaaat tttcatctgt tgataaaatc atatctttaa ctttattgat
    37381 acttactagg ttcgtatcga tgtcgtaacc tatctcattg aatactttca ataaagtcga
    37441 actggctgtt ttgataaatt catttgtgat ttgcgattgg atcgaacttg ctcctttatc
    37501 ggtgattttc ggtgcaatcg cattgatttt ttcatttacc gtatattcga tttttggttt
    37561 cttgatatca ccactagtaa aactcagcag atcttctgag aaatcctttg gcaagtaaat
    37621 acctgcgtaa tactttccgg aacggactcc gtcttctaat tgttcttttg aatcaacgaa
    37681 ttgccatcct aattgtttat ttttatgaag cgattcgatg acttgctccc caatggccac
    37741 ttctttacct tgaaactctg ctggcttgtc tgcactgtaa acagcaattg gcagctctcc
    37801 agtatttcca tatggatccc ataatgcttt gatgttaaac cacgcatata gcgaagggat
    37861 gatcatgatc gcgatgatca gaagtgttgc aatcgggttt ttgaaaatcc gtttccagtc
    37921 aagcaaatat aatcgaagcg tatttttgat tgactgcata gttctccccc ttcttctttt
    37981 ttcagctgaa ttactttacc atgtatttca tacttttttc aatcggcttt tttcaaaaaa
    38041 gtcgaatttt tggacatatc acggcaattg tctcattgta atctcttaca tattgagaaa
    38101 taattaagga ttacagcctt tactttatca tgaaataaaa gggaaagata attttatgcc
    38161 aaagttttta tctattatac gtatacgcaa aaaagctaaa acattttttg tctttgcccc
    38221 tacaaaaatg tttcagcttt tttcgttttt tattcctgta tttcttattt ttcgaatgac
    38281 aggatgattt tcccagcagg aggatgtcct tcaactagct gatgtgcttg gatgacatgc
    38341 ttaagtgaag cagacagatt cattgaaata aaaacatgaa aggctccttt ttggtaggct
    38401 ttgattatgc cagcaaaggc ttctgcatct agatattcct ttcttggaac aaacgattca
    38461 taaaagcctt cctttttttg ccgcaaatct agatctggga ggtcatttaa tgcaacatac
    38521 ctgccgcctg gtttcatgat ctggatgccg gtttctcctt tgatacttcc tttcgtcgca
    38581 tcgattacaa ggtctgcctg atcagcaaat tgcagacctg gatttgtttt atcataagca
    38641 gcaaatgctg aagctcctaa tttttttacc ttttcctcat ttttactgga tgcactggtt
    38701 aagattcgga ttcccttttc atggagaagc tggatcagac ttgacccgac cgcacctgat
    38761 gctcctagta tcattacagt atctgtcggc tggatttccg tcaaatggtt gattagatta
    38821 taagccgtaa ttcctggcgt gaccattccg gctgcttctt cccaactcat tttatctgga
    38881 atttttgcta cttttgctga tggcaacacg acttcttctc cgtaagtccc gccgactgca
    38941 tggacagcta ctcgatcacc tacatgaaca gttgtgacat ctgaaccaat ctctgtcaca
    39001 ataccagcgc catcattacc tggtacataa gggaatttca atgttcgtat ctctttcatt
    39061 tctcctaaac gcaaggcaac atcataagga ttcacactga atgcttttat ttctacacga
    39121 acatgtccag aaccgacttc tctgggatga gcatcgattt cttcaaaaac atcttgtgct
    39181 tctccgtaac cgttaattgc aaatcttcgc atggtcacca tctccctttt tctttaagtt
    39241 tacgtttatg cctttatttt gacaaaaaaa atgtcttttc gttttggcgc atgattcaaa
    39301 acaaaatagt tgtctttttt cgcgtaacgt gttaaattat ttatctaaaa gcgtaaagat
    39361 tacgatttac gctaataaaa aataattgga ggaatttgtt gatggctaaa aaatcaaaga
    39421 ttgccaaagc aaaaaaacag caagaaacga ttgaacgcta tgctgcttta cgccaggaat
    39481 taaaagcgca aaaagattac gaaggcttgc gtaaacttcc tttagatgct cgtccagaac
    39541 gattgaaaaa tcgtgattta atcgatggac gccctcgcgg atacatgcgt aaattcggga
    39601 tgtcccgtat aaaattccgt gaattagcac ataaaggttt gattccagga gtaaaaaaag
    39661 ccagctggta atccaagtct ggctgtaatg taaaggagga gggcgcatgg accactcctt
    39721 ggatcatcaa aaaagatacg ttcgcttctt ctagcagaaa tgctggtact agaagcagac
    39781 gtatcttttt ttacgcacag ctggtccttt tttatatgta aaggctgcat ggttacttcg
    39841 ctatatttat ttagtaacag gagtcagtgc ttcgataccc acactaattt tcaatgctcg
    39901 gtcaacttct gccataatgg gtcgatccaa atggcagacc ttttctttca atcttgattt
    39961 atcgatcgtt ctgatctgct ccaacaggat aacggagtct ctttctattc ctgttccagt
    40021 agctttgatc ccaatgtgag tcggaagctt aggtttagcc atttttgccg ttatggccgc
    40081 aacaatgatc gtcgggctaa aatggttacc tagattattt tgaataacca aaactgggcg
    40141 tgttccgcct tgctccgatc ccacgactgg agaaagatca gcaaaataaa tatctcctct
    40201 ttttaccata ggatcctcct tattcttgat attctcttgg tatccgctgt cctaatccgc
    40261 aagccacttc atagtggatc gtacctaatt gatctgctat atcctgcata gtgatttctt
    40321 tgtctgcatt tttgccgata agcgtcactt ttgttccgac aggaaattct tgaggcaatc
    40381 ggatcatcag ttgatccata catacacgcc caatcgtttc acagtagttc ccttctacca
    40441 atagagaaaa accctgcatc tttcgtggcc agccatctgc ataaccgatc ggtatcgttc
    40501 cgatccactc tgcttcagga gtgatatatg tttctccata gccgattcct tcacctgctg
    40561 ggagtttttt gacttgaatc aattcagaaa ccagctcaag tgctggctgc aatggataaa
    40621 cttccggtag tgcgtgccca gagggattaa gtccgtacat tgctactcca taacgaatca
    40681 tattgccgat tgtttcatca tgccataaag ccgttgcact attgctgaca tgtacatatc
    40741 gaggaagttc gggaagtttt ttcagcactt ctataaagcg ttctttttgc agattccaat
    40801 atgtatcatc tgcctgatct gctgtagaaa aatgggtaaa taaaccttcc cattctaata
    40861 cacgactttc tttgatgaat gcagctgcct cttttacttc ttcaggtgta caaaatccga
    40921 tacgacccat cccagtgtct gcttttagat gaatcgaaag aggcgtctct agcttacttt
    40981 tctctaaaac agcagctgct gcttgcaacc attcccgtgt acctgcagtt actgataaat
    41041 catgagcaac agctacagtc agtgcgtcgt aaggaaccat attcaagata aggatgggct
    41101 gagtaaatcc agcttctctc aactcgactc cttcatctaa atttgaaaca caaaagcccg
    41161 aagctccacc tgcaactgct gcctcagctg tttcaatagc tccatgaccg tatccgttcg
    41221 cttttactac tgcaaacaat tcttttcctt gcggaagtct tgcaacttca tttgctacat
    41281 tttcagttat agctttttta tgtatcactg ctttcgttgg gcggtgccaa gcgacaacca
    41341 tcgtttttcc ttctttctag tcttccaaaa taatttgtgc aaaagctgtt tcatttgtat
    41401 gagtaatact gacccataca ttgccttcat gaggtgaacg cgtcacaaca ggctgaccat
    41461 tttcattttt taaaatctcg acatcttgaa aggtaacttt tccgatccca gttccccaag
    41521 ctttagagaa agcctctttg caagcgtaac gcccgcctaa aaattctact tggcgatgaa
    41581 aaggcaagga ctggaatagt ttcatttcat cagaggtcaa gattcgtgca atgaattttg
    41641 gtttttcttc gatcagccga gtgatcctag gaagttcgac tgcatcgatc ccaattcctt
    41701 ttatcatatg cttttctctt ttcaataaat ttagaacact ctcattctac caaattataa
    41761 ccattttgcc acaaataagt ccacaaataa caaaaatgaa agtttctctt aaaaaagaga
    41821 aactttcatt ttatgtttaa tcgttatttg aacggattac aaatccgcgt tttttatcat
    41881 tgtgacgttt accgccatct ttttggctgt tattattttt gctataaccg ccttttgatt
    41941 tgtttccacg ataattaccg ccgtcacgac gattacggtt gtttccgcca ccacgtttgc
    42001 catttttatt gtatcctttt ttctgcattg gcaatgggcg ttctggtgtg atcttcactg
    42061 gcactgcatc agctggatct tttgctgtcg tttttagcaa aagtgcaact aaatcttgtg
    42121 cagaatattc ttctaataat ttatctgctg tttgcaggta tttatctaga ccattttctg
    42181 caagttttgt ttcgatttgt tcaactgcag cacctaattg acctttgaat gcttcttttt
    42241 cggttggtgg acgtaaagtc gtcatccgtt ttttcgtcaa gttttcaatc acatgcaagt
    42301 agtccatttc atttggtgta acgaaagtca cagacatacc gccttttccg gcacggcctg
    42361 tacgtccgat acggtgaacg tagctttctg gatcttgagg gatatcatag ttgtacacat
    42421 gagtgacacc tgaaatatct aatccacgtg cagcaacgtc tgtcgctaca aggatatcta
    42481 aatgaccgct cttaaatgaa cgcaatacgc tcattcgttt ttgttgagac aaatcgccat
    42541 ggattccttc tgcacggtaa ccgcgtgctt ctaatccgcg tgccaattcg tctacacggc
    42601 gttttgtacg tccgaagacg attgttaatt caggtgtttg aacatcaaac aagcgagtca
    42661 tgatatcgaa tttttcgtat tcttttgcgc ggacatagta ttgatcgatc aagtcagctg
    42721 tcatttcttt cgctttgatc ttcacatgat gtggattttt catgaattta acgccaatat
    42781 tcttgatagc tggcggcatc gttgctgaga aaagtaatgt ttgtctttga tcaggaactt
    42841 gtgaaatgat tttttcgata tcttccaaga agcccatatt caacatttca tctgcttcat
    42901 ccaaaactaa tgtttgaacg gttccaagtt tcaatgtgtg gcggttaatg tggtcaagca
    42961 tacgtcctgg tgtacccaca acgatatgtg gacgatcttt caagccacgg atctgacggc
    43021 caatatctgc tccgccgtaa acggcttgta cacgtacttt tttgtcgcgc cctaaacgat
    43081 ataattcttc ttgtgtttgg atagctaatt cacgtgttgg tgcgataact aaaccttgta
    43141 attcgtgacg atctggatcg attttttcta acattggtaa tccaaatgca gctgttttcc
    43201 ctgtacctgt ctgtgcttgt ccaataacgt cttttccagc taatgctagt ggaatcgttt
    43261 ctgcttggat aggcgttgct tcttcaaaac cagcgcgttc gactgatttc aacaattctg
    43321 gtgataattc tagttcttta aatttcaaat gaatcctcct atatggttgt taactgagga
    43381 tgtagctttt ttgctacccc gtcgatttgt tcggtcaaag tgtattgttt ctttttgaca
    43441 gtggccacgc acgtttttat ccattcttag cccctgtggc accaccgaaa aatcactttg
    43501 cgaagtttag gcattagaaa agccaaacgg gtcgcgttca gctttatcat tattatgaca
    43561 tctggcttca acaagttgtt tctatggcaa aaccagtcca atttctcttc atctttcggc
    43621 aataggttat tctaacatag tttttcgttt tctgcaagtt tacagatttt tatttcattt
    43681 gctgaagttg ttctaccact tcaaataagc ccatgctgtt gctgcctttt agcataatac
    43741 tatctgttgc ttcaagattt tgtttgatag catcaatcaa tttattcttt tctttttcaa
    43801 aatagaacac aggtagttgg gggtattttt ggttcaattc ttcttttagt gcatgcattt
    43861 gttccccata aaggaaaatc atggcgaatt tctcaggatc gatatgttca ctcatttgcg
    43921 catgcatatt taaagactct ttccctaatt ccagcatatc tgctaacaca gcgattctgc
    43981 gtccttttgt tggcagattt ttaaaagtat ctaataccag tcccatggct gttggatttg
    44041 cattgtagac atcgctcaaa atagctgcac catttcctgc cgttagccat tcggttctgt
    44101 tttgagtgat agaaacatgg gcgagccctt ggcgtatctc aggtatggac agtccaaaga
    44161 agcgcccgat cgtatacgca atcaaagcat tcgttacatt ataacttcca ggcagcggga
    44221 tcgtaaatgt ttctccttct actacaaaat cggtttgtgc ttttcctttt gctgtcactt
    44281 ccgcatgaac atcgccattt ccaataccga atgttgtgac tgtttgtact aatgttgccg
    44341 ttaaaggtgt cagcaacggt tcatctgcgg gcacaacgag taatccgtct gatacaagtc
    44401 cgtcagtgat ttccattttg gctttagcga ttccttctcg cgacccaaga ttttctatgt
    44461 gtgcttcacc gatcattgtg attgctgcag cttttggttg agccataagt gaaagttccg
    44521 tgatttcatt ggcgtgatcc atgcccattt ccaaaacgat cttttctgta tcttggggca
    44581 tgtgcaaaat cgtgtaagga agaccaatat tattgttgta atttccttgc gtcttatatg
    44641 ttttgtattt ttcagacaag acagacgcgg ttagatcttt cgtggtggtt ttaccattgc
    44701 ttcctgtaat tgcaacgact tctgcgccta ctttttccaa gtaataggtt gccaattgct
    44761 gcatggcttt caaaggatct gctacaggaa taactggaaa attttttgga gcttgttcaa
    44821 caggcaagct ccaaaatgaa gcagatgctc cgtttgtgat cgcttgctgg ataaattcat
    44881 gtccatctcg cgtaccagca agaggaacga ataaacttcc ttcggttatc ttccggctgt
    44941 caaattcgac attggtaatc tttgtcagtg cagagcattc gctgcccacg gcttgtgcaa
    45001 tttcttgtat tgttagattc attgagatct ctcctaaaaa aggttgtgaa aaaagcgttt
    45061 ggcctaaacg gcgggttcgc atgactctca cttccaagta aatttctcta ttcgtattta
    45121 gaagctagaa tgaacgcggt tacgcaatca ctccagcctc ttctttattt ctctttattc
    45181 atttttttca aaaaaagatt gacgctgatg gtatcgattc attcctaact ggatcagttc
    45241 ttcaatcaaa tcaccgtatt tcaagcccat attttcccat aagagtgggt acatactgaa
    45301 ctccgtaaat cctggcatag agtttaattc attcaggaat aattcatttt tatttgtcaa
    45361 aaagaaatcg caccggctca atccgcttcc acctaacatc gtgtaagcta acttcgcgta
    45421 ctcttgcgct ttttgataaa cttcttctgg cacttcggct ggaatctgca tttcgatttt
    45481 attattgata tattttgctt cataatcata gaatgctacg tcttttacga cttcaccagg
    45541 caaagtcgtc cgaacgtctt catttcctaa tacagcaact tcgatttcgc gcgcttcaat
    45601 tccttgttca acgattgctc gagaatcata ctgataggct gttgctaaag cattttgcag
    45661 ctcttctcgg ttttctgctt ttgtaatgcc gacactagaa cccatattcg ccggtttgac
    45721 aaacatcgga taaagcaaag aaccttcaca ttgatcaaat acttttttag gattttcttt
    45781 ccattgattc ttaagtactg gtacataagg aacttgcggc acaccagcag cttgtaaaat
    45841 atacttggtc atgattttat ccatggcaca tgcactggtc aatacgcctg cgccgacata
    45901 aggcatattc aatgtctcta agaagccttg gatcgttcca tcttccccgt ttggcccatg
    45961 taaaactgga aaaacgatgg ctccttcttc tttgatttcg cccggattga tcacttttcc
    46021 tgtaaagcct tcctctgtct gtccacttgg gtcccatgaa agatgcaaga catctttgct
    46081 ggcaggtttt tctgttagta atggaccttt gacccattgt ccttcttttg taataaatac
    46141 gagttgaact tggtaataat tataataaat ggcatttaaa actgaaaatg cggaaagaat
    46201 ggacacttca tgctctgcgc tgcgtccgcc atatagtaaa gtaatcttca aaagattgtc
    46261 ctcctgtatt ctaaatcgtt cagtgattta ctctcatatt ttagcacaaa acaaaaataa
    46321 acgaataact tatttataaa aaaacgagcc agcttctaaa agaacgagcg tcatttattt
    46381 tttgtcagct agattatttg tcccgttttt tataaaaagt tatgataaag aaaaggaggg
    46441 atgatgatgg cgcaaacaaa tccacgcagc cgcttggcag aaatcattcg agtgttcatc
    46501 aattacaatg tagtgccaaa ctttgtacag caaaaaaatc ccgaacaggt aaaaaaagcc
    46561 tttgaagaac taggccccac cttcatcaag ataggacaga tgctttctgt tcgagaagat
    46621 ttgctttctt ctgccttcac tcaaacattc aaaacattac aagatagtgt acctagcgac
    46681 acattttcaa cggtcaaaaa gacgatcgaa acagaacttt ctctttcatt atcagatata
    46741 ttcgatgatt tttcaaaatc gccttttgcc tctgcttcta tggggcaggc tcatcgtgcc
    46801 aagctgaaga acggtgacgc ggtagtcgtt aagatccaac accctaacat cgccgaagag
    46861 atccgacttg atcttcaact atttgaacga gcgatccctt tgatcaaata cattcctgaa
    46921 actagcgtag ttgatctaaa aggggtactc caagaagtaa aacgttcctt gatcaatgaa
    46981 atggacttct taaaagaaag tcaaaatggc gaacaattct atcaaaagaa caatggctgg
    47041 aaagagatcc gcagccccaa gatttatgac gctttttgtt cgaaaaaagt gatcgtgatg
    47101 gaagaaatgt ctggtaaaaa cttgaatcat ttaatgaacg ccgaaaacaa aactgaaact
    47161 tttatcactg gaattcaaaa caagcagctg aaacaagaag ttgcaaagct tttagttgag
    47221 aactttatga aacaggtgtt tgatgatggt tttttccatg ctgatcctca tccagggaat
    47281 ctgctattcc atgtactaac aaaagaagaa cagacacaag ctagccgaaa aactgaaaca
    47341 gtccatgaaa aagagttcgg ttcctttgct tttcgagcct ccacttctgc agaagaccct
    47401 gttgcgccat ataccatcaa ctacatcgat tttggtatga tggggcatct ttctgcagga
    47461 cttaggcaaa aactcacaca agccgtttta gcgctgtata cgaaagatgc ttatcgaatc
    47521 gaaaaagcgg tccttcgact ttgtcagcaa gaaggctcgt ttgatgaaag tcggttccac
    47581 caagaactga caagttttct tgaacagtat tatgattctc ctattgatga aatcaacttg
    47641 caagaagtat tcacacatgt cgtgacgatt tgtcatcaaa ataatctgca gttcgatcgt
    47701 gatatcacgc ttttgctgaa agcatttggg acattagaag gggtgatccg cgtcctcgat
    47761 cctgaagttt ccctcatgga agtcgcttcg ccttttgctc aacattattt tctcactcat
    47821 ctggatgtag aagatacatt gaaacaaagc ggattagact tattggaagg gatgaaggct
    47881 gcgcctaaga tccctcaaca gctccaccat ctattagaaa tgtggacttc gggacaagga
    47941 aaagtcaatt tggaattgaa aaagcaagac aaattattga gcaggatcga aagtatgatc
    48001 aatcggcttg ttttcggtat gattttagct gcattgatcg tagggtcctc cctgcttgtc
    48061 caagcagcac ctgtagaaaa cgcagaggtc gtttccttat tagggatatt tacgtatgct
    48121 attgccgcat tcgtcattat ttttttggca atcgatgcat tgatccaaat gtataagaag
    48181 aggaaaaagt agaaaaaggt tgtgacagaa agaatcagct tcaagaaata agaaggggtt
    48241 ttacataaat tgtttctcaa atttttgtga aaattcggct tatttttcga agaggctact
    48301 tctgaaacac cgagaataaa aaatgtgaaa aagctgttct gcacccagta ataagaacaa
    48361 tcaaaaaggg aacatggaac acatttatcc tgtttccctg ttcccttttt gatgtattcg
    48421 agcataaata aaataccttc gttacctact atcttctgta cttggtaaac cttagtcaat
    48481 tgcctttttt aacgcttgac gaaaagacat atgatctttc tccgtaaagg gttttggacc
    48541 ctttgtcctt atgccagctt ttcttaactt tccactatga taacgttgtt ctagaagcgt
    48601 gccgatattc gcttcgtcaa attcttttcc agagtgatgg aacactcgca cacccttcgg
    48661 caatacaagt gaagctaaac cataatcttg cgtgatcaaa aagtcattct tcttaaccat
    48721 accgacgatt ttataatccg cactatcagc tccgcggtct acgtagacga tttggacatg
    48781 attagcgtat tctttggtcg tataatggtc cacactggtc acgataacca cgtcaagacc
    48841 ataggattct cctaatttta tgacatcttc ttttactgga gaaccatctc catcaatgaa
    48901 tagcctcatt ctttttcgcc ttcttccata aatgataaac agttactaat ccgctcagta
    48961 aaagaccaac attgatccat tttgccaaga taagcagttg tacactttct atcggcactg
    49021 attcatggtt cagcgtgatc aataatagat ggattggctc tcccatttct tttgctaaaa
    49081 caaacaaaag cgtcatcaat aaaaactggt ggatcacact ccctttattt tgagtagtat
    49141 acttgacaac gatccggcga tccattggaa tagctgtatc agtttgataa tattccttat
    49201 ttagccagct gcctgttact acgtgccaga aactaagcag aaagacacct atcgcaaaac
    49261 catttggaat caagaggacc aataaagaag aactcagtcc aagcaattgc atagccaatg
    49321 gagaccatgt taaacgtttc aaaatccatg gtccgactat ttttgctgca atcaagcctg
    49381 ctgcataagg caaatacata aaaatcgaaa gtttttctaa tccgtacagt atgccaacat
    49441 aaaaactgct aaataaaaaa aggaaattcc ctagcattcc atttagaaaa gtaaacagat
    49501 ttagccagct tggcaacttc cattccttct tctggtgtcc taaaacaaac acgaacaaga
    49561 tgaacagcag caagaaacca taaatagcaa gatcaaaaag cctttcatcg aataacaatc
    49621 gggcatttct caaaagaaac aataagccaa agaatatcaa aaataaaaca agctctcgcg
    49681 tagctaccag atttcttttg atgtctttga aatccagttc gtaacgcgga taatgactaa
    49741 ccgtatggta ggccatgacg taaaaaagtg tataaatagt aaagcttgct gcaatctgag
    49801 tcggtgcagg caactcaatc gcttgataca ggagaaatag ccagatagct gcaaataagt
    49861 actttttccc atccatattg ataaaacctt gcgtcttttc gtgaaaattg accgtggtat
    49921 tagcaggtac aagccatgct gcactcagcc ctagcaaaac acttgaaaaa taaaataaag
    49981 gaaaataaaa tacgcctaat attccaagca atgaaccagc accaccaatc agcagagcga
    50041 tcatcagcaa cgtgtaacta tctagaccaa atcgtagact gttgactaaa aatagtccag
    50101 tcatccgaaa ggcataaaac agcgtaaatg gcaaaatcgt cagccaatta tgcgagtctg
    50161 ttagtcccaa aaataaaaga taaggaataa aaataaccgt gttacttaag ataaagggcg
    50221 ttgtttgtgt aaaattacgc ccaaatcgtt tgagtttttc gaacatgaca atggctcctt
    50281 aaacagtctt ataactaatc tgctcttcga ttttagcatt aaaggggaaa taaaccaatt
    50341 ttacagaata tattcttgta aaaaaaaata cagaaagaag cataagcttc tttctgcatt
    50401 tttacggtac ttgattactc aagcagacaa gcccttctcc acaaaagtct tgcccgacac
    50461 ccctgcatcc ggcaggtgat aaagatagaa atcaattatc ctgtgcctcc aaaatcaatt
    50521 aggagctctg taaaccagag tccttctcga cccgtcgcat aaatttactt taacacagtt
    50581 cctatgttta tgcgagagct tttttcattt tataatcgac catttgaaat ccttgaccga
    50641 tatctgaaat aagttcttct gtaatctcaa atccttcatg cttataaacc tcgattgctt
    50701 gatgattccc ctgattcact cgcaaaaaga tctctctttc ctttgtttgc gcagcaagtt
    50761 cttcatacca gcgaaaaact gaagaagtca atccttgacc acgtacttta tttagcaaat
    50821 ataatttact taaataaatt tttccattta tctcttcata agcagtgtaa ccgacagtct
    50881 tttcttcgct ttttaataaa aagtaggaca caccttctgt cagttctttt tgaatctgtt
    50941 tttttgactg ataagttttc agcatgtaat ccacttgttt ttgaccaatg atcggcacaa
    51001 aaacttccgg ccatacttct tgtatgactg ccatcagctc atccagttca ttttctgtta
    51061 cttttatttt ttccatacac ccacgtcctt ttttacatta taaatgacct tgcgcttgat
    51121 ggaataaaaa gagcatgatc atgctataca cacccaccga ccacggtttc aagaaaatca
    51181 caatctgcat aaattcccta aaagataatt tcgtcaaacc agctactaga cagatcaagt
    51241 cgtctggcgc aaagggaagc agcaaggtaa caactagtac ttttttaata tctttcgttt
    51301 ttcttgtcag caattcatca aatttctgat acttttttgg cgataaaata agctggacaa
    51361 aggctacgcc atagtaacgg acaagcaaaa atccgattgc ttccccaatc actaggccaa
    51421 tacaactata taaaagccca aatccattgc caaacatcag catgcccgct actgaggaga
    51481 tccctcccgg caaaatagga atgatcactt ggatcacttg caacagaaca aatatcatca
    51541 ctgcatactt gccaaattgc tggataaacg cttgcaaaga cgtgctcgat cgaaaaatcc
    51601 catgatgata agcataaaga atcaacacaa agaaaaaaaa cagaccaata atgggcatga
    51661 tctgaagaca tttttgtaaa actgatcctt ttttagacat ctatattccc cacatctttt
    51721 ttcatttatt aaaactgcct cctt
//
LOCUS       DACEEZ010000012        47478 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20313_2017
            SAMN12639966-rid9879223.denovo.012, whole genome shotgun sequence.
ACCESSION   DACEEZ010000012 DACEEZ010000000
VERSION     DACEEZ010000012.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639966
            Sequence Read Archive: SRR10023158
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 47478)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 47478)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741683.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741683.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:44:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,016
            CDSs (total)                      :: 2,962
            Genes (coding)                    :: 2,889
            CDSs (with protein)               :: 2,889
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 36 of 73
            Pseudo Genes (incomplete)         :: 29 of 73
            Pseudo Genes (internal stop)      :: 18 of 73
            Pseudo Genes (multiple problems)  :: 8 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..47478
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639966-rid9879223.denovo.012"
                     /isolate="E20313_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(101..1585)
                     /gene="guaB"
                     /locus_tag="IWO89_000757"
     CDS             complement(101..1585)
                     /gene="guaB"
                     /locus_tag="IWO89_000757"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HAQ0455530.1"
                     /translation="MSNWETKFTKKGFTFDDVLLIPAESHVLPNEVDMSVQLAKNIKL
                     NIPIISASMDTVTDSKMAIAMARQGGLGVIHKNMTISQQADEVRKVKRSESGVIIDPF
                     FLTPQHLVADAEELMSKYRISGVPIVETLENRKLVGIITNRDMRFVTDYHMPIADVMT
                     KDNLVTAPVGTSLKDAEKILQKHKIEKLPIVDNEGRLSGLITIKDIEKVIEFPNAAKD
                     EHGRLLVAAAVGVTSDTFERANALLEAGADAIIIDTAHGHSAGVIRKIQEIRSTFADA
                     TLIAGNVATAEATKALYDAGVDVVKVGIGPGSICTTRVVAGVGVPQLTAIYDAASVAR
                     QYGKAIIADGGIKYSGDIVKALAAGGHAVMLGSMLAGTDESPGEFEIFQGRRFKTYRG
                     MGSLGAMEKGSKDRYFQGSVNEANKLVPEGIEGRVAYKGSVADIIFQMIGGLRSGMGY
                     VGAANLQQLRDEAQFIQMSGSGLKESHPHDVQITKEAPNYSVES"
     gene            complement(2090..2785)
                     /locus_tag="IWO89_000758"
     CDS             complement(2090..2785)
                     /locus_tag="IWO89_000758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1129 domain-containing protein"
                     /protein_id="HAQ0455531.1"
                     /translation="MEPEQLRAIVKENNSLETKLTKRNQQYIFDLKKSLEAANLSEEE
                     KVKALHDILPTLVAEQKGGRTARQLFGTVSERTEAIINKPAEVKNSSTPLLMWLDNTL
                     LLLGLLSIMIAITGLFSRGTTPAYGFSTLVLGSAIGGWVFYMMYKYIYQYEYPGADRS
                     KKPGMGKTLLILGGTTLLWVVLFSATTLLPPVINPVLDPVVVIIIGATALAIRYYLKK
                     KYGIIGSLSVPRR"
     gene            complement(2875..3975)
                     /gene="ychF"
                     /locus_tag="IWO89_000759"
     CDS             complement(2875..3975)
                     /gene="ychF"
                     /locus_tag="IWO89_000759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-regulated ATPase YchF"
                     /protein_id="HAQ0455532.1"
                     /translation="MALTAGIVGLPNVGKSTLFNAITKAGAEAANYPFATIDPNVGMV
                     EVPDWRLQRLTELVHPKKTVPTTFEFTDIAGIVKGASKGEGLGNQFLSHIRQVDAICH
                     VVRCFDDDNITHVEGRVDPLEDIDTINLELVLADLESINKRYTRVAKVAKTKDKDAVA
                     ELAVLDKIKPVLEEGKSARTIEFTEEEQKIVKSLFLLTTKPILYVANVSEDDVADADA
                     NHYVQEVRNFAAEENAEVIVVSARAEEEIAELDEEDKAEFLEALGIEESGLDQLIRAA
                     YDLLGLATYFTAGEQEVRAWTFKKGIKAPQAAGIIHSDFERGFIRAETVSFEDLDHYG
                     NMHAAKEAGRVRLEGKDYVVQDGDVMLFRFNV"
     gene            complement(3984..4175)
                     /locus_tag="IWO89_000760"
     CDS             complement(3984..4175)
                     /locus_tag="IWO89_000760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF951 domain-containing protein"
                     /protein_id="HAQ0455533.1"
                     /translation="MYDLHDIVEMKKPHACQTNRWEVIRMGADIKIRCMNCGHIVMMT
                     RRDFEKKLKKVIEKNAEKE"
     gene            complement(4196..5086)
                     /locus_tag="IWO89_000761"
     CDS             complement(4196..5086)
                     /locus_tag="IWO89_000761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HAQ0455534.1"
                     /translation="MKKSKGLGRGIDALFQDLENLETVDVKDDTVVQLPLNELRPNPY
                     QPRKTFEEVSLQELANSIEQSGVFQPIIVRRSAVKGYEIIAGERRFRASKLAEKETIP
                     AIVRDFDEEAMMQVAVLENLQREDLNPLEEAEAYEMLMKNLKLTQAEVASRLGKSRPY
                     IANYLRLLSLPDLVKEMVQDQRLSMGQARTLLGLKNKTLILKLANRAVTENLTVRQLE
                     QLVSELNQNDGKEKKKIPRLAKEKPYYIRESEDRLMDKFGTSVAIQEKNGKGKIEIEY
                     LSQKDLTRILDILNIQFDEE"
     gene            complement(5073..5840)
                     /locus_tag="IWO89_000762"
     CDS             complement(5073..5840)
                     /locus_tag="IWO89_000762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HAQ0455535.1"
                     /translation="MAQIISVANQKGGVGKTTTTVNLGACLASLGKKVLLVDMDAQGN
                     ATSGVGIRKPDVTRDIYDVLVNELPIDEATLITEHENLSIVPATLQLAGAEIELTSMM
                     ARESRLKGSLAEVSSQYDYILIDCPPSLGHLTINSFTASDSILIPVQCEYYALEGLSQ
                     LLNTVRLVQKHFNPELEIEGVLLTMYDARTNLGNEVVEEVRKYFREKVYETIIPRNIR
                     LSEAPSHGKPIIDYDPRSRGAEVYQALAKEVVSREEK"
     gene            complement(5852..6565)
                     /gene="rsmG"
                     /locus_tag="IWO89_000763"
     CDS             complement(5852..6565)
                     /gene="rsmG"
                     /locus_tag="IWO89_000763"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(527)-N(7))-methyltransferase
                     RsmG"
                     /protein_id="HAQ0455536.1"
                     /translation="MIPEEFRQSLAEKGIELTDHQMNQFAIYYRTLVEWNEKMNLTAI
                     TDEKDVYLKHFYDSLTLAFEVDFTQPLSLCDVGSGAGFPSIPLKIAFPELQITIVDSL
                     NKRITFLSELTRLLGIDASLYHDRAETFGQNPHFRASFDLVTARAVARLNVLAELCLP
                     LAKQEGYFYALKAAKSEEELTEAKQAIALLGGKFLKEEAVTLPNVSDQRHIIVIQKKK
                     PTPKKYPRKPGLPNKQPIK"
     gene            complement(6797..8698)
                     /gene="mnmG"
                     /locus_tag="IWO89_000764"
     CDS             complement(6797..8698)
                     /gene="mnmG"
                     /locus_tag="IWO89_000764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis enzyme MnmG"
                     /protein_id="HAQ0455537.1"
                     /translation="MEHYEADAYDVIVVGAGHAGSEAALAAARMGSKTLLLTINLDMV
                     AFMPCNPSVGGPAKGVVVREIDALGGEMGKNIDKTYIQMRMLNTGKGPAVRALRAQAD
                     KHAYSTEMKHTIEKEPNLTLRQGIVEELIVEDGVCHGVVTSTGASYRAKAVVITAGTA
                     LRGEIIIGELKYSSGPNNSQPSIGLANHLKELGFAIERFKTGTPPRVKSSTIDYSVTE
                     EQPGDKEPNHFSFSTPDSAYKLDQESCWLTYTGETTHQIIRDNLHRAPMFTGIVEGVG
                     ARYCPSIEDKIVRFADKPRHQLFLEPEGLNTEEVYVQGLSTSLPEDVQVNMLHSIAGL
                     EKVEMMRTGYAIEYDVVEPHQLRPTLETKVVENLYTAGQTNGTSGYEEAAGQGLIAGI
                     NAALKVQGKEPLVIKRSDGYIGVMIDDLVTKGTNEPYRLLTSRAEYRLLLRHDNADLR
                     LTEMGHRVGLVKEDQYKNYLKKKEAVEQEIKRLMNVRIKPTEEVQDFLATINAAPLKD
                     GVLASDFLRRPEITYAELLRFIPENEELTRKEIEQVEIQIKYEGYIKKAMEKVEKLKR
                     MEAKKIPENIDYAAINGLATEAKQKLQKIQPETIAQASRISGVNPADLSILMVYIEQG
                     KIAKVSEPA"
     gene            complement(8715..10112)
                     /gene="mnmE"
                     /locus_tag="IWO89_000765"
     CDS             complement(8715..10112)
                     /gene="mnmE"
                     /locus_tag="IWO89_000765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis GTPase MnmE"
                     /protein_id="HAQ0455538.1"
                     /translation="MAEITLEFDTIAAISTPPGEGAISIVRLSGDQAVQLADKVYQSG
                     NKRLSEVPSHTIHYGHIVDPKSNQLVDEVMVSVMRAPKTFTREDVVEINCHGGIVVVN
                     QILQLLLREGARLAEPGEFTKRAFLNGRVDLSQAEAVMDLIRAKTDKAMGLALNQLDG
                     NLSALIRSLRQEILETLAQVEVNIDYPEYDDVEELTTKLLLEKAQMIQQRIQALLATS
                     KQGKVLREGLSTAIIGRPNVGKSSLLNHLLREEKAIVTDIAGTTRDVIEEYVNVRGVP
                     LKLIDTAGIRETEDVVERIGVERSRKALAEADLILLVLNQSEPLTAEDEQLLEATSGL
                     KRIILLNKTDLPAQLEQEKLKKLIENEPVFSISVAKNDGLDRLESAISDLFFSGETGE
                     RDATYVSNTRHIALLEKASLSLEEVIAGIDSGMPVDLVQIDMTRCWDYLGEVVGDSVQ
                     DELITQLFSQFCLGK"
     gene            10456..10794
                     /locus_tag="IWO89_000766"
     CDS             10456..10794
                     /locus_tag="IWO89_000766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0455539.1"
                     /translation="MSPFRSAALEEESDPRINDVVLSTGNINQKYIVRDIVFVVECLE
                     ADLFDPSFPKDDLFLTTKRKLKTKVLEYGANAVINCHFEYQQKKRDGKYFLELFAYGT
                     VIQFIQTTIG"
     gene            complement(10962..11720)
                     /locus_tag="IWO89_000767"
     CDS             complement(10962..11720)
                     /locus_tag="IWO89_000767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KH domain-containing protein"
                     /protein_id="HAQ0455540.1"
                     /translation="MPIYEAATIDEAVQLGLADLGLAKDQVEIEILEEGKKGFLGMGR
                     KNARLSIQPSISEKVSETVEQTVEEILEEEPTAEIEVVNETILEEKTEEVSELGDEAA
                     LTELAVYLTEISKQLDAPALVKMERESNNMVVFHLETQKQGILIGKHGKTLNALQYLA
                     QVFIHRLARNKLSVVVNVGNYREKRQEIIQRLADRTAEKVKRTGQPVFLEPMPAFERK
                     QIHAALSKDEHVKTHSEGEEPYRYLVVEPAKKYY"
     gene            complement(11738..12556)
                     /locus_tag="IWO89_000768"
     CDS             complement(11738..12556)
                     /locus_tag="IWO89_000768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HAQ0455541.1"
                     /translation="MKKYKRLLLMAGIVSLVIVLSGCGTGEVSAQSTGIWDRYIVYYF
                     AEAIKALSFGNAGIGIILFTLIIRVILLPLMHFQTKSMRKTQELQPKLKALQQQYSSR
                     DPETQSKLREEQQRLYAENGVNPYAGCLPLLVQMPILMALWQSISRVPELKQGHFLWL
                     NLGNADPTFILPILAAIFTFASTYLSSMSQIESNASMKIMNFVMPVMILVMGVNLASG
                     LSLYWVVSNAFQVVQTLLINNPFKIRKEREEEARRIRERERALKKAMNPKKKRK"
     gene            complement(12553..12909)
                     /gene="rnpA"
                     /locus_tag="IWO89_000769"
     CDS             complement(12553..12909)
                     /gene="rnpA"
                     /locus_tag="IWO89_000769"
                     /EC_number="3.1.26.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HAQ0455542.1"
                     /translation="MRKAYRVKKEREFQAVFHTGDSFANRKFVVYRLEKPGQPHFRVG
                     LSVGKKVGNAVNRNEVKRKIRAAVYSIKEEIDPELDFIVIARPSVKGLTYDEVRSNLV
                     HVLKLAKIIDREEKSK"
     gene            complement(13027..13161)
                     /gene="rpmH"
                     /locus_tag="IWO89_000770"
     CDS             complement(13027..13161)
                     /gene="rpmH"
                     /locus_tag="IWO89_000770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003397591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HAQ0455543.1"
                     /translation="MKRTYQPNKRKRQKVHGFRKRMSTKNGRRVLASRRRKGRKVLSA
                     "
     gene            13684..15018
                     /gene="dnaA"
                     /locus_tag="IWO89_000771"
     CDS             13684..15018
                     /gene="dnaA"
                     /locus_tag="IWO89_000771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HAQ0455544.1"
                     /translation="MVSLDALWNELKATYQKDLSPASYNTWIETANPRTLDQNQLVVE
                     VPSKIHKEYWEKNLATKIVEVGYMLSGNEIIPRFITGEEAEQEEVIEEKNPKVVAPSP
                     LKKAMLNPKYTFDTFVIGKGNQMAHAAALVVAEDPGSIYNPLFFYGGVGLGKTHLMHA
                     IGHQMLQSQPNAKVKYVSSETFANDFINSIQNKTAEEFRQEYRNVDLLLVDDIQFFAE
                     KEATQEEFFHTFNALYNEGKQIVLTSDRLPNEIPKLQERLVSRFAWGLSVDITPPDLE
                     TRTAILRKKAAAERLEIPDDTLSYIAGQIDSNIRELEGALVRVQAFAAMNSEDISTSL
                     AADALKTLKSGKGHPQLSILQIQEEVAKYYHIQLKDLKGKKRVKSIVVPRQIAMYLAR
                     ELTDNSLPKIGAEFGGKDHTTVIHAHEKIQQLMDSSVSMQNEVSEIKNFLLN"
     gene            15216..16346
                     /gene="dnaN"
                     /locus_tag="IWO89_000772"
     CDS             15216..16346
                     /gene="dnaN"
                     /locus_tag="IWO89_000772"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HAQ0455545.1"
                     /translation="MKVTLNRASFMQELQTVQRAISSKTTIPILTGVKITLTQEGLTL
                     TGSNADISIETFLSVENEKANMQIESTGSIVLQARFFSEIIRRLPEETFTLEVLENKQ
                     VAITSGKANFIVNGLDADNYPHLPVVESHNQMKLPVHVLTKLINETVFAVSQHESRPI
                     LTGVHFILSDNSLLAVATDSHRLSQRVIPVEQAADHFDIVIPGKSLIELSRSLTNEEE
                     IVEISIMENQVLFKTETMYFYSRLLEGNYPDTNRLIPSSFNTEVEFSVPSFLAAIERA
                     SLLSHEGRNNIVRLSIRPDAVVLYGNSPEIGKVEESLSYTASSGDPLDISFNPDYMKA
                     ALRAFGDMSIKVKFISAIRPFTLEPTEDGVQFIQLITPVRTN"
     gene            16575..16817
                     /gene="yaaA"
                     /locus_tag="IWO89_000773"
     CDS             16575..16817
                     /gene="yaaA"
                     /locus_tag="IWO89_000773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S4 domain-containing protein YaaA"
                     /protein_id="HAQ0455546.1"
                     /translation="MKQQVILNTEFMTLGQMLKEVTVIGSGGQAKWYLAENTVLVDGE
                     PENRRGRKLYPGMMVEVPEVGTFFMVKKGNDDNEAE"
     gene            16804..17928
                     /gene="recF"
                     /locus_tag="IWO89_000774"
     CDS             16804..17928
                     /gene="recF"
                     /locus_tag="IWO89_000774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication/repair protein RecF"
                     /protein_id="HAQ0455547.1"
                     /translation="MRLNKLYLKNYRNYEELNIEFSKNLVIFLGENAQGKTNLLESIY
                     VLAMTRSHRTSSEQELIGWTDDQAMIQGEITKGSSTLPLEILLSKKGRKTKINHIEQK
                     KLSTYVGQMNVILFAPEDLSLVKGSPQTRRKFLDMELGQINPIYLYDLVQYQAILKQR
                     NQYLKQLNEKKQTDLLYLDILSEQLAAFGSKVLKARAQFVKRLEFWANSLHQQITHQK
                     EQLEIEYLTAVDSLETHTQEQIQEQFLALLNQNKKKDLFRGTTTVGPHRDDLSFFINQ
                     KNVQTYGSQGQQRTTALSVKLAEIDLIKEETGEYPILLLDDVMSELDDNRQLHLLETI
                     EGKVQTFLTTTTLDHVKNKMTVEPEIFYVQQGKLKGEETE"
     gene            17925..19871
                     /gene="gyrB"
                     /locus_tag="IWO89_000775"
     CDS             17925..19871
                     /gene="gyrB"
                     /locus_tag="IWO89_000775"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003129249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit B"
                     /protein_id="HAQ0455548.1"
                     /translation="MTEERSLVERAKEYDASQIQVLEGLEAVRKRPGMYIGSTSSEGL
                     HHLVWEIVDNSIDEVLAGFATKIHVIIEKDNSITVIDDGRGIPVDIQAKTGRPAVETV
                     FTVLHAGGKFGGGGYKVSGGLHGVGSSVVNALSTQLDVKVYKEGNVYYQEYRRGAVVD
                     DLKIIEQTDRHGTTVHFTPDPEIFTETTEFDFSKLATRIRELAFLNRGMRISIEDKRE
                     EEPVINEYHYDGGIKSYVEYLNANKTVIFPEPVYLEGEQQDIAVEVSMQYTDGYHSNI
                     MSFANNIHTYEGGTHESGFKTALTRVINDYARKQKLMKENDDNLTGEDVREGLTAVIS
                     IKHPDPQFEGQTKTKLGNSEVRTVTDRLFSEHFMKFLLENPSVGRQIVEKGLLASRAR
                     LAAKRAREVTRRKGALEISNLPGKLADCSSNDPEKCELFIVEGDSAGGSAKQGRNREF
                     QAILPIRGKILNVEKASMDKILANEEIRSLFTAMGTGFGEDFDVSKARYHKLVIMTDA
                     DVDGAHIRTLLLTLFYRYMRPIVEAGYVYIAQPPLYGVKQGKNITYVQPGKNAEEELK
                     QVVASLPASPKPSVQRYKGLGEMDDHQLWETTMDPSNRMMARVSVDDAIAADQIFEML
                     MGDRVEPRRAFIEENAHYVKNLDI"
     gene            19891..22362
                     /gene="gyrA"
                     /locus_tag="IWO89_000776"
     CDS             19891..22362
                     /gene="gyrA"
                     /locus_tag="IWO89_000776"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase subunit A"
                     /protein_id="HAQ0455549.1"
                     /translation="MSEEIRENIHDVNLTSEMKDSFIDYAMSVIVARALPDVRDGLKP
                     VHRRILYGMNELGVTPDKAHKKSARIVGDVMGKYHPHGDYAIYESMVRMAQPFSYRYM
                     LVDGHGNFGSVDGDGAAAMRYTEARMSKIATEMLRDINKNTVDFQSNYDDTEKEPVVL
                     PARFPNLLVNGTTGIAVGMATNIPPHNLSEVVAAIDLLMDNPDVTTNELMEVLPGPDF
                     PTGGLVMGKSGIRRAYETGKGSITVRAKVEITEMPNGKERIIVTELPYMVNKAKLIER
                     ISELHRDKRIEGITDLRDESSREGMRIVIDIRRDASASVILNNLYKLTSLQNSFGFNM
                     LAIEKGVPKVLSLKQILENYIEHQREVITRRTVFEKEKAEARAHILEGLRIALDHIDE
                     IIAIIRGSKSDDEAKATMIERFDLSDRQSQAILDMRLRRLTGLEREKIENEYQELLKL
                     IEDLTDILARPERVTEIIKNELADLNQRFGDKRRTELLVGEVLSLEDEDLIEEEEIVI
                     TLTNNGYIKRLANNEFRAQRRGGRGVQGMGIHNDDFVKNLVSCSTHDTLLFFTNNGKV
                     YRAKGYEIPEYGRTAKGIPIINLLGIDSSESIQAIIAVTGEAEEGHYLFFTTRQGTVK
                     RTSVTAFANIRSNGLIAIGLKEDDELVNVLLTDGQSNIIIGTHNGYSVTFKEEAVRDM
                     GRTASGVRGIRLREEDYVVGAALMKEDQEVLVITEKGYGKRTKVSEYPVKGRGGKGIK
                     TANITEKNGPLAGLTTVSGDEDIMLITDKGVIIRFNVSTVSQTGRSTLGVRLMKMEAD
                     TKVVTMAAVEPEAAEQTVEEQGSEE"
     gene            22598..22897
                     /locus_tag="IWO89_000777"
     CDS             22598..22897
                     /locus_tag="IWO89_000777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HAQ0455550.1"
                     /translation="MENTKYEIMYIIRPNIDEEAKTALIERFDAILKDNGAEVIESKD
                     WEKRRFAYEMNGYREGIYHIVKVSSPSSANAVNEFDRLAKINDDIIRHMIVKEEA"
     gene            22947..23459
                     /gene="ssb"
                     /locus_tag="IWO89_000778"
     CDS             22947..23459
                     /gene="ssb"
                     /locus_tag="IWO89_000778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016186236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HAQ0455551.1"
                     /translation="MINNVVLVGRLTKDPDLRYTASGTAVATFTLAVNRNFTNQSGNR
                     EADFINCVIWRKSAETLANYARKGTLLGVTGRIQTRSYDNQQGQRVYVTEVVADNFQL
                     LESRSASENRQQSGGFQSSGQSAGGFGGNNNSNQTSQSSNGMPDFDRDTSDPFGSSST
                     IDISDDDLPF"
     gene            23485..23721
                     /gene="rpsR"
                     /locus_tag="IWO89_000779"
     CDS             23485..23721
                     /gene="rpsR"
                     /locus_tag="IWO89_000779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003129253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HAQ0455552.1"
                     /translation="MAQQRRGGRKRRKVDYIAANHIEYIDYKDIELLKRFISERGKIL
                     PRRVTGTGAKNQRKITVAIKRARIMGLLPFVSED"
     gene            23864..25837
                     /locus_tag="IWO89_000780"
     CDS             23864..25837
                     /locus_tag="IWO89_000780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DHH family phosphoesterase"
                     /protein_id="HAQ0455553.1"
                     /translation="MENKNNHLSPKPLFLILFILIEVLVVLILPFRFVSASVILLMNL
                     WGVRYWLKMKRWLILNKREKIQQASTVAETNLAYVSEVMPVGVIAYQPSSQKIEWLNP
                     LALKIKDQSGLTEEELLDTFFTAKEKKKNQVYLADKTFQFDMDTVKGVVYFNDITQNQ
                     LLKLHQTNSQPVIGILSIDNYADAIDKLDDKEISYLNSFVTTLVSDWMNEYHVFFKRI
                     NAERFFFVAQMIDLKKMMDKKFDLLDRFRAEAEKQSSPITLSMGIAYGSRTLDEIGGE
                     AQNNLDVALVRGGDQVVVKEVKEGAKPVYYGGKSAKNVKRTRVRSRAMSTALRRIFDE
                     TGDVFIMGHKFPDMDALGSAFGVSCLARFNQKKAYIVINENEIIPDVERCLEEIHKHP
                     ELEMQLISPERAIELKKEEDLLVMVDYHKPSLSISQGLYEQFDKIVIIDHHRRGEEFP
                     SKPLLTYIESSASSASELVSELIQYQSSKTNRLDRFEATLLLAGMVVDTNSFNVRTTA
                     RTFDVASYLRSCGADASLVQYLLSSDLATYLEISNLIAGSDYVTSDIVVAAGTDEKEY
                     NSVIAAKTADTLLSMININAAFVITKRTDGLIGISARSTGTINVQIIMESLGGGGHFT
                     NAATQLENTTVEETKQKLFAAIKENMNQIYDKE"
     gene            25843..26295
                     /gene="rplI"
                     /locus_tag="IWO89_000781"
     CDS             25843..26295
                     /gene="rplI"
                     /locus_tag="IWO89_000781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HAQ0455554.1"
                     /translation="MKVIFLQDVKGKGKKGDIKEVPAGYAQNYLIKNNLAKEATKSSI
                     SQLAGQKKAQEKHEAEILEEAKKLKEFLEKEDTVVELKAKAGEDSRLFGSIPSKQIAE
                     GLNKQYKIKLDKRKIELENPIRSLGYTNVPVKLHHEVTATIKVHVVQE"
     gene            26569..27936
                     /gene="dnaB"
                     /locus_tag="IWO89_000782"
     CDS             26569..27936
                     /gene="dnaB"
                     /locus_tag="IWO89_000782"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HAQ0455555.1"
                     /translation="MNEVWQDRIPPQNIEAEQAVLGSVFLDAETIIDAMEYIEPKDFY
                     RRGHQIIFETMIELNDRNEAIDVVTVKDRLEQANLLEDAGGLSYLSDLALAVPTAANI
                     VYYAKIVEEKSLLRTLIQTATGIVTKGFEQGEEVQTILDDAERSILEVSEKRNRSGFL
                     SIAEVLNSTIENIDRLAQNNEEITGLPTGYQGLDKMTAGFQPEELIILAARPAVGKTA
                     FALNIAQNVGTKTDRSVAIFSLEMGAESLVNRMLCAEGSIEASHLRTGQLSEEEWSNL
                     IVAMGSLSRANIFIDDTPGIKISEIRAKCRKLAQEKGNLGLILIDYLQLIEGNGKENR
                     QQEVSDISRQLKKLAKELKVPVIALSQLSRGVEQRQDKRPVLSDIRESGSIEQDADIV
                     AFLYRDDYYEREGGDDDDGNEPKNDNVIEVIIEKNRSGARGTVELLFIKEYNKFSSLS
                     PREEF"
     gene            28716..30008
                     /locus_tag="IWO89_000783"
     CDS             28716..30008
                     /locus_tag="IWO89_000783"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002359213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthase"
                     /protein_id="HAQ0455556.1"
                     /translation="MSSVVVVGTQWGDEGKGKITDFLSENAEVIARYQGGDNAGHTIK
                     FNGVTYKLHLIPSGIFYKDKISVIGNGVVVNPKSLVKELAYLHEKGVETSNLRISDRA
                     HVILPYHIKLDQLQEDAKGENKIGTTIKGIGPAYMDKAARVGIRIADLLDKEIFEERL
                     RLNLDDKNRQFVKMFDSEPLDFDDIFEEYYEYGQQIKQYVTDTSVILNDALDAGKRVL
                     FEGAQGVMLDIDQGTYPFVTSSNPVAGGVTIGSGVGPSKINKVVGVCKAYTSRVGDGP
                     FPTELFDETGEQIREVGREYGTTTGRPRRVGWFDSVVMRHSKRVSGITNLSLNSIDVL
                     SGLETVKICTAYELDGELIYHYPASLKELNRCKPIYEELPGWSEDITGCKTLAELPEN
                     ARNYVRRISELVGVRISTFSVGPDRTQTNILESVWAQI"
     gene            complement(30287..30997)
                     /locus_tag="IWO89_000784"
     CDS             complement(30287..30997)
                     /locus_tag="IWO89_000784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4811 domain-containing protein"
                     /protein_id="HAQ0455557.1"
                     /translation="MIIGVLIVSLLAFALTNVFAKKTWQTFLSLIFGLIFVASLSLIV
                     ANLSNHFGMEKVTETKTEKIVSSADSQGADMLLYKALGNGKEKVYLYRTNEKQEKPKA
                     TGTDNETNKVEKTDGDAEKVTKTTYWEYKNDMYKFWFNIADNNHEYDSRVNTFKIPET
                     WVELSTDQAAKLAELVKKQQSTMESEAKAYVQDGMVKAMTENPKMSKAEQEQRTKALA
                     AEFQQQAFAKLVKEAKGE"
     gene            complement(30994..32466)
                     /locus_tag="IWO89_000785"
     CDS             complement(30994..32466)
                     /locus_tag="IWO89_000785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter"
                     /protein_id="HAQ0455558.1"
                     /translation="MSNNQPVDIYGKPYNRTLLVVVLLIGTFCTVLNQTLLTTAFPTL
                     MKAFDISASDVQWLTTGFLLVNGIMIPITAWLINKFSSRNLYLSAMTIFLIGTITCFT
                     APNFSTLLIGRLIQACGVGVSMPLLQNIMLSIFPPEKRGSAMGMSGIVIGLAPALGPT
                     LSGYIIDNYHWRDLFGMVLPIVVLVLVLAFFLMKSVIPLSNPSIDILSAILSTIGFGS
                     LLYGFSSVGDDGWGSLKVIGFLVVGVIFIALFSWRQLHLEHPFLELRVFKSSTFTIAA
                     VLAGVTNMAMVGAEMVVPLYIQNIRGESAFHSGLMLLPGALIMGAMMPVTGAIFDKYG
                     AKRLAITGMFILTAATAPFAFLTKTTPVLYIVILYAIRMFGISMVMMPVTTSGMNALP
                     NNLISHGTAVNNTFRQIASSIGTAILISVLTNVTKANIPSKSVLDNTPLAYKDQAINA
                     TLSGYHAAFIVAIIFGILGFIIAFLLKKKEPVLMKAGDNA"
     gene            32604..33077
                     /locus_tag="IWO89_000786"
     CDS             32604..33077
                     /locus_tag="IWO89_000786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HAQ0455559.1"
                     /translation="MVGASLKRLLENDQLVVGISELSDIVGVSPRQLRYWEQKGFIRS
                     IEADANCPRKYRLPTVIKVELIKKYLDDGFTLSKAAEKAEIRQKKMHHVRKVFSKAIK
                     DIELIDERYTILQIGNFKEENAKMYIIHDNETDELTYKLLPVDEKADYEQLLKEL"
     gene            complement(33230..33781)
                     /locus_tag="IWO89_000787"
     CDS             complement(33230..33781)
                     /locus_tag="IWO89_000787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HAQ0455560.1"
                     /translation="MARKNTLRYILLGLLSKKKMTGYELNQSFKNEIGEFWQAKHSQI
                     YPELAKMEEQGIIQHQVEITGEKLEKKVYELTEEGKEQLAEWIHTPTNELPVNRDEFV
                     LKLYFVKDVNDPALVEIIQQQRDLHEEKRLHLLERKKTIFPTEKEKHENYGHYLILAH
                     AINRETEYANWLTDVLEESNRKK"
     gene            33886..34416
                     /locus_tag="IWO89_000788"
     CDS             33886..34416
                     /locus_tag="IWO89_000788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenolic acid decarboxylase"
                     /protein_id="HAQ0455561.1"
                     /translation="MKEFKELEDFIGTHFIYTYDNGWEYEWYAKNDHTVDYRIHGGMV
                     KGRWVKDQEADIVKLTDGVFKITWTEPTGTDVALDFMPNEKKLHGMIFFPKWVEEHPE
                     ITVTFQNEHIAEMEAAREKYETYPKLLVPEFATITYMGDAGIDNEDVISEEPYDGMPD
                     DIRSGKYFDENYKRVK"
     gene            complement(34899..35342)
                     /locus_tag="IWO89_000789"
     CDS             complement(34899..35342)
                     /locus_tag="IWO89_000789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAQ0455562.1"
                     /translation="MIRTTTKLSAKELESVLSIWLHANLDSHEFIPSTYWENQVESVR
                     KALPDAVIYLAEIDQEIIGFAGVIDQFIAGIFVKREYRQQRIGTQLMKKIKETNKTLT
                     LTVYQKNQSAIHFYLSQGFTIQTQQKDEAVNEEELVMKWNQNADF"
     gene            complement(35358..36518)
                     /locus_tag="IWO89_000790"
     CDS             complement(35358..36518)
                     /locus_tag="IWO89_000790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HAQ0455563.1"
                     /translation="MKQSKQKQSRRLLFRLFLLICIVVGLLIALYPFYVDSLNSFIDQ
                     KRIEQVQKRTAAEIEVQRKKMEEKKQRLTDQGFNPGTDPFNEKNRNESTTSSQLEEWL
                     IGSINIPKIQLNISLYDRLNGRILENGAGVLQGTSFPLGGNSTHSVISAHSGLPNRRL
                     FTELDRLENGDTFILTVLGEKLAYQVKNIQVVLPDDTSVLTISEGEDLVTLLTCTPYM
                     INTHRLLVTGHRIPYNESVKKEEGKGNQERTMRQWFILAGTIIAVVILLVFIGRLIYQ
                     YRLSKKVLDFSFIISDSSGKPVNGSSFILKHKKKTLTRNGVPFSVQSDHHGKVKLDQL
                     PGGTYRIVSADDPKVAVSFGIRKVKQEKIYFFEGRKLVKELQKNGFYFKINE"
     gene            37025..38725
                     /locus_tag="IWO89_000791"
     CDS             37025..38725
                     /locus_tag="IWO89_000791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0455564.1"
                     /translation="MMNLSKVCKGVRQLKKYGPFLAIFFIALAFLMPHLFTHKLILGA
                     DAPFHYNRFYDAAQQIKYGDFHYFPSLYGFQQSGRIVNAVYGPFFAYLQGLIVLLAGS
                     WFHYQILSNFILYLISGFSMLILLRYVNVRNWVSVPVAVLFMSTYSIQYWSINQGFTS
                     WGTCFYPLCMIPIADFVLHKKFPVIKVAVSVGLMTQIHLLSSIMLALMYIPFYLYYFF
                     NQTEKGKALLQLIKSVLLYFVLTANIWAGLAIVYSGNKILPPFVNREMSQKAIDLDGS
                     YWLHYPKAFPILILSGFLLFIFFQQKNTLFQKIVLYTSLVFFFLSTSLIPWTTLIEKN
                     VPFISLIQFPFRFFVPFTLLLLLYLALSLNQWSEQKWFRLLSIFGILIFSAQTIQNLY
                     QHLEKWENETFVSRHTYLFDTPEEARKTFFSKDMSTSLFVFQKTTPDYLPIYKETKDN
                     KYDRYNEEILENETTFIKTHPGGLLTIKWKNTGDKTVHIPVIVYDRTVLQQNGKTLTD
                     YEVTDIGTPIVKQKKGINELTLHYQTPIYFYFILSLTLIGWFTLLCLFIYHRYKLLRA
                     "
     gene            complement(38827..39660)
                     /locus_tag="IWO89_000792"
     CDS             complement(38827..39660)
                     /locus_tag="IWO89_000792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HAQ0455565.1"
                     /translation="MKFMTLNTHSWLEPEPEKKLQELADKILLEDYEVIALQEINQLL
                     ESEEVEPAKLMKFCQVKNQVPIRKDNFAYRLVQLLKEHGKEYFWSWEMSHIGYDKYEE
                     GNALLTKKTLESQVLTVSQSQKKEDYQTRKILIGKTKIDDQDIFVSSCHFSWWTDKKS
                     GFYFEWKNLENYFLETRVPLFFLGDFNNPVDSQGYYTVRESCLLLQDSYVVANEKGKA
                     ATVEKKIDGWEQNTEKLRIDFIFVPEGMQVKKYQRIFDGIDSPIISDHYGVEIELDVN
                     E"
     gene            complement(39964..42129)
                     /locus_tag="IWO89_000793"
     CDS             complement(39964..42129)
                     /locus_tag="IWO89_000793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAQ0455566.1"
                     /translation="MKKIFSFEFWQKFGKALMVVVAVMPAAGLMISIGKSIPLINPDW
                     TPLVTTGGVIENIGWAIIGNLHILFALAIGGSWAKERAGGAFAAGISFILINRITGAI
                     FGVTSDMLANEDAFTHTLFGTKIMVKGFFTSVLEAPALNMGVFVGIIAGFVGAMAYNK
                     YYNYRKLPDALSFFNGKRFVPFVVILWSTIVALILAVVWPNVQAGINNFGLWIAESQE
                     SAPILAPFLFGTLERLLLPFGLHHMLTIPINYTQLGGSYEILSGAQAGTQVFGQDPLW
                     LAWATDLVNLKGAGDMSQYEFVLHNWTPARFKVGQMIGSSGILMGMAFAMYRNVDPDK
                     KARYKSMYFSAALAVFLTGVTEPLEFMFMFAAVPLYVIYSVIQGAAFAMADILPLRVH
                     SFGNIELLTRTPLAIKAGLGGDLINFVLCVIAFGVVTYFLANFLIKKFNFATPGRNGN
                     YDNDSEETVVSNSGTGTADQQVVQIIHLLGGKENIKDVDACMTRLRVSVNDREKVGTE
                     EAWKRAGAMGLIVKDNGVQAVYGPKADVLKSDIEDLLQSGAEIPEPEMVSTGNGQAGS
                     KQYLGIEQELVSAASGEVIPLSEVKDPVFSQKMMGDGFAVIPTSREVVAPIAGKVTSI
                     FPSKHAIGLETKDGIEVLIHMGIDTVQMNQPAFEIAVKEGQEVGTGTKLAEMDLDVIQ
                     NEEKDPTIMIVFTNDKVEEVVIKQLGTVTAGKVIGSIKM"
     gene            42389..44683
                     /locus_tag="IWO89_000794"
     CDS             42389..44683
                     /locus_tag="IWO89_000794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 65 protein"
                     /protein_id="HAQ0455567.1"
                     /translation="MKQIKRLFAIDPWKISTTQLDKKNLRLQESLTSIGNGYMGMRGN
                     FEETYSGDHHQGTYLAGVWYPDKTRVGWWKNGYPEYFGKVINALNFIAIDISINDQLI
                     DLASLEPEDFYWELDMKNGVLSRTFTITTSTNKVRISFERFLSIVKKESAYIYLTIEM
                     LEGSGTVKLVSKLDGNVQNEDSNYDEHFWNEIDRGTIEEIGYLTTKTIPNNFGIEQFT
                     VTAAMRHFVNGKSVIPDYHEDTLALHGCLSAELSQGETWSVEKEIIVLTSRDIPEAQQ
                     AEKAVSELTNQTNNYIQAKEEQTAAWAERWKLADVVIEGDEEAQQGIRFNLFQLFSTY
                     YGEDERLNIGPKGFTGEKYGGATYWDTEAYAVPLYLALAKPEVTKNLLKYRHNQLPQA
                     IHNAQQQGLKGALYPMVTFTGVECHNEWEITFEEIHRNGAIAYAIYNYTNYTGDTEYL
                     KNEGLEVLAEIARFWADRVHFSKRANKYMIHGVTGPNEYENNINNNWYTNTIAAWVLR
                     YTRENYLTYQKEAAITISEEELAKWKDIAENMYFPVDEELGIFVQHDTFLDKDLMPVS
                     DIPTSDLPLNQHWSWDKILRSCFIKQADVLQGIYFFNDTFTKEEKQKNFDFYEPMTVH
                     ESSLSPSIHSILAAELGMESKAVEMYQRTARLDLDNYNNDTEDGLHITSMTGSWLSIV
                     QGFAQMKTAHDQLRFAPFLPKTWKAYSFHINYRGRLLFISVTDEQVTFELLEGFPLEI
                     YVYEQSYSLADRLEITLRKEEANV"
     gene            44676..45341
                     /gene="pgmB"
                     /locus_tag="IWO89_000795"
     CDS             44676..45341
                     /gene="pgmB"
                     /locus_tag="IWO89_000795"
                     /EC_number="5.4.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-phosphoglucomutase"
                     /protein_id="HAQ0455568.1"
                     /translation="MFKGVLFDLDGVITDTAEFHYRAWRKLGQEIGITIDHTFNEQLK
                     GVSREDSLSLLLAYGGKEHSFSKEEFAELAKRKNDYYLEMIQTIEPKDVFPGVVPLLD
                     SLKEEKIKIALASASKNGPFLLEKMGLTPYFDAIADPAEATNGKPAPDIFLLAAKAVG
                     LKAEECIGIEDAQAGIQAILSSGAQPIGVGRAEDLGEEIPLVPDTTFLTIDYLKEKCH
                     DHG"
     gene            45334..46341
                     /locus_tag="IWO89_000796"
     CDS             45334..46341
                     /locus_tag="IWO89_000796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose mutarotase"
                     /protein_id="HAQ0455569.1"
                     /translation="MDNPEISVVPVPETEIKKITLKNDYLEVVLLNYGARLHQIFAPD
                     KEGKSENVLLSYDRFEDVLTDKSFFGATVGPVAGRIRDASWGEHRLEKNCGSHHIHGG
                     TKGWSFQFWEVEVFKTPSSIGVVFYLKDEFSTYPGPITATITYRLTKNELEMTTSASS
                     LVETICNPTNHAYFNLSGNGTRDIYEHQLTVFLDGVLELDQEKLPTGNWTKKEKLPID
                     FRKSPTLQEILACYPDGLDDVFLLHHPRLSKTSLQLFEKHSGRQMTIATSNKSMVLFS
                     TTGFEADFSVNGKQMHSNYGLAIEPQEIPDIVHFPKFGSINLHPGQERISQTIYRFSA
                     Q"
     gene            46363..47382
                     /locus_tag="IWO89_000797"
     CDS             46363..47382
                     /locus_tag="IWO89_000797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family transcriptional regulator"
                     /protein_id="HAQ0455570.1"
                     /translation="MAITVKDVAKKAGVATSTVSRVINDHPSISETTKKKVRKIMDEL
                     GYVPNMTARNLGKRISSAIGVILPPLDSKERIGNPFYLEIMEAINEEARNYDMTTAIA
                     TAKNFELLLENVQRMHLQKQVDGFILVYSDSQDPVIDYLYQNHIPFTLIGQPYHHENE
                     IVYVDNDNQLLGKQATEFLIQNGHKNILFVTNTTHESLYFERYFGYQKAMMMAGLPAL
                     SSVVLEHPEDYLSFEKTLKENEATALVVIDDIFALRTMQLAQMYGYVIPETLSVISFN
                     NSIFSTLTHPYLTSIDIDISELGRVAMQKLRELIHEELSSGVRLVIPHRLIKRETVVP
                     LENPY"
BASE COUNT        14355 a         9525 c         9064 g        14534 t
ORIGIN      
        1 tcttatttcc gaaggagttg cttctacttc cgccgtttat acgtgtttag agcgtgagac
       61 aaaagtgttc tttacttttg tcccacgctc tttaatgttt ttaagattca actgaatagt
      121 ttggtgcttc ttttgtgatt tgtacatcat gagggtgtga ttcttttaat ccactgccgc
      181 tcatttggat aaattgcgct tcatcacgta attgctgtag attggctgcc ccaacataac
      241 ccattcctga acgcaaaccg ccgatcattt ggaaaataat atctgcaaca cttcctttat
      301 aagcaacccg accttcgatt ccttctggta ctaatttatt tgcttcattg acactacctt
      361 ggaagtaacg gtcttttgaa cctttttcca tcgcgcctaa tgagcccata cctcggtatg
      421 ttttgaatcg acgtccttgg aagatttcga attcacctgg tgattcatct gtccctgcca
      481 acatacttcc tagcatcact gcatgtccgc cggcagctaa tgctttgacg atgtcaccag
      541 aatatttgat ccctccgtct gcaataatcg ctttgccata ttgacgagca actgatgctg
      601 catcataaat agctgtgagc tgaggtacac ctacaccggc taccacacgt gtcgtacaaa
      661 tcgatcctgg tccgatacca acttttacga catctacacc tgcatcatat aaagcttttg
      721 tcgcttcagc cgtcgcaacg tttcctgcaa tcaatgttgc atctgcaaaa gttgaacgga
      781 tttcttgaat ttttcggatc acacctgcac tatgtccatg agctgtatcg atgatgattg
      841 catctgcccc tgcttctaat aaagcattcg cacgctcaaa tgtatcgctt gttacaccaa
      901 cggctgctgc tacaagcaat cgaccatgtt catcttttgc tgcatttgga aattcgatca
      961 ctttctcgat gtctttgatc gtgatcaatc cgcttaaacg accttcgtta tctacgattg
     1021 gtaatttttc gattttatgt ttttgcaaaa tcttttctgc atcttttaga gaggtaccta
     1081 ctggggcagt cactaagttg tcttttgtca tcacatcagc aatcggcatg tgataatctg
     1141 ttacaaaacg catgtcgcgg tttgtaatta tccctaccaa ttttctgttt tcaagtgttt
     1201 caacgattgg cacaccactg atacgatatt tgctcatcaa ttcttctgcg tctgctacaa
     1261 gatgctgtgg cgttaagaag aacggatcaa tgattacgcc actttcagaa cgtttgactt
     1321 ttcttacttc atccgcttgc tggctgatcg tcatgttttt atggatcaca cctagtccgc
     1381 cttgtcttgc catagcaatg gccatcttgc tgtcggtaac agtatccata cttgcactga
     1441 tgattggaat attcaactta atatttttag ctaattgtac gctcatatcc acttcgtttg
     1501 gtaacacgtg actttctgct ggtatcaata agacatcatc aaaagtaaat ccttttttcg
     1561 tgaactttgt ttcccagttg gacattttta gtaccactcc tctaatttag tttatctatt
     1621 aaaataattg attttttcat ttaagtcaat gatctacttg gaatcaagtt agctttctca
     1681 tagaatacca atataaagcg tttacatctg tgtatattaa aagagattgt aaaaaagctg
     1741 tcctgcatca agtaataaga acagccaatc aaaaacagct tctcatgttt ttgaattggt
     1801 tgggtttatt accgcagatg caaacttttg aacaccgttt agtggagagg ctgtgaaaaa
     1861 gctgcttagt ttcgagtagt aagaaaaatt ttgtgaaaat ggctttccac atttttgcat
     1921 aaattggact taataccgag aaactggctt ttgaacaccg tttatcgtaa aaagggactg
     1981 tgacaagact tttgtcacag tcccttacta ttatttaact atggataaac aaaatgaggt
     2041 tctttactac ccctagatat ctagcaaaat agctgatctt tttgctagtt tatcttcttg
     2101 ggacggataa acttccaata atcccgtatt ttttcttcaa gtagtaacgg attgctaaag
     2161 cagttgcccc gataatgata actactactg gatctaatac tggattgata actggtggca
     2221 atagtgttgt cgcagaaaac agtacgaccc aaagaagtgt cgttccacct aggataagca
     2281 atgtcttacc cattcctggt ttcttgcttc gatctgctcc gggatattca tattgataga
     2341 tgtatttgta catcatataa aagacccagc caccgattgc agaacctaga acgagtgtag
     2401 aaaagccata agctggcgtt gttccacgtg aaaacaatcc ggtaatcgca atcatgatac
     2461 ttaatagacc aagcaacagc aacgtattat ctaaccacat caataaaggg gtggatgaat
     2521 tttttacttc tgctggctta ttgatgattg cttccgttct ttcagagact gtcccaaaca
     2581 attgacgggc tgttcttccg cctttttgtt ctgcaactaa agtaggcaga atgtcatgaa
     2641 gggcttttac tttttcttct tcagacaaat tcgctgcttc taaagatttt ttcaaatcaa
     2701 aaatgtactg ttgatttctt ttggttaatt ttgtttctaa actattgttt tcttttacga
     2761 ttgctcgtaa ttgttctggt tccattatct aatagtcctc cttagccgtt ccttttctca
     2821 acggtcgtca caaaagacat tctaccatat tctacagcta gtttgatagc ccttttacac
     2881 attaaagcgg aataacatta cgtcgccgtc ttgtacgaca taatcttttc cttctaatcg
     2941 aacacgtcca gcttcttttg ctgcatgcat gtttccgtaa tgatccaagt cttcaaaaga
     3001 gacagtttct gcacggataa atccgcgttc gaaatctgaa tggatgattc ctgctgcttg
     3061 tggtgctttg attccttttt tgaatgtcca agcacgtact tcttgttccc cagcagtaaa
     3121 gtaagtagct aagcctaaca aatcataagc agcacggatc aactgatcca aacctgattc
     3181 ttcgattcct aaagcttcta aaaattccgc tttgtcttct tcatctaatt ctgcgatttc
     3241 ttcttctgca cgagcactga ccacgatcac ttctgcattt tcttctgcag caaagttgcg
     3301 cacttcttgt acataatgat tcgcgtctgc atctgctacg tcatcttctg agacattggc
     3361 tacataaaga attggttttg tcgtcaataa aaatagtgat ttaacgattt tttgttcttc
     3421 ttcagtaaat tcgatcgttc gggcagattt tccttcttcc agtacaggtt tgattttatc
     3481 cagtacagct agttctgcca ctgcatcttt atctttggtt ttagcaactt ttgctacacg
     3541 agtatagcgt ttgttgatag attctaagtc tgctaacact agttccaagt tgatcgtatc
     3601 aatatcttct agtggatcaa cacgtccttc tacgtgagta atattatcat catcaaaaca
     3661 gcgaacaacg tgacaaatag cgtctacttg gcggatatgg cttaagaatt gatttcccaa
     3721 accttctcct ttacttgcgc cttttacgat tccagcaata tctgtaaatt caaaagtcgt
     3781 cggaacagtt tttttcggat gtacaagttc tgttaagcgt tgcaaacgcc agtcaggtac
     3841 ttctaccatc cctacgttgg ggtcgatcgt cgcaaaggga tagtttgctg cttctgctcc
     3901 tgcttttgtg atcgcattaa acaaagttga ttttcctacg ttgggcaacc caacaattcc
     3961 agcagtcaat gccataaatt tattcactct ttctctgcat ttttttcaat tacttttttt
     4021 aatttctttt caaaatctcg acgggtcatc atgacgatat gtccgcaatt catacatctg
     4081 attttgatat ctgcgcccat tcgaatgact tcccagcgat tcgtctggca agcgtgaggt
     4141 tttttcattt caacgatatc gtgcaagtcg tacataaaaa tagcctcctt tttgtttatt
     4201 cttcatcaaa ttgaatattc aagatatcaa gaattcgagt caaatctttt tgagaaagat
     4261 attcaatctc gatcttacct ttcccatttt tttcttggat agctacactt gtaccaaatt
     4321 tatccatcaa acgatcttcg ctttctcgga tataatatgg tttttccttt gctagccttg
     4381 ggattttttt cttttctttt ccatcatttt gatttaactc agaaacaagc tgttccagtt
     4441 ggcggacagt taaattttca gttactgcac gattggctaa tttcaagatc aatgttttat
     4501 tttttaaacc taacagcgta cgggcttgtc ccatagataa acgctgatct tgtaccattt
     4561 ccttgacaag atccggtaaa gaaagcaagc gcaaataatt ggcgatataa ggtctgctct
     4621 ttcctaaacg actagctact tctgcttgtg ttagtttcag gtttttcatc agcatctcat
     4681 atgcttctgc ttcttctaac ggattcagat cttctcgctg caagttttcc agcactgcta
     4741 cttgcatcat cgcttcttcg tcaaagtctc taacgatagc agggatcgtt tctttttcag
     4801 ctagtttgga cgctcgaaaa cgtcgctccc ctgcaataat ctcatatcct tttactgcgg
     4861 aacggcgtac gatgatgggt tgaaagacac ctgattgttc aatagaatta gctagttcct
     4921 gcaaagatac ttcttcaaat gttttacggg gctgatacgg atttggtcgc agctcattca
     4981 atggtagctg gacaacggta tcatctttta catccaccgt ctctaagttt tctaaatctt
     5041 gaaacagtgc atcgattcct cggcctaatc ctttactttt cttcacgact taccacttcc
     5101 tttgccaatg cttggtagac ttcagctcct cgagaacgtg gatcgtagtc gatgatcggt
     5161 tttccatgac taggtgcttc tgataaacga atatttcttg gaatgatcgt ttcgtaaact
     5221 ttttcgcgga agtatttacg tacttcttct acgacttcat ttcccagatt tgttcgtgca
     5281 tcatacattg tcagtaagac tccctcgatc tctaattctg gattaaagtg tttttggact
     5341 aaacgaaccg tattcagtaa ttggcttaat ccttccaatg cgtaatactc acactgaaca
     5401 ggtatcaaaa tcgaatcact tgctgtaaat gaattgattg tcagatggcc aagagaaggc
     5461 gggcaatcga tcaaaatata atcatactga ctgcttactt ctgctaaaga tccttttaat
     5521 cttgattctc ttgccatcat tgacgtcaat tcgatctctg ctcctgctaa ttggagagtc
     5581 gcaggcacaa tactcaaatt ttcgtgttca gtgatcaaag ttgcctcatc aattggcaat
     5641 tcattgacta gaacatcata gatgtctctt gtcacatcag gttttctgat cccgacacca
     5701 ctggtagcat ttccttgtgc atccatatcc actagcaata cttttttgcc aaggctagca
     5761 agacaagcac ctagattcac cgtcgtcgtt gttttgccga cgccaccttt ttggttcgca
     5821 acagatatta tttgtgccat ctcttctcct cctatttgat tggctgctta ttaggcagtc
     5881 cgggctttct tgggtatttc tttggggttg gtttcttttt ttgaataacg atgatatgcc
     5941 gttgatcaga gacattcggc aaggtaaccg cctcttcttt gaggaattta ccacctaata
     6001 aagcaatcgc ttgttttgct tctgtcaatt cctcttcact ttttgctgct ttcaatgcat
     6061 aaaaatagcc ttcttgttta gccaagggca aacacaattc cgctaacaca ttcaaacgag
     6121 caaccgcacg agcagtgaca aggtcaaatg atgcacgaaa gtgaggattt tgcccaaatg
     6181 tttctgcccg gtcatgatat aaggaagcat cgattcctaa taatcgggtt aactcagata
     6241 agaaggttat tcgtttattc agagaatcaa cgatcgttat ctgcagttct ggaaaggcaa
     6301 tttttaaagg tatgctagga aaccctgccc ccgaaccaac atcgcataaa gataaaggtt
     6361 gcgtaaagtc tacttcaaat gctaatgtaa gtgaatcgta aaaatgcttc agatatacgt
     6421 ctttttcatc cgtaatagct gtaaggttca tcttttcgtt ccattcgact aatgtccgat
     6481 aataaatagc gaattgattc atttgatgat cggtaagctc tatccctttt tctgccaagg
     6541 attggcgaaa ttcttctggt atcataaata ttcctttcat tgtgaaacag caattgtttc
     6601 acagattctc tccctacttt aacgcaaaat ctctagacat gcaatccttc catatggatt
     6661 cttaaaaatt tgaaaagtaa gaaaagccga tagacttttt ctttctagta tgactcataa
     6721 aatatacaca gaagagagaa ctagctttga gcaatttctg caaaaactag ttctctcttc
     6781 tatttattat tacgtattac gctggttcac tgactttcgc aatcttccct tgttcgatat
     6841 agaccattag gatacttagg tctgcagggt ttacgccact gattcggcta gcttgggcaa
     6901 ttgtttctgg ttggattttc tgcaattttt gttttgcttc tgttgctaat ccgttgattg
     6961 ctgcgtagtc gatgttttct ggaatctttt tcgcttccat gcgttttaat ttttctactt
     7021 tttccatggc ttttttgatg tagccttcat atttgatttg gatttctact tgttcgattt
     7081 cttttcgtgt caattcttca ttttctggaa taaaccgtag aagttctgca taagtaatct
     7141 caggacgtct taaaaagtcg cttgctagta caccatcttt taatggtgcc gcattaatcg
     7201 ttgctaaaaa gtcttggact tcttctgttg gtttgattcg tacattcatc aaacgtttga
     7261 tttcttgttc aactgcttct tttttcttca aataattctt gtattgatct tcttttacta
     7321 agcctacacg gtgacccatt tctgttaatc gcaaatctgc attatcatga cgtaaaagca
     7381 gacggtattc agcacgtgaa gtcagcaaac gataaggttc gttcgttcct tttgttacca
     7441 aatcatcgat catgacaccg atgtaaccgt cacttcgttt gatcactaaa ggttcttttc
     7501 cctgaacttt caaagctgca ttgataccgg caatcagccc ttgtccagcc gcttcttcat
     7561 aaccgcttgt accatttgtt tgtccagctg tataaagatt ttcaacgact ttagtttcta
     7621 atgtcggacg gagctggtgt ggctctacta cgtcatattc gatagcatat cctgtacgca
     7681 tcatttctac tttttctaat ccagcaatag aatgaagcat gttgacttga acatcctctg
     7741 gtaaagaagt agataaacct tgaacataga cttcttccgt atttaagcct tctggttcca
     7801 aaaataattg atgacgtggt ttgtctgcaa aacgcacgat cttatcttcg atggatggac
     7861 aatagcgagc gcctacccct tcaacgatcc ctgtaaacat tggggcacga tgtaagttgt
     7921 ctcgaataat ttggtgagtc gtctcacctg tataggtcag ccagcaagat tcctgatcta
     7981 gtttataggc actatctggt gtgctgaagc taaaatgatt cggctctttg tcaccaggct
     8041 gctcttctgt aacagaatag tcgatcgtac ttgattttac tcgaggcggt gttcctgttt
     8101 tgaaccgttc gatagcaaag ccaagttctt ttagatgatt cgctagtcca atagaaggtt
     8161 gagagttatt cggacctgat gaatatttca attcaccgat aatgatttct ccgcgaagtg
     8221 ctgttccggc tgtaataaca actgctttgg cccggtagct cgctcctgtg gacgtcacta
     8281 ctccatgaca aacaccatct tcgacgatca gttcttcgac gattccttgt cttagcgtca
     8341 aatttggttc tttttcgatc gtatgtttca tttctgttga ataagcatgt ttgtctgctt
     8401 gtgctcgcaa tgctcggaca gctgggcctt ttcctgtgtt caacatacgc atttggatat
     8461 aggttttatc gatattctta cccatttctc cgcctaacgc atcgatttct cgaacgacaa
     8521 cgcctttagc tggtccgcct acagaaggat tacatggcat gaatgctacc atgtccaaat
     8581 ttattgtcag taataatgtt tttgatccca tacgtgcagc agcaagtgca gcttctgaac
     8641 cggcgtgtcc tgcgccaaca acgatcacgt catacgcatc tgcttcataa tgttccaatc
     8701 gtttgttcct ccttttattt tcctaaacaa aattgactga ataattgagt gattagctca
     8761 tcttgtacac tgtcgccgac aacttctcct agatagtccc agcaacgcgt catatctatt
     8821 tgaacgagat ccacaggcat tcctgaatca atacctgcaa tgacttcttc aagtgagagg
     8881 gaagcttttt ctaacaacgc gatatggcgg gtattagaaa cataagtagc gtctctttct
     8941 cctgtttctc cgctgaagaa taaatcagaa atagctgatt ccaaacgatc tagtccgtcg
     9001 tttttagcta cggaaattga aaaaactggt tcattttcga tcaatttctt caatttttct
     9061 tgttccagct gtgctggcaa atctgtttta tttagaagaa tgatccgttt caatccactt
     9121 gtcgcttcca ataattgttc atcttctgct gtcagtggtt cgctttggtt tagaacaagt
     9181 aagatcaagt ctgcttctgc taatgctttt cggcttcttt cgaccccgat ccgttccacc
     9241 acatcttctg tttcccgaat tccagctgta tcgattagct tgagcggtac cccccgaaca
     9301 ttgacatact cttcgattac atctcgagtc gttcctgcaa tatccgtaac gattgctttt
     9361 tcttctctta acaaatggtt taacaaactt gatttgccga catttggccg gccgatgatt
     9421 gccgtactta acccttcccg cagaaccttc ccttgcttgg atgtagcaag tagcgcttgg
     9481 attctttgtt ggatcatctg cgctttttca agcaataact tcgtagtcaa ttcttcgaca
     9541 tcatcatatt ctggataatc aatatttact tctacttgtg ctaatgtctc taaaatctct
     9601 tgtctcaatg agcggatcaa agcagacaga ttgccgtcta actgattaag agctagtccc
     9661 attgctttgt ctgtttttgc ccgaatcaaa tccatcacag cttctgcctg tgaaaggtca
     9721 acacgaccat tcaagaatgc tcgttttgtg aattcgcctg gctcagctaa tcttgcacct
     9781 tctcttaaca atagctgcaa aatttgattg actacaacga tcccaccgtg acaattgatt
     9841 tctactacat cttcccgcgt aaatgtcttt ggtgctctca tcacggagac catgacttca
     9901 tcgacaagct gattgctctt gggatcaacg atatgtccat aatggatcgt atgacttggt
     9961 acttctgata atcgtttgtt tccagattga taaactttat ctgctagttg cacagcttga
    10021 tcgccactta aacgaacgat actgattgca ccttctccag gtggggtaga aattgccgca
    10081 atcgtatcaa attctaaagt aatttcagcc attttttcac ttcctttttc attttgcgca
    10141 caaaaaaagt gcccactcca ccctttatgc acaataaacg gtgaacttcg cactttgact
    10201 gcttgttctt gattaaattc tggtcataga ttatcacact tgctataagg aatcaagtgt
    10261 ttaaaacaat tagatgaagt tgagcctgtt ctgcttccat taccttatat ttaattgtcc
    10321 ttggatgtct ctttgcgttc tttggtttat tcgaagagaa gatactgtat aaacaaggct
    10381 ttatctttct taaaaaaaac ggtacactaa atggtagcgc tatctaaact atacactttt
    10441 gaaaggagaa aatatgtgag cccgttcaga tctgctgcat tggaagaaga aagtgaccct
    10501 cggatcaacg atgtcgtttt atctacaggg aatatcaacc agaaatatat cgtccgcgat
    10561 atcgtttttg ttgtggaatg cttagaagct gatttgtttg acccatcttt tcccaaagat
    10621 gaccttttct taacaacgaa gaggaaatta aagacgaagg tacttgaata tggtgcgaat
    10681 gcagtaatca actgtcattt tgagtatcag caaaaaaaac gcgacggaaa gtattttctg
    10741 gaactttttg cttatggaac agtgatccaa tttatccaaa caacgattgg ttaaatgaaa
    10801 cagacaaaaa aagcggatca aaactgaaga cagtttcgat ccgctttttt ctatcaaagt
    10861 tagtgactac ttcatcgtat actctgataa gaatgatttc tagtaaaata tacttggttt
    10921 gaaaaacttt gtcaacgtga tttattcaaa aagtaatgga atcaataata ttttttagca
    10981 ggttctacta ctaaataacg ataaggttcc tcaccttccg aatgtgtttt tacatgttca
    11041 tctttgctta atgcagcatg gatttgttta cgttcaaatg caggcattgg ttctaagaaa
    11101 acaggctgcc ctgtccgttt tactttttca gccgtgcgat cagctaaacg ctgaatgatc
    11161 tcttggcgct tttcacggta attgcctaca ttgactacta cagacagctt atttcttgct
    11221 aaacgatgga tgaagacttg tgccagatat tgcaatgcat tcagtgtttt tccatgtttc
    11281 ccaatcagta tgccttgctt ttgtgtttct aaatggaaaa caaccatatt atttgattct
    11341 ctttccattt tgacaagagc tggagcatca agctgttttg aaatttcagt aagataaact
    11401 gccaattccg ttaaagctgc ttcatcacct agctcactta cttcttctgt tttttcttca
    11461 agaatcgttt cgttgactac ctcgatctca gctgttggtt cttcttccaa aatttcttct
    11521 acagtctgtt ctactgtttc agaaactttt tcgctgatac ttggctggat cgaaagccgt
    11581 gcattctttc gacccattcc taggaaacct ttttttcctt cttccagtat ttcgatttcg
    11641 acttggtctt tggccaaacc caaatcagcc agacctagtt ggacagcttc gtctattgtt
    11701 gcagcttcat aaatcggcat tagcattacc tcccctatta tttccgtttt ttcttaggat
    11761 tcattgcttt cttaagagca cgttctcttt cgcggattct ccgtgcttct tcttcacgct
    11821 cttttcgaat tttgaaaggg ttgttgatca gcagcgtttg tacaacttgg aaggcattgg
    11881 aaactaccca gtataatgaa agaccgctgg ctaaattgac acccatcact aaaatcatta
    11941 caggcatcac gaaattcatg atcttcattg atgcattcga ttcaatttga ctcatgctag
    12001 ataaatacgt acttgcaaaa gtaaagattg ctgctaaaat cggcaaaata aatgttggat
    12061 ccgcgttccc taagttcaac cataagaaat gcccttgttt caactctggt acacgtgaaa
    12121 tagattgcca taacgccatc aagattggca tttgtactaa taaaggaaga caaccagcat
    12181 atggattgac gccattttct gcatataaac gctgctgctc ttctcttaac ttactttgtg
    12241 tttccggatc tcttgaacta tattgttgct gcagtgcttt taatttcggt tgtaattctt
    12301 gcgttttacg catgctcttt gtttggaaat gcatcaacgg caataggatc acgcggatga
    12361 tcagtgtaaa taaaatgatc cctatccctg cattaccaaa tgacaacgcc ttgatcgcct
    12421 cagcaaagta ataaacaata taacgatccc aaattcctgt actctgtgcg ctgacttccc
    12481 cagttccgca accggataag acgatgacca aactaacgat accggccatc aaaagtaaac
    12541 gtttgtattt cttcacttac tcttttcctc tctgtcgatt atttttgcaa gttttaagac
    12601 gtggactaaa ttggagcgaa cctcatcata agtcaacccc ttcacacttg gtctagcaat
    12661 cacaataaaa tccaactccg gatcaatttc ttcttttata gaataaactg ctgctcggat
    12721 ttttcgtttg acttcattgc ggtttaccgc gttgccgacc ttttttccta cagataaacc
    12781 aactcgaaaa tgaggctgtc ccggtttttc caaacggtaa acgacgaatt tgcggttagc
    12841 aaaagaatct cccgtatgga acacggcttg aaactctcgt tcttttttta ctcgatacgc
    12901 ttttctcatt agttttcttt ccttttctat ctctcactta cgctcataat cataccataa
    12961 ttacgattgt cctttcatca tttccctaaa aaagagtata aaaaaaacca ctgaaacccc
    13021 agtggcctaa gctgaaagaa cttttcttcc tttgcgacga cggctagcta acacacgacg
    13081 tccattttta gtactcatac gtttacggaa tccgtgaact ttttgacgtt tacgtttatt
    13141 tggttggtat gttcttttca tatattccac ctccaaaatg ttcttatcta ttttataaac
    13201 acagacatac tttgatagta taacgacaaa gtagtgattt tgtcaatata aaatagaaga
    13261 tcgattgtta aaacttatcc acaagattat ttttttctat gggaatccta tgtggaaaaa
    13321 gttatgcaca gggattatcc acagataata aaaggctggt tttacacaga aaacacacgt
    13381 gtaaaaaaag taatccacaa tcaggcgatt tcctgtgtat tccgtctcct aactattggt
    13441 atatctgtat tttttatcca cagacgcaag aggaaatcta ttggttttta cgagttttcc
    13501 acctgtgcat tagtggtgtg gaaaactttt tttgacaact gtgtttttct cttttttctt
    13561 tttgtcgatt gtgtggataa ttgtaattaa aaaatttttt tcttcggttt ctgtggaaaa
    13621 ctattgaaaa aaatgctaaa ttagtactag ttattcactt tttaatgaag gaggaggcct
    13681 tatatggtat ccctcgatgc tttatggaat gaattaaaag caacatacca aaaggattta
    13741 tcgcctgcta gctataatac atggatcgaa acagcgaacc ctcgaaccct tgatcaaaac
    13801 cagctcgttg ttgaggttcc tagcaagatt cataaagaat attgggaaaa aaacctggcg
    13861 acaaaaatcg ttgaagtcgg ttatatgtta tcaggaaatg agattatccc tcgctttatt
    13921 actggcgaag aagcagagca agaagaagtt atagaagaaa aaaatccaaa agttgtggca
    13981 cctagtccgc taaaaaaagc catgctgaac cctaaatata cctttgatac gtttgtcatt
    14041 ggtaaaggaa accagatggc tcatgcagca gcgctcgttg tagcagaaga tccaggttct
    14101 atttataacc cgctcttctt ttatggaggc gtgggacttg gtaagactca cttgatgcat
    14161 gcaattggtc atcaaatgct gcaaagtcag cctaatgcca aagtaaaata tgtaagcagt
    14221 gaaacttttg ctaatgattt catcaattcg atccaaaaca aaacagcaga agagttccgt
    14281 caagaatatc gaaatgttga tttactgcta gtggatgaca ttcaattttt tgcagaaaaa
    14341 gaagctacgc aagaagagtt tttccatact ttcaatgcct tgtacaatga agggaaacaa
    14401 atcgtactaa ccagtgatcg gttgccaaac gagattccga aactgcaaga acgccttgtt
    14461 tctcgctttg catgggggct gtctgttgat attacaccgc cggatcttga aacaagaaca
    14521 gcgattttac gtaaaaaagc ggcggctgaa cgtctagaga tcccagatga cacactaagc
    14581 tacatcgcag gacaaattga ttctaacatt cgtgaattag aaggtgcgct tgttcgagtg
    14641 caagcttttg ctgcgatgaa tagtgaagat atttcgacta gtctagctgc agacgcactg
    14701 aaaacgttaa aatcaggaaa aggacatcct caattgtcaa tcctgcagat ccaagaagaa
    14761 gtggcaaagt attatcacat ccaattgaaa gatctcaaag gaaagaaacg cgtcaaatct
    14821 attgttgttc ctagacaaat cgctatgtat ttggcacgtg agctcacaga taattcttta
    14881 ccgaaaatcg gagctgaatt cggtggcaag gatcatacaa cggttatcca tgctcatgaa
    14941 aaaatccagc agctgatgga ttctagtgtc agtatgcaaa atgaagtatc agaaatcaaa
    15001 aactttttac taaattaatt tgtcgattct atcaactgaa aatagcatgt ggataaaaga
    15061 aaaaaacgtt caaaagttat acacactttt tccacagctg ttttttcgtt tctctcttac
    15121 gttttttgag ttttccacag aatcaacatg ccctattact tttattattc tttttaacta
    15181 ataataaata aacaaacatt caaaggagat acatcatgaa agttacttta aaccgagcta
    15241 gctttatgca ggaattgcaa actgttcaac gagctatttc aagcaaaacc acgatcccta
    15301 ttttgacagg tgtaaaaatc acactgacac aagaaggttt gactttgacg gggagcaacg
    15361 ctgatatatc aattgaaact tttttgtctg ttgaaaacga aaaagcaaat atgcaaatcg
    15421 aatctactgg ttccattgtt ttacaagcac gtttctttag cgaaatcatt cggagacttc
    15481 ctgaagaaac atttacttta gaagttttag aaaataaaca agtagcgatc acttctggaa
    15541 aagcgaattt tatcgtaaat ggattagatg cagataacta tcctcatctt cctgttgtcg
    15601 aaagccataa ccagatgaaa ttacctgtac acgtattgac taaactaatc aacgaaacag
    15661 tttttgctgt ctcacaacat gagagtcgtc caatcttgac aggtgtgcat ttcattttat
    15721 ctgataattc tttattagct gtagctactg attctcaccg tctaagtcaa cgcgtgattc
    15781 cagtagaaca agcggctgat cattttgata ttgttattcc tggaaaaagt ttgatcgaat
    15841 tatctcgctc attaacaaat gaagaagaaa tcgtcgaaat cagcattatg gaaaaccaag
    15901 tgttgttcaa aacagaaacg atgtatttct attcccgttt gctagaagga aactatccag
    15961 ataccaatcg tttgattcct tcaagtttta atacagaggt tgaattttct gttcctagct
    16021 ttttagcggc gatcgaacgg gcttctttac tttctcatga aggccgtaac aacatcgttc
    16081 gtttatctat tcgtccagat gcggttgttt tatacggaaa ttcgccagaa atcgggaaag
    16141 tagaagaaag cttgagttat acagcaagca gtggcgatcc attagatatt tctttcaatc
    16201 cggattatat gaaagcagcg ttgcgtgcat ttggagatat gagcatcaaa gtgaaattta
    16261 tctctgctat tcgtccattt acattggaac caacggaaga tggcgttcaa tttattcagc
    16321 tgattactcc tgtacgtaca aattaacaat gattttcaat gttcttgaaa atttaattga
    16381 aaagaaatag aaaaaataaa attttaaaca gaaggcccta aaatctcttt taaggctttc
    16441 tgtttatttt tgtttaaatc acctttttta gattaaatcg tcttatatgg aaaaatatat
    16501 ggataaattt gttttattgc gaaaaaaaag gtataataga gtatactttt aataatgaaa
    16561 atgagggatg ggcattgaaa caacaagtca ttctaaatac ggaattcatg acgttgggcc
    16621 aaatgttgaa agaagtcaca gttatcggta gtggcggaca agcaaaatgg tatttggcag
    16681 aaaacactgt gttagtcgat ggcgaaccag aaaatcgtcg tggacgcaaa ctttatcccg
    16741 gcatgatggt tgaagttcca gaagtaggaa ctttttttat ggtaaaaaaa ggaaacgacg
    16801 ataatgaggc tgaataagct gtatttaaaa aattatcgaa attacgaaga gctgaacatc
    16861 gaattttcca agaacctcgt catcttttta ggagaaaatg cgcaaggaaa aacgaatctt
    16921 ttggaaagta tctacgtatt agctatgacg cgaagtcatc ggacaagtag tgagcaggaa
    16981 ttgattggct ggactgacga tcaggcgatg attcaaggag agatcacaaa aggttcatcc
    17041 actcttccat tagaaatctt attatctaaa aaaggccgaa aaaccaagat caaccatatc
    17101 gaacagaaaa agttaagtac ttacgtggga caaatgaatg tcattttatt tgcaccagaa
    17161 gatctttctt tagtaaaagg cagcccgcag accaggcgga agtttttaga tatggaatta
    17221 ggccagatca atccaatcta tctttatgat cttgtccaat accaagcaat tttgaaacaa
    17281 agaaaccagt acttgaagca attgaatgaa aaaaaacaaa ctgatttgct ttatttggat
    17341 attttgtccg aacaactagc agcgtttggc agcaaagtat tgaaagcaag agcacagttc
    17401 gtcaagcgat tggagttttg ggctaattct ttgcatcagc aaatcaccca tcaaaaagag
    17461 cagctggaaa tcgagtatct caccgcagtc gattctttag aaacacatac gcaagagcaa
    17521 atccaagaac aatttctcgc acttttgaac caaaataaaa agaaagacct ttttcgggga
    17581 acaacgacag ttggtcctca tcgtgacgat ctttcctttt ttattaatca gaaaaatgtg
    17641 cagacttatg gatcgcaagg acagcaaaga acaactgcac taagtgtcaa attagcagaa
    17701 atcgatttga ttaaagaaga gacaggggaa tatccaatcc ttttgttaga tgatgtgatg
    17761 agcgagctgg atgataatcg tcaactccat ctgctagaaa caatcgaagg aaaagttcaa
    17821 acgttcttaa caacaacgac acttgaccat gtaaaaaata aaatgactgt tgaacctgag
    17881 attttttatg ttcaacaagg gaaactaaaa ggagaagaaa ctgaatgaca gaagaaagaa
    17941 gtttagtaga acgcgctaaa gagtatgatg ccagtcagat tcaagtactt gaaggacttg
    18001 aagccgttcg taaacgacct ggcatgtata tcggttcgac tagttctgag ggtctgcatc
    18061 accttgtttg ggaaattgtc gataattcga tcgatgaagt attagcaggt tttgcaacga
    18121 aaatccatgt gattattgaa aaagacaaca gtatcaccgt tattgacgac ggtcggggga
    18181 tacctgtaga tatccaggca aaaactggac gtcctgcagt tgaaacggtc tttactgttt
    18241 tacatgctgg aggaaaattc ggcggtggtg ggtacaaagt ttctggtgga cttcacggag
    18301 taggatcttc tgttgttaac gcgctttcta cacagcttga tgtcaaagta tacaaagaag
    18361 gaaatgtgta ctatcaagaa tatcgtcgtg gagctgtagt ggacgactta aagattatcg
    18421 aacaaacgga tcgccatgga acaacggttc actttacgcc agaccctgaa atttttacag
    18481 agacaactga gtttgatttt tcaaaattgg ctacccggat ccgtgaactt gctttcttga
    18541 accgcgggat gcgtatatcg attgaagaca aacgagaaga agaaccagtc atcaatgaat
    18601 accattatga cggcgggatt aaaagttatg tcgaatactt gaatgcaaac aaaaccgtga
    18661 tttttccaga acctgtatat ttggaaggtg aacagcaaga tatcgcagtt gaagtctcta
    18721 tgcaatatac agacggctac cattccaata tcatgagttt tgccaacaat atccatacgt
    18781 atgaaggcgg aacacatgaa tcaggattca agactgcttt aactcgtgtg ataaatgatt
    18841 atgcccgaaa acaaaagctg atgaaagaaa atgacgacaa cttgaccggt gaagacgtgc
    18901 gagaaggatt gacagctgtt atttctatca agcatcctga ccctcaattc gaaggacaaa
    18961 caaaaacaaa attgggcaat tccgaagtac ggacagtcac tgatcggtta tttagcgaac
    19021 atttcatgaa attcttgctg gaaaacccaa gtgtgggccg tcaaattgtt gaaaaaggat
    19081 tactagcttc cagagctcgt ctagcagcga aacgtgcgcg tgaagtaacg agacgtaaag
    19141 gtgctttaga gattagtaat ctgccaggaa aattagcgga ctgctctagc aacgatcctg
    19201 aaaaatgcga attgttcatc gtcgaaggag attcggccgg cggatcagct aagcaaggtc
    19261 gtaatcgaga gtttcaagcc atcttgccta ttcgtgggaa aatcttgaat gttgaaaaag
    19321 caagcatgga caagatcttg gcaaatgaag agatccggtc tttatttact gctatgggta
    19381 cagggtttgg agaagatttt gatgtatcga aagctcgcta ccacaaatta gttatcatga
    19441 ctgatgccga tgtcgacggc gcgcatatcc gtacattgct gctgactctt ttttatcgtt
    19501 acatgcgtcc tattgttgaa gctggatacg tctatattgc tcagccacct ttatacgggg
    19561 taaaacaagg gaagaatatt acgtatgtac aaccaggaaa aaatgcagaa gaagagttga
    19621 aacaagtcgt tgcttcactt cctgcctcac caaaaccaag cgttcagcgt tataaaggac
    19681 ttggagaaat ggatgatcat caactatggg aaacaactat ggacccaagc aatcggatga
    19741 tggcccgcgt cagtgtggac gatgctattg cagcagatca gatatttgaa atgttgatgg
    19801 gggatcgagt agaaccccgt cgtgcattca tcgaggaaaa tgcacactat gtgaagaact
    19861 tagatattta atcatgaagg aggaaaaaga atgagtgaag aaatcagaga aaacatccat
    19921 gatgtcaatc taaccagcga aatgaaagac tccttcatcg attatgcgat gagtgttatt
    19981 gtcgcacgtg cacttcctga tgtcagagat ggtctgaaac ccgttcatcg ccgtatttta
    20041 tacggcatga acgaattagg tgtaacaccg gataaagccc ataaaaaatc tgccagaatc
    20101 gtcggagatg tcatgggtaa ataccatcct catggagact atgcgatcta tgagtcaatg
    20161 gtacggatgg cacagccatt cagctaccgt tatatgctag tggatggtca tggaaacttt
    20221 ggttctgttg acggtgacgg tgccgccgct atgcgttata ccgaagcacg gatgagcaaa
    20281 atcgctacag aaatgctgcg tgatatcaat aagaacactg ttgatttcca aagcaactat
    20341 gatgatacag aaaaagaacc agttgtacta cctgctcgct tcccgaatct tttagtgaac
    20401 ggaacaacgg ggatcgccgt aggtatggcc acgaatatcc cacctcataa cttgtctgag
    20461 gtagtagcgg ctatcgattt gctgatggat aatcctgatg tgacgaccaa tgaattgatg
    20521 gaagtattac ctggaccaga ttttccaaca ggtggtttgg ttatgggaaa atcaggaatc
    20581 cgaagagcgt atgaaacagg taagggttct attaccgtac gtgcaaaagt tgagattact
    20641 gagatgccaa atggtaaaga acggatcatc gtgacagaat tgccttatat ggtcaataaa
    20701 gcaaaattga tcgaacggat ttctgaactg cacagagaca agcgtatcga agggatcaca
    20761 gatttgcggg acgaatcttc ccgtgaaggt atgcggatcg tcattgacat ccgtcgtgac
    20821 gccagcgcat ctgttatctt gaataactta tataaattga catccttgca aaattcattt
    20881 ggattcaata tgttggcgat cgaaaaaggt gtgccgaaag ttttgagctt gaaacaaatt
    20941 cttgaaaact atatcgaaca tcagcgcgaa gtcatcacaa gacggacagt ctttgaaaaa
    21001 gaaaaagcag aagcaagggc acacatttta gaaggtttgc gtattgcttt ggatcatatc
    21061 gatgaaatca ttgcgattat ccgcgggtct aaatcagatg acgaagcaaa agcgacgatg
    21121 atcgaacggt tcgatttatc cgatcgccag tctcaagcta ttttagatat gcgtttacgc
    21181 cgattgaccg gtttggaacg ggaaaaaatc gaaaatgaat accaagaact cttaaaactg
    21241 attgaagatt taactgacat tctggcaaga ccagaacgtg ttacagaaat catcaagaat
    21301 gaattagcag acttgaatca acgatttggt gataaacgac gcacagaatt actagtcggt
    21361 gaggtcttaa gtctggaaga tgaagattta atcgaagaag aagaaatcgt tattacattg
    21421 acaaacaatg gttacatcaa acgtttggca aataatgaat tcagagctca acgaaggggt
    21481 ggacgtggtg tccaaggaat gggtatccac aatgacgact tcgtcaaaaa tctggtttct
    21541 tgttctaccc atgatacatt gttattcttt acgaataacg gaaaagtgta tcgggcaaaa
    21601 ggatacgaaa ttccagaata cgggcgcaca gccaaaggaa taccaattat caatttacta
    21661 ggtatcgatt cgagtgaaag catccaggca atcattgcgg tgacgggaga agcagaagaa
    21721 ggccactatc tcttctttac gacgcgtcaa ggtacagtaa aacgtacttc tgtcactgct
    21781 tttgctaaca tccgaagtaa tggtctgatc gcaattggtt tgaaagaaga cgatgagctc
    21841 gtcaatgtgc tactgacaga tggtcaatct aatatcatta ttggaacaca taacggctac
    21901 tctgtcactt tcaaagaaga agcagtccgc gatatgggac gtacagcttc cggggttaga
    21961 ggaatacgcc tgcgtgagga agattatgtc gttggggcag ccttaatgaa ggaagatcaa
    22021 gaagtattag tgatcacaga aaaaggttac ggaaaacgga caaaagtatc ggaatatcca
    22081 gtgaaaggcc gcggaggtaa agggatcaaa acggcgaaca tcactgaaaa aaatggtcca
    22141 ctagctggat tgaccactgt ttctggagat gaagacatca tgctgattac agataaaggt
    22201 gtcattatcc gtttcaacgt atcgactgtt tctcaaactg gacgttcaac attaggcgta
    22261 cgtttgatga agatggaagc tgatacgaaa gttgtcacga tggcagcagt cgaaccagaa
    22321 gctgcagaac aaacagttga agaacaagga tcagaagaat agccatcaag actaattgtg
    22381 taagctagca agaatgaccg attaaaaaat ctggttattc ctgctggctt ttttgattag
    22441 ttgaatttct cttaaaatat tcgaaactgt tcattctgta ttgcttttga ccaaaataga
    22501 gtgtataatc agagcttgtg agtagtctaa ttaattagat aactctcctt gctcttagga
    22561 acctaagagc cgaaagtcca cagggaggtg aatatcaatg gaaaacacga aatatgaaat
    22621 catgtacatt attcgtccaa acattgatga agaagcaaaa actgctttaa tcgaacgttt
    22681 cgatgcaatc ttgaaagata acggagcaga agttatcgaa tcaaaagatt gggaaaaacg
    22741 ccgtttcgct tatgaaatga acggttaccg tgaaggtatc tatcacatcg ttaaagtatc
    22801 ttctccatca tcagcaaatg ctgtcaatga atttgaccgt cttgctaaaa tcaacgacga
    22861 tatcattcgt cacatgattg ttaaagaaga agcttaattg ttgtttcatg tgaaacaata
    22921 gaaaacgatg agaggagatg actattttga ttaataatgt tgtattagtt ggacgtctga
    22981 cgaaagatcc agatttgcgt tacacagcaa gtggtacggc agtcgcaaca tttactttgg
    23041 ctgtgaatcg taattttaca aaccaaagcg gcaatcgcga ggctgatttt atcaactgtg
    23101 tgatttggcg caaatcagca gaaactttag caaactatgc tcgtaaagga acacttttag
    23161 gtgttactgg acgtatccaa actcgttctt atgataatca gcaaggacaa cgtgtatacg
    23221 tgacagaagt agtagctgac aacttccaat tgctggaaag tcgttctgca tctgaaaatc
    23281 gccaacaaag cggcggattc caaagttctg gtcaatcagc tggcggattt ggcggaaaca
    23341 ataactctaa tcaaacatct caatcatcca acggtatgcc tgatttcgac cgtgatactt
    23401 ctgacccatt cggctcaagt tcaacgatcg acatttcaga cgatgattta ccattctaaa
    23461 cgaatttgat aggagggaaa tagaatggca caacaaagaa gaggcggacg taaacgtcgt
    23521 aaagtagact acattgctgc taaccatatt gaatatatcg attataaaga tatcgaatta
    23581 ctaaaaagat ttatttcaga acgcggtaaa atcttgccac gtcgtgtaac tggtacaggc
    23641 gctaaaaacc aacgtaaaat tactgttgca atcaaacgcg ctcgtatcat gggcttgcta
    23701 cctttcgtta gcgaagacta ataaatccta ggaattgaat gactgatcct tttgatcagc
    23761 tattcaattc ctttttttct gtaaaaataa cctctattcg tttagtcgct cgaatgacga
    23821 aagttatggt aaaatcaaat tagtcatata tgggaggaaa attatggaaa ataaaaacaa
    23881 tcatttatcc ccgaaacctc tcttccttat cctgtttata ttaatcgaag tattggtagt
    23941 cttaatttta ccatttcgtt ttgttagcgc ttcggtcatt ctattgatga atttatgggg
    24001 tgttcgctat tggttgaaaa tgaaacgttg gcttatttta aataaacgtg aaaaaatcca
    24061 gcaggcttcg actgtagctg aaaccaatct agcttatgtc tcagaagtca tgcctgtagg
    24121 agtgattgct tatcagccat caagccaaaa aatcgaatgg ctcaatccat tagcattgaa
    24181 aatcaaagat caaagtggat taacagagga agaattgcta gatacttttt ttactgcaaa
    24241 agaaaaaaag aaaaaccaag tctatctagc agataaaaca tttcagtttg atatggatac
    24301 agtaaagggt gttgtttatt ttaatgatat tacccaaaac cagttgttaa aactccatca
    24361 gacgaacagt cagccggtga ttggtatttt atctattgat aactatgcag atgccattga
    24421 taaattggat gacaaagaga tttcttattt gaactctttc gtcactacac tagtttctga
    24481 ctggatgaat gaataccatg tctttttcaa aagaatcaac gcagaacggt ttttcttcgt
    24541 agcacagatg atcgatctga aaaagatgat ggataaaaaa tttgatttgc tggatcgctt
    24601 tcgagcagaa gctgaaaaac agagctcgcc tatcaccctt agtatgggga ttgcttatgg
    24661 ctcaagaacc ctcgatgaaa tcggtggaga agctcagaat aatttagatg ttgctttagt
    24721 ccgtggaggc gaccaagttg tcgtgaaaga ggtaaaagaa ggagcaaaac ctgtttatta
    24781 tggggggaaa tctgctaaga atgtcaaacg cacgcgtgtt cgttctcgag cgatgagtac
    24841 cgctttgcgt cggatttttg atgaaacagg cgatgtgttt atcatggggc acaagtttcc
    24901 ggatatggat gctttaggct ctgcttttgg tgtcagttgt ctggcacgct tcaaccagaa
    24961 aaaagcttac atcgttatca acgagaacga gatcattcca gatgtagaac ggtgtcttga
    25021 agaaatccat aagcatcctg aattagaaat gcaactaatc tctccagaac gtgccataga
    25081 gctgaaaaaa gaagaggacc tcctcgtgat ggtggattac cataaacctt ctctatcgat
    25141 ctctcagggc ctatatgagc aatttgataa gattgtgatc attgatcacc atcgtcgtgg
    25201 ggaagagttt ccaagtaaac ctctgctgac ttatatcgaa tcttcagctt cttcagcttc
    25261 agagctagtt tctgagctga tccaatacca aagcagcaaa acaaatcgct tagatcgttt
    25321 tgaggccaca ttattgcttg cagggatggt tgtagataca aatagtttca atgtccgtac
    25381 aactgcacga acttttgacg ttgccagcta tttaaggtct tgtggagcag acgcatcctt
    25441 agtacaatat ttattgagct cggatttagc gacatacttg gagatcagca atttgattgc
    25501 tggaagcgac tatgtgactt ctgatattgt tgtggcggca gggacagacg aaaaagagta
    25561 taatagtgtt atagcagcaa aaacagcaga tacattgctt tcgatgatca atatcaatgc
    25621 agctttcgtc attacaaagc ggacagacgg cttgattggg atcagtgcca gaagtaccgg
    25681 cacgatcaac gtccaaatca tcatggaatc cttaggtggc ggaggacact tcacaaacgc
    25741 agctactcaa ctggaaaata cgacagttga ggaaacaaaa cagaagctat ttgctgcaat
    25801 caaagaaaat atgaatcaaa tatatgataa ggagtaatcc aaatgaaagt aattttttta
    25861 caagatgtaa aaggaaaagg taaaaaaggg gacatcaaag aagttccagc aggttatgca
    25921 caaaattatc taatcaaaaa taacttggca aaagaagcaa cgaaaagcag tatcagtcag
    25981 ttagcaggac aaaagaaagc acaagaaaaa cacgaagctg aaatcttgga agaagctaaa
    26041 aagttaaaag aattcttgga aaaagaagat actgtagtcg aattgaaggc gaaagcaggg
    26101 gaagacagcc gtttgtttgg ttcgatccct tcaaaacaaa ttgctgaagg gttaaacaaa
    26161 caatacaaaa tcaaattaga taaacgaaaa atcgaattag aaaatccgat ccgttcacta
    26221 ggctacacaa acgtacctgt taagcttcat catgaggtga cggctacgat caaagttcat
    26281 gtcgtacaag aataacaaat cttgatagcg atgttcagct ggtaagtata accagaagga
    26341 aaaaagacaa accgaaattt atctgagaaa acagctgcag caatgaaagc aaaagctgca
    26401 aatcgatttc agattgattt cggtttcttt tttgggaaca gcaaaatagc ataaagaaaa
    26461 cgagtataat aaaagagaac tattttttta ttacactttc ggtacgaatg atgacttttc
    26521 tctcattttc ttgtaaaatg aagcaattga agacaaggag cgatacgtat gaatgaagtg
    26581 tggcaggatc gaatcccacc gcaaaatatt gaagctgaac aagcggtttt aggctctgtt
    26641 tttcttgacg cagaaacgat cattgatgcc atggagtaca tagagccaaa agacttttat
    26701 cgtcgtggcc atcaaattat ttttgagaca atgatcgagt taaatgatcg taatgaagcg
    26761 attgacgtgg ttactgtcaa agaccggctg gaacaagcca atttgttaga agatgccggt
    26821 ggtttaagtt atctctcaga cttggcatta gcagttccga cagcagcaaa tatcgtctac
    26881 tatgcgaaaa tcgttgaaga aaaatcattg ctgcgtacat tgatccagac agcaacagga
    26941 atcgttacga aaggattcga gcaaggagaa gaagttcaaa caattttaga tgatgccgaa
    27001 cgcagtatct tggaggtttc ggaaaaaaga aatcgaagcg gctttttatc tattgctgaa
    27061 gtactgaact ccacgattga aaatatcgat cgactagctc aaaacaatga agaaatcaca
    27121 ggtctaccaa ctggctatca aggtctggat aaaatgacag caggtttcca gcccgaagaa
    27181 ctaatcattc ttgctgcacg tccagcggta ggtaaaacag cgtttgcatt gaatattgct
    27241 caaaatgtag gaacaaaaac agatcgttct gttgcgatat ttagtttgga aatgggggca
    27301 gaatctcttg taaatcggat gctttgtgca gaaggttcga ttgaggccag ccatttacgt
    27361 acaggtcagc tttcggaaga agaatggtcg aatctgatcg ttgcgatggg gagtctttct
    27421 cgagcaaata tttttatcga tgatacacca gggatcaaaa tctctgaaat ccgtgcgaaa
    27481 tgtcggaaac tagcgcaaga aaaaggcaat ctaggcttga tattgatcga ttatttgcag
    27541 ttgattgaag gtaacggtaa agagaaccgt caacaagaag tatcagatat ctctcgccaa
    27601 ctgaaaaaac tggcgaaaga gctgaaagta cctgtcattg ctctttcaca gttatctcgt
    27661 ggagtagaac agcgacaaga caaacgacca gtgctaagtg atattcgtga atccggatca
    27721 attgaacaag atgcggacat cgtagccttc ttgtatcgag atgattatta tgaaagagaa
    27781 ggcggagatg acgatgatgg aaacgagcca aaaaatgaca acgtcatcga agtcatcatc
    27841 gagaagaacc gaagcggtgc acgaggaaca gttgaactac tgtttatcaa agaatacaat
    27901 aagttttcgt cgctttcgcc aagagaagaa ttttaaagaa gaggttgtga gccttgagca
    27961 atcccgagag ctctcgtaag aagatgcaag gagtaaccgc aggaacgact agagtactta
    28021 atttaaacag atctccaaag ggattgtgac aaaagtcttg tcacagtccc tttttccgaa
    28081 taaacggtgt tcaatcagct gacccttggt attagctcag caaacgtcaa aatatgagga
    28141 actattttga cgtttgtttc tctaatactc aggtcaaaac gctgatctta cgacctcttt
    28201 tccttaaacg gtgttctcct gccaactcct cggtcacaag tcacaatatc atcaaacatg
    28261 agaagcagtt ttttgaaatt gctccttgtg actcagagtt aaacggcagg ctcacaacct
    28321 ctttcttaaa cggtgttcaa aagcttgcat ctgcggtaat acgcccaatc aaacgaaaaa
    28381 tatgagaagc tatttttgtt tggctgttct tattacttga tgcaggacag ctttttcaca
    28441 acctctgatt cacggtgttc caaaagtagc ttcttcgaaa aataagtcga aatttcacaa
    28501 aaatttgaga agcaattttc gtacattcct tcttatttct tgaagctgac cacttctgtt
    28561 acaacctctt ttttaataac cattctaaaa agataaagtt cgttttttaa gcgtttttat
    28621 aaaataaaaa taaaaatcat tcgattttat ttgaaatatg tatctattat tgatacaatg
    28681 gttcaggatt aaataatata gaacgaggtg ttcacatgtc atctgtagta gtagtaggta
    28741 cgcaatgggg cgatgaaggg aaaggaaaaa tcacagactt tctcagtgaa aatgctgaag
    28801 tgattgcacg gtaccaaggt ggcgacaatg cagggcacac aattaagttt aacggtgtga
    28861 cttataaact gcatttgatt ccttctggga ttttttataa agacaagatc agtgtgatcg
    28921 gaaacggcgt tgtagtcaat cctaaatcat tggttaaaga attagcttat ttacatgaaa
    28981 aaggtgtaga aacaagtaat ttgcgtattt ctgaccgcgc gcacgtaatc ttgccttatc
    29041 atatcaaatt ggaccaatta caagaagatg ctaagggcga aaataagatt ggtacaacga
    29101 ttaaaggaat cggacctgcc tatatggata aagcagctcg tgtaggtatc cgtattgcag
    29161 acttattaga taaagaaatt tttgaagaac gtcttcgttt gaatctagat gacaaaaacc
    29221 gtcaatttgt aaaaatgttt gattcagaac cacttgattt tgacgatatt tttgaagaat
    29281 attatgaata cggacaacaa atcaaacaat acgtcacaga tacttccgtt atcttgaatg
    29341 atgctttgga tgcaggcaaa cgcgtgttgt ttgaaggcgc acaaggtgta atgctggata
    29401 tcgaccaggg aacttatcct tttgttactt cttctaaccc agtagccggt ggggtaacaa
    29461 tcggaagcgg tgtagggcct tcaaaaatca ataaagttgt cggcgtctgc aaagcctata
    29521 cgtcacgtgt cggtgatgga ccatttccaa ctgaattatt tgatgaaaca ggagaacaaa
    29581 tccgcgaagt aggacgagaa tacggtacga caactggtcg tccgcgtcgt gtaggctggt
    29641 ttgattctgt agttatgcgc cattctaaac gagtttcagg tattaccaac ctatctttaa
    29701 actcgatcga tgtattaagt ggattggaaa cagtaaaaat ttgtacggct tatgaattag
    29761 atggcgaact aatctaccac tatccagcaa gcttgaaaga gttgaaccgc tgcaaaccaa
    29821 tctatgaaga attgccaggc tggtcagaag acattacagg atgcaaaaca cttgcagaac
    29881 tgccagaaaa cgcacgtaac tatgtacgtc gtatctcaga gcttgttggt gtacgtatct
    29941 caacattttc tgtcggacct gatagaacac aaacaaacat cttagaaagc gtctgggcgc
    30001 aaatctaagg ataaaaacga cgactgaatc aggacgttct taccagagcg agtcgaagtg
    30061 tttatctata cgataataat aacttgaaca ctgtttattc agaataaggg actgtgacaa
    30121 aaaccgtgtc acagtccctt tttccgaaca aacggtgttc aaaagcttgc atctacggca
    30181 ctaagcccaa tcagatgaaa aatatggagt gctattttta tctgattgtt cttactgctt
    30241 gatgcagagc agctttttca caacctccta catggtgttc aaaatattat tcgccttttg
    30301 cttctttcac taattttgcg aatgcttgtt gttggaattc agcagctaag gctttcgtac
    30361 gttgttcttg ctctgctttg ctcatttttg gattttcagt cattgctttt accataccgt
    30421 cttgaacata agcttttgct tcactttcca tcgtactttg ctgtttcttc actaattcag
    30481 ctaacttggc tgcttggtcc gtacttaatt ctacccatgt ctctgggatt ttgaatgtat
    30541 tcactcggct gtcatattcg tgattgttat cagcaatatt gaaccagaat ttatacatgt
    30601 cgtttttgta ttcccaataa gtagttttcg tcactttttc agcatcgccg tctgtctttt
    30661 ctactttatt tgtttcgtta tccgtaccag ttgcttttgg tttttcttgt ttttcatttg
    30721 ttcggtaaag atacactttt tcttttccat tccctaaagc tttatacaaa agcatatctg
    30781 ctccttggga atcagcactg gaaacaattt tttctgtttt tgtttccgtt actttttcca
    30841 ttccaaagtg attggacaaa ttagcgacga tcaaacttag tgaagcgacg aagattagtc
    30901 cgaaaatcaa agataaaaag gtttgccatg tttttttagc gaacacgttc gttaaagcaa
    30961 aagcaagcaa gctgacaatc aaaactccaa taatcatgca ttatcccccg ctttcattag
    31021 aacaggttct tttttcttca ataaaaaggc aataataaag ccaagaatcc caaagatgat
    31081 cgctacgata aacgcggcat ggtaacctga cagtgtggca ttgattgcct gatctttata
    31141 agctaatggc gtattatcca atacagattt gcttgggata tttgcttttg tcacattcgt
    31201 caacacactg atcaaaatag ctgtaccaat cgagcttgcg atttgacgga atgtattatt
    31261 taccgctgtc ccatgactga tcaagttatt tggcaatgca ttcataccag aagtcgttac
    31321 cggcatcatg accatggaga tcccaaacat acggatcgca taaaggatca cgatatataa
    31381 aactggagtc gtttttgtca agaaagcaaa tggtgcagtt gccgctgtca aaatgaacat
    31441 ccctgtgatg gctaaacgtt tcgcgccgta tttatcgaaa atcgcacccg tcacaggcat
    31501 catagccccc atgatcaatg cacccggcaa caacatcaag ccagaatgga atgctgattc
    31561 tccacgaata ttttgaatat acaatggtac gaccatttct gcccctacca tcgccatatt
    31621 tgttacacca gctaaaacag ctgcaatcgt gaatgtagat gatttgaata cacgaagttc
    31681 caagaatgga tgttccaaat gtaattgacg ccatgagaac aaggcgatga aaatcacacc
    31741 aacaactaag aaaccaatta ctttcaagct tccccagccg tcatctccta cactagaaaa
    31801 tccgtaaagt aaactaccga atccaatcgt tgataaaata gcggataaaa tatcaatgct
    31861 aggatttgac aatggaataa cacttttcat taggaagaat gctaaaacca agacaagaac
    31921 aacgattggc agtaccatac caaacaagtc acgccaatgg tagttatcaa tgatataacc
    31981 agataatgta gggcctaaag caggagcaag tccaataacg atccctgaca tccccattgc
    32041 tgatcctctt ttttctggcg ggaaaataga tagcatgata ttttgcaata atggcatcga
    32101 tactcccacg ccgcaagctt ggatcaagcg accgattagt aatgtactaa aattaggtgc
    32161 tgtaaagcaa gtaatcgttc cgatcaaaaa gatcgtcatg gcagaaagat ataaatttct
    32221 tgaactgaat ttattgatca gccatgctgt tattgggatc atgatcccgt tgactagcaa
    32281 gaaaccagtc gttaaccatt gcacatcaga cgctgaaata tcgaaagctt tcatcaatgt
    32341 aggaaaagct gtcgtcaata aggtctgatt caaaacggta caaaatgtac caattaataa
    32401 gacaacaact aaaagtgtcc gattatatgg ttttccgtaa atatctacgg gttggttgtt
    32461 actcatattt ctccttcttt ctgaattttt tctgaaacta ctagaatact ttagtcctaa
    32521 attcgctatt tgtcaaccta cttgacacat aagttaatac ggatataatt aagggaagca
    32581 agaggacaaa aggagtgaca aaaatggttg gggcttccct taaacgtttg ttggaaaatg
    32641 atcaattggt tgttggcatc agtgaattaa gtgatatcgt aggtgtgtct ccacgtcaat
    32701 taaggtattg ggaacaaaag ggattcattc gttcgatcga agctgatgcg aattgtccta
    32761 gaaaatatcg tttacctaca gtcataaaag tagaactgat caaaaaatat ttagatgacg
    32821 gatttacttt gtcaaaagct gcagaaaagg cagaaatccg ccaaaagaaa atgcaccatg
    32881 tccgtaaagt tttttcaaaa gcaatcaaag atatcgaatt gatcgacgaa cgctatacga
    32941 ttttacaaat cggtaacttc aaagaagaga atgctaagat gtacatcatt catgacaacg
    33001 agacagacga attaacttac aagctccttc ctgtcgatga aaaagctgac tacgaacagt
    33061 tgttaaaaga actctaaacg ccactaatct cctaagtacc aagcttttta ataagtagat
    33121 gctacataaa tacttgaaaa aaggcctgtt tgaacataag aatatccgaa cggtgatctg
    33181 cgtttctttc atatagaaag gaattctcca tcactgttcg gatatttttt tatttttttc
    33241 ggttactttc ttctaataca tctgttagcc aatttgcata ttctgtttcc cgattgatcg
    33301 catgcgctaa aatcaggtag tgtccgtaat tttcatgttt ttctttttca gtaggaaaga
    33361 tcgttttttt tcgctctaat agatgaagtc gtttttcttc atgaaggtca cgctgctgct
    33421 gaataatttc tactaatgcc ggatcattca cgtctttgac gaaatacaat ttcagaacaa
    33481 attcgtctcg attaaccggt agttcattgg ttggtgtatg aatccactct gccaattgtt
    33541 ccttcccttc ttctgttaat tcataaactt tcttttccaa tttttcacct gtgatctcta
    33601 cttgatgctg aatgattcct tgctcttcca tttttgcaag ttcaggataa atctgactat
    33661 gttttgcttg ccaaaattct cctatttcat ttttgaacga ttgattcaat tcatatcctg
    33721 tcatcttctt cttacttaat aaccctaaca aaatatatcg aagtgtattt tttcttgcca
    33781 tcagttttcc ctccttgaca aatctttaaa tccattctat cataaatgag taatcgaaaa
    33841 catgtagttt attacatgtt ttaatttata aggagatgct taaacatgaa agaatttaaa
    33901 gaattggaag attttattgg aactcacttt atctatacgt atgacaatgg ttgggaatat
    33961 gaatggtatg ccaaaaatga ccatactgtt gattaccgta tccatggcgg aatggtcaaa
    34021 ggccgttggg taaaagacca agaagctgat atcgttaaat tgacggacgg cgtattcaaa
    34081 atcacttgga cagagccaac agggacagac gtggcattgg actttatgcc aaatgaaaag
    34141 aaacttcacg gaatgatctt tttccctaaa tgggttgaag aacatccaga aatcactgtt
    34201 actttccaaa acgaacatat cgcagaaatg gaagcagctc gtgaaaaata tgaaacttat
    34261 ccaaaattac ttgttcctga atttgctacg atcacttata tgggtgatgc aggtatagac
    34321 aatgaagatg ttattagcga agaaccatac gatggaatgc cagatgatat ccgtagcgga
    34381 aaatacttcg atgaaaatta taaacgagta aaataaggtt gtgaaagaga ggttgtgaaa
    34441 tcagcgttct gacctgagta ttagagaaag aaacggaaaa atagttcctc atgttttgcc
    34501 gttttttgag ctaataccga gggtcagctg attgaacacc gtttatcaag aggttgtgaa
    34561 atcagcgtta tgctttgagt aataagaacg ctagttagaa aatagctttt catattttct
    34621 aggctagtgg gcttattacc gagaagcagc cgtttgaaca ccgtttatca agaggttgtg
    34681 aaatcagcgt tttgacctga gtattagaga aacaaatgga aaaatagttc ctcatatttt
    34741 gccgtttttt gagctaatac cgagggtcag ctgattgaac accgtttatc aaagattgta
    34801 acaactgcat attcacacag ttaaattagt gacttcaaaa tgaaataatg ctcccagcaa
    34861 aaaattaggc tgggagcatt atttcgttat tctgttcatc aaaaatcagc gttttgattc
    34921 catttcatca caagttcctc ctcatttact gcttcatctt tttgctgtgt ttgaattgta
    34981 aaaccttgtg aaagatagaa atgaatagct gattgatttt tttgatagac tgtcaaagtc
    35041 agtgttttat tggtttcttt tatttttttc attagttgcg taccgattct ttgttgccgg
    35101 tattcccttt tcacaaagat tcctgcgata aactgatcaa tcaccccagc aaaaccaata
    35161 atttcttgat ctatttctgc taagtaaatc actgcatctg gtaaagcttt cctaacagat
    35221 tctacttgat tctcccaata agttgatgga atgaattcat gagaatccaa gttagcatgc
    35281 agccaaatcg atagaacaga ttccagttct ttggcagaca attttgttgt tgtgcggatc
    35341 attttttctc tccttactta ttcattgatt ttaaaataaa aaccattttt ttgaagctct
    35401 tttacaagtt ttcgcccctc aaaaaagtat atcttctctt gttttacttt tctaatacca
    35461 aaagaaacag caactttagg atcatcagct gaaacgattc gataagttcc accaggcaat
    35521 tgatccagct tgactttccc atgatggtcg ctttgtactg aaaacggcac accatttcta
    35581 gtaagtgttt ttttcttatg tttcaaaatg aatgaactac catttaccgg tttccctgac
    35641 gaatcactaa taataaatga aaaatcaagc acttttttac ttaatctata ctgatagatt
    35701 agtcgtccga taaacactaa cagtatgact acagctatta tcgttcctgc aagaataaac
    35761 cattgccgca tggttctttc ctgatttcct tttccttctt ctttttttac cgattcgtta
    35821 taaggaatcc gatgtcctgt cactagcaaa cgatgcgtgt tgatcatata cggcgtacat
    35881 gtaagcaagg taactaagtc ttctccttct gaaatcgtta gaacagacgt gtcatctggc
    35941 agtaccactt gtatattttt aacttgataa gccaatttct cccccaaaac agtcaaaata
    36001 aacgtgtctc cgttttctaa acgatctaat tcggtaaata accgtcgatt aggtaaaccg
    36061 ctgtgagcag agatcactga atgcgtcgaa tttccaccaa gcgggaaaga ggttccttgc
    36121 aagacgcctg ctccattttc aaggatccta ccattgagcc gatcataaag tgaaatattt
    36181 aattggatct taggtatatt gatacttccg atcagccatt cctctaattg gctggatgtt
    36241 gtactttcat ttctgttctt ttcattgaaa ggatcagtac caggattgaa tccttggtca
    36301 gttaatcgtt gttttttctc ctccattttc tttcgctgca cttcaatttc agctgcggtc
    36361 cgtttttgta cttgttcgat acgtttttga tcaataaaac tatttaaaga atccacataa
    36421 aatggatata aagcgatcag taaccctacg acgatacata ttaataaaaa caaacgaaac
    36481 aaaagtcgac gagactgttt ttgcttactc tgcttcacat gtgtcctccc ttgcaagttt
    36541 ttttgttctt tcttttatta tatgggttca ggaaaaaaat agaaaatgaa agccgatgag
    36601 gttgtgaaaa agctgtcctg catcaagtaa taagtacagc ccaaaaaaat agctactcat
    36661 attttttgtt gggttgggct tattaccgca gatgcaagct tttgaacacc gtttaaggaa
    36721 gaggttgtga aaactgcgtt atgctttgag taataagagc gctagtcaga aaacagcttc
    36781 tcatattttc taggctagtg agcttattac cgagaagcag ctgattgaac accgtttacc
    36841 gagaggtcat gaaaaagcta ctttgtttcg agtattaaga aaaattctgt ttcaaaacaa
    36901 ggagatgcaa caaaacttat gtcgcatcct ctctccttag aactttcact tatgtttttc
    36961 ttgtaaaatt cataaatatt ttcatgaatt ttctaatttc ttgcctacct ctcgcatagg
    37021 atatatgatg aatttatcta aagtttgcaa aggagttcga caattgaaaa aatatggccc
    37081 atttttagca atttttttca ttgctttggc atttctcatg cctcatttat tcacacataa
    37141 actaattttg ggtgctgatg ccccattcca ttacaatcgg ttctatgatg ccgcgcaaca
    37201 aattaaatat ggggatttcc attattttcc ttccctttat ggattccagc aaagtggcag
    37261 gatcgtgaat gccgtttacg gcccattttt cgcttattta caaggattga tcgtgttgct
    37321 tgcaggtagc tggtttcatt atcagattct ttcaaatttc attctttatc ttatttcagg
    37381 attttctatg ctgattttac tgagatatgt caatgttcga aattgggtaa gtgtaccagt
    37441 agccgtttta ttcatgagta cgtattctat tcaatactgg tctatcaacc aaggattcac
    37501 tagctggggg acttgttttt atcctttatg tatgatccct attgctgatt tcgtccttca
    37561 taaaaagttc cctgttatca aagtcgctgt ctcagtggga cttatgacac agatccattt
    37621 actgtcttcg atcatgttag ctttgatgta tatccctttt tatctttatt acttttttaa
    37681 ccagactgaa aaaggaaaag cactgcttca actgattaag tcggtcttgc tttattttgt
    37741 tctgacagca aatatttggg caggattagc tattgtttac agtgggaata agattcttcc
    37801 tccctttgtt aacagagaaa tgagtcaaaa ggcgatagat ctggatggca gttattggct
    37861 ccattaccct aaggcctttc ctatattgat attatcaggt tttttgcttt ttattttctt
    37921 tcaacaaaag aacaccttat tccaaaaaat cgtgttgtac acaagtcttg ttttcttttt
    37981 tctttctact agcttgattc catggacgac attgatcgaa aaaaatgtgc cttttattag
    38041 tttgatccag tttccatttc gatttttcgt gccatttacg ctattgcttc tactttacct
    38101 tgccctttca ctgaaccagt ggtctgaaca aaagtggttc cgtcttttaa gtatatttgg
    38161 cattcttatt ttttcagcac agacgatcca gaacttatac cagcatctgg aaaagtggga
    38221 aaatgaaaca tttgtcagtc gacatactta tctattcgat acgccggaag aagcaaggaa
    38281 aacattcttc agcaaagata tgtctaccag cttgttcgtt ttccaaaaaa ccacacctga
    38341 ctatctgcca atctataagg agacaaaaga caataaatac gaccggtata acgaagaaat
    38401 actagaaaac gaaacaactt ttataaaaac acatccaggc ggtcttttga caatcaagtg
    38461 gaaaaacact ggggacaaaa ctgttcatat ccctgttatc gtctacgatc ggactgtgct
    38521 acagcaaaat ggaaaaacat tgactgatta tgaagtaaca gatatcggga ccccaatcgt
    38581 gaagcagaaa aaaggaatca acgagctcac gcttcattat caaacgccaa tctatttcta
    38641 tttcattctt tctcttactt tgattggctg gtttaccttg ctatgtttat ttatttatca
    38701 tagatacaag cttttacgtg cttaggcaaa agggataaat acatccgatc aaaaaactct
    38761 ccaaacggta taatcgtttg gagagtttta ttttaatcag tttttttatg ttatatgtta
    38821 tattttttat tcgttcacat ctaattcgat ttccaccccg tagtgatcac tgatgattgg
    38881 actatcgatc ccatcaaaaa tccgttgata ttttttaacc tgcattcctt cagggacgaa
    38941 aataaaatct atacgcaatt tttctgtatt ttgttcccaa ccatctattt tcttttccac
    39001 agtagctgct tttccttttt cattcgcgac aacatacgaa tcttgaagaa gaaggcaact
    39061 ttctcttaca gtataataac cttgagagtc tactggatta ttgaaatcac ctaggaaaaa
    39121 aagtggtacg cgagtttcca aaaagtagtt ttccaagttt ttccactcaa aataaaagcc
    39181 acttttttta tctgtccacc aagaaaagtg gcagctcgag acaaaaatgt cctgatcatc
    39241 tatctttgtt ttcccaatca agatcttgcg tgtctgataa tcttctttct tttgagattg
    39301 ggagacggta aggacctgac tttccaatgt ttttttcgta agcagtgcat ttccttcttc
    39361 atatttatca taaccgatgt ggctcatttc ccaactccaa aaatactctt ttccgtgttc
    39421 ctttaaaagc tgaactaaac ggtaggcaaa attatcttta cggataggta cttgattctt
    39481 tacctgacag aacttcatta gcttcgcagg ttctacttct tcagattcca gtaattggtt
    39541 gatttcttgt aaagcaatca cttcataatc ttctaacagg atcttatctg ctaattcttg
    39601 caattttttc tctggttctg gctcaagcca gctatgcgtg ttgagtgtca tgaatttcat
    39661 tggttttctc cttgtcttca tttcgaaaca aaagggttgt gacaaaattt ttgtcacaac
    39721 ctctccagca aatagtgttc tcctgctaac tcctcagtaa taagctcaat ggaccaggag
    39781 ctattttttg tttggctgtt tttatcactt ggtgcagggc agctttttca cgacctctcc
    39841 actaaacagt attcaaactg ctgcttctcg gtaataagcc caaccaaacg aaaaatagga
    39901 ggagctattt ttgtttggct gttcttatga cttgatgcag gacagctttt tcacaacctc
    39961 tccttacatt ttaatggagc cgatcacttt accagctgtc actgtaccta attgcttgat
    40021 cactacttct tctactttat cgtttgtaaa tacgatcatg atcgttggat ctttttcttc
    40081 gttttggatc acatcaagat ccatctcagc taatttcgtt cctgttccta cttcttggcc
    40141 ttctttgaca gcaatctcaa aagctggctg attcatttgg actgtatcga ttcccatatg
    40201 gatcaaaact tcgataccat ctttcgtttc cagcccaatt gcatgtttgc tagggaaaat
    40261 acttgtcact tttccagcga tcggcgcaac aacttctctt gatgttggaa taacagcaaa
    40321 gccgtcaccc atcatttttt gtgagaatac cggatctttt acttcagata aaggaatcac
    40381 ttctccagaa gctgcggaaa ctagttcttg ttctatacct aagtattgct tactacctgc
    40441 ttgtccattt cctgtagaaa ccatttctgg ctctggaatc tctgctcctg attgcagcag
    40501 atcttcgata tctgatttca agacatctgc ttttggtcca taaactgctt ggactccatt
    40561 gtctttgacg atcaatccca tcgcccctgc tcgtttccag gcttcttcag tccctacttt
    40621 ttctcgatca tttacactta cgcgtaaccg agtcatacaa gcatcgacat ctttgatatt
    40681 ttcttttcct cccagcaaat gaatgatctg gaccacttgc tgatctgctg ttcctgttcc
    40741 gctgttagat acaacggttt cttcactgtc attgtcgtaa tttccgttac gtcccggtgt
    40801 agcaaagttg aattttttga tcaagaaatt tgctaagaaa tacgtaacaa caccaaaagc
    40861 gatcacacac aacacgaagt tgataagatc tccacctaga cccgctttga tagctaacgg
    40921 tgtccgagtc agcaattcga tattcccaaa tgaatggacc cgcaatggca agatatctgc
    40981 catcgcaaat gcagcccctt ggatcactga ataaatgaca tataatggca cagcagcaaa
    41041 cataaacatg aactccaatg gttctgttac acctgttaga aagacagcca acgctgctga
    41101 aaaatacatt gatttatatc ttgctttctt atcaggatcg acattacgat acatcgcaaa
    41161 agccattccc atcaagatac ctgaagaacc aatcatttgt cctactttga aacgagccgg
    41221 cgtccagtta tgcaacacaa actcatattg cgacatatcc cctgcacctt taagattcac
    41281 taaatcagtg gcccaagcca accataacgg atcttgccca aatacttgtg tccccgcctg
    41341 tgcgcctgat aaaatctcat acgatccgcc aagctgtgta tagttgatag gaatcgtcag
    41401 catatgatgc aatccaaatg gcaatagtaa acgttctaat gttccaaata aaaacggtgc
    41461 tagaatcggt gcgctttctt gtgattctgc aatccataaa ccaaaattat tgattccagc
    41521 ctgtacgttt ggccacacca cagcaagaat caatgccacg attgttgacc ataagataac
    41581 aacaaacgga acaaagcgct ttccattgaa aaatgacaaa gcatccggca attttctata
    41641 attatagtac ttattataag ccattgcccc gacaaatcca gcgatgatcc ctacgaatac
    41701 tcccatattc aaagctggtg cttccaatac acttgtaaaa aatcctttaa ccatgatctt
    41761 cgtaccgaac agagtgtggg taaatgcatc ttcattcgca agcatgtcac ttgtgacacc
    41821 aaagatagcc ccagtgatcc gattgattaa aataaatgag atcccagctg caaatgctcc
    41881 gcctgctctt tcttttgccc aacttcctcc tattgctaaa gcaaataaaa tatgaagatt
    41941 accaataatt gcccaaccaa tattttcaat tactccgccc gttgtgacaa gcggcgtcca
    42001 atcaggattg atcaagggaa ttgatttccc tatactgatc atcaacccag ctgccggcat
    42061 aactgctacc acaaccatta aagcctttcc aaatttttgc caaaactcaa aactaaatat
    42121 ctttttcatt ccgtttagct cctttacgca atcggtttcg ttaaacgtat tatagcgtgc
    42181 gttttctttt ttgtaaatgc tttatttttt atttttctgt ctataccatt ctataaaacc
    42241 tataatacgt gtgttccctt ctataatttt ctcctaaaaa tttatttttt taagcttttt
    42301 tctatgaaat attttctttt gtctattgac ttgtgcaatc ggttgcgttt attatgaacg
    42361 tataaaacag ctcattagga ggagaaaaat gaaacagatc aaacgactat ttgctattga
    42421 tccatggaaa atcagcacga cacaattaga taaaaaaaat cttcgactgc aagaatcttt
    42481 aacaagtatt ggaaacggtt atatggggat gcgcgggaac tttgaagaaa cttactcagg
    42541 ggaccaccat caaggtactt atttggcggg tgtctggtat cccgataaga cacgtgtcgg
    42601 ctggtggaaa aatggttatc cagaatactt tggaaaagtg atcaacgcct tgaactttat
    42661 cgcgatagat atttcgatca acgatcagct gatcgacttg gcttctttgg aaccagaaga
    42721 cttttactgg gaattagata tgaaaaatgg tgtattatct cgtacattca caatcactac
    42781 ttctactaat aaagtacgca tttccttcga acgttttctg agcattgtca aaaaagaatc
    42841 agcttatatc tatttaacta tagaaatgct tgaaggtagc ggaacagtca aactcgtatc
    42901 aaaattggat ggaaatgttc aaaacgaaga cagcaactat gatgaacatt tttggaatga
    42961 gattgatcgt ggaactatag aagaaatcgg ctatttgacg actaaaacta ttccaaacaa
    43021 ctttgggatt gaacaattta ctgttactgc agcaatgcgc catttcgtta atggaaaatc
    43081 agtcatccct gactatcacg aagatacttt agcattacat ggttgcttgt ctgcagaatt
    43141 atctcaaggt gagacatggt cagtggaaaa agaaattatc gttcttacta gtagagatat
    43201 tccagaagcg cagcaagcag aaaaagctgt ctcagaatta acgaaccaga caaacaatta
    43261 tatccaagca aaagaagagc aaacagcggc ttgggcagaa cgatggaagc ttgccgatgt
    43321 ggtcatcgaa ggagacgaag aagcacaaca aggtattcga ttcaatttgt tccaactatt
    43381 ctctacttac tacggcgaag atgaacgatt gaatatcggc cccaaaggat tcacaggaga
    43441 aaaatacggc ggagccactt actgggatac agaagcgtat gctgtccctc tttatcttgc
    43501 tttagccaaa cctgaagtaa ctaagaatct gctgaaatat cggcataatc aactgccgca
    43561 agcaatccac aacgctcagc aacaaggatt aaaaggcgcg ctttatccta tggtcacctt
    43621 cactggtgtc gaatgccata acgagtggga aatcacattc gaggagattc accgaaatgg
    43681 cgcgattgcc tatgcaatct acaattatac gaattacacc ggcgatacgg aatatttgaa
    43741 aaatgaagga ctggaagttt tagcagaaat cgcacgcttc tgggcagacc gtgtccattt
    43801 ttcaaaacga gcaaataaat acatgatcca cggtgtgacc ggcccaaacg aatatgagaa
    43861 caatatcaat aacaattggt atacaaatac gatcgcagct tgggtcttgc gttacacgcg
    43921 agaaaattat ttaacttatc aaaaagaagc tgctatcact atcagcgaag aagaactagc
    43981 caaatggaaa gatatcgcag agaacatgta cttcccagtt gacgaagaat taggaatctt
    44041 tgtccagcat gacacattct tagataaaga tctcatgcct gtatccgata ttcccacaag
    44101 cgatcttcct ctgaatcagc attggtcttg ggacaaaatc cttagatctt gcttcatcaa
    44161 gcaggcagat gtactacagg ggatttattt cttcaatgat acttttacaa aagaagaaaa
    44221 gcaaaaaaac tttgatttct atgaaccaat gactgtccat gagtcttccc tttcgccaag
    44281 tatccattcg attttagcag cagaattagg aatggaaagc aaagctgtag aaatgtacca
    44341 gcgaacagct cgcttggatt tggataatta caataacgac acagaagacg gcttgcacat
    44401 cacttccatg actggtagct ggttgtcgat tgtccaagga tttgctcaaa tgaaaactgc
    44461 ccatgatcag ttgagattcg caccatttct gccaaaaact tggaaagctt attctttcca
    44521 tatcaattat cgcggacgtt tattgttcat ctcagtcaca gatgaacaag ttactttcga
    44581 gttattagaa ggttttcctc tagaaattta cgtctatgaa cagtcttatt ctttagcgga
    44641 tcgtctagaa ataacgctaa gaaaggagga agcaaatgtt taaaggggta ttgtttgatt
    44701 tagacggtgt cattaccgat actgctgaat tccattatcg tgcttggcga aagcttggtc
    44761 aggaaatcgg cattacgatc gaccacacat tcaacgaaca gctaaaaggc gtcagccgag
    44821 aagactcact ctctttgctt ttagcttatg gcggaaaaga acactctttt tcaaaggaag
    44881 aatttgcaga attagcgaaa agaaaaaacg attattattt agagatgatc cagacgatcg
    44941 aaccaaaaga tgtcttcccc ggggtcgtgc ctttactgga ttctttaaaa gaagaaaaaa
    45001 tcaagattgc tcttgcttct gcaagtaaaa acgggccatt cctacttgaa aaaatgggtc
    45061 ttacacctta ctttgatgct attgccgacc cggcagaagc aacaaacgga aaacctgcgc
    45121 cagatatctt cctccttgca gcaaaagctg tcggactaaa agcagaagaa tgtatcggga
    45181 tcgaagacgc gcaagccggt atccaagcca tcctctcaag tggcgctcaa ccaattggcg
    45241 tgggacgtgc tgaagacctt ggagaggaga tcccgcttgt ccctgatacg acttttctta
    45301 cgatcgatta cttgaaagaa aagtgtcatg accatggata atccagagat ctccgtcgta
    45361 ccagttccag agactgaaat aaagaaaatc acattgaaga acgactactt agaagtcgtt
    45421 cttctcaatt acggtgcccg cctgcaccaa atcttcgcac cagacaaaga aggaaaatca
    45481 gaaaatgttt tattgtctta cgatcgtttc gaagatgttc taacagacaa aagctttttt
    45541 ggagcaactg tcggacctgt agccggaagg atccgggatg cttcttgggg tgagcataga
    45601 ttggagaaaa actgcggatc acaccatatc cacggtggca cgaaaggctg gtctttccaa
    45661 ttttgggaag tagaagtttt caaaactcca tcatcaatag gtgtggtctt ttacttaaaa
    45721 gatgaatttt ctacctatcc aggaccaatc actgctacga tcacttaccg tttaacaaaa
    45781 aacgaattag agatgacaac ctctgctagt agccttgttg aaaccatctg caatccgacc
    45841 aaccatgcct acttcaacct ttcaggaaat ggaacaagag acatctatga acatcagtta
    45901 acggtctttt tagatggtgt gcttgaatta gatcaagaaa aactgcctac tggaaattgg
    45961 acaaaaaaag aaaaactgcc aatcgacttt agaaaatccc caacactgca agagatcctt
    46021 gcatgttatc cagatggact agatgatgta tttcttttgc atcatcctag gctttccaaa
    46081 acttccttac aattattcga gaagcattct ggtcgccaaa tgactatagc aacttctaat
    46141 aaaagtatgg tattattttc tacgactgga ttcgaagctg atttctctgt caatggaaaa
    46201 cagatgcatt caaactacgg attagcaatc gaaccgcaag aaatccctga tatcgttcat
    46261 ttccccaaat ttggatcgat caacctccat cctggtcaag aacggatcag tcagacgatt
    46321 tatcggttct ctgcacaata acaagagaaa ggtgttttta tcatggctat taccgtaaaa
    46381 gatgtggcga aaaaagccgg tgttgccacc tcgaccgtct ctcgtgtcat caacgatcat
    46441 cctagtattt ccgagacaac aaagaagaaa gtccgaaaaa ttatggatga actaggttat
    46501 gttcctaaca tgactgcccg caatctagga aaacgcattt ccagtgccat cggcgtaatc
    46561 ttaccccctc ttgattcaaa agagcggatc ggtaacccat tttatcttga gatcatggaa
    46621 gcaatcaacg aagaagcgcg gaattatgat atgactacag caatcgctac tgctaaaaat
    46681 tttgaattgc tgttagagaa cgtccagcgt atgcatttac aaaaacaagt agatggtttt
    46741 attctagtgt attcagatag tcaagatcct gtcattgatt atttatacca aaaccacatc
    46801 cctttcactt tgatcggaca gccttatcat catgagaacg aaatcgttta tgtagataat
    46861 gataaccagc ttctaggtaa acaagcaaca gaatttttga tccagaatgg acataagaat
    46921 atcctgtttg tgacaaacac gactcatgaa agtttgtatt tcgaacgata tttcggttat
    46981 caaaaagcaa tgatgatggc tggtcttcct gcgttatcca gtgtagtact agagcatccg
    47041 gaggattatc tgtcctttga gaaaacgttg aaagaaaacg aagccacagc attggtggtc
    47101 attgacgata tcttcgccct tcgcacgatg cagttggcgc aaatgtacgg ctatgtcatt
    47161 ccagagacac tctccgtcat tagcttcaac aactcgatct tctcaacatt aacacaccct
    47221 taccttacaa gtatcgatat cgatatctcc gaattaggac gtgtcgctat gcaaaagctg
    47281 cgcgaattga tccatgaaga attgagcagt ggcgtacgtt tggtcatccc tcatcgcttg
    47341 atcaaaagag agacagtcgt tcctttagaa aacccatact aaatagctga ctcactaaat
    47401 acgctgacca tttatcctat ggtcagcttt ttatgtaaaa gaagatacta cttctaacac
    47461 attgtttatc tgtaaaaa
//
LOCUS       DACEEZ010000013        46032 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20313_2017
            SAMN12639966-rid9879223.denovo.013, whole genome shotgun sequence.
ACCESSION   DACEEZ010000013 DACEEZ010000000
VERSION     DACEEZ010000013.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639966
            Sequence Read Archive: SRR10023158
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 46032)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 46032)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741683.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741683.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:44:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,016
            CDSs (total)                      :: 2,962
            Genes (coding)                    :: 2,889
            CDSs (with protein)               :: 2,889
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 36 of 73
            Pseudo Genes (incomplete)         :: 29 of 73
            Pseudo Genes (internal stop)      :: 18 of 73
            Pseudo Genes (multiple problems)  :: 8 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..46032
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639966-rid9879223.denovo.013"
                     /isolate="E20313_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(64..915)
                     /locus_tag="IWO89_000798"
     CDS             complement(64..915)
                     /locus_tag="IWO89_000798"
                     /EC_number="2.7.1.148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase"
                     /protein_id="HAQ0455571.1"
                     /translation="MELIEKAPAKINLGLDVLYRRQDGYHELEMVMSSVDLADHLFFE
                     EITEDKIIVETNKAFLPVDERNNVYQAALLLKNKYNIKTGVKIFVKKNIPVAAGLGGG
                     STDCAAALRGINQLWNLGCSLEELAEIGLEVGTDVPYCVYGSTAFVGGKGEEIQVLPS
                     MPQCWVVLVKPRMSVSTRTVFREVDTQAIQHQDIHGLAEAIREQDYQQMIKKMGNSLE
                     DITGKRHPVIHQIKERMIKYGADAALMSGSGPTVFALCQHYSRAQRVYNGLKGFCEEV
                     YLVRTLQ"
     gene            complement(1069..3642)
                     /gene="mprF"
                     /locus_tag="IWO89_000799"
     CDS             complement(1069..3642)
                     /gene="mprF"
                     /locus_tag="IWO89_000799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional lysylphosphatidylglycerol
                     flippase/synthetase MprF"
                     /protein_id="HAQ0455572.1"
                     /translation="MKNKRYLMLQWIKEHSLILKLIFFGSVLVFVANQVANIANGMSW
                     QDIWQRMGQQSRMTILLMILAGLLGVTPMLLYDWVTIRVLEKQGKPKMERKEFLLAAW
                     VTNTINNLAGFGGVVGASLRASFYGKQTNRKMVLATVSKVALFMLTGLSVWSFLTFID
                     VFFIQSESIFRSYWIWLFGGSFLAPVIFFFAYLKRKTLFFEFYPKGLLGLLLASFGQW
                     TGALAVFLIIGKLMRIDISMLSIYPMFMIATLIGMLTMVPGGMGTFDVLMILGMSQLS
                     VRQDVAVVWLLYYRLFYYVLPFMTGLLLFITHTGVKINRFLDNIPRLFFQKVAHIVLV
                     SAVYFAGIMMVLLSTITNLSAISRLFQVLLPFSFNFLDQTVNLLVGFLLLGLARALSV
                     KVKKAFLPTIALLSFGILNTITRTLSWQLILVYVLILLAVWLSRKEFYRTKFVYSWGA
                     IIFDASLFGFLFIVYAIAGYHSGRFLVGRITGNHFILFPSDDVWFSGLIGLGISLLAL
                     VALYQYLAEDTTKLGSEYEKDRFDRLLETYGGTATSHQLRLSGYSYFYYQEDDQDQVV
                     FGYQIKGNKCFVLGDPVGNPKMMRAATNQFMKQADCLGYQLAFYKISESYVVMLHELG
                     FHFTKVGEAGIVDLATEDLSRLSQQLEYQRLMKEGYHFTWYDQLPEELHQAVNEVSQK
                     WLQGAREKYFSVGRFDKSYVLSSGVGIVRKDQKVVGFITAQPMTKKQAGYDLLRVLPK
                     ESEELADYLLANLFIVYEKMGYSEANLGLAPLANVGETDFSFFQEKVMHIVYNYGNFF
                     YSFQSAYEKKLKYVDHWEGRYFAYRKGSNFGFATMQLLSLIGKEKGNVPSLAEEVLTE
                     L"
     gene            3783..4589
                     /gene="proC"
                     /locus_tag="IWO89_000800"
     CDS             3783..4589
                     /gene="proC"
                     /locus_tag="IWO89_000800"
                     /EC_number="1.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase"
                     /protein_id="HAQ0455573.1"
                     /translation="MKVGFIGTGNMAQAIIDGLLKKGTVSKEEVFVSSGHFENAQKFA
                     AETGVHACKSNQEVASQSDIVLLAVKPKIIEKVLEELREYSQKVLFISIASGKSLIDL
                     ETALEDPQASIVRVMPNVNVSVGAGVSAICKNEAATEEQLQQATMIFEAIGSVYPLAE
                     SDFSTFIGLAGSSPAFVYMLIDAMGRTGVLNGLSKQTATEIAAKAIMGSCQKFLSSDK
                     NPWELVDQVSSPGGTTVAGVVALEEAGFIPAIIKGINTTIEKDLEMLNKK"
     gene            complement(4668..6224)
                     /gene="rny"
                     /locus_tag="IWO89_000801"
     CDS             complement(4668..6224)
                     /gene="rny"
                     /locus_tag="IWO89_000801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Y"
                     /protein_id="HAQ0455574.1"
                     /translation="MVFNILLAIIGLIVGLGLGFMVAKSRHEKEIAGAQSSAAGIIDS
                     AKKEAETLKKEALLEAKEENQKYRSEVESELRESKLELKSQENRLIQREQTLNRKDDS
                     LEKRENSLESKEEALSSKQQLIEEREKDVEKMIEDQQKELEKIAGLSKEDARDVIMKS
                     TEEELNHELTLMVKESEQRAKEEADRKAKNLLSLAIQRCAADQVSETTVSVVTLPNDE
                     MKGRIIGREGRNIRTLETLTGIDLIIDDTPEAVVLSGFDPIRREIARMTLEKLIQDGR
                     IHPARIEEMVEKSRKEMDERIREYGEEAAFEVGAHTLHPDLIKIIGRLRFRTSYGQNV
                     LKHSVEVAKLAGILAAELGEDIQLAKRAGLLHDIGKALDHEIEGSHVEIGAELAAKYK
                     ENPVVINAIASHHGDVEATSVISVLVAAADALSAARPGARSESLENYIRRLRSLENIS
                     NSFAGVESSYAVQAGREVRVMVKPEEISDLDAVRLVRDIRKRIEEELDYPGHIKVIVI
                     RETRATDYAK"
     gene            complement(6635..7684)
                     /gene="recA"
                     /locus_tag="IWO89_000802"
     CDS             complement(6635..7684)
                     /gene="recA"
                     /locus_tag="IWO89_000802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HAQ0455575.1"
                     /translation="MADDRKAALDAALKKIEKSYGKGSIMKLGEKIDQQISTIPSGSL
                     ALDVALGVGGYPRGRIIEVYGPESSGKTTVALHAIAEVQKNGGTAAFIDAEHALDPQY
                     AQKLGVNIDELLLSQPDTGEQGLEIADALVSSGAVDIVVVDSVAALVPRAEIDGEMGD
                     SHVGLQARLMSQALRKLSGSINKTKTIAIFINQIREKVGVMFGNPEITPGGRALKFYA
                     TIRLEVRRAEQLKQGTDIVGNRTKIKVVKNKVAPPFKIAEVDVMYGLGISQEGELLDM
                     AVEKDIVDKSGAWYSYKEDRIGQGRENAKIYMANHPEMMAEVSALVRAAYGIGEEVAV
                     PEDEKGQEELPLVEE"
     gene            complement(7793..9034)
                     /locus_tag="IWO89_000803"
     CDS             complement(7793..9034)
                     /locus_tag="IWO89_000803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence/damage-inducible protein A"
                     /protein_id="HAQ0455576.1"
                     /translation="MKAEIIAVGTELLLGQVVNTNATFLSEQLADLGIEVYYQTVVGD
                     NQQRLEELIALAETRSELILLCGGLGPTEDDLTKEATAAHLGKSLIQNTEGYKKLLAY
                     FETTHRKMTKNNLQQSQIIEGGVPLPNRTGLALGTFYQTDTHAYILLPGPPNELKPMF
                     VEQVRPLLEERFPSEEKLISKVLRFYGIGESRLVTELKDLIETQINPTIAPYAKPNEV
                     TLRLTVKTNDVQAGNQALLALEEKIQERVGEYFYGYGDDNSLAKVVVELLKENKQTVT
                     AAESLTAGAFQAALGDIAGVSEVFPGGFVTYSLQTKAGFLEIDPELLAEYGTVSKECV
                     EQMAIQAREIARTDYSVAFTGVAGPDTLEGQSAGTVWIAISSKDGVVSEKYHFARDRA
                     AIRYNAVMQGFDLLRKQLLRK"
     gene            complement(9274..9852)
                     /gene="pgsA"
                     /locus_tag="IWO89_000804"
     CDS             complement(9274..9852)
                     /gene="pgsA"
                     /locus_tag="IWO89_000804"
                     /EC_number="2.7.8.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010754872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--glycerol-3-phosphate
                     3-phosphatidyltransferase"
                     /protein_id="HAQ0455577.1"
                     /translation="MNLPNKLTVLRICMIPIFILVVGLPLDWGQFNVGDTTLAVTQLV
                     GAVIFALASITDWLDGKIARARGLVTNFGKFADPLADKMLVMTAFIMLVDQGKAPGWV
                     VAIIVCRELAVTGLRLLLVEHGEVMAAAWPGKVKTATQMVAIILLFINNIPFSLIGIP
                     VDQIMLYACLIFTIYSGVDYFIKNSGVFKGSM"
     gene            complement(10061..10945)
                     /locus_tag="IWO89_000805"
     CDS             complement(10061..10945)
                     /locus_tag="IWO89_000805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAQ0455578.1"
                     /translation="MAFETIGEKLRQARLNKKISLDELQQITKIQKRYLEAIDNDDFD
                     QLPGKFYVRAFIRQYAEAVGEDGDHLVDVFDGKSKLTGSVPERPQPETVSTSRKAMHQ
                     EESHPSRFWTSLPVILLGLVALAIVVVVGYMTWQDRQADPIIGDTASSLKVEGSVEES
                     SSSEQSTQTKESSTVESTTKSSEEKKMAIATGEDSGSAIAVNITDATKPVTLEFTAVN
                     RVWLGVLVDNAYVYQGTLAANETQSTVLPETATNATITIGAASNATIKANGESVPVNP
                     GENNQSPKNVNLTLQYAE"
     gene            complement(11723..13009)
                     /locus_tag="IWO89_000806"
     CDS             complement(11723..13009)
                     /locus_tag="IWO89_000806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HAQ0455579.1"
                     /translation="MNKTEYEQINETLYHEVLPNGLTVYLLPKNDYHKTYGLFSTNYG
                     SIDNEFIPYGEKEKVKVPDGIAHFLEHKLFEKEDGDVFQLFGKQGASANAFTSFTKTS
                     YLFSTTDQVEKNLTTLIDFVQAPYFTEETVNKEKGIIGQEIQMYEDDPNWRMFFGILN
                     NLYPTHPLHIDIAGTVESIDKITAQDLYTCYRTFYQPSNMVLFVVGKMEPEKLMKLIR
                     ENQEAKNFPPKQEIVRYFPENTKEIIKQSALEAAITRDKFVLGIKGLDTLPQEGTELL
                     RYKTAINLLFQMILGNTSRNYLAMYNQGIIDDSFGFEFSLDREFHFADFSGDTDEPEK
                     AAEKVKEIILGFADDPEVSETNLDLLKKKMLGQYFQSLNSIEYIANQFTQSLFGDRTL
                     FDLPEIIDSIQMKDVLAAGEAFISEEAFSEFYMRPK"
     gene            complement(13002..14270)
                     /locus_tag="IWO89_000807"
     CDS             complement(13002..14270)
                     /locus_tag="IWO89_000807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HAQ0455580.1"
                     /translation="MVYQLAEGVNLHVLPTKQYKTIRIFIRFTARLQQEVITKRSLLS
                     SMLETNSLNYSDQTKLSAQLAELYGASFGLSVRKKGNLHWLNAGISFVNGEYVNDPNL
                     FSQAVDFLKEVLFYPNIKNHQFDQLTFDLEKNNLRLYLESLKEDKQTFASYALQELYF
                     ENSPEQKIPGLGVVEDLDKITASSLASYYQEMMANDQIDIFVVGDVDPDKAAEAVGQL
                     PFESRETVYPDLFYTQPQVNIVKERQVREPIVQAKLNLAYQTNVYYDEPERFALMVFN
                     GLFGGFPHSKLFMNVREKESLAYYASSSVDTFRGFMSVQTGIDEKNRNQVLHLIHEQL
                     ESLRNGEITELELSQTKAMLRNQYLLSLDSPQAAIEASFLDSWLPETKLSDEEWLKRM
                     ESVTIKEIQQVAEQIELQAIFFLAGGNANE"
     gene            14522..14983
                     /gene="mscL"
                     /locus_tag="IWO89_000808"
     CDS             14522..14983
                     /gene="mscL"
                     /locus_tag="IWO89_000808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="large conductance mechanosensitive channel
                     protein MscL"
                     /protein_id="HAQ0455581.1"
                     /translation="MIKEFKEFIMRGDVLDLAVGVVIGSAFTSIVNQVVQGLITPLIG
                     WFVAMITGTSDLEGALSILDWSPTKGVTFAFGNVISAIITFLITGFVLFLVVKAANRA
                     KNLRSKEEQVEVEETPTAEDYLSDIRDLLAKQVAEDNTHPVEQTNLSDTSK"
     gene            complement(15043..15489)
                     /locus_tag="IWO89_000809"
     CDS             complement(15043..15489)
                     /locus_tag="IWO89_000809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAQ0455582.1"
                     /translation="MEYQVKKFEELSTAEFYAIVKERIAVFVVEQQCPYQEVDDIDGE
                     AWHTFFQDTDGKILAYTRVYEEAESIHFGRVLVHQDNRKDGLGRKIVQETINMIQKNF
                     PEKPIVIGAQEYLQAFYESFGFKAISDVYLEDDIPHIDMKKEQVAS"
     gene            complement(15582..15827)
                     /locus_tag="IWO89_000810"
     CDS             complement(15582..15827)
                     /locus_tag="IWO89_000810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0455583.1"
                     /translation="MPTTLASIKKDLEGRIGSPIMLVAQTGRKRQTERRGVLTETYPS
                     VFVVDLDPEENSFERVSYSYSDVLTRTVEIEFLTEAV"
     gene            complement(15943..16521)
                     /locus_tag="IWO89_000811"
     CDS             complement(15943..16521)
                     /locus_tag="IWO89_000811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HAQ0455584.1"
                     /translation="MSEELLRAARNGDEEAFAALYQKYFPLLLRLKNTYYLRNYVSED
                     WEQEARIALYQALRTYEKSKGVSFGSYYKSVISNQIYSLLRKQNALKRRDQKDEVSID
                     QKIETEGPDFLAEITLQEPLAIQHLLLKEAIENCEIQFSKIEAKIFHYYMQGVDFVDM
                     AECLAMDHKQVMSAYGRVKLKIKKHLKFYNEE"
     gene            complement(16598..17128)
                     /locus_tag="IWO89_000812"
     CDS             complement(16598..17128)
                     /locus_tag="IWO89_000812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NYN domain-containing protein"
                     /protein_id="HAQ0455585.1"
                     /translation="MKKQLLIVDGYNMIGSWPELVQLKKQDKMADAREALLHRLSNYA
                     KYEGMEVIVVFDAQLVPGIQQNYKKYQLDVVFTKEDETADSYIERIAGQLNDRLTQVT
                     VATSDLAEQWMIFSQGALRTSAYELYKTIKKTEKTIAQENKDMHFIDFRRNSPWNEEQ
                     LKKLSQKLDDLMRKNK"
     gene            complement(17132..17992)
                     /gene="rlmB"
                     /locus_tag="IWO89_000813"
     CDS             complement(17132..17992)
                     /gene="rlmB"
                     /locus_tag="IWO89_000813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (guanosine(2251)-2'-O)-methyltransferase RlmB"
                     /protein_id="HAQ0455586.1"
                     /translation="MGKERTNKHQSKRGFEDKKQNRYSNKKQTGRNQSVSDQNEEALE
                     DNFVFGHHAAVEALQQGRGNKLFLQEDSRGEKVEELKEIAREQAVPVKWVPKQKLETL
                     SDHGVHQGVVLAITPYEYLSLDQLIERTKEKKEDPFFLILDSIEDPHNFGSILRTADA
                     SGVDGVIIPKHRAVGITPIVTKTSTGAVEHVPVARVTNLVQAVQQLKETGFWVFGTDM
                     TGTNFQKWNAKGSIALVIGNEGKGMSPGLKKEMDELLTIPMTGHVQSLNASVAAGLLM
                     YQAFSQRQGE"
     gene            complement(17985..18380)
                     /locus_tag="IWO89_000814"
     CDS             complement(17985..18380)
                     /locus_tag="IWO89_000814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HAQ0455587.1"
                     /translation="MRDYTQLNGLALAYVGDAIYEIYVRDHLIDQGETKPNKLHRAAT
                     NYVSANAQAFLIQQMLEEHLLTEEEELYYRRGRNAKSHTSAKNADVTTYRVATGFEAL
                     MGYLHLTKQTERMEELIRWCIQKVGEKNG"
     gene            complement(18377..19789)
                     /locus_tag="IWO89_000815"
     CDS             complement(18377..19789)
                     /locus_tag="IWO89_000815"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HAQ0455588.1"
                     /translation="MIKIYNTLTRKKEEFIPIQKGHVGMYVCGPTVYNYIHIGNARST
                     IAFDTIRRYFEYRGYKVDFVSNFTDVDDKIIRTANELGITAPEVADRFIRAFEEDTKA
                     LNVEPATLHPRVMDHMPDILAFIQVLVEKGYAYESSGDVYYRTRKFENYGKLSDQSID
                     ELEVGASQRTGEEQQQKEDPLDFALWKQAKPGEISWDSPWGKGRPGWHIECSVMATKH
                     LGETIDIHGGGQDLEFPHHENEIAQSEAKTGKKFANYWMHNGYVTIGEDDEKMSKSLG
                     NFITVHEMIKNVDPQVLRFFMATTQYRRPIRYSETTMKEAATNLQRLKNTFDNLKFRQ
                     ETASENLADDADKIALLENLEQRFIEEMDDDFNAANGITVVYELAKWLNTYLDQPKVS
                     AAVLAQGEALFSQWLSIFGILFTAEELLDEEIEQLIEERNQARSNRDFVRSDEIRDLL
                     KEKGILLEDTPQGTRWRREA"
     gene            complement(19786..20322)
                     /gene="cysE"
                     /locus_tag="IWO89_000816"
     CDS             complement(19786..20322)
                     /gene="cysE"
                     /locus_tag="IWO89_000816"
                     /EC_number="2.3.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine O-acetyltransferase"
                     /protein_id="HAQ0455589.1"
                     /translation="MSWFTRAIDAAKRNDPAARSTAEIVLTYPGFHALFWHRFSHFLY
                     QHRLFLLAKINAQFWRFLTGVEIHPGATIGTGVFIDHGMGIVIGETAEIEDDVILFHG
                     VTLGGTGKETGKRHPTVKQGAMLSANAQILGPVTIGKNAKIGAGAVVLKDVPDDATAV
                     GVPAKVVRIKGEKVRRSE"
     gene            complement(20644..22104)
                     /locus_tag="IWO89_000817"
     CDS             complement(20644..22104)
                     /locus_tag="IWO89_000817"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HAQ0455590.1"
                     /translation="MTKVRVRYAPSPTGHLHIGNARTALFNYLFARHNDGDFIIRIED
                     TDQKRNIEDGEKSQLENLAWLGMNWDESPENPGEYGPYRQSERKEIYQPLIDQLLASN
                     RAYKCYCTEEELEAEREAQRARGEMPHYAGTCANLTPEEQAEKEAQGLESVVRFRVPR
                     NTEYAFTDMVKGAISFESDNIGGDFVIQKRDGMPTYNFAVAVDDHLMKITHVLRGDDH
                     IANTPKQLMIYEAFGWTPPTFGHMTLIINSETGKKLSKRDESILQFIEQYRELGYLPD
                     AMFNFIALLGWSPVGEDEIFEHQELIKMFDPKRLSKSPAAFDAKKLQWVNNHYIKAMD
                     LDAFTDMCLPYLIADGRVAENPDEKTIEWVKKIVSLYQPQMSYAAEIVDLSTLFFNEH
                     PVLNEAAKEVLAGETVPTVLHAFKAQLEQMEVVDAPTVKAAIKAVQKETGVKGKNLFM
                     PIRVAVSGQMHGPELPETIELLGREKSLAHLNQVLN"
     gene            complement(22274..23431)
                     /locus_tag="IWO89_000818"
     CDS             complement(22274..23431)
                     /locus_tag="IWO89_000818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN/TRAM domain-containing protein"
                     /protein_id="HAQ0455591.1"
                     /translation="MQKRVITLLMIIVGASLGISFLPSAWQAIGQQSNGWLNNTFTNS
                     LLGALIFFLLSLLLAKHISAIIKQVEQKLSEVSLTYLLFGAIGAIIGLTLGVVISTPL
                     YNMEIPFVNSVLPILLMIILGYLGLRMGTTRIEEWKKVFGSRSKKIEQETASQVESQL
                     LERKSGENFHKYKILDTSVIIDGRIYDITKTGFLEGTLMIPNFVLYELQYIADSGDSL
                     KRVRGRRGLDILNALQKEEGISVEMYDGDFEDISEVDSKLIKLAKLLDGVIVTNDYNL
                     NKVSEFQNVPVLNINALANAVKPVVIPGETMNVLVVKAGTERQQGVAYLDDGTMVVVE
                     DGQHYMNERIEVVVTSALQTAAGRMIFAKPAHAGRGIDNKKETEKKTKQSK"
     gene            complement(23465..24835)
                     /gene="radA"
                     /locus_tag="IWO89_000819"
     CDS             complement(23465..24835)
                     /gene="radA"
                     /locus_tag="IWO89_000819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadA"
                     /protein_id="HAQ0455592.1"
                     /translation="MAKKSKVQFVCQNCGYVSPKFLGRCPNCGKWNTMVEEIEQDTTD
                     RRTRTSLTGEKAKPTKIADVVPKKEPRIKTKLEELNRVLGGGVVPGSMVLIGGDPGIG
                     KSTLLLQVSQQLAAIGGKVLYVSGEESAEQIKLRAERLGSINTEFYLYAETDMNEISR
                     AIEHISPDYVIIDSIQTMTQPDITSVAGSVSQVRETTAELLKTAKTNGIAIFIVGHVT
                     KEGSIAGPRMLEHMVDTVLYFEGEQHHSFRILRAVKNRFGSTNEIGIFEMHEHGLEEV
                     ANPSQIFLEERLDGATGSAIVVAMEGTRPILVEIQALVTPTMFGNAKRTTTGLDFNRV
                     SLIMAVLEKRAGLLLQNQDAYLKAAGGVKLNEPAIDLAIAVSIASSYKEKGTQPTECF
                     IGEIGLTGEIRRVSHIEQRVKEVQKLGFTKVYLPKNNLGNWEAPKGIEIVGVATLAET
                     LKRVFR"
     gene            complement(24898..25425)
                     /locus_tag="IWO89_000820"
     CDS             complement(24898..25425)
                     /locus_tag="IWO89_000820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dUTP diphosphatase"
                     /protein_id="HAQ0455593.1"
                     /translation="MRKRGFEVISKYKDQNINLPQRATHHAAGYDFEAAEEVVIPSIW
                     KAGWKKGLETVADGLKKDVSENEKQLKPTLVPTGIKAYMGEDEFLQLANRSSNPLKRF
                     LVLTNGVGVIDSDYYNNEANEGHIMFQFTNFGFKDVVIKKGERIGQGIFLPFLKADQD
                     QTTAQRQGGFGSSSK"
     gene            25655..26461
                     /gene="proB"
                     /locus_tag="IWO89_000821"
     CDS             25655..26461
                     /gene="proB"
                     /locus_tag="IWO89_000821"
                     /EC_number="2.7.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate 5-kinase"
                     /protein_id="HAQ0455594.1"
                     /translation="MRKEIKNAKRIIVKVGTSTLIYPNGNINLAAIDQLAFVLSDLRN
                     RGKEVILVSSGAVGVGMHKLHLSERPTLIPEQQAAAAVGQAELMNIYNQRFSVYNQQT
                     AQILVTRDVVDYPESRKNVRNTLEQLLIMDTIPIVNENDTVAVDELDHLTKFGDNDQL
                     SAIMAKLVAADLLIVLSDIDGFYSDNPTTNPEAQMYRYIDKIDEKILAQAGGAGSTFG
                     TGGMQSKLKAASRIFEANSAMILANGKNPAIIFDILAGKEVGTLFKEDLK"
     gene            26458..27705
                     /locus_tag="IWO89_000822"
     CDS             26458..27705
                     /locus_tag="IWO89_000822"
                     /EC_number="1.2.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-5-semialdehyde dehydrogenase"
                     /protein_id="HAQ0455595.1"
                     /translation="MTDLMQLGQQAKDSAQQLALMPTNRKNELLVQMAQTIKKNQQAI
                     IEANHKDLEKAVENNISETMQDRLQLTPERINAMATEIEKIASLDDPIGKVDEMWTNT
                     DGLRIGKKRVPLGVIGIIYESRPNVTTDAASLAFKSGNAVILRGGKEAFFSNQLLVQL
                     LQQVLVSAGESPYAIQFVDDTSHETAGQLMRLTEYLDVLIPRGGAKLIQRVKEQATVP
                     IIETGTGNNHVYIDKEAQLSMAVNIIVNAKASRPSVCNAAETLLIHSEIAPFFLPAIE
                     KELVEHGVSLRADARALEYLETAALAEDTDWDTEYLDYILAVKVVDSLDEAIAHINRH
                     NTKHSETIVTDSYAASQRFLNEVDAAAVYVNASTRFTDGSVFGFGAEIGISTQKLHAR
                     GPMGLNELTSTKYIIYGDGQIRE"
     gene            27781..28125
                     /locus_tag="IWO89_000823"
     CDS             27781..28125
                     /locus_tag="IWO89_000823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0455596.1"
                     /translation="MFEILMIFFIYLISLNIAAFLGVGILSLFFQFKKRSIGSQREKW
                     SQYFDKIGQKGLVTRLHISYMVALCLLATINYYSFFDHSIAYTITLLIAGIFHLSYKY
                     QLNKNHLNRTFR"
     gene            complement(28242..28568)
                     /locus_tag="IWO89_000824"
     CDS             complement(28242..28568)
                     /locus_tag="IWO89_000824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008266141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0455597.1"
                     /translation="MLEEYFQEVGLTLVELPIEFSDDRLIYRGYIKELPIINAEGETR
                     KAMQQKIAGMYQVYREQLLAESAEEEKEKTMNLSMDELLRYYDGETFDGFSLLNEEET
                     DLPGSW"
     gene            complement(29183..30385)
                     /locus_tag="IWO89_000825"
     CDS             complement(29183..30385)
                     /locus_tag="IWO89_000825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAQ0455598.1"
                     /translation="MEILENQIDIQEQTNEETKGKSSALVVAATCMIAFMGVGLVDPI
                     LKTIAAQLNATPAETTLLFTSYMLVTGVVMLFTGFLSSRIGLKKTLMTGLFIIVVFAG
                     LGGLSGNIGMLIGLRAGWGVGNALFVSTALATIVSILVGNTEKAIMMYEAALGCGMAV
                     GPLVGGVLGSGSWRYPFFGVAFLMFCGFIALAVLLPETEKPKRKVGLWEGVKALSNHK
                     LRSVGLIALLYNFGFFTLLAYAPFLLIGYSELEVGFVFFGWGVLLAIASIFVAPALER
                     KFTTYRTILAAFVLFIICLLLLGAGTSMPVLVPISIVLAGFFQGIINTLLTTIAMEIP
                     GLQRNVASSSYSFVRFFGGALAPFIAGVIGEHIGESYAFYFAGAIVLISVCCIIYHRN
                     YFATNEEE"
     gene            30502..31017
                     /locus_tag="IWO89_000826"
     CDS             30502..31017
                     /locus_tag="IWO89_000826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HAQ0455599.1"
                     /translation="MNTLAYILLCVLLSKPRSGYELKQLITIFWEAHHSQIYTTLAKL
                     DQQGYVTVLDKDEHSQKKIYHLTDEGRALVEEWIKEETPAPTQKDEFLAKIYAFSTLD
                     KTTATGLLFTREQQLNKIMQKNQKKLNDLSLDKKQEFGRYVVIQRRIMLCQQELQWCQ
                     QVREQMQQFFE"
     gene            complement(31216..31662)
                     /locus_tag="IWO89_000827"
     CDS             complement(31216..31662)
                     /locus_tag="IWO89_000827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2798 domain-containing protein"
                     /protein_id="HAQ0455600.1"
                     /translation="MPTNKKEGIIFTTMMCFLMVLGMSAYNLWLHQDLHLSSLLIGLV
                     PGFVVAFILDVFIIGVLAKKIAFKLPVNPESKVQMILTISCLMVLGMVTCMSLFGVIM
                     EQGIPDQLFSAYMNAWKMNVIAALPLQLIIVGPLSRSVLKAIQSNE"
     gene            complement(31709..33187)
                     /gene="msr"
                     /locus_tag="IWO89_000828"
     CDS             complement(31709..33187)
                     /gene="msr"
                     /locus_tag="IWO89_000828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Msr family ABC-F type ribosomal protection
                     protein"
                     /protein_id="HAQ0455601.1"
                     /translation="MENLAVNITNLQVSFGNQLELSIDSLRVYQQDRIGIIGENGVGK
                     STLLKLIAGELFPDHGKIQTEITFNYLPQLTYLAEAKDLNLELASHFQLRLEETSERK
                     WSGGEERKIELIRLLSSYEQGMLLDEPTTHLDRKSIDRLIEELRYYYGTLVFVSHDRY
                     FLDELASKIWEVKDGEIREFSGNYSAYLTQKELEKKTQLREAESIMKEKKRLEKSIQE
                     KKKQAEKLEKVSSKKKKQQIRLDRLSSSKQKDSVQKAIQKNAKTLERRLQKIGETTKP
                     QQMKQIRFPVPKSLELHSRYPIMGQNVQLERSGRTLLVNGDFQFSLGKKIAIVGENGS
                     GKTTLLEHIRKQGEGILLSPKVSFQVYQQKGYQMTSEESIIRFVMRQTEFSESLVRSL
                     LNHLGFAQETLTKPLCTLSGGEATRLTIALLFTKPSNVLLLDEPTNFIDMATIEALEK
                     LMQIYPGTILFTSHDSYFVERTADEVYEIKGQKIKKVLTRNF"
     gene            complement(33541..34170)
                     /locus_tag="IWO89_000829"
     CDS             complement(33541..34170)
                     /locus_tag="IWO89_000829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HAQ0455602.1"
                     /translation="MEQKNDFSTIMTGRRSVRVYDETYKIPHEEMLEMIKEATTAPSS
                     VNMKPWRFVVAESEEAKEILRPLIRFNTRQNDTSSAMVMIFGDMQCYEYGEEIYNQAY
                     ESGKMPKEVRDQQLAAIIPYYKSFSREEMNDVVKVDSSLAAMQFMLVARDHGYETNPI
                     GGFEADQLAEAFGLDKDRYVPVIILSIGKAVEEGYESVRLAPEKITMFE"
     gene            complement(34154..34618)
                     /locus_tag="IWO89_000830"
     CDS             complement(34154..34618)
                     /locus_tag="IWO89_000830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HAQ0455603.1"
                     /translation="MSLEEISHLLYQIKLADQKIVQIFEENIGISLTRYEIMMRLKEE
                     NPLLQSSLQEKLQIDQGAVTRHLKVLEANGYVSRQRNPQNNREVYVALTEKAQKKIKH
                     CEMDHKDTGSVLSPAFSDVEMKQFLTLLDKLNTQLCNITTETKEVNNNGTKK"
     gene            complement(35105..36373)
                     /locus_tag="IWO89_000831"
     CDS             complement(35105..36373)
                     /locus_tag="IWO89_000831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannosyl-glycoprotein
                     endo-beta-N-acetylglucosaminidase"
                     /protein_id="HAQ0455604.1"
                     /translation="MKAKNLLFSVILLGNLAVANTTVLAEESTETVATQTSSSSETGI
                     ENDTESTLQSATVSENTISTEEPTTETTEQTQTEDSTSAEAQNNPENAEEITNQLTDG
                     QTLTHTHQADLSEFENYTGYAFYARSSANSQQAFIDSIASTAQSLASANDLYASVMIA
                     QAIVESGWGNSTLASAPNYNLFGIKGSYNGQSVTMPTSEYVNGQWITVNAAFRKYPSY
                     KESLQDNVTVLKTTSFQPGVYYYSGAWKSNTNSYKDATAWLTGRYATAPNYGSTLNNV
                     IETYNLTQYDTAPTSTSPMYRLYNRHTGEHLYTLNAGEKNYLPTVGWEYEGIAWQAPN
                     SGQPVYRLYNPYSGDHHYTMAQSEINSLTKIGWRYEGLSFYSGGSKPIYRLFNPNEKT
                     GTHHYTLSAKERDWLTPMGWRYEGIGFYGY"
     gene            complement(36604..37341)
                     /locus_tag="IWO89_000832"
     CDS             complement(36604..37341)
                     /locus_tag="IWO89_000832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HAQ0455605.1"
                     /translation="MNDLSEKVIVIMGASSGIGEATTKLLAEKGAKLVIAARREDRLK
                     AIKESLPEAELYIQTADVRDFAQVQAVIDLAMEKFGRIDVLYNNAGIMPTAPLVEGHR
                     DEWQNMLDINIMGVLNGISAVLPIMEKQKSGHIISTDSVAGHVVYPDSAVYCGTKFAV
                     RAIMEGLRQEQRQNNIKSTIISPGAVQTELYQTISDKKAALELHEAQKEWGLTSEDIA
                     SAVAFAIETPDRMSVSNMIIRPTTQEV"
     gene            complement(37408..37977)
                     /locus_tag="IWO89_000833"
     CDS             complement(37408..37977)
                     /locus_tag="IWO89_000833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar O-acetyltransferase"
                     /protein_id="HAQ0455606.1"
                     /translation="MTSLIEKQIVNKEIRVTDPLFEVIHQIQAENEEPLAALNTGYHE
                     PADIRKRLENIISDKVDDTVTVLLPFYTDFGKHISIGKNVFINRQVMFVDLGGICLED
                     SVLIGPRVNLITVNHLTDPKERRGLSVKPIHIKKNAWIGAGATILPGVTIGENAIVAA
                     NATVTKDVPDNTIVAGIPAKIVKPVERII"
     gene            38105..38977
                     /locus_tag="IWO89_000834"
     CDS             38105..38977
                     /locus_tag="IWO89_000834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HAQ0455607.1"
                     /translation="MDIRILTYFLAVAREKNISRAAKSLHITQPTLSKQLKDLEEELG
                     VTLFTRGSREIQLTDSGRYLYSKGKEILSLVEKTANNLTNDAIISGEIYIGGGETKAI
                     SFISDSLHSLIKAHPDIQFHLYSGNADDIIDKLDSGLLDFGLVIEPTNKQKYQSFRLP
                     AQDRWGLLIHRNHPLAKQTVIHPQELKTVPLIISRQTSVDSQLAEWLGFSIDQLSIVG
                     TYNLLYNASLMVDNGTFGALALDGIINTYGTEKVFIPLDPPLQASLNLIWKKDQPLSP
                     AARKFLDVLTEKYS"
     gene            39319..40191
                     /locus_tag="IWO89_000835"
     CDS             39319..40191
                     /locus_tag="IWO89_000835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAQ0455608.1"
                     /translation="MKIKKIFNQNAVLVDDGGQEKVAIGKGIGFDRKRNDLIFDRDVE
                     RIFVMEPEGQIKLQNLLNQIDERFLFAAETIIDHAETVLMEKLNEHVLIALTDHIAFS
                     AENINNGIVIRNKLLREIEVLYSDEFSIAQWAVEYLTKELGIPYTYDEAGYIAIHIHS
                     GRSGRNNNHRSIREVTIVSDIIHLVESELETDIHSEPFSLNYSRLVNHLRLLLQRTHA
                     QQYAVLDTEIVEMVKRKYPESYKISKKIRVLLTKEYQLAITKEELGYLAIHIERLRSA
                     IVQTNVNNHEEEKN"
     gene            40188..42146
                     /locus_tag="IWO89_000836"
     CDS             40188..42146
                     /locus_tag="IWO89_000836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAQ0455609.1"
                     /translation="MKAYLQRMGRSLQLPVAVLPAAALLVGIGNWWASYSNDIVAHFL
                     QAGGNAVLGQLPLLFAVGLALGMSVDKSGAAALAGLVAFEVPVNVLKTDSVATLLNIK
                     PESVDPSFAQISNVFIGIIAGLIAAALYNRFHEAKLPMALSFFSGKRLVPILAAFVML
                     LISAVLLLVWPPVYSALVSFAKFILSLGWGGAGLYGFFNRLLIPTGLHQAMNSVFWFD
                     VAGINDIGKFLASEGTKGVTGMYQAGFFPIMMFGLPAGAYAIYRNARPENKAKTASLM
                     LAAAFASFFTGVTEPLEFSFMFVAWPLYVLHAIFTGISLAVSAFFHWTAGFAFSAGFV
                     DYFLSLKNPIANQPLMLIVQGLVTAVIYYFGFNFAIKKFHLMTPGREEADLSDDDTAT
                     TNSDNKYAAQASKIYAALGGADNVTSIDNCTTRLRLQVKDTGLIDQNKIKATGVPGMK
                     IIDGKNAQVIVGTEVQFVADEMAKLHGGAATRPAQTNTVVKTETFAGGTIEQDIYAIA
                     NGKLIPITEVSDDVFAEKMMGDGYAVLPENGEIFSPIAGTITNIFPTKHAVGIQTDAG
                     IEVLLHMGINTVDLKGEPFTLYVKEGQKVARGQLIALVDLAAIQSAGKNTDMVVVFTN
                     GDKVQSLEIEPARDVKANDKIGSVSNKA"
     gene            complement(42478..43329)
                     /locus_tag="IWO89_000837"
     CDS             complement(42478..43329)
                     /locus_tag="IWO89_000837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HAQ0455610.1"
                     /translation="MHYLVTGATGGFGGYALEFLKKMVPQNEIYVLVRSEEKGEALKE
                     AGFNIRIGDYDDLDSMKEALKGIDRLLFVSGVPGNRQAEHKNVVKAAQAAGVSYIAYT
                     SFADADHSISVLAPDHQFTEKMIKDSGISHTFLRNNWYVENELPIIGQALKTGQFVYA
                     AGNGKTGWALKREYAEAAAKVLVESDSPEILELSGNLTDYEELAKALERATGKKLEII
                     EASDAAFVENLKEAGFPQEAADMFLAIQHDIKKDQLAISSDDLEKVLGKPLTSLEAAL
                     KEILDQE"
     gene            43452..43919
                     /locus_tag="IWO89_000838"
     CDS             43452..43919
                     /locus_tag="IWO89_000838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HAQ0455611.1"
                     /translation="MKYSLQFSDAIHVLAYIEMFQDTRSLSSEMIASSIETNAANVRK
                     IMSQLKKSGLIFTKTGKPQPSLAKSPDQISLLDVYRSIEGNTQLIQVDPKTNPNCVVG
                     ANIQEALEESYARLQKKAEEEMAKITLDSLIHKIAVLEKEKRPENTGLVERFL"
     gene            complement(44182..44664)
                     /locus_tag="IWO89_000839"
     CDS             complement(44182..44664)
                     /locus_tag="IWO89_000839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HAQ0455612.1"
                     /translation="MAKYRIEEKPDFTVTGIGVELKSSNTDFVGLAAEKQAFEQKVIG
                     NGTIDALKQAAENHYFFTVNEPYNGKMMYYVGVQTSQDVPNTTRIIPFPKGTYLVVIA
                     LAESPEELRNDLTNTAFGHVLREVEDYAYVGGPNAVVEMGVTNGKYMGEIWIPVVNKP
                     "
     gene            complement(44764..45627)
                     /locus_tag="IWO89_000840"
     CDS             complement(44764..45627)
                     /locus_tag="IWO89_000840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HAQ0455613.1"
                     /translation="MLKDLNQLIDYIEEHLTEGLSYKIIAENTGISEYQLKRIFTFIA
                     GMSLMDYIKNRRLALANRDLLDGSRVTETAFRYGYETVEGFSRAFRDWSGYLPSEISR
                     YGIQKSFPRLTFFIDVRGGKSMEFRIEKKEQFYVAGVAKKVPVKFEGVNQEIQKLAQT
                     ITETQREEMHKLGDLYPNQVINVSYGFDGARLEEKGSLMHMIGFATTKENPYADLEML
                     AIEEHTWAIFPNKGPFPQTLQDTWGKIYAEWLPSSDYELVEAPEISFTKWGEDFSNVY
                     SEIWIAVKAKK"
BASE COUNT        13129 a         9840 c         8239 g        14824 t
ORIGIN      
        1 aaaactgaag aataccattt taggattcct cagtttttca tcacctattt tacttcttat
       61 attttattgc agggttcgta ctaaataaac ttcctcacaa aaacctttta acccattata
      121 tactctctgc gctcttgaat aatgttgaca caaggcaaaa acggttggtc cgcttccact
      181 catcaatgca gcatctgctc cgtacttgat catacgctct ttgatttgat ggatgactgg
      241 atgccgcttt cccgttatat cttctaaact gtttcccatt tttttgatca tctgctgata
      301 atcttgctct cggatggctt cagcaagtcc gtggatgtct tgatgttgga tggcttgcgt
      361 atctacttct ctaaacactg tacgtgtaga gacactcatt cttggtttga ccaacacaac
      421 ccagcactgt ggcatagaag gtaatacttg gatttcttcc cctttgccac cgacaaatgc
      481 cgtacttccg tagacacagt aaggaacatc tgtaccgact tccaaaccga tttctgccag
      541 ttcttctaac gaacagccca gattccataa ttggttgatc cctcttagag cagctgcaca
      601 gtctgtactt cctccgccta atccagctgc tactggaata ttcttcttaa caaagatctt
      661 gacaccggtt ttgatattgt atttattttt tagcaataaa gcagcttgat aaacattgtt
      721 ccgttcatct accggtaaaa atgctttatt tgtttctaca atgattttgt cttctgttat
      781 ttcttcaaaa aatagatgat ctgccaagtc tacgctcgac atcaccattt ccaactcatg
      841 atacccatct tgccgtctat ataatacatc cagtcctaaa tttatcttgg ctggcgcttt
      901 ttcaatcaat tccatcctct cacccattcg aaaagtctaa gtcgcaagta ttctatcata
      961 gggaaaaata aaagtatagc ttatgtctgc ctagtggttg attttgtcat cgtttcaaca
     1021 gctttcacag aattttctct tcataatcca aacaagttct agtgattctt ataattctgt
     1081 gagcacttct tctgccaaac tcggaacatt tcctttttct tttcctatca gactgagtag
     1141 ctgcatggta gcaaatccga aattacttcc tttcctataa gcaaaataac gtccttccca
     1201 atgatcgaca tattttaatt tcttttcata agcagactgg aaggaataga aaaaattacc
     1261 ataattatat acgatatgca tgactttttc ttgaaagaaa gaaaaatctg tttctccaac
     1321 attagctaat ggagctaagc ctagattggc ttccgagtag cccattttct cgtatacaat
     1381 aaacagattg gctaacagat agtcagctaa ttcctcagat tctttaggta agacccgcaa
     1441 caaatcatat cccgcctgct tcttcgtcat cggctgagcg gtgatgaagc caacgacttt
     1501 ttgatcttta cggacaatac cgacaccgct agataataca tatgatttat caaatcgtcc
     1561 cacagagaag tatttttctc gtgcaccttg aagccatttt tgtgaaactt catttacagc
     1621 ttgatgtaat tcttccggca gttgatcgta ccaagtaaaa tggtatcctt ctttcatcaa
     1681 acgttgatat tcaagttgct gactcagccg agacaagtct tctgttgcta gatcaacaat
     1741 ccctgcttca cctactttcg tgaagtgaaa acccaattca tgaagcatca ctacgtagct
     1801 ttctgatatc ttataaaacg caagctgata gcctaaacaa tctgcttgtt tcataaactg
     1861 attcgttgct gcccgcatca ttttaggatt gccaactgga tctcctaaaa caaagcattt
     1921 gtttcctttg atctgataac caaaaacgac ttgatcttgg tcgtcttctt ggtaataaaa
     1981 ataactgtaa cctgacaagc gaagctgatg gcttgtagct gtgcctccgt atgtttccaa
     2041 taaacggtca aaacggtctt tttcgtactc actgccaagc ttcgtagtat cttcagctaa
     2101 atattgataa agcgccacta aagctagcaa tgagatgccc aaaccaataa gccctgagaa
     2161 ccacacatca tctgaaggaa acaaaataaa atgatttcct gttattcgac cgactaaaaa
     2221 gcgtccgctg tgatagcctg caattgcgta aacgataaac aaaaagccga acaagctagc
     2281 atcaaagatt atagctcccc aagaataaac gaattttgtt cgataaaatt cttttctgga
     2341 taaccacaca gctaacagaa tcaagacata taccaatatc aactgccaag ataacgtacg
     2401 tgtgattgta tttaaaatac caaaagatag taaagcaatc gtaggcagaa atgccttttt
     2461 gacttttact gaaagtgctc tagcaaggcc gagtaataaa aagccaacca atagattcac
     2521 tgtctgatct aaaaaattga atgagaacgg caataatact tgaaatagcc gactgattgc
     2581 agacagatta gtgattgttg atagaagtac catcatgatc cccgcaaaat agacagcact
     2641 tacgagcacg atatgcgcaa ccttttggaa aaacagtcgc ggaatgttat ctaaaaatcg
     2701 attgattttg actcctgtat gtgtgatgaa caatagaagt cccgtcataa aaggtagaac
     2761 ataataaaac aaccgataat ataatagcca aactactgct acatcttgcc gaacagacaa
     2821 ttgactcatt cctaagatca tcaaaacgtc aaaagtcccc attccacctg ggaccatcgt
     2881 cagcattcct atcaatgtag caatcatgaa cataggatag atcgaaagca tcgaaatatc
     2941 tattctcatc aacttgccga tgatcaaaaa taccgcaagg gctcccgtcc attgtccaaa
     3001 ggatgccagt agcagaccaa gcaagccttt tggataaaac tcgaagaaca atgtctttct
     3061 ctttagataa gcaaaaaaga aaatgactgg tgctaaaaaa ctaccgccga aaagccagat
     3121 ccaatagctt cgaaaaatac tttctgactg aataaaaaag acatcaataa atgtaagaaa
     3181 cgaccataca gacaaaccag tcagcataaa tagtgcgact tttgatacag tggctaaaac
     3241 catttttcta tttgtctgct ttccataaaa actagcccgc agacttgctc ctaccacacc
     3301 accaaaaccg gcgagattgt tgatcgtgtt tgttacccac gcagcaagaa gaaactcttt
     3361 tctttccatt tttggttttc cttgtttttc taagactctg atcgtcaccc aatcatacaa
     3421 caacatcgga gtgacaccca gcagtccagc cagaatcatg agcaggatcg tcatgcggct
     3481 ttgttgtccc atcctttgcc aaatatcttg ccaactcatg ccattagcaa tatttgcgac
     3541 ttgatttgcc acaaagacta acacggagcc gaaaaaaatc agttttaaaa tcagactatg
     3601 ttcctttatc cattgcagca tcaagtatcg tttatttttc acattttccc attcctaact
     3661 tgtatacttt tgtgtcatac cccaatgtta tcttatttat tttaattttt ccactttaga
     3721 cctgttcttt gcttgcgatg atagaaaaag aaagatatcc taatgctaaa ggagaggata
     3781 acatgaaagt aggatttatc ggcacaggga atatggcaca agctattatt gatggtttat
     3841 taaaaaaagg aacagtctcg aaagaagagg tgttcgtttc cagtggacat tttgaaaatg
     3901 cacaaaaatt tgctgctgaa actggcgtcc atgcatgcaa aagtaatcag gaagttgcat
     3961 cacaatcaga tatcgttctt ttagccgtga aaccaaaaat cattgaaaaa gtattggaag
     4021 aactaagaga atatagccag aaagtcttat tcatttctat tgcttcagga aaatcattaa
     4081 tagatttaga aacagcgctt gaagatcctc aggcctccat cgtacgtgtg atgccgaatg
     4141 tcaatgtcag cgtaggagca ggcgtttctg cgatttgcaa aaatgaagct gccacggagg
     4201 aacagcttca gcaagctacg atgatttttg aagcaatcgg cagcgtctat cctttagcag
     4261 aatcagattt cagtacattc attggccttg ctggcagctc acccgcattc gtttacatgc
     4321 tgatcgatgc gatgggacgg actggtgtac tgaatggttt gtccaaacag acagccactg
     4381 agattgctgc aaaagcaatt atgggttctt gtcaaaaatt cctttcaagt gataaaaatc
     4441 catgggaact tgtagaccaa gtttcttctc ctggcggtac aactgttgca ggtgtagttg
     4501 ccttagaaga agcaggattc atcccagcaa tcatcaaagg aatcaatacg acgatcgaga
     4561 aagatcttga gatgttgaat aaaaaataat gatagcaatg ttttttcgtg ccttacaaaa
     4621 aaagaagagc tgtggctttt gccgcggctc ttcttttgta ttttttatta tttagcataa
     4681 tcagttgcac gagtttcccg aataacgatt actttgatgt gtccaggata atccagttct
     4741 tcttcgattc gtttacgaat atcacgtact aaacgaacag cgtctaaatc cgagatttct
     4801 tctggcttga ccattacccg aacttcgcgt ccagcttgaa ctgcgtaact tgattctaca
     4861 ccggcgaagc tgttcgaaat attttccaga cttcttaaac gacggatgta gttttctaat
     4921 gattcacttc gcgcaccagg tcttgctgct gagagcgcat ctgctgcagc tacaaggaca
     4981 gagataacag aagtagcttc cacatctcca tgatgagaag cgatcgcgtt gattactact
     5041 gggttttctt tgtacttggc agctaattcc gcaccgatct caacgtgaga tccttcaatc
     5101 tcatggtcaa gtgctttccc aatatcatgg agcaacccgg cacgttttgc taattggata
     5161 tcttcgccaa gctctgctgc taagatacca gctaattttg caacctcgac cgaatgcttc
     5221 aagacgtttt gtccataact agttctgaat cgcagacgtc cgatgatctt gattagatct
     5281 ggatgaagtg tatgagcacc gacttcaaac gctgcctctt ccccgtattc gcgaatacgt
     5341 tcatccattt ctttacgaga tttctcgacc atttcttcga tacgcgctgg atggattcgt
     5401 ccatcttgta tcagtttttc caaggtcatc cgtgcgattt ctcttcggat tggatcgaat
     5461 ccggaaagaa caactgcttc cggtgtatca tcgatgatta aatcgatacc agtcagcgtc
     5521 tcaagcgttc gaatattacg accttctcgt ccaatgatac gacctttcat ttcatcattc
     5581 ggaagggtaa cgactgaaac agtcgtttct gaaacttgat ctgctgcgca gcgttgaatc
     5641 gctaatgata ataagttttt cgctttgcga tctgcttctt ctttggcacg ctgttcagat
     5701 tcttttacca ttagagttaa ctcatgattc agctcttctt cagtcgattt catgatgaca
     5761 tctcgtgcgt cctcttttga taaaccagca attttttcta gttctttttg ttgatcttca
     5821 atcatttttt caacgtcttt ttctcgctct tcaattaatt gctgttttga actaagtgct
     5881 tcttctttac tttcaagtga gttctcgcgt ttttctaacg agtcatcttt acgattcagc
     5941 gtttgttcgc gttgaatcaa acgattctct tgagatttta attctaactt actttctctc
     6001 agctcacttt caacttcaga acggtatttc tgattttctt ccttagcttc taacaatgct
     6061 tcttttttta gagtttctgc ttcttttttt gcactatcga tgattccagc agctgatgat
     6121 tgtgcaccag ctatttcttt ctcgtgacgt gactttgcga ccataaaccc taaaccaaga
     6181 ccgacaatta aaccgatgat agcgaggaga atgttgaata ccataaaatc cacctccata
     6241 ctatcttttt catttttatc atctatacag cttctatgat aaattattct aaactcgtca
     6301 atatgcatct tcgcatatat gtgtacaaaa aacatgcaca actttattct aatgtttgta
     6361 ccccgtagtg tcaactttaa ataaacaaag aaacggcttt ctacttattc attaaacgat
     6421 ggtttttaaa ataaatgaca ctagtttcat ataggtaaac ctttaaattg agaaactttt
     6481 ctattaaatt attctttgta gatgaacaga tatttttatt ttatgtaccc actcgtataa
     6541 ttaaataact cacgatttct ttattcaata aaaaaacggc aggtcaagga gagtacctac
     6601 tcttcagacc agccgttttt gtttatcata atttttattc ttctactaaa ggcaattctt
     6661 cttgtccttt ttcatcttct gggacagcga cttcttcacc aataccataa gctgctctaa
     6721 ctagagcaga aacttctgcc atcatttcag gatgattagc catatagatt ttagcgtttt
     6781 cccgtccttg accaatccga tcttctttat aagaatacca agcaccgctt ttatcaacaa
     6841 tgtctttttc tactgccatg tctagaagtt ctccttcttg tgagatccct aaaccataca
     6901 tgacatctac ttcagcgatt ttgaaaggag gtgctacttt gttttttacg actttgatct
     6961 ttgtacggtt accaacaata tctgtacctt gtttcaattg ttctgcacga cgtacttcca
     7021 aacggatcgt tgcgtagaat ttcaatgcgc gtcctccagg agtgatttct ggattcccaa
     7081 acatcacgcc tactttttca cggatttggt tgatgaaaat agcgattgtt tttgttttgt
     7141 tgatcgaacc agagagttta cgcaatgctt gagacatcaa acgtgcttgt aacccgacat
     7201 gtgagtcacc catttcgccg tcgatttctg ctcgtggaac taaagcagca actgaatcga
     7261 ctactacgat atctacagcc ccacttgata ctaaagcatc agcgatctct agaccttgtt
     7321 ctcctgtgtc aggctgtgaa agaagtagtt catcgatatt cacacctaat ttttgtgcat
     7381 attgcggatc taacgcatgc tcagcatcaa tgaaagcggc cgttccgcca tttttttgta
     7441 cttctgcaat agcgtgtagt gcaactgttg ttttacctga actttcaggt ccatatactt
     7501 cgatgatacg tccacggggg taaccgccga cacctaaagc gacatctaac gcaagagagc
     7561 cacttggaat tgtagagatt tgttggtcga ttttttcgcc caatttcata atagagcctt
     7621 taccatagct cttttcaatt tttttcagtg cagcatctaa ggctgcttta cgatcatctg
     7681 ccaatacgat aatcctcctt atatttacaa atcagagcaa catttcatta tccttatcat
     7741 atctttttta gtgaaaaaaa gcaagaaaaa accgaacaaa tattcgcatt tatcattttc
     7801 ttaaaagctg ttttctcaaa agatcaaatc cttgcataac cgcattatat ctgatagcag
     7861 cacgatctct ggcaaagtga tatttttccg aaaccacgcc gtctttagat gaaatagcga
     7921 tccatactgt tcctgcagat tgaccttcta acgtgtccgg tccagccact ccggtgaaag
     7981 ctacactgta atctgttctt gctatctcac gtgcttgaat ggccatctgc tccacacatt
     8041 ctttgctgac tgttccatat tctgctaata gctccggatc aatctccaga aaaccagctt
     8101 ttgtttggag cgagtaggta acgaatccgc ctggaaatac ttcagataca ccagcaatgt
     8161 cgcctaatgc ggcttggaag gcgccggctg tcaaactttc cgctgctgtt acagtctgct
     8221 tgttttcttt cagtagttcc actacgactt ttgctagcga attatcatcg ccgtatccat
     8281 aaaaatattc ccctactcgt tcttggattt tttcttctaa tgctaaaaga gcttgattcc
     8341 ctgcctgaac atcatttgtt ttcactgtca gtcgtaatgt cacttcattt ggtttggcat
     8401 atggtgcgat cgttggattg atctgtgtct caatcagatc ttttagctca gtgaccagtc
     8461 gcgattcacc aatcccataa aagcgcaaaa ctttagaaat tagtttttct tcagaaggaa
     8521 aacgttcctc tagtaaagga cgtacttgtt ccacaaacat tggtttcaac tcattaggcg
     8581 gaccgggaag aaggatatac gcatgtgtat ctgtttgata aaaagtccct aaagcaagcc
     8641 ctgtacgatt tggtagagga accccgcctt cgataatctg ggactgttgc agattgtttt
     8701 tggtcatttt tcgatgggtt gtttcaaaat atgccaataa ttttttgtat ccttccgtat
     8761 tttgtatgag agacttaccg aggtgagccg cagttgcttc ttttgtcagg tcatcttctg
     8821 tcggtcctaa tccgccacat aagagtatga gttcacttct tgtttcagct agagcaatca
     8881 actcttctaa acgctgctga ttatctccta caactgtttg ataataaact tcgataccta
     8941 gatcagctag ctgttctgac aagaaagtgg cattcgtatt cacgacttgt cctagtaaaa
     9001 gttctgttcc gacagctatt atttctgctt tcattacttc ccgctccctt atatagtata
     9061 cgctgtattt ttatattttt ttatttaaaa agagggcaag gagctgtgac aaaaatcttg
     9121 tcacagctcc ttgttccaaa taaacagtgt tctcctgcca aaacctcggt cataagtcac
     9181 aatatacata aaacatgaaa agctgttttt tgaaattatc ccttgtgact cagagttaga
     9241 cggcaggctc acaacctctc cactaaacga tacttacatt gatcctttga acacaccgct
     9301 gtttttgatg aagtaatcca cacctgaata gatagtaaag atcagacaag cataaagcat
     9361 gatttgatct actggaatac cgatcaggct gaatgggata ttattgataa acagcaagat
     9421 aatagcaacc atttgtgtag ccgtcttgac ttttcccggc caagctgcag ccatgacttc
     9481 tccatgttcg acaagtagta aacgcaagcc tgtgacggct aattcacgac agacaatgat
     9541 cgctactacc caaccagggg cctttccttg gtcaaccaac atgataaaag cagtcataac
     9601 tagcatttta tctgccaacg ggtccgcaaa cttgccaaaa ttagtgacaa ggcctcgcgc
     9661 acgggcaatt tttccatcta accagtctgt gatactagct aaagcaaaaa taactgcacc
     9721 tactagctgt gtgacagcca gtgtggtgtc tcctacgtta aattgtcccc aatctaaagg
     9781 taaacctaca accaaaatga aaatcgggat catacaaatt ctaagtacag ttaatttatt
     9841 tggtaaattc acactatcga ctcctttctt ccctattcta cgtgactatc agcgaagttt
     9901 ccaccgatat tatttacttt tccaaatgaa tttattcaaa ggagtatttt tattgaaatt
     9961 gatctttttg aattaacgaa gaaggaccgc gacacagatt tgtcacgatc cttctttttt
    10021 atacctacaa gtaaacagat gcgtaaacta atttatacga tcattctgcg tattgaagag
    10081 taaggttcac attttttgga ctctgattgt tttcccctgg attaactggt acggattctc
    10141 cattcgcttt gatcgttgca ttgcttgctg caccaatcgt gatggtcgca tttgttgctg
    10201 tttccggtaa cacggtactc tgtgtttcat ttgctgccag tgtcccttga taaacatagg
    10261 cattgtctac tagcacacct aaccatacac gattgacagc agtaaattca agggttacag
    10321 gcttggtagc gtctgtgata tttactgcga tggcactgcc agaatcttct cctgtagcta
    10381 ttgccatttt cttttcttca cttgattttg ttgttgattc tactgtagaa gactctttgg
    10441 tctgcgtgct ttgctctgag gaagaggact cctcgactga tccttctact ttaagcgaag
    10501 aagctgtgtc tccgattatt ggatcagctt gacgatcttg ccaagtcata tagcccacga
    10561 caacgacaat cgccaaagca accaatccta ataaaatgac aggaagactt gtccaaaagc
    10621 gacttggatg cgattcttcc tgatgcattg cttttctaga agtacttact gtttcaggct
    10681 gcggacgttc agggaccgat cctgtcaatt tgcttttccc atcaaaaaca tcgactaaat
    10741 ggtctccatc ttctcctaca gcttcagcgt attgccgaat aaatgcccgc acgtaaaatt
    10801 taccaggcaa ttggtcaaaa tcatcattgt cgattgcttc caaataacgt ttttgaattt
    10861 ttgtaatctg ctgtaattca tctaaagata tttttttatt caggcgtgct tgccgtaatt
    10921 tttctccgat ggtttcaaaa gccactcgtt catcttctcc agtctgtttg attctccgaa
    10981 gcatctgctg ttgcaacgaa agacgttatg tttgggtgct gtgacaaaag tcatgtcaca
    11041 gctccttttt tccgaataaa cattgttctc ctgccaactc ctcggtcaca agtcacaata
    11101 tccaataaat ataagaagct gttttttgaa cttgttcctt gtgactcgga gttaaacggc
    11161 aggctcacga cctctttctt aaacggtgtt caatcagctg ttcctcggta ttagctcaaa
    11221 aaccggcaaa atataaggag atatttttcc gtatgtttct ctaatactca ggtcaaaacg
    11281 ctgatttcac aacctcttcc ttaaacggtg ttcaaaagct tgcatctgcg gtaataagtc
    11341 caaccaagtt aaaaacatta agaagctgtt tttacttggc tgttcttatt acttgatgca
    11401 gggcagcttt ttcacaacct cttggtacac ggtgttccag aagtagcttc ttcggcaata
    11461 agccgaaata tcacaaaaat ttgagaagca attttcgtaa attccttctt atttcttgaa
    11521 gctgaccact tctgtcacaa cctctttgat tacttcgttt gattttattt taatataagt
    11581 tgattaaaag agagcatgat ttgttttatt ttctcactaa tttttccaac acaactaaga
    11641 ataccataaa agctgatttt ggaggaggtt cctactacaa gtctaaaacg aaatttaaga
    11701 cttttcctca taaaatactg gtttattttg gtctcatgta aaactcgctg aaggcctctt
    11761 cacttataaa tgcttcccca gctgccagta cgtctttcat ttggatcgaa tcgatgattt
    11821 caggaagatc aaataaagtt cgatcgccaa ataaactttg cgtaaattga ttggcaatat
    11881 attcgatcga atttaatgat tggaaatatt gtccaagcat tttcttcttc agcaaatcga
    11941 gatttgtttc tgacacttcc ggatcatcag cgaatccaag aatgatttct ttgacttttt
    12001 ctgctgcttt ctctggttcg tccgtatctc cgctgaagtc cgcaaagtga aattcacgat
    12061 ccaagctgaa ttcaaaacca aagctgtcat cgataattcc ttgattgtac atcgctagat
    12121 agtttcgaga agtattgcct aaaatcattt ggaacaacag attgattgct gttttatagc
    12181 gtaacagttc tgttccttct tgcggcaatg tgtctaatcc tttaatacca agtacaaatt
    12241 tatctcgtgt aatggctgct tccaatgcac tttgtttgat gatttctttt gtgttttcag
    12301 ggaaataacg tacaatttct tgctttggag ggaaattttt tgcttcttgg ttttcccgaa
    12361 taagtttcat caatttctca ggctccatct taccaactac aaataaaacc atattgcttg
    12421 gctgataaaa cgtccgatag cacgtataca agtcttgagc tgtgattttg tcgatacttt
    12481 caaccgtgcc cgcaatatcg atatgcaatg ggtgtgtagg atacaagtta tttagtatgc
    12541 caaaaaacat ccgccagtta gggtcgtctt catacatttg gatttcctga ccgatgattc
    12601 ctttttcctt gtttacagtt tcttctgtga agtaaggtgc ttgtacaaaa tcgatcagag
    12661 tcgtcagatt cttttctacc tgatccgtcg tagaaaacag atagctggtt ttcgtaaaac
    12721 ttgtgaaagc attggcggaa gctccttgtt tcccaaataa ttgaaagaca tcaccatctt
    12781 ctttttcaaa caacttatgt tctaaaaaat gggcaattcc atctggaact ttcacctttt
    12841 ctttttcacc atagggaata aattcgttat cgatcgaacc ataattcgta ctgaacaaac
    12901 catatgtctt gtgatagtca tttttaggca acaaatacac ggtcaaaccg tttggcagta
    12961 cttcatgata aagtgtttca ttgatttgct catattctgt tttattcatt tgcatttcct
    13021 cctgctaaaa agaagattgc ctgcaattcg atttgctcag ctacttgttg tatctctttg
    13081 atcgttacgc tctccatacg tttcaaccat tcttcatcag ataattttgt ttccggcagc
    13141 cagctgtcca aaaaagaagc ttcgattgcc gcttgcggag agtctagaga aagcagatat
    13201 tggtttctca gcattgcttt tgtctgagac agttccaact ctgttatttc accatttcgc
    13261 aaactttcta attgctcgtg aatcagatga agtacttgat ttcggttctt ttcatcgata
    13321 ccggtttgga cactcatgaa tcctcggaac gtatccacgc tactagaggc ataataagct
    13381 agactttctt tctctctgac attcatgaaa agtttagagt gaggaaaacc gccaaataaa
    13441 ccgttaaaga ccattagcgc aaaacgttct ggttcatcgt aatacacatt tgtctgataa
    13501 gctaggttca gctttgcttg aacgattggc tcacgtactt gtcgttcctt cacgatattc
    13561 acttgaggtt gggtataaaa cagatctgga taaactgttt cccgcgactc aaatggtaat
    13621 tgcccaactg cttcagctgc tttgtccgga tcaacatcgc cgacaacaaa aatgtcgatt
    13681 tgatcatttg ccatcatttc ttgatagtag gacgctagcg aactagcagt gatcttgtcc
    13741 aaatcttcga ctacgcccaa ccctgggatc ttttgttccg ggctgttttc aaaataaagt
    13801 tcttgcaagg cataggaagc aaaagtttgt ttatcttcct tcaaactttc caaatataga
    13861 cgcagattgt tcttttccaa gtcaaacgtc aactgatcaa actgatgatt tttaatattt
    13921 ggataaaata aaacctcttt taaaaaatct accgcttgag aaaaaaggtt tggatcattg
    13981 acatattcac cattcacaaa actgatccct gcgtttagcc aatgtaaatt tccttttttt
    14041 ctaactgata atccaaagct tgcgccataa agctctgcaa gttgcgcact taactttgtc
    14101 tgatccgaat aattcaaact atttgtttcc aacatgctgc tgagaagtga acgtttcgtg
    14161 atgacttctt gttgcagacg cgcagtaaaa cggataaaaa tccggatcgt tttgtattgt
    14221 tttgttggta agacatgaag attcacgcct tctgccaatt gatacaccat atttatatgc
    14281 tccttttcac agttgattgc ccgcttactc ttttaatggg tcaacctgcc ttttttactt
    14341 attcctctgc ttgaaattta taagttcagg caattcgccg ttttttctaa atgatgttac
    14401 actttatagt atagcataga tgttttttat caaaagaaac agacggctaa aagcgtatat
    14461 tgttttttta ttttttattt ttcgatatac taaagacaaa taaaagaagg gagggtcatt
    14521 catgatcaaa gaattcaaag agttcatcat gcgtggagat gtcttggatc ttgctgtcgg
    14581 cgttgttatt ggtagtgcgt ttacaagcat cgtaaaccaa gtggtgcaag gcttgatcac
    14641 accactaata ggttggtttg tcgcaatgat cacaggtact tctgatttag aaggagcact
    14701 ttcaattctt gactggtcgc cgacaaaagg cgtcacattt gcgtttggta acgttattag
    14761 tgctatcatt acatttctga tcactggttt tgtcttattc ttagttgtca aagcggcaaa
    14821 ccgtgcgaag aatttacgct ccaaagaaga gcaagtggaa gtggaagaaa ctcctactgc
    14881 agaagattat cttagtgaca tacgcgatct tcttgccaaa caagtggcag aagacaatac
    14941 acatccagta gagcaaacaa atctttccga tacttctaaa taataaagaa tctaaaaaaa
    15001 aagaaagccg attttcagtg tcggctttct ttttttagat tcttaacttg ctacttgttc
    15061 ctttttcatg tcgatatgtg gaatatcatc ttccaaatac acatctgaga ttgccttgaa
    15121 tccaaacgac tcataaaaag cttgtaggta ttcttgtgcg ccgatcacga ttggtttttc
    15181 tggaaaattt ttctggatca tgttaatcgt ttcttgaaca atctttcttc ctaaaccatc
    15241 tttacggttg tcctgatgaa caaggacacg tccaaaatgg atactctccg cttcttcata
    15301 tactcgcgtg taggcaagga tcttcccatc agtatcttga aaaaacgtat gccaagcttc
    15361 tccatcaatg tcatcaactt cttgataagg acactgttgt tccacaacaa aaactgcgat
    15421 tcgttctttg acgatcgcgt aaaactctgc tgttgacaat tcttcaaatt tctttacctg
    15481 atattccact aagcaaacgc cccctgcttt tttattctta gcataccgat tcgcacacaa
    15541 aaaagcaaac gaaccttacg aatctcaggc tcgtttgctt tttaaactgc ttcagttaaa
    15601 aattcgatct caactgtccg agtaagtaca tctgaatagc tgtaagatac tcgttcaaaa
    15661 gaattttctt caggatccaa atcaacaacg aatactgaag gataggtttc tgttaaaacg
    15721 ccgcggcgtt ctgtttgacg ctttcttcct gtctgagcaa ccaacatgat tgggctgccg
    15781 atacgacctt ctaaatcttt cttgattgaa gctaaagttg ttggcataca gttcacctct
    15841 actatcaact ataccacata tttttaaaat tttcaagttt accataaaaa aaaaaagaaa
    15901 tgcaataaaa ataaataaga agctcattaa gatttttcta aattattcct cattataaaa
    15961 tttcaagtgt ttttttattt tgagttttac tctcccgtaa gcgctcatta cttgcttgtg
    16021 atccattgct aaacattcag ccatgtctac aaaatcaacg ccttgcatat aataatgaaa
    16081 aattttagct tctatttttg aaaactggat ttcgcaattt tcgattgctt cttttaataa
    16141 aagatgctga attgctaaag gttcttgcaa tgtgatctct gctaaaaagt ctggtccttc
    16201 tgtctctatt ttttggtcga ttgagacttc atctttttga tcacgacgct tcagcgcgtt
    16261 ctgcttcctc aacagagaat agatttgatt gcttataacg cttttataat aacttccaaa
    16321 gctaacacct ttgctttttt cgtaagttct caatgcctga tataaagcga tccttgcttc
    16381 ttgttcccaa tcctcagaaa cataatttct taaataatag gtgtttttca aacgtagtaa
    16441 aagtggaaaa tatttttgat acagggcagc aaatgcttct tcgtcaccat tacgtgcagc
    16501 gcgcaataac tcctcagaca taaaataatt cctccataaa ttcgtttatg gaggaatctt
    16561 atcatcaatg attcatcgaa ctgattagaa cgatatgtta tttatttttt ctcattaaat
    16621 cgtccaattt ttgggacaat ttttttaact gctcttcatt ccatggagaa ttacgtcgga
    16681 aatcaataaa atgcatatct ttattttctt gggctattgt tttttccgtt ttttttatcg
    16741 tcttatataa ttcataagca gaagttcgta atgctccttg ggaaaagatc atccactgtt
    16801 ctgctaaatc gcttgtcgcc actgtcactt gtgtcaaacg gtcattcagc tgaccagcga
    16861 ttctctcgat ataactgtca gccgtttcgt cttctttcgt aaaaacaaca tccaactggt
    16921 atttcttata attttgttgg atgcccggaa ctagttgggc atcgaagaca acgatgactt
    16981 ccattccttc atatttcgca taatttgata aacgatgtaa aagagcttct cttgcatccg
    17041 ccattttgtc ctgcttttta agctggacaa gttccggcca cgaaccaatc atgttgtagc
    17101 cgtctacaat caacagctgt tttttcatgg tctactcccc ctgacgttgg gaaaaagcct
    17161 gatacataag caaaccagca gcaacactgg catttaagct ctggacgtgc ccggtcatcg
    17221 ggatcgtcag caattcatcc atttcttttt tcaatcctgg actcattcct ttgccttcat
    17281 taccaatgac taaagcaatt gatcctttgg cattccattt ttggaagttt gtgccggtca
    17341 tatctgtgcc aaatacccaa aatcctgttt ctttgagctg ttgcacagct tgtaccaaat
    17401 tcgtcacgcg tgcaacaggg acatgttcga ctgccccagt ggaagtcttt gttacgatag
    17461 gtgtgattcc aactgctcgg tgttttggta tgatcacacc atcgacgcca cttgcgtctg
    17521 ctgtccgtaa aatcgaccca aaattgtgtg gatcttcgat gctatccaaa atcaaaaaga
    17581 aggggtcttc ttttttctct tttgttcgtt caatcagttg gtcaagggat aaatattcat
    17641 aaggtgtaat cgcaagaacg actccttgat gcacgccgtg atcgctcaaa gtttctaatt
    17701 tttgtttagg aacccatttg actggcacag cttgttcacg tgcgatttct ttcagttctt
    17761 ctactttttc tcctcgtgaa tcttcttgaa gaaagagttt attccctcgc ccttgttgta
    17821 gcgcttctac tgcggcatgg tgcccaaaga cgaaattatc ttctaatgct tcttcatttt
    17881 gatcagaaac tgactgattt cgtccagttt gctttttatt tgaatatcta ttttgttttt
    17941 tgtcttcaaa tccacgtttc gattgatgtt tgtttgttct ttctttaccc atttttttca
    18001 ccaactttct gaatacacca gcggatcaat tcttccatcc gctcggtttg ttttgtcaaa
    18061 tgcagatagc ccatgagcgc ttcgaaccct gttgcgaccc gatacgtcgt aacatcagca
    18121 ttttttgcgc tcgtatggct tttagcattt cttcctctgc gataataaag ttcttcttct
    18181 tctgtcaaaa gatgttcttc cagcatttgc tgaatcaaaa aggcttgtgc attcgcagaa
    18241 acataattcg ttgccgcacg atgcagctta ttgggttttg tttctccttg atcaatcagg
    18301 tggtcacgaa catagatttc gtaaatcgca tctccaacat aagccaaggc taaaccgttc
    18361 aattgtgtgt aatctctcat gcttctcttc tccatcgggt tccttgcggt gtatcttcta
    18421 ataagatccc tttttctttc aacaaatcac gaatttcgtc gcttcggaca aaatcacgat
    18481 tgcttcttgc ttgatttcgt tcttcgatca attgttcaat ctcttcatct aataactctt
    18541 ctgctgtaaa taaaataccg aagatcgaaa gccattgcga gaataaagct tctccttgtg
    18601 ccaagacagc ggcagaaact ttcggctggt ctagataagt gttcagccac ttagcaagct
    18661 catagaccac tgtgatccca tttgctgcat taaaatcatc gtccatttct tcgatgaatc
    18721 gctgttctaa attttctaac aaagcgattt tatccgcatc atctgccaaa ttctctgagg
    18781 cagtttcttg gcggaatttc aaattgtcaa acgtattctt cagtcgttgt aaattggtgg
    18841 ctgcttcttt catcgtcgtt tcactgtagc ggatcggacg acggtattgt gtggtcgcca
    18901 tgaagaaacg caagacttgt ggatcaacgt ttttgatcat ttcatgtaca gtgatgaaat
    18961 tccctagaga tttactcatt ttttcatcgt cttcgccaat cgttacatag ccattgtgca
    19021 tccagtaatt agcgaatttt ttccctgttt tcgcttcact ttgtgcgatt tcattttcat
    19081 gatgaggaaa ttctaaatct tgcccgccac catggatatc gatcgtttct cctaaatgtt
    19141 ttgttgccat aacggaacat tcaatatgcc aaccgggacg tcctttgccc catggtgaat
    19201 cccaagaaat ctcgccaggt tttgcttgtt tccataaagc gaaatctaat gggtcttctt
    19261 tttgctgctg ttcttcccct gtacgctgac ttgctccgac ttccagttca tcgatggatt
    19321 ggtcacttaa tttcccataa ttttcaaatt tacgggtacg gtaatacaca tcacctgaag
    19381 attcataagc atagcctttt tcaaccaaca cttgaataaa tgctaaaata tctggcatat
    19441 gatccatgac tcttggatgc agtgtagcag gttcgacatt caacgctttc gtatcttctt
    19501 caaatgctcg gatgaaacga tcagcaactt ctggtgctgt aatacctaat tcgtttgctg
    19561 tacggatgat tttatcgtct acatccgtga aattcgatac gaaatcgact ttatagcctc
    19621 gatattcaaa atagcgtcga attgtatcga aagcaatggt gctacgagca tttccgatat
    19681 ggatataatt atacacagtc ggtccgcaga catacatacc gacatgtccc ttttgtatag
    19741 ggatgaattc ttcttttttt ctggtcaatg tattgtagat ttttatcatt ctgatctcct
    19801 cactttttct cctttgattc gaacgacttt tgctggtact cctaccgctg tagcgtcgtc
    19861 tggaacatct ttcaacacaa cggcacctgc accgattttg gcgttttttc cgatcgttac
    19921 cggtcctaat atctgcgcat tcgcagagag catggctcct tgtttaacag ttgggtgtct
    19981 tttacccgtt tcttttcctg tgccgcctaa tgttacgcca tggaataaaa tcacatcgtc
    20041 ctcgatttct gcggtttcgc caatgacgat tcccattcca tggtcgatga acacgccagt
    20101 tccaatcgtt gctcctggat ggatctcaac accagttagg aaacgccaga attgagcatt
    20161 gatcttcgcc aataaaaaga gccggtgctg atataaaaaa tgagagaaac gatgccaaaa
    20221 caaggcatga aaaccagggt atgtcaaaac gatctcagct gtcgaacgtg cagccggatc
    20281 gtttctcttt gctgcatcaa tcgctcgggt aaaccaactc atgactttcc actcctttcg
    20341 taagaatttt atgtaggaaa tcatcttctt ataaagaaaa gaagcgcctt ttaaataagt
    20401 ttccttatca aaagacgctt tccgcgtggt tccacttttc ttcataatca atgattatct
    20461 ctgacagcta acgcctgtta tacgttcttg gctactttgt ttcctataaa acagtttttc
    20521 ctgttttctg tgtttcgcca agaccactcc cagaggcatt tcggcaacca ttcattgata
    20581 ccttgcacca cccggtatct ctcttgtaat gatcagctgc ttactttttt ctgttcatcg
    20641 tgtttagttc agtacttgat ttaaatgtgc taatgatttt tcacgaccaa gcaattcgat
    20701 cgtttcaggc aattctggac catgcatttg tcctgataca gctacacgga ttggcatgaa
    20761 aagatttttt ccttttacgc cagtttcttt ttgaacagct ttgatggcag ctttgacagt
    20821 tggagcatct actacttcca tttgttctag ctgtgctttg aatgcgtgga gaacagtagg
    20881 aactgtttca ccagcaagta cttcttttgc tgcttcattc aatacaggat gttcattgaa
    20941 aaatagcgta gacagatcaa cgatttctgc agcatagctc atttgaggtt gatacaggct
    21001 aacgatcttc ttcacccatt cgattgtctt ttcatctggg ttttctgcta cacgtccatc
    21061 agcgattaga taaggcagac acatgtcagt aaatgcatct agatccattg ctttgatgta
    21121 atggttgttt acccattgca gttttttcgc atcgaaagct gcgggtgatt tacttaggcg
    21181 ttttggatcg aacattttga tcaattcttg gtgttcgaag atttcgtctt cgcccacagg
    21241 tgaccagccc aataatgcaa taaagttgaa cattgcatct ggtagatagc ctaattcgcg
    21301 atattgttcg ataaattgca agattgattc gtcacgtttg cttagttttt tacctgtttc
    21361 actgttgatg atcaatgtca tatgtccgaa ggttggtggt gtccagccaa atgcttcata
    21421 gatcatcagt tgttttggtg tattggcaat atggtcatct ccacgtaaaa cgtgagtgat
    21481 tttcatcaaa tgatcatcga ccgcaacagc aaaattataa gttggcatac catcgcgttt
    21541 ttggatgaca aagtcgccac cgatattatc ggattcgaac gaaatagcgc ctttcaccat
    21601 atctgtaaat gcgtattctg tattgcgagg cacacggaaa cgtacaacag attctagacc
    21661 ttgtgcttct ttttctgctt gttcttcagg tgtcaagttc gcacatgttc ctgcgtaatg
    21721 aggcatttct cctcttgcgc gttgtgcttc ccgttctgct tctaattctt cttctgtgca
    21781 atagcatttg tatgctcgat tgctagcaag cagctgatca atcagcggtt gatagatttc
    21841 tttacgttca gattgacggt aagggccgta ttctccagga ttttcaggag attcatccca
    21901 attcattccc aaccaagcaa ggttttccaa ctggcttttt tcgccatctt cgatattacg
    21961 tttttggtca gtatcctcaa tacgaataat aaagtctcca tcattgtggc gggcaaacaa
    22021 ataattgaat aatgcagttc ttgcatttcc aatatgcagg tgcccagttg gacttggtgc
    22081 atagcgtacg cgtactttcg tcattttctt cctcttcttt caaactagac tttgactaac
    22141 agcaaaaatt ccgtcagtta cggcttctca aatatcattt atttaaagcc actcagcaca
    22201 aattttacct tttctaaggt aaatagtaaa gccaactggt tgaatttcct taccagttgg
    22261 ctgaatttct tttttatttt gattgtttag tctttttctc tgtctctttt ttattgtcga
    22321 ttcctcgtcc tgcatgtgca ggtttagcaa agatcatgcg tccagcagct gtttgaaggg
    22381 cacttgttac aacgacttcg atacgttcat tcatgtaatg ctgtccatct tctacgacaa
    22441 ccatcgtgcc gtcatcaagg taggctactc cttgttggcg ttctgtcccg gcttttacta
    22501 ctagaacatt catcgtttca ccagggatga cgactggttt tacggcattt gccaacgcat
    22561 tgatattaag aacaggtaca ttttggaatt cagagacttt attcaagtta tagtcattgg
    22621 ttacaatgac accatctaaa agcttagcca atttgatcaa tttgctgtct acttcactga
    22681 tatcttcaaa gtctccatcg tacatttcga cagatatacc ctcttctttt tgcaaggcat
    22741 ttaggatatc taatccgcga cgaccacgca cacgtttcaa actatcccct gagtccgcga
    22801 tgtattgtag ttcgtaaaga acaaaattag ggatcatcaa ggttccttct aagaatccag
    22861 tctttgtaat atcataaatc cgaccgtcaa tgatcacgct tgtatccaag attttatatt
    22921 tatggaaatt ttctcctgat tttcgttcta acagttgact ctcaacttga ctagctgttt
    22981 cttgctcgat ttttttgctt cgtgaaccaa aaactttctt ccattcttcg attcgtgtcg
    23041 ttcccattct caatcccaga tagcctaaga tgatcatcag taaaatcggc aatacactat
    23101 tgacgaatgg aatttccata ttgtaaagag gggtcgagat cacaacacct aaagtcaatc
    23161 cgatgatcgc accgatcgca ccgaagagaa gataagtcaa gctgacctca cttagttttt
    23221 gttctacttg tttgataata gctgagatat gtttggccaa cagcaaagat aataaaaaga
    23281 aaataagtgc gcctaacagg ctgttggtaa atgtattatt cagccatccg tttgactgtt
    23341 gaccgattgc ctgccaagct gagggtaaaa atgaaatacc caagcttgcg ccgacaatga
    23401 tcatcagcaa agtaatgaca cgtttttgca tcgtttttcc tccgtttcga caatcattta
    23461 ttagttagcg gaacacacgc ttcaacgttt cagccaaagt tgctactcca actatctcga
    23521 ttccttttgg cgcttcccag ttacctaagt tgtttttagg taggtatact tttgtgaagc
    23581 cgagtttttg gacttctttg actcgttgtt caatatgact gacacgtctg atttctccag
    23641 tcagtccaat ttcaccgatg aaacattccg ttggctgtgt tcctttttct ttatagcttg
    23701 aagcaatact gacagcgata gctaaatcaa tcgctggttc atttaatttt accccacctg
    23761 cagcttttaa gtaagcatct tgattctgaa gaagcaaccc agctcttttt tccagcactg
    23821 ccatgatcag agatacccga ttgaaatcaa gacctgttgt tgttcgtttg gcatttccga
    23881 acatagtagg tgtgactaat gcttggatct ctaccaaaat tggacgtgtc ccttccattg
    23941 ccacaacgat agcagagcca gttgcaccgt ctaggcgttc ttctaagaaa atctgtgaag
    24001 gattagcgac ttcttccaat ccatgctcat gcatttcaaa aatcccaatc tcattcgttg
    24061 agccaaatcg gttttttact gctcgcaaaa tccgaaaact atgatgttgt tcaccttcaa
    24121 aatataacac cgtatccacc atatgttcta acattcttgg gccggcgatc gagccttctt
    24181 tggtaacatg accgacgata aaaatggcta tcccatttgt tttagcggtt ttgagtagtt
    24241 ctgcagtggt ttctcttact tggctgacac ttcctgccac acttgtgatg tctggctgcg
    24301 tcatcgtttg gatggaatca atgataacat aatcagggga gatatgttcg atggcacgac
    24361 tgatttcgtt catatctgtt tcagcgtata gataaaattc tgtgttgata cttccgaggc
    24421 gttccgcgcg taattttatc tgctctgcgc tttcttcgcc ggatacatat aagacttttc
    24481 cgcctatggc tgctaattgc tgagaaactt gtaacagcaa agtggatttc ccgatacctg
    24541 gatcgccacc aatcaagacc atagaccctg gcaccacacc tccgcctaac acacggttca
    24601 gttcttctaa ttttgttttg atccgaggtt cttttttagg gacgacgtct gcgatttttg
    24661 ttggttttgc tttttctcct gtcaaacttg tccgagttct gcggtccgtt gtatcttgtt
    24721 cgatttcttc gaccatcgta ttccattttc cgcaatttgg gcagcgtccc aagaacttag
    24781 gcgacacata cccgcaattt tggcaaacaa attgtacttt tgattttttt gccattgtac
    24841 cgcctccttt tatcctaatc atgagacatt aaatcaagct taagtatctt taacgtttta
    24901 tttactggaa gaaccaaatc cgccttgacg ttgagcagtc gtttgatctt gatctgcttt
    24961 taaaaacggc aaaaagatcc cttggccgat acgctcgcct tttttgatca ctacatcctt
    25021 gaaaccaaag ttggtgaatt ggaacatgat atggccttca tttgcctcat tattgtaata
    25081 atcactgtca atcacaccta ctccgtttgt caacactaaa aaccgcttca acggattgct
    25141 tgaacggtta gcaagctgca aaaattcatc ttctcccatg taagctttga ttccagtagg
    25201 cacgagggtt ggtttcaatt gtttttcatt ttctgataca tcttttttca atccatctgc
    25261 gactgtttcc aacccctttt tccagcctgc tttccagata cttggaatca cgacctcttc
    25321 tgctgcttca aaatcatagc cagcagcatg atgtgtagcg cgctgcggta gattgatatt
    25381 ttgatcttta tatttagaaa taacttcaaa tcctcttttt ctcaccggtt ttcctcccta
    25441 aataaactac tcctattcta acatatccga ggctgtaaaa gactttcttt acaaattctt
    25501 caagaaattc tgctatctta aagtaaacca ttaattcgtt ctttttccaa acgactgcaa
    25561 gttgtacaaa aaaactattt tttacatgac tttactcgtc aaaacaagta gaatagaacg
    25621 aaattaatga gtggatattt gattgaggtg tcacatgcgt aaagaaatta aaaatgcaaa
    25681 acgaattatt gtcaaagtag gaacaagtac attgatttat cccaatggaa atatcaatct
    25741 tgcggcaatc gatcagttag cttttgtcct ttctgatctt agaaatcggg gaaaggaagt
    25801 gatcttagtc tcatcaggag ctgtaggcgt tggtatgcat aaattacatt tgtcagaacg
    25861 tccgactttg attcctgaac agcaagctgc ggcggctgtt ggtcaagccg aactgatgaa
    25921 tatctataat caaagattca gtgtatataa tcaacagacg gcacagatcc tcgtgacaag
    25981 agacgtggta gattatcctg aaagtcgtaa aaatgtgagg aatacattgg aacagctttt
    26041 gattatggac acgatcccta tcgtgaacga aaacgatact gtagcagtgg atgaattaga
    26101 tcatctgaca aaatttggcg ataatgatca gctgtcagcc atcatggcaa aacttgtcgc
    26161 ggcagacctt ttgatcgtcc tttctgatat cgacgggttt tattcagata atccgactac
    26221 aaatcccgag gctcagatgt atcgctatat cgataaaatc gatgaaaaga ttctggcaca
    26281 agcaggtggt gctggtagca catttggaac aggcggtatg cagagtaaat taaaagccgc
    26341 ttctaggata tttgaagcaa acagcgcaat gattttagca aatggaaaaa atccggcaat
    26401 tatttttgat attcttgcag gaaaagaagt aggtacttta tttaaggagg acttgaaatg
    26461 actgacttga tgcaattagg acaacaggca aaagatagcg cgcaacaact agcacttatg
    26521 cctacaaacc gcaaaaatga gctacttgtt caaatggcac aaaccatcaa aaagaatcaa
    26581 caagcgatca ttgaagcaaa tcataaagac ttagaaaaag cagtcgaaaa caatatcagt
    26641 gaaacgatgc aagaccgctt gcagctgaca cctgagcgaa tcaatgccat ggctacagag
    26701 atcgaaaaaa ttgcttcgtt agacgatcct attggaaaag tagatgagat gtggacgaac
    26761 accgacggat tgagaatcgg caaaaagcgc gtgccactag gcgtgatcgg catcatttac
    26821 gaatctcgtc caaatgtcac gacagatgca gctagtctag cattcaaatc agggaatgcc
    26881 gttatccttc gcggtggcaa agaagcattc ttctcgaatc aattattagt ccaattattg
    26941 cagcaagtac tggtcagtgc tggcgaatct ccttatgcca tccagtttgt cgatgatact
    27001 tctcatgaaa ctgcaggaca gttgatgcga ttgacagaat acttagatgt gctgattcct
    27061 cgaggcggag caaaattaat ccaacgagtc aaagaacagg caacggtacc tatcattgaa
    27121 acaggtacag gaaataatca cgtatatatc gataaagaag cacagttatc tatggcagtg
    27181 aacatcatcg tcaatgcaaa agcttcccgt ccatctgtct gtaacgcggc agaaacattg
    27241 ctgatccatt ctgagatcgc accttttttc ttgccagcta ttgaaaaaga actggtagaa
    27301 cacggggtct ctttacgtgc ggatgctcgt gcgttagaat atctagagac ggctgcttta
    27361 gcagaagaca cagattggga tactgaatat ctagattata tccttgctgt caaagtggtc
    27421 gattctttgg atgaagcaat tgcacatatc aatcggcata atactaaaca ttcagaaacg
    27481 atcgtgacag atagttatgc ggctagtcaa cgtttcttaa atgaagtgga cgcggcggct
    27541 gtctatgtca atgcctccac tcgttttaca gacggatcgg tatttggttt tggtgcagag
    27601 attggtatct ctactcaaaa gttgcatgcc cgtggtccaa tggggttaaa tgaattaact
    27661 tccacaaaat atatcattta tggcgatgga caaatccgcg aataatgttt cgtgtgaaac
    27721 attattcccg ccttttgcct tttttctgta aaggttctat ccaactagaa aggtgtcaat
    27781 atgtttgaaa ttttaatgat tttttttatt taccttattt ctctgaatat cgctgctttt
    27841 ctgggagtcg gtattttatc ccttttcttt caattcaaaa aacgaagcat agggagtcaa
    27901 cgggaaaaat ggtcgcagta ttttgacaaa atcgggcaaa aagggctggt tacacgcttg
    27961 cacattagtt acatggttgc attatgctta ttagcgacaa tcaattacta ttcttttttc
    28021 gatcattcaa tcgcttatac gattacgcta ctgattgctg ggatttttca cttatcttat
    28081 aaatatcagc tgaataaaaa ccacttgaat cggactttcc gctaaaaaat atccgtacga
    28141 taagtagagt gccttgaaga tcggcaaaaa actgccggac tttttagagg ctgtgacaaa
    28201 gattttgtca cagcctcttt ttccatataa acggcgttcg cctaccagct gcctggtaaa
    28261 tctgtttcct cttcgttaag aagcgaaaat ccgtcaaatg tttcgccatc ataataacga
    28321 agcagctcat ccatggacaa gttcatcgtt ttttcttttt cttcttccgc tgattcagcc
    28381 agtagctgtt cccgatacac ctgatacata ccagctattt tttgctgcat cgcttttcta
    28441 gtttcacctt ccgcattgat tattggcaat tctttgatat agccacgata aatcaaccga
    28501 tcatctgaaa attcaatggg taattcaacg agtgtcaacc cgacttcctg aaaatattct
    28561 tccagcatct attatccccc cattcttttt cttaaatgaa ttttagctta cctactgaaa
    28621 attttccagt ttcattattt tgagaaaaaa agggaatgtg acaaaaatcg tgtcacatcc
    28681 cctttttccg aataaatggt gttcttctgc caactcctca atcataagtc acaatattca
    28741 acaaacatga gaaactgttt tttgaaattg ttccttgtga ttcagagtta aacgacagtc
    28801 tcacaacctc ttggtaaacg gcgttcaatc agctgaccct cggtattagc tcaaaaaacg
    28861 gtaaaatatg agaagctatt tttccgtttt gtttctctaa tacttaggat aaaacgctga
    28921 ttttacaacc tctgttaaac ggtgttcaat cagctgtcct tcggtattag ctcaaaaaac
    28981 ggcaaaatat gaggaactat ttttccgttt gtttctctaa tactcaggac aaaacgctga
    29041 ttttacaacc tcctgataaa cggtgttcaa aagcttgcat ctgcggtaat aagcccaacc
    29101 aaacgaaaaa tatgagaagc tatttttgtt tggctgttct tatgacttga tgcaggacag
    29161 ctttttcaca acctctgatt tcttattctt cttcatttgt agcaaaataa ttacggtgat
    29221 aaatgataca acatacactg ataagaacaa tggcacctgc aaaataaaac gcatagcttt
    29281 caccaatatg ttcaccaatc acacctgcaa taaatggtgc taaagctcca ccgaaaaagc
    29341 ggacaaaact atagctggac gaagcaacgt ttcgttgcag acctggtatt tccatagcaa
    29401 tcgtcgtcaa cagtgtattg ataattcctt ggaaaaatcc tgctaacaca atgctgatcg
    29461 gtacaaggac tggcatagaa gttccagctc ctaataacag caaacaaata atgaataaga
    29521 caaatgctgc taaaattgtc cgataagtcg taaacttccg ctcaagagcc ggtgcaacga
    29581 aaatcgaagc aatagccaga agcacacccc agccaaaaaa tacaaaaccg acttctaatt
    29641 cagaatagcc aatcaataaa aatggcgcat acgcaagtaa agtaaagaat ccgaaattat
    29701 ataacaatgc gattagtcca actgaacgaa gtttatgatt gcttaacgct ttgacacctt
    29761 cccaaaggcc tacttttctc tttggtttct cagtttcagg taaaagtacc gctagggcta
    29821 taaaaccgca aaacatcaaa aatgctaccc caaaaaatgg ataacgccat gaaccactgc
    29881 ccaagacccc accgactaga gggccaacag ccattccgca gccaagtgct gcttcataca
    29941 tcatgattgc tttttctgtg ttaccaacta aaatactgac gatcgttgct aaagcagtag
    30001 agacaaataa agcattcccg acaccccagc cggcacgtaa cccaatcagc attccgatat
    30061 tcccagataa tccgccaaga ccagcgaata caacgatgat aaacaatcct gtcatcaatg
    30121 tttttttcaa accgatccga ctagataaga aacctgtaaa tagcatcacc acaccagtga
    30181 ctaacatgta actagtaaat aacagggtcg tttcagctgg cgtagcgttt aactgtgcag
    30241 caatcgtttt taagattggg tctactagac ccactcccat aaaagcgatc atacaggtag
    30301 ctgctacaac tagcgctgaa cttttcccct ttgtttcttc atttgtttgt tcttgtatat
    30361 ctatttgatt ctccaatatt tccatccctt tctctctatt agcatcatac atgatttatt
    30421 ttacatgtca atatgcacat gtaaaacgtg gaaactttac tgtgatataa taaagaaaat
    30481 aagacgagag gtaggatttt tatgaatact ttagcctata tattattatg cgtgctttta
    30541 tccaaaccac gttctgggta tgaattgaag cagctgatca caattttttg ggaagcccat
    30601 cacagtcaga tctatacaac gcttgccaag ttagaccagc agggttatgt gaccgttttg
    30661 gacaaagacg agcactctca aaaaaagatc taccacttga ctgatgaagg acgtgctctt
    30721 gtagaggaat ggatcaaaga ggaaactccc gctcctacgc aaaaagatga atttctagca
    30781 aaaatctatg ctttttcgac gctcgataaa acaacagcta ctggactgct attcactaga
    30841 gaacaacagc tgaacaaaat catgcaaaaa aatcaaaaaa aattgaatga cctctcgcta
    30901 gacaagaaac aagaatttgg acgttatgtt gtgatccagc gcagaattat gctttgtcag
    30961 caggaactcc aatggtgtca gcaagtaaga gaacaaatgc agcagttttt tgagtaaggg
    31021 ataaacagaa cctttctaga aatatctatt gaagagcaaa ttatcttcaa taaaagagca
    31081 atacagttgg aaaagaaaaa gcgatccgaa cgacaggcca aaacctatcg ttcggatcac
    31141 tatttttcta gaactgcatt caagagacta tgacaaaaag catgccaagg ccttttttca
    31201 aataacagtg ttccatcatt cattactttg aattgctttt agcacacttc ttgaaagcgg
    31261 gccaacgatg atcaactgca acggtaaagc ggcaatcaca ttcattttcc aagcattcat
    31321 ataagcagag aataattgat caggtattcc ttgttccatg atcacaccga ataaagacat
    31381 acatgtcacc attcctaaaa ccatcaagca tgaaatagtt aagatcattt ggaccttgct
    31441 ttctggattt actggtagtt taaaggcaat ttttttcgcc agcacaccga tgatgaagac
    31501 atccaaaatg aaagccacca cgaatccagg cactagacca atcaacaaac ttgataaatg
    31561 aagatcttga tgcagccata aattatacgc gctcatcccc aagaccatca aaaaacacat
    31621 cattgttgta aaaattattc cttctttttt atttgtaggc aatctgtacc actcctttaa
    31681 atttgtcacg aaaatcatcg tagcacattt aaaaatttct cgtaagtact ttttttattt
    31741 tctgcccttt tatttcataa acttcatcag ccgtacgctc gacaaagtaa gaatcatgag
    31801 aagtaaacaa aatcgttccc ggatatattt gcattagctt ctctaaagct tcgatcgttg
    31861 ccatatcaat aaaattagtc ggttcatcta acagcaacac attacttggc ttagtaaaaa
    31921 gcaaagcaat cgtcagacgg gtcgcttctc ccccacttaa tgtacataac ggtttcgtca
    31981 aagtttcctg agcaaaccct aagtgattca gcaaactacg gacaagcgat tctgaaaact
    32041 ctgtttgtct catgacaaaa cgaatgatgg attcttcaga tgtcatttga taaccctttt
    32101 gctgatatac ttgaaagctt actttcggag agagaaggat tccttctcct tgtttgcgga
    32161 tatgttctaa taaggttgtc ttacctgaac cattttcacc gacaatcgcg atttttttac
    32221 ctaaagaaaa ctgaaaatca ccatttacca gtaatgttct cccacttctt tccaattgga
    32281 cattttgtcc catgatggga tagcggctgt ggagttcaag agattttggt actggaaaac
    32341 ggatttgttt catctgctgc ggcttggttg tttctcctat tttttggagt cttctctcta
    32401 atgttttcgc attcttttgg atggcttttt gtacactgtc tttttgttta gaggaagaca
    32461 accgatccag tctgatttgt tgctttttct ttttactgga cactttttcc aacttttccg
    32521 cttgtttttt cttttcttgg atcgattttt ccaatcgttt tttctctttc atgatcgact
    32581 ctgcttctcg tagctgagtc tttttctcca attccttttg agtgagatag gcactataat
    32641 tccccgaaaa ctctcggatt tctccgtctt ttacttccca gattttcgat gctagctcat
    32701 ctagaaaata gcgatcatga ctaacaaaaa ccagcgtacc ataataataa cgaagctctt
    32761 caatcagtcg atcaatactt tttctatcta gatgggttgt tggctcgtct agaagcatcc
    32821 cttgttcata agaagaaaga agacgtatca actcgatctt tcgttcttcc cctccgctcc
    32881 atttccgctc tgaagtttct tccagtctta actggaaatg actggctaat tccaaattta
    32941 ggtcctttgc ttcagcgaga taggttaatt gaggcaggta gttgaaggtg atctctgttt
    33001 ggattttccc atgatcggga aaaagttcac cggctattag tttgagcaaa gtcgatttac
    33061 cgacaccatt ttctcctatg atccctatac gatcttgctg atagacacga agagaatcaa
    33121 tagatagttc tagctggttg ccaaagctga cttgtagatt tgttatattt actgctaaat
    33181 tttccatatg ttttacactc ctatttcatg taaaacaaca attatcgata ctaaaaaaga
    33241 gcagaaaaat ctgtttgcaa aaaaactgca tcacatcttt tcctactctg aacgaaataa
    33301 ctatgactaa atcaccaaat ctgtcatagc tttcacctat tctactgtcg ttcgaaaaaa
    33361 tggacatact ccatccatga aattgttgtt tatacatgtc ttttgaattt atttagttgt
    33421 tattcaaaag ttcgaaacgt aatttcatcg atgcagtcat ttttccgacc tcctttttta
    33481 ttaggttacc acacacgatt caaaaaaagc aatagctact gaatagtaac tgaaaacctt
    33541 ttattcgaac atagtgatct tttctggtgc taaacgaaca gactcgtaac cttcttctac
    33601 cgcttttcct attgataaaa taataactgg aacataacgg tctttatcca aaccaaatgc
    33661 ctctgccaac tgatctgctt cgaatccacc aattggattt gtttcatagc catgatcacg
    33721 cgcaactaac atgaattgca tagccgctaa gctagaatcg actttgacta catcgttcat
    33781 ttcttctctt gaaaaactct tgtaataagg aatgatcgca gctaattgtt gatctctgac
    33841 ttcttttggc atcttccctg attcgtaagc ttggttgtag atttcttcgc catattcgta
    33901 acattgcata tcgccaaaaa tcatgaccat tgcagaagaa gtatcatttt gtctcgtatt
    33961 gaaacggatc aaaggacgca agatttcttt tgcttcctcg ctttcagcga ccacaaagcg
    34021 ccatggtttc atattgacag aagaaggtgc agtcgtcgct tctttgatca tttccagcat
    34081 ttcttcgtgt gggattttgt atgtttcatc atatacgcga acggatcttc taccagtcat
    34141 aattgttgaa aaatcatttt tttgttccat tattattaac ctctttcgtt tctgttgtta
    34201 tattgcaaag ttgggtattc aatttatcta aaagtgttag aaattgtttc atttctacat
    34261 cgctgaaagc cggggatagc acggaacctg tatctttatg gtccatttca caatgcttga
    34321 ttttcttctg ggctttttct gttagcgcta catacacttc ccggttgttc tgaggatttc
    34381 tctgacgcga aacatagcca tttgcttcca ataccttcag atgacgagta actgctcctt
    34441 gatcaatctg caacttttct tgcaaactgc tttgtagcaa tggattttct tctttcagtc
    34501 tcatcatgat ctcgtatcta gtaagactta taccgatatt ttcttcaaag atctggacga
    34561 ttttttgatc ggctaatttg atttgatata aaaggtgact aatctcttct aaagacataa
    34621 tttcctcctt tctaataatt gactcgtcaa cgattgactt gtcaataata gaataaaaaa
    34681 agaaaaaagt caaagaatca gctgtgtaat aaaaaaaacg ctctcaatag aaaagtatag
    34741 agaaaagaaa ttttttgtag aatgaacgga aaaaatgata aagaaagcac aacgctaaaa
    34801 ccaagattat tgattcttat caaactatag aagatagctc ttctgcagta gataatttta
    34861 caccgccaaa aatttccgaa atcgcctttt gcaagaatcc cgtcgaatat tagaaattgg
    34921 atcagtatgc ttaaacagta ataaaaaagt tatgacaaga tgttgtcaca accttttttg
    34981 agtaaacggt gttcaaaaac ttgcatctgc ggtaataagt tcaaccaaac aaaaaacatg
    35041 aaaatctgtt tttgtttggc tgttcttact acttgatgca gggcagcttt ttcactacct
    35101 ctttttaata accataaaat cctattcctt catatctcca gcccatcggt gttagccaat
    35161 ctctttcctt agcactcaag gtatagtgat gggtaccagt tttttcattt ggattaaata
    35221 gtcgataaat tggcttggaa ccaccagaat aaaaacttag accttcataa cgccaaccga
    35281 tttttgttaa agagttgatc tcagattgtg ccattgtgta atgatggtct ccactgtaag
    35341 gattgtataa acggtagaca ggttgtccgc tgtttggtgc ttgccaagca atcccttcat
    35401 attcccagcc tacagttggc agataatttt tttctccggc attcaatgta tataaatgtt
    35461 ctcctgtatg acggttatac agacgataca tcggagaagt tgaagttggt gctgtatcat
    35521 actgtgtcag attataggtt tcgattacat tgttcaatgt agaaccataa ttaggcgcag
    35581 tggcataacg acctgttagc caagccgtag catctttgta agagttcgta ttgcttttcc
    35641 atgcaccgga atagtagtaa acgcctggct gaaaagaagt cgtcttcaat accgttacgt
    35701 tatcttgcaa tgattctttg tacgaaggat attttctaaa agcggcattc accgtaatcc
    35761 attgcccatt cacatattcc gaagtaggca tcgtaacaga ttggccatta tagcttcctt
    35821 taatcccaaa tagattgtaa ttaggggcag aagccaaagt actgttcccc cagccacttt
    35881 caacgatggc ttgagcaatc atgactgatg catataagtc attggcactt gccaaacttt
    35941 gtgcagttga ggcaatacta tcaatgaacg cttgttgaga attagcagat gagcgagcat
    36001 aaaaagcata gccagtatag ttttcgaatt cagacaaatc tgcttgatgg gtatgtgtca
    36061 atgtttgccc atctgtcaat tggttcgtga tttcttctgc attctctgga ttgttctgag
    36121 cttctgctga tgtactgtct tcggtttgtg tttgttctgt tgtttcagtt gttggttctt
    36181 cggttgaaat tgtattttct gagacagtag ctgactgcaa agtactttct gtatcgtttt
    36241 caattcctgt ttctgaagaa ctcgatgtct gagttgcgac agtttctgtc gattcttcag
    36301 ctagcactgt tgtgttggca actgctagat ttcctaaaag aataacgcta aataataaat
    36361 tctttgcttt catctttttt ctcctttgaa ttatgaaact tttcctaaat aacagtgtaa
    36421 ctaaaaaagt aaaagcaagt caatgtccat atttaaataa catttgtttc tttttaataa
    36481 atttgctttt tctgcataaa aagacgtaaa tgaagaatat tcttctccat tcacgcctta
    36541 aattgatggt cgtttgaata actatttaac aagtaattct ctctggatat tatccgttat
    36601 ggattatact tcctgtgtcg tcggacggat aatcatgttg ctgacagaca tccggtctgg
    36661 cgtttctatc gcaaatgcta cagcagaagc tatatcttca gatgttaatc cccattcttt
    36721 ttgcgcttca tgaagttcaa gagcagcctt tttgtcgctg atggtttgat ataattcagt
    36781 ttgaacagct cctggagaga tgatcgtaga tttgatgtta ttttgccgtt gctcttgacg
    36841 taatccttcc atgattgcac ggacagcaaa ttttgttccg caataaacgg cggaatcagg
    36901 ataaacaaca tgtcccgcca cagaatcagt tgaaatgata tgcccagatt tttgtttttc
    36961 catgattgga agtacggcgg aaattccatt taacacaccc atgatgttga tatcaagcat
    37021 attttgccat tcatcacggt gaccttcaac taatggagcg gttggcatga ttcctgcatt
    37081 attgtacaat acatcgattc gaccgaactt ttccatcgct aaatcgataa ctgcttggac
    37141 ttgtgcaaaa tctcttacat ctgctgtttg aatatatagc tcagcttctg gtaaagattc
    37201 ttttattgct tttaggcgat cttctcggcg tgcagctata actagtttag cccctttttc
    37261 tgctaatagc ttcgttgttg cttcaccgat tccgcttgaa gcgcccatga tgacaatcac
    37321 tttttctgat aaatcattca ttctattctc ctcctgtttt gaaaaatctc ttgcttatac
    37381 tcactatctc ttatttcatc ttactgttca aataatccgt tcgacaggtt tgacgatttt
    37441 ggcagggatt cctgcaacga tggtattgtc tgggacatct ttcgtaacag ttgcattcgc
    37501 tgcaacaatg gcattctcgc caatcgttac gcctggcaga atagttgctc ctgctccgat
    37561 ccaagcattt ttcttgatgt ggattggctt tactgacaag cctcttcgtt cttttggatc
    37621 ggttagatgg ttcaccgtga tgaggttgac acgaggacca atcaatactg aatcttctaa
    37681 acagatgcca cctaagtcaa cgaacatcac ttgacgattg ataaagacat ttttccctat
    37741 actaatatgt ttgccgaaat ctgtataaaa aggcagcaga acagtcaccg tatcatctac
    37801 tttgtcacta atgatatttt ctagccgttt gcggatatct gctggttcat ggtacccagt
    37861 attcagcgct gctaaaggct cttcgttttc cgcttggatc tgatggatca cttcaaagag
    37921 cggatccgtt actcgaatct ctttattgac gatttgtttt tcgatgaggc ttgtcatttt
    37981 gctccctcct tttatcctta ctttataacg gacggcttat gatgtctaat acttataaaa
    38041 catacttaac tattcctttt aactatactt agtggtatgc ttttttaaaa acaggaggga
    38101 aaaaatggat attcgtatac tgacgtattt tttagcagta gctagagaaa aaaatatcag
    38161 ccgagcagca aaatctctgc atatcacgca acctactctc tcaaaacaac tgaaagattt
    38221 agaagaagaa cttggtgtga cacttttcac tcgaggctcc cgagagatac agttgacgga
    38281 ttctggtcga tatctttatt caaaaggaaa agaaatcttg tcgttggtag aaaaaacggc
    38341 gaacaattta acaaatgacg caatcatctc tggagaaatc tacattggcg gaggcgaaac
    38401 taaagcaatc tctttcattt ctgatagcct tcattcgttg atcaaggcgc atcctgatat
    38461 ccagtttcat ttatatagcg ggaatgcaga tgatatcata gacaaactgg atagcggatt
    38521 acttgatttt ggactcgtaa tcgaaccgac aaacaaacaa aaataccagt catttcgact
    38581 gccagcccaa gatcgttggg gacttttgat acatcggaat catcctttag caaagcaaac
    38641 agtcatccat ccgcaggaac tgaaaactgt tcctttgatc atttcccggc aaacgtcagt
    38701 ggacagtcaa ctagctgaat ggctaggttt cagcatagat cagttatcga tcgtcggaac
    38761 atataactta ttgtacaatg cttctttgat ggtcgataac gggacttttg gtgcattagc
    38821 tctagatggg atcatcaata cttatgggac ggaaaaagta ttcatcccac tcgatccgcc
    38881 attacaagct tcactgaatc tcatttggaa aaaagaccag cctctttctc ctgctgctcg
    38941 taaattttta gatgtcctaa cggagaaata cagttaagta gagttttttt ccttcgcttt
    39001 cagcttttta acaacgaata agttaagcaa aagatccaat gattaaataa cataagtttc
    39061 ttaatttgtt ccttcctttt atttctgata attccagtaa attactataa ttataaaaaa
    39121 agaattgaca aaacataact gtctatataa aattgattac gttaacaata attcgtttca
    39181 tgtgactaga tcatactagg catgaaaaat ctctgaggaa catggtttcc ctaggaggtt
    39241 tttcgtgtct atttttgttt ggccgcaatc aactggtcta gcaaacgtga aaaccaaatc
    39301 ttcaaaaagg gtgcgcgcat gaaaatcaaa aagatcttta atcaaaatgc agtattggta
    39361 gatgacggtg gacaggaaaa agtagctatt ggaaaaggga ttggatttga cagaaaaaga
    39421 aatgatttga tttttgatcg tgatgtcgaa cggatatttg tcatggaacc agaaggtcaa
    39481 atcaagttgc aaaatttact taatcagatt gatgaacgct ttttatttgc agcagaaacg
    39541 atcattgatc atgcggaaac tgttttgatg gaaaagttga atgaacatgt attgatcgct
    39601 ttgaccgacc acattgcttt ttctgcggag aacattaata acggtatcgt catccgcaac
    39661 aaacttttaa gagaaatcga agtgctttac agtgatgaat tcagtattgc tcaatgggca
    39721 gtagagtatc tgacaaaaga gcttgggatt ccatatactt atgacgaagc aggatatatc
    39781 gcaatccata ttcacagcgg acgaagcggt cggaacaata accatcgcag tatccgtgaa
    39841 gtgacgattg tatctgacat catccattta gtggaatcag aattggaaac agatatccat
    39901 tcagaaccat tttcactgaa ttattcaaga ctggtgaatc atttacgact gcttctacaa
    39961 cgaacccatg cgcaacaata tgctgtttta gacacggaaa tcgtagaaat ggtcaaacga
    40021 aaatacccag aaagctataa gatttctaaa aaaatccgtg tgttgctgac caaagaatac
    40081 cagctcgcga tcacgaaaga agaactgggt tacttggcca tccatatcga gcgtttaaga
    40141 agcgcaatcg tacaaacaaa tgttaacaat catgaggagg aaaaaaattg aaagcatatc
    40201 tacaacgaat gggacgttcc cttcaacttc cagttgcggt cttacctgct gctgctttac
    40261 tagtagggat cggaaactgg tgggcatctt atagtaacga cattgtcgct cattttttac
    40321 aagctggcgg taacgcagtt ttaggtcaat tacctttgct gtttgccgtt gggttagcac
    40381 tagggatgtc agtggacaaa tcaggtgctg ctgccttggc aggtttggtc gcatttgaag
    40441 ttccagtaaa tgttttaaaa actgattcag tagctacttt attgaatatc aaaccagaat
    40501 cagttgatcc atcttttgct cagatcagta acgtatttat tggtattatt gccggattga
    40561 ttgcggcagc tctttataat cgtttccacg aagcaaaatt accgatggct ttatctttct
    40621 ttagcggcaa gcgtctagtg ccaatcttgg ctgcctttgt catgttgctg atcagtgcag
    40681 tgctactgct cgtatggcct ccggtttact ctgctttagt ttcttttgct aaattcattt
    40741 tgagtttagg ctggggtggt gcaggtcttt atggcttctt caaccgttta ctgatcccta
    40801 caggattgca tcaagcaatg aactccgtgt tctggtttga cgtagcagga atcaatgata
    40861 ttggtaaatt tttggcttct gaaggaacaa aaggcgtaac aggaatgtat caagctggat
    40921 ttttcccaat catgatgttc gggcttccag caggagcata tgctatttac agaaatgcac
    40981 gtccagaaaa caaagcaaaa acagcttcct tgatgttagc agcagctttt gcttcattct
    41041 ttactggtgt aacagaacca cttgaattct catttatgtt cgttgcttgg ccattatatg
    41101 tcttgcatgc aattttcact gggatttctt tagcagtgag tgcattcttc cattggacag
    41161 caggatttgc ctttagtgcc ggatttgtcg attacttcct atctttgaaa aatccgattg
    41221 cgaatcagcc attgatgttg atcgtccaag gattagtaac agcagtgatt tactactttg
    41281 gctttaactt tgctatcaaa aaattccatc tgatgactcc tggccgcgag gaagcagatc
    41341 tttcagatga cgatactgca acgactaatt cagacaataa atatgcggca caagcaagca
    41401 aaatctacgc tgcattaggc ggagccgaca atgtaacttc gatcgataac tgtactacac
    41461 gccttcgttt gcaagtcaaa gatacaggcc tgatcgatca aaataaaatc aaagccactg
    41521 gcgtacctgg tatgaagatc atcgacggaa aaaatgcgca agtcatcgta ggtacagaag
    41581 tacaattcgt tgcagatgag atggcaaaac tacatggtgg tgctgcaaca cgacctgcac
    41641 aaacaaacac cgtggttaaa acagaaacat ttgccggtgg aacgatcgaa caagatattt
    41701 acgctatcgc aaatgggaaa ctgattccaa tcactgaagt ttcagatgat gtattcgcgg
    41761 aaaaaatgat gggtgacggc tatgctgtat taccagaaaa tggagaaatc ttctctccaa
    41821 tcgctggtac gatcacaaac attttcccaa cgaaacatgc tgtcggaatc caaacagatg
    41881 ctggtatcga agtcttgctt catatgggca tcaacactgt tgacttaaaa ggcgaaccat
    41941 tcactttata tgtaaaagaa ggtcaaaaag tcgcaagagg tcaattgatt gctttagttg
    42001 atcttgccgc tattcaatct gcaggcaaaa acacggatat ggtcgttgta ttcacaaatg
    42061 gcgacaaagt ccaaagctta gaaatcgaac ccgcacgtga tgtaaaagca aatgataaaa
    42121 tcggctctgt ttccaacaaa gcctgagcca tcgtcttccc cagacttttt tatcgataaa
    42181 caaacaggag ctgagacaaa aatcttgtct cagctccttt ttccgaataa aaacggcgtt
    42241 ctcctgccaa ctccgcggta ataagtccag ccaaacgtaa catatgaaga gctatttttt
    42301 atttgactct tcttattact tgatgcagaa cagctttttt acaacctctg ataaacggtg
    42361 ttcaaaaacc agtttatcgg tatgaagtca aatttatgca aaaatgtgga aagccatttt
    42421 cacaaaattt ttcttcatac tcaaaactaa tcggcttttt cacaacctct ttatacatta
    42481 ttcctgatcc aatatttctt ttaatgctgc ttcaagagaa gttaatggtt ttcccaatac
    42541 tttttctaaa tcatcagagg aaatagctaa ctgatccttt ttaatatcat gttggatcgc
    42601 taaaaacatg tcagctgctt cttgagggaa tccggcttct tttagatttt cgacaaacgc
    42661 tgcatcactt gcttcaataa tttctaactt tttacctgtc gcacgctcta atgccttggc
    42721 taattcttca tagtcagtta gattgccgga taattctaag atttctggag agtcactttc
    42781 tactagtact tttgctgcag cttctgcata ttcacgtttc aatgcccaac ctgtttttcc
    42841 atttcctgca gcataaacga attgaccagt ttttaaagcc tgcccaatga taggtaattc
    42901 attctctaca taccaattat tccgtaaaaa tgtatgggaa atacctgaat ccttaatcat
    42961 tttttcagtg aattggtgat ctggcgctaa tacactgatc gagtgatcgg catctgcaaa
    43021 acttgtataa gcgatataag ataccccagc tgcttgtgct gccttcacta cattcttatg
    43081 ttcggcttga cgatttcctg gaacgccaga gacaaaaagc aatcgatcta ttcctttcaa
    43141 cgcttctttc attgagtcta aatcatcata atcaccaata cgtatattaa atccagcttc
    43201 tttcagtgct tctccttttt cttcactacg tactagtaca tagatttcat tttgtggaac
    43261 catctttttt aagaattcca gcgcataacc accaaaacct ccagttgctc ctgttactaa
    43321 ataatgcata tttgccactc cctattcttt ttatttgtta agatttacag ctgtacattt
    43381 ataaattaca cctttatcat aaagagtgtg gtaagataag tcaagcaaaa aaaatgaaag
    43441 gatgacaact gatgaagtat tctctgcaat ttagtgatgc aatccatgta cttgcctata
    43501 ttgaaatgtt tcaagatacc cgatcacttt ctagtgagat gattgccagt agtatcgaaa
    43561 caaatgcagc aaatgtacgt aaaatcatga gtcagctcaa aaaatccggt ttgatcttta
    43621 caaaaaccgg aaaaccgcag ccttcccttg ccaaaagtcc agatcagatt tctttactag
    43681 atgtctatcg cagcatcgaa ggtaatacac agcttatcca agtcgatcca aaaacaaatc
    43741 caaattgcgt cgttggtgcg aatatccaag aagctttaga agaaagctac gctcggctgc
    43801 agaaaaaggc agaagaagaa atggctaaga tcacacttga tagtctgata cataaaatag
    43861 ccgttttgga aaaagaaaaa agaccagaaa acacaggcct cgtagagaga tttttataat
    43921 tctctctact gtgcctgcat ttttctgatc ggaatcgtct acataaattc ttttattctc
    43981 cgggggacaa aagccttgtc atgccccttt ttcctctcta atgctcaggt caaagcgctg
    44041 aagtcatgac gtcttgataa acggtgttca agtagctgac cctcggcatt agttctgaaa
    44101 aatgcaaaat atggaaagct atttcccatt tttcctctct aatgctcagg tcaaagcgct
    44161 actttcacga cctctatatt atcatggttt attgactacc ggtatccaga tttctcccat
    44221 atatttcccg ttggtaactc ccatttcgac tacagcattt ggacccccaa cataggcgta
    44281 atcttctact tctcttaaaa catgcccaaa ggccgtattc gtcaaatcat ttcttaattc
    44341 ttcaggcgat tctgctaaag cgattacaac aagatacgtt ccttttggga atgggatgat
    44401 tcgggtagtg tttggaacat cctgactcgt ttgaacaccg acatagtaca tcattttccc
    44461 gttatagggt tcgttgactg taaagaaata atgattttca gcagcttgtt tcaatgcatc
    44521 tattgttcca ttaccgataa ctttttgctc aaatgcttgt ttctctgcag ccaaaccgac
    44581 aaaatccgta ttgctactct tcaattccac accaataccc gtcaccgtaa aatctggttt
    44641 ttcttcgatc ctatactttg ccatttctcc caccctttct attatattaa gttcatctta
    44701 cataaaatta aagggaaatg acacgattac gctctttttt ctagggatat ttttattttt
    44761 taactatttt tttgctttca ctgcaatcca aatctcactg taaacattac tgaaatcctc
    44821 tccccatttt gtaaaagaga tctcgggagc ttcgactaac tcataatctg aagaaggaag
    44881 ccattctgca tagattttcc cccaagtatc ttgtaaagtt tggggaaatg gccctttatt
    44941 aggaaaaatg gcccaagtgt gttcttctat agcaagcatt tctaaatcag cgtacggatt
    45001 ttcttttgtc gtagcaaagc caatcatgtg catcaaactg cccttttctt ccaagcgggc
    45061 tccatcaaat ccataagaaa cgttgattac ttgatttgga tataaatctc ctaacttatg
    45121 catctcttcc ctttgagtct ccgtgatcgt ctgcgctaac ttttgtattt cttgattgac
    45181 tccttcaaat ttgacaggaa cttttttcgc cacacctgcc acataaaact gttctttctt
    45241 ttcgatacga aattccatcg attttcctcc tctgacatca ataaaaaatg tcaagcgtgg
    45301 aaaagatttt tgtatgccgt atctgctgat ttctgaaggc agatatcctg accaatcacg
    45361 aaaagctctt gaaaaacctt cgactgtttc atatccatat ctaaacgccg tttctgtcac
    45421 tcttgatcca tctaggagat cgcgattagc caaagccaat ctacggtttt tgatatagtc
    45481 cattagcgac atccctgcaa taaacgtaaa gatgcgtttt aattgatatt cagagattcc
    45541 tgtattttcg gctattattt tataggaaag tccttctgtc aaatgttctt cgatgtaatc
    45601 gatcaattgg ttcagatctt taagcatatt tatcttcctc cttgcttgcc tttttatcat
    45661 attatcaaag aaagtcaatt acttgatgaa tcaggaacca atttagtcag atgtagaaaa
    45721 tgttgtatgg atctaaagaa aattctcaag cagcgtttcg taacggataa ttggtattat
    45781 ctgcctatct atgttttacc tttagaaaaa taaattttgg ggaagacaca ttctaagtaa
    45841 acaaagcggt aaaaatatct tttattccct tagttaatcc gcttactacc tactgtacaa
    45901 agcaaattgt tgttcaaact gctgatcaga acgctgcttg gatttttctt tgcgatacat
    45961 catacctctt ttatcaattg gaacaattat ttatttctaa caaaatcact tcatttttgt
    46021 cagataaata aa
//
LOCUS       DACEEZ010000014        42007 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20313_2017
            SAMN12639966-rid9879223.denovo.014, whole genome shotgun sequence.
ACCESSION   DACEEZ010000014 DACEEZ010000000
VERSION     DACEEZ010000014.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639966
            Sequence Read Archive: SRR10023158
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 42007)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 42007)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741683.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741683.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:44:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,016
            CDSs (total)                      :: 2,962
            Genes (coding)                    :: 2,889
            CDSs (with protein)               :: 2,889
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 36 of 73
            Pseudo Genes (incomplete)         :: 29 of 73
            Pseudo Genes (internal stop)      :: 18 of 73
            Pseudo Genes (multiple problems)  :: 8 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..42007
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639966-rid9879223.denovo.014"
                     /isolate="E20313_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            122..1558
                     /locus_tag="IWO89_000841"
     CDS             122..1558
                     /locus_tag="IWO89_000841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HAQ0455614.1"
                     /translation="MEHKELTSFPKDFLWGSASAAYQVEGAWQEDGKGESVWDRFVRI
                     PGKTFKGTNGDLAVDHYHRYKEDIALMKEQGLKAYRFSVAWTRIFPNGRGEVNQAGLA
                     FYERLIDELIENDIEPMLTLYHWDLPQALQDEYNGWESRQIIEDFTHYAETLFEAFRG
                     KVHYWISLNEQNIFTSLGYLLAAHPPGVTDPKRMYEVNHIANLANASVINKFHELEMP
                     GKIGPSFAYTPNYPIDSNPENVLAAENAEDLMANYWMDVYMWGKYPIAAMRFLEEKGW
                     APTIEAGDAELLESAKPDFLGINYYQTATNAFNPLNGVGAGKMNTTGKKGSSEETGVP
                     GMYKKVENPFVERTNWDWEIDPEGLRIGLRRITSRYRMPVLITENGLGEYDKLTEDKQ
                     IHDDYRINYLQSHVKAIKEAISDGAEVLGYCTWSYTDLLSWLNGYQKRYGFVYVDQDE
                     TQEGSLERYKKDSYYWYQKLIEENGENL"
     gene            1747..4194
                     /locus_tag="IWO89_000842"
     CDS             1747..4194
                     /locus_tag="IWO89_000842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HAQ0455615.1"
                     /translation="MAQKKRPTKKRKKTKKTKKQKQQQEQMLTIGVGFALILFAVFGF
                     LKLGFLGILIANGFRIIAGNTYQILCLLLAVLGFWIVIKNTEFSIGKNRRWFGGILFY
                     FGILLLLHAHLFGKLHTGEPNIMGTTWDLLASDIKQSQVDNNVGGGMIGAILYHFTYF
                     LIAQPGSYLAAVLLLAGGAFLFSNLEGYQLLNGIQSIGERVQELLEGDPAKQARKQAA
                     KEERMKQRAEAKEARRLAAKEAAEKEAAEYEKKKAVSQQRENTADEWQEPENTEPEQL
                     SFVPIDSFQENIHPANLEKPVPDTPKQTNTAEGFSDELPEDDGTSLEFEIEAEQENQD
                     YELPTVDLLDSIPTVDQSDEYKKIEKNIGVLEQTFQSFGVDAKVVKASLGPSVTKFEV
                     QPAVGVKVSKIVNLTDDIALALAAKDVRMEAPIPGKSLIGIEVPNGKISMVSFREIIE
                     AQPNHPDKLLEVPLGRDVSGRVQTADLSKMPHLLVAGSTGSGKSVAINGIITSILMRA
                     KPHEVKLMMIDPKMVELNMYNGIPHLLTPVVTNPRKAAQALQKVVQEMEERYEKFAAT
                     GVRNISGYNEFVQQKNLENGTKHPTLPFIVVIVDELADLMMVASNEVEDAIIRLAQMA
                     RAAGIHMILATQRPSVDVITGIIKANVPSRMAFAVSSGTDSRTIIDSNGAEKLLGRGD
                     MLFLPMGENKPIRVQGAFISDHEVERVVQFVTDQQEAHYEEKMMPTDEVETTGAPEQP
                     QDELFEEAKALVVEMQTASISLLQRRFRIGYNRAARLVDELEAHGVVGPSEGSKPRKV
                     FIEQEETSETLPDDNDM"
     gene            4424..5491
                     /gene="rlmN"
                     /locus_tag="IWO89_000843"
     CDS             4424..5491
                     /gene="rlmN"
                     /locus_tag="IWO89_000843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2503)-C(2))-methyltransferase
                     RlmN"
                     /protein_id="HAQ0455616.1"
                     /translation="MEKQSIYGLTNEELINWFIENGEKKFRAAQVWEWLYQKRVSNFT
                     EMTNLSKQLIEKLSEHFIINPLKQMVVQEASDGTVKYLFELPDKNMIETVLMRQEYGL
                     SVCVTTQVGCNIGCTFCASGLLKKNRDLTAGEIVAQIMMVQHYFDERNLGERVSHVVV
                     MGIGEPFDNYDNVMDFLHIINDAKGLAIGARHITVSTSGLAHKIKEFANNGLQVNLAI
                     SLHAPNNEVRTSIMRINRSFPIEKLMEAVDEYLEKTNRRITFEYIMLNQVNDRPEHAQ
                     QLADLLKDKKKLAYVNLIPYNPVSEHDQYSRSPKADVLRFYDVLKKNGVNCVIRKEHG
                     TDIDAACGQLRSKQMKKTVAK"
     gene            complement(5684..6787)
                     /locus_tag="IWO89_000844"
     CDS             complement(5684..6787)
                     /locus_tag="IWO89_000844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase family
                     protein"
                     /protein_id="HAQ0455617.1"
                     /translation="MSQSVFLPNYTIGTDAYEKANGIVKEYGTKVVLIGGKTALSKAE
                     NKIKVALTQEIEVVASLWYGGNSTYGNIEQLIQHESVQKADVLFAVGGGRACDTVKVI
                     GEKLAKPVFTFPTIGSNCSPVTAVCVIYNEEGVMDGLFFPSKPPVHTFIDTAIIAEAP
                     DKYLWAGIGDALSKEVESTFSARGDDLGYTDRLGIQIGKMCIDRLLTYGTQALKDSAD
                     DRPSYAIEQVVSDIIVTTGLVSVLVNNNYNSAMAHSFYYGTTVLEKMEGYLHGASVSY
                     GVLTLLMMDQQFDLFDKIYVFMKKNQLPVSLTDMNLNVTTDLEAILDKAMATNDIKHV
                     PYEITREKFKQAILDVETYHATQESQQGYKKIS"
     gene            7340..7888
                     /gene="aac(6')"
                     /locus_tag="IWO89_000845"
     CDS             7340..7888
                     /gene="aac(6')"
                     /locus_tag="IWO89_000845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside N-acetyltransferase AAC(6')-Ii"
                     /protein_id="HAQ0455618.1"
                     /translation="MIISEFDRNNPVLKDQLSDLLRLTWPEEYGDSSAEEVEEMMNPE
                     RIAVAAVDQDELVGFIGAIPQYGITGWELHPLVVESSRRKNQIGTRLVNYLEKEVASR
                     GGITIYLGTDDLDHGTTLSQTDLYEHTFDKVASIQNLREHPYEFYEKLGYKIVGVLPN
                     ANGWDKPDIWMAKTIIPRPNSQ"
     gene            8434..10095
                     /locus_tag="IWO89_000846"
     CDS             8434..10095
                     /locus_tag="IWO89_000846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HAQ0455619.1"
                     /translation="MKKKFTFGLVAVCGLVLAGCYGGSSATSSSSAKSGSADGGGVFN
                     LVVPQEMPTADLSVATDTISFTALNNVYEGIYRLDEDSKPQPAGASELAEVSEDGLTY
                     KIKLREDAKWSNGDPVTAADYVYGWQRTVDPATASEYAYLYAPVENAEDITAGKKDKS
                     ELGIKAVGDYELEIKLTKQTPYFQYLLAFPSFFPQKQSVVEENGDAYASASDKAVYNG
                     PFTLEDFDGPGTDTEWTYKKNDHYWDKDTVKLSEIKVSVVKESSTALNLFKDGQADDV
                     ILSGELAQQNANDPAYTSVKEARTSYIEFNQREKNSPFNNVNLRKAISYSIDREALVK
                     QVLGDGSVVSTGLIPSDMVKSPTTNEDFAKEAGKLVSYDKDKAKEYWEKAKKELGVDS
                     LEFDLMASDDDSSKKVIEYVQNSIQENLDGVTVKPTPVPFSVRLDRSTSGDFDAVLGG
                     WAADYADPSSFTDLFVTGNSYNRGRWSNAEYDEAVKAASSTDAGDEQARWDDFQKAIK
                     IISDDMGVVPVYQKAEGHLVNEKIKGIVHHAAGASWDYKWTYVEE"
     gene            complement(10165..10512)
                     /locus_tag="IWO89_000847"
     CDS             complement(10165..10512)
                     /locus_tag="IWO89_000847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3899 domain-containing protein"
                     /protein_id="HAQ0455620.1"
                     /translation="MKKKSIPYAVAFLLILVILIKNLINHSFTLIQLSNDLFLWSLPF
                     LIIGGFLWVFSSGFFDHFQRSVHLARTRNRKKKPEFSSLSSASYGMYSFWLIIAGILI
                     ALSAIFMLFSLLG"
     gene            10596..11537
                     /locus_tag="IWO89_000848"
     CDS             10596..11537
                     /locus_tag="IWO89_000848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016184893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAQ0455621.1"
                     /translation="MNSYIKYVLKRVFFMIITLWLIATITFFLMQLLPGTPYTNQERL
                     SPETIEMLNKQVGLDKPVIVQYGIYLSNLLQGDFGISFQFKNQPVAHLLAGRIGPSLQ
                     LGLQAIIFGTVLGTILGTISAMKQNTWADTSSTLVAILGRSIPNFVFAVLLQYIFAIK
                     LQVLPIAKWDGFVYTILPTIALAMSPLADSARFIRTEMVEVLHSDYVELAKAKGLSRW
                     EIAFKHGLRNSLIPLMTLLGPLAVALMTGSLVVENIFAIPGIGEQFVKSITTNDYPTI
                     MAVTILYSFMLIFVILIVDLLYGLVDPRIRVSEGSRG"
     gene            11538..12581
                     /locus_tag="IWO89_000849"
     CDS             11538..12581
                     /locus_tag="IWO89_000849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAQ0455622.1"
                     /translation="MEMIPKRYETVADIPADEFLPLQKNTEEEREQITAPSLNFIQDS
                     WRRLKKNKAAVISMVLLLVIILISVITIFVSPHDPTAQNVAYINLPPRIPGINIDGLN
                     GKAMVGGELVDKYAQANVPSNVNFFLGTDGLGRDVLSRLFMGMRISLLIAFIAALLDV
                     TIGVAYGLVSGLLGGRVDNVMQRFLEILSGIPNLVVMILMLVVFEPGIFSIVAAMAIT
                     NWIPMARIVRAQTMKLKDQEYVLAGLTLGESRMKIAFKHILPNISSVIIIQMMFSIPS
                     AIFFEAFLSFIGLGLTPPSASLGTMLSEGYKTFLYLPYLLWIPAVTLSVIMIGFNLLA
                     DGLRDAFDPKMKE"
     gene            12587..13657
                     /locus_tag="IWO89_000850"
     CDS             12587..13657
                     /locus_tag="IWO89_000850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ0455623.1"
                     /translation="MTKILEVKDLEISFDTYAGKVRAIRGVNFHLNKGETLAIVGESG
                     SGKSVTTRSIMRLLSSNANIDAGQILFKGNDIVGKSEREMQKIRGKEIAMIFQDPMTS
                     LDPTMPIGKQVAESLRKHNKVSKKEGQKAALELLKLVGIPEAEKRINSYPHQFSGGQR
                     QRIVIAIALVCYPEILIADEPTTALDVTIQAQILELLKEIQTKIDTSIIFITHDLGVV
                     ANVADRVAVMYGGRIVEVGTSEEIFYNPQHPYTWGLLGSMPTLDSANDRLYAIPGSPP
                     DLLNPPKGDAFYPRNEFAMKIDAEQEPPFFELSKTHKAATWLLAPQAPKVTPPEEIQR
                     RWAIFKEKQKHTYGQGGIADAK"
     gene            13647..14600
                     /locus_tag="IWO89_000851"
     CDS             13647..14600
                     /locus_tag="IWO89_000851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAQ0455624.1"
                     /translation="MPNKLLEVKGLKQYFNVGKKNEVHAVNDISFHIYEGETFGLVGE
                     SGSGKSTTGRTIIRLNEPTDGEILFDGQDITKIKDKKGLTKFRHDVQMIFQDPYASLN
                     PRMKVRDIIAEGIDVNGLAKSSEERAKKVDDLLRTVGLNPSHGTRYPHEFSGGQRQRI
                     GIARALAVKPRFIICDEPISALDVSIQAQVVNLLQDLQKEHQLTYLFIAHDLSMVKHI
                     SDRIGVMHNGLLLEMGTSDEIYNHGVHPYTESLLSAIPLPDPDHERKRRRIKYQPEPD
                     DGQVRQLREIAPEHFVYATEQEVPYYAKKLKRQKESLLVAN"
     gene            14612..15622
                     /locus_tag="IWO89_000852"
     CDS             14612..15622
                     /locus_tag="IWO89_000852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HAQ0455625.1"
                     /translation="MLKAFKFRMYPTEEQKQQLIRTFGCARFTYNHLLKKRQKSWQQT
                     GVANFSLTPATLKKEYPFLKEVDSLALANAQLNLDRAFRNYFKGRASFPKLKTKKSMW
                     QSYTTNNQTRTIYLENGYLKLPKQKELIKINQHRPVEGSIRSATISARYNEEFYVALL
                     CDVSPVKKESLAKWIGIAYHPKTLIQTSRPLEVTLPKFHQTEEKLQHAQRKLNVKVRS
                     AHHRKIRLDQASNYQKQKRKVMDLYLKQKNQREDYLEQLSGKLVKQYDYLFVESFPKE
                     EAHADFSIHDWHKLITKLRYKSQWYNKKFLLINTDGAEESNSVRKSQVVEKMGRHSVI
                     KG"
     gene            complement(15907..16728)
                     /locus_tag="IWO89_000853"
     CDS             complement(15907..16728)
                     /locus_tag="IWO89_000853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HAQ0455626.1"
                     /translation="MNRFASKEEEKLYYEHKASEEEFLNWYQKQERPEYDKPSLTVDI
                     VLMCYNKEEDQLKVLLIQRKGHPYRNSWALPGGFVQKDESTGESVLRETKEETGVVIS
                     KENIEQLHTFSTPNRDPRGWVVTVSYLAFIGEEPLIAGDDAKEVRWFTLERHSNKIHL
                     SSGEVAISLDLVTGESSGKDTLAFDHSQIILKAFNRVVNKMEHEPQVLQVLGKDFTIT
                     EARKVFAKFLGIDYKTIDHSNFKKAMLQYFDEIGERPVGIGRPSKIYQLKPGHHE"
     gene            complement(16825..17103)
                     /locus_tag="IWO89_000854"
     CDS             complement(16825..17103)
                     /locus_tag="IWO89_000854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0455627.1"
                     /translation="MQKKHLFFTLSIAFLSLAHLIFSYFYIRMYGYFNLHGHLNSFMT
                     AAWILRFIIDVYIVICGFFAIREERYKVLPFYLLFFLFNLILPFIFHI"
     gene            17371..19380
                     /gene="metG"
                     /locus_tag="IWO89_000855"
     CDS             17371..19380
                     /gene="metG"
                     /locus_tag="IWO89_000855"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HAQ0455628.1"
                     /translation="MAEKETFYITTPIYYPSGKLHIGNSYTTIACDAIARYKRLMGFD
                     VFYLTGVDEHGQKIETKAEELGVKPQEYVDKMAADVKKLWKTLDISYDKFIRTTDDYH
                     KAAVQKIFDRLLEQGDIYLGEYEGWYSVSDEEFFTETQLAEVYKDDEGNVIGGKAPSG
                     HEVELVKEESYFFRMSKYADRLLEYYEEHPEFIQPESRKNEMINNFIKPGLEDLAVSR
                     TTFTWGVPVKSDPKHVVYVWIDALSNYITALGYGSEDESLFEKYWPADVQMVGKEIVR
                     FHTIYWPIMLMALDLPLPKKVFGHGWLLMKDGKMSKSKGNVVYPEMLVERYGLDALRY
                     YLLRAVPFGSDGVFTPEDFVSRVNYDLANDLGNLLNRTIAMINKYCDGLVPDYASLVT
                     PFDSELSTTAANVVGRYHDAMEKMEFNTALAEIWVLISRANKYIDETEPWVLAKDEEK
                     KAELNSVMIHLAESLRIAAILLQPIMTETPEKIFNQLGLDSETMNLEGLHFGEFPSGT
                     KVVAKGTPIFPRLDMEEEVAFIQEKMSEGTQTNEDTVKWDPEETELVSTKEKQIKFDV
                     FEKVELKVAEVINCQKVEGADKLLQFRLDAGDSQDRQILSGIAEFYPDPSELIGKKVV
                     IVANLKPRKMRGQISQGMILSAEAPDGSLQVIEAPKSMPNGSEIA"
     gene            complement(19859..20446)
                     /locus_tag="IWO89_000856"
     CDS             complement(19859..20446)
                     /locus_tag="IWO89_000856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0455629.1"
                     /translation="MDFKRSYPKLADYLSNRVDFSRKEQQQSHWHWFTLILFGLMTVA
                     TLSQKLILLTLFIAIVALIKGPVMILWGMLYSFLVGLFPPIGILLSAIFFLLNIGTFT
                     KNWRMTLVGIYFYFYPFGVMALSEVMHWDNHWFIAGSLLLGLILLHVMLTKLYQHYGI
                     GRTIFWYVFSIPFALLTALLPSRLKTKIKGYHKIK"
     gene            20621..21943
                     /locus_tag="IWO89_000857"
     CDS             20621..21943
                     /locus_tag="IWO89_000857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAQ0455630.1"
                     /translation="MKGKLKREINLFGALATVMGTVIGAGVFFKTAAVTASTQSTSLT
                     LLAWLLGGFLTICAGLTVAELATAIPETGGAVKYIEAAYGKLPSFLLGWAQSLIYFPA
                     NIAALSIIFATQLTNLLQLSTDYLLLIAVITAVSVTGLNLLGTKVGASVQSVTLIVKL
                     IPIAVIVIWGLLTPGQGTVQLFPIEAGKDVTFVEGLSSALLATLFAYDGWLGVGAMAG
                     EMKRPEKDLPKAIILGLSFVTVVYLLINFVFLKTLPIDHLAGNLNAASEASDVIFGGI
                     GGKLVTIGILISVYGALNGYTLTGIRVPYAMALEDELPFSKQLTKLSKKFTVPYVPAV
                     FQLAVACIMMSLGSFDFLTDMLIFVMWLFSLLIFIGVFILRKKAPELPRPYKVPLYPI
                     VPIIAILGAIFILGMTMMTQTKLALIGIGVTLIGIPVYYQKKKKRSEE"
     gene            22098..22874
                     /locus_tag="IWO89_000858"
     CDS             22098..22874
                     /locus_tag="IWO89_000858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HAQ0455631.1"
                     /translation="MIFDSHTHLNAEQFNEDIPETIQRAQEMGVTKMAVVGFDTPTIE
                     KSLQLSHDYPNIYSIIGWHPTEAGSYTKEIENKLQEQLTLPKVVALGEIGLDYHWMED
                     PKEVQDKVFRRQIAIAKEMNLPISIHTREALEDTYQILKEEDVRDIGGIMHSFSGDYE
                     WAKRFLDLGMHISFSGVVTFKKALDVQEAAANVPIERLLVETDAPYLAPVPFRGKRNE
                     PGYTRYTVEKIAELRNLPTEEVAAQTWTNAHRLFGLSEHG"
     gene            22867..23436
                     /gene="rnmV"
                     /locus_tag="IWO89_000859"
     CDS             22867..23436
                     /gene="rnmV"
                     /locus_tag="IWO89_000859"
                     /EC_number="3.1.26.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease M5"
                     /protein_id="HAQ0455632.1"
                     /translation="MDKMKIQEIIVVEGKDDTRRLREVVDADTIETIGSAINEEILMQ
                     IEHAQETRGVIVFTDPDFSGEKIRKIIMEAVPQAKHAFLPRTQAKPKKKGSLGVEHAS
                     DAAILEALKKVVTPDISQTAVSEITRQDLLDYGLIAGAQAKEKREKLGDELRIGYTNG
                     KQLEKRLKMFRITPKELAQAMKIVEEQHE"
     gene            23429..24313
                     /gene="rsmA"
                     /locus_tag="IWO89_000860"
     CDS             23429..24313
                     /gene="rsmA"
                     /locus_tag="IWO89_000860"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HAQ0455633.1"
                     /translation="MSEYREIATPSRTKEILLKHGFSFKKSLGQNFLTEPNILRKIVE
                     TAQIDEHTNVIEVGPGIGALTEQLAKHAKQVVAFEIDDRLIPVLADTMQPYDNVTIVH
                     QDILKTDLSTAVRETFHEELPLKVVANLPYYITTPIMMHFLESDLVVDELVVMMQKEV
                     ADRISAEPGTKAYGSLSIAVQYYMEASLAFIVPKTVFVPQPNVDSAILKLTRRDIPAV
                     EVTDEKAFFRLTKAAFQQRRKTLWNNLQHSYGKDDQTKAWLAKSLETAGIDPKRRGET
                     LSLQEFAALSNAMSENMQ"
     gene            24764..25393
                     /locus_tag="IWO89_000861"
     CDS             24764..25393
                     /locus_tag="IWO89_000861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0455634.1"
                     /translation="MRKNSVRKLTISALLIGMGVIIPMVMPKIMIPPASFTLASHVPL
                     FIAMFFTPGVAVAVALGTTFGFFLTTPVIIALRALSHLVFALIGAWYLQKHPSIVLKN
                     GQFTFANWRFQGFNFVIGVIHSLAEMLVVSIFYFIGNMPETYYSQGYFYTVFILMGIG
                     GLIHSLVDYNIAYFIAGTLSKYFDIPVFTAAKKENRKESIQGYAKKTTY"
     gene            complement(25517..26626)
                     /gene="ugpC"
                     /locus_tag="IWO89_000862"
     CDS             complement(25517..26626)
                     /gene="ugpC"
                     /locus_tag="IWO89_000862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter
                     ATP-binding protein UgpC"
                     /protein_id="HAQ0455635.1"
                     /translation="MVEMALKNVYKKYDNAENYSVTDFNLSIKDREFIVFVGPSGCGK
                     STTLRMIAGLEDISEGELNIGGKVMNDVAPKDRDIAMVFQNYALYPHMTVFDNMAFGL
                     KLRKYDKADIKKRVDNAAEILGLTEYLKRKPAALSGGQRQRVALGRAIVRDAKVFLMD
                     EPLSNLDAKLRVAMRAEIAKLHQRLETTTIYVTHDQTEAMTMADRIVIMKDGFIQQIG
                     TPKEVYDTPVNMFVAGFIGSPAMNFFNVTLKNGVITDGHGLKLRIPEGKNKVLVEKGY
                     EGKEVIFGIRPEDIHSEQVALEASPEAVVKSEVVVSELLGAETMLYTKTGSTEFISKV
                     DARDFHKPGEIVDLAFNISKGHFFDKETEQVIKLP"
     gene            26835..27257
                     /gene="mgsA"
                     /locus_tag="IWO89_000863"
     CDS             26835..27257
                     /gene="mgsA"
                     /locus_tag="IWO89_000863"
                     /EC_number="4.2.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010763995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylglyoxal synthase"
                     /protein_id="HAQ0455636.1"
                     /translation="MKIALIAHDRKKPMMVKLATAYKTILEKHELFATGTTGQRITEA
                     TGLPIHRFKSGPLGGDQQIGAMISEDQLDLVIFLRDPLAAQPHEPDVTALIRLSDVYE
                     IPLATNIGTAEVLLRGLEAGFLDFRRIVHEIDKHPLSF"
     gene            27580..28116
                     /locus_tag="IWO89_000864"
     CDS             27580..28116
                     /locus_tag="IWO89_000864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="folate family ECF transporter S component"
                     /protein_id="HAQ0455637.1"
                     /translation="MKKHRLDARMIAIMGLLIALMVTLSRLVAIETPFIKISVTFIPQ
                     VIMGILFGPFWSGIGAVLADLVGMALFSKSAFFIGFTLNAFIEGAIYGFFFYRKEITW
                     KNAILATLSVTLIINLFLTPLWLALMYHVPLFSWVVWAPRLLKTVIWLPIQSIAIYYV
                     GRSIPYKKILRSLAIHAK"
     gene            complement(28156..29919)
                     /locus_tag="IWO89_000865"
     CDS             complement(28156..29919)
                     /locus_tag="IWO89_000865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ0455638.1"
                     /translation="MKVKKLSLQTLRRFIPYLTAYAKDIWLAVILGIINGIATVVMTL
                     QIGQSIDQMIGEGQVRFNRLFHLLILFGGIVLINVVSQWLIQVLSNRLAYLSVAKLRK
                     DAFAHLNRLPLRYYDQNSHGNIVSRFTNDMDNISIAISAAFNQLFSGVAIIILSFIFM
                     MRLSPLLTLVVLCSTPIIFLVSWIVARASQTNFDRQQRIIGDISGFVTERIGNQKIVK
                     AFQQEEASQQQFELLNADLYEKGQRAQFSSSLTNPSSRFVDHLAYLSIGLVGGLLALK
                     SGSSITVGVISSFTIYSSQFSKPFIELSGITTQLQTAFSGLERAFDLIDQPEEKPDLP
                     DAYALEPKNIKGKVSFDHVSFSYDPKQRLIEDFNFTAYPGQTIAIVGKTGAGKSTLVN
                     LLMRFYDTTKGTITVDDHDIRSIQRDTLRKSFGMVLQDTWLFDSTLRENLRFGNPEAS
                     DAEINDALKASYMYEFVERLPEKLDTKIGGQGLKISDGQRQLLTIARTMISNPPMLIL
                     DEATSSVDTLTEKKIQQAFLAMMEGKTSFVIAHRLSTIKNADQILVMDQGAIVEMGTH
                     DELLQKDGYYRRLYQAQFTAQ"
     gene            complement(29922..31637)
                     /locus_tag="IWO89_000866"
     CDS             complement(29922..31637)
                     /locus_tag="IWO89_000866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ0455639.1"
                     /translation="MIQLLRYAKDYRKQIILGPFFKFLEAVFELVLPLMMASLIDNGL
                     KMNDRGKIIEMGLWMVAMSVIGLICAIICQYYASVASQGFGTELRNQLIKKINTFSHN
                     ELNHFGTDTLITRMTNDINQLQLALAMVIRLLIRAPFLSIGSVVMAFVIDWEVGLFFL
                     ALLPIFSIILFFIIKKTVPLYQKVQEKLDQLNEHVSQNLSGVRVIRAFAQTKKETEKF
                     DKSTNDLANNYLRVSNISALLSPATTLIMNVGIICLLTVGGIKVNIGSLQQGQVLALI
                     NYMNQMLLALIVVSNLVVIFTRAEASGNRVKEVLDTENSILEAEVSTTPDFSSEAIIA
                     FDHVDFRYTPDSGLSLQNITFQLKRNTVLGIIGPTGSGKTTLTQLIPRFYDVSSGTVI
                     MDGMDVRSWQLDHLRQQIALVPQTSVLFTGTIRENLQWGKKDATEKECWEALKIAQAE
                     EFVRQLPNGLDTKVMENGKNFSGGQKQRLTIARALIAKPALLILDDSLSALDYQTDLD
                     LRKALQTYLGSTVIIISQRVRSIQEAHHILVMDQGKIAAQGTHEELLAQSAEYQEIVA
                     SQEEE"
     gene            complement(32416..32640)
                     /locus_tag="IWO89_000867"
     CDS             complement(32416..32640)
                     /locus_tag="IWO89_000867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002367150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0455640.1"
                     /translation="MKSDKYKNRKHTSWKTFREIIGSYLAVVVFLPLLYMAMTEQTRF
                     IGVDTQYIAETGFLFIFILIFAFYKSFHTK"
     gene            32840..35608
                     /locus_tag="IWO89_000868"
     CDS             32840..35608
                     /locus_tag="IWO89_000868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAQ0455641.1"
                     /translation="MLKKEILCYLENQTAFFDLEHMNEVFTAQRLADHFGVKRNTVSH
                     YLNQLNDEQLLVKINSRPVIYFHKEAFEKQFFKLRTNFYFSIDDLKEEQPFFAQPKDL
                     FSFMIGYDASLKESIEQIKTALYYPDGGLPLLITGESGTGKSYLVNLVYQYCLLHDLL
                     EDSAPFITFNCAQYADNPELLTSNLFGHVKGAYTGAEENKKGAFEAADGGILFLDEVH
                     RLTPEGQEKLFTYLDQGVIYRMGEPNLSRRIKTRLFFATTEEITSHFLTTFIRRIPIR
                     IELPALNQRSRNERLELIYSFFLEEQRKMGSPLSVSGPALSLLTSQKLSGNIGELKNI
                     VKVSAAKAYAEQREQPEIHVTIHHLQKELLAQPITKNTAQQGIYITQDQTLEQLMEKQ
                     QPEQQRIVKSFEQILIDFKKNNCLLYSCEGKLKQEVIQLFDFLLFETSRQEKHELLVY
                     WTQSIRETFRQMETAYQIKFDGNSVYALSYYLYQRSTIKWLPEEHDIIQLIKELEQQV
                     SQSYPSSYHYVQRILELSKIKLDIEDTSMDRILLTIYLKKAKWSKEKRLPKAMIAAHG
                     YATASSMANVVNRLLQEDLFESFDMPLDVTPQQIASEILDYCENSDVSNGLIILVDMG
                     SLKEIHQFFKKQLSVPLLILNNVTTPLAITVGECLQKNVSLEEIAEETVRQIQPEWRL
                     LYPEENKPKALITTCFTGIGTAVHLSELLEKSLPTACQLKIIPYEYQQLKDKKNSDPL
                     FSIYEVVGMIGTTDPDITNIDYLSLEDLISGEKMSVLAEWLDTTMNASEKEIFNQRII
                     RNFSLEKVLDSVTILDTEKVMGEIDHFMRELEVQLNRSISNPKKLALYVHVSCLIERL
                     IRQMPIETYQGLEKWKQCQKEGLSAIKSAFSVIEEHYSVMIPNSEIAYIYDILSEDTE
                     FLSIEEEF"
     gene            35689..36108
                     /locus_tag="IWO89_000869"
     CDS             35689..36108
                     /locus_tag="IWO89_000869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IIA"
                     /protein_id="HAQ0455642.1"
                     /translation="MDREIILASHGKFASGILDSLELIYGKNHSITVFDCYTDENFDL
                     AETVRQLFMRYKDKEIIVLTDLFGGSVNNEFLQYINRDHVYLVAGLNLPLLIELVSRL
                     DAEVETTVLIQQVLTDSKKMIQFCNERLNQKIEEDEF"
     gene            36120..36590
                     /locus_tag="IWO89_000870"
     CDS             36120..36590
                     /locus_tag="IWO89_000870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAQ0455643.1"
                     /translation="MIVLTRVDHRLLHGQVAFSWTQTIGADCILIANDDVPTNEIRKT
                     TIKLAKPQGVKLVIKSIDDSIAALKSGVTDKYKLFIVVESIEDAYKLAEAYPEIKTIN
                     LGGIKAKPGTRSIGKAVNILPEEEERLKRLIDKQIEVEIRQVPADKKVDVREVL"
     gene            36607..37386
                     /locus_tag="IWO89_000871"
     CDS             36607..37386
                     /locus_tag="IWO89_000871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAQ0455644.1"
                     /translation="MIVQAVLLGIVAFVAQSEYALGTSLLSRPIVTGLFTGIVLGDVK
                     TGIIMGATLELAFIGSFSVGASIPPDVVTGGILGTAFAITAGAGTETALLLGLPIATL
                     TLILKNIYLGLLIPIMNHKADNYAEEGNYKGVEHMHLLAGIGLSLMLGVVVMVSYMVG
                     SNTIGNLLNMIPDFVQKGLSVATGLIPALGFAMLARLLINKQVAPYFFLGFAIASYLE
                     IPVTGVAIFGAILAVVVVNIMNVRQLPLQTTAGEVEEDEEF"
     gene            37373..38194
                     /locus_tag="IWO89_000872"
     CDS             37373..38194
                     /locus_tag="IWO89_000872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HAQ0455645.1"
                     /translation="MKNSEIKNSKITKKELNHVFWRSFQMEFSWNYERQMNMAYVYAM
                     IPILKKLYQSKEEMAAALKRHLEFFNTTPHIVTLILGITAAMEEENKDDPEFDVTAID
                     SIKTSLMGPLAGLGDSFFWGTLRLIATGVGTSLAMQGNILGPILFILIFNIPHILFRY
                     LATGWGYRLGTGFLKKIQANGMMESLTLGASIIGLMVVGAMTATMITINIPLKIGSGE
                     NAVTVQSIFDDIVPNILCLGAFGIVFYLLKKEVKPLTILIGLAFFGIFGSWIGIF"
     gene            38211..39260
                     /locus_tag="IWO89_000873"
     CDS             38211..39260
                     /locus_tag="IWO89_000873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HAQ0455646.1"
                     /translation="MFTMQDYIYEEKEVLSTILKKNDFSTREHMKKTVNLLILATGSS
                     YNACLAAKPALESYGDLTVDIQEPFYFFNYGKLSPSIDTVIAVSQSGKSASTVEAVKM
                     IQKQGLPVVAITNDVQSPLALEAAQIIDLGAGVESVGFVTKGYSATVLQLLLLGIGIG
                     ISKNKISKKIEQDYMQQLRKIINHLPAIIQKTEEFFDGYQSLFRLAQRFVTIGYGPNW
                     GTAKEAETKLTETIRVPSQGFELEAYMHGPYLEADASHLLFFIEGESVNKERSQKLQR
                     YMSRYVGETLTITTKKARNEKTLGLAIECDEYLSVLALVVPFQLFAYKTAVAKGIDLN
                     KKIFEDFDTVLKSKL"
     gene            39273..40277
                     /locus_tag="IWO89_000874"
     CDS             39273..40277
                     /locus_tag="IWO89_000874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010760608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HAQ0455647.1"
                     /translation="MLKFNEEEQIKAIKGALALRPQVEEIIDKLYAEKFDAVYYLGIG
                     GTYASSMQAVTYMNGKSNLPVFVQHAAEYYTTGNKRLTKDSIVILSSVTGTTQEVVKA
                     VEQIKKVGATLIGFIDKANSKLSQLCDFVVTYPAPGTEQIKFFMAADRLMYLNGEFEA
                     YSEYYEQLERYLPTGLVEAEKKADAFGLSFAEKHRRDSMHYFIGAGNQWGAVYSYAMC
                     YWEEQSWLPSKSIHAAEFLHGTLEIVEETTPVTLFLGEDEQRVLSERVAKLLPKICSN
                     YTLIDTKDYPVEGISEKYRGRVLSFLLMHVVTQRIDAHVEQLNCHPLEIRRYYRQFDY
                     "
     gene            40910..41929
                     /locus_tag="IWO89_000875"
     CDS             40910..41929
                     /locus_tag="IWO89_000875"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HAQ0455648.1"
                     /translation="MKQKIAVLGPGSWGTALAQTLAENGHDVRIWGNVPEQIDEINKY
                     HTNQHFLPDLTIPESIIGYKELAEAVDNADAILFVVPTKAIRSVAQELIGKMNTKPII
                     IHASKGLEQDTHKRISEVLAEEIPEEKRQAIVVLSGPSHAEEVAVHDITTITAASIDQ
                     KAAAYVQKLFMNDYFRIYTNNDVIGVETGAALKNIIAIGAGAIHGLGFGDDAKAAIMT
                     RGLAEISRLGVAMGANPLTFIGLSGVGDLIVTCTSVHSRNWRAGNLLGQGHKLEEVLE
                     NMGMVVEGVATTKAAVELAQQLGVEMPITQTIYNVLYNGEDIKKAAKEIMLRDGKMEN
                     EFSLR"
BASE COUNT        13778 a         7381 c         8684 g        12164 t
ORIGIN      
        1 actactcgaa actaagcgac tttttcacaa cctcctttac ataattttat tttgttgtgg
       61 aaggggtttc gtgttattct aaaagaggaa cgattgaagg ttataggaaa ggaagagatg
      121 aatggaacac aaagaactta cctcatttcc aaaagatttt ctgtggggct ctgcttctgc
      181 agcgtatcaa gtagaaggag cttggcaaga agatgggaaa ggagaatcag tctgggatcg
      241 atttgtacgg atacctggaa agacatttaa aggaacgaac ggtgatttgg cagtagacca
      301 ctatcatcgt tataaagaag atattgcttt aatgaaagaa caaggtctga aagcttaccg
      361 cttctctgta gcttggacac ggatttttcc taatggacga ggagaagtca atcaagctgg
      421 gctcgctttt tatgagagat tgatcgacga attgattgaa aacgatattg aacctatgtt
      481 gactttatat cattgggatc ttccacaagc attgcaagat gaatataatg gttgggaatc
      541 acgtcagatc attgaagatt tcactcatta tgcagaaaca ttatttgagg cattccgcgg
      601 gaaagtccat tactggatta gtttgaatga acaaaatatc ttcacttcat taggttattt
      661 gttagcagca catccgccag gtgtgacaga cccgaaacga atgtatgaag ttaaccatat
      721 tgctaattta gcaaatgcat ctgtcatcaa taaatttcac gagttagaaa tgcctggaaa
      781 aatcggacca agctttgcgt atacgccaaa ttatccaatt gacagtaatc ctgaaaatgt
      841 tctggctgct gaaaatgcag aagacctgat ggccaattat tggatggatg tctatatgtg
      901 gggaaaatat ccgattgctg ccatgaggtt cttagaagaa aaaggatggg caccaacgat
      961 tgaagctggc gatgcagaat tgctagaatc tgctaaacca gattttcttg ggatcaatta
     1021 ctatcaaacg gcgacgaatg cattcaatcc attaaacgga gttggtgctg gaaaaatgaa
     1081 tacgacagga aaaaaaggtt catcagaaga aactggagtt cctggaatgt ataaaaaagt
     1141 tgagaatcca tttgtcgaac gaacaaattg ggactgggag attgacccag aaggattaag
     1201 gatcggactg cgtcgtatca caagccgtta ccgcatgccg gtactgatta cggaaaatgg
     1261 cttaggagag tatgacaagt tgacagaaga taaacaaatc catgacgact atcgaatcaa
     1321 ttacttacaa agccacgtca aagccatcaa agaagcaatt tcggatggag cagaagtcct
     1381 aggctattgc acctggtctt atacagattt gcttagctgg ctgaacggtt atcaaaagcg
     1441 atatggattt gtatatgttg atcaagacga aacacaagaa ggatctttgg aacgatataa
     1501 gaaagacagt tattactggt accaaaaact gattgaagaa aatggagaga acctttagtt
     1561 cacatactta aaacaagaag gtacggctgg atatggccgt gccttttctt gataaaatca
     1621 tttagaaatt aagaaaaact gtaaaataag ctgaaaaatg aaagaaagcg tcagttcttt
     1681 caaacaaaac tttagttccc ttactttttt ttgtataatg aaacttgatg aaatgaggga
     1741 taataaatgg cacaaaagaa acggcctact aaaaaaagga aaaagacgaa aaaaacgaaa
     1801 aagcaaaaac agcagcagga acaaatgctg acgattggtg ttgggtttgc cttgatttta
     1861 tttgctgtct tcggcttttt aaaattaggt tttttaggaa tattgattgc aaacggcttt
     1921 cggatcattg caggaaatac ttaccaaatc ctctgtcttt tattagccgt attaggattt
     1981 tggattgtca ttaagaatac agagttttca attggtaaaa accgacgctg gtttggcggc
     2041 atactctttt atttcggtat cttattattg ttacatgccc atttatttgg caaactccat
     2101 actggggaac caaatatcat ggggacgaca tgggatttgt tagccagtga tataaaacaa
     2161 agtcaagtag acaataacgt tggtggtggg atgattggag caatccttta tcattttact
     2221 tacttcttga ttgcacagcc tggaagttat ttagcagcag tgctattact ggcaggagga
     2281 gcattcctgt tcagtaatct ggaaggctat cagctgttaa atggtatcca atcaattgga
     2341 gaacgagtac aggaattatt ggaaggtgat cctgccaagc aagctcgaaa gcaagcggca
     2401 aaggaagaga gaatgaaaca gcgcgcagaa gcgaaagaag caaggcgctt agctgcaaaa
     2461 gaagctgcag aaaaagaagc tgcagaatat gagaagaaaa aagcggtgag ccagcaaagg
     2521 gaaaatactg ctgatgaatg gcaggaacca gaaaatacag agccggagca gctaagtttt
     2581 gttccaatcg acagttttca agaaaatatc catcctgcga atctagagaa accagtacca
     2641 gatacaccaa aacagaccaa tacagcagaa ggtttttcag atgagttgcc agaggatgac
     2701 ggtacctcat tagaatttga gattgaagca gaacaggaaa atcaagacta tgaattaccg
     2761 acagttgatt tgcttgattc tataccaact gtggatcaaa gtgatgagta taaaaaaatt
     2821 gaaaaaaata tcggcgtatt agaacaaact ttccaaagtt tcggtgtcga tgccaaggtc
     2881 gtcaaagcca gcttaggacc atccgtcaca aaattcgaag tccagccggc tgtaggggta
     2941 aaagtaagta aaattgtgaa cttaacagat gatatcgcgc ttgctttagc ggcgaaagat
     3001 gtccggatgg aagcaccgat tccaggtaaa tcactgattg gtattgaagt accaaatgga
     3061 aagatcagta tggtttcctt tagagaaatc atcgaagcac aaccaaacca cccagataaa
     3121 ttactagaag tacctttagg acgtgatgtt tctggacggg tacaaacggc tgatctttcg
     3181 aagatgcctc acttactcgt ggctggttcg actggtagtg ggaagtcagt agcaattaat
     3241 gggattatca caagtatatt aatgcgtgct aagccccatg aagtaaaact gatgatgatc
     3301 gatccgaaaa tggttgaatt gaatatgtac aacggcatac ctcatctgtt gacgccggtc
     3361 gtgacgaacc ctagaaaagc agcgcaagcc ttacaaaaag tagtacaaga aatggaagaa
     3421 cgatatgaaa aattcgcggc aaccggagta agaaatattt ctggttacaa tgaatttgtt
     3481 caacagaaaa acttggaaaa cggaacgaaa catccaacct taccattcat cgtagtgatc
     3541 gtagatgaat tagctgattt gatgatggta gccagcaatg aagttgaaga tgcgattatt
     3601 cgtttagcgc aaatggctcg ggcagcgggg attcatatga tcctagctac tcaacgtcca
     3661 agtgtggatg ttatcacagg aatcatcaaa gcaaatgtac cttccagaat ggcatttgcg
     3721 gtatcaagcg gtacagattc tcggacgatc attgattcta atggggcaga aaagctctta
     3781 gggcgaggag atatgctatt cttgccaatg ggtgagaata aaccgatccg tgtgcaagga
     3841 gcattcattt ctgaccacga agtagaacgg gtcgttcaat ttgttacaga tcagcaagaa
     3901 gctcattatg aagagaaaat gatgccgaca gatgaagttg aaacgacagg tgctccagaa
     3961 cagccacaag atgagctgtt tgaagaagca aaagcacttg tcgttgaaat gcaaacggca
     4021 agtatttccc ttctccaaag aagatttcgg atcggttaca accgagcagc acgtttagta
     4081 gatgaactgg aagctcatgg agttgttggc ccgtcagaag gaagcaagcc tcggaaagta
     4141 ttcattgagc aggaagaaac atcagaaacg ttgccggacg ataatgacat gtaaaacttg
     4201 tgtgatgaag ggttgaaaaa cgaataacca ataaaaagac aaggcatgat gataccaaaa
     4261 gaaaaaatgg tttttttcca tcttttctaa tacggtatga tgtcttgtct ttttagtttt
     4321 tgtgagatta aagttctatt cgatttattt gtgaagtatt gcattaagga aatgaatcat
     4381 gtatagtatg tagcgaaact ttgagaaaaa gagggaaaat actttggaaa aacagtccat
     4441 ttatggcttg acgaatgaag aattaatcaa ttggtttata gaaaacggcg agaaaaaatt
     4501 ccgagctgcc caagtttggg aatggttgta tcaaaaacgt gtaagtaatt tcacagaaat
     4561 gacgaatcta tcgaaacaat tgatcgaaaa attatcggag cattttatca tcaatcctct
     4621 aaaacagatg gtcgtccaag aagcttcgga tggtacagta aaatatttgt ttgaactccc
     4681 tgataaaaac atgattgaga ctgtactgat gagacaagaa tatggcttgt cagtttgtgt
     4741 gacgacacaa gtaggatgta atatcggctg tacgttttgt gcaagcgggc tgctcaaaaa
     4801 gaaccgtgat ctgactgctg gagaaattgt tgcacagatc atgatggtcc aacattattt
     4861 tgatgagcgc aatcttggcg aacgcgtttc acatgtcgtt gtaatgggga tcggtgagcc
     4921 atttgataat tacgacaatg taatggactt tttgcatatc atcaatgatg ccaaaggatt
     4981 ggcaattggt gctcggcaca ttacagtttc cactagtgga ttagcacaca aaatcaagga
     5041 attcgctaat aacggcttgc aagtcaatct ggctatttcc ttgcacgcgc cgaataatga
     5101 ggtgcgaaca tcgatcatgc ggatcaaccg aagtttccca attgagaaat tgatggaagc
     5161 tgtggatgag tatttagaga aaacaaaccg tcgaatcacg tttgaatata tcatgttgaa
     5221 tcaagtaaat gatagaccag agcatgcgca acaattagca gatctattaa aagataagaa
     5281 aaaattagca tatgtcaact tgattccata taatccagtc agtgaacatg atcaatattc
     5341 tcgcagtcca aaagctgatg tattgagatt ttatgatgta ttgaagaaaa acggcgtaaa
     5401 ctgtgtgata cgtaaagaac atggtacaga tattgatgca gcttgcggac aattgcgaag
     5461 caagcaaatg aaaaaaacag ttgccaaata atcgtatcct actcgttttt tcaattacca
     5521 taaaaggagc agttttctta attaaggaac tgttcaactt aaataagaga aaagattcta
     5581 tagcgaaagc tataaagctt tttttgacaa gaaaaaccct catcaagcaa agaaatgtcc
     5641 tcaatttgat gacagcctgc ttgatggggg ttttagcaat tatttaactg attttcttat
     5701 atccttgctg gctttcttgt gtagcatgat aagtttctac gtcaagaatg gcctgtttaa
     5761 atttttctcg ggtgatttca taaggcacat gtttaatatc atttgttgcc atggctttat
     5821 ctaagattgc ttctaaatcg gtcgtgacat tgagattcat gtcggttaac gatactggaa
     5881 gttggttctt tttcataaac acatagattt tatcaaacaa atcgaactgc tgatccatca
     5941 tcaataaggt cagtacacca taactgacag atgctccgtg aaggtatccc tccatttttt
     6001 ctagaacagt cgtaccgtaa tagaatgagt gtgccatagc agaattatag ttattgttaa
     6061 ctaaaacaga aaccaatccg gtagtaacaa tgatatccga aacgacttgt tcaattgcat
     6121 atgagggacg atcgtcggca ctatctttca atgcttgcgt accataagtc aagagacgat
     6181 caatgcacat ttttccgatt tggattccta atctatccgt gtagcctaga tcatcgccac
     6241 gagcagaaaa agtagattcg acttctttgg ataaagcatc gccaatacct gcccataagt
     6301 atttatcagg tgcttcggca ataattgctg tatcaatgaa ggtgtgtaca ggtggttttg
     6361 aaggaaagaa gagtccgtcc attacgcctt cttcgttata gatgacacat accgcagtga
     6421 cgggcgaaca gttcgaacca atagtgggaa aggtgaagac tggtttagct agtttttcac
     6481 caatgacttt tacagtatcg caagcacggc caccaccgac tgcaaacagg acgtctgctt
     6541 tttggacact ttcatgctgg atcaactgtt caatatttcc ataagttgaa ttacccccgt
     6601 accagagact ggcgacaacc tctatttcct gtgtcaaagc tacttttatt ttgttttctg
     6661 cctttgaaag tgcagtcttc cctccaatca gcactacttt tgtcccatat tctttgacga
     6721 ttccatttgc tttttcatac gcatctgttc caatggtata atttggtaag aaaacacttt
     6781 gactcatatt catttccttc tttcttcgtt gaaacgggtt aaaattttaa agagtgatcg
     6841 atctgactgc ggctgtagat ggagtagagg tgaacctaag acaggataag tgatgaataa
     6901 ggtggcatga acaccttgaa atcatcaata agtatcctaa aaacgaacaa gtacaaaaac
     6961 attgaaaacg acaataatga atttttgttc ctcttagtat ttctaccgat tccctttgct
     7021 tttttgtaat tatctaaaaa ttctaaaaat taagattatt atatgaaaac aggttttatt
     7081 tgtcaattca taattggttt tttttaaagc aaaagataat ctatatcatg atgaacaaac
     7141 agagtttttt cctccaattg atggctttcc tgatctttcc tattttgaat aaagtgtttc
     7201 tgtataattt ttttatttta taagtaagat aaataatgaa agaatagaga tactgattgg
     7261 tagctaaaga tttttgagcg agaacatttc gttttttcaa aaggactgtt agaataagca
     7321 agaacaaggg ggaagacgta tgataatcag tgaatttgac cgtaataatc cagtattgaa
     7381 agatcagctt tctgatttac tgagactgac ttggccggaa gaatatggag acagctcggc
     7441 agaagaagta gaagaaatga tgaatccaga acgaatcgcg gtagcagcgg tagaccaaga
     7501 tgagttagta ggatttattg gtgcaatccc tcaatacggt atcacaggtt gggaattgca
     7561 tccattagtt gtagaaagct cccgacgaaa gaaccaaata ggtactcgat tagtcaatta
     7621 cttagaaaaa gaagtagctt ccagaggagg aatcacgatt tatttaggta cggatgattt
     7681 agaccatgga acaacgttaa gtcaaacgga cctgtatgag catacatttg ataaagtggc
     7741 ttccatccag aaccttcgtg aacatccgta tgaattctat gaaaaattag gttataaaat
     7801 cgtaggtgtc ttaccaaatg caaatggctg ggacaaaccg gatatttgga tggcaaaaac
     7861 gattattcct cgaccaaatt ctcaataata aaaagaggtt gtgacagaag taatcagctt
     7921 caagaaataa gaaggaattt acgaaaatta cttcttgaat ttttgtgaaa ttccaactta
     7981 tttccgaaga agctactttt ggaacaccgt gtaccagagg ttgtgaaaaa gtcgcttagt
     8041 ttcgagtagt aagaaaaatt ttgtgaaaat ggctttctac atttttgcat aaatttggct
     8101 taataccgag aaactggctt ttgaacaccg tttattcaga taagggactg tgacaagact
     8161 tttgtcacag tttctttttg tttattcttt tttatctttg tatggcaata atagacaata
     8221 taacgaaaat tatttaatgg cttttggaag accatggaaa accttgtaca aagctatttt
     8281 ttctgaaaaa ttccttgaaa cctcacgaaa aaatcattat tttatgaaaa agacaaaaaa
     8341 aagtgttgat tttttaataa ttattcatta aactcttaaa caacttcaga aaatattaat
     8401 tattctgaaa attccaataa aggggtacgt aaaatgaaaa agaaatttac atttggtttg
     8461 gttgcggtat gcggccttgt acttgctgga tgttatggcg gcagttcagc cacaagcagt
     8521 agttcagcaa aaagcggttc agcagatgga ggaggcgtat ttaacttagt cgtgcctcaa
     8581 gaaatgccta cagctgactt atcagttgca acagatacaa tcagttttac agcattgaat
     8641 aacgtttatg aaggaattta ccgtcttgat gaagacagca aaccacagcc ggcaggagct
     8701 agcgagttgg cagaagtcag tgaagacgga ttaacttaca aaatcaaatt acgagaagac
     8761 gcaaaatggt caaatggtga tcctgttaca gcagcggatt acgtttatgg ctggcaacga
     8821 acagtagacc cagctacagc ttctgaatat gcttacctgt atgcgccagt tgaaaatgca
     8881 gaagacatta cagctggaaa gaaagataaa tcagaactag ggatcaaagc agttggtgac
     8941 tatgaattag aaatcaaatt gacgaaacaa acaccatatt tccaatatct gcttgctttc
     9001 ccatcatttt tcccacaaaa acaatccgta gtggaagaaa atggggatgc ctatgcatct
     9061 gcaagtgata aagctgttta taatggacca ttcacattag aagattttga tggaccagga
     9121 acagatacag aatggactta taagaaaaat gatcattact gggacaaaga tacagtaaaa
     9181 ctttctgaaa tcaaagtcag tgtagtaaaa gaatcttcta ctgcattgaa cttattcaaa
     9241 gatggccaag cagatgacgt gatcttgtct ggtgagttag cacagcaaaa cgcaaacgat
     9301 ccagcttata cttctgttaa agaagcgcgg acaagctata tcgaattcaa tcaacgtgaa
     9361 aagaattcac cattcaacaa tgtgaaccta agaaaagcta tttcttattc tatcgatcgt
     9421 gaagctttag taaaacaagt actaggtgac ggatcagttg tctctacagg attgatccca
     9481 tcagatatgg taaaaagtcc aacaacgaac gaagactttg caaaagaagc tggaaaatta
     9541 gtttcttatg acaaagataa agcaaaagaa tattgggaaa aagcgaaaaa agaattgggc
     9601 gttgattctt tagaatttga tttgatggct tctgatgatg attcatcgaa aaaagtaatc
     9661 gaatatgttc aaaactcaat ccaagaaaac ttggatggcg taacagtcaa accaacacct
     9721 gttccattct ctgtacgtct tgaccgctca acttcgggtg attttgacgc agtactaggc
     9781 ggatgggctg ctgactatgc agacccaagc agctttactg atttgtttgt cacaggtaat
     9841 tcatacaacc gtggtcgctg gagcaatgca gaatatgacg aagcagttaa agcagcttca
     9901 tcaacagacg ctggcgatga acaagcccgt tgggatgatt tccaaaaagc aatcaagatc
     9961 atctctgatg atatgggtgt cgttccggtg taccaaaaag cagaaggtca cctagtgaat
    10021 gaaaaaatca aaggtatcgt tcaccatgcg gcaggcgctt catgggatta caaatggaca
    10081 tacgttgaag aataatcgtt aataccgtga gcatgagaaa aaacgaggct gcggcaaatg
    10141 tcgcaacctc gttctcatat tcacttatcc taacaaagaa aaaagcatga aaatagcaga
    10201 aagagcaatt agtattccgg caatgatcag ccaaaagctg tacataccgt agctggcaga
    10261 cgagagcgag gaaaattctg gttttttctt tcggttcctt gttcgtgcaa gatggaccga
    10321 tcgctgaaaa tgatcgaaga aacccgaaga aaagacccat aaaaaaccgc cgataatcag
    10381 aaacggcaat gaccataaga atagatcatt agataattga atgagtgtaa aggaatggtt
    10441 gatgagattt tttattaaaa taaccagaat cagtagaaaa gctactgcgt aggggataga
    10501 tttttttttc atttgattgc tcctttattt ttggttcatt atctaataat gtagcgaaac
    10561 atctgtcaga agtcaaaagc tagggggaaa cattagtgaa tagttatatc aaatatgtat
    10621 taaaacgtgt ctttttcatg atcatcacgc tttggttaat tgcaacaatc acctttttct
    10681 tgatgcaatt gctgccgggc acgccatata cgaatcagga acgtttgagt cctgaaacaa
    10741 tcgagatgct aaataaacaa gtcggtttgg ataaaccagt tatcgtacaa tatgggattt
    10801 atctttctaa tttgcttcaa ggagattttg gaatttcctt ccaatttaaa aaccagccag
    10861 tcgcacattt gttagctgga agaatcggac catccttgca gttagggtta caggcaatta
    10921 tctttggaac agtcctaggg acgattttag gaacgatctc tgccatgaag caaaatacat
    10981 gggcagatac atcttctaca ctcgtagcga tattaggtcg ttccattcca aactttgtat
    11041 ttgctgtttt attacaatat atctttgcaa tcaaattaca agttttgcca atcgcaaaat
    11101 gggatggctt tgtgtacaca atcttaccaa caattgcgtt ggccatgtcg ccgcttgctg
    11161 actctgctcg gtttatccgt acggaaatgg tggaagtact gcatagtgat tatgtggaat
    11221 tagcaaaagc aaaaggattg agccgttggg aaatcgcctt taaacatggt cttcgaaata
    11281 gtttgattcc attgatgact ttattaggac cgttagctgt agcattgatg accggatcac
    11341 tggtggtaga aaatatcttt gctattcctg gtatcgggga acaattcgtt aagtcaatca
    11401 caacaaatga ttatcctacg atcatggctg taacgatcct ttactcattt atgctgattt
    11461 ttgttatttt gatcgtggat ctattgtacg gtctagttga tccaagaatt cgtgtttcag
    11521 aagggagccg aggataaatg gaaatgatac caaagcgtta tgaaacagtt gcagatatcc
    11581 ctgcggatga atttcttcca ttacaaaaaa ataccgaaga agaacgtgaa cagattactg
    11641 caccttcttt gaattttatc caagattcat ggcgccgttt aaagaaaaac aaagctgccg
    11701 ttatttcaat ggttttacta ttggtcatca ttttgatctc tgtgatcaca atctttgttt
    11761 ctcctcatga tccaactgcc caaaacgtgg cttatatcaa tttgcctcct cgtattccgg
    11821 gaatcaatat cgatggattg aatggaaaag caatggttgg tggtgaactt gttgataaat
    11881 atgctcaagc aaacgtccct tcaaacgtaa atttcttttt aggaacagac ggtttgggac
    11941 gtgatgtatt aagccgctta tttatgggaa tgagaatctc gttgctgatc gcatttattg
    12001 ctgcattact agatgtaacg attggtgtag cttacgggtt ggtttctggt ttgttaggcg
    12061 gacgtgtcga taacgtgatg caacgttttt tggaaatctt atcaggaatt ccaaacctcg
    12121 ttgtcatgat tttgatgttg gtcgtttttg aaccaggaat tttttcaatc gtggctgcta
    12181 tggccatcac aaactggata ccaatggcac gtatcgttcg tgcacaaaca atgaaattaa
    12241 aagatcaaga atatgtttta gctggattga cattaggtga atcaagaatg aaaattgcat
    12301 tcaaacatat tttgccgaat atctctagtg tcattattat tcaaatgatg tttagtattc
    12361 catctgctat tttcttcgaa gcctttttaa gctttatcgg tttaggtctt acgcctccgt
    12421 cagcttcatt aggaacgatg ttgagcgaag ggtacaagac attcctgtat ctgccttatc
    12481 tattatggat tccggcagta actttatcgg ttatcatgat tggctttaac ttacttgctg
    12541 atggattgcg tgatgccttt gatcctaaaa tgaaagagtg aagacgatga cgaaaatttt
    12601 agaagtgaaa gacttagaaa tctcttttga tacgtatgct ggaaaggtac gagcgattcg
    12661 cggtgtcaat tttcatttga ataaaggaga aacattggcg atcgttgggg aatctggaag
    12721 tggaaaatca gtgacgacca gaagcatcat gcgcttgctg tcaagcaacg ccaatataga
    12781 tgctggtcaa atcctcttta aaggtaacga tatcgttgga aaatcagaaa gagagatgca
    12841 aaaaatccgt ggtaaagaaa tagcgatgat tttccaagat ccaatgacct ctttagatcc
    12901 gacgatgccc attgggaagc aagtcgctga gtcccttaga aagcataata aagtttcaaa
    12961 aaaagaggga caaaaagctg cattagaatt gcttaaatta gttggaatac ccgaagctga
    13021 gaaacgtatc aacagttatc cgcatcagtt ctcaggaggt cagcgtcaac ggatcgtgat
    13081 cgcgattgca ctggtatgtt atccggaaat cctgattgct gatgaaccta caacagcgct
    13141 tgatgtgact attcaagcac agattttgga gctgctgaaa gaaattcaga caaaaatcga
    13201 tacatcaatt atctttatca ctcatgacct tggtgtagta gcgaatgtcg ctgatcgtgt
    13261 cgctgtcatg tatggaggaa gaatcgttga agttggaaca tcggaagaaa ttttctacaa
    13321 cccgcaacat ccctatactt ggggactgct tggttcaatg ccgacattag atagtgcgaa
    13381 cgatcgattg tatgctattc ctggttctcc accggattta ttgaacccgc caaaaggaga
    13441 cgcattctat ccaagaaatg aattcgcgat gaaaatcgat gcagaacaag aaccaccatt
    13501 ttttgaacta tcaaaaacac acaaggcagc aacttggcta cttgctccgc aggcacccaa
    13561 agtgacaccg ccagaagaaa tccaacgtcg ctgggctatc tttaaagaga aacaaaaaca
    13621 tacttacggt caaggaggaa ttgctgatgc caaataaact acttgaagtt aaaggattga
    13681 aacagtattt caatgtagga aaaaagaatg aagtacatgc tgtaaatgac atcagctttc
    13741 acatttatga aggagaaaca ttcggtttag ttggagaatc tggaagcgga aaatccacta
    13801 ctggccgtac gatcattcgc ttgaatgaac ctactgatgg tgagatatta ttcgacggtc
    13861 aagatataac gaagatcaaa gataaaaaag gtttgacgaa attccggcat gatgttcaga
    13921 tgatctttca agatccatac gcttcgttga atcctcgtat gaaagtcaga gatatcattg
    13981 ccgaaggaat tgatgttaat ggactagcta aatcatctga agagcgtgca aaaaaagtcg
    14041 atgatctatt gagaactgtt ggattgaatc caagccacgg tactcgttat cctcatgaat
    14101 tttcaggtgg gcaacgccag cgtattggta ttgcacgagc attggcagta aaaccgcgtt
    14161 ttatcatttg tgatgaacca atttctgcac tagatgtgtc gatccaagcc caagtggtca
    14221 atttgttgca agatctccaa aaagaacatc aattgaccta tctatttatt gcacatgatt
    14281 tgtcgatggt taaacatatt agtgaccgaa tcggtgtaat gcataacggc ttgttattag
    14341 agatgggaac aagtgatgaa atctataatc acggtgttca tccttataca gaaagtttgt
    14401 tatctgctat tcctctacca gatccagacc atgagagaaa acgcagaaga atcaaatatc
    14461 aacctgaacc agatgacgga caagtacgtc aacttcgtga aatcgcacca gaacattttg
    14521 tctatgcaac tgagcaggaa gtcccatatt acgcaaaaaa actaaaacga caaaaggaat
    14581 ctcttctggt tgctaactag gagggacata gatgctaaaa gcttttaaat tccgtatgta
    14641 tccgacagaa gaacaaaaac agcaattgat tcgtactttt ggatgtgctc gatttaccta
    14701 taaccatttg ctgaaaaagc ggcagaaaag ttggcaacaa acaggagtag ccaatttttc
    14761 gctcacaccc gctacattaa aaaaagaata cccattctta aaagaagtag acagtcttgc
    14821 tttagcaaat gcacagctta atttggatcg ggcttttcgc aattatttca agggacgtgc
    14881 cagcttccca aagcttaaaa cgaaaaaaag tatgtggcaa tcttatacga ccaacaacca
    14941 aacccgtacg atctatctgg aaaatggcta cttaaagtta ccaaaacaaa aagaactaat
    15001 caagataaat cagcatcggc cagttgaagg atcgattcgc tccgctacga tttctgctcg
    15061 atataatgaa gaattttatg tcgcactact ttgtgatgtt tcgccagtaa aaaaagaatc
    15121 tttagctaaa tggatcggga tcgcctatca tccgaagaca ttgatccaaa catctcgacc
    15181 tctagaagta actttaccaa aatttcacca gacagaagaa aagctgcaac atgcacagcg
    15241 gaaattgaat gtgaaagtcc gctccgctca ccatagaaag atcaggttag atcaggcaag
    15301 caattatcag aaacaaaaga gaaaagtaat ggatctttat ctgaaacaaa agaaccagcg
    15361 agaagattat cttgaacaat tatctgggaa attggtcaaa caatatgact acttatttgt
    15421 agaatctttt ccaaaagaag aggcacatgc tgatttttct attcacgact ggcataaatt
    15481 aataacaaaa ttgcgttata agtcccaatg gtataataaa aaatttcttt tgatcaatac
    15541 cgacggagca gaagaaagca attccgtaag aaagagccaa gtagtagaaa aaatgggcag
    15601 acattcggtt atcaagggat aaaaaatgaa tgaaactgat aggagcgtta gggttagcct
    15661 tgttaataaa aagtcttaat gacttttgtt tctaggaatt tttcttttga agaaaagctc
    15721 actaattatg acaaaccaat aaaacaatag cagaaaaagt tcgtacgagc tttttctgct
    15781 attgttaatt ttttattcag aaagtttacg aatacatcaa aattctgaaa caaaaaacgt
    15841 aactcacaaa gagttgtttt tgcgtttttt cttgaatgat taggtctgaa tcttttgctc
    15901 acaaccttat tcatggtgcc ccggcttgag ctgatagatt tttgaaggac ggccgattcc
    15961 tacaggacgt tcaccgatct catcgaaata ttggagcata gctttcttaa aattggaatg
    16021 atcgatcgtt ttataatcga tccctaagaa tttagcaaat acttttcgag cttccgtaat
    16081 cgtgaaatct ttacctaaaa cttgtagaac ttgcggctcg tgttccattt tattgactac
    16141 acggttgaat gctttcaaga tgatttgact atggtcaaaa gccaaagtat ctttgccaga
    16201 agactctcca gtgactaaat caagcgagat agccacttct ccgctagata aatggatctt
    16261 gttactatga cgttctagtg tgaaccagcg tacttccttg gcatcatctc cggcgattag
    16321 cggttcttct ccaataaagg ctaagtagct gactgtgacg acccagcctc gcggatctcg
    16381 attaggcgta ctgaatgtat gcagttgctc aatattttct tttgaaataa ccacaccagt
    16441 ttcttccttt gtttctctta agacgctttc tccagttgac tcatcctttt ggacaaagcc
    16501 accaggcaat gcccatgaat ttcgataagg atgccctttt ctttggatca acaaaacttt
    16561 cagctgatct tcttctttgt tatagcacat cagtacgata tcgacagtca aagagggctt
    16621 gtcatattct ggacgttcct gtttttgata ccagttaaga aattcttctt cacttgcttt
    16681 atgttcataa tataattttt cttcttcttt agaagcaaaa cggttcatct gaccacctcc
    16741 attgacagta aaagtacttt ttaccttatt gtagcagact ctgaaaaaaa accaaggatt
    16801 tacttgaaaa atctaggtta tcatttaaat atgaaaaata aatggaagta tgagattgaa
    16861 taaaaagaaa agcagataaa acggcaatac tttataccgc tcttcacgaa tggcaaagaa
    16921 gccacaaata acgatatata cgtcaatgat aaaacgcaaa atccaagctg ctgtcataaa
    16981 actgttcaaa tgcccatgga ggttaaaata accatacata cgaatataaa aatagctgaa
    17041 tattaaatgt gctaatgaaa gaaaagcaat actaagtgta aaaaaaagat gtttcttctg
    17101 catgattgtt ccagtccctt actaagatag atagtcttta ttataccacg tgtgagggga
    17161 gaaataaaaa gccgtttttt agtagatatg aaacaaaaca aaaacccgca tttattcaac
    17221 ctattcatcc ttttaaagga tagattgaac gtagatagaa gtcgtttatc cagtcttttt
    17281 cattttgaaa aaaagctaac agcatgcttt ttttatggta tactagcaag gaaacgaatt
    17341 cgcatcatgc gataaaggag gaagagcccc gtggcagaaa aggaaacttt ttatatcact
    17401 acgccaatct attatccaag cggcaaactt catatcggca actcgtatac tacgattgca
    17461 tgtgacgcaa ttgctcgtta taaacggttg atgggctttg atgtgtttta tctcactgga
    17521 gtggatgaac atggtcaaaa aatcgaaaca aaagcagaag aactaggtgt aaaaccgcaa
    17581 gaatatgtag ataaaatggc ggctgatgtc aagaaattat ggaaaacgct tgatatcagt
    17641 tatgataaat tcattcgcac gacagatgat taccataaag cagctgttca aaaaatattt
    17701 gatcgtttat tagaacaggg agatatttac ttaggtgaat atgaaggctg gtactctgtt
    17761 tctgacgaag aattctttac agaaacacaa cttgcagaag tgtacaaaga tgacgaaggt
    17821 aacgtgatcg gtggtaaagc gccaagtgga cacgaagtag aattggtaaa agaagaatca
    17881 tacttcttcc gcatgagcaa atacgcagac cgtctattgg aatactatga agaacatccc
    17941 gaattcatcc agccagaatc tcgtaaaaac gaaatgatca acaactttat caaaccagga
    18001 ttagaggatc ttgcagtttc acgaacaact tttacttggg gagtaccagt caagagtgat
    18061 ccgaaacatg tggtgtatgt atggatcgat gcattatcca attatattac tgcactagga
    18121 tatggctccg aagatgaaag tctattcgaa aaatactggc ctgcagatgt tcagatggtc
    18181 ggtaaagaaa tcgttcgctt tcatacgatc tattggccaa tcatgctgat ggcccttgat
    18241 ttgcctctac ctaaaaaagt atttgggcat ggctggctat tgatgaaaga cggaaaaatg
    18301 tctaaatcaa aaggaaatgt cgtttatcct gaaatgcttg tggaacgtta tggtttagat
    18361 gcactccgtt attatttgct tcgtgctgtt ccgtttgggt cagatggtgt ctttacgcca
    18421 gaagattttg tttcccgtgt caattatgat ctggcaaatg atctagggaa cttattgaat
    18481 cgaacgattg ctatgatcaa caaatattgt gatggactcg ttcctgacta tgcctcactt
    18541 gtcactccat ttgatagtga actgtctacg acggctgcta acgttgtcgg acgttaccat
    18601 gatgcaatgg aaaaaatgga attcaataca gcattggctg aaatctgggt gctgatttct
    18661 cgtgcaaaca aatacattga tgaaacagaa ccatgggtat tagcaaaaga cgaagaaaag
    18721 aaagctgaat tgaatagtgt catgatccat ttagctgaat ctttgcgtat cgcagcgata
    18781 ttacttcagc caatcatgac agaaacgcca gagaagatct tcaatcaact aggattggac
    18841 tctgaaacaa tgaatcttga aggattacac tttggcgaat ttccttcagg aacaaaagta
    18901 gttgcaaaag gtacaccaat cttcccacgt ttagatatgg aagaagaagt tgcttttatt
    18961 caagaaaaaa tgtctgaagg tactcaaaca aacgaagata cagtcaaatg ggatccagaa
    19021 gaaacagaac ttgtttcaac gaaagaaaaa caaatcaaat ttgatgtatt tgaaaaagta
    19081 gaactaaaag tagcagaagt catcaactgt caaaaagtag aaggggcaga taagttgctg
    19141 caattccgac tagatgctgg agacagccaa gaccgtcaaa tcttgtcagg aatcgctgaa
    19201 ttttatcctg atccaagcga attgatcggg aaaaaagtcg tcattgtagc aaatctgaaa
    19261 ccacgaaaga tgcgtggaca aatcagtcaa ggaatgatcc tttctgcaga agcaccagat
    19321 ggctctttac aagtaatcga agcaccaaaa tccatgccaa atggctcaga aatcgcatag
    19381 aaattcaaag ggggtatgac actagttgtg tcataccccc tttttccgaa atttgagaag
    19441 caattttcgt aaattccttc ttattacttg aagccaatca tataatacag aaacagagaa
    19501 ctatccaaaa taaatcctag aaaaagaagt acgagcttcg ctgatttttg ttaatttaga
    19561 ataatactaa taggaatcgt aaaaacagaa aaaaagcata atgataaagg gtcgggcaga
    19621 ttttaattga ttcatctggt actctttttt attgtcctta cattcataaa gtttgaatgc
    19681 aatgacttta ttacatctat atttatatct aacagtagcg ttgatgtgtt aattataagt
    19741 tctatcactt tatgttcaat tagtttgtgc tcttatatgt gtgattgaaa atatagataa
    19801 aaaaacaatc atcggctttg acttcgatga ttgttttctt tattttgtaa aaatcctatc
    19861 acttgatttt atggtaacct tttatttttg tcttcaaacg gcttggtaag agagcagtaa
    19921 gcagtgcaaa tgggatcgaa aagacatacc agaaaatcgt acgtccgata ccataatgtt
    19981 gatagagctt tgtcaacatc acgtgtaaca agatcaaccc taaaagcaag gagccagcta
    20041 taaaccaatg attatcccaa tgcatgactt cactaagtgc catcactcca aaaggataga
    20101 aataaaaata gatcccgact aaggtcatcc gccagttctt tgtaaatgtt ccgatattca
    20161 ataaaaagaa aatcgctgaa agcaaaatac cgataggcgg aaaaagccca accaaaaagg
    20221 aatacaacat cccccacaag atcatcacag gacctttgat caatgcaacg attgcgatga
    20281 acaaagtcag caatattagt ttttgtgata acgttgcaac agtcattaat ccaaataaaa
    20341 ttaatgtgaa ccaatgccaa tgggactgct gttgttcttt acgggaaaaa tcgacccggt
    20401 tggataaata atctgctaat ttcggataag aacgcttaaa atccataaac tgttcctcct
    20461 tcgtggaaaa ggtaggttta gtatacgaaa aataaaaaag aaatacatcc gtcttaagag
    20521 ggagtttttc actgaaagtg gagaaaaaag aatggtaaat taaaatattc tcagttatac
    20581 tgaaaattat accaaatttc aaagaagaaa gaggatggga atgaaaggca aactaaaacg
    20641 agaaatcaat ctgtttggcg cattagctac cgttatggga accgtgatcg gtgcaggcgt
    20701 cttcttcaaa actgctgctg ttactgctag tactcagtct acaagtttga cattgctagc
    20761 ttggcttttg ggtggtttct taacgatctg tgctggattg acagtggctg aattagctac
    20821 agcaatcccg gaaactggtg gagctgtaaa atatattgaa gctgcgtatg gaaaattgcc
    20881 cagcttttta ttaggatggg cgcaaagctt gatttacttt cctgctaata ttgctgcatt
    20941 atcgattatc tttgcgacac aattgacgaa cttattacaa ttatctaccg attatctttt
    21001 gttgattgca gtgatcaccg ctgtttctgt gacagggttg aatttgttag ggacaaaagt
    21061 cggtgcttct gtacaatcag tgacgttgat tgttaaatta ataccaattg ctgtgattgt
    21121 catatgggga ttattgaccc caggacaagg aactgtccag ttatttccaa tcgaagctgg
    21181 aaaagatgtt acttttgtcg aaggattgag cagtgcgttg ttagcaacgt tgtttgctta
    21241 tgacggctgg ttaggtgtcg gtgcaatggc aggggaaatg aaacggccgg aaaaagattt
    21301 gcctaaagca attattcttg gtttgagttt tgtaacagtc gtttatctat tgatcaattt
    21361 tgtttttttg aaaactttgc cgattgatca tcttgccggt aatttgaatg cagcttctga
    21421 agcatcagac gtcatttttg gaggaatcgg tggaaagctg gtcactattg ggatcttgat
    21481 ctcggtatac ggtgcattga acggttatac actaactggg atccgcgtgc cttatgcgat
    21541 ggctttagaa gacgagctac ctttcagtaa acagctgaca aagctttcaa agaagtttac
    21601 ggttccttat gttcctgcgg ttttccagtt agcagtagct tgtattatga tgagccttgg
    21661 ttctttcgac tttttaacag acatgttgat ttttgtgatg tggctattca gtttactgat
    21721 ttttatcggt gtctttattt taagaaaaaa agcgccggaa ttgccacgtc catataaagt
    21781 accactttat ccaatcgttc cgattattgc gattctcggt gctattttca ttttaggtat
    21841 gacgatgatg acgcaaacga aattagcatt gatcggtata ggtgttacct tgataggtat
    21901 cccagtctat tatcaaaaaa agaaaaaacg ctcagaagag taaaatattt caacaacaga
    21961 cagaaaaagt acgaactagg tcctctagat cttcgtgctt tttctatttc aataaaacaa
    22021 gttcttctat tttgattttt gctacttctg caagtagtgc ttatgtgcta aagtaagaat
    22081 gaaaaggaga gataaacatg atatttgatt ctcataccca tttaaatgct gaacagttca
    22141 atgaagatat tccagaaacg attcagcgag cacaagaaat gggggtaacg aaaatggcgg
    22201 tcgtgggctt cgatacgcca acgattgaaa agtctttgca attaagccat gactatccaa
    22261 atatatacag tattatcggc tggcatccga cagaggctgg cagttatacg aaagaaatcg
    22321 aaaataaatt acaagaacaa ttgactttac caaaggttgt agcattaggg gaaattggct
    22381 tggattatca ttggatggaa gacccgaaag aagtccaaga taaagtattt cgcagacaga
    22441 ttgctatcgc aaaagaaatg aacctaccaa tcagtattca tactagagaa gcacttgaag
    22501 acacgtatca aatcctaaaa gaagaagatg ttcgagatat cggtggaatc atgcatagtt
    22561 tcagtggtga ttatgaatgg gcaaaacgat ttcttgactt agggatgcat atttcattta
    22621 gcggtgtcgt cactttcaaa aaagcattag acgtccaaga ggctgcagca aatgtaccaa
    22681 tcgaacgatt acttgtggaa acagatgccc cttatctggc accagttcct tttagaggaa
    22741 agcgaaatga accaggctat actcgttata cagtagaaaa gattgctgaa cttcggaatc
    22801 ttccgacaga agaagtggct gcccaaacat ggacaaatgc gcaccgatta tttggtctaa
    22861 gcgaacatgg ataagatgaa gatccaagag attatagtcg ttgaaggaaa agacgataca
    22921 agaaggctgc gggaagtagt tgatgcagat acgatagaaa caatagggtc tgcaatcaat
    22981 gaagagatcc tgatgcaaat tgaacatgcc caagagactc gtggagtgat cgtttttacc
    23041 gatcctgatt tttcaggaga aaaaataaga aaaatcatta tggaagctgt tcctcaggca
    23101 aaacatgctt ttttgccgcg cacacaagct aaaccaaaga aaaagggaag ccttggtgtc
    23161 gagcatgcaa gtgatgctgc tattttagaa gcattgaaaa aggtcgtgac gccagacatt
    23221 tcccaaacgg ctgtttctga aatcactaga caagaccttt tagactatgg tttaatagct
    23281 ggggcacaag caaaagaaaa acgagaaaaa ctaggtgacg agttgcgcat cggttacacg
    23341 aacggcaaac aattggaaaa acgtttgaag atgtttcgta ttacgccgaa agaactcgcc
    23401 caagcaatga aaatagtgga ggaacaacat gagtgaatat cgtgaaattg cgacaccctc
    23461 tagaacaaaa gagattttat tgaaacatgg tttttcattt aaaaaaagtt taggacaaaa
    23521 ttttctgact gaaccaaata ttttaaggaa aatcgtagaa actgcgcaaa tagatgaaca
    23581 tacgaacgtg atcgaagttg gaccaggaat cggtgcattg acggaacaat tagccaagca
    23641 tgcaaaacaa gtcgtagcat ttgagatcga tgatcgtttg atccctgtat tggctgatac
    23701 gatgcaaccc tatgacaatg tgactattgt acatcaagat attttaaaga ccgatttgtc
    23761 aacagctgtt agagaaacat ttcatgagga gcttccgtta aaagtcgtag cgaatttacc
    23821 ttactatatt acgacaccga tcatgatgca ttttcttgaa tcagatttgg ttgtggatga
    23881 actagtcgtc atgatgcaaa aagaagtagc tgatcgtatc tctgctgaac caggaacgaa
    23941 agcttatggt tcattatcga ttgctgttca atattatatg gaagccagtt tagcattcat
    24001 cgtaccaaaa acagtatttg tccctcagcc aaatgtagat tcagcgattt tgaaactgac
    24061 aagaagagat ataccggcag tcgaagtgac agacgaaaaa gcatttttcc gattgacaaa
    24121 agcggcattt cagcaaagaa gaaaaacgct ttggaataac ttgcagcatt cgtatggcaa
    24181 agatgaccag acaaaagcat ggttagcaaa aagcttggaa acagctggaa tcgatccaaa
    24241 acgtcgtggg gagactttgt cattacaaga attcgccgca ttaagcaatg cgatgtcaga
    24301 aaatatgcag taaaaaaaga ggttgtgaca gaagttgtga aaaagctgtc ctgcatcaag
    24361 taataagaac agccaaacaa aaatagttcc tcatgttttt cgtttgtttg ggcttgttac
    24421 cgcagatgca agcttttgaa caccgtttaa gaaagaggtt gtgggcctgc cgtttaactc
    24481 taagtcacaa ggaacaattt caaaaaacag cttctcatgt ttttgagtta gttggtctta
    24541 ttacttgatg caagcttttg accactatta tgccgaacaa ggagatgtga cacaaatttt
    24601 gtcacatctc ctatttttgt ttcttgatca aaaaattctt atacttgata ctcacactca
    24661 ttttgtatgg atttagaaaa gaatatgtgt cttgacagtt attagtaagt gagttatact
    24721 aagtcgacat ttatgtgtca agacatatta ggagagagaa aaaatgagaa aaaattcagt
    24781 tcgaaaacta acgatttctg ctttattgat tgggatgggg gtcattatcc caatggtcat
    24841 gcctaaaatc atgattccac cagcttcctt tactttggcg tctcatgtgc cattatttat
    24901 tgcgatgttt tttacaccag gagttgcagt ggctgttgcg ctaggtacga catttggttt
    24961 ctttttaaca acgccggtta tcatcgcatt gcgagcattg tcacatcttg tgtttgcgtt
    25021 gattggggct tggtacttgc aaaagcatcc gagtatcgtt ttaaaaaatg gacagtttac
    25081 ttttgctaat tggcgtttcc aagggttcaa ttttgtgatc ggtgtgattc attctttagc
    25141 agaaatgcta gtagtcagca tcttttactt tattggtaat atgcctgaaa catattattc
    25201 tcaaggatat ttttatacag tattcatctt gatggggatt ggtgggctta tccacagttt
    25261 agttgattat aatattgctt attttatagc aggaacactc agcaaatatt ttgatatccc
    25321 cgtatttacg gcagcaaaaa aagaaaatcg gaaagagtcg attcaagggt acgcgaagaa
    25381 aacgacatat tagacatgat ctctcaaaaa aaaaactctc atctagtaat ggataataaa
    25441 tcctctaaga tgagagtttt ttgtcctaaa aagagctgca ccaacatctt ctggcacagc
    25501 tctaacaggg ggcaatttat ggaagtttga tgacttgttc tgtttcttta tcaaagaagt
    25561 ggcctttgct gatgttgaag gcaaggtcga ctatttcgcc tggtttatgg aaatctcttg
    25621 catctacttt tgagatgaac tcagtagaac cagtttttgt gtaaagcatc gtttcagctc
    25681 caagtaattc ggaaacgaca acttctgatt tcactacggc ttcaggtgaa gcttcaaggg
    25741 ctacttgttc actgtggatg tcttctggac gaataccgaa gataacttct tttccttcat
    25801 aacctttttc aactaatact ttgttttttc cttcaggaat acgaagctta agcccatgtc
    25861 catctgtgat aacaccattt ttcaatgtca cattaaagaa gttcatggca ggagatccaa
    25921 taaagccggc aacgaacata ttgacgggtg tatcataaac ttctttcggt gtaccgattt
    25981 gttggataaa tccgtctttc atgataacga ttcggtcagc catagtcatt gcttctgtct
    26041 gatcgtgagt aacatagatg gtcgttgttt caagacgctg gtgaagtttg gcaatctcag
    26101 cacgcatggc tacacgtaat ttagcatcta aatttgataa tggctcatcc attaagaaga
    26161 cttttgcatc ccgaacgatt gcccgtccca atgccacacg ttggcgttgt ccaccagata
    26221 aagcagctgg ttttcgtttc aagtattctg tcagacctaa gatctctgct gcattgtcca
    26281 cacgtttttt gatgtctgct ttatcatatt tacgtaattt caagccaaaa gccatattat
    26341 caaaaacagt catatgagga tacaatgcgt agttttggaa aaccatggca atatcacggt
    26401 ctttaggtgc cacgtcattc atgacttttc cgccgatatt tagttctcct tcagagatat
    26461 cttccagtcc cgcgatcatt cgcaaagtag ttgactttcc acaaccggaa ggaccaacaa
    26521 aaacgataaa ttcacggtct ttgattgata gattaaaatc agttacagaa taattttctg
    26581 cattgtcata ttttttatat acgtttttca gagccatttc taccataata cgttccacac
    26641 tttctttttt agttttatta actatttaaa gtataaatga aagcgttaac taacttcaat
    26701 gtcatgttga acaagaattt tttctttttt ttgtcaatgt gctagtttta agctttaata
    26761 aatatccata agaaataaaa actgatacac tggagtaaat aagtatgtta acaatatcaa
    26821 aaggagtgtc tcttatgaaa atcgctttga tcgctcatga ccgaaaaaaa cctatgatgg
    26881 tgaaattagc aacagcgtat aaaacaatat tggaaaaaca tgaacttttt gctaccggga
    26941 cgactgggca gaggatcacg gaagcaaccg gcttgccgat ccatcggttt aaatccggac
    27001 cgttaggcgg ggatcagcaa attggagcca tgatttcgga ggatcagtta gatttggtga
    27061 tttttcttcg agacccgctg gctgcacagc cccatgaacc agatgtaaca gcacttatcc
    27121 gtttatctga tgtctatgag attccgttag ctacgaatat cggaacagct gaggtgctac
    27181 taagaggact ggaagccggc ttcctagatt ttcgaagaat cgtccatgaa atagacaagc
    27241 atccactttc attttgatgg ataagcgtat ggaaaaataa cgtttaatgt tcgcctatat
    27301 gtagctataa aagctaaaca aactgataat atgcgatata atacgaacgt attggataat
    27361 ataaacaata atattagttt ttcgtatttg cagtaaatgt gaaaatatgc tatagtttct
    27421 gtgggaaaag atcccaaaaa taatacagag tagaaaataa agcgttaagt gctaagagga
    27481 tgggatgttg cctcttggac gaaggacgta atgttcgcgg ttttattttc gcattcgctg
    27541 catgatacga tgtagcagct cttaaaggag atgctaaaaa tgaagaaaca ccggttagac
    27601 gcacgtatga ttgctattat gggcttattg attgcattga tggtcacttt gtcccgactg
    27661 gtagcaatcg agaccccatt tattaaaatc agtgtcacat ttattccaca agtgatcatg
    27721 ggcattcttt ttggtccttt ttggtcaggg ataggcgcag ttttagctga tttagtagga
    27781 atggccctct tctcgaaatc tgcattcttt attggattta cattgaatgc atttattgaa
    27841 ggagcaatct acggattctt tttctatcga aaagagatca cttggaaaaa tgccattctg
    27901 gctacgctta gtgtcacgct gatcatcaac ttatttttga caccattatg gcttgcgttg
    27961 atgtaccatg tgccattgtt cagctgggtc gtttgggcgc cccgcctgtt aaaaacagta
    28021 atatggttac cgattcaatc aattgccatt tattacgtag gacgtagtat tccttacaaa
    28081 aaaattttaa gaagtttagc gatccacgct aaataaaaaa caagcagtct tcccatccca
    28141 gaagactgct tgtttttatt gtgctgtgaa ttgtgcttga tagagccgtc ggtaataacc
    28201 atctttttga agcagctcat catgggtgcc catttcgaca atagctccct gatccatcac
    28261 taatatctgg tccgcatttt tgattgtgga caggcgatgg gcaatcacaa aactggtttt
    28321 tccttccatc attgctaaga aggcttgttg gatttttttc tctgtcaatg tatcgactga
    28381 gctggtagct tcatctaaga tcaacatagg gggattactg atcatcgttc gagcaatcgt
    28441 caacagttgg cgttgtccat ctgatatctt taacccttga ccgccaatct tagtatctaa
    28501 tttttccggc aaacgttcta caaattcata catataagag gcttttaggg catcattgat
    28561 ttctgcgtca cttgcttccg gattcccaaa acgaagattt tctcgcaatg tactgtcaaa
    28621 tagccaagta tcttgtaaca ccattccaaa acttttacgt aatgtatcac gttggataga
    28681 gcggatgtca tggtcatcga ctgtaattgt tccttttgtt gtgtcataaa aacgcatcag
    28741 caaattaaca agtgtggatt ttccggcccc tgtttttcct actatagcaa tggtttgacc
    28801 tggataagca gtaaaattga aatcttcgat caaacgctgc ttggggtcat aagaaaaaga
    28861 aacatgatca aaagagactt ttcctttgat atttttgggt tccaaggcat aggcatctgg
    28921 caaatcaggc ttttcttctg gctgatcgat caaatcaaat gctcgttcta gaccagaaaa
    28981 agcggtttgc agttgtgtcg tgattccaga aagttcgata aatggtttag aaaactgact
    29041 tgagtagatc gtaaagctgg aaatcacacc aacagtgatt gacgaaccac tcttcaatgc
    29101 caataggccg ccaactaaac caatcgataa ataagcaagg tgatctacaa aacgagaaga
    29161 cgggtttgtc aaggaagaag aaaattgtgc ccgttgtcct ttttcatata gatcagcatt
    29221 tagcagttca aattgctgtt gactagcttc ttcttgctga aaagctttca cgattttctg
    29281 gttaccgatt cgttcggtca caaagcccga gatatcccca ataatccttt gctgtctatc
    29341 gaaatttgtt tgtgaagcac gagcaacgat ccaactcaca aggaaaataa ttggtgtaga
    29401 acaaagaacg accaatgtca gaagtggact tagacgcatc ataaaaataa acgaaagaat
    29461 gatgattgcc actccagaaa ataattgatt gaaggccgca gaaattgcaa tggaaatatt
    29521 gtccatatca tttgtaaatc gactgacgat atttccgtgg gagttttgat cataataacg
    29581 aagtggcaat cgattcagat gagcaaatgc atcttttctt aattttgcaa cggataagta
    29641 agctaaccga ttgcttaaca cttggatgag ccattgactg actacgttga ttaaaacgat
    29701 accaccgaat agaataagta aatggaacag tcgattgaag cggacctgtc cttctccaat
    29761 catttgatcg atactttgac caatttgtaa cgtcatcaca actgttgcaa tcccattgat
    29821 gattcctagt atcacagcta gccaaatgtc ttttgcatag gcagtaaggt aaggaatgaa
    29881 tcggcggagc gtctgcaagg ataatttttt tactttcata actattcttc ctcctgtgat
    29941 gcgacgattt cctgatattc tgctgattga gcaagtaatt cttcatgggt accctgagca
    30001 gcaatcttac cttgatccat cactaagata tgatgtgctt cttgaataga gcggacacgt
    30061 tgggagataa taatcactgt actgccaaga taagtctgta gcgccttacg caaatccaag
    30121 tctgtttgat aatccaacgc acttaaagag tcatctaaaa tcagtaatgc tggcttggca
    30181 atcaaagctc ttgcaatcgt cagtcgctgt ttttgtccgc cagagaaatt tttcccattt
    30241 tccatgactt ttgtatctaa accatttgga agctgacgga caaattcttc tgcctgagcg
    30301 attttcagag cttcccaaca ttctttttct gtggcgtctt tcttacccca ttgaaggttt
    30361 tcacgaatag tcccggtaaa tagtacagaa gtttggggaa caagagcaat ttgctgacgg
    30421 agatgatcca gctgccaaga acgaacatcc attccgtcca ttataactgt accggaactg
    30481 acatcataga aacgcggaat caattgtgtc aaagtcgttt tcccgcttcc ggttggacca
    30541 atgattccaa ggactgtatt tcgcttcagc tgaaatgtaa tattttggag agaaagacca
    30601 gaatcaggag tataacggaa gtcaacatgg tcaaaagcga taattgcttc agaagaaaaa
    30661 tcgggagtag tactgacctc agcttcaaga atcgaatttt ctgtgtctag cacctctttc
    30721 acccgatttc ctgaagcttc ggcacgtgtg aagatcacga cgaggtttga aacaacgatc
    30781 agtgccaaaa gcatttgatt catataattg atcaaagcaa gaacttgtcc ttgttgcaag
    30841 ctaccaatat ttactttgat cccgccaact gtcagcaaac agatgatccc gacattcatg
    30901 atcaaggttg tagcaggtga taatagggca gagatattgg aaacacgtaa ataattgttc
    30961 gctagatcat ttgtgctttt atcgaacttc tctgtttctt tttttgtctg cgcaaatgcg
    31021 cggatgacac gaacaccgct aagattctga ctaacgtgtt cattgagctg atctagtttt
    31081 tcttgcactt tttggtagag aggaacggtt tttttgatga taaaaaataa aatgattgag
    31141 aaaataggca ataatgctaa gaaaaacaga cctacttccc agtcgatcac aaaagccatg
    31201 accactgacc cgatacttaa aaacggtgct ctgatcaata aacgaatgac cattgctaaa
    31261 gctagctgaa gctgattgat gtcgtttgtc attcgggtga tcaaggtatc tgttccaaaa
    31321 tgattcaact cgttgtgaga gaaagtattg atttttttga ttagctgatt acgtaactct
    31381 gtaccaaatc cttgtgaagc tactgaagca tagtattggc aaatgatagc acaaatcaga
    31441 ccaatcacag acatagcgac catccataag cccatttcaa tgatttttcc tcggtcattc
    31501 atttttagtc cattatcgat caaggaagcc atcattaaag gcaggacaag ttcgaagaca
    31561 gcttcaagaa atttgaagaa cgggcccaaa atgatttgct tacgatagtc ttttgcataa
    31621 cgtaataact gaatcatatg attcctccta ttatttattt gtccacaatt gaaaaaacaa
    31681 gtactaccac attgtagctt aggaaattta gaatgaaaag agaacaagcc ttttcataaa
    31741 tgaaagtttt attatgaaga agctttagaa attgaataga ctattttttt taaaaaaaag
    31801 aaaatgagaa aacatgccag tctcttcatt cataggaacg tctattttac ttaatttttc
    31861 tataaatgaa ttctgcataa aaaatatatc aagtaaatgt ttatagatga aactctattt
    31921 acttcttttt taatttgttc ttgcaatctt ttttgctacg taatttttgt aaaaaagtgg
    31981 gttgagagaa atctaattga ttttcaaaga aggagtatga aaatgagaaa aatatttatt
    32041 cgtttattgt gggtactatc ttagaggaat aaataatagt ggagaaaact atggtttggc
    32101 gcagattcta tcaggtttga tggaacgaag acaacctccc gacatcaagt caaaagctac
    32161 ttgagatttt gatctgaaaa taaaaaaaga aaacaaggta ggaacatcaa tgtttctacc
    32221 ttgttttcgt aatcaatggg aaaaaactaa gagtggtgta cgtaatcaat aaaaaggatg
    32281 gtgacaacag tacctcttta ctctctgaaa gcatcataac tcgtttttcc tcattatgcc
    32341 aataaattgt tgttatacca catataagga tttgtaatag ctaaaaacag acaggaaagt
    32401 tgaaatctgt gaatcttatt ttgtatggaa ggatttatag aacgcaaaaa tcaagataaa
    32461 gatgaataaa aatcctgttt cggctatata ttgcgtatct acgccaataa aacgagtttg
    32521 ttctgtcata gccatataaa gcaaaggcaa aaatacaact acagcgagat aagatccgat
    32581 gatttctcga aaagttttcc aggaagtatg tttcctgttt ttatatttat ccgatttcat
    32641 tgaatagtca tagcctcctt attctttcat atgttgaaat tccagtataa aaactggaag
    32701 gaactgtacg ttttttagaa taaaaattat aagggataag taggctgctg tctataataa
    32761 gatatatttt tcaattcttt attaaagatt ttattgacaa aatgaatccg ttttctatga
    32821 tggaggaggg gtgaataagt tgttgaagaa ggaaatttta tgttacttag agaaccagac
    32881 agcctttttc gatttagaac atatgaacga ggtctttaca gcacaaaggt tagctgatca
    32941 ttttggggta aaaagaaaca cagtcagtca ttatttgaac cagttaaatg atgagcaact
    33001 actagtcaaa atcaattctc gaccagtgat ttatttccac aaagaagctt ttgaaaaaca
    33061 attttttaag ttacgcacga atttttattt ttctatcgat gatctcaaag aggaacagcc
    33121 ttttttcgcg caaccaaaag acttattctc ttttatgatc ggatacgacg ccagtttgaa
    33181 agaaagtatc gaacaaatca aaacagctct ttactatcca gatggcggat taccactttt
    33241 gattacaggc gaaagtggca caggtaaaag ttatttagtt aatttggtct atcagtattg
    33301 tctgcttcat gatctgctag aagattctgc accattcatc acttttaatt gtgcacaata
    33361 tgcagataat cctgaacttt tgactagcaa tttgtttgga catgttaagg gagcgtacac
    33421 tggtgcagaa gaaaataaaa aaggggcttt tgaagcagca gatggtggga tcttgttttt
    33481 agacgaggta catcgcttaa caccagaagg tcaggaaaaa ctgttcacct atcttgatca
    33541 aggtgtgatt tatcgtatgg gagaacctaa tttgtcacgc cggatcaaaa cacgtctctt
    33601 ttttgctacg actgaagaga ttactagtca tttcttaaca actttcattc gaagaatacc
    33661 tatccgaatc gaactacctg cacttaatca gcgtagtcgt aatgaacggc tagaactgat
    33721 ttactctttt tttctcgaag aacaaagaaa aatggggagc cctttgtcag tcagtggacc
    33781 agctttgtca cttttaacaa gtcagaaatt atccggaaac ataggagagc tgaaaaatat
    33841 cgtcaaagtt tcagcagcaa aagcatatgc tgaacagcgt gaacagcctg aaatccatgt
    33901 cacgatccat catttgcaaa aagaactact tgcgcagcct attactaaaa atacagcaca
    33961 gcaaggaatc tatattacgc aagaccagac cttagagcaa ttaatggaga agcaacagcc
    34021 cgaacagcag cgaatcgtta aaagttttga gcaaatccta attgatttca agaaaaataa
    34081 ctgtctgcta tattcttgcg aaggaaaatt aaagcaagaa gtgattcaat tatttgattt
    34141 tttgctgttt gagaccagta gacaagaaaa acacgagctg ctggtttatt ggacacaatc
    34201 tatccgagag acctttcgac aaatggaaac tgcctatcaa atcaaattcg atggaaacag
    34261 tgtctatgcc ttgagttact atctctatca gcgaagcact atcaaatggt taccggaaga
    34321 acatgatatt atccaactaa tcaaagaatt agaacaacaa gtttctcagt cttatccatc
    34381 tagttatcac tatgtccaac gaattctgga attaagtaag atcaaattag atattgaaga
    34441 cacaagtatg gatcggattt tactgacgat ctatctaaaa aaagcgaaat ggtcaaaaga
    34501 aaaacgactg ccaaaagcga tgattgctgc tcacggttat gcaactgcta gcagtatggc
    34561 aaatgtagta aatcgtttat tacaagagga tctatttgaa tcatttgata tgccactaga
    34621 tgtcacacca cagcagattg cttcagaaat ccttgattat tgcgaaaaca gcgatgtttc
    34681 aaatggattg atcatattag tagacatggg atctctgaaa gaaatccatc agttttttaa
    34741 aaaacagtta tccgtaccgc ttttgatttt gaataatgtg acgacacctt tagcaatcac
    34801 agtaggggaa tgcctgcaaa aaaatgtatc attagaagag atagcagaag agactgttcg
    34861 acagatccag ccagaatggc gactgctcta tccagaggaa aacaaaccca aagcactcat
    34921 tactacttgc ttcactggaa tcggtacggc ggtccactta agtgaactgc tagaaaaaag
    34981 cttacctacg gcttgtcagt tgaaaatcat cccatatgaa tatcagcaat taaaagataa
    35041 gaaaaacagt gacccattat tttccatata cgaagtcgta ggaatgattg gcaccacaga
    35101 tcctgatatc accaatatcg actatctttc attagaagac ctcatctcgg gggaaaagat
    35161 gtctgtacta gctgaatggt tggacactac tatgaatgct tcagagaagg aaatattcaa
    35221 tcagcgcatc attcgtaatt tttcattgga aaaagtactg gattccgtga ctattctaga
    35281 tacagagaag gtgatgggag aaatcgatca ttttatgaga gaattagagg tgcagttgaa
    35341 tcgaagcatc agcaatccca aaaaattagc gttgtatgtc catgtgagct gtctcattga
    35401 acgattgatc cgacaaatgc caatcgaaac ctatcaagga ttagagaaat ggaagcagtg
    35461 tcaaaaagaa gggttatctg ctattaaatc tgcgtttagt gtcattgaag agcattatag
    35521 tgtcatgatc cccaattctg agattgccta tatctatgat attttgagtg aagatacaga
    35581 gtttctctcg atagaagaag aattttgaac ataagcaagt tgttgccact aattttggca
    35641 tgcaacttgc ttttttagta tgtgtaagtg aaaagaggag atgaaaaagt ggatagagaa
    35701 attatcttag cttcccatgg aaaatttgct tcagggatat tagattcatt agagctgatc
    35761 tatgggaaaa atcattctat tactgtattc gattgctata ctgatgagaa ttttgattta
    35821 gcggaaacag taaggcagct ttttatgagg tacaaagata aagaaatcat tgttctgacg
    35881 gatctttttg gcggaagtgt caacaacgaa ttcttacaat atatcaatcg ggatcatgtc
    35941 tatctcgttg ccggattgaa tttgccacta ttgattgagc tagtatctcg gttggacgct
    36001 gaagtagaga caactgtttt gatccagcag gtgctaactg attcaaaaaa aatgatccag
    36061 ttttgtaacg agcggctcaa tcagaaaata gaagaagacg aattttagga ggaagaaaaa
    36121 tgattgtatt gacaagagtc gaccatcggt tattacacgg acaagtagct ttttcatgga
    36181 cacaaacaat aggcgcagac tgtattttga ttgctaatga tgatgtacct acgaatgaga
    36241 tacgaaagac aacgatcaaa ttagccaagc cacaaggagt caaactagtg atcaaatcaa
    36301 ttgatgactc gattgccgcg ttgaaaagcg gggtgacaga taaatataag ttattcatcg
    36361 tagtggaatc gatcgaggat gcatataagc tggcagaagc gtatccagaa atcaaaacca
    36421 tcaacttagg agggatcaag gcaaaacctg gtactcgcag catcggaaaa gcggtaaata
    36481 ttttaccaga agaggaggaa cgcttgaaac gattaattga caaacaaata gaagtagaaa
    36541 tccgtcaagt accagcagat aaaaaagtgg atgtaagaga agttttgtaa gaaaggaaga
    36601 acctcaatga ttgtacaagc agtcttatta ggaattgttg catttgtcgc acaatcagaa
    36661 tatgcactag ggacatcgtt gctttctcgt ccgattgtga cggggctgtt tacaggaatc
    36721 gtgttaggtg atgtaaaaac tgggatcatt atgggcgcaa ctttagagtt agcatttatc
    36781 ggatcgttct cagtaggtgc ttcgattcca cctgatgttg tgaccggtgg gattttaggg
    36841 acggcttttg ctattacagc tggagccggt acagaaaccg cgcttttact aggtttgccg
    36901 attgctacgt tgacattgat attgaaaaat atttacttag ggcttttgat tccaattatg
    36961 aaccataaag cagacaatta tgcagaagaa ggaaattata aaggagtcga gcatatgcac
    37021 ttgcttgccg gtatcggtct ttccttgatg cttggtgttg tggtaatggt ttcttatatg
    37081 gtgggaagca atacgatcgg aaatctatta aatatgattc ctgacttcgt tcaaaagggg
    37141 ttgtcagtag ctacagggtt gattccagct cttggttttg cgatgcttgc tcgtttgctg
    37201 atcaacaaac aagttgcacc atattttttc ttaggttttg ctatagcttc ttacttagag
    37261 attcctgtga ccggagtagc tatttttggt gcgattttag cagttgtggt agtcaatatt
    37321 atgaatgtcc gtcaactacc acttcaaaca actgcagggg aggtagaaga agatgaagaa
    37381 ttctgagatc aaaaacagca aaataacgaa aaaagagcta aatcacgtat tttggcgttc
    37441 ttttcaaatg gagttttctt ggaattatga acgtcagatg aatatggctt atgtttatgc
    37501 aatgattccg attttgaaaa agttatatca gtcgaaagaa gaaatggcag cagctttgaa
    37561 acggcatcta gagtttttta acacgacccc acatatcgtt acactgattt taggaatcac
    37621 ggctgcaatg gaggaagaaa ataaggatga tcctgaattt gatgtgactg cgatcgatag
    37681 tatcaaaaca tctttgatgg gacctcttgc agggttagga gattctttct tttggggaac
    37741 tttgcgattg attgccacag gtgtgggtac ctctttagcc atgcaaggaa atattttagg
    37801 ccctatcttg ttcattttga ttttcaatat tccgcacatt ctgtttcgct atctagcaac
    37861 aggttgggga tatcgcttag gaacgggttt tttgaaaaag atccaggcaa acggtatgat
    37921 ggaaagtttg acattaggcg cgtctattat tggactgatg gtcgttggag caatgactgc
    37981 cacgatgatc accatcaata ttcctttaaa aatcggttca ggagaaaatg ctgtgactgt
    38041 tcaaagtatt tttgatgata tcgttccaaa tatcttatgt cttggagcat ttggcatagt
    38101 gttttatcta ttgaagaaag aagtgaaacc attaacgatc ttgatcggac tagctttctt
    38161 tggaattttt ggttcgtgga tagggatttt ttaaaaaagg agcagagaaa atgtttacga
    38221 tgcaagatta tatctatgaa gaaaaggaag tactaagtac gatcttgaaa aagaatgatt
    38281 tttcgacaag agaacatatg aaaaaaacag tgaatttact gatcttagca acgggttcat
    38341 cttataacgc ttgtttagca gcgaagccag cattggaatc ttatggtgat cttactgtcg
    38401 atattcaaga acctttttat tttttcaatt atggaaaact ttctccttca atcgatactg
    38461 tgattgctgt gtcacaaagc gggaaaagtg cttctactgt tgaagcagta aaaatgatac
    38521 aaaaacaagg gttacctgtt gttgctatca caaatgacgt gcaaagtcca cttgctttag
    38581 aagctgcaca aattattgat ttaggtgccg gggtagaatc agtaggcttc gttacaaaag
    38641 gctattctgc gactgtttta cagttactac tcttagggat aggaattgga ataagtaaaa
    38701 ataaaatcag caaaaaaata gaacaagact acatgcaaca gttgcgcaaa ataatcaacc
    38761 atttaccagc gattattcaa aaaacggaag aattttttga tggatatcaa agtttatttc
    38821 gtttagcaca gcgtttcgtc accattggat atggtccaaa ctggggaaca gctaaagaag
    38881 cagagacgaa attgactgaa acaatccgcg ttccgtcaca aggatttgag ttagaagctt
    38941 atatgcacgg cccatacctg gaggcagatg cttcgcattt gctattcttt attgaagggg
    39001 aaagtgtaaa taaggaacgt tcgcaaaagt tgcaaagata tatgagtcgg tacgttggag
    39061 agactctcac cattaccaca aaaaaagcaa gaaacgagaa aacacttgga ttagcaatag
    39121 aatgtgatga atatctgtct gtattagcgc ttgtcgttcc gtttcaacta ttcgcttata
    39181 aaaccgcagt agctaaaggt attgacttga acaaaaagat ttttgaagac tttgatacag
    39241 tcttgaaaag taaattataa gaggtggaaa tcatgctgaa attcaatgaa gaagaacaaa
    39301 tcaaagcaat aaaaggcgca ctagctttac gtccgcaagt agaagaaatc atcgataaac
    39361 tttatgcgga aaaatttgac gctgtctatt atctaggaat cggtggaacg tatgcttcct
    39421 cgatgcaagc tgtcacttat atgaatggga aaagcaactt gcctgtcttt gtacagcatg
    39481 ctgcagaata ttacactact ggaaacaaaa gattaacaaa agattctatc gttattttgt
    39541 cctctgttac cggtacaact caagaagtag tgaaagcagt agaacaaatc aagaaagtag
    39601 gagcgacact gattggattt atcgataaag cgaacagtaa gttgtctcag ctatgcgatt
    39661 ttgtggtcac ttatccagct cctggaacag aacaaatcaa attttttatg gcagcagatc
    39721 gattgatgta tttgaatgga gaattcgaag cttattcaga atactatgaa cagctcgaac
    39781 gttatttacc aacagggtta gtggaagcag agaaaaaagc agatgcattt ggcttatcat
    39841 ttgcagaaaa acatcgacgt gatagtatgc attattttat tggagcaggg aatcaatggg
    39901 gagctgttta ttcatatgcc atgtgttact gggaagaaca aagctggctt ccatcgaaat
    39961 ctatccatgc tgccgagttt cttcacggga cgttagagat cgtagaagaa acaaccccag
    40021 tcacattatt tttaggcgaa gatgaacaga gagtgttatc tgaacgtgtg gctaaacttt
    40081 tgccaaaaat ttgcagcaat tatacattga tagacacaaa agactatcca gtagaaggta
    40141 tcagtgaaaa atatcgcgga cgtgttttgt ctttcttatt gatgcatgta gtgacacaac
    40201 gcatcgatgc tcatgtggag cagttgaact gccatccatt agagatacgc cgctactatc
    40261 gtcagtttga ttattaggat agactggata aaaagaatat aaaaaataaa gagagcagcg
    40321 ataactgctc cctttatttt tttaaaatat tggcatcaat tacgttataa aacacatttt
    40381 ttgcattatc tacagttcaa cttataatct gtttaatggg acatgtgggt gccaaccatt
    40441 ttttgttatg caatagtact atttagttgt tgcatggatc cgtagtaaaa gtccaagtac
    40501 aacgagttgc gccagtttac atagcgtttt gtatccagaa tgttgactaa aacaaaagtt
    40561 cctatgtcaa ccgtcaacag agacaatctg tccgttgcag gacctgacag atttagaaat
    40621 gttcaatctg tctcttatgt attttaaatc aatactcact aggagaagtt tcctaacctc
    40681 ctaatagtaa tgctggtagt aacattgtca aaactgcttc ctcttaggaa caagcaaagc
    40741 ataacgagtg taaaaataaa atgtagacta ttgtaacata tcattgatta tttcaagtaa
    40801 aaacgataaa agaagtaaac aaacacttaa aaacaatgga aattagtccc aactttaaga
    40861 aatgtatact ataatggaaa ttatcaatta tttatttgga ggaatatcca tgaaacaaaa
    40921 aatcgcagtt cttggtcctg gatcttgggg cactgcttta gcacaaacat tggcagaaaa
    40981 cgggcatgat gtccgtattt ggggaaatgt acctgaacag attgatgaaa tcaataagta
    41041 tcatacaaat cagcattttt tacctgattt gacgatccca gaatcgatta tcggatataa
    41101 agaattggca gaagcagtgg ataacgcaga tgctatttta tttgttgttc caacaaaagc
    41161 gattcgttca gtcgcacagg aacttattgg taaaatgaat actaagccaa tcattataca
    41221 tgcaagtaaa ggactagaac aagatacgca taaacgaatc tcagaagttt tggctgaaga
    41281 aattccagaa gaaaaaagac aggcaattgt tgtattatct ggtcctagcc atgctgagga
    41341 agtggcagta catgatatca caacaattac agctgcaagt attgatcaaa aagcagcagc
    41401 ctatgtacaa aaactgttca tgaatgatta tttcaggatt tatacaaata atgatgtgat
    41461 tggcgtagaa actggtgcag cactgaaaaa tatcattgca attggtgccg gcgctatcca
    41521 tggattagga ttcggagatg atgcaaaagc agcaatcatg actcgaggat tggcagaaat
    41581 cagccgtctt ggtgtcgcga tgggtgccaa tcctctgaca tttatcggtt tgagtggtgt
    41641 gggggacttg atcgttactt gtacaagtgt tcattctcgc aattggcgtg ccggtaactt
    41701 gttaggacag ggacataagc tggaagaagt tctagaaaac atgggaatgg tcgttgaggg
    41761 tgtggcaacg acaaaagcag cagtagagct agctcaacag ctaggtgttg agatgccaat
    41821 tactcaaaca atctataatg ttttgtataa tggagaagat atcaaaaaag cagcaaaaga
    41881 aatcatgtta cgtgacggaa aaatggagaa tgaattttct cttcgttaaa tagatgatca
    41941 atagttaatc gtcgtttagt ctaatggtga taagtcaaga tgaaatttaa gattttcttt
    42001 attgagt
//
LOCUS       DACEEZ010000015        39413 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20313_2017
            SAMN12639966-rid9879223.denovo.015, whole genome shotgun sequence.
ACCESSION   DACEEZ010000015 DACEEZ010000000
VERSION     DACEEZ010000015.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639966
            Sequence Read Archive: SRR10023158
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 39413)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 39413)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741683.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741683.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:44:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,016
            CDSs (total)                      :: 2,962
            Genes (coding)                    :: 2,889
            CDSs (with protein)               :: 2,889
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 36 of 73
            Pseudo Genes (incomplete)         :: 29 of 73
            Pseudo Genes (internal stop)      :: 18 of 73
            Pseudo Genes (multiple problems)  :: 8 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..39413
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639966-rid9879223.denovo.015"
                     /isolate="E20313_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            63..380
                     /locus_tag="IWO89_000876"
     CDS             63..380
                     /locus_tag="IWO89_000876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0455649.1"
                     /translation="MKKVHSFLNYLMGAITIVAVYQAFLKYPDYGTKLLSLTHNQTAY
                     SPTLILSTLTAGAKSATYFFHKTTSMLKRKKQRKQLLVVVAAMIIIYFLPVITQVFHQ
                     YFG"
     gene            540..776
                     /locus_tag="IWO89_000877"
     CDS             540..776
                     /locus_tag="IWO89_000877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HAQ0455650.1"
                     /translation="MKKMKKYKIRKSGNSDVTTIPPEVKEFMGVQTGDAISYVFQSDG
                     SVRMIKAQEEPDIDSLVDSIMNQYEDALKDLVDL"
     gene            773..1171
                     /locus_tag="IWO89_000878"
     CDS             773..1171
                     /locus_tag="IWO89_000878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system death-on-curing
                     family toxin"
                     /protein_id="HAQ0455651.1"
                     /translation="MRYLSTKEIIKINAKVILRHSPGEMIGVKDANALDMAVKQPSQA
                     VFNQELYPEIYEKAAILAINLAKKHPFHNGNKRTALVAMLLFLQLNNCPVAFSRQEAV
                     DFIIMITTSSLDFDSLKSKITSYLRQTAIK"
     gene            complement(1838..2782)
                     /locus_tag="IWO89_000879"
     CDS             complement(1838..2782)
                     /locus_tag="IWO89_000879"
                     /EC_number="1.1.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate dehydrogenase"
                     /protein_id="HAQ0455652.1"
                     /translation="MKKTSRKVVIVGTGFVGTSIAYAMINQGISNELVLIDVNQEKAE
                     GEALDLLDGMAWGDENVAVWSGGYEECKDADIVVITAGINQKPGQSRLDLVKTNASIM
                     RQIVKEIMGSGFDGIIVVASNPVDILTYIAWNESGLPTSRVIGTGTTLDTTRFRKEIA
                     LKLKVDPRSVHGYILGEHGDSEVAAWSHTTVGGKPVFEIVEKDHRIAQDELDVIADKV
                     RNAAYEIIDRKKATYYGIGMSTARIVKAILNNEQAVLPVSAYLTGEYDEKDIFTGVPS
                     IVDENGVREVVELSINEEEKAMFSKSTSALREVLNTVL"
     gene            complement(3078..4022)
                     /locus_tag="IWO89_000880"
     CDS             complement(3078..4022)
                     /locus_tag="IWO89_000880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAQ0455653.1"
                     /translation="MRNEESIETLQREQGMNINDLNLAYHLQTPPKIHRGDLFQQGNI
                     HISKHRRYAEVPEHTHDFVEINYMYSGTCLQIINGERIEMEESDVVIIDKEATQRIEY
                     TGQEDILINILLKEETLSTEILNHLASTNNLLSSFLINASKEKGEHTQFIHFRGGKTS
                     RLTHLIENMLLIYFDTYSHKIRSLNLYTSLLLIELTHLLEQQSFSSADGCAENEMLRV
                     LDYIDKHFTSCTLKETAAHFGYNPVYLSNKLKMYFDASFQELVLKKKFDFALELIQET
                     SFSLEEIANRIGYKQTTSLFKLFKKYSTCTPNEWRNKH"
     gene            4249..5442
                     /locus_tag="IWO89_000881"
     CDS             4249..5442
                     /locus_tag="IWO89_000881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAQ0455654.1"
                     /translation="MNKKMMKFSLLSVSLILTSAGAVTSAIPSMSATYATRSLSAVEL
                     LTTAPSFMVTIFVLISSFIARKIGQKQTVIIGLVIASIYGILPLFSQSYSIVLASRAG
                     LGIGFGLINSLAVSMIAAFYHGDERASMIGFQSAFQGFGAALMTFVAGQLVQFGWQYT
                     FLVYAISLPILVLFVLFVPTPETTELQSHGTAPTRQVIKTNPKMISYGIFLLLVIVVY
                     NAVSIKLATVLTTYSLGNETNAANILTIMQLASMAAGVCFGWLQTKAHRYMLTLSLLL
                     MGIGFVLIATVINLYVIGFGAILTGISFSLFIPYLFNQVSIQAPTNTSILLVGANLGT
                     FIAPYGLLFLSFASLGSKTLAVFVNGSILLLISALISLLVVHRTNRKTVIRVDEEIKE
                     QLTTK"
     gene            5464..7479
                     /locus_tag="IWO89_000882"
     CDS             5464..7479
                     /locus_tag="IWO89_000882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-L-rhamnosidase"
                     /protein_id="HAQ0455655.1"
                     /translation="MTNEKHKDRWLWYPGDFEIRHGLLQNFQREERGFDWPAYWYMDD
                     CHRNVKFKRYYFLDQPSMFKVTIQGVGYVEINGQKHPCGKWLTCPAGKAKIRIFVGHT
                     SGLPAMFIEGDEVKSDIGWTASNFIEEYPAGWSPLYVDIAKDPNQIYYQKECVHPVTE
                     TEIGGGILFDFGRAVNGCVELISLDDHIESITLCYGESDVEAMDIDYCYYKQIGMTRK
                     MKARKRAFRYLFIPEVSTGMVQLQVFHEFLSLSKQAAFSSDSSLLNQIWSVSEETFKL
                     CSDLFFIDGIKRDRWIWSGDAYQSFMINQYLFFDEEINKRTILALRGQNEIKQHMNTI
                     VDYSILWLIGIENHYMMTKDYEFLRIVYPKMQSLMRYLMEQTNELGFIYGRAKDWIFI
                     DWSEMDKEGTLAAEQVLLLKAYHSMITCGDILAETYGENENYHTHLYQEKYQLLKQNL
                     FDYFWDEELGAFIDCYESGRRNVTRHANIFAVLFDFANKDQQQSILKNVLLNDTITQI
                     TTPYFKFYEQDALCKLGQTNIVYQTILDYWGGMLSHDAVTFWEEYDPTQTGNEKYSMY
                     GDPYGKSLCHAWGASPIYLLGRYFIGLYPTKPGYEEFMIQPNLDSFETLNCTLPIKDG
                     KISLEKTKDKIVITSDRTGGTVKMNGKSYPLLAKQPLVLTSYSKINA"
     gene            complement(7562..8935)
                     /locus_tag="IWO89_000883"
     CDS             complement(7562..8935)
                     /locus_tag="IWO89_000883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIC"
                     /protein_id="HAQ0455656.1"
                     /translation="MDKLLNLFVSIVSQPSILVSLIALLGLVLQKKKFSEVIQGTIKT
                     FVGFLVLIGGAGLISNSLTPFASMFQTALHTQGVVPSNEAVVAIALQQYGTPTALIML
                     VGMIVNILLARFTRYKFIFLTGQAMLYVSCLTAVILVSAGFTANLWMILLGGVFEGTL
                     LTITPALCQPFMKNITGNNQVALGHTGNIGYASSGWIGKFFGNKDRSTEDIKIPKSFS
                     FLRDSTVSIMLLMSIVYVALALLAGPGFVERELSNGDNAVIFALIQAGTFTAGFVIVL
                     QGVRMVLAEIVPAFQGIAKKLVPDSKPALDVPIVFPYAPNAVLIGFFVSFFVGTLSMF
                     AMIGLQTTVIIPGVVGHFFCGAASGVFGNSTGGRRGAVLGSAFNSLLISWLPLFILPV
                     LGDLKIASSTFADTDYLVPGLVLGKLGEYGQIALTVGIIAFFLLVVLSSFLFAGKRTN
                     IVSKEQN"
     gene            complement(8961..9245)
                     /locus_tag="IWO89_000884"
     CDS             complement(8961..9245)
                     /locus_tag="IWO89_000884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAQ0455657.1"
                     /translation="MKIFAVCQSGLGTSFMVEMNIKQALADLGADVDEFEVSHTDVGS
                     VSSDMADYFFIEHSLKDTLTSIPDEKLVPLQSIIDSEEVKEKVTKILSKE"
     gene            complement(9247..11262)
                     /locus_tag="IWO89_000885"
     CDS             complement(9247..11262)
                     /locus_tag="IWO89_000885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator"
                     /protein_id="HAQ0455658.1"
                     /translation="MNYKISYLLKTPREISLEKAAEIIEVNTSEAIKQLTPFAEYVDD
                     TRIRIKKFRAIDWAEKFLDITRFEAVYTEQERRDLLYLMVYSTATSVSVFHFQDFLQV
                     SKGTVLSDIKKIRNELEVEDCQLSYTRKTGFRLLGSEAILRREAKNRTARLMQSLAGR
                     FGLTYLTATLSIDYYAKVRDLLDKTISLSDLRFAPNRMDELAYYLSLTTIRIGKTSME
                     LVKDAELLASLAVTDISRQVLTGLGYSEHQNSEIIFFSLCLASIVEGDVQEPALDFLL
                     ECSSEVIHRMEFLMAVEFASFRELLMNVFYHLVPAYFRISYSFYLPNVMIDQIKHQYA
                     SIYEMTRKALLPLERRIGKSIPEEEIGFFTILFGGEIRKADAEERNRKIRAVIVCPSG
                     ISSSLILKSELQQLFPMIFFTETNSADRLHEVDETSYDIIFSTVPLTLTDKKKSLYVL
                     HPIMTDLEKNQLINQVQRDWLIPGFSMPSAQELLSALLPYIELKEGVDEEKLYQVLNR
                     KMNKLLEKKEDQRPMLSELLTEEMIQLSDQPKNWQEAITFAAQPLLSENKIEESYIKA
                     MIERVEQYGAFIHIGDHIALPHARPEDGVNEVGMSLLKLQQPVDLLDNPAHPISLFIC
                     LAAIDNEAHLRALANLTKLLSNKQNLQDLLHASTKEEILAIIRKGDE"
     gene            complement(11441..11746)
                     /locus_tag="IWO89_000886"
     CDS             complement(11441..11746)
                     /locus_tag="IWO89_000886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA processing protein"
                     /protein_id="HAQ0455659.1"
                     /translation="MTNYDGEKHKRLRGSMAPKTEEEVALAGTNSGETEDKEESLAGT
                     NSGETENKETALSGSNSVEEETKPNEPNHFEKDIEKAVEDVKEKFEELTSKNKHDHH"
     gene            complement(11835..12866)
                     /locus_tag="IWO89_000887"
     CDS             complement(11835..12866)
                     /locus_tag="IWO89_000887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dehydrogenase"
                     /protein_id="HAQ0455660.1"
                     /translation="MLTIAYLGNGKSTNRYHLPFSRKLTNKINIKKIFSPSEPVWKTF
                     EDIEYTDQLSDLWDDPEIQLVVITTPSQFHYEYGKMALEHGKNVLVEKPFAETSKEAE
                     ELFALAKEKGLFIQCYQNRRFDSDFLTVQKVIESGKLGDILEVEMHYDYFRPEVPENT
                     HEFSTANSYLYGHACHTVDQVLSYFGEPDDISYDVRQLLGKGRMNDYFDLDFFYGNMK
                     ISIKSSYFRIKERPSFVVYGKKGMFVKQTKDRQEEHLKMFYMPDHEDFGIDLPEHYGV
                     LTYIDEQGSYHEEKVISEVGDYSRVYEGIYETLVNGAEKVIKDEETILQMKILEEGIK
                     SIQESEGKI"
     gene            13073..13948
                     /locus_tag="IWO89_000888"
     CDS             13073..13948
                     /locus_tag="IWO89_000888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HAQ0455661.1"
                     /translation="MLIEEKLTKQELFTTTEKRIVDYIRRNIEAAVYMTIEELAKATY
                     TSHSAIIRLCKKMGFSGFKEFRFELGREVHQLIAQFNQTIDPNFPFSSEDSTQQIAKK
                     MADLSIQSIKKAQLQIEHEDLDQIGKVLNKAQRIFLFAKGDSQITARKFQNKMVKLNK
                     FLIMAEEYSDSSWNAANLTSEDCAIFISYSGRIHHYERIMTYLKHVEAPTLLITGNQH
                     SEMAKQADMCLVISQEEYDFAKVATFSSQIAFDYVLNTLFSVIYSQNFEENIRNLKNK
                     QELMQKGLLKEYRKT"
     gene            complement(14100..14942)
                     /locus_tag="IWO89_000889"
     CDS             complement(14100..14942)
                     /locus_tag="IWO89_000889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HAQ0455662.1"
                     /translation="MKPKKRKLLDLLMLFLLLSGIGILAYPFVSDALNNYLDQQIISH
                     YQQQAVKENEEVMAKIQENMTKKNQQLAKEGGNPGADPFTKKKEPAKKDRTYFEKHTI
                     GILTIPEINVHLPIFDETNTVLLEKGASLLEGTSYPTGGENTHAVISSHRGLPQAKLF
                     TDLPKLTLGDSFYIEINGRTLAYQVDQIKTVEPTDTKDLRIEKGQDFVTLLTCTPYMV
                     NSHRLLVRGHRVPYHAKEVQKEVQKVSENKRFFLYGLLAACILIFLLLIYLFRQSFKI
                     RNRS"
     gene            complement(15034..16911)
                     /locus_tag="IWO89_000890"
     CDS             complement(15034..16911)
                     /locus_tag="IWO89_000890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaH/EbpB family LPXTG-anchored major pilin"
                     /protein_id="HAQ0455663.1"
                     /translation="MKNHKKINVMLGVLFLILPLLTNSFGAKKVFAEETAAQVILHKK
                     KMTDLPDPLIQNSGKEMSEFDQYQGLADISFSVYNVTQEFYAQRDKGASVDAAKQAVQ
                     SLTPGTPVASGTTDADGNVTLSLPKKQNGKDAVYTIKEEPKDGVSAAANMVLAFPVYE
                     MIKQADGSYKYGTEELDTIHLYPKNTVCNDGTLKVTKIGTAENEALNGAEFIISKEEG
                     TPSVKKYIQSVTDGLYTWTTDQTKAKHFITGHSYDIGNNDFAEASIEKGQLIVNHLEV
                     GKYNLEEVKAPDNAEMIEKQTITPFEILANSQTPVEKTIKNDTSKVDKTTPQLNGKDV
                     AIGEKIQYEISVNIPLGIADKEGTQNKYTTFKLIDTHDAALTFDNDSSGTYAYALYDG
                     NKEIDPVNYSVTEQTDGFTVSVDPNYIPSLTPGGTLKFVYYMHLNEKADPTKGFSNQA
                     NVDNGHTNDQTPPSVDVVTGGKRFVKVDGDVTSDQTLAGAEFVVRDQDSDTAKYLSID
                     PSTKAVSWVSAKESATVFTTTSNGLIDVTGLKYGTYYLEETKAPEKYVPLTNRVAFTI
                     DEQSYVTAGQLISPEKIPNKHKGTLPSTGGKGIYVYIGAGVVLLLIAGLYFARRKHSQ
                     I"
     gene            complement(16908..18329)
                     /locus_tag="IWO89_000891"
     CDS             complement(16908..18329)
                     /locus_tag="IWO89_000891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaH/EbpB family LPXTG-anchored major pilin"
                     /protein_id="HAQ0455664.1"
                     /translation="MKKLGWLSMCLFLLLFKPAFTQVATETETEMVQITLHKLLFPNG
                     QLPKNHPNDGQEKALLQTYRGLNGVTFQVYDVTDSFYHLREKGKTVEEAQAEIAKNGA
                     SSGMFTAEATTTTLNNEDGIASFSLAAKDQEKRDKAYLFIESKVPEVVKEKAENMVVV
                     LPVHGQNNQKLSTIHLYPKNEENDYPDPPFEKVLEEPRNDFTIGEKITYSLHTTIPVN
                     ILDYQKFELSDSADEALTFLPNSLTISSNGEKLTEGFVIHKKPHGFDVLFSIPSLEKY
                     AGKKLTISYQMQLSSTAQANKEINNNGTLDFGFGVSTKKVSVYTGSKQFVKIETNKPD
                     KRLAGAVFLIKNKAGNYLQQTANGYKWTKNESDALHLISDKNGAFSISGLKTGSYRLK
                     EIEAPSGYILSETEIPFTISTFLSEDKEADSILKVVNKKENSRPFLPKTNETKNTLLG
                     VVGMVFASFAIWLFIKKRTGVKK"
     gene            complement(18332..21721)
                     /locus_tag="IWO89_000892"
     CDS             complement(18332..21721)
                     /locus_tag="IWO89_000892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HAQ0455665.1"
                     /translation="MTTTGKKLKVIFMLIILSLSNFVPLSAIADTTDDPTVLETISAE
                     VISDQSGKKALNIKLNANNTSAEKIEKEIGLVENYLSDVERKEGDGYAYQVNSGKITL
                     EISSNTKQTIDLSFPIDPALYHSQANKLIVDNKEYDIIDETENKKDTDVSVPKPDEIE
                     EESSKENENSVSPFTLPTLSLPAVSVPSNQTIPTEYTTDDQGTYPKASWQPTGNTNVL
                     DHQGNKNGTNQWDGINSWNGDPNDRTHSYIEYGGTGNQADYAIRKYAKETSTPGLFDV
                     YLNARGNVQKDITPLDLVLVVDWSGSMNDNNRIGEVKIGVDRFVDTLADSGITDKINM
                     GYVGYSSEGYSYSNGAVQMGSFDSVKNQVKSITPSRTNGGTFTQKALRDAGSMLSVPN
                     GHKKVIVLLTDGVPTFSYKVQRVHAQSSSNYYGTQFSNTQDRPGNTSLISRIYDAPDQ
                     NNLSRRIDSTFIATIGEAMALKERGIEIHGLGIQLQSDPAAGLSKAEVESRMRQMVSS
                     DEKGDLYYESADHATDISEYLAKKAVQISATVSNGQINDPIAEPFIYQPGTLSVKSVG
                     TSPTTVTPSISIEGNTIKSNQIYLGKDQEIQIHYQVRIQTENEDFHPNFWYQMNGRTT
                     FQPNIDTNELAEFGIPSAKAPGVSLHIKKLWEEFDNNLADRPDQVTFEIQREHTTNAA
                     AWKNGYIRIIKPAKDTTNTWERADIDKLSANSGESYQEILSLPQYNNQGQAFSYQTIK
                     ELPVPGYDSQQIDAMTWKNTKQFTPLNLKITKNSSTGEKDLIGAVFKLTGDSIDTLLT
                     DHGDGTYSLPENVKLQKEMTYTLTETKAPEGHGLSKKTTWEIKIASDGTVTIDGKTVT
                     TSDDTIQLTIENPFVEVPVAVRKYAMQGTDKEINLKGAAFSLQKKEANGTYQPIDSQT
                     TNEKGLASFDSLTPGKYRVVETAGPAGYDTSPGNYEFQIDKYGKIIYTGKNTEMTNNV
                     WTLTHQNRLKAFDLTVHKKEDNGQTLKGAKFRLQGPEMDLESPKDGQETDTFLFENLK
                     PGTYTLTETFTPEGYQGLKEPVTIVIHEDGSIQVDGQDHESVLSPGAKNNQISLDITN
                     QAKVPLPETGGIGRLGIYLVGMIGCAFSIWYLFLKKERGGS"
     gene            21978..23369
                     /locus_tag="IWO89_000893"
     CDS             21978..23369
                     /locus_tag="IWO89_000893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
                     /protein_id="HAQ0455666.1"
                     /translation="MEKIIRILSISDKWMTKKELAKRIGSSESTFVRYIEEIKIRWGT
                     DVTIQSSQKLGYRLEHLNVPAYLNVLMEMSQSSTNSRLLNEIIVNPGKTIEYYCEKIS
                     ISRSSFTRKLKQCNQILESYLLKVIVDQGFQLTSLKSELQLRIFVTFFFLSYYGRRTL
                     PFPIEKKKIKEIMVKNNCQMNLFQEESPYEQSFFIMYFVVHLMRESQGTPFEWKAGDS
                     YVQLINIDKKDFLILKNLLGKLKYSSYIKVVNYFVNTIFSEFSEKEKNEISEQIRKNM
                     SYCSFSSADCKEKNEEYVVQLLTQIYFFSRIFPYDTTHLTIRATYFAQQMQHRQIRLY
                     AGFRAELQFYSTLLNQNLIYYLSSYIFWMLIGNPEIHHYTSDKKILIISDLSLRHSQY
                     IEEYISDILAVHKIHSETTAITEDQLSKYNLLEYDLIVTNQPILNAHVPHILIDDSVS
                     FANEEELIRLFEL"
     gene            complement(23442..24089)
                     /locus_tag="IWO89_000894"
     CDS             complement(23442..24089)
                     /locus_tag="IWO89_000894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HAQ0455667.1"
                     /translation="MTMKGYPRIIRDKGEMWMPKETFFNLSNAKKQRIDDLLLEIFCS
                     QHISQVKVSEIVDKMGMSRGAFYKYFQDLEDAYLYTIKSSSHIVHQDILKFISENKQH
                     FFLGIEKYLEWCCDLDHESIYWQRLYLLTQRNDQQTTKRRELSADSPMNEKWLELLRI
                     NHFSIQSSEEAVSFLYFSMTLVMNTLTDLIANNWTKEELLKDFRYKSRWIAEGIQ"
     gene            complement(24097..24783)
                     /locus_tag="IWO89_000895"
     CDS             complement(24097..24783)
                     /locus_tag="IWO89_000895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002366355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ0455668.1"
                     /translation="MKKILTMKKVTKTFGSGHTKVSALKAIDFYVKEGEFVSIIGPSG
                     SGKSTFLTISGGLQTPTSGEIVINGHSLTELPEKKRADLRFKEIGFILQSSNLIPFLT
                     VKEQFELVDQVTSKKEDSTKIDSLLQSLDIQSLKNSYPKDLSGGERQRVAIARALYNE
                     PSLILADEPTASLDTDHAYEVVKLLVKEAHEKQKATIMVTHDVRMIQWSDRVYRMEDG
                     YLTEEEKKKE"
     gene            complement(24789..25862)
                     /locus_tag="IWO89_000896"
     CDS             complement(24789..25862)
                     /locus_tag="IWO89_000896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HAQ0455669.1"
                     /translation="MFLAIKEIRYSKLRYGLIVGIMFLIAYVVFMLSGLANGLSEEFK
                     KAIDDWDAQEIVLSEDANQVFAASQLTRGDLKYIEGGEKAPIGLYSGAIKGKNKENVT
                     VFGTTKEAFLLPKLTKGHEFKTKNEIIISQNLADSGYKIGDTIKIGSYDDPLTIVGIF
                     PETYYTVSPVIYASLDTWTALKYGQQPFASEEEQPINGIVIKDSAKISKEKASKGLQL
                     LTIGTFIESIPGYSAQNMTLNAMIYFLFLVVAAVVGIFMYVMTLQKTAIFGVMKAQGI
                     KNFFIAKSLVAQSFIVGVVGVLLALLFAYLTSLILPSAMPFAVFWSQWLLYSGILIIV
                     AMLGGLFSIRTITKVDPITAIGG"
     gene            26084..26758
                     /locus_tag="IWO89_000897"
     CDS             26084..26758
                     /locus_tag="IWO89_000897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAQ0455670.1"
                     /translation="MIKLLVVEDDKVLSNNIKEILAAIGEITQVYDGSEGLFELESGV
                     YDLAILDLMMPEMNGYEVLAKIRKEKIQTPVLILTAKDSLEDKIEGFERGADDYLTKP
                     FYREELIMRVKALLKRSLGLFDENVLKYKDISCDLKSNEVLYKEQVLPIQGKEFELLV
                     YFLQNKGIILTKEQIFDRIWGFDSDTTITVIEVYMSNLRKHLRSTGLDKEIKTLRNVG
                     YILQRV"
     gene            26763..28103
                     /locus_tag="IWO89_000898"
     CDS             26763..28103
                     /locus_tag="IWO89_000898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HAQ0455671.1"
                     /translation="MNGDITRKQRSRFFATNVAAFAFIFFLLGFITLQILNQSAYRET
                     DLSLEKINFDSRTVQLEIQRYQQGDPFLNPTQTEQNRTNMEQPLMEPSNDRFNTQVIL
                     WSKNGEILNKAAIGGRFNQIKELKMDTKNLNTIQNIELNEDSGSSALSFRSITKKAET
                     NQDGVAYIQILENTNQIKNSLATFRTIMILCMIVFWILSIGISYYLSSLSMRPIILSW
                     RRQKEFVENASHELRTPLTIIQNSLEHLFTKPNHTIIEESESIAQALSETRRLTGLTS
                     DLLTIARNDSDQQLLSKQMINTQEYIEQLVKPFQEMAIIDGKEFILHNDATLEVPIDE
                     KKIHQVLVILLDNALKYTNPEDKIILDSSVNHKEWLIHVKNTGPSISEEDQARIFDRF
                     YREDQSRAKETGGYGLGLAIAKQVIEQHHGQISVSNYLPQGVDFKVQLPLKPKK"
     gene            complement(28252..29238)
                     /locus_tag="IWO89_000899"
     CDS             complement(28252..29238)
                     /locus_tag="IWO89_000899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2382 domain-containing protein"
                     /protein_id="HAQ0455672.1"
                     /translation="MEEKNLTVIGSYSTREEALSVIERLRNEGYERDDIVIYTTDEAA
                     SRLGFDGLSGIDVETDENRMDGEEDRSLWEKIKDTFSFDTYDSETATPENDPLYQYRG
                     DISDGKFVITVKGYRQPETTEDTMDTQDTVSPASTMGTGLETGTGTDPMDVGGTTGFQ
                     NTTDPLNMTPGTDPMDVPGETDTMGTSKTEDIDRKPNLDDDTIQLKEEKLDVNTHDVT
                     TGEVDIHKHVVNDTETVEVPVKREEIVIERKPVTDQSSQGTDEKLEDDTITIPIKEEQ
                     VDVSKHTVIREEVGIHKEEHEDVEKVTEDVSREELDIDTSGDVHIEDRNKKS"
     gene            29962..30993
                     /locus_tag="IWO89_000900"
     CDS             29962..30993
                     /locus_tag="IWO89_000900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HAQ0455673.1"
                     /translation="MDSKTRKQLQEKLKQRTHLLPSVFQQFLEENRSSLSLGSRYEYA
                     KDFHLFTDYLRKVFSEEVDDQLIIHLEKQDYQNFLAKIYRHTRSFQTTANQETEQLFE
                     NSYYGISRKQYSLNHFLRFLYQKGLIEEEISLLGTSLPETTKAAPRYIDAELFKDFEH
                     LFIPIEGFQTSREASFEEKNRPRNLAITAILLYCGLKIHEVVELKRKDVSLTKQILNL
                     NRKENRLNKVPIPKEAMPFLENYFKLTAQNTDENSFVFRSSHDQQISPRTIRQILSKI
                     TVYKNVSPELCRKTYRYYAELYLDDADAVNYLCGNRYLSSHSFQDIWEKMVDFSYHEL
                     AAFVRVSSI"
     gene            31102..32634
                     /locus_tag="IWO89_000901"
     CDS             31102..32634
                     /locus_tag="IWO89_000901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA synthetase"
                     /protein_id="HAQ0455674.1"
                     /translation="MMETITYSPLNLFTNFQEAAANFSETPIIFDEPLSAFPELGLET
                     TYKKCCESVLKRAYQLAHLGVKSGDKVIIYKSSAFDTYLLAVSVSYLGAVPVMTSYHL
                     PTTTMEVFIDRLEDPFILFDDETKKRVREISNGTKSKQIPILYLMEQPALSVSQSFLD
                     KNEISYMTHTSGTTGIPKLICHSAHSMGWRTKWQKTVFTKISEKKLIAFHISPVHSRF
                     NIGISSLMSMGFPMMPLANAQSSKVVHMLEAHRPIALETHPNNFVQWRFTAKEHPEAF
                     SGIRYYHSTFDAINNQTMKTFLRTSMAQDAVFLQVYGQSECGPMILKAHTLESLKTSD
                     ARDMGVGLEDMTSARITDSVGNVLPENTDGHIQLLSKGRALTYYKEDARFQENVYGDW
                     WDSGDYGFMDKHGHLFLKDRQVDLIETINSTLAIEDFLLDSLDFLAEVVIVRDKNHSP
                     QPIIALAPDKEMDWNRWWEQVHELPRLNVPIIRDFDAIPRTATMKVQRLQIEKELKSQ
                     KS"
     gene            32773..33585
                     /locus_tag="IWO89_000902"
     CDS             32773..33585
                     /locus_tag="IWO89_000902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylpyrimidine/phosphomethylpyrimidine
                     kinase"
                     /protein_id="HAQ0455675.1"
                     /translation="MKRILTIGGSDPFAGGGIQTDLKTFENFGLFGLSALTSIGALDI
                     NERFMLESISLELLKRQLVSIDKMTSLDGIKIGLLNSSEAIMVVRDFIKTKQNIPIIL
                     DPVLAFKETEAVINQLYISQLIEELFPLVTMITPNLAEAALLSGQALPSSLTEMIHLA
                     KELTASGTKNIVIKGGTRMAGDEAVDLLYSIDHYEVFSHKKIDTTTVNGAGCAFSSAI
                     TANLVLGADFTKAVAESKKFVAHCISNGVLMNDHTGSVWSGGIQKGGMRNEN"
     gene            33575..34060
                     /locus_tag="IWO89_000903"
     CDS             33575..34060
                     /locus_tag="IWO89_000903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HAQ0455676.1"
                     /translation="MKTKQLTIYAMLIALTVALSLTILIPVPATNGFVTLCEAGIYTA
                     AFLYGPTGGLVVGASSGLLIDLISGYPQWAVFSLVIHGLQGYVAGSFSKSSTRMWGLG
                     LLLGSLVMIIGYGIAGWILYDWPAGVASIPGNIFQNLIGMGLAFPITASLQRIQARKQ
                     Y"
     gene            34083..34628
                     /locus_tag="IWO89_000904"
     CDS             34083..34628
                     /locus_tag="IWO89_000904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01440 family protein"
                     /protein_id="HAQ0455677.1"
                     /translation="MKLSEKELKDQLTEIVNDILAEAHLKKGDLFVLGCSTSEVVGGH
                     IGKNSSAEVGQWIIRTLKELLDPKEIALAVQGCEHLNRALVVERTVAEAKNFEIVSVV
                     PALHAGGACSVAAFDQFNDPVEVEHVVGQAGIDIGDTSIGMHIKHVQVPVRPRLKTLG
                     QAHVTALRSRPKYIGGPRANY"
     gene            complement(34701..36344)
                     /locus_tag="IWO89_000905"
     CDS             complement(34701..36344)
                     /locus_tag="IWO89_000905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase"
                     /protein_id="HAQ0455678.1"
                     /translation="MIQEQKWWKEAVGYQIYPASFKDSNGDGQGDINGIREKLNYLKD
                     LGIGFIWINPIYQSPFVDNGYDVSDYQDIEERFGTMEDFDLLLKEAHELGIKVIMDLV
                     INHSSDQHQWFEESKKSKESPYRDYYIWVDGVDGKEPNNWTSIFGGSAWEYSHETGQY
                     YLHVFAKEQPDLNWESEKLKEELFNMIRWWLDKGIDGFRLDAISHVKKDEYSVKATEN
                     PFSPFQNVSGIEEHLTDLKHVFEEYDIMTVGEASGVTAEEGPQWVGKDGYFDMIFEFD
                     HIHIWQQEKEGQLDVLKLKHALSAWQTSLDGIGWNALYMENHDVPRAVSVFGDTRPDF
                     WAMSAKAIAMMYFFLQGTPFIYQGQEIGMTNMPFESIDQVDAVDSKRLYKRLLAEGKT
                     REEALDIIRETTRDNSRTPMQWTSEQYAGFSTHEPWLITNPNTKTINVEQQEYEPESV
                     LQFYKNMIRIRQTNKGLIYGSYKEYLHEHPQLYVYERYLEDEQYLIMVNLTESLADYE
                     LPKEADQSWTLLLSNSSSGEFEAKGILAPYEARLYKTNK"
     gene            complement(36473..38701)
                     /locus_tag="IWO89_000906"
     CDS             complement(36473..38701)
                     /locus_tag="IWO89_000906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA domain-containing protein"
                     /protein_id="HAQ0455679.1"
                     /translation="MENFSLTENRTFSRLEKQMIWKKPKSHRTSAEEWRIASEIKGNW
                     QDPEMKISNVLLEGDAGSGKTQLAKALSADLQLPYTKVTCFADMDKSDVFGALLPVVD
                     SNKEQDQELLEAIYQTDTLEAVLTLIQNHYGIDPFSAKEKLAELVQRIEADYPDAIHY
                     SYYPSEIVRAIEKGYLLEIQEPTVIRDASVLVALNSALEPNGMLNLPTGIIRRHPDCV
                     IVITTNRNYQGNRPLNESLRDRMQHAEKMDLPALEVMAERAMAKTMIQKQELLLKMAE
                     IIRLLDETAKANAIKGVAGMRSYFYWANTYKQGQDLLQSIYPKVLYKLSTDSDELAIL
                     EQALSDSGLLLELQDILRKEKWGHPQSEHTKERTISAEEANERNITENAEEILRLDEK
                     KSLSAEEPNEGEEKQEETIEKNEIPPQMQKERSSDIEGKNQKEQQTGDDSQEMSASDM
                     EAHDKEIKKQLNKEARQIMKETIHEKEGLIVHRPAFQGKTPEARELHMQVMPIVESLS
                     RQILELLDNEQTETYQKGKYEGQRFNASKVAYGDLRNFDKKNPPHEQPSLAVAVRIDE
                     SGSMIRDDRIEAAKKAAIAIAEFSKKVDIPLLIYGDTADRSSREKTSLFSYKEFEDGF
                     QWLNEKLVTMKPRQNNRDGAALRLAAEKLNRQSATTKLLLNISDGQPKALPDYTGKKA
                     KEDIQSVLKEYERQGILFVSAAIGQDKEEIKSIYGESRFVDITDLNEFPKQMIQLIAR
                     YL"
     gene            complement(38734..39213)
                     /locus_tag="IWO89_000907"
     CDS             complement(38734..39213)
                     /locus_tag="IWO89_000907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0455680.1"
                     /translation="MEIPTNLKHKPVIVVEDYDKVDGRNALNMDAKGLSLGLAQWNDR
                     GKVDISGKVWRHTGEKWSRQSEELPITRILDLAILAAQGSLYFQDAYRYEKFYDPENP
                     VVDIIGLQGNRMTVEVDTKNPKIDEDIILYYDTLQKDGELIGERFRILKRLLDELGY"
BASE COUNT        11596 a         7388 c         7156 g        13273 t
ORIGIN      
        1 gtgaatcaag caaaaacgtt ggggtaggct gattttatat agatagcaag gagttttttt
       61 atttgaaaaa agtgcattca tttttaaatt atcttatggg agcaatcacg attgtggctg
      121 tgtaccaagc atttctaaag tatcctgatt acgggacgaa gttgttgtca ttgacgcaca
      181 atcaaacagc atattcacca acgttgattt tatcgacttt gactgcagga gctaaaagcg
      241 ctacttattt ttttcataaa acgacaagca tgctaaagcg aaagaaacag cgaaaacaat
      301 tgttggtggt ggtagcggct atgatcatta tttattttct accagttatt acacaagtat
      361 ttcatcaata ttttggttaa gaatacgaga gtttgtgaca aaagtcatgt cacaaacttt
      421 tttctttgtc attttttcgt aagaaaaagc aaaataaaag tatatacatt gattaaaagt
      481 atatacttgg atataatgta cttactatag aatgagaatg gtatagaggg tggcgaaata
      541 tgaaaaaaat gaaaaagtat aaaattagaa aatctggcaa tagcgatgta acaactatcc
      601 caccagaagt aaaagaattc atgggtgtgc agacaggtga cgcaatcagt tatgtttttc
      661 aatctgatgg ttctgtacga atgatcaaag cacaagaaga acctgatatt gattcattag
      721 ttgattcgat catgaatcaa tatgaagacg cgttaaaaga tttggttgat ttatgaggta
      781 tctttcgact aaagaaatta tcaaaatcaa cgcgaaagtt attttacgtc actcgccagg
      841 cgaaatgatc ggtgtaaaag atgcaaatgc attagatatg gccgtcaaac agccttcaca
      901 agcagtattc aatcaagaac tctatcctga aatctatgaa aaagcggcga ttttagcaat
      961 taatttagct aaaaaacatc cttttcataa tggcaataaa cgaactgctc ttgttgccat
     1021 gttgttgttt cttcaattga ataattgtcc agttgctttt tctcgtcaag aagctgttga
     1081 ttttatcata atgattacca ctagttcatt ggatttcgat tcactaaagt ccaagataac
     1141 cagttattta cgacaaaccg caataaaatg aacgaattaa aaactttatt tctcaattag
     1201 atcagcatgt cattaaggaa tatgctgatt tttttatgtt taatcataca aaatagtttt
     1261 ttcataaaaa tatggaaaat atggattaat tttgaaataa ttgatttaaa aggaatacaa
     1321 aagagtgaat agtaggaaat agtggtttag aactagtggg ctgatttttt atatatttta
     1381 gtttacataa tatatattat acaaagtaga tttaaaaaat gataaacctc tctcgaacgg
     1441 ttttttatca aaaatcgtca tttttccatc aaaatctgct tatgcaaaat tataaaaaaa
     1501 ttttgcagtt tttcttactt tttttgtcgt ttaattgaat gataaaaacc agtttcttta
     1561 ttcagttaat caaatatgaa cgaattagat atgaatcatt gtatttgccg ttgcggaagg
     1621 attttttaat taaccacaga taaacattga taacgattcg cttattgtaa tcaacagatt
     1681 aaatttcata tgttcatatg tacggataca ttttcggacc gattaatttt aaccattctg
     1741 tttatttgct tacctattag atatcaataa aaaactgctt aacagctatt tcagctgtta
     1801 agcagtttta ggatttttag ttcatattca ttgtttctta taatacagtg ttcaatactt
     1861 ctcttaaagc acttgttgat ttgctgaaca ttgctttttc ttcttcgttg attgataatt
     1921 caacaacttc acgaacacca ttttcgtcaa cgattgatgg cacacctgta aagatatctt
     1981 tttcgtcgta ttcaccagtt aaataagcag atacaggcaa tacagcttgt tcgttattca
     2041 agatagcttt tacgatacgt gcagtactca taccaatacc atagtaagtt gcttttttac
     2101 gatcaatgat ctcataagca gcattacgga ctttatcagc aatcacatct aattcatctt
     2161 gggcaatacg atggtctttt tctacaattt caaacactgg tttaccaccg acagttgtat
     2221 gtgaccatgc agcaacttct gaatcaccat gttcacctaa gatatagccg tggacgctgc
     2281 gtgggtcaac ttttaatttc aatgcgattt ctttacggaa acgagttgta tccaatgttg
     2341 ttccagtacc gataacacgt gatgttggca gaccagattc gttccaagcg atatatgtca
     2401 aaatatctac tgggtttgaa gcaacaacga taataccgtc gaatcctgat cccatgattt
     2461 cttttacgat ttggcgcatg attgacgcat ttgttttaac taaatctaaa cgagattgac
     2521 caggtttttg gttgatacca gctgtgataa caacgatatc agcatcttta cattcttcat
     2581 agccacctga ccatacagcc acattttcat cgccccaagc cataccatct aaaaggtcca
     2641 atgcttcccc ttctgctttt tcttgattca catcgatcaa tactaattca ttagagattc
     2701 cttggttgat cattgcataa gcgatacttg ttccaacaaa acctgtaccg acgattacta
     2761 ctttacgact tgtttttttc atgaataaac agctcctttt cataactatt tgttactttc
     2821 atgaaaagta tagtcttgtt tattcatttt atcaacatgt taattataat agttgttatt
     2881 ataatttttg atggaaacga attcctgaat acgcataaca gataaatgac aaatgtttac
     2941 acctatatcc cctgataata caaccaaaag tttagaactg aaaataaatc caaaaaaatt
     3001 cgctgcaacg atataataag acagcaatcg cacctaaaac aattttcctt catatgcttt
     3061 agtgaaagat tttcttctta atgtttattt ctccattcat tgggagtaca tgttgaatac
     3121 ttcttgaata atttgaataa agaagtcgtt tgtttatatc cgatacgatt agcgatttct
     3181 tccaacgaaa aactcgtttc ttgaatcagt tccaacgcaa agtcgaattt tttctttaga
     3241 accagttctt gaaaggaagc atcaaaatac attttcaatt tattactaag atagaccgga
     3301 ttgtagccaa aatgagctgc tgtttctttt aacgtacaag aagtaaaatg cttatcgata
     3361 taatctaata ctctaagcat ctcattttct gcgcatccat ctgctgatga aaagctttgt
     3421 tgttctaata aatgagtcaa ttcgatcagt aatagtgatg tatagagatt taacgaacgg
     3481 atcttatgac tgtatgtatc aaaataaatc aacagcatat tttcaatcaa atgcgtcaaa
     3541 cgacttgttt ttccgccgcg aaaatgaatg aattgtgtat gttccccttt ttcttttgat
     3601 gcatttatca aaaaagaaga aagtagatta tttgtgcttg ccaaatgatt caatatctct
     3661 gtagataagg tttcttcttt taataagatg ttgattaaaa tatcctcttg ccctgtgtat
     3721 tcgattcttt gagtagcttc tttatcaatg atcaccacgt cactttcttc catctctatc
     3781 ctttctccat taataatttg taaacatgta ccgctataca tataattgat ttcaacaaaa
     3841 tcatgtgtat gctcgggaac ttccgcatat cgacggtgtt tactgatatg aatatttccc
     3901 tgttgaaata gatcccctcg gtggattttt ggcggtgtct gaagatggta agctaagttt
     3961 aaatcattga tattcattcc ttgttcacgc tgcagagttt cgattgattc ttcgttacgc
     4021 aaaaaaaatg tctaattcag ctagttccaa ttgactccct cctttttgta agcggtttct
     4081 ttcgcttatt agttcacgct gtactgctat tatatgcaaa gctatttttg gtttcataaa
     4141 gactttctat ggttgggaca tgaacttcct gtgattccgc tattgagaga ataaccactt
     4201 catactatat ttttgataag cgcttacaaa aatgaaagga acatgcttat gaataaaaag
     4261 atgatgaaat tttctctgct gtcagtctct cttattttga cttctgctgg tgcagtcacg
     4321 tctgcaattc ccagtatgtc agctacttat gccacgcgtt ctttatctgc tgttgaatta
     4381 ttaacgactg ctccctcttt tatggtcact atttttgttt tgattagtag ttttattgct
     4441 aggaaaatcg gacaaaaaca aactgtgatc atcggattag tgattgcatc gatttatggt
     4501 atcctccccc tcttctcaca aagttacagt attgtcttag cttcaagagc tggactaggt
     4561 attggctttg gattgatcaa ttctttggca gtcagcatga ttgctgcctt ttatcacgga
     4621 gatgaacgag cctctatgat tggttttcaa agtgcatttc aaggatttgg tgcagcttta
     4681 atgacatttg ttgcaggaca gctagtacag ttcggttggc aatatacctt tttagtctac
     4741 gccatcagct tacctattct tgtcttgttt gttttgtttg ttcctacacc tgaaacaact
     4801 gaacttcaat cacatggcac agccccaaca agacaagtaa tcaaaacgaa tcctaaaatg
     4861 attagttatg gtatttttct cttgctggtc atcgtggtat ataatgctgt atcgattaaa
     4921 ttagccacag tcctgacgac ttattctcta ggaaacgaaa ctaatgcagc gaatatctta
     4981 acaatcatgc aattggcaag tatggctgcc ggagtttgtt ttggctggct tcagacaaaa
     5041 gcacatcgct atatgcttac actttcttta ctactgatgg gaatcggctt tgtattgatt
     5101 gccactgtga taaacctgta tgtgattggg tttggtgcga tcctaacagg aatttctttt
     5161 tcgttattca ttccttactt attcaaccaa gtaagtatcc aagcgccaac caatacttca
     5221 atcttattgg ttggcgctaa tttaggtact ttcattgcac catatggttt gctctttctc
     5281 tcttttgctt ctcttgggag taaaacactt gctgttttcg tcaatggcag tatcttgctg
     5341 ttgattagcg cgcttattag ccttctagta gtacacagga cgaatagaaa gacagtgata
     5401 agagtagatg aagaaataaa agaacaattg acaacaaaat aaatttgatg gaggtcgtgt
     5461 aaaatgacaa atgaaaaaca taaagataga tggctttggt atcctggaga ctttgagatc
     5521 cgccatggac tgctacaaaa tttccaacgg gaagaacgag gttttgactg gccagcgtac
     5581 tggtacatgg atgattgcca tcggaacgtc aagtttaaga gatattactt tcttgatcaa
     5641 ccttccatgt ttaaagtgac gatccaagga gttgggtatg tagaaattaa tggacaaaaa
     5701 catccatgtg gaaaatggct gacttgtcca gctggtaaag caaaaattcg tatctttgta
     5761 ggacatacat cagggctacc agcgatgttt atcgagggag acgaagtaaa atcagatatt
     5821 ggctggacag caagtaattt tattgaagaa tatccagctg gctggagtcc gctttatgtc
     5881 gatatcgcaa aagatccaaa tcaaatctat tatcaaaaag aatgcgtcca tccagtcact
     5941 gaaacggaga ttggcggagg aatcttattc gattttgggc gagcagtgaa tggctgtgta
     6001 gaactaatat ctctggatga ccatatcgaa tctatcactc tatgttatgg agagtccgat
     6061 gtagaagcta tggatatcga ttattgctac tataagcaaa taggaatgac aagaaaaatg
     6121 aaagctcgta aacgcgcttt tcgctatttg tttataccag aggtttctac tggaatggtt
     6181 caacttcaag tttttcatga atttttatct ctttcgaaac aagctgcttt ttcaagcgat
     6241 tcttctttac tgaatcagat ctggtcagta tcagaagaaa ccttcaagtt atgtagtgat
     6301 ttgtttttta tcgacggtat caaacgagat cgctggattt ggtctggaga tgcgtatcaa
     6361 tcattcatga tcaatcaata tctttttttt gacgaggaaa tcaataaacg aacgatatta
     6421 gcattaagag gacaaaatga aatcaagcag catatgaata ctatcgttga ctattcgatc
     6481 ttgtggttga ttggtatcga aaaccactat atgatgacga aagactatga atttttacgc
     6541 atcgtctacc ctaagatgca atcattaatg agatacttga tggagcaaac gaatgaatta
     6601 gggtttattt atgggagagc aaaagattgg atatttattg attggtctga aatggataaa
     6661 gaaggaacgc ttgctgcaga acaagtctta ttactgaaag cctatcattc catgatcact
     6721 tgcggagata tcttagctga aacatatgga gagaacgaaa attatcatac tcatctctat
     6781 caagaaaaat atcaactatt gaaacaaaac ctatttgact acttttggga tgaagaactt
     6841 ggcgctttta tcgattgcta tgaatctggg agaagaaatg tgacgcgtca tgcgaatatt
     6901 ttcgctgttt tattcgattt tgctaacaaa gaccaacagc aatcgatctt aaagaatgtc
     6961 ttattgaatg acactatcac acagattact actccatatt tcaaattcta tgaacaagat
     7021 gctttatgca aactcggaca gacgaatatc gtctaccaaa cgattcttga ttattgggga
     7081 gggatgttat ctcatgatgc ggtaactttc tgggaagaat acgaccctac tcaaacagga
     7141 aatgaaaagt acagtatgta tggagatcct tatggaaaaa gcttatgcca tgcatgggga
     7201 gcaagcccaa tctatctttt aggacgctat tttatcggtt tgtatccaac aaagccaggt
     7261 tatgaagaat ttatgataca acctaatcta gattcatttg aaacactgaa ttgtacgcta
     7321 cctatcaaag atggaaagat ttcgcttgaa aaaacaaaag ataagatcgt gattacttct
     7381 gatagaacag gcggaacagt gaaaatgaat ggtaaaagtt atcctcttct agcaaaacag
     7441 ccacttgtct taacaagcta ttcaaaaata aatgcataaa acaaaaaact gcttgcgaca
     7501 tatcagaaga ctttaaatct tcttctgata ttgtccaagc aatttttatt ttgaagtttg
     7561 cttaattctg ttctttagaa acaatgtttg ttctttttcc ggcaaaaaga aagctagata
     7621 atacgacaag taaaaagaaa gcgatgattc caactgtcaa ggctatttga ccgtattctc
     7681 ctagtttccc aagtacaagt cctggtacta gataatcagt atcggcaaat gtagaagaag
     7741 cgattttaag atctcctaat actggaagga taaataatgg cagccaacta atcaataagc
     7801 tattgaaagc agaacctaat accgcccctc ttctaccgcc agtcgagttg ccgaagacac
     7861 cagaagcagc tccgcagaag aaatgtccta ctaccccagg aatgataact gtagtttgca
     7921 agcctatcat agcaaacatg gatagtgttc ctacaaagaa gctaacaaaa aagccaatta
     7981 atacagcatt aggtgcatat ggaaaaacga taggaacatc taatgctggt ttagaatcgg
     8041 gtacgagttt tttagcaatc ccttggaatg ctggaacgat ttctgccaac accattcgaa
     8101 ccccttgaag gacgataaca aacccagctg tgaatgttcc agcttggatc agtgcaaaga
     8161 ttacagcgtt gtctccattg cttagttctc tttcaacaaa tccaggacca gctaacaaag
     8221 caagtgcgac atatacgatc gacatcaaga gcatgatcga tacagtagaa tctcgcaaaa
     8281 agctgaaact tttaggaatc tttatgtcct ctgtagaacg gtctttgttt ccaaaaaact
     8341 ttccgatcca tccactagaa gcataaccga tatttccggt atgccctaac gcaacttgat
     8401 tgtttccagt gatgttcttc ataaacggtt gacaaagagc aggcgtaata gttaatagtg
     8461 ttccttcgaa gaccccgcct aataagatca tccacaaatt tgcagtaaac cccgcgctta
     8521 ctaaaatgac tgctgttaga caagaaacat aaagcattgc ttgacctgta agaaaaatga
     8581 atttatatct tgtgaatcta gcaagtaaaa tattcacgat catccctact agcatgatca
     8641 aagcagtagg tgtaccgtat tgttgcaaag caatcgccac tactgcttca ttgctaggaa
     8701 ctactccttg agtatgcaga gctgtttgga acatcgaagc aaaaggggtt agtgaattac
     8761 tgattaaacc agcaccgccg atcagtacta ggaatccgac aaatgttttg atagttcctt
     8821 ggatcacttc tgaaaatttc ttcttctgaa gaacgagacc aagcagtgca atcaaagaaa
     8881 cgaggatgga gggctgactg acgattgaca caaacaaatt caacaattta tccattgata
     8941 gttcctccct tgaatcattt ttattctttt gacaagatct tcgttacttt ttcttttact
     9001 tcttcgctgt caatgatcga ttgcaaaggt acgagctttt catcaggtat agaagttagc
     9061 gtatctttca aagaatgctc aatgaagaaa taatcagcca tatcgctaga tacacttcca
     9121 acatccgtat gacttacttc aaactcgtcc acatctgctc caagatctgc aagtgcttgt
     9181 ttgatgttca tctctaccat aaaacttgtt cctaatccac tttgacatac tgcaaaaatt
     9241 ttcatatcat tcgtctcctt ttcttatgat cgctaatatt tcttcttttg ttgatgcgtg
     9301 taataaatct tgaagatttt gtttattaga aagtagcttt gttaaatttg ccaaagcccg
     9361 aagatgtgcc tcattatcga tagcagctaa acagatgaac aaactaatgg gatgggctgg
     9421 attatcaagc agatccacag gttgttgaag tttcagcaaa gacatcccta cttcgtttac
     9481 gccatcttct ggacgagcat gaggtaatgc gatgtgatcg ccaatatgga taaacgcacc
     9541 atactgctcc acgcgctcga tcatagcttt gatataactt tcttctattt tattttctga
     9601 taatagcggc tgtgcggcaa atgtgattgc ttcttgccag ttttttggtt gatccgataa
     9661 ttggatcatt tcttctgtta ataattcaga tagcatagga cgttgatcct ccttcttttc
     9721 taaaagtttg ttcattttac gatttaatac ttgatagagc ttttcttcgt ctacgccttc
     9781 ttttaattcg atataaggca gtagcgccga caacaattct tgggcagaag gcattgaaaa
     9841 tcctggtatc agccaatctc tttgtacttg attgatcagc tgattttttt ccaagtcagt
     9901 catgattgga tgcagaacat agagagactt tttcttatca gtaagtgtga gaggtacagt
     9961 agaaaaaata atatcgtaag aagtttcgtc aacctcgtgt aagcggtcag ctgaattagt
    10021 ctctgtaaaa aagatcatcg ggaataattg ctgaagttca ctttttaaga tcaatgaaga
    10081 gctaatgcca cttggacaga cgatcacggc tcttattttt ctatttcttt cttctgcgtc
    10141 cgcttttctg atctctccgc caaatagtat agtgaaaaaa ccaatctctt cttcagggat
    10201 gctcttccct attcgtcttt ctaacggaag taatgctttt cttgtcattt cgtagatgga
    10261 agcatattga tgcttaattt gatcgatcat cacattaggt aaataaaaag aataggaaat
    10321 acgaaaatag gcagggacca agtgataaaa gacattcatc aataattcac gaaacgaagc
    10381 aaattctacg gccatcagga attccatgcg atggatgact tctgaagaac attccagcaa
    10441 aaaatcaagt gcaggctcct ggacatctcc ttcaacgatc gatgctaagc aaagagagaa
    10501 aaatataatc tccgaatttt gatgttcgga atagccaagg ccagtcaata cttgtcgact
    10561 aatgtcagtg actgcaagag aagccaacag ttcagcatct tttaccagtt ccatggatgt
    10621 ttttccaatt cgaatggttg tcaaactcaa ataataagct aattcatcca ttcgatttgg
    10681 tgcgaatcga agatctgata gtgaaatcgt tttatctagc aaatctctaa ctttggcgta
    10741 ataatcaatg gaaagggttg cggttaaata cgtgagccca aatcttccag caagtgactg
    10801 catcaaacga gcagtgcgat tttttgcttc tcttcttaat attgcttctg accctagcag
    10861 cctaaaacca gttttcctag tatacgacag ctgacaatct tccacttcta attcatttct
    10921 tatctttttg atatctgaca ggactgtccc tttagaaacc tgaagaaaat cttgaaaatg
    10981 aaaaacagaa acagacgttg cagtcgaata aaccatcaaa taaagaagat ctcttcgctc
    11041 ttgttcggta tacacagctt caaatcgagt aatatccaaa aatttttctg cccagtcgat
    11101 tgctcggaat ttctttatac ggatacgggt atcatccaca tattcggcaa aaggagttaa
    11161 ttgtttgatc gcctcgcttg tatttacttc gatgatctct gctgcttttt ctagactgat
    11221 ttctcttggt gttttcagga gataactaat tttgtagttc acagcgctgt tcacctccta
    11281 cttatattct agtatggtag cgcttttaaa tcgaggggtg tttggtacaa cttacagttg
    11341 gatttttttt acgactgtac cgaacgattt ctatcgaatg gatacagaaa aaaagcaggt
    11401 gaccaaatgt ctcctgcttt tatgaattga tttcgtccga tcaatgatga tcatgtttat
    11461 tttttgaggt cagttcttca aatttttctt tgacatcttc aaccgctttt tcgatatctt
    11521 tttcaaagtg attgggctca ttaggctttg tttcctcttc tacagaattt gaaccagata
    11581 aagctgtttc tttattttct gtttcaccag agtttgtgcc agctaatgat tcttctttgt
    11641 cttctgtttc accagaatta gttcctgcta aagccacttc ttcttctgtt ttaggggcca
    11701 ttgaacctct taatcgtttg tgtttttcac cgtcataatt tgtcatgttg gttcctccta
    11761 gtagttctat actttttcat cgtacaataa gacagtttat gcgtcaaaaa acaaacccga
    11821 ttcttacaaa tatgtcatat ttttccttct gattcttgaa tgcttttgat tccttcttcc
    11881 aaaattttca tttgtaagat tgtttcttca tctttgatga ctttttctgc gccattaact
    11941 aatgtctcgt aaatcccttc gtagacacgg ctgtaatcac caacctcgga aattactttt
    12001 tcttcatgat aactcccttg ttcatctata taggtcaata caccataatg ttcgggcaaa
    12061 tcgataccaa agtcttcatg atcaggcatg taaaacattt tcagatgttc ttcttgtcga
    12121 tcttttgttt gtttcacgaa catccctttt tttccgtaaa cgacgaaact tggacgttct
    12181 ttgatgcgga aataactgga ttttatcgaa attttcatat ttccatagaa aaaatccaga
    12241 tcgaagtaat cattcattcg tcctttccct aaaagttgac gtacatcata gctgatatca
    12301 tctggttcgc cgaaataaga aagaacttga tctactgtat gacaagcatg tccatataaa
    12361 tagctgttcg cagtagaaaa ttcatgcgta ttttctggta cttccggtct gaaataatca
    12421 taatgcattt ctacttctaa aatatcccct aattttccgc tttcgattac tttttggact
    12481 gtcaagaagt cagaatcgaa ccgtctgttt tggtaacatt ggataaatag acctttttct
    12541 tttgctaatg caaataactc ttctgcttcc tttgacgttt ctgcaaaagg tttctctact
    12601 agtacattct taccatgttc gagtgccatt tttccatatt cataatgaaa ttgactaggt
    12661 gtagtgatga caactaattg gatctcagga tcatcccaaa gatcagacag ctgatcagta
    12721 tattcgatgt cttcgaatgt tttccacact ggttcagatg gggaaaaaat ttttttgata
    12781 ttgattttgt tcgtaagttt tcttgaaaaa ggcaaatgat agcgatttgt actctttcca
    12841 ttgccaagat aagcgattgt aagcatgagt ggtgcctcct tttattgatg ttttcagtat
    12901 atcctagaaa agcgcagaaa gtatttataa tagctattct ggtaaaaaag ttcagaaaaa
    12961 cacttgttat cattcggttt ggtgaaatta atgttcaaag taaggataaa aatcaaaaag
    13021 atagtagcgg aaccaagtgt ttatcagtaa gatagaattt ggaggtcaga tcatgcttat
    13081 tgaagaaaaa ctaacaaaac aagagttgtt taccacgaca gagaaacgga ttgtcgatta
    13141 catccgtagg aatatcgaag ctgcagtcta catgacgatt gaagaattag caaaagctac
    13201 gtatacttcc cattcagcta tcattcgttt atgcaaaaaa atgggattca gcggatttaa
    13261 agaattccgt ttcgagctgg ggcgggaagt tcatcaattg attgctcagt tcaatcaaac
    13321 gattgatcct aattttcctt tttcaagtga agatagcact cagcaaattg caaagaaaat
    13381 ggcggattta tcgatccagt caataaaaaa agcacaattg cagattgaac atgaagattt
    13441 agatcaaatc ggtaaagtat tgaacaaggc acagcgaatc tttctttttg caaaaggtga
    13501 ttctcaaatc acggcaagga aattccagaa taaaatggtc aagttgaata agtttttgat
    13561 catggctgaa gaatacagtg attcttcttg gaatgcagcc aatttgacta gcgaggattg
    13621 cgccattttt attagctaca gcggtcgcat ccatcattat gaacgaataa tgacctattt
    13681 gaagcatgta gaggccccta ccttgctgat taccggaaat cagcattcag aaatggccaa
    13741 acaggctgat atgtgtcttg ttatatcgca ggaggaatat gattttgcta aagtagcgac
    13801 tttctcctct caaattgcct tcgattacgt attaaatacg ttattctcag tgatatattc
    13861 tcaaaatttt gaagagaata tccgcaactt gaaaaacaaa caagaattga tgcaaaaagg
    13921 ccttcttaaa gaatatcgga aaacttaaga aaagatcctc atttagtaat aagaaagata
    13981 gtgtttgata ggctctcttt gatatcatca taaaaacagg ctgaagtttt ctttttcaaa
    14041 cctcagcctg tttgtttacg gaacgattcg aatagatata ttatagaaat gaggaatagc
    14101 taacttctgt ttcttatttt gaacgattga cgaaaaagat atataagaag gaggaaaatc
    14161 agtatacatg cagctaacaa tccatacagg aaaaatcgtt tgttctcgga tactttctgt
    14221 acttcttttt gaacttcttt tgcatgataa ggaacccggt gccctctgac aagtaagcga
    14281 tgactgttga ccatataagg tgtacaagtt aggagtgtca cgaaatcctg ccctttttcg
    14341 attctcagat cttttgtatc agtcggttct accgtcttga tctgatcgac ttgataagcg
    14401 agtgtccgtc cattgatttc gatatagaaa gaatcaccta aagtcagttt cggtaagtct
    14461 gtgaacaact ttgcttgagg cagtcctcga tggctggaaa taacggcatg tgtattttct
    14521 ccccccgtag gataagacgt tccttccaat aatgaggcac ctttttctag cagcacagta
    14581 ttcgtttcat caaaaattgg caaatggaca ttgatctcag ggatcgtcaa aataccgatc
    14641 gtatgtttct caaaataggt tctatccttt ttcgcaggtt cttttttctt agtaaaggga
    14701 tctgcacctg gatttccacc ttcttttgct aattgctggt ttttctttgt catgttttct
    14761 tggattttag ccatgacttc ttcattttct ttgaccgctt gttgctgata atgagagatg
    14821 atctgctgat ccagataatt attcagcgcg tcgctaacaa atggataagc tagtatccct
    14881 attcctgaga gaagcaagaa aagcatcagc agatctagaa gtttcctttt ctttggtttc
    14941 atttgtgttc tccttgtgat gactgtttga taataaaaga aaaaggagca tttgcgtaat
    15001 ccgctgctcc tttttccaat tcttgcaaaa agactaaatc tgactgtgct tgcgtctagc
    15061 aaagtacagt ccagcaatca gtagaaggac tactcctgca ccgatataca catagattcc
    15121 cttaccgcct gttgaaggaa gtgtaccttt gtgtttattt ggtatttttt caggagaaat
    15181 caactgtcct gctgttacat aagattgttc atcgatagta aatgctacac ggtttgttaa
    15241 tggaacatat ttttctggcg ctttcgtttc ttccagatag tacgtgccat attttagacc
    15301 tgtcacatcg attaaaccgt tacttgtggt tgtaaaaacc gttgctgatt ctttcgccga
    15361 tacccagctg acggcttttg tggatgggtc gatcgataaa tatttcgctg tgtcactatc
    15421 ttgatcacga acgacgaatt ctgctccagc aagtgtttgg tctgatgtaa cgtcaccatc
    15481 tactttaaca aatcgtttgc ccccagtaac gacatcgact gacggtggtg tttgatcatt
    15541 tgtatgcccg ttatcgacat ttgcttggtt agaaaatcct ttggttggat ctgctttttc
    15601 gttcaaatgc atatagtaaa cgaatttcaa tgtaccgcca ggagttaatg aaggaatata
    15661 attcggatca actgaaaccg tgaatccgtc tgtttgctca gtgacagaat aatttactgg
    15721 gtcgatttct ttatttccat catataaggc ataagcatac gttcctgaag aatcattatc
    15781 aaatgttaaa gcagcgtcat gagtatcgat aagtttgaat gttgtgtact tgttttgcgt
    15841 tccttcttta tcagcgatac ctaatgggat attgacagaa atctcatatt gaattttttc
    15901 accgattgcg acatcttttc cattcaattg aggtgttgtt ttatcaactt tagacgtatc
    15961 atttttgatg gtcttttcta ctggtgtttg gctatttgcc aggatctcaa aaggcgtgat
    16021 tgtttgcttt tcaatcattt ccgcattatc aggagctttt acttcttcta aattatattt
    16081 tccaacttct aaatgattaa cgatcaactg gcctttttca atagatgcct cggcaaagtc
    16141 attgttgccg atgtcataag aatgaccagt aatgaaatgt ttggctttgg tttgatcagt
    16201 tgtccaagtg tacaatccat ctgtgacact ttggatgtat tttttgacgc ttggtgttcc
    16261 ttcttcttta gaaataataa attctgctcc atttagtgct tcgttttcgg cagtaccgat
    16321 ttttgtaact ttcaacgttc catcattaca gactgtattt ttagggtaga gatggatagt
    16381 atctagttct tctgtcccgt atttataaga gccatctgct tgtttgatca tctcatatac
    16441 agggaaagct aaaaccatgt ttgcggcagc tgacactccg tcttttggtt cttctttgat
    16501 cgtgtagact gcatctttcc cattttgttt tttaggtaaa gataaagtga catttccatc
    16561 agcatctgtc gttcctgaag caactggtgt accaggagtc aaagactgga ctgcttgttt
    16621 tgctgcatcc acggacgctc ctttatctcg ttgcgcataa aattcttgag tgacgttata
    16681 aactgaaaat gaaatatcgg ctaatccttg gtattgatcg aattcgctca tttctttccc
    16741 gctgttttgg attaaaggat cgggtaaatc agtcattttc tttttatgaa ggatgacttg
    16801 agctgctgtc tcctctgcaa acactttttt tgcgccgaag ctgtttgtga gtaatggtaa
    16861 aataaggaaa aggactccta acataacgtt tattttttta tgatttttca ttttttcact
    16921 cctgttcttt ttttgataaa caaccagatt gcaaagcttg cgaataccat accaacaacg
    16981 cctaaaagtg tatttttcgt ttcgtttgtt tttggaagaa atggacggct attttctttt
    17041 ttattgacta ctttcaatat actgtccgcc tctttatcct cagaaagaaa agttgaaatg
    17101 gtaaacggaa tttctgtttc acttaaaata taaccagaag gtgcttcgat ctcttttaat
    17161 cgataacttc ctgttttcaa cccggaaatt gaaaaagcgc catttttatc ggaaatcagg
    17221 tgaagcgcat ctgattcgtt ctttgtccac ttgtatccgt tggctgtttg ctggaggtaa
    17281 tttcctgctt tgtttttaat aaggaatact gcgccagcta atcgtttatc tggtttattt
    17341 gtctcgattt tgacaaattg cttactccct gtatatacag agactttctt tgtactgaca
    17401 ccaaaaccaa aatccagtgt tccgttgttg ttgatttcct tgttcgcctg tgctgtactg
    17461 cttagctgca tctgataaga aatggtcagt ttttttccag catatttttc caacgaaggg
    17521 atcgaaaata aaacatcaaa tccgtgaggt ttcttgtgta tgacaaagcc ttctgtcagc
    17581 ttttctccat tcgatgaaat cgttaaacta ttaggtaaaa acgttaatgc ttcatccgca
    17641 ctatctgaca attcgaactt ttgatagtca aggatattta caggaattgt cgtatgcaag
    17701 gaataagtga ttttttcacc aatcgtaaaa tcatttctag gctcttctaa taccttctca
    17761 aaaggtggat cagggtagtc gttttcttca tttttaggat acaaatggat agttgaaagt
    17821 ttttgattgt tttgtccatg tacaggaaga acaactacca tattctctgc cttttctttg
    17881 acgacttctg gtactttgga ttcaatgaaa agatacgctt tatctctttt ttcttgatct
    17941 ttagcggcca gagaaaaaga agcgatacca tcttcgttgt taagagttgt agttgttgct
    18001 tctgcggtaa acataccgga agacgcaccg ttttttgcga tctctgcttg tgcttcttct
    18061 accgttttgc ccttttcccg tagatggtaa aaagaatctg tgacatcata aacttggaat
    18121 gtgacaccat ttaatcctcg atacgtttgt aataaagctt tttcttgtcc gtcatttgga
    18181 tgatttttcg gcagttgccc gtttgggaaa agcaatttgt gtaaagtaat ctgaaccatt
    18241 tctgtttctg tttctgttgc tacctgagta aaagctggtt taaatagtaa caagaagaga
    18301 cacatactaa gccaaccaag ttttttcata tttagctgcc ccctctttct tttttcaaaa
    18361 aaagatacca aatagaaaac gcacaaccaa tcatccctac tagatagatt cctaaacggc
    18421 caattcctcc cgtttcaggt aatggtactt ttgcctgatt cgtgatgtct aaagaaatct
    18481 ggttgttttt ggctcctggt gacagaacag attcatgatc ttgtccatcc acttgaattg
    18541 acccatcttc gtgtataact atagtaactg gctcttttag accttggtat ccttctggtg
    18601 taaaagtttc ggtcagcgta taagttccag gttttaaatt ttcgaataga aaggtatctg
    18661 tttcttgtcc atcttttggc gattctaagt ccatttctgg tccctgcagt ctgaattttg
    18721 ctccttttaa tgtctgtccg ttgtcttctt ttttgtgtac cgttagatca aacgctttta
    18781 gtcgattttg atgagtgagc gtccatacat tatttgtcat ctcggtattt tttcccgtgt
    18841 aaatgatttt tccatattta tcgatttgga attcataatt tcccggcgaa gtatcatatc
    18901 cggcaggacc agctgtttca acgactcgat atttaccagg tgtgagtgaa tcaaaactgg
    18961 caagaccttt ttcattcgtt gtttggctgt caattggctg ataagtacca tttgcttctt
    19021 ttttctgtag ggaaaatgct gctcctttaa gatttatctc tttgtccgtc ccttgcatcg
    19081 catacttacg tactgctaca ggaacttcaa caaaaggatt ttcaatagtc aactggatcg
    19141 tatcatcgga agtagtgact gtttttccat caatggttac cgtaccatca gaagcgatct
    19201 tgatttccca agtagtcttt ttgcttaatc catgcccttc tggagctttt gtttctgtca
    19261 gcgtataggt catttctttt tgcaatttga cattttctgg aagagaatag gttccgtcgc
    19321 catgatctgt tagtaaagta tcaatagaat ctcctgttaa tttgaaaaca gcgccaataa
    19381 gatccttttc acctgtagag gaatttttcg ttattttcaa gtttaacggt gtgaattgtt
    19441 tagtattttt ccatgtcatt gcatctattt gttgagaatc gtatcctggt acaggtaatt
    19501 ctttgattgt ttggtaactg aatgcttgac cttgattatt gtattgaggt agtgataata
    19561 tctcttgata actttctccg ctatttgcag ataatttgtc aatgtctgca cgttcccacg
    19621 tatttgttgt atctttagct ggtttaatga ttcgaatata tccgtttttc caagctgcag
    19681 catttgtcgt atgttcccgt tgaatctcaa aagtaacttg atctggacga tcagctagat
    19741 tgttgtcaaa ttcttcccat aactttttga tgtgaagact gactccggga gctttagcag
    19801 atggtatacc gaattcagct aattcattgg tatcaatgtt tggctggaaa gttgtcctgc
    19861 cgttcatttg ataccagaaa tttggatgga agtcctcatt ttctgtttgg attctcactt
    19921 ggtaatggat ttggatttct tggtcttttc ctaaatagat ctgattgctc ttgatggtat
    19981 ttccttctat ggaaatagat ggagtgaccg ttgtaggact tgtccccaca ctcttgactg
    20041 aaagtgtacc aggctgataa atgaatggtt ctgcgattgg atcatttatt tgtccattgc
    20101 ttacagttgc tgagatctgt acagcttttt tggctaggta ttcagagata tctgttgcat
    20161 gatcagctga ttcatagtaa agatcgcctt tttcatctga tgaaaccatt tgtcgcatac
    20221 gagactctac ttctgctttt gagagaccag ctgccggatc gctttgaagt tggatgccaa
    20281 gaccatgtat ttcgattcct cgttctttga gtgccatcgc ttctccgatg gttgcgataa
    20341 acgtactgtc gattcttctg gatagattgt tttggtcagg tgcatcatag attcttgaga
    20401 ttagagaagt atttcccggc cgatcttgcg tattagaaaa ctgagttccg taataattgc
    20461 tgcttgattg tgcgtgtacc cgctgtactt tataggaaaa tgttggtaca ccatccgtca
    20521 gcaaaacgat cactttttta tgtccatttg gaacggatag catgcttcct gcatctctta
    20581 gtgctttttg tgtaaaagta ccaccatttg tccgtgaagg tgtaatggat tttacttgat
    20641 ttttcactga atcaaatgaa cccatctgta ctgcaccgtt actgtagcta tatccttcgc
    20701 ttgagtagcc gacatatccc atattgattt tgtctgtgat accgctatct gctaaagtat
    20761 cgacaaaacg atcgacacca atctttactt caccgatccg attattgtcg ttcatacttc
    20821 ctgaccagtc tacgaccaat acgagatcaa gaggcgtgat atctttttgt acatttccac
    20881 gagcattcaa ataaacatca aacaatccgg gtgtacttgt ttcctttgca tactttcgta
    20941 tcgcatagtc tgcttgatta ccggttcctc catattcgat atacgaatgg gtccgatcat
    21001 taggatctcc attccaagaa tttataccat cccattgatt tgttccgttt ttattgcctt
    21061 gatgatcaag aacatttgta tttcctgtag gttgccaact ggctttagga taagtgccct
    21121 gatcatctgt tgtatattct gtaggaatcg tttgattaga tggcacactc acagctggca
    21181 aggataatgt aggcaatgta aatgggctga cagaattttc gttttctttt gatgattctt
    21241 cttctatttc gtctggcttt ggtactgaca catctgtatc tttcttattt tctgtctcat
    21301 caataatgtc atattcttta ttatcgacga tcagcttgtt tgcctggctg tggtaaagtg
    21361 ctggatcgat tggaaaactc agatcgatag tttgtttagt gtttgatgag atttccaacg
    21421 taattttccc gctatttacc tgataagcat agccatctcc ttcttttctt tccacatcac
    21481 ttaagtaatt ttcgactaga ccaatttctt tttctatctt ttcagcactg gtgttattcg
    21541 catttagctt gatgttcagt gctttttttc cagactgatc cgaaatgact tcagctgaaa
    21601 ttgtttctaa aactgttgga tcatctgtag tgtctgctat tgcagataat ggcacaaagt
    21661 ttgataaact caatattatc agcatgaaaa taactttcag tttcttccct gtggtcgtca
    21721 tttttcccct cctttttctt tttttctgtt ttgacgtgaa tagtgaaaat tattaaaata
    21781 aacaggcgtt acataatttt atttatttta atatgataat tattatacga agggcgctaa
    21841 agggaatcac accaattttc ataccaattg gtttcattta tgaagttgat tccttcgatt
    21901 aatgagcgtt tcattgaaag aaaagggttg agggagaaaa acatatgatc aggttgttgg
    21961 aatgcaacta tagaaagatg gaaaaaatca ttcgaattct gtccatttct gacaagtgga
    22021 tgactaaaaa agaacttgcc aaacgaattg gtagttcaga atctacattt gtaaggtata
    22081 tcgaagagat caaaatccgc tggggaacgg atgtaacgat tcaatcttca caaaaattgg
    22141 ggtatcgtct tgaacatttg aatgtaccag cttatttaaa tgttctgatg gaaatgtcgc
    22201 aaagttctac aaacagtcgt ttattaaatg agattattgt caatccgggt aaaacgattg
    22261 agtattattg tgaaaaaatt tccatcagcc ggtcttcttt tacacgtaag ttgaaacaat
    22321 gcaatcaaat tttagaaagc tatttattaa aagtgatcgt tgaccaaggt tttcagttaa
    22381 ctagccttaa aagtgagctc cagttgcgta tctttgttac ctttttcttt ttaagttact
    22441 acggaagacg cacgttgcca tttccaatcg aaaaaaagaa gatcaaagaa attatggtca
    22501 agaataattg tcagatgaat ttgtttcagg aagaaagccc atatgagcaa tctttcttta
    22561 ttatgtattt tgttgtgcat ctcatgagag aatcccaagg aactccattt gaatggaaag
    22621 ctggagactc ttatgtacag ttgataaata tcgataaaaa agacttctta atcttgaaaa
    22681 atctcttagg aaaattgaag tacagcagct atataaaagt agtgaactat tttgtcaata
    22741 cgattttttc agaattttcg gagaaagaaa aaaatgaaat cagtgagcag atacgaaaaa
    22801 atatgtccta ctgttcgttt tcatccgctg attgcaaaga aaaaaacgaa gaatatgttg
    22861 ttcaattatt aacgcaaatt tattttttct cccgtatttt cccatacgat acgactcatt
    22921 tgacgatccg tgcaacttac tttgcacagc agatgcaaca ccgccagata cgtctatatg
    22981 ctggatttcg tgcagaactt caattttatt ccactctact gaatcagaat ctgatttatt
    23041 atttatctag ctatatcttt tggatgctta tcggtaatcc tgaaattcac cattatactt
    23101 ctgataaaaa aatactgatt atcagcgact taagtcttcg acatagtcaa tatattgaag
    23161 aatatatcag tgatattctt gctgtgcata aaattcactc tgagacaact gctattactg
    23221 aagatcagct gtcgaaatac aatcttctcg agtatgatct gatcgttacg aatcaaccca
    23281 ttttgaatgc ccatgttcct catatattga ttgatgatag tgtgtcattc gcaaatgaag
    23341 aagaactcat ccgcttattc gagttatgaa taatcaaaaa acagctatgt ataaaccacc
    23401 ctgataaaaa tttcaggaca aatacacata gctgtttctt tttattgtat tccttcagca
    23461 atccaacgag atttatagcg aaagtctttt aacaactctt cttttgtcca attatttgca
    23521 atgagatccg tcaaagtgtt cattacgagg gtcatggaaa agtataggaa actaactgct
    23581 tcttccgacg attgaatgga gaaatgattg attcttaata attccagcca tttttcgttc
    23641 attggagaat cagccgatag ttctctacgt ttggttgtct gctgatcatt tcgctgggtt
    23701 aacaagtata acctttgcca atagatactt tcgtgatcta agtcgcagca ccactctaaa
    23761 tatttttcga tccccaaaaa gaaatgttgt ttgttttcgc taatgaattt taagatatct
    23821 tgatggacta tgtgagaact gcttttgatc gtatacaaat aagcatcttc caaatcttga
    23881 aaatatttat aaaaagcacc gcgcgacatc cccatcttgt caacgatttc tgagactttg
    23941 acttggctga tgtgctgtga gcaaaatatc tccagtaata ggtcatcgat tctctgtttc
    24001 tttgcgttcg ataaattgaa aaaagtttct tttggcatcc acatctctcc tttatctctg
    24061 attatacgtg gatagccctt cattgtcatg aaggattcat tccttctttt tctcttcttc
    24121 tgtcaagtac ccatcttcca ttcgataaac gcgatcactc cattgaatca tacggacatc
    24181 atgagtaacc ataatagtcg ctttttgttt ctcatgagct tctttaacta gtaatttgac
    24241 gacttcatat gcatgatctg tatccaaact agctgttggt tcgtctgcta agatcaagct
    24301 tggctcatta tataaagcgc gagcaattgc cactctctgt ctttctccac cagaaaggtc
    24361 tttaggataa ctatttttaa gtgactggat atctaaagat tgcagcaagg aatcgatttt
    24421 tgttgaatct tctttttttg aagtaacttg gtcaactagt tcaaattgtt cctttaccgt
    24481 taaaaaagga atcaaattag agctttggag aataaatcct atttctttaa agcgtaaatc
    24541 tgctcgtttt ttttctggaa gctcagttag tgagtgaccg ttgatcacta tttctccgga
    24601 agtcggtgtt tgcaatcctc ctgaaatggt aaggaaagta cttttacctg aaccagaagg
    24661 accaatgatg ctgacgaatt ctccttcctt tacataaaaa tcaattgctt tcaaagctga
    24721 gacctttgta tgaccagaac caaatgtttt ggtaactttt ttcatcgtta atattttttt
    24781 catttttatt aacctcctat tgctgtaatt gggtctactt tggttatcgt acgtatcgaa
    24841 aacaatccgc ctagcattgc gacaatgatc aaaatcccac tatatagcaa ccattgtgac
    24901 caaaaaacag caaaaggcat cgcgcttggt aaaatcagac tagtcaaata agcaaacaaa
    24961 agagcaagca gaacgcctac caccccaaca ataaaggact gtgctactaa agatttagcg
    25021 atgaagaaat ttttgatccc ttgtgctttc attactccaa aaatagcagt cttctgaagt
    25081 gtcatcacgt acatgaaaat accgaccacg gcagcaacca ctaagaacaa aaagtatatc
    25141 atcgcattta aggtcatatt ttgggcagaa tagccaggaa tactttcaat gaatgtccca
    25201 atagttaata actgtagacc cttcgatgct ttttctttcg aaattttagc tgaatccttt
    25261 attacgatcc cattgatcgg ctgttcttct tcagaagcaa acggctgttg tccatacttc
    25321 aaagcagtcc acgtatctag ggacgcatag atgacgggag aaactgtgta ataagtttca
    25381 ggaaagatac cgacaatcgt caaaggatcg tcgtaactgc caattttgat cgtatctcca
    25441 attttatagc ctgaatcagc cagattttga gagatgatga tttcattctt tgttttaaat
    25501 tcatgccctt tcgtgagttt tggtaataaa aatgcttctt tcgttgtgcc gaaaactgta
    25561 acattttctt tgttttttcc tttgattgct ccgctgtaca atccgattgg tgctttctct
    25621 cctccttcaa tgtatttcag atctcctctt gttaattgtg aagctgcaaa tacttgattt
    25681 gcatcctccg acaaaacaat ctcttgtgcg tcccaatcat cgattgcttt tttaaactct
    25741 tctgaaagtc cattcgctag tcctgacaac atgaatacga catacgcaat caagaacata
    25801 ataccgacga tcaagccata tcgtaattta gaatagcgaa tttctttgat agctagaaac
    25861 atttgttttt cctccctatg atagattgat agtgacaaaa tgtcacttgt tgaatagtgt
    25921 aactctaaag tgacaatttg tcacttgtgt tgtttttttc ttatcaataa attgtctaag
    25981 acatctttcg ataaaacctt aacatgagtc ataaaaaatt tatttaagcc ttatttatgc
    26041 tttccatgat aaactaaagt gaccaaatca atgaggtggg aaaatgatta aactattagt
    26101 agtagaagac gataaagtat tatccaataa tataaaagaa atactcgctg caatcggcga
    26161 gataacacaa gtttatgatg gtagtgaagg actgttcgag ttggaaagtg gagtttatga
    26221 tcttgcaatc ttggatttga tgatgccgga aatgaatgga tatgaagtgt tagcaaaaat
    26281 acggaaagaa aaaatacaga ctccagtatt gattttgact gcaaaagata gtttggaaga
    26341 taagattgaa ggatttgaac gaggtgcaga tgattatcta acaaaacctt tctatcgaga
    26401 agaactgatc atgcgcgtga aagcattatt gaaacgctcg ctaggtctct ttgatgaaaa
    26461 tgttctgaag tataaggata tcagttgtga tttaaaaagc aatgaagtct tgtataaaga
    26521 acaagttttg cccatacaag gaaaagagtt tgaattgctc gtttattttt tacaaaataa
    26581 gggaatcatt ttgacaaaag agcagatatt tgaccggatc tggggatttg attctgatac
    26641 gacgattaca gttattgaag tttatatgag caacttacga aaacatctgc gaagtactgg
    26701 attggataaa gaaatcaaaa ccttacgtaa tgtcggttat atactgcaaa gggtgtgatg
    26761 caatgaatgg agatattaca cgaaagcaac gtagtcgctt ttttgcgaca aatgtagcag
    26821 catttgcttt tatctttttt cttttgggat tcattacttt acagatatta aaccaatctg
    26881 catacagaga aacagatctg tcattagaga aaataaattt tgattctcga acagtacaat
    26941 tagagataca aagatatcag caaggcgatc cctttttaaa cccgactcaa acggaacaaa
    27001 acagaacaaa catggaacag ccattaatgg aaccatcaaa tgaccgtttt aacactcaag
    27061 tgatcttgtg gtctaaaaat ggtgagatat tgaataaagc agctattggt gggcgtttca
    27121 accaaatcaa agaattaaaa atggatacga aaaatctgaa taccatccaa aatattgaat
    27181 taaatgaaga cagtggctca tctgccctct ctttccgttc gattacgaaa aaagcggaga
    27241 cgaatcaaga tggcgttgcc tacatccaaa ttttagaaaa tacaaatcaa atcaagaatt
    27301 cattagcgac ttttcgcacg attatgattt tgtgtatgat tgttttttgg attctctcta
    27361 ttggtatcag ttattacttg tctagtctaa gcatgcgccc tattattctt tcttggagaa
    27421 gacaaaaaga atttgttgag aatgcttctc atgaattgcg cacaccgtta acgattattc
    27481 aaaatagttt ggaacatctt tttaccaagc ctaatcatac tattattgaa gaatccgaaa
    27541 gcatcgctca agcattgtca gagacgaggc gcctcactgg attgacttcg gatttgctga
    27601 caatcgctcg gaatgattct gatcagcagc tgctttcaaa gcaaatgatc aatacacaag
    27661 aatatatcga acaacttgtt aaaccatttc aagagatggc catcatagat ggaaaagagt
    27721 ttattctcca taacgatgca acattagaag taccaattga tgaaaaaaaa atccatcaag
    27781 ttttggttat tttgctggat aatgcattaa aatatacgaa tccagaagat aagatcattt
    27841 tggattcatc tgttaaccat aaagaatggc tgattcacgt aaagaatact ggcccttcta
    27901 tcagtgaaga agatcaagca aggatttttg atcgatttta tcgggaagat caatctcggg
    27961 cgaaagagac tggcggatac gggcttggct tggcaattgc aaaacaggta atcgaacagc
    28021 atcatggaca gatttctgta tccaattatc ttccacaagg agtcgatttt aaagtccaat
    28081 tgccgttgaa gcctaaaaaa taatgcttat ccctaaaaaa gggctgtgac aaaaatcttg
    28141 tcacagccct tttttgttgc gaataaacgg tgttccagaa gtagcttctt cggaaataag
    28201 ccgaaatttc tttaagctga ccacttctgt cacaacctct ttagcttacg cttacgattt
    28261 tttattgcgg tcttcaatgt gtacatcacc gcttgtgtca atatccaatt cttctcggct
    28321 aacatcttct gtcacttttt caacatcttc atgttcttct ttatgaatcc caacttcttc
    28381 acgaataact gtatgtttgc ttacatctac ttgctcttct ttgattggga tagtaatcgt
    28441 gtcatcttcc aatttttcgt ctgttccctg tgaagattga tctgttactg gtttgcgttc
    28501 gatgacgatt tcttcacgtt tgaccggtac ttctaccgtt tcagtatcgt tgaccacatg
    28561 tttgtggatg tcgacttctc cagttgtcac atcatgtgta tttacatcta atttttcttc
    28621 ttttagttga attgtatcat catctagatt tggtttgcga tcgatgtctt cagtcttgct
    28681 tgtacccatt gtatcagtct cgccaggtac gtccatcgga tcagttcctg gtgtcatatt
    28741 caatggatcc gttgtatttt gaaagcctgt cgtgccacct acgtccattg gatctgttcc
    28801 agtgccagtt tctaatccag tccccattgt gcttgctggg ctaacagtat cttgtgtatc
    28861 cattgtatct tctgttgttt ctggctgacg atagcctttt actgtgatga caaattttcc
    28921 atcagaaata tctcctctat attgatataa aggatcattt tctggtgtag ccgtctcaga
    28981 atcataggta tcaaaagaaa aagtatcttt tattttttcc cataaagaac ggtcttcttc
    29041 accatccatc cgattttcat ctgtttcaac atctatacca gacaatccgt caaatcctag
    29101 tctagatgct gcttcatcag tagtataaat aacgatatca tctcgttcat acccttcgtt
    29161 acgcaaccgt tcaattactg ataatgcttc ttccctcgta ctatagcttc cgatgaccgt
    29221 taaatttttc tcttccatgt aaattcctcc tagcaaatag atttatgatt ggtatattgt
    29281 tacttataaa tttattctat aggagacgat tgttttctac aaatatttag attggaattt
    29341 gattaaaaca atttaattga aatttgaaaa catttatgtt aatgaaagtc atattctaat
    29401 aaaatgtcat ttgtttctat atgaattttt atgaaaaatt tcatataatt tccactcgtt
    29461 gtgatggttt aatttatcca atccatcaat aagcaaacaa cttatataaa aaaggattaa
    29521 ttctcaatat aattgctcct accatagaat aaatcttcat gtaaagtaat gaagtaccaa
    29581 ctatttctaa tacttcttta agaattatcc caacaatatc gttagacaaa ccattattga
    29641 ataaatggag agccaatcga ttctgaaaaa atgttttcta atttttcgga aataaaagaa
    29701 agagcctcct gccattagaa cgaaccaaat ccatttcatt gctcatgaag taaccacata
    29761 gctaaaaaga ggataaatca attaaaagat caaaagaatg actgcactaa taaaataact
    29821 aaaaatgtat cttcttactt catatctttt agctagtact tagttaaaaa tttgcttcct
    29881 aagatcaaga caaactgtct atcttcattt gatgaaatgc ggtaaaatat agaaaatgaa
    29941 cactattgga gggaatttgt aatggacagc aaaactagaa aacaactgca agaaaagtta
    30001 aagcaacgta cacacttgct cccttccgtc tttcaacagt ttttagaaga aaatcgttct
    30061 tccctttcat taggcagtcg ctatgaatac gcaaaagatt ttcatctgtt tactgattat
    30121 ttgagaaaag tattttctga agaagtggat gatcagctga ttatccattt agaaaaacaa
    30181 gattatcaga atttcttagc taaaatctat cgacatactc gttcgttcca aactacagcg
    30241 aaccaagaaa cggagcaatt gtttgaaaat agctattacg ggatttcaag gaagcaatat
    30301 tcattaaatc attttttacg atttttgtat caaaaaggac tgattgaaga agagatttct
    30361 cttttgggta caagtttacc ggaaacaacc aaagcagctc cgcgatatat cgatgcagaa
    30421 cttttcaaag attttgagca tttgtttatc ccgattgaag gctttcaaac aagcagagaa
    30481 gctagctttg aagaaaagaa tagaccgagg aaccttgcaa ttactgccat tcttctgtat
    30541 tgcggattaa agatccatga agtagtagaa cttaaacgta aagatgtatc tttgaccaag
    30601 cagattctta atctgaatag aaaagaaaac agactgaata aagtaccaat accaaaagaa
    30661 gcgatgccat ttctggagaa ctattttaag ttaacagcac aaaatacgga cgaaaattct
    30721 tttgtttttc gttcctcaca tgatcagcaa atttctcctc ggactattcg ccaaattttg
    30781 agcaaaatta cagtatataa aaacgtatcg ccagaactat gcagaaaaac atatcgttat
    30841 tatgcagaac tatatttaga tgatgctgac gcggtaaatt atttatgtgg taatcgatac
    30901 ctctcatccc attcttttca agatatctgg gaaaagatgg ttgatttttc ttatcacgaa
    30961 ttagcggcat tcgttcgggt ttcttccata taaaacaaat gtatgatctt tttttcacaa
    31021 taaactatgt tattatataa aggattatgt tacgattatc ttgaatttgt atattactta
    31081 acaaaagata atggagtgtg aatgatggag accataacgt attccccttt gaacttattt
    31141 acgaattttc aagaagctgc agctaacttt tccgagaccc cgattatctt tgatgagcca
    31201 ctttcagctt tcccagaatt aggacttgaa acaacttaca aaaaatgctg cgaaagtgtg
    31261 ttgaaacggg cttatcaatt ggctcatctt ggagtgaaat ccggagacaa agttattatt
    31321 tataagagtt ccgcttttga cacatacctt ctagctgttt ctgtgtctta ccttggtgca
    31381 gtgcctgtca tgacttctta tcatctacca acaacaacaa tggaagtatt tatcgatcgt
    31441 ttagaagacc ctttcatttt atttgatgat gagacaaaaa agcgagtgag agaaatttca
    31501 aatggaacaa aatcaaaaca gattcctatt ctttatttaa tggaacaacc tgctttgtct
    31561 gtttctcaat catttttaga caaaaatgaa atttcttata tgacccatac ttctggtaca
    31621 actggtatcc ctaaacttat ttgtcattct gctcattcta tgggatggcg gacgaaatgg
    31681 caaaaaacag ttttcacaaa aatttcggaa aagaaattaa tcgcttttca catctctcca
    31741 gtgcattcca gattcaatat tggtatctct tctttgatgt ccatgggatt tcctatgatg
    31801 cctttagcaa acgcacaaag ttcaaaagtt gttcatatgt tggaagctca ccggccaatt
    31861 gcactagaga cccatccgaa taactttgtt cagtggcgtt ttactgctaa ggagcatcca
    31921 gaagcttttt cgggtatccg atactatcac tccacttttg atgctataaa caaccagaca
    31981 atgaagacat ttttgcgaac gtcgatggca caggatgcgg tatttcttca agtatatggc
    32041 caaagtgaat gcggaccaat gattcttaag gctcatacac tagagagttt gaaaacaagt
    32101 gatgcaagag atatgggcgt tggactggaa gacatgacaa gcgctcggat tacagattca
    32161 gttgggaatg tcctacctga aaacacagat ggtcatatac aattactgtc taaaggacgg
    32221 gctttgactt attacaagga agatgcacgg ttccaggaaa atgtttatgg cgattggtgg
    32281 gatagcggag attacggttt tatggataag catggccacc tatttttgaa agatcggcaa
    32341 gtggatttga tcgaaacgat caatagtaca ttagctattg aagacttttt attagattcg
    32401 ttagattttt tggcagaagt agtgattgta agggataaaa atcattctcc acaaccgatt
    32461 attgctttag ctcctgataa agaaatggat tggaatcgat ggtgggagca agtccatgaa
    32521 cttcctcgct tgaacgtccc tatcatccgt gattttgatg caatcccgcg aacagctaca
    32581 atgaaagtgc aacggcttca aatcgagaaa gaattaaaat cacaaaagtc ctaactattt
    32641 aaggaaagaa agatccattt tggtctttct tttctttttg tttttttaaa ttggatgaga
    32701 taaaaatatg ccaattggct gtttttcttt tttgaaaatt tcgttatact cttattcatg
    32761 gaggaaaatg ttatgaaaag aatcttgaca attggcggtt ctgatccatt tgctggtgga
    32821 ggaatccaaa cagatttaaa aacttttgag aattttggtc tattcggact aagcgccttg
    32881 acttctatag gggcactaga tattaatgag cgatttatgc tggaatctat atctttggaa
    32941 ttgcttaaac gacagttagt ttcgattgat aaaatgactt cattagatgg tatcaaaatt
    33001 gggttattga attcaagtga agcaatcatg gttgttcgag acttcataaa aacaaaacaa
    33061 aatatcccaa tcattttaga ccctgttttg gcattcaaag aaactgaagc tgtgatcaat
    33121 caattgtata tttcgcagct catcgaagaa ctttttccac ttgttacgat gattacgcct
    33181 aatttggcag aagctgcttt actttctggt caagctcttc cctcttcgtt aacagaaatg
    33241 atccatctcg ctaaagaact cactgcttca ggaacaaaaa acattgtgat caaaggcggc
    33301 acaaggatgg ctggagatga agctgtggat cttctctatt caattgatca ttatgaggta
    33361 ttttctcata aaaaaatcga cactacaaca gtaaacgggg ccggttgtgc cttttcttct
    33421 gcaataacag cgaatcttgt gttaggggca gattttacca aagcagtagc agaaagtaaa
    33481 aaatttgttg cccattgtat ttctaatggc gtgctgatga acgatcatac cggaagtgtc
    33541 tggtctggcg gtattcaaaa aggaggaatg agaaatgaaa actaagcaat taacgatcta
    33601 tgctatgctg attgcgttga cagttgccct ttcattaaca attttgatcc ctgtaccggc
    33661 tacgaatgga tttgtgacat tatgcgaggc cgggatctat accgctgctt ttctttatgg
    33721 tccaacgggt ggattagttg ttggtgcttc cagtggtcta ttgatcgatt tgatttcagg
    33781 atatccgcag tgggcagttt tttcattagt gatccacggg ttacaaggat atgttgctgg
    33841 ttctttcagc aaatcttcta ctcgtatgtg gggattaggg cttttattag gaagcttagt
    33901 tatgatcatc ggctatggta tcgccggttg gattttatat gattggccgg ctggagtggc
    33961 ttctatacca ggaaacatct tccaaaatct gattggaatg ggacttgcct ttcctataac
    34021 tgcaagtctt caacgtattc aagcacggaa gcaatattaa ttgaaaaaga aggttttaga
    34081 aaatgaagtt gtcagaaaaa gaattaaaag accaattgac tgaaatagtt aatgacatat
    34141 tggctgaggc acacttgaaa aaaggtgatc tttttgtttt aggatgcagc acaagcgaag
    34201 tcgttggagg acatattgga aaaaattcca gtgcggaagt tggtcaatgg atcatccgta
    34261 cgttaaaaga gttgttggat cctaaagaaa ttgcgctagc tgtccaaggt tgtgaacatt
    34321 tgaaccgagc attagtagta gaacgaacag tggcagaagc aaaaaatttc gaaattgtct
    34381 ctgttgttcc tgcactacat gctggaggtg cttgttctgt tgctgctttt gaccagttca
    34441 atgatcctgt cgaagtggaa cacgtagttg gacaagccgg tatcgatatc ggtgacacat
    34501 caatcggtat gcatatcaag catgtccaag tacctgtcag acctcgtttg aaaacccttg
    34561 gacaagctca cgtgacagcc cttcgctctc gtcctaaata tatcggtggt cctagagcga
    34621 attattgaaa tgagtaattg acagagcaat ctgaaaaact tcctcgcaaa gtccaactaa
    34681 tgaactttaa cgaggaagtt ttacttgttt gttttatata accgagcttc ataaggtgct
    34741 agtatgcctt tagcttcaaa ttctccgcta gaggaatttg acaagagtaa tgtccaggac
    34801 tgatctgctt cttttggaag ttcatagtca gcaagtgatt ctgttaagtt taccataata
    34861 agatattgtt catcttctaa atatctttca tagacataaa gttgtggatg ttcatgaaga
    34921 tattctttat aagaaccgta tatcagacct ttattggttt gtctaatgcg gatcatgttc
    34981 ttatagaatt gcaggaccga ctctggttca tattcttgtt gttctacatt gatcgttttg
    35041 gtatttggat tagtgatcag ccaaggttca tgtgtagaaa aaccagcata ttgttccgaa
    35101 gtccattgca tgggtgtcct gctgttgtct ctcgttgttt cacgaatgat atccaatgct
    35161 tcttctcttg tcttaccttc cgcaagcagc cttttataaa gtcttttcga atctactgca
    35221 tcaacttgat cgatcgattc aaagggcata ttcgtcattc cgatttcctg tccttgataa
    35281 ataaatggcg taccttgcaa aaagaaatac atcatagcga ttgcttttgc agacatagcc
    35341 caaaaatcgg gacgagtatc tccaaacact gaaacggcac gaggaacatc atggttttcc
    35401 atatataaag cgttccatcc gataccatcc aatgaagttt gccaagcgct taatgcatgt
    35461 ttcaatttaa ggacatctaa ttgcccttct ttttcttgct gccagatatg gatatgatca
    35521 aattcgaata tcatatcgaa ataaccgtct ttccctaccc attgtggtcc ttcttctgca
    35581 gtgacaccac ttgcttctcc gacagtcatg atatcgtact cttcaaaaac atgtttcaaa
    35641 tcggtcaaat gttcttcgat tcctgagaca ttttgaaagg gtgagaaagg gttctctgtg
    35701 gcttttactg aatattcatc tttttttaca tgagaaatcg catctaaacg aaatccatct
    35761 atccctttat ccaaccacca tcggatcata ttgaatagtt cttctttcaa tttctcgctt
    35821 tcccaattta agtctggttg ttcttttgca aaaacatgca gataatactg tccagtttca
    35881 tgagaatact cccaagcaga accgccgaaa atcgatgtcc aattattcgg ttctttgcca
    35941 tctaccccgt cgacccaaat gtaataatcg cgatacgggc tttctttaga ttttttcgat
    36001 tcttcaaacc attgatgctg atccgaacta tggttgatga caagatccat aatcactttg
    36061 atccctaatt catgtgcttc ttttagcaat aaatcaaaat cttccatcgt tccgaatctc
    36121 tcttctatat cttgatagtc acttacgtca taaccattat cgacaaatgg agattgatag
    36181 atcggattga tccagataaa gccgatacca agatctttca aataattaag tttttcacgg
    36241 atcccattga tgtctccttg accatcgcca ttgctatctt tgaaactagc aggatagatt
    36301 tgatagccta ctgcttcttt ccaccatttt tgttcttgta tcatatcgtt tcactccttt
    36361 aacggtccct cttgattata accgattcta ttttagaagt atccttgtta ccggtaacta
    36421 gaaacgaaaa gctcaacgaa tctgctaagt gcgttcgttg agctttattt tatcatagat
    36481 agcgggcaat cagttgaatc atttgcttgg gaaattcatt caagtcagta atatctacaa
    36541 aacgactttc accgtagatc gacttgattt cttctttgtc ttggccaata gctgcggaga
    36601 caaataggat tccttgacgt tcatattctt tcaaaacaga ctggatatct tcttttgctt
    36661 ttttgcctgt ataatccggc aatgcttttg gctgaccgtc actaatatta agcaacagtt
    36721 tggttgttgc ggattgtctg tttaattttt ctgctgctaa acgaagtgca gcaccatcac
    36781 gattgttttg ccttggtttc atagtgacta atttttcatt taaccattga aatccgtctt
    36841 caaattcttt ataagaaaac aaagaagtct tttctcttga cgaacggtct gctgtatctc
    36901 cgtaaatcaa caatgggata tcgactttct tagaaaattc ggcaatggca atcgctgctt
    36961 tttttgccgc ttcgatccga tcatcccgta tcattgaccc agattcatcg atccgtacag
    37021 ctaccgctaa agaaggttgc tcatgtggcg ggttcttttt gtcaaaattt ctcaaatcac
    37081 catatgctac tttgcttgca ttgaaccgct gaccttcgta ttttcctttt tggtacgtct
    37141 ctgtttgttc attatccagt aactctaaaa tttgccggct aagtgattct actattggca
    37201 ttacttgcat gtgtagctca cgtgcttccg gcgtttttcc ttggaaagct ggtcggtgaa
    37261 cgatgagacc ttctttttca tgaatcgttt ccttcatgat ttgtcgcgct tctttattca
    37321 gttgcttctt gatttcctta tcatgtgctt ccatgtcaga agcagacatt tcttgagaat
    37381 catctcctgt ttgttgttct ttttgatttt ttccttcgat atcgctactg cgttcttttt
    37441 gcatttgagg aggaatctcg tttttttcta ttgtttcttc ttgtttctct tctccctcat
    37501 ttggttcttc agcagacaac gatttctttt catctaagcg cagaatttct tcagcatttt
    37561 cagtgatgtt tcgttcattt gcttcttcgg cgctaatggt tctttctttt gtatgctcag
    37621 attgaggatg accccatttt tcttttctca atatgtcctg aagttccagt agcaatccac
    37681 tgtcagacaa agcttgttca agaattgcca attcatctga atcagtggat aatttataca
    37741 aaacttttgg ataaatcgac tggagtaaat cttgtccttg cttataggta tttgcccaat
    37801 aaaaataaga acgcattcct gctacacctt tgatggcgtt agcttttgca gtttcatcca
    37861 acaaacggat aatctctgcc atctttaaga gaagttcttg tttttggatc atagttttcg
    37921 ccatcgcacg ttctgccatg acttctaagg caggaagatc cattttttcc gcatgttgca
    37981 tccgatctct taaagattca tttaatgggc gatttccttg gtaatttcga tttgtagtga
    38041 tcacaatgac gcaatctgga tggcgacgaa tgattcctgt cggtaaattc aacatcccat
    38101 ttggttcgag cgctgagtta agtgccacaa gaacagaggc atctcggatg acagtcggct
    38161 cttgaatctc taataaatag cctttttcaa tcgctcgaac gatttcagaa gggtaatagc
    38221 tgtaatggat tgcatctgga taatcagctt cgatcctttg aactagctct gctaattttt
    38281 ctttagcaga aaaaggatca atgccgtaat ggttctggat cagtgtcagt acagcttcaa
    38341 gtgtatctgt ttggtaaata gcttctagta attcttggtc ttgttcctta ttagaatcta
    38401 ctacaggtaa taaggctccg aaaacatctg atttgtccat atctgcaaaa caggtcactt
    38461 ttgtataggg aagttgcaga tcagcagata gtgctttggc taattgtgtc ttccccgacc
    38521 cggcatctcc ttccaaaagt acgttactga ttttcatttc aggatcttgc caattccctt
    38581 tgatttcgga ggcgatccgc cattcttctg cacttgtccg atggcttttc ggtttcttcc
    38641 agatcatttg cttttctagc cgtgaaaagg ttcggttttc tgttagtgag aaattctcca
    38701 tgcttctctt ccccttttga tcatctctgt ctattaatat cctagctcat ctaaaagacg
    38761 ttttaaaata cggaatcgtt ccccaatcag ttctccgtct ttttgtagtg tatcgtaata
    38821 caaaatgatg tcttcatcga tctttggatt tttggtatct acttctaccg tcattcggtt
    38881 cccttgtaat ccaatgatgt ctacgactgg attttctgga tcataaaatt tttcataacg
    38941 ataagcatct tggaagtata aacttccttg cgcagctaag atcgctaaat ctaaaatacg
    39001 tgtgattggt aattcttccg actgtcgtga ccatttttct ccagtatgtc gccatacctt
    39061 gcctgagata tcgacttttc cacgatcatt ccactgagca aggcccagtg aaagcccttt
    39121 tgcatccata tttaatgcat ttcttccatc aactttatca tagtcttcta caacgattac
    39181 tggtttatgt tttaaatttg ttggtatttc cattatttta actcctcatc cttttatttt
    39241 tagtaaataa gtaaattagt agtttactca tttactaaac tatatgcgac attatagagg
    39301 attgaagtga ggattgcaaa gaaaaagagc ttcattgata gaaataaaaa caccatctcg
    39361 ttcttttaca cgctcattag taacatgtag aaagtccaag atgatgttgg aat
//
LOCUS       DACEEZ010000016        39096 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20313_2017
            SAMN12639966-rid9879223.denovo.016, whole genome shotgun sequence.
ACCESSION   DACEEZ010000016 DACEEZ010000000
VERSION     DACEEZ010000016.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639966
            Sequence Read Archive: SRR10023158
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 39096)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 39096)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741683.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741683.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:44:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,016
            CDSs (total)                      :: 2,962
            Genes (coding)                    :: 2,889
            CDSs (with protein)               :: 2,889
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 36 of 73
            Pseudo Genes (incomplete)         :: 29 of 73
            Pseudo Genes (internal stop)      :: 18 of 73
            Pseudo Genes (multiple problems)  :: 8 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..39096
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639966-rid9879223.denovo.016"
                     /isolate="E20313_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            119..1045
                     /gene="cysK"
                     /locus_tag="IWO89_000908"
     CDS             119..1045
                     /gene="cysK"
                     /locus_tag="IWO89_000908"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase A"
                     /protein_id="HAQ0455681.1"
                     /translation="MTAIYNSVTELIGKTPIVKLNKIVPEDSADVFVKLEFFNPGGSV
                     KDRIALSMIEKAEHDGLLKPGDTIIEPTSGNTGIGLSMVGVAKGYKVIIVMPETMSIE
                     RRLLMKGYGAELILTPGADGISGSIREAERLAKENGYFLPLQFENEANPLVHEKTTGP
                     EIHQAFGVNGLDAFVAGIGTGGTITGAGRELKRVYPKIELIGVEPAESAILEGKEAGP
                     HKIQGIGTGFVPKTLDTSVYDKVLSISGDEAMETAREVGRKEGILVGISSGAAIAAAL
                     KVAKELGKGKKVLAVVPDNGERYLSTALYQEI"
     gene            1281..1727
                     /locus_tag="IWO89_000909"
     CDS             1281..1727
                     /locus_tag="IWO89_000909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor"
                     /protein_id="HAQ0455682.1"
                     /translation="MENKLVEEVIEKLKKENIRITPQRYAVLEYLIEHHSHPTADEIY
                     KALEHRFPNMSVATVYNNLRLFTSIGFVQEMKYGDASSRFDFSSKRHYHVICENCGKI
                     VDFHYPGLEDIEMAASRLTDFEINEHRLELYGLCPDCKESAKKAQM"
     gene            2000..3352
                     /locus_tag="IWO89_000910"
     CDS             2000..3352
                     /locus_tag="IWO89_000910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH dehydrogenase"
                     /protein_id="HAQ0455683.1"
                     /translation="MKVVVIGCTHAGTAAVKSILKNHPEAEVTVYERNDNISFLSCGI
                     ALYVGGVVKDPAGLFYSNPEELSSLGATVKMEHDVTDVDTENKTVTAKDLKTGETETV
                     SYDKLVMTTGSWPIIPPISGIDAKNVLLCKNYNQANEIIAQAKEAKRVVIVGGGYIGI
                     ELVEAFVESGKQVTLIDGLDRILNKYLDKPFTDILEKELTDRGVTLALGENVTEFITD
                     EEGKVKQVATPTDTFDTDMVILCVGFRPNTKLIEGKVDTLPNGAIKVNEYMQTSNPDI
                     FAAGDSAVVNYNPSGTQNYIPLATNAVRQGLLVGNNLTDHKMAYRGTQGTSGLYLFGW
                     TIGSTGVTKESATLNDLEVQATVFEDNYRPEFMPTTEKVMMELVYEKGTNRIVGAQFM
                     SKYDITQSANTMSLAVQNKMTVEDLALSDFFFQPHFDRPWNYLNLLAQAALGEIETAQ
                     "
     gene            3523..3810
                     /locus_tag="IWO89_000911"
     CDS             3523..3810
                     /locus_tag="IWO89_000911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0455684.1"
                     /translation="MNKEYFVQQDAFLEDYKSASKKLTINIDELADQVLSQLNETQEE
                     YYTMPANDAKDNVSHRFPFSRRIYSDENDGTLNTEYTYEGQPYVLETDDDL"
     gene            4036..4479
                     /locus_tag="IWO89_000912"
     CDS             4036..4479
                     /locus_tag="IWO89_000912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HAQ0455685.1"
                     /translation="MDKRQLLIEEYLDLANEIIHRNKPEMEKEFAGFTLNELEVIEHI
                     GKIPDPNVTKLANASYMTRGAISKLTKKLIARGIINTYQSEENKKEIYFQLTPIGQKI
                     RNRHEQLHQKWVQRDATVFENMSKEEFDTVFRFIGRYREFIRSSK"
     gene            complement(4485..5669)
                     /locus_tag="IWO89_000913"
     CDS             complement(4485..5669)
                     /locus_tag="IWO89_000913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance MFS efflux pump"
                     /protein_id="HAQ0455686.1"
                     /translation="MYSFTNKHSKVFGYVSVLFTGLGFTIVSPVLPFLTLPYSHSIHQ
                     QAFYITLLMSVYALAAFLSAPILGSLSDHFGRHPILIISLLGSSIGYLLFGLGNSIWM
                     LFLGRIIEGLTAGEISTLYAYFADITEPNERTKVFGWMGALVGIGTTLGPIIGGLLAE
                     LGNSVPIFIGALFTFLNAVYGYTFMLESLPIKKRSVDLSFSHVRPFHQLQQLFKISSV
                     IPLLTAGFAVWLAAGSLQSIFSQFSIDAFQWKAGLVGLSFSLIGILDTVSQLFIMPRL
                     LKKFSEQQITRIGMFSEILAYLFITLSGILLLPILFLFGIICYGFGDSIFTPVFNGQL
                     SNSVSDNQQGLVMGGTQSIQSLSRVIGPLIAGQLYAAAPFLPTVFGFLLMIFAYYHYN
                     KK"
     gene            5937..6635
                     /locus_tag="IWO89_000914"
     CDS             5937..6635
                     /locus_tag="IWO89_000914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaD domain protein"
                     /protein_id="HAQ0455687.1"
                     /translation="MLNLENYLSAGQTVISNLLLKNYHRIGLQSEEFLFVLQLHMAQL
                     EGDTFPDLQMIAMDMGIKQDQIFQILDRLVTNGFIKIETTIVNGKKADRYNLYPIYDL
                     LGEYLKTQEKKYEEKTQEKEIQSVYQLFEQEFGRPLSSIEFQRIGQWLEEDHYQPEIL
                     KLALREAVLNQAYSFNYVDRILLSWERKNLRTKQQVEEDQKRRKQQLLQREVEQSAQQ
                     EELPKVSLKNWLEE"
     gene            6642..7319
                     /gene="nth"
                     /locus_tag="IWO89_000915"
     CDS             6642..7319
                     /gene="nth"
                     /locus_tag="IWO89_000915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HAQ0455688.1"
                     /translation="MLSKQKTMEALETMYGMFPEAHGELKHNNPFELLIAVILSAQAT
                     DVSVNKATPDLFASFPTPDALAEASIDEIILKIKTIGLYRNKAKNIKACAQQLIERFD
                     GQVPTSREELMSLPGVGRKTANVVLGDAFGIPAIAVDTHVERVSKRLRICKLDATVME
                     VEETLMRKVPQELWVKTHHTLIFFGRYHCTARNPKCEVCPLLSICQDGKNRMRLKEKA
                     LKKKPRL"
     gene            complement(7445..7978)
                     /locus_tag="IWO89_000916"
     CDS             complement(7445..7978)
                     /locus_tag="IWO89_000916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0455689.1"
                     /translation="MVLNNFLKEGFILSYLIVCLIIIICLHHLFLSKTIPRYSKNKKA
                     EKFCLLLNKFTLIAPILAFIIFSVLLSTTLKGKFMERSSHAMILTFLWLLFTRIYIFL
                     MSLKPPKSISLCLVINGIFLLSLIIFITPLDRYVTYLYNPLEYWTYFIGILEGIIFYI
                     GYFPNKNNNFYFYRNKL"
     gene            complement(8275..10656)
                     /locus_tag="IWO89_000917"
     CDS             complement(8275..10656)
                     /locus_tag="IWO89_000917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PBP1A family penicillin-binding protein"
                     /protein_id="HAQ0455690.1"
                     /translation="MANEQTRSSRRQKQPTPKKSVKKNSGKGSGKSSGTHKKGLFIKI
                     LLGILSFFCILFLAGVGLFWYYAKDAPELTDKKLDATVSSKLYTQDGELFEDLGAEKR
                     EKISANELPKTLEDAIVSVEDRRFYKHIGVDPIRIIGSALSNFTSGGLQGGSTLTQQL
                     IKLSFFSTSAEDQTLKRKAQEAWMAVRLEQKKSKQEILTYYVNKVYMSNGLYGMETAS
                     EMYFGKKLSELSLPQTALLAGMPQAPSAYDPYVYPDQAKKRRDTVLYTMLQNEKISQT
                     EYDQAVNVPVTDGLQELTQSDDNTKIVDNYVKEVINEVQEKTDKNVYTDGLEIYTNLD
                     LDAQKKLYDIVNTDQYVSYPDDEMQVASTLIDTNTGKVKAQIGGRHIAEDVTLGNNLA
                     VNTSRDFGSTMKPVTDYGPAFEYLKYSTGKTITDAPYNYEGTSTPVGNWDNQYMGTIT
                     LRQALYLSRNVPAVKLFNEVGSDKVASFLKNLGIEYSTIHQSNAISSNTEEQDGTKYG
                     ASSLKMAAAYAAFANGGTYYKPQYVNKIVFQDGTEETYEPDGKTAMSPETAYMITDIL
                     KDTITEGTGTNAQIAGLYQAGKTGTSNYTDDEYAKLGISSGVYPDILFAGYTPNYSIS
                     VWTGYNKKMTPVTSESSHVASDVYRELMQYVSANVTNTDWEMPSGLIRVGGELYYKDQ
                     YTARSNAITPSTTIPSSSYVQTPGSSTTETTTQSSSSTSQSESTAESSKESTTAETSE
                     PASSTTVPSSSSEESSTPSSSAPPASSSEPATSGADAANDHTPSSSTSASGNR"
     gene            complement(10646..11293)
                     /gene="recU"
                     /locus_tag="IWO89_000918"
     CDS             complement(10646..11293)
                     /gene="recU"
                     /locus_tag="IWO89_000918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RecU"
                     /protein_id="HAQ0455691.1"
                     /translation="MVLRYPNGQPYYKNASPVEKIQEKRNQITEFGNRGMRFEEAINE
                     SNKYYLSRGLAVIHKKPTPIQIVKVDYPKRSAAVIKEAYFTQASTTDYNGVYRGFYLD
                     FEAKETKNKTSFPFKNFHAHQIEHMKACLEQNGVCFVLLWFSSLKRCFYLNSVHLIAY
                     WDAQKEKGRKSLPLSLIEELGIEIKTGISPRIPYLDAVDFYLSTLSIEGESKNGK"
     gene            11357..11896
                     /locus_tag="IWO89_000919"
     CDS             11357..11896
                     /locus_tag="IWO89_000919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1273 domain-containing protein"
                     /protein_id="HAQ0455692.1"
                     /translation="MDKSKVIYVSGYRSFELGVFKENDPKIQVVKNVLKKELHQLAEE
                     GLEWVLVSGNLGVELWAVQIVAELKEEYPELSLGILYPFSDFGNNWNEGNQEKKQLAE
                     QLADYVEAVSHQPYQSPSQLKNHTRFILEHTVGCLLIYDEEFPGKTKYFFEDAQNYQA
                     DHPYEIRLISMDDLQNFSE"
     gene            11986..12405
                     /gene="gpsB"
                     /locus_tag="IWO89_000920"
     CDS             11986..12405
                     /gene="gpsB"
                     /locus_tag="IWO89_000920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division regulator GpsB"
                     /protein_id="HAQ0455693.1"
                     /translation="MANLVYSPKDILQQEFKTKMRGYDPVEVDEFLDNIIKDYEAYSK
                     ELLALQEENDRLSAKVAQLSKTQGAAQTRVQQTEAPKSAAVTNFDILKRLSNLEREVF
                     GKKLDQQASAVKPAQPNPNNYTNADTSLDDNEKTRQF"
     gene            12427..12794
                     /gene="rnpB"
                     /locus_tag="IWO89_000921"
     ncRNA           12427..12794
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="IWO89_000921"
                     /product="RNase P RNA component class B"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00011"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00011"
     gene            13003..14169
                     /locus_tag="IWO89_000922"
     CDS             13003..14169
                     /locus_tag="IWO89_000922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent RNA methyltransferase"
                     /protein_id="HAQ0455694.1"
                     /translation="MEKNRKFNLVATAASGLEALVGKELRDLGIECQVENGRARFEGT
                     METIATANLWLRTADRVKIVVGEFDAYTFDELFENVKALPWEDFLPLDAAFPVAGKSI
                     KSKLYSTPDCQAITKKAIVERLRTYYHRPASVPLTETGAHFQLEVALLKDHVMVTLDT
                     TGPSLFKRGYRLEKGGAPLKENMAAALIMLTNWRKDRPFYDPVCGSGTLCIEAALIGH
                     NIAPGFNREFACESWDWFSQEIMENVRSAAEEKADYDIELDITGSDINGRMIEIAKAN
                     AEEIGLGSSITFKQQAVKDFKTDKEYGVIVANPPYGERLGEEETVRKLYKEMGDVFRP
                     LKTWSKYILTSDLAFEEFYGQKATKKRKLYNGALRTDLFQYWGTRPPRKKEKDE"
     gene            14215..15711
                     /locus_tag="IWO89_000923"
     CDS             14215..15711
                     /locus_tag="IWO89_000923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase M32"
                     /protein_id="HAQ0455695.1"
                     /translation="MKEKEFLQEVKEINLLQQALGILDWDTQTGMPEKASAYRSEVDS
                     YLYSIYFSKKIGPKIKEAIRYFSEHPEELSEVGTAVFAKVKEEYDLEHRVPEDLMRKY
                     TAAVSSAHGQWQKARETQQFSDFQHALNQNIELTKQLIPYWRKDEKTNYDVLLNQYEP
                     GMTVEILDKVFDQLKEGILSIRRTLQEKGTEPETDFLNRRMTKKQQKRFVVKVIEQLG
                     YDFSRGRLDDTVHPFMTGINPNDARITTRWNETDFKMAVFGVIHEAGHGMYEQDIDDK
                     YAYTPVYEGTSMGIHESQSLFNEIIIGSSRSFWKKQYPFFQECAEGTFDDIPFDKFYR
                     SLNHTKSSLVRIEADSLTYPLHIIIRYEIEKMIFNEGVDVAELPEIWNQKYEEYLGIR
                     PTNDVEGILQDVHWSGASFGYFPSYALGYMYAAQLLHSLQQEIDVENVLASDDYSPIK
                     NWMSEHIHQYGASRKPNQLIMDATHEPLNPQYLIDYMKDLYFNVYQVK"
     gene            15792..16475
                     /locus_tag="IWO89_000924"
     CDS             15792..16475
                     /locus_tag="IWO89_000924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HAQ0455696.1"
                     /translation="MDKLQVIADYSMDQLAHDQTGHGSDHTKRVVKLAEKILETEPQA
                     DRFVTLAAAYLHDTIDDKVVKNENEAKQQLRVFLRSLPIVEEQISMIFSIIENMSFSK
                     NLSEAAELSLEGKIVQDADRIEALGAIGILRTAYFGGGHGHPIFDPELYPQTFKDKEN
                     YRKGTTVINHFYEKLFLLSDKMNTDYGYEEAKRRETFMREFLEEFFLEWSIDDENFTP
                     DNWIKNNKK"
     gene            17120..18310
                     /locus_tag="IWO89_000925"
     CDS             17120..18310
                     /locus_tag="IWO89_000925"
                     /EC_number="2.3.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine C-acetyltransferase"
                     /protein_id="HAQ0455697.1"
                     /translation="MSSKELTMYLEKELDQLKEKGLYNTIDILESENGACIIVDGKKM
                     INLASNNYLGFANREELKKACIEATETYGVGAGAVRTINGSLKIHQQLEEKIAEFKGT
                     EAAIAFQSGFNCNMGAISAVMTKEDAILSDELNHASIIDGCRLSGAKIIRIKHQDMKD
                     LEKKAKEAIESKKYKKIMYITDGVFSMDGDIARLPEIIPIVEKYGLITYVDDAHGSGV
                     TGKGAGTVKHFGLSDKIDMQMGTLSKAIGVVGGYVAGSKTLIDWLKARSRPFLFSTSL
                     TPGAAAAALASITLMQEHPELVEKVWENANYFKEELKKVGYNIGMSETPITPVILGDE
                     KVTQTFSKKLIEHGIYAKPIVYPTVPLGTGRIRNMPTAEHTREMLDEAVAVYQKIGKE
                     MEII"
     gene            18412..19365
                     /locus_tag="IWO89_000926"
     CDS             18412..19365
                     /locus_tag="IWO89_000926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonine 3-dehydrogenase"
                     /protein_id="HAQ0455698.1"
                     /translation="MKKVLVTGCLGQIGSELITRLRQELGSENVIATDIRMPEVIEKN
                     DIFEILDVMDYEKMRSIAEQYQVDTLIHLAALLSAVAEAKPQLAWNLNVTGLVNALEV
                     AREFQLKFFTPSSIGAFGPNTPKDHTPQDTLQRPTTMYGVTKVAGELLCDYYYVKYGV
                     DTRGVRFPGLISYATLPGGGTTDYAVDIYYSAIKEGKYTSFIDQGTSMDMMYMPDAID
                     AIIKLMNADPDRLVHRNAFNISAMSFDPEEIKASIQKIIPDFEMDYDVDPVRQSIAES
                     WPNSLDVSCAQEEWGFSPKYDLDVMTKDMLEKLKEKLVETV"
     gene            19434..20783
                     /locus_tag="IWO89_000927"
     CDS             19434..20783
                     /locus_tag="IWO89_000927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAQ0455699.1"
                     /translation="MEEYQATPVKEVKIENELKRTMGFFTALSTVMGTVIGAGVFFKA
                     ASVAEVTGSASLHMFSWFLGGMISVCAGLTGAELAAAIPETGGMIKYIERIYGNTAAF
                     LLGWAQVVIYFPANVAALSIIFGTQFVNLFGLSQSMIVPVAVTAAVSILLINFLGSKA
                     GGAFQSITLVCKLIPLFVIVIFGLFRQEGVDFQLFPIQAGENLSFFSALGAGLLATMF
                     AYDGWIHVGNISGELKKPAKDLPKAISLGIIGIMIVYLLVNAVFLRTASIDGVAGNSN
                     AASDVAKMIFGGFGGRLVTVGILISVYGTINGYTLTGMRLPYVMAKENNLPFSKLFAK
                     LHDKTKVPVAAGILELVIAIGMMMIGGFDTLTDMLIFVIWIFYTMVFVGVILLRKKEP
                     DLFRPYKVPMYPFIPLVAIIGGTFIVSSTLITQTFLASMGIVLTLAGIPIYLYLKRR"
     gene            complement(20966..21289)
                     /locus_tag="IWO89_000928"
     CDS             complement(20966..21289)
                     /locus_tag="IWO89_000928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0455700.1"
                     /translation="MLNMYFVFGVPIFLLFLYATIAYVRKRTTIHYLGFILLIISGFM
                     LVFNLQTWQQALLEMDKMTPHALSKVLGYPVYLIWLPIFISGCLVLLNIYRGVRRIVQ
                     LRKSK"
     gene            21418..22581
                     /locus_tag="IWO89_000929"
     CDS             21418..22581
                     /locus_tag="IWO89_000929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0455701.1"
                     /translation="MASYIEPIRFALIVFPFLALAISSVFFIYEYRKYGTFLVTRAVI
                     LYSFVFYLLCAYFLVILPLPAKETVAQMTGPKMELHLFASWQHFRDNTVLVLSEPSTY
                     LPAMKQSVFLEPAFNVLLLIPFGIYLRYYFRLSFMKTMIASFCLSLFFELTQLSGLYF
                     IYPRPYRLFDVNDLVTNTLGGIIGFVVTPLFTFMLPTRRRMDELSYEKGKTVSLTRRL
                     VAYLIDWTFLSLINLVAAIFFKIAFDSGAVTSHIWWLLLEVGVYFMIVPFITNGWTLG
                     KKIVRIRLVEEDRERISLKALMIRYGYLYFLFYGLSWLGQLLQQIMNSTTNTVLQMSA
                     VFISLGVLLLQGIFILNILLSFLRKKRILFYEKASHTYTVSTIGQTKDDDSRN"
     gene            22651..24000
                     /locus_tag="IWO89_000930"
     CDS             22651..24000
                     /locus_tag="IWO89_000930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HAQ0455702.1"
                     /translation="MNNFDIFRFKPFINEALTAKGFKQPTEVQERLIPLIAKGKNIIG
                     QSQTGTGKTHTFLLPLFEKINAEKEEVQVVITAPSRELAAQIYEAATQIAKFSTPEIR
                     VSNFVGGTDKQRQLNRLHHQQPQVVVGTPGRILDMMNEQALKIHTAFAFVVDEADMTL
                     DMGFLNEVDQIASRLPEKLQFLVFSATIPEKLRPFLRKYMENPIIEEIKPKAVISEDI
                     ENWLISTKGKDENQMIYQLLTIGHPYLAIVFANTKQHVDEIADYLKNQGLKVAKIHGD
                     ITPRERKRVMRQVQNLEYQYVVATDLAARGIDIEGVSHVINAEIPEDLDFFIHRVGRT
                     GRNGLKGTAITLYSPNDEQAIDAIEKLGVSFEPKEIKNGEIVKTYDRNRRAKREKSKD
                     VLDPTLIGLVKKKKKKIKPGYKKKIDWAIAEKNKKERKIERRQQSRTARKNKKNSHK"
     gene            complement(24045..24476)
                     /locus_tag="IWO89_000931"
     CDS             complement(24045..24476)
                     /locus_tag="IWO89_000931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAQ0455703.1"
                     /translation="MKKIVGADEWIRAASCFLRYQVFVLEQGIPAELEFDGKDIPQQT
                     YYLFLDQKRPVATLRYEKKTPTCFKPERFCVAKEYRGKGLGGQLLEAAEKRAVSEGCQ
                     TSYVVAEMTAVDFYEKQGYHTCTEPFIEDEVLCIGMKKSLA"
     gene            24864..27506
                     /gene="alaS"
                     /locus_tag="IWO89_000932"
     CDS             24864..27506
                     /gene="alaS"
                     /locus_tag="IWO89_000932"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HAQ0455704.1"
                     /translation="MKQLSSAQVRQMFLDFFQSKGHTIEPSASLIPVNDPTLLWINSG
                     VATLKKYFDGSVVPENPRITNAQKSIRTNDIENVGKTARHHTMFEMLGNFSIGDYFKN
                     EAIHWAWEFLTDPKWMAFDPEKLYVTVYPKDTEAKRIWHEEVGLPLDHIIDVEDNFWD
                     IGAGPCGPDTEIFYDRGEAYNDLAEDDPENYPGGENERYLEIWNLVFSEFNHKPDNTF
                     EPLPHKNIDTGMGLERMVSIVQDASTNFETDLFMPIIHAVEKLSKQVKYGENPVTDIS
                     YKVIADHIRALSFAIGDGALPSNEGRGYVLRRLLRRAVMHGKKLGIEQAFLYRLVPVV
                     GEIMVSYYPEVLQQKEFIEKVIRTEEERFNETINEGLEILNQVIQEVKNENGTTLAGK
                     DIFKLYDTYGFPVELTEEVAEDEGLKVDHAGFEKEMDAQRERARSARSTEKSMGVQSA
                     LLTDIKVESKFIGYTEQESDSQLLVIVKGESLLNEVAEGEAQMIFDQTPFYAEMGGQV
                     ADQGWIMNEVGEIVAVVTDVQKAPNGQFMHTVEVKAPMSEGQTYHLLIDQSMRNRIIK
                     NHTATHLLHKALKEVLGEHANQAGSLVAPGHLRFDFTHFGQITSDELAEMERIVNEKI
                     WEAIPVETIETDIDTAKNMGAMALFGEKYGHDVRVVNIGGWSIELCGGTHVANTENIG
                     IFKIVSESGIGAGVRRIEAVTSKEAYELLEEEEKQLKQVATIVKSPQLKEVVSKAEQL
                     QQQVRELQKENEQLASKLANQQAGDVFKDPETINGKTIIAAQVKVKDMNQLRQLADQW
                     KQKNISDILVLANEQEGKVSLLAAMTKESNEAGLKAGDLIKAIAPKVGGGGGGRPDMA
                     QAGGKNPAGIPDALKAAKEWVAQQ"
     gene            27706..28302
                     /locus_tag="IWO89_000933"
     CDS             27706..28302
                     /locus_tag="IWO89_000933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HAQ0455705.1"
                     /translation="MNSRNKTFRLVLRAILLAIIIVQAMVPWLGFIPLGFISLTIIHI
                     TVIIAAVVLGPKDGMVIGLFWGIATIVRAYAMPTTPFDTLVFTNPIISVVPRVLVGLV
                     AGLVFHWIYQKNKSITISSAFAGVLGSLVNTVLVLGFMGLFYTGATADAYGVDSALLF
                     KTLAGVAAINGIPEAIGAGIITPLLAKALFAATPLKPE"
     gene            28424..29572
                     /locus_tag="IWO89_000934"
     CDS             28424..29572
                     /locus_tag="IWO89_000934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation transporter"
                     /protein_id="HAQ0455706.1"
                     /translation="MAKKQEEKKKQIPQQRTKKGVLAGILGLVTNLILFVSKLMIGLF
                     SGSVSIMADAVNSLSDTASSILTLIGFKIAAKPADQEHPYGHERFEYISGLFVSIIIT
                     YVGFQFLESSAEKIIHPENVSLTPIVFFVLIISIFLKFAQGKMYQVIAQSIDSETLRA
                     TATDSYNDVFTTLAVLLSAAIEWVTGWRIDGYIGFALALYILYSGLTMLRNFVYELLG
                     SRPTPAEIEEMEEHLNACDDILGYHDLLVHNYGPNKRFASVHIEVDESMNLNVAHKII
                     DLIEKDFKKSLDVELVCHLDPMPVDNEKYYNILRQLKQILLETGTGLNIHDFRVIHQG
                     KTLQFDVVVPEKFQFVDTELERILRSQIEEKIGDYQLEITFDHNYLLY"
     gene            complement(29610..30023)
                     /locus_tag="IWO89_000935"
     CDS             complement(29610..30023)
                     /locus_tag="IWO89_000935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HI family protein"
                     /protein_id="HAQ0455707.1"
                     /translation="MLKVYIDASTKGNPGPSGGGILIVYQGKQEQLSIPLSIGTNHQA
                     EFEVFLKTLEFLKKNDLQNETIFCFSDSKTLVSTVDKNATKNELFLPYLQRIQELLSE
                     FSLLILQWLPENKNKGADNLARQALHKQLKEKKTL"
     gene            30102..30533
                     /locus_tag="IWO89_000936"
     CDS             30102..30533
                     /locus_tag="IWO89_000936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EbsA family protein"
                     /protein_id="HAQ0455708.1"
                     /translation="MKKNIRWQPELAHTIIYWSLTLIILFFSLILSLENTGPYWKSNF
                     VLGIFLVFLFLGLRRSFILKSNSVIIRYAAFWKDREISVGQIRSLTPTNTGVQMMLQD
                     EKAPSVFMMRKKDQVVLFDHFEKNGPKSESIPYPYTKKRKS"
     gene            complement(30632..30865)
                     /locus_tag="IWO89_000937"
     CDS             complement(30632..30865)
                     /locus_tag="IWO89_000937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock domain-containing protein"
                     /protein_id="HAQ0455709.1"
                     /translation="MTTGIVKWFDNKKGYGFISYDDTEEIFVHFTAIEEEGFKTLEEN
                     QVVEFEIIEGNRGTQAAHVKKGNVQTTDTSMHE"
     gene            31048..32718
                     /locus_tag="IWO89_000938"
     CDS             31048..32718
                     /locus_tag="IWO89_000938"
                     /EC_number="6.3.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate--tetrahydrofolate ligase"
                     /protein_id="HAQ0455710.1"
                     /translation="MKNDLQISQETELEPIVEVASKLDLKEDDLELYGKYKAKINFSG
                     INRSKDNAEGHLILVTSINPTPAGEGKSTITVGLGDALNKINKKSVIALREPSLGPVM
                     GIKGGAAGGGYAQVLPMEDINLHFTGDMHAITTANNALSALLDNHIHQGNELNIDARR
                     VIWKRVVDLNDRELRKVIVGLGGPIQGVPREDGFDITVASEIMAILCLAADLEDLKAR
                     LARIVVGYTFDRQPVTAGDLKAEGALALLLKDAIKPNLVQTIYGTPAFVHGGPFANIA
                     HGCNSILATKTALRLADYVVTEAGFGADLGGEKFLDIKVPNLKKAPDAVVIVATVRAL
                     KMHGGMKKDELKNENLDALKVGFANLKRHIRNMEQYQLPVIVAINEFVTDTDNELTLL
                     EHLCENQGILAKRASVWADGAEGGVDLAKAVVRLIDRKEANYKPLYRLEETIQEKTET
                     IVKKIYGGNGVVFSKKAQKQIEEFTKNGWDKLPICMAKTQYSFSDDPSLLGAPEDFTI
                     TIREIIPKLGAGFLVALTGDVMTMPGLPKKPAALNMDVTNDGEVLGLF"
     gene            32865..33251
                     /locus_tag="IWO89_000939"
     CDS             32865..33251
                     /locus_tag="IWO89_000939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0455711.1"
                     /translation="MKTTFIRKTGFYGMGSSIQLRINNEKRSVLSHEQKITLDLEVPF
                     TMQVTFYWLKSPVYTVKEAKPAYVITMNYLILQIYPFLFLATGLGAVFLQNLFFSFFA
                     VIVLIAFFFYIKNRAYLIKEENYGKL"
     gene            33238..33936
                     /locus_tag="IWO89_000940"
     CDS             33238..33936
                     /locus_tag="IWO89_000940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HAQ0455712.1"
                     /translation="MANSDLFLISFNRIEKWMRDEMGNARNMGFTELVRRLAQRKQLM
                     IRKYEDDLLQLAQLRNAIVHDRIAVDFVIAEPNDWTVKRIQLIEQELIQPETVLPRFA
                     KRVTGFEQDLPLLELLKIVAEKRYSQFPLYNKGKFVALITLRNIGFWLAKESQKGPVD
                     LTNKRALDLIIQNGKYTNYHFVPAKTHIYEVEAMFREQGTLEAVLITKDGNPDGNLLG
                     IVRPRDIYKQIEKD"
     gene            33941..34405
                     /locus_tag="IWO89_000941"
     CDS             33941..34405
                     /locus_tag="IWO89_000941"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HAQ0455713.1"
                     /translation="MLIIYWIFSALLVGVDQLVKWWITDNLALGETKNLIPGILSLNH
                     IRNTGAAWSMLEGKMWFFTIVTLIAVVVILTLMIKNREKGNRWFMIGLSLILAGAIGN
                     FIDRIRLGYVVDMFQTDFMSFPIFNVADVTLVCGVICMLIYIILDEKDQRKK"
     gene            34402..35313
                     /locus_tag="IWO89_000942"
     CDS             34402..35313
                     /locus_tag="IWO89_000942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HAQ0455714.1"
                     /translation="MTELKTTIHHESGRIDKVLTNLFTDYSRSQIQLWLKNGAVSVNG
                     QPVKANYKVKQGDEITVLIPEPETLDVVPENIPLTIVYEDEDVAVINKPQGMVVHPSA
                     GHTHGTMVNALMYHIKDLSTINGVIRPGIVHRIDKDTSGLLMVAKNDQAHESLAQQLK
                     DKTSLRKYVALVHGEIPHEKGTINAPIGRSKENRKMQAIREDGKSAVTHFTVLERFEG
                     FTLVELKLETGRTHQIRVHMKYIGYPLAGDPVYGPKKTLKGNGQFLHAQMLGFTHPTT
                     NEKMIFEAPLPEVFEKTLEKLRKNIAF"
     gene            35548..36087
                     /gene="pyrR"
                     /locus_tag="IWO89_000943"
     CDS             35548..36087
                     /gene="pyrR"
                     /locus_tag="IWO89_000943"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015694870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional pyr operon transcriptional
                     regulator/uracil phosphoribosyltransferase PyrR"
                     /protein_id="HAQ0455715.1"
                     /translation="MQAKEVVDQVTMKRALTRITYEIIERNHSIQDIVLVGIKTRGIY
                     IASRIAERLKQLEDIDIPVGELDITLYRDDKKENPEEPELHSSDIPVSLEGKEVILID
                     DVLYTGRTIRAAMDAVMDFGRPRKISLAVLVDRGHRELPIRADYVGKNIPTSRAEEIL
                     VEMQELDGQDRIMILKEED"
     gene            36101..37384
                     /locus_tag="IWO89_000944"
     CDS             36101..37384
                     /locus_tag="IWO89_000944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil permease"
                     /protein_id="HAQ0455716.1"
                     /translation="MSEKKGFHNDDVVLDIHDRPTPGHWVGLSLQHLFTMFGATVLVP
                     ILVGIDPGIALVSSGLGTLVYLFTTKGKIPAYLGSSFAFIAAMQLLMKTDGYPAIAQG
                     AVTTGLVYLIVALIIKKIGSAWLDKILPPIVVGPVIMVIGLGLAANAATNAMYNQGTY
                     DFKYIAVALITLGLTIFFNMWLRGFLGLIPILLGIISGYIVALLFGIVDTKPIVDAAW
                     FAVPNFEIPFVQYEPKLYLGAITTMAPIAFVTMTEHIGHLMVLNKLTKRNFFEEPGLH
                     KTLTGDGLAQIVAAFVGGPPVTSYGENIGVLAITRVHSVFVIGGAAVFAVILGFVGKL
                     SALILSIPGPVISGISFILFGVIAASGMKILIENKIDFDKKKNLLIASVILVVGIGGL
                     VFEVGTFTLSAMALATVLGIILNLILPETSRSEEN"
     gene            37397..38323
                     /locus_tag="IWO89_000945"
     CDS             37397..38323
                     /locus_tag="IWO89_000945"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase catalytic
                     subunit"
                     /protein_id="HAQ0455717.1"
                     /translation="MIIKSERISLKHLLTVEALTDQEVMGLIRRGQEFKQGANWTPQK
                     RQYFATNLFFENSTRTHKSFDVAEKKLGLEVIEFEASTSSVQKGETLYDTVLTMSALG
                     VDVAVIRHGDENYYDELIQSKTIQCSIINGGDGSGQHPTQCLLDLMTIYEEFGHFEGL
                     KVAIVGDITHSRVAKSNMQMLKRLGAQVFFSGPREWYDDEFEVYGHYMPLDELLDQVD
                     VMMMLRVQHERHDGKESFSKEGYHQEHGLTVERAKKLQKHAIIMHPAPVNRDVELADS
                     LVEGLQSRIVQQMSNGVFVRMAILEAVLNGKA"
     gene            38342..>39096
                     /locus_tag="IWO89_000946"
     CDS             38342..>39096
                     /locus_tag="IWO89_000946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HAQ0455718.1"
                     /translation="MKTLIKNGQINTRKNMTTPAEIWIEDGRIKAIGTGFSEAEFDEV
                     FDAKGQLITPGLVDVHVHLREPGFTYKETIEAGTRSAARGGFTTVCAMPNLNPVPDTA
                     EKLRQVYDIIRKDAVVKVLQYAPITENLRSEKLVDQEALIEEGAFAFTNDGVGVQTAG
                     TMYLAMKEAAKNNKALVAHTEDESLLFGGVMHAGKKAEELGLPGILSVTESSQIARDL
                     LLAEATGVHYHVCHVSTKESVRVIRDAKKAGIHV"
BASE COUNT        12924 a         6751 c         8280 g        11141 t
ORIGIN      
        1 attaccatat atggtatatc tttgtctttt ttttgctagt caaagacgaa tcactcgcca
       61 taaatgaaca gattgtatta tgataagtaa gaagagcaaa gacaaggagg aattgaagat
      121 gacagctatc tataactcag tcaccgaatt gattggcaaa acgccaatcg tcaaattaaa
      181 taaaatcgta ccagaagatt cagcagatgt ttttgtaaag cttgagtttt ttaatccagg
      241 aggaagtgta aaagatcgta ttgctttaag tatgatagaa aaagcagaac acgatggtct
      301 actgaaacca ggtgatacta tcattgaacc tacttctgga aatacaggaa tcggattgtc
      361 gatggtagga gttgcaaaag gatataaagt aatcatcgtg atgcctgaga cgatgagtat
      421 cgaaagacgt ttattgatga aagggtacgg cgcagagttg attttgactc ctggtgcgga
      481 tggtatttct ggatcaatca gagaagcaga acgtttggca aaagaaaacg gctacttttt
      541 accattacaa tttgaaaatg aagcaaatcc tcttgtgcat gagaagacga ctggtcctga
      601 gatacatcaa gcatttggag taaatggctt ggacgcgttt gttgcaggta tcggtacagg
      661 aggaacgatc actggcgctg gccgagaatt aaaacgtgtg tatccgaaaa tcgaattaat
      721 cggggtagaa ccagcagaat ctgctatttt agaagggaaa gaagcgggac ctcacaaaat
      781 ccaaggaatc ggtacaggtt ttgttcctaa aacactggat acttctgtgt atgataaagt
      841 cctttctatt agtggagacg aagcgatgga aacagctaga gaagttgggc gaaaagaagg
      901 gattttagta ggcatctcat ctggcgctgc aattgctgcc gcgctaaaag tcgcaaaaga
      961 attaggaaaa ggcaagaagg tattagctgt ggttcccgac aatggtgaac gttatttatc
     1021 tacagccctt taccaagaga tataaggcac ttggtttttc tccagatagg tttatgtaaa
     1081 aaacttttaa agatgaaaag aacacacaaa aatttatgat gagaaaaatt atttgaatag
     1141 agacgtagaa tagtgaacgc gactgtttta cgtctttttt cattaaaata caataaaatt
     1201 gaattttctt ttcatgttaa gtggatttta aaatgtaaaa atagtatgat aagacgagaa
     1261 taacaacgtg gggtgtttac atggaaaata agttagtcga agaggttatc gaaaagttaa
     1321 aaaaagagaa cattcgaatc acacctcaac ggtacgcggt tttagaatat ttgattgaac
     1381 atcattccca tccaacggct gatgagattt ataaagcact tgaacatcgg tttccaaata
     1441 tgagtgttgc gactgtttac aataaccttc gcttatttac ttcgattgga tttgttcaag
     1501 aaatgaagta cggcgatgct tctagccgat ttgatttcag ttcaaagcgt cattatcatg
     1561 taatttgtga aaactgcggt aagattgttg attttcatta cccagggctt gaagatatag
     1621 aaatggctgc aagcagactg actgattttg agattaatga acatcggttg gaattgtatg
     1681 gactctgtcc ggattgtaaa gagtcagcaa aaaaggcgca aatgtaaaaa cagtttctac
     1741 aagtgttata cctaaaaaaa gctagctgtg taaaaaaaac gctagctttt ttattttttt
     1801 gtcttttatt cacaatctta gatataaaag tgaatattat taaaataaga atcttgtaga
     1861 tattgggttc ttatgcttga aaaggaatga aactacaaac aaacctttgt gaattcacaa
     1921 gcattaaact gttgtatccg ttttatttct gttatagtag ttttgtcgaa ggacaaaatg
     1981 ttaatcggag ggaccataaa tgaaagtagt agtaattggc tgtactcatg caggtactgc
     2041 agctgtaaaa agtattttaa aaaatcatcc agaagcagaa gtgacagtat atgaaagaaa
     2101 tgacaatatt tctttcttat cttgcggtat cgcactttat gtcggcggag tagtaaagga
     2161 cccagcaggc ttgttctact caaatcctga agaattatct tcattaggag caacagttaa
     2221 aatggaacac gatgtaacag acgtcgatac agaaaataaa acagtaacgg caaaagatct
     2281 gaaaacagga gaaacagaaa cggtttctta tgataaatta gtgatgacaa ctggttcatg
     2341 gcctattatc ccaccgatta gtggaatcga tgcgaaaaat gtactcctat gcaaaaacta
     2401 caatcaagca aatgaaatta ttgcacaagc gaaagaagca aaacgtgtag taattgttgg
     2461 tggtggatat atcggtattg aattagtaga agcttttgtc gaatctggaa aacaagtgac
     2521 attgattgat ggattagatc gcatcttgaa taaatatcta gataaaccat tcacagatat
     2581 cttagaaaaa gaattgacag accgcggtgt tactcttgcg ctaggcgaaa atgttactga
     2641 atttatcaca gatgaagaag gaaaagtgaa acaagtagct acaccaacag atacatttga
     2701 tacagatatg gtcattttat gtgtgggctt ccgtccaaat acaaaactga tcgaaggaaa
     2761 agtagataca ctgccaaacg gggcaatcaa agtgaatgaa tacatgcaga caagtaatcc
     2821 agacattttt gcagctggag actctgcagt agtcaactac aatccaagtg gcacgcaaaa
     2881 ctatattcct ttggcaacaa atgccgttcg tcaaggtttg cttgtgggaa ataacttgac
     2941 agatcataaa atggcgtatc gtggtactca aggaacttca ggactttacc tatttggctg
     3001 gacgatcggt tcgacaggtg tgaccaaaga aagcgcaact ttgaacgatc tagaagtcca
     3061 agcgactgta tttgaagata attatcgtcc agagttcatg ccaactactg aaaaagtaat
     3121 gatggagcta gtctatgaaa aaggaacgaa tcgtatcgta ggtgctcagt tcatgtccaa
     3181 atacgacatt acacaatcag caaataccat gtctttagct gttcaaaata aaatgactgt
     3241 agaagatctc gcactttcag atttcttctt ccaaccacat tttgaccgcc catggaatta
     3301 cttgaaccta cttgcacaag cagcgctagg cgaaatcgaa actgcacaat aaagaaaaag
     3361 ggttgtgaca caattttgtc acgaccctct gtcttttttt gtgaaaagta caattagttg
     3421 tctttcttcg aattttttgt cacaataagc agcaaagata aagctgttac ataaaagaac
     3481 aagcagcttt atttgatttt gaaaagaaag gtgagaaaga agatgaataa agaatatttt
     3541 gtccaacaag atgcattttt agaagattat aaaagtgctt cgaagaaatt aacgatcaat
     3601 attgatgagt tggcagatca agtactctct cagttgaatg aaacgcaaga ggaatattac
     3661 acgatgccag caaatgacgc caaagataat gtttctcatc gttttccttt cagtagacgc
     3721 atctactcag atgaaaatga cggcacattg aatacagagt acacatatga aggtcagcca
     3781 tatgtgctag aaactgatga tgatctttaa agacagtttt tgaaaaggcc gctcatcctt
     3841 aagtgatgac aaaaaatcat cacaattacc gatgactgtc tctttactat cttttaatca
     3901 gaaaaagggg ttatgacaag ctttgtcaac cccttttttt cttggtttca gattgggttt
     3961 tcctacaaaa tatttttatc tttgttgaat aggaaacaaa aaaacaatat aatgaaagaa
     4021 aaagaaggtg tagaaatgga taaaagacaa ctgctaatag aagaatatct ggatttagct
     4081 aatgagatta ttcatcgtaa taaaccagaa atggaaaaag aatttgcagg atttactcta
     4141 aatgaactag aagtgattga acatatcggg aaaatccctg atcctaacgt gacaaaatta
     4201 gcgaatgcta gttacatgac tagaggagcc atcagtaagc taacaaaaaa actgatagct
     4261 agagggatca taaacactta tcaaagcgaa gagaataaaa aagaaatcta ttttcaattg
     4321 acgccaatcg gacaaaaaat aaggaataga cacgagcaac tccatcaaaa atgggtacag
     4381 cgagatgcta ctgtatttga aaatatgtca aaagaagaat ttgacacagt atttcgtttt
     4441 atcggtcgat acagagaatt tatacgaagt agcaaatgaa gctactattt tttattgtag
     4501 tgataatagg caaaaatcat aagcaaaaat ccgaataccg tcggaagaaa tggtgctgca
     4561 gcataaagct gtcctgcgat tagcgggccg atgacgcgcg atagagattg tatactttgg
     4621 gtaccgccca ttacaagacc ttgctggtta tctgagacag aattggatag ttggccattg
     4681 aatacaggag taaaaataga atcaccgaaa ccataacaga taatcccaaa taaaaacaaa
     4741 atcggcagta gaagaattcc agataatgtg atgaatagat aggcgaggat ttcactgaac
     4801 atccctattc ttgtgatctg ctgttctgaa aattttttga gcaatcgagg cataatgaac
     4861 aattgcgaga cagtatccaa gattccaatc aaagagaagc ttaaaccaac caacccagcc
     4921 ttccactgaa aggcgtcaat cgaaaattga gaaaagatag attgaagaga gccagctgcc
     4981 aaccaaacag caaaacctgc tgtcaataaa ggaataacgg atgaaatctt gaataattgt
     5041 tgtaattggt gaaaaggtct tacatgggag aaacttaaat ctactgaacg tttttttatt
     5101 ggaaggcttt ctagcataaa tgtgtagcca taaacggcat ttaagaaagt aaagagtgct
     5161 ccaataaata taggaacaga attccccaac tcagctaata gaccccctat aataggaccc
     5221 agcgttgttc ctataccgac taaagcaccc atccatccaa aaactttggt tctttcatta
     5281 ggttccgtga tgtcggcaaa gtaagcatat aaggtggaga tttctcctgc cgtcaatcct
     5341 tcaatgatac gaccaaggaa caacatccat atcgaatttc ctaaaccaaa aagcaaatat
     5401 ccgatacttg atcctaataa actgataata aggatcggat gacgcccaaa atgatcactt
     5461 aagctaccga gaatgggggc agataagaag gcagctagag cataaacaga catgagcaaa
     5521 gtgatataga aagcttgctg atgtatggag tgagaataag gcagcgttaa aaacggaagt
     5581 acaggactga cgatagtaaa tcctaatcca gtgaataaga cagagacgta tccaaaaact
     5641 ttagaatgtt tatttgtgaa actgtacatg taaaaactcc tttatgcaat aatgttaact
     5701 tggaaacaaa aatagtgtat agtaattttg tttccttgtc aacattctaa ggaacagcga
     5761 aatctactta atttatttat tccgaaaagt gaagatacgc atcaaaaaca tgaacaaaca
     5821 aaaatcaagt agacgacatt gtaaatctga ccgatcagtg aagtaaatag aaaagaaacc
     5881 tgttcttgct tcccatgttt tttttacgta tactgtgatt gagcggagga gaaatcatgt
     5941 tgaatttaga aaattactta agcgctggac aaacagtcat atctaatctt ttacttaaaa
     6001 attatcatag gatcggtcta caatctgaag aatttttatt tgttctacag ttgcatatgg
     6061 cacagcttga aggagatact ttccctgatt tgcagatgat tgctatggat atgggaatca
     6121 aacaagatca gatttttcaa atactggatc gtctcgtaac aaatggattt atcaagatag
     6181 aaacgacgat tgtcaacggt aaaaaagcag atcgatataa tctttacccc atctatgatc
     6241 tattaggtga gtatttaaaa acacaagaaa aaaaatacga agaaaaaaca caagaaaaag
     6301 aaatccagtc agtgtatcaa ttatttgaac aagaatttgg acgcccgctt tcttcgatcg
     6361 agttccagcg gatcggacaa tggctagaag aagaccatta tcagccggaa attttgaagc
     6421 tagcactgcg ggaagccgta ttaaatcagg cttatagttt taattacgtt gaccgcattt
     6481 tgctatcgtg ggaaagaaaa aatctacgta caaaacaaca agtggaagaa gaccaaaaac
     6541 gaagaaaaca gcagcttctt caaagagaag tagaacagtc agctcaacaa gaagaattgc
     6601 cgaaagtctc actgaaaaat tggttggagg agtagcggaa tatgttaagc aaacaaaaaa
     6661 caatggaagc tctagaaacg atgtatggaa tgtttcccga agcacatgga gaactcaagc
     6721 acaataatcc atttgagtta ttgattgcag tgatcttaag tgcccaggcg acagatgtat
     6781 cagtgaataa agcaacgcca gatttgtttg cctcttttcc gacacccgat gcgttagcag
     6841 aagcttcaat cgatgaaatc attcttaaaa taaaaacgat tggtttgtat cgaaataaag
     6901 caaaaaacat caaagcgtgt gcacagcagc tgattgaacg tttcgatggg caagtgccaa
     6961 cgtcaagaga agaattgatg tctcttcctg gtgtaggcag aaagacagca aatgtcgtat
     7021 tgggggatgc attcgggata ccagcaatcg cagtagatac ccacgtggaa cgtgtctcaa
     7081 aacgcctgag gatctgcaaa ctagatgcta cggtcatgga agtagaagaa acactgatgc
     7141 ggaaagtacc acaagaatta tgggtaaaga cacatcatac actgattttt tttggtagat
     7201 atcattgcac ggcaagaaat cccaagtgcg aggtatgtcc tttgctatca atatgtcaag
     7261 atgggaaaaa tcggatgcgt ctaaaagaaa aggcgctaaa aaagaaacca cgcctgtaga
     7321 cgtggtttct ttaattgcaa ataaatgtat gaattatctt gttattatgg agaaaagctg
     7381 tttcgtttat cttttttgca tgaaaaagta aatgtacata ctcagtagct aggatggtaa
     7441 cttattacaa tttattgcga taaaaataaa agttgttatt tttattggga aaatacccga
     7501 tgtagaaaat aatgccttcc aatataccta taaaataagt ccagtattcc aaaggattat
     7561 ataaataagt gacgtatcta tctaaaggtg taataaaaat gataagtgat aataaaaaaa
     7621 taccatttat aactaaacat aaactaatgg atttgggcgg tttcaggctc atcagaaaaa
     7681 tatagattct ggtgaacaac aaccataaaa aagtgagaat cattgcatga gagcttcttt
     7741 ccataaattt tcctttaaga gtagtgctta aaagtacaga aaaaataata aatgcaagga
     7801 taggagcaat taaagtaaat ttatttaata ataaacaaaa tttttcagct tttttattct
     7861 tggaataacg aggtattgtt ttcgataaaa aaagatggtg cagacaaatt atgataatta
     7921 agcatactat cagataactc aaaatgaagc cttctttcaa aaaattattc aaaaccatat
     7981 attgtatttt ttatttgata ataacaatgg gattattaag aagtggcact aaataaaaga
     8041 ctagtgtgta tttagccgat ttaattataa acaagtaaat ggataaagta tatgtaatga
     8101 taaaagaaaa atggattttc taatttttaa gctaaatgtt agatcacaca tgtatatgct
     8161 ggatagtctt ataaaaaatc atgtaataca gcttggtaca tgatttttat ttgtagctgt
     8221 tttttacata gacacaaaaa agaaaccacg cttgtagacg tggtttcttt tttactatct
     8281 gtttccagaa gctgaggtgc tgctggatgg tgtatggtca tttgcagcat ccgctccgct
     8341 agttgctggc tcactggaag aagcaggagg agcagatgaa gacggtgtcg agctttcttc
     8401 ggatgaagaa gaaggaaccg ttgttgaaga cgctggctcg cttgtttctg ctgtcgtgct
     8461 ttcttttgag gactcagcag ttgattcgct ttgactagta gagctagagc tttgagtcgt
     8521 tgtctctgtt gttgagcttc caggagtctg gacatagctg gaagatggaa tagttgttga
     8581 tggtgtaatt gcattcgatc tagctgtata ttgatctttg tagtacagtt caccgcccac
     8641 acgaattaat ccgcttggca tctcccagtc tgtatttgtc acgttagctg aaacatactg
     8701 cataagctcg cgataaacat cagaggctac gtgtgacgat tctgaagtga caggtgtcat
     8761 ttttttattg taacctgtcc acacggaaat cgaataattt ggcgtatagc ccgcaaatag
     8821 gatatctgga tacaccccag aagaaatacc aagttttgca tactcgtcat ctgtgtaatt
     8881 ggatgttccg gtcttaccgg cttgatataa cccagcgatt tgcgcattcg taccagttcc
     8941 ttcagtgatc gtatctttta aaatgtctgt gatcatataa gctgtttctg gagacatggc
     9001 tgtttttcca tctggctcat aagtttcttc tgttccgtct tggaagacga ttttattaac
     9061 atattgaggt ttgtaataag tccctccatt ggcgaaagcc gcataggctg ctgccatttt
     9121 taaggaagaa gctccgtatt ttgtcccatc ttgttcttcc gtattactag agatcgcatt
     9181 cgattgatgg atcgtactgt attcgatccc aagatttttt aagaaagaag ccactttatc
     9241 tgaaccgact tcattgaata atttgactgc tggaacatta cgggataagt aaagtgcttg
     9301 gcgtaaagta atggtgccca tatattgatt atcccagttt cctacaggag tcgaagtccc
     9361 ttcatagtta tacggtgcgt cagtaatagt ttttccagta gaatatttca aatattcaaa
     9421 agctggtccg taatctgtga caggtttcat cgtagaacca aaatcacggg aagtattcac
     9481 tgctaagttg ttgcctaaag ttacgtcttc tgcgatgtga cgtccgccaa tctgggcttt
     9541 tactttcccg gtattggtgt cgataagggt ggaggcaact tgcatctcgt cgtctgggta
     9601 agagacatac tgatccgtgt tgacgatatc atatagtttc ttttgggcgt ccagatccaa
     9661 attcgtataa atctctaaac cgtctgtgta gacattctta tctgtttttt cttgtacttc
     9721 attgatgact tctttgacat aattatcgac gatcttagta ttgtcatcgg attgagttag
     9781 ttcttgcaga ccatccgtta ccggtacatt aacagcttga tcatattctg tttgagagat
     9841 tttttcgttt tgaagcatcg tatacaaaac agtatctcgt cttttcttcg cctgatctgg
     9901 ataaacataa ggatcatagg cggaaggggc ttgaggcatc ccagcaagca atgctgtttg
     9961 cggtaaagaa agttctgata atttcttacc aaagtacatc tcagaagctg tctccatccc
    10021 gtacaagccg tttgacatat acactttatt tacgtagtag gtcaagatct cttgtttcga
    10081 ttttttttgt tccaatctta cagccatcca cgcttcttgg gccttgcgtt tcagagtctg
    10141 atcctcggca ctggtggaga agaaggaaag tttgatcaat tgctgtgtca aagtacttcc
    10201 gccttgcagt ccgccagagg taaagttaga gagggcagaa ccgatgatcc gaatagggtc
    10261 aaccccgatg tgtttataaa aacggcgatc ttctaccgac acgatcgcat cttctaatgt
    10321 tttcggtaat tcattcgcag aaattttttc acgcttttct gcgcctaaat cttcgaatag
    10381 ttcgccatct tgtgtatata gtttggaaga tacggtagcg tctaattttt tatccgtcaa
    10441 ttcgggcgca tcttttgcgt aataccagaa aagtcctaca cctgctaaaa agaggataca
    10501 gaaaaatgag agtattccta aaaggatctt gataaataat ccttttttat gtgttccaga
    10561 ggactttcca gaacctttgc cagaattctt cttcacactc tttttaggag taggctgttt
    10621 ctgtcgtcta gagcttctag tttgttcatt tgccattctt gctttctcct tcaatagata
    10681 atgttgataa atagaagtca accgcgtcca aataaggaat ccgcggagaa ataccagttt
    10741 tgatctctat tcctagttct tcgatcaaag ataaaggtag tgatttgcgt cctttttcct
    10801 tttgggcgtc ccagtaagca atcaaatgaa cactattcaa ataaaaacag cgcttcaaag
    10861 acgaaaacca taacaagaca aagcagaccc cattttgttc gaggcatgct ttcatatgtt
    10921 cgatttgatg ggcatggaag tttttaaagg gaaaagatgt tttgttcttg gtttcttttg
    10981 cttcaaagtc caagtaaaag cctcgataga cgccattata gtctgtagtt gaggcttggg
    11041 taaagtaggc ttccttgatc acagcagcac ttctcttagg atagtctact ttcacgattt
    11101 ggataggggt aggcttttta tggataaccg ccaacccgcg ggaaagataa tatttattac
    11161 tttcattgat tgcttcttca aagcgcatcc cccgattgcc gaattcagtg atctgatttc
    11221 ttttttcttg aattttttcg acgggagagg cattcttata ataaggttgg ccatttggat
    11281 agcgcaatac catctttaat cacactccta ggccacatta tagcaaaaaa gcggatagaa
    11341 agaaaaggaa tctgagatgg ataagtcaaa agtaatctat gtgtctgggt atcgaagttt
    11401 cgaactcggt gtttttaaag aaaatgatcc taaaatccaa gttgttaaaa atgttttaaa
    11461 aaaagaactc catcagttag cagaagaagg gctggaatgg gtacttgtca gtgggaattt
    11521 gggcgtcgaa ctatgggctg tgcaaatagt agcggaattg aaagaagaat atccggaact
    11581 atctcttggt attttgtatc ctttttctga ttttggtaat aactggaatg aagggaacca
    11641 agaaaaaaaa caattagcag aacaattagc agattatgtc gaagctgtta gccatcaacc
    11701 ctaccaatca cctagtcagc taaagaatca cactcgtttt atacttgagc atacagtggg
    11761 ctgtctgctg atctatgatg aagaattccc tggaaaaacg aagtattttt ttgaagatgc
    11821 acagaattac caagcagatc atccctacga aattcggctg atcagtatgg acgatcttca
    11881 aaattttagt gaataatcga tattgtgttt gatttatcaa cttttttcct atacaatgaa
    11941 aaaggtagaa atgttattac gtaaatgaaa actagaggtg taaatatggc gaatttggtt
    12001 tatagtccta aagacatttt acaacaagaa ttcaaaacaa aaatgcgcgg ctatgatccc
    12061 gttgaagttg atgaattctt agacaatatc atcaaagatt atgaagctta cagcaaagaa
    12121 ctccttgctt tacaagaaga aaacgatcga ttgagtgcaa aagtagctca attatctaaa
    12181 actcaaggag cagctcaaac acgcgtgcaa caaacagagg caccaaagag cgcagcagtg
    12241 accaattttg atatcttaaa acgcttgtca aatcttgaac gtgaagtatt tggtaaaaag
    12301 cttgatcaac aagcaagtgc agtcaaacca gcacaaccta atccaaataa ctacacaaat
    12361 gcagacacaa gcttagatga taatgaaaaa acacgtcaat tttaattttt gattaatgaa
    12421 tttgcggttt tcgggtaatc gcggtctgac ttgatcaggc tgaggaaagt ccatgctcgc
    12481 acaggctgtg atgcctgtag tgttcgtgct tagcgaaacc ataagctaag gtactctttg
    12541 gagtaacggc aggaaaacaa gctaaggctt cggctatgct taagtatcct tgaaagtgcc
    12601 acagtgacga agcggctggg gaaacccatc cggtggaacg cggtaaaccc ctcgagcgag
    12661 caacccaaac aatggtaggg gcgctttttg tctggaagaa gaacaggaca aaaaggcagt
    12721 ataactgcag atagatggtt accgctttgt tttttctccc tggaaaagca aagtacagaa
    12781 catggcttat agaaaaccgc aaacatcagg gaattaggtc gtgacaaaag taatgcgtta
    12841 taagaaacaa gaaaaacatt tgaaagctat ttttcaatac ttcagatgaa tcgttcttat
    12901 tacaaaacaa acttttgtat caccggttat tcggaatttg agtaaaagtg tggggtagga
    12961 ctgatgtccc acctcctttt tttataaata aggagattgt gtatggaaaa aaacaggaag
    13021 ttcaacctcg tcgcaactgc ggcaagcggg ctagaagcac tggttggtaa agaattaaga
    13081 gatcttggaa tcgaatgcca agttgagaac ggacgtgcac gttttgaagg aacaatggaa
    13141 acgatcgcta cagctaattt atggttacgt acagcagatc gagtaaaaat cgtggttggg
    13201 gaattcgatg cgtatacgtt tgatgaattg tttgaaaacg taaaagcatt gccttgggaa
    13261 gattttcttc cactagatgc agcttttcct gtagctggaa aatcgatcaa gtcgaaacta
    13321 tatagcacac ctgattgcca agcgatcaca aaaaaagcaa tcgttgaacg tctccgtacc
    13381 tattatcacc gtccagcttc tgtaccactg acggaaactg gcgcacattt tcagttggaa
    13441 gtagctttgc tgaaagacca tgtgatggta actctggata cgacaggacc aagtttattc
    13501 aaacgtggtt atcgcttaga aaaaggtgga gcaccattga aagaaaacat ggcagcagca
    13561 cttattatgt tgacaaattg gcgaaaagac cgtccatttt atgatcctgt atgtggttca
    13621 ggtacgcttt gtattgaagc agccttaata ggccataata tcgcaccagg attcaatcgt
    13681 gagtttgctt gcgaatcttg ggattggttc agtcaagaaa tcatggaaaa tgttcgatca
    13741 gcagccgaag aaaaagcaga ttatgatatt gaattagata ttactggttc agatatcaat
    13801 ggacggatga ttgaaattgc caaagccaat gcagaagaaa tcggattagg ctcttcgatt
    13861 acctttaagc aacaagcagt caaagacttc aaaacggata aagaatacgg ggtcatcgtg
    13921 gctaatccgc cttacggaga acgtctagga gaagaagaaa ctgttcggaa attatataaa
    13981 gaaatgggcg atgtattccg tccattaaaa acatggagta aatatatctt gactagcgac
    14041 ttagcgtttg aggaatttta cggacaaaaa gcaaccaaaa aaagaaaatt gtacaatggt
    14101 gcattgcgta cggatctatt ccagtattgg ggaactcgtc cgcctagaaa aaaagagaaa
    14161 gacgaataat cttactaatt gattcattca attggatgtt tggaggaata gggaatgaaa
    14221 gaaaaagagt ttttacaaga agtaaaagaa atcaatcttc tacaacaagc tttaggaatc
    14281 ctcgattggg atacacaaac aggtatgcct gaaaaagcta gtgcataccg aagtgaagta
    14341 gacagctatc tttacagcat ttatttttca aaaaaaatcg gtccaaaaat caaagaagcc
    14401 attcgctatt tttctgaaca tccggaagaa ttgtcggaag ttggaactgc cgtttttgcc
    14461 aaagtaaaag aagagtatga tttggaacat cgcgtaccgg aagatcttat gagaaaatat
    14521 acagctgccg tttcttcagc acacggacaa tggcaaaaag ctagagaaac acaacaattt
    14581 tctgattttc aacacgcatt gaatcaaaat attgaattaa caaaacagtt gataccttac
    14641 tggagaaaag atgaaaagac gaattatgat gttttgctga atcaatacga acccgggatg
    14701 actgtcgaaa tccttgataa ggtatttgat cagcttaaag aaggaattct ttcgatccgc
    14761 cgtacgctcc aagaaaaagg gactgaacct gaaacagact ttttaaatcg gagaatgacg
    14821 aaaaaacaac agaagagatt tgtagtgaaa gtaatcgaac aacttggtta tgattttagt
    14881 cgtggtcgtt tagatgacac tgtccaccca tttatgactg gaattaatcc gaatgatgca
    14941 cgaatcacga cacgatggaa cgaaacagat ttcaaaatgg ctgtttttgg agtgatccac
    15001 gaggctggtc atggtatgta tgaacaagac atcgatgata aatatgcgta tacaccggtt
    15061 tatgaaggta catcaatggg tatccatgaa tcacaatcgt tgtttaacga gatcattatt
    15121 ggaagtagtc gcagcttctg gaaaaagcaa tatcctttct ttcaagaatg tgcagaagga
    15181 acattcgatg atattccttt tgacaaattt tatcgttcat tgaaccatac aaaatcaagt
    15241 ttggttcgta tcgaggcaga cagtttgact taccctctcc atatcatcat tcgttatgaa
    15301 atcgaaaaaa tgattttcaa tgaaggggta gatgttgctg aactacctga aatatggaat
    15361 caaaaatatg aagagtatct gggaatacga ccaacaaatg atgtggaagg catcctacaa
    15421 gacgttcatt ggtcaggtgc aagctttggt tattttccct cttatgcatt agggtacatg
    15481 tatgcggcac aattacttca ttctttgcag caagaaatag atgtggaaaa tgttctagcg
    15541 tcagacgatt attctccaat caaaaactgg atgtcagagc atatccatca atatggtgca
    15601 tctagaaaac caaatcaatt aatcatggat gcaacacatg aaccgcttaa tccgcaatac
    15661 ctgatcgact atatgaaaga cctgtatttt aatgtatatc aggtaaaata aaatacaatt
    15721 tacaaagata gctgaaattc atctaatcta tagatgtttt cagctattta cttaagaaag
    15781 aagtggaacg aatggataaa ttacaagtga tcgctgatta tagtatggac caattagcgc
    15841 atgatcaaac aggacatggg agcgatcata ccaaaagagt tgtcaagtta gctgaaaaaa
    15901 ttttggagac ggaaccgcag gcagaccgct ttgtgacgct tgcagcagct tacctacatg
    15961 atacgattga tgataaagtc gttaaaaatg agaacgaggc aaagcaacag cttcgtgtat
    16021 tccttcgctc tttgccaata gtagaggaac agatctcgat gattttttcc attatcgaaa
    16081 atatgtcctt ttctaaaaat ctctctgaag cggccgaact atctctggaa ggtaagatcg
    16141 ttcaagacgc tgatcgtatc gaagcattag gagcaatcgg cattttgcgt actgcttatt
    16201 tcggcggtgg acatgggcac ccgatttttg atcctgaact gtatccgcaa acattcaagg
    16261 acaaagaaaa ttaccgaaaa ggcacgacag tgatcaatca tttttatgaa aaactgttct
    16321 tgctgtctga taaaatgaat actgattacg gatatgaaga agccaaacga agagaaacct
    16381 tcatgcgtga attcttagaa gaattttttc tggaatggtc tattgacgat gaaaatttta
    16441 ctccagacaa ttggatcaaa aataacaaaa aatgatggag gttgtgacag aagtggtcag
    16501 ctccaagata tttcggatca ttttccgtaa aagttacttc tggaacactg tttaccaaga
    16561 ggtcgtgagc ctgccgttca gctccgagtc acaaggaaca atttcaaaaa atagcttctc
    16621 gtgtttgatg aatattgtaa cttgtgattc ggaattggca ggagaacacc gtttattcgg
    16681 aagaatagga ctgtgacaag acttttgtca cagtcctttt ttcgaattca acaagtaaaa
    16741 cttttaagaa atattcagag agtaaaatta aaaattgctg ataaacgaag atggaaattt
    16801 tatgtatttt tattggtcta tgcaataaat aaattaaata ttcagaatac aattgatttt
    16861 aacgaaaggg cttctatcaa tggttgtgtt gtatttatct gcccaggtag agtaaaatag
    16921 atcattatct aaaaggtaat tgttcatact atataagtca tttgaaagta aaaagatact
    16981 gtaaataaaa aatgtggtta aataattctc ataatcagtg taagcgttat caaaaatata
    17041 gcatacttgc tttgtagatg aatgacacga tatatgtttg gtatgttctg caagacagat
    17101 gaatatggag gtggagacaa tgtcttccaa agaactaaca atgtatttag agaaagaatt
    17161 ggatcaactg aaagaaaagg ggttatacaa tacgatcgac atactagaaa gtgaaaatgg
    17221 tgcttgtatc atcgtagacg gaaaaaagat gatcaatcta gcatccaaca attatcttgg
    17281 atttgccaat cgcgaagaat tgaaaaaagc atgtattgaa gcaaccgaaa cttatggtgt
    17341 aggagcagga gcagtccgaa cgatcaatgg ttcgctgaaa atccatcagc aattagaaga
    17401 aaaaatcgca gaattcaaag ggacagaagc agcaatcgcc tttcaatctg gattcaactg
    17461 caatatgggc gctatctctg cagtaatgac aaaagaagat gctatcttat cggatgaatt
    17521 aaaccatgca tctattattg acggttgccg cttatccgga gcaaaaatca tccgaattaa
    17581 acatcaagat atgaaagacc ttgagaaaaa agcaaaggaa gcgatagaaa gtaagaaata
    17641 taaaaaaatc atgtatatca cagatggtgt tttctccatg gatggagaca ttgcacgatt
    17701 gccggaaatt attccgattg ttgaaaaata tggactgatc acctacgtcg atgatgcgca
    17761 cggctcaggc gtaactggaa aaggtgcagg aaccgtcaag cattttggat taagcgacaa
    17821 aatcgatatg caaatgggca cgttatcgaa agctataggt gttgttgggg gttatgtagc
    17881 tggatcgaaa acactaatcg actggttgaa agctagaagt cggccattcc tattttctac
    17941 ttcgctaact ccaggtgcag ctgcagctgc attggcttcg attactttga tgcaagaaca
    18001 tccggaactt gtcgaaaaag tttgggaaaa tgctaattat tttaaagaag aattgaaaaa
    18061 agtaggctat aacatcggta tgtctgaaac acctattact ccagtcattt tgggagacga
    18121 aaaagtgact cagactttct ctaagaagct gatcgaacat gggatttatg ctaaaccaat
    18181 cgtttatcca actgtgcctc ttgggacagg caggatcaga aatatgccga ctgcggaaca
    18241 tacgagggaa atgcttgatg aagcagtagc agtttatcaa aaaataggaa aagaaatgga
    18301 aattatttaa ctaaatttag ttttttcgtc tatttaaaat tagagaacat gttcacgcat
    18361 atattgttaa tcatctgaag atagtagtaa aaaagatcag ggaggatcat catgaaaaaa
    18421 gtattggtga caggatgttt aggacaaatt ggttcagagt tgatcacacg gctacgtcaa
    18481 gaactaggca gtgaaaatgt cattgcgaca gatatacgca tgcctgaagt cattgagaaa
    18541 aacgatatct ttgagatcct agatgtaatg gattacgaaa aaatgcgcag catagcagaa
    18601 caatatcaag tggacacatt gattcatttg gctgctcttt tgtcagctgt tgcagaagca
    18661 aaaccacaat tagcctggaa tttgaacgtg actgggctgg tcaatgcatt ggaagtcgct
    18721 agagaattcc agctgaaatt ttttacgcca agttcaatcg gcgcgtttgg tccaaatact
    18781 ccaaaagatc ataccccaca agatactctt cagcgtccta caaccatgta tggggtaacg
    18841 aaggtagctg gagaactact atgtgattat tattatgtga aatatggcgt agatactcgt
    18901 ggtgtccgtt tccctggatt gatttcttat gcaaccttgc caggaggcgg tacgacagac
    18961 tacgcagtcg atatttatta ttcagcaatc aaagaaggaa agtacactag tttcattgat
    19021 caaggtacat caatggatat gatgtatatg ccagatgcga tcgatgccat tataaaactc
    19081 atgaatgcag atcctgatcg attggttcat cgaaatgcct tcaatatatc ggcgatgtct
    19141 tttgacccgg aagagatcaa agcatctatt caaaaaatca taccagattt tgaaatggat
    19201 tatgatgtgg atcctgtacg tcagtcaata gcagaatctt ggccaaattc actagatgtt
    19261 tcttgtgcgc aagaggaatg gggcttttcg ccgaaatacg acttagatgt gatgacaaag
    19321 gatatgttgg aaaaactgaa agaaaaattg gtagagacgg tataaaacaa gaaccacatc
    19381 caaaaagttt tttatacttt tgggattggc tatggtatga aaggggacaa gggatggaag
    19441 aatatcaagc cacgccagta aaagaagtaa aaatagaaaa tgaattaaaa cgtacgatgg
    19501 gattttttac tgctttatcg actgttatgg gcacagtgat cggtgcaggg gtatttttta
    19561 aagccgcaag cgtagcagaa gttacaggat ccgccagttt gcatatgttt tcttggtttc
    19621 tcggtggtat gatttctgtc tgtgcaggac taacaggagc tgaactggca gctgctattc
    19681 cagaaacagg agggatgatt aaatatatcg aacggattta cggaaataca gcagcttttt
    19741 tattgggctg ggcgcaagtc gtgatttatt ttccagcaaa tgtagcagcc ttgtcgatca
    19801 ttttcggtac acagttcgtc aatctatttg gtctttccca gtcaatgatt gttcccgtag
    19861 ctgttacagc tgctgtatcg atcctgttga tcaatttttt aggttctaaa gctggagggg
    19921 cttttcagtc gattacgctt gtctgcaagc ttattccttt gtttgtgatc gtcattttcg
    19981 gtttgttccg acaagaagga gtagacttcc aactgtttcc gatccaagca ggagaaaatc
    20041 tttcattttt ctcagccttg ggagcgggct tgctagcaac gatgttcgca tacgacggtt
    20101 ggatacacgt aggaaatatt tcgggagaat tgaaaaagcc ggcgaaagat ttgccaaaag
    20161 caatttcttt aggaatcatc ggaatcatga tcgtttattt attagtaaat gcggtgttct
    20221 taagaaccgc atctatcgac ggagtcgcag gaaacagcaa tgcggcaagt gacgttgcaa
    20281 aaatgatttt tggcggcttc ggtggaagac tggtcacagt tggtatcttg atttctgttt
    20341 atggaaccat caatggctat acccttactg gaatgcgtct cccttatgtt atggcaaaag
    20401 aaaacaacct gccttttagc aagctctttg ctaaacttca tgataaaact aaagttcctg
    20461 tagccgcagg tatcttagag ctagtcatcg ctattggcat gatgatgatc ggtggttttg
    20521 atacactaac ggatatgctg atctttgtca tttggatttt ttatacgatg gtctttgttg
    20581 gtgtcattct tcttcgtaaa aaagaacctg atttgtttcg accatacaaa gttccaatgt
    20641 atccgtttat tccactagta gcaatcattg gagggacttt tatcgtatca agcacgctta
    20701 tcactcagac ctttttagct tcgatgggga tagtattgac acttgctggt atcccgattt
    20761 atctctattt aaaacgtaga tagctaaaac attcgaattt tattagaact gcacttctct
    20821 gccaaacaaa atattttatt taaatgagga gtacgtggaa gaaatgtcaa aagtactcgt
    20881 taattttgat cacgtaaatg tcgttttgtc tacgatgaaa tagcaaagta aaagacagaa
    20941 atcgggctca accgatttct gtcttttact ttgatttgcg taactgaact attcggcgta
    21001 cgccgcgata aatatttaat aaaaccaagc aaccagaaat gaaaatggga agccaaatca
    21061 gatagacagg gtagccaagc actttagaca aagcatgtgg agtcatttta tccatttcaa
    21121 gaagtgcttg ctgccaagtc tgaagattga agactagcat gaaacccgaa ataatcagga
    21181 gaataaatcc taaataatga attgtagttc tttttcttac ataagcgatc gtagcataaa
    21241 ggaacagtaa aaaaataggt acgccaaaaa caaagtacat attcagcatc tcgacacccc
    21301 ctttgtgttt atcttacttt actttgaaag aaatgagaaa taatagcctc gttacttgct
    21361 tgaaacagca aaggactgct aagataagag agatacaaag aatcaaggag gagtcatatg
    21421 gcaagttata tcgaaccgat tcgttttgcg ctgattgttt ttccattttt agccttagcg
    21481 atctcatctg ttttttttat ttatgaatat cgaaagtatg gaacatttct agtaacaagg
    21541 gcagttattt tgtattcttt cgttttttat ttattgtgtg cgtatttttt ggtgatctta
    21601 ccacttccgg caaaggagac ggttgcccaa atgacagggc caaagatgga gctgcattta
    21661 tttgcttcat ggcaacattt cagagacaac acggtattgg tcttgtctga acctagtacc
    21721 tacctgccgg caatgaagca gagtgtattt ttggaaccag cttttaatgt tttattactc
    21781 ataccatttg gcatttattt aagatattat ttccggcttt cttttatgaa aacgatgatt
    21841 gctagtttct gcctttctct attttttgaa ttgacgcagc taagtggact ttattttatc
    21901 tacccaagac cataccgctt atttgatgtg aatgatttag tgacaaatac attgggtgga
    21961 atcattggtt ttgtcgttac gccgttattt acatttatgc ttcctacaag aagacgaatg
    22021 gatgaacttt cttatgaaaa aggaaagact gtctcattga ctcgacgctt ggtcgcttat
    22081 ttgatcgact ggacattttt atctcttatt aatttggtag cggcgatttt ctttaaaatt
    22141 gcgttcgatt caggggcagt tacttctcat atctggtggc ttttattaga agtaggggta
    22201 tactttatga ttgtaccgtt tataaccaat ggatggacac ttggcaaaaa aattgtgcgt
    22261 atccgtttgg tggaagaaga ccgcgaacga atttctttaa aagcattgat gatccgctac
    22321 gggtatctgt actttctttt ttatggttta agctggttgg ggcagttgct tcagcaaatc
    22381 atgaattcga caacgaatac ggtgttgcag atgagtgcag tattcatttc tctcggtgtc
    22441 ttgttactac aaggtatttt tattttaaat atcttgctat catttttgag aaaaaaacgg
    22501 attctgtttt atgaaaaagc tagtcacaca tatactgtta gcacgattgg ccaaacaaag
    22561 gatgatgatt ctcgaaatta gctgagcaaa aacgctttgt ctgtgttata atgccaaagt
    22621 cgtgataatt taaagagaag aggtgcacga atgaataatt ttgatatatt ccgcttcaaa
    22681 ccgtttatca atgaagcgtt aaccgcaaag ggcttcaaac agcccacaga ggtccaggaa
    22741 cgtttgattc ctttaattgc aaaaggaaaa aatatcattg ggcaatctca aactggtaca
    22801 ggaaaaacac atacgttttt gttgcctttg tttgaaaaaa tcaatgcaga aaaagaggaa
    22861 gtacaagttg ttattacagc tccaagtagg gaactagctg cacagatcta tgaagcagct
    22921 acacaaatcg ctaaattttc tacaccggag atccgtgtct cgaattttgt cggcggtaca
    22981 gacaaacagc gtcaattaaa tcgtctgcat caccaacagc cacaggttgt agtaggcacg
    23041 cctggtagaa tattagacat gatgaatgaa caagcgctga agattcacac cgcatttgca
    23101 tttgttgtcg atgaagcgga tatgacattg gatatgggtt ttttaaatga agtagaccaa
    23161 attgcaagtc gattaccaga aaagctgcag tttttagttt tttctgccac tattccagaa
    23221 aaacttcgtc cattcttacg aaaatacatg gaaaatccaa tcattgaaga aatcaaacca
    23281 aaagcagtta tttctgaaga cattgaaaat tggctgattt caacaaaagg taaagatgag
    23341 aatcaaatga tttatcaact gctgacaatt ggtcatcctt atttagcaat cgtatttgcc
    23401 aatacaaaac agcatgtgga tgaaattgcc gattacttga aaaaccaagg cttgaaagta
    23461 gcaaagattc atggagacat caccccaaga gagagaaaac gagtgatgcg acaagtacag
    23521 aatctggagt atcaatatgt tgtagcgact gatttagctg ctagagggat cgatatcgaa
    23581 ggtgtctctc atgttattaa tgcagaaatt ccagaagatc tagacttctt tatccatcgt
    23641 gtcggacgta ctggacgtaa tggcttaaaa ggaacggcca ttacgttata ttcgccaaat
    23701 gatgagcaag ccattgatgc gatagaaaaa ttgggcgtct catttgaacc aaaagaaatc
    23761 aaaaacgggg aaatcgtcaa aacatacgat cgtaatagac gtgccaaacg agaaaaatca
    23821 aaagatgtat tagatcccac gctgattggt cttgtgaaga agaaaaagaa aaagatcaag
    23881 cctggttata agaaaaaaat cgattgggca attgcagaaa aaaataaaaa agaacgcaaa
    23941 atcgaacgca gacagcaaag ccgtactgct cgtaaaaata agaaaaattc acataaataa
    24001 aaaacaagag gttgtgacaa aaggcgtgtc acaatctctt gtttttaagc tagacttttt
    24061 ttcattccga tacaaagtac ttcatcttca ataaaaggtt ctgtgcaagt atgataccct
    24121 tgtttttcat aaaaatctac agccgtcatt tcagctacta cataactagt ctgacaacct
    24181 tctgaaaccg cccgtttttc agcagcttca agcaactgac cgcctaaccc ttttcctcgg
    24241 tattcttttg ctacgcaaaa tcgttccggc ttgaagcaag taggtgtttt cttttcataa
    24301 cgcaaagtag ctactggtct tttttgatca agaaatagat aataggtttg ttgcgggata
    24361 tcttttccgt cgaattctag ttctgcagga atcccttgtt ctaatacaaa aacttgatat
    24421 ctcaaaaagc agctagctgc gcgtatccat tcatccgctc caactatttt tttcatcata
    24481 ttcctccttg tcatgaatag cagagatgta ttgattcctt tattcttatc gtaccaaaaa
    24541 tcgtggagta tgaccaaaag gaaatgaaaa taaaaattga gatattgaca gtgatcaata
    24601 gaatgcgtat actaattaag tacgaaaagg atatgatttg ttttttaaga ctcacagaaa
    24661 attctttctt ggctgaaaaa agaatagtca ctaatcaaat tgctcccttt ttctattttg
    24721 gtagaaatac cgacgcagct cagcgttatc gagtttaaga ggtaatgatt acacatcatt
    24781 gcaatcaagg tggtaccgcg agttttcgtc cttggctgtg atggtgtttt tttgtattta
    24841 atttatagaa agcaggtaat gacatgaaac aattaagtag tgcacaagtt cgacaaatgt
    24901 ttcttgattt cttccaatct aaaggacata cgattgaacc tagtgcgtct ttgatccccg
    24961 tgaacgatcc aaccttattg tggatcaact ctggtgttgc aacattgaaa aaatattttg
    25021 atggttcagt tgtgccagaa aatccaagaa tcacaaatgc ccaaaaatct attcgaacaa
    25081 atgatatcga aaatgtcggt aaaacagcac ggcaccatac gatgtttgag atgctgggta
    25141 atttttcgat tggtgactat ttcaaaaatg aagccatcca ctgggcatgg gaatttttga
    25201 ctgatccaaa atggatggca ttcgatccag aaaaacttta tgtaacagtt tatccaaaag
    25261 atacagaagc aaaacgcatc tggcatgaag aagttggcct gccactagat cacattattg
    25321 atgtagaaga caacttctgg gatatcggtg caggcccatg tggtccggat acggaaattt
    25381 tctatgaccg tggagaagct tataacgatt tagcagaaga cgatcctgaa aactatccag
    25441 gaggagaaaa tgaacgttat ttagaaatct ggaacttagt attttcagaa ttcaaccata
    25501 agcctgataa cacatttgaa ccacttcctc ataaaaacat tgatacgggg atgggattgg
    25561 aacgcatggt ctcaatcgtt caagatgcgt caacgaactt tgagacagac ttgtttatgc
    25621 ctattatcca cgccgtagaa aagctaagca aacaagtaaa atatggtgaa aacccagtaa
    25681 cagatatttc ttacaaagta atcgcagatc atattcgcgc cctttcattt gctattggcg
    25741 acggtgcatt gccatcgaat gaagggagag ggtatgtctt acgccgtttg cttcgtcgtg
    25801 ctgtcatgca tgggaaaaaa ttaggtattg aacaagcatt cttatatcgt ttagtccctg
    25861 ttgttgggga aatcatggta agttattatc ctgaagtact tcaacaaaaa gaatttattg
    25921 aaaaagttat ccgtacagaa gaagaacgtt tcaatgaaac aatcaatgaa ggtttggaaa
    25981 tcttaaatca agtgatccaa gaagtaaaaa acgaaaacgg gacgactcta gcaggaaaag
    26041 acatcttcaa actatacgac acatatggct tcccggtaga attaacggaa gaggtggcag
    26101 aagatgaagg actgaaagtt gatcatgctg gttttgaaaa agaaatggat gcacaacgcg
    26161 aacgtgctcg ctctgcccgc agtactgaaa aatcaatggg ggtacaatct gctttattga
    26221 cagatatcaa agtagaaagt aaattcattg gatacacaga acaagaatca gatagtcaat
    26281 tattggtcat cgttaaaggt gagtctcttt tgaatgaagt cgcagaaggt gaagctcaaa
    26341 tgatctttga tcaaacacct ttctatgcag aaatgggtgg gcaagtagcg gatcaaggat
    26401 ggatcatgaa tgaagtaggc gaaatcgtag cagttgttac agatgttcaa aaagcgccta
    26461 atggtcaatt tatgcacacc gtagaagtaa aagcaccaat gagtgaagga caaacctatc
    26521 atctattgat cgatcaatcg atgcgcaatc ggatcatcaa aaaccataca gcaacccatc
    26581 tactgcacaa ggcattgaaa gaagtacttg gcgagcatgc aaatcaagct ggttctttag
    26641 tcgctcctgg acatttacga ttcgacttta cccactttgg tcaaatcact tcagatgaat
    26701 tagctgaaat ggaaagaatc gtcaacgaaa aaatctggga agctatccca gttgaaacaa
    26761 ttgaaacaga tatcgataca gccaaaaaca tgggtgcaat ggctttgttt ggtgaaaaat
    26821 acggacatga cgttcgagta gtaaatattg gcggctggtc aatcgaactg tgcggaggca
    26881 cacatgtagc aaatacagaa aacatcggaa tcttcaaaat cgtttctgaa tcaggaattg
    26941 gtgcaggcgt gcgacgaatc gaggctgtaa caagtaaaga agcgtacgaa ctacttgaag
    27001 aagaagaaaa acaactaaaa caagtagcaa caattgttaa atcacctcaa ttgaaagaag
    27061 ttgtttcaaa agcggaacaa ttgcaacagc aagtaagaga gctacaaaag gaaaatgaac
    27121 aattagctag caagttagcg aatcaacaag ctggtgatgt attcaaagat ccagaaacga
    27181 tcaatggaaa aacaatcatt gcagcacaag taaaagtgaa agatatgaat cagctgcgtc
    27241 aattagctga ccaatggaaa caaaaaaata tttcagatat tcttgtttta gcaaacgaac
    27301 aagaaggaaa agtcagtctg ctagcggcaa tgacgaaaga atcaaatgaa gctggactaa
    27361 aagctggcga cttgattaaa gcaatcgcac cgaaagtagg cggtggcggt ggtggacgtc
    27421 cagacatggc acaagctggc gggaaaaatc ctgctggtat tccagatgca cttaaagctg
    27481 cgaaagaatg ggttgcgcaa caatagggcg tttacgtttg acagaaaaac aaaagttcaa
    27541 acattgaacg aaacatgatt atgggggcga agacaaacaa gtcttgccct catacttttt
    27601 tcaattggat ggcgcatcta atcaaatggt cggattcgga tggtctttag ttgacgaaag
    27661 tgacagaaat ttttacgatt agtataaata aaaaggggtg aaaaaatgaa tagtagaaat
    27721 aagacctttc gactagtctt acgtgcgatc ttgttagcaa tcatcatcgt acaagcgatg
    27781 gttccttggc tggggtttat tccattagga ttcattagtt taacaattat ccatatcaca
    27841 gtaattattg ctgcagtagt attaggccca aaagatggca tggtgattgg cttgttttgg
    27901 ggaatcgcta caattgttcg tgcctatgcc atgccaacaa cgccatttga tacattagtg
    27961 ttcacgaatc cgattatctc ggtagttccc agagtacttg ttggcttagt agcaggcttg
    28021 gttttccact ggatttatca aaaaaataaa tcaataacga tcagctcagc ctttgcaggg
    28081 gtactgggtt cattggtcaa tacagtctta gtgttaggat tcatgggatt attttataca
    28141 ggtgcaacgg cagatgccta tggtgtagac tctgctcttt tatttaaaac cttagcaggg
    28201 gttgcagcaa tcaatgggat accagaagca ataggtgcgg gaatcataac accattacta
    28261 gcaaaagcgt tatttgcagc aacaccgttg aaaccagaat aatgaccatc ttttaatagt
    28321 aataggacgc acgatagata gaaaaaggaa gagaggagag gaaaacaatc gtcctctcca
    28381 tctttttacc tgagcaacaa gcaaactaga aggggtaaaa aacttggcta agaaacaaga
    28441 agaaaaaaag aaacaaatac cgcaacagcg gacaaaaaaa ggagtactag cgggtatctt
    28501 aggattggtt acgaatctga tcttgtttgt ctctaagcta atgatcgggc ttttttcagg
    28561 aagtgtctcc attatggctg atgcagtgaa cagtctatca gatactgctt catccatatt
    28621 gactttgatc ggatttaaaa ttgctgcaaa gccagcagat caggaacatc cttatggaca
    28681 cgaacgcttc gaatatatca gtgggttatt tgtttcgatc atcattactt atgtaggatt
    28741 tcaattttta gagtcttctg ctgaaaaaat catccatcca gaaaatgtct ctttaacacc
    28801 catcgtcttt tttgtcctaa tcatttccat ctttttgaaa tttgcacaag gcaaaatgta
    28861 tcaggttatt gcgcaatcca ttgattctga aacattgaga gctacagcaa cagatagtta
    28921 taatgatgtc tttactacac tggctgtatt gctttcagca gcaatcgaat gggtaactgg
    28981 atggcgtatt gatggatata taggttttgc tttagcatta tatatccttt atagtggatt
    29041 gacgatgctt cgaaattttg tatacgaact attaggaagt cgcccaacgc cagcagagat
    29101 tgaagaaatg gaagagcatt taaatgcttg cgatgatata cttggctatc atgatttatt
    29161 ggtgcataac tatggaccca ataaacgctt tgcatcagtc cacatcgaag tagatgaatc
    29221 aatgaatttg aatgtggctc ataagattat tgatctgata gaaaaagact ttaaaaagtc
    29281 tttggatgtg gaacttgtct gtcacttgga tccaatgcct gttgataacg aaaaatacta
    29341 taatatcttg cggcagttaa agcaaattct tcttgaaaca ggtacagggc ttaatattca
    29401 tgattttaga gtaattcatc aaggaaaaac gctgcaattt gatgtagtgg tccctgagaa
    29461 attccagttt gttgatacag aactagaaag aatcttacgt agtcaaatag aagaaaaaat
    29521 cggagattac caattggaaa ttacgtttga tcacaattat cttttatatt aaatgagaaa
    29581 ctataaaaag actccttaaa gtttttaatt taaagagtct ttttttcttt taattgttta
    29641 tggagagcct gacgtgcaag attatctgct cctttatttt tgttttcggg caaccattgc
    29701 aggatcaata aagaaaattc ggatagaagt tcttggattc tttgaaggta gggaaggaag
    29761 agctcgttct tcgttgcgtt tttgtcgacc gtagaaacta gtgttttgct gtctgaaaaa
    29821 cagaaaatcg tttcattctg taagtcgttt ttctttagaa attctaaagt ttttaaaaag
    29881 acttcgaact ctgcttgatg gtttgtccct atagaaagcg ggatagatag ctgttcttgt
    29941 ttaccttgat agacaatcaa gattccacct ccactaggac cagggttgcc ttttgtagag
    30001 gcatcaatgt atacttttaa cataaaaaga gattctcctt tttgttcaaa ctaatgttat
    30061 ggtaagatta tatcattact gaatcggtga gaggttagag aatgaaaaaa aatattcgat
    30121 ggcagcctga actggcacat acgatcatct attggtcatt gaccttgatc attctgtttt
    30181 ttagtctgat tctttcttta gaaaacacag gtccttattg gaaaagcaat tttgttttag
    30241 gtatttttct tgtttttcta tttctaggtt tgcggcgttc ttttatttta aaatcaaact
    30301 ctgtgattat acgttatgct gctttttgga aagaccgaga aatatctgtc ggacagattc
    30361 gatctttgac gccaactaat actggggtgc aaatgatgtt gcaagatgag aaagccccat
    30421 ctgttttcat gatgcgaaaa aaagatcaag tagtcctttt tgatcatttt gaaaaaaacg
    30481 gacccaaatc agaatcaatt ccttatcctt atacgaaaaa aagaaaaagc taattttaaa
    30541 aatcagaacc taaaaaaacg aatttttaca ggaaaagaaa agagtgagac gtacggttca
    30601 tcccgtttag cgtctcactc tatttgttta tctattcatg catactagta tcggtagtct
    30661 gaacgttgcc tttcttgaca tgggcagctt gtgttcctcg attcccttcg atgatctcaa
    30721 attcgaccac ttgattttct tccaaagttt tgaatccctc ttcttcaatc gcagtaaaat
    30781 gtacaaaaat ttcttctgtg tcatcataac tgataaagcc gtatcctttt ttgttgtcaa
    30841 accattttac aattcctgtg gtcaaagcac tcacgacctt tctaaagtct gatcatagcc
    30901 ttcattataa agagtattta taagtacgtc aaataaatta aaaacctgta attttatgtt
    30961 ttttttctga ataaaaaaag gtataattgg catatcttaa caaagaagat attttgttga
    31021 ataaagttcg tgaaaagggg taatcaaatg aagaatgatc tgcaaatttc acaagaaaca
    31081 gagctagaac cgattgttga agtggctagt aaattagatc ttaaggaaga cgatttggaa
    31141 ctgtatggga aatataaggc gaaaatcaat ttttctggaa tcaatcgatc aaaggataac
    31201 gctgaggggc atttgatctt agttacttcc atcaatccaa caccagctgg cgaaggaaag
    31261 tcaacgatca ctgtagggtt aggagacgcg ctcaataaaa tcaataaaaa atctgttatt
    31321 gcattgcgag aaccctcact tggtcctgtg atgggaatca aaggtggtgc tgcaggcgga
    31381 ggatatgcac aggtattgcc tatggaagat atcaacttgc attttactgg tgacatgcat
    31441 gctatcacaa cagctaataa tgcattatct gctcttttag ataatcatat tcatcaagga
    31501 aatgaattga acatcgatgc ccgccgagtg atttggaaac gggtagtcga tctcaatgat
    31561 cgtgaactgc gtaaagtaat tgttggcttg ggtgggccaa tccaaggtgt tccaagagaa
    31621 gatgggtttg atatcacagt tgccagcgaa atcatggcta tcttgtgtct tgcggcagac
    31681 cttgaagatt taaaggctcg tcttgcaagg atcgttgtcg gctatacatt tgaccgtcag
    31741 cctgtgactg ctggagactt gaaagcagaa ggcgctctgg cattactgct gaaagatgca
    31801 atcaagccga atcttgtaca aacaatttac ggaacaccag cttttgtcca cgggggaccg
    31861 tttgccaata tcgctcatgg ctgcaatagt attttagcta ccaagacagc attgagatta
    31921 gcagattacg ttgtcacaga ggcaggattt ggtgcagatc ttggcggaga gaaattttta
    31981 gatatcaaag tacctaactt gaaaaaagcg ccggatgctg tggtgattgt cgccactgtc
    32041 cgtgcattga aaatgcatgg cggaatgaaa aaagatgaac ttaaaaatga gaatcttgat
    32101 gctttgaaag taggttttgc aaatttaaag cgccatatac gtaatatgga acaatatcag
    32161 ctacctgtga tcgtcgcaat caatgaattc gtaacagata cagacaatga actcacactg
    32221 ttggagcact tgtgtgaaaa tcaggggatt cttgccaaga gagcgagtgt atgggcagat
    32281 ggagcagaag gcggcgttga tttagcaaaa gctgtagtac gattgattga tagaaaagaa
    32341 gctaattaca agccattata ccgcctagaa gaaacgatac aagaaaagac agaaacaatc
    32401 gtcaagaaaa tttacggagg aaatggtgtt gttttcagca aaaaagcgca aaaacaaata
    32461 gaagagttta cgaagaatgg atgggacaaa ctgccgattt gtatggcaaa aacacagtat
    32521 tcattttctg atgatccttc actgctaggt gcaccggaag atttcaccat cacgatccgc
    32581 gaaatcatcc caaaattagg ggcaggattt ttagtagcct tgactggaga tgttatgacg
    32641 atgcctggtt tgcctaaaaa acctgctgca ttgaatatgg acgtgacaaa tgacggagaa
    32701 gtgttgggat tgttttaagc ttgattataa tctaccatcg aaggagctgt attttgacta
    32761 cagcttcttt ttttagacga tagggaatta attttgttta cactaggatc attctttttc
    32821 tttattgtat tttgagctat actaaagcat aataggaggg tatgatgaaa acgacattta
    32881 taagaaaaac cggtttttac ggtatgggta gctcgattca gctaagaatc aacaatgaaa
    32941 aacgcagtgt tctcagtcat gaacaaaaga taacgcttga tctggaagtg ccctttacga
    33001 tgcaggtcac cttttattgg ctaaaaagcc ccgtttatac agtgaaagaa gcaaagcctg
    33061 cttatgtgat cacaatgaat tacttgattc ttcaaatcta tccgttttta tttctagcaa
    33121 ctgggttggg ggccgttttc ctacaaaatt tatttttcag tttttttgca gttattgtgt
    33181 tgattgcgtt ctttttctat ataaaaaacc gggcatattt gatcaaggag gaaaattatg
    33241 gcaaactctg atcttttttt aatcagtttc aatcgaatcg aaaaatggat gagagacgag
    33301 atgggaaatg cacgaaatat gggatttacc gaacttgtca gacgcctagc tcaaagaaaa
    33361 caattaatga tcagaaaata cgaagatgat cttttacagc ttgctcagtt aaggaatgcg
    33421 atcgtccatg atcggatcgc agtcgatttt gtcattgcag agccaaatga ttggacagtc
    33481 aagcgaatcc agctgatcga acaagaactg attcagcctg aaaccgtgct gcctcgattt
    33541 gcaaaacgtg taacaggttt tgaacaagat cttccgttac ttgaactgtt aaagatagta
    33601 gcagaaaaaa ggtattcaca atttccgtta tacaataaag gaaaatttgt agcattgatt
    33661 actttacgta atatcgggtt ttggctagca aaagaaagcc aaaaaggtcc ggttgattta
    33721 acaaataaac gagcgttgga tttgatcatc caaaacggaa agtacacaaa ttatcatttt
    33781 gttccagcta aaacacatat ttacgaggtt gaagcgatgt ttcgtgagca agggactttg
    33841 gaagccgtac ttatcactaa agatgggaat cccgatggta atctgctcgg gatcgtacga
    33901 cctcgtgata tttataaaca aatagaaaag gattgatttt ttgttaatta tttattggat
    33961 attcagcgct ttgctagtag gagtggatca gttggtcaaa tggtggatca ctgataatct
    34021 agcgcttgga gagacgaaaa atcttatacc aggtattttg tcactgaatc atattcgaaa
    34081 cactggagcc gcatggagta tgctagaagg gaagatgtgg ttctttacta tagtgacact
    34141 cattgcagtc gttgtgattc tcacattgat gattaaaaat cgtgaaaaag gtaatcgctg
    34201 gttcatgatt ggattgagtt tgattctagc tggtgcgatt ggcaatttta tcgaccgtat
    34261 tcgtcttggc tatgtagtag atatgttcca gacagatttt atgagctttc cgatttttaa
    34321 cgtagcagat gtgacacttg tatgtggagt gatttgtatg ttaatttata ttattttaga
    34381 tgaaaaggat caaagaaaaa aatgacagaa cttaaaacga cgattcacca tgaatctggt
    34441 aggatcgata aagtattgac taatttattc accgattaca gtcgttcgca aattcagctg
    34501 tggctgaaaa atggagcggt ttctgtcaat ggacagccag tcaaagcaaa ttacaaagta
    34561 aaacaaggag acgagattac tgtcttgatc cccgaaccag aaacattaga tgtggtccca
    34621 gaaaatatcc cgttgacgat cgtttacgaa gatgaagatg tagcggtgat aaacaaacct
    34681 caaggaatgg tcgttcatcc ttcagcaggt catacgcatg gcacaatggt caatgcgctg
    34741 atgtaccata tcaaggatct ttctacgatc aacggcgtta ttcgtccagg tattgttcac
    34801 cgaatcgaca aagatacttc cggactgcta atggttgcca aaaatgacca ggcacacgaa
    34861 tcattagctc agcaattaaa agataagacc tctttgcgca aatatgttgc tttagtccat
    34921 ggagagattc ctcacgaaaa aggaaccatc aatgcgccaa tcggccgttc gaaggaaaac
    34981 cggaaaatgc aggcgataag agaagatggg aagtcggctg tcacacattt tactgtattg
    35041 gaacgtttcg aaggatttac tctggttgaa ttaaaactag aaaccggaag aacacaccag
    35101 atccgtgtgc atatgaagta tatcggttat ccgttagctg gagatccagt atacggaccg
    35161 aagaaaactt tgaaaggcaa tggtcagttt ctccatgcgc aaatgttagg ttttacgcac
    35221 ccgactacaa acgaaaagat gatctttgaa gcacctcttc cagaagtttt tgaaaaaact
    35281 ttagaaaaat taagaaaaaa cattgcattt taatttagat aacggtatag taattaacaa
    35341 cgaaagcata aaacttaata gaattccttt aagtttagtc ccgtgaggct aagaagggtg
    35401 ggagcaagga tacgtatggt gcgccataag tagattgctc attgacatag tatagctatc
    35461 agtgtatcag tatacgctca gtctaaacat tcctcctgtc ctcttagggc aggagttttt
    35521 ttatgcaaaa aagtaggggg aatgaaaatg caagcaaaag aagtagtaga tcaggtaacc
    35581 atgaagcgag cattgactcg aattacttat gagattatcg agagaaacca tagtatccaa
    35641 gatatcgtat tggtaggtat caaaacacga ggaatctaca ttgcatcaag aattgccgaa
    35701 cgcctgaagc agctagaaga tatcgatatt cctgtaggag aactggatat cactttatac
    35761 cgagatgaca aaaaagaaaa tccagaagaa ccggaacttc attcttctga catcccagtt
    35821 tccttagaag gaaaagaagt tattttgatt gatgatgttt tgtatacagg acgaacgatt
    35881 cgagcagcaa tggacgcagt aatggatttt ggcagaccaa gaaaaatttc attagcagtc
    35941 ctggtcgatc gtgggcatcg agaattgcca atccgtgcag attatgttgg gaagaacatt
    36001 ccaacatctc gggcggaaga gattttagta gagatgcaag agcttgatgg acaagatcgc
    36061 atcatgattt tgaaagagga agattagagg agagagaaac atgtcggaga aaaaaggatt
    36121 tcataatgat gacgtggtgt tagatatcca cgatcgccca accccaggac attgggtagg
    36181 attaagttta caacacttgt tcacgatgtt cggagcaacc gtgttagtcc ccatcttagt
    36241 aggtatcgat ccaggtattg cgctagtcag ttcaggacta ggtacattgg tgtacttatt
    36301 tactactaaa ggaaagatac ctgcgtactt aggaagcagc ttcgctttca ttgcagcgat
    36361 gcagcttttg atgaaaacag atggttatcc agcgattgca caaggagcag tcacaactgg
    36421 attagtctat ttgattgttg cgctgatcat caaaaagatc ggttcagcat ggctggataa
    36481 aatcttgcct ccaatcgtcg tcggtccagt catcatggtt atcgggttgg gacttgcggc
    36541 aaatgcagca acaaatgcta tgtataatca gggcacgtat gatttcaagt atattgctgt
    36601 agcgctgatc acattaggat taacaatttt cttcaacatg tggctaagag gttttctcgg
    36661 attgattccg attttactag gtattattag cggatacatc gtagccttgc tgtttggaat
    36721 cgtggatact aagccaatcg tagacgctgc ctggtttgct gttcctaact ttgagattcc
    36781 ctttgtacaa tatgaaccaa agctatatct tggagcaatc acaacaatgg cacccatcgc
    36841 ttttgtgacg atgacagagc atatcggcca tcttatggta ttgaataaac taactaaacg
    36901 taatttcttt gaagaaccgg gtcttcataa gacgttgaca ggtgatggat tagcacaaat
    36961 cgtggcagcc tttgtcggag gtcctcctgt aacaagttac ggtgaaaaca ttggtgttct
    37021 ggctatcaca cgtgtccata gcgtgtttgt gatcggtgga gctgcagtat ttgcagtcat
    37081 tttaggattc gtaggaaaat taagcgcttt gattttaagt atccctggac cagtcatctc
    37141 aggaatcagt tttatcttat ttggagtaat tgccgcaagt ggaatgaaga ttttaatcga
    37201 gaacaaaatc gattttgata aaaagaaaaa tctgttgatt gcttcagtta tcttagtagt
    37261 aggaattggc ggactcgtct ttgaagtagg cacatttaca ttgtcggcaa tggcattagc
    37321 aactgtactt ggcatcattt taaatcttat cttaccagaa acttcacgaa gcgaagaaaa
    37381 ctaaggagga aacattatga tcatcaaatc agaacgtatc agcttaaagc atttattgac
    37441 cgttgaggca cttactgatc aagaagttat gggcttgatc cgcagagggc aggaattcaa
    37501 gcaaggtgct aattggactc cgcaaaagcg ccagtacttt gctacaaacc tctttttcga
    37561 aaatagtact cgcacacata aaagcttcga tgtagcggag aaaaaactag ggctagaagt
    37621 gattgaattt gaagcaagca caagctctgt ccaaaaaggg gaaacactat acgatacagt
    37681 actgacgatg tcagcattag gcgtagacgt agcggttatc cgtcatggtg atgagaacta
    37741 ttatgacgaa ctgatacaaa gtaaaacaat ccagtgttcg attatcaatg gaggagacgg
    37801 aagcggacag catccaaccc aatgcttgct tgatttgatg accatttatg aagaatttgg
    37861 tcattttgaa ggtctgaaag ttgccatcgt aggtgatatc actcactctc gcgtagctaa
    37921 atccaatatg cagatgttaa aacgtctagg cgcacaagta ttcttctcag gtcctagaga
    37981 atggtacgac gatgaattcg aagtgtacgg acattatatg ccattggatg aactactaga
    38041 tcaagttgat gtcatgatga tgcttcgggt gcaacacgaa cgccatgatg gaaaagaaag
    38101 tttctcaaaa gaaggctacc atcaggaaca tgggttaaca gttgagcgtg cgaagaagtt
    38161 gcaaaaacat gcaatcatta tgcacccagc accagttaac cgtgatgtag aattagctga
    38221 ttcattagtg gaaggattac agtcgcggat cgttcagcaa atgagcaatg gagtattcgt
    38281 cagaatggca atcttagaag cagtgctaaa tggaaaagcg taattatagg aggatgaaaa
    38341 aatgaaaaca ctcattaaaa acggtcagat caatacacga aaaaatatga cgacaccagc
    38401 agaaatttgg atagaagatg ggcgaatcaa agcaatcggt acaggattct cagaagccga
    38461 atttgatgaa gtgtttgatg caaagggaca attgatcaca cctggtttag tggacgtcca
    38521 tgttcatttg agagagccag gatttacata caaggagacg atcgaagctg ggactcgctc
    38581 agctgctcgt ggcggtttca ctactgtatg cgcgatgccg aatttaaatc ctgtacctga
    38641 tacggcagaa aagctgagac aagtatatga catcattaga aaagatgcag ttgtgaaggt
    38701 attgcagtat gcgccaatca cagaaaatct gcgaagtgag aaattagttg atcaagaagc
    38761 actgattgaa gaaggtgctt ttgctttcac aaatgacggt gtaggtgtcc aaacagctgg
    38821 gacgatgtat ctggctatga aagaagctgc aaaaaacaat aaagccctag tagcccatac
    38881 agaagatgaa tctttattat ttggcggcgt tatgcacgct ggcaagaagg cagaagaact
    38941 tggtcttccc gggatcttaa gcgtaaccga gtcttctcaa attgctagag atctgttgct
    39001 agctgaagca actggtgttc attaccatgt ttgccatgtt tctactaaag aaagtgtccg
    39061 tgtaatcaga gacgcaaaaa aagcaggaat ccacgt
//
LOCUS       DACEEZ010000017        36302 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20313_2017
            SAMN12639966-rid9879223.denovo.017, whole genome shotgun sequence.
ACCESSION   DACEEZ010000017 DACEEZ010000000
VERSION     DACEEZ010000017.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639966
            Sequence Read Archive: SRR10023158
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 36302)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 36302)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741683.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741683.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:44:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,016
            CDSs (total)                      :: 2,962
            Genes (coding)                    :: 2,889
            CDSs (with protein)               :: 2,889
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 36 of 73
            Pseudo Genes (incomplete)         :: 29 of 73
            Pseudo Genes (internal stop)      :: 18 of 73
            Pseudo Genes (multiple problems)  :: 8 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..36302
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639966-rid9879223.denovo.017"
                     /isolate="E20313_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(<1..1858)
                     /locus_tag="IWO89_000947"
     CDS             complement(<1..1858)
                     /locus_tag="IWO89_000947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide cleavage/export ABC transporter"
                     /protein_id="HAQ0455719.1"
                     /translation="MIKSFPFVQQQDEKDCGVACLSMILKYFKTDIPIHKLRDLSGTD
                     LEGTSAFGLKKTFEKLSFDCMAIQADSEIWKERDLILPLIAHILVDNSYMHYVVIYKT
                     KGNVLWIADPANGKNRKTIEAFQKEWTGILLLPMPRETYIPIKEKVAGLSSFLPIIWK
                     QKGLVFHIVLASIFITFFGIGSSYYFQGILDFFIPNQARSTLNIISIGLIAVYLFRVL
                     FEYSRSYLLIILGQRMSMAIMLQYFKHVLTLPMNFFATRKSGEIISRFLDASKIIDAL
                     ASATLSIFLDIGMVLLVGVTLAIQNSTLFLITLCSLPFYVVAIFAFVKSYEKSNREEM
                     AAGAAVNSSIIESLKGIETIKAYSGEEKVYNRIDHEFVKLMKKSFRTVTLDNIQQGIK
                     HLIQLISSALILWLGSYFVMDNRISLGQLITYNALLVFLTDPLQNIINLQVKMQTAQV
                     ANNRLNEIFAIDSEKSNQSFEYMLSEQLFQQDITVKNVSFSYSMKAPTLNEISCTIPS
                     HSKTAIVGVSGSGKSTLAKLFVRFYEASEGSIHYGNINHLDIPFQILRDRVTYLPQES
                     FFFSGTIIENLIFGLDSPPSFEQIIEVCEIVQLNEFINQQPLRYETLLEEGGN"
     gene            2116..2352
                     /locus_tag="IWO89_000948"
     CDS             2116..2352
                     /locus_tag="IWO89_000948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin"
                     /protein_id="HAQ0455720.1"
                     /translation="MFKHAKCKGTKYCGNAQTVGGGNNAWGKLGQVVGGLTTGAVGGA
                     GLGTAICGPACGVVGGLYGAVAGGAAAGWDARKK"
     gene            2371..2586
                     /locus_tag="IWO89_000949"
     CDS             2371..2586
                     /locus_tag="IWO89_000949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin"
                     /protein_id="HAQ0455721.1"
                     /translation="MHIKNTKTTFILSSEELKNIQGGSAVGVLGTTFSGATAGVKLCS
                     AGGPYAIAACGVGGALLGAGFSMWTGA"
     gene            complement(3289..4041)
                     /locus_tag="IWO89_000950"
     CDS             complement(3289..4041)
                     /locus_tag="IWO89_000950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAQ0455722.1"
                     /translation="MSYPIILCEDQIIQLNQLERIIDNFILFHDKVFKIVLKTQSPLE
                     VKKYLKQFRPKQGIYFLDIDLNHEVNGIELAEVIRKYDVQAKIIFTTTHDEMLPVTIK
                     RRVETLGFVTKDQTLDEYRNEIVELLLLAQERIDATKQVTNQAFVFSIGSQTFTFDIN
                     DIYFVEASKLPHRLSLCTKDGQYEFYGRISELEKKYPMLTRISRACLVNIFNVKEIDF
                     KKRSLYFDSELARNFTLGKAQKIKEKLKESTV"
     gene            complement(4056..5303)
                     /locus_tag="IWO89_000951"
     CDS             complement(4056..5303)
                     /locus_tag="IWO89_000951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HAQ0455723.1"
                     /translation="MFVGNFFFFLLFYMTNTDLNKRKYLFVCLLIPLTFFVELFTDVS
                     AAIPIIGSYFILKEKKKTNIVLLNKLLLCMLIDDFRSIITSMVMTYMFSFEGIKGYGY
                     VVFQIMLDSLFLLMFIWLYRKLAINRIVEQSSSKLTAIFMIYLLTIGLFVSYVAHQYQ
                     VFDHFVFGVLIFLIIQMFVVLFLFIRITIRQREQYEQQLKRKELVYLKKYTDSLEKDQ
                     EKFARFRHDYKNLLLSLKEIANQNHDSILEKEIKELEIYSNSYLTNKFEYRYLKNIKN
                     EYLKSLLIAKLYQANNNKICCKFECPSTIIEVPVPIFDCIRVLGIILDNAIEASIESQ
                     DRELSLAIYRDDKQIEISISNSCREILLSIDTLMKKGITTKKGHQGLGLSSIEEINKK
                     NPNMFVNYQNDPYQFTTQVILLF"
     gene            complement(5379..5525)
                     /locus_tag="IWO89_000952"
     CDS             complement(5379..5525)
                     /locus_tag="IWO89_000952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EntF family bacteriocin induction factor"
                     /protein_id="HAQ0455724.1"
                     /translation="MEEKNRLNAKQCSDQELKKIKGGAGTKPQGKPASNLVECVFSLF
                     KKCN"
     gene            complement(5629..5940)
                     /locus_tag="IWO89_000953"
     CDS             complement(5629..5940)
                     /locus_tag="IWO89_000953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin immunity protein"
                     /protein_id="HAQ0455725.1"
                     /translation="MKKNAKQIVHELYNDISISKDPKYSDILEVLQKVYLKLEKQKYE
                     LDPGPLINRLVNYLYFTAYTNKIRFTEYQEELIRNLSEIGRTAGINGLYRADYGDKSQ
                     F"
     gene            complement(5942..6139)
                     /locus_tag="IWO89_000954"
     CDS             complement(5942..6139)
                     /locus_tag="IWO89_000954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enterocin"
                     /protein_id="HAQ0455726.1"
                     /translation="MKHLKILSIKETQLIYGGTTHSGKYYGNGVYCTKNKCTVDWAKA
                     TTCIAGMSIGGFLGGAIPGKC"
     gene            complement(6533..8260)
                     /locus_tag="IWO89_000955"
     CDS             complement(6533..8260)
                     /locus_tag="IWO89_000955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HAQ0455727.1"
                     /translation="MNNLLNYQLPVANWVENITEWFTTTFSGLFSFLQTIGQAVMSGI
                     TNLLLVIPAPLFILLLTVAAFFISKKRPGLTLFTLIGLWFIYNQGLWNDLMNTVTLVL
                     LSSVISIIIGVPLGILMAKSSKAQSIIKPILDFMQTMPGFVYLIPAVAFFGIGMVPGV
                     FASVIFALPPTVRFTNLGIRQVPKELVEASDSFGSTSRQKLFKLELPLAKSTIMAGIN
                     QTTMLSLSMVVIASMIGAPGLGRGVLSALQRAQVGNGFVNGVALVILAIIIDRFTQHL
                     NQPNNKKAAGATTQKSKKRQGLIIGAVVVVILGAIGIGSFSSAKETKRINLSYVEWDT
                     EVASTNVVGEVLKQMGYDVTMTPLDNSIMWKSVSNGESDAMVSAWLPKTHGSQYAQYK
                     DQVEDLGANLTGAKVGLAVPAYMDVNSIDELTDQAGKKIIGIEPGAGVVTAAENTIQK
                     YDNLKDWKVETSSSGAMTVALGQAIKKHEPIVVTGWTPHWMFAKYDLKYLEDPENGMG
                     SEEQIHTMVRKGLKEDQPEAYKVLDNFHWSEKDMEKVMLEINNGKDPQQAAKDWIKEN
                     QELVESWKK"
     gene            complement(8253..9446)
                     /locus_tag="IWO89_000956"
     CDS             complement(8253..9446)
                     /locus_tag="IWO89_000956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine/L-proline ABC transporter
                     ATP-binding protein"
                     /protein_id="HAQ0455728.1"
                     /translation="MVKVEVKHLTKIFGKKTQAALDMMNDHQPKTEILKKTGATVGVY
                     DVNFDVKEGEIFVIMGLSGSGKSTLIRLLNRLIEPTSGSIYIDGEDVSKLSKEELREV
                     RRHKINMVFQNFGLFPHRTILENTEYGLEVRGVPKEERQEKAEKALENSSLLSFKDQY
                     PSQLSGGMQQRVGLARALANDPEILLMDEAFSALDPLIRREMQDELLDLQANVQKTII
                     FITHDLNEALRIGDRIALMKDGEIMQIGTGEEILTNPANDYVREFVEEVDRSKVLTAQ
                     NIMVPALTTNIESDGPNVALTRMRNEEVSMLMAVDRKRHLKGIITADQALEARKQKRP
                     LIDFLDENVTVIGKDMVVSDIFNIIYDSPTPLAVVEDGKLKGVVIRGSVIEALAETSE
                     VSEHE"
     gene            complement(9633..10277)
                     /locus_tag="IWO89_000957"
     CDS             complement(9633..10277)
                     /locus_tag="IWO89_000957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAQ0455729.1"
                     /translation="MSGKKKIVRPRYQQIAVDLAERIVENRYKVGEKIHARSTLASTY
                     NASPETTRKAINVLVDLGIMEVRHGSGAFVASKEKAKDFLLQYQDVQSIQDTKAEIMA
                     SVEKQQDELNHFSQLLDQLVNQTKRIHKMNPLLPFELSLSEKAMHLDRSISELNIWQA
                     TGATVIAISHNEELLVSPGPYAKFTAGDIVHFIGNEYTLQRMTNFFYPEHQLAE"
     gene            complement(10371..10505)
                     /locus_tag="IWO89_000958"
     CDS             complement(10371..10505)
                     /locus_tag="IWO89_000958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FeoB-associated Cys-rich membrane protein"
                     /protein_id="HAQ0455730.1"
                     /translation="MATFLLSVLIFGSAGAIVYTRLKSGKNCDDCQTACPVKKEQIKR
                     "
     gene            complement(10507..12639)
                     /gene="feoB"
                     /locus_tag="IWO89_000959"
     CDS             complement(10507..12639)
                     /gene="feoB"
                     /locus_tag="IWO89_000959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein B"
                     /protein_id="HAQ0455731.1"
                     /translation="MNIALVGNPNSGKTSLFNLLTGSNQYVGNWPGVTVEKKEGSLKK
                     NPNIHIQDLPGIYSLSPYTPEEVIARDYLVQEQPELIINIIDATNLERNLYLTTQLLD
                     LDIPMIIGLNMMDVIKKEGKKINLEKLSYGLGVPVLPISVLKKQGIEKLIEKIQESAK
                     KRPSIPVPCTYDPRLEAALHEIEDVLGEQSGKKRWYAMKYFERDQKVREDYEIAVSQQ
                     KEIEQLIQLTEKLLDDDSETILVNERYEFITQLCTLCVVSNDSFQLSMSDKIDQIATN
                     RWLALPIFAFVMWLIYYLAIQTVGTMGTDWINDTLFGTWLPEHVGRLLAEWQVAGWMQ
                     ELILNGIIAGVGAVLGFLPQLIILFLCLSFLEDCGYMARIAFVMDRLFRKFGLSGKSF
                     IPMLIATGCGVPGVMASRTIENEQDRRLTIMVTTFMPCSAKLPIIALVAGAFFPHQSW
                     VAPSAYFLGMTAIIFSGISLKKTRLFAGDTAPFIMELPAYHFPQWSAVLRQTYDRSKS
                     FVKKAGTIIFVSSIVIWFSSSYNFYAQPVSEDQSILAFFGRILAVVFQPLGWGNWQGT
                     VAAITGLVAKENIIGTFGILFGHAEVSENGREIWQVLQHTYTPAAAYSLLAFNLLCAP
                     CFAAIGAIHREMNAKKWTWIAIGYQCGLAYLVSFVIYQLGHLLEGGQVALGTYLAILL
                     MIGLVYVLFRQPKSKNQFYTILSLEGEE"
     gene            complement(12636..13109)
                     /locus_tag="IWO89_000960"
     CDS             complement(12636..13109)
                     /locus_tag="IWO89_000960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein A"
                     /protein_id="HAQ0455732.1"
                     /translation="MQTLAQAKQNFVYQVERIETEEKTKRHLQNIGIVAGSKIVVVNL
                     SGNNAILLVKSHRVAVSREILGQIFVNKKTASSESWTSLDQLKAGETGIVVAIHGQGA
                     VKRRLMDMGLTRNVKIVIRKLAPLGDPIELTVRGYELTLRKSEAELILIQREEKE"
     gene            13510..13734
                     /gene="nrdH"
                     /locus_tag="IWO89_000961"
     CDS             13510..13734
                     /gene="nrdH"
                     /locus_tag="IWO89_000961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin-like protein NrdH"
                     /protein_id="HAQ0455733.1"
                     /translation="MNVKIFSKNNCMQCKMVKRFLTENQIAFEEVNIDEQPEAVDWLK
                     AQGFQSVPVITSDAETVIGFRPDQLRKLAS"
     gene            13893..16052
                     /gene="nrdE"
                     /locus_tag="IWO89_000962"
     CDS             13893..16052
                     /gene="nrdE"
                     /locus_tag="IWO89_000962"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit alpha"
                     /protein_id="HAQ0455734.1"
                     /translation="MSLKNLQDVSYFKLNNEINRPVNGQIPLNKDKEALVAFFEENVK
                     PNTMTFLSIMEKINYLIDQEYIEAEFIQLYSPSFIEKLYQFLDEQHFSFKSFMAAYKF
                     YSQYALKTNDGSAYLETYEDRVAFNALYFANGDEELALALADEMIHQRYQPATPSFLN
                     AGRKRRGELVSCFLTQVTDDMNSIGRSINSALQLSRIGGGVGITLSNLREAGAPIKGY
                     EGAASGVVPVMKLFEDSFSYSNQLGQRQGAGVVYLNVFHPDIIAFLSAKKENADEKIR
                     VKTLSLGVIVPDKFYELTRKNEDMYLFSPYSVEKEYGVPFSYVNITAEYDHLVANPNI
                     RKQKIKARDLENEISKLQQESGYPYVINIDTANRENPIDGKIIMSNLCSEILQVQTPS
                     VINGKQEYEVLGTDISCNLGSTNIVNLMQSPNFGQSVRTMTRALTFITDASDIDVVPP
                     IQNGNKLNHTIGLGAMGLHTYLAKEQMEYGSEDSLDFTNIYFMLLNYWTLVESNNIAR
                     ERKQVFHNFEKSAYADGTYFEKYVTGEFQPKSEKVKALFEGIFIPTAEDWNALKQAVM
                     KDGLYHQNRLAVAPNGSISYINDTSASIHPITRMIEERQEKKIGKIYYPAPYLSNETI
                     PYYTSAYDMDMRKVIDVYAMAQQHVDQGMSLTLFMRSEIPEGIYEWKTTTKQTTRDLN
                     ILRHYAFHKGIKSIYYVRTFTDDAEEIGSNQCESCVI"
     gene            16102..17067
                     /gene="nrdF"
                     /locus_tag="IWO89_000963"
     CDS             16102..17067
                     /gene="nrdF"
                     /locus_tag="IWO89_000963"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit beta"
                     /protein_id="HAQ0455735.1"
                     /translation="MSETYYEAINWNEIEDIIDKSTWEKLTEQFWLDTRIPLSNDLDD
                     WRTLSQLEKDTVGHVFGGLTLLDTVQSESGMDQLRKDVRTPHEEAVLNNIQFMESVHA
                     KSYSSIFSTLNTKKEIEEIFDWTNTNPYLQKKAERINEIYKHGTPLEKKIASVFLETF
                     LFYSGFYTPLYYLGNNKLANVAEIIKLIIRDESVHGTYIGYKFQLGFNELPEAEQETL
                     KDWMYNLLYELYENEERYTEELYDPIGWTEEVKTFLRYNANKALMNLGMDPLFPDTAN
                     DVNPIVMNGISTGTSNHDFFSQVGNGYLLGHVEAMKDDDYLIGLE"
     gene            complement(17156..18295)
                     /locus_tag="IWO89_000964"
     CDS             complement(17156..18295)
                     /locus_tag="IWO89_000964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HAQ0455736.1"
                     /translation="MQSRFKQSKLFFWTVELVLTIIGVYFILQMPNIFSPILKMLSAV
                     FLPLLVAGFIYYMFDPIVVFLEKHRIPRVFGFLLTFLVVIILVVLTVMNVVPQLMEQT
                     IQLTQSLPMYADETSKWLNELAQREEFKNFNLEEQLASANLSLSNLFNIILVSLSASV
                     SKIISFMMQFFILLFTVPFILLFMFKDGHKFIDALSHFFPKAIRKELRQTVKELNETL
                     SAYISSTILDALIIGVMSFLAMRIFDQPYSLLLAVICGITNIIPYVGPFIGAVPAIIV
                     GLFISPFQALYMALSILVIQQLDGNLIKPLLFGKSMNIHPLTIILVLIGAGSVAGILG
                     MLICIPVYAVIKTIILNIRKIYLLRRMEHVSEKLDSPKVLEETSE"
     gene            complement(18604..19317)
                     /locus_tag="IWO89_000965"
     CDS             complement(18604..19317)
                     /locus_tag="IWO89_000965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MgtC/SapB family protein"
                     /protein_id="HAQ0455737.1"
                     /translation="MNDMVLTIPEIILRLGIAMLFGGAIGFERQYKNRPAGMRTHILV
                     CMGACIIALIQVEIASGALRDALEYPQLAGTIRSDEARLIAQVVSGVGFLGAGTIIVT
                     KQSVTGLTTAASLWAIAGLGIAIGMGYYSIAIISFIGIAIALTVVKRVIHVPTTKKLE
                     IQFIHRKETKDFLTDYFEEKNIVIEDVNFDVKFVDDYRVYKNIYTIDLPKGLTYADVI
                     EELSVHKNITKLHLVSIAQ"
     gene            complement(19571..20272)
                     /gene="nagB"
                     /locus_tag="IWO89_000966"
     CDS             complement(19571..20272)
                     /gene="nagB"
                     /locus_tag="IWO89_000966"
                     /EC_number="3.5.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosamine-6-phosphate deaminase"
                     /protein_id="HAQ0455738.1"
                     /translation="MEIIRVKNAEEGGKKAFEIIKEGMNKGAKVLGLATGSTPVTLYK
                     EMTSSDLDFSEMTSVNLDEYVGLGGSDEQSYRHFMDVQLFDKKPFKETFVPDGKAEDL
                     EAACKKYDQIIDAHPIDIQILGIGQNGHIGFNEPGASFDGTTSVVDLTESTINANKRF
                     FDKAEDVPTKAVSMGIGSIMKSKEIVLMAFGESKADAIKGMIEGPVTNHLPASVLQNH
                     DNVVVIIDEEAASKL"
     gene            20506..22038
                     /locus_tag="IWO89_000967"
     CDS             20506..22038
                     /locus_tag="IWO89_000967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HAQ0455739.1"
                     /translation="MSILTLNNITQQFGDKILYEGVDLQLNAGEHLGLIGQNGAGKST
                     LIKIITGEILPDDGQIHWQKNIHKGYLDQYVEVDETLTIEEFLKTAYAKEYEKEEKIA
                     FLYQTYSETFDDSLLEKAGKLQTELDQGVFYQIDTLVAEMSSGLGIDVLGLDSLLKNL
                     SGGQRSKVILAKLLLENPDVLILDEPTNHLDDQHIQWLTQFLQDFDGAYIVISHDQEF
                     LDQITTHIADIEFGKITKYTGHLKQALKQKEQNRESYLRQYHAQQKQIEKTEAYIRKY
                     KAGSRSTMAKSRQKQLDKIERLTPPASASKAVFDFPFSPIVTTLAVETTDLVIGYDRP
                     LLAPINLTIRFGENIAIRGFNGIGKSTLLKTLIGEIEKISGDFHFPDNTKINYFSQDL
                     YWENPLETPLQYLSSQFPKATIKELRRQLAKAGLVNQLASEPLTTLSGGEQTKVKLAQ
                     LTMNQGNLLILDEPTNHIDQETKESLQDGIQKFPGTVIIVSHEQEFYQDLVQRVIEIE
                     PK"
     gene            complement(22229..23431)
                     /locus_tag="IWO89_000968"
     CDS             complement(22229..23431)
                     /locus_tag="IWO89_000968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAQ0455740.1"
                     /translation="MKSNEKKPLTTSLILLLSITCGVVVANMYYIQPIGTKVAASLSV
                     STSAVGILTMLTQLGYALGLLFLVPLGDVVDRPKLIIRMAALSAVSLLAAFFAPSFTL
                     FACASFLIGLLSIVPQIIIPYGAVLAGPKARGKVMGTLLSGLLVGILLSRTVSGIVAS
                     LFSWRMIYLFALCAVGILTLLLYWKMPRSVQQSSTAVSYLDSLKSLPHLVASQRLLRE
                     AAVSGFFMFGTFSIFWSTLIFYISSPAYNWGTFEAGILAIFGLSGAVAAPIVGRLADS
                     YSERKIVYMGLVMQTISFIALLVGGSHLAILLLAIILLDVGNQFGQVANQTRVQGLGE
                     AASNRNNTVFMFMYFIGGAAGSLLGTTMWQQYGWLGVTVSGLVFQGCAFFFQTVVFKG
                     KRNLENKK"
     gene            complement(23513..24202)
                     /locus_tag="IWO89_000969"
     CDS             complement(23513..24202)
                     /locus_tag="IWO89_000969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HAQ0455741.1"
                     /translation="MKKKIIRLILISLGIILILVVGYRLYLEYQTDIQLFLNPRASKA
                     ELLAHIRSHGTFASILLIVLTAVMCAVPGIPTSVVGVIAGLSFGPLTGAIINILGNAL
                     GNITAIFLMHRLKFLDKKTETNHWVQAIRQMKHPKIGVMLGYMIPVIPSSMVNFAADA
                     MKLPLQQIILSIVIGVIPSSVLYACGGEALFHGYNKTAVTLVASVTVLVGLVVFIYKD
                     RKKKQGKQKHK"
     gene            complement(24308..24676)
                     /gene="rplL"
                     /locus_tag="IWO89_000970"
     CDS             complement(24308..24676)
                     /gene="rplL"
                     /locus_tag="IWO89_000970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12"
                     /protein_id="HAQ0455742.1"
                     /translation="MALNIENIVAELKEATILELNDLVKAIEEEFGVSAAAPVAVAAA
                     GGAAAAEEQTEFTVELTAAGDQKVKVIKAVREATGLGLKEAKAVVDGAPAPVKEGVSK
                     EEAEELKAKLEEVGASVTVK"
     gene            complement(24729..25232)
                     /locus_tag="IWO89_000971"
     CDS             complement(24729..25232)
                     /locus_tag="IWO89_000971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L10"
                     /protein_id="HAQ0455743.1"
                     /translation="MSEAIIAKKAAIVDEVSEKFTAAASVVVVDYRGLTVDEVTRLRK
                     QLRDANVEMKVIKNSILSRAAKKAGLEGMDEVFTGPTAVAFSNEDVVAPAKIMDEFAK
                     EAKALEIKGGIIEGKVSSLEEITALAKLPNREGLLSMLLSVLQAPVRNVAYAVKAVAD
                     KEDGDAA"
     gene            complement(25642..26331)
                     /gene="rplA"
                     /locus_tag="IWO89_000972"
     CDS             complement(25642..26331)
                     /gene="rplA"
                     /locus_tag="IWO89_000972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L1"
                     /protein_id="HAQ0455744.1"
                     /translation="MAKKSKKMQDALKKVDSSKVYPVAEAVALAKETNIAKFDATVEV
                     AYRLNVDPKKADQQIRGAVVLPNGTGKTQTVLVFAKGDKAKEAEAAGADFVGDDDMVQ
                     KIQNGWFDFDVVVATPDMMATVGRLGRVLGPKGLMPNPKTGTVTMDVTKAVNEVKAGK
                     VTYRVDKAGNIHVPIGKVSFDDEKLVENFAAIHDVLLKAKPSAAKGQYMKNVTITTTF
                     GPGIHVDQASF"
     gene            complement(26435..26857)
                     /gene="rplK"
                     /locus_tag="IWO89_000973"
     CDS             complement(26435..26857)
                     /gene="rplK"
                     /locus_tag="IWO89_000973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11"
                     /protein_id="HAQ0455745.1"
                     /translation="MAKKVEKIVKLQIPAGKATPAPPVGPALGQAGINIMGFTKEFNA
                     RTADQAGLIIPVVISVYEDRSFTFVTKTPPAAVLLKKAAKIEKGSGEPNKNKVAKVSS
                     DQVREIAELKMEDLNAANVEAAMRMVEGTARSMGITVE"
     gene            27200..27868
                     /gene="sdaAB"
                     /locus_tag="IWO89_000974"
     CDS             27200..27868
                     /gene="sdaAB"
                     /locus_tag="IWO89_000974"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase, iron-sulfur-dependent,
                     subunit beta"
                     /protein_id="HAQ0455746.1"
                     /translation="MKDLRYRSVFDIIGPVMIGPSSSHTAGAARIGKIIRSIFGEQPD
                     SVDIYLYESFAKTYRGHGTDIALVGGLLGMEPDDERLADSLKLAYEAGMEVLFVPKSE
                     KAEHPNSVKMLLKKGTRKLSVTGISIGGGNIQISELNGFKISLSMGTPTLIVVHQDVP
                     GMIAKVTSILSETNTNIGTMTVTRESKGENAIMIIEVDDPQVEETVKRLKQLPNIDSV
                     NYFD"
     gene            27881..28753
                     /gene="sdaAA"
                     /locus_tag="IWO89_000975"
     CDS             27881..28753
                     /gene="sdaAA"
                     /locus_tag="IWO89_000975"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase, iron-sulfur-dependent,
                     subunit alpha"
                     /protein_id="HAQ0455747.1"
                     /translation="MFLSIEELVDQAKAYPSVAELMIAVEMEMSGRSRSHIIEIMEKN
                     LSVMEQSINEGKEGVTSVTGITGGDAKKLDDYLNEADFLSGETILKAVRNAVAVNEVN
                     AKMGLICATPTAGSAGVVPGVLMAVRDRLDLTHEQQLEFLFTAGAFGLVIANNASISG
                     AEGGCQAEVGSASAMAAAALVCAKNGTPHQAAQAVAITLKNMMGLICDPVAGLVEVPC
                     VKRNALGSSQAFISADMALAGIESVIPPDEVVAAMYQVGRQMPSIFKETAEGGLAVTP
                     TAKRLAEEILNKRA"
     gene            complement(28844..29863)
                     /locus_tag="IWO89_000976"
     CDS             complement(28844..29863)
                     /locus_tag="IWO89_000976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactate oxidase"
                     /protein_id="HAQ0455748.1"
                     /translation="MEAGAFGYIRGGSEDEWTMKENTTSFNTKKIMPRILRGIDSADL
                     HTSVFGIELDTPIIQAPSAAQGLAHEKGEADTAKGVAAAGSIFSISTYANTTIKDAAD
                     AAPGAPQFFQLYMSKDDRFNEFILNKAVEAGAKAIILTADSTLGGYREEDVINQFQFP
                     LPMPNLAAYSEQSASGNGEGKGIAEIYAAAKQGLTPDDIKTIKEITHLPVIVKGIQSP
                     EDAVIAISAGADGIWVSNHGGRQLDGGPASFEVLPKIAEVVNKRVPVIFDSGVRRGEH
                     VFKALASGADLVAIGRPVIYGLNLGGAEGVTSVFEHLNKELSITMQLAGTKTIDEVKN
                     TKLMD"
     gene            complement(30036..30923)
                     /locus_tag="IWO89_000977"
     CDS             complement(30036..30923)
                     /locus_tag="IWO89_000977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HAQ0455749.1"
                     /translation="MTEPIKNLCKRATAAALVLISLFALSGCQTTKINTTEVSDKEKT
                     AESSKVIEKTTIPTLFFHGYSGTENSFKGMLRRLEKNQQAVKELVLTVGTDGQIQATG
                     DLSGKADNPMVQVLFEDNKNNEWNQAEWIKTCLLYLKENYGIDKVNIVGHSMGGVSAL
                     RYLGTYGQDTSLPQVQTLTAIGAPFNDFVDDSAQSLTDELAKGPAVVSNRYQDYQQMI
                     GNIPITTRFFLIAGQLDETDLSDGTVPLNSALAVYALLKQRGNEIEEKVVTGENASHS
                     MLHENQEVDQLVSRFIWKE"
     gene            complement(31000..31545)
                     /gene="nusG"
                     /locus_tag="IWO89_000978"
     CDS             complement(31000..31545)
                     /gene="nusG"
                     /locus_tag="IWO89_000978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusG"
                     /protein_id="HAQ0455750.1"
                     /translation="MESFEKNWYVLHTYSGYENKVKANIESRAQSMKMADFIFRVVVP
                     EEKETEVKNGKEKEIVHKTFPGYVLVEMIMTDASWYVVRNTPGVTGFVGSHGAGSKPA
                     PLLPEEVNHILRSIGMSLRQNDLEVEVGEVVKIIEGAFAGLEGQVTEVDGEKEKLKVN
                     IDMFGRETSTELDFDQVDKID"
     gene            complement(31646..31816)
                     /gene="secE"
                     /locus_tag="IWO89_000979"
     CDS             complement(31646..31816)
                     /gene="secE"
                     /locus_tag="IWO89_000979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecE"
                     /protein_id="HAQ0455751.1"
                     /translation="MKFLKSVKDEIKQVTWPSRKQLRKDTLVVIETSIIFGLMFYVMD
                     TAIQSLFGWILK"
     gene            complement(31840..31992)
                     /gene="rpmG"
                     /locus_tag="IWO89_000980"
     CDS             complement(31840..31992)
                     /gene="rpmG"
                     /locus_tag="IWO89_000980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HAQ0455752.1"
                     /translation="MAKKKAALACSVCGSRNYTKSVSEGKSGERLEINKFCKYCNQYT
                     LHKETK"
     gene            32154..32798
                     /locus_tag="IWO89_000981"
     CDS             32154..32798
                     /locus_tag="IWO89_000981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HAQ0455753.1"
                     /translation="MLLKTLVYKKKDLTTVTETSTLEEALKILETSGYRCVPILDSSG
                     NIFRGNIYKMHIYRHKANGGDMTLPVTHLLKNATKFIYLDTSFFKVFFTIKELPYIAV
                     LDSENHFYGILTHSTLLNMLSQSWSVEQGSYVLTIASTGKQGDLAAIAKIISKHSSIA
                     SCITLDIEKDEYIRRTLITLPANTEAKVCETIVNHLEKKNYKVVEIENLQNQEA"
     gene            complement(32854..33357)
                     /locus_tag="IWO89_000982"
     CDS             complement(32854..33357)
                     /locus_tag="IWO89_000982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="QueT transporter family protein"
                     /protein_id="HAQ0455754.1"
                     /translation="MQKTKSRQRLSVIVVNGIIMALYLALTILVAPVSSGPIQFRISE
                     SLNHLVVFNRKLLWGVLGGVIVYNAFFGFGIMDVVFGGGQTLLSLGLTALLQNKVKNV
                     KMRLVLNTIFFTISMGLIAYMLVPTGGQAFWTTYGTLALSEFIVMAVSAPLMYYLDRA
                     LDFEKRV"
     regulatory      complement(33370..33416)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00522"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="PreQ1 riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="preQ1"
                     /db_xref="RFAM:RF00522"
     gene            33954..34919
                     /locus_tag="IWO89_000983"
     CDS             33954..34919
                     /locus_tag="IWO89_000983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family lipid kinase"
                     /protein_id="HAQ0455755.1"
                     /translation="MKFHYHILINPSAGSGNGYKVAERILPVLKNKHIDYTLHYSEYK
                     GHDAEIAETLAKETLIPWIEEEQKTIDTFPLLIIVGGDGTLHQVLDTFYQMEVEFPVA
                     YIPAGSGDDFARGAGLPKNPKKGLQLILAAQSPEKVHIMAYEEKISEKKGIAVNNFGI
                     GLDAAIVHATNHSSTKKRLNKYNLGSFSYLFSILRALFTQKGFPILVDAGGKQLSFSN
                     AFLCTATKHPFFGGGIAIVPTADASKPVIDFVVVERINFFKILWLILLLIQKKQLKSK
                     YFHHYTTNRLRIVSTTPQHGQEDGEEMEKRPFDITITTTKQLFWC"
     gene            complement(35018..36190)
                     /locus_tag="IWO89_000984"
     CDS             complement(35018..36190)
                     /locus_tag="IWO89_000984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAQ0455756.1"
                     /translation="MTLYKTIWTLLKANKSNLLIGVIITVVATFFFAQNIDVENTSLQ
                     DAKIALISEEESPVVDGLRKYLNDKETVVTLDGTSQKEIDDALYFNRVNIILHLPADF
                     TEQVEVGKMPTIKIQSRPDAFSKTVVTQQVNTFLQTLILFQEEDKTIDAAMEQTQMAL
                     SVTGEVELSAGYSQRMKKLLTGTTFNFLSYGLFLSIFSGFSVINLAFNRKEISKRNQS
                     APITKRNLNRKVTFSLISYSLLLFSGFLLLMVLLVIHTSWDITVWYHILNTFLFLLPI
                     ISFSACITSLVKNSETSGGIKDIFITGSCFIGGVFVPAEYLPEMVSKIAAFTPTYWFV
                     QNNNLISETLSYNQTFAESFWFNGCVLVAFAAVFSMIQFILGKERNYRWQPNWAKN"
     gene            complement(36177..>36302)
                     /locus_tag="IWO89_000985"
     CDS             complement(36177..>36302)
                     /locus_tag="IWO89_000985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAQ0455757.1"
                     /translation="KLYFYTDLASFYTNLLWLIGFIFLFALGNAWMERGVRYDAL"
BASE COUNT        11304 a         7222 c         6231 g        11545 t
ORIGIN      
        1 tatttccacc ttcttccaac aatgtctcgt aacgtaatgg ctgttgatta ataaattcat
       61 ttagttggac tatctcacaa acttctatta tttgttcgaa acttggtggg ctatctaaac
      121 cgaatataag attctcaatg attgttccac taaagaaaaa tgactcttga ggcaaatatg
      181 tcactcgatc gcgtaatatc tgaaaaggaa tgtctaagtg attaatgttt ccataatgga
      241 tcgatccttc tgatgcttca taaaaacgaa caaataactt tgcaagagtt gattttccag
      301 aaccactaac tccaactata gccgttttac tgtgcgaagg aattgtacat gaaatttcgt
      361 tcaacgttgg ggccttcatg ctatacgaga atgaaacgtt ttttacagtg atatcttgtt
      421 gaaataattg ttcagaaagc atatattcaa aagactggtt cgatttttct gaatcaatcg
      481 cgaaaatttc atttaaacga ttatttgcaa cttgagccgt ttgcattttc acttgtaaat
      541 taataatgtt ttgtaaagga tctgtcaaaa atactagtaa agcattgtat gtgataagtt
      601 gacctagact aattctatta tccataacaa aataagaacc tagccataaa attaatgcac
      661 tgctaattaa ttgaatcaaa tgtttaattc cttgttgaat attatcaagc gtaaccgtac
      721 gaaatgattt tttcattaat ttaacaaatt catggtctat acggttatat actttctctt
      781 caccgctata tgctttaatt gtttctattc ctttaagact ttctatgata ctagaattta
      841 cagctgcacc ggctgccatt tcttccctgt tcgatttttc atagcttttc acgaaagcga
      901 atattgcaac tacataaaaa ggcaaagagc atagagtgat taaaaacaac gtactattct
      961 gaatagctaa cgtaacccca acaagcaaca ccataccaat atctaaaaat attgataacg
     1021 tagcacttgc taaagcatca ataatcttac tggcatcaag aaaacgagaa ataatttccc
     1081 ctgattttct cgtagcaaaa aaattcattg gtaaagttaa cacatgtttg aaatactgaa
     1141 gcataattgc catactcatt cgctgtccta aaataataag aagataacta cgactatatt
     1201 caaacaatac ccgaaataaa tagacagcga tcaagccaat ggaaattata tttaaagttg
     1261 aacgtgcttg attagggatg aaaaaatcaa gaataccttg aaaataatat gagcttccta
     1321 tgccaaaaaa cgtaatgaaa atcgatgcta aaacgatgtg gaatactaat cctttttgtt
     1381 tccaaattat aggcaaaaaa gaacttaatc cagctacttt ttctttaata ggaatataag
     1441 tttctctagg cataggtaat aaaagaatac ctgtccattc tttttgaaac gcttcaatcg
     1501 tttttctatt ttttccatta gcgggatctg caatccatag tacgtttccc ttcgttttat
     1561 aaattacaac gtagtgcata tagctgttat caaccaatat atgtgcaatt aatggtaaga
     1621 taagatctct ttctttccag atctcactat ctgcttgaat agccatacaa tcaaaagaca
     1681 atttttcaaa tgtctttttc aatccaaatg ctgaagtacc ctctaaatca gtcccagata
     1741 ggtcccgtaa tttatgaatc gggatatcag ttttaaaata ttttaaaatc atcgaaaggc
     1801 aagcaacacc acaatctttt tcatcttgtt gctgcacaaa aggaaatgat tttatcactt
     1861 atctaacaca actccttcta aaaaattttt atagaaaaat tctcaatata gctttaatac
     1921 agtgaaaata ttgtctcata attccacaaa aactttgaaa acagttcttg aacactaact
     1981 ttttcattcc tgaatcaaat ttattttgga aattttacta ttcaagaata aacttgataa
     2041 gtttcaggaa gatttttact gttaatcaaa tacgtatgtt acagtagcac cgtaaaaaat
     2101 attatttaaa gaagcgtgtt taaacatgca aaatgtaaag gaactaagta ttgtggaaat
     2161 gcacaaactg ttggtggagg caataatgct tggggaaaat tggggcaagt tgtaggtgga
     2221 cttaccacag gggcagtagg tggtgcaggt ttaggaacag caatttgcgg accagcttgc
     2281 ggtgttgttg gagggttata tggagctgtc gctggaggtg ctgcagctgg ctgggatgct
     2341 cgaaaaaaat aaaaaaggag aataagtagt atgcatatta aaaacacaaa aacaacattt
     2401 atattatctt cagaagagtt gaaaaatatt caaggtggga gtgccgtggg agttttaggc
     2461 actacttttt ctggggcaac agcaggagta aaactttgtt cagccggagg accttatgca
     2521 atagctgctt gtggtgtagg tggagcttta ttaggtgctg gattcagcat gtggaccggt
     2581 gcgtaaacaa ttaaaaaata tcaaacctca agattggttt gtgtcgatta cgaacatagg
     2641 tatgttcttt atcttttcaa aagtttggaa tggacagaac tttttactaa agcttcttgt
     2701 gctagttttt ataattatat taaattttta tcttttatta aagtcagata aaaattcttt
     2761 ggaaatgtaa tggtagagat atttttacaa aaggacttgc agcgctgtag ttggtgctgc
     2821 aggaggacct atttcggccg ttacaggagg tattggtaat gtacttgtcg gatgtgcatt
     2881 aaagcctgtt cctgcaagtt aaatttataa aaacaggaaa gtaattatct caaatcctat
     2941 caagtacaat cataggattg cacttgacag gacaaatttt ttaactaaaa aatgaaaaac
     3001 actttctaaa aattagtaga atttgccaaa tctttcgcat cttccaccat acatttaatt
     3061 ttaattcccc tttatggtac ttaataaaaa caagaaaaga actattcatt tggccctttt
     3121 ttggttccag accatagaaa tattcctcca gtaactaata ttgctgttat gcacgtaact
     3181 aataacagct ataaaaattg gtacaacttt aataggattc agaaaaattt gcactagtga
     3241 aggaatctat tttattagaa aaatgcaatc tccctaaaga aaaaaccact aaacagtgct
     3301 ttctttcaac ttctccttaa ttttttgtgc tttccctaaa gtaaagtttc tggccaactc
     3361 tgaatcaaaa tatagtgaac gttttttaaa atctatttct ttaacgttaa agatatttac
     3421 taaacacgct cgactaattc ttgttaacat aggatatttc ttttcaagct cactaattct
     3481 tccataaaat tcgtactgcc catctttagt acacaatgat aatctatgtg gtaattttga
     3541 agcttctaca aaataaatgt catttatatc aaaagtaaat gtttgagaac ctatggaaaa
     3601 aacaaatgct tgattcgtaa cctgttttgt tgcatctatc ctttcttgcg ctaataacaa
     3661 taactcaaca atctcgtttc gatactcatc tagtgtttga tcttttgtta caaatcctaa
     3721 cgtttcaact cttcttttta ttgttacggg taacatctca tcatgagtag ttgtaaaaat
     3781 gatttttgct tgaacatcat attttctgat tacttctgct aattctatac cgttaacttc
     3841 atgatttaaa tcaatatcta agaaatatat tccttgcttt ggtcggaatt gtttgaggta
     3901 ttttttaact tctaacggac tttgtgtttt caatactatc ttaaatactt tatcgtgaaa
     3961 aagaatgaaa ttatcaataa ttcgttctaa ctgatttaat tgtataattt gatcttcaca
     4021 taatattatt ggataactca taaatcctcc ttccctcaaa acaacaaaat aacttgagta
     4081 gtaaattgat acggatcgtt ttgatagttc acaaacatat tgggattctt tttattgatt
     4141 tcctctatac tagataaacc taacccttga tgtccctttt ttgttgttat tccttttttc
     4201 attagagtat ctatagagag tagtatctct ctacaactat tcgatatcga aatttctatc
     4261 tgtttatcat ctcggtaaat agctagagac agttctctat cttgactctc tatagatgct
     4321 tcaattgcat tatcgagaat aattccaagc accctaatac agtcaaatat tggaacagga
     4381 acttcaataa tagtacttgg acattcgaat ttacaacaaa tcttattgtt atttgcttgg
     4441 tatagcttcg ctatcaacaa acttttcaaa tattcatttt ttatattttt taaataacga
     4501 tattcaaact tattggttag ataagaatta gaatagatct ctaattcttt tatttctttt
     4561 tccaatatcg aatcatggtt ttgattagcg atttctttta aactcagcaa taaattttta
     4621 tagtcatgtc taaatctggc aaatttttct tgatcttttt ccagtgaatc agtatatttt
     4681 tttagataaa ccaattcttt tcttttcaat tgctgttcat attgttctcg ttgcctaata
     4741 gttattctaa taaataaaaa tagaacaaca aacatttgaa tgattaaaaa tattaagaca
     4801 ccaaacacaa aatgatcaaa tacttgatat tgatgagcaa cataagaaac aaataaacca
     4861 attgtaagta aataaatcat gaatatagca gtaagtttag aactggactg ttcaacaatc
     4921 ctatttatag ctaattttct atataaccaa ataaacatca gcaaaaacaa agaatcaagc
     4981 attatctgga atactacata accataccct tttatccctt caaatgaaaa catatacgtc
     5041 ataaccatgg aagtaataat agatctgaaa tcatcaataa gcatacataa tagtaattta
     5101 tttaaaagca ctatattagt tttcttcttt tcctttaata taaaataact tccgattata
     5161 ggtatagctg cggaaacatc agtaaataat tcaacaaaaa aagtgagtgg tatcaataaa
     5221 catacaaaaa gatattttct tttgttaaga tcagtattag tcatataaaa taagagaaaa
     5281 aagaaaaagt ttcccacgaa cattcttaat agataagctt ttgtatcgat agtaatcaag
     5341 agatcccctc ctgattttag gatatcataa aaaaaatttt agttacactt tttaaataat
     5401 gaaaaaacac attccactaa atttgatgcc ggttttcctt gaggttttgt tccagcacct
     5461 cctttaattt ttttaagctc ttgatctgaa cattgtttag catttagtct atttttttct
     5521 tccattacga ttccccctac aaactataaa tacttttatg tcactgattc aatataccta
     5581 tttttctaat caaaaaattt tgagacatat ttaatattat aagtatcatt aaaattgaga
     5641 tttatctcca taatctgctc gatataaacc atttattcca gcagttcttc caatttcact
     5701 caaatttctt attaattcct cttgatattc agtgaatctt attttattag tataagcagt
     5761 aaaatataga taattcacca atctatttat taaaggaccg ggatctaatt catatttttg
     5821 tttttctaat tttaaatata ccttttgtaa aacctcaaga atatcagaat atttaggatc
     5881 tttacttata gatatatcat tatataattc atgaacaatt tgcttagcat tttttttcat
     5941 tttagcactt ccctggaatt gctccaccta aaaaaccacc tatagacatt cctgcaatac
     6001 aagtagttgc cttggcccaa tcgaccgtac atttattttt agtgcaatac actccatttc
     6061 cataatattt tccactatga gtggtacccc catagataag ttgtgtctct ttaatagaca
     6121 aaatttttaa atgtttcata atctaatcac ccctaaatta atgtttttag actattattc
     6181 taacattcat taaatttcaa aaaacaattc cttcttgaaa cgtagcaaaa taattcctga
     6241 ataagaattt aatcactttt aagtatataa actatgtata ttctttaata ttttttgaga
     6301 atgcacttcg tacataataa actttaatca cattagtctt tcatacctac gatcgatctc
     6361 tttgactcgc acccataaat tcataatagt ttgtcggcgt ctttggacgt ttcgaagatg
     6421 agctacgcat tctctttttt tatagacaaa aaagatagag acgaacatgg agcttccaaa
     6481 tttggatatt tgatccaatt ttggaagctc catttatttc gtgtctgtct ttttattttt
     6541 tccagctttc gactaattct tggttctctt tgatccagtc ttttgctgct tgctgtggat
     6601 cttttccatt gttgatttcc aacataactt tttccatatc tttttctgac caatggaaat
     6661 tgtctaatac tttgtaagct tctggctggt cttctttcaa tccttttcga accatcgtat
     6721 ggatctgctc ttcgcttccc attccgtttt caggatcttc taagtatttc agatcatatt
     6781 tggcaaacat ccaatgaggt gtccatcctg taactacaat tggttcgtgt tttttgatcg
     6841 cttgacctag tgcaacggtc attgctcctg aagaagatgt ctctactttc cagtctttca
     6901 agttgtcata tttttggatc gtgttttcag cagccgtaac tacaccagct cctggctcga
     6961 ttcctatgat tttcttacct gcctgatcgg ttaactcatc gatcgaattt acatccatgt
     7021 aggctggcac tgctaaacca acttttgctc ctgtcaaatt ggcacctagg tcttcgactt
     7081 gatctttata ttgtgcatat tgtgaaccat gagttttagg tagccaagct gaaaccatgg
     7141 catctgactc gccatttgaa acagatttcc acatgattga attatctaat ggcgtcatcg
     7201 ttacatcata gcccatttgt ttcaatactt caccgactac atttgttgaa gcaacttctg
     7261 tatcccattc aacataagat aagttgatac gttttgtttc tttggcagat gaaaaactcc
     7321 ctataccaat cgcacctaag atcactacta ctacagcacc aatgatcagt ccttgacgtt
     7381 ttttactttt ctgtgttgtt gctcctgcag cttttttatt gtttggctgg ttcaaatgct
     7441 gtgtaaaacg gtcaatgatg attgctaaga taactaatgc aaccccgttg acaaatccat
     7501 tcccgacttg tgctctttgc aatgctgata atacgccgcg tcctaagccg ggtgcaccga
     7561 tcatagaggc aataacgacc attgaaagtg acagcattgt tgtttgattg atccctgcca
     7621 tgatcgtact tttagctaaa ggcaattcta gtttgaatag tttttgacga cttgtgctcc
     7681 cgaatgaatc agaagcttct actaattctt taggaacttg tcgaatccct aaattcgtaa
     7741 aacgaacggt tggtggcaga gcaaagatga ctgaggcaaa tacccctgga accatcccga
     7801 taccgaaaaa tgcaactgct ggaatcaaat aaacgaatcc tggcattgtc tgcataaagt
     7861 caaggatcgg tttgataata ctttgggctt tactgctttt tgccatcaag atccctaatg
     7921 gtaccccaat gatgatcgag atcacactag ataaaagaac aagtgtgaca gtattcatca
     7981 aatcattcca tagaccttgg ttataaataa accatagacc gattaatgtg aacaacgtaa
     8041 gtcctggacg tttcttagaa ataaagaaag ctgctaccgt caataacaag ataaacaaag
     8101 gagctggaat cactaacagt aaatttgtaa tccccgacat cactgcctgt ccaatcgttt
     8161 gtaaaaatga aaataggcca gaaaaagttg tcgtaaacca ttctgtgata ttttctaccc
     8221 agtttgccac tggtaattga tagttcaata gattattcat gttcactcac ctcgcttgtt
     8281 tctgctaatg cttcaatcac actacctcgg atgacgacac cttttaattt tccatcctct
     8341 acaactgcaa gtggagtcgg tgagtcataa ataatattga aaatatcact taccaccatg
     8401 tctttcccaa tcaccgtcac attttcatct aggaagtcga tcaatggacg tttttgcttc
     8461 ctagcttcta acgcttgatc tgcagtgatg atccctttca ggtgtctctt tctgtcaaca
     8521 gccatcagca tgctgacttc ttcattacgc atccttgtca atgcaacgtt tggtccatca
     8581 gattcgatat tcgtcgtcaa tgcaggtacc atgatgtttt gagccgtaag cactttggaa
     8641 cgatcgactt cttccacaaa ttcacgtaca taatcatttg ctggattggt caatatttct
     8701 tcacctgttc cgatctgcat gatttcgccg tctttcatca aggcgattct gtccccaatc
     8761 cgtaaagctt cattcaaatc atgggtaatg aaaataatcg ttttttgaac attggcttgc
     8821 aggtccagta attcgtcttg catttctcgc cggatcagcg gatcaagagc cgagaacgct
     8881 tcgtccatca gcaagatctc aggatcattt gctaaagctc tggctaggcc aacccgttgt
     8941 tgcatcccac cagataactg actaggatat tggtctttaa atgaaagcaa actagaattt
     9001 tctaatgctt tttctgcttt ttcttgtcgt tcttctttag gtacaccacg gacttctaac
     9061 ccgtattctg tattttccaa gatcgttcga tgtggaaaca gaccgaagtt ttggaaaacc
     9121 atattgattt tatgacgacg gacttctcgc aattcttctt tgcttaactt cgatacatct
     9181 tctccatcaa tatagatact gcccgaagtt ggctcaatca gacgattcaa tagacggatc
     9241 aatgtggatt tcccactccc ggaaagcccc atgatcacga agatttctcc ttcttttacg
     9301 tcaaaattca cgtcatacac accaaccgtt gcaccggttt tctttaaaat ctccgttttc
     9361 ggctggtgat cattcatcat atctaaagcg gcttgtgtct ttttaccaaa tatctttgtt
     9421 aaatgtttta cttctacttt taccaaacaa attctccttt tttatgtaaa ttcatttttc
     9481 tttctgaaaa aagtgaccac actatgttaa catcatgtag tcactttgtc aaaaatgaaa
     9541 gaaatttgct tgtccaaact gaaaaaaact tggctaaatc tttgtaaata aaaggtttag
     9601 ccaagttaat atttttttaa tatttcacac aatcattctg caagctgatg ctccgggtaa
     9661 aaaaagtttg tcatacgctg tagtgtatat tcatttccaa taaaatgaac gatgtcccct
     9721 gccgtaaatt tagcataggg gccaggtgaa acaagcagtt cttcattatg agaaattgcg
     9781 ataactgtgg cgccagtcgc ctgccagata ttcaactcgc taattgaacg gtccagatgc
     9841 attgcttttt ctgataaaga taattcaaac ggaagtaaag gattcatctt atgtattcgc
     9901 ttcgtttgat tgaccagctg atccaataat tgagaaaaat gattcagctc atcttgttgc
     9961 ttctccacac ttgccatgat ttctgctttt gtatcttgga tggattgtac atcttgatat
    10021 tgcaatagga aatcttttgc tttttccttc gaagcaacaa atgccccact tccatgccgt
    10081 acttccatga ttccaagatc taccagcaca ttgatagcct tgcgggtagt ctcaggagaa
    10141 gcattgtacg tactggcaag tgttgagcgg gcatggatct tctcacccac tttatatcgg
    10201 ttttcaacga tacgttcggc taaatcaaca gcaatctgct ggtagcgcgg gcggacgatt
    10261 tttttctttc cagacatttt ctttcctctt tcttttttta ctttccctca tcttactatg
    10321 ctctagcaaa ataagcaaaa aaggggagaa tagcctcttg caacgactga tcatcgcttg
    10381 atctgttcct tttttactgg gcaagctgtt tgacagtcat cacaattctt accggatttt
    10441 aaacgagtat agacaattgc gcctgcactt ccaaagatta acacacttaa taagaatgtc
    10501 gccatcctat tcctctcctt ctaatgacaa tatagtataa aactggttct tggattttgg
    10561 ctgacggaac aaaacataaa cgagtcctat catcagtaaa atagcaagat acgtccctaa
    10621 cgctacctgt ccaccctcca ataagtgtcc caactgataa atcacaaaac taactaaata
    10681 agctaaaccg cattgatagc ctatggcgat ccaagtccat ttcttggcat tcatttcccg
    10741 atggatagcc ccaattgccg caaaacaagg cgcacaaagt aaattgaagg ctaacaatga
    10801 ataagctgct gcgggagtat atgtgtgttg tagcacttgc cagatttctc tgccattttc
    10861 ggatacttcc gcatgaccaa acaaaatacc aaatgtgcca ataatatttt ctttagcgac
    10921 caatcctgta atcgctgcga ctgttccttg ccagtttccc caaccaagcg gctgaaaaac
    10981 aactgccagg attcgcccga aaaaggctaa gatactttgg tcttcagaaa caggttgagc
    11041 ataaaaatta taagaagagc tgaaccagat cacgatacta gaaacaaaga taatcgttcc
    11101 tgctttttta acaaaagact tgctgcggtc ataggtttgt cttaggaccg ctgaccattg
    11161 aggaaaatga tatgcgggca attccatgat aaaaggagct gtatctccag caaacaaacg
    11221 tgtttttttc aagctgattc cagagaaaat aattgcagtc ataccaagaa aataggcaga
    11281 aggagctacc caagattgat gaggaaaaaa ggcccctgct actaaggcaa taatcggcaa
    11341 ctttgcagaa caaggcatga aggttgtgac catgatcgtc aaacgacgat cttgctcgtt
    11401 ttcaatcgtg cgactggcca tgacgcctgg cactccacat cctgtagcaa tcagcatagg
    11461 aatgaaggat tttcctgaaa gaccgaattt cctgaaaagt cgatccatga caaaagcgat
    11521 cctcgccata tatccgcagt cttccagaaa gctcaagcat aaaaacaata taatcaattg
    11581 cggtagaaat cctaacactg ctcccacgcc tgcgatgatg ccgttcaaga tcagctcctg
    11641 catccatccc gctacttgcc attcagctaa caatctgcca acatgttctg gtaaccatgt
    11701 accaaacaat gtgtcattga tccaatctgt ccccattgtt cctactgttt gaatagccag
    11761 ataatagatc agccacatca caaatgcgaa gatcggcaag gctaaccaac gattcgttgc
    11821 aatctgatcg attttgtcac tcatcgataa ttggaaagaa tcatttgaca caacacagag
    11881 tgtacatagt tgggtaataa attcataacg ttcattgacc agaatcgtct cactatcgtc
    11941 gtccaatagt ttctctgtta actggatcag ttgctcgatc tctttttgct gagaaactgc
    12001 tatctcgtaa tcctctctta ccttctgatc tcgttcaaaa tatttcattg cataccagcg
    12061 tttcttgccg ctttgttctc ctagtacatc ttcaatttca tgtaaagctg cttctaaacg
    12121 tggatcatat gtacagggaa caggaataga aggccgcttt tttgcagact cctgaatctt
    12181 ttcgattagt ttttcgattc cttgcttttt caatacgctg attggtaaaa ctggtacacc
    12241 aagaccatag gaaagctttt ctagattgat tttttttcct tcttttttga tgacatccat
    12301 catattcaga ccaatgatca taggaatgtc taaatccaat aactgcgtag ttaaataaag
    12361 atttcgttct aaattggtag catcaataat gttgatgatc agttcaggct gttcctgaac
    12421 caaataatct cttgcaatca cttcttctgg tgtataagga gacaaagaat agatgccggg
    12481 taggtcctga atatgaatgt tcggattttt tttcaacgat ccttcttttt tttctactgt
    12541 tacgcccggc caattcccta catattgatt cgaaccagtc aagagattga acaagctagt
    12601 tttcccgctg tttggatttc cgactaacgc gatattcatt ctttttcctc cctttgtatc
    12661 aagattagtt cggcctctga ctttcgtaac gtcagttcat accctcgcac tgtcagttcg
    12721 attggatctc ccagaggggc tagttttcgg ataactattt ttacatttcg cgtcaatccc
    12781 atatccatta accgtcgctt cactgcgcct tgtccgtgga ttgctacgac gatcccagtc
    12841 tctcctgctt ttaattgatc aagagaagtc cagctttcag aggaagcagt ctttttattg
    12901 acaaagattt gacctagtat ttcccgactt actgccactc gatgactttt taccagtaaa
    12961 atggcattgt tgcctgataa attgacgacg acgattttac ttccggcaac gatcccgata
    13021 ttttgcaaat ggcgttttgt tttttcttct gtttcgattc gttcgacttg ataaacgaaa
    13081 ttctgctttg cctgcgctaa ggtctgcata ttgattcctc tcttgtgaaa taaatcatgt
    13141 attaaaatca ttcattatat gttctaattt tcttacgtgt tacgcaaata aatgagaatc
    13201 attctcacat agaatactct cggtaattga gaatgtcaat tatacttgtg atattatgag
    13261 cgaatcttta gtattgatag ataaaaatga gaaaaaaata tttcttcata gttttccaca
    13321 attttttctc tattaaattt gctattttcc cacacgatac ttgttttcag ctcttttttc
    13381 aaaaacacaa aacttagtat tccacagttt ccacaacaca atatgtagtg tttgttgttt
    13441 gactttttta ctaagagttt tatactatct attatagata ataagaatgt gttagaggag
    13501 gcactcacca tgaacgtaaa aatcttttct aaaaataatt gtatgcaatg taagatggtc
    13561 aaacggtttt taacagaaaa tcaaattgct tttgaagaag tcaatatcga tgaacagcct
    13621 gaagcagtag actggttgaa agcacaagga ttccaaagtg ttcctgtcat tacttctgat
    13681 gcagaaacag ttatcggatt ccgtcctgat cagttgcgta aacttgccag ctaacatgac
    13741 taaaagaatc aaaacgtgat acatggattt gaagggcttg agacagcacc agtttttgtc
    13801 gcaaacttcc aaatcctttt ttaaagctta aaaacaatgc aatgatttgc aatcaagcat
    13861 catcaaatac gaaatgaagg ttgggcgtac gtatgagtct taaaaattta caagatgtca
    13921 gttatttcaa gctgaataat gaaatcaatc ggccagtcaa tggtcaaatc cctttgaata
    13981 aggataaaga agctttagtt gccttttttg aggaaaatgt caaaccaaat acgatgactt
    14041 ttctatctat tatggaaaaa atcaattact tgatcgatca agaatatata gaagcagaat
    14101 tcattcaatt gtattcacca tcatttattg aaaagctata tcaatttttg gacgagcaac
    14161 atttttcttt caaatctttt atggctgctt acaaatttta ttctcaatat gcgttgaaaa
    14221 ccaacgacgg ttccgcttat ctggaaactt atgaagatcg agtagccttc aatgcgcttt
    14281 actttgcgaa cggggatgaa gaattagcac ttgctcttgc agacgaaatg atccatcaac
    14341 gatatcagcc agctacacct tctttcttga atgctggacg taaacgtcgg ggcgaattgg
    14401 tgtcttgttt cttgactcaa gtcactgacg atatgaactc tatcgggcgc tcaatcaact
    14461 ctgctttgca attgtcccgt attggtggcg gcgtagggat tactttatca aatcttcgtg
    14521 aagccggcgc tcctatcaaa ggatatgagg gtgctgcaag tggtgtcgtt ccagtcatga
    14581 aattattcga agatagtttc agctattcta atcaattagg tcagcgtcaa ggagctggcg
    14641 tggtttattt gaacgttttc catccagaca ttattgcttt cctttcagca aaaaaagaaa
    14701 atgcagatga aaagattcgc gtaaaaactc tttctcttgg cgtgatcgta cctgataaat
    14761 tttatgaatt gacacgtaaa aacgaagata tgtatttatt tagtccatat agtgtcgaaa
    14821 aagaatatgg tgtcccattc tcttatgtaa atattacagc tgagtatgat catctagttg
    14881 caaatccaaa cattcgcaaa caaaaaatca aagcgcgtga tcttgaaaat gaaatctcaa
    14941 aattacaaca agaatctggc tatccttacg taatcaatat tgatacagcg aaccgcgaga
    15001 atccaatcga tggcaagatc atcatgagta atttatgttc agagatttta caagttcaaa
    15061 cgccttctgt gattaacggc aaacaggaat atgaagtatt aggaacggat atctcatgta
    15121 acttaggctc gacaaatatc gtgaatttga tgcaaagccc taactttggt caatctgtgc
    15181 gtacgatgac tcgtgcattg acatttatta cagatgcatc tgatatcgat gtcgtaccac
    15241 cgatccaaaa cggaaataag ctgaatcata cgattggatt aggtgccatg ggcttacaca
    15301 cttatcttgc aaaagaacaa atggaatatg gctcagagga ttcattagac tttacaaata
    15361 tttacttcat gctgttgaat tattggacac ttgttgaaag caacaatatc gctcgtgaaa
    15421 gaaaacaagt tttccataat tttgaaaaat cagcttatgc tgacggcact tatttcgaaa
    15481 aatacgtaac tggagaattc cagccaaaat cagaaaaagt caaagcattg tttgaaggca
    15541 tttttatccc aactgctgaa gattggaatg ctttgaaaca agcagtaatg aaagatggac
    15601 tgtatcatca aaatcgcttg gcagtagcac caaatggctc aatttcatat atcaacgata
    15661 cgagtgcaag tattcatcct atcactcgca tgatcgaaga acgccaagag aagaaaatcg
    15721 gtaaaattta ttacccagcg ccttatctat ccaatgagac gattccgtat tacacttctg
    15781 cttatgatat ggacatgcgt aaagtaatcg atgtttatgc aatggcacaa caacacgtcg
    15841 atcaaggcat gagtttgact ttattcatgc gttctgaaat cccagaaggc atttatgaat
    15901 ggaagacaac aacgaagcaa acgacacgtg atttgaatat tttaagacat tacgctttcc
    15961 acaaaggaat caaatccatt tattacgtac gtacctttac tgatgatgca gaagaaatcg
    16021 gaagcaatca atgtgaaagc tgcgtgatct aatatcatag atagattcgc ttattcatat
    16081 tttaattaaa ggagcattct catgtcagaa acatattatg aagcaataaa ctggaatgaa
    16141 attgaagata tcattgataa atccacttgg gagaagctga cagagcaatt ttggctagat
    16201 acacgtatcc cactttcaaa tgacctagat gattggcgta cattgtctca attggaaaaa
    16261 gatacagtag gacatgtatt tggcggtttg acactgctag atactgtcca atctgaaagt
    16321 ggcatggatc agttgcgtaa agatgtccgc actccccatg aagaagccgt tttgaacaat
    16381 attcaattca tggaatccgt tcatgccaaa agttattctt ctatttttag tacactgaat
    16441 acgaaaaaag aaatcgaaga aatttttgac tggacaaata cgaatcctta tctgcaaaaa
    16501 aaagctgagc gtatcaatga aatctacaaa cacggtacac cgctagaaaa gaaaattgcc
    16561 agcgtctttt tagaaacatt cttgttctat tcaggttttt atactcctct ttattattta
    16621 ggaaacaaca aattggctaa cgtagcagag atcatcaaac taatcatccg agatgaatct
    16681 gtacatggaa cctacatcgg ttacaaattc caattaggat tcaacgagtt acctgaagca
    16741 gaacaagaaa cgttaaaaga ctggatgtac aatcttcttt atgagcttta cgaaaacgaa
    16801 gaacgttata cagaagaatt atacgatcca atcggctgga cagaagaagt caagacattc
    16861 ctacgctata atgccaacaa agcattgatg aacctaggga tggatccact ctttccagat
    16921 actgccaatg atgtcaaccc aatcgtaatg aacgggattt caacaggcac aagcaaccat
    16981 gacttcttct cacaagtcgg aaacggctat ttgttaggtc atgtagaagc gatgaaagac
    17041 gacgattatt tgattggatt ggaataagga tttattttaa ttcaaaaatt tttcgaaaaa
    17101 taaaaagagt ttagcgtaca gatattttaa atgtacactg aactcttttt tatgcttatt
    17161 cacttgtttc ttccagcact tttggactgt ctaacttttc cgaaacatgt tccattcttc
    17221 tcaataaata gattttccgg atatttaata ttatcgtctt aatcactgca tagacaggta
    17281 tgcaaatcag catgcctaat attcctgcga cacttcctgc tccaatcaat accaagatga
    17341 tcgtcaaagg atggatattc attgatttcc caaataacaa cggcttgatc aaatttccat
    17401 ctaattgttg gatcactaaa atcgacaacg ccatatacaa agcttgaaat ggagagataa
    17461 acaaaccgac aatgattgct ggtaccgctc caataaaagg gccgacatac gggataatat
    17521 tcgtgattcc gcaaatgacc gcaagcaaca agctataagg ctgatcgaat attctcattg
    17581 ccaaaaagct cattacacca ataatcaaag catctaagat cgtactgctg atataggcag
    17641 ataatgtttc gtttaattct tttaccgttt ggcgtaactc tttacgaatc gctttcggaa
    17701 agaagtgaga caacgcatca ataaatttgt gcccatcctt gaacataaat agcaaaataa
    17761 atggaacggt aaataacaaa ataaaaaatt gcatcataaa tgaaatgatt tttgagacac
    17821 ttgcagataa actgactaat atgatattga acaaattact aagagataaa tttgctgatg
    17881 ctaattgttc ttccaaattg aaatttttaa actcttcccg ctgcgccaat tcatttaacc
    17941 atttgcttgt ttcatctgca tacattggca agctctgggt caattgaatt gtctgttcca
    18001 ttaactgagg cactacattc atgacagtta aaacaactaa aatgattacg accaaaaacg
    18061 tcaataaaaa accgaacact ctcgggatcc gatgcttttc gagaaaaaca acgattggat
    18121 cgaacatata atagataaaa ccagcaacaa gcaatggtaa aaaaactgcc gaaagcattt
    18181 tcaatattgg actaaaaata ttcggcattt gtaaaataaa gtacacgccg atgatcgtga
    18241 gtactagctc cactgtccag aaaaataatt ttgattgttt aaaacgtgat tgcatacatc
    18301 tcctccaagg tcctttgata cttctattct atcatttaac aaagagttac gcgctgagat
    18361 tttcatttgc ttaaaaaaaa caaaaaaaat aaacatattt taatgagtag acgagtactt
    18421 aaacaaaaac acggtttgga gtagtgacaa aaattatact gcagtccctt tttccaaata
    18481 aacggtgttc aaaagcttgc atcttcgtaa taagcccagc caaacgaaaa atataaggaa
    18541 ctatttttat ttggctgggc ttattacttg atgtaggaca tctattttac aacctcagat
    18601 catttattgc gcaatactga ctaaatgcaa ctttgtgata tttttgtgta cggacaactc
    18661 ctcgatcaca tcggcataag tgagcccttt tggaagatca atcgtataga tatttttgta
    18721 cactcggtag tcatcaacga attttacgtc aaaattcaca tcttcgatta cgatattttt
    18781 ctcttcaaaa taatcggtaa gaaaatcttt ggtttctttt ctatggataa attggatttc
    18841 taacttttta gtcgtaggta catggatcac gcgtttcact actgtgagtg caatagcaat
    18901 cccgataaaa ctgatgatcg caatggaata gtaacccatg ccgatggcga tccctaatcc
    18961 agcaattgcc cataaagaag cggcagtcgt aagtcccgtc accgattgtt tcgtgacgat
    19021 gatcgttccc gcgcctaaga aaccgacacc actcaccact tgagcaatca gccttgcttc
    19081 atctgaacga atcgttccag caagctgtgg atactctaaa gcatctctta atgcaccact
    19141 agcgatctca acttggatca atgcaatgat acatgctccc atacaaacta aaatatgcgt
    19201 acgcatacct gctggacgat ttttatattg tcgttcaaaa ccgattgctc cgccaaatag
    19261 catagcgatc cctaaacgta aaataatctc tggaattgtc aataccatat cattcatcta
    19321 aacaccctcc agtccaatat atcatgtctg aaaggagcag acaaaaaaag tgcaattttt
    19381 acctcttacg aaaaaaggac tgtgacaaaa tttttgtcat agtccttttt atgaataaac
    19441 ggtgttcaat cagctgaccc tcggtattac tcaacaaacg gcaaaatatg gggagctatt
    19501 tttcctttgt cttctctaat actcaggtca aaacgctgat ttcacaacct ctttagccta
    19561 ctattgaaaa ttacaactta cttgctgctt cttcatcgat gatcacaaca acattgtcgt
    19621 gattttgcaa tacacttgct ggcaaatgat tcgttacagg tccttcaatc atccctttga
    19681 ttgcatcagc tttgctttcg ccaaatgcca tcaagacgat ttctttactt ttcataattg
    19741 aaccgattcc cattgaaaca gcttttgttg gtacatcttc tgctttatcg aagaatcgtt
    19801 tgtttgcatt gattgttgat tctgttaagt caactactga agtcgttccg tcaaatgaag
    19861 caccaggttc attgaaaccg atatgtccat tttgcccgat tcctaagatt tggatatcga
    19921 ttggatgtgc atcgatgatt tgatcatact ttttgcatgc tgcttccaga tcttctgctt
    19981 ttccatctgg tacaaacgtt tctttgaatg gttttttatc aaataattgg acatccatga
    20041 aatgacggta gctttgttca tctgaaccgc ctagtccaac gtactcatct aagtttacag
    20101 aagtcatttc actgaaatcc aaatcgcttg aagtcatttc tttatataaa gtaactggtg
    20161 tgcttcctgt tgccaatcct aatacttttg cacctttgtt cataccttct ttgatgattt
    20221 caaatgcttt ttttccgcct tcttcagcgt tttttacacg aatgatttcc atagtgattg
    20281 ctcctttata ttttggtata gcccaattat agcattcttt ttttcttttt acaataatta
    20341 gtatagacca aattgcattt ttttcatttt cttgacaata aataggaatc cgtgttaggt
    20401 ttcataagat ggttcaatca aacaatccat gttgcatgaa aaccaagcaa gtgattcact
    20461 gtggacgctt tatgtctgca gtttttttat gaaaaaggag ttattatgag tatactgaca
    20521 ttaaacaaca ttacccagca atttggcgac aaaatcctct atgaaggtgt cgatctgcaa
    20581 ttaaatgctg gcgaacatct tgggctgatt ggacaaaacg gagccggtaa aagtacattg
    20641 attaaaatca tcactggtga gatcttacca gatgacggac agatccattg gcaaaaaaat
    20701 attcacaaag gttacctcga tcaatatgtg gaagtcgatg aaacattaac aatcgaagaa
    20761 tttctaaaaa ctgcgtacgc aaaagaatac gaaaaggaag aaaaaattgc ttttctttac
    20821 caaacatata gcgagacctt cgatgattcc ttgctggaaa aagctgggaa actccaaacg
    20881 gaattagacc aaggagtgtt ttatcaaatc gacaccttag ttgctgagat gagcagcggg
    20941 ttggggatcg atgtcttagg tttagattca ctgttgaaaa atctaagtgg tggacaacgt
    21001 tcaaaggtga ttttagcaaa actactttta gaaaacccag atgttctgat cttagatgaa
    21061 ccaaccaatc acttagatga tcagcatatc caatggctga cgcagtttct tcaggatttt
    21121 gacggtgcgt atatcgtgat ttcccatgac caagaatttc ttgatcagat caccacccat
    21181 atcgcagaca tcgagtttgg aaagattact aagtatactg gccatctaaa acaagcattg
    21241 aaacaaaagg aacaaaatcg cgaaagctat ttgcgccaat atcatgctca acaaaaacaa
    21301 atcgaaaaga ccgaagctta tattcgaaaa tacaaagccg gttcccgatc aacgatggca
    21361 aaaagccgac aaaaacaatt agataaaatc gaacggctga cgcctccagc ttcagcaagt
    21421 aaagctgtct ttgattttcc attctcgcct attgtcacga cattagcagt ggaaacaact
    21481 gatttagtca tcggatatga tcgaccgcta cttgcaccca tcaatttgac catccgcttt
    21541 ggtgaaaata ttgccattcg aggcttcaac gggattggaa aatctacttt attaaaaaca
    21601 ctgatcggtg agattgaaaa aataagtggc gatttccact tcccagataa cacaaaaatc
    21661 aattactttt cacaagatct gtactgggaa aatccattag aaacaccact acaatattta
    21721 agcagtcaat ttccaaaagc gactatcaaa gaattacgtc gccaactagc taaagcaggg
    21781 ttagttaatc aattagcaag tgaacctctc actacattaa gtggtgggga acaaactaag
    21841 gtgaaacttg cacagttgac aatgaaccaa ggtaatttac tgattctcga tgaaccgacg
    21901 aaccatatcg atcaagaaac gaaagaaagc ttacaagacg ggatacagaa atttccagga
    21961 acagtaatca tcgtctctca tgaacaagaa ttttatcaag atcttgtgca acgagtcatt
    22021 gagattgaac caaaataaac gtttgattga atagttgaaa aaaaggagct gtgacaaaag
    22081 ttttgtcaca actccttctt ctgaataaat ggtggccaat tagctgacct tcggtattag
    22141 ctcaaaaaac ggcaaaatat gaggagctat ttttatttgg ctattcttat tacttgatgc
    22201 agaacagctt ttcacaacct cttttcgtct attttttatt ttcgagattt ctctttcctt
    22261 taaaaacaac tgtttgaaag aagaacgcac atccttgaaa aacaagcccg ctaaccgtta
    22321 cacctaacca gccatactgc tgccacattg tcgtccctag caatgaacca gccgcacctc
    22381 caataaagta cataaacata aatacagtat tatttcggtt gcttgctgct tcacctagcc
    22441 cttgtactcg tgtttgattg gcgacttgac caaattgatt cccgacgtct aataaaataa
    22501 tagctaaaag caaaatggcc aaatggcttc cgccaaccaa taaagcaata aagcttattg
    22561 tttgcataac aagtcccata tacacgattt ttctttcaga ataagaatct gccagtcgtc
    22621 ccacgatagg tgcagcaact gctccagaca aaccaaaaat cgctaatatc cctgcttcga
    22681 atgttcccca attgtaggcg ggactgctga tataaaaaat caacgtagac caaaagatcg
    22741 aaaatgttcc aaacatgaaa aatccactga ctgccgcttc tcttaataga cgttgagaag
    22801 caactaaatg cggcaaactt ttaagtgaat caaggtatga aacagctgta ctactttgct
    22861 ggacagaacg cggcattttc cagtaaagca gaagtgtaag aattccgact gcacataaag
    22921 caaataaata gatcatccgc caagaaaaaa gactggcaac aataccagat accgtccgag
    22981 aaagcaaaat ccccacgagt aaaccgctaa gcaatgtacc catgaccttc cctcttgctt
    23041 ttggtcccgc tagtaccgca ccgtaaggaa tgatgatttg cggcacgatc gaaagtaatc
    23101 ctattaaaaa agaagcgcat gcaaataacg taaacgaagg cgcaaaaaaa gcggccaaca
    23161 gagagacggc agataacgct gccattcgaa tgatcaattt tggacgatcc accacgtccc
    23221 ctaaaggaac taaaaacagt aaccctaacg catagcctaa ttgggtcaac atcgtcaata
    23281 tcccgactgc acttgtgcta acagataaac tagctgctac ttttgttcca atcggctgga
    23341 tgtaatacat attggcaaca acaacgccac aagtgattga taatagtaaa ataagcgaag
    23401 ttgttaaagg ttttttttca ttactcttca ttgatttccc ccatttccta tcttagttta
    23461 caacttatgg aaaagattgg aaatagaaaa atacaactat tcgccacact tattatttgt
    23521 gcttttgttt tccttgtttt ttcttgcgat ctttataaat gaagacaact aaccctacta
    23581 gcacagtaac acttgcaacc aacgtgactg cagttttgtt atatccgtga aacagagctt
    23641 ctccaccaca cgcatacaac acagacgaag ggatgactcc aatcacgatc gacaatatga
    23701 tttgctgtag aggcaatttc atcgcatctg cagcaaaatt gaccatagaa gatggaataa
    23761 ccggtatcat atagcctaac attacaccta tttttggatg tttcatttgt cggattgctt
    23821 gcacccaatg attcgtttcg gttttcttat ctaaaaattt taagcgatgc ataaggaaaa
    23881 ttgcagtgat gttaccaaga gcattaccca aaatgttgat gatcgcacct gtcagtggtc
    23941 caaagctcaa accagcaatg actccaacta ctgaagtagg aatacctgga acagcacaca
    24001 tcaccgctgt caatacgatc aagagtatcg atgcaaaagt cccatgcgaa cgaatatgtg
    24061 cgagtagctc tgccttactc gctctcggat ttaaaaataa ttggatgtct gtctgatatt
    24121 ctaaatacaa ccgatagccc actaccagta tcagaatgat tcccaacgag atcaatatca
    24181 atcggattat tttctttttc atatttcctc ctgaaaaaaa tctatattca ttaaataata
    24241 gtttaacata aaaacaaaaa agcaccgaaa tccaacatag gatctcagtg cttttaaata
    24301 gtaaaaatta tttaacagtt acagatgcgc caacttcttc aagtttagct ttcaattctt
    24361 cagcttcttc tttagatacg ccttctttaa ctggtgcagg agcgccatca actacagctt
    24421 tagcttcttt caagcctaaa ccagttgctt cacgaactgc tttgataact ttaacttttt
    24481 ggtctccagc tgcagttaat tctacagtga attctgtttg ttcttcagca gcagcagcgc
    24541 caccagcagc agctacagct acaggagcag cagcagatac gccaaattct tcttcaatag
    24601 ctttaactag gtcgttaagt tctaggatag ttgcttcttt taactcagca acgatgtttt
    24661 caatgttcaa tgccattata atttcctccg tttaattgtt cttttttatt taatgcttta
    24721 tgcggcaatt aagctgcatc tccatcttct ttgtctgcca ctgctttgac agcgtaagcc
    24781 acgttgcgga ctggcgcttg tagtacagat agcaacatag atagaagtcc ttcgcgattt
    24841 ggcaattttg ccaatgctgt gatttcttcc aatgaagaga ctttaccttc gatgataccg
    24901 cctttgattt ctaatgcttt tgcttcttta gcaaattcgt ccatgatttt cgctggtgcg
    24961 actacatctt cgttactgaa tgcaacagct gtaggtccag tgaatacttc gtccatgcct
    25021 tcaaggccag cttttttagc tgcacgtgaa agaatagaat ttttgataac tttcatttct
    25081 acgttagcat cacgaagttg tttacgtaaa cgtgttactt catcaactgt taatccgcgg
    25141 tagtcaacga ctactactga tgcagctgct gtaaattttt cgcttacttc atcaacgatt
    25201 gctgcttttt ttgcgataat tgcttcactc atttttgagt ttcacctcct gaattttgat
    25261 ggaagagttt tttgcactga aaaggcaagt cacttttata gttactttgc tcaaccagta
    25321 ttattcatca cctattcact tcgttcatag tgattctact cgctttgctc gaaaggaaac
    25381 tcctttcgct ttgctcaacc agtattgttc atcatctctt cacttcgttc atagtgattc
    25441 acaataaaaa actctatgcc accgcagaca tagagggact attgaatata ttcaataaac
    25501 gtcctcggta ggaaattaag gcttgatgcc acctactgtc ttcggtacaa gttaattatt
    25561 aacctcaact agcttagcat gctaaactag ttgagtcaag aattattttt cagttggatc
    25621 aatcaacatt ctaatattta attagaaaga agcttggtca acatggatac caggtccaaa
    25681 tgttgttgtg atcgttacat ttttcatgta ttgaccttta gctgctgatg gtttagcttt
    25741 tagcaataca tcatggattg ctgcaaagtt ttcaactaat ttttcatcgt caaatgaaac
    25801 ttttccgatt ggtacatgga tatttccagc tttgtccaca cggtaagtta ctttaccagc
    25861 tttcacttcg tttactgctt ttgttacgtc cattgtaact gtaccagttt tagggtttgg
    25921 cattaagcct tttggtccta atactcgtcc tagacgacct acagtagcca tcatatcagg
    25981 tgttgcaaca actacatcaa agtcaaacca tccgttttgg attttttgaa ccatgtcgtc
    26041 gtcacctacg aaatctgcac cagctgcttc agcttctttt gctttgtctc ctttagcaaa
    26101 aactaaaaca gtttgtgttt taccagttcc gtttggtaat acaacagcac cacggatttg
    26161 ttggtctgct tttttagggt ctacgttcaa acggtatgca acttcaactg ttgcatcaaa
    26221 ttttgcaatg tttgtttctt ttgctaaagc tactgcttcg gcaacaggat aaactttact
    26281 tgaatcaact ttttttaatg catcttgcat ttttttgctc tttttagcca ttttggttcc
    26341 tccttgattg tggttataac ggttgtacct cccacgtgtt ctatatcttt caatatagag
    26401 ccaactgaga agctgctgtc ttagggtgta tagattattc tacagtgatc cccatgcttc
    26461 gtgcagttcc ttcaaccatg cgcattgctg cttcaacgtt agctgcgttt aggtcttcca
    26521 tttttaattc agcgatttct cttacttgat cgctagaaac tttcgcaact ttatttttgt
    26581 ttggttcgcc tgaacctttt tcgattttag ctgctttttt caatagaaca gcagctggcg
    26641 gtgtttttgt aacgaatgtg aatgaacggt cttcgtatac agaaataaca accggaatga
    26701 tcaaacctgc ttgatcagct gtacgagcgt tgaattcttt tgtgaatccc atgatattga
    26761 tacccgcttg acctagtgca ggacctactg gtggagctgg agttgcttta cctgcaggaa
    26821 tttgcaattt aacgattttt tctactttct ttgccacgag acatacctcc ttaagtccgt
    26881 gatgtggtta attggagatg catatttctc ctcccacgtt ttatctgatc tgaaaaaatc
    26941 cagacaaata atgtgcaaaa caatgcacac tggtacagta tagcaaattt atttgttttt
    27001 tcaagatttt tttctgctat ttttacattt tgcttgaata aacgctaaat ttattctaaa
    27061 aaaacaacaa tttttcgttc tcatttcttc ttttatgtta cacttgcgct agcatggttc
    27121 aacagatgaa agcaaacaat aacacggttt tttagaccct tgtacaaaga ttcctacata
    27181 acaaaatgag gtggagaaaa tgaaagattt aaggtataga agtgtctttg atattattgg
    27241 acctgtgatg attggtccaa gtagctctca cactgccgga gctgctagaa tcgggaaaat
    27301 catacgtagt atatttggcg aacagcctga ttctgtagat atttatctat atgaatcatt
    27361 tgcaaaaacc tatcgggggc acggaacaga tattgcatta gttggcggcc tgttagggat
    27421 ggaacccgat gatgaacgtt tagctgattc tttgaaattg gcatatgaag ccggtatgga
    27481 agtcttgttt gttcctaaga gtgaaaaagc cgaacatcca aactctgtaa agatgcttct
    27541 aaaaaaagga acacgaaagc tttcagtcac tggcatttcg atcggcggtg gcaatatcca
    27601 gatttctgaa ttgaatggat tcaaaatttc tttaagcatg ggtactccta ccttgatcgt
    27661 tgtccaccaa gatgttccag gtatgattgc taaagtgact agtatacttt ctgagaccaa
    27721 tacgaatatc ggcactatga cagtgacacg ggaatcaaaa ggtgaaaatg ccattatgat
    27781 aattgaagta gatgatcctc aagtagaaga aacggtaaaa agactcaaac agctgccaaa
    27841 tatcgacagc gtaaattatt tcgattaagg agctaaaaaa atgtttttat cgattgaaga
    27901 attagtagat caagcaaaag cttatccaag tgtagcagaa ttgatgattg cagttgaaat
    27961 ggaaatgagt ggccgttccc gtagtcacat catcgagatc atggaaaaaa acctctcagt
    28021 aatggaacaa tcaatcaacg agggaaaaga aggtgttacc tccgtcacag gaattaccgg
    28081 tggtgatgca aaaaaattag atgattattt aaatgaggca gactttttaa gtggagaaac
    28141 aatcttaaaa gctgtacgca atgctgtagc tgttaatgaa gtcaatgcaa aaatggggtt
    28201 gatttgtgct acaccgactg ccggaagcgc gggtgttgtt cctggtgtgc tgatggctgt
    28261 ccgagatcgg cttgatttga cacatgaaca gcaattggaa ttcttattta ccgctggcgc
    28321 tttcgggtta gtcatcgcaa acaacgcttc tatcagtggt gcagaagggg gatgtcaagc
    28381 agaagttggt tctgcaagtg caatggcagc agcagctttg gtttgtgcaa aaaatgggac
    28441 tcctcatcaa gcagcacaag cagtagccat tacattgaaa aatatgatgg gactgatttg
    28501 cgatcccgtt gcaggcttag ttgaagtccc gtgtgtcaaa cgtaatgctt taggctcttc
    28561 tcaagctttt atctctgctg atatggcatt agcgggtatc gaaagtgtca ttccgccgga
    28621 tgaagtcgtt gctgcaatgt accaagtcgg acgtcaaatg ccgtcgattt tcaaagaaac
    28681 agcagaaggt ggcttagctg ttacgccaac tgctaaacga cttgcggaag agattttaaa
    28741 taagagggcg tgataaagct ccttaatttc gagtattaag agattagtta tcttcataac
    28801 aaaaaacgaa gagaggacta attgcccttc tcttcgtttt ttattaatcc atcaatttcg
    28861 tattttttac ttcatcgatc gtttttgttc ctgcaagttg catcgtgatc gacaattctt
    28921 tgttcaggtg ttcaaaaaca gatgttactc cttctgcccc acctagattc aacccataaa
    28981 tcactggtcg gccaatcgca actaagtcag caccacttgc cagagctttg aagacatgct
    29041 ctcctcgacg gacaccagag tcgaaaatca ctggtacacg tttgttgact acttcggcaa
    29101 tttttggcaa tacttcaaag gacgcaggac ctccatctag ttgacgtcca ccatgattgg
    29161 atacccagat accatctgcc cctgcagaga tagcgattac tgcatcttct ggcgattgta
    29221 ttccttttac gataactgga agatgggtga tttctttgat tgttttgatg tcatctggtg
    29281 tcaagccttg ttttgcagct gcataaattt ctgcgattcc ttttccttcg ccattaccac
    29341 ttgcactttg ttcgctatac gcagctaagt taggcattgg taatggaaat tggaattgat
    29401 tgatgacgtc ttcttcacgg taaccaccga gcgttgaatc tgccgttaaa atgatagctt
    29461 tagcccctgc ttctactgct ttgttcaaaa tgaactcatt gaatctgtcg tctttactca
    29521 tatataattg gaaaaactgc ggtgcgcctg gcgctgcatc ggctgcgtct ttgattgtcg
    29581 tatttgcata agtgctgatg gaaaagattg atccagcagc agctactcct ttagctgtat
    29641 cagcttcccc tttttcatgt gccaatcctt gggccgctga tggcgcttgg ataatcggtg
    29701 tatctaattc aattccgaaa acagatgtat gcaaatcggc tgagtcgatt ccgcgtaata
    29761 tccgtggcat gattttcttt gtattaaaag aagtcgtatt ttctttcatc gtccactcat
    29821 cttcagaacc accgcgaata tagccaaaag ctcctgcttc catgcgctct tttacacgac
    29881 cttctagtta ggcaatgttt acaatatcga ttggatgttc ttcattactt gtatgataag
    29941 tcatagtaga tccttctttc atcttcttga ttacatataa atcatactaa acttacaaaa
    30001 agtaagcaaa catttactct cagatacttt tatcgctatt ctttccaaat aaatcgactt
    30061 acaagctgat ctacttcttg gttttcatga agcatactat gagaggcatt ttcacctgtc
    30121 actacttttt cctctatttc atttccgcgt tgcttcaaca acgcatagac cgctaatgca
    30181 ctgtttaacg gtactgttcc atcactcaag tctgtttcat ctagttgacc agcaatcaag
    30241 aaaaatcgag tggttatagg aatattaccg atcatttgct ggtaatcttg atagcggtta
    30301 gaaacgacag caggcccctt tgctaactca tctgtcagag actgcgcact atcatcgaca
    30361 aaatcattaa aaggcgcgcc aattgctgtt aatgtctgaa cttgtggcaa tgacgtatcc
    30421 tgtccatacg ttcctaaata gcgtagtgca ctaactcctc ccatggaatg ccccactatg
    30481 ttgactttat ctatgccgta gttttctttc agatacaaaa gacaagtttt gatccattct
    30541 gcttgattcc actcgttatt tttattatct tcaaacaata cttggaccat cggattgtct
    30601 gcttttccag acaaatctcc tgttgcttgt atttgtccat ctgttcctac tgttaaaacc
    30661 aattctttta ctgcttgctg atttttttcc aaacgccgca acatcccttt aaaggaattt
    30721 tctgtcccgc tgtagccgtg gaaaaaaagt gttggaatag tggttttttc tatcacttta
    30781 ctggattctg ccgttttttc tttatcagaa acttcagtag tatttatctt cgtcgtctgg
    30841 caaccactta acgcaaacaa actgataagg actaaagctg ctgcagttgc acgtttgcat
    30901 aaatttttga ttggttctgt catacacctc ttcctttcca tatagaaaaa agaaagactt
    30961 gaaaggccaa tcatatgatc gacatttcaa gtctctttat taatcgattt tatctacttg
    31021 atcgaaatca agttctgtac tggtttcacg tccaaacata tcgatgttga ctttcaattt
    31081 ttctttttct ccgtcaactt ctgttacttg tccttctaat ccagcaaatg cgccttcgat
    31141 gattttcacg acttcgccaa cttctacttc tagatcattt tgtcgcaggc tcataccgat
    31201 agaacgcaag atatgattga cttcttctgg aagtaatggt gctggtttgc ttccggctcc
    31261 gtgtgaacca acgaatcctg tcacaccagg tgtgttgcgg acaacatacc atgaagcgtc
    31321 tgtcatgatc atttcaacaa gtacgtatcc tgggaatgtt ttatgaacga tttctttttc
    31381 tttcccattt ttgacttcag tttctttttc ttcaggaaca acgacacgga aaataaagtc
    31441 ggccattttc atactttgcg cacgagattc gatatttgct tttactttgt tttcatatcc
    31501 tgaatatgta tgcaagacat accagttttt ttcaaaagat tccattgaag gctcctcatt
    31561 tcattgtaaa ataaaaaaac ctcagtacac ccgaagtttt tctttcgctt cattatagca
    31621 ctaaaagtac gttgtggcta gcgagttatt ttaaaatcca gccaaataag ctttggatgg
    31681 ctgtatccat tacgtaaaac atcaatccga aaataataga agtttcgatg actactaacg
    31741 tatctttacg gagttgtttt ctgcttggcc aagttacttg tttgatttca tcttttacgc
    31801 tttttagaaa tttcatgaga caacctccct tgctactgtt tatttcgttt ctttatgtaa
    31861 tgtatattga ttacaatatt tacaaaactt attgatttcc aaacgttcgc cgctttttcc
    31921 ttcactgact gattttgtgt agttgcgaga cccacaaacg gaacaagcga gtgctgcttt
    31981 tttctttgcc atttacttct gcccccttgc tgagaatcag acttatactt cataacagta
    32041 tcacgtaact ttttacttgt caatttgtga aataaactat ctattgtttt ggataaaatt
    32101 atgatatgat taatttgaaa ttaagctaaa atgagacaat tggaggtata gcgatgctat
    32161 taaaaacact tgtatataag aaaaaagatc tgacaaccgt tacagagact agtacattag
    32221 aagaagcatt aaaaatttta gaaacttccg gataccgttg tgtgccgatc ttagattctt
    32281 caggtaatat tttccgtgga aacatttaca aaatgcatat ctatcgtcat aaagcaaatg
    32341 gcggtgatat gacgcttcct gtcactcatc ttttgaaaaa cgctacaaag ttcatttacc
    32401 tggatacttc tttcttcaaa gtattcttta cgatcaaaga gcttccttat attgctgtcc
    32461 ttgacagtga aaatcatttt tatggtattt tgacacatag tacgttgctt aatatgctct
    32521 ctcaatcttg gagcgtagaa caaggaagct atgtactgac aattgcttcc acaggaaagc
    32581 aaggagattt ggctgctatt gcaaaaatca tttcaaaaca cagcagcatt gccagctgta
    32641 ttactcttga tatcgaaaaa gacgaataca ttcgacgtac attgatcacg ctaccggcta
    32701 acactgaagc gaaagtatgt gaaacgatcg tcaaccactt agagaaaaag aattataaag
    32761 tggtagaaat tgaaaatttg caaaaccaag aagcataatc aaaaagcgaa aaaagaaaac
    32821 agctaatctg ctttcttttt tcgcttttta taattagaca cgcttttcaa aatcaagcgc
    32881 gcgatctaaa taatacatca atggtgctga aacagccatg acgataaact cacttaatgc
    32941 aagcgtgcca taggttgtcc aaaatgcttg gccacctgtt ggaactaaca tataagcaat
    33001 cagtcccatg ctgatggtaa agaagatcgt gttcaatact aaacgcatct ttacattttt
    33061 cactttgttt tgcaataaag ctgtcaggcc taaagaaagc aatgtttggc cgccaccaaa
    33121 aaccacatcc atgataccaa aaccaaagaa ggcattatag acgatgaccc cgcctaaaac
    33181 tccccaaagc aatttacggt taaagacaac taaatgattc aaactttctg aaatacgaaa
    33241 ctggatcggc cctgaggata caggcgcaac taaaatagtt aacgctagat aaagagccat
    33301 gatgatgcca tttacaacga ttacggataa ccgttgtctg cttttcgttt tttgcatttt
    33361 ctctcctctt ttctcctagt ttttttaacg cgggatggtt gatgaaccgc ggttatgtga
    33421 aacaaacaat aattataaca gacttttcat ttatttcaat ccgtcttatc tgcttttaaa
    33481 aaagcaaaag cttaaacaat gtaaacgttt gctaaaaatc gtgggatatg gtataaagta
    33541 agtgtaaggg gtcataagaa ttcccagtaa agcagacgaa gtaggatctg caagaacaga
    33601 attgttgtga aacttaaata aataaaaaag gatgtgggaa tttgagcaac ctcttactag
    33661 agaacggtct agcaagcagc tgaagatcta agtggacatg ccgaaccttg gaacttctcc
    33721 ttacgtaagt aatggatcat gcgcttgcta ggcttttttg tgtttttcgg tcttcggacc
    33781 tttttcattt tcctagactt ctgctataat aaccaagaat taattttgta acagattttt
    33841 ttgaaaaaac aagtttcttt cattcaaaaa atagaaaaag cttgctaatt attggttaac
    33901 cagcaaacag gagtattaaa atatcttgtt ttattgaata gcaggaggaa atcatgaaat
    33961 tccattatca tatattgatc aatccatcgg caggcagcgg aaatggctat aaagtagcag
    34021 aaagaatttt accggtatta aaaaataagc atattgacta tacacttcat tatagtgaat
    34081 acaaaggaca cgatgctgag attgctgaaa ctttggcaaa agaaacattg attccttgga
    34141 tagaagaaga acagaaaaca atcgatactt tccccctttt gatcatcgta ggaggtgacg
    34201 gcaccttaca tcaggtactt gatactttct atcagatgga agtagaattc ccagttgctt
    34261 atattccagc aggttctgga gatgattttg ctcgaggggc aggccttcca aaaaatccca
    34321 aaaaaggact tcagctgatt ttagcagctc aatcaccaga aaaagtccac ataatggctt
    34381 atgaagaaaa aataagtgaa aaaaaaggaa tagctgttaa taatttcggt ataggattag
    34441 atgcagctat cgtccatgcg acaaatcatt cttccacaaa aaagcgattg aacaaataca
    34501 acttaggatc attttcgtat ttattctcta ttctccgtgc tttattcact caaaaaggat
    34561 ttccaattct ggtagatgca ggaggaaaac aattaagttt ttcaaacgca tttttatgta
    34621 cagcaacgaa gcatcctttt tttggcggag gaatcgcgat tgttccaact gctgatgcgt
    34681 ctaaaccagt cattgatttt gtcgtggtcg aacgaatcaa tttctttaaa atcttgtggc
    34741 tcattctttt acttatacag aaaaaacaat tgaaatcgaa atatttccat cactacacaa
    34801 caaacagatt acgtatcgtt tcaacaacac cgcaacatgg tcaagaagat ggagaagaaa
    34861 tggaaaaacg accttttgat atcacaataa ccacaactaa acagttgttt tggtgttaat
    34921 aaaagaggtt ttgtcagaag ctacatctgg aacaccgttt attcggaaaa aaggactgtg
    34981 acacgacttt tgtcacagtc ctttttttac tatgcagtta attctttgcc caattgggtt
    35041 gccatcgata gtttctttct ttacccaaaa taaattggat cattgaaaac accgcagcaa
    35101 aagcaactaa gacacaacca ttgaaccaaa aagattctgc aaatgtttga ttgtaactga
    35161 gtgtttcact gatcaagtta ttattttgaa caaaccaata tgttggtgtg aatgccgcta
    35221 ttttgctaac catttctggc aagtattctg ctggtacaaa caccccaccg ataaagcaac
    35281 tgcctgtgat aaaaatatct ttgatcccac cgctggtttc tgagtttttt accaatgaag
    35341 tgatacaagc cgaaaagcta atgatcggca ataaaaacaa gaaagtattt aaaatatgat
    35401 accataccgt tatatcccac gaggtgtgga tcactaacag aaccattaga agtaagaagc
    35461 cactgaacaa cagcagcgag taactaatca aactaaaggt tactttgcga ttcaagtttc
    35521 gtttggtaat gggcgcactc tggtttcgtt tactgatctc tttgcgatta aatgctagat
    35581 tgatgacgct gaaaccgcta aaaatggata gaaacaaccc ataagacaag aaattaaagg
    35641 ttgtgcccgt caacagcttt ttcatccgct gactatatcc cgcacttagc tcaacttctc
    35701 cagtaaccga gagtgccatt tgtgtctgtt ccatagctgc atcgattgtt ttatcttctt
    35761 cttgaaacag aatcagtgtt tgcaaaaagg tgttcacttg ctgtgtaaca actgttttag
    35821 aaaaggcatc tgggcgactc tgtatcttga tcgtcggcat ctttcctact tcgacttgtt
    35881 cggtaaagtc tgcaggcaga tgtaagataa tgtttacacg attaaaataa agagcatcat
    35941 cgatttcttt ttgtgaggtt ccatccaatg taacgactgt ttccttgtca ttcaaatatt
    36001 tacgaaggcc atcgactaca ggcgactctt cttcactaat caaggcgatt ttcgcatctt
    36061 gcagagaggt attttcaaca tcaatatttt gcgcaaaaaa gaatgtggca acaacagtga
    36121 taattacacc tatcagtaaa ttcgatttat ttgctttaag caatgtccaa atcgttttat
    36181 aaagcgtcat aacgcacccc tctttccatc caagcatttc ctaacgcaaa aaggaaaata
    36241 aatccgatca accatagaag attggtataa aaactagcca aatcagtgta gaaataaagt
    36301 tt
//
LOCUS       DACEEZ010000018        34330 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20313_2017
            SAMN12639966-rid9879223.denovo.018, whole genome shotgun sequence.
ACCESSION   DACEEZ010000018 DACEEZ010000000
VERSION     DACEEZ010000018.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639966
            Sequence Read Archive: SRR10023158
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 34330)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34330)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741683.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741683.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:44:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,016
            CDSs (total)                      :: 2,962
            Genes (coding)                    :: 2,889
            CDSs (with protein)               :: 2,889
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 36 of 73
            Pseudo Genes (incomplete)         :: 29 of 73
            Pseudo Genes (internal stop)      :: 18 of 73
            Pseudo Genes (multiple problems)  :: 8 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34330
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639966-rid9879223.denovo.018"
                     /isolate="E20313_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            202..1077
                     /locus_tag="IWO89_000986"
     CDS             202..1077
                     /locus_tag="IWO89_000986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0455758.1"
                     /translation="MNNWLEYFPENVLERGYSYHLHGFVRHLNYTSKYLSATVSGTED
                     YKVVITWDEKTNMTCDCLYAIEGKKCKHMAAVLFAYEERPIKKSNYSLSELSSLVSSA
                     SSSLVRELLTEILIEHPQFIERFKVKMPFHAINYSDKLTTIIHKYDHIIKKNKNRKTA
                     KFIMEMRKFIQEAVESLIQQNAYLPAFELINEVIATLETFYWEPEDERTLLLIEDCYY
                     LWKELLAEAPHAEKRQMFSWFVCQVDHTDASYSKRYSIKILKEDFREKEFSNQKKKID
                     KKTKETVKKDDFNEK"
     gene            complement(1222..1374)
                     /locus_tag="IWO89_000987"
                     /pseudo
     CDS             complement(1222..1374)
                     /locus_tag="IWO89_000987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874852.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="xylulose kinase"
     gene            complement(1642..2973)
                     /locus_tag="IWO89_000988"
     CDS             complement(1642..2973)
                     /locus_tag="IWO89_000988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAQ0455759.1"
                     /translation="MGKSSRKDIVFEKFGIVATKLGNQIHLRTLRDAFATFMPFMMLA
                     GFVTLINSVILEPTGFMGEIVDTSTLTTLQQIGTSIANGTLSITTLLVVAAVSYHMCA
                     SRNYTNHIAAVLVAISTFVVLTPMQMMFTPENADKAVEVTGVIPISYTGASGMFVGIF
                     VGLAATELFIKLSNNKKLQIQLSGNIPPAVLKSFNVLIPIIITITSFALLSFIIIQLF
                     NMDINKLITTIVTEPLSKITTGLPGFLLITSIANLFFGFGIHQAVISGSLLDPFLIQN
                     MQENMAAYANKEQIPHIINMAFKDTFAVMGGSGNTIALLIAIFIFSRRKDYKDFAKLS
                     VTPAIFNISEPIIFGLPIVFNISLIIPFVLAPIFSLTVAYFATAVGLINHVVVQIPWT
                     TPPVISGFLATAGDWRAAVLQILIIAASVFIYLPFLRIDEHVTTKLSENET"
     gene            complement(3122..3835)
                     /locus_tag="IWO89_000989"
     CDS             complement(3122..3835)
                     /locus_tag="IWO89_000989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016611336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAQ0455760.1"
                     /translation="MKKIAKYMEIYLDIKKKIVNGEYKIGEKLPTGDELAVIYHTSKL
                     TVKKGVDLLVSEGVLRSRSGYGTEVVRTPIDNSRVLGPNEGLFSVVGEEHVDSEIHTF
                     SIELPSKKVADKLSISVKDYVYNIVRSRFIDCQPYSIEQTFMPLAVIPGLEPKHLKKS
                     VYAYITNELGLEIKTSHIRIKGDTANEFDAKILKIDPGEFMIEIDKVVALSSGKPFEY
                     SVSRHLYQDFVFEAVFVEK"
     gene            4010..5434
                     /locus_tag="IWO89_000990"
     CDS             4010..5434
                     /locus_tag="IWO89_000990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015508962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HAQ0455761.1"
                     /translation="MTTMKKLTFPDDFWWGSAWSAEQAEGKGETGKAETIWDRWFKEQ
                     PYRFYQRIDSSITTDHIHQYKEDIELMKKTGHNSFRVSISWARMFPNGGTGKVNPKAI
                     SFYRKLFSEMNNNGIKVFANLYHFDMPATLQDKGGWESREVVDAYVHYAETCFKEFGD
                     LVCHWFTFNEPLGPILGTYLEDFHYPNLIDFKRGAQAAFFTILAHAKAIQVFKELALP
                     SKIGVILNLSPTFPRSQNPADVKAAEIADLFYTRSFLDPMVKGNFSKKLILLLQEYNQ
                     LPEDYTKEDLVCIYENTAQILGLNYYEPRRVKSRSSMVDTSSPFLPEWFFEPHIMPGR
                     RMNEYRGWEIYEKGIYDLCMDIKNHYGNIEAFISENGMGVADEERFMDDTGQIIDDYR
                     IEYIKDHLAYLWKAINEGCNIKGYHLWTFIDCWSWINAYKNRYGLVSLDLTTQKRTIK
                     KSGEFYKQLSDTNGFRYDVDLLVE"
     gene            5733..6440
                     /locus_tag="IWO89_000991"
     CDS             5733..6440
                     /locus_tag="IWO89_000991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlC family ABC transporter permease"
                     /protein_id="HAQ0455762.1"
                     /translation="MDEKLDIKTAIKDTLPTVFGYIGIGLAFGIVGKAAGFHPLVVTL
                     MSLLIYAGSAQFITVSMLASHSPLLSIVLSTFLVNSRMILMSMTIAPYFKKNRLLQNL
                     LIGTLLTDESFALGMNKLNYTGQKLNFRWMNTANWISYLTWVGSSLVGALLGNFITDP
                     KKFGLDFAIVAMFIGLLYLQVISDRNTSKRLQLILIGLTLILVYVGLIFIPSNLVILV
                     VTLIGCGLGVWIKHAFF"
     gene            6427..6750
                     /locus_tag="IWO89_000992"
     CDS             6427..6750
                     /locus_tag="IWO89_000992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlD domain-containing protein"
                     /protein_id="HAQ0455763.1"
                     /translation="MPSFEFTLLTILGCTLATWLSRILPFVLLKKFDLPQPLLEYLSF
                     VPIVIMSALWFSSLFTQNIGHLPQINLENALASVPTLLAAILSKSLLVIVLVGILSLS
                     LIRLL"
     gene            complement(6839..7876)
                     /locus_tag="IWO89_000993"
     CDS             complement(6839..7876)
                     /locus_tag="IWO89_000993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding alcohol dehydrogenase family
                     protein"
                     /protein_id="HAQ0455764.1"
                     /translation="MSEKELMKVVGFYEGLSIDDPKSFIDEKEQIPVPSSRDLLVKVN
                     AVSVNPVDTKLRQDNGIRNALRILGFDGVGKVVAVGDEVQKFSVGDRVFYAGTTTRAG
                     SNQEYQLVDERIVALAPKNLSDEEAAALPLTSLTAYELLFEKFGLTPEENANRGKKIL
                     VINGAGGVGSILNQLAHWAGLEVYATASPKNFEWLKKTGVDYPIDYHHDLSKSFRELG
                     IDQVEYVAVLFDITRYFDQIKHLVRPFGHVGTIVGIKEPLDIGVWKNQSISFDWEYMF
                     AKTDYNYEIETQGEALAHIAALANEGIIRSTLSKVYSDGMNAKNLKQATKDVETGHIL
                     GKVVVSGPFNG"
     gene            complement(8197..9180)
                     /locus_tag="IWO89_000994"
     CDS             complement(8197..9180)
                     /locus_tag="IWO89_000994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-dihydrouridine synthase"
                     /protein_id="HAQ0455765.1"
                     /translation="MTTNFWKELPKPFFVLAPMEDVTDVVFRHVVKHAAAPDVFFTEF
                     TNSDSFCHPDGKDSVRGRLTFTEDEQPMVAHIWGDKPEFFREMSIAMAEMGFQGIDIN
                     MGCPVPNVAERGKGSGLILRPEVAAQLIEAAKAGGLPVSVKTRIGYTEMSEMEEWISH
                     LLHQDIANLSVHLRTRKEMSKVDAHWEVIPKIIALRDQIAPQTLITINGDIPDRQTGE
                     ELAEKYGVDGIMIGRGIFKNPYAFEKEPREHTEKELIGLLRLQLDLQDHYSEIIPRSI
                     TGLHRFFKIYIKGFPGANDLRVQLMNTKSTDEVREILDTFEKEHPTLFSEQ"
     gene            complement(9595..10365)
                     /locus_tag="IWO89_000995"
     CDS             complement(9595..10365)
                     /locus_tag="IWO89_000995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAQ0455766.1"
                     /translation="MFNLYFTALKSLAIKETNRYLRIWVQTLVPPVITTSLYFVIFGK
                     MIGGRIGDMGGFSYMEFIVPGLIMMSAITSSYANVSSSFFSQKFQKNIEEILVAPVPT
                     HVIIWGFVIGGVGRSILVGSLVTIISLFFVPLHVYSWFMVIITLLMTAILFSLAGLLN
                     GIFAQSYDDVSIVPTFVLQPLTYLGGVFYAISMLPPFWQAVSKVNPIVYMISGFRYGF
                     LGTIDVPVATSMIVLVLFIVVLYAVTWYLINKGRGLRS"
     gene            complement(10358..11293)
                     /locus_tag="IWO89_000996"
     CDS             complement(10358..11293)
                     /locus_tag="IWO89_000996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ0455767.1"
                     /translation="MANALEINNLKKVYGSGVEALRGIDLSVKEGDFYALLGPNGAGK
                     STTIGIITSLVNKTSGKVNVFGYDLDTDLVRAKQQIGLVPQEFNFNPFETVQQIVVNQ
                     AGYYGVSRKEAMKRSEKYLKQSNLWEKRNVRARMLSGGMKRRLMIARALMHEPRLLIL
                     DEPTAGVDIELRREMWEFLRELNENGTTIILTTHYLEEAEMLCRNIGIIQSGELIENT
                     SMKELLSKLQFETFIFDLAPYETKPVIEGYACTFEDEQTIAVEVERNQGVNGIFDQLT
                     RQGIKVLSMRNKSNRLEELFLKITEENNHTEEKNV"
     gene            complement(11609..13258)
                     /locus_tag="IWO89_000997"
     CDS             complement(11609..13258)
                     /locus_tag="IWO89_000997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HAQ0455768.1"
                     /translation="MGQREFIVLIIIAAVILLVALDIFSRLKLKETVRSKWEKIPYQP
                     RFDKEESLKEAWLTEKKFRSWDSEIDDLTWYDLDMFEVFEGINSTYSSVGSEALYQRL
                     RSFDFGEDQQLEKLIAFYQENPQLREKIQYQFARLGKKDHNFAKQYLADGKSKKIGRV
                     GLFIFLGLLPLVGLGLLLLGQVLGIYLAIGGLVFNTVYYLVKKQTLETELNSMSYLVS
                     TISAANQLAKLPTPLQEELKQQTKPLRDIPKLGFSFRMKSGSEAELIFEYVNIMFMLP
                     FISYHFVLTKLEKHSTEAIKLWELLGELEVAAAILNYRTYMPITCRPEFTEGGVTAKD
                     IYHPLLKEAVVNPVNWQKNTLVTGSNASGKSTYVKSIAISCILAQTIQTAIAEQFTLQ
                     PGHVLTSMAIEDDLFEGDSYFVSEIKSIKRLLDQVASKERCYCFVDEILKGTNTIERI
                     ASSASVVRWLAEYPSLAFVATHDIELTEILKNYCENVHFEEQVTEGKGISFDFKLKEG
                     PSRTRNAIALLKVLDYPETLVETAKTEAALFDEKRQWHVLG"
     gene            complement(13375..15978)
                     /locus_tag="IWO89_000998"
     CDS             complement(13375..15978)
                     /locus_tag="IWO89_000998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-mannosidase"
                     /protein_id="HAQ0455769.1"
                     /translation="MVNAHIIQHTHWDREWYFTNEDAIVLSDQVFTEVLDELERNPKV
                     NFCLDGQTSIVDEYLEINPQKLKVIQRLMKTGQLFVGPWYAQTDALLVDAESILRNLM
                     IGISDTILKYGDPMMVGYLPDTFGFNANLPTLLHQVEIDNFMCWRGLNFETMVPSPYF
                     IWKGLGDKFVYAMNFPFGYMTGMMTLDAIKDIPEFAKEKLDEAVEMLHMQGKNKDILV
                     PSGVDQKSMILNFDQVVEKLNKYSKFHNIISDYPSYVEVIRNKKELPEYKGELRQPVY
                     SRVHRSIGSVRTKMKIENFKLEQKILKRVEPLMLIAERNGIHISKGLLRRLWKKILQN
                     QAHDSIGGCVSDNVAEDIFHRIKEANEIADGIENLIVKRMADALKLKSNEVLVFNTTT
                     ENYSGSKIVHVVADSKKIHFKGSPYNIIEKEVYFPERKNAYRMIATGFEYFDEPAYYE
                     LDIKINVNVPALGYTVVEFEKNNEKLETAIEIDKTEISNNKYKLSFKDGQLNLIVGDR
                     QYLDFVHLIDSANDGDTYDYSPLEGDTELSLKLETAKVYKDSLQETLVVYGKAQLPKD
                     LKDRLSEKPEMEEISYEISFSLGESQIVEGTLKIHNTVYSHRMRLAINVLDKSEKSIA
                     QIQNGFIENTPDVIPPNWSETMVEKPVNLEIFDKSVSVAENDHYFTFYADGLKEYERI
                     ENQLIITLFSTTGELGKPNLAWRPGRASGDTTNEGHVMMETPLAQEIGEYKVTFGFNV
                     EEGRFNEFKVAKQAEKRLEQSISYQKQKLNVFIHRLDNKIWPLQNELDVPRKFSLIEL
                     PEDLIVSSIYPSYFHEQGFVLRLANPTEQEKIVPEAILSLGTVVNALENKQELTTIPP
                     YDYLSILINER"
     gene            complement(15990..17039)
                     /locus_tag="IWO89_000999"
     CDS             complement(15990..17039)
                     /locus_tag="IWO89_000999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIC"
                     /protein_id="HAQ0455770.1"
                     /translation="MEFKKQGREILNHFQSGVSYMIPLVVAAGLLTSIAVIFGGTGVW
                     DQTDTFWGVLRMIGQTGLQFIVPMISAYIAYSIADRPGLAPAFITGMMCQNLGMGFIG
                     GMVAGLACGYLANALKKVKVPMQVISLKSIMLIPFVTTLVVGIILWYVLATPIQAMTT
                     GLTNWLEGMSGANAALMSAILGGMMAFDMGGPINKIAYAFGVASFSSGSFGPSTAMLL
                     GIAIPPFGMFLATILNKKLYDESERDNAKTAMIMGLVGITEGTIPFAVKDPLRVIPSI
                     VVGTAVACGLNGFFGVTQETMLSTFMAIPFVTNIPLYLVSIVIGGVVTALMVNALKSI
                     KYKKEQQVNSVEEGK"
     gene            complement(17065..17379)
                     /locus_tag="IWO89_001000"
     CDS             complement(17065..17379)
                     /locus_tag="IWO89_001000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIB"
                     /protein_id="HAQ0455771.1"
                     /translation="MTKIVAVTACIAGIAHTYMAKANLEKFAKKKGLDIKVETQGAMG
                     IENKLSISDVSEADVVIFAVDTNVSERGRFDGKPILTVGTSEVVKDGEETIDEAIALI
                     NK"
     gene            complement(17395..17841)
                     /locus_tag="IWO89_001001"
     CDS             complement(17395..17841)
                     /locus_tag="IWO89_001001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIA"
                     /protein_id="HAQ0455772.1"
                     /translation="MISEKININNIVIDLNASDKKSVVEKLSKLLETNGFVSDHKQFQ
                     KDVFDRENHMTTGIGKNIAIPHGKSLAVIESTVAVAKLKRSVEWGALDDQPVDLVFLL
                     AIKDTDKGTNHLRILAELSGRLMDDEFVKRIKDSRNKKELVKALSF"
     gene            complement(17842..19818)
                     /locus_tag="IWO89_001002"
     CDS             complement(17842..19818)
                     /locus_tag="IWO89_001002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HAQ0455773.1"
                     /translation="MKIEIIIVHLSCIQEVKIMGIEFSERQMKILYFLIENEHTTYKE
                     LSEQLYVSTKTISKDILILKEIIQKMPDVQMLVQPKKGVSLIGKKETLRNLLSSHKIN
                     IADSPEDRLTYVFAKLLNTNDSLKIQNLADELYVSRSTVESTMKDVRKRLNKHGMSLV
                     SDRNGVRIKADEKSKRRLMSEVVNYYWGGFTASNSKTDLLDLNINFIGEANNFLNKDI
                     LKKVAELLNRYMNKTQLKFTDYEFQTLAIHLAIAIERIETSNFLSRSNNSEEEISKNT
                     LILIKMIEKAFSIIVPIDEQAYVDIHITSIEKNAMNHDLDQTKLSYLDMGVELQELVR
                     IKLNEFYPDEELINNLVLHLNAAVKRLKLGVNIYNPYTDKIKYSFKRSFMISVDLLEE
                     IEERFCIHFNEDEIAYVTLHIQSLLDRYKPDKTKVILVCSSGYGTSKLLEQRINNGFA
                     DMVEIEDVLSINELQDRKVTDELVISTIPIENTNFPVIVVSPLMLGADVRKVKQYLQL
                     NEQTEDAFLNLLDKRFCFFTHQIKDNQVNVLNIITKKLVKENYAKEGILEGAIKREKL
                     SSTAMNDFAMPHIEVDFIKKPVICVYVNPEGIDWDGTLVHSVFFFALNEKVKPELNKI
                     YETFNEIASNHELLANLKTSSSFEKLVYILKEGL"
     gene            complement(19976..20872)
                     /locus_tag="IWO89_001003"
     CDS             complement(19976..20872)
                     /locus_tag="IWO89_001003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HAQ0455774.1"
                     /translation="MIKWLLLLVVIFMGSFIFFLQREIKRLTKKIATLSERQEYGGRL
                     SVEFREKNLMDLVDALNTWLDENEQKGQAFQEMEENIRLSIVGLSHDLRTPLTAINGY
                     VQLLEQVEDTEKREKYLTIIKQSVARLLSMTDQFYDLARIETKQKEMDLHTLSFSKEV
                     EEVFFSFYEQFSEKNIEVAFAEHSINTTVLADPLMLLRVLQNIIQNLLRYADGQAKVT
                     FSKEDPFIRLTVANPIKPGTQTSVEKVFQRFYTENTSRTNTESSGIGLYLSKKLVEGM
                     GGEMTARLDGGIFSISVKLRRV"
     gene            complement(20865..21545)
                     /locus_tag="IWO89_001004"
     CDS             complement(20865..21545)
                     /locus_tag="IWO89_001004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAQ0455775.1"
                     /translation="MVLLETILIIEDDEAVHSLLEEVLEQRYKLLDAYSGTEGKLLLS
                     TYPVDLILLDLMLPGLSGEALLAEIRQTSNVPIIVLSAKSDQRDKVSLLAAGADDYVT
                     KPFDIEELLLRIGIQLRHQTSVQVKDLSQRIYYKEILLDKESRDVNVNGVAVHLTGRE
                     FDLLKLFLEHPKKVFSRANLYETVWQEPYFDSEKTVNMHISNLRGKLAKAHAVYIHTV
                     WGVGFRFD"
     gene            complement(21546..22328)
                     /locus_tag="IWO89_001005"
     CDS             complement(21546..22328)
                     /locus_tag="IWO89_001005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HAQ0455776.1"
                     /translation="MFNLLTAEKIKVWKSRKMWVSLGLMVVLPLFYSWNEWYMHTKYG
                     NELNQATDTVINGATGILMVEKMAAWILLAFAAFACFYIGEEFQNGTIRNTLSLGRNR
                     MTYYVSKLLVTLLITTLGVVLITGLAMLAYTLAFGFGEVEGIKNYGNYVLKVFPVLLL
                     LTLGTLSVPVALTFITRSTSVSLLLSFLYIMGTAFIPGVFAKIKGLEFLTEWFTETWL
                     MYTDFAQQATYSQAPKMILVSLVTIVVSSALGMFIFQKSDIK"
     gene            complement(22321..23238)
                     /locus_tag="IWO89_001006"
     CDS             complement(22321..23238)
                     /locus_tag="IWO89_001006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAQ0455777.1"
                     /translation="MDFGNETVLKATGITKKYGAAKALDKVSIEIKRGMIYGLIGENG
                     AGKSTFMRTIMGLISIDEGSIELFGTTDLQAARRRMGQSIETPALYPELTARDNLRIQ
                     AANGGVSDREIEDLLKMMRLENTGKKKAKNFSLGMRQRLAIANALITNPEFLILDEPT
                     NGMDPAGMAEMREIIQRLVKERGITVLLSSHLLDELSQIATHYGILHEGHLIKELSKE
                     ELAQESRQFIKIDTSATEQAVTVLDSLGYRDYFVQSSRVIQLFEGIDQVAAINQALVE
                     AKVPVDGIHLVGQKLEDYFLQLTGGNPHV"
     gene            complement(23402..23725)
                     /locus_tag="IWO89_001007"
     CDS             complement(23402..23725)
                     /locus_tag="IWO89_001007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAQ0455778.1"
                     /translation="MFSFAFYMVWLQREVPTHIQGRLMSTITTITMVASPLGLALFGA
                     LFDWKTTQPRLRDSLIFLGGSLAIVILITGLVKLLKLDLKEAKIFSEEEYRETTLVEK
                     KESGN"
     gene            24513..27740
                     /gene="fss3"
                     /locus_tag="IWO89_001008"
     CDS             24513..27740
                     /gene="fss3"
                     /locus_tag="IWO89_001008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010729285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fibrinogen-binding MSCRAMM adhesin Fss3"
                     /protein_id="HAQ0455779.1"
                     /translation="MYKKQKKKLCVLTILSLAVQAIVGAIAPTITFADEITHPQTVTV
                     ELDLAHQYAVEGTFSDGRPMSEVTVPHYAVYNGFKQDIFCIEPGVPIYNEFTPGYEKN
                     PLPDMSDKAKLVSVLWKKVGTDADTHIVAQKMIWQEVNGYTLHSIKRSDGSAVNIAAI
                     EAKINQAIDDYQKKPTFHNSTAKTVLGQSTTVTDTNNLNLSEFDEVVENAANIDYRVN
                     GNQLIITPNANSKESGVLTLKKSAGTGTPVAYKKAGQQTLMAGAIDKPNTYTIKIDVE
                     TEGSLKIKKVDKESGDVVPGTVFHLDFGKTLPAKDVTTDKEGIATLDEIPHGTKVTIT
                     EKSVPAPYTIDTTPMTTTIKAGETIYVTSKNAREKGQIILDKSGVETGSDLWNDNYSL
                     AGNTFAIRKDSPTGEIVQEMTTDENGHAETPKEIANALELGTYYVTETKASHGFVNTF
                     KPVKVELKYANQTVAPVTSNVKGQNQEVTGETTLTKEDKDTGDKAQGKAVFEGAEYTL
                     FTAKDGKAVKWSEAFKPELAKGTKGSDETVTLTLDEKNQVAVKHLAINEYYWQETKAP
                     EGYSLDETKYPVSIKKVDDSEKNAVITRNVTAKEQVIRFGFDFFKFAGSAAGTTETGF
                     NDLTFKVSPLEGTNEITGAEDEATTAYNEQLGFDGYGKFENLPYGDYLLEEVEAPEGF
                     QKITPLEIRSTFKENKEDFVKSEYVFTITEQDQKQPIKTVTVPYEKLTNKAFSVSLNR
                     LMLYDLPEEEDSLTSLATWKDGNKELTSLDSTELVDKLSYNLHEIKEDWYVVAQAIDV
                     DATKAAQEKDEKAKPVVIAETSATLANKEKTGTWKIQHKLTAEQVLNKTIVLFNYVYE
                     NKEAFEAGDKPVAKDVSLNNQAQTVSCTVEHHVSIQTKAHLENGSQTFTHGDVVDMFD
                     DVSITHDVLDGSKEAFETILYALLPDGTNKEIWKSGKIDYEVNDKEFTKTVLAKKVDT
                     GKYPEGTKFTFAEINYDKDGNINGKHNEDLKEKSQTLTPKEVPTILSTPKQPETPTVP
                     SGSQESSPTVKTFPQTGEKNSNVLLFIGFTLIFATAGYYFWNRRN"
     gene            27853..28167
                     /locus_tag="IWO89_001009"
     CDS             27853..28167
                     /locus_tag="IWO89_001009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HAQ0455780.1"
                     /translation="MELKFVVPDMAETFGKLSYAGEGEIITEGYGRNTTVIGRSYHLY
                     SSKQRADDIEVVVAAEAGEKDFDQDQPLKAVNPHLVAKGYEIENRGFTDYVLYVDDLV
                     KA"
     gene            28180..28554
                     /locus_tag="IWO89_001010"
     CDS             28180..28554
                     /locus_tag="IWO89_001010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HAQ0455781.1"
                     /translation="MRLTEGIVVDSGLTFGKLRFSALRREVRKQNEDGTIGNEVKERT
                     YNLKSSAQGRMIQVSIPANVPLREFAYDAEVELVNPIVDTVANYVFREGTTVNWFIKA
                     DDLVLKRQPNQGNPTNQNERKK"
     gene            28564..28899
                     /locus_tag="IWO89_001011"
     CDS             28564..28899
                     /locus_tag="IWO89_001011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="damage-inducible protein J"
                     /protein_id="HAQ0455782.1"
                     /translation="MEINVEHILDCLDQYGKGELTEEQLVKALTSDEKMFLIIHQGLL
                     GENSDSEEDSDVLDWLGEEESFFIVVEVNENLCRQAESILEEIGVEMPDAIEGFLNQL
                     VETKQLPSS"
     gene            28982..30322
                     /locus_tag="IWO89_001012"
     CDS             28982..30322
                     /locus_tag="IWO89_001012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010759325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF87 domain-containing protein"
                     /protein_id="HAQ0455783.1"
                     /translation="MYKGHRIRAGDQHLVYHFVLGWLLALFIGWMSVFYFQEFRQFDI
                     SKLSLSTIEIVWSIKDLVCLLGSLAFSGAMILLYIHFFLDHWRSLWHRQKLARMILEN
                     HWYEVKQTQSEGFFKDLNSSRTRETISYFPKICYRMKDGLLSIRVQISLGKYQDQLLK
                     LEKKLESGLYCELVEKELKDSYVEYTLLYDMIANRIGIDEVVAENGTLRLMKNQVWAY
                     DSLPHMLIAGGTGGGKTYFLLTIIEALLKSDAELFILDPKNADLADLGTVMPHVYSQK
                     EEISACVEDFYERMMARSKAMKEMSNYKTGENYAYLGLPPNFLIFDEYVAYMEMLTTK
                     ESAVILNKLKQIVMLGRQSGFFLILACQRPDAKYLGDGIRDQFNFRVALGRMSELGYS
                     MMFGEVEKNFFMKHIKGRGYVDTGGSVISEFYTPLVPKGYDFLDSISKLEKMND"
     gene            30400..31074
                     /locus_tag="IWO89_001013"
     CDS             30400..31074
                     /locus_tag="IWO89_001013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HAQ0455784.1"
                     /translation="MESNDSYLEYAKANKDKFISYIIKGKSPEVGRDAVFMAGSPGAG
                     KSEVAIGLAENYDNHVIIDADYFRTQFPDYNGKNSSLFQKASSWLVEQAFRYVLDHGY
                     SFILDATFAILSAEKNISRALKKHFRVTIFYVYQDPKIAWKFTQEREIAEGRHVPKKA
                     FINAFFKSRENIAKVKSKHPDVLLHIIIKDYTNNISEVHYATDNLELFLPLGYSRKDL
                     EVQLHD"
     gene            31307..31741
                     /gene="dcm"
                     /locus_tag="IWO89_001014"
     CDS             31307..31741
                     /gene="dcm"
                     /locus_tag="IWO89_001014"
                     /EC_number="2.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA (cytosine-5-)-methyltransferase"
                     /protein_id="HAQ0455785.1"
                     /translation="MRFLDLFAGIGGFRLGMEQASHHCIGFCEIDKFARRSYKAIHDT
                     SKEVEMHDITSVSDEFIQSLGPVDILCGGFPCQAFSIAGKRQRFSDTRGTLFFEIARF
                     TTLLRPKLLFLENVRGLLNHEGGATFETILRTLDGLGYDVEW"
     gene            31742..32449
                     /locus_tag="IWO89_001015"
     CDS             31742..32449
                     /locus_tag="IWO89_001015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA (cytosine-5-)-methyltransferase"
                     /protein_id="HAQ0455786.1"
                     /translation="MLNSKAYIPQSRERIFLIGHSRDACTEQVFPIIGPSSTSDQNIR
                     NLVNINPSNRGMGGQVYGSDGVAPTLTGDEGIKIALPARDGISVAGLLPGKFEQGNRV
                     YEVTGTAPTLSTKQGGTKIMIRKNETEYQEVKPEDSVNLAFPNSTSRRGRLGKQSVHT
                     LLTGDQQAIVTDQYQIRKLTPRECWRLQGFPDWAFDRDSQVNSDSQLYKQAGNSVTVP
                     VIFDIARRLKELDVHGL"
     gene            32439..32729
                     /locus_tag="IWO89_001016"
     CDS             32439..32729
                     /locus_tag="IWO89_001016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002395166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0455787.1"
                     /translation="MVYDELIGEIYWVIGKIQSDPELEEQLRRLKFDIRKNGVKVAGD
                     PYLMNEETDARIEINQVIAEFERIADQAKEPDIRQYLLEMKAELEVNGITDE"
     gene            32988..34172
                     /locus_tag="IWO89_001017"
     CDS             32988..34172
                     /locus_tag="IWO89_001017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HAQ0455788.1"
                     /translation="MAQRNLDYRLLKDRRNEYGVSQNKLATACGLSRPYLNQIENGGV
                     TASIKTMRKIFDQLESFNPDLPLTLLFDYVRIRFPTTDARKIIQEILHLKFEYMLHED
                     YAFYSYQEQYVMGDIVVMLSHEEDKGVLLELKGRGCRQFETFLLAQKRSWYDFFEDCL
                     KAGGVMKRLDLAINDRVGLLDIPELTKKCQKEECISLFRTFKSYRSGELLKADEKDGM
                     GNTLYIGSLKSEVYFCLYEKDYEQYIKLGIPLDQTETKNRFEIRLKNDRAFHAIQDLL
                     KGRSIESTTFSIINRYLRFADKVEGKRRTNWPLNEQWGRFIGRNRKEIQLTSEPKPYT
                     IERTLNWLGRQVAPTWKMAKELDRLNRTTYIQDMVQNARLSDRHKKILEQQSMAIENL
                     II"
     gene            34187..34306
                     /locus_tag="IWO89_001018"
     CDS             34187..34306
                     /locus_tag="IWO89_001018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3789 domain-containing protein"
                     /protein_id="HAQ0455789.1"
                     /translation="MGIVKDIFLVFGGSMLGVTVMCLMNASAAADRAMEKKGK"
BASE COUNT        10701 a         6609 c         6254 g        10766 t
ORIGIN      
        1 ataaataaaa aacaaaaaaa gttccatctt taaaacacgc tattctttta ctttttctca
       61 cattctctaa gaaaagaaaa ggagaatgaa tttttgacaa ttcgagcaaa agtgattatc
      121 aaatatgtaa aatcagctac ttttttctta aacgtttctt tatactaaat ataaataagt
      181 gtttcatgaa aggagaacta gatgaataat tggcttgagt attttcctga aaacgttttg
      241 gaacgcggtt acagctatca tctgcacggt tttgtccgtc atttgaacta tacaagcaag
      301 tacttgtccg caacagtttc tggcacggaa gattataagg tagtgatcac ttgggatgaa
      361 aagaccaaca tgacttgtga ttgtctatat gccatagaag gaaaaaaatg caaacacatg
      421 gctgctgtct tattcgctta cgaagaacgt ccaataaaaa aatccaacta ctcactttcc
      481 gaactctcgt cccttgtttc ctccgctagt tcctctcttg tacgggagct tttaacagag
      541 attcttatag aacatccgca atttatcgaa cgcttcaaag ttaagatgcc atttcatgca
      601 ataaattatt cagataaatt aacaactatc atccataaat acgatcatat tatcaagaaa
      661 aacaaaaaca ggaaaacagc gaagttcatc atggagatgc gaaagttcat ccaagaagca
      721 gttgaatcgc tcattcagca aaatgcctat cttcccgcat tcgaattgat caatgaagtg
      781 attgctactc tcgaaacatt ctattgggag cctgaggatg aacgaaccct tcttctgata
      841 gaggactgtt attatttatg gaaagaatta ttagcagaag cacctcatgc agaaaaaagg
      901 caaatgttct cttggttcgt ctgccaagtc gatcatactg acgcctctta ttcaaaacgc
      961 tactccataa aaatcctgaa agaagacttt cgggaaaaag aattttctaa ccaaaagaaa
     1021 aaaatagata aaaaaactaa agaaacggta aagaaagacg atttcaatga aaaatagcaa
     1081 gtaaacgata acaaccatcc taaatccaca agaaatacag cagaccttgc tttaaaaaca
     1141 cgaatcatgc aaaggatgaa gtaaagaaga cttaacgcgt acccaatatt agtcatttga
     1201 ctattaggta cgcgttttgt tttataagtc atgacaaatt tccttcgttg ctgggtagat
     1261 tttccggcat tgttgataga ttttatgata cttttctaca ttttgtaaga gaggttccac
     1321 tgcatctgta tattggataa aattctgaca acaactttca ggtgtgtcga accactctaa
     1381 tctttaaaga taaatattaa aataacgatt aataatatta acagtcttta tttttcacca
     1441 ccaaaaacct atctttgcat atactaaata gacgtatata cacagatagg tttgtcttgt
     1501 gaaaaataat gattatcctt accaaaaaat gtttgttttt ctacatgttc gtacttatca
     1561 aaaaatatga tccttattga aattactggc tatttatatt ccaacgactt cttgtgttaa
     1621 tttttgttat ctcatagtaa attatgtttc attttcactt aatttagttg tcacgtgttc
     1681 gtcaattcgt aaaaacggta aatatataaa cacacttgcg gctataatta atatttgtaa
     1741 aacagctgct cgccagtctc cggctgttgc taaaaatccc gatataactg gtggcgtagt
     1801 ccaaggaatt tgcactacta catgattaat taatcccaca gcagttgcaa aatatgctac
     1861 tgtcaaagaa aaaatcggtg caagcacaaa gggaataatc agactgatat taaatacaat
     1921 aggcaatcca aaaataatag gttcgcttat attgaatata gcgggagtta ccgataattt
     1981 agcaaagtcc ttataatctt ttctacgact gaagataaat attgcaatca ataaagcaat
     2041 cgtatttccc gatccaccca ttaccgcaaa ggtatcttta aaggccatat tgataatatg
     2101 tggaatttgc tctttgtttg cataagctgc catattttct tgcatattct gaatcaaaaa
     2161 cggatctaac aatgaacctg atattactgc ttgatggata ccaaatccaa aaaatagatt
     2221 agcaatagat gtaattaaca aaaatcctgg taaaccagtt gtaattttac ttaatggctc
     2281 tgtaactatc gtggtaatta gcttatttat atccatatta aacaattgaa tgattataaa
     2341 tgacaataaa gcaaacgagg taatcgtaat aatgattggg attaatacat taaaagactt
     2401 taaaacagcg ggaggaatat ttccggatag ctgaatctgt agtttcttat tattagaaag
     2461 tttaataaat aattctgtag ccgctaatcc aacaaatata cctacaaaca ttcctgaagc
     2521 tcctgtatag gatataggta tcacacccgt cacttcaaca gctttatccg cattttcagg
     2581 agtaaacatc atctgcatag gtgttaatac aacaaatgtc gaaatagcaa ctaataccgc
     2641 tgcaatatga tttgtataat ttctgcttgc acacatatga taagatactg ctgctacaac
     2701 taataatgta gttatgctta atgtaccatt ggcaattgaa gtaccaattt gttgtaacgt
     2761 ggtcaatgta ctagtatcga caatttcccc catgaatcct gttggttcta agatcactga
     2821 attaataagt gtcacaaatc ctgctaacat cataaatggc ataaatgtag caaacgcatc
     2881 tcgcaaagtt cttaaatgga tctgattccc caacttagtc gcaactatac caaatttttc
     2941 aaatacaatg tccttcctgc tacttttgcc catatttttt ctcctttatg cttattgtaa
     3001 tcgattactt tttccaccca aataatacat cacttatcaa aaaaagtaaa tacttttaat
     3061 taaatatata tatctttttt agtataaaaa aataagctct gaaaaaataa ttttcccgag
     3121 cttatttttc aacaaaaaca gcttcaaaaa caaagtcttg atataaatgt ctcgataccg
     3181 aatactcgaa aggtttacca gacgatagag caacaacttt gtctatctca atcataaatt
     3241 cacctggatc tatttttaat atttttgcgt caaattcatt cgcagtatct cctttgatac
     3301 gtatgtgcga agtctttatt tccagaccca gttcatttgt gatataagca tatacagatt
     3361 tctttaaatg ttttggttcc aagcctggta tgactgctaa tggcatgaat gtttgctcaa
     3421 tcgagtatgg ttgacaatca ataaaacggc ttcgtacaat attatataca tagtctttta
     3481 cactgatact taatttatca gctacttttt tcgatggaag ttcaattgag aaagtatgga
     3541 tttctgaatc tacgtgttct tcaccaacca cgctaaacaa accttcgttt ggaccaagaa
     3601 ctcgtgagtt atcaattggt gttctgacga cttcagtccc atacccactt ctacttcgca
     3661 aaactccttc ggaaacaagt aagtctacgc ctttctttac tgttaacttg cttgtatgat
     3721 aaataactgc caattcatct cctgtaggaa gtttctcacc aattttatac tctccattta
     3781 caattttttt ctttatatct aagtaaattt ccatatactt cgcaattttt ttcaatggtg
     3841 accctccaat caaaaacata tcttcatcta tactttaaca taaataaacc gataaaatcc
     3901 acatcaaaat tatacacaca ataaaaaaga tatatatatt taaacaaaac tatttacttt
     3961 aagttgtaag cggtgttatc attaagtgga aacaggaggt aaaaaaatta tgacaactat
     4021 gaaaaagcta acttttccag atgatttttg gtggggttcc gcttggtcag ctgagcaagc
     4081 agaaggaaaa ggagaaacag gaaaagcaga gactatctgg gatcgctggt ttaaagaaca
     4141 accttaccgg ttttatcaac gaatcgattc ctctattaca accgatcaca tccatcaata
     4201 taaagaagat atagaactaa tgaaaaaaac tgggcataac tcctttcgcg tttctatttc
     4261 ttgggcacga atgtttccaa atggcggtac cggaaaagta aatcccaaag ccatttcttt
     4321 ctatcgaaaa ttattttccg aaatgaataa taacggaatc aaagtatttg ccaacttata
     4381 tcattttgac atgcctgcta ctcttcaaga taaaggagga tgggaatcac gtgaagtagt
     4441 tgatgcttat gttcattacg ctgaaacttg ttttaaagaa tttggtgact tagtttgtca
     4501 ttggtttacc ttcaatgaac cacttgggcc tattttaggt acttatcttg aagattttca
     4561 ttatccaaac ctcattgact ttaaacgcgg agcgcaagca gcattcttca ctatacttgc
     4621 acacgcaaaa gcaatacaag tatttaaaga actagctctt cctagtaaaa taggcgttat
     4681 tctaaatttg agtcctactt ttcctcgtag tcaaaatcct gctgatgtga aagcagcaga
     4741 aatcgctgat ttattttata cgcggagctt tttagatcct atggttaaag ggaatttttc
     4801 taaaaagctt atcctgttat tacaagaata taatcagctt cctgaagatt atacgaaaga
     4861 agatcttgta tgtatctacg aaaataccgc tcaaattctt ggactaaact attacgagcc
     4921 acgtcgcgtc aaatctcgtt cttctatggt ggatacatct agtccatttt taccagagtg
     4981 gtttttcgaa ccacatatca tgcctggacg acgaatgaac gaataccggg gatgggaaat
     5041 ttacgaaaaa ggaatttatg atttgtgcat ggatatcaag aaccattatg gaaacatcga
     5101 agcatttatc tcggagaacg gaatgggcgt tgcggatgaa gaacgtttca tggatgatac
     5161 agggcaaata attgatgatt atcggattga gtatatcaaa gatcatcttg cttacttatg
     5221 gaaggcaata aatgaaggat gtaacattaa ggggtatcat ctttggacat ttattgattg
     5281 ctggtcatgg atcaacgcct ataaaaaccg atatgggctg gtttcactcg accttactac
     5341 tcaaaaaaga acaattaaaa aaagtggcga attttacaag caattaagtg atacaaatgg
     5401 atttagatac gatgtagact tgttagttga ataatattct aaaattccac ttgaagtgaa
     5461 cacattagaa aacaagaata atgagaaata ccaataacaa atactttttc ttcatacttc
     5521 atttatactt gtcagtataa gagactgtga caaatgtagt gatcctcaga agttaggtct
     5581 ggaaatctaa cttgagaaga tttttttaaa ctacgtccag tcttttttat ttgtttaaaa
     5641 ttgaagttga caagtcttca ttaaaaattt agaatattca caagtttgac gacaattatg
     5701 aaaatttatg tcgtgctaaa ggagttttgt tattggatga aaagttagat ataaaaactg
     5761 cgatcaaaga cacgttacct accgtttttg gttatatcgg tattggactt gcatttggta
     5821 tcgttgggaa agctgccggg tttcatccgc tggtcgtcac gttgatgtcc ctgctgatct
     5881 atgctggttc tgcccaattt atcacggtca gcatgcttgc tagccatagt ccactgctct
     5941 ccatcgttct ctcgaccttt ctagtcaatt cccgaatgat tctgatgagt atgacgattg
     6001 ctccttattt caagaaaaat cggctgcttc aaaatctgct gattggcaca ttgctgacag
     6061 atgaaagttt cgcactggga atgaataaat tgaactatac tggacaaaag ttaaactttc
     6121 gttggatgaa tacagctaat tggatttctt atttgacgtg ggtgggttct tctcttgttg
     6181 gcgcactttt agggaatttt attactgatc cgaaaaagtt tggtttggat ttcgccatcg
     6241 tcgctatgtt tatcgggtta ctttatcttc aagtcatttc tgatagaaac acctctaaac
     6301 gattacagtt gatcctgatt ggtctcactt tgattctggt atatgtcgga ctgattttta
     6361 tcccaagtaa cttagtgatt ttagttgtaa cactgattgg gtgcggattg ggggtgtgga
     6421 tcaaacatgc cttcttttga gtttacattg ctgactattt taggttgtac tttagcaact
     6481 tggctttcta ggatcctacc atttgtctta ctgaaaaaat tcgatttgcc gcaaccgttg
     6541 cttgagtatt taagttttgt cccgatcgtc attatgtctg ctttatggtt cagcagtcta
     6601 tttacccaaa atatcggaca tctgccgcag atcaatctcg aaaatgcttt ggcttcagtt
     6661 cctacacttt tagctgctat tctttcaaaa agcttattgg tgatcgtctt ggtaggaatt
     6721 ctttcccttt cgttgattcg cttattataa ataaaaaaac aagagcttat ggcaaattac
     6781 tgtcatgcgc tcttgttttt atcaaagtca aataagaaaa aaatcggcgt attcttgctt
     6841 atccgttgaa cggaccacta acaacgactt ttcctaatat atgtcctgtt tctacgtctt
     6901 tggttgcttg tttcaagttc ttcgcattca tcccatctga atagaccttg ctcaatgtac
     6961 tgcggataat cccttcattt gctaaagcag caatgtgcgc taaagcttct ccttgcgttt
     7021 cgatttcata gttatagtct gttttagcga acatatactc ccagtcgaag ctgatagatt
     7081 ggtttttcca aacacctata tctaacggct ctttgatccc tacgatcgtt cccacatgtc
     7141 caaatggtcg gacaaggtgt ttgatttgat caaagtagcg ggtaatatcg aataatacag
     7201 cgacatactc tacttgatcg attcctaatt cacgaaaact tttacttaag tcgtgatgat
     7261 aatcgattgg atagtctaca cctgtttttt tcagccattc aaaattcttc ggacttgcag
     7321 tcgcgtagac ttctaggcca gcccaatgcg ctagttgatt caaaatcgaa ccgacgcccc
     7381 cagctccgtt gatcactaag atttttttcc cgcggttggc attttcttct ggagtcagac
     7441 caaatttttc aaataacaat tcataagcgg tcaatgaggt caacggcaag gctgcggctt
     7501 cttcatctga aagatttttt ggtgcaagag cgacaatacg ttcgtcaacc agttggtatt
     7561 cttgattgct tcctgctctt gtcgttgtac ctgcgtaaaa aacgcgatcg ccgacagaaa
     7621 atttttgcac ttcatcacct acagcaacaa ctttcccgac tccatcaaac cctaagatac
     7681 gtaaagcatt tcttatcccg ttatcttggc gaagtttcgt atcgacagga ttaacagata
     7741 ctgcgttgac ttttaccaga agatcccgtg aactgggtac aggtatctgt tctttttcat
     7801 caataaagct ttttggatca tcgatcgaca acccttcata aaaaccgact accttcatta
     7861 attccttttc tgacatacga attcctcctt agcttcatca agttggagca tccatacagt
     7921 tttcatttca atatatatag tatgctattt gttccacttt tattcatcat aattattctc
     7981 ttacttaaag taaactattt gcactttttt ggttaaaaaa gaggttgcga caagactttt
     8041 gtcacaacct cttttaaacg gtgttcaatc aactgaccct cggtattcgc tcaaaaaacg
     8101 acaaaatatg aggagctatt tttgtttggc tgttcttatt acttgatgca ggacagcttt
     8161 ttcacgacct ctaattcacg gtgttcaagc aagaaatcat tgttcactga aaagcgttgg
     8221 atgttctttt tcaaaggtgt ctaagatctc tcgtacttca tcagtagatt tcgtattcat
     8281 caactgaact cgcaagtcat ttgctcctgg aaatcctttg atatagattt taaagaaacg
     8341 atgtagtcct gtgatggaac gcgggatgat ttctgaataa tgatcttgca gatctagttg
     8401 cagacgaagt agtccgatca gttctttttc tgtatgttcc ctcggttctt tttcaaatgc
     8461 ataaggattc ttgaaaatcc cccggccaat catgatgcca tctacgccat atttttcggc
     8521 caattcttct cctgtctggc gatctgggat atctccgttg atcgtgatca gtgtttgcgg
     8581 agcgatttgg tcacgtaaag caatgatttt agggatgact tcccaatgtg catctacttt
     8641 actcatctct ttacgcgtac gcaaatggac agaaagattg gctatatctt gatgaagtag
     8701 gtgactgatc cattcttcca tctcagacat ttccgtatag ccgatacgcg tcttgacgct
     8761 gacaggcaaa ccgccagctt tagccgcttc aatcaattgt gccgctactt ctgggcgaag
     8821 gatcaagcca ctgccttttc cacgttctgc tacattaggg actgggcagc ccatgttgat
     8881 atcgattcct tggaagccca tttctgccat agcaatgctc atttctctaa aaaattctgg
     8941 cttgtctccc caaatatggg caaccatcgg ctgttcatct tctgtaaagg tcaatcttcc
     9001 tcgtacactg tcttttccat ctggatgaca gaaactgtct gagtttgtaa actcagtaaa
     9061 gaagacatcc ggtgctgctg catgctttac gacatggcga aatacaacat ctgttacatc
     9121 ttccattggt gctaaaacaa aaaaaggctt tggcagctct ttccaaaaat tagtagtcat
     9181 ttatttgtta ctctccattc tatcaagcag tgaagtcgca atctttctca aaaacattgt
     9241 cattatacta ggaaacaaaa aaagaagcaa agtgaacagc ttaaaagatg tttctgattg
     9301 agcaaaaaaa gactgtgaca aaaatcttat cacaattcct tttccggaat aaatggtgtt
     9361 caaaagcttg catctgcggt aataagtcca accaaacgaa aaataggagg agctattttt
     9421 gtttggctgt tcttattact tgatgcagaa cagctttttc acaacctctc cactaaacgg
     9481 tgttcaatca gctgaccctc ggtattagct caacaaacga caaaatatga ggaactattt
     9541 tcccgtttgt ttctctaata ctcaggtcaa aacgctgatt tcacaacctc ttttctaact
     9601 tctcaatcct cttcctttat tgatcaaata ccaagtaacg gcgtacaaaa caacgatgaa
     9661 tagcacaagt acgatcatcg atgttgctac tggcacatcg attgttccaa ggaatccgta
     9721 acggaaaccg gagatcatat aaacgatcgg attgactttc gaaacagctt gccaaaatgg
     9781 cggcaacata gagatggcat agaaaacgcc acctagataa gttaacggct gcaagacaaa
     9841 tgtaggcacg atcgatacat cgtcataaga ctgagcaaaa atcccgttga gcagtcctgc
     9901 taacgaaaac aagattgccg tcatcaataa tgtaatgatg accatgaacc atgaatacac
     9961 atgaagtggt acaaagaaca aggaaatgat cgttacgaga gagccgacga gaatactgcg
    10021 acccactccg ccaatcacaa agccccaaat gatcacatgc gtaggaacag gcgctaccaa
    10081 gatttcttcg atgtttttct ggaatttttg agagaagaag gacgaagaaa cgttggcata
    10141 cgaactagtg atagcagaca tcataatcaa accaggcacg atgaattcca tatatgaaaa
    10201 accacccatg tcaccgatcc gaccaccgat catttttccg aaaataacaa agtacaagga
    10261 tgtcgtgatc acaggcggaa ccagtgtttg gacccagata cgcaaatagc ggttcgtttc
    10321 cttgatagct aaacttttta atgcagtaaa ataaagatta aacatttttc tcctccgtat
    10381 gattattttc ttctgtgatt ttcaaaaaca gttcttctaa acgattcgac ttattcctca
    10441 tagaaagaac tttgattcct tgtcttgtca gctgatcgaa gattccgttc accccttgat
    10501 ttcgttcaac ttcgacagcg attgtctgtt catcttcaaa tgtacaagcg tatccttcaa
    10561 tcacaggttt ggtctcatat ggcgctaaat caaagataaa tgtttcaaat tgcagtttgg
    10621 atagaagctc tttcatacta gtattctcga tcagttcacc tgattggatg atcccgatgt
    10681 tgcgacaaag catttcagct tcttctaaat aatgcgtcgt caaaatgatc gttgtaccat
    10741 tttcgttcaa ttctcggaga aactcccaca tctcgcgtct caattcaata tcgactcctg
    10801 ctgttggttc atccaagatc agcaaacgtg gttcatgcat caatgcccta gcaatcatca
    10861 gtcttcgctt cattcccccg gaaagcatac gtgcacggac atttctcttt tcccaaagat
    10921 tggactgctt caaatatttt tcgctgcgtt tcatcgcttc tttgcgagag acaccgtagt
    10981 agcctgcttg attcacgacg atttgctgga ccgtttcaaa gggattgaaa ttgaactctt
    11041 gcgggactaa tccaatctgt tgctttgctc ggactagatc cgtatccaga tcataaccga
    11101 agacattcac ttttccagat gttttattga ctaaagaagt aatgattccg atcgttgtag
    11161 acttcccagc tccatttggt cctaatagcg catagaaatc tccttctttc acggaaagat
    11221 cgattcctcg caatgcttcg accccacttc cataaacctt tttcaaatta tttatttcaa
    11281 gcgcatttgc catttatttc ctatctcctt atagacatga ttctttattg tatcatggaa
    11341 tacagcagat gaaaaaacaa cttggttcca gcgaaaaatc aggttgctgg gctcaggaaa
    11401 atctcctaat gaaagacatc aatcggacga agtcagacaa ccttttcaga aaaaacaaat
    11461 cgaagataaa ggattactat tcatatgtgc aacaaaagga gcgtgagaca aaagtaaaga
    11521 acacttttgt ctcacgctct aaacacgtat aaacggcgga agcaaaagcc aagatttttg
    11581 atccaataaa tactttcgat cacttcgcct atcccaatac atgccactgc cgcttttcat
    11641 caaataacgc ggcttccgtt tttgcggttt ctactaaagt ctctgggtaa tccaaaactt
    11701 tcagcaaggc aatcgcattt cgggtccttg aaggtccttc ttttaatttg aaatcaaaac
    11761 tgattccttt tccttctgtt acttgttctt caaagtggac attctcacaa taattcttta
    11821 aaatctctgt caattcgata tcatgtgtgg cgacaaatgc taaagatgga tactccgcaa
    11881 gccaccgaac gactgaagcg gaagaagcaa tccgttcgat cgtatttgtc ccttttaaaa
    11941 tctcatctac gaagcagtag cagcgttctt tggaagccac ttggtccaac aaacgtttga
    12001 tcgatttgat ttctgaaaca aaataactgt cgccttcaaa taaatcatct tcgattgcca
    12061 tggaagtcaa cacatgtcca ggttgcaggg taaactgttc tgcaatcgct gtttggatcg
    12121 tctgtgctag aatacagctg atggctatac ttttgacata cgtagatttt cctgatgcat
    12181 tggaacccgt gactaatgta ttcttctgcc agttgaccgg attcactacc gcttctttta
    12241 acaatggatg atagatatct ttcgctgtca caccgccctc agtaaactct ggtcggcaag
    12301 tgatgggcat ataagtacgg taattcaata ttgcagcggc tacttctaat tctcccagca
    12361 attcccacag cttgatggct tcagttgaat gcttttctaa tttagtgaga acgaaatgat
    12421 aggagataaa tggcagcatg aacatgatgt tgacatattc aaaaatcaac tctgcttcac
    12481 tcccactctt catacgaaat gaaaaaccta atttagggat gtctcttaat ggtttagttt
    12541 gctgtttcag ctcttcttga agtggtgtcg gcagttttgc tagctgattg gcggctgaaa
    12601 tcgttgaaac caaataactc atgctattca gctctgtttc tagtgtttgt ttcttcacta
    12661 gataatagac cgtattgaat actaacccac cgattgccaa atagattcct agtacttgtc
    12721 ctaaaagaag caaacccaaa cccaccagcg gaagcaagcc aagaaaaata aataaaccta
    12781 cgcgtccaat ctttttactt tttccatccg ctagatattg ttttgcaaaa ttatgatctt
    12841 tttttcctaa acgagcgaat tgatattgga ttttttctct cagttgagga ttttcctgat
    12901 aaaaagcaat cagtttttct aactgctgat cttcaccaaa atcaaagctg cgtaaacgtt
    12961 gatacaacgc ttctgaacct acactagaat aggtactgtt gattccttcg aatacttcga
    13021 acatatccag gtcataccaa gtaagatcat cgatttcgct atcccatgaa cggaacttct
    13081 tctccgtgag ccatgcttcc ttcaagcttt cttctttatc aaatctcggt tgataaggaa
    13141 ttttttccca tttgctacga actgtttcct ttaatttcaa acgtgaaaaa atgtctaaag
    13201 caactaataa aataacagct gcaataataa ttaaaacgat aaattctctt tgacccatat
    13261 ccctctccgt tcctttattt gttagcttta gtatagcact gttgcgctat ttgaaaagag
    13321 ggtaaaaaaa cacctttcaa aataaaaggt gtttttttct tgtatattac atatttatct
    13381 ctcatttatc aaaattgaca aataatcata gggaggaatt gttgttaatt cttgtttatt
    13441 ctccagtgca ttgacaactg tcccaaggga aaggatagcc tcaggtacta ttttttcttg
    13501 ttctgttgga ttagctaagc gaagaacaaa tccttgttca tgaaaataag aaggataaat
    13561 cgatgataca attaagtctt caggtaattc aatcaatgaa aatttacgag gaacatcaag
    13621 ttcattttgt aatggccaaa tcttgttatc taacctgtga ataaatacat tcagtttctg
    13681 cttttgataa gaaatagatt gttctaaccg tttttctgcc tgttttgcca ctttgaactc
    13741 attaaatctt ccttcttcta cattaaatcc aaaagtgact ttatactcac ctatctcttg
    13801 agctagcggt gtttccatca tgacatgccc ttcatttgtc gtatctcctg aagctcgccc
    13861 aggtcgccaa gcaagattag gttttccaag ttcccctgtt gttgaaaata aggttatgat
    13921 taattgattt tctatacgtt catactcttt taaaccatct gcataaaacg taaaataatg
    13981 atcattttct gccacagaga cactcttatc gaatatttct aaattaacag gtttctctac
    14041 catagtttct gaccaattcg gaggaatgac atcaggggta ttttcaataa atccgttttg
    14101 aatttgtgca attgattttt cagatttatc taaaacatta attgctaagc gcatacgatg
    14161 cgagtaaaca gtattatgaa ttttgagtgt gccttcaacg atttgacttt ctcccagact
    14221 gaacgatatt tcataagata tttcttccat ttcgggtttt tcagataaac gatcttttaa
    14281 atctttagga agttgagctt ttccgtaaac aactaatgtt tcttgtaaag aatctttata
    14341 tactttagct gtttctaatt ttagagatag ttctgtatct ccttctaatg gtgaataatc
    14401 atacgtatcg ccatcatttg ctgaatcgat caaatgaacg aaatcaagat attgtctatc
    14461 tccaactatc aaattcaatt gcccatcttt aaaagaaagt ttatatttat tgttacttat
    14521 ctcagttttg tctatctcga ttgctgtttc taatttttca ttatttttct caaattcaac
    14581 aacagtgtat cctagtgctg gcacatttac atttattttt atgtcaagtt cataataagc
    14641 tggttcatca aaatattcga aacctgtagc aatcatccga taagcatttt tccgttctgg
    14701 aaaataaact tctttttcaa tgatattata gggactacct ttgaagtgta ttttctttga
    14761 atcagctact acatgcacga ttttcgatcc actataattc tcagtcgttg tgttgaaaac
    14821 aaggacttca ttcgatttta gctttaatgc atcagccatc cgttttacaa taaggttttc
    14881 gattccgtca gctatctcat tagcttcttt gattcgatgg aaaatatctt ccgcaacatt
    14941 atctgaaaca cagccaccaa tactatcatg agcctggttt tgcaaaatct ttttccataa
    15001 ccgacgaagt aggcctttac tgatatggat accatttctt tctgcaatca gcatgagcgg
    15061 ctcaactcgt tttagaattt tttgttccaa tttaaaattt tctattttca tttttgttct
    15121 tactgagcca atagaacgat gaacacgaga gtaaaccggc tgacgaagtt cacctttata
    15181 ttcaggtaat tcttttttgt ttctgataac ttctacataa gaaggataat ctgaaataat
    15241 attgtggaat ttactgtatt tattcaactt ctctacgact tgatcaaaat ttaaaatcat
    15301 tgacttttga tctacacccg aaggtactaa aatatctttg ttttttcctt gcatatgaag
    15361 catttcaact gcttcatcca acttttcttt tgcaaattca ggaatatctt taattgcgtc
    15421 taaggtcatc attccagtca tataaccaaa tggaaaattc atggcataaa caaatttatc
    15481 tcccaatcct ttccagataa aataaggaga tggaaccatc gtttcaaagt ttaatcctcg
    15541 ccaacacatg aaattatcga tttctacttg atgcaaaagt gtaggcaaat tcgcattgaa
    15601 cccaaacgtg tcaggcaaat accctaccat cattggatct ccatatttaa ggatcgtatc
    15661 actgattcca atcattaagt ttcttaaaat tgattcagca tccactaata aggcatctgt
    15721 ttgtgcatac cacggtccca cgaatagttg tcctgttttc atcagtcgtt ggataacctt
    15781 aagcttttgc ggattgatct ccaaatactc atcgacgatt gaagtttgtc catctaaaca
    15841 aaagtttact ttagggttcc tttccaattc atctagaact tcagtaaaga cctgatcaga
    15901 caatacgatg gcatcttcat ttgtaaaata ccattctctg tcccagtgcg tgtgttgaat
    15961 tatatgtgca tttaccatat cagattctcc tactttcctt cttctactga atttacttgt
    16021 tgttcttttt tatacttgat ggattttagt gcattgacca ttaacgctgt aaccactccc
    16081 ccaataacaa ttgatactaa atataaaggt atattcgtaa caaaaggaat tgccataaag
    16141 gttgaaagca ttgtttcttg agtaacacca aaaaatccat ttagaccaca tgctactgca
    16201 gtgcccacca cgatgcttgg tattactcgt aaaggatctt tcacggcaaa cgggatcgtc
    16261 ccttcagtaa tccccacaag gcccataatc atagctgtct ttgcattatc tcgttccgat
    16321 tcatcataaa gttttttgtt taaaattgtt gctaaaaaca ttccaaatgg cggaatagca
    16381 attcctaata acatggctgt actaggtcca aagcttccgc ttgaaaaaga agcaacccca
    16441 aaagcatatg cgattttatt gataggtcct cccatatcaa acgccatcat tcctcctaaa
    16501 atagccgaca tcaaagcagc atttgctcct gacatgcctt ctagccaatt agtcagtcct
    16561 gttgtcattg cttgaatagg cgtagctagt acataccata atatgattcc aacaactaat
    16621 gtagtaacaa aaggaatcag cataattgat tttagactaa taacttgcat cggtactttt
    16681 acttttttca atgcattagc taaatagcca caagctaatc cagcaaccat tcctccaata
    16741 aatcccatac caagattttg acacatcatc ccagtgataa aagctggtgc tagaccggga
    16801 cggtcagcaa ttgaatatgc gatataggct gaaatcatcg gtacgataaa ctgtaatccc
    16861 gtctgaccaa tcattcgcag gactccccag aatgtatccg tctggtccca aacacctgtg
    16921 ccaccaaaaa tgactgcaat agaagttagt aaccctgctg ctacgacaag cgggatcata
    16981 taggaaaccc cactttggaa atgatttaga atttctcttc cttgtttctt gaattccatt
    17041 agtaactccc ccacgatata ttttttattt gttaattaat gcaattgctt catcaatcgt
    17101 ctcttcacca tcttttacta cctctgaggt ccctactgtt aaaatcggtt taccgtcaaa
    17161 gcgacctcgt tctgaaacat ttgtatctac tgcaaatatg acaacatctg cctcggaaac
    17221 atccgaaatt gaaagtttgt tttctatccc catagccccc tgtgtttcta ctttaatatc
    17281 taaccctttc ttcttcgcaa acttttctaa attagctttt gccatataag tatgggcaat
    17341 ccctgcgata catgctgtta ctgctactat ttttgtcata tgaaattcct ccttttaaaa
    17401 tgatagagct tttaccaatt cttttttatt tctcgaatct tttattcttt taacaaactc
    17461 gtcatccatc aatcttccag ataattctgc taaaatacgt aaatgattag tacctttatc
    17521 agtgtctttt attgctaaca aaaagacgag atcaactggt tgatcatcta atgcgcccca
    17581 ttcaacagac cgctttaact tggctacagc cactgttgat tcaattactg ctaggctttt
    17641 gccatgagga atggcgatat ttttcccaat tcctgtggtc atatggtttt ctctgtcaaa
    17701 tacgtctttt tgaaattgtt tgtggtcaga cacaaaccca ttagtctcta acaatttcga
    17761 taatttttcg actacacttt ttttatcaga tgcatttaaa tctatcacta tattatttat
    17821 attgattttt tcactgatca tttataagcc ttctttcaat atataaacta atttttcaaa
    17881 cgacgaactc gtttttaaat ttgctaataa ctcatgattc gaagcgattt cattgaatgt
    17941 ttcataaatt ttattcaatt caggttttac tttttcatta agtgcaaaaa agaaaacaga
    18001 atgaacaagt gtcccatccc aatcaattcc ttctggatta acgtagacac agattactgg
    18061 cttttttata aaatctactt cgatatgcgg catagcaaaa tcattcatcg ctgttgaaga
    18121 tagtttttct cttttgattg ctccctctag tatcccttct ttcgcgtaat tttcttttac
    18181 caattttttg gtaattatat ttaaaacgtt cacttgatta tcctttattt ggtgggtaaa
    18241 aaagcaaaat cgtttatcta ataaatttag aaaagcatct tctgtttgtt catttaactg
    18301 tagatactgt tttacttttc tgacatctgc ccctaacatc aaaggactaa caacaataac
    18361 aggaaaattt gtattttcaa tcggaatcgt gcttataaca agttcatcag tgacttttct
    18421 atcttgtagt tcattgatgc ttagtacatc ttctatctca accatatctg caaaaccgtt
    18481 atttattctt tgctctaata attttgatgt tccatatccg ctactacaaa ctaatatcac
    18541 tttcgttttg tcaggtttgt aacgatccaa aagtgattga atatggagtg ttacgtaagc
    18601 gatttcatct tcattaaaat gtatacagaa tctctcttcg atttcctcta ataaatctac
    18661 acttatcata aatgaccttt taaaagaata tttgattttg tccgtataag ggttatagat
    18721 atttactccc aatttgagtc ttttaactgc tgcattgaga tgtaatacta gattattgat
    18781 caattcttca tcaggataaa attcatttaa tttaattctt actaactctt gtagctcaac
    18841 acccatatct aaataggaaa gcttcgtttg atctaagtcg tgattcatag catttttttc
    18901 aatcgaagta atgtgaatgt caacataagc ttgttcatct attggcacaa taattgaaaa
    18961 tgctttttca atcattttga ttaaaataag tgtattcttt gagatttctt cttcagaatt
    19021 attactacgt gataagaaat tgcttgtttc aatcctttct atcgcaatag caaggtggat
    19081 agctaaagtt tgaaattcat aatctgtaaa tttcaattgt gtcttattca tgtatcgatt
    19141 aagcaattcg gctacttttt tcaatatgtc cttatttaag aaattattcg cttcaccaat
    19201 gaaattgata ttcaagtcta agagatctgt tttactgtta gatgctgtaa agccacccca
    19261 ataatagttc acaacttctg acatcaacct tcttttactt ttttcatcag ctttgatgcg
    19321 cacgccattt cgatctgaaa ctaaggacat cccatgcttg tttagtcttt ttctaacatc
    19381 tttcattgtg ctttctaccg tcgacctgct cacgtataat tcgtcagcaa gattttgaat
    19441 ctttagagaa tcattagtat tcaataattt agcaaacaca taagttagac ggtcctccgg
    19501 agagtctgcg atattgattt tatgactgga taataaattt cgaagtgttt ctttttttcc
    19561 aataagcgaa acgccttttt taggttgaac caacatttgt acatctggca ttttttggat
    19621 tatttctttc aaaattaaaa tatctttgct aattgttttc gtagatacat ataattgttc
    19681 tgataattct ttgtaagttg tgtgctcatt ttcaatcaaa aagtatagta ttttcatttg
    19741 ccgttcgcta aattcgatgc ccattatctt cacctcctga atacagctta agtgtacaat
    19801 tattatctct attttcatac agaagaaagt tcttgattaa tgtagaagtt tctaatattc
    19861 ttgattattt caaatcaata aaaaagcctg caatatttat atcacaggct tttttctcta
    19921 ttaaatatat ataagataat acatttttct actagttctc actaatcttg tataattaaa
    19981 ccctccgcaa cttgacggag atggagaaga tgcccccgtc taatctagcg gtcatctctc
    20041 ctcccattcc ttccaccaat ttttttgaca ggtaaagccc gattccgcta gattccgtat
    20101 ttgtgcggct ggtattttcg gtataaaaac gctggaagac tttttcaaca gaggtttgcg
    20161 ttcctggttt gataggattg gccactgtta atcgaatgaa tggatcctct tttgaaaatg
    20221 tcacctttgc ttgtccatct gcataacgca ataaattctg tatgatattt tgcagcactc
    20281 gcagcaacat gagcggatct gctaatacag ttgtatttat cgagtgttca gcgaacgcta
    20341 cctcgatatt tttctcagaa aattgttcat aaaaagagaa aaatacttct tccacctctt
    20401 tagaaaaaga aagcgtatgg agatccattt ctttttgctt cgtctctatt cgagcaagat
    20461 cataaaactg atccgtcata ctgagtaatc ttgcaacaga ttgtttgatg atcgttaaat
    20521 acttctcccg tttctccgta tcctctacct gttccagtaa ttgtacataa ccgttaatag
    20581 cagtcaaagg agttctgaga tcatgagaaa ggccgacgat cgatagtctg atgttttctt
    20641 ccatttcttg aaatgcttgt cctttttgtt cattttcatc caaccatgta ttcagtgcat
    20701 ctactaggtc cattagattc ttttctcgaa attcgactga aagacgacca ccatattctt
    20761 gtcgttcaga aagagtggcg attttcttag ttaatctctt gatttccctt tgcaggaaga
    20821 aaataaaact tcccataaag ataacaacga gaagcaacag ccatttaatc aaatctaaac
    20881 cccactcccc acaccgtatg aatatacaca gcatgtgcct ttgctagctt ccctcgaaga
    20941 ttggaaatat gcatattcac tgttttctca ctatcaaaat atggttcttg ccaaactgtt
    21001 tcataaagat ttgctcgcga aaatactttt ttcggatgtt ctaaaaaaag cttcagcaga
    21061 tcgaattctc ttcctgtcaa atggacagcc acaccattca catttacatc cctcgactct
    21121 ttgtccagta agatttcctt gtaatagatg cgttgcgaca gatcttttac ttggacagaa
    21181 gtctgatgac gaagttggat gccgattcgt aacaaaagtt cttcgatatc aaatggtttc
    21241 gtcacatagt catccgctcc tgctgccaac aaggaaactt tatctctttg atcgcttttt
    21301 gctgaaagaa cgatgatcgg aacattcgat gtctgtcgga tttctgctaa caatgcttca
    21361 cctgaaagcc ctggaagcat gagatctaga aggatcaaat cgacaggata tgtgcttaac
    21421 aataattttc cttccgtacc tgaataagca tcaagaagtt tgtatctctg ttcaagaacc
    21481 tcctccagta aggaatgaac agcttcatcg tcttcgataa tcaaaatagt ttccagcagg
    21541 accacctact taatgtcaga tttttggaaa ataaacattc ctagagctga tgataccacg
    21601 attgttacta gactgaccag aatcattttc ggtgcttgcg aataagttgc ttgttgcgca
    21661 aagtccgtat acatgagcca cgtttccgta aaccactccg ttagaaattc caagcctttg
    21721 atttttgcga aaacaccggg aatgaaggcc gttcccatga tataaagaaa agaaagaagc
    21781 aaactgacac tggtacttct agtgatgaat gtcaaagcca ctggaacaga taatgtccct
    21841 aatgtcagca gaagcaatac aggaaatact tttagaacat aatttccata gtttttgatt
    21901 ccctcaactt caccaaatcc gaaagcaagt gtgtaagcaa gcatggctag tcccgtgatc
    21961 aaaaccacgc caagtgttgt gatcaaaagc gtaacgagaa gtttcgacac ataataagtc
    22021 attcgattcc gtcctaaaga taacgtattg cgaatcgtac cgttttgaaa ttcttcccca
    22081 atataaaagc acgcaaatgc tgcgaaagcc agaagaatcc atgcagccat tttttctacc
    22141 attaaaatcc ctgttgcacc gttgatgact gtatccgttg cttggtttaa ttcattgccg
    22201 tattttgtat gcatatacca ttcgttccaa gaataaaaaa gcggaagtac gaccatcagt
    22261 cctagtgaga cccacatttt acggctcttc catactttta ttttttcagc agttaataga
    22321 ttaaacatgt ggattgcctc cagttaattg taagaaataa tcttccagtt tttgcccaac
    22381 taggtgaata ccatccacag gaacttttgc ttctaccaat gcttgattga tggctgctac
    22441 ttggtcaatt ccttcaaaaa gctggataac acggctagat tgtacaaaat aatccctata
    22501 accaagagag tcgagaaccg ttactgcttg ttccgtcgcc gaagtgtcaa tttttataaa
    22561 ttgacgagat tcttgtgcca attcttcttt cgataattct ttgatcaagt gtccttcatg
    22621 caaaatacca tagtgagtgg cgatctgtga caattcatct agcaagtggc tagaaagcaa
    22681 gacggtgatg ccccgttctt ttaccaaacg ttggatgatt tctcgcattt ctgccatccc
    22741 tgctggatcc atcccattcg tcggttcatc caaaatcaaa aattctggat tcgtgatcag
    22801 tgcatttgca atagctaaac gctggcgcat acctaaagag aaattctttg ctttcttttt
    22861 ccctgtattt tccaatcgca tcattttcag caggtcttcg atttctctat ccgacacacc
    22921 gccgttcgct gcttggatac gtaggttatc tcttgcagtt aattctggat aaagagcagg
    22981 ggtttcaatc gattgaccca tacgtcgtct tgctgcctgc aaatccgtcg ttccaaataa
    23041 ttcgatcgaa ccttcatcaa tcgagatcaa acccataata gttctcataa aggtagactt
    23101 acccgctcca ttttccccaa tcaatccata gatcattcca cgtttgattt cgattgatac
    23161 tttatctaat gctttggcag caccgtattt tttggttata ccagttgctt tcaagaccgt
    23221 ttcatttcca aaatccatac ttatccttct ttcttattga tgagtgaagt atagttgacg
    23281 ggtataaaga aagcagccag aaatgtataa agaaagtgta aattttccaa ttgaatgtca
    23341 aaaaaaacaa tcggacaata cgtgtctttc ctattatcag gcagtttgtt atacgcttgt
    23401 atcagtttcc tgattctttc ttttcgacca aagtcgtttc tctgtattcc tcttcactga
    23461 aaatttttgc ttctttcaga tcaagcttga gtaattttac cagaccggtt ataaggataa
    23521 ctatagcaag actccctccc agaaaaatta gactgtctcg cagacgtggt tgcgttgtct
    23581 tccaatcaaa gagcgcacca aacagtgcta atccaagtgg actggccacc atggtaatcg
    23641 ttgttatcgt tgacataagt cgtccttgta tatgcgttgg aacttctcgc tgaagccaga
    23701 ccatataaaa agcaaaagaa aacattaatc cttaaaaacg gacttatgaa aaataataca
    23761 tgccgtttgg gatttacaat tagctaccaa ataagaggtt atgacaaaag acttttcaca
    23821 atcccttctt ccgaataaac ggtgttctcc tgccaactcc tcggtcacaa gtcacaatat
    23881 ttatcaaaca tgagaagcaa tttttgtaaa ttccttctta tttcttgaag ctgaccactt
    23941 ctgtcacaac ctcagaagcg gataaataat attgtttgat gatactttat ctttttcccg
    24001 tttgtttata gatcgaatga tattatagac caagaatcct acctattaac atagctaaaa
    24061 acacaaaaaa ggcactttcc aaattgtgga aagtgccaga aaagttagga ttaaggtaac
    24121 tgcgctcagc attgcttcgc ccagtacgat tctcgtaact cgtgaactcg aacgataatc
    24181 gcaattaacg ttttgagaac tgtgaagctt tacgggcttt cttaagacct ggttttttct
    24241 tcgtatcatt gaataagttt gaaaaacctt gatttataaa ggttttcccg taaatatatt
    24301 ttattatatt tgattaaatt tgaaatgttt gtgttctaaa atgtgttcta atttaaaggc
    24361 agtatatttc gtattttaga ttaaattttt aggctcattc attctcaaat ccattctatt
    24421 cagtcaataa aaattttaaa aagactttat aaacaaagat gttcaagcga gcatcttatt
    24481 tttttgtgct taatttgaaa ggagaacatt tcatgtataa aaaacaaaaa aagaaattat
    24541 gtgtattaac tattttgagt ttggctgttc aagcaattgt tggagcaatt gctcctacga
    24601 ttacttttgc tgatgaaatt acccatccac aaacggtaac cgtagaacta gatttagcac
    24661 atcaatatgc tgttgaagga acttttagtg atggtcgtcc aatgtcagaa gtcactgttc
    24721 cgcattatgc agtgtacaac ggctttaaac aagatatttt ctgtattgaa ccaggagtcc
    24781 cgatttacaa tgagttcact cctggctatg agaaaaaccc gttaccagat atgtccgata
    24841 aagccaaatt agtttccgtt ttatggaaaa aagtaggtac agatgcagat actcatattg
    24901 tggcacagaa gatgatctgg caagaagtta atgggtatac ccttcactca ataaaacgat
    24961 cagatggtag tgccgtaaat atcgcagcca ttgaggcaaa aataaatcaa gccattgacg
    25021 attatcagaa gaaaccaacc ttccataata gtacggctaa aacagtatta gggcaatcga
    25081 ctactgtgac ggatacgaat aatctgaatt tgtcagagtt cgatgaggtt gtggaaaatg
    25141 ccgcaaacat tgattatcgt gtgaatggta atcagttgat aatcacccca aatgccaatt
    25201 caaaagaaag tggtgtgctg acgcttaaga aatctgctgg taccggaaca cctgtcgcct
    25261 ataaaaaggc tggccaacag actttaatgg ccggggcgat tgataaacct aatacctaca
    25321 caatcaaaat tgatgtggaa acggagggtt ctttaaagat caaaaaagtc gataaagaat
    25381 cgggtgatgt tgtaccagga acggttttcc atttagactt tggaaaaact ttacctgcaa
    25441 aagacgtgac tactgacaaa gaaggcattg ctacattgga tgagattcct catggtacaa
    25501 aagtaaccat taccgaaaaa tcagtaccgg cgccttatac gattgatacc acccccatga
    25561 ctaccaccat taaagcaggc gaaaccattt acgtgacttc aaaaaacgca cgggaaaaag
    25621 ggcaaatcat tctggataaa agcggagttg aaacagggag tgatctttgg aatgacaatt
    25681 attccttagc tggtaatacg tttgccattc gcaaagacag tccaaccggt gaaatcgttc
    25741 aagaaatgac cacggatgaa aacggtcatg cggaaacacc aaaagagatt gctaatgcgt
    25801 tggaattggg aacctattat gtgaccgaaa ctaaagctag tcatgggttc gtgaatacgt
    25861 tcaaacctgt aaaagtcgaa ttgaagtatg ccaatcaaac cgtagcccct gttaccagta
    25921 acgtaaaagg gcaaaatcaa gaagtcactg gcgaaaccac tttgacgaaa gaagacaaag
    25981 atactgggga taaagcacaa ggtaaggcag tctttgaggg tgctgaatac accttgttta
    26041 ctgctaagga tggaaaagct gttaaatgga gtgaggcttt taaaccagag ttagcgaagg
    26101 gaacgaaagg ttctgatgaa acggtcactt tgactttaga cgaaaagaac caagttgccg
    26161 ttaaacattt agctatcaac gagtattatt ggcaagaaac aaaagcgccc gaaggatata
    26221 gccttgatga aacaaagtat cctgtatcca tcaaaaaggt ggatgatagc gaaaaaaatg
    26281 ccgtgattac ccgaaatgtt acggcaaaag aacaagtgat tcgctttggc tttgatttct
    26341 ttaaatttgc tggatcagct gcgggcacta ccgaaactgg cttcaatgat ctgaccttta
    26401 aagtgtcgcc attggaaggg accaatgaaa tcacaggtgc cgaagatgaa gcgaccacag
    26461 cttataacga gcaattaggg tttgatggtt atggcaagtt tgaaaatctt ccttatgggg
    26521 attatttact tgaagaagta gaagccccag aaggttttca aaagattacg ccattagaaa
    26581 tccgttctac ttttaaggaa aacaaagaag actttgtgaa gagtgagtat gtctttacca
    26641 ttaccgaaca agaccaaaaa caaccaatta aaacggtgac cgttccttac gagaagctga
    26701 cgaacaaagc attttctgtt agtttgaacc gtttaatgct ctatgatttg cctgaggaag
    26761 aagatagttt aacttctctt gcgacttgga aagacggaaa caaagaatta acttctctgg
    26821 attctaccga gctagttgat aaattgagtt acaatctaca tgaaatcaaa gaagactggt
    26881 atgtggtagc gcaagccatt gatgtggatg cgacaaaagc tgcccaagaa aaagatgaaa
    26941 aagccaaacc agtggtgatt gcagaaacaa gcgcaacgtt ggcgaacaaa gagaaaacag
    27001 gaacttggaa aattcagcat aaattaaccg ccgaacaagt tttaaataaa actattgtct
    27061 tattcaacta tgtgtatgaa aataaggaag cttttgaagc aggcgataag ccagtagcga
    27121 aggatgttag cttgaataac caagcccaaa ctgtcagttg tacagtggaa caccatgttt
    27181 ctatccaaac aaaggcccac ctagaaaacg gttctcagac ctttactcat ggtgatgtgg
    27241 tggatatgtt tgatgatgtg tcgattactc atgatgtact ggatggctca aaagaagctt
    27301 tcgaaacaat tctgtatgct ttattaccgg atggtacaaa caaagaaatt tggaaatctg
    27361 gcaagattga ttatgaagta aatgataaag aattcaccaa aactgtgctt gcgaaaaaag
    27421 tagataccgg aaaatatcca gaaggaacca agtttacttt tgcggaaatc aattacgata
    27481 aagatgggaa tatcaatgga aaacacaatg aagatttgaa agaaaaatct caaaccttaa
    27541 caccaaaaga agttccaacc atactaagta cgccgaaaca accggaaaca ccaactgttc
    27601 caagtggctc tcaagaatct agtcccacag tgaagacctt ccctcaaacc ggggagaaaa
    27661 attccaatgt tttactgttc attggtttta ctttgatttt tgcgacggcg ggctattatt
    27721 tctggaatcg ccggaactaa ggtgatgaga tgatacaagt aaatcctaag aaccaacgtg
    27781 cgcgaccgcc tcctaaaaaa gtagtcgcgc tatttttgta tccaaaacta acaatgaaaa
    27841 gaggaaaaga agatggaatt gaaatttgtc gtgccagata tggctgaaac ctttggaaag
    27901 ttaagttatg ccggtgaagg agaaattata acagaaggat atggacggaa tactacggtg
    27961 attggtcgca gttatcattt gtattccagt aagcaacggg ccgatgatat tgaggtggta
    28021 gtagctgcgg aagccggtga aaaggatttt gatcaagacc aaccgctaaa agccgtgaat
    28081 ccccatttgg ttgccaaagg ctatgagatt gagaatcgtg ggtttactga ttacgtgttg
    28141 tatgttgatg atttagtgaa agcataggag gaaaagaaga tgagattaac agaaggtatt
    28201 gtcgtagatt caggtttaac atttgggaag ttgcgttttt ccgcattacg ccgagaagta
    28261 cggaagcaaa atgaggatgg aacaattggt aatgaggtaa aagaacgaac ctataattta
    28321 aaatcctctg cacaagggcg aatgattcaa gttagtatcc cagctaatgt acccttacgc
    28381 gagtttgctt atgatgcaga ggtagagttg gtcaatccaa tcgtggatac cgttgccaat
    28441 tatgtttttc gagaaggaac taccgttaat tggttcatta aggcggatga tttagtcttg
    28501 aagcggcagc cgaatcaagg aaatcctacg aaccaaaatg aaagaaagaa ataggtgaag
    28561 attatggaaa ttaatgttga acatatttta gattgtctcg atcaatatgg aaaaggcgag
    28621 ctgactgagg aacaactcgt taaagcatta acctctgatg aaaaaatgtt tctgattata
    28681 catcaaggtt tacttggaga aaatagcgac agtgaagaag actctgatgt actagactgg
    28741 ttaggagaag aagagtcttt ctttatagtt gtcgaagtaa atgaaaactt atgtcggcag
    28801 gcagaatcca ttttggaaga aattggtgtg gagatgccgg atgctattga aggcttttta
    28861 aatcaattgg ttgaaacgaa acaactcccc tcctcgtagc agtaaatgac tagcataact
    28921 ttttgaaatt gcctaatgaa atcctattgg gctttttcga ttggaggtgg aaaccatcaa
    28981 aatgtacaaa ggacatcgca tacgcgcagg agatcagcac ttggtttatc actttgttct
    29041 tggctggcta ctcgctctgt tcatcggttg gatgagcgtc ttttattttc aagagtttag
    29101 acaatttgat atttctaagc tatcactttc tactattgaa atcgtttggt ctataaaaga
    29161 tttagtatgt ctgcttggaa gtctcgcttt ttcaggagct atgatattgc tttatattca
    29221 cttttttctt gatcattggc gaagcttgtg gcatcgacaa aagctagctc ggatgattct
    29281 agagaaccat tggtatgaag tgaaacaaac tcaaagtgaa ggctttttca aagatttgaa
    29341 tagtagccga accagagaga ctatcagtta cttccccaaa atctgttatc gaatgaaaga
    29401 tggattactg tctattcgcg ttcaaatttc acttgggaaa taccaagatc agcttttaaa
    29461 attggaaaag aagttagaaa gcggactgta ttgtgagtta gtagaaaagg aactcaaaga
    29521 ctcctatgtg gagtatactt tactgtatga tatgatcgcc aatcgaattg ggatagacga
    29581 agtagtggca gaaaatggga ctctgcgatt gatgaaaaat caagtatggg cttatgattc
    29641 tttaccccat atgttaatcg ctggtggcac gggtggaggg aagacctatt tcctcctcac
    29701 cattatcgaa gcactattga agtcagatgc agaattgttt atcctcgatc ccaaaaatgc
    29761 agatttagct gatttaggta cggtgatgcc tcatgtttat tcacaaaagg aagaaatttc
    29821 tgcttgtgtg gaagatttct atgaacgtat gatggctcgt agcaaggcaa tgaaagaaat
    29881 gtctaactac aaaacaggag agaattatgc ctatcttgga cttccaccca actttttaat
    29941 ctttgatgaa tacgtggctt atatggagat gttaacgacg aaagaaagtg cggtgatttt
    30001 gaataagctc aaacaaatcg tgatgttggg tcgtcaatct ggtttctttc tgattttggc
    30061 ttgccaaagg ccggatgcga aatatttagg agacggaatc cgcgatcaat tcaactttcg
    30121 cgtggctttg ggtcgtatga gtgaactcgg atattccatg atgtttgggg aggtcgaaaa
    30181 aaacttcttt atgaagcaca tcaaaggtcg aggttatgtg gatacaggtg gcagtgtgat
    30241 tagtgagttt tatacgccac ttgtaccaaa agggtatgat ttcttagatt caattagtaa
    30301 attagaaaag atgaatgatt gagtgatctt caatactgac tttatacttc aagtaacgta
    30361 taatactttt atatagtagg aagaaagggg ataattaata tggaaagtaa tgattcttat
    30421 ttagagtatg caaaagcaaa taaggataag tttatttctt atattatcaa agggaaatcc
    30481 cccgaagttg gacgagacgc agtttttatg gcagggagtc ctggggctgg aaaatcagag
    30541 gttgccattg gattagctga gaattatgat aaccatgtga ttattgatgc ggattacttt
    30601 cgcacccagt ttcctgatta taatggaaaa aactctagtc tctttcaaaa agcatcttct
    30661 tggttggtag agcaggcatt tagatatgtc ttagatcatg gctattcttt tattctggat
    30721 gcaacttttg caatattaag tgctgaaaaa aatatctcgc gggcgttgaa aaaacatttt
    30781 cgagtgacga ttttctatgt ttatcaagac cctaaaatcg cttggaaatt tacacaagaa
    30841 cgtgaaattg cagagggacg tcatgtgccg aaaaaggcat ttattaacgc ttttttcaaa
    30901 tcaagagaga acattgcaaa agtaaagagt aaacatccag atgtattgct tcatatcata
    30961 ataaaagatt atacgaataa tatttccgaa gttcattatg ctacggataa tctagaatta
    31021 tttttaccac tagggtatag taggaaagat ttggaggttc aattacatga ctgagaaaga
    31081 acaagtaacg aaaattgtaa agaaatacaa taagagcatt gcggatttat cggaaaatgc
    31141 tactgcaaaa gagttcaaaa cggtgattaa atatgtagcc gatcaggcca atgaaaaaca
    31201 acggaaatta gttggattaa ataaaaaata gtaggacaaa ttaataatgt atgagccaag
    31261 agcaaaatgt tcttggcttt tttctttgca atgaatgagg taatgtatga gatttttaga
    31321 tttattcgcc ggtattggtg gctttcgttt aggtatggaa caggcgagcc atcattgtat
    31381 cggtttttgt gaaattgata aatttgctag acgtagttat aaagcaattc atgatacaag
    31441 taaggaggtg gaaatgcatg acatcacaag tgtatcagat gagtttattc aatccctcgg
    31501 accagtggac atcctttgcg gcggatttcc gtgccaagct ttttcaattg cgggaaagcg
    31561 gcaaagattt tccgatactc gaggtacttt attctttgaa atcgctcggt tcaccactct
    31621 tctccgacct aagcttttat tccttgagaa cgtccgagga ttgctcaatc acgaaggagg
    31681 ggctacgttc gagacgatcc tccgaacgct ggatggattg gggtatgacg tggagtggta
    31741 ggtgcttaac tcaaaggcct acattcccca aagccgggag cggatattcc ttatcggaca
    31801 ttctcgagac gcatgtaccg aacaagtatt tcctatcatt ggaccgtctt caacatctga
    31861 tcaaaacatc agaaatttgg taaatatcaa tccttccaat cgaggaatgg gtggacaagt
    31921 ctatggttca gatggcgtag cgccaacctt aacaggtgat gaaggaatca aaatcgcact
    31981 gccagcaagg gatggaatct ctgtagcagg actattaccg ggaaagtttg aacaaggtaa
    32041 tcgagtttat gaagttactg gaaccgcccc caccttgtca acgaaacaag gtggtacaaa
    32101 aatcatgatt cgaaaaaatg aaacagaata tcaagaggtg aagccagaag acagtgtgaa
    32161 tctggctttt cctaattcca caagtcgccg aggtcgtctg ggaaaacaga gtgttcatac
    32221 cttactaaca ggagaccaac aagcgatcgt cacggatcag tatcaaattc ggaagctaac
    32281 acctagagag tgttggcgac ttcagggctt tcccgattgg gcttttgatc gagattctca
    32341 agtcaattca gatagtcagt tgtataagca agccggaaac tctgtaacag ttccggtcat
    32401 ttttgatatt gccagaagat taaaagagtt ggatgtacat ggtttatgat gagttgattg
    32461 gagaaattta ttgggtgatt ggaaagattc agtcagaccc agaattagaa gaacaattgc
    32521 gacggttaaa atttgatatt cgcaagaatg gtgtcaaagt cgctggcgat ccatatttaa
    32581 tgaatgagga gacggatgct cgaattgaaa taaatcaagt aattgcagaa tttgagcgga
    32641 ttgcggacca agcaaaagaa ccagatattc gtcaatactt gttagaaatg aaagcagagt
    32701 tggaagtaaa tggtattaca gatgagtaac ctaaaccaac acaactgact gttaggcgct
    32761 gaccgcggag gattcgcgaa gcagaaatcc tccgtggtgc tgtgcctgtt catggacggc
    32821 gttagccgac catgatgtct cacccccgta tctaacaggg gggtacaaaa acacaaaaaa
    32881 taagtaagca accattaaga atgcttgttg tatcaaagat agtaagtaat ttgtataggt
    32941 gttaactcat ttgaaaaagt taacaccttg aatttggagg tgagaacttg gcacaacgga
    33001 atttagatta tcggctatta aaagaccgac gcaacgaata cggcgtttca caaaataaat
    33061 tggctacggc ttgtggactt agtcggccgt acttaaatca gatagaaaat ggtggcgtaa
    33121 ccgcatctat aaaaaccatg aggaaaattt ttgaccaact ggaaagcttt aatcccgatt
    33181 tgcctttgac gttactgttt gactatgtaa ggattcgctt tcccacaacg gatgcacgaa
    33241 aaattattca agaaattctc catttgaaat ttgagtatat gctccatgaa gattatgcct
    33301 tttactccta tcaagaacag tatgtcatgg gtgatattgt ggtgatgttg tctcatgaag
    33361 aagataaagg tgtgctttta gaattaaagg ggcgaggttg ccgacaattt gaaacttttt
    33421 tactcgctca aaagcgtagc tggtacgact ttttcgagga ttgtctaaag gccggtggcg
    33481 tgatgaaacg cttggacttg gcaatcaatg atcgagtggg gctattggat atacctgagc
    33541 taacgaaaaa atgtcagaag gaagaatgta tctccttgtt tcgtaccttt aaaagttatc
    33601 gttccgggga acttttaaag gctgatgaaa aggacggcat gggcaatacc ttatatatcg
    33661 gcagtctcaa gagtgaggtt tatttctgtt tgtatgaaaa agactatgaa caatatatca
    33721 agttagggat tccattagac caaactgaga cgaaaaatcg ttttgagatt cgactgaaaa
    33781 atgaccgtgc ttttcatgcg attcaagatt tgttaaaagg tcgtagcatt gagagcacca
    33841 cgttttccat tatcaatcgt tacttgcgat ttgcggataa agtagaaggt aaaaggcgaa
    33901 ccaactggcc cttgaatgaa caatgggggc gttttattgg acggaatcgg aaagagattc
    33961 aattaacttc cgagccaaaa ccgtatacca ttgaacgaac cttgaattgg ctaggccgac
    34021 aagtcgcccc tacgtggaaa atggctaagg agttggatcg attgaatcgg acaacctata
    34081 tccaggatat ggtacagaat gcacgcttat cagaccgaca taaaaagatt ttggagcaac
    34141 aaagtatggc aattgaaaat ttgattatat gaaaagagga atcgtcatgg gaattgtaaa
    34201 agatattttt ttggtatttg gtggcagtat gcttggtgtt actgtgatgt gcttgatgaa
    34261 tgcaagtgca gcagcggatc gagcaatgga aaagaaagga aagtgacaag atgaatttcg
    34321 gacaaaattt
//
LOCUS       DACEEZ010000019        33736 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20313_2017
            SAMN12639966-rid9879223.denovo.019, whole genome shotgun sequence.
ACCESSION   DACEEZ010000019 DACEEZ010000000
VERSION     DACEEZ010000019.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639966
            Sequence Read Archive: SRR10023158
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 33736)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 33736)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741683.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741683.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:44:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,016
            CDSs (total)                      :: 2,962
            Genes (coding)                    :: 2,889
            CDSs (with protein)               :: 2,889
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 36 of 73
            Pseudo Genes (incomplete)         :: 29 of 73
            Pseudo Genes (internal stop)      :: 18 of 73
            Pseudo Genes (multiple problems)  :: 8 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..33736
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639966-rid9879223.denovo.019"
                     /isolate="E20313_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            214..498
                     /locus_tag="IWO89_001019"
     CDS             214..498
                     /locus_tag="IWO89_001019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1905 domain-containing protein"
                     /protein_id="HAQ0455790.1"
                     /translation="MREIYSFESKIYASEVDKGGAYIIFPYDIRKEFGAGRVKVQATF
                     DNMPYEGSIVNMGVKNPDGSVCYILGLRKDIRKNIGKEIGDIVAVTVKQK"
     gene            complement(779..1198)
                     /locus_tag="IWO89_001020"
     CDS             complement(779..1198)
                     /locus_tag="IWO89_001020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid decarboxylase"
                     /protein_id="HAQ0455791.1"
                     /translation="MEKETIVEVTKLGFTIQAAVKVIGKDLLITLTGGDTPHIGTVTT
                     FSRDTEMQTVRFPSHDGRFHKDDVLAEKIAVIIKTAVPGSCTITSGIHVDHISNAQIN
                     ASFPMSEELGYKILDWLEKIDFHIKEPVYYKDGEKTL"
     gene            complement(1200..1784)
                     /locus_tag="IWO89_001021"
     CDS             complement(1200..1784)
                     /locus_tag="IWO89_001021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UbiX family flavin prenyltransferase"
                     /protein_id="HAQ0455792.1"
                     /translation="MEQLAQRKKKIVIGVSGASGTIYAINLVKKLKEYPMIETHGIIS
                     PWAKQNLKLESDLSLTEFESLFDYHYSNKDLGAAVASGSFLTDGMIIVPASMKTVAGI
                     SVGLGDNLIARAADVTMKEQRKLVIVPRETPLNAIHLENLTKLARLGVQIIPPIPAFY
                     NQPQTIQDLVNHHTMKLLDQFGIVNEHSKRWQGD"
     gene            complement(1831..3306)
                     /locus_tag="IWO89_001022"
     CDS             complement(1831..3306)
                     /locus_tag="IWO89_001022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UbiD family decarboxylase"
                     /protein_id="HAQ0455793.1"
                     /translation="MTDQPYDLRKVIEELKTLPGQYHETDIEIDPEAELSGVYRYIGA
                     GGTVKRPTQEGPAMTFNNIKGFPGTRVNIGTMASRKRVGEILHHDYRTLGRFLKDAVE
                     NPIKPVKVDKADAPAQEVVHLATDADFDIRNILPAPTNTPEDAGPYITMGVVLGSDPE
                     KTMSDVTIHRMVLEDKDTIGMYIMPGGRHIGHFQKQFEALNKPMPITINIGLDPAIAI
                     GTTFEPPTTPLGYNELWCAGALRNEPIQIVDGIAVDEVGIARAEFIIEAEIMPNETMQ
                     EDINTNSGKAMPEFPGYTGDANPAVQVVKVKAVTHRKDNPIMQTTIGPSEEHVSMAGI
                     PTEASILALVDKAIPGKVVNVYNPPAGGGKLMTIMQIRKENEADEGIQRQAAILALSA
                     FKELKTVILVDEDVDIFDMNDVVWTFNTRFQGDKDIIVIPGMRNHPLDPSELPEYNPG
                     SIRFRGMSAKTIFDGTVPFDMKEHFVRAKFQEVEDWEKYLK"
     gene            3487..3798
                     /locus_tag="IWO89_001023"
     CDS             3487..3798
                     /locus_tag="IWO89_001023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0455794.1"
                     /translation="MKKMKESFPKAVKVENIANILKVTFENGEVKYVKSHWTEEITDA
                     LQFGKKGRGKRKNLLALSRNMWIGTEVTIEADGTVFINGKDRYTPEELWYKGKKSIPE
                     L"
     gene            complement(3913..6249)
                     /locus_tag="IWO89_001024"
     CDS             complement(3913..6249)
                     /locus_tag="IWO89_001024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAQ0455795.1"
                     /translation="MLQLKDIKKYYKVGETTTKALDGVSVAFRQKEFVAILGPSGSGK
                     TTMLNVIGGLDNYDSGDMVINGKSTKDFKDSDWDAYRNNSIGFIFQSYNLIGHLGIIE
                     NVELGMTLSGVSKDEKRKRAEDALHRVGLTDHMHKKPNQLSGGQMQRVAIARALANDP
                     DILLCDEPTGALDTETSIQIMELIQELSKEKLVIMVTHNPELAHQYADRIIEFSDGKI
                     LTDSHPHIERPKDDQFNLRRTKMSFWTALKLSFNNIRTKKGRTFLTSFASSIGIIGIA
                     IVLSLSSGFQKQIDNTQAETMAKFPITISKVTTNQTRDDAGLGASKADYPDSKTITAK
                     VSEEDKAQHTNKIDQTYVDYVTDIDPNLSNNIGFTRTTGINLLRDVNGKVQPVSFSNQ
                     NPDTESLSLSSTMSAMTGVGVSSFPTQLDTSKENFLKDNYSLLAGSYPASATDVVLIV
                     DGNNNTNINALKNLGFDVKEDEKLDFDDIVGTTFKLVNNNTYYTKLPTGNFIPNTDYD
                     AMYQNASDELKISGILRVKSSSTMNLLSPGIAYSDQLTTQIVNENKESEIVKAQRDSG
                     VNVLTTEKVDENAKQTLLSYLGGDSLPSSIMIYPNNFEDKEKILDYLDDYNKGKSDED
                     KIIYTDLAGTMTELTGGLMDAITYVLIAFAGISLVTSMIMISIITYTSVIERTKEIGV
                     LKALGARKKDITRVFDAETCILGISSGILGVFIAWLATFPINSILYNMTDLKNVAQLN
                     PVHAIILVIVSTILTMLGGHLPARMAAKKDAAIALRAE"
     gene            6391..7059
                     /locus_tag="IWO89_001025"
     CDS             6391..7059
                     /locus_tag="IWO89_001025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAQ0455796.1"
                     /translation="MNRILVVEDDPQLQLLYRSVLERAGFSVITALNGEDALKKLEAT
                     RIELIITDIMMPKMDGYDLLDSLRSTRFDTPVLMITAKADFEDKKRGFKLGADDYMTK
                     PIDVNEMILRVEALLRRSKINYSSSLIIGQTKLELDAYTVLQDQHSLILPQKEFLLLY
                     KLLSYPNKIFTRQQLMDDIWGLDTNTEERTIDVHIKRLRTRFEKTEDFQIITVRGLGY
                     KAVI"
     gene            7062..8105
                     /locus_tag="IWO89_001026"
     CDS             7062..8105
                     /locus_tag="IWO89_001026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HAQ0455797.1"
                     /translation="MKKKLFSQLWIYFASIVFISILVTLLCFLGFIFLLSRHSISPAE
                     SAGKPTLYPLFVFAGFSMVVGTGISIFVGRRILHPISALGTNMSLVATGDFSIRMDEQ
                     QKVAEVQQLYKDFNVMVQELNSIETLRNDFVSSVSHEFKTPLATIQGYVQLLQAPNLS
                     DEERQIFLYRIIESITQLSQLTENILKLNKLENQRIQLEKKEYRLDEQIREVIVFLQP
                     KWEKEQLELDIELAAVNYTGNEEFLYQVWLNIMDNAIKYNQINGQIHIKLFETATEIG
                     LEVTDSGVGMNEETRDRMFEKFYQGDTSRQISGNGLGLSLVKKILELHDGRIDYSSIE
                     GVGTTAMIRLSKQ"
     gene            complement(8342..9223)
                     /locus_tag="IWO89_001027"
     CDS             complement(8342..9223)
                     /locus_tag="IWO89_001027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HAQ0455798.1"
                     /translation="MDIHKFEVFLDLAETMNFTKTADRQFTTQGNISKQILSLETELD
                     VKLFERAHRKIELTEAGSLLLPYVKNVVEQYHAMQEVLETYTKDNNLSLNILTIPTMI
                     NYKGFSKITEFLKRHPEFTVQLKEVESVELTYNDANEHSDTIHFARNFQAPPSSIEWL
                     PTETDDFVAVLPKNHPLASKKKLDLSELKKENFLLLGPTTNLYQPVLDLCREAGFEPK
                     ISYKGARIDLIINMIANDMGIAIVMEKTVKNLLKENTVILPISPTTESYLAFTRKVGE
                     HSLASDTFWEYLNQEEN"
     gene            9382..10110
                     /locus_tag="IWO89_001028"
     CDS             9382..10110
                     /locus_tag="IWO89_001028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF554 domain-containing protein"
                     /protein_id="HAQ0455799.1"
                     /translation="MPTGVLINVGSVLLGGLIGGLVGNKLSEHFKAQLTMVFGVCSMG
                     MGIYSIAPMKNMPAVIFALVIGTAIGLIVHLGNGINKGAALMQVPISKIFPSEKLGMT
                     HDEFISTLVTVIVLFCASGTGIYGSLDSGMTGDSTILISKSVLDFFTAAIFACNLGYV
                     VSVIAIPQFIIFYILFLLAKFIYPLTTPDMILDFKACGGFLMVATGFRMINVKMFPVA
                     DMIPAMIVIMPLSWMWTNWIMPLL"
     gene            10266..10583
                     /locus_tag="IWO89_001029"
     CDS             10266..10583
                     /locus_tag="IWO89_001029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HAQ0455800.1"
                     /translation="MHIQIPTVLLDGTVLAALSKEDMYGYALTKIVQESLSVSESTLY
                     PVLRRLKKEGYLETYDEPFEGRNRRYYSLTPMGSRHLEEIIDEWIVFRDSLDRLLEES
                     TNE"
     gene            10576..11184
                     /locus_tag="IWO89_001030"
     CDS             10576..11184
                     /locus_tag="IWO89_001030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1700 domain-containing protein"
                     /protein_id="HAQ0455801.1"
                     /translation="MNEYLNEVVDHLQEIPEDDRFDLIQYYEEYFLDSGKTLDQIIDE
                     YGTPKQFALKLKISYFSEEEQAENELVKNPKRQIRLIWLIIIGLFASPVLIPLALGFI
                     LVIGGLLVALLALIFSIYVLCISILGVGLFMIISGISVFGQSILSGLFFIGVGLFATG
                     SAVFISPLLLKGTKGLFYVMMNFVKWMGRRFITKRDLHSAGV"
     gene            11200..12030
                     /locus_tag="IWO89_001031"
     CDS             11200..12030
                     /locus_tag="IWO89_001031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4097 family beta strand repeat protein"
                     /protein_id="HAQ0455802.1"
                     /translation="MRLKHFLITGIGLMLTGGLLAGATYTLGAQKSLTWDNGPRLIDM
                     VTETKKIKDANKIVIHAENQTVSVIRGLDFEIKTTYDKTEKPEIKEKNGVLTVNAVKK
                     STGAIISLEDTSAQITIVVPADHELSELTIEGNNSAIDINEVSIKKVNTKTHDGWVSF
                     NEFTGGTITAKDELGSLRLDEANVDNLIVTGENSNFHLEDSLISKEGKVILNGGNLSL
                     HEMENSGYTIQTSGESYIEKDYQPITNTLLEQGQKKLKITAKDTSINITTDQEDNETD
                     "
     gene            12168..12680
                     /locus_tag="IWO89_001032"
     CDS             12168..12680
                     /locus_tag="IWO89_001032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5348 domain-containing protein"
                     /protein_id="HAQ0455803.1"
                     /translation="MKVEEGQREKLKTEMERLHTYITQLSQTFYDPDKEKVMVNYPNN
                     SEGRQLEQVYHEVFKHLLTVKKELDYYSLPIIDTGILKYDGKKERFIFKSVREDLPLS
                     AGMDLEVLVEDYFTEEKHWVRTKLDYLPQAAGGTQARGWYITEDKELELEGVMARIRK
                     KIKKVTLDIY"
     gene            12706..13578
                     /locus_tag="IWO89_001033"
     CDS             12706..13578
                     /locus_tag="IWO89_001033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HAQ0455804.1"
                     /translation="MREKRAFYINGYAGIVGLVVLALVGLFFFYLGMWQAKVWALFLS
                     IFLWLITLLLLSSATVVSPNQAKVILFFGQYLGTIRENGFFLTIPLAQKMTVSLKVRN
                     FNSSVLKVNDLDGNPIEISAVVVFKVVDTAKALFDVAYYQDFVEIQSETAIRHIASQY
                     PYDTFNENDLTLRGNTTAVSDELQKELQERLAVAGVEVIETRLNHLAYATEIASAMLQ
                     RQQAKAILSARQTIVEGAVTITQMALEQIEDGQEINFTDDRKVQLINNLLVSIITDKG
                     TQPVINTGDMKDRK"
     gene            13754..14116
                     /locus_tag="IWO89_001034"
     CDS             13754..14116
                     /locus_tag="IWO89_001034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YxeA family protein"
                     /protein_id="HAQ0455805.1"
                     /translation="MKKLLIGLLVFIVVVLAGVKIADYVVMGGEEYYVQITTDGTKTV
                     SKSDSGQETVNYRYILPGYTKEGKEKELDFKGQLTRPLRKGAYLKITWNKNKGVTSYE
                     EVEKKDVPKTALEKLIKG"
     gene            14125..14877
                     /locus_tag="IWO89_001035"
     CDS             14125..14877
                     /locus_tag="IWO89_001035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ0455806.1"
                     /translation="MNKIVEVQNVTKVYGKNNEKKTQALSGISFDVEKGEFIGIMGAS
                     GSGKSTLLNILSTLDKPTDGHIRINQKDVTTLKGNQLADFRANEIGFIFQDFNLLESL
                     TAQENIAVPLSLQGVRPKEIKQRVQKIAERLSISHILESYPSEISGGQKQRVAAARAL
                     ITQPTILLGDEPTGALDSKSARDLLDTMDELNTKDHVSILLVTHDPFSASYCQRILFI
                     KDGGIHQEVKRGDQSRDSFYREILSILGNLEQ"
     gene            14891..16693
                     /locus_tag="IWO89_001036"
     CDS             14891..16693
                     /locus_tag="IWO89_001036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAQ0455807.1"
                     /translation="MLWKLSLTGIKSRLRDYLVLFSGLVMASAIFYMFESMASNEAFL
                     KSNTIVAATVIIFRFGSVLLGIITFVYILYANSFLMTMRQKDYAMFMMLGAKGRKIAQ
                     MIFSETFLVGIAATLVGSAVGVGLTSIVHRLLVDQLDLNISHFTPFSIHGLLITVLFF
                     AVLFLLAAVVNAFSIVKKPILALIRADQTPTRIKQHKFLFFLEVVLGIAFLGCGYYMM
                     KHVGTYQVFGIGVALVTIILGTYFIFHSVIIFFLSLLKKSEQISLKKLNNFTLSQLNF
                     RIREYTQMLSMVAMLFALALGALTVGLGFKNEIADLTENNSGYDLVMNNPRAEDQQKI
                     KELSPTLSASYTQKEDAGTIYYNASEFAAQPLIMIKHEDGTPPKITKEKVPAKKMNEL
                     TVQEELRSYELPEQQEKEIKVVSQSEFDQLNLPQSTLQLVQVKDFQANLKPIETLVAQ
                     NKMNNPSLQKVEYSNQKYEMYEVYNGLFSGFEFMGFFLGLAFLTMLASCLMFKILSGS
                     KSDIARYEMLEKIGTRRGLLKQSIRREVGVLFLAPGILGVVHVLFGLQMFGLFMQNPY
                     KDIWVPFTIFFVLYFIYYVLTTWLYTGIVLKKRL"
     gene            16970..18010
                     /locus_tag="IWO89_001037"
     CDS             16970..18010
                     /locus_tag="IWO89_001037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose mutarotase"
                     /protein_id="HAQ0455808.1"
                     /translation="MEINEQNFGENYHLITITNQNGTSLSVSDLGARVVHFQTIIDNE
                     KRELILGFDEAKEYLEKDPYIGASIGRMAGRITNGTFKLDDETFEVKTDPDTGHCLHG
                     GAPSFEAKQWHYTVINGENEASVIFYQTSPDMGNGFPGTLDVEVRYTLTNDDTWRVTV
                     QGISDRKTVFNPTNHVYFNLTGDPAQSIDEHILWLNSRLFATLNKDTTPTGEVVSVSG
                     TAFDFQRPKKLGEVFASDFRQSMIVGGIDHPFLLKKQSGLLNAAELTSPDEKVSIQVK
                     TDASGIVIFTANFGEDGPEMRGNKLVNHGGITFETQELPGAERLESFGDVTLEPNQVR
                     EFVTEYKINIKK"
     gene            complement(18062..20206)
                     /locus_tag="IWO89_001038"
     CDS             complement(18062..20206)
                     /locus_tag="IWO89_001038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HAQ0455809.1"
                     /translation="MKNKKTIRLLAATWLIAPSVLSTNTVFAVDNQIADQTVTTDTTT
                     SSSATSETTDSFTEDPTGTTNSTTGSTEPTEIDPADPSENEKPAETYSLTIAPSLIGK
                     IQLTASTADSPEQQPLDITESGTVEGLLAETKVTYTITPAEGEQIASVAVNGAKEVNY
                     DAKTFTVGTNNLSITAEFKAGPDTSTEKPTDPSEEVPSTPDPETPNKPDVGNNNNNNG
                     SNNSNNNNNNSNSGNSSNQTNPSGSNNNGNTQREPNHPNNGSSNGGTNNASSRPNTAN
                     QSGIENPSSASSDFVVKSPIDAVLPTNTTNVQQAIVREAFKHLGKPYVWGAKGPNTFD
                     CSGLTYYVYMKATGHYIGGWTGEQQYAGTQIPVSQAQPGDLVFWGPSSGVTHHVGIYI
                     GNGQFIHAPQPGDKVRVTSISDFTPDFAVRVNLAGLPAASGSLTGGSSILDGLDGSFH
                     FSQNQTTDQFLKKIADDAQEIGQKEGIYASVMMAQAILESGSGNSLLSSEPNHNLFGI
                     KGSYKGSSVTFNTLEQDSSGQSYQIRAQFRKYPSYKESLEDYADLIKNGLTGNPDFYK
                     PTWKSETKDYKEATKYLEGRYATDRQYSQKLNAIIEAYDLTKYDEPKKEAATTEEANA
                     EILNMSKRFDVPIKWTTDGVPITGLEFSTIKRVNNASSIFRVASIWDLWNNFTARQIP
                     ETTTRTVKAATVPVLTLLSLDKSFWLDLPLKK"
     gene            complement(20563..21936)
                     /locus_tag="IWO89_001039"
     CDS             complement(20563..21936)
                     /locus_tag="IWO89_001039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAQ0455810.1"
                     /translation="METTMNPEQMVLSEQIHMLIEQLGGKDNIDSVLPCATRLRIKLK
                     KQPVANAEKLKQQIPSIKYFILLDRYAEIIMDQGLAPAYKELTRQIDHSAPKEKLNPI
                     EKLFGLISEIFTPIIPLFAGSGILKGLVVLATTIGWLSETSGTYHILSAASNAVFYFL
                     PIFLAFSAAKTFKVNGYIAAVIAAALIEPNLTSLLSDSGKAATDFLGIPVVLMQYTST
                     VMPAILGIFFYSFVEKFLKKYIRENMQLVFVPLLSLVITVPLTLMVFGPAGVYLGEGL
                     AAAITWMMDINGPISGAIIAGGWNILVIFGIQWAVNPVMISNISAYGFDRIVPLTGAA
                     NFGMAGAALGVFLRSKRSKTRSISGSAFASILLAGVTEPTVYGIAIPLKKPFVAACIG
                     AAAGGAVMGFAQVKAIAFVFGSLTTLPAFISGTFFWYLAGLAVSLVVAMITTLVSGFD
                     EDLMSYE"
     gene            complement(21936..22331)
                     /gene="rbsD"
                     /locus_tag="IWO89_001040"
     CDS             complement(21936..22331)
                     /gene="rbsD"
                     /locus_tag="IWO89_001040"
                     /EC_number="5.4.99.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002829567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-ribose pyranase"
                     /protein_id="HAQ0455811.1"
                     /translation="MKKGKVINSDISRVIAQMGHFDKLSIGDAGMPVPFGTEKIDLAV
                     SNGLPSFLSVLENVLEELEVQSIELAEEIKTMNPEILEKIKLLLPDTPISFIPHEEMK
                     KELNNCHAFIRTGEMTPYANIILVSGVVF"
     gene            complement(22359..23276)
                     /gene="rbsK"
                     /locus_tag="IWO89_001041"
     CDS             complement(22359..23276)
                     /gene="rbsK"
                     /locus_tag="IWO89_001041"
                     /EC_number="2.7.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribokinase"
                     /protein_id="HAQ0455812.1"
                     /translation="MNTVTVIGSINMDTTLRLTNMPKPGETMHAHEIFHAGGGKGANQ
                     AVAAKRSGARTSFIGGVGADSEGQQLLDLLTKENIDTSGIAEIQGATTGQAMIMVDAA
                     GENSILIHAGANNAFHEQEVLKNKQLITNSDFIIAQFESSLDATILAFSIAKDAGKTT
                     ILNPAPARETIPTELLEKTDIIIPNETETEIITGIRVTDHNSLVAAAEKLHELGIGTV
                     IITLGSAGAFYHTEKEHGIVPAFKVDAVDTTAAGDTFIGALSSTLQPDLSNLKEAILY
                     GNLASSVAVQSYGAQPSIPYREALEQSAV"
     gene            complement(23384..24397)
                     /locus_tag="IWO89_001042"
     CDS             complement(23384..24397)
                     /locus_tag="IWO89_001042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family transcriptional regulator"
                     /protein_id="HAQ0455813.1"
                     /translation="MVKKKITIKEVAKLSGVSITTVSLILNGNTEKFSPKTVQKVLAA
                     KEELGYQPDYFARQMITKETKTIGVLVPDITNPFFSTLMRGIEDILYKQNFVTILCNA
                     DSDHQKEIEYLAELTRRGVDGFIIATSAVSTDAINENLKKQGRPFIVLDQKKSEGFSD
                     AVRTDDFRGGYLAGMHLLSLGHQTIALVYPENPPENVHARIEGFKSALDVYQIPHDQL
                     ILLPTQFSKQGGYQITAELLDSAATGVFALNDELAFGLYRGLEEAGKSIPEDYSIIGY
                     DNIDMCEYIKPKLTTIAQPIFELGQTSAKLLLDRIQFPEKEWEEKRLPVRLEKRFSTA
                     PLK"
     gene            complement(24478..25077)
                     /locus_tag="IWO89_001043"
     CDS             complement(24478..25077)
                     /locus_tag="IWO89_001043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HAQ0455814.1"
                     /translation="MNDLKENMIFDLSKTAFVAIDMQKEIVNNGTLSPHTAASILTNN
                     DLLVRTLKRTAALKVLVNVDISTFPYLSQQTDMGGMTASVPPEFTDLLLKDSLKDTDN
                     MLTIKKYNPSAFFGTSLDLQLRRRGIETIILSGVATTNGVYATALDAFQHGYHIVLAE
                     DACSDRDKESHQLFIKKIFPKTARVRSTKQIIEAIQQSK"
     gene            25263..25964
                     /locus_tag="IWO89_001044"
     CDS             25263..25964
                     /locus_tag="IWO89_001044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ0455815.1"
                     /translation="MKYVEVKNEYKRYKMGETTITANDDISFSIEKGELVIILGPSGA
                     GKSTILNILGGMDTPDEGQIIIDDTDIAQFSDKQLTAYRRTDVGFVFQFYNLVPNLTA
                     KENVELATEVSPDALDPVEVLRQVGLAHRLNNFPSQLSGGEQQRVSIARALAKNPKLL
                     LCDEPTGALDFETGKQVLKLLQNASRQHGNTVLIITHNSALAPIADRVIHINDAKVRS
                     VELNDHPSSIDEIVW"
     gene            25974..28685
                     /locus_tag="IWO89_001045"
     CDS             25974..28685
                     /locus_tag="IWO89_001045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HAQ0455816.1"
                     /translation="MKTRTYLKAGFREIRQSKGRFIAIILIVMLGTLLFVGVKTAGPV
                     MQKTMDHYVGTAGLSDLQIVSTGGLTQKDIAQAEKIPDAKVETGKQIYYSNTGKNEVI
                     QIFSYNKNNKQNKLQLTDGKLPEKPNQLVLDEKAEDEGYKIGDIYQIDSDELKEKEYT
                     ITGFVRSPLFINNLERGYANVGNGSVDYFVYLPESVFKTDIYSVLYLDFTNVKNLNTY
                     SKAYKDKMEQNQEKAEKYLQDRPEERLEELKKSANEELEPAQQKIADGKAQTTQARTR
                     LEDAKKQLAQQSATIQQLPEAQRTIAVQTLPQQEAQLAEQERQLVEKERELASAEEEL
                     NRNAEKIENLTKPSYLYNERSENVGFQEFGDLSDRIAAIANVFPVFFFFIAALITFTT
                     MTRMVEENRREIGTLKALGYTKVEIAKKYAIYASLASGIGIVLGTILGTNLLPRIIFE
                     LSNERYDIGSALIFYDWPPILQAAAAFFIAAFGAAMIVLFKDLRERPAALLQPKAPKP
                     GKRIFLEYITPLWSRLSFNQKISYRNLFRYKSRMLMAIIGIAGCAGLMVAGVGLKDSL
                     SSVSAKQFGPIIDYQAIVTLDSSTEDSRQKAADMLQKQEKVNEILAIHTQTVELRKKG
                     HASQSLTVIVPSKTADLDRFIHLTDLEENPRSLPKTGIAITQKVAELFDLSTGDHISL
                     YDEDQKEWKVEVKEIVKNYLGSFIYMSPEYYASVTDQSMMDNAFLLRTDKMTTKEEEM
                     LSEKLLATDAVTNTSFMSKQIETQEESMANLDSVVLIFVVLSGLLAFIVLYNLTNINI
                     SERVRELSTIKVLGFFDKEVTMYIVRENIIFTLLGIIGGFGVGYILTDFILQQASMEN
                     VIFPLVITWWAYVLSAGLTIVFTVIVMIVTHFKLKHIDMIDALKSNE"
     gene            29082..30710
                     /gene="treC"
                     /locus_tag="IWO89_001046"
     CDS             29082..30710
                     /gene="treC"
                     /locus_tag="IWO89_001046"
                     /EC_number="3.2.1.93"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-phosphotrehalase"
                     /protein_id="HAQ0455817.1"
                     /translation="MTFKDKVIYQIYPKSYKDSNGDGIGDLKGIKEKLPYLNDLGIDM
                     IWLNPIYPSPQKDNGYDISDYTAIDPIFGTMEEFEQLIAEAKKYRIEIMLDMVLNHVS
                     TEHEWFKKALAGDQHYQDFFILREEPTDWVSKFGGSAWSPFGDTGKYYLHLYDRTQAD
                     LNWRNQAVREELFKVVRFWMEKGVKGFRFDVINVIGKDEQLKNNKENDGKPEYTDKPI
                     THTYLKELNQATFGTDSTIITVGEMSSTTIENCILYTEPSREELSMVFNFHHLKVDYE
                     NGNKWSTPPFDFAELKRLFHTWGENMSEGNGWNALFFNNHDQPRALNRFVVIDQYRKQ
                     GAEMLATMIHLNRGTPYIYMGEEIGMIDPDYSSISDYIDVESLNAYKLLLEQGFTEKE
                     AFRRIKAKSRDNSRTPMQWSDTEQAGFTTGKPWLKVGGKLEEINVEKELSSEDSILSY
                     YKKLIRLRKTYPIVAQGDYHAYEANHPEVYGFLRQFEGQQLLVLTNFYDKDTRIAIPE
                     GFTEANILIDNYSDTVLHQSLELKPYQAIALLKN"
     gene            complement(30770..31951)
                     /locus_tag="IWO89_001047"
     CDS             complement(30770..31951)
                     /locus_tag="IWO89_001047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HAQ0455818.1"
                     /translation="MKKETDTTNRLIRFLGGKTSYYVLGIVILLALIIYFFHQISFIF
                     KPVGIIIATILPPLLFALILYYLLEPVIKRLSKHLSRNLSVIIVYVIILFLLGLGSIW
                     LIPFLEEQAKTLISSLPDLFQDFQESLRQFLEKTPFADSFNQFFASIDDVTSDIAGFI
                     GNYWESGAQRISNIFSTVTAIFITLFTGPIVAFFLLRDPQKFYQSVLAIVPPAFRTDF
                     KNLTKIANQQLGSFLKGQIIASFILGAVYWVCFLLIGLEFASVLAISAGLLCIIPYIG
                     PFIAFFPGLIIAFQDSTFMAVKFVIVWFAVQLLHGDLVIPRVMGDRLQIHPITILIVL
                     LVMGDLMGIVGVIFGIPIYTLVKLLVTFIFRKFKQRYNKFYGDKGEYENSSFSEEDYF
                     K"
     gene            32203..33726
                     /locus_tag="IWO89_001048"
     CDS             32203..33726
                     /locus_tag="IWO89_001048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein
                     AdcA"
                     /protein_id="HAQ0455819.1"
                     /translation="MKRLIGILAMLVLAGMLTACGASGKAEDSKEKLSVMTTFYPMYD
                     FTKAIVGDEGEVELLIPAGTDSHDYEPSAKDMAKIQDTDIFVYNDENMETWVPAIQKT
                     LQEGNVHTIKATEGMLLLPGSEEGHDHDHEHGEEGHTHELDPHVWLAPSLAIKQVANI
                     RDQLIEAYPEKQEVWTKNAAAYTEKLQALHQLYQETFKQAKQRSFVTQHAAFNYLALE
                     YGLNQVSIAGLSSSEEPSAARIAELKHFVKEHGINYIYFEENAKDSIARTLANEAGVS
                     LEVLNPLEGLTNEQIENGENYLSIMEANLEALKKTTETENPLEDTLTPKKEKSVYNGY
                     FEDEDVQDRSLSDWAGTWQTVDTYVEDGTFDPVFEYKEKLNQDKTAQEYKEYYTKGYQ
                     TDIASIKISEKEMAFTFKDGTTKRSNYRYVGKEILTYEAGNRGVRYLFEAEDKESGAF
                     RYIQFSDHTIAPADSEHFHLYAGNESQEVLLKEMENWPTFYPDTMDGREIAQEMLLH"
BASE COUNT        10690 a         6051 c         6863 g        10132 t
ORIGIN      
        1 aggaagaggt tgtgagcctg ccgtttaact ctgagtcaca aggagcaatt tcaaaaaaca
       61 gcttctcatg tttgttgact attgtgactt gtgaccgagg agttggcagg agaacaccgt
      121 ttattcgaaa aaaggaactg cgacaagact tttgtcacag ttccttcttt tttttgctat
      181 gctatactag atgtaaaaaa taggagaaat aaaatgaggg aaatttattc ttttgaatca
      241 aaaatctatg cttccgaagt cgacaagggc ggggcatata ttatttttcc gtatgatata
      301 agaaaagaat tcggtgctgg aagagttaaa gtccaagcaa cttttgacaa tatgccgtat
      361 gaaggcagta tcgtgaatat gggcgtgaaa aatccagatg gttcagtctg ctacatcctt
      421 ggactacgaa aagatattcg taaaaacatc ggaaaagaaa tcggtgatat tgtagcagtt
      481 acagtgaagc aaaaataaaa gaggttgtga caaaagacgt gtcacaacct cttgataaac
      541 ggtgttcaaa agccagtttc tcggtagtaa gtcaaattta tgcaaaaatg tggaaaccca
      601 tgtttacaaa atttttctta ctactcgaaa cgaagcggct ttatcacaac ctcttgataa
      661 acggcgttca atcagctgtc cctcggtatt agctcaaaaa cggcaaaata tgagaagcta
      721 tttttccatt tttttctctc atactcaggt cataacgctg atttcacaac ctcttctttc
      781 ataatgtttt ttcaccatct ttataataaa ctggttcttt gatatggaaa tctatttttt
      841 ccaaccaatc taaaatcttg taacctaact cttctgacat cggaaaagag gcgttgattt
      901 gtgcattgga gatatggtca acatggatac cggaagtgat cgtacaactt cccggaacgg
      961 cagttttgat gatcacagct attttttcgg ctaagacatc gtctttatgg aaccgaccat
     1021 cgtggctagg gaaacgtaca gtttgcatct ctgtatcacg agaaaatgta gtgactgttc
     1081 cgatatgtgg tgtatctccg cctgttaaag tgatcaataa gtctttaccg attactttaa
     1141 cagcagcttg gatcgtgaaa cctagtttgg ttacttcaac tatcgtttct ttttccatat
     1201 taatcaccct gccaacgttt tgaatgctca tttacaattc caaattgatc caaaagtttc
     1261 attgtatggt gattaacaag atcttggatc gtttgaggct gattataaaa agcaggaatt
     1321 ggtggtatga tttgcacacc tagtcgtgct aatttagtca agttttccaa gtgaatggca
     1381 tttaatggcg tttcacgagg aacaatgact aactttcgtt gctccttcat tgttacatca
     1441 gcagcacgtg caatcaggtt gtcgcctaaa ccaacagaga tacctgcgac tgttttcata
     1501 cttgccggta cgatgatcat tccatcggtc aaaaatgatc cgctggcgac agcagcacct
     1561 aaatctttgt ttgaataatg gtaatcaaac aggctttcga attcagttag tgataagtcg
     1621 gattccagct ttaagttctg ttttgcccat ggactgatga ttccgtgtgt ttctatcatc
     1681 ggatattctt ttagtttctt cactaggttg atggcataaa tggtgcctga agcgccactt
     1741 acaccaatca cgattttttt cttccgttgt gccaattgtt ccattttccg cactcctttt
     1801 tatgatgtca aaacagttgc agatcattca ttatttcaaa tatttttccc aatcttctac
     1861 ttcttggaat tttgcacgta caaaatgttc tttcatatcg aaaggaactg taccgtcaaa
     1921 gatagttttt gcactcatcc cacggaaacg aatagagcca ggattatatt ctggtaattc
     1981 tgatgggtcc aatggatggt tcctcattcc tgggatcacg atgatatctt tatctccttg
     2041 gaaacgagtg ttgaaagtcc aaactacatc gttcatgtcg aaaatatcaa catcttcatc
     2101 gactaaaatg actgtcttca attctttgaa ggctgataaa gcaagaatag ctgcttgacg
     2161 ctgaatacct tcatccgctt cgttttcttt acggatttgc atgatggtca tcaatttacc
     2221 accacctgca ggaggattgt agacatttac tacttttcct ggaatagctt tatcgaccaa
     2281 ggcaagaata cttgcttctg taggaatacc ggccatagat acgtgttctt cactgggacc
     2341 aatcgttgtt tgcatgattg gattgtcttt tcggtgagta actgctttga ctttcactac
     2401 ttgtacagct ggattagcat ccccagtata gccaggaaat tctggcattg cttttcctga
     2461 gttcgtgttg atatcttctt gcattgtttc atttggcatg atttctgctt caatgatgaa
     2521 ttcagcacga gcaataccca cttcatcgac tgctatacca tcaacgattt gaataggttc
     2581 gttacgcaaa gctcctgcac accatagttc attgtagcca agtggagtgg taggtggttc
     2641 gaaagtcgtg ccaatggcaa ttgctggatc taaaccaata ttgatagtga taggcattgg
     2701 tttattcaag gcttcgaatt gtttttggaa gtgaccaatg tggcgtccgc caggcatgat
     2761 gtacataccg atcgtatctt tatcttctag aaccatacga tggatcgtta catcgctcat
     2821 tgttttttct ggatctgatc ctaagactac acccattgtg atgtatggac cagcatcttc
     2881 aggagtattt gtaggtgcag gcaaaatgtt gcgaatatcg aagtctgcat cggtagctag
     2941 atgaacgact tcttgtgctg gagcgtctgc tttgtctact ttcacaggtt tgattggatt
     3001 ttctacagcg tctttcaaga aacgaccaag tgttcgataa tcatggtgca aaatttcacc
     3061 tacacgttta cgactagcca ttgttccgat gttcacacgt gtgccgggga aacctttgat
     3121 attgttgaaa gtcatagctg gtccttcttg agtcggacgt tttactgtac cgccagcacc
     3181 gatataacgg taaacaccag ataattctgc ttcaggatcg atttcaatat ctgtttcgtg
     3241 atattgacct ggaagtgttt ttagttcttc gatgactttt cttaaatcat acggttgatc
     3301 tgtcatgaga gattcctcct tagttgtctt tacctatcta tagtaaaagg tgaaaaagag
     3361 gaaaacaatt caacttgttc ggtaaagtat tctacaatgg aatggatgcg tttttttgat
     3421 aatagaataa actatgatat acttatcact aaaagaaagc gcatacttag tgttggagga
     3481 aggaaagtga aaaaaatgaa ggagtcattt cctaaagcag taaaagtgga aaatatagcg
     3541 aatattttga aagttacatt tgaaaacgga gaagtaaaat acgtgaaatc tcattggaca
     3601 gaagagataa ccgatgcctt gcaatttgga aaaaaaggaa gaggaaaaag aaaaaatctt
     3661 ctcgcacttt ctcgtaatat gtggattggg actgaagtaa cgattgaagc agatggaaca
     3721 gtattcataa atggaaaaga caggtatacg ccagaagagt tgtggtacaa aggaaagaaa
     3781 agtataccag aattataaaa aataattaaa aaaaacagcg cgaaacaagg aacatattac
     3841 ttcctcgttt cttgctgttt tctactattt cagcgctctt atgactcaaa tctaaatgtg
     3901 gttgtcacaa ccttattctg cacgcaaagc gatagcggcg tcttttttcg ctgccattcg
     3961 tgcaggaaga tgaccgccaa gcatcgtcaa tatagtagag acgatcacta gaataatcgc
     4021 atggacagga tttagttgag caacattttt tagatccgtc atgttgtata agatgctatt
     4081 gatagggaac gtagctagcc atgcaataaa cacacctaat atcccagaag aaatacctaa
     4141 aatacacgtt tctgcgtcaa atacgcgagt gatgtctttt ttacgtgcac ctaaagcttt
     4201 tagtacaccg atttcttttg tccgttcaat cacagatgta taagtaatga tactgatcat
     4261 gatcatactt gttactaaag agatccctgc aaaagcaata aggacataag tgatagcatc
     4321 catcaatccg ccagtcagtt cagtcatcgt tccagctaaa tcagtataaa tgattttatc
     4381 ttcatctgat ttgcctttat tgtaatcatc tagataatcc aagatctttt ctttgtcttc
     4441 aaaattgttc ggataaatca tgatacttga aggcaagctg tctccaccta aataactaag
     4501 caatgtttgt ttggcatttt cgtcaacttt ttcagtagtt aagacattta caccactgtc
     4561 tctttgagct ttgacgattt ctgattcttt gttttcgttg acgatttgag tcgttaattg
     4621 atcgctataa gcgattcctg gagataagag attcattgta gaagaagatt tcacgcgtag
     4681 gatccccgag atcttcaatt catcagaagc attctgatac atcgcatcat aatctgtatt
     4741 agggataaaa ttcccagttg gaagttttgt atagtaagta ttgttgttta ctaatttgaa
     4801 tgttgtacca acgatatcat caaaatcaag tttttcgtct tctttcacat caaatccaag
     4861 attcttcaat gcgttgatat tagtattatt attgccgtct acgatcagca cgacatctgt
     4921 tgctgaggct ggataacttc cggcaagtaa agaataattg tcttttaaaa agttttcttt
     4981 gctagtgtca agctgtgtag ggaaacttga cactcccaca cctgtcattg ctgacatcgt
     5041 actagataaa gaaagagatt ccgtatcagg attttgattc gagaagctga caggctgtac
     5101 tttgccattt acatcacgta aaagattgat tcctgtagta cgggtaaatc cgatattatt
     5161 acttaagtta ggatcgatgt ctgttacgta atcgacgtat gtttgatcaa tcttattcgt
     5221 gtgctgtgct ttatcttctt cgctgacttt tgcagtaatc gttttgctgt ctggataatc
     5281 cgctttgctt gcacccaatc cggcatcgtc acgagtctgg ttagtcgtta ccttagaaat
     5341 agtaatcgga aatttcgcca tcgtttcagc ttgcgtgtta tcgatttgtt tttggaatcc
     5401 agaagaaagg gacagaacga tcgcgattcc gatgatccca atactggaag caaaggaggt
     5461 caagaaagta cgtccttttt ttgttcggat attgttgaag gaaagcttta acgctgtcca
     5521 aaaactcatc tttgttctgc gtaagttaaa ttgatcatct tttggtcgtt cgatgtgagg
     5581 atgggaatct gttaagatct taccatcaga aaattcaatg atccgatctg cgtattgatg
     5641 ggccagttca ggattatgtg tgaccatgat cactaatttt tctttcgata actcttggat
     5701 caattccatg atctggatac ttgtttctgt atctaacgct ccggttggtt catcgcacaa
     5761 aaggatatct gggtcattgg ctagtgctcg agcgatggcc actcgctgca tttgtccacc
     5821 agataactga tttggctttt tatgcatatg atctgtcaag ccgactcggt gcaatgcatc
     5881 ttctgcccgt tttcgttttt cgtctttaga aacaccactt aatgtcatcc caagttcaac
     5941 gttttcaata atgcctagat gaccaatcaa gttgtaactt tgaaaaatga aaccgataga
     6001 gttgtttcgg taggcatccc agtcactgtc cttaaagtct ttagtagact ttccgttgat
     6061 taccatatca ccggaatcat aattatctag tccaccaatc acattcagca tagtcgtttt
     6121 accagaacca ctaggaccta aaatcgctac gaattctttt tgtctgaaag cgacagacac
     6181 accatccaat gctttggtgg tggtttcgcc gactttataa tattttttta tatcttttaa
     6241 ttgaagcata atcacccttc tcccgtattg aaatattcgg tatactaata agtatatcag
     6301 aacaatgatc gtagtatacg aaataaatgt taactgaaga tgaactacaa gaaaaaatct
     6361 agaaattttt caaaaaacag agggagatca atgaatcgta ttttagtagt agaagatgat
     6421 ccgcaattac agttgcttta ccgttctgtg ttggaacgag cgggcttttc agtcatcacc
     6481 gcattgaatg gagaggatgc gctgaaaaaa ctagaagcga cacgtataga attaatcatt
     6541 acagatatca tgatgccgaa aatggatggc tatgacttgc tagactcatt acgtagcacc
     6601 agatttgaca cacctgttct aatgattacg gcaaaggccg attttgagga taagaaacgc
     6661 gggttcaaat taggagcaga tgattatatg acaaaaccca tcgatgtcaa tgagatgatc
     6721 ctacgtgtgg aagcattgct tcgacgttca aagatcaatt acagcagtag cttgataatc
     6781 ggacaaacaa aattagagct agatgcttat acagtgttac aagatcagca ttcgttgatt
     6841 ctgcctcaaa aagaatttct gcttctgtat aaattactgt cttatcccaa caagatcttc
     6901 actcgccaac agttgatgga tgatatttgg ggattagata cgaatacaga agaacgaacg
     6961 atcgatgtac atatcaaacg tttaaggacc cgttttgaaa agacggaaga ttttcaaatc
     7021 atcacagtac ggggactagg ctataaggcg gttatttaaa gatgaaaaaa aagctatttt
     7081 cacaattatg gatctatttt gctagtattg tcttcatttc gatcttagtg acactgctgt
     7141 gttttctcgg ttttattttc ttattgtctc gacattctat ctcgcctgct gaatcggctg
     7201 gaaaaccaac gttatacccg ttattcgtct ttgcggggtt cagtatggtc gtgggtacag
     7261 gaatatcgat ttttgttggg cggcgtatcc tgcatccaat cagtgcgcta gggacaaata
     7321 tgagtctggt agcaacggga gatttttcga ttcgaatgga tgaacagcag aaagtagcag
     7381 aagtacagca actgtataaa gatttcaacg ttatggtcca agaactcaat agtatcgaga
     7441 cactgaggaa tgactttgtg tccagtgtat cccatgaatt caaaacacca ctagcaacaa
     7501 tccaaggata tgttcaattg ttacaagccc caaatctctc tgacgaagaa cgccaaatat
     7561 ttttatatcg gattatcgaa agtatcacgc agctttcaca attaacagag aacatcttaa
     7621 aattgaacaa actggaaaac cagcgtatcc aactggaaaa gaaagagtat cgattagatg
     7681 aacaaatcag agaagtaatc gtttttttgc agcctaaatg ggagaaagaa cagctagaat
     7741 tggatatcga acttgctgca gtaaattaca caggcaatga agagtttttg taccaagtgt
     7801 ggctgaatat catggataac gccataaagt acaatcaaat caatggacag attcatatca
     7861 aactatttga gacagccaca gagattggac tggaagtgac cgattcaggt gtaggaatga
     7921 atgaagagac cagagatcga atgtttgaaa aattttatca aggagataca agtcgtcaaa
     7981 tttctgggaa tgggctagga ctttcactgg ttaaaaaaat tctagaactg catgatggac
     8041 gtattgatta tagcagtatc gaaggagtag gaacgacagc aatgatacgg ttaagtaaac
     8101 aataggaaaa agaataaaac ccctaaaaat tctattgaag gtttttaggg gtttttagtc
     8161 gtttttttgt gattagggaa atgttcttac ggttcttttg gcataaaaat cgctgctttc
     8221 ttattgaact acgagaaatg ttagaggtgt cattgaaaca aggacatgat gcaagaaaga
     8281 gagcgaaagt catgcgtcgc tctctttttt tgggtggata gatggacaga aaatgtaggg
     8341 cttagttttc ttcttgattt aaatactccc aaaatgtatc tgaagccaaa gaatgctcac
     8401 cgactttacg agtaaatgca agataactct ctgttgttgg agaaataggg agaatcacag
     8461 tattttcttt taatagattc ttgactgttt tttccataac aatagcaatc cccatgtcat
     8521 ttgcaatcat attgatgatc aagtcgattc ttgctccttt ataagagatt tttggttcaa
     8581 atccagcttc gcgacacaga tctagtacag gttgatacaa gttcgtagtg gggccaagga
     8641 gcaaaaagtt ctcctttttc agttcgctta gatccaattt ctttttacta gctaaaggat
     8701 gatttttagg tagaactgct acaaaatcgt ctgtttcagt aggcaaccac tcaatcgaac
     8761 ttggtggggc ctggaaattt ctagcaaagt ggatcgtgtc actatgttca ttggcgtcat
     8821 tgtaagttaa ttcgacactt tccacttctt tcaactggac tgtaaattca ggatggcgtt
     8881 tcaaaaactc ggtgattttg gaaaaacctt tataattgat cattgtcggt atcgttaaga
     8941 tatttaatga aagattgttg tctttcgtat aggtttcaag cacttcttgc atagcatggt
     9001 attgttctac gacatttttt acatatggga gcaataagct tccagcttcg gtcaattcga
     9061 tttttctatg ggcgcgttcg aataatttga cgtctaattc tgtttctaac gaaagaatct
     9121 gtttagaaat atttccttgg gtggtgaatt gtcgatcagc agttttggtg aaattcattg
     9181 tttcggctaa atcaagaaaa acttcgaatt tatggatatc catcatattc ctcctactta
     9241 ttccaataaa gaatacttta acacaaaaaa tgaattggtg taatcttctc tgtctgttac
     9301 aatctttctt gcagcgaaag caataattac agaaattttt ctgatttttt tcgcttcgta
     9361 gaaaaagaga ggagtttttt gatgccaact ggagttttga tcaatgtagg ttccgttttg
     9421 cttggcggac taattggcgg attagtagga aataaattaa gtgaacattt taaagcacag
     9481 ctgacgatgg tttttggtgt gtgttcgatg gggatgggaa tctattccat tgcgccgatg
     9541 aaaaatatgc cggctgttat ttttgcttta gtgataggaa cagcgattgg tctgattgtt
     9601 catttaggta atgggataaa taagggggct gcgctgatgc aagtacctat ttctaaaatc
     9661 ttccctagtg aaaagcttgg tatgacccac gatgaattta tcagcacatt agtgacggtc
     9721 attgttctat tttgtgcaag tggtacgggg atctatggaa gtttagattc tgggatgaca
     9781 ggagacagta cgattttgat ttctaaatct gtccttgatt ttttcacagc agctattttt
     9841 gcgtgtaatt tagggtatgt cgtttctgtg atagcaattc cccaattcat tattttttat
     9901 attttgttct tattggcaaa atttatttat ccattgacta caccagacat gatcttagat
     9961 tttaaagctt gcggcggatt cttgatggtc gctaccggtt ttagaatgat caacgtgaaa
    10021 atgttcccag tagctgacat gatacctgca atgatcgtta tcatgccact gagctggatg
    10081 tggacaaatt ggattatgcc actgttataa taaaaatcaa agagaagcca ttttttgctg
    10141 actaaaaagg atcaaatctt ttagtcagtt tttttgtatt tgacaaactg attttgaaac
    10201 tatattatat gttgtataat atagattgaa ataaaatatt acacttgaga atagaaggtg
    10261 acgtaatgca catccagatt ccaactgttc ttcttgatgg tactgtgcta gcagcattaa
    10321 gcaaggaaga tatgtatggg tatgccttaa caaaaatcgt acaagaaagt ctctctgtca
    10381 gtgaatctac actatatccg gttttgagaa gattgaaaaa agaaggatat cttgagacct
    10441 atgatgaacc ttttgaaggc agaaaccgtc gatattactc gttgacacca atgggaagtc
    10501 gtcatttaga agaaattatt gatgaatgga ttgtttttcg agattcttta gaccggttat
    10561 tggaggagag cacaaatgaa tgaatatttg aatgaagttg tggatcatct tcaagaaatt
    10621 cctgaagacg atcgttttga tttgatccag tattatgagg aatatttttt agattctgga
    10681 aaaacattag atcagatcat tgatgaatat ggtacaccaa agcagtttgc cttaaaatta
    10741 aaaatcagtt acttttcaga agaagaacaa gcagaaaacg agttagtgaa aaatccaaaa
    10801 agacaaattc gcctgatttg gttaatcatt atcggtttat ttgcttctcc tgtcctcatt
    10861 ccactagcac tggggtttat attggttatt gggggactat tggttgcttt actagcgctc
    10921 atttttagta tctatgtttt atgtatcagt atcttaggtg ttggcctatt catgatcatc
    10981 tcaggaatca gtgtgtttgg acaatcgatt ctgagtggat tattctttat cggtgtcggt
    11041 ctgttcgcca caggaagcgc agtatttatt tcgccactgt tgctgaaagg aacaaaaggg
    11101 cttttttatg tcatgatgaa ctttgttaaa tggatgggac gacgttttat tacaaaaaga
    11161 gatctgcatt cagcgggcgt ttgaaggagg acacataaat tgagattgaa acattttttg
    11221 attaccggta ttggtctgat gcttactggt ggcctcctag caggtgcaac ttatacatta
    11281 ggcgctcaaa agagtctgac ttgggataat ggtcctcggt tgatcgatat ggttactgag
    11341 acaaaaaaaa tcaaggatgc aaacaaaatc gtcatccatg cagaaaatca aacagtttcg
    11401 gtcatacgcg gattggattt tgagataaaa acgacttatg ataagacaga aaagccagaa
    11461 ataaaggaga aaaatggcgt gttgactgtg aatgcagtca aaaaatcaac aggtgcaatc
    11521 attagtttag aagacacaag tgcacaaatc actattgttg tccctgcaga tcatgaatta
    11581 tctgaattga caatcgaagg gaataatagt gcaattgata tcaacgaagt atcaatcaaa
    11641 aaagtgaata ctaagacaca tgatggctgg gtatcgttca atgaatttac aggaggaaca
    11701 ataactgcga aagatgaatt aggctcactt cgattagatg aggccaatgt ggataacttg
    11761 atcgtcacag gtgaaaacag caacttccat ctagaagaca gtttgatcag caaagaagga
    11821 aaagtcatcc taaacggtgg caatttatct ttgcatgaga tggaaaacag tggctatact
    11881 atccaaacgt ctggtgaatc ttatattgaa aaagactacc agccaataac aaatactttg
    11941 ttagaacaag gacagaaaaa gctaaagatc actgctaaag acacatcgat caatattacg
    12001 acggatcaag aggacaatga aacagattga tcctgtactc aactggatat tttaagaaaa
    12061 aacagcaaag atccttccaa tctttgctgt ttttcatcgc ttaacgcaca taactatagt
    12121 aaaataagaa agaaaatatc taaaaagaaa acagggtgaa agagtatatg aaggtagaag
    12181 aaggtcaaag agaaaagtta aaaacagaaa tggaaagatt acatacttat atcactcaac
    12241 taagccaaac cttctatgat ccagacaaag aaaaagtgat ggttaattac ccgaacaatt
    12301 cagaaggtcg ccaattagag caagtgtacc atgaagtttt caaacattta ttgacggtga
    12361 agaaagagct ggattattat tctttaccga ttatcgatac aggaatattg aagtatgatg
    12421 ggaagaagga acgatttatc tttaaatctg ttcgggaaga tttgcctctt tcagcaggga
    12481 tggatctaga ggtcttggta gaagactatt ttacagaaga aaagcactgg gttcgaacaa
    12541 aattggatta tttaccgcag gcagccggag ggacacaggc aaggggttgg tacatcactg
    12601 aagacaaaga gttagaacta gaaggtgtaa tggcgcgtat tcgaaaaaaa attaaaaaag
    12661 taacattaga tatttattga aggataaagg agcgcaggaa gtagcatgag agaaaaaaga
    12721 gcattttata tcaatggtta tgcagggatt gtcggattgg tcgtattagc tttagttgga
    12781 ttgtttttct tctatttagg tatgtggcaa gcgaaagttt gggcactttt tttaagtatt
    12841 tttctttggt tgatcacgtt attgttgtta agttcggcaa ctgtagtcag tcctaatcaa
    12901 gcaaaagtaa ttttgttttt tggacaatat ttaggtacga ttcgagaaaa tggctttttc
    12961 cttacgatac ctttggcaca aaaaatgact gtttcgttga aagttcggaa tttcaatagt
    13021 tctgttttaa aagtcaatga tttggatgga aaccccatcg aaatctcagc agtagtagtc
    13081 tttaaagtcg ttgatacagc aaaggcatta ttcgatgtag cgtattatca agattttgta
    13141 gaaatccaaa gtgagacagc cattcggcat atcgctagcc aatacccgta tgatacattc
    13201 aatgagaatg atttgacgct tcgaggaaac acgacggcag tgtctgacga gttgcaaaaa
    13261 gagctgcaag agcgtttagc agtagcaggt gtcgaagtaa tcgaaacacg cttgaaccat
    13321 ttagcctatg caacagaaat cgctagtgcc atgctccaac gtcaacaagc aaaagccatt
    13381 ttgtctgctc gacaaaccat tgttgaagga gctgtcacaa tcactcaaat ggccttagag
    13441 caaatcgaag atggacaaga aatcaatttc accgatgatc ggaaagtcca attgatcaat
    13501 aatttacttg tctcaatcat tacagacaaa ggaacgcaac ctgttatcaa tacaggcgac
    13561 atgaaagacc gtaagtgaca tactttttgt aagataggaa gaaggagttt aggacggatt
    13621 attgtcttga actctttttt tagttgtttt aaagaaaatg acaaaattga aagaagaatg
    13681 atagttgatt cgaaggcaga attgttcgct tttttctata atcagtatat aaagtaagag
    13741 agaaggggaa aaagtgaaaa aacttctgat cggtctactt gttttcatcg ttgtggtact
    13801 tgcaggggtg aaaatcgcag attacgtagt tatgggtgga gaagaatact atgtacaaat
    13861 tacgacagat ggtacaaaaa cagtttcaaa atcagactct ggacaagaaa cagtcaacta
    13921 tagatatatc ctgcctggct atacgaaaga gggtaaggaa aaagaactgg acttcaaggg
    13981 acaattgact cgaccgcttc gtaaaggagc atatttaaaa atcacctgga acaaaaataa
    14041 aggtgtcact tcgtatgaag aagtcgaaaa aaaagacgtg ccgaaaacag cactggaaaa
    14101 actaattaaa gggtagggaa agaaatgaac aaaatagtcg aagtacagaa tgtaacaaaa
    14161 gtatacggaa aaaacaatga gaaaaagacg caagcattaa gcggcatcag cttcgatgta
    14221 gaaaaaggcg aatttattgg gatcatgggc gcttctggtt ctggtaagtc gacacttttg
    14281 aatattttat ccacattaga taaaccaaca gatgggcata tccgaatcaa tcaaaaagat
    14341 gtgaccacat taaaaggaaa tcaactagca gattttcgtg cgaatgagat tggctttatt
    14401 ttccaagatt tcaatttatt agaaagcttg acagcacaag aaaatatcgc tgttcctctt
    14461 tctttacaag gcgtcagacc aaaagaaatc aagcaacgtg tccaaaaaat tgcagaacgt
    14521 ttatccatct ctcatatttt ggaaagctat ccttctgaga tttccggtgg acaaaaacaa
    14581 cgggtcgccg ccgcacgtgc attgatcaca caaccaacga ttctattagg ggatgagcct
    14641 acaggtgcgc tggattccaa aagtgcccgg gatcttttag atacgatgga cgaattgaat
    14701 acaaaagatc atgtatcgat tcttcttgtg acgcatgatc cgttttctgc aagttattgc
    14761 caacgtattt tattcatcaa ggatggcggt atccatcaag aagtgaaacg tggagatcaa
    14821 agccgtgatt ccttctatcg agaaatacta tctattcttg gaaacttgga acagtagggg
    14881 gagattgact atgctttgga aattatcact tactgggatc aagagccgtt taagagacta
    14941 cttggtgtta ttctcaggat tagttatggc atctgctatt ttttacatgt ttgaatcaat
    15001 ggcaagcaac gaagcttttt tgaaaagtaa tacgatcgtt gcggctacag ttatcatttt
    15061 ccgattcggt tctgttttac tagggatcat cacattcgtt tatattttat atgcaaattc
    15121 ctttttgatg acgatgcggc aaaaagacta tgcaatgttt atgatgctgg gagcaaaagg
    15181 cagaaaaatc gcacagatga ttttttctga aacattctta gtagggatcg cagctacttt
    15241 agtgggttca gctgtcggtg taggattgac aagtatcgtc caccgattat tagtggatca
    15301 gttagatctc aatatttcgc attttacacc ttttagtatt cacgggttgt tgatcactgt
    15361 tttgtttttt gctgttttgt ttttattagc tgctgttgtc aatgcttttt cgattgtcaa
    15421 aaaaccgatt ttagcattga ttcgtgcgga tcagacacca acaagaatca agcagcataa
    15481 attcttgttt ttccttgaag tagtactggg aattgctttt ctaggctgcg gttactatat
    15541 gatgaaacac gtggggacct atcaagtctt tggtataggg gtagccttag ttacgatcat
    15601 attaggaact tatttcattt tccattctgt gatcattttc tttttatcat tactaaagaa
    15661 atcagaacag atttcattga aaaaattgaa caatttcaca ttgtcacagt taaactttcg
    15721 tattcgtgaa tatacacaaa tgctctcaat ggtcgctatg ctgtttgcct tggcactggg
    15781 cgctttgacg gtaggattag gttttaaaaa tgaaattgca gacttgacag aaaacaattc
    15841 tggatatgat ctagtgatga ataatcctcg ggcagaagat cagcaaaaaa tcaaagaact
    15901 ttcaccaaca ctttctgctt cttatactca aaaagaagat gcgggaacga tttactataa
    15961 tgcaagtgaa tttgcggctc aaccattgat catgataaaa catgaagatg gaacaccgcc
    16021 gaagatcacg aaagaaaaag ttcctgctaa aaaaatgaat gaactgactg tacaagagga
    16081 acttcgaagc tatgaattgc cggaacagca agaaaaagaa atcaaggtag tcagtcaatc
    16141 agaatttgat caactgaact tgccacaatc cactttacag ctagttcagg taaaagactt
    16201 ccaagcaaac ctgaaaccaa tcgaaacact tgttgctcaa aataaaatga acaatccatc
    16261 tttgcaaaaa gtagagtaca gcaaccagaa atatgaaatg tatgaagtgt ataatggtct
    16321 attctctggt tttgaattta tgggattctt cctaggttta gctttcttga ccatgcttgc
    16381 tagctgtttg atgttcaaga tcttgtcagg aagcaagagt gacattgctc gttatgaaat
    16441 gcttgaaaag ataggaacac gacgcgggtt gcttaaacaa tcgatccgta gagaagtagg
    16501 cgtgttattc ttagcaccag gaatcttagg tgtagtccat gtcttattcg gattacaaat
    16561 gtttggtcta ttcatgcaaa atccatacaa agatatttgg gttccgttta cgatcttctt
    16621 cgtattgtat ttcatctact atgtactgac gacatggtta tatactggaa tcgtattgaa
    16681 aaagagattg tgaaaaagct gtcctgcatc aagtaataag aacagccaag caaaaatagc
    16741 tcctcatatt tttcgtttgg ttgggcttat taccgcagat gcaagctttt gaacaccgtt
    16801 tgttcggaaa aagaggttgt gacaatattt ttgtcacaac ctttttttca cggtctttta
    16861 ttagaatata gtaaattttt tctcgttact cacggaataa gctgttaaga aaacggtttc
    16921 catgtatact ggaatcagtg caaatgaaag aaaagagggt gcaaggaaca tggaaatcaa
    16981 tgaacaaaat tttggcgaaa actatcactt gattacgatc acgaatcaaa atgggacatc
    17041 tttgtctgtc agtgatcttg gggcacgggt tgttcatttt caaacaataa tcgataatga
    17101 aaaacgagag ttgatcttag gttttgatga agcaaaagaa tatctagaaa aagaccctta
    17161 tatcggtgct tcaatcggac gtatggctgg acggattacg aatggcacat tcaaattaga
    17221 tgatgaaacg ttcgaagtaa aaacagaccc ggacactggt cattgtttac atggtggtgc
    17281 acctagcttt gaagccaagc agtggcatta tacagtcatc aatggtgaaa acgaagcatc
    17341 ggtcattttc tatcagacta gcccagatat gggaaacgga tttcctggaa ctttagatgt
    17401 agaagtacgg tatacgctga cgaacgatga tacatggcga gtgacggtcc aagggatcag
    17461 tgaccgcaaa acagtattca atcctacaaa tcatgtctat ttcaatttaa ccggtgatcc
    17521 agcacagtca atcgacgaac atatactttg gttgaatagc cgattatttg ctacgctcaa
    17581 taaagataca acgcctacag gagaagtagt ttcagtaagt ggcacagcat ttgacttcca
    17641 acgtcctaaa aaattaggtg aggtctttgc cagtgatttt cgccaatcaa tgatagtggg
    17701 cggcatcgat catccatttt tgttgaaaaa acaatctggt ttactaaatg ctgcggaatt
    17761 gacaagccca gatgaaaaag tatcgatcca agtaaaaaca gatgcttcag gaatcgtgat
    17821 ttttacagca aattttggag aagatggacc ggaaatgcgc gggaataaat tagtaaatca
    17881 tggcggtatc acatttgaga ctcaagaact gccaggagcg gagcgacttg aaagttttgg
    17941 agatgtcact ctggaaccta accaagtacg cgagtttgta acggaataca aaataaatat
    18001 aaagaaatag aataaattaa aaagggagtg tgacagtgtc acatcccctt tttaatttat
    18061 tctatttctt taaaggcaga tcaagccaga atgatttgtc taaacttaac aaggttagta
    18121 caggaacagt tgcagcttta actgtacgag tagttgtttc tggtatctgt cttgccgtaa
    18181 aattgttcca caaatcccag attgaagcga cacggaagat ggaagaagcg ttgttgacgc
    18241 gttttatcgt tgaaaattct aatcctgtga ttggcacgcc atctgttgtc catttgattg
    18301 gcacatcgaa tcgcttagac atattgagga tttcagcgtt tgcttcttca gttgttgctg
    18361 cttctttttt cggttcatca tattttgtca ggtcgtacgc ttcgatgatc gcgttcaatt
    18421 tttgtgaata ttggcgatct gtagcataac gtccttccaa atatttggtt gcttctttgt
    18481 agtcttttgt ttccgacttc caagttggtt tgtagaaatc aggattgcca gtcagtccat
    18541 ttttgatcaa gtcggcatag tcttccaaac tttccttgta agaaggatat ttacggaatt
    18601 gtgcgcggat ttggtagctt tgtccggaac tatcttgttc aagtgtattg aaggtcacgc
    18661 tgcttccttt atatgatcct ttgataccaa aaaggttatg atttggttcg cttgataata
    18721 agctgtttcc agatccactt tcaaggatag cttgagccat catgactgat gcgtagattc
    18781 cttctttttg cccgatttcc tgtgcatcat ctgcaatctt tttcaagaac tgatcagttg
    18841 tttggttttg actgaaatgg aacgagccat ctaatccatc taaaatagat gagccgcctg
    18901 tcagcgaacc tgaagcagca ggtaaacctg ctaagtttac tcggacagca aaatcaggcg
    18961 tgaaatctga aatagaagtt actcgaactt tatctcctgg ttgaggcgca tggataaatt
    19021 gtccgttccc aatatagata cctacatggt gagtaacacc ggaagaaggt ccccagaaga
    19081 cgagatcgcc aggttgggcc tgacttactg gaatctgcgt acctgcatat tgctgttcgc
    19141 ctgtccagcc accgatataa tgaccggttg ctttcatata tacgtaataa gtcaaaccag
    19201 aacagtcaaa agtatttgga ccttttgctc cccatacata tggttttcca agatgcttga
    19261 atgcttctcg aacgattgct tgttggacat tcgttgtatt tgtaggcaat acagcatcaa
    19321 ttggtgattt tacaacaaaa tcagaagaag cagaagatgg attctctata ccgctttgat
    19381 ttgctgtatt tggacgagat gaagcattgt tcgttccgcc attcgatgaa ccattgtttg
    19441 gatggtttgg ttctctctga gtatttccat tgttattcga tccagatggg tttgtttgat
    19501 tcgaagaatt cccgctgttc gaattattgt tattattgtt tgaattattt gaaccattat
    19561 tattgttgtt attacccaca tctggtttgt ttggcgtttc cggatctggt gtactaggga
    19621 cttcttcact aggatcagtt ggtttctctg ttgaagtatc cggtccagct ttaaattctg
    19681 cagtaataga taaattgttc gtacctactg taaaggtctt tgcatcataa tttacttctt
    19741 tggcaccatt aacagcgact gaagcaattt gttcaccttc agctggcgta atagtgtaag
    19801 ttaccttcgt ttccgctaat aaaccttcta ccgttccact ttccgtaata tccaaaggtt
    19861 gctgttcagg ggaatccgct gtcgaagcag tcagttggat cttaccaatc aaagaaggag
    19921 caattgtaag ggaatatgtc tcggctggct tttcattttc tgatggatca gccggatcaa
    19981 tttcagttgg ctcggtcgat cctgttgttg agttagttgt tccagttggg tcttctgtaa
    20041 aagaatccgt tgtttcacta gtagctgagc tggatgtagt cgtatctgtt gtgactgttt
    20101 gatcagctat ttgattatct acggcaaata cggtattggt cgacaaaaca gacggagcaa
    20161 tcagccaagt agcagctagc aaacgtatcg tttttttatt tttcataata aactagtcct
    20221 tttcctttca ttgattttct gtcttattca gcattcatta gacatttttt ggaacagata
    20281 atcgtgtcga aaagcttttt cttttcataa atctgtctgt gtattctaaa taaaacaatt
    20341 tgtaaataag caacgagcat tcattcgttt ttctttgacg ttcattttat tgcatagacc
    20401 acctttgttt ggggataaac aagtggggat aaaaatggtt atgcatatgt aaattatacc
    20461 aaagtttgga aagtatcaca aaaatttata aaaataatga tcctaaaaag aaaaagactg
    20521 aaatatgtga taaacatctc atacttcagt ctttttcatt aactattcat aagacattag
    20581 gtcttcatca aacccgctaa cgagagtagt gatcattgct acgactagac taacagccag
    20641 tcctgctaag taccagaaaa aagtaccgga aatgaatgca ggcaatgtgg tcagactacc
    20701 aaatacaaaa gcgatcgctt tcacttgggc aaatcccatt acagctccac cagcagctgc
    20761 accaatgcaa gcagcaacaa atggtttttt caatgggatc gcaattccgt aaacagttgg
    20821 ttcggtcact cccgcaagta aaatcgaagc aaaagcagaa ccggagatcg aacgggtttt
    20881 tgaacgttta cttcgcagaa atacacctaa agctgcacca gccattccaa aattggctgc
    20941 tcctgtcaac ggaacgattc ggtcgaaccc ataagcagag atattactga tcatgacagg
    21001 gtttacagcc cattggatac caaatattac caaaatattc caaccaccag cgatgattgc
    21061 gccactgatc ggtccgttaa tatccatcat ccaagtaatt gcggcagcta aaccttcgcc
    21121 aaggtaaaca cccgcaggcc cgaataccat taacgtcaaa ggaaccgtga taactaatga
    21181 aagtaaaggt acaaatacta attgcatatt ttcgcgaata tattttttga ggaatttttc
    21241 aacaaaagaa taaaagaaaa tacctaaaat cgccggcatg acagtagacg tatactgcat
    21301 cagtactaca ggaatcccca gaaaatcagt agctgctttg ccactgtctg aaagtaaaga
    21361 ggtcagattc ggttcaatca gtgctgcagc aatgacagcc gcaatatatc cattcacttt
    21421 aaacgttttg gcagcagaaa aagcaagaaa gattggcaga aaataaaaga cggcattcga
    21481 cgcagcagac aaaatatgat aagttccgct tgtttcacta agccagccaa tcgtagtcgc
    21541 taaaacaacc agacctttta atataccaga accggcaaac agcggaatga tcggcgtaaa
    21601 gatttccgat atcaaaccaa atagtttttc gatcggattt agcttttctt tcggagctga
    21661 atgatcgatt tgacgcgtca attccttgta agcaggagcc aatccctgat ccatgattat
    21721 ttctgcatat ctatcgagta aaatgaaata cttaatggaa gggatttgct gttttaattt
    21781 ttctgcattg gctactggct gttttttcaa tttgatacga agccgagtcg cacatggaag
    21841 gacagaatca atattgtctt ttcctcccaa ctgttcaatc agcatatgaa tctgctcgga
    21901 cagtaccatc tgttcaggat tcattgtcgt ttccattaga agaccactcc actcactaat
    21961 ataatattgg cgtaaggtgt catttcgcct gtacgaataa atgcatgaca gttattcaac
    22021 tcttttttca tttcttcatg tgggataaag ctaatcggtg tatctggtaa caataatttg
    22081 attttttcca atatttccgg gttcattgtc tttatttctt ccgccaattc aatcgattgt
    22141 acctcaagtt cttctaaaac gttttctaaa acagataaaa aacttggaag accgtttgag
    22201 acagccaaat ctattttttc agtaccaaag gggacaggca tgcctgcatc cccgatactt
    22261 aatttatcaa agtgccccat ttgtgcgatt actcgtgaaa tatctgaatt gattactttt
    22321 ccttttttca tgtttgtttc ttcctctcat tgttaatttt agactgctga ttgttctaaa
    22381 gcttcgcggt aagggataga aggttgcgca ccataagact gcacagcaac agaagaggct
    22441 agatttccat aaaggatcgc ttctttcaag ttactaaggt ctggctgaag tgtgctgctt
    22501 aatgcgccaa taaatgtatc acctgcggct gttgtgtcaa cggcatccac tttaaaagca
    22561 gggacgatac cgtgttcttt ttctgtatga tagaaagcgc cggcactgcc tagtgtaatg
    22621 attactgttc caatacctaa ttcatggagt ttttcggctg ctgcgaccaa actgttatga
    22681 tctgtcactc tgattcctgt aatgatttct gtttctgttt catttggtat aatgatatct
    22741 gtcttttcaa gtagttcagt aggaatcgtt tcacgagctg gtgcaggatt caggatcgtt
    22801 gtttttcctg cgtcttttgc aatagaaaaa gctaggatcg tcgcatctaa gctactttca
    22861 aactgagcaa taataaagtc actgtttgta atcagttgct tgttttttag gacttcttgt
    22921 tcatggaaag cattatttgc ccctgcatgg atcaagatac tattttctcc tgctgcgtct
    22981 accataatca ttgcttgtcc ggttgtggcg ccttggattt cagctatccc agatgtatcg
    23041 atgttttctt ttgtcagtag atctaataac tgttgtcctt cgctgtcagc cccaacgcct
    23101 ccaataaaag atgtccgtgc gcctgaacgt ttggctgcca ctgcttggtt ggcaccttta
    23161 cctccgccag cgtgaaaaat ctcatgcgca tgcatcgttt cgcctggctt gggcatgttg
    23221 gttaaacgaa gggtagtgtc catattaata cttccaatta ctgtaaccgt gttcatttta
    23281 caaacctcca actatctcat ttattttgat gggaaaatta ttttttaaaa cgttttagta
    23341 aaacgtttta atagttcgat aaaaatatag cacaccttga aagctatttc aagggtgcag
    23401 ttgaaaatct tttttctaat cgtacaggta atcttttttc ttcccattct ttttctggga
    23461 attggatccg atctaacaat aacttggcag atgtttgtcc caattcgaag attggttgtg
    23521 cgatcgttgt cagtttcggc ttgatatact cgcacatatc tatattatca tagccaataa
    23581 tgctgtagtc ttcagggatg ctttttccag cttcctctaa tccgcgatat aatccaaaag
    23641 ctaattcatc atttaaagca aacacaccag ttgcagcaga atcaagaagt tctgctgtga
    23701 tctggtagcc accttgtttt gagaactgtg taggtaagag gatcagttgg tcgtgaggga
    23761 tttggtaaac atcaagagca gatttaaacc cctcaatccg agcgtggaca ttttccggtg
    23821 gattttcagg atagacaagg gcgatcgttt gatgtcctag actaagcaag tgcatgcccg
    23881 ctaggtaacc accgcgaaaa tcgtctgttc gtacggcatc actaaatcct tcagattttt
    23941 tctgatccaa aacaatgaag ggccggcctt gttttttcaa attttcgttg attgcatctg
    24001 tagagacagc agaagtcgcg ataatgaatc catccacacc tcgacgtgtg agctcggcta
    24061 gatattcgat ttctttttga tgatcagaat cagcattaca taatatcgtg acgaaattct
    24121 gcttatacaa gatatcctca attcctcgca tcagtgtact gaagaatgga ttcgtgatat
    24181 caggaacgag tacaccaatt gttttcgttt ccttggtaat catttgtcta gcaaaatagt
    24241 caggctgata gcctaattct tctttggcag ctaaaacttt ctgaacagtt ttcggactga
    24301 atttctccgt atttccattt aatatcaagg agacagttgt gattgaaacg ccggaaagtt
    24361 tagcgacttc tttgattgtg atcttttttt tcaccatgtt catactccta tctaaatcct
    24421 gtggttattt tcccatgaaa tcaaaaagaa gtaaatgaaa atcacgtgtg ccagtactta
    24481 tttgctttgt tggatagctt cgattatttg tttcgttgag cgtactcgtg cagttttcgg
    24541 aaatatcttt tttatgaaca attgatggga ttctttgtca cgatcactgc aagcatcttc
    24601 agcaagcacg atatggtacc cgtgttgaaa ggcatctaat gcagtagcat atacaccgtt
    24661 tgttgtcgct acaccgctta aaatgatagt ttcgattcca cgccggcgaa gttgaagatc
    24721 aagagaagta ccaaaaaaag cacttggatt gtattttttg atagtcaaca tattatctgt
    24781 atctttcaaa gaatccttca aaagtaaatc agtgaattct ggaggtacag aagcagtcat
    24841 tccacccata tcagtttgtt gactcaaata agggaaagtg gagatgtcta cgttcactaa
    24901 aactttcaag gcagcggtac gtttcaatgt tcttactaac aaatcattgt ttgtcagaat
    24961 agatgctgca gtgtgtggag agagtgtacc gttatttacg atctcttttt gcatgtcgat
    25021 cgcgacaaaa gcagtttttg ataaatcaaa aatcatattt tcctttaaat cattcatgat
    25081 gttccttctt tcaaataaca atgtttttta tgtaagtaaa tcataaatcg tttatttgtc
    25141 aactaaatta aaggattcct ttctttagtc tagagaaatt cagtggtaaa ataaataagt
    25201 gtgagaaaac gcattcgatg catagatata gcgagaaata tcgtgaaaat ggaggaatca
    25261 gagtgaagta tgtcgaagta aaaaatgaat acaaacgata caaaatggga gaaacgacca
    25321 ttacagcaaa tgacgatatt tctttttcaa ttgaaaaagg cgaattagtg attatacttg
    25381 gtccaagtgg agcaggaaag tcaacgattc ttaatatttt aggtgggatg gatacacctg
    25441 atgaaggtca gatcattatt gatgacacag atattgcgca attttcagat aaacagctta
    25501 ctgcttaccg acgaacagat gtagggttcg ttttccaatt ctacaatctc gttcctaatc
    25561 taacagcaaa agaaaatgtg gaattggcaa cggaagtttc tcctgatgcg cttgatcctg
    25621 tagaggtatt gaggcaagtg ggcctagctc atcgattgaa taactttccg tcccaattat
    25681 caggaggcga acagcagagg gtttcaattg ctcgtgcact agctaaaaat cccaaacttc
    25741 ttttatgtga tgaaccgaca ggcgcgctgg attttgaaac gggcaagcaa gtattgaaat
    25801 tattgcaaaa cgctagcaga caacacggca ataccgtttt gatcattaca cataattctg
    25861 ctttagcgcc catcgctgat agagtcattc acattaatga tgctaaagta aggtctgttg
    25921 aattaaatga tcatccttca tccattgatg agattgtatg gtagggggat tttatgaaaa
    25981 caagaacata tcttaaagca ggttttcgcg aaattcggca atctaaagga cgattcattg
    26041 caatcatcct tattgtcatg ctaggaacac ttttatttgt aggtgtcaaa actgctggac
    26101 ctgtcatgca aaaaacgatg gatcactatg ttggtacagc aggactttct gatttgcaaa
    26161 tcgtttctac tggtggcttg acacaaaaag atattgctca agcagaaaag atcccagatg
    26221 caaaagtaga aacaggaaaa cagatctatt attccaatac tggcaaaaac gaagtgattc
    26281 aaatattttc ttataacaaa aataacaagc agaacaagct acaattaact gatggaaaac
    26341 tcccggaaaa accaaaccag cttgtattgg atgaaaaagc ggaagatgaa gggtataaaa
    26401 tcggtgatat ttatcagatt gattcagatg aattgaaaga aaaagagtat accatcacgg
    26461 gctttgtccg ttctccattg ttcatcaaca atttggagag aggatacgcg aacgtcggaa
    26521 atggtagcgt cgattatttt gtctatttac ctgaatccgt ttttaaaaca gatatttatt
    26581 ctgtcttata tcttgatttt acaaacgtaa agaatttgaa tacgtacagc aaagcttaca
    26641 aagataaaat ggagcaaaat caagaaaagg cagaaaagta tttacaagat cgacccgaag
    26701 aacgcttaga agagctgaaa aaatcggcca acgaagaatt agaaccggca cagcaaaaaa
    26761 tagcggatgg caaagcacaa acaacgcaag caagaacacg gttagaagat gctaaaaaac
    26821 agttagcgca acagtcagca actatccagc agcttcctga agcgcaacga acaatcgctg
    26881 ttcaaacatt acctcaacaa gaagctcaac ttgctgaaca agaaaggcaa ttagttgaga
    26941 aagaacgtga actggcctct gcagaggaag aattgaatcg aaatgcagaa aaaattgaaa
    27001 atttaactaa accatcatac ctatacaatg aacgttcaga aaatgtcggt tttcaagaat
    27061 ttggtgatct gtctgatcgt atcgctgcga ttgccaatgt ttttcctgtt ttctttttct
    27121 ttattgccgc attgatcacc tttacgacaa tgacacgtat ggtagaagaa aaccgcaggg
    27181 aaatcgggac attaaaggcg ttaggttata cgaaagttga aatcgcaaaa aaatatgcaa
    27241 tctatgcaag tctagcctca gggataggta ttgttttagg aacgatatta ggaacgaatc
    27301 ttttgccaag gatcattttt gagctgtcta acgagcggta tgatattggg agtgcgctta
    27361 ttttttatga ctggccgccg attttacaag cagctgccgc attcttcatt gccgcatttg
    27421 gcgctgcaat gatcgtttta ttcaaagatt taagagaacg gcctgctgca ttgcttcaac
    27481 cgaaagcacc gaagccaggg aaacgcattt ttcttgaata catcactccg ctgtggtcac
    27541 gccttagctt taatcaaaaa atcagttacc gaaatctctt ccgatataag tcaagaatgc
    27601 ttatggcgat tatcggaatt gctggatgtg ccgggctgat ggttgcaggc gtgggattga
    27661 aagattctct aagttctgtt tcagccaaac aatttggacc gatcattgac tatcaagcaa
    27721 tcgttacatt ggattcatcc acggaagata gtcgtcaaaa agcagcggat atgctgcaga
    27781 agcaagaaaa agtaaatgag atcctagcta ttcatactca aaccgtagaa ttgagaaaaa
    27841 aaggacatgc ttcgcaaagt ctgacagtga tcgtaccaag taaaacagct gatttggatc
    27901 gttttatcca tttaactgat cttgaagaga acccacgctc cttgcctaaa acaggaatcg
    27961 ctattacgca aaaagtagca gaactatttg acttatctac aggagatcat atttcgcttt
    28021 atgatgaaga tcaaaaagaa tggaaagtcg aagtaaaaga aatcgtgaaa aactatctag
    28081 gtagctttat ttatatgtcc cctgaatatt atgcttcagt tactgatcaa tcgatgatgg
    28141 ataatgcatt tttgctgcgt acagataaga tgacaacaaa agaagaagaa atgctttccg
    28201 agaaactgct tgccacagac gctgtgacaa atacgtcttt catgtccaaa caaatcgaaa
    28261 cgcaagaaga atcaatggcg aacttagact cggttgtctt aatctttgtc gtattatcag
    28321 gactgctggc ttttatcgtc ttgtataatc taactaatat caatatttcc gagcgtgtac
    28381 gcgaattatc tacaataaaa gtccttggat tttttgataa agaagtcacg atgtacattg
    28441 ttcgagaaaa tatcatcttt actcttttag gaatcatcgg aggatttggt gtaggatata
    28501 tattgactga cttcatcttg caacaagcgt ctatggagaa tgtcatcttt ccattagtga
    28561 tcacatggtg ggcttatgtc ttatcagcag gattaactat cgtatttacg gttattgtga
    28621 tgattgttac ccatttcaaa ttaaagcata tcgatatgat tgatgcgttg aaatcgaatg
    28681 aataaaaaca gaggttgtga aatcagcgtt ttgagctaat aacgagggtc agctgctaat
    28741 tgaacaccat gaatcagagg ttgtaaaaaa gccgtcctgc atcgagcaaa aagaatactc
    28801 aaataaaatt agctcctcat gttttacatt tgagaagact gattgccgta gataaaagct
    28861 tttgaacacc atttatagaa gaaacaagaa tattttgcta ttttaaacaa gaggatagac
    28921 aaatacatgt ctgatctctt gttttttatt gggcaacaga cctcttagaa tgtataaaga
    28981 taaattttac aaacgctttc tgttcagtta taataataaa tgataacttg tatatataag
    29041 ctgtggtagg taataaatca gttgaatgga ggtaaatatc catgacattt aaagacaaag
    29101 tcatttacca aatctatccc aaatcttaca aagacagtaa tggtgatgga attggagatt
    29161 taaaaggaat caaagaaaaa ctcccgtatc tgaatgatct aggcatcgac atgatttggc
    29221 taaaccctat ttatccaagc cctcaaaaag acaacggtta tgatatctct gattacacag
    29281 ccatcgatcc tatctttggt acgatggagg aattcgaaca gctaatagcc gaagcaaaga
    29341 aataccggat agaaatcatg ctcgatatgg tgctgaatca cgtttcgaca gaacatgaat
    29401 ggttcaaaaa agcactagca ggggaccagc attatcagga cttcttcatt ttaagagaag
    29461 agccaacgga ttgggtatca aaatttggtg gaagtgcttg gtcgccattt ggagatacgg
    29521 ggaaatatta tttgcactta tatgatcgga cacaagctga cttgaactgg cggaaccaag
    29581 cagtaagaga ggaactattc aaagttgttc gtttttggat ggaaaaaggg gtaaaaggat
    29641 tccgtttcga tgttatcaat gttatcggta aagacgaaca attgaaaaat aataaagaaa
    29701 atgatggcaa acctgaatat acggacaaac caatcaccca tacgtatcta aaagaattga
    29761 accaagcaac gtttgggacg gattccacta tcatcacagt cggtgaaatg agctctacga
    29821 cgatcgaaaa ttgtatcttg tatacagaac ctagtagaga agaattatca atggtcttta
    29881 atttccatca tttgaaagtc gattatgaaa atgggaataa atggtctact cctccatttg
    29941 attttgcaga attaaagcga ctatttcata catggggaga aaatatgagt gagggtaacg
    30001 gctggaatgc tctatttttt aataatcatg atcagccacg tgcgctcaat cgatttgtag
    30061 ttatcgatca gtatcgaaaa caaggagcag agatgctagc aaccatgatc catttgaacc
    30121 gtgggacgcc ctatatttat atgggagaag aaatcggcat gatcgaccca gactattcat
    30181 cgatttccga ctatattgac gtagaaagtt tgaatgctta taagttactc cttgaacaag
    30241 gattcacaga aaaagaagcg tttcgtcgga tcaaagcaaa atctcgcgat aactctcgaa
    30301 cacccatgca atggtcagac acagaacaag caggatttac tacagggaaa ccttggttaa
    30361 aagttggagg caagctagaa gagattaatg tagaaaaaga actatcttct gaagattcta
    30421 ttttgtccta ttacaagaaa ttgattcggc tgagaaaaac atatccaatc gttgcacaag
    30481 gagattatca tgcttatgaa gcaaatcatc cggaagttta tggctttctt cgccaatttg
    30541 aaggacagca gctgcttgta ttaacaaatt tctatgataa agatacaagg atcgccatcc
    30601 cagaaggatt cacagaagcc aatattctta tcgataatta ttccgatact gttcttcatc
    30661 aaagtctgga gctaaaacca taccaggcaa ttgctttgct gaaaaattaa aacaaaaaca
    30721 caaaacgtcg ttcctacgag ctattctcgt aagatcgacg ttttgtgttt tatttaaaat
    30781 aatcttcttc agaaaatgaa gagttttcat actctccttt atcgccataa aatttattgt
    30841 agcgttgttt gaatttacgg aaaataaaag taacgagtaa tttgactaac gtatagatag
    30901 gaataccaaa aataacgccc acaattccca ttagatcacc catgacaagt aaaacgatta
    30961 agatcgtaat cggatggatc tgtaaacgat cacccattac tcgaggaatc accaagtcac
    31021 cgtggagaag ttgtacggca aaccagacaa tgacaaattt gacagccata aaagtagagt
    31081 cttggaatgc aatgatcaaa cctggaaaga aggcgataaa cggcccaata tatggaatga
    31141 tacataacaa cccagcagaa atagctaaaa cactagcaaa ttctaatccg atcagtaaga
    31201 aacaaaccca gtagactgct ccaaggataa atgaagcaat gatttgcccc tttagaaatg
    31261 aacccaattg ttgattagca atctttgtca aatttttaaa atctgtacga aatgctggtg
    31321 ggacaatcgc taaaactgac tgataaaatt tttgaggatc tctcaaaagg aaaaaagcga
    31381 caattggacc agtaaataaa gtaataaaaa tagctgtaac agttgaaaaa atgttgctga
    31441 tccgttgtgc gccactttcc caataatttc caataaatcc agctatatca gacgtcacgt
    31501 cgtcaataga agcaaaaaat tgattaaatg aatcggcaaa aggtgttttc tccaaaaatt
    31561 gtcggagact ctcttgaaaa tcttgaaaca aatctggcaa actactgatc aatgtctttg
    31621 cctgttcttc aagaaaagga ataagccata tgcttccaag ccccaataaa aataaaataa
    31681 tcacataaac gataatgact gacaagttcc tcgaaaggtg cttgctcaaa cgtttgatga
    31741 ctggttccaa aagataatac aagatcagtg caaacaataa tgggggcaag atagtagcga
    31801 taatgattcc gacaggcttg aatataaacg agatctgatg gaaaaaataa atgatcagcg
    31861 caagtaagat aactattcct aaaacatagt aagaggtttt tcctcctaaa aagcggatca
    31921 aacgattcgt tgtatctgtt tcttttttca ctctttttcc tcctttatct ataacttcat
    31981 tatagacaaa agcgggtata aaaggaaaat gatagaggtt gattgcaaaa atatcgtcaa
    32041 tcattcatat aaactttctc atcaattaaa ataagaaaac gcttgacagt tcttttaacc
    32101 atatgataaa ttaaatcctg ttgctaaaac gtaactatta cggtttgcga aaagcgaatc
    32161 ggaacattca aatgaaagaa gatgagaaga ggagtgtcat aaatgaaacg attgataggt
    32221 atattggcca tgctggtttt agcaggtatg ttaactgctt gcggggcaag tgggaaagcc
    32281 gaggacagta aagaaaaatt gagcgtgatg acgacctttt atcccatgta tgattttaca
    32341 aaagcaatcg tcggggatga aggagaagta gagttattga ttccagcagg gactgattcc
    32401 cacgattatg aaccttctgc taaagacatg gcaaagatcc aagatacaga tatctttgtt
    32461 tataatgatg aaaacatgga aacatgggtc cccgcaatcc aaaaaacatt acaagaaggc
    32521 aatgtccata cgataaaagc gacagaaggt atgcttttac ttccgggaag tgaagaagga
    32581 catgatcatg accatgaaca cggggaagaa ggccatacac atgagctaga cccacatgta
    32641 tggctagctc caagtttagc aatcaagcag gtagcaaaca tacgtgatca gctgattgaa
    32701 gcttacccag aaaaacagga agtatggaca aaaaatgcag cagcctatac cgagaaactt
    32761 caagcactcc atcagcttta tcaagaaacc tttaagcaag caaaacaaag atcttttgtc
    32821 acgcaacatg ctgcattcaa ctatctagcg ttagaatatg gattgaatca agtatcgatt
    32881 gctggtcttt cttctagtga agaaccgtct gcggcgagaa ttgctgaact gaaacacttt
    32941 gttaaagaac atggaatcaa ctatatttat tttgaagaaa acgccaaaga tagtattgcc
    33001 agaacgttag caaacgaagc aggtgtatct ttagaagtat tgaatccttt ggaaggattg
    33061 acgaatgaac aaatagaaaa tggagaaaac tatctttcta tcatggaagc aaacctcgaa
    33121 gcgttgaaaa aaacgacaga gacagaaaac ccgctagaag atactttgac ccccaaaaaa
    33181 gaaaagagcg tctacaacgg ttattttgaa gacgaggatg tacaagatcg ctctctttca
    33241 gattgggcag gaacttggca aacagtagat acgtatgtag aagatgggac ttttgatcct
    33301 gtttttgaat ataaagaaaa attgaatcag gataagacag cacaagaata taaagaatat
    33361 tatacgaagg gctatcaaac agatattgct tcaatcaaga tcagtgagaa agagatggca
    33421 tttacgttta aggatggaac aacgaaacgt tcaaattatc gttatgtagg gaaagaaatc
    33481 ttgacctacg aggcaggaaa ccgtggcgta aggtatctat tcgaagcaga agataaggaa
    33541 agtggcgctt ttagatatat tcaattcagt gatcacacga ttgcaccggc tgactccgaa
    33601 cattttcatt tatatgcagg aaatgaaagt caagaagtat tgctgaaaga aatggagaat
    33661 tggccaacat tttatccaga tacaatggat ggacgtgaaa ttgcacagga aatgcttttg
    33721 cactgatagg caaaaa
//
LOCUS       DACEEZ010000020        33612 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20313_2017
            SAMN12639966-rid9879223.denovo.020, whole genome shotgun sequence.
ACCESSION   DACEEZ010000020 DACEEZ010000000
VERSION     DACEEZ010000020.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639966
            Sequence Read Archive: SRR10023158
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 33612)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 33612)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741683.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741683.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:44:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,016
            CDSs (total)                      :: 2,962
            Genes (coding)                    :: 2,889
            CDSs (with protein)               :: 2,889
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 36 of 73
            Pseudo Genes (incomplete)         :: 29 of 73
            Pseudo Genes (internal stop)      :: 18 of 73
            Pseudo Genes (multiple problems)  :: 8 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..33612
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639966-rid9879223.denovo.020"
                     /isolate="E20313_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            <1..160
                     /locus_tag="IWO89_001049"
     CDS             <1..160
                     /locus_tag="IWO89_001049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ0455820.1"
                     /translation="KAVCLISSELPEILGMCDRIYTMNEGRITGEVSREDADQEVLMK
                     LMTKEEVG"
     gene            162..1367
                     /locus_tag="IWO89_001050"
     CDS             162..1367
                     /locus_tag="IWO89_001050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAQ0455821.1"
                     /translation="MEQAKNLTDKKEKKLNLKSRAADLFGRYSMVIILAGLLIAFQLM
                     TDGIFWRPLNITNLVLQNSHILVLAAGMLLVVLLGHVDLSVGSVMAFVGAIAGMMMVN
                     RQMNPWMTVIACLAVGGLIGAWQGFWVAYAGIPAFIVTLAGLLMFRGLTQVVLGGQSL
                     APFPAVFQKMSTGYLPDLSEGSSLHFLTLILGMIITIVLVVGQWRTRNRRKNHLFEVE
                     SPTAFLVKAVFTAVVVLGITYIFASYQGYPIILIILGLIVAAYGFLTNKTVAGRQIYA
                     TGGNRKAAELSGIKTKRITFWVFVNMGVMAALAGLVLAARLNAATPQAGTSMELDAMA
                     AVYFGGASTSGGIGTIMGAIVGGLVMGVLNNGMSILGVGVDWQQAIKGLILLLAVVLD
                     LYNKKKRTT"
     gene            1624..2601
                     /gene="guaC"
                     /locus_tag="IWO89_001051"
     CDS             1624..2601
                     /gene="guaC"
                     /locus_tag="IWO89_001051"
                     /EC_number="1.7.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GMP reductase"
                     /protein_id="HAQ0455822.1"
                     /translation="MKVFDYEDIQLIPNKCIVNSRSECDTTVTLGKHTFKMPVVPANM
                     QTIIDETIAEFLAENGYFYIMHRFDEAARIPFIKKMKKRGLITSISVGVKKEEYSFIE
                     KLAEESLNPDYITIDIAHGHANSVIDMIQHIKKYLPETFVIAGNVGTPEAVRELENAG
                     ADATKVGIGPGKVCITKIKTGFGTGGWQLAALRWCAKAARKPIIADGGIRTHGDIAKS
                     VRFGATMVMIGSLFAGHEESPGETKVENGIVYKEYFGSASEFQKGEKRNVEGKKIWIQ
                     HKGSLKDTLVEMQQDLQSSISYAGGRDLEAIRKVDYVIVKNSIFNGDTI"
     gene            2865..4289
                     /locus_tag="IWO89_001052"
     CDS             2865..4289
                     /locus_tag="IWO89_001052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HAQ0455823.1"
                     /translation="MWEKVEKKQGPIYRQIMEQIMREIENGRINPGSQLESERKLGQL
                     FGVNRSTVVHALEELRELGVLTSKRGSGWFVNQTEWGKFAVPRIDWRQMISPRYEQTD
                     WYEQKIREAKQIQKTSFLDLYASEMPDELLPNFEFPSYSLEEILAEEKEMDLLGYTPL
                     RNKIKRYLESKFELAFSPNQLLVTGGGQQAIFLILQTILSAGEGIAVESPSFFYRLPL
                     FKASGTRLFGIPMDEEGIDLEKLKESIHKNKLKAVLINPNFQNPTGKVMSQKRREKLV
                     ELCRQYRLPIIEDDVFTDLSFLNQEEVLPIRTIDPDNVLYVGSLSRVLGKTTKIGWVI
                     GPETFISQLAQAQEMMEFSMNILTQITVANVFNEAIDLKMQDVRKKLKEKSRLISAWG
                     DSQSFFKLHSILGGYYAWITWDGKKLDPELAEKVVDMGLGIAPSLLFGQDINGIRINY
                     SRLDENQIPLFYSKMKILEKWLTE"
     gene            4750..5859
                     /gene="pdhA"
                     /locus_tag="IWO89_001053"
     CDS             4750..5859
                     /gene="pdhA"
                     /locus_tag="IWO89_001053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase (acetyl-transferring) E1
                     component subunit alpha"
                     /protein_id="HAQ0455824.1"
                     /translation="MANKKAPIDFQALLDEVNADFPVYQALDQDGNVVNKDLVPDLSD
                     DELVELMTHMVWSRVLDQRSTALNRQGRLGFFAPTAGQEASQLASAYAFDKEDVLLPG
                     YRDVPQLVKHGLPLSQAFLWSRGHAAGNSYPEELKALPPQIIIGAQYVQAAGVALGLK
                     KRNKKNVVFTYTGDGGSSQGDFYEAINFAGAYHANAVFYIQNNGYAISTPREKQTAAK
                     TLAQKAVAAGIPGIQVDGMDPLAVYTVSKMAREWAISGNGPVLIETLTYRYGPHTLSG
                     DDPTRYRTKDTDDDWQKKDPLIRFRKYLTEKGLWSEEKEEQVIEATKEEIKAAIAEAD
                     KVPKQKVSDFLKNMFEVSPQSIKEQIAIYEAKESK"
     gene            5862..6839
                     /locus_tag="IWO89_001054"
     CDS             5862..6839
                     /locus_tag="IWO89_001054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoacid dehydrogenase subunit beta"
                     /protein_id="HAQ0455825.1"
                     /translation="MAQKTMIQAITDALALELENDENVVVFGEDVGKNGGVFRATEGL
                     QEKFGEDRVFDTPLAESGIAGLSFGLALEGFRPVPEIQFFGFIFEAMDEVVAQMARTR
                     YRMSGTRKLPITIRSPFGGGVHTPELHSDNLEGLIAQSPGIRVVIPSNPYDAKGLLIS
                     SIRSNDPVVFLEHMKLYRSFREEVPDEAYEVPLDKAAVTREGTDVSIITYGAMVREAI
                     KAADNLAKENISVEIIDLRTVAPLDVETIIQSVEKTGRVVVVQEAQRQAGVAAQVVSE
                     ISERAILSLEAPIGRVSAPDTVFPFGQAENVWLPNAKDIEDKVKEIAEF"
     gene            6864..8159
                     /locus_tag="IWO89_001055"
     CDS             6864..8159
                     /locus_tag="IWO89_001055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxo acid dehydrogenase subunit E2"
                     /protein_id="HAQ0455826.1"
                     /translation="MAYQFKLPDIGEGIAEGEIVKWFVKPGDTINEDDTLLEVQNDKS
                     VEEIPSPVTGTVKNVIVPEGTVANVGDVLVEIDAPGHNSAPSTSAPSAEAPKEKVETS
                     GSASVVEAADPNKRVLAMPSVRQFAREKDVDISQVTATGKGGRVTKEDIENFLAGGPS
                     SAPAKSEAPEAAAPKEAAPAAESKPAAPAKPFKSNLGDLEERVAMTPTRKAIAKAMVN
                     SKHTAPHVTLHDEVEVSKLWDNRKRFKEVAAANGTKLTFLPYVVKALTATVKKYPVLN
                     ASIDDANQEIVYKHYYNIGIATDTDHGLYVPNVKDADRKGMFAIADEINEKAKLAHDG
                     KLSAEDMRNGTITISNIGSVGGGWFTPVINYPEVAILGVGTIAQQPIVNAEGEIVVGR
                     VMKLSLSFDHRIVDGATAQQAMNNIKRLLADPELLMMEG"
     gene            8167..9573
                     /gene="lpdA"
                     /locus_tag="IWO89_001056"
     CDS             8167..9573
                     /gene="lpdA"
                     /locus_tag="IWO89_001056"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HAQ0455827.1"
                     /translation="MVVGDFAVELDTVVIGAGPGGYVAAIRAAEMGQKVAIIEREYIG
                     GVCLNVGCIPSKALIAAGHHYQESLDSEMFGVTSENVKLDFAKTQEWKENKVVHTLTS
                     GVGFLLKKHKVETIEGEAFFVDDHTLRVIHPDSAQTYSFNNAIIATGSRPIEIPGFKF
                     GGRVLDSTGGLALKEVPKKFVIIGGGVIGAELGGAYANLGAEVTILEGSPQILPTYEK
                     DLVKLVEDDFKKKGVTVVTNAMAKEAVDNGDSVTVKYAVDGKEESVTADYVMVTVGRR
                     PNTDDMGLEQAGVEVGERGLITVDKQGRTNVPNIYAIGDIVPGAALAHKASYEAKIAA
                     EAISGKKVAVDYKAMPAVAFTDPELASVGMTIKEAKDAGIEAKAYKFPFSGNGRALSL
                     GKTEGFIRLVTTIEDNVLIGAQIGGVGASDMVSELALAIESGMNAEDIALTIHPHPSL
                     GEIVMDASELALGLPIHI"
     gene            9735..10010
                     /locus_tag="IWO89_001057"
     CDS             9735..10010
                     /locus_tag="IWO89_001057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0223 family protein"
                     /protein_id="HAQ0455828.1"
                     /translation="MQEYQYPLDLDWTTDEMVIVMKMWEDLERANEKGINNEAFLKTY
                     QQFKTVVKSIGEEKRLGREFEKASGYSLYRTVQQAKKNPNKTLRMNG"
     gene            10016..10783
                     /locus_tag="IWO89_001058"
     CDS             10016..10783
                     /locus_tag="IWO89_001058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase family protein"
                     /protein_id="HAQ0455829.1"
                     /translation="MEQMIDHIKSWLEEAGEKIKSSQQTLTISQKSNRTDLVTNVDRN
                     IQNFLIEKIHSFDPEAKILAEEEGCNQLSDLDGRVFIIDPIDGTLNFVIEGENFCIML
                     AVYESGVGKLGFIYDVMRNELFWGGKGIGVYKNDEPLPRPANKSLSEGLLGVNSYLFG
                     HNKYGIREIAESSMGTRMYGCAGLELVAILKGNHIAYISNLSPWDYAAGNVLLEEFGM
                     VTSGLFGTPLTFEKREYYLAGTSCAYAEIADLLKKEG"
     gene            10951..12780
                     /gene="typA"
                     /locus_tag="IWO89_001059"
     CDS             10951..12780
                     /gene="typA"
                     /locus_tag="IWO89_001059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translational GTPase TypA"
                     /protein_id="HAQ0455830.1"
                     /translation="MNYRNDIRNVAIIAHVDHGKTTLVDELLKQSDTLDAHTQLQERA
                     MDSNALEKERGITILAKNTAVDYKGIRVNIMDTPGHADFGGEVERIMKMVDGVVLVVD
                     AYEGTMPQTRFVLKKALEQHITPIVVVNKIDKPSARPEHVVDEVLELFIELGADDDQL
                     DFPVIYASALNGTSSLSDDPADQEPTMAPIFDTIIEKIPAPVDNSDEPLQFQVSLLDY
                     NDYVGRIGIGRVFRGTIKVGDQVALIKLDGTVKKFRVTKLFGFFGLKRLEIQEAKAGD
                     LIAVSGMEDIFVGETVTPVDHQDALPILHIDEPTLQMTFLVNNSPFAGREGKFVTARK
                     IEERLMAELQTDVSLRVEPTNSPDAWTVSGRGELHLSILIENMRREGYELQVSRPEVI
                     EKEIDGVKCEPFERVQIDTPEEYMGSVIESLSLRKGEMQDMVHTGNGQIRLTFLTPAR
                     GLIGYSTEFLSMTRGYGIMNHTFDQYLPMLPGQIGGRHQGALVSIDTGKATTYSIMSI
                     EERGTVFVEPGTEVYEGMIIGENSRDNDLTVNITKAKQMTNVRSATKDQTSVIKKPKQ
                     LTLEESLEFLNDDEYCEVTPESIRLRKQILEKNAREKASKKKK"
     gene            13041..14813
                     /gene="recQ"
                     /locus_tag="IWO89_001060"
     CDS             13041..14813
                     /gene="recQ"
                     /locus_tag="IWO89_001060"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase RecQ"
                     /protein_id="HAQ0455831.1"
                     /translation="MNDLLKQYFGYDEFRPGQKEIIQKVIDQENVLGIMPTGSGKSIC
                     YQLPALLLDGLTVVVSPLISLMKDQVDAANQLGIPATFINSSLDGYETARRFQEIDRQ
                     QYRLLYIAPERFIMPDFIQAMKRWNVRMIAIDEAHCISQWGHDFRPSYLQMANQLDQL
                     PNRPVIVALTATATVQVAADIKRLLKIPENNHIQTGFERENLRFQVIKDQKKEQYLIE
                     YLKINKNQSGIIYAATRKEVDRLYHLLKKFDFSVGRYHGGLNENERTEMQEAFLYDRL
                     QLIVATNAFGMGINKSNVRFVIHYQIPGSLEAYYQEAGRAGRDGLSSEAILLFSPQDI
                     QVQKFFIQQSQREEGQKQKEYEKLKAMTEYVYIESCLQQYILNYFGETSSPCNRCGNC
                     LDDREIVEVTTEAQMVLSCLKRMGENYGKQMLMKVLAGSKEQKLQALGFGHLSTYGLM
                     KNQSQKETMQLIEYLISNGYLLTINGEYPVLKVTERGIQVLKGQESVYRKEPKKVQQL
                     SDEETDTLFEVLRELRTDLASEAGVPPYVVFSDSTLKEMSRIRPSSRLEMLQIKGVGQ
                     SKLDKYGEAFLSRIKNADSNVLDK"
     gene            14927..15229
                     /locus_tag="IWO89_001061"
     CDS             14927..15229
                     /locus_tag="IWO89_001061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlaN family protein"
                     /protein_id="HAQ0455832.1"
                     /translation="MDSLSKKLAIQVLNDEAERIKRLIRNQNNSLCISQCKAFEEVVD
                     TQMYGFSRQVGFAVRIGVIGKNEGQLMLSELERELNQLYSEVYQEIYDKKEVGKEE"
     gene            15232..16395
                     /locus_tag="IWO89_001062"
     CDS             15232..16395
                     /locus_tag="IWO89_001062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HAQ0455833.1"
                     /translation="MPKKVKKRHLLDYSILIPYLILCVLGLIMVYSSTSYLLLENGQN
                     PSASVINQSIFWVLSLIAIALLYKMKTDVLKNQRLIMAAIAVLTILLLIVVFFGKEIN
                     GAKGWLQIAGFSIQPAEYLKIISIWYLSLTLSKRQNSVQKDFLGTVKRPLAMVIGLTA
                     LVAILPDFGNAAVIFLIILVLLLASGVNYVYTLIVGVGGFCLSTFTIWLINITNGKIL
                     PGRLQYIYNRFAIYQNPFSDELNKGHQLVNGYYAMFNGGLFGRGLGNSIQKKGFLQEA
                     QTDFIYAIVVEELGVIMGILILALLFFMIVRIVLVGIRSKDPFNSLLCIGIGAMFLIQ
                     VFVNLGGITGVIPLTGITFPFLSQGGSSLLMLSICVGFVLNISADEKRKSLGL"
     gene            16421..19849
                     /locus_tag="IWO89_001063"
     CDS             16421..19849
                     /locus_tag="IWO89_001063"
                     /EC_number="6.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate carboxylase"
                     /protein_id="HAQ0455834.1"
                     /translation="MKKVLVANRGEIAVRVFRACTELGIKTVGIYAEEDEYSVHRFKA
                     DEAYLVGQGKKPIDAYLDIEGIISIAKECGADAIHPGYGLLSENLNFAQRCEEEGIIF
                     VGPKLHHLDIFGDKIKAKAAAIEAGIASIPGTDGPIASIDDALEFAKQYGYPIMIKAA
                     LGGGGRGMRVAHDEKSAREGYERAKSEAKAAFGSDEVYVEKYIANPKHIEVQILGDTH
                     GNVIHLFERDCSVQRRHQKVVEVAPCVSMNEQQRQKICQAAVQLMKYVGYVNAGTVEF
                     LVEGDDFYFIEVNPRVQVEHTITEMITDIDIVTTQLLIAQGLDLHKEIGLPQQEGIKL
                     NGSAIQCRITTEDPLNNFLPDTGKIDTYRSPGGFGVRLDVGNAYAGYVVTPYFDSLLV
                     KVCTHGATFETAIQKMERCLKEFRIRGVKTNIPFMLNVITHPEFQSGNAKTTFIDSTA
                     TLFEFPRLRDRGNKTMKYIGEITVNGFPGIESGEKPFYEEPRMPKDLITRTDYVTAKN
                     VLDAKGADALVEWIKGQENLLLTDTTFRDAHQSLLATRVRTKDFKQIARLTGEGLPEL
                     FSSEMWGGATFDVAYRFLNEDPWQRLRKIRSLMPNTLLQMLFRGSNAVGYSNYPDNVL
                     VEFVKEAAAQGIDVFRIFDSLNWTPQMEKSIQAVRDTGKIAEAAICYTGDINDPSRAK
                     YNVQYYKDMAKELEQLGAHIIAIKDMAGLLKPQAAYRLISELKETTDLPIHLHTHDTS
                     GNGIITYSAASKAGVDIVDVAMSAMSGNTSQPSMSSLYYALVNGPRLPEITIENAQKL
                     NHYWEDVRMYYKPFENGLNAPETEVYMHEMPGGQYSNLQQQAKAVGLGHRWDEIKQMY
                     HTVNLMFGDIVKVTPSSKVVGDMALFMVQNDLTEEDIYEKGETLSFPESVVTFFQGEL
                     GQPVGGFPKKLQKIILKGRPALNERPGLLAESVDFNEVKKELAEKIGYEPKQDEVLSY
                     LMYPQVFLDYQKAYNQFGDVTLLDTPTFFQGIRLGETINVQIERGKTLIIRLDEIGEP
                     DIEGNRVLFFNLNGQRREITIKDNSIISTVQTRLKAEPTNREQIGATMSGSVLDVLVK
                     KGDNVKKGDTLMITEAMKMETAIEARFDGEVAHVYVSSGDTISSGDLLIEVTEK"
     gene            19870..20994
                     /locus_tag="IWO89_001064"
     CDS             19870..20994
                     /locus_tag="IWO89_001064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0455835.1"
                     /translation="MKRLGLFWLILLLVIVAFYMEPVLFPPKLEVKENSDQQISNQMT
                     TWKYQELKATGFSTYIGKPIDQLEKDFGQAYDRLTSGFGFETRYYENKASHLTFEANI
                     QDSRVTTVKVLQTGPADIAPFYLGMTMQDLTKITTIYTNFTFEYHDTEVGIELMEEDM
                     NYRPLIAFDNDTFAILFFDQTTNGLFSVVYLDKDSLLKLLPYQVFGEGLPHYQADKSA
                     DWESINRVKERKSTTLLNMLRREDELPIYNQTLAFQNHSQLLLHDFLTRPEEILSEER
                     MADWQKSLGSAQTTVKFSLTNDELEKLISKQKLENTSGLFIHPAIDATFSFLYWYSDP
                     YNHDRFMDPGTDSVGIAFSKENMLVLLQEENGESSDSSDR"
     gene            20984..21421
                     /locus_tag="IWO89_001065"
     CDS             20984..21421
                     /locus_tag="IWO89_001065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbF family regulator"
                     /protein_id="HAQ0455836.1"
                     /translation="MIVNEELFQLEDQCLQVAEMIKKSKTMASYLEHKQSMEESAKVC
                     QLKEAFLSKKEAFENVAAYGEYAPDYREKKRAVRKAKRQLDMDEHVAAFRYSETQLQS
                     LLDQIVQVLAEEVSNEIKVDAGSPFFETKKHFGCGGNCHAIRQ"
     gene            21405..21743
                     /locus_tag="IWO89_001066"
     CDS             21405..21743
                     /locus_tag="IWO89_001066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbG family protein"
                     /protein_id="HAQ0455837.1"
                     /translation="MQLDNSEFEITKRRGLIVWVYSLKQLKNLKKFGLLHYVSRRMKY
                     VVLYLNEETLEETEERIRKLHFVRNVERSYRPDIEMNFAEKIGNKTVLKDEGFEIEEL
                     NTEIRLAEHL"
     gene            21862..22422
                     /gene="rsmD"
                     /locus_tag="IWO89_001067"
     CDS             21862..22422
                     /gene="rsmD"
                     /locus_tag="IWO89_001067"
                     /EC_number="2.1.1.171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(966)-N(2))-methyltransferase
                     RsmD"
                     /protein_id="HAQ0455838.1"
                     /translation="MRIISGNYGGRRLKSLAGANTRPTTDKVKESIFNMIGPYFDGET
                     VLDLFAGSGGLAIEAVSRGCSHAVCVDKNYQALKIIKENIEITKEPEKFTTIKLDADK
                     AIERLAAEKQQFDYLFLDPPYAKQKIIDQIHKMEELGLFSKTAVIVCETDKTVELPET
                     VAGFHQIRKQIYGITAVTIYRKEGNQ"
     gene            22419..22910
                     /gene="coaD"
                     /locus_tag="IWO89_001068"
     CDS             22419..22910
                     /gene="coaD"
                     /locus_tag="IWO89_001068"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HAQ0455839.1"
                     /translation="MKRALFPGSFDPFTKGHLDTVERAAKLFDEVVIGVFINTSKKSL
                     FPPEERMTLITKAVSHLPNVKVMHQENQLTVETAKEIGADALIRGIRSIKDFEYEREI
                     AQMNHHLYDELETVFLLAKPEYSHVSSSILKEVLHFGGDVSSYLPPVINEALAGKRES
                     NES"
     gene            22900..23943
                     /locus_tag="IWO89_001069"
     CDS             22900..23943
                     /locus_tag="IWO89_001069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PDZ domain-containing protein"
                     /protein_id="HAQ0455840.1"
                     /translation="MKAKQWLKTVLIIIFGMLIIGFVVVPLPYYVESPGATIDLKELI
                     TVNDKEDKGSGSFSLTSVGIRQATGFLALKAKMTPFEELISEEELTGGATNEEYNQMQ
                     QYYMDSSQNAAIEQALKLADKPYKMEFKGVYVMSVEKTSNFFGKLSVGDTVTKVDGKS
                     FQSTEAFMDYVKSQKVGQTIEISYLHNGEEKTASGDLIELPTDKKAGIGITLTDHTEI
                     ESDTDVRINSGSIGGPSAGLMFTLEIYEQVTGKNLRHGKQIAGTGTINSEGEVGRIGG
                     IDKKVASADKAGVEIFFAPDDTISDDVKKEYPEIKTNYEEAKAAAKKLNSSMKIVPVK
                     TVQEALDYLEKMK"
     gene            24102..24830
                     /locus_tag="IWO89_001070"
     CDS             24102..24830
                     /locus_tag="IWO89_001070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016925838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence protein ComEA"
                     /protein_id="HAQ0455841.1"
                     /translation="MKGVMNDFYWSFFEKRTMIEKISKKILASPKLIIWMSMIVLIFC
                     IASGFLLSNLLFYRETEETAAKEILSSFSSSGEDPSAEGNDVEEDARPKVMYTDIKGS
                     VKEPGIYSFSSEERVYDVLKRAGGLLEEADSDRINFSAKIEDQQVLYIPAVGEELPEH
                     LDQLASSEGKQPTADSEPPKININTATPSELQQIPGIGSVKAQEIIRFREENGSFQKV
                     EDLQEISGIGEKTVEKLKNFVTIK"
     gene            24946..25191
                     /locus_tag="IWO89_001071"
     CDS             24946..25191
                     /locus_tag="IWO89_001071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HAQ0455842.1"
                     /translation="MIVRKETLKKPMLNVYLQNKISGIHIMNTAVSGNNSQALRERFA
                     KDVLSYTADKVFILIGTNDLAEHKQLSKETYQKICSG"
     gene            25188..25406
                     /locus_tag="IWO89_001072"
     CDS             25188..25406
                     /locus_tag="IWO89_001072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase"
                     /protein_id="HAQ0455843.1"
                     /translation="MIVQLKKDYSSDHAFFITPPAVDETKQKYRNNKLIKEYISVLTE
                     VAKRHDCEVSIFIRNFLNKWIRRIFIMG"
     gene            25542..26081
                     /locus_tag="IWO89_001073"
     CDS             25542..26081
                     /locus_tag="IWO89_001073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComE operon protein 2"
                     /protein_id="HAQ0455844.1"
                     /translation="MLEKSSKACDTNKEKVTDKSHNERIPWDQYFMAQAVLLSLRSTC
                     TRLEVGATLVREKRIIAGGYNGAVSGDVHCIDEGCYVVDGHCLRTIHAEMNALLQCAK
                     LGIPTEGSEIYVTHFPCLACTKALLQAGVKGINYLHDYRNDPYAQALIEQLGVSVHQV
                     ALDSQHFSKLQNELTGKHV"
     gene            26074..28347
                     /locus_tag="IWO89_001074"
     CDS             26074..28347
                     /locus_tag="IWO89_001074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA internalization-related competence protein
                     ComEC/Rec2"
                     /protein_id="HAQ0455845.1"
                     /translation="MSSVLERHVRNRLIFPAITSMIICLLYYTDNWGYRLLLAGIFLW
                     FSLKEGRFIAYLCILCSITVSISYRLQEYSFLEEKTTSAQITVFTDTIRANGNFLTME
                     GRLSENKQKLQLSYQVNTEAELKTWLNFRGKGIELTAEGEFISPAKKRNLYNFDQEAY
                     FRNHRISGRFQIQKIEQITMQNHWRNWLQRKRGSFISFVQSEFPQKTSVYINSLLWGY
                     KDAGFREPEEIFRESGLLHLFSLSGLHIQFYLGWLYYLFRRIGWPLHVSIIPLSLLTI
                     CYVSIAGSSISVLRASLLFLLRLLVKLLNKHYSTMDLFAVVFWLLLLFDPRFLFHAGG
                     QLSFFMSFLFILISFDGDWLKKTASHVLFTMITMPLIVWHFYEWPILGSLFTLLIAPV
                     FKFLFLPGVLFLTLFNRWLPETLLLLIEEGLILFERIIDFSSGSSLIIGRLPLVLSGM
                     YIFGLLICMDRLKEQPVKHLFYVGLFSFIFLTLPFLRFSSTIAFIDVGQGDSIFLQSP
                     FRKETILIDTGGQLHFEREKWAQGMIRPPAERNIVPYLKGQGISVIDKLILTHDDTDH
                     VGELPTLSQHFTIKKIYIGWGAGRNEPLQRHLSEAQKNGTEIIEIKHGDRISGYYDLY
                     VLTPFEKGEGRNEDSIGLWMEYNNRRFLFLGDLNQAMEKQLLLIYPNLKADVVKLGHH
                     GSRTSSNTDFLSHIEAKHGIISCGVNNRYGHPHSEVLETLEENQIKTWRTDQQGMITY
                     RWHPVFHPHGLVETMID"
     gene            28385..29431
                     /gene="holA"
                     /locus_tag="IWO89_001075"
     CDS             28385..29431
                     /gene="holA"
                     /locus_tag="IWO89_001075"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HAQ0455846.1"
                     /translation="MVSNLSIQAELQKIRKEKLASCYLILGTEKFLQDQVRTEIVKKI
                     QLEGEDDLNFLSFDMENASLDEVVAEAETLPFFGDQRLVFVENPYFLTGEKVNNGIEQ
                     NTDLLTAYLKEPLESTVLVFFAPYEKLDERKKVTKQLKKTAIIINVQQLNEKEVRQYL
                     MNTLENSSIKMERSAIDLFLRLTDLDLSKMMRELQKLVLYGQNQQQITVREVEELVPK
                     TLEHNIFDMTQYILKGKTEQALRLYEDLVMQGEETIKINAILLSQLRLFLQTKFLVKI
                     GYQQANIAETLKIHPYRVKLAMQEVKKFDEHLLVQLFDQLVEMDYQIKTGQIEKELSF
                     QLFVLRTGQLLRNR"
     gene            complement(29520..29774)
                     /locus_tag="IWO89_001076"
     CDS             complement(29520..29774)
                     /locus_tag="IWO89_001076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016175193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HAQ0455847.1"
                     /translation="MPNIESAIKRVRTSENANVKNSSQTSAMRTAIKKFEDAVASGAD
                     NVDALYKEAVKAIDMAESKGLIHKNKANRDKSRLSKKIAK"
     gene            complement(30174..30443)
                     /locus_tag="IWO89_001077"
     CDS             complement(30174..30443)
                     /locus_tag="IWO89_001077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0455848.1"
                     /translation="METFEGLLIDTYVLQEQPMMLTGINLQLEDRVINGLLKDRYLAK
                     YILRLPLNKYVLEVSGKWNRRNQLVIKKMLIKNIDEYIKIIGTPE"
     gene            complement(30913..31368)
                     /locus_tag="IWO89_001078"
     CDS             complement(30913..31368)
                     /locus_tag="IWO89_001078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF441 domain-containing protein"
                     /protein_id="HAQ0455849.1"
                     /translation="MESWLFLGIILLVAILAKNQSLMIAAAFVLIMKLIPASRSFFPW
                     LQGKGINIGVTIITAAILIPIATGEIGLRDLLEAFKSPIGWVAVFCGGLVAVLSSKGV
                     GLIASDPQITVALVFGTILGVVFLRGIAAGPVIASGITYCILQVFALGK"
     gene            complement(31506..33434)
                     /locus_tag="IWO89_001079"
     CDS             complement(31506..33434)
                     /locus_tag="IWO89_001079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HAQ0455850.1"
                     /translation="MTNQKKAFAATIGCLLFMIFGFIFDRFSLTITPLFYISAIIIGG
                     YKQTSEGLKELWNDRTLNVDLLMALAAIGACLIGNYFEGAMLTFIFCLSGALEEYTTN
                     KSQKEITALMNLQPQKAQLLKENGQMQEVDVSQLKVDNLVFVAKGAAVPIDGFLESTQ
                     ATIDESALSGESVPVEKYLGDEMYAGTLNQGNPITIRVTKTSSETVFAKIIQLVEEAQ
                     NTPTQTASFIERIENNYVKLILLAVPLMILLPHFFLGWSWDESFYRGMVLLVVASPCA
                     LVASATPASLAALSNSAKNGILIKGGIHLEKLSELKAIAFDKTGTLTKGKPVVTDSFF
                     FDEKDLAQQLLVAMEQKTTHPLAHAILQYFDCTIPEEIQYLPIEEITGFGLQTTYLGA
                     QWKVGKHAYDPETMIISKEIAEMIERLENQGKTVIYLSKDQQLIAVLGLLDIPKANTQ
                     QVISYFKSQNIHTSMITGDHSGTAKAIAEQVGIEHYYASCTPEEKTQLVKKENELYQV
                     NAMVGDGVNDAPALAAASLGVAMGQGTDAAMDIADIVLMKSDLNKFVYSHRLSLKMRR
                     IIKQNIIFSILVILLLIASNFLQFLNLPLGVVGHEGSTILVILNGLRLLRPLPDLSDS
                     SKKCKSCPLYKARLSSVK"
BASE COUNT        11151 a         5798 c         7174 g         9489 t
ORIGIN      
        1 caaagctgtc tgtcttatct cttctgaact tcctgaaata ttagggatgt gtgatcgaat
       61 ctatacgatg aacgaaggac gtattacagg agaagtctcg cgagaagatg cagatcagga
      121 agtcttgatg aaattaatga cgaaagaaga ggtgggttaa gttggaacag gcaaaaaatc
      181 ttacggataa aaaagagaaa aaactaaatt tgaaaagtag agcagctgat ctttttggtc
      241 gctacagcat ggtgatcatt ttagcgggac tacttattgc gtttcaattg atgacggatg
      301 gtattttctg gcgtccattg aatataacta accttgttct gcaaaatagc catatattag
      361 tactagctgc agggatgtta ttagttgtgc tcttaggtca tgtggatttg tcggtagggt
      421 cagtgatggc gtttgttggt gcaattgcgg gcatgatgat ggtcaatcgc caaatgaacc
      481 cgtggatgac agttatcgca tgtcttgcag taggcgggct gatcggcgca tggcaaggtt
      541 tttgggtagc ttatgcaggg ataccggcgt ttatcgtgac tttagcagga ttgctgatgt
      601 ttcgtggact gacacaagtt gttctaggag gccaatctct agcacctttt ccagctgttt
      661 tccaaaaaat gtctacgggt tatttaccag acttaagcga aggaagcagc cttcacttct
      721 tgacattgat tttaggaatg atcatcacga tcgtgttagt tgttggtcaa tggcgaacaa
      781 gaaatcgtcg gaaaaatcat ttatttgaag tggaatctcc aacagctttc ttggtaaagg
      841 cggtatttac agcagtcgtt gtactaggaa taacttatat ctttgcctct tatcaagggt
      901 atccgattat tttgatcatt cttggtttga tcgttgcagc atatgggttt ttgacaaata
      961 aaacagtagc aggtcgacag atttatgcga cgggcggaaa tcgaaaagcg gctgaactat
     1021 cagggatcaa aacaaagcga atcacgttct gggtattcgt caacatggga gtcatggcag
     1081 ctttagctgg attagtttta gctgctcgat tgaatgcggc aacacctcaa gcaggtacat
     1141 cgatggaact ggatgcaatg gcggcagtat attttggtgg tgcttcgact tccggcggca
     1201 ttggaacaat catgggtgcg attgtcggtg gcttggtcat gggtgtattg aacaatggca
     1261 tgtcgatttt aggcgtcggg gtagactggc agcaggcaat caaaggattg atcttgcttt
     1321 tagccgttgt gctagacttg tataataaga aaaaacgaac aacgtaggag aacgccggtt
     1381 attcagaaaa aagactgtga caagacattt gtcacagtct ttttttcgct aaaagcgaac
     1441 attcattgtt tcgtttgtat agactaaaaa taatatatgt ttttttacca ttcgtataaa
     1501 ttctcttttt gctcttgatt tttttgctta attgagttaa attacatagg gcgctaaaaa
     1561 aagccttgat taaattaata atgttcgtgt tttgctggat gtgaacatca ggaggataat
     1621 gtaatgaaag tatttgatta cgaagatatt caattaattc ctaataaatg tatcgtaaat
     1681 agccgttctg aatgtgatac aacggtcaca ttaggtaaac atacattcaa aatgccagta
     1741 gtgcctgcca acatgcagac gattattgat gaaacaatcg cagaattctt agcggaaaat
     1801 ggttatttct atatcatgca tcggtttgat gaagcagctc gaattccgtt tatcaaaaaa
     1861 atgaaaaagc gtggattgat tacctcgatc agtgttggcg tcaagaaaga ggaatattcc
     1921 tttatagaga agttggcaga agaatcgcta aatcctgatt atatcactat cgacatcgcg
     1981 catggacatg ctaactcagt tattgatatg attcagcata ttaaaaaata tttgcctgaa
     2041 acttttgtga ttgccggtaa tgttggtaca ccggaagccg tccgtgaatt ggaaaatgcg
     2101 ggcgcagatg caacaaaagt cgggattggt ccaggaaaag tatgtattac aaaaatcaag
     2161 acaggctttg ggactggagg ctggcaactg gcagctctta gatggtgtgc gaaagcagct
     2221 cgcaagccga tcatcgctga cggtgggatc agaacacatg gcgatattgc aaaatctgtc
     2281 cgttttggtg cgacaatggt catgattggt tcattgtttg ccggtcacga ggaatctcct
     2341 ggcgaaacaa aagtcgaaaa tggaatcgtg tataaagaat actttggttc tgcttcagaa
     2401 ttccaaaaag gcgaaaaacg aaatgttgaa ggaaagaaaa tctggatcca acacaaagga
     2461 tctttgaaag atacactagt ggaaatgcaa caagaccttc aatcatctat ttcttatgct
     2521 ggaggaagag atctggaagc cattcgaaaa gttgactatg tcatcgtgaa aaattcgatt
     2581 tttaatggag acacaatcta aaaaataaat aaaatattag gatgttgtga aaaagttgtc
     2641 ctgcattaag taataagaac agccaaacaa aaatagttct ttatatgttt tgtttggctg
     2701 gaattattat cgcagataca agcttttgaa catcattatt cagaataagg gactgtgata
     2761 agatttttgt cacagtccct tatgttttgc aaaaaaagtt tttctttttt ctcgtaaatt
     2821 cggtaaaatc aaaagaaaaa atagacaata gaaaagagaa gcttatgtgg gaaaaagtag
     2881 agaaaaaaca aggaccgatt tatcgtcaaa ttatggaaca gattatgaga gaaatcgaaa
     2941 acggaaggat caatcctgga agtcaacttg aatcagaacg aaaactaggg caactttttg
     3001 gtgtgaatcg ttccaccgtt gtccatgcat tggaagagct tagggagctt ggggtactaa
     3061 cgagcaaacg tggcagcgga tggtttgtta atcagactga atgggggaaa ttcgctgttc
     3121 caagaatcga ttggcggcaa atgatctctc ctcgttatga gcagacggac tggtatgagc
     3181 aaaaaataag agaagcaaag cagatccaaa aaacatcgtt tcttgattta tacgctagcg
     3241 agatgccaga tgagcttttg ccgaattttg agtttccttc ttattctctt gaagaaatct
     3301 tagcggaaga aaaagaaatg gatcttttag gctatactcc gttacgtaac aaaatcaaac
     3361 ggtatttgga aagtaaattt gaattggcat tttcccccaa ccagcttctt gtgactggtg
     3421 gcggtcagca agcaatcttt ttgattttac aaacgattct ttcagctgga gaggggatag
     3481 ctgtagagtc accttctttt ttttaccgat tacctctttt caaagcttca ggaacgcgtt
     3541 tatttgggat accgatggat gaagaaggga tcgacttaga aaaactgaaa gaatcgatcc
     3601 ataaaaataa attgaaagca gtgcttatca acccgaattt tcaaaaccct acaggaaaag
     3661 tgatgagtca aaaacgacga gaaaagctag tagaattgtg tcgacagtat cggcttccaa
     3721 ttattgaaga cgatgtattt acggatcttt catttctaaa ccaagaagaa gtccttccta
     3781 tacgaacgat tgatccggat aatgtgttat acgtcggttc gttatctagg gttttaggaa
     3841 aaacaacgaa aatcggttgg gtgataggac ctgagacttt tatctcgcaa ctagctcaag
     3901 cgcaagaaat gatggagttt tcgatgaata tccttacaca aatcacagtg gctaatgtat
     3961 ttaatgaagc aattgatttg aaaatgcaag atgtaagaaa aaagttgaaa gaaaaaagcc
     4021 gactgataag cgcttgggga gattcacaat cgtttttcaa gcttcattca attttaggtg
     4081 gttactatgc ctggattact tgggatggga aaaaactaga ccccgaacta gcagaaaaag
     4141 tagtagatat gggtcttgga attgcaccaa gtctactttt tgggcaagat ataaatggaa
     4201 tcagaatcaa ttacagtagg ctggatgaaa accaaatacc gttgttttat tcaaagatga
     4261 aaattctaga aaagtggtta acagaataat ttcacaaagt aaatagatta aagaatataa
     4321 cagcttggat ttaaatttca agctgtttct tttttttcgg aaaaagaata cgatataaag
     4381 aaactaagaa aaaaccatta tttgtacgtc cgttcacgat gtgtaaataa aataaatgtt
     4441 tgcaacaaaa ctgtataaca ccaacgtttt taaatagaaa cagatggttg taagtttgat
     4501 tagaaaatag aaaaaatgca gatgaaaaat aaaacgcttt ccttattgtg attttattca
     4561 ctaatgctgt taatatgcgg attggcataa tttcctttca tcgtgaaaat ttgtgctttt
     4621 agcttgtatt tctcataaat ccatgataat ttattaatga agacggttaa atagtgatac
     4681 agttttattc actgtttcaa tcgcagaatg aagagatttt cttatagatg agagaggaat
     4741 gtgtgacaaa tggcaaacaa aaaagcaccg atcgacttcc aagcactgct agatgaagtc
     4801 aatgcagatt tcccagtata tcaagcactt gatcaagatg gaaacgtagt taacaaagat
     4861 ttagtacctg atttatccga tgatgaactc gttgaattaa tgactcatat ggtatggtct
     4921 cgtgtattag accaacgttc aacagcgctg aaccgccaag gacgcctagg cttttttgca
     4981 ccaactgctg gacaagaggc aagtcagctg gctagtgctt acgcatttga taaagaagat
     5041 gttcttcttc caggttatcg ggatgtacct caactagtaa aacatggttt acctttatct
     5101 caagcttttt tatggtctcg aggacatgct gcaggtaatt cttatccgga agaattgaaa
     5161 gctctaccac cacaaattat catcggtgca caatatgtac aagcagccgg tgttgcatta
     5221 ggtttgaaga aacgaaacaa gaaaaatgtc gtcttcactt atactggtga tggcggttca
     5281 tcacaaggtg atttttacga agcaatcaat ttcgctggcg cttaccatgc aaatgccgta
     5341 ttttatatcc aaaacaacgg gtatgcgatc tctacacctc gtgaaaaaca aactgcagca
     5401 aaaacacttg cacaaaaagc tgtcgcagct gggattcctg gtattcaagt cgatggaatg
     5461 gatccgcttg cagtatatac tgtatctaaa atggcacggg aatgggcgat cagtggaaat
     5521 ggaccagtcc taatcgaaac actgacttat cgttatggac cacatacact atctggtgac
     5581 gatccaaccc gctaccgtac aaaagacaca gatgacgatt ggcagaaaaa agatccattg
     5641 atccgcttcc gtaaatattt gacggaaaaa ggactttggt ctgaagaaaa agaagaacaa
     5701 gtcatcgaag caactaaaga agaaatcaaa gcagctattg ctgaagccga caaagtacca
     5761 aaacaaaagg tttcagattt cttgaaaaac atgttcgaag tatctccaca atcaatcaaa
     5821 gaacaaattg caatttatga agcgaaggag tcgaaataat catggcacaa aaaacaatga
     5881 tccaagcaat cacagatgcc ttagcacttg aactagaaaa tgacgagaat gtcgttgttt
     5941 ttggtgaaga tgttgggaaa aacggaggag ttttccgggc aactgaagga ttgcaggaaa
     6001 agttcggaga agaccgagta ttcgatactc ctttagctga atctggaatc gcaggattga
     6061 gtttcggtct tgcgttagaa ggatttcgtc cagttcctga aatccaattc tttggtttta
     6121 ttttcgaagc aatggacgaa gtagtagctc aaatggctcg tacaagatat cgaatgagcg
     6181 gcactagaaa actacccatc acaattcgtt cgccatttgg tggcggtgtt catacaccag
     6241 aattgcattc ggataattta gaaggtttga tcgctcagtc tcctggtatt cgagtagtga
     6301 ttccttccaa cccttatgat gcaaaaggac tattgatttc ttctattaga agtaacgatc
     6361 cggttgtttt cctagaacac atgaaactat atcgctcatt ccgagaagaa gtgccggatg
     6421 aagcttacga agttcctttg gataaagcag cagttactcg tgagggaaca gatgtatcta
     6481 ttatcacata cggagcaatg gtgcgtgaag caatcaaagc agcagacaat cttgcaaaag
     6541 aaaatatatc tgttgaaatc attgatcttc gtacagttgc gccattagat gtagaaacga
     6601 tcattcaatc cgtggaaaaa actggtcgcg tagttgttgt ccaagaagca caacgacaag
     6661 cgggcgttgc cgcgcaagta gtttctgaga tctcagaacg cgctatcctt tcattagaag
     6721 ccccaatcgg acgagtttct gcacctgata cagtcttccc atttggacaa gcagaaaatg
     6781 tctggttgcc aaatgcaaaa gatatcgaag acaaagtaaa agaaatcgcg gaattttaag
     6841 aaaagaaggg acgaaacaaa gtcatggctt atcaatttaa attgccggat atcggtgaag
     6901 gaatcgcaga aggtgaaatc gtcaaatggt tcgtcaaacc aggagatacg atcaatgaag
     6961 atgatacatt actagaagtt caaaatgaca aatcagtaga agaaattccg tcacctgtca
     7021 cagggacagt taaaaacgtg attgttccag aaggcactgt agcaaatgtt ggggatgtat
     7081 tagtagaaat cgatgctcct ggacacaata gtgcaccgtc aactagtgcg ccatcagctg
     7141 aagcaccaaa agaaaaagtg gaaacatcag gttcagcaag tgtcgttgaa gctgccgatc
     7201 caaataaacg tgtattggct atgccatctg ttcgtcaatt tgcccgtgaa aaagatgtag
     7261 atatctcaca agtaactgca actggaaaag gtggcagagt aacaaaagag gacatcgaaa
     7321 acttcttggc aggtggtcca agcagtgcac cagcgaaatc agaagcgcca gaagcggcag
     7381 caccgaaaga agcagctcct gcagcagaaa gcaaaccagc tgcaccagca aaaccattca
     7441 agtccaactt aggagacttg gaagaacgtg tagcaatgac gccaacacgt aaagctatcg
     7501 ctaaagcaat ggtcaacagt aagcatacag ctccacacgt gacattgcat gatgaagtag
     7561 aagtgtctaa actatgggat aatcggaaac gatttaaaga agttgctgct gcaaatggta
     7621 cgaaacttac tttcttgcca tatgtcgtga aagctttaac agcgactgtt aagaaatacc
     7681 cagtattgaa tgcatctatc gacgatgcaa accaagaaat cgtttataaa cattactaca
     7741 atatcggtat tgcaactgat acggatcacg gactatatgt accaaatgtg aaagatgcag
     7801 accgcaaagg aatgtttgct attgcagacg aaatcaatga gaaagcaaaa ttagctcatg
     7861 acggcaaact atctgcagaa gatatgcgca atggaacaat tacgatcagc aatatcggat
     7921 ctgtaggtgg cggttggttt acaccagtca tcaactatcc agaagtagct attttaggtg
     7981 taggaacgat tgcacaacaa ccaatcgtaa atgctgaagg cgaaatcgtt gtcggtcgtg
     8041 tgatgaaact gtcattaagt ttcgatcacc gtatcgtaga tggagcaact gcgcaacaag
     8101 caatgaataa tatcaaacgt cttttagctg atccagagct attaatgatg gaaggatgat
     8161 aaacaaatgg tagttggaga ttttgctgta gaattagata cagttgtaat tggtgcggga
     8221 cctggtggct atgtagctgc catccgcgct gctgaaatgg gacaaaaagt agcaatcatt
     8281 gaaagagagt atattggcgg tgtttgtttg aatgtcggct gtattccttc aaaagcactg
     8341 atagctgcag gacaccatta tcaagagtca ctagattctg aaatgtttgg agtaactagt
     8401 gaaaacgtca aactagattt tgcaaaaaca caagaatgga aagaaaacaa agttgtccat
     8461 acgttgactt ctggtgttgg tttcctatta aaaaaacata aggttgaaac aatcgaagga
     8521 gaagcgttct tcgtagatga tcacacacta cgcgtcattc atccagattc agctcaaact
     8581 tattcgttca ataacgcaat catcgcaaca ggttcccgtc caatcgagat tccaggattt
     8641 aaattcggag gacgtgtgtt agattctact ggcggattag cattgaaaga agtacctaag
     8701 aaatttgtga tcatcggtgg cggtgtgatt ggtgccgaat tgggcggagc gtacgcaaac
     8761 ttaggagcag aagtaacgat tcttgaaggc tcaccacaaa ttttaccaac ttacgaaaaa
     8821 gatctagtaa aattagtaga agatgatttc aagaaaaaag gcgtaacagt cgtaacgaat
     8881 gcaatggcga aagaagcagt cgataacggt gacagcgtga cagtgaaata tgcagtggac
     8941 gggaaagaag aatccgttac tgctgattat gtgatggtca ctgtaggtcg tcgtccgaat
     9001 acagatgata tgggcttaga acaagcgggt gtcgaagtag gagaacgagg attgatcacg
     9061 gtggataaac aaggacgtac gaacgtacct aacatctatg ccatcggtga tatcgttcca
     9121 ggggcggcat tagcacacaa agccagttat gaagcaaaaa tcgctgctga agcaatctct
     9181 ggcaaaaaag tagctgtaga ttataaagca atgccggcag tagccttcac tgatcctgaa
     9241 ttggcttctg taggtatgac gatcaaagaa gcaaaagatg ctggtatcga agcaaaagca
     9301 tataaattcc cattttcagg gaacggtcgg gcattatcgc ttggtaaaac agaaggattc
     9361 atccgtctag ttacgacgat cgaagataac gtgcttattg gcgcgcaaat cggtggagtc
     9421 ggcgcaagtg atatggtctc tgaattagct ttagcaatcg aatcaggaat gaatgctgaa
     9481 gatatcgcct tgacgattca tcctcatcca tctcttggcg aaatcgtcat ggatgcttcg
     9541 gaattagcat taggtttacc aatccatatt tgatttgaat aatacaggcg aaacgcaact
     9601 gaaaaatcag ttgcgttttg ttttttagaa ttatcaataa tattattatg taaacttaaa
     9661 aatatgacaa cttcttggtt tattaccact tcctatgcta cactgaagag gaatatgaaa
     9721 taggagagtt gaagatgcaa gaatatcagt atccacttga tttagattgg acaacagacg
     9781 aaatggtcat tgtaatgaag atgtgggaag atctggaacg tgcgaatgaa aaagggataa
     9841 acaatgaagc ttttttgaag acttatcagc agtttaaaac tgttgtcaaa tccattggtg
     9901 aggaaaaacg attgggacgg gaatttgaaa aagcttccgg ttattcttta tatcgtacag
     9961 tgcagcaagc aaaaaaaaat ccaaacaaaa ctttgagaat gaacgggtga gataaatgga
    10021 acagatgatt gatcatatca agtcctggct tgaagaagct ggtgagaaaa tcaaatcttc
    10081 tcaacaaaca ttaacaatct cgcaaaaatc aaatcgaacg gatctagtaa caaatgtcga
    10141 tcgaaacatc cagaattttc taatcgaaaa aatccattcc tttgatccag aggcgaaaat
    10201 attggcagaa gaagaagggt gtaatcaatt atcggatctc gatggaagag tctttatcat
    10261 cgatcctatt gatggcacct tgaattttgt gattgaaggg gaaaattttt gtattatgct
    10321 cgctgtttat gaatccggcg ttggaaagct tggtttcatt tatgacgtga tgagaaatga
    10381 acttttttgg ggtggaaaag gaataggcgt gtataaaaat gatgagccat tgcctagacc
    10441 agcaaataaa tccttatcgg aaggcttact tggtgtgaat agttatcttt tcggtcataa
    10501 taaatatgga atccgagaaa ttgcggaaag cagcatgggg acacgtatgt acggatgcgc
    10561 tggtcttgaa ctagtggcaa tcttgaaagg aaaccacatt gcttatattt caaaccttag
    10621 cccttgggat tatgcagcgg gaaatgtctt attggaagag tttggtatgg taacaagcgg
    10681 tctttttggt actcctttga catttgaaaa aagggaatac tatttagcag ggacttcttg
    10741 tgcatatgca gaaatcgcag accttttgaa aaaagaaggc tgaaaaaaag gtagaaatca
    10801 ttcacaaaaa tgataaaaac gagcaaaaga ttaattttct cgaaaaaatg aaaacatttt
    10861 actatatttc atgaaaaaag tctgctataa ttagcagtcg agtaaaattt gccctgtcat
    10921 aaagaaccaa taaaaaagga gttagttaca ttgaattata gaaacgatat ccgtaacgtc
    10981 gcaattattg cccacgttga ccacggaaaa acaacattag ttgatgaact attaaaacaa
    11041 tcagatacat tagacgcaca tacgcaattg caagaacgtg cgatggactc aaatgcgcta
    11101 gaaaaagaac gtggtatcac gattttagcc aaaaacacag ctgtagacta taaaggtatt
    11161 cgcgtaaaca tcatggatac tccaggacac gcggacttcg gtggcgaagt agaacggatc
    11221 atgaaaatgg ttgacggcgt tgtgttggtc gtagatgcgt atgaaggaac aatgcctcaa
    11281 acacgttttg tattgaaaaa agcactggaa caacatatca caccaatcgt tgtcgtaaac
    11341 aagattgaca aaccttctgc tcgtcctgag catgtcgtag atgaagttct tgaactattc
    11401 atcgaattag gagcagatga tgatcagtta gactttcctg tgatttatgc atctgcacta
    11461 aatggtacgt caagcttgtc agatgatcca gcagatcaag aaccaaccat ggcacctatt
    11521 tttgatacaa ttattgaaaa aatccctgct cctgtcgata acagcgacga accattacaa
    11581 ttccaagttt cattacttga ctacaacgat tatgtaggtc gtatcggaat cggacgtgta
    11641 tttagaggaa caatcaaagt aggagaccaa gtagcattaa ttaaattaga cggaacagtt
    11701 aagaaattcc gtgtaacgaa attatttggt ttctttggac taaaacgtct agagattcaa
    11761 gaagcaaaag ctggagactt gattgctgtc tcaggtatgg aagatatctt tgtcggtgaa
    11821 acagttactc ctgtggatca tcaagatgcg ttacctatcc ttcacattga cgaaccaaca
    11881 ttgcaaatga ctttcttagt taataattca ccatttgccg gtcgagaagg taaattcgta
    11941 accgctcgta agattgaaga acgtttgatg gcagaacttc aaacagatgt atccttacga
    12001 gttgaaccaa caaactctcc agatgcatgg acagtttctg gtcgtggaga attgcatttg
    12061 tctatcttga ttgaaaacat gcgccgtgag ggatacgaat tgcaagtatc tcgtccagaa
    12121 gttattgaaa aagaaatcga cggtgtaaaa tgcgaaccgt ttgaacgcgt gcaaatcgat
    12181 acaccagaag aatatatggg tagtgttatc gagtccttga gcttacgtaa aggtgaaatg
    12241 caagatatgg tccatacagg aaacggacaa atccgtttga cattcttaac acctgcccgc
    12301 ggtttgattg gttattcaac tgaattcctt tcaatgacac gtggttacgg aatcatgaac
    12361 catacttttg accaatatct gccaatgctt cctggtcaaa ttggcggacg tcaccaaggg
    12421 gcgcttgttt cgatcgatac aggaaaagca acgacttatt ctatcatgag tatcgaagaa
    12481 cgcggtacag tcttcgtaga accaggtaca gaagtatatg aaggaatgat tatcggggaa
    12541 aacagccgtg ataatgattt gactgtcaac atcacgaaag caaaacaaat gacaaacgta
    12601 cggtctgcta ctaaagacca aacatctgtc atcaaaaaac caaaacaatt gacgttggaa
    12661 gaatctttgg aattcttgaa tgatgatgaa tattgcgaag taacaccaga aagcattcgc
    12721 ttgagaaaac aaattttaga gaaaaatgct cgtgaaaaag catctaaaaa gaaaaaataa
    12781 tctgtaaaag gatgaagaga gaaaccagct tgttataaaa aatagctggt ttcttttttt
    12841 gtccaactct tttctgtgga gaagtcatct ttgtcaatgg acataaattc agaaaatacg
    12901 ccgaaagacg tttaaaagac ttttaaaaaa cttatttcta gagagttttt gtttcataat
    12961 aagataatgt ttcacgaaag gagatcaact tgtccttcga agaaaatcga agtaaaatgg
    13021 caagagaaag gagcgggaag atgaacgact tattaaaaca atactttggt tatgatgagt
    13081 ttagacctgg tcaaaaagaa attatccaga aagtcattga tcaggaaaat gtattaggaa
    13141 tcatgcctac cggaagtggg aaatcaatct gttaccagct tccagcttta ctattagatg
    13201 ggcttactgt ggttgtgtcc cctttaatct ctttgatgaa ggatcaggtg gacgcggcga
    13261 atcaattagg tatacccgcc acttttatca atagctcgtt agatggttac gaaacagcta
    13321 gacgcttcca agaaatcgat agacagcagt accgtttact ttatatcgct cctgaacgtt
    13381 ttatcatgcc ggattttatc caagcaatga agcgatggaa cgtccgtatg atcgctattg
    13441 acgaggcaca ttgtatctct cagtgggggc atgatttccg cccaagttat ttacagatgg
    13501 cgaaccagct agatcaactg ccaaatcgtc ctgtaattgt tgctttgaca gctacagcga
    13561 ctgtgcaagt agctgctgac ataaaaagac ttttaaaaat tccagaaaac aaccatattc
    13621 agactggttt tgaacgagaa aatctacgat ttcaagtaat taaagatcaa aaaaaagaac
    13681 aatatctaat cgaatattta aagatcaaca aaaatcaatc tggtatcatt tatgcagcaa
    13741 cgagaaaaga agtagaccgt ctctatcatt tgcttaaaaa attcgatttt tccgtgggta
    13801 gatatcatgg tggtctaaat gagaatgagc gaacagaaat gcaagaagcc tttttatacg
    13861 atcgcttgca gctgattgta gcgacaaatg catttggtat gggtataaat aaaagcaatg
    13921 tccgttttgt tatccattat caaattcctg gctctctaga agcatactat caggaagctg
    13981 gtcgggcggg aagagatggt ctttccagtg aagccatcct gcttttctct cctcaagata
    14041 tccaagtcca aaagtttttt atccagcagt cccaaagaga agaagggcaa aagcaaaaag
    14101 aatatgaaaa attaaaagcg atgacagaat acgtgtatat tgagtcctgt cttcagcaat
    14161 acatcctaaa ctattttggc gagacaagca gtccgtgcaa ccgttgtggt aactgtttag
    14221 atgacagaga aatagtagaa gtaacgacag aagcacagat ggttctttct tgcttaaaac
    14281 gtatgggtga gaactatggt aaacaaatgc tgatgaaagt cttagcagga tcgaaagaac
    14341 aaaagctgca agctttaggt tttggacatt tatctaccta tggactgatg aaaaaccaat
    14401 cgcaaaaaga aacgatgcag ttaattgaat atttgatatc gaatggctat cttctgacca
    14461 tcaatggtga gtatcctgtt ttgaaagtaa cagaaagagg gatacaagta ttgaaaggac
    14521 aagaatctgt ttatcgaaag gaacccaaga aagtccagca gctatcggat gaagaaaccg
    14581 atacactatt tgaagtgctg cgggaactgc gaacggatct agcatcagaa gcaggcgttc
    14641 ccccgtatgt cgtattttca gattccacat tgaaagaaat gagccgaata cgtccatcaa
    14701 gcagattgga gatgcttcag ataaaaggag ttggccaaag caagcttgat aaatatggcg
    14761 aagccttttt atctcggata aaaaatgcgg actctaacgt tctggataag tagaaagaac
    14821 gaatacgtaa aaatgaataa aaacaactgg ttaaatctgt tttttttgct ccttatgaaa
    14881 atcaaatatg ttataattaa ggaattagaa tgggaggtaa gcacagatgg attctttgtc
    14941 gaaaaaactt gcgatccaag tattaaatga tgaagcagaa cggatcaaac gattaatccg
    15001 aaatcaaaac aacagtctct gtatctcaca atgcaaagcc ttcgaagaag tggtagatac
    15061 acagatgtac ggcttttccc ggcaagtcgg ttttgctgtc cgtataggag taatcggaaa
    15121 gaacgagggc cagctgatgt taagtgagct agaacgagaa ttgaatcagt tatatagtga
    15181 ggtttaccaa gaaatttatg ataaaaaaga agttggcaag gaggaataga cttgcctaag
    15241 aaagtgaaaa aaaggcatct attagactat agcatcttga ttccctattt aattttatgt
    15301 gtattgggat tgatcatggt atatagttct acgtcgtatc tattattaga aaacggacaa
    15361 aatccgtccg catcagtcat taaccagagt attttttggg tgcttagctt gattgctatc
    15421 gcactacttt ataaaatgaa aacagatgtt ttgaaaaacc agcgtttgat catggcagcc
    15481 atcgcagttt tgacgatttt gctgctgatt gttgtgtttt ttggtaagga gatcaatggg
    15541 gcgaaaggtt ggctgcaaat tgcaggattc tcgatccaac ctgccgaata cctaaaaatc
    15601 atttctatct ggtacctgtc tctgacttta tctaaaagac aaaacagtgt acaaaaagat
    15661 tttcttggga ctgtgaaacg tccacttgca atggttatcg gacttactgc attagtcgct
    15721 attcttcccg attttggtaa tgcggcagtt atctttttga ttattttggt tctgctttta
    15781 gccagcggag tcaattatgt gtatactttg attgtaggag taggaggctt ttgtctaagt
    15841 acttttacga tctggctgat caatatcaca aacggaaaga tcttacccgg acgtttacag
    15901 tatatctata atcgatttgc tatttaccaa aatcctttct cagacgaatt aaataaaggg
    15961 catcaactag tcaacggcta ctatgccatg ttcaacggag gactatttgg caggggatta
    16021 ggtaacagta tccagaaaaa aggattttta caagaagcac aaaccgattt tatttatgca
    16081 atcgtagtgg aagaactggg agtgattatg gggattttga tattagcgtt gctattcttt
    16141 atgattgtgc gcattgtcct tgtaggtatc cgttcaaaag atccgttcaa ttcgcttttg
    16201 tgcattggga tcggcgcaat gtttttgatc caagtatttg tcaatcttgg aggaattact
    16261 ggcgttattc ctttgacagg gatcacgttc ccatttttaa gtcaaggagg ctccagtttg
    16321 ctgatgcttt cgatttgtgt cggctttgtc ctcaacatca gtgcggatga aaaacgcaag
    16381 agcttaggtt tataataaaa aaagatgtag gagtggttcg atgaaaaaag ttttagttgc
    16441 taacaggggt gaaatcgctg tacgtgtatt tcgtgcttgt acagaattag gaattaaaac
    16501 tgttggaatc tatgcagaag aagatgaata ttccgtacac cgtttcaaag cggatgaagc
    16561 atatttagta ggacaaggaa aaaaaccgat tgatgcgtat ctagatattg aagggatcat
    16621 ttctatcgcg aaagaatgcg gagctgacgc tattcatccg ggttacggtc ttttgtcgga
    16681 aaacttgaat tttgcgcaac ggtgcgaaga agaaggcatc atttttgttg gacccaaact
    16741 gcatcattta gatatttttg gagataaaat caaagcaaaa gctgcagcta tcgaagcggg
    16801 gattgcttcg attccaggca cagatggacc aatcgcttct atagacgatg ccttggagtt
    16861 tgcgaaacaa tacggttatc cgattatgat caaggcagct ctaggcggcg gcggacgcgg
    16921 catgcgtgta gctcacgatg aaaagagtgc aagagagggc tacgaacgtg cgaaaagcga
    16981 agcaaaagca gcctttggta gtgatgaagt gtatgttgaa aaatacatcg ctaatcctaa
    17041 gcatatcgaa gtacagattc tcggggatac gcatggaaac gtcatccatc tttttgaaag
    17101 agattgttct gttcagcgcc gtcaccaaaa agtagtagaa gtagcgcctt gtgtctctat
    17161 gaatgaacag caacgtcaaa aaatctgcca ggcagctgtc caattgatga aatatgtcgg
    17221 gtatgtcaac gcaggtacag tagaatttct tgttgaagga gatgattttt actttatcga
    17281 agtcaaccct cgcgtccaag tggaacatac gatcacagaa atgattacag atattgatat
    17341 cgtaacaacg caactgttga ttgctcaagg tttggatctg cataaagaaa tcggattgcc
    17401 tcagcaagaa ggaattaaat taaatggttc agccattcaa tgccgaatca ctacagaaga
    17461 cccgttgaat aactttttac cagacacagg gaaaatcgat acgtatcgtt cacctggtgg
    17521 ttttggcgta cgtctggatg tcggaaatgc ttacgcgggc tatgtagtga caccgtattt
    17581 tgattcattg ctagtaaaag tatgtacgca tggtgctacc tttgaaacag ccattcagaa
    17641 aatggaacgt tgcttgaaag aatttagaat ccgtggagtg aaaacaaaca ttccatttat
    17701 gctcaatgtc atcacacatc ctgaattcca atcaggaaat gcaaaaacaa ccttcattga
    17761 tagtacagca acattatttg aatttcctcg actacgagac cgcggaaaca aaacgatgaa
    17821 atatatcggt gaaattactg tgaatggttt tccaggtatc gaaagtggcg aaaaaccatt
    17881 ttatgaagaa ccacgtatgc ctaaagatct aatcacaaga actgattacg taacggctaa
    17941 gaatgtatta gatgcgaagg gtgccgatgc tttggtcgaa tggatcaaag gacaggaaaa
    18001 tttgttgttg acggatacga catttagaga cgcccatcaa agtctgctag caactcgtgt
    18061 tcggacaaag gatttcaagc agattgcacg tttgactgga gaaggtcttc ctgaactctt
    18121 ttcaagtgag atgtgggggg gagcaacatt tgatgtggct taccgctttt tgaatgaaga
    18181 tccttggcaa cggttgagaa aaattcgttc tttaatgcct aatacgttgc tgcaaatgct
    18241 tttcagaggt tcaaatgccg ttgggtattc taattatcct gacaatgtat tggtggaatt
    18301 tgtcaaagaa gcagcagcgc aaggaatcga tgtatttcga atctttgata gtttgaactg
    18361 gactccacaa atggaaaaaa gtatccaagc tgtacgggat actggtaaaa tcgcagaggc
    18421 agctatttgc tatactggcg atatcaacga tccaagtcgg gcaaaataca atgttcagta
    18481 ttataaagac atggcaaagg aactagaaca actgggtgcg catatcattg caatcaaaga
    18541 tatggctggg ctgcttaaac cgcaggcagc ttatcgtttg attagtgaat tgaaagaaac
    18601 gacagatctg ccaatccatc tgcatactca tgataccagc gggaatggaa tcatcactta
    18661 ttctgctgct tcaaaagcag gagtagacat cgttgacgtg gcaatgagtg caatgagtgg
    18721 gaatactagt cagcctagca tgagcagctt gtattatgca ctggtcaatg gtccacggct
    18781 gccagaaatc acgattgaaa atgcacagaa attaaaccat tattgggaag atgtgcgcat
    18841 gtattacaaa ccgtttgaaa acggcttgaa tgctcctgaa acagaagtat atatgcatga
    18901 aatgccagga ggacagtatt cgaatctaca gcaacaggca aaagctgtcg gattaggtca
    18961 tcgctgggat gaaatcaaac aaatgtatca tactgtcaac ttgatgtttg gcgatatcgt
    19021 taaagtaact ccatcatcta aagtagttgg agatatggca ctatttatgg tccaaaatga
    19081 tcttacagaa gaagatattt atgaaaaagg cgaaacacta agtttccctg aatctgttgt
    19141 gactttcttc caaggagaac tagggcaacc cgttggcgga tttcctaaaa aattgcaaaa
    19201 gattattctt aaaggcagac ctgcattgaa tgaacgtcca ggtcttttgg ctgaatcagt
    19261 cgacttcaac gaagtaaaaa aagaattggc agaaaaaatt ggctatgaac caaaacaaga
    19321 cgaagtatta agctatttga tgtatccgca agtctttttg gactatcaaa aagcatataa
    19381 tcaatttggc gatgttacat tactggacac accaacattt ttccaaggta tacgtttggg
    19441 tgaaacaatc aatgtacaaa tcgaacgcgg aaaaacgctt atcattcgtt tagatgaaat
    19501 cggagaaccc gatatcgaag ggaatcgcgt actgttcttc aatttgaatg gacaaagacg
    19561 tgaaatcact atcaaagata attcgatcat cagtacggtc caaacccgat tgaaagctga
    19621 accgacaaac cgagaacaaa ttggagcaac gatgtctggt tctgttttgg atgtattagt
    19681 gaaaaaaggc gataacgtta aaaaaggtga tacgttgatg attactgaag cgatgaaaat
    19741 ggaaacggcc atcgaagcac gttttgacgg agaagtcgca catgtatatg tgtcttcagg
    19801 agacactatt tcgtctggtg accttttgat tgaagtgact gagaaataat aagcgaaagg
    19861 tggggaaaca tgaaaagact gggtttgttt tggctgattc tgctattagt tattgtggca
    19921 ttctacatgg aaccagtctt gtttccgcca aaacttgaag tgaaggaaaa ctctgatcaa
    19981 caaatatcaa accaaatgac aacgtggaaa taccaggagc ttaaagcaac tggtttttct
    20041 acttatatcg gaaaaccgat cgatcagctg gaaaaggatt ttggccaagc ctatgatcga
    20101 ttaactagcg gatttggttt tgaaacaaga tattatgaaa ataaagctag tcatctcaca
    20161 tttgaagcga acatccaaga cagcagagtc acaactgtta aagtactgca aacagggcct
    20221 gctgatatcg cgccgtttta tttagggatg acgatgcaag atcttacgaa aatcactact
    20281 atctatacta atttcacttt tgagtaccat gatacagaag ttggaatcga actaatggaa
    20341 gaggatatga attatcgtcc gctaattgct tttgataacg acacttttgc tattttattt
    20401 tttgatcaaa caacaaacgg cctattttca gttgtgtatt tagataaaga tagtcttttg
    20461 aaactattgc catatcaagt gtttggtgaa ggccttccac attatcaagc agataaatcg
    20521 gctgattggg aatcaatcaa tcgcgttaaa gaacgaaaaa gtacgacttt attaaatatg
    20581 cttagaagag aagacgaact tcccatatac aatcaaacac ttgcctttca aaaccacagt
    20641 cagctgctac tacatgattt tttgacaagg cctgaggaaa tattgtcaga ggaacgtatg
    20701 gctgattggc aaaaatcgct aggcagtgct caaacgacag tgaaattctc actgacgaat
    20761 gatgaattgg agaagttaat cagtaaacaa aaattagaaa atactagtgg gctatttatt
    20821 catccggcga ttgacgctac tttttcgttt ttatattggt acagcgatcc ttacaaccat
    20881 gaccgtttca tggacccggg aacggattct gtaggcattg ctttttctaa agaaaatatg
    20941 ttagtcttat tgcaagaaga aaacggagaa agtagtgata gcagtgatcg ttaacgaaga
    21001 gctgtttcaa ctggaggatc aatgcctcca agtcgctgaa atgataaaaa agagtaaaac
    21061 gatggcttct tatcttgaac ataaacagtc aatggaagaa tctgcaaaag tttgccagtt
    21121 gaaagaagcg ttcttgtcta agaaagaagc atttgaaaat gtagcggcat atggtgagta
    21181 tgcacctgat tatcgtgaaa aaaaacgagc tgttcgaaaa gcaaaaagac agcttgatat
    21241 ggatgagcat gttgcagctt tccgatatag tgaaacacag ctgcaaagtc tgttggatca
    21301 aattgtccaa gtacttgccg aagaagtttc taatgaaatc aaagtagatg caggtagccc
    21361 gttttttgaa acgaaaaaac actttggatg cggaggtaat tgtcatgcaa ttagacaata
    21421 gtgaatttga aataacaaaa cgacgcgggc tgatcgtatg ggtctacagc ttaaaacaat
    21481 taaagaatct gaaaaagttc ggcttgttgc attatgtctc aagaagaatg aaatatgtcg
    21541 tgctttactt aaatgaagag acattggaag aaacagaaga acgaatccgt aaattacatt
    21601 ttgttcgaaa cgtagaacgt tcttaccgac cggatatcga aatgaatttt gccgaaaaaa
    21661 taggaaacaa aacagtatta aaagacgaag gtttcgaaat tgaagagcta aatacagaaa
    21721 tccgattagc tgaacatctt taaaaatctc ttatgagcag tttttaaata aaaaataact
    21781 tggataacga tcgatttttg atcattgtcc aagtattttt ttggtatact agtaccagta
    21841 ccatttctaa ggagtgagta gatgcggata atatcaggaa attatggcgg gcgccgttta
    21901 aaaagtttag caggggcgaa caccagacca actacagata aagtaaaaga atccatattc
    21961 aatatgattg gaccatattt tgatggagag acagtattgg atctgtttgc aggaagcggt
    22021 ggtttagcta tcgaagcagt atctagaggc tgcagtcatg cggtatgcgt agacaaaaat
    22081 taccaagcgc taaaaatcat caaagaaaac atcgaaatta cgaaagaacc agagaaattt
    22141 accacaataa aacttgatgc ggacaaagca atcgaacggc tagcagcgga gaaacaacaa
    22201 ttcgactact tgtttcttga tccgccatat gcaaaacaaa aaataatcga tcaaatccat
    22261 aaaatggaag aactgggact tttttctaaa accgcagtta tcgtatgcga aacggataaa
    22321 acagtcgaat tgccagaaac tgtggctgga ttccatcaaa taagaaaaca gatttatggc
    22381 ataacggccg taactatcta tagaaaagag ggaaatcaat gaaacgtgca ttattcccag
    22441 gaagttttga tccttttaca aaaggtcact tagatacggt ggaacgtgcg gctaaattat
    22501 tcgatgaggt agtaattggt gtttttatca atacttcaaa gaaaagtttg tttcccccag
    22561 aagaaagaat gacattgatc acaaaagctg tttctcatct tcctaatgta aaagtcatgc
    22621 atcaagaaaa tcaattgacg gtagaaacag caaaagagat tggtgcagat gcgttgatca
    22681 gaggcatacg aagtatcaaa gactttgaat atgaacgaga gattgctcag atgaatcatc
    22741 atctatatga tgaattggaa actgtcttct tattagcaaa accagaatat agtcacgtga
    22801 gttccagtat tttaaaagaa gtactgcact ttggcggaga tgtgagcagc tacttaccgc
    22861 cagtgatcaa cgaagcttta gctggaaagc gtgaatcaaa tgaaagctaa acaatggttg
    22921 aaaacagtac tgattatcat atttggaatg ttgatcattg gatttgtagt agttcccttg
    22981 ccgtattatg tagaatcacc gggagccacg atcgatctga aagaactaat tacggtcaat
    23041 gataaggaag ataaaggttc tgggtctttc tctcttacct ctgttggtat caggcaagct
    23101 acaggtttct tagctttaaa agctaaaatg accccttttg aagaattgat cagtgaagaa
    23161 gaattgactg gtggggcgac aaatgaagaa tataatcaaa tgcaacagta ttatatggat
    23221 tcttcccaaa atgcagcaat cgaacaagcg ttaaaacttg cagacaagcc ttataaaatg
    23281 gaattcaaag gcgtatatgt catgagtgta gagaaaacat cgaatttctt tggcaagctt
    23341 tcagttggag atacagtaac aaaagtagac gggaaaagct ttcaaagtac agaagcattt
    23401 atggattatg taaaaagcca aaaagttgga caaacaatcg aaatcagtta tttgcataat
    23461 ggtgaagaaa aaacagcttc tggtgatctg atcgagttac caacagataa aaaggcagga
    23521 ataggtatca cgcttactga ccataccgag atcgaatcag atacagatgt tcgtattaat
    23581 tctggctcta tcggtgggcc atcagcggga ttgatgttca ctttagaaat ctatgaacaa
    23641 gtcactggta aaaacttgcg acatggtaag caaattgcag gaactggaac gatcaacagt
    23701 gaaggagaag ttggccgaat cggaggaatc gataaaaaag tggcaagtgc ggataaagct
    23761 ggcgtagaga ttttctttgc accagatgat acgatttcag acgatgttaa gaaagaatat
    23821 cctgaaataa aaaccaacta cgaagaagca aaagctgccg ctaaaaaatt gaattcctca
    23881 atgaaaatcg taccagtaaa aacagtacaa gaagcgcttg actatctaga gaaaatgaaa
    23941 tgaaactaaa taattaaaga ggggttgtga cacgagttct gtcacaaccc ctctttttcg
    24001 tcaaaaatct tcacgataaa aataaaaaca aaagaaaaaa ttgacgtaat tagatgttga
    24061 agggacgatt ttgccagagc tcactgtttc ttcaatttta aatgaaagga gtaatgaatg
    24121 atttttactg gtcattcttt gaaaaaagaa caatgataga aaaaatcagc aagaaaatac
    24181 tagcttcacc caaattgatc atttggatgt ctatgatagt tcttatcttt tgtatcgcaa
    24241 gtggtttttt attatcaaat ttgctatttt atagagaaac agaagagact gctgcaaaag
    24301 aaatactttc ttcgtttagt tccagtggag aagatccatc agctgaagga aacgatgtag
    24361 aagaggatgc tcgtccaaag gtcatgtaca ccgatatcaa aggaagtgtg aaggagcctg
    24421 gtatctattc cttttcttca gaagaacggg tctatgatgt actaaagcgt gcaggtggat
    24481 tattagaaga agcggatagc gatcgaatca acttttctgc gaagatagaa gatcagcaag
    24541 tcctatatat ccctgctgta ggtgaagagc tgccagaaca tcttgatcag ttggcatcct
    24601 cggaaggaaa acagcctaca gcagattccg agcctcccaa aataaatatc aatacagcca
    24661 ctccatcaga acttcagcag attcctggga tcggatcagt caaagcgcaa gaaatcattc
    24721 gatttagaga agaaaacgga tccttccaga aagtggaaga cctgcaggag atttctggta
    24781 tcggtgaaaa gacagttgaa aaactaaaaa acttcgttac aattaaatag aatgattcaa
    24841 aatcgactgg cacgagcaat aaatggacgc taatggatac taagaataat ccaattctaa
    24901 gagcttggca ggacgaggag gtagtatttt tggataggaa atagcttgat tgttcgcaaa
    24961 gaaacattaa aaaaaccaat gctgaatgtc tatctccaaa ataaaatatc tgggattcat
    25021 attatgaata cggctgtcag cggcaataac agtcaagctc tacgagaacg ctttgccaaa
    25081 gatgtactta gctatacagc agataaagta tttattctca ttgggacgaa tgatctagca
    25141 gagcataaac aactttctaa agaaacgtac caaaaaatct gcagtggatg atagttcagc
    25201 tgaaaaaaga ctatagttct gatcatgctt tctttatcac gccaccagca gtcgatgaga
    25261 caaaacaaaa atatcggaat aacaagttga tcaaagaata tatctctgtt ctcacagaag
    25321 tagcaaaacg ccatgattgt gaagtatcga tttttatacg gaatttcttg aacaaatgga
    25381 ttcgaagaat ttttataatg ggctaatgga tgacggactt catttcggca gtgaagggta
    25441 ggatcttttg tcagacagga tgattcgtta tatttaaaga atattttgtt taagcaatga
    25501 gtacttttta atcaacttga aaaaacggag gaatataaaa catgttagaa aaatcttcaa
    25561 aagcatgcga tacgaataaa gaaaaagtta ctgataagtc acataatgaa cgcattcctt
    25621 gggaccagta ctttatggct caggcagtac tgttatcttt acgtagtacg tgtacacgtt
    25681 tagaagtagg agcgacactc gttcgagaaa aaaggatcat tgctggcggc tataatggag
    25741 cagtcagtgg agatgtccac tgcatagatg aagggtgtta cgtcgtggat ggacactgct
    25801 tgcgaacgat ccatgcagag atgaatgcac tcctacagtg tgcaaaactt ggtatcccga
    25861 cggaaggttc agaaatctat gtcacacatt ttccatgttt ggcctgtaca aaggctttgt
    25921 tgcaagctgg tgtgaaaggg atcaattatc tccatgacta tcgaaatgat ccttatgccc
    25981 aagcgttgat cgaacaatta ggtgtatctg tacaccaagt tgctttagac tcacagcatt
    26041 tttcaaaact tcaaaatgaa ttaacaggaa aacatgtcta gcgttttaga aaggcatgtt
    26101 cgaaatcggt tgatttttcc ggcaattacg tcgatgatta tctgtttgtt gtattacact
    26161 gacaattggg gttatcgttt gcttcttgcc ggaatttttt tatggttttc gctaaaagaa
    26221 ggacgtttta tcgcgtactt gtgtattttg tgtagcataa cggtatccat ttcttatagg
    26281 ctacaagagt attcattttt agaggaaaaa acaacatctg cgcaaattac tgtatttaca
    26341 gatacgattc gtgcaaatgg taattttttg acaatggaag gaaggttatc agagaataaa
    26401 caaaagctcc agctttctta tcaagtaaac acagaagcag aattgaaaac atggcttaac
    26461 ttccgaggaa agggaataga attgactgct gaaggcgagt tcatctctcc agcaaaaaag
    26521 cggaatcttt ataattttga ccaagaagct tacttcagga accatagaat ttctggacgg
    26581 ttccaaattc aaaagattga acagatcacc atgcaaaatc actggcgaaa ctggcttcag
    26641 agaaaaagag gatcttttat ctcctttgtc caatctgaat ttccgcaaaa aacaagtgta
    26701 tatattaatt ctttattatg gggctataaa gatgcaggat tcagagaacc agaagaaatt
    26761 tttagggaga gtggtttgct gcatctgttc agtctatcgg gtctacacat ccaattttat
    26821 ttagggtggc tgtattattt atttcgacgt atcggatggc cactacacgt cagtattatt
    26881 ccgttgagtc tactaactat ctgttatgtt tctattgcag gaagttctat ttctgttttg
    26941 cgtgctagtt tgctgttttt attgcgattg ctagtcaaat tgctgaataa gcattattcc
    27001 accatggact tatttgcagt tgtcttttgg cttcttttac tatttgatcc tcgatttctt
    27061 tttcatgcgg gtggacagct tagttttttc atgagttttt tgtttatcct gatttctttt
    27121 gatggagatt ggttgaaaaa aacggcctct cacgtcctgt ttactatgat cacgatgccg
    27181 ttgattgtat ggcattttta tgagtggcct attttaggta gtctttttac attactgatt
    27241 gcaccggtat tcaagtttct ttttcttcct ggtgtgttat ttctcacact cttcaatcgc
    27301 tggctgccgg aaactttact tttgctgata gaagagggat taatcttatt tgagcgaata
    27361 atcgattttt cttcagggtc gtctctcatt ataggacgtt tgccccttgt gctatctggg
    27421 atgtatattt tcggattact gatttgtatg gataggttga aagaacaacc tgtgaaacac
    27481 ttgttttatg ttggtctttt ttcttttatt tttttgactt tgccattttt acgcttttct
    27541 tcaacgatcg cttttatcga tgtcggtcaa ggagacagta tctttttgca aagtcctttt
    27601 cgaaaagaaa cgattttgat cgatactgga ggtcagttac actttgaaag agaaaagtgg
    27661 gcgcaaggta tgatacgtcc tcctgctgaa agaaatatcg ttccatatct aaaaggtcag
    27721 gggataagtg taatcgataa actcatcttg acacacgacg atacggatca tgtgggagaa
    27781 ttacccacgt tatctcagca ttttactatc aaaaaaattt atatcggttg gggcgctgga
    27841 cgaaatgagc cattacaaag gcatttatct gaagctcaaa aaaatggtac tgagatcatt
    27901 gaaatcaaac atggggacag gatcagcggt tattatgatc tttatgtatt gactcctttt
    27961 gaaaagggag agggaagaaa tgaagattcg attggtctat ggatggaata taacaatagg
    28021 cgctttttat ttttaggaga tttgaatcaa gcaatggaaa aacaattgct tttgatttat
    28081 cctaatctga aagccgatgt agtcaaatta ggtcatcacg gtagccgtac gtcaagtaac
    28141 acagattttc tttcccatat tgaagcaaaa catgggatta tatcctgcgg cgtaaataat
    28201 cgttatggtc acccgcacag cgaagtattg gagacactag aagaaaatca gataaaaaca
    28261 tggcgaacgg atcaacaagg aatgatcacc tatcgctggc atccagtttt tcatcctcat
    28321 gggctagtgg aaacaatgat agactagtaa ttctcatctg ctcatggtag gataattttt
    28381 gaaagtggtg agtaatttga gtatacaagc agagttacaa aaaatacgta aagaaaaact
    28441 tgcatcttgt tacttgattt taggaacaga aaagtttctc caagatcaag ttcggacaga
    28501 aattgtcaaa aaaatccaat tagagggcga agatgactta aattttttaa gttttgacat
    28561 ggaaaatgca agtttagatg aggtagtagc tgaagcagag acgttgccgt ttttcggaga
    28621 tcaacggtta gtattcgtag agaatcctta ttttttaaca ggtgaaaaag taaacaatgg
    28681 aatcgaacaa aatactgatt tattgacggc ttatttgaaa gaaccattag aatcgactgt
    28741 attagtattc tttgcgccat atgaaaagtt ggatgaacgg aaaaaagtaa cgaaacaatt
    28801 gaaaaaaaca gcaattatca tcaatgtcca acagttgaat gaaaaagaag tacgtcaata
    28861 tctgatgaat accttagaga attcctcaat caaaatggaa cgaagtgcaa ttgatttatt
    28921 cttgaggttg accgacttag atttatccaa aatgatgaga gagttacaga aattggtcct
    28981 ttatggacaa aatcaacagc aaatcacggt aagagaagta gaagaattag ttcctaaaac
    29041 gttagaacat aatatttttg acatgaccca atatatttta aaagggaaga cggaacaagc
    29101 tttgcgttta tacgaagatt tagttatgca aggggaagaa acaatcaaaa ttaatgcgat
    29161 tttgctttct caattacgtt tatttttaca gacaaaattt cttgtaaaga ttggttatca
    29221 gcaggcaaat attgctgaaa cattgaaaat ccatccatat cgtgtaaaac ttgccatgca
    29281 ggaagtaaaa aaattcgatg aacatttgct ggtacagttg tttgatcaat tagttgagat
    29341 ggattatcaa atcaaaacgg gacaaatcga aaaagaatta agttttcaac tattcgtttt
    29401 gcgcacaggg cagttgttaa gaaatcgata agaagaaaga cgagggcaaa gcatgcggga
    29461 aggtcgtttg tttttgtcct ttttttgaca aataaaaaaa tcggccgaag ccgattcttt
    29521 tatttagcga tttttttgct taaacgagat ttgtcgcggt tagctttgtt tttgtggatt
    29581 agaccttttg attcagccat atcaatagct ttaacagctt ctttatataa cgcatctacg
    29641 ttgtcagcac ctgaagctac agcatcttca aattttttga ttgcagtacg catagcgctt
    29701 gtttgagatg aatttttcac attagcgttt tcgcttgtac gaacacgttt gattgcagat
    29761 tcaatgtttg gcattagttt cacctccgat aacttaagtt cttacgcgtg ttaccgcata
    29821 attcaacatt cattattata cctaaaggca gcgtgctttg caatatgaac atgacaagtt
    29881 tttttcctta acagtctttt attaggattt tttttcagta aaaacggata gtacaagctg
    29941 aaaaaaatca aaaagacgct attgtgggag gcaatagcgt caaaccaaac tagattttta
    30001 tttatctatt taaacaaagt ttgaatggga atgtttggta ctaagttatt atataacaaa
    30061 gagaaccgtt tgcaaagttt tttttgaaaa gggatacgat tccctgttgt tctttgtttt
    30121 tctattaaga aactgcagtt attttataac cagtatcctt tgatcagtaa aggctattct
    30181 ggtgtaccga ttattttaat gtattcatcg atatttttta tcaacatttt ttttatgacc
    30241 aactggttcc gacggttcca tttgccactt acttccagca catatttatt taggggcaaa
    30301 cgtaagatat attttgccaa atagcgatct ttaagcaggc cattgatcac acggtcctca
    30361 agctgcaagt tgatcccagt cagcatcatt ggctgttctt gtaaaacgta cgtatcaatt
    30421 aaaagacctt caaaagtttc cacaagcgtc ttcctttctt ttcaaagtaa cctgaaaaca
    30481 ggcactaatt cttccatttt gatattcgtt gaagtggatg cgatcattct ttcttttgtc
    30541 cactgtaatc tagtataaca aaaaagagca gaagagccaa aaagaaaaaa gagcatcgaa
    30601 ttctatagga agtctcattg gcacggccta aattattaca aaacattgat taaaaaaagc
    30661 ccctgcatcg caggagcatc ccctatatcg ttttaatagg tcaatcaaaa agcagtatca
    30721 aacacatttt gtatcaaatc aggaaataat agataagcta agcccagaat caaaagtacc
    30781 gctagaagaa accagccgat tgttcctatg aaatacttta atttttcgaa cattgtatca
    30841 cctcgttgtc agtatatcac ggatcagtaa attgggggtg ttaagaatct cctaaaatag
    30901 acaaaatttg ttctattttc ctaaagcaaa tacttgtaag atacagtaag tgataccaga
    30961 agcaatgacc ggtccggcgg cgattcctct taagaagaca acacctaaaa tcgtgccgaa
    31021 aacaagtgca acagtgattt gtggatcaga agcaatcaat cctacaccct tggaagataa
    31081 gacagccact agcccaccgc aaaaaacagc tacccagcca ataggggatt taaaggcttc
    31141 taatagatca cgtagaccga tttctcctgt tgcgataggg attagaatag ccgctgtgat
    31201 gatcgtcacc ccaatattga ttcctttgcc ttgaagccaa gggaaaaagg aacgggaggc
    31261 tggtatgagc ttcataatca aaacaaaagc tgccgctatc atcagacttt gatttttagc
    31321 taaaattgct acaagtaaaa taattcctaa aaaaagccaa ctttccatga aatatattcc
    31381 tcactttctt cgaaacaatc ataacatgtt tttagaaagt tacaaaagta ggaattcatc
    31441 cttcaagacc tattcataag ctgttttttt cataaaataa acaaaatcat aaaaaaacac
    31501 tttgcttatt tgacagaaga cagccgcgct ttgtatagtg gacaagactt acattttttt
    31561 gaagaatcag aaagatcagg taaaggtctt agtaaacgca agccattcag aataaccaat
    31621 attgtgcttc cttcgtgtcc tacaactcca agaggtagat ttaaaaattg caggaagtta
    31681 gaagcaatca acaagagaat aacaagaatc gaaaaaataa tattttgttt gatgatccgc
    31741 cgcattttta aagatagccg atgactgtag acaaatttat ttaagtcgct tttcattaag
    31801 acgatgtctg cgatatccat agcggcatct gtcccttgcc ccatagcgac acctaaagaa
    31861 gcggctgcta aagcaggcgc atcattgact ccatcaccaa ccatcgcatt tacttggtaa
    31921 agttcattct cttttttgac tagttgtgtt ttttcttccg gagtacagct tgcatagtaa
    31981 tgttcaatcc ctacttgttc agcaatggcc tttgctgtgc cagaatggtc gccggttatc
    32041 atacttgtat ggatgttttg ggatttaaag taggaaatta cttgttgggt atttgctttt
    32101 ggaatatcta gcagacctaa aacagcaatt aactgctgat ctttagaaag ataaatcacg
    32161 gttttgcctt gattttccaa acgttcgatc atttccgcaa tctctttgct gattatcatc
    32221 gtctcaggat catatgcgtg cttgccaact ttccattgcg cacctaaata ggtagtttgt
    32281 aatccaaaac cagtaatttc ttctattggt aagtattgga tttcttcagg tattgtgcaa
    32341 tcgaaatatt gcaaaatcgc atgagcaagt ggatgcgtcg ttttttgttc catagcaaca
    32401 agaagttgtt gtgccaaatc cttttcatca aagaaaaagc tgtcagttac aactggtttt
    32461 cctttagtga gcgttcctgt tttatcaaag gcaatcgctt ttaattcaga aagcttttct
    32521 aaatggatac cgcctttgat taaaatccca ttttttgcag aatttgaaag agcagctaga
    32581 gaagctggtg tagcagatgc gacaagcgca caaggagaag ccacaactaa taataccatt
    32641 cctcgataaa agctttcatc ccaactccaa cctaggaaaa agtgggggag aaggatcatt
    32701 agaggaacag caagcaaaat caattttaca taattatttt cgattcgttc gataaatgaa
    32761 gctgtttgag ttggcgtatt ctgcgcttct tctactaatt ggataatctt agcaaagaca
    32821 gtttcggaac tcgtttttgt tactcgaatc gtgatgggat ttccttgatt cagcgttcct
    32881 gcatacatct catcgcctaa atatttttct acaggcacag attcaccgct taatgcagac
    32941 tcatcgatcg ttgcctgtgt gctttctaaa aagccatcta tcggaacagc tgctccttta
    33001 gcgacaaata ccaagttatc cacctttagt tgagaaacat ctacttcttg catttgtcca
    33061 ttttctttta ataattgggc tttttgtggt tgaagattca tcaaagcagt aatttctttt
    33121 tgacttttat tagttgtgta ttcctctaat gcaccgctta aacaaaagat gaatgtcaac
    33181 attgctcctt caaagtaatt accgatcaaa caggcaccaa tagcagctaa agccatcaat
    33241 aaatccacat tcagtgtccg atcattccac aattctttca agccttccga tgtttgttta
    33301 tatccaccga tgataatggc actgatataa aaaagtgggg tgatggtaag agagaatcgg
    33361 tcgaagataa aaccgaagat cataaataaa aggcagccga ttgttgctgc aaaagctttt
    33421 ttctgattag tcatcgttat tagacctcct tgtcaaaaca aggatagcat aataaaaatg
    33481 taattgataa taataacagc taaatgagaa cgataatggt tatcatttgg tgattaagct
    33541 tgatgttgtt tttattattt ataatgatta ttattaaaga aaataaaaaa ccagaacatt
    33601 tctcaaataa ga
//
LOCUS       DACEEZ010000021        33568 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20313_2017
            SAMN12639966-rid9879223.denovo.021, whole genome shotgun sequence.
ACCESSION   DACEEZ010000021 DACEEZ010000000
VERSION     DACEEZ010000021.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639966
            Sequence Read Archive: SRR10023158
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 33568)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 33568)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741683.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741683.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:44:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,016
            CDSs (total)                      :: 2,962
            Genes (coding)                    :: 2,889
            CDSs (with protein)               :: 2,889
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 36 of 73
            Pseudo Genes (incomplete)         :: 29 of 73
            Pseudo Genes (internal stop)      :: 18 of 73
            Pseudo Genes (multiple problems)  :: 8 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..33568
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639966-rid9879223.denovo.021"
                     /isolate="E20313_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(21..977)
                     /locus_tag="IWO89_001080"
     CDS             complement(21..977)
                     /locus_tag="IWO89_001080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HAQ0455851.1"
                     /translation="MKKYQWGIVGLGTIAHEFAESFNQETSELTAVASRTSEKAENFA
                     HRYNIPKAYGSYQEMLDDAEIDIVYIAVPNRQHIDHILAALEAGKHVLCEKAITMNKK
                     ELADAMRLAEEKNLILAEAMTIFNMPLYQQLRSIMDTGKLGALKMIQAPFGSYKEPDP
                     KNRFFNPELAGGALLDIGTYAVSFARFFLSSQPEVVASTMVPFETGVDEQSVTILRNK
                     ENELAAVSLTFQAKMPKVGIVAFENGYVTIADYPRADRAEILFTDGTKEFIESGNTSE
                     AMNYEIKNMVHMIEGDLPNRSLFLTKDVIEILDQMQVLWKNM"
     gene            1151..1711
                     /locus_tag="IWO89_001081"
     CDS             1151..1711
                     /locus_tag="IWO89_001081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0455852.1"
                     /translation="MHQVYIDLIMSVLLEQFETEKNFLDEYLLISPEQWQKWKDGEKN
                     LPSEAMQKVKNLFTDYEWMLTQKILRQTIIFPEKRNVAVAEYKQIKTRIAQKWLSSKA
                     AKVELIPLKNNQQAGRYLDLKVSIQYDEWGYDDILNFRLPADVQKQIEGEKIELLDWV
                     NENLEETYVHEANGEREEQRKDFSSE"
     gene            1704..2528
                     /locus_tag="IWO89_001082"
     CDS             1704..2528
                     /locus_tag="IWO89_001082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M15 family metallopeptidase"
                     /protein_id="HAQ0455853.1"
                     /translation="MNKFLGFGAVVIMIAAMGYVIFSDDDSHVQAGNQAVERTSTKTT
                     ASKEKNSQTTSSKVKDKETKDRTSKQLPDVKTDDWKLVLVGPDHKLEKEIDESTQLAS
                     LSNGYLIDKRIAPEYEAFAKAAEAAGFPLVMVSAYRSVSSQQQVFEQNVQDVMSRQGV
                     SEEEATKITKETITEPGYSEHHTGLAVDVVDQNWYNSYPSTVLDASYGDQPGAKWIAD
                     NAAQYGFIVRYPKGHEDITKITYEPWHLRYVGKENAEYITKHQLTLEEYLNELNEK"
     gene            2538..3155
                     /locus_tag="IWO89_001083"
     CDS             2538..3155
                     /locus_tag="IWO89_001083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 73 protein"
                     /protein_id="HAQ0455854.1"
                     /translation="MAKKKWKKQKRKNIRWPAVVAGVAVILLAFVFSLKNLSSPFTDN
                     QQENEAQSHQQFIERLVPHAQELQDGYGVLPSIILGQAILESNWGKSTLASKYNNLFG
                     IKAYGDQKKVSLETKEFVNEEWITIQGDFKVYDSWEQSMDDHTQLFVQGVDWNPALYE
                     KVITATNYQEAAQALQDAGYATDPGYAQKIIQVIETYQLNQYDRR"
     gene            3173..3856
                     /locus_tag="IWO89_001084"
     CDS             3173..3856
                     /locus_tag="IWO89_001084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HAQ0455855.1"
                     /translation="MAEQFTPEIMTELRKDIVRVPEIILEASGIKIFGKLIRSIIFTT
                     DIAIIRNTNANAVIAVYPFTPHPAITKSIIEAADIPVFSGVGGGLTQGARSSYMSMFA
                     EAQGSLGVVLNGPTPLETVKEVCRVVDIPVISTVTSKYTEIDEKLAAGISIINISAGK
                     ETAEAVRHFRKKYPELPIIATGGPTEESIAETIQAGANAITYTPPSSGELFSRKMEKY
                     RETEKKNHE"
     gene            4539..5774
                     /locus_tag="IWO89_001085"
                     /pseudo
     CDS             4539..5774
                     /locus_tag="IWO89_001085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738728.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter substrate-binding
                     protein"
     gene            5782..6669
                     /locus_tag="IWO89_001086"
     CDS             5782..6669
                     /locus_tag="IWO89_001086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAQ0455856.1"
                     /translation="MKKEKLKRFLDQDNVVGYVFASPFILGFLLITVIPMILSLYYSF
                     TDYRLGNPVSWIGLDNYIKMLSDARFLNSIRVTFLYVFASVPVKLIFGLLIAYLLTRN
                     IKGTTFFRSLYYVPSLIGGSISIALVWRELFSKNGLVNIFLERLGAPALSWFSDQKLV
                     MIPLVLMSAWQFGSAMIIFAAGIKEIPGSYYEAAEIDGANKWQTFYKITLPLLSPIIL
                     YNLVMQTIAAFMTFTQAFIITQGGPNDATNFYALYVYNQAFSFNSMGYASAISWIMLL
                     IMGAVTFLIFKSSKKWVVV"
     gene            6686..7522
                     /locus_tag="IWO89_001087"
     CDS             6686..7522
                     /locus_tag="IWO89_001087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HAQ0455857.1"
                     /translation="MNNTVTKWFQYGFILLLGLVMIYPVLWMIAGSFKTDQEIMSGSL
                     NLIPETFQWENYAKGWAGFAGISFFTFFRNSFVISAVSTIGTVLSSTCIAYALSRIDF
                     KGKRFWFVVMLVTMMIPAQVILIPQFIIYNRLNLVGTYVPLILPHFFGQAFFIYQIMQ
                     FMVSIPKELDESAIIDGCSKYSVFTKIIFPLLKPSIITTIIIQFYWKWDDFMGPLIYL
                     NKPRSYTVSIAIKLFADAGSSTTYASMFAMSTLSLLPVFLIFLFFNKYLVQGISTSGL
                     KG"
     gene            7525..8148
                     /locus_tag="IWO89_001088"
     CDS             7525..8148
                     /locus_tag="IWO89_001088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0455858.1"
                     /translation="MKKPIEEMFLYKLNYYVCRIFLTSLISNALFALVLGCLFTLGLV
                     LSARVVYLIYFLMMYPSYYLSVHYLAEKKVCWRKGKIKRMCFIITLFQLFVIIFAVDW
                     YLLVGKIGAWYLIPFLLCLLLFVSHSFFSTLIDICKEDKADSLVYLIKRNIWLLSKNV
                     KGFLWYLCLLYGLFWCIQPDFLLTALLYPGLPFVTVFCLKEKENDFK"
     gene            8145..9437
                     /locus_tag="IWO89_001089"
     CDS             8145..9437
                     /locus_tag="IWO89_001089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HAQ0455859.1"
                     /translation="MKKKYVQVGLGGRARFFYEAIASTYHQTCELVGFCDVSQTRMDY
                     ANRILVNKYGQKKVKTCMPSQFEQLLDDVRPAEVIITSVDRTHHDYIIRAMKKGYDVI
                     CEKPLTIDEEKAQEIITVQKQTGRSLRVTFNYRYAPYHTKIKELIESDVIGDVFSIHF
                     EWLLNTQHGADYFRRWHRNKHNSGGLLVHKSTHHFDLVNFWLNSRPQKVFAFGGLNFY
                     GMHNAEKRGVEIFYSRCYGSELAKADPFAIDLSANEELREMYLEAEKDSGYIRDRSVF
                     SDDISIEDTLGVTVKYETGAILTYSLNAYMPWEGFNVSFNGSKGRIEMKVIEKSYINA
                     GGDKKDEGAAVLQSIHVYPMFGEPYEVIVEQEAGGHGGGDPRLLDDLFGEEKEEDPWN
                     RAATHVDGILSILTGIAANHSIASGKAVVIDELVSLKE"
     regulatory      9796..9893
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00167"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="purine riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="guanine and/or adenine"
                     /db_xref="RFAM:RF00167"
     gene            10009..10590
                     /locus_tag="IWO89_001090"
     CDS             10009..10590
                     /locus_tag="IWO89_001090"
                     /EC_number="2.4.2.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine phosphoribosyltransferase"
                     /protein_id="HAQ0455860.1"
                     /translation="MKELVERIQKDGRILGEGVLKVDSFVTHQVDPVLMEQMGKRFAE
                     VFKEQGITKVVTIEASGIAPALFAARELDVPMIFARKAKSLTMDEELLTASVYSFTKQ
                     VTSTISISRKFLSSEDKVLIIDDFLANGQAAKGLIELCQQAGAQVEGIGIVIEKSFQD
                     GRQLLEEMGLRVVSLARIASLTNGQVEFLEEDA"
     gene            10593..11900
                     /locus_tag="IWO89_001091"
     CDS             10593..11900
                     /locus_tag="IWO89_001091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine permease"
                     /protein_id="HAQ0455861.1"
                     /translation="MEKETQNGKAAVLGLQHLLAMYAGAVAVPLLIGTGLGFNQEQMT
                     YLISIDIFMCGLATLLQLTVNRFFGIGLPVVLGCAIQAVAPLILIGTNEGVGAIYGSI
                     IASGIFVVLVSGVFSKIKKLFPPIVTGTVITVIGLTLIPVAIEKMGGGNATAADFGDK
                     TNLLLAFVTILLIVGVQMLAKGFIRSIAVLIGLVGGSILAAFMGMIDLSAIGDAPLFH
                     VPQPFYFGKPTFDVWSILLMIIISMVSMVESTGVYFALGDITGKNVGEEELKKGYRAE
                     GLAVILGGIFNTFPYTGFSQNVGLVQLSGIKTRKPIYFSAFFLIVLGLFPKIGALAQI
                     IPEPVLGGGMLVMFGMVAVQGIRMLLEVDFSNDKNLLIVAVSVGFGLGFNVTPTLFSQ
                     MPQTLQMFTGNGIVMSSITAIILNLIFNGLKKDEEILEEAFKK"
     gene            12193..12681
                     /gene="purE"
                     /locus_tag="IWO89_001092"
     CDS             12193..12681
                     /gene="purE"
                     /locus_tag="IWO89_001092"
                     /EC_number="4.1.1.21"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HAQ0455862.1"
                     /translation="MTAKISVIMGSTSDWETMKEACQILDEFGVSYEKKVVSAHRTPD
                     LMFEYAQNARKKGIKIIIAGAGGAAHLPGMVASKTTLPVIGVPVQSRTLNGLDSLLSI
                     VQMPGGVPVATTAIGKAGAINAGLLAIQMLSMYDLELEAKLAERRSMLAKTVIESSDQ
                     LG"
     gene            12665..13798
                     /gene="purK"
                     /locus_tag="IWO89_001093"
     CDS             12665..13798
                     /gene="purK"
                     /locus_tag="IWO89_001093"
                     /EC_number="6.3.4.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide
                     synthase"
                     /protein_id="HAQ0455863.1"
                     /translation="MINLVKPLLPGSTIGIVGGGQLGRMMAISAKEMGFKVGVLDPVS
                     DCPAAQVADWHIESVYDDTFALKELARRTDVITYEFENVSVEALNAILPMSFIPQGTD
                     LLAITQDRLLEKSFLETNNIVIAPYATIVSPTDIQDAIDGIGYPCVLKTTRGGYDGKG
                     QYVLKSRADLAPAMDLLREGTCVLEAWIPFEKEISIMVAGNGQGDFTTFPVVENIHHN
                     NILHETIAPAAIDQDVIEEAERIARVIAEAVSLSGVLGVEMFLTKTGGLYVNELAPRP
                     HNSGHYSIEACSMSQFDAHIRGICGWPLGEKVRLISNAVMVNVLGEQLLESYRLIENK
                     PDWQFHFYGKKQAKKGRKMGHITILTEDIQQTLEEIKQTNIWD"
     gene            13811..15106
                     /gene="purB"
                     /locus_tag="IWO89_001094"
     CDS             13811..15106
                     /gene="purB"
                     /locus_tag="IWO89_001094"
                     /EC_number="4.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HAQ0455864.1"
                     /translation="MLERYTRPEMGNIWTEENKYRAWLEVEILADEAWAELGEIPQED
                     VKKIREHASFDIDRILEIEKLTRHDVVAFTRAVSETLGEERKWVHYGLTSTDVVDTAY
                     GYLIKQANDILREDLKRFTEIIGEKAKEHKYTVMMGRTHGVHAEPTTFGLKLATWYSE
                     MKRNIERFEHAAKGVEAGKISGAVGTFANIPPFIEEYVCKQLGIRPQEISTQVLPRDL
                     HAEYFSAMALIATSIERFATEIRGLQKSETREVEEFFAKGQKGSSAMPHKRNPIGSEN
                     MAGLARVVRGHMVTAYENVTLWHERDISHSSAERIILPDTTILLNYMLNRFGNIVKNL
                     TVFPENMKRNMSATFGLIYSQRVMLKLIDKGMTREEAYDLVQPKTAYAWDNQVQFRPL
                     LEEDPTITSVLSKEEIDDAFDYHHHLKNVDVIFKRVGLE"
     gene            complement(15286..15663)
                     /locus_tag="IWO89_001095"
     CDS             complement(15286..15663)
                     /locus_tag="IWO89_001095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0455865.1"
                     /translation="MEDAIKQGQLFLSELTAEERSCLYNDLLSIKDVYLCNKDTSFYT
                     FRKIRRATKRCAECRKRTFLYQTEHKGELSISQIHEQKRFKNHYRIQILFNGQEMEYA
                     FLENLLQHLHNQHFSYNSQAIYA"
     gene            15919..16647
                     /locus_tag="IWO89_001096"
     CDS             15919..16647
                     /locus_tag="IWO89_001096"
                     /EC_number="6.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HAQ0455866.1"
                     /translation="MEKKELLYEGKAKKIYLTSDENLIWIEYLDQATALNGARKDQIS
                     GKGELNNQITSAIFRYLTQRGIKNHFIEQLSKTEQLAKKVQIIPLEVVIRNIAAGSFS
                     KRLGVPEGKEFIKPIIEFYYKDDQLDDPFINEEHIYFLQLAKPEEISELKAQALAINE
                     ILTELFAKVGLVLVDFKLEFGRSNGQILLADEVTPDTCRLWDERTNEHMDKDVYRRDL
                     GDLIPVYQEVYNRLSELTEEKERV"
     gene            16647..16901
                     /gene="purS"
                     /locus_tag="IWO89_001097"
     CDS             16647..16901
                     /gene="purS"
                     /locus_tag="IWO89_001097"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010758866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurS"
                     /protein_id="HAQ0455867.1"
                     /translation="MYFVKVYVTYKDSVLDPQGEAVKKAVHRMGYETIEDIRIGKYFE
                     IQLADAEEPIDTVIETICDKLLANVNMETYRYEIKKMEEV"
     gene            16903..17574
                     /gene="purQ"
                     /locus_tag="IWO89_001098"
     CDS             16903..17574
                     /gene="purQ"
                     /locus_tag="IWO89_001098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurQ"
                     /protein_id="HAQ0455868.1"
                     /translation="MKFAVIVFPGSNCDADMLWAVREIMGADAEFVRHDESSLEGFDG
                     VLLPGGFSYGDYLRCGAIARFSTIMEEVIRFAEEGKPVFGTCNGFQILTEAGLLPGVL
                     RRNESLHFICKTVPLKVNPKTAFTSEYEADEIIYLPVAHGEGNYFCDEETLQELKKNN
                     QIAFTYNDNINGSVENIAGITNKAGNVLGMMPHPERAVEDLLGSEDGKRFFASILKNF
                     GKVTV"
     gene            17575..19797
                     /gene="purL"
                     /locus_tag="IWO89_001099"
     CDS             17575..19797
                     /gene="purL"
                     /locus_tag="IWO89_001099"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurL"
                     /protein_id="HAQ0455869.1"
                     /translation="MKTLKEPTAEEIKKKRIYAEWGLTDEEYRMIEEDILGRMPNYTE
                     TGLFSVMWSEHCSYKNSKPVLRKFPTSGPHVLQGPGEGAGIVDIGDGLAVVFKAESHN
                     HPSAVEPYEGAATGVGGIIRDIFSMGARPIALLDSLRFGELDNPRTKYLLEEVVAGIG
                     GYGNCIGIPTVGGEIAFDPCYEGNPLVNAMCVGLIEHKHIQKGQAAGVGNSIMYVGAK
                     TGRDGIHGATFASEEFNQEEEQQRSAVQVGDPFMEKLLLEACLELILHHSDSLIGIQD
                     MGAAGLVSSSAEMASKAGSGLILTLDEVPQRETGMTPYEMMLSESQERMLICVKSGEE
                     EKIQELFQKYDLDAVTIGKVTDDGQYRLYHQGEEVANLPVDALAEDAPVYHKEMKEPA
                     RIVEFQQLAPYQPVIEEPGQVLLDLLQQPTIASKRSIYETYDSQVQTNTVVRPGSDAA
                     VMRVRGTNKALAMTTDCNARYLYLDPKIGGQIAVAEAARNIIASGGKPLAITDCLNYG
                     SPDKPEVFWELSTSADGIAAACETLGTPVISGNVSLYNETDGQAIYPTPMIGMVGLIE
                     DHKHITTQEFKKSGDLIYILGKTFADFDGSELQKMQLGRIEGVIRNFDLSIEKRNQEL
                     VLTAIQNGLIESAHDCSEGGLAIALAESAFKHQLGISVQFELSSAQLFAETQSRFVLT
                     VAPENKTRFEEMMGDAAVLAGKVTDEAIIEISATDGQIKIETAVARKCWEDAIVCLMK
                     "
     gene            19782..21221
                     /locus_tag="IWO89_001100"
     CDS             19782..21221
                     /locus_tag="IWO89_001100"
                     /EC_number="2.4.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010768528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HAQ0455870.1"
                     /translation="MSYEVKSLNEECGIFGVWGHPDAASVAYFGLHSLQHRGQEGAGI
                     VSNDRGKLKGHRDLGLLSEVFKDQRKLQQLTGEAAIGHVRYATAGNGSVDNIQPFLFK
                     FHDQSIGLAHNGNLINARSLRKELEENGAIFHSNSDTEILMHLIRRSEETDFLNKLKS
                     ALNEVKGGFAYLLMTETAMIAALDPNGFRPLAIGQMKNGAYVIASETCALEVIGARFI
                     RDVQPGEIVIINDEGIQIDCFTKDTQLSICSMEYIYFARPDSNIAGINVHTARKNMGR
                     NLAKESPVKADMVVGVPNSSLSAASGYAEASGIPYEIGLVKNQYIARTFIQPTPELRE
                     QGVRMKLSAVRGVVEGKKVIMVDDSIVRGTTSRRIVHLLKEAGAKEVHVRIASPPLKY
                     PCFYGIDIQTRKELIAANHTIDEIKECIGADSLGFLSEEGLIESIGLEKDAPYSGLCM
                     AYFNGDYPTPLYDYEEKYQESLKEKVSFF"
     gene            21253..22296
                     /locus_tag="IWO89_001101"
     CDS             21253..22296
                     /locus_tag="IWO89_001101"
                     /EC_number="6.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine cyclo-ligase"
                     /protein_id="HAQ0455871.1"
                     /translation="MANAYAKAGVDVEAGYEVVERIKKHVKKTERLGMMGAIGGFGGC
                     FDLSVFQLKEPVLVSGTDGVGTKLAVAIEADKHDTIGIDCVAMCVNDILAQGAEPLYF
                     LDYLATGKNDPLRLENVVKGVADGCVQGNMALIGGETAEMPGMYSGNDYDLAGFAVGI
                     AEKSQLLTGETINPGDVLLGIASSGIHSNGYSLVRKIFFEQQQFSLSSKLPELKKTLG
                     EELLEPTKIYVSSLLPLIKEKKIKGAAHITGGGFVENIPRMLPESMAAQIKLGSWPIL
                     PIFGCLQKYGEIPEMEMYEIFNMGIGMVLAVEESKVKEIVHEIEQAGEKVYEIGKVIA
                     KEKHRVVFIGEER"
     gene            22293..22871
                     /gene="purN"
                     /locus_tag="IWO89_001102"
     CDS             22293..22871
                     /gene="purN"
                     /locus_tag="IWO89_001102"
                     /EC_number="2.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005873397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide formyltransferase"
                     /protein_id="HAQ0455872.1"
                     /translation="MRIAVFASGNGSNFQALADYLSKKGLESSIDWLFCDQPEAYVLK
                     RATALSVPADCFSPKEFDSKKEYEEAILHKLKEKKIDLIVLAGYMRIIGPVLLKNYDK
                     RIINIHPSLLPAFPGLHGIRDAFEAGVKETGVTIHYIDQGVDTGPIIRQEKVRIEQED
                     TFDSLEEKIHRVEHRIYPEVISEIIENGGYLK"
     gene            22868..24403
                     /gene="purH"
                     /locus_tag="IWO89_001103"
     CDS             22868..24403
                     /gene="purH"
                     /locus_tag="IWO89_001103"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HAQ0455873.1"
                     /translation="MTRALISVSDKTGIVAFAQGLIEQGVEIISTGGTKHALDKAGIK
                     TLSIEEVTGFPEMMDGRVKTLHPKIHGGLLGRRDLPSHMEAMETENIQPIDFVCVNLY
                     PFKETIMKPDVEIAEAIENIDIGGPSMLRSAAKNHEFVTSVVDPSDYEEVLSELRQYG
                     HTSLSFRQKLAAKVFRHTAAYDALIAQYLTDWVEEKEPEKLTLTYERKQTLRYGENSH
                     QAAAFYQSALLEKYSIAAAKQLHGKELSYNNIRDADAALRIMKEYDEPTVVALKHMNP
                     CGIGTASTILEAWEAAYEADPVSIFGGIIVLNREVDLPTAEQMHKLFLEIIIAPSYTQ
                     EALELLQKKKNIRLLTVDFAQEENQNREETVSVLGGLLVQDQDLAIENEEEWKVVTKR
                     KPTPAEAKALAFAWRAVKHVKSNAIVLANERQTVGIGAGQMNRVGSVKIAIEQAQEKM
                     EGAVLASDAYFPMDDSVEYAAKHGIKAIIQPGGSIKDQDSIDMANKYDLAMVFTDVRH
                     FRH"
     gene            24419..25693
                     /gene="purD"
                     /locus_tag="IWO89_001104"
     CDS             24419..25693
                     /gene="purD"
                     /locus_tag="IWO89_001104"
                     /EC_number="6.3.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HAQ0455874.1"
                     /translation="MNILVIGAGGREHAISKKLLASPKVDTVYCAPGNPGMTKDGINC
                     VAISERDHSALIHFVKKEAIDWSIVGPEVPLLNGIVDDFMAEGLKIFGPTKAAALIEG
                     SKSFAKQLMDTYQIPTAKSRSFECYEKAAAYIKENGAPIVVKADGLAAGKGVVVATTV
                     KEALDAAEQMLNQHRFGDSSKKIVVEEFLAGEEFSLLAFVRNTKVYPMVISQDHKRAF
                     DNDKGPNTGGMGAYSPVPQISKKMIEKAVEEVLKPAALGMKELGRSFTGVLYAGLIAT
                     KEGPKVIEFNARFGDPETQVVLSRLKSDFAQVIDDLLENRTVELKWQQEGYNVGVVVA
                     AAGYPEEYETGMVLPDFLEEELSVYYAGVSSNNGKLVGAGGRLYLVEAYGETIKEAQK
                     KIYTALMDKTTDGTFYRTDIGEKAANSELIVK"
     gene            25815..26729
                     /locus_tag="IWO89_001105"
     CDS             25815..26729
                     /locus_tag="IWO89_001105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase family oxidoreductase"
                     /protein_id="HAQ0455875.1"
                     /translation="MKKIQLGTSDQKAAAVILGCMRINNAEDPVKVIETAYENGIDFF
                     DHADIYGNGECEEIFADALAKTSIKREDLFIQTKCGIVPGVMFDFSKKHIIESVEKSL
                     TRLKMDYVDALLLHRPDTLVEPEEVAEAFDELERSGKVRYFGVSNQKPMQIELLKKYV
                     RQPLVANQLQFGLKHTGMIDQGIHVNMTDEASIDHDGSVLDYSRLHDMTIQAWSPYQY
                     GFFEGVFIGNEKFPELNQTLEELAAKYQTTPTGLASAWILRHPANMQVIAGTMNVGRI
                     KEIAQASEINMSREDWYELYRAAGNILP"
     gene            complement(26862..28427)
                     /locus_tag="IWO89_001106"
     CDS             complement(26862..28427)
                     /locus_tag="IWO89_001106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HAQ0455876.1"
                     /translation="MYKVFIIEDEYLIRNNLREQITVLGSYYPITYVGEAGDGEMALS
                     SIIDLQPDIILTDIQMPFMDGLTFSKEARKLLPWTRLIFISGFDDFDYMKGAIQVQAD
                     DYLLKPIKSSELKQAIEKAVTALDLQQKETHTSSDLVFELKKNHFLNGLFKGSLTISE
                     TLKLAESFNRSLAGKKMTVLLAANQVSTDFEDYAHLSDYLKFLFRENQQVIFSAVSSR
                     FIKFLIVDSLEENVIETSYQVAQTLVHELEKGQKNNFSVAIGPVAERISELPASFEIT
                     QMILETASSLKEQIISYEDLIQEAKLPVDSVFYFDLSLDLSSLSKNEVSKYIEHLTKP
                     QGTPTRTRLYRLFVLTKLLQHVCLKEKKIPQEMVHCTSTSQRLAISADDSLYRSTVYM
                     FISFLTALPSQPSRNKYQPILNHALDFIKENFTDPDMSLTWVAQQVALSPSHFSTIFS
                     QSFNQTFIDYLTEQRIELAKKLLQQSDYRISDIAFEIGYNDPNYFSYLFKKKQGISPK
                     EYRTQLFLKNKNN"
     gene            complement(28420..29871)
                     /locus_tag="IWO89_001107"
     CDS             complement(28420..29871)
                     /locus_tag="IWO89_001107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HAQ0455877.1"
                     /translation="MTKNLSIKQIFAQANKAMLLFAVIPLLLSILLYSQQLLIYQQTI
                     ANIQKADAIALKVDQKVLESMWDLISGQVTVDEFNEENILEELRNDIREIKNNTNTLK
                     EISTLDVSLRTIDTLEDYQNELINNILAKDFMDTNEEIINQVDSVTQLLSDILQDFVR
                     VEIDLASKKNTTLVRSLILLTIVELGVLSTIFFYAKKNRRFLERKVQEPINQLIEMSE
                     QLSSGNLGFRLTLPETLELQTLTASMNKMAEDLNQSLRENALKQYHLAQSEVRVLQAQ
                     ITPHFIYNSLDAIISLIEQDCFEEALTMTHALSGFFRTSLSKGQDWIPLEKEISHIND
                     YLVILKIRYGHMLDYKIDIPETLFPVSILKMILQPLIENAVYHGTKYVRHKGFIRITA
                     CKIDEMIQFKVIDNGIGIPAEKLAEIRKELAKGIDSDFSIGYGLYNVNKRLLLYYGND
                     QKPITIDSKYREGTTVTLMIPLSPKTIGGNKHV"
     gene            complement(29885..30901)
                     /locus_tag="IWO89_001108"
     CDS             complement(29885..30901)
                     /locus_tag="IWO89_001108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HAQ0455878.1"
                     /translation="MKRVLFFLALVVIAYQMMGCTPNTKEAPDPLVVGFSQSGTESSW
                     RKKHTESIITALEKEDYQVLYRNGYMNQERQIQDIRTFIAYKVDMIIFTPLQESGWDS
                     VLKEAKRAGIPVILVDRHIQTNDPELFVTHIGPSFKAEGNRAGLFVSNHFTNSKKQEI
                     RILELAGSENSTPTKLRSEGFLESINRKPTLKKDGTIDHKPPMTIVHRIVGDFIRMKG
                     KDQMKRYLETNDLSEIDVLYSHSDEMTHGALAAIKETSIKPGEDLIIVTIDGQEDMIE
                     KLRSGIVNCVVECKPDVGWYVANTVTRYFGNIQTGKTLPKDIFISETVFSQQNIANIP
                     TRNY"
     gene            31121..32209
                     /locus_tag="IWO89_001109"
     CDS             31121..32209
                     /locus_tag="IWO89_001109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HAQ0455879.1"
                     /translation="MKNKFRKIVGTIAILTASILLSACGNSGSADDSTGYVGIAMPTK
                     SAERWIADGNNMVSELEKLGYKTDLQYGEDKVENQVAQIENMITKGVDTLVIASIDGS
                     ALTDVLAKAKEADIKVIAYDRLLMNSENVDYYATFDNFGVGVSQAAYIEEHLGLKEGK
                     GPFTIELFGGSPDDNNALINYNGVMSVLQPYMDNGQLVVPSGQTSFSQIATLRWDGST
                     AQARMDNLLSANYTDQTLDAVLSPYDPISLGIISSLKGVGYGSESKPLPVITGQDATV
                     AGVKSIIAGEQTQTIFKDTRILAKNTIEMIKAISDGEEVPVNDTETYDNGVKTVPTYL
                     ANTVSVDKDNYQAELIDTDYYKESDLKN"
     gene            32263..>33568
                     /locus_tag="IWO89_001110"
     CDS             32263..>33568
                     /locus_tag="IWO89_001110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAQ0455880.1"
                     /translation="MADCILKMADITKKFSGIRALDCVNLMIERGEIHALCGENGAGK
                     STLMNVLSGLYPYGSYDGEIFYDGKLCQFKDLKDSEEKGIVIIHQELALSPYLSIKEN
                     IFLGNEQAERGVIDWELTEKKTYELLNKVGLSIDPNLLVSQIGVGQQQLVEIAKALSK
                     SVRLLILDEPTAALNEEESANLLRLIRELKEQGVTSIIISHKLNEIVDIADRITILRD
                     GQTIETLENEMISEEHIIRGMVGRDLVDRYPERKPDIGEVYFKVEDWTVYHPLDHERI
                     INDHINLHLKKGEIVGIAGLMGAGRTEFAMSLFGHSYGSKISGRILKEGAEISVKTVP
                     SAIANGLAYVSEDRKSLGLNLLMDIRENVTLSSLDKISQKGVLDKEKEVTEAENFRKK
                     MRIKTNSIHQIVSSLSGGNQQKVVLSKWLMTQPDILFLDEPTR"
BASE COUNT        10672 a         5736 c         7518 g         9642 t
ORIGIN      
        1 tttttcacaa ccttttaaaa ttacatattt ttccatagaa cttgcatttg atccaagatt
       61 tcgatgacat cttttgtcaa gaagagagag cgattgggca agtcaccttc aatcatgtgt
      121 accatattct ttatttcgta attcattgct tcagatgtat tcccgctttc gatgaattcc
      181 tttgtaccat ctgtaaataa gatctccgca cgatcagcac gcgggtaatc agcaatcgtc
      241 acataaccgt tctcaaaggc aacgataccg acttttggca tttttgcctg aaaagtcaag
      301 ctgacagcag caagttcatt ttctttgtta cgcaagatag tgacggattg ttcgtccacg
      361 cctgtttcaa aaggaaccat tgtagaggca actacttcag gttgagagct caaaaagaaa
      421 cgagcgaaag atacagcata cgtaccgata tccaacagag ctcctccagc taattctgga
      481 ttgaagaacc ggtttttagg gtctggttct ttgtaactgc cgaaaggtgc ttggatcatt
      541 tttaaagccc ccagttttcc tgtatccatt attgagcgta actgctgata aagtggcatg
      601 ttgaagattg tcatcgcttc tgctaagatc aaattctttt cttctgctaa gcgcatagcg
      661 tcagccagtt cttttttatt catcgtgatg gctttttcac atagaacgtg tttgcctgct
      721 tccaatgctg ccaagatatg gtcgatatgc tgtctatttg gtacagcaat gtacacgatg
      781 tcgatttcag catcgtctaa catttcttga tagcttccgt aagcttttgg aatattgtaa
      841 cggtgagcga agttttccgc tttttctgaa gttctggaag caactgctgt tagttcgctc
      901 gtctcttgat tgaagctttc ggcaaactca tgtgcgatgg taccaagacc aacgattccc
      961 cattgatatt ttttcatctg aaaacgctcc ttcatagttg gattgagttt cttgtctgtc
     1021 tattattata acaaaaatca cgaagaataa agaggagagg acttgaaaca ttaaaaaaaa
     1081 tgaaagcagc gatttaagag gtgctttttg atcaaaactt aagtagaata gttaaaagga
     1141 ggggataaac gtgcatcaag tatatattga tcttattatg agtgtattat tagaacagtt
     1201 tgaaactgaa aaaaattttt tagatgaata cttgctgatc agtccagaac aatggcaaaa
     1261 atggaaagat ggcgagaaaa atctgccatc tgaagcgatg cagaaagtaa aaaatctttt
     1321 cactgattat gaatggatgc taacgcaaaa gattttgcgt caaacgatta tttttcccga
     1381 aaaacgaaac gttgccgttg cagagtacaa gcaaatcaaa acacgcatcg cgcaaaaatg
     1441 gcttagcagc aaagcagcaa aagtcgagct gatccctttg aaaaataatc agcaggctgg
     1501 tagatacctt gacttgaaag taagtattca atatgatgaa tggggctatg atgatatttt
     1561 gaattttcgt ctgccagcag atgttcaaaa acagatcgaa ggagaaaaaa tcgaacttct
     1621 cgattgggtg aatgaaaatc tggaagaaac ttacgttcat gaggcgaatg gggaacgtga
     1681 agaacaacga aaggattttt caagtgaata aatttttagg atttggagca gttgtgatta
     1741 tgatcgcagc aatgggctat gttattttta gcgatgatga ttcgcatgtt caagccggca
     1801 atcaagcagt cgaacgaaca agcaccaaaa caactgctag taaagaaaag aacagtcaaa
     1861 caaccagcag caaggtaaag gacaaagaaa caaaagatcg tacgtccaaa caattgccag
     1921 atgttaaaac agacgattgg aagctagttt tggttggtcc agatcacaaa ttagaaaaag
     1981 aaattgatga gtctactcaa ttggcatcat taagtaacgg atacttgatc gacaaacgca
     2041 ttgctccaga atatgaagca tttgccaagg ctgctgaagc agctggattt ccattagtga
     2101 tggtctcagc ttaccggtct gtatcatccc aacaacaagt tttcgaacaa aatgtccaag
     2161 atgtgatgag tcgtcaaggt gtatcagaag aggaggcgac aaagattact aaagaaacga
     2221 ttacggagcc aggatacagc gaacaccaca caggtctggc agtcgatgtc gtcgatcaaa
     2281 actggtacaa cagttatcct tctactgtat tggatgcaag ttacggagat cagcctggag
     2341 ctaaatggat cgcagataat gctgcgcagt atggatttat cgttcgttat ccaaaaggac
     2401 acgaagatat aactaagatc acatatgaac cttggcactt acgttatgtc ggtaaagaaa
     2461 acgctgagta tatcaccaaa caccaattga cgctagaaga atatctgaat gaattaaatg
     2521 aaaagtagga attgatcatg gcgaaaaaaa agtggaaaaa gcaaaagcgc aagaatatcc
     2581 gttggccagc agtggttgcc ggtgtagcag tgatactctt agcctttgtt ttttccttga
     2641 agaatctgtc ttctcctttt actgataatc agcaagaaaa tgaagcgcaa tcccatcagc
     2701 aatttattga acggcttgtt ccgcatgccc aagaactaca agatggttat ggagtgcttc
     2761 ctagtatcat tttaggacaa gctatattag aatcaaattg gggaaaaagt accttggcta
     2821 gcaaatacaa taatcttttc gggatcaagg cttatgggga tcagaagaaa gtaagcctgg
     2881 aaacaaagga atttgttaac gaagaatgga taactatcca aggagacttc aaagtgtatg
     2941 attcatggga acagtccatg gatgaccata cacaactgtt cgtccaagga gtagattgga
     3001 atcctgcgct gtatgaaaaa gtgattacgg ccaccaacta tcaggaagcc gcacaagcat
     3061 tgcaagatgc aggttatgcg acagaccccg gttatgcgca aaaaatcatc caggtcatcg
     3121 agacgtatca attgaatcag tatgaccgga gatagcaaag gagaagtggc tgatggcaga
     3181 acaatttacg ccagaaatca tgacggaatt gagaaaagat atcgtaagag taccggaaat
     3241 cattttagaa gcaagcggga tcaagatatt tggaaaattg atccgttcga tcatctttac
     3301 tacggatatt gcgatcatac gtaatacaaa tgcgaatgca gtaattgctg tgtatccttt
     3361 tacacctcat ccagcgatta ctaaaagcat tattgaggca gctgacattc cagttttctc
     3421 cggtgtaggt ggtggtttga cacaaggtgc tcgttcttcc tacatgagca tgttcgcaga
     3481 agcgcaaggt tcgttagggg tcgttttgaa cggtcctacg cctttagaga cagtaaaaga
     3541 agtttgtcga gtagttgata ttccagttat cagtacggta acgtcgaaat atacagaaat
     3601 agatgaaaag ttagcagctg gtatttcgat tatcaatatc agtgcaggta aagaaactgc
     3661 agaagcagta cgccattttc gtaaaaaata tcctgaactt cctatcattg cgacaggtgg
     3721 tccaacggaa gaaagtattg ctgagaccat tcaagcagga gcaaatgcca tcacatatac
     3781 gccaccaagt agcggagagc tttttagtcg taaaatggaa aaatatcggg aaactgagaa
     3841 aaagaatcat gaataacagg tgagaatcaa aagacgagga agagcagata gtggctcttc
     3901 ctcgtctttt gtactccagt actatttgtt cagaattcaa aaaaaagaga aatttttttg
     3961 tttttttacc tgtaatgtac ataagaagag ttaaaacctt ttgttattat tagcattcta
     4021 acaaaaaaat gtcttgattt attgaagcgc tttctataga atgaaaaacg aataaaaact
     4081 aaaaaaggca cccgacaatt tagtagcagc taaattgtcg gggcttgaaa agtcagcggg
     4141 aactgaccaa tcaagttgcc atagtcaatg cttgtgcatc gacgtgtata ttgtacctaa
     4201 tttcatacag aattactagt tatttttctg ttttttaggg aagagcgcac acgaagaagc
     4261 atgcttattt agctatgtca aggatattgg gcagttgtct gatatccttt tttctttgcg
     4321 taattatcct cccatcattg aagcaagaag tgtttttagc gatgaaagaa gaatcagcac
     4381 tatctaattt ttcttgaaag tattgagttg ttcatcctag gcagaacgat aaggcaagtc
     4441 ttggttttta ttccccttga aaaagatcgt ttaataaata tgttctgtat aaaaatacag
     4501 tatattgaaa aataacaatg gaagtagagg tgcagagaat gaagaaaaga atgagaatca
     4561 tcttagcttt aagtgttatg ttattcttct tgacggcttg tgggcaagaa gatgacaaaa
     4621 aaatcacgct gaacatggct tggtggggtt cgcaagtccg acacgatgca acagtaaaag
     4681 taatcgagct ttatgaagaa aaaaatcccc atgtgacgat cgattatgaa ttttatgatt
     4741 atgaaggata ttacacaaaa ttaggtacac tagcagcatc agatgatatc tgggacattt
     4801 tccaactgaa caatcaattt cctcaataca tcaatcagat tgaaccatta gatgaatatg
     4861 tggagcaagg gataattgat atttctaatg cagatgaatc ctatttagca acgacgactt
     4921 acgacgggca attagttgga ctgtctaacg gggtgaattc gtttgcgatt gcttacaata
     4981 aaaatatatt tgaacaatta aatatccctg aaccagcttc aaattggaca tgggatgaat
     5041 ttgaacaaat cagctatcaa atcactgaag aattaggaat ctatgctatt tcaagattcg
     5101 aagattttat agctggctgt attattcaaa taccacaagt tgagaaagga ttgaattttt
     5161 ataatcggga agatctatct aacagcttgg gatttgaaaa tccagactat ctaacagatt
     5221 ttttccagct gcgtaaaaat ctggtagaca aaggggccca tccagaacca ggtggtggag
     5281 ctgcaagtac gacggatctg tcttttcagg cggttaggga tagagaagcg gcaatggtgt
     5341 ttctatcaag taaccagttg acagaaatat tgtcaggggc tcctgaagat atggagattc
     5401 agttgattaa tccacctcgc agaaaagcag atggagaatc tggtatccct ttacgttctt
     5461 cgcaaatgct gagtatggca aaaagctcag cccataaaga agaagctgct aagtttattg
     5521 atttctttca aaacagtgaa gaagccaatg aaattttaaa aggagaacgt ggtgtaccaa
     5581 tcatgtcgaa tattcgagaa cagctcgctc gtgcagatga tgcaactttg caagcgacat
     5641 ttgattattt agaaatgatt tccgatatgg acaacggcga agtgaatgtc tttgatagtg
     5701 ctttgaatcc ggaaattcaa gaccaataca atcttttagt tgaaaaagtc atttatgaag
     5761 aagtaacagc agaaggaagg catgaaaaaa gaaaaactaa aacggttctt ggatcaagac
     5821 aatgtggtcg gttacgtatt tgcttctcca ttcatcctag gatttctatt gataacagtg
     5881 ataccgatga tcttgtcact ttattattcg ttcaccgatt atcgtttggg aaatccagtc
     5941 tcatggatcg gcttggataa ttatataaag atgctgagtg atgcccgttt tttgaactcc
     6001 atccgagtca cttttctcta tgtatttgca tctgtacctg tgaaactgat ctttggtcta
     6061 ttgattgctt atcttttaac gaggaatatc aaaggaacga ctttctttcg ttctttgtat
     6121 tatgtgccta gtttgatcgg tggcagtatt tctatcgctc tagtttggcg ggagttgttt
     6181 tctaaaaacg ggttagtgaa tattttcctg gaacgattag gcgcacctgc gctttcctgg
     6241 tttagcgatc aaaaactggt catgatccct cttgttttga tgtctgcttg gcaattcggt
     6301 tcggcaatga tcatctttgc tgcaggtatc aaagagatcc caggctcgta ttatgaagcg
     6361 gcagagatcg atggagcgaa caaatggcag acgttttaca agatcacgtt acctcttctt
     6421 tcgccgatca ttttgtataa cttggttatg cagacaatcg cggcgtttat gacttttacg
     6481 caggcattta tcattaccca aggtggtccg aatgatgcca cgaactttta tgctctttat
     6541 gtgtataacc aagctttttc ttttaattct atggggtatg ctagtgctat ttcgtggatc
     6601 atgctgttga ttatgggagc tgtcaccttt ctgattttca aatcatcaaa aaaatgggtc
     6661 gttgtgtgaa tgagaggagg aagaaatgaa taatacagta acgaaatggt ttcaatacgg
     6721 ttttattttg ctattaggtc tagtcatgat ttatccggtc ttatggatga tcgcaggttc
     6781 ttttaaaacg gatcaagaga ttatgagtgg ttcgctgaat ttgattccag agacttttca
     6841 atgggaaaat tacgcaaaag gatgggcggg gtttgctgga atttcatttt ttactttctt
     6901 taggaattcg tttgtcatct ctgcagtttc gacgataggg acagttctga gctcgacttg
     6961 tattgcttac gcattatcga gaatcgattt caaaggaaaa agattctggt tcgtcgtcat
     7021 gttagtaacg atgatgattc ctgcacaagt gattttgatt ccgcagttta ttatttacaa
     7081 tcggctgaat ttggttggga cgtatgtgcc gctgatttta ccacattttt ttggacaagc
     7141 atttttcatt taccagatca tgcaattcat ggtcagcatc cccaaagagt tagatgaatc
     7201 agcgattatt gacggatgca gcaagtatag tgtctttacc aaaattattt ttcctttgct
     7261 gaaaccttcg atcatcacga cgatcatcat ccagttttat tggaaatggg acgacttcat
     7321 gggaccattg atttatttaa acaagccgag atcttacacg gtatcgattg ccatcaaatt
     7381 atttgcagac gctggatcta gtacgactta cgcgagtatg tttgcgatgt ctacgttgtc
     7441 gctgctaccg gtttttctga ttttcttatt cttcaataaa tatcttgtcc aggggatcag
     7501 tacaagcggt ctgaagggct gaatatgaaa aagccaatag aagagatgtt tctttacaag
     7561 ctcaactact atgtctgtcg gattttttta accagtctta tcagtaatgc tcttttcgct
     7621 ttagttctag gatgtttgtt cactttagga ttagtgctgt ctgctcgtgt tgtctatctg
     7681 atttattttt tgatgatgta tccttcttat tatctatctg tccattactt ggcagaaaaa
     7741 aaggtatgtt ggaggaaagg gaaaatcaaa cggatgtgtt tcatcattac gttgtttcag
     7801 ctgtttgtca ttatttttgc agttgattgg tatttattag tgggaaagat tggagcttgg
     7861 tatctgattc cttttctatt gtgtctgttg ctttttgtat cccatagttt ttttagtacg
     7921 ctaattgata tctgcaaaga agataaagca gatagtcttg tttatttgat caaaaggaat
     7981 atctggctgt tgtcaaaaaa cgtcaagggg tttctgtggt atttatgcct tttgtatgga
     8041 ctgttctggt gtatacagcc tgatttttta ctcactgcat tactgtatcc agggctgcca
     8101 tttgtcacag tattttgctt aaaagaaaag gagaatgact ttaaatgaag aaaaaatatg
     8161 tacaagtcgg acttggtggg agagcgcgtt ttttttatga ggcaattgcc tctacgtatc
     8221 atcaaacttg tgaactagtc ggtttttgtg atgtatctca aacaaggatg gattatgcaa
     8281 atcggatact tgtgaacaag tatggacaga aaaaagtaaa gacatgtatg ccatcacaat
     8341 ttgagcaact actggatgac gtacggccgg ctgaagtcat tattacgtca gtagatcgaa
     8401 cgcatcatga ttatatcatt cgtgcaatga aaaaaggata tgatgttatt tgcgaaaaac
     8461 ctctgactat cgatgaggaa aaagctcaag aaataataac ggtccaaaaa cagacgggaa
     8521 gaagcctgcg agtcactttt aattatcgtt atgcaccgta tcacacgaag ataaaggaac
     8581 tgatagaaag tgatgtgatt ggtgatgtgt tttctatcca cttcgaatgg ctgttaaata
     8641 cgcaacacgg ggcagattat ttcagaagat ggcatcgaaa taagcacaat agcggcgggc
     8701 ttcttgttca taaatctacc catcattttg atctagtgaa tttttggttg aacagccgtc
     8761 cccaaaaggt atttgcattc ggtggattaa acttttatgg gatgcataat gcggagaaac
     8821 gcggagtaga aatattttac agtcgatgct atggaagcga actagctaaa gctgatccat
     8881 ttgctataga cctgtcagcc aatgaggaat tgcgagaaat gtatttagaa gcagaaaaag
     8941 acagtggata tattcgtgac cgcagtgtat tttccgatga tatctctatt gaagatacac
     9001 taggcgtaac ggtcaaatat gaaactggtg cgattttgac gtattcgcta aacgcgtata
     9061 tgccgtggga aggattcaat gtttctttca atggcagcaa aggacggatc gaaatgaaag
     9121 tgatcgaaaa gtcttacatc aatgcaggtg gagataaaaa ggatgaagga gcagccgttc
     9181 ttcagtccat ccatgtctat ccgatgttcg gtgagccata tgaggtaatc gtcgaacaag
     9241 aagcgggcgg acatggcgga ggagatccgc gtttattaga tgatctattt ggtgaagaaa
     9301 aagaagaaga tccgtggaat cgggcagcaa ctcacgtaga tggtattttg tcgattttga
     9361 caggaatcgc agcaaatcat tctattgctt caggaaaagc agtagtgatt gacgagcttg
     9421 tcagcttaaa agaataagaa tagatttacg gaaattactt cccgtagttt ataaaatctt
     9481 gccttattgc cgataggctg tcttttgaac actatttatt cggaaaaagg ggctatgaca
     9541 tgctttttgt catgccttct ttttatgtgg gctgcttttt tagataggag aaaataaaaa
     9601 taactattta aacccgaaca aaaaggaata atgcattaaa aatcattaga ttttacaaaa
     9661 aaataagtaa accacgttgt cagtttcaga aagtagtgtt agactgtttt caatatttga
     9721 aacaacattt tttattcaaa tatggcaaac tgccttcacg tgcaaaacag ttgcaagaaa
     9781 atgaatttac actattatta gttttacata cctacttata tatcgtcata atatggatga
     9841 cagtttctag ccagtaccgt aaatgctgga ctataagtaa aagattggct atttcaccct
     9901 tcttttctta tgataaagtg gagggtgttt ttctttaaca gaaacatagt aggtttttgc
     9961 acaaaagcgg ttatttcggt attaataaat gataaaggag caaataaagt gaaagaatta
    10021 gttgaacgta ttcaaaaaga cggacgtata ttaggcgagg gcgtattaaa agtcgacagc
    10081 ttcgtcactc accaagtaga ccctgtattg atggaacaaa tgggaaaacg gtttgcagaa
    10141 gtatttaaag aacaaggaat cacgaaagtt gtgaccattg aagcttctgg gatcgcacca
    10201 gctttatttg ctgcaagaga attagatgtt ccaatgatct tcgctagaaa agcaaaaagt
    10261 ttgacgatgg atgaagaatt attgacagct tctgtttatt cttttacgaa acaagtgaca
    10321 agtacgattt ctatttctcg taaattttta tcttcagaag acaaagtatt gattatcgac
    10381 gatttcttgg caaacggaca agcggcaaaa ggattgatcg aattatgcca gcaagctgga
    10441 gcgcaagtag aaggaatcgg gatcgtcatc gaaaagtctt tccaagatgg acgtcagtta
    10501 cttgaagaaa tgggattgag agtcgtttct cttgcgcgta ttgcttcctt gacgaatgga
    10561 caagttgagt ttcttgagga ggacgcataa tcgtggaaaa agaaacacaa aatggaaaag
    10621 ctgcggtttt agggctgcag catctgctag cgatgtatgc aggagcagta gcggtgcctc
    10681 ttttgatcgg tacaggacta ggctttaatc aagaacagat gacttatttg atttcaattg
    10741 atatctttat gtgtggatta gcgacattat tgcaattgac agttaaccga tttttcggaa
    10801 ttggcttgcc tgtcgtttta ggatgtgcga tccaagcagt agctccactg atcttgatcg
    10861 gaaccaatga aggagtagga gctatctacg gatctattat cgcttctggg atctttgttg
    10921 tgttagtttc tggtgtcttt tctaaaatta aaaaactatt ccctcctata gtaacaggaa
    10981 cggttatcac cgttattggt ttgactttga ttcctgtagc aatcgagaaa atgggtggag
    11041 gtaatgcaac agcagctgat tttggtgaca aaacaaatct gctacttgca tttgtgacga
    11101 ttctcctaat tgtaggcgtg caaatgctgg caaaagggtt cattcgttca atcgcggtat
    11161 tgattggttt agtcggagga agcatcttag ctgctttcat gggaatgatt gatctgagcg
    11221 cgattggaga tgcgccgttg ttccatgttc cacaaccctt ttattttggt aaaccaactt
    11281 tcgacgtatg gtcgatttta ctaatgatca tcatttccat ggtcagcatg gtagaatcaa
    11341 caggtgtata ttttgcactt ggtgacatta ctgggaaaaa tgttggagaa gaagaattga
    11401 aaaagggata tcgtgcagaa ggacttgccg ttatcttagg cggtattttc aatacattcc
    11461 cttatactgg cttttctcaa aatgtcggat tggttcaact ttcgggaatc aaaacaagaa
    11521 aacccatcta tttctctgca ttcttcttga ttgttttagg attattccct aaaatcggtg
    11581 cgttagcaca aatcatccct gagccggtac ttggtggagg aatgttagtc atgttcggga
    11641 tggtggcagt ccaaggaatc cgtatgctgt tggaagttga tttcagcaat gataaaaatc
    11701 tattgattgt tgctgtttct gttggatttg ggctgggttt caatgttacg ccgacattat
    11761 tcagtcaaat gccgcaaaca ttgcaaatgt tcactggaaa tggcatcgta atgagtagca
    11821 ttacggctat catattgaat ttgatcttta atggattgaa aaaagatgaa gaaatattgg
    11881 aagaagcctt caagaagtaa tagagcaaaa gaaaggttgt gaaatcagcg ttttgacctg
    11941 attgttagag aaacaaacgg aaaaatagtt cctcgtattt tgccgtttgt ttgagctaat
    12001 accgagggtc agctgattga acaccgttta tccataaaaa ggaatgtgac ctaacttttg
    12061 tcacattcct ttttttcatc tttaataaac acgaacaata aaatgttttt ctttgttaac
    12121 attcgaaatt tctttgacat caaaaaaagt gattgttaaa atatgaatgt agaaaaactg
    12181 ggaggaaaaa caatgacagc aaaaatctct gtgattatgg ggagtacttc agattgggaa
    12241 acaatgaaag aagcatgtca aatattggat gaattcggtg tgtcttatga aaaaaaggtc
    12301 gtttctgctc atcgaacccc agatttgatg tttgaatatg cacaaaacgc tcgaaaaaaa
    12361 ggaatcaaaa tcattattgc aggagcaggg ggagccgcgc atctgccggg tatggtcgca
    12421 tcaaaaacaa ctttgccggt tatcggtgta ccggtccaat cacgaacatt aaatggtttg
    12481 gattcgcttc tttcgattgt ccaaatgcct ggaggagtac ctgtagctac tacagcaatc
    12541 ggaaaagcgg gagcaattaa tgctggacta cttgcgatac agatgctttc catgtatgat
    12601 ttagaattgg aggctaaatt ggctgaaaga cgttcaatgt tggctaaaac tgttatagaa
    12661 agtagtgatc aacttggtta aacctttgtt gcctggttca acgatcggga tcgtcggtgg
    12721 cggtcaatta ggtcgtatga tggcgattag tgcaaaagaa atgggcttta aagtaggtgt
    12781 gctggatcct gtgagcgatt gtccagccgc acaagttgca gattggcaca ttgaaagtgt
    12841 ctatgatgac acctttgcgc tgaaagagct tgccagaaga acagatgtca ttacctatga
    12901 attcgaaaac gtcagtgtag aggcgttaaa tgcgatttta cccatgtcat ttattcctca
    12961 agggacagac ttgcttgcga ttacgcaaga ccgcctgtta gaaaaatctt ttttggaaac
    13021 gaataacatc gtgattgctc cttatgcaac gatcgtcagt ccgacggata tccaagatgc
    13081 gattgacggg atcggctatc cttgtgtttt gaaaaccact cgaggcggct atgatggcaa
    13141 ggggcagtat gttttgaaaa gtcgggcgga tctagctcct gccatggacc ttttaagaga
    13201 aggaacctgt gttttagaag catggatccc gtttgaaaaa gagatttcga tcatggtggc
    13261 aggaaatggt caaggagatt tcacgacttt tccggtcgtg gaaaacatcc accacaacaa
    13321 tattcttcat gaaacgattg ctccagccgc gattgatcag gatgtgatcg aggaggctga
    13381 gcgtattgcc cgtgtgattg cagaagcagt gagcctttca ggtgttttag gggtagaaat
    13441 gtttttaaca aaaacaggcg ggctatatgt gaatgaatta gctcctcgtc ctcacaattc
    13501 tggacattat tctattgaag cttgttctat gagtcagttc gatgcccata ttcgaggtat
    13561 ttgtggttgg ccattaggag aaaaagtacg tttgataagc aatgcagtaa tggtcaatgt
    13621 tttgggagaa caactgctgg aaagctatcg cttgattgag aataagccag actggcaatt
    13681 ccatttctat ggaaaaaaac aagcaaaaaa aggtcgcaag atgggacata tcactatctt
    13741 gacagaagac atccagcaaa cgctggaaga aatcaaacaa acgaatattt gggattagag
    13801 gagaaaaaac atgttagaac gttatacacg ccctgaaatg gggaatatct ggacggaaga
    13861 gaacaaatac cgtgcgtggc tcgaagtcga gattttagca gatgaagctt gggcagaact
    13921 tggtgaaatc ccgcaagaag atgtgaaaaa aattcgtgag catgcatcat ttgatatcga
    13981 tcggatcttg gaaatcgaaa aattaacacg ccatgatgtc gttgcgttta cacgagcggt
    14041 ttctgaaaca ttgggagaag aaagaaaatg ggtccattat ggtctaacaa gtacggatgt
    14101 cgtagatact gcttatggct atctcatcaa gcaagcaaat gatattttac gggaagactt
    14161 gaaacgtttt acggaaatca ttggcgaaaa agcaaaagaa cacaaatata cagtgatgat
    14221 gggtcggaca cacggtgtcc atgctgaacc aactactttt ggattgaaac ttgccacatg
    14281 gtattcagaa atgaaacgga acatcgaacg atttgaacat gcggcaaaag gagtagaagc
    14341 aggaaaaatc tcaggagcag tgggaacgtt cgcaaatatc ccgccattta ttgaagagta
    14401 tgtatgtaaa caattgggga tccgtccaca agaaatctct actcaagttc tccctcggga
    14461 tctacatgct gaatatttct cagcgatggc gcttattgct acaagtattg aacgttttgc
    14521 aacagagata cgcggattgc aaaaatccga aacacgtgaa gtagaagaat tttttgcgaa
    14581 aggtcaaaaa ggttcttctg ctatgccgca caaaaggaat ccgatcggtt cagaaaatat
    14641 ggctggtcta gccagagtgg tgcgcggaca catggtcact gcctatgaaa atgtcacatt
    14701 atggcatgaa agagatattt ctcattcttc agcagaacga atcatcttgc ctgataccac
    14761 gattttattg aattacatgt taaatcgttt tggcaatatc gttaaaaatc taacggtctt
    14821 tcctgaaaac atgaagcgga atatgtctgc aacatttggt ttgatttata gccagcgcgt
    14881 tatgctgaaa ttgatcgata agggaatgac aagagaagaa gcttatgatc ttgttcagcc
    14941 aaaaactgcg tacgcttggg acaaccaagt gcagttccgc ccgctgttag aagaagatcc
    15001 aaccatcaca tcggtattat ctaaagaaga aatagacgat gcctttgatt atcatcatca
    15061 tttgaaaaac gttgacgtga tattcaaacg agtaggtttg gaataaagaa gagtgagttg
    15121 caaaattcaa ttaaaataga atagcaaaaa taaccaaaaa agaagcagat ccgttagcgg
    15181 ttctgcttct tttagtttat tatattttct tatcaataat aggaacctga ttttaaaagc
    15241 ggctacctct ttaccgtgcc tctttacata caaaaaatag aggaattaag cgtaaatggc
    15301 ttggctatta taactaaaat gctggttatg gagatgttgc agcaagtttt cgagaaaagc
    15361 gtattccatc tcttgtccat tgaataagat ctggatacga taatgatttt tgaaacgctt
    15421 ctgttcatga atctgactga ttgaaagttc gcctttgtgt tctgtctgat aaagaaaagt
    15481 ccttttacgg cattcagcac agcgcttggt tgctcggcgg atttttcgga atgtataaaa
    15541 actagtatct ttattgcata aataaacgtc tttgatcgac agcaaatcat tatatagaca
    15601 ggaacgttct tcagctgtta attctgataa aaaaagctga ccttgtttta ttgcatcttc
    15661 catcggcaag tgcctcctcg aacccattat ccaaattttt agttaattct aatataccat
    15721 cattaagtat aaatgtaaac gcttgttgat ggtataagat tttcgtttct gataaaacac
    15781 gaacattaaa agaatctaat gccttaaatg tacatatttt tgttgattta taaaaagatg
    15841 attgctaaaa tttaaagtga aaaaacgaac aaaaaacgat gtatttgctt ttaatgtaaa
    15901 taaaaaggag agacgtacat ggagaagaaa gaacttttgt atgaaggaaa agccaaaaaa
    15961 atatatctta cttctgatga gaacttgatt tggatagaat atttagatca agcaactgcg
    16021 ttgaatggcg cgaggaaaga ccagataagt ggaaaaggag aattgaataa tcaaatcaca
    16081 agtgcaattt tccgttatct gactcaaaga ggaataaaaa atcattttat tgaacaattg
    16141 tctaaaaccg aacaattagc aaaaaaagta caaattatcc ctctggaagt ggttatccga
    16201 aatattgcag caggaagttt ctcaaaacgt ctaggtgttc ccgaaggtaa agaattcatc
    16261 aaaccaatca ttgaattcta ctataaggat gaccagttgg atgatccatt tatcaatgaa
    16321 gagcatatct atttcttaca acttgcaaaa ccggaagaaa tcagtgagct gaaagcccaa
    16381 gcgttggcta ttaatgaaat tttgaccgaa ttatttgcta aggtcggttt ggttttagta
    16441 gatttcaagc tagaatttgg ccggtcgaat ggacaaattt tgcttgcgga tgaagtaaca
    16501 ccggatactt gccgattatg ggatgaacgt acgaatgaac atatggacaa agacgtttac
    16561 cgcagagatc taggtgattt gatcccagtc tatcaagaag tatataaccg tctatcggaa
    16621 ctaactgaag aaaaggagcg agtatgatgt atttcgtaaa agtatatgta acctataaag
    16681 attcagtgtt agatcctcaa ggggaggcag taaaaaaagc agtccatcgt atgggatatg
    16741 aaacaatcga agatatccga ataggaaaat actttgagat acaacttgct gatgcagaag
    16801 aaccaatcga tacagtgatt gaaacgattt gtgacaagct gcttgcaaat gtaaatatgg
    16861 aaacataccg atatgaaatc aaaaaaatgg aggaagtata acatgaaatt tgcggtcatc
    16921 gtttttccag gttcaaattg tgacgcagat atgttatggg cagttcgcga gatcatgggc
    16981 gctgatgccg aatttgtacg gcatgatgag tcctctttag aaggatttga tggggtttta
    17041 ctgccgggag gattctccta tggtgactat ttgagatgcg gtgcgattgc tcggttttct
    17101 acaatcatgg aagaagtgat tcgttttgct gaagaaggga aaccagtatt cggtacatgc
    17161 aatggattcc agatattgac agaagcagga cttttgccag gtgtactgag acgaaatgag
    17221 tctttgcatt ttatttgcaa aactgtacca ttgaaagtca accccaagac ggcctttact
    17281 tcagagtatg aagccgacga aataatctat cttccagttg ctcatgggga aggaaattat
    17341 ttctgtgacg aagaaacact tcaagaacta aaaaaaaata atcagattgc ctttacttat
    17401 aatgacaaca tcaacggcag cgtggaaaac attgcaggga ttacgaacaa agcagggaat
    17461 gtgttaggaa tgatgcctca tccagaacgt gcagtagaag acttgctagg atcagaagat
    17521 gggaaacggt tctttgcttc cattttaaaa aacttcggaa aggtgaccgt atagatgaaa
    17581 acattgaaag aaccgacagc tgaagagatc aagaaaaaaa gaatctatgc cgaatgggga
    17641 ttgactgatg aagaatatcg aatgatcgaa gaagatatct taggaagaat gcctaattat
    17701 acggaaactg gactgttttc tgttatgtgg agcgaacatt gttcctataa aaattctaaa
    17761 ccggttctac gcaaatttcc tactagtgga cctcatgtgt tgcaaggccc tggagaaggc
    17821 gcaggcatcg tcgacatcgg tgatggacta gcagttgtct tcaaagcaga aagtcacaat
    17881 catccttcag ccgtggagcc ttatgaagga gctgcgacag gagttggcgg aatcatcaga
    17941 gatattttca gcatgggggc tcgtccaatt gcgttgctag attcattgag gttcggcgaa
    18001 ttagataatc caagaacgaa atatttacta gaagaagtag ttgcaggtat cggcggatac
    18061 ggcaattgta tcggcatacc gactgtaggc ggcgaaattg catttgatcc ttgttatgaa
    18121 ggaaacccat tagtcaatgc aatgtgcgta ggtttgattg aacacaaaca tatccagaaa
    18181 gggcaagccg ctggcgtcgg taattcgatc atgtatgtag gtgcaaaaac aggtcgcgac
    18241 ggtatccacg gagcaacttt tgcctcagaa gaatttaatc aagaagaaga acagcaacgt
    18301 tctgctgtac aagtaggcga tccttttatg gaaaagctgc tgcttgaagc gtgtttggaa
    18361 ttaatcttgc atcattcgga ttctctaata ggtatacagg atatgggagc agcaggactt
    18421 gtttcatcta gtgcggaaat ggcttcaaaa gctggctcag gacttatatt gacattagat
    18481 gaagtcccac aaagagaaac tgggatgaca ccttatgaaa tgatgttatc ggaatcacaa
    18541 gaacggatgt tgatttgtgt taaatcagga gaggaagaaa aaatccaaga actatttcag
    18601 aagtatgatc tagatgcagt cactatcggt aaagtaacag acgacgggca atatcggcta
    18661 tatcatcaag gagaagaagt agcaaatctg ccagtcgatg cattagctga agatgcacca
    18721 gtttatcata aagaaatgaa agaaccagct cgaatcgtag aatttcagca gttagctcct
    18781 tatcagccag ttatcgaaga acctggacaa gtattgcttg atctgctgca gcaaccgacg
    18841 attgcttcaa aacgttcaat ctacgaaact tatgattcac aagtacaaac caatacagtt
    18901 gtccgtccag gaagcgatgc tgctgtgatg agggttagag ggacgaataa agcgttagct
    18961 atgaccacag actgcaacgc gcgctatctg tatttggatc caaaaatcgg aggacaaata
    19021 gcagtggcag aagctgctag aaatattatt gctagcggtg gtaagccctt ggcgatcacc
    19081 gactgcctga actatggttc tcctgataaa ccagaagttt tttgggaatt atcgacttca
    19141 gctgacggaa ttgctgctgc gtgtgaaacg ttaggaactc ctgttatttc aggaaatgtc
    19201 tctttatata atgaaacgga tggtcaagca atctatccaa caccaatgat tggtatggtc
    19261 ggattgattg aggatcataa acatatcact actcaggaat tcaaaaaatc aggggatcta
    19321 atctatatat taggtaagac gtttgcagat tttgatggca gtgaattaca aaagatgcag
    19381 cttggaagaa tcgaaggtgt gatccgaaac tttgatctat ccattgaaaa gagaaaccaa
    19441 gagttagtat tgacagccat ccaaaacgga ttgattgaaa gtgctcatga ctgttcagaa
    19501 ggtggcttgg caattgcact ggcagaatca gctttcaaac atcagctagg gatatctgta
    19561 cagtttgaat tgtcctctgc tcaattattt gcagaaactc aatcaagatt tgtgttgaca
    19621 gtcgcaccgg aaaataagac acgattcgaa gaaatgatgg gagatgctgc tgttttagca
    19681 ggtaaggtga cggatgaagc gataatcgaa atttcagcaa ctgacggtca gatcaaaata
    19741 gaaactgcag tagcaagaaa gtgctgggag gatgcaatcg tatgtcttat gaagtgaaga
    19801 gtttaaatga agaatgcggg atcttcggtg tttggggtca tccagacgcc gctagtgtgg
    19861 catacttcgg gctccatagt cttcagcatc gaggacaaga aggtgcaggt atcgtgtcca
    19921 atgatcgagg aaagcttaag gggcatcgag atttggggct gctttctgag gtattcaaag
    19981 atcaaagaaa attgcagcag ttaacaggag aagctgcaat cggacatgta cgttatgcaa
    20041 cagcaggcaa tggaagtgtg gacaatattc agccttttct attcaaattt catgatcagt
    20101 cgatcggctt agcgcataat ggtaacttga tcaatgctcg ttcccttaga aaagagttag
    20161 aggaaaatgg ggcgatcttc cattctaact ccgatacgga gatattgatg catttgatcc
    20221 gacgaagtga agaaacagat tttttaaata aattaaaaag tgctttgaat gaagtcaaag
    20281 gtggctttgc ctatttatta atgacagaaa cggcaatgat tgccgctttg gacccaaatg
    20341 gttttcgtcc attagcaatc ggacaaatga aaaacggagc atacgtgatt gcctcggaaa
    20401 cttgtgcatt agaagttatt ggggcacgtt ttataagaga tgtccagcct ggtgagatag
    20461 tgatcatcaa tgacgaagga atccagattg attgctttac gaaggacacg cagctttcta
    20521 tttgttcgat ggaatatatc tattttgcca gaccagattc aaacatcgca gggatcaatg
    20581 tgcatactgc acggaaaaat atgggacgga atttagcgaa agaatcgcca gtaaaagcag
    20641 atatggtcgt tggcgtacct aattcttctt tgtcagccgc aagcggttat gcggaagcta
    20701 gtggaatccc ttatgaaatc ggattagtaa aaaatcaata cattgcccga acatttatcc
    20761 agccaacgcc tgagctaaga gagcaaggtg ttcgaatgaa attatctgct gtacgaggag
    20821 tagtggaagg taaaaaggtg attatggtcg atgattcaat tgttcggggt acgacaagca
    20881 gacggatcgt ccatcttttg aaagaagcag gggcaaaaga agtccatgtg aggattgctt
    20941 ctcctccttt gaaatatcca tgtttttacg ggattgatat tcaaacgcgt aaagaactca
    21001 ttgcggcaaa tcacacgatt gatgaaatca aagaatgtat cggtgcagat tctttaggtt
    21061 ttttaagtga agagggtctg atcgaatcga tcggtctgga aaaagatgct ccgtattcag
    21121 gattgtgtat ggcttatttc aacggagatt atccgacgcc gttatatgac tatgaagaaa
    21181 aatatcagga atcactaaaa gaaaaagtat cgttctttta aaagcagacc aagaagggag
    21241 tatatgaatt caatggcaaa tgcatacgca aaagctggcg tagatgtcga agctggatat
    21301 gaagtagtag aacggatcaa gaagcatgtc aaaaaaacgg aacgtttagg tatgatgggc
    21361 gctataggag gattcggcgg ttgttttgat ttatcggtct ttcaattaaa agagccagtc
    21421 cttgtctcgg ggaccgatgg cgtggggacg aaattagcag tggcaatcga agcagacaaa
    21481 cacgatacaa tcgggatcga ctgcgtagcg atgtgtgtca atgatatctt agcacaaggc
    21541 gcagagccgt tatatttctt agactactta gcaacgggga aaaatgatcc gttacgtttg
    21601 gaaaatgtag taaaaggtgt agcagatgga tgtgttcaag gaaatatggc gctgattggc
    21661 ggagaaacag ctgaaatgcc tggtatgtac tcaggtaatg attatgatct agccggattt
    21721 gctgtaggga tagctgaaaa atctcaatta ttgacaggag aaacgatcaa cccaggagat
    21781 gtgttgcttg gaatagcatc tagcgggatt cattcaaatg ggtactctct tgttcgcaaa
    21841 attttctttg agcagcagca gttttcttta tcatcaaaac tgccagaatt gaaaaaaaca
    21901 cttggcgaag aattgcttga gccaacgaaa atctatgttt cttccttgct tccgctgatc
    21961 aaagaaaaga aaataaaagg agctgcacat atcactggtg gagggtttgt agaaaatatc
    22021 ccgcgaatgt taccagaatc tatggctgca caaatcaaac ttggctcatg gccgattctt
    22081 ccaatattcg gctgtttaca aaaatatggt gaaattcctg agatggagat gtatgaaatc
    22141 ttcaatatgg gcatcggtat ggtcctggca gtcgaagaat cgaaagtaaa agaaatagta
    22201 cacgaaattg agcaagctgg cgaaaaagtt tatgaaatcg ggaaagtgat cgccaaagaa
    22261 aaacatcgtg tggtgtttat aggagaagaa cgatgagaat cgctgttttt gcttctggaa
    22321 acggaagcaa ctttcaagcg ctagccgatt atctatcgaa aaaaggattg gaatcttcga
    22381 ttgactggtt gttttgtgat cagcctgaag catatgtttt gaaacgtgca actgcgttga
    22441 gtgtaccggc agattgtttt tcacctaaag aatttgattc aaaaaaagag tatgaagaag
    22501 caattttgca taaattgaaa gaaaaaaaga tcgatttgat cgtattagct ggatatatgc
    22561 gaatcattgg accggtattg ctgaaaaatt atgataaaag aatcatcaat atccatccgt
    22621 ctcttttacc tgccttccct ggtttgcatg gcatacgtga tgcctttgaa gctggagtaa
    22681 aagaaacggg tgtcacaatt cattatatcg accaaggtgt agatactgga ccaatcatcc
    22741 ggcaggaaaa agtgagaatt gaacaggagg acacgttcga ttctttagaa gaaaaaatcc
    22801 atagagtaga gcatcgaatc tatccagaag tgattagtga aattattgaa aatggaggct
    22861 atctgaaatg acaagagcat tgatcagtgt atcagataaa actgggattg tagcattcgc
    22921 ccaaggtttg attgaacaag gagtagaaat catttcaaca ggaggaacaa agcatgcgtt
    22981 ggataaagct gggatcaaaa cgctttcgat cgaagaagtg acagggtttc ctgaaatgat
    23041 ggatggccgt gtcaaaacac ttcatcctaa aatccatggt ggtcttttag gaagacgcga
    23101 tctccctagc catatggagg caatggaaac ggaaaatatc caaccaatcg actttgtttg
    23161 tgtcaattta tatcctttca aagaaacgat tatgaaacca gatgtcgaaa tagcagaagc
    23221 tattgaaaac attgatatcg gtggtcctag tatgctccgt agtgcagcaa aaaaccatga
    23281 atttgtcacc tctgttgttg atccatctga ctatgaagaa gtactttcag aattacgtca
    23341 gtatgggcat acttcacttt ctttcagaca aaaactagcg gcaaaagtct ttcgtcatac
    23401 tgcggcatat gatgcactga tcgctcaata cttaacggat tgggtggaag aaaaagaacc
    23461 ggaaaaattg acacttacgt atgaacgaaa acaaacgctt cgttacggtg aaaatagcca
    23521 tcaagccgct gccttttatc agtccgctct tctggaaaaa tattcgatcg cagcagcaaa
    23581 acaactccat gggaaagaac tttcttataa taatatccga gatgcagatg ctgcgctacg
    23641 gatcatgaag gaatatgacg agccaacggt agtcgccttg aaacacatga atccttgtgg
    23701 tattgggaca gcatctacga ttttagaagc gtgggaagca gcgtatgaag cagatcctgt
    23761 ttcaatcttt ggtgggatca tcgttttgaa tcgagaagta gatctgccaa cagcagaaca
    23821 gatgcataag ctgtttctag aaatcatcat tgccccttct tatacacaag aagctttgga
    23881 attactgcaa aaaaagaaaa acatccgatt actgacagta gactttgcgc aggaagaaaa
    23941 tcaaaatcga gaagaaacgg tctcagtctt aggtggactt cttgtacaag atcaagatct
    24001 ggcaatagaa aatgaagaag agtggaaagt cgtgacaaaa cgtaagccga ctccagcaga
    24061 agcaaaagca ttagcgtttg cttggagagc tgtcaaacat gtgaaatcta atgcgatcgt
    24121 actggcaaac gaacgtcaga cagtaggaat cggagccggg cagatgaatc gagtcggttc
    24181 agtaaaaatt gcgattgaac aagcgcaaga gaaaatggaa ggtgcagttt tagctagcga
    24241 tgcttatttt ccaatggatg attcggttga atatgcagcc aaacatggta taaaagcaat
    24301 cattcagcca ggaggaagca tcaaagacca agattctatt gatatggcga ataaatacga
    24361 tctagcaatg gtctttactg atgttcggca ttttagacat tagagaggag tcggataaat
    24421 gaacattttg gtgatcggtg caggtggtcg tgaacatgca ataagtaaaa aacttttagc
    24481 cagtccgaag gtggatacgg tgtattgtgc gccaggaaat ccggggatga caaaagatgg
    24541 tatcaattgt gtcgctatca gtgagcgaga tcattcagcg ttaatccatt ttgttaagaa
    24601 agaggctatt gattggagta tcgttggtcc tgaagttcct ttgttaaatg ggatcgtcga
    24661 tgattttatg gcggaaggat tgaaaatctt cggtccgaca aaagcagcag ctctgatcga
    24721 aggttcgaaa agttttgcta aacaattaat ggatacttat caaatcccga cagctaaatc
    24781 cagaagtttt gaatgttacg aaaaagcagc agcgtatatc aaagaaaatg gtgcgccaat
    24841 cgtagtgaaa gcggacggct tagcagctgg gaaaggtgta gtcgtggcta ccacagtgaa
    24901 ggaagcgctg gatgcggcag aacaaatgtt gaaccagcat cgatttgggg atagtagcaa
    24961 aaaaatcgtc gtagaagagt ttttggcagg agaagaattc tcccttttgg cgtttgtccg
    25021 taataccaag gtgtatccga tggtcatttc gcaagatcat aagcgggcat ttgataacga
    25081 taaagggcca aatacaggcg gaatgggtgc ttattcacca gtaccgcaaa tttcaaaaaa
    25141 gatgattgaa aaagctgtgg aagaagtttt aaaaccagcg gctttgggaa tgaaagaact
    25201 aggacgctct ttcacgggtg ttttatatgc gggtttgatt gctacgaagg aaggaccaaa
    25261 agtgatcgaa ttcaatgcgc gtttcggtga tcctgaaacg caagtcgttc tttctcggtt
    25321 gaagagtgac tttgcgcaag taatcgatga tttattagag aatcgtacag tagagttaaa
    25381 gtggcagcaa gaaggataca atgtaggcgt tgtggttgca gctgccggtt accccgaaga
    25441 atatgagaca ggaatggttc taccagattt tctggaagaa gagctttctg tttactatgc
    25501 tggagtttcc tcaaataacg gcaaactagt tggagcagga ggacgtcttt atttagtaga
    25561 agcatacggt gagacgatca aagaagcaca gaaaaaaatt tacaccgcgt taatggataa
    25621 aacgacagat ggaacatttt atcgtacaga tattggagaa aaagctgcca atagcgaact
    25681 gatcgtgaag taaatcagct gcttccattt ataaaaaata caacaaacat aatcggaagc
    25741 gccgatttta gtgaagcctt ttcataaaga gtgtggtaca cttttgttgt gtatataaat
    25801 aaggaggagt tcagatgaaa aaaattcaat taggtacttc tgatcagaaa gcagctgcag
    25861 tgatcttagg ctgtatgcgt atcaacaatg cggaagatcc agtaaaagtg attgagacag
    25921 cttatgaaaa cggcattgat ttttttgacc atgcagatat ttatggaaac ggtgaatgtg
    25981 aagaaatctt tgcagatgct ctagcaaaaa catcaatcaa acgagaagat ctttttattc
    26041 aaacaaaatg tggaatcgtt ccaggtgtga tgtttgattt ttctaaaaaa catatcattg
    26101 aatctgtaga aaaaagtttg acccgtctga aaatggatta tgtggatgct ttacttcttc
    26161 atcgtccaga tacattggta gaaccagagg aagtagctga agcgtttgat gaattggaac
    26221 ggtcaggaaa agttcgttat tttggcgtca gcaatcaaaa accaatgcaa atcgaattgc
    26281 tgaaaaaata tgttcgtcaa ccattagtag ctaatcagct gcaatttggc ttgaaacata
    26341 ctggaatgat cgatcaaggg attcatgtca atatgacaga tgaagcaagt atcgatcatg
    26401 acggtagtgt acttgattat tcccgattgc atgatatgac gatccaagca tggtcaccat
    26461 accagtatgg attttttgaa ggtgtattta tcgggaacga aaaattccct gaattaaacc
    26521 aaacattaga agaactagca gctaaatatc aaacgacacc aactggcttg gcttctgctt
    26581 ggattttacg ccatccagcc aatatgcaag tgatcgccgg tacgatgaac gtagggcgaa
    26641 tcaaggaaat cgctcaagct tcagaaatta atatgtctcg ggaagattgg tacgaattgt
    26701 accgtgctgc tggaaacatt ttaccttaaa gattgtcata agcaggtcaa aagggctata
    26761 aattaaaaag acagaataac ttgaaagctg ttttcagctt cgagatattc tgtctttttt
    26821 tattgcaaat ggaaagagta tcattgtacg gatttcgttt attagttatt tttgttttta
    26881 aggaaaagct gtgtccgata ttcttttgga gaaatccctt gttttttctt aaataaataa
    26941 ctgaaatagt tagggtcatt ataaccaatt tcaaaagcaa tatctgaaat acgatagtct
    27001 gattgctgca ataatttttt tgctaattcg attctttgtt ctgtcagata atcgatgaat
    27061 gtttggttaa acgattgaga aaaaatcgta ctgaaatgag agggacttaa tgcaacttgt
    27121 tgagcgaccc aagttaagga catatctgga tcggtaaaat tttctttgat aaaatccaat
    27181 gcatggttca aaattggctg atatttgttc cttgaaggct gtgagggcag agcagttaag
    27241 aaagaaatga acatataaac tgtcgaacgg taaagcgagt cgtcggcgct gattgctaag
    27301 cgttgactgg tggatgtgca atgaaccatt tcttgcggga ttttcttttc tttcaggcag
    27361 acatgctgga gtagcttagt caagacaaat aaacgatata atcgtgttcg agttggtgtg
    27421 ccttgtggtt tcgtcaagtg ttcgatgtac ttagatactt cgtttttcga taatgaagac
    27481 aaatcaagtg agaggtcaaa gtaaaagaca gaatcaactg gaagtttcgc ttcttgaatc
    27541 aaatcctcat agctgataat ttgttcttta agggaactgg cggtttcaag tatcatctgc
    27601 gtgatctcaa aagatgcagg taattcgctg attctttctg cgacaggacc aatggcgaca
    27661 gaaaaattat ttttttgtcc cttttctaat tcatggacca acgtttgggc tacctgataa
    27721 ctggtctcga tcacattttc ttcaagagaa tcaactatta aaaatttgat gaatcgagag
    27781 gaaacagcag agaagatcac ttgttgattc tccctaaata aaaacttcaa ataatcactg
    27841 agatgagcgt aatcttcaaa gtctgtactg acttggtttg ctgctaataa tacggtcatt
    27901 tttttccctg ctaaagagcg attgaaagat tcagcaagtt tcaatgtttc actgatcgta
    27961 agtgagcctt tgaacaaacc gttcaagaaa tgattttttt tcaattcgaa aacaagatca
    28021 gaagaagtgt gcgtttcttt ttgctgcaaa tctaaagcag ttactgcttt ttctattgct
    28081 tgtttcaatt cgctactttt aatcggttta agcagataat catctgcctg tacctggatt
    28141 gctcctttca tataatcgaa atcgtcgaat ccactaataa atatgagacg agtccaagga
    28201 agtagtttcc ttgcttcttt tgaaaaagtc aacccgtcca tgaaaggcat ttggatatca
    28261 gtcagaatga tgtcgggctg taagtcgata attgaagaca gtgccatctc accatctcca
    28321 gcttcgccga cataagtgat tggataataa gaaccaagga cagttatttg ttcccgcaag
    28381 ttattgcgaa tgaggtattc gtcttcgatg ataaaaactt tatacatgtt tgtttcctcc
    28441 aattgttttg ggagatagag ggatcatcag tgtgacagtt gttccttctc ggtatttgct
    28501 gtcaatcgtg ataggttttt gatcatttcc gtaataaaga aggagacgct tgtttacgtt
    28561 gtaaagtccg tagccgatac tgaaatctga atcgatccct ttggctaact ctttacgtat
    28621 ttctgccagt ttttctgcag ggatgccaat cccgttgtct ataactttga attggatcat
    28681 ctcatcaatt ttgcatgcgg taatacgaat aaatcctttg tgacggacgt acttcgttcc
    28741 atggtataca gcattttcaa tcaatggctg caagatcatt tttaaaatag aaacaggaaa
    28801 aagtgtttct ggtatatcga tcttatagtc aagcatatgg ccgtatctta tttttaagat
    28861 gactagataa tcattgatat ggctaatttc tttttcaaga ggaatccagt cttgtccttt
    28921 gcttaaagac gtacgaaaaa agccggaaag tgcatgggtc atagtcagag cttcttcaaa
    28981 gcagtcttgt tcgatcaaag aaataatggc atccaaactg ttataaataa aatggggagt
    29041 aatctgagct tgcaatacac gaacttcact ttgtgctaaa tggtattgtt tcaaagcgtt
    29101 ctcccgtaaa gactggttta gatcttccgc cattttattc atgctagcgg taagtgtttg
    29161 taattccagt gtttccggca gggttaaacg aaagccaagg ttccctgaag aaagttgttc
    29221 tgacatctcg atcagctgat tgatcggttc ttggaccttt ctctctaaaa agcgacgatt
    29281 ctttttcgca tagaaaaaaa tggtactaag aactccaagt tccactattg ttaatagaat
    29341 gagcgagcgg acaagtgtcg tatttttttt gcttgctaag tcgatctcca cacgaacaaa
    29401 atcttgtaag atatctgaca gcagttgagt gacagaatct acctgattga tgatctcttc
    29461 attggtatcc ataaaatctt ttgccaagat attattgatc agctcgtttt gataatcttc
    29521 cagtgtatcg atcgtacgta gcgatacatc taacgtacta atttctttaa gtgtatttgt
    29581 attatttttg atctctcgaa tatcattcct aagttcttct aggatatttt cctcattgaa
    29641 ctcatctacg gttacttggc cagaaatcaa gtcccacata ctttccaaaa ctttttgatc
    29701 aaccttcaaa gcaattgcat ctgctttttg gatatttgcg atcgtctgtt gataaatcaa
    29761 aagctgttgc gaatataaca gtatgctcag tagcaaaggg ataacagcaa atagaagcat
    29821 ggctttgttt gcttgggcaa aaatctgttt tatgcttaaa ttttttgtca tgatacttct
    29881 ccagtcaata atttctggtt gggatgttag ctatattctg ctgggaaaag acagtctcgg
    29941 aaataaaaat atctttaggt aatgtttttc ctgtttgaat atttccaaaa taacgagtaa
    30001 cggtattggc tacataccag ccgacatcag gtttacactc gactacacaa ttgacgattc
    30061 ctgagcgtag cttctcgatc atatcttctt gtccatcaat agttacaatg atcaaatctt
    30121 ctccaggttt gattgatgtt tcttttatcg cagctaaagc accgtgagtc atttcatcac
    30181 tatggctgta taatacatcg atttcggata aatcatttgt ttctaaatat cgtttcatct
    30241 ggtcttttcc tttcatccga ataaaatcac cgacaattcg gtgaacaata gtcatcggag
    30301 gtttatggtc gatggttcca tcttttttta gggttggttt acgattgatt gattccaaaa
    30361 atccttcgga gcgtagcttg gtcggtgtgg agttctctga accagcaagt tctaggattc
    30421 tgatttcctg tttctttgaa tttgtgaagt gattgctgac aaataaaccc gcccgatttc
    30481 cttctgcttt gaaactgggg ccaatgtggg tgacgaaaag ctcgggatca ttcgtttgaa
    30541 tatgtctgtc gacaagtatg acagggatcc ctgctcgctt tgcttctttt aatacagagt
    30601 cccacccgga ttcttgcaaa ggagtaaaaa tgatcatatc cactttatag gcaatgaacg
    30661 tccgtatatc ttggatttgc cgttcctgat tcatgtagcc atttcggtag agaacttggt
    30721 aatcttcttt ttcaagcgca gtgataattg attcagtatg ctttttccgc cagctactct
    30781 ccgttcctga ttgcgaaaaa ccaacgacta gtgggtcagg tgcttccttt gtattaggtg
    30841 tacaccccat catttggtaa gcgattacaa caagggctaa aaaaaataaa acacgtttca
    30901 ctttaaatca ctccatctgg atttgttagt actatcttat cagataaaag ggaaaatcaa
    30961 agtcaaaagg aatcaatcga aaaaaatcca agaaacttaa aaaaatccaa gacaaaaaaa
    31021 gtacacctaa aaaatttaaa gtaaatccca taaaaaaatc aatgttaacg ctttctttat
    31081 tagctatact cggtgtatca attgaaatgg gggataaaaa atgaaaaaca aatttagaaa
    31141 gattgttggg actatcgcta ttttgaccgc aagcattcta ttaagtgcgt gtggcaattc
    31201 aggatctgca gatgatagta cggggtatgt aggaatcgct atgccgacca aatcagcaga
    31261 aagatggatc gctgatggga acaacatggt ctcggaatta gaaaaactgg gttataaaac
    31321 agatcttcaa tatggagaag ataaagtaga aaatcaagta gcacaaatag aaaatatgat
    31381 tacaaaaggg gtagatacac ttgtgattgc ttctatcgat ggttcggctt taactgatgt
    31441 gttagctaaa gcaaaagaag cagatatcaa ggtgatcgcc tatgatcgtt tactgatgaa
    31501 ttcagaaaat gtcgattatt acgcgacttt tgataatttt ggagtaggtg tatcgcaagc
    31561 agcttatatt gaagagcact taggattgaa agaaggaaaa ggaccattca cgattgaatt
    31621 atttggtggc tcgcctgacg acaacaacgc actaatcaat tacaacggag tgatgtctgt
    31681 tttacaacca tatatggaca acggtcagtt ggtcgtgcca tctggacaga cgagtttcag
    31741 tcaaatcgct acgcttcgct gggatggttc tacggcgcaa gcaaggatgg ataatctgtt
    31801 aagcgcaaat tatactgacc aaacattgga tgctgttttg tcgccatatg atccaataag
    31861 tttaggaatc atttcgtcat taaaaggcgt ggggtatggg agtgaaagta aacctttacc
    31921 tgtgattact ggacaagatg cgacagttgc tggcgttaaa tcgatcattg ccggggaaca
    31981 aacacaaacg atctttaaag acacacgtat cttagcaaaa aatacgattg aaatgatcaa
    32041 agcaatcagt gatggagaag aggttccggt caatgatact gaaacttatg ataatggggt
    32101 gaaaactgtc cctacgtatt tagcaaatac agtatctgtc gataaagata attatcaagc
    32161 agagctgatc gacaccgatt attataaaga atccgatctg aaaaattaag aggaaccaaa
    32221 caaggaacta aagagatcta agtcagagag aggataagaa ttatggcgga ttgtatttta
    32281 aaaatggcag atatcaccaa aaaattttct ggaatccgag cgttagattg cgtgaatctg
    32341 atgattgaac gtggggaaat ccatgcattg tgcggagaaa acggagcggg aaaatcaaca
    32401 ttgatgaatg tcttgagcgg cttgtatcca tatggctcct atgacgggga aatcttttat
    32461 gatggaaagc tgtgtcaatt taaagatttg aaagatagtg aagaaaaagg catcgtcatc
    32521 attcatcaag aattagcact aagtccttat ctttcaataa aagagaatat ttttctcgga
    32581 aatgaacaag cagaaagagg cgtgattgat tgggaactga cagaaaagaa aacatatgaa
    32641 ctgctgaata aagtcggttt gtctatcgac ccgaatcttc tagtatccca gattggcgta
    32701 gggcaacagc aactagttga aatagctaaa gcattatcaa aatccgtccg tctgttgatt
    32761 ttggatgagc ctaccgctgc attgaatgaa gaagaaagcg cgaatttgct gagattgatc
    32821 cgagagttga aagagcaagg tgtaacctcc atcattattt cacataagct gaatgaaatc
    32881 gtggatatcg cagatcgaat caccatttta cgggatggac agacgattga gacactagaa
    32941 aatgaaatga tcagcgaaga gcatattatt cgcgggatgg tgggcagaga tttagttgat
    33001 cgttatcctg aaagaaaacc ggatattgga gaagtttatt ttaaagtaga agactggacg
    33061 gtctatcacc ctcttgatca cgagcgaatc ataaacgacc acatcaactt gcatttgaaa
    33121 aaaggagaga ttgtaggaat agctggactg atgggagcgg gcagaactga attcgctatg
    33181 agtctatttg gacattctta tggaagcaag atatccggac gtatattaaa ggaaggggca
    33241 gagatatcag ttaagaccgt tccttcagcc attgcaaacg gactcgctta tgtctcggag
    33301 gaccgaaaat ctttaggact gaatctgctg atggatattc gggaaaacgt gacgctgagc
    33361 agtttggata aaataagtca aaaaggagta ttagataaag aaaaagaagt aactgaggca
    33421 gaaaatttcc ggaaaaagat gcgtataaaa acaaactcca tccatcaaat tgtcagtagt
    33481 cttagtggtg ggaatcaaca aaaagtcgtt ttatccaagt ggctgatgac acaacccgat
    33541 attctttttt tggatgagcc aacccgtg
//
LOCUS       DACEEZ010000022        31835 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20313_2017
            SAMN12639966-rid9879223.denovo.022, whole genome shotgun sequence.
ACCESSION   DACEEZ010000022 DACEEZ010000000
VERSION     DACEEZ010000022.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639966
            Sequence Read Archive: SRR10023158
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 31835)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 31835)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741683.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741683.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:44:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,016
            CDSs (total)                      :: 2,962
            Genes (coding)                    :: 2,889
            CDSs (with protein)               :: 2,889
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 36 of 73
            Pseudo Genes (incomplete)         :: 29 of 73
            Pseudo Genes (internal stop)      :: 18 of 73
            Pseudo Genes (multiple problems)  :: 8 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31835
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639966-rid9879223.denovo.022"
                     /isolate="E20313_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(152..2305)
                     /locus_tag="IWO89_001111"
     CDS             complement(152..2305)
                     /locus_tag="IWO89_001111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 2"
                     /protein_id="HAQ0455881.1"
                     /translation="MMKDFMKKILNTSWLEKIRKGAQEPKNKTFRRSHVPFRLNFLFF
                     IIFTLFVALIARLGYLQIVNGEEMEARVKSTSTITVQESTPRGMIYDSTGKALVTNKA
                     NQAITYTRGTQVTAKDLLEIATKLNSLIDVPVDENLTDRDKKDFWLADPKHLKEATKR
                     LSAKEKQLGNSEQYSAMVNKVTADEINLNEEQLKIATIFKRMNSAYELNTVYIKNSGV
                     TDQELAVVAERASELPGVSTGTDWTREYNAASSLKSILGSVTTEKQGLPADEAEKYLA
                     KGYSRNDRVGQSYLEKQYEDVLQGTKTQYEVSLDNEGNVSNQKEIFSGEKGSNLMLSM
                     NAEFQSKVEEILKRNYQTLINNGKAQYSPGAYAVAMNPQTGEVLAMTGFSHEQGSKEI
                     TENALGTITSAFDPGSVVKAGTLTAGWASNAISGNQVLIDEPIRLQGASEKSSVFNRS
                     GQVALDAVKASELSSNTYMIKVALKMLGLDYTPGMGLPSLDEEAKAYQQLRDSFKEFG
                     LGTTTGIDLPNESPGISRSVDYMKKFNADNGKEWYAPGNFTDLAFGQFDTYTPIQLAQ
                     YASTVANGGKRVQPHLVKAIYGNDENGNLGEVKKEIGTTVENTVNISAENMAILREGF
                     HQVVHGTDPYTTAKPLASAKMDLSAKTGTAETVAEGHPDIATVNSNIVAYGPTDNPEI
                     AISVVLPNLLDEQDHINLTIAKEIMDAYYDMFMAK"
     gene            complement(2599..3447)
                     /locus_tag="IWO89_001112"
     CDS             complement(2599..3447)
                     /locus_tag="IWO89_001112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HAQ0455882.1"
                     /translation="MSVISRIESMMEDYSSAEKKLANYIIGNIEKVPTMTANELAEAS
                     GLSAPTVVRFSKKIGFQSLTDFKITISTELQTGIDEGFSDIEANESFYSIKNKLGNNA
                     QVAIKETVDILEEETIQKVVECLESAETVFLYGVGASSLVVEDILQKWSRVGKPIIFE
                     KDIHVLLPQLVSNEKKKVLWLVSNSGRSADVVALAELAKSMNIEIIALTQFGNNPLSK
                     IADVLVQTSRPKEITNRSAATNSLLAQFATIDIIFYFYMAKNEKLSEKVKKTREAIQE
                     YFMDSK"
     gene            complement(3671..5611)
                     /gene="thrS"
                     /locus_tag="IWO89_001113"
     CDS             complement(3671..5611)
                     /gene="thrS"
                     /locus_tag="IWO89_001113"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HAQ0455883.1"
                     /translation="MIKITFPDGAVKEFESGTTTLAIAESISKSLAKKALAGKVNGKL
                     VDLTRPIEEDASIEIITPDHEDALGLVRHSAAHLMAQAMRRLYPNIHFGVGPAIDSGF
                     YYDTDNGQNQVTAEDLPAIEAEMMKIVKENLPIERRVLSKQEALEIFASDPYKVELIS
                     ELPEEEVITAYQQGEFIDLCRGPHVPSTGRIQVFKLLSVAGAYWRGNSNNQMMQRVYG
                     TAFFDKKALKEYIRLREEAKERDHRKLGKELDLFMVSPEVGSGLPFWLPKGATIRRTI
                     ERYIVDKEVSLGYQHVYTPIMGDVELYKTSGHWDHYQEDMFPPMDMGDGEMLVLRPMN
                     CPHHMMVYKNTIHSYRELPIRIAELGMMHRYEKSGALSGLQRVREMTLNDGHTFVRPD
                     QIKDEFKRTLELMVAVYADFNITDYRFRLSYRDPNNTDKYFDDDAMWEKAQAMLKAAM
                     DELELDYFEAEGEAAFYGPKLDVQVKTALGTEETLSTIQLDFLLPERFDLTYVGEDGE
                     NTHRPVVIHRGIVSTMERFVAYLTEVYKGAFPTWLAPIQATIIPVSVDAHGDYAYEIK
                     ERLQMKGLRVEVDDRNEKMGYKIRASQTQKIPYQLVVGDKELEDATVNVRRYGSKETA
                     VEDLNIFIDAMEAEVKNYSREN"
     gene            complement(5948..6127)
                     /locus_tag="IWO89_001114"
     CDS             complement(5948..6127)
                     /locus_tag="IWO89_001114"
                     /EC_number="5.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-oxalocrotonate tautomerase"
                     /protein_id="HAQ0455884.1"
                     /translation="MPFVHVELVEGRSPEQLENMMKDITEAVHKNTQAPKEHIHVIIN
                     EMKKGTYGVNGEWKK"
     gene            complement(6174..7997)
                     /gene="cadA"
                     /locus_tag="IWO89_001115"
     CDS             complement(6174..7997)
                     /gene="cadA"
                     /locus_tag="IWO89_001115"
                     /EC_number="7.2.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cadmium-translocating P-type ATPase"
                     /protein_id="HAQ0455885.1"
                     /translation="MTHFKKFLLTLLIGGIALLSEFVFHQPALAFAIVAFTGGILAFL
                     MFLEMIKTLRSGRYGVDVLAITAILATLAVKEYWASLMILIMLTGGDSLEDYANKRAG
                     KELQSLLDNTPRIAHRFKEGKLQDIVIDEINIDDQLLVKPGELVPADGRVTKGESSVD
                     ESSLTGESRPETKKPGDQIMSGSLNGDGSIQMIAEKRAADSQYQMIIKLVEESKEKPA
                     RFVRLADRYAVPFTLIAYLIGGISWWFSKDPLRFAEVLVVASPCPLILAAPIALVAGM
                     SRSSKNGIVVKTGTTIEKLARTKTIAFDKTGTLTKGILEVEAIQPVNGIKKEELLLYA
                     ASAEQESSHILARSLVNNVKNSQLYPVSQLKEVTGQGIQAVVNNHEVKIGRASFVSAE
                     EIETSNTAIYVTIDRSFAGTILFSDQIRPEAAKTIKELRKLDVKNLMMITGDGSAIAQ
                     TIAEKVQLDSVHARSLPQDKLTILESIPKDQRPVAMVGDGVNDAPALAAADIGIAMGA
                     HGSTAASESADAVILKDDLLRVAKAVVISRDTMRIAKQSVLIGIFICIFLMLIASTGL
                     IPALFGAVLQEVVDTVSILSALRAKKDPAIFPDSVPKTNIV"
     gene            8275..10554
                     /locus_tag="IWO89_001116"
     CDS             8275..10554
                     /locus_tag="IWO89_001116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HAQ0455886.1"
                     /translation="MNEKQLEQTHVDETIELIQIEQNMLDTKQQELTQEMNHSTKDTA
                     NHKIRAGSNEAFYESAVEYRQHEQELLLKYHTLEAQQKRLQTLSVMKDSPYFARIDFK
                     EDRETETLYLGIASLRDLAEETIVIDWRAPIANLYYEGEIGPAFYETETDRIDVDLQL
                     KRQFKIQEGRILSMVDTSEVINDDFLLEILDESSSNHMKNIVSTIQKAQNAIIRDTTS
                     KVMLIEGIAGSGKTSALLQRIAFLLYHNRKWLEAENVLLFSPNHLFSDYISTVLPSLG
                     ESGVPTQTFKNYLVQLLPEFSLLDEQQQESGFLLGEKDPIQVMKSGLTLVDQIDRYIQ
                     GITNYGPLFRDMKINGRTILSKESIRQWYKETNEQLPLHHRLSLLQTKLLKKLGGLQK
                     DETRQQWVKDLAEEQLQELYATDPNLEYTEQKERSLRKKLANQIVRKRFRRIHRGIMN
                     YQFVNITKQYLHFLQTVPLSLLTRHKISETRWQEHLTTVRTHLRQRELRQEDAVLFFL
                     LMRRTYPVTVTHKARYIFIDEMQDFAPSQVALLQALFPKANLTFCGDLNQNVFGNETI
                     VGSLEDLFVDREVTRFQLTTSYRSTKEITDFANHFIVKEHQVETTARKGDLPLLIQGT
                     TDDIKLNWLADTIRTTQGKARYWRTAIIAKTQEECEALYAQLPDELKQQVQLILNESD
                     FMKRSIILLPAFLAKGLEFDRVFLWDVDAEHFSSQQDKLILYTMCTRAMHELTLFTSN
                     NESPLLNNMDISSYEKKVL"
     gene            complement(10647..11294)
                     /locus_tag="IWO89_001117"
     CDS             complement(10647..11294)
                     /locus_tag="IWO89_001117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0455887.1"
                     /translation="MEAKITLEPFERILSGYRKVEELAVNVTDCSKLAQKYARFGVEG
                     YRLGNYVGTGYLNRYLECMVDRAPMLIYRQNYLIPLLFRRSDSAFRLFEEEYRMEAFF
                     LLLEWSLKHRPEKILIERNEKIDTKKNKVIDSAYLAFRVSEILDCGGYPISNFQSIDQ
                     FIEWNRIYRLIDNGGIGRHSKVFDPEYPENMEELKMIISLVKLKYPETDLDLYIE"
     gene            complement(11473..12192)
                     /locus_tag="IWO89_001118"
     CDS             complement(11473..12192)
                     /locus_tag="IWO89_001118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HAQ0455888.1"
                     /translation="MGRKWANIVAKKTAKDANNSRIYAKFGIEIYAAAKSGDPDPHAN
                     QKLRFVIDRAKTYNVPKHIIDRAIEKAKGAGDETYSELRYEGFGPSGSMVIVDTLTNN
                     VNRTAADVRAAFGKNGGNMGVSGAVSYMFDNTAIFAFAGEDADEILEYLMEKDIDVRD
                     VMEEEGQIIVYGEVADFHSIQEALKEKGITDFSIAEIQMIPQNEVTLSPEDQETFEKM
                     IDALEDLEDVQHVFHNVALED"
     gene            complement(12323..13474)
                     /locus_tag="IWO89_001119"
     CDS             complement(12323..13474)
                     /locus_tag="IWO89_001119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase"
                     /protein_id="HAQ0455889.1"
                     /translation="MKSCGIIVEYNPFHNGHRYHVEMARKITGAEVVIAVMSGNFLQR
                     GEPAIIDKWHRANEALQNGVDLVVELPVEWAVQSADYFAKGAVKILQTLDCDSLCFGT
                     EEETSFDYSSFGRFVQENQDRIEEAFQELSDQTYSYPQKMTEVFRSLYPDITLDFSSP
                     NHILGLSYAKENAKYVHPMELHPIRRKSAGYHEKIITDQRIASATAIRHALEEGKRIA
                     GLVPPQTEEDLSTCSLQTWESYWPYLRYRLLSSTLLELTDIYQMTEGIESRMKTAAAT
                     ADSFEDFVSKVKTKRYTWTRIQRLSCYVLLNIKKQEIPVQQNKNYLRLLGFTDAGRRF
                     LKEKKEISIYSRIGKKEAQEAELLVRSDQIYQLGGNIPEQNFGQIPIRV"
     gene            complement(13691..14431)
                     /locus_tag="IWO89_001120"
     CDS             complement(13691..14431)
                     /locus_tag="IWO89_001120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HAQ0455890.1"
                     /translation="MAYETFAFIYDEVMDETLYQQWLAFSKRHLQEDTKNILELACGT
                     GALAVAFAKDGYKVTGLDLSEEMLMIASQRAYEEDASIQFVEGNMLDLSEIGQYQAIT
                     CFSDSLCYMKNRQEVQQVFDEVYQALEENGRFLFDVHSTYQVDEVFPEYSYHYQGDNF
                     AFLWDSYPGSEKHSIEHFLTFFVEEQEGSGRFIREDELHQERTYPLESYLRMLENSGF
                     SKVEAFSDFTDEAASEKSKRWFFVAYKD"
     gene            complement(14435..14758)
                     /gene="rsfS"
                     /locus_tag="IWO89_001121"
     CDS             complement(14435..14758)
                     /gene="rsfS"
                     /locus_tag="IWO89_001121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HAQ0455891.1"
                     /translation="MEAADSKRAEDIIALDVRNVSLLADYFMICSANSERQINAITEE
                     IIDKEEENKYEVKRIEGKEGGKWVLIDLGDVIVHVFHAPERNFYNLEKLWSDAPLVDL
                     SEWLD"
     gene            complement(14815..15408)
                     /locus_tag="IWO89_001122"
     CDS             complement(14815..15408)
                     /locus_tag="IWO89_001122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HAQ0455892.1"
                     /translation="MNMDYSNEYTAYSRENLMQKIQMRMSEQRFKHVLGVEETAVALA
                     KKYGASPEKASIAALTHDYAKERPDEEFKMVIVRDGFDPELLNYNNAIWHGLVGASFV
                     ERELGITDAEILHAIRVHTTGAAKMSLLDKIIYVADYIEPGRDFPGVQDARAIAWADL
                     DEAVAFETKHTLAHLLAQEQQIYPKTIETYNYWVAKK"
     gene            complement(15392..16039)
                     /locus_tag="IWO89_001123"
     CDS             complement(15392..16039)
                     /locus_tag="IWO89_001123"
                     /EC_number="2.7.7.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide adenylyltransferase"
                     /protein_id="HAQ0455893.1"
                     /translation="MNTQAKTFVRSQLFPEEMPQFLEKKKQVGILGGTFNPVHLAHLV
                     MAEQAGRNLGLDRVFLMPSYQPPHVDEKQTIDAKHRLNMLELAVEDNPFLQIETIELA
                     RGGKSYTYDTMKELTQNNPDTDYYFIIGGDMVEYLPKWYKIDELTSMVNFVGIRRPGY
                     TTDTPYPVIWVDVPEIDISSTKIRQKIKEGCSIRYLVPDKVIDYIQNEGLYEYGL"
     gene            complement(16052..16363)
                     /gene="yhbY"
                     /locus_tag="IWO89_001124"
     CDS             complement(16052..16363)
                     /gene="yhbY"
                     /locus_tag="IWO89_001124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome assembly RNA-binding protein YhbY"
                     /protein_id="HAQ0455894.1"
                     /translation="MSLRGKQKRFLRSKAHHLQPIFQIGKGGINSAMIVQIEEALEKR
                     ELIKVSLLQNTDEIAEEAAQVLEKEIDCEIVQIIGRVIVLYKPSTKEKYQRLSKEVKA
                     I"
     gene            complement(16379..17488)
                     /gene="yqeH"
                     /locus_tag="IWO89_001125"
     CDS             complement(16379..17488)
                     /gene="yqeH"
                     /locus_tag="IWO89_001125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase YqeH"
                     /protein_id="HAQ0455895.1"
                     /translation="MTEELQCIGCGAVIQTNKPGELGYTPQTALEKGLASGEVYCQRC
                     FRLRHYNETQDVSLTDDDFLRLLNELGQKDALIVNVVDIFDFNGSLIPGLHRFVGDNP
                     VLLVGNKVDILPKSLKKTKLTQWMKERAYEAGIRPVDVLLTSAKKSHEMTELLEKIEE
                     YRDGRDVYIVGVTNVGKSTLINQIIKNTAGVQDVITTSQFPGTTLDKIEIPLDDGHFL
                     IDTPGIIHRHQMAHYLGKKDLRIIAPLKEIKPKVYQLNEGQTLFLGGLARFDFISGNR
                     ASFIAYVSNDVNIHRTKLEKADEFYQKHVGGLLQPPRPDEVEAFPELVRFEFSVKEKT
                     DIVFAGLGWITVTAPVVIAGWAPKGVDVLKRKALI"
     gene            complement(17485..18012)
                     /locus_tag="IWO89_001126"
     CDS             complement(17485..18012)
                     /locus_tag="IWO89_001126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqeG family HAD IIIA-type phosphatase"
                     /protein_id="HAQ0455896.1"
                     /translation="MFSNYKPTWMVEAIYQITPAQLKNLGIKAVLTDLDNTLIAWNNP
                     DGTEELLTWILEMKNAGIPVVVVSNNNSKRVARAIEKFDLLYVARAMKPLARGINIAK
                     KQLDLADDEIVMVGDQIMTDIRGANRAGIRSILVKPIIATDSWKTQFNRFWEKRIMAY
                     LLAKHPDMGWRGGIE"
     gene            complement(18192..18977)
                     /locus_tag="IWO89_001127"
     CDS             complement(18192..18977)
                     /locus_tag="IWO89_001127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyl transferase
                     subunit alpha"
                     /protein_id="HAQ0455897.1"
                     /translation="MDKTAHDVVQLARAKERWTSLDFFEEIFDSFEECHGDRLFADDE
                     AIVGGIARLDGKPVTVIGIQKGRDLQENIRRNFGSPHPEGYRKALRLMKQAEKFNRPV
                     ITLINTAGAYCGVGAEERGEGEAIARNLLEMSDLKVPIIAIIIGEGGSGGALALGLAD
                     EVWMLEETIYAVLSPEGFASILWKDGSRAAEAAELMKITAPELRELGIVDRVIPETFN
                     GEQLPRYKVAHIMKKALIERLTELVRLDQHKLLEKRYKRFRQY"
     gene            complement(18970..19839)
                     /locus_tag="IWO89_001128"
     CDS             complement(18970..19839)
                     /locus_tag="IWO89_001128"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyltransferase
                     subunit beta"
                     /protein_id="HAQ0455898.1"
                     /translation="MALFKKKKYIRINPNRESKPANSPSVPDNMWAKCPNCKHILYTK
                     DIGEEKVCPHCGYAFRIGAWQRLALIIDEKSFEEWDTDLVTKDPLAFPEYTEKIKKMQ
                     DKTGLHEAVLTGKATIQGIPFIIGVMDPNFIMGSMGTIVGEKITRLFERATKESLPVV
                     LFTASGGARMQEGIFSLMQMAKISAAVKRHSNEGLFYLTVLTDPTTGGVTASFAMEGD
                     IILAEPQSLIGFAGRRVIEQTIKQELPEDFQKAEFLLSHGFVDQIVPRMELKQKIHTL
                     LELHTQKGWIDRG"
     gene            complement(19840..21219)
                     /locus_tag="IWO89_001129"
     CDS             complement(19840..21219)
                     /locus_tag="IWO89_001129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HAQ0455899.1"
                     /translation="MFSKVLIANRGEIAVRIIRACRELGIQTVAIYSEADAEALHTQL
                     ADEAICIGPAKAVDSYLNVQEVLSAAIVTKAEAIHPGFGFLSENSRFASMCEECNITF
                     IGPKSATIDAMGNKINARQLMQEANVPVIPGSTGVLSTVEEALEIADRIGYPVMLKAA
                     AGGGGKGIRKVQSKEELPQHFSSAQQEAAAAFGNDDMYLEKIIYPARHIEVQILGDRF
                     GHVIHLGERDCSLQRNNQKVLEESPSVVISQTKREALGDAAVRAAKAVNYENAGTIEF
                     LMDEEGDFYFMEMNTRIQVEHPVTEMVTGIDLVKKQVEIAAGEPLNVCQEEVVFQGHA
                     IECRINAENPAFHFAPSPGKIQNLLLPAGGMGLRVDSAVYPGYTIPPYYDSMIAKIIV
                     HGNTRFEALMKMQRALSEFITEGIITNAEFQMDLISHPAVIAGDYSTAFLQEEFLPNW
                     TPETEIGGA"
     gene            complement(21227..21646)
                     /gene="fabZ"
                     /locus_tag="IWO89_001130"
     CDS             complement(21227..21646)
                     /gene="fabZ"
                     /locus_tag="IWO89_001130"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HAQ0455900.1"
                     /translation="MNIQEIKEIIPHRYPMLLIDRVEEMVEGERIVAKKNVTINEPFF
                     QGHFPEEPVMPGVLIVEAMAQAGAVALLSLEQFKGKTAYFGGLDKAKFRKKVTPGDTL
                     YLEVEILKVKASAGIGKGIAKVDGKKVAEAELTFMIG"
     gene            complement(21671..22147)
                     /gene="accB"
                     /locus_tag="IWO89_001131"
     CDS             complement(21671..22147)
                     /gene="accB"
                     /locus_tag="IWO89_001131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein"
                     /protein_id="HAQ0455901.1"
                     /translation="MDINEIRELVSQFDQSSLTEFDLREGQFELYMNKNKTSRGMNMV
                     QTNETLPVSEPVNVIPEQATTAPEIMKEKESAVFDGIEVVSPIVGIVYLQPAPDKPAF
                     KKVGDQVAKGEVICIIEAMKLMNEITSEADGVITEILVENESVVEYGQPLFRIQQK"
     gene            complement(22150..23385)
                     /gene="fabF"
                     /locus_tag="IWO89_001132"
     CDS             complement(22150..23385)
                     /gene="fabF"
                     /locus_tag="IWO89_001132"
                     /EC_number="2.3.1.179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HAQ0455902.1"
                     /translation="MNRVVITGYGVTSPIGNDADSFLNSLKEGTNGIAPITKFDASET
                     GITLAGEVKDFPYDKYFIKKDSKRMDMFSIYGIYAALEAMEMSGLDKEKMNQDRFGVI
                     IGSGIGGLPTIENQVIRLHEKGAKRVSPMFVPMAIANMAAGNIALRVGAKGICTAIVT
                     ACASGTNSIGEAFRNIKHGYSDVILAGGTEATICEMGIAGFAALTALSESTDPNRGSI
                     PFDENRNGFVMGEGAGVLVLESLEHAQARGAKIYGEIVGYGANCDAYHMTAPTPDGSG
                     ASKAMKLAMEEAGVQPEQVGYINAHGTSTPANDQAEAKAIQIALGENFRDTYVSSTKS
                     MTGHLLGAAGGIEALATLLALEHQFIPPTINVEKQDPEIDLTVVKNESKPASFTYAMS
                     NSLGFGGHNAVLCMKRWEE"
     gene            complement(23404..24141)
                     /gene="fabG"
                     /locus_tag="IWO89_001133"
     CDS             complement(23404..24141)
                     /gene="fabG"
                     /locus_tag="IWO89_001133"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-[acyl-carrier-protein] reductase"
                     /protein_id="HAQ0455903.1"
                     /translation="MDVKGKNVFITGSTRGIGKAMALAFAKAGANIILNGRGEIPKEK
                     IEEIEAFGVKCVGVSGDISDYEKAGQMIKEAEEKLGSIHVLVNNAGITNDKLVMRMDA
                     EDFKKCLDINLIGTFNMTQHVLKKMMKQREGAIINLSSVSGLIGNIGQANYAASKAGV
                     VGLTKSVAREAATRGITCNAIAPGFITTDMTEVLADKVKEQAEKQIPMQRFGQVEDIA
                     QTAVFLAQNPYITGQVINVDGGLVMHG"
     gene            complement(24153..25070)
                     /gene="fabD"
                     /locus_tag="IWO89_001134"
     CDS             complement(24153..25070)
                     /gene="fabD"
                     /locus_tag="IWO89_001134"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HAQ0455904.1"
                     /translation="MKTAFLFSGQGAQYQGMGKDLYEEAVVKQTFDEASEILGYDMAE
                     LCFTENERLDQTQYTQPAILTVSIAYYRLLKEHGIIPDAALGLSLGEYSALVASNALS
                     FTEAVALVAKRGAYMTEAAPAGSGKMVAVMNAPIETIEESCHEASKYGIVSPANYNTP
                     QQIVIGGEEKAVDEAVLLLKEKGFKRMIPLNVSGPFHTAILEPAAKKLAKDLSQIQFS
                     EPSFPIISNTTTEIMKKETIAGLLEQQVMQPVRFYESIHKLKTIGIEQVIEVGPGKVL
                     SGFMKKIDKTIPVLRVENKQTFDETIAIL"
     gene            complement(25177..25401)
                     /locus_tag="IWO89_001135"
     CDS             complement(25177..25401)
                     /locus_tag="IWO89_001135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HAQ0455905.1"
                     /translation="MVFEKIQEIIAEELGKETEEIKLTTDIQEDLEADSLDLFQIINE
                     IEDEFDVKIETEDGIKTVQDLVTYVEKQTA"
     gene            complement(25454..26416)
                     /locus_tag="IWO89_001136"
     CDS             complement(25454..26416)
                     /locus_tag="IWO89_001136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketoacyl-ACP synthase III"
                     /protein_id="HAQ0455906.1"
                     /translation="MEQFGMITATASVLPEKIITNNDLSKMMDTSDEWISSRTGIRER
                     RCVTNQETSDLCILVAEKLIKQKNLNPKDLDFILVATMSPDYTTPSVAAQVQGKLGAT
                     HAIAFDISAACSGFVYALSMAEKMIRCGSTKGLVIGGETLSKLIDWSDRTTAVLFGDG
                     AGGILLEANPQKKLLKEKLAADGTRSSSLTAGYQQNKNPFYSEDSEGSYYLQMTGRDI
                     FDFAVRDVANNIQEVMKDKPVDYLLLHQANLRIIEKIARKVKMPQEKFLTNMDKYGNT
                     SAASIPILLDEAVSSGKITLGKQQKVIFTGYGGGLTWGSILMEL"
     gene            complement(26426..26866)
                     /locus_tag="IWO89_001137"
     CDS             complement(26426..26866)
                     /locus_tag="IWO89_001137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HAQ0455907.1"
                     /translation="MEPDLKTVNDYLVSVFNDILTIEESELKKSQFKDLSITEMHTIE
                     AIGMYKKKTTSEVAKELSITVGTLTTAINKLVKKGYVERIRSEDDRRVVKLGLTKKGK
                     LLFRVHQHFHREMVKNILDGMATEEQHALLDALKNLHDFLQDYK"
     gene            27172..28137
                     /locus_tag="IWO89_001138"
     CDS             27172..28137
                     /locus_tag="IWO89_001138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HAQ0455908.1"
                     /translation="MLKEKMKTLPMLPGVYLMKDRDGKVIYVGKAKRLKNRVSSYFHQ
                     NKQHSKKVLRMIHHITDFDFVVVDTELDALLLECQLIQHYRPFYNRQMNYFSNYNYVH
                     ITNKGFVLTDTPTARTYGPFRLYKKMPSILRIMEETYQMPWLSEISLLALRVQLPDLQ
                     EMTFEQKKKELQGLFQGRNKKLLTYLKKRQQHFIYQLNFEKAGMLQKDIELVTYFIRR
                     IQEQKQFLRTPSLTFSMPLAADESQKKHYLVCYGQLAETMITSGDVSPDFYYEKKEAH
                     LSLKRQLSKEEIDPVQILISYRKKLEKEQFEIELLNKKEAEKQLN"
     gene            complement(28194..28424)
                     /locus_tag="IWO89_001139"
     CDS             complement(28194..28424)
                     /locus_tag="IWO89_001139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016175387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YkuJ family protein"
                     /protein_id="HAQ0455909.1"
                     /translation="MTHSQLVAIIKRLEAMVESADNELQVRRFEKEGVEKCTVTYDKA
                     TDTFELTETDTHQQYEFDNIDIVAMEIYDLIQ"
     gene            complement(28510..29397)
                     /locus_tag="IWO89_001140"
     CDS             complement(28510..29397)
                     /locus_tag="IWO89_001140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HAQ0455910.1"
                     /translation="MKLGFIGTGVMGSAVARHLLEAGHEVAVYNRTKAKADPLVTEGA
                     IWADTPKAVAEQSNILFTMVGYPKDVEEIYYGQSGIFSADISGHILVDLTTSTPSLAE
                     KIAKTAKEKGADALDAPVSGGDLGAKNGTLTIMVGGEEAVYDQVLPLFKEFGTTFTLH
                     GSAGKGQHTKMANQIMIAGTMTGMTEMLVYAQKNGLDLKKVIETLSGGSAANWSLSNY
                     SPRILKEDYTPGFFVKHFIKDLKIALEEAEKMDLVLPATTQALKLYEELADKGFENDG
                     TQALIKLWWPEGKIPEKKS"
     gene            complement(29486..30709)
                     /locus_tag="IWO89_001141"
     CDS             complement(29486..30709)
                     /locus_tag="IWO89_001141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HAQ0455911.1"
                     /translation="MKIGFFTDTYFPQVSGVATSIKTLKQELERHGHEVYIFTTTDPN
                     ADKLEKDVIRMPSVPFISFKDRRVVVRGMWYAYLVAKELELDLIHTHTEFGAGILGKM
                     VAKKLKIPLIHTYHTMYEDYLHYIGKGKVIRQSHVKYLSRLFANHTTGVVCPSERVID
                     TLRSYGVIAPLRVIPTGIDIEKFKRSDITQQDISKLRESLGLQENHLMILSLSRISYE
                     KNIQALINGFPQVISAYPNARLVIVGKGPYVEELEELISELQLEEFVQFTGEVDNNDV
                     ALYYKAADYFVSASTSETQGLTYTEAMAAGTPCVVEGNAYLNRLFDHPSLGKTFETDE
                     DFAPALIDYISSEVPKDPKVLEAKLYDISSTHFGEAMIEFYQDMVVYHEEEMRRKEEE
                     DSIERIKIKLNSFKR"
     gene            complement(30726..31805)
                     /locus_tag="IWO89_001142"
     CDS             complement(30726..31805)
                     /locus_tag="IWO89_001142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HAQ0455912.1"
                     /translation="MLYYISEGGRKVKVLLYFESEKMLAKSGIGRALDHQKRALTEVG
                     ISYTLDEKEDYDILHINTYGINSHNMVNKARREGKKVVYHAHSTEEDFRNSFIGSNQL
                     SPIVKKYLVGLYQKADYLITPTPYSKQLLESYGIRVPIQAISNGIDLEKYHPDPMKEQ
                     KFREYFKLSPDQKVIICVGLFFERKGIIDFVEIAKKMPEYTFIWFGHVPMYSIPRNIR
                     KIVKEDHPENVLFPGYIRGEIIEGAYSGADLFFFPSYEETEGIVVLEALASKQNVLVR
                     DIPVYNGWLEDSKNCYMGKTNEEFIRLIQQITNQELPSTTQAGYQTAKERSINKIGEE
                     LKNVYESVLNEKVSSKLKKVNQVKD"
BASE COUNT         8793 a         6970 c         5488 g        10584 t
ORIGIN      
        1 caatttttgt cacaaccttt tttgccgagt aaacggtgtt caaaagccag tttctcggta
       61 tgaagtcaaa tttaagcaaa aatgtgagaa gctattttca caaaattttt cttcatactc
      121 gaaactaagc ggctttttca cgacctcttt tttattttgc catgaacata tcataatacg
      181 cgtccattat ttctttagca atcgtcaagt ttatgtggtc ttgttcgtct aataagttcg
      241 gcaagacaac actgatggca atttccggat tgtctgttgg tccgtatgcc acgatattac
      301 tattgactgt cgcaatatct ggatgacctt ccgcaaccgt ttctgcagtc cctgtttttg
      361 cggaaaggtc cattttcgca ctggctaatg gtttggctgt tgtgtaagga tcggtaccat
      421 ggacaacttg gtggaatcct tcacggagga tcgccatatt ttctgcagaa atattgaccg
      481 tattttctac tgttgtgcca atttctttct tcacttcacc caagtttcca ttttcgtcat
      541 tgccatagat cgctttgact aggtgcggct gtactcgttt cccgccatta gcgactgttg
      601 aagcatattg agctaattgg attggtgtat atgtgtcaaa ctgtccaaat gccaaatcgg
      661 taaagttacc tggagcatac cattctttgc cattatccgc attgaacttc ttcatataat
      721 caacagaacg tgagatccca ggagattcat tcggcaaatc gatcccagtc gttgtaccaa
      781 gaccaaactc tttgaaagag tctctcaatt gctgataagc tttagcttct tcatctaaac
      841 taggtagtcc cattccaggt gtatagtcta gtcctaacat ctttaacgcc actttgatca
      901 tatacgtatt agaagacaat tccgatgctt tgactgcatc aagagcaact tgtccgcttc
      961 gattaaagac agagcttttt tctgaagcac cttgcagacg aatcggctca tcaatcaaaa
     1021 cttgatttcc gctgatcgcg ttacttgccc agcctgctgt caaagtacca gccttgacta
     1081 cagaccctgg atcaaaggcg ctagtaatcg tgcctaaagc attctcggtg atttctttgg
     1141 aaccttgttc atgactgaaa ccagtcattg ctagcacttc tcctgtctga ggattcatag
     1201 ccactgcata agcaccaggc gaatactgtg ctttgccgtt gttgatcaat gtttgataat
     1261 tccttttcaa aatttcttct acttttgatt ggaattctgc attcatagat aacattaaat
     1321 tagaaccttt ttccccagag aagatttctt tttgattcga aacatttcct tcattatcaa
     1381 gggaaacttc atattgggtt ttggttcctt gcaggacatc ttcatattgt ttttccaaat
     1441 aactttgtcc tacacggtca ttccgagaat aaccttttgc taggtatttc tctgcttcat
     1501 ctgctggaag tccttgcttt tcagtcgtca cactgccaag aatacttttc agtgaacttg
     1561 ctgcattata ttctcgtgtc cagtctgtgc cagtcgaaac tcctggaagt tcagaagcgc
     1621 gttctgccac aacagctaac tcttgatcag taacaccaga gtttttgata tagacagtgt
     1681 ttaattcata tgcactattc atccgtttaa agatggtagc aatttttaat tgctcctcgt
     1741 ttaaattaat ctcatcagct gtgactttat tgaccatcgc actatattgt tcgctgttcc
     1801 ctaattgctt ttcttttgcc gaaagtcttt ttgtggcttc tttcaaatgt ttgggatcag
     1861 ccagccaaaa atctttttta tctctatctg tcagattttc atctaccgga acatcgatca
     1921 gcgagttcag cttcgttgcg atttcaagca aatctttagc cgtaacttgt gttcctctcg
     1981 tatatgtgat ggcctgattc gctttattcg taactaaagc ttttcctgtg gagtcgtaga
     2041 tcattccgcg aggagtactt tcttggaccg taatcgtaga agtagacttc actcgcgcct
     2101 ccatctcttc gccgttcacg atctgcaaat aacccaatct agcaatcaac gcaacaaata
     2161 acgtaaatat aatgaaaaaa agaaaattta agcgaaaagg aacatgggac cggcgaaacg
     2221 ttttgttctt aggttcttgt gcaccttttc ttattttttc taaccaagaa gtatttaata
     2281 tttttttcat aaaatctttc atcatttcct atccttccgc ctatttacat tcatgcttca
     2341 ttgtacccaa aaacttctta ataaaaaaga aaaaatgggc ggagcaagaa attctttacc
     2401 taatttcaag ataaaatcaa ctttttgttt cacagcaatc acttaaagtt acatatccaa
     2461 ctattttttt cattaaaaaa taaacttatt tttgaaaatt atgttttctt aagtcgtgaa
     2521 acactttgat ggatcattta tttgtggaaa actacgtttt gttattctaa aaaacttata
     2581 taaaaaatag taaggttttt atttcgaatc cataaaatat tcttggattg cttctcgtgt
     2641 ttttttcact ttttcgctaa gcttttcgtt tttagccata tagaaataaa aaatgatatc
     2701 gatcgtagca aattgggcca aaagtgaatt agttgccgca cttcgattcg tgatttcttt
     2761 tggtctagat gtttgtacga gtacatcggc tatcttgcta agcgggttat ttccaaattg
     2821 cgtcaacgca atgatttcga tattcatgct tttcgccagc tcggctaatg cgactacatc
     2881 tgcacttcta cctgagtttg aaacaagcca aagcactttc tttttctcat tgctgacaag
     2941 ttgcgggagc aagacgtgga tatctttttc aaaaatgatt ggcttaccga cacgagacca
     3001 tttttgcaga atatcttcta ctactaaaga agaggcgcca accccatata aaaaaacagt
     3061 ctctgccgat tccaaacact ctacgacttt ttggatcgtt tcttcttcta gaatatctac
     3121 tgtttctttt atcgctactt gtgcattgtt accaagtttg tttttgatag agtaaaagga
     3181 ctcatttgct tctatatcac taaaaccttc atcgattcca gtctgtagtt ctgttgaaat
     3241 cgtgattttg aagtccgtca gactttgaaa accgattttt ttggaaaaac gtacaactgt
     3301 cggcgcactc aacccggaag cttctgctaa ttcattagct gtcattgtag ggaccttctc
     3361 aatattaccg ataatgtagt tggccaactt tttttctgct gaagaataat cttccatcat
     3421 actttcaata cgactaatta cgctcataag ttcctccacc cttttaatga tagtctatgt
     3481 aagtatacca tatcagaccc ttattcacgg tcgtatactg gataaaaaac aaaaaaacaa
     3541 tccgcccaac agaatatctt atcccatcaa tcccacagat tctattgagc atctgtgtct
     3601 ttgaggaata cctgttatct attgttcgga ttgctttcta tctactactt ttttacagga
     3661 aatttaccta ttagttttct ctgctgtaat ttttgacttc tgcttccata gcatcaataa
     3721 agatattcaa atcttctaca gctgtttctt tacttccata acgacgtacg ttcactgttg
     3781 catcttccaa ttccttgtct ccaacaacta attggtaagg gatcttttgt gtttgagagg
     3841 cacgtatctt atagcccatt ttttcattac gatcatcaac ttctacacgc aaccccttca
     3901 tttgcaaacg ttctttgatt tcataagcat aatcgccatg tgcatcaaca gaaacaggga
     3961 tgattgttgc ttgtataggt gccaaccatg ttggaaaagc gcctttgtat acttctgtta
     4021 aataagcgac aaaacgttcc attgtagaaa caattccgcg gtggataact actggacgat
     4081 gcgtattttc cccgtcttct ccaacatatg tcaaatcaaa gcgctctggc aataagaagt
     4141 ctaattgtat agttgataat gtttcttctg taccaagagc tgttttgact tgaacatcta
     4201 gttttggtcc gtaaaaggct gcctcgcctt ctgcttcaaa gtaatctagt tctaattcat
     4261 ccatagcggc tttcagcatt gcttgggctt tttcccacat tgcgtcatca tcaaaatatt
     4321 tatctgtatt gtttggatcg cgatagctca agcggaaacg ataatctgta atattgaagt
     4381 cagcatatac tgccaccatc aattcaagcg tacgtttaaa ttcatctttg atttgatcag
     4441 gacgaacaaa tgtatgacca tcattcaacg tcatttctcg tacacgttgt aaacctgaca
     4501 aagcacctga tttttcatag cgatgcatca ttcctaattc agcgatacga attggtaatt
     4561 cacgataaga atggatcgtg tttttgtaga ccatcatgtg atgagggcag ttcattgggc
     4621 gaagaacaag catttcgcca tctcccatat ccattggcgg gaacatgtct tcttgataat
     4681 gatcccagtg tccagatgtt ttgtacaact caacatctcc cattattggt gtatatacat
     4741 gttggtaacc tagactaact tctttgtcta cgatataacg ttcgatcgtg cgtcggattg
     4801 ttgccccttt tggaagccag aatggcaaac cagaacctac ttctggggaa accatgaaca
     4861 agtcaagttc ttttcctagt ttacgatggt ctctttcttt tgcttcttca cggagacgga
     4921 tatattcttt caaagctttc ttgtcaaaaa aggctgtccc gtatacacgt tgcatcattt
     4981 gattgtttga gtttccacgc caataagcac ctgccactga taataattta aatacttgta
     5041 tacggccagt ggatggaaca tgtggtccac gacacaaatc gatgaattcg ccttgttgat
     5101 aagcagtaat cacttcttct tctggcaatt cactaatcag ttctactttg tacggatcac
     5161 ttgcaaaaat ttctaatgct tcctgttttg aaagaacacg gcgttcgatc ggcagatttt
     5221 ctttgacgat tttcatcatt tctgcttcga ttgccggcaa atcttctgca gttacttggt
     5281 tttgtccatt atctgtatcg tagtaaaacc ctgaatcgat agctggtcca acgccaaagt
     5341 ggatatttgg atagaggcga cgcatggctt gggccatcaa atgagctgct gaatgacgga
     5401 ctaagccaag tgcatcttca tggtctggtg tgatgatctc aatacttgca tcttcctcga
     5461 ttggacgagt caaatcaacg agttttccgt tcactttacc agctaaagct ttttttgcta
     5521 agctttttga aatgctttca gcaattgcca atgttgttgt tccagactca aattctttga
     5581 ctgcaccatc tggaaaagta atttttatca taatagtttc ctccttgaaa tgttttgatt
     5641 gatcagatca tcaagctgaa aaacaaaaaa atcctagcat ctcttgaaaa gagatactag
     5701 gacgaaaagt tcgtggttcc acctaatgtt caaacgcaaa tgcgttcctc aagcgttgta
     5761 acggtccgac cgcccttgct tcaacaaggg tttcgaaagt ggtcatcctt tggttttctc
     5821 ctggaaaagt ttcagcctag cttttcctct cttatcgaag actctcaaat tcagttggct
     5881 ttctcatcaa tcaattatgt gtttatttaa ccactaataa atagaaaaat caagtgggaa
     5941 actaaactta ttttttccat tccccattta caccgtatgt tccttttttc atctcattga
     6001 tgatgacatg gatgtgttct tttggtgcct gcgtgttttt atgaacagct tccgtgatat
     6061 ctttcatcat attttctaat tgttccggac tgcgaccttc tactaattcg acatgtacga
     6121 atggcataat gctctctcct ctttctgatc gttctttttt caactcgatt ctatcatact
     6181 atgtttgtct tgggtactga atcaggaaaa atggctggat ctttttttgc tctcaaggca
     6241 ctcaaaatag agaccgtatc cacgacttct tgcaaaacag ctccgaataa tgcggggatt
     6301 agtcccgtac ttgcaattaa catcaaaaaa atacaaataa agatgccaat cagtacagac
     6361 tgtttcgcta ttctcatcgt atctcttgaa atcactactg cttttgctac acgcaagaga
     6421 tcatctttca agatgaccgc atctgcactt tcactagcag ctgtagaacc atgagccccc
     6481 attgcaatac cgatatctgc agcagcaagt gctggtgcgt cattcacgcc gtctcctacc
     6541 atcgcaacag gtctttgatc tttagggata ctttctaaaa tggtcagttt atcttgaggc
     6601 aagctacggg catgtacaga atctaattgg actttttctg caatcgtttg agcaatggca
     6661 gatccatccc cggtgatcat catcaaattt ttcacatcaa gttttcttag ctccttgatt
     6721 gtctttgctg cttctgggcg aatctgatcc gaaaatagga tcgttcctgc aaaagatcga
     6781 tcaattgtta catagattgc tgtattggac gtttctattt cttcagctga aacaaaagag
     6841 gcacgtccaa tctttacttc atgattattg actactgctt ggattccttg accggtcact
     6901 tctttcagtt gactaacagg atacagttgg ctgtttttca cgttgttcac taacgaacga
     6961 gctaagatat ggctagattc ctgttctgca ctagctgcat acagaagtag ctcttctttt
     7021 tttattccat tgactggctg aatcgcctct acttccaata tccctttggt aagcgttcct
     7081 gttttatcaa aagcaatcgt ttttgtacgt gcgagctttt caatcgttgt tccagttttt
     7141 acaacgatcc cgtttttact tgaacggctc attcctgcaa ctaaagcaat gggcgcagct
     7201 agaatcagtg gacaaggtga tgccactacc agtacctcag caaatcgtaa agggtctttt
     7261 gagaaccacc aagaaatacc accgatcaaa taagcaatca acgtgaaagg gactgcataa
     7321 cgatcagcta aacggacaaa tcgggcaggc ttttcttttg attcttcgac tagtttaata
     7381 atcatttgat actggctgtc tgcggcacgt ttttcagcga tcatttggat cgatccgtct
     7441 ccatttaaag accctgacat aatctgatct ccaggtttct tggtttccgg gcgggactca
     7501 cctgtcaaag aagattcatc gaccgaagat tctcctttgg tcactcgacc atctgcgggt
     7561 acaagttcgc caggcttgac tagtaactgg tcatcaatat ttatttcatc gattactata
     7621 tcctgcaatt ttccttcttt gaatcgatgg gcaattcttg gggtgttatc taacaaagac
     7681 tgcagttctt tacccgctcg tttattcgcg taatcctcta gactatctcc gcccgtaagc
     7741 atgatcaata tcatcaaact agcccagtat tctttgacgg ctaatgttgc aaggattgcg
     7801 gtaatcgcta atacatctac gccatatctt cctgaacgta acgtcttgat catttccaaa
     7861 aacatcaaga aggcgagaat ccctccagta aatgcgacaa tcgcaaatgc aagggccggc
     7921 tggtgaaaaa caaattcact taataaagct atcccaccaa taagcaaagt aagtaaaaat
     7981 tttttgaaat gtgtcattgt tttttctcct cttttatgtg acttagagag gttagtaata
     8041 aatttccagc tgcctctttc atcttaagca tagtccttga ctagcacttt ttaaaacaat
     8101 aggccttaat tgaaaacaac tatcactatc agcgtttcca aaattcatca gttctacaaa
     8161 caaaaatatt cagcaaggat tactttttcg attcatcagt taaaaagtac cttttttcga
     8221 aaaactatgc tatactttta attcgttatt tacggcggaa ggagcaaaca tgcgatgaat
     8281 gagaaacaac tagaacaaac gcacgtggat gaaacgatcg aactaatcca gatcgaacaa
     8341 aatatgctgg atacaaaaca acaagaattg actcaggaaa tgaatcattc gacaaaagac
     8401 acagctaatc acaagattcg agctggttcc aatgaagctt tttatgaatc tgctgttgaa
     8461 tatcgccaac atgagcagga attgcttttg aaataccata ctcttgaagc acagcaaaaa
     8521 cgcctgcaaa cgttgtctgt catgaaagat agtccttatt ttgcccgtat cgactttaaa
     8581 gaagaccgtg aaacagaaac attatatcta ggtatcgctt ctttaagaga tctggcagaa
     8641 gaaacaatcg ttattgactg gcgcgcacca attgccaatc tttattatga aggtgagatt
     8701 ggtcccgcat tttatgaaac tgaaacagat agaatagatg tcgatctgca gttgaaacga
     8761 caatttaaaa tccaagaagg acgtatttta tctatggtag atacgtcgga agtaatcaat
     8821 gatgactttt tgctagagat attggatgaa tcatcaagca accatatgaa aaacatcgtc
     8881 tctacgatac aaaaagcaca aaatgcaatc attcgggaca caacaagtaa agtaatgctg
     8941 attgaaggaa tcgccggaag tggaaagaca tccgctttgc ttcaacgtat tgcttttctg
     9001 ttgtatcata atcgcaagtg gctcgaggct gaaaacgttc ttctattttc acccaaccat
     9061 ttgttttctg attacatctc gactgtattg ccttcattag gtgaaagcgg cgtgcctaca
     9121 cagacattca aaaattattt ggtgcagctg ctgccagagt tttctttact agatgaacag
     9181 caacaagaaa gtggtttttt gctaggggaa aaagatccga tacaagtgat gaaaagcgga
     9241 ttgaccttag tcgatcagat tgatcgctat atccaaggca tcacaaacta cggtcccttg
     9301 ttccgtgata tgaaaataaa tggccgtacg atcctatcaa aggaatcgat tcgccaatgg
     9361 tataaagaaa caaatgaaca attaccttta catcatagac tttctctatt acaaacgaag
     9421 ctcctgaaaa aattaggcgg tctgcaaaaa gacgaaacac gtcagcaatg ggtcaaagat
     9481 ctggcggaag aacagctcca agaactatac gcaacagatc caaatcttga atatacagaa
     9541 caaaaggagc gttcattacg taaaaaactg gccaaccaaa tcgtacgtaa aagattccgt
     9601 cgaatccatc gagggatcat gaattatcaa tttgtcaata taacgaaaca atatctccac
     9661 tttctacaaa ctgtgccact ttctcttcta acacgacata aaatcagcga gaccaggtgg
     9721 caggaacatt tgacaactgt tcgtacacac ttgcgtcaaa gagaattgcg tcaagaggat
     9781 gctgtactat tctttttact gatgcgccga acatatccag tgacggttac acacaaagct
     9841 cgctatatct ttattgatga aatgcaggat ttcgcaccat cacaagtggc attgctgcaa
     9901 gccttgttcc caaaagcaaa tttgactttc tgtggagatc taaatcaaaa tgtctttgga
     9961 aatgaaacga tcgttggatc attggaagac cttttcgtgg accgtgaagt cactcgtttc
    10021 caattaacga caagctatcg ctccacaaaa gaaatcactg attttgctaa tcattttatt
    10081 gtgaaagaac atcaagtaga aacaacagca agaaaaggag accttccttt gctcatccaa
    10141 ggaacaacag atgatatcaa attgaattgg ttagcagata ccattcgaac gactcaggga
    10201 aaagcccggt attggcgtac agcaatcata gctaaaacac aagaagaatg cgaagctctt
    10261 tatgctcaac tgccagacga gttgaaacaa caagtccagc tgattttaaa tgagagtgac
    10321 tttatgaagc gttccattat tttacttcca gcctttttag caaaaggtct ggagtttgac
    10381 cgagttttct tgtgggatgt agatgcagaa catttttctt ctcagcaaga caaattgatc
    10441 ctttacacca tgtgtacacg tgcgatgcat gaattgaccc tttttacctc caataatgaa
    10501 agtcctttgt taaacaatat ggatatttca tcttatgaga aaaaagtact gtaagtaaaa
    10561 agacaactgc tatcattaat gttagcagtt gtctttttat ttacaataat aattgcagaa
    10621 aaagtaattc aatcatttaa ggtcatttat tctatatata aatccaaatc tgtttcagga
    10681 tattttaatt tgacaagcga aataatcatt ttcagttctt ccatgttttc tggatattct
    10741 gggtcaaata ccttgctatg acggccaatc ccgccattgt cgatcaatcg gtaaattcga
    10801 ttccattcaa tgaattggtc aattgattgg aaattgctga ttggatagcc gccacaatcc
    10861 aaaatctctg atacacgaaa tgctaggtat gcactatcga tcactttatt ttttttcgtg
    10921 tctatcttct catttctttc gataagaatt ttttcaggac gatgtttcaa gctccactct
    10981 aatagtagaa agaaagcctc catccgatac tcttcttcaa ataaccgaaa ggctgaatca
    11041 gaacgacgaa aaagcagcgg aatcagataa ttttgtctat aaatcagcat tggtgcgcgg
    11101 tccaccatac actctaaata acggttcaga taccctgtac cgacatagtt tcctaatcga
    11161 tagccttcaa cgccgaatct tgcgtatttt tgcgctaatt ttgaacaatc agttacgttc
    11221 acagctaact cttctacttt gcggtaaccg cttaagatac gttcaaaagg ttctaaagtg
    11281 atttttgctt ccatattctt ccccgtccat cttccacata cttcatatca tttcacatga
    11341 tacttatgct ctcatcatac cacaggttga attagaaaag aaaataaatc acacaaaaaa
    11401 gcaagacaat ctgttagtga gattgtcttg cttttcgaaa aagactgaat acttttcgtt
    11461 aaaactggtt gcttagtctt ctaatgccac attatggaac acatgctgta catcttctag
    11521 atcttccagt gcatcaatca ttttttcaaa tgtttcttga tcttctggag atagtgttac
    11581 ctcgttttga gggatcattt ggatctcagc gattgaaaaa tcagtgattc ctttttcttt
    11641 taatgcttct tgtatactat ggaaatccgc tacttcaccg taaacgatga tttgtccttc
    11701 ttcttccatt acatcgcgaa catcgatgtc tttttccatt aggtattcaa ggatctcgtc
    11761 agcgtcttct cctgcaaaag caaagattgc tgtattgtca aacatgtaag aaaccgctcc
    11821 ggaaacaccc atgttcccgc catttttccc aaatgctgcc cgtacgtctg ctgctgttcg
    11881 attcacattg ttcgtcaatg tatcaacaat caccattgag ccgcttggtc caaatccttc
    11941 atagcgcaat tcagagtatg tttcatctcc tgcacctttt gctttttcga ttgctcggtc
    12001 aataatatgt tttggtacat tataagtttt agcgcgatca atgacgaaac gtagtttttg
    12061 gtttgcatgc ggatcaggat cacctgattt tgctgccgcg tagatttcaa tcccaaattt
    12121 tgcataaatc cggctattat tagcatcttt agcagttttc tttgctacga tatttgccca
    12181 tttacgtccc atattttcac ttcgctttct gatttgatag tttcataacg caaaaacgtc
    12241 ctgttccatt atacttgtga atcacgattc tgtgaagctt tttttctttc ttccttcatt
    12301 cttctcattt ttagtcaatt atctatactc ggattgggat ttgcccgaaa ttctgttccg
    12361 gaatattgcc tcccaattga tagatttgat cacttctaac taacagttcc gcttcttgtg
    12421 cctctttttt tccgattcgg gaataaatag aaatctcttt tttttctttt aagaatcttc
    12481 ttccagcatc ggtaaatcca agtagtcgaa gataattttt attttgttgg acaggaatct
    12541 cttgcttttt gatattcagc aagacataac aagataatcg ttggatccgt gtccatgtat
    12601 atctttttgt ttttaccttt gacacaaaat cctcaaaact atcagcagtt gcagctgctg
    12661 ttttcatgcg cgattctatt ccttcggtca tctgatagat atctgtcagt tccagcaaag
    12721 tagaagataa caaccgataa cgcaaatacg gccaataaga ttcccaagtc tgcagcgaac
    12781 aagttgaaag atcttcttct gtctgaggcg gcacaagtcc agctattctt tttccttctt
    12841 ctaacgcatg gcgaatggct gttgcactgg ctatcctctg atctgtgatg attttttcat
    12901 gatagcctgc acttttacgt cggatcggat gtaattccat agggtggaca tacttggcat
    12961 tttcctttgc ataacttaat cccaagatat gatttggcga agaaaaatca agcgtaatat
    13021 cgggatacag actgcgaaaa acctcagtca ttttttgcgg ataactatac gtttgatcag
    13081 acagttcttg aaatgcctct tctatcctat cttgattctc ttggacaaag cgtccgaaac
    13141 tgctgtaatc aaacgaagtc tcttcttctg ttccaaaaca caaactgtca cagtccagtg
    13201 tttgcaagat ttttactgca cctttagcaa aatagtcagc tgactgaacc gcccattcga
    13261 cgggaagctc cactactaaa tccacaccat tttgtaaagc ttcattcgcg cgatgccatt
    13321 tatcgatgat agccggttcg cctctttgca aaaaattccc gctcattaca gcaatgacga
    13381 cctctgctcc tgttattttt cgtgccattt ccacatgata gcgatgtcca ttgtgaaaag
    13441 gattgtactc tacgataatc ccgcagcttt tcatgcgcat cctccttcat agttatcgat
    13501 aaaaaagaaa tagagacaag taaaaaaagg caaaaatcgg aaaaaatacc cgaactatat
    13561 aaactattct cgcaaacaca atacttctgc gacgaaaagc tgcactgcaa gagcaatgcc
    13621 tatatcgttc ggatatataa tttatgttga agatttattc ttttcttgtt aactcgagca
    13681 ggcaaaaccg ctagtctttg tatgctacaa agaaccagcg ctttgatttc tctgatgctg
    13741 cttcgtctgt aaaatcagaa aatgcttcga ctttcgaaaa gccgctgttt tccagcattc
    13801 ttaaataact ttccaatgga taggtacgct cttgatgtag ttcatcttca cgaataaatc
    13861 gaccagaccc ttcttgttct tcaacgaaaa aggtaagaaa atgttcgatg ctgtgtttct
    13921 cgcttcctgg ataactatcc cacaaaaaag caaaattatc cccttgataa tgatagctat
    13981 attctggaaa aacctcatct acttgatagg tggagtgtac gtcaaataaa aatcggccat
    14041 tttcttctaa cgcttgatag acttcatcaa acacttgctg tacctcttgt ctatttttca
    14101 tatagcacag cgaatcagaa aaacaggtaa ttgcctgata ttgtccgatt tctgataaat
    14161 cgagcatatt gccttccaca aattggatcg atgcgtcttc ttcatatgct cgctggctgg
    14221 caatcatcaa catctcttct gaaaggtcta accctgtcac tttatatcca tcttttgcaa
    14281 aggcaaccgc taatgctcca gtaccgcatg ccagttccaa aatatttttt gtgtcttctt
    14341 gtaaatgacg tttcgaaaaa gctaaccatt gctgataaag tgtttcgtcc atcacttcat
    14401 cgtaaatgaa agcaaatgtt tcataagcca tatgttaatc caaccattca ctaagatcga
    14461 ctagcggagc atctgaccat agtttttcca gattatagaa atttcgttct ggcgcatgga
    14521 aaacatgtac gatcacatct ccaagatcga tcaataccca ttttccgcct tcttttcctt
    14581 caatacgttt tacttcatat ttgttttctt cttctttatc aatgatttct tctgtaattg
    14641 cgttgatttg acgttcgcta tttgctgaac agatcatgaa ataatctgcc aacaaggaaa
    14701 catttcgaac gtctaaagcg ataatgtctt ctgcccgttt tgaatctgct gcttcaatcg
    14761 caatttttaa ttgttcttta ctatctatga tgatttcctc ctaatatgag cagcttattt
    14821 ttttgctacc cagtaattat atgtttcgat tgtttttgga taaatctgtt gttcttgcgc
    14881 aagcaaatgg gccaatgtat gttttgtttc aaatgctaca gcttcgtcta gatcagccca
    14941 tgcaatggct cgtgcatctt gtaccccagg aaaatctcga cctggttcaa tatagtctgc
    15001 tacataaatg attttgtcaa ggagactcat cttagcagcc ccagtcgtat gcacccggat
    15061 tgcatgaaga atctctgcat cggtgattcc taattcccgc tctacaaacg aagcgccaac
    15121 aagaccatgc cagattgcat tattataatt caataactcc ggatcaaaac cgtctcttac
    15181 gattaccatt ttgaactcct catctggtcg ttcttttgca taatcatgtg tcaatgcagc
    15241 aatactagct ttttcaggag aagcaccgta ttttttggca agagccacgg ctgtttcttc
    15301 tactccaagc acatgcttga agcgttgttc gctcattctc atctgtattt tttgcatcag
    15361 gttttctcta gaataagcag tatattcatt actatagtcc atattcatac aacccttcat
    15421 tttgaatata atcaattact ttatctggaa caaggtagcg gatagaacag ccttctttga
    15481 tcttttgacg tattttcgtg gaactgatgt cgatctcagg tacatctacc caaattacag
    15541 gatatggagt atccgttgtg tatcctggtc gacgtatacc cacaaagttg accatagaag
    15601 tcagctcatc gattttatac cattttggca aatattctac catatcccca ccgataataa
    15661 agtaataatc tgtatccgga ttgttttgcg tcaattcctt catagtatca taggtgtaac
    15721 ttttcccacc acgagctaat tcaatcgttt caatctgcaa aaaaggattg tcttccaccg
    15781 ctaattccaa catgttgagt cgatgttttg catcaatcgt ctgtttctca tcaacatgag
    15841 gtggctgata agaaggcatc aaaaatacgc ggtcaagacc taaattccgg ccagcttgtt
    15901 cagccatcac taaatgtgca aggtgaacgg gattgaatgt tccgcccaag atccctactt
    15961 gctttttctt ctctaagaat tgaggcattt cttcaggaaa taattgtgaa cgaacaaatg
    16021 tcttagcttg cgtattcatc atggtcactc cttatattgc ttttacttct tttgatagcc
    16081 tttgatactt ttcttttgtg gaaggtttgt ataatacaat aacacgtcca atgatttgga
    16141 cgatttcgca atctatttct ttttctaata cttgagctgc ttcttctgca atctcatctg
    16201 tattttgaag taacgatact ttgatcagtt ctcttttttc taaagcttct tcaatttgaa
    16261 cgatcattgc tgaattgatc ccaccttttc cgatttgaaa aatcggctga agatgatgcg
    16321 ctttgctacg taaaaaacgc ttttgttttc ctcttaaact cataattttc cttcttcctt
    16381 aaatcaatgc tttgcgtttc aatacatcta caccctttgg cgcccagccc gcaattacta
    16441 ctggcgctgt tacagtgatc cagcctagac cggcaaaaac gatgtctgtt ttttctttca
    16501 cagaaaactc aaaacgaaca agctccggaa atgcctcgac ttcatcagga cgaggaggct
    16561 gtaataatcc acctacatgt ttttgataaa attcgtctgc tttttccagt ttcgttcgat
    16621 gaatatttac atcatttgac acataagcaa taaatgaagc acgattgccg ctgataaaat
    16681 caaatcttgc aagtccgccc aagaaaagtg tttgcccttc attcagctga taaactttag
    16741 gtttgatttc ttttaacggt gcaatgatcc gcaaatcttt tttacctaag tagtgcgcca
    16801 tttgatggcg atggataatt cccggtgtat caatcaaaaa atgcccgtca tctaaaggaa
    16861 tctcgatttt atccaaggtc gttcctggaa actgcgaagt tgtgattaca tcttgtactc
    16921 ccgctgtatt tttgatgatt tgattgataa gcgtcgattt tccgacattt gttactccga
    16981 cgatatagac atcccgtcca tcgcggtatt cttcgatttt ttctaatagt tccgtcatct
    17041 catgagattt cttagcacta gttaataata catctactgg acgaattcct gcttcgtaag
    17101 cacgttcctt catccattgt gttaatttag tttttttcaa tgatttaggt aatatatcca
    17161 ctttgttccc aactaaaagt actggattgt caccgacaaa acggtgtagt cctgggatta
    17221 gtgaaccatt gaagtcaaaa atatccacca cattgacgat caatgcatct ttttgcccta
    17281 attcattcag taagcgcaaa aagtcgtcat ccgtcaatga gacatcttgg gtctcattat
    17341 aatggcgtaa tctgaaacaa cgctgacaat agacttcccc ggaagctaaa cctttttcca
    17401 aagccgtttg cggcgtgtat cctaattcac cgggtttgtt tgtttgtata acggcaccac
    17461 agccgataca ttgtaattct tctgtcattc gattccacct cgccagccca tatcagggtg
    17521 ttttgctaat agataggcca taattctttt ttcccaaaaa cgattaaatt gtgtcttcca
    17581 actatctgtt gcgataatgg gttttacaag gatgctacgt atccctgcac gattggctcc
    17641 acgtatatca gtcataatct gatcgcctac cattactatc tcatcatctg ctaaatccaa
    17701 ttgtttcttt gctatgttga tccctcttgc taaaggtttc attgctcgtg caacatacaa
    17761 aagatcaaat ttttcaatcg ctcgcgcgac acgttttgaa ttattgtttg aaacaacaac
    17821 aacagggatt cctgcatttt tcatttctaa aatccaagta agcaactctt ctgttccatc
    17881 tggattattc catgcaatca acgtattgtc taaatcggtc aatactgctt tgatgcctag
    17941 attctttaat tgggcaggtg taatctgata gatggcttct accatccatg ttggtttgta
    18001 atttgaaaac atgctgtctc ctcatctgca aaaaatgaag gagcacataa agtactcctc
    18061 aaccccgtat tatactgcat atcagcaaaa aaaactacct ctatactcgt tcgtctttca
    18121 gaaaaagcat ttctttacaa aatgaccaaa aataaagatt gatcacagaa aaatttagaa
    18181 acaaaaaaca actaatattg gcggaacctc ttgtatcgtt tttccagtaa tttatgctga
    18241 tctaatcgaa caagttccgt caatctttca atcaacgctt ttttcataat gtgtgcgact
    18301 ttatatcgtg gcaactgctc gccgttgaac gtttcaggaa taactcgatc aacgattcct
    18361 aactcgcgta gttctggcgc agtaattttc atcaactcag ctgcttctgc ggcgcgacta
    18421 ccgtctttcc acaaaatcga cgcaaatcct tctggagaaa gcacagcata aatcgtttct
    18481 tccagcatcc agacttcatc cgctaaacct agagcaaggg caccgccact tccaccttca
    18541 ccaatgatga tggcgatgat cggtaccttt aaatcagaca tttcgagcag atttctggca
    18601 atagcctctc cttcgccgcg ctcttctgct cctacaccgc aatatgcacc tgctgtattg
    18661 atcaaggtaa tcaccggtct attgaacttt tccgcttgtt tcatcagtcg taaagctttt
    18721 cgatagcctt caggatgggg agaaccaaaa tttctgcgga tattttcctg cagatcccgt
    18781 cctttttgaa taccgatcac tgttaccggt ttgccatcta atcgagcgat tccaccaaca
    18841 atcgcttcgt catctgcaaa taaacgatca ccatggcatt cctcgaaaga atcgaagatc
    18901 tcttcaaaaa aatccaagct tgtccatcgt tctttagctc ttgctaactg aacgacatca
    18961 tgtgctgtct tatccacgat ctatccatcc tttctgtgta tgcaattcaa gaagcgtatg
    19021 gattttctgt ttcaattcca tgcggggaac aatctgatcg acaaaaccat gagataataa
    19081 gaattcagcc ttttggaaat cttctggaag ttcctgcttg atcgtctgtt cgatgacgcg
    19141 tcttccagca aaaccgatca agctttgggg ttctgctaga ataatatcgc cttccattgc
    19201 aaaactggca gttactcctc cggtcgtcgg gtccgttaaa acagttagat aaaaaagacc
    19261 ttcattgcta tgccgtttga cagcagcaga gatttttgcc atctgcatta gagaaaagat
    19321 tccttcttgc atccgtgcgc cacctgaagc tgtgaacaag acgactggaa gactttcctt
    19381 tgttgctcgt tcgaataatc tggtgatctt ttctccgaca atcgttccca tacttcccat
    19441 gataaaatta ggatccatca ctccaataat gaagggaatc ccctgaatgg ttgctttacc
    19501 agtgaggact gcttcatgaa gtcctgtttt atcttgcatt tttttaattt tttccgtata
    19561 ttcgggaaat gctagcggat ctttggttac aagatccgta tcccattctt caaaactttt
    19621 ttcatcaata atcaaagcta gacgctgcca tgcaccgatt cgaaaagcgt atccgcaatg
    19681 agggcatact ttttcttcac cgatgtcttt cgtataaagg atatgtttgc aatttggaca
    19741 ctttgcccac atgttatcag gaacagatgg cgagttagca ggtttgctct ctctattagg
    19801 attgatccga atatatttct ttttcttaaa cagagccatc tatgcgcctc ctatctccgt
    19861 ttctggtgtc caattcggta aaaattcttc ttgtaaaaat gctgtgctgt aatcacctgc
    19921 tatgactgca ggatgactaa tcaaatccat ctgaaattct gcgttcgtga tgattccttc
    19981 tgtgataaat tcacttaagg ctcgctgcat cttcattaat gcttcaaaac gcgtatttcc
    20041 atggacgatt atttttgcaa tcattgaatc ataatatggc gggatcgtat atcccgggta
    20101 aactgcactg tctactcgca gtcccatccc acctgctgga agcagtaagt tttggatctt
    20161 ccctggagaa ggagcaaaat ggaaagcagg attttctgca ttaatccggc attcgattgc
    20221 gtgtccttgg aatactactt cttcttgaca cacattcaaa ggctcaccgg ctgctatctc
    20281 cacctgtttt tttactaaat cgattcctgt gaccatttct gttactggat gttccacttg
    20341 gattcgagta ttcatttcca tgaaataaaa atcgccttct tcatccatca gaaactcgat
    20401 cgttccggca ttttcataat tgactgcttt agctgctcga acagcagcgt ctcctagtgc
    20461 ttctcttttt gtttgtgaga tcactacaga aggagattct tccagtactt tttgattgtt
    20521 gcgttgtaaa gaacaatctc gctcgcctaa atgaatgaca tgaccaaatc gatcacctaa
    20581 gatctgaact tctatatggc gagcaggata aatgattttt tccaaataca tatcatcatt
    20641 tccaaatgcc gctgctgctt cttgctgagc tgaagaaaaa tgctgtggga gctcttcttt
    20701 tgattgaact ttccgaatgc ctttcccgcc accacctgca gctgctttca gcatcactgg
    20761 ataaccaatt ctgtctgcta tttccaatgc ttcttctact gtagataata caccagtact
    20821 tcctggaatg actgggacat ttgcttcttg catcaattga cgcgcattga tcttatttcc
    20881 cattgcatcg atcgttgcac ttttaggacc aataaatgtg atattgcact cttcacacat
    20941 ggaggcaaat cggctatttt cagataaaaa accaaatcct ggatgaattg cttcggcttt
    21001 tgtcacgatt gcagcgctca gtacttcctg aacattcaga tatgaatcaa cggcttttgc
    21061 cgggccaatg cagattgctt catctgctaa ctgtgtatga agtgcttcgg catctgcttc
    21121 tgaatatatc gctacggttt ggatccctag ttcgcggcat gcacgaatga tacgtacagc
    21181 gatttcaccg cggttagcta tcaatacttt agaaaacata tttcacctat ccaatcataa
    21241 aggttaattc cgcttcggct acttttttac cgtctacttt agcgattcct ttgcctattc
    21301 cggctgaagc tttcactttc aatatctcaa cttcaaggta taaggtatct cccggtgtaa
    21361 ctttcttacg gaattttgct ttgtccaatc cgccgaaata agccgtcttt cccttgaatt
    21421 gttcaagaga aagtaatgca actgctcctg cttgagccat tgcttcaaca atcaataccc
    21481 ctggcataac aggttcttct ggaaaatgtc cttgaaaaaa gggctcattg atcgttacat
    21541 ttttcttagc aacgatccgt tctccttcaa ccatttcttc cactcgatca atcaaaagca
    21601 ttggataacg atgtggaatg atttctttta tttcttgtat gttcattgtt ttccctcttt
    21661 cctgctttgc ttatttttgc tgaatcctaa agagtggctg tccatattcg acaactgact
    21721 cgttttctac aaggatttct gtgatcactc cgtcggcctc actagttatc tcattcatca
    21781 gtttcatcgc ttcaataatg cagataactt ctcctttggc aacttgatca cctacttttt
    21841 tgaaagctgg tttgtcgggc gctggttgca gataaacgat tcccacgatt ggcgaaacaa
    21901 cctcgattcc atcaaatact gccgattctt tttctttcat tatttctgga gcagttgttg
    21961 cttgttcagg tatgacattg actggctccg aaactggcaa cgtttcgttt gtttgaacca
    22021 tattcatgcc tcgacttgtt ttatttttat tcatatacaa ttcaaattga ccttcacgca
    22081 aatcgaattc cgttagcgag gattgatcaa attgactgac taattctctg atttcattga
    22141 tatccaaaat tattcctccc agcgtttcat gcacagaacc gcattatgtc cgccaaaccc
    22201 taatgaatta ctcattgcat aagtaaaact tgcaggtttg ctttcgtttt tgacaactgt
    22261 caagtcaatc tcaggatctt gtttttctac attgatcgta ggtgggataa attgatgttc
    22321 taatgccagc aaagttgcca aggcttcgat cccgccggct gctcccaata aatgtccagt
    22381 catcgatttc gtgctgctga cgtaagtatc tctgaaattt tcacctaatg cgatttggat
    22441 tgcttttgct tctgcctggt cattggctgg tgtacttgtt ccgtgagcat tgatatatcc
    22501 gacttgttcc ggctgtacac cagcttcttc catcgcaagc ttcattgctt tagatgcacc
    22561 agaaccatct ggagtaggag ctgtcatatg gtaagcgtca cagtttgcac cgtatccaac
    22621 gatttctcca tagattttgg caccgcgagc ttgtgcatgt tctaaagatt caagaactag
    22681 aacgcctgct ccttcaccca tcacaaaacc attccgattt tcgtcaaacg gaatcgaacc
    22741 acgattagga tcagttgatt ctgagagtgc tgttaaagct gcaaatccag caatccccat
    22801 ctcacaaatc gttgcttctg ttcctccagc aagaatgaca tcagaatagc catgtttgat
    22861 gttacggaat gcttccccaa ttgaattcgt tccgctagca caagctgtta cgatagctgt
    22921 acaaatacct tttgcaccta cacgcaaagc aatatttcca gctgccatgt tggcaatcgc
    22981 cattggtacg aacattggag aaactcgttt tgcacctttt tcatgtagac gaataacttg
    23041 gttttcgatt gttggaagac caccgatccc agatccgatg atgacgccaa accggtcttg
    23101 attcattttt tctttatcta aaccgctcat ctccattgct tctaaagcag cgtagattcc
    23161 atagatcgaa aacatatcca ttcgtttgct atctttttta ataaaatatt tatcgtaagg
    23221 gaaatctttg acttcacccg ctaaggtaat ccctgtttct gaagcatcaa atttagtgat
    23281 cggtgcgatg ccattcgtcc cctcttttaa gctattcaaa aagctgtctg cgtcatttcc
    23341 aataggtgaa gtgactccat agccagtaat taccactcga ttcatttgtt tgctctcctt
    23401 ttcttatccg tgcatgacca atccgccatc gacattgatg acttgtccgg tgatatatgg
    23461 attttgtgct aagaaaactg ctgtttgtgc aatatcttct acttgtccga aacgctgcat
    23521 cggaatttgc ttttccgctt gttccttgac tttgtctgct aaaacttccg tcatatcagt
    23581 tgtgatgaat cctggcgcaa tggcattaca tgtaatgcct ctagttgcag cttctcgtgc
    23641 cacagatttt gttaatccta ctacgccagc tttactagct gcataatttg cttgaccaat
    23701 atttccaatc aacccagata cactagagag attaatgatg gccccttctc gctgcttcat
    23761 catttttttc aatacgtgct gcgtcatatt gaaggttcca atcaaattga tatccagaca
    23821 ttttttgaaa tcttctgcat ccattcgcat cacaagtttg tcatttgtga ttccggcatt
    23881 gtttactaac acatggatcg atccaagctt ttcttctgct tctttgatca tttgtcccgc
    23941 tttctcataa tcagagatgt caccggaaac tcctacgcat tttacaccga atgcttcgat
    24001 ttcttctatc ttttcttttg gaatctctcc gcgaccattc aaaatgatat ttgctccagc
    24061 ttttgcaaaa gctaaggcca tcgctttgcc gattcctctt gtactacctg tgataaatac
    24121 attttttcct tttacatcca taacgttcct ccttatagta ttgctattgt ttcatcaaat
    24181 gtctgcttat tctcaacacg cagaactggt attgttttat cgattttttt cataaatccg
    24241 cttaatactt ttccggggcc gacttcgatg acttgttcga tgccgattgt ttttaactta
    24301 tggatactct catagaaacg aacaggttgc atgacttgct gttcaagtag tcccgcgatt
    24361 gtttctttct tcattatttc cgttgttgtg ttgcttatga taggaaaaga aggttcagag
    24421 aattgaatct gacttaggtc cttcgccagt ttttttgctg ccggttctaa aatagctgta
    24481 tgaaaaggcc cgctgacatt tagagggatc attcttttga accctttttc ttttaatagt
    24541 aaaacagctt cgtctactgc tttttcttcg ccaccaatca cgatctgctg aggtgtatta
    24601 taatttgcag gagatacgat gccgtacttg ctagcttcat gacagctttc ctcgatcgtt
    24661 tcaatcggcg cattcatcac tgcaaccatt ttcccgcttc cagctggggc agcttctgtc
    24721 atataagcgc ctctttttgc tactaacgca actgcttcag tgaaggatag cgcattacta
    24781 gctactaatg ccgaatattc tcctaaactc agtcctaaag ctgcatctgg gatgattcca
    24841 tgctccttca ataagcgata atacgcgatg ctgactgtta gaatagctgg ctgtgtatat
    24901 tgcgtctgat ccaaacgttc attttctgta aagcaaagtt cagccatatc gtagccgaga
    24961 atctcacttg cttcatcaaa tgtctgcttt acaacggctt cttcatataa atcttttccc
    25021 ataccttgat attgtgcacc ttggccacta aataagaatg ctgttttcat gtttttctcc
    25081 aatatatcca gaatatatgt ttgattcttc ctattcaaaa aggccgagac attcctattt
    25141 caggaaatgt cccagccccc aacctaattt taatgattat gcagtttgtt tttctacgta
    25201 agtcactaaa tcttgaaccg ttttgattcc gtcttctgtt tcgattttca cgtcaaattc
    25261 atcttcaatt tcattgatga tttggaataa atccaagctg tcagcttcta agtcttcttg
    25321 gatgtcagta gtcaatttga tttcttcagt ttcttttcct aattcttctg cgatgatttc
    25381 ttgaattttt tcgaatacca taataatata ttcctccgat attgtttgct ttttattttt
    25441 ataatttaac agtctacaac tccatgagaa tacttcccca tgttaaacct ccgccatagc
    25501 ctgtaaatat tactttctgc tgttttccta aggtgatctt tccactagaa actgcttcat
    25561 ccaataagat agggatgctt gcagcagatg tattcccata tttatccata ttggtcagga
    25621 acttttcttg cggcatcttt acttttcggg caattttctc aataattctg aggtttgcct
    25681 gatgcaaaag cagataatca actggtttgt ctttcatgac ttcttggata ttgtttgcaa
    25741 cgtctcgtac ggcaaaatca aaaatatctc tgccagtcat ttgcaaataa tacgaacctt
    25801 ctgaatcttc tgaatagaac ggatttttat tttgctggta gccagcggtc aatgagctac
    25861 ttctggtacc gtcagctgct aatttttctt tcagcaattt tttctgtgga ttcgcttcta
    25921 agagaatccc acctgctcca tcgccaaaca acacagcagt cgtacgatca gaccaatcga
    25981 ttagcttaga tagtgtttca ccaccaatca ccagtccctt agttgagccg cagcggatca
    26041 ttttttcagc catgcttaac gcataaacaa atcctgagca cgcagcactg atatcaaaag
    26101 cgatagcatg agttgctccc aatttccctt gtacttgtgc tgctacagat ggtgtcgtgt
    26161 aatcgggcga cattgttgct actaaaataa aatccaaatc tttagggttt agatttttct
    26221 gttttatcaa tttttccgcc actaaaatgc ataaatcaga agtctcctga tttgttacac
    26281 atcttcgttc acgaataccc gtccttgacg aaatccattc atcagaggta tccatcatct
    26341 tggaaagatc attattagta ataatctttt caggaaggac gctggctgtt gcagtaatca
    26401 ttccaaattg ttccattaaa tatcctcact tatagtcttg caagaagtcg tgcaagtttt
    26461 tcagtgcatc cagcaaggca tgctgttcct ccgtagccat tccgtctaag atgttcttga
    26521 ccatttcacg atgaaaatgc tgatggaccc gaaatagtaa ctttcctttt ttcgttagtc
    26581 ccaactttac aacgcggcgg tcatcttcac tgcggatacg ttcgacatag ccttttttta
    26641 ctaacttgtt aatagcagtg gtcaaggtcc caacagtaat tgatagttct ttggctactt
    26701 ctgaagttgt ttttttcttg tacatcccga ttgcctcgat cgtgtgcatc tctgtgattg
    26761 acagatcttt gaattgagat tttttcagct cagactcttc gattgtcaaa atatcattga
    26821 agacactaac aagataatca tttactgttt ttaaatctgg ttccatgttc caccgtccac
    26881 taattagttt gattatcaaa tcttttgatg ttcaaactat atcttaactt ttttcaaaat
    26941 gcaaccttaa aagatcattt ttttactata tgagcttttt ttaagatttt actcatttaa
    27001 aacaactgat aaatcagcat ttataatttc ttttattaga aaaatcatct atctcacata
    27061 ttttgattat caaagtattt tctgtgaaca gaactataga attccctaaa acttgtccat
    27121 tatttcaaaa tttgttatag tctttttatt caaagcgtaa aaggagatcc tatgttaaaa
    27181 gaaaaaatga aaactctccc catgctgccg ggtgtctatc ttatgaaaga ccgagatggt
    27241 aaggtcatct atgttggtaa agcaaaacgg ctcaaaaata gagtaagcag ctattttcat
    27301 cagaataaac agcattcaaa aaaagtatta cgtatgattc atcatataac agattttgat
    27361 ttcgtcgtag tagacacgga attagatgct ttattgctcg agtgtcaatt gatccaacat
    27421 taccgtcctt tttataaccg acaaatgaat tatttcagta attataatta tgtacatatt
    27481 accaataagg gatttgtatt aacagatact cctacggctc gaacatacgg gccttttcgt
    27541 ctctataaaa aaatgccgtc tattttgcgt ataatggaag aaacttatca aatgccttgg
    27601 ttgtctgaaa ttagtttgtt agctttgcga gtccagcttc ctgacctaca ggaaatgact
    27661 tttgaacaga aaaaaaagga actccaaggg ctgtttcaag gccgaaataa aaagctactg
    27721 acttacttga aaaaaagaca acaacatttc atctatcagt tgaatttcga aaaagccggt
    27781 atgcttcaaa aagatatcga attagttact tattttatta gacggatcca ggaacaaaag
    27841 cagtttttaa gaacaccatc gctcaccttc agcatgccat tagcagcaga tgagagtcag
    27901 aaaaagcact acctcgtttg ttatggtcaa cttgcagaaa caatgattac ctctggtgac
    27961 gtttctcctg acttctatta tgaaaagaaa gaagctcatt tatctttgaa acgtcaattg
    28021 tcaaaagaag aaatcgatcc tgtacaaatc ttgatcagtt accgcaaaaa attagaaaaa
    28081 gaacagtttg aaattgaact gttaaacaaa aaagaagctg aaaagcaact aaattagttg
    28141 cttctcggct tcttttaggc atcttcaact tcaaatgtca attatcctaa aatttattgg
    28201 attaagtcat agatttccat cgctacgata tcgatattat caaattcgta ttgctgatga
    28261 gtatctgttt cagttaattc aaatgtatcg gtcgctttat cataagttac tgtacacttc
    28321 tctacgcctt ctttttcaaa acggcgaact tgcagttcat tatctgctga ctcgaccata
    28381 gcctccagtc gttttatgat tgcgactaat tgtgaatgtg tcatcggagg tcctcctttt
    28441 cttatttctg ttatattgta tcaaaagcat agcctacttg cattgatttt ctctaaaaaa
    28501 actcgttttt tatgattttt tttctggtat ttttccttca ggccaccaaa gtttgatcaa
    28561 agcttgggtt ccgtcattct caaaaccttt atctgccaac tcttcatata gtttcaatgc
    28621 ttgggtagtt gctggcaata caagatccat tttttctgct tcttctaatg caatctttaa
    28681 atctttgata aaatgtttga caaaaaaacc aggtgtgtaa tcttctttta aaatacgagg
    28741 agaataattg cttaaagacc agttagctgc acttccacct gaaagcgtct caatcacttt
    28801 ctttaaatca agtccgtttt tttgtgcata gacaagcatt tccgtcattc ctgtcatcgt
    28861 acctgcaatc atgatctgat tagccatttt tgtatgctga cctttcccag cagaaccatg
    28921 caatgtaaat gtcgtaccaa attctttgaa taaaggcagt acttgatcat aaacggcttc
    28981 ttctccaccg accatgatcg tcagtgtacc attctttgct cctaagtcac caccggatac
    29041 cggtgcatct aacgcatctg cgcctttttc ttttgctgtt tttgcaattt tttctgccag
    29101 gcttggtgta cttgtagtca aatcaactaa aatatgtcct gagatatctg cagaaaaaat
    29161 ccccgactgt ccatagtaga tttcttctac atctttgggg taacctacca ttgtaaagag
    29221 aatattactt tgttctgcta ccgcttttgg tgtatctgcc cagatagctc cttctgttac
    29281 taaaggatca gcttttgctt ttgtccgatt atagacagct acttcgtgtc ctgcttctag
    29341 tagatggcgt gcgacagcac ttcccatgac gcctgttccg ataaaaccaa gtttcatttt
    29401 ttcgacctcc taaaaatcaa aaatccttgc ttctatgcta gtatacgcta gaaacaagga
    29461 ttttttcata aaaaatgttt gaattttatc ttttaaatga atttaatttt attttgattc
    29521 gttcaatcga gtcttcttct tcttttcttc gcatctcttc ttcatgataa acaaccatgt
    29581 cttgataaaa ttcgatcata gcttcgccga aatgtgtaga tgaaatatca taaagtttag
    29641 cttctaaaac ttttggatct ttaggaactt cactggaaat ataatcaata agtgctggtg
    29701 caaagtcttc gtcggtttca aaagttttcc ctaatgaagg atgatcgaac aatcggttca
    29761 aataagcatt tccttccacg acacaaggtg ttccggctgc catcgcttct gtataagtaa
    29821 gcccttgtgt ttctgaagta gaagcactga caaaataatc tgccgcttta taataaagtg
    29881 ctacatcatt gttgtccact tctcctgtga actgcacaaa ttcttctaac tgcagttctg
    29941 agatcaattc ctctaactct tctacataag gtccttttcc tacgataact aatctggcgt
    30001 taggataagc ggagatgacc tgaggaaacc cattaatcaa ggcttggata tttttctcat
    30061 aagaaatcct gcttaaagaa agaatcatca aatggttttc ctgaagacct agtgactccc
    30121 ttaatttgct gatatcctgc tgagtgatat cagaacgttt gaatttttca atgtcgatac
    30181 cagtaggaat cacgcgaagc ggtgctatta caccatagct gcgtaatgta tcaataacac
    30241 gttcacttgg acaaactaca cctgtcgtat gatttgcaaa taatctagac aagtatttta
    30301 catgggattg cctgatcact tttcctttac cgatataatg aaggtagtct tcatacatcg
    30361 tatggtaagt atggatcaga ggtattttca acttcttagc taccatcttc ccaagtatcc
    30421 ctgctccgaa ttctgtatgg gtatgaataa gatctagttc tagttctttg gcaaccaaat
    30481 aagcatacca catgcctcta acaacaactc ttcgatcctt gaaagaaata aatggtacac
    30541 ttggcattcg aatcacatct ttttccaact tatccgcatt aggatcggtc gttgtaaaaa
    30601 tatagacttc atgcccatgt ctttctaatt cttgttttaa tgttttgatc gatgtagcaa
    30661 ctccgctgac ttgcggaaag taggtatcgg taaaaaagcc gattttcatg tttttcgcct
    30721 ccaaactagt ccttgacctg atttactttt tttaatttgc tagagacttt ttcattcagt
    30781 acactttcgt atacattttt aagttcttca ccgattttat tgatacttcg ctcttttgct
    30841 gtttgataac cggcttgtgt cgtactcggt agttcttgat tggtaatctg ttggatcagt
    30901 cggataaatt cttcgtttgt cttccccata tagcagttct tgctatcttc taaccagcca
    30961 ttatacactg gaatatctcg aacaagtacg ttttgtttgc ttgctaaagc ttctaaaaca
    31021 acgatccctt ctgtctcttc ataagaaggg aaaaagaata gatctgcacc tgaatacgca
    31081 ccttctatga tctctccacg aatgtaacca ggaaacagga cattttcagg atgatcttct
    31141 tttacaattt tccggatgtt ccgaggaatc gagtacatgg gaacatgacc aaaccaaata
    31201 aatgtgtatt cgggcatctt cttagcaatt tctacgaaat caatgattcc tttacgttcg
    31261 aaaaacaaac cgacacaaat gattactttt tgatccggag acagtttgaa atattctctg
    31321 aacttttgtt ctttcattgg atcagggtga tatttctcta agtcgatccc attggaaata
    31381 gcttgaatcg gcacacggat accatagctt tccaacaact gttttgaata aggtgttggc
    31441 gtgatcagat agtccgcttt ttggtacaat cctaccagat atttttttac gataggagaa
    31501 agctggttcg atccgataaa ggaatttcgg aaatcttcct ctgtagaatg cgcatgatag
    31561 acaacttttt ttccttctct tctggcttta ttgaccatat tatgactatt gattccgtat
    31621 gtattgatgt gcaggatgtc ataatcttct ttttcatcta gtgtataaga gataccgact
    31681 tccgtcagag cacgtttttg atgatcaagc gcacgcccga ttccagattt tgcaagcatc
    31741 ttttcacttt caaaatataa taataccttc accttacgtc ccccttcact tatatagtat
    31801 aacatagcct acaaactgtc actcaaactt aagcc
//
LOCUS       DACEEZ010000023        31621 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20313_2017
            SAMN12639966-rid9879223.denovo.023, whole genome shotgun sequence.
ACCESSION   DACEEZ010000023 DACEEZ010000000
VERSION     DACEEZ010000023.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639966
            Sequence Read Archive: SRR10023158
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 31621)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 31621)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741683.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741683.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:44:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,016
            CDSs (total)                      :: 2,962
            Genes (coding)                    :: 2,889
            CDSs (with protein)               :: 2,889
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 36 of 73
            Pseudo Genes (incomplete)         :: 29 of 73
            Pseudo Genes (internal stop)      :: 18 of 73
            Pseudo Genes (multiple problems)  :: 8 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31621
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639966-rid9879223.denovo.023"
                     /isolate="E20313_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(208..2100)
                     /locus_tag="IWO89_001143"
     CDS             complement(208..2100)
                     /locus_tag="IWO89_001143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ0455913.1"
                     /translation="MSKTNTRPSHGPAGGPGHGGPGRNLGVKGEKPKNFWGTVRRLFG
                     YMSKRMVAIIAVFVLAIAAVIFQIQTPKVLGKATTEIFKGVMAGAQQMQQGQQVTKFP
                     IDFDKIAQIIATVIVMYLISAVFNFLQQFIMTRVSQRTVYELRHDLEAKMNRVPISYY
                     DTHTNGDIMSRAINDMDNIASTLQQNLTQFVTSAVTLIGVIVMMLSISWQLTLVALLM
                     IPLSLIIVMIVAPKSQVFFADQQKSLGLLNNQVEETYGGHTIVKTFNHTEKDQEVFEK
                     ENQNLYAAGWKAQFISAIIMPLMNFVKNLGYVFVAVLGGVKVANGNMTLGDVQAFLQY
                     TTQFSQPITQLASLMNTIQSTVASAERVFEVLDEEEMSNEKSGLPEEKDTPYKVRFEN
                     VQFGYTPDNLLMTDFNLDVKPGEMVAIVGPTGAGKTTLINLLERFYDVSGGSIRYDGV
                     DTRDLSRDELRAQFSMVLQDTWLFTGSIYDNIKYGNDDATDEQIIEAAKAAHVDDFVR
                     KLPEGYNTVLNEDASNISQGQRQLITIARAFLANPDVLILDEATSSVDTRTEILIQKA
                     MNRLLENRTSFVVAHRLSTIRDADNIIVMNHGSIVETGNHDTLMEKDGFYADLYNSQF
                     SEEEAS"
     gene            complement(2097..3824)
                     /locus_tag="IWO89_001144"
     CDS             complement(2097..3824)
                     /locus_tag="IWO89_001144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ0455914.1"
                     /translation="MLKIVKRISLTSAIAAAVFMVIQVLADLYLPTLTSNIIDKGVAQ
                     GDVDYIWHTGFVMIGFSLISILAAIANTFFATRESQKLGKQLRTDVYKKSESLTKDAF
                     DKYGTASLITRTTNDVTQIQMVTQMFLRMMINAPITLIGASILAYQKDHQLTKIFLVV
                     IPVMIILIGGIMYFAVPLFKSMQKKTDRLNLVFREGLTGVRVIRAFDKTRFEENRFDL
                     ANKDYTNTAIKVNTIVALMMPMMTLIMSGTNVAITWFGGHYIADMTLEVGNLIAFMTY
                     AMQILISFMMLSAIFIMVPRAQASADRINEVLDEKIGIHDPENPKTVSFAGKNATLAF
                     NHVNYRYHGAEKLALEDIDFQAKSGEIVAIIGGTGSGKTTLVNLIPRLYDIESGSIQI
                     NGTDISDMTQYNLRELMGFVPQKAVLFSGTIRDNMQYGKPDATDEMIWKALEIAQAKD
                     FVSEMEDGLDSHVEQGGGNFSGGQRQRLAIARALVKTADIYVFDDSFSALDFKTDANL
                     RQALKTNMKESITVLVAQRVSTVMDADMILVLDEGKLVGKGTHEELLATNETYQEIVH
                     SQLREEDLA"
     gene            complement(3840..4490)
                     /locus_tag="IWO89_001145"
     CDS             complement(3840..4490)
                     /locus_tag="IWO89_001145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HAQ0455915.1"
                     /translation="MPTQTFFHLPKEKQKRLIEAARIEFSRVPLKEASIANIVKLADI
                     PRGSFYQYFEDKEDLYFYYFETMRRDSSKDMLQLMKESDGDLFKGFESYFSKMIYEIL
                     EGENASFYRNLFMNMDYRASRKVAPHTSRQKTPHDPHKEHKQDAKELLELIDRTKLKV
                     KNDHELELLIQLVMNTVFTTIAHSYRLFSTSEDYSVEQAISEFNLKLSWLKNGVYK"
     gene            4694..4990
                     /locus_tag="IWO89_001146"
     CDS             4694..4990
                     /locus_tag="IWO89_001146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HAQ0455916.1"
                     /translation="MAQSISVQDFNKLPLDADSIVLDTRNTLDYTKNHLSHSLSIPAA
                     ILPRQLKNLDPKKLYYILSYTGRRSEALADQLTANGFRAVYIIGGMEALQSSVA"
     gene            5162..5782
                     /locus_tag="IWO89_001147"
     CDS             5162..5782
                     /locus_tag="IWO89_001147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-dependent NADH-azoreductase"
                     /protein_id="HAQ0455917.1"
                     /translation="MASVLVVKGHPLTAEESRTVKALTSFLTSYKENHPDDEVTVLEL
                     YRDDIPEIDEELLSGWEALRAGAEFTSLSESQQQKIARFNELTDQFLAADKIVIANAL
                     WNLNIPTKLKAWFDTVNVAGKTFRYTENGPEGLVTGKKALHIQSNGGVYNGQDFASQY
                     VKGILNFVGIDQVDQLFIEGIDYDPDRADELMQNALDKAAALGKSF"
     gene            complement(5874..7040)
                     /locus_tag="IWO89_001148"
     CDS             complement(5874..7040)
                     /locus_tag="IWO89_001148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HAQ0455918.1"
                     /translation="MEGKNKKNRPVSWFWRWFLNNQVVTALLVVLLILLIVFLFTKVS
                     YLFAPIWQFLAIVGLPIILAGILYYLMNPVVDYFEKRKVPRLYSIIGLFVLVVALIAW
                     GSVVIIPKIQEQTVSFISNFPQYVDTIDNKLTEILRDPLFNQFREQLETAGDKFMNSA
                     GDMIQDISKSTVQSLGSFVGAVATILVALLTMPFILFYLLKDGKQLAPYFVKFLPTRM
                     QKPTLNVLSEMNSQVSSYIRGQLTVAFAVAIMFMIGFAVIGLEYAVTLGIIAGFLNLI
                     PYLGSFLAMIPAIFLAIVAGPFMIIKVLIVFAVEQTIEGRLISPLVLGNQLAIHPVTI
                     LLVLLTSGKLFGIIGVILGIPVYAAAKVVITHVFEWYTTISSLYEENPLKKQKE"
     gene            complement(7052..7405)
                     /locus_tag="IWO89_001149"
     CDS             complement(7052..7405)
                     /locus_tag="IWO89_001149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIA"
                     /protein_id="HAQ0455919.1"
                     /translation="MIQAKITEIGTEAINKEEPMLILFDKTATAALRKYSVIQEVSKD
                     EPFSLKAGGTIAFDDQVYTIDYVGPMANGNLETVAHVSLIFDACPEEGQIANGIYLHP
                     HRLPAIGLGSQIFYN"
     gene            7533..8555
                     /locus_tag="IWO89_001150"
     CDS             7533..8555
                     /locus_tag="IWO89_001150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactonase family protein"
                     /protein_id="HAQ0455920.1"
                     /translation="MIEKIILGTYTRRESKGIYTIDLDTEKEELANLSLAVEENSPTY
                     VAKSKAGNLYTVTSVDGLGGAGAYDKDFHFLNAVTESGAPLCYVAVDEARQLLYGANY
                     HKGEVNVYHILGDGELKAADSIFHQEATGPHKNQDHAHVHYTDLTPDQRLVVCDLGTD
                     RVYTYDVSENGKLNLVTTFTAEPGTGPRHLVFHPNGQFAYLFGELDSTVRVLSYNKET
                     GAFEELQKISTLPEEFDGENGGAAIRISNDGKFLYASNRGHNSIAVFAVFEDGSHIQN
                     KQIISTEGDFPRDFALDPSNNFVVCANQNTDNLTLYRRHPETGLLELLKKDVYVPECV
                     CVYFDR"
     gene            complement(8994..9428)
                     /locus_tag="IWO89_001151"
     CDS             complement(8994..9428)
                     /locus_tag="IWO89_001151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0455921.1"
                     /translation="MLPNIGYYVQSIDQIVKETEAIGEGMHPKYEVIRNAIDEGKTSE
                     LDKSTLEEIILLFEEGTKKYRAMLKKVSTLRPPAKAMGIHKNFEKAYLSYVAGCEEMI
                     QSIDLESGVDEDLFNTSEAKQDQATDQLTAAIQKMTNLLLKK"
     gene            complement(9538..11397)
                     /locus_tag="IWO89_001152"
     CDS             complement(9538..11397)
                     /locus_tag="IWO89_001152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HAQ0455922.1"
                     /translation="MKRYISRLSTKQKKILKGTAAIISIFFLVVFSNLFLQWCQNDLS
                     VDLALKFAFSWHTEKFFLACLVLLIILIFLIALAGSVPLGSLTYVVAIGVLGFANYMK
                     MSYRQEPIYPDDLKMITEIGLLKDMTGTMLFTVILAAAGTVIGLFCWYMFRSLKKGRR
                     FQLIRLTTLLVAIGLLGYISNFNNPDNLLRKAYNKTALWIPYSQKMNYYNTGFIGGFL
                     YNLKVEPMDEPEGYSKEKIKEITEKYQKLADEKNKTVEEESPNIVFVMSESFSDPSRL
                     NGVEVSGEPLADYYEVADQTYSGNMLSQNYGGGTANIEFEALTGFSMALFNAQLTTPY
                     TMLVPKMDQLPSIVSALNAQSYQTTAIHPYNTSMYKREDVYQTLGFDQFISERTMTYT
                     DTIENNPYISDESAYKEVMTLLKEEKTPQFIHLVTMQTHMPYDGKYDNLDYPAKTSDG
                     SGTLDLENYLQDISYSSTALKQFTEELKNLSRRTLVVFWGDHLPGIYSDTIQAKNDKQ
                     TLHETQFLMFDSKGELEKQTTHDAITSPFYFAANLMEQTNQTTNGFYQLLLSLEQELP
                     AFERELYYQNGQWYKEAQFNRSQQEIYDEYQLIQYDIVAGKQYSLADGFFEHE"
     gene            complement(11515..12675)
                     /locus_tag="IWO89_001153"
     CDS             complement(11515..12675)
                     /locus_tag="IWO89_001153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-3-phosphoglycerate dehydrogenase"
                     /protein_id="HAQ0455923.1"
                     /translation="MYHVHLIDAIPEQEIEKLQGEYFKIIDDLPAEGIVIRSTKVKDE
                     WLTPDLLAISRAGVGVNTINVEKASENGTIVMNTPGVNANAVKELVLCCLLLSSRPII
                     EASRMVQTLTGPNILEQAENKRSAYVGRELQGKTIGLLGLGAIGTKVALSCYSLGMDV
                     LGYSIRDAQLDYVRQADLETVLSTSDYIVVMLPLTEDTKGLIDQANIEKMKKDAVLIN
                     VGRSEIVDKYAVMQALEKQHLAKYLTDFPEEEFLENDRILMLPHLGGSTQEALADSGR
                     LAVEALKDYLLFGTVREAVNYPSARMFFQAPFRFTIFYQKKTNILAEIFSLLNENELA
                     IADISRNHKNEHVYILIDIDSTDFEQLNQVKQQLEKISGVRKVRLLKKPERP"
     gene            complement(12982..14406)
                     /gene="araA"
                     /locus_tag="IWO89_001154"
     CDS             complement(12982..14406)
                     /gene="araA"
                     /locus_tag="IWO89_001154"
                     /EC_number="5.3.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-arabinose isomerase"
                     /protein_id="HAQ0455924.1"
                     /translation="MLNIGEKEFWFVVGSQHLYGEEALREVKKHAQEMVDELNENGQL
                     PYPIRLQELAVTADTITKIMKEVNYREEVAGVITWMHTFSPAKMWIRGTKLLQKPLLH
                     LATQYNESIPWKTIDMDFMNLNQSAHGDREYGFINARLNKQNKIVVGYWKRPEIQKEI
                     ADWMDVAVAYNESFGIKVARFGDNMRNVGVTEGDKVEAQIQFGWTVDYFGIGDLVQVI
                     DRVSDEEVDQLFEEYKELYTFDYGDYEEKTWEEHVKVQAQQEIGIRRFLEEGGYNAFT
                     TNFEDLYGMKQLPGLAVQRLMAEGYGFAGEGDWKTAAIDRLLKIMARGKDTGFMEDYT
                     YELASGQEAILESHMMEVDPTLAATKPRIVVSPLSMGDREDPARLVFDGKAGEGVVVS
                     MADFGTHYKLLINEVEAFEPTTEAPNLPVARVLWKTKPNFHEGVHSWIQAGGGHHTVV
                     SLNLTTDQIETWAKLVELETVVIR"
     gene            complement(14422..15120)
                     /locus_tag="IWO89_001155"
     CDS             complement(14422..15120)
                     /locus_tag="IWO89_001155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005873905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase"
                     /protein_id="HAQ0455925.1"
                     /translation="MLEQLKEEVYQANLDLPKHGLVKYTWGNVSAFDPETRYFVIKPS
                     GVSYEEMTADDMVVVDLDNHIIEGKLNPSSDTPTHAVLYRSFPQIGGIVHTHSTWATI
                     WAQAGLDVPAMGTTHADTFYGSVPCARFLTQQEIDSGYEYETGKVIIETFKERKIDPL
                     AIPGVLLHGHGPFTWGKDAQSAVMNAVVLDEVCKMNLFTRQLNSFSEELPQRILDKHY
                     LRKHGKNAYYGQNK"
     gene            complement(15077..16738)
                     /locus_tag="IWO89_001156"
     CDS             complement(15077..16738)
                     /locus_tag="IWO89_001156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FGGY-family carbohydrate kinase"
                     /protein_id="HAQ0455926.1"
                     /translation="MKEVNAQSMLDVIDGRTSLGIEFGSTRIKAVLIDSSFTPIAAGS
                     YEWENKLENGIWTYSLEDIWKGLRVSYRKMAAEVFEIYGENLTTIGSIGFSAMMHGYL
                     AFDSKEQLLVPFRTWRNTITEEAERILTEKFQYNIPQRWSIAHLYQSILNKEEHVKEI
                     NFLTTLAGYVHWQLTGEKVLGVGDASGMFPIDPSTKTYHQEMKNIFGQLIEKAHVAID
                     LDHILPKVLVAGEEAGKLSESGAYLIDPTGNLRPGIPVCPPEGDAGTGMVATNSVAKR
                     TGNISAGTSAFAMIVLEKELENVHPEIDMVTTPSGELVGMVHTNNCSSDINAWVSIFE
                     EFTNSLGMEISRDKLFTVLFNKALEGDTDCGGLLSYGYFSGENITGVNEGRPLFVRTP
                     GSRFDLANFMRIHLASAFGAMRIGMDILKSENVQIDRLVGHGGIFKTPEVGQRILASA
                     MEAPVTVMDTAGEGGAWGIALLAAYMMDRHGKSLENFLAEDVFGQDQGITITPSAEEI
                     AGYQIFMRRYREGIDIEKTAIHQLQEKGVIENAGTTERRSIPSKS"
     gene            16956..18356
                     /locus_tag="IWO89_001157"
     CDS             16956..18356
                     /locus_tag="IWO89_001157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar porter family MFS transporter"
                     /protein_id="HAQ0455927.1"
                     /translation="MKKEKKVSSSFIYFFGAFGGILFGYDIGVMTGALPFLQHDWGLA
                     GKASLIGWITSSVMLGAILGGSLSGLLSDKLGRRKMILLSALIFMAGSVLSASAPHNG
                     SYFLIAARILLGLAVGAASALVPAYMSEMAPARLRGRLSGINQVMIASGMLLSYVADY
                     LLKVLPETMAWRVMLGLAAVPALILFFGVLALPESPRFLMQSGRLEEAKRVLNYIRTP
                     KEAEQEFEQIQLNVKQEKTTGTSWHTLFLEKYRSLVIAGIGVAVFQQFQGANAIFYYI
                     PLIVEKATGHAASDALMWPIIQGIILVAGSLLFLVIADKFNRRTLLKIGGSVMGLSFI
                     LPAVLGTVLDAHTNSLLILLFLCIYVAFYSCTWAPLTWVIIGEIFPLAVRGRASGLAS
                     SFNWIGSFLVGLLFPVMTASMSQEIVFGIFGIICFLGVLFIQEIVPETRGKSLEEIEQ
                     SASKKTYPKRISIHHS"
     gene            complement(18608..19687)
                     /locus_tag="IWO89_001158"
     CDS             complement(18608..19687)
                     /locus_tag="IWO89_001158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAQ0455928.1"
                     /translation="MAKYQDIAEEIKQKILREEYEVNTTIPSEFKLQEIYDVSRHTIR
                     QAIAVLVNEGYLRKEKGSGTYVDDSYKKEKTPLNRTKTIGVITTYLSDYIFPSIIRGI
                     ENELRESGYSLLLASTNNDHELEKECLKKMIAYGVDGLIVEPTKSNQYNPNLALYVAL
                     REQGIPMVMINAAYEELNASYICLDDVKVGFMAAEYLIKKGHQRLLFITKIDDLQGKY
                     RMKGFISACEQYNVRFLTEDIITFTTETRETIAEKTVQRLKEEQALDGIICYNDQIAS
                     LMLDKLTENGYHIPDDLSIVGTDDSSLSHLGNKKLTTLTHPKEKLGQDAARWIVTTIE
                     KGQAQPNILYEPVLVERETVKENEK"
     gene            complement(19773..20732)
                     /locus_tag="IWO89_001159"
     CDS             complement(19773..20732)
                     /locus_tag="IWO89_001159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 43 glycosylhydrolase"
                     /protein_id="HAQ0455929.1"
                     /translation="MMKTLYNNPIVRQRADPWVYKHTDGYYYFTGSVPGYQVIELRRA
                     KSLNELEHAASLIVWQAHEEGPMSELIWAPEVHYINGKWYIYFAASNHKTIRDESHHH
                     RMFVLENQHIDPMNTDWIEKGQIKTKRETFSLDGTTFSSQGKLYYVWAQQDPEITGNS
                     NLYLSEMDNPWTLKGKQILLSIPEYEWEKRGFSVNEGPAVLTRNGKIFITYSGSATDE
                     NYAMGLLWADEHKNLLLKESWHKLSEPVFVSSEKNKQFGPGHNSFTVSEDGCFDILIY
                     HARPEKNQKGDPLDNPNRHANAQCFTWNEQGFPEFGEPVPYAL"
     gene            complement(20744..22267)
                     /locus_tag="IWO89_001160"
     CDS             complement(20744..22267)
                     /locus_tag="IWO89_001160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-N-arabinofuranosidase"
                     /protein_id="HAQ0455930.1"
                     /translation="MDEEVKVKIREDFTVGEISSRLFGSFVEHMGSVVYTGIYEPGHE
                     TADGDGFRQDVLSYVKELELGVIRYPGGNFTSGYDWVDTVGRKEQRPKKIDLAWRGIE
                     PNSFGLHEFFKWISHVQAKPIFTVNLGTKGIDEAKNLVEYCNFPKGTFWSDLRIKNGQ
                     KKPFGVKLWCLGNELDGPWQVGAKSAYEYGRLANEASKVMKLVDDSIETILVGSSTPR
                     LPSYPEWDRIALEEAYENVDYLALHNYIDKYDEEDLTKPPKNERDDTATYLAKAYRFD
                     RQIEEIVATCDYVKGYLRSEKTMYLAFDEWNVHAQPEKRHRDFEVGSPIDWCYFTMED
                     TLLFGSLGLAILRHADRVKISCQSLLVNTIPLILTDAKGQAWRNPTYYVMQHLSKYAK
                     GQVLKQQIICPVYDCEKIKNVPYVDSVIVDNGNQLTIFLINRTNEQQKITIEHDFNLL
                     DQGEHYTLTSSDLLDKNTREEPEKIKPEINYFSIEKHDELNVGIEKYSWNVIRIKKM"
     gene            complement(22277..22840)
                     /locus_tag="IWO89_001161"
     CDS             complement(22277..22840)
                     /locus_tag="IWO89_001161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YesL family protein"
                     /protein_id="HAQ0455931.1"
                     /translation="MKIVETKFYGQLIVVSDYILLGILWVIVCLPVITIVPATCAVFY
                     VMAQWKGEGSGNIIYFFFQGIKQHFGKNLFLSLLTIIIYFLVNQLLEEQTIVLSISGY
                     VVLLIYSMFLLSWIYVISENKDITFLQIFEQSAWKVFFDFLRNILCCVLLLGFVLLIF
                     LFPPFIFIFAGGVWKLVSIILTIGNER"
     gene            complement(22846..23709)
                     /locus_tag="IWO89_001162"
     CDS             complement(22846..23709)
                     /locus_tag="IWO89_001162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HAQ0455932.1"
                     /translation="MAPESSITVTVNSRKAVSGKKIIRTISMLVILLVIAFPFLWLII
                     SSFKHEKDIISFPPRIFADSYTLDNYIKVWTTIPLLDYIKNTVIFAGGTVITSVFFDS
                     LAGYAFARMRFKGKSVLFYFVLLTMMIPFQVFMIPLFIEVNLLGMLDTYAGLIIPRMT
                     TAFGIFMMRSFFITLPDSLEEAARIDGLSEFNIFLKIMLPLSKPTLLSLGIFTLMNSW
                     NDLLYPLILTSSSKMRTLPAGLALFTGQNISFYGPVMAGTVISMLPLLVVYIFAQKYF
                     VQGTAMSGMKE"
     gene            complement(23723..24619)
                     /locus_tag="IWO89_001163"
     CDS             complement(23723..24619)
                     /locus_tag="IWO89_001163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAQ0455933.1"
                     /translation="MNGFTKYWNRPDRAAYLFLTPSFLVLLLFTVIPLLGTFAISFTD
                     LNIFFTSRNFVSFENFTRIFSDERAVNSLIHSLYFTVLETPTQIIVGLLAAVALSKNT
                     RFNRFCRSTFYIPVICSLTSIAIIFSMLLDPNVGGIPYLFSQVGLPIPQFFRDPTLAM
                     PTVAIMTVWKNFGVTMTILLTAIQGISPSLYESAQMDGATNRQQFFNITLPQIVPSLG
                     FCILTNLIGSMQVFDQVYVATAGGPQFKTETAVQYIYSRGFTAPYELGYASALSSVLF
                     IIVATIAISTNVYMSRKEKQMK"
     gene            complement(24794..26065)
                     /locus_tag="IWO89_001164"
     CDS             complement(24794..26065)
                     /locus_tag="IWO89_001164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HAQ0455934.1"
                     /translation="MKGKKWLIGSLLVAATGILGACGGGSSAESDSDKEVTLTFWNGF
                     TASDGEILQEIVNEFNKTNDKNIKIEMDIMTWANLNEKLPTAISSKKAPDFIALNYPD
                     FAQYVENGAVQPLDDFWEYDGVDKSDFKETAIELGEVEGKQYFVPMQVQGMYMYWNND
                     LFKQAGLDTEQPPTTWEELTEMAPKLTDSSKNVSGFVFNKDGTAPLYNWMLANGGKLV
                     NDDYTKSEFASPENLKTLKAIQKMIHVDKTGPESISGAEMDNLMNAGQLAIEINGPWL
                     NNGLKANEIDYNVTTLPQGSDSDKKTAILDGVGYAIPASTDNNKKEAVYEFLKYWNTT
                     EIGKKWSIENGFPAYLDSVAKDEEVKNDPIVSELSKQMDYAEPYLPGFTQISAINNDI
                     INPLIEKLLAGEDPETLMNDADDAINQLLQK"
     gene            complement(26055..27572)
                     /locus_tag="IWO89_001165"
     CDS             complement(26055..27572)
                     /locus_tag="IWO89_001165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-N-arabinofuranosidase"
                     /protein_id="HAQ0455935.1"
                     /translation="MEATMLISSVKRVGKVDPRLYSSFIEHMGRAVYEGIYQPDHPSA
                     NKSGFRQDVIHLVKELNIPMIRYPGGNFVSGFRWEDSIGPIANRPRRLDLAWRTIETN
                     EVGIHEFYNWCQHVGAEINMAVNLGTRGVDAARNLVEYCNFPKGTYWSDLRRSNGQES
                     PFGIKTWCLGNEMDGPWQIGHKTAEEYGRLAEETAKAMKLVDDSIEVVLCGSSNRQMP
                     TFGDWELTVLDQAYDQIDYLSLHQYYGNQKNDLSHYLARSLDMDRFISEVTAMCDTVK
                     AKKHSKKQINLSFDEWNIWYHSAEQDEQAAPWQIAPPLLEDNYNFEDALLLGCLLITL
                     LKHADRVKIACLAQLVNVIAPIRTETNGEAWRQTIYYPFMQAAVYGQGEVLSPQIRSA
                     NYATEDFEEVPYLESIAVINDKELVIFAVNRGEEEMNLTVHLSELSLEGVIDFSEMSG
                     FDTKATNLPKSEQVKPSHSTNVKAEDGKINCLLKPLSWNVVRCRLSDEGGTKNER"
     gene            27770..28660
                     /locus_tag="IWO89_001166"
     CDS             27770..28660
                     /locus_tag="IWO89_001166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArsR family transcriptional regulator"
                     /protein_id="HAQ0455936.1"
                     /translation="MQLQINEESLPVYEALASKTRIKIIQLLSKKKMNVKDLAKELGV
                     SSAITTMHVKKLEEANIIKTEKVGQQKISSLRVDKIDISFPEKIFNAFDTKETSIPIG
                     HYTNYAIEPTCGLATIHDFIGKVDEPRYFMDPRRMDARILWFTSGFVEYQAPNFLNTE
                     DTLEMLEVSVEISSEFPFSNDNWPSDITFSLNGVELGTWTSPGDFADIRGKYTPDWYP
                     DNLNQYGLLKTIRITKHLTNMNGEPLSNITINDIPKEQDTWHLRIEVKDDAKHVGGCT
                     LFGKGFGNYDQDIKLKVYYS"
     gene            complement(28733..29557)
                     /gene="nadE"
                     /locus_tag="IWO89_001167"
     CDS             complement(28733..29557)
                     /gene="nadE"
                     /locus_tag="IWO89_001167"
                     /EC_number="6.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ammonia-dependent NAD(+) synthetase"
                     /protein_id="HAQ0455937.1"
                     /translation="MSLQTEIINELGVKPTIDPKEEIRKSIDFMKAYLKKYPFLKTFV
                     LGISGGQDSTLAGRLAQLTMEEMREETKDENYQFIAVRLPYGEQADEEDAKAALDFIQ
                     PDVSLRVNIKPAVDAQVQVLSEAGVGISDFNKGNIKARQRMITQYAVAGERAGAVLGT
                     DHAAENITGFFTKFGDGGADILPLFRLDKRQGKQLLQALNAPEKLYTKIPTADLEDGK
                     PMIADEVALGVTYNEIDDYLEGKEVPAQAQEKIEAWWNKTQHKRHLPISVLDDFWK"
     gene            complement(29569..31041)
                     /locus_tag="IWO89_001168"
     CDS             complement(29569..31041)
                     /locus_tag="IWO89_001168"
                     /EC_number="6.3.4.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate phosphoribosyltransferase"
                     /protein_id="HAQ0455938.1"
                     /translation="MNNTYPDDSLTLHTDLYQINMMQTYWELGRADLHAVFECYFREM
                     PFNHGYAVFAGLERLVNYLENLTFNDSDIAYLRGLEVYPEGFLEYLQNFEFKATVRSA
                     REGELVFANEPLIQVEGPLAHCQLVETALLNMVNFQTLIATKAARIKSVIGEDPLLEF
                     GTRRAQELDAAVWGTRAAYIGGADATSNVRAGKIFGIPASGTHAHSLVQSYGNDYEAF
                     MAYAKTHKDCVFLVDTYDTLKSGVPSAIRVAKELGDKINFQGVRIDSGDMAYISKRVR
                     EQLDAAGFTEAKIYASNDLDEATILNLKMQKAKIDVWGVGTKLITAYDQPALGAVFKL
                     VSIEDDEGKMMDTIKLSSNAEKVTTPGKKQVWRITRNFDGKSEGDYVTLWDEDPREEE
                     AIFMFHPVHTFINKTVRDFTARPVLQDIFIEGKRVYELPALNEIKEYTKENLDSLWEE
                     YKRDLNPQKYPVDLSTECWNHKMAIMERMKKSVAELHTEA"
BASE COUNT         9255 a         6637 c         5467 g        10262 t
ORIGIN      
        1 ttttgaacac cgtttaagga agaggttgta aaatcagcgg tttgacctga gtattagaga
       61 tacaaaacgg aaaaatagct tctcatattt tgccgttttt tgagctaata ccgaaggaca
      121 gctgattgaa caccgtttat tcggaaaaag ggactgtgac acgacttttg tcacagcccc
      181 taaaaaaaat aataaatttg tactattcta tgaagcttct tcttcagaaa actgactgtt
      241 ataaagatct gcatagaatc cgtctttttc catcaatgta tcgtggtttc ctgtttctac
      301 gatcgaacca tgattcatca caataatgtt gtctgcatcg cgtattgtag acaagcggtg
      361 agcaacaacg aaacttgtcc ggttttccaa taggcggttc atcgcttttt gaatcagtat
      421 ttctgttcgt gtatctacac tagatgttgc ttcatcaagg atcaggacat ctggatttgc
      481 taagaatgcc cgcgcaattg tgattagctg acgctgccct tgcgaaatat tgcttgcatc
      541 ctcgtttaat acagtgttat atccttctgg caatttacga acaaaatcgt ctacgtgagc
      601 agctttagca gcctcaatga tttgttcgtc tgttgcatcg tcgttcccat atttgatatt
      661 gtcatagata cttcctgtaa acagccaagt atcttgcagt accattgaaa actgtgcgcg
      721 caattcatca cgtgaaagat cacgggtatc tacaccatcg taacgaatac tacctccaga
      781 aacatcgtag aaccgttcaa gaaggttgat cagtgtcgtc ttccctgcac cagtcggacc
      841 tacaatagcg accatctcac caggtttgac atccaagtta aaatcagtca ttagcaagtt
      901 atctggtgtg tagccgaatt gaacattttc aaaacgaact ttgtatggtg tatctttttc
      961 ttctggaagg ccagattttt cattggacat ctcttcttca tccaacactt caaatacccg
     1021 ttcggctgaa gcaactgttg attggatagt gttcatcaaa cttgcaagtt gagtgattgg
     1081 ttgagagaac tgtgttgtat attgcaagaa cgcttgaaca tcccctaatg tcatgttccc
     1141 atttgcaact tttactccgc ctagtacagc gacgaacaca tagcctagat ttttaacgaa
     1201 attcatcaac ggcatgatga ttgccgaaat aaattgagct ttccaaccag cggcatacag
     1261 attctgattt tctttttcaa atacttcctg gtctttttca gtatgattga atgttttaac
     1321 aatggtatga ccaccatatg tttcttctac ttggttattc aataaaccta aacttttttg
     1381 ctgatctgcg aaaaacactt gtgatttcgg cgctacgatc atcacgatga tcaagcttaa
     1441 agggatcatc aataacgcga ctaacgtcaa ttgccagctg attgaaagca tcatcacgat
     1501 gaccccaatc aatgtgactg cactagttac aaattgagtc aaattttgct gcagcgtgct
     1561 tgcgatgtta tccatatcat tgattgctcg agacatgata tccccatttg tatgtgtatc
     1621 ataataggaa atcggtacac gattcatttt ggcttccaaa tcatgacgta attcataaac
     1681 tgttcgttgg gaaacacgtg tcataataaa ttgctgtaaa aagttgaata cagcagaaat
     1741 caaatacatc acgattactg tcgcaatgat ctgcgcgatt ttatcaaagt ctatcggaaa
     1801 ttttgttact tgttggcctt gttgcatctg ttgagcgcca gccattacac ctttgaatat
     1861 ttctgtggtt gctttcccca agacctttgg tgtttggatt tggaagatca cggctgctat
     1921 tgctagtaca aatacagcga tgatcgcaac catacgttta gacatatagc caaacaatcg
     1981 gcgtaccgtt ccccagaaat ttttaggctt ttcacctttt acaccgagat ttctgccagg
     2041 tccaccatgt cctggaccgc ctgctggtcc atgagaggga cgtgtgtttg ttttactcat
     2101 gctaagtcct cctctcttag ttgtgaatga acgatttctt gataagtttc gtttgttgct
     2161 aatagttctt catgtgttcc tttgccaacc agttttcctt catctagcac aaggatcata
     2221 tctgcatcca ttactgtact tactcgttga gcaactaaaa cggtgatact ttctttcata
     2281 ttcgttttca atgcttggcg aagattggca tccgttttga aatccaacgc tgagaaggaa
     2341 tcatcaaaca catagatatc tgctgttttg actaatgccc gtgcaattgc cagacgctgt
     2401 ctttgtccac cagagaagtt tcctcctcct tgttccacat ggctatctaa gccatcttcc
     2461 atctctgaaa caaaatcttt agcttgtgcg atttctaatg ctttccaaat catttcatct
     2521 gttgcatccg gcttaccata ctgcatattg tctcggatcg ttccagagaa cagaactgct
     2581 ttttgaggaa caaagcccat taattctcgc aaattatatt gagtcatgtc actaatgtcc
     2641 gttccattta tttggatcga accagattcg atgtcatata atcgagggat caaattgact
     2701 aaggtcgttt tacctgaacc agtcccccct atgattgcga cgatctcgcc cgacttggct
     2761 tggaaatcaa tatcttccaa tgcaagtttt tctgctccat gatatcgata attgacatga
     2821 ttgaaggcca atgttgcgtt ttttccagca aaagaaactg ttttaggatt ttcagggtca
     2881 tggataccga ttttttcatc caacacttca ttaatacggt cagctgaagc ttgtgcacga
     2941 ggcaccatga taaaaatagc ggaaagcatc ataaagctga tcaagatctg catcgcataa
     3001 gtcataaatg caatcagatt accgacttcc aaagtcatat ctgctatgta gtgtccacca
     3061 aaccaagtga ttgctacgtt tgtaccgctc atgatcaatg tcatcattgg catcatcaat
     3121 gcgacgatcg tattaacttt gattgccgta tttgtataat ctttattcgc taaatcaaaa
     3181 cgattttctt caaagcgtgt cttatcgaat gctcgaatca ctctgacccc tgtcaatcct
     3241 tctcgaaata ccaggtttaa acggtcagtt ttcttttgca tacttttgaa taatggaaca
     3301 gcaaagtaca tgattccgcc gatcaaaata atcattactg ggatgacaac taagaagatt
     3361 tttgtcaact gatgatcttt ttgataagca agtatgctcg cgccaatcaa cgtgattggt
     3421 gcattgatca tcatacgtaa aaacatttgt gtaaccattt ggatttgagt cacatcattg
     3481 gtcgttcttg tgatcaaaga ggccgttcca tatttatcaa acgcatcttt tgtcagactc
     3541 tctgattttt tatagacatc cgtacgcaat tgcttaccca gtttctgtga ttcacgagta
     3601 gcaaaaaatg tatttgcgat agcagctaag atactgatca aagaaaagcc gatcatcaca
     3661 aaacctgtat gccaaatata atcgacatct ccttgcgcta cccctttatc aataatattg
     3721 gaggtaagtg tcggcaaata taaatctgcc agtacttgga tcaccatgaa aactgcagct
     3781 gcaattgctg aagtcaagga gatccgtttt acgattttta gcatctagtt cccttctttc
     3841 tatttatata ctccgttttt cagccaagaa agtttcaaat tgaattcact gattgcttgc
     3901 tctactgagt aatcttcaga tgttgaaaat aagcgatacg aatgcgcaat tgttgtaaat
     3961 acagtattca tcactaattg gatcagtaat tccaattcat gatcattttt tactttcagc
     4021 ttcgtccgat cgatcaattc aagcaactct tttgcatctt gtttgtgttc tttatgtgga
     4081 tcgtgaggcg ttttttggcg actcgtatga ggagcgactt ttcttgaggc acggtaatcc
     4141 atgttcataa acaagttacg ataaaaagaa gcattctcgc cttccaaaat ctcataaatc
     4201 attttagaaa aataggattc gaatcctttg aatagatcac catcggattc tttcattagt
     4261 tgtaacatat ctttagagct gtcacgtctc attgtctcaa agtaataaaa ataaagatcc
     4321 tctttatctt caaaatactg atagaaactg cctctaggga tatccgctaa tttgacgata
     4381 ttagcaatcg aagcttcttt taagggtact ctagaaaact cgattctcgc agcctcgatt
     4441 agtctttttt gtttttcttt aggcaaatgg aaaaatgtct gtgtcggcat atggatcctc
     4501 ctttctttca agatgacaca ttgtcatatg acgctgtgtc attataacac ctcctacctg
     4561 ttttgcaaga catttgcata gtttttttca ttatcatccg atacattaaa aaagtgataa
     4621 gaggaatcct tccatttgaa ttaatgagag ttgcccatta aaatagccac atactaaaca
     4681 aaggatgaga taaatggccc aatcaattag tgtccaagac ttcaataaat tgcctctcga
     4741 tgctgacagc atcgttttag atacaagaaa taccttagat tacacaaaaa accacttaag
     4801 ccactcgctt tctatccccg ctgctatctt gccaagacaa ttaaaaaatc tagatccaaa
     4861 gaaactttat tatattctaa gctatacagg caggcgatca gaagcattag ccgatcaact
     4921 tactgctaat ggttttcgtg ctgtttatat cattggtggg atggaagctc ttcagtcgag
     4981 tgttgcttga atattgcttg ttagattcaa aagaggctgt gacaaaaaat cgtgtcacag
     5041 cctcttttat accctgtctt tttttagtta tttattcttc aaccttatca atatgacttg
     5101 atagcctttt ctttttctca tagttataac aatcatagca ataaaaggag aaggtaagca
     5161 tatggcatct gttttagtag tcaaaggtca tcccctaact gcagaagaat cgcgcacagt
     5221 taaggcattg acgtcttttt taacaagtta taaagaaaat catccagatg atgaagtgac
     5281 tgttttggaa ctttatcgcg atgatattcc ggaaatcgat gaagaattat tatcaggttg
     5341 ggaagcatta cgtgctggtg cagaattcac ttccttatca gaaagtcaac aacaaaaaat
     5401 tgctcgtttc aatgaattga ctgatcagtt ccttgctgct gataaaatcg ttatcgcaaa
     5461 cgccttatgg aatctaaata tcccaacaaa attaaaagct tggttcgata ccgtcaatgt
     5521 agctggaaaa acgttccgct atacagaaaa cgggccagaa ggattagtga caggtaaaaa
     5581 agcactgcac atccaatcta atggcggtgt atacaacgga caagactttg cctctcaata
     5641 tgtaaaaggt attttaaact ttgtaggcat cgatcaagta gatcagctat tcatcgaagg
     5701 aatcgattat gaccctgacc gtgcagatga gttgatgcaa aatgcattag ataaagctgc
     5761 tgctttaggt aaatcatttt aaaaattgag ataaatgcat acaaaagaga cagaggaaaa
     5821 aatcttcaaa gcagcatttg taccttggat attttcccat ctgtctcttt ttgttattct
     5881 ttctgctttt tcaacggatt ttcttcataa agagaagaga ttgttgtgta ccattcaaaa
     5941 acatgagtaa tcactacttt agctgctgca taaacaggaa tccccaagat cacaccgatg
     6001 attccgaaca atttcccaga agtcagcaga actaataaaa tcgttactgg atggattgct
     6061 aactgattcc ctagtactaa tggagagatc aagcgtcctt ctattgtctg ctctactgca
     6121 aaaacgatca gtactttgat gatcatgaat ggacctgcta caatggctaa aaagatagcc
     6181 ggaatcatcg ctaaaaatga tcctaagtaa gggatcaggt tcaaaaaacc cgcaataatc
     6241 cccaacgtga cagcatactc taaaccaatg accgcaaaac caatcataaa catgattgcc
     6301 acagcaaaag ccacagttag ttgtcctctt atataagaag aaacttgact attcatttca
     6361 ctcagcacat tcaacgtcgg tttttgcatg cgtgtcggta aaaatttgac aaaataaggt
     6421 gctaattgtt taccgtcttt caatagataa aataagataa acggcatcgt cagcagagcc
     6481 actaaaatag tcgctaccgc gccgacaaaa cttccaagac tctgaactgt actcttagat
     6541 atatcttgga tcatatcgcc tgccgaattc atgaacttat cacctgctgt ttccaactgt
     6601 tcgcgaaatt ggttgaacag cgggtcccgc aaaatctcgg ttaatttatt atcaatcgta
     6661 tctacatatt gagggaagtt actgatgaag ctcactgttt gttcctgaat cttagggatg
     6721 atcactacgc tcccccatgc gattagtgca acaaccaaaa caaacagccc aatgatactg
     6781 taaagccttg gtacttttcg cttttcaaag taatcaacta ccggattcat caagtaatac
     6841 aagattcctg ctaaaatgat aggcagacct acgattgcta aaaattgcca gattggtgca
     6901 aacaaatatg aaacttttgt aaataaaaag acgatcagca atattaacaa tacaacaagt
     6961 aaggctgtga ctacttgatt atttaaaaac cagcgccaaa accacgatac cggtctgttc
     7021 ttcttatttt ttccttccat aattcctcct gttagttata aaaaatttgg cttcccaaac
     7081 caatagcagg cagtctatgc ggatgaagat agattccatt tgcaatttgt ccttcttctg
     7141 gacaagcatc aaaaatcaat gatacgtgag ctactgtctc taaattccca ttggccatcg
     7201 gaccgacata atcgatcgta tatacctgat catcaaaagc aattgttccg cctgctttca
     7261 aagaaaaagg ttcgtctttt gatacttctt gaatcaccga atattttcgt aaggcagctg
     7321 ttgctgtttt atcgaacaaa atcagcatgg gttcttcctt gtttattgct tctgtcccaa
     7381 tttctgtaat ctttgcctga atcattgcac tctctccttt aattgctttc aaaaacagta
     7441 taacacattc tcattcttta gccgtgtgga ataattatgt gatagaataa aaacgaaaac
     7501 tatttcatta caattaggag gaatcagctc atatgatcga aaagataatt ttaggaacct
     7561 atacgcgtcg cgaaagtaaa ggaatctata cgatcgacct tgatacagaa aaagaagaat
     7621 tagccaacct ttcattagct gttgaagaaa atagccctac ctatgtagca aaaagtaaag
     7681 ctggcaatct ttacacagtt actagtgtgg atggattagg tggtgccggt gcgtacgata
     7741 aggattttca ttttttgaat gcggtaacag agtctggcgc tcctttgtgt tatgtagcgg
     7801 ttgatgaagc acgtcaatta ctatacgggg cgaactatca taaaggtgaa gtaaatgtct
     7861 accatatatt aggagatggc gaactgaaag cagcagattc aatattccat caagaagcga
     7921 caggtcctca taaaaaccaa gatcatgcgc atgtccacta tacggatctg actcctgacc
     7981 aacgattagt cgtatgtgat cttggtacag atcgtgtgta tacttatgat gtgtccgaaa
     8041 acggcaaatt gaatcttgta actaccttca ctgcagagcc aggaacaggc cctcgccatc
     8101 ttgtctttca tccaaatgga caattcgctt atttatttgg tgaattagac agcacagtcc
     8161 gcgtgctttc ttataataaa gaaacaggtg cttttgaaga attacagaaa atatcgacct
     8221 tgccagaaga attcgacggt gaaaatggag gggcagctat ccgcatttca aatgatggca
     8281 agttccttta tgcttctaac cgaggacata actcgattgc tgtattcgca gtttttgaag
     8341 atggttccca cattcaaaac aagcaaatca tctcaacaga aggggatttt ccccgcgatt
     8401 tcgcactaga tccaagcaat aattttgtcg tgtgtgccaa tcagaacacc gataatctga
     8461 cattatatcg ccgtcatcct gaaacaggat tactggaatt actgaaaaaa gatgtctatg
     8521 taccagaatg tgtctgtgtt tattttgatc gttagtaaga aaaggagaat gaaatgaatc
     8581 agcaagacgt attagacaga ctacgggaag aactaaatat tcctttcttt gatgggaaaa
     8641 tcgaagataa agagtatagc gaagaggaat atcagaaact caaagccgat ttacagaact
     8701 actttgaaca atatgtacag aatgtagaaa actaatcagc tttccatact atctgaaaag
     8761 aacatcagga caagactttt ccgtctatcc tgatgttctt ttctacaaat aaaaaaccgc
     8821 cgagatggtt agactcgacg gaaaaggagt atttactcga ttttgagtaa gtacaaggtt
     8881 gttgttggtt ataggtatgt cattatcata aaggattttg atcactctat cataaaggta
     8941 atattacatt tatattagat aactctagtt tttattgtta tcaaagattt cttttatttt
     9001 ttcaacaaca aattagtcat tttttggatg gcagctgtca attggtctgt cgcttggtct
     9061 tgttttgctt cagatgtatt gaatagatct tcatctaccc ctgattcaag atcgatactt
     9121 tggatcattt cttcacagcc tgctacatat gaaagataag ctttttcaaa atttttatga
     9181 atgcccattg cttttgctgg tggacgcaaa gtactgactt tcttaagcat tgcccgatat
     9241 ttttttgttc cttcttcaaa taacaggatg atctcttcaa gagtcgattt gtccagttca
     9301 cttgtttttc cttcatcaat tgcgtttctg atcacttcat atttcggatg cattccttct
     9361 ccgattgctt ctgtttcctt gacgatttga tcgatggact gcacataata gccaatattc
     9421 ggtaacatgt tcgttttcct actttctcca ttgttgttat tatttattct acttgttttt
     9481 atcctcaaat gacaagtact tgaaaaaatt taatggtttc tatacagctc gccattctta
     9541 ttcgtgctca aagaaaccat ctgctaaact atattgtttc cctgcgacaa tgtcatattg
     9601 gatcaattga tattcgtcgt aaatctcttg ttgcgagcga ttgaattgcg cttctttata
     9661 ccactgtccg ttttgatagt acagttctcg ttcaaacgcc ggaagttcct gttctaatga
     9721 taaaagcagc tgataaaagc catttgttgt ctgattcgtc tgttccatta gattcgctgc
     9781 aaaataaaac ggactggtga ttgcatcgtg agtcgtttgc ttttctaatt ctcctttact
     9841 gtcgaacata agaaattgtg tttcatgcaa tgtttgtttg tcattttttg cttggatcgt
     9901 atctgaatag atccctggaa gatgatcccc ccaaaaaaca actaaagtcc ttctagaaag
     9961 attcttcaat tcttccgtga actgcttcag tgccgtgctg ctataggaaa tatcttgcag
    10021 ataattctct aagtcaagtg tcccacttcc atctgaagtt tttgcaggat agtctagatt
    10081 atcatacttt ccatcgtagg gcatatgtgt ctgcatcgtg actaaatgaa tgaactgagg
    10141 tgttttttct tccttgagaa gtgtcattac ttctttgtaa gctgactcat cagaaatata
    10201 tggattattc tctatcgtat ccgtataggt catcgtacgc tcgctgatga actggtcaaa
    10261 tcctaatgtc tgataaacat cctctctttt atacatcgaa gtattgtatg gatggatcgc
    10321 tgtcgtctgg tatgattgtg cgtttaacgc tgaaacgata gaaggaagct gatccatttt
    10381 tggtacaagc atcgtgtaag gggtggtgag ttgcgcatta aataacgcca tagaaaaacc
    10441 agtcaatgct tcaaattcga tatttgctgt tccaccgcca tagttttgag aaagcatatt
    10501 gccactatat gtttgatcag ctacttcata ataatctgcc aatggctcac cagatacttc
    10561 tacgccgttt aatcgactag gatctgagaa actttcactc atcacgaaca cgatgttcgg
    10621 cgactcttcc tctacagttt tatttttttc atcggctagc ttctggtatt tttcagtgat
    10681 ttctttgatc ttttctttcg aatatccttc aggttcatcc attggttcta ccttcagatt
    10741 gtacaaaaac ccaccgataa aaccagtgtt atagtagttc attttctggc tgtaaggtat
    10801 ccatagtgcc gttttattat atgcttttcg caacaaatta tcgggattgt tgaaattact
    10861 gatataccct agcagtccaa ttgccacaag cagtgtcgtt aatcggatca attggaatcg
    10921 ccttcctttt ttcaggcttc gaaacatgta ccagcaaaac aatcctatca cggttcctgc
    10981 ggcagctaag atcactgtaa acagcatagt gccggtcata tctttcagca atccgatttc
    11041 agtgatcatc ttcaaatcgt ctggataaat cggttcttgc cgataactca ttttcatata
    11101 atttgcaaaa cctaatacac cgatggctac gacatacgta agggaaccta aagggacaga
    11161 tcctgctagt gcaatcagaa aaataagaat aatcagcaag acgagacagg ctaagaagaa
    11221 tttttccgta tgccaagaaa aagcaaattt caacgcaagg tctacggata gatcattttg
    11281 acaccactgc aaaaataaat tactaaaaac aacaagaaaa aagatactga tgattgctgc
    11341 tgttcctttt aaaatctttt tctgtttggt cgaaagtctg cttatatatc ttttcaaatg
    11401 cttgctcctt catttctatc aataaaagaa tgttgcttct cttattttag ttgtacgaaa
    11461 ttcgaaagta aagaacggaa agaagaatta atgaaatcca aatattctct ttctttatgg
    11521 cctttctggt ttctttaata agcggacctt tcgtactcca gaaatttttt ctagctgttg
    11581 tttcacttga ttgagctgtt caaaatcagt actgtctatg tcaatcaata tgtagacatg
    11641 ctcattctta tggttacgcg atatatctgc gatcgctagc tcgttctcat tcagcagaga
    11701 aaatatctct gctaatatat tggttttctt ttgataaaat atagtgaatc gaaacggcgc
    11761 ttggaaaaac attcgtgcgg atggataatt gaccgcttct ctaaccgtac cgaacaaaag
    11821 ataatctttt aaggcttcaa ctgccaaacg tccgctgtct gccaaagctt cctgagtcga
    11881 tccccctaga tgaggcaaca taagtatacg gtcattttct aagaactctt cttctggaaa
    11941 atcagtcaag tatttagcca gatgttgttt ttccaatgct tgcataacag catatttatc
    12001 aacaatttca cttcttccta cattgataag tacagcatct tttttcattt tttctatatt
    12061 cgcctgatcg atcaatcctt ttgtatcttc tgtcaacggc agcatgacta cgatgtaatc
    12121 cgaggtagaa agaactgttt ctaaatctgc ttgccgaaca taatccaact gggcatcgcg
    12181 gatagaataa cccaagacat ccatccctaa gctatagcaa cttagcgcta cttttgtgcc
    12241 tatcgctcca agaccaagaa gaccgatggt tttgccttgc aattctcgtc ccacataagc
    12301 acttcttttg ttttctgcct gttctaaaat attaggacct gttagtgttt gtaccatacg
    12361 acttgcttcg attatcggac gggaagataa gagcaaacag cacaaaacaa gctcttttac
    12421 tgcattagca tttacacctg gtgtattcat gacgatcgtc ccgttttcag aagctttttc
    12481 aacgttgatc gtgttcaccc caacaccagc tcttgaaatt gctagcaaat ctggtgtaag
    12541 ccattcatct ttcactttcg tactacgaat aactattcct tcggctggca aatcatctat
    12601 tattttgaaa tattcccctt gtaacttttc tatctcttgt tctggaatag catcaatcaa
    12661 atgaacgtga tacataaaac cccctcctta ctaacaagta tctcgaaatt tctgcaatta
    12721 gacaactgtt taccatttga cttcctagaa gcaaaaagag cagttagagc agagtaggct
    12781 gagtgataaa aataaggcta tgacaagtta ctgccataag ccttattttc gagagcaaaa
    12841 tattgagggt gagatatctt gataaatggt gttccgttag ctgacattga taataagact
    12901 aaatttcaaa aaagttgaaa agcaaatttc gtcaatctct tcttatttct taaaactaag
    12961 tggctttttc acaacctctt tttatctaat aaccactgtt tccaactcaa ctaattttgc
    13021 ccacgtttca atttgatctg ttgttagatt tagcgagaca acggtatgat gaccgccacc
    13081 tgcctgaatc catgaatgga caccttcatg gaaattcggt ttagttttcc ataacacacg
    13141 tgcaacaggt aaattaggtg cttcagtcgt tggttcaaag gcttctactt cattgatcag
    13201 cagtttataa tgcgtaccga aatctgccat cgatacgact accccttctc ctgctttccc
    13261 atcaaataca agacgcgccg gatcttctct atctcccatt gataaaggag aaacaacgat
    13321 tcttggtttc gtcgcagcaa gcgtcggatc tacttccatc atatgggatt ccaagattgc
    13381 ttcttggcca ctagctagct cgtaagtata atcttccatg aatcctgtat ctttccctcg
    13441 agccatgatt ttcagcaacc gatcaatcgc tgcagttttc cagtcgcctt ccccagcaaa
    13501 accgtatcct tctgccatca aacgttgtac agctagtcca ggtagttgtt tcattccata
    13561 caggtcttca aaattagtcg taaaagcgtt atagcctcct tcttctaaga aacggcgaat
    13621 accgatttcc tgctgagctt gtacttttac atgttcctcc catgtctttt cttcataatc
    13681 accatagtcg aaggtataca attccttgta ctcttcaaat aattgatcga cttcttcgtc
    13741 tgatacgcga tcgattactt gtacaagatc tccgatacca aaataatcaa cagtccaacc
    13801 aaattgaatc tgtgcttcca ctttgtctcc ttctgtgaca cccacgtttc tcatgttgtc
    13861 accaaaacga gccactttta ttccaaaact ttcattatag gctactgcaa catccatcca
    13921 atcagcaatc tctttttgga tctcaggtcg tttccagtag ccaacgacga ttttattttg
    13981 tttatttaat cgagcattga tgaacccata ttcgcggtca ccatgagcgg attgatttaa
    14041 attcatgaaa tccatatcga tcgttttcca aggaatgctt tcgttatatt gcgtagctaa
    14101 atgaagcaat ggtttttgta gaagctttgt cccgcggatc cacatttttg ctggtgagaa
    14161 agtatgcatc caagtgatca cacctgctac ttcttctcga taattgacct ctttcatgat
    14221 ttttgtgatc gtatcagcag taactgctaa ttcttgaagg cggattggat aaggcaattg
    14281 gccattttca ttcagttcat cgaccatttc ttgtgcatgt ttctttactt ctcgcagtgc
    14341 ttcttcccca tataaatgct gtgatcctac aacaaaccaa aattcttttt ctccaatatt
    14401 caacataatt agccactcct tttatttgtt ttgtccgtaa tacgcatttt ttccgtgttt
    14461 tcgtaaataa tgtttatcta aaatccgttg aggcagctct tctgaaaatg aattgagctg
    14521 tctggtaaat aaattcatct tgcatacttc atctagtacg actgcgttca ttaccgcact
    14581 ttgcgcatct ttcccccaag taaatggacc gtgtccgtgt aaaagtaccc ctggaattgc
    14641 taagggatca attttgcgtt ctttgaatgt ttctatgata acttttcctg tttcatattc
    14701 gtacccgctg tcgatttcct gttgtgtcaa aaatcgagca caaggtaccg atccataaaa
    14761 agtatctgca tgcgtcgtcc ccatcgcagg aacatccaat cctgcttgtg cccagatcgt
    14821 cgcccatgtc gagtgagtat ggacgattcc tccgatttga ggaaatgaac gatataaaac
    14881 agcatgagta ggcgtatccg aagagggatt gagtttacct tcaatgatat gattgtccaa
    14941 gtccacaacg accatgtcgt ctgctgtcat ctcttcataa ctgactccgc taggtttgat
    15001 aacgaaatat cgtgtttcag gatcaaaagc gctcacattt ccccatgtat atttgacgag
    15061 tccgtgttta ggcaagtcaa gatttgcttg gtatacttct tctttcagtt gttccagcat
    15121 tttctatcac tcctttttct tgcaattgat gaatcgctgt tttttcaatg tcgatcccct
    15181 cacggtaacg tctcataaat atctgatagc ctgctatttc ttctgctgaa ggtgtgatgg
    15241 tgattccttg gtcttgtcca aacacgtctt ctgccaagaa gttttctaaa gacttcccat
    15301 gtcgatccat catataggct gctaataacg cgattcccca cgctccacct tctccggctg
    15361 tgtccatcac tgtaactggt gcctccatag cagatgctag aatccgctgt ccaacttctg
    15421 gtgttttaaa aatcccccca tgccccacta aacgatcaat ttggacattc tcagatttca
    15481 atatatccat tccgattctc attgcaccaa aagcactggc taaatggatc cgcatgaaat
    15541 tcgctaaatc aaaacggcta cctggtgtac gtacaaataa tggccttcct tcatttactc
    15601 ctgtaatgtt ttcaccggaa aaataaccat aagaaagtaa tcccccgcaa tccgtatcgc
    15661 cttctagcgc tttgttgaat aataccgtga ataatttatc tctgcttatc tccataccca
    15721 aagaattggt aaattcttca aaaatactga cccaagcatt gatatccgaa gagcagttat
    15781 tcgtatgaac catgcctact agttctcctg aaggtgtagt gaccatgtcg atttccggat
    15841 ggacattttc tagttctttt tctaatacga tcatagcgaa ggcggaagta ccagccgaaa
    15901 tgttccctgt ccgtttggct acactgttcg tagcgaccat accggttcct gcatcccctt
    15961 ctggcggaca aaccgggatt cctggtctca aatttcccgt agggtcgatc agataagccc
    16021 ctgattcgct taacttgcca gcttcttctc ctgctactag aactttaggc aaaatgtgat
    16081 ccaagtcaat cgctacgtgc gctttttcga ttaactgacc aaaaatattt ttcatttctt
    16141 gatggtaggt tttcgttgaa ggatcgatag gaaacatgcc tgatgcatct cctacgccta
    16201 gtactttctc accagtaagc tgccaatgga cataacctgc aagcgttgtc aaaaaattaa
    16261 tttctttcac atgttcttct ttattcaaaa tagattgata caaatgagcg atactccatc
    16321 tctgcggaat attgtattga aatttttccg ttaatattcg ttctgcttct tcagtgattg
    16381 tatttcgcca agtacgaaat ggtactagta actgttcctt gctatcaaat gctagataac
    16441 cgtgcatcat tgcactgaac ccaatcgacc cgattgtcgt aagattctcg ccatagattt
    16501 cgaatacttc tgctgccatt tttcggtaac tgacgcgcag tcctttccaa atatcttcta
    16561 atgaataggt ccagatacca ttttctagtt tgttttccca ctcataacta ccagcggcaa
    16621 taggagtaaa agatgaatca atcaatacag ctttgatcct agttgatcca aactcaatcc
    16681 ctaaagatgt acgaccatct atgacatcta acatactttg cgcatttact tccttcataa
    16741 tgatttaact ccctccttct cgtcaaacct gatcaaaccg tctttttagt agcgcttaca
    16801 atgattattg tatacttttg tacgtacatg tcaagcggtt atttacaaga atacaataaa
    16861 aaacacttgt tttgtacgta cataagttat aaaatgaaat tgcttacaaa atctgatcaa
    16921 ccgccttctc gaaattctag aaggagagaa tacttatgaa aaaagaaaaa aaagtttcct
    16981 catcgtttat ttattttttt ggcgcatttg gcggcattct atttggatat gatattggtg
    17041 ttatgaccgg cgccttacct tttttacagc atgattgggg tttggcaggt aaagcttctt
    17101 tgataggatg gatcacgtct tcggtcatgt taggtgcgat ccttggcggc tctttatctg
    17161 gtcttctttc tgataagctt ggacgacgaa aaatgattct tctttctgca cttattttta
    17221 tggctggttc tgtcctttct gcatctgccc ctcacaatgg ttcttatttt ctgattgcag
    17281 caaggattct tctaggttta gctgttggcg ctgcatctgc cctagttcct gcttatatgt
    17341 ctgaaatggc tcctgctaga ctaaggggaa gattgtcagg gatcaatcaa gtgatgattg
    17401 cttctggcat gctcctttcc tatgttgcag attatttgct aaaagtgttg cctgaaacga
    17461 tggcttggcg cgtgatgtta gggctcgctg ctgtccccgc gctgatttta tttttcggtg
    17521 ttttagctct accagagtca ccgcgttttc ttatgcaatc tggccgcttg gaagaggcaa
    17581 aaagagtatt gaactatatt cggacaccaa aagaagctga acaggaattc gaacaaatcc
    17641 agctgaatgt caaacaagaa aagacaaccg gaacttcttg gcatactttg tttttagaaa
    17701 aatatcgttc cttagtcatt gccggtattg gtgtagcagt attccagcaa tttcaaggag
    17761 ctaatgctat tttttactat attccgctga tcgtagaaaa agcgactggg catgcagcga
    17821 gcgatgcatt gatgtggccg atcatccaag gaatcatttt agttgctggt tctttgctct
    17881 ttttggttat tgcagacaag ttcaatcggc gaactttatt gaaaataggc ggtagcgtga
    17941 tgggactttc attcatccta ccagctgttt taggaactgt actggatgct catacaaatt
    18001 cgttgttgat tctcttgttt ttatgtattt atgtcgcatt ttattcttgc acatgggctc
    18061 cactgacatg ggtaatcata ggcgaaatat tcccactagc tgtccgcggg cgagcatctg
    18121 gcctagcctc ttcttttaat tggattggtt ctttcctagt tggcttgctt tttcctgtga
    18181 tgactgcttc tatgtctcaa gaaatcgttt tcggcatttt tgggatcatc tgctttttag
    18241 gggttttatt tattcaagag atcgtaccag aaactcgtgg gaaatcttta gaggaaatcg
    18301 aacaaagtgc atctaaaaaa acttatccta aacgtatttc tatccatcat tcttaaagag
    18361 gttgtgacag aagtggtcag cttcaagaaa taagaaggaa ccgcttagtt tccagtcaca
    18421 aggaacaatt tcaaaaaaca gcttctcatg tttgatgaat attgtgactt gtgaccagtt
    18481 ggtaggagaa caccgtttag tggagaggtg gtgaaagaaa cgttcagcct tgagcgatga
    18541 caaaagctct cacaaacatt gcgacgagta accgtaggag caataagagg cggtaaaaac
    18601 ccgtctctta tttttcgttt tctttcactg tttcccgttc aactagtaca ggttcataga
    18661 gaatatttgg ctgtgcctgt cctttttcaa tcgttgtgac gatccaccgt gcggcatctt
    18721 gtcccaactt ttcttttgga tgcgtcaatg ttgtcaattt tttattccct aaatggctta
    18781 aagaagaatc gtctgttcct acgatcgata ggtcatctgg aatatgatag ccattttctg
    18841 tcaacttatc tagcattaaa ctagctattt gatcgttata gcaaatgatg ccatctaaag
    18901 cttgttcttc tttcaaccgt tgtactgttt tttcagcgat tgtttctcgt gtttccgtgg
    18961 tgaaagtaat gatatcttcc gtcaaaaaac gcacattata ctgttcacac gcgctgataa
    19021 aacctttcat cctgtatttt ccttgaagat cgtcgatttt tgtgatgaac aacagacgct
    19081 gatgtccttt ttttatcaaa tattctgctg ccataaaacc gactttcaca tcatctaaac
    19141 aaatataaga ggcatttaac tcttcataag cagcattgat catcaccatc ggaatgcctt
    19201 gttctctcaa agcaacataa agtgctaagt ttgggttata ctgattactt ttcgtaggct
    19261 caacgatcaa accatctacc ccataagcaa tcattttctt caaacactct ttctccaatt
    19321 cgtgatcgtt attcgtactg gctaaaagca atgaataccc agattctcgt aattcatttt
    19381 cgattcctcg gataatcgac ggaaaaatat aatcagagag atatgtggtg atgacaccga
    19441 tcgtttttgt tctgttcaat ggtgtttttt ctttcttata gctgtcatct acatatgtgc
    19501 ccgacccttt ttctttacgc agatagccct catttaccaa cacagcgatt gcttgccgaa
    19561 tcgtatgtct gctgacgtca tatatttctt gcagcttgaa ttctgaggga atggttgtat
    19621 ttacttcgta ttcctcacga aggatctttt gttttatctc ttctgcaata tcttgatatt
    19681 ttgccatttg ccttgcccct ttttatttgt acgtacatat atcataacga gtctgcaagg
    19741 tttattcaag caaaaatcaa ggtttcttgt gctcataagg catacggaac aggttcacca
    19801 aattctggaa atccttgctc attccacgta aagcattgcg cgttcgcgtg ccgattagga
    19861 ttgtctaacg gatccccttt ttggttcttc tctggtcgag catgatagat caaaatatca
    19921 aagcacccgt cttctgaaac cgtaaacgag ttgtgaccag gaccaaattg cttatttttt
    19981 tcagaagaaa cgaatactgg ctccgataat ttatgccatg attcttttaa taaaagattc
    20041 ttgtgctcat cagcccacag caggcccatg gcatagtttt catctgttgc actaccagaa
    20101 tacgtaatga atattttccc attgcgagta aggactgctg gaccttcatt tactgaaaaa
    20161 ccccgttttt cccattcata ctcaggtata gaaaggagga tctgcttccc ctttaacgtc
    20221 caaggattat ccatttcgga tagataaaga ttagaattcc cagtaatttc aggatcttgt
    20281 tgggcccaca cataatatag ctttccttga gaagagaaag ttgttccgtc taaactgaaa
    20341 gtttcccttt tcgtttttat ttgtcccttt tctatccaat ctgtattcat tggatctata
    20401 tgctgatttt caagtacgaa catgcggtga tgatgagatt catcacgtat agttttatga
    20461 ttgcttgcag caaagtaaat ataccatttt ccattgatat aatggacttc aggtgcccag
    20521 atcaattcac tcatcggtcc ttcttcatga gcctgccata caatcaggct tgcagcatgt
    20581 tctaattcat tcagtgactt tgcccttctt aattcaatca cttggtagcc tggaaccgat
    20641 cctgtaaaat aataataacc atctgtatgt ttatataccc aaggatctgc ccgttgccga
    20701 acgataggat tattataaag tgtcttcatc ataacaaatt tcctcacatt ttctttattc
    20761 taattacatt ccaagagtat ttttctatcc ctacatttaa ttcgtcatgt ttttctattg
    20821 aaaaatagtt tatttcaggt tttatttttt ctggttcttc acgtgtattt ttgtccaaca
    20881 gatcgctact ggttaatgtg tagtgctctc cttgatctag caaattgaaa tcatgttcaa
    20941 tcgttatctt ttgttgttca tttgtgcgat tgatcaagaa aatagtcagc tgattgccgt
    21001 tatctacgat cacactgtcc acataaggaa cattttttat cttttcacaa tcataaacag
    21061 ggcaaatgat ttgttgtttc aatacttgac ctttagcata tttcgataag tgttgcatca
    21121 cataataagt tggattcctc catgcttgtc ctttagcatc tgtcagtatt aaaggtattg
    21181 tgtttactaa caatgattga caactaatct ttacgcgatc tgcgtgtcgt aaaatagcca
    21241 gccctaatga accaaataat aatgtatctt ccatagtaaa gtaacaccaa tcgatgggac
    21301 taccgacttc aaaatcccta tgtctttttt ccggttgggc atgtacattc cattcatcaa
    21361 acgctagata cattgttttc tcacttctca aatatccttt tacataatca caagtagcaa
    21421 cgatttcttc aatttgacga tcgaatcgat aagcttttgc aaggtatgtc gctgtatcat
    21481 cacgctcatt tttaggaggt ttagtcaagt cttcttcatc atacttatca atatagttat
    21541 gcaaagctaa ataatctaca ttctcataag cttcttctaa tgcgatacga tcccattcag
    21601 ggtacgatgg taaacgtggc gtagagcttc caactaagat tgtttcaatt gaatcatcca
    21661 ctaacttcat gacttttgag gcttcatttg ctaacctacc atattcataa gcactcttag
    21721 cccccacttg ccatggaccg tctagctcat tccctaaaca ccataattta acgccaaaag
    21781 gctttttttg cccatttttt attcttaaat cactccagaa ggttcctttt ggaaaattac
    21841 aatattctac taaatttttt gcttcatcaa ttccttttgt tcctaaattc accgtgaata
    21901 tcggtttcgc ttgtacatga ctgatccatt taaaaaattc gtggagtcca aaagaatttg
    21961 gttctatacc tcgccatgct aaatcaattt tttttggtcg ttgttctttc ctacctacag
    22021 tatccaccca atcataacca gatgtaaagt ttcctccagg atagcgaatg acacctaatt
    22081 ctaattcctt tacataagag aggacatctt gtctaaaccc gtcaccatca gctgtttcat
    22141 ggcccggttc ataaatccct gtatagacga cacttcccat atgctcaaca aatgagccaa
    22201 acagccgaga actgatctct cctaccgtaa aatcctctct gatttttact ttcacttctt
    22261 cgtccaacag ctttccctac ctttcatttc ctatagtcaa gataatagat acaagtttcc
    22321 atacgccacc agcaaagata aaaataaaag gagggaaaag aaatatgagc agtacaaatc
    22381 ctaataacaa tacgcaacaa agaatatttc tcaaaaaatc aaaaaatact ttccatgcac
    22441 tttgttcaaa aatttgtaaa aatgtgatat ctttgttttc agaaatcaca tatatccagg
    22501 ataaaagaaa catggaataa ataagtaaaa ctacatatcc actaattgaa agtacgattg
    22561 tctgttcttc taataattga ttaacaagaa aataaataat gatcgttaac agactaagga
    22621 acaagttctt accaaaatgt tgctttattc cttgaaagaa aaaataaatg atatttcctg
    22681 atccctcacc tttccattgc gccattacat aaaaaacagc acatgtagca ggcacgatag
    22741 taataacagg caaacacacg attacccaga gaatacctaa taagatgtaa tcagaaacca
    22801 caatcaactg tccataaaat ttagtctcaa ctattttcat cttcctcact ctttcattcc
    22861 agacatagct gttccttgaa caaaatattt ctgtgcaaaa atataaacca ctagtaatgg
    22921 cagcattgaa ataaccgttc cagccattac aggtccataa aaagaaatgt tctgccctgt
    22981 aaataaagct aaccctgctg gtaaagttct catttttgaa ctacttgtca atatcaatgg
    23041 ataaagcaag tcattccaac tattcatcag cgtgaaaatc cctaatgata aaagagtagg
    23101 tttgctcaat ggcaacatga ttttcaaaaa aatattaaac tcacttaatc catcaatgcg
    23161 tgctgcctct tccaaagaat ctggtaaagt aataaaaaag gaacgcatca tgaagatacc
    23221 aaatgcagtt gtcatacgag gaatgatcaa acctgcataa gtatcaagca tgccaagcaa
    23281 attgacttca ataaatagag gaatcatgaa gacttggaat gggatcatca ttgtcagcaa
    23341 aacgaaatag aacaaaactg attttccttt gaaacgcata cgagcaaatg cataccccgc
    23401 aagagaatca aagaatacag aagtgatcac tgtccctcca gcaaaaatca ctgtattttt
    23461 gatgtaatct aataaaggga tcgttgtcca aaccttaata tagttatcta gggtatagct
    23521 atctgcaaat attctaggcg gaaatgagat aatgtctttt tcgtgtttaa aagaagaaat
    23581 aatcagccac aaaaaaggaa acgcaataac caacaatata actagcatac taatcgttcg
    23641 aatgattttt ttaccactaa ctgcttttcg actattcaca gtaactgtaa tcgagctttc
    23701 tggtgccata caggtttccc tcctatttca tttgtttttc tttccgtgac atgtacacat
    23761 tagtggagat agctatagtt gcaacaatga taaacaaaac cgaagacagt gctgatgcat
    23821 agcccaattc ataaggtgct gtaaatcctc gactgtaaat atattgaaca gctgtttctg
    23881 tcttaaattg tgggccacca gcggttgcaa catatacttg gtcaaacact tgcatcgaac
    23941 caatcagatt cgttaaaata caaaaaccta aggagggaac gatctgaggt agcgtaatgt
    24001 taaaaaactg ctgtcgattt gttgcaccat ccatttgagc agattcatac aaagagggag
    24061 agattccctg aatagcagtc aagagaatcg tcatagtaac accaaaattc ttccaaacag
    24121 tcatgatggc taccgtcggc atagcaaggg ttggatcgcg aaaaaattga gggatcggca
    24181 agccgacttg tgaaaacaag taaggaatcc cccctacatt cggatcaagc aacatagaaa
    24241 aaataatcgc aattgacgtc aatgaacaaa taactggaat gtaaaatgtc gaacgacaaa
    24301 aacgattgaa tctggtattt ttagataatg ctactgctgc taataaacca acgataattt
    24361 gtgttggcgt ttcgagtacg gtgaaatata aagaatgaat caacgaatta actgctcgtt
    24421 catcactaaa tatacgtgtg aaattttcaa aacttacaaa atttcttgat gtaaagaaaa
    24481 tattcaaatc tgtaaaacta atagcaaatg ttcctaaaag cggtatgaca gtaaataata
    24541 ataaaactaa aaaagatgga gttaaaaaca aatacgcagc acgatcagga cgattccaat
    24601 atttggtgaa tccgttcata tagtccacct catctttcat tttgataaaa tatacaatct
    24661 tggtttaaac gaatctgaat aagatatccg aacaatcgct gtgatttttg caataacacc
    24721 tatctgcaaa tactaggtcg atcattcaga tatctgtatc ttgccaacag tctatcctgt
    24781 ataaagttct gtactatttt tgcagtaatt gattgatggc atcatctgca tcgttcatga
    24841 gtgtctcagg atcttctcct gctaataatt tttcaatcag tggattgatg atatcgttat
    24901 tgatcgcaga gatttgggta aaacctggta aatatggctc tgcatagtcc atttgtttcg
    24961 ataattcaga tacaatagga tcatttttta cttcttcatc ttttgctact gaatccaagt
    25021 aggcaggaaa accattttcg atactccatt ttttaccaat ttcagttgta ttccaatatt
    25081 tcaaaaactc atagacagct tcttttttat tattatctgt acttgccgga atggcatatc
    25141 ctacgccatc taaaatagct gtttttttgt ctgaatcact cccttgtggt aaggtagtca
    25201 cattataatc aatctcgttt gctttcaatc cgttatttaa ccatggccca ttgatttcaa
    25261 tggctagttg accagcattc attaaattgt ccatttcggc gccagaaatc gattctggac
    25321 cagtcttgtc tacatggatc attttttgga tcgcttttaa cgttttaagg ttctcaggag
    25381 acgcaaattc agacttagta taatcatcgt tcactaattt accgccattt gctaacatcc
    25441 agttatataa cggtgcagtt ccatccttat tgaagacaaa acctgataca tttttactac
    25501 tatcagttaa ttttggtgcc atttctgtca attcttccca agtagtaggt ggttgttcag
    25561 tatctaatcc agcttgttta aacaaatcat tattccaata catatacatg ccttgtacct
    25621 gcatggggac aaaatactgt ttaccttcta cttcacctaa ttcaatagcc gtttctttaa
    25681 aatcagattt atctactcca tcatattccc agaaatcgtc taatggttgt accgctccgt
    25741 tttctacata ctgtgcaaaa tctggataat ttaatgcaat aaagtcaggt gcttttttag
    25801 aagaaatcgc tgtaggaagt ttttcattca aattggccca agtcatgata tccatttcaa
    25861 ttttgatgtt tttatcattt gttttattaa attcattaac gatttcttgc aatatttcac
    25921 catcagatgc agtaaaccca ttccaaaaag tcaaagtcac ttctttatca gaatcagatt
    25981 ctgctgaact tccgccaccg caagctccta aaatacctgt tgcagctacc aacaaagagc
    26041 caattaacca ttttttacct ttcatttttt gttcctcctt catctgataa acgacatctg
    26101 accacattcc aagatagcgg tttcaataaa caattgattt tcccatcttc agcttttaca
    26161 tttgttgaat gacttggttt tacttgttca cttttaggaa gattagttgc ttttgtatca
    26221 aatccactca tttcagaaaa atcaatcact ccctctaatg ataattcaga caagtgaaca
    26281 gttagattca tttcttcttc tccacggtta actgcaaata tgacaagttc tttgtcattt
    26341 attacggcta tactttctaa atacggcact tcttcaaaat cttccgtcgc atagtttgct
    26401 gaacgaattt gtgggcttaa cacctctcct tgtccataca cagcagcttg cataaatgga
    26461 taatagatcg tctgtctcca agcttctccg tttgtttctg tcctgattgg tgcaataacg
    26521 tttaccaatt gagcaagaca agcaatcttt actctatctg catgtttcaa taaagtgatt
    26581 aatagacagc ctaatagtaa tgcatcttca aaattatagt tatcttctag taaaggagga
    26641 gcaatctgcc aaggcgctgc ttgctcatct tgttccgctg aatgatacca aatattccat
    26701 tcatcaaagg acaggttgat ctgcttctta ctatgtttct tcgccttgac tgtatcgcac
    26761 atcgctgtca cttcagaaat gaaccgatcc atgtctaagg aacgtgcaag ataatgagaa
    26821 agatcatttt tttggttgcc ataatattga tgcagagaca aataatctat ttgatcgtac
    26881 gcttgatcaa gaactgttaa ctcccaatca ccaaatgtag gcatttgccg attcgaactt
    26941 ccgcataaaa ccacctcaat tgaatcatct acgagtttca tcgccttagc cgtttcctct
    27001 gctaaccgtc catattcttc cgctgtttta tgtccaatct gccaaggacc gtccatttca
    27061 ttccctaaac accatgtctt aattccaaaa gggctctctt gaccattcga acgccttaaa
    27121 tcactccaat aagttccttt aggaaaatta caatattcga ctaaattacg agctgcatct
    27181 actccacgag tacccagatt gacagccata ttgatttcgg cccccacatg ctgacaccag
    27241 ttataaaatt catgaatccc aacttcattc gtttcaattg tccgccaagc taaatccaac
    27301 cttctaggtc tatttgctat gggcccaata ctgtcttccc aacgaaatcc tgacacaaaa
    27361 tttccaccag gatatctaat catgggaatg ttcaattctt ttaccaagtg aatgacgtct
    27421 tggcgaaatc cacttttatt agctgatgga tgatctggct gataaatccc ttcatatact
    27481 gctcgtccca tatgttcaat aaatgaactg tacaaccgcg gatcaacttt gcctacacgc
    27541 ttgacactac taatcaacat tgtggcttcc ataactgacc tcctttaatt tatacatgaa
    27601 taatacacct cgttgaaagc gttgtcaata atataaatgt taaacttact tattttttat
    27661 cgattttaat taaccaaaat attaataatg attaaaacaa ataatttaat ataactttta
    27721 ttaacaaata tgttaatata taagaaaaac cttttaagag gtgatatata tgcaactaca
    27781 aataaacgaa gaatccttac cggtatatga agcattagct agtaaaacaa ggatcaagat
    27841 catccagcta ctttccaaaa aaaagatgaa tgtcaaagat ttggcaaaag aacttggagt
    27901 gagcagtgct atcacgacga tgcacgtgaa aaagctagag gaagcaaaca tcatcaaaac
    27961 agaaaaagta ggacaacaaa aaatttcgag ccttcgtgtc gataaaatcg atattagttt
    28021 tccagaaaaa atattcaatg cttttgatac gaaagaaaca tcgataccta ttggtcacta
    28081 tacaaattat gcaattgaac caacatgcgg tttagctacg atccatgatt ttatcgggaa
    28141 agtggatgaa ccacgctatt ttatggatcc tcgaaggatg gatgcccgca ttctctggtt
    28201 taccagcgga tttgtcgagt atcaggcacc taatttttta aatacagagg atacattgga
    28261 gatgttggaa gtttctgtgg aaatttcttc tgaatttcct ttttcaaatg acaattggcc
    28321 ttcagatatc actttcagcc taaatggtgt ggagctagga acatggacga gccctggaga
    28381 ttttgctgat atccgcggta aatatacgcc tgattggtat ccagataatc ttaatcaata
    28441 cggcttgctg aagacgatcc gaattaccaa acacttgacg aatatgaatg gagaaccctt
    28501 gtcaaacatc acgatcaacg acatccctaa agagcaagac acttggcatt tgcgaatcga
    28561 agtgaaagac gatgccaaac atgtgggggg ctgtacttta tttggtaaag gattcggtaa
    28621 ttacgatcag gatatcaaat taaaagttta ctacagctga tatttttctt ttctatttta
    28681 taaaacaaaa acagccaatc cctatgaagg gacggctgtt tttgctttat tgttatttcc
    28741 agaaatcatc caaaacggaa attggcaaat gtcgtttatg ttgtgtcttg ttccaccatg
    28801 cttcgatttt ttcttgtgct tgagcgggaa cttcttttcc ttctagataa tcatcaattt
    28861 cattatatgt gacacctagt gcgacttcat ctgcaatcat tggtttccca tcttcaagat
    28921 ctgcagtcgg aatttttgtg tataattttt ccggtgcatt caatgcttga agcaattgtt
    28981 tcccttgtcg tttgtccaaa cggaataatg gcaaaatgtc ggctccacca tcaccaaatt
    29041 tcgtgaagaa tccagtaatg ttttctgcag catgatctgt ccctaatacg gctcctgctc
    29101 tttctccagc tacggcatat tgcgtaatca tacgttgacg tgctttgata tttcctttgt
    29161 taaaatcgct gattcccaca cctgcttcgc ttaatacttg tacttgtgca tcaacagctg
    29221 gtttgatatt tactcgcaga ctaacatcgg gctggataaa gtcaagagca gcttttgcat
    29281 cttcttcatc tgcttgttca ccataaggaa gacgaacagc gataaattga tagttttcat
    29341 cttttgtttc ttcacgcatt tcttccatcg ttagttgcgc caaacggcct gctaaagtag
    29401 aatcttggcc gccgctgata cctagtacaa aagttttaag aaacggatat tttttcaaat
    29461 atgctttcat aaagtcaata cttttacgta tttcttcttt tggatcaatt gttggtttga
    29521 cacctaattc attgataatt tctgtctgca ggctcattta ttctcctcct atgcttctgt
    29581 atgaagttct gctacgcttt ttttcattcg ttccatgata gccattttat ggttccagca
    29641 ttcagtagac agatcgactg ggtatttttg cggattcaag tctcgtttat actcttccca
    29701 tagtgaatct agattttctt ttgtatattc tttgatttcg tttagtgcag gtaattcgta
    29761 aactcttttt ccttcgataa agatatcttg taaaactggg cgagctgtaa agtctcgaac
    29821 agttttattg ataaacgtat gcactggatg gaacatgaag atagcttctt cttctcgagg
    29881 gtcttcatcc cataatgtca cgtagtctcc ttcagatttc ccatcaaaat ttcgagtgat
    29941 gcgccacact tgttttttcc ctggagtagt tactttttct gcattgcttg ataacttgat
    30001 cgtatccatc atttttcctt catcatcttc gatcgatact agcttgaata ctgcaccaag
    30061 ggctggctga tcataggctg tgatcaattt tgtcccaaca ccccatacat caattttagc
    30121 tttttgcatc tttaaattca aaatcgttgc ttcatccaag tcattcgaag catagatttt
    30181 tgcttccgta aatccggctg catctaattg ttcccgaaca cgcttagata tataggccat
    30241 atctccactg tcgatgcgta cgccttggaa gttgattttg tcacctaatt ctttcgccac
    30301 gcgaatcgca cttggcacac ctgatttcaa cgtatcataa gtgtcgacta agaacacgca
    30361 gtctttatgg gttttagcgt aggccataaa tgcttcataa tcatttccat aagattggac
    30421 taacgaatga gcatgggtcc cgctagctgg tatcccaaat atcttcccag cacgtacatt
    30481 gcttgttgca tctgctccgc cgatatatgc cgctctagtt ccccagactg ctgcatccag
    30541 ttcttgtgcc cgtctagttc caaattccaa tagtggatct tctccgatga cagatttgat
    30601 acgagctgct tttgtcgcga tcaacgtttg gaaattgacc atattcaaca gtgctgtttc
    30661 tactaattgg cagtgcgcta aaggcccttc cacttggatc aatggttcat ttgcaaaaac
    30721 tagttctcct tcacgagcag aacgaactgt tgctttaaat tcaaaatttt gtaaatactc
    30781 caaaaaacct tcaggataga cttctaatcc acgcaagtaa gcaatatcgg aatcattgaa
    30841 cgtcagattc tctaaataat tgactaaccg ttccaatcct gcaaaaaccg cgtatccgtg
    30901 gttaaaaggc atttctcgaa agtaacattc aaaaacagca tgtaaatcag ctcgacctaa
    30961 ttcccagtat gtctgcatca tattgatttg atatagatcc gtatgcagcg tcaaactgtc
    31021 gtctgggtaa gtattattca tcttgtatta ctcctcttgg caagttttca tttcctaccc
    31081 attataacaa aaaaaattaa taggaaaaga aatcttccat tttttctgaa aaagcacatg
    31141 ttttacggat atttcttgct aaaaatgaac tccatctgct aaaactatcc tttcttattg
    31201 gtatttaacc taaacgagaa agaatactta ttttattcaa tgtatccgag acacgaagaa
    31261 attagcgttt tctatttttc ttacttgata aaataagtag attttcctac tacaatcgta
    31321 gccgttaaga taccgatgat tgaacaaata taaccaaatg tctctacaac ataatcaatg
    31381 gcaaaagttt ttgaagcaat aatacgtctt tccttttttg cttcttatcc ccaaagaaac
    31441 ggatataact tattatgtat tgaatttttt tggaaaggcg aattatgaca agatatacct
    31501 tgcgttgaaa aaactcgcgg aaaaaatatc acacgttttt aaaaagatac aaagtaagaa
    31561 agcagctgtc aatagaatgg aaacaaaaat ggcgagacat caatcagtct cgccttgttt
    31621 t
//
LOCUS       DACEEZ010000024        31547 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20313_2017
            SAMN12639966-rid9879223.denovo.024, whole genome shotgun sequence.
ACCESSION   DACEEZ010000024 DACEEZ010000000
VERSION     DACEEZ010000024.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639966
            Sequence Read Archive: SRR10023158
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 31547)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 31547)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741683.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741683.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:44:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,016
            CDSs (total)                      :: 2,962
            Genes (coding)                    :: 2,889
            CDSs (with protein)               :: 2,889
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 36 of 73
            Pseudo Genes (incomplete)         :: 29 of 73
            Pseudo Genes (internal stop)      :: 18 of 73
            Pseudo Genes (multiple problems)  :: 8 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31547
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639966-rid9879223.denovo.024"
                     /isolate="E20313_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(76..543)
                     /locus_tag="IWO89_001169"
     CDS             complement(76..543)
                     /locus_tag="IWO89_001169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA starvation/stationary phase protection
                     protein"
                     /protein_id="HAQ0455939.1"
                     /translation="MKFQQTKEVLNQLVADLSQMSVVVHQTHWYMRGPGFLTLHPMMD
                     EFMDDLNAQLDEVSERLITLDGSPYSTLREFADNTKIPDEIGRWDRTIPERLETLVAG
                     YRYLADLYQKGIEVSGEEGDDPTQDIFIEFKTATEKRIWMIQAHLGKAPEIDA"
     gene            complement(663..2201)
                     /locus_tag="IWO89_001170"
     CDS             complement(663..2201)
                     /locus_tag="IWO89_001170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconokinase"
                     /protein_id="HAQ0455940.1"
                     /translation="MNVSIGLDIGTTQTKAVAFTDEAQEAASAYFRYPLIQETQGMAE
                     QDQELIFQGVCTVIKEVIKQLPSDTIIQFVSFSSAMHGLILLDEEKTPLSRMITWADN
                     RAADEAEKLKYTVLGKEFYRQTGTPIHPMTPLNKIKWLQNSQPELFKKTAYFVGIKDY
                     IFYRLFGELISDYGTASGTGYFDIHEFQWAEEVLDYLNIRIEQLPQVFPSTYQVTGLP
                     SKTAAELGLPNDISFVLGGADGPLSNLGLGAFGDTIAALTVGTSGALRFITTQPQFHP
                     EMETFCYVLDQKHWVVGGATSNGAGIFDWAGHTFLQEVVQSAKEKGENPYDALLSAIA
                     FVPAGANGLIFHPYLLGERAPLWDADAFGSFIGLQRQHTEKEMMKAILEGICLNLYRI
                     LKGVCQENQLTEIRATGGFAQSPIFRQMMADVLGYPLVFPTVTEASALGAVILGWRSI
                     GKIQSISEAQELIELKEQVGVQETDHKKYQKIYPLFVSTQKHLAQTYQQFAQLREELA
                     DSEK"
     gene            2281..2967
                     /locus_tag="IWO89_001171"
     CDS             2281..2967
                     /locus_tag="IWO89_001171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase"
                     /protein_id="HAQ0455941.1"
                     /translation="MLLYFIIGCCIGSFLCLTAQRIPLGHSIIYPRSHCVKCCRSLSW
                     YELIPILSIIVQRFRCRYCRCRLPFYYLLAEALCGGLFAWFFTFSSARNFSTFIWMLS
                     ALLFSLMDLFYFMVHSHTLFCSWAILWIFWLQAGVFQWQSVLLTFIVGGVCLRYGQSY
                     LGAGDTLLMLSWSGGLSLEELLQILFLASLFGISVFSVYFICHKKKLEKLPFIPFLSS
                     ALLIVLHLRY"
     gene            complement(3168..3695)
                     /gene="msrA"
                     /locus_tag="IWO89_001172"
     CDS             complement(3168..3695)
                     /gene="msrA"
                     /locus_tag="IWO89_001172"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HAQ0455942.1"
                     /translation="MTKKAVFAGGCFWCMVKPFEEQPGIISVTSGYTGGHVPNPTYEQ
                     VTTGTTGHTEAVEIEYDPEVISYDQLVEIYWQQTDPTDALGQFADRGDSYRPVIYYND
                     EEERNIAERSKQALQDSGRFDKPIVTKIEPRSEFYPAEDYHQDYYKKNKLHYNLYREG
                     SGRAGFLRRHWGVKK"
     gene            complement(3779..4456)
                     /gene="pgmB"
                     /locus_tag="IWO89_001173"
     CDS             complement(3779..4456)
                     /gene="pgmB"
                     /locus_tag="IWO89_001173"
                     /EC_number="5.4.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-phosphoglucomutase"
                     /protein_id="HAQ0455943.1"
                     /translation="MKIKAALFDLDGVLVDTARYHYEAWLVLANQLSIPFTEKENEQL
                     KGISRTESLERLLSFGKMEQKFSEKEKSAFAEQKNNLYLQAIQKMDETSVLPGAIAVL
                     EYLKKTNIKIGLGSASKNARLILEKTNLTSYFDVLIDGTQVSKAKPDPEVFLKGAQQL
                     NVPPNACLVIEDSEAGCQAALAGNMHVLGIGENINLPSAEYVIPDLTVFDQVRSFWHL
                     SEAVSRI"
     gene            complement(4453..5583)
                     /locus_tag="IWO89_001174"
     CDS             complement(4453..5583)
                     /locus_tag="IWO89_001174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HAQ0455944.1"
                     /translation="MRILIAPDSFKESLTALEAAQAIQSGFQEVFVQAEYDLLPIGDG
                     GEGTVSSLADSLRLKRQTAVVTNAFGKNKEIQYASDGQTAIFEMAEIVGLKDVPLNQQ
                     QPLRLSTRGVGELIRQLLQQGVRHIIIGVGGSSTNDGGIGMAEGLGYAFYDQDNQKVE
                     ALGENLGKIATVDKTNVPIELIKAKITVITDVTNLLCGKEGASFVFGPQKGLKENELA
                     VADQAMHDFYMRFAPQVIEASGSSAGGGMGAGLLAFASGKIVSGIDYVLDLLEFDKRV
                     QQADLVIVGEGRLDGQSLKGKAPVGIARRVPEGVPVIAICGSVGKGSEKAADVGISAV
                     FPILPAAVPLQEALQQATDNLHRTAKNIASLLQVVHFQGGNR"
     gene            complement(5583..7766)
                     /locus_tag="IWO89_001175"
     CDS             complement(5583..7766)
                     /locus_tag="IWO89_001175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 65 protein"
                     /protein_id="HAQ0455945.1"
                     /translation="MKVEEKQFDLEYINKYASQMTIGNGFFGIRGSQEEDYRNQVRGM
                     FAAGVYNRPLGAESAELVNLPDVIRWEIKIDGEHFSLETMKVLAYERALDLEMGELKR
                     YALIETAQGKKVEVITCRVADQNNLHQAALKVVIKPLTEDCQVDVKTGIDGQQTNFGT
                     QQLVEKELRVFGDDLISASYQTTESKIDIGIAAKFNKAGVFQAKNRRITASFSEKIRK
                     NTSFTLEKIIVLSTSLQQTDPFKKSIAQAQKSVSYTEILEQSMSYWTDFWHQHRITIQ
                     GDSSFDQLAMDFAAYHLQIMTPRNDPSVSVGAKGLTGEGYKGHVFWDTEIFILPFFLY
                     QFPEVAKNLLLYRYDRLEGARQKARDYHYQGALFPWESAHTGQEETPKFAALNIKTGT
                     RQEVASAAAEHHISADLAYAVQEYVQATEDQSFLETKGKTLIHETAAFWLSRAVKVDD
                     HLEIHRVIGPDEYTEYIDNNAYTNYLAHYNVALAVKYQAGDPLFLKECQNFLDKLYLP
                     VPNKEQLIPQDDTYLSKPVIDLTTYKERPGSQSILLDYARSEVNELQVSKQADLVMLL
                     YLLPEYFSQPIVSKNLHYYENKTIHDSSLSKAIHAIEACRIQEMEWAYHLFQEACLID
                     LGPDPHSSDDGIHAASLGALWLAVIFGFSGITVTNGTLSISPRLPSAWEKISFPFIWK
                     NHRLLFVLTKKQIVIKKETKETLPIFVNGDQYQLEKELILERRGK"
     gene            complement(7763..8788)
                     /locus_tag="IWO89_001176"
     CDS             complement(7763..8788)
                     /locus_tag="IWO89_001176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HAQ0455946.1"
                     /translation="MKKVAIIGAGQVAEKVHAAYYQKRSDVLELAAVVDPVSQRAEDF
                     CRRTGFSHAYTSVTQMLNEISPDIVSVCTPNRFHFEHVLAALEANASVMCEKPPAMTA
                     DQARKMAETADKNGQILAYGFQHRFSDEATMIKNHLDVLGDIYYVEANALRRSGVPGW
                     GTFIDKELQGGGPLIDYGIHMLDTAMYITGFPKVKSVRAYSFQKIGTKKNSGKFGKWD
                     PSKFTVEDAIFGTLELENGKIIQLNTSFALNIREDKLNVAFCGDQAGANLYPGELFED
                     VDGKLVSLQKTTPVNDPHELSMAHFVNRALGDQTQMIADGWQGYVIQNLVENLYRSAE
                     TGEVITL"
     gene            complement(8833..9660)
                     /locus_tag="IWO89_001177"
     CDS             complement(8833..9660)
                     /locus_tag="IWO89_001177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HAQ0455947.1"
                     /translation="MKFGTQDQAFFPRDILEKFRFIKDCGFEEYEIDGKLLIENIDTV
                     KQAIKETGIPVVTACNGYDGWIGDFIEERRLNGVRQIAEILRALSEVGGKGIVIPAAW
                     GMFTYRLPPMVSPRSKEGDYKAVSESLRYLDKVAEETGTVIYLEPLNRYQDHMINLLA
                     DARHYIDSNQLKHVQIIADFYHMNIEEDDISKALTTQKEYVGHIHLADNHRYQPGSGA
                     IDFTRHFQTLLQNEYEGYMVYECRLRGENEVKIYQESLQYLKNCLETARHLNDLEKE"
     gene            complement(9657..10724)
                     /locus_tag="IWO89_001178"
     CDS             complement(9657..10724)
                     /locus_tag="IWO89_001178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding alcohol dehydrogenase"
                     /protein_id="HAQ0455948.1"
                     /translation="MKKLVALEPRIAGFKDYEDREIEKNEVRIRVIFATPKPGTEIVD
                     FRGLSPFIDEEFSEEWRLFLPREEGSQRGVVFGEFQLGNMIVGTIEETGSLVTEYQKG
                     ELVCTYGPIRETLITNGVDNYKLRKLDRLDQWKNAVCYDPAQFALGGIREAHVRAGDA
                     VAIFGLGAIGQIAIQLAKKAGATTIVGIDPIAQRRELAEKHGADYTLDPMKADVGFRL
                     KELTDKRGVDSVIETSGSAAALQASLIGIGYGGIISYVAFAKPFPAGFNLGREAHFNN
                     AKIVFARASSEPNPDYPRWNRKRIEDTCWELLMNGYLDCEEIIDPVVPFAESAEAYMR
                     YVDQHPEESIKMGVQVRGGEQ"
     gene            complement(10750..11586)
                     /locus_tag="IWO89_001179"
     CDS             complement(10750..11586)
                     /locus_tag="IWO89_001179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HAQ0455949.1"
                     /translation="MKNKKSRKILFYATILLLLAVNLFPFLIMLMTSFKDSQEAIATT
                     QTIFPKEWTFEHYQDIFNAEIFPFIMYFKNSLVISLIASVTAVVIGISGAYALSKLRF
                     KARSKINASFYTVYMFSGILIVVPLFKIISGLGLYDTNTALIITMVVQTLPTAIFMLK
                     SYFDTIPTDIEEAAMIDGLSRIQIIIRIIIPLAISGIISVFVYCFMVSWNDYLFASIF
                     LSTSEKFTLPIGLNTLFSTPDYIWGRMMAASLVTALPVIIMYALSERFIKGDLVEGGV
                     KG"
     gene            complement(11583..12452)
                     /locus_tag="IWO89_001180"
     CDS             complement(11583..12452)
                     /locus_tag="IWO89_001180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAQ0455950.1"
                     /translation="MNRPRKHSDLKLAMGLLAPSMLLLAVLVLIPMLSNIQISFFDMP
                     LNPNSDAVFIGLSNYLEILSDSEFYRSLGLTVAYTFLVVAGSTALGLAVAILLNRKFR
                     FRKTVRSLILLSYVTPSISLIFAWKYMFNNSYGIVNDLLVDRLQLFNEAPLWFDQPVA
                     SFFMVVLFAIWRYYPYAFISFLAILQTVDKTLYEAAEMDGANAWQKFKIVTLPAIKPV
                     LATVIVLRSIWMFYMFTDVYLLTNKVNILGVYLYQTAFAFNDLGKAAAISILLFIIIA
                     AVVLLSRKKVDLK"
     gene            complement(12522..13805)
                     /locus_tag="IWO89_001181"
     CDS             complement(12522..13805)
                     /locus_tag="IWO89_001181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HAQ0455951.1"
                     /translation="MKKIIVTTCLLATGFLLTACGESQSSNESQQTTIQFMHSSVEQE
                     RLSVINELVADFEKENPDIKIKQVPVEESAFNTKIVTLARSGKLPEVMEVSQDFAKVM
                     DKDELIDQTAVQNVIEEAGEDNYYDGAKNLVRSEDGKSYIAAPISGWVQGIWYDKEKL
                     SEAGFSEPENWDDILKIAQHFTDKENKQYGIAMPTAEGTMSEQAFSQFALSNGANVLD
                     DEGEVTIDTEKMREALSFYQNLSQYTMPGSNDVTEIKDAFMNGTAPMAIYSTYILPSV
                     YEAGNSENIGFAIPTQKDQAVYGTVSGLTISSGLDEKQKEAAEAFTAFLSQPKNMEKW
                     VLMSPGGAQPVNKQVVELDGYKENEVIKSFGELPSEIASAFDEVQVFGLVGEKNFTKM
                     GDITSSGVIGKAVNQVTVGNEDVDQALADAQKNTK"
     gene            complement(13819..15504)
                     /locus_tag="IWO89_001182"
     CDS             complement(13819..15504)
                     /locus_tag="IWO89_001182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphorylase"
                     /protein_id="HAQ0455952.1"
                     /translation="MYTKLLQAIYRDSDLSEKTASMIEQKVSVYQQKKGQAGKLHWDE
                     EDIFLITYGDQFFEEQQPTLRTFKKFYQKYLADTFRVVHFLPFFPYTSDDGFSVVNYQ
                     AINDRLGTWEDIEEMRSETRLMFDFVCNHMSAESTWFQEYLNLNDTYKDFFIDLPLDT
                     DTSSVTRPRTSPLLTTFIDKNGKEKHVWTTFSEDQVDLNFKNPEVLLKMIDILLFYLE
                     KGASFIRLDAVGFLWKELGTSCIHLPETHKIIQLFRAIADEAAPGTVLITETNVPHKD
                     NISYFGNGENEAQMVYQFSLPPLTLQAIRSGNTSKLKEWLKTIDFASKKTTFFNFLAS
                     HDGIGLNPVRGILPEKTILELVEQLKKEGARVNYKKNPDGTTSPYEINATYLDALSSP
                     TDTTEWRSKRFLAAHSFLVTLPGVPAIYIQSVLGSRNDYKGVEKTGENRKINRQKFLL
                     ADIEKELQTDPLRTAVYGELTQMLSIRKKEKAFHPNSLIYLLDSADSFISFIREAKEE
                     KVLVIHNFSNQSAGYLLPKGDYLDLFSGQIIHCSQERKLIFQPYEYMWLKIIK"
     gene            15779..16804
                     /locus_tag="IWO89_001183"
     CDS             15779..16804
                     /locus_tag="IWO89_001183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family transcriptional regulator"
                     /protein_id="HAQ0455953.1"
                     /translation="MKPTIYDIAKKANVSKSTVSRVLNNQPNISDKAKASVLQAIKEL
                     NYEPSKIARGLSGNGFDAILALSHRTSHTTYGNPFFSDVIQSISAVSEQNNFDLILQT
                     AKTPEEELEKCAKKIQEKMIRGIIILSSTMDETLLFELDQYNIPIVVIGKVGNDYTNI
                     YSVDTDNFQDSYDLVETLIKNGHRHIGCLHAPTEVYVSNDRLNGYRQCLFDHHLDIRN
                     EWIIDGGFRLEDSMAAMNDLMKLRERPTAIFSTDALKSLSIYKVAEQSGKQIPDDLSI
                     AGFSDPLLSSLFSPQLTRIDIPTNQLGIKATELLFQLIHHQYPEEKRIILSTEITLTD
                     SIQQQEM"
     gene            complement(17399..21052)
                     /gene="rpoC"
                     /locus_tag="IWO89_001184"
     CDS             complement(17399..21052)
                     /gene="rpoC"
                     /locus_tag="IWO89_001184"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HAQ0455954.1"
                     /translation="MIDVNKFESMQIGLASPEKIRSWSYGEVKKPETINYRTLKPERE
                     GLFCERIFGPTKDWECACGKYKRIRYKGIVCDRCGVEVTRSKVRRERMGHIELAAPVS
                     HIWYFKGIPSRMGLVLDMSPRALEEIIYFASYVVIEPGNTNLEKKQLLTEREYREKRE
                     QYGQEFQAAMGAEAIKQLLDNVDLDAEAAELKEELKSAQGQKRTRAIRRLDILEAFRA
                     SGNQPSWMVMDVIPVIPPDLRPMVQLEGGRFATSDLNDLYRRVINRNNRLKRLLDLNA
                     PGIIVQNEKRMLQEAVDALIDNGRRGRPVTGPGNRPLKSLSHMLKGKQGRFRQNLLGK
                     RVDYSGRSVIVVGPFLKMYQCGLPKEMAIELFKPFVMRELVQRELASNIKNAKRKIER
                     QEDEVWDVLEDVIKEHPVLLNRAPTLHRLGIQAFEPVLVQGRAIRLHPLVCEAYNADF
                     DGDQMAVHVPLNEEAQAEARMLMLAAQNILNPKDGKPVVTPSQDMVLGNYYLTMEEEG
                     REGEGMVFRDQNEAVIAWRNGYVHLHSRIGVNPNSLGEKPFTEWQKERTMITTVGKII
                     FNEIMPPEFPYLNEPTDFNLTVQTPDKYFVEAGTDIPAHIKEQELVLPFKKKNLGNII
                     AEVFKRFKVTETSKMLDRMKDLGYKHSTHAGITVGIADISVLNEKQEIIENAHKQVET
                     ITKQFRRGLITDDERYERVIAVWNAAKDSIQQKLMEGLEAKNPIFMMSDSGARGNISN
                     FTQLAGMRGLMAAPNGRIMELPIISNFREGLSVLEMFISTHGARKGMTDTALKTADSG
                     YLTRRLVDVAQDVIIREEDCGTDRGLEIQAIREGNEIIESLEDRLVGRYTRKEVRHPE
                     TNELIIAGNQLISEDIAKQIVDAGVETVTIRSVFTCNTKHGVCKHCYGRNLATGSDVE
                     VGEAVGTIAAQSIGEPGTQLTMRTFHTGGVAGDDITQGLPRVQEIFEARNPKGQAVIT
                     EVTGDVIDISEDPATRTKEVTIKGKTDTRTYTVPYTARMKVAEGDMIHRGAPLTEGSI
                     DPKQLLQVRDVLSVENYLLREVQRVYRMQGVEIGDKHIEVMVRQMLRKVRVMDPGDTD
                     ILPGTLLDIADFKDQNYNTLVSGGVPATSRPVLLGITKASLETNSFLSAASFQETTRV
                     LTDAAIRGKKDPLLGLKENVIIGKIIPAGTGMQRYRDMEPKEVGVASENVYSISDIEA
                     QMAAEDAMNNQTK"
     gene            complement(21131..24748)
                     /gene="rpoB"
                     /locus_tag="IWO89_001185"
     CDS             complement(21131..24748)
                     /gene="rpoB"
                     /locus_tag="IWO89_001185"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HAQ0455955.1"
                     /translation="MAGHVVKYGKHRERRSFARISEVLELPNLIEIQTDSYQWFLDEG
                     LREMFEDILPIDDFNGNLSLEFVDYELKEPKYTVAEARAHDANYSAPLHVTLRLTNRE
                     TGEIKAQEVFFGDFPLMTEQGTFIINGAERVIVSQLVRSPGVYFHGKVDKNGKEGFGS
                     TVIPNRGAWLEMETDAKDISYVRIDRTRKIPLTVLVRALGFGSDDTIFEIFGDSETLR
                     NTVEKDLHKNASDSRTEEGLKDVYERLRPGEPKTADSSRNLLNARFFDPKRYDLANVG
                     RYKVNKKLDLKTRLLNLTLAETLVDPETGEIIVEKGTVLTHQVMETLAPFIDNGLNSV
                     TYYPSEDGVVTDPMTVQVIKVFSPRDPEREVNVIGNGYPEAAVKTVRPADIIASMSYF
                     LNLMEGIGNVDDIDHLGNRRIRSVGELLQNQFRIGLARMERVVRERMSIQDTETLTPQ
                     QLINIRPVVASIKEFFGSSQLSQFMDQTNPLGELTHKRRLSALGPGGLTRDRAGYEVR
                     DVHYSHYGRMCPIETPEGPNIGLINSLSSYAKVNKFGFIETPYRRVDRETGRVTDQID
                     YLTADIEDHYIVAQANSPLNEDGTFAQDVVMARAQSENLEVSIDKVDYMDVSPKQVVA
                     VATACIPFLENDDSNRALMGANMQRQAVPLINPQAPWVGTGMEYKSAHDSGAALLCKH
                     DGVVEYVDASEIRVRRDNGALDKYDVTKFRRSNSGTSYNQRPIVHLGEKVEKGDTLAD
                     GPSMEQGEMALGQNVLVGFMTWEGYNYEDAIIMSRRLVKDDVYTSIHIEEYESEARDT
                     KLGPEEITREIPNVGEDALKDLDEMGIIRIGAEVQDGDLLVGKVTPKGVTELSAEERL
                     LHAIFGEKAREVRDTSLRVPHGGGGIVHDVKIFTREAGDELSPGVNMLVRVYIVQKRK
                     IHEGDKMAGRHGNKGVVSRIMPEEDMPFLPDGTPIDIMLNPLGVPSRMNIGQVLELHL
                     GMAARQLGIHVATPVFDGASDEDVWETVREAGMASDAKTVLYDGRTGEPFDGRVSVGV
                     MYMIKLAHMVDDKLHARSIGPYSLVTQQPLGGKAQFGGQRFGEMEVWALEAYGAAYTL
                     QEILTYKSDDVVGRVKTYEAIVKGEPIPKPGVPESFRVLVKELQSLGLDMRVLDIEET
                     EIELRDMDDEDDDLITVDALTKFAEQQTAKELEKKAAEQVEDEKDDVIQNFETAEDNL
                     D"
     gene            25476..26444
                     /locus_tag="IWO89_001186"
     CDS             25476..26444
                     /locus_tag="IWO89_001186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HAQ0455956.1"
                     /translation="MKKNIIIGLGAALLLALGGCAQKDNSAEASDGQKTIGVLQLVQH
                     NSLDAAYKGFKEGLAEEGYKEGDNLTIDYQNAQNNQDNLKSMSEKLVKEDPDLLLGIA
                     TPAAQSLLNETTTIPMTVTAVTDLEQAHLVTSNEKPGGNVTGTTDMVPIEKQIGLLLN
                     IVPDAKTIGIMYNNGEANSKIQADLAEKELKKAGVKVKVLTANSTNDVQQVTKSLAKD
                     VDGIYIPTDNTFASAASVVGEVAKETKTPVVAGAVDQVKDGGLATVGIDYESLGKQTG
                     IMAAKILDGKNPSELPVETANDLELYVNKDMAKALGINPDSIKEAE"
     gene            26462..27367
                     /locus_tag="IWO89_001187"
     CDS             26462..27367
                     /locus_tag="IWO89_001187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAQ0455957.1"
                     /translation="MTNLLSLFLSSASQGVLWALLAIGVYVTFRILDIADLTAEGSFP
                     LGAGIAAVSITNGYHPLTACLLGFLGGAAAGLVSGLLHTKLKIPALLAGIITMTGLYS
                     VTSRVMGAPNISLLGQPTIFHWVESFGITKDNAVLIVGLLFALIVVCLLVLFLRTETG
                     LAIRATGDNLAMSEANGINTDNMKIIGYMISNGCIALSGSLLAQNNGFADLNSGIGTI
                     VIGLASIIIAEVLLRNQPLFLRLLTVVLGAVIYRFILALVFELNVEPSDSKLASAFVL
                     VICLSFPQIQQKFHLPQKKKGGSVK"
     gene            27364..28170
                     /locus_tag="IWO89_001188"
     CDS             27364..28170
                     /locus_tag="IWO89_001188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAQ0455958.1"
                     /translation="MNDVLTLENLHHTFEKGTINENHVLKGINLSLDPGDFVTIIGGN
                     GAGKSTLLNSIAGTFSVDQGQILLNGKNITKKSVVERSKSISRVFQDPKLGTAVRLTV
                     EENLALAMKRGKKRGFFRGVKPQDRTFFKDQLVRLNLGLENRLSSEIGLLSGGQRQAI
                     TLLMATLQRPELILLDEHTAALDPATSKTVMELTEKLITEQNLTAFMVTHDMEDAIRY
                     GSRLIMLHQGRIVVDIQQEEKQQLTVPQLLALFKQNSGTALKDDQVLLSS"
     gene            28327..29295
                     /locus_tag="IWO89_001189"
     CDS             28327..29295
                     /locus_tag="IWO89_001189"
                     /EC_number="6.3.4.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin--[acetyl-CoA-carboxylase] ligase"
                     /protein_id="HAQ0455959.1"
                     /translation="MTTKDKVLQLLKKTPDFLSGEKLAQQLEVSRTSIWKAIKELEKA
                     GYRFEHGPTGYRYLPSDVLDAKEIQQELRSFIPDLKVQTMLTSESTMKDAKLAAAQGD
                     SVSKLFVADTQEQARGRFGRPFFAEPGRGIYMSLLLHPDKRFDELPQYTIIAASACVK
                     AIESVTGRSASIKWVNDIYMDGKKVCGILSEAVSDMESGRISHIIIGIGLNFSVPQNH
                     FPADLQEKAISVFPDGQASVTRNQLISKIWQELFTYLSHPEKALDFYKEKSFVLGKTV
                     SFNQKGTTYVGLAKEIGDHGELIVQTNEQKMSLSSGEISLTSIRTQ"
     gene            complement(29612..30136)
                     /locus_tag="IWO89_001190"
     CDS             complement(29612..30136)
                     /locus_tag="IWO89_001190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HAQ0455960.1"
                     /translation="MDYLDMLCESVDRAYDRFERLFEGVSIEEANRFPVEDRAPQIKS
                     MTWLAWHTARELDFQIADLAGTSPIWHSQDWESRFPMTIADNEQDWKHSLEQAKQIQA
                     NQIEDLLGYLRGAADFAISYLRRLEPSELDEVIDENWTPPVTRGMRLVSIIDDAAMHS
                     GQVIYARRLLGLRD"
     gene            30423..>31547
                     /gene="scm"
                     /locus_tag="IWO89_001191"
     CDS             30423..>31547
                     /gene="scm"
                     /locus_tag="IWO89_001191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="collagen-binding MSCRAMM adhesin Scm"
                     /protein_id="HAQ0455961.1"
                     /translation="MKKRASLLVLVALLFNIFSYGVAVSAETYIPKDPGNIYNDLTGN
                     YGLLGIASQFHVFAKNKTTVNAHTDGNIATHELDAKNNFGTELHEGLLNQEINYIQKT
                     DSFANSSGIPTGSTRMNKFVVGSNVDVALQDGHPTVNGSRLDHLTPDDFYKDRAGHEY
                     IDFEQEFAKLNVAANDLATITPAKTYTAADFPDMNNRTIDLTDLSDTGFLLVNIDAEV
                     LTMNTPLQIINPNDQVVVFNVINSSSALNVQSPIKYNDRSNHETEDFSDANISWNFGN
                     EMTDLTISAPFQGTILAPNATIRITQNQDGTIIGTNVILDAATNRWDPNEIFISDNGT
                     DTTDTSDSTDTSTTSDSSDSSTSSDSTDTTSTTSDSTDTSA"
BASE COUNT         8856 a         6815 c         5938 g         9938 t
ORIGIN      
        1 acgtttgttt ctctaatact caggtcaaaa cgctgatttc acaacctctt tttcggtcaa
       61 gttcaacaac agtttttacg cgtcgatctc tggcgctttg cccaaatgag cttggatcat
      121 ccagatacgt ttttctgtgg ctgttttgaa ttcgataaag atatcttgtg ttggatcgtc
      181 gccttcttct ccagaaactt cgattccttt ttggtaaaga tctgctagat aacgatagcc
      241 tgcaacaagt gtttccaaac gttctggaat agtacgatcc cagcggccaa tttcatcagg
      301 gatttttgtg ttatctgcaa attcacgcaa tgtagaataa ggtgaaccgt ctaatgtgat
      361 caagcgttcg gatacttcat ctaactgagc gttcaaatca tccatgaact catccatcat
      421 tgggtgcaat gtaaggaaac ctggtcctct catgtaccaa tgtgtttggt gaacaacaac
      481 agacatttgg cttagatctg caactaattg atttaatact tctttggttt gttggaattt
      541 catctttctt gcctcctaac attttcttac acttaaaata atagtagact ttcaatgaaa
      601 agtacaacaa aatcatttac ctgagaacga ttttcagttc gcttttttat ccgtaaagcc
      661 aatcattttt cagaatcagc taattcttct cgcagttgag caaattgctg ataagtctgt
      721 gctaaatgct tttgtgtcga aacaaataat ggatagattt tctgatattt tttatgatca
      781 gtttcttgta ctccaacttg ctctttcaat tctatcaatt cctgtgcttc actgatactt
      841 tggatctttc cgatgcttct ccagcctaaa atcacagctc ctaatgcgga tgcttctgtg
      901 acagtaggga aaacaagcgg gtaacccagt acatcagcca tcatctgacg aaaaatcgga
      961 gattgagcaa acccgcctgt agcacggatc tctgtgagct gattttcctg gcaaactcct
     1021 tttaaaatcc gataaaggtt gagacagatc ccttctagaa tcgccttcat catttctttt
     1081 tctgtatgtt gccgctgtaa accgataaaa ctgccaaacg catctgcgtc ccaaagcggt
     1141 gctcgctcac caagtaaata gggatgaaaa atcagaccat tggcacccgc tggcacaaaa
     1201 gcgatagccg aaagcagtgc atcatatgga ttttctcctt tttctttagc agattgaacg
     1261 acttcctgta aaaatgtatg ccctgcccaa tcaaagatgc cagcaccatt gctagtcgct
     1321 cctcccacta cccaatgttt ctgatccaga acataacaaa acgtctccat ttctggatgg
     1381 aactgaggct gagtagtaat aaaacgaagt gctccgctcg ttcctacggt caaagcggcg
     1441 atcgtatcac caaaagcacc taaccctaga ttagaaagcg gcccgtctgc ccctcccaaa
     1501 acaaatgata tatcatttgg caaacctagt tctgctgctg tttttgatgg tagacctgtt
     1561 acttgatagg tggaaggaaa gacttgcggc aattgctcta ttcgaatgtt caagtagtca
     1621 agcacttctt ctgcccattg gaattcatgg atatcaaaat atccagttcc tgaagctgtt
     1681 ccgtagtcgc tgatcagttc gccaaacaat ctataaaaga tatagtcttt gataccgaca
     1741 aaataagcag tttttttgaa taattcaggt tggctgtttt gcagccactt gattttgttc
     1801 aatggtgtca ttggatgaat cggcgtccca gtctgccgat aaaattcttt gccaagcacg
     1861 gtatatttca gtttttctgc ttcgtccgct gctcggttat ctgcccaagt gatcattcga
     1921 gataacggtg ttttctcttc atccaacaat atcaggccat gcatcgcaga ggagaaggag
     1981 acaaattgaa tgattgtgtc tgaaggcaac tgtttaatta cttcttttat aacggtgcag
     2041 acaccttgaa agatcaattc ctgatcttgt tctgccatcc cttgtgtttc ttggatcaaa
     2101 gggtagcgaa aataagcgga tgctgcttct tgggcttcat ctgtgaaagc aactgctttg
     2161 gtttgtgtgg ttccgatatc taatccgatt gaaacgttca ttttttcacc tcaaatttta
     2221 tgtatgtaca aaaatttagt ttaagcttgc gtatcttggt tttgaaaagg gggagatggc
     2281 gtgttacttt attttatcat tggttgctgt atcggatcat ttttatgttt aaccgcccag
     2341 cggattccgt taggtcactc tattatttat cctagatctc attgtgtcaa atgctgtcgt
     2401 tcgctttctt ggtatgaatt gattccgatt ctctcgatca tcgtccaacg atttcgctgc
     2461 cgctattgca ggtgtcggtt gcccttctac tatttgttgg cagaagcctt atgtggtggt
     2521 ctttttgcct ggttctttac tttttcttcc gcaagaaatt tttcgacgtt catatggatg
     2581 ctttcggctt tgctattttc gttgatggat ttattttact tcatggtcca ttctcacacg
     2641 ctattctgtt cttgggcaat tttatggatt ttttggttgc aggccggcgt cttccaatgg
     2701 caaagtgtgc tgttaacgtt tatcgtaggc ggtgtgtgcc ttcgctatgg tcaatcctat
     2761 ctcggagctg gcgatactct gttgatgctt tcttggagtg gtggcttgtc tctagaagag
     2821 ttgcttcaga tcttgtttct tgctagtctg tttggaatat ccgttttttc tgtttacttt
     2881 atctgtcata aaaaaaagtt ggaaaagctg cctttcattc cttttttgag cagtgcgttg
     2941 ctgattgtcc ttcatcttag gtattgaaaa aaggttgtga caaacgactt gtcacaacct
     3001 ctgatatata gtgttctctt accaaatcct tgtgacttgg agctaggcga caggttcaca
     3061 acctctccac taaatggtgt tcaatcagct gaccctcagt attagctcaa aatgctggtt
     3121 ttacaacctc tgatatacgg tgttccataa gtagcttttt cggcttctta tttcttgacg
     3181 ccccaatgtc gacgaaggaa acctgcacgt ccagaacctt ctcgataaag attgtaatgg
     3241 agcttgtttt tcttgtaata atcctgatgg taatcttctg caggataaaa ttcgcttctt
     3301 ggttcgattt ttgtcacgat tggcttatcg aatcgcccgc tgtcttgcaa tgcttgtttt
     3361 gaacgctctg caatatttcg ttcttcttca tcattatagt agatcactgg acgataagaa
     3421 tcaccgcgat cagcgaactg tcctaatgcg tcagtcggat cggtttgctg ccaatagatt
     3481 tccaccaact gatcataaga aataacttct ggatcatatt cgatctctac tgcttctgta
     3541 tgccctgttg tgcctgttgt tacttgttca taagttggat ttggtacatg cccgccagta
     3601 taaccggatg tcacagaaat gatccctggc tgctcttcaa atggtttgac catacaccaa
     3661 aagcatccgc ctgcgaaaac tgcttttttt gtcatataaa atcccccttt tttctcatca
     3721 taccttgatt tccaaaagga ggataacgtt ttgcttgtga cagaaaaaac ggaaactatt
     3781 atatacgtga tacggcttct gataaatgcc aaaacgaacg tacttgatca aaaacggtta
     3841 aatcaggaat cacatattca gcagaaggaa gatttatgtt ttcacctatt cccaatacat
     3901 gcatgttacc agccaaagct gcttgacaac cagcttctga atcttcaatg accaagcatg
     3961 catttggcgg tacattcaat tgctgtgctc ctttgagaaa aacttctgga tcaggttttg
     4021 cttttgatac ttgcgtgccg tcaataagga catcaaaata acttgtcaaa ttcgtttttt
     4081 ctaaaatgag gcgtgcattt ttgcttgctg atcccaatcc gatcttgatg tttgtttttt
     4141 tcaagtactc aagaacagct attgcaccag gcaaaacgga tgtttcatcc attttttgaa
     4201 tcgcttgaag atatagattg tttttttgct cagcaaaggc tgatttctct ttttctgaga
     4261 atttctgttc catttttcca aatgacagca atcgctctag tgactcggtt ctggatattc
     4321 ctttcagttg ctcgttttct ttctcagtga atggaatgga cagctggtta gctaaaacca
     4381 gccacgcttc gtaatgataa cgagctgtgt ctacaagaac gccatctaaa tcgaatagtg
     4441 ctgctttgat tttcatcgat tacctccttg aaaatgaacg acttgaagta aagatgcgat
     4501 gtttttagct gtgcgatgaa gattatcagt ggcttgctgt aaagcttctt gtaaaggcac
     4561 agctgcagga aggatcggaa aaacggccga aatgccaaca tctgctgctt tttcgcttcc
     4621 tttaccaaca cttccgcaaa tagctatcac aggaacgccc tctggtactc ttctagcaat
     4681 cccaactggc gcttttcctt ttagactttg tccatctaga cgtccttcgc ctacgattac
     4741 gaggtctgct tgctgtaccc gtttatcgaa ctccagtaaa tccaatacgt agtcgatgcc
     4801 agagactatt ttccctgaag caaatgctaa cagtcctgca cccattccac caccagcact
     4861 actgcccgat gcctcgatta cttgaggggc aaatctcata taaaaatcat gcatggcttg
     4921 gtcagccact gctaattcat tttctttcaa gcctttttgt ggtccgaaaa caaaagaagc
     4981 gccttctttt ccgcataaaa gattagtaac atcagtaatg acagtgattt tggctttaat
     5041 cagctcaata ggcacgttcg ttttatcaac tgtagcaatt ttacctagat tttctcccag
     5101 tgcttctact ttttgattat cttgatcgta aaaagcataa cctagacctt ctgccatgcc
     5161 gatgccgcca tcgttggtac tacttccgcc aacgccgatg atgatgtgtc gaacaccttg
     5221 ttgcaacaat tgtcgtatca gttctcctac tcctcgcgtg gataacctaa gtggctgttg
     5281 ttggtttagt gggacatctt tcaatccgac gatttctgcc atttcaaaaa tcgcagtttg
     5341 accgtctgaa gcgtactgga tttctttgtt tttgccaaat gcattagtaa cgactgctgt
     5401 ttgccgcttc aaccttaaac tgtctgctaa tgatgaaacc gttccttctc ctccatcgcc
     5461 tataggtagc agatcatatt cagcctgaac aaaaacttct tgaaaacctg attgaatggc
     5521 ttgtgctgcc tctaaggctg tcagactttc tttgaaagaa tctggcgcaa tcagaatacg
     5581 cattattttc ccctccgttc caagatcagt tctttttcca gctggtattg gtctccattg
     5641 acaaaaatag gtaatgtttc tttcgtctct ttttttatta ctatttgttt ttttgtcaaa
     5701 acaaatagca acctgtgatt tttccaaata aaaggaaaag aaattttttc ccatgctgaa
     5761 ggaagtcttg gagaaataga aagagttcca ttcgttactg taatacctga aaaaccaaat
     5821 ataactgcta gccatagtgc tcctaatgaa gcagcatgaa ttccgtcatc tgatgaatgc
     5881 ggatctggtc ctaaatcaat caggcacgct tcttggaata aatgatacgc ccattccatt
     5941 tcttggatac ggcaagcctc tatggcgtga atcgctttac tcaacgatga gtcgtggatt
     6001 gttttgtttt catagtaatg caaattctta gaaacgattg gttgagaaaa atactcaggt
     6061 aataagtata acagcatgac aagatctgct tgcttgctga cttggagttc atttacttcg
     6121 ctacgggcat aatcaagtaa aatagactgg ctgccaggac gttctttgta agtggtcaaa
     6181 tcaataactg gcttactgag atacgtgtca tcttgcggaa tcagctgttc tttgttaggc
     6241 actggcaaat ataacttatc taaaaaattt tgacattctt tcaaaaaaag cggatcgcca
     6301 gcttgatatt taacagctaa ggccacatta taatgcgcca gatagttcgt atatgcatta
     6361 ttgtcgatat attctgtgta ttcatctgga ccaatgactc tgtgtatctc taaatgatca
     6421 tcgactttta cagctctgct cagccaaaaa gctgctgttt catgaatgag cgtttttccc
     6481 tttgtttcta aaaacgattg atcctctgtt gcttggacat actcttgaac ggcataagca
     6541 aggtctgcac tgatatgatg ttctgcggca gctgaagcaa cttcttgtct tgttccggtt
     6601 ttgatattca atgcagcaaa tttgggtgtc tcttcctgac cagtgtgtgc gctttcccat
     6661 gggaaaagtg caccttgata gtgataatct cttgcttttt gccgtgcgcc ttctaatcga
     6721 tcataacggt acagcagcaa attctttgct acttcaggaa actgatataa aaagaaaggc
     6781 aggataaaaa tttccgtatc ccagaaaaca tgccctttat aaccttcgcc agttagtccc
     6841 ttcgccccta cactaacact tggatcattg cggggagtca taatctgtaa atggtaagca
     6901 gcgaagtcca tcgctaactg atcgaaagaa gaatcgcctt ggatcgttat ccgatgctga
     6961 tgccaaaaat ctgtccaata agacatcgac tgctctaaaa tttctgtata actaacgctt
     7021 ttctgggctt gggcaatact tttcttaaac gggtctgttt gctgaagaga ggtagagagc
     7081 acgatgatct tctccagcgt aaaggacgta ttttttctga tcttttctga aaaggaagcg
     7141 gtgatccggc gattttttgc ttggaataca cctgctttat tgaatttagc tgcaatccca
     7201 atatcaattt tactttctgt tgtctggtag gatgcactga tcaaatcgtc accaaatact
     7261 cgcagttctt tttcaaccaa ctgctgtgta ccaaaattgg tttgctgtcc atcgataccc
     7321 gttttcacat ctacttggca gtcttcggtc aacggcttga tgaccacttt cagggctgcc
     7381 tgatggagat tattttgatc agcaacacga caagtaatga cttccacttt ttttccttga
     7441 gcagtttcaa tgagcgcata gcgtttaagc tctcccattt ctaaatctag tgcccgctcg
     7501 tatgcaagaa ctttcatcgt ctcaagagaa aaatgttcac cgtcgatttt tatttcccat
     7561 ctgatgacat ctgggagatt cactaactcg gctgattctg ctcctaaagg tcggttatat
     7621 acaccagctg caaacattcc ccttacttga ttccgatagt cttcctcctg gcttcctctg
     7681 ataccaaaaa atccatttcc aatggtcatc tgactcgcat acttattgat atactctaaa
     7741 tcaaactgtt tttcttctac tttcataagg taataacttc tcctgtttct gccgaacgat
     7801 ataaattttc caccagattc tggatgacat atccttgcca gccatctgca atcatctgag
     7861 tctggtcacc gagcgcccga ttcacaaaat gagccatact tagttcatgt gggtcattaa
     7921 ctggcgtggt cttttgtaag gaaaccagct ttccatctac atcttcgaat agttcgcctg
     7981 gatacagatt agcacctgcc tgatcgccac aaaaagccac attcagtttg tcttctctga
     8041 tattcaaagc aaacgaagta ttcagttgga tgatcttgcc gttttctaat tccagtgtgc
     8101 caaaaattgc atcttctact gtgaatttgc ttggatccca tttcccgaat ttccctgaat
     8161 tttttttcgt accaattttt tggaagctgt acgcccgaac gctttttact ttaggaaatc
     8221 cagtaatata catcgctgta tcaagcatgt gaatgccata atcgatcaaa ggcccgcctc
     8281 cttgaagttc tttgtcgata aacgttcccc agcctggaac accagaccga cgaagcgcat
     8341 tcgcttctac gtaataaata tccccgagaa catcaagatg attcttgatc attgttgctt
     8401 cgtcagaaaa acgatgctgg aatccataag ccaaaatctg cccgttttta tcggctgtct
     8461 ctgccatctt tcttgcttga tcagctgtca tagctggtgg tttctcgcac atcactgaag
     8521 cattagcttc gagggcagcc aatacatgtt cgaagtgaaa gcgattgggt gtacagacac
     8581 tcacgatatc gggagaaatc tcattcagca tttgtgtaac tgatgtatac gcatgagaaa
     8641 atcctgtacg tcggcaaaag tcttctgctc gctggctgac tggatcaacc acagcggcca
     8701 attctaagac atctgatctt ttttgataat aagcagcatg tactttttct gctacttgtc
     8761 cagcgcctat gattgctact ttcttcattt atttctctct cctttgctta tttacttatt
     8821 gttattcttt ctctattctt tctctaaatc atttaaatga cgcgctgttt ccagacagtt
     8881 tttcaagtat tgcaatgatt cctgataaat tttcacttca ttttctcctc gcaaacgaca
     8941 ttcatatacc atataacctt catactcatt ttgaagtagc gtttgaaaat ggcgagtaaa
     9001 atcaattgcg ccagatcctg gttggtagcg atgattatct gctaaatgga tatgacccac
     9061 atactctttt tgcgtagtca atgcttttga aatatcatct tcttcaatat tcatatgata
     9121 aaaatcagcg atgatctgca catgtttcaa ttgattagag tcaatgtaat gacgcgcatc
     9181 cgctaacaaa ttgatcatat ggtcttggta tcggttcaaa ggctccaaat aaataaccgt
     9241 acctgtttct tcggctactt tatctaaata acgaagtgat tcgctgactg ctttatagtc
     9301 tccttctttg cttcggggtg aaaccatcgg cggtaatcga taagtgaaca ttccccaagc
     9361 tgctgggata acgattcctt tgcctccaac ctcgcttagt gcacgcaaaa tctcagcaat
     9421 ttgacgtaca ccattaagac gccgttcttc gataaagtca ccgatccacc catcgtagcc
     9481 gttacaagct gtcactactg ggatccctgt ttctttgatt gcctgtttga ctgtgtcgat
     9541 attttctatt aacaacttac cgtcgatttc gtattcttca aatccacagt ctttgataaa
     9601 tctaaatttt tctaaaatgt ctcttgggaa aaatgcttga tcttgcgtac cgaatttcat
     9661 tgttctcctc ctcggacttg aacgcccatt ttgatacttt cttctgggtg ctggtctaca
     9721 taacgcatgt aagcttctgc gctttctgca aatggcacaa ctggatcaat gatttcttca
     9781 caatccaagt aaccgttcat caataattcc caacatgtgt cctcgattcg tttacggttc
     9841 catcttgggt aatctgggtt tggttcacta gatgctcgag caaagacgat ttttgcatta
     9901 ttgaaatgtg cttcgcgacc aagattaaac ccagctggaa aaggtttagc aaaggcaaca
     9961 taagaaatga tcccgccata gccgatccct attaaagaag cttgtaatgc ggcagcactg
    10021 ccactcgttt caataactga atctactcca cgtttatctg tcaattcctt tagacgaaaa
    10081 ccgacatctg ctttcatagg atctagcgtg taatcggcac cgtgtttttc tgctaattct
    10141 ctacgctgcg ctatgggatc gatcccaacg atggtagtag cacctgcttt tttagctaat
    10201 tgaatcgcaa tttgcccgat agcgcctaaa ccgaagattg ctacagcatc acctgctcga
    10261 acatgtgctt cccggatacc acctaaagcg aattgagcag gatcgtaaca tactgcattt
    10321 ttccattgat ccaaccgatc aagtttacgt agtttataat tatcaacacc gtttgtaatc
    10381 agcgtttcac gaatgggtcc ataagtgcag actaactctc ctttttgata ctctgtcacc
    10441 aaactgcccg tttcttcgat cgtaccgaca atcatatttc ctaattgaaa ttcgccaaat
    10501 accacaccgc gttggcttcc ttcttctctt ggcaagaata ggcgccattc ttctgaaaat
    10561 tcttcatcga taaatggact taatcctcta aagtcgacaa tttctgttcc tggttttgga
    10621 gtagcgaaaa ttactcggat acgtacttcg ttcttttcga tctcacggtc ttcgtaatct
    10681 ttgaatccgg ctattcttgg ttctaatgca actaattttt tcatcttttc tttcctcttt
    10741 cttttctttt tatcctttta cgccgccttc gactaaatca cctttaataa atcgttcaga
    10801 taacgcatac ataatgatga ctggtaaagc ggtcaccaat gaagcggcca tcattcgtcc
    10861 ccaaatgtag tctggtgtac tgaataatgt gttcaaaccg attggtaaag taaatttttc
    10921 ggaggtcgac aggaaaatcg aagcaaataa ataatcattc cacgaaacca taaagcaata
    10981 aacgaagaca gaaatgattc cagaaatagc caatggtata atgatccgaa taatgatctg
    11041 gatccggcta agaccatcga tcattgctgc ttcttcaata tcagtcggaa tcgtgtcaaa
    11101 ataacttttt agcatgaata tcgcagtagg aagtgtttga acgaccatcg tgataatcag
    11161 tgctgtattg gtatcataca aacctagacc ggaaatgatt ttaaataatg gaacaacgat
    11221 caaaataccg gaaaacatat aaacggtgta aaagctagca ttgattttcg agcgagcttt
    11281 gaagcgtagt tttgacaaag cgtatgcccc gctgatccca atcactactg cagttacgga
    11341 ggctatcaag gagatcacta aactattttt gaaatacatg atgaatggaa aaatctcagc
    11401 attgaaaata tcctgataat gttcaaaagt ccattctttc ggaaaaatag tttgtgtcgt
    11461 tgcaattgct tcttgtgaat ccttgaatga tgtcatcagc atgatcaaaa atggaaacaa
    11521 attgacagct aatagcaaca aaatcgtcgc gtaaaataag attttcctgc tctttttatt
    11581 tttcatttca agtccacctt ctttcgtgag agcaagacga ccgctgcaat aatgatgaac
    11641 aatagaatcg atatagctgc tgcttttcca agatcattga acgcaaaggc tgtctgatac
    11701 aaataaacgc ctaagatatt gactttattc gtcagtagat agacatctgt aaacatatag
    11761 aacatccaga tagatcgtaa aacaatcaca gttgctagta ctggtttgat tgccggcagt
    11821 gtcacgatct tgaacttctg ccaagcattt gcaccgtcca tttcagctgc ttcataaagc
    11881 gttttgtcga ctgtttgcaa aatagccaaa aatgaaataa acgcatacgg atagtatctc
    11941 cagatagcaa acaaaacgac cataaagaag ctagcaactg gctggtcgaa ccacagcggt
    12001 gcttcgttga aaagctgcaa cctatctact aacagatcat tgacgattcc ataactatta
    12061 ttgaacatat atttccaagc aaaaatcaaa gaaatagatg gcgtaacata acttaataaa
    12121 atcagtgaac gcaccgtttt cctaaatctg aacttgcggt taagcaaaat agctacggct
    12181 aatcctaaag ctgtactgcc tgctacaact aagaatgtgt aagcaactgt taaacctaaa
    12241 gaacgataaa attcactatc acttaaaatt tcaagataat tgcttaatcc gataaaaacg
    12301 gcgtctgagt taggattcag cggcatatcg aaaaaactga tctgaatatt ggaaagcatt
    12361 ggaatcagca ctaaaacagc taataaaagc atgcttggtg ctaaaagtcc catggctaat
    12421 tttaagtcag aatgtttcct tggacgattc attgaaacct ccccctgata atctgccaat
    12481 agctatctac ttttttcgaa caccattggc agaacttctt gttatttcgt atttttttgt
    12541 gcgtcagcta aagcttgatc gacatcttcg tttccgacag ttacttggtt cactgctttt
    12601 ccgatcacac ctgagcttgt gatatctccc attttcgtga aattcttttc cccgactagt
    12661 ccaaagactt gtacttcatc aaaagcacta gcaatctcag atggcagctc gccaaaagac
    12721 ttgatgactt cattttcttt gtaaccatcc agttcgacta cttgtttatt cactggttga
    12781 gcaccaccag gagacatcaa gacccacttt tccatgtttt tcggttgaga taagaaagct
    12841 gtgaatgctt cagcagcctc tttttgtttc tcgtctagac cagaagaaat cgtcaatcca
    12901 gatactgtgc catagacagc ttgatctttt tgtgttggga tagcaaaacc gatattttct
    12961 gagttccccg cttcataaac agaaggcaaa atgtaagttg aatagattgc cattggtgct
    13021 gttccattca tgaaagcatc tttgatttct gtcacatcat tagagcctgg catcgtgtat
    13081 tgggataagt tttgataaaa cgacaatgct tctctcatct tttccgtatc gatcgttacc
    13141 tcgccttcat catccaaaac atttgctcca tttgacaaag caaattgaga gaacgcttgc
    13201 tcactcattg ttccttcagc agtaggcata gcaattccat attgtttatt ctctttatct
    13261 gtgaaatgtt gagcaatttt taaaatatca tcccaatttt ctggttctga aaaaccagct
    13321 tctgataatt tttctttgtc ataccaaata ccttgaaccc agccgctaat tggtgcagca
    13381 atatagcttt ttccatcttc tgaacgaaca aggtttttgg ctccgtcata ataattatct
    13441 tctccagctt cctcgataac gttttggact gccgtctgat caatcagttc atctttatcc
    13501 atcactttag caaaatcctg actgacttcc attacttctg gtaatttacc ggaacgagct
    13561 aacgtaacga tttttgtatt gaacgctgat tcttctaccg gcacttgctt gattttgatg
    13621 tctggatttt ctttttcaaa atcagcaacc agttcattga tcacacttaa tcgttcctgt
    13681 tcgacagaag agtgcatgaa ttgaatcgtt gtctgttgtg attcgttaga gctttgactt
    13741 tcaccacaag cagtaagaag aaatccagta gctagcagac aggtagtaac aataattttt
    13801 ttcatttttt tccacctctt attttattat tttcagccac atatattcat acggctggaa
    13861 tatcaacttt cgttcttgac tgcaatggat aatttgtcca gaaaacaggt ctaggtaatc
    13921 tccttttggt aaaagataac ctgctgattg attggaaaaa ttatggataa ctaagacttt
    13981 ctcttccttc gcttcgcgaa taaaactgat aaaagaatct gctgagtcca gtaagtaaat
    14041 caaactattc ggatggaagg ctttttcttt ttttcgaata gatagcatct gggtcaattc
    14101 accatatact gctgttctta atggatctgt ctgcaactct ttctcaatat ctgctaacaa
    14161 aaatttttgc cgattgattt ttcgattctc tcctgttttt tctacgcctt tgtaatcatt
    14221 tcgacttccc agcacacttt gaatataaat tgccggcacg cctggtaacg ttaccaaaaa
    14281 tgaatgagca gctaaaaagc gtttgcttct ccattccgtc gtatctgttg gagaacttaa
    14341 cgcatctaga tacgtagcgt ttatctcata tggactggtc gttccatctg ggtttttctt
    14401 gtagtttact cgggcaccct ctttcttcag ctgttccact aattcaagga tcgttttttc
    14461 cggcagaata cctcgtaccg gattcaatcc gataccgtca tgggaggcta aaaaattaaa
    14521 aaaagttgtt tttttcgatg cgaaatcgat ggtttttaac cattctttca acttacttgt
    14581 attacccgaa cgaatcgctt gaagggtcaa aggtggtagc gaaaactgat agaccatctg
    14641 tgcttcattc tcgccattcc caaaataact gatattatct ttgtgtggca catttgtctc
    14701 agtgatcaat accgttcctg gagctgcttc atcagcgatt gcacggaata attggatgat
    14761 tttatgcgtt tctggcaggt gtatacaaga agttcctagt tctttccata aaaatcccac
    14821 agcgtccaag cgaataaatg aagcgccttt ttctaagtaa aacaaaagaa tatcgatcat
    14881 tttcaataaa acttctggat ttttaaaatt caagtctact tgatcttcgc taaaagtcgt
    14941 ccatacgtgt ttttctttcc cgtttttgtc aatgaacgtt gtaagtaacg ggctggtcct
    15001 tggtctagtc acgctgcttg tatctgtatc cagtggcaaa tcaataaaaa aatctttgta
    15061 cgtatcattc aagtttaaat attcctgaaa ccaagtactt tcggcagaca tatgattgca
    15121 gacaaagtcg aacattaacc gtgtttcgct tctcatttct tctatatctt cccatgtgcc
    15181 caaacggtcg ttgattgcct gataatttac aacggagaaa ccatcatcag atgtatatgg
    15241 gaaaaatggc aagaaatgta caactctaaa tgtatctgcc aaatactttt gataaaattt
    15301 cttaaatgta cgcaaagttg gttgctgctc ttcaaaaaat tgatccccat aagtaatcaa
    15361 gaaaatatct tcttcgtccc aatgcagttt gcctgcctgg cctttcttct gctgataaac
    15421 actgactttt tgttcaatca tagaggcggt tttctcagac aaatctgagt cgcgataaat
    15481 cgcttgtaaa agttttgtat acatcggatt cccctttctt ttttgggagc gttcccaacg
    15541 atttgatcat attatggaaa acgcttactg tcaatagcat ttttttaaat aattttgata
    15601 tacacaaaaa tacaaataag aaagcttatg tatcaataat taatagcttc ttaaaaaaat
    15661 atggtctctc atgatcatgc aagctattcc taaattttca aaatcataga caaaaaagaa
    15721 tgtctattgt actatggaat tattctaaaa cgctcccaaa aaggaggtgc atataaaatt
    15781 gaaacctact atttatgaca ttgcgaaaaa agctaatgtc tcaaaatcaa ctgtctccag
    15841 agtattaaat aaccagccaa atatctctga taaagcaaaa gcaagtgtac ttcaagcaat
    15901 caaagaattg aattatgaac cgagcaaaat tgcaagagga ctgtcaggaa atggttttga
    15961 cgcgattcta gcattatctc atcgtacttc tcatacgacc tatggcaatc cttttttttc
    16021 agatgttatt caatccattt ctgccgtttc tgaacaaaat aacttcgact tgattctaca
    16081 aacggcaaaa acaccagaag aagagctaga aaaatgcgcg aaaaagatcc aagaaaaaat
    16141 gatccgagga attattattt taagctctac gatggatgaa acacttcttt ttgaattgga
    16201 tcaatataat attcctattg tggtaatcgg aaaagtcgga aacgactaca cgaacatcta
    16261 ctcagtggac acggataatt tccaggatag ttacgatttg gtggaaacac tgatcaagaa
    16321 tggacaccgt catatcggct gtctccatgc tccgacagaa gtttatgtat ccaatgatcg
    16381 tctcaatggt taccgtcaat gcttatttga tcaccaccta gatatccgta atgaatggat
    16441 tatagacggc ggctttcgct tagaagatag catggcagca atgaatgatt taatgaagct
    16501 acgagaaaga ccaactgcga ttttttctac cgatgcatta aaatcattga gtatttacaa
    16561 agtggcagaa caatctggaa agcagatacc tgatgatctt tcgatcgcag gattcagtga
    16621 tcccttattg tcctccctat tttcgccaca gttgactcgg atagacattc cgacaaatca
    16681 gttaggaatc aaagccacag aacttttatt ccaactaatc caccaccaat accctgaaga
    16741 aaaaagaatc attctttcaa ccgagattac tctcacagat tcgatccaac aacaagaaat
    16801 gtagaacaga attataaaag aacagaaaca ctgaggataa gtcagttact ctgttctttt
    16861 ttcaatcact catccctcgt taccccctcc cggtatttta gcgaattttt cttattttcc
    16921 atctgtttta gatagctgtg gataactata atgtttgtgt taaactattt tttccacagg
    16981 gaagaaaaaa ctatttttaa ccacgttgtg tataagtttt tatagttcgt ttttttaaac
    17041 acatttaccc ttatttcaat ccatgttatt tggggatact tatctctaaa acataaatag
    17101 aataacagac atgtctactg gttttgtttt attcgaggaa ttattcaata atagctagca
    17161 aaatattaaa aataaccgct ctatcttccc atcaatttat tttgcattca aaaaatgatc
    17221 tatctttact tttgttgatt gacttctctt ggaagatgat cgttccgata ccttaattgg
    17281 atacgaaccg atgttgttaa gatcctttcc cacttttctt ctagttagac aaattatttt
    17341 agacaaacca aaaaagaggt tgtgacaata atttgtcaca acctcttttt taaaatatct
    17401 attttgtttg attgttcatt gcgtcttctg cagccatttg cgcttcaata tctgaaatac
    17461 tgtagacatt ttcgcttgct acaccgactt cttttggttc catgtcacga taacgttgca
    17521 taccagtacc agctgggatg atcttaccta taataacatt ttctttcaaa ccaagaagag
    17581 gatctttctt accgcggatc gcagcatccg tcaagacacg agttgtttct tggaatgatg
    17641 cagcagataa gaaactattt gtttctaatg atgcttttgt gatacctagt aagacaggtc
    17701 gagaagttgc aggaacgcct cctgagacta gcgtattgta gttttgatct ttaaagtctg
    17761 cgatgtctaa taatgtacct ggtaagatat ctgtatcacc tggatccatt acacgaactt
    17821 tacgcagcat ttgacgaacc attacttcga tatgcttgtc gccgatttct accccttgca
    17881 tacggtaaac acgttgtact tcacgaagca agtagttttc aacagataat acatcgcgaa
    17941 cttgcaacaa ttgttttgga tcgattgatc cttctgtcaa tggtgcacca cggtggatca
    18001 tatcgccttc tgctactttc atacgtgctg tataaggaac agtatacgta cgtgtatctg
    18061 tttttccttt gattgttact tctttggtac gtgttgcagg atcttctgag atatcgatga
    18121 catcccctgt tacttctgtg atcactgctt gccctttcgg attacgtgct tcaaagattt
    18181 cttggacacg aggaagacct tgagtgatat cgtctccggc aaccccacct gtatggaatg
    18241 tacgcatcgt caactgagta ccaggttcac cgattgattg agcggcgatc gtaccaactg
    18301 cttctccgac ttcaacgtca gagccagttg caaggttgcg tccgtaacaa tgtttgcaga
    18361 caccatgttt tgtattacat gtgaagacag aacggattgt cactgtttcg acacctgcat
    18421 caacgatttg ttttgcgatg tcttcggaaa tcaattggtt tcctgcaatg atcaattcat
    18481 ttgtttcagg atgacgtact tctttacggg tgtaacgtcc cactaaacga tcttctaatg
    18541 attcgatgat ttcatttccc tcgcggatgg cttggatttc aagacctcgg tcagtaccgc
    18601 aatcttcttc acggatgatt acatcttggg ccacgtcaac cagacgacga gtcaagtaac
    18661 ctgaatcggc agtcttcaag gctgtatcgg tcatcccttt acgagccccg tgagttgaga
    18721 taaacatttc caagacggat agtccttcac ggaagttcga aatgattggc aattccatga
    18781 ttcgaccatt aggagcggcc atcaaaccac gcattccggc aagctgtgta aagttggaaa
    18841 tgttaccacg agctccagaa tcagacatca tgaagattgg atttttcgct tccaaacctt
    18901 ccatcagttt ttgttggatg ctgtcttttg cagcattcca aacagcaatg acacgttcat
    18961 aacgttcgtc atctgtgatc agaccacgac ggaattgttt tgtaatagtt tcaacttgtt
    19021 tatgggcatt ttcaatgatt tcttgttttt cattcagtac actgatgtca gcgatcccta
    19081 ctgtgatacc ggcatgtgtc gaatgtttgt accctaagtc tttcatacgg tcaagcatct
    19141 tagaagtttc tgttactttg aatcgtttga agacttctgc gatgatgttt cctaagtttt
    19201 tcttcttgaa cggtaatact aattcttgct ctttgatatg tgcaggaata tcagtaccag
    19261 cttcaacaaa gtatttgtcc ggtgtttgga cggtcaagtt gaagtctgtt ggttcattca
    19321 gataagggaa ttcaggaggc atgatctcgt tgaagatgat cttaccaact gttgtgatca
    19381 tcgtacgttc tttttgccat tcagtaaatg gtttttcacc taatgagtta gggttcacac
    19441 cgatacgtga atgtaaatga acatatccgt tgcgccatgc gataacggct tcgttttgat
    19501 ctctgaagac cattccttcg ccttcgcgtc cttcttcttc cattgtcaag tagtagttac
    19561 ctagaaccat atcttgagaa ggagtaacaa ctggtttacc atctttaggg ttcaagatgt
    19621 tttgagcagc taacatcaac atacgtgctt ctgcttgtgc ttcttcattc aatggaacgt
    19681 ggacagccat ttggtctcca tcgaaatcgg cattataagc ttcacataca agtggatgca
    19741 aacggattgc gcgtccttgt actaaaactg gttcgaatgc ttggatacca agtcggtgaa
    19801 gcgtaggtgc ccggttcagc aatactgggt gttccttgat tacgtcttct aaaacatccc
    19861 atacttcgtc ttcttgacgt tcgattttac gtttcgcatt tttgatgttt gaagctagtt
    19921 cgcgttgaac aagctcacgc atcacgaatg gtttgaataa ttcaatggcc atttcttttg
    19981 gaagtccaca ttggtacatc ttcaagaaag gtccaacaac gataacggaa cgacctgagt
    20041 agtcgacacg tttacctagc aagttttgac ggaaacgacc ttgtttccct ttcaacatat
    20101 gagaaagaga tttcaatggg cggttacctg gtccagtaac tggacgtccg cgacgaccgt
    20161 tatcgatcaa tgcatctact gcttcttgca gcatacgttt ttcattttga acgatgattc
    20221 caggtgcatt caagtctaac aaacgtttca aacggttatt acggttgatt acacgacggt
    20281 aaagatcatt caagtcactt gttgcaaaac gtccaccttc taattgaacc attgggcgta
    20341 aatctggtgg aataacaggg ataacgtcca taaccatcca gcttggttga tttcctgaag
    20401 cgcggaatgc ttctaaaata tccaaacggc ggattgcacg tgtacgtttt tgtccttgtg
    20461 cagatttcaa ttcttctttc aattcggctg cttctgcatc tagatcaaca ttatcaagta
    20521 attgtttaat cgcttctgca cccatagcag cttggaattc ttgtccatat tgttcacgtt
    20581 tttcgcgata ttcgcgttct gtcaacaatt gttttttctc taaattcgta tttcctggtt
    20641 cgatgactac atatgaagca aaataaatga tttcttccaa agcgcgtggg ctcatgtcta
    20701 atacaagacc catacgtgaa ggaatccctt taaagtacca gatatgtgaa acaggagctg
    20761 ctaattcaat atgtcccatg cgttcacggc gaactttcga acgagtaact tccactccgc
    20821 atcgatcaca aacgattcct ttataacgga tacgtttgta ttttccgcag gcacattccc
    20881 agtcttttgt aggaccaaaa atccgctcac agaacaaccc ttcacgttca ggttttaagg
    20941 tacgatagtt aatcgtctca ggttttttta cttcaccgta agaccagctt ctgatttttt
    21001 cgggagaagc caaacctatt tgcatacttt cgaatttatt tacatcgatc aaaaggggtt
    21061 ccctccattt catttaatgg caaaccgcaa gacggctgat cttcgaccgc cttgcagctc
    21121 atagaccgga ttagtctaaa ttatcttctg cggtttcaaa gttttggatg acatcgtctt
    21181 tttcatcttc cacttgttca gcagctttct tttctaattc tttggcagtt tgctgttcgg
    21241 caaattttgt caaagcatca acagtgatca aatcgtcgtc ttcatcgtcc atgtcgcgaa
    21301 gttcgatctc tgtttcttcg atatccaaga cacgcatgtc cagacctagt gattgcaatt
    21361 ctttcaccaa tacgcggaat gattccggta cacctggttt tggaattggt tcacccttga
    21421 cgattgcttc gtatgttttc acacgtccga ctacgtcatc tgatttgtat gtcaagattt
    21481 cttgtaaagt ataagccgca ccgtaagctt ccagtgccca tacttccatt tcaccaaaac
    21541 gttgtccacc aaattgagct ttacctccca atggttgttg tgtaaccaat gagtaaggtc
    21601 cgattgaacg tgcatgcaat ttgtcatcga ccatgtgagc aagtttgatc atgtacatca
    21661 caccgacaga cacacgtcca tcaaatggtt cacctgtacg tccgtcgtac agaacagttt
    21721 tcgcatcgct ggccataccg gcttcacgaa ctgtttccca tacgtcttca tcacttgcac
    21781 catcaaatac tggcgttgct acgtggatac ctaactgacg tgcagccata cccaagtgta
    21841 attccaatac ttgcccgatg ttcatacgtg aaggcacccc taaaggattc agcatgatat
    21901 cgattggtgt tccatcaggt aggaatggca tatcttcttc cggcataata cgggatacaa
    21961 cccctttatt tccgtgacga ccggccattt tatccccttc atggatttta cgtttttgaa
    22021 cgatataaac acgaactaac atgtttacgc ctggtgacaa ttcatctcca gcttcacgag
    22081 taaagatttt cacatcgtga acgataccgc cgccgccatg tggtacacgt aatgatgtat
    22141 cgcggacttc acgagctttt tcgccaaaga tcgcatgcaa tagacgttct tctgcagata
    22201 attctgttac cccttttggc gtgactttcc ctacaagcaa atcgccatct tggacttctg
    22261 caccaatacg gatgataccc atttcgtcta aatcttttaa tgcgtcttcc ccgacattag
    22321 ggatttcgcg tgtgatttct tcaggtccta attttgtatc gcgggcttct gattcatatt
    22381 cttcaatatg gatagaagtg tatacgtcat cttttaccaa acgacggctc atgatgatcg
    22441 catcttcata gttgtaccct tcccaagtca tgaaaccaac taaaacgttt tggcctagag
    22501 ccatttcgcc ttgttccata gatggtccgt ctgccagagt atcccctttt tcgactttct
    22561 cacccaaatg aacgattgga cgttggttgt agcttgttcc ggagtttgaa cgacggaatt
    22621 ttgttacatc gtatttatct aatgcaccgt tgtcgcggcg tacgcggatt tcagaagcat
    22681 caacgtattc aacgacaccg tcatgtttac ataacaaagc agctcctgag tcatgcgctg
    22741 atttgtattc cataccagta ccaacccatg gtgcttgagg attgatcaac ggcactgctt
    22801 gacgctgcat gttggcaccc atcaaggcac ggttggagtc atcgttttcc aagaaaggaa
    22861 tacatgctgt cgcaacggca actacttgtt taggtgatac gtccatgtag tctactttat
    22921 caatagatac ttctaagttt tcactttgag cacgagccat aacaacgtct tgagcaaatg
    22981 ttccatcttc attcaatggg ctgtttgctt gggctacgat gtaatggtct tcgatatcag
    23041 ctgtcaagta gtcgatttgg tcagtaacac gacctgtttc acggtctaca cgacgataag
    23101 gtgtttcgat aaatccgaat ttatttactt tcgcataact ggacaaacta ttgatcaacc
    23161 cgatgtttgg gccttcagga gtttcgatcg gacacatacg gccatagtgg gagtagtgaa
    23221 cgtcacgaac ttcataaccg gcgcggtcac gagtcaatcc gccaggtcct aaggctgaaa
    23281 gacgacgttt gtgtgtcaat tcgccaagag ggtttgtttg atccatgaac tgtgacaact
    23341 gagaagaacc aaagaattct ttgatacttg ccacaactgg acggatattg atcaattgtt
    23401 gaggtgtcaa tgtttcagta tcttggatcg acattctttc acgaaccaca cgttccatac
    23461 gtgctagccc gatacggaat tggttttgta aaagttcacc gactgaacgg atacgacgat
    23521 ttcctaagtg gtcgatatca tcgacattgc cgataccttc catcaagttc aagaaatagc
    23581 tcattgaagc aatgatgtcg gctggacgaa ccgtttttac agctgcttct ggatagccgt
    23641 ttccgatcac gtttacttca cgttcagggt ctcttggtga aaatactttg atgacttgga
    23701 cagtcattgg atctgtgacc acaccgtctt ctgatggata gtaagtcact gaatttaggc
    23761 cattatcaat gaaaggagct agtgtttcca tgacttggtg agtcagaact gttccttttt
    23821 caacgatgat ctcgcctgtt tctggatcaa ctaatgtttc agctaatgtc agattcaata
    23881 gacgtgtttt caaatctaat tttttgttta ctttgtaacg accaacgttt gctaagtcgt
    23941 aacgttttgg atcgaagaaa cgagcattca gcaagttacg tgagctgtct gctgtttttg
    24001 gttcgcctgg acgcaaacgt tcgtaaacgt ctttcaaacc ttcttctgtt cttgagtcgc
    24061 ttgcgttttt atgcaagtct ttttcgactg tattgcgtaa tgtttcgctg tcgccgaaga
    24121 tttcaaagat cgtatcatcg gaaccaaatc ctaacgcacg aactaatacg gttaaaggaa
    24181 tcttacgtgt acggtcgata cgtacataag agatgtcttt tgcatcagtt tccatttcta
    24241 gccaagctcc acggttaggg ataacagtag aaccgaatcc ttcttttccg tttttgtcga
    24301 cttttccatg gaaatagaca cctggtgaac ggactaactg agaaacgatt actcgctctg
    24361 ccccgttgat gatgaatgtt ccttgttctg tcattaatgg gaaatcaccg aagaatactt
    24421 cttgtgcttt gatttcgcca gtctcgcggt ttgtcaaacg caatgttaca tgtaatggtg
    24481 cagagtagtt tgcatcatgc gcacgagctt ctgctactgt atactttggt tctttcaatt
    24541 cataatctac aaattccaaa gatagattgc cattgaagtc atcgattggc aaaatgtctt
    24601 caaacatttc tctaagtccc tcatctaaga accattggta agagtctgtt tgaatttcga
    24661 tcaaatttgg taattccaac acttcactga ttcttgcgaa acttctacgt tcgcgatgtt
    24721 ttccgtattt tactacgtgt ccagccaagc tcttcacccc tctaaaatgt cattcaaaaa
    24781 gactacccga tcaattgtta caataatatt tcaaatatta tttttaggta acaacagaca
    24841 ttttttgcgg cttttaggca aaaaaaaacc aaaaatagaa cattctcttg aaaccttact
    24901 tcgtgagctt ctacttttgt ttccttatat taaagccgaa acggacaata tttcgtttct
    24961 gccttttctt catcagatag tttttgcaac ttcatatact acacttattt tctataaaag
    25021 tcaacacttc ctggcgtttc ttctgctttt tcgcaacatt ttcttaatgt tttcaatttt
    25081 atgttttcaa ttatctgaca attcttaatt gacagacctt tttatctcgg ttatacttta
    25141 gaaaattcat tttgtggagg aacttctatg cgaaccaaca tcaaccaaat cgcaccaaca
    25201 caactgaata acttttactt tagctacttt agataggttt gtattcatca tgatggatac
    25261 aaacgttaaa cgacagtttg tatctatgat ggctaaagaa ctttgtattg cagcaaaaag
    25321 ccacgtagac aaaatgctaa gtggctgagc agtgaaaata caaagtaaaa gtatcaagca
    25381 ttctactttt tcaatttatt ttccgacttt tcaatccatt tggacatttt gtctgaatgg
    25441 attttttgtt tcccaaaatt aaggaggaaa aagaaatgaa aaaaaatata atcatcggat
    25501 taggcgctgc cctgctacta gctttaggag gttgcgcaca aaaagacaat tccgcagagg
    25561 catcagatgg acaaaaaaca attggcgtat tgcaacttgt acaacataac tcgttagatg
    25621 ctgcctataa aggattcaaa gaaggattag cagaagaagg gtataaagaa ggcgataatc
    25681 taacaatcga ttatcaaaat gcccaaaaca atcaagacaa cctgaaaagt atgagtgaga
    25741 aactcgtcaa agaagatcct gatttgctac ttggaatcgc cactccagca gcacaatctt
    25801 tattaaatga aacaaccact atcccgatga ctgtcacagc ggtcacagac ttggaacaag
    25861 cccatttagt cacatccaat gaaaaacctg gcggcaatgt gactggcacg acagatatgg
    25921 tgccaatcga aaaacaaatc ggcttactgt taaatatcgt gccagacgcc aaaacaatcg
    25981 gcatcatgta taacaacggt gaagcaaact ctaagatcca agctgatttg gcagaaaaag
    26041 aattgaaaaa agcgggcgtc aaagtcaaag tactgacagc aaattccaca aatgatgtcc
    26101 aacaagtgac gaaaagtcta gctaaagatg tagatggaat ctatattcct actgataaca
    26161 cgttcgcaag tgcggcaagt gttgtcggtg aagtcgcaaa agaaacaaaa acacctgtcg
    26221 ttgctggcgc agttgatcaa gtcaaagacg gcggtctagc aacagtagga atcgactacg
    26281 aaagcttggg caaacaaaca gggatcatgg cagcaaaaat cttagacggc aagaatccaa
    26341 gtgaacttcc tgtcgaaaca gcgaatgatt tggaactgta tgtcaataaa gacatggcca
    26401 aagcattagg catcaatcca gacagcatca aagaagctga ataaaacaca ggaggattct
    26461 catgacaaat ttgctctctc ttttcttatc atctgcatcg caaggggtct tatgggccct
    26521 acttgcgatt ggtgtatacg tcactttccg tatcttggac attgccgact tgactgctga
    26581 aggaagcttt cctttaggtg cagggatcgc ggcagtaagt attacaaatg gctatcatcc
    26641 attgacagct tgtcttctag gctttttagg gggtgcggca gcagggcttg tttccggact
    26701 gcttcacaca aaattgaaaa ttcccgccct tcttgcaggg atcatcacca tgactggttt
    26761 atattctgtc acatcccgtg tgatgggtgc gccgaacatc tctttattgg gtcagccaac
    26821 gatctttcat tgggtcgagt cattcggcat aacaaaagac aacgctgtac tgatcgtcgg
    26881 tctgcttttt gctttgatcg ttgtatgttt acttgttttg ttcttacgta cagaaacagg
    26941 acttgctatt cgtgcaactg gcgataatct ggcaatgagc gaagcaaacg ggatcaacac
    27001 agataatatg aagatcatcg gttatatgat ttctaatgga tgcattgctc tttctggctc
    27061 cttacttgcg caaaacaacg gctttgctga tttaaattct ggtattggaa cgatcgtaat
    27121 tggacttgct tctattatca tcgcagaagt tttgttacgt aaccagccac tatttctccg
    27181 tctcttgact gtcgttttag gtgcggtcat ttatcgtttt attttagcgt tagtcttcga
    27241 actgaatgtt gaaccaagtg attcgaaact tgcttctgct ttcgtgctag tgatctgtct
    27301 ttctttcccg cagatccagc aaaaattcca tttacctcaa aaaaagaaag gcggtagtgt
    27361 caaatgaatg acgtcttaac attggaaaat ctccatcata cctttgaaaa aggaacgatc
    27421 aacgaaaatc atgtcctgaa aggcattaat ctttccttag atcccggcga cttcgtgacg
    27481 atcatcggtg gaaatggggc tggtaaatct actttgctga acagcatcgc cggtacattt
    27541 tctgtcgatc agggtcaaat tttattgaat ggaaaaaata tcacgaaaaa atctgttgtc
    27601 gaacgttcga aatccatcag tcgcgtattc caagatccta aactagggac agccgttcgt
    27661 ttaacggtag aagaaaatct tgctcttgct atgaaacgag gaaaaaaacg cggcttcttc
    27721 cgtggtgtca aaccacaaga ccgaacattt ttcaaagacc agctggttcg gctaaatttg
    27781 gggttggaaa atcgtttatc ctcagaaatc ggtttgctgt ctggaggaca acgtcaagct
    27841 attacgttat tgatggccac attacagcgt ccagaactga tcttattaga tgaacacact
    27901 gcagcgcttg accctgctac ttccaaaacg gtgatggaat tgacagaaaa gctgatcact
    27961 gagcagaatc tcactgcttt tatggtcact catgacatgg aagacgcgat ccgctatggg
    28021 agtcgactga tcatgctgca tcaaggaagg atcgtcgtag atatccagca agaggaaaaa
    28081 cagcagctca cagtgcccca attattagcg ttgttcaaac aaaacagcgg cacggcatta
    28141 aaagacgatc aagtactttt atcttcataa tcagaaagag gatgggacaa aaaatgaacg
    28201 cccaccctct tcttttttct ctttccttct ttcgtttttg ctttatgtat caaccgttgt
    28261 ttcttcgaaa actattttgt tctctccttc ttcctgatag aatggatggg aaaggagctt
    28321 gatgccatga caacgaaaga caaggtactt caattattga aaaagacacc agattttctt
    28381 tccggagaaa agttggcaca gcaactggaa gtttcccgga caagtatttg gaaagccatc
    28441 aaagaattgg aaaaggcagg ctatcgtttt gagcacgggc ctacagggta tcgttacctg
    28501 ccctctgacg tactagatgc aaaagaaatc cagcaagagc tacggtcgtt tattcctgat
    28561 ttgaaagtac aaacgatgtt gacttctgaa tctacgatga aagatgccaa actggcagca
    28621 gctcaaggag attctgtttc gaagttattt gttgctgata cacaagaaca agctcgtgga
    28681 cgttttggac ggcctttttt cgctgaacct ggacgaggaa tctacatgag tctcttgctc
    28741 catccagaca aacggtttga tgaattgcct caatatacga tcatcgcagc cagtgcctgt
    28801 gtgaaagcca ttgaatctgt aaccggtcgt tctgcttcga tcaaatgggt caatgatatc
    28861 tacatggatg gaaaaaaagt ctgtgggatt ttatctgaag cagtcagtga tatggaaagc
    28921 ggcaggatca gtcatatcat cattgggatc gggcttaatt tttctgttcc tcaaaatcat
    28981 tttccagctg atctccaaga aaaagccatc tctgtctttc ccgacggaca agctagtgtc
    29041 acaaggaatc agctaatcag caaaatctgg caagaattgt tcacttatct ttctcaccca
    29101 gaaaaagcgt tggacttcta taaagaaaaa tcattcgtat tgggaaaaac cgtgtcgttc
    29161 aatcaaaaag gaacgactta tgtcggtctt gcaaaagaaa tcggtgatca tggcgaactg
    29221 atcgtccaaa caaatgaaca gaagatgagt ttgtcttctg gcgaaatcag tctcacatct
    29281 atccgtacgc aataattatc agatttcaca aaaggacacc tgatctcata cagtgtcctt
    29341 ttttctgaat tctcttcaaa agactctcct tattctatca gctgacagtt tctctatcct
    29401 ctagaaaaag gtattctaaa aatatctata aagcatggta cactttcttt aaatgaatca
    29461 atgcaaaaaa caagagagtc atcagtttac acgatcaaaa gagatagcag gccgcttcct
    29521 ttttcaatct tatagataga ccatgggtaa cgataaaaac aaaaaagaac ttgcgactta
    29581 ttttgccgta agttcttttt tagctagtgc cttaatctct taatcccaac aaacgtctcg
    29641 catagatcac ctgaccagag tgcatagcag catcatcaat aatagaaacg agacgcattc
    29701 ctcgtgtgac tggtggtgtc cagttctcgt caattacttc atctagctcg gaaggttcca
    29761 gccttcttaa gtaagaaata gcaaaatcag cagctcctcg caagtatcct agcagatctt
    29821 ctatttgatt agcttggatc tgctttgctt gttccagact atgcttccaa tcttgttcgt
    29881 tatctgctat cgtcatcgga aaccggcttt cccagtcttg actatgccaa attggagaag
    29941 ttcctgctaa atcagcaatc tggaaatcta attctcgagc ggtatgccaa gccagccaag
    30001 tcattgattt gatctgcggg gctctatctt ccactggaaa acgattggcc tcctcaatag
    30061 agacgccttc aaacaaacgt tcaaaccgat cgtatgcccg atccacactc tcgcatagca
    30121 tatctaaata atccatttat tttcctcctt tttatttatc ctacttactt tttcctaatt
    30181 ctaggatacc tacttatatg ttactacaca cccttatttt ttacacttta acagtcacca
    30241 tcatcaatag tttggagatt gaaagaaaaa gatagtgtcg aactatcaat tgaacaagaa
    30301 gcaacaatat gaacgtttca ttaatattct ttaaataaac aatgattttt caaacgaaca
    30361 gtttaagact aggagaaaaa gagtataata ggaaaatgtt tgaaaggaaa ggaaggggaa
    30421 aaatgaaaaa aagggcaagt ttactagtcc tagttgcttt gttgttcaat atcttttcgt
    30481 atggtgtggc cgtttcagcg gaaacataca ttcctaaaga ccctgggaat atttataatg
    30541 atctaactgg taactatggc ttgttaggaa ttgctagtca attccacgta tttgctaaaa
    30601 ataagacaac tgtgaatgca catacagacg gaaatatagc aacacatgaa cttgatgcaa
    30661 aaaataattt tggtacagag cttcatgaag gtttattgaa ccaagaaata aattatatac
    30721 aaaaaactga ttctttcgct aattcttcag gcatacctac aggtagcaca agaatgaata
    30781 aatttgtcgt tggcagtaac gtggatgtag cacttcaaga tggtcatcct acagtcaatg
    30841 gatccaggct tgatcactta actccagatg atttttataa agatcgtgct gggcatgaat
    30901 atattgattt cgagcaggaa tttgctaaat tgaatgtagc agcaaatgat cttgcaacga
    30961 tcactccagc taaaacatat actgctgcag attttccaga catgaacaat cggacaatcg
    31021 atttaactga tttatcagat accgggtttt tattggtaaa tatcgatgca gaagtgttaa
    31081 cgatgaatac gccacttcaa atcattaatc caaatgatca agtagttgta tttaatgtca
    31141 tcaacagttc ttctgcattg aacgtacagt caccaatcaa atacaatgat cgttcaaatc
    31201 atgaaacaga agatttttcg gatgcgaata tctcttggaa ttttggaaat gagatgactg
    31261 atttgacaat cagtgctcct tttcaaggaa caatccttgc gcctaatgct actatccgca
    31321 ttactcaaaa ccaagacgga acgattatag gaacaaatgt gattttagat gcagctacca
    31381 atcgttggga tcctaacgaa atatttatca gcgacaatgg tacagatacg acagacacaa
    31441 gcgacagtac agatacatct accacaagtg atagctcaga ttcatcaact tcaagcgaca
    31501 gtacagatac tacctctacc acaagtgata gcacggatac atcagct
//
LOCUS       DACEEZ010000025        27773 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20313_2017
            SAMN12639966-rid9879223.denovo.025, whole genome shotgun sequence.
ACCESSION   DACEEZ010000025 DACEEZ010000000
VERSION     DACEEZ010000025.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639966
            Sequence Read Archive: SRR10023158
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 27773)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27773)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741683.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741683.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:44:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,016
            CDSs (total)                      :: 2,962
            Genes (coding)                    :: 2,889
            CDSs (with protein)               :: 2,889
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 36 of 73
            Pseudo Genes (incomplete)         :: 29 of 73
            Pseudo Genes (internal stop)      :: 18 of 73
            Pseudo Genes (multiple problems)  :: 8 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27773
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639966-rid9879223.denovo.025"
                     /isolate="E20313_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            1..1218
                     /locus_tag="IWO89_001192"
     CDS             1..1218
                     /locus_tag="IWO89_001192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HAQ0455962.1"
                     /translation="MSNDTQPEIRFPGFTDDWEQRKLGDFVVDYVEKTSVQNQFPMLT
                     SSQQKGIVLQEDYFANRQVTTENNIGYFVLPRGYFTFRSRSDNDVFVFNRNDIIDRGI
                     ISYFYPVFTLKSADSDFFLRRINNGIQRQLSIQAEGTGQHVLSLKKFKNIVAMFPSEE
                     EQQKIGTFFKQLDDTIALHQRKLDLLKETKKGFLQKMFPENGAKVPEIRFPGFTEDWE
                     ERKLGELGKTQSGIGFPDAEQGGSEGTPFFKVSDMNNIGNEYEMRNANHYVSNEQIER
                     KKWKPIKDVPAIIFAKVGAAIMLNRKRLVTSPFLIDNNTMAYLFNNTWDIYFGKILFE
                     TINLPRYSQVGALPSYNSSDIENISVKVPVKDEQQKIGTFFKQLDDTIALHQRKLDLL
                     KETKKSFLQKMFV"
     gene            1231..4380
                     /locus_tag="IWO89_001193"
     CDS             1231..4380
                     /locus_tag="IWO89_001193"
                     /EC_number="3.1.21.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HsdR family type I site-specific
                     deoxyribonuclease"
                     /protein_id="HAQ0455963.1"
                     /translation="MKISHREEAEVEEQLIRVLGEGHNQWTYRPDLKSEEDLWVNLRQ
                     KIISNNQAELNDFPLTDKEFETIKTELLLRTKTPFDAAKWLKGENGMARITIEREDSQ
                     LGSVSLILYSNQDIGGGISTYEVVHQIAKRGSNIEARDRRFDVTLLINGLPIVQIELK
                     QVTAKDGVYQAFNQIKKYAEEGMFRNNIFSTLQLFVVSNEQTTRYFANALPKDLHPKF
                     LFSWRTKDNEKVENLYEFCKQVLNIPDAHRLIADYTIVSEDQDNKTLMVLHPYQVHAI
                     QALFIAANKHQSGYVWHATGSGKTLTSFVSTKLLARKSGIDRTIMLVDRKDLDNQTTT
                     EFTKFASEFNTGISSGNAKANSLIVGTGSAKELSETLLADANANVVIITTRQKLDAAL
                     KYAKKQEEKKGTNRFQKLMGQHIVFVVDECHRALSAENMEEIKKMFPKSTWFGFTGTP
                     IFPENRKQAKGQLARTTHDQYGEVLHTYTIKNALEDGSVLGFQVEHENTVEPTSLENK
                     IYRKLKEVETYAEYSSEQINRMIDQMEPVKKESYLDPAVFEADEHIQKVIHKMFRPDN
                     AYTKFDFRNGRPTKSAILTTSSIDMAKKYYRAIKEMTKEPDWLTKEFPNQLIREGRTM
                     EDPDFPRIAITYSLEENSRDSSAQQEEMQKIIEEYNQYYDTSWSLADIERYNGDINNR
                     LARKRAEFKQFGKQIDLAIVVDRLLTGFDAPTIQTLFVDRNLEYAGLIQAFSRTNRTY
                     PDKTKGLIVTFRKPATMEKNVEDATKLYSKAKEESGLIYPSYQESKKRFKQAYGKLKE
                     VSSMESIDEHTPLEIRIEYVKAFQELNRAYESLVTYDDYNDDMVKSPTLNNQIQLLEE
                     QIGVYETVKGSLIEEEPEKEGEDFSTIEFYSENTTKLYDIDSTYIDQLLGTYAANSSD
                     AREEIEKALAKLNKTEGVKEVYRQILNAFDVGTLDSNEDIFVIKRRYFTQASDDLIHV
                     FSNEWFVSEKELHASNLQYLPGEDPIPNMKAIINSKDYEGYKAKHPEAKPFKYPQEMK
                     RAWRKMLDDELIPLENELR"
     gene            complement(6315..6980)
                     /locus_tag="IWO89_001194"
     CDS             complement(6315..6980)
                     /locus_tag="IWO89_001194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAQ0455964.1"
                     /translation="MNTGEKIRTYRRNCKLTQKELGEKIGVSDKTISSWENSRTMPDL
                     EMLSLLHHTLGLPIDFPSIESATESIEPTLPDEPLVNKKSYSSKQPFSLKKTLSFFLL
                     VFCLVYLFLINYQYKNETIDRFNPFLKPQIGYTTLPVGYVDNSIERWVCNDIFGHGTW
                     LSFHAGEVEKEKNHAVIKHKGKHVMNVRLLTWEAIPSDYQEILSQDYDDALKRSPQEP
                     DRH"
     gene            7106..9142
                     /locus_tag="IWO89_001195"
     CDS             7106..9142
                     /locus_tag="IWO89_001195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1430 domain-containing protein"
                     /protein_id="HAQ0455965.1"
                     /translation="MIIKKKFTVLLTIIVTGIMSGFALVSINKQVLYRTNEFFHQTDT
                     YTPIRLPQHFIGQEEYEQLIMVFENVSSETELSYMKKTVNESNEVDTSWNFKIQPKLE
                     MTLYYQGDVKSSHEKVPYSNTSFRVTSIEESIKIKDFEGEFFLMTREKEAYERFISLF
                     TDRFNQAFHTDYATNIFEDFENSYSYSVISNTDVYNYRVYLTIGLILLFLLLSSFFLL
                     MNKEISLLLNNDFSLHQVLWCLLGKKKIIITFIIAGIIGGFIFMYYKEQFLYLFQMYV
                     FLFSGIYGISLISLLLVEKIASLREAFNYIQYILFTFGLLFSLLLTVANVELATIVFS
                     SSQLAVSHEPPKEIAGEDYHVFYPVTIGKNHVDFIYSNRFASAADQELYETLNKNGSI
                     LVNVSDYLNEENEQFGRGIKINLNYLKKFPLIDVSGRLIQITEKETHPVILIPERYRH
                     TDLKNDLAQITEYYQEISEKEPKFLFIKNNQPIYTFIPSIPWIEHYPVVEILTLKNSM
                     YLERNILSGEIYPPLKIKIGSLSKERLFELIEESQLRDNLQLSFPYTQTEEIAVKVLS
                     RSFHYLIQIFLLTFFSFFLLSYVMLCIYFVYNCRKIAIFRSSGYSLFETYKDFFMMNL
                     IKWGTTSVIFLFLIEREPKYLFNIFFFSFIDFILSVVFILSTEKKSQLLLMNGG"
     gene            9146..9784
                     /locus_tag="IWO89_001196"
     CDS             9146..9784
                     /locus_tag="IWO89_001196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ0455966.1"
                     /translation="MEPLIVIKGAKKIIRNKTILHLDRFTADAGDLVTVVGESGAGKS
                     TLLNILGLNDQFSSGQFYLFDQETKQLTGREKRLIKRNTISFLFQDFGLIEYETIDFN
                     LDIGLKFSNLNVSDKRTAKYEALKKVNINKKLSTPIYTLSGGEKQRIACARVLLKNSK
                     IILADEPTGSLDKRNKDKVVEILSQQASQGTLVIVVTHDPYLTKIGNKKIML"
     gene            9812..10240
                     /locus_tag="IWO89_001197"
     CDS             9812..10240
                     /locus_tag="IWO89_001197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0455967.1"
                     /translation="MKKAVIICALGIGFVGGNAMSSEVEANSVHGEIYYKSLKPGEIH
                     FDAKGNIIKSNLPVDREISRTAINHSSGRWVYYSDTTCTRKTGHSNHYSRVYNRHGAK
                     AKVGNSIRSANATKGVWAYASATGNRTDTFSCWYNPTGWY"
     gene            10697..11608
                     /locus_tag="IWO89_001198"
     CDS             10697..11608
                     /locus_tag="IWO89_001198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HAQ0455968.1"
                     /translation="MKKRFLALAIVLGTGLLSGCTNAGEKTAVSYKGGTISEQEVMDS
                     LKKMQGADSAVQQLIVYQVFEDKYGDDVSTKEIDSQYDQTKKQLGDSFDSQLKSAGYT
                     EQTFKDSIKQSLAFQEGLKKHIKLTDEDLKTAWESFHPEVEAQIIQVASEDDAKDVKK
                     AADKGDDFSKLAKDKSTDTTTKEDGGKVKFDSTTTTVPAEVKEAAFKLKDGQVSDVIT
                     STNASTYTTEYYVVKMVKNQNKGNDMDKYKKELKEIATDTKLSDSTFQNKVIGEVLKD
                     ANVKIKDKDFENVLSTFTSDSSTASSK"
     gene            11825..12262
                     /locus_tag="IWO89_001199"
     CDS             11825..12262
                     /locus_tag="IWO89_001199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0455969.1"
                     /translation="MEWFCKFLLFLEVIHVYGFVFLTNKKAGSTMDHLYIKYIHQFSV
                     LVLGTLIIAGIVAFIIQGRDILTVHQEFFSYAFGIPLIAIRSDNKKWKKRYYILLLFL
                     CFYLPPKIFTLLGIQCLSFSFISYIIVLIFLSFIVWMFYLVKK"
     gene            12357..13241
                     /locus_tag="IWO89_001200"
     CDS             12357..13241
                     /locus_tag="IWO89_001200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family lipid kinase"
                     /protein_id="HAQ0455970.1"
                     /translation="MKKVLLVVNPSSGGEQAKEFEQLAIAKLESVFDEVVVLHTKKAG
                     DAKNFTREAATEGYHSVFVMGGDGTVNEGISGIAEQEHRPNFGFFPLGTVNDLARALG
                     IPLEPEEAINHFSIESVKPLDIGKINDDYFMNVVAIGSIPESINDVDAEKKTKFGKLA
                     YFMSGIKQLASTQSYSFHVEVDGKKEEIESSTLLIGLTNSVGGFETLLPNAKVDDGKL
                     HLVYLKDSSLIDTLKTLPDLLKGVDESTKNLAYLTCEQLVVSLADEEELTTNVDGDEG
                     DKLPVTIKVLPSHLQVYC"
     gene            13465..16632
                     /locus_tag="IWO89_001201"
     CDS             13465..16632
                     /locus_tag="IWO89_001201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0455971.1"
                     /translation="MNQCVKKLIASILFVFPLLMVTSVFADVEKTNESTQSENQSFDK
                     TTFSEESITSETTESQSEASTNKRQEENKTSETSEVAEQTSDSSEAALARAVSADGYS
                     EAATTEELAQLLADESVTKIRLIQPLTLDRELEIKRDIVIDFGGFAHNFGTHHIYINE
                     IPQSIEFQNFKGTAAHPGGLTPNIDGNAIIIAYMTDFWGSRYHFTGEIKFTGTLDLYD
                     GSKLGLIYAPRATVTLDGVSGVLDVQPVKEGLNAAPGKAYFCRSYQLNVINGSQLYGP
                     YLGKFYGFLDSGDETGSGKTAPGIHILSGSKVSLDYDRADGVSDEGEAVDTLPGNVMF
                     EVSGSGSEFSVNTKINITNDNNRGIIQMRGSGSKVTVSNGGKITINTATTGGFRLQGD
                     SSQIHVSSGGQISVKMAGDDDQPGNNGMRFVGKGLSLIVEGAGSVIDIDKQSGRSSAV
                     RFENGTQKLTVTNGGSLRVRNTGDGKSYVNRLNQGNQAIQFYDASGFLESPGSADFTV
                     TGEKSNIDIRADSGATVDTTGDIDLNFTAGEKTYLVMVGKTGEDKTGIISGDVLNVDL
                     ESPTYFDFQNKRIGSETNGIGGWVFQGNGDSSLKIAHSEIALWRKTGATFDNFEKDPD
                     YFSDMTDLSINGTDLGNFVSSPSAGLTNEFAQTGAGMKNYNRISANNQLPMIESLRVP
                     TNADKKIYGHVVVPEGVEAKPRDAWTGEVEVTLRITYADTTKSSVEITALTQGQTDEN
                     EGAGYNPWGEGEQGGLFEVQVPNDERLKTEDQVQVVAAKKVRGGQEVTELDSAKTTVD
                     VIPPEPANLSTTLINAASRTITGTGEAGALVSLKKNQAVLSETTIDDTGEFVLSIPEN
                     QLFAGDVLEIVLNDQAGEATAKGVQNKPSTNDEIGNHNPSDTPVVYHDAPPFAPATKI
                     VVEGGLSFLAPSKLDFGQIKATGLTQEAFGQMDIQEKLWIYDQRETKNPWILTIKLSQ
                     PFTNDAGFSMQDVLYFKKGEEYQLVNEEELEIVRGQNSTNVQADYSEYLNNGRAFKLV
                     LTKENQIAGNYSAEITWTLADAPTE"
     gene            16652..17197
                     /locus_tag="IWO89_001202"
     CDS             16652..17197
                     /locus_tag="IWO89_001202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014386985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF916 domain-containing protein"
                     /protein_id="HAQ0455972.1"
                     /translation="MNRIWNKSHRNNCSLLMILLLGFILWQTPVHASEFNFAVDFELP
                     ANQIDKNKTYINVRMEPGKTQEIPYTVFNRTDKSVTVETLIHSATTNSNGVIEYGESK
                     SKFDETLSYDMKNLAKTVGEITVPPQSAVTETLSLKAPEKELAGVVAGGITFQEKKEN
                     VSNKSTVGVVNQFSQVKAILI"
     gene            17225..17680
                     /locus_tag="IWO89_001203"
     CDS             17225..17680
                     /locus_tag="IWO89_001203"
                     /inference="COORDINATES: protein motif:HMM:NF023225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3324 domain-containing protein"
                     /protein_id="HAQ0455973.1"
                     /translation="MKLKKAYADQSDGRNVIKANLQNPNASYMFGLTTSVEIYKKETK
                     EPYLSIKKEKIDVAPNTNFDLLVPLEGKKLAPGHYQAVVKGAWKEKRWNLHTDFEITD
                     DAADQLNEKDIDLKNQPETFDWRWLILVGVILIGLFAGLFILKERRKKK"
     gene            17677..18435
                     /locus_tag="IWO89_001204"
     CDS             17677..18435
                     /locus_tag="IWO89_001204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WxL domain-containing protein"
                     /protein_id="HAQ0455974.1"
                     /translation="MKKTIALSTVLAGSLLLCTQSVFAENKSSATTEGTISVTKPDAA
                     NPEDVVPVPPVDPNDPDTPFTPDEPNQGTSGLLTIDQAPDLDFGTIKLGTAKTVYAAL
                     QSGTDSTGAQTQVQNFVQVTDKSGNYAGWKLSVKRTEFIGTTSDTNKLTGSKVKFKNA
                     VIRNSATNSSGMPSTVSGTGAEGIEIPADQAVDLVTAGANEGIGTWIYSLGENVEQGR
                     QSVELFVPVSNYAVDNYVSTFTWSLTDAPTEEEA"
     gene            complement(18521..19261)
                     /locus_tag="IWO89_001205"
     CDS             complement(18521..19261)
                     /locus_tag="IWO89_001205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAQ0455975.1"
                     /translation="MKKYFIIEDNQFHSEWIIRELVNHHIENIVQLTSISQINQLIQL
                     GTIDNHDVFFIDIDLKQSLTGIDVAEQIRKKNAHSNIVFFTAYTNFAVEIINRKILPI
                     GYIRKDRNLSEQIRQVLEQINWKEQQIVNVDKIFIDQKGGSLVLYPREILYIESIKGL
                     KNQVMVKTTTEDKLVNTSLKQLKTELEKYAYFLLLKSYCINLSQLQKIDSAHYVLEFF
                     NGDSLLVGRKIFEKTKERFHDFQKTASS"
     gene            19322..20575
                     /locus_tag="IWO89_001206"
     CDS             19322..20575
                     /locus_tag="IWO89_001206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HAQ0455976.1"
                     /translation="MVATVTLYLTFTLILDLGIYLFFSKQLLVKNSFGCIALMVVTLL
                     LPIPKVSFPYLVGLLLMFLFYFTTKNLFISCLISNLFFAITGLSFFLVFDLMDYLGKR
                     HLGTASFFALLIAFIFYLLVKSIDKQTRFLNLLFTYPTEHYFGGICILLSWGVIYLVL
                     GSVHFRSSNYLLLSLLSIVISFFSLFFSIMLISFQVKERQHQESLQLYQINEEYYHHL
                     EEFKHDYKSLLFSLKITLQENKKETLEFLEILEDHSAAIFEKPQIQQLTNIVSPAVRG
                     VFLKFLEKAESEKIPIQVVIPVEVKTIPIDLVVFIRCLSIFISNAFDHRVADQSPITI
                     NLLEEKEGSRFSIANKANPTNLTLSEMVKRGKTSKKNGGLGLYSAKKMLDVYENAELK
                     IEFSKQNHYFFVEMYLASRKSKSAY"
     gene            20897..21124
                     /locus_tag="IWO89_001207"
     CDS             20897..21124
                     /locus_tag="IWO89_001207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HAQ0455977.1"
                     /translation="MLTQQLRELEQDESIHRKAYNQVPPKVEYSLTEEGKSLREILVA
                     LSVWEEERIEKQKASREEDTLLHTHGGYLNY"
     gene            21247..21573
                     /locus_tag="IWO89_001208"
     CDS             21247..21573
                     /locus_tag="IWO89_001208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide pyrophosphohydrolase"
                     /protein_id="HAQ0455978.1"
                     /translation="MNSMEKVNQFRDERNWRQFHNEKDLAISISLEASELLELFQWKT
                     PEEVKETQLERIKEELADVLIYSYMMADNLHLDLDEIIEEKLIKNNLKYPVEKSAGVR
                     KKYTEL"
     gene            21641..22354
                     /locus_tag="IWO89_001209"
     CDS             21641..22354
                     /locus_tag="IWO89_001209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class A sortase"
                     /protein_id="HAQ0455979.1"
                     /translation="MKKKGKAKRWLVNILLFLLLLVGLALIFNEQIKDYFVRETGDKY
                     AIANVTKEDLKKNNDKDVSFDFDAVEPMTTEGVMRSQMRGTDLPVIASIAVPSVSINL
                     PVFKGLDNTSLLYGAGTLSPDQEMGKGNYALASHRATNPELLFTPLENLEMGAKIYLT
                     DLENVYTYKTFFKEKVAPTDTQLLNEVEGKEIVTLITCGDMDAVTRLVVQGELESVTS
                     IKDATDDMRSAFNLETKTF"
     gene            22664..23515
                     /locus_tag="IWO89_001210"
     CDS             22664..23515
                     /locus_tag="IWO89_001210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAQ0455980.1"
                     /translation="MKIDKILNNNVVISKNGFGEEVVCMGKGLAFQKKIGDEISPEAV
                     QKEFVLRDSFAKNQFQQLMADIPAEEIEWVKQVVELAEDKLKVEFSPNIYLTLTDHLH
                     YAITRAKENIQLPNPLLFETKKFYPKEYQAAKEALLFVKQKTGVELSEDEAGFIAFHF
                     VNSQQGNEDMQVTMTATTMVRDILSIISKFFGIVFDEESLNYQRIITHLQFFAQRYLK
                     GEGSDEQDEFLYALVQGKYPKAFHCVERINEYLRQTQKQEMGIAEQIYLTIHIQRVVS
                     EKKSIQS"
     gene            23636..25501
                     /locus_tag="IWO89_001211"
     CDS             23636..25501
                     /locus_tag="IWO89_001211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAQ0455981.1"
                     /translation="MDNQAVGRRVWEAVGGQKNVKSLVHCATRLRFRLKDESLADTQK
                     LKEDPDVIQVVQSGGQYQVVIGSNVADVYQSIVDEEGLAVSEDGKEESKNILNRLIDI
                     ISSIFTPFLGAMAAAGVLKGFLSLATVMGWLTADSGTYQILFAAADGVFTFLPVMLAF
                     TAAKKFKANQFLAVAIAMALVYPAITAVAGAGEAISFFGIPVILSPSGYTSSVIPIIL
                     AVWVQSKLEPFVKKVIPQFLQMILVPLVVLVVMVPLTFLALGPIGTVAGNALGGLFNS
                     IYGFSPIVAGLIMGSLWQVFVMFGMHWGFVPIMFLNIEQYGFDVLMPMLLPAILAQGG
                     AALAVALRTKDTKLRALGISSTVTSLFGITEPTVYGVTLPLKKPFIAACISGGIGGAI
                     IGFSGVKAFSSSLVSLLTIPTFINTVDGVESNVTVAVIATGIAFVLAFVGTLILGFDE
                     QTQDNQLENKHANAGKPITSARHTLKSPLTGKVLPLSEVPDQVFSSGVMGKGIAIDPE
                     VGELVAPADGEITTIFPTGHAVGITTTDGAEILIHIGMDTVELNGNGFEILVKQGDLV
                     KAGDLLIRFDIEAIRAAGYSVITPVVITNTDAFADILELDQKEIIANEDVLAIVK"
     gene            25549..26958
                     /locus_tag="IWO89_001212"
     CDS             25549..26958
                     /locus_tag="IWO89_001212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 1 glycosylhydrolase"
                     /protein_id="HAQ0455982.1"
                     /translation="MEQLLPNQLEGAYDRDGKGLSVADAMPGGKQRFAIIGSEEFDWT
                     IDQEKYIYPNHRGIDHYDRFKEDLALFAEMGFKCYRFSIAWTRIFPNGDEGTPNEAGL
                     EFYDQLIDECLKYDIEPVITISHYEMPLHLAKEYGGWKNRKLIDFYERFAQTVLERYS
                     SKVKYWMTFNEINSAFHFPALSQGLVKSNGAGEYQNIFQAWHNQFVASSKAVKIGHEL
                     RSDIQIGCMIIYATTYSIDANPVNQAATMIQNQEFNFFCTDVQVRGEYPAYTARTHKK
                     YGVDPEKLEQTEKDFQLLKEYPVDYIGFSYYMSTAINETDPQAATSEGNLLGGVKNPF
                     LEASEWGWQIDPEGLRIALNELYNRYQKPLFIVENGLGAIDHMEEDGTIIDDYRIDYL
                     RRHIEAMANAVADGVDLMGYTPWGCIDLVSASTGEMSKRYGFIYVDLDDEGNGTLNRS
                     KKKSFDWYKEVIASNGVNL"
     gene            27066..27767
                     /locus_tag="IWO89_001213"
     CDS             27066..27767
                     /locus_tag="IWO89_001213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAQ0455983.1"
                     /translation="MKRTRVLYLEVADKIKEDIFSGKYPVGSMLPTESELEELFQVSK
                     ITVRKAIELLASDEYVAKKSGRGTTVLSNRPYNRLSKAASFTQILENSRYEVRKETLD
                     LQLVHIKKEDPLFSFFGEQAVCFKRLYYLNNQPYIYFVHYLPQEMMQFSREEFGKESL
                     YRLLTKKGYTIDSFSDDFSAVFLTEEEQGILKTDAQIGIKRIRKSSTQIGEMIEYSEA
                     VYHTELHPYHIDYET"
BASE COUNT         9435 a         4521 c         5693 g         8124 t
ORIGIN      
        1 atgagcaatg atacacaacc agaaatccgt ttcccaggat ttacagatga ttgggaacag
       61 aggaagttgg gggacttcgt tgtagattat gttgaaaaga caagtgtaca aaatcaattt
      121 cctatgctaa cttcatctca acaaaaaggg atagttttgc aagaagacta ttttgcaaat
      181 cggcaagtaa ctacagaaaa taatattggt tactttgtac tacctagagg atatttcacg
      241 ttccgtagtc gaagtgataa tgatgtgttt gtgtttaacc gtaatgacat tattgataga
      301 gggattataa gctattttta tcctgtattt actttaaaat ctgctgattc cgacttcttc
      361 ttaagacgta ttaacaatgg aatccaacga caattatcta ttcaagcaga gggaacaggt
      421 caacatgttc tgtctctaaa aaagtttaaa aatatagtag caatgtttcc tagtgaggaa
      481 gaacaacaaa aaatcggcac cttcttcaaa caacttgacg acacgatcgc tcttcatcag
      541 cgtaagttag atttgctgaa ggaaacgaaa aaaggattct tacaaaaaat gttccctgaa
      601 aacggagcaa aagtgccaga aatccggttt cctggtttta cggaagattg ggaagagcgt
      661 aagttgggag aattaggtaa aactcaatcg ggaattggat ttcctgatgc cgagcaaggt
      721 ggttctgaag gtactccatt ttttaaagtt tccgacatga ataacattgg taatgagtat
      781 gaaatgagaa atgcaaatca ttatgtatct aatgagcaaa ttgagcgtaa aaaatggaag
      841 ccaataaaag atgtgccagc gattatattt gctaaagttg gcgcagcaat aatgcttaat
      901 agaaaacgtt tggtcacaag ccctttctta atagataaca ataccatggc ttatttattt
      961 aataatactt gggatatcta ttttggtaaa atattgtttg aaactataaa tttgccaaga
     1021 tactcccaag taggggcatt gccttcttat aacagttctg atattgagaa tatcagtgtt
     1081 aaggttcctg taaaggatga acaacaaaaa atcggtactt tcttcaaaca gctagatgat
     1141 acgatcgctc ttcatcaacg taagttagat ttactaaaag aaacgaaaaa aagcttctta
     1201 caaaagatgt ttgtctagga gggaagaaga atgaaaatta gtcaccgtga ggaagcagaa
     1261 gtcgaagaac agttgattcg tgtacttgga gaagggcata atcagtggac gtatcgtccg
     1321 gatctaaaat cagaagaaga cctttgggtc aatttacgcc aaaaaattat ctcaaataat
     1381 caagcagaac tgaatgattt tcctttgacc gataaagaat ttgaaacgat caaaacggaa
     1441 ttactgctgc gaacgaaaac cccatttgat gctgcgaaat ggttaaaagg tgaaaatggg
     1501 atggctcgga tcacgattga acgggaagat tcgcaattgg gttctgtctc acttatcctt
     1561 tattccaatc aagacattgg tggaggaatc tctacttatg aagtggtcca tcaaattgca
     1621 aaacgtggaa gtaacataga agcacgggat cgacgttttg acgtcacctt attaatcaat
     1681 ggattaccga ttgtccaaat cgagctgaaa caagtaactg caaaagatgg tgtttatcag
     1741 gcatttaacc aaatcaaaaa atatgcagaa gaaggtatgt ttcggaacaa tatcttttcg
     1801 accttacagt tgtttgtggt ttccaatgaa caaaccactc ggtattttgc aaatgcctta
     1861 ccgaaagact tgcaccctaa gtttttattt agttggcgaa cgaaagacaa tgaaaaagta
     1921 gaaaacttat atgagttttg taaacaagtg ttaaatatcc cggatgctca tcgtttgatt
     1981 gcggattata caatcgtcag tgaagatcaa gacaataaaa ccctcatggt gttacatcct
     2041 tatcaagtac atgcgattca agccttgttt attgccgcaa ataaacatca atcagggtat
     2101 gtgtggcatg cgactggttc aggaaagacg ttgactagtt ttgtatctac taagctttta
     2161 gcacgaaaat cggggatcga tcgaacgatc atgttagtgg accgcaaaga tttagataat
     2221 caaaccacca cagagtttac aaaatttgct tcggaattta atactgggat ttcttccggg
     2281 aacgccaaag cgaatagctt gatcgttgga acgggaagtg ccaaagaatt aagtgaaacc
     2341 ctgttggcag atgccaatgc aaatgttgta atcattacaa cccgtcaaaa actagatgcg
     2401 gcgttgaaat atgcaaagaa acaagaagag aaaaaaggaa ccaatcgctt ccaaaaatta
     2461 atgggccaac atatcgtgtt tgtagtagat gagtgtcatc gagcgttaag tgctgaaaat
     2521 atggaagaaa tcaaaaaaat gtttccgaaa tccacttggt ttggctttac aggtacaccg
     2581 atttttcctg agaatcggaa acaagccaaa gggcaattgg cacgtacaac ccatgatcag
     2641 tatggagagg tcttgcatac ctataccatt aagaatgcgc tagaagatgg ctctgtctta
     2701 ggatttcaag tagagcatga aaacaccgtg gagccgactt cactagagaa taaaatctat
     2761 cggaaattaa aagaagtaga aacatatgca gagtattcat cagaacagat caatcgtatg
     2821 attgatcaaa tggaacctgt caaaaaagaa agttatttgg acccagctgt ttttgaagca
     2881 gatgaacata tccaaaaagt cattcataaa atgttccgtc cagataatgc ctatacaaaa
     2941 tttgattttc gaaatggcag gccaacgaaa tctgcaatct taaccacgag ttccattgac
     3001 atggccaaaa agtattatcg ggccatcaaa gagatgacga aagagcctga ttggttaaca
     3061 aaagagtttc ccaatcagct cattcgtgaa ggacgaacga tggaggatcc tgattttcca
     3121 cgaatcgcta ttacctattc attagaagaa aatagccgag attcaagtgc ccaacaagaa
     3181 gaaatgcaaa aaattattga ggaatataat cagtattatg atacttcctg gtcattagca
     3241 gacattgagc gttataacgg agacatcaat aatcgtttgg cacgaaaaag agcggaattc
     3301 aaacaatttg ggaaacaaat cgatttagcg attgtagtgg atcgtctatt aacaggtttt
     3361 gacgccccta cgattcaaac cctatttgtg gatcgtaatc tagagtatgc aggattgatc
     3421 caagcttttt cacgtacgaa tcgtacgtat cctgataaga caaaaggatt gattgtgacg
     3481 tttcgtaagc cagcgacaat ggagaagaat gtcgaggatg cgacaaaact gtattcaaaa
     3541 gcaaaagaag aatcaggttt gatttatcca agttatcagg aatccaaaaa aagattcaaa
     3601 caagcatatg gtaagttaaa agaggtttca tccatggaat ccattgatga acatacgcca
     3661 cttgaaatcc gtatcgagta cgtcaaagca tttcaagaat tgaatcgtgc ttatgaatcg
     3721 ttagtgactt atgacgatta caatgatgac atggtgaaat ctccaacttt gaacaatcaa
     3781 attcaacttt tggaagaaca aattggcgtg tacgagaccg tgaagggttc attaatagag
     3841 gaagaaccag aaaaagaagg agaagatttt tcaacgatcg aattttatag tgaaaatacc
     3901 actaagttgt atgatattga ttcaacttat attgatcaat tactaggaac ttatgctgcg
     3961 aatagttcag atgcacgaga agaaattgaa aaagctttgg ctaaattgaa taaaacagaa
     4021 ggagtcaaag aagtttaccg acaaatttta aatgcatttg atgtggggac tttagatagt
     4081 aatgaagata tctttgtcat taaacgtcgc tattttacac aagcgagtga tgatttgatt
     4141 catgtctttt ccaatgagtg gtttgtttct gagaaagaat tacatgcttc taatctgcaa
     4201 tatctaccag gtgaagatcc aattcctaat atgaaagcaa ttatcaacag taaggattac
     4261 gaaggatata aagccaaaca tccagaagcg aagccattta aatacccaca agaaatgaag
     4321 cgagcatggc gtaaaatgtt agatgatgag ttgattccac ttgagaatga attgaggtaa
     4381 acgagaagag ttaagaaaat tgtacgattc aaagatggta gtcaagtgga agtgtagaaa
     4441 atagggatag atgtctttta tttgttcaca ttttataacg aagtatttaa gacagctatt
     4501 gaataatgtt tcctccacct ttttcattca tttaccccac cccgttgtta tatgaccgtg
     4561 ttttgagagg aaccaataaa aagcataggc taactagggg ttcctttttc ttttgttttt
     4621 ttggaatccg cttaagtgaa atgtatctga aatgaaacgg agataaagtg attttttgct
     4681 ataatgacaa ggttttagct gtttcagata ccatgtaacc caaaatagtt tattgagaaa
     4741 ataggagagt atttgatact aactagtagg aaaaaatgag ataaaaatta aatggaatca
     4801 tttcaggtaa tctcaaataa aaaaatatac agtcctgtgg cactttacct atgggactgt
     4861 atatttttta ctctcagcgg gcttgttttg actccgtgtc tgactccgtc gtattgattt
     4921 taaatgatat tcgatgacca tttaacaggt catttttgaa atttttgtct ctatatgact
     4981 cagtttgata attattgata gatcaatttg ggtctatatc gaacaaggga ataatccaat
     5041 aaaaaatgat ttagaatatc aaaataacaa aaaagaggac tacctgtatt cgtcctcttt
     5101 tttgaccaga ttttgaccag tctaatttac attctaatat attcatatga gctaattttt
     5161 tgattaaatg gtaagttttt ccttgtttgt tttagtttaa actagtgtgt aatttaaaaa
     5221 tctatatgga agggtctgtg gggtaatact taaataaagg ctaggaacct gataacatca
     5281 aggttcctag cctttttata ataaaatgaa gcgaaaataa tttcttttaa agagccgaga
     5341 ttatcagcta tataagagtg aatttgatgc aaaaaacaga gccgaaacag tcttggtgtt
     5401 cgtagctctg cttatatgtt tcgaatttaa gacaaagagt tggcaaaaga tgttgataag
     5461 gaagataaga taactgtgtt atataaacta ggtaactaac tgtaacaagc taattttaag
     5521 agattcttat tagaagacgt ttaggaatat aactaagaaa atgattatac aggtaattgt
     5581 catttcaata atgttttcta attttcgcgt ctttgtttta agaaaaaggc cagaatcagt
     5641 tggaaaaaag ttcgtacgat agtgaagagg ggcaaaattt cctactaata ttcccactaa
     5701 caagagataa aacggccagg ttggtgtgat tcctctataa aaaaatgccc atgctaccga
     5761 aatcccatag attaacaact gaacaatttt tacaatgaaa aaattgttct gccattactc
     5821 aaagtctctt cctccttgta tcgtttattt gtttcatttt atcattatga taggcgcttg
     5881 aacaatccat tttgtatttt attgagtact cttggataaa ctaccagcga ttatcatatt
     5941 tatttatgtt actgtaagcc atcatataaa agtaaatgag cagatcatag aaatataaaa
     6001 aagaactaaa attgatctaa attatgtgca gcttgactac gtaccaatct atattactta
     6061 ctagattggt cgttaatcta ttcggatcga atgagcaaaa tatttttgtg aatgtccatc
     6121 cttgattatg attccaaaaa gaatcattgg gaggctgtgg cgtaaatatt atactataag
     6181 ccagaaatac ctatcattct gaactttttt atttaggaac tttcatctca aggctttttc
     6241 aattaatttg tacccctgtt ttttggataa gatcatgtgc attgattttt tctatctcta
     6301 cgttcatctc ctacttaatg cctatctggc tcctgagggc ttctttttaa ggcgtcgtca
     6361 tagtcttggc tgaggatttc ttggtaatct gagggaatgg cttcccaagt gagcaggcga
     6421 acgttcatga cgtgttttcc tttgtgtttg attacagcat ggtttttctc tttttctacc
     6481 tctccagcgt gaaaagacag ccatgtacca tggccaaaga tatcattaca tacccaacgt
     6541 tcgatggaat tgtccacgta tcctacaggg agtgtggtat agccgatttg tggtttcaaa
     6601 aaaggattga atcgatcgat cgtttcattt ttgtattgat aattgataag aaaaagatag
     6661 accaaacaaa agacgagcag aaagaaagat agtgtctttt tgagtgagaa gggttgtttt
     6721 gatgaataag atttcttgtt aacaagaggt tcatctggca atgttggttc tattgattcg
     6781 gtagctgatt caatagaagg aaaatcaatc ggcagaccta aagtatgatg taaaagtgac
     6841 agcatttcaa ggtctggcat tgtacgagaa ttttcccaac tagaaatcgt tttatcggaa
     6901 acaccaattt tttcgcctaa ttctttttgc gtaagcttac aatttctgcg gtaagttcgt
     6961 attttttctc cggtgttcat cttcttctcc tttcgattct acagtatata gatttattct
     7021 acatgaccta tacacttgat agatacgttt ttttgatgaa aaacggtatg attttatcaa
     7081 agaatcattt aagtaaggga gagtggtgat aatcaaaaaa aagttcacag ttttactcac
     7141 aatcatagtg accggtatca tgagtggttt tgccttggtt agtataaata aacaggtact
     7201 ctatcggaca aatgaatttt tccatcaaac agatacttat actccgattc gattgccaca
     7261 acattttatt gggcaagaag aatatgagca gctaatcatg gtatttgaaa atgtctcatc
     7321 agaaactgag ctttcttata tgaaaaaaac agtaaatgaa tccaatgaag tagatacatc
     7381 ttggaacttc aagatacagc caaagcttga aatgacattg tattatcaag gggatgtcaa
     7441 atcttctcac gagaaggtac cctattctaa tacctccttt cgcgtaacgt ccattgaaga
     7501 atcgatcaaa ataaaagatt ttgaaggtga atttttcttg atgacgagag aaaaagaagc
     7561 ctatgaacgg ttcatttcac tattcactga tcgattcaat caagcatttc acactgatta
     7621 tgccactaat atttttgaag actttgaaaa tagctattcg tattcggtta tttcaaatac
     7681 agacgtatat aattatcgag tgtatttaac tattggattg attcttttat tccttttgtt
     7741 atcatccttt ttcttattga tgaataaaga aatcagttta ttgttgaata atgacttttc
     7801 attacatcaa gtattgtggt gtttattggg gaaaaagaaa atcattatta cattcataat
     7861 tgcaggaatc attggtggtt ttatttttat gtattataag gagcaatttc tttatttgtt
     7921 ccagatgtac gttttcttgt tcagcggaat atatggtatc tctttgattt ctcttttatt
     7981 agttgaaaaa atcgcttcgt taagagaagc gttcaactat atccaataca ttctttttac
     8041 atttggattg ttgttttctc ttcttttgac cgttgccaat gtcgaattag caactatcgt
     8101 tttttcttct tcacagttag ctgtttccca tgaaccacca aaagaaatcg ctggtgagga
     8161 ctatcatgta ttttatccag taaccatcgg gaaaaaccac gtggatttta tttattccaa
     8221 tcgttttgca tcagcagcag atcaggaatt atatgaaact ttgaataaaa acggaagtat
     8281 attagtcaat gtatcagatt acttgaatga agaaaatgaa cagtttggca gagggataaa
     8341 aataaatctg aattatctta agaaatttcc tttgattgat gtctctggaa ggttgatcca
     8401 aataaccgaa aaagaaacgc accctgttat attgatccct gaacgatata gacatacaga
     8461 tttaaaaaat gacctggcgc aaataacaga atattatcaa gaaatcagtg aaaaagaacc
     8521 gaaatttcta tttataaaaa acaatcaacc catttataca tttattcctt ctatcccctg
     8581 gatcgaacat tatccggttg ttgaaatact gacattgaaa aacagtatgt atttggaaag
     8641 aaatattctc agcggagaaa tctatccacc gttaaagatc aagataggct ctttgtcgaa
     8701 agaaaggtta tttgagctga ttgaagaaag tcagttacgt gataacttgc agttatcatt
     8761 tccgtatact cagactgaag agatagctgt caaagtgtta agcagatcat ttcattactt
     8821 gatacagata tttttactca ctttcttttc tttcttcttg ttgagctatg tcatgttatg
     8881 catatatttt gtctacaatt gtcggaaaat cgctatcttt cgttcaagtg gttattcgct
     8941 ctttgaaacg tataaagatt tcttcatgat gaacctcatc aaatggggaa cgacaagtgt
     9001 aatcttcctt tttcttatag aaagagagcc taaatatctt tttaatattt ttttctttag
     9061 cttcattgat tttattcttt cagttgtttt tattttgtct acagaaaaga aaagtcagtt
     9121 attgctgatg aatggaggtt aacaattgga gccgttgata gtgataaaag gtgcgaaaaa
     9181 aatcattcga aacaaaacga ttcttcattt ggatcgtttc actgcggatg ctggggatct
     9241 tgtgactgtt gttggagaaa gtggagcagg taaaagtacg ttattgaata tattaggatt
     9301 gaatgatcag ttcagttcag gacaattcta tttgttcgat caagagacga aacaattaac
     9361 tgggagagag aagcgcttga taaaaagaaa taccatatct tttctttttc aagattttgg
     9421 gttgattgaa tatgaaacaa tcgattttaa tttagatatt ggattgaagt tttcgaattt
     9481 aaatgtttca gataagcgaa cagcaaagta tgaggcattg aaaaaagtaa atataaacaa
     9541 gaagctaagt acgcctatct acacattatc tggtggtgag aagcaaagaa tcgcctgtgc
     9601 gagagtctta ttgaagaata gcaaaatcat tttagccgat gaaccgactg ggtcattgga
     9661 taaaagaaat aaggataaag tagttgaaat cttatcgcaa caagcttccc aaggtacgtt
     9721 agttatcgta gtcacgcatg atccatatct gaccaaaata ggaaacaaga agatcatgct
     9781 ttaaaaatca aaaaggaaag gatgtcgttt tatgaaaaaa gcagttatta tttgtgcact
     9841 agggataggt tttgttggag gaaacgctat gtcatctgaa gtggaagcaa attcagttca
     9901 cggtgaaatc tattacaaat ctttaaagcc aggagagatt cattttgatg ctaaaggtaa
     9961 tattattaag tcaaatttac cagtagaccg ggaaatttct agaacagcaa ttaatcatag
    10021 ctctggaaga tgggtttatt atagtgatac aacttgtacg agaaaaacag gtcattctaa
    10081 ccactatagt cgtgtctata atcgtcacgg tgcaaaagcg aaagttggaa actctattcg
    10141 atcagcaaat gcaacaaaag gagtatgggc ctatgcaagt gcgacaggaa atagaactga
    10201 cacattctca tgttggtaca atccaaccgg ttggtattaa gtatccaaaa gtttatataa
    10261 aatagaaaaa acatataatg aaacagcgtt aaaagttatt tttggtagtt ttaatatatt
    10321 catttttagt tgtataaaaa tatatcataa taaaaacaac aaaaaaatgt ctggaaaatc
    10381 gaacataaaa gtgtctgatt tgaatatccc agcagcggaa gaagaaaaac aaaatcgata
    10441 acatttgttc taagaattag ttacgttaaa tacggaacgc aagatagact tgaaaaacgg
    10501 taacaaaaga agtagtatat taaggtgtga tgtgctactt gacatttatt ctgctgagcg
    10561 aactttctgg attgtgtagc cttatatcta ttcaggacgt atcgagcaga ttagtaaagg
    10621 tgttcttgat gattttcatc ttgaacacct ttgtttttag caattcgcaa aaaacgaaaa
    10681 gtaggagaat tgataaatga aaaaaagatt tttagcactt gccatcgtac taggcactgg
    10741 cttattgtca ggctgtacga atgccggtga aaaaacagcc gttagttata aaggcggtac
    10801 aatcagcgaa caagaagtaa tggatagctt gaaaaaaatg caaggagcag attccgctgt
    10861 gcaacagttg atcgtttatc aagtatttga agataaatat ggagatgacg tatcaaccaa
    10921 agaaatcgat tctcaatatg atcaaacaaa gaaacaattg ggtgattcat ttgattcaca
    10981 attgaaatct gcaggttaca cagaacaaac attcaaagac agtatcaaac aaagtctagc
    11041 ctttcaagaa ggtctaaaga aacatatcaa attgaccgat gaagatttaa aaacggcttg
    11101 ggaatctttc catccagaag tggaagctca aattatccaa gtagcaagtg aagatgatgc
    11161 aaaagatgtg aagaaagctg ctgataaggg agacgatttc tctaaattag ccaaagacaa
    11221 gtcaactgat actacgacaa aagaagatgg cggaaaagtg aaatttgatt ccactactac
    11281 aacggttccg gcagaagtca aagaagcagc ctttaagttg aaagatggtc aagtttctga
    11341 tgtgatcact tctaccaatg cttcaactta tactacggaa tattatgtgg ttaaaatggt
    11401 gaaaaaccaa aacaaaggca acgacatgga taaatacaaa aaagaattga aagaaattgc
    11461 aacagacaca aaattaagtg acagtacctt ccagaataaa gtaattggcg aagtattgaa
    11521 agacgctaat gtaaaaatca aagacaaaga ctttgaaaat gttttgagta cattcacttc
    11581 agattctagt acagcaagta gtaaataagc ggtataaata aaaaatatga actatttaaa
    11641 tcaataaaac tgttcatcta aatcttttta ttagaactaa atcaacagta gtttaattac
    11701 tacttgattt agttcttttt attttaaaaa ttttttttac ttagaatcca gcgaccctag
    11761 atatttttct gtataatctt aaataaagaa aacaatatca aaaaatgcac gtagaagtag
    11821 ataaatggag tggttttgca aatttctttt atttctagaa gttattcacg tatacggatt
    11881 tgtttttctc acaaataaaa aggcaggaag tacaatggat catttgtata taaaatatat
    11941 tcatcaattt tccgttttgg tgttaggcac actaattata gcggggattg tcgcatttat
    12001 tattcaaggc cgtgacatat taacagtaca tcaagagttt ttcagttatg catttggtat
    12061 tcctctgatc gctataaggt ctgataacaa aaagtggaag aaacgctatt acattctctt
    12121 gctatttttg tgtttttatc tgccacctaa gatttttact ttattaggta tacaatgcct
    12181 atcattttct tttatttcct atatcattgt tttaattttc ctcagtttca tagtatggat
    12241 gttttatctt gtcaaaaaat aaatgaatgt ttcatgaaca gtacttcatc acagcctaat
    12301 ttttttcttt atgctatcgt tatattaaag aaatcaatca gaagggaaag aaagtgatga
    12361 aaaaagtttt attagtggta aaccccagtt caggcggtga acaagcaaag gaatttgagc
    12421 aattagcgat agcaaaattg gaatcggtgt ttgatgaagt ggtcgtcttg catacaaaaa
    12481 aagcagggga tgcaaaaaac tttactcgcg aagcggctac ggaagggtat catagtgtgt
    12541 ttgtcatggg cggagatgga acagtcaacg aaggaatcag tggaatcgct gaacaagaac
    12601 atcggccaaa tttcggattt ttcccattgg gtacggtgaa tgacttggct cgagcattag
    12661 ggataccgct agaacctgag gaagcgatca atcatttttc gattgaatca gtcaagccat
    12721 tggatattgg aaaaataaat gacgactatt ttatgaatgt cgtcgcaatt ggctctatcc
    12781 ctgaatcaat caatgatgta gatgcggaga agaagacgaa atttggaaaa ctagcttatt
    12841 tcatgtcagg aatcaaacag ttagcttcca cacaatccta ctcgtttcac gtagaagttg
    12901 atggaaagaa ggaagaaatt gagagcagta ccttgctgat aggactaact aactcagtcg
    12961 gcggttttga aacattactt ccaaatgcaa aagtggatga tggtaagctc catttggttt
    13021 atttaaaaga ttcttctttg atcgatacgc ttaaaacatt gccagaccta ttaaaaggag
    13081 tagatgaatc tacgaaaaat ctagcctacc ttacttgtga gcaattagtt gtgtcattag
    13141 ctgatgagga agaattgacg acgaatgttg atggagatga aggggataaa ttacctgtga
    13201 caatcaaagt attgccctca catttacagg tttattgtta aaatggaggt tgtgaccgag
    13261 gagttggcgg gagaacaccg cttgttcggg ataagggact gtgacaagac ttttgtcaca
    13321 gccttttttt tgtacaaaaa agaaatttac acctaataga aaaatttttc cataaaaata
    13381 catattttaa atatgaaagt acaattggaa atagattaaa acgataaaat aaaattgtca
    13441 aaacgtcaga gaggatggtg gagaatgaat caatgtgtaa aaaagctgat tgcgtcgatt
    13501 ttgtttgtct tccctctctt gatggtgaca tccgtttttg ccgatgtaga gaaaacaaac
    13561 gaatcaacgc aatcagagaa tcaaagtttt gacaagacta ctttttcaga agaatcgatt
    13621 acttctgaaa ctacagaatc tcaaagtgag gcttctacta ataaaaggca agaagaaaat
    13681 aagacaagtg agacctcaga agtagctgaa caaacgtctg atagcagtga agcggcactg
    13741 gcaagagctg tttcagcgga tgggtacagc gaagctgcca caacagaaga actggcgcag
    13801 cttctcgcag acgaatcagt aactaagatc cgactgattc agcctttgac tttagacagg
    13861 gaattagaga tcaaacgaga tatcgttatt gattttggtg gattcgctca caatttcggc
    13921 acacaccata tttatatcaa tgagatacct cagtcgatcg agttccagaa ttttaaagga
    13981 acagctgcac atccaggtgg attgacacca aatatcgatg gaaatgccat tatcattgct
    14041 tatatgaccg atttttgggg aagcagatat cattttacgg gagaaataaa atttaccggg
    14101 acacttgatt tgtatgatgg gtccaaatta ggactgattt acgctcctcg tgcaactgtc
    14161 acattagatg gtgtttccgg tgtgttggat gtccaaccag tgaaggaagg actgaatgcc
    14221 gctccgggta aagcctattt ttgtagaagc tatcaattga atgtgatcaa tggtagtcaa
    14281 ctttatggac cgtatctagg gaaattctac ggttttctag attcaggaga tgagactggt
    14341 agtggaaaaa cagcgccagg aatccatatc ttaagcggtt caaaggtctc tcttgattat
    14401 gatcgggcag atggcgttag cgatgaagga gaggcagtcg atacgctacc tggtaatgtc
    14461 atgtttgaag tcagtggttc gggaagcgaa ttttcggtaa atacaaagat caatattacc
    14521 aatgacaaca atcgcggaat catccaaatg agaggttcag gatcgaaagt cactgtatcg
    14581 aatggtggga aaatcaccat caatacagca actacgggcg gatttcgctt gcaaggagat
    14641 tctagtcaaa tccatgtatc aagtggtgga caaatctcgg tcaaaatggc tggagatgac
    14701 gatcaacccg ggaacaatgg gatgcgtttt gtaggaaaag gcctttcttt gatcgtagaa
    14761 ggagccggat cagttatcga catcgataag cagagtgggc gttccagcgc agttcgtttt
    14821 gagaatggta cgcaaaagct gacagtaacc aatggaggct cattgagggt aagaaataca
    14881 ggtgatggca aatcttacgt gaacagactc aatcaaggaa atcaagcgat ccaattttat
    14941 gacgcttctg gttttctaga aagtccaggt tcagctgatt ttacagtcac tggagaaaaa
    15001 tcaaatatcg atatacgcgc agattccgga gcaactgtag atacaactgg agatattgat
    15061 ttaaatttta cggcaggtga aaaaacttat ttagtcatgg tcgggaaaac aggggaagat
    15121 aagaccggca tcatcagtgg ggatgtgttg aatgtggact tagaatctcc tacctatttt
    15181 gatttccaaa acaaacggat tggaagcgag accaatggca taggcggctg ggtattccaa
    15241 ggaaatggag attcttcttt aaagatcgcc cattcagaaa tcgctttatg gcgaaaaaca
    15301 ggcgcaacat tcgataactt tgaaaaagac ccggattatt tttctgatat gacagactta
    15361 tcgatcaatg ggaccgactt aggcaatttc gtatcaagcc ctagtgcagg tttaacaaat
    15421 gaatttgctc agacaggtgc cggaatgaaa aattataatc gaatcagtgc caataatcag
    15481 ctcccgatga ttgaatcctt aagagtgcca acgaatgcag ataaaaaaat ctatgggcat
    15541 gtcgttgttc ccgaaggtgt cgaggcaaag ccaagagatg cttggacagg cgaagtagaa
    15601 gtgactttac gtattactta cgcagatacg acgaaatcat ctgtagaaat tactgctctc
    15661 acgcaaggac aaacagatga aaatgaaggg gcaggatata acccatgggg agaaggcgaa
    15721 caaggcggct tgttcgaagt tcaagtaccc aatgatgaga gattaaaaac ggaggatcaa
    15781 gtgcaagttg ttgctgctaa aaaagtaaga ggtggccaag aagtgacaga actggactct
    15841 gccaaaacga cagtcgatgt gattcctcct gaacctgcta atttaagtac cacattgatc
    15901 aatgcagctt ctcgcacgat cacaggtaca ggtgaagcag gagccttggt ttcattgaaa
    15961 aaaaatcaag ctgttttgtc agaaacaact attgatgata caggggaatt tgttctttct
    16021 atcccagaaa atcaactttt tgcaggagat gtattagaaa ttgtattaaa cgaccaagca
    16081 ggagaagcta ctgctaaggg tgtgcagaac aagccaagta ccaacgatga aattgggaat
    16141 cacaatccaa gtgatacgcc agttgtttat catgatgccc cgccttttgc acctgctacg
    16201 aagatcgtag tagaaggtgg tctctcattt cttgcccctt ccaaactcga ttttggtcag
    16261 atcaaagcaa ctggtttgac acaagaagca tttggccaaa tggatataca ggaaaaatta
    16321 tggatttatg atcagcgaga aacgaaaaat ccgtggatct taacgataaa attatctcag
    16381 cctttcacaa atgatgcagg attttctatg caggatgtcc tatatttcaa gaaaggagag
    16441 gagtatcaat tagtgaatga agaagaactg gaaattgtca gaggacaaaa ttcaacgaat
    16501 gtgcaagcag attattcaga atatttaaat aacggtcggg cgtttaaact tgtcttaacc
    16561 aaagaaaatc agatagctgg taattactca gcagagataa catggacatt agcagatgcg
    16621 ccgacagaat aaagatcaag gaggatgatt gatgaatcga atatggaata aaagtcatcg
    16681 aaacaactgc tctttactta tgatccttct ccttggattc atcctttggc agacacctgt
    16741 ccatgcctcc gaattcaatt ttgcggtaga ttttgaatta ccggctaatc agattgataa
    16801 gaacaaaaca tatataaatg taaggatgga gcctgggaaa acacaggaaa tcccctacac
    16861 ggtctttaac cgaacagata aatcagtaac agttgagaca ttgatccact ccgctacgac
    16921 gaattcaaac ggtgtgatcg aatatggaga atcaaaaagt aaatttgatg aaacattaag
    16981 ttatgatatg aaaaacttag caaaaacagt gggagaaata actgttcctc ctcaaagtgc
    17041 ggtcactgag acactttcac tgaaagcgcc agaaaaagag ctggctggtg tggtcgcagg
    17101 agggatcacg tttcaagaaa aaaaagagaa cgtttctaac aaaagtacag ttggtgtcgt
    17161 caatcagttt tctcaagtga aagcaatctt gatttgaaat agcgaaaaag agtcttcccc
    17221 agatttgaaa ttaaaaaaag cttatgcgga tcaaagcgat ggaagaaatg tgattaaggc
    17281 gaatcttcaa aatccaaatg cttcttatat gttcggcttg actacatcgg ttgaaatcta
    17341 caaaaaagaa acaaaagagc cttacctttc aataaaaaaa gaaaaaatag atgtagcacc
    17401 caatacaaat tttgatttac ttgtcccatt agaaggaaaa aaattagctc cgggacatta
    17461 tcaagcggtt gtgaagggtg cttggaaaga aaaacgctgg aatctccata ccgattttga
    17521 aataactgat gatgcagcgg accaattgaa tgaaaaggat attgatttaa aaaatcagcc
    17581 agaaacattt gactggcgat ggctgatact tgttggagtc attcttattg gcttatttgc
    17641 tggattattt attttaaaag aaagaaggaa aaagaaatga aaaaaacaat tgcattaagt
    17701 accgttctag ctggaagtct attattatgt acacaaagtg tttttgctga gaataagagc
    17761 tcagccacta cagaaggaac gatttctgtg accaaaccag atgctgcaaa tcctgaagac
    17821 gttgttccag ttcctcctgt tgatccaaat gatcctgata ctccttttac acctgacgaa
    17881 ccaaatcagg gaacatccgg attactgacg attgaccaag ctccagatct tgattttgga
    17941 acaatcaagt taggtacagc aaaaacagta tatgctgcat tacaaagcgg aacggacagt
    18001 actggagcac aaacacaagt acagaatttc gtacaagtaa cggataaaag cggaaattat
    18061 gccggttgga aattatcagt caaacgaaca gaatttattg gtacaacatc tgatacgaat
    18121 aaactaactg gttcaaaggt gaaattcaaa aacgccgtga ttcgaaatag cgcaactaat
    18181 agcagtggaa tgccatctac cgtttctgga actggcgctg aaggaatcga aatcccagca
    18241 gatcaggcag tagatctagt aactgcagga gcaaatgaag ggattggcac atggatttat
    18301 tcattaggag agaatgtgga acaagggaga caatcagtgg aattatttgt tcctgtatcc
    18361 aattatgctg tagacaacta cgtttcaaca ttcacatggt cgttaacaga tgctcctaca
    18421 gaagaggaag cgtaaaatca tatcaacgat ttcagggagt ggagccgatt caaggcccac
    18481 tccctgaaat ctgctatgct tacgtctcag ctcttattca tcaactcgaa gcagtttttt
    18541 ggaagtcatg gaaacgttcc tttgtctttt caaagatctt tcgaccaacg agtaaggagt
    18601 caccgttgaa aaattctaac acataatgag cagaatcaat tttctgcaat tgcgataagt
    18661 tgatacagta agattttaaa agtaaaaagt aagcatactt ttctaactct gtcttcaatt
    18721 gttttaaaga agtattcact aatttatctt ctgtagttgt tttgaccata acttggtttt
    18781 tcaaaccttt gatactttca atatataaaa tttcccgagg atacaggacg agacttccac
    18841 ctttttgatc aataaagatt ttatcgacat ttacgatctg ctgttctttc caattgatct
    18901 gttctagcac ttgacgaatt tgttcagata aattcctgtc ttttctgata tatccaatcg
    18961 gaaggatttt ccgattgatg atctctactg caaaattcgt ataagcggtg aaaaaaacaa
    19021 tattactatg tgcatttttt ttacgtattt gttcggcgac atcaatacct gtgagtgatt
    19081 gcttcaagtc gatatcgata aagaaaacat cgtgattatc aatagtgccc aactggatca
    19141 actgattgat ttgagagata gaagtgagct gcacgatatt ctcgatatga tgattgacca
    19201 attctctgat gatccattcg ctgtgaaact ggttatcttc tatgataaaa tactttttca
    19261 tatgcaggct cctattaaaa aaatatgtgt acattttgaa acaaaacaat ggaggaataa
    19321 gatggtcgca acagtgacac tttatttgac ctttactttg attttagatt taggtatcta
    19381 tttattcttt tcgaaacaac tgctagtaaa aaacagcttt ggttgtatag ctttgatggt
    19441 ggtgacactt ttacttccta tccctaaagt gagctttcct tacctagttg gcttattatt
    19501 gatgtttctt ttttatttta ctacgaaaaa cctgtttatc agctgtttga tcagtaatct
    19561 tttttttgct atcactggac tcagcttttt tctggttttt gatctgatgg attatttagg
    19621 aaagagacat cttggaacag caagtttttt tgcactactt atcgcattta ttttctatct
    19681 attagtaaaa agtatagaca aacagacgag atttctcaac ttgcttttta cttatccaac
    19741 tgaacactat tttggaggta tttgtatttt attaagctgg ggcgtgattt acttggtatt
    19801 gggaagtgtt cacttccgct catctaatta tctgcttcta tctcttttgt cgatagtcat
    19861 cagttttttt tctttgtttt tttccattat gttgatcagt ttccaagtaa aagaaagaca
    19921 acatcaggaa tcgcttcaac tgtatcaaat aaatgaagaa tattaccatc atctagagga
    19981 attcaaacat gattataaaa gtctactgtt ttcattaaaa attactcttc aggaaaataa
    20041 aaaagaaacg ttggaatttt tagaaatatt agaagatcat tctgcagcta tttttgaaaa
    20101 accgcaaata caacagctaa caaatattgt atccccagct gtcaggggtg tctttttaaa
    20161 atttctagaa aaagcagaat cggagaaaat cccgatccaa gtagttattc ctgtcgaagt
    20221 gaaaacaatc ccgattgatc tggttgtttt tattcgctgt ttatctattt ttatttctaa
    20281 cgcattcgat catcgtgtag ccgatcagag tccgattacg atcaatctgc tggaagaaaa
    20341 agagggatca cgttttagta tagcgaacaa ggcaaatcca acgaatttga cgctatcgga
    20401 gatggtcaaa agaggaaaaa cctctaaaaa aaatggcggg ttaggtctat acagtgcgaa
    20461 aaaaatgttg gatgtttacg aaaatgcaga actaaaaatc gaattttcaa aacagaatca
    20521 ctattttttt gtagaaatgt atctggcatc tagaaagagc aaatcagcct actgacttac
    20581 tgtcaaagga taagtttgag gaaagtctac acggagatag agacaacaac acagcatatt
    20641 caaaagaaac agaagcgtga tgaccgttgt cacccttctg tttcttttgc tatgtagtta
    20701 aacaaagtag ttgacacttc cgctacttag tattagtacg aaagtatact tcatgatact
    20761 aagtagataa gaagtgaaaa gcggaaccca cgatcgtcaa aatgctaact gctttgctta
    20821 caccagataa agggaagagc aatatcgctt attttaggcg tggagaagca agcaaatccc
    20881 tacgatcaca aaaaaaatgc tgacccagca gttaagagag ttagaacaag atgaaagtat
    20941 ccatcgaaaa gcctataacc aagtcccgcc taaagtagag tactcgctca ccgaagaagg
    21001 aaaatcatta agagagattc tagtagcgtt gtctgtttgg gaagaagaac gtatcgaaaa
    21061 acaaaaagcc tcaagggaag aagacacgct actgcataca catggtggct acctaaatta
    21121 ttgattttct atgacattta ttttaaataa aagaaaacag gagttttttt aaaagcatca
    21181 tctatttttt atgtaaaaaa atggtatgat taatcaagta agaagtgatt ggaagaaggc
    21241 aagcaaatga atagtatgga aaaagtcaac cagtttagag atgaacgtaa ttggcgacaa
    21301 tttcataacg aaaaagattt ggctatctct atttctttgg aagcatctga attgttagaa
    21361 ctttttcaat ggaaaacgcc ggaagaagta aaagaaacac aattagaacg aatcaaagaa
    21421 gaactggcag atgtattgat ctattcatac atgatggcag acaaccttca cctggatcta
    21481 gatgagatca ttgaagaaaa attgatcaaa aataatttga aatatcctgt agaaaaaagc
    21541 gcaggtgtac gaaaaaaata cacagagttg tagaaatgtg tcattcggct gttggaaaga
    21601 gcaaaagaaa aacagtaaaa tgagtctgga gggagagaaa atgaaaaaaa aaggaaaagc
    21661 aaaaagatgg ctagtcaata tcctcttgtt cctcttgtta ttagtaggac tagctttgat
    21721 tttcaatgaa cagatcaagg attattttgt cagagagaca ggagataaat atgctatagc
    21781 gaatgtaaca aaagaagatc tgaaaaagaa taacgataaa gatgtcagtt ttgactttga
    21841 tgcggtagaa ccaatgacga cagaaggggt catgaggtcg caaatgagag gaacagatct
    21901 accagtgatt gctagtatcg cagtcccttc agtttcaatc aatttaccgg ttttcaaagg
    21961 cttggataat acgtcactac tttacggagc aggaacatta tctccagatc aagaaatggg
    22021 aaaaggaaat tatgccttag caagccatcg tgcaacgaac cctgaattgc tgtttacacc
    22081 gctagaaaat ttagagatgg gtgccaagat ctatttgaca gatttagaaa atgtgtatac
    22141 atacaaaaca ttttttaaag aaaaagttgc tcctacagat acccaattac tgaatgaagt
    22201 cgagggaaaa gaaattgtca cactaattac ttgtggcgac atggacgccg tcacgcgact
    22261 agttgtacaa ggagaactgg aaagtgttac atcgataaaa gatgcgacag atgatatgag
    22321 atctgctttc aatctagaga caaaaacatt ttaggatact gggacataag tcgttacaga
    22381 ttaaaaacag ccaaaggata tgaatcgata aggaaaaacc aatttgctga aaaaaagaga
    22441 aacgcaaaga ggtgcatctc tgatttggtt gttttctaga tagacttgtg ttccagtttt
    22501 tttatttttt atatgaaaag gcttgcataa ataaaaattc gatggtatga tgattttgaa
    22561 taagaaatgg attgttactg tacggcaggc aaaaccagaa ttcagctaag aaacagtagg
    22621 cgaattctag gtttttttat ttttcttgag agggcgaagc aaaatgaaaa ttgataaaat
    22681 tttgaataac aatgtcgtca tttcgaaaaa tggattcggg gaagaagtcg tttgtatggg
    22741 aaaaggacta gcttttcaaa aaaagattgg ggacgagatt tcgccagaag cggtccaaaa
    22801 agaatttgtt ttaagagatt cttttgctaa aaaccaattc cagcagctga tggctgatat
    22861 tccggcagag gagatcgaat gggtcaaaca agtagtcgaa ctggctgaag ataaactaaa
    22921 agttgaattt tcgcctaata tttatctgac attgaccgat catcttcatt atgcgatcac
    22981 acgagcaaaa gaaaatatcc aactgcctaa tccattatta tttgaaacaa aaaaatttta
    23041 tccgaaagaa tatcaagcag cgaaagaggc tcttttgttc gtgaaacaaa agacgggtgt
    23101 ggaactttct gaggatgaag ccggttttat tgcctttcat tttgtgaata gccaacaagg
    23161 aaacgaagac atgcaagtga cgatgaccgc taccacaatg gtaagagata tactgagtat
    23221 catcagtaaa ttcttcggca tcgtgttcga tgaagaatct ttgaattatc agcgtatcat
    23281 tactcatctg cagttttttg cccagcgtta tctcaaaggg gaaggatctg acgagcaaga
    23341 tgaatttctc tacgcattag ttcaaggaaa gtatccaaaa gcgtttcatt gtgtcgaacg
    23401 gatcaatgag tatcttcgac aaacgcaaaa acaggaaatg gggatcgcag agcagattta
    23461 tttaacgatt catattcaaa gagtagtaag tgagaaaaaa agcatccaat cataaggatt
    23521 gttactgtat ggcaggcaaa acctggattg aaaaaatagg ttcacgaatg tttgtgactg
    23581 tttttttcaa tctgggtttt tattttttaa ataaaaaaga aagaaggaaa aaacaatgga
    23641 taatcaagca gttggtcgtc gtgtatggga agcggtagga ggacaaaaga atgtcaaaag
    23701 tttagtacat tgtgctacgc ggttgcgatt cagattaaaa gatgaatctt tagcagatac
    23761 gcaaaagtta aaagaagacc cggacgtgat ccaagtcgta caaagtggcg gtcagtatca
    23821 agtagtgatt ggcagtaatg tagcagatgt ttatcaatcg atcgtggatg aggaaggtct
    23881 tgcagtttcg gaggatggaa aagaagagtc caaaaatatt ttgaatcgtc tgatcgatat
    23941 tatctctagt atatttacac cttttctagg ggcgatggca gccgccgggg tattgaaagg
    24001 attcttatca ttggcaacgg tgatgggctg gctgacagct gatagcggta cttatcaaat
    24061 attgtttgct gcagcagatg gtgtctttac cttcttacct gtcatgcttg cttttacagc
    24121 agctaaaaag ttcaaagcca accagttttt agcagtagca attgctatgg cacttgtgta
    24181 tccagctatc acagcagttg ctggtgcggg agaagctatc agtttctttg ggatcccagt
    24241 gattctttca ccatctggtt atacttcttc tgtcatacca atcattctag ccgtatgggt
    24301 tcaaagtaaa ttagagccat tcgtaaaaaa agtgattccg caatttctgc aaatgatttt
    24361 agttccgctt gtcgttttag tagtgatggt tccattaaca tttcttgctt taggaccaat
    24421 cgggacggtt gccggtaatg ctttaggcgg tctcttcaac tcgatttatg gatttagtcc
    24481 aatcgtagca ggtctaatca tgggaagttt atggcaagta ttcgtgatgt tcggcatgca
    24541 ttggggattc gtaccgatca tgtttttgaa tatcgaacaa tatgggtttg atgtattgat
    24601 gccaatgctt cttccagcca tcttggcaca aggtggggct gctttagctg tagcattgcg
    24661 aacaaaagat acgaaacttc gagcattagg gatttcttct acagtgactt cgttatttgg
    24721 aatcactgaa ccaactgtgt atggtgtgac attacctttg aagaaaccat tcattgccgc
    24781 ttgtatttct ggaggtattg gtggagctat tatcggattt tccggagtaa aagctttctc
    24841 cagcagtttg gtcagcttat tgacgattcc gactttcatt aatacggtgg atggtgtaga
    24901 gtcaaatgtg acagtagcag taatagctac aggaattgct tttgttcttg cattcgtagg
    24961 tacattgatt ttaggatttg acgaacaaac acaagacaat caattagaaa acaaacatgc
    25021 gaatgcagga aaaccgatta cttcagcacg gcatacctta aaaagtcctt taacgggtaa
    25081 agtactgcca ttgagtgaag taccagacca agttttttct tctggcgtga tgggaaaagg
    25141 tatcgctatt gatccagaag taggagaact agttgcacct gcagacggag agatcacgac
    25201 tatttttcca actggacatg ctgtagggat cacaacgact gacggagcgg agattttgat
    25261 tcatatcggg atggatacag tagaattaaa tgggaatggc tttgagatct tagtcaaaca
    25321 aggggatctc gtcaaagcag gagatttatt gattcgtttt gacatcgaag caattagagc
    25381 agctggctat agcgtaatca ctccagttgt cattacgaat actgatgcat ttgccgatat
    25441 acttgaatta gaccaaaaag agattattgc aaacgaagat gttctggcaa ttgtaaaata
    25501 aggaggaaat caggatgcaa tcacgttttt cagaaaaatt tttatggggt ggagcaactg
    25561 ctgccaaatc aattagaagg ggcttatgat cgagacggaa aaggactatc cgttgcagat
    25621 gctatgcctg gaggtaagca gcgctttgcg attatcggca gtgaagaatt tgactggacg
    25681 attgatcaag aaaaatatat ttacccgaat catcgaggaa tcgatcatta cgatcgtttc
    25741 aaagaagatc ttgccttgtt tgcggaaatg ggattcaaat gttatcgttt ttcgattgcc
    25801 tggacacgaa tttttccaaa tggagacgaa ggtactccta acgaagcggg attagaattt
    25861 tatgatcagc tgattgatga gtgcttaaaa tatgatattg aacctgtgat cacgatttct
    25921 cattacgaaa tgcctcttca tttggcaaaa gaatacggtg gctggaaaaa tcgaaaactg
    25981 atcgacttct atgaacgatt tgctcagaca gtattagaga gatacagcag taaagtcaaa
    26041 tattggatga ctttcaatga aatcaattcg gcctttcact ttccagcttt aagtcaagga
    26101 ttagtaaaaa gcaatggagc aggagagtac caaaatattt tccaagcatg gcataatcaa
    26161 ttcgttgcaa gtagcaaagc cgtaaaaatc ggccacgaat tgcgttcgga tatccaaatc
    26221 ggctgcatga ttatctatgc aacaacttac agcatcgatg ccaatccagt caatcaggca
    26281 gcaacaatga tccaaaacca agaattcaat tttttctgca cggatgtcca agtacgtgga
    26341 gaataccctg catacacagc gcgtacccat aaaaaatatg gagtcgatcc ggaaaaatta
    26401 gagcaaacgg aaaaagactt ccagttgcta aaagaatatc cagtggacta catcggattt
    26461 agttattata tgtctacggc aatcaatgag actgatccac aagcagcaac ttcagaaggg
    26521 aacttgctgg gaggagtaaa aaatccgttt cttgaggcaa gtgaatgggg ctggcagatc
    26581 gatccagaag ggctgagaat cgcattgaat gaattataca atcgttacca gaaaccatta
    26641 ttcattgttg aaaacggtct tggagccatt gatcatatgg aagaggatgg cacgattatt
    26701 gatgattatc ggatcgatta tttacgtcgt catatcgaag cgatggctaa tgcagtagca
    26761 gacggtgtag atttgatggg ctatacgcct tggggatgta tcgatcttgt cagtgcatca
    26821 acaggagaaa tgagtaaacg ttacggcttt atctatgtgg atctagacga tgaaggaaat
    26881 gggacactga atcgatcaaa gaaaaaatct tttgattggt ataaagaagt aattgcctca
    26941 aatggcgtaa acttgtaatt aaagcagctg aatgaaaaag aacacacttc tcacatgtga
    27001 tttgtgttct ttttttattt tttatgtgct attcttttag taatagtaaa gcaaagggtg
    27061 acgttatgaa aaggacacgt gtactatact tggaagtcgc agataaaatt aaagaagata
    27121 ttttctcagg aaaatatcca gtcggttcga tgttgccgac agaaagtgaa ttagaagaac
    27181 tgtttcaagt cagcaaaatc actgtccgaa aggcaatcga actgctggca tcagatgaat
    27241 atgtcgcaaa gaaaagtggt cgtgggacga cggttctaag caatcgtcct tacaatcgat
    27301 tatccaaagc ggcaagcttt acgcaaatct tggagaactc ccgatatgaa gtccggaaag
    27361 aaacgctgga tttgcaattg gtccatataa agaaggaaga tcctttgttt tcctttttcg
    27421 gtgagcaagc tgtttgtttt aagcgcttat attatttgaa caatcagcct tatatttatt
    27481 ttgttcatta tcttccacaa gaaatgatgc aattcagcag agaagaattt ggcaaagaat
    27541 ctttgtatcg tttgctgacg aaaaaaggat atactattga ctcattttct gatgatttct
    27601 cggcagtatt tttaacagaa gaagaacaag gaattctgaa aacagacgca cagatcggca
    27661 tcaaacggat acggaaatcg tctacgcaaa ttggggaaat gattgaatat tcggaggcgg
    27721 tctaccatac ggagctgcat ccttaccata ttgattatga aacataaata aaa
//
LOCUS       DACEEZ010000026        27534 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20313_2017
            SAMN12639966-rid9879223.denovo.026, whole genome shotgun sequence.
ACCESSION   DACEEZ010000026 DACEEZ010000000
VERSION     DACEEZ010000026.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639966
            Sequence Read Archive: SRR10023158
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 27534)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27534)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741683.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741683.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:44:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,016
            CDSs (total)                      :: 2,962
            Genes (coding)                    :: 2,889
            CDSs (with protein)               :: 2,889
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 36 of 73
            Pseudo Genes (incomplete)         :: 29 of 73
            Pseudo Genes (internal stop)      :: 18 of 73
            Pseudo Genes (multiple problems)  :: 8 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27534
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639966-rid9879223.denovo.026"
                     /isolate="E20313_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(26..466)
                     /locus_tag="IWO89_001214"
     CDS             complement(26..466)
                     /locus_tag="IWO89_001214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spx/MgsR family RNA polymerase-binding
                     regulatory protein"
                     /protein_id="HAQ0455984.1"
                     /translation="MMIKLYTGSGCSSSRRAKRWLIVHDIPFIEQNLIRQPLTKNEFI
                     HLLKLTNNGVTEMLSTHSISYKNLVHRIDELSLSELYIEIRTSPSLLKKPIIYDQSAK
                     LLTGFSEDKIRMFLSKTKRKQELNELLLSGHLVDFKENNLFAMS"
     gene            626..1132
                     /locus_tag="IWO89_001215"
     CDS             626..1132
                     /locus_tag="IWO89_001215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HAQ0455985.1"
                     /translation="MSNTADAIVVIDLQKGVCFDSSPIHELETVTALVNKRIKTYETI
                     GKPVIFVQHEDAQLVPQTKPWELLSELSIPEHSYFVRKTHANSFYKTNLSVLLTELDC
                     HSIELCGAQAEYCIDTTVKFAHGLGYQLMMCRGATTTYDNPFMKAKETIAFYEKIWDK
                     RFLTFLEQ"
     gene            1300..3213
                     /locus_tag="IWO89_001216"
     CDS             1300..3213
                     /locus_tag="IWO89_001216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HAQ0455986.1"
                     /translation="MNSLRTKQVLKGALVILFAVSTSVPQWTVYGESISSESESREVE
                     TTETTDAGNETKSSTSQTETNTETTVETSVTSTDQETTATTDNQQTEEATATKMSNDE
                     SSSAKVSDKENGTENDDIGKGENFNGKEYLNYFGKQMTEILNGPTEDIKSKLIELLVT
                     ELAVNDYGVDKEIIKHLIATYLLYDIAFAKNSVLMSMLGGTDDGTTPTENTGFYPNTK
                     QLHQTVLNKQDGKMMDVYAYYVDQGSDKTVMIHGGFRGNWNNGIVTEEYNDFYKAGYN
                     LLFVDSRATGNSGGDYVTYGQYESDDVLYWINQEVRERPSQKILLYGGSMGAATMMSV
                     LAKDIPVNVKGIIENCGFASIDEQLRFTYSQTVAPALPDAIKNQLDIIGDQEHEDLFM
                     GLLKQYYFDQEMHLDTKAALPTIGMSDSLPKLIIHGTADDVVPVSNAQKLYELSGGYK
                     DLLLVEGAGHGKAQEVDHAAYTKHVTDFLKVVFHDQINVKYVDENNKSLLKDQDEIQL
                     YGAYGENYMTEQKTFEGYELANVEGPTEGIFNETIPTIIFYYKKIPVAPPKKQDPTEN
                     ADNKGKNEPREDQSSDDTQPIQMTVKVQKKTDAKKRMLPKTGEKKHSLLIYSGGFLSV
                     STIITWIWRRKRG"
     gene            3218..4219
                     /locus_tag="IWO89_001217"
     CDS             3218..4219
                     /locus_tag="IWO89_001217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HAQ0455987.1"
                     /translation="MKWFKRIVIAVPVIIVVILVTVFLINQITPKPISLLVRKQFDTS
                     EKEQVYARPDDFQQKIEQIDIKKEISYPSKYKNNNMDIYTPKVEKKKLPILFWMHGGA
                     YVGGDKNDCRDYLEYLCSDTQQIIVNIDYERSPEAKHPTPVIQLNEAIQAAKKEIKDQ
                     ADWSNISIGGNSAGAQISGEYLLALQNEEIKKADHLDPTLENKQITKFISLSGLLDPS
                     ALTQVSDKISSFLYEKCGWAYFDNKDFEQSEKVKSLALVRHADRWTQNTFLTDGNTNT
                     FTKQMEEVTSVFEKAGVKVTKVDYAEKNAVLNHEYQFDFSNKQAQETYQKLVMFFKE"
     gene            complement(4421..4627)
                     /locus_tag="IWO89_001218"
     CDS             complement(4421..4627)
                     /locus_tag="IWO89_001218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3955 domain-containing protein"
                     /protein_id="HAQ0455988.1"
                     /translation="MKAIHISLLFFASALILFAIHLCSQPYINNNGILVEPAFFCLPL
                     GFLSLLASAGFGFVAFFKRSKQQI"
     gene            complement(5273..6121)
                     /locus_tag="IWO89_001219"
     CDS             complement(5273..6121)
                     /locus_tag="IWO89_001219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HAQ0455989.1"
                     /translation="MSVSIRLQIKTMYNDFSPKEQAIADYILENPSKVSHSPISNLAS
                     ELGIADSTFFQFTKKLGYNGFKDFKMAMLIQENDLSAISIHENIQKTDTELTMAQKVF
                     ESSISTLNDTKKLLQQKDLKKAATIINESKRVYFFGVGGSEIVATDAYHKFLRSPIAT
                     SHSTDYHIQLMEASLLTEKDCAVLISHTGQSKETIHIAETVRKTGAKTIVITSQANSS
                     LAKLGDVVFISISEETEFRSEALASRISQLSILDSLYVILMFYNEKESRRSIAKVRHT
                     ISEIKE"
     gene            6285..7187
                     /gene="gnd"
                     /locus_tag="IWO89_001220"
     CDS             6285..7187
                     /gene="gnd"
                     /locus_tag="IWO89_001220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="decarboxylating 6-phosphogluconate
                     dehydrogenase"
                     /protein_id="HAQ0455990.1"
                     /translation="MEIGVIGLGKMGLNLALNLKEHHHKVQGLDISSTAVSQARENDI
                     PAYQEISEFLATFEQKKIIWLMLPAGEITENMLRKLFPKLGPGDIIIEGGNSYYKDSI
                     RRAEEFQKNEIGYLDCGTSGGIEGARHQACLMIGGDKETFLSVEQLFKDVAIERGYLY
                     AGASGSGHFLKMIHNGIEYGMMQAIGEGFQLVQKSEYAFDLEQVARVWNHGSVIRGWL
                     MEIMEDQLNEHPNLSNYRGIVSASGEAKWTVETALEMAVPVPVIATSLFMRNLSQEED
                     SFSAKVVSALRNGFGGHEIVEKGE"
     gene            7189..8190
                     /locus_tag="IWO89_001221"
     CDS             7189..8190
                     /locus_tag="IWO89_001221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyacid dehydrogenase"
                     /protein_id="HAQ0455991.1"
                     /translation="MPDYHIAIVNSSSFGRVFPHHLERLKSVGDVHFFTVDQEIPGKE
                     LAELLKGFNIIIASVTPFFRRDFFENKDELLLISRHGIGYNNIDLKAAKEHQTIVSII
                     PALVERNAVAENNITNLLSMLRRTTQAQARVKDDRWEDRAEFVGRTLFNKTVGVIGAG
                     NTGSGVIEILRNGFRCDVLAYDPYKSDLYIESFGAKKVELQELLEQSDIICLCANLTE
                     ENYHMISDKEVDQMKENVYLSNSARGALVDEEAMINGLKSGKIAGYATDVLEEEPGRQ
                     DHPYLAFEQVVITPHISAYTRECLEAMGEKCVRDVEQISQHLLPERSVQTKSNYIKG"
     gene            8187..8471
                     /locus_tag="IWO89_001222"
     CDS             8187..8471
                     /locus_tag="IWO89_001222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase family
                     protein"
                     /protein_id="HAQ0455992.1"
                     /translation="MMKKMVNGLKVKTGPQFYLYEEGGISKVSDLLKSYGAKRVLVTH
                     GTVSWEKALPKLVFLNDETIQFFYHRYSGECSYAEARRIATIIKKMKSIS"
     gene            8504..9301
                     /locus_tag="IWO89_001223"
     CDS             8504..9301
                     /locus_tag="IWO89_001223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase family
                     protein"
                     /protein_id="HAQ0455993.1"
                     /translation="MGYSANLARIDFGVIPTLASNCAPWTPLAVMYKENGQAEGKTEH
                     FYRQAAFFITDPQLVIDSPVPYFIAGLADTLAKWYESELILEQDFLQDESFLKLAQDT
                     AKICKEEILNHSAKAIQDMAQRKLTSEFKQLSEIIFAVAGLVGGLGDKYARNVAAHAM
                     HDAISKYIPESHRFLHGEKVAYGIFYQLALEGRWEKIDELYPLYRELELPVSLEQMGL
                     FPKDKQIIDKMAEFIDSKEKVHLIPIEVTQKSLIQSMMELEGYIRKI"
     gene            9315..10148
                     /locus_tag="IWO89_001224"
     CDS             9315..10148
                     /locus_tag="IWO89_001224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016184786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAQ0455994.1"
                     /translation="METLSVVQSLLIALWVAAVMSRWLGGGATLTLRFSPLMTGLVVG
                     LIMGEVAQAMIVTAALQMIYMGVFSPGGSMPAEPSIAAAIAVPVALLGDLSPEAAIAV
                     AVPVGLLGSYLYQFRFFINTFLGKYTDKAVEDLNTKAMARSIILYPTVASFLLFVPLV
                     FIALYLGAPLIADVIKALEGTVVIHILEVVGGGLAAIGIATTVYVIGRKDYLVFFFLA
                     YFMSVVLKSLDITMVTYAIFGIIIALIFVQSQKGKQVQPATSNNNSASFDDEDDYDDG
                     F"
     gene            10166..11023
                     /locus_tag="IWO89_001225"
     CDS             10166..11023
                     /locus_tag="IWO89_001225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HAQ0455995.1"
                     /translation="MAEIMDNQTGAPEEITKKDVTKAYLRWHFANEIPHSFERYLAPS
                     LLYGMMPILKKLYKDEDSLKAAYKRQLLFFNTQLSWGGGVITGLMSSMEQQRAEEEYE
                     EKEILMQDDLMYNTKAGLMGALAGIGDAIDSGTVQYIFIAIAVPWAQQGSPLGALFPF
                     ICFGLYQVLLGVFFARQAFTMGRNATGLKQNTGVQTAIEMLSVLGLFMMGVLAGNYVK
                     VESSLKFAISGREFVIQEILDQIVPGILPLAVVMGVYLFYTKKGLKVTQALLWLTGIL
                     IVLAGIGIL"
     gene            11045..11554
                     /locus_tag="IWO89_001226"
     CDS             11045..11554
                     /locus_tag="IWO89_001226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAQ0455996.1"
                     /translation="MGVVNLARVDERLIHGQVMVTLSQKSGVNSIFVVDEVVAKDKFM
                     RDLYKNAGSRTGQKTIVITPEKAKFYWDEYQFKEYNCILIAKTVQTIYDLVKHGIPME
                     ELNIGGIAQKNPDTDLLVTKSVYLNKSDAEKLKEMNEQYGVKNIYFQATPSAPKTGLK
                     DVLNKFDLS"
     gene            11591..11992
                     /locus_tag="IWO89_001227"
     CDS             11591..11992
                     /locus_tag="IWO89_001227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0455997.1"
                     /translation="MKKNELFRDWEFRYRYIYRKRRTKKSKQRFLSALVSDIYSMRTD
                     VTVIAYDTLAYRSKNIYVGDIEKAEKVICTYYDTPVHALGSYFMFDWKDQRKKTIYSI
                     LLSFILLFSLGWWGMMIYNKNPHHVFDLLSV"
     gene            12104..12457
                     /locus_tag="IWO89_001228"
     CDS             12104..12457
                     /locus_tag="IWO89_001228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0455998.1"
                     /translation="MLEMIRTIDDPSVAYAFVDEGCYGEKGLDSVRSGMKKEAILFYL
                     DSVGADTPLQFSGNYFSNKEQWLKQVDKLKEKNVNYIFSARKKQAQFFYLTKTDLRGK
                     TFNWQNANQIIALFR"
     gene            12491..12934
                     /locus_tag="IWO89_001229"
     CDS             12491..12934
                     /locus_tag="IWO89_001229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIA"
                     /protein_id="HAQ0455999.1"
                     /translation="MLEIMIVTHGKLSEGLKDAAEVIVGTSSHIETMCLESGEDIQTF
                     GEKVKEKLTMINTAEPMLVFVDLISASPYNQALLAVNELTEEKKAATYVIGGVNLPML
                     LEAINHQMLHTPIEEAVEQIVKQGKDNLTVWHASAEEKKLDEDDF"
     gene            12951..14036
                     /gene="uxuA"
                     /locus_tag="IWO89_001230"
     CDS             12951..14036
                     /gene="uxuA"
                     /locus_tag="IWO89_001230"
                     /EC_number="4.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannonate dehydratase"
                     /protein_id="HAQ0456000.1"
                     /translation="MKWGFRWYGEKGDSIPLKYVRQIPGINGIIGTLLTKMPGDVWEI
                     SEIEELKASVESQGLELLGIESVAIHDAIKAGTDERDYYIDNYIRTIKNLSRCGVRLI
                     CYSFKPIFGWAKTDLFNENNDGSYSLLYDQKIVDQMEPGEMYQLIHSQSKGFKLPGWE
                     EERLKKFAQLEKLYEGMTEEELFENLTYFLEKVIPVCEKADVKMAIHPDDPPWELFGF
                     PRVTKNLGDLKKILNAVDSPYNGLTLCTGSLGANPENDLVEIIHEVGSRIHFVHFRNV
                     EFLGERKFKETAHPSTEGSLDMYHIMKALVQEGFDGPIRPDHGRTVWGEVAMPGYGLY
                     DRAMGLTYMQGLYEAISKEKPLGEHYE"
     gene            14029..14742
                     /locus_tag="IWO89_001231"
     CDS             14029..14742
                     /locus_tag="IWO89_001231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HAQ0456001.1"
                     /translation="MNKIKLFIFDMDGLLLETGRLAYRAYVKSAQKYDYEMRKEVYYL
                     LTGQTEMAIREQMGILYGEDVPYIKWREAINQYKEKIVKEDKRVYTKKGAEEILSFAK
                     ERGIHTIVASSNTRVKVEMYLKMENLYALFDQIISGDDVKKGKPEPEIFLKACSKMNI
                     PPSEALVFEDSIAGIEAARRAGILSFLIKDHLIGLPDHNGKHKLKVDVSLYESNKADY
                     EFYDLHQAKRFLESKGLYL"
     gene            15494..15694
                     /locus_tag="IWO89_001232"
     CDS             15494..15694
                     /locus_tag="IWO89_001232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HAQ0456002.1"
                     /translation="MNNGTVKWFNADKGFGFITGEDGNDVFAHFSAIQGDGFKTLEEG
                     QAVSYDVEDGQRGPQATNIVKA"
     gene            16262..16825
                     /locus_tag="IWO89_001233"
     CDS             16262..16825
                     /locus_tag="IWO89_001233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0456003.1"
                     /translation="MLYIKKGIIVISFFKEYLEIINFRLKTKKESVIILVYFQGGNLM
                     IKKLFLLSILTLAMAGCSDSGLATELITKSTEATTEELKCGNDHSDYRGLNRRCMGIL
                     GRSNCKHRCSNRRRCRRSNRKIKRKIKRSNLCCASYTDVEDAVLLNHVRRCSFMSREV
                     GDEPKLEYAFSFAREDEDNPKTYDILA"
     gene            complement(16886..17251)
                     /locus_tag="IWO89_001234"
     CDS             complement(16886..17251)
                     /locus_tag="IWO89_001234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0456004.1"
                     /translation="MRLWHEKLISKLPRQQLLGQHRECCALRGNGWGKKHATVDYVFH
                     YSPYRLFQYHQLVMDEMEKRGYSPAPEWKNPMYRGKSCEAFFDLPTVERNVPIYPEHD
                     EVYLAECLANLEQKGIYLT"
     gene            complement(17327..17572)
                     /locus_tag="IWO89_001235"
     CDS             complement(17327..17572)
                     /locus_tag="IWO89_001235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAQ0456005.1"
                     /translation="MSIEEAILLDTLRCRLNFLRTGRSLSQVAVSSALSIPLFKYRKY
                     EEGEEIPSIIELIYIADFYDISVDYLIGRSETASKLK"
     gene            complement(17642..17896)
                     /locus_tag="IWO89_001236"
     CDS             complement(17642..17896)
                     /locus_tag="IWO89_001236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0456006.1"
                     /translation="MITLVRVLFWVPAVALVASIVYLMNWNKERFYLAILTLPAIYFM
                     WKVFNYNYFEPDSVFIEELSGLVLSLLIVILYLIRLNKKH"
     gene            complement(18060..18383)
                     /locus_tag="IWO89_001237"
     CDS             complement(18060..18383)
                     /locus_tag="IWO89_001237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0456007.1"
                     /translation="MLGVIILPFIAILFDLVAAAAYFLQLNYQTSGILFIGMIFQGVI
                     TLLLLVMMLSYKGKRYARIQTKVFVKYVSIRYGIIILSFLVNAIVLFLYVLNYLDINP
                     LIFSR"
     gene            18570..18643
                     /locus_tag="IWO89_001238"
     tRNA            18570..18643
                     /locus_tag="IWO89_001238"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:18604..18606,aa:Ile,seq:cat)
     gene            complement(19028..19525)
                     /locus_tag="IWO89_001239"
     CDS             complement(19028..19525)
                     /locus_tag="IWO89_001239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HAQ0456008.1"
                     /translation="MLVIIDMQNHILDPNSEFYIPESDQLAERIAQRLAKARQNNEYI
                     LFTRDIPVELKDKEEEREDLQLIPLLSPQENERVIKKYYFTIPPETLAEIQETLFESK
                     EEQKEIEVVGIETNLCVLSNLIGLQSAFPEADFFVDPMLVSSRKHGESALELLKDFNV
                     SIVES"
     gene            complement(19565..20374)
                     /locus_tag="IWO89_001240"
     CDS             complement(19565..20374)
                     /locus_tag="IWO89_001240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HAQ0456009.1"
                     /translation="MKLAAIDLDGTLLDRNGKIPEENIQALRKFDENGGIVTVATGRN
                     SISAKDIFSELGIGGYLISSNGSLIAEMKDGKMAKVLRRSKIDSDTLRKAFFYAQEAK
                     ISIIASRETQDDQLTFDESNLVKDDPYYEHFNLQNHSFDQILNMLEDPSLSYLKLALT
                     DKSEEKLQKLQRDLQADGIQSVFSDPHFLEITPTGITKAHSLVFLADYLGIESSDIMA
                     FGDQENDIAMLNYSGLSIAMGNAQRQVKEIANQVTLTNEEAGVAYILKNFY"
     gene            20708..21565
                     /locus_tag="IWO89_001241"
     CDS             20708..21565
                     /locus_tag="IWO89_001241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 8 protein"
                     /protein_id="HAQ0456010.1"
                     /translation="MEKRYGVVPVVTASDENYAPYLSVMIATALENCNKTRRIKFYVI
                     DDGLSEYSKEGLEETVNKYSSNASIQFLTVEKDIYEDFLVSDHITTTAYLRISLPNLL
                     AKEDYKKVLYLDSDVLVLDDIVKLYDEPLNGKTIGAIIDPGQVKALERLGIDSDDLYF
                     NSGVMVIDIDQWNKKEITEKTIHYLSENGDRIIYHDQDALNAVLYEDWEQLHPKWNMQ
                     TSLIFERHPAPNEKYERQYKEGNEKPSIVHFTGHDKPWNTLKDHPYTNLYLKKLAHST
                     LTKVGEINE"
     gene            21558..22460
                     /locus_tag="IWO89_001242"
     CDS             21558..22460
                     /locus_tag="IWO89_001242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 8 protein"
                     /protein_id="HAQ0456011.1"
                     /translation="MNKKEIAVVASCNTKFVPHLAALFVSVLDNCNPSKFVRFYVIDD
                     DIDFESKQLLRFSVKNARMNSDVEFLKINKEFFTNVVISDRIPETAYYRIAIPELFRG
                     TEVERILYMDCDMIALQDISKLWRLDFGDSIVAAVEDAGFHQRLEKMKISAKSMRYFN
                     SGLMLINVKKWLDENITQKVLDFIEHNPEKLRFHDQDALNAILHDRWLPLHPRWNAQG
                     YIMAKAKKHPTAAGEREYEETRNNPYIIHFSGHVKPWSKDFEGPTKKYYEKYAGMTAF
                     RCVAKFPKYPKYAKVQPKTDVVVK"
     gene            22493..22843
                     /locus_tag="IWO89_001243"
     CDS             22493..22843
                     /locus_tag="IWO89_001243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster biosynthesis family protein"
                     /protein_id="HAQ0456012.1"
                     /translation="MEITMSKNAVETLIEKVGENTKIALALINDSDPFLRDKGAFAKG
                     SFFQIIPFVSEFGEYATKIEHPLLDIYTSKLEQNYFGKRLNMDFNKQLDSFSLENEIA
                     VLDYNIKLKNCFFS"
     gene            22877..23814
                     /locus_tag="IWO89_001244"
                     /pseudo
     CDS             22877..23814
                     /locus_tag="IWO89_001244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291265.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
     gene            23892..24785
                     /locus_tag="IWO89_001245"
     CDS             23892..24785
                     /locus_tag="IWO89_001245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HAQ0456013.1"
                     /translation="MTEPKLIDPRNLYHTEKFPKQDQDTPALQQNMIPVPDCGEESYE
                     GNNQLENRRVLITGGDSGIGRAAAIAFAREGADIALQFFPGEEKDAEEVAKYVKVAGR
                     KIVLLPFDLRDEQAPKEIIDKAVSELGGLDTMVLNAGQQISCQSIAELPLEQVKDTFM
                     INIIAMFALVKEAVPHIPAGGAIVTTTSVQAFNPSEHLLDYAATKASIANFTIGLAKQ
                     LAPKGIRVNGVAPGPIWTPLQLDEGQPSDSIPEFGQQSLLERAGQPGELAPVYVFLAS
                     NKASYVTAQIYGVTGGEAINL"
     gene            24823..25386
                     /gene="amaP"
                     /locus_tag="IWO89_001246"
     CDS             24823..25386
                     /gene="amaP"
                     /locus_tag="IWO89_001246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline shock response membrane anchor protein
                     AmaP"
                     /protein_id="HAQ0456014.1"
                     /translation="MNKGVKTIGVIGTLLLLSVLLFAILLSATYPLPFQLEKFRFFSI
                     TNSYVQQYVFWLAVAFAILTIITLLVFLFYPKTIGNFLLKHGDGELTLDKKAIEGMVR
                     SHLHEDEFIHSPKVNVKATKNKIAVNVKGELKRTSALIGKTGTLMKEIENEVQHILGT
                     KEPVKVSVKYTGFEEPAKRSNDRKRVE"
     gene            25400..25588
                     /locus_tag="IWO89_001247"
     CDS             25400..25588
                     /locus_tag="IWO89_001247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2273 domain-containing protein"
                     /protein_id="HAQ0456015.1"
                     /translation="MKELFSTYKLPIIFGVLGLILAVLWITVGFFKTLLILIMAAIGV
                     AIGFYLKQTKILDHYFKS"
     gene            25601..26503
                     /locus_tag="IWO89_001248"
     CDS             25601..26503
                     /locus_tag="IWO89_001248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HAQ0456016.1"
                     /translation="MNNNNKNNNNKMNNQKPVSAHPDPTTPNQSNGVNDEKNKTAPVS
                     AHPDPVVPNVQQGVEEKKHDAEKHAPHHPGTPESKVPEQHDAIDPSKPVEEQKHPSDH
                     AKAQEKLSSDHTNGQGKLGSDHTNNQGKHDSEQEDSMRGELNYEDKVVQKIIGIAIEQ
                     VDGLLSANGGFFSNVAGKLVNTDNVTAGIETEVGKKQVAVDMDVIVEYGKDIEKIFEE
                     MQEVIGKEVKNMTHLEVIEVNANVVDIKTKEEFEKDQETVQDKVTDAAKKTGEFASNQ
                     TDKVKSAAGSGAQKVKENTEPRVQ"
     gene            26535..26777
                     /locus_tag="IWO89_001249"
     CDS             26535..26777
                     /locus_tag="IWO89_001249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HAQ0456017.1"
                     /translation="MGFIWSLIVGGIIGAIAGAITNKGSSMGIIANVVAGLVGSALGQ
                     ALLGSWGPSIAGMAIVPSLIGAIILVLIVSFFARKL"
     gene            complement(27033..27404)
                     /locus_tag="IWO89_001250"
     CDS             complement(27033..27404)
                     /locus_tag="IWO89_001250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0456018.1"
                     /translation="MPFIVYFFLSLLVIYIPLPTFFLLVNRLAEQHDTIAALTKLCLL
                     LFVCLDYFTSYYWLKQTRVKKRYYFFLVFFNLFEFLIHCFLLHQYHTIHLWIIWVFKV
                     VVLFCMFTFPISRKFREALFY"
BASE COUNT         9628 a         4447 c         5559 g         7900 t
ORIGIN      
        1 gaaaagcaga ttgtttatgc caaaattaag acattgcaaa aagattattt tctttaaaat
       61 cgacaagatg accagaaagc aagagttcgt tgagctcttg ctttctttta gttttagaaa
      121 ggaacatcct gattttatct tcgctaaatc cagtcaaaag ttttgcagat tggtcgtaga
      181 taatcggttt tttcaataaa gaaggggatg tacgaatctc aatatatagt tcactcaaac
      241 tgagttcatc tattctgtga acaagatttt tataactaat agaatgagta gagagcattt
      301 cggtcacgcc gttgtttgtc aattttagta agtggataaa ttcatttttg gttagtggtt
      361 gacgaattaa gttctgttcg ataaacggta tatcatggac gataagccaa cgttttgcgc
      421 gtcttgaaga cgagcagcca gaacctgtat acaatttaat catcatacat tcctccaata
      481 gctaagtatt tatgaaataa taacatgaag aaatagtcga aaagtgccat ttagaggtgt
      541 aaaataagaa aatgttgtca cgtataatgg aactagctag ataagaaata ctattaaatc
      601 aagaaaaaag aaagaaggca caagtatgtc aaacacagcc gacgcaatag tagtgatcga
      661 tttacaaaaa ggggtatgtt ttgattcttc gccgatacac gaattagaaa cagtgacagc
      721 attagtgaat aaaagaatca aaacatacga aacgattggg aaaccagtta ttttcgttca
      781 acacgaagat gctcaattag tgccacaaac aaaaccatgg gagctattgt cggaattatc
      841 tattcctgag cacagttatt ttgtgagaaa aacccatgcg aattcatttt ataagactaa
      901 tttgagtgtt ttattaactg aattggactg tcattcgatc gaattatgtg gggcacaagc
      961 agagtattgt atagatacga ctgtgaagtt tgctcatgga ttaggatacc agcttatgat
     1021 gtgtagagga gcaacgacaa cttatgataa tccttttatg aaagcaaagg aaacaatcgc
     1081 tttttacgag aaaatttggg ataaacgatt tctgacattt ttggagcaat aagttgtttc
     1141 ttcgtttaaa aaaagcagaa tggatactgg tttatccatt ctgctttatt acgtaaaatg
     1201 agaaaataat cctgttctat ttttatggga taaaccattt attttttgta taaaaaatgt
     1261 ttgcgtttac atataattct cctagaaaca taaaaaggag tgaatagctt gagaacaaaa
     1321 caagtattaa agggagcgtt ggtcatctta tttgctgtaa gtacaagcgt cccgcaatgg
     1381 acagtatatg gagagagcat ttcttccgaa tcggaatctc gggaggtaga aaccacagaa
     1441 actactgatg ctggaaatga aacgaaatcg agtacttcac aaacagaaac aaatactgaa
     1501 acaacggttg agacatctgt tactagcaca gatcaagaaa cgaccgctac cacagataac
     1561 caacaaacag aagaagcaac agctacaaaa atgtcaaacg acgaatcctc ttctgctaaa
     1621 gtaagtgaca aagagaatgg gacagaaaat gatgatatcg gtaaaggtga aaatttcaat
     1681 ggtaaagaat atttgaatta cttcggtaaa cagatgacag aaatacttaa tggaccaaca
     1741 gaagacatca agtcaaaatt aatagagtta ttagtcactg agcttgctgt taatgattat
     1801 ggtgtagata aagagattat caaacatctg attgctactt atctattgta cgatatcgct
     1861 tttgcaaaaa acagtgtgtt gatgtcgatg cttggaggta cagatgatgg gacaacgcct
     1921 acagaaaaca caggcttcta tccaaataca aaacaacttc atcaaactgt actaaacaaa
     1981 caagatggca aaatgatgga tgtatatgca tattatgtcg atcaaggttc agataaaaca
     2041 gtcatgatcc atggcggatt tcgagggaat tggaataacg ggatcgttac agaagaatac
     2101 aatgattttt ataaggccgg gtataattta ttatttgttg attccagagc gactggtaat
     2161 agcggaggcg attatgtgac ctatggacaa tatgagtcag atgacgtcct ttactggata
     2221 aatcaagaag ttcgagagcg gccatctcaa aagattttac tttatggtgg ttcgatggga
     2281 gcagctacga tgatgtctgt attagcgaaa gatattcctg tcaatgtcaa aggaattatt
     2341 gaaaattgcg gatttgcatc aattgatgaa caactacgtt ttacgtacag ccagacagta
     2401 gcccccgcac taccggatgc cataaaaaat cagttagaca tcattgggga tcaagagcat
     2461 gaagatttat ttatgggcct tttgaaacaa tactattttg atcaggagat gcatttggat
     2521 acaaaagctg ctctacctac gattggaatg tcagattcat tgcctaaatt gattatccat
     2581 ggaacagcag atgatgtagt acctgtgtct aacgcgcaaa aattatatga gttatcagga
     2641 gggtataaag atcttttatt agtagaagga gcagggcatg gaaaggcaca ggaagttgac
     2701 catgctgcct acacgaaaca tgtaacggac tttttgaaag tagtgttcca tgatcagata
     2761 aacgtaaagt atgtggatga aaacaataaa agtctactca aagaccagga cgaaatccag
     2821 ctttacggag cttatggaga aaattatatg acagagcaaa agacattcga aggatacgaa
     2881 ttagcaaatg ttgaaggacc gactgaaggg atttttaatg agacgatacc aactattatt
     2941 ttctattaca aaaaaatccc agtagctcca ccaaaaaagc aagatccgac agagaatgca
     3001 gacaataagg gaaagaatga acctcgagag gatcaatcaa gtgatgatac gcaaccgatt
     3061 cagatgacag tgaaagtaca aaaaaagaca gatgcaaaaa aaagaatgtt gccgaaaact
     3121 ggagaaaaga aacattcgct tctcatttat tcaggtggat ttttatctgt tagcacgatt
     3181 atcacatgga tttggcgaag aaagagaggg taaatgaatg aaatggttca agcgaatagt
     3241 gattgcagta ccagttatca tcgtagttat tttagtcact gtatttttga ttaatcaaat
     3301 cacaccaaag ccgattagct tgcttgttag aaaacaattt gacacaagtg aaaaagaaca
     3361 agtatatgcc cgaccagatg atttccagca aaaaatcgaa caaatcgata taaaaaaaga
     3421 aatatcctat ccttcaaagt acaaaaataa taatatggat atctacacac cgaaagtgga
     3481 aaagaaaaaa ttaccgattc ttttctggat gcatggaggc gcatatgtag gaggggataa
     3541 aaatgattgt cgtgattatt tggaatatct atgttcagac acgcagcaaa tcatcgtaaa
     3601 tatagattat gaacgatcac cggaagcaaa acatcctaca ccagttatcc aattgaacga
     3661 agccattcaa gcagctaaaa aagagataaa agatcaagca gattggtcga atatttcgat
     3721 tggtggaaat tcggcaggtg cacaaattag tggagaatat ttgttagcac ttcaaaatga
     3781 agaaattaaa aaagctgatc atcttgatcc gactttggag aacaagcaga ttacgaagtt
     3841 tatttctttg tctggtctgt tggatccttc tgcacttact caagtaagtg ataaaatctc
     3901 tagctttctt tatgaaaaat gtggatgggc ttactttgat aataaggact ttgaacaatc
     3961 agagaaggtc aaaagtttag ccttggtcag acatgcggat cgttggacgc aaaacacatt
     4021 cttaacggat ggaaatacaa atacgtttac gaaacaaatg gaagaagtaa cttctgtatt
     4081 tgagaaagcg ggggtgaaag tcacaaaagt agattatgcg gagaaaaatg ctgttttgaa
     4141 ccatgaatat cagtttgatt tcagcaataa acaagcgcaa gaaacatatc aaaagttagt
     4201 aatgtttttc aaggagtaaa gtcttatgat gcaaaagagg ttgtaatctg tcacttagct
     4261 ccaagtcaca aggagcaatt tcaataaaca agcttttcat ggtttatgaa tattgtaact
     4321 tgtgactgag gagttagaga tggcaagggg caccttttat tcgaaatttg ggaatatcac
     4381 acgattattg tcacgatctc ttttacgtaa attttataag tcagatttgt tgttttgatc
     4441 gtttaaaaaa agcaacaaat ccgaagcctg cggaggcaag aagggacaaa aagccgagtg
     4501 gtaagcaaaa gaatgctggt tccactaata ttccgttgtt attgatataa ggctgggaac
     4561 aaagatgaat agcaaataaa atcaatgcac tagcgaaaaa aagcaaagaa atatggatag
     4621 ctttcataag cagcacctcc ttttttataa ttgtatgata aatagattaa gaatctataa
     4681 atgtgacaag tttgttagga tgtgaataaa atgatcatct agcgaacttg aaacaaagat
     4741 ggctaatgat aagcaattaa tgattcagca atcagatagg tacaaagttt accatcatac
     4801 gtctagacca ataatagaaa atatgggaat ataatagaaa agatttagga tacccccaaa
     4861 agaatttccc caaaaattcg atgtatccta aactttttta ttttacataa caatcatatc
     4921 aactgtgaaa taaaattaca aatgataacc taaaatgagg ccaaaagcca gtgttatcaa
     4981 ggaaatactt aattcaccaa aatttttttt aattttttag aaaataagaa acaaaaataa
     5041 taaaaaaaag aaaagataaa ttttttacta ctgtatacta actttgttat cgcatctaac
     5101 aagtaactcg aataatacca atacaacctt ctccaaagat gcgatgacat acgttctaac
     5161 ccaccgacta ttgatgttct gtaccaccct tgcagaacca aaaaagccac taccaaatat
     5221 ttggtagtgg ctttttttgt tttatggatt tttcgctttt tctgttatgg tgctattctt
     5281 tgatttcaga aatagtatgt cgaactttag cgatagaacg acgtgattct ttttcgttgt
     5341 aaaacatcaa aatcacatat agagagtcca aaatgcttag ttgagaaata cgggaagcta
     5401 aagcttcaga tcggaattct gtttcttcag aaatagaaat aaaaacgaca tcgccaagtt
     5461 tagcaaggga agaattagct tggctagtaa taacgattgt ttttgctcca gtttttctta
     5521 cggtttcagc aatatggatt gtttcttttg attgccctgt gtgagaaatc aaaacagcac
     5581 agtctttttc cgtcaataaa gaagcttcca tcaattgaat atggtaatcc gtgctgtgag
     5641 aagttgcgat aggggaacgc aaaaatttat gataagcgtc agttgctaca atctctgaac
     5701 cgccaacacc gaaaaaatag acacgtttag attcattgat aatagtagcc gcttttttta
     5761 gatccttttg ttgtaacaac ttttttgtgt catttaaagt tgaaatacta gactcgaaca
     5821 ccttttgcgc catagtgagc tcagtatctg ttttctgaat attttcgtgg atagaaatag
     5881 cagatagatc attttcttgg atcaacatgg ccattttaaa atctttaaat ccattataac
     5941 caagtttttt tgtaaattga aaaaaagtag agtcagcaat gcccagttca cttgctaaat
     6001 tgctaatagg actatgactt actttgcttg gattttctaa aatataatcg gcgatagcct
     6061 gttcttttgg gctgaagtcg ttgtacattg ttttgatttg tagtcttatg gagacgctca
     6121 tgagaattcc tcattttcat aataagatta tcattttttc cgttctcatt aatgttaaag
     6181 atacaaggag agtaggaaaa aatcaaacaa aaaatatttt tgtaaaatct atttacaaaa
     6241 aaattttttt gtataatgag ggtataagaa atggaggcgc tatcatggaa attggtgtaa
     6301 ttggtttggg aaaaatggga ttgaatttag ctttaaactt aaaagagcac catcacaaag
     6361 tacaaggact cgacatttca agtacagctg ttagtcaggc aagagaaaat gacattcctg
     6421 cttatcaaga gatctctgaa tttctagcta cttttgagca gaaaaaaatc atctggttaa
     6481 tgcttcctgc tggagagata accgaaaaca tgttaagaaa actttttcca aaactcggac
     6541 caggagacat tattattgaa ggcggaaact cttattataa agattcaatc cgtcgagcag
     6601 aagagtttca gaaaaatgaa atcggttact tggattgtgg gacttctgga ggaatcgaag
     6661 gagcacgaca tcaagcctgc ttaatgattg gaggagataa agaaactttt ctatccgtgg
     6721 aacaattatt caaagatgta gcaattgaaa gaggatatct gtatgctggt gcatccggaa
     6781 gcggacactt cttgaaaatg atccataatg gaatcgaata tggaatgatg caagcaatcg
     6841 gtgaaggatt ccaacttgta caaaaaagtg agtatgcttt tgacttggaa caagtcgcac
     6901 gtgtttggaa tcatggttcg gtgattcgcg gctggctgat ggaaatcatg gaagatcagt
     6961 taaacgaaca tcccaattta agtaattatc ggggaatcgt ttctgcatca ggtgaagcaa
     7021 aatggacggt tgaaacagcg ctagaaatgg ctgttccagt tccagtaatt gcaactagtt
     7081 tgttcatgag gaatctttca caggaagaag atagtttttc agcaaaagta gtttcagcac
     7141 taagaaatgg gtttggcgga catgaaatag ttgaaaaggg tgagtaaaat gccagattat
     7201 catatagcaa tcgttaattc cagcagtttc ggaagagtct ttccacacca cttggaaaga
     7261 ttaaagtcag ttggagacgt tcatttcttt actgttgatc aagagattcc aggaaaagaa
     7321 ctagcggagt tacttaaagg attcaatatc attattgcaa gtgttacgcc attttttcga
     7381 cgagattttt ttgaaaacaa agatgaactg ctattgattt cccgacatgg tattggttac
     7441 aacaatattg atttaaaagc agcaaaagaa caccagacaa tcgtatctat catacctgct
     7501 ttagttgaac gaaatgctgt agcagaaaat aatataacta atttgttgtc catgcttcga
     7561 cgcacgacac aagcacaagc aagagtcaag gacgatcgat gggaagatcg ggcggagttc
     7621 gtcggtagga cgctgtttaa caaaacagtt ggtgtgattg gtgcaggaaa taccggaagc
     7681 ggagtaatcg aaattttgcg taacggtttt cgctgtgatg ttctagctta tgatccttac
     7741 aaatcagatt tatacatcga aagctttggt gcaaaaaaag tagaacttca agaattactg
     7801 gaacaatcag atatcatttg tttatgtgcg aatttgacag aagagaacta tcacatgatt
     7861 tctgataaag aagtcgatca gatgaaagaa aatgtttatc tatctaatag tgcaagaggc
     7921 gctctagttg atgaagaagc gatgattaat ggtctgaaaa gcggaaagat cgcaggatat
     7981 gccacagatg ttttagaaga agaaccggga cgtcaagatc atccgtattt agcgtttgaa
     8041 caagtagtga ttacgccaca tatatcggcc tacacaaggg aatgtttaga agcaatgggt
     8101 gaaaaatgtg tcagagatgt cgaacagatc agtcagcacc ttcttcctga acgttctgtc
     8161 cagacaaaga gcaactatat aaaaggatga tgaagaaaat ggtgaacggt ttaaaagtga
     8221 aaacaggccc tcaattttat ttatatgaag aaggtggtat ttcaaaagtt tctgatctct
     8281 tgaaatcata cggcgcgaaa agagtactgg tcacccacgg aacggtatca tgggaaaagg
     8341 cgctacctaa attagtattt ttgaatgacg aaacgattca atttttttat catcgttatt
     8401 ctggagaatg tagttatgct gaagccagac gaatagctac aatcatcaaa aaaatgaaat
     8461 cgatttcgta gttggcgttg gcggaggaaa actgaccgat ctagtgggtt atagtgccaa
     8521 tcttgcaaga atcgatttcg gagtcatccc tactttagct agtaattgtg ctccatggac
     8581 acctcttgct gtcatgtata aggagaatgg acaagcagaa gggaaaacag aacactttta
     8641 cagacaagct gcttttttta taacagatcc ccaattagtg attgattccc ctgttccata
     8701 tttcattgcc gggttagcag atacactagc aaaatggtat gaatctgagc tcatcttaga
     8761 acaagatttt ttgcaagatg aatcattttt gaagctagca caagatactg cgaagatctg
     8821 caaagaagag attttgaatc attctgctaa agcaatccaa gatatggcac aaagaaaact
     8881 gacatcggag ttcaaacagc tttcagagat tatctttgct gttgctggtt tagttggagg
     8941 attaggagat aaatacgcaa gaaatgtggc agcacatgct atgcatgacg ctatcagtaa
     9001 atatattccg gaaagccatc gttttctaca tggagaaaaa gtagcgtatg ggattttcta
     9061 tcaactggca ctagaaggtc gctgggaaaa aatagacgag ttatatcctt tgtatcggga
     9121 attagaactg ccggtttctc tagaacaaat gggattattt ccgaaagata aacagataat
     9181 agataaaatg gctgaattta ttgattcgaa agagaaagtg catttgattc ctatagaggt
     9241 aacacagaaa tcactgatac agtcaatgat ggaactggaa ggttatatca gaaagatctg
     9301 aaggaggagg acacatggaa acattaagtg tagtacaaag tttgttgatt gctttatggg
     9361 tagccgcggt tatgtcccgc tggttaggtg gaggagccac gttgacctta cgtttttctc
     9421 ctttgatgac tggattagtc gtcgggttga tcatggggga agttgcacaa gcgatgattg
     9481 ttacagccgc tttgcaaatg atttatatgg gggttttttc tccaggagga tcaatgccag
     9541 ctgaaccatc tattgcagca gctatcgcag taccagtcgc attattaggg gatctgtcgc
     9601 cagaagcagc gattgccgtt gcggtgccag tcggattgct cggaagttac ttgtatcagt
     9661 ttcgtttttt catcaatact tttttaggta aatatacaga caaagctgta gaagatttga
     9721 atacaaaagc tatggcccga tcaattattc tttatccaac ggttgcctca tttttattat
     9781 tcgttccgct tgttttcatt gctttatatt taggggcgcc attgatcgct gatgtaatca
     9841 aagctttaga gggaacagtc gttatccaca ttcttgaagt agtaggtgga gggttagcgg
     9901 caatcggtat cgcgacaact gtatacgtta tcggtcgaaa agattatctg gtctttttct
     9961 tccttgctta ttttatgagt gttgttttga aatctttaga tattacgatg gttacttatg
    10021 ccatttttgg gattattatc gctttgatct ttgtccagtc gcaaaaaggg aaacaagtac
    10081 agcctgccac aagcaataac aattcagcat cttttgatga cgaagatgac tatgatgacg
    10141 gattttaatt taggaggaat gggaaatggc tgaaataatg gataatcaaa caggtgctcc
    10201 tgaagaaatc acgaaaaaag atgtaacaaa agcctatctg agatggcatt ttgcaaacga
    10261 aattcctcac tcttttgaac gttacttggc gccatcacta ctatacggaa tgatgccaat
    10321 cttgaaaaaa ctttataaag atgaagactc tttaaaagca gcatataaac ggcagttact
    10381 gttcttcaac actcaattaa gctggggtgg cggtgtgatt actggcctaa tgtcttctat
    10441 ggaacagcaa agagcagaag aagagtatga agaaaaagag atcttgatgc aggatgactt
    10501 gatgtataac acgaaagcgg gattgatggg ggcactagca ggaatcggag atgcaatcga
    10561 ttcaggaacc gtccaatata tttttatcgc aatcgcggta ccttgggcac aacagggaag
    10621 tcctttaggc gcactttttc cttttatctg ctttgggttg tatcaggttt tactaggggt
    10681 atttttcgca agacaagctt tcactatggg ccgtaatgct acaggcctaa agcaaaacac
    10741 tggtgttcag acagcgattg aaatgctatc agttttaggg ttgtttatga tgggggtgct
    10801 tgcgggaaac tatgtaaaag tagaatcttc attgaaattt gcgatatccg gacgtgagtt
    10861 tgttatccag gaaattcttg atcaaatcgt gccagggata ttacctttag ctgtcgtaat
    10921 gggtgtgtac ttgttctata cgaaaaaagg attgaaagtc acacaagcat tattatggct
    10981 gactggaata ttaattgtgc tagcaggaat cggtatttta taaaaagaag ggaacgtgaa
    11041 taagatgggt gtagtcaatt tagcgcgtgt ggatgagcgt ttgatccatg ggcaagtaat
    11101 ggtaacgtta tctcaaaaaa gtggagtgaa ttccattttt gttgtagacg aagttgtagc
    11161 aaaagataaa tttatgagag atctttacaa aaatgcagga agccgtacag gacaaaaaac
    11221 aattgtcatt acaccagaga aagctaaatt ctattgggat gaatatcaat ttaaagaata
    11281 caattgtatt ttgatcgcaa aaacagtcca aacgatttac gacttagtaa aacacggaat
    11341 ccccatggaa gaattgaata ttggagggat tgctcaaaaa aatcctgata cagatctttt
    11401 agtgacgaaa tctgtttatt taaataaatc agatgctgaa aaattgaaag aaatgaatga
    11461 acaatatggc gtaaagaata tttattttca agccactcca tctgcaccta aaacaggatt
    11521 aaaagatgta ttgaacaagt ttgatttgtc ataaacaaat gcttagaaaa aagataaaga
    11581 gggcgaaaaa atgaagaaga acgagctgtt tcgtgattgg gaatttcgtt atcgctatat
    11641 ttaccgtaag cgccgtacaa aaaagagtaa acagcgtttt ctttcagcat tagtatcaga
    11701 tatttacagt atgagaacag atgtgacagt cattgcttac gatacgctag cctaccgatc
    11761 aaaaaatatt tatgttggag atatcgaaaa agcagagaaa gttatctgta cttattacga
    11821 tacacccgtt catgcattag gaagttattt catgtttgat tggaaagacc aaaggaaaaa
    11881 aacaatctac tctatattat tgtccttcat tttgttgttc agtttaggtt ggtggggaat
    11941 gatgatatat aacaaaaatc ctcatcacgt attcgatctg ctatccgtat aaacaggtat
    12001 cactgtacta gcatttggga gctacttttt cttattggga aaagcagctc gcggatggcc
    12061 tagcagacaa acttttattc gaaatacttc atccatatta acgatgctag aaatgatccg
    12121 tacgattgat gatccgagtg tagcgtatgc gtttgttgat gaaggctgtt atggagaaaa
    12181 gggcttagac agcgtgagat cgggtatgaa aaaagaggct attctttttt atctagactc
    12241 tgttggggca gatacaccgc tacagttttc gggaaattat ttttcaaata aagaacagtg
    12301 gctgaaacaa gttgataagt tgaaagaaaa aaacgtcaat tatattttta gtgcaagaaa
    12361 aaagcaagcg cagttcttct atttgaccaa aacagattta cgagggaaaa catttaattg
    12421 gcaaaatgcc aatcagatta ttgctctttt tcgataagta aaaagtgcaa aacaaggagt
    12481 gagaagacta atgttggaaa tcatgatcgt tacacatggt aagttgagcg aaggactgaa
    12541 agatgcggcg gaagtaattg tcgggacatc gagccatatt gaaacaatgt gcttagagtc
    12601 tggggaggac atccaaacat tcggagagaa ggtcaaagaa aaattaacta tgatcaacac
    12661 agcagaaccc atgctagtat ttgttgatct aattagcgca agtccgtaca atcaagcgct
    12721 cttggcagtc aatgaactga ctgaagaaaa gaaagcagcc acttatgtca tagggggcgt
    12781 gaatctgcca atgttgttag aagcaatcaa tcatcagatg cttcacacac cgatagaaga
    12841 agcggtggaa caaattgtca aacaaggaaa agataattta actgtttggc atgcttcagc
    12901 ggaagaaaaa aaattagatg aggacgattt ttagaaggaa ggctgaaaag atgaagtggg
    12961 gatttagatg gtatggtgaa aaaggggatt cgatcccatt aaaatatgtt cgccaaatac
    13021 cgggaataaa tgggataatt ggaactttgc tgactaaaat gcctggagat gtttgggaga
    13081 tttcagaaat tgaggagttg aaagcatctg ttgagagcca aggactggaa ctgcttggaa
    13141 ttgaaagtgt agcgatccac gatgcaatca aggcagggac agatgaacga gattactata
    13201 ttgataacta tatacgaaca atcaaaaact tgtctcgatg tggggttcga ttgatttgtt
    13261 atagcttcaa accaattttt ggatgggcga aaactgattt gtttaatgaa aacaacgatg
    13321 gaagctattc tcttttatac gatcagaaaa tcgtcgatca gatggagcca ggagaaatgt
    13381 atcaattgat acacagccag tcaaagggat tcaaattgcc gggttgggaa gaagaacggt
    13441 tgaaaaaatt cgctcagctg gaaaaactgt atgaaggaat gaccgaagaa gaattatttg
    13501 aaaatctgac ttatttttta gaaaaagtga ttccagtgtg cgaaaaagca gatgtaaaaa
    13561 tggctatcca tccagatgat ccgccatggg aactttttgg ttttcctaga gtgacgaaaa
    13621 atcttggcga cttgaagaaa attctaaatg cagtagattc tccatataat ggactaacgc
    13681 tgtgcacagg atctttaggg gcaaatccgg aaaacgattt agttgaaatc atccatgagg
    13741 taggttcgcg catccatttt gtccacttca gaaacgtgga gtttttagga gaaagaaagt
    13801 tcaaagaaac ggcacatccc tctacagaag gctcccttga tatgtatcat attatgaaag
    13861 cgttggtgca agaagggttc gacggtccta tacgccctga tcatggtaga accgtgtggg
    13921 gtgaagtggc tatgcctgga tatggcctgt atgatcgtgc aatgggactc acttacatgc
    13981 aaggtctata tgaggcgata agtaaggaaa aaccactggg agagcattat gaataaaatc
    14041 aagctcttca tctttgatat ggatggcctt ttattagaaa ctggacgttt agcttatcgt
    14101 gcttatgtaa aatcagcaca aaagtatgat tatgaaatgc gtaaggaagt ctattattta
    14161 ctaactggcc aaacagaaat ggcgataaga gaacaaatgg gcattttgta cggagaagat
    14221 gttccatata tcaaatggcg agaagcaatc aatcaatata aagaaaaaat cgtgaaagaa
    14281 gataagagag tatatacgaa aaaaggagca gaagaaattt tatcttttgc aaaagaaaga
    14341 ggaatccata caatcgtggc ttcgtctaat acacgggtaa aagtagaaat gtatttaaaa
    14401 atggaaaatc tatatgcctt gtttgatcaa atcatttcag gggatgatgt aaaaaaagga
    14461 aagccggaac cagagatctt tttgaaagct tgttcaaaaa tgaatattcc tccaagtgaa
    14521 gcacttgtat ttgaagactc gattgcaggg atcgaagcag caaggcgagc gggcattttg
    14581 tcttttctca tcaaagatca tttgataggg ttgcccgatc ataatgggaa gcataagttg
    14641 aaagtagatg taagtttata tgaatccaat aaagcagact atgaatttta tgacctgcat
    14701 caagcaaaac gatttttaga aagtaaaggg ttgtatctct aataaagagg actattttct
    14761 agtcctcttt atttattgtc ctgcacgact gagggaacag atgaaaaaga agtaattttt
    14821 tattttgaag tatctacctt atttgtagtg aacaatttag aacaaacaca aaacgcatct
    14881 actgttatct accagtagat gcgtttaaag agaagaacga gaatcgtcca tatgagggtt
    14941 tccactcctt atgcagattt ttttaattct tgcactgctt gttcgatcgt aacacctgtc
    15001 gctacatgat tggtattttc tttgtcaaaa acgacaaata gattcaaatc tgtatccaag
    15061 gttactcgat attgtatatc tttgttatag aatgtcatag ccattctcct ttctttaaat
    15121 acttgctgaa aggcaacgaa cgcgttgata acgaaattcc atttataagt ataccaccaa
    15181 ttgaaaggtt ttcatagtta agaaatttta aagtcaaaag ttgcagataa aagcaaataa
    15241 acaagaaaac aaaaagtgaa tcaatcgcgg ttattcaact ttttcttcga ttcgaagaca
    15301 atagaagaca atgaatttta tatttcattg cattatcctt gtgttattag aaaaagcatg
    15361 atatattaaa tgagtaatct attttgaaac gtaatttgag cgatatatct acatagataa
    15421 aactcctttt gcctatcaaa tatttattgc aacgaaaaaa cgtacattga tacgtatcag
    15481 gaggaattta tttatgaata acggtacagt aaaatggttc aacgcagaca aaggttttgg
    15541 ctttatcact ggtgaagatg gaaatgatgt atttgcacat ttttcagcta tccaaggtga
    15601 cggcttcaaa actttagaag aaggccaagc agtgtcttac gatgttgaag atggtcaacg
    15661 tggccctcaa gcaacaaaca ttgttaaagc ttaataaaaa ttgactttta accgtctttt
    15721 cgaaagaaaa gacggttttt ttgtacgaaa aaattcattg gtcgttgaat gggctgaata
    15781 gtagtgtata aaacaatttt gtggagcgtg aagcttttat ggtagaggaa aatacgaata
    15841 aagtgatgtt gataaaaaga ttcctatctt gaatatgaat cgagatgtct tgaaaatgaa
    15901 tgagtttgac caatcattat cctatcgaaa tttacagttt cgttcgattg gtctgtcaat
    15961 taatgaaaaa gtaaaaaaga atatgttcta ggaagagggc ggcacatcta aatttttaag
    16021 taacggacag ttcaaatatc aagcattgaa agaggctatc ctactgtttg aaggaatacg
    16081 aatatataaa aacgacaaag aggaaaaagt agttcattaa atcccttcaa gtgatgaaag
    16141 aatgagatct aataaaacct gatctttcat ctgttttttt aataacgtaa taattttttt
    16201 attttttgtg ctttcgccgt tattgtagag tgatttagca ttcttatttt taaaaataac
    16261 tgtgttatat attaaaaaag gcataatagt tataagcttt tttaaagaat atttagaaat
    16321 tattaatttt cgtctgaaaa caaaaaaaga aagcgttatt attttagtct attttcaggg
    16381 ggggaacctt atgataaaaa aactattttt actatcaata ttaacgctag caatggcagg
    16441 ttgttctgac tctggcttag caactgaact gatcacaaaa tcaactgaag ccacgacaga
    16501 ggagttaaaa tgtggaaacg atcacagtga ctacagaggc ttaaatcgac gatgcatggg
    16561 aatattaggc agaagcaatt gtaagcatcg atgtagtaat cgaagaaggt gtagaagaag
    16621 caacagaaaa atcaaaagaa agatcaaaag aagcaatctg tgctgtgcat cgtatacaga
    16681 cgtcgaagat gcggtacttt taaaccacgt caggagatgt tcatttatgt ctagggaagt
    16741 tggcgatgaa ccaaaactag agtacgcttt tagttttgca cgtgaggatg aagataaccc
    16801 taaaacttac gacattttag cttgatctaa gatggttaaa gctttttaat gaagtaagag
    16861 gagatagaag gcttttctag ttatattagg taagataaat tcctttttgc tccaaatttg
    16921 ctaaacattc tgctagataa acttcatcgt gttccggata aatcggcacg ttgcgctcta
    16981 cagtaggtaa atcaaaaaat gcttcgcatg atttgccacg atacatagga tttttccact
    17041 ctggtgctgg gctgtaacca cgtttttcca tttcatccat gacaagctga tgatattgga
    17101 ataatctata aggagaatag tgaaaaacat aatccacagt agcatgtttc tttccccagc
    17161 catttcctct taatgcacag cattcccgat gttgtcctaa aagctgttga cgaggaagct
    17221 ttgaaatcaa tttttcatgc cataatcgca tagaaatgtc tcctttatat atagattgtt
    17281 aaccttagac gttaaatgaa aaaaccagca ccaaggctgg aaaaaatcac ttcagtttgc
    17341 ttgctgtttc tgagcgacca attaggtaat caacggaaat atcgtaaaag tcagcgatat
    17401 agattaattc aatgatgctt ggaatttctt ctccttcttc gtacttacga tatttgaata
    17461 aaggaatact taacgcgcta gaaacagcta cttgagataa agatcggcca gttcttaaga
    17521 aattcagacg acaacgtaaa gtatccagta aaatagcttc ttcgatactc atttaatcac
    17581 ctttcattat agtagaatag ttataatgac tatagaaagc tacacatgca atcaatagta
    17641 ctcaatgttt tttatttaaa cgtatcaaat aaagaatgac gatcaataaa gacaaaacca
    17701 gtcctgataa ttcttctata aaaacactat cgggttcaaa gtagttgtaa ttaaaaactt
    17761 tccacataaa gtagatagcc ggaagagtca aaatagctaa ataaaaccgt tctttgttcc
    17821 aattcataag ataaacaata cttgcgacca gtgcgaccgc aggcacccaa aacaaaactc
    17881 tcaccaacgt aatcatgtaa cggactcctc aacgattcat ataataaaag ataacatgtt
    17941 attgcataca tcacaaatga aatgctagga aaataaaaga agaatagatc aatgaaaaat
    18001 gaaaaagcag aacagttttc tgctgataag caggaactgt tccgctgaat gtgtcttatc
    18061 tatcgagaaa aaatcaatgg attgatatct aagtaattca atacatataa aaagaggacg
    18121 atcgcattca ctaaaaagga aagaatgatg atcccatagc gaatggaaac atatttaaca
    18181 aatacttttg tttgaatacg ggcatatctt tttcctttat agctgagcat catgacaaga
    18241 agcaaaaggg taatcacacc ttgaaaaatc attccaataa acagaattcc tgaggtttga
    18301 taatttaatt ggagaaaata agctgctgcc gcaactaaat cgaataaaat cgcaataaat
    18361 ggcaaaataa tcactcctaa cattgagaat aaataaaaga ataacatttt ttctaaaata
    18421 agcaaataaa aattaaagta acaaaatagt tatgacacaa gtaaaagaaa taagaataac
    18481 aatattattt tacttatatt ttgaaacttg tgagagtttg tcattcgtgc tatagttgaa
    18541 aagtaacaaa aataaatttt atttatgatg tacctttagc tcagttggtt agagcagacg
    18601 gctcataacc gtccggtcgt aggttcgagt cctacaaggt acataagagg ttgtgaaaaa
    18661 gctgccctgc atcaagtaat aagaacagcc aagtaaaaac agcttcttat gtttttactt
    18721 ggctgttctt attactgcag atgcaagctt ttgaataccg tttaaggaag aggttgtgaa
    18781 atcagcgttt tgacctgagt attagcttaa caaacggaaa aatagttcct catattttgc
    18841 cgtttgttaa gctaataccg agggtcagct gattgaacac catttaagaa agaggtcgtg
    18901 agcctgccgt ttcactctgt gtcataaaaa agccaatcta ctttgataga aaaagcacgt
    18961 aggactaaaa cagttctgcg tgcttttttt attgtgtgtt aaatatcgta aatgaagata
    19021 gatattttta tgattcgacg atagagacgt tgaaatcttt caacagctct aaagcggatt
    19081 ctccgtgttt tctactggag actaacattg gatcaacaaa aaagtctgct tcaggaaaag
    19141 cactctgcag gcctatcaga ttagagagta cacacaagtt tgtttcaatc ccgactactt
    19201 cgatctcttt ttgttcttct ttgctttcaa ataaagtttc ctggatttca gctaaagttt
    19261 caggtggaat cgtaaagtaa tactttttaa tcactcgttc attttcttgt ggagaaagaa
    19321 gagggatcaa ttgcaaatct tctcgttctt cttctttgtc cttcagttca actggtatat
    19381 cccgtgtgaa gagaatatat tcattatttt ggcgtgcttt agctaatctt tgagcaatac
    19441 gttctgccaa ttgatctgac tctggaatat aaaattcgct gtttggatca agaatatgat
    19501 tttgcatatc aataataact aacattttct ttcctcctac attcatatcg tcattttatt
    19561 ctttttaata gaagtttttt aaaatataag caacaccagc ttcttcattc gtcagtgtta
    19621 cttggtttgc aatttctttt acttgtcgct gtgcattccc catcgcaata ctcaacccgc
    19681 tgtaattcaa catagcaatg tcgttttctt gatcgccaaa agccatgatg tcactcgatt
    19741 cgattccaag gtaatccgcc agaaaaacaa gtgaatgagc tttagtgata cctgtaggcg
    19801 tgatttctaa gaaatgcgga tcagagaata ctgattggat accatctgct tgcagatctc
    19861 tttgtagctt ctgtagtttt tcttcactct tatctgttaa tgctaattta agatagctaa
    19921 gagaaggatc ttccaacata tttaaaattt gatcaaaaga atggttctgt aaattgaaat
    19981 gttcataata agggtcatct ttgactagat ttgattcatc gaatgtcagc tgatcatctt
    20041 gggtttctcg gctagcaatg atggatattt tcgcttcttg agcatagaag aaggcttttc
    20101 tcagtgtatc gctatctatt tttgaacgtc tgagaacttt cgccattttt ccatccttca
    20161 tttcagcaat cagtgaacca ttggaagaaa tcaaatagcc accgattcct aattcggaaa
    20221 aaatatcttt agcagaaatg ctgttccttc cggtagcgac ggttacgatt ccgccatttt
    20281 catcaaattt ccgaagtgct tggatatttt cttctggtat ctttccattt cgatcaagca
    20341 aggtaccgtc taaatcaatc gctgctagtt tcattttatc ttcctacttt ctttttacat
    20401 atctattttc ctgttacctt aagattaaat gagaatttaa aaaaatgaaa ctaacttgct
    20461 ttgtcattat gaattttttc aaaaaacatt cttaagaaaa aggtggctat atatagtagt
    20521 ttttggtcaa tacctttgaa taaaaagcca tgaaaaacga atagttagcg cttaccgatg
    20581 cgttttgtga taaaaaaatt ttaaaatatt caatgaaaac aaagcatata ttttgcttta
    20641 aaaccaaatg aggaatattc tagatgtaga acaggttaga aaaagattag aaaaaggagt
    20701 gattttgatg gagaagagat acggcgtagt tcctgtggta actgcttcag acgaaaatta
    20761 tgctccttac ttgagcgtga tgatcgcaac tgcattagaa aattgcaata aaacaagacg
    20821 cataaaattt tatgtgattg acgatggtct atcagaatat agtaaagaag gattggaaga
    20881 aacggtaaat aaatattcaa gtaatgcaag tatccaattc ctgacagtgg aaaaagatat
    20941 ctacgaagac ttcctagtca gtgaccatat cacaactacc gcttacttaa gaatttcttt
    21001 accaaactta ttagctaaag aggattataa gaaagtcctt tacttagatt cagatgtgtt
    21061 agtactagac gatatcgtaa aactatatga tgagccgtta aatggaaaaa caatcggcgc
    21121 tattatcgat ccaggccaag tgaaagcttt ggaaagatta ggaatcgatt ccgatgactt
    21181 gtacttcaat tcaggagtga tggtgattga cattgatcaa tggaataaaa aagaaattac
    21241 agaaaaaact atccattacc tcagtgaaaa tggcgatcga atcatctatc acgaccaaga
    21301 tgctttgaat gctgtacttt atgaagattg ggaacaactt catccaaaat ggaacatgca
    21361 gacttcgctg atttttgaac gtcatccagc accaaatgaa aaatatgaac gtcagtataa
    21421 agaaggaaat gaaaaacctt cgatcgttca ctttacagga catgataaac cttggaatac
    21481 gttgaaagat catccgtata ccaatttata cttaaaaaaa ttagcacaca gtactctaac
    21541 gaaagtaggc gagatcaatg aataaaaaag aaatagcagt agtcgcaagt tgtaacacaa
    21601 aattcgtacc acatttagct gcattatttg tctcagtatt agataattgc aacccgtcta
    21661 aatttgttcg gttctatgtg attgacgatg atatcgattt tgaaagtaaa cagcttttac
    21721 gcttctcagt gaaaaacgca cgaatgaact cagatgtgga attcttgaaa atcaataaag
    21781 aattctttac aaatgtggta atcagcgatc gtattccaga aacagcttat tatcgtattg
    21841 cgattccgga attgttccgc ggcacagaag ttgaacgtat cttatatatg gattgcgaca
    21901 tgatcgcttt acaagatatc tctaaattat ggcgtcttga cttcggtgat tcaatcgtag
    21961 cagctgttga agacgctggg ttccatcaac gactggaaaa aatgaaaatt tcagctaaat
    22021 caatgcgtta tttcaactca ggtttgatgc tgatcaatgt gaaaaaatgg ttagatgaaa
    22081 atattacgca aaaagtatta gatttcatcg aacataatcc agaaaaatta cgattccatg
    22141 accaagatgc attaaatgct atcttacatg accgttggtt accacttcat ccgcgttgga
    22201 atgcacaagg ctatatcatg gcaaaagcaa aaaaacatcc aacagcagca ggtgaacgtg
    22261 aatacgaaga aactcgcaac aatccttaca ttatccattt ttcaggacat gtgaaaccgt
    22321 ggagcaagga ttttgaaggg ccaacgaaga aatattacga aaaatatgca gggatgacgg
    22381 ctttccgttg tgtagctaaa ttccctaaat atccaaaata tgcaaaagtt caaccaaaaa
    22441 cagatgtagt tgttaaataa tagaagacaa caactgaaga gggggagtag gaatggaaat
    22501 tacaatgtct aaaaatgcag tagagacgtt aattgaaaag gttggggaaa atacaaaaat
    22561 cgcactcgcg cttatcaatg attccgatcc atttctaagg gacaaaggtg cttttgcaaa
    22621 aggcagcttt tttcaaatca ttccttttgt ttcggaattt ggagaatatg cgactaaaat
    22681 cgagcatcca ttattggaca tctatacatc taaattagag caaaattatt tcggtaaacg
    22741 attgaacatg gacttcaaca aacagcttga cagcttttca ttagaaaatg aaatcgcagt
    22801 actcgattat aatatcaaat taaagaactg cttcttcagc taatagtttc gagaaagtcc
    22861 aaaaaggagg tatacattga gatactactt tgtggatgaa gaaaagaatg taatagaaac
    22921 agatgaaaaa aaatataatt ggctcgtact tgatagtgca aagcccgaag agattgagca
    22981 agcaatcgaa gaattcgagc ttcctgatga tattttcgta ggaaccgaat atccggaaga
    23041 agtttctcgg atggagcgcc tttatgagac gaaactagag catccatttt ctttggtagt
    23101 gattaatcta gatcagtcag ataatcgtat cgaaaaaaat taacgccaat atcttttgta
    23161 ctttccgata atctcttgat tacttgtatc gatagaaaaa ccgaatttgt ggatcggctg
    23221 ctgacaaagc atggaaatca tttaaactcg tttgaaaaga tcatcacgta tacattatta
    23281 gatatttaca cccattacgt gaaagaacta agagagatga aaaagcgaat tgacgctttg
    23341 gataaagcag ctcgtaaaac aacggaaaat gaagaactgt ataaacaggc agatcttgaa
    23401 agagacatcg tatatatcga tcatacctta agagatcaac gggaaacaat ggatcaatta
    23461 tggaattctg aaactttcat caaacgttta gacgatgaac agctactcta tgatgtccat
    23521 ttgagacagc gacaaacaga aaaaatgatc gagatctatc gagatttatt agagagtatt
    23581 ggtgatctat tcaacgggat gatggacaat aatctaaatc atatcatgaa atatctagat
    23641 tcagctgcat taatcatttc cgttccggcg ttgttcgcag ggatttgggg aatgaataca
    23701 ggaggattac cgggtaaaag ttctgctcta ggctttatac tcgtagtctt tggctctttg
    23761 atagcagcag tagttttagg ttaccattta taccggaaag attatacaaa ttaaagaatc
    23821 tatttaaaac aggtatcaaa caaagaaatt tgttttggca atacaattta aaaactagga
    23881 ggaatacagc aatgacagaa ccaaaattaa tcgacccgcg aaacttgtac catacggaga
    23941 aatttcctaa acaagatcag gatacaccag ctttacagca gaatatgatt cctgttcctg
    24001 attgtggaga agaatcctat gaaggcaata atcaactaga aaaccgccgt gtactaatca
    24061 ctggtggaga ttcagggatc ggtcgtgcag cagcgattgc atttgctaga gaaggggcag
    24121 atatcgctct tcaattcttc ccaggagaag aaaaagatgc agaggaagta gcaaaatacg
    24181 tgaaagttgc aggacgtaag attgtcttac taccttttga tcttcgtgac gaacaagcac
    24241 ctaaagaaat catagataaa gccgtgagtg aacttggcgg gctagatacg atggtactaa
    24301 acgcaggaca gcaaatttct tgtcagtcta ttgctgagtt accacttgag caagtcaaag
    24361 acacatttat gataaacatc atcgctatgt ttgctttagt gaaagaagct gttccgcata
    24421 tcccagcagg aggagcaatc gtaacaacaa cttctgttca agcattcaat ccaagcgaac
    24481 atttattaga ttacgcagca acaaaagctt caatcgctaa ctttacgatc ggtttagcaa
    24541 aacagcttgc acctaaagga atccgtgtaa atggtgtagc acctggtcca atctggacac
    24601 cattacaact agacgaagga caaccttctg attcgatccc tgaatttggt cagcaaagtt
    24661 tactagaacg tgcaggtcag ccaggagaat tagcaccagt ttatgttttc ttagcatcga
    24721 ataaagcaag ttatgttaca gcccaaattt acggagtaac tggtggagaa gcgatcaact
    24781 tataagaagt tgatcgtttc acccctatga ggaggtgcga aaatgaataa aggtgtaaaa
    24841 acgatcggag tgatcggaac attacttttg ctatctgtgc ttttgtttgc gatactatta
    24901 agtgcaacct atccattgcc ttttcagcta gagaagtttc gattcttttc catcactaat
    24961 tcatacgtgc aacaatatgt attttggctt gccgttgcat ttgctatttt gacaattatc
    25021 acgttacttg ttttcctgtt ttatccaaaa acaatcggaa actttctact gaaacatggt
    25081 gatggggagt tgacgttgga taaaaaagca attgaaggaa tggttcgttc tcacttgcat
    25141 gaagatgaat tcatccattc accaaaagta aatgtcaaag caacgaaaaa caagatcgcc
    25201 gtcaatgtaa aaggggagtt gaaacgaact tcagcactca ttggtaaaac aggaacattg
    25261 atgaaagaaa tcgaaaatga agtacaacac atattgggaa caaaagagcc agtgaaagta
    25321 tcggtgaaat acacaggctt tgaagaacca gctaaacgaa gcaatgaccg caaacgagta
    25381 gaataggggg ggaccaaaaa tgaaagaact attttcgaca tacaagctcc ctatcatctt
    25441 tggggtactt ggattgatat tagccgtttt atggatcacc gtgggctttt tcaagacctt
    25501 attgatcttg atcatggcag cgatcggcgt cgctatcggt ttttatctga aacaaacgaa
    25561 aatcttagat cactatttta aatcttagga ggaaacatcc atgaataaca ataacaaaaa
    25621 taataacaat aaaatgaaca accaaaaacc agtgtctgca catccagacc caacgacacc
    25681 aaaccaatca aatggtgtga acgatgaaaa gaacaaaaca gcaccagtat ctgcacatcc
    25741 agatccagta gtccctaacg ttcaacaagg agtagaagag aagaaacatg atgcagaaaa
    25801 gcatgcacct catcatccag gaactccaga atctaaagtt ccagaacaac atgatgcaat
    25861 cgatccttct aaacctgtgg aagagcaaaa acatccttct gaccatgcga aagcacaaga
    25921 aaaacttagt tctgaccaca caaacggaca aggaaaactt ggttctgatc atacaaataa
    25981 ccaaggaaag catgattctg aacaggaaga ttcaatgcgc ggtgagctga attatgaaga
    26041 taaagttgtg caaaaaatca tcggaatcgc aattgaacaa gtagacggtt tattatctgc
    26101 aaacggtggt ttcttctcta atgtggcagg aaaacttgtg aatacagata atgtaacggc
    26161 tgggatcgaa acagaagtcg gtaaaaaaca agtagcagtt gatatggatg tcattgtgga
    26221 atatggcaaa gatatcgaaa aaatctttga agaaatgcaa gaagtaatcg gcaaagaagt
    26281 aaaaaatatg acacatttag aagtgatcga agtcaacgct aacgtagtgg acatcaaaac
    26341 aaaagaagaa tttgaaaaag atcaagaaac ggtgcaagac aaagtaactg acgcagcgaa
    26401 aaaaaccggt gaatttgctt cgaatcaaac agataaagta aaatcagcag ctggaagcgg
    26461 cgcacaaaaa gtaaaagaaa atacagaacc acgggttcag taataaggaa gtaaaacatt
    26521 aggaggaacg aaacatggga tttatttggt cacttatcgt cggaggaatt atcggagcaa
    26581 ttgcaggagc aatcacgaat aaaggttctt cgatggggat tattgcaaat gtcgtagcag
    26641 gtttagttgg atcagcactt ggtcaagcat tattaggttc ttggggacca agtattgcag
    26701 gaatggcaat cgtgccatca ttgattggtg ccatcatttt agtactaatc gtatcattct
    26761 ttgctcgaaa actgtaaaaa gacttttaaa aagaggtcgt gaaaaagctg ttcttcatca
    26821 agtaataaga acagccaaac aaaaatagct tctcatattt ttcatttggt tgggcatatt
    26881 atcgaagatg caagcttttg aacactgttt atacggaata agaggctgtg acaagacttt
    26941 tgtcacagcc tcttatttat gcgggcaaat tcccaattag atttttaaaa gtttttcctt
    27001 aaatgtgtct ttattgatac agagaagctt ctttaataaa acaatgcctc cctaaatttt
    27061 cttgagatag gaaaggtgaa catacaaaat aatacgacca ctttgaaaac ccagatgatc
    27121 cacaaatgga tcgtatgata ctgatgaagt aaaaagcaat ggatcagaaa ctcaaagaga
    27181 ttgaaaaaga caaggaagaa ataatatctc ttttttactc ttgtctgttt tagccaataa
    27241 tagctcgtaa agtaatctag acaaacaaat agaagtaaac aaagtttcgt aagagcagca
    27301 atcgtatcat gttgctctgc tagacgattg acgagcagga aaaatgttgg taatggaata
    27361 tagatcacga gtaatgaaag aaaaaagtaa acaataaatg gcatactatt cctcctaatt
    27421 gtgtgagttc gccctcattt taaaataacg atatttgttt gtgaagcttt ttcgtgtcgt
    27481 aacaaatgtt atctaatgta tttttaaata aataatcatt attatcaaaa taat
//
LOCUS       DACEEZ010000027        26615 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20313_2017
            SAMN12639966-rid9879223.denovo.027, whole genome shotgun sequence.
ACCESSION   DACEEZ010000027 DACEEZ010000000
VERSION     DACEEZ010000027.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639966
            Sequence Read Archive: SRR10023158
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 26615)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26615)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741683.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741683.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:44:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,016
            CDSs (total)                      :: 2,962
            Genes (coding)                    :: 2,889
            CDSs (with protein)               :: 2,889
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 36 of 73
            Pseudo Genes (incomplete)         :: 29 of 73
            Pseudo Genes (internal stop)      :: 18 of 73
            Pseudo Genes (multiple problems)  :: 8 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26615
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639966-rid9879223.denovo.027"
                     /isolate="E20313_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            257..1450
                     /locus_tag="IWO89_001251"
     CDS             257..1450
                     /locus_tag="IWO89_001251"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HAQ0456019.1"
                     /translation="MVERHLFTSESVSEGHPDKIADQISDAILDAILKQDPTARVACE
                     TSVTTGLVLVFGEISTTAYVDIQKVVRETIKEIGYTRAKFGFDGDTAAVLVAIDEQSP
                     DIAQGVDEALEIRDEDKKDVLDEIGAGDQGLMFGFAVDETPELMPLPIALSHRLVRRL
                     ADLRKSNELTYLRPDAKSQVTVEYDDQGQPERVDTIVISTQHDDAVDNETIRHDVIEK
                     VVKEVIPAELLDDQTKYYINPTGRFVIGGPQGDAGLTGRKIIVDTYGGYARHGGGAFS
                     GKDATKVDRSASYAARYIAKNIVAAGLARKAEVQLAYAIGVAQPVSISINTFGTGTVP
                     EEELIAAVRENFDLRPAGIIEMLDLRRPIYKQTAAYGHFGRTDVDLPWEHTDKVDALK
                     ASLAK"
     gene            1580..3052
                     /locus_tag="IWO89_001252"
     CDS             1580..3052
                     /locus_tag="IWO89_001252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAQ0456020.1"
                     /translation="MKKETNVTLVTVSIFIATFMTAIEGTIVTTAMPTIVGSLHGMEI
                     MNWVFSIYLLTNAMLTPIYGKLADKVGRKPIFIFGTLLFIIGSLFCGLSQSMIGLIAA
                     RAIQGMGAGAMMPVALTIIGDLYSIEKRAKVLGLNSSAWGIASVFGPLAGGFIVETIS
                     WHWIFFINVPIGILLIILIWLYLNEPKIIHESKPMDIFGSFSLMAMLVSLLLGFQLLG
                     DEGMSPLVSALLLGSIVMLGIFVFAEKRAKDPVIMMHLFKNPLFVVVNLAAALASGFL
                     MGIDVYIPMWMQGVLGLNAAIGGLVLAPLSLVWMVGSFLASKWMTQMKTHWVLRIGLL
                     IAAIGGAWLYLMPFRSNYLWFFAISTVIGIGLGVTMTTSTVSAQNSVSRDQLGVATSF
                     NTLVRTIGQTVMVSIFGLLINGVTKRELAAANLTNDADIMNKLVNPQTAKYIDADILL
                     PLRKILYDGLHSVYLVGLLLVIIALVLTLFVHEKSGKIKQ"
     gene            3135..3923
                     /locus_tag="IWO89_001253"
     CDS             3135..3923
                     /locus_tag="IWO89_001253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase family protein"
                     /protein_id="HAQ0456021.1"
                     /translation="MAFIQANIYSNVLEMEVNLNVILPQTTKKVIGTSSEQETTDIPV
                     LYLLHGMGGNHSVWERRTSIERYVANYGLAVIMPSTDLGWYTDTVYDMKYWTFIAEEL
                     PEICHELFPQLTRKREKTYAAGLSMGGYGALKLGLAKSENFAAVISLSGAVSVGNRMD
                     DLLMVRKASFWEGIFGPLDQIEGSKNDPQYLLKELVKSGKPVPRFYMACGESDFLYQA
                     NQEMVSAMQEQGLAVTFEQGPGDHNWIFWDQWIQRALAWLMEEK"
     gene            complement(3990..4658)
                     /locus_tag="IWO89_001254"
     CDS             complement(3990..4658)
                     /locus_tag="IWO89_001254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family transcriptional regulator"
                     /protein_id="HAQ0456022.1"
                     /translation="MEIKLSQAIIIQAAFDILAETQALSQLSMRNIANRIHVQAPALY
                     WYFKNKQQLLQIMAETMEEELALPDPTLPWNEQLLQYMENYYDLYTRFPCGAELEINT
                     VPAFPSRLEHLEQMIQLLVREGFSVTQSHQAIASLHNLLIGQLMDQQHEQQLRQKIMD
                     GNEFLKETVLFMRNYVVENQLEGFKESMQSHAELNEKALFLTSAQTYIEGLCQQNKRR
                     ENEQ"
     gene            4773..6485
                     /locus_tag="IWO89_001255"
     CDS             4773..6485
                     /locus_tag="IWO89_001255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ0456023.1"
                     /translation="MKLILRHAKKYKVAVFISLLSVAVMVAAALWQPKLLQQVLEAII
                     TEDNDEMKTIGIYLISLALLGLAAGVTNTIFSAKVAQGVSADIREEAFRKIQTFSFGN
                     IEQFSAGNLVVRLTNDITQIQNLVMISLQSLFRIPFLFIGAFILAMITMPQLWWIIVA
                     LIVTVFLITALSFTRMGKHFMIIQKLIDRVNSIAKENLLGIRVVKSFVQEENQLKSFS
                     KVSEDLTKHNIIVGTLFSVMIPSFMLAANLAVVGAIFFVSDLVKDDPTLIGGIASFMN
                     YLMQIMMAIIIGGMMMMMTSRAAVSLKRIAEILDAEPDLTYLDVPEQELTGSVTFDHV
                     SFRYPGEDTDTLKDISFSIKPGEMVGIVGATGAGKSTLAQLIPRLFDPTEGKVEVGGV
                     DLRQVNEKSLRQTVSFVLQKAILFSGTIAQNLRQGKKNATEKEMEHASSIAQAKEFIE
                     KLSDRYEAPIEERSSNFSGGQKQRLSITRGVIGNPKILILDDSTSALDARSEKLVREA
                     LDRDLKGTTTIVIAQKIASVVKADRILVLDEGRLVGEGTHEELVAGNRVYQEIFETQK
                     GTEE"
     gene            6488..8257
                     /locus_tag="IWO89_001256"
     CDS             6488..8257
                     /locus_tag="IWO89_001256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010762530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ0456024.1"
                     /translation="MTDLIKASKFFYHYLKRYKLSFFFIFITVIIATYLQVKAPQYIG
                     EAFQELANYVGGLMQGVDDKSKFVSVIWKLLLFYVLASAANFIYSILFTQVVGKSTNR
                     MRIGLFNKLEKLTIRFFDSHQDGEILSRFTSDLDNIQNSLNQALLQVITNAVLLVGIL
                     IMMFRQNVELAWATIASTPVAVLIAVLIIRKARKYVDLQQDEVGKLNGYMDEKISGQR
                     VIITNGLQEETIEGFLEHNQSVREATFKGQAYSGLLFPMMQGMSLVNTAIVIFFGGWL
                     ALNGDLERSVALGLVVTFVQYSQQYYQPLMQISSGYSMIQLAITGARRLNEIFDEKDE
                     VKPENCKAFDGVEKGLALQHVDFGYSPEKLVLKDVSINVDKGEMVALVGPTGSGKTTI
                     MNLLNRFYDVNDGSVVIDGIDIRDYDLDALRRHVGIVLQESVLFSGTIKDNITFGKPD
                     ASDEEVINAAKQANIHDFIMTLEKGYDTEISEENNLFSTGQKQLISIARTIITNPSLL
                     ILDEATSNVDTVTEARIQKAMDEAIKGRTSFVIAHRLKTILNADRIVVLRDGEVIEEG
                     SHHELLAENGFYAELYHNQFVFE"
     gene            complement(8462..9028)
                     /locus_tag="IWO89_001257"
     CDS             complement(8462..9028)
                     /locus_tag="IWO89_001257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HAQ0456025.1"
                     /translation="MLQTALHFSHTLLQEILQPGDHVVDATMGNGHDTVFLAEHIGKT
                     GHVYSFDIQQQAIDATRERLEQRQLEERVSLFLQGHETLGEVIAEQQNLKAGIFNLGY
                     LPKSDKAIITMPETTRTAMEEILKRLVPRGRLILVVYYGHEGGEKELDMVDSFCSKLP
                     QETYNVLNYRFINQKNQPPILYCIEKKR"
     gene            complement(9028..9984)
                     /locus_tag="IWO89_001258"
     CDS             complement(9028..9984)
                     /locus_tag="IWO89_001258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390904.1"
                     /note="This family includes YhcC from E. coli K-12, an
                     uncharacterized radical SAM protein; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01212 family radical SAM protein"
                     /protein_id="HAQ0456026.1"
                     /translation="MTFPYSEGNKRYHSWNYALRNEFGGKIFKVPIDGGFDCPNRDGT
                     VAHGGCTFCSVSGSGDMIVAPEDPLPIQFQKEIDMMHQKWPHVQQYIVYFQNFTNTHA
                     PVEIIKERFEQVVNLPGVVGLSVGTRPDCLPDEVVDYLAELNERMYLWVELGLQTTFE
                     ETSKLINRAHDYQTYLDGVAKLRKHNIRVCTHLINGLPGESLEMMKENVRRTILDSDI
                     QGIKLHLMHLMRKTRMLRDYHEGRLQLMSRPDYVNVICDQLEMIPKEIIIHRLTGDAP
                     WDSLIGPMWSLKKWEVLNAIDEELLRRDSFQGKYDVRKKVSV"
     gene            complement(9984..10640)
                     /locus_tag="IWO89_001259"
     CDS             complement(9984..10640)
                     /locus_tag="IWO89_001259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HAQ0456027.1"
                     /translation="MKYKTSFQVAGSFFLVLFFILGWIVKFHLSWLSGFDQWFTGIVR
                     ASYPDLNSYQLFMTKFGNPLTVIILFACAALWMWYKKKRAETFWFVSGFIVIAGIINP
                     VIKLFFMRERPSLAHLVVETSYSFPSGHAAGSMILYGSLLFLLPAFVQKRTLRTLLQL
                     LMMFIIVSVGISRIYLGVHFPTDIIGGYLLSLSWLCLTYPVYREKRKQIDNSYRKKDF
                     "
     gene            11010..13028
                     /locus_tag="IWO89_001260"
     CDS             11010..13028
                     /locus_tag="IWO89_001260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HAQ0456028.1"
                     /translation="MENIARKERRRLEQTKRFRKAKRGAAIVGTAMVGCSVAAPLVQP
                     VPVQADETPVQFGARINTAAFITEIATYAQPIAQANDLYASVMIAQAVVESGWGSSAL
                     SQAPYYNLFGIKGSYQGQTVYMDTLEFLNNQWVNKKEPFRQYPSFAESFNDNAYVLRN
                     TSFGNGYYYAGTWKSNTKSYTDATACLTGRYATDPGYAGKLNNIIATYGLTQYDTPAS
                     GNAGGGATTGGNNTGSAGSTGGSSNSGNTNSGSATSTTYTVQSGDSVWGISNKFGITM
                     DQLIQWNNIQNNFIYPGQKLTIKGSQENGSSTNNSGNNTNSSGNAGTSNGGQTTGAKY
                     TVQSGDSVWKISNDHGITMAQLIEWNNIKNNFVYPGQQLIVSNGGSSNAGANTGSNTG
                     NTSGSSSSSSSSSNTASGTKYTVKAGDSVWSVSNKYGITMAQLIQWNNIQNNFIYPGQ
                     QLTVSNSGSTNSTSGNTGSSTNTNNSSSVNTGSSSSQGTYTVKAGESVWSVANKNGIT
                     MDQLIEWNNIKNNFIYPGQQLIVKGGTSANTNTGSTTSAAKPNTPNTSATTSTSSTGN
                     TMYTVKAGESVWSVANKHHITMDQLIEWNNIKNNFIYPGQQVIVKKGSSQSTNQQSVT
                     GSKTYTVKSGESVWSVADSHGITMAQLIEWNNIKNNFIYPGQTLIVAK"
     gene            13455..15869
                     /locus_tag="IWO89_001261"
     CDS             13455..15869
                     /locus_tag="IWO89_001261"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016183651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HAQ0456029.1"
                     /translation="MSYNHKEIEKKWQKYWAKTNTFNTHDDPEKPKFYALDMFPYPSG
                     QGLHVGHPEGYTATDILSRVKRSQGYNVLHPMGWDAFGLPAEQYALDTGNDPAEFTQK
                     NIETFRRQINSLGFSYDWNREVNTTDPEYYKWTQWIFTKLYEKGLAYEAEVAVNWVPE
                     LGTVISNEEVIDGKSERGGYDVVRKPMRQWMLKITAYADRLLDDLELVDWPESIKEMQ
                     RNWIGRSVGANIEFKVAGTDKSYTVFTTRPDTLFGATYSVLAPELDLVREITTPEQKA
                     AVEAYIEETAKKSDLKRTDLAKEKTGVFTGAYAINPVNGKEIPIWIADYVLSSYGTGA
                     IMAVPAHDERDFEFAQTFGLEILPVIEGGDVEKAAYTEDGTHINSEFLDGMNKQEAID
                     KMNEWLEENGVGKKEVSYRLRDWLFSRQRYWGEPIPIIHWEDGTVTAVPEEELPLRLP
                     KTKNIKPSGTGESPLANIEEWVNVVDPVTGKKGRRETNTMPQWAGSSWYYLRYIDPHN
                     KKELADYEKLKRWLPVDIYIGGAEHAVLHLLYARFWHKFLYDIGVVPTKEPFQKLYNQ
                     GMILGENNEKMSKSRGNVVNPDDVVETYGADTLRMYEMFMGPLDASIAWSENGLEGSR
                     KFLDRVWRLIVDENNKMRDRITTLNDGKLDKVYHQTVKKVTEDYENLHFNTAISQLMV
                     FINEAYKVDALPYEYIEGFVQLLAPIAPHMGEELWAILGNDGGISYAPWPTYDEAALV
                     EDEVEVVFQVNGKVRAKSNVPRDLGKDELEKAALANEIVQEYIEGKTVRKVIAVPNKL
                     VNIVAN"
     gene            16217..17770
                     /locus_tag="IWO89_001262"
     CDS             16217..17770
                     /locus_tag="IWO89_001262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase"
                     /protein_id="HAQ0456030.1"
                     /translation="MDQELFIIEVLSAKQKIIEKAKKRAKRIMFKLLSLVFFSGLLFF
                     GAVSFLYRHMSAVVRSAEGMQKLNNLYHFSWGVGGITIVVLIVFLLLIWLRYHQLSRH
                     FDQWLSQRAQQFLHNKPTSQDDDYLYFEKTSTSQAFRIQKSDCQLLLRSLDDLPIYLG
                     ESKSKFGMNTFHIFLLENEPRWEKSQSSPGGYLTTKMSTLVTGFFLILLAGTIYYGRY
                     TQPKMSSHINAASHMTMNSTNDTHIQKTNEGLLADPKQKVNLTTQKINDLKLDTETHS
                     LYMTTDSGANWVFVPIDSQWLRFGEYTLTTGLIPEGYWMDNTYDISADFSWFIYSSGD
                     DVRLLTSRDNGKTWQTSIITQSVQPIRYRKAVFYGNDGLFICTSASGMSAETLYIYTT
                     MDGGITWIKSGSTMINQPVQNVSFVTTSLGFVSTRESLFYTNNGGRSFKESVLSIPEN
                     YQTGGIDLFQSPNEVTQISANTLETKFYLLKKGPIDAGKMFACLFRSTDNGETWQFVQ
                     QLSQVEQGD"
     gene            complement(18151..19935)
                     /locus_tag="IWO89_001263"
     CDS             complement(18151..19935)
                     /locus_tag="IWO89_001263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ0456031.1"
                     /translation="MEKKKTLNTRALIRFWKMIKPEHKYFYGLFLCSLFGNLLVVAMP
                     LIMGIGIDDLLSEIRKTGIGQLSFSDIKQALLFPVLLLIGCSVLSSITSFIQERTMAA
                     LSEKITLRVRKEITKKFKTLPMSFFDNHQVGDIISRTTTGLNQLSQVLLTGINQFFTS
                     VVTIAAAGLMLFYIESKLTLLVLLLIVGSSIVTTKIANKNKQISEQNQYELGVLNNRM
                     EEILAGNLVVKTFNQQAHAEAAIHEVNKKQYNAFKRAQFMNCAIYPAIRFINQLAFII
                     SAVFGAMLVLSGGVTIGFLQAYLQYINQISEPISTASYVINSIQSAMAAIDRIFEILD
                     ETDEIPDLPDARLLEQPKGKIAFKNVQFGYTSDKILMNQVDFSVKPKQTVAIVGPTGA
                     GKTTLVNLLMRFYELNKGKIEFDGLDITKLSRGELRRSFGMVLQNTWLFEGTVADNIA
                     YGKMDATREQIIEAAEIAQCDHFIRTLPDGYDTIISSENGTLSQGQQQLLTIARVILA
                     NPPVVILDEATSSVDTRTEALIQQAMEAVTKDRTSFVIAHRLSTIENADMILVMKNGD
                     IVEKGTHSELLQSPTLYASLYNSQFQIA"
     gene            complement(19950..21671)
                     /locus_tag="IWO89_001264"
     CDS             complement(19950..21671)
                     /locus_tag="IWO89_001264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ0456032.1"
                     /translation="MSILKTFLQKNKGLALLTFVMICLEIAGTLGVPKLVAKLIDTGI
                     ASGEASVIKKIGVEMLLVAFLGTIAAVASSYFSALASARFGFQTREKYFRKFQQLSMK
                     DISRFSSGSLLTRMTNDVDNVQQMIVLFCQMILPAPVICVFTILMMFRYSLLLTWVTL
                     ISVVFYVWIVYRLMKRGAPLSLSIQPKMDRITITLREFFTGINMIRAFDNQSYEEKRT
                     NKSFADYANQMIKVNRIFAWVTPIAFLLMGIVYASILWFGGNLVAEGALQIGVVTAVV
                     EYSMLTLAYLMIAAMVLVIIPKSFASLRRIEEILQAEIEIEEETIKTVAEPTFDQTNA
                     AAFEHVTFKYTEISDPVLEDIDFTIPKGKTTAIVGGTGSGKSSLAKLLLRLSDPTIGK
                     VTLGGIPLTQMTQEQIRSHISYVPQKAFLFSGTILSNLQMGNEYASTEELSKAIKIAQ
                     LEELVDTLPDGLDSFVAQGGSNYSGGQKQRICIARALIKPAEIYVFDDSFSALDYKTD
                     AALRAALHKHMSEKTLVIVAQRLSTIQQADNIIVLDEGKIVGQGTHEELLSNCQTYQE
                     FAKSQGI"
     gene            21771..22532
                     /locus_tag="IWO89_001265"
     CDS             21771..22532
                     /locus_tag="IWO89_001265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HAQ0456033.1"
                     /translation="MKKMYTIGQVSELFDLPKSTIRYWDEQGLIRSSRQEENDYRLFD
                     IDDIFMLYDIVFYRKLDIPIKQMKNLYGKTLTELYETLDETERRIHKELVVMKQKQKE
                     IREQKKQLKLMIDTNEEEFPVEEIPFDCMISTEFEDIVEIKKFLPNYSSFGMMSMPAS
                     SSQTMYGFFIDPSEVHLFTQDVIWEKKDTAVYRRFLLKSEMNHAERNNILEIRERMYE
                     KGWKTGEVIGQYLLTNTDENNIRTEYYHAWIEMKK"
     gene            23499..23767
                     /locus_tag="IWO89_001266"
                     /pseudo
     CDS             23499..23767
                     /locus_tag="IWO89_001266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013720767.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS607 family transposase"
     gene            24439..>26615
                     /locus_tag="IWO89_001267"
     CDS             24439..>26615
                     /locus_tag="IWO89_001267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0456034.1"
                     /translation="MRNKFHKLLSMFLVMLIVINQFGNITVLAESLAEQPERSLTIEV
                     DQEEAALGEQFQLRVRGTPDLLNALVIDKSEDLIEGEVKQLNDYERLITYSSQHSGKY
                     ELTARSGDIQSNTVSIEISVPEESTSESFNVPEESTSESFNIPEESLSESFNEARSSE
                     PSITQESQIERQEELLNYDIGISAERPTLPSRHTQTLKGYIQGGAGDILEEYGEMVVR
                     VPRASIGLIEFIRVEVADDSPLKPLAFYQEGTDVVFPFTFDYEGLNEAAQSYTFSITF
                     EVAFFQSGGTEPNNDFQVSLTYAERAANTEFSITPIPSSRPIFSKLYYGSREIDSQGN
                     LGNLLIDGQDVTNSNKFILQVNYEGSRKDGVVVTDVLPQGTTLAPNPTTWSSTGNREP
                     ISGGVRIAEVTNPGEANVRTHYVSEEFVDKVSFNKETNTLVVDFGNLAEGQSYFIEYA
                     LEVTDVDLFNEGRQEDGFSRNRASMTYNNQVMSRDYRLRIPESNLPAISLRKSVDKNV
                     INLNENELTYTLHVSMFSGTRNTLVIEDVLEEGMEFVSFLSDIPSFIDAEVNNGIVRF
                     SANNTIGGPTSFTLSFKVNVEAYAVGDQILNFATLVNSDNRLNTSTVRTRKIDGRIKV
                     IKTDENGQRLAGAKFEILSSDSQVIQEGITAENGEFLSEPLPVGSYQVRETEAPEGYL
                     LDATVHDVFINEYSIEEVTLTIENILDTGGVELIKHAEGSGEVL"
BASE COUNT         8553 a         4659 c         5522 g         7881 t
ORIGIN      
        1 ctttttgagt atctttttct ttttatctga aagaatgatt ttaaaaagct tagcagagtt
       61 atctttatgg atgattgtgc taagcttttt tggacagttc taatgtaaaa tatttttata
      121 ttttttaagc aaaaaaatga aaaaatacga gcagaaaact gttttatctg ctatacttgg
      181 ttcgttgcaa gttcccgaaa ggatccgcag gacggagatg ccttgtaacc gaatttaact
      241 agggggaatc attataatgg tagaaagaca cttatttaca tcagaatctg tttctgaagg
      301 acatccagat aaaattgctg accaaatcag tgatgcaatc ttggatgcaa ttttaaaaca
      361 agatccaaca gcacgagtag cgtgtgaaac gtctgtaaca actggtcttg ttttagtatt
      421 tggagaaata tctacgacgg catatgtaga tattcaaaaa gtcgtacggg aaacaataaa
      481 agaaattggt tatacacgtg caaaattcgg atttgatgga gatacagcag ctgtattggt
      541 tgcgattgat gaacaatctc ctgatattgc tcaaggagtt gatgaagcac ttgagatccg
      601 ggatgaagat aaaaaagacg tattagatga gattggtgct ggtgaccaag gtttaatgtt
      661 tgggtttgct gtagatgaaa caccagaatt gatgccttta ccaatcgctt tgagtcatcg
      721 tctggtacga cgcttggcag atttgcgtaa atcaaatgaa ttaacgtatt tacgtccaga
      781 tgcaaaatct caagtaacgg ttgaatatga tgatcaagga caaccggaac gcgtagatac
      841 aatcgttatt tcaacacagc atgatgatgc agtagataat gaaacaattc gtcatgatgt
      901 catcgaaaaa gtagtgaagg aagttattcc agctgaatta ttagatgatc aaacgaaata
      961 ttatatcaat ccgactggcc gatttgtcat tggtggtcct caaggggatg ccggattaac
     1021 aggaagaaaa atcattgttg atacgtatgg cggttatgct cgtcatggtg gcggtgcttt
     1081 ttctggtaaa gatgcgacaa aagttgaccg ttctgccagc tatgctgcac gttatattgc
     1141 taaaaacatt gttgcagcag ggcttgctcg caaagcagaa gtacagctag cttatgcgat
     1201 tggtgttgct caacctgttt cgatctcaat caatactttc ggtactggaa cggttccgga
     1261 agaagaactg attgctgcag tgagagaaaa cttcgatctt cggccagcag gaatcatcga
     1321 gatgcttgat ttgcgtcgtc cgatttacaa acaaacagct gcttacggtc attttggtcg
     1381 tacagatgta gatttgcctt gggaacatac ggacaaagta gatgcgttga aagcaagttt
     1441 agcaaaataa gttatattca gctggaacga tcgccaagtg gtggttgttc cagctttttt
     1501 tatggcagtt aacctcttga aaaagatagt aattatttat ctatttttag atcctgtcat
     1561 aatgaaaggg aggaataaga tgaaaaaaga aacaaatgtt actttggtca cagtgagtat
     1621 tttcattgca acgttcatga cggctattga aggtacaatc gtaacaaccg ctatgccaac
     1681 gattgtcggt tcattacatg gaatggaaat catgaactgg gtattttcga tttatttgct
     1741 gacaaacgct atgcttactc cgatttacgg aaaacttgcg gataaagtgg gacggaaacc
     1801 aatctttatt tttgggacat tattgtttat tatcggttcg ttattttgcg gtttatctca
     1861 aagcatgatt gggttgattg ctgctcgtgc gattcaaggg atgggtgctg gggcaatgat
     1921 gccagtagct ttgacgatca tcggcgatct ttattctatt gaaaagcgag caaaagtttt
     1981 aggtttgaat agttcagcct gggggattgc cagtgttttt ggaccgcttg cgggaggatt
     2041 catcgttgaa acgatcagct ggcactggat cttttttatc aatgtgccga tcggaattct
     2101 attaattatt ttgatttggc tatatttaaa tgaaccaaaa atcatacatg agtctaaacc
     2161 aatggatatt tttggtagtt ttagtttgat ggccatgctt gtctcattgc tgttagggtt
     2221 ccaattatta ggagacgaag gaatgagtcc gcttgtttct gctttgctgc taggtagtat
     2281 cgtaatgtta ggtatttttg tttttgccga aaaaagagct aaagatcctg tcatcatgat
     2341 gcatttattc aaaaatccac tatttgttgt ggtcaacttg gctgcagcat tggctagtgg
     2401 ttttttgatg ggaatcgatg tttatatccc tatgtggatg caaggtgtat tagggctgaa
     2461 tgcagcaatt ggtgggctgg tattagcacc attatcacta gtctggatgg tcggttcttt
     2521 ccttgcaagc aagtggatga ctcaaatgaa aactcattgg gtcttgagaa ttggactact
     2581 aatcgcagct attgggggcg cttggttgta tcttatgcct ttcagaagta actatttatg
     2641 gttttttgct atatcaacgg tcatcggtat aggcttaggt gtgaccatga ctacctcgac
     2701 tgtcagtgca caaaatagtg tatctcgtga ccagcttggt gtagcaactt cttttaatac
     2761 gttagttcga acaattggtc aaacagtcat ggtttctatt tttggtttat tgataaatgg
     2821 ggtaactaaa cgagaacttg cagcagcaaa tttaacaaat gatgcagata tcatgaataa
     2881 actggtgaat ccacaaacgg ctaaatatat tgatgcggat attttgttac cattaagaaa
     2941 aattctttat gatgggttac attccgttta tctagttggc ttgttactcg tgatcattgc
     3001 tttggtctta acgcttttcg ttcatgaaaa aagtggtaaa ataaaacagt agtttcggtt
     3061 aagatatgta cgtagccgaa tcattcaaat ttaaagttat tcaaaaataa cttcttacta
     3121 aggaaggcga aaatatggca tttattcaag caaatattta ttcgaatgtg ttagaaatgg
     3181 aagtcaatct aaatgtgata ctaccgcaaa caacaaaaaa ggtgattggc accagttcag
     3241 agcaagagac aacggatata cctgttttat atttacttca tggaatgggt ggcaatcata
     3301 gtgtatggga aagacgtacg tcgattgaac gttatgtagc aaactacgga ttagctgtca
     3361 tcatgccatc tactgatctt ggctggtaca cagatacagt ttatgacatg aagtattgga
     3421 cattcattgc agaagagcta cctgagattt gtcatgaatt attcccgcaa ttaacaagaa
     3481 aaagagaaaa aacatatgcc gctggattat cgatgggcgg atatggcgcg ttaaaactag
     3541 gccttgcaaa atcagaaaat ttcgcagcag taatctctct ttcaggcgct gtttctgtag
     3601 gaaatcgaat ggatgatctt ttgatggttc gtaaagcatc tttttgggaa gggatctttg
     3661 gaccattgga tcaaatcgaa ggttcaaaaa atgatccgca atacttattg aaagaactag
     3721 taaaaagcgg aaaaccagtt ccaagatttt atatggcttg tggggaatct gattttcttt
     3781 atcaggcaaa tcaggaaatg gtatctgcta tgcaggaaca aggcttggcg gtcacctttg
     3841 aacaaggacc tggcgatcat aactggatct tctgggacca atggattcaa cgtgcattgg
     3901 cgtggctaat ggaagaaaaa taaaaaaaga gtcttaaaaa gatagctcct atagagatca
     3961 caaacatagt gatctctata ggagcttttt tactgctcgt tttctcttct tttattttgt
     4021 tggcataagc cttcaatata ggtttgtgca ctcgttagaa agagtgcttt ttcattcaat
     4081 tctgcatggg actgcatact ttctttaaat ccctctaatt gattttcaac tacataatta
     4141 cgcataaata gaactgtttc tttcaaaaat tcatttccgt ccataatttt ttgtcgaagc
     4201 tgctgttcat gctgctgatc cattaactgt ccaatcaata aattgtggag agaggcaatg
     4261 gcctgatgac tttgggtgac tgaaaatcct tcacgaacaa gtaattggat catttgctcc
     4321 aaatgttcta atcgactcgg aaaagcgggc acagtattga tttctaattc tgccccacaa
     4381 gggaaacgtg tataaaggtc atagtaattt tccatatact gcagcaactg ttcattccaa
     4441 ggcaaagttg gatctggaag cgctaattct tcttccattg tttcagccat tatttgcaag
     4501 agctgttgtt tgtttttgaa ataccaatat aaagcaggtg cttgaacatg aattctgttt
     4561 gcgatgtttc gcatggaaag ctgcgaaaga gcttgtgttt cagccaggat atcaaaggcg
     4621 gcctgaatga tgattgcttg agaaagcttt atttccattt tatcaacctc gtgtattgca
     4681 ttatcttttt aaacatggta aattgttagt cggtctaatt ttacagtgtt aaattagtcc
     4741 tgtcaattta aacaaagggg tgtaatcaac gaatgaaatt aatcttacga catgcgaaaa
     4801 aatataaagt agctgttttt atttccttgc tgtccgtagc agtcatggtt gcagcagcat
     4861 tatggcaacc taagttacta cagcaagtat tggaagctat tattacagaa gataatgatg
     4921 aaatgaaaac aattggtatt tacctgatca gtctggcttt gctaggatta gccgcaggtg
     4981 taacgaacac gattttctct gcaaaagtag ctcaaggcgt aagtgcagat atccgagaag
     5041 aagcatttcg aaagatccag acattttcat ttggaaatat cgaacagttc tctgctggaa
     5101 atcttgttgt tcgtctaaca aatgatatca cgcaaatcca aaatttagtg atgatttctt
     5161 tgcaatcact ttttcgaatt ccattcttat ttattggtgc cttcatttta gctatgatca
     5221 cgatgccgca attatggtgg atcatcgtag cgcttatcgt tacggtattt ctgattacgg
     5281 cactttcatt tacaaggatg ggcaagcact tcatgatcat tcaaaaactg attgatcgtg
     5341 tgaatagcat tgcaaaagaa aacttactag ggattcgtgt agtgaaatct tttgtacaag
     5401 aagaaaacca attgaagtca ttcagcaaag taagtgagga cttaacaaaa cacaacatca
     5461 tagtcggtac attattttct gtgatgatcc cgtccttcat gctggctgcc aatttggctg
     5521 tggttggggc gatctttttt gtgagcgatc ttgttaaaga tgatcctacg ttaattggtg
     5581 gaattgcttc attcatgaac tacttgatgc agattatgat ggcaatcatt attggcggaa
     5641 tgatgatgat gatgacttca agagcagccg tatcgctgaa gcggattgcg gaaattttag
     5701 atgctgaacc agatcttact tatttagacg ttccagaaca agagttgacg ggttctgtca
     5761 cttttgacca tgtttctttt cgttatcctg gtgaggatac agatacgctt aaagatatca
     5821 gcttttctat caaacctgga gaaatggttg gtatcgtcgg ggcgactggt gccgggaagt
     5881 caacactagc acaattgatc cctcgtctat ttgatccgac agaaggcaaa gttgaagtag
     5941 gtggtgtaga tcttcgtcaa gtaaatgaaa agagtttaag acaaacggtt tcctttgttt
     6001 tgcaaaaagc aattttattc tcaggaacga ttgctcaaaa cttgcgccaa gggaaaaaga
     6061 atgcaacaga aaaagagatg gaacatgctt cttctattgc tcaagcaaaa gaattcatcg
     6121 aaaagttatc tgatcgttac gaagcaccaa tagaagaacg tagcagtaac ttttctggtg
     6181 gtcaaaagca acgtttatcc attactcgtg gcgttatcgg taatccaaag atcttgatcc
     6241 tagatgacag tacgagtgca ctggatgctc gctctgaaaa gctggtccga gaagcactcg
     6301 atcgagattt gaagggaaca acgacgattg tcatcgcaca aaaaattgct tctgttgtca
     6361 aagcagaccg catcttagta ttggatgaag gcagattagt cggcgaagga acccatgagg
     6421 aattggtagc tggaaaccgt gtctatcagg aaatctttga aactcaaaaa ggaacggagg
     6481 agtaagtatg actgatctaa taaaagcaag taaatttttc tatcactatt tgaagcggta
     6541 caaactctcc tttttcttta tctttatcac agtgatcatt gctacctatc tacaagtaaa
     6601 ggcacctcaa tacatcgggg aagcttttca agaattagct aattacgtag gaggcttgat
     6661 gcaaggcgta gacgataaaa gcaaatttgt ttctgtaata tggaaactct tgctgtttta
     6721 tgtactagct agtgcagcca actttatcta tagtattttg ttcacacaag tagtagggaa
     6781 gtcaacgaac cgaatgcgta tcggtctatt caacaaattg gaaaaattga cgatccgttt
     6841 ctttgattct catcaagacg gtgaaatttt aagtcgtttt accagtgact tagataatat
     6901 tcaaaatagt ttgaaccaag ctttgcttca agtcatcacg aatgctgttt tacttgtcgg
     6961 gatcttgatc atgatgttcc gccaaaatgt agagttagct tgggctacaa ttgcttccac
     7021 tccagtagcc gttttgatag ctgtcttgat tatccgcaaa gcacggaaat atgtggatct
     7081 tcagcaagat gaagtgggga aactaaatgg ttatatggat gaaaaaatca gcggacaacg
     7141 tgtcatcatc acaaatggct tacaagaaga aacgattgaa ggattcttag agcataatca
     7201 aagtgttcgc gaagcaacct tcaaaggaca agcttattct ggtctactat ttccgatgat
     7261 gcaaggaatg tcactggtca atacagcgat agttatcttt ttcggcggct ggttagcatt
     7321 aaatggtgat ttggaacgtt cggtggcatt aggcttagtt gtgacattcg tccagtattc
     7381 acaacaatat taccaaccac tgatgcagat ttcatctgga tacagcatga tccaattagc
     7441 gattacaggg gcaagacgat tgaatgaaat atttgatgaa aaagatgaag tcaaaccaga
     7501 aaactgtaaa gcatttgatg gtgttgaaaa aggtcttgcg ttgcagcacg tagattttgg
     7561 ttattctcca gaaaaactag tcttgaaaga tgtctcgatc aatgtggaca aaggagaaat
     7621 ggttgctctt gttggtccaa caggatcggg gaaaacgacg atcatgaatc tgctaaaccg
     7681 tttttacgat gtcaatgatg gttcagtagt catcgatgga atagatatcc gagattatga
     7741 tcttgatgca ttacgtagac atgtaggaat cgttttgcaa gaatctgtat tgttctccgg
     7801 aacgatcaaa gacaatatca cattcggcaa accggacgct tcagatgaag aagtcatcaa
     7861 tgcagcaaaa caagcgaata ttcatgactt catcatgacg ttagaaaaag gttatgatac
     7921 agagatatcc gaagaaaata acttattcag tacaggtcaa aagcagttga tcagtattgc
     7981 tcgaacgatc atcacaaatc catctttatt gattttggat gaagcaacaa gtaatgtaga
     8041 cacggtaaca gaagcaagga tccaaaaagc aatggatgaa gcaatcaaag gaagaaccag
     8101 ctttgtgatt gctcaccggc tgaaaacaat cttgaatgca gatagaatcg ttgtcttgcg
     8161 tgatggggaa gtcattgaag aaggcagtca tcacgagtta ttagcagaaa atggatttta
     8221 tgcggaactt tatcataatc aatttgtatt tgaataaaga ggttgtgaaa aagctgtcct
     8281 gcatcaagta ataagaacag ccaatcaaaa acagcttctc atgtttttga tttggttgga
     8341 cttattaccg cagatataag cttttgagca ccgtgaataa agaggttatg aaatcaacgc
     8401 tctaacctga gtattagaga aaaaaacgca aaacatatca tttatgtttt gcgtttttta
     8461 tttatctttt tttctcaata caatataaaa taggcggctg gttcttttgg ttgataaacc
     8521 gataattcag cacattatat gtttcctgtg gcagtttgga acaaaaagaa tcaaccatat
     8581 ccagttcttt ttctccacct tcatgaccat agtagacgac aaggatcaat cgtccgcgag
     8641 gcacaagtcg tttcaatatt tcttccatag ctgtgcgtgt agtctctggc atagtgatga
     8701 ttgctttatc actttttggg agatacccta gattgaaaat ccctgctttg agattttgtt
     8761 gttcagctat cacttctcct aacgtttcgt ggccttgtag aaacaatgag acacgttctt
     8821 ctaactgacg ctgttctagc ctttcacgag tagcatcgat tgcttgttgc tggatatcaa
     8881 aactatagac atgaccagtt tttccgatgt gttctgctag aaaaaccgta tcgtggccat
     8941 tgcccatcgt tgcatctacg acgtgatccc ctggttgcaa gatttcttgc agtaatgtat
     9001 ggctgaaatg caaagcagtt tgcaacatca gacgctcacc tttttccgta catcatattt
     9061 tccttggaag ctatctctgc gaagaagttc ttcatcaatt gcattcaaga cttcccattt
     9121 tttcaagctc cacattggtc caatcaagga atcccacggc gcatcccctg tcaaacgatg
     9181 gatgatgatc tcttttggta tcatttccag ctggtcgcaa atgacattga catagtcagg
     9241 acggctcatt aattgaaggc gaccttcatg atagtcccgc aacattctcg ttttgcgcat
     9301 cagatgcatc aaatggagct tgattccttg gatatctgaa tctaaaatgg tgcgtctgac
     9361 attttctttc atcatttcta aactttcgcc tggtaatccg ttgatcaaat gcgtacaaac
     9421 acggatatta tgtttgcgta atttagccac gccatctaaa taagtttgat aatcatgtgc
     9481 tcggttgatc agttttgaag tttcttcaaa agttgtttgc aatccaagtt ctacccataa
     9541 atacatccgc tcgttcagtt cagcaaggta atcaactact tcatctggca gacaatccgg
     9601 tcgtgtaccg actgataatc ctacgacacc tggtaaattg actacttgct caaatcgttc
     9661 tttgattatt tcaactggcg catgggtgtt tgtaaaattt tggaaataaa cgatgtattg
     9721 ttggacatga ggccattttt gatgcatcat gtcgatttct ttctggaatt ggattggaag
     9781 agggtcttca ggtgcaacga tcatgtctcc cgaaccagat acactgcaaa aagtacaacc
     9841 gccatgggca acagtcccat ctcgattcgg acagtcaaat cctccatcga tcggcacctt
     9901 gaaaattttc ccgccaaact catttcgtaa agcgtaattc caagaatgat atcttttatt
     9961 tccttctgaa taaggaaaag tcattaaaag tccttctttc tgtaagagtt atctatttgt
    10021 tttctttttt ctctatacac tggataagtc aggcacagcc aagaaagact gagtaagtaa
    10081 ccgccgatga tatcagtagg aaaatgaacg cccagataaa tgcggcttat cccgacactt
    10141 actataataa acatcatcag caattgaagc aatgtgcgta aggttctttt ttgaacaaaa
    10201 gctggaagca gaaatagtaa agaaccgtaa agaatcatac tgcctgctgc atgtccacta
    10261 gggaaactgt aacttgtttc aactactaag tgcgccaatg aaggacgctc tcgcatgaaa
    10321 aatagtttga tcaccgggtt gatgattcca gcaattacaa taaatccaga aacgaaccaa
    10381 aatgtttctg cccttttttt cttataccac atccataaag ccgcacaagc aaataaaatg
    10441 atgacggtaa gaggattacc gaatttagtc ataaagagtt ggtagctgtt cagatcagga
    10501 taagaagctc tgacaatgcc tgtaaaccac tgatcgaaac cagaaagcca cgacagatgg
    10561 aactttacga tccagcccaa aatgaaaaac aggacaagaa agaagctacc agccacctga
    10621 aatgatgttt tatatttcat acaattcctc ctttgaagca atgacttatt atagcatttt
    10681 tggtccagcg aaaaaaacgg taaagagata aaaccaatgt atgattcctc tttgaataac
    10741 caatattctt ataaaaaaaa taactgtaat cggaaagaca ttttttggta atgcaaatga
    10801 aatgttaggg aaataaaatc agtgtttttc gaaatccttt taaacaagaa cgtagacgta
    10861 ggaagaactt gaaaatgaac agaagttgtt cattacggta aattacaata tgattacaaa
    10921 aaacaacaaa gctcttaaat aacttttaat aaatattaag agaagttata attattaata
    10981 atagataaat tgaagttgga ggatagaaga tggaaaacat tgcaagaaaa gaacggagac
    11041 gtttagaaca gaccaaacgt tttcgaaaag caaaacgagg ggcagcaatc gtgggaactg
    11101 cgatggtagg ctgttctgtc gctgccccat tagtacagcc ggttcctgtt caagcggatg
    11161 aaacacctgt tcagttcggc gcaagaatca atacagcggc atttatcaca gaaattgcta
    11221 cttatgctca accaatcgct caagccaacg atctatatgc atcagtgatg atcgcacaag
    11281 cagttgtaga aagcggttgg ggcagtagtg cgttatctca agcaccttat tataatcttt
    11341 ttgggataaa aggcagctat caaggacaaa ccgtctacat ggatacgctt gaatttttaa
    11401 ataatcaatg ggtaaataaa aaggagcctt tccgacaata tccttctttt gctgaatctt
    11461 tcaatgacaa tgcctatgta cttagaaata cttcatttgg caacggttat tactatgccg
    11521 gtacttggaa gagcaacacg aaatcctata cagatgcgac agcttgtttg acagggcgtt
    11581 atgcgactga tcctggttac gcaggcaaat taaataatat catcgcaact tacggattga
    11641 cccaatatga tacaccagct agcggaaacg caggcggtgg agcgactaca ggtggcaata
    11701 atactggaag tgcaggtagt actggcggtt caagcaattc tggtaataca aactcaggtt
    11761 cagcaacttc gaccacttat actgtccaat ctggtgattc tgtttgggga atctcaaata
    11821 aattcggtat cacaatggat caattgatcc aatggaacaa catccaaaac aatttcattt
    11881 atccaggaca aaaactgaca atcaaaggta gccaggaaaa tggctcttcg acgaataatt
    11941 ccggtaacaa taccaattct tcaggaaatg ctggtacatc aaacggagga caaacaactg
    12001 gtgcgaaata cactgttcaa tctggtgatt ctgtttggaa gatctcaaat gatcacggaa
    12061 ttacgatggc tcaattgatt gaatggaaca atatcaagaa caatttcgtt tatccaggac
    12121 aacagctaat cgtatcaaat ggcggttctt ctaatgctgg agcaaatact ggctcaaata
    12181 ctgggaatac aagtggctct agttcgtcca gctcaagttc ttctaataca gcttcaggaa
    12241 cgaaatatac agttaaagcc ggagactctg tttggagcgt ctcaaataaa tacggaatta
    12301 cgatggctca gctaatccaa tggaacaata tccaaaataa ctttatctac ccaggacaac
    12361 aactgactgt ttcaaacagt ggatcaacaa acagcacgtc aggtaataca ggttcgtcaa
    12421 ccaatacaaa taacagcagt tcagttaata ctggcagcag ttcgtctcaa ggcacttata
    12481 ccgtgaaagc aggtgaatct gtttggagcg tggcaaataa aaacggcatc acgatggatc
    12541 agttaatcga atggaataat atcaaaaaca acttcattta tccaggacag caattgatcg
    12601 ttaaaggtgg aacttcggca aatacaaata caggttcaac tacttctgca gcgaaaccaa
    12661 atacaccgaa tacgtctgct acgacaagta catcatccac tggtaatacg atgtataccg
    12721 tgaaagcggg cgagtcagtt tggagcgtag cgaacaaaca tcatatcacg atggatcagt
    12781 taatcgaatg gaataatatc aaaaataatt tcatctatcc aggacagcaa gtgattgtga
    12841 agaaaggttc atctcaatca acaaaccaac aatcagttac tggttcgaaa acctatactg
    12901 tgaaatctgg agaatctgtt tggagtgttg ctgacagtca tggaattaca atggctcaac
    12961 taatcgaatg gaacaatatt aagaacaact ttatttatcc aggtcaaacg ttgattgtag
    13021 ctaaataaaa tagctgttcg aagaaataac gattgaattc cacaaaaata tttgttacta
    13081 taaggctact taaatatgac aacacttgaa aaggaagtag taaaaagact gaagtttcag
    13141 agagcgcatg gctggtgaga atgtgcacgg atgcttttga actcgccttt gagttgccct
    13201 tgctgaatac ccgagtaagc aagcggcggt gacggccgtt acaacgttcg aggccttttc
    13261 actaaggtaa aattaggtgg taccgcgtag attacgtcct ataaaaagag caatcttttt
    13321 ataggatttt tttatttctt ttgtgaagtt tgagaaaaca aagcaaaagg aaataaaaat
    13381 aaacagattc tgccggtttt ttcgttatat ttataataga aacaagtgtt ttcaatagaa
    13441 caggaggaca gcaaatgagt tacaatcata aagaaattga gaaaaaatgg caaaaatact
    13501 gggcaaaaac gaatactttc aacacccatg atgatcctga aaagccaaaa ttttacgcac
    13561 tggatatgtt tccctatcca tccggtcaag ggctacatgt agggcatcct gaaggttata
    13621 cggcaacgga tatcctttca cgtgtgaaaa gaagtcaagg atacaacgtg ctgcatccaa
    13681 tggggtggga tgcattcggt ctgcctgctg aacaatatgc gctagataca ggaaacgatc
    13741 cagctgagtt tacccaaaaa aatatcgaaa cattccgacg ccaaatcaat tctttaggat
    13801 ttagttatga ctggaatcgc gaagtgaata caactgatcc ggagtattat aaatggacac
    13861 aatggatctt tacgaagctt tatgaaaaag gtctagctta tgaagcagaa gtagcggtaa
    13921 actgggtacc tgaattagga acagttattt caaatgaaga agtgatcgat gggaaaagtg
    13981 aacgtggtgg ctatgatgta gtcagaaaac caatgcgcca atggatgctg aaaatcactg
    14041 cttatgctga tcgcttattg gatgatctag aattagttga ttggccagaa agcattaaag
    14101 aaatgcaaag aaattggatc ggtcgttcag taggagcaaa tattgaattt aaagtggctg
    14161 gtacggataa atcctatacc gtatttacga cacgaccaga tactctattc ggtgcaactt
    14221 actcggttct tgcacctgaa ctagatcttg ttagagaaat tacgacccct gaacaaaaag
    14281 ctgctgtaga agcttatatc gaagagacag ctaaaaaatc agatctaaag agaacggatc
    14341 ttgcaaaaga aaaaacagga gtatttactg gtgcttatgc aatcaatcca gttaatggca
    14401 aggaaatccc aatctggatc gccgattatg tattatcttc atacggcaca ggagccatca
    14461 tggctgttcc agcacatgat gaacgtgact ttgaattcgc tcaaactttc ggactagaaa
    14521 ttcttccagt tatcgaaggt ggagatgttg aaaaagctgc ttacacagaa gatggtacgc
    14581 acatcaactc tgaatttcta gacggcatga acaaacaaga agcaatcgat aaaatgaatg
    14641 aatggctaga agaaaatggc gtagggaaaa aagaagtcag ctatcgctta cgtgactggc
    14701 tattctcccg tcaacgttac tggggtgaac caatcccaat catccattgg gaggacggaa
    14761 cagtcacagc tgtcccagaa gaagagctac cattacggtt gcctaagaca aaaaacatca
    14821 agccaagcgg tactggagaa tctccattag caaatatcga ggagtgggtc aatgtcgttg
    14881 atcctgtaac aggtaaaaaa ggccgtcgcg aaacgaatac aatgccacaa tgggcaggaa
    14941 gctcatggta ttacctgcgt tatatcgacc ctcataacaa aaaagaacta gcagattatg
    15001 aaaagttgaa gcgttggcta ccagttgaca tctacattgg tggagcagaa catgcggtac
    15061 ttcatttact atatgcacga ttctggcata aattcttata cgatatcggt gttgttccta
    15121 caaaagaacc attccaaaaa ctatacaatc aaggaatgat cttaggcgaa aacaacgaaa
    15181 aaatgtcaaa atctcgtggt aatgtagtca atcctgatga tgtggtcgaa acttacggtg
    15241 cagatacact gcgcatgtac gaaatgttca tggggccatt agatgcttcg attgcttgga
    15301 gcgaaaatgg cttagaagga agccgtaaat tcttggatcg tgtatggcgt ttgatcgttg
    15361 atgagaacaa taaaatgcgt gaccgtatca ctacattgaa tgatggcaag cttgataaag
    15421 tgtatcatca aacagtgaaa aaagtgacag aagactatga aaatcttcat ttcaatacag
    15481 caatctccca attgatggtc tttatcaatg aagcctataa agtggacgct ctgccttatg
    15541 aatatatcga aggatttgtt caactactcg caccgattgc tcctcacatg ggtgaagaac
    15601 tatgggcaat ccttggaaat gatggtggta tttcttacgc accatggcca acatacgatg
    15661 aagcagcact tgttgaagac gaagtagaag ttgttttcca agtaaacggt aaagttcgtg
    15721 cgaaaagcaa tgttccccga gacttaggaa aagatgagct agaaaaagca gcattagcca
    15781 atgaaatagt gcaagaatat attgaaggca aaacagtcag aaaagttatt gcggtaccaa
    15841 acaagttagt caatatcgtt gctaattaat tggctctttg tcaataagga ctgatgagtt
    15901 gtgcaaaatc aaatctgggc cagaatgaac ttcattctgt ctcagatttt ttgttatttc
    15961 aaattgatta attgattgat caaaaaggtt ttgattttca tgttctcaaa cacgttcaat
    16021 gtctttttct tctgaatggt cttgtaagag atctctgtaa tcatcaaatt tttgtctatt
    16081 aatcttagta ttttttgatg tataaactac ctgaaattga gagtacatac caaattcata
    16141 aattttttat tagtgggact agtgattgta tggattttga cgcataatga atgaagtatg
    16201 agaaaaggag agacaaatgg atcaagaact atttattatc gaagtattat cagctaaaca
    16261 aaaaattatc gagaaagcga aaaaaagagc gaaaagaata atgttcaagc ttttaagctt
    16321 agtatttttc tctggtttgc tttttttcgg agctgtatcg ttcctatatc gccatatgag
    16381 tgcagtagtc cgttcagctg aagggatgca aaaattaaat aatttgtatc acttttcgtg
    16441 gggagttggc ggaataacaa ttgttgtgct tattgttttt cttctactta tttggctccg
    16501 ttaccatcaa ttgtcgcgtc atttcgatca atggcttagt caaagagcac aacagttttt
    16561 acataataaa ccaacttctc aagatgatga ctatctttat tttgagaaga cttcgacgag
    16621 tcaagctttt cggatacaaa aaagtgattg ccagttattg ctgagatcac ttgatgattt
    16681 acctatctac ttaggcgaaa gcaaatcaaa attcggaatg aacacattcc atattttttt
    16741 actagaaaac gaacccagat gggaaaaaag ccagtcttcg cctggtggat acctgacaac
    16801 taagatgagt acattagtca ctggtttttt cctcattctt ttagctggaa caatctatta
    16861 tgggagatac actcagccaa aaatgtcttc tcatataaat gctgcttctc acatgacaat
    16921 gaactcaact aacgatacgc atatacaaaa gacaaatgaa gggttgttag ctgatcctaa
    16981 gcaaaaggta aatttgacaa cacaaaagat aaatgatctc aaacttgata cagagacgca
    17041 ctctctctat atgacaacag atagtggtgc taattgggta tttgtgccga ttgattcgca
    17101 atggttgcgt tttggcgaat acaccctgac aacggggctg attcctgaag gttattggat
    17161 ggacaacaca tatgatattt ccgccgattt ctcatggttt atctattctt ctggggatga
    17221 tgtacgtctg ctgacttcaa gagataatgg taaaacatgg caaacgtcta ttatcaccca
    17281 atcagttcag ccgatacgtt atcgaaaagc ggtattttat ggcaacgatg gtctatttat
    17341 ctgtacatca gccagtggga tgtctgcaga aactttgtac atttacacta ctatggatgg
    17401 cggaatcact tggataaaaa gtggaagtac aatgatcaat cagccagtac aaaatgtcag
    17461 cttcgttaca acttctttag gttttgtttc gacacgtgaa agtttgtttt atacaaacaa
    17521 cggaggacgc tcattcaaag aatcagttct gtcgattccg gaaaactatc agacaggggg
    17581 gatagatctt tttcaatcac caaatgaagt tactcaaata agcgcgaata cgttagagac
    17641 aaaattttat ttgttgaaaa aaggaccaat cgatgcaggg aaaatgtttg cctgcctctt
    17701 ccgttcaact gataacgggg aaacatggca attcgttcag caactatcgc aagtagaaca
    17761 aggagattaa agaagagaga ttgtgagcct gacgttcagc accgagtcac aagaaacaaa
    17821 ttcaaaaaac agcttctcat atttgttgaa tagtgtaact cgtgattcgg agttgacaag
    17881 agcaacaaga agcgattttg aagtatagct tctcgtattt tacaagatcg tgtcttattg
    17941 ccgataggtt gtctcttgaa caccgtttat tcgtaaaaag ggattgtgac aagtcttttg
    18001 tcactactta ccgaacgaaa ctgaaggagc tatccaaaaa gtatcattag tattattctt
    18061 tgtgtttcta gtggcaccac tagtacattt aaaaaagcta tcgatcatgg actaatcata
    18121 catgatcgat agctttttag ataatgtttg ttacgctatc tgaaattgac tattataaag
    18181 actggcataa agcgttggtg attggagtag ttcagaatgt gttccttttt cgacgatatc
    18241 gccattcttc attacaagga tcatgtctgc attttcaatc gttgacaaac ggtgtgcaat
    18301 cacaaaactt gtcctatctt ttgtcacagc ttccattgct tgctgaatca atgcttctgt
    18361 tcgtgtatct acgctagaag ttgcttcatc taaaataacg acaggaggat tcgctagaat
    18421 gactcgagca attgtcaata attgttgctg tccttgagat aacgtgccgt tttcgctaga
    18481 aatgatcgta tcatatccgt ctggcagtgt tcgaataaag tgatcacatt gtgcgatttc
    18541 agccgcttcg ataatttgtt ctcttgtggc atccattttc ccgtaggcga tattatcagc
    18601 taccgttcct tcaaacagcc aggtattttg taaaaccatc ccaaaagaac gacgtagttc
    18661 tccacgtgaa agcttggtaa tatcaagacc gtcgaattcg atctttcctt tattcagttc
    18721 ataaaaacgc atcagcagat tgactaaagt cgtttttcct gcaccagttg gaccaacgat
    18781 cgctaccgtt tgttttggct ttactgaaaa atcgacttga ttcatcaata ttttatctga
    18841 tgtgtaacca aactgtacat ttttgaaagc tatttttcct ttcggttgtt ctaaaagtct
    18901 tgcatctgga aggtctggaa tttcatctgt ttcatctaat atctcaaata tcctatcgat
    18961 tgctgccatt gcggattgga tcgagttgat cacataagaa gcggtagaaa ttggttcaga
    19021 aatctgattg atgtactgaa gataagcttg taagaagccg atcgtcacac cacctgatag
    19081 aacaagcata gcaccgaata ccgcacttat gatgaaggcg agttgattaa tgaaacgaat
    19141 ggccggatag atagcacagt tcataaattg agcacgttta aaagcgttgt attgcttttt
    19201 atttacttcg tgtatagcag cttctgcatg agcttgctga ttgaatgttt tgaccactag
    19261 attaccagct aagatttctt ccattcgatt atttaaaaca cccagttcat actgattctg
    19321 ttcagaaatc tgtttgtttt tgttcgcaat cttagttgta acaattgaac tgccaacgat
    19381 cagcaataag acgagcaaag taagcttgct ttcgatataa aacagcatca gaccagcagc
    19441 agctattgtt acgacagaag taaaaaattg attgatacct gtcaataaca cttgagaaag
    19501 ttgattgaga ccggtagttg tccgactaat gatgtcacct acttgatgat tgtcaaaaaa
    19561 cgacatcggt aatgttttga atttttttgt gatttctttt cgcaccctca atgtgatctt
    19621 ttcgcttaac gctgccattg tccgttcctg aataaatgaa gtgatgctgc ttaagactga
    19681 acagccgatc agcaataaga cagggaacaa caatgcttgt ttgatatcac tgaaagaaag
    19741 ctgtccaata cctgtttttc ttatttctga taagagatca tcgatcccga ttcccatgat
    19801 caagggcatg gctacgacta ataggttccc gaataaactg catagaaaca atccgtaaaa
    19861 atatttatgt tcaggtttga tcattttcca aaaacgtatc aaagcacgcg tgttcaacgt
    19921 ttttttcttt tccatcgtat cccttctttc tatattcctt gtgattttgc aaactcctga
    19981 taagtttggc aattagacag tagttcttca tgagtacctt gtccaacgat ttttccttca
    20041 tctaaaacaa tgatgttatc tgcttgttgg attgtactga ggcgttgagc aacaatcacg
    20101 agagttttct ccgacatgtg tttatgaagg gctgctctta atgctgcatc tgttttatag
    20161 tctaaagcgg agaaactatc atcgaataca tagatctctg ctggtttgat caacgctcga
    20221 gcaatacata tccgctgttt ctggccccct gaataattag aaccgccttg agcaacaaag
    20281 ctgtccaatc cgtcaggaag ggtgtccact agttcttcta attgagcgat cttgatcgct
    20341 ttagacaact cttctgttga ggcatattca tttcccatct gcaagttgct taaaattgtc
    20401 ccgctgaata agaaagcctt ctgagggaca taactgatgt gggagcggat ctgttcttga
    20461 gtcatttgag taagaggaat accaccaagg gttacttttc ctatcgtagg gtcactaagt
    20521 ctcaagagaa gtttcgccaa actacttttt ccagatccgg tcccgccaac gatcgctgtt
    20581 gtttttcctt taggtattgt gaaatcaata tcttctagaa ctggatcaga gatttcagta
    20641 tatttaaatg tgacatgttc aaaagcagct gcatttgtct gatcaaaggt tggttcagct
    20701 actgttttga tcgtctcttc ctcaatttcg atctctgctt gcagtatttc ttctatccgt
    20761 cgtaatgagg caaacgattt agggataatg accaatacca tagctgcgat catcaagtaa
    20821 gcaagtgtca gcatagagta ttcgactaca gcagtcacga caccgatttg taatgctcct
    20881 tctgctacaa gatttccgcc aaaccacaaa atagaagcat aaacgattcc catgagcaaa
    20941 aaagcaattg gagtgaccca agcaaatatt cggttcactt tgatcatttg attagcatag
    21001 tcagcaaagc tcttattcgt tcgcttttct tcatatgatt gattatcaaa ggcacggatc
    21061 atattaatcc cagtaaagaa ttctctcaat gtgatggtga tccgatccat ttttggctgg
    21121 atactcaaag ataaaggtgc ccctcgtttc atcagtcgat aaacaatcca tacataaaaa
    21181 acaacagaga tcaatgtaac ccatgttagt aatagagaat aacgaaacat catgagaatg
    21241 gtgaaaacgc aaataaccgg tgctggtaag atcatttggc aaaataaaac aatcatttgc
    21301 tggacattat cgacatcatt cgtcattctt gtcaaaagag atccagaact aaaacgactg
    21361 atatccttca ttgacagctg ttggaattta cggaaatatt tctcccgcgt ttggaatcca
    21421 aacctagcgg aagccaatgc tgaaaagtaa ctagaggcaa ctgcagcgat tgttcctaga
    21481 aacgctacaa gcagcatttc aactccaatt ttttttataa ctgaggcttc gccacttgct
    21541 atacctgtgt cgatcagctt tgctactaat tttggtacac ctaatgtacc ggcgatttct
    21601 aaacagatca tcacaaaggt aaggagagcg agtcctttgt ttttctgtaa gaatgttttt
    21661 aagatagaca tattattcct ccttttttcc tgatactttt cgattctata ccttcaagta
    21721 acttgaaggt caatagaaaa aagaataaac tgaaaaagag gtgagaaaaa atgaaaaaaa
    21781 tgtatacgat tggccaagta tcggaactat ttgatttgcc aaaatcaacg atccgctatt
    21841 gggatgaaca aggattaatc cgttcaagta gacaagaaga gaatgattat cgattgtttg
    21901 atatcgatga tatttttatg ctttatgata tcgtttttta tagaaaatta gatataccga
    21961 tcaaacagat gaagaattta tatggaaaaa cattgacgga gttatatgag acacttgatg
    22021 aaacagaaag aagaatccat aaagaactgg tggtgatgaa acaaaaacaa aaagagataa
    22081 gggagcaaaa aaaacagttg aaattgatga tcgatacaaa cgaggaagaa tttcctgtag
    22141 aagagatccc ttttgactgt atgatctcaa cggagtttga agatattgtg gaaatcaaaa
    22201 aatttctacc taactattca agctttggga tgatgagtat gccagcttcc agttcacaga
    22261 cgatgtatgg attttttatc gaccctagtg aagtacattt atttactcaa gacgtgattt
    22321 gggagaaaaa agatacggct gtttatcgtc gattcttatt gaaatccgag atgaaccatg
    22381 cggaacgtaa taatatttta gaaatcagag aaagaatgta tgaaaaagga tggaaaacag
    22441 gagaagtgat cggccagtat ctattgacga atacggatga aaacaatatc agaactgaat
    22501 attatcatgc atggatagaa atgaaaaaat aggaaatatg tgtttaagat aactcgaaaa
    22561 tgatggttgt tttagttata aagaaaaatt ttctggttcg attacgggtt taatgtgggg
    22621 gattattagt cgtggataga tagaatttta gttttttaga ggaaatatgt ctttaaaaat
    22681 ggaatggttg caattcttag cgatatatac gatgtaattg tcctcttaaa aaagtatgga
    22741 aaaataattg tattgggtgt tatgtataat tcatggtgaa ctgagactaa tgagaagaaa
    22801 aattcatatt gttatgagtg agattgtatc agatgagaat gataaattaa ataggtgcat
    22861 aaaagcatta cacttaaaat acattgttgc ttacattatt acaatatgtt aataaggcaa
    22921 aaataaatag atggattgaa taaacatatt ttttaatcaa atagtcccta ctgtgacttt
    22981 cctcagctat cggtgatact accgatagca tttttttgct tataaaataa ttataagtat
    23041 ttagagattt ttcttctaac attcttcaaa caagaaaaat cacaaaggtt attgttattt
    23101 ttatatattc tttttaagaa tgattgtttt tttatagaaa ttttaaaaaa taaaacacgt
    23161 tattgatgga tagttagttt gttatttggt aatattatag aagttgactg gtagtgattt
    23221 atttttcgtc gttttataaa agtgttattt tttggaaaaa ttcacaaata tgtaacgaat
    23281 gtttttctgg aatttgctgt gttaaaaagc agtatgacta atataaatac tattgtcatt
    23341 tatgcaattt tttatggata gtaattttag aggggaatca atgaaggaag aatagaaggg
    23401 aaaaatagtt gcaaatacgc cagttagcaa aaatagcaat acatcttcaa gctttaaaat
    23461 tcgagagaat catcttggta gtggaatcaa ctacaacaaa aggattaaac caattaatag
    23521 atagaattac caatggaaaa atagaaaagg tagttattct atacaaaagt tgtttaattc
    23581 gcttcggtta tgaacttagt gaaacccttt gcaatatatg gtacagaata tagaaattat
    23641 tgacattaca gaaaatgatg aagatttaat acagattgtc actatttgta gatacctttt
    23701 acaaggaaac cgagccaata aagctaagaa gatgattaag gagttgatgg aagacgatac
    23761 aagttaagaa agtgaaacta cgcccaaata aagagcaaaa agagtaattg gacgtggaaa
    23821 atgcaagaaa tgaaccatcg attcggtgga cagtttatta gtaatgacgg tttgagaaca
    23881 tccatgacga gaatgaagga acatcctaaa tgcgtttagt tgaaccaagt gccaaataat
    23941 atggccaaat aagcgatcag ttatcgatga atcccaagta ttctaaacta tatattcatt
    24001 ttgatgctaa gcattgatat atttttttgg gattgatgaa ggttaaccaa tacagggatt
    24061 cactgatgaa attctagagt ctgaccttgg tatcaacgat ttagtgattg tttctaatgg
    24121 aaaagtataa aagtatataa gtacattaac aaaataacta ttagggttac gctccagctt
    24181 gtgcaaggca ctggcaggag gaatcgtgaa atcataatta tggttaagcc acacggacaa
    24241 ctaaaagtca tacaaagcta tcgaatcaat caacagaaga caatcggcaa tcattagctt
    24301 tgtttacagc ttacattgca acgcttgagt tgaaccgaaa atacgaggga ggtaacgcag
    24361 ccattttgag ttggggaatc tttttagaaa attatcgggt accttgttgt ttagtcaaaa
    24421 ttataagggg gagtaatagt gaggaacaaa tttcataaac tgttaagtat gtttctagtt
    24481 atgcttattg tgatcaatca atttgggaac ataacggttc tagctgagtc tttagcagaa
    24541 cagccagagc gttcgctgac gatcgaagtt gatcaagagg aggctgcttt gggtgagcag
    24601 ttccagttgc gagtcagagg aacaccagac ctgctaaatg cattggtcat tgataagagt
    24661 gaggatttga ttgaagggga agtgaagcaa ctcaatgatt acgaacgtct gattacttat
    24721 tcttcccagc attcaggtaa atacgaattg accgcaagaa gcggtgatat tcaatcaaat
    24781 acggtctcaa tagaaatcag tgtaccagaa gaaagcacaa gtgagtcttt taatgtacca
    24841 gaagaaagca caagtgaatc ttttaatata ccagaagaaa gcttaagtga atcttttaat
    24901 gaagccagat catctgagcc atctataacg caggaatctc agattgaaag acaagaggag
    24961 ctcttgaatt atgacatcgg catctctgct gagcgcccca ctttgccgag caggcataca
    25021 caaacgttaa agggatacat tcaaggaggc gctggcgata ttcttgaaga atatggggag
    25081 atggtcgtac gagttcctag agcatcgatt ggcttaattg agttcattag agtggaggtc
    25141 gctgatgatt cacctttaaa gccactggca ttttaccaag aaggcacaga cgttgttttt
    25201 ccatttacct ttgattatga aggattaaat gaagccgctc agtcgtatac attcagtatc
    25261 acgtttgaag ttgctttctt tcaatcaggc ggtactgagc caaataatga ctttcaagtg
    25321 agtcttactt atgctgagcg agcagcaaac acggaatttt caattacgcc tattccttca
    25381 tcaagaccca ttttttcgaa attatactat ggctctagag aaatcgacag ccaaggaaat
    25441 cttgggaatc tgctgattga cgggcaagac gtgaccaaca gtaataaatt tattttgcag
    25501 gtaaattatg aaggatcacg aaaagacggt gtagtggtta ccgatgtact accgcaagga
    25561 acaactttgg caccgaatcc gactacatgg agttccactg gaaatagaga acctatttca
    25621 ggtggggttc ggatcgcaga agtaacgaat ccaggtgaag caaacgttag aactcattat
    25681 gtttccgagg aatttgtgga caaagtatct tttaataagg aaacgaatac acttgttgtg
    25741 gactttggta atctagcaga gggtcaatcg tattttattg aatatgctct tgaagtgacc
    25801 gatgtcgact tgttcaatga aggaagacaa gaggatgggt tttctcggaa tcgagcgagt
    25861 atgacctaca ataatcaggt aatgagccga gactaccgct tgagaatccc tgaaagtaat
    25921 cttccggcaa tctctttaag aaaaagtgtg gacaaaaatg ttatcaatct caatgaaaat
    25981 gaactaacgt atactctgca tgtctcgatg ttttctggaa caagaaatac gcttgttatt
    26041 gaagatgttt tggaagaagg catggaattt gtgtcatttc tatcagatat tccctctttc
    26101 attgatgccg aagtcaataa tggaatagtt cgtttcagtg cgaataatac gattggtggt
    26161 ccgacaagtt ttaccctctc ttttaaggtc aatgtagagg cgtatgcggt cggcgatcaa
    26221 attttaaact ttgcaacgct tgtgaatagt gacaatcgat tgaatacgag tactgtaaga
    26281 acaaggaaaa tcgatgggcg tattaaagta attaaaactg atgagaatgg ccaaaggctg
    26341 gcaggagcaa aatttgaaat tttatcaagc gattcacaag tgatacaaga aggtataacg
    26401 gcagaaaacg gagaattttt aagtgaaccg cttcctgtag gtagctatca agtccgagaa
    26461 acagaagcac cggaagggta tcttttagat gcgacggtac atgatgtttt tataaatgaa
    26521 tactccatag aagaagtgac gttaaccatt gaaaacatcc ttgatacggg tggtgttgaa
    26581 ctaattaaac atgctgaagg cagtggtgaa gtcct
//
LOCUS       DACEEZ010000028        26320 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20313_2017
            SAMN12639966-rid9879223.denovo.028, whole genome shotgun sequence.
ACCESSION   DACEEZ010000028 DACEEZ010000000
VERSION     DACEEZ010000028.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639966
            Sequence Read Archive: SRR10023158
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 26320)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26320)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741683.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741683.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:44:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,016
            CDSs (total)                      :: 2,962
            Genes (coding)                    :: 2,889
            CDSs (with protein)               :: 2,889
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 36 of 73
            Pseudo Genes (incomplete)         :: 29 of 73
            Pseudo Genes (internal stop)      :: 18 of 73
            Pseudo Genes (multiple problems)  :: 8 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26320
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639966-rid9879223.denovo.028"
                     /isolate="E20313_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(<1..1281)
                     /locus_tag="IWO89_001268"
     CDS             complement(<1..1281)
                     /locus_tag="IWO89_001268"
                     /EC_number="2.1.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction-modification system subunit
                     M"
                     /protein_id="HAQ0456035.1"
                     /translation="MGAELNQKLFSAADNLRSKMDASEYKNYLLGLIFYKYLSDRLLE
                     QVVLLADESLEEYDTVSKQTMLYRELLSDEESKEDLIATIVDILGYAIAPEYLFNVLA
                     DQAKQATFQLNDLNKAFVQLASTYNQFNGLFDDVDLQSKKLGTDEQQRNVTITEVIKK
                     LNDVDVLEHDGDVIGDAYEFLISQFASEAGKKAGEFYTPHMVSDMMAQIVTLDQKERR
                     FFSVFDPTMGSGSLMLNVRNYLTHPDNVKYHGQELNTTTYNLAKMNLILHGVDAEEMN
                     LRNGDTLNKDWPTDEPYTFDAVVMNPPYSANWSADTTFLDDSRFNRYGKLAPKSKADF
                     AFLLHGFYHLKETGTMAIVLPHGVLFRGAAEGVIRQKLLEDGSIYAVIGMPANLFFGT
                     SIPTTVIVLKKNRQTRDVLFIDASREFVKGKNQNK"
     gene            complement(1791..2573)
                     /locus_tag="IWO89_001269"
     CDS             complement(1791..2573)
                     /locus_tag="IWO89_001269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0456036.1"
                     /translation="MENSSNSAEVNVSTSTSEVVKCSQETTSLPEELLGDWQINNKDA
                     GIEISDSKAVFRIGRSQVESKDYYVDVNNKMLLHVYFSFEYDSVKYIEVSIERTGYSE
                     FISKSVGILTMHTKDGQYISLGVKRVSGENNETEAVTEQTMDERIDSSKSNETEVDTG
                     KTIDERIDSFIETYTAIPPTWKLVNEDKGITINTDTEISTDAQSLSDLLANLTGISNA
                     VTEQVGKDIPLRLLENGKKDYSVVFKNGQVTDYNEVFKKVIE"
     gene            complement(2638..3474)
                     /locus_tag="IWO89_001270"
     CDS             complement(2638..3474)
                     /locus_tag="IWO89_001270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAQ0456037.1"
                     /translation="MRVIKTLNNSLILCLDKQGKEVILMGKGIGFQLKKGDLVPADKI
                     QKVFKLDSLSDALKKDYIKLFEAAPDDLIICVKRIIDYANAKFDFKLNKSLFFTLVDH
                     LKHAIERSQKGIVFQNRLLIEIQKYYPKEFEIGCYAIQLINKKMNVQLPEEEAGNIAF
                     HIVNAQSEQANMEETMLSVRALKDILAMLGYLFPEKKVDKDSMLYLRFVTHLQFFIAR
                     MIKKEELPEKDDFLLASVKQRFPKEYRAALKIKEYVQEEMGASANQDELLYLTLHISR
                     VY"
     gene            complement(3492..4901)
                     /locus_tag="IWO89_001271"
     CDS             complement(3492..4901)
                     /locus_tag="IWO89_001271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010757186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HAQ0456038.1"
                     /translation="MQYKQLSAFPEKFLWGASISAYQAEGAYQEDGKQPSIIDKYQHT
                     EGVANFEVASDFYHHYKEDIQLFKELGLKAFRFSIAWTRIMDIENNKTNQKGISFYHQ
                     VIDECLNQGIEPIVTMYHFDLPYFLEEQGGWLNRATIDYFVDYVQVLLTEYGDKVNYW
                     LTINEQNTMILHPGAIGLPPSGELPSKKELFQINHHVLLAQARVIHLYHQLNLKGKIG
                     PAINLTAMYQATSSPEDAIAAHNWETLRGWSFLDVAVRGKYNYLFENYLNDRGLYPKI
                     EAEDQAILSSGKPDFIAINYYSTATIAASKNDGSDVSARAGDQQIMLGEEGVYRAAEN
                     PYVDKTPYGWVVDPTGLRLTLRKLYDRYDLPILITENGYGAPDIVEEDRRINDQDRID
                     YLAKHILAIQAALTDGVDVFGYLPWSAIDVVSTHQGFNKRYGFIYVDRTDENLKELKR
                     IKKESFFWYQKVIARNGLA"
     gene            complement(4915..6738)
                     /locus_tag="IWO89_001272"
     CDS             complement(4915..6738)
                     /locus_tag="IWO89_001272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAQ0456039.1"
                     /translation="MDYREVGRNVLQQVGGKENVVSLTHCATRLRFELKDKRKADTKA
                     LEKTLGVISVVDSGGQYQVVIGNEVQTAFKEIQKEVGTSIKKGPSSDNQESIISRLIS
                     VISTTFTPMIPAITGAGMVKAILAVLTLTGVLSDSSQTYIILNTIADAAFYFMPVLLA
                     YGAAIKFDVNPILAITVAGILLHPNISQLFSTGDAVSFLGIPVLAADYAGSVLPIIFT
                     VWIMSYVEPFADRISPSFIKFFTKPMLVFLIMGPLALVVIGPFGTLLNDLIAMGAEWI
                     NGKAAWLIPFLMGGLQPFLVVTGTAWAMTPIATMQLSSTGSEMINGPGMLASNIAQSG
                     ATFAVAAKTKNKELKQLAVSTGITALMGITEPSLYGVTLKLKKPLVASMIGGAIAGLY
                     AGLSGLVRYAFVSPGLAALPAFIGENPMNIVHAIITCVIAFVATFIIAYLIGFDDTSD
                     ESEQTAIAEAVIVENPVKGTVCPISEVNDAVFSKGLLGKGVAIHPEENSIYSPVEGEV
                     ITILESKHAIGLRSKEGIELLIHVGIDTVNLKGEPFEVFVKAGDQVSAGQLLINADFA
                     AIRNAGYDDTVIVVVTNSENYAGISEASGSLKEHEPLMQIS"
     gene            complement(7581..7907)
                     /locus_tag="IWO89_001273"
     CDS             complement(7581..7907)
                     /locus_tag="IWO89_001273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0456040.1"
                     /translation="MNKVNMNKKFVIIGIVVLAIIGIIFSKMTGTSGLEKSSKPLVTQ
                     ILQEQYNLNRSCDDVTITHENGDNTYRAKAILDNGSAININIEYYPKKDRIYVEIPYA
                     EVLMLN"
     gene            complement(7953..8315)
                     /locus_tag="IWO89_001274"
     CDS             complement(7953..8315)
                     /locus_tag="IWO89_001274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010717431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HAQ0456041.1"
                     /translation="MYCRKCGKEITDESKYCQFCGALQETDETTGTDTVKKSTEQTIN
                     EMKEQIVHGQETGKLYLIIGWISMVVSLAFIPVVFGAVAVIMGYLYREKDEKMGTILM
                     IAGVAGAIFGVLLGMSMY"
     gene            complement(8352..8567)
                     /locus_tag="IWO89_001275"
     CDS             complement(8352..8567)
                     /locus_tag="IWO89_001275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAQ0456042.1"
                     /translation="MKKVSNLSRYRKEKGLSQTELAKKMNVTQQCVSSWQTGRTIPKP
                     YQMKMLSEILSVPMNELFSDVFNKVNS"
     gene            8694..9029
                     /locus_tag="IWO89_001276"
     CDS             8694..9029
                     /locus_tag="IWO89_001276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAQ0456043.1"
                     /translation="MKQKQYAKVIREKRKALGWTQKELAEKIFSTQQAVARWENSVTE
                     PNLDSLTALSRALGTPVSHFLDNVVVDYEEEFLALYRSLSTEDAVRTIDYMKLLKRQE
                     NERNQLLKD"
     gene            complement(9105..11102)
                     /locus_tag="IWO89_001277"
     CDS             complement(9105..11102)
                     /locus_tag="IWO89_001277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0456044.1"
                     /translation="METSMINIPIYVDQEKLLEKPVTKKEKTDVRDRCLDNYREVTRD
                     EFVEILNSSQSFIPSKTKNKGNDKESFVETRVIILDVDNTVKDEKGKVVDLSKDDSRY
                     LSVEKVLSINLVHNSAFAIQKSIRYSENLEKYKIVFLLKEAITDYGKMVAVYEYLKKN
                     IPGCDDNVNASNRMFFGGYKSDAVIIINEDNMLDITELPIDFTMTCSKNCQSDSGREY
                     ELVNETDFVKLIKNDDKEEMKQWFKDCLFDSSDMSFNEIYERLLTIDMNKLLKSSKNL
                     RCLFHNDHNPSASIFTSKTGHSIFYCHSSNCDISENFIGVVMLILKKDSRVETFNWLL
                     ENLDLYPTHFRKLKEESKVLFDTLESMKDKLFRTIRNYLEEMRRVYDVLLSDVNFFDD
                     SKESVTCILSGEQLAKKMSSYYGKTYDIDKCNKLLSLMTFIGLIKKLDDEDVPLKMLE
                     YLNRLKEEKALGNFNHKRSNVYRLDVFNATSVIQKLNEDILPLLEKIHFTYQYFSYEW
                     VKICFNEKEAKRVFPQNSRSELSKEKQLILDEAHEVLQGLSWQGTYLMTEKELINTVC
                     ERLRNVGDNSVKNALIKNRGYFVKQGFTLERASKDIKKLYNVKASSSFLIYLAKGAGI
                     DETIKELILSGAGIVPLYQFTSVTRDDGRRVRKRKDPIVLN"
     gene            complement(11200..11475)
                     /locus_tag="IWO89_001278"
     CDS             complement(11200..11475)
                     /locus_tag="IWO89_001278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAQ0456045.1"
                     /translation="MSLQIIDSIVSIQVEQQVKEIITVLKPMLEDIISGVTLEYMDYQ
                     QASEYLGVSVGTIRKYVSQYGLPVIKIDTVIRFKKSDIDSFMERYKG"
     gene            11707..12441
                     /locus_tag="IWO89_001279"
     CDS             11707..12441
                     /locus_tag="IWO89_001279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAQ0456046.1"
                     /translation="MIKRPPINYLERKKILGTKIKAIRKSKKLTQPAFGLMINNGQLI
                     DKKTIYEWEKGTYLPIPERLSRIADLGNMSIEELVCGNVEEYILGIILYRDSIVLDGI
                     TFPDKNLFQHLRQQFPPVHSNLDTWLDRYSKLEPEMQEFIANKTCNKVKNEKISLFNI
                     LKIEELFINAIVEEFDNNILFLTSSIEELLERMVDEWLPIQLKDMSYPEEAVREITDN
                     INKLEQTISSIGKKYTKKKMKGGDTI"
     gene            12480..13622
                     /locus_tag="IWO89_001280"
     CDS             12480..13622
                     /locus_tag="IWO89_001280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HAQ0456047.1"
                     /translation="MAKISKYKKKNGQVAWMYKGHIATDPRTGEKINTTRRGFSSKQE
                     AQKDYDEYRHQILYGVKKKAPDMTFEDLYNEWITHQRTSVKASTIAISVRYANNQILP
                     AFGKLKLSNISVPYCQKVVDEWHSKYESYDYMRKQTAQILRYGVAMQYIDNNPMEKTL
                     LPRKKEYEKNRKFYSKEELNNLLDAFKDFGNMKQYAFFRLLAYTGMRKSEVLALQWKD
                     IDTFNKELHVNKTLAVDEFGKVIIQSPKTRASRRVISLDTETLSILNNWKLQQKEEYL
                     KLGYNTSSKEQHVFTTVKNTLYIPNTVNDWLRYILKKYNLPRITPHGFRHTHASLLLE
                     AGESVKVVQQRLGHENSKVTLDIYAHITNNAPKKTGQDFADMMAHQ"
     gene            complement(13717..15108)
                     /gene="sufB"
                     /locus_tag="IWO89_001281"
     CDS             complement(13717..15108)
                     /gene="sufB"
                     /locus_tag="IWO89_001281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufB"
                     /protein_id="HAQ0456048.1"
                     /translation="MGVPELEEYKFGFHDDVEPVFSTGEGLTEEVIREMSRIKGEPEW
                     MLEFRLKSLETFNKMPMQTWGPDLSDIDFDAIKYYQKPSDKPARDWEDVPEKIKDTFE
                     KIGIPEAERAYLAGASAQYESEVVYHNMKDEFEKLGIVFTDTDSALKEYPELFKEYFS
                     KLVPPTDNKLAALNSAVWSGGTFIYVPKGVKVDVPLQTYFRINAENTGQFERTLIIVD
                     EGASIHYVEGCTAPTYSSNSLHAAIVEIFTRKDAYCRYTTIQNWSDNVYNLVTKRAKA
                     YEGATVEWIDGNLGAKTTMKYPSVYLDGKGARGTMLSIAFAGANQIQDTGAKMIHNAP
                     NTSSSIVSKSISKDGGEVNYRGQVTFAKNSAGSISHIECDTIIMDEWSKSDTIPFNEI
                     HNSQVSLEHEAKVSKISEEQLYYLMSRGLTESEATEMIVMGFVEPFTKELPMEYAVEL
                     NRLISYEMEGSVG"
     gene            complement(15113..15583)
                     /locus_tag="IWO89_001282"
     CDS             complement(15113..15583)
                     /locus_tag="IWO89_001282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SUF system NifU family Fe-S cluster assembly
                     protein"
                     /protein_id="HAQ0456049.1"
                     /translation="MGLSKLDNLYRQVILDHSSHPHHRGSLTDSTSKIELNNPTCGDV
                     IELQVDVKDGIIKNIAFDGSGCSISTASASMMTDAVIGKSIEEAEHLTVDFLQLVQGK
                     EVKDPDQLGDAAMLGGVAKFPARIKCATLAWKALGKAMENDGTAAFHHYHKEEE"
     gene            complement(15570..16805)
                     /locus_tag="IWO89_001283"
     CDS             complement(15570..16805)
                     /locus_tag="IWO89_001283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HAQ0456050.1"
                     /translation="MIDAKKLRNDFPILFQDVNDEPLVYLDNAATTQKPKQVLQALAY
                     YYEHDNANVHRGVHTLAERATHDYEQAREKVRAFIHAKETAEVLFTRGTTTSLNWVAR
                     TFGDRYIKAGDEIVLSYMEHHSNVIPWQQLAKRTGAVLKYIELTEEGFLDMEDAAKKI
                     TDKTKIVSIAHVSNVLGVFNPVQELAELVHENGGVIVVDGAQSAPHMAIDVQALDCDF
                     FAFSGHKMCGPTGIGVLYGKRKWLEEMEPVEFGGEMIDFVELYDSTWKELPWKFEAGT
                     PNIAGAIALGHAIDYLQKLGMDNIHRYEEELAAYVLPKLQAIDGLTVYGPQDPNFRTG
                     VVAFNLDGLHPHDVATALDMEGVAVRAGHHCAQPLLKYLEVSATARASFYFYNTTQDA
                     DRLVEAIMATKEFFQHGTI"
     gene            complement(16802..18088)
                     /gene="sufD"
                     /locus_tag="IWO89_001284"
     CDS             complement(16802..18088)
                     /gene="sufD"
                     /locus_tag="IWO89_001284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufD"
                     /protein_id="HAQ0456051.1"
                     /translation="MKEKNWLDYLDAVNDFSLSKGEPDWMRTFRQDALAKADELPLPH
                     IDRVKFHRWSLFDVKETQTISETGTIPAFDAMKDNPVLVQQGSWTIFEQLPVELAEKG
                     VIFTDLFTAMIEYPELVQEYYMKKAVNMNEDQLTALHVAFMNSGIFLYVPKNVVIDEP
                     LESLFIQDGASDEHFFKHVLIVADEHSEFSYLERFQTTKEQVAKSSGNIIVEVIAKAG
                     SKIKYSAVDQLGENITSYMNRRGHILRDASVDWAIGVMNDGHVIADFDSDLAGEGAHA
                     EVKIVAISSGRQIQGIDTRVTNKAPHTIGHILQHGVIREKGTLTFNGIGHILKGAKGA
                     DAQQESRVLMLSDKARGDANPILLIDENEVTAGHAASVGRVDPEEMYYLMSRGLHKEE
                     AERLVIRGFLGSVLTAIPVEQVRKELVEVIEGKLNG"
     gene            complement(18101..18874)
                     /gene="sufC"
                     /locus_tag="IWO89_001285"
     CDS             complement(18101..18874)
                     /gene="sufC"
                     /locus_tag="IWO89_001285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly ATPase SufC"
                     /protein_id="HAQ0456052.1"
                     /translation="MSVLEIKNLHVSIEEKEILKGVNLTMKTGEIHAIMGPNGTGKST
                     LSAAIMGNPNYEVTEGEILFDGKNVLDLEVDERARLGLFLAMQYPSEIPGITNAEFMR
                     AAINAKRDENDKISVMAFLKKLDEKMALLNMPEEMAERYLNEGFSGGEKKRNEILQLL
                     MLEPTFAILDEIDSGLDIDALKVVAKGVNEMRGDNFGALIITHYQRLLNYITPDVVHI
                     MMEGRVVMTGNADLAKRLEAEGYAGISKELGIDYKEEEA"
     gene            complement(19071..19919)
                     /locus_tag="IWO89_001286"
     CDS             complement(19071..19919)
                     /locus_tag="IWO89_001286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HAQ0456053.1"
                     /translation="MKKKIFGFAAALLLTVGLAACGNNSDSKDSANKADDTTLKVGAS
                     PTPHAEILEHVKPLLKDEGIDLEIVKFDDYVLPNQSLEEGDIDANYFQHIPYLNKEIK
                     EKGYDFVNAGAVHIEPMGLYSKKVKDVSELKDGATIITSNSESDWGRIITILQDAGLV
                     KVKDGVDLETATFDDIEENPKNLKFNHTIDPSLLASTYQNDEGDLVAINANFAYGAGL
                     NPLEDAVLLEKDNSPYVNIIATRKGDEDSDKIKKLIEVLHRKEVQEWITDKWGGSVKP
                     VKADAK"
     gene            complement(19943..20629)
                     /locus_tag="IWO89_001287"
     CDS             complement(19943..20629)
                     /locus_tag="IWO89_001287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAQ0456054.1"
                     /translation="MDKSFSETYLDFKQINPETMNQAILDTLYMTLISMVLVTIIGLV
                     LGLILYSIGRKNKPYARIIYGIVSIISNIFRSTPFMILMVLIIPFTKAIVGTMLGAQA
                     AIPALVLSAAPFYARLVEIAFREVSPGVLEAADAMGASYWEIIWKVLIPESIPALISG
                     LTVTTISMIGFTAMAGAIGAGGLGGLAWQEGYQRGNLTVTFVATLIILLIVFIVQGIG
                     DFLTKKTDKR"
     gene            complement(20622..21656)
                     /locus_tag="IWO89_001288"
     CDS             complement(20622..21656)
                     /locus_tag="IWO89_001288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAQ0456055.1"
                     /translation="MSLIQLTNVKKTFNGKNGNIQAVNDVSLNVEKGDIYGIVGYSGA
                     GKSTLVRLLNGLELPTSGEVIVNDQDITKLKNKELRTFRKKIGMIFQHFNLLWSRTVL
                     ENIQLPLELAGVPKSKRKERAEELLRLVGLEGRGQAYPSQLSGGQKQRVGIARALAND
                     PEILLCDEATSALDPQTTEEVLDLLLAINKKLNLTIVLITHEMNVIRKICNKVAVMEL
                     GKVVEEGDVLTVFRQPKKEVTKRFVQQDIEPEADSTELLAELIKENPSGRLATLTFSE
                     RNANEPVISQAIRRYNIDVNVVYGKIKQVKEGSFGSLTVLMTGETKELDQAEVFIKEQ
                     GVGIEVIHRG"
     gene            complement(22051..22389)
                     /locus_tag="IWO89_001289"
     CDS             complement(22051..22389)
                     /locus_tag="IWO89_001289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein H"
                     /protein_id="HAQ0456056.1"
                     /translation="MAGKHLKKKDNLWILFNGKEYCVGLTNEAQEELGDITFANLPKA
                     GQSLKKDEPLIEVEAEKAVNEFASPLTGTVSSVNDKIGEDINILNDSDEMNAWILSFK
                     DVDPKEFDEL"
     gene            complement(22391..22747)
                     /locus_tag="IWO89_001290"
     CDS             complement(22391..22747)
                     /locus_tag="IWO89_001290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase family protein"
                     /protein_id="HAQ0456057.1"
                     /translation="MYTFYWYPKCSTCKKAKAWLDQHGVSYQTVDMIKEVPKASTIET
                     WLETSQVPIRRLFNTSGMKYRELGLKDQVGEFTASKASQVLSTDGMLIKRPLILREDQ
                     LVAIGFNEKIYEGELI"
     gene            complement(22753..23940)
                     /locus_tag="IWO89_001291"
     CDS             complement(22753..23940)
                     /locus_tag="IWO89_001291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HAQ0456058.1"
                     /translation="MEKKLKASNDNRIDYGVILPVFLLCLIGLLSLYVALSHDPNNPA
                     VLKGVGMQVVWYLVGAAAIVVIMHLNSKWIWKLTPYLYGLGLVVMGLLLKFYDQNLAA
                     STGSKNWFRFGAFTFQPAELMKIAYILMMALIVTKHNTQVKERTMKSDFWLIGKLLIV
                     TIPVLALIMAQDDFGTMLVFLAIFGGIFLMSGISWRIIAPVVILAVVVGAGTIFLVTT
                     EGGRDLLYKVGFKSYQFARIDSWLDPFHDTSGMSYQPAQGLLAIGTGGLFGKGFNVSN
                     IYVPVRESDMIFTVIGENFGFIGGAFVIFLYFILIYRMIRVCFDTNNEFYAYIASGLI
                     MMLLFHVFENIGANIGLLPLTGIPLPFISQGGSSILGNMIGIGLILSMRYQNEAPARS
                     SRR"
     gene            complement(24131..24976)
                     /locus_tag="IWO89_001292"
     CDS             complement(24131..24976)
                     /locus_tag="IWO89_001292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HAQ0456059.1"
                     /translation="MFKLLTTFRAVYETRNFSRAAEVLFLSQPAVSNQIKQLERELNI
                     DLFQRNGRKEMLPTKQADLLYERTLVLLEEWEETQQKLLNEQNEEEVCRIVASHTFAV
                     YLLPQLMKHLIKRFPKVHFEIEITNSHESLEQVAKHESDFGFIEKPLETSGVQRYSLM
                     EDQLVIAGDPKSKLWLVREANSGVFHYTQRYFEEMNIQDKKMIVKSNDVIVALLKEGI
                     GRSILSKRSVPENVPMTPLNFHRYFYFIQRTNLVSDQLLEIAEEILRYNESTETSHSE
                     ENLSW"
     gene            25210..26175
                     /locus_tag="IWO89_001293"
     CDS             25210..26175
                     /locus_tag="IWO89_001293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TDT family transporter"
                     /protein_id="HAQ0456060.1"
                     /translation="MKNFLKIIPIPICGLILGLASLGNLFKDYHHVALGNLTGGISMI
                     LMIFIFVKLFILFEHTKQTLHDPIIASVSPTFTMSLMVICTYFVSYESIAPIVKFIWL
                     CAVIFQVILVFYFNYHHVIKADLSIEAIYPSWFIIYVGFGVITVTAGNFFPLIGKIFF
                     WISLICYAFLLPIIIKRVFFVRNMAHPTRPLITIVAAPGSLCLTGYLKNFAEPNFYLV
                     VALFALSQVLYFVVLSMLPRLFKLRFYPSYAAFTFPLVISATAIFTTVHYFNSLGIYS
                     SVLDFLKVAECLFASVVVFYVLAHYVRFLIKEHSKHGKFVSKKIA"
BASE COUNT         7694 a         5536 c         4463 g         8627 t
ORIGIN      
        1 tttattttga tttttccctt taacaaattc acgactagca tcaatgaata atacatctcg
       61 agtttgacga ttcttcttca acacaatcac cgtcgttgga atcgatgtcc caaagaacaa
      121 gttcgcaggc ataccgatca cagcataaat actgccatct tctagtaatt tttgacggat
      181 gaccccttct gcagcgccac ggaataaaac tccgtgtggt aagacgatgg ccatggtacc
      241 tgtttccttc aaatgataga acccatgaag aagaaacgca aaatctgctt tggattttgg
      301 cgctaatttc ccataacgat taaaccgaga atcatctaag aaagtcgtat ctgctgacca
      361 attagccgaa taaggtgggt tcattactac tgcatcaaat gtgtaaggtt catctgttgg
      421 ccagtcctta ttcaacgtat ccccattgcg gagattcatt tcttcggcgt cgacaccatg
      481 taaaatcaag ttcatttttg ctagattgta tgtcgttgta tttagttctt gcccatgata
      541 tttaacgtta tctgggtgtg ttaagtagtt acgtacgttt aacatcaatg aacctgaacc
      601 catcgttgga tcaaaaacac taaagaatcg tcgttccttt tgatcgagcg ttacgatttg
      661 cgccatcata tcagaaacca tatgaggtgt gtaaaattct ccggctttct tccctgcttc
      721 tgaagcaaat tgactgatca gaaactcata cgcatccccg atcacgtccc catcgtgttc
      781 taaaacatct acatcattca atttcttgat gacttcggta atcgtcacat ttcgttgttg
      841 ctcatctgtt cctagttttt ttgattggag atccacatca tcaaataacc cattaaattg
      901 attgtaggta gatgccaatt gcacaaaggc tttgtttaga tcgttcaatt ggaacgttgc
      961 ttgttttgct tgatccgcta agacgttgaa aagatactct ggtgcaattg catatcccaa
     1021 aatatcaacg attgtggcaa tcagatcttc ttttgattct tcatctgaaa gtaattcacg
     1081 atacaacatt gtttgtttgc ttactgtgtc atattcctct aaggattcat ctgctaaaag
     1141 aaccacttgt tccaacaatc gatcggataa atatttatag aaaatcagtc ccaataaata
     1201 atttttatat tctgacgcat ccattttact acggagatta tctgccgcac taaataattt
     1261 ttgatttaat tctgcaccca aaaagataca ctcctatctc ttatttaaag ttgctatata
     1321 ccctcttatt ttagcatacc cccttttata aaaagcaaaa aaaaaaagaa agaaacttat
     1381 tcttgctaat aaactacgcg aaaggagtct aacgttgttt gatataactt agactgatcg
     1441 ttaacttatt ttttatattt ctacatagtt atctttagaa ccatttcaga tacatatgaa
     1501 acaattacat atgatttttt cataaaaaaa cagtactgtc tagtagtata aaatatacaa
     1561 atttcttaaa aattttttca ctaaaatcac tacctgaaac agtctaaaac acttatatat
     1621 caacgtttaa tgaaactatc tccgtttcat ttcagataca aataaatata gtcgggctcc
     1681 aaatccgaaa aaaaataccc ccagtagctt atcctaagct actggagatg tataagtacg
     1741 gtcatataac aacggggtag ggtaaataaa tgaaaaagat ggaagaatca ttattcaata
     1801 acttttttaa atacttcgtt gtaatcagtt acctgtccgt tcttaaatac cacagaataa
     1861 tcttttttcc cattttcaag taaccgaagt ggaatgtctt taccaacttg ctctgtcact
     1921 gcattactga tacctgttag attagctaat aaatctgata aagattgagc atctgtagaa
     1981 atctctgtat cagtatttat cgtgattcct ttatcttcat ttacaagctt ccaagtaggt
     2041 ggtatagctg tgtatgtttc gatgaatgaa tcaattcttt catctatcgt cttcccagta
     2101 tctacttctg tctcattact cttagatgaa tcgattcttt catccatcgt ctgctccgta
     2161 actgcttctg tctcattatt ctcaccggaa actctcttca cacccaaact tatatattgt
     2221 ccatctttag tgtgcatagt taaaattccc acgcttttac taataaattc actataacca
     2281 gttctttcaa tagagacttc aatgtatttc acactatcat actcaaaaga aaaatagaca
     2341 tgtaaaagca ttttgttgtt tacatctaca taataatcct ttgactctac ttgagacctt
     2401 cctatacgga atacagcttt actgtctgat atctctatac cagcatcctt attattaatc
     2461 tgccaatctc caagtagctc ttctggcaag ctagtagttt cttgtgaaca ctttaccact
     2521 tcactggtag aggtcgaaac attcacctcc gcactgttag aagaattttc caaggaatca
     2581 ggagccttag cacaaccaac aagtctttat atattgtgca gccgatattt tcttcattca
     2641 gtacaccctt gaaatatgca gtgttagata aagcagttcg tcctgatttg ctgaggcacc
     2701 catctcttct tgaacgtact ccttgatttt caaggccgcc cgatactctt tcggaaaacg
     2761 ctgcttcact gaagccagca aaaaatcatc cttctccggc aactcttcct tcttaatcat
     2821 tcgggcaata aaaaactgta aatgcgtcac aaatcttaag taaagcatcg aatctttatc
     2881 aacctttttt tcgggaaaca gatatcctag catagccaag atatccttta gtgctcttac
     2941 cgagagcatg gtttcttcca tatttgcttg ttcactttgg gcatttacaa tgtgaaacgc
     3001 aatgtttcca gcctcctctt ctggcaactg aacattcatt tttttgttga tcaattggat
     3061 agcatagcag ccgatctcaa attcttttgg gtaatacttt tgaatttcaa tcagcaggcg
     3121 attttgaaaa acaatgcctt tttgactgcg ttcaatggca tgcttcaagt gatcaaccaa
     3181 ggtaaaaaat agacttttat tcagcttaaa gtcaaacttg gcgttggcat aatcgataat
     3241 tcgcttgacg caaataatta gatcatccgg agcagcctca aacagtttaa tgtaatcctt
     3301 ttttagagca tcagaaagtg aatctaattt aaatactttt tggattttgt cagccggaac
     3361 caaatcgcct ttctttagtt gaaatccaat ccctttcccc atcaaaatta cttctttgcc
     3421 ttgcttatct aagcaaagaa ttaaactgtt gtttaacgtt ttaattactc tcattagatt
     3481 caccgttctc tctaagctaa gccatttctt gcgattactt tttgatacca aaagaagctt
     3541 tcttttttta ttcgttttaa ttctttaaga ttctcatcgg ttcggtcaac atatataaaa
     3601 ccataacgct tattgaagcc ttgatgggta ctaacaacat caatcgctga ccaaggtaaa
     3661 taaccaaaaa catctacacc atctgttaaa gctgcttgaa tagccagaat atgtttggct
     3721 agataatcaa ttcgatcctg gtcattaatt cgtctatctt cctcaactat gtcgggtgcc
     3781 ccatagccat tttcagtaat taaaataggt aaatcatagc gatcgtacaa tttcctcaat
     3841 gttaatcgta gtccagtagg gtcaactacc cagccatatg gcgttttatc tacataagga
     3901 ttttcagctg cccggtagac accttcttct cccaacatga tctgttgatc ccccgctcta
     3961 gcggaaacat ctgatccgtc atttttgctg gcagcgatgg tggcagttga ataataatta
     4021 atcgcaataa aatcgggttt accggaggat aaaattgcct gatcctctgc ctcaatttta
     4081 ggatataatc cacgatcatt caaatagttt tcaaacaaat aattgtattt tccccgaact
     4141 gctacatcta agaaagacca gcctctcaat gtttcccaat tatgggctgc aattgcatct
     4201 tctggactgg aggtagcttg atacatagcc gttaaattga ttgctggacc gatttttcct
     4261 ttaagattca actgatgata caagtgaatt actctagctt gagctaataa aacatggtga
     4321 ttaatttgaa agagttcttt ttttgaaggc agctctccac ttggtggcaa accaatcgct
     4381 ccaggatgaa ggatcatcgt gttttgctca ttaattgtca gccaatagtt caccttgtca
     4441 ccatactcag taagcaatac ctgtacatag tccacaaaat aatcgatagt tgctcgattt
     4501 aaccaaccgc cctgctcctc tagaaaataa ggtaaatcga aatggtacat ggtgacaatc
     4561 ggttcgattc cctgattcag gcattcatca attacttgat gataaaaaga aattcccttt
     4621 tgattggttt tattattttc aatatccata attcgcgtcc aagcgatgga aaagcgaaaa
     4681 gcttttaagc ctaattcttt aaacagctgt atatcttctt tatagtgatg gtaaaaatcc
     4741 gaggcaactt caaaattagc taccccttct gtatgctgat acttgtcaat aatagacggt
     4801 tgtttcccat cctcctgata ggcaccctct gcctggtagg cactaatgga ggcaccccat
     4861 aaaaacttct ctggaaatgc tgaaagctgt ttgtattgca caaaaatcct ctccttaact
     4921 aatttgcatc aatggttcgt gttcctttaa gcttccgctt gcttcactaa ttcctgcgta
     4981 attttcactg ttggtcacta ccacaatcac tgtatcatcg tagccagcgt ttctaatggc
     5041 agcgaaatcg gcgttgatta ataattgtcc tgctgacacc tgatctcctg cttttacaaa
     5101 tacttcaaag ggttcccctt ttaaattcac ggtgtcgatc cccacatgga tcaataattc
     5161 aataccttct ttagaacgta gaccaatcgc atgtttagat tctaaaatag taatgacttc
     5221 tccctcaaca ggtgaataaa tgctgttttc ttctggatga atggctacgc cctttcccaa
     5281 tagtccctta gaaaagacag catcgttcac ttcagaaata ggacacacgg ttccttttac
     5341 cggattttca acaatgactg cttcggctat tgccgtttgc tctgattcat cagaagtatc
     5401 gtcaaatcca attagataag caatgataaa ggttgcaaca aaggcgatta cacacgtaat
     5461 aattgcgtga acaatattca ttggattttc tccaataaag gctggtaagg ctgctaagcc
     5521 aggggaaaca aaggcatatc tgactaaacc gctcaagcca gcatacaaac cagcaattgc
     5581 accaccaatc atggaagcaa ctaatggttt tttcagcttc aaggttactc catagagtga
     5641 aggctcagta attcccatta aagcagtgat cccagtggac acagctagct gcttcaactc
     5701 tttgttttta gttttcgcgg ctacagcaaa ggtagcacct gattgagcaa tattagaagc
     5761 cagcattcct ggcccattga tcatttcaga acccgtactg ctcaattgca tagtagcgat
     5821 tggcgtcatt gcccaagctg tacccgtaac caccaaaaat ggctgcaaac cacccatcaa
     5881 aaatggaatt aaccaagctg ctttgccgtt aatccattca gcccccattg ctataagatc
     5941 gttcagcaac gttccaaacg gtccaatgac aaccaacgct aaaggcccca taattaaaaa
     6001 taccagcatc ggcttagtaa agaatttaat aaacgaaggc gaaattcgat ccgcaaaggg
     6061 ctctacatag gacatgatcc aaacagtaaa gataatcggt aaaactgatc ccgcataatc
     6121 tgcagctaat accggaattc ctagaaaaga aacggcatcg ccagtagaaa aaagctgtga
     6181 aatatttgga tgaagcaaaa tgccagcaac agtaatcgcc aaaattgggt tgacatcaaa
     6241 ctttatggct gccccatagg ctaatagtac tggcataaaa taaaaggctg catcagcaat
     6301 agtattcaaa atgatgtaag tttgactact gtctgatagt acaccagtca aagtcaagac
     6361 ggccaaaatg gcttttacca tcccggcacc tgttatagct ggaatcatcg gtgtaaaggt
     6421 ggtagaaatg acactaatca atcttgaaat aatactttct tgattgtctg aagaggggcc
     6481 ctttttaatt gaagttccca cttccttttg aatttccttg aaggctgttt ggacttcatt
     6541 gccaatcacc acttggtatt gtccaccgct atccaccaca ctaattacac caagtgtttt
     6601 ttctaaggct ttcgtatcgg ctttcctttt atctttgagt tcaaaacgca gtctggtggc
     6661 acaatgggtt aagctgacca cattttcttt tcccccaacc tgttgcaata catttcttcc
     6721 tacttctctg taatccatta ttctctctcc ttaaagcagt aaaaaagacc aagtcaaacc
     6781 acgccccaat caacattgaa gtgttagttt gtcttggtct tgcctgcata atcagtaaca
     6841 tgccaataac gttattttgc tgtaatccaa taatagcaca ttatgatagc gcaatcaaca
     6901 gggaatttaa ttttttatct tttcgtttga ccgtataggg taatataaca gttagatcac
     6961 atcaactagg gtatatctgc gtatacttac tgctaaaata agtatatcta atattaacta
     7021 taccagtaaa accattccta cataaccagc gggaaattcc caaaatcgtg aaagcatcaa
     7081 aaatgcatat aacggatcac acaacttcaa caattctgct cttgggaaaa attgatgaat
     7141 ttggtgagta aaggatggaa taaacagcat tccaacaata taggcaacaa tagtgatact
     7201 tgtttttttg gtaaaaatca gtaagcaata gccaatttca atcattccga gatataaagg
     7261 tatcgataga atgatttggg ctagtattct taaaaattaa ctagcaaatg tttaaactga
     7321 tttatttcta atctacatgt agtaagtatt tcaattccac caattatcaa taaaaatacc
     7381 aaacatatcc ataataattt tttctttttc atttctttta tattgttata acagtgaata
     7441 acataaaaat tcccgacaat cattgtaata tttttttgtc acctgtatta gtatttaatt
     7501 tataggaaac tataaaaact ccctagtgaa tttacacctt tgggagttcc tgtttggacg
     7561 gtatgcataa tacatttaaa tcaatttaac attaacacct cagcatatgg tatctccaca
     7621 tatatacggt ctttctttgg atagtactca atatttatgt tgatagcact tccattgtct
     7681 aatatagctt tagcccggta tgtgttatct ccattttcat gagtaatcgt cacatcatca
     7741 catgatcgat ttaagttgta ctgttcttgt aaaatctgtg ttaccaatgg ttttgatgac
     7801 ttctctaatc cagatgtacc tgtcattttt gaaaaaataa tacctataat agccaagaca
     7861 acaataccta taatgacaaa cttcttgttc atatttactt tattcaactt tttatctcct
     7921 atctccaaaa taatttgctc ttatccctct tatcagtaca ttgacatccc taaaagtaca
     7981 ccaaagattg ctccagctac ccctgctatc atcaaaatcg tacccatttt ctcatctttt
     8041 tctcgatata agtagcccat aataacagca acagcaccaa atacaacagg aataaatgct
     8101 aacgatacta ccatagaaat ccaaccaata atcaaataca gcttccctgt ctcttgtcca
     8161 tgtacgattt gttccttcat ttcattaatt gtctgctcag tacttttctt tactgtgtct
     8221 gttccggttg tctcatctgt ttcttgtagt gctccacaaa actggcaata cttactttca
     8281 tctgttattt ctttgccaca ttttctacaa tacatgtctt ctcctcctaa aatcattcaa
     8341 ttgcttatta gttaactgtt tactttatta aatacatcag aaaacagctc attcattggt
     8401 acacttaata tctcagaaag catcttcatt tgatagggtt taggaatcgt tctccctgtt
     8461 tgccacgaac ttacacattg ctgtgtaaca ttcatttttt ttgctaactc cgtttgagaa
     8521 agtccttttt cttttctgta tcggctgaga ttacttactt ttttcatgat tctcccctcc
     8581 tgttttatta tatacaacaa tatgatgtat ttcaactcat aaaatataaa aaacttcaaa
     8641 taccattaca aatacaacaa cttgttgtac tatactgaga tgggggtgta ttaatgaaac
     8701 agaaacaata tgcaaaggtc attagagaaa agcgaaaagc tctcgggtgg acacaaaaag
     8761 aattagctga aaaaatcttc tctacacaac aggcagtagc aagatgggaa aattctgtaa
     8821 cagagcctaa cctcgatagc ttaacagctc tttctagagc cctaggaacc cctgtaagcc
     8881 attttctaga caatgttgtc gtagattatg aggaagagtt tttggctctc tatcgttctc
     8941 ttagcactga agatgcagtt cgaacaattg actatatgaa gctattaaaa aggcaagaaa
     9001 atgaacgcaa tcagctattg aaagattgaa aaataaataa cccaccgaac tttaacggtg
     9061 ggtgtattga gatgaaataa gattaagagc tatggataac ctgtctagtt taatacaatt
     9121 gggtcttttc tcttccttac tctccgtcca tcatctcttg ttacacttgt aaattgatac
     9181 agaggaacta tcccagctcc tgataatatc aattctttta ttgtttcatc tatccctgcc
     9241 cctttcgcta aatatattaa aaaactacta cttgctttca cattgtatag tttcttaata
     9301 tccttagaag ctcgttcaag agtgaatcct tgtttaacaa agtagcctct gttttttatt
     9361 aaagcatttt tcacgctgtt atccccaaca ttacgtaggc gttcacaaac agtgttaata
     9421 agttctttct ctgtcattag atatgtccct tgccacgata agccctgtaa aacttcatgt
     9481 gcttcatcta aaatcaattg tttttccttg ctgagttctg atcgggaatt ttgaggaaat
     9541 actcttttag cctctttttc gttaaaacat atcttaaccc attcataaga aaaatattga
     9601 tatgtaaaat gaattttttc cagaagtgga aggatatctt cattcaattt ttgaatcaca
     9661 gaagttgcat taaaaacgtc taatcgatag acattgcttc tcttatgatt aaagtttcct
     9721 aacgctttct cttcctttaa cctatttagg tattcgagca tcttcaaagg aacatcttca
     9781 tcatctaatt tttttatcaa tcctataaaa gtcataagtg atagtaactt attacattta
     9841 tctatgtcat aagttttacc atagtaagaa ctcatttttt tagctaattg ttcccccgac
     9901 aaaatacaag tcacactttc cttactatca tcaaaaaaat taacatcaga aagcaagaca
     9961 tcatagacac gtcgcatttc ctctagataa ttccttattg tcctgaaaag tttgtctttc
    10021 attgattcaa gagtgtcaaa aagtactttt gattcctctt tcaattttcg aaaatgtgta
    10081 ggatacaagt ctaggttctc taatagccaa ttaaatgttt ctacccgact gtctttcttt
    10141 aaaattagca tcacaactcc aataaaattt tcactgatat cacaattact ggaatgacaa
    10201 taaaaaatgg aatgcccagt tttactggta aaaatactag cagagggatt atgatcgttg
    10261 tgaaacagac atcttaagtt tttacttgac ttaagtaatt tattcatatc aatggttaac
    10321 aatctttcat aaatctcatt aaaagacatg tcggaggaat caaacaggca gtctttaaac
    10381 cactgtttca tttcttcttt gtcgtcattt tttatcaact taacaaaatc ggtttcattg
    10441 actagttcat actctctccc tgaatcactt tgacaatttt tagaacatgt catggtaaaa
    10501 tcaatcggaa gctcagtaat atctaacatg ttgtcttcat taattattat cactgcatct
    10561 gacttatatc ctccaaaaaa cattctattc gaagcattta cattgtcatc acacccgggg
    10621 atgttctttt tcaagtattc gtaaacagca accattttac catagtcagt aattgcttct
    10681 tttaatagaa agacaatctt gtatttttct agattttcag agtaccgaat acttttttgt
    10741 atcgcaaacg cagaattatg aactaaattg atagataaaa ccttttctac gcttaagtat
    10801 ctactatcat ctttactcaa atctactacc ttccccttct catctttaac agtgttatcg
    10861 acatccagaa taattactct ggtttccaca aaactctcct tgtcattgcc tttattcttt
    10921 gtcttgcttg gaataaatga ttgtgatgaa tttagtatct ctacaaactc atctcttgta
    10981 acttctctat agttatctaa acacctatct cttacatcag ttttttcttt ttttgtaact
    11041 ggcttctcta ataatttttc ttgatctaca taaattggaa tattaatcat tgatgtttcc
    11101 atttgtgttt cctccttata aaattctgtt aaagactaaa acatacagat aataaaaaca
    11161 aactctttat tactatatgt tttagtctta tttgtttact caacctttat accgttccat
    11221 gaaactatca atatcagatt ttttaaatct aatgaccgta tcaattttta taacaggtaa
    11281 cccgtattga gaaacatatt ttctaatcgt tcctactgat acacctagat attcacttgc
    11341 ctgctgatag tccatatact ctagagtgac tccagaaata atgtcttcta gcataggctt
    11401 tagcactgta attatctctt tcacttgttg ttcaacttgt atagaaacaa tgctatcaat
    11461 gatttgtaga gacatatttc cccctccttt cgtgattgct tgaacaaatt ttctaagaat
    11521 tatcttcgta ttttttgctc aaaataaaag tattttgtta ttaaccgatt aatcaaacaa
    11581 aaaatgatta ccagttcatc gacatacaca taatacctcg ataaaaaagg ggtgtcaaca
    11641 gctttgtaaa atttatttgc aacacccctt ttttgaggta tactaatttt aggaggtgtg
    11701 gtcaatatga taaaacgtcc accaataaat tatttagaac gaaaaaagat actcggaaca
    11761 aaaataaaag ctataagaaa gagtaaaaaa ttaacacaac cagcatttgg tcttatgatt
    11821 aacaacggtc aattaattga caaaaaaact atctacgaat gggagaaggg aacctattta
    11881 ccaataccag agcgattatc ccgaattgct gatttgggaa atatgagtat agaggagtta
    11941 gtttgtggca atgtagaaga atatattttg ggaattattc tttatagaga tagcattgta
    12001 ctagatggta taacatttcc agataaaaac ctgttccagc atcttcgtca acagttccca
    12061 ccagtacact ctaatttaga tacatggctg gatagatatt ccaaattaga accagagatg
    12121 caagaattta ttgctaacaa gacttgtaat aaagttaaaa atgaaaaaat tagtcttttc
    12181 aatatcttaa aaatagaaga attattcatc aatgctattg tagaagaatt tgacaataac
    12241 attcttttcc tcactagcag tatagaagaa ctactagaaa gaatggtaga tgaatggcta
    12301 cctattcaat tgaaagacat gtcttatccc gaagaagctg tgagagaaat tactgataat
    12361 ataaacaagc tagaacaaac gatttcatca attgggaaaa aatacactaa gaaaaaaatg
    12421 aagggaggtg ataccatcta gtggtaagta ctccttgtaa actaagagga gatgatttaa
    12481 tggcaaaaat atcaaagtac aaaaagaaaa acggtcaagt ggcgtggatg tataaaggac
    12541 acattgccac cgatccacga actggtgaaa aaataaatac tacgagaaga ggttttagta
    12601 gcaaacaaga agctcaaaaa gattatgacg agtacagaca tcaaatatta tacggtgtta
    12661 aaaagaaagc acctgatatg acatttgaag acctgtataa tgaatggata actcatcagc
    12721 gtaccagtgt taaagcttca acaatcgcaa tctcagtaag atacgcaaat aatcaaatac
    12781 ttccagcatt tggtaagctt aaactatcga atatctctgt cccatattgt caaaaagtgg
    12841 tagatgaatg gcacagtaaa tatgaaagtt acgattatat gagaaaacag actgcacaaa
    12901 ttttacgcta cggggttgca atgcaatata ttgacaataa tcctatggaa aaaacacttc
    12961 ttccacgaaa aaaagagtat gagaaaaatc gtaaatttta ctcaaaagag gagcttaata
    13021 atttactgga tgcttttaaa gattttggta acatgaaaca atacgccttc ttcagattat
    13081 tggcatacac aggtatgaga aagagtgaag tactcgcttt acagtggaaa gacatagaca
    13141 cgtttaataa agaactacat gtcaataaaa ctcttgctgt agatgaattt ggtaaagtta
    13201 taattcaatc tccgaaaaca agagcttcta gacgagttat ctctttagat actgagactc
    13261 tttctatcct caacaactgg aaattacagc aaaaagaaga gtatttaaaa cttggataca
    13321 atactagctc taaagagcaa catgttttta caacagttaa aaacactcta tacattccaa
    13381 acactgttaa tgattggtta agatatattt tgaaaaaata taacttgcct cgtattacac
    13441 ctcatgggtt tagacataca catgctagtt tattattaga agcaggagaa tctgtcaagg
    13501 tagttcaaca acgattaggg catgaaaata gtaaagtgac attagacatc tatgcacata
    13561 ttacaaataa tgcacctaag aaaacaggtc aagactttgc tgatatgatg gctcatcagt
    13621 aaaagagctg gtcaaaatct ggtcatttct agtatacagg caaaacaaaa aagctagaac
    13681 ccttatatat caagggttct agctcctata acaggtttac ccaacactgc cttccatctc
    13741 ataactgatc aatcgattga gttcgactgc atattccatt ggaagttctt tggtgaatgg
    13801 ttcaacaaag cccatgacga tcatttcagt tgcttcagat tcggtcaggc ctcggctcat
    13861 cagatagtag agttgttctt ctgagatttt tgagactttg gcttcgtgtt ctaaagagac
    13921 ttggctgttg tggatctcat tgaatgggat cgtatcggat ttcgaccatt cgtccatgat
    13981 gatcgtgtca cattcgatat gagagatcga acccgcactg tttttggcaa aggttacttg
    14041 tcctcgatag tttacttctc cgccgtcttt tgaaattgat ttagagacga tagagctaga
    14101 tgtatttggt gcattgtgga tcatttttgc acccgtatct tggatttggt ttgctcctgc
    14161 aaaggcgatg gagagcatgg tgcctcgtgc accttttcca tctaagtaaa cacttgggta
    14221 tttcatcgtt gtttttgcac ctaggtttcc gtcgatccat tcaacggttg cgccttcgta
    14281 tgcttttgct cgtttggtta ctaagttgta cacattgtct gaccagtttt ggatcgttgt
    14341 gtaacggcaa tacgcatctt ttcttgtaaa gatttctacg atggcggcat gcaggctgtt
    14401 acttgaataa gtaggtgccg tacaaccttc gacatagtgg atgcttgctc cttcgtcaac
    14461 aatgatcagc gtacgttcga attgccccgt gttttccgca ttgatccgga agtacgtttg
    14521 taatggaaca tcgactttga cgccttttgg cacgtaaata aaggtcccac ctgaccatac
    14581 ggctgaattc agcgctgcta atttgttgtc ggttggcggt acaagttttg aaaagtactc
    14641 tttgaataat tcagggtatt ctttcaaagc agagtcagtg tcagtaaaga cgattcctaa
    14701 cttttcgaat tcatctttca tattatggta cacgacttct gattcatatt gtgcggaagc
    14761 accggctaga taagctcgtt cagcttctgg tataccaatc ttttcaaatg tatctttgat
    14821 tttttctggt acgtcttccc agtcacgagc gggtttgtca cttggttttt ggtaatattt
    14881 gatggcatcg aaatcgatgt ctgaaaggtc ggggccccat gtttgcattg gcattttgtt
    14941 aaatgtttct agagatttca gacggaattc aagcatccat tctggttcgc ctttgattct
    15001 tgacatctca cggatcactt cttctgtcaa gccttctcct gtactgaaaa ccggttcgac
    15061 atcatcgtga aacccgaatt tatattcttc taattctggt acgcccatgt tctcactcct
    15121 cttccttgtg gtaatgatgg aaagctgcgg taccatcgtt ttccattgct tttcctagtg
    15181 ctttccaggc aagtgtcgca catttgatcc gagcgggaaa tttagctaca ccaccaagca
    15241 tagcagcatc gcctaattgg tcgggatctt tgacttcttt tccttggaca agctgtagaa
    15301 agtctacagt taaatgttct gcttcttcta ttgattttcc gatgacggcg tctgtcatca
    15361 tcgaggcact tgccgtactg atggagcagc cactgccgtc aaaggcgata tttttgatga
    15421 ttccgtcttt tacatcgact tgcaattcga tcacatctcc acatgtgggg ttgttcaatt
    15481 cgatcttgct tgtcgagtca gtcaagcttc cacgatgatg cggatgggaa gaatgatcca
    15541 agatcacttg acggtataaa ttgtctaatt tagatagtcc catgttggaa aaactccttt
    15601 gttgccatga ttgcttcaac taaacggtcg gcgtcttgcg ttgtgttgta gaaataaaag
    15661 ctcgcgcgag cagtcgctga tacttcaagg tacttcaata gcggttgcgc acaatgatga
    15721 cctgctctga cggctacccc ttccatgtct aaagctgtcg cgacatcatg gggatgtaac
    15781 ccatccaaat tgaatgcaac gactcctgtc cgaaaattcg ggtcttgtgg accataaaca
    15841 gtcaatccat caattgcttg taattttggc aacacataag cggctagttc ttcttcatat
    15901 cgatggatat tgtccatacc gagtttttgc aaataatcga ttgcatggcc taatgcgata
    15961 gcgccggcga tatttggtgt ccctgcttcg aatttccaag gtagttcttt ccatgtactg
    16021 tcgtataatt cgacaaaatc gatcatttcg ccaccaaatt caacaggttc catttcttct
    16081 aaccattttc gtttaccata taaaacacca atacctgttg gtccgcacat tttgtgtccg
    16141 ctgaaagcaa agaaatcaca gtctagtgct tggacatcaa tggccatgtg cggggcagat
    16201 tgagcaccat ctactacgat gaccccgccg ttttcatgga caagttcagc taattcttga
    16261 acaggattga agacacctaa gacattggat acatgagcaa tggagacgat tttggttttg
    16321 tccgtaatct ttttcgctgc atcctccata tccaggaagc cttcttctgt tagttcgata
    16381 tatttcaaga cagcacctgt tcgttttgcc aactgctgcc acgggataac attagaatga
    16441 tgttccatgt aagagagcac tatctcgtct cctgctttga tatagcggtc gccgaaggtt
    16501 ctggctaccc aattcaagct tgtcgtcgta ccacgagtaa acagcacttc tgctgtttct
    16561 tttgcatgga tgaacgcccg tactttttct cttgcttgtt cataatcatg ggtcgcgcgt
    16621 tctgctaacg tatggacacc gcgatgaaca ttggcgttat cgtgttcgta atagtacgct
    16681 aatgcctgga gtacttgttt tggcttttgg gtcgtcgctg cattatccag ataaactaaa
    16741 ggttcatcat tgacgtcttg aaataagata ggaaaatcat tgcgtagctt tttagcatcg
    16801 atcatccatt caacttccct tcgattactt cgactaattc tttccgaact tgttctacag
    16861 ggattgctgt taagacagaa ccgaggaatc ctcgaatcac taagcgctct gcttcttctt
    16921 tatgcaatcc tctactcatc agatagtaca tttcttctgg atcaactcgt cctacactgg
    16981 cagcatgtcc agcagttact tcgttttcat cgatcaaaag aataggattc gcatcaccac
    17041 gtgctttatc agaaagcatc aagacacgac tttcttgttg ggcatctgca ccttttgccc
    17101 ctttcaagat gtggccgata ccattaaagg tcaatgttcc tttttctcgg atcactccgt
    17161 gttgcaaaat atgcccgatc gtatgagggg ctttattggt tactcgtgta tcgattcctt
    17221 ggatttgtct gcccgagctg attgccacga ttttgacttc tgcgtgtgca ccttctcctg
    17281 ctaaatctga gtcaaagtca gcgatcacat gaccgtcatt catgacacca attgcccagt
    17341 cgacagaagc atctcgtaaa atgtgcccac ggcgattcat ataagaagta atattttctc
    17401 ctaattgatc tacggctgaa tatttgattt ttgaaccagc tttagcgatg acttccacga
    17461 tgatattacc tgatgatttt gctacttgtt ctttggttgt ctggaaacgt tccagataag
    17521 aaaattcact atgttcatct gccacgatca acacatgctt gaagaagtgc tcatcggatg
    17581 cgccatcttg aatgaataag gactcgagtg gttcatcgat cacgacattt ttaggcacat
    17641 acaggaaaat accgctgttc ataaaagcta catggagtgc agtcaattgg tcttcgttca
    17701 tgtttactgc ttttttcata taatattctt ggactagttc tggatattcg atcattgcag
    17761 tgaacaagtc ggtaaaaatg actccttttt ctgctaattc cacaggcagt tgttcaaaaa
    17821 tcgtccatga accttgttgg acaagcacag gattgtcttt cattgcatca aatgccggaa
    17881 tcgtgcctgt ttcactgatg gtttgtgttt ctttcacatc aaataacgac cagcgatgga
    17941 atttcacccg atcgatatgc ggcaatggca gttcatctgc tttcgctagt gcgtcttgac
    18001 ggaaggttct catccaatca ggttcgcctt tacttaaaga aaaatcattt accgcgtcaa
    18061 gataatcgag ccagtttttt tctttcattc ttgctccccc ttacgcttct tcttctttat
    18121 agtcgattcc taattcttta ctgataccag cgtagccttc tgcttccaga cgtttagcta
    18181 ggtcagcatt ccctgtcatg actactcggc cttccatcat gatgtgaacg acatcaggtg
    18241 tgatatagtt caataatcgt tgatagtggg tgatgatcaa tgcaccgaaa ttgtctccgc
    18301 gcatttcatt tactccttta gcaacgactt tcaaagcatc gatatccaga cctgaatcga
    18361 tttcgtctaa aattgcaaat gtaggttcta acatcaacaa ttgcaggatc tcattccgtt
    18421 tcttttctcc gccagaaaaa ccttcgttca gataacgttc agccatttct tctggcatat
    18481 ttagtaaagc cattttttca tctaactttt tcaaaaatgc cataacagaa attttgtcgt
    18541 tttcatcacg tttcgcattg atcgctgcac gcatgaattc cgcattcgtg atacctggta
    18601 tctcacttgg atattgcata gctaaaaata agccaagtcg cgcacgttca tctacttcca
    18661 agtctaaaac atttttcccg tcaaacaaaa tttcgccttc tgttacttca tagtttggat
    18721 tgcccatgat agcggcagat aaggtagatt ttcctgtgcc gttaggtccc atgatggcat
    18781 ggatttctcc ggttttcatt gttaggttta cgcctttaag gatttctttt tcctcgatcg
    18841 acacatgtag attttttatt tctaagacag acatgcatta tacccccaaa ttctctttcg
    18901 ttctcactca ttttagacta attgagaatg aagcgcaatg accttcctta ctaaaatcaa
    18961 atatctttta tttattatta aacaattttg atttctaaat tagaataggt ataaaaaaga
    19021 tgagaaataa gccattacag acttatttct catctttttt gttccatacc ttattttgca
    19081 tcagccttta ctggtttaac agagccgccc catttgtctg tgatccattc ttggacttct
    19141 ttgcggtgaa gcacttcaat cagttttttg attttgtcac tgtcttcatc acctttacga
    19201 gtcgcaataa tattcacgta aggagagttg tccttttcaa gcaatacggc atcttctaat
    19261 ggattcaacc ctgcgccgta agcgaagttt gcgttgattg caactaggtc accttcatca
    19321 ttttggtacg tagaagctaa aagtgatggg tcgatcgtat ggttgaactt caagttctta
    19381 gggttttctt caatatcatc gaatgttgct gtttccagat ctactccatc tttgactttg
    19441 actaagccag catcttgtag aattgtgatg atgcgtcccc agtctgattc agaatttgaa
    19501 gtaatgattg ttgcaccatc ttttaattcg gaaacatctt tgactttttt agagtacaag
    19561 cccattggtt cgatgtggac agcaccggca ttgacaaagt catatccttt ttctttgatt
    19621 tctttgttca aataaggaat atgctggaag taattggcat caatgtcgcc ttcttccaaa
    19681 gattggttcg gtaatacata gtcatcgaat ttgacgattt caagatcgat tccttcatct
    19741 ttcaataatg gtttcacatg ttccaaaatc tctgcgtgcg gtgtcggtga agcgccgact
    19801 ttcaatgtcg tgtcgtctgc tttatttgct gaatctttgc tgtcgctgtt gttaccgcat
    19861 gctgctaatc ctacagtcag caaaagcgct gctgcaaatc caaaaatttt ctttttcata
    19921 tcctacatcc ccctatatcc tattatcttt tgtctgtttt cttagttaag aaatcaccga
    19981 ttccttgaac gataaatacg attagtaaaa taattaaagt cgcaacgaat gtcactgtta
    20041 gattaccacg ctggtagcct tcctgccaag caagacctcc aagtccacct gcgccgatcg
    20101 cgccggccat cgcagtaaat ccgatcatcg agatcgttgt aactgttaat ccagaaatca
    20161 atgcgggaat actttcaggt atcaagacct tccagatgat ttcccagtaa cttgctccca
    20221 ttgcatctgc tgcttccagt acaccagggc tgacttcacg aaaagcaatc tctactagac
    20281 gggcataaaa tggtgctgcg gataaaacaa gagctggaat ggctgcttgc gcacctagca
    20341 tagtgcctac gattgcttta gtaaatggaa tgatcaaaac catcaggatc ataaatggcg
    20401 ttgaccggaa aatattactg atgatcgata cgattccata aatgattcga gcatacggtt
    20461 tgtttttgcg tccaatagag taaaggatca atcccaaaac taatccgatg atcgtcacta
    20521 agaccattga gattaatgtc atatatagtg tatcaaggat agcctgattc atcgtttccg
    20581 ggttgatttg tttaaaatct agatatgttt ctgaaaagct tttatccacg atggatcacc
    20641 tcaattccta ctccttgttc tttgatgaac acttctgctt gatctaattc tttggtttca
    20701 ccagtcatca agacagtcaa tgaaccaaag gaaccttctt tcacttgttt gatctttcca
    20761 tacacaacgt tgacatcgat gttatagcgt cgaatcgctt gggaaatgac tggctcgttg
    20821 gcatttcttt cgctaaaggt caaagttgcc aaacggccgc ttggattttc tttgatgagt
    20881 tcagctaata gttctgtcga atcagcttct ggttcgatgt cttgttgtac aaagcgtttg
    20941 gttacttctt ttttcggttg tctgaagaca gtcaatacgt ctccttcttc tacgactttc
    21001 cccaactcca tcacagcgac tttgttgcag attttgcgga tcacattcat ttcatgtgtg
    21061 atcaagacga tggtcaaatt caatttctta ttgatcgcta acaataagtc taatacttct
    21121 tctgttgttt gcgggtccaa tgcgctagta gcctcatcac aaagcaggat ctcaggatca
    21181 tttgccaatg ctcgtgcgat accgacgcgt tgtttctgtc cgccggaaag ttgtgaagga
    21241 tatgcttgtc cgcgtccttc taacccgact aaacgcaaca gttcttctgc ccgttcttta
    21301 cgtttgctct ttggtacgcc agctaattcc agcggcagct ggatattttc aagtacagtt
    21361 cttgaccata acaggttgaa atgctggaag atcataccga ttttctttcg gaatgtacgt
    21421 aattctttgt tttttaattt tgtaatatct tgatcgttta cgatcacttc gccgctcgtg
    21481 ggaagctcaa gtccattcag gagacggact aatgtacttt ttcctgcgcc agaatagccg
    21541 acgattccat aaatatcccc tttttcaacg tttagtgaaa cgtcattgac ggcttggatg
    21601 tttccgtttt tcccattgaa tgtctttttg acattcgtta gttggattaa tgacatgcca
    21661 ttcacatctt tctcttatga tttttcaaac aaaaaagcct tcgccctctt gccttaagca
    21721 aaaggacgaa agcttgaact tccgtgttac caccttgatt taccgttact tcgcaataac
    21781 agcctcttcc agtacttgga cttagcttct ttccgtatac tgtcgcgctg taacgggcac
    21841 tcccgtagta agtttgctta cgctcgctta ctctgctcta agaccatctt ccatctcatt
    21901 ttcgttaccc attttcagca aacagggctc tcttcaaacg tccgtgacat gtactcttct
    21961 tttcaaagct ttctcgtttc atataggatt gcttatgatt atagcaactg gaagaatcag
    22021 tgtcaacttt tttcaagtga ttttctgatt ttataattcg tcaaattctt ttggatcaac
    22081 atctttgaaa cttaagatcc acgcattcat ttcatctgaa tcattcaaga tattgatatc
    22141 ttcaccgatt ttatcattaa cagaagaaac tgtacccgtt agcgggctgg caaattcatt
    22201 gaccgctttt tccgcttcaa cttcgatcag aggttcatct ttttttagcg attgtcctgc
    22261 ttttggcaga ttggcaaacg tgatgtctcc aagttcttct tgcgcttcat ttgtcaaacc
    22321 aacacagtat tcttttccat taaataagat ccaaaggttg tcctttttct tcaaatgctt
    22381 gcctgccatt ttatatcagt tctccttcat aaattttttc attgaatcca attgcaacaa
    22441 gctggtcctc tcgtaagatc agtggtcgct tgatcagcat accatctgtc gataagactt
    22501 ggctcgcttt tgaagccgta aactcgccta cttgatcttt cagtccaagt tcccgatatt
    22561 tcatcccgct tgtgttgaac aggcgacgga ttggtacttg actcgtttct agccaagtct
    22621 caatcgttga ggccttcggt acttctttga tcatatcaac ggtttgataa ctaacgccat
    22681 gctggtctaa ccacgctttg gcttttttgc atgttgaaca tttcgggtac cagtaaaagg
    22741 tgtacaattt tcttacctcc ttgaagatct tgcaggtgct tcattttggt atctcatcga
    22801 taatatcaac ccgataccga tcatgttccc taaaatcgag gatcctccct gactaatgaa
    22861 aggcaatggg atccctgtta aaggaagtaa tccgatattt gctccaatat tctcaaagac
    22921 atggaacagc agcatcatga tcaaaccgct tgcgatatag gcgtaaaatt cattattcgt
    22981 atcgaaacac acccgaatca tacgatagat taaaataaaa tacaagaaaa tgacgaatgc
    23041 accaccgata aagccgaagt tttcaccgat aacggtaaaa atcatatcgg actcacgaac
    23101 aggcacatag atattgctta cgttgaatcc tttaccaaac aagccaccgg taccaattgc
    23161 caaaagtcct tgggccggct gataactcat tcctgacgta tcatggaaag ggtccagcca
    23221 tgaatcaata cgtgcaaact gataagattt gaatccaact ttataaagca agtctcttcc
    23281 gccttcagtc gtaaccaaga agatcgttcc tgctccaacg actactgcca agattactac
    23341 aggtgcgatg atgcgccaag agattccaga catcaggaaa atcccgccaa agatcgcaag
    23401 aaagacaagc atcgttccaa agtcatcttg ggccatgatc aatgctaaaa ccggtattgt
    23461 cacgatcaag agcttcccaa tcagccaaaa gtccgatttc atcgtccgtt cttttacttg
    23521 cgtattatgt ttagttacga tcagtgccat catcaagatg tacgcaatct tcatcaactc
    23581 tgctggctgg aaagtaaacg caccgaatcg aaaccagttt ttagaccctg ttgaagcagc
    23641 taaattctga tcatagaatt tgagcaagag tcccattacc actaacccca aaccataaag
    23701 gtaaggagtc agtttccaga tccatttgga attcaaatgc atgatgacaa cgattgctgc
    23761 cgctccaact aaataccaga caacttgcat ccctacgcct ttcaagactg ctggattgtt
    23821 cggatcatgg ctaagtgcta catataatga gagcagacca atcaaacata acagaaagac
    23881 tggtaaaatc acgccataat caatacgatt atcgttcgaa gcttttaatt ttttttccat
    23941 gtttcatccc ttttcttaaa aaccatactg ctgattatta tagcggtcca gttagataat
    24001 ggaaagtctt tccatagaca tttgggaaaa attaactttc cgtttgtgca gttgcttgag
    24061 caaatgcagt acaaaagcct actgcttatc tttagtgtat catagaactt tttttgatac
    24121 atgcaattat ctaccagctg agattctcct cgctgtgaga agtttctgtg ctttcattgt
    24181 atcgaaggat ctcttcggca atctctagca gctgatcaga aaccagattg gttcgttgga
    24241 tgaaataaaa atatcgatgg aaattcaacg gagtcattgg cacattttct ggaacagagc
    24301 gcttagacaa aatcgagcga ccgattcctt ccttcagcaa cgcaacaatt acatcgttac
    24361 ttttcacgat catcttctta tcttggatat tcatctcttc aaaataacgc tgcgtgtaat
    24421 gaaaaacacc agagtttgct tctcttacaa gccacaattt gctttttggg tccccagcga
    24481 tgactagctg gtcttccatc aaagagtatc tttgtacgcc ggaagtctct aatggctttt
    24541 cgataaaacc aaaatcggat tcatgtttcg ctacttgttc taatgattca tgagaattcg
    24601 tgatttcgat ctcaaaatgc actttaggaa accttttgat cagatgtttc atcaactgag
    24661 gcaaaagata gacagcaaag gtgtgggaag caacgatccg acatacttct tcttcatttt
    24721 gttcattgag caatttttgc tgagtttctt cccattcttc taacaaaacc agtgtccgtt
    24781 catataataa gtcggcttgc ttagttggaa gcatctcttt tcttccgtta cgttgaaaaa
    24841 gatcgatatt caattcccgc tccaactgtt tgatttgatt cgagactgct ggttgagata
    24901 aaaacagtac ttctgctgct ctggaaaaat ttcttgtttc atatactgct cgaaaagtcg
    24961 ttaataactt aaacatggca ccacttccat aactttttat gataattact ataaaaacta
    25021 tttgatttaa atatacaaaa aaccatctat aatagaaatg tacaacaaaa cagttgctga
    25081 tccaactagt aacttagatg aagacaatca gctgtctgct tgctcgtaat catacattcc
    25141 tattcatttt acaatgtatt cgttcatctt ggtactaaaa tctattaaat aaaaaggaga
    25201 atattatata tgaagaactt tttgaaaatt attccgatcc cgatctgcgg gttgatcctt
    25261 gggttagctt cccttggaaa tctgtttaaa gactatcacc atgttgcttt aggcaatcta
    25321 actggcggta tctctatgat tcttatgata ttcatttttg ttaaattgtt tatcttattc
    25381 gaacacacga aacaaacgtt gcatgatcca atcatcgcct ccgtttcacc aacttttacg
    25441 atgtcactca tggttatttg tacttatttt gtttcatatg aaagtattgc accaattgtc
    25501 aaattcattt ggctgtgcgc tgttatcttc caagtgatcc ttgtctttta tttcaattat
    25561 caccatgtta tcaaagctga tctttcaatt gaagcaattt atccaagctg gtttatcatt
    25621 tacgttggat ttggggtcat cactgtaact gccgggaact tcttcccact tattggaaaa
    25681 attttctttt ggatttcatt gatctgctat gcgttcttat tgccgatcat catcaaacgt
    25741 gtctttttcg taagaaatat ggctcatccg acacgtccgc tgattacgat cgttgcagca
    25801 cctggttctc tttgcctaac aggttatctt aaaaactttg ctgaacctaa tttttatctt
    25861 gtcgtagcat tgttcgcttt atctcaagta ttgtatttcg ttgtgctgtc tatgcttcca
    25921 cgtttgttca agcttcgttt ttatccaagc tatgcagcct ttacattccc gcttgttatc
    25981 tctgcaacag ctatatttac tactgttcat tacttcaaca gtttaggtat ctatagtagc
    26041 gtattggatt tcttaaaagt tgcggaatgt ctgtttgcaa gtgtagttgt cttctacgtt
    26101 cttgctcatt acgtacgttt cttaattaaa gaacatagca aacatggaaa atttgtttct
    26161 aaaaaaatcg cttaataatc gattcaatag gttgtgaaaa agctgttctg catcaagtaa
    26221 taagaacggc caaacaaaaa cagctgttca tgtttttgta ctggttgggc ttattaccgc
    26281 agatgcaagc ttttgaacac cgtttaccaa gaggtcgtaa
//
LOCUS       DACEEZ010000029        26292 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20313_2017
            SAMN12639966-rid9879223.denovo.029, whole genome shotgun sequence.
ACCESSION   DACEEZ010000029 DACEEZ010000000
VERSION     DACEEZ010000029.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639966
            Sequence Read Archive: SRR10023158
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 26292)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26292)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741683.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741683.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:44:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,016
            CDSs (total)                      :: 2,962
            Genes (coding)                    :: 2,889
            CDSs (with protein)               :: 2,889
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 36 of 73
            Pseudo Genes (incomplete)         :: 29 of 73
            Pseudo Genes (internal stop)      :: 18 of 73
            Pseudo Genes (multiple problems)  :: 8 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26292
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639966-rid9879223.denovo.029"
                     /isolate="E20313_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            185..1123
                     /locus_tag="IWO89_001294"
     CDS             185..1123
                     /locus_tag="IWO89_001294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein"
                     /protein_id="HAQ0456061.1"
                     /translation="MKKYLLTATVMIGALLFAACGNTNKEADKKEDLTIVTTFYPMYD
                     FTKEIVGDEGNVKLLIPAGTEPHDFEPSAKERAEISDADVFVYNSSDMEFFVDSLKDS
                     VDSKQTLMIEAAKGIDRLESQEADEHEESEEGHGHSHEYDPHVWLDPVLAIKEVRTIA
                     EELGEKYPDKKETFTKNADAYIKKLEALDQKYSDELKDATNRTFVTQHAAFAYLANQY
                     DLKQVAISGVSPDQEPTPSRLAELKEFVKKNNIKVIYFEENASSKVAETLSNETGVKL
                     EVLNPLESLTNEQIKAGENYISVMEKNLKALKESIN"
     gene            1146..1826
                     /locus_tag="IWO89_001295"
     CDS             1146..1826
                     /locus_tag="IWO89_001295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HAQ0456062.1"
                     /translation="MRYIEISDLTFYYDDEPVLEDVSYHVDAGEFVILTGENGAAKST
                     LVKASLGLLKPSKGMIKIAEKNQEGKKLSIGYIPQQVASFNAGFPSTVIELVRSGRYP
                     RGRWFKRLSEKDHQHVQRALEAVGMWDMRNRRIGELSGGQKQRISLARIFATDPDLFV
                     LDEPTTGMDEESRNEFYRLLRHSAHVHGKSVLMITHDHEDIKQYADRQIRLVRKEDSQ
                     WRCFHMNS"
     gene            1799..2623
                     /locus_tag="IWO89_001296"
     CDS             1799..2623
                     /locus_tag="IWO89_001296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HAQ0456063.1"
                     /translation="MALLSYEFMRRAFLAAIFIAGIAPMLGVFLVIRRQSLMADTLSH
                     VSLAGVALGFFLNLNPTLTTMLVVVLAAVILEYLRTIYRTYSEISIAILMAGGLALAL
                     VLMNLSGGNSATSIQSYLFGSIVTITWEQVIFLGILFVAIGILFFLFKRPMYVLTFDE
                     DTAHVDGLPVHLMSMMFNVITGVAIAVMIPIAGALLISAIMVLPAAIGMRLGKSFNVV
                     IAISVCVGFLGMLSGLTSSFYLDTPPGATITLVFIALFLIVNIIKRILVAFRRKNN"
     gene            2790..3608
                     /gene="purR"
                     /locus_tag="IWO89_001297"
     CDS             2790..3608
                     /gene="purR"
                     /locus_tag="IWO89_001297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pur operon repressor"
                     /protein_id="HAQ0456064.1"
                     /translation="MKVRRSERLVDMTQYLLNHPHELISLTSFAERYESAKSSISEDL
                     GIIKKTFKERGYGTLETIPGAAGGVLFIPEISYEEAKKYIESLAERLSEQDRLLPGGY
                     VYLSDLLGEPELLRQVGRIIASKYLGEKIDAVMTVATKGVPIAQAVSYYLNAPFVIVR
                     RDSKITEGSTVSVNYVSGSSERIEKMELSKRSLKRGSRVLVVDDFMKGGGTVNGMKSL
                     IEEFESELVGITVFAESKFNGHRAIDDYTSLLYVKDVDTQTKNITVVPGNYFSE"
     gene            4111..5484
                     /gene="glmU"
                     /locus_tag="IWO89_001298"
     CDS             4111..5484
                     /gene="glmU"
                     /locus_tag="IWO89_001298"
                     /EC_number="2.3.1.157"
                     /EC_number="2.7.7.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-N-acetylglucosamine
                     diphosphorylase/glucosamine-1-phosphate
                     N-acetyltransferase GlmU"
                     /protein_id="HAQ0456065.1"
                     /translation="MDARYAIILAAGKGTRMKSKLYKVLHPVSGKPMVEHIINRVSET
                     KPDEVITIVGHGAEQVKAQLGERSKYALQAEQLGTGHAVLQAASFLEGKKGTTLVISG
                     DTPLLTTETLNNLFEYHQGKNASATILTAQAENPTGYGRIIRDHIGIVEKIVEQKDAT
                     PEEALVQEINTGTYCFDNEALFDALSKVGTNNAQGEYYLTDIIEILKEEGHTVAAYQT
                     DDFEESMGVNDRIALAKANEIMRKRINQMHMVNGVSFVDSATTYIDAGVEIGPDTLIE
                     AGVQIQGNTVIGSDCVIGSHSKIVDSRIEDHVVIENSVIESSHVKKHADVGPYAHLRP
                     KAEIGENVHIGNFVEVKNAQIGKGTKVGHLTYVGDATLGEEINVGCGVVFVNYDGKNK
                     HHTTVGDHSFIGSSTNIIGPVEVAKNSSIAAGSTITDNIPEYALAIARARQVNKEGYA
                     KKLPYLN"
     gene            5578..6549
                     /locus_tag="IWO89_001299"
     CDS             5578..6549
                     /locus_tag="IWO89_001299"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016618611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HAQ0456066.1"
                     /translation="MSKHYFDPRLKIFALNSNRPLAEKIAAAVGVELGKSSVTQFSDG
                     EIQVNIEESIRGSHVYVIQSTSSPVNDNLMELLIMIDALKRASAKTINVVMPYYGYAR
                     QDRKARAREPITAKLVANMIQKAGATRMLTLDLHAVQIQGFFDIPVDHLMGAPLIANY
                     FLEKGIKGDDVVVVSPDHGGVTRARKLAEFLKSPIAIIDKRRPKANVAEVMNIIGQVK
                     GKTCVLIDDMIDTAGTITLAANALKEAGATSVYASCTHPVLSGPALQRIEDSAIERLV
                     VTDSIYLPEDRKIDKIDEVSVGGLMGDAIKRIHENKPVSPLFETKNK"
     regulatory      6555..6657
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            6723..7292
                     /gene="thiT"
                     /locus_tag="IWO89_001300"
     CDS             6723..7292
                     /gene="thiT"
                     /locus_tag="IWO89_001300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupled thiamine transporter ThiT"
                     /protein_id="HAQ0456067.1"
                     /translation="MGKNRVWVEGTIVAALAMVLSLIPIQIGSSFSISLGQIPLTLFA
                     LRRGTKAGMLAGFIWGILHFPLGQVYYLSVVQVLIEYPIAYTFAGAAGLMASNVQKGY
                     VDDNTKQIRKSIVFGALIGALSRYFWHFIAGVVFWGAYALWGMNPWLFSFVMNGASGL
                     STAIVTIIVLLGIQQTTPSIFVPDKRQEL"
     gene            complement(7373..8146)
                     /locus_tag="IWO89_001301"
     CDS             complement(7373..8146)
                     /locus_tag="IWO89_001301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0456068.1"
                     /translation="MTESVYVHIDTTSNAVLTKGLTATDFANSIVHFPQNLLLLDPSA
                     SAGEYESHTGLKVIRGTENIQRFFSSSRNRRGFDELKWIDFTDLTMLKELTPLEISEL
                     LYFGHMKTHLHSPFFYKLQNNFVYFDLNDQLNRIYYRYLDEFYRIFASKLSSILSDKI
                     NVKKSFFKKETPVEKISVELLKEMRNLMQEGIIFSFDQVGLIDGKYRIPIYVIEDYIW
                     KLKGTRYREEEIIATLIYSVTDRHWQLVEDADLSYLPLK"
     gene            8413..10113
                     /locus_tag="IWO89_001302"
     CDS             8413..10113
                     /locus_tag="IWO89_001302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HAQ0456069.1"
                     /translation="MEIKKVYFSQKMSLYSPIQQAKQPLPFFQSHQESRDAFWRSAKK
                     QCAFKAVSKKYTVYFTIAKSRGDVIFNNLLIEGETFEWKKFFRYMEACARFFIKENCC
                     FLFQSPLSEEWLRIFHKNGYQGTTQLNKKLVYHTALVLGGGGAHGAYQIGVWQALKEH
                     DINFEIITGTSVGALNGALILQGDMKKAVNLWKKLSTRQVLALPEMAAVEDLRERFYR
                     ERRQMTKTALIEGGVSSAPLEKMVKDNLDLSLLKHNPKIRLYTVSTKLPELAEVVTDI
                     QQTKTEEIPDWILASASFYPAMAYRKIGKNKYADGGYRNKIPIDIAINKGATEAFVVD
                     VQGPGPAKKIRMPDIFIHWKCQTLWTLGSFLLFDSQRNQLNLQLGYLEMKKRLGYYYG
                     NWYTFHSVKPARTYWRGFLSYLTKEIQLELSFFKSIKFWQKLRNLYKNRVVPETCGLA
                     MLELLAKKHFLLPNEIYQVDMMIQMICQQDIKSMPDDLLAQIGQLSTEEWRRYRKYQQ
                     KIQNERTKTVYFDYLLQGKRKDRLQHELLKQPVDTLLILYLYYLKEEQPWHKNFHMKS
                     "
     gene            10083..10301
                     /locus_tag="IWO89_001303"
     CDS             10083..10301
                     /locus_tag="IWO89_001303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0456070.1"
                     /translation="MAQEFSYEIVEEIAILSENNKGWRKELNLVSWNGRPPKFDLRDW
                     APDHEKMGKGLTLTNEEFEQLQKAIENM"
     gene            10321..11211
                     /locus_tag="IWO89_001304"
     CDS             10321..11211
                     /locus_tag="IWO89_001304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YicC family protein"
                     /protein_id="HAQ0456071.1"
                     /translation="MKSMTGFGKAIRETKAYQLDIEIKSVNQRFLDVQIRSPKILNFV
                     ENDFRQLIKQQLSRGRIEVFVNLAYIGKNEKQIVINWELIDSLITQLTDGIQQRYGSQ
                     NQVSISRMAERFVLDENFVTVEEKPEESMEELAQLALDTLKSALDSIEASREKEGQAL
                     ALVLKKNTEEFKEVLKKLDSFVEIYEKEFQIRYQKKLEEFLGAKVDEQRLLTELAILL
                     ERGDIHEELDRLAIHVQKLDELIEAQCPVGRELDFLIQEMNREINTIGSKSNAIEIKN
                     QVVQSKTILEKIREQVQNIE"
     gene            11229..11513
                     /locus_tag="IWO89_001305"
     CDS             11229..11513
                     /locus_tag="IWO89_001305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0456072.1"
                     /translation="MKYYDVTFHELSGKTVVKRDIPSEKNGFDVWKDACADYNENELF
                     ILINDGAYVTMNRKFIVRIDTEEVEDPTEKARSRKDEIMGVVNTLSNMGF"
     gene            12000..12614
                     /gene="gmk"
                     /locus_tag="IWO89_001306"
     CDS             12000..12614
                     /gene="gmk"
                     /locus_tag="IWO89_001306"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010766465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HAQ0456073.1"
                     /translation="MSERGLLIVLSGPSGVGKGTVRKAIFESEDNDFQYSISMTTRKM
                     REGEVEGVDYFFRSKEEFESMIEAGEMLEYAEYVGNYYGTPLSYVNKTLDEGKDVFLE
                     IEVQGAKQVKEKVPDGVFIFLTPPDLAELKSRIVGRGTDADEVIEKRMKVAKEEIEMM
                     ALYDYAVVNDEVPLAVERIKNIIASEHFRVDRVIGKYIKMLKEM"
     gene            12619..12924
                     /locus_tag="IWO89_001307"
     CDS             12619..12924
                     /locus_tag="IWO89_001307"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HAQ0456074.1"
                     /translation="MMLKPSIDSLLDRVNSKYSLVILASKRAHELDASAQPTLDSFDS
                     VKSVGQALEEIDAGNVVNDPHPELKRERLKMEEEERQAQKEREQHELESRIREEQKM"
     gene            13177..15585
                     /gene="priA"
                     /locus_tag="IWO89_001308"
     CDS             13177..15585
                     /gene="priA"
                     /locus_tag="IWO89_001308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HAQ0456075.1"
                     /translation="MTLTAEVIVDVPTMQTDQPFTYLVPSEMETALQVGMRVEVPFGN
                     GNRHVQGFVMAIQKSEESANPSLKAVIRLLDLAPVVNEELLSLADYMKKITYAFKITC
                     LQTMLPSVMKAEYDKLIYPLADTPEVQVLFDQREVISWKEAEEEGSLSQLIRWRQEQL
                     VDIKYEVHTRNKVKTIRLVRSLLTEKQIEEEWAKLRQNAKKQKELLLCLSELSQEEPI
                     AYFKNKGISTAVLNQGKEKGWLEFIESERYRDPYKDRVFDQTTALELNAEQKNAVEQI
                     ITAGQQQKDQVFLLEGITGSGKTEVYLQAIADVLSENKTAIMLVPEIALTPQMVERFK
                     GRFGESVAVLHSGLSQGEKYDEWRKIEREEAQVVVGARSAIFAPLKNIGLIIIDEEHE
                     SSYKQDETPRYHARDLAIWRSKYHHCPIVLGSATPSLESRARAQKGVYQLLQLNHRAK
                     AAAQLPAIEIVDMREEFQNHRTSTFSANLQEKIQNRLDKKEQTVLLLNRRGYSSFVMC
                     RDCGFVLPCPNCDISLTLHMDTRSMRCHYCGHEEPIPNRCPNCGGNKIRYYGTGTQKV
                     EEELRELYPQARILRMDVDTTRRKGAHEQILQKFGAKEADILLGTQMIAKGLDFPEVT
                     LVGVLNADTSLNLPDFRSSEHTFQLLTQVSGRAGRAEKAGEVVIQTFNPQHYAIELAK
                     KQNYEQFYQQEMHVRHRGGYPPYYFTVKITASHPEEQVAAKKIFQIANQLKEVLSPQS
                     LLLGPTPSMILRVKNRYYYQLIIKYKHEPNLPHVLDEILNGSQKEQRQGLFVAIDNEP
                     LNFI"
     gene            15600..16091
                     /gene="def"
                     /locus_tag="IWO89_001309"
     CDS             15600..16091
                     /gene="def"
                     /locus_tag="IWO89_001309"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HAQ0456076.1"
                     /translation="MRYPILIHPNDKLKRTAQPIDVITDETIALLDNLYETMIANDGI
                     GIAAPQVGQNKRIAVIEVDEGEKFELINPEIIEAKGESLDVEGCLSIPHVYGTVKRAD
                     EVTVRYYDRDGEEIEVTAFGYLARAFQHEIDHLDGILFIEKMIQQIPEEELEEYMEEH
                     LDD"
     gene            16084..17022
                     /locus_tag="IWO89_001310"
     CDS             16084..17022
                     /locus_tag="IWO89_001310"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HAQ0456077.1"
                     /translation="MTKLVFMGTPAFSVPILEGLIESGYEIQAVVTQPDRPVGRKRVI
                     TPTPVKKAALKHGIRVLQPEKISGSPEMEEIIELVPDVIVTAAFGQFLPEKLLQVPKL
                     GAINVHASLLPKYRGGAPVHYAIMNGEKETGVTIMEMIKKMDAGGIYAQESIPITKQD
                     DVGTMFEKLSLLGRKLLLETLPNILDGQKPVPQDESEATFSPNITREQEAIDWHKTAE
                     EIDNQVRGMRPWPIAFTTYEGIRWKLLSVEVLAETTDKAPGTIIRKDKKNLWIACGKQ
                     TVLAINELQPAGKGKQAVHDFLNGSGQQVQIGQQVK"
     gene            17022..18380
                     /gene="rsmB"
                     /locus_tag="IWO89_001311"
     CDS             17022..18380
                     /gene="rsmB"
                     /locus_tag="IWO89_001311"
                     /EC_number="2.1.1.176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(967)-C(5))-methyltransferase
                     RsmB"
                     /protein_id="HAQ0456078.1"
                     /translation="MKKIPKKMKKSVRYMALLTLERVNKGGAYSNLLLNEMIKQSELN
                     QKDVGLFTELIYGTISHQRLLEFYVAPLIAKAKKVDDWVKMLLYLSVYQLEFLDKVPA
                     HAILNEAVEIAKVKGNPGTGKFVNGVLRNYQRQGAPDLQGIADPIERLSVKISLPLWM
                     TKRLVDQIGYEETEKLGLSLYQPSHASARVDTRRLSRDQAITVLQEEEITAEKSQVSP
                     YGIVAKKGHLASSSLFHDGVMTIQDESSMLVAPSMQIEPEHHVLDACAAPGGKTTHIA
                     SFLDPSCGGEVVALDVHEHKVKLIEDNAARLGVSSAVYAQKMDAREVSKQFEAEQFDR
                     ILVDAPCSGLGLLRRKPDIRYKKQPDELANLPKIQLEILESCAPTLKSSGILTYSTCT
                     ILKEENQDVVSAFLERHPDFERIDVLTNPMLQSSIEEKMLTLYPHQFYTDGFFICCLR
                     KK"
     gene            18393..19133
                     /locus_tag="IWO89_001312"
     CDS             18393..19133
                     /locus_tag="IWO89_001312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Stp1/IreP family PP2C-type Ser/Thr phosphatase"
                     /protein_id="HAQ0456079.1"
                     /translation="MQIEYQSDVGRRRNTNQDYASVFTSRSGLKLAVLADGMGGHRAG
                     DIASQMAVTNLGEAWEKQEAVDDEKIAQWFIQSIQEENMLIYQRGQEQPEYNGMGTTI
                     VAAALSDDWFTIANVGDSRAYLIREDKIMQLTEDHSLVNELVKSGEISEEMAVNHPRK
                     NILTRSVGMPGTVEVDVSTHLWQLKDRLLLCSDGLTNMLSEEEIGTIINQEGPLSHKV
                     TELIDRANEAGGADNITVLLIEYKEDSV"
     gene            19130..21199
                     /gene="pknB"
                     /locus_tag="IWO89_001313"
     CDS             19130..21199
                     /gene="pknB"
                     /locus_tag="IWO89_001313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Stk1 family PASTA domain-containing Ser/Thr
                     kinase"
                     /protein_id="HAQ0456080.1"
                     /translation="MIELGKKLNGRYQITGNIGSGGMANVFLAHDLILDRDVAVKVLR
                     FDFQNDQTAIRRFQREALAATELVHPNIVSVYDVGEEDNMQYLVMEYVKGMDLKRYIQ
                     THYPVPYETAVNIMQQILSAISLAHNHQIIHRDLKPQNVLIDNEGVVKITDFGIAIAL
                     SETSITQTNTMLGSVHYLSPEQARGSMATKQSDIYALGIILYEMLTGSVPFDGESAVT
                     IALKHFQDDLPSIKALDPNVPQALENVILRATAKEPADRYKSAEEMSDDLSTVLSPAR
                     ANEEKWQPHVMDNETKVITPLTEDAPMPDSFKSMPLPKDKPENETLPEEEEVLPDPKK
                     KGQKKWWLILLAILAVLGIGTAVFFASGGRGEVVVPDVSDLSEASARETLAKAKLEVA
                     TKTEEIADDKIEEGNVVKTDPAAGTTVKQKREVTLYISSGTKKIKLDDYSGKSYEDAE
                     ASLKELGFSADLIQKEEEFSSEVESGMIISQSPSEDTEVDPKTDTITFTVSKGPETVT
                     LSSHVGENYETVRNWLVTQGFNINNISATYDYSDTVPSGSIISQDPASGEVVAEETYI
                     NFVVSQGTEPKLNDISGYTKSEAQSYLASVGAEYIGHETSEYSNTVDKDKVIRTVPGA
                     GTTITKGMVVNVIYSKGPDPASSSSEKDSSDSSSSSSSSSSTSNSSKEESSSSTSSSA
                     STSESSK"
     gene            21364..22263
                     /gene="rsgA"
                     /locus_tag="IWO89_001314"
     CDS             21364..22263
                     /gene="rsgA"
                     /locus_tag="IWO89_001314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome small subunit-dependent GTPase A"
                     /protein_id="HAQ0456081.1"
                     /translation="MALIKGQIRKAISGFYYVYANGETYQTRGRGNFRNRKITPLVGD
                     QVIFESENQTDGYLLEILPRKNQLVRPPVTNVDLGIIVTSIVEPAFSYNLLDRFLVTL
                     EYESIEPIIFLTKTDLSDDPEKIEEIKQTYTKIGYPVIVPNHTGDTTELIRYFPERLS
                     VFMGQSGAGKSTLLNQISPELNLATGEISDALGRGRHTTRHVELIPIYGGLVADTPGF
                     SSIDFLTIEAGELPRQFPEFVEASVHCKFRECMHAKEPGCEVKHRVETGGIAQTRYDN
                     YLQFLQEIEKRKPMYAKKEGKRK"
     gene            22276..22926
                     /locus_tag="IWO89_001315"
     CDS             22276..22926
                     /locus_tag="IWO89_001315"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HAQ0456082.1"
                     /translation="MKIAPSILSADFANLQRDIELVEKGGADYIHVDVMDGRFVPNIT
                     LGPNIVQAIRPVTKLPLDVHLMIVDPENYIPAFAKAGADIITVHVEATPHIHRALQMT
                     KSLGVKAGVVINPGTPVSMIKHVLPLADQVLVMTVNPGFGGQSFIEETVEKITELADL
                     RRENNWTYAIEVDGGIVPETAKICKDAGADVFVAGSYIYGAENPVGQIERLKEALD"
     gene            22927..23565
                     /locus_tag="IWO89_001316"
     CDS             22927..23565
                     /locus_tag="IWO89_001316"
                     /EC_number="2.7.6.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine diphosphokinase"
                     /protein_id="HAQ0456083.1"
                     /translation="MKILLAAGGNPQNWPQSLLSKYDAYVGIDRGALYLLQAGYPVDL
                     AVGDFDSLSNEEQKEVFAKAKEYTRSPAEKDDTDTQLGILKTFERYPQAEVTLIGATG
                     GRLDHLLANLWLGMEPRFKPFAHQLIIRDCQNYLSYYLPGEHIVRQIPDMKYLAYCCL
                     TPVSNLTLKGSKYVLENKEVEYPTSYASNEFLSDTAEFSFSDGMIAVIQSKD"
     gene            23720..24574
                     /gene="mreC"
                     /locus_tag="IWO89_001317"
     CDS             23720..24574
                     /gene="mreC"
                     /locus_tag="IWO89_001317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreC"
                     /protein_id="HAQ0456084.1"
                     /translation="MKKFNPNKNIIITLVIVIIVVTILSVTAAQRANEGKPNFFQSIV
                     NDSVAMVDKTISAPVRWVRNGVDSVHNLFSTYAENERLKEKIDSYDDVSQQNKNYERE
                     IEALKKELELNQTLTSYEKVTANVITRSPDSWQDMLIVDKGSKDGIEANMAVMSQKGL
                     IGRVIEVNTASSKIELLSSSNESSNHFPVRVSSANGEAFGLLKNYDEKLHALVVTQLT
                     GDTDIKEGDVVQTSGLGGNSPANLPIGTVIKTKPDSYGLDREVYVKPYAEMYDVSVVT
                     IVQRLAED"
     gene            24578..25087
                     /gene="mreD"
                     /locus_tag="IWO89_001318"
     CDS             24578..25087
                     /gene="mreD"
                     /locus_tag="IWO89_001318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreD"
                     /protein_id="HAQ0456085.1"
                     /translation="MLKKETMKYYLPIVLFFLMLIDGHLTRMLGEWSKGTYMSNAHFL
                     ILALLCCSMAFEKRYLLITTIVLGAIYDAYYIGVIGIYAVALPLIVWLMYVMKDVIHV
                     NIFTEFFSMIIFVTGYELFTMVVQLIFKLAVVNNTYFITRFLGPTLLLNMIIFVLFIF
                     PFKKLFSDE"
     gene            25342..>26292
                     /locus_tag="IWO89_001319"
     CDS             25342..>26292
                     /locus_tag="IWO89_001319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0456086.1"
                     /translation="MKKSLISAVMVCSMTLTAVASPIAAAADDFDSQIQQQDQKIADL
                     KNQQADAQSQIDALESQVSEINTQAQDLLAKQDTLRQESAQLVKDIADLQERIEKRED
                     TIQKQAREAQVSNTSSNYIDAVLNADSLADAIGRVQAMTTMVKANNDLMEQQKQDKKA
                     VEDKKAENDAKLKELAENQAALESQKGDLLSKQADLNVLKTSLAAEQATAEDKKADLN
                     RQKAEAEAEQARIREQQRLAEQARQQAAQEKAEKEAREQAEAEAQATQASSTAQSSAT
                     EESSATQSSMTEESSSATQSSATEESTTPESSTEESTAPES"
BASE COUNT         8788 a         4703 c         5656 g         7145 t
ORIGIN      
        1 tttttattgt agtgaaatgg ctagatatga gcagcaactc atccatttta cgatttaatc
       61 aagaatatac atgcttaaat cagaaattga tggatttcta ttgactgtgt ttttttattt
      121 tgatacatta gttcacgtta ataaatcgta aaattccgat ttgttcattg aaaggagcat
      181 ataaatgaaa aaatatcttt tgacagctac ggtaatgata ggtgctttac tgtttgcagc
      241 atgtggaaat acgaacaagg aagcagacaa aaaagaggat ttgacgatcg ttacgacttt
      301 ttatccgatg tatgatttta caaaagagat cgtaggggat gaaggaaacg tgaaactttt
      361 gatccctgct ggaacggagc cgcatgattt cgaaccaagt gcaaaagaaa gagcagaaat
      421 ttccgatgca gatgttttcg tgtataatag ttcagacatg gaattttttg tggattcgtt
      481 aaaggattca gtggatagca aacagacctt gatgatcgaa gcagctaaag gaatcgaccg
      541 tttagaatcg caagaagcag acgaacatga agaatcagaa gaagggcatg gtcattcaca
      601 tgaatacgat ccacatgttt ggctcgatcc ggtattagct ataaaagaag tcagaacgat
      661 tgctgaagaa ttaggcgaaa aatatccaga taaaaaggaa acttttacga agaatgccga
      721 tgcttatatc aaaaagcttg aagcactgga tcaaaaatac tccgatgagc taaaagatgc
      781 aacaaaccgc acatttgtca ctcagcatgc tgcttttgct tatttagcga atcaatacga
      841 cttaaaacaa gtagcgattt cgggcgtttc accagatcag gaacccacac caagtcgctt
      901 agctgagcta aaagaatttg tgaagaaaaa caacatcaaa gtgatttatt ttgaagaaaa
      961 tgcctcttcg aaggtagctg agacattgtc gaatgaaaca ggagtgaagc tagaagtgtt
     1021 gaatccttta gaaagcttga ctaatgaaca aatcaaagca ggcgaaaatt atatttctgt
     1081 catggaaaaa aatctgaaag ctttaaaaga aagtatcaat taagttttag aatggaggtt
     1141 gtaaggtgcg ttatatcgaa atatctgatt tgacctttta ctatgatgat gaacctgtac
     1201 tagaagatgt ctcttatcat gtagatgcgg gtgaatttgt cattttaact ggagaaaatg
     1261 gagcagccaa gtcaaccttg gtgaaagcat cgttaggatt actgaaacct tcaaaaggaa
     1321 tgataaagat tgctgagaaa aatcaagaag gaaaaaaatt aagtatcggt tatattcctc
     1381 aacaagttgc atctttcaat gcaggttttc ctagtacggt gatcgaatta gtccgttcag
     1441 gaagatatcc aagaggccgc tggttcaagc gtctctctga aaaagatcat caacatgtac
     1501 agcgtgcttt agaagctgta gggatgtggg atatgcgaaa tcggcgaata ggtgaattgt
     1561 ctggcggaca aaagcagcga atcagtttag cgcgtatttt tgctactgac cctgacttat
     1621 ttgttcttga tgaaccaaca acaggtatgg atgaagaatc cagaaatgaa ttttatcgtc
     1681 ttctccgaca tagtgcgcat gtccatggga aatcagttct aatgatcacc catgatcatg
     1741 aagatatcaa acagtatgcg gacagacaga tccgactggt gagaaaggag gattctcaat
     1801 ggcgttgctt tcatatgaat tcatgagaag agcgtttctt gccgctatct tcattgccgg
     1861 aatcgccccg atgctaggtg tgtttttagt gatcagaaga caatcgctga tggctgatac
     1921 gctttctcat gtctctttag caggggtagc gttaggattc tttttgaacc ttaatcctac
     1981 acttacgacc atgctagtcg ttgtattagc agctgtgata ttggagtatc tgcgaacgat
     2041 ctaccgaacc tattcagaaa tctccattgc cattttaatg gctggtggtt tggcgctagc
     2101 gttagtattg atgaatttga gcggagggaa ttcagcaaca agtatccaat cctatctgtt
     2161 tggttcgatc gtgaccatta catgggagca agtcatcttt ttaggaatct tattcgttgc
     2221 catcggtata ttgttctttt tattcaaacg gccgatgtat gttctgactt ttgatgaaga
     2281 taccgcccac gtggatgggc tacccgttca tttgatgtcg atgatgttca atgtgattac
     2341 tggtgtggct attgctgtga tgatcccgat tgccggtgca cttttgatat ccgcaatcat
     2401 ggttcttccc gcagcaatcg gtatgcgatt aggcaaaagc ttcaatgtcg tgattgctat
     2461 cagtgtttgt gtaggatttc tagggatgct gtcaggacta actagctcgt tttatctaga
     2521 tacacctcca ggagcaacaa tcacattagt ctttattgct ttgttcctga ttgtaaatat
     2581 catcaaacga atattggttg ccttcagaag aaaaaataac taaattataa agaaaagaag
     2641 ccttataaaa acgaaaaatt ttttataggc ttctttttat tttaataaat accgaactat
     2701 tttcttattt tttcataaaa tgctcgaatt tttgaagaaa aacttatata atgtaaaacg
     2761 ataaaatttc ttaaaaggag ttggaatagg tgaaagtacg tcgcagtgaa cgtctagttg
     2821 acatgacaca gtatttattg aatcacccgc atgaattgat ttccttaaca agctttgccg
     2881 aacgttatga atctgctaaa tcttctatca gtgaagattt gggaatcatc aagaaaacat
     2941 ttaaagaacg agggtatgga accttggaaa caatcccggg tgctgcaggt ggtgtattgt
     3001 tcatccctga aatttcatat gaagaggcga aaaaatatat tgaatctcta gcagaacgtc
     3061 tatcagaaca agaccgtctg cttccaggag gatatgttta tctctctgat ttgttaggag
     3121 aacctgaact gctgcgtcaa gttggaagaa tcattgcttc taaatacctt ggtgaaaaaa
     3181 tcgatgctgt aatgactgta gccacaaaag gcgtacctat tgcccaagct gtgtcctatt
     3241 atctgaatgc gccattcgtt attgtacgcc gggactcaaa aataacagaa ggatcaacag
     3301 taagtgtcaa ctatgtgtct ggatcttctg aaagaattga aaagatggaa ctgtctaaga
     3361 gaagtttgaa acgtggttca cgtgtccttg tagtggatga tttcatgaaa ggcggcggaa
     3421 cagtcaacgg aatgaagagc ttgattgaag aattcgaatc agaacttgtt ggcatcactg
     3481 tatttgccga atcaaaattc aacggtcatc gcgccatcga tgattatacg tcactgcttt
     3541 atgtcaaaga tgtagatact caaacgaaaa atattacggt tgtgccagga aattattttt
     3601 cagaataaga ggttgtgaaa aagccgctta gtttcgagta gtaagaaaaa ttttgtgaaa
     3661 atggctttcc acattttttc ataaatttgg cttaataccg agaaactggc ttttgaacac
     3721 cgtttatcta gaggttgtga aatcagcgtc ctgtcctgag tattagctca ataaacggaa
     3781 aaattgctcc tcatattttg ccgtttattg agctaatacc gaaggtcagc tgattgaaaa
     3841 acgtttaatg aagaggttgt aagcctaccg tttaactcta agtcacaaga aacgatttca
     3901 aaaaacagct tttcatgttt tatgagtagt gtgacttgtg accgaggagt tggcaggaga
     3961 gcaccggtta ccaaatagat aggcccgtta tggtctatct atttttttat ccagaaaacg
     4021 ctgggataaa agaaaatcac ttgttttgtt gaaaaaatgg gtatactggg ataaactgaa
     4081 atcagcaaac tatatttagg agtgacgcag ttggacgcac gttatgcaat tattttggcg
     4141 gcagggaaag gtacccgcat gaagtcaaaa ttatataaag ttttacatcc cgtttctggt
     4201 aaacctatgg tagaacatat cattaatcgg gtcagtgaaa cgaaaccaga tgaagtgatt
     4261 acgatcgttg gacacggagc agaacaagtt aaagcacaac taggagaacg cagtaaatat
     4321 gcgttacaag cagaacaatt gggaacagga catgctgttt tgcaagctgc ttctttcttg
     4381 gaaggaaaaa aaggaacaac gttagttatc agtggcgata caccattatt gactactgaa
     4441 acgctgaaca atctttttga atatcaccaa ggaaaaaatg caagtgcaac gatcttgacg
     4501 gcacaagcag aaaacccaac aggatatgga cgaatcatcc gtgaccatat tgggatcgta
     4561 gaaaaaatcg tggaacaaaa agatgcaaca ccagaagaag ctcttgtaca agaaatcaat
     4621 acaggaactt actgttttga caacgaagct ttgttcgatg cgttaagcaa agttggaaca
     4681 aacaatgctc aaggtgaata ctatctaaca gatatcatcg aaatcttaaa agaagaaggc
     4741 catacagttg ctgcttatca aacggatgat tttgaagaat caatgggagt caatgatcgg
     4801 atcgcgttag ctaaagcaaa tgagatcatg agaaagcgaa ttaaccagat gcatatggtc
     4861 aatggcgtaa gctttgtcga ttcagcaacg acatacatcg atgcaggcgt agaaatcggt
     4921 ccagacacat tgatcgaagc aggtgtacaa atccaaggaa atacagtcat tggttctgat
     4981 tgtgtcatcg ggtcacattc aaaaatcgta gacagtagga ttgaggatca cgtcgtcatt
     5041 gaaaactcag tcattgaaag cagccatgtc aagaaacatg cagatgtggg accttatgca
     5101 catttacgac caaaagctga aattggcgaa aacgtccata tcggaaactt tgtggaagtg
     5161 aaaaatgctc aaatcggcaa aggaactaaa gtcggtcact tgacttatgt tggtgatgct
     5221 acgctgggag aagaaatcaa tgtcggatgt ggtgttgtct tcgtcaacta tgatggaaag
     5281 aacaaacatc atacgactgt cggcgaccac agctttattg gttctagcac gaatatcatc
     5341 ggacctgtcg aggtagctaa aaacagttcg attgctgccg ggtcaacaat tacagacaat
     5401 attccagaat atgcgctggc aattgcgaga gcaagacaag tcaataaaga aggatatgca
     5461 aaaaaactcc cttatctaaa ctaagacttt tgcaaaatca cttgatttat ttaaaaaata
     5521 tgactactat ttattaggta gaattctaga gttgtgaaaa agaaagtgga ggtccccatg
     5581 tcaaaacatt actttgaccc aagattaaaa atttttgcgt taaattccaa tcgcccatta
     5641 gcagagaaaa ttgcagcagc agtcggcgtc gagttaggaa aatcatctgt tactcaattt
     5701 agtgatggag aaattcaagt aaacattgaa gaaagtattc gtggatcgca tgtctatgtt
     5761 attcaatcaa cgagcagccc agtcaatgat aacttgatgg agttgctgat catgattgac
     5821 gcactgaaac gagcaagtgc aaaaacaatc aacgtagtca tgccttatta tggctatgcg
     5881 cgtcaagacc gtaaagcacg tgcacgggaa ccaatcacag cgaaattagt agcaaacatg
     5941 attcaaaaag ctggtgcaac aagaatgctg actttggacc ttcatgcagt ccaaatccaa
     6001 ggtttctttg atattccagt cgatcatttg atgggtgcgc cacttattgc caattatttc
     6061 ttagaaaaag gaatcaaagg cgatgatgtt gtcgttgttt caccagacca tggtggtgtc
     6121 acacgggcac gtaaattagc ggaatttttg aaatctccga ttgcgatcat agacaaacgc
     6181 cgtccaaaag caaatgttgc agaagtaatg aatatcattg gacaagtcaa aggcaaaact
     6241 tgtgtactga tcgatgatat gatcgataca gcaggtacga tcacattggc tgccaatgct
     6301 ttgaaagaag ctggtgcaac tagtgtgtat gcttcatgta cacatccagt attatccggc
     6361 cctgctttac aaagaataga agattcagct atcgagcgtc tagttgttac agactcgatc
     6421 tatcttccag aagatcgtaa aatcgacaaa atcgatgaag taagtgttgg cggactaatg
     6481 ggagatgcaa tcaaacgtat ccatgaaaac aaaccagtta gtccattgtt tgaaacaaaa
     6541 aataaataat aaataaactc acaaagggga gtccaattgg gctgagattg aatctatttc
     6601 taaacccttc gtacctgtat cggttatgcg agcgtaggaa ttgtgaataa acagctgttt
     6661 gactattttc gaaagctgct tcctcctttg tgactttttt attcaaagga ggaagttttt
     6721 ttatgggaaa aaatagagtt tgggtagaag gaacgattgt tgcagcgtta gcgatggtgt
     6781 tgtcgttgat tccgatccag atcggcagca gtttttctat ttcattaggg cagattccat
     6841 tgactctttt tgcgttaaga agagggacaa aggcagggat gctggcaggt ttcatttggg
     6901 gcatcctaca ttttccgttg ggacaagttt attatttaag tgtcgttcaa gtgttgattg
     6961 agtacccgat tgcctatact tttgcaggcg cagcagggct catggcttca aatgtccaaa
     7021 aaggctatgt agacgataat acgaaacaaa ttagaaaaag catcgtgttt ggtgcattga
     7081 ttggcgcact ttccagatat ttttggcatt ttattgcagg agtggttttc tggggagcgt
     7141 atgcactatg ggggatgaat ccttggctct tttctttcgt aatgaatggt gccagcgggt
     7201 tgagtacagc tattgtaacg atcatcgttt tattagggat ccaacaaacg actccttcga
     7261 tttttgtgcc agataaacga caggagctgt aaataaaaaa gaagcaagaa acttgagaca
     7321 gaatcatgtc ctagtttctt gtttcctttt tattttttga taagaaatag atctacttca
     7381 atggcagata agaaagatcg gcatcttcta ccaattgcca gtgtcgatcg gtgacagagt
     7441 agattaaagt agcgatgatt tcctcttctc gataccgggt gccttttaat ttccaaatat
     7501 aatcttcaat cacatagatt gggatacgat atttaccatc gatcaaacca acttgatcaa
     7561 aagaaaaaat gatcccttcc tgcatcaaat tacgcatttc tttcaataat tccacactga
     7621 ttttttcaac aggtgtttct tttttgaaaa aagatttttt tacattgatc ttatctgaca
     7681 agatcgaact taatttacta gcaaaaatcc tataaaattc atctaaatag cgataataaa
     7741 tccgattcag ctgatcatta agatcgaagt atacaaaatt attctgtagt ttataaaaaa
     7801 acggcgaatg aagatgtgtt ttcatatgac caaaatataa taattctgaa atttctaaag
     7861 gtgtcaattc tttcagcatc gttaaatcag taaagtcgat ccacttcaat tcatcgaatc
     7921 cacgcctgtt acgagaagag gaaaagaaac gctgaatatt ttctgtacca cggattactt
     7981 tcagccctgt atgggattca tattctccag cagaagcaga tggatcaagc aagagcaagt
     8041 tttgaggaaa atgcacgata ctgtttgcaa agtcagtagc agtcagccct tttgttaaaa
     8101 cagcattact tgtggtatca atatgtacat aaactgactc agtcatcatg ttcctcctct
     8161 tatttcctct taatctcttt tattttacaa catttgcatt aaaaaagctt gtttctttta
     8221 ttttaaaatt ccattacaaa atcacgtttt cattgaaaac actctcattt tcaattaatt
     8281 tttgtgattt ttgtattttt ttatttaaag gaggttttat gaattgtttt cttatggaat
     8341 ctacaaagtc gaatacgaga ctcatgacct ttttatgacg tactggtata cttgaagaaa
     8401 aaggagagcg cgatggagat aaaaaaagtt tacttttcac agaaaatgtc gctgtattcg
     8461 cctatccagc aagcaaaaca accccttcca ttttttcaaa gtcatcaaga aagtagagac
     8521 gctttttggc gttctgcaaa aaagcaatgt gcttttaaag cagtatcgaa aaaatatacc
     8581 gtctatttta ctattgctaa aagtagagga gatgttattt ttaataatct gttgattgaa
     8641 ggtgaaactt ttgaatggaa aaaatttttt cggtatatgg aagcttgcgc tcgttttttt
     8701 attaaagaaa actgttgttt tttatttcaa tctcctctat cagaagaatg gttgcgtatt
     8761 tttcataaaa acggctatca aggaaccaca caattaaata aaaagctggt ctatcataca
     8821 gcacttgttt taggtggagg aggagcgcac ggtgcttatc agattggtgt gtggcaggcg
     8881 ctgaaggaac atgatatcaa ctttgagatt atcacaggta cctcggtagg tgcgttgaac
     8941 ggcgcattga ttttacaagg agatatgaaa aaagctgtga atttatggaa aaagctgtcg
     9001 acaagacaag tgcttgcgtt accagaaatg gctgcagttg aagaccttcg tgaacgcttc
     9061 tatcgagaaa gacgccaaat gacgaaaaca gcactaattg aaggcggggt ttcttctgct
     9121 ccattggaaa agatggtcaa agacaatttg gatttgtcct tactcaaaca taatcccaaa
     9181 atacgtttat atacggtgtc tactaaactg ccagaactag cggaagtagt gactgatatc
     9241 caacagacaa aaacagaaga aataccagat tggatactcg cctctgcttc attttatccc
     9301 gctatggctt atcgaaagat cggaaaaaat aaatatgcag acggtgggta tcgcaacaaa
     9361 atacctattg atattgcgat taacaaagga gcaacagagg catttgtcgt agatgtgcaa
     9421 gggccaggcc cagcgaaaaa aattcgtatg ccagacattt ttatccattg gaaatgtcaa
     9481 acattgtgga cactaggcag ctttctgctt tttgatagcc aaaggaacca attaaattta
     9541 caattaggct atctggaaat gaaaaaacga ttaggctatt actatgggaa ctggtataca
     9601 tttcattctg ttaaacctgc aagaacatac tggcgaggat ttttgtctta cttaacaaaa
     9661 gaaattcagt tagaattatc ttttttcaaa agcataaaat tttggcaaaa actgcgtaat
     9721 ttatataaga acagggtcgt accagagaca tgcggattgg cgatgctaga acttttagca
     9781 aaaaaacatt ttttactgcc caatgaaatc tatcaagtag acatgatgat tcaaatgatc
     9841 tgccaacaag atataaaaag tatgccggat gatttattgg cacaaattgg tcaattaagt
     9901 actgaagaat ggcgaaggta tcgaaaatac cagcaaaaaa tacaaaatga gcgcacgaaa
     9961 actgtttatt ttgattatct gctacaagga aaacgcaagg atcgtttgca gcatgaattg
    10021 cttaagcaac cagttgacac attactgatc ctatatttat attatctcaa ggaggaacaa
    10081 ccatggcaca agaattttca tatgaaatcg tagaagaaat cgctatattg tcagaaaaca
    10141 acaaggggtg gcgtaaagaa ctgaacctag tcagctggaa tggtcgcccg ccaaaattcg
    10201 atcttcgaga ttgggcgcca gaccatgaaa aaatggggaa aggcctgaca ttgacgaacg
    10261 aagagtttga acaactgcaa aaagcaatag aaaatatgta atgaggaagg accctgtcga
    10321 atgaaaagta tgactggttt tggcaaagcc atccgcgaaa caaaagcgta tcagttagat
    10381 attgaaataa aaagtgtcaa ccagcgtttt ctagatgttc aaatacgaag tcctaaaatc
    10441 ctgaactttg ttgaaaacga ttttcgtcag cttattaaac agcagttaag ccgcggtcga
    10501 atcgaagtat ttgtgaatct agcttatatt ggcaaaaacg aaaaacagat cgtcatcaac
    10561 tgggaattga ttgattcact tatcactcaa ttgacagacg gaattcaaca acgatacggt
    10621 agtcaaaatc aagtgtcaat tagtcggatg gcggaacgct tcgtgctgga cgaaaacttc
    10681 gtaacggtag aagaaaaacc ggaagagagc atggaagaat tggcacagtt agctctagat
    10741 acattgaaaa gcgcacttga ttctattgag gcgagtcgag aaaaagaagg tcaagcttta
    10801 gctttagtgt tgaaaaagaa tactgaagaa ttcaaagaag tactaaaaaa actcgatagt
    10861 tttgtagaaa tctatgaaaa ggaatttcaa ataaggtatc aaaaaaagct ggaggaattt
    10921 cttggagcga aagtcgatga gcaacggttg ttgacggagc tagccatctt gttggaacgc
    10981 ggagatattc acgaggaact ggatcgtctg gcgatccatg tacaaaaact agatgaactt
    11041 atcgaagcac aatgtcctgt aggtcgggaa ttagatttcc tgatccaaga aatgaatcgg
    11101 gaaatcaaca cgatcggttc caaatcaaat gcaatcgaaa ttaaaaatca agtcgtccag
    11161 tctaagacga tcttagaaaa aatcagagaa caagtacaaa atattgaatg acgaaagagg
    11221 tagagataat gaaatattat gacgtaacgt tccacgaatt aagtggaaaa actgtggtta
    11281 aacgggatat cccttctgaa aaaaacggct ttgacgtatg gaaagatgcc tgtgcagact
    11341 ataatgagaa cgaactgttt attttgatca acgacggtgc ttacgtgacg atgaatcgga
    11401 aatttattgt tcgaattgac acagaagagg tagaagatcc gacagaaaaa gcacgttctc
    11461 gaaaagatga gatcatgggt gtagtgaata ctttgtccaa tatgggcttt tagcaaggat
    11521 gctgaacaaa gaaatacaat aaataaacag aattggattt cttcgcttag cttctttgct
    11581 ctaggaatac atttaaaggt tgtgacaaaa ttcgtgtcac aatctttttt tattttgaat
    11641 aagtgatgtt caaaaaccat tttctcggta ttaagccaaa tttatgcaaa aatgtgagaa
    11701 actattttca caaaattttt cttcatactc gaaactaatc ggcttcttca cgacctctta
    11761 tacacggcgt tcaaaagctt gcatctgcga taataacccc aaccaaacga aaaacatgag
    11821 aagctgttta tgtttggctg ttcttattac ttgatgcagg acagcttttt tacgacctct
    11881 tggtacaggg tagctttttt accaatttct agctataatt aaagataacc ttgaaagttg
    11941 cagaaaaaaa gtgcaaaata aacgataagt acaaaaagac tagttgaaag gacgtcgtca
    12001 tgtcagagcg tggattatta atcgtgttgt ctggtccatc cggtgtagga aaaggcacag
    12061 tacgaaaagc aatttttgag agtgaggata atgattttca gtattcgatc tcaatgacta
    12121 cccgtaaaat gcgtgaaggg gaggtcgaag gcgttgatta tttcttccga agcaaagaag
    12181 agtttgaatc aatgatcgaa gctggagaga tgctggaata cgcagaatat gtcgggaatt
    12241 attatggaac tcctttgtca tatgtcaaca agacattgga tgaaggaaaa gatgttttct
    12301 tggagattga agtccaagga gcaaaacaag tcaaagaaaa agtgcccgat ggtgtattca
    12361 tctttttgac tccgccagat ttagcggaat taaaatcacg gatcgtaggc cgaggaacag
    12421 atgcagatga agtaatcgaa aaacgaatga aagttgcaaa agaagaaatc gaaatgatgg
    12481 ctctttacga ctatgctgtt gtaaatgatg aagtgccgct agcagttgaa cgaatcaaaa
    12541 acattattgc cagtgaacat tttcgagtag atcgcgtaat cggtaaatat attaaaatgt
    12601 taaaggagat gtagtcttat gatgttaaaa ccttcaattg actcactatt agatcgtgta
    12661 aattcaaaat attctctagt gattttagca agtaaacgcg cgcatgaatt ggatgcaagt
    12721 gcacagccta ctctagattc ttttgattca gtaaaaagcg tcggtcaagc tttagaagaa
    12781 atcgatgcag gaaatgtagt caatgatcca catcctgaat taaaacgtga acgtttgaag
    12841 atggaagaag aggaacgtca agcacaaaaa gagcgtgaac aacacgaatt ggaatcacgt
    12901 atccgtgaag agcaaaaaat gtaataaaga aaaatagagc gaacatctaa aaagaaaagc
    12961 aaacccagct gtttcctttt caaacattcg ccacttattg tctggtagaa aaggctgtgt
    13021 ctgaactttt tgacacagtt cttttttttc ttatctgctg ttaatttttt gttaggattt
    13081 ggcagaagat aagacaaatc catcgatctc tagtaaaata ggcaaggaac tttttgtcgc
    13141 ttgataagaa aagacaaaaa aaggaggaaa cgaaccatga cattaacagc tgaagtcatt
    13201 gtggacgtac ctactatgca gaccgatcag ccattcactt atttggttcc ctctgaaatg
    13261 gaaacagctt tgcaagtcgg gatgcgagta gaagtccctt ttggtaatgg caatcgtcat
    13321 gtccaaggat ttgttatggc tatccaaaaa tcagaagaat cagctaatcc atctttgaag
    13381 gcagttattc gtttgctaga ccttgctccg gtggtcaatg aagaattact ttcattagct
    13441 gattatatga aaaaaattac ttacgcattc aaaatcactt gtctacaaac gatgcttccg
    13501 agtgtgatga aagcagaata tgataaattg atctatcctt tggcagacac accagaagtt
    13561 caggtattgt ttgatcagcg agaagtgatt tcctggaagg aagcagaaga agaaggcagc
    13621 ctttctcaat tgattcgctg gcgccaagag caattagtag atatcaaata tgaagtccat
    13681 acacgaaata aagtaaaaac gattcgtttg gtccgttctt tgttaactga aaagcaaatc
    13741 gaagaagaat gggcaaaatt acggcaaaat gccaagaaac aaaaagaact tcttttgtgt
    13801 ttatctgaac tgagccaaga agaaccaatt gcttatttca aaaataaagg aatcagtact
    13861 gctgtcttga atcaaggaaa agaaaagggc tggctggagt ttatagaatc agaacgttat
    13921 cgcgaccctt ataaagaccg agtatttgac caaacgacgg cattggaact gaatgcagaa
    13981 caaaaaaatg ctgtcgaaca aatcatcaca gcaggtcagc agcaaaaaga tcaggtcttt
    14041 ttgctggagg gaatcactgg cagcgggaaa acagaagtat atcttcaggc aatcgctgat
    14101 gttttgtcag aaaataaaac ggctattatg ttagtgcctg aaatcgcatt gactccgcaa
    14161 atggtcgagc gattcaaagg acgcttcgga gaatcagttg cggtcttaca tagcggactt
    14221 tcccaaggtg aaaaatatga tgaatggcgc aagattgagc gggaagaagc gcaagtagta
    14281 gtaggcgcac gatctgctat ttttgccccg ctgaaaaata tcgggctgat cattatcgat
    14341 gaagaacatg aatcaagtta caaacaagat gagacaccgc gttatcatgc gagagatcta
    14401 gcaatttggc gcagtaaata tcatcattgc ccaatagttc taggaagcgc gacgccctct
    14461 ttagaatcaa gagcacgagc tcaaaaaggc gtttatcagt tattgcagtt aaaccaccga
    14521 gccaaagcag ctgcacagct tccagctatt gagatcgtag atatgcgtga agagtttcag
    14581 aatcatcgga cctctacttt ttctgctaat ttgcaggaaa aaatacaaaa tcgattagac
    14641 aaaaaagaac aaactgtcct actgctcaat cgtcgaggat attcgtcttt tgtgatgtgt
    14701 agggactgtg gatttgttct tccatgtcct aattgcgata tctctcttac actgcatatg
    14761 gatacacgtt caatgcgctg ccattattgc ggacatgaag aacctattcc caatcgctgt
    14821 cctaattgtg gcggcaataa aatccgctac tatggaacag gtactcaaaa agtagaagag
    14881 gaactccgag aactttatcc gcaagctagg attttgcgta tggatgtaga taccactcga
    14941 agaaaagggg cacacgaaca gattttacaa aaattcggtg caaaagaagc agatatatta
    15001 ttagggacac aaatgattgc taaaggtttg gactttccag aagtgacatt agttggggtg
    15061 ttgaatgctg atacctccct gaatttaccg gattttcgtt ctagtgagca tacattccaa
    15121 ttgcttaccc aagtttcagg acgagcaggg cgagcggaaa aagcaggcga agtcgtgatc
    15181 caaacattca atcctcaaca ttacgccata gaattggcta aaaaacaaaa ttatgagcag
    15241 ttttatcagc aggaaatgca tgtacggcat cgtggaggct atcctcctta ttactttact
    15301 gtgaaaataa ccgccagcca tcctgaagaa caagtagcgg caaagaaaat ctttcagatt
    15361 gccaatcagc taaaagaagt gttaagccct caaagcttgc tattagggcc aacgcccagc
    15421 atgattttga gagtgaaaaa ccgatattat tatcaactaa tcatcaaata taaacatgaa
    15481 ccgaatcttc ctcatgtttt ggatgaaatt ttgaatggaa gccagaaaga acagcgacaa
    15541 ggtctttttg tggcaattga caacgaacca ttaaacttta tttaagaaag ggccaaatta
    15601 tgcgctatcc tatattgatc caccctaatg ataaattaaa aagaactgct cagccaatcg
    15661 atgtaatcac ggacgagaca atcgctttat tagacaattt atatgagaca atgattgcga
    15721 atgacggaat agggatcgct gctcctcaag taggacaaaa caagagaatt gctgtgatcg
    15781 aagtagatga aggcgagaaa tttgaactga ttaacccaga aatcatcgaa gcaaaaggtg
    15841 aaagccttga cgtagaaggt tgtctcagta ttccgcatgt ctatggaacg gtcaagcgag
    15901 cagatgaagt gaccgtgcgt tattatgatc gagacggaga agagatagaa gtgacggctt
    15961 ttggctattt agcccgtgcc ttccagcatg aaatcgatca cttagacggt atcttgttca
    16021 ttgagaaaat gatacagcag atcccggaag aagaacttga agaatatatg gaggaacatc
    16081 ttgatgacta agctagtatt tatgggcaca cctgcttttt ctgtgccgat tcttgaaggg
    16141 ctgatagaat caggttacga gatccaggca gtggtaacac agcccgaccg tccagttggg
    16201 cggaaacgag taattacgcc gacaccggta aaaaaagcgg ctctgaagca cggtatccgt
    16261 gtgctgcagc cagagaaaat ctccggttcg ccagaaatgg aagaaatcat tgaattggtg
    16321 cctgatgtga tcgtcactgc ggcatttggc caattcttgc ctgaaaagtt attgcaagta
    16381 ccaaaattag gggcaatcaa tgtccatgct tctttacttc ctaaatatcg gggaggtgcg
    16441 cctgttcatt atgccatcat gaatggggag aaagaaactg gtgtaacgat catggaaatg
    16501 atcaaaaaaa tggatgcagg cggaatctat gcgcaagaaa gcataccaat cactaaacaa
    16561 gatgatgtag ggacgatgtt tgaaaagctt agccttttag gaagaaagct attgctagaa
    16621 acattgccca atattttgga tggacaaaaa ccagtacctc aagacgagtc agaagcaact
    16681 ttttcgccaa acatcacacg ggaacaagaa gcaatcgatt ggcataaaac tgcagaagaa
    16741 attgacaacc aagtacgcgg aatgcgtcct tggccaattg cattcacgac ctatgagggc
    16801 atacgctgga aattattatc ggttgaagta ttggcagaaa ctacagacaa agcacctggt
    16861 acgatcatcc ggaaagacaa aaaaaatcta tggattgctt gcggtaaaca gaccgtctta
    16921 gccatcaatg aattacagcc agctggtaaa ggaaaacagg ccgtacatga tttccttaac
    16981 ggaagcggtc agcaagttca gattggtcag caggtgaaat aatgaaaaaa atccctaaaa
    17041 aaatgaaaaa atcagtacga tatatggcat tactgacatt agagcgagtg aacaagggtg
    17101 gcgcctactc gaatcttttg ttgaatgaga tgatcaaaca aagcgagcta aatcaaaaag
    17161 atgtgggttt atttacagaa ttgatctatg gtacgattag ccatcagcga ctattggaat
    17221 tttacgttgc accattgatc gctaaagcaa aaaaagtgga tgactgggtc aagatgcttc
    17281 tttatctttc tgtataccaa ttagaatttt tagataaagt ccctgcacat gctattttaa
    17341 acgaggcggt cgaaatcgca aaagtaaaag gaaatcctgg gacagggaaa tttgtaaatg
    17401 gtgtcttgag gaattatcag cgtcaaggag caccagatct gcaaggcatt gctgatccta
    17461 tcgaaagact ttctgtcaaa atcagcctgc ctctatggat gacgaaacga ctggtggatc
    17521 aaatcggata cgaagaaact gaaaagctcg gtttatcgct atatcagcct agccatgcta
    17581 gtgcccgagt agatacacgc cgactttcca gagatcaggc catcactgtt ttgcaagaag
    17641 aagagatcac agctgaaaaa agccaagtca gtccatacgg tatagtagcg aaaaaagggc
    17701 atttagcttc tagctcattg ttccacgatg gcgtcatgac gatccaagat gaaagttcga
    17761 tgcttgtagc gccgtctatg caaatcgagc ctgaacatca tgtcttagat gcctgtgcag
    17821 cacccggagg taagacgacg catattgctt cttttttaga tccgtcatgc ggtggagaag
    17881 tggtcgcttt agatgtacac gaacacaaag tcaaactcat tgaagataat gcagcacgtc
    17941 taggtgtctc ttctgctgtc tacgctcaaa agatggatgc tagagaagtc agtaaacagt
    18001 tcgaagcaga gcagtttgat cggattttag tagacgcgcc ttgttcaggt cttgggctgc
    18061 ttcggcgaaa accagatatt cgttataaaa aacagccgga tgaattagcg aatttaccaa
    18121 aaatccaatt ggaaatttta gaaagctgcg caccaacttt aaagtcttcc ggtattctga
    18181 cttatagcac ttgtacgatc ttaaaagaag aaaaccaaga cgttgtttct gctttcttgg
    18241 aaagacaccc tgattttgag aggattgatg ttttgaccaa tcctatgcta caatcatcaa
    18301 tcgaagagaa aatgttgact ttgtatccgc atcagtttta cacagatgga tttttcattt
    18361 gttgtttgcg caaaaagtaa tgaggtgaaa aaatgcagat tgaatatcaa tcagatgtag
    18421 gaagacgacg taacacaaat caagactatg ccagtgtatt taccagccgt tcaggtctca
    18481 agcttgctgt attagcagac gggatgggtg gtcatcgtgc cggagacatt gccagtcaga
    18541 tggcggtaac gaatctaggc gaagcttggg aaaaacaaga agctgttgac gatgaaaaaa
    18601 tcgcccaatg gttcattcaa tcgattcaag aagaaaatat gctgatttat cagcgtggac
    18661 aagaacagcc ggaatataac ggtatgggaa caacaatcgt agcggccgct ctgtcagatg
    18721 actggttcac gattgccaat gttggggata gcagagctta tttgattcgt gaagataaaa
    18781 tcatgcaatt gacggaggat cattctttag tcaatgaact agtcaaatca ggagaaatca
    18841 gcgaagaaat ggcagtgaac catccaagga aaaatatttt aacacgttct gtcggcatgc
    18901 ctggaacagt cgaagtagat gtttctactc atctttggca actaaaagat cgactgctgt
    18961 tatgttcaga tggcttgacg aacatgctgt ctgaagaaga aataggaacg atcatcaatc
    19021 aagaaggccc attgtctcat aaagtaactg agctgattga tcgtgccaat gaagcaggcg
    19081 gagcggataa tatcaccgtt cttcttatcg aatataagga ggacagtgta tgatcgagtt
    19141 gggcaaaaaa ttaaatggcc gttatcaaat cactggaaat atcggtagcg gtggtatggc
    19201 aaatgtattt ttggcgcacg atttaatctt ggatcgtgat gtagcagtta aagtcttacg
    19261 atttgacttt caaaatgacc agactgcgat tcgacgattt cagagagaag ctttagcagc
    19321 aacagaactc gttcatccaa acattgttag tgtttacgat gttggtgaag aagacaacat
    19381 gcagtatctg gtgatggaat acgtcaaagg aatggacttg aaacgatata tccaaacaca
    19441 ttatcctgtt ccttatgaaa cagcagtaaa tattatgcag cagatccttt ctgctatttc
    19501 tttagctcat aaccatcaaa tcatccaccg agatttgaaa ccccaaaacg tactgatcga
    19561 taatgaaggt gttgtaaaaa tcacggattt cgggattgct atcgctttat ctgaaacatc
    19621 gatcacgcag acgaatacga tgctaggatc tgttcattat ctgtcccctg agcaggccag
    19681 aggaagtatg gcaacgaagc agtcagatat ttatgcttta gggatcatcc tttatgagat
    19741 gttgacgggt agcgtaccgt ttgatggtga atctgctgta acaattgctt taaaacattt
    19801 tcaagatgat cttccatcta taaaagcact tgatccaaat gtgccacaag cactggaaaa
    19861 cgtgatttta cgggcaacgg ctaaagaacc tgcagaccgt tataagtcag ccgaggaaat
    19921 gtctgatgat ctttctacag tattatcacc agctcgtgca aacgaagaaa aatggcagcc
    19981 tcatgtgatg gataatgaaa caaaagttat cacacccctt acggaagatg caccaatgcc
    20041 tgattcattc aaatctatgc ctctgccaaa agacaagcca gaaaacgaaa ccttaccaga
    20101 ggaggaagaa gttctgcctg atcctaaaaa gaaaggacag aaaaaatggt ggctgatcct
    20161 attagctatt cttgctgttt taggaatcgg aacagctgta ttctttgctt ctggcgggcg
    20221 aggagaagtt gttgttcctg atgtttctga tctttctgaa gcttctgcta gagaaacact
    20281 cgcaaaagcg aaattagaag tagcaacaaa aacagaagaa attgcagatg acaaaatcga
    20341 agaaggtaat gtagtgaaga cagacccggc agcgggaacg acagtcaagc aaaaacgcga
    20401 agtcactctg tatattagta gcggcacgaa aaaaatcaaa ttagatgatt acagtggtaa
    20461 aagttatgaa gatgccgaag caagtttgaa agaattaggg ttttctgctg acttgattca
    20521 aaaagaagag gaattcagtt cagaggtaga aagcggaatg atcatttcgc aatctccatc
    20581 agaagataca gaagtcgatc ctaaaacaga cacgattact ttcacagtca gcaaaggacc
    20641 agaaacagtg actttgagta gtcatgtagg agaaaactac gaaactgttc gtaattggct
    20701 tgttacacaa ggattcaata tcaataatat tagtgctact tatgactata gcgacactgt
    20761 accatcaggt tcgatcatca gtcaggatcc agcctcgggt gaagttgtag cagaagaaac
    20821 ttacatcaat tttgtcgtaa gccaaggaac agagcctaaa ttgaatgata tctccggcta
    20881 tacaaaaagc gaagcacaaa gttatttagc tagtgtcggt gcagaatata tcgggcatga
    20941 gacttccgaa tattcaaaca cggtggataa agataaagtg attcgtacag taccaggggc
    21001 aggaacgaca attaccaaag gaatggttgt gaacgtaatt tactctaaag gaccagatcc
    21061 agcgtcttct agttctgaaa aagactcttc agacagttct agcagctcaa gtagctccag
    21121 tagcacaagc aactcttcaa aagaagaatc ttccagctcc acaagttcat cagctagcac
    21181 ctcagaaagt tcaaaataag tgctgaataa aatatatcag aagggaatgc gacattgatt
    21241 tgttgcattc tcttttttgt gtttctatct cttttgatca attttcaagc catatacttt
    21301 gagaagtttg catggaataa cacctacttt cctcttcgta tggtaaaata aacaaaaacg
    21361 gaggtggcac ttatcaaagg acaaatcaga aaagcaatca gcggttttta ctatgtatac
    21421 gcaaacggag agacctatca aacaagagga agaggaaatt tccggaatcg gaaaatcacg
    21481 ccgcttgtcg gagatcaggt gatctttgag agtgaaaatc aaacagacgg ctatcttctg
    21541 gaaatcttgc cgagaaaaaa tcaattagtt cgcccaccag tcacaaatgt tgatcttgga
    21601 atcattgtca caagtatcgt agagcctgca ttttcttata atctacttga ccgctttcta
    21661 gttaccttgg aatacgagtc gatcgaaccg attatttttt tgacaaagac cgacttgtca
    21721 gacgacccag aaaaaatcga agagatcaag caaacctata ctaaaatagg ctatccggtg
    21781 attgtaccca atcatacggg tgatacgaca gaactgatcc gttacttccc agaacggcta
    21841 tcggtcttta tgggtcagtc tggagcagga aaatcgacgc tactgaatca aatatcaccg
    21901 gaactgaatt tagcaacagg agagatttct gatgcacttg gaagaggacg gcatacaaca
    21961 cgtcatgtgg aactgattcc tatctatggc gggttagtag cagatacacc aggatttagc
    22021 tcgattgact ttttgacaat tgaagcagga gaactgccga gacaatttcc tgaatttgta
    22081 gaagcttctg tccattgcaa atttagagag tgcatgcatg ctaaagaacc tggctgcgaa
    22141 gtaaaacata gagtagaaac aggtggtatc gcacagacac gttacgacaa ttatttgcaa
    22201 ttcttacaag aaatcgaaaa aagaaaacca atgtatgcaa aaaaagaagg taaacgaaaa
    22261 taggaggaaa caagaatgaa gatagcacca tccattttga gtgcagattt tgccaattta
    22321 caaagagaca ttgagttagt ggaaaaagga ggcgcagatt acatccacgt agatgtaatg
    22381 gatggccgat ttgtcccaaa tatcacatta gggccaaata ttgttcaggc aatccgtcct
    22441 gttactaaat taccgttaga cgtccatctg atgatcgttg atccagaaaa ctacattcca
    22501 gcatttgcaa aagctggagc agatatcatt actgtccacg tcgaagcaac tcctcatatt
    22561 catcgggcac ttcaaatgac aaaaagctta ggagttaaag ctggagtagt tatcaatcca
    22621 ggaacaccag tctccatgat caaacatgtt ttgcctttgg ctgatcaagt actagtcatg
    22681 actgtcaatc ctgggttcgg cggacaaagc tttattgaag aaacagtaga aaaaattacg
    22741 gaactcgctg atctacgtag agagaacaat tggacttatg caatcgaagt agatggcgga
    22801 atcgttccag aaacagcaaa aatctgtaaa gatgcagggg cggatgtctt cgttgcagga
    22861 tcatatatct atggagccga aaatccagtc ggacaaatcg aacgattaaa agaggcatta
    22921 gactaaatga aaattttatt agctgctgga ggtaatccgc aaaactggcc gcagtctctg
    22981 ctgtccaaat acgatgccta tgtcgggata gaccgtggtg cgctgtacct gcttcaagca
    23041 ggctacccag tagatctggc agtgggtgat tttgattcat tgtcgaacga agagcagaag
    23101 gaagtatttg caaaagccaa agagtatacc cgttcccctg cagaaaaaga cgatacagat
    23161 acacagttag gtatcttaaa aacatttgaa cgttacccgc aagcggaagt cactttgatt
    23221 ggagctacgg gcggaagatt ggatcatctt ttagccaatc tctggttagg tatggaacct
    23281 cgcttcaaac cctttgccca tcagcttatt atcagagatt gtcaaaatta tttgtcttat
    23341 tatttgcctg gagagcatat agtaagacag ataccagata tgaagtattt agcttattgc
    23401 tgcttgactc ccgtctcgaa tcttacatta aaaggcagca agtatgtatt ggaaaataaa
    23461 gaagtagaat atccgacatc ttatgcaagt aacgaatttc tttcagatac cgcagaattt
    23521 tctttttcag atggtatgat tgcagttatt caaagcaaag attgatccat aaacaaataa
    23581 aaaaatttgt ctttcaggct aaaaatgaca gaatctatta tttgctaaaa aaaagcttaa
    23641 gaggagcaac ttaccttttg tttcacggca tttagtgata aaattgaaca caagagtcta
    23701 attaattcga ggtgtctttg tgaagaagtt caatcctaat aaaaatatta ttattacctt
    23761 ggttattgtt atcatcgtgg tgacgatact aagtgtgacc gctgcgcagc gagcgaatga
    23821 aggaaagccc aatttcttcc aatccatcgt taatgacagt gttgcaatgg ttgataaaac
    23881 aatctctgcc ccagtacggt gggtacgaaa tggagtagat tctgtccata atcttttttc
    23941 aacttatgcc gaaaatgagc ggctaaaaga aaaaatcgat agctatgatg acgtttcaca
    24001 gcaaaacaag aattacgaga gagaaatcga agcgttgaaa aaagaacttg agttgaatca
    24061 gacgctgact agttacgaaa aagtaacggc aaatgtcatt actcgttcgc ctgacagttg
    24121 gcaggacatg ctgatcgtag acaagggttc taaagacggt atcgaagcaa atatggcagt
    24181 catgtcgcaa aaaggattga ttggccgagt gatcgaggtc aatacggctt cgtctaaaat
    24241 cgaattactg tcatcctcta atgaaagctc caatcatttt ccagtacggg tatcttcggc
    24301 taatggcgaa gcgtttggtt tgcttaaaaa ctatgatgaa aagctccatg ccttagtggt
    24361 gacccaatta actggtgata cggatatcaa agaaggggat gttgtccaga catccggtct
    24421 tggagggaat tctccagcta acttgccgat cggtacggtt attaaaacga aaccagatag
    24481 ttatgggctg gatcgggaag tttatgtgaa accttatgca gaaatgtatg acgtgtcagt
    24541 tgtgacgatt gtccaacggc tagcggagga ttagtccatg ttgaagaaag aaacaatgaa
    24601 gtactatctg ccaatcgttt tgttcttttt gatgttgata gatggtcatt taacaagaat
    24661 gctaggggag tggtcgaaag gcacctatat gtcaaatgcc cactttctga tattggcatt
    24721 attatgttgc agtatggcgt ttgaaaaacg ttatttactg attaccacga ttgttctcgg
    24781 ggctatctat gacgcttact atattggcgt tatcggtatc tatgcagtag ctctcccttt
    24841 aattgtatgg ttgatgtatg taatgaaaga cgttatccat gtcaacatct ttactgaatt
    24901 tttcagtatg atcatctttg tcacgggtta tgaattgttt acgatggtgg tccagttgat
    24961 ttttaaatta gcagtagtaa ataacacgta ttttattaca aggtttttag gacctacact
    25021 gctgttgaac atgattatat ttgtattatt catttttccc tttaagaaat tattcagtga
    25081 tgaataattt ctttttttat ttttaggtcg aaagcgaaaa agaatcgttg ttatcttaag
    25141 aaatgctaga cttaacgcgg ctttttcatg atcattttct gaaacgaaca gttttgtcat
    25201 ttttaaaagt tgttttgaaa catttttgta acataactat tatatcgttg acacactgct
    25261 atggtacaat ttttatatcg ttaagaaaga cttaaatata tttagatatt tattaaatta
    25321 agccagtcgg aggaatgaag agtgaaaaag agtttaatat cagcagtaat ggtatgttca
    25381 atgaccttga ctgccgtagc atcaccgatc gctgccgcgg cggacgattt tgattctcag
    25441 atacaacagc aagatcaaaa gattgcagac ttgaaaaatc aacaagcaga tgcacaatca
    25501 caaatcgatg ccttagaaag ccaagtttct gaaatcaaca cacaagcaca agacctatta
    25561 gcaaaacaag atacattgcg tcaagaatct gcacaattag taaaagacat tgcagattta
    25621 caagaacgta ttgaaaaacg tgaagataca atccaaaaac aagcacgcga agcacaagta
    25681 agcaacacaa gctcaaacta catcgatgct gtattgaatg ctgattcatt agcagatgct
    25741 attggacgcg ttcaagcaat gacaacgatg gtaaaagcaa acaacgactt gatggaacaa
    25801 caaaaacaag ataaaaaagc tgtagaagac aaaaaagctg aaaacgatgc taaactaaaa
    25861 gaattagctg aaaaccaagc tgctttggaa tctcaaaaag gcgatctttt atctaaacaa
    25921 gctgatttga acgtactgaa aacttcatta gctgctgaac aagcaactgc tgaagacaag
    25981 aaagctgact tgaaccgtca aaaagcagaa gctgaagcag agcaagctcg tattcgtgaa
    26041 caacaacgtc tagcagaaca agctcgtcaa caagctgcac aagaaaaagc tgaaaaagaa
    26101 gcacgcgaac aagcagaggc tgaagcacaa gctactcaag cttcatctac tgcacaaagc
    26161 tcagcaacag aagaatcatc agctacacaa tcttcaatga ctgaagaatc atcatcagct
    26221 acacaatctt cagcaacaga agaatcaaca acacctgaat cttcaacaga agaatcaaca
    26281 gcgcctgaat ct
//
LOCUS       DACEEZ010000030        25849 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20313_2017
            SAMN12639966-rid9879223.denovo.030, whole genome shotgun sequence.
ACCESSION   DACEEZ010000030 DACEEZ010000000
VERSION     DACEEZ010000030.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639966
            Sequence Read Archive: SRR10023158
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 25849)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25849)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741683.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741683.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:44:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,016
            CDSs (total)                      :: 2,962
            Genes (coding)                    :: 2,889
            CDSs (with protein)               :: 2,889
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 36 of 73
            Pseudo Genes (incomplete)         :: 29 of 73
            Pseudo Genes (internal stop)      :: 18 of 73
            Pseudo Genes (multiple problems)  :: 8 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25849
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639966-rid9879223.denovo.030"
                     /isolate="E20313_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            369..557
                     /locus_tag="IWO89_001320"
     CDS             369..557
                     /locus_tag="IWO89_001320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0456087.1"
                     /translation="MELIVFTNNGQTYHFYEVENFKPTTTGFSFIYTGKATGVTRSAT
                     FNNTSTAGYALAEVQNES"
     gene            547..807
                     /locus_tag="IWO89_001321"
     CDS             547..807
                     /locus_tag="IWO89_001321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0456088.1"
                     /translation="MNHEKFIEKCKAIVRERIENEIADLSGAVPEFSIFIVWSCKTLQ
                     NSKALVSASLKGAPYFEITLNGDKGEIYVDTYLKKSNECIKV"
     gene            848..1075
                     /locus_tag="IWO89_001322"
     CDS             848..1075
                     /locus_tag="IWO89_001322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002370649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0456089.1"
                     /translation="MRNYWYVSLTNEYPRTIGDCSVRVVRSVQIKGKYSIVEMLREAT
                     PNEVDKCKLIYCGHGYWKDEYIQYNIERYIK"
     gene            1140..1712
                     /locus_tag="IWO89_001323"
     CDS             1140..1712
                     /locus_tag="IWO89_001323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0456090.1"
                     /translation="MKLKDYIREGYNVVTAPNLAYKIQEDYPNALVFTDRALDAIPIG
                     KLLVDRFYSNNPAVLSAKPLQNAYAIERFTCEFTGYETVVPMTGETGRKVIQQIKKRI
                     AELTLDDGSNTNLLEIKLRDTDSVPEVYYKGQRMDEMPIGLVDITYHWDTEEYECQVP
                     KGANDITIEYYDSHNNKYLDRKIIGHKRDM"
     gene            1713..2024
                     /locus_tag="IWO89_001324"
     CDS             1713..2024
                     /locus_tag="IWO89_001324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002358084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HAQ0456091.1"
                     /translation="MIDVSSKQARARFYGSTEWRKLRSQCLERDHYECQWCKAEGRLT
                     TQHDSILEVDHIKELEYHPDLALDINNLRTLCKDCHNKRHNRMKYRGQQKKRKWDDEW
                     W"
     gene            2015..2275
                     /locus_tag="IWO89_001325"
     CDS             2015..2275
                     /locus_tag="IWO89_001325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0456092.1"
                     /translation="MVVRKTANRDDVKRAEAIAMVDKAIDKLVLFKSQLIEGTTTVED
                     ISINNRLIGNANEVTGTFNINVDIDYTLSPFAHIVESNEQDG"
     gene            2436..2795
                     /locus_tag="IWO89_001326"
     CDS             2436..2795
                     /locus_tag="IWO89_001326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0456093.1"
                     /translation="MADLQERNRLVSAEERRLNKLFKDIDKDKKKVVSGLITQAARLK
                     ILLDEMWIDISEKGDYELFSQSENQKPYERERPVAKQYNSRDQAYQRVIKQLTDYLPE
                     EKREPTKNAALDGSDLL"
     gene            2792..4435
                     /locus_tag="IWO89_001327"
     CDS             2792..4435
                     /locus_tag="IWO89_001327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase large subunit"
                     /protein_id="HAQ0456094.1"
                     /translation="MTTLKPFYFDEYVDLYEKGVIPFNKERIQLITYLKNEVLVRDDI
                     YFDEEMIQKFIKYTEKNFFPLAKYQKFITPFIFCYQKEADEVFFDEILNSIARGGGKN
                     GFMSARDSFFISPLYGVRNYDVTITANSEKQGKVSFKEVYEMVQMRKLEKQFYLTKMA
                     IVNNVTNSIFSYRTNNPKTMDSARDGCLEFDEIHQFESSDLVDIQRSGLGKIKHPRTF
                     YNGTNGHVREGFYDKMLERAQKIFSGENKNDRLFPFICKLDNIDEMDNPKMWSKANPM
                     FEEDSAYSKRLFSTVMKEYKKLEDEPSGRREFVVKRMNFTEGDAESDITTHEKLMATK
                     QELPNLNGRSCVAGFDYASIRDFATVGLLFKIDDKFIWQQHSFARKKFLDTFKLKAPI
                     KDWEKKGLVTIVDEPSIDPRHLIDWLNEKRKIHQIEIVCADGFRMDLLKPLLEKESYN
                     FEFIRNIRGVQSKVAPIIEDGFANERFIFGDDPMMRWYTNNSFIKEDKLGNKTFLKKE
                     PVRRKTDGFHAFLAALYKREEITDMDYEEAFDMLDEIEF"
     gene            4470..5552
                     /locus_tag="IWO89_001328"
     CDS             4470..5552
                     /locus_tag="IWO89_001328"
                     /inference="COORDINATES: protein motif:HMM:NF028182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0456095.1"
                     /translation="MKKRYFLFVTIFIIIAICVLTVFLPQITEYLNYRIFTTKVDDSV
                     TSLSRLEFIKICASVFGPILTVMVFVNTLNMQKKTEQKQNELEVKQAKAENERAKKEL
                     LTQIDREFYFLLNMFIEIQKDQNAKQAVIALQKLAIFNEKGFNQDYAFTSKDLSDSEI
                     GSTIEQSGPLKGTVEYIKFETFDVSKSMGNYQTKTKDVATSLHDQKVAILNEQYEKMQ
                     IYLGRYFKMFHRIVKTLNEYYDDYNDFDVKRYTKYIGTLRTQISPAEFQVILFNSLYI
                     KRGFGLGIQLIGSGFFGDDFDFETNQHFETSINEQWFLSLSTVDSDNSIKRQKLSEYI
                     KELGSVEYKKIANFESLYKLFNETKL"
     gene            5596..6768
                     /locus_tag="IWO89_001329"
     CDS             5596..6768
                     /locus_tag="IWO89_001329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HAQ0456096.1"
                     /translation="MSLFDIFKQSIRNEEPSEWIPDFVYGEEVSARAYLKIMAKNTVL
                     DFVARTMSTLEVKFKNKDGTSDWDYILNVRPNSDMSAATFWQKFFYRLLDDNEVLVIF
                     TDDNQLLIADDFSRTEYAVYDDVFTNVMVKNFVFERSWNMSDVIYMEYNNEELERFTR
                     GLFEDYAALFGRILEVAMRNNQIRGSVSIDSTGTANDEKGKDGKTRSQRLQEYIDRIW
                     NSFKTNSVAIVPKVKGFEYEEYTNKQGVSNQSLDELNKMKSSLIDDVANAIGVPTALI
                     YGEKAELDSNIKAFRKLCIAPLVKKLQDELVAKIISRQEYKNGERIEVKKVLQKDLLE
                     YATQIDKITSGGAFYIDEVRDEVDYDELPNNEGKIRIRTKNYEETVKGGENDNAES"
     gene            6755..7459
                     /locus_tag="IWO89_001330"
     CDS             6755..7459
                     /locus_tag="IWO89_001330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010749273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Clp protease ClpP"
                     /protein_id="HAQ0456097.1"
                     /translation="MPKAKKVPFQFTNEIQNGKHILTLSGNVQKKYWRDDDVINAKDI
                     RDSLDTVTDDIVIKLNSPGGDVFEGIEIYNYLKDHPSNITVEVTGLAASAATFIVAGA
                     NDVIMNVGTSLMIHEASTFAWGNKQDIQKTLNALETIDDSILAIYSDKTGQSVDKLRE
                     WMNEEKWFTADEAVKYGFANSVKREEPQNEPQNIAAMIQDAVAVAIANLKEPVTNKIE
                     QESKQKSLIARLRKGE"
     gene            7464..8624
                     /locus_tag="IWO89_001331"
     CDS             7464..8624
                     /locus_tag="IWO89_001331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein"
                     /protein_id="HAQ0456098.1"
                     /translation="MLKITDKTADAKKAFNAISSKEDATPEQINAALEEYVTAIAEDA
                     GKQVRAEYEELKNVTDNRVLEARGIPTLTAEETKFYNEAVKSGGFDSDVVWPETILER
                     VFENLQNDHPILGLINFTPTVGRVKVIRARRKGVAVFGPLHKDLEGQLDAEFGATEFV
                     QLALTAFFLISNDTLDLGPRWIDRFINLSLSEAVRDVWAKKIITGSGKDEPIGLLKDL
                     DGAVTSGVYPDKASAGTLTFAKEKIVLELAGVMKKLSKYTYKVDGDDEGEVKYRSVAG
                     RVYLIVNPVDYWDIVARVTFANLNNVYGTTMPFIAVDHIIESVDVPESKCIAYVDKEY
                     EATQSRAEKVYVYKETFAMKRATLYAVDMLGNGYPTNNDAAQVYDLDFTAGE"
     gene            8700..8978
                     /locus_tag="IWO89_001332"
     CDS             8700..8978
                     /locus_tag="IWO89_001332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage gp6-like head-tail connector protein"
                     /protein_id="HAQ0456099.1"
                     /translation="MNTEEFIKEYKARFRIFHTSEDEDIGKQLESGFADIKSLIGSFD
                     PSTYEKGKELVYERTRYLRNESLEYFYDNFQTMIMDASIDLAGAGYAN"
     gene            8959..9330
                     /locus_tag="IWO89_001333"
     CDS             8959..9330
                     /locus_tag="IWO89_001333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002380685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage head-tail adapter protein"
                     /protein_id="HAQ0456100.1"
                     /translation="MLDMPIKSNYKKPKIVAGDLNTPVTFFEFQPNKGPEPGDEEKKE
                     LYFCTALVYNPSMKDRDILGTKEGVTIKIRDPHQSYIPTNKHKVRILDYRYGDKVWEI
                     IDVSPDLEENSIVKIVLELIT"
     gene            9346..9750
                     /locus_tag="IWO89_001334"
     CDS             9346..9750
                     /locus_tag="IWO89_001334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0456101.1"
                     /translation="MSNVKFEGVQETIQALEKKYGEKKTKTLTKKAINVGAEKVEKQL
                     QTDMTVFKDKGYTIAEVVRKNATYRDYKAEAEIGWNGPHQRYRIIHLNEWGYTRKGKQ
                     IRPRGFGVITKSLKNSELLYFDAVGEEVKKSL"
     gene            9747..10157
                     /locus_tag="IWO89_001335"
     CDS             9747..10157
                     /locus_tag="IWO89_001335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0456102.1"
                     /translation="MKDMLNIIYEALCLNEYIHSMTYNENTEQYRIKFYEQPETADKT
                     GPMITIRPVDTPNEAYHGSNKELSVEHMIQIDVESKYRATCKQIQYEIKKEMKKLGFG
                     QISGQGLDEYFTETNRFVDARRYEGNTKIYDTEY"
     gene            10218..10805
                     /locus_tag="IWO89_001336"
     CDS             10218..10805
                     /locus_tag="IWO89_001336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HAQ0456103.1"
                     /translation="MTLVGFKKMTIGVFDSTGKIPAANLYVIEGEQNKGATVSAEISG
                     LSKEPSKVYGSNIAYYVSQKGTGDVSATFGLLDLPTEVNDKILGYKTDTNKISFLGED
                     TEPPYCAILMESEDLNGDTAMLAMFKGKFSRESINLNTTTNEAFEPEAEEYVFSAIAN
                     DVEGDAKGQTVAKYVGDEEASITGLKEMVFPAAGE"
     gene            10878..11345
                     /locus_tag="IWO89_001337"
     CDS             10878..11345
                     /locus_tag="IWO89_001337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig domain-containing protein"
                     /protein_id="HAQ0456104.1"
                     /translation="MPDTFKIYKKNGTEFTKVAEGESPLSITGIAANTQVAKGDYQAT
                     RVVGGQESVKVDIPAFTTLPISVTGVTLDKTTAEVEQGGTLKLTPTVTPANATDKTGS
                     WSSSNTAVATVTGGNVTVKTDAEVGGTTEVSFTTTDGGKVAKCTITVIEKAAG"
     gene            11391..11699
                     /locus_tag="IWO89_001338"
     CDS             11391..11699
                     /locus_tag="IWO89_001338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0456105.1"
                     /translation="MAKVRIELKEESGKKVYENIDTTGKDYRKALETIKKLNEDGVMI
                     WNRLDIYLDFAVGIFRDSKLTAEQILDGLPSEKVMSTLDDILGEVMGIESNPDPDAKK
                     "
     gene            11894..18628
                     /locus_tag="IWO89_001339"
     CDS             11894..18628
                     /locus_tag="IWO89_001339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HAQ0456106.1"
                     /translation="MANGKPLGNMIIKLDLDSSAFSKGLAGAKNAVNHQMKAMKSQMQ
                     VMNSSGNSLGALQAKYNGLGGVLQANEKQVELLTKAYKDSFDSNGNATASTAKYANQL
                     NQATARSASYESQMKTTVGQIARMKVETEGFTGKLKANSDVLINSGKKIEAFGKGVSG
                     IGSALTVGVTAPIVAGATAVTSAAISWESAFAGVKKTNDEVVDSNGNVVYSYSDLEKG
                     LRNLATQLPGSHQEIANVAEAAGQLGIKTENVVSFTKTMIDMGESTNMSAETAATSLA
                     RFANITQMSQKDFDKLGSVIVDLGNNFATTESEITEMGLRLAGAGKQIGMSQGEILGF
                     ATALSSVGVEAEAGGSAFSKVMIQMQLAVEKGFGAFDQLKQMAEEQGVPWVNLVNAVR
                     DGGKSLKAVSEQMGFTSSDLKKMYKEADNSKSSLENFANVAGVTSEQFQKLFKSDPSE
                     AIIKFIQGLKDSEKHGTSAIKVLDDMDIKEVRLRDSLLRAANASDIFKGAIEKGNGAW
                     KQNTALTNEANKRYETTESKLKILKNEVIDTAIDLGGPFVDALRDGLNVSKPLIKNLG
                     NMAKAFSAMSDDQQRNIIKWVGLAAAAGPALKLLGTGTSVIGKVTSTTGRLTKSLVDL
                     AAKAAEKKAMDAFASSVTTAGAAAAKTAGAGGIGGLVPMMGKTASAASTAAGASGIGA
                     MTTSLGLLGPALLGIVGVGGALAVGYGAWKLFGEEAWNSSQRVKQWGTDVGEEIDSTL
                     DGVQKNIEGANGQFGLLKEGFTVDDANNMAANFATAGTSLETSLTGRIKALDDALKGL
                     PQSVQDAMKEVAESEEETLGQSLSKIKDNNEEIKKIRERAANQNRDLTVSELQQIQDL
                     MKGTSQAYVDTLDVSAKDRKKILSAMNGDVAQATEEEAKTWLQSLAKQKAATTENYRQ
                     MLEEKKAALKEKGVSKEFIDALEDEMNDYIKTTNQGFDSQIAAITNKYPQLINEVSLS
                     NGQLFSKTAAAMDNTGQYTKVFLESNEKILAQAKKTSDEIADNAEKNAEQLGMVADEA
                     THAGSTWNGLVLDEKTGKVKTNVQEVVNEATKDFTKWNELKPVVHDAKLTTNAKDVIS
                     IAAVQNGYWDDMEWKEKEALLEDECSKNVYKALENAGKWNELDIPSKEAILTSNTPEV
                     MGETLMNLGLWDTYKLQVKDLDLNAYKVYDAIDGSEEKLNSWAQLDNPTKELLLDNSD
                     FSTKLFNSEEMFKRWNQLSSNAKFLSANNTKFTEVLLSSDRMWNQWVNLPDNEKRLLA
                     DNEDLATTVLSSEEIYGRWTQLPDNQKYMLADNTDLANKIFASEESWNAWKAIPDTVK
                     NLLGNNIDLLAKVKDGTVSVEEYNKVMPLLKTLYGDNVNLKEAISGAKSSIENYNKNI
                     FPSDKTAVGHDEASQAAKDAFEAFNIFQTKIPDKITKTVSADFIGPMPKNAKGTNFHP
                     GGAAMVNDQKGPTYEELITLPSGETFIPKGRNVVLDLPRGSKVLNATKTKRLVPKYAD
                     GIGNITTVSSTLNLDALILAINELTTVLRTQLMAGTTDAKDTKLSDETKVADPIIPDS
                     LSEKSDQYLGIGAQWLTNLMNGWNSAVPQYMNSEMVFITNYLNALKLQNNPNYTQGAT
                     WNKNLLNGWNSLTGTFIATINSFCNQAMVTLRNYNTPMYNNGRTWQQNNLNGWNSLYG
                     SFIARVNQLGNDSINNLRSKSGGFYNAGTFLLQSLINGMNSLGNSLSTTMNNVANTMV
                     GGIGKGVNGVISGVNYVLKEVESSKNIGNWPIPQYAKGTDGHPGGLAMINDQKGLVHE
                     EYVQMPDGRSFIAKGRDLLVNLPKGAQVLNASLTKKLKERLNIPHYEKGIGNLDIVDL
                     LDDEKRMLEFLTSKVDFSGINEPWLDMTKSGTSLMSKAANTMLQSKLSEFFTHGNFDG
                     AVNANGVYQYLVDVAQKVMGKFPGLTVTSGYRAGDAYYHGKRQAIDLAYPGISGDPRY
                     TAAANYAFEKFPSKIAYVITNGRVRDRMGLSGTRSSGQWTNWPDGDHFDHIHLNGSMG
                     SGDIFNGGVGGSGVARWRSYVSKALKMNGLPATAAYINAWMSQIQTESGGNEKAIGGN
                     DGLAEGNATGLLQTKPGTFAANAFPGHGNIMNGFDNMLAAINYAKKRYGVAGMLQVIG
                     KGHGYANGGLITKDGLYRAGEGNKPEMVIPLTRKTRAIELMGQALAFLSGSNKKETKS
                     EINSVDNRHILQAIEKQSRMTNDLLSLLLSFFKSNNRSDKELALDIQKILIRRT"
     gene            18632..19504
                     /locus_tag="IWO89_001340"
     CDS             18632..19504
                     /locus_tag="IWO89_001340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0456107.1"
                     /translation="MRTVILRNKLNEEIDLSTEDYFATEMSNLGFEVTKEHIGQWGSF
                     REIGESIELSEFQSSVMISVHGFREQQLYGSLVEFLANGPFELEFSFDSETMIRKCSL
                     KSLSKTEIDSQSSLLTDTLNLYFTSSWYSIKREKLIQNSTSLNTRGKVYSFVRPYVYT
                     KNVWEKKGVFKFSNNSVYLTNSTERMSPLKIRIIGECSNPYWEVIQNSKVVASDGYFI
                     NMKDTQILEVSSLLEDQTAILKDVGGVESSVYQQQDFTKSNFVQAPLGEFSVVFHVGH
                     ANVEIELYEERDLF"
     gene            19501..20128
                     /locus_tag="IWO89_001341"
                     /pseudo
     CDS             19501..20128
                     /locus_tag="IWO89_001341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176402.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            20089..20550
                     /locus_tag="IWO89_001342"
     CDS             20089..20550
                     /locus_tag="IWO89_001342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0456108.1"
                     /translation="MQSPGPGNENKLLIVDKAMKDIENPLVLSTWYLDDENNLTQDST
                     NKNIALPTVNLVNIYDQTQEDRPSYEEVAKSELKGNAYSHEIKVNVVLNARNINVENI
                     ETGMLATIVYKEQTYKSVLTAWRISSSKKNIELTFGNVRSRFMDYFEDNGG"
     gene            20554..21378
                     /locus_tag="IWO89_001343"
     CDS             20554..21378
                     /locus_tag="IWO89_001343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0456109.1"
                     /translation="MVSNVDGYQFENVKVSAENDARLYHVLYNRKNQVIDGYDQSMNL
                     SSSGLTVKVAAGAAIIQGRMVVVRQEESITVPANSSGYIALTVDLTQEVIPGSILPES
                     EEYEWTNNQVKLEFITKVIKGNLNNGDKVYNLPLCSVNSTGSTVSISKISDSYELTLS
                     KGEILWRGTALMHDTQTVQPSKKIWQTVSGFLLVWLPYENGQAIEDRYVTTPFYKERI
                     TFTNVLGEIVSGFDDYHKKWFSKRINYNSNTNIFTGAASNASGDNANMVLRYIVSF"
     gene            21396..23438
                     /locus_tag="IWO89_001344"
     CDS             21396..23438
                     /locus_tag="IWO89_001344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BppU family phage baseplate upper protein"
                     /protein_id="HAQ0456110.1"
                     /translation="MANLEIKLSANKRQPYLRHRVVGRVGDGGLTTINVQLLEEDEIT
                     PFVINLNGTLKFVGEVSNGNYTEGEPEIIDSTNGLISYTFTKSNFSTSHQFKQAYFEY
                     VDPNGKKVTFQNFIIDVLERVDINSEQAKYYISSLEKLQSEMQTTFNQFISDKQVQYD
                     QIYSKYNELVRLINESDKQVNDRIDKANNRIDQTNQQIGDLGKLKKMYSNSIDFGGYD
                     YSGKPNLMSKLKSSDFNVGYHGSLTSDNEKLHFTSDGTGSIIMFTRINTPQLASGKTY
                     TLSAKVRFDEGTTGAIDKLRLVYRTSPGEKILLEANSTNITTDDVGKEITIKGTANVN
                     YQITNLDRFYMSISFVDRDKINGGFKLYDIKIEEGSTATPYQPNLLDDPYWVGKTSLG
                     ENIANKGTTFPIKSSAYEIYKGNTEEELIIGQTYTITLKGTKPASQTFVAYNYWNVNF
                     GELKPVEGLTDVWSLTFTPTKLEPGLPKDLRIFQLPKETAGACQIDWLKIEKGDTRTP
                     NISQFKYFGEGLKDSNNPNDYSWDITPEYTEKGLNDTVSLTEPQSIEGLKNFEDGIQS
                     KGKSVLTSDDNKYEVVTLTVTNGNTGSAKLYREGKTVTIYFFALNGKSSGGNDSTILT
                     IPEGYRPPISFEQLVGSIDRSTLNSAQLSIGADGAIKWRRNSSYGSAYSFVITYSI"
     gene            23598..24044
                     /locus_tag="IWO89_001345"
     CDS             23598..24044
                     /locus_tag="IWO89_001345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0456111.1"
                     /translation="MKNIWKYGRTGGEYAGKVLDDMLVSVPYTDQPPLEGVRSDGEPL
                     TIADQMFDPKLNQWIILANALDHNDLNNLKAMYEALEHENGNLKQLNAKLMLSDVAIK
                     QENTALKEKADSLAQINSKMMLASLQNSKDISEIKEQLNPASKGGE"
     gene            24046..24183
                     /locus_tag="IWO89_001346"
     CDS             24046..24183
                     /locus_tag="IWO89_001346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XkdX family protein"
                     /protein_id="HAQ0456112.1"
                     /translation="MFSFSDVKMMYDWGCFTDDQVRLFVPLCITDEEADKIINKDKSA
                     S"
     gene            24220..24513
                     /locus_tag="IWO89_001347"
     CDS             24220..24513
                     /locus_tag="IWO89_001347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0456113.1"
                     /translation="MINLGEWGMIAGSITAIVSLILLVIRPIAASFSKITETLSKVSH
                     NLDLLTKDLESSKSDRLMIHEELKKHDERLDTHTEKLVEHTQQIKTLFRERRK"
     gene            24510..24734
                     /locus_tag="IWO89_001348"
     CDS             24510..24734
                     /locus_tag="IWO89_001348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="holin"
                     /protein_id="HAQ0456114.1"
                     /translation="MNNKTFEVLKWFALVIIPALATFVGLVGKALNWQYTDICVVIIT
                     GFGAFLGSVLGVSNRTYKMFSAESEEGGNK"
     gene            24731..25750
                     /locus_tag="IWO89_001349"
     CDS             24731..25750
                     /locus_tag="IWO89_001349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HAQ0456115.1"
                     /translation="MKKKITITAMSLLTALFLLPINGFAYTINNEFNLGANEGSSQVA
                     NNQYILLHETANETATGRNEAQYMQRSWTSAYTAYIVGDGGIVYQVGQPGYVQYGAGS
                     YANANSPVQIELQHTHDKATFEKNYKAYVELARDSAMKYGIPLTLDTPYNQPGIKSHL
                     WVTQNIWGDHTDPYGYLSEMGVSKEKLAYDLAHGFTDENPTTSENKPVIDPTRAGAAN
                     PTLSDGTNHSHIDQFGEIENANLHVAGWHIANYKYEYIFIMDYNTGKELARVRADGIY
                     RPDVNQAYNTSGNVGYHVSFNMRNFPNKKVYVMMRATNDPEGNTKGGAQDFHDKRWYL
                     NIPKR"
BASE COUNT         9217 a         3965 c         5463 g         7204 t
ORIGIN      
        1 tccttctaaa ttgttcttat tatctatcat cagttgctgt ctattgtcat tatgtcactg
       61 tggtggaaag gtgtatcgct catctaaaat ttaggtgcaa actgcaaggt tcgattcctt
      121 gccagtgact ttggtaaccg aggcatcggc ggtttaaaaa tataggggtg cgcaatttcg
      181 tacgcgtttt gtgcatcttg taggttgcta ttacataatt ggttgggtta gattgagttt
      241 tgggattcgg tacaaatgaa tcgtcaaatg actcaagcac aggatcggaa acgtccctgc
      301 ctgtgcatta catattagat cactctttga gtggtctttt tattttaaaa aagagaggat
      361 tttgaaaaat ggaattgatt gtatttacta ataacggaca aacatatcat ttttatgaag
      421 tggagaattt taagccaaca acaacaggat ttagttttat ttatactggg aaggctacgg
      481 gagttactcg ttcagctacg tttaacaata catcaacagc tggttatgcg cttgcggagg
      541 tacaaaatga atcatgagaa gtttatcgaa aaatgcaagg ctattgtacg tgaaagaatt
      601 gaaaatgaga ttgctgacct aagtggagca gtgcctgagt ttagtatttt catagtctgg
      661 tcatgcaaaa cactgcaaaa tagcaaagca ttagtcagcg ctagcttaaa aggagcaccg
      721 tattttgaaa ttacgttgaa cggggacaaa ggtgaaatct atgtagatac ttatctcaaa
      781 aaatcaaatg aatgtatcaa agtctagcag gtgctaggct ttttctttac ataaaggagg
      841 ctgcataatg agaaactact ggtatgtatc actaactaat gaatatcctc gaaccattgg
      901 tgattgttca gtgcgtgttg tgcgttctgt acaaatcaaa gggaagtact ctattgtcga
      961 aatgctaaga gaagctacac caaacgaagt ggataaatgc aagctgatat attgcggtca
     1021 tggctattgg aaagacgagt atatccaata caacattgag aggtacatca aatgaatgat
     1081 aactatgatt acatcaagtt gttagcaagt aattattaga tgtttttagg aggaaaacta
     1141 tgaaattaaa agattatatc agagaagggt ataacgttgt aactgcaccc aatctagctt
     1201 acaaaattca agaagattat cccaatgctt tagtgtttac tgatagagct ttagatgcca
     1261 ttcctatagg gaagttatta gtagatcgtt tttatagcaa taatccagct gttcttagtg
     1321 ctaagccact tcaaaacgct tatgctattg aacgctttac ttgcgagttt actggatatg
     1381 agacggtagt accaatgaca ggtgagacag gaagaaaagt aattcaacag atcaaaaaga
     1441 ggattgctga attgacgtta gatgatggaa gtaatacgaa ccttctagaa atcaaactaa
     1501 gagatactga ctcagtacca gaagtttatt ataaaggtca acgaatggat gagatgccga
     1561 taggtcttgt cgatattact tatcattggg atacggaaga atatgaatgc caagtaccaa
     1621 aaggagctaa cgacatcact attgaatatt atgattcaca taataacaag tacttagata
     1681 gaaaaatcat tgggcacaag agagatatgt aaatgattga tgtttcaagt aaacaagcaa
     1741 gagctaggtt ctatggatca actgagtgga ggaaacttag aagtcaatgc ttagagcgag
     1801 atcattatga gtgccaatgg tgtaaagcag agggtagact aacaactcaa catgattcaa
     1861 ttcttgaagt ggatcatata aaggagctgg agtatcatcc ggatttagct ctagatataa
     1921 acaacctaag aaccttatgc aaagattgcc ataacaaaag gcacaaccgt atgaaatatc
     1981 gtggccaaca aaagaaaaga aagtgggatg atgaatggtg gtaagaaaaa cagcaaacag
     2041 agatgatgtc aaacgagcag aagcaatcgc tatggttgat aaggctattg ataagttggt
     2101 tctatttaag agtcagctca ttgaaggaac aacaactgta gaggatatat ctatcaacaa
     2161 tagactaata ggtaacgcta atgaagtaac agggacattt aatattaatg tagacattga
     2221 ttataccttg agtccatttg cacatattgt agaaagcaac gagcaagacg gctgataaat
     2281 cagtataaag cgaagatata tagagtgaaa tacccccggt caaaagattt tgagaagaaa
     2341 taaaattttt gggaaccggt ggatggggtc aactctgcaa ataaatgatg ctattttcac
     2401 acgaccccct ccccacgtag aaaggaagtg agttgatggc tgatctgcaa gagagaaata
     2461 ggcttgtttc tgctgaagaa aggcgtctaa ataaattgtt taaggatatt gataaggata
     2521 aaaagaaggt cgtttctggt ttgattacgc aagcggcacg cttaaaaatt cttcttgatg
     2581 aaatgtggat tgatatttcc gaaaaaggtg attatgagct tttctctcag tcagaaaatc
     2641 aaaaacctta cgagcgggaa cgaccagttg ctaaacagta taattcgaga gaccaagcat
     2701 atcaacgtgt aattaagcaa cttactgact atttgccaga agaaaagcgc gaacctacaa
     2761 aaaatgcagc gctagatggt agtgatttgc tgtgacaaca ttaaagccat tctattttga
     2821 tgaatacgta gatttatacg aaaaaggggt aatccctttt aataaagaaa gaattcagtt
     2881 aattacttat ttaaaaaacg aagtgctagt tcgggatgat atctattttg atgaagaaat
     2941 gattcaaaag tttatcaagt acactgaaaa aaattttttt cctttagcta aatatcaaaa
     3001 atttatcaca cctttcattt tctgctatca aaaagaggcc gacgaggtgt tttttgatga
     3061 aattctaaat agtattgcgc gaggcggcgg gaaaaatggg ttcatgtcag caagagactc
     3121 attttttatt agccctctct acggtgtcag aaattatgat gtaacgatta cagcgaattc
     3181 tgagaagcag ggaaaggtat cttttaaaga agtttatgaa atggtacaaa tgcgcaaact
     3241 ggaaaaacaa ttttatttga ctaagatggc tatagtcaac aatgtaacta attcgatttt
     3301 tagttatagg acaaacaatc ctaagactat ggatagtgca agagatggct gcttagaatt
     3361 tgatgaaatc catcagtttg aatcttcaga cctagttgat attcagcgta gtgggctagg
     3421 gaaaataaaa catcctcgaa ctttttataa tggtacaaat ggtcatgtta gagaaggttt
     3481 ttatgacaaa atgttggaac gagcacaaaa gatttttagt ggagagaaca agaatgatcg
     3541 cttgtttccc tttatttgta aactggataa cattgatgaa atggataatc ctaaaatgtg
     3601 gtcaaaggct aatccaatgt tcgaagaaga ttcagcatat tcaaaacgtc tgttttctac
     3661 agtgatgaaa gaatataaaa aactagaaga tgagccatca ggtaggcgtg aatttgttgt
     3721 taaacgaatg aattttacag aaggtgatgc agaatcagat attacgactc atgaaaaatt
     3781 aatggctact aaacaagaat tgccaaattt gaatggtcgt tcttgtgtgg ctggatttga
     3841 ttatgcaagt attcgtgatt ttgcaacagt gggtttgctt tttaaaattg atgataaatt
     3901 tatctggcag caacatagtt ttgctaggaa aaagtttcta gatacattca aactgaaagc
     3961 gcctattaag gattgggaga aaaaaggttt agtgacaatc gttgatgagc catcaattga
     4021 tcctcgtcat ttaattgatt ggttaaatga aaaaagaaaa atccatcaaa tagaaatcgt
     4081 ttgtgccgac ggatttcgta tggatttgct gaaacctctt ctagagaaag aatcatataa
     4141 ttttgagttt atcagaaata taagaggtgt gcagtcaaaa gttgctccta ttattgaaga
     4201 tggttttgca aatgaacggt ttatttttgg tgatgatcct atgatgcgtt ggtatacaaa
     4261 taattcgttt atcaaagaag acaaattagg gaataaaaca tttttgaaaa aagagccagt
     4321 aaggcgtaaa acagatgggt ttcatgcttt tttggcagct ttatataaac gagaagaaat
     4381 aactgatatg gattatgagg aagcatttga tatgttggat gagattgaat tttaaggtat
     4441 actccttatt gaaaggggta gttatagaag tgaaaaagag atattttcta tttgttacaa
     4501 tatttataat aatagcaatt tgtgttttga cagtattttt accacaaatt actgagtatt
     4561 taaattatcg gatatttacc acaaaagtag atgatagcgt tactagttta agtaggctag
     4621 aatttataaa aatatgtgca tctgtgtttg gtcctatttt gactgtaatg gtatttgtga
     4681 atactttaaa tatgcagaaa aaaacagagc aaaagcaaaa tgagcttgaa gtaaagcaag
     4741 ctaaggctga gaacgagaga gcaaaaaaag agctgttaac acagatagat cgtgagttct
     4801 attttttatt aaatatgttt attgaaatac aaaaagatca aaatgccaaa caagctgtta
     4861 ttgctttaca gaagttggca atttttaacg aaaagggttt taatcaagac tatgcattta
     4921 cttctaaaga tttatcagat agtgaaattg ggagtacaat tgaacaatca ggaccgctga
     4981 agggcacagt tgaatatata aaatttgaaa catttgatgt aagcaagtcg atggggaatt
     5041 atcaaacaaa aacaaaagac gttgctacaa gtttacacga tcagaaagta gctatattga
     5101 atgaacaata tgaaaagatg caaatatatc ttggacggta cttcaaaatg tttcaccgaa
     5161 tagttaaaac attaaatgag tattatgacg attataatga ttttgatgtg aagaggtata
     5221 caaaatatat agggacatta agaacacaaa tttctccagc tgaattccaa gtgattttat
     5281 tcaattctct atatataaaa cgaggttttg gcttaggaat tcaacttatt ggaagtggtt
     5341 tttttggaga tgattttgat tttgaaacta atcaacattt tgaaacaagt atcaatgaac
     5401 agtggtttct atcactatca actgttgaca gtgataactc tataaagcga caaaaattat
     5461 ctgaatatat taaagaatta ggatccgtag aatataaaaa aatagctaat tttgaaagtt
     5521 tatataaact ctttaatgaa actaagctct agaaataggg cttttttatt tttctttgaa
     5581 aggtggtgaa aacatgtgag cttatttgat atctttaagc agtcaataag aaatgaagag
     5641 ccttctgagt ggataccgga ttttgtatat ggagaggaag tatctgctcg ggcatacctc
     5701 aaaattatgg caaaaaatac tgttttagat tttgttgcaa gaactatgtc aacgttagaa
     5761 gttaagttta aaaataaaga tgggacatca gactgggatt atattctaaa tgttcgacct
     5821 aattctgata tgtcagcagc tacattttgg caaaaatttt tttatcggct cttagatgac
     5881 aacgaagtgt tagtaatatt tacagatgat aatcaattgc tgattgctga tgattttagt
     5941 cgaacggagt atgccgtgta tgatgatgtt tttacaaatg tgatggtaaa aaattttgtc
     6001 tttgaacgtt catggaacat gtctgacgtc atttatatgg aatataataa cgaagaatta
     6061 gaaaggttta cacgaggttt atttgaagat tacgccgcgt tgttcggacg gattctagaa
     6121 gttgcaatgc gtaacaacca aattcgtggg tctgtatcca ttgattcaac gggaacggcg
     6181 aatgatgaaa aaggaaaaga tggtaagact agatctcaaa gattacaaga atatattgat
     6241 aggatttgga actcttttaa aactaactca gttgcaattg ttcccaaagt gaaaggcttt
     6301 gagtatgagg aatacaccaa taaacaaggc gtatcgaatc aatcgctaga tgaactgaat
     6361 aagatgaagt cgtctttaat tgatgatgtt gccaacgcca taggagtacc tacggcgctt
     6421 atttatggtg aaaaagctga actggattca aatattaaag cttttcgtaa attgtgtatt
     6481 gctccactag ttaaaaaact tcaagacgaa ttggtggcaa aaataattag tcgtcaagag
     6541 tacaaaaacg gggaacgtat agaagttaaa aaagttctcc aaaaagatct attagagtat
     6601 gctactcaaa ttgataaaat aacatctggt ggtgcgtttt atattgacga agttcgtgat
     6661 gaagttgatt atgatgagtt gccaaataat gaaggaaaaa ttcgtattcg tacgaaaaac
     6721 tatgaagaaa ctgtgaaggg aggtgagaac gacaatgccg aaagctaaaa aagtaccatt
     6781 tcagtttact aacgaaattc agaatggtaa gcacattctc accttgagtg gaaatgtaca
     6841 gaaaaaatat tggcgtgatg atgatgtcat aaacgctaaa gatattcgtg actctctaga
     6901 tactgtaacc gatgatattg taatcaagtt gaacagccca ggcggtgatg tgtttgaagg
     6961 gattgagatt tacaattatt taaaagatca cccatcaaat attactgtag aagttacagg
     7021 gcttgccgca agcgcagcaa cgtttattgt cgctggtgcc aatgatgtga ttatgaatgt
     7081 tgggacttct ttgatgattc atgaagcttc tacatttgct tgggggaata aacaggacat
     7141 ccaaaaaacg ttaaacgcat tggaaacgat cgatgattct atcttagcta tttattcaga
     7201 taagactggc caatctgttg ataagttgcg tgaatggatg aatgaagaaa aatggtttac
     7261 agcagatgaa gctgtcaaat atggatttgc caattctgtg aagcgtgaag aaccacaaaa
     7321 tgaacctcaa aatattgcag cgatgattca agatgctgtt gccgttgcaa tagcgaattt
     7381 aaaggaacct gttactaata aaatcgaaca agaatcaaaa caaaaatcat taatcgcacg
     7441 attgcgaaaa ggagaataaa gatatgttaa aaattacaga taaaactgca gatgcaaaaa
     7501 aggcatttaa tgctatttct tcgaaagaag atgcaacacc cgaacaaatt aatgctgctc
     7561 tagaagaata tgtaaccgcc attgcagaag atgccggcaa acaggtgcgt gctgaatatg
     7621 aagaattgaa aaatgtgacg gacaaccgtg tattagaggc tcgtggaatt cctacactaa
     7681 cagcagaaga aacaaagttt tataatgaag ccgtaaaatc tggaggtttc gattctgacg
     7741 tagtttggcc agaaacgatt ttggaacgtg tttttgaaaa tcttcaaaac gatcatccta
     7801 ttttaggcct cattaacttt acaccaactg taggtcgtgt caaagtaatt agagcacgtc
     7861 gtaaaggtgt tgctgttttt ggaccattgc ataaagacct tgaaggccaa ctagatgctg
     7921 aatttggtgc aactgaattt gttcaacttg cgttgaccgc atttttctta atttctaatg
     7981 ataccttaga tttaggacca cgatggattg accgttttat caatctttct ttaagtgaag
     8041 cagtacgtga tgtttgggct aaaaaaatta ttacaggttc tggtaaagat gagccaattg
     8101 gattgcttaa agatctagat ggtgcggtca caagtggtgt atatccagat aaagctagcg
     8161 cagggacatt aacttttgcg aaagagaaga ttgttcttga acttgcaggg gtgatgaaaa
     8221 aattatctaa atacacatac aaagtagatg gtgacgatga aggtgaagtg aaataccgtt
     8281 ctgttgctgg acgagtgtat ctaattgtta atcctgttga ttactgggat atcgtagcta
     8341 gagttacatt tgcaaattta aacaatgtgt atgggactac gatgccattt atcgctgttg
     8401 atcatatcat cgaatctgta gacgtaccag aaagtaaatg tattgcatac gttgataaag
     8461 agtatgaagc tactcaatca agagctgaaa aagtctatgt atataaagaa acttttgcaa
     8521 tgaaacgcgc aactttgtat gctgtcgaca tgcttggtaa tggctatcca acaaataatg
     8581 atgcagcaca agtatatgac ttggacttta cagcggggga gtaatgtccc ctgcaattgg
     8641 tgagataaca ccgacgactg atggggcagt cattaatctt tcgtaaatgg ggttagagta
     8701 tgaacactga agaatttata aaagaatata aggcccgatt tcgtattttc catacttccg
     8761 aagatgagga tatcgggaaa cagttagaga gcgggtttgc cgatataaaa tctctaatcg
     8821 gcagttttga tccttccact tatgagaaag gaaaagaatt agtttatgaa cgtactcggt
     8881 atctacggaa cgaatcacta gagtactttt acgacaattt tcaaacaatg attatggatg
     8941 catcaattga tctggcaggt gctggatatg ccaattaaat caaattataa gaagcctaaa
     9001 attgtagctg gtgatctaaa tacgccagtt actttttttg agtttcaacc taataagggt
     9061 ccagaacctg gtgatgaaga aaaaaaggaa ctgtattttt gtacagcact agtttacaac
     9121 ccatcaatga aagatcgtga cattcttggc acaaaagaag gcgttacgat aaaaatacgg
     9181 gatccccatc aatcttatat tcctactaac aaacataaag taaggatatt agattatcga
     9241 tatggagata aagtttggga aataatcgat gtctcaccgg atttggaaga aaattcaatt
     9301 gtaaagatag tattagaatt aattacttaa atttggtgat aaatgatgag taacgtaaag
     9361 tttgaaggcg ttcaagaaac aatccaagct ttagaaaaaa agtatggaga aaagaaaact
     9421 aagacattaa caaaaaaagc aatcaatgtt ggcgctgaaa aagtagaaaa acagttgcaa
     9481 acggacatga ccgtttttaa agacaaaggt tatacgatcg ctgaggttgt tcgcaagaac
     9541 gctacgtata gagattacaa ggcagaagct gaaatcggtt ggaatggtcc acatcaacga
     9601 tatagaatca ttcatttgaa tgaatgggga tatacacgaa agggcaaaca aattagaccg
     9661 agaggatttg gtgtaataac aaaatctctt aagaattccg agctgttgta ctttgatgct
     9721 gtaggagagg aggttaaaaa gagtttatga aagatatgct taatatcatt tacgaggctc
     9781 tttgtttgaa tgagtatatt cattcaatga cctacaacga gaataccgag caatatcgta
     9841 ttaaatttta tgagcaacct gaaaccgccg ataaaacagg tccaatgatc acaattcgtc
     9901 ctgtagatac acctaacgaa gcgtaccacg gaagtaacaa agagctttct gtggagcata
     9961 tgatacagat tgacgttgaa tcgaaatata gagcaacatg caagcagatt caatatgaga
    10021 taaaaaaaga gatgaaaaaa ctaggctttg gccaaatttc cggtcaaggg ttagatgaat
    10081 actttactga aacaaaccgc tttgtagacg cgcgtcgtta cgaaggaaat acaaaaattt
    10141 acgataccga atattagaaa ttaagacacg aaaataacgt gtcttttttt attacccaaa
    10201 attaggagga aaattttatg actttagtag gattcaaaaa aatgacaatc ggggtctttg
    10261 acagcacagg gaagattcca gcagcaaatt tatatgtaat tgaaggtgaa caaaacaaag
    10321 gggcgactgt atctgctgaa atcagtgggc tatcaaaaga accatcaaaa gtttacggat
    10381 cgaacatcgc ttactatgtc tctcaaaaag gaacaggcga tgtttcagct acttttggat
    10441 tgttagattt accaacagaa gtaaatgaca aaattcttgg ttacaaaacc gatacaaaca
    10501 aaatcagctt cttgggagaa gatactgagc caccatattg tgcaattttg atggaatcgg
    10561 aggatttgaa cggcgataca gcaatgcttg cgatgttcaa aggaaaattc agtcgtgaat
    10621 ctattaatct taacactacg acaaatgaag ctttcgaacc agaagcagaa gaatatgtat
    10681 tttcagcgat tgccaatgat gttgaaggtg atgctaaagg ccaaacagtc gcaaaatatg
    10741 ttggagacga agaagcttct attactggtt tgaaagagat ggtatttcct gcagcggggg
    10801 agtaactagc cccgttgttg gagcaatcac tcccactatt aatggggcaa cagttgaatt
    10861 aagttaggag gataactatg ccagatacat ttaaaatcta taaaaaaaat ggtactgaat
    10921 ttacaaaagt cgcagagggt gaaagtccac tttctattac tggaattgca gcaaatactc
    10981 aagttgctaa aggagattat caagctacgc gtgtggtcgg aggtcaagaa tcagtaaaag
    11041 tagatattcc tgcattcacc actcttccaa tttcagttac tggtgtgacg ttggataaaa
    11101 cgacagctga agttgaacaa ggcggaacct taaaattaac tcctactgta acaccagcaa
    11161 atgcaacaga caaaacagga agctggagca gttcgaatac agctgtagct acggttactg
    11221 gaggcaacgt tacagtaaaa acagacgctg aagttggcgg aactacagaa gtttcattta
    11281 ctactacgga tggcggtaaa gtagcaaaat gtactattac agttattgaa aaagcagcgg
    11341 gctagtcttc ggactagctt ttttatttgg aaaataatgg aggaatctaa atggcaaaag
    11401 taagaattga attgaaagaa gaatcaggaa agaaagtcta tgaaaacata gacacaacag
    11461 ggaaagatta tcgaaaagct ttagaaacaa tcaaaaagct aaatgaagac ggagtaatga
    11521 tttggaatcg tttagacatt tatttggatt ttgctgtagg tatcttcagg gatagcaaat
    11581 tgacagctga acaaattctt gatggcttac catctgagaa ggtaatgagc acattggacg
    11641 acatcctagg agaagtaatg gggattgaaa gtaatccaga cccagacgca aaaaagtaac
    11701 acccgaagaa gcagaagaaa tgtacttaaa cctatgcaga gagttagtga aagcgggatg
    11761 gtcactatct gacatagaag ataattcctt tgaaatgatg atgaaaattg cgtgcacttc
    11821 gcctaagaaa gagaaacaaa aagaagttga tcttaaagac ttcttgaaat ccatgtagag
    11881 aggaggtaaa catatggcaa atggaaaacc tttaggcaat atgattatca aactagactt
    11941 agacagttca gctttttcaa aaggtctagc cggagctaaa aacgctgtta accatcaaat
    12001 gaaagcaatg aaatcacaga tgcaagtaat gaactcttca ggaaattctc tcggtgcttt
    12061 acaagcaaaa tacaatggtt taggcggagt tctacaagca aatgaaaagc aagtagaatt
    12121 gctcactaag gcttataagg atagttttga ctctaacgga aacgctactg cttcgactgc
    12181 taaatacgct aatcaattga atcaagcaac agctagatca gcaagttacg aatctcagat
    12241 gaaaacaact gttggacaga ttgctcggat gaaagtggaa acggaagggt ttacggggaa
    12301 actaaaggct aattctgatg tattgattaa ttctgggaaa aaaattgaag cctttggtaa
    12361 aggagtttct gggataggat ctgccctaac tgttggagta acagctccaa tagtggcagg
    12421 cgcaactgct gttactagtg cagctatttc atgggaaagt gcttttgcag gtgtaaaaaa
    12481 gacaaatgat gaagttgtag actcgaatgg aaacgtagtt tattcctata gcgacttaga
    12541 gaaaggtctg cgtaatcttg ccacccaatt acctggtagc catcaagaaa tagcaaatgt
    12601 agccgaagca gccgggcaat taggtataaa gactgaaaac gtagttagct tcactaaaac
    12661 gatgatagac atgggtgaat cgactaatat gtcggctgaa actgcggcaa cttctttagc
    12721 acgatttgct aatattaccc aaatgtctca aaaagacttt gataaattag gatcagtcat
    12781 tgttgatcta ggtaacaatt ttgctacaac cgaatcagaa atcactgaaa tgggattgcg
    12841 gttagcggga gctggtaaac agattggaat gagccaaggc gaaattctag gatttgctac
    12901 tgctctatct tctgttggtg tcgaagctga agcgggtggg tctgcctttt cgaaggtcat
    12961 gatccagatg caactagctg tagaaaaagg atttggcgct tttgatcagc tgaaacagat
    13021 ggcagaagaa caaggtgttc cgtgggttaa tttagtaaat gctgtgagag acggtggaaa
    13081 atcgttaaaa gcagtatcag agcaaatggg attcacttca tctgatttaa agaaaatgta
    13141 taaagaagcg gacaattcga aaagttcgct tgaaaatttt gcgaatgtgg caggtgttac
    13201 aagtgaacaa ttccagaagt tgttcaagag tgatccttca gaagcaatca tcaagttcat
    13261 tcaagggctg aaagattcag aaaaacatgg tacttcagca atcaaagttc tggatgacat
    13321 ggatataaaa gaagttcgac tacgcgacag tttattgaga gctgcaaatg ctagtgacat
    13381 attcaaagga gcaattgaaa aaggaaacgg cgcgtggaaa caaaatacag ctttaacaaa
    13441 tgaagctaat aaaagatacg aaacaactga atcaaagcta aagatattga aaaacgaagt
    13501 gattgataca gcaatcgatc ttggaggtcc atttgtagac gcgttgcgtg atggtctaaa
    13561 tgtatcgaaa ccattgataa aaaacttagg gaatatggcg aaagctttct cagctatgag
    13621 tgatgatcaa caaagaaaca ttatcaaatg ggttggatta gcagcagcag ctggtccagc
    13681 gctgaagctt ttaggaactg gtacttcagt aataggtaaa gttacttcaa ctactggaag
    13741 attgacgaaa agccttgttg atttagcagc gaaagcagca gaaaagaagg ctatggatgc
    13801 ctttgcatcg tcggtaacaa cagctggtgc tgcagctgca aagacagcag gagctggagg
    13861 tattggcggg ttagtcccta tgatgggaaa aacagctagt gctgcaagta ctgctgcggg
    13921 cgccagcgga ataggcgcaa tgacaacgtc actaggtttg ttaggtcctg ctctacttgg
    13981 tattgttggc gttggtggcg cactggcagt tggctatgga gcatggaaat tatttggtga
    14041 agaagcttgg aattcctcgc aaagagtaaa gcaatgggga acagatgtcg gtgaggagat
    14101 agatagtact cttgacggag tacagaagaa tattgaaggt gcaaatggtc aattcggtct
    14161 tctcaaagag ggttttactg ttgatgatgc aaataatatg gcggctaatt tcgcaaccgc
    14221 tggaacatct ttggaaacat ctttaacggg tagaataaag gcattagacg atgcactaaa
    14281 agggctacct caatcagttc aggatgccat gaaagaagta gctgaatcag aagaagaaac
    14341 cttagggcaa tctctttcta aaataaaaga caacaatgaa gaaataaaaa aaatcagaga
    14401 aagagccgca aatcagaaca gagacttgac tgtttcagaa ctacaacaga tacaggatct
    14461 aatgaaagga acttctcaag cgtatgtgga tacgctcgat gtttctgcta aagacagaaa
    14521 aaagatacta tcagccatga atggtgatgt tgcacaagct acagaagaag aagcgaagac
    14581 gtggttacaa tctttagcga aacaaaaagc tgcaactacc gaaaattata gacaaatgct
    14641 tgaagaaaaa aaggcggctt taaaggaaaa gggagtaagc aaagaattca ttgatgcatt
    14701 agaagatgaa atgaatgatt acatcaaaac tacaaatcaa ggattcgata gccaaatagc
    14761 tgctataaca aataaatatc cacaattgat taacgaagtg agtttatcaa atggtcaatt
    14821 atttagcaaa acggcagctg ctatggacaa tactggtcag tatacaaaag tttttctcga
    14881 aagtaatgaa aagattttag cccaagcgaa gaaaacatcg gatgaaatag cagataatgc
    14941 cgaaaaaaat gcagaacaac ttggtatggt agctgatgag gctacgcatg ctggatcaac
    15001 ttggaatggc ttagttttag acgaaaaaac aggaaaagta aaaactaatg tacaagaagt
    15061 agtgaatgaa gcaacgaagg atttcactaa atggaatgaa ttgaaacctg ttgttcatga
    15121 tgcgaaacta acaacgaatg caaaagatgt catttccata gcggcagtac aaaatggcta
    15181 ttgggatgat atggaatgga aagaaaaaga agcattatta gaagatgagt gttctaaaaa
    15241 tgtatacaag gctttagaaa atgctggtaa atggaatgag ttagatattc cttctaaaga
    15301 agcaatccta acttcaaata ctcctgaagt tatgggtgaa actctcatga atttgggatt
    15361 atgggatact tacaaacttc aagtgaaaga tttagatttg aatgcgtata aagtatacga
    15421 tgctatcgat ggatcagaag aaaaattaaa tagttgggct caattagata accccacaaa
    15481 agaattgtta ttagataact cagatttttc tacaaaactt tttaattccg aagaaatgtt
    15541 taaacgttgg aatcagttat catctaatgc aaagtttctt tcggctaata atactaaatt
    15601 tacagaggtt cttttatcat ctgacagaat gtggaatcaa tgggtaaacc ttcctgataa
    15661 tgagaaaaga ttacttgcag ataacgaaga tttagctacc actgttttat cttcagaaga
    15721 aatatacgga agatggactc aattaccaga taatcaaaaa tatatgttag ctgacaatac
    15781 tgacttagca aacaaaatat tcgcttcaga agaatcatgg aatgcatgga aagcaattcc
    15841 agacacagtt aaaaacttgc ttggtaataa tattgattta ctggctaaag tgaaagacgg
    15901 gactgtaagt gtagaagaat ataacaaagt tatgccactt cttaaaactc tatatggaga
    15961 taacgttaat ttaaaagagg caatcagtgg ggctaaaagc tctatagaga attacaacaa
    16021 aaatattttt cctagtgata aaactgcagt aggacacgat gaggcttcac aagctgctaa
    16081 agatgcattt gaagcgttca acatcttcca aacaaaaata ccagataaaa ttactaaaac
    16141 tgtatcagct gactttatag gtcctatgcc gaaaaatgcg aaaggaacaa acttccatcc
    16201 tggcggagca gctatggtaa acgatcaaaa agggcctact tatgaagagc ttataacctt
    16261 gccaagcgga gaaactttta tacctaaagg acgtaatgtc gttttagact taccaagagg
    16321 ttcgaaagta ctgaacgcca ctaaaactaa acgtctagtg cctaaatatg ctgatggtat
    16381 aggaaacata acgacggttt catctacgct aaacttagat gctttaattt tagctattaa
    16441 cgagctaacg acggtattga gaactcaact gatggcagga acgactgatg ctaaagatac
    16501 taaactatca gatgaaacaa aagttgccga tccgattatt ccggacagtc tttctgaaaa
    16561 atcagatcag tatctaggaa ttggagctca atggttaact aatctaatga atggttggaa
    16621 ctcagcggtt cctcaatata tgaatagtga aatggttttt attacaaact atctcaatgc
    16681 gctaaaactt caaaacaatc ctaattatac gcaaggagca acatggaata agaacttact
    16741 gaatggatgg aatagtttaa ctgggacctt tattgctaca atcaattcat tttgtaatca
    16801 agcaatggtc acgcttagaa actacaacac tcctatgtac aacaatggcc gaacgtggca
    16861 gcaaaataat cttaatggtt ggaattcatt gtatggatca ttcatagccc gtgtaaatca
    16921 gcttggtaac gattcgatta acaaccttcg ttctaaaagt ggtggttttt acaatgcagg
    16981 gacattcttg ttgcaatctt taataaacgg aatgaattcg ttaggtaatt ctctttctac
    17041 tacaatgaac aatgtcgcta atacaatggt tggtggtata ggcaaaggag ttaatggcgt
    17101 catatcggga gttaactatg tgcttaagga agtcgaatca agtaagaata tcggaaactg
    17161 gccaattcca caatacgcta agggaactga tggacatcct ggtggacttg ctatgattaa
    17221 tgatcaaaaa ggcctagttc acgaagaata tgttcagatg ccagatggac gtagttttat
    17281 tgccaaaggt agagatcttc ttgttaattt gcctaaaggt gctcaggtct taaatgcttc
    17341 tttaacaaag aaattaaagg aacgtttaaa tattccacac tacgaaaaag gtattggcaa
    17401 tttagatatt gttgatttgt tagatgatga aaaaagaatg ttagaattct taactagcaa
    17461 agttgatttt tcaggcatta atgaaccatg gcttgatatg actaaatcag gaacgtcatt
    17521 aatgtccaaa gctgcaaata caatgcttca atcaaaatta agtgaattct tcactcatgg
    17581 caattttgat ggtgcagtaa atgccaatgg tgtttatcaa tatttagttg atgtggcaca
    17641 gaaagtaatg ggtaagttcc ccggacttac agtaacttct ggatatcgag caggagatgc
    17701 ctactatcac ggaaaacggc aagctatcga cttggcttat ccaggtattt ctggagatcc
    17761 gagatataca gcagcagcaa attacgcttt tgaaaaattc ccttcaaaaa ttgcgtatgt
    17821 cattacgaat ggacgtgtac gtgaccgcat gggattatct ggaacaaggt caagcggaca
    17881 atggacgaat tggccagatg gcgatcactt cgatcatatt catttaaacg gttcgatggg
    17941 gtctggcgat atattcaatg gaggagttgg aggaagtggt gttgcacgct ggcgttctta
    18001 tgtgtctaaa gcattgaaaa tgaatggatt accagctaca gcagcgtata taaatgcctg
    18061 gatgtcccaa atccaaacgg aatctggtgg taatgagaaa gcaatcggcg gcaatgatgg
    18121 gctcgccgaa ggcaatgcaa caggattact tcaaacgaaa cccggaactt ttgcagctaa
    18181 cgcttttcct ggtcacggaa atattatgaa cggatttgat aacatgttag cagcaatcaa
    18241 ttatgctaag aaacgttacg gagttgcagg tatgttgcaa gttatcggga aaggacatgg
    18301 atatgccaat ggcggcttga ttaccaaaga cggactatat cgagcaggag aaggaaataa
    18361 accagaaatg gttattccat tgacaagaaa aacaagagct atcgaactta tgggtcaagc
    18421 attagccttc ctttctggaa gcaataaaaa agaaacgaaa agtgagatta attctgtaga
    18481 taatagacat atcctacaag cgatagaaaa acagtctcgt atgacaaatg atctgctatc
    18541 attattactc tcgtttttta aaagtaacaa tcgatcagat aaagaactag ctttagatat
    18601 tcagaaaatt ttaatcagga ggacatagta tttgaggaca gtaatattaa gaaataaatt
    18661 gaatgaagaa attgatttgt ccacagaaga ttattttgct actgaaatgt cgaatctagg
    18721 ttttgaagtt acaaaagagc atattggaca atgggggagt ttcagagaaa taggagagag
    18781 tattgaattg tctgaatttc aatcatcagt tatgatttct gttcacggat ttagagaaca
    18841 acaattatat ggttcattag ttgaatttct ggctaatgga ccttttgaac tagaattttc
    18901 ttttgattca gaaacaatga tcaggaaatg tagtttgaaa tcattatcta aaacagaaat
    18961 tgattcccaa agctcattgc taacagatac tttgaatcta tattttactt caagctggta
    19021 ttctattaag cgtgaaaaat taatacagaa ttcaacttct ctcaatacac gtggaaaagt
    19081 ttactcattt gttcgaccat atgtctacac caaaaatgta tgggaaaaga aaggtgtatt
    19141 taagttcagt aataattctg tatatttgac aaatagtaca gagcgaatgt ctccgttaaa
    19201 aataaggatt attggagagt gctcaaatcc atattgggaa gttatccaaa actctaaagt
    19261 agtagcttca gatggctatt ttattaatat gaaagacaca caaatattag aagtgtctag
    19321 tttgctagaa gaccaaacgg caattttaaa agatgtaggt ggagttgaat cttctgttta
    19381 tcaacaacaa gattttacta aatcaaactt cgtccaagcg cctctaggag aattcagcgt
    19441 tgtttttcat gttggacatg caaatgtaga gattgaactt tatgaggagc gtgatctatt
    19501 ttgattttag ccattaccct ctttcaccga gatatgaatt tatataacga acgtttaatt
    19561 tcgactgagt tcgaatttgg aacggatgaa attggagaag aagtaagtaa ttttattaca
    19621 gataaatatg tccatgtaaa atcaggagat tttctactag caaaatttat tcctagcggg
    19681 aagattgctt attttggagt gattagctcg caagaagaca aaaaaataaa ttgcaaagga
    19741 ttactcagtt tggccgatag cgagataccg actgtaaaaa tatcaggtgc taattatgaa
    19801 gagcatattc gacgactgat tgaatactac ttattgaacg atccaacaaa acaactaaaa
    19861 aatattttgg aggtaaaaac agaaagcgtt acttctcatt cttatcaagc tgttgacaca
    19921 aataagagac gattgaatgc atatataata aatgggttca aaaaatataa tgttaaatgg
    19981 tatgtcaaag agattaaagg taaaaaaata tatactggta ttcgagcagt tgatgattca
    20041 atttatatta aagataattc atctgagttt aatgactggg atgtttttgt gcaatctcct
    20101 ggaccaggga acgagaacaa attattgatc gtagataaag caatgaaaga tatagaaaat
    20161 cccttggtat tgtctacgtg gtatttagat gacgaaaata atcttacgca agattcaacg
    20221 aataagaaca ttgcattgcc taccgttaat ttagtaaata tatatgatca aactcaagaa
    20281 gatagacctt cttatgaaga ggtagctaaa tcggagctta aaggcaacgc ctattcacat
    20341 gaaattaaag ttaatgttgt gctaaatgct agaaatatca atgtagaaaa tatcgaaaca
    20401 ggaatgttag cgactattgt ttacaaagag cagacttaca aatctgttct aactgcctgg
    20461 cgaatatcca gcagtaaaaa gaatatagaa ttgacatttg gtaatgtccg aagtcgtttt
    20521 atggattatt ttgaagataa tgggggataa aaaatggtta gcaatgtgga tggatatcaa
    20581 tttgaaaacg tgaaagtaag cgcagaaaat gatgctagac tttatcacgt tttatataat
    20641 cggaaaaatc aggttattga tggttacgat cagtctatga atttatcttc aagcggatta
    20701 acagtaaaag ttgctgcagg agcagcgatt attcaaggtc gtatggtcgt tgttcgacaa
    20761 gaagaaagta taacagttcc agcaaactca agtggttata tagcattaac agtggatttg
    20821 acacaagaag ttatacctgg atctattctt ccagaatcgg aagaatatga atggactaat
    20881 aatcaagtca agctagaatt tataacaaaa gttataaaag gtaatttaaa caatggtgat
    20941 aaggtctata atttaccact atgctcagtt aattctactg gatcaactgt ttcaatctca
    21001 aagatatcgg atagttacga gctgactctc tctaaaggag aaattttgtg gagggggact
    21061 gcgttaatgc atgatactca aactgtccaa ccttcaaaaa aaatttggca gacagtttca
    21121 ggttttttgt tagtatggct cccatacgaa aacgggcaag caattgagga tagatatgta
    21181 actacgcctt tttataagga gcgtataact tttactaatg ttttaggaga aattgtttca
    21241 ggatttgatg actatcacaa aaagtggttt agtaaacgaa taaactataa ttcgaatact
    21301 aatatattta caggtgcagc aagcaatgca agtggagata acgcaaatat ggtgcttagg
    21361 tatatagttt ctttttagtt aggaggtatg gaagagtggc aaatttagaa attaaattat
    21421 ctgcaaataa gagacagcct tatctacgtc accgtgttgt tggtagagtt ggtgatgggg
    21481 ggctcacaac aatcaatgta caacttcttg aggaagatga aattacacct tttgtaatta
    21541 atctaaacgg tactttgaaa tttgtgggcg aagtttcaaa cggtaactat accgagggag
    21601 aaccagaaat aatcgattcg actaatgggt taattagtta cacgttcact aagtcaaatt
    21661 tcagcacgag tcatcaattc aaacaagcat attttgaata tgtagatcct aacggcaaaa
    21721 aagtaacttt tcagaacttc atcatagacg tgttagaacg agtagatatt aattcggagc
    21781 aagcgaaata ctatatttct tcattggaaa aattacagag tgaaatgcaa accactttca
    21841 atcagttcat tagtgataaa caggtccaat acgatcaaat ctactcgaaa tataacgaat
    21901 tagtaagatt gataaatgaa tcagataagc aagtaaatga tcgtattgat aaggcaaata
    21961 atcgtattga tcaaaccaat cagcaaatcg gcgatctcgg caagctgaaa aagatgtaca
    22021 gtaacagcat tgatttcggg ggatatgatt atagcggtaa gccgaattta atgtccaaac
    22081 taaaatcgag cgattttaac gttggttacc acgggtcact aacttcggat aacgaaaagc
    22141 tacattttac ttctgatggt acaggaagca ttattatgtt tacgcgtatt aatacacctc
    22201 agcttgctag tgggaaaacc tatactctga gtgcgaaagt tcgatttgat gaaggaacta
    22261 caggagctat tgataaatta cgtttggtgt atcgtacatc accaggagaa aagatattat
    22321 tggaagcaaa tagtacaaat attacaacag atgatgtagg gaaagaaata acaatcaaag
    22381 gtacagctaa cgttaattat caaatcacaa atttagatcg attttatatg agtattagct
    22441 ttgttgacag ggataaaata aatggcggat ttaagttgta cgacatcaaa atcgaagaag
    22501 gctcaacagc cacaccgtac cagcctaact tactagatga cccttactgg gtaggtaaaa
    22561 catcgttggg tgagaatatt gcaaataaag gtacgacgtt cccaattaaa tctagcgcgt
    22621 acgaaatata taaaggtaac acggaagaag agcttataat aggtcaaacg tatactatca
    22681 cgcttaaagg aacaaaaccc gcaagtcaaa cctttgtagc atataattat tggaacgtta
    22741 attttggaga actaaaacca gttgagggat tgacagacgt atggtctcta acattcacac
    22801 caacgaaact tgaaccgggt ttgcctaaag accttcgcat ttttcagttg cctaaagaaa
    22861 cggccggcgc atgccaaatt gactggctca aaattgaaaa aggcgacacc cgaaccccga
    22921 atattagtca gtttaaatac tttggtgaag gattgaaaga tagcaacaat ccgaacgact
    22981 acagctggga catcacgcct gaatatactg aaaaaggctt gaatgatacg gttagtttga
    23041 ctgaaccgca atccatcgaa ggattgaaaa atttcgaaga tggtattcaa tcaaaaggaa
    23101 aatctgtatt gacatcagac gacaacaaat atgaagtcgt aaccttaaca gttacaaacg
    23161 ggaataccgg atcagcaaag ctttatcgtg aaggaaaaac cgtcactatt tatttttttg
    23221 cgttgaatgg gaaaagcagt ggtggaaatg attcaacgat actaacaatt ccagaaggct
    23281 atcggccacc aattagtttt gagcaactgg ttggctcgat agaccgttct actttgaaca
    23341 gtgctcagtt atctattggt gcagatggag ccattaaatg gcgaagaaac tcaagttatg
    23401 gatcagctta ttcatttgtt atcacttatt caatttaagg gaggaaatct aatgaaagta
    23461 gtttacaaat caatcaagcc ttacggattc gagcaaatca ttgtgaatga cgaagaacat
    23521 ttaccggaag aatgcacaga agtcgaacca ccgattccaa attggaaacc gaaattcaat
    23581 tactgggagg gaaataaatg aaaaacattt ggaaatatgg ccgtactggc ggagagtatg
    23641 caggaaaagt attggacgac atgcttgtat ccgttcctta cacagatcag ccaccgctcg
    23701 aaggggttcg ttcagatggc gaaccgctaa cgattgctga tcagatgttt gatcctaaat
    23761 tgaaccaatg gattatttta gcgaacgcgt tagatcacaa cgatttaaac aatctcaaag
    23821 cgatgtacga ggctcttgaa catgaaaacg gcaacctaaa acagctaaat gccaaactca
    23881 tgctaagcga tgtagcgatt aaacaggaaa atactgcatt gaaagaaaaa gctgacagtt
    23941 tagcacaaat caattcaaaa atgatgcttg cttcgttaca aaatagcaaa gacatttcag
    24001 aaattaaaga gcaactaaat ccagcttcaa aaggaggtga gtagtatgtt tagttttagc
    24061 gatgtgaaaa tgatgtatga ttggggctgt tttactgacg atcaagttcg actattcgtt
    24121 ccactatgca ttacagacga agaagcagat aaaatcatta ataaagataa gagcgcatct
    24181 taattgatgc gtttttattt aaggtaaagg agttgtcaca tgattaattt aggggaatgg
    24241 ggaatgatag caggatcaat aaccgctatc gtttctttga ttttattagt aattagaccg
    24301 attgctgcat ctttctcgaa aattactgag actctttcaa aagtaagcca caatttagat
    24361 ttgctgacta aagatttaga atcgagcaaa tcagatcgat tgatgattca tgaagaacta
    24421 aagaaacacg atgaaagatt agatacacat acagaaaaat tggtggaaca cacgcaacaa
    24481 atcaaaactt tgtttaggga gagaagaaaa tgaataataa aacgttcgaa gtactaaaat
    24541 ggttcgcact ggtaattatt cccgcactag ctactttcgt ggggttagtt ggtaaagcgc
    24601 tcaattggca gtacacagat atctgtgttg tcatcattac tggttttggc gcgtttttag
    24661 ggagtgtgtt gggtgtatca aatcgaacct acaaaatgtt ctcggctgaa agcgaagaag
    24721 gaggaaacaa atgaaaaaga aaattactat tactgcgatg agcctgttaa cggctctttt
    24781 tttattgcca attaacggat ttgcctatac tatcaacaat gaatttaatt tgggcgcaaa
    24841 tgaaggtagc tcacaagtag caaataatca gtatatttta ctgcatgaaa cggctaatga
    24901 aacagcaaca ggacgcaatg aagcgcagta tatgcaacgt tcatggacta gcgcttatac
    24961 tgcttatatt gtgggagacg gcggaattgt ttaccaagtc ggacaacctg gttatgtaca
    25021 gtacggtgct ggttcatatg ctaatgccaa tagtcctgtg cagattgagt tacaacacac
    25081 acatgataaa gcaacttttg aaaaaaacta taaagcatac gttgaattgg ctagagattc
    25141 agcaatgaaa tatggtattc cattaacatt agacacgcct tataaccaac caggaatcaa
    25201 atcacattta tgggtaactc aaaacatttg gggcgatcac actgatccgt atggatactt
    25261 atcagaaatg ggtgtaagta aggaaaaact ggcttacgat ttagctcatg gatttacaga
    25321 cgaaaaccca acgacttctg agaacaagcc tgtcattgat ccaacccgag caggtgcagc
    25381 aaatccaact ttatcagatg gaacgaacca ttctcacatt gatcagtttg gagaaatcga
    25441 aaatgcgaac ttacacgtcg ctggatggca catcgctaac tataagtacg agtatatttt
    25501 cattatggac tacaatactg ggaaagaatt agctcgagta agagctgatg gaatttatag
    25561 accggatgta aatcaagctt ataatacttc tggaaatgtt ggctatcatg tatcttttaa
    25621 catgcgtaac ttccctaata agaaagtcta tgtcatgatg cgggcaacga atgatccaga
    25681 gggaaacact aaaggcggtg cgcaagattt ccatgacaaa cgttggtatt taaatattcc
    25741 taaacgataa aaatagctcc tcgttgagga gcattacata actatattga caactataaa
    25801 aatcattcga taaaatagtg atgttatcgc atatcttcac tatcaccca
//
LOCUS       DACEEZ010000031        25787 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20313_2017
            SAMN12639966-rid9879223.denovo.031, whole genome shotgun sequence.
ACCESSION   DACEEZ010000031 DACEEZ010000000
VERSION     DACEEZ010000031.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639966
            Sequence Read Archive: SRR10023158
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 25787)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25787)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741683.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741683.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:44:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,016
            CDSs (total)                      :: 2,962
            Genes (coding)                    :: 2,889
            CDSs (with protein)               :: 2,889
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 36 of 73
            Pseudo Genes (incomplete)         :: 29 of 73
            Pseudo Genes (internal stop)      :: 18 of 73
            Pseudo Genes (multiple problems)  :: 8 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25787
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639966-rid9879223.denovo.031"
                     /isolate="E20313_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            438..992
                     /locus_tag="IWO89_001350"
     CDS             438..992
                     /locus_tag="IWO89_001350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HAQ0456116.1"
                     /translation="MSYNVQPLRTNEEIQDFQFWLRRTSNPERDSFLFLFGINNGLRM
                     SDIIGLKVGDIRGKSKPIIVEHKTGKRKPIFIDNLREEILLYTEGKEENDWLFPSRQQ
                     GRHITRDRVYQIYADIAEKLGRDDIGTHTLRKTFGYHYYKKTRDIATLMFIFNHSSQA
                     ITKRYIGITEDEIGASLRGFKLGV"
     gene            complement(1165..1638)
                     /locus_tag="IWO89_001351"
     CDS             complement(1165..1638)
                     /locus_tag="IWO89_001351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HAQ0456117.1"
                     /translation="MRIKPIKDGEQLLFSSEENKPLYLDYVNHNLKIIIKENNLKHIT
                     LHGFRHTHCSLLFESGASVKEVYVRLGHTDIKTTMDDIYTHIAKKQTEETAIRFADFM
                     SSTEEKQGSLQKVKSMVKNEKSRLSLLDSLLNVELSVFFLFRSVCVFETVLILFF"
     gene            1650..2186
                     /locus_tag="IWO89_001352"
     CDS             1650..2186
                     /locus_tag="IWO89_001352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxalase"
                     /protein_id="HAQ0456118.1"
                     /translation="MPARIHHISIVNRFIRPTFDFYHNILGLKLLMKTINQDDHTMYH
                     LFFSDNHHRVGTELTFFEVQEGNNQFFGTNTIERTILKVPSEASLHFWEIYFETQGVC
                     HYGIESFSGRPILRFEGPDATRLALVPLREFEDIDDYFPYVTDQIPTEHAILGIDAIQ
                     LRVQYSDATERELLSVGW"
     gene            2226..2597
                     /locus_tag="IWO89_001353"
     CDS             2226..2597
                     /locus_tag="IWO89_001353"
                     /inference="COORDINATES: protein motif:HMM:NF013098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxalase"
                     /protein_id="HAQ0456119.1"
                     /translation="MTILENQDTQFYQEVQIIQDRENPVAIEGIGSVHHVAFGVENKS
                     DLQRIDKQLQERNFINSGIKDREFFVSLYYRDPNQLLIEIATGEGNLDAKAYENQSPR
                     FEEIPLFLPQYLNFIREQVEQ"
     gene            2881..4152
                     /locus_tag="IWO89_001354"
     CDS             2881..4152
                     /locus_tag="IWO89_001354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HAQ0456120.1"
                     /translation="MKLSTQEKQLKNVSLKIPKNKISIFTSVSGSGKSSIVFDTITQE
                     AGRQLNDTYDSFTRLFLPKYSRPEVDEIDNLSTAIVVNQKKIGGSARSTLGTITNINA
                     LFRVLFSCFATPSLGYANAYSFNDPSGMCSVCQGIGKTIAFLIGLLYFSAIDNADFVF
                     SDPFTAEVLSGIEEQAKNHGYFVLVHNVNSAEDVKTIQRNWRFEGFIVVGVRVKEFLE
                     LNNVFDGPVVYIDTHLSKETLRELEQQMNRLFVNTNDYQASTLAVEHLIHYGHEKIAF
                     LSFDYIVGEPSVIQERHQAYIDTLKKYHLTFKSHWIYNNSEFERIYQELAEFTAIVVT
                     GDYLAAKLVKFLKNKNKRVMEQLSIIGFDDITFAELMDPALTTIRLNPGNKGAIAFHQ
                     LIGLAKQEEIKNQVLLLEGELISRDSVHKIK"
     gene            4397..5422
                     /locus_tag="IWO89_001355"
     CDS             4397..5422
                     /locus_tag="IWO89_001355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family transcriptional regulator"
                     /protein_id="HAQ0456121.1"
                     /translation="MVGIRDVAKRAGVSIATVSRVLNEDPTLSVTDETKRKIAESVNF
                     YHYVKKSPVAKNLTNIALITTVSEVDELEDPYFRTIRRGIQVEAEQSKVNLKKIIRLS
                     ETPLEQDDLLKCQGVLIIGQVLSSVIEEVSAINSNVVVIDDPSIEAEVDAVYTDLTKA
                     TNQHLDRLYKKGHRNIAFIGGGRVRLDSQGNKHVSDDDYRKIAYETWMKQKGLEKYSQ
                     IYLEGWTTLDGMQAADLLLKKHAKDKLPTAIMVGNDPIAVGVYRSLQKNGLHIPEDIS
                     IVSFDNIEVAEFLTPALNTVNVNTEEIGKLAVRMVNERIKEVRNVSIKVIVSNNIIVR
                     ESEKNID"
     gene            5716..6825
                     /locus_tag="IWO89_001356"
     CDS             5716..6825
                     /locus_tag="IWO89_001356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HAQ0456122.1"
                     /translation="MLDEFEKQNPDIKVEMETTPWDQYWVKLEAATTGGNMPDVVTMH
                     SSESYKYMSQNALMGLDDVIKENDIDMGNFTEGIADFYTLEDELYAIPKDASVVGLWY
                     NKELFDNAGVDYPDETWTWETLRQAAIDLTDESKGIYGFASHIGTETGYWPFIYQNGG
                     EVFADDNTKSGLNTPEAQEALAFYRDIALVDHASPTITELQDPDKLSRFQSGRLAMMI
                     EGNWFAPGFLKNDYLVENVASAVLPKGKVNATITNGLGWAASASTKYPEEVKKLMAFL
                     ASEDANKIQAETGASIPALKGFEDEWAASSDTIDLSAFGKMLEYGVPRPYNKDGLRAE
                     QAEVQIINRILSGEVTVEDGTAQIEKEVNAILSGN"
     gene            6908..7804
                     /locus_tag="IWO89_001357"
                     /pseudo
     CDS             6908..7804
                     /locus_tag="IWO89_001357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008378728.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
     gene            7820..8644
                     /locus_tag="IWO89_001358"
     CDS             7820..8644
                     /locus_tag="IWO89_001358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002579255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HAQ0456123.1"
                     /translation="MKNKFFSNAPIHIMLILGGIAMALPFIWMILTSGKTLVESTQIP
                     PTIFPEKFQLSNYQQVLDLLPFMAFYINTFLMVVGRVIGSVLFSAMAAYACARLSFPG
                     KNILFSLVLIQMMIPGSIFITPQYLLAQKLGILNTIPALILPGVVSAFGTFLLRQFFL
                     GLPDELEEAAYLEGANIWTIFFKIMLPLAKSGLVALGIFTALFAFKDLMWPLIVNTSI
                     DKMPLAPGLANLQGQYATNFPQLMAGSLLAIWPIVVIFIVFQRKFIEGIATTGSKL"
     gene            8676..9986
                     /locus_tag="IWO89_001359"
     CDS             8676..9986
                     /locus_tag="IWO89_001359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005235059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase/alpha-galactosidase"
                     /protein_id="HAQ0456124.1"
                     /translation="MTKITFIGAGSTIFAKNVLGDAMLTPSLQDAHIALYDIDEKRLK
                     ESNLMLKNINKNSNENRADIVSYYDRKEALREADFVINAIQVGGYKPSTVIDFDIPKK
                     YGLRQTIGDTMGIGGIFRTLRTLPVMLDFAKEMEEVCPNAWLLNYTNPMAMLTMGVLK
                     ATSIKTVGLCHSVQVCVPELFEHLGIADQYDLNDFQWKIAGINHMAFLLEITKNGEDF
                     YPTIRQLAAEKPNPHPDSVRFELLKRFGYYITESSEHNAEYHPYFIKSKYPELIDELG
                     IPLDEYLRRCEQQINDWESMREDIVNNGELTHTRSREYASYIMDSITTGTPSVIAGNV
                     LNKGLITNLPTDVCVEVPCLVDKNGVQPTYVGELPTQLAALNRTNINVQELTVEAALT
                     LDKSKIYQAAYLDPHLSSELSLTDIKNLVDDLIEAHGDYLPEYN"
     gene            10134..11393
                     /locus_tag="IWO89_001360"
     CDS             10134..11393
                     /locus_tag="IWO89_001360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HAQ0456125.1"
                     /translation="MKWSKKIILACIALGATISLTACGKNQEDASGKVEIEFFNQKKE
                     MTQTIQEIAKDFEAKNPDIHVKVVDVPNAGEVIKTRMLAGDVPDVINLYPQSIELKEW
                     AKAGYLEDLTEEPYLENIKNGYAQRFAIEDRVYSIPLTANVYGFYYNKTAFEEMGIQA
                     PETWAEFEKIVAEIKDQNKVPFAIAGAEGWTLNGYHQLAIATAAGGEEEANNVWRFSE
                     VNGINAESKEMQKDFNRLDLLREPKALQNNWQGAGYNDTVVTFTKGDALIMPNGSWAM
                     PMIHSQNPDFEVGTFPFPADEAGQSLTIGAGDLALSVSATSQHKEEAKRFVEYMTTPE
                     AMQKYYDVDGAPCAVEGVIENTQDSPLSGLTELAFTDRHLVWLAKDWNSENDFYTLTT
                     NYLHNGNQKAMINALNAFFNPMKADGR"
     gene            11403..12275
                     /locus_tag="IWO89_001361"
     CDS             11403..12275
                     /locus_tag="IWO89_001361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018363968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAQ0456126.1"
                     /translation="MKIKEILNRYWAIIFVAIPIILQMIFFYFPMVQGAFYSLTNWTG
                     LTYNFDFVGLNNYKILFSDPKFIKSIGFTLIVTLCMIAGQIGFGILIARALNANIKGK
                     TFFRAWFFFPAVLSGVVVSLVFKQIFNYGLPKIGSMLGIEFLQTSLLGTTWGAIFAVI
                     FVLLWQGIAMPVIIFLAGFQSIPQEIIEAARMDGANDKQIFWNIEITYLLPSISMVFI
                     MALKGGLTAFDQIFALTGGGPSDATTSLGLLVYNYAFKNNQFGYANAIALVLFILIGI
                     VSLAQLKLSKKFEI"
     gene            12287..13117
                     /locus_tag="IWO89_001362"
     CDS             12287..13117
                     /locus_tag="IWO89_001362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000711263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HAQ0456127.1"
                     /translation="MKTSKEKNISKYLLLLLGGFMILVPLVSTLFGSFKTTPDIMNNF
                     FRFPNPATLENFRRLLADGIGHYFWNSAIITIIAVVAVTAIIPMAAYSLARKMTRGKA
                     YGLMYLFLILGIFVPFQVIMIPITVMMSKIGLANMWGLILLYLTYAVPQTLFLYVGFI
                     KLNVPISLDEAAEIDGASHFTTYFKIVFPMLKPMHATTIILNALWFWNDFMLPLLILN
                     RDSNMWTLPLFQYNYTGQYFNDYGPSFASYTVGIVTITLVYLAFQRSIIAGMSNGAVK
                     "
     gene            13157..15340
                     /locus_tag="IWO89_001363"
     CDS             13157..15340
                     /locus_tag="IWO89_001363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016175926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-galactosidase"
                     /protein_id="HAQ0456128.1"
                     /translation="MIIFDNHTQTFHLQNKKISYLLGIEENNYLTHLYFGKKITHYSG
                     GYRYPRTDRSFSPNNANAQDRLLSLDTLPLEFPGYGHGDFREPAHNIKLGNGSRVNDF
                     RYDSYEIQKGKPSLAGLPALYTEDDDEAETLIIQLKDAISGLTLQLFYTIYQNHSVII
                     RSTQLVNNSDETVEINRLASQSIDFPNRPLELIHLNGTWARERQMTREKIHTGIKVLD
                     SKRGSSSHQQNPFVALVDAKTDEFQGEAYGFNLVYSGNHETVIQKDPFDQTRVIMGIN
                     SFNFGWQLKTGDSFQTPEVVMVYSNEGLNTLSQTYHELYNHRLIRGNYRLKERPTLIN
                     NWEATYFDFNEEKIMNIVDDAKNLGIEMFVLDDGWFGGRENDFTSLGDWVETEGKLAC
                     GLENLAKQVHDNGMKFGIWFEPEMISEDSELFRAHPDWALGIPGRDRSISRNQYVLDF
                     SRQDVRDNIYQQMTAIFDRVPIDYVKWDMNRNMTEVYSVLLDSTMQGEVAHRYMLGLY
                     EFLEKLTTNYPTILFESCSGGGGRFDAGFLYYMPQTWTSDNTDAVARLKIQYGTSLVY
                     PISSMGAHVSAVPNHQTGRETSLTIRGNAAMAGVFGYELDLTQLTSEEKAEIKEQVAF
                     YKKHRQLLQYGTFYRLESPFEGNDTAWMFVSKDQTEAMVFYFRVLAEAAHPLVTLKLA
                     GLAEEATYQMEDVTFAGDELMNLGFYIDSELHGDYATQRFYLKQI"
     gene            15401..16621
                     /locus_tag="IWO89_001364"
     CDS             15401..16621
                     /locus_tag="IWO89_001364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligo-1,6-glucosidase"
                     /protein_id="HAQ0456129.1"
                     /translation="MFYLHAFTKKQPDLNWENEEVREEIYAMINYWLDKGLGGFRIDA
                     ILNIKKKLEYGIFESDGEDGLAFIGPWILNQSGIEVWLKEMRERTFKPHNSMTVAEAD
                     VPNERLDEYIGEDGYYSMVFDFSYTDIDVPETGEWFKDSQWTWTDMRTNIFTNQLVTQ
                     DKGWGALYLENHDQPRSINKYIPEEWINDYSKKMLATLFMLLRGTPFIYQGQELGMTN
                     IEMASLEDFDDVATHSQYKRALEYGLAPEQALKAVNKRSRDNSRTPMQWNTQKNARFS
                     TSDNVWLKVNPNYPELNAEAQLTNQQSVFNYYHQLIKLRKNSVYKDVLIYGQFLPVKD
                     KNEHILLYERQLEDKIVLVILNVTATEQEYYVDEQYQQVLINNYEDVLLDQSRKLRLR
                     PFESIVLANYGGQL"
     gene            16618..18078
                     /gene="gtfA"
                     /locus_tag="IWO89_001365"
     CDS             16618..18078
                     /gene="gtfA"
                     /locus_tag="IWO89_001365"
                     /EC_number="2.4.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sucrose phosphorylase"
                     /protein_id="HAQ0456130.1"
                     /translation="MKIKNEAMLITYADSLGKNMGELKQVLDKHLQGVIGGVHLLPFF
                     PSTGDRGFAPSDYTTVDPALGTWEDVEALGEDYYLMFDFMINHISRESKFFQDFKKNH
                     EQSPYKEMFIRIHEFFPENRPTQEDIDLIYKRKDKAPFQTVEFADGTTEEVWNTFGEE
                     QIDLDVTKEVVKEFIRETIKDMANHGCSLIRLDAFAYAIKKLDTNDFFVEPDIWELLD
                     EVRQEAAKYDVELLPEIHEHYSIQMKIANHDYYIYDFALPMVTLYSLYSGKSERLANW
                     LKMSPMKQFTTLDTHDGIGVVDARDLLTDEELDYTSEELYKVGANVKKVYSSANYNNL
                     DIYQINSTYYSALGDNDRSYLLARAIQVFAPGIPQIYYVGLLAGKNDIELLEATKEGR
                     NINRHYYDLAEIEEEVQRPVIQQLFALLKFRNESAAFDLDGTIEVATPSESEIVITRK
                     NKNGSDSATLTANLDTKEFIIESKDIKIFTSVALKF"
     gene            18155..19261
                     /locus_tag="IWO89_001366"
                     /pseudo
     CDS             18155..19261
                     /locus_tag="IWO89_001366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016175341.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS4 family transposase"
     gene            19583..19792
                     /locus_tag="IWO89_001367"
     CDS             19583..19792
                     /locus_tag="IWO89_001367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3173 domain-containing protein"
                     /protein_id="HAQ0456131.1"
                     /translation="MVVITKNELMKLGYGKYQSERIIRQAKAIMVSKSYTYYENRRLG
                     LVPVESVEEVLGMKIEVEEDFQNHG"
     gene            19785..19934
                     /locus_tag="IWO89_001368"
     CDS             19785..19934
                     /locus_tag="IWO89_001368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrase"
                     /protein_id="HAQ0456132.1"
                     /translation="MVNFKKDKNGKYHFTASLGFDKRTGKRVQKNVEGLKQLKKKKSV
                     YRTCF"
     gene            19969..20753
                     /locus_tag="IWO89_001369"
                     /pseudo
     CDS             19969..20753
                     /locus_tag="IWO89_001369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922412.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
     gene            complement(21037..22122)
                     /gene="xerS"
                     /locus_tag="IWO89_001370"
     CDS             complement(21037..22122)
                     /gene="xerS"
                     /locus_tag="IWO89_001370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerS"
                     /protein_id="HAQ0456133.1"
                     /translation="MKNTEIYQKIDTIILHMPDYIKDFVQDREDKDQSPRTTLEYLKN
                     YKLFYEWLLSESIVPDTSITSIRQLTAYDLNLYKSHLKRRTKENVKKETTKTKLKDNN
                     NLGLSTSTINRNITALKVLFKYLSKSSNNPLGKPYLDNNPMDQVATITDKTTLAARAN
                     AIEKKLFLDKDTQNYLDYIANEYKNTLSKRALIYYQRDVERDLAINALILGSGLRLSE
                     VININLDDLSLDKNNVVVTRKGNKRDAVNIAAFAMEYLANYLAIRKERYKVTENEKAL
                     FLAIYQGEAKRISGIAIERMVAKYSKGFRIQVSPHKLRHTLATRLYQQTNSLVLTAQQ
                     LGHSSTNTTTLYTHIDNAATIDALNAL"
     gene            22456..22689
                     /locus_tag="IWO89_001371"
     CDS             22456..22689
                     /locus_tag="IWO89_001371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="addiction module antitoxin"
                     /protein_id="HAQ0456134.1"
                     /translation="MAIKERKFREVGNSVVLTISKEFLKENGLNPGDTVFLDEEKLRA
                     AITKKQALSDEIDLAISQALTEYDTTFRELVDK"
     gene            22686..23093
                     /locus_tag="IWO89_001372"
     CDS             22686..23093
                     /locus_tag="IWO89_001372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system death-on-curing
                     family toxin"
                     /protein_id="HAQ0456135.1"
                     /translation="MSIHYLEAKDIIKMNVIQIKKYSPNEPLGVKDANALEMCVGQLR
                     ASAFGEEVYPSIYEKAAILLIQLIKKHPFHNANKRTAFLATFVFLKINGQLLAIDQKD
                     AVNLAVYIATYDKDFDQLKYEVINTIKTHTQPI"
     gene            complement(24230..24748)
                     /locus_tag="IWO89_001373"
     CDS             complement(24230..24748)
                     /locus_tag="IWO89_001373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013852041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ClbS/DfsB family four-helix bundle protein"
                     /protein_id="HAQ0456136.1"
                     /translation="MRTYKNKEELKKEINRTYTKYILEFETIPEELKDKRVEEVDRTP
                     AENLAYQVGWTNLILKWENDERNGLSVKTPSDQFKWNQLGELYKWFTNTYAHLPLKEL
                     EEILNRNIDDINMMIDSMSDEDLFTAHKRKWADEATKTAVWEVYKFIHVNTVAPFGTF
                     RTKIRKWKRLAL"
BASE COUNT         8786 a         3930 c         4795 g         8276 t
ORIGIN      
        1 aattattgac gctcccgaca aaattatgat cttgcgtaaa gttataaaat attaataaaa
       61 atattttatt aatgaaaagc ttggtagcac ggttattcat atgctaattt atttcacttt
      121 atagtaaggg gagttcttag tatattgttc aaacttaaat ttcatataat ttaattatgt
      181 aaattagcta ttctaagaac ttctacggat aactgcccgt ctaaaattta aataagagct
      241 tagaatcttc atataagtaa ggttaatttt tttgtacaaa aaagtcggaa tattctgagt
      301 tactgaaatg atataaaatc ataaactaaa tcccttgatt ttattgactt tcaaaagttt
      361 atatatataa gttaatgatt atggtaaaat atatacataa tcattaactc agaatatttt
      421 taagaaggga aacaatagtg agctataatg tacaaccgtt aaggacaaat gaagagatac
      481 aagattttca gttttggcta agaagaacaa gtaatccgga aagagatagt tttttatttt
      541 tgtttggaat caataatggg ttacgtatgt cagatattat aggattaaaa gttggagata
      601 tacgaggcaa aagtaagcct attattgttg aacataaaac aggtaagcga aaaccgattt
      661 ttatagataa tttgagagaa gaaattttat tatatactga aggtaaagaa gaaaatgatt
      721 ggttatttcc gagtcggcaa caaggtcgac atataacaag ggatcgtgtc tatcagatat
      781 atgcagatat agcagaaaaa ttaggacgag atgatatagg aacacataca ttaagaaaaa
      841 cttttgggta tcactactac aagaaaacta gagacattgc taccttaatg tttattttta
      901 atcatagctc gcaagcaatt acaaaaaggt acattggtat aactgaagat gaaataggag
      961 catcacttcg tggatttaaa ctaggtgtat aatttaaaaa tttttataaa cagtaaagac
     1021 aaagattaaa agctattttt atctagaacg attttatttc ataaagttcg cggaataaaa
     1081 gctggggtta ctggtacttg gtataggttc gtgttatata aactaagtaa ttaaataaca
     1141 tttttaggtt ggaaaaggat gaaattaaaa aaagagaatc aatacggtct caaaaacgca
     1201 aacgctgcgg aataagaaaa atactgataa ctcaacgttt agcagactat ctaataggga
     1261 tagtctgctt ttttcgtttt tgaccatact tttgaccttc tgcaaacttc cttgcttttc
     1321 ctctgtagaa ctcatgaaat ctgcaaaacg aattgccgtt tcctctgtct gctttttcgc
     1381 gatatgggtg tatatatcat ccatagtggt tttaatatct gtatgtccaa gtcgaacata
     1441 cacttctttg acagacgcac cagattcaaa gagcagacta caatgggtat gacgaaaacc
     1501 atgaagtgtg atatgtttta agttattctc tttaatgatg attttcaaat tgtgatttac
     1561 atagtctaaa taaaggggct tattttcttc tgatgaaaac aaaagttgtt caccatcttt
     1621 tataggtttg attctcattt ttagatatta tgcccgcacg tattcatcac atttctattg
     1681 tgaatcgctt tattcgaccg acatttgact tttatcataa tatactcggt ttaaaattac
     1741 taatgaaaac aattaatcaa gacgatcata ccatgtatca cctgtttttt tcagataatc
     1801 accaccgtgt gggaactgaa ctgacttttt ttgaagtaca agaagggaac aaccagtttt
     1861 ttggaaccaa tacgattgaa cgaacgatac taaaagttcc ttccgaagca tcattgcatt
     1921 tttgggaaat atattttgaa acacaaggcg tttgccatta tggaatcgaa tcattttctg
     1981 gacgacctat cttacgtttt gaagggccag atgcaacacg attagcttta gttcccttac
     2041 gcgaatttga agatattgat gattatttcc cgtatgtcac agaccagatc cctacagagc
     2101 atgccatttt aggaattgat gccattcaac tacgcgtaca atattccgat gcgactgaac
     2161 gagagttatt gtcggtaggc tggtagcaca aagaatacgt cagtttcttt tcgggtcatc
     2221 aacgagtgac aatcttagaa aatcaagaca ctcagttcta tcaagaagtt cagattattc
     2281 aagatcgtga gaatccagtt gccattgaag ggattggtag cgtccatcat gttgcgtttg
     2341 gcgtagaaaa taaatctgat ttacaacgca tcgacaaaca attacaagag cgcaatttta
     2401 taaattcagg aataaaagat cgtgaatttt ttgtatcact ttattatcgt gacccaaatc
     2461 aattattgat tgaaattgct acaggggaag gaaacctaga tgcaaaagcg tatgaaaatc
     2521 aaagtccacg ctttgaagaa attcctttat ttttaccgca gtacttaaat ttcattcgtg
     2581 aacaagtgga gcaataatta gctaatcaaa tccatgacga agagtaagag gttgactcat
     2641 aagtcaaaaa ataaagaaaa acgcatcaaa tccattctaa agaatgttga tttgatgcgg
     2701 ttcctatttt tataaactat cattttcgac taataaaact acttatgagt cagcctctct
     2761 ttttttcttg atttttatac ctctttctag gtaaactaaa catctgccaa aaataccgtg
     2821 tctcattgaa acattctcaa ctatttcaaa ataaagggga gtaccaaatg aacgagtata
     2881 ttgaaattat ccacgcaaga gaaacaactt aaaaatgtca gtctcaaaat tcccaaaaat
     2941 aaaattagta tttttaccag cgtatctggt tcaggaaaat catcgattgt ttttgatact
     3001 attacccaag aagctggtcg tcaactgaat gacacctatg atagttttac ccgtttattt
     3061 ttacctaaat acagtcgtcc agaggttgat gagattgata atctttctac ggcgattgtg
     3121 gtcaatcaaa agaagattgg tggcagtgcg cgctcaacgt tagggacgat tactaacatc
     3181 aatgcgttat ttcgtgtgtt gttttcatgt tttgcaacac ctagtttagg atatgcaaat
     3241 gcttattcat ttaatgatcc tagtggaatg tgttctgtct gtcagggaat cggtaagacg
     3301 attgcatttt taataggatt gctttatttt tcggctattg ataatgctga ttttgtattt
     3361 tctgatccat ttaccgcaga agttttgtct ggaattgagg aacaggcaaa gaatcatggt
     3421 tattttgtat tggtgcataa tgttaattca gcagaagatg ttaaaactat tcagagaaat
     3481 tggcgttttg aggggttcat tgttgttggt gtacgtgtaa aagaattttt ggaattaaat
     3541 aacgtttttg atggtcctgt tgtttatatt gatacgcatc tttcaaaaga gacattaaga
     3601 gaacttgaac aacaaatgaa tcgtttattt gtaaatacaa atgattacca agctagtaca
     3661 ttggcagtgg aacatttgat tcattatggt catgagaaaa tagcgtttct ttcttttgac
     3721 tacattgtag gtgaaccaag tgttattcaa gaacgacatc aagcttatat cgatacatta
     3781 aagaaatatc atttaacctt taaatcgcat tggatatata acaactctga atttgagcga
     3841 atttatcaag aattagctga atttactgca attgttgtta ctggagatta tttagcggct
     3901 aaattagtaa aatttttaaa aaataagaat aaacgagtga tggaacaatt atcgattatt
     3961 ggatttgatg atattacttt tgcagaatta atggatcctg cgttgacaac aatacgacta
     4021 aatccaggta acaagggggc gattgctttt caccaactga ttggactggc caaacaagaa
     4081 gaaataaaaa atcaagtact actattagag ggagaattga tttctcgaga ctctgttcac
     4141 aaaatcaaat aaaaaagctc tgtgaaataa gtatagttaa caataaaata tccgaacgat
     4201 agagagaatg aactgtgatc tagcagttcc tcgtcgcaaa ttcactagaa ttagcattat
     4261 ttactctaaa ttgttcggat attttttctt gtttttaatt atgattacag tgcctatttt
     4321 taacgaaatt tgcttatttt caaaatgtat gattatatgt tagtaaacac tttcaaatat
     4381 gaaaagagga gctattatgg taggaattag ggatgttgca aaacgggcgg gtgtgtccat
     4441 tgcaacagta tcgcgcgtgc taaatgagga tccgacactt tcagtaaccg atgaaaccaa
     4501 acgaaagatt gcagaatcgg ttaattttta tcattatgtc aaaaaaagtc ccgttgcaaa
     4561 aaatttaaca aatattgcat taataacgac agtttctgaa gtagatgagt tggaagatcc
     4621 ctattttcga acgattcgca gagggattca agtcgaagca gaacaaagca aagtcaattt
     4681 gaagaaaatt attcgtttgt cagaaacacc gttggagcaa gacgatttgc taaaatgtca
     4741 aggtgtgttg attattgggc aggtgctatc ttctgtgatt gaagaagtat cagcaattaa
     4801 ttcgaatgtc gtagtgattg atgaccctag tattgaagct gaagtagatg cagtttatac
     4861 tgatttaaca aaagcaacga atcagcattt agatcgattg tataaaaaag gacaccgaaa
     4921 cattgcgttt atcggaggag gacgtgttcg tttagatagc caaggaaata aacacgtgtc
     4981 agatgatgat tatcgaaaaa tagcctatga gacgtggatg aaacaaaaag gattagaaaa
     5041 atatagtcaa atttatttag aaggctggac cacacttgac ggaatgcagg cagcagattt
     5101 acttttgaaa aaacatgcta aagataagtt gccgactgct atcatggtag gaaatgatcc
     5161 tattgctgtg ggggtttatc gttcacttca aaaaaatggt ctacatattc cagaagatat
     5221 ttcgattgtg agttttgata atattgaagt tgctgaattt ttaacacctg cattaaatac
     5281 ggtgaatgtg aatacagaag aaattggaaa actagcagtt cgcatggtta atgaacgaat
     5341 aaaagaagtt agaaatgttt cgatcaaagt gattgtttct aataatatta ttgttagaga
     5401 aagtgaaaaa aatatcgatt aaaaaagtaa acatccaaaa aagggtgttt acttttttgt
     5461 ttactaatat ttttagtaaa aaatctcgac aaaacattaa gtaaacagtt atggtgttgt
     5521 taacaaaaag aaaacgcatt caaaacagga ggaattaaga tgaaacattt caaaaaaata
     5581 gctttaacag ggatagctgt tttaactggg acattactcg cagcatgtgg tgggggagat
     5641 agtaattcta aagacgggca agtaacttta agctatgcga gttgagatac tgaccaagct
     5701 ccaggactaa gaaaaatgtt agatgaattt gaaaaacaaa atcctgatat caaagtagaa
     5761 atggagacaa cgccttggga tcaatattgg gttaaattag aagctgcaac aacgggaggt
     5821 aatatgccag atgtagtaac catgcactct agcgaatcct ataaatatat gtcacagaat
     5881 gctttgatgg gacttgatga tgttatcaaa gaaaatgata ttgacatggg aaactttact
     5941 gaaggaattg ctgatttcta tacgcttgaa gacgaactat acgcaatccc taaagatgct
     6001 tctgtagtgg ggctatggta caacaaagaa ttatttgata atgcaggtgt cgattatcca
     6061 gatgaaacat ggacatggga aacactcaga caagctgcaa ttgatttaac agatgaatca
     6121 aaaggcatct atggttttgc atcacatatt ggaacagaga ctggctactg gccatttatt
     6181 tatcaaaatg ggggagaagt atttgccgat gacaatacaa aatcaggttt gaatacccca
     6241 gaagcacaag aagctttagc tttttatcga gatattgcac ttgttgatca tgcttcacca
     6301 acaattacag aattacaaga tcctgataag ttaagtcgtt ttcaatcagg tagattagca
     6361 atgatgattg aaggaaactg gtttgcaccg ggatttttga aaaatgacta tctagttgaa
     6421 aatgttgctt ctgccgtgtt acctaaggga aaagttaatg caacaattac aaatggactt
     6481 ggttgggctg ccagtgcgag tacaaaatac ccagaagaag tgaaaaaact aatggcattt
     6541 ttagctagtg aggacgccaa taaaatccaa gcggaaacgg gggcatcaat cccagcacta
     6601 aaaggctttg aagacgagtg ggctgcttct tcagatacaa ttgatttgag tgcttttgga
     6661 aaaatgttgg agtatggagt ccctcgtcca tacaataaag atggattacg agctgaacaa
     6721 gcagaagttc aaattattaa ccgtattctg tctggtgaag taactgttga agatggaacc
     6781 gctcaaattg aaaaagaagt caatgcgata ttgagcggaa attaaattag ttaattatac
     6841 aatatacagg cgaaaggatt ctatttcgtc tgtatattaa tggatgaata ccaaaaaggg
     6901 ggatattatg aaaaatcgaa tttttggaaa atatcgtaaa gatcaagtga tatggggcta
     6961 tatttttatt tttccaacgg cattaggatt aattttatta aatatatggc cagcaattca
     7021 aacagcaatt ttaagttttc aaaaaacggt agggttcggt gatactgaat gggtcggatt
     7081 aagaaattac caaaaaatgt ttgcagacgg tgaagttttt cagtctttat taaacacatt
     7141 aatttatgca gtggtttcga tacctagtat tgttgtatta tccttgttgg tggcagtttt
     7201 aatgaataga aaagttaaag gattgtcgat ttatcggaca atttattttc taccagtcgt
     7261 tgctgcacca tccgcagtcg caatgatttg gcgctggatg ttcaataatg attatggact
     7321 gattaataac atgttggcta aaataggtct cgatggacca gcatggttaa cggatccatc
     7381 gattgcgatt tattctatta tcattgtagg agtttggagt gctattggtt ataacatggt
     7441 gttattacta gccggcttat aagaaattcc taaagattat tatgaagcag cggaaattga
     7501 tggtgctagt ccaatccagc agtttttcaa cattacgtta ccattagtta caccaacatt
     7561 attttttgta gttgtaacaa cagttatcaa tgctttccaa gtatttgatg ttatttttat
     7621 gatgattact cctaatagta ccgccatgtt taaatcacaa tcattagcct atttattcta
     7681 taaacattca tttattttaa atgacaaggg atatggttcg gcaatcgtta tgttcctttt
     7741 agtgattata ttaattatta ctgcaattca aatgaagttg cagaaaaagt gggtaaacta
     7801 ttaaggaggc tatcaaatta tgaaaaataa atttttttca aatgcgccaa tccatatcat
     7861 gcttatttta ggtggtattg caatggcatt accttttatt tggatgattt taacttcagg
     7921 gaaaacgttg gttgaatcta cccaaattcc acctacaatt ttccctgaga aatttcagct
     7981 aagtaactat caacaagtac ttgatttatt accattcatg gctttttata tcaatacatt
     8041 tttaatggtt gttggacgag tgattggctc ggttttattt tcagcaatgg ctgcatatgc
     8101 ttgtgcgcgt ctaagttttc cagggaaaaa tatccttttc agcttagtac ttattcaaat
     8161 gatgattccc ggttccattt ttattactcc acagtattta ttagcacaaa aattgggtat
     8221 tttaaataca attccagcat taatattacc aggggtagtt agtgcgtttg gtacgttttt
     8281 attaagacaa tttttcttag gtttacctga tgaattggag gaagcggcct atttagaagg
     8341 tgccaatatt tggacgatct ttttcaaaat tatgttgccg cttgctaaat caggattggt
     8401 tgcacttgga atattcacag cgttgtttgc atttaaagat ttaatgtggc cattgattgt
     8461 caatacttca attgataaga tgcctttagc tccgggatta gcaaacttac aaggacaata
     8521 tgccacgaac ttcccacaat taatggcagg ttcgttatta gcaatttggc caatagttgt
     8581 gatattcatc gttttccaaa gaaaattcat tgaaggaatt gctacaacag gtagtaaatt
     8641 ataaaactat taattagtga ttggagagta agtacatgac aaaaattaca tttattggtg
     8701 caggaagtac tatttttgca aaaaacgtat taggcgatgc gatgttaact ccaagtctac
     8761 aagatgcaca tattgcattg tacgatattg atgaaaaacg actaaaagaa tcaaatttaa
     8821 tgcttaaaaa tattaataaa aattcgaatg aaaatcgtgc tgatattgtt tcttattatg
     8881 atcgcaaaga agcgttaaga gaggctgatt ttgttatcaa tgctattcaa gtgggtggtt
     8941 ataaaccttc aacagtgatt gattttgata ttcctaaaaa atatgggttg cgacaaacta
     9001 ttggtgacac catggggatt ggtgggattt ttagaaccct acggacacta ccagtaatgt
     9061 tagactttgc taaagaaatg gaagaagttt gtccaaatgc ttggttattg aactatacaa
     9121 atccgatggc gatgttaacc atgggtgttt taaaagcgac gagtattaag actgttggtt
     9181 tatgtcatag cgttcaagta tgcgtaccag aattgtttga gcatttagga atagccgatc
     9241 aatatgactt aaatgacttt caatggaaaa ttgcaggcat taatcatatg gcattcttgt
     9301 tggaaattac aaaaaatggg gaagatttct atccaacaat tcgtcagtta gctgcagaaa
     9361 aaccaaatcc acatccagat tctgtacgtt ttgaattgtt gaaacgtttt ggttactata
     9421 ttacagaatc aagtgaacac aatgcagaat accatccata ctttattaaa tcaaaatacc
     9481 cagaattgat tgacgaatta gggattccgt tggatgaata cttacgtcgt tgtgaacaac
     9541 aaattaatga ttgggaaagc atgcgagagg acattgtaaa taatggggag ttaacacata
     9601 cgagaagccg tgaatatgct tcatatatca tggattctat tacgacagga actccgtcag
     9661 tgattgcagg aaatgtctta aataaaggct taatcacaaa cttaccgaca gatgtttgtg
     9721 tggaagtacc ttgtttagtc gataaaaatg gcgtgcaacc aacctatgtc ggtgaattgc
     9781 caacacaatt agctgcttta aatcggacga atatcaatgt tcaagaactg acagtagaag
     9841 ccgctttaac actagataaa tcaaaaattt atcaagcagc gtatttagat ccacatctat
     9901 catctgaatt gtctttaaca gatatcaaaa atttagtgga tgatttaatc gaagcacatg
     9961 gggactattt acctgaatat aattaaaaag aagctgaaag agtaaggaat tttcaattcc
    10021 ttactctttt cttagttaaa tttagtaaaa atattgacaa aacacttagt aaacagttac
    10081 attagtgtta acaaaaagaa aacgcattca aattgtataa ggaggagcat aaaatgaagt
    10141 ggtctaaaaa aattatttta gcttgtatag cactaggagc aacgatctct ttgactgctt
    10201 gtggaaaaaa tcaggaagat gcaagcggta aggtagaaat agagtttttt aatcaaaaaa
    10261 aagaaatgac tcaaacgatt caggagattg ctaaagattt tgaagcaaaa aaccctgata
    10321 ttcatgtaaa agttgtggat gtaccgaatg ctggtgaagt aattaaaaca cgtatgcttg
    10381 caggcgatgt accagatgtc attaatcttt atcctcaaag tatagaattg aaagaatggg
    10441 cgaaagctgg ttatttagag gacttaaccg aagaaccgta tttagagaat atcaaaaatg
    10501 gatatgcaca acgttttgct attgaggatc gtgtatatag cataccatta accgctaatg
    10561 tctatggttt ttactataac aaaactgctt ttgaggaaat ggggattcaa gcaccagaaa
    10621 cttgggcaga atttgaaaaa atagtagctg agattaaaga tcaaaacaaa gtaccgtttg
    10681 cgattgcagg agcagaaggc tggactttaa atggttatca ccaactagct atagcgactg
    10741 ctgctggtgg tgaagaggaa gcgaacaatg tttggcgttt ctcagaagtt aatggaatta
    10801 atgctgaaag caaagaaatg caaaaagatt ttaatcgatt agatttgtta agagaaccaa
    10861 aagctttgca aaacaactgg caaggtgccg gttataacga tacagtagtt acttttacaa
    10921 aaggtgatgc gttgattatg ccgaatggtt catgggcaat gccgatgatt cattcacaaa
    10981 atccagattt tgaagtagga acttttcctt ttccagcaga tgaagcaggt caaagtctaa
    11041 cgattggcgc aggagaccta gccttatcag tttctgcaac ttctcaacat aaagaagaag
    11101 ccaaacgttt tgtagaatat atgacaacac ctgaagcgat gcagaaatat tatgatgtag
    11161 atggtgcacc gtgtgctgtt gagggcgtta tcgagaatac tcaggattca ccattaagtg
    11221 gtttaactga acttgctttt actgatcgtc atttagtttg gttagccaaa gattggaata
    11281 gtgaaaatga tttttatacc ttaacaacaa attatttaca caatggcaat caaaaagcca
    11341 tgattaacgc attaaatgct ttctttaatc caatgaaagc tgacggaagg taggaaaata
    11401 aaatgaaaat aaaagaaatt ttaaatcgtt actgggcaat catttttgta gcaattccaa
    11461 ttattttgca gatgattttt ttctactttc caatggttca aggtgctttt tacagtttaa
    11521 ctaattggac tggtctgact tataattttg attttgtggg tttgaacaat tataagattc
    11581 ttttttctga tccgaagttt atcaaatcga ttggcttcac tttaattgtg acgctttgca
    11641 tgattgctgg acaaattggt tttggtattt tgattgcgcg ggcgttgaac gcaaatatta
    11701 aagggaaaac attctttaga gcatggttct tttttccagc ggtcttatcg ggcgtggttg
    11761 tttctttagt atttaaacaa atttttaact atggtttacc aaaaattggt agtatgttag
    11821 gcattgaatt tttacaaacc agtcttttag gaacaacatg gggagcgatt tttgcagtaa
    11881 tttttgtttt actttggcaa ggaattgcca tgccagtcat cattttctta gcgggttttc
    11941 aaagtattcc acaagaaatt attgaagctg ctagaatgga tggggcgaat gataagcaaa
    12001 ttttttggaa tattgaaatc acctacttat taccaagtat ttcaatggta tttattatgg
    12061 cattaaaagg tggcttaaca gcatttgatc aaatttttgc tttaactggt ggtggaccaa
    12121 gcgatgccac aacatctttg gggttacttg tttataatta tgcttttaaa aacaatcagt
    12181 ttggttatgc gaatgctatt gctttagtct tatttatctt aattgggatt gtttcactgg
    12241 cacaattaaa attatcgaaa aaatttgaaa tctaggaggt ttcccaatga aaacatcaaa
    12301 agaaaaaaat atttctaaat acttgctttt attattaggg ggcttcatga ttttagttcc
    12361 cttagtttca acgctattcg gttcatttaa aacaacaccc gatattatga ataatttctt
    12421 tcgttttcct aatccagcga ctttggaaaa tttccgaaga ttgttagcgg atggaattgg
    12481 tcattacttt tggaattcgg caatcatcac aatcattgca gtagttgctg tgacagcaat
    12541 tattcctatg gctgcttatt cactagcaag aaaaatgact cgtgggaaag catacggatt
    12601 gatgtattta tttttaattt taggaatttt tgttcctttc caagtaatta tgattccgat
    12661 taccgttatg atgagtaaaa ttggtttagc aaatatgtgg ggattaatct tactctatct
    12721 cacttatgcc gtaccacaga cactattttt atatgttggt tttattaaac taaatgtgcc
    12781 tatcagtctg gatgaggctg ctgaaattga tggggctagt cactttacaa cttatttcaa
    12841 aattgttttc ccaatgttaa aaccaatgca tgcgacgact attattctta atgctctatg
    12901 gttttggaat gatttcatgc taccattatt aattttaaat cgtgattcta atatgtggac
    12961 actaccattg tttcaatata attatacggg acaatatttt aacgactatg gtccaagttt
    13021 tgcttcctat acagtaggaa ttgtcacgat tacactagtt tatcttgctt tccaaagaag
    13081 tattatagct ggaatgagca atggagcagt gaaataacca ataaattatt ctcattataa
    13141 aggagttcat aaacttatga ttatatttga caatcacaca caaacatttc atttacaaaa
    13201 taaaaaaatt agctatctat taggaatcga agaaaataat tatttaacgc atctttattt
    13261 tggaaaaaag attacgcact attctggtgg gtatcgctac ccccgaacag atcgttcctt
    13321 ctcacccaac aatgcaaatg cgcaagatcg tttattatct ttagacacat taccattaga
    13381 atttccagga tatggacatg gtgattttag agaaccggcg cataatatta aattaggaaa
    13441 cggtagtcgt gtaaatgatt ttcgttatga tagctacgaa attcaaaaag gaaagcccag
    13501 cttagcggga ttgccagcct tatatacgga agatgatgat gaagctgaaa cgctgattat
    13561 tcaattgaaa gacgcgatta gtggattgac tttacagttg ttctacacaa tttatcaaaa
    13621 tcacagtgta attattcgtt ctacgcaatt agtaaataat agtgatgaaa cagtggagat
    13681 taaccgctta gctagtcaat caattgattt tccaaatcgt ccactcgagt taattcattt
    13741 aaatggtacc tgggcaagag aacgtcaaat gactagagaa aaaattcata cagggataaa
    13801 agtattagat agtaaacgcg gatcaagcag ccatcaacaa aatccgtttg ttgctttagt
    13861 agatgccaaa acagatgaat ttcaaggaga ggcatatgga tttaatttag tttatagcgg
    13921 taatcacgaa acggtgattc aaaaagatcc atttgaccaa acacgggtca ttatgggaat
    13981 taattcattt aattttggtt ggcaactaaa aactggcgac tcatttcaaa cgccagaagt
    14041 cgtgatggtt tatagtaatg aaggtctgaa tacgttatca cagacgtatc atgaattgta
    14101 taatcaccgt ttaattcgtg gaaactatcg tttaaaagaa cgtcccacgt taatcaacaa
    14161 ctgggaagca acgtattttg attttaacga agaaaaaatc atgaatattg tcgatgatgc
    14221 gaagaacttg ggcattgaaa tgtttgtttt agatgatggt tggtttggcg gtcgtgaaaa
    14281 tgacttcact tcattaggtg attgggttga aacagaaggc aaacttgctt gtggtttgga
    14341 aaatttagcc aagcaagtgc atgataacgg gatgaaattt gggatttggt ttgaaccgga
    14401 aatgatttct gaagatagtg aactatttcg agcacatcca gattgggcat tgggtattcc
    14461 aggtcgtgac cgctcaatta gtcgaaatca atatgttctt gatttttcaa gacaagatgt
    14521 tcgtgataat atctatcaac aaatgacggc gatttttgat cgtgtaccga ttgattatgt
    14581 gaagtgggat atgaatcgaa atatgacaga agtctattct gtcttgttgg actcaacgat
    14641 gcaaggtgaa gtggctcatc gttatatgtt agggttatat gaatttttag aaaagttaac
    14701 aacgaattat ccaactattt tatttgaaag ttgttcgggt ggaggcggtc gttttgatgc
    14761 aggtttcttg tattacatgc cacaaacttg gacgagcgac aataccgatg ccgtggctcg
    14821 tctgaaaatt caatatggaa ccagcttagt gtatccaatt tcaagtatgg gtgcgcatgt
    14881 ttcagcagtc ccaaatcatc aaacaggaag agaaacgagt ttaactattc gtggcaatgc
    14941 tgcgatggct ggtgtttttg gttatgagct agatctaacg caattaactt ctgaagaaaa
    15001 agcagaaatt aaagaacaag ttgcctttta taaaaagcat cgccaacttt tgcaatatgg
    15061 aactttttat cgcttggaaa gtccgtttga aggcaatgat actgcatgga tgtttgtctc
    15121 aaaagaccaa acagaagcca tggtctttta tttccgagta ttagcagaag ctgcgcatcc
    15181 attagtcaca ttgaaattag caggattagc agaagaagca acttatcaaa tggaagatgt
    15241 aacatttgct ggcgatgaat tgatgaatct cggtttttat attgactcag aactacatgg
    15301 tgattatgcg acacaacgct tttatttgaa acagatatag tagcttgaat ttacaatata
    15361 agtgcaacac caaaagagac tcattccaaa cgaagaaaat atgttttatt tacacgcatt
    15421 tactaaaaag cagcctgatt tgaattggga aaatgaagaa gtacgtgaag aaatctatgc
    15481 aatgattaac tattggttag ataaaggact aggcggattt agaatcgatg cgatattaaa
    15541 catcaaaaaa aaactagagt atggcatctt tgaatctgat ggagaagatg ggttagcctt
    15601 cattgggccc tggattctta atcagtcggg cattgaagtg tggttaaaag aaatgagaga
    15661 gcggacattt aaaccacata atagtatgac ggtagcagaa gccgatgttc ctaatgaacg
    15721 tttagatgaa tatattggtg aagatggcta ttattcgatg gtttttgatt ttagttatac
    15781 ggatattgat gtacctgaaa ctggtgaatg gtttaaagat agccaatgga catggacgga
    15841 tatgcgtacc aatattttta cgaatcaatt agttacgcaa gataaaggtt ggggcgcctt
    15901 atatttagaa aaccacgatc aaccacgttc aattaataaa tatattccag aagaatggat
    15961 caatgattat agtaaaaaaa tgttggcgac gctttttatg ttactaagag gtaccccttt
    16021 tatttatcaa ggacaagaac taggaatgac caacatagaa atggcatcat tagaagattt
    16081 tgatgatgta gctacgcatt ctcagtataa acgggcatta gaatatggat tggcaccaga
    16141 gcaagcattg aaagctgtca ataaacgaag tcgggataat tctcgcacac ctatgcaatg
    16201 gaatacgcaa aaaaatgcca gattctcaac ttctgataat gtatggttaa aagtaaatcc
    16261 taattatcca gaactcaatg cagaagctca gttaaccaat caacagtcgg tattcaatta
    16321 ttatcatcaa ttaattaaat taagaaaaaa tagtgtatac aaagacgtgc taatctacgg
    16381 acaatttcta ccagtgaaag ataaaaatga acatattttg ctttatgaac gccaattaga
    16441 agataaaatc gtattagtta tcttaaacgt tacagcaaca gaacaagaat attatgtaga
    16501 tgaacagtac caacaagtat taatcaataa ttatgaagat gttttattag atcaaagtcg
    16561 aaaattgcgt ttacgaccgt ttgaaagtat tgtattagct aattatggag gacaattatg
    16621 aaaattaaaa acgaagcaat gctcatcacc tatgcagata gtttaggaaa aaacatgggc
    16681 gaattaaaac aagtcttaga caaacattta caaggtgtta ttggtggtgt acatctgttg
    16741 cctttttttc catcgacagg tgatcgaggc tttgcgccga gtgattatac gacagttgat
    16801 ccagctcttg gaacatggga agatgtggaa gcattaggtg aagattatta tttgatgttt
    16861 gattttatga ttaatcacat ttctcgtgaa tcaaaatttt tccaagattt caaaaaaaac
    16921 catgagcagt caccatacaa agaaatgttt atccgtattc atgaattttt cccagaaaat
    16981 cgaccaacac aagaagatat tgatttaatc tacaagcgta aagacaaagc gccgtttcaa
    17041 acggtagaat ttgcggatgg tacgacagaa gaggtttgga atacctttgg ggaagaacaa
    17101 attgatttag atgtgaccaa agaagttgtg aaagaattta ttcgagaaac catcaaagat
    17161 atggcgaacc atggctgttc tttaattcga ttagatgcgt ttgcttatgc gattaaaaaa
    17221 ttagatacca atgatttctt cgtggaacca gatatttggg aacttttaga cgaagtgcgt
    17281 caagaagcag cgaaatatga cgtcgaatta ttaccagaaa tccatgagca ttattccatt
    17341 caaatgaaaa ttgccaatca tgattattat atctatgact tcgccttgcc aatggtcact
    17401 ctgtattcac tttatagtgg aaaatcagaa cgtttagcca attggttgaa aatgagtccg
    17461 atgaagcagt ttacaacatt ggatacgcat gatggaattg gtgttgtgga cgctcgtgat
    17521 ttattaacag atgaagaatt ggattacacc tctgaggaat tgtataaagt cggggcgaat
    17581 gtgaagaagg tctattcaag tgccaattac aataatttag atatttatca aattaacagc
    17641 acctattata gtgcattagg cgataatgac cgtagctatc tgttagcacg tgcgattcaa
    17701 gtatttgcgc cagggattcc acaaatttat tacgttgggt tattagcagg aaaaaatgat
    17761 attgaactgt tggaagcaac gaaagaaggg cgtaatatta atcgtcatta ctatgattta
    17821 gccgaaattg aagaagaagt gcaacggcca gtgattcaac aattatttgc acttttaaaa
    17881 tttagaaatg aatctgcggc gtttgatttg gatggaacga ttgaagtagc aacgccatca
    17941 gaatcagaaa tagtgattac acgtaaaaat aaaaacggta gtgatagtgc aacattaacg
    18001 gctaacttgg atacgaaaga atttatcatt gagtctaaag atataaaaat tttcactagt
    18061 gttgcattaa aattttaacc tattccatag aacaaaaaat cctttatact agcacttgga
    18121 tgacttatcc aagaccaata aaaaggactc atacatggat aagtataaaa caaatacagc
    18181 atttaataaa tggttttctg ccataaaatt aggaaaactt tctgtcaact cccaaaaatg
    18241 tatttttgat tacaattcgt atcgtaaaaa actgtctttt gaagctgtgc tcaagctctt
    18301 tctctacgca gttaatgaag aaaaagaaag tttgcgtgat ttgagtacag ctcttatcga
    18361 ccgatcgctc caactagaaa ccgatgtgtc atcgattagt catacccaat tgtctcgagc
    18421 atttaatgca cttgaaccta aagtgttaga agaaattttt caacaactgt tagaaaaagt
    18481 tcaacatcaa atcaaaccaa ccaagcgaaa tcatctgtat ctgattgatt ccagtacgtt
    18541 ttcgcttagt ttaaaacgtc ataagtgggc gactttccgt caaacaaaat caggtgtcaa
    18601 gttacactta aactattgtt atatggacga tactacgatg tatccaactg attttacctt
    18661 aacaaatgcc catgaacatg atctcaatca actaccagta ttggtgaatc aaccagaggc
    18721 aacttacgtg tttgatcgtg ggtattagga ctttgaaaag atggatcaga tgcattggga
    18781 tggctatttc ttcgtcactc ggattaaaaa aaacaccaaa gttcacgtga tagatactct
    18841 ggaaacatcg ccagaaacag aaattctacg agacgagctg gttcgtttag gatctaaaac
    18901 ctatctaacc gccaatttcc gactagtcac tgttcaagat aagaacggac gtgtgtttca
    18961 atttatcacc aatcgaatgg acgtctcttc aaaagaaatc tctgatatgt atcacgctcg
    19021 ttggcaaatc gaattgtttt tcaagcacat caaacaacac atgaccatta aaaccttctt
    19081 ttctcaaagt gaaaaaggcg tgcagaatca attgatttta acgatgattt ccgctctcct
    19141 cacgtttctc atcaaattgg agacgaaaac ggaaaaatca gtgttccaaa tcaaacgatt
    19201 ctttcgttat ttactgtttc agccctttga atgttgtcta gaaaaattaa ttcccacatg
    19261 agaaaaaaga aatttggctc gttacttttg gttttcagtt gaataacaga taaaaaattt
    19321 tctggaaaaa gtcatcctct gcgtcagcac agtttttttt gacttatttt cagaggaaat
    19381 agttttcaga atattctaaa atgttttatg caacactagt gatatttttt agaattagat
    19441 ttctaatgtg cattttttga gtattaagga gcaagtatac aaagatttat aaagctgatt
    19501 aaatttgaat aaaaatatgc aggaacgcta ctatttttag tagtgttctt ttttatattt
    19561 gaataaaaaa tgtggtggtt aaatggtggt tataacaaaa aatgaattaa tgaaattagg
    19621 ttatgggaag tatcagtctg agagaatcat acgacaggca aaagcaatta tggtttcaaa
    19681 aagctatacc tattacgaaa atagaagatt aggactagta cctgttgaat ctgtagaaga
    19741 agttttaggt atgaagatag aggtagagga ggattttcag aatcatggtt aattttaaaa
    19801 aagataaaaa tgggaaatat cattttactg ctagtttggg attcgacaaa cgaacaggga
    19861 aaagagtaca aaaaaacgta gaggggttaa aacagttaaa gaagaaaaaa agcgtatacc
    19921 gaacttgttt ctaattatgg aaaagaagca gaaacttctc aaagtactat gttgtttgag
    19981 gagttttttt ataaattatt cctaccttat tatcaaggga aagtagaaga aagaacatat
    20041 aaaaatcggt tatctacaat caaaaaaact tttcttattt ctttaaaatg gagttaagag
    20101 aaatacgtcc aattgacatt caaaagtggc aaaataattt attgagcaaa tataaaaatt
    20161 cttatgtaag aaacatgtat gggatttttc agatgtcatt agatcgagca gttgttttgg
    20221 gactacttga aaaaaatcct gcaaaagtta ttggaaatgt gaagaaaagg aaaactacag
    20281 ttgatttttg gacaaaagaa gaatttacaa aagttatttc aactttttat attgatgatt
    20341 tgtaccaaca ttttggtttt atttgtatgt ggttactttt tatgaccgtt atgcgtttgg
    20401 gagaagccac tgcattaact tggaaagata ttgattttga aaataaaaac ttgcgtgtga
    20461 ataaaacact ctattataag agagccaaaa cctatgagtt tgtagaaccc gaaacggctt
    20521 ctagtattcg tacaatcgaa ctagacgaca atacaattac ctttttaaaa gcttggaagg
    20581 agcgtcaagt ttcaatagga ggcatgtcgt ttgtactttc ttataatgga acacctactc
    20641 agaaatatac aatccgtcat attatgagaa gacatgcaac gatcgttggt gttcatataa
    20701 tccgcataca cggattaagg catagtcatg cttctttgct tatatcacaa taaaaagatg
    20761 cacttcgaat taaagaaagg ttaaaacatg ctgatgtaca aacgacttta ggaacttatg
    20821 gtcatttgta tctaaattca ttgagagaga taacaaatga tcccacagat tatatccaaa
    20881 ctaatttttc taaaatcaaa ccgacaccta tttacgataa taaaaaataa atgataaaca
    20941 aaagtaggat cacaccaaaa gaaacacact agaaaaacta taatacaggc ttttctagcg
    21001 tgttgtttct tatttccgcc tatttataca ttatactcat aaagcattta atgcatcgat
    21061 cgtagccgca ttgtctatat gggtatataa agttgttgta ttggtacttg aatgtcccaa
    21121 ttgttgggca gttaatacca atgaatttgt ctgttggtag agtctagtag ctaatgtatg
    21181 acgcagtttg tgagggctga cttgaattct aaagcctttt gaatattttg ctaccatccg
    21241 ttcgattgca ataccggata tacgcttggc ttctccttgg taaattgcta gaaataaagc
    21301 tttttcgttt tctgttactt tatatcgttc tttccgaata gctagataat tagctaaata
    21361 ttccattgcg aatgctgcta tattgacagc atctctttta tttccttttc tagttactac
    21421 cacattattc ttatctaaag ataggtcatc taaattaata tttataacct cggagagtct
    21481 caatccagat cctaaaatca gtgcattgat cgccaggtct ctttctacat ctcgttgata
    21541 atagatcaaa gctcgtttac tcaaagtatt tttatattca ttggcaatat aatctagata
    21601 attttgtgta tccttatcta gaaataattt tttctcaatg gcattggcac gtgcagctaa
    21661 cgtagtttta tctgtgatgg ttgctacttg atccatagga ttattgtcta agtatggttt
    21721 tcctagagga ttattggaac ttttcgaaag atatttaaat agaaccttta atgccgtgat
    21781 atttctattg atagtagaag tagatagacc taagttatta ttatctttga gcttagtttt
    21841 tgttgtctct ttttttacat tttcttttgt gcgacgtttc aaatgagatt tgtacaagtt
    21901 taggtcatag gcagttaact gtcttatcga ggtgatagaa gtatcaggaa cgatagactc
    21961 actcagaagc cattcataaa agagcttata attttttaga tattctaaag tggttcgagg
    22021 tgattgatct ttatcctcac gatcttgaac aaaatctttt atataatcgg gcatatgaag
    22081 tatgattgta tctatttttt gatatatttc tgtatttttc atgtcattca tcctttcaat
    22141 aagtagggga gaagagtatc tctataattg gactatacca attatagaat gttttgtcaa
    22201 ttggtatgct ctaaaattta ttaatttgtt tcaaaaaaag catataaaca ggtatacgtt
    22261 taataattta aaataaatgg tatacataaa tagggaagga aatagaaggg agcaatctaa
    22321 attttttgaa ttttcttaat taattatata gaaagaacaa tctttctata gagttagaca
    22381 tgagagggac tatgcttgat ttaaaatcaa caagcgtata tactgtatat acaaagaaaa
    22441 aggagggaga agactatggc aattaaagaa agaaaattta gggaagtggg gaactcagta
    22501 gtattaacta tttcaaagga gtttcttaaa gaaaatgggt taaatcccgg agatacggtt
    22561 tttttagatg aagaaaaact tcgagcggca attacaaaaa aacaagcctt atctgatgaa
    22621 attgatttag caatatctca agcattaact gaatatgata ctacttttag agagcttgtc
    22681 gataaatgag tattcattac ctagaagcca aagatattat aaaaatgaat gtaatccaaa
    22741 taaaaaaata ctctcctaat gaaccccttg gtgtaaagga tgcgaatgct ttagaaatgt
    22801 gtgtagggca acttcgagcc tcagcttttg gtgaagaggt ttatccttcg atttatgaaa
    22861 aagcagctat cctacttata caattgataa agaaacatcc ttttcacaat gcaaataaac
    22921 gtactgcttt cttagctact tttgtctttt tgaaaatcaa tggacagctt ttagctattg
    22981 atcaaaaaga tgctgtaaat ttagcggtat atattgctac gtatgataag gatttcgatc
    23041 aactaaaata tgaagtgatc aacactataa agacacatac gcagcccatt taaggagtta
    23101 ctgtatgtgt ctttataggg ttattagctg ctatagcgaa ataattgaag taaattacaa
    23161 atagctataa ttcaaatcca actatataga aaacgtaaat tttctatata gttggattaa
    23221 gataacgact atttatttta tcatattttt actataccct atacttttac ttggtatatt
    23281 tatgaataat cgcaaattta cccctatact actagtttgt aagttagatt aataaaaata
    23341 ctaaagttat ttgttctttt attaaataca cctacaactt ataaattgat ggtatttaga
    23401 taacacgaaa gaataaaaat aacagctgat agtaaagttt gttgacgatt gctactcaat
    23461 cgaaagtgag ttagtgcgta tgaacttgct tctgaaaact tatgaaatga aagacctttt
    23521 acatcttgca gaaatcggta ctgggattaa actaagcaat aaaaactaat gttcccctca
    23581 ttctttgaac ttctactatt tgatttattt attctgataa atgacgattt caccttcagg
    23641 atcttcaaat cccgtataga atttatttaa gatctcttca gtatcagtaa taggataggc
    23701 gataggccac ataccctttg atggttagaa taaactgctc aaaatctgta tcgtatgttg
    23761 gctttagcat attaacaaag gctatctcca aattataaac cataagcgtt gaatcgttct
    23821 taacaatttc aattcttcct aacccaaagt cagaagatat aattaccgga cttgccttac
    23881 ccttcatgtg ggttgttgag attccatatt taaagggaat tgtcggtact atcggcggtc
    23941 tgaaaaacat cccaaaaata aaaaaatctt tcccctgcta gtctcctaag tccctttgta
    24001 gcggatatgg caccataatc tcattttatc gaagtgagtc atggtttatc tgttggccaa
    24061 ccaaaattga tacaatctaa cgatgcaccc tctacggcat tctaggggag tgcacttgca
    24121 ttcacccaat aaaaaccggc agttagcaaa gaacttatgt agtcgtatgt tcttactgtt
    24181 aacatacaag aggtctcctt tccacaattg cttagcagta aacgggaatt tacaaagcta
    24241 atctcttcca ttttcttatc tttgttctaa aagtcccaaa tggagcaact gtatttacat
    24301 gaataaattt atatacttcc catacagccg ttttggttgc ctcgtctgcc cattttcttt
    24361 tgtgtgcagt gaatagatct tcatcactca ttgaatcaat catcatgtta atatcatcaa
    24421 tattacgatt gagaatttcc tctaattctt tcaatggtag atgtgcatag gtatttgtaa
    24481 accacttata taactctcct aactgattcc atttaaattg atcagaaggc gttttaacag
    24541 ataatccatt tctttcatca ttttcccatt ttagaattaa attcgtccaa ccaacttgat
    24601 atgctaaatt ttcagcaggt gttctatcca cttcctcaac tcttttatcc ttcaattctt
    24661 caggaatagt ttcaaattct aaaatatatt tagtataagt cctatttatt tcttttttta
    24721 attcttcttt atttttatat gttctcatta tattacctcc ataaattcct aattgtctcc
    24781 tttaattcat ctataatttc atccacgata ttttaatccg ttctacaatg catatctgat
    24841 atgcattgac tgtctaactc atatgtgaat gatttataaa atatcccaac gtctcatcta
    24901 aagaaatgtt gatcgtcagc aaggagctaa aaaatacgaa tatatttctt ttgtagtaaa
    24961 atcggactgg tttgcatatc attttcttcg taaatatcaa aatcatgtta actactatca
    25021 attactaata aaaactacca ctttaagcgt taagaactgt taagtttagt taagtgagga
    25081 tagttcacaa aatccattgt taaacttcac gaaagtggta aaactcaaaa tgctttatat
    25141 aggattacag catcgctctt tcttcgattg cgcgttgggt aaaaaatatt atgaagtaaa
    25201 aattgacgat gacacgttcc ttacggctca tcagatgaaa cagctacaaa aacgaaatga
    25261 tcaactcgaa gaaaatttaa ttctaaaaaa gcgattgcca tattcacgcc tcgctcgacg
    25321 aactattaaa ggtcaagatt tacctgaaga tcatcctttt taggaaattg aagcttttta
    25381 tgttgcacaa tgcatttata atactacaat gatgttttca ccagatattc ttctagggtg
    25441 cggtgtaatg aaacagatac acttgcaaaa gaaaattcac cgcgaattta ctaaaattat
    25501 gaataattat gttaatcttc cgaaattttt ttccaaaatt aagggatcaa gctggtgtta
    25561 tagattgttt aaggttggca aaaaaataac ttctttttaa acaattatag aaaaccgata
    25621 ctctgttaag ttggttgaca cggtgtcgat ttttatgact ttcttattct gaaacttcac
    25681 taacggacag tacctaaaaa gtctatagta tggacgttta cgagtactat tttatcaaaa
    25741 atgctgctca tttcaactta tatgttttag tgaactaccc ccactta
//
LOCUS       DACEEZ010000032        25210 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20313_2017
            SAMN12639966-rid9879223.denovo.032, whole genome shotgun sequence.
ACCESSION   DACEEZ010000032 DACEEZ010000000
VERSION     DACEEZ010000032.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639966
            Sequence Read Archive: SRR10023158
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 25210)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25210)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741683.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741683.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:44:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,016
            CDSs (total)                      :: 2,962
            Genes (coding)                    :: 2,889
            CDSs (with protein)               :: 2,889
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 36 of 73
            Pseudo Genes (incomplete)         :: 29 of 73
            Pseudo Genes (internal stop)      :: 18 of 73
            Pseudo Genes (multiple problems)  :: 8 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25210
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639966-rid9879223.denovo.032"
                     /isolate="E20313_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            115..762
                     /locus_tag="IWO89_001374"
     CDS             115..762
                     /locus_tag="IWO89_001374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensing transcriptional repressor Rex"
                     /protein_id="HAQ0456137.1"
                     /translation="MREKTDTLPKATAKRLPLYLRYLKMLDDSGISRIKSNEFSEITQ
                     IPSATIRRDFSQLGELGRSGYGYDVPFLIDVFNNILNTKEEKRIALVGYGNLGKALKH
                     NNFRRNENLNIVCVFDNDPALINRVIDGEMIYPIDRFAEIAKAKNVTVAISTVPSKYS
                     QSAIDEIVKGNVTAILNFAPDRVTVPAYVNVQYIDLTTELQTLIYFDENYSEVFS"
     gene            1155..3800
                     /locus_tag="IWO89_001375"
     CDS             1155..3800
                     /locus_tag="IWO89_001375"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HAQ0456138.1"
                     /translation="MTEENNLSTKYNPQEVEAGRYQHWLEQDLFKPSGDKKAKPYSIV
                     IPPPNVTGKLHLGHAWDTTLQDMLIRQKRMQGYDTLWLPGMDHAGIATQAKVEAKLRE
                     QGISRYDLGREKFIDQVWEWKEEYASHIREQWAKMGLSLDYSRERFTLDDGLSEAVRK
                     VFVTLYEKGLIYRGEYIINWDPQARTALSDIEVIHKDIEGAFYHMSYELADGSGVVEI
                     ATTRPETMLGDTAIAVHPEDERYQALIGKKVILPLVNKQIPIIADEYVDMEFGTGVVK
                     ITPAHDPNDFEVGNRHDLPRVNVMDDDGTMNELAGKYAGMERFAARKAIVQDLKEIGR
                     LIKIDTMIHSVGHSERTGVVVEPRLSTQWFVKMAPLAKKAIDNQETDQAVEFFPPRFN
                     QTFLRWMENVHDWVISRQLWWGHQIPAWYHKETGEMYVGMEAPADSENWVQDPDVLDT
                     WFSSALWPFSTMGWPDTESEDYKRYFPTSTLVTGYDIIFFWVSRMIFQSLEFTGERPF
                     ENVLIHGLIRDEQGRKMSKSLGNGIDPMEVIEKYGADALRWFLSNGSAPGQDVRFSYE
                     KMDASWNFINKIWNASRFVIMNIEGMTVEEIDLSGEKSVADRWILTRLNETIERVTEL
                     FDRFEFGEAGRQLYNFIWDDFCDWYIEMSKEVLYGDDAVSKQTTRSILVHTLDQILRL
                     LHPIMPFVTEEIWEHIPHQGNSLVVAEYPVVRPEFDDETASKGMEVLKELIRAVRNIR
                     SEVNTPLSKPITLLIKTNDAKIDQFLVENTSYIERFCNPEELTISSEITAPDLAMSAV
                     LTGAEIFLPLAGLINIEEEIKRLEKELEKWTSEVKRVQGKLSNERFVSNAPEEVVEAE
                     RAKEKDYLEKQTAVKERIESLRTIQ"
     gene            4110..5423
                     /locus_tag="IWO89_001376"
     CDS             4110..5423
                     /locus_tag="IWO89_001376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional folylpolyglutamate
                     synthase/dihydrofolate synthase"
                     /protein_id="HAQ0456139.1"
                     /translation="MTIEEAIEWIHSRLPFGSRPGLDRVEALLEVVGHPERKVKTIHI
                     AGTNGKGSTVAYLRSLLEEAGLTVGTFTSPYIETFNERIAINGKSIPDNDLIQLVKKY
                     QPLIKQLDQFEAVAGITEFETLTAMAFDYFLMKKVDVAIIEVGLGGLLDSTNVAVPML
                     TGITTIGLDHTEILGDTIEEIAAQKAGIIKQGVPVVTGNIAPEAFTVIQQTAKAKNAP
                     LYVYGENYKINYLHPDEMWGEVFEFFNEIGKIPQLTTPLLGQHQAENAGVAIELYFVY
                     CQLEKLPFREKDVLKGLKKSRWPGRMERLSNEPLVVLDGAHNTHAMNRLVENLKKEFK
                     GYQINILFSALRTKNVREMLERLQQVPNVHIYLTTFEHPKAIELSEVSDLASERVSIV
                     SLWQFGLADILEKMTNEDVLVVTGSLYFISEVRHLLIQLGGDHEV"
     gene            5413..6066
                     /locus_tag="IWO89_001377"
     CDS             5413..6066
                     /locus_tag="IWO89_001377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HAQ0456140.1"
                     /translation="MKYNAVIFDMDGLLFDTELVYYEATQVVADKMGFPYDKDLYLKF
                     LGVSDEEVWANYHMIFSEFGKTSVQRFIDDSYQETLERFSQGAVQMKPGVLELLSFLE
                     EHQIPKVVASSNQRAVIELLLKKNNLFERFETIVSAENVQRAKPDLEIFLLANQKLGT
                     AKKETLILEDSQNGILAASAAEIPVVMVPDLLPPSQELIKKTAGVVSSLHEIPALLK"
     gene            6121..6672
                     /gene="radC"
                     /locus_tag="IWO89_001378"
                     /pseudo
     CDS             6121..6672
                     /gene="radC"
                     /locus_tag="IWO89_001378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736077.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadC"
     gene            6803..7888
                     /locus_tag="IWO89_001379"
     CDS             6803..7888
                     /locus_tag="IWO89_001379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HAQ0456141.1"
                     /translation="MKVQRIEVENKPYPLYLLLDKEYQLIEPVMKFIKYLDNTGKSPN
                     TIKAYCYHLKLLYEFMEQRGVILNDINFELLADFVGWLRYPSASNVIDLQSKKAIREE
                     TTVNTILNVVMSFLDYLSRLGEFKSIDVFKQAKGRNFKGFLHHVNKGRYQKNVLKLRV
                     KKKQIRTLRSEEVKQIIDACHTKRDKLILMLMYEGGLRIGEVLSLRLEDIVTWDNQIH
                     LTPRDVNVNEAYIKLRKERTIHVSKELMSLYTDYLIYEYSEELEHDYVFISLKEGYFG
                     KPLKYQSVLDLVRRIVKRTGIEFTSHMLRHTHATQLIREGWDVAFVQKRLGHAHVQTT
                     LNTYVHLSDQDMKNEFNKYLERKEHKK"
     gene            7885..9777
                     /locus_tag="IWO89_001380"
     CDS             7885..9777
                     /locus_tag="IWO89_001380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001833801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HAQ0456142.1"
                     /translation="MNASSKRKIISQSEISKKIAVMNEEMQGFWANNSWDIRKCPHPS
                     AIELSKNPALRNRWVRFERVKNLWLRTELKYFYFYHLNNGIWNAKTVWIRKGTVINKM
                     LDFLDLKYPSITSITEVPIEKAMTEYRTYLTKRGVRITTTNYKITANQEKTPVKANSY
                     YVTNLKQFMEFYENFYFDGEEWDKDVWDRRNLPLPDDKVNPTQYEYTINFKGFRNTYF
                     KQLVKRYCKLRLNMDSFSYVSDIAQRLKEFFNFLDMKFKQVQRVHQLTRVEIEAYLSE
                     LNMMGIKPSTITGRISILEGLFSTLLRLEWDDVPSKILIYSEDYPKIPRAKPRFIDEF
                     VLEQLNSHLDKLPEYIATMTMIVQECGMRISELCTLKKGCLLEDKDGDFFLKYYQWKM
                     KKEHIVPISKEVALLIKVREDKVSEEFPDSEYLFPRKDGSPLKQETFRGELNKLAYEQ
                     NIVDKSGEIYRFHAHAFRHTVGTRMINNGMPQHIVQKFLGHESPEMTSRYAHIFDETL
                     KNEFTKFQEKLVTNNGDVLDLDEDNEVDDVELQWFKKNINAQVLPNGYCRLPVVAGGC
                     PHANACLDCTHFCTSKQFLPQHEEQLERTEELLAIAKDKQWQRQVETNSRVKERLEQI
                     IGSLTG"
     gene            9784..10161
                     /locus_tag="IWO89_001381"
     CDS             9784..10161
                     /locus_tag="IWO89_001381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000361059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HAQ0456143.1"
                     /translation="MDKQVRNTTEIVRLAKQKSKKTREKVDKAISKFSIEGKVINFNS
                     IAKEANVSKSWLYKEHDIRQRIESLRERQITANVVSKPKKSSRSEEILIKTLKRRVME
                     LEKENKKLQNQIQKLYGDLYNKE"
     gene            10315..11097
                     /locus_tag="IWO89_001382"
     CDS             10315..11097
                     /locus_tag="IWO89_001382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000067268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside nucleotidyltransferase ANT(9)-Ia"
                     /protein_id="HAQ0456144.1"
                     /translation="MSNLINGKIPNQAIQTLKIVKDLFGSSIVGVYLFGSAVNGGLRI
                     NSDVDVLVVVNHSLPQLTRKKLTERLMTISGKIGNTDSVRPLEVTVINRSEVVPWQYP
                     PKREFIYGEWLRGEFENGQIQEPSYDPDLAIVLAQARKNSISLFGPDSSSILVSVPLT
                     DIRRAIKDSLPELIEGIKGDERNVILTLARMWQTVTTGEITSKDVAAEWAIPLLPKEH
                     VTLLDIARKGYRGECDDKWEGLYSKVKALVKYMKNSIETSLN"
     gene            complement(11223..11954)
                     /gene="erm(A)"
                     /locus_tag="IWO89_001383"
     CDS             complement(11223..11954)
                     /gene="erm(A)"
                     /locus_tag="IWO89_001383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001072197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2058)-N(6))-methyltransferase
                     Erm(A)"
                     /protein_id="HAQ0456145.1"
                     /translation="MNQKNPKDTQNFITSKKHVKEILNHTNISKQDNVIEIGSGKGHF
                     TKELVKMSRSVTAIEIDGGLCQVTKEAVNPSENIKVIQTDILKFSFPKHINYKIYGNI
                     PYNISTDIVKRITFESQAKYSYLIVEKGFAKRLQNLQRALGLLLMVEMDIKMLKKVPP
                     LYFHPKPSVDSVLIVLERHQPLISKKDYKKYRSFVYKWVNREYRVLFTKNQFRQALKH
                     ANVTNINKLSKEQFLSIFNSYKLFH"
     gene            complement(12012..12099)
                     /locus_tag="IWO89_001384"
                     /pseudo
     CDS             complement(12012..12099)
                     /locus_tag="IWO89_001384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017771940.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ErmCL family antibiotic resistance leader
                     peptide"
     gene            12463..13125
                     /locus_tag="IWO89_001385"
     CDS             12463..13125
                     /locus_tag="IWO89_001385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HAQ0456146.1"
                     /translation="MNTIESQFDKVAEDYDFVNELLNDYSFFVSNMSPKKGRALDIGC
                     GSGLLVEKLASYYDEVVGIDISNQMLDLAKSKRQLTNTVYLNMNAEQLNFNEKFDFIV
                     SRTTFHHLDDIASVIQQMKELLNEEGRIVILDNVSEVETPPTYVYKLGAIQEFLPHCF
                     KFGIKNAIRIYNHNTSKSWLEHLASDKYLSEQNYYDLYEKLLPGCQFHKMGWAMGVVW
                     TK"
     gene            13360..13503
                     /locus_tag="IWO89_001386"
                     /pseudo
     CDS             13360..13503
                     /locus_tag="IWO89_001386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326507.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein"
     gene            13653..13853
                     /locus_tag="IWO89_001387"
     CDS             13653..13853
                     /locus_tag="IWO89_001387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HAQ0456147.1"
                     /translation="MENGTVKWFNAEKGFGFISREDGSDVFVHFSAIQGDGFKTLEEG
                     QAVTFDVEESDRGPQAANVVKA"
     gene            complement(14107..15786)
                     /locus_tag="IWO89_001388"
     CDS             complement(14107..15786)
                     /locus_tag="IWO89_001388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease J"
                     /protein_id="HAQ0456148.1"
                     /translation="MKLSIKNNETGVFAIGGLGEIGKNTYGVQFQDEIILIDAGIKFP
                     EDDLLGIDYVIPDYSYIIQNLHKVKALVITHGHEDHIGGIPYLLKQANIPIYAGPLAL
                     ALITNKLDEHGLLRDAELHEINEDTIIRFRKTSVSFFRTTHSIPDALGIVVKTPSGNI
                     VATGDFKFDFTPVGEPANLHKMARIGEEGVLCLLSDSTNAEIPTFTKSEKTIGHSILK
                     IFSKIEGRIIFASFASNIFRLQQAADAAVATGRKIAVFGRSMENAIVNGQRLGYIKVP
                     DGTFVDAHEINRLPANETMILCTGSQGEAMAALSRIANGTHRQIQIQPDDTVIFSSSP
                     IPGNTTSVNHLINLLSEAGAEVIHGKINNIHTSGHGGQEEQKLMLRLMKPKYFIPVHG
                     EFRMLKVHASLAQDVGVPAENCFIMENGDVLALTSDSARPAGHFVAGDVYIDGSGIGD
                     IGNVVLRDRHLLSEEGLVLAVATVDIKKKEILAGPDILSRGFVYMRESGEMINEAQRI
                     LFHALRECLNGPDCSEYKLKEAMTGALQPFLFERTERHPMILPMVMTPDEE"
     gene            complement(15788..16000)
                     /locus_tag="IWO89_001389"
     CDS             complement(15788..16000)
                     /locus_tag="IWO89_001389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-dependent RNA polymerase auxiliary subunit
                     epsilon family protein"
                     /protein_id="HAQ0456149.1"
                     /translation="MIYKVYYQETKVRNPKREETKSLYIEAKSDVDARQRVEENTPYN
                     IEFVQLLEGSHLEYEKENADFSLVEF"
     gene            16393..18123
                     /gene="efrA"
                     /locus_tag="IWO89_001390"
     CDS             16393..18123
                     /gene="efrA"
                     /locus_tag="IWO89_001390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux ABC transporter subunit EfrA"
                     /protein_id="HAQ0456150.1"
                     /translation="MKLMWHYTMRYKKFLFFNFICVFGFILIELGLPTILARMIDVGI
                     RNDDFDYVKQQGIFMIVITVIGIILNILLGYFGSRITTNIVADIRDDLFEKIQSYSHQ
                     EYENLGVSSLITRTTNDAYQIMLFLQNILRIGFMAPMMFIVSLYMVMRTSVTLSLYVV
                     GALPLLLLAVVAIAKVSEPLSKKQQKNLDKINSILRENLSGLRVIRAFVNEKFEEKRF
                     AVVNENYASSSKSLFRLMAVAQPGFYFLFNIVMVLIIWSGAVQIDAGELRVGDLIAFI
                     EYIFHALFSFMLFASVFMMYPRAAVSAGRIQEALDAKPSIVEDPNGISDTKTKGYIEF
                     QNVTFAYPGHSQEPVIRNVSFTASPGETVAFIGSTGSGKSTLIQLIPRFYDVSEGKIL
                     IDGVDVRDYRLSKLRDKIGFIPQKALLFTGTIADNLRYGKEDATQEEMERAAEIAQAS
                     EFISRKPDGFNEHLSEGGSNFSGGQKQRLAIARAIIRRPEIYIFDDSFSALDYQTDAK
                     LRARLKKETTESTVLIVAQRVGTIMHADKIIVLNEGEVVGMGTHRELLEKCPIYYDIA
                     ASQLSKEELA"
     gene            18120..19880
                     /gene="efrB"
                     /locus_tag="IWO89_001391"
     CDS             18120..19880
                     /gene="efrB"
                     /locus_tag="IWO89_001391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_029485765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux ABC transporter subunit EfrB"
                     /protein_id="HAQ0456151.1"
                     /translation="MKKTFSSLKRLARYIRPYRETFLLVILFTVLTVAFNVALPYVTG
                     LPTTEISRNIANGESINFSYIFKCLIWITVVGIGYCISQLLSGVLMTNVVQSAMHDLR
                     RDIEEKINRLPVSYFDKNQQGNILSRVTNDVDAVSGALQQAFIGIVNAILGITLAAAM
                     MFYIQPMMALISMIMIPTSIWISKRVINASQKYFQSMQNSLGELNGYVQENMTGFSVL
                     KVYGREKETLEGFKKVNHSLKYFGFRAAFISGLMMPLVQLTAYATYIGMAVLGSFYVI
                     TGAIVVGQLQAFIQYIWQISQPMGNVTQLSSVLQSASAATKRVFEILDEPEEKENKVD
                     ASLPETILGDVTFDHVDFAYDPKKPLIQDLNFEVKAGQTVAVVGPTGAGKTTLINLLM
                     RFYDVDKGAIRIDGIDTKSMSRSDVRSLFGMVLQDAWLYQGTIADNIRFGKLDATDYE
                     VVDAAKTANVDHFIRTMPDGYDMAINSEGDNVSLGQKQLLTIARAVVSDPKILILDEA
                     TSSVDTRLEALIQKAMDRVMEGRTSFVIAHRLSTIRDADLILVMDQGQIIEKGTHESL
                     LAQGGFYEKLYNSQFAEEAE"
     gene            19975..20172
                     /locus_tag="IWO89_001392"
     CDS             19975..20172
                     /locus_tag="IWO89_001392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0456152.1"
                     /translation="MEQFLNEKITEAKREVDRLTHVCQTKPFDSADFFENEFQIERLA
                     AQIEVYELILKKVIEENIDVQ"
     gene            20325..20810
                     /locus_tag="IWO89_001393"
     CDS             20325..20810
                     /locus_tag="IWO89_001393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma carbonic anhydrase family protein"
                     /protein_id="HAQ0456153.1"
                     /translation="MERFIASNATVIGDVTLSEDVTIWYQAVLRGDSNWIKIGQRTNI
                     QDGTIIHVDHDAPVDIAENVTVGHQCMLHGCTIEKGALIGMGTTILNHAVIGENSLIG
                     AGSLVTEGKVIPPNVLAFGRPAKVIRPLTKEEIQKNKENIQHYVEIGRMHAQGKFKEQ
                     R"
     gene            complement(20914..21507)
                     /gene="clpP"
                     /locus_tag="IWO89_001394"
     CDS             complement(20914..21507)
                     /gene="clpP"
                     /locus_tag="IWO89_001394"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp endopeptidase proteolytic
                     subunit ClpP"
                     /protein_id="HAQ0456154.1"
                     /translation="MNLIPTVIEQSSRGERAYDIYSRLLKDRIIMLSGQVTDDLANSI
                     IAQLLFLDAQDSEKDIYLYINSPGGSVTAGMAIYDTMNFVKADVQTIVMGMAASMGSF
                     LLTAGTKGKRFALPNAEIMIHQPLGGAQGQATEIEIAARHILQTRERLNKILAERTGQ
                     PLEVIEKDTDRDNYMTAEQAKAYGLIDEVMENSSSLN"
     gene            21687..22214
                     /locus_tag="IWO89_001395"
     CDS             21687..22214
                     /locus_tag="IWO89_001395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CAP domain-containing protein"
                     /protein_id="HAQ0456155.1"
                     /translation="MIVGITGLIGVLTGAVVVYIGMILQMRHLENKEQRRRELELKVK
                     EIETLNQLNKKINEILQKRTTLMEEYVSFDAFDDCYITIDDFAYLQSFSAQNSFYLPN
                     YILDELFKNISHRKVILSPDETMKIGGYAYKGGRVILENFTDSLTEMINEKKAQLKTM
                     TRQPIGFFSKDDVWS"
     gene            22305..23042
                     /locus_tag="IWO89_001396"
     CDS             22305..23042
                     /locus_tag="IWO89_001396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HAQ0456156.1"
                     /translation="MLQIYAHRGSSGTHPENTLPAFAEAVRVGADGIELDVHLSKDGY
                     LIVMHDEEVDRTTNGKGLIREKTLEELKKLNAGGKFSKEFPAAKVPELREVLGLLLQK
                     NYRGMLMIEIKTDQYEYPGIEEKLAETLKSRDWPFQHAYCSFHLDSLEKIALLEPEIQ
                     LDLLMSTSDKKIQLAAETPYIEGVHPKNTWLFEHPVQAADFPKAIRAWTINDEEEMAK
                     CQELKIDGIITDFPEKALRWKQSTKSR"
     gene            23046..23963
                     /gene="miaA"
                     /locus_tag="IWO89_001397"
     CDS             23046..23963
                     /gene="miaA"
                     /locus_tag="IWO89_001397"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HAQ0456157.1"
                     /translation="MEKVLVIVGPTAVGKTALSVELAQLFGGEIISGDSLQIYKNLDI
                     GTAKIKEEEMNNVPHHLIDVIEPTQRYSAADFQHSGRQLITEIANRGHLPIIAGGTGL
                     YIQSLLYDYQLGATEEANPEIRKKYETLAETIGSQALWNQLKEKDPAAAEKIHWNNQR
                     KVIRALEVLETTGHSITAPKEEPKQLYDYLMIGLDTKRSILYERINQRVDLMLAEGLV
                     AEARKVYELGEVQASQGIGYKEFFPYFEGKETLEEATEQVKLHSRRYAKRQLTWFRNR
                     LDAQWFDLISEPNQKSQIEQTIKKWLEAE"
     gene            23965..25194
                     /gene="hflX"
                     /locus_tag="IWO89_001398"
     CDS             23965..25194
                     /gene="hflX"
                     /locus_tag="IWO89_001398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HAQ0456158.1"
                     /translation="MEEKVIIVGVETEANQRYFSESMEELVQLTNTASGEVVFTITQK
                     RPQVDRQTIIGKGKLQELVQQADAHEADLIIFNHELTPRQSQLITDAVGLPVIDRVQL
                     ILDIFAMRARSKEGKLQVELAQLEYLLPRLVGQGKTLSRLGGGIGTRGPGETKLETDR
                     RHIRNKIFAVKKELKEVEAHRERNRQKRKNSEIFQIGLIGYTNAGKSTILNLLTQADT
                     YSKDQLFATLDPLTKRWRFAEGFEITVTDTVGFIQDLPTQLIDAFHSTLEESQNMDLL
                     LHVVDASSPDRILQEQTVLKLMDELNMKEMPILTVYNKADQIDPAMFTPTLFPNVLIS
                     TQTKEGKMALIEAIKRQLMELMTPYTLSVPSNEGQKLSELRRQTMVLDEAYMEETNEY
                     EVKGFAKTNSKWLRKNQ"
BASE COUNT         8393 a         4105 c         5299 g         7413 t
ORIGIN      
        1 ttttgaacac cgttttattt gtctcacacc cttttccttc ctacttgtaa ccttgctccc
       61 gtgttagaaa catgttacac taaatatgtg aaattattaa caaggagcgt cgttatgcga
      121 gaaaaaacag atacactgcc aaaagcaact gcgaaaagat tacctttata tttacgctac
      181 ttgaagatgc tggatgactc aggtataagt agaatcaaat caaatgagtt cagcgaaatc
      241 acccaaattc cttctgcaac gatccgcaga gacttctcgc aattaggcga gctaggcaga
      301 agcggatatg gctatgatgt cccttttctt atcgatgtat tcaataatat ccttaatact
      361 aaagaagaaa aacgaatcgc attagtagga tatggaaatc ttggcaaagc attgaaacac
      421 aacaatttta gaagaaatga aaatctaaat atcgtgtgcg tctttgataa tgatccagca
      481 ctgatcaatc gagtaattga tggagaaatg atctatccga ttgatcgttt cgcggagata
      541 gccaaagcaa aaaatgtgac agtagcaatc tctacggtac caagtaaata ttcacaatct
      601 gctattgatg aaattgtcaa aggaaatgta accgctatac ttaattttgc gcctgacaga
      661 gtaactgtcc cagcttatgt caatgttcaa tacattgatt tgacaaccga gttgcagacg
      721 cttatttact ttgatgagaa ttactcagaa gttttttctt aggaaaattt gagtaggctt
      781 attgatcttg cgagaactcg tcgtaattct ttcaaaaatt ttttagtact aaatgaagtg
      841 gttttttcac gaccacttgt tgactaaaaa aaaaaaccat tgtagaatga agcataacgc
      901 tgaacaagag gagtaactgg cacattcatt cttagagaga gaactatctg ctgaaaggtt
      961 ctcaatgaca accagtgaag gtagcttggg agtttctgtc ttgaatggag tagagataga
     1021 cgagtcgtga tcgttaactc acttgagagg caataatgat tgattattgc aatttaggtg
     1081 gtaccgcgta aatcatacgt cctagaatac gaaagtattt taggacgttt tttagtttaa
     1141 aggaggaaaa cagcatgacg gaagaaaata acctatcaac aaaatataat ccgcaagaag
     1201 ttgaagctgg acgttatcag cattggttag aacaagattt gtttaaaccg agcggagata
     1261 aaaaagcaaa accttattca atcgtaatcc cgcccccaaa tgttactgga aaacttcatt
     1321 tagggcatgc ttgggatacg accttgcaag atatgctgat tcgtcaaaag agaatgcaag
     1381 gatatgatac tttatggctg cctggtatgg accatgcagg aatcgcgaca caagcaaaag
     1441 tagaagcgaa gcttagagag caagggattt ctcgttatga tctaggtcgt gaaaagttca
     1501 tcgatcaagt gtgggaatgg aaagaagagt atgcgagcca catccgcgaa cagtgggcta
     1561 aaatgggctt atctttagat tacagtaggg aacgttttac attagatgat ggattatctg
     1621 aagcagtacg taaagttttt gttaccttat acgaaaaagg tttgatttat cgtggtgaat
     1681 atatcatcaa ctgggatcct caagcacgaa cagctctatc tgatatcgaa gttattcata
     1741 aagatatcga aggcgccttt tatcatatga gctatgaact agcagatgga tcaggcgtag
     1801 tagaaattgc gacaacccgt ccagaaacga tgttgggaga tacggcgatt gctgttcatc
     1861 cagaagacga gcgttatcaa gcgctcatcg gcaaaaaagt gattctacct ttagtcaata
     1921 aacaaatccc aatcattgca gatgaatatg tagatatgga attcgggaca ggtgtagtaa
     1981 aaatcactcc agcacatgat ccaaacgact ttgaggtagg taatcgtcat gatcttccac
     2041 gcgtaaatgt tatggatgat gatggtacga tgaatgaact agcaggaaaa tatgctggaa
     2101 tggaacgttt tgctgctcgt aaagcaatcg tgcaagatct gaaagaaatc ggtcgattga
     2161 ttaaaatcga tacgatgatc cacagtgtgg gtcattccga gcggacaggt gtcgttgttg
     2221 aacctcggct gtctactcaa tggtttgtaa aaatggcgcc gttagcgaaa aaagccattg
     2281 ataaccaaga aacagatcaa gcagtggaat tcttccctcc aagattcaat caaacgtttt
     2341 tacgttggat ggaaaatgtc catgattggg taatctcacg acaattatgg tggggacatc
     2401 aaattcctgc atggtatcac aaagaaacag gtgaaatgta tgtagggatg gaagcaccag
     2461 cggatagtga aaactgggta caagatcctg atgttttaga cacttggttt agttcagctt
     2521 tgtggccttt ctcaacaatg ggctggccag atacggaaag cgaagattat aagcgatact
     2581 tccctactag cactttagta accggttatg acatcatttt cttctgggta agcagaatga
     2641 ttttccaaag cttggaattt actggcgaac gtccatttga gaatgtcttg attcatggat
     2701 tgattcgtga cgaacaaggg cggaagatga gtaaatcatt aggtaatgga atcgatccga
     2761 tggaagtaat cgaaaagtat ggagcagatg ctctccgctg gttcttatcg aatggttctg
     2821 caccaggaca agatgtccgc ttcagctatg aaaaaatgga tgcttcttgg aactttatca
     2881 acaaaatctg gaatgcttca cgtttcgtta tcatgaatat cgaaggaatg accgtagaag
     2941 agatcgattt atctggagaa aaatcagtgg ctgaccggtg gatccttact cgtttgaatg
     3001 aaacgatcga acgagtaact gagctatttg atcgttttga atttggtgaa gcaggtcgac
     3061 aactgtataa ctttatctgg gatgattttt gtgactggta catcgaaatg agtaaagaag
     3121 tactttatgg agatgatgct gtttcaaaac aaaccacccg tagcatctta gtccatacac
     3181 ttgatcagat cctacgttta ttgcatccaa tcatgccatt cgttacagaa gaaatatggg
     3241 aacatattcc tcatcaggga aattctctag tagtggcaga atatcctgtt gtacgtccag
     3301 agtttgatga tgaaacagct tctaaaggga tggaagtatt gaaagaattg attcgagccg
     3361 ttcgaaatat tcgttcagaa gtgaatacac cattgtctaa accaattacg ttgctaatta
     3421 aaacaaacga tgctaaaatc gatcaatttt tagttgaaaa tacaagctat atcgaacgct
     3481 tctgtaatcc cgaagagttg acgatatcaa gtgagatcac tgcgccagat ctagcgatgt
     3541 cagctgttct gacaggtgca gaaatcttct tgccgttagc aggattgatc aatattgaag
     3601 aagaaatcaa acgattagaa aaagaactgg aaaagtggac aagcgaagta aaacgtgtcc
     3661 aaggaaaact ttctaatgaa cgttttgttt ctaacgcgcc ggaagaagta gtagaggcag
     3721 agcgtgcaaa agaaaaagat tatttagaga agcaaacggc tgtcaaagaa cgaatcgaaa
     3781 gtttgcgtac tattcaataa gaatagaaaa agagagtgtg aaatcagcta gctattacta
     3841 aaagctagct gattgaacat cgttacagga aagagtttga gcctcactct tagcttcgat
     3901 tcataaggag atgtgacata aattttgtcg catctctttt ttagtctact cttattatgt
     3961 aggagaagga gggaggtgga tcatgtatca agagtcaata aagtagttga taaaatcgtt
     4021 cctctctctg tttcttttct ctttgtcata cttttatttt tcttatgtgt tctatttgtt
     4081 ataataaaag agaaatgggg gaattgttga tgacgattga agaagcaata gaatggatcc
     4141 atagtcgatt accatttggt tcacgtcctg gccttgaccg agtcgaagct ttattagaag
     4201 tggtagggca tccagagaga aaagtaaaaa cgatccatat cgctggaaca aacggaaaag
     4261 gttctactgt agcttatttg agaagcctat tagaagaggc tgggctgaca gtaggtacat
     4321 ttacttcacc ttatatcgaa acgttcaatg aacggatcgc aatcaatggc aaaagcattc
     4381 cagacaatga tttgatccag cttgtaaaaa aatatcagcc actgatcaaa caacttgatc
     4441 aatttgaagc agtagctggt attactgaat tcgagacatt aacagcaatg gcatttgatt
     4501 attttttgat gaagaaagtg gacgtagcga ttatcgaagt tggcttaggc ggcttattgg
     4561 attctaccaa tgtagctgta ccaatgctga cgggaataac aacgattgga cttgatcata
     4621 ccgaaatact tggagataca attgaagaaa tcgctgcaca aaaagctgga atcatcaaac
     4681 aaggcgttcc agtagtaaca ggaaatattg ccccagaagc ttttactgtc atccaacaaa
     4741 cagctaaagc aaaaaatgca ccgctttatg tatacgggga aaattataaa ataaactatc
     4801 tgcatcctga tgagatgtgg ggagaagtat ttgaattttt taatgaaata ggaaagatcc
     4861 ctcaattaac cactccgctt ttagggcaac atcaagctga aaatgcagga gtagccatcg
     4921 aattgtattt tgtttactgt caattggaaa agctgccttt ccgagaaaaa gatgttttga
     4981 agggattgaa gaaatctcga tggccaggaa gaatggagcg tttgagtaac gagccattag
     5041 ttgttttaga tggtgcccat aatacgcatg caatgaaccg gcttgttgag aatttgaaaa
     5101 aagaattcaa aggatatcaa atcaacattt tgttttccgc cttacgaact aaaaatgtta
     5161 gagaaatgct agaacgattg cagcaagtac caaatgtcca tatttatttg acgacctttg
     5221 aacatccaaa agccatcgaa ttaagtgaag tatcagattt agcaagtgag agagtatcaa
     5281 tcgtttcatt gtggcaattc ggattagcag atatattgga aaagatgaca aatgaagacg
     5341 tattagttgt gaccggttca ctttatttca tttcagaagt tcgccattta ttaatacaat
     5401 taggaggaga tcatgaagta taatgcagtg atttttgaca tggatggttt gctgttcgac
     5461 acagagttag tgtattacga agccacacaa gtagtagcag acaagatggg ttttccttat
     5521 gataaagacc tgtatttaaa attcttaggt gtttcagatg aagaagtatg ggcgaattat
     5581 catatgattt tctcggaatt tggcaaaaca agtgtacaaa gattcataga tgattcatac
     5641 caggaaacgc ttgaacgttt ttcgcaagga gcggtacaaa tgaagcccgg tgtgttggaa
     5701 ctcctctctt ttttagaaga acatcagatt cccaaagttg tcgcttccag taaccaacga
     5761 gcagtcattg aacttttatt gaagaagaac aatctgtttg aacgatttga gacgattgtt
     5821 tctgcagaaa atgttcaacg agcaaaacca gacctagaaa ttttcttgtt agctaatcag
     5881 aagctaggta cagcgaagaa agaaacattg attttagaag attcacaaaa tggtatttta
     5941 gcagcttctg cggctgagat cccggtagtc atggtaccag atctgcttcc accatcccaa
     6001 gaattgatca aaaaaacagc tggggttgtt tcttctttac atgaaatccc tgcattgtta
     6061 aaataaaaat ccatctcttt tcgtatctta ttttagataa gatagaaagg agatgttttt
     6121 ttgggaaaac gattgatcaa agaagtacct actagctctt taccaaggga acgtatggaa
     6181 atttatggcg cggaagcttt atcggatcag gagcttttgg caatcttact gcgtaccggt
     6241 caacaccctt atagtgtgat gtcgattgct ggaaatctgt taaaaacgtt tggaggactg
     6301 gcctctttac gacaggctac tttgcatgag ttagaagaga ttcgagggat aggacgcgtg
     6361 aaagcaatcg aaatcaaagc cttgatcgaa ttaggtcgac gaattcagac ggatcatgaa
     6421 agagcggctc caccggttcg taccagctac gaattggccc agcaattgat tctggaaatg
     6481 aaagatcaca aacaagaaca tttggtttgt atttatttgg acacgaagaa tcaagtgatc
     6541 ttaaaaaaaa cagtgttcat cggttcgctg aatcagtcag tcgctcatcc tagagaaatt
     6601 ttccattatg ctgtacgtta ttgtgcagca aggatcgttt tagcgcataa tcatccttct
     6661 ggtaatgttt taaccatagt ataataaata atagtagtta aagtagttta agaggagata
     6721 ttaaagggat attttacttt aaataaaaat taacaaggtt tataatttgt agtagttaaa
     6781 gtatttaaag aggtgggaac atgtgaaggt tcaaaggata gaagtggaga ataagccgta
     6841 tccattgtat ttattactag ataaagaata ccagctaata gaaccagtaa tgaaatttat
     6901 taaatactta gataatactg gtaagtctcc taataccatt aaagcatact gctatcattt
     6961 aaagttgctg tacgagttca tggaacagag aggtgttatt cttaatgata ttaactttga
     7021 gttgttagca gacttcgtag gttggttgag atatccttca gcatcaaatg taattgatct
     7081 tcagtcaaaa aaagccataa gagaagaaac gacagtgaat acaattttaa atgtagttat
     7141 gagttttctt gattatttaa gtagattagg agaatttaaa tcaattgatg tatttaaaca
     7201 agcaaaggga agaaatttca aaggattttt acatcatgtt aataagggta gataccaaaa
     7261 gaatgtctta aagttaaggg ttaaaaagaa acagataaga acattgagat cagaggaagt
     7321 taagcaaatt attgatgctt gtcatacgaa aagagataaa ttaattttaa tgcttatgta
     7381 tgagggtggt ttaagaatcg gtgaagtgtt atcgcttagg cttgaagata ttgtcacttg
     7441 ggacaatcaa atccatttaa cacctagaga tgttaatgtt aatgaagctt atattaaatt
     7501 aaggaaggaa agaacaatac atgtgagtaa agaacttatg tcactttata cagattactt
     7561 gatatatgag tatagtgagg aattggagca tgattatgtt tttatttcct taaaagaagg
     7621 ctattttggg aaaccactaa agtaccaaag tgttcttgat ctagttagaa gaatagttaa
     7681 aaggactgga atagaattta catcacatat gcttcgccac actcacgcaa cgcagctaat
     7741 tagggaagga tgggatgttg cgttcgttca aaagagatta ggtcacgcac atgttcagac
     7801 aacgttaaat acctatgttc atctttcaga tcaggatatg aaaaatgagt ttaataaata
     7861 cctcgagaga aaggagcata agaaatgaat gcttctagta aaaggaaaat tattagtcag
     7921 agtgagatta gcaaaaaaat agctgtaatg aatgaagaaa tgcaggggtt ttgggctaat
     7981 aatagttggg atataagaaa atgtccacat ccttctgcca tagaattaag taagaatcct
     8041 gctttaagga atcgttgggt tcgttttgaa cgtgttaaaa atctgtggtt aagaacagaa
     8101 ttgaaatatt tttattttta ccatttaaac aatggaatat ggaatgcaaa aactgtctgg
     8161 attagaaaag gaacagtaat taataaaatg ttagatttct tagatttaaa gtatcctagc
     8221 attacttcaa ttactgaagt tcctattgaa aaagcaatga cggagtatag aacttatttg
     8281 acaaaacggg gtgttagaat taccactact aattataaga ttactgctaa tcaagaaaaa
     8341 acacctgtaa aagctaattc ctactatgtt actaatctaa aacaatttat ggagttttat
     8401 gagaactttt attttgatgg agaggagtgg gataaagacg tttgggatag acgtaactta
     8461 cctttgccag atgataaggt taacccaaca caatatgaat atacaattaa ctttaaaggg
     8521 tttcggaata catattttaa acaacttgta aaaagatatt gtaagttgag attgaacatg
     8581 gatagctttt cctatgtaag tgatattgcc caaagactta aagagttctt taattttctg
     8641 gacatgaaat ttaaacaagt tcagagagta caccaattaa cgagagtgga aattgaagca
     8701 tatttaagtg aactaaacat gatgggaata aaacctagta caataactgg gaggatctct
     8761 atattggaag gactatttag tacccttctt aggctagaat gggatgatgt tccttccaaa
     8821 atattaattt attctgagga ctatccgaaa ataccaagag caaaaccacg ctttatagat
     8881 gaattcgtcc tagagcaatt gaacagtcat cttgataaat tacccgaata tatagctacg
     8941 atgactatga ttgttcaaga atgtggaatg aggataagtg aattgtgcac cttgaaaaaa
     9001 ggctgtctat tagaggacaa agatggagat ttctttttaa agtattatca atggaaaatg
     9061 aaaaaggagc atatagttcc aatatctaaa gaggtagctt tacttattaa agttcgggaa
     9121 gataaagttt cagaggaatt tccagatagt gaatacctct ttccaagaaa agatggatcg
     9181 ccattaaaac aagaaacatt tagaggtgag ttaaataaat tagcttatga gcaaaatata
     9241 gtggataaat caggtgagat ttatagattc catgcccatg cctttcgcca tacagtagga
     9301 acaagaatga ttaacaacgg gatgccccag catattgtgc agaaattttt ggggcatgaa
     9361 agcccagaaa tgacaagcag atacgctcat atctttgatg aaactctaaa aaatgaattt
     9421 actaaatttc aggaaaaact ggttaccaat aatggagatg tgcttgatct agatgaagat
     9481 aatgaagtcg atgatgtaga gcttcaatgg ttcaagaaaa atataaatgc acaagtgctt
     9541 ccaaatggtt attgtagatt gccagtagta gcaggtggtt gtccacatgc gaatgcatgc
     9601 ttagattgca ctcacttctg taccagtaag caattcttac cacagcacga agaacagtta
     9661 gagcgtacag aagagttatt agccatagct aaggataaac aatggcaaag acaagtagag
     9721 actaatagcc gtgttaaaga gcgtttagaa caaatcattg gaagtttgac ggggtaatta
     9781 tcaatggata aacaagttag aaatacaaca gaaattgtac gtttggcgaa gcagaaatca
     9841 aaaaagacaa gggaaaaagt agacaaagcg atttctaaat tttcgattga aggtaaagtt
     9901 attaatttta attcaatagc aaaggaagct aatgtttcta aatcatggct ttataaggaa
     9961 cacgatatta ggcaaagaat cgaatccctt cgtgagcgtc aaataacagc aaatgtagtc
    10021 tcaaaaccca agaaaagttc tcgttcggag gaaatcctta ttaaaacctt aaaaagaaga
    10081 gtaatggaat tagaaaaaga aaataaaaaa ttacagaacc aaattcaaaa attatatgga
    10141 gatctgtata ataaagaata attattaatc tgtagacaaa ttgtgaaagg atgtacttaa
    10201 acgctaacgg tcagctttat tgaacagtca tttaggtata tgtccaatct agggtaagta
    10261 aattgagtat caatataaac tttatatgaa cataatcagt tacgaggtga aatcatgagc
    10321 aatttgatta acggaaaaat accaaatcaa gcgattcaaa cattaaaaat cgtaaaagat
    10381 ttatttggaa gttcaatagt tggagtatat ctatttggtt cagcagtaaa tggtggttta
    10441 cgcattaaca gcgatgtaga tgttctagtc gtcgtgaatc atagtttacc tcaattaact
    10501 cgaaaaaaac taacagaaag actaatgact atatcaggaa agattggaaa tacggattct
    10561 gttagaccac ttgaagttac ggttataaat aggagtgaag ttgtcccttg gcaatatcct
    10621 ccaaaaagag aatttatata cggtgagtgg ctcaggggtg aatttgagaa tggacaaatt
    10681 caggaaccaa gctatgatcc tgatttggct attgttttag cacaagcaag aaagaatagt
    10741 atttctctat ttggtcctga ttcttcaagt atacttgtct ccgtaccttt gacagatatt
    10801 cgaagagcaa ttaaggattc tttgccagaa ctaattgagg ggataaaagg tgatgagcgt
    10861 aatgtaattt taaccctagc tcgaatgtgg caaacagtga ctactggtga aattacctcg
    10921 aaagatgtcg ctgcagaatg ggctatacct cttttaccta aagagcatgt aactttactg
    10981 gatatagcta gaaaaggcta tcggggagag tgtgatgata agtgggaagg actatattca
    11041 aaggtgaaag cactcgttaa gtatatgaaa aattctatag aaacttctct caattaggct
    11101 aattttattg caataacagg tgcttacttt taaaactact gatttattga taaatattga
    11161 acaatttttg ggaagaataa agcgtcctct tgtgaaatta gagaacgctt tattacttta
    11221 atttagtgaa acaatttgta actattgaaa atagaaagaa attgttcctt cgatagttta
    11281 ttaatattag tgacatttgc atgcttcaaa gcctgtcgga attggttttt agtgaaaaga
    11341 acacgatatt cacggtttac ccacttataa acaaaagatc gatacttttt gtagtccttc
    11401 tttgaaatca atggttgatg tcgttcaaga acaatcaata cagagtctac acttggctta
    11461 ggatgaaaat atagtggtgg tacttttttg agcattttta tatccatctc caccattaat
    11521 agtaaaccca aagctcgttg cagattttgc aatcttttcg caaatccctt ctcaacgata
    11581 agatagctat atttagcctg actttcaaag gtaattcttt tgacaatatc cgtactgatg
    11641 ttataaggaa tattaccata tatcttatag tttatatgtt ttgggaagga aaattttaga
    11701 atatccgttt gaatcacttt tatattctca gaggggttta ccgcttcttt agtcacttga
    11761 cataagcctc catcaatttc tatagcagta actgatcgac tcattttgac tagctctttg
    11821 gtaaaatgtc cttttcctga tccgatttct attacgttgt cttgtttact gatattcgtg
    11881 tgattcaata tttcttttac atgcttttta gaagtaataa aattttgcgt gtctttaggg
    11941 tttttctggt tcattataac cttctcctta ctggttataa tgaactgaca ttaagcgttc
    12001 attataacca attaattttg atttggttga taacgaactt tattaataac aaatatagaa
    12061 aaagtaccca tacttttatc tcctttccat ttttttcatt atgaatgaga taaagtaatt
    12121 tctactactg cgatactggt gcacataatt gaaacctcct tatgtaactg aatatgattg
    12181 tatcatcttt tcattttaga ataaaccttt atatgtaatt ttgcacttga cacttcagat
    12241 ggacgttttc ttcaaaataa tatgttttga aacttgataa gagaagccac tgtctttact
    12301 aaaaagtaaa ggctgcgata aaggacagtg actttaataa agttgcactg ttgtattgtc
    12361 tttggataaa attgtctaaa gggaagtttt atccccaacg aaaaaacatc taaagtggta
    12421 agtaatgaaa gaatgtcgat aaagtaggaa tgggggatct ttttgaatac aatagaatca
    12481 cagtttgata aggttgcaga agattacgat ttcgtgaatg agcttttgaa tgattattca
    12541 ttctttgtgt ctaatatgtc tccaaagaaa ggcagagcat tagatatcgg atgtggctcg
    12601 ggtttgttag tggagaaatt agcaagttat tatgatgaag tggtagggat tgatatttct
    12661 aatcaaatgc tcgatcttgc caaatctaaa cgtcaactaa caaatacggt ctatctgaat
    12721 atgaatgcag aacaacttaa ttttaatgag aagtttgatt ttattgtaag ccgaacaacc
    12781 tttcatcatt tggatgatat agccagcgta atacagcaaa tgaaggaact gttgaatgag
    12841 gaaggaagaa tagttattct tgataatgta tctgaagtcg agacaccgcc tacctatgtt
    12901 tataaattag gggcaatcca agagttttta ccgcattgtt tcaaatttgg aataaaaaat
    12961 gcgattagaa tttataatca caacacatct aaatcatggc ttgagcattt agcttcagat
    13021 aagtacctat ctgaacaaaa ttattacgat ttatatgaaa agttgttgcc tggatgccaa
    13081 ttccataaaa tgggttgggc aatgggggtt gtctggacaa aataacttaa agctgtggct
    13141 ttgaaaagtt gaatttgtga ttgttttcag agctatcggg tgctttagta taaataacaa
    13201 tgaataaaaa atcagcctgt atcttatgtt actattaagt aatttaagat acaggtttta
    13261 ctatttttga aataagtacc ttccacttca aatacatgta ttcaaagtga aagtattgat
    13321 ttaaatgatt cttttgtaat ttactcttga taatatgaaa ttccatcgca gcaggatatg
    13381 aattttacca aaaggataca aaagtgtgga gaaatgatgg ggatcactgt actggaccac
    13441 ttgatcatcg gacgcaaacg atattttagt ttgagagaag aaggaatgat ggaagagaag
    13501 taagttgctt tcttgcaaaa aaaatgaagc aagtgtacaa ttatattgta attttgtttg
    13561 ggaatagaag ttgaaacatc tgttgcacct ctatcgtaca cgagcaatgt tacaatttaa
    13621 attagtgctt ttggcacgag gaggatatta ttatggaaaa cggaacagta aaatggttca
    13681 acgctgaaaa aggatttgga tttatctcac gtgaagatgg aagcgacgta ttcgttcact
    13741 tctcagctat ccaaggtgac ggattcaaaa cattagaaga aggtcaagca gtgactttcg
    13801 acgttgaaga atcagaccgc ggcccacaag ctgcaaacgt tgtaaaagct taattttatc
    13861 tgtaaatcaa attgatggta gacatcaatt tttacgagag aacaaagcga gtaacaaaaa
    13921 tacctgccac aagagacaat ttctcttgtg gcaggtattt ttttgtatga aaaagtaaat
    13981 aaatttaaga ggctatgaaa gagccacctt gtatcaagga ataagaacaa acaaaaatgg
    14041 cttctcatgt ttttcatttg ttttctctta tattcaggac aaggtgctga tttcataacc
    14101 tcttatttat tcttcatctg gtgtcattac cattggcaaa atcattggat gtctttctgt
    14161 acgttcgaat aagaaaggct gaagcgcgcc agtcatcgct tctttcaatt tatattctga
    14221 acagtcaggt ccattcaaac attctcttaa agcgtggaat aagattcgct gagcttcatt
    14281 gatcatttct cctgattctc gcatgtagac aaatccacga gagagaatat ctggaccagc
    14341 caagatttct tttttcttga tatccactgt cgcgacagct aatacaagac cttcttctga
    14401 aagcaggtga cggtcacgta aaacgacatt accaatatca ccgatcccgc taccatcgat
    14461 atacacatct ccagccacaa aatgcccagc aggacgcgca gaatcgctag ttaacgctaa
    14521 aacatcgcca ttttccatga tgaagcagtt ttctgcagga acacctacat cttgtgctag
    14581 agaagcatgt actttcaaca tgcggaattc tccgtggaca ggaataaaat attttggctt
    14641 catcaaacga agcattaatt tttgttcttc ttgtccaccg tgaccggaag tatggatgtt
    14701 attgattttt ccgtgaatca cttctgcacc agcttcagac agcaaattga tcaaatgatt
    14761 gacacttgtt gtattaccag gaataggtga acttgaaaag atcactgtat cgtctggctg
    14821 gatttggatt tgacggtgag taccattcgc gatgcggctc aacgcggcca tcgcttctcc
    14881 ttgagaacct gtacatagga tcatcgtttc attggcaggc aagcgattga tttcgtgtgc
    14941 atctacaaat gtgccgtctg gaactttgat atatcctaaa cgttgaccgt tgacgatggc
    15001 attttccatg cttcgtccga aaacagcgat tttacgacca gttgccacag cggcatctgc
    15061 tgcttgctgc aaacggaaga tgttagaagc aaagctggca aaaatgatac gtccttcgat
    15121 ttttgaaaag atcttcaaaa tagaatgccc aatcgttttt tctgatttag taaatgttgg
    15181 aatctcagca tttgtactat cagataaaag gcacaagact ccttcttccc cgatacgagc
    15241 cattttatgc aagtttgctg gttcgccaac aggagtgaaa tcgaatttga agtctccagt
    15301 cgctacgata tttccagaag gtgttttcac cacgatccct aatgcatctg ggatactatg
    15361 ggtcgttctg aaaaagctga cagaagtctt tctaaaacgg atgatcgtat cttcattgat
    15421 ctcatggagc tctgcatctc gaagcaagcc atgctcatct aatttgtttg tgatcaacgc
    15481 taatgcgaga ggaccagcat agataggaat atttgcttgt ttcagcaagt agggtattcc
    15541 gccgatgtgg tcttcgtgcc cgtgagtaat caccaaggct tttactttgt gtaagttttg
    15601 tatgatgtag ctgtagtcgg gaatgacata gtcaattcca agtaaatcgt cttccgggaa
    15661 tttgatcccg gcatcaatca aaatgatttc atcttggaat tgtaccccat acgtattttt
    15721 cccgatttca cctaatccac caatagcaaa gacgcctgtc tcgttatttt tgatggaaag
    15781 tttcatatta aaactccact aatgagaaat cagcattttc tttttcgtac tctagatgac
    15841 ttccttctag aagttgaacg aattcaatat tgtatggggt attttcttcc acacgttggc
    15901 gagcgtctac atcgcttttt gcctcaatgt aaagagattt tgtttcttcc cgtttagggt
    15961 tgcgtacttt tgtttcttga taataaactt tatagatcat tgttttgtct cctttatctg
    16021 cgtaaagcag tgatttagct agttgatttt tttagacaac acaaaacgag gggacaagcc
    16081 acctggtttt tcatatgctt tgactagaat actggattcg agtcaaagag acaagcctcg
    16141 tttttgctta tctgttcgga tattttttca accgtacctt ctgtataaaa aatatacaac
    16201 aattaattgt tattctagca taaaacagca aataagtata gaaatcaact agattgaaag
    16261 aaaaaatcaa tgtttatcat cccgctataa tcataatgac ttttttatga gagaagtgtt
    16321 ctattaatag gctcgttgtg ctatcatttt tagtaatgaa aatgtaacaa aagattaaaa
    16381 ggagatgaga aagtgaaact aatgtggcat tataccatgc gttataagaa attcttgttt
    16441 ttcaatttca tatgcgtatt tggttttatt ttgattgaat taggacttcc tacgatttta
    16501 gcacgtatga tcgatgtagg aatccgcaat gatgattttg attatgtgaa gcaacaaggg
    16561 atatttatga tcgttattac tgtaatcggt attattttga atatcttgtt aggttatttt
    16621 ggttcgagga ttacgactaa tatcgtagca gatattcgag atgatttgtt cgaaaagatc
    16681 caaagttatt cgcatcagga atacgaaaat ctaggcgtat cttcattgat cactcgtaca
    16741 acaaacgatg cttatcagat catgttgttc ctgcaaaata ttttacgtat cggttttatg
    16801 gcaccaatga tgttcattgt cagtttgtac atggtcatgc gtacaagtgt gacactcagt
    16861 ttgtatgtag taggtgcatt acctttacta ttattggcag tcgtagcgat tgctaaggtg
    16921 tctgaaccgt tatctaaaaa gcaacaaaaa aacttagata aaatcaacag tatcttgcgg
    16981 gaaaatcttt caggattgcg tgtgattcgt gcattcgtca atgaaaagtt tgaagaaaaa
    17041 cgttttgctg tagtcaatga gaattatgct tctagttcaa aaagcttatt ccgtttgatg
    17101 gctgttgcac aaccgggttt ttatttctta tttaatatcg taatggtatt gatcatctgg
    17161 agcggggcag tgcaaatcga tgcaggcgag cttcgtgtag gagatttgat tgcgtttatc
    17221 gagtatatat tccatgcctt gttctcattc atgttatttg ctagtgtgtt catgatgtat
    17281 ccaagagcag ctgtttcagc tggacgtatc caagaagccc tagatgccaa accttcgatt
    17341 gtagaagatc caaatggtat atctgataca aaaactaaag ggtatatcga atttcaaaat
    17401 gtaacatttg cttatccagg ccattcgcaa gagccagtga tacgaaatgt cagttttaca
    17461 gcctctccag gagagaccgt tgcttttatt ggaagtaccg gtagcggaaa atctacgttg
    17521 atccaattga tccctcgctt ttatgatgtg agtgaaggaa agatcttgat cgatggagta
    17581 gacgtgagag attatcggct aagcaagttg cgagataaga ttgggtttat tccgcaaaaa
    17641 gcgctgctat ttactggaac gattgcagat aatttgcgat acgggaaaga agatgctacg
    17701 caagaagaaa tggaacgtgc agctgagatt gctcaagctt cagaattcat ttctcgaaaa
    17761 ccagatggat ttaatgaaca tttatcagaa ggtggttcca atttttcagg tggacaaaaa
    17821 caacgattgg caatagccag agccatcata cgccgtccgg aaatctatat ttttgatgac
    17881 agcttttctg cattagatta tcaaacggat gcgaagttac gtgctcgtct gaaaaaagaa
    17941 acgacagaat ctactgtatt gatcgtagca caacgtgtgg gaacgatcat gcatgcagat
    18001 aagatcatcg tattaaatga aggagaagtc gtcggtatgg ggacgcatcg ggagttgttg
    18061 gaaaaatgcc cgatttatta tgatattgcc gcttctcaat tatcaaagga ggaattagca
    18121 tgaaaaaaac cttttcttcc ttaaaacgtt tagctcgata tatcagaccg tatcgtgaga
    18181 cttttctttt agtgatccta tttacagttc tgacagtggc tttcaacgtg gctttgccat
    18241 atgttacagg acttcctact acagaaatca gccgtaatat tgccaatggc gaatcaatca
    18301 atttttctta tatattcaaa tgtctcattt ggatcacagt tgttggaata gggtattgta
    18361 tttcgcaatt actttctggt gttttgatga caaatgtcgt tcaaagtgcc atgcatgatt
    18421 tgagaagaga catagaagaa aaaatcaatc gtcttccggt ttcatatttc gataaaaacc
    18481 agcaaggaaa tattttgtct cgtgtgacga atgatgtaga tgcagtcagc ggtgctttac
    18541 aacaagcttt tattggtatc gtaaatgcga tattgggaat tacgttggct gcagcaatga
    18601 tgttttatat ccagccgatg atggcgctga tctctatgat catgattcca acatcgatct
    18661 ggatctcaaa acgagtgatc aatgcttcgc agaaatattt tcaaagcatg cagaattctc
    18721 ttggtgagct aaacggctac gtccaagaaa atatgacagg ttttagtgta ttgaaagtat
    18781 atggacgcga aaaagaaaca ttggaaggat tcaaaaaagt taatcacagc ttgaaatact
    18841 ttgggtttcg tgcagcattc atttctggct tgatgatgcc tctagttcaa ttaactgcct
    18901 atgctactta tattggtatg gctgttctag gaagctttta tgttattact ggggcgatcg
    18961 ttgtcggtca actacaagca ttcatccaat atatttggca aatcagtcag ccaatgggga
    19021 acgtgacaca attgtcttca gttttgcaaa gtgcatcagc ggcaacgaaa cgtgtattcg
    19081 agattttaga tgagccagaa gaaaaagaaa acaaagtaga tgcttcactg ccagaaacga
    19141 ttttaggaga tgtgacattc gatcatgtcg attttgcata tgatccgaag aaaccattaa
    19201 tccaagattt gaattttgaa gtgaaagctg gtcaaaccgt agctgtggtc ggtccgaccg
    19261 gtgccggcaa gacgacattg atcaatctat tgatgcgttt ttatgacgta gacaaaggag
    19321 caataaggat tgatggaatc gacaccaaat caatgagtcg cagtgatgtg cgttcgctat
    19381 ttggtatggt cttgcaagat gcatggcttt atcaaggaac gattgcagat aatatccgct
    19441 tcggaaagct tgatgcgaca gattatgaag tagttgatgc tgcgaaaaca gcaaacgtag
    19501 atcattttat ccgaacgatg ccagatggat atgatatggc catcaattct gagggagaca
    19561 atgtgtcttt aggacaaaaa cagttgctga cgattgcccg agcagtggtc tccgatccta
    19621 aaatcttgat tttagatgaa gcaacaagtt cagtagacac ccgtttagaa gcgttgattc
    19681 aaaaagcaat ggatcgcgtg atggagggac ggacaagttt cgtcatcgct caccgtttat
    19741 cgacaatccg tgatgcagat ctgattctag tgatggatca aggtcagatc attgaaaaag
    19801 gaacgcatga gagtttattg gcacaaggtg gattttatga aaaactgtat aacagccaat
    19861 ttgctgagga agcagaataa gtcatataag aataaaacag tttaaaactt attttttgtt
    19921 ctctattctt gataatggta aagtaaagtt atcaagaaaa gtgaggacac ggagatggaa
    19981 cagtttttaa atgaaaaaat tactgaagcc aaaagagaag ttgatcgttt aacgcacgtc
    20041 tgtcagacga agccttttga ttcagctgat ttttttgaaa atgagtttca aatcgaacgc
    20101 ttggcagcac aaatagaagt gtacgaatta attttaaaaa aagtaataga agaaaatata
    20161 gatgtacaat aaaaacaggt aaaacgtcag ttaccagata ctgtaaatag acatctggcc
    20221 tgagcaggtt ttacctgttt tttttcttat gaaataaata tatgctatga tacctttatt
    20281 caataggatt agtttctaca aaaaatgaaa ggagaaaaat aaatatggaa cggtttatag
    20341 catcaaatgc cacagtgatt ggtgatgtga cattgtcaga agatgtgacg atctggtatc
    20401 aagctgtttt acgcggggac agtaattgga taaaaatagg acaaagaacg aatatccaag
    20461 atggtacaat catccatgtc gatcatgatg cacctgtaga tattgcggag aatgtgacgg
    20521 taggacatca gtgcatgcta catggctgta caatcgaaaa aggggcgctg atcgggatgg
    20581 ggaccacgat attgaatcat gcggttattg gtgagaatag tttgattggt gctggatcat
    20641 tagtaacaga aggaaaagtt attcctccta atgtcttggc ttttggtcgc ccagctaaag
    20701 tgatccgtcc tttgacaaaa gaagaaatac aaaaaaacaa agaaaacatc cagcattatg
    20761 tcgagatagg tcggatgcat gcgcaaggaa aattcaagga gcaaagatag gcagtagcat
    20821 ccataaaata aaaaaggatg gtcagtttga gttgaccatc cttttttatt tggtttagat
    20881 aagagagctt aaaaaagctt cctttgaaac agattaattt aatgaagaac tgttttccat
    20941 gacttcatcg atcaatccat atgcttttgc ttgttcagct gtcatatagt tgtcacgatc
    21001 tgtatctttt tcaatcactt caagcggttg tccagtgcgt tcagctaaaa ttttgtttaa
    21061 gcgttcgcgt gtttgcaaaa tatggcgtgc cgcaatttcg atttcagttg cttgcccttg
    21121 agcgccgcca agaggttgat ggatcatgat ctcagcattt ggtaaagcaa atcgtttacc
    21181 ttttgtacca gctgtcagca agaagcttcc cattgaagca gccataccca taacaattgt
    21241 ttggacatct gctttgacaa agttcattgt gtcgtaaata gccatacctg ctgtaactga
    21301 accaccagga gaattgatat aaagatagat atctttttca gaatcttgag cgtctaagaa
    21361 aagcaattgg gcgatgatcg aattagcaag atcatctgtt acttggccgc ttaacatgat
    21421 gatacggtct ttcaataaac gtgagtagat atcgtaagct ctttcacctc gtgaagattg
    21481 ttcaatgact gtaggaatta aattcataat agagttcctc cttgatttcg tttagaaata
    21541 gtgtaccata atttagtttc taatacctaa aatgtagtat acatttttgg tcaaaaaagg
    21601 tcaaataaaa aacttaggca aattagtcgc ataaacccgc tttatggtat gataagcaga
    21661 gaaacaaaac aaggaggaac aacagattga tagttggtat tactggcctt atcggagtgc
    21721 tgacaggtgc tgtagttgtc tatatcggca tgatactgca aatgcgtcac ttggaaaata
    21781 aagaacagcg tagacgtgaa ttggaattga aagtcaaaga aatcgagacg ctgaatcaat
    21841 taaataaaaa gatcaacgag atcttgcaaa aacggacaac gctgatggaa gaatatgtca
    21901 gctttgatgc atttgatgat tgttatatca cgattgacga ttttgcgtat ctccaatcat
    21961 tttcagcgca aaatagtttt tatctgccaa attatatttt agacgagcta tttaagaata
    22021 tctcccatcg aaaagtgatt ttatctccag atgaaacaat gaagattggt ggatatgctt
    22081 ataaaggcgg acgcgtgatt ttagagaatt ttacggatag tctgactgaa atgatcaatg
    22141 aaaaaaaagc tcagttgaag actatgacgc gccagccgat tggtttcttc tcgaaagatg
    22201 atgtttggag ctgatatttt gaagcattgc cgttgcacat gagagcgtgg taatgctttc
    22261 tttttttctt aatgatacaa tagaactgaa cggagtgaag aaagatgtta caaatctatg
    22321 cccatcgcgg aagcagcggc acccacccag aaaatacgct tcctgcattt gcagaagctg
    22381 tacgagtagg tgcagatggt atcgaactag atgtccatct aagcaaagat ggttatttga
    22441 tcgttatgca cgacgaagaa gtggaccgga cgactaacgg taaagggctg attcgagaaa
    22501 aaacattgga agaactcaaa aaattgaatg cagggggcaa attcagcaaa gaatttccag
    22561 cagctaaagt tcctgaattg agagaagtgt taggactgct cctccaaaaa aattaccgag
    22621 ggatgctaat gattgaaatt aaaactgatc agtatgaata tcccggtatc gaagaaaaat
    22681 tagccgaaac attaaaaagt agagattggc cttttcaaca tgcatattgc agctttcatt
    22741 tggattcttt ggaaaaaatc gcattactgg aaccggagat acagctggac ttattgatga
    22801 gtacttctga taaaaagatc caactagcag cagaaacccc ttatatagaa ggagtacatc
    22861 caaaaaatac ttggttgttc gaacatccag tccaggcagc tgattttcca aaagcaattc
    22921 gtgcatggac gattaacgat gaagaagaaa tggccaaatg tcaagaactc aaaatagatg
    22981 gaataataac tgattttccg gagaaagctc ttcggtggaa acaatcaacg aaaagtaggt
    23041 aaaatatgga aaaagtatta gtgatcgtcg gtccgacagc agtcggtaaa acagcgctaa
    23101 gtgtggaact cgcacaacta tttggaggag agatcatcag tggtgattca ttgcaaatct
    23161 ataaaaatct ggatatcggt acagcaaaaa tcaaagaaga agagatgaat aatgttccgc
    23221 atcatttgat tgatgtgatc gaacctactc aacgttactc agccgctgat tttcagcatt
    23281 caggtcgaca attgattacc gagattgcga accgagggca cctacctatc atagctggtg
    23341 gtacgggtct ctatatccaa tcccttctat atgattatca gttaggagcg acagaagaag
    23401 cgaatcctga gattagaaaa aagtatgaaa cgttagctga aacaattggt tcccaagcat
    23461 tatggaatca gttgaaagaa aaagacccgg cagctgctga gaaaatccac tggaacaatc
    23521 aacgcaaagt gataagagct ctggaagttt tagagacgac aggacatagt atcacggcac
    23581 ctaaagaaga gccaaagcaa ttgtatgatt acctcatgat tggattagat acgaagcgta
    23641 gtattcttta tgaacgaatc aatcaaagag tcgatttgat gctagcagaa ggtttagtag
    23701 cagaagcgcg taaggtgtat gagcttggag aagtccaggc cagtcaaggt attggatata
    23761 aagaattttt cccttatttt gaagggaaag aaacattgga agaagcaaca gaacaagtca
    23821 aactacattc gagaagatac gcgaaacgtc agttgacgtg gtttcgtaac cgtttggatg
    23881 cacagtggtt tgatcttata agtgagccaa atcaaaaaag tcagattgag caaacaatca
    23941 aaaaatggct ggaggcggaa taaaatggaa gaaaaagtga tcatcgtagg ggtagaaacc
    24001 gaagcaaacc agcgttactt ttcagagtca atggaagagc tagttcagct gacgaatacc
    24061 gcatcaggag aagtcgtatt cacaattaca caaaagagac cacaagtgga tcggcaaacg
    24121 attatcggta aagggaaatt acaagaatta gttcaacaag cggatgcgca tgaagcggat
    24181 ttgatcatct ttaatcatga gctaactccg agacagagcc aactgattac cgatgctgtt
    24241 ggtcttccgg taattgatcg cgtgcagctt atcttagata tttttgcgat gcgtgctcgt
    24301 tcgaaagaag ggaagctgca ggttgaactt gctcagttag aatacctgct gcctcgttta
    24361 gtagggcaag ggaaaacatt atctcgtctt ggcggtggga tcggaacaag agggccaggt
    24421 gaaacaaagt tagaaacaga ccgccgacat atacggaata aaatttttgc tgttaagaaa
    24481 gaattgaaag aagtagaagc tcatcgagag cgcaatcgcc aaaaaagaaa aaatagcgag
    24541 atttttcaga ttggacttat tggctacacg aatgccggga agtcaactat cttgaatcta
    24601 ttgacacaag cggatactta ttcgaaagat caattgtttg caacgttaga tccattgact
    24661 aagagatggc gttttgctga aggatttgag attactgtaa cggataccgt cgggttcatc
    24721 caagacctgc ctacgcaact gatcgatgct ttccattcta cattagaaga aagtcaaaat
    24781 atggatcttt tgctacatgt agtcgatgct agttctccag atcgtatttt acaggagcag
    24841 actgtcttaa aattgatgga tgaactgaat atgaaagaaa tgccgatact gactgtttac
    24901 aataaggcag accagatcga cccagcgatg ttcacgccta cgctattccc aaatgtattg
    24961 atttctactc agacgaaaga aggtaagatg gcgttgatcg aagcaatcaa acgtcagctg
    25021 atggaactaa tgacccctta tacactttct gtgccaagca acgaaggaca gaaattaagc
    25081 gagttgagaa gacaaacaat ggttttggac gaagcgtaca tggaagaaac aaacgaatat
    25141 gaagttaaag ggtttgcaaa aaccaattca aaatggttaa gaaaaaacca ataaaatttt
    25201 atttttttaa
//
LOCUS       DACEEZ010000033        24581 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20313_2017
            SAMN12639966-rid9879223.denovo.033, whole genome shotgun sequence.
ACCESSION   DACEEZ010000033 DACEEZ010000000
VERSION     DACEEZ010000033.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639966
            Sequence Read Archive: SRR10023158
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 24581)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24581)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741683.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741683.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:44:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,016
            CDSs (total)                      :: 2,962
            Genes (coding)                    :: 2,889
            CDSs (with protein)               :: 2,889
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 36 of 73
            Pseudo Genes (incomplete)         :: 29 of 73
            Pseudo Genes (internal stop)      :: 18 of 73
            Pseudo Genes (multiple problems)  :: 8 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24581
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639966-rid9879223.denovo.033"
                     /isolate="E20313_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(<1..686)
                     /locus_tag="IWO89_001399"
     CDS             complement(<1..686)
                     /locus_tag="IWO89_001399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAQ0456159.1"
                     /translation="MNILMIEDNQSVSEMMQMFFLNEGWEATFKYDGKEGLDAFLENP
                     QHWDMITLDLNLPTLDGMLVGREIRKVSSTVPIIMLTARDSESDQVIGLEMGADDYVT
                     KPFSPLTLIARMKALHRRSELAEAREEAAVSDDHFDIETSHFKMNTKTREAYLDNRPI
                     EGLTPKEFDLLFTLAKKPRQVFSREQLLELVWDYQYFGDERTVDAHIKKLRQKIEKVG
                     PQVIQTVWGVG"
     gene            complement(667..1017)
                     /locus_tag="IWO89_001400"
     CDS             complement(667..1017)
                     /locus_tag="IWO89_001400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SdpI family protein"
                     /protein_id="HAQ0456160.1"
                     /translation="MIFFSIGILFLIIGSLFRILPSKGNLPFYGYHSPLAAKTDAHWR
                     LAQKTSGNWFFLMGLLMALIGYYLKTSGHTNYFLIEMLLLVFPIMPIFIMTEKKLQKY
                     DLETGGNDNEYFND"
     gene            1161..1934
                     /locus_tag="IWO89_001401"
     CDS             1161..1934
                     /locus_tag="IWO89_001401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HAQ0456161.1"
                     /translation="MSNYRAITFFDLDGTLLDAKSKITPEVAKAMNALKENNVLPVIA
                     TGRTEAEIHSIMEDAGITSAVTMNGSFIRVDGEEIYSQTFSSEECQKMYDHVQLNGHE
                     LSYYNDQKIWCTGHSQTMINAYDFIHSDVPEIDPLGFKENTVNMLLILSQSGDEYYHE
                     HFPELTFYRNGPYSIDTVKKGISKGAGVKRLQKELQLEKVPTFGFGDGPNDFALLEAC
                     DNKIAMGNAYDGLKELSTFITKKNTEGGIVHALKYFDLI"
     gene            2105..2471
                     /gene="ssrA"
                     /locus_tag="IWO89_001402"
     tmRNA           2105..2471
                     /gene="ssrA"
                     /locus_tag="IWO89_001402"
                     /product="transfer-messenger RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00023"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00023"
     gene            complement(2905..3219)
                     /locus_tag="IWO89_001403"
     CDS             complement(2905..3219)
                     /locus_tag="IWO89_001403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0456162.1"
                     /translation="MDFYSNFILIIAILLLLNIWFFDKSRNAGIGFRTKRSTSSEKKW
                     VYSQTIFYGGVISISLLSSTLYSFNVIDVSMSNFISIIGILISAIITQLLLVFEEKSK
                     NN"
     gene            3505..3744
                     /locus_tag="IWO89_001404"
     CDS             3505..3744
                     /locus_tag="IWO89_001404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0456163.1"
                     /translation="MKRILFISLSNLLLLTACGTSASKNIPSDTQISNSIDSTISTNE
                     KNSPVETSDIKVSTSAKNRVKKLSARLLYFKQTLN"
     gene            complement(4106..4366)
                     /locus_tag="IWO89_001405"
     CDS             complement(4106..4366)
                     /locus_tag="IWO89_001405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0456164.1"
                     /translation="MNNETIILGKKYICQPIGLKQKVVGEVISKLENCVVICVDEYHD
                     VDRNEILEKCGKVVAKYTHVYELETTEYLYNYSNKYEHTLVS"
     gene            complement(4551..5048)
                     /locus_tag="IWO89_001406"
     CDS             complement(4551..5048)
                     /locus_tag="IWO89_001406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HAQ0456165.1"
                     /translation="MWTEKQKKDAYEILLLQQQGLLEAEDNWLANLANSSALLNETLP
                     ETVFAGYYLFDGNELILGPFQGRVSCTRIKMGKGVCGESAEKQETLIVDDVKTHANYI
                     SCDSAAQSEIVVPMVKEGNLIGVLDIDCGVTKGYDEIDQAFLEKYVDLLLANFNINAF
                     SFINN"
     gene            complement(5178..5888)
                     /gene="budA"
                     /locus_tag="IWO89_001407"
     CDS             complement(5178..5888)
                     /gene="budA"
                     /locus_tag="IWO89_001407"
                     /EC_number="4.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate decarboxylase"
                     /protein_id="HAQ0456166.1"
                     /translation="MTEKILYQHGTLGALMAGLMDGTETIAHILEKGTLGLGTLHGLD
                     GEVIFLDGVAYQGRSDGSVAQLDGSELTPYAAITDFTPDTSFSVSKTADGEQLKKDIL
                     VREAGENLFLAVKITGLFKNMHIRIMPKQEKPYRRLAKISESQPEFQQSNIQGTLVGF
                     FTPELFQGVAAAGFHLHFIDDTHTFGGHVMDFEVAEGKVEISRISSLVQHFPVDDPTF
                     VHETIDYADLAAEIQQAE"
     gene            complement(5901..7565)
                     /gene="alsS"
                     /locus_tag="IWO89_001408"
     CDS             complement(5901..7565)
                     /gene="alsS"
                     /locus_tag="IWO89_001408"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase AlsS"
                     /protein_id="HAQ0456167.1"
                     /translation="MAEKMKQGSKSVVESLINHHVDYVFGIPGAKIDGVFNELEDQGP
                     ELIVTRHEQNAAFMAQAIGRITGEPGVVIATSGPGASNLATGLVTATAEGDPVLAIAG
                     QVKRSDLLKLTHQSMDNAALFQPITKYSAEIQDPETISEVIANAYRMAKSSKKGASFI
                     SIPQDVVDAPVQGNVIKPLSDPKLGSASADDIRYLAERIREAKLPVLLVGMRGSSEKE
                     TLAIRQLVEKTALPVVETFQAAGVISRELEAHFFGRVGLFRNQPGDMLLKRSDLVIAI
                     GYDPIEYEARNWNAEKDARIIVIDEAPAEIDPFMQPERELIGDISATLDLLTGSLEPQ
                     QVSEDAKEYLASLQAKLTERDIVQSKGEAGILHPLEVINTLQSKVTDDMTVTVDVGSH
                     YIWMARHFRSYEPRHLLFSNGMQTLGVALPWAISAALVRPNTQIVSVSGDGGFLFSAQ
                     DLETAVRKKLNIVHLIWNDGHYNMVEFQEKMKYQRASGVDFGPVDFVKYAEAFGAKGI
                     RATSVEELEKALEEGFATEGPVIIDIPIDYRDNEKLGETILPDQFY"
     gene            complement(7773..8435)
                     /locus_tag="IWO89_001409"
     CDS             complement(7773..8435)
                     /locus_tag="IWO89_001409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF624 domain-containing protein"
                     /protein_id="HAQ0456168.1"
                     /translation="MKEQRFDKNVYMEFFYWLYQSLASSLCFWCVNILYIAAAFLLSI
                     DGRNVFSFIFSLLFIGPGCIVMIGMTEELAKENSFSPVRLFIKMFRQFWLKGFFYWLF
                     AWIVSVIMIFDCFFFIRFSYGKWLIPLFVLLACLSVSFSTNCWYFQVRNPASKPNQVL
                     RIAFYYTLKKWYVSLLDFLLLTSLFLFFFVKPQWCILLGPSIVFGLIYFNNRKLMRTM
                     DL"
     gene            complement(8445..9260)
                     /locus_tag="IWO89_001410"
     CDS             complement(8445..9260)
                     /locus_tag="IWO89_001410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010731016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HAQ0456169.1"
                     /translation="MPFIQWLGMILLIVLALLTIIPFLWMVSASFKANNEVFTIPIQW
                     IPKTWHLENYLVIWSRIPLVTFFKNTAFLSVVITLIQLFTSSFAAYGFSKLNFKGRDI
                     LFLAYIGTIAVPWQSYMIPQFIMMRKVGLTDTLWSLVLLQAFNAFGVFLLKQYYSSIP
                     DSLCESARIDGLSEWGIYRKIILPLTKPAIASLTIITFVNTWNDYMGPFIYLSSTENK
                     TIQLGLKMFIGLFDAEYALIMAASVVSVLPVAFIFLAMQKYFVEGIASSGMKE"
     gene            complement(9521..9964)
                     /locus_tag="IWO89_001411"
     CDS             complement(9521..9964)
                     /locus_tag="IWO89_001411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator
                     substrate-binding protein"
                     /protein_id="HAQ0456170.1"
                     /translation="MLDPVDTEKYETILLPRKEKWGLLILENSVLAKKDVITKEDFAN
                     VPLLISKRPEIQRMFADWLGIKKEQLLITGTYNLIFNVFALIENQVGAALVIEGAVSH
                     HSKRVTFVPLDPMIETNCMLVWKKNRILSPVVNELIHRFKEAFEQ"
     gene            complement(10098..10436)
                     /locus_tag="IWO89_001412"
                     /pseudo
     CDS             complement(10098..10436)
                     /locus_tag="IWO89_001412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321036.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
     gene            complement(10424..10801)
                     /locus_tag="IWO89_001413"
     CDS             complement(10424..10801)
                     /locus_tag="IWO89_001413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HAQ0456171.1"
                     /translation="MNIKQVSEEKGISADTLRYYERIGLIPPVNRTKGGIRDYTEEDL
                     KWVDFTICMRNAGLSIESLIEYIRLSSEGEATVFERRKLLIEESELLTKKIAEMQECL
                     ERLQGKIKKYDRVLSRNKMECIK"
     gene            11025..12218
                     /locus_tag="IWO89_001414"
     CDS             11025..12218
                     /locus_tag="IWO89_001414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAQ0456172.1"
                     /translation="MTTYQRKVLVSTSAGIALENMDIMFLAFSLSSMITSFHISGTQA
                     GFISTITNLGMLIGGIFFGIIADKYGRVKVFSQTVLLFSIASLLMYFASNIYLVYLFR
                     FIAGVGAGGEYGACMSLISESFSKKQIGRASSVAGIGAQVGAALAAILAAVIIPTFGW
                     KMLYVVGVLPVLMVLFIRRGLTEPKEFKSVQSAEKKTKLSRLFESKHLAWQTCGLSIM
                     VTVQIAGYFGLMNWLPSIMQNRLGLSVSGSSLWMVSTILGMSLGMMTFGIIMDKLGPK
                     LAFSLFLICSSLSVYLLVLAHSQWSLVAAAVIVGYFINGMYGGYGAVISSLYPTEIRA
                     TANNFVMNLGRAVGGFSSIVIGFLMDHYSLTAVILFLSGIYLVSLLVLTTLTGVTALR
                     ESLQH"
     gene            complement(12384..12806)
                     /locus_tag="IWO89_001415"
     CDS             complement(12384..12806)
                     /locus_tag="IWO89_001415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
                     /protein_id="HAQ0456173.1"
                     /translation="MQTFLTKVSQKKIKMIHLLLETERWCSIEELQNELKVSSKSILN
                     YLTDLEELFQQYPDKVILKNENNRRFSMEKQENFPIYIIYLHFYRKSYNYHLIEFMYQ
                     HPEKNLEDYADAQYTSVSTVFRYAKLLVKYFEMTDVKE"
     gene            13389..13883
                     /locus_tag="IWO89_001416"
     CDS             13389..13883
                     /locus_tag="IWO89_001416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0456174.1"
                     /translation="MTEAESALSQAEANPTKENYATASAAIQALPENKQELTSRLAAI
                     DSTIKAKEAEEERKKQEEIARQQAEQAQQEAIAQQQAEQARQEAIAQQQAEQARQQAA
                     AEQASQQAQAAAPEQNNEQTVYVTPTGSKYHTHKCGNGTYTPATLSEAQARGLTPCSK
                     CYGN"
     gene            complement(14045..15217)
                     /locus_tag="IWO89_001417"
     CDS             complement(14045..15217)
                     /locus_tag="IWO89_001417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HAQ0456175.1"
                     /translation="MNKSRIRILDIFMAIILVVGIGIFSYPFVEDSLNDFLAQQMIIH
                     YQKQASNKNSEEIKKQQEKMTKKNQQLAEQNVSPGIGSFNQAVDAKALKDLPSNAFFM
                     EHMLGVIEIPKINVSLPIFDQTTEIFLQKGTSLLEGSSYPTGGKNTHAVLSGHRGLPE
                     AKLFTDLPKLKKGDQFFIQINGKTLAYQVEKIQVVLPDEVDSLGIQKGRDLVTLLTCT
                     PYMVNTHRLLVTGHRIPYQAKEAKKAIQGIDQWKKWKFFIWFIGILLGSIGLVWLLIA
                     YLDSLAIAKRNYPLSFYVKNTNGRPIEGMVFSVKTLNGKHYITREKVPFVKASDEYGL
                     VRFSDLKGRNYRLQHEELLLKIHVKHKHSKQFSMKLKKGRYKLRKEKEVYYLIEKE"
     gene            complement(15423..16238)
                     /locus_tag="IWO89_001418"
     CDS             complement(15423..16238)
                     /locus_tag="IWO89_001418"
                     /inference="COORDINATES: protein motif:HMM:NF033902.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaH/EbpB family LPXTG-anchored major pilin"
                     /protein_id="HAQ0456176.1"
                     /translation="MTGFDENTGVALYDPAESALTFTTDTEGSISVNGLIDGNYTLVE
                     TKAPDGYQLTNPQKETTFIVKNGKLQQTTGIIHGADEANLGNPNGVVNRENTTTDNTI
                     TVENVQNYGDFHFIKQDVNTQEKLADAEFSILRAKDPTVKLMKKIANLASGEYPYAWS
                     DEVTGSDWEEVKLISDSEGKFSIEGLKQGAYCLQETKAPEGYALPKNEAAYTEFTVDD
                     DPTTSDETTIDNQPKGILPSTGGMGIIVFILVGTALVGGAVIYFKKRHQETEA"
     gene            complement(16388..16834)
                     /locus_tag="IWO89_001419"
     CDS             complement(16388..16834)
                     /locus_tag="IWO89_001419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall anchor protein"
                     /protein_id="HAQ0456177.1"
                     /translation="MKNIKTKIWTFLGTAIMLLPFVLGLGTAEVSAAVSPTPENVTVN
                     LHKLKFTSAPENQINNGTELTFPNSEPLNGVEFNVYDITATYYPSKDTAVPADATPFA
                     SVTTSGEGLANLTLPGKSDGKDAVYVFVETPKPGVETSPNIVLSLP"
     gene            complement(16831..17835)
                     /locus_tag="IWO89_001420"
     CDS             complement(16831..17835)
                     /locus_tag="IWO89_001420"
                     /inference="COORDINATES: protein
                     motif:HMM:NF037639.1,HMM:TIGR04226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isopeptide-forming domain-containing fimbrial
                     protein"
                     /protein_id="HAQ0456178.1"
                     /translation="MTDSSNHKLTTIHLYPKSEQKIPAALTLTKTVENKQTDFADGDK
                     VPYLITVTIPENINEIGTYTIKDTADPQLWLEPETIDFLIGGDKIHTFHTQKIKHGFV
                     LSDSGKDLSSFAGKKLTIRYQMTLKENSEKTTFDNDVRLTTDNQTVETHLAIQTGGKQ
                     FVKVDRQTKQKLADAQFLVKKNDQYLAQENGINHWVAKDDSRATIFTSSKDGTFSVHG
                     LSFGSYELVEIKPPKGYILSQSTILFEVEKGSYDPLHPIPLEIINEPKANPTNNGKTP
                     VQMQTNGPSNEKNGGSFPKTSEEMLGGFSILGLLLVLSTGTAWFYKKQQKGNNRREIK
                     "
     gene            complement(17875..18204)
                     /locus_tag="IWO89_001421"
     CDS             complement(17875..18204)
                     /locus_tag="IWO89_001421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall anchor protein"
                     /protein_id="HAQ0456179.1"
                     /translation="MFESDKMPSISQNDGINIPDNFQGVKGLNGVTFAIYDVSDEFYK
                     LRSEGSSVEDAQRKLAQKSDSEKILAENVTKTVDEEEGIASFSASDKDEQGRDAVYRF
                     TEIKTSD"
     gene            complement(18330..20657)
                     /locus_tag="IWO89_001422"
     CDS             complement(18330..20657)
                     /locus_tag="IWO89_001422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HAQ0456180.1"
                     /translation="MKMIKKTQNKIQRICQKHFVSRLKAVSKRKYLHPIMISIILIFT
                     FLGSLSSIYVNVYTETSNFANQTTEETNSPPYSEIQSIESTNQSSNNETTSTTSSTQL
                     IDPTEKEITAAKAAGEKAYSETGRSQTFSTVAATANFNGHTIPDGYTVIYAKDGVIVA
                     VNGSMSADKTALQGVTKAIAFIDNRPDIHNLFIGSTVNGTTITFPKSIYMGVATAGNT
                     IDSVTPNADKSYSFTPSTWGGLPAGGGMSTFSIGIQLANDIPFTGYSIKNGQIVDGKG
                     NVSNFPTYQAATVQVNANRTGTFSYTVNGGAAKIGTYGTTLSGIHVGDKVVITPTAVT
                     GYSFAQTNGSFTALESNVDTVTYTGKQTPITIKYQDYFGQMIAPSKIMNLTYGSAAQD
                     LTTNTPTINGYTFTAVSAIETKNQSAMSVLASLDTNGNVIVKDANGKQISEVILYYKT
                     NATVSINANGSKYYDGLSVVPIVKYAFNDKTESTSLLNNDLSIDQITWNPTDFKAMNE
                     KGVEVTAPTEIGTYTSWQLTKSGLAKLAARTNYLFTIVQTSDVYTIKQISGAVELSGS
                     KTYDGKAGVPSIHVKLAEGVTSSLTPVQVALSSMDYTADAQSAKNMVNAGSYTIKLTN
                     SGIDKVKAANPKLSFTDIANTSGTYTIYKANAVITVDDAAFNYDAQSHSISVGNLHVT
                     GVVLGESLDYTLTVNSRTDIGSQIVTISLGEGSVNNNYTIIATDTAKLTINPTLLLPS
                     TGGIGLLPFVFLGLIFIFSGFFYFIHRKKKAGEQR"
     gene            complement(21500..21793)
                     /locus_tag="IWO89_001423"
     CDS             complement(21500..21793)
                     /locus_tag="IWO89_001423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2087 domain-containing protein"
                     /protein_id="HAQ0456181.1"
                     /translation="MVYTYTEQEKMKVLDTFMNDGKVKQVPSSTKKKYILLKEVLKRF
                     DHGVTYTETEVNSILLNVFSSGDYVEQRRYLITFGFFKRSSDGRAYQMMGIEN"
     gene            complement(22196..23374)
                     /locus_tag="IWO89_001424"
     CDS             complement(22196..23374)
                     /locus_tag="IWO89_001424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HAQ0456182.1"
                     /translation="MEKTDRFKKQISSTGRITWYFQAYRTTRRGFNSKREAQLAYLEM
                     EKQQRLKKKIVASNDKFSVVGEQWFQYYRSLNEQKEITYDKREEQLKIIVRWIGDKKL
                     SDLTPECLQELLFTLKECGVNGTNVGYAKNSLQSLIQVLNMIFKYCQRKNLISENPMK
                     SVRMPKYQLTVTDLKEAINNIEDKFLTVDELRTFLNYGIVHEELPMSVLFHVLFYTGC
                     RVSEALALQPGDIDFKRNEILFYKQTAVKGKSKDFRIETTKTVSSARRVLVTPLVMEK
                     LQQLIKALGEMRQHYRFIIEETYLFVYLDSSKRGVPYRREYVNDHVKRCVNRSGINKP
                     FHTHLARHTMASLVAEHCSWEVLKDRLGHTDQTTSKIYRHLTSNEKVKPLIAFSSLES
                     "
     gene            complement(23478..23792)
                     /locus_tag="IWO89_001425"
     CDS             complement(23478..23792)
                     /locus_tag="IWO89_001425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAQ0456183.1"
                     /translation="MELEVKTNVNSLEIEFYSILQKKLEDYTEKLLAVSHLPLVLTNT
                     DLKRLFQISDSTLNRLVKLGEFPPCWYGIRGHYFRDDVLEWMRKSDSNDYREKLRLLR
                     SL"
     gene            complement(24112..24507)
                     /locus_tag="IWO89_001426"
                     /pseudo
     CDS             complement(24112..24507)
                     /locus_tag="IWO89_001426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317574.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
BASE COUNT         7324 a         4812 c         4177 g         8268 t
ORIGIN      
        1 ccgactcccc aaacagtttg gatgacttgt ggtccgacct tttcgatttt ttgtcgtaat
       61 ttcttgatat gggcgtccac tgtccgttca tcaccaaagt actgataatc ccagactaat
      121 tcaagcaact gctcccgaga gaatacttga cgtggttttt ttgctaaagt aaacaataga
      181 tcgaactctt taggcgtcag tccttcaatt ggccggttgt ccagatacgc ttctcgtgtt
      241 ttcgtattca tcttgaaatg actagtctcg atatcaaaat gatcatctga aactgctgct
      301 tcttctcttg cttctgccaa ctcactacgg cgatgcaaag ctttcattcg agcaataagc
      361 gtcagaggac tgaaaggttt tgtgacataa tcatctgccc ccatctctaa gccgatcacc
      421 tgatcgcttt cagaatctct agctgtcagc atgataatcg gcacagtact tgaaactttg
      481 cggatctctc ttcctactag cataccgtcc agtgtgggca aattcaggtc tagtgtaatc
      541 atatcccaat gttgtgggtt ttctaaaaag gcatcgagcc cttcttttcc atcatacttg
      601 aaagttgctt cccaaccttc atttaagaaa aacatctgca tcatttcaga aacagattga
      661 ttatcttcaa tcattaaaat attcattatc gttccctcca gtttccaggt catatttctg
      721 taatttcttt tctgtcataa taaaaatagg cataattgga aatactagca gaagcatttc
      781 aatcagaaaa taattcgtat gcccagatgt ctttaaataa tagccgatca atgccatcag
      841 taagcccata agaaaaaacc agtttccgct tgtcttttga gccagccgcc aatgagcgtc
      901 tgtttttgcg gcaagaggcg aatgataccc ataaaaaggc agatttcctt ttgagggcag
      961 aatccgaaaa agactgccaa ttattaagaa taagatacca atactgaaaa aaatcattct
     1021 ttccctccta aattctcttc ttatagtata ctctaaattg ttgactgaac caatatgttc
     1081 aggaattctt cacgaaaggt ttgtcttttg ttcattcttt gttaagataa acttattcca
     1141 ataaaaaagg agttttattt gtgtcaaatt atcgtgcaat cactttcttt gatcttgatg
     1201 gaacattact agatgcgaaa tcaaaaatca cacccgaagt ggcaaaagct atgaacgcat
     1261 taaaagaaaa caatgttctc cccgtcatcg ctacaggacg tacagaagca gaaatccact
     1321 ctatcatgga agatgcgggc attacttctg cggtcaccat gaatggttct ttcattcgag
     1381 tagacggcga agaaatctat tctcaaacat tttcttcaga agaatgtcaa aaaatgtatg
     1441 atcatgtaca gttgaacggt catgaacttt cttactataa cgatcagaaa atctggtgta
     1501 ccggtcatag ccaaacaatg atcaatgctt acgacttcat tcattcagat gttccggaaa
     1561 ttgatccttt aggctttaaa gaaaacacag taaatatgtt gctgatctta tctcaatcag
     1621 gtgacgaata ctatcacgaa cattttcctg agctaacttt ttatcggaat ggtccttact
     1681 cgatcgatac agtgaagaaa ggaatctcaa aaggggcagg tgtcaaacgt ctgcaaaaag
     1741 aacttcagct ggaaaaagtt cctacttttg gatttggcga tggtccgaat gattttgcct
     1801 tgttggaagc ttgcgacaac aaaatcgcta tgggcaacgc ctatgacgga ttaaaagaac
     1861 tttctacctt tatcacaaag aaaaatacag aaggcggaat tgtccacgca ttgaagtatt
     1921 ttgatctgat ttaagatctt tttttgaagc tacctctttt cttaagaaaa tcgcttattt
     1981 tttcttaaga aaggggcagt tattatataa ataatgggat atgattcgct tttttattga
     2041 atttatccca atatatgcta tactaatttt gtatgaaaaa tacacgtaac ccttgttagc
     2101 taacggggac gttacggatt cgacaggcac agtcgagctt gaattgcgtt tcgtaggtta
     2161 cgtctacgta aaaacgttac agttaaatat aactgctaaa aacgaaaaca actcttacgc
     2221 tttagctgcc taaaaacagt tagcgtagat cctctcggca tcgcccatgt gctcgagtaa
     2281 gggtcctaac tttagtggga tacgtttcaa ctttccgtct gtaagttgaa aaagagaaca
     2341 tcagactagc gatacagaat gcctgtcact cggcaagctg taaagtgaat ccttaaatga
     2401 gttgactatg aacgtagatt tttaagtggc gatgtgtttg gacgcgggtt cgactcccgc
     2461 cgtctccata tatgtatggt tgagacagat aaacatgtat gaaaaacgtt gttaaatcaa
     2521 cgttttttta ttttggtcaa ggagagaaaa gtagagaaat acaaaatttt ttgcattgat
     2581 tttgcattca aaaaaacatt caaacattta gaaatcccca atacgtattc aggaaaatct
     2641 attttaaggt ttacaaaaag aacttttgtt cgtacactct ttttgaggtg aacttttatg
     2701 ctgatggaag gaaaaacaca attatggttt aagcatgttc cttcaaataa gttttttaat
     2761 tataaactct agagaaatta attcatccta acataaaata attgttcact caaaagtttt
     2821 tctagaacaa ttaaaacagc aggcttcaca ttcgctatac atttgtaaga agtaataatt
     2881 cctgtactaa aaggcataat tgctttaatt attttttgac ttctcttcaa acacaagaag
     2941 tagttgtgta ataattgcag aaataaggat tccaataata gatataaaat ttgacatgga
     3001 aacgtctata acattaaaag aataaagtgt tgaagaaagt aaactgattg aaataacccc
     3061 tccgtaaaaa atagtctgtg aatataccca ctttttttca gaagacgtac ttcgtttggt
     3121 tctaaaacct atccctgcat ttctactctt atcaaaaaac caaatattta gaagtaataa
     3181 tattgctatt attaagataa aattactgta aaaatccaaa agctatcctc ctagcaacta
     3241 tttaaatacc caaaaaaatt acctctagat aatatcatac ctacgttttc acttataaat
     3301 tttttcataa aaaaagcgtt tagaggcata atcgttaata cagatggtaa ccaatgtatc
     3361 tggtttgaac tgggatatca ccctctccat atatgtatta ctaagactaa tagatactat
     3421 aaaagacttg atagttttgc attgattttg cctaatactg agcataggtg tatgctaaac
     3481 gatactataa ggagtgtgta cataatgaaa agaattctgt ttatcagttt atcaaattta
     3541 ttgttactta ctgcttgtgg gacttctgca tcaaagaaca taccttcaga tacgcaaatt
     3601 tcgaatagta tcgattcaac aatttctaca aatgagaaaa attctccggt agaaacttcc
     3661 gatataaaag tttctacctc tgcaaaaaac agggtaaaga aactgtcagc acgtctcctt
     3721 tatttcaaac aaactctgaa ttagcttctt catcttcttc agcaaataat gatgatagtt
     3781 tcgcgtttac tcctaactaa atgcaacctt tacaaatgct ttacgataac aagcctgagc
     3841 taaatgatcc tgatatcagt tttgcatttt ttgaaatgat tggatcggat gatcttttta
     3901 aaactactag tttgtctatc cgaaaatagg acagttctgg cagtataggt ttttatcgag
     3961 tttctccacc aggcctcgtt acagagacag attctctagg cgaacctttt gaataaatca
     4021 aaaatcatga acaatcactg ccaactaaat aaagtgcctt caaaagcttg gtcttctcgc
     4081 ttttgaaggc actttattta aatctttatg aaactaaagt atgttcatat ttgttgctat
     4141 agttatatag atattctgtt gtttccaact cataaacgtg agtatatttt gccactactt
     4201 ttccgcattt ttctaatatt tcattgcgat cgacgtcatg atattcgtct acgcagatca
     4261 caacacaatt ttctaattta ctgattactt ctccaaccac tttttgtttc aagccaatag
     4321 gttggcaaat atattttttt cctaaaatga tagtttcgtt attcatattt catccatcct
     4381 taatgttttt tcattattca aaatgataat accattaaaa tatgattgtg aaaatcaata
     4441 acacttgtga ttttataaaa aaataaaaaa accgaaaaac agaaatgacc actgtctttc
     4501 cggctctttt tattcttgat ttctcttctt ttttgtttca atagacgatt ttagttatta
     4561 ataaatgaaa aagcatttat attaaaattt gctaataaaa gatctacata tttttctaga
     4621 aatgcttgat cgatttcatc ataccctttc gttactccgc aatcgatatc taaaacaccg
     4681 attaaattac cttcttttac cattggcact acgatttctg attgtgcggc tgaatcgcag
     4741 gaaatgtaat ttgcatgggt tttgacatca tcaacgatca atgtttcttg tttttcagct
     4801 gattctccac aaactccttt tcccatcttg atacgtgtgc aagaaactcg cccttggaac
     4861 ggtcctaaaa tcaattcatt accatcgaat aaataatatc ctgcaaagac agtctcaggc
     4921 aaagtctcat tcaacaatgc agatgaattt gctaaatttg ctaaccagtt atcttctgct
     4981 tctaataatc cttgttgctg taataataaa atttcgtatg catctttttt ctgtttttct
     5041 gtccacatga taaaaaccgc tcctttttga tctatggaga agattatagc tcagtttttt
     5101 tgtaaacgct aggtttcctc ctcaaaaaaa cgagatacgt caggttaatc accttgaacg
     5161 catctcgctg tctttttcta ttctgcttgt tggatttctg ctgctaaatc agcataatca
     5221 atcgtttcgt gaacaaaagt cggatcatca actggaaaat gctgtacaag ggacgagatg
     5281 cggctaatct caacttttcc ttcagccact tcaaaatcca ttacatgtcc gccaaatgta
     5341 tgtgtatcat cgataaaatg caggtgaaat cccgctgcag caactccttg gaataattca
     5401 ggcgtaaaga acccgactaa tgttccttgg atattgcttt gttggaattc tggttgagac
     5461 tctgaaattt ttgctagtct tctgtaaggt ttttcttgct tcggcatgat acggatgtgc
     5521 atatttttga ataatccagt tatcttcact gccaaaaata ggttttcccc ggcttctctt
     5581 actaatatgt cttttttcaa ttgttctcca tctgcagttt ttgagacaga aaaactagta
     5641 tctggcgtaa aatctgtaat tgctgcatag ggggtaagtt cacttccatc taattgggca
     5701 actgagccat ctgatcttcc ttgataagct acgccatcta aaaagatgac ttcaccatca
     5761 agcccatgca atgttcccaa tcccagagtt cctttttcta atatatgagc aatcgtttct
     5821 gttccatcca tcaagccggc catcaaagct cctaatgtcc cgtgctggta taagattttt
     5881 tcagtcatgt cgacacttcc ttaatagaat tgatctggta agattgtttc tcccaatttc
     5941 tcattatcac ggtaatcaat cggaatatca ataattacgg gtccttctgt cgcaaatcct
     6001 tcttctaaag ctttttctaa ttcctctaca cttgtagctc ggattccttt agcgccaaaa
     6061 gcttctgcat atttcacaaa atcaactgga ccaaagtcta cacctgacgc ccgctgatat
     6121 ttcatttttt cttggaactc taccatattg tagtgtccat cattccaaat caaatgaacg
     6181 atattcaatt ttttacgtac cgccgtttct aaatcctggg cagagaataa aaatccccca
     6241 tcgccggaaa ctgatacgat ttgtgtattt ggacgcacca atgcagcaga aatcgcccat
     6301 ggcaatgcaa caccaagcgt ttgcatacca ttactgaata acaaatgacg aggttcataa
     6361 cttctaaagt ggcgtgccat ccaaatataa tgactaccta catctacggt aacagtcata
     6421 tcatctgtta cttttgactg aagcgtgtta ataacttcta aaggatgcaa gatacctgct
     6481 tctccttttg attgaacaat gtcccgctcc gttaattttg cttgaagaga tgctaaatat
     6541 tcttttgcat cttctgacac ttgttgaggc tctaatgaac ctgtcaataa atcaagagtg
     6601 gctgagatat caccgatcaa ttctctttca ggctgcataa aaggatcgat ttcagctggc
     6661 gcttcatcga tcacaatgat ccgcgcatct ttttccgcat tccagtttct tgcttcatat
     6721 tcgattggat catagccaat tgcaataact agatcacttc gtttcaaaag catatctcct
     6781 ggttggtttc taaataaacc cacacgacca aagaaatgcg cttctaattc acgtgagatc
     6841 actccagctg cttggaatgt ctctacaaca ggcaatgctg tcttttcaac taattgcctg
     6901 atggctaacg tttctttttc acttgatcct cgcataccta ctaatagtac cggtagtttt
     6961 gcttctctaa tgcgttcagc taggtaacgg atatcatctg cagaagcaga acctaattta
     7021 ggatcgctca atggcttgat gacattacct tggacaggtg catccactac atcttgcggg
     7081 atactgataa agctcgcgcc tttttttgaa ctcttagcca tacgataagc attggcaatc
     7141 acttctgaaa ttgtttctgg atcttggatt tctgcactgt attttgtgat tggctggaac
     7201 agtgctgcat tatccatgct ttgatgggtc aacttcaata gatcgcttcg ttttacttgt
     7261 ccggcaattg ctaaaactgg gtcaccttct gctgtcgctg ttaccaaacc tgtagctaag
     7321 ttactagcac caggaccact tgttgcgata actactcctg gctctcctgt aatacgtccg
     7381 attgcttgtg ccataaatgc agcattttgt tcgtgacgag tcacgattaa ttcaggacct
     7441 tgatcctcta attcattaaa tacaccatcg atttttgcac ctggtatacc gaatacataa
     7501 tcaacatgat gattaatcaa actttctact acagatttcg aaccttgttt cattttttca
     7561 gccattgaac atcgtccttt ttatgcttta tttttaatta ttagttagaa ataatcacaa
     7621 ataagtgata cagtttctaa acctgtttca tacaaaagta tagcacgttt tatttcattt
     7681 gtttagccat atgtttcaaa aaatgaaaaa gaatgacagt ttttacataa aaaaaactgt
     7741 cattcttaat tgaaagtttc aatttataaa gtctacagat ccatagttct catcaattta
     7801 cgattgttga agtaaatcaa accaaatacg atagaaggac ctagtagaat acaccattgg
     7861 ggctttacga aaaagaagag aaacaaactt gtcaatagta aaaaatctag taaactcaca
     7921 taccattttt tcaacgtata gtagaacgct atccgtagaa cttgatttgg tttcgacgct
     7981 ggatttctca cctgaaaata ccagcagttg gtgctaaagg aaactgataa acaagcaagt
     8041 aaaacaaaca acggaatcaa ccattttcca taagaaaaac gtataaaaaa gaaacaatca
     8101 aaaatcatta tcacactaac tatccaagca aatagccaat aaaaaaatcc ctttagccaa
     8161 aattggcgaa acatctttat gaacaaacgt actggtgaaa aactattttc ttttgctaat
     8221 tcttctgtca tacctatcat cacgatgcaa cctggaccaa taaaaagtaa agaaaaaata
     8281 aaggaaaaga cattccttcc atctatcgac aaaaggaacg cagctgctat atacaagata
     8341 ttgacacacc aaaaacaaag gctacttgcc aatgattgat acaaccaata aaagaattcc
     8401 atataaacat ttttgtcaaa acgctgctct ttcataatca cctcctattc cttcattcct
     8461 gatgaagcga tcccttctac aaaatatttt tgcatcgcta aaaatataaa agcaacagga
     8521 agaacagaaa caactgaggc agccatgatt aatgcgtact ctgcatcaaa tagcccaata
     8581 aacattttca accctaactg gattgtttta ttttccgtag aagacaaata aatgaatggc
     8641 cccatataat cgttccatgt attaacgaat gtgatgatcg tcaaactggc aatcgccggt
     8701 tttgtaagag gtaaaatgat cttgcgatag attccccact ctgataagcc atcgatccgc
     8761 gcactctcac ataaagaatc tgggatacta ctgtaatatt gcttcaacaa gaacacacca
     8821 aaagcattga acgcttgtaa tagcaccaac gaccacaacg tatctgttaa acccactttt
     8881 ctcatcataa tgaactgcgg gatcatataa gactgccaag ggacagcaat cgtgccgata
     8941 tatgctaaaa ataaaatatc gcgcccttta aaatttagtt tagaaaaccc gtaagcagca
     9001 aaactagaag taaacaactg aatcaacgtg atcacaacgc ttaaaaaagc tgtattctta
     9061 aaaaatgtca ctaaaggaat acgagaccaa ataaccagat aattctctaa atgccaagtt
     9121 ttagggatcc attgaatcgg aatcgtgaac acttcattat ttgctttgaa tgaagcagaa
     9181 accatccata aaaaaggaat gatcgtcaat aaagctaata caatcagcag aatcataccc
     9241 aaccattgaa taaagggcat gttttttgaa cgaatttttg ctttttttct ttcatttcga
     9301 acacctccaa agttataatc ttcatttctt tgtaatgatt acaatccaga acaacggtta
     9361 ttatagctga ttcatttccg ttcttcttat ttttgtaagc gcttatttaa caagttatgt
     9421 aaatctcttt atttttttga tttaaaaatg aaaaaataca aaagcaagac tgtcatacgt
     9481 ctcgcttttg tattgaaatt tcttcgatta gagatactta ttactgttca aaagcttctt
     9541 tgaaacggtg aatcaattca ttgactacag gtgataaaat ccgattcttt ttccagacta
     9601 gcatgcagtt cgtttcaatc attgggtcaa gcggaacgaa tgtcactcgc tttgaatgat
     9661 gacttacagc accttctatg actaaagcag cacctacctg attctcaatt agcgcaaata
     9721 cattgaaaat caaattgtac gttcctgtga tcagcaactg ctcctttttt atgcctaacc
     9781 agtcagcaaa cattcgctgt atttctggac gctttgagat caaaagaggt acattcgcaa
     9841 agtcttcttt tgttatcacg tctttttttg ctaaaacaga attttctaaa atgagcaatc
     9901 cccatttttc ctttcttggt aacaaaatcg tttcgtattt ctctgtatct actggatcta
     9961 gcaaaatcgc taaatctaac aggctttttt ctagtcactc cgtgatatca tcacttgttc
    10021 cgctgtggat atgaaattga acgctgggat aatcacggat caacttttct agtaatagtg
    10081 ccaatgtatt cgaactatca gcttccacac agccgatcgt aaatcggccg ctgaataatt
    10141 gtttttttct gttctcgaat tcttgagtgg tttgattcgt taaatctaaa atttcttctg
    10201 ctcggctttt ttagaaaata acctgcttct gtcaagatca ttttgcggtt ttcgcgtaca
    10261 aacagttctg ttcctagtta ctcttctaac tccttcagtt gtcggcttaa ggttggttgt
    10321 gtaagatgta gaacttctgc ggcttgagag attgttcctt cttctgcaat tgtccaaaaa
    10381 tattttagta agcgtaactc catagcgatg ctccttgatt tttttacttg atacattcca
    10441 ttttatttct agataacaca cgatcatatt tcttgatttt tccttgtaaa cgttccaaac
    10501 attcttgcat ttcagcaatt tttttcgtca aaagttcact ttcttcaatc aaaagcttac
    10561 gccgctcaaa tacagttgct tctccttcgc tagataggcg aatatattca atcaaagact
    10621 cgatcgataa tccggcattt cgcatacaga tcgtaaagtc gacccatttt aggtcttctt
    10681 ctgtataatc acggattccg ccttttgtcc gattaactgg tggaatcaac ccgattcgtt
    10741 cataatagcg caatgtatcg gccgaaatgc ccttttcttc actgacttgt ttgatattca
    10801 tctgatcact ctttctctta atttcttgcc actttactta tgtattttat ttattctaca
    10861 actttaagtg tactccaagt caatactcat ttctattaaa actaagctgg attatctata
    10921 tcggttgttt gtgtattatt tcaaataaaa aagtcttgtt tttttattct tttttggata
    10981 tacttttaac aatttaggga aattttagga aaaggggtat aaaaatgaca acgtatcagc
    11041 gaaaggtatt agtatcgaca tcggcgggaa ttgctttaga gaatatggat attatgtttt
    11101 tagctttttc attatcttca atgattactt cttttcatat tagtggtacg caagcgggtt
    11161 tcatttcaac gatcactaat ttggggatgc tcattggagg tatttttttt ggcatcatag
    11221 ctgataaata tggacgtgtg aaagtatttt ctcaaacggt ccttctgttt tcgattgctt
    11281 ctttattgat gtattttgct tcaaacatct acttggttta tctctttcgc tttatcgcag
    11341 gtgtaggagc gggaggagaa tatggggcgt gtatgtcctt gatttccgaa agtttttcaa
    11401 aaaaacaaat tggacgagct tcttcggttg ctggtattgg tgcgcaggtc ggtgcggctt
    11461 tggctgcgat tttagctgct gtgatcattc caacgtttgg ttggaagatg ttgtatgtcg
    11521 taggtgtact gccggtgttg atggtcttgt tcattcgtcg cggcttgact gaaccaaagg
    11581 aattcaaatc tgtacaatct gccgaaaaga aaaccaaact tagcagactg tttgaatcaa
    11641 aacatcttgc ttggcaaaca tgtggcctta gtatcatggt aactgtccaa attgccggct
    11701 attttgggtt aatgaattgg ctaccttcaa ttatgcaaaa tcgattaggg ctttcggttt
    11761 ctgggtcttc attgtggatg gtcagtacta ttttaggcat gtctttgggg atgatgactt
    11821 ttggcataat catggataaa ttggggccta aactagcctt ttctttgttt ttaatttgtt
    11881 catccttatc tgtctatcta cttgtgttag cccatagcca gtggagtttg gtagcagccg
    11941 ctgtgatagt cggctacttt atcaatggca tgtatggtgg atatggcgca gtgattagta
    12001 gtttgtatcc aacagaaatc cgcgcgacgg cgaataattt cgttatgaac cttggacgag
    12061 ctgttggtgg tttttcttct atcgtcatcg gtttcttgat ggatcattat agtttaactg
    12121 ccgtaatctt gtttttgagc ggtatttacc tagtcagtct gctggtcttg acaactctta
    12181 ctggtgttac agcattaagg gaaagcttgc agcattgata gttgagtctg taaatgacaa
    12241 gaaagaggtg tggaaaaagc ttgcatctgc ggtaataaga ccagccaaat caaaaacatg
    12301 agacactgtt tccgtttggc tggtcgtatt acttgatgca gggcagcttt ttcaactgct
    12361 aatttccttc tatctggcgg cgactattct ttgacgtccg tcatttcaaa gtactttact
    12421 aataacttag cataacgaaa aactgtggat acactcgtat attgagcgtc tgcataatct
    12481 tccaaatttt tctcaggatg ttgatacatg aattcaatca aatgataatt atatgatttt
    12541 ctgtagaaat gaagataaat aatgtagatt gggaagttct cctgcttttc catagaaaag
    12601 cgccgattat tctcattctt caaaatgacc ttatcaggat attgttggaa caattcttct
    12661 aaatctgtta gataattcaa gatagactta cttgatacct tcaattcgtt ttgtagttct
    12721 tctattgaac accaacgttc tgtttcaagc agtaaatgaa tcattttaat tttcttttga
    12781 gaaacttttg ttaaaaaagt ctgcatattt tctcctcctg tatctacttt actatactac
    12841 atacaagaaa aaaacggata caaatatcat attgtcacac cgtcaatagt gcataaacct
    12901 gttattattt ctcttttttt cctaagtatt ctattgatat gaatcattta atcgtttatt
    12961 atataaatgt caataatcta ataataaaat aacgtacata ttttattatt gcatacttaa
    13021 taacagaact atagcaaaaa atcccgtgta tcaacacggg attagattaa gtttcctcaa
    13081 tcactattca taacacaatt attataacaa gtaagtaaac aattgaaaag gacatttatt
    13141 gaaaacggtt gttttttact taaggaggaa atttagtgaa aaaattagtt gcgttgtttt
    13201 tggtcgcagg aagtttgttg agtgggtgtg gtgctactac gtctactact caaagcacaa
    13261 gttcagctca ggcttccagc agcagtacca cgatctccga aagtacggca gattcatcga
    13321 aagaacaaat tgaaaagaaa aaagaagaag agaaacaaaa agcggaaaaa gctgcactag
    13381 aaaaaaaagt gacagaagcg gaatctgctt tgtcacaagc tgaagcaaat cctacaaaag
    13441 aaaactatgc aactgcttca gcagctatcc aagcgctacc tgaaaacaaa caagagttga
    13501 caagccgtct tgctgcaatt gattccacaa tcaaagcaaa agaagcagaa gaagaacgga
    13561 aaaagcaaga agaaattgca cgacaacaag cagaacaagc gcaacaagaa gcaatcgcac
    13621 agcaacaagc agaacaggcg aggcaagaag caatcgcaca gcaacaagca gaacaggcgc
    13681 gacagcaagc agcagctgaa caggcttctc agcaggcaca agccgcagct cctgagcaaa
    13741 ataatgaaca aactgtctat gtcacaccga ccggttcaaa ataccataca cataaatgtg
    13801 gaaatggtac gtatacccct gccactttaa gcgaagctca agctcgtggt ttaacccctt
    13861 gctccaaatg ttatggaaac taattcaaaa gttatactat aataataaaa ttcttctgaa
    13921 acataaaaaa atagaagctg gcgttccaga tagtaaacca aaaatagcag tttacacgtc
    13981 catttcgtca gcttctctct tttttcattt agtctgttcc ctctgctttt ttggacatct
    14041 tcattcattc tttctcaatc aaatagtaca cctctttttc ttttcgcagt ttatatcttc
    14101 cttttttcaa tttcattgaa aactgtttcg aatgtttatg ttttacatgg attttaagca
    14161 ggagctcctc atgttgcaag cgatagttcc tgccttttaa atcagaaaat ctcactagtc
    14221 cgtattcatc agaagctttc acaaatggaa ctttttctct agtaatatag tgtttgccgt
    14281 tgagcgtttt tacagaaaag accattcctt cgattggtcg tccgtttgtg ttctttacat
    14341 aaaaagataa aggatagttt cgcttagcga tggccaaaga atctaaataa gcgatgagta
    14401 gccaaactaa gccgattgat ccaagtagta taccgataaa ccaaataaaa aatttccatt
    14461 ttttccattg atcaattcct tggatcgctt tttttgcctc ttttgcttga taaggaatac
    14521 gatgccccgt cacaagcagt cgatgtgtat tgaccatata aggagtgcag gtaagcaaag
    14581 tcacgagatc tctgcctttt tggataccca aagaatctac ttcgtcaggt aagaccactt
    14641 ggattttttc tacttgatac gccaatgtct ttccattgat ctggatgaaa aactggtctc
    14701 cttttttcaa cttaggcaaa tctgtaaata attttgcttc tgggagtcca cgatgcccag
    14761 aaagaacggc atgggtgttt ttcccgccag tcggataaga acttccttcc aataatgacg
    14821 ttcctttttg caaaaatatt tccgttgtct gatcgaatat aggtaagctg acattgattt
    14881 ttgggatttc gatgactcca agcatatgtt ccataaaaaa ggcattacta ggtaaatctt
    14941 ttaatgcttt tgcatcaact gcttggttga aactgcctat ccctggtgat acgttttgct
    15001 ctgccaactg ttggtttttc tttgtcattt tttcctgctg tttttttatt tcctctgaat
    15061 tcttattcga tgcttgcttt tgataatgga tgatcatttg ctgggcaaga aaatcattca
    15121 gtgaatcttc tacaaaagga taagaaaaga tcccgatccc aacgactaaa atgattgcca
    15181 tgaagatgtc cagtattctt attctgcttt tattcatcat gtccacctat tttctaatat
    15241 gtatatgaag aggttgtgaa atcagcgcct tgacctgagt attagcgaag caaacggcaa
    15301 aatagttcct cctattttgc cgtttgttga gcgaatacca agagaccgac cacttgtcac
    15361 aacctttctc cccatttgaa tcactatcgt ttatttatcg tatgattttt gtttccaata
    15421 gtttaagctt ctgtttcttg atggcgtttt ttgaaataga tcactgctcc tccaactaaa
    15481 gcagtaccta ccaagatgaa tacgatgatc cccataccac cagttgaagg caagattcct
    15541 ttaggttggt tatcgatcgt tgtttcatca gaagttgttg gatcatcatc tactgtaaat
    15601 tcagtgtacg cagcttcatt cttaggtaat gcgtagcctt ctggtgcttt tgtttcttga
    15661 aggcagtatg ccccttgttt cagcccttcg attgaaaatt tcccttctga atcagaaatc
    15721 aatttcactt cttcccagtc agaacctgtg acttcatctg accaagcata tgggtattct
    15781 cctgaggcaa ggtttgctat ttttttcatt aatttaacag ttggatcttt tgctcttaga
    15841 atagaaaatt ctgcatcggc taatttttct tgcgtgttca catcttgttt gatgaagtgg
    15901 aaatcaccgt agttttggac attttctaca gtaatcgtgt tatccgtagt tgtattttcc
    15961 cgattgacta caccgtttgg attcccaaga ttggcttcat ctgcgccatg gatgataccg
    16021 gttgtttgtt gcaattttcc gttttttact ataaaagtag tttctttttg tggattggtt
    16081 aattgataac catctggtgc ttttgtttca actaaagtat aattgccgtc tattaatcca
    16141 ttgacactga tggatccttc agtatcagtt gtgaatgtca acgctgactc agcaggatca
    16201 tagagagcga caccagtgtt ttcatcaaaa ccggtcacga attgattctc tgaatttttg
    16261 atctggaatt gcgcccctgc taacttatct acccctgaaa agttcccagc tttctggatt
    16321 ttgatattgc tttctttgat gacattttta gcatataggt gaatcgtgtc taaaggtttg
    16381 tcagggtcta gggtaagctt aacacgatat ttggcgacgt ttcgacaccg ggctttggtg
    16441 tttctacaaa gacgtataca gcatctttcc catcagattt acctggaaga gtcaggttag
    16501 ctaatccttc accagaagtc gttacacttg cgaagggtgt agcgtctgct ggaactgctg
    16561 tgtctttact tggatagtac gtagcagtga tgtcatatac gttgaattca acgccgttca
    16621 acggttcgct attcggaaat gtcaattccg ttccgttgtt tatttgattt tctggtgctg
    16681 aagtgaattt caatttatgc aaattaaccg taacattttc tggtgtaggt gatacggctg
    16741 cagaaacctc tgcagttcct aggccgagaa cgaaaggaag cagcatgata gcagttccta
    16801 aaaacgtcca tatttttgtt ttgatgtttt tcattttatt tccctcctat tgtttccttt
    16861 ttgttgtttt ttataaaacc aagctgtccc tgttgaaagg actagcaata accctaaaat
    16921 tgagaaacct ccaagcatct cttcgcttgt ttttggaaag cttcctccat ttttttcatt
    16981 tgatggacca tttgtctgca tctgaactgg cgtttttcca ttattcgtcg gatttgcttt
    17041 tggttcgttg atgatttcca atggtatagg atgaagcgga tcataactgc ctttttcaac
    17101 ttcaaagaga atggttgatt gactcaatat atatccttta ggcggtttaa tctccactag
    17161 ttcgtaactt ccaaaagaca atccatggac agaaaatgtt ccatctttac tggaagtaaa
    17221 aatcgttgct cttgaatcat ctttggctac ccaatgattt atcccgtttt cttgcgctaa
    17281 gtattggtca ttttttttga ctagaaattg agcatctgct agtttttgtt tcgtttgccg
    17341 atccactttg acaaattgtt tgcctcctgt ttggatggca agatgtgttt ctactgtttg
    17401 attgtcagtc gtcaaacgta catcgttatc aaatgtcgtt ttttcactgt tttctttcaa
    17461 tgtcatttgg taacggatcg ttaatttttt tccagcaaac gaactaagat cttttccaga
    17521 atctgataag acaaaaccat gttttatttt ttgtgtgtga aatgtatgaa tcttgtctcc
    17581 accaatcaaa aaatcgatcg tttctggttc tagccataac tgaggatcag ctgtatcttt
    17641 gatcgtatag gttcctatct cgttgatgtt ctcaggaatt gttacggtga tgagataagg
    17701 gactttatcc ccatctgcaa aatctgtctg cttattctct actgtttttg ttaatgtgag
    17761 tgcagcaggt attttttgtt cgctctttgg atacaagtgg atggtcgtca atttatgatt
    17821 agatgaatct gtcacaggaa gcacaacgac aaatggtgct gatttttctt ttacttaatc
    17881 tgaagtcttt atttccgtaa atcggtaaac agcgtcacga ccttgctcat ctttgtccga
    17941 tgcactaaaa gaagcaatgc cctcttcctc gtctactgtt tttgtcacat tttctgccag
    18001 tattttttca ctatcacttt tttgtgctag ttttctttgt gcgtcttcca cactacttcc
    18061 ttctgaccgc agcttgtaaa attcatccga gacatcatat atagcgaaag taacgccgtt
    18121 caaccctttt actccttgaa agttatccgg gatatttatt ccgtcgtttt gactgatact
    18181 tggcatctta tcgctttcga acattcgatt atgcaaagta aatgtaatag gtgattctct
    18241 agctgacaca gtcggagaac ctataaaaaa gaccagtagg ataaaaaaga gaacagcaaa
    18301 tatatccaca cgtacatttt ttgttttttt catcgctgtt cacctgcttt tttcttacga
    18361 tggataaaat aaaagaagcc agaaaaaata aagatcagtc ctaaaaagac aaagggcagt
    18421 agacctatcc cacctgtcga aggaagcaag agagtcggat tgatcgtcaa cttagcggta
    18481 tctgttgcga taatcgtata attattgttt acacttcctt cgcctaaact aatcgttact
    18541 atttgtgatc caatatctgt ccggctgtta actgttaggg tataatccaa tgattcgcct
    18601 aagacaacac cggtaacgtg gagatttcca accgaaatcg agtgactttg ggcatcataa
    18661 ttaaaggcag cgtcatcgac agtaatcact gcgttagctt tatagatggt ataagttcct
    18721 gatgtgttgg ctatatcagt aaatgacaac ttgggatttg ctgctttgac tttgtctatt
    18781 ccgctattcg ttagtttaat tgtatagctg ccagcattaa ccatattttt tgctgattgt
    18841 gcatctgctg tgtaatccat actgctaagt gccacttgta ctggagtaag cgaactagtg
    18901 acaccctctg ctagcttgac atggattgac gggacacctg cttttccatc atatgtctta
    18961 ctgccactga gctctaccgc accgctgatt tgcttaatcg tatagacatc agatgtctgc
    19021 acgatagtaa ataaatagtt tgttctggca gctagtttag ctagtccgct ttttgtcaat
    19081 tgccaagacg tataggttcc aatttcagta ggcgcagtta cttcaactcc cttttcattc
    19141 atcgctttga aatcagttgg attccatgtg atttgatcaa tactgagatc attgtttaaa
    19201 agggatgttg attctgtttt atcattaaag gcatatttaa cgatcggtac aacagaaaga
    19261 ccatcatagt acttactgcc atttgcattg attgatactg ttgcatttgt cttataatat
    19321 aggatgactt cagaaatctg ttttccattt gcgtccttta cgattacatt tccatttgta
    19381 tctaaactag ctaaaacact cattgctgat tggttctttg tttctatcgc tgaaacagct
    19441 gtaaaggtat atccgttgat agtaggcgta ttagtcgtca ggtcttgtgc agcacttccg
    19501 taagtaagat tcattatttt agaaggagca atcatctgtc caaaatagtc ttgatactta
    19561 atagtgattg gtgtttgttt gcctgtatag gtcaccgtat cgacgttact ttctagggct
    19621 gtgaaagaac cgtttgtttg tgcaaaagaa tagcccgtta ctgctgttgg agtgataacc
    19681 actttgtcac ccacatggat tccagataat gtagtcccgt acgttccgat ttttgctgct
    19741 ccaccattga ctgtataaga aaaagttcct gtccgattcg cattgacctg aaccgttgct
    19801 gcttgatagg ttggaaaatt tgaaacattt cccttaccat caacgatctg cccatttttg
    19861 atcgagtacc ccgtgaatgg aatatcattt gctaactgta taccgattga aaaagtactc
    19921 atacctcctc cggcaggaag ccctccccat gttgagggtg taaaagaata acttttatcc
    19981 gcattgggtg taactgaatc gatcgtattt ccagcggtag ccacccccat atagatactt
    20041 tttgggaatg tgatagtagt accatttact gtggagccaa taaacagatt atggatatct
    20101 ggacggttat caataaaagc gattgctttg gttacgcctt gtaacgcagt tttatccgca
    20161 ctcatcgatc cgttaaccgc aacaatgact ccatctttgg catatatcac tgtatatcca
    20221 tctggtatgg tatgtccgtt gaaattcgca gtggccgcaa cagtactaaa tgtctggctt
    20281 ctacctgttt ctgaataagc tttctcccct gctgcttttg ctgctgtgat ttctttttct
    20341 gtcggatcaa tcagttgagt cgaggatgtg gttgaggttg tctcgttgtt gctagactga
    20401 ttggtgcttt ctatggactg tatttcagaa tagggtggac tatttgtctc ctctgtcgtt
    20461 tggttagcaa aatttgacgt ttctgtataa acgtttacat aaatacttga taagctccct
    20521 aagaaagtaa aaatcaagat gatcgaaatc ataatgggat gtaagtattt tcttttagat
    20581 acagctttga gtctagaaac aaaatgtttt tgacatatac gttggatttt attttgagtt
    20641 ttcttaatca ttttcatccc tcctctcaat aaaaaatcat caaataatcc tttacacgtt
    20701 attctaaatt taatttttat tattttattg atgtatagaa agtttggggc tttttttctc
    20761 tttttattct aaggttattt tagtcttgtc ctttaataca aagattttat ttttaaataa
    20821 aaaatactct ttttttacga ttattgaaaa aaaaagataa aacttgatta attgaagtat
    20881 tgcttaaatt tggaagctat gagggaagaa taaaagagtt aggacgggat tatctactat
    20941 ttttctttgt tttaaatatt tgttatcttt gaacataaaa gagaaatgtg tcgactgcag
    21001 tgttgtgtcc ctcatcacga ataaacggtg ttcaattagc tgatcttgta ttcacttaaa
    21061 aaatggtaaa gtacgagttg ctattcccca ttttcaattt tctaacgctc aggacaaaat
    21121 actgttttca caacctcatt ggaataaaag catatatatt gtttcaagaa cgaaatcttg
    21181 ttatcttttt aaagggcatc tgtccaaaat ctaattttag gaggtacgtt ttggtattta
    21241 ttcgtacaaa taaaagagaa ttatctttat tcatgtaacg catccaattg attttttcat
    21301 gtacgctcct ggaacgattt tactgacgat tgcttattta actgcgaatc gctcattagc
    21361 ttttatcata gctatccata ttttgaataa cgttcttggc tttgtattat agatacagat
    21421 gacaaaaaaa gttcaagtat gcggtgtaga accaatatcg catacttgaa cttttttcaa
    21481 tgagtataaa ttaaaacttc taattttcaa ttcccatcat ctgatatgcc cgaccgtctg
    21541 aacttctttt gaaaaagcca aatgtgatta gataacgtct ttgttcgaca taatcgccac
    21601 tagaaaatac gttcagcagt atcgaattaa cctctgtttc tgtataagtc actccatggt
    21661 caaagcgttt caagacttct ttcaatagga tgtatttctt ttttgttgaa cttggtactt
    21721 gttttacttt tccatcattc atgaatgtgt ctaaaacttt catcttttct tgctccgtat
    21781 aagtataaac catcttcttt ctccttttga ttctaagaat actccattta tgttagaata
    21841 ttctggattt tttattaaag cggttactct taagaaagat attatcatag tgctcttttt
    21901 tttactacta cttataggta gatttattct aatccattat tctcccctac caataattaa
    21961 agaaaaaact ggatcagggt gttcaaatta catgctaaga aaaaagaacc cttgaatacc
    22021 aagggttctt tctcagtctg ataacaatga ttaacgacgg atttctttga tacttgtgaa
    22081 aagtcgttaa agtattgata taaaaggatt tttattacct ctgactacat attgactaat
    22141 ttgttaaaaa atcgtttttc aagaatcctg taataccagt gctttcaccg tttttttaac
    22201 tctctaaaga actaaaagca atcagaggtt ttaccttttc atttgacgtt aagtggcgat
    22261 aaattttcga agttgtttgg tcagtatggc ctaagcgatc tttcaagact tcccagctac
    22321 agtgttccgc tactaagctt gccattgtat gacgagctaa atgagtatga aatggcttat
    22381 taattcctga gcgattcacg caccgtttga cgtgatcatt tacatactct cgacgataag
    22441 gaacaccacg tttactagaa tcaagataca caaacaaata tgtttcttct ataataaacc
    22501 tgtaatgttg cctcatctca cccaaagcct taatcaactg ttgtagcttt tccataacta
    22561 gaggagttac tagaactcgt ctagcagaac taactgtctt agtagtttca attcgaaaat
    22621 ccttactctt acctttaact gccgtttgct tatagaacag aatttcattt cttttgaaat
    22681 ctatatctcc aggctgtaga gcaagcgcct cgctgactcg gcagccagtg taaaatagaa
    22741 catggaaaag tacagacatc ggtaattcct catgaacaat gccataattc aaaaaagttc
    22801 tcaattcatc gacagtaaga aacttgtcct caatattatt tatggcttct tttaaatcag
    22861 ttaccgttaa ctgatatttt ggcattctca cagacttcat tggattttca cttatcaaat
    22921 tctttctttg acaatattta aaaatcatat ttagaacttg aataagtgat tgtaaactat
    22981 ttttggcata gccaacattt gttccattaa cgccacattc ctttaaggta aaaagcaatt
    23041 cttgaaggca ctctggtgtc aaatcagaca actttttatc accaatccat cgaacaataa
    23101 tttttaattg ttcctctcgc ttatcatagg ttatttcctt ttgttcattt aacgaacggt
    23161 aatattggaa ccattgctca cctacaacac taaacttgtc attgcttgca acaatttttt
    23221 tcttaagcct ttgttgtttc tccatttcca gataagcgag ttgcgcctca cgttttgaat
    23281 taaagccacg tcttgttgtt cgatatgctt gaaagtacca tgtgatccta ccagtagaac
    23341 tgatttgctt cttgaatcga tctgtttttt ccatttattg attcctccta ttttgaagga
    23401 acccaacaag cagaaaccct ttggaaatct ttctatctat gtcatcattg taccgcatct
    23461 tagaaagaaa atgtaactta taaagatcga agtagtcgca atttttctcg atagtcattc
    23521 gaatcactct ttctcatcca ttctaaaaca tcatcacgaa aatagtgtcc tcgaatcccg
    23581 taccaacagg gaggaaactc accaagtttt actaaacggt tcagtgtact atcacttatc
    23641 tgaaatagtc gctttaaatc agtatttgtt aataccagag gtaaatgact gactgcaagc
    23701 aatttttccg tgtaatcttc cagttttttt tgtaaaattg aatagaactc tatttccaga
    23761 gaattaacat ttgttttaac ttccaactcc atcacccttc tctctcaaaa tcaaaacgac
    23821 caccacggta ccccttaaaa ttgtaaaaaa acactccagt actccatcaa aacggactag
    23881 aaaagaggaa tttttaatat gaaacaagga attgcagaca taaaaataat taaagaaatt
    23941 cttgaaaaat ctacggctaa tgcaatagca ttcggaacag gaatcaactt gagtacagta
    24001 aaaaaactta aaagtggaga gagagctgaa gaaaaattaa atcttgccga tgctattaaa
    24061 atcacagaat ttggcatgaa aaatatgcca accaaaatcg aaatatggaa atcctagctc
    24121 ttccaaagcc atttgtcgtt ggtaccaaac actgccgaga cttttcccaa taatatcgcc
    24181 aatttcacga tcattaagac cgatgaagaa ggacataaaa atgatttctt gtctttgact
    24241 aggtaatgct gcaatcgcat ttgccaactc ctcattggaa ataaagattt tcataccaag
    24301 gacaataaaa gtaccactac tattcaaatt tggttccaaa taatagaact ctgtacctaa
    24361 ccctttttgt tccaatattt ccattgatat ttgccgttgg cgaaatctag cgtacttctt
    24421 ttgaatattt aacgcctcat tgcagactac tttcttacaa aaactgtcaa acgtatgttg
    24481 aatgtgggtt tcctctgagt cgcccataat attctccccc tcaaaaaatt cattcaactc
    24541 gttttacttc tatccctttc cattattact acaatctagg a
//
LOCUS       DACEEZ010000034        24523 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20313_2017
            SAMN12639966-rid9879223.denovo.034, whole genome shotgun sequence.
ACCESSION   DACEEZ010000034 DACEEZ010000000
VERSION     DACEEZ010000034.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639966
            Sequence Read Archive: SRR10023158
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 24523)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24523)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741683.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741683.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:44:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,016
            CDSs (total)                      :: 2,962
            Genes (coding)                    :: 2,889
            CDSs (with protein)               :: 2,889
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 36 of 73
            Pseudo Genes (incomplete)         :: 29 of 73
            Pseudo Genes (internal stop)      :: 18 of 73
            Pseudo Genes (multiple problems)  :: 8 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24523
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639966-rid9879223.denovo.034"
                     /isolate="E20313_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(366..1484)
                     /locus_tag="IWO89_001427"
     CDS             complement(366..1484)
                     /locus_tag="IWO89_001427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0456184.1"
                     /translation="MAKKDKELLTQLTSEGAKGPFVTIMLNTHVAHQDVEKDQIKFKN
                     FAKEAKKRFEKKYSEHDWSPFQEKIENLLGDPSFWRSATTSVAVIITADQTYIHRLSI
                     PVDDQYYVSDLPYLLAIIKNGQFNYSYYLLGLNRDSMKLYEVSNKQVKEVSLPEGAPV
                     DVVTALGSELTGGGLNFSSNGGGGARDGVTFHGVNPKDEEVEIDWVNYYQAVDTYLKD
                     SFENPEQWPIYLFALPENQTMFKKIAKNPYYCADVAISASPAQLSFRDIEEGAKKLSS
                     ELAAQESKEYNKLLDKKFIDQLVDIVPAAKEGKVSHLFIATSNLADGFGEDPETEYDR
                     RQVLNALADDVIINGGKVFILDQKDAPDEKSLTAILRY"
     gene            1955..3262
                     /locus_tag="IWO89_001428"
     CDS             1955..3262
                     /locus_tag="IWO89_001428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase"
                     /protein_id="HAQ0456185.1"
                     /translation="MSIKKTRLVLLLASLILCLASFLPAVAVQAEDTFKVNAKAAFAV
                     DAQSGKILYDQDGEKTMGIASITKIIGLYIVLDQVKEGKLSWGDKVSISDYAENLSIT
                     PDLSNVPLHKENTYTVKELFDSAIIQSANASMVALAEKISGSEAKFTERMKEQLKDWG
                     IKDATIVNASGLNNSYLGENRPEGTGENDENQMSAQDVAIVARHLILDFPEILDVSST
                     TTQMFGENTQSPVEMVNWNWMLPGFVNYKEGVDGLKTGTTELAGACFVGTITKNDQRI
                     ITVVLNADGHAENPSARFNETDRLMDYCYDNWSEKELGKANASIPSLKTIDVKDGKES
                     TVPVVLKSPVKVWVRNDMDTGKLTITPTLDKKQVTDNEIQAPMDKGTKIGTATITLAD
                     DKLGYLEDNNAPSTEIITDKTVEKANIFVIGWRRVAEFFGNLF"
     gene            3381..4580
                     /locus_tag="IWO89_001429"
     CDS             3381..4580
                     /locus_tag="IWO89_001429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcF family protein"
                     /protein_id="HAQ0456186.1"
                     /translation="MNLTRDLIFLVLYLIGVFSIVYWRKQKKDPSLFFYGSTWTFTVI
                     LTYFVVLEIFYSTFYFLIPLLFFSIFAFSYFTEKRRLLNGLLFNVLLISFGIYLFVLL
                     YETQNIFLGGLIALITIPLLLVLLFGIYGLIVFLFWNGVTVLRRESHSLANLLTLILA
                     IFLTLFLVFDFFLLKYLPQWINALFFCVPLILIYLFIVFYNFLTVSFLYQFNRPRYNQ
                     DFIVVLGAGLINGETVSPLLAKRINKAIAFYRAQSRATLNPPILLMSGGQGADEKVPE
                     AIAMKQYAMEQGIPERDILVETNSTTTLENMLYSKEIMDQQMKGPYRAIFSSNNYHIF
                     RAGLYARQAKLKANGIGAKTAFYYLPNAFLREYLAILMMNKKRHMIICGLLFAGSAFL
                     SLITLLF"
     gene            complement(4607..5875)
                     /locus_tag="IWO89_001430"
     CDS             complement(4607..5875)
                     /locus_tag="IWO89_001430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="voltage-gated chloride channel family protein"
                     /protein_id="HAQ0456187.1"
                     /translation="MEKQLPSPVRILVYMLKWLVITAILGIFMGSLSAFFLNSLTVVT
                     DIRLSHPWLLCLLPVSGALFAYLYAYHGGLSSRGNNLVIDQGNGGEEKIPLRLIPLTL
                     FGTITTHLFGGSVGREGTAVQMGGALADNTARLFRLDKSEREILIISGISAGFSSVFG
                     TPLAGTLFGLEVLAIGKVRTEAIFPSFFAALFANFMTESYGVSHLHYQMGVIPEWSML
                     LFFKVFVAGIAFGLIGWVFSRSIVFLKARYAQWIPHPVCRNTIGGAVVVAAALILQTQ
                     RYLGLSLPLLQESFNGQAHVYDFIGKLFFTVLSLGAGYQGGEVTPLFEIGATLGAALA
                     PLLHVSVPFLAGLGFIGVFSGATNTPIACFVMGIELFGSQAAVYFFMICLISFMCSGN
                     SGIYSAQQVAVKKGILFLPIVKRMKEKNKV"
     gene            complement(6038..6684)
                     /locus_tag="IWO89_001431"
                     /pseudo
     CDS             complement(6038..6684)
                     /locus_tag="IWO89_001431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010728800.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="FusB/FusC family EF-G-binding protein"
     gene            complement(7092..7595)
                     /locus_tag="IWO89_001432"
     CDS             complement(7092..7595)
                     /locus_tag="IWO89_001432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HAQ0456188.1"
                     /translation="MTEKWREDEEYMSYVQDLLETEAVKKLANYTQHVHSTRLEHSIS
                     VSYYSYLLAKKWGGNAKATARAGLLHDLFYYDWRTTKFDEGTHAYIHPRIAVKNAEKI
                     TDLSDLERDIILKHMWGATIAPPKYKEGYIVTFVDKYCAVKEAAQPMSASMRKRWQRY
                     FGKESSI"
     gene            complement(7718..8482)
                     /locus_tag="IWO89_001433"
     CDS             complement(7718..8482)
                     /locus_tag="IWO89_001433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HAQ0456189.1"
                     /translation="MKEIQSVKNPRIKELKKLHKKKYREEKAEYLLEGFHLIEEAASS
                     DAEIKELFVTQRGMKEWGDWLAVNKEISTFLVSDEVMTAMSDLPTPQGILAVMTLTSE
                     DTPDFSGGWLLLDNVQDPGNVGTMIRTADAFGMAGVLLGKGTADIYSTKVLRSMQGSN
                     YHLPVLQKDLAEVIPVMKGKGFTVYGTELNEEAHALDKVEKTEDFAIIMGNEGQGVSQ
                     EILSSTDQNVYIPMKGNAESLNVGVAAGIVLYHFSL"
     gene            8589..8864
                     /locus_tag="IWO89_001434"
     CDS             8589..8864
                     /locus_tag="IWO89_001434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylphosphatase"
                     /protein_id="HAQ0456190.1"
                     /translation="MRKVKMNVQGRVQGVGFRYMTKMVADQLGVTGTVKNEDDGSVTI
                     EAIGNDDIIQKFIEEVKKSPSPSGRVQYVDVQEDPMIEERKKFDVVG"
     gene            9580..10533
                     /gene="yidC"
                     /locus_tag="IWO89_001435"
     CDS             9580..10533
                     /gene="yidC"
                     /locus_tag="IWO89_001435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HAQ0456191.1"
                     /translation="MNKWKNWLLGSGLVSILLFLSGCVRMDSNGNPDTSGIVYRVLVH
                     PMGQAITYLVENFNWSYGWAVIAMTVIVRIIILPLGISQSKKTMIQSEKMQALKPQVE
                     AAQQKLKAATTREEQMAAQAEMQQVYRENGLSMTGGIGCLPLLIQMPIFSALYFTARY
                     TEGIRESSFYGIDLGSPSMVLVAIAGGAYLLQGYISTIGIPEEQKKTMRTMLIVSPAM
                     IVFMSISAPAGVTLYWVVGGVFSCLQTFITNVIMKPRLKAQVAEELKQNPPKQVVTPR
                     KDVTESAAKPADNKQATKQIKQPKNSTNNKGRNAGRQQKRK"
     gene            10655..11864
                     /locus_tag="IWO89_001436"
                     /pseudo
     CDS             10655..11864
                     /locus_tag="IWO89_001436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017466553.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1182 family transposase"
     gene            complement(12174..12395)
                     /locus_tag="IWO89_001437"
     CDS             complement(12174..12395)
                     /locus_tag="IWO89_001437"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HAQ0456192.1"
                     /translation="MFKNYTINQKILPLDMEQYISETDVAFAVNALVEEKIKVDLYVS
                     SKFTNVKVVGADLFVVNVRKQKVSETDKF"
     gene            complement(12556..13077)
                     /gene="tnpA"
                     /locus_tag="IWO89_001438"
                     /pseudo
     CDS             complement(12556..13077)
                     /gene="tnpA"
                     /locus_tag="IWO89_001438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631987.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
     gene            complement(13168..13534)
                     /locus_tag="IWO89_001439"
                     /pseudo
     CDS             complement(13168..13534)
                     /locus_tag="IWO89_001439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875519.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIA"
     gene            complement(13548..13833)
                     /locus_tag="IWO89_001440"
                     /pseudo
     CDS             complement(13548..13833)
                     /locus_tag="IWO89_001440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311319.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
     gene            complement(13839..14033)
                     /locus_tag="IWO89_001441"
     CDS             complement(13839..14033)
                     /locus_tag="IWO89_001441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0456193.1"
                     /translation="MAETRPKGYPKLKEYTPSRFMLSECHYDKARADRAVNFIGQLRH
                     TKGKWAGNRFWLLPWQEQII"
     gene            complement(14033..14509)
                     /locus_tag="IWO89_001442"
                     /pseudo
     CDS             complement(14033..14509)
                     /locus_tag="IWO89_001442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004070864.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase small subunit P27 family"
     gene            complement(14977..15330)
                     /locus_tag="IWO89_001443"
     CDS             complement(14977..15330)
                     /locus_tag="IWO89_001443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0456194.1"
                     /translation="MAMSEKAKKYLKEIKGAKTIPELKDVEIAIKRDGILAWAEFTKL
                     NEAVEEKKVALRKKKQETSLQEILFWAYKKESDKLLKMMDEGADKDAIQMQVQRYDSI
                     GQIIAEADLEDEYEV"
     gene            complement(15458..15997)
                     /locus_tag="IWO89_001444"
     CDS             complement(15458..15997)
                     /locus_tag="IWO89_001444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0456195.1"
                     /translation="MQLLLYRIGDSTKIRFLINVRFRVIYSTVLPKGIPQRQKWMIHM
                     EEIMQMILGNAKFQLGQVVSTPAVLETFNPLQTANGIPEALSNLWFRQEISIAEIEDA
                     VLDAKNGKELANSLNRIKLFRKFTLDRELDGMVRLKGTDVWKNISYLKVYTATPPKKP
                     TPPRFQSNGWSLFFSHKSA"
     gene            16027..16218
                     /locus_tag="IWO89_001445"
     CDS             16027..16218
                     /locus_tag="IWO89_001445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0456196.1"
                     /translation="MIIRDDHIYTCDSCHYSFPADEQPERCPDCEKTATRLDTEIETE
                     DYYRVRAEIKAEIKALNAG"
     gene            complement(16260..17126)
                     /locus_tag="IWO89_001446"
     CDS             complement(16260..17126)
                     /locus_tag="IWO89_001446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HAQ0456197.1"
                     /translation="MEELFSNALKSVKSNRVLYTASFFARSSLLYLQEIGQLTALKAH
                     TSRRTGLSSYLCFLVEVGSGTLEYKGQKYTLQVGDMVFIDCLKPYVHESSEDDLWTLR
                     WCHFNGIGMTEVYKKYLERGGQPVFKTSKTDKYADLLRSLHMVAEGQEYVRDMKINVL
                     LSELLMHLMEDSWNPDKVPNFSTKKMELQNIKQYLDDHYTEKITLDQLSAEFFINKQY
                     LAKIFRETYGTTVVGYLNQKRITQAKHLLRFTNLSMEEIATKIGITDANYFSRTFFKV
                     EGVRPSEFRKMW"
     gene            complement(17150..18802)
                     /locus_tag="IWO89_001447"
     CDS             complement(17150..18802)
                     /locus_tag="IWO89_001447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-sugar mutase"
                     /protein_id="HAQ0456198.1"
                     /translation="MNYKVEYERWLNHANADIIEELRSYSEKDIEDSFYKNLSFGTGG
                     LRGTIGAGTNRMNVHTVGKASQGLADYLNKNYEAASVVVGYDSRIKSDVFAKTAADIF
                     AANGIQVHLWPELLPVPTVSFATRYLKADAGVMITASHNPSKYNGYKVYGADGCQITT
                     EAATAILAEIEKLDIFADVKMDGDASKIASIQSEVLTAFIEEVKGQSVLFGEEVDKNV
                     AIVYSPLNGTGLVPVTRTLAEMGYSNVTVVEEQRLPDGNFPTCPYPNPEIKEAMSLGM
                     EYAKKYNADLLLATDPDCDRVGIAVKSAHGDYVLLSGNETGLLLLDYICSQRIKHGKM
                     PADPVMVKTIVTMDLGERIASNYGVRTIDVLTGFKFIGEQIGMLEKAGKVDSYIFGFE
                     ESYGYLSGSYVRDKDGVNAAYLICEMFSYYKTQGISLLDKLNEIYTKYGYCLNTLHSF
                     EFDGAAGFTKMQEIMVEFHKGLDIIGGKKVIETLDYSKGLNGLPKSDVLKFMLEDNCS
                     VVVRPSGTEPKLKTYISVSAENKEAAEAMEAIMAAELAEMFE"
     gene            complement(18807..19709)
                     /locus_tag="IWO89_001448"
     CDS             complement(18807..19709)
                     /locus_tag="IWO89_001448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-6-phosphate isomerase"
                     /protein_id="HAQ0456199.1"
                     /translation="MMNLQKELRCLLLRKLGMLYAPGWTELCCVWSEYQGMTLTEVLQ
                     EHPEFLGTHPQTKGELPILIKFIDAKKDLSVQVHPTDEYAIEKENGQLGKTEMWYVLD
                     ASLIYGLQKITEEAVIREAIAQGTVERYLQKVPVKKNDVFFIEAGTIHAIGAGALIAE
                     IQENSNLTYRLYDYDRVDKNGKKRELHVDKALEVANLKGLSEPKQPLRVLKYRAGVAA
                     ELLCRCKHFEVSRMIVNTERRQTVHYRADSISFRVLLCVNGCGTVRFDNESLDIYKGD
                     CLFVPADSTEISIHGSIQFLDVRG"
     gene            complement(19793..21106)
                     /locus_tag="IWO89_001449"
     CDS             complement(19793..21106)
                     /locus_tag="IWO89_001449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HAQ0456200.1"
                     /translation="MNIVLLSGGSGKRLWPLSNDIRSKQFIKIFKTEDGYESMVQRVY
                     RQIKKVDNDATVTIATSKTQVSAIHNQLGEDVGVSVEPCRRDTFPAIALATAYLVDVM
                     GIDPEESVVVCPVDPYVEDDYFEALKSLSDQADKGEANLVLMGIEPTYPSEKYGYIIP
                     ESAEQTAFVNTFKEKPSEAVAKEYIAQGALWNGGVFVYKLKYVMERAHQLIDFVDYQD
                     LFNKYDTLTKISFDYAVVEHEDKIQVQRFAGMWKDLGTWNTLTEGMEESIIGKGVMNE
                     NCDGVHILNELDVPVLAMGLHDVVICASREGILVSDKEQSSYIKPFVDKFEQQIMFAE
                     KSWGSYKVMDVEAESMTIKVTLNASHSMNYHSHKNRDEVWVVLAGTGKTIVDGYEQVV
                     KAGDVITMSAGCRHTIMADTELKLIEVQLGKDINVKDKQKFELEY"
     gene            complement(21174..22118)
                     /locus_tag="IWO89_001450"
     CDS             complement(21174..22118)
                     /locus_tag="IWO89_001450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019150212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-L-fucose synthase"
                     /protein_id="HAQ0456201.1"
                     /translation="MMEKDAKIYVAGHRGMAGSAIVRELNRQDYNNIITRTHKELDLC
                     RQDAVEAFFAQEKPDYVFLAAAKVGGIIANQNALADFMYENMILEMNVINSAWENGCK
                     KLQFLGSSCIYPRMAPQPMPESCLLTSELEKTNEAYALAKISGLKYCEFLNKQYGTDY
                     ISVMPTNLYGPNDNYHPTHSHVLPALIRRFHEAKEAGLPTVTCWGDGSPLREFLYVDD
                     LANLCVFLMNNYSGDETVNAGTGKELSIKELTEMVAKVIGYEGEILWDTSKPNGTPRK
                     LLDVSKATKLGWTYKTELEDGIRLSYEDFLNNPMRAER"
     gene            complement(22155..23168)
                     /locus_tag="IWO89_001451"
     CDS             complement(22155..23168)
                     /locus_tag="IWO89_001451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0456202.1"
                     /translation="MATLCRCEAKITLVCGANHLDFAKRNLEQELPKELIDRVRYRPI
                     NTDIGLINREGTLLVDTGKLTEICEYFLAKVPSLAKTEAEWLKREAVSAVLCDMPIWA
                     MDACDMAGVPFLYVGNFTWTELYREFLPEHIWKEYAKRYGKIQHTMLYALNNEEMLEF
                     LQNSEKTPTSVVARPIHNDEVQRIKSQHEKPPVFVALGMSARFKQPIDVSHAPYDFIT
                     TLGVPLVGENVTVISNSTPNTQDYIAAADYVITKAGWGTVAECILAQRPMALFARDTV
                     LEDRTTICKLQKLGLAISVTPENLHRVERVIEDLKQLDTSNFDTFYDAASEIADKLIS
                     LMQ"
     gene            complement(23248..24294)
                     /gene="gmd"
                     /locus_tag="IWO89_001452"
     CDS             complement(23248..24294)
                     /gene="gmd"
                     /locus_tag="IWO89_001452"
                     /EC_number="4.2.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose 4,6-dehydratase"
                     /protein_id="HAQ0456203.1"
                     /translation="MKKALITGITGQDGSYLADFLLEKGYEVHGITRRASISNTARID
                     HLMGKITLHDGDLSDSSSLIRIISIVQPDEIYNLAAQSHVQVSFDVPEYSGDVDALGV
                     MRILEACRILGLTKKTKIYQASTSELYGKVEEVPQRETTPFHPYSPYAVAKQYGFWIT
                     KEYREAYGMFAVNGILFNHESERRGENFVTRKITLAAGRIAEGLQDHLELGNMDSLRD
                     WGYAKDYVECMWMILQHETPEDFVIATGEQHTVRDFTEKAFAANGMTIRWEGTGIDEK
                     GYDVETGKMLVCVNPQWFRPTDVDNLWGDPTKAKTVLGWNPQKTSYEELVNIMAKHDR
                     ELAKREAAMKNAVK"
BASE COUNT         6973 a         5553 c         4669 g         7328 t
ORIGIN      
        1 tttaaacggt gttccagaag tagcttcttc ggaaataagc cgaaatttca caaaaatttg
       61 agaaataatt tttgtcaatc tcttcttatt tcttgaagct gaccacttct gtcacaacct
      121 cttccttaaa cggtgttcaa aagcttgcat ctgcggtaat aagcccacca aacaaaaaat
      181 atgaagagct atttttgctt gctgttctta ttacttgatg cagaacagct ttttcacaac
      241 ctctcataca cggtgtttaa tcagctgacc ttcggtatta gctcaaaaaa cggaaaaata
      301 tgtgaaacta tttttccgtt tgtttctcta atactcaggt caaaacactg atttcacaac
      361 ctcttttaat aacgaagaat cgctgtcaga ctcttttcat ctggcgcatc tttttgatcc
      421 aaaataaaca ctttcccgcc attaatgatc acatcatctg ctaatgcatt caacacttga
      481 cgccgatcat attctgtttc aggatcttca ccgaaaccat cagctaagtt tgacgtagcg
      541 ataaataaat gtgacacttt tccttctttc gcagctggaa cgatatcaac taactgatcg
      601 atgaatttct tatctaagag tttattgtat tcttttgatt cttgagctgc gagttcactt
      661 gacagcttct tcgccccttc ttcgatatct ctaaaggata gctgcgcagg agaagcagaa
      721 atcgcaacgt ctgcacaata atagggattt ttagcaattt ttttgaacat cgtctgattt
      781 tctggtaatg caaataaata aatcggccat tgttcaggat tttcgaaaga atccttcaag
      841 tacgtatcaa cagcttggta atagttgacc caatcgatct ctacttcttc atcttttgga
      901 tttacgccat ggaaagttac tccatctcgg gctccgcctc ctccattaga agagaagttc
      961 aaaccgccac ctgtcagttc agatcccaat gcagtaacga catcaaccgg tgccccttct
     1021 ggtagagata cttcttttac ttgtttgttg ctgacctcat acaatttcat ggagtcgcgg
     1081 ttcaatccca ataaataata agaatagttg aactgcccat ttttgataat agccagtaaa
     1141 taaggaagat cacttacata gtattgatcg tctaccggga tactcaaacg atggatatag
     1201 gtctggtcag cagttataat gaccgctaca ctcgtagttg cactacgcca aaacgaagga
     1261 tctcctaaca aattttcaat tttttcttgg aacggggacc agtcatgttc ggaatatttt
     1321 ttctcaaaac gtttctttgc ttctttcgca aaatttttga atttgatctg atctttttcc
     1381 acatcttgat gggcgacatg cgtattcagc atgatcgtga cgaatggtcc tttagcgcct
     1441 tctgaagtca attgtgtcaa taattcttta tccttttttg ccataaacgt acccctccta
     1501 aaaaaatcat tcctttgttt ctatctaaag tctaccacaa cactattttt ccatacaata
     1561 aaacgtatct aaaaaaataa aacggtttct acggttttaa atttagctat ttttttaagt
     1621 tatcttcata atttcctcaa ttatttagcc ccagtcttca actattcttc atgaaatgct
     1681 gataaactaa gaaaaagaat aaaagaggtg gatcttatgc atttgattat gattttcagt
     1741 ttattaagta ttatcagttt atttgctttc agcgcttccc gcttattaga tgcggaaaca
     1801 cagcctattc ctgtaaaagt acgtaaaaac gaccgctaaa cttccataac gcgcgttctt
     1861 atcctaaagt tttttcagaa acattgaaac tctctcccct ctctgataaa attttaattt
     1921 gtagcgaaac aaattaatta caaggagatc ggatatgtcc ataaaaaaaa cacgtctagt
     1981 cctattattg gctagtctca ttctttgcct tgcttctttt ctaccagctg ttgctgtgca
     2041 agctgaagat acgttcaaag taaatgcaaa agcagctttt gctgtagatg cgcaaagcgg
     2101 taagattctt tatgatcaag atggagaaaa aaccatgggg atcgcttcaa tcacaaaaat
     2161 catcggtctt tacatcgtcc ttgatcaagt aaaagaagga aaactgtcat ggggtgataa
     2221 agtttcgatt tcagattatg cagaaaattt gagtatcacc ccagatttat cgaatgttcc
     2281 tttgcataaa gaaaacacct acactgtcaa agaattattc gactctgcaa tcatccagtc
     2341 agccaatgct tcaatggtgg ccttggcaga aaaaatctct ggcagcgaag ctaaattcac
     2401 tgaacgaatg aaagaacaat tgaaagattg gggaatcaaa gatgcaacaa tcgttaatgc
     2461 atcaggattg aacaactctt atcttggcga aaaccgacca gaaggcactg gagaaaacga
     2521 cgaaaatcaa atgtctgctc aagatgtggc catcgttgcc cgccatttga tcttggattt
     2581 cccagaaata ctggatgtat caagtactac gacccaaatg tttggcgaaa atacgcaatc
     2641 accagtggaa atggtcaact ggaactggat gctcccagga ttcgttaatt acaaggaagg
     2701 cgtggacgga ttaaaaactg gtacgactga gcttgcaggt gcttgtttcg ttggtacgat
     2761 cactaaaaac gatcaacgga tcatcactgt cgtattgaat gcggacggcc atgcggaaaa
     2821 cccgagtgcc cgcttcaatg aaacagaccg tttgatggac tactgctatg acaattggtc
     2881 agaaaaagag ctcggaaaag ctaacgcaag tattcctagt ctaaaaacca ttgatgtaaa
     2941 agacggaaaa gaatcaacag ttcctgttgt gcttaaaagc cctgtaaaag tatgggtacg
     3001 aaacgatatg gatactggca aattaacgat cacgccaaca cttgacaaga agcaagtcac
     3061 tgacaatgag atccaagcac ctatggataa aggtacaaaa atcggtaccg ctacgatcac
     3121 cttggcagac gataaattag gctatttaga agacaacaat gcaccatcaa cagagatcat
     3181 tacggataaa acagtggaaa aagcgaatat attcgtgatt ggctggcgac gcgtcgcaga
     3241 atttttcggc aacttgtttt aattgaacaa taagaaaaac atctttccat cggaaagatg
     3301 tttttttgct ttttctagtt taaataacag cttttttcat ctattttcgt tatactgtat
     3361 aagaaaaagg agttgaattt atgaatctaa ccagagatct gatcttttta gttctctatc
     3421 ttattggggt attctctatc gtttattggc gaaaacagaa gaaggatcct tccttgttct
     3481 tttacggcag tacgtggaca tttacggtaa tcttaactta ttttgtcgtg ttggaaattt
     3541 tttattcgac cttttatttt ttgattccat tgttgttctt tagtatcttt gctttttctt
     3601 atttcacaga aaaacgaagg ctactgaatg gtcttttatt caatgtttta ctcatcagtt
     3661 tcggcatcta cttgtttgtc ttgctgtatg aaacgcaaaa tatttttctt ggcggactga
     3721 tcgccttgat cacgattcca ttgctacttg tccttttatt tggtatttat ggattgattg
     3781 tttttctttt ttggaacggg gtcacagttt tacgtagaga atcgcattca ttagctaatc
     3841 ttttaacgtt gatactagcc atttttctta cactgtttct tgtttttgac tttttcttgt
     3901 taaaatattt gccgcaatgg ataaatgctt tgtttttctg cgttcccctg atcttgatct
     3961 atctatttat cgtattctac aactttttga ctgtctcatt cttgtatcag ttcaaccggc
     4021 ctcgatacaa tcaagacttc atcgttgtgt taggcgctgg attgatcaat ggagaaacag
     4081 tttctcctct tctagccaag cgaatcaata aggcaattgc tttttatcgg gctcaaagcc
     4141 gtgctacatt aaatccgccc atcttattga tgtctggcgg gcaaggagca gacgaaaaag
     4201 taccagaggc cattgcgatg aaacaatatg ccatggaaca aggaatccct gaaagagaca
     4261 ttcttgtcga aaccaattcg acaaccactt tggaaaacat gctctattca aaggaaatca
     4321 tggatcagca aatgaaaggg ccataccgcg cgatctttag ttccaacaat taccacattt
     4381 ttagagccgg tctttatgcg cggcaagcca aattgaaagc aaatggtatt ggtgcaaaaa
     4441 cagctttcta ctacttgcct aatgcgtttt taagagaata cctcgccatc ttgatgatga
     4501 acaaaaaaag acatatgatc atttgcggat tgcttttcgc aggctccgca ttcttatcgc
     4561 tgattacttt acttttctaa aaaaacaagg tgtgacaaaa catatgtcac accttgtttt
     4621 tttctttcat tctcttaact attggcaaaa agagtatccc ttttttgacc gctacttgct
     4681 gagcagaata aataccgctg ttgcctgaac acataaagct gatcaagcag atcataaaga
     4741 agtatactgc cgcttgtgaa ccgaacaatt cgatccccat cacaaagcag gcaatcggtg
     4801 tatttgtcgc accagaaaat actccgataa agcccaatcc tgcaagaaaa ggaacagaaa
     4861 catggagcaa aggcgctagt gctgcaccaa gcgttgcacc aatttcaaac aatggtgtca
     4921 cttctccccc ttgatatccc gcgcctaaag acaaaactgt gaagaataac ttcccaataa
     4981 aatcatagac atgtgcctgt ccattaaatg attcttgaag taacggaaga ctcaatccga
     5041 gataacgttg tgtttgcaaa atcaatgctg ctgccacaac tacagctcca cctatcgtat
     5101 ttcgacaaac aggatgagga atccattgtg catatcgcgc ttttaaaaag acgatcgaac
     5161 ggctgaatac ccaaccaatc aaaccaaaag caatcccagc aacaaaaact ttgaagaaca
     5221 gcaacatact ccattcagga atcacaccca tttggtaatg tagatgagaa acaccatagc
     5281 tttctgtcat gaaatttgca aacagtgcag caaagaagct agggaagatc gcttctgttc
     5341 ggactttccc gattgccaac acttctaatc caaataatgt tccagctaaa ggtgtgccaa
     5401 atacagaact aaaacccgca ctgattccac tgataatcaa gatttcccgt tctgatttat
     5461 ccaatcggaa caaacgtgcc gtattatctg ctaacgctcc ccccatttgg actgctgttc
     5521 cttcacggcc aacagatccc ccaaataaat gggtagttat ggttccaaac agtgtaagtg
     5581 gaataagccg caacgggatt ttttcttctc caccatttcc ttgatcgatc actaagttgt
     5641 ttccacgact agaaagtccg ccgtgatacg catagagata agcaaaaagc gcaccactaa
     5701 ctggtagtag acaaaggagc caaggatgtg atagtcggat atctgtcaca actgtcaggc
     5761 tatttaagaa aaaagcagac aagctcccca tgaatatacc gagaatcgct gtgatcacca
     5821 gccacttcag catatacact aaaatgcgta ctggactcgg taattgtttt tccataaaaa
     5881 attcctcctc aataaaaaaa cgcctactaa agaaaggggc tgtccccaga ctttagtagg
     5941 cgtcattagc tatctttcgt tagcatttaa aggcgaacac catcacctat tcacttccat
     6001 taaaagatag catatttttc tgtaaaaaac aataaaatta tcgttctttc tttacacgtt
     6061 cagcaaattc atagaaagga gccaatttcg ttaactgttg attacattgg tcactatcat
     6121 gacaaatata attccccttt ttcgtatacg tgccatcaga acctgctttc gttgtagaaa
     6181 gaaaaagcga aacattcgat gttttctgac agattgcaca gattccctta tttatcgttg
     6241 gtgataaagt accgctaatc ccttttaatt tttcatcttc gtaatagacc atgaattttt
     6301 tctgcgtacc tggatcattc cagccgatgt acgtatgttc ttgcaagtcg atttcattcc
     6361 aagctggttg tttgagtttt ttcacttttc ggaataattt ctcgatttgt ggtttgctag
     6421 gctgcataaa cggctgaacg atttgcttca gctcctccaa tgctctttct gcttgcgctt
     6481 ttgtcagttt tacgtctaaa actgtcgtta aaaatgtatc gattctggca gttcgtgatc
     6541 taatatgtct cgaatctttt cagctgctag tgcctgaact gtttgtaccg tttttggatc
     6601 attgacagat gagtacgcat ttaataactg ttctacttct cttattataa aaaaatattg
     6661 gtatggtttt attgtttgtt ccatgatttt aaagtccttt cttctaacca aagtgcatac
     6721 tttgtagaaa aaagtgatct tctgcaaagt atgtgttacc tgaagatcct ggactctttt
     6781 aaataagata cgttttgcat cttatcatca ccccctttaa tgcttattat tctatgtaca
     6841 tgattcattc gataaataaa agtaaaccgt atcttgaaca gatagtcaac tgcaaacaga
     6901 aaaaaggact gtgacaaaag tcgtgtcaaa gtcccttgtt tcgaatgaat ggtgttcaaa
     6961 agcttgcatc tgcggtaata agccgaaact tcacaaaaat ttgagaagta attttcgtaa
     7021 attccttctt atttcttgaa gctgaccact tctgtcacaa cctcaatatg gtctattatt
     7081 ggttttacct gttaaatcga ggattctttc ccaaagtaac gttgccatct cttacgcata
     7141 cttgcagaca tgggctgagc cgcttctttg actgcgcagt atttgtccac gaatgtcaca
     7201 atatatcctt ctttatactt aggcggtgcg atcgttgcac cccacatgtg cttcaaaatg
     7261 atatctcgct caagatccga caaatcggtg attttttctg cattctttac agcaattctt
     7321 ggatggatat acgcatgtgt cccctcgtca aatttcgtcg tacgccaatc atagtagaac
     7381 agatcatgca aaagtccagc acgcgctgtt gctttagcat tgccacccca tttttttgcg
     7441 agcagataac tatagtaaga aacactgatg gaatgctcca aacgagtgga atgaacatgc
     7501 tgtgtgtaat tcgctaattt tttcactgct tctgtttcta aaagatcttg tacataagac
     7561 atatattctt cgtcttcacg ccatttttct gtcataatta tcgatttgcc acctttttga
     7621 atttcatttt cttctgaaat tgatctaagt ataccaccag tgttcggatt attctagtcg
     7681 ctctagataa aatagctaaa gcttaatagt tccttaatca taaagaaaaa tgataaagaa
     7741 cgatccctgc tgcgactccg acattcaatg attccgcatt tcctttcatt gggatatata
     7801 cgttctgatc agtcgaagac aagatttcct gacttactcc ctgaccttcg tttcccatga
     7861 ttatagcgaa atcctctgtt ttttctactt tgtctaatgc atgtgcttct tcatttaatt
     7921 ctgtaccgta caccgtaaac cctttgcctt tcattacagg gatcacttct gccaaatctt
     7981 tttgtaacac aggcaaatga taattgcttc cctgcatgct tcgcagcacc tttgtactat
     8041 aaatatctgc cgttccttta cctaataaaa caccagccat accaaacgca tctgccgtac
     8101 ggatcatcgt tcccacattt ccaggatcct gtacattatc tagtaaaagc cagcctcctg
     8161 aaaaatctgg cgtatcttca gatgttaacg tcatcactgc taagatccct tgcggtgtag
     8221 gtagatctga catagcggtc atgacctcat ctgaaactag aaaagtagaa atctctttgt
     8281 ttacagccag ccagtctccc cattctttca tacctctctg tgtcacaaaa agttctttta
     8341 tttctgcatc agacgacgca gcttcttcga tcagatggaa cccttccaaa agatattctg
     8401 ccttttcttc tctgtatttc tttttatgta gtttttttaa ttctttgatt cttggatttt
     8461 ttactgattg gatctctttc acattcctca cctcaactga ttatatcatg gaataggtgc
     8521 ttgagcttgt ttcatcctaa aaaaatcggt atcattgatt tataaaacag gaaagaaagg
     8581 tgacgaacat gcgcaaagtt aaaatgaatg tccaaggacg tgtccagggc gtgggatttc
     8641 gctatatgac caagatggtt gctgatcagt taggcgtgac cggaacggta aaaaacgaag
     8701 acgacggctc agtcacaatc gaagcaatcg gaaatgacga catcatccaa aaatttatcg
     8761 aagaagtcaa gaagtctccg agcccaagtg gacgagtgca gtatgtggat gtccaagaag
     8821 atccgatgat tgaggaacga aagaaatttg atgtggtggg ataaggttgt ttttgaagcg
     8881 ctttgcctcg agtattaagg agaaacattt tccaaatagc ttctcatatt ttgaaatgta
     8941 atgacttaat accgagaggc agctgataaa acaccgtcaa taaggttgtg aaaaagctgg
     9001 tctgcatcaa gcattaagga ctggttgtga aaaatagctc tccatatttt tcaacaggct
     9061 ggacttaatg ccgtagatgc aagcttttga acatcgtcga taaggttgcg aaaaagctga
     9121 tctgcatcaa gcattaagga ctggttgtga aaaagtcgct tagttttgag tattaaggaa
     9181 aattttgtga aaatggcttt ccacattttt gcataaattt gacttaatac caagaaactg
     9241 acttttgaac atcgtcgata aggttgtttt tgaggcactt tgcctcaagt attaaagaca
     9301 ggtagtaaaa aagctgatct ataacttgca ataaatttgc ttaacccata ttttccagac
     9361 agaacaaaaa agtttttagt tttttaaata agaaagaaac acttggaatc actcttttct
     9421 ttctaagtat ttctttcttg attttttgtc ttttttcaaa atttttcgct acgaaaaaga
     9481 acgaatattt atatgaaatt tctatgaagt tgattgaatt ccatgcgctt ttttagtata
     9541 gttaagtttg tgtaaaaatt atagaagagg aagtattcta tgaataaatg gaaaaactgg
     9601 ttattgggat ccggacttgt cagtatcctg ctctttttat ctggctgtgt gcggatggat
     9661 tcaaacggaa accctgacac ttcaggaatc gtttatcgag tactcgtcca tcctatgggc
     9721 caagcgatta catatctagt tgagaatttc aattggtctt acggctgggc agttattgca
     9781 atgactgtga tcgttcgtat catcatcttg ccactaggta tcagtcaatc gaaaaaaaca
     9841 atgatccaat ctgaaaaaat gcaagcgtta aaaccacaag ttgaagcagc acaacaaaag
     9901 ctaaaagctg caacaacacg tgaagaacaa atggcagcac aagcagaaat gcagcaagta
     9961 tatcgcgaaa atggattgag tatgaccggc ggaatcggct gtctgcctct attgatccaa
    10021 atgccgatct tctctgcctt gtattttacg gctcgctaca cagaaggtat ccgtgaatca
    10081 tctttttacg gcatcgatct aggctcacca agtatggtgc ttgtggccat cgcagggggc
    10141 gcttatctgc ttcaaggata tatttcaaca atcggtattc ctgaagaaca gaaaaagacg
    10201 atgcgtacga tgctgatcgt ctctcctgct atgatcgtct tcatgtcgat cagtgcgcca
    10261 gccggtgtta cactttactg ggtagtcggt ggtgtgttca gctgtctgca aacattcatc
    10321 acaaacgtta taatgaaacc gcgtttgaaa gcacaagtgg cagaagagtt gaaacaaaac
    10381 ccgccaaaac aagtcgttac tccaagaaaa gacgtgacag aatcagcagc aaagccagct
    10441 gacaacaaac aagcaacaaa acaaatcaaa caaccaaaaa acagtacaaa caacaaaggc
    10501 cgcaacgcag gaaggcagca gaagcggaaa taaaaatata tgacaaaaaa ctgtagacaa
    10561 ataaaaaaat ttctttgttc tacagttttt ttgttacaat taaagaaaac gttcgcattt
    10621 cgaggaggaa tgtataatga tgtccaaata atcaatgtat agtcgaatgg aagaagacaa
    10681 aatttcgcta gatgcacttg ttcctaaaaa tcatttagtc cgtaaaattg acaaattatt
    10741 gtcatttgat tttatttatc tcattgttga atcaacttac tcgaccattg gtcgccctag
    10801 cgttgatcct gtcgtcttag taaaattagt attcattcaa tatttatttg gaatttgttc
    10861 gatgcgtcag accattaaat aagttgaaac aaacctagct tatcgttggt ttcttgggct
    10921 tagtttaatt gataaagttc ctcatttttc aacttttggt aaaaactatg cacggcgttt
    10981 cagagaaacc gatttgtttg aacagatatt tatcattatt ctcgaataag cagttgatgc
    11041 tagatttatt catgaagaaa ttttgtatat ggattcaact catattaaag cgaatgcgaa
    11101 caaacgtatt tttacaagag atatggcaca taaagaagcc attttatacc aaaaacaatt
    11161 agaagaagaa atcaatgagg caagaattgc cgaaggcaaa cgtccttttg gttcaatcat
    11221 tcgtcaggag gtggtcgaaa gaaaatttag catcgcagat ccagaaagtg gttattatgt
    11281 aaaaacagaa cgagaaaaac agtttgctta ttctgctcat accgttggtg atgaaaatgg
    11341 ttttgtatta gatgtcatga ttacgcctgg aaatctacat gatagccgaa tgctcgtgcc
    11401 acaaatcgaa cgcgttaaat cacgttatgg tgtagctgct aatgctgcgt acaaaacacc
    11461 ttggaacgtc aagtatttaa tcgaacgaaa actacgaccc attctccctt atatcagttt
    11521 cgcacaacta ctcgagatgg ctataaaaaa tatgtatcta aatcttttat ttgtgaaagt
    11581 tgtccattat tgaaaaattg tacagaaagc caaaaacacc aaaaagaaat acatcgacat
    11641 atttgggaag actacttaga tgaagcagaa catttacgcc atacggtgta caatcgtgaa
    11701 aaatataaaa aaagaaaaga aacgattgaa cgcatttttg cagacgcaaa ggaaaaacat
    11761 ggaatgcgct acaccaagta tcgaggcttg gaaaaaatca ggtgcataca atgcttgttt
    11821 ttgtcgcaat gaatctgaaa aaattagcta cttggcagtg ggagcatatg tcgttatatc
    11881 ttttattata caaaaataga caaaagtatc agtctacact cttagtaaag aggaaactga
    11941 tgcttttgtc tacagtctta gagagtggga caatagtttt ttcttcttta cgtcttttcc
    12001 tctccatgta ttttcctaat tttcttaatt taacgccatg agtgccaagc caatgtcaat
    12061 cttggctcct tgtttccttc ataagtggaa atccgtttcc gatagatcga accttctttt
    12121 tcctctaaaa gtttttttac attctgcttt gaaataacgc catgtattat tcactaaaat
    12181 ttgtcggttt cgctcacttt ttgctttcgt acattgacta cgaacaggtc agcaccgacc
    12241 actttcacat tcgtaaattt tgaagacacg tataaatcca cttttatctt ttcttcgact
    12301 aaagcgttga ccgcaaaagc gacatctgtt tcagaaatat attgttccat atctaatggt
    12361 aaaatttttt ggttgatggt ataattttta aacataaagc actcgcttcc tttctgattg
    12421 ggtttggtca ctttaatcct acagggatga gtgctttttg tgtaccttaa aatcagtaaa
    12481 tatccatccg tgaaaacggg tggcttttta acagccctag aagggctcac cactcacagc
    12541 cctatctctc tttaactatt taaatggatc tacatactct tgtttactta tactgtctct
    12601 aattcgatca tctgcttctt gttctcaaat atatatttca attgtttttt gatttaaccc
    12661 aaccgtactc acgtagtatc ctttggtcca aaagctttta ttcccatact tatatttcaa
    12721 atttgcatgt ttttcatgga ttaaggttgc acttcgtcct tttaagtagc ccataaaact
    12781 agaaatacta atctttggcg gtattcttac taacatatga atgtgatcag gcattgcatg
    12841 agcttcaatg atttcaacat ctttcctctc acacaatttt cttaatatct ggccaatact
    12901 tactcttaac tttccataaa tcacttttcg tcgatacttc ggggtaaaga ctatgtgata
    12961 cttacaatcc catctcgtat gtgctaaact tttatcgcta aagctatttc gtccacgcgt
    13021 agaaacgcgc gtttttttgt ttcgtacgct acgccaacga aactgactaa agtcaataaa
    13081 aaaagtgggg caataatcag atttttttct gactattgtc ccactctctt tttattactc
    13141 ttcatcttct actattccac taattaatta gttgacagat acaatgcttt cacatcctgc
    13201 acgaacaaac tcgggctctg aaccaatcac ttgcgagttg ataaattcaa tgaccatagg
    13261 aagattaatc cgacataaag tttaatctgt tgtccttcca ttatcagttt agaaatgata
    13321 ttagtaggtg taccgcctaa aagatcgcat agtaccacaa aatcatttaa cgaatctatg
    13381 acagtaagag cttttgtctg tatgtttcag tgccatcttc tggtaataaa ggtaacgtgt
    13441 agatattctc ttgaggtccc ataatcattt ttgtactttt tttcaattcc tcacaaaatt
    13501 ttccgtgact aatcaatatc aattttctac tcatttttgg ctctccctta tttcgaaatc
    13561 acacacaatg cagataaagc cacacacaag taatacgata aaaatcgctc gagttgggtc
    13621 atatttttct ttcctaacaa ccaataaaca cctcgacgat cccagcgagt aagattttag
    13681 ataatatcat atcgatatag ttttgcatat tcaatgtcac atcgccaatc gttggtacca
    13741 taaaaattta atatcaatca ttgttgcaac taatgctcct accatgaaca ctcttaataa
    13801 agtcgctacg tccgttaata cagttaagcg agattacact agatgatctg ttcctgccaa
    13861 ggcaagagcc agaagcggtt gcctgcccat ttgcctttgg tgtgtcggag ctgtccgata
    13921 aagttcaccg cccgatccgc tctggctttg tcgtagtgac attccgaaag catgaatcgg
    13981 cttggtgtgt attcctttaa tttgggatag ccctttggtc tggtttctgc catcagctac
    14041 cacctcccaa cagagcctcc atcgcatctt ctgctccgac accaccgcca tcaccggcaa
    14101 tgatacggct tctggacgaa ggcgtcagac cgaactgctc ggaaaatcgg ttcataattt
    14161 tcagataggt ctgggcaata aacacctgtg gtacctgctg ccaatagccg gatagggttt
    14221 tcacgatgat accgtgctgg gtaatgaatt cctcggcttc cttccagcgg gcgtaggctt
    14281 ggcactaacc ggcaaaggca gccatatcca catctgtcaa gattccgatg gcctccatct
    14341 gctttgccag acgcttccat tccttctttg cctctggttc cagccacttc ggacaggtcg
    14401 gtgccttctt ttccggcgtt gactcttttg cattgagtgg gcgattgccc ggatttccct
    14461 ccagcttctt catggctgtc ggggttgatt ttcttcctct ggttgccaca gtcgtcacct
    14521 cccttcaatg tatttctgcc agtagacaga gaacagagcc aagtcgaaac ctgactctct
    14581 tctctatctg ctaacttgta tttcagtcca ctcttctgat gaatcgcacc cgcaggattt
    14641 tcaagatgcc gcaggcaaat tccaagaagt ttctgaccgc atcataacca tcgtagccgt
    14701 gtagccacat ccttccacct cgccgccgtc ctcgtcgctg atataaatga ttctgtattc
    14761 gctcatctgt tgttttctcc agtcttttgt ttttttgtag tacatatatc actctaaaag
    14821 tgattattat caagtcgttt gaaagccata atgtacacaa tcattcgact ggtttttggt
    14881 tcataattga ccgccaaagt gagcaaaaga aagggacctc tgtctgtttc agaagtccct
    14941 ttcggtattt ggttcttagt ggttcaggct gtattctcaa acctcatact catcttcgag
    15001 gtctgcctct gcaatgatct ggccgatgct gtcgtaacgc tggacctgca tctggatggc
    15061 atccttgtct gctccctcgt ccatcatctt cagcagcttg tctgattcct ttttgtaggc
    15121 ccagaaaagg atctcctgaa ggctggtctc ctgctttttc ttgcgaaggg cgaccttctt
    15181 ctcttccacc gcctcgttca acttggtgaa ctctgcccaa gcaaggatac cgtctcgctt
    15241 gattgcgatc tccacatcct tcagctccgg gatggtcttt gcacccttga tctccttcaa
    15301 atatttcttt gccttttcgc tcattgccat tagcgtttcc tccttgtttt tgggattttc
    15361 ccctttggtt gtgtgtatat tgccatagtc tctcgactat atcaagtcaa tccacaggca
    15421 tatactacac aatcatttgg agctgtattt gtatacatta tgccgattta tgagagaaga
    15481 aaaggctcca gccgttcgac tggaaccttg ggggggtcgg tttcttaggc ggcgtggcgg
    15541 tgtagacctt gaggtagctg atgttcttcc agacgtccgt gcccttcagt ctgaccatgc
    15601 cgtccagctc tcggtcgagg gtgaacttgc ggaaaagctt gatgcggttg aggctgtttg
    15661 ctagctcctt gccgttcttg gcatccagaa ctgcgtcctc gatctccgca atactaattt
    15721 cctgtctaaa ccaaaggttg ctcaaggctt cgggaatgcc gtttgcggtc tgcagggggt
    15781 tgaaagtttc caatacagca ggggtcgaaa ccacctgtcc gagctggaat tttgcgttcc
    15841 ctaaaatcat ctgcataatt tcttccatgt gaatcatcca tttctgccgt tgtggtattc
    15901 cctttggcag tacagtacta tatatcactc tgaatcgtac atttatcaag aagcggattt
    15961 ttgtggaatc accaattcga tataatagta attgcatgaa aaatctaagc actggattgg
    16021 taggtgatga ttatacgtga cgatcacatt tacacctgtg attcctgcca ctacagcttc
    16081 cctgccgacg aacagccaga gcgttgtcct gactgcgaaa agacagcaac tcggctggat
    16141 accgaaatcg aaactgaaga ttactatcgt gttcgtgctg aaatcaaagc tgagattaaa
    16201 gctttgaatg cgggttaacg acaggtactg accggcggct caacaccgcc ggctttttat
    16261 taccacatct tgcggaattc gctgggacga acaccttcca ccttgaaaaa agttctggaa
    16321 aaatagtttg catctgttat gccaattttt gttgcaatct cttccatact tagatttgta
    16381 aagcgtagca agtgcttggc ctgtgtgatt cgtttctgat tcagataacc gacaacagtt
    16441 gtaccgtaag tttcacgaaa aatcttagct aagtactgtt tattgataaa aaactctgca
    16501 ctcaactgat ccagcgtaat cttctccgtg taatggtcat ccagatattg ttttatgttc
    16561 tgcaattcca ttttttttgt agagaaattc ggcactttgt caggattcca agaatcttcc
    16621 atcaaatgca tcagcagttc actgagtaga acattgattt tcatatcccg gacatactcc
    16681 tgtccctctg ccaccatatg aaggctcctc agcaagtcag catacttatc tgtttttgat
    16741 gttttaaaga ccggttgacc acctcgttca aggtattttt tgtagacttc cgtcatacca
    16801 atgccattaa aatggcacca ccgcaaagtc cacaaatcat cttctgatga ctcatggaca
    16861 tacggcttca aacagtcaat aaacaccata tctcctactt gaagcgtata tttctgcccc
    16921 ttatactcca atgtgcctga accaacttct accaggaaac aaaggtaaga tgacaatcct
    16981 gtacgcctag atgtatgtgc cttcagagca gtcagctgtc caatttcctg caaatacaaa
    17041 agagaggatc ttgcaaagaa cgatgccgtg tacagcacac gatttgactt caccgatttc
    17101 aaagcgttag aaaacagttc ctccattaca agatttctcc tttcttttct tactcgaaca
    17161 tttccgccag ttcagcagcc ataatagctt ccatagcttc ggcggcttcc ttgttctctg
    17221 cgctgacgct gatataggtc ttcagcttcg gctcagtacc agaaggacga accacaacag
    17281 agcagttatc ttccagcata aacttcaaaa catcagactt cggcagacca ttcaggccct
    17341 tactgtagtc taaagtttca ataaccttct taccaccaat tatatcgaga cccttgtgga
    17401 attcaaccat aatttcctgc atcttggtaa aacctgccgc accgtcaaac tcgaaggaat
    17461 gcagggtgtt caaacaatag ccatactttg tataaatctc gttcagctta tccagcaggc
    17521 tgataccctg agtcttgtag taagagaaca tttcacagat tagataagca gcgttaacac
    17581 cgtccttgtc acggacatag ctaccggaca gatagccgta agattcctca aagccgaaga
    17641 tatagctgtc aaccttacca gccttttcca acataccaat ctgctcaccg ataaacttaa
    17701 agccggtcag cacatcgatg gtacgaacac catagttgga tgcgatacgt tcacccaaat
    17761 ccatggtgac gatggtcttg accatcacag ggtcagcagg catttttcca tgcttaattc
    17821 tctgagaaca gatgtagtcc agaagtagca gaccagtctc attaccagac aacagaacat
    17881 agtcaccatg tgcgctctta actgcgatac caacacggtc gcagtcaggg tcagtggcca
    17941 acagcagatc cgcattgtac ttcttggcat actccatacc cagagacata gcttccttaa
    18001 tttcagggtt cggataagga caagttggga aattaccgtc cggcagacgc tgttcttcca
    18061 caacggtcac attgctgtat cccatctctg ccagggtgcg ggtaacaggc accagaccgg
    18121 taccattcag aggagaatac acgatagcaa cattcttgtc cacttcttcg ccaaacagga
    18181 cggactgccc cttcacttct tcgatgaaag cagtcagaac ttccgactga atggatgcaa
    18241 tcttagaagc atcgccatcc atctttacat cagcaaagat gtccagcttt tcgatttctg
    18301 ccagaattgc agtagcagcc tcggtagtaa tctgacagcc gtctgcaccg tataccttgt
    18361 agccgttata tttagaaggg ttatgagatg ccgtaatcat aacaccggca tctgccttca
    18421 gataacgagt cgcaaaagat actgtaggaa caggaagcag ttctggccac aagtgcacct
    18481 gaataccatt tgcagcaaag atgtctgcgg cagtctttgc aaacacatca gatttgatac
    18541 gggaatcata gccaaccacc acggaagcgg cttcatagtt cttattcaga tagtctgcca
    18601 gaccttggct tgccttacct acggtgtgta cgttcatgcg attagtgcct gcaccgatgg
    18661 taccacgcag accaccagta ccgaaagaca aatttttata gaaagagtcc tcaatgtcct
    18721 tctcagaata ggaacgcagt tcttctataa tatctgcatt ggcatgattc agccaacgct
    18781 catattctac tttataattc atattattat cctctcacat ccagaaactg aatcgacccg
    18841 tgaatgctga tttcagtaga atcggccggc acaaaaaggc agtccccctt atagatgtcc
    18901 aacgattcat tatcaaatcg aacggtacca cagccattga cacacagcaa cacacggaag
    18961 ctgattgaat ccgcacgata gtgtaccgtc tgtcttctct cagtgttgac aatcatacga
    19021 gagacctcga aatgcttgca tctgcaaagc aattccgcag ctacaccagc acgatatttt
    19081 agaacacgaa gcggctgttt cggttctgac aaccctttca agttcgctac ttccagcgct
    19141 ttgtctacat gaagttctct ctttttacca tttttgtcaa cacggtcata atcgtagaga
    19201 cgataagtca gattggaatt ttcctgaatt tctgcaatta gtgcacccgc accaatcgca
    19261 tgaatcgttc ccgcctcaat aaagaacaca tcattctttt tgaccggaac cttctgaaga
    19321 taacgctcaa cagttccctg agcaatcgct tcacggatca cagcttcttc tgttattttc
    19381 tgcagcccat agattaagct ggcatccagt acataccaca tctctgtctt tccaagctgc
    19441 ccgttttctt tttcaattgc atattcatct gtgggatgta cctgaacaga caaatccttt
    19501 ttcgcatcaa tgaatttgat caggatcggc aattctcctt tggtctgcgg atgcgttccc
    19561 agaaactctg ggtgttcttg caacacctcc gtcaaggtca tcccctgata ctctgaccag
    19621 acacaacaaa gctcggtcca tccgggtgcg tagagcattc caagtttccg caagaggaga
    19681 catctcaatt ccttttgcaa attcatcatt caggcggttc ccccccacag ataatccttt
    19741 gcggaaggct tcaataaaaa cggtttctgt ataacgagcc ctcctccgtt ttttaatact
    19801 ctaactcaaa tttctgttta tctttaacat tgatatcctt gccaagctgt acttcaatca
    19861 gcttcagctc agtatctgcc ataatggtat gccgacagcc agcactcatg gtgatcacat
    19921 cgccagcctt aacaacctgc tcatatccat ccacgatggt cttaccagta ccggccagaa
    19981 ccacccacac ctcgtcacgg ttcttgtggc tgtgataatt catggagtga ctagcattca
    20041 gagtaacctt aatggtcatg ctctctgctt ctacatccat aaccttataa ctaccccaag
    20101 acttctctgc gaacatgatc tgctgctcga acttgtcaac gaaaggctta atatagctgg
    20161 actgttcctt atcggaaacc agaatacctt cccgagatgc acagatgacc acatcatgca
    20221 gacccatagc caacacagga acatccagtt cgttcaggat gtgtacgcca tcgcaattct
    20281 cattcataac acctttaccg ataatggatt cctccatacc ttcggtcaga gtattccaag
    20341 tccccagatc cttccacata ccagcaaaac gctgtacctg aatcttgtcc tcatgctcaa
    20401 caacggcata gtcgaaggaa atcttagtca gagtatcgta tttgttgaac agatcctgat
    20461 aatctacaaa gtcaatcagc tgatgtgcac gctccatgac atacttcagc ttgtagacaa
    20521 acacaccacc gttccacagt gcaccctgtg cgatatattc ctttgccaca gcctcagaag
    20581 gtttttcctt gaaggtattt acgaaagcag tctgctctgc gctctcagga atgatgtagc
    20641 cgtacttctc ggaaggataa gtcggttcaa tacccatgag caccagatta gcttcgcctt
    20701 tgtcagcctg atcggacaga gatttcaggg cctcaaaata gtcatcttct acgtaagggt
    20761 caaccgggca aacaactacg gactcttctg gatcaatgcc catcacatct accagataag
    20821 cggtagccaa tgcgattgcc gggaaggtat cgcggcgaca aggctctaca gatacaccca
    20881 catcctcacc aagttggtta tggatagcag aaacctgtgt cttagaggtt gcaatcgtaa
    20941 cagttgcgtc gttatctacc ttcttaatct ggcggtagac acgctgaacc atggattcat
    21001 atccatcttc agttttgaaa atcttaatga actgcttgga acggatgtca ttagacagcg
    21061 gccaaaggcg cttaccagaa ccgccagaca gtaaaacaat attcataatg atctctcctt
    21121 tttataagaa agaggctgct gcaatcctca aaaatcacag caacctcggt aaattagcgt
    21181 tcagcacgca tcgggttatt caggaagtcc tcgtaggaca gacggatgcc atcttccaac
    21241 tcagtcttat atgtccatcc cagcttggtt gccttggaaa catccagcag cttacgaggt
    21301 gtaccgttcg gcttgctggt atcccacaga atctcgcctt cgtaaccaat aacctttgct
    21361 accatctcgg tcagttcctt gatagacagt tccttgccag tacctgcatt aacagtctcg
    21421 tcaccgctat agttattcat caggaacaca cacaggtttg ccagatcgtc aacatatagg
    21481 aattcgcgca gaggagaacc atcaccccag caggtaacag taggcagacc tgcttcctta
    21541 gcctcatgga agcgacgaat caaagcgggg agcacatgac tgtgagtagg atgatagttg
    21601 tcgttaggtc catacaggtt agtaggcata acagagatat agtcagtacc gtactgcttg
    21661 tttagaaact cacagtactt caggccagaa atctttgcca gtgcataagc ctcgttggtc
    21721 ttttccagct cacttgtcag cagacagctt tccggcatcg gctgcggtgc catacgcgga
    21781 tagatacagg aagaaccgag gaactgtaac ttcttacagc cattctccca tgcggagttg
    21841 atgacgttca tttccagaat catgttttca tacatgaagt ctgccagagc gttctggttt
    21901 gcgataatac cgccaacctt tgcagcagcg agaaatacat aatccggctt ttcctgtgcg
    21961 aaaaatgctt caacagcgtc ctgacggcac aggtcaagct ccttatgggt gcgggtgata
    22021 atattgttgt agtcctgacg gttcagctcg cgcacgattg cggaaccagc catgccacga
    22081 tgaccggcta cgtagatttt tgcgtctttt tccatcataa taatgcttgc tcctttctaa
    22141 atttagagca tctctcattg catgagagaa atcaatttgt ctgcgatttc gctcgcagca
    22201 tcataaaatg tatcaaaatt tgaggtgtct agctgtttga ggtcctcaat cacccgctct
    22261 acgcggtgga ggttttccgg tgtgacggaa attgccagac ccagtttttg caatttacaa
    22321 atagttgttc tatcttccaa aactgtatcg cgggcgaaca gtgccatcgg acgctgtgcg
    22381 agaatacact ccgcaacagt tccccatcca gcctttgtaa taacgtaatc tgccgccgct
    22441 atatagtcct gtgtattggg ggtgctgtta gaaataacgg ttacgttttc tcccacaagc
    22501 ggaacaccta gcgttgtaat gaaatcatac ggcgcgtgac tgacatcaat cggctgtttg
    22561 aaccgcgcgg acataccgag tgcgacaaat acaggcggtt tttcatgctg tgatttaatg
    22621 cgttgcactt cgtcgttatg aatcggacgc gccacaacgg aagtcggtgt tttttcgctg
    22681 ttttggagaa attccaacat ttcttcgtta tttagcgcat aaagcatggt gtgctgaatt
    22741 ttaccgtaac gcttggcata ttctttccaa atatgttcgg gtaaaaattc gcgatacagc
    22801 tctgtccaag tgaagtttcc tacatacagg aaaggtactc ctgccatatc gcaggcatcc
    22861 attgcccaga ttggcatatc gcaaagcact gcgctgacag cttctcgctt gagccattcg
    22921 gcttctgtct ttgccaaaga aggaaccttt gccaaaaaat attcacagat ctcggtcagc
    22981 ttacccgtgt ccaccagtag ggtgccctca cggttaatca gaccgatatc tgtgtttatt
    23041 ggacgatagc gcacgcggtc tatcagctct ttgggcagtt cctgctccaa attgcgcttt
    23101 gcaaaatcca gatgatttgc gccgcaaacc agtgtaatct tcgcttcgca tcggcagagt
    23161 gttgccataa taatgggcag attccgcgcg atatgtccga agccgtgact ggaaagatag
    23221 aaacagatat gtttattcaa tgtcagctta cttgactgcg ttcttcattg cagcttcgcg
    23281 ctttgccaat tcacggtcat gctttgccat gatgttaacc agttcctcgt aagaggtctt
    23341 ctgcggattc caaccgagaa cggtctttgc cttagtcggg tcgccccaca ggttgtcaac
    23401 gtcagtcgga cggaaccact gtggatttac acaaacgagc atcttgccgg tttcaacatc
    23461 gtagcccttt tcatcaatgc cagtgccttc ccaacgaatg gtcatgccat ttgctgcgaa
    23521 tgccttctcg gtgaagtcac gaacagtgtg ctgctcgccg gtagcgatca cgaagtcctc
    23581 gggagtctca tgctggagaa tcatccacat gcactcaaca tagtcctttg catagcccca
    23641 gtcacgcagg gagtccatgt tgcccagttc cagatggtcc tgcaggcctt ctgcaatgcg
    23701 gccggcagcg agggtgatct tacgggtgac gaaattctca ccgcgacgtt cggattcatg
    23761 gttgaacaga ataccgttga cagcgaacat gccataagct tcacgatatt ccttagtgat
    23821 ccagaagcca tactgctttg caacagcata agggctgtaa ggatgaaacg gagtggtttc
    23881 acgctgagga acttcttcaa ccttgccata cagctcagaa gtagatgcct gataaatctt
    23941 agtcttcttc gtcaggccca gaatacggca agcttccaga atacgcataa cgcccaaagc
    24001 atcaacatcg ccggagtact caggaacatc aaaagaaacc tgaacatggc tctgtgcagc
    24061 gaggttatag atttcatcag gctgaacaat gctgatgata cgaatcagag aagatgagtc
    24121 ggacaggtca ccgtcatgaa gggtgatctt gcccatcaaa tggtcaatgc gagcggtatt
    24181 ggaaatagaa gcacggcgag tgataccgtg aacttcgtag cccttctcta gcaggaaatc
    24241 tgccagatag gaaccgtcct gaccagtaat accagtaatc agcgcttttt tcatgataca
    24301 ttatcctttc ctatgtaagt gataaggcca tataaatggc tcgtaatttc taatactttt
    24361 caagaataca cgataatttt cgatatttca tagaacaatc tacgcacgca ttagcacaat
    24421 attagttttt cgtgaattgg aatggcaaca gtttttttga caaattttat aaggtgcaga
    24481 acttctttcc gtatgctatt ccgattgtcc ttgacaatga gcc
//
LOCUS       DACEEZ010000035        24390 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20313_2017
            SAMN12639966-rid9879223.denovo.035, whole genome shotgun sequence.
ACCESSION   DACEEZ010000035 DACEEZ010000000
VERSION     DACEEZ010000035.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639966
            Sequence Read Archive: SRR10023158
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 24390)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24390)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741683.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741683.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:44:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,016
            CDSs (total)                      :: 2,962
            Genes (coding)                    :: 2,889
            CDSs (with protein)               :: 2,889
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 36 of 73
            Pseudo Genes (incomplete)         :: 29 of 73
            Pseudo Genes (internal stop)      :: 18 of 73
            Pseudo Genes (multiple problems)  :: 8 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24390
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639966-rid9879223.denovo.035"
                     /isolate="E20313_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(75..1442)
                     /gene="nhaC"
                     /locus_tag="IWO89_001453"
     CDS             complement(75..1442)
                     /gene="nhaC"
                     /locus_tag="IWO89_001453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaC"
                     /protein_id="HAQ0456204.1"
                     /translation="MNKNISLKESIAVLIVLLALLGVLIIGFQLSPHIPILAAFMCLL
                     FYGRFKKFTWDEIHDGIIKGITPGIIPILIFLLIGVLVASWILSGTIPTIMVYGFKLV
                     SVRFFLPTAFLVCAIVGVTVGSSFTTISTIGIAFLGIGHLLGFNDAMTTGAVVSGAFL
                     GNNCSPLSDTTNLAAGIGGVNLFEHIAGMRYTDFPAFVLSLIFYTILGHNSHSADLAS
                     INEMIQNMKASFWISPWTLIPLVILFGGAIKKIPAIPTLLAGSTAALVLSFIHEKSLT
                     VGKAADILMNGYVANTPDPKLDELLSRGGIMSMLGSASLILLALALGGLLIKYLIVDT
                     IVQELKEKMDRPSRLVGFTALSCIGINLIVGEQYLSIILPGETFKRSFDDVGLSKTYL
                     TRTLADSGATVNSLVPWGVSGTFIMGTMKVSALHYLPFVFFSILAPVFTILGGFLLNR
                     RRVKN"
     gene            complement(1552..2973)
                     /locus_tag="IWO89_001454"
     CDS             complement(1552..2973)
                     /locus_tag="IWO89_001454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAQ0456205.1"
                     /translation="MSKSEVSKMGLGTFIGLTMALCATVRSIPTLAAVGWTLIFYSIF
                     AVLFFAGPISMVSGELSTMLPQEGGPQLWVKTALGSKWGFVVAWLLWVQMFPGMVMVA
                     STLGPLLGNTFGNVELGNNHWFVLGCILVIYWIITILNLKFDMAKVGGNIGVWLGVYI
                     PVVIMFVLGVLAAFKVGLVSNGYLGDFSWSKAFPDLEHIDSLKYLAGITFIFVGIEMS
                     SVYMPRLKDATKNYTKGVFIALIGLVLLNVINAMLVANVVPDGKMELANITQPILIDC
                     QILGLPEVIGNIFSFMVFIGVLLQLSAWVTGPSKTIIQVAREGFLPPKFGFHKENKYG
                     VSRNVVLTQSIVISLFALLYGVMDDVSAVFLTLTNATTVIYCIVYILIAVSLLKMRKK
                     HPEFERPYRIGKNGNGLAWVVSCMLIFSIIVVVFATLGTATLSDALLVAAITVVMFVI
                     PLIINHFKKDSWGIEVEKSLEEK"
     gene            complement(3216..5093)
                     /gene="tdc"
                     /locus_tag="IWO89_001455"
     CDS             complement(3216..5093)
                     /gene="tdc"
                     /locus_tag="IWO89_001455"
                     /EC_number="4.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine decarboxylase"
                     /protein_id="HAQ0456206.1"
                     /translation="MSESLSKDLNLNALFIGDKAENGQIYKALLNELVDEHLGWRQNY
                     MPQDMPIITPEEKSSASFEHTVNKTKDVLSEISARMRTHSVPWHNAGRYWGHMNSETL
                     MPSLLAYNFAMLWNGNNVAYESSPATSQMEEEVGMEFAKLMSYKDGWGHIVADGSLAN
                     LEGLWYARNIKSLPLAMKEVTPELVAGKSDWELMNLSTEEIMNLLDSVPEKIDEIKAH
                     SARSGKHLEKLGKWLVPQTKHYSWLKAADIIGIGLDQVIPVPVDHNYRMDINELEKIV
                     RGLAAEKTPILGVVGVVGSTEEGAIDGIDKIVALRRVLEKDGIYFYLHVDAAYGGYGR
                     AIFLDEDNNFIPFEDLKDVHYKYNVFTENKDYILEEVHSAYKAIEEAESVTIDPHKMG
                     YVPYSAGGIVIKDIRMRDVISYFATYVFEKGADIPALLGAYILEGSKAGATAASVWAA
                     HHVLPLNVTGYGKLMGASIEGAHRFYNFLNDLSFKVGDKEIEVHPLTYPDFNMVDYVF
                     KEKGNDDLVAMNKLNHDVYDYSSYVKGSIYGNEFLTSHTDFAIPDYGNSPLQFVNQLG
                     FSDEEWNRAGKVTVLRASVMTPYMNKEEHFEEYAEKIKAALQEKLEKIYADQLLASEA
                     K"
     gene            complement(5384..6640)
                     /locus_tag="IWO89_001456"
     CDS             complement(5384..6640)
                     /locus_tag="IWO89_001456"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HAQ0456207.1"
                     /translation="MNIIDELTWRDAINQQTNEERLRELVEEKSISLYCGVDPTGDSM
                     HIGHLIPFMMMKRFQLAGHHPYILIGGGTGTIGDPSGRKTERVLQTMEQVQHNVDALS
                     NQMRKLFGKDANITFVNNYDWLSKISLLEFLRDYGKNFNINTMLAKDIVASRLEVGIS
                     FTEFTYQILQSIDFLHLHKTYDVQLQIGGADQWGNITAGLDLIRKLEGPEVEAFGLTI
                     PLMLKADGTKFGKTAGGAVWLDPKKTSPFEFYQFWLNQDDRDVVKYLKFFTFLSQEEI
                     EDLAKKVETEPEKREAQRRLAEEVTRFVHSEEDLKEAQKITQALFSGNIKELNAEEIA
                     QGFGKMPNVEISSTPENIVELLVSTKIEPSKRQAREDVSNGAISINGDRVTDLNFVIN
                     PSDEFDGKFVVIRKGKKNYFLAKVID"
     gene            7220..8521
                     /locus_tag="IWO89_001457"
     CDS             7220..8521
                     /locus_tag="IWO89_001457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HAQ0456208.1"
                     /translation="MEKKQKIILGATSALTIVILILSLFFVIRSSTKKEPIIPEKKIT
                     EKAKEPTDDKQQLVTQAKNLAQSYDYDQAIALLEKDSKNGSTETQQLLQTLKKEKEQL
                     IDWSDPTQISHVFFHSLIVDPQKAFHSQQAQGYKDYMVTVEEFNRSIEQLYQNDFVLV
                     NLNDLIQKDEQGNLSFNGLALPEGKKPLVLSQDDVSYYEYMDNSGFPSRLVVSNNNEI
                     KNVYKEKDKEEIGDFDMVPLIDAFVKKHPDFSYRGAKGTLALTGYNGVLGYRTSKSQY
                     GDNEKTKKEIKEAKKVAEQLKKDGWSFASHTWGHLNMTQASLEDVKKDNELWQQEVAP
                     ILGKTNILIYPFGADISDWQPYSAENQKFAYLKSQGFDIFCNVDASTPAWGQLGTDFY
                     RNARINIDGIRFQSDLDGKNPILDPFINVHDVYDKEARESA"
     gene            complement(8590..9189)
                     /locus_tag="IWO89_001458"
     CDS             complement(8590..9189)
                     /locus_tag="IWO89_001458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HAQ0456209.1"
                     /translation="MNGFEKRAQEKKKEILQAAFDLMNTDIGTAGVTMDKVAENAQVS
                     KATIFKYFGSKEGLIYEVFFAFMNDLGQEAKALIAENLPFEETLIAMSENKIRYLNRV
                     NKQFYIDLMTYYTKKEKKEFSDAMDAYTQESFSIMLDLFHRGRKEGKVDLKYSDEFLI
                     LYFQAMVEGISKPEIYAKLLPYTAEWTEVLIKGIAPKKK"
     gene            9443..10324
                     /locus_tag="IWO89_001459"
     CDS             9443..10324
                     /locus_tag="IWO89_001459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ0456210.1"
                     /translation="MAEIVKVTHLKKSFGRFEALKDVSFTVHSGEVVGFIGPNGSGKS
                     TTIRTLLGIIKKDGGTATIFGQDVWEDSLSIHERLSYVPGDISLWGNLTGGAIIDLFM
                     KLHGKGSAQKKDELIQRFSLDPKKKAKNYSKGNRQKVGLIAALAVDSDLYLFDEPTSG
                     LDPLMETIFQEEVEKLKEQGKSILLSSHILSEVERLADKVVIIREGTVVEEGTLDELR
                     HLTRSTITIETEAENSHLAQLNGVHDFVQKGHLAQFAADNEAFDVILAEASKLGVKKF
                     ESIPPTLEDLFMRHYEG"
     gene            10337..11947
                     /locus_tag="IWO89_001460"
     CDS             10337..11947
                     /locus_tag="IWO89_001460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetronasin resistance protein"
                     /protein_id="HAQ0456211.1"
                     /translation="MTDKFSRWSTLFSQNLSRDWKKILIWLVGVTAFSGGFVPAFEEL
                     TSGQGLAGLYETLQNPAMIAMVGPTPASNAAEYTLGAMYANEMLLFCSLFAAVISVLH
                     VVSQTRKKEELGLIELIRSFRVGRQANSLAVIAESILINLLLAISISCTMIFFQADSI
                     TAEGAWLFGLSVGSMGILGSAVALFAAQLMPSSASSNGLSLAFLGGLYLLRAITDVAD
                     SSLSMWNPLGWSYLTYPFTTNNWIPLLYLFLFSLILIISSFVLEEHRDLNTGYIPERE
                     GKPYAKRSLLSLRGLLFYLNKGMTISWLITFGLMGAAYGSIYGNMQQFLESNDLMKQM
                     FIQSGVSIEKNFTSTIMVVLICLAAILPIASINRLFTEETHGRMNQLFATKISRNQLY
                     WTNMLLAILFAILAILISSGALGLSALASMKDAKDMDLLMFLKAGFNFLPAVLVFIGI
                     SGFLFGWLPRMGKLAYIYLAYSFALNYFGGILDLPEWFSKTAVPSWVPRMPNDSFDLS
                     VFLTLSCISLALFVLGSIGYRRRDLLEG"
     gene            complement(12024..12683)
                     /locus_tag="IWO89_001461"
     CDS             complement(12024..12683)
                     /locus_tag="IWO89_001461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HAQ0456212.1"
                     /translation="MSHDHLCVSFVPLFNHLVQQDQEKVHALVKHHYVEKGEQILAPN
                     GEPQLVIVASGSMKVYQLSASGKEQLLRVIEPGGYEGESQLLGVPNESLFGEALEKTE
                     ICILRQKDFSELLLHYPALSLKLLAINVEKLTKVQQQTTFLMMEKVEERVAVYLLDLA
                     KATGKDHFFLPMKMKELAAFIGTTPETLSRKFKLLEENGYVKRKNREVWIIDKEQLED
                     L"
     gene            complement(12966..13631)
                     /locus_tag="IWO89_001462"
     CDS             complement(12966..13631)
                     /locus_tag="IWO89_001462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0456213.1"
                     /translation="MELGKSDYQLFDRPIYAFKQLKESHPTDKIEQIKKEYKEHWQKW
                     KEIQLQTAALLPDMYGMSKPKIESWTNGWNLRSHFWSAYRSESRQDENACLAVLLNQK
                     QYQIYLMYQHYKSEERYGSIAAYNQLLAILKEWSKTVDIKDYYIWPQPEHELDDHLAL
                     SDYLSDTKKQEELKKAMRDRTFQMGKLFFYPQEIEHVEQITAETLQELAPLYQKLGAE
                     KFQ"
     gene            complement(13778..14383)
                     /gene="dhaL"
                     /locus_tag="IWO89_001463"
     CDS             complement(13778..14383)
                     /gene="dhaL"
                     /locus_tag="IWO89_001463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit L"
                     /protein_id="HAQ0456214.1"
                     /translation="MKLTVQEIQEWLTQFAEAINQNKQYLSDLDTPIGDGDHGNNMGR
                     GVSAYEEAFQADHPETISDTFKVFSMAMISKVGGASGPLYGSAFMNMMKATKEVDSID
                     SQEKLGEVIEQGTAGIQSRGKAEADDKTMLDVWLPVTEALKAGNLTKEIIDQAKEHTK
                     VLVAKKGRASYLGERAIGHIDPGAASSAILFSTLLDVINQP"
     gene            complement(14411..15394)
                     /gene="dhaK"
                     /locus_tag="IWO89_001464"
     CDS             complement(14411..15394)
                     /gene="dhaK"
                     /locus_tag="IWO89_001464"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit DhaK"
                     /protein_id="HAQ0456215.1"
                     /translation="MKKIMNDPSNIVEEMLEGLVKSYPELVHRVESSRVVAKNQKAEQ
                     VGLVSGGGSGHEPSHAGFVGEGMLSAAVLGDVFTSPTPDQIQTAIKEADSGKGVLLIV
                     KNYTGDALNFDMAKELAAMDDIEVESVIVDDDIAVENSTYTAGKRGVAGTVLVHKIVG
                     DAARNGASLAELKELGEKVVQATKTIGLALRAATVPEVGKPGFELGEDEIEYGVGIHG
                     EPGYRREKMQPSKVLAKELVTKILDDYSNLPKEAGVLVNGMGGTPLMEQFVFMNDVLA
                     LLEERGVNVVFRKVGNFMTSLDMQGLSLTLIDLTETQWKDSLESNVQTISW"
     gene            complement(15396..15791)
                     /gene="dhaM"
                     /locus_tag="IWO89_001465"
     CDS             complement(15396..15791)
                     /gene="dhaM"
                     /locus_tag="IWO89_001465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS-dependent dihydroxyacetone kinase
                     phosphotransferase subunit DhaM"
                     /protein_id="HAQ0456216.1"
                     /translation="MKKSILLISHSQKVTDGIKEMIEQMQKSEEVQIFSLGGINGEIG
                     SDPMKIVEAVNESSDAASYFVFADIGSAVLNSELAKDMLEEEQQQRYHLVDAPLVEGA
                     FAAAITAGITDDTQQILTEAQNAGKKGWE"
     gene            15945..16265
                     /locus_tag="IWO89_001466"
     CDS             15945..16265
                     /locus_tag="IWO89_001466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal-binding domain-containing protein"
                     /protein_id="HAQ0456217.1"
                     /translation="MITTTTNSVEGKAVVAYKGIVFGEVITGIHAFKDLEAGLRNIFG
                     GRSKSYENELMGAREEALAEMAERAKALGADAIIGVKMDYEVLGSDNGMLMVTCSGTA
                     VSTR"
     gene            complement(16377..17246)
                     /locus_tag="IWO89_001467"
     CDS             complement(16377..17246)
                     /locus_tag="IWO89_001467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015370988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HAQ0456218.1"
                     /translation="MGKVMKKRMKPLIWPIAVSSIFLLGQTAFAETSEPAQTEPSTTI
                     SSSGAENGASETEMTSNVPTDNSGETISSSEDTSINSSTSSDQIDHLQDLENKRKELE
                     EALEAAKNKLKEDQAAIDQDLADLKNKVGNDRAQLEKKLAEAKAKAEAVQTDLDGKIQ
                     QAKEFNQQLNQVAQTASGKLSQTQADITAASQAIHGKISEAQTVANDFEAKKADLAAS
                     FQRIKDILHSAGEKSVTSGSTTETVQQTTEPSKSTSQTLPKTNEKTASVSYSLAGAGV
                     LVAAAYGIIRKKK"
     gene            complement(17342..18616)
                     /locus_tag="IWO89_001468"
     CDS             complement(17342..18616)
                     /locus_tag="IWO89_001468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003745485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HAQ0456219.1"
                     /translation="MLRYLFVYYVFFLVSYPILGGFVWFIGVWCYVFLYKHKQKEWVD
                     VPLSVEPFITIMVPAHNEEIVIEDTIEYLMTKLNYHNYEVLVTDDGSTDQTPEILARL
                     MKKYANLRVVRIEKNKGKAHAFNIGLAFAKGKLILSNDADTVPEPDALIRYVNYFIRP
                     GARHIAAVTANMDVQNRTKLIAKSQTVEFSSIVGIIKRTQSAVFGGLYAYSGANTMYR
                     KEALIDVGGFRQDRATEDISIAWDHQLNDWLSVFAPEIIFFMEVPVTLKMLYRQRKRW
                     AKGGTEVWLTNFKKVMLHPFKHIGRTIIFIDQTLSIVWSIFFCISVVLFAGLIGHYVY
                     QGNYEQIYITFTFSFVFICFEMVAGFFQLLASLIVDDRRRKLKYLLFAPLYMLLFWIV
                     NAITIVTTFIPAVKTILGYGSGTWKSPERTKK"
     gene            complement(18642..19151)
                     /locus_tag="IWO89_001469"
     CDS             complement(18642..19151)
                     /locus_tag="IWO89_001469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0456220.1"
                     /translation="MKKVTREEVASILVKDKYFSKGNYWLKIWQTLVALLGWMIVVLP
                     FIWVFFPLTRPERAKDFSLYAFLSEKKMLYFLIGFFVLIFFSFLITSFVLTRWNNRNL
                     YKKVNKSFEYEDEKLKKRQELLEEVYTERFGTKEERETVRFYSVSEEKNLDTTFIADL
                     YKENGVELK"
     gene            complement(19326..21461)
                     /locus_tag="IWO89_001470"
     CDS             complement(19326..21461)
                     /locus_tag="IWO89_001470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HAQ0456221.1"
                     /translation="MVNTQKQLEEAYLKNVYQKLIDKKNELRESMDTAKIEGNQSLKE
                     MAGELRLNFDSYLDNLDTFSMIEMKNREIDQMNIKQKNAENQLAKIERLLKSPYFGKV
                     VVDFLDNEPKESFYIGTANFTDDADENLVYDWRSPIAELFYNNMIGPSSYTVRQNKID
                     TVIKERRQFLLERDRLVKFFDTSVAIQDDVLLEVLGQDETNEMKAITATIQSEQNTII
                     RDVKSQNILVNGVAGSGKTSAIMQRIAYLLYLYRDTMTSDDLLILSPNHRFIDYISNV
                     LPSLGERNPLNLTIIQLVSQLSAEEIEGEEAYFKRITGENVSEQTERLRSKKFIDNLK
                     QSDPLFLDHPNFIRGLTKNGKTVLSKKTIEKIYEKVPAHPKLIDRLQATKKALMSEWK
                     NHLLKQAKSPAVQNQVLSLTEDRQLELFGKLISDDSEQSIVAYARKLLQKKYRKITRQ
                     IEEMAWVDEHQLFERIYEKRYGSAYAWQPTRTVDEAVIILAIRHLLVEKVNVPAFRYL
                     LIDEVQDYTLAQLGLLIELFPKTHFTLVGDENQAIFNSSTTFADIMRCFDDYHLPIHR
                     YDLRNSYRSSGAITELFKTYAVDQEKIDIVSIRPQGKEPEYRSFRSSEELLKILAEIL
                     VSLKGEKAAVITQTEEETQTLQESIKKNSYGLENCQIIPLSLAKGLEFDHVILYPFEN
                     DGDEQRRRRQMYTAISRGMKSIVVLERAT"
     gene            complement(21544..23199)
                     /locus_tag="IWO89_001471"
     CDS             complement(21544..23199)
                     /locus_tag="IWO89_001471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HAQ0456222.1"
                     /translation="MKIVIVGGVAGGMSAATRLRRLMEDAEIVVFEKGPYVSFANCGL
                     PYYLSGEISERENLLVQTPESLSARFCLDVRPNHEVTAIFPENKTVEVVHEGQKHIEQ
                     YDALVLSPGAKPVVPSIPGITEADNVFSIRNVPDIDKVMHALEKQPKRAVIVGAGFIG
                     LEMAENLKRRGLEVMVIEQAPHILPTLDEEMAAFIEKELSHQGVEVITSHAVAGFEDH
                     GKRLRLDDGRTIPADLVILSIGVRPDNQLAVTAGIELGIRGGILVDERYQTNIPDIYA
                     VGDAIVVKQQITGKDALISLASPANRQGRQVADTISGISRRNQGGIGTAIIRTFGMTA
                     ASTGLSERTAKENELSFEVIHVSGKDHASYYPEATDILLKLIFHPETGEIYGAQGVGA
                     KGVDKRIDILATAIKGHLTIFDLPELELTYAPPFGSAKDPVNMLGYAAMNIAEGLSET
                     VQWHELPTELAKGKILLDVRTAEELEKGKFKEAKHIPLNELRDRLNELDSQQEYIVSC
                     HSGLRSYLAERILKQSGYHVKNLDGAFSLYQTVRPEELIYPNK"
     gene            complement(23201..23515)
                     /locus_tag="IWO89_001472"
     CDS             complement(23201..23515)
                     /locus_tag="IWO89_001472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010730940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HAQ0456223.1"
                     /translation="MNLLFQSIKSITVPELKEKLLENPALLDVRTPAEYRGGHIKGAK
                     NVPLQSINRYDGDKEKTVYVICQSGMRSKQAAKELKKSGYDVVNVRGGMNQWFDRTVG
                     GK"
     gene            23685..23945
                     /locus_tag="IWO89_001473"
     CDS             23685..23945
                     /locus_tag="IWO89_001473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sensitive transcriptional regulator"
                     /protein_id="HAQ0456224.1"
                     /translation="MEIDNKNILNRLKRTEGQIRGIQKMIEDEKECMDVITQLSAVRS
                     SIDRVMGMIVADNLKNCFEKPDSNPEEQAAKLEQAIKMIIKK"
     gene            23959..24273
                     /locus_tag="IWO89_001474"
     CDS             23959..24273
                     /locus_tag="IWO89_001474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfur reduction protein DsrE"
                     /protein_id="HAQ0456225.1"
                     /translation="MKVVFHIDELEKWSETGKNVKNLIKASPETTIVVSVNGIAITGY
                     LDSANAEFLDLQGVVFHACANAMRANHISESSLPEQVIVVPAGVLDLVELQSQGYAYI
                     KP"
BASE COUNT         6949 a         5044 c         4397 g         8000 t
ORIGIN      
        1 aaaagcaatt ttcttgagtt tcttcttatt tcttggagtt aaacgcttct gtcccagcct
       61 cttttttcct tccttcaatt ttttacacgt cgacgattca ataaaaaacc gcctaatatt
      121 gtaaagacag gtgcaagaat cgagaaaaag acgaatggca agtaatgcaa cgcgctaact
      181 ttcatcgttc ccataatgaa tgtgccgctg actccccacg gtacgagaga attgacggtc
      241 gcacctgaat ccgctagtgt tcgtgtcaaa taggtttttg ataagccaac gtcatcaaaa
      301 gaacgcttga atgtttctcc tggaagaatg atggacagat actgttctcc aacgatcaga
      361 ttgattccga tacagctcaa ggcagtaaac ccgactaaac gagaaggtcg gtccatcttt
      421 tcttttaatt cttgaacgat ggtatcaacg atcagatatt tgatcagtaa cccacctaat
      481 gccaatgcaa gtaaaatcaa actagcagaa cctaacatac tcatgatccc gcctcgagaa
      541 agcagttcat ctagctttgg atcaggtgta tttgcgacat aaccattcat caagatgtct
      601 gctgcttttc cgaccgtcaa acttttctca tggataaaag aaagaacgag agcagctgtc
      661 gatcccgcta gtaatgtagg gatagccgga atttttttga ttgccccccc aaataaaata
      721 actagtggaa tcaatgtcca aggagagatc caaaagctcg ctttcatgtt ttggatcatt
      781 tcattgatac tagctagatc ggctgaatga ctgttatgtc ctaatatcgt ataaaaaatc
      841 aacgaaagga cgaatgctgg gaaatccgta taacgcatac cagcgatatg ttcgaataga
      901 ttaactcctc caatacccgc tgccaagttg gtcgtatctg ataaaggcga gcagttattc
      961 cctaaaaagg caccggaaac gactgcgcct gtagtcattg catcattgaa tccaagtaaa
     1021 tgaccgatcc ccaaaaaagc aattccaatc gtcgaaatcg ttgtaaatga actgccgaca
     1081 gtcactccga caattgcaca aacaagaaat gctgtcggta aaaagaaacg cacagaaaca
     1141 agtttaaatc cataaaccat aatcgtaggg atggttccag ataaaatcca tgaagcaact
     1201 aagacaccaa tcaaaagaaa aatcaggatt ggaatgatcc ctggagtgat tcctttgatg
     1261 atcccgtcat ggatctcatc ccatgtgaac tttttaaatc tgccataaaa caacaaacac
     1321 atgaaagctg caaggatcgg gatatgtggc gagagctgga atccgatgat cagtactcct
     1381 aggagtgcca acaacacgat caatacagcg atactttctt ttaaagaaat atttttattc
     1441 atcgtcgatc tcctatttca aatgaaaagg ccctgccatg aaaaaactat tcacttggca
     1501 ggacctccta ctaaatcagc gacaaaacgt cggctgaaag acttattttt tttatttttc
     1561 ttctaaactt ttttctacct caatccccca agaatctttc ttgaagtgat tgatgatcaa
     1621 tggaataacg aacatgacca ctgtgatagc tgctactaat aatgcatcac tcaatgtcgc
     1681 tgtacctaat gtagcaaata cgacaacgat gatagagaag atcagcatgc agctgactac
     1741 ccaagctaat ccgtttccgt ttttaccaat tcggtaagga cgttcgaatt ctggatgttt
     1801 cttacgcatt ttaagcaatg aaacagcaat caaaatgtac acgatacaat aaataacagt
     1861 tgtagcattt gtaagtgtca agaatacagc acttacgtca tccattacac cataaagcaa
     1921 agcaaataat gaaataacga ttgattgtgt tagaacgaca tttctagata cgccgtattt
     1981 gttttcttta tggaatccga attttggtgg aaggaatcct tcacgagcta cttggatgat
     2041 tgtttttgat ggtccagtaa cccaagctga taattgtaaa agcacaccaa taaataccat
     2101 gaagctgaag atatttccga tcacttctgg taagcctagg atttgacagt caatcaagat
     2161 aggctgcgtg atatttgcta attccatttt tccatctggt acgacatttg ctacaagcat
     2221 cgcattgatg acattcaata aaactaatcc gatcaaagcg ataaatacgc cttttgtata
     2281 attttttgtt gcgtctttca aacgaggcat gtaaacagaa ctcatttcga tcccaacaaa
     2341 aataaatgtg ataccagcta agtatttcaa tgaatcaata tgttccaagt cagggaaagc
     2401 ttttgaccat gaaaagtcac ctagatagcc gtttgagact agtccgactt tgaaggctgc
     2461 caaaacacct aaaacgaaca tgatgactac tgggatatat acccctagcc atacaccgat
     2521 gttcccgccg acttttgcca tatcgaattt caagttcaag atcgtgatga tccagtagat
     2581 aactaaaata caacctaaaa cgaaccagtg attattgcca agttctacat taccaaatgt
     2641 gtttcctaac aatggtccta aagtagaagc gaccattacc atacctggga acatttgcac
     2701 ccataatagc catgcaacga caaatcccca tttgctgccc agtgctgttt ttacccataa
     2761 ctgaggaccg ccttcttgag gaagcatcgt ggaaagctca ccggaaacca ttgatatcgg
     2821 accggcaaaa aacaaaactg caaaaattga ataaaaaatc aatgtccaac cgactgcggc
     2881 taacgtagga atactccgaa ccgttgcaca cagtgccatt gtcagaccga taaaggtccc
     2941 aagacccatt ttacttactt cagatttact caccgttgac actctccttg attttttgga
     3001 taaaataacg gtgtctttga agtagtttca gcaataaacg aaacaattca gcgaatattt
     3061 gaggggattc tttgagtcta ttgcttgaag ggtttcttca aagacaacct caaaacaaag
     3121 ggatcgaaga gcttaggtct ccccttgctc ttcagatcca aacatgcgtc agaaacagtt
     3181 agtattcaag ctgttttcta acatgattac cttgcttatt ttgcttcgct tgccaataat
     3241 tggtctgcgt agattttttc caatttttct tgaagagctg ctttgatttt ttcagcatat
     3301 tcttcaaagt gttcttcttt gttcatgtat ggtgtcatta cgctggcacg caatacagtt
     3361 acttttccgg cacggttcca ttcttcatct gagaatccta gttggttcac aaattgtaat
     3421 gggctgttgc catagtctgg gatagcaaag tcagtatgtg aagttaagaa ctcattaccg
     3481 taaatgcttc ctttcacgta tgaagaataa tcatatacgt catggttcaa tttattcatt
     3541 gcaactaagt catcgttgcc tttttctttg aatacatagt ctaccatatt gaaatctgga
     3601 taagttaatg gatgaacttc gatttctttg tctcctactt taaatgacaa gtcattcaag
     3661 aagttgtaga aacgatgtgc accttcgatt gatgcaccca ttaatttacc atatcctgtg
     3721 acattcaaag gtaagacatg gtgtgctgcc caaacgctgg ctgctgttgc ccctgctttt
     3781 gaaccttcca agatataagc cccaagtaat gctgggatat ctgcgccttt ttcaaataca
     3841 tatgtcgcaa agtaagagat aacgtctctc atgcggatat ctttgatgac gataccaccg
     3901 gcagaatatg gaacatagcc cattttatgt gggtcaatcg ttacagattc tgcttcttcg
     3961 attgctttat atgcactgtg tacttcttct aagatataat ctttgttttc tgtaaagaca
     4021 ttgtatttgt aatgaacatc ttttaaatct tcaaatggaa tgaagttgtt gtcttcgtct
     4081 aagaaaatcg cacggccgta tccgccataa gcagcatcta cgtgtaagta gaagtaaata
     4141 ccgtcttttt ctaatacgcg acgcaatgca acgattttat cgataccgtc gattgctcct
     4201 tcttctgttg atcctacaac accgactaca cctaggattg gtgttttttc agctgctaga
     4261 ccacgaacga ttttttcaag ttcgttgatg tccatgcgat agttgtggtc aactggcact
     4321 ggaatcactt ggtctaaacc gataccgatg atgtcggcag cttttagcca tgaatagtgt
     4381 ttagtttgtg gaaccaacca ttttcctaat ttttctaaat gttttccgct acgtgctgag
     4441 tgagctttga tttcgtcgat tttttctggg acgctgtcta acagattcat gatttcttct
     4501 gttgacaagt tcattaattc ccaatcgctt tttccagcaa ctagttcagg tgtcacttct
     4561 ttcattgcaa gcggcaatga tttgatgttg cgggcatacc aaagtccttc taagtttgct
     4621 aaagaaccat cagcaacgat gtggccccag ccatctttat agctcattaa ttttgcaaat
     4681 tccattccta cttcttcttc catttggctt gttgcaggag aagattcata ggcaacgtta
     4741 ttaccattcc atagcatagc aaaattgtac gctagtaaag atggcatcaa tgtttcagag
     4801 ttcatatgac cccagtaacg tccggcatta tgccaaggaa cggaatgggt acgcatccgc
     4861 gctgaaattt ctgatagaac atcctttgtt ttattcacag tgtgttcaaa actagcactg
     4921 cttttttctt ctggcgtaat aataggcatg tcttgaggca tgtagttttg acgccagcct
     4981 aaatgttcat ccaccaattc attcaacaaa gctttataga tttggccgtt ctcagctttg
     5041 tccccaatga atagggcatt taggtttaga tctttcgaca atgattcact catagtgaaa
     5101 ccctcctaaa aaaatattta aattttgtcg agccctacat tacacctgtt cttctattct
     5161 gtaaactaag ttttggcctg atttttaatc atattttaat atgtatcaat tatccgaatt
     5221 gttcacactt ttgaaataat taaaaaaaac gagggaattt acaaatgtaa actccctcgc
     5281 aaagattgta ttaacgacaa ccagcgttct attttaggat gggtttcaaa attggaatgt
     5341 tgtaatagta tttccctttt tcaaggtaaa aacttactaa attctaatca attactttgg
     5401 ccaaaaagta atttttctta ccttttcgaa taaccacaaa cttaccatcg aattcatcgg
     5461 atggatttat gacaaaattt aaatcggtaa cgcggtcacc gttaatactt atagctccgt
     5521 ttgaaacatc ttcacgtgct tgacgtttag atggttcaat ttttgtggaa acaagcagtt
     5581 ccacgatatt ttcaggtgtg cttgaaattt caacgttcgg cattttaccg aatccttgcg
     5641 cgatttcttc cgcatttagt tctttaatat ttccagagaa caatgcttgc gttatttttt
     5701 gcgcttcttt caagtcttct tcactatgca caaatcttgt aacttcttct gccaaacggc
     5761 gttgtgcttc acgtttctca ggttctgtct ctactttttt cgctaagtct tcgatttctt
     5821 cttgagacaa gaaagtaaag aatttcaagt atttcactac atcgcgatca tcttgattca
     5881 accagaattg gtagaactca aatggtgaag ttttcttcgg atcaagccaa acggcaccgc
     5941 ctgcagtttt tccgaatttc gttccgtcag ccttcagcat caatgggatc gttaaaccga
     6001 acgcttctac ttctggtcct tctaattttc ggattaggtc taatccagct gtgatatttc
     6061 cccattggtc tgctccaccg atttgcaatt gcacatcgta tgttttatgc agatgcaaaa
     6121 agtcgataga ttgcagaatt tggtaagtaa actctgtaaa tgagattcct acttccaaac
     6181 ggctggcaac gatatctttt gctaacatcg tgttgatgtt gaagttttta ccgtagtctc
     6241 tcaaaaattc aagtaaagag atttttgaca accagtcgta gttgttcaca aatgtgatat
     6301 ttgcatcttt accaaacaat tttctcattt gattggaaag tgcgtccacg ttatgttgca
     6361 cttgttccat cgtttgcaac acacgttctg ttttgcggcc gcttgggtcg ccgatcgttc
     6421 ctgttccgcc gccaatcaga atatatggat gatggcctgc caattggaat cttttcatca
     6481 tcataaatgg aatcaaatgt ccgatatgca tcgaatcacc agttggatca acgccgcaat
     6541 aaagtgaaat gctcttttct tcaacgagct cgcgaagtct ttcttcgttt gtttgctgat
     6601 taatggcgtc gcgccaagtt aattcatcaa taatattcat tttactgacc tccgtgtagt
     6661 ttttttatga aagcaggaac ctgctagcat gtgtaattaa ttatttcttc aggataggat
     6721 aggaaataaa aaaagtccct tggcatataa gccaagggac gaaatctcgc ggtaccaccc
     6781 aatttgtatg tctagatatc caacatacct ctcgaacatt taacgctgtc gcgtggcaat
     6841 cactgccaaa gactcctgaa atgtaattcg ctttaaagca tgtactggtt tgcagcgacc
     6901 accagctttc tgaaacattg gctttaacaa ctacttcgtt tcgatcttcg tcttgttatt
     6961 attaatatgt gtctatcata gagcgcaact ttttctttgt caacagaaaa aggatatttt
     7021 ttttaaattc ctcatttttt cttttttgaa tccgctctaa ttccgaacat tttattttca
     7081 attattgtta aaagaaataa ctttttctgt attttttctg caatttatcg agaagtcact
     7141 ttactttgcc tcttattctt tcttcgttac aataaggaat gggacaagta aaaaaatgaa
     7201 aaagcaaggg acgaggtata tggaaaaaaa gcaaaaaatt attttaggag ccacttctgc
     7261 tttaactatc gttattttaa ttctaagttt gttttttgtc atccgatcat ctacaaaaaa
     7321 agaaccaatc attcctgaaa aaaaaataac agaaaaagca aaagaaccga cagatgataa
     7381 gcaacaacta gttactcaag caaaaaatct ggctcaaagc tacgattatg accaagcgat
     7441 tgcgttattg gaaaaagata gtaagaacgg atctactgaa acacaacagc ttcttcaaac
     7501 actaaaaaag gagaaagaac aactgatcga ctggtctgat cctactcaga tctctcatgt
     7561 ctttttccac agcttgatcg tggacccgca aaaagccttc cactctcaac aggcacaagg
     7621 atacaaagat tatatggtga ctgttgaaga attcaaccgt tcgatcgaac aactttatca
     7681 aaatgatttt gttttagtta atttaaatga tttgatccaa aaagacgaac aaggaaatct
     7741 ttcctttaac gggcttgcgc tacctgaagg aaaaaaacca ttggttcttt cacaagatga
     7801 tgtcagctat tatgaatata tggataattc cggttttcct agccgattgg tcgtatcaaa
     7861 taacaatgaa atcaaaaatg tctataaaga gaaagataaa gaagaaatcg gtgactttga
     7921 catggttcct ctgatcgatg cctttgtcaa gaaacatcct gatttttctt atcgaggcgc
     7981 aaaaggtacc ttggccctga caggttacaa cggagtttta ggttatcgaa catcaaaatc
     8041 tcaatatgga gacaatgaaa aaacaaaaaa agagattaaa gaagctaaaa aagtggcgga
     8101 acagttgaag aaagacggct ggagctttgc ttcccataca tggggtcact tgaatatgac
     8161 tcaagcttca ctagaagatg taaaaaaaga caatgaattg tggcagcaag aagttgcacc
     8221 tatcctagga aaaacaaata ttctgattta tccatttggt gcagacatca gcgactggca
     8281 gccttattca gcagaaaatc aaaaatttgc ttacttgaag agtcaaggtt tcgatatttt
     8341 ctgtaatgtc gatgcttcta caccagcttg gggacaatta ggtacggatt tctatcgaaa
     8401 tgctcggatc aatatcgatg gcattcgttt tcaatctgat ttagacggaa aaaatcctat
     8461 tcttgatcca ttcatcaatg tccatgatgt ctatgacaaa gaagcacgcg aatctgcttg
     8521 attttacgtc atttatgaac caaaaaagcc gaactgacat tttatcgtca gtttcggctt
     8581 ttttatgctt tatttcttct tcggtgcgat tcccttgatc aggacttctg tccattctgc
     8641 tgtatatgga agcagttttg cgtatatctc cggcttagaa attccttcta ccattgcttg
     8701 aaaatagagg atcaagaact catccgaata tttcaaatcc acttttcctt ctttccgccc
     8761 tcgatggaaa agatcaagca tgatactgaa actttcttgc gtatacgcat ccatcgcgtc
     8821 agaaaattct tttttttctt tttttgtata gtatgtcatc aaatcgatat aaaattgttt
     8881 gttcactcga ttgagataac ggattttgtt ttcactcatg gcaatcagtg tttcttcaaa
     8941 tggaagattt tccgcaatca acgctttcgc ttcttgccct aaatcattca taaatgcaaa
     9001 aaatacttca taaatcagcc cttccttgct gccaaaatat ttaaaaatcg tcgctttact
     9061 tacctgagcg ttctcagcta ccttatccat tgtcactcca gcggttccta tgtctgtgtt
     9121 catcaagtca aaagctgctt gtaaaatctc tttctttttc tcttgtgctc tcttttcaaa
     9181 accgttcatt tgtagagcct cccgtaattt taaactttta taatatatat agttcaaatt
     9241 tattctgagg tttattataa ctatctattg agaaagtatc aagctagcaa ttccttgttt
     9301 atgaattcat tgacagaaat ttataaaaag aatattatgt actaaaataa taaaaaaagt
     9361 tttataaaaa ggtataaaac ctttttatta aactatttaa tcttgtttag tatatatttt
     9421 tagaaaaagg agtggggttt ttatggcaga aatcgtcaaa gtcacacatt tgaaaaagag
     9481 ctttggtcgt ttcgaagctt taaaagatgt ctctttcacc gtacattcag gtgaagtcgt
     9541 tggatttatc ggaccaaatg gttctggaaa atccacgacg attcggacac ttttagggat
     9601 tataaaaaaa gacggcggaa cggcaacgat ttttgggcaa gatgtatggg aagatagttt
     9661 atctatccat gaacgtcttt cttatgttcc aggcgatatc tctttgtggg gaaatttgac
     9721 tggaggagcg atcatcgatc tcttcatgaa gcttcatgga aaagggagtg ctcaaaaaaa
     9781 agatgaactg attcaacgtt tctctcttga tcctaagaaa aaagcaaaaa actattcaaa
     9841 aggaaatcgc caaaaagtgg gcttgattgc tgctttggct gttgattcgg atctctattt
     9901 atttgatgag cctacttctg gtttagatcc tttgatggaa acgattttcc aagaagaagt
     9961 tgaaaaattg aaagaacagg gcaaatccat cctactttct tctcatattc tcagtgaagt
    10021 cgagcgacta gcggataaag tcgtgatcat acgcgaagga acagtcgtag aagaaggaac
    10081 actcgatgaa ttgcgtcatt tgactcgttc gaccatcact atagaaacag aagcagagaa
    10141 tagtcactta gcacaattga acggtgtcca tgactttgtg caaaaaggtc acctcgctca
    10201 atttgctgct gataacgaag ctttcgatgt gattttagct gaagcgtcca aattaggtgt
    10261 gaaaaaattt gaatctattc caccgactct tgaggattta ttcatgagac attatgaagg
    10321 atgaggagga agcgaaatga ctgataaatt ttcacgctgg tccacccttt tttctcaaaa
    10381 ccttagccgt gactggaaaa agatcctgat atggctagtc ggtgtcactg ctttttctgg
    10441 tggttttgtc cctgcgtttg aagaattgac ttccggacaa ggattagctg gattatacga
    10501 aaccttgcaa aacccggcaa tgattgcaat ggtcggacca actccagctt cgaatgctgc
    10561 tgagtatact ttaggagcga tgtatgcaaa tgaaatgttg cttttttgca gtttgtttgc
    10621 tgctgtgatc tctgttttac acgtggtgag ccaaacgaga aaaaaagaag aacttggtct
    10681 gattgaactt attcgttcct ttcgagtcgg acgtcaagca aactctttag ctgtcatcgc
    10741 tgaaagtatt ctgatcaatt tgcttttagc aatatcgatc agctgtacga tgattttttt
    10801 ccaagcagat tcgatcactg cagaaggtgc ttggcttttt gggctatctg taggtagtat
    10861 ggggattttg gggagtgccg ttgcactgtt tgctgcacaa ctgatgccaa gttcagccag
    10921 ttcgaatggt ctgtctttag cgtttttagg tggactttac cttctacgag caataacaga
    10981 tgtagctgat tcctcccttt cgatgtggaa tcctttaggc tggtcttatt tgacctatcc
    11041 atttacaact aacaactgga ttcctttgct ttatttgttt ttgttcagtc taatcttgat
    11101 aatcagttca tttgttttag aggaacatcg agatttgaac accggctata tccccgaacg
    11161 tgaaggaaaa ccgtatgcta aacgttctct tttatccttg cgtggtttat tattctatct
    11221 aaataaaggg atgacaatca gttggcttat cacttttggt ttgatggggg cggcatacgg
    11281 ctctatctac gggaatatgc aacagttttt agaaagtaac gacttaatga aacagatgtt
    11341 tatccaatct ggcgtctcaa ttgaaaaaaa ctttacaagc acgatcatgg tcgtcttgat
    11401 ttgtctagct gctattttac caattgctag catcaatcgt ttatttacag aagaaaccca
    11461 cggacgtatg aatcagctgt tcgcaacaaa aatttctagg aaccaacttt attggaccaa
    11521 tatgttacta gctattctat ttgctatatt ggcaatcctg attagttctg gtgcactagg
    11581 gctttctgct ttggcttcga tgaaggatgc gaaagacatg gatctattga tgtttcttaa
    11641 agccgggttc aatttcctgc ctgccgtttt agtgtttatc ggaattagtg gctttctatt
    11701 cggatggctt cctcgaatgg gtaaacttgc ttatatctac ttagcttact cctttgcact
    11761 caattacttt ggcggaattt tggatttacc agaatggttt tccaaaacag ctgtccctag
    11821 ctgggttcca cggatgccaa acgattcttt tgatctttcc gtgttcctta cgttatcatg
    11881 tatcagcctt gcactgttcg tccttggttc tattggttat aggcgaagag acttactaga
    11941 aggataataa aaaagcatgt acaatttcat gctagcctcc aaatctgggt caaaaatcca
    12001 acatttggag gtcatttttt tatttacaag tcttctaact gctctttatc aatgatccag
    12061 acttctcgat tctttctttt tacataacca ttctcctcta ataatttaaa ttttctagat
    12121 aaagtttcgg gggtcgtacc gataaatgca gcaagttctt tcattttcat tggcaggaaa
    12181 aagtgatcct tccctgtcgc tttagctaag tcaagcagat agacagcgac tcgttcttct
    12241 actttttcca tcatcaaaaa agttgtctgc tgctgtactt ttgttaattt ctcaacgttg
    12301 attgctaaaa gtttcagact aagggcggga taatgcagta agagttccga gaaatctttt
    12361 tgtctcaata tgcaaatttc cgttttttct agtgcttcgc caaataaaga ctcatttgga
    12421 acgcctagca actggctctc tccctcatat ccgcccggct ctattactcg taaaagctgt
    12481 tcctttccgc tagcagataa ttgatacact ttcatactgc cggaggccac gatcactaat
    12541 tgaggttctc cattcggcgc aagaatctgt tctccttttt ccacatagtg atgtttgaca
    12601 agtgcgtgta ctttttcttg atcttgctgt actaagtgat tgaacaaagg aacaaatgaa
    12661 acacataaat gatcatgact cataatctct cctccttcta aacagctatt tctaataaag
    12721 aaacataact tcccttattc caactgataa ttttctttta tcatagcata aacgtcaagc
    12781 gaaccagata ccagcaaata gagtggattt ctttttgtcc cttgctttat tttccttctt
    12841 ttgagacctt aagtaattga ttcctatcaa ttttttaaat atttactttt tgaataatca
    12901 aaaacaaacc ttcactcgca agccgagagt aaggtttgtt tttcgcattg cttatttcgt
    12961 gttgtctatt gaaatttttc cgcacctagc ttttgatata gtggtgctag ttcttgtaag
    13021 gtttcagcgg ttatttgctc aacgtgttcg atttcttgcg gatagaagaa tagtttaccc
    13081 atttgaaagg ttcggtctct catcgctttt ttcaactctt cttgtttctt tgtatcggac
    13141 aaatagtcag acaaagctaa gtggtcgtca agttcatgtt ctggttgcgg ccatatgtaa
    13201 taatccttga tatcgactgt tttgctccac tctttcagta tcgcaagcaa ctggttatac
    13261 gctgcaatgc taccatatcg ttcttcgctt ttataatgtt ggtacatcaa atagatttgg
    13321 tattgttttt gattcaacaa aactgctaga caggcattct cgtcttgacg agattcgctt
    13381 cggtacgcgc tccaaaaatg actgcgtaaa ttccagccat tcgtccagct ttcgatttta
    13441 ggtttgctca tgccgtacat atcaggcaaa agtgcagctg tctggagctg aatctctttc
    13501 catttttgcc agtgttcttt atattctttc ttgatttgtt caatcttatc tgttggatgg
    13561 ctctctttga gctgtttgaa tgcgtagatt ggtcgatcaa acaactgata atctgatttt
    13621 ccaagttcca ttgtttcgcc tctttccgtt atcttttgta ttctacctga attttgcgat
    13681 atggacaaat agaggttgtg aaaaagctgt tctgcatcaa gtcataagaa cagccaaaca
    13741 aaaaactgcg atgagcatca ccgcagtttt attgaactta aggttgattg atcacatcta
    13801 aaagcgttga aaataaaatg gcactagaag cagctcctgg gtcgatatgt ccgattgcac
    13861 gctctcctag ataggaagct cgtccttttt tagctaccaa gactttggtg tgttcttttg
    13921 cctggtcaat gatttctttt gttaaattcc ctgccttcaa tgcctctgtg actggcagcc
    13981 aaacatctaa catcgtttta tcgtcagctt ctgcctttcc tcggctttga atacccgctg
    14041 ttccctgctc gatcacttcg cctaattttt cttgagaatc aatagaatcg acctcttttg
    14101 tcgctttcat catgttcatg aatgcagatc cgtataacgg accagaagcc ccaccgactt
    14161 tcgatatcat cgccatagag aatactttga atgtatcgct gattgtttca ggatgatcgg
    14221 cttgaaaagc ttcttcataa gctgaaaccc ctcttcccat gttatttcca tggtctccat
    14281 cgccaatcgg ggtatctaga tcactgagat attgtttgtt ctgattgatt gcctctgcaa
    14341 attgcgtgag ccattcttgg atttcttgaa cggttagttt catattatca cccttttatc
    14401 ttgagatttt ttaccatgaa atcgtctgta cattgctttc taaagaatct ttccattgtg
    14461 tctccgtcaa gtcgatcagt gttagagaaa gaccttgcat atctagtgag gtcataaaat
    14521 taccgacttt tcggaaaact acattgacgc ctctttcttc caacagagct aaaacatcat
    14581 tcatgaacac gaactgttcc atcaaaggcg tcccgcccat tccgttgact agtacacctg
    14641 cttcttttgg taaattcgag taatcatcta aaatcttagt aacaagttcc tttgccagta
    14701 ctttagaagg ctgcatcttt tctcgacgat agcctggctc accgtggata ccaacaccat
    14761 actcgatctc atcttctcct agttcgaatc ctggtttccc tacttctggt acagttgctg
    14821 ctcgaagcgc tagaccgatc gtcttagttg cctgtacgac cttttctccc agttctttca
    14881 actcagctag ggaagcaccg tttcttgctg catcgcccac gattttgtgt acaaggacag
    14941 tacctgcaac tccgcgtttt ccagcagtat acgtactatt ttcaacggcg atatcatcat
    15001 ctacgatcac actttctact tcaatatcgt ccatagcagc taattccttt gccatatcga
    15061 agttcaatgc atcccctgta taattcttaa cgatcagcaa aactcctttt ccactatccg
    15121 cttccttaat agccgtctga atctgatctg gagttggaga agtaaacaca tcaccaagca
    15181 ccgctgcact taacatccct tcccctacaa acccggcatg ggatggttca tgtccgcttc
    15241 ctccaccaga aaccaaacca acttgttccg ctttctgatt cttggctaca acacgggaag
    15301 attctacacg atgaacaagt tctggatagc tttttactaa tccttccagc atttcttcga
    15361 caatattgct tggatcattc atgatttttt tcaatttatt cccatccttt ctttccggca
    15421 ttttgtgctt ctgttaagat ttgctgagta tcatctgtta taccggcagt gatggctgct
    15481 gcaaaagctc cttccactaa aggcgcatcc accagatgat aacgttgctg ctgctcttct
    15541 tccaacatgt cttttgccag ttccgagttc aataccgcgc taccgatatc cgcaaaaaca
    15601 aaataactag ctgcatcgct tgattcattg actgcttcta cgattttcat cgggtctgat
    15661 ccgatctcgc catttattcc gccgagagaa aaaatttgca cctcttcgga tttttgcatc
    15721 tgctcaatca tttctttaat accatctgtt actttttgac tatgagaaat aagcaaaata
    15781 ctttttttca caagaatctt cctttctttt acatagttgc taacgattgg tttcgctttc
    15841 attaatgaac aattcaagta tataataaat ttctaatttt gggaagatat gtgtagtgct
    15901 tcttgaagtt tgttatactt taattaaaag agaggagtga tcgaatgatt acaacaacaa
    15961 cgaacagtgt agagggaaaa gccgtagtag cttataaagg gatcgttttt ggtgaggtca
    16021 ttactgggat ccatgcattt aaagacttgg aggcggggtt gcgaaatatc tttggcggcc
    16081 gctcaaaaag ctatgaaaac gaactaatgg gtgcaagaga agaagcactt gccgaaatgg
    16141 ccgaacgtgc aaaagcatta ggagctgatg cgatcattgg cgtaaaaatg gactatgaag
    16201 tattaggcag cgacaatggc atgctgatgg tcacatgtag tggaacagct gtctccacga
    16261 gataagtagg ggacatctgt tttaataact aaagagagat gggacatgta gtggcactca
    16321 agaattagat ttttcagtct aacttcgagg tactctacct tcccatcctc tcttttttat
    16381 ttctttttgc ggatgattcc gtaagctgct gcgactaaca cacccgcccc tgctaaggaa
    16441 taactgacag aagctgtttt ttcatttgtt tttggcaaag tctgacttgt tgatttggat
    16501 ggctctgtcg tttgctgaac tgtttctgtc gtgcttccag aggtcactga tttttctcct
    16561 gccgaatgca aaatatcttt tattcgttgg aaactagctg ctaagtcagc tttttttgct
    16621 tcaaaatcat tagcaactgt ttgtgcttcg ctgatctttc cgtggattgc ttgactggcc
    16681 gctgtgatat ccgcttgagt ttgacttagc tttccagaag ctgtttgagc aacctgattt
    16741 aattgctgat tgaattcttt tgcttgttgg atcttcccgt caagatctgt ttggacagct
    16801 tccgcttttg ctttcgcttc ggcaagcttt ttctcaagtt gtgcacggtc atttcccact
    16861 ttatttttaa gatcagctaa atcttgatcg attgctgctt ggtcttcttt caatttgttt
    16921 tttgcagctt ctaatgcttc ttcgagttct tttcttttat tttccaagtc ttgcaaatga
    16981 tcgatttggt cagaagaggt agaactattt attgatgtat cttctgaaga tgaaatcgtt
    17041 tctcctgaat tatctgtcgg tacattggaa gtcatttctg tttccgaggc accgttttca
    17101 gcaccagaag agcttatagt tgttgaaggt tctgtttgag caggttcaga agtttccgca
    17161 aaagctgtct gtcccaataa gaaaatactg gaaacagcaa ttggccagat taacggcttc
    17221 attctttttt tcatcacttt tcccatcctt actacttttt aactaatttc ttattataag
    17281 caaaaatgtt tggaagaacc ataaacacaa caggctgttt gtctaaaaaa cggtgcatct
    17341 tttatttttt tgtacgttcc gggcttttcc atgtcccgct tccataacct aaaatcgttt
    17401 tgactgctgg aatgaaagtc gtcacgatcg ttatcgcgtt cactatccag aacaaaagca
    17461 tatatagtgg tgcgaaaaga aggtatttca attttcttcg tcgatcatct acaatcagcg
    17521 aagctagcaa ctggaaaaat cctgcgacca tttcaaagca gatgaacaca aaactgaatg
    17581 taaaagtaat ataaatctgt tcgtagtttc cttgatatac ataatgcccg attaatccag
    17641 caaaaagaac tacggaaata caaaagaata ttgaccagac aatactcaat gtttgatcaa
    17701 tgaagatgat cgttcttcca atatgtttaa aaggatggag catcactttt ttgaaattcg
    17761 tcagccagac ttctgttcca ccttttgccc aacgttttct ttgtcgatat agcatcttca
    17821 acgtcacagg tacttccatg aaaaagataa tctcaggagc aaaaacagat aaccagtcat
    17881 ttaactgatg atcccaagca atactgatgt cttctgtcgc tcgatcttgt ctgaatccgc
    17941 ccacatcgat caaggcttct tttcgataca tcgtatttgc tccactgtac gcataaagac
    18001 caccgaaaac agctgactgt gttcgtttaa tgatgcccac gatacttgag aattcaaccg
    18061 tttgagattt tgcaatcaat ttggtacgat tttggacatc catatttgca gtgaccgcag
    18121 caatatgtct tgcaccaggg cgaatgaaat aattcacata cctaatcagt gcatctggct
    18181 ccggtaccgt gtctgcatca ttgcttaaaa tcaatttccc ttttgcaaag gcaagtccga
    18241 tattaaacgc gtgggctttt cctttatttt tttcgatccg aaccacacga agattagcgt
    18301 attttttcat cagtctagct aatatttctg gtgtttggtc cgttgaacca tcgtctgtca
    18361 ctaatacctc atagttgtga taattcaatt tcgtcatcaa atactcgatt gtatcttcga
    18421 tcacaatttc ttcattatgg gctggcacca tgatcgtgat gaacggttcg acactcaagg
    18481 gaacatctac ccattctttt tgcttatgtt tgtataaaaa cacataacac cagacaccaa
    18541 taaaccatac aaatccgcct aaaatcggat aagaaacgag aaaaaataca taatagacaa
    18601 acaagtatct aagaataaat tacctgtatg ggtgattgag atcatttcag ttccacccca
    18661 ttttctttat acaaatctgc aatgaatgtc gtatctagat ttttctcttc ggagacagaa
    18721 taaaaacgga ccgtttctct ctcttctttt gttccaaaac gttccgtata cacttcttca
    18781 agtaattctt gtcttttttt caatttttca tcttcatatt caaaagattt atttactttt
    18841 ttgtatagat ttcgattgtt ccacctagtc aatacaaatg aggtaatcag gaaactgaaa
    18901 aaaatcaata caaaaaagcc gattaagaaa tacagcattt tcttttctga taaaaaagcg
    18961 tacaaggaaa aatccttggc tctttctggt ctcgttaatg ggaaaaagac ccatataaaa
    19021 ggaagaacaa cgatcatcca tcctaaaaga gccactaacg tttgccaaat ctttaaccag
    19081 taattacctt ttgaaaaata cttatctttc accaaaattg atgcaacttc ttctcttgtt
    19141 acttttttca tatcctcacc tcacgttttt ttgttacgac gtaggagttt accatacatt
    19201 tcatttcttt tcacataaag tgttcctaaa tttttcctaa gaaaaagaac aaaacatttc
    19261 tcatcagcaa atgaaaaata ttttgttctt tttatcagtt gttcttttta ttttttatct
    19321 cttaattatg ttgctctttc caatacgaca atgcttttca ttcctctaga tatcgccgtg
    19381 tacatttgtc tgcgtcttct ttgctcatct ccatcgtttt caaatggata taagattaca
    19441 tgatcaaatt ccagtccttt tgctagactc aaaggaatga tttggcagtt ttctaatcca
    19501 tatgaatttt ttttaatcga ttcttgtagc gtttgggttt cctcttctgt ttgggtgata
    19561 actgccgctt tttctccttt tagagaaacc aaaatctcag ctagtatttt caacaattct
    19621 tcagaggatc gaaaagagcg gtattcgggt tcttttcctt gcggtcggat cgatacgatg
    19681 tcgatcttct cttgatccac agcataggtt ttgaaaagct ctgtgattgc tccgctagaa
    19741 cggtaactat ttctaagatc ataccgatgg atcggcagat ggtagtcgtc gaaacaacgc
    19801 ataatatcag caaaagtagt ggaagaatta aagattgcct gattctcgtc acctactaaa
    19861 gtaaagtgag tttttggaaa taattcgatc aatagaccta attgtgccag ggtataatct
    19921 tgtacttcat caatcaatag atagcgaaat gctggtacat ttaccttttc aacaagtaaa
    19981 tgacgaatag ctaatataat gactgcttca tcaacagtac gtgtgggttg ccaagcataa
    20041 gctgagccat atcgtttttc ataaattctt tcaaacaact gatgctcatc cacccatgcc
    20101 atttcttcga tttgtctggt tatttttcga tatttttttt gtaaaagttt ccttgcataa
    20161 gctacaatac tttgttccga atcatcagaa atcaatttac cgaataattc caactgccta
    20221 tcttctgtca acgagagtac ctgattctgc acagcaggac tttttgcttg tttcaaaaga
    20281 tgattcttcc attcgctcat cagtgccttt ttcgttgctt gaagcctatc gatcaattta
    20341 ggatgagcag gaactttctc atatattttt tcgatcgttt ttttagaaag gactgttttt
    20401 ccatttttag taagtccacg gataaaattc ggatgatcta aaaacaaagg atctgattgt
    20461 tttagattat cgataaactt tttgctacgc aaacgttctg tctgctccga tacattttct
    20521 cccgtgatac gtttgaaata agcctcttca ccttctattt cctctgctga aagctgtgaa
    20581 accagctgta tgatggttag attcagtgga tttctttcac ctaatgatgg taggacattc
    20641 gaaatatagt cgataaaccg atgattaggt gatagaatca gcaagtcatc ggatgtcatc
    20701 gtatctcgat ataaatagag cagatacgca atacgctgca tgatagcaga ggttttacca
    20761 cttcctgcta cgccatttac caagatattt tgacttttca catcccgaat gattgtgttt
    20821 tgttccgatt ggatcgtagc agtgatagcc ttcatttcat tggtctcatc ttggcctaag
    20881 acttctaata atacgtcatc ttgaatagct acagacgtat caaaaaactt gaccaaacga
    20941 tctctttcca aaagaaattg tcgtctttct ttaataaccg tatcgatttt attttgacga
    21001 accgtataag aagatggacc aatcatgtta ttgtaaaaaa gttcggcaat tggtgaacgc
    21061 caatcataaa caagattttc atctgcatca tctgtgaaat ttgctgtacc gatataaaag
    21121 gattcttttg gctcattatc cagaaagtct acaacgactt ttccaaagta gggacttttc
    21181 aataaacgtt cgatctttgc tagctgattt tctgcgtttt tctgtttgat attcatctga
    21241 tcgatttctc tatttttcat ttcaatcatc gaaaaagtat ccaggttatc taagtaactg
    21301 tcaaaattca atcgaagctc gccagccatt tctttcaacg actggtttcc ttctattttt
    21361 gccgtatcca tcgattcccg cagttcgttt tttttatcaa ttagcttttg atacacgttc
    21421 ttcagatatg cttcttctaa ctgtttttgt gtgttgacca tacacattct cctctttttt
    21481 taaagtggtg gaaccgatta gtttacctct tttaagagtc gaatgcaagc tttttaacga
    21541 gtatcatttg ttaggatata tcagttcttc aggtcggaca gtttgatata aagaaaatgc
    21601 accatcaagg ttttttacgt ggtagccaga ctgcttcaag attctttccg ctagatagct
    21661 acgtaatccg ctatgacagc tgacgatata ttcttgttgg ctgtctaatt catttaatcg
    21721 gtcccgaagt tcattcaaag ggatatgttt ggcttccttg aatttgccct tttccaattc
    21781 ttctgctgtt cgcacatcta ataaaatttt tccttttgct agttctgtcg gcaattcatg
    21841 ccattgtacg gtttcactaa gcccttctgc aatgttcatt gctgcatatc ctagcatgtt
    21901 tactggatct ttggctgagc caaatggcgg tgcatacgtc aattctaatt ccggtaaatc
    21961 gaagatcgtc aaatgccctt tgattgctgt tgctaaaata tcgatccgtt tatccacacc
    22021 ttttgcccca acaccttgtg caccataaat ctcgccagtc tctggatgga agatcaactt
    22081 cagtaaaata tctgttgctt ctggataata gcttgcatga tcttttcctg atacatgaat
    22141 gacttcaaaa gacagttcgt tttctttggc tgttctttca cttaaaccgg tggatgcggc
    22201 agtcattcca aacgttcgta taattgctgt tccaataccg ccttgatttc ttcgagaaat
    22261 tccggaaatc gtgtccgcaa cttgtctacc ttgacgattg gctggtgaag caagagaaat
    22321 aagtgcatct tttccagtga tttgctgttt tacaacgata gcatccccca ccgcataaat
    22381 atcaggaata ttcgtttgat aacgttcgtc tactaggata cccccgcgta tgcctaattc
    22441 gattccagcg gtcactgcta gctggttatc aggacgaaca ccaatggata aaataactaa
    22501 atcagcaggg atggtacgcc catcatccag tcgcaatcgt ttcccgtggt cttcaaatcc
    22561 agcgacagca tgagaagtaa tcacttctac tccttgatga gacaattctt tttctataaa
    22621 agctgccatt tcttcatcca gtgttggaag aatatgtggt gcttgttcga tcaccatgac
    22681 ttctaaacct cttcttttta ggttttctgc catttccaat ccgatgaatc ctgcaccaac
    22741 gatcacggca cgctttggct gtttttctaa tgcatgcatc actttatcga tatctggtac
    22801 atttctaata gaaaaaacat tgtcggcttc tgttatccct ggaatcgatg gaacaactgg
    22861 ttttgcacca ggagataaaa ccaatgcatc gtactgttca atgtgttttt gaccctcatg
    22921 tacgacttct accgttttgt tttcgggaaa gatggctgtc acttcatgat ttggacgcac
    22981 atctaaacaa aaacgagcgg ataatgattc tggcgtttgg acaagaagat tttcccgttc
    23041 actgatttct cccgaaagat aataaggcaa gccgcaattt gcaaaagaaa catacggtcc
    23101 tttttcaaaa acaacgattt ctgcatcttc cattaatcgg cgaagccgtg tagcagcaga
    23161 cattccacct gctacacctc cgacaataac gattttcatc ttatttccct cctactgttc
    23221 gatcaaacca ttgattcatg ccaccacgta cattcactac atcataacca gatttcttta
    23281 attctttagc tgcttgttta ctcctcatcc ctgattgaca aatcacatag acggtctttt
    23341 ctttgtctcc atcatatctg tttatacttt gtaatggaac atttttagct ccttttatat
    23401 gcccgccccg gtattctgca ggtgttctaa catcaagtaa cgctggattt tccaaaagct
    23461 tttcctttaa ttctgggact gttatcgatt ttattgattg aaatagcaaa ttcattttct
    23521 gcacctcatt tacattttta taaatacctc atggggtata ttaaacagga gctttattaa
    23581 tatgtcaacc agaatgactc ttcatttttt tcactacttt ttatatttgc ataaaatacc
    23641 gataaggggt attatctaat tataaaaata gaggagaatg tgttatggag attgataata
    23701 aaaatatcct gaaccgtttg aaacgtacag agggacaaat tcgtggtatc caaaaaatga
    23761 tcgaagacga aaaagaatgt atggatgtca tcactcaatt aagcgctgtt cgttctagta
    23821 ttgatcgcgt aatgggcatg atcgttgcag ataatctgaa aaattgcttt gagaaacctg
    23881 atagtaatcc agaagaacaa gcggctaaac tagaacaagc gatcaagatg attattaaaa
    23941 agtaaaggaa tgatttcaat gaaagtcgtt ttccatattg atgaattaga aaaatggtca
    24001 gaaacaggga aaaacgtaaa aaacctgata aaagcttctc cagaaacgac gattgtcgta
    24061 tctgtaaatg gcatcgcgat taccggttat ctagactctg ccaatgcaga attcttagat
    24121 ttgcaaggag tcgtttttca cgcttgcgcc aatgctatgc gtgctaatca tatttcagaa
    24181 agttctttac ctgaacaagt aatcgttgta ccagcaggtg tccttgattt agttgaactg
    24241 caatcccaag gttatgctta tatcaagcct tagtcagtag aggttgcgac agaagtgatc
    24301 agcttcaaga aatttcggct tatttttcga agaagctact tctggaaaac cgtgaatcaa
    24361 aggttgtgaa aacgctgtcc tgcatcaagt
//
LOCUS       DACEEZ010000036        24036 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20313_2017
            SAMN12639966-rid9879223.denovo.036, whole genome shotgun sequence.
ACCESSION   DACEEZ010000036 DACEEZ010000000
VERSION     DACEEZ010000036.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639966
            Sequence Read Archive: SRR10023158
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 24036)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24036)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741683.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741683.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:44:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,016
            CDSs (total)                      :: 2,962
            Genes (coding)                    :: 2,889
            CDSs (with protein)               :: 2,889
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 36 of 73
            Pseudo Genes (incomplete)         :: 29 of 73
            Pseudo Genes (internal stop)      :: 18 of 73
            Pseudo Genes (multiple problems)  :: 8 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24036
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639966-rid9879223.denovo.036"
                     /isolate="E20313_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(27..725)
                     /locus_tag="IWO89_001475"
     CDS             complement(27..725)
                     /locus_tag="IWO89_001475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylcobyric acid synthase"
                     /protein_id="HAQ0456226.1"
                     /translation="MANYELNIAHLYGNLMNTYGDNGNLLMLKYIAEKMGVSCHTEIV
                     SIHEPFDADKYDLVFFGGGQDFEQLIISKDIQKKKDSLINYIENDGVMLAICGGYQLL
                     GHYYMGANGEKIQGISALDHYTLSQENNRFIGDIVIHNEEFNETYYGFENHNGRTFLG
                     EGERPLGKVVQGQGNNGEDQSEGVIYRNVFGSYFHGPILARNENLAARLIRLALEQRY
                     GKELDLPYLEGQTA"
     gene            complement(718..2070)
                     /locus_tag="IWO89_001476"
     CDS             complement(718..2070)
                     /locus_tag="IWO89_001476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mur ligase family protein"
                     /protein_id="HAQ0456227.1"
                     /translation="MGIRSHFAIAVGKTSQWALKTFFKGGSSLPGKLALKVDPHILDS
                     LAKDYQVVVVTGTNGKTLTTALTVNILRQQFDEVLTNPTGANMVQGIVSTFLQAKPKK
                     GQKKFAVLEIDEASLSKVTEYMKPELFLFTNIFRDQMDRYGEIYTTYKMIVDGAAKSP
                     NATIISNGDSPIFNSIETVNPRKYYGFDHEEDREQMAHYNTDGVLCPNCHHILHYKMI
                     TYANLGKYYCPNCDFKRPELDYRLTDMVRMDNVSADFVIDGHEYGIEVGGMYNVYNAL
                     AATAVAEYFNVDPDKIKQGLGYDEKVFGRQEVININGKKCTLVLVKNPVGINQVIDMI
                     GLAPYPFSLVALLNANYADGIDVSWIWDGNFENFADMDIPAVISGGDRHTDMTLRLKV
                     AGIPENKLTETKELPEVIEKIKELPTEHVYILATYTAVLQLRKELAAQGFIKGGMNRG
                     "
     gene            complement(2293..3693)
                     /locus_tag="IWO89_001477"
     CDS             complement(2293..3693)
                     /locus_tag="IWO89_001477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrkH family potassium uptake protein"
                     /protein_id="HAQ0456228.1"
                     /translation="MHVDLFFRRLQRRGQKFAANHFSSIQIIVFYYILMTVLSLVLFY
                     MPIFREPDSHVSFVDMLFMAISTVSVTGLSTFDINSVFNDNGIILLEILFQVGGLGIM
                     MISTAFVIFSKRRITLKQRQLIMTDMNQPRLSGIVRLIRITFAILIWFQLLFGTFFSI
                     YFYYRGYFDRWRDAVFYGFYQAISAVTNSGFDVTGDSIKPFAHDYFFLILIMFLIFVG
                     GIGFPVLMECREWLLYKRSNAKLPFRFSLFTKLAVLAFVILFVSGTVLIYLLEKDHLF
                     QDSNMSVKWINSMFYSITTRNAGLQIHDLGDFQITTLIIFSLLMFIGCSPSSVGGGIR
                     TTTVAIIGLYLYSFLKSEDNINIFGRRIDQDDVRKSVVVFMLSLGMCFFCIVFLSATE
                     EQTLISIIIEVTSAFGTTGLSLGITGDLSVVGKITIATLMFIGRIGMLYTLMLFVPKE
                     TRDLGYEYPSEKIIIG"
     gene            3854..4591
                     /locus_tag="IWO89_001478"
     CDS             3854..4591
                     /locus_tag="IWO89_001478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="epoxyqueuosine reductase QueH"
                     /protein_id="HAQ0456229.1"
                     /translation="MINAQQIVEKMKNQKINYDTVLKKMIMQWEKEEVKPKILIHTCC
                     APCSTHTLEFMSQYAEITLFFSNSNIHPKSEYLRRLHEQKRFVDQFNEKTDHHVALIV
                     DEYRPNEFTKMVLTNHLENEKEGGARCSACFNMRLDLVAQKAQELGYDYFGSALTISP
                     KKNAALINQIGMDIQKIYGTQYLPSDFKKNKGYERSLQMCKEYDIYRQCYCGCVFAAK
                     QQGCDLKEINREAKAYLKTSTVVFEEN"
     gene            4716..5195
                     /locus_tag="IWO89_001479"
     CDS             4716..5195
                     /locus_tag="IWO89_001479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="A-agglutinin attachment subunit precursor"
                     /protein_id="HAQ0456230.1"
                     /translation="MRKVAPILKPIAVLTISTIVATTTGIVNSSSYRAPSSSSSTVEE
                     TTPTTSVSTSEPTTTESLNETTTTSTWDDTTTSSSYDETYTTDSSSETSNTVPDGTPS
                     DSTNPSTSPDTSASSETIPSSTTVDDNTQNDDAARTAQQNQDSGNTQTYNQQAGDSQ"
     gene            5192..5959
                     /locus_tag="IWO89_001480"
     CDS             5192..5959
                     /locus_tag="IWO89_001480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0456231.1"
                     /translation="MTFLLDLINGVHKFLGYLDISPKYLNRGYTILSVIPTLYLLRIV
                     YGLWQNQNYLQFFLYGLAFLVLVYFTVLNVFYYFLDKNTKADVTQLFVKYLPDEAFNI
                     QTEESIDNGNSIDKVNTQEVMVSYDEDYQLKLAENMRYLIGNGEIKTNELGRMDGFLV
                     DRNTLYPYYYVKKTGEKEYVLNIGRSYSDMEKVGTIRLDSTEDTLEPVGLFIVGGDFV
                     KDGLRYHEPYRLKLIAKKAQSQEEPVVYSRSQRRRHS"
     gene            6189..6758
                     /gene="lepB"
                     /locus_tag="IWO89_001481"
     CDS             6189..6758
                     /gene="lepB"
                     /locus_tag="IWO89_001481"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HAQ0456232.1"
                     /translation="MKEFLKNWGILILVLAAILLARVYVFTPVTVNGHSMDPTLSDGQ
                     RLISSKISNYERMDIITTKEPGDEERMIVKRIIGMPGDTVKMENDQLTINGKKYDEPY
                     LDEFKKEFSEDKLQGEYAYSSGFQAQAESSSTFTSDFEYTVPKGKYLVLGDNRLISKD
                     SRMFGLVDKDMIQGKVVFRYWPLSEIKIM"
     gene            complement(6862..7464)
                     /locus_tag="IWO89_001482"
     CDS             complement(6862..7464)
                     /locus_tag="IWO89_001482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HAQ0456233.1"
                     /translation="MTKNIFDSIANHYDSPDRQALAKIIREELTTYLPRDSHQKVLLD
                     YGGGTGLVSLPLAERFKELIIADASETMLKMAEEKIQAADLKNVRTIHADASVEFPAV
                     QANLILLSLVLLHIPDTENILTKLYEILAPGGQLIIVDFDKNEQISHPKVHNGFSQEE
                     LNNRLEKTGFVSATSHTFHRGEKLFMNKNASLFLSISQKA"
     gene            7570..8039
                     /locus_tag="IWO89_001483"
                     /pseudo
     CDS             7570..8039
                     /locus_tag="IWO89_001483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325408.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
     gene            8145..8729
                     /locus_tag="IWO89_001484"
     CDS             8145..8729
                     /locus_tag="IWO89_001484"
                     /EC_number="2.7.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine kinase"
                     /protein_id="HAQ0456234.1"
                     /translation="MAQLFFKYGAMNSGKTIEILKVAHNYEEQNKPVVLMTSGIDTRD
                     GLGVVSSRIGLKRNAMPIFEETDVFDVIQKMESKPYCVLIDEAQFLKKKHVIAFTRIV
                     DELDIPVMAFGLKNDFRNELFEGSKYLLLYADKIEEMKTICWFCHKKAIMNLHYIDGN
                     PVYEGDQVQIGGNEAYYPVCRKHYFHPSMITEGE"
     gene            8732..9805
                     /gene="prfA"
                     /locus_tag="IWO89_001485"
     CDS             8732..9805
                     /gene="prfA"
                     /locus_tag="IWO89_001485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003128515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HAQ0456235.1"
                     /translation="MYDQLQAIEDRYEELGELLSDPEVISDTKRFMQLSKEEANTRET
                     VEVYRRYKEVVQGITDTEELLGEKLDDEMAELAKEELSELKKEKEVLEEKIKILLLPK
                     DPNDDKNIIMEIRGAAGGDEAALFAGDLFNMYQKYAEAQGWKTEVMEANVTGIGGYKE
                     VIMMITGDNVYSKLKYESGAHRVQRVPSTESQGRIHTSTATVVVMPEAEEVEIDIEDK
                     DIRTDIYHASGAGGQHVNKTASAVRLTHLPTGIVVAMQDERSQIKNREKAMKILRARV
                     YDKIQQEAQSEYDANRKSAVGTGDRSERIRTYNFPQNRVTDHRIGLTIQKLDQILAGK
                     LDEIVDALIMYDQTSKLEAMQNG"
     gene            9798..10637
                     /gene="prmC"
                     /locus_tag="IWO89_001486"
     CDS             9798..10637
                     /gene="prmC"
                     /locus_tag="IWO89_001486"
                     /EC_number="2.1.1.297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HAQ0456236.1"
                     /translation="MGKMTYREVLSRASSFLEDHGKEAYSIQFLFLERKQWKKLDWLL
                     HMNEEISEEEQRLIETDLRLLLADHPPQYLLGYADFYDHRLKVTEATLIPRPETEELV
                     EWCLDETPGVPLEVIDIGTGTGAIAISLKAARKNWHISAVDLSEEALEVAKENAQKEG
                     TKISFYHGDTLGPVMDQQFDVIISNPPYISRNEWELMDESVRSFEPKMALFAENDGLA
                     IYEKIAVEAPSVLKSNGKIFLEIGFRQGEAVKKIFQQAFPDKKVAIKKDLFGNERMIQ
                     VYG"
     gene            10630..10809
                     /locus_tag="IWO89_001487"
     CDS             10630..10809
                     /locus_tag="IWO89_001487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0456237.1"
                     /translation="MVEVVDEKFFICFFIIKKHLFDKDVNDFLVYQQVYPLLTNRLST
                     NSGKLEDKLRQNKNV"
     gene            10831..11856
                     /locus_tag="IWO89_001488"
     CDS             10831..11856
                     /locus_tag="IWO89_001488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonylcarbamoyl-AMP synthase"
                     /protein_id="HAQ0456238.1"
                     /translation="METKRYDETELQEAAEALKEGELVAFPTETVYGLGANALLPNAV
                     KKVFSVKGRPQDNPLIVHVASFEQVKEYVDNFHPAAEKIVKNFWPGPLTLIFKIKKDT
                     LPSVVTGGLSTAAFRIPDNKKTLEVIELSGVPLVGPSANTSGKPSPTTADHVYHDLQG
                     KITGIIDDGATRIGVESTVLDLSDPTAMPMILRPGAVTKEQIEAVIESPVAIDQHLVK
                     ENETPKAPGMKYKHYSPDTRVLMVRDGDWSTAVQWAKNKKIRAGVIASPEIADQVRTD
                     TAAVYMYNDNSVEAAAKGLFAGLRGLDEPTLGLDLIFVQVYPETGLGNAYMNRLKKAA
                     GQNYFEK"
     gene            12031..13275
                     /locus_tag="IWO89_001489"
     CDS             12031..13275
                     /locus_tag="IWO89_001489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HAQ0456239.1"
                     /translation="MVDYKTFDPDLWAAIAKEEERQEHNLELIASENFVSEAVMAAQG
                     SILTNKYAEGYPGHRYYGGCEFVDIVENLAIDRAKELFGAKFANVQPHSGSQANTAAY
                     LALVEPGDTILGMDLSAGGHLTHGSPVNFSGKTYHFVAYGVDPTTEVIDYNVVRILAR
                     KHQPKLIVAGASAYGRTIDFAKFREIADEVGAKLMVDMAHIAGLVAAGLHPNPVPYAD
                     ITTTTTHKTLRGPRGGMILTNDEALAKKINSAVFPGIQGGPLEHVIAGKAVAFKEALD
                     PAFKEYSEQIIANAKAMVKVFNQAIGTRVISGATDNHLMLIDVRELGINGKEAESILD
                     SVNITVNKNSIPFETLSPFKTSGIRIGTPAITTRGFKEEDAVKVAELVVKALQAKDDN
                     AQLDEVKTGVRELTEKFPLHKK"
     gene            13428..14375
                     /locus_tag="IWO89_001490"
     CDS             13428..14375
                     /locus_tag="IWO89_001490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HAQ0456240.1"
                     /translation="MTDRISLEKEAVAFSDANAPHPRIYELAPEEGRVLLEEVQESPV
                     NKYSVDIEDLMVDTGEWGKLNIRLVRPEGNMDKLPVIFYIHGAGWVFGSAQTHDKLIR
                     ELAVRTNSVVVFPEYSRSPEAKYPTAIEQNYAVLQQLNKLAKSKNLDLENLIVAGDSV
                     GGNMATVMTILTKERKGLPISKQLLYYPVTNADFDTDSYHEFGENYFLTREGMMWFWD
                     QYTTNEEERAEITASPLRANINELNDLPSAMILTGEADVLRDEGEAYARKLRDAGVPV
                     TQVRFQGIIHDFVMVNAMDQTKATRAAMALSTSWIKKKE"
     gene            14509..15138
                     /gene="upp"
                     /locus_tag="IWO89_001491"
     CDS             14509..15138
                     /gene="upp"
                     /locus_tag="IWO89_001491"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013774406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil phosphoribosyltransferase"
                     /protein_id="HAQ0456241.1"
                     /translation="MGKFQVIDHPLIQHKLTIIRDKNCGTKVFREVVDEIAMLMAYEV
                     SRDMPLEDIVIETPITETTQKTLSGKKVAIIPILRAGIGMVDGILELIPAAKVGHIGL
                     YRDEETLEPHEYFVKLPEDIDARQLFVVDPMLATGGSAIMAIDALKERGASNIKFVCL
                     VAAPEGVKALQEAHPDIDIYTASLDEKLNEHGYIVPGLGDAGDRLFGTK"
     gene            complement(15277..15519)
                     /locus_tag="IWO89_001492"
     CDS             complement(15277..15519)
                     /locus_tag="IWO89_001492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0456242.1"
                     /translation="MKMATFGKFDTTIVPSEIGEKFKINTHVMFTIHGKSQTGTISKQ
                     LKNSAVVEIDETAENANLMTQSKGVVIVNYKELKKI"
     gene            15640..15712
                     /locus_tag="IWO89_001493"
     tRNA            15640..15712
                     /locus_tag="IWO89_001493"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:15673..15675,aa:Thr,seq:cgt)
     gene            complement(15831..16469)
                     /locus_tag="IWO89_001494"
     CDS             complement(15831..16469)
                     /locus_tag="IWO89_001494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HAQ0456243.1"
                     /translation="MNNILGKKIELKKGILGTIVVVLGLLGLLFAPSIISIFSLTITG
                     FIAFTLVFGKKDTKKMFSKPVAPVKNIAKYFFINVVISAAVSVFLQQILKWGLTGNPI
                     NEAFSPLLFIILPIMILGEELFSVYFLAIFSSKFSIPVASFLSAIIFGFIHYSTYDNG
                     NILHTVAHILLIQGVARLLFNQAAIKSNSIITSWAVHVIFDFTAILLVVLFS"
     gene            complement(16592..17224)
                     /locus_tag="IWO89_001495"
     CDS             complement(16592..17224)
                     /locus_tag="IWO89_001495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HAQ0456244.1"
                     /translation="MIFPKVNEASLNKGSFFHIYENEDFELYNILSQKEVIENQIICH
                     PIIYKSIFKYAFFASNDFQRMEITDCIFENCDFSGCILTKSMLHRVKFVNCKLTGTKL
                     MESYIGNTLFENCKMDYVNLSGSNIKESTFEHSVLSSADFVDCSLTKIMFSTNDLENA
                     SFFDTDLKNIDLSSNSFEMIEVRSEHLKNCTLNQLQALGFARKFLQIKVV"
     gene            17575..17874
                     /locus_tag="IWO89_001496"
     CDS             17575..17874
                     /locus_tag="IWO89_001496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin immunity protein"
                     /protein_id="HAQ0456245.1"
                     /translation="MSKSEEIENMVLKLYDLVLNPEIKEKERILLIDAKTGLEKGQYY
                     PKVINNLERSLRPLAIRGELSKPVSPFYMEISTIGKFEKELGRGMASAPITFGHL"
     gene            18369..20534
                     /gene="acm"
                     /locus_tag="IWO89_001497"
     CDS             18369..20534
                     /gene="acm"
                     /locus_tag="IWO89_001497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="collagen-binding MSCRAMM adhesin Acm"
                     /protein_id="HAQ0456246.1"
                     /translation="MKKCRNIILSMLFIITNITSLIPVHVYADAGRDISSNVTSLTVD
                     PTNITDGGNIKVKFSFDEKKQNIQPGDYLWINWPSEGNIRGEGFQKEIPLMIENKNVG
                     TLTVRKDSAQVVFNENIKNLDSVEGWGQFEIQARNVTDTGEENTGPFTVTSGDKTATV
                     NVTKPASGSSSSVFYYKTGDMLPEDTKHIRWFLNINNNGTYVEQPVKISDEIQSGQRL
                     DPSTFEINQIHLGEQKVYRGEEGIQQFLQDFPSATFNFSVTDNYIEITIPKNFVNLRK
                     IMVSYKTIIENPEQINFENHSEAWFKEFNKPAVDGESFNHTVKNISASGGVNGTVRGE
                     LKIFKYINDTEIGIPNVTFELRRADEQPIQGQSSILLTSNEQGEASIKGLQVGDYVVK
                     EKEAPNWIDFDPLSSNELKFSINENDTEGVSLPIYNKKKVTNITATKIWNGGTTPRPS
                     IYFKLFRASTNNWEPVPDAETKRLDNGITSVTWEDIQQYDDSGNEYTFKVQEVDQNGN
                     DYVPSGYQKIENGLVVTNENKEVISVSGQKTWEDNENQDGKRPTTITVNLLADGQPIQ
                     HKEVSEKDDWRYRFTNLPKYRDGQEIIYTVTEDNVPEYSTTIDGYNIKNSYTPEKTNI
                     SIPGSHITPWKNFPEKTEREKIRNLFSQKLQIFAHTGESSEQRSNYISKRAVPKQITN
                     KSKSILPKTSSKKSSFAVIIGLLIVTICAGIFLQKYKKV"
     gene            complement(20806..21291)
                     /locus_tag="IWO89_001498"
     CDS             complement(20806..21291)
                     /locus_tag="IWO89_001498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl-tRNA synthetase associated
                     domain-containing protein"
                     /protein_id="HAQ0456247.1"
                     /translation="MSRATEQEAYELLKKLGISFQKVDHPAITSVKNVPFSLPGPQVK
                     NLLLKSRKGKQIYLVILPDEKHADLKQLAEQLKEKRLSFVSEEQLPDLLGVPAGTVTP
                     LALMHDKEKKIQVVIDAAIDRQDTVGFHPNINTTTLIMDFSDFEKILVYLEHLPVYEN
                     L"
     gene            21771..22412
                     /locus_tag="IWO89_001499"
     CDS             21771..22412
                     /locus_tag="IWO89_001499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HAQ0456248.1"
                     /translation="MEYLIEVMPQLLVGALTTLKLFLFTLIGSLPLGIVLALGLSLHF
                     KPLRYLLQTYVWVMRGTPLLLQLIFIFYGLPTIGIVFDRFEAALIAFILNYAAYFAEI
                     FRGGIQSIPQGQYEAAQVLQLSPFQTVTKIVIPQVTKIVLPSVGNEVINLVKDSSLIY
                     ILGLGDVMRAGKIAMERDATLLPLLGVAIIYLTLTGILTIIMKQVEGKLNYYR"
     gene            22431..23066
                     /locus_tag="IWO89_001500"
     CDS             22431..23066
                     /locus_tag="IWO89_001500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HAQ0456249.1"
                     /translation="MIELKKVNKAFGEKKIIKDLNLIIPDGKTLAIVGPSGGGKTTLL
                     RILAGLESSDSGEFYLDGVPFNPFELKMKEQVVGVVFQDFQLFPHLNIFENITLAPKM
                     VLKQTKEEYTKKAEELAASLGISDLLNIYPYQLSGGQKQRVAIARALAMNPRVLAYDE
                     PTSALDPELRQQVERLILSLKEDGVTQIIVTHDLVFAENVGDQLLKVSPIH"
     gene            23084..23899
                     /locus_tag="IWO89_001501"
     CDS             23084..23899
                     /locus_tag="IWO89_001501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HAQ0456250.1"
                     /translation="MKKTSFLFALIAGLLLFAGCSNKKTSADNWATINEEKKIVIGLD
                     DTFVPMGFRDKDGKLTGFDIDLAKAVFEQYGITVDFQPIDWSMKEFELNNGTIDLIWN
                     GYSKTPAREKKVQFTKPYMENDQVLITPKESGITNFGQMNNKRLGAQNGSSGYDVFTK
                     QPEVLKEIVKNQDAVLYDSFNEALIDLKSRRVDGLLMDKVYADYYLKQRNELEDFNIT
                     KGSYESEDFAVGARKSDSTLVKKINEALDQLEKTGEFQKISDKWFGEDVTPRN"
BASE COUNT         8319 a         4090 c         4778 g         6849 t
ORIGIN      
        1 ttgtcacatt cccctttttc gaataactag gctgtttgtc cttcaagata gggcagatcc
       61 aattctttac cgtaacgttg ttctaaagct aggcggatca aacgagctgc caggttctca
      121 tttcttgcaa ggataggtcc atggaaataa gaaccgaaga catttcggta aatgacacct
      181 tcagattggt cttcaccatt atttccttga ccttgtacca ctttccccaa aggtcgttca
      241 ccttcaccta agaaagtccg accattgtgg ttttcaaatc cataataagt ttcattgaat
      301 tcttcgttat ggataacgat atcaccgata aaccggttat tttcctgact taacgtgtag
      361 tgatccagtg cactgattcc ttggatcttt tctccattag ctcccatgta gtagtgacct
      421 agcaattggt agccaccaca aatggctaac atgacgccat cattttcgat atagttgatc
      481 aaagaatctt tctttttctg gatgtctttt gaaatgatta attgctcaaa gtcttgacca
      541 ccgccaaaga aaaccaagtc atatttatca gcatcaaaag gttcatgtat actaacgatc
      601 tccgtatggc aagaaacccc cattttttct gctatatatt tcaacatcaa aaggtttccg
      661 ttatcaccat atgtgttcat caaatttcca taaaggtggg cgatattgag ttcataatta
      721 gccacggttc attcctcctt tgataaatcc ttgagcagct agttctttac gaagctgcaa
      781 tacagcagtg taagtagcta aaatatagac atgctcagtt ggcaattctt tgattttttc
      841 gattacttct ggtaattctt ttgtttctgt caatttattt tctggaatcc cggcaacttt
      901 tagacgtaaa gtcatatctg tatgacggtc tcctccagaa ataacagctg ggatatccat
      961 atcggcaaag ttttcaaaat taccatccca aatccagctg acatcgattc cgtctgcgta
     1021 attggcattt aataaagcca ctaaagaaaa tggataagga gctagaccaa tcatatcgat
     1081 tacttgattg atacctacag gatttttgac aagtactaat gtacattttt tcccattgat
     1141 attgatgact tcttgtcggc cgaatacttt ctcgtcatat cctaaacctt gtttgatttt
     1201 atcaggatcg acattgaagt actctgccac tgcagtagct gctaaagcat tgtagacatt
     1261 atacataccg ccaacttcga ttccgtattc atgaccatcg atcacgaaat ctgctgacac
     1321 attgtccatc cgtaccatat cagttaatcg ataatccaat tcaggacgtt tgaagtcaca
     1381 atttggacag taatatttcc ctaagtttgc ataagtgatc attttataat gcaagatgtg
     1441 atgacaattt ggacataaga caccgtctgt attgtaatga gccatttgtt cacgatcttc
     1501 ttcatggtca aagccataat attttctagg attgactgtt tcgatagaat tgaagattgg
     1561 tgaatcaccg ttactgataa tcgttgcatt tggtgacttt gcggcaccgt ctacaatcat
     1621 tttataggtc gtatagattt ctccataccg atccatttga tcacggaaaa tatttgtaaa
     1681 taagaacagt tctggtttca tatattctgt gactttgctc aaacttgctt catctatttc
     1741 taggacagca aactttttct gtcctttttt tggttttgcc tgcaagaaag tcgatacaat
     1801 tccctgtacc atattcgcac cagtagggtt ggttagaact tcatcgaatt gctggcgcaa
     1861 aatattgact gttaaagcgg tagtcaatgt tttaccgttt gttccggtga caacgacgac
     1921 ttggtagtct tttgccaaac tatctagtat atgtggatcg actttcagcg ctagtttacc
     1981 tggtaaactt gatccgcctt taaaaaaagt ttttaatgcc cattgcgacg tttttccaac
     2041 tgcaatcgca aaatgactgc gtattcccat tatgtaacct ccaaaaacga tttgctgacg
     2101 aataaattca actaatcata ccatatgttg aaaaaaaggt taaattgctt ttcttattct
     2161 taagaaaagg ttacggctga aagtgcatga aatcggacaa aatcgcatga tttaacaaaa
     2221 aaacagcttg aaacaaaatg attgtttcaa gctgctgtga ttttattatt ccctcttacc
     2281 tatattccca ttttaaccga taataatttt ctcgcttgga tattcatagc ctagatctcg
     2341 tgtttctttt ggcacgaata acatcaatgt atatagcatt ccgatccgcc caataaacat
     2401 cagtgtagca atcgtgattt tgcccacaac agacaagtct ccggtaatcc ctaaagataa
     2461 accagttgtt ccaaacgcag aagttacttc gatgatgatc gagatcagcg tttgttcttc
     2521 tgtagcagat aaaaaaacga tacaaaagaa acacatacca agagaaagca taaagacaac
     2581 gactgatttt cgcacatcat cttgatcgat tctccgtcca aaaatattga tgttatcttc
     2641 acttttgagg aaggaatata gatacaaacc gatgattgct acagtagttg ttcgaatccc
     2701 gccgcctaca gaactaggac tgcatccgat aaacatcagt aaagaaaaaa tgatcagtgt
     2761 tgtgatttga aaatcaccca aatcgtgaat ctgcagacca gcattacgag tcgtaatcga
     2821 ataaaacatc gaattgatcc atttgacact catattggaa tcttggaata aatggtcctt
     2881 ttccaataga taaatcaaga cagttcccga aacaaacaag ataacaaaag ctaacacagc
     2941 taatttggta aacaaggaaa aacgaaacgg aagcttcgca ttggaacgtt tgtataatag
     3001 ccattcccgg cactccatca atacaggaaa accaattcct ccgacaaaaa tcaaaaacat
     3061 gatcaggatc aagaaaaaat agtcatgtgc aaagggcttg atggaatccc cagtgacgtc
     3121 aaacccagag ttcgtgacag ccgatatcgc ctgataaaaa ccgtaaaaaa cggcatcacg
     3181 ccaacgatca aaatagcctc tgtaataaaa ataaatagaa aagaatgtcc caaaaagtaa
     3241 ttgaaaccag attaaaatag caaaagtgat ccgaatcagt cggacgatac cagacaaacg
     3301 cggttgattc atatccgtca tgatcagctg tcgctgtttc agtgtgatcc gtcgtttaga
     3361 aaaaatgaca aaagcagtag aaatcatcat gatccccaaa ccaccaactt gaaataatat
     3421 ttccagcagt ataataccgt tatcattgaa cacagaattg atatcaaaag tcgatagtcc
     3481 agtcacactc actgtactga ttgccatgaa cagcatgtcc acaaaagaaa catggctatc
     3541 tggttcgcga aaaatcggca tataaaatag aacgagtgat aagaccgtca tcaatatata
     3601 ataaaaaacg atgatttgga tcgatgagaa atgattggca gcaaacttct gtcctcgccg
     3661 ctgcagcctt cgaaagaata aatcaacatg cacagtacta tcctcctgac aatagtcgtt
     3721 tctaccagta tacaagggga attaggaata ctcaaagaaa ttatcaaaca gataacaata
     3781 cagctttcga ttaaaataga aatggcttat aataaggaaa atcctagaaa aaagaaggag
     3841 gaggaataac aaattgatta atgctcaaca aatcgtagaa aaaatgaaaa atcaaaaaat
     3901 aaattatgat acagttctga aaaaaatgat catgcaatgg gagaaagaag aagtaaagcc
     3961 taagatatta atacatacat gctgtgctcc ttgcagtacg cacacattgg aattcatgtc
     4021 tcaatatgca gagatcacgt tatttttttc aaattcgaat attcatccta aaagtgagta
     4081 tttgcgaaga ttacatgaac aaaaacgatt tgtagaccaa tttaatgaga aaactgacca
     4141 tcatgttgcg ctaatcgttg atgaataccg tccaaatgaa ttcaccaaaa tggttttaac
     4201 aaaccatctt gaaaacgaaa aagaaggtgg tgctcgctgc agtgcatgct tcaatatgcg
     4261 attagatctt gttgcgcaaa aagcgcaaga actaggatac gattactttg gaagtgccct
     4321 gaccatctcg ccaaagaaaa atgcagcttt gatcaatcag atcggaatgg atatccaaaa
     4381 gatttacggg acacaatact tgccaagtga tttcaagaaa aacaaaggct atgaacgctc
     4441 tttgcaaatg tgcaaagagt atgatattta tcgccaatgt tattgtggtt gcgtgttcgc
     4501 tgccaagcag caaggatgcg atctgaaaga aatcaatcgg gaagcaaaag cttacttaaa
     4561 aacatcaacc gtagtttttg aggaaaatta aagaaatctt tcggaaaaac gtaagaaaaa
     4621 aataaagaat ttatggtaga aataaggtag actgtataat ttgtccgtag taaagtaagg
     4681 aaacaagaat aaatttcaat gaaggaagag ataagatgcg aaaagtagca cctattttga
     4741 agccaatcgc tgtattaaca atctcgacga ttgtcgcgac tactacaggt attgtgaata
     4801 gttccagcta ccgtgcacca agttcaagtt ctagtactgt agaagaaaca acaccaacta
     4861 cttcagtctc gacatctgaa ccaacaacaa cggaaagttt gaatgaaacc actacgacaa
     4921 gtacatggga cgatacaaca actagttcgt cgtatgatga gacatataca actgacagct
     4981 caagcgaaac aagtaatacg gtaccagatg gcactccatc ggattcaacc aatccaagta
     5041 caagtccgga tacaagtgct agctccgaaa cgataccaag cagtacgact gtagatgata
     5101 acacgcaaaa tgatgatgca gcacgtacag cacagcaaaa ccaagattca ggaaataccc
     5161 aaacgtataa ccagcaagca ggtgattctc aatgacattt ttactagacc ttatcaacgg
     5221 ggtgcataaa ttccttggct atttagacat cagtcctaaa tatcttaacc gtggatatac
     5281 gattttaagt gtgattccca cgctttattt attgagaatt gtttatggac tttggcaaaa
     5341 ccaaaactat cttcaatttt tcttatatgg acttgcattt ctagtgcttg tttattttac
     5401 tgttttaaat gtattttatt actttttaga taaaaacacg aaagccgatg tcactcagct
     5461 ttttgtgaag tatttacctg atgaagcgtt caacatccag acagaagaat cgatagataa
     5521 tggaaattcg atcgataaag tgaatacaca agaggtcatg gtttcttatg acgaagatta
     5581 tcaattgaaa ttagccgaga atatgcggta tttgattgga aatggtgaga tcaaaacaaa
     5641 tgaactcggt cgcatggatg gatttctggt cgatagaaat acattgtatc cttattacta
     5701 tgtcaagaaa acaggagaga aagagtatgt tctaaatatt ggacgaagct actcggacat
     5761 ggaaaaagta gggaccattc gacttgactc aacagaagac acattggaac ctgtcggatt
     5821 gttcatcgtc ggcggtgatt ttgtcaaaga cgggttacgc tatcatgagc cttatcgtct
     5881 gaaattgatt gcaaaaaagg cacagtcgca ggaagaacca gtggtttata gtcgtagcca
     5941 aagaagaaga cattcataat gtgatgaaac gccgtgtatc agtggttgcg ataaaaacgt
     6001 ttattcggaa taagggggat gtgacacgtc ttttgtcacg ttcctttttt atataacgag
     6061 aaagataaga cgaacacatc acttattctt tcctttcatt tataaacatt gtagaataaa
     6121 gaaagtacaa cgaagtattg tgaaaaaacg taataaataa cgaggaaaat acaagtagga
     6181 ggtaagctgt gaaagaattt ttgaaaaatt gggggatttt gatccttgta ttagcggcta
     6241 ttttattagc tagagtgtac gtgtttacac cagttactgt aaacgggcat tcgatggacc
     6301 ctacattaag tgatggacaa cgattgattt catctaagat ctcaaactat gaacgaatgg
     6361 atattattac tacaaaggaa ccgggggatg aagaacggat gattgttaaa cggatcatcg
     6421 gtatgcctgg agatacagtc aagatggaaa atgaccagtt gaccattaat ggcaaaaagt
     6481 atgatgaacc ttatctggat gagttcaaaa aagaattttc agaagataag ttacaaggag
     6541 aatatgctta tagctcagga ttccaagcac aagcagaaag ttcatccacc tttacgagtg
     6601 actttgaata tactgtgcca aaaggaaagt atcttgttct aggcgataac cgcttgattt
     6661 caaaagacag ccgaatgttt gggttagtcg acaaagatat gatccaagga aaagttgttt
     6721 tccgttattg gcctctttca gaaattaaaa tcatgtgatg aaaaaataaa aagaagctgt
     6781 gacatgtttt ttgtcacagc ttctttttcc gaaaattcct tcttatttct tggagttgct
     6841 cacttttttt gcaatctcat tttaagcttt ttggctgatg gataagaaaa gcgaagcgtt
     6901 tttgttcata aatagctttt ccccacgatg gaaggtatga ctagtagcag agacaaagcc
     6961 cgttttttcc aatcgattat tcaattcttc ttggctaaag ccattatgga ctttcggatg
     7021 actaatttgt tcattcttgt caaagtctac gatgatcaat tgaccgccag gagctaaaat
     7081 ttcataaagt tttgtaagaa tattctccgt atcaggaatg tgtaaaagta caagtgatag
     7141 aaggatgaga ttagcttgga cagcaggaaa ttcgacagaa gcatcagcgt ggatcgttcg
     7201 gacatttttc agatctgctg cttggatctt ttcttctgcc attttcagca tagtttctga
     7261 agcatcggca atgatcagtt ctttgaatct ttccgcaagc ggtaaactaa caagcccagt
     7321 tccgcctccg taatccagca ggactttttg gtggctgtcg cgcggtagat aagtagttag
     7381 ttcttcgcga ataatcttgg ctaatgcttg acggtcaggg gaatcataat ggttagcgat
     7441 ggaatcaaaa atattttttg tcatcaaata gctccttacg taaaactttg aaattttaac
     7501 ttattatagc acgtgaaaag aaagaaagca tgctatactc aattcaaaaa gggagaatct
     7561 agaaagaata tgccgatatc gtttgtaaaa gatagagagg aaaaaggaaa gtgtgtccga
     7621 gaaatattac tagacttgcc tgagtggttt gggcttcctg aatcaacgga aaaatatatc
     7681 gaggaatcaa gtaaactacc tttatggtgt gaaaaaagaa aagaagaata tttaggcttt
     7741 atcactcttt cacagacaag tgaagatact gccgaaatct attctattgt atgggaataa
     7801 aaaaagacta tcatcaccaa ggattaggaa caaaactttt taaagaagca gaaggttacg
     7861 cagcaaaaca ttacaaatat ttgcaagtga agactgtaga cgaggggcac tactctatat
     7921 atgatcaaac tatttgtttc tacgaatcat taggattttc aaggttggaa gtttttccaa
     7981 atttatggga tgaatggaac ccatgtttag ttcttgtcaa aaaattggaa caaaagtgag
     8041 caatagattt aatactaatc tgtgcttgat gaaaattctc actgtaaaaa atgctgaaaa
     8101 tcaagtataa taccatagga acaaactgga aggtgaaaaa gagcatggca caactatttt
     8161 ttaagtatgg cgcaatgaat agcggcaaga cgatcgagat cctaaaagtc gcacataatt
     8221 atgaagaaca aaataagcca gttgtcttaa tgacaagcgg tattgataca agagatggtt
     8281 taggcgttgt ttcaagccga atcggactga aacgtaatgc gatgccgata tttgaagaaa
     8341 cagacgtatt tgacgtaatt cagaaaatgg aaagcaaacc ctattgtgtg ctgatcgatg
     8401 aagcgcaatt cttaaaaaag aaacatgtca ttgcttttac gagaattgtg gacgaattgg
     8461 atattccggt tatggcattt ggtttgaaaa atgattttcg aaacgagtta tttgaaggat
     8521 caaaatattt attgctttat gcagacaaaa tcgaagaaat gaaaacaatc tgttggttct
     8581 gccacaaaaa agcaatcatg aatttacatt atattgatgg aaaccctgtt tatgaaggcg
     8641 atcaagtgca aattggcgga aatgaagctt attaccccgt ttgtcgaaaa cattactttc
     8701 acccatcaat gattacagaa ggagaataag catgtacgat caattacaag caattgaaga
     8761 ccgttatgaa gagcttggag aacttttaag tgacccagaa gtgatttctg atacaaagcg
     8821 ttttatgcag ctttcaaaag aagaagcaaa cacgcgcgaa acagtcgaag tttatcgtcg
     8881 ttataaagaa gtagtacaag gaatcaccga tacagaagaa ctgcttggtg aaaaactgga
     8941 tgatgaaatg gctgaactag caaaagaaga attatctgaa ttaaaaaaag aaaaagaagt
     9001 cttagaagaa aaaatcaaga tcttgctttt accaaaagat ccaaatgacg ataaaaatat
     9061 catcatggaa atccgtggag cagcaggcgg agacgaagca gcattattcg ctggcgatct
     9121 tttcaatatg taccaaaagt atgctgaagc acaaggctgg aaaacagaag tgatggaagc
     9181 taacgttacc ggaatcggtg gatataaaga agtgatcatg atgatcaccg gagacaacgt
     9241 atattcgaaa ttaaaatatg aaagtggtgc gcaccgcgtc cagcgtgttc cttcaactga
     9301 atcgcaaggg cgtatccata cttccaccgc aacagttgtc gtgatgccag aagctgaaga
     9361 agtagagatc gatattgaag acaaagatat ccgtacagat atctatcatg cttcaggtgc
     9421 tggcggacag cacgtcaata aaacagcatc tgctgtacgt ttgactcact taccaacagg
     9481 gatcgttgtt gctatgcaag atgaacgttc tcaaatcaag aaccgtgaaa aagcaatgaa
     9541 aatcttgcgt gctcgtgtgt acgataagat ccagcaagaa gcacaaagcg agtatgatgc
     9601 caatcgaaaa tcagcagtcg gaactgggga tcgttcagaa cggatccgga cgtataattt
     9661 cccgcaaaat cgtgtgactg atcatcgtat cggattaact atccaaaaat tagatcagat
     9721 tttagcaggg aaattagatg agatcgttga tgcattgatc atgtatgatc aaacatcaaa
     9781 actggaagct atgcaaaatg ggtaaaatga cctatcgaga agtcctgtca cgggcttctt
     9841 cttttttaga ggatcatggg aaagaagcat atagtatcca atttttattt ttggaacgaa
     9901 aacaatggaa aaaacttgat tggctccttc atatgaacga agagatttca gaagaagaac
     9961 agcggttgat cgaaaccgat ctgcgacttc tgcttgcaga tcatccacct cagtatttgc
    10021 taggatatgc agatttttat gatcatcggt taaaggtaac tgaagcaaca ctgatccctc
    10081 gaccagaaac agaggagtta gtcgaatggt gtttagacga gacgcctggt gtaccgttag
    10141 aagtgataga tattgggaca gggactggag caattgctat tagtttgaaa gcagcaagaa
    10201 aaaattggca tatatctgct gtggatcttt cagaagaagc attagaagta gcgaaagaaa
    10261 atgctcagaa agaaggaacg aaaatcagtt tttatcatgg agatacatta ggaccagtaa
    10321 tggatcagca atttgatgtg attatttcta atccgccgta catcagtcgg aatgaatggg
    10381 aactgatgga tgaaagtgtc cggtcattcg aacctaaaat ggcccttttt gctgagaacg
    10441 atggattagc tatttatgaa aaaatagcag ttgaagcacc aagcgtacta aaatcaaatg
    10501 gaaagatttt tttagaaatc ggatttcgcc aaggtgaagc agtaaaaaaa atatttcagc
    10561 aagcatttcc agataaaaaa gtggcgataa aaaaggatct ttttggcaat gaacgaatga
    10621 tccaagttta tggttgaagt tgtggatgaa aaatttttta tttgtttttt tattataaaa
    10681 aaacacttat ttgacaagga tgtaaacgac tttttggttt atcaacaagt ttatccacta
    10741 ttgacaaata gattatccac aaacagtgga aaacttgagg ataaactcag acaaaataag
    10801 aatgtatgaa aaaggggttg tggataaact gtggaaacga aacgatatga tgaaacagaa
    10861 ttgcaagagg cagccgaagc actgaaagaa ggagaacttg ttgcttttcc taccgaaaca
    10921 gtatacggat tgggcgccaa cgctctttta ccaaacgctg tcaagaaagt gttttctgta
    10981 aaagggagac cgcaagataa tccattaatc gtccatgtcg cctcttttga acaagtaaaa
    11041 gagtatgtgg acaactttca tccggcagca gagaagattg tgaagaactt ctggcctgga
    11101 ccattaactt tgatatttaa gatcaaaaaa gacactttgc cctcagttgt gacgggagga
    11161 ttatccacag ctgcatttcg tataccggat aacaaaaaaa cactagaagt aatcgaactt
    11221 tccggtgttc cgcttgtcgg tccaagtgca aatacgtcgg gaaaaccaag tccaacgact
    11281 gcagatcatg tatatcatga tctacaagga aaaattaccg ggattattga tgatggagct
    11341 acgcgaattg gtgtagaatc cactgttctt gatttaagtg atccaacagc catgcctatg
    11401 attttacgac caggtgcagt aacgaaagaa caaatcgaag cagtcatcga atctcctgtt
    11461 gctattgatc agcatcttgt aaaagaaaat gaaacaccaa aagcccctgg gatgaaatac
    11521 aaacactact cacccgatac acgtgttctg atggtcagag atggcgattg gtcaactgca
    11581 gttcaatggg caaaaaataa gaagatccgt gcaggtgtca tagcaagccc cgaaattgcg
    11641 gatcaagtgc ggacagatac tgcagctgtt tatatgtata acgacaactc agtagaagcg
    11701 gcggctaaag gattgtttgc aggtctaaga ggattagatg aaccaacact tggattagat
    11761 ttgatttttg tacaagtgta tccagaaact ggattgggga atgcttacat gaatcgcttg
    11821 aaaaaagcag caggacaaaa ttattttgaa aaatgatcaa ttggttattg tcgcattctt
    11881 ttagtatgat acaatgctat caagtaaatc ttctatcagt gaacgaaaaa ctaagcgtaa
    11941 ctaatcgtaa tagaagagat acgaatattt ggcaatttgt ttttgatggg aaaaataaaa
    12001 ttgtaggaaa ataaaaagga gctgttgttt gtggtagatt acaaaacgtt tgaccctgat
    12061 ctttgggctg cgatcgccaa agaagaagaa agacaagaac ataatttgga gctgattgcc
    12121 tctgaaaact ttgtctccga agcagtgatg gctgcacaag gaagtatctt gacaaataaa
    12181 tacgcagaag gttatccagg gcatcgctat tatggtgggt gtgaatttgt cgatatcgtt
    12241 gaaaatttag cgattgaccg tgcaaaagaa ttattcggag cgaaatttgc gaacgtccag
    12301 cctcactccg gctctcaagc caacacggct gcttatctcg ctttggtaga accaggggac
    12361 acgatcttag gaatggatct ttccgcaggc ggacatctaa cacatggatc accagtcaac
    12421 ttcagtggca aaacctatca ctttgttgct tacggtgttg atccgacaac agaagttatt
    12481 gattataatg tggtacgtat tttagctaga aaacatcaac caaaattgat cgtagcaggt
    12541 gccagtgctt acggacgaac gatcgacttt gcgaaattca gagaaatcgc agatgaagta
    12601 ggcgcaaaac tgatggtaga tatggcacat atcgcaggat tagtagcagc aggacttcat
    12661 cctaatccag ttccttatgc agatatcaca acgactacca ctcataaaac attgcgtggc
    12721 cctcgaggcg gcatgatttt gacaaacgat gaagcattag caaaaaaaat aaatagtgcg
    12781 gtatttcctg gtattcaagg cggccctcta gagcatgtga ttgcgggtaa agcagtagca
    12841 ttcaaagaag cattagatcc tgcatttaaa gaatatagtg aacaaatcat cgccaatgca
    12901 aaagcaatgg tcaaagtttt caatcaagca attggaacca gagtcatttc aggggcgaca
    12961 gataatcatc tgatgttgat cgatgttcgc gagttaggta taaatggaaa agaagcagag
    13021 agcattttgg acagtgtgaa tatcactgta aataaaaact cgattccttt tgaaacacta
    13081 agtccattca aaacaagcgg tattcgaatc ggcacgccag caatcactac tcgcgggttc
    13141 aaagaagaag atgctgtaaa agtggctgaa ctagttgtca aagcattaca agcaaaagat
    13201 gataacgctc aactagacga agtcaaaaca ggtgtacgcg aattaactga aaaatttccg
    13261 ttgcataaaa aataaaaatt taaattccat tagtaaacag aaaagttcaa tgttgggctt
    13321 ttctgtttat tttttgggaa aacggattct ttgacaatat cggtgatgct ctctagtata
    13381 tgaggtgtaa ttttagtctt cacactaaat ctcagggagg aaaagtcatg actgatcgta
    13441 tctcgttaga aaaggaagca gtagcattca gtgatgcaaa tgcgcctcat ccgcgtatct
    13501 atgaactagc accggaagaa ggaagagttt tactagaaga agtacaagaa tcacctgtga
    13561 acaaatattc cgtcgatatt gaagacttga tggtggatac tggcgaatgg ggaaaactca
    13621 atattcgtct cgtacgacca gaagggaata tggataaatt accagttatt ttttatattc
    13681 atggagcagg ctgggttttt ggcagtgctc aaacccatga taaattgatc agagaacttg
    13741 cggttcgtac gaattccgtc gttgtctttc cagaatatag tcgatcacca gaagcaaaat
    13801 atccaacagc aattgaacaa aattatgctg tgttgcaaca gctgaataaa ttagccaagt
    13861 caaaaaatct ggatctcgag aatctaatcg ttgcaggaga ttcagtaggt ggtaacatgg
    13921 ctacagtcat gacgatctta acaaaagaac gaaagggcct cccaatatcc aagcaattgc
    13981 tgtattatcc agtcacaaac gctgattttg atacagattc ctatcatgaa ttcggagaaa
    14041 actatttttt gacaagagaa ggaatgatgt ggttttggga ccaatatacg acgaatgaag
    14101 aagagcgtgc tgaaatcaca gcttcgcctt tgagagcaaa tataaatgaa ctaaatgatc
    14161 ttccttcagc aatgatttta acgggagaag cggatgtatt aagagatgaa ggtgaggctt
    14221 atgcgcgcaa acttcgtgat gcaggggttc ctgttacaca agtacgcttc caaggaatca
    14281 tccatgattt tgtcatggta aatgctatgg atcagacaaa ggcaacgcgt gcagcaatgg
    14341 cattatctac ttcttggatc aagaaaaaag aataagcttt gcttgtccgc aggaaaactt
    14401 cttttcaaaa acggattttc ttaggaaaaa tggcgagttt gactagtcaa tcacttgatt
    14461 ttgttttaca attaaaaaga aacgacagaa caaaggagag cttaaaaaat gggcaaattt
    14521 caagtgatcg atcatccatt gattcaacac aaactaacta ttattcggga caaaaactgc
    14581 ggaacaaagg tttttcgtga agtagtcgat gaaatcgcta tgttgatggc atatgaagta
    14641 tcacgggata tgccattgga agacatcgtg atcgaaacac cgatcactga aacaacacaa
    14701 aaaacgttgt ctggtaaaaa agtagcgatt attccaatct tgcgcgcagg tattggaatg
    14761 gttgacggta tcttggaatt gattccagct gctaaagtgg gacatatcgg tctttatcga
    14821 gatgaagaaa cattggaacc acatgaatat ttcgtcaaac ttccagaaga cattgatgca
    14881 cgtcagttgt ttgtagtaga tccaatgttg gcaacaggcg gatcagcaat catggcaatc
    14941 gatgcattga aagaacgtgg cgcgtcgaac attaaatttg tttgtttggt agcagcacct
    15001 gagggagtaa aagctttgca agaagcgcat cctgatattg acatctatac agcttctcta
    15061 gatgaaaaat tgaatgaaca tggttatatc gttcctggac taggggatgc gggtgaccgt
    15121 ttgtttggta caaaataagt aaatacgaaa aagggtgtga cacaactttt gtcacatcct
    15181 tttttggtca tttttaacgt ttgatgcaga acagcttttt cacagctttc gtttgcatat
    15241 aaaaaaacca tcccaaaaat gcagagatgg tcaaattcaa atctttttta gttctttgta
    15301 gttaacgatt actacaccct tactttgcgt catcaagttc gcattttctg cagtttcatc
    15361 aatctcaaca acagcggaat ttttcaattg ctttgaaatc gttccagttt gcgacttgcc
    15421 gtgaattgtg aacattacgt gagtattgat cttgaatttt tcaccaattt ccgatggtac
    15481 gatagttgta tcaaattttc caaaagttgc cattttcata aaacctgcct ttcacatcaa
    15541 ttataccacg ttttatgaaa aatatcatga aattaaacta attttccatc ggactcttta
    15601 caatcagcca aatagagtat ataatttctt ttggataagg ccagcttagc tcagttggta
    15661 gagcaacgca ctcgtaacgc gtaggtcgta ggttcgatcc ctatagctgg cattaaaaga
    15721 gaagacagat atatcaagga tttttaatcc attgatacat ctgtcttttt gtttaaagga
    15781 aataaagtat gctttcttag caatagagca tgtattattt ctataaaaat ttaggagaat
    15841 aaaacgacta gcaatatagc tgtgaaatca aatatcacat gaactgccca acttgtaatg
    15901 atcgagttgc ttttaatggc agcttggttg aataacaatc ttgccacccc ttgtatcaaa
    15961 aggatgtgtg ctacagtgtg taaaatattt ccattatcat aagtactgta atggataaat
    16021 ccaaagatga ttgctgacaa gaaactagcg acaggaatgc tgaacttact agaaaaaata
    16081 gcgagaaaat acactgagaa caattcttct ccaagtatca tgataggcaa aataataaat
    16141 aataacgggc tgaaagcctc atttattgga ttgcctgtta aaccccattt caaaatttgt
    16201 tgcaaaaata ccgagactgc ggcactgatt actacattga taaaaaaata tttggctata
    16261 tttttgacag gagcgacagg tttcgagaac atttttttcg tatctttttt accaaaaaca
    16321 agtgtaaatg ctataaatcc agtgatagtt aaagaaaaaa tgctaataat ggatggcgca
    16381 aatagcaacc ctaacaaacc gagtacaacg acaatagtgc ccaatatccc ttttttaagt
    16441 tctatttttt ttcctaaaat gttattcatg ataataactc ctactattca gtttttgaag
    16501 atagagtcag tatacatgaa gagttaaaat aggcaatgtt tttttatgga tatttgtatt
    16561 gctaaaaatt tatcttatat attcattttg attatactac ttttatttgt aaaaattttc
    16621 tagcaaaacc aagtgcttgc aactgattaa gcgtacaatt ttttagatgt tccgagcgta
    16681 cttcaatcat ttcaaaggag ttgctactta aatcgatatt ttttaaatca gtatcaaaaa
    16741 agctagcatt ttctaaatca ttagtagaaa acattatttt agtgagggaa cagtctacaa
    16801 aatctgcgct tgataagaca gagtgctcaa aggtactttc ttttatgttt gaaccagata
    16861 aatttacata gtccatttta caattctcga ataacgtgtt cccaatataa ctttccatca
    16921 atttagtacc tgttaattta caattgacaa atttcacgcg atgcaacatg ctttttgtca
    16981 atatacagcc tgaaaaatca cagttttcga atatacagtc ggtaatctcc attctttgaa
    17041 aatcattgga agcaaaaaat gcatacttga aaatagattt gtaaataatt ggatggcaga
    17101 ttatttggtt ttcgatgacc tctttttgtg aaaggatatt atacagttca aaatcttcgt
    17161 tttcatagat atgaaaaaag gatcctttat tcaagcttgc ttcattaact ttaggaaaaa
    17221 tcatatttaa ttacctccat tttcataaag tcatattaac aaaagctaaa ccaacttaca
    17281 ttagcagaaa atgagatagg ttttcgcctt tttcgataac gatagtgtcg ctatctcttg
    17341 aaacgttctt agaattgccc tgagagacac aaattaggcg taattctaat aaaaatctta
    17401 gaataaaaat ttttactgat ttaatttaga atgtttttaa aataaaaatc gttgccataa
    17461 cagtgtattg aaaggtttta acaatccagt aatgatttaa aaaaatctag atgtagcgta
    17521 tactttgttt atagaatgta gtgggaagaa atatattttt gggaggaaat cgtgatgtct
    17581 aaaagtgaag aaatagaaaa catggtacta aaattatatg atctcgtttt aaatccagag
    17641 atcaaagaaa aggaacgtat acttttgata gacgcaaaga caggcttaga aaaaggtcag
    17701 tattatccta aagtcataaa taatttggaa cgtagtttac gtccgttagc aatcagggga
    17761 gaattgtcta aaccagtatc accgttttat atggagatat caacaatagg aaaatttgaa
    17821 aaagaattag gtcgaggaat ggcttcggca ccgattactt ttgggcattt ataaaaacga
    17881 gttattcatc tcaatgaatt aaaaagtata gtcaatctca taatgcaggc taggttcatt
    17941 tatttgaaca gagtctgtat ttttttattc taaataaaga aacagatgtt tctttatatt
    18001 gtattttatc ctttaattaa cacaaaaaca gctagtataa actaataatg ttaattaaaa
    18061 gtgtatttta tgaaactatc gttatttatt aatgttgcat ttttgtaagt taaaaattgg
    18121 aaatatagat tgaaaaaatg aaagcaataa gtaaaataaa atgaatatat gcgaaaaaat
    18181 aatttacatt aggaaaaacc ttgtaaaaac agttttttgg catatttatt ttttataaat
    18241 atttgtaaaa taactatcaa ttttttagtt aaaagattgg aaatgagtgg gggaggaatt
    18301 gaagaaagga tttttgagag atgatatagt aggttttatt tttagttaaa ataggggaga
    18361 tagaaaaaat gaaaaagtgt agaaatatta ttttatcaat gttatttatt attactaata
    18421 taacatcact gattcctgtt catgtctatg cggatgcagg cagagatatc agcagtaatg
    18481 tcacttcgtt gaccgtggac ccaacgaata ttacagatgg aggaaacata aaagtaaaat
    18541 tctcgtttga cgaaaaaaaa caaaatatcc aaccaggtga ttatctttgg atcaattggc
    18601 caagtgaagg aaatattcgc ggtgaaggat tccaaaaaga aatcccatta atgattgaaa
    18661 ataaaaatgt tggaacttta acggtcagaa aagattctgc gcaggttgtg tttaatgaaa
    18721 atattaaaaa cctagactca gtagaaggtt gggggcaatt tgagatacag gccagaaacg
    18781 taaccgatac cggtgaagaa aatacgggac cattcaccgt gacgagcggt gataaaacag
    18841 ctactgtaaa tgtgacaaag ccagcttctg gttcttcttc tagcgttttt tattataaaa
    18901 caggagacat gctacctgaa gatactaagc acattcgatg gtttttgaat attaacaata
    18961 acggcactta tgttgaacaa ccagttaaaa tcagtgatga aatccaaagt ggacaaagat
    19021 tagatccaag tacgtttgaa attaatcaaa tacacttagg tgaacagaag gtttatagag
    19081 gagaagaggg aatccaacaa tttttacaag atttccccag cgctacattt aatttttcag
    19141 taactgataa ttacattgaa ataactatac caaaaaactt tgtcaatctt aggaaaataa
    19201 tggtgagtta taagacgatc atagaaaacc cagaacaaat aaattttgag aatcattcgg
    19261 aagcttggtt taaagaattt aataaacctg ctgttgatgg agaaagtttc aatcatacag
    19321 ttaaaaatat ttcagcttct ggtggagtca acggtacagt tagaggcgaa ttgaagattt
    19381 tcaaatatat caatgacaca gagattggta tcccaaatgt gactttcgaa ttaagacgag
    19441 cagatgaaca gcctattcaa ggacagtcta gtatcctgtt gacttcgaat gaacagggag
    19501 aagctagtat caaaggcctg caagtaggag actatgttgt aaaagaaaaa gaagcaccca
    19561 attggattga ttttgatcca ctaagttcaa atgaactcaa attctccata aacgaaaatg
    19621 atacagaggg agtcagttta cctatttata acaagaaaaa ggttacgaac atcacagcaa
    19681 ctaaaatttg gaacggtgga accactccaa gaccgagtat ttattttaaa ttattcagag
    19741 caagtacaaa caattgggaa ccagttcctg atgcagaaac aaaacgatta gacaatggaa
    19801 taacttcagt gacttgggaa gatatccaac agtatgatga tagtggaaac gaatatacgt
    19861 ttaaagtgca agaagtagat caaaacggaa atgattatgt cccttcaggt tatcaaaaaa
    19921 ttgaaaacgg gctagtcgtt acaaatgaga ataaagaagt catttcagtc tcaggacaaa
    19981 aaacatggga agacaatgag aaccaagacg gcaaacgacc gacgacgatc acagtaaatc
    20041 tattagccga cggtcaaccg atccaacaca aagaagtaag tgaaaaagat gattggcgct
    20101 atcgtttcac aaatctgcca aaatacagag acgggcaaga aatcatctat actgtaacgg
    20161 aagacaacgt gcccgaatac agtacgacga ttgacgggta taacatcaaa aacagttata
    20221 caccggaaaa aacaaatata tctataccag gtagccatat aacaccgtgg aaaaattttc
    20281 cagagaagac agagagagag aaaattagaa atctattctc tcaaaaattg caaatatttg
    20341 ctcatacagg agaatccagc gaacaaagaa gcaattatat tagtaagaga gctgtaccta
    20401 aacagataac aaataaatca aaaagcattc ttcctaaaac aagtagtaaa aaatctagtt
    20461 ttgctgtaat cattggttta ttgatagtaa ctatctgtgc tgggatattc ttacaaaaat
    20521 ataaaaaagt ttagttagaa tataatattt tttgttaaat atgactgaaa tcaaaagaat
    20581 aaaattccga tttataatcg aaaaaagaag catttttata tatgcttctt ttttcgtaga
    20641 atcaataaat atggatttgg ctaactatgg aagaatactc gtattagttt tcagcgttca
    20701 tactagttgt tacattgttt gattggccaa aagaatttct gatatatttt gtgctaaata
    20761 aagaaaaaga cgcttgaatt gggaaataag aaaactactc tatcattaca aattttcata
    20821 aactggcaga tgttctaagt aaacaagtat cttttcaaag tcggaaaaat ccataatcaa
    20881 cgtagtggta ttgatatttg gatgaaagcc tacggtatct tgacgatcaa tagcagcatc
    20941 gatcacgact tggatttttt tctctttgtc gtgcatcaat gctaatggag tcactgtacc
    21001 tgctggaacg cctaggaggt caggaagctg ttcttcagaa acaaaagata agcgcttttc
    21061 ttttaattgt tcagctagtt gttttaaatc ggcgtgcttt tcatcaggca agatgacaag
    21121 atagatttgt tttccttttc ttgatttcaa taatagattt tttacttgcg gaccaggtag
    21181 ggaaaaaggg acatttttga ctgacgtaat cgcaggatga tcgacttttt gaaaagagat
    21241 acccagtttt tttagtagtt cataagcttc ttgttcggtt gcacgactca tcgcgcatca
    21301 ccctcctttt tttattagtg tatcataaaa aagagttgac aaatgaaaaa taatcatgta
    21361 aattacacgt aaagttaaaa caaatgacat cgaaaagaag agtagccgtt aggtgttttt
    21421 agagagtctc tggttggtgg aaagaggcag cagttaaccg tgaaacacat cttggagtcg
    21481 attttctgaa gcgtatagta ggaaaatccg gggagcccgt tacagctgcg gtcgttgcta
    21541 gatgaccaga taaggctggc ttttccgtga ggagtcggta aaaaaaggtg gaaccacgtg
    21601 attaacgtcc tttgatcttc ggatcaaagg actttttgtt ttcatctgaa cggcaacgac
    21661 tgtgtgaggt tttttctgca aggaaaaaat aaacagaggt ggaaccacgt ataatacgtc
    21721 ctcttggcat ttgccaagag gattttttta ttttaaagga ggaaaagaag atggagtatt
    21781 tgattgaagt tatgccgcaa ttattagtag gagcactcac aacattgaaa ttatttttat
    21841 ttaccttgat tggctcatta ccattaggga ttgttttagc attaggattg tctttgcatt
    21901 ttaagccgct acgctacttg cttcaaacat atgtatgggt tatgcgggga acaccgctgt
    21961 tgttgcaact gatttttatt ttttatgggt tgccgacgat tgggatcgtt tttgatcgtt
    22021 tcgaagcagc actcattgcc tttatcttaa actatgcagc ttattttgca gagatctttc
    22081 gtggagggat acaatcgatt cctcaaggtc aatatgaagc agcgcaagtt ttgcaattaa
    22141 gtccctttca aacggtgacg aaaatcgtca tcccacaagt gacaaagatt gttttacctt
    22201 ctgtagggaa tgaagttatc aacttggtca aagattcttc cttgatctat atattaggtt
    22261 taggtgacgt aatgcgtgct gggaaaatcg cgatggaacg cgacgcgact ttactgcctt
    22321 tgctaggcgt ggcaatcatc tatcttacat tgactggtat tctaacaatc ataatgaaac
    22381 aagttgaagg aaaattaaat tattaccgct aactgatagg aggaagaaat atgattgaat
    22441 tgaagaaagt gaacaaagca tttggtgaaa aaaaaataat caaagatttg aatctaatca
    22501 ttccagatgg gaaaacatta gctatcgtag gtccttccgg tggcggtaaa accacgcttt
    22561 tacgtatctt ggctggctta gaaagttctg acagcggaga attttattta gacggtgtac
    22621 cgttcaaccc attcgagctt aaaatgaaag aacaagttgt aggagtcgta ttccaagact
    22681 tccagctatt tccacatctg aatatttttg aaaacattac cttagcacca aaaatggtat
    22741 tgaaacagac aaaagaagaa tacaccaaaa aagcagaaga attagcggct tcattaggga
    22801 ttagtgattt attgaatatc tatccttatc agctttctgg cggacaaaaa cagcgggtag
    22861 cgattgccag agcattggct atgaatccac gggtattggc ttatgatgaa ccaacaagtg
    22921 ctttagatcc tgaattacgc cagcaagtcg agcgtttgat cctatcattg aaagaagatg
    22981 gggtaaccca aattattgtc acccatgatt tagtatttgc agaaaatgtc ggtgatcaat
    23041 tactaaaagt ttccccaatt cattaagaaa ggagaagata gagatgaaaa aaaccagttt
    23101 tctattcgct ttgatcgcag gtcttcttct ttttgcaggc tgttcgaaca aaaaaacttc
    23161 agcagataat tgggcgacta tcaacgaaga gaaaaaaatc gttataggtt tagacgatac
    23221 atttgttcct atgggattta gagataaaga cgggaagctc acaggatttg atattgattt
    23281 agccaaagca gtctttgaac aatatgggat tacggtagac tttcagccca ttgactggtc
    23341 aatgaaggaa tttgaactga acaatggaac gatcgatttg atatggaacg gctattccaa
    23401 aacgccggca agagaaaaga aagtgcaatt tacaaaacct tatatggaaa acgatcaagt
    23461 gctgatcaca cctaaagaat caggaatcac aaactttgga caaatgaata ataagagatt
    23521 aggtgcgcaa aatgggtcat ctggttatga tgtatttacg aaacaaccag aagtattaaa
    23581 agagatagtc aaaaaccaag atgctgtttt gtatgatagt tttaatgaag cattgatcga
    23641 tctgaaaagc agacgggtcg atggtctgct gatggataaa gtatacgcag actattattt
    23701 gaaacaaaga aacgaactag aagacttcaa cataacaaaa ggttcttatg agagtgagga
    23761 ttttgcagtt ggtgcacgca agtcagatag tacattagtg aaaaaaatca atgaagccct
    23821 agatcaacta gagaaaacag gtgaattcca aaaaatatcc gataaatggt ttggcgaaga
    23881 tgttacgccg agaaattaac aaatagggga ctgtgacaaa agacgtgtca cagtcccttt
    23941 ttccgaataa acagtgttct cctgccaact ccttgtgact cggagctgaa tggcaggctc
    24001 acaacctctt ggtaaacggt gttcaaaagc cagttt
//
LOCUS       DACEEZ010000037        23834 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20313_2017
            SAMN12639966-rid9879223.denovo.037, whole genome shotgun sequence.
ACCESSION   DACEEZ010000037 DACEEZ010000000
VERSION     DACEEZ010000037.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639966
            Sequence Read Archive: SRR10023158
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 23834)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23834)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741683.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741683.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:44:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,016
            CDSs (total)                      :: 2,962
            Genes (coding)                    :: 2,889
            CDSs (with protein)               :: 2,889
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 36 of 73
            Pseudo Genes (incomplete)         :: 29 of 73
            Pseudo Genes (internal stop)      :: 18 of 73
            Pseudo Genes (multiple problems)  :: 8 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23834
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639966-rid9879223.denovo.037"
                     /isolate="E20313_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(171..476)
                     /locus_tag="IWO89_001502"
     CDS             complement(171..476)
                     /locus_tag="IWO89_001502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1292 domain-containing protein"
                     /protein_id="HAQ0456251.1"
                     /translation="MTEHNHSHDHEGHEHITLVDDQGNETLYEILLTVDGQEEFGRNY
                     VLLYPAGVSEDEDVELQAYAYIENEDGTEGELEQIETDAEWDMIEEVFNTFMAEEEE"
     gene            complement(501..932)
                     /gene="ruvX"
                     /locus_tag="IWO89_001503"
     CDS             complement(501..932)
                     /gene="ruvX"
                     /locus_tag="IWO89_001503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HAQ0456252.1"
                     /translation="MRVMGLDVGSKTVGVAISDPFGWTAQGVEIIRINEAEEEFGFDR
                     LKELVAQYEVDRFVVGLPKNMNNSIGPRAEASMSYGEKIKELFSLPVDYQDERLTTVQ
                     AERMLVEQANTSRAKRKKVIDKLAAVMILQNYLDGSGKQLF"
     gene            complement(929..1195)
                     /locus_tag="IWO89_001504"
     CDS             complement(929..1195)
                     /locus_tag="IWO89_001504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IreB family regulatory phosphoprotein"
                     /protein_id="HAQ0456253.1"
                     /translation="MGFTDETVRFGFDDSRKKEVSETLAIVYRALEEKGYNPINQIVG
                     YLLSGDPAYIPRYRDARNLIRRHERDEIMEVIVKDYLSNHGVNL"
     gene            complement(1450..2175)
                     /locus_tag="IWO89_001505"
     CDS             complement(1450..2175)
                     /locus_tag="IWO89_001505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA/RNA non-specific endonuclease"
                     /protein_id="HAQ0456254.1"
                     /translation="MSKKTTKAIVGIVAAVIAGAFGVSTTQKDSDIFQQITTIFDSTP
                     QASQVADVPEYDGKHQEIEINGNQPEFTKEDLSLSKGTWESYSNIDKLNRVGQANAML
                     GKDLFPTEKREPLYIDPTGWKQKKLSDGQWLYNRSHLIGFQLTGQNNNIKNLMTGTRS
                     LNAPYMLAHENDIAAYIKETNHHVRYRVTPHFEGNELVARGVQLEAESIEDKKIEFNV
                     FIYNVQDGYTINYETGQATKTKN"
     gene            complement(2536..2606)
                     /locus_tag="IWO89_001506"
     tRNA            complement(2536..2606)
                     /locus_tag="IWO89_001506"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2572..2574),aa:Gly,seq:ccc)
     gene            2723..3475
                     /locus_tag="IWO89_001507"
     CDS             2723..3475
                     /locus_tag="IWO89_001507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter family protein"
                     /protein_id="HAQ0456255.1"
                     /translation="MTGKELDLLLDTCLLAGKIMMESNAEMYRVEDTMSRIALASGNF
                     RLVSYVTQTGLFIGLDRTSTIRMEQITNRSINLEKVVKVNDLSRKYVASELTLEELYC
                     ELQAIDRDRSFFPVWLQIVSAAVISGTIMVLFGGKMTDLPVTLVIGGLGYAAYLYSLK
                     FFRIKFLSEFLSSLLIGIAAIYSVRFGLGSNQDLIIIGCVMPLVPGVQITNAIRDLLA
                     GHYVSGVSRGTEAMMTATMIGFAIAFIFQLFY"
     gene            3491..3970
                     /locus_tag="IWO89_001508"
     CDS             3491..3970
                     /locus_tag="IWO89_001508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter"
                     /protein_id="HAQ0456256.1"
                     /translation="MGTIFIQFSFSFLATAAFAVITNVPRRSLICCGLSGAFGWMIYW
                     ICVQIGGSAAFGSLLGSLGVAFISDLFSKRLKMPVTIFNIPGMVPLVPGGLAYQAVRN
                     LVTESYREAAHYAVQAIMIAGAIALGLVLSEVFNHNIRNFKVKRDAIMMKRKKRLFK"
     gene            complement(4221..5801)
                     /locus_tag="IWO89_001509"
     CDS             complement(4221..5801)
                     /locus_tag="IWO89_001509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter"
                     /protein_id="HAQ0456257.1"
                     /translation="MYKRQLLELIRVNLLYVNPQLTNKAREKGKKGKRLTIYLLSQNL
                     FSGLIFLLIYGFAMFAMNFVKLPGFFTYYVALFGMLGLTQSISVIYNIFFEGNDLQVF
                     LPLPFGQEQVFSAKILVVALTIIPFVFPLLVMFILTGWRADLGILLTIIISILLFSLF
                     LIILFCLASLIVFGLARTAVFKKHRKTVSSILLGSSMLIAFAGIMWMNHQTGTIENEL
                     TDRHPISILLPFYDAASQPLSQQGLLGWGILAITALGFFLAVKQLIVPKLTEQLTDSN
                     SEFKIKRTHKKNQNVHQLLFSYNAQLVKEPNLIMQVLSSSLLTPIIFILAFALGGEIK
                     LTDLDNKLIGVVFLVGIALAFLTVNQTSFISNLISLDQENFLFIRSLPISMNQYLKEK
                     FRFGWILQSFLTGGIALLSGLSFQLPLFFIPSLLFGSLFGCYLLSLRYFARDYRLLLL
                     NWTNINQLFNRGSGSLGLVATMMGSLIISIILIVLYGMLALFYPFWLVNIPVIGLVLI
                     LSALWIVYYQRTFWKKFE"
     gene            complement(5794..6537)
                     /locus_tag="IWO89_001510"
     CDS             complement(5794..6537)
                     /locus_tag="IWO89_001510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ0456258.1"
                     /translation="MIEFINVSKSYGKKQALKDLSLSIRQGEIFGFLGHNGAGKSTTI
                     KSLVSIIEPSSGTILVDGMKLTENRLSIKQKIGYVPDSPDIFLQLTAGEYWDLISAAY
                     ELDTQKKEKRLAELTALFDMYSHQNETIASFSHGMRQKTILIGTLLPDPDIWVLDEPL
                     QGLDPQAAFDLKEMMKAHAAKGKTVIFSTHVLDTAQQLCDKLAILKKGELIYQGAVSE
                     LLNGSPDETLEKIYLKMAGRQATGDGPYV"
     gene            complement(6713..7489)
                     /locus_tag="IWO89_001511"
     CDS             complement(6713..7489)
                     /locus_tag="IWO89_001511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAQ0456259.1"
                     /translation="MVTRIFILDDSLVFEKMLVEILEESRILLSPWKFEIVQGLNVIQ
                     FVEFIKNNEIYSSDIFFLDIDLNAYYSGIELGEMLREKNEKCKIVYLTSHPNKAITVI
                     NRKITPSGYLIKEFTYEKNKKSIQSYLLKEKENYPVQHATKKRWTTVRYGNSDHYIHY
                     DEILYLMSMPGYKNKIGLVLKDEEILVNGTIKKYKKSLEYEYLCKDLKAYIINTENIT
                     SISRVNEIVTFANGEQLEMGKRSIDKLKNFMQEKIENESF"
     gene            complement(7737..8573)
                     /locus_tag="IWO89_001512"
     CDS             complement(7737..8573)
                     /locus_tag="IWO89_001512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03943 family protein"
                     /protein_id="HAQ0456260.1"
                     /translation="MLRFLILSGYFELMMYLQVSGKLDQYINVHYRYLAILSMILSAL
                     LALVQLYLWVKNGKAEEKAHEHDHDHGLSKPYQRGIAYVLLALPVIVGTLFPTVSLDT
                     TIVEAKGFNFPVSKESVGDPEMQTQYLKPDTSIYFNKSDYLDQMNELKEKYGDKQTIK
                     ITDENYLEVMELIYNYPSEFIGKKISYEGFVYQTPNDSNTDIFLFRFGIIHCVADSGV
                     FGLLTHMPEGVTVKNDEWYKIEGTIQSDYYQPFKREIPSVVVTNAEKVSAPKNQYVYR
                     SF"
     gene            complement(8584..9513)
                     /locus_tag="IWO89_001513"
     CDS             complement(8584..9513)
                     /locus_tag="IWO89_001513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HAQ0456261.1"
                     /translation="MFSFIPNSVLQMSTIFLSIVIEALPFVLLGCLISGALQVFLTPE
                     RVKRWLPENRIFSIFTGSILGFFFPSCECGIVPIVHQFVKKGVPVHTAFAFMLTAPII
                     NPIVIFSTFIAFGNSWQMASWRVIGSFFVALVIGVWLAYFQKDAVLKEKITSKVAENH
                     HHHVHEEEQSEKNEPKNQLIHQTGHVLTHSIDEFFDTGRYLIIGGLIAAAMQTYLPTR
                     IMLTLGSTKILAIIIMLLLAFTMSLCSEADAFIGSSLLSLFGTAPVAAFLVFGPMVDI
                     KNLLMMKRYFTGRFILSLIGLIALSVIGFTLFI"
     gene            9705..10058
                     /locus_tag="IWO89_001514"
     CDS             9705..10058
                     /locus_tag="IWO89_001514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HAQ0456262.1"
                     /translation="MKQTTNLTEVQDILQQSSVAVIYLSMPNCSVCHAVRPRLEELLT
                     HYDIPALHLDAFETPEVASKFEVLTAPVVLIFHQGKEVFRQARFIDFKKIRYLLDELT
                     AEDDSLSYEEIFRTK"
     gene            complement(10132..10788)
                     /locus_tag="IWO89_001515"
     CDS             complement(10132..10788)
                     /locus_tag="IWO89_001515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensing transcriptional repressor Rex"
                     /protein_id="HAQ0456263.1"
                     /translation="MKDHIIPKATAKRLPLYYRYLRILNNAGKKKVSSTELSEAVQVD
                     SATIRRDFSYFGELGKRGYGYDVEDLMHFFAKTLNDDQLTNVALIGVGNLGSALLKYK
                     FHQSNSIRISCAFDVNEEIVGRIVDGIPVYPMEDMSEQIKIQQIEVAILTIPAERAQE
                     VVNQLTEAGIKGILNFTAARLNAPSDVLIQNVDLTNELQTLIYFLHSDANAHSENTAE
                     "
     gene            11014..12948
                     /locus_tag="IWO89_001516"
     CDS             11014..12948
                     /locus_tag="IWO89_001516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HAQ0456264.1"
                     /translation="MILLQANQIARHFGAEVLFENIHLEISTGARVALVGRNGAGKST
                     LLKIIAGIEAPDEGKIAKNKTATLGYLAQDTGLDSNETVWNEMLKAFEQVRQMEERMR
                     ELEIFISEGTPDTPAYSSYLKEYDRLQHDFSDRNGYGYENEIRSVLHGFKFDSSFYDQ
                     KIDTLSGGQRTRLALARMLLQHPDILILDEPTNHLDIETLSWLENYLQSYTGALLIVS
                     HDRYFLDKVVNEVYELSRRKMRHYKGNYSKYLDLKAEQLASEWKAYEKQQTEIHKLED
                     FVARNLVRASTTKRAQSRRKTLEKMDRLDRPQGDEKQANFLFSIEKPSGNVVLQIEDA
                     AIGYDNVTLSEPINLDIRRQEAIALVGPNGIGKSTLLKSIIHQIPFIRGKETLGTNVT
                     IGYYDQGQADLHTNKTILAELWDEHPTTPEKEIRSALGAFLFSGEDVEKPIPLLSGGE
                     KARVALAKLAMNKENFLILDEPTNHLDIDNKEVLENALIDYDGTLLFVSHDRYFINRI
                     ATKVVELSENGSKVYLGDYDYYLEKKQEEEEIAELLAQDEPESAPAPTNKSDYYQSKE
                     QQKLIRSLQRKITQVEEEMARIDEWIDQLEQEMVAPENLEDHVKLNELNQDLEAARQD
                     QETKLTEWEELSLELEEYEN"
     gene            complement(13034..14257)
                     /locus_tag="IWO89_001517"
     CDS             complement(13034..14257)
                     /locus_tag="IWO89_001517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronyl hydrolase"
                     /protein_id="HAQ0456265.1"
                     /translation="MLKAGGEMLDKVMFEEIIHPEKWINECYKELMEKEWNNEIERCR
                     RHTERLIKRISNSQYQFPSECPIKGRYPLRDNIGWTEGFWTGINWLLFEKTGDPLFQQ
                     TAEKADIDFIHRIDCQIITNHHDLGFLYSLSTVADYKITGNRKMKEASIDAAETLLQR
                     FWEKAGVIQAWGDPSDPSQQGRMIMDCCMNLPLLYWASEETGNQKYKKAAQAHIQNAA
                     DYLVREDGSTYHTFYMDVKDGKPRYGSTHQGYSDNSCWARGQAWGIYGFALSYRYLKE
                     QKYFDLSKILANYFMNRLPEDTICYWDLIFDDSSREEKDTSSMVIMVCGLLELIESGL
                     LDKTEKNLYQSAVWKIMRQLKERYATTEEEDGFLTQSVYNKPKNNGVNTPCIWGDYYY
                     LEALVRMTSNWSSYW"
     gene            complement(14251..16149)
                     /locus_tag="IWO89_001518"
     CDS             complement(14251..16149)
                     /locus_tag="IWO89_001518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2264 domain-containing protein"
                     /protein_id="HAQ0456266.1"
                     /translation="MVVKRGENTMKHPVPLLRTRQDYFDLFKKINQPLQPFYRASGAQ
                     IDYIHQGVGYGNKIAGMEGFSRVLWGAGPAIDQLDERWLKLILSGIKAGTDPSHPDYW
                     GEIHERDQRMVEMPAMLLALYHHEQLLWKKLDTGEKSQIIHWFSQIFEYECADGNWQF
                     FKIIVGTIIQKFGYPVKESALKEAFDNIENCYLQNGWYRDSSRGRQDYYNPFAFHYYG
                     LIYSVLEPEKPISQIYKNRAIDFSRDYIHFFAEDGANVPFGRSMIYRFAVSSFWSAML
                     WADLLPFKLGEIKGLVNRNIRWWMQKEIFDEKGILNIGYTYPQLTLTEPYNSTLSPLW
                     LNKIFLLLALPEDHEYWTAKEMPMPIREGTKLLSEANLLIQHDNGHTFFLNAGQLGPN
                     FHTLTNEKYLKFAYSSQFGFSIPRANQLKAEMAMDSMLGIQRADTYITTSFKNESIKT
                     YGQFIVRNNVRDIVCKKEVLASTWEPTLQAKIRTWLIPFEGWQIRVHKVELDTEYVLY
                     ETGFAIECSPEKEGIIIEEDKENYRVSEAGFSGIICLSDDTIKRKSTAIMGLPNTNLM
                     TWENTFIPGLEGTFGKGTHWLGTGVVAHQDRDYSLEVWNNRPKIDFDGTTGLTIQNKN
                     NKKRLKLC"
     gene            complement(16143..17261)
                     /locus_tag="IWO89_001519"
     CDS             complement(16143..17261)
                     /locus_tag="IWO89_001519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase family 88"
                     /protein_id="HAQ0456267.1"
                     /translation="MNEQTKEWSRKILNVASEKIDAELDRMGNKIPYISYNGKYTDVI
                     KDRGIDWWTNGFWSGILWQLFHKTKREKFKEAAIQQEQLLESALYHFSDVHHDVGFLF
                     LPSAIAHYQQSQNKHSRQLGLHAATVLAGRYNISGNYLVAWNDHKPGWMIIDSMMNLP
                     LLYWASRETGDPRFSKIARQHADTTAKYLVRKDGSTGHIAEFDAESGKYIRLLGGQGY
                     NENSCWSRGNAWALYGFALSYRYTKNPYYLAIAEKIAVNFIQKIEKTGYIPLIDFAVK
                     DSNIDTSAGVCAACGLLELAGYVSSVRSKMYHEAAINILQKTEKHYANWNMDEDGIIG
                     GGAEAYHRELTYNVPLIYSDYFFIEGLLRLEQSELAIW"
     gene            complement(17344..18177)
                     /locus_tag="IWO89_001520"
     CDS             complement(17344..18177)
                     /locus_tag="IWO89_001520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HAQ0456268.1"
                     /translation="MTKQKIQSRIFTYVIIVFGALIFLMPYLYMIVASTQSNQEILSG
                     GINFKFGDMLLKNWQAIMARYNYPLIIWNSIVIAVISTVLATASSTMAGYALAKYRFM
                     GNKLLFNTVMLSRMVPFFTTLIPLFYIMSRLGLSNTYAGVIIPSIASTTSVFMMRQYS
                     SQFPTALMEAARIDGANEWVIFTKIAVPILSPTIITSGLLIFMGSWNQYLFPLVMLSD
                     KDKYTVPLIIKNMTVSSTGEPINYGAIMLILATSVIPMVLIYVWAQSKFKENDIGAAN
                     K"
     gene            complement(18170..19087)
                     /locus_tag="IWO89_001521"
     CDS             complement(18170..19087)
                     /locus_tag="IWO89_001521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAQ0456269.1"
                     /translation="MMQSSLPSKTIKNSKKKATDTSHKKSAYGLVTPFVIFFIVTILI
                     PIIISFAFSFMKIGYSFEFVGFDNYLAIFRDPLFIKSYTNVLIIMAGSIPITMLLAIY
                     FAVLLNSAQLKGKGFFRTVYYIPTITSTVAVASVFMTFFNPTGLFNGFLNALGINGVQ
                     WLTNPFWIRISMIITMIWLSVGYNTILFLAGLQGISKEIYESAEIDGASKFRQFFLLT
                     IPMLKPIILMATVLATINGLGNFNIPNIFFGTSNGPENSALVVGVNLYKTSFEMVDFG
                     KASAIAWTMVFVSVILSAIQFKFGGDKND"
     gene            complement(19314..20438)
                     /locus_tag="IWO89_001522"
     CDS             complement(19314..20438)
                     /locus_tag="IWO89_001522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HAQ0456270.1"
                     /translation="MKKRYKLVIALLNITLLFSLSACGKTNEEVTANIADPMIKKATT
                     LEVYVANGKLTNYVENAAKIYNRKTGANIDLKIVNVASGTATVQMITPKLVTHEDMPD
                     IISMADTSAAGVLEKFQDAFYSADEYGFYDKYGDQFYDQKLNILRQQTSNETIIPWAS
                     DFTPALCYYQPKKFAEIGVDFEEISSWEEYIQVAKALKEKTGSFGIALPESGDQELFI
                     NMLAQQNQSLLDEEGNIRLNTKEAKHAAKLIKEMIDSDIVHFYGAQDAEKAFQESAMF
                     VAGGWYATNMSLNFPDASDKWRMAPLIPFSKENPGKGAVSGGSSFYVPKDAKNPQVAQ
                     QFLTFMLTDEECLANALELGVATSNSLAYQTEAAEKKFDY"
     gene            20621..21529
                     /locus_tag="IWO89_001523"
     CDS             20621..21529
                     /locus_tag="IWO89_001523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAQ0456271.1"
                     /translation="MVLLLTNNQSSNLVKKTLTYSIDTHLHLSAAPYFRLLINESANL
                     YAEFAHKSIELDNPSLLFIDKQQEVQIFSTQKETQTFIIIQFYFDEQLLAHITGQSFS
                     IDQEITSIITFLKNEQFKIDYLKHSKPAISYDQEINSFFILCQQMSIHLLSLLVTYCL
                     QKIQLSSLPRHYQQELALLTIPSNEKVIPKKKHILYSDELVDPIIRYLEEHLSENFLL
                     EQLAQIFFISPASLKKHFRKVNGKSVISYFKELKMEKAKRLVLENELTYTEIADILGF
                     HSIHHFSSAFKKYTGLSPSKYYLSLK"
     gene            21670..23283
                     /locus_tag="IWO89_001524"
     CDS             21670..23283
                     /locus_tag="IWO89_001524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-disulfide reductase"
                     /protein_id="HAQ0456272.1"
                     /translation="MKVVVIGGVAGGPSFATRFRRLNEAHEIIIYERGENISYASCAL
                     PYYLGGVITDRDSLIERTPEILKTKNNIDVFTKHEVTAIDPSTKRLTVKDLSTNEEIK
                     TDYDKLIISSGARPDYPDIPGVFEAENGFVLRSVTDADRIKSFLEEKNPQHVVILGAG
                     VMGLELAENFKHRGLDVTLIDQLPQVAFPYDPEIANLVYDKLLKEGLAVHLETRVTEI
                     RDKGREIVLSDGSVLSADMLIFAVGVSPKNEVVKATGIQLSDTGQIIVDDQLQTNLPD
                     IYAIGDIIETTSVVTGQPIQSMLSSAANRQGHMLADILNGTPMRYRGYIGAGVAKIFD
                     HTASYAGMTEHELKASGITNYKTVFITPFDHAYFYPEATRLNLKLIFDADSGRILGGQ
                     AFGEKGVDKRMGELSVAITGNLTVFDLPDLELPYSPPYSTTRDPLNIAGYVAINQMTN
                     IVETIKASDIPENDLKEAFFLDIREPNKAPSGSISATKNIPMNELRDRINEIPKDKKV
                     YITFRRGLNTYTSARILAGLGIKAILIEE"
BASE COUNT         7423 a         4652 c         3985 g         7774 t
ORIGIN      
        1 tgacctgatt attagagaaa caaatggcaa aatagttctt catatttcgc cgtttttcga
       61 gcgaatgccg aggggcagct gattgaacat cgtttatttg gaaaaaggga ttgtgacaag
      121 acgtttgtca caatcccttt ttttatatct tattaattca ttcttttgtt ctattcttct
      181 tcttccgcca tgaatgtatt gaatacttct tcaatcatgt cccattctgc gtctgtttcg
      241 atttgttcta actcgccttc tgttccatct tcgttttcga tgtatgcata agcttgtaat
      301 tctacatctt catcttctga aacaccagca ggatataaaa gaacatagtt acgtccaaat
      361 tcttcttgtc cgtcaactgt tagtaggatt tcatataatg tttcatttcc ttggtcatcc
      421 accaatgtga tgtgttcgtg tccttcatgg tcgtgggaat ggttatgttc tgtcataaga
      481 ttacctcttt tcttttatta ttaaaataat tgcttacctg agccgtctaa ataattttgt
      541 aagatcatca cagctgctaa tttatcgatg actttttttc gtttggctct tgaagtattt
      601 gcctgctcta ctagcatacg ttctgcctga acagtggtta atcgttcatc ttgataatca
      661 actggcaagg agaatagttc tttgatcttt tctccgtatg acatggaagc ttctgccctt
      721 ggtccgatgg aattgttcat atttttaggt aatccaacta caaaccggtc tacttcatat
      781 tgtgctacta attccttcaa ccgatcaaag ccaaactctt cttctgcttc attgatccga
      841 atgatctcta ctccttgagc tgtccagcca aaggggtcac tgatagcaac acctactgtc
      901 ttagaaccga catccaagcc cattactctc ataaattcac tccatgattt gagaggtagt
      961 ccttgacaat tacttccatg atctcatcgc gttcgtgacg gcgaatcaaa tttcgagcat
     1021 cacgataacg aggaatgtag gccgggtctc cagaaagcaa gtagccgaca atctgattga
     1081 ttggattgta gcctttttct tccaaagccc gataaacaat cgctaatgtt tcgctcactt
     1141 cttttttgcg actatcgtca aaaccaaaac gtacggtttc atctgtaaaa cccatgattc
     1201 tacacctctt tcccgcaagg taattccagc ttaccttcta caaccttatt ttactcgaag
     1261 aaagggaaaa ctacaaacaa atattccaaa cttccgaata aattaagaaa cactatttgt
     1321 atgttttttt ccttttttct gtagctttat caaaagtact cttttcataa agtatacgct
     1381 atatttcttt gaatgtatgt ttttacacaa ttttttcata atattcataa aaaattcgaa
     1441 tagctgctac taattttttg tttttgttgc ttgtcctgtc tcataattga tcgtgtagcc
     1501 atcttgaaca ttgtagataa agacattgaa ttcaattttt ttgtcttcaa tgctttctgc
     1561 ttccaattgc actcccctgg ctaccagttc attcccctca aaatgaggag tgacacggta
     1621 gcgtacatga tgatttgttt ctttgatata tgccgcaata tcattttcgt gagcaagcat
     1681 gtatggagca ttcaacgaac gtgtccctgt catcaagttt ttaatgttgt tattttgacc
     1741 agtcagctgg aaaccaatta aatggctgcg attatataac cattgcccgt cacttaattt
     1801 cttttgtttc caacctgtcg ggtcaatata caaaggctct ctcttttcag tagggaacaa
     1861 atcttttcca agcatagcat tggcttgtcc tactctgttc aatttatcaa tatttgaata
     1921 gctttcccat gttcctttgg aaaggctaag atcctctttt gtaaattccg gctgattccc
     1981 attgatttct atttcttgat gttttccatc atattctggc acatccgcta cttgagaagc
     2041 ttgtggtgtg gaatcaaaaa tagttgtaat ctgctgaaag atatctgaat ctttttgtgt
     2101 ggtggaaaca ccgaatgccc ctgcaatcac agcagcgacg atccccacga tcgccttggt
     2161 tgtttttttg ctcatttctt tactccttta ctaacagctc cctaaatctt aacatatgtt
     2221 cccctataaa taaatactag aaagaaatag aaacagcaaa aaataaaacc tgttttaaga
     2281 tggcttaaaa atacaaaaaa gagacggatt cctcgtctct aatcagtcac cactctatct
     2341 gtaaggtatt tattattatt caaaaatttt cttcatactc aagacaatac actgatttca
     2401 caaccgcttg gtaaacggtg cgcaaaagct tgcatctgcg gtaataagtc catccaagtt
     2461 aaaaacataa gaagttgttt ttgcttggct gttcttatta cttgttgcag agcagctttt
     2521 tcacaaccta ttttaagcgg gtgacgggaa tcgaacccgc gacatcagct tgggaagctg
     2581 ggattttacc actaaactac acccgcaata caaaaacagt atacgcctct tttattcctt
     2641 cctcaagttt ttttattctt tacgcttctg gtatgttttg tagtaaaata aaactaatca
     2701 atcaattatt ggagaagatg ctatgacggg aaaagaatta gatctgttgc tggacacttg
     2761 tttactcgct gggaaaatca tgatggaaag caatgcggag atgtatcggg tagaagatac
     2821 gatgagtcga attgctttag cttctggaaa tttccgtttg gtcagctatg tcactcaaac
     2881 cggtctcttt attggtcttg atcgtacctc gaccatccga atggaacaaa ttaccaaccg
     2941 ttcgattaat ttagaaaaag tagtaaaagt aaatgatctt tcacgtaaat atgttgcttc
     3001 agagctaaca ttagaagaac tttactgcga actgcaagca atcgaccgag accgttcttt
     3061 ctttcctgtc tggttgcaaa tcgttagcgc ggcagtgatc agtggaacaa ttatggtgtt
     3121 atttggcgga aagatgaccg atttacctgt gactttagtc attggaggat tgggatacgc
     3181 agcttatcta tatagtttga aattttttag aatcaaattt ttatcggaat ttttatcttc
     3241 tttattgatt ggcatagccg ctatctacag cgtacgtttt ggcttaggaa gcaatcagga
     3301 tctaatcatt atcggatgtg tgatgccttt agttcctgga gttcaaatta cgaacgctat
     3361 ccgtgatttg ctagctggac attatgtctc aggagtttct cgcggaacag aagcgatgat
     3421 gactgctacg atgatcggtt ttgcgattgc ttttattttc cagctttttt attaggaaag
     3481 gaacacaagt atggggacga tatttattca gttttctttt agttttttag cgactgcggc
     3541 atttgccgtt attacaaatg tacctaggcg ttcattgatt tgttgtggtc tttctggtgc
     3601 ttttggctgg atgatctact ggatctgtgt tcaaataggc ggatctgctg cttttggttc
     3661 attgcttggt tctttaggag tagcattcat cagcgatctt ttctctaaac gtttgaaaat
     3721 gcccgtaacg attttcaata ttcccgggat ggttcctcta gtccctggtg gtttagctta
     3781 tcaagcggtt cgaaatctcg ttactgaatc ctatcgagaa gcggctcatt atgcggtcca
     3841 agcaattatg attgctggag caatcgcttt aggtcttgta ttatcagaag tctttaacca
     3901 taacatccgt aatttcaaag taaagcgtga cgcaatcatg atgaaacgaa aaaaacggct
     3961 ttttaaataa acgaagtgta acaaagaaaa ggttgtgaaa gagacgttca gccttgagcg
     4021 atgacaaaat atctcacaaa cattgcgaca agtaaccgca ggagcaataa gaagtaatta
     4081 tgagtattaa aaaaagtttt gtaaaaatag cttctcacat ttttacataa aattgactta
     4141 atactgagaa actgactttt gaacaccctc tatctgtaaa aaaggggctg cgacatgatt
     4201 tttgtcacgg cccctcttct ttattcaaat tttttccaaa acgttcgttg ataataaacg
     4261 atccatagag cggaaagtat tagaactaaa ccgatcacag gaatattcac taaccaaaat
     4321 ggataaaaca aagccaacat tccgtataag acgataagaa taatactgat gatcaatgaa
     4381 cccatcatag ttgcgactaa acccaaactt cctgaacctc tgttaaataa ttggttaata
     4441 ttggtccagt tcagcagcag caaccgataa tctctagcga aataacgtag tgacaataag
     4501 tagcagccga ataaagaacc aaatagcaag ctagggataa aaaacaaagg cagctgaaaa
     4561 gacagtccgc ttaataatgc gatcccacca gttaaaaaac tctgcaatat ccatccgaat
     4621 ctgaactttt cttttaaata ttggttcata gagattggca aagaacggat aaacagaaaa
     4681 ttttcttgat ccaaagaaat aagattgctg ataaaagaag tctgattaac tgttaagaaa
     4741 gccaatgcga tccctaccag gaaaacaaca ccgattaact tattatctaa atcagttaat
     4801 tttatttcgc ctcctagggc aaaagcaaga ataaaaatga ttggagttaa caaggaagaa
     4861 gaaagaactt gcataatcaa attaggttct tttactaatt gagcattgta actaaataaa
     4921 agctgatgca cattttggtt ctttttatgg gttcttttta ttttgaactc actgtttgaa
     4981 tcagtcagtt gctctgtaag ctttggtact atcagctgtt ttacagcaag gaaaaatccc
     5041 agagccgtga tagctagtat tccccaaccc aataaacctt gttgagaaag tggctgactg
     5101 gcagcatcat agaatggcaa taatatcgaa atcggatgcc gatctgttaa ctcattttca
     5161 atagtccccg tttgatgatt catccacatg attccagcaa atgcgatcag catcgagctg
     5221 ccaagcaaga tactagaaac tgtttttcta tgttttttaa acactgctgt tcgagccaaa
     5281 ccaaaaacaa tcaaactagc cagacaaaaa agaataatca aaaatagaga aaacaaaagg
     5341 attgagataa tgatagtaag caaaatccct agatcagctc gccatccggt taaaataaac
     5401 atgactagaa gcggaaaaac aaaaggaatg atggtcaaag caactaccaa aatctttgct
     5461 gaaaaaacct gttcttgtcc aaaaggcaga ggcaaaaata cttgcaggtc atttccttca
     5521 aaaaagatat tataaatgac tgatatactt tgagttaaac ctaacatacc aaaaagtgcc
     5581 acgtaataag taaagaaacc tggcaattta acaaagttca tagcaaacat agcaaatccg
     5641 taaatcagta aaaaaatcag ccctgaaaaa agattttgag acaaaagata gatagttaat
     5701 cgctttccct tttttccttt ctctcttgct ttattcgtga gctgtggatt aacatataaa
     5761 agattaactc gaattaattc taataattgt cgcttataca tacggtccat ctcctgtcgc
     5821 ttgccttcca gccattttca aataaatctt ttctagcgtt tcatcaggtg agccattcaa
     5881 taattcacta actgccccct gataaatcaa ttcacctttc ttcaaaatag ccagtttatc
     5941 gcataactgt tgtgcggtat ccaatacatg tgtagaaaaa atgactgttt ttccttttgc
     6001 ggcatgagcc ttcatcattt ctttcaaatc aaatgctgct tgaggatcaa gtccttgcaa
     6061 aggttcatcc aatacccaaa tatctggatc tggcagcaac gtgccgatca aaatcgtttt
     6121 ttgacgcatt ccatgtgaaa aacttgcgat tgtttcattt tgatgactat acatatcaaa
     6181 aagcgctgtc aattctgcta aacgcttttc ttttttttgt gtgtctaatt cataggcagc
     6241 tgaaatcaag tcccaatatt ctccagccgt caattgtaaa aaaatatctg gggaatcagg
     6301 cacataaccg attttttgtt tgatactcaa tcgattttcc gtcaatttca ttccatctac
     6361 taaaatcgtt cccgatgatg gttcaataat actaactaaa cttttaatcg tagttgattt
     6421 tccggcacca ttatgtccta aaaatccaaa aatttcccct tgtcttatcg agagagacaa
     6481 gtcttttaat gcttgttttt tcccgtaact tttacttaca ttgatgaatt caatcatttc
     6541 tgtactcctt ttgtttgtta ttacttttta ttgtaccctt cgttattggt ggagtaaatg
     6601 tcaaaaagag tgcataacaa aaaaggatag aaacgactag gctgctactt gggttgttct
     6661 cttctacagg aaaacactct tctaacaggt aagatatgag tctttgtttt ctttaaaatg
     6721 attcattttc tatcttttct tgcataaaat tctttagttt atcaatactt ctctttccca
     6781 tctctagttg ttctccattt gcaaatgtta ctatttcgtt tactctgcta atagatgtaa
     6841 tgttttcagt attaatgata taagctttta aatctttaca taaatactca tattcgagtg
     6901 attttttata ttttttaatt gtgccgttta cgagaatctc ttcatctttc aatactaggc
     6961 ctattttatt tttataacct ggcatgctca ttagataaag gatttcatcg taatggatat
     7021 agtgatcgct attaccataa cggacagttg tccacctttt tttcgtagca tgttgaacag
     7081 gatagttttc tttttctttc aataagtaac tttggatgct ttttttattt ttttcataag
     7141 tgaattcttt tatcaaataa ccactaggcg taatctttcg attaataaca gttattgctt
     7201 tattcggatg agacgttaga taaacaattt tacacttttc attcttttct ctaagcattt
     7261 cgcctagttc gataccgcta taataggcgt tcaaatcgat gtctaggaaa aaaatatctg
     7321 aggaatagat ttcattattt ttaatgaatt caacaaattg tataacgttc aatccctgaa
     7381 cgatctcaaa tttccaagga cttaataaaa ttctggattc ttccaaaatt tctactagca
     7441 ttttttcaaa aaccaaacta tcatccaaaa taaatatacg cgttaccata aaaagtacct
     7501 ccactcgata ttttactatc aaaatcattt catacaactt ttcaacatac atgttaggaa
     7561 taaattgtaa ttctcttcac aatcttttcc aacacctttg aaaccgatat caaatacttt
     7621 caggatgctg cttctaaaat aaatatcaaa caaatgaata catcatgcaa aaaaagccag
     7681 acaaccgacg tccttgcgtg tcagctgtct gagctatgtt tatttatttt gttcttttaa
     7741 aaagaacgat acacatactg atttttcgga gcagagactt tctcagcgtt tgtaacaact
     7801 acagatggga tttcacgctt gaatggttga taatagtctg attggatggt tccttctatt
     7861 ttataccatt catcattttt aaccgttact ccttccggca tatgcgtcaa caaaccaaag
     7921 actcctgaat ctgccacaca atggataatg ccgaatcgga acaagaagat atctgtattg
     7981 ctatcgttcg gtgtttggta aacaaatcct tcatagctga tttttttgcc aatgaattcg
     8041 ctagggtaat tataaattag ctccataact tctaaatagt tttcatctgt gatcttgata
     8101 gtttgcttat ccccatactt ttctttcaac tcattcattt ggtctaagta gtctgatttg
     8161 ttgaaataaa tactggtatc cggctttaag tattgtgtct gcatctccgg atcccctact
     8221 gattctttac ttactgggaa attaaatcct ttggcttcta cgatcgtagt gtcgagacta
     8281 actgttggaa acaacgtccc cacgattact ggcaaagcta gcagtacata agcgattcca
     8341 cgttgataag gttttgacaa cccatggtca tgatcgtgct cgtgtgcctt ttcttctgct
     8401 tttccgtttt tgacccacaa atacagctga actaaagcca aaagagcaga taaaatcatt
     8461 gaaagaatag ctaagtagcg ataatggaca ttgatatatt gatcgagttt gccagaaact
     8521 tgcaagtaca tcatcaattc gaaataccca gataaaatca aaaaccgcag catattttcc
     8581 ctcctaaata aataatgtga atccaataac gctaagggca atcagtccga tcagcgataa
     8641 aataaagcgt cctgtaaaat atcgtttcat catcagtaga tttttgatat cgaccattgg
     8701 gccaaagacc aaaaaggcag ctacaggtgc tgtgccgaac agacttaaca aagaagatcc
     8761 tataaacgca tctgcttctg agcaaagaga catcgtaaag gccaataaca gcatgatgat
     8821 aatggctaaa atctttgttg agccgagcgt tagcataatt ctagttggta aataagtctg
     8881 catcgcagct gcgattaatc cgccaatgat caaataacga cctgtatcaa aaaattcgtc
     8941 gattgaatgt gtcaacacat gcccagtttg atggatcagc tgattttttg gttcattctt
     9001 ctcactttgt tcttcttcat ggacatgatg atggtgattt tctgctactt tagacgtgat
     9061 tttttctttc aatacagcgt ctttttgaaa ataggcaagc cagacaccaa tcacaagggc
     9121 aacaaaaaag ctgcctatca cccgccagct tgccatctgc caagaattgc caaatgcgat
     9181 aaaagtggaa aaaatcacga ttggattgat gatcggagca gtcagcataa aagcaaacgc
     9241 tgtgtgaact ggtacgcctt ttttaacaaa ttgatgaacg atcggtacga tgccgcattc
     9301 acatgaagga aagaaaaatc ctaagatcga accagtaaaa atagagaaga tacgattttc
     9361 aggaagccat ctcttcacac gttctggggt caaaaatact tgtaaggcgc ctgagataag
     9421 acagcctaat aatacgaaag gtaatgcttc aataacgatc gataagaaaa tcgtactcat
     9481 ctgcaaaacc gaatttggaa taaaagaaaa catgagtttc ctccttttta tttctagttc
     9541 attcgctttg caaatatacg cttttattca aaaaaaacaa gttgctctgt aaatttttac
     9601 acaactctta ttttttacca aagcacttca attgactttt tctgttattc ccattaaatt
     9661 aaggtctcat acatatcact tctatcataa aagggagaat aaaaatgaaa caaaccacta
     9721 acttgactga agttcaagac atactgcaac agtcatccgt tgccgtcatt tatctttcga
     9781 tgcctaattg cagtgtttgt cacgctgttc gtccacgact agaggaactt ctcacacatt
     9841 atgatattcc ggcacttcat ttggatgcat ttgaaacacc agaagtagct agtaaatttg
     9901 aagtgctgac tgcgccagtt gtcctgattt ttcatcaagg aaaagaagtc tttcggcaag
     9961 cacggttcat cgactttaaa aaaatccgtt atctactaga tgaactgaca gcagaagatg
    10021 attccctttc ttatgaagaa atcttccgaa caaaataatc gttgtccaaa cagaaaaaag
    10081 cgattgaaca agtgaaagtt ttgccttcat ttgttcaatc gctttttatt tttattctgc
    10141 tgtattttcg gaatgcgcat ttgcgtcaga atgcaagaaa tagatcaagg tttgcaattc
    10201 attcgtcaaa tcaacatttt ggatcaatac atctgacgga gcattcaatc gagcagcggt
    10261 aaagttcaag attcctttga ttcctgcttc cgttaactga ttgactactt cttgtgctct
    10321 ttcagcgggt atcgttaaaa ttgctacttc gatttgttgg atcttgattt gttctgacat
    10381 atcttccatt ggataaacgg ggataccgtc tacgatgcgc ccaacgattt cctcattcac
    10441 atcaaaagca caactgatac gtatgctatt actttgatgg aatttgtatt ttagtaaagc
    10501 acttcccaag ttccctacac cgatcagcgc aacatttgtc aattgatcgt cgtttaatgt
    10561 ctttgcaaaa aagtgcataa gatcttctac atcataacca tacccacgtt ttcctaattc
    10621 gccaaaataa gaaaaatctc gacggatcgt tgcactatct acttgaacgg cttcacttaa
    10681 ttcagttgaa gacacttttt tctttccagc attatttaaa atccttaaat agcgataata
    10741 caaaggcagt cttttagctg tcgcctttgg aataatatga tctttcacga aatgacctcc
    10801 acgtattgtc taattacatc tttgatttct ccatcatagc agtatttata ggaaaaaatc
    10861 aattccattg ctcagatatt gtgaaatttt attctttatt catttattta tcatttctgc
    10921 aaatctcgca gtataatgga atttatggta gactactaat tgaaaaatag ttttttattt
    10981 gaggagaatt cacaaagtag aaaagaggat tccatgatat tattacaagc caatcagatt
    11041 gctcgccatt ttggtgcaga agtcttattt gaaaacatac atttagaaat cagtacaggc
    11101 gctcgtgtcg ctttagttgg cagaaatggc gctggaaaat cgactttatt aaagatcatt
    11161 gctggaattg aagcacctga tgaaggaaag atcgctaaaa ataaaacagc gactctcgga
    11221 tacctcgctc aggacacagg acttgattcc aatgaaacag tttggaacga gatgctgaaa
    11281 gcttttgaac aagttagaca aatggaagag cggatgcgcg agctagagat tttcattagc
    11341 gaaggtacgc cagacacacc tgcttatagt tcttatttaa aagaatacga ccggctgcaa
    11401 cacgattttt cagatcgaaa cggctatggt tatgaaaatg aaattcgttc cgtattgcat
    11461 gggtttaaat tcgattcttc attttatgat cagaaaatcg atactttatc aggcggacag
    11521 cgcacacgtt tagctttagc tcgcatgctt ttgcaacacc ctgacatttt gattctagat
    11581 gaaccaacga accatttaga tatcgagaca ctttcctggc ttgagaatta tctgcaaagc
    11641 tatactggtg cattattgat tgtttcccat gaccgttatt ttttagataa agtagtcaat
    11701 gaagtctatg aattaagcag aagaaaaatg cgccattaca aaggtaacta ttcgaaatat
    11761 ctagatttga aagcagaaca gttagcaagt gaatggaagg cttatgaaaa gcagcaaaca
    11821 gaaatccata aattagaaga cttcgttgct cggaaccttg ttcgcgcctc taccacaaaa
    11881 cgagcgcaaa gccgcagaaa gacattagaa aaaatggacc ggctggatcg tcctcaaggg
    11941 gatgaaaaac aagcaaactt tctattcagt atagaaaagc cttctggaaa tgtagtcttg
    12001 caaatcgaag atgccgctat cggatatgat aatgtcacct tatctgaacc aatcaatcta
    12061 gatatccgtc gtcaagaagc gattgctcta gtaggtccga atggtatcgg gaaatcgacg
    12121 ttgctgaaat caatcattca ccagatccct tttatccgtg gaaaagaaac gctagggaca
    12181 aatgtcacca tcggctatta cgatcaaggt caagctgatc tgcatacaaa caagacgatc
    12241 ctagcagaat tgtgggatga gcatcctacc actccagaaa aagagatccg aagtgcttta
    12301 ggtgctttct tattttctgg cgaagatgta gaaaaaccaa tccctctgct aagtggcggt
    12361 gaaaaagcac gggttgcttt agccaagtta gcgatgaaca aagaaaattt tttgattttg
    12421 gatgaaccaa caaaccattt agatattgac aacaaagaag tattagaaaa tgcattgatc
    12481 gattatgacg gcacactgct ttttgtttcc catgaccgat atttcatcaa tcggatcgcg
    12541 acaaaagtcg tcgaactctc tgaaaatggc agcaaagtct atctaggaga ctatgattat
    12601 tatttagaga aaaagcagga agaagaagaa atcgccgaat tgttggcaca agatgagcca
    12661 gaatctgctc ctgctcccac gaacaaatct gattattacc aaagcaaaga acagcaaaag
    12721 cttatccgta gccttcaacg aaaaattact caggttgaag aagaaatggc gcggatcgat
    12781 gaatggatcg atcagttgga acaagaaatg gtcgcgccag aaaacctgga agatcacgta
    12841 aaactcaatg agctgaacca agatttagaa gctgctagac aagatcaaga aacaaaattg
    12901 acggaatggg aagaactaag cttggaatta gaagaatatg aaaattaacg aaaaaaccaa
    12961 aacagaaacc attcatgctg ttttggtttt tcttttttta ttttatcaaa caaaaattcc
    13021 atatttacct ttttcaccaa taagaagacc aattactagt cattctcact aaagcttcta
    13081 aataatagta atcaccccaa atacaaggtg tatttacccc attatttttg ggcttattat
    13141 aaacagactg tgttaaaaag ccatcttctt cttccgttgt agcatatctt tctttcaatt
    13201 gtcgcataat tttccaaaca gcagactggt ataagttctt ttctgttttg tctaatagac
    13261 ctgattctat taattctaat aatccacaaa ccatgatcac catactagat gtatcttttt
    13321 cttctctgct agaatcatca aagatcaaat cccaataaca aatggtatct tctggcaacc
    13381 gattcataaa ataattggct aatattttgc ttaaatcaaa atatttttgt tctttcaaat
    13441 aacgataact taatgcaaaa ccgtaaatcc cccatgcttg gcctctagcc caacaggaat
    13501 tatcactata accttgatgc gtacttccat atcttggttt tccatctttg acatccatat
    13561 aaaatgtatg ataagttgaa ccatcttctc ttactaaata atctgctgca ttttgaatat
    13621 gagcctgagc agctttttta tatttttgat ttccagtttc ttcactcgcc caatacaata
    13681 atggaagatt catgcaacaa tccatgatca ttctaccttg ttgtgaagga tcactaggat
    13741 ctccccaagc ttgtataact cctgccttct cccaaaaacg ctgaagtaat gtttctgcag
    13801 catcaattga agcttccttc atttttcgat ttcccgttat tttataatct gctacggttg
    13861 ataaagaata caaaaaacca agatcgtgat gattagtgat aatttgacag tcaatcctgt
    13921 ggataaaatc tatatctgct ttttcagctg tttgttgaaa taaaggatca cccgtcttct
    13981 caaataacaa ccaattaatt cctgtccaaa atccttctgt ccaaccaata ttgtctctta
    14041 atggataacg tccttttata ggacactcgc ttgggaattg atactgactg ttactgatac
    14101 gtttgattaa cctctccgtg tgtctgcgac aacgctcgat ctcattattc cattcttttt
    14161 ccattaattc tttataacat tcatttatcc atttttctgg atgtattatc tcttcaaaca
    14221 tcactttatc aagcatttcc ccaccagcct ttagcataat tttagtcttt ttttattgtt
    14281 tttattttgg atagttagcc cggttgtccc atcaaaatca attttgggac gattgttcca
    14341 tacttctaaa ctataatccc gatcttgatg agcaaccact cctgttccga gccaatgggt
    14401 tccttttcca aatgtaccct ctaatccagg aataaaagtg ttttcccatg tcatcaagtt
    14461 tgtattgggc aaacccataa tagctgtgga ttttcgttta atagtatcgt cacttaaaca
    14521 gataatacct gaaaagccag cttcactcac cctatagttt tccttgtctt cttctatgat
    14581 tattccttct ttctcaggag aacattcaat agcaaaccct gtctcatata gaacatattc
    14641 tgtatctaat tctactttgt gtacgcgaat ctgccagcct tcaaaaggaa taagccaagt
    14701 tctgattttt gcctgtaaag taggttccca tgtagaggca agtacctctt tcttacatac
    14761 aatgtctcgt acattatttc gaacgatgaa ctgtccatag gttttgatgc tctcattctt
    14821 aaaactggtc gtgatatatg tgtccgctcg ctgaatccct agcattgaat ccattgccat
    14881 ttctgctttc aattgattcg ctcgcggtat cgaaaaacca aattgactag aataagcaaa
    14941 ttttaaatat ttttcattgg tcaatgtatg gaaatttggc cctaattgtc ccgcattcaa
    15001 aaaaaatgta tgtccattat catgttgtat gagcaaattg gcttctgaaa gaagtttagt
    15061 tccttcacga ataggcatag gcatttcttt agccgtccaa tactcatggt cttcaggtag
    15121 cgccaacaat aaaaaaatct tattcagcca taatggagat aacgtactat tatagggttc
    15181 agtcaatgtc aattgagggt aggtataacc aatattcagg ataccttttt catcaaatat
    15241 ttctttttgc atccaccatc ttatattccg attgactaac cccttgattt cacctaattt
    15301 aaaaggcaaa agatcggccc acaacatagc tgaccaaaag cttgaaactg caaaacgata
    15361 aatcatgctt cgaccaaaag gcacatttgc accatcttcc gcaaaaaaat gaatatagtc
    15421 tctagaaaaa tcaatagctc ggttcttata tatctggcta atgggctttt ctggttctag
    15481 tacgctataa atcaaaccat aataatgaaa tgcaaaagga ttataataat cttggcgacc
    15541 tcttgaactg tctcgatacc agccattttg aagataacaa ttttctatat tatcaaatgc
    15601 ttctttcagt gctgattctt taactggata accaaatttt tggataattg ttccgacaat
    15661 tatcttaaaa aattgccaat taccatctgc acattcatat tcaaaaattt gcgagaacca
    15721 atgaataatc tggctttttt ctcctgtatc taattttttc cataataatt gttcatgatg
    15781 atataacgct aataacattg cgggcatttc taccatacgc tggtctcttt catgtatttc
    15841 tccccaataa tcgggatggg aaggatctgt tccagctttt attcctgata gaattaattt
    15901 caaccatcgt tcatctaatt ggtcaattgc gggccccgct ccccataaaa cccgagagaa
    15961 tccttccatt cctgcaattt tatttccata tcctacccct tgatgtatat aatcaatttg
    16021 tgcgccactt gctcgataaa acggctggag tggttgattt atctttttaa ataagtcaaa
    16081 gtaatcctgt ctggtcctta atagtggtac tggatgtttc attgtatttt ctcctctttt
    16141 tactaccaaa tagctaattc cgactgttct aatcgaagta agccttcaat aaaaaaataa
    16201 tcagaataga tcagtggcac attataagtc agttctctat ggtatgcttc tgctccgcct
    16261 ccaatgatcc catcttcatc catattccaa tttgcatagt gcttttctgt cttttggagt
    16321 atattgatag cagcttcatg atacattttt gatcgtacag aggaaacata tcctgctaat
    16381 tccagaagac cgcatgcagc gcatactcca gcactggtat caatattcga atcttttaca
    16441 gcaaaatcaa tgagaggaat ataaccagtt ttttcgattt tctgaataaa atttactgct
    16501 atcttttctg ctatagctag atagtacgga ttttttgtgt aacgataact taacgcgaat
    16561 ccatataaag cccatgcatt tcctctgctc caacaagaat tctcattgta tccttgcccg
    16621 ccaagtaacc gaatatattt ccctgattct gcatcaaatt ctgcgatatg cccggttgat
    16681 ccatctttcc tcactagata cttagctgtt gtatctgcat gctgccgagc aattttagag
    16741 aacctcggat caccagtttc cctcgaagcc caatacagta atggtaaatt catcatacta
    16801 tcaataatca tccaaccagg tttatggtca ttccatgcta ctaaatagtt ccctgaaata
    16861 ttatagcgtc cagcaagtac cgtagctgca tgtaacccta gttgtcgact atgtttgttt
    16921 tgagactgtt gataatgtgc aatagccgat ggtaagaata aaaaacctac atcatgatga
    16981 acgtctgaaa aatggtacag ggcagattct aataattgtt cttgttgtat tgctgcctct
    17041 ttgaactttt ctcgttttgt cttgtgaaag agctgccata aaataccact ccaaaaacca
    17101 ttggtccacc agtcgattcc tctgtccttt attacgtctg tatattttcc gttgtaagaa
    17161 atatatggaa ttttattccc cattctgtct aattccgcat ctatcttttc agaagctaca
    17221 ttcaaaattt ttcttgacca ttccttcgtc tgttcattca tggatatccc gccattcttt
    17281 taatttttat gaatgtggag ttgctcctag aacgaactag atggttaaca ttcgttattt
    17341 tttttactta tttgcagctc caatgtcatt ctccttaaat ttagactgcg cccacacata
    17401 aatcaagacc attgggatca ctgaagttgc taaaattagc ataattgcgc cataattaat
    17461 tggctcacct gtactagaaa ccgtcatatt tttgataatt aagggaaccg tatatttatc
    17521 tttatctgaa agcatgacta atggaaatag atattgattc caacttccca taaagataag
    17581 taaaccacta gtaataatcg taggtgataa aatcggtaca gcgattttcg taaaaatcac
    17641 ccactcattg gcaccatcga ttctggcagc ctccatcagt gctgtaggaa attgactgct
    17701 atattgtcgc atcataaaca ctgaagttgt actggcaatc gaagggatga tcacacctgc
    17761 ataagtattc gataatccta aacgactcat aatgtaaaat aaaggaatca atgtggtaaa
    17821 aaaaggcacc atcctagata gcataactgt attgaacaac agtttattcc ccataaaacg
    17881 atactttgct aatgcataac ccgccattgt agaactggct gtagctaaaa ctgtactgat
    17941 tacagcaata acaatcgaat tccaaataat caagggataa ttatatctcg ccatgatggc
    18001 ttgccagttt tttaatagca tatccccaaa cttgaaattg attccgccac ttaaaatttc
    18061 ttgattactt tgcgttgaag ctacaatcat atataaatat ggcatcaaaa atattaaggc
    18121 accaaacaca ataatgacgt acgtaaaaat tctgctttgt atcttttgtt tagtcatttt
    18181 tatcacctcc gaatttaaat tgaatagccg ataatataac agatacaaac accatcgtcc
    18241 aggcaatagc agaggctttt ccaaaatcaa ccatttcaaa acttgtttta tataaattga
    18301 ctcctacgac caaagctgaa ttttctggtc cattggaagt cccaaagaat atatttggga
    18361 tattgaaatt acccaagcca ttgatagtgg ctaaaactgt ggccattaaa atgattggct
    18421 ttagcatggg gatcgttaat aaaaagaatt gccgaaattt cgatgctcca tctatttcag
    18481 cactttcgta tatctcttta gaaatccctt gaagaccagc taaaaataaa atagtgttat
    18541 atcctacact aagccaaatc atcgtgatga tcattgatat tcgtatccaa aaaggatttg
    18601 ttaaccactg tacaccgtta attcctagcg cattcaaaaa tccattgaat aacccagtcg
    18661 gattgaaaaa agtcatgaat acggaagcta cagccaccgt tgatgtaatg gttggaatat
    18721 aatagacagt tctaaaaaat ccttttcctt ttaactgtgc tgaattcaat aaaacagcaa
    18781 agtaaatagc taatagcatc gttattggaa ttgatcctgc catgataatc aaaacattcg
    18841 tgtaagattt aataaacaat ggatctcgga agatagctag ataattgtca aatccaacaa
    18901 attcaaatga gtagccaatt ttcataaaac taaaagcaaa cgagataatg atgggaatca
    18961 aaatcgtcac aataaagaaa ataacaaaag gtgtgaccaa tccataagca ctttttttat
    19021 gagacgtatc tgttgctttt ttcttgctgt tctttattgt cttgcttggt aacgaagatt
    19081 gcatcatcgt taagtcctcc taatattttt taatcaccag ttaattttac gccgtactta
    19141 tgagcaaaat tttctgcttc ttttagatat gaattttcaa aatcacctgt ttgccaatag
    19201 ttatatgcag cggtagtcaa ataagacgta gcatctgaat aagtggctgg taaaacagta
    19261 tttgatgtat tttcattgat tttattcaat agttcatagt atttttgtcc tccttagtag
    19321 tcaaactttt tctcagctgc ttctgtttga taggccaatg agttagatgt agccacgccc
    19381 agctccagtg cgttggctaa gcattcttca tctgtcagca taaatgtaag aaattgttgt
    19441 gccacttgtg gatttttggc atctttaggg acataaaaac ttgatcctcc tgatactgca
    19501 ccttttcctg gattttcttt tgaaaaagga atcagtggag ccatgcgcca tttgtcagat
    19561 gcatcaggaa aattcaaaga catattcgta gcataccagc cgccagcaac aaacattgca
    19621 gattcttgaa atgctttctc agcatcttgt gcaccgtaga aatgtacaat atctgagtcg
    19681 atcatttctt tgattaactt tgcagcatgt ttcgcttctt tagtattcag acggatattt
    19741 ccttcttcat caagcaaaga ttgattctgc tgggctagca tgttgataaa caattcttgg
    19801 tccccagatt ctggcaaggc aattccaaaa ctaccagttt tttcttttaa tgcttttgcc
    19861 acctggatat attcttccca agaactgatt tcttcaaaat ctacacctat ttcagcaaat
    19921 ttttttggtt gataataaca aagtgctggg gtaaaatcac tagcccaagg aataatcgtt
    19981 tcattactcg tttgttgacg caaaatattc aatttttgat cataaaactg atcgccatat
    20041 ttgtcataaa atccatattc atcagcacta taaaaagcat cttgaaattt ttccagcaca
    20101 cctgctgctg aagtgtctgc catggagata atatccggca tatcttcgtg tgttaccaat
    20161 tttggggtga tcatttgtac tgttgctgtt cccgaagcta cattcacaat ttttaagtca
    20221 atatttgctc cagttttgcg gttatagatt tttgcagcat tttcaacata gtttgtcagt
    20281 ttgccgtttg cgacatatac ttctaatgta gtggcttttt ttatcattgg atctgcgatg
    20341 tttgctgtta cttcttcgtt agtttttcca catgcactta agctgaataa tagggtaatg
    20401 tttagcaaag ctatcaccaa tttgtatctt tttttcatgt ctatcccttc tttcttttca
    20461 agttcttata taaaacgctt tcacttatgt atattaacgg aattagtaac acaaatattt
    20521 atataaaaaa atattttact tgtcgttttg gcttttcatt tttgttataa tagtcattaa
    20581 ttataaatag aaagcttttt atataaaaag agaaaggaaa gtggttcttt tgttaacaaa
    20641 caaccaatcc tccaatcttg tcaaaaaaac actaacctat tccatagata cccatcttca
    20701 tttatctgct gctccctatt ttcgactact aataaatgaa agcgctaatt tatatgctga
    20761 atttgcacat aaatctatag aactagataa tccttccctt ttattcattg ataaacagca
    20821 agaagtccaa atcttttcta ctcaaaagga aacacaaact tttatcatta tccagtttta
    20881 ttttgatgag caattattag cacatattac gggacaatct ttttctatag atcaagaaat
    20941 aacatctatc attacttttc tgaaaaatga acagttcaaa attgattatc taaaacatag
    21001 taagccagct atttcctatg atcaggaaat caattctttt ttcatactat gccaacagat
    21061 gagcattcat ttgctttcct tattagttac ttattgtctg caaaaaattc aattgtcttc
    21121 cttaccaagg cattatcaac aggaacttgc tttattgaca ataccttcaa atgagaaagt
    21181 cattcctaaa aaaaagcata ttctttattc agatgaatta gttgatccaa tcatacgata
    21241 tttagaagaa catctttcag aaaatttttt gcttgaacaa ttagcccaaa ttttttttat
    21301 cagtccagcc agcttaaaaa aacattttag aaaagtgaat ggaaaaagtg ttattagcta
    21361 ttttaaagaa ctaaaaatgg aaaaagcaaa acgtctcgtg ctagaaaatg aattaaccta
    21421 tacagaaatt gcagatatat taggatttca ttcgatccat catttttctt cagcttttaa
    21481 aaaatataca gggttatccc cttcaaagta ttatctatcc ttaaaataga aaaaaatttt
    21541 taggttttat ccacaaatta tttctctata ttttctgtct attcaaaata aacaataatt
    21601 ttccggcaca attatttgct gttttttacc ataacatagt acgatcaacg tagaaaagga
    21661 gatgttttta tgaaagttgt tgtcattggc ggtgtagctg gcggtccttc atttgccact
    21721 cgtttccgtc gattgaatga agcgcacgaa atcattatct atgaacgcgg agaaaatatt
    21781 tcttatgcaa gttgtgcttt gccttattat ttaggaggtg tgatcactga ccgtgactcg
    21841 ctgatcgaac gtacaccaga aatattgaaa acaaaaaaca acatcgatgt ctttactaaa
    21901 cacgaagtaa cagcaatcga tccttctact aagcgattaa cagttaaaga cctatccaca
    21961 aatgaagaaa taaaaacaga ttacgataag ttgatcatct cttctggtgc tagaccagat
    22021 tatccggata ttcccggagt ttttgaagca gaaaacggct ttgtcctccg tagtgtgacg
    22081 gatgcggatc gaatcaaatc gttccttgaa gaaaaaaatc cacaacatgt cgtcattctt
    22141 ggtgcaggtg ttatgggtct ggaattagct gagaatttca agcatcgcgg cttagacgtg
    22201 actttaatcg atcaattgcc acaagtcgct ttcccttatg atccagaaat tgctaattta
    22261 gtttatgaca aattgctgaa agaaggatta gccgttcatt tagaaacaag agttactgag
    22321 atccgtgata aaggtcgaga aatagtatta tcagatggct ccgtcctttc tgctgatatg
    22381 ctaatttttg ctgttggtgt ttctccgaag aatgaagtgg tgaaagcaac cggcatacaa
    22441 ttatcagata caggtcagat cattgttgat gaccagttac aaaccaatct gccggacatc
    22501 tatgcgattg gcgatattat cgaaacgact agtgtagtga ctggtcagcc gatccaaagt
    22561 atgctttcca gtgcggccaa tcgtcaagga catatgctgg cagatatttt aaatggtacg
    22621 cctatgcgct atcgcggata tattggtgca ggtgtcgcaa aaatctttga tcatacagca
    22681 agttatgcgg gaatgacaga acatgaatta aaagcatcag gcataacaaa ttataaaact
    22741 gtttttatca ctccctttga ccatgcctat ttctatccag aagctacaag attaaatcta
    22801 aagctgattt ttgatgcaga tagcggtcgt attttaggtg gacaagcatt tggagaaaaa
    22861 ggtgtcgata aacggatggg agaactttct gtagcaatca ccggaaactt gacagtcttt
    22921 gatttgcctg atttggagtt gccttactct ccaccgtatt ctactacccg tgacccgttg
    22981 aatatagctg gttatgtcgc aatcaatcaa atgacgaata tcgtagaaac gatcaaagca
    23041 agtgatatac ccgaaaacga tttgaaagaa gcgttctttt tagatatacg tgaacctaat
    23101 aaagcacctt ctggcagtat ctcggcaaca aaaaatattc caatgaatga gttacgagat
    23161 cgaatcaatg aaatcccaaa agataaaaaa gtttacatta ctttcagaag aggattgaat
    23221 acttatactt ctgcccgaat cttggcaggt ttgggtatca aagcgatttt gattgaagaa
    23281 taaaaaaacg accgcggtgg gtagccgcgg tctaggagtt atttttactt gaggagtaaa
    23341 aatgaaaaag tatatagggt tgttggttgg tatgtatata gaatagaaga taaatgtgaa
    23401 gaaattgtgc attatttctt cacatttatt aggttatagt ttaagaaagt ttaaacattg
    23461 cgcaatttaa ttattttagc tgtttctttt cataaaaaaa catgttgtga ctctgagaca
    23521 aatcatagtc catagtgatg tctctagagt aactacgcct aatattacga ggcatcatat
    23581 taatctatca gcactagtac ctaaactagc atccctttcg aatatttttt cttatataat
    23641 aaagtttgct ttcactcctc taatctcacc aaatcgactg gcttgcgttg tgtgatacta
    23701 ggaactgatt ggtttagaac gacttttcta ttgtaatcat atttttcgcc atcctcgttg
    23761 tcttctaatc cttcaatgaa tgatccctaa tgtgtgtatt aaataattta atttttttgt
    23821 tcctatttat ttaa
//
LOCUS       DACEEZ010000038        23648 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20313_2017
            SAMN12639966-rid9879223.denovo.038, whole genome shotgun sequence.
ACCESSION   DACEEZ010000038 DACEEZ010000000
VERSION     DACEEZ010000038.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639966
            Sequence Read Archive: SRR10023158
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 23648)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23648)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741683.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741683.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:44:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,016
            CDSs (total)                      :: 2,962
            Genes (coding)                    :: 2,889
            CDSs (with protein)               :: 2,889
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 36 of 73
            Pseudo Genes (incomplete)         :: 29 of 73
            Pseudo Genes (internal stop)      :: 18 of 73
            Pseudo Genes (multiple problems)  :: 8 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23648
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639966-rid9879223.denovo.038"
                     /isolate="E20313_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(55..789)
                     /locus_tag="IWO89_001525"
     CDS             complement(55..789)
                     /locus_tag="IWO89_001525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aquaporin family protein"
                     /protein_id="HAQ0456273.1"
                     /translation="MDYSLMTRLAAEMIGTALLIVLGNGAVANVDLKGTKGYGSDWML
                     IAVGYGFGVMMPAMIFGGISGNHINPAFTIALASNGLFPWAEVVPYIIAQLIGAAIGQ
                     LIVVACYKPYYDQTEDVNHVLGTFSTINSVGSKFNGFVNEFFGSFVLFFGALGITKAP
                     FFQDNLGTAHLALGFLVWTLVASLGGPTGPGLNPARDFAPRLVHALLPLKHKGSSQWG
                     YAWVPVVAPICAGVVAVFLYKLLFMA"
     gene            1116..1526
                     /locus_tag="IWO89_001526"
     CDS             1116..1526
                     /locus_tag="IWO89_001526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CidA/LrgA family protein"
                     /protein_id="HAQ0456274.1"
                     /translation="MKFFQEFFIIIFFSVLGEGIRIFAHLPIPGSILGILFLFLAFEM
                     HLVDPEKIGATGDFLLNNLAILFVPAGVGLLEYFDDIASIWPVLLGAVVVCSIVTMVA
                     AGKTAEGVEALLGYVRKKKAERVSVNEMEENQID"
     gene            1519..2220
                     /locus_tag="IWO89_001527"
     CDS             1519..2220
                     /locus_tag="IWO89_001527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LrgB family protein"
                     /protein_id="HAQ0456275.1"
                     /translation="MTEFFVNPLFGLSLTLGVYVLSDKFLRKYKLPVLNPLVLSIVVI
                     ILFLHFSGISYKDYNIGGNFLTMLITPATVALAIPLYKNFRLLKENFYPVMAAILAGI
                     VANCLVSVGFGYFFSLHKEMVISLLPKSVTTAISVDLSHTMGGVNAVTLAIVVSTGIF
                     GSLIATHIFRLFKIESPVARGVALGSTSHAIGTAKAIEIGEIEGIISGLAICVNGILT
                     VLLLPLFFQPFAGLF"
     gene            2220..3806
                     /locus_tag="IWO89_001528"
     CDS             2220..3806
                     /locus_tag="IWO89_001528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002358389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M protein trans-acting positive regulator"
                     /protein_id="HAQ0456276.1"
                     /translation="MRKPLFYATITKTFEHKKGGKDVYLSSEYLKQFDKDLYYKILLL
                     ESFEDQAWHTAAQLAQVVQLDARSVSKYLNELSKNYQQFSGKTHPLFTKNHRSGYNFY
                     DTLDSIEHERFLIYLVQSTLKFQLLHDIFFEEFHTMYQFAQKHYISESTAHRKINEWK
                     QQLQTYGIRLQRGTYIAQGEEEIIRLYLHMTFWQLFRGKIWPFETISQMDVKNMAEHI
                     MAFFNVRLNEIKKRRLEYMLGAFFLRKSQKHYVVLNEKKRRLISDNLLFQRFCQVMEP
                     VFPNYFQVEDELGALFLVLMTREEYYSDPKIRKKIFDFHQQAKTPPFTALSEAKAALS
                     LYQEEQGLPAENLTFEAENYLFSSHFFAYLFPNAKETIDGNSSDFINHLVIENKELKQ
                     WLVHFFESRHKHPNHLAFKNHAFLMGRYLTVFKTLGAFTPQLPKITILLMTDFPLFEE
                     QLLEEGLRTFFRNEYQLIFLPTDYRGREVDLLISTSKVHRKPWADLDYFIVTEELKLI
                     DYIQLSQKFEMIQKQKLLKQ"
     gene            3825..4046
                     /locus_tag="IWO89_001529"
     CDS             3825..4046
                     /locus_tag="IWO89_001529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016186310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2829 domain-containing protein"
                     /protein_id="HAQ0456277.1"
                     /translation="MTFEEILPELKKGAKIIRKGWSGFELYVVLVSDDEFDGCPVTPY
                     LLIKTSDEGFSSFAPTVCDILADDWAIVE"
     gene            4043..4804
                     /locus_tag="IWO89_001530"
     CDS             4043..4804
                     /locus_tag="IWO89_001530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP reductase"
                     /protein_id="HAQ0456278.1"
                     /translation="MKNQYPDLAKQVVFITGAASGIGAAQARAFLEQDAFVFGLDQKF
                     GKMEEISLRFPETFAYALGDVRQMKELQQAVSKCQNHFGEVTILLNTAGILDAYKPLM
                     ETDESLWDLIYETNVKSMYQLTKIILPDMIRQKSGTIINMASIAGLIAGGGGIAYTSA
                     KHAIVGFTKQLALDYAGQGIRVKGIAPGAIQTPMNAADFEGKGEMAEWVANETPVKRW
                     AQPEEVAELTLFLASPQASYIQGTIIPIDGGWLLK"
     gene            5184..5693
                     /locus_tag="IWO89_001531"
     CDS             5184..5693
                     /locus_tag="IWO89_001531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="QueT transporter family protein"
                     /protein_id="HAQ0456279.1"
                     /translation="MEKNNTRSAKRWTTTDVTRMALVTGLYVAVTLVLSVFSFGVIQI
                     RLSEMFNYLSLYNKRYIVAVTAGVALANIASPLGLIDVAVGSVSTLLVLLINYKITSR
                     IKNMKIKMAVTACVFAFSMFTVAGQLTILYQLPFFLNWWVIALGELASMIVGGIIIYW
                     IGKKVDLSK"
     gene            complement(5768..6307)
                     /locus_tag="IWO89_001532"
     CDS             complement(5768..6307)
                     /locus_tag="IWO89_001532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin transporter BioY"
                     /protein_id="HAQ0456280.1"
                     /translation="MNRTLKELILAAEFAAIISVLSQMTIPFGLVPLTGQTFAIGLTA
                     TFLGRKLGLISVGVYLLLGLIGLPVFSGMTGGIAVLFGPTGGYLIGFLLQTWITGWMI
                     ERTDAHYLYAIFANLMGSLAALICGTIWLKISGDLTFTTAFASGLLPFLLPEAIKATG
                     AAYIGILMKKRLNRFLHTN"
     gene            complement(6447..7058)
                     /gene="rpsD"
                     /locus_tag="IWO89_001533"
     CDS             complement(6447..7058)
                     /gene="rpsD"
                     /locus_tag="IWO89_001533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010766410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HAQ0456281.1"
                     /translation="MSRYTGPSWKISRRLGISLSGTGKELARRPYAPGQHGPNSRGKQ
                     SEYGMQLTEKQKLRHMYGMNERQFRNLFVKASKIKEGKHGVNFMILLEQRLDNVVYRL
                     GLATTRRQARQLVNHGHILVDGKRVDIPSYHVEVGQVISVREKSQNIVTVKEAVEATV
                     GRPAFVSFDAEKLEGSLTRLPERDELYPEIDEALVVEYYNQSL"
     gene            7374..8171
                     /locus_tag="IWO89_001534"
     CDS             7374..8171
                     /locus_tag="IWO89_001534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate-nitrite transporter"
                     /protein_id="HAQ0456282.1"
                     /translation="MNPVSPLFEKIDASIEKKANLFENSFSRYAVRAVLACLFLTLGT
                     AVAFAIAMKGEDIAHGLGKILYAFMFSWGLVMILYMNAELGTSNMLYMTVGVFRQRLA
                     LSKAAKILFACIFFNMIGGILCGFLISMTTPFQDLTLDNFMLESIAVKLAKPSATILV
                     EGMFANIVVNTAVLISMRMKEDVGKVITVVFIIFIFAFLGYEHVIANFPAFTLTYFAS
                     HGQMDGMTVSNVLHNLFFALAGNYIGGGLVMGLGYAWLNQSKSSYVD"
     gene            8199..8834
                     /locus_tag="IWO89_001535"
     CDS             8199..8834
                     /locus_tag="IWO89_001535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA alkylation repair protein"
                     /protein_id="HAQ0456283.1"
                     /translation="MEVLVFPKNEEKALQMEKYMKNLFSFAGVPKPERALLQQPLLDE
                     SKNLSISEVLAFVHYYYEQIEREYQYVAIDLVQKNIKRLTFEDLVVLLPLVQKKEWWD
                     SIDSWRKVYSTWCKAYPEQREEVFQLFEKKEDFWLRRIAITLQLGYKQQTDTGLLIRA
                     IDEDRQTKEFFIQKAIGWALRDYSKTDADWVKRQLTTDLSKLAVREASKYL"
     gene            8854..9627
                     /locus_tag="IWO89_001536"
     CDS             8854..9627
                     /locus_tag="IWO89_001536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HAQ0456284.1"
                     /translation="MRRKLFAFDIDGTLLDSNKQALDSTREALEKLRKAGHLVTIATG
                     RSRFHAQEIIRDLDFTNYILCNGAAGFLDHEQVYKNLLDRDQLDAFVQEAHHEQIDTA
                     FVSLDNIKRSSSNDVTVMEEAMHSFGAVLPELDQFFAEEQEIYQALAFFDQTYDGKFS
                     SYDKIRFVRWHENSVDVVPKNGSKAATILHLAEQVGIPHEDIISFGDGQNDREMLRMS
                     GIGIAMGNAVPEVQAEAKMVTDTNDQDGIWKALKELSVI"
     gene            10035..10832
                     /locus_tag="IWO89_001537"
     CDS             10035..10832
                     /locus_tag="IWO89_001537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003128088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00282 family metallophosphoesterase"
                     /protein_id="HAQ0456285.1"
                     /translation="MRVLFIGDVVGSMGREMITEYLPRLKKKYRPQVTIVNGENAASG
                     RGITEKIYKKFLQDGVDVVTMGNHTWDNRGIFEFVNEAKKMVRPANFPEGTPGQGMVF
                     VKVNQVELAVINMQARSFMVDLDDPFRKMNELVEEARKRTPIIFVDFHGETTSEKQAM
                     GWFLDGKVSAVVGTHTHVQTNDARILPRGTAYLTDVGMTGPYDGILGMRREPVIEKFL
                     TALPKRFEVVEQGRSILSGCILELDDTTGHAKEIQLIQINDDRPFME"
     gene            10810..11223
                     /locus_tag="IWO89_001538"
     CDS             10810..11223
                     /locus_tag="IWO89_001538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0456286.1"
                     /translation="MIDRSWSRKRGSFLQQEPEIQKEVRKLTKLLRENETIIRYKELE
                     EKIQQNQYLAELREKIKQAQKDAVHFAHYDKPAAEKEAIKQADQFMQEFDQHPLVVAY
                     RKQLLEADDLLHHLTTMIQEEINGQIEEEKHASKN"
     gene            11207..13852
                     /gene="mutS"
                     /locus_tag="IWO89_001539"
     CDS             11207..13852
                     /gene="mutS"
                     /locus_tag="IWO89_001539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HAQ0456287.1"
                     /translation="MPQKTKNTPMMEQYLSIKAQYQDAFLFYRLGDFYELFYEDAIKA
                     SQLLELTLTSRNRNAEEPIPMCGVPHHAAQGYIDALIEKGYKVAICEQVEDPKTTKGM
                     VKREVIQLVTPGTVMDSKGLSAKDNNFLTAVVSQGNEFGFAYADLSTGELKTARLHDE
                     EAVLNEASALQTKELVLGSEITDTLREQLSGRLGLVFSQQNEMEENAEFSFLTSELVD
                     PLEKEATGKLLTYLSVTQKRSLAHIQKAVEYQPDHFLKMDYYSKFNLELSQSIRTGKK
                     HGTLLWLLDETKTAMGGRLLKQWLDRPLIQAKQIKARQEMVASLLDSYFERIDLQSAL
                     TKVYDLERLAGRVAFGNVNGRDLIQLKTSLEQVPMIRGLIEGIDQGQWQSLLSEMQPM
                     DHLVQLIDQAIQDDPPLQITEGNIIKDGFNEQLDMYRSAMRNGKKWLAELEAKERQET
                     GIKNLKIGFNRVFGYYIEITKANLGNAELEKYERKQTLANAERFITPELKELETQILE
                     AEEKSVDLEYQLFLAVREEVKKAIQPLQVLAKAISAVDVLQSFATISERYQYVRPELV
                     SDKHQLLIKDGRHPVVEKVLGHQEYIPNSVEMAEDEMILLITGPNMSGKSTYMRQLAL
                     TVLMAQMGCFVPAKQAVLPIFDRIFTRIGASDDLIAGQSTFMVEMMEANQALRYATPN
                     SLILFDELGRGTATYDGMALAQAIIEYIHRHVQAKTLFSTHYHELTVLEKELPQLKNV
                     HVGAVEKDGEVVFLHKMMDGPADKSYGIHVAKIAGLPSNLLERAADILHTLENGDNGH
                     HTVPAEVEEKTKTAKQEQIKPPAEKVNQDHIQDRVKEENQQLSLFSELSENETEVIES
                     LKQINLLEMTPMDALNTLYELQKQL"
     gene            13870..15978
                     /gene="mutL"
                     /locus_tag="IWO89_001540"
     CDS             13870..15978
                     /gene="mutL"
                     /locus_tag="IWO89_001540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutL"
                     /protein_id="HAQ0456288.1"
                     /translation="MAKIQELSERLANQIAAGEVVERPASVVKELVENAIDAGSTQID
                     ILVEEAGLRTIQVIDNGEGILADDVENAFKRHATSKIHNRDDLFRIRTLGFRGEALPS
                     IASVSEMTLETANQEEKQGTYLSLKGGEVIEHRPAPLRQGTKITVSNLFFNTPARLKY
                     VKTLHTELANIGDIVNRLALSHPSIAFRLVHDGNKMLATAGNGDLKQTIAGIYGLSTA
                     KKMLKIEGKDLDFELFGYVSLPEVTRASRNYLSTIINGRFIKNFSLNKAIVAGYGSKL
                     MVGRFPIAVLEIKMDPLLVDVNVHPTKQEVRLSKEKELTELISNAIQKALSQENLIPS
                     AADNLRFKKKVPDQPKTEQIEIDLSYAFEEEKPKKPSSLNFDRETGTFFVEELKNTFV
                     DRIVDKSVENVENYSGFSEENTTYPEKTAKTVDKFGENVDNFVEKTVEKSEKKEFPIV
                     DNMEKTNEEQADKSVEEDEKIYEEELTLHPEFDFTQGNDKTLEQAIKKLETEKPVQRF
                     PQLEYFGQMHGTYLFAQSSDGLYIIDQHAAQERIKYEYFREKIGEVSDDLQELLVPFV
                     LDYPNSDALKLKEQKHLLEDAGIYLEEFGQNSFIVRAHPTWYPNGEEESIIREMIDML
                     LTTGSVSVKKFREATAIMMSCKRSIKANHYLNEAQARVLLKDLARCENPFNCPHGRPV
                     LIHFTNSDMERMFKRIQDPH"
     gene            16000..16560
                     /locus_tag="IWO89_001541"
     CDS             16000..16560
                     /locus_tag="IWO89_001541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum formation inhibitor Maf"
                     /protein_id="HAQ0456289.1"
                     /translation="MKIVLASQSPRRKELLAHIVSEFEIEPADIDETPFPDEQPVDYV
                     RRMAEEKARTVWEQDEKEDHLVIASDTTVVLNQEIMGKPEDLAEAEFMLKKLSGETHT
                     VYTAVVLKTKEREERILAEAHVTFYPLTDEEIKRYLETGDYADKAGAYGIQNAASVFV
                     KEISGDYYSIVGFPIGAVNQALKTFI"
     gene            complement(16645..18633)
                     /gene="treP"
                     /locus_tag="IWO89_001542"
     CDS             complement(16645..18633)
                     /gene="treP"
                     /locus_tag="IWO89_001542"
                     /EC_number="2.7.1.201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system trehalose-specific EIIBC component"
                     /protein_id="HAQ0456290.1"
                     /translation="MAKYQADAEKLLHDIGGKENIAAVSHCATRMRFVLNDPGKADEK
                     AIEDIPSVKGMFTNAGQFQVIIGNDVSTFYNDFVAVSGVEGVSKEQGKAAAKQNLHPV
                     QRAIAVLAEIFTPLIPAIIVGGLILGFRNVLEGIQFESLGGTIVEHSKFWNGVNAFLW
                     LPGEAIFHFLPVGITWSIAKKMGTTQILGIVLGITLVSPQLLNAYSVASTAAADIPFW
                     DFGFAQVQMIGYQAQVIPAMLAGFMLAYLEIFFRKYIPQSISMIFVPLFSLLPTVLAA
                     HVILGPIGWTVGSWISNIVNTGLTSSISWLFSAVFGFLYAPLVITGLHHMTNAIDMQL
                     IADFGSTNLWPMIALSNIAQGSAVLAIIFLHRGNKKEEQISIPAMISCYLGVTEPAMF
                     GINLKYVYPFVAAMVGSGLAGMFANLMGVRANAIGVGGLPGILAIQAETWVPFIIAMI
                     IAVIIPFGLTIIFRRQGILNKIDPAVPENAADVQLQTANGATATPQSFEPVSATGTAV
                     ATKETLFAVAAGTIKEITEVNDPVFSQKMMGDGYAVEPSNGKVYAPVNGKVTSVFETK
                     HAIGILSNEGLEVLVHMGLDTVELKGVPFNVFVKEGYLVTPETLIAEMDLPEIEQAGK
                     KTDIIVALTNNEKVAGLSLDQSGLVRPGEAVGKAEVKS"
     gene            18856..19569
                     /gene="treR"
                     /locus_tag="IWO89_001543"
     CDS             18856..19569
                     /gene="treR"
                     /locus_tag="IWO89_001543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trehalose operon repressor"
                     /protein_id="HAQ0456291.1"
                     /translation="MNKFNEIFLDLEQKILAKEYPPHSLLPSENQLIKIYGVSRETIR
                     KALNLLTTEGYIQKKQGKGSIVLDRNRFDFPISGLTSYKELQETQRIPSETIIHTLEE
                     TEVTKALNEITGWEIGAPVWHLIRERKINGEVVILDTDYLLKEIVPILTPAQAQGSIY
                     EYFENELSLTIAYAQKEITVEEVTEIMKTAMDLHDDTYAVVVRSLVHLEDTRCFEYSE
                     SVHRLDKFRFVEFARRRKV"
     gene            19646..20092
                     /gene="msrB"
                     /locus_tag="IWO89_001544"
     CDS             19646..20092
                     /gene="msrB"
                     /locus_tag="IWO89_001544"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase MsrB"
                     /protein_id="HAQ0456292.1"
                     /translation="MDKPIRPEKEELKEKLSPMAYAVTQENATERPFSGKYDDFYEKG
                     IYVDIVSGEPLFSSAEKYDAGCGWPSFSKPITKRQVREKADFSHGMHRIEVRSKEADS
                     HLGHVFTDGPVDQGGLRYCINSASLKFIPYEKMDELGYGEFKSLVE"
     gene            20262..20864
                     /gene="ruvA"
                     /locus_tag="IWO89_001545"
     CDS             20262..20864
                     /gene="ruvA"
                     /locus_tag="IWO89_001545"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HAQ0456293.1"
                     /translation="MYEYLTGVVTFINPYYIVVETNGIGYQIALGNPYRYSSKLNQKI
                     KIYVHQVIREDAHLLYGFDSLEEKQLFLRLVSVSGIGPKSALAIMASEDHSGLIQAVE
                     SSDVTYLTKFPGVGKKTAQQMILDLKGKFGELSIDTPFSLFDEANTKDATALSEAIEA
                     LSALGYSDREIKRVEKQLKEVDNQTTDEYLRQALKLMMKK"
     gene            20877..21878
                     /gene="ruvB"
                     /locus_tag="IWO89_001546"
     CDS             20877..21878
                     /gene="ruvB"
                     /locus_tag="IWO89_001546"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HAQ0456294.1"
                     /translation="MTDEYLLSPESGENELSLEKSLRPQLLSQYIGQRKVKQELQIYI
                     EAARNREEPLDHVLLYGPPGLGKTTMAMVIANEMTVNIRTTSGPAIERPGDLVAILNE
                     LEAGDVLFIDEIHRLPRVVEEMLYSAMEDFYVDIMVGQGPTAHPVHFPLPPFTLIGAT
                     TRAGMLSAPLRDRFGIISHMEYYDEQDLQEIVLRSSDIFQTEIIEEGALEIARRSRGT
                     PRIANRLLKRVRDFAQVQGDGKIDRPIADQALTLLQVDQAGLDYVDQKLLRTMIELYG
                     GGPVGLSTLSVNIGEERETVEDMYEPYLIQKGFLKRTPRGRIATAYAYEHFGYDYHI"
     gene            21907..22485
                     /locus_tag="IWO89_001547"
     CDS             21907..22485
                     /locus_tag="IWO89_001547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family transcriptional regulator"
                     /protein_id="HAQ0456295.1"
                     /translation="MSDTTRTKQLIKNVLIDLCQTQSFRKISVQKIANEAKINRQTFY
                     YHFTDKYDLLRWVYCEDALHYLDPQCLSLDNWEEQALKMLKAIAEKKEFYANTVLSDP
                     EILQKEFSQLIQKSFIKIFEQMDEERQLSARDKLFYARFFSYGCSGVLVNWITNEYEE
                     TPMEIAMQLFRLAKDTELYSYRLYAKEELEEQ"
     gene            22537..23019
                     /locus_tag="IWO89_001548"
     CDS             22537..23019
                     /locus_tag="IWO89_001548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low molecular weight phosphotyrosine protein
                     phosphatase"
                     /protein_id="HAQ0456296.1"
                     /translation="MTKILFVCLGNICRSPMAEGLLRKRIALEGREAEFFVDSAATST
                     YEVGKTPHPGTKKILNQEKVDMTNMVARQITPHDFETFDWIIGMDQENVEELKRRAPR
                     SAQGKIHLFLSSIPGKEKENVPDPYYTNNFDETYHLITEGLNHWWNIWIDENKITNNL
                     "
     gene            23136..23510
                     /locus_tag="IWO89_001549"
     CDS             23136..23510
                     /locus_tag="IWO89_001549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0456297.1"
                     /translation="MNEETVSPMKKLTKLVTHTSYQLFDSFVNDTEEHAKNVRLQGKF
                     ILELQKAATEHSLVILQVRANRLSSKYETISGWVATKTVEKDRVMIRLQNEEQQIRMV
                     PVEQIEKITILSPAGEQERLSK"
BASE COUNT         7728 a         4272 c         5066 g         6582 t
ORIGIN      
        1 ataaaaaaac ttggtacgca atcgctgtac caagtttttt tatttagctt ttgattaagc
       61 cataaataat aatttatata agaatactgc tacgacacca gcacagatag gtgctacaac
      121 tggtacccat gcatatcccc attgagaaga acctttatgt ttcaatggaa gaagtgcgtg
      181 tacaagacgt ggagcaaagt cgcgggctgg gtttagtcca ggacctgtag gaccaccaag
      241 tgaagcaact aatgtccata caaggaaacc aagtgctaag tgagctgtac caagattatc
      301 ttggaagaat ggtgcttttg taatacctag ggcaccaaag aataaaacga atgaaccaaa
      361 gaattcattg acgaaaccat tgaatttaga accaacgctg ttgattgttg agaatgtacc
      421 taacacatgg tttacgtctt ctgtttggtc gtagtaaggt ttgtaacaag ctactacgat
      481 caattgtcca atcgctgcac caatcaattg tgcaatgata taaggaacaa cttcagccca
      541 agggaataga ccattagatg ctaaagcaat tgtaaatgct gggttgatgt ggttaccgct
      601 gattccacca aaaatcattg ctggcatcat aaccccgaat ccatacccga cagcaatcag
      661 catccagtca ctgccatatc cttttgtacc ttttaaatct acgtttgcta cagcaccgtt
      721 acctagaacg attagtaatg ctgtaccaat catttccgcc gcaagacggg tcattagtga
      781 gtaatccata aatacctcca aaagttataa aaatgcaaaa aaaaaagaaa catcaacacg
      841 aaaagcatcg gtgcagtgtt tcgtgtcgga aggatttctg cagtcttaac aaatgtgaat
      901 aaaaaaatga attccattac ttataagaaa ccagaccaac agtaaaactc caactctttt
      961 taaaagttct ccacaaatca ccaagcttaa ctataaatat accaaaaaaa aaacgttttt
     1021 tttctcaaaa attgtatctt agaaacagca tgttttgaaa ctttcagaaa aggtttgtta
     1081 gtaaaatgaa agctatacgt aaggagtgtt gtttgatgaa attttttcaa gagtttttca
     1141 ttattatttt cttttctgta ttaggagaag gcatacggat ttttgctcat ttacctattc
     1201 ctggaagtat cttaggtatt ctttttttat ttttagcatt tgaaatgcat cttgttgatc
     1261 ctgaaaaaat cggagcaact ggtgattttt tattgaataa tctagccatt ttatttgttc
     1321 ctgctggtgt aggtttattg gaatattttg atgatattgc ttcgatatgg cctgttttac
     1381 tgggagcagt cgtcgtgtgt tccatcgtca cgatggttgc agcgggaaaa acggcagaag
     1441 gtgtagaagc tttgctgggc tatgtccgta agaaaaaagc agaaagggta tctgtaaacg
     1501 aaatggagga gaatcaaatt gactgagttt tttgtgaatc cattatttgg cttaagtttg
     1561 actttaggag tgtacgtatt atctgacaaa tttttgcgga aatataaact gcctgttttg
     1621 aatcctctcg tattatcgat tgttgttatt atcttatttt tgcatttttc tgggatcagc
     1681 tataaagact ataatatcgg gggcaatttt ttgactatgc tgattactcc tgcgacagtg
     1741 gcacttgcca tccctcttta taaaaacttc cgtttactga aagagaattt ttatcctgta
     1801 atggctgcga ttttagcagg aatagtcgca aattgcttgg ttagtgtagg gttcggttac
     1861 ttcttctctc ttcataaaga gatggtgatc tcattactac ctaaatctgt aacgacggct
     1921 atttctgtcg atttatctca tacgatgggc ggggtgaatg ccgtgacact tgctattgtt
     1981 gtctctacag gtatttttgg ttcgctgatc gccactcata ttttccgcct attcaagatc
     2041 gaaagtccag ttgctcgcgg tgtggcgtta ggaagtactt ctcatgctat tggtacagca
     2101 aaagcaatcg agattggtga aatagaagga atcatttccg gacttgcgat atgcgtcaac
     2161 ggaatattga ccgttttatt gctaccgtta ttctttcagc cttttgctgg cttgttttga
     2221 tgcgaaaacc actattttat gctacaataa ccaaaacgtt cgagcataaa aaaggaggga
     2281 aagacgtgta tttgtcttcc gagtatctta aacaatttga taaagatcta tattataaga
     2341 ttctcctcct agaaagtttt gaggatcaag cttggcatac cgctgcacaa ttagcgcaag
     2401 ttgttcagct ggatgcccgc agcgtgtcga aatacctgaa cgagctgtct aaaaactatc
     2461 agcagtttag cggtaagaca caccctttgt ttacaaaaaa tcaccgttct ggttataact
     2521 tttatgatac gctcgattca attgaacatg agcgtttttt gatctatctc gtgcaaagta
     2581 cgctgaaatt ccagttgctg cacgacattt tttttgaaga attccataca atgtaccaat
     2641 ttgcgcaaaa gcattacatt agtgaatcta ctgctcatcg aaaaatcaat gaatggaaac
     2701 agcaactgca aacttacgga attcggctgc aacgaggtac atatatcgct caaggcgaag
     2761 aagaaatcat tcgactttac ttgcatatga cattctggca gttgtttcga gggaaaatct
     2821 ggccatttga aacgatttct caaatggacg taaagaatat ggcagaacat atcatggctt
     2881 tttttaatgt tcgtctgaac gagatcaaaa aaagacgatt ggaatatatg ctaggtgcct
     2941 tttttttaag aaaatcacag aagcattatg ttgtattgaa tgagaaaaaa agacggctga
     3001 tttctgacaa tctgttgttc cagcgatttt gccaggtcat ggagccggta tttcctaatt
     3061 attttcaagt agaagacgag ttaggcgcat tatttcttgt actgatgact cgtgaagaat
     3121 attatagtga tcctaagata cggaagaaaa tttttgattt tcatcaacaa gcaaaaacac
     3181 cgccctttac agccctttca gaagcaaaag ccgctctttc tctttatcag gaagagcagg
     3241 ggctgccagc agaaaatcta accttcgagg cagaaaacta tttattcagc agtcattttt
     3301 tcgcctatct ttttcctaat gcgaaagaaa cgattgatgg caatagtagt gattttatta
     3361 atcatttagt tattgaaaac aaggaattga aacaatggct cgtacatttt tttgaaagta
     3421 gacacaaaca tcccaatcat ctggcattta aaaatcatgc gtttttgatg gggcggtatc
     3481 tgactgtgtt taaaacatta ggagccttta ctccacagct tccaaagatc acgatccttt
     3541 taatgacaga tttcccatta tttgaagagc aactgttaga ggagggatta cggacatttt
     3601 tccgtaatga gtatcagctg atttttctgc caacagatta tcgggggaga gaagttgatc
     3661 ttctgatttc gacaagtaaa gtccaccgca aaccatgggc ggatcttgac tattttattg
     3721 tgactgaaga actaaaatta attgattaca tccaactatc tcaaaaattt gaaatgattc
     3781 aaaaacaaaa actattaaaa caataacaaa gaaggagaaa aaagatgaca tttgaagaaa
     3841 ttttaccaga attgaaaaaa ggtgcaaaga ttattcgtaa aggatggagt ggatttgaac
     3901 tttatgttgt tctcgtatcc gatgatgaat ttgatggctg tccggtgacg ccgtacttat
     3961 tgatcaaaac aagtgacgaa ggtttttcca gcttcgctcc tacagtttgt gatattttag
     4021 cagatgattg ggcgatcgta gaatgaaaaa ccagtatcca gatctagcta aacaggttgt
     4081 atttatcaca ggagctgctt ctggaattgg tgctgcgcaa gcccgagcgt ttcttgaaca
     4141 agatgctttt gtatttggat tagatcaaaa gttcggtaaa atggaagaaa tttctttgcg
     4201 atttcctgaa acttttgcct acgcactagg tgatgttcga caaatgaaag aactccaaca
     4261 agcggtatcg aaatgccaaa atcattttgg tgaagttacg attttactga atacagcggg
     4321 gatattggat gcctacaaac ccttgatgga aactgacgaa tcgctgtggg atctgattta
     4381 tgagacgaat gtgaaaagca tgtatcagct gacaaaaatc atccttcctg atatgatccg
     4441 ccaaaaaagc ggaacgatca tcaatatggc ttcgattgct ggcttgattg ctggtggagg
     4501 tgggatcgct tatacaagcg cgaaacatgc gattgtcgga tttaccaaac aactcgctct
     4561 ggattacgca ggacagggta tccgagtaaa aggaattgct ccaggagcca tccaaacacc
     4621 aatgaatgcg gctgattttg aagggaaagg tgagatggca gagtgggtgg caaatgaaac
     4681 accagtcaag cgatgggcac agccggaaga agtagctgag ctgacgttat ttcttgcctc
     4741 tcctcaagca agctacatac aaggaacgat catcccaatc gacggcggat ggttgttaaa
     4801 ataaccttgt gtttttttca agcttctgta tgataaaact aaagagagaa acaacggttg
     4861 atgataaaaa aagtcgatga ctgttcccga cattcgatgc cgaacagcca gttcaaaaaa
     4921 ttctttttaa taatcgttaa tattcgaaac taagcggctt ttcacgacct cttgatataa
     4981 acgatattca aaagcttgca actgcggtaa caagtccagc caaatgaaaa atataaggag
     5041 ctatttttat ttgaccattc ttattacttg ttgcagggca gctttttcac taccttctgc
     5101 gacacttcag ttggaatttt cttcccatcc tttggtcgcg gtcattacca agataaaatt
     5161 atcttgcaaa ggagaaatag aacatggaaa aaaataatac ccgttcagcc aaacgctgga
     5221 caacgacaga tgtgactcgg atggcgctag tcactggttt atatgtggca gtgacgctgg
     5281 ttttgtctgt ctttagcttt ggagtgattc agattcgtct ctcggagatg ttcaactatc
     5341 tgtccttgta taacaaaaga tacatcgtcg cggtaacagc gggagtagct ttggctaata
     5401 ttgcttctcc tcttggactg atagatgttg cagtaggaag cgtcagcacg ctgcttgtat
     5461 tgcttatcaa ctataaaatc actagtcgca tcaaaaacat gaaaatcaaa atggccgtga
     5521 cagcatgcgt atttgctttt tccatgttta cagtagctgg tcagctaacg attttatatc
     5581 aactaccttt tttcttgaat tggtgggtga ttgctttagg agaattggca tcaatgattg
     5641 tcggtgggat aattatttat tggatcggta aaaaagtgga tttgtcaaaa taaagatctg
     5701 tttaatttaa aatagggcag catggaagag actgaatgtt gagtcttctt ccatgctgct
     5761 tttttgatta atttgtatga agaaatcgat ttagccgttt tttcatcaag ataccgatat
     5821 aagctgctcc agttgccttg attgcttccg gcagtaaaaa cggcagtagt ccactggcaa
     5881 atgcagttgt aaaagtaaga tcaccactta ttttcagcca gatcgttccg cagatcaatg
     5941 cagcaagaga gcccattaga ttggcaaaga ttgcatataa gtaatgagca tccgttcttt
     6001 cgatcatcca accagttatc cacgtttgta agagaaatcc aatcaaatat ccgccagttg
     6061 gcccaaataa aacagctata cctccagtca tccctgaaaa tacaggaaga ccaatcaagc
     6121 caagtaaaag ataaactcca acagatatca atcctagttt tcttccgaga aaagttgctg
     6181 ttaatccaat agcaaatgtt tgccctgtca aaggaaccaa accaaaagga atggtcatct
     6241 gagaaaggac agaaataatc gcggcaaatt ccgcagcaag tatcaattct ttcaatgttc
     6301 ggttcataac gccctctttt cgttaactta atatttttat ttggttaacg aaattgtaaa
     6361 acaaagtgat tactttgtaa atagaaaata taaaaaaaga ccagctatcc aatagatagc
     6421 ggtctttttc aagaaagctt ttgaaattat aagctttggt tgtagtattc aacgacaaga
     6481 gcttcgtcga tttctggata tagttcgtca cgttctggta aacgagtcaa tgaaccttcc
     6541 aatttttcag cgtcaaagct tacgaatgct gggcgtccaa cagttgcttc tacagcttct
     6601 tttactgtta cgatgttttg tgatttttca cgaactgaga tcacttgacc cacttctacg
     6661 tggtatgaag ggatatcaac gcgtttgcca tcaactaaaa tgtgaccatg gttaactaat
     6721 tgacgtgctt gacgacgagt cgttgcaagt cctaaacggt aaacaacatt gtctaaacgt
     6781 tgttctagta agatcatgaa gttaacacca tgtttacctt ctttgatttt gctagctttt
     6841 acgaacaagt tacggaattg acgttcgttc attccgtaca tgtgacgaag tttttgtttt
     6901 tcagttaatt gcatgccgta ttctgattgt ttgccacggc tattaggtcc atgttgacct
     6961 ggtgcgtaag ggcgacgtgc taattctttt cctgtgcctg acaatgagat gcctaagcga
     7021 cgagaaattt tccatgatgg tcctgtataa cgagacatta aaatttcctc caatgaatat
     7081 aagtttttgg agtaaaataa tccgttgaaa aattcataat catatagttt gttcttcaat
     7141 cttcaccttt gcagccgcgg ttacacaatc gaacccgaga cggggcaaca aactagggat
     7201 gagttattat ttttctgctg cattatttta cacaaattac agtatatgat agatcatcca
     7261 cgaatgtcaa gataattagt acataaaaac gctttacaaa cctaggctgt ctttataaaa
     7321 tgagagggta tgtgcaaatg gaaaaaagcg aaggaagttt aaggagaaaa tcaatgaatc
     7381 cggtatcacc attatttgag aaaatcgatg cttcaattga aaagaaggcg aatctttttg
     7441 aaaacagctt ttcacgttac gctgttcgag cggttctcgc ctgtttgttt ttgactttag
     7501 gaacggctgt ggcatttgcg atcgcgatga agggggaaga tatcgcgcat ggtctaggaa
     7561 agattttata cgcatttatg ttcagctggg gtctggtcat gattctatac atgaacgccg
     7621 aattaggaac atcgaatatg ctttatatga ccgtgggtgt ttttcggcag agacttgctt
     7681 taagtaaggc agccaagatt ctatttgcct gtattttttt caatatgatt ggcggtatat
     7741 tatgtggatt tctaatttct atgactacgc catttcaaga tttaacgttg gacaacttta
     7801 tgttagaatc aatagcagta aaactagcaa aaccttcagc aacgattttg gtggaaggga
     7861 tgtttgcgaa tatcgttgtc aataccgctg tactgatcag catgcggatg aaagaggatg
     7921 tcggaaaagt gatcacagtc gtattcatca ttttcatttt tgctttttta ggatatgaac
     7981 acgtgatagc gaattttcct gcctttacgc ttacttactt tgcttctcac ggacagatgg
     8041 atggaatgac agtcagcaat gtgttacata atttgttttt tgctttggca ggaaattata
     8101 ttggtggcgg tttagtaatg ggactgggat atgcttggct gaatcagtca aaatcatcct
     8161 atgtggacta aaaagtcgtt cattaaaggg gaagtgccat ggaagtttta gtttttccaa
     8221 aaaatgagga aaaagcgtta caaatggaaa agtacatgaa aaatcttttt tcctttgctg
     8281 gagtacctaa gccggaacgc gctcttttac aacaaccgtt actggatgag agcaaaaatt
     8341 tatctatttc tgaagtttta gcttttgttc attattatta tgaacaaatc gaaagagagt
     8401 atcagtatgt agcgatcgat ttagtccaga aaaacatcaa acggcttaca tttgaagact
     8461 tagtcgtatt gttgccacta gttcagaaaa aagaatggtg ggacagcatc gactcttgga
     8521 gaaaagtata cagcacatgg tgtaaagcct acccagagca gcgtgaagaa gtctttcaat
     8581 tgtttgaaaa aaaggaagat ttttggctac gtcgaatagc aatcacgttg caattaggtt
     8641 ataaacagca aacagacaca ggactattga tccgagcaat agacgaagat cgtcaaacaa
     8701 aggaattctt tatccaaaaa gcaattggtt gggcattacg cgattatagt aagaccgatg
     8761 ccgattgggt aaaaagacag ctgacaacag atttgagtaa gttagctgtt cgagaggcaa
     8821 gtaagtattt ataagaaagc gagacgacaa gagatgagac gcaaattatt tgcttttgat
     8881 attgatggaa cgttgttaga ttcaaacaaa caagctttag atagcacaag agaagcattg
     8941 gaaaaattac gcaaagccgg tcatctggtc acgattgcta cagggcggag ccgatttcat
     9001 gcgcaagaga tcattcgcga tcttgatttt acgaactaca ttttatgtaa tggagcggcc
     9061 ggttttttag accatgaaca agtatataag aatttactag atcgcgacca gttggatgcg
     9121 ttcgttcaag aagcgcatca tgagcagatc gatacagctt ttgtcagctt agacaatatc
     9181 aagcgttctt cttccaatga tgtaacggtg atggaagaag cgatgcattc tttcggtgct
     9241 gtcttgcctg aattagatca gttttttgca gaggaacaag aaatatacca agcgttagca
     9301 tttttcgatc aaacatatga tgggaagttc tcttcatatg ataaaattcg atttgtccgc
     9361 tggcatgaga acagtgtaga tgtcgtacct aaaaatggtt ctaaagcagc gacaattctg
     9421 cacttagcag aacaagtagg catccctcat gaagacatca tcagctttgg cgatggacaa
     9481 aacgaccgtg aaatgttgcg catgtcagga attgggatag ctatggggaa tgctgtgcca
     9541 gaagtacaag cagaagctaa gatggtcact gatacaaacg atcaggatgg aatttggaag
     9601 gcattgaaag agttaagcgt gatttaaaag aggttgtgaa aaagctgttc tgcatcaagt
     9661 aataagccca gccaaacaaa aatagctcct cctatttttc gtttttctgg gcttattacc
     9721 gcagatgcaa gctttcgaac accgtttaag gaagaggtcg tgacagaagt ggtcagtttc
     9781 aagaaataag aagggattta cgaaaattgc ttctcaaatt tttgtaaaat tacggcttat
     9841 ttctgaagaa gtagatgaaa caacgtttat tcgggaaatg ggactgtgac aagatgtttg
     9901 tcatagtccc atttttttat tttatttttc aaaactaaga aataccagga aagagttcct
     9961 tttgtttgac cttttctttc ttattgaaaa caactataat atagagtgat gttaaaagag
    10021 aagaggaatt tagtgtgcgg gtattgttta ttggtgatgt agtaggttca atgggacgag
    10081 agatgattac tgaatatctt cctagattga agaagaagta ccgtccgcaa gtaacgattg
    10141 taaatggtga gaatgccgct tctggacgtg gaatcacaga aaaaatctat aaaaaatttt
    10201 tgcaagacgg cgtagatgtc gtgacgatgg ggaatcatac gtgggacaac cgcggaatct
    10261 ttgagtttgt aaatgaagct aaaaagatgg ttcgtccggc aaattttccg gaagggacgc
    10321 ctggacaagg gatggtcttt gtcaaagtga accaagtcga actggcagtg atcaacatgc
    10381 aggctcgttc ttttatggtg gacttggatg atccttttcg taaaatgaat gagctggtag
    10441 aagaagccag aaaacgtacg ccgatcattt ttgtagattt tcatggagaa acaacaagtg
    10501 aaaaacaagc aatgggctgg tttttagatg ggaaagtttc agcagtggtc ggtacacata
    10561 cacacgttca gacaaatgat gcgcgaattt tgccaagagg aacagcgtat ctgacagacg
    10621 ttgggatgac aggtccctat gatggtattt tgggtatgcg cagagaacct gtaatcgaaa
    10681 agtttctgac tgcattacca aaacgttttg aagttgtgga acaaggccga tcgattttaa
    10741 gcggctgtat cttggaattg gatgatacga caggtcatgc aaaagaaatc cagctgattc
    10801 agatcaacga tgatcgaccg ttcatggagt agaaagagag gcagtttttt gcagcaggag
    10861 ccggaaattc aaaaagaagt aaggaaactg acaaaacttt taagagagaa tgaaacgatc
    10921 attcgctaca aagaacttga agaaaagatc cagcaaaacc agtatttagc tgagttgaga
    10981 gaaaaaatca aacaagcgca aaaagatgct gttcattttg cccactatga caaaccagca
    11041 gcagaaaaag aagcaatcaa acaagcggat caattcatgc aagaatttga tcagcatccg
    11101 cttgttgtag cttatagaaa acagctgttg gaagcagatg atctgcttca tcatttaaca
    11161 actatgatac aagaagaaat caatggacaa atagaggagg aaaaacatgc ctcaaaaaac
    11221 taaaaatact cctatgatgg aacaatatct aagcatcaag gcccaatacc aagatgcttt
    11281 tttgttctat cgtttaggag acttttatga actattttac gaagatgcga tcaaagcgtc
    11341 acagcttttg gaattaacgt tgacgagcag aaatcgaaat gcagaagaac ctattcctat
    11401 gtgcggtgta cctcaccatg cagcacaagg atatatcgat gccttgattg aaaaaggcta
    11461 taaagtagcg atttgcgagc aggtagaaga tccaaagacg actaaaggga tggtcaagcg
    11521 cgaagtgatc cagctagtca ctcctggtac agtcatggac agcaaaggat tgtcggcaaa
    11581 agacaacaat tttttaacag ctgtagttag tcaaggaaat gaatttggct ttgcttatgc
    11641 cgatttaagt acgggagaat tgaagacggc tcggttgcat gatgaagaag ctgttttaaa
    11701 tgaagcctct gcattgcaga caaaagaact cgtattaggc agtgagatca ctgataccct
    11761 tcgtgaacag ctaagcgggc gtttagggct agtcttttct caacaaaatg agatggaaga
    11821 aaacgcggaa tttagctttt tgacaagcga actagtcgat ccgctggaaa aagaagcaac
    11881 cggcaaacta ttgacttatt tatcggtgac acaaaagcgg agtttagctc atatccaaaa
    11941 agcagtagaa taccaaccag atcatttttt gaaaatggat tattattcaa aattcaacct
    12001 agaattaagc caatctattc ggactgggaa aaagcacggc actcttctat ggttgttaga
    12061 tgaaacaaaa acagcaatgg gtggccgttt actaaaacaa tggctagacc gtccgcttat
    12121 ccaggcaaaa caaattaaag caagacaaga aatggtcgct tctttgctag acagctattt
    12181 tgaacgtatc gatttgcagt cagcattaac gaaagtctat gatctggaac gcctagcagg
    12241 tcgagtagct ttcggtaatg tcaatgggcg agacttgatc cagctgaaaa cttctttaga
    12301 acaagtgcca atgatcagag ggttgattga aggaatcgat caaggtcaat ggcaaagtct
    12361 gctttcagaa atgcagccga tggaccatct cgtccaattg atcgaccaag ctatccaaga
    12421 tgatccgccg ttacaaataa cggaaggaaa catcatcaaa gatggattca atgagcagct
    12481 cgatatgtac cgttctgcta tgagaaacgg aaagaaatgg ttggcagaac ttgaagcaaa
    12541 agagcgtcaa gaaacaggaa tcaagaactt aaaaatcgga tttaaccgcg ttttcggtta
    12601 ttatatagaa attacaaaag caaatcttgg aaatgcagaa ctggagaagt atgaacgaaa
    12661 acaaacttta gcaaatgcag agcgtttcat cacaccagaa ctaaaagaat tggagacaca
    12721 aatcctcgaa gcagaagaaa aatctgttga tttagaatat cagttgtttt tagctgtgag
    12781 agaagaagtc aaaaaggcta tccagccgct acaagtactt gcaaaagcaa tcagtgcagt
    12841 agatgtgcta caaagttttg caacgatcag cgaacgctat cagtatgttc ggccagaact
    12901 tgtcagtgac aagcatcagc ttctgatcaa agacggacga catcctgtag tagaaaaagt
    12961 attaggacat caggaatata tccctaacag cgtagaaatg gcagaagacg aaatgatctt
    13021 gctgattaca ggaccgaata tgtctggtaa aagtacgtat atgcgacagt tggcattaac
    13081 tgttctgatg gcgcaaatgg gctgtttcgt tccggcaaaa caagcagttt tacctatttt
    13141 tgatcggatt tttacccgaa ttggtgcaag tgacgactta attgctggac aaagtacttt
    13201 tatggtagag atgatggaag ccaatcaagc cctgcgttat gctacgccaa acagtttgat
    13261 tttgtttgac gaactagggc gaggaacagc aacctatgac ggaatggcgt tggcccaagc
    13321 aatcatcgaa tatatccatc gtcatgtaca agcaaaaaca ctcttttcta ctcattatca
    13381 tgaactgacg gtacttgaga aagaacttcc gcagcttaaa aatgtccatg ttggagccgt
    13441 tgaaaaagac ggagaagttg tcttcttgca taaaatgatg gatggacctg cggataaaag
    13501 ctacggcata catgtggcaa agatcgcagg attgccttcc aatctactag aacgagcagc
    13561 agatatcctg catacgctag aaaatggcga taacggacac catactgttc cagctgaagt
    13621 agaagagaaa actaaaacag ccaaacagga gcaaataaaa ccacctgcag aaaaagtaaa
    13681 tcaagaccat atacaagacc gtgtaaaaga agaaaaccaa cagctttctt tattcagtga
    13741 attatccgaa aatgaaacag aagtcattga atcactgaag caaataaatt tactagaaat
    13801 gacgccaatg gatgcactaa acacattata tgaattacaa aaacaactgt aggaagaagg
    13861 cggatatcga tggccaaaat ccaagaatta tctgaacgtt tagctaatca gatcgctgcg
    13921 ggagaagtag tggagcgacc agcttccgtt gtgaaagaat tagtggaaaa tgcaattgac
    13981 gccggcagta ctcagattga tatcttagtt gaagaagcgg gactgcggac gatccaagtg
    14041 attgataacg gagaaggcat tttggcagac gacgtagaga atgccttcaa gcggcatgcg
    14101 acaagtaaga tccataatcg cgacgatctt tttcgcatac gaacgctggg ctttcgaggg
    14161 gaagctttgc caagtattgc ctctgtttct gaaatgactc tggaaacggc gaatcaggaa
    14221 gaaaaacaag gcacctatct gtcgctgaaa ggcggagaag ttattgagca tcgaccagca
    14281 ccactgcgtc aaggaacgaa gattaccgtc agcaatctgt ttttcaatac gccagcccgc
    14341 ttgaaatacg tgaaaacact gcacacagaa ttagcgaaca tcggggacat cgtcaatcga
    14401 ctggcattga gtcatccatc gattgctttt cgtttagtac atgatggaaa caaaatgctg
    14461 gcaactgctg gaaatggcga tctgaaacaa acaattgcgg gaatctacgg attatctacc
    14521 gcaaaaaaaa tgctgaaaat cgaaggaaaa gatctagatt ttgagttatt tggctatgtt
    14581 tcattgcctg aggtgactcg tgccagtagg aattacttat cgacgatcat caatggacgt
    14641 tttatcaaga acttttcctt gaataaagca atcgttgcag gttatgggtc aaaattaatg
    14701 gtcggtcgtt tccctattgc tgtattggaa atcaaaatgg acccgctttt agtagatgta
    14761 aatgttcatc cgacgaagca ggaagtccgg ctgtcgaagg aaaaagaact aacagagcta
    14821 attagtaacg ccattcaaaa agcattgtct caagaaaatc tgattccaag tgcagcggat
    14881 aatctacggt ttaagaaaaa agtacctgat cagcctaaaa cagaacaaat cgaaatcgat
    14941 ttgtcctacg cttttgaaga agaaaaacct aaaaagccaa gtagtttgaa ttttgatcga
    15001 gagacaggta cctttttcgt agaagaacta aagaacacat ttgttgacag aattgttgat
    15061 aagtctgtgg aaaatgttga aaactattct ggtttttctg aagaaaacac cacttatcca
    15121 gaaaaaacag cgaaaacggt ggataagttt ggtgaaaatg ttgataactt tgtagaaaaa
    15181 acagtggaaa aatctgaaaa gaaagaattt cccattgtgg ataacatgga gaaaacaaat
    15241 gaagaacaag cagataaatc cgtagaagag gatgaaaaga tttacgagga agaattgaca
    15301 ctccatcctg aatttgattt cactcaagga aacgacaaaa cattagaaca agccatcaag
    15361 aaattagaaa cggaaaaacc agtacaacgt tttcctcagt tggaatattt tggacagatg
    15421 cacggaacgt atctttttgc ccaaagtagc gatgggttgt acatcattga tcagcatgct
    15481 gcccaagagc gcattaagta tgaatatttc cgggaaaaaa ttggggaagt atctgatgat
    15541 ctgcaagaat tgcttgttcc atttgtgtta gactatccaa acagtgatgc tttgaaatta
    15601 aaagagcaaa aacacttgct agaagatgca gggatttatt tagaagaatt tggtcaaaac
    15661 agctttattg tacgggccca tcctacttgg tatcccaatg gagaagaaga atcgattatt
    15721 cgggagatga tcgatatgct gctgacaact ggaagtgtga gtgtaaaaaa attccgtgaa
    15781 gcaacagcaa tcatgatgag ttgcaaacga tcgattaaag ccaaccatta tttgaatgaa
    15841 gcacaagcac gtgtgctgct gaaggatctg gcacgatgcg aaaatccttt caattgtccc
    15901 catggccgtc cagtattgat tcactttacc aattcggata tggaaaggat gttcaaacga
    15961 atccaagacc cgcattaagt caaagagaag agggaaaaca tgaagattgt attagcatcc
    16021 caatcaccaa gaagaaaaga gttattggct catatcgttt cagaattcga gattgaacct
    16081 gctgatatcg atgaaacgcc atttccagac gaacagcccg tcgattatgt tcgtagaatg
    16141 gctgaagaaa aagcacgaac tgtctgggaa caagatgaaa aagaagacca tttagtgatt
    16201 gccagtgaca cgactgttgt tctcaatcaa gagatcatgg gtaaaccaga agatctggct
    16261 gaggctgaat tcatgctgaa aaaactaagc ggggaaacac atactgtata tacagctgtt
    16321 gttttaaaaa caaaggaacg agaagaacgg atactagcag aagcacatgt cactttttat
    16381 cctttgacag acgaagagat caaacgctac ttggagacgg gggattatgc ggataaggca
    16441 ggggcatacg gcatccaaaa tgctgcgagt gtattcgtca aagaaatttc tggagattat
    16501 tacagtatcg taggatttcc gattggtgct gtcaatcagg cactgaaaac attcatttaa
    16561 acaaaaaagc ggactatctg attttcgatt gaaaatccat gatagcccgt tttttttatt
    16621 gaaaagatta cttatctact ctgactaaga ttttacttca gctttaccga ctgcttcgcc
    16681 cgggcgaaca agtcctgatt ggtcaagaga aagtcctgca actttttcat tattggtcaa
    16741 agcaacaata atgtcagttt tcttgccagc ttgttctatt tctggcaaat ccatttcagc
    16801 aatcagggtc tctggtgtga caaggtatcc ttctttcacg aatacattga aaggaacacc
    16861 ctttagttca acagtatcta atcccatgtg aacaagtact tccagcccct cattagaaag
    16921 aataccaatg gcatgttttg tttcaaagac actcgtcact ttaccgttta ctggtgcata
    16981 gactttgccg tttgacggtt cgacagcata accgtcaccc atcatttttt gtgagaaaac
    17041 tgggtcattt acttcagtta tttccttgat ggtccctgca gcgactgcga ataatgtttc
    17101 ttttgttgca actgccgtac cagttgctga aacaggttcg aaagactgtg gcgttgctgt
    17161 tgcgccattt gctgtttgaa gttgtacatc agcagcattt tcaggaacag caggatcaat
    17221 cttattcaaa attccctgac gtctaaagat gatcgtcaag ccaaatggaa tgatgacagc
    17281 aatgatcatt gcgatgatga aaggtaccca tgtttctgct tggatagcta gtattcctgg
    17341 caaaccgcct actccgatcg cgttagctcg aacacccatc aagttagcaa acatgccagc
    17401 taatccagag ccgaccattg cagcgacgaa aggatagacg tatttcaaat tgattccgaa
    17461 catagcaggt tcagttacgc ctaagtaaca agaaatcatt gctggaatcg agatttgttc
    17521 ttctttttta ttcccacgat gtaagaagat gatggctaag actgcagagc cttgggcgat
    17581 atttgataaa gcaatcatcg gccataaatt cgttgatccg aaatctgcaa tcagctgcat
    17641 atcgatggca ttggtcatat ggtggagacc agtaatcact aaaggagcat acaggaatcc
    17701 gaatacggca ctaaacaacc agctgataga agacgtcaat ccagtattta cgatatttga
    17761 gatccagctt ccgactgtcc agccgatagg acctaaaata acgtgtgcag ctaaaacagt
    17821 tggtaacaat gaaaacaatg gaacaaaaat catcgagatc gactgaggga tatatttgcg
    17881 gaagaagatt tctagatacg ctaacataaa ccctgccagc atggctggaa taacttgcgc
    17941 ttgataacca atcatctgga cttgagcaaa accaaaatcc caaaatggaa tatctgctgc
    18001 ggctgtggaa gctacactat aagcatttaa taattgcgga gaaactaacg taattcctaa
    18061 aacgattcct aatatttgtg tagtacccat cttttttgca atactccaag tgatacctac
    18121 tggaagaaaa tggaaaattg cttcacccgg taaccacaaa aaggcattga caccattcca
    18181 aaacttcgag tgttcaacga ttgttccacc aagactttca aactggatac cttcaagtac
    18241 atttctgaat ccaaggatca atccaccgac aatgattgca ggaatcagtg gtgtaaagat
    18301 ttctgctaaa acggcaatag cacgttgaac gggatgaagg ttttgttttg cagctgcttt
    18361 tccttgttct ttggaaaccc cttcaactcc agatactgca acgaagtcgt tataaaaagt
    18421 agaaacatcg tttcctatga tgacttggaa ctgtcctgca ttcgtgaaca tcccttttac
    18481 gctaggtata tcttcgatcg ctttttcgtc cgcttttcct ggatcattca ggacaaaacg
    18541 catacgagtc gcacaatgac taactgcagc aatattttct tttccgccga tatcatgtaa
    18601 cagcttctcg gcatctgctt gatattttgc catatcaata aacccctttc tcttaacttg
    18661 tctatgcaag ttgtttcttc atttactata ctttttttcg gagagaattg caagcgtttt
    18721 attaaaaagt acgagagaaa atgatatcgt tttaaaaagc ttaaaataag cttttcttaa
    18781 aaataggaac attgaaatac aaaaagagct gtagtatagt gaagaaatga tggaagaacg
    18841 agtgagggaa aaaacatgaa taagttcaat gaaatttttt tagatttgga acagaaaatt
    18901 ttagcgaaag aatatcctcc gcattcgctt ttacccagtg agaaccaatt gatcaaaatt
    18961 tatggtgttt cacgagaaac aattagaaaa gcgctgaatc ttttgactac ggaaggttat
    19021 attcaaaaaa aacaaggaaa aggttcgatc gtactggatc ggaatcgctt cgactttcct
    19081 atttctggat tgactagcta taaagagcta caggagaccc aacgtattcc aagtgaaacg
    19141 atcatccata cgttagaaga aacagaagtg acaaaagcat tgaacgagat cactggctgg
    19201 gagatcggtg cgcctgtttg gcatttgata cgcgaaagaa aaatcaatgg cgaagtagtt
    19261 atattagaca ccgattatct attaaaggaa atcgttccta ttttaactcc agcacaagca
    19321 caaggatcga tctatgaata ttttgaaaat gagctttctt tgacgattgc ttacgcgcaa
    19381 aaggagatca cagtagaaga agtaacggaa atcatgaaaa cagcgatgga tcttcacgat
    19441 gacacttatg cagtagtagt cagaagcctc gttcatctgg aagatacccg ctgtttcgag
    19501 tattctgagt cggttcaccg attagataaa ttccgtttcg ttgagtttgc ccgacgccga
    19561 aaagtgtaaa tagttcaaaa gaaagcaaat tgttggcatc tgtccacttt ggcgctatga
    19621 taggatcaca aagggggcat ttttaatgga taaaccaata agaccagaaa aagaagaact
    19681 gaaagaaaag ttatcaccca tggcttatgc agtcacacaa gaaaatgcaa cagaacgacc
    19741 atttagcggg aaatatgatg atttttacga aaaggggatc tatgtagata tcgtcagcgg
    19801 tgaaccgtta ttcagctctg cagaaaaata tgatgcaggt tgtggctggc catcattcag
    19861 caaaccaata acgaaaagac aagtgcgcga aaaagccgat ttttctcatg gcatgcatcg
    19921 aatagaagtt cgaagcaaag aagcagattc tcatctcgga catgtgttta ccgatggacc
    19981 agtagatcaa ggtggtctac gttactgcat caattctgcc tcactgaaat tcattccata
    20041 tgaaaagatg gatgaattag gttatgggga attcaaatca cttgtagaat aatggaaata
    20101 cacaaagagc ggtacaatac ggggatttac tcgatcgtcc gctctttttt tattcaagcg
    20161 attgctattt gaaagaaacg ctccctagac tccttttttc acaaggtgaa atgtgctaca
    20221 ataaagggaa cgtatgtgtg caaaagaaag aggactagaa tatgtatgaa tatctgacag
    20281 gtgtggttac ctttatcaac ccctattaca tagtagtaga aaccaatggt atcggctacc
    20341 agatcgcact aggaaatcct taccgctaca gtagcaaact aaatcaaaaa ataaaaattt
    20401 atgtccatca ggtaatacgt gaagatgcgc atcttctgta tggatttgat tcgctagaag
    20461 aaaagcaact gtttttgcga ttagtaagtg tttcaggaat cggtccgaaa agtgcccttg
    20521 caatcatggc aagcgaagac cactccggtt tgattcaagc agtagaatcc agtgatgtca
    20581 cttatttgac gaaatttcca ggagtaggga aaaagacagc acaacaaatg atcttggatc
    20641 taaaaggaaa attcggtgag ctgagtatcg atacaccttt tagtctattt gatgaagcaa
    20701 acacaaaaga cgctacagct ctatctgaag caatagaagc tctctcggct cttggttaca
    20761 gtgatcgaga aatcaaacgt gtagaaaaac aattaaaaga agtagataat cagacaacgg
    20821 atgagtatct acgccaagca ctcaagctaa tgatgaaaaa ataaggagga gaccaaatga
    20881 cagatgaata cttgttatca ccagaatctg gcgaaaatga actgagtttg gaaaaatctc
    20941 ttcgaccaca gctgctttct caatatatcg gacagagaaa agtcaaacaa gaacttcaga
    21001 tttacatcga agctgccaga aatcgggaag aacctttaga tcatgttctg ttgtacgggc
    21061 ctcctggatt aggaaaaacg acgatggcca tggtcattgc taatgagatg accgtaaata
    21121 ttcgtacgac gagtggccct gctatcgaac gtccgggtga cttagtagca atcttgaatg
    21181 aactggaagc aggggatgtc ctgtttatcg atgagatcca tcgattacct agagtggtag
    21241 aagagatgct ttattcagct atggaagatt tctatgtaga tatcatggta ggacaagggc
    21301 caaccgcaca tccggttcat ttccctcttc ctccctttac tttgattggc gcgacgacac
    21361 gggcaggtat gctttctgca cctttacgag atcgatttgg catcatttct catatggaat
    21421 attatgacga acaggatctg caggagatcg ttttacgttc ttcggatatt tttcaaacag
    21481 agatcattga agaaggggca ttggaaattg ctcgccgatc acgaggaacg cctagaatcg
    21541 ctaatcgctt gctcaaacgg gttcgtgatt ttgcgcaagt ccaaggagac gggaaaatcg
    21601 atcgtcctat agcggatcag gcacttactt tgctacaggt cgatcaggct ggattggatt
    21661 atgttgacca gaaattgttg cgaacaatga tcgagttata tggcggcgga ccagtagggt
    21721 taagtacgct ttcagtaaac ataggagaag agcgtgagac ggtagaagat atgtatgaac
    21781 cgtatctgat ccaaaaagga tttttaaagc gtacacctag agggcgaatt gctactgctt
    21841 atgcctatga acattttggt tacgactatc atatatgatt tttgctttta gattggagga
    21901 aaatgaatga gtgatacaac acgtacgaaa caattgatca aaaacgtatt gattgatttg
    21961 tgccaaacgc aatcttttcg aaaaatcagc gtccaaaaaa tcgcaaacga agcaaaaatc
    22021 aatcgacaaa cattttatta tcattttaca gataaatacg atttgcttcg ttgggtctat
    22081 tgtgaggatg cattgcatta tttagatcca caatgtcttt cacttgataa ttgggaagag
    22141 caagcattga aaatgctgaa agcaattgct gagaagaaag aattttatgc aaatactgtg
    22201 ctttctgacc cagaaatttt acaaaaagag ttttctcaac tgatccaaaa atcatttatc
    22261 aagatttttg aacaaatgga tgaagaaaga caactttctg caagagacaa actattttat
    22321 gctcgttttt tttcttacgg ttgcagtggt gtattagtca attggatcac gaatgaatat
    22381 gaagaaacac cgatggaaat cgctatgcag cttttccgtt tagccaagga tacggaatta
    22441 tattcttatc gtctttatgc aaaagaagag ttggaagaac agtaatagaa aaattttttg
    22501 ttggaaaagc aataatcgct aaaaggagat ggggaaatga caaaaatttt atttgtttgt
    22561 ttgggaaata tttgccgttc accgatggca gaaggcttgc tgagaaaacg aatagcatta
    22621 gaaggaaggg aagcagaatt ttttgtggat tctgctgcta caagcacgta tgaagttgga
    22681 aagacaccgc accctggaac gaaaaaaata ttaaaccaag aaaaagtaga tatgacgaat
    22741 atggttgctc ggcaaatcac accccacgat tttgaaactt ttgattggat cattggcatg
    22801 gatcaagaaa atgtagaaga actgaaacga agagcaccac gctcagcgca ggggaaaatc
    22861 catttgtttt taagtagcat tcctggaaaa gaaaaagaaa atgttcccga cccatactat
    22921 accaataact ttgatgagac atatcacttg atcacagaag gcctgaatca ttggtggaat
    22981 atttggattg atgaaaataa aataacaaat aatttgtaaa gttaacgctt tctttttaag
    23041 gcgatgttag agcatttcct tgtttttttg taaaatgtta ggtaaaataa aattaattaa
    23101 gtaacacttt cttagctaaa ggaggagtgc attcgatgaa cgaagagaca gtatcgccaa
    23161 tgaagaagct tacgaagtta gttactcata cttcgtatca actttttgat tcttttgtaa
    23221 atgacactga agaacatgct aagaatgtac gattgcaagg aaagtttatt cttgagctgc
    23281 aaaaagctgc tactgaacat agtctagtta tcttacaagt aagagcaaat cgtttgagta
    23341 gcaagtatga aacgatctca ggctgggtgg cgacaaaaac ggtcgaaaaa gaccgagtga
    23401 tgatccgcct acaaaacgaa gagcagcaga tccggatggt tcctgtagaa caaattgaaa
    23461 aaataacaat cctttcacca gcaggggaac aggaacggtt atcaaaataa tttataccag
    23521 atagaaaagc acgtatgagc ttgtaccatc aatcattact aatcaaaaat aaccgaatgc
    23581 tttaagctga tatcagtaaa acggaacgtt ttaggatgag tatcatagca tgacagcatt
    23641 cggttgat
//
LOCUS       DACEEZ010000039        23056 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20313_2017
            SAMN12639966-rid9879223.denovo.039, whole genome shotgun sequence.
ACCESSION   DACEEZ010000039 DACEEZ010000000
VERSION     DACEEZ010000039.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639966
            Sequence Read Archive: SRR10023158
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 23056)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23056)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741683.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741683.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:44:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,016
            CDSs (total)                      :: 2,962
            Genes (coding)                    :: 2,889
            CDSs (with protein)               :: 2,889
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 36 of 73
            Pseudo Genes (incomplete)         :: 29 of 73
            Pseudo Genes (internal stop)      :: 18 of 73
            Pseudo Genes (multiple problems)  :: 8 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23056
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639966-rid9879223.denovo.039"
                     /isolate="E20313_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            107..970
                     /locus_tag="IWO89_001550"
     CDS             107..970
                     /locus_tag="IWO89_001550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HAQ0456298.1"
                     /translation="MYQIVTDSCCDLPYHVLAKEKVDFLSMNLQLNDQELVDDLGKTF
                     NYDRYMNQLKNGAMPTTSQINVGRYLEFFRSYAEKQIPVLYVAFSSGLSGSYSSALQA
                     VELLKEEYEDPEIYVFDTKAASIGEGLLVLEAVRLKKEGKSIEETLEWLESNYLKVHS
                     WVTVDDLKYLERGGRISKASATLGSLLSVKPIITVDKEGRLTNVDKVRGRNKSIQTIV
                     NETVKTIVAPLDQIVYVAYAGDLESAEKAKRLLEEQIKMKDVKLYPLGPTIASHTGYG
                     CIAIFSMGISR"
     gene            complement(1099..2721)
                     /locus_tag="IWO89_001551"
     CDS             complement(1099..2721)
                     /locus_tag="IWO89_001551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016184106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAQ0456299.1"
                     /translation="MITVNDVSLHFSDRKLFDDVNIKFTPGNCYGLIGANGAGKSTFL
                     KVLSGDLQPSTGSVTMGPDERMAVLKQNHYDYEDYTVLETVIMGHKRLYEVLKEKDAI
                     YMKEDFTDEDGIRAAELEGEFAELNGWEAEPEAAVLLQGLNIPEELHHQQMSELTGGQ
                     KVKVLLAQTLFGKPDVLLLDEPTNGLDIQSINWLEEFLINFENTVIVVSHDRHFLNKV
                     CTHMADLDFGKIKLYVGNYDFWLESSQLAAKLQANANAKKEEQIKELQEFIARFSANA
                     SKSKQATSRKKMLEKITLDDIQPSSRRYPFVGFKPEREIGNDLLQVENVSVTIDGKKI
                     LDDISFNLSKEDKVAFVAENDITTTTLFKVIMGEITPDTGTVRWGVTTSNSYLPKDTT
                     KEFDTDLTILDWLRQYASKEEDDNTFLRSFLGRMLFSGEEVLKPVNVLSGGEKVRCML
                     SKMMLSKANVLVLDDPTNHLDLESITALNDGLMAFTGSILFASHDHQFIQTLANRIIA
                     VSDKGVIDRADTTYDEFLENADIQKQLNELWA"
     gene            2970..3491
                     /locus_tag="IWO89_001552"
     CDS             2970..3491
                     /locus_tag="IWO89_001552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0456300.1"
                     /translation="MDEKRTIDWGSLILGILFVLVSLLSFQDPVGNLVAIVVVFAIFA
                     FIKGIFELFVRNRLKELTGYKGKMPLIIGIIDILVGVFFLFNIGAGVAALPFVFAVWF
                     IADSVLALFTADLARGVSEGYYWFTIIVNILGILLGIFLLFNPISSALTLSFLVGFYF
                     MLFGITHIVYAFR"
     gene            complement(3677..5407)
                     /gene="spxB"
                     /locus_tag="IWO89_001553"
     CDS             complement(3677..5407)
                     /gene="spxB"
                     /locus_tag="IWO89_001553"
                     /EC_number="1.2.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate oxidase"
                     /protein_id="HAQ0456301.1"
                     /translation="MSKINAGIAMVKVLESWGIDHIYGIPGGSFNSIMDALYQEKTEI
                     NYIQVRHEEVGALAAAADAKLTGKVGAVFGSAGPGATHLINGLYDAQMDHVPLVAFLG
                     QVASTSMNYNSFQELNENPIFADVSVYNRTVMTPQSLPHVVDEAIKAAYEHKGVAIVT
                     IPVDLGFEEIDEQPFSTAHTHKTSVILPEEKDLLAALPYLEKAKKPVLYVGQGTRNGF
                     PQIKEFSEHFSVPVVASVLAKGIIPDDYENFLGFAGRVATKPGNEALAEADLILFVGS
                     DFPFGRAFFNPTAEFIQIDIDASKFGRRHETSLSILGDANTALQRLVELGDARPADSW
                     YLANQENKEKWVNWLKSFEDREEEPIRPEAVYKEINRIAEDDAIFVTDVGNTTIHSIR
                     LLNMNGKQKHTTSGWFATMGNGVPGGIAAQLSYPEKQVFTLSGDGGFAMVMQDIITQV
                     KYQLPIINVVFSNDSFGFIEAEQEDTEQKKFGVFLEDADFGKVGEALGANSFTVTEYS
                     QLRFAFDAAAKSDRPVVIDVKINNKRPLPVEDLHLDPRKYSEEEIQAFKEKYEVHDLP
                     VLNELYEKRL"
     gene            5572..6390
                     /locus_tag="IWO89_001554"
     CDS             5572..6390
                     /locus_tag="IWO89_001554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005232914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ZIP family metal transporter"
                     /protein_id="HAQ0456302.1"
                     /translation="MVEWLLRLEPWQQALAGTAFTYFMTALGAGLVFFFKEIKKEVLN
                     LMLGFASGVMIAASFWSLLDPAITKAEENGDIAWLVVSIGFGLGGVFLYMADKTLPHM
                     HFGPQHEAEGLPTHLKRTILLVFSITLHNIPEGLAVGVAFGAAATADNPTAAILAAVS
                     VALGIGIQNFPEGAAVSIPLRQEGLSRKKAFVYGQASGIVEPIAGVIGALLVTKVELL
                     LPYALAFAAGAMIYVVVEELIPEAQQTLSSKRHFAVFGVMSGFIIMMILDVALG"
     gene            complement(6493..6690)
                     /locus_tag="IWO89_001555"
     CDS             complement(6493..6690)
                     /locus_tag="IWO89_001555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0456303.1"
                     /translation="MRQKNMFLNIFMVLLVLIAASFVISLFVSVLSGLLWFAVKVLIP
                     IALIIWLVRWIKGNTHRKNYY"
     gene            6859..7437
                     /locus_tag="IWO89_001556"
     CDS             6859..7437
                     /locus_tag="IWO89_001556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1211 domain-containing protein"
                     /protein_id="HAQ0456304.1"
                     /translation="MPKNRLEAFTDAVIAIIMTILVLELHEPNGDTWEALAALGYRFF
                     IYIVSFIILAIYWNNHHHLFQVVHKINGRVLWANSFFVFALSLFPFATSWISEYINSL
                     APEMTYGFVTLTANIAYYILAKELVKANKGHEKALKAMLNNYKKSYLSIGLNVLGIVL
                     GYLVAPVAVLIANIVILAAWIIPSRTIESQYR"
     gene            complement(8482..8985)
                     /locus_tag="IWO89_001557"
     CDS             complement(8482..8985)
                     /locus_tag="IWO89_001557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HAQ0456305.1"
                     /translation="MRYVSKFLLGIYLFFLLWLILFKLSFNLPQFLTYSYSNVNLVPF
                     STFSFENTTVLRETTYNLIVFFPFGVLLNVNFKRLSFSKKLGIIFLVSFLAELIQFLF
                     GIGVADITDLITNTTGGLIGLWAYQLLNKHLSTNKLDRLAIILGYILFLFLIVAFLFL
                     TRTVRLQ"
     gene            9344..10369
                     /locus_tag="IWO89_001558"
     CDS             9344..10369
                     /locus_tag="IWO89_001558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HAQ0456306.1"
                     /translation="MFKTYFRFNKIQDNPFRIMNLMLLIFIVLWLGYINHDFLISSFS
                     SLGIFTFFYYQNIPMKYLLKRMFLIGCGLLVAFVLGILSTYVLWLEPFAVALVAFSSR
                     FVLRLFHISKPGGLFFAMLSAMGTSMQLPIAQLPIVSLYFFMGVVFALIAAVITKLLD
                     SRPEQTIEKATLKERFHEEPLVIIDSVFYSAALFLSVYVSHGLNLHNPYWLTLSCASI
                     LLAENLDAMKHRQVQYLIGSMGGLCVSAFLSFVPFTQLQTIFLITFLYGIAQFLVARN
                     YAVANIFLNPMALMLSTLIRGAYLISLIEYRFLGIVIGSFIGLGVAWVMTVGLQHYLT
                     VVRQKLE"
     gene            10692..11570
                     /locus_tag="IWO89_001559"
     CDS             10692..11570
                     /locus_tag="IWO89_001559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0456307.1"
                     /translation="MRFKDMKRGNLMKKISNEIQILAETLTLPKVVIEQMIVQAKNIL
                     DEVFEQYSHELMDPNKAKATAKKIDKVYEEEPFAALAIYLYAASQSWKKIYQPLKIPR
                     TIYLATMNAFTRFIHEHFQVTGSYRFDRGFWIWHYTSGLIFRIGELEYEMTYFPKGYT
                     DLRLEGKQCLSLHIPSDADLSQNKIRDSYESAIRFFELYFPDYHYQVMYTDTWLLSPN
                     LTKWLKKESKICLFAADYRLLSVDEQDDSGVPWIFGRVDAQIHDYPESTSLQRQAKEQ
                     LLAGEHIGSGLGILTS"
     gene            12046..12162
                     /locus_tag="IWO89_001560"
     CDS             12046..12162
                     /locus_tag="IWO89_001560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002383181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative metal homeostasis protein"
                     /protein_id="HAQ0456308.1"
                     /translation="MEKTDISSAYRRLKSPNIKTRKRALKIIKDVKRNSGKR"
     gene            13090..13428
                     /locus_tag="IWO89_001561"
     CDS             13090..13428
                     /locus_tag="IWO89_001561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0456309.1"
                     /translation="MTQPVSSEEHDNLFRATEEFKKYQENIVPETPLASEPITDIDGN
                     QIGWYIQYEVMVVNNDSIVKNTVGLELTPIEKVSSLLTYIYTDESDETEIILTDYSKI
                     ATDKKYILWM"
     gene            13522..13752
                     /locus_tag="IWO89_001562"
     CDS             13522..13752
                     /locus_tag="IWO89_001562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0456310.1"
                     /translation="MLVEKGKENIYYVNVAKVREDENEWKEFKSRYSINSTPTFTVYR
                     EGSIEKTVFWTKESGMSLAEVEEFLDYVSMQQ"
     gene            14152..14523
                     /locus_tag="IWO89_001563"
     CDS             14152..14523
                     /locus_tag="IWO89_001563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cro/Cl family transcriptional regulator"
                     /protein_id="HAQ0456311.1"
                     /translation="MRDYVTDKTQEVINQSQSLNELSDKAKEEAVAIISSGISDYIDM
                     SGEINYGDKDKIVRMIKAFIKTFDVKADLKFNDTYLVGKLINILGTEKDTETFISSLS
                     MLFTDKHFSTQLGGEELVEIF"
     gene            complement(14797..15489)
                     /locus_tag="IWO89_001564"
     CDS             complement(14797..15489)
                     /locus_tag="IWO89_001564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HAQ0456312.1"
                     /translation="MKGIIFDFNGTMFLDSHLHEAAWLHMIHDHTKDGLSDEDILKNI
                     HGRTNTEILKHFISEHLTASEIAVLSEEKESYYRMLCLQNENELQLTKGLEATLDQLT
                     DQQIPLTIATATVKENVAFYFDIFDLARWFDWDKIVYDDGSFPGKPQPDIFLKAADKL
                     SLAPADCLVIEDAYSGLLAAKRAGIGTIIAIDPFGKNKTVFEQAQLNKDGTIKDFTGF
                     WTEHLSATSVNS"
     gene            complement(15492..16277)
                     /locus_tag="IWO89_001565"
     CDS             complement(15492..16277)
                     /locus_tag="IWO89_001565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HAQ0456313.1"
                     /translation="MLKKERLLTIVEMVNKKGILTVNEIINQLDVSDMTVRRDLDELE
                     KSGKLLRVHGGAQSLSYTLDQELSHTEKATLQIQEKKEIAATAASLIHEGETIFLGPG
                     TTIELLATHLLNKKIRIITNNYPVFDILAQADGPEILLIGGDFRKNTGAFVGPITNDN
                     LRRLKFTKAFISSNGVHNEAISTYSTEEGEAQQIALNNSRKKYLLVDNKKFNRDDFYV
                     FYNLHDFDLLITDRQISREVQEHYAQYVDLQIADSTENTTIGA"
     gene            16514..17914
                     /gene="lacG"
                     /locus_tag="IWO89_001566"
     CDS             16514..17914
                     /gene="lacG"
                     /locus_tag="IWO89_001566"
                     /EC_number="3.2.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-galactosidase"
                     /protein_id="HAQ0456314.1"
                     /translation="MLKLPEDFIFGGATAAYQVEGATKEGGKGAVAWDDFLEEQGRFS
                     PDPASDFYHQYAKDIELCERFGVNGLRLSIAWSRIFPDGAGKPNPEGIAFYHRVFEEC
                     KKRNVTPFVTLHHFDTPKRLFDHGDFLNRETIEAFVSYAIFCFHEFKEVKVWSTFNEI
                     YPVATNQYLLGVFPPGIKYDFTKIVACLHNMMVAHARVVNYFKENELPGEIGVVHSLE
                     TKYAATDAPEDKHAAFLDDALSIRFLLDATYLGYYSTETLTALDEICEANQASYHFPE
                     EDFVELKKASTRNDYLGINHYQCHFVKAYDGENAIHHNGTGEKGTSVYKVKGIGERIY
                     KEGIPRTDWDWLIYPEGLYDLLLRIKSDYPHYNKIYITENGMGYKDQFEDGIIMDQPR
                     IDYLRVYLESLSKAITAGVNVKGYFLWSLMDLFSWTNGYNKRYGLFYVDFETQKRYPK
                     ESAYWYKLVSETKTII"
     gene            17939..18256
                     /locus_tag="IWO89_001567"
     CDS             17939..18256
                     /locus_tag="IWO89_001567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010749967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HAQ0456315.1"
                     /translation="MNKEELQMLGFEIVAYSGDARSTLLKLLKEVRQGNFEHVDSALK
                     EADENLTLAHNSQTKILAEEASGKEMEMGFIFIHGQDHLMTTLLLRDLIQDFIVLYRQ
                     NQG"
     gene            18269..19942
                     /locus_tag="IWO89_001568"
     CDS             18269..19942
                     /locus_tag="IWO89_001568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAQ0456316.1"
                     /translation="MDGIIKQIEKGKPFFEKISRNSYLGAVRDGFLTAMPAILFSSIF
                     ILIAAVPEIFGYQWPESVSTWLWKVYNYSMGVVGLLVSATTARCLADSMNRRLPKNKV
                     INTTSVMLASVVSFLLLSVSPIEGGFSTEYMGTKGLLASFVAAFLTVNLYKFCVLKDI
                     TIKMPKEVPGTISQTFRDIFPFSFSVFAAVLIDLGIRQIFQISFAEAIITVLQPLFTA
                     ADGYLGIAIIWGAMALFWFVGVHGPSIVEPAIAAIIYANVETNLQLFNSGEQASKVLT
                     VGLGNFVGTMGGTGATLVVPFIFLLFAKSKQLKAVGKASFIPVSFAVNEPLLFAAPMI
                     LNPYFFIPFLLTPVVNVCLFKFFVDVLGMNSFMYVLPWATPAPIGLVLGTGMGILAFV
                     LAAVLIVVDFLIYLPFCKAYDAVLVEKEAQKAEVIATEEGASADVREEVPVAASQVIS
                     DANKEINVLVLCAGAGTSAMLANALKKGASESKAPISAMAGAYGSHYEMLQDFDMVVL
                     APQVNSYYDDIKEDTDRLGIKLVATKGAEYIGLTRDPEKAVQFILNQFQ"
     gene            19956..21587
                     /locus_tag="IWO89_001569"
     CDS             19956..21587
                     /locus_tag="IWO89_001569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016608433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinogalactan endo-1,4-beta-galactosidase"
                     /protein_id="HAQ0456317.1"
                     /translation="MKMGIWLRKAVLFAAVLGALGAVSKTVEASASEFIRGADISILS
                     DMEKSGAKYYEDDISKDALEILKNNGVNYVRLRLWQDPYNSNGESYGAGTNDLETTID
                     LAKRAKNLGLKVLLDFHYSDFWVDPGKQNLPKAWQGLTFEEMNTALYDYTKNVLSEMK
                     QLDVYPDMVQIGNELNSGMLWPYGKSWGEGGGEFDRLAAFLKSGIQAVRDTQPKTTPV
                     MLHLADGGDTGAFTWWFDEITSRGVSFDLIGVSYYPYWHGSMNELQTNMNAISQRYGK
                     EVIVVETAYGHTTANADTMPNAFGEAEAAAGGYEPTPTGQAEYLLDLADRIQAVPNNR
                     GAGFFYWEPLWYNGNVSWATQAGMNYLSVQSTMGNEWDNQAMFDFAGNALPSLRAFKQ
                     AGAQINLVKNASFESDGPTTSPSNWQTWFQQGTAKGTIKTEAGTFFGDYKLTFWQDQA
                     YEASAYQNVSGISKGTYTLSAYVMSGGEFDTNQLYIKNYGGAELNTAVVKTSEWTKIT
                     IKDIPITTGVCEIGVYSKASGNAWTNVDFVSLVKQ"
     gene            22459..>23056
                     /locus_tag="IWO89_001570"
     CDS             22459..>23056
                     /locus_tag="IWO89_001570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000997693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256-like element ISLgar5 family transposase"
                     /protein_id="HAQ0456318.1"
                     /translation="MNDFTTEILKTLANKGDLNELFRVHLEKAVNTLLKTELTAFLDY
                     EKYDRIGFNTGNSRNGSYDRTVKTEYGELHLQIPRDRNGEFKQQTVPAYRRTNDTLEE
                     TVIHLFRKGITMSEIADLIEKMYGHYYTPQTMSNITKSFTEEVTAFNGRELHDRYAAI
                     YMDATYIPLKRKTVAKEAIHIAVGIRPDGSKEVLSYAIA"
BASE COUNT         7208 a         3881 c         4663 g         7304 t
ORIGIN      
        1 ttttgattag aacttgcgaa aataaaccgg ttaaagtgtg tctagcgcaa gagatttgct
       61 ataatagacg ataagggtaa aatgtaacga gaaggggttg aaaaatgtgt atcaaattgt
      121 aacagattct tgttgtgatc tgccatacca tgtattagca aaagaaaaag ttgatttttt
      181 atcaatgaat ctccaattga atgatcaaga gttagtagat gacctcggaa agacattcaa
      241 ttatgaccgc tacatgaatc agctgaaaaa tggtgcgatg cctacgacat cacaaatcaa
      301 tgttggccga tatttagaat ttttccgttc ttatgcggaa aaacagattc ctgttttata
      361 tgtggctttt tccagtggat taagcggctc ttacagtagt gcgcttcaag cggttgaatt
      421 gctgaaggaa gaatatgaag accctgagat ctatgttttt gatacaaaag ctgcaagtat
      481 tggggaaggg cttttagtat tggaagctgt aagattgaaa aaagaaggta aaagcatcga
      541 agaaacccta gagtggctgg aaagtaatta tttgaaagtc cattcatggg taacagtaga
      601 cgatctgaaa tatctagaac gaggcggtag gatttccaaa gcaagtgcta ctcttggcag
      661 tctattgagt gtaaagccta ttattacggt ggataaagaa ggaaggctga cgaatgttga
      721 caaagttcga ggaagaaaca agtcgatcca gacaatcgtc aatgagacgg taaaaacaat
      781 cgttgcacca cttgatcaaa tcgtttatgt ggcttacgct ggggatctag agagcgcgga
      841 gaaggcaaaa cggcttttgg aagagcagat aaagatgaaa gatgtgaagc tttatccctt
      901 aggaccaacg atcgccagtc acacaggcta tggctgtatc gcgatttttt cgatgggaat
      961 cagtcgataa aagcagtaac tcattctgta aaaaagccca gaaattgatt gtaacaaaaa
     1021 cagcgactct taaaagatag atgagcatcc tatctcttag aggtcgctgt ttttttattt
     1081 tttaagctaa ataggccatt atgcccataa ctcgttcaat tgtttttgaa tatctgcatt
     1141 ttccaaaaat tcatcatatg tcgtatcagc tcgatcgatc acacctttat ccgaaacggc
     1201 aatgatgcgg tttgccaatg tttggataaa ttgatggtca tgtgacgcaa ataagattga
     1261 gccagtaaat gccatcaagc cgtcattcaa tgctgtgatt gattctaagt ctaaatggtt
     1321 cgttggatca tctaatacaa gtacgttagc tttcgaaagc atcatttttg aaagcatgca
     1381 acggactttc tctccaccag ataaaacatt cactggtttc aatacttctt caccagaaaa
     1441 caacattctt cctaagaagc ttcttaagaa ggtgttatca tcttcttctt tacttgcata
     1501 ttgacgcaac cagtctaaaa tcgttagatc agtatcgaat tctttcgttg tatcctttgg
     1561 taaataagaa ttacttgttg tcacacccca acgtactgtt ccagtatctg gcgtaatttc
     1621 acccatgata actttgaata gcgttgttgt tgttatatca ttttctgcga cgaaagcgac
     1681 cttgtcttct ttgctcaaat taaaagatat atcatctaaa attttctttc catcaatcgt
     1741 tacacttaca ttttctacct gcaataagtc gtttccaatc tcacgttcag gtttgaatcc
     1801 aacgaatgga taacgtctag aagaaggttg gatatcgtct aaagtaattt tttcaagcat
     1861 ttttttacgt gaagttgctt gttttgattt tgatgcattc gcactaaagc gggcaataaa
     1921 ttcttggagt tctttgattt gttcttcttt tttggcattt gcgtttgctt gaagtttagc
     1981 cgctagttga ctagattcca accaaaaatc ataatttcct acataaagtt tgattttacc
     2041 aaaatctaga tccgccatat gtgtacatac tttgttcaaa aagtgacggt catgggatac
     2101 aacgatcaca gtattttcaa aattgatcaa aaattcttcc agccaattga tcgattggat
     2161 atccagaccg tttgttggtt cgtctagcaa aagaacatca ggtttaccaa atagtgtttg
     2221 tgctaataac acttttacct tttgtccacc agttaattca ctcatttgct gatggtgcaa
     2281 ttcttcaggg atgttcagac cttgcagtaa aacagctgct tctggttcag cttcccatcc
     2341 attaagctca gcaaattctc cttctaattc agctgctcga ataccatctt cgtcagtgaa
     2401 atcctctttc atgtaaatcg catctttttc tttcagcact tcatacagac gcttgtgtcc
     2461 catgatgact gtttcaagta cagtatagtc ttcatagtcg taatggttct gtttcaatac
     2521 ggccatccgt tcatctggtc ccatagtaac ggaacctgtg gaaggctgca gatcccctga
     2581 taacacttta aggaacgttg atttacctgc accatttgca ccaatcaagc catagcagtt
     2641 tccgggggta aatttaatat tcacatcatc aaatagtttg cggtctgaaa agtgcaaact
     2701 cacatcattt acagtaatca atttttttcc tcacttttat cattattagt tttgacaagt
     2761 cttatagaat tatatagggg aaaggtaaaa gattcaaggc aatcgaacat tctttgtgtt
     2821 tcacgtgaaa cagaatgaaa aaaataaaaa taaaagcgga gcgaaaatag ctactatagg
     2881 atttattagt ttgcaacagc ataatttttg acgttattta taagagggag tactataaaa
     2941 ctgtaaaaga aatgaaagaa ggattgaaaa tggatgaaaa aagaaccatc gactggggct
     3001 cattgatttt gggaatttta tttgtacttg tctcattgtt atcatttcaa gaccctgtcg
     3061 gaaatttagt tgcaattgta gtagtctttg cgatttttgc ctttattaaa gggatatttg
     3121 aactgtttgt acggaacaga ttaaaagagc taacaggata taaaggaaag atgcccttaa
     3181 taatcgggat catcgatatt ctagttggcg tctttttctt attcaatatt ggtgcaggag
     3241 tcgcagcatt gccttttgtt tttgcagtat ggtttattgc tgattcagtt ttagcgttat
     3301 ttactgctga cttggcaaga ggagtaagtg aaggatatta ttggtttacg atcatcgtca
     3361 atattttagg aattttatta ggaattttct tattgtttaa tccaatttct tctgcgttga
     3421 cattaagctt tttagttgga ttctatttta tgttatttgg tatcacacat atcgtctatg
     3481 catttagata agtaaaatca aacaaataaa aaagccggct tccgattaca gttttactgt
     3541 tgtaattgga agccggcttt tttatcatgg acaaatatgc acgaggtgat ttttaggtgt
     3601 cgagacaaat ttatgcaaaa atgtgagaag ctatatacac aaattttatc ttaatactcg
     3661 aagcatagcg cagttgtcac aacctctttt catataattc attcaatact ggcaaatcat
     3721 ggacttcata tttttctttg aatgcttgga tttcttcttc agaatatttt cttggatcta
     3781 aatgcagatc ttctactggc aatgggcgct tgttattgat cttcacatcg attactacag
     3841 gacgatctga ttttgcagct gcatcaaagg caaatcgaag ttgcgagtat tctgtgactg
     3901 taaagctatt tgccccgagc gcttctccaa ctttaccgaa atctgcatct tctaaaaata
     3961 caccaaattt cttttgttcc gtatcttctt gttctgcttc gatgaaacca aaagaatcat
     4021 tcgaaaagac aacattaata attggaagct gatatttgac ttgtgtaatg atatcttgca
     4081 tgaccatagc aaacccgcca tcaccactta aggtaaatac ttgtttttct gggtagctta
     4141 gttgtgcggc aattcctcct ggcacaccat tacccatagt tgcgaaccat ccagatgtcg
     4201 tatgtttttg tttaccgttc atattcaata aacgaataga atggatagtc gtgttaccga
     4261 catctgtgac gaaaatcgca tcgtcttcag caatacgatt gatttcttta tagacagcct
     4321 ctggacgaat cggttcttcc tctcgatctt caaaagattt caaccaattt acccattttt
     4381 ctttattttc ttgatttgct aggtaccagc tatcagcagg gcgagcatcc cctaactcga
     4441 caagtctttg caaagctgtg tttgcatcac cgaggatcga caaagatgtt tcatggcgtc
     4501 gtccaaattt tgacgcatcg atatcgattt ggataaattc agcagttgga ttaaaaaatg
     4561 cacgtccaaa tgggaagtcg ctaccgacaa ataagatcag atcggcttct gccaatgctt
     4621 catttccggg tttagtagcg acacgtccag caaaaccaag aaagttttca taatcatcag
     4681 gaataattcc ttttgctaga acggaagcga caacaggaac tgaaaaatgt tctgagaatt
     4741 ctttgatttg cggaaatccg tttcgcgttc cttgaccaac ataaagaact ggtttttttg
     4801 ctttttcaag ataaggcaaa gcagctagca aatctttttc ttctggtaat atcacacttg
     4861 ttttgtgagt gtgagcggtg gagaaaggtt gttcgtcaat ttcttcaaaa ccaagatcaa
     4921 cagggatagt cacgatagcc acgcctttat gctcataggc ggctttaatg gcttcatcta
     4981 ctacatgcgg aagactttgt ggtgtcatga ctgtacgatt gtatacgcta acatctgcga
     5041 aaataggatt ttcatttagt tcctggaaag agttatagtt catggatgta gaagcaactt
     5101 gtcctaagaa cgctactaga ggcacatggt ccatctgtgc atcatacaaa ccattgatca
     5161 aatgtgtcgc accaggtcct gcagagccga agacagcgcc gactttcccc gttaattttg
     5221 catctgcagc ggctgcgagt gcgccgactt cttcatgacg aacttgaatg tagttgattt
     5281 cggttttttc ttgatatagc gcatccataa ttgaattgaa agatcctccg ggaatgccgt
     5341 aaatatggtc gataccccag ctttcaagaa cttttaccat tgcgattcct gcattgattt
     5401 ttgacatggt gtttcactcc tttagcaatg atgatacata aattttttgt atctagagta
     5461 tagaactttt tatcgaaaat cccaactaat tcgtcttttc acaaaaatgt agtcatgttt
     5521 ttgggaatat gctagagtag tacatagata gattcaagag aggggaatgg catggttgaa
     5581 tggctattga gacttgaacc ttggcagcaa gcacttgctg ggacagcttt tacttatttt
     5641 atgacagctc ttggtgccgg gttggttttt ttctttaaag aaatcaaaaa agaagtgcta
     5701 aacttgatgt taggatttgc ttctggtgtc atgattgctg caagtttttg gtctttgtta
     5761 gatccggcta ttacaaaagc agaagaaaat ggagatattg cttggctagt tgtaagtatt
     5821 gggtttggtt taggcggtgt gtttttatat atggcagata aaacactgcc ccatatgcat
     5881 tttggaccac aacatgaagc agaaggattg cctacccatt taaaacggac gatcttgctt
     5941 gttttttcta ttacattgca taacattcct gaaggtttag ccgtgggggt agcattcggt
     6001 gcagcagcaa ctgcggataa tccgacagcc gctatattag ccgctgtttc ggtggcgcta
     6061 ggtattggta tccaaaactt cccagaagga gcagctgtat caatcccgtt gcgacaagaa
     6121 ggtctgagtc gtaaaaaggc ctttgtttac ggacaagctt ctggaatcgt tgaaccaatt
     6181 gctggagtga ttggagcgct actagtaacg aaagtggagc tgctgcttcc ttatgcactt
     6241 gcttttgccg caggagcaat gatttatgtc gtggtggaag aattgatccc tgaagcacag
     6301 cagacattgt ccagcaaacg tcattttgca gtttttggcg tcatgagtgg atttatcatt
     6361 atgatgattt tggatgtggc attaggataa ataaaaaaaa tatatatgat atatcaaata
     6421 gggccgcgaa cttatatcgc gaccctattt ttttagtaag ttatatcata atgaagtgaa
     6481 tggtatcata cattaataat agttttttct atgtgtattt cctttgatcc agcgaacaag
     6541 ccagatgata agtgcgattg ggattagaac tttgaccgca aaccataaaa gcccgcttag
     6601 gacactgaca aaaagactga tgacgaagct agctgcaatt agcactaaaa gaaccatgaa
     6661 gatatttaag aacatatttt tttgacgcat acaaaaccct cctcttgttc attctttact
     6721 atagaaaaag tataacaggg attgtttaaa aacacctcca actgaagact gatttttact
     6781 tcatctactt aaatgatcaa acaattgctt tttctctggt ttatcatcta aaatagagcg
     6841 gaaaaggagg agaatatcat gcctaaaaat cgtcttgaag catttacaga tgctgtaatc
     6901 gcgattatca tgactatttt agttttagaa cttcatgaac ctaacggaga cacgtgggag
     6961 gcgctcgctg cattaggtta ccgatttttt atctatattg tcagttttat cattttagcg
     7021 atttattgga acaatcatca tcatttattt caagttgtac ataagataaa tggacgggtc
     7081 ttatgggcga acagtttctt tgtttttgcg ctttcgcttt ttccgtttgc aacttcatgg
     7141 atcagtgagt acatcaatag cctggctcca gaaatgactt atggttttgt taccttgacc
     7201 gctaatattg cgtattatat cttggcaaaa gaacttgtca aagcaaataa aggccacgag
     7261 aaagcattga aggcgatgtt gaataattac aagaaatcgt atctatcgat tggtttgaat
     7321 gtgttaggaa ttgtcttagg gtatttagta gcgccagtag ccgtgctgat cgcaaacatc
     7381 gtgattctag ctgcttggat catcccaagt cgtaccatcg aatcacaata tcggtaaaat
     7441 gatccgtgtt taaaatttaa gagaaaaata tagaaaaaat aaataaacga tcaaatatcc
     7501 gagtgatact agtattcaaa gagggatgca gatggatcaa aaaagaattc atgtaggact
     7561 gattactagt tttgtcacga tgcttattta tttggatcaa ggtcatttga cttggacagt
     7621 agttggtgtc gtaggatttt tgacattatt cacagcaata aaaaaataaa catctttatg
     7681 taatctgatg ttctatctat ttaagaaaag catgtttaca gcagtatgat tctgttgtaa
     7741 acgtgttttt atttgtggaa aatttaattt ttagaaaagt gtatcgaaaa atagatagtt
     7801 agtttttcca cagtggataa cttcgtaaga gataaaaata aaaaattagt caaagaaggg
     7861 cgtatagatt tcttttgata ggagatatac aacagaaatg aataaatatt aacgtgtttt
     7921 caaacagtat aaaacggtgt ggtttttgga ataaaaaaat aacatatctc attttacgct
     7981 tttgttattt tatacttaaa aaataacgac acagaaaata ccgtgtctca gatttctcta
     8041 ctacttctaa aaactactgt ttttttcctt tgaaatgcct tcaaaaagta ggatcaggca
     8101 gaatatactg gcagaatcaa ggagaaggga gcctaaataa ggagaaaaac catagaaaat
     8161 ataaagtaat tacttataaa acaagctttt tatagaaaaa taatatacct gagtagggta
     8221 taaattaaga cggaattcct ttctagattg agatattgac taggaagaat gttaagattt
     8281 taagccttta agctaaaaaa aaaccctttt tgttaaataa ggggaatata gcctgtcagt
     8341 ttcaaactgc aagtcgtcct gctgcaataa tatagtagat tagagatgaa aatagatttt
     8401 tttaaaccaa tgactgaata aaaagaccgt catagataca cattggaatg tgtctattac
     8461 ggtctttttt tacgaacgat tttattgaag acggacagtt cgggtcaaaa ataaaaaagc
     8521 tacgatcaga aataagaata aaatgtatcc caaaataatg gctagacggt ctaacttatt
     8581 tgtcgataga tgtttgttta gaagttgata ggcccataaa cctatcagtc ctcctgttgt
     8641 attagttatc aaatcagtga tgtccgctac gccaataccg aataggaatt gaatcaattc
     8701 tgccaagaag cttacaagga aaataatccc taatttttta gaaaaagaaa gtctcttgaa
     8761 attgacattc aaaagcaccc caaaaggaaa aaagacaatc aagttataag tggtctcacg
     8821 taataccgtc gtattttcaa aagaaaaagt agaaaaaggg acgagattga cattgctgta
     8881 agaataagtg agaaattgtg gaagattaaa tgatagcttg aataaaatca accataatag
     8941 aaagaataaa taaatcccta ataaaaattt tgatacatac cgcatagaca tctcctccat
     9001 ttaaaaagta taccatcgaa aaccgctgaa aaatcataaa aaagtatgaa gtttaaggac
     9061 caacgtagtt atcgtacgaa aattagaagc ttttttttgt tgggaatccg gtttctttaa
     9121 caaagctaag atagcgagtt gcttggaaaa gttgctatct tttttctgtc aaaacgactc
     9181 ggaatgttcg ttttttgttc taatttctcc aagttgttta ctttttgata ctaagtttat
     9241 gaaaaatcaa gaaatagttg ttatactagg taatgctgac aaacgggatt ggcatttcat
     9301 cttggttcag ctttttttcc aattactagg aaggagggat agattgttca agacttattt
     9361 tcgtttcaat aaaatacaag ataatccttt tcgtatcatg aatctgatgc tacttatctt
     9421 tattgtatta tggttaggtt acataaatca tgattttttg atttcaagtt tttcatcatt
     9481 agggatcttt acttttttct attatcaaaa cataccgatg aaatatttgc tgaaacgtat
     9541 gtttctgatc ggttgcggac tgttggttgc tttcgttttg gggatcttat cgacttatgt
     9601 tttatggctt gaaccatttg ccgtagcttt ggttgctttt tccagcagat tcgtgttgcg
     9661 attattccat atttctaaac caggtggctt gttttttgcg atgctttctg ccatgggaac
     9721 gagcatgcag ctgccaatcg ctcaattacc gatcgtctca ctttattttt ttatgggtgt
     9781 cgtttttgcc ctgattgcgg cagtgattac aaaattactg gattcacggc ctgagcagac
     9841 aattgaaaaa gcaactttaa aagaaagatt tcacgaagag cctttagtga tcattgattc
     9901 tgttttctac agcgctgcct tatttctttc cgtttatgtc agtcacggct tgaatctgca
     9961 taatccttat tggttgacct tgtcatgtgc atcgattttg ctagcagaaa acttggatgc
    10021 gatgaagcat cgacaagtcc aatatctgat tggatcgatg ggtgggttat gtgtgtcggc
    10081 attcttgtca tttgtgccgt ttacgcagtt gcagaccatt tttctgatca cttttctata
    10141 tggaatcgca caatttctag ttgctagaaa ttatgcagtt gctaatattt ttcttaatcc
    10201 gatggcgttg atgctatcta cacttattcg tggagcatac ttgatttctt tgatcgaata
    10261 tcgtttttta gggatcgtca taggaagttt catcggttta ggtgttgcgt gggtaatgac
    10321 ggttggtttg caacattatt taacggttgt tcgtcaaaaa ttagaataga aggacaaata
    10381 tatgtgccaa aagaggttat gacaagattt ttgtcacaac ctctaattca aggtgttcaa
    10441 aagccagatt ctcagcatta agtcaaattc acacaaaaat gtggaaagga actttcaaaa
    10501 aaattttata tttattctat caaaaaattt tgtttatgat ggtaactgaa taaactttcc
    10561 tgagatggtc aatgctttcc ttgaaagtct tacagatatt ctttcttcat agaaagttta
    10621 agttttatag ttagtgtaca ataaaagtat gtttatacca tatggtgtcc ggataatcag
    10681 tcatttaaac aatgaggttc aaagatatga aaaggggaaa tttgatgaag aaaataagca
    10741 atgaaatcca aattctggca gaaactttaa ctcttcccaa agtcgttata gagcaaatga
    10801 ttgtacaagc aaaaaacatc cttgatgagg tgtttgagca atatagtcat gaactaatgg
    10861 atcctaataa agcaaaggcg actgcaaaga aaatagacaa agtctatgaa gaggaacctt
    10921 ttgcagcatt agctatttat ctatatgcag cttctcaaag ttggaaaaaa atttatcaac
    10981 cattgaagat tccaagaact atctatttgg caacaatgaa tgcttttact agatttattc
    11041 atgaacattt ccaagtaaca ggaagctatc gctttgaccg tggtttttgg atttggcatt
    11101 acactagtgg cttgatcttt cggattggtg aattggagta cgaaatgaca tattttccga
    11161 aagggtacac ggatctaaga ttagaaggaa agcagtgcct ttctcttcat attccttccg
    11221 atgcagattt atctcagaat aagataagag atagttacga aagtgccatt cgtttttttg
    11281 aactttattt tcctgattac cattaccaag tgatgtacac agatacttgg ttactatcac
    11341 ccaatcttac taaatggttg aaaaaagagt caaaaatctg tctttttgca gccgattatc
    11401 gattattatc agttgatgaa caggacgata gtggagtccc ttggatcttt ggacgagtag
    11461 atgcccaaat acatgattat ccagaaagta cgagtttaca gcgacaagca aaagagcaat
    11521 tgcttgcagg cgagcacatt ggctctggct taggtattct tacttcataa gtttagtact
    11581 tcgaaatagt ttaaaagagc gtgagacaaa agtaaagaac acttttgtct cacgctctaa
    11641 acacgtataa acggcggaag cagaagcaac tccttcggaa ataagctgaa attcacaaaa
    11701 atttgaaaag caattttcgt gaatttcttc ttatttcttg gagttaaacg cttctgtccc
    11761 agcctctttt ttagtgttct atcgaacaca cagtacgttc aataggtaaa ctagctataa
    11821 caatagatat taaagttaac ggacaagagg gcctttttta ggtagctaaa acgtaataat
    11881 gaaaatttat ttccagagat aggagtcaat cagctagtct gatcgactgt tttaacaagt
    11941 tgactttcat tttaactttt gataccattt aaaacacaaa tcgtaattgt ttcgatttgt
    12001 gttttttagc atgactacaa tcaaagaaga aagtgggtcg tccatatgga aaaaacagat
    12061 atctcaagtg cataccgccg cttgaaaagc ccaaatatca aaacacgaaa acgtgcgctg
    12121 aagatcatca aagatgtgaa acggaatagt gggaaaaggt aagaagaagg aaacttaatt
    12181 aaaaaaatag ccgaaaaacg tttttcggct attttcaata atcggttttt gtgtaaggtt
    12241 tccgaaagaa ctgctgttga ttttccattt ttaacatatg ataaattggt agaatctttc
    12301 ttttcaaatt gataccattc cctaattcca gttatatagt taactacaga accacaaagc
    12361 acgtacgttt ataaaagtat tatctgtagt tatttttcaa ttccctttca acctcttatt
    12421 ttgaccagta gtattgatca ctttaccttt taacaccttt gttcgttttt gaaacatttt
    12481 tagtcaagaa tggaaacgta actacataat caaaggtttt gcgaaatatt tcatgaaata
    12541 tttcatgtag aagcgagcaa tacggttgca aataaccatt atcgtttaat aagcagttac
    12601 gaaagctccc aatatctcta acgttcagct cactccattt cttttcttca aaaaaatcag
    12661 tgattcgtat ctctttctat ttgtcgaaaa agttaacttt aaaaagtaac gccgaaaaaa
    12721 tatgcaaaaa aataaaaaag cagttactat tgctagaata acatcaagga ataataagtt
    12781 ttggttttca gataatagtc gataagttgt gtattggaat tttagataca gtaataaagt
    12841 atttttcatg aaattttgtg agaatcacta ttacaagtat ctttaataaa attaatggtt
    12901 aaggaggaac ttaaagtaga atgtaaatga atagttgtag tcagtcgatt gtgatactag
    12961 actcaaagtt gcaggaataa gtttttcaat ttgtgattgg aagataaaaa atgaaaaaga
    13021 agggcttttt tttatacttt gttgattgta gtgtttattt tccctctgtt tagccaagta
    13081 acattttcta tgactcaacc agtttcaagt gaggaacatg ataacctttt tcgtgctaca
    13141 gaagagttta agaaatatca ggaaaatatt gttccggaga ctccattagc tagtgagcca
    13201 attactgata ttgatggaaa ccaaatagga tggtatatac agtacgaagt tatggtggta
    13261 aataatgact caatagtaaa aaatacagta ggcttagagc taacaccgat tgaaaaggtg
    13321 agttcattgt taacgtatat ttatactgat gaaagtgacg agactgagat cattttaacg
    13381 gattactcaa aaattgctac tgataaaaag tatatattgt ggatgtagac actagtgaaa
    13441 tactttatga tatagaaatt gatgaaaatt tactagatgt agcacaagaa atcgatcatc
    13501 aaaagcagga agctattcga tatgctggta gaaaagggaa aagaaaatat ttattacgtg
    13561 aatgtagcaa aagttagaga agatgaaaat gagtggaaag aattcaagag tcgctactca
    13621 attaactcga ctccgacatt tacagtttac agagaaggat cgatagaaaa aactgttttt
    13681 tggactaaag aaagtggaat gtctttagct gaagtagaag agtttttgga ttatgttagt
    13741 atgcaacagt aagggagttc ttattatgaa aaattttgac acatattatg ttattgtttt
    13801 ttcgatatct ttgctgttaa tattactaac tttaatgcag tataaagagt atccagagta
    13861 ttttatttta acttttgtat atttagtaag tagctacaca cttctatata aattcagaag
    13921 ttcttctaga gtttctaaag tttgtttctt aattagctta ttgattctat aatatttttg
    13981 atttatatag gaaattaaaa atattataga atcaacactc agagaacggg ttggttttta
    14041 atagatttaa taagtactga taatgaaatt tctcctggtt gggaaaacga agttggttac
    14101 cctgtagaag aattttttca agattacttt gcggagtatg ccgataggat aatgagagat
    14161 tacgtaacag ataaaactca agaagtcatc aatcaatccc agtcattaaa tgaattatct
    14221 gataaggcga aagaagaagc agttgccatt atttcttcag ggattagtga ctatattgat
    14281 atgagtggtg aaatcaatta tggcgataaa gacaaaattg tacgcatgat aaaagctttt
    14341 atcaaaactt ttgatgtgaa ggcagatctt aagtttaatg atacttacct agtaggaaaa
    14401 ttaattaata tccttgggac agagaaagat acggaaacgt ttatttcctc tttgtctatg
    14461 ttgtttacag ataagcattt ttcaactcag cttggtggag aggaactagt ggaaatcttt
    14521 taaccattac gcccagcatt aataaaagta tatcaaaaaa gtactcctga tgatttttaa
    14581 gattggtttg agaaataatg tgaaaacact atttttaatg ttaattatag atagagatga
    14641 acgcgtcata tggttaaaag aatgaaaaag tcaaagatac actgctatat ttcactaagg
    14701 atatagcagt gtatctttca tgtcgtttca ctggcagttt tttataaaag gaggtgtgca
    14761 tttcagttgc ttttctttgg tagttagttg aatcgtttaa gaattaacgg atgtggcaga
    14821 caggtgctct gtccaaaaac cagtaaaatc tttgatggtt ccatctttat ttagctgtgc
    14881 ctgttcaaat actgttttgt ttttaccgaa tggatcaatt gcaataattg ttccgatccc
    14941 cgcacgtttg gcagcaagta atcctgagta agcatcttcg ataacaagac aatcagccgg
    15001 agctaagctc agtttgtctg ccgctttaag aaaaatatcc ggttgcggtt tcccagggaa
    15061 agagccgtcg tcatagacaa tcttgtccca gtcaaaccag cgggctaaat cgaaaatatc
    15121 aaaataaaaa gcaacattct ctttgacagt agccgtggca atcgttaatg gaatctgttg
    15181 gtcagtcagc tggtcaagag tcgcttcaag cccttttgtc aactgaagct cgttttcatt
    15241 ttgcaagcaa agcatacgat aatagctttc tttttcttct gacaatacgg cgatctcaga
    15301 ggcggtcaaa tgctctgaaa tgaaatgttt caaaatctcc gtattcgtcc gaccatgaat
    15361 attttttaaa atatcctcgt ctgataaacc atcttttgta tgatcatgta tcatatgcag
    15421 ccaagcagct tcatgcagat gagagtccaa aaacattgtt ccgttgaaat caaatataat
    15481 ccctttcata gttacgctcc tatagtggta ttttcggttg aatcagcaat ttgcagatcg
    15541 acatattgtg catagtgctc ttgaacctcc cgcgagatct ggcgatccgt aatgagcaga
    15601 tcgaaatcat gcaaattata aaacacataa aaatcatctc gattgaattt tttattatca
    15661 acgagtagat atttcttacg ggaattattt agagcgatct gctgtgcttc tccttcttcg
    15721 gtactgtaag tagagatcgc ttcgttgtga acgccatttg aactgataaa tgcttttgta
    15781 aactttagtc ttcttaaatt gtcgttggtg atcggtccga caaatgcgcc ggtatttttc
    15841 cggaaatcac cgccaatcag tagaatttct ggtccatctg cttgagcaag aatatcaaaa
    15901 actggataat tatttgtaat gatccgaatt ttcttgttca gtaaatgcgt cgccaatagt
    15961 tcgattgttg tacctggtcc taagaaaatc gtttctccct cgtgaatcaa ggaggctgct
    16021 gtggcagcaa tttctttttt ttcttgaatc tgcagagtgg ctttttcagt gtgagaaagt
    16081 tcttgatcta aagtgtagga aagactttga gcccctccat ggacacgcag cagcttgccc
    16141 gatttttcta attcatctaa atctctgcga acggtcatat cagaaacatc taattgattg
    16201 atgatttcat taacggtaag gattcctttt ttattgacca tttctacgat agtaagcaat
    16261 ctttcttttt tcaacattgt cttcacctct aatttaagga taatctatca tataaacgga
    16321 caacagtcaa tctaattcga acaaaagtaa acatttaggg tggtttttat gtttgttaca
    16381 atagatttta ttgctgtttt ctggttttta tgattatttg tgtttgtttt tgttgacaaa
    16441 tgttttggat gcgtttacaa tgcaatcagt tagaaagagc gaaaactgat tatagatttg
    16501 aggagagatg tacatgctta aattaccaga agattttatt tttggcggag ccacagcagc
    16561 ctatcaggtt gaaggagcca ctaaggaagg tggcaaaggc gctgttgctt gggatgactt
    16621 tttagaggaa caagggcgat tcagtcccga tccggcaagt gatttttatc atcaatatgc
    16681 aaaggacatt gaattgtgtg aacgttttgg tgtaaatggt ctaagattat ccattgcttg
    16741 gagccgcatt tttcctgatg gagctgggaa accaaatcca gaaggaatcg ctttttatca
    16801 tcgtgtgttc gaagaatgta agaaaagaaa cgtaacgccg tttgtgacgt tgcatcattt
    16861 tgatacccct aaacgtttgt ttgaccatgg agattttttg aatcgagaaa caatagaagc
    16921 atttgtttcc tatgccattt tttgttttca tgaattcaaa gaagtcaagg tgtggagtac
    16981 ctttaatgaa atctatccag ttgcaaccaa tcaatatttg ttaggtgttt tcccaccagg
    17041 aatcaaatat gattttacaa agatcgtcgc ctgtcttcat aatatgatgg ttgctcatgc
    17101 ccgtgtagtg aactatttca aagaaaacga acttccgggt gaaattggtg tagttcactc
    17161 attagagacg aaatatgcgg caacagacgc gccggaagat aagcatgctg cctttttaga
    17221 cgatgcattg tcgatccgtt tcttattaga tgctacctat ttaggctatt attcaactga
    17281 aaccttgact gctcttgatg agatatgtga agcaaatcag gccagctatc attttcctga
    17341 agaagatttc gttgaattga aaaaagcatc cacgcgtaat gactacttag ggatcaatca
    17401 ctaccaatgc cattttgtca aagcttacga tggagaaaat gcaatccatc ataacggtac
    17461 gggtgaaaaa gggacctctg tttataaagt gaagggaatc ggagaacgta tttataaaga
    17521 aggaattcct cgaactgatt gggactggct gatttatcca gaaggtctat atgacttgtt
    17581 gctgcgaatt aaaagcgatt acccacatta caacaaaatc tatatcactg agaacgggat
    17641 ggggtataaa gatcagtttg aagatggcat tattatggat cagccgagaa tcgactattt
    17701 gagagtctat ttggaatcct taagcaaggc gataacagcc ggggtaaatg tgaaggggta
    17761 ttttttgtgg tcgcttatgg atcttttctc atggacaaat gggtataaca aaaggtatgg
    17821 cttgttttat gttgattttg aaacccagaa gcgttaccca aaagaatcag cttactggta
    17881 caagttggtc agtgaaacca aaacgattat ttaataagtg ataatggagg aagaaaagat
    17941 gaataaagag gaattacaaa tgcttggttt tgagatcgtt gcatattcag gcgacgctcg
    18001 cagtacatta ttgaaattat tgaaagaagt aagacaagga aactttgaac atgttgattc
    18061 ggcgttgaaa gaagccgacg aaaatttaac ccttgcacat aattcgcaaa caaaaatttt
    18121 agcggaagaa gcatctggga aagagatgga gatgggattc atttttatcc atgggcagga
    18181 tcatttaatg accactttat tgctgagaga tctgattcaa gactttattg tactgtacag
    18241 acagaatcaa ggataggaga agaacgctat ggatggaatc attaaacaaa tcgaaaaagg
    18301 gaaaccattt tttgaaaaaa tctcacgtaa tagttattta ggagccgttc gtgacggatt
    18361 tttaacagcg atgccagcta ttttattttc aagcattttc attttgattg ctgcggttcc
    18421 tgagattttt ggttaccaat ggcctgaatc tgtctccaca tggctatgga aagtctataa
    18481 ttattcgatg ggtgttgtag ggttactcgt ttcagcaacc actgctcgct gcttggctga
    18541 ttcgatgaat cgtcgcttac cgaaaaacaa agtaatcaac accacctctg tgatgctggc
    18601 ctctgtggta agtttcctgt tgttaagcgt ctctccgatt gaaggtggat tttcaacgga
    18661 atatatgggg acaaagggac tactggcttc ctttgtcgct gcatttttaa cagttaattt
    18721 atataagttt tgtgtgctga aagatattac cattaaaatg cctaaagaag ttcctggaac
    18781 gatttcgcaa accttccgag atatttttcc tttctctttt tcagtatttg cggccgtttt
    18841 aattgattta ggcattcgac aaatcttcca aatctcattt gcagaagcaa tcattacggt
    18901 gctgcagccg ctatttacag cagctgacgg gtacttagga atcgctatta tctggggagc
    18961 gatggcgctg ttttggtttg tcggtgttca tggaccgtcg atcgtggagc cggcaattgc
    19021 ggcaattatc tatgccaatg tggaaaccaa tctgcaattg ttcaattccg gtgaacaagc
    19081 ttctaaagta ctgacggtgg gtttagggaa ttttgtagga actatgggag gaactggggc
    19141 aacattggtt gttcctttta tcttcctact ctttgctaag tctaagcagt tgaaggcagt
    19201 tgggaaagct tcctttattc cagtaagttt cgctgtcaac gaaccattgc tatttgcggc
    19261 acccatgatt ttgaaccctt atttttttat tccatttttg ttaacacctg ttgtaaacgt
    19321 ttgcctgttc aagttttttg tcgatgtatt gggcatgaac agctttatgt acgttttgcc
    19381 ttgggcaact ccagcaccga tcggtttggt tctaggaacg ggcatgggta tcttagcttt
    19441 tgttctagca gcggttctaa tcgtcgttga cttcctgatc tatctaccat tttgcaaagc
    19501 atacgatgct gtcttagtag agaaagaagc acaaaaagca gaggtgatcg caaccgagga
    19561 aggtgcttct gccgatgtac gtgaggaagt acctgtggca gcgagtcaag ttatcagcga
    19621 tgcaaataaa gaaatcaatg tgttggtact ttgtgctggc gcagggacaa gtgcgatgtt
    19681 agctaatgct ttaaaaaaag gtgcatctga atcgaaagca ccgatcagtg caatggcggg
    19741 tgcttatggt tctcattacg aaatgctgca ggattttgat atggtggtct tagcaccgca
    19801 agtcaattct tactatgatg atatcaaaga agacaccgat cgcttaggaa tcaagctggt
    19861 tgcgacaaaa ggagcagaat atatcggatt aacgagagat cctgaaaaag ctgttcagtt
    19921 tattctgaac caatttcaat aaaggaggag ccataatgaa aatgggaatt tggttgcgta
    19981 aagctgtttt atttgcagct gtgttagggg ctttgggggc tgtttcaaaa actgttgaag
    20041 catctgccag cgaatttatt cgcggagcag atatttcgat cctgtcagat atggaaaaat
    20101 ctggtgcaaa atattatgaa gacgatattt caaaagatgc cttagagatt ttaaaaaata
    20161 atggtgtcaa ctatgttcga ctgcgtttgt ggcaggatcc ttataatagt aatggagaaa
    20221 gttatggagc agggacaaat gatctggaaa cgacgattga ccttgcaaaa cgagcaaaaa
    20281 atctgggact taaggtgctg ctggattttc actacagtga tttttgggtt gatcctggga
    20341 aacagaatct tcctaaagca tggcaaggtc tgacctttga agaaatgaat acggcattgt
    20401 acgactatac aaagaacgtt ctgtcggaaa tgaagcagct ggacgtttat cctgatatgg
    20461 ttcagatcgg caatgaactg aatagcggaa tgctctggcc ttatggcaag tcttggggag
    20521 aaggcggggg agaatttgat cgattggctg cttttttgaa gtctggtatc caagctgttc
    20581 gagacactca acctaaaaca actccagtaa tgctccactt agctgatggt ggcgatacag
    20641 gagcatttac ttggtggttt gatgaaatca cgagtcgagg tgtgtcattt gatttaattg
    20701 gagtgtcgta ttatccgtat tggcacggtt caatgaatga acttcaaacc aatatgaatg
    20761 cgatcagcca acggtatggt aaagaagtga tcgtagttga aacagcttat gggcatacaa
    20821 cagcgaatgc cgatactatg ccaaatgctt ttggtgaagc cgaagcagct gcggggggat
    20881 atgaaccaac accgacagga caagcagagt atttgctgga tcttgccgat cggattcagg
    20941 cagtaccgaa taatcgcggg gcgggcttct tctattggga acctctttgg tacaacggaa
    21001 atgtgagttg ggcaacacaa gcaggtatga attatctcag tgttcaatcc actatgggaa
    21061 acgaatggga caaccaagcg atgtttgatt ttgctggaaa cgccttgcca agcttgcgtg
    21121 cgtttaaaca agcaggggcg caaataaatc tagttaaaaa tgcaagtttc gaaagcgatg
    21181 gaccaacgac cagtccttcc aattggcaaa cctggtttca acaaggaacc gctaagggta
    21241 cgatcaaaac ggaagcgggc actttttttg gggactacaa actaactttt tggcaggatc
    21301 aagcatacga ggcatcagct tatcaaaatg ttagtggcat aagcaaagga acttacacat
    21361 tgagtgccta tgtaatgtct gggggagagt ttgatactaa tcagttatat atcaaaaatt
    21421 atggtggagc ggaactaaat actgcggttg ttaaaacaag tgaatggaca aaaattacaa
    21481 tcaaagacat tccaattaca acaggtgttt gtgaaattgg ggtttatagc aaggcttcag
    21541 gaaatgcttg gacgaacgtt gattttgttt cattggtgaa acaatagact gcggatcgtt
    21601 tcgattcaag atcggaggtt cccttcagcc acatcgttag cgtagaaggc aggaatcgaa
    21661 cctgcacctc gcctttgcca aaaataacca gaaacgattt ccagtcattt ttaccaaaac
    21721 gagtaaagca ccagacttca tctacacttt gtattatgct aaagagagtt caacgatttc
    21781 gtaagaagca atgaaaaaat tgcaaaaagt tgtgcagata acataaagtt tgtttaagaa
    21841 agggtaacct tagaattccg tcgtgaatat gatttgagca cttagatgga tagaaaaaag
    21901 ttctcggaaa acagttcgtt tttcgagaac ttctttttat gataagaatg aaacaaatta
    21961 acttatatta tagtctcttt tggtagggaa ccatcttatt tttaatgaaa atcaagcatt
    22021 ttagctattg aagaatgttg atttataggg attccccctc catattatgt tggctcttta
    22081 ttcccactct gttttcaacg taaagtgtat aaaacagaat gcgatactat caagggagtt
    22141 aaaacaatat acagaacagt aaaacactat aaataaaaat aaaagtgcaa taaaagtgca
    22201 atttttgcac ctaatcatac tctgtttata tatgaaaact agattctgaa tccaatattt
    22261 ggttaacgag aggcgttcga atttgaacgt cttttatata ttactttcaa ataaatatga
    22321 ggtagaatac tatctatttt ctttttaacc caattgttat aatggagagt gtaaaatatt
    22381 ttgtgtaaat agaaaaaagg aagtcccttc tgtagaatag agttaccaca acacattcac
    22441 agaagagagg acttccctat gaacgatttt actacagaaa ttctaaagac tctagcgaac
    22501 aaaggcgatt tgaatgaatt attccgtgtc catttggaga aagcagtcaa tacgcttctc
    22561 aaaacggagt taacggcttt cctagattac gaaaagtatg atcgcattgg ttttaacacg
    22621 ggtaattctc gtaacggctc ctatgaccgt acggtcaaga ccgagtatgg ggaacttcat
    22681 ctccagattc cgcgcgaccg caatggtgag ttcaagcaac agactgttcc tgcttataga
    22741 cggacgaatg acacgttaga ggagaccgtc attcacctct tccgaaaagg tattaccatg
    22801 tcggaaatcg cagacttgat tgagaaaatg tatgggcatt actacacgcc ccaaaccatg
    22861 tccaatataa caaaatcatt tacagaagag gtaacggcgt ttaatgggcg ggagcttcat
    22921 gaccgttatg ctgctattta tatggacgca acgtatattc cgttaaagcg gaaaaccgtc
    22981 gccaaggaag ctattcatat cgcagttggc attcgcccgg acggatcaaa ggaagtattg
    23041 agctatgcga ttgcac
//
LOCUS       DACEEZ010000040        22990 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20313_2017
            SAMN12639966-rid9879223.denovo.040, whole genome shotgun sequence.
ACCESSION   DACEEZ010000040 DACEEZ010000000
VERSION     DACEEZ010000040.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639966
            Sequence Read Archive: SRR10023158
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 22990)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22990)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741683.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741683.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:44:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,016
            CDSs (total)                      :: 2,962
            Genes (coding)                    :: 2,889
            CDSs (with protein)               :: 2,889
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 36 of 73
            Pseudo Genes (incomplete)         :: 29 of 73
            Pseudo Genes (internal stop)      :: 18 of 73
            Pseudo Genes (multiple problems)  :: 8 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22990
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639966-rid9879223.denovo.040"
                     /isolate="E20313_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(109..564)
                     /locus_tag="IWO89_001571"
     CDS             complement(109..564)
                     /locus_tag="IWO89_001571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0456319.1"
                     /translation="MSKEMTALKFYFRNGETWTINRRHIGDLWIKQITTSFGRINGSE
                     FVEIHPCAGFKIEIFHEGDAVATHDINLGGLEMGMFNRALKYEDIERMEILYRNGTPD
                     LVYFPYLDKGTEGLDNQYQSTKISEKTGNLYIVINPDQRVEDVYGEFFE"
     gene            complement(718..1926)
                     /locus_tag="IWO89_001572"
     CDS             complement(718..1926)
                     /locus_tag="IWO89_001572"
                     /EC_number="2.7.7.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CCA tRNA nucleotidyltransferase"
                     /protein_id="HAQ0456320.1"
                     /translation="MKLKQLPLEYQKAIPVLKKLENAGYEAYFVGGSVRDILLNQPIH
                     DVDIATSAFPEEIKQLFPKTIDIGIEHGTVLVLENDEQYEITTFRTESTYQDYRRPDH
                     VEFVRSLAEDLKRRDFTINAFALKEDGEIIDLFDGLQDLEEQILRAVGNPHERFHEDA
                     LRMMRGLRFVSQLGFKLEEETFYAIKENHELLGKISVERINVEFIKMLLGNEGAAGIH
                     AFIETECYICCPGLKEEGEALLRFAELPSVPLKEESQAWALLISQLDLKENEIRSFLK
                     SWKCSNQMIKDVQALVQGLRQRETGALEPFELYFLGKKHALQVEELMIYFDETPQMDA
                     VGESYDRLPIKSLSELAVDGKILLEETGKKPGKWVSEALQLAEKAVVEQRIKNDKKQV
                     LDFLSKEKLI"
     gene            complement(2038..2370)
                     /locus_tag="IWO89_001573"
     CDS             complement(2038..2370)
                     /locus_tag="IWO89_001573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide pyrophosphohydrolase"
                     /protein_id="HAQ0456321.1"
                     /translation="MDKKRSLYSMQQEVDEYIQQFKVGYFSPLAQMARLTEEVGELAR
                     EVNHTYGEKSKKASEPVNSVAEELGDVLFVTMIMANSLNIDLTEVFEKNMEKFNQRDH
                     NRFERKDE"
     gene            2518..3393
                     /locus_tag="IWO89_001574"
     CDS             2518..3393
                     /locus_tag="IWO89_001574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HAQ0456322.1"
                     /translation="MVKRKTVKEVLFIIIGTSIYAFGLVYLNIANHLAEGGVSGITLI
                     LRALFGIDPAYTTLLINIPLILLGGKILGKRSLAYTVLGTLSLSFFLWIWQRVPLEIN
                     LQHDLLIVSLLAGLVAGVGSGIVYRMGGTTGGSDIIARILEQNYGITMGRSLLAFDII
                     VLLASLTYIDLKRMMYTLIVSYVFSRVIDSILDGGYSAKGLLVVSNKSEEIAPLLMTG
                     LQRGVTFLSGEGAFSGNSKKIIYMVVSSSELTEAKRIVHEIDEKAFLSIINVHEVEGQ
                     GFTYLKPQTRLLKKL"
     gene            complement(3475..4056)
                     /locus_tag="IWO89_001575"
     CDS             complement(3475..4056)
                     /locus_tag="IWO89_001575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IDEAL domain-containing protein"
                     /protein_id="HAQ0456323.1"
                     /translation="MLIDVREKKNFLTWMVNHVSFSRREVFWILNYLANHEAILSNVH
                     FVEGANFADRGLQISDLSVEGEPMKLFLQGKEFTDTDQIFHEIRLNWKKPLYVECIFE
                     NAWQTREYLSILEDNPFSSWDSSVSEEDTELIERYFQEKEEEAKLKMLYAQIDQALED
                     GDRDAFLKLSDEVNRKILLQTQKKSNRVSEHNN"
     gene            complement(4062..5321)
                     /locus_tag="IWO89_001576"
     CDS             complement(4062..5321)
                     /locus_tag="IWO89_001576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HAQ0456324.1"
                     /translation="MNTNSEKMLQALSDEDLSQAQIFLEKALKEDPADILAELGEELL
                     AIGFLIEAKQIFEQLINQYPENDGLNIPLAEIAIEDNEIDLAFEYLEKVDKQSDYYPQ
                     SLLAIADLYQVIGIPEVSEAKLKEAAGLLPDEPLIQFALAELYFSVDRFKEAAVVYET
                     LLASGINEISGISMQERLGQSLSMQGEFEAAIVPLEEALKEERTDDRLFQLAFTNLQL
                     KENQQAINYLEELREVNPHYQSLYLYLGQALQEEEMIEEAQTVLEEGIKENPYQVELY
                     HLASENAFRLHDRQRAEELLLKALEVGDKQDETLLTLSNLYLDDERYEDVIQVINQME
                     ETANPYAEWNLAHAYNELEDFTVAAVHYEQAYHELSHEPDFLKEYAFFLREEGQLKRA
                     QELLTYYLTLEPGDMEALSLLDDLTER"
     gene            complement(5323..6369)
                     /locus_tag="IWO89_001577"
     CDS             complement(5323..6369)
                     /locus_tag="IWO89_001577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0456325.1"
                     /translation="MELDYQVEDALTKVFYGETLVSQLRELSIDQPILFLTNQRYYDL
                     FADKINPLFANQANNDWYICANTQCNHLTELKNLLDFVKRYPENQSMFIIGFGNEGIM
                     DLAGFFQKHTTLDSSLWMIPVSIRSLSRALTPQRTILQLPNRVVLQTINLPERIVYDQ
                     TISERQVDGKQIDFLTFICCGMICDHRFLQNLYKNYPNKKQLTTRPFAGMIEEMIYFY
                     QLEAENLSNYGKLFEQAFYLTENGHLLSASMKKFLGMMFHFVWNLQAYPFDFQLKNFF
                     VWLDLLGYPIHWLEQISKMEYLEKVLFLAEKSKKILVLEKIGIIDGYQHPNEKELLRT
                     MTVFQQIVSEIRGN"
     gene            complement(6604..6879)
                     /locus_tag="IWO89_001578"
     CDS             complement(6604..6879)
                     /locus_tag="IWO89_001578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008214712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HAQ0456326.1"
                     /translation="MANKAELIEKVASATDLTKKDATAAVDAVFSTIQDALANGEKVQ
                     LIGFGNFEVRERAARKGRNPQTGEEIEIPASKVPAFKPGKALKDAVK"
     gene            complement(7122..8432)
                     /locus_tag="IWO89_001579"
     CDS             complement(7122..8432)
                     /locus_tag="IWO89_001579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HAQ0456327.1"
                     /translation="MANPTIAIVGRPNVGKSTIFNRIAGERISIVEDTPGVTRDRIYA
                     KGEWLGREFSVIDTGGIDLGDEPFMDQIKHQAEIAIEEADVIICVVSGREGVTDADEM
                     VAKILYRSNKPVILAVNKVDNPEMRNDIYEFYSLGLGDPYPVSGSHGLGIGDILDEAV
                     KYFSEETEEEDDDTIKFSLIGRPNVGKSSLINAILGEERVIVSDIEGTTRDAIDTHFV
                     SESGQKFLMIDTAGMRKRGKVYENTEKYSVMRAMRAIDRSDIVLMVLNAEEGIREQDK
                     RVAGYAHEAGRGIIIVVNKWDLVKKETNTMRDFEQEIRDEFRYLDYAPIVFVSAVTKQ
                     RLERLPEMIEQVSMNQNLRISSAVLNDIIMDAVAINPTPTDKGKRLKIFYATQVAVKP
                     PTFVVFVNEEELMHFSYERFLENQIRKAFTFEGTSIRIIPRRRK"
     gene            complement(8614..9843)
                     /gene="rpsA"
                     /locus_tag="IWO89_001580"
     CDS             complement(8614..9843)
                     /gene="rpsA"
                     /locus_tag="IWO89_001580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S1"
                     /protein_id="HAQ0456328.1"
                     /translation="MTEFDQNQENNQSMEDAMKSVQEVKVGDVVKGEVLVIEDKQVIV
                     GIEGAGVEGVVPAKELSTLPVEDISEVVKVGDVLDLVVISTIGKDKENGSYLLSKRRL
                     DAKKVWEDIERDFQEGKVIEAPVTNVVKGGLVVDVGVRGFVPASMVEDHFVADFSDYK
                     GKTLSFKIIEIEPSENRLILSHKAVVENEREEKKKEILSTIHEGDVLSGRVARLTDFG
                     AFVDLGGIDGLVHVSEISHSHVAKPSDVLAVNDDVNVKVLSINPEQERVSLSIKDTQP
                     GPWSEIDTKAPVGTVLDGTVKRLTSFGAFVEVFPGVEGLVHISQISHKHIATPHEVLH
                     EGDEVKVKVLEIHPEEHRIALSIKALEEKPASEEEPKNVESYELPEENTGFTMGDILG
                     DALKEEVEEKATDDSEK"
     gene            complement(9978..10658)
                     /locus_tag="IWO89_001581"
     CDS             complement(9978..10658)
                     /locus_tag="IWO89_001581"
                     /EC_number="2.7.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HAQ0456329.1"
                     /translation="MTQISIAIDGPASSGKSTVAKILAKELNYIYTDTGAMYRAVTYL
                     AITNNVSFSDEEGLVSLIKKYPISFSQQEDGQHVFIASKDVTLEIRQPDVTKAVSEVS
                     AHGKVREELVAVQRKIGERGGVVMDGRDIGTAVLPNAEVKIFLVASVSERAERRFKEN
                     QEKGIPTDLETLTKEIQERDHYDSTRDVSPLKQAEDAVRIDTTGKSIPEVVAAIKAVV
                     LKKGYLLF"
     gene            complement(10707..11342)
                     /locus_tag="IWO89_001582"
     CDS             complement(10707..11342)
                     /locus_tag="IWO89_001582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HAQ0456330.1"
                     /translation="MSRKDRHQSTENNETQEPWEQPIYDTDDEVSSRSSQRQQKKGNT
                     LFLSLFLILLVLCIAIPAGAYFWIRNGSSNNATAATSSETSTSVVESSTSTTKEESTE
                     TSTVESSTIAEESTVSQDTGVTSESSIAAETTPTSSSEATYQAGDSSSNAAETTPSSS
                     AAAGSTTTVQAGEGPKQVAERAGITTDQLFELNGLDPNNFMLYPGQELRIK"
     gene            complement(11408..12811)
                     /locus_tag="IWO89_001583"
     CDS             complement(11408..12811)
                     /locus_tag="IWO89_001583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecQ"
                     /protein_id="HAQ0456331.1"
                     /translation="MLINELKKYFGFDSFRPGQEEIIQALLDGEDTLAILPTGTGKSL
                     CYQLTGYLMEGLVIIVSPLLSLMEDQVTQLQKRGEKRVAAFNSLLSQSERRYVLRHLS
                     QYKFLFLSPEMLTNPSLLEHLKKQNIALYVVDEAHCVSQWGVDFRPEYQQLGKIRKLL
                     GNPVTLALTATATDLVARDIRHVLFDRPPKEIRQSVNRQNISLFVRKTQQKEQELEHF
                     MERAQGAAIIYCATKKEVERLYHLFRERFTVGYYHGGLDAAQRRQLQQQFVKNQLQFL
                     IATNAFGMGIDKSDIRYVVHYDLPDSLENYVQEIGRAGRDQEESAAILFYQSGDERIH
                     YFFNQLSREQRQSFELYLEYATKQAPFDELQKKWMELIQQSEKPENWVERLKRQEKEK
                     EFRLQQMLRYINEENCRRKFILAYFGEKLSEKPQNCCDIDGAKAELLPKRKLFSKTEQ
                     LHWETILLNLFKKNMEH"
     gene            complement(12798..13829)
                     /locus_tag="IWO89_001584"
     CDS             complement(12798..13829)
                     /locus_tag="IWO89_001584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0456332.1"
                     /translation="MIVFILSLFSSGHKLRISSLYQLLVGKRTTSVLIYGFTHELLFI
                     HNSFPELKQDKFYQILQKIAQQGWIEINENEAKLTPAGADMLSEHQIEHTGLRFDRYG
                     RTGETSWRLIKFAVQVISNLATGIQDYLPAETSPFYTFQLKKWLSESQLPREILIDTA
                     YESLVQLFSEIPEEAADFLANQLSGNERTGLLPYQLAKNNDESEVYLQQSRCIHLLLA
                     QIEKRPDSLWHALIDSLLQQNFNQSMMITKQMFMNGQTIDQIMAIRHLKRGTVTDHLI
                     EWALFFDDFPYEWILSAETIERLEPNKDSVREWRFSEWNVDGQLDYGEFRLYQIYLLR
                     KEAIQNVNK"
     gene            13860..14081
                     /locus_tag="IWO89_001585"
     CDS             13860..14081
                     /locus_tag="IWO89_001585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin"
                     /protein_id="HAQ0456333.1"
                     /translation="MSLLCKLVPERCIACGLCQIEAPDIFDYDEEGIVLFAQEPEAHQ
                     QFVMPEEETYVKKAYQKCPVRAILLENKI"
     gene            complement(14246..14983)
                     /locus_tag="IWO89_001586"
     CDS             complement(14246..14983)
                     /locus_tag="IWO89_001586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase"
                     /protein_id="HAQ0456334.1"
                     /translation="MKIFRRILIGLVIILGLLGGAGYYYIQQNTYEPSSEAQVLVKEG
                     KNKEDYLFFEGEKKNHGKSPLLIFYPGALVEPESYSIWAQDLAKNGFSVAIVKMPLDL
                     AVMGGNKADKVKEDFPNSDYVIGGHSLGGVMASRYVNKNHSDKHLKGVFFMASYPDKK
                     GTLTESAVPVLSITATKDKVLNQEAYQEAKQYLPERTEYVSINGGNHGGFGSYGEQKG
                     DGEAEISNADQQTVIVNTLENWLKKLK"
     gene            complement(15136..16476)
                     /locus_tag="IWO89_001587"
     CDS             complement(15136..16476)
                     /locus_tag="IWO89_001587"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Sapep family Mn(2+)-dependent dipeptidase"
                     /protein_id="HAQ0456335.1"
                     /translation="MKKFVTEQHHEQALESLSELIRIPSVLDEADSGQGHPFGKKVIE
                     ALDKVLEISEKLGFRTFKDPEGYYGYSEIGSGDELFGILCHMDVVPAGDENNWETKPF
                     DPTIKDGWLVGRGSQDDKGPSIAAMYAVKALIDAGVEFKTRIRFIFGTDEENLWRCLE
                     KYNEKEEGITQGFAPDAEFPLIYAEKGLLQAYLTGPGTNEFSVKAGGALNVVPDAAPY
                     SGEKLSEVKEALKKHEFDFEEQGEGIVVLGKSIHAKDAAQGVNAISRLAIALSEVFDF
                     GPINFLGKLVQENATGEAVVGKTEDEQSGELTMNFASLEITPEQTKIGVDMRIPVTFK
                     KDDLVAKLTKTAEKYGLTYEEFDFLDSLYVPLDSELVKNLLGTYRDITGDMTEPFVSG
                     GATFARTMNQCVAFGAMFPDTPDFMHQANERWELSSMYKAMEIYAEAVYRLCAK"
     gene            complement(16713..18236)
                     /locus_tag="IWO89_001588"
     CDS             complement(16713..18236)
                     /locus_tag="IWO89_001588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfcC family protein"
                     /protein_id="HAQ0456336.1"
                     /translation="MEQTKKKKFQMPSAYTVLFIIIAVIAVLTWFIPAGHYSVDDAGN
                     IVAGSYERVQQNPQGLWDIFMAPINGMLGVPAGELTKETPAAIPISFFILVVGGFLGI
                     INKTGALDAGIASVVKKFKGKEKMLIPVLMLLFALGGSTYGMAEETIPFYALLIPVMM
                     AVGFDTVVAVAIVLVGSQVGCLASTVNPFATGVASQAVGISMGEGIGLRFLMFVILLA
                     ISIVYVYRYASKIEKDPTKSLVYNQRKEDMKHFDIEAIGRQEVITKKQKHVNVLFFIT
                     FGIMIISLIPWTTLNANFTIFESLTNWLTNLPVLGTVLGKSMLPLGDWYFPEITMLFL
                     VMSIVVAITYGMKESEFVSEFLAGASDLIGVAIVVAVARGIQVVMNNGLITDTILHWG
                     EQGLSSLSSSVFIIITFIFYIPMSFLIPSTSGLAAATMGIMGPMGEFAGVGKDLVITA
                     YQSASGLVNLITPTSAIVMGAVAIARFDVSVWWKFTGKLIAILFVAICVILGVAAMF"
     gene            complement(18614..19225)
                     /locus_tag="IWO89_001589"
     CDS             complement(18614..19225)
                     /locus_tag="IWO89_001589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HAQ0456337.1"
                     /translation="MKNTRVQKMVAVAMFAAIALVLQYFAFPIMPAFSFLKIDFSDIS
                     VLISMFLFGPAAGVATAFLRSVLHLLTTGFSPDNLVGDTASFLSTTIFTFPIYYFFSV
                     KKQHKGRNKFLGVITGTIALTIFMSIANYFVITPLYLMFLGLNATQMLGMSLANYVLI
                     GIVPFNLIKGFIVSAVFLVLHAQLLPWLSRKQHQFEQRHTAVK"
     regulatory      complement(19348..19473)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00050"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="FMN riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="flavin mononucleotide"
                     /db_xref="RFAM:RF00050"
     gene            complement(19555..20271)
                     /locus_tag="IWO89_001590"
     CDS             complement(19555..20271)
                     /locus_tag="IWO89_001590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA pseudouridine synthase"
                     /protein_id="HAQ0456338.1"
                     /translation="MERLQKVIAHAGITSRRKAEEFILAGRVKVNGQVVRELGIKVGK
                     HDTVEVDEVPIYQEEYGYYLLYKPKGVISAVADDKGRKVVTDLLPMVKERIYPVGRLD
                     YDTSGILLLTNDGDFAQRLTHPKHEVDKVYVAKVKGIATKSMLAPLTKGIKIEGRKTS
                     PAKFQILSVDPKTNHSIVELTIHEGRNHQVKNMLQAVGLPVQKLKREKYGDLTLQGLR
                     PGDYRELNKKEVSSLLNHSK"
     gene            complement(20277..20846)
                     /gene="scpB"
                     /locus_tag="IWO89_001591"
     CDS             complement(20277..20846)
                     /gene="scpB"
                     /locus_tag="IWO89_001591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SMC-Scp complex subunit ScpB"
                     /protein_id="HAQ0456339.1"
                     /translation="MTKLSELEALLFIVGEEGISNEELSGLLNLSEAEVTSLVKELKE
                     SYTQNSHSALHIFETESHYVLTTKKELAPLLKRYAQGTSNTLSQAAVETLAIIAYKQP
                     ITRAEIEQIRGVQVSGAVQRLAAHKLIEEKGRVEGPGRPILYGTTGYFLDYFGLKSLE
                     ELPDTQEMEISLEEDVSPDLFFDGMNKGE"
     gene            complement(20830..21627)
                     /locus_tag="IWO89_001592"
     CDS             complement(20830..21627)
                     /locus_tag="IWO89_001592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="segregation/condensation protein A"
                     /protein_id="HAQ0456340.1"
                     /translation="MASKEKEERVNELTEINIKLDVFEGPLDLLLHLIQQMEIDIYDI
                     PIATITEQYMNYIHTMKTLELAVAGEYLVMAATLMAIKSKTLLPVQEFGTDDEELWEE
                     DPREELVTQLLEYRKYKYAAERLTEKAEERSLYYTKEPMNIDGLADAEKPLKRGQVKK
                     IDLIRAMQKILERKKFSQKVEKTITPDDTTIEERMVFIEERIKRNAHGCTFDSFFESP
                     SKSEVVTTFMALLELMKKGQIIAQQKENYESIMLYPAVGDTEHDEIK"
     gene            complement(21597..21968)
                     /locus_tag="IWO89_001593"
     CDS             complement(21597..21968)
                     /locus_tag="IWO89_001593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HAQ0456341.1"
                     /translation="MLTHYRKENRKIAMGLLSFHKKLTGKTSLLKEIDTYESEDDYEL
                     FFFTPEGSTNIQGIIGIYWATNDQLIIHDISLNPSYRGENMGFQMLDELQEKYPDVSI
                     RATEITEPYIGKWLAKKKKKE"
     gene            complement(21987..22874)
                     /gene="xerD"
                     /locus_tag="IWO89_001594"
     CDS             complement(21987..22874)
                     /gene="xerD"
                     /locus_tag="IWO89_001594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific tyrosine recombinase XerD"
                     /protein_id="HAQ0456342.1"
                     /translation="MKEQINDYLHYLNIERGLSANTRQSYERDLEQYLHFLQEQKIAS
                     WQDVDRFLVIRFLEKLHEEKKATATVTRMISSLRRFHQFLRQERITDHDPMQHIDTPK
                     KAQKLPSTLTLTEVEKLIEAPDTTKTLGIRDRAILEVMYATGMRVSELIGLKLGDLHL
                     SLGLVQTVGKGDKERIIPLGDYAIQWLERYLDEARPLLVKDASEMHVFVNNHGKGLSR
                     QGIWKNLKQLVREAGIYKEVTPHTLRHSFATHLLENGADLRTVQELLGHADISTTQIY
                     THITKKRMTDVYKQHFPRA"
BASE COUNT         6446 a         4914 c         3898 g         7732 t
ORIGIN      
        1 aaatcttgtc acagtccctt attctgaata aacggtgtgg aagagacagc ctatcagtag
       61 taataagaca catctctttc acagcctcat ttttgcgtaa aaatcgattt attcgaaaaa
      121 ttccccgtat acatcttcta cacgttgatc tggattgatc acgatataca agttacctgt
      181 tttttcgctg atttttgttg attgatattg attgtctaat ccttctgttc ctttatctag
      241 atatgggaag tacacaagat caggtgtgcc atttcggtaa aggatttcca ttcgctcgat
      301 atcttcgtat ttcaatgcgc gattaaacat gcccatttcc agtccgccta ggttgatatc
      361 atgtgtcgca actgcgtccc cttcatggaa gatttcgatt ttaaatccag cacacggatg
      421 gatttcaaca aattcactgc cattgattcg tccaaaactt gttgtgattt gtttgatcca
      481 aagatcgccg atatgacggc ggttgatcgt ccaggtttct ccatttctaa aataaaattt
      541 tagcgctgtc atttctttac tcattaaaaa gactccttta tgatgtaatt tattcttgta
      601 ctacctcagt ttagcacaaa tttatgtaag tggtcacatc ttatgttttt tcttctaatg
      661 acgtatctgc ccactttaag tagccaacaa agctgagcag atacagtcta gtgtttttta
      721 aatcaatttt tctttcgata aaaaatccaa aacctgtttt ttatcatttt ttattctttg
      781 ttctacgacg gctttttcag ctaattgcaa agcttcacta acccacttac caggtttttt
      841 tccagtttct tctagcaaga ttttaccatc tactgctaat tcgcttaatg acttgatagg
      901 taaacgatca taggattctc ctacggcatc catctgaggt gtttcgtcaa aatagatcat
      961 caattcttct acttgcagtg catgtttttt gcctaagaag tacaattcga atggttccaa
     1021 agctccagtt tctcgttgtc gtagtccttg gacaagtgct tgaacgtctt taatcatttg
     1081 gttagaacac ttccacgatt tcaaaaagga acgaatctca ttttctttta gatccagttg
     1141 gctgatcaat aatgcccatg cttggctctc ttctttcaat gggacggatg gtaattcagc
     1201 aaaacgtaaa agtgcttcgc cttcttcttt caaaccggga cagcaaatgt agcactctgt
     1261 ttcaataaat gcatggattc ccgcggcccc ttcatttccc aagagcatct tgatgaattc
     1321 cacatttata cgttctacag atattttacc taatagctcg tggttttctt tgatggcgta
     1381 gaaggtttct tcctctaatt tgaaccctag ttgactgaca aaacgcaacc cgcgcatcat
     1441 tcgtaaggca tcttcatgga accgctcatg aggattgcct acagctcgca agatctgttc
     1501 ttctagatct tgcagaccat cgaacaaatc gatgatttct ccatcttctt tcaaagcaaa
     1561 cgcattgatc gtaaaatctc ttcttttcaa atcttctgcc aacgaacgaa cgaattcgac
     1621 atgatctggt ctacggtagt cctgataagt tgattccgtc cgaaatgtgg tgatctcata
     1681 ttgttcatca ttttctaata ccaacacagt tccatgttcg atcccaatat ctatcgtttt
     1741 tggaaaaagc tgtttgattt cttctggaaa tgcgcttgtt gcgatatcca catcatggat
     1801 tggctggttc agcaagatat cacgaacact tccacctaca aaataagctt catatcctgc
     1861 attctctaat tttttcagaa ctggaattgc cttttggtat tccagaggta attgttttaa
     1921 tttcattttt ccaacctttc ctgcgtgttt cttgtcctga gataaagact cagttgcatg
     1981 gtgactgttt gacaagtccg tttcagagtc ttcaaggcca ggaccacttc atcttgttta
     2041 ctcgtcctta cgctcaaagc gattatggtc tctttgattg aatttttcca tgtttttttc
     2101 aaaaacttca gttaagtcga tattcaatga attagccatg atcatcgtaa cgaataaaac
     2161 atcaccaagt tcttctgcta ccgagttaac tggttcagaa gcctttttgc ttttttctcc
     2221 ataagtgtgg ttgacttctc ttgccaattc acctacttct tcagttaatc gagccatctg
     2281 agctaatgga gagaaatacc ccaccttgaa ttgctggata tattcatcca cttcctgctg
     2341 catactgtat aaagaacgct ttttatccat ctttttccct ccaaaactat cttaccaaaa
     2401 ctttaacgca aatcctagat aagagcaatt gagaaaatcg aatttccatg ctacatttag
     2461 gaaggaattt ctaaatggaa attctgtgaa aatacataat tgaaagctgg aattcatatg
     2521 gtcaaacgaa aaacagtaaa agaagttctt tttatcatta taggaacaag tatttatgca
     2581 tttggtcttg tttacttgaa catcgctaat catttagcag aagggggagt ctcagggatc
     2641 accttgatct taagagcttt atttggtatt gatccagctt atacgacttt attaatcaac
     2701 atcccattga ttttactagg aggaaagatc ctcggtaaac gttccttggc ctatactgtt
     2761 ctaggtacac tttctttgtc attcttttta tggatctggc aaagggttcc actagaaatc
     2821 aatttacagc atgatctact gatcgtttct ctacttgcgg gtttagttgc aggtgtagga
     2881 agcgggatcg tctaccgcat gggtggaaca acaggcggca gtgatattat cgctcggatt
     2941 ttagaacaaa attacggcat cactatgggg cgttccctat tggcatttga tatcatcgtc
     3001 ttacttgctt cattaaccta tatcgacttg aaacgtatga tgtatacgtt gattgtttcg
     3061 tatgttttca gtcgtgtgat tgattcgatc ttagatggcg gatattctgc aaaaggtcta
     3121 cttgtcgtct ccaacaaaag cgaagagatt gcaccactac tgatgaccgg tctgcaaaga
     3181 ggtgtgactt tcctttctgg agaaggcgca ttctctggta attcaaaaaa aatcatctat
     3241 atggtcgtta gctctagtga attaactgaa gctaagagga tcgtccacga gattgacgaa
     3301 aaagctttcc tttcaatcat caatgtgcat gaagtggaag ggcaaggctt tacttatttg
     3361 aagccacaga caagattact gaaaaaacta taagaaaaaa gacaaagata ggctatgagg
     3421 aaagattcca catccagctg acctttgtct tttttagtac tatactattt ttaactaatt
     3481 gttatgttca cttacccggt tacttttctt ttgtgtctgc aaaaggatct ttcggttaac
     3541 ttcatcagat aatttcaaaa aagcatccct atctccatct tctagtgctt gatcgatttg
     3601 agcataaagc atttttagtt ttgcctcttc ttctttttct tgaaaatatc gttcaattaa
     3661 ttcagtatct tcttcactga ctgaagaatc ccaagaagaa aatggattgt cttctaagat
     3721 agatagatat tctcttgtct gccaagcatt ttcgaatata cattccacat ataaaggctt
     3781 tttccaattc aaacggattt catgaaaaat ctggtctgtg tccgtaaatt cttttccctg
     3841 caaaaacaat ttcatgggtt ccccttcaac agatagatca ctgatctgca gtcctcgatc
     3901 cgcaaaattc gccccttcaa caaagtggac attgcttaaa atcgcttcat gattggcaag
     3961 ataattcaag atccaaaaaa cttctcttcg gctaaaagat acatgattga ccatccacgt
     4021 taaaaaattc ttcttttccc gtacatcaat tagcattttt ctcacctctc tgttaggtca
     4081 tctaataatg acagtgcctc catatctcct ggttcaagtg ttaaataata agtcaataat
     4141 tcttgcgctc ttttcagctg cccttcttct cttaagaaaa aggcatactc tttcaagaaa
     4201 tctggttcat gtgatagttc atgataagcc tgttcatagt gtacagctgc aacggtaaag
     4261 tcttctaatt cattataagc atgggctagg ttccattctg cataaggatt agcagtttct
     4321 tccatctgat tgatcacttg tatcacatct tcatatcttt catcatccaa gtaaagatta
     4381 cttaaagtaa gtaatgtttc atcttgtttg tctcctactt ctaaagcttt cagtaaaagt
     4441 tcttctgctc gctgtctatc atgcagacgg aaagcatttt ccgaggccag gtgataaagt
     4501 tcgacttggt aaggattttc tttgattcct tcttctaaca ctgtttgtgc ttcttcaatc
     4561 atttcttctt cttgaagagc ttgtcctaga tatagatata acgattgata atgcggatta
     4621 acttctcgaa gttcttccag atagttgatt gcctgctgat tttctttcaa ttgaagattc
     4681 gtaaaagcta gctggaacag tcgatcatct gtcctttctt ctttcagtgc ttcttctaaa
     4741 ggtacgatag ctgcttcgaa ttctccttgc atacttaagg attgtcctaa acgttcctgc
     4801 atcgagattc ctgaaatttc atttatcccg cttgccaaca atgtttcata aaccactgct
     4861 gcctctttga aacgatcaac tgaaaaatac aattcagcta gcgcaaactg gatcaatggt
     4921 tcatcaggaa gcaacccagc tgcttctttc aattttgctt cactcacttc agggattccg
     4981 ataacttggt acaaatcagc tatcgccaat aagctttgcg gatagtaatc actttgctta
     5041 tccacttttt ccaggtattc aaaagctaag tcgatctcat tatcttcgat tgcaatttct
     5101 gctaacggga tgttcagtcc gtcattttct gggtattggt tgatcagttg ttcaaaaatc
     5161 tgttttgctt cgatcaaaaa gccaatagcc agcaattctt cgcctagttc agctaatata
     5221 tctgctgggt cttcttttaa tgctttttct aaaaagattt gtgcttgtga caagtcttca
     5281 tctgataaag cttgcaacat tttttcgcta tttgtattca tttcaattcc ctcttatctc
     5341 actgacgatt tgttgaaata ccgtcattgt tctcaataat tctttttcgt ttggatgttg
     5401 gtatccgtct attataccta ttttttctaa aacaagtatt tttttgcttt tttctgccaa
     5461 aaaaagaact ttttccaaat actccatctt tgaaatctgt tccagccagt ggattggata
     5521 gccaagcaag tccagccaca caaaaaagtt ttttagttgg aaatcaaaag gatatgcttg
     5581 gagattccac acgaaatgaa acatcattcc taaaaatttt ttcatactag cagaaagtag
     5641 atggccattt tcggtcagat aaaaagcttg ttcaaacagt ttaccataat ttgacaggtt
     5701 ttctgcctct aattgataaa aatagatcat ttcttcgatc atcccggcaa acggccgagt
     5761 cgttaattgc tttttatttg gatagttttt atacagattt tgcaaaaaac gatgatcaca
     5821 aatcattccg caacagataa aagtcaaaaa gtcaatctgc tttccgtcca cctgccgctc
     5881 gctgattgtt tgatcataaa cgatgcgttc tggcagattg atggtttgca gcactacacg
     5941 atttggtagc tgcaaaatcg tccgctgcgg tgttaatgct cggcttagag aacggatcga
     6001 tacagggatc atccatagtg atgaatcaag ggttgtatgt ttttgaaaaa aacctgccag
     6061 atccatgatc ccttcatttc caaatccgat gataaacatg gattgatttt cgggatagcg
     6121 tttgacgaaa tcaagcaggt tctttaattc tgttaaatga ttgcattgag tgttggcaca
     6181 aatataccag tcattgtttg cttgatttgc aaataacggg ttgatcttat ctgcaaacaa
     6241 atcataatag cgctgattcg ttaaaaataa gattggctga tcaatggaaa gttctcttag
     6301 ctgactgact agcgtttctc cgtaaaacac tttcgtcaac gcatcttcta cttgataatc
     6361 aagttccata cgcatccctc ctcttcttat ttttatttta ccattagtaa gcaaattcta
     6421 gaaaagataa gagcaaagct cacctcataa cttggaaata aataaaaatt aaataaaata
     6481 catgtgtttt ctatgtatga catacggatt cggaatctct gtctcttatt tattcctttt
     6541 ctgctaggaa ataaaaagaa aagcacttgt agccctagag ccacaagcgc tttttcaact
     6601 gtcttattta acagcgtctt ttaatgcttt acctggtttg aatgcaggta ctttgctagc
     6661 tggaatttcg atttcttcac cagtttgtgg gttacgacct ttgcgagctg cgcgttcgcg
     6721 aacttcaaag ttaccaaaac cgattaattg aactttttcg ccgttagcta aagcatcttg
     6781 aatagttgaa aatacagcat ctactgctgc agttgcatct tttttagtta aatcagtagc
     6841 tgaagcaact ttttcgatta attctgcttt gtttgccatg gatagattca cctcctccac
     6901 aagagatatg atagaaatat catttagtta aaactttctg agtggtccag aagtttaaaa
     6961 agaaatattg attaacttca atattctgtt atcacgatat catatgaacc gcttaatagc
     7021 aaggttttta ccaattttca catgcttttt ttagtcatag aaacgtaaca aacgaaactg
     7081 tctttttctt ttgaaaaaaa aacgtcgata tggtaaaatg cctattttct cctacgaggg
     7141 ataattcgga tggacgtccc ttcaaaagta aatgcttttc tgatttgatt ttctaagaaa
     7201 cgttcataag aaaaatgcat caattcctct tcattgacaa aaacgacaaa tgtaggtggt
     7261 ttgacagcga cttgtgtagc ataaaaaatc ttcaggcgtt ttcctttatc tgttggtgtt
     7321 ggattgattg ctacagcatc cataatgata tcattcaaca cagccgaaga gatacgaaga
     7381 ttctggttca tacttacttg ttcgatcatt tcaggcagac gttccaaccg ttgtttagtc
     7441 actgcagaaa caaaaacgat tggagcgtaa tcaagatacc ggaattcatc acgaatctct
     7501 tgttcgaaat cacgcattgt attggtttct ttctttacaa gatcccattt attcacgacg
     7561 ataataattc cacgaccagc ttcatgggcg taacccgcta ctcgcttgtc ttgttcccga
     7621 atcccttctt cagcattcag taccatcaaa acgatatcag aacggtcaat ggcacgcatt
     7681 gcacgcatca cgctgtattt ttcagtattt tcgtatactt ttccgcgttt acgcatacca
     7741 gctgtatcta tcattaagaa tttctgacca ctttcagaaa cgaagtgggt gtcgattgcg
     7801 tctcgggtcg ttccttcaat atccgaaaca attacacgtt cttctcctaa aatcgcattg
     7861 atcaaagagg atttaccaac atttgggcga ccaatcaaac taaacttaat cgtgtcgtcg
     7921 tcctcttctt cagtctcttc agagaaatac ttcactgctt catctaaaat atcaccaatc
     7981 cctagtccat ggcttcctga aacaggataa ggatcgccta gccctaagga ataaaattca
     8041 tagatatcgt ttctcatttc tggattatct actttattga ctgctaaaat cactggttta
     8101 ttacttctgt ataaaatttt cgcaaccatc tcatctgcgt ctgtcacgcc ttcgcgtccg
     8161 ctgactacac aaatgattac atctgcttct tcaatcgcga tttctgcttg atgcttgatc
     8221 tgatccataa aaggttcatc gccaagatca attccgcctg tgtcgatcac gctgaattct
     8281 cgacctaacc attcaccttt tgcataaata cgatcacgag ttactccagg agtatcttcg
     8341 acaatcgaga tacgctcacc agcgatgcgg ttaaaaatcg tagacttacc gacgttcggg
     8401 cggccaacga ttgcaattgt tggatttgcc atattatttc ctcctcacaa gtttccaatc
     8461 tcacttagtt taacgaatgc attgccaact ttcaagcata gattgtcaat taaacgaagt
     8521 atttttattc cttattgaaa tactaatcgc ttttcaaaag aaaaactgag acaaaagcag
     8581 ctgctttcat ctcagtcaca gatacaaaat caattatttt tcagaatcat cggttgcttt
     8641 ttcttctact tcttctttca acgcatcacc taaaatgtct cccatagtaa atccagtatt
     8701 ttcttctggt aattcataag actcaacatt ttttggttct tcttctgaag ctggtttttc
     8761 ttctaaagct ttgatactca atgcaatacg atgttcttct ggatggatct ctaatacttt
     8821 cactttcact tcgtcacctt catgaagcac ttcatgtggt gtcgcaatat gtttatgtga
     8881 aatttgagag atatggacta gcccttctac tccagggaac acttcaacaa aggcaccgaa
     8941 gcttgtcaaa cgtttaactg ttccgtctaa tactgtgcca actggagctt ttgtatcgat
     9001 ttctgaccaa ggtccaggct gtgtgtcttt gattgacaag gaaacacgtt cttgttcagg
     9061 attgatagac aacactttga catttacatc atcattgaca gccaatacat cacttggttt
     9121 tgctacatga ctatgtgaaa tctctgagac atggactaga ccgtcaattc cgcctaggtc
     9181 gacaaatgcg ccaaaatcag taagacgtgc aacacgtcca ctgagaacat ctccctcatg
     9241 gatagttgaa agaatttctt ttttcttttc ttctctttcg ttttccacta ccgctttatg
     9301 cgataagatc aaacgatttt ctgatggttc gatttcgatg attttaaagc tcaatgtttt
     9361 gcctttatag tctgaaaagt ctgcaacaaa atgatcttcc accattgatg cagggacaaa
     9421 accacgtaca ccgacgtcaa cgactaaacc gcctttgaca acatttgtaa caggtgcttc
     9481 gatgaccttg ccctcttgga aatctctttc gatatcttcc caaacttttt tagcatctaa
     9541 acggcgtttt gaaagtaggt agctgccgtt ttctttatct ttgccgatcg tactgataac
     9601 gactaaatct aatacatcac cgacttttac tacttcgctg atatcttcta ccggcaaagt
     9661 tgataattct tttgccggaa cgactccttc aactccagcc ccttcgattc cgacaatcac
     9721 ttgtttgtct tcaatgacta aaacttcgcc cttaacaaca tcgccaactt ttacttcttg
     9781 tacgcttttc attgcgtctt ccattgactg gttgttctct tgattttgat caaattctgt
     9841 cataacactt gtcctcctaa ccaacattct gccgtaagta cggtactgtt tttagtttaa
     9901 ttgattattc aaaaaaaata aagcgatttc gttcgttttc cttgaaaaaa caattgtttt
     9961 tcattttttt tcttaattta aaaaagaaga taaccttttt tcaaaacaac agcttttatt
    10021 gctgcaacga cttctggaat cgatttgcca gtcgtatcga tacgaacagc atcctctgct
    10081 tgcttcagcg gagagacgtc ccttgttgaa tcataatggt ctctttcctg tatctctttc
    10141 gtcagtgttt caagatcagt cggaattcct ttttcttggt tctctttgaa acgtctttct
    10201 gcacgctcac ttacgcttgc tactaaaaag attttcactt ctgcattcgg taatacagct
    10261 gtcccgatgt ctcttccgtc catgacaacg ccaccgcgtt cccctatttt tcgttggaca
    10321 gcaactaatt cttctctgac ttttccatgt gcagagactt cagaaacagc ttttgtgaca
    10381 tctggttgac ggatttctaa agtcacatct tttgatgcta tgaatacatg ctgtccatct
    10441 tcttgttgcg aaaaagagat aggatacttt ttgatcagcg aaactagccc ttcttcatct
    10501 gaaaaagata cattgttcgt aatagctaaa taagttactg cgcgatacat ggcaccggta
    10561 tctgtataaa tataattcaa ttcttttgct aatatcttgg caacggtact tttcccagat
    10621 gaagcaggcc cgtcgattgc aatactgatt tgtgtcatga tataactcct ttttctcaga
    10681 aaaaaagccg caaagcgcgg ctattattat ttaattctta attcttgacc agggtaaagc
    10741 ataaagttgt ttggatctaa gccattcaat tcaaacaatt gatcagtggt gatccctgca
    10801 cgctcagcca cttgttttgg accttcgcct gcttgaacag tcgttgttga accggctgca
    10861 gctgatgaag aaggtgttgt ttctgctgca ttgctgcttg aatcccctgc ttgataagtt
    10921 gcctcacttg aggaagtcgg tgttgtctct gctgcaatac tgctttctga cgttacacct
    10981 gtatcttgag acacagtaga ttcttccgcg attgtactag attctactgt tgaagtctca
    11041 gttgactcct cttttgttgt acttgtagaa ctttctacta ctgatgtcga agtttcagaa
    11101 gaagttgctg ctgttgcatt gttgcttgat ccatttctga tccagaaata agcacctgct
    11161 ggaatagcga tacataagac cagtaaaatc aaaaatagcg aaagaaataa tgtatttcct
    11221 ttcttttgtt gtctctgaga acttcttgat gaaacttcat catctgtatc gtagattggt
    11281 tgttcccatg gttcttgtgt ttcgttattt tctgtagatt gatgtctatc ttttctgctc
    11341 actgttttac ctcctaaact ctatcgtgtg tattatacca taggaaaaag tgtcttgtcc
    11401 taaaaaatca atgttccatg ttttttttaa ataaatttag taaaatcgtt tcccaatgaa
    11461 gttgttcagt cttagaaaaa agcttcctct ttggcagaag ttctgctttt gctccatcaa
    11521 tatcacaaca attctgcggc ttttcggaga gtttttcgcc aaaataagct aagatgaatt
    11581 ttctgcggca gttttcttca ttaatatagc gaagcatctg ctgcaggcgg aattcttttt
    11641 ctttttcctg acgtttgagc cgttccaccc aattttctgg tttttcagac tgctggatca
    11701 gctccatcca ttttttttgc agctcatcaa agggagcttg ctttgtggca tattccaaat
    11761 aaagctcaaa actttgtcgc tgttctctag aaagttgatt aaagaaataa tggatacgtt
    11821 catccccact ttgataaaaa agaattgctg cactttcttc ctgatctctg cctgctcgac
    11881 cgatttcttg tacatagttt tctagactgt ctggcaaatc ataatggacc acataacgga
    11941 tatcagattt atcgatcccc ataccaaatg cattcgtagc aatgagaaac tgcagctgat
    12001 tttttacaaa ctgctgttga agttgtcttc tttgagctgc atcaagacca ccgtgatagt
    12061 atccaactgt aaaacgttcc ctgaataaat gatacaatcg ttcgacttct ttttttgttg
    12121 cacaataaat gattgccgct ccttgtgctc tttccatgaa gtgttcaagt tcttgttctt
    12181 tttgctgcgt tttccgaaca aaaagcgaga tattttgccg attgactgat tgtctgatct
    12241 cttttggtgg tcgatcaaat agtacatgac gtatatctct tgctactaaa tctgttgctg
    12301 tcgccgttag tgccagtgtg accgggtttc ctaaaagctt tcggattttt cctaactgtt
    12361 gatattctgg acggaaatca actccccatt gagacacgca atgagcctca tctactacat
    12421 ataacgcaat attttgtttt ttcagatgct ctaaaagaga aggattagtc aacatttcgg
    12481 gactcaaaaa caaaaattta tattgcgata gatgacggag gacgtatctt ctttcagatt
    12541 gcgaaagtaa actattgaaa gcagctactc gtttctcccc tcgtttttgt aattgcgtca
    12601 cttgatcttc cattagagaa agcagcggtg acacgatgat gaccagacct tccattaaat
    12661 accccgtcaa ttgatagcaa aggctttttc ccgttcctgt aggcaaaata gcgagagtat
    12721 cttctccatc aagcaacgcc tgaataattt cttcttggcc gggacgaaat gaatcaaatc
    12781 caaaatattt ttttaattca tttattaaca ttctgtatcg cttcctttct caatagatag
    12841 atttgataca aacgaaattc tccataatct aattgtccat ctacattcca ttcgctaaag
    12901 cgccactctc gcacggaatc cttatttggt tccaaacgtt cgattgtttc tgcagagaga
    12961 atccactcgt atggaaaatc atcaaaaaac aaggcccact caataagatg atctgttact
    13021 gttcctcttt ttaaatgacg gattgccatg atctggtcaa ttgtctgacc attcataaac
    13081 atctgcttgg tgatcatcat gctttgattg aaattttgct gtaataatga atcgatcaaa
    13141 gcgtgccaaa gcgaatctgg ccgtttttcg atttgtgcta acaaaaggtg gatacatctg
    13201 ctttgttgca gataaacctc tgactcatca ttgttttttg ccagctgata aggaagcagc
    13261 cctgttcgct catttccaga taattggtta gctaaaaaat ctgctgcttc ttctggtatc
    13321 tccgaaaaca gctgcaccaa actctcatac gcagtatcaa tcaaaatttc acgtggcaat
    13381 tggctttcac taagccattt ttttagttgg aaagtataaa aaggacttgt ctctgctgga
    13441 agatagtctt gaatacccgt tgccagatta gaaattactt gaacagcaaa tttaatgagc
    13501 cgccaactag tttcccctgt tctgccatag cggtcaaaac gcaaaccagt atgttctatt
    13561 tgatgttcgg acaacatatc agcccctgca ggcgttagct ttgcttcatt ttcatttatt
    13621 tcgatccaac cttgctgcgc aattttttgc aaaatttgat aaaacttatc ctgcttcaat
    13681 tctggaaaac tgttatggat aaacaaaagt tcgtgggtaa atccataaat aagtactgaa
    13741 gtcgttcgtt ttcccacaag gagttggtaa agagaactaa tccgcagctt gtgtcctgat
    13801 gaaaatagcg ataaaataaa aacaatcata ttattctcct taagttagga tggttagaaa
    13861 tgtcactact atgtaaactt gtacctgaac gctgtattgc atgcggtctt tgtcaaatcg
    13921 aagcgccaga tattttcgat tatgatgaag aaggaatcgt tttatttgcc caagaaccag
    13981 aggctcatca acagttcgtg atgccggaag aagaaacata tgtaaaaaaa gcgtatcaaa
    14041 aatgtccagt tcgagcgatt ttattagaaa ataaaatata aatataaacg aaactttaaa
    14101 aacgtacact aagttctcta aacttgaaaa taagccggga tctgtttctc ttgttccgtg
    14161 aaatcccgac ttattttgaa agattcattt gctatctata gttaattgac tagttttttc
    14221 acggacccta taggacccat ttatattatt tcaacttttt cagccagttt tccaatgtat
    14281 tgacgatcac agtttgctgg tctgcgttag aaatttctgc ctctccatct cctttttgct
    14341 caccatagct gccaaaacct ccatgatttc cgccattgat tgatacatac tctgttcttt
    14401 ccggtaaata ctgcttagct tcttgataag cttcctgatt caatacttta tcttttgtcg
    14461 ccgtgatcga taaaacagga acagctgatt ctgtcaatgt tccttttttg tcgggataac
    14521 ttgccatgaa gaatacccct tttaaatgtt tgtctgaatg attcttattg acataacgac
    14581 ttgccattac gccacctaat gaatggccac cgatcacata atcggaattt ggaaaatctt
    14641 ctttgacctt atccgctttg tttccgccca tcactgctag atcaagtggc attttgacaa
    14701 tagcgactga aaagccgttt tttgctaaat cttgcgccca aatactataa ctttctggct
    14761 ctacgagagc accaggataa aaaataagta atggagactt tccatgattc tttttctctc
    14821 cttcaaaaaa taagtaatct tctttgtttt ttccttcttt taccaacact tgcgcttctg
    14881 acgatggttc gtacgtattt tgctgtatat agtaataacc ggctccaccg agtaatccta
    14941 aaatgatgac taagccaatt agtattcgtc taaatatttt cactttctcc caccttttct
    15001 atctgagtga cccctttttc ttaatcaagt atgattatac cttaaaaaaa taagagagaa
    15061 atgcaaactt tccagcatca aataaaaaaa gacggaatat ccatttgaat atccgccctt
    15121 tttgtatgtt tatttttatt tagcacataa acgatacaca gcttctgcat aaatttccat
    15181 tgctttatac atactactta attcccatcg ttcattcgcc tggtgcataa agtcaggagt
    15241 atcagggaac atcgcaccga atgccacaca ctggttcatc gtacgagcaa aagtagcgcc
    15301 gcctgataca aacggttcag tcatgtctcc agtaatatcg cgatatgtcc ccaatagatt
    15361 tttcactaac tcactgtcta gtggcacgta taatgaatca aggaagtcaa attcttcata
    15421 agtcaaccca tatttttctg ctgttttagt taatttagct actaaatcat ctttcttgaa
    15481 tgtaacaggg attctcatat ctacaccgat ttttgtttgt tctggtgtga tttccaaact
    15541 tgcaaagttc atcgttagct cgccggattg ttcatcttct gttttgccca cgacagcttc
    15601 accagttgca ttttcttgaa ctaattttcc taagaaattg attgggccaa aatcaaatac
    15661 ttctgaaaga gcaattgcca gacgagaaat agcattcact ccttgtgctg catctttcgc
    15721 gtggatactt ttaccaagaa cgacgatgcc ttcaccttgt tcttcgaaat cgaattcgtg
    15781 ttttttcaat gcttctttta cttcagaaag cttttctcca gagtaaggag ctgcatcagg
    15841 aacaacattc aacgctcctc cagcttttac agaaaactcg ttcgttcctg gtcctgtcaa
    15901 gtaagcttgc aataagcctt tttctgcata aattaaagga aattcagcat ctggcgcaaa
    15961 tccttgcgta attccttctt ctttttcgtt atatttttct aaacaacgcc ataaattttc
    16021 ttcatctgta ccaaagataa aacgaatacg tgtcttgaat tccacgccag catcaatcaa
    16081 agctttgacg gcatacattg ccgcaataga aggacctttg tcatcttggg atccgcgacc
    16141 aactaaccag ccgtctttta tagttggatc gaatggtttt gtttcccaat tattttcgtc
    16201 ccctgctgga acaacgtcca tatgacacaa gataccgaat aattcatctc cagagccaat
    16261 ctctgaataa ccgtaatagc cttctggatc tttaaatgtt ctaaatccta gcttttcact
    16321 gatttccaat actttatcca aagcttcaat cactttttta ccgaagggat gtccttgtcc
    16381 tgaatctgct tcatctagta cggaaggaat tcggatcaat tcgctcaatg attctaatgc
    16441 ttgttcatga tgttgttctg tcacaaattt tttcatgata ttatcctcct aatatgttaa
    16501 aaagggtcat gtcaaattcc tccgacacaa cctcttcttc tcaaataatg acgttttaag
    16561 cctgctccat cagcaagcag aatgttctta acaaatgatg aaagaaaaaa cactcactga
    16621 atggagctat tgacttgtcc acgtgcttat cgatagccgt ttatcggttt cttcattctt
    16681 atgatagacc ttgtacctgt atttgtctag gtttagaaca ttgctgctac acctaggatc
    16741 acacagatgg ctacaaataa tatcgcaatt aatttaccgg taaatttcca ccacactgaa
    16801 acatcgaaac gagcaattgc tacagcaccc attacgatag cagatgttgg tgtaattaag
    16861 tttactaaac cagatgcaga ttgataagca gtgattacta aatccttacc gacgccggca
    16921 aattcaccca taggtcccat gattcccatt gttgcagcag ctaagccgga agttgaagga
    16981 atcaagaatg acatagggat atagaaaata aatgtaataa tgatgaatac acttgaagat
    17041 aatgaactta atccttgttc gccccaatgt aagatggtgt ctgtgattaa tccattgttc
    17101 ataaccactt ggataccacg agcaacagct actacgattg ctacaccgat caaatcagaa
    17161 gcaccagcta aaaattcaga gacaaattca gactctttca tgccataagt gattgctact
    17221 acgatggaca ttactaagaa taacatagta atttctggga agtaccagtc acctaatgga
    17281 agcatgcttt tacctagaac cgtacctagt accggaaggt ttgttaacca gtttgtcaat
    17341 gattcaaaaa tcgtgaaatt cgcattcaag gttgtccaag gaatcaaact gatgatcatg
    17401 ataccaaaag taataaagaa caatacatta acatgttttt gttttttcgt gattacttct
    17461 tgacgaccga ttgcttcaat atcgaaatgc ttcatatctt ctttacgttg gttgtatact
    17521 aatgattttg ttggatcttt ttcgatcttt gatgcataac gatacacata tacaatagaa
    17581 atagctaaca aaataacgaa catcaagaat cgaagaccaa taccttcacc catcgaaatc
    17641 ccaacagctt gtgaagctac gcctgttgca aaaggattta ccgttgaagc tagacagcct
    17701 acttgagacc ctactaatac gattgctaca gctactactg tatcaaagcc tacagccatc
    17761 atgactggga tcagtaaagc atagaatgga atcgtttctt ctgccattcc gtaagtagag
    17821 ccgcctaatg caaataacag catcaatact ggaataagca ttttttcttt tcctttgaat
    17881 ttcttcacaa ctgaagcaat cccggcatct agtgcccctg ttttgttgat aatacctaaa
    17941 aatccaccaa caaccaaaat gaagaaagag attgggattg cagcaggtgt ttctttagtt
    18001 agctcccccg ctggtacacc tagcatccca ttgattggcg ccatgaaaat atcccacagc
    18061 ccttgaggat tttgttgtac tcgttcatat gatccagcga cgatgttgcc tgcatcgtct
    18121 acactatagt gcccagctgg tataaaccaa gtaagtacag cgattactgc aataataata
    18181 aataagaccg tatacgctga aggcatttga aattttttct tttttgtttg ttccatcttc
    18241 ttcttccttt cttttccgtt agagacaaca attttggctc taatgttatt ttatcaaaaa
    18301 gagtatcaga acaaaaataa taacacgcat tttgtaaaac ttttgttcaa aaagttcgtt
    18361 tttccctatt ccctcctctt ttttcagttt tttaaagctt tcttcttttt cattgtagcg
    18421 tttccttttt tttataaaca aacattttcg tcttttaaaa ggagaagaat ttcacatttg
    18481 caaagaattg attgattttt atgcataaat caaaaaaata ctcaaaacaa acaaataaaa
    18541 cgtttacatt attgcatgtc ttgtattttt ttccaaaaaa aaagaccaag aaatttcttg
    18601 atctttttga tatttatttg actgctgtat gacgttgttc gaactgatgt tgttttcgac
    18661 ttaaccatgg aagaagttgc gcatgcagca ctaaaaagac agcactcacg atgaatcctt
    18721 tgatcaagtt gaaaggaacg atcccaatca gtacatagtt tgctaaagac atgcccagca
    18781 tctgtgttgc gttcagccct aaaaacatta agtaaagagg cgtaataacg aaataatttg
    18841 cgatactcat aaaaatagtt agagcgatgg ttcctgtaat gacacctaaa aatttgtttc
    18901 ttcctttatg ctgtttcttt acactaaaga aataataaat cgggaatgta aagatcgttg
    18961 ttgataagaa actagcagtg tctcctacca aattatccgg agaaaaacct gtagtcagca
    19021 gatgtaatac tgaacgcaag aacgctgttg caactccggc cgctggaccg aataagaaca
    19081 tgctgatcaa taccgagata tcactaaaat cgatcttcaa aaaactaaaa gccggcatga
    19141 tcggaaaagc aaaatactgt aaaactaatg cgatcgcagc aaacatcgct actgcaacca
    19201 ttttctgtac acgggtgttc ttcatttgaa atcctcctgt taggactcct cttcagcgaa
    19261 ctttttgacg tttctctcca aatgaaaaaa ctctattttc cagggaaaat agagttagag
    19321 ttatttgatc aagcgtcgtc aaataagctc tatcttcttt atccagacta tactgtcggt
    19381 atcggaattt caccgattca gctatttttt tgcaaaatag gtcgtggact ataaccaccg
    19441 gtcgggaatt tcaccctgcc cctgaagacg aaccttatat aatttttgtt tacatattca
    19501 ttatacatat ctattcaaaa aacgcaacat ctaacaatta taaaaagtaa gtgttcattt
    19561 tgagtggttc agcaaactgc tgacttcttt tttgttcagt tcacggtaat ccccaggacg
    19621 taggccttgt aaagtcagat ctccgtattt ttcacgtttt agtttttgca caggaagacc
    19681 aactgcttga agcatatttt tcacttgatg attacgtcct tcgtggatcg tcaattccac
    19741 gatactgtgg tttgtcttag gatccactga taaaatttgg aatttagctg gagatgtttt
    19801 tcgtccttcg atctttattc cttttgtcaa aggtgcaagc atcgattttg tagcgatccc
    19861 ttttaccttt gcaacgtaaa ctttatccac ttcatgtttt ggatgtgtca ggcgctgagc
    19921 aaaatctcca tcatttgtca gtagcaagat ccccgaagta tcgtaatcca gtcgacctac
    19981 aggatagata cgttctttta ccatcggcaa aagatccgta acaacttttc ttcctttgtc
    20041 atctgcaaca gcagaaataa ctcctttagg tttgtacaat aaatagtaac cgtattcttc
    20101 ttgataaatt gggacttcat ccacttccac agtgtcatgt ttcccaactt ttatccctaa
    20161 ttcccttaca acttgtccgt ttacttttac acgtcctgca aggatgaatt cttctgcttt
    20221 tcgacgagaa gtgatgccag cgtgggcaat tactttctgc aatctctcca ttacaattat
    20281 tctcctttat tcattccatc aaagaataga tctggtgaca cgtcttcttc aagtgaaatt
    20341 tccatttctt gtgtatcagg cagttcttct aatgatttca aaccaaaata atctaagaag
    20401 tagccagttg ttccatataa tatcggccga cctggtcctt ctactctacc tttttcttca
    20461 atcagtttat gggcagctag acgctgtacc gctcctgaaa cctgaactcc tcgaatctgc
    20521 tcgatttcag ctcttgtgat tggctgttta taagcgatga ttgctaaggt ttcgacggcg
    20581 gcttgagata atgtgtttga tgtcccttgc gcatatcgct taagtaaggg agccagctct
    20641 tttttagtcg ttaatacata atggctttcc gtttcaaaaa tatgcaaagc tgagtgcgag
    20701 ttttgagtgt aagattcttt taattctttc actaaacttg tgacttctgc ttcgctgaga
    20761 tttaacagtc cgcttaattc ttcattactg atcccttcct cgccaacaat aaaaagaagg
    20821 gcttcaagtt cacttaattt cgtcatgttc ggtatctcct actgctggat aaagcataat
    20881 tgattcatag ttttcttttt gttgcgcaat gatctgccct tttttcatca attctagcaa
    20941 agccataaaa gtagtgacaa cttcactttt agaaggtgac tcaaaaaaag aatcgaaagt
    21001 acagccatgc gcatttcttt ttatacgctc ttcgataaat accattcgtt cttcgatcgt
    21061 tgtgtcatcg ggcgtgatcg ttttttccac tttttgagaa aatttcttac gttctaagat
    21121 tttctgcatg gcacggatca aatcgatttt tttgacttgt cctcgcttca acggcttttc
    21181 agcatccgct aacccatcga tgttcatagg ttcttttgtg taataaagac ttcgttcctc
    21241 tgctttttct gtcagtcttt cagctgcata tttatattta cggtattcta aaagctgagt
    21301 gaccaattct tctcgaggat cttcttccca taattcttca tcgtctgtgc cgaattcttg
    21361 gacaggaagc aatgtcttgc ttttgattgc catcaatgta gcagccatca ctaagtattc
    21421 tcctgcaaca gctagctcga gtgttttcat tgtatggata taattcatat attgctccgt
    21481 gatcgtagcg atcggtatat catatatatc gatttccatt tgctgaatca gatgaagcag
    21541 cagatcgaga ggaccctcga atacatctaa tttaatattg atctccgtca actcattcac
    21601 tctttcttct ttttctttgc tagccatttt ccaatgtaag gctctgtgat ttcagttgca
    21661 cggatggaga catccgggta cttttcctgt aattcatcta acatttgaaa gcccatattc
    21721 tcaccgcgat aagacggatt caaagaaatg tcatggatga tcagctgatc gttcgttgcc
    21781 cagtaaatac caataatccc ttgaatgttg gttgaacctt ctggcgtaaa gaaaaaaagt
    21841 tcataatcgt cttcagattc gtacgtatca atctccttca acaaagaagt tttacctgtc
    21901 agtttcttat ggaaactcaa tagtcccata gcaattttac ggttttcttt ccggtaatgt
    21961 gttaacatct tctcaccttc ctatgactaa gcccttggaa aatgctgctt ataaacatcc
    22021 gtcattcttt tcttcgtgat atgtgtataa atctgagtcg tagagatatc cgcgtgacct
    22081 aacaattcct gtactgtccg caaatcagct ccattttcta gcagatgtgt ggcaaagcta
    22141 tgccgcagcg tatgtggtgt tacttctttg taaatcccag cctctcgtac taactgcttc
    22201 agatttttcc agattccttg ccgagaaaga cctttcccat gattattgac aaatacatgc
    22261 atttcagatg cgtcctttac aagtaatgga cgtgcttcgt ctaagtagcg ttccagccat
    22321 tggatcgcat aatcacctaa aggaatgatc cgctctttat ctccttttcc aactgtctgt
    22381 accagtccaa gagaaagatg aagatctccc aatttcaatc caatcaattc actgacgcgc
    22441 atcccagtag catacatgac ttccaagatt gcacgatctc ttatccctaa cgtttttgtc
    22501 gtatcaggtg cttcaatcag cttttctact tctgtcagag tcaatgtact tggtaatttc
    22561 tgtgctttct tcggcgtatc gatatgttgc atcggatcat gatcagtgat acgctcttgc
    22621 cgtaaaaact gatggaatct tctgagactt gagatcatcc gagtaacagt tgctgtcgct
    22681 tttttttctt catgcaactt ctccagaaaa cggatgacta aaaagcgatc gacatcctgc
    22741 catgaagcaa ttttctgctc ctgtagaaaa tgcaagtact gttccaaatc acgttcatag
    22801 ctttgtcgtg tattggcgga caatccccgt tcaatattca agtagtggag ataatcgttg
    22861 atttgctctt tcataataat ccccctcatg cctctctatc atatcagata ccgcttcatt
    22921 ctgatagtct ttttccataa tctgagcaaa gaaaaagccg tctagacact cagacggact
    22981 ggtttctttt
//
LOCUS       DACEEZ010000041        22342 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20313_2017
            SAMN12639966-rid9879223.denovo.041, whole genome shotgun sequence.
ACCESSION   DACEEZ010000041 DACEEZ010000000
VERSION     DACEEZ010000041.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639966
            Sequence Read Archive: SRR10023158
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 22342)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22342)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741683.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741683.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:44:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,016
            CDSs (total)                      :: 2,962
            Genes (coding)                    :: 2,889
            CDSs (with protein)               :: 2,889
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 36 of 73
            Pseudo Genes (incomplete)         :: 29 of 73
            Pseudo Genes (internal stop)      :: 18 of 73
            Pseudo Genes (multiple problems)  :: 8 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22342
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639966-rid9879223.denovo.041"
                     /isolate="E20313_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(218..1015)
                     /locus_tag="IWO89_001595"
     CDS             complement(218..1015)
                     /locus_tag="IWO89_001595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TPM domain-containing protein"
                     /protein_id="HAQ0456343.1"
                     /translation="MMKKFFLFFLLLVSVGWPVATHADTATVNDEAGLFTTDQIQSLE
                     QQAEKLNEKIKGRVLIVTTTSNSEEPRDFADNYLRDAVGNDQNGSVLLLDMGQREIYI
                     STSGNMIDYLTDSRIDSILDDVYDQMTNAAYYNAAKAYLSKSATYVEDGVPGGHYRVD
                     EETGKITRYKVLTTVEIIIAVVLALILSLVFYFVTVSRYQLKSGIYKYPFREKSSIKL
                     TEKTDRLTNSFVTTRRIPKSPPPGSGGGGSTTHSSGGGTFGGGGRSF"
     gene            complement(1012..2196)
                     /locus_tag="IWO89_001596"
     CDS             complement(1012..2196)
                     /locus_tag="IWO89_001596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TFIIB-type zinc ribbon-containing protein"
                     /protein_id="HAQ0456344.1"
                     /translation="MDVLTHKCPNCGGPLTFDPKDQKFHCEYCLNIYTEEEVSQYEQK
                     QKEARMAGEESKEEPSPTEDFTFTAQEQLADMNEAERKAVDEAGGFSDTADESADNTA
                     AIDLFLCPNCGAEIVTDATTAATYCYYCHNPVVLSGRLSGEFLPNKVLPFAVEKEEAI
                     NRFLAWTKKKWFVPKAFFNKDQIDKLTGVYFPYWATDAEVDGAMQANGTVIRIWRIGD
                     IEYTETKQFAVSRAGKLSFKELVKNALSKNTQQKMVEGVQPFPLDKAIDFKSQYLAGF
                     QAEKRDIEYEAIKSQIQSELKDYSEKLLKDTANGYTTLTGVRTSASITNEVNNYVLLP
                     VWLVTYRSNDSSKKVYYYAMNGQTGKVSGVLPISQKKLGLTALGIFAGLAILFLIGGY
                     LI"
     gene            complement(2221..3468)
                     /locus_tag="IWO89_001597"
     CDS             complement(2221..3468)
                     /locus_tag="IWO89_001597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="virion core protein"
                     /protein_id="HAQ0456345.1"
                     /translation="MGLIKAATSMVGGGLADQWLEVIEPDNMSDTTVMTKGVKVRKDD
                     KRGSNRKGTEDVLTDGTVVHVYPNMMMLLVDGGKIIDYTAEEGYYTIKNDAAPSMFNG
                     TLKEAIAETFDRFKFGGVTPQKQQVFYINLQEIKGIKFGTSAPLNYFDNFYNAELFLR
                     AHGTYSIHVVDPILFYTNAIPKNKTQVEINDINEQYLAEFLTALQSAINQMSADGQRI
                     SYVPSKSLELSKYMDTALDDSWRELRGMEIVSVAVASISYTDDSVKLINMRNEGAMLG
                     DPSVREGYVQGSIARGMEAAGKNEAGAMTGFMGVGMGMNANGSYLSQAAQNNQEQIKQ
                     QAEKQNQQTAQGNADTWTCPVCGTENSGKFCSNCGAAKPVENVQPKLQMRCSECNEIV
                     DLSNGIPKFCPNCGKPFKGIPVN"
     gene            3683..5428
                     /gene="dnaX"
                     /locus_tag="IWO89_001598"
     CDS             3683..5428
                     /gene="dnaX"
                     /locus_tag="IWO89_001598"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HAQ0456346.1"
                     /translation="MAYQALYRVWRSQRFEDIVGQKAVTQTLKNAIVSHKTSHAYLFT
                     GPRGTGKTSAAKIFAKAINCPNSKDGEPCNECEMCRSITAGTQEDVIEIDAASNNGVE
                     EIRFIRDRANYAPTKAAYKVYIIDEVHMLSTGAFNALLKTLEEPKESVIFILATTEPH
                     KIPATIISRTQRFDFKRINTADIVEHLEFILKESTISYEPAALNVIARSAEGGMRDAL
                     SILDQAISFSEGTITLDDAMQVTGSLTDEMMDSYLSACVKKEVPEALETLNQILAAGK
                     ESRRFLEDMLQYCRDLLMYQQAPNLVEEQSSTIRSTFTELAQTVSADQLFSMIKILSD
                     TQNEIRFTNSAAIYLEVATVKLAQLPSASVPSADVAFADDQIVKQLQQEIKELRNEIK
                     ELRSNTGIENTKVQPVQQTNHKKQTSTYRVPTERVYKVLREATRQHLNQVKEVWEDLL
                     MSLSVTQRAMLKASEPVAAGPTGLVIAFDYEIVCQRAANDEELQLSVHNNLSRMIKDY
                     APDPVYITRESWPELRRNYLTQAQEEGTENSFASEDSEGEIELLPQEQEVPEETLVVK
                     AEELFGSEAVTVVDD"
     gene            5448..5762
                     /locus_tag="IWO89_001599"
     CDS             5448..5762
                     /locus_tag="IWO89_001599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbaB/EbfC family nucleoid-associated protein"
                     /protein_id="HAQ0456347.1"
                     /translation="MMRGMGNMQGMMKQVQKMQKEMAEAQELLNKTEFTGKATNELVK
                     AVFTGDRRMKDIQINEEVVDPDDIEMLQDLVVMAVNDAIAQIDVETEKKLGKYAKGLP
                     GF"
     gene            5787..6383
                     /gene="recR"
                     /locus_tag="IWO89_001600"
     CDS             5787..6383
                     /gene="recR"
                     /locus_tag="IWO89_001600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination protein RecR"
                     /protein_id="HAQ0456348.1"
                     /translation="MQYPEPIAKLIDSYMKLPGIGQKTATRLAFYTIDMKDEVVNEFA
                     KSLLSVKRDLHFCSICGNITEEDPCEICRNPSRDRSVILVVEEPKDVMAMEKMREYHG
                     LYHVLHGVLSPMEGTGPEDINISSLLQRLHDADVKEVIIATNATTEGEATAMYLSRLI
                     KPAGIKVTRLAHGLSVGSDIEYADEVTLLKAVEGRREL"
     gene            6447..7091
                     /locus_tag="IWO89_001601"
     CDS             6447..7091
                     /locus_tag="IWO89_001601"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HAQ0456349.1"
                     /translation="MNGLFITIEGPDGAGKTSILNELYPLLKEVAKTEIIQTREPGGI
                     PIAEEIRAVILDPKNDRMDERTEALLYAAARRQHLVEKVLPALAKGKIVLCDRFVDSS
                     LAYQGAGRRIGVTEIARLNEFATEGTTPDFTLYLDVDSDTGLRRIKKNRQNQIDRLDS
                     EGLEFHQRVRHAYLKLAEENPERIHKVDARKSFEEVLQTSYHTIIEQYPQFFEN"
     gene            7106..7435
                     /locus_tag="IWO89_001602"
     CDS             7106..7435
                     /locus_tag="IWO89_001602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016172581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0456350.1"
                     /translation="MKIILAIIQDKDSNRLSNELIDANIRATKLSSTGGFLKAGNSTF
                     IVGIEDDRVEEALEIIKKTCESRKQFVSTPVTLDISMDGGVPYPVEVEVGGATVFVLP
                     VEGFHQF"
     gene            7449..8387
                     /gene="holB"
                     /locus_tag="IWO89_001603"
     CDS             7449..8387
                     /gene="holB"
                     /locus_tag="IWO89_001603"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta'"
                     /protein_id="HAQ0456351.1"
                     /translation="MNQEINLAQMQPIVYQQLQRSFEHGRIAHAYLFEGEKGTGKHEV
                     GIWLAQHLFCTQMKDNLPCGKCNNCQRIEKKEHPDVLVIEPEGQTIKVDQIRRLQTEF
                     SRSGYESRKKVFLIKEAEKMNSSAANSLLKFLEEPPGDFLAVLETDAVGRILPTIQSR
                     CQVLHFQELSKEALIHRLQEEQIPLEKAKLLAFLTNSLGKAVEISQDEWFNDAKDLIY
                     QWFVYLQKQDTQAFIYVQKKLVKSFKEKTQQFTGLSVLMFYYQEAQRQAVANGEYGKV
                     KQINQIIERILIAEQKLRSNVSFQAVAEQFVLHTIF"
     gene            8423..9247
                     /locus_tag="IWO89_001604"
     CDS             8423..9247
                     /locus_tag="IWO89_001604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage 0 sporulation family protein"
                     /protein_id="HAQ0456352.1"
                     /translation="MVEVVGVRYRDAGHIYYFAPGDSEYVYSDKVLVESQQSLHIATV
                     VLPKKEIDPNDLPEAIRPILHKASQAELEKDKKNKKDAYDALKIAKEKVQLRELEMKM
                     VKAEYTFDRNKLIFSFTADGRIDFRELVKDLAAVFRTRIELRQIGVRDEAKIIGGIGP
                     CGRPLCCSSFLGDFVPVSIKMAKDQSLSLNPTKISGLCGRLMCCLQYESEEYETAKKE
                     LPDFGKVIATPDGKGKVVGLNLLSRIVSIRLTGRENPVEYEWDELKEFIQVGEMNG"
     gene            9240..9587
                     /locus_tag="IWO89_001605"
     CDS             9240..9587
                     /locus_tag="IWO89_001605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication initiation control protein YabA"
                     /protein_id="HAQ0456353.1"
                     /translation="MDKRSLYDGLNQLESELRNTLTELVEMKHALHEIVEKNTTLEME
                     NQRLREHLRELNQVAQAPAENVKQELSKSRMNLEKIYEEGFHVCYDLYGSRRDNDEPC
                     AFCLEVIYRESGR"
     gene            9656..10528
                     /gene="rsmI"
                     /locus_tag="IWO89_001606"
     CDS             9656..10528
                     /gene="rsmI"
                     /locus_tag="IWO89_001606"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HAQ0456354.1"
                     /translation="MYNQKSFKGNPVYGDLYLVPTPIGNLEDMSYRSVRMLQESDLIA
                     SEDTRNTQKLLNHFEIHTPQKSLHEHNYKERVPQLIALLKEGKTIAQVSDAGMPSISD
                     PGHELVLACIKEGISVISIPGPTAGMTALIASGLVPQPFLFYGFLPRKKKEQKETLKK
                     LKEQTVTLIFYESPYRITATLTNFLEIFGNRQVVLCRELTKVHEEYLRGSTEELLAYI
                     EEHPVKGECCLLVEGNLFEMPVTEVDEELGTLKEQVEAKIASGEKPNAAIKAVALKNG
                     LKKQEVYRQYHELD"
     gene            10637..11758
                     /gene="dinB"
                     /locus_tag="IWO89_001607"
     CDS             10637..11758
                     /gene="dinB"
                     /locus_tag="IWO89_001607"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HAQ0456355.1"
                     /translation="MMELQFPLHNDTSRKIIHIDMDAFFASVEERDHPEFRGHPLVIA
                     RHPSDTGGRGVVTTANYEARKFGIHSAMSAQKAYELCPQAIFKPGDHHKYSDISKQVR
                     EVFYRYTDIVEPVSIDEAYLDVTENKINCKSAIKIARMIQADIWESVQLTCSAGVSYN
                     KFLAKLASDYQKPRGITVVAPQDAVPFLKALPIEKFHGVGKKTVPRMHELGIYTGKDL
                     YECTEMMLIRNFGKMGYSLYRKVRGIHDSPVNVTRERKSVGKEHTYGQPLQTEEAVLT
                     QLRQLAEKVEEALRRVQKHGKTVVLKVRYTDYSTVTKRVTLPEYIYKKEALFYQASLI
                     WEEILGVEKGIRLLGITLTNLDPMTYENIVLPLWENQEI"
     gene            complement(11812..12414)
                     /gene="nrdG"
                     /locus_tag="IWO89_001608"
     CDS             complement(11812..12414)
                     /gene="nrdG"
                     /locus_tag="IWO89_001608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase
                     activating protein"
                     /protein_id="HAQ0456356.1"
                     /translation="MRNPKPKEWLAKDYSQNYIADYKPFNFVDGEGVRNSLYVSGCLF
                     ACEGCFNKAAQNFRYGKPFTPELEEQIIADLRNDYVQGLTLLGGEPFLNTDVCLQVVR
                     RIRKEFGTTKDIWSWTGYAFDELLQDSEDKLELLSQIDILVDGRFELSKRDLKLQFRG
                     SSNQRIIDVQKSLESNQVVIWEKCTDATETYEQIKKQDLI"
     gene            complement(12729..14918)
                     /gene="nrdD"
                     /locus_tag="IWO89_001609"
     CDS             complement(12729..14918)
                     /gene="nrdD"
                     /locus_tag="IWO89_001609"
                     /EC_number="1.17.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase"
                     /protein_id="HAQ0456357.1"
                     /translation="MKVNYEEKTVVTQQPETSQMKVIKRDGRHVDFDEQKINEALIKA
                     EKKIHGSLSPLTYEKIQMIADLVVQEIKSRFAENVKIYEIQNIVEHILLEKNEYELAE
                     EYINYRTKRDFARSKATDINFSIEKLINKDQTVVNENANKDSNVFNTQRDLTAGIVGK
                     SIGLKMLPPHIANAHQKGDIHYHDLDYHPYTPMTNCCLIDFKGMLNNGFKIGNAEVES
                     PKSIQTATAQISQIIANVASSQYGGCSADRTDELLAPFAELNYKKHLKDAEEWIDSPE
                     RQKEYAKAKTKKDIFDAMQSLEYEINTLFTSNGQTPFTSLGFGLGENWFEREIQKAIL
                     QIRINGLGSEKRTAIFPKLIFTLKKGVNLNPEDPNYDIKQLALECATKRMYPDILNYD
                     KIVELTGSFKVPMGCRSFLQGWKDENGQEVNVGRMNLGVVTLNLPRIAIESKGDQNKF
                     WQLLSDRLEIMKDALLYRVERCKEAIPANAPILYMYGSFGKRLSRTDSVNELFKNRRA
                     TVSLGYIGLYEVASAFFGGEWETNPEAKAFTLDIVKELKANADAWGDEYGYHFSVYST
                     PSESLTDRFCRLDTEKFGIIENITDKEYYTNSFHYDVRKNPTPFEKLEFEKDYPKYCS
                     GGFIHYCEYPMLQQNPKALEAVWDFAYDKIGYLGTNTPIDHCYQCGFEGDFKPTERGF
                     ECPECGNHDPKTCDVVKRTCGYLGNPQARPMVHGRHKEISSRVKHMK"
     gene            15244..16011
                     /locus_tag="IWO89_001610"
     CDS             15244..16011
                     /locus_tag="IWO89_001610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ0456358.1"
                     /translation="MEKMIEVKHLSKSYGSKLSPVKALDNISFTVEEGEFVGIMGPSG
                     AGKTTLMNILSTINLPTMGSVSIQGKEITKMKNSELSDFRRKKLGFIFQEFNLIDTLN
                     AKDNILLPLAVERMTKEEMEKRVRHVAQLLNIEELLKRYPDELSVGQRQRIAAARALV
                     VQPQVIFADEPTGSLDSKSATELLNYLKTMNQKEKATILLVTHDPYTASYCDRILFIK
                     DGVIFSEVVRRGTRREFFEKVIDMQATIGGGGKMNAV"
     gene            16001..18043
                     /locus_tag="IWO89_001611"
     CDS             16001..18043
                     /locus_tag="IWO89_001611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAQ0456359.1"
                     /translation="MLYKLAVKSFFRQSRGYLVYFFSLTLSVMIYYSFSAMTYDQSLV
                     RRASQDTSIDGGLSFGGFIITVVLLFFVFSANRFFLNRRQKEIGIYQLFGMNKLQISG
                     IYVLEIMIIGLFACISGILLGIIFSKLFSMILVRMMDMDLTSPFFISIPSVVDTLFAF
                     FIILLAVSVYSIWKIWRYPMIRSFGEKEQADSSTMRFRTRHRLLAVIGLLLLTSGYFG
                     ASHFREITSWMISYHYLDTLVFFFPFLIFFVCVVGTYLFFCYTFRLFLIILSKWKIYY
                     YGINFLIMGNTQVHLLKSWRTNSLITVILGISLMTIGGTASISTILSYQEKVSSPMDY
                     QFDVETEKKIKPILAEEGQKITKEITLHYKVIGSIYSQNLEEPMFQTANLITETQYQA
                     FRKVNPDLPNISLKSPGHTVLLDQMQTLFRNISLYGSSIGLPDNQELKIQQMLPSVLG
                     DETMVYLSPVLIVDDTVFEKAKGVDYQVVNVDVSGGNNEKIDERLSNDLSIKWLNAVY
                     YSYDIVNGSLQGEISTKKLPDDQMKEAQFSQESSRLNFSSSYSKLRAVNRRIGINIFV
                     TLFVGMIVIIATGSILTVRQLAEAEEEKENYHLLTKLGIPQRRIRRMIFEQNALTFFP
                     PMILGITHAVFAINVFTQYIKTADYWLAYLVSGLLILIYLLLYVITSQLYCRIIEE"
     gene            18324..18473
                     /locus_tag="IWO89_001612"
     CDS             18324..18473
                     /locus_tag="IWO89_001612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="teichoic acid D-Ala incorporation-associated
                     protein DltX"
                     /protein_id="HAQ0456360.1"
                     /translation="MNKWRSNPKLNYWGKFIIRTIFYSAVLLFLIYLYHYKNIQGGTF
                     IYNEF"
     gene            18484..19998
                     /gene="dltA"
                     /locus_tag="IWO89_001613"
     CDS             18484..19998
                     /gene="dltA"
                     /locus_tag="IWO89_001613"
                     /EC_number="6.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--poly(phosphoribitol) ligase subunit
                     DltA"
                     /protein_id="HAQ0456361.1"
                     /translation="MEIKTIIEAIDDWGINEPDRVAYQADETHTFGELKQASDALAYY
                     LEKKVEGDGPIVVFGNLEFEMIVSFLGVVKSGHAYLPIEEHTPKERILSIFRVAKPSM
                     VISIGDWIEDIPSVPVITKKEFQEIKKIPVGFYPRNSVKGTENFYIIFTSGTTGEPKG
                     VQISHDNLVSFVQWTMEDFGIKPGMHFLAQAPFSFDLSVFSIYPALVSGGMLKPLNKA
                     VVQDFRQLFATLPALKLNVWVSTPSFMDICLMEPTFNGENVPELDMFLFCGEELTKKT
                     AESLLDRFPNARIYNTYGPTEATVAISSIQIDQHVLDSYDRLPIGYVKKDTKVSIVQE
                     GKPVSKGETGEIIIAGPSVSKGYLNNPEKTAAAFFCQNDLASYHTGDAGRLDEYGLLF
                     YEGRMDQQVKLHGYRIELGDIEHYLLQDNRIKQAVVVPKYQGTKVQQLVAFVVLENKT
                     KQPDFQLTKSIKEQLLEVVMDYMIPQKFHYVDLLPQTVNGKIDRKKLTAEVNPE"
     gene            19995..21200
                     /gene="dltB"
                     /locus_tag="IWO89_001614"
     CDS             19995..21200
                     /gene="dltB"
                     /locus_tag="IWO89_001614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-lipoteichoic acid biosynthesis protein
                     DltB"
                     /protein_id="HAQ0456362.1"
                     /translation="MMLPHMIPYAEPFYFFLLAIALVPIVLSLLIWEKRLPVYQSAVT
                     LFFLFVSFGGNYWQQGAALIGYVLWQTILVWGYFVYRQRKNQTGWFFAAVILAIIPLF
                     LTKVTPFFEHGKASLLGFLGISYLTFKSVQMIMEIRDGMIKTYKPFRYIQFLLFFPTI
                     SSGPIDRYRRFEKDQLHPPVRDKYVDLLGKGVQNIFVGFLYKFIIGYYFGQVLLPVVG
                     RIALQHGGISWALVGYMYVYSMYLFFDFAGYSLFAVGTSYMMGYQTPINFNKPFLSWN
                     IKEFWNRWHMTLSFWFRDYVYMRLMFFMMKKKFFKSKIVMSNIGYFALFLLMGIWHGL
                     TWYYIVYGLYHACLICLTDAWIRFKKKHKEQLPSNKFTHGLAVFLTFQAVCFSFLIFS
                     GFLDKLFFS"
     gene            21245..21478
                     /gene="dltC"
                     /locus_tag="IWO89_001615"
     CDS             21245..21478
                     /gene="dltC"
                     /locus_tag="IWO89_001615"
                     /EC_number="6.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--poly(phosphoribitol) ligase subunit
                     DltC"
                     /protein_id="HAQ0456363.1"
                     /translation="MEEQILSILEEITGTDEVRKNRDIDLFDEGLLDSLASVQLLVEL
                     DGELDIQVPVSEFEREDWSTPNKIIQQATALKG"
     gene            21481..>22342
                     /gene="dltD"
                     /locus_tag="IWO89_001616"
     CDS             21481..>22342
                     /gene="dltD"
                     /locus_tag="IWO89_001616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-lipoteichoic acid biosynthesis protein
                     DltD"
                     /protein_id="HAQ0456364.1"
                     /translation="MKKKLFAIFGPVVLAVGLLFVFFTSSYQVDISNPETIRRASSSM
                     SVNVLKGDAIKNYALSEKQYIPFFGSSELSRISPFHPSVLAEKYQRNYRPFLLGAPGT
                     QSLSQYMMMRSAGDAMKNKKVVFIISPQWFVKNGVKTDYFNTYYSELQTYDWLFSMKK
                     VTPADRYLARRLLTFSKVKENDTLTAILQTIKKGKLPLPESLNQLRSQWNMLKREDEV
                     FSNIGLKDRQQKIDHESKRLPKQYQETELSILANQIGERETTNNPFGLKNDFYTHRIR
                     AHEPELKQSQK"
BASE COUNT         6986 a         4022 c         4627 g         6707 t
ORIGIN      
        1 agttggtagg agaacaccgt ttaccaagag gttgtgaaaa agctgttctg catcaagtaa
       61 taagaagagc caagtaaaaa cagtctctta tgtttttaac ttggctggac ttatcaccgt
      121 agatgtaagc ttttgaacgc cgtttatttg caacaaaagg ggctgtgaca cgatttttgt
      181 cacagccctt tttttgcgaa cattcattta gcaattttta gaaacttctg ccgccaccgc
      241 caaaagtccc gccaccgcta gaatgggtag tacttccgcc accgccgctt ccaggaggtg
      301 ggcttttggg gattcttctt gtcgtgacaa aagaatttgt cagacgatcg gttttttcag
      361 ttaatttgat actggatttt tctcggaatg gatatttgta gatgcctgat ttcaattgat
      421 aacgcgaaac agtcacaaaa taaaagacaa gactaaggat aagagccaaa acaacagcaa
      481 tgatgatttc tactgtcgtc aaaaccttat agcgtgtaat tttgccagtt tcttcgtcta
      541 cacgataatg tcctcctgga actccgtctt ccacatatgt tgctgattta gacaggtaag
      601 cttttgcagc attgtaatag gcagcatttg tcatctgatc ataaacatca tctaagatcg
      661 aatcgattct gctatcagtc aaataatcga tcatattacc agaagtcgaa atgtagattt
      721 cacgctgtcc catatctaat agcaatactg agccattttg atcatttccg actgcgtctc
      781 ttaagtaatt atcagcaaag tctcttggtt cttctgaatt agatgtagtc gttacgatca
      841 agactcgtcc tttgattttt tcattcagct tttctgcctg ttgttccaaa gattggattt
      901 gatctgttgt gaataaccca gcctcatcat ttaccgtagc tgtatctgca tgagtagcca
      961 ccggccagcc aacacttact agcaaaagaa agaataaaaa aaactttttc atcatatcaa
     1021 gtaacctccg atcagaaaga ggatcgctag cccagcgaaa atcccaagag cggtcagtcc
     1081 caattttttc tggctgatcg gcaggacacc gctgaccttt cctgtttgtc cattcattgc
     1141 gtagtagtag acttttttac ttgagtcatt gcttcgataa gtaacaagcc ataccggcag
     1201 taatacgtaa ttatttactt cgtttgtgat tgaagcagaa gttcgcacac cggtcaatgt
     1261 tgtatatcca tttgcggtat cttttaacag cttctctgaa tagtctttta attcagattg
     1321 aatctgtgat ttgattgcct catattcgat atctcttttt tctgcttgga acccggctaa
     1381 atactggctt ttaaaatcaa ttgctttatc taacgggaaa ggttgcacgc cttcgaccat
     1441 cttttgttgc gtattttttg ataaggcatt ttttactagt tctttaaaag aaagcttgcc
     1501 tgctcttgaa acagcaaatt gcttcgtttc tgtgtattcg atgtcaccta tccgccatat
     1561 gcggataact gtaccattcg cctgcatggc accatctact tcagcatctg tagcccaata
     1621 agggaaatac acacctgtca acttatcgat ttgatcctta ttgaaaaaag ctttgggaac
     1681 aaaccatttt ttcttggtcc aagcgagaaa tctgttgatt gcttcttctt tctcgacagc
     1741 aaaaggaagt actttatttg gcaagaattc tccgcttaaa cgtcccgata gtacgacggg
     1801 attatggcag tagtaacaat aagtcgcagc agttgtcgca tcagtcacga tctctgcacc
     1861 acagttggga caaagaaaaa gatctatcgc tgcggtatta tctgcagatt cgtccgctgt
     1921 atctgaaaaa ccgcctgcct cgtcaaccgc tttgcgctcg gcttcattca tatcggcaag
     1981 ctgttcttgg gctgtaaaag taaaatcttc ggtaggtgaa ggttcttctt tgctttcttc
     2041 gccagccata cgcgcttctt tttgcttttg ctcatattgg ctgacttctt cttctgtata
     2101 gatattcagg cagtattcgc aatgaaattt ttggtctttg ggatcaaaag tcaacggacc
     2161 gccgcaatta ggacatttat gtgtaagaac atccatcagc tgcacacctt tctaaataaa
     2221 ttaattaacg gggattcctt taaacggttt accacagttt ggacagaatt tagggatgcc
     2281 gttggaaaga tccacgatct cattgcattc actgcatcgc atttgtagct taggctgaac
     2341 gttttctaca ggtttggcag ctccgcagtt ggaacagaat tttccggaat tttctgtccc
     2401 gcaaactgga catgtccaag tatccgcatt tccttgtgca gtttgttgat tttgtttttc
     2461 tgcttgttgt ttgatttgtt cttggttatt ttgagctgct tgagaaagat aagaaccatt
     2521 tgcattcatt cccataccta cacccataaa gccggtcatt gctcctgctt cattttttcc
     2581 ggctgcttcc ataccacgtg caatggaacc ttgcacgtat ccttcacgta cgctaggatc
     2641 acctagcatc gccccttcgt tacgcatatt gatcagtttc acagaatcat cggtgtaaga
     2701 aatgctggct acagccacag aaacaatctc catcccacgt aattcacgcc aagaatcatc
     2761 caatgcagta tccatgtatt tgcttaattc caaactctta gatggtacat aagaaatccg
     2821 ttgaccatct gcagacatct gattgattgc tgattgtagc gctgttagga attcagctaa
     2881 atactgctca ttgatatcat tgatttccac ttgtgttttg tttttaggga ttgcgtttgt
     2941 ataaaataaa atcgggtcaa cgacatgaat agaataagtg ccatgtgccc gtaagaataa
     3001 ttctgcgtta tagaaattgt caaagtagtt caacggcgcg cttgtaccga atttgattcc
     3061 ctttatttct tggagattga tataaaaaac ttgttgtttc tgcggtgtga cgccgccaaa
     3121 tttgaatcgg tcaaaagttt cagcaattgc ttctttcagt gttccattga acatggaagg
     3181 agctgcgtca tttttaatgg tgtaataacc ttcctcagcg gtataatcga tgatttttcc
     3241 accatcgacc aaaagcatca tcatgtttgg atagacatgg acgactgttc cgtctgttaa
     3301 aacatcttct gttcctttac gattggagcc acgtttgtca tctttgcgga ctttgacacc
     3361 ttttgtcatg accgttgtat cactcatatt gtctggttcg atgacttcta gccattgatc
     3421 tgccagaccg ccgccaacca tacttgttgc tgctttgata agacccataa aaaattcctc
     3481 ctctgattca gctagtgcca aacaattatt gtttatttat tataccatag gaagatgtga
     3541 aacttctcgg actagagacg gaattgaaat ggaaaattgc gtattgttta aatacctgaa
     3601 aaagccgaga aggatagtag tttttttacc taatatcacg tataataaga gatagtgaat
     3661 tttttaaaaa agggggatcc ttatggcata tcaagcactt tatcgagtct ggcgttcgca
     3721 gcgtttcgaa gacatcgttg gacaaaaagc tgtgacacag acactaaaaa atgctattgt
     3781 ttcccacaaa acctcacatg cctacttgtt tacaggaccc agaggaaccg gaaagacgag
     3841 tgcagcaaag attttcgcaa aagcaatcaa ctgtccgaac agtaaagacg gtgagccatg
     3901 caacgaatgc gaaatgtgtc gatcaattac tgctggtacg caagaagatg tgatcgaaat
     3961 cgatgctgcc agcaataacg gagtcgaaga aatccgattt atccgtgatc gggcaaatta
     4021 tgcgcctaca aaggcagcgt acaaagtgta cattattgat gaggtacata tgctttcaac
     4081 aggagctttc aatgccttgt tgaaaacatt ggaagagcca aaagaaagtg tcatattcat
     4141 cttagcaacg accgaacctc acaaaatacc agctacgatc atttcacgga ctcagcgatt
     4201 tgattttaaa cggatcaata cggcagatat tgtcgaacat cttgaattta tcttgaaaga
     4261 atcgactatc agctatgaac cagctgcatt gaatgtgatt gcccgttctg cagaaggcgg
     4321 gatgcgggat gcattgagta ttcttgatca ggcaatctcg tttagtgaag gaacaattac
     4381 tttagatgat gccatgcaag tgacaggtag cttaactgac gaaatgatgg acagttactt
     4441 gagtgcttgc gtgaaaaaag aagtaccaga agcattggaa acattgaacc aaattctcgc
     4501 agccggcaaa gaatcacgtc gctttctaga ggatatgtta caatactgcc gtgacttgct
     4561 gatgtatcag caagcaccta acctagttga ggaacaatcg agtacgatca gaagtacatt
     4621 tacagaattg gcacaaactg tttcagcgga tcagctgttt tcaatgataa agattttgag
     4681 cgatactcaa aatgaaattc gttttaccaa tagtgccgct atttatttgg aagtcgctac
     4741 agtcaaactt gcacagctac cgtctgcttc tgttccttca gcagatgtag catttgcaga
     4801 tgatcagata gtaaaacagc tacaacaaga gattaaggaa ctaagaaacg aaatcaagga
     4861 actacgcagt aatacaggaa ttgagaatac aaaagtacag ccggtacagc aaacaaatca
     4921 taaaaaacag acaagtacct atcgtgtacc gacggaacga gtatataaag ttctgcggga
     4981 agcgacaaga cagcatctga accaagtaaa agaagtatgg gaagacttgc tgatgagtct
     5041 atctgtcact cagcgagcaa tgcttaaagc aagcgaacca gttgcagctg gacctacagg
     5101 actagtgatt gcgtttgatt atgagattgt ttgtcaaaga gcagcaaatg acgaagaatt
     5161 gcagctttcg gtacacaaca acttaagccg aatgatcaaa gattatgcgc ctgatcctgt
     5221 ttatattaca agagaaagct ggccagaact tcgaagaaat tatttgacgc aggcacaaga
     5281 agagggaaca gaaaactctt ttgcttctga agactcagaa ggagaaatcg agttgcttcc
     5341 tcaagaacaa gaagtaccag aagaaacact tgtagtaaaa gcagaggaac tgtttggctc
     5401 cgaagctgtt acagtggtag atgattaata aaaaggagta gataattatg atgcgtggaa
     5461 tgggcaatat gcaaggcatg atgaaacaag tacaaaaaat gcaaaaagaa atggcagaag
     5521 cacaagagct gctaaataaa acagagttta caggtaaagc caccaatgaa ttggtcaaag
     5581 cggtttttac aggcgatcgt cgcatgaaag atatccaaat caatgaagaa gtcgttgatc
     5641 ctgatgatat cgagatgctt caggatcttg tcgtaatggc agtcaatgat gcgatcgcac
     5701 aaattgatgt ggaaactgag aaaaaattag ggaaatacgc aaaaggtttg ccaggatttt
     5761 agttttaaac tgaaggagta atgaatatgc aatatccaga accaattgcg aaactgatcg
     5821 acagctacat gaaacttcca ggaatcggtc agaaaacagc gacaagactc gccttttata
     5881 cgatcgatat gaaagatgaa gtagtaaatg aatttgctaa atctttgtta agtgtaaaac
     5941 gcgatttgca tttttgtagt atctgcggaa acatcacaga agaagatccc tgcgaaatat
     6001 gtagaaatcc ttcacgtgat cgaagcgtta tcctagtggt cgaagagccg aaagatgtaa
     6061 tggcaatgga aaaaatgcga gaatatcacg gactttatca tgttctccat ggtgtgttat
     6121 caccgatgga agggacaggt ccagaagaca tcaatatttc ttctctgttg cagcggctgc
     6181 atgatgcaga tgtaaaagaa gtgattatcg caacaaatgc aacaacagag ggagaagcaa
     6241 cagctatgta tctctcacgt ctgatcaaac ccgcaggtat caaagtgact cgtctagcac
     6301 atggtctgtc tgtaggctct gatatcgagt atgcagatga agttacttta ttgaaagctg
     6361 tcgaaggaag acgtgagtta taaatttcta attaagtgtt ttttcagata caatataaaa
     6421 gcagcacaag agatgggggc aacaatgtga acggactatt tattacaatc gaggggcctg
     6481 atggtgcagg aaaaaccagc atcttgaatg aactttaccc tcttttgaaa gaagtagcaa
     6541 aaacagaaat cattcaaaca agagaaccag gaggcattcc gattgctgaa gagatccgag
     6601 cagtgatcct tgatccaaaa aatgatcgga tggatgaacg tacggaagca ctgctttatg
     6661 cagcagcaag aagacaacat ctagtcgaaa aggttctgcc tgctctggca aaagggaaaa
     6721 tcgttttatg cgatagattt gtcgacagtt ctcttgctta tcaaggtgca ggccgacgca
     6781 tcggcgttac cgagattgct cggttgaatg agtttgctac agaaggtact acgccggatt
     6841 ttacccttta tcttgacgta gattcagaca ccggattaag aagaatcaag aaaaacagac
     6901 aaaatcaaat cgaccgttta gattcggaag gtttggaatt ccaccagcgt gtacgtcatg
     6961 cttacttaaa gctggcagaa gagaatccgg aaagaatcca caaagttgat gcaagaaaaa
     7021 gctttgaaga agtgctgcaa acaagttatc atacaattat tgaacagtat ccgcaatttt
     7081 ttgaaaacta ggaaggtgaa cgtttatgaa aattatttta gcgattatcc aagacaaaga
     7141 cagcaaccgt ttatcaaatg aattgattga tgcaaatatc cgtgccacaa aactatcatc
     7201 gacaggcggc ttccttaagg ctggaaacag tacgtttatc gtgggtattg aagatgaccg
     7261 tgtagaagaa gcattggaaa tcatcaaaaa aacgtgtgaa tcaagaaagc aatttgtatc
     7321 tacacctgtt acactggata tttcgatgga tgggggcgtc ccttatccag tagaagtaga
     7381 agtaggcggt gccacagtct tcgtcttacc tgttgaagga ttccatcaat tttaggagga
     7441 gctgagctat gaatcaagaa ataaacttgg ctcaaatgca accgatcgtg tatcagcagc
     7501 ttcaacggag ttttgagcat gggcgtatcg ctcatgctta tctttttgaa ggggagaaag
     7561 gaacaggaaa acatgaagta ggtatatggc ttgcacagca cctgttctgt acccaaatga
     7621 aagacaatct gccttgtgga aaatgcaaca attgtcagcg gatcgaaaag aaagaacatc
     7681 cagatgtttt ggttattgaa ccggaaggac aaaccatcaa agtcgatcag atcagacgtt
     7741 tgcagacgga attcagtcgc agcggttacg aatcaagaaa aaaagttttt ttgatcaaag
     7801 aagctgaaaa aatgaatagc agtgcggcaa acagtttgtt gaaattcttg gaagagcccc
     7861 ctggagattt tttagcggtg ttagaaacag atgcagtagg aagaattctc cctactattc
     7921 aatctcgttg tcaagttctg cattttcaag aactttcgaa agaagcactg atccatcgat
     7981 tgcaagaaga gcagattcca ttggaaaaag caaaactttt ggctttctta acaaacagtt
     8041 taggaaaagc agttgaaatc tctcaagatg aatggtttaa tgatgctaag gacttgattt
     8101 atcaatggtt cgtttacttg caaaagcaag atactcaagc ctttatctat gtccaaaaaa
     8161 aattggtaaa atcatttaaa gaaaaaacac aacagtttac aggattatct gtcttgatgt
     8221 tttattatca ggaagcacag cgtcaagcag tagcaaacgg cgaatatggc aaagtgaaac
     8281 agataaacca gataattgaa cggatattga tcgcagagca gaaactacgc agcaatgtca
     8341 gttttcaggc tgttgcggaa cagttcgttt tgcatacgat tttttagctt gccgcaaagg
     8401 atgatgatca aggaggatgg aaatggtaga agtagtaggt gtccgatata gagatgcagg
     8461 tcatatctat tattttgcac caggagactc tgaatatgtt tatagtgaca aggtattagt
     8521 cgaatctcag caatccttac atattgctac tgttgtcctt cctaaaaaag agattgatcc
     8581 caatgacctt cctgaagcta tccgtccgat cttgcataaa gcgtcacaag ctgaattaga
     8641 aaaagacaaa aaaaacaaaa aagatgctta tgatgcattg aaaatcgcaa aagaaaaagt
     8701 gcagttgcgt gagttggaaa tgaaaatggt caaagccgaa tacacatttg atcgaaataa
     8761 attgattttt tctttcacag cggacgggcg aattgacttt agagaattgg taaaagatct
     8821 ggccgctgta tttcgtactc gtattgagct tcgacaaatt ggtgtccgcg atgaagcaaa
     8881 gataataggc ggaattggtc catgtgggcg tccattatgc tgttcttctt ttttaggcga
     8941 tttcgtgcct gtatcaatca aaatggcaaa agatcaaagt ttatctttga atccaacaaa
     9001 aatttccggt ttatgcggac gcttgatgtg ctgtctgcaa tatgagagcg aagaatacga
     9061 gacagcgaaa aaagaactcc ctgactttgg aaaagtaatt gctacacctg atggtaaagg
     9121 gaaagtcgta gggttgaatc tgcttagccg tatcgttagt attcggctga ctggacggga
     9181 aaatcctgtc gaatatgaat gggatgaatt gaaagaattt atccaagtag gtgagatgaa
     9241 tggataagag atcattatac gacggattga atcaattaga atcagagttg agaaatacat
     9301 taacagaact tgttgaaatg aagcatgctt tgcatgagat cgtagaaaaa aatacaactt
     9361 tggagatgga aaatcaacgt ctgcgggaac acctgcggga attgaaccaa gttgcacaag
     9421 ctccagctga aaatgtgaag caggaacttt ctaagtctcg tatgaatctt gaaaaaattt
     9481 atgaagaagg attccatgtc tgctacgatt tatatggatc aagaagggac aatgacgagc
     9541 cttgtgcgtt ttgtctagaa gtgatctatc gcgaaagcgg tcgatgacca ataacaagag
     9601 gagactggag cggagaaata ctgctccagt ctttttatta aggagattgt gacagatgta
     9661 taatcaaaaa agtttcaagg gaaatccggt atatggggat ttatatcttg taccgacgcc
     9721 aattggaaat ttagaagata tgagctacag aagtgttcgt atgctgcaag aatccgatct
     9781 tatcgcaagt gaggatacgc gtaatacaca aaagttattg aatcattttg aaatccatac
     9841 accccaaaaa agtttacatg aacataatta taaagaacgt gtccctcaat tgattgcttt
     9901 gttaaaagaa ggaaaaacga ttgctcaagt cagtgacgca ggaatgcctt ccatcagcga
     9961 tcctggacat gagctagttt tagcctgcat aaaagaaggg atttctgtga tctctatccc
    10021 aggacctaca gctgggatga cggctttgat tgcttcaggg ctagtgcctc aaccattttt
    10081 attttatggc ttccttccac gtaagaaaaa agaacagaaa gaaacattga aaaagctaaa
    10141 agaacagaca gttacgctga ttttttatga gtcaccttat cggatcactg ctacattgac
    10201 gaatttttta gaaatttttg gaaatcgcca agtcgttttg tgtcgggaat tgacaaaggt
    10261 ccatgaagaa taccttagag gaagtacgga agagctactc gcttatatcg aagaacatcc
    10321 agtaaagggt gaatgttgtc tgcttgtaga agggaatttg ttcgaaatgc cagtgacgga
    10381 agttgatgaa gagttgggaa cgttaaagga acaagtagaa gcaaagatcg cttcaggcga
    10441 aaaaccaaat gctgctatca aagcagtagc gctgaaaaat gggctgaaaa aacaagaagt
    10501 ctaccgtcag taccatgaat tagactgaga tagtataagt tgtctataat gaaagactaa
    10561 tgaggaaatc agctactaac ttttcaaatc tcttgaacgc gaacgaatgt tcttgtataa
    10621 tgaataagag gtgaggatga tggagctgca atttccttta cataatgata catcaagaaa
    10681 aatcattcat attgatatgg atgctttttt cgcttcggtt gaagaacgag atcatcctga
    10741 gtttcgcgga catccattag taattgccag acaccccagt gacactggtg ggcgtggagt
    10801 agtgactaca gcaaattatg aagcaagaaa attcggaatt cattcagcaa tgagtgcaca
    10861 aaaagcgtat gagctttgtc cgcaagccat ctttaagcct ggcgaccatc ataaatacag
    10921 cgatatttcc aaacaagtac gggaagtatt ctatcgttat acagatattg tggaaccagt
    10981 tagtatcgat gaagcgtatc ttgatgtgac cgaaaataaa atcaattgca agtcagccat
    11041 caaaatcgcc cgaatgatcc aagcagatat ttgggagagt gtccagctaa catgttctgc
    11101 gggagtaagt tacaataagt ttttggccaa attagcatca gactatcaaa aacctagagg
    11161 aatcacggta gttgcccctc aagacgctgt gccattttta aaagctttgc caattgaaaa
    11221 atttcatggg gtagggaaga aaactgtacc tagaatgcat gagctaggga tttatacagg
    11281 aaaagaccta tatgaatgca cagaaatgat gttgattcga aattttggaa aaatgggcta
    11341 ttctctttat cgtaaagtca gaggaatcca tgattctcca gtaaacgtta caagagagcg
    11401 aaaatctgtg ggaaaagagc atacttatgg gcagccgttg caaacggaag aagcagtgct
    11461 gactcagttg cgccaactag ctgaaaaagt agaagaagca ctgagaagag tccaaaaaca
    11521 tgggaaaaca gtagttttga aggtcaggta cacggactat tctactgtga ccaaaagagt
    11581 gacattgcca gaatatatct ataaaaaaga agcactcttt tatcaagcaa gccttatatg
    11641 ggaggaaatc ttgggagtag aaaaaggaat ccgattgtta gggattactt tgacgaatct
    11701 ggacccgatg acttatgaaa acatcgtcct tccattatgg gaaaatcaag aaatataaaa
    11761 acagctttga gagttgaaac cggaaatccg ttttctctcc caaagctgtt tttatatcaa
    11821 gtcttgcttt ttgatttgtt cataggtttc tgttgcatcc gtacattttt cccaaatcac
    11881 aacttgattg gattctaaag atttttggac gtcaatgatc cgctggttgc tacttccgcg
    11941 aaattgtagt tttaggtcac gtttgcttaa ttcaaagcgt ccgtcaacaa ggatatcgat
    12001 ttgcgaaagg agttctaatt tatcttctga atcttgtagc aattcatcaa aggcatagcc
    12061 tgtccatgac cagatatctt ttgtcgtacc gaactctttt cggatacgtc gtacgacttg
    12121 cagacaaaca tctgtattca aaaaaggttc gccaccaagt aaagttaatc cttgcacgta
    12181 atcgttgcgc aagtcagcga tgatttgttc ttctaattca ggtgtgaatg gtttgccata
    12241 tcggaaattt tgtgcagctt tgttgaagca gccttcgcag gcaaataagc agccactaac
    12301 gtaaagactg ttgcggacac cttcaccatc gacaaaatta aacggtttgt agtcagcaat
    12361 atagttttgg ctgtagtctt ttgctaacca ttcttttggt tttggatttc tcataaaaaa
    12421 acttctttca tttaaggttc aaagatatag ctattgaaga taaaatctac tcgaccacat
    12481 aaataagaac aaactgacct aaatccattg ttcaagaaag cttgttctta ttttgataga
    12541 gggcgttcaa agatacaaag ccatcgaaag agggtggtga aaaaagactt gtcatagcct
    12601 cttattctga ataaacggtg ttcaaaagct tgcatctgca gtaataagcc caactaaacg
    12661 aaaaatatga ggaactattt ttgtttagct attcttatta cttgatgcag gacagctttt
    12721 tcacaacctc atttcatgtg tttcacacgt gaggaaattt ctttatggcg gccatgtacc
    12781 atcggacgag cttgcggatt tcctaaatag ccacaagtac gtttgactac atcacatgtt
    12841 tttggatcat gatttccaca ttctggacat tcgaaccctc gttctgtcgg tttgaaatca
    12901 ccttcgaaac cacattgata gcaatgatcg attggcgtat tcgtaccaag atacccgatt
    12961 ttgtcatatg caaaatccca tacagcttct aaagctttcg gattttgttg gagcataggg
    13021 tattcacagt agtggatgaa tccacctgaa caatattttg gataatcctt ctcaaattcc
    13081 aatttttcaa atggcgttgg gtttttacgc acatcatagt ggaaactgtt tgtataatat
    13141 tctttatctg tgatattttc gatgatgccg aatttttctg tatccaaacg gcagaaacgg
    13201 tcagttagac tttcacttgg agttgaatag acactgaaat gataaccata ttcatccccc
    13261 catgcatctg cattagcttt taattctttg acgatatcaa gtgtgaaagc ttttgcttct
    13321 gggtttgttt cccattcgcc accgaagaaa gctgaagcaa cttcatacaa tccaatgtag
    13381 cctaacgata cagtagctcg acggtttttg aataattcat tgacagaatc ggtacgtgaa
    13441 agacgtttcc cgaaagaacc atacatatat aaaattggcg catttgctgg aatggcttct
    13501 ttacaacgtt ctacacgata gagaagcgca tctttcatga tttctaagcg gtcagacagt
    13561 aactgccaga atttattctg atctcctttt gattcgatcg cgattcttgg caaattcaat
    13621 gtaacaacac ctaaattcat tcgtccgaca tttacttctt gaccattttc gtctttccac
    13681 ccttgcaaga atgaacgaca gcccataggt actttgaaac ttccagttaa ttcaacgatt
    13741 ttatcgtaat tcagaatatc aggatacatt cttttcgttg cacattccaa tgctagttgt
    13801 ttgatgtcgt aattaggatc ttcagggttt aagttcacac cttttttcaa agtgaagatc
    13861 agtttaggga agatagctgt ccgcttttca ctccctaatc cattgattcg aatctgtaaa
    13921 attgcttttt gaatttctct ttcaaaccag ttttctccta gtccaaatcc taatgaagtg
    13981 aaaggagttt gtccatttga agtaaataag gtattgattt catattccaa actttgcata
    14041 gcatcgaaga tatctttttt ggtttttgct tttgcatatt ctttttggcg ttctggcgaa
    14101 tcgatccatt cttccgcgtc ttttagatgt tttttatagt tcaattctgc aaaaggagca
    14161 agcaattcat ctgttcggtc ggcagaacag ccaccatatt gacttgacgc tacattggca
    14221 ataatctgtg agatttgtgc tgtggcggtc tggatagatt ttggcgattc gacttcggca
    14281 ttgccgattt tgaacccatt gttcagcatg cctttgaaat caatcaagca gcaattcgtc
    14341 atcggtgtat aaggatgata atccaaatca tggtaatgga tatcgccttt ttgatgcgca
    14401 tttgctatat gtggtggcaa cattttcagt ccgattgatt ttccaacgat cccggctgtc
    14461 agatctcgct gtgtattgaa aacattactg tctttatttg cgttttcgtt cacgacagtt
    14521 tggtccttgt tgattagctt ttcaatcgaa aagttgatat ctgttgcttt actgcgagca
    14581 aaatctcgtt tggtacgata attgatgtac tcttctgcta attcatattc gtttttttct
    14641 agtaatatat gttccacgat gttttgaatc tcatagattt tgacattttc agcaaaccgg
    14701 cttttgattt cttggacaac caagtctgca atcatttgaa ttttttcata agtaagtggt
    14761 gataatgagc cgtgaatttt cttttctgct ttgatcagtg cttcattgat cttttgttca
    14821 tcgaaatcaa catggcggcc atcgcgtttg atgactttca tctgggatgt ctctggctgc
    14881 tgagtaacga cagttttttc ttcataattt actttcatta tcttcaactc ctgctaaatt
    14941 gttcttgtct atcataaaac aaaatcgggg aaggagcaac taaatatcgg ctatatatag
    15001 tattcaatgt gctgtataat tggatttcta tactatatgt agtgtatatt ttattgtgaa
    15061 aaaaggtgaa aacttgtagc tggtggattt gttttttgca tatttaattt tgctgaataa
    15121 aataattgaa taaaattttt tattcagtcc aagtaaaagt gacaaaaaca taagagcaaa
    15181 ttattcgtag atatgctact atgcaaataa atagaaatga aaaatgaaag aggaggagaa
    15241 gaattggaaa agatgatcga agtgaaacat ctaagtaaaa gctacggcag caaactttct
    15301 cccgtcaaag ccttagataa tatctcattc acagtagaag aaggcgagtt tgttgggatt
    15361 atgggcccaa gtggtgcagg aaagacaacg ctgatgaata tcttgtccac gatcaatttg
    15421 cctacgatgg gcagtgtctc gatccaagga aaagaaatca caaaaatgaa aaacagtgag
    15481 ctcagtgact ttcgtcgaaa aaaactagga ttcatttttc aagagttcaa tttgatcgat
    15541 accctaaatg caaaagacaa tatcctattg cccctagcag ttgaacggat gactaaagag
    15601 gaaatggaaa agagagtaag acatgtagct caattgttga atatagaaga actgctcaaa
    15661 cggtatcccg atgaactttc tgtcggacag agacaacgaa ttgcagctgc tagagcactt
    15721 gttgttcagc cacaagtgat ttttgcagat gaaccaactg gctctttgga ctcgaagtcg
    15781 gctacagaat tgttgaacta cttgaagacc atgaatcaaa aagaaaaagc aacgatactg
    15841 ttagtcaccc atgatccata tacagcaagc tactgcgata gaattttatt tattaaagat
    15901 ggtgtgattt tttctgaagt tgtcagaaga gggacaagaa gagagttctt tgagaaagta
    15961 atagacatgc aggcgacgat tggcggagga ggaaaaatga atgctgtata aactagccgt
    16021 taaaagtttt tttcggcagt cacgaggcta tcttgtttat tttttcagtt tgactttatc
    16081 cgtaatgatc tattattcct tcagcgcaat gacctacgat caatctttag tcagaagagc
    16141 aagtcaagat acttcgatag atggtggatt gagttttggt ggatttatca ttactgttgt
    16201 tttgcttttt ttcgtattca gtgccaaccg atttttcttg aatagaaggc aaaaggaaat
    16261 aggcatctat cagctttttg gtatgaataa actgcagata tcaggtatat atgtgttaga
    16321 aatcatgata attggattgt tcgcctgtat ttctgggata ttactcggta ttattttttc
    16381 caaactcttt tcaatgattt tggttcgaat gatggatatg gatcttacta gtccattttt
    16441 tatctccatt ccatcagtag tagatacttt atttgctttt ttcatcatcc ttttagcagt
    16501 atctgtatat tcaatatgga aaatatggcg ctatccaatg attcgaagtt ttggagaaaa
    16561 agaacaagca gatagttcaa caatgcgctt caggacaagg caccgattac tcgcagtcat
    16621 cgggttacta cttttaacga gcggatattt tggagctagt cattttaggg aaatcacttc
    16681 atggatgatc agttatcatt atcttgatac attagttttc ttttttcctt ttttaatttt
    16741 tttcgtttgt gtggtcggta cttatttgtt tttctgttat acatttcggt tatttttgat
    16801 catattgagt aaatggaaga tttattacta tggtatcaat tttttgataa tgggtaacac
    16861 gcaagttcat ttgctcaaaa gttggcgtac aaattctctg attacagtca ttctcggaat
    16921 ttctttaatg acaattggcg gcacggctag tatctctact attttatcct accaggagaa
    16981 agtttcctcc ccgatggact atcagtttga tgttgagaca gagaaaaaaa tcaagccgat
    17041 tctagcagag gaagggcaga agataactaa agaaatcacc ttgcattata aagtcattgg
    17101 gagtatctat agtcaaaatc tcgaagaacc tatgtttcaa actgctaatc tgatcacgga
    17161 aacacaatat caagccttca gaaaagttaa tccagatttg ccgaacattt ctttgaaatc
    17221 acctggtcat actgttttgc ttgatcaaat gcaaactctt tttcggaata tttcattata
    17281 tggatctagt attggactgc cagataatca agaattgaag attcaacaaa tgcttccaag
    17341 tgtattaggt gacgaaacaa tggtatatct aagtcctgta ctgattgtag atgataccgt
    17401 ttttgaaaaa gcaaaaggtg tggattatca agtagtcaat gtagatgtat caggtggaaa
    17461 taatgaaaaa atagatgaac gactgagcaa tgacctgtcg ataaagtggc tcaatgctgt
    17521 ttattattct tatgatatcg taaatggaag tctacaagga gaaattagca caaagaaact
    17581 tccggatgac caaatgaagg aagcacaatt tagccaagaa agctctcgtt tgaatttctc
    17641 ttccagctat tcgaaattgc gtgcagttaa tcggcgaatc ggtatcaata tttttgtcac
    17701 actatttgtc gggatgatcg tgattattgc tactggtagt attttgacag tccggcaatt
    17761 agcagaagca gaggaagaaa aagaaaatta tcacctattg actaagctgg ggattccaca
    17821 aagaaggatc agacggatga tcttcgagca aaacgcactt accttctttc cacccatgat
    17881 attagggatc actcacgcag tatttgcaat caatgtcttc actcagtata taaaaactgc
    17941 cgattactgg ttggcttatc ttgtgagtgg cttattgatc cttatctatc ttttgcttta
    18001 tgtcattacg agtcagttat attgtcggat tatcgaggaa tgatatacgg aataagacaa
    18061 gataataaat agaactaaat tttgaatacc atttatttgg aataagaggt tgtgacacga
    18121 tttttgtcac aacctcttta ttttttatat tttttaaaag tacgagaaat tataaagaaa
    18181 aaagcagtga tttgatgtgc tatttttata aaagctatgt gacaaaactg ttagtaaaaa
    18241 aacggtttct gtaagctaga cacatgtttc tttttgtcaa aattagttac actttgtgtg
    18301 aggttatatg aggagggaat ccaatgaaca aatggcgttc taatccaaaa ttgaattatt
    18361 ggggaaaatt catcattcga acaatttttt atagtgcagt actgctgttc ctgatttatc
    18421 tgtatcacta taaaaatatt caaggaggaa cttttatcta taacgaattt taggaggaag
    18481 agtatggaaa tcaaaacgat tattgaagca atcgatgatt ggggaataaa tgaacctgat
    18541 cgagttgctt atcaggcgga tgagacgcat acttttggtg aattgaaaca agcatcagac
    18601 gcacttgctt attatttaga aaaaaaggta gaaggcgatg gaccaattgt cgtttttggc
    18661 aatttggagt ttgaaatgat tgtctcattt ctaggggttg tcaaatctgg ccatgcctat
    18721 ttgccgattg aagagcatac acccaaggag cgtatcctat cgatctttcg ggtggctaaa
    18781 ccatctatgg tcatcagtat cggggactgg atagaagata ttccttcggt tcctgtcata
    18841 acaaaaaaag aatttcaaga aatcaagaag ataccagtag gattttatcc cagaaatagc
    18901 gtaaaaggta cagaaaattt ttatattatt tttacttctg gtactactgg agaaccaaaa
    18961 ggcgtacaaa tcagtcatga taatctagtc agctttgttc aatggacgat ggaagacttt
    19021 gggatcaagc ctggtatgca tttcttagcc caagcgccat tttcttttga tttatcggta
    19081 ttttctattt atcctgcatt agtttctggc ggaatgctga agccgttaaa taaagcagtt
    19141 gttcaagact ttcgtcaatt attcgcgaca ttgccagcct tgaagttgaa tgtgtgggtt
    19201 tctactccat catttatgga tatttgcttg atggaaccta cattcaatgg agaaaatgtg
    19261 ccggaattgg acatgttttt attttgcgga gaagaattga cgaaaaaaac tgcagaaagt
    19321 ctacttgatc gattcccaaa tgcacggata tataatactt acggtccaac agaagcaact
    19381 gtagctattt caagtattca gattgaccag catgtactag atagttatga ccgactaccg
    19441 attggctacg taaaaaaaga cacgaaagtt tctattgtcc aagaaggtaa acctgtttca
    19501 aaaggagaaa cgggtgaaat cattattgca ggaccaagcg tatcaaaagg ttacctgaac
    19561 aatcccgaaa agacagctgc tgcgttcttt tgtcaaaatg acttagcaag ttaccataca
    19621 ggggatgcag gtagactaga tgaatatggg cttttatttt atgaaggacg tatggatcag
    19681 caggtcaaat tacatggata ccggattgaa ctcggggata tcgaacatta tctgctccaa
    19741 gacaaccgaa tcaaacaagc tgtcgtcgta ccaaaatacc aaggaacaaa agtacagcag
    19801 ctggttgctt ttgtcgtatt agaaaacaaa acgaaacagc cggattttca gttgactaaa
    19861 tctatcaaag aacaattact agaagttgta atggattata tgattcctca gaagttccat
    19921 tatgtcgatc tgctgcctca gacagttaat ggaaaaatcg atcgaaaaaa attaacagct
    19981 gaggtgaatc ctgaatgatg ttacctcata tgatccctta tgcagagccg ttttattttt
    20041 ttcttttagc cattgcgctt gtaccgatcg ttctatcttt attgatatgg gaaaaaagat
    20101 taccagttta tcaatcagca gtgacacttt tctttttatt tgtcagcttt ggaggtaatt
    20161 attggcaaca gggagcggcg ctgattgggt atgttttgtg gcagacgata cttgtatggg
    20221 gctattttgt ttatcggcaa aggaaaaatc agacaggatg gttttttgcc gccgttattt
    20281 tagcgataat tccactgttt ttaacgaaag tgactccttt ttttgaacat ggaaaagcat
    20341 cgttacttgg ttttttagga atctcttatt taacctttaa atctgtacaa atgatcatgg
    20401 aaataagaga tggcatgatc aaaacatata agccatttcg ttatatccag tttctgctgt
    20461 tctttccaac aatctcttct ggaccaatcg atcgctatag gagatttgaa aaagatcagc
    20521 tccatccgcc tgttcgtgat aaatatgttg atcttttggg aaaaggcgtc caaaatattt
    20581 ttgtcggttt tttatataaa ttcattatcg gttattattt tggtcaagta ctgcttcctg
    20641 tagtgggacg cattgcctta cagcatggtg gtatttcatg ggcattagta ggttatatgt
    20701 acgtgtacag tatgtatttg ttttttgatt ttgctggcta tagcttattt gcagtcggta
    20761 caagctacat gatgggatat cagacaccaa tcaatttcaa taaacctttt ttatcatgga
    20821 atatcaaaga attctggaat cgttggcata tgacgctgtc cttttggttc cgtgattatg
    20881 tatatatgcg attgatgttc ttcatgatga agaaaaaatt ttttaaaagc aagatcgtaa
    20941 tgtcaaatat cggttatttt gctttgtttc ttctaatggg tatatggcat ggattgacgt
    21001 ggtactacat cgtatatgga ctgtatcatg cttgtctgat ctgtctgaca gatgcatgga
    21061 tccgatttaa gaagaagcat aaagagcagt tgccttctaa caaatttacg catggcttag
    21121 ccgtattttt gacatttcaa gcagtatgtt ttagcttcct gattttttca ggatttttag
    21181 ataaactttt ttttagctaa cgaatagtga catattaatt tatataacaa aggagaaaag
    21241 gaaaatggaa gaacaaattt tatcaatctt agaagaaata acaggaacag atgaagtaag
    21301 aaaaaatcgt gatattgatc tatttgatga agggttgctt gattctttag catctgttca
    21361 attattagtc gagctagacg gagagctgga tatccaagtc cctgtatctg aattcgagcg
    21421 agaggattgg agcacaccga ataaaatcat tcaacaagca actgcattga agggttgagt
    21481 atgaaaaaga aactttttgc tattttcggc ccggttgttt tagctgttgg cttactgttt
    21541 gtgtttttta cctcctccta tcaagtagat atctctaatc cagaaacgat tagacgtgcc
    21601 tcttcttcca tgtcagtgaa cgtgttgaaa ggagatgcga taaaaaatta tgcattatct
    21661 gaaaaacaat atattccttt ttttggctca agtgaattaa gcagaatcag tccatttcat
    21721 ccgtctgttc tggcagaaaa ataccagcga aactatcgtc catttttgct gggagcaccc
    21781 gggacacaat cgttaagcca atatatgatg atgcgttctg caggagatgc tatgaaaaac
    21841 aagaaagtcg tctttattat ttctcctcag tggttcgtta aaaatggtgt aaaaactgat
    21901 tactttaata cgtattattc tgaacttcaa acatacgact ggttgttttc aatgaagaaa
    21961 gtaacgcctg ctgatcgcta tttagctaga agattattaa ctttctcaaa agtaaaagag
    22021 aatgatacgt tgacggcgat tctgcaaacg atcaaaaaag ggaagctacc tttacctgaa
    22081 tctttgaatc agctgcgcag ccaatggaac atgctgaaga gagaggatga agtattcagc
    22141 aatattgggt tgaaagatcg acagcaaaaa atcgatcatg agtcaaaacg attaccaaaa
    22201 caataccaag aaacagaatt atccatttta gcaaatcaaa ttggagaacg agaaacgacg
    22261 aataatccat tcgggttgaa aaacgatttt tatacgcatc gtatccgagc ccacgagcca
    22321 gagctcaaac agtcacaaaa aa
//
LOCUS       DACEEZ010000042        22026 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20313_2017
            SAMN12639966-rid9879223.denovo.042, whole genome shotgun sequence.
ACCESSION   DACEEZ010000042 DACEEZ010000000
VERSION     DACEEZ010000042.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639966
            Sequence Read Archive: SRR10023158
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 22026)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22026)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741683.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741683.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:44:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,016
            CDSs (total)                      :: 2,962
            Genes (coding)                    :: 2,889
            CDSs (with protein)               :: 2,889
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 36 of 73
            Pseudo Genes (incomplete)         :: 29 of 73
            Pseudo Genes (internal stop)      :: 18 of 73
            Pseudo Genes (multiple problems)  :: 8 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22026
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639966-rid9879223.denovo.042"
                     /isolate="E20313_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(61..1371)
                     /locus_tag="IWO89_001617"
     CDS             complement(61..1371)
                     /locus_tag="IWO89_001617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 28 protein"
                     /protein_id="HAQ0456365.1"
                     /translation="MRTKKGIINALNFKTNDTQQDDTIILQKAIDQGAKEHLPVFIPK
                     GIYLVGALFLKDKSHLIFEEGAVLKGRTDIEAFPEIDTRVAGVEMKWPAAILNVLSAK
                     DILIEGKGIIDGQGDHWWELYWGKDQKSGTRAEYDQKGLRWIADYAIKRPRACLLYHA
                     EHVVVRDLIFQKSGFWNLQITYSNDVLVEKVIIRNNDGPSTDGIDIDSSTNVRVYECD
                     LACGDDCIAIKSGRDGNGARVNRKSSRIEVARCKIRSGYGVTIGSEVSAGVSDVYIHD
                     INFFQSDCGIRMKSSKERGGVIENIRVENLNMIDVQFPFSWIMDWHNEYNRKNSNDLA
                     QMPESWQAVAEEIPESKQMTKVKDIHIKNVQACLSPGYSLPSRAFDLVAFPEKPIEDV
                     CFSHCQITAKEFGRIEAVQNLCFKSCDLSIETGNTAANNTFDNR"
     gene            complement(1383..2801)
                     /locus_tag="IWO89_001618"
     CDS             complement(1383..2801)
                     /locus_tag="IWO89_001618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAQ0456366.1"
                     /translation="MLSQNAVTANTVKKEKKEFGISDKIGYMFGDLGNCFILGLVNSF
                     LMIYYTNVLGVSGAIVGTLFLISRILDAFADVMVGRLCDVSELTETGRFIPWIRKMKY
                     PFCLVTVILFLPFVQHLPMTVKIAYIFVSYIVYGIFLSTINIPYGAMAAAISSNPNDR
                     TSLSTFRSIGSAIGGSTTGFFIPILMYTHNKSGQQVVSGNHFFWISIGCAAIAFVAYM
                     LTCKLTTERVRVVKNEKVSASQLVKGLLANRALIILVIVDIFIVINQILAGTNMTYLF
                     NDYFHNKQAMSVALLFTYGTVVVLAPFATKLTKLFGKKEASVFALLASSCMYLLMYFL
                     HITNAWVYLILLFIATLGSGLFNLMVWAFITDVIDYHQYKTGLREDGTVYGVNSFARK
                     VGQACAGAVGGFMLALIGYQSSTTGGAIQSVLVQERIYTMANLLPAFCLFVSAIILLV
                     GYPLNRSKTVFIGEELKKINER"
     gene            complement(3041..4609)
                     /locus_tag="IWO89_001619"
     CDS             complement(3041..4609)
                     /locus_tag="IWO89_001619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 43 protein"
                     /protein_id="HAQ0456367.1"
                     /translation="MQTIKVKNPILRGFNPDPNILRVKERYYIIVSSFEWLPGIRVYE
                     SKNLVNWSHTTDILTTQVSLQGNPTNGSIWAPQISYADGLFYLVYTDVKSAGRPFKDV
                     HNYLITASEITGPWSEPIYLNSNGFDPSLFHDLETGKKWLVNELWDYRLDTPNKSSGI
                     VLQEYDAEKKKLDGPIYKIFAGTELAKTEAPHLYFRNGYYYLMTAEGGTGRGHSVTVC
                     RAKNITGPYELDPGFPMLTASDKPASTLQCTGHGSLVQTPSGRWYMTYLCTRPLNGAA
                     ILGRETAIQEVYWTSDDWLRLKDSQTTPMEEIMIETNEEVQQEKDHQFMDTFEGELKK
                     NWNARRILPNADWCDTKCRPGFLRLISGESLQSTFEHHLLAIRQTDFTFDAETAFEYM
                     PNNFNQMAGLVLYLNESNYIYCYVTWNEDKGKCLRMIESENGVAQIEDWTIPLTEKKT
                     FLKVTVKKETAQFHLKDVSTEEWKKVGPQKNMCILSGGFTGNFIGIAVHDLNKKRGSY
                     ADFEFFKYDGKDHL"
     gene            complement(4613..4894)
                     /locus_tag="IWO89_001620"
     CDS             complement(4613..4894)
                     /locus_tag="IWO89_001620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4064 domain-containing protein"
                     /protein_id="HAQ0456368.1"
                     /translation="MKRKWELLLGMVGGSLSLIFFGGLAVTLSNMSASEFKKSYQSLA
                     VDHSTLSLENTFGLLQDMTGLFAVVLFISLAFLAAGAFSLYRVRMRKGA"
     gene            complement(4891..5814)
                     /locus_tag="IWO89_001621"
     CDS             complement(4891..5814)
                     /locus_tag="IWO89_001621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pectin esterase"
                     /protein_id="HAQ0456369.1"
                     /translation="MRMKDKLIIGKNAIDTPTDFETIQEGIDYLAGLPDSHPKVLIVK
                     EGRYDEYIESRLSNFKLVGAGTVVISGNRFAKQEYLDGSQLGTFRSATFFLEGSNVVL
                     ENLHIENTAGPGEKVGQAVALFNSGDRVSLKNCRLSGYQDTLCTGPLPDLQKDGTPFC
                     TPASANPEYCRQTYEYCWIEGTVDFIFGGADAVFHQCHIHSLASKNPGYITAASTPKN
                     QKTGYRFESCTLTAEPDTRNVYLGRPWRPYANVLFEACKIGGHVHPTGWDNWDDKKNE
                     QTVKFSERNNNYETSIKRPEWIHLEKQGVQK"
     gene            complement(5811..6689)
                     /locus_tag="IWO89_001622"
     CDS             complement(5811..6689)
                     /locus_tag="IWO89_001622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HAQ0456370.1"
                     /translation="MQLNDFLGAPSSRHTPSGKRLYREKQNGFMLEYYQLYLNELETV
                     PALYAYPEKEGPFPTVLYLHSHGGDFSVGKSELIHGAPYLASPSFAEVLTGMGYAVWS
                     IDAWGFEERGGISESELFKQFLLTGKTLWGMRIYDVISLIDYLETREDTDTQRLATIG
                     LSMGGLLSWWVTALDSRVKVCIDLAAQVDIETLLLHRRLDHHGFYYYVPNLLTAYTTL
                     AIQEKIAPRPRLSLVGKNDTMCLDEGVLKLRKGLDKKYQSMGSPENFSSFSLTGGHME
                     TQEMRNIWKQFIREHL"
     gene            complement(6676..8013)
                     /locus_tag="IWO89_001623"
     CDS             complement(6676..8013)
                     /locus_tag="IWO89_001623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 28 protein"
                     /protein_id="HAQ0456371.1"
                     /translation="MYDILTFGASIDELNTEAIQQAIDAAASDGGGTVVVPAGEFLTG
                     ALFLKSNVELHLSAGAVLKFSDDPKDYPVVHSRWEGVHRKVYASCIYAQNVENISVTG
                     FGTLDGNGKKWWHTFRNEPDNLAYPRPKLMSFHNCHRITVKDIKLIQSPSWTINPILC
                     SNATFDNLTILNPADSPNTDGIDPESCKNVRISNCHIDVGDDCIAIKAGTEDTYERIA
                     CENITITNCTMVHGHGGVVLGSEMSGSIRNITISNCIFQETDRGIRLKSRRGRGGIVE
                     DIRVSNIVMDNVMCPFILNLYYFCGPRGKEPYVWEKKAYPIDERTSAFRRIHFSNITA
                     RNVHASAGFIYGLAEQFIQEITFDNIDVSMAQDAVPGVPAMMTGIEEMSRQGFYIGFA
                     GKVSFNQMSIENHEGPAFLLENTKEISIEKFQSNQSENMDELVVRKNFAYAVE"
     gene            complement(8016..9119)
                     /locus_tag="IWO89_001624"
     CDS             complement(8016..9119)
                     /locus_tag="IWO89_001624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase family 88"
                     /protein_id="HAQ0456372.1"
                     /translation="MENWSKKLAKSIMERTPRLYEEKWYKGKWSYDYGVVLKGFQLLW
                     ERTQEKIYFDFIKDNIDYFVQEDGTIRGYSVEEYNIDHVNTGKLFFLLYKETGEEKYK
                     KAAELLSQQLANHPRTSEGAFWHKEIYPYQIWLDGLYMGSPFYAEFIQNFSEEKDYQD
                     VLRQFEISYKHLFDPKTNLLIHAWDEKKVQPWADPKTGLSHHFWSRSIGWYLMAAADT
                     YELLHEETDCSLLKEILEKTLAALLPYQEENSGTWYQVTTETKRKGNYLEASGSSMFV
                     YAMAKGIRLGLLNKEEWLPQVKKSHQGLLDEFVLETKEGWLNLNKNCQVAGLGGADQR
                     DGSYAYYISEPIICNDQKGVGAFLQALIEVEAL"
     gene            complement(9193..10701)
                     /locus_tag="IWO89_001625"
     CDS             complement(9193..10701)
                     /locus_tag="IWO89_001625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HAQ0456373.1"
                     /translation="MKKATLVKLGLSFAGILALAACGSSSSNSSSETEDGKAKITWLN
                     ILHHASPPTDTIVNLIEEKTDTELTFNWIPDASKEERLTTALASDELGDIVTLQIAML
                     KNSSVRNALKSGVFWDVSEYLKDYKNLSQISEDRIEAASIDGALYGVPIQKDYARAGL
                     VIRKDWLDNLGLDVPTTMDELYEVAKQFTENDPDGNGVDDTVGFGDRAPSDIRYTSFK
                     LFTSYFGAPNGWAVDDNGKFTPEFDTEEYMKALNFSRDLFENGYLAQDFAVTQKTDQQ
                     EQFAQGRTGIYTGMVDIKNLKTMGQGIQDDMELVPVNKISDGEGGGYHVWSEGNGIGG
                     LLAFPKSQVKDEAQLKKLLQFVDDLLEEDVYKLMTGGIEGTHYELNDDGSIEFIDMDL
                     WQQEVQPLSSSRPSEVTFSFKDTDPEKEFSNQLIKENEEFAVMDPSVPLDSPTNNEVG
                     SEIEKIVIDATVQYIMGQLDEDGFKKAVEDWKAQGGDRITQEYEEAYKAAAQ"
     gene            complement(10667..11359)
                     /locus_tag="IWO89_001626"
     CDS             complement(10667..11359)
                     /locus_tag="IWO89_001626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF624 domain-containing protein"
                     /protein_id="HAQ0456374.1"
                     /translation="MLQSLDKINGRLNRILQLVYLNFLWGGLVIAGLGVFTFGPASYA
                     MVAVIRKWLRSKETFPLTKTYFTYFKENYRETLLMSWIYGAIGIVLLVDLSFIHNWYL
                     RIALYIAAFIYVLSALYIFPIMAHYDWKGYFFKMKMAFLFGFSHLHYSLVLILSMVGI
                     YLMIIQTMPGVLTFIGGSLFFSAVTWTANSIFCHLEWKNSYTEKTEENKNIIKTVKEK
                     IYEKSNARKIRS"
     gene            complement(11381..12355)
                     /locus_tag="IWO89_001627"
     CDS             complement(11381..12355)
                     /locus_tag="IWO89_001627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAQ0456375.1"
                     /translation="MESVKKQEPFQLTLSKKEEKLRRRKKIIAGIKTNKFLYVMILPG
                     VLYFLIFRYLPMGGLVIAFQDYQPFLGIMGSPWVGFKHFIRLFTEPTFFTLLRNTLIL
                     FGLNIFVFFPMPIILSLLLNEVTNKRLKNGIQTIIYIPHFMSWVIIVSITYVFLNVDG
                     GVLNEFLATMGFEKISFLTSPEWTRTVYIGQVIWKELGWSTIIYLSAITAVDTQLYEA
                     AEMDGAGRLRKTWHVTLPAIRPVIITLLILKIGQTLDLGFEHMYLLLNSLNRSVAEIF
                     DTYIYTAGLKNGQLSFSTAMGLFKGVVGLFMVILANKIAKKLGEDGVY"
     gene            12655..13527
                     /locus_tag="IWO89_001628"
     CDS             12655..13527
                     /locus_tag="IWO89_001628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HAQ0456376.1"
                     /translation="MKKKVSIGERSFTLFNNTFLILLALICIVPFLNIIATSFASTQE
                     VVAKKFILFPTTFSLDAYRYILSTPTIFRALAVSIGVTGVGTIVSMCATSLMAYGLSR
                     KYLFGRGFVNFLVVFSMLFSGGMIPTFLVVRSLGLVNSYWAMILPVAVNAMNMIIMRN
                     FFQALPDSLEESAKMDGCTDFGVFFKIMLPLALPSIATISLFYAVTYWNTYMTAILYI
                     NDSSKWPIQILLRQIVIVSSGMQAESSAVDVIPPAQTVKMAVIVIATVPMLIVYPFVQ
                     KYFVKGALVGSVKG"
     gene            complement(14109..16307)
                     /locus_tag="IWO89_001629"
     CDS             complement(14109..16307)
                     /locus_tag="IWO89_001629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAQ0456377.1"
                     /translation="MKKSTVFYKIFLPMFLLGAVLVIGFSLFIYQNTYESIESSYLTD
                     KKNLLRQVKTNVEWKIRTIEYSFSTYGSTKNFSDIFKHPLTYTDYSTYSEIRKELNFI
                     ETIVMDDNNYDLVSLAGKWGVINGSLSQLTEEEVEKYQQKYINNKNNLFWQKKNKGIE
                     MVISLPMFEKEKYALGIATIEKHTIEQVVGESGEDILSIENEVGNLFTTQTVKNKEQP
                     TVLTDVDLDYNQLKVIREKDKTYIVLKSDYNNWIYRLEVDSSAIGAMIRNLRIGLVAV
                     SLTLVGLIGLFSYLFSDRFARPISQIQERLNLKSKGFSGKELDLVAQSVSRIIGEKEA
                     LSAHLVTQKPQLETLFVLSLFRNRVERRELDQRLQQFGYSSQNDCYYTALVQIDLLED
                     SHGGERDLLLLAINNMITEIVPKENRMLPIVLNEEMQATIYRMDKDDAEASKKVMEYC
                     RLIQKRVKEYLNLTISIGISNRFETLNESKQSVDRAKEALYYRVNTGPSSIIFYQEIV
                     PAAHEKTLIRYPIEEQNRLFEAIRSGNQEVKNLVHELIDALFAQNKNPLSREVVSVRL
                     INELVQLGQLLGVDSKNFDNMKQIYVKALNNYHPKELEKLVLEQLVLPITECSQAITD
                     HEFKNLSENIMHIVYTEYDRDLSLDVIADRLHYNPNYLSNVFKKETGEKFGDFVQNHR
                     LEVAKKWLKETNLSVKEIAERLQYRNPQNFIRFFKKKESITPGEYRKKYL"
     gene            16539..17531
                     /locus_tag="IWO89_001630"
     CDS             16539..17531
                     /locus_tag="IWO89_001630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family transcriptional regulator"
                     /protein_id="HAQ0456378.1"
                     /translation="MVTIKDIAKAAGVSHTTVSRALNNSSLIKNETKQKIRQLAKEMN
                     YSPNYSAKGLVNQKKYLIGLFFSSLHKGTSSNFLADSIAGIHSVLDTNYSLSVESIDV
                     VDLTEINLQRYDGLIVMSQSDDDQPFIDYLKEKEFPFVVVNRHINDPAVINVTADDAA
                     GVMQAVDYGIGCGHTKIAYIGGKKNFRSTKEREKGVLKSLKKASIPVNLAYFLTGDYS
                     LESGLQNMEYLLSLSDLPTLVFCGNDDIAIGALRAVHLAGLRVPQNISLIGFDDSPVV
                     SYLNPPLTTVRKPLKEMCQEGTKLLLDLIEGRTIEQSKIELSTLLILRESINYL"
     gene            17591..19012
                     /locus_tag="IWO89_001631"
     CDS             17591..19012
                     /locus_tag="IWO89_001631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagaturonate reductase"
                     /protein_id="HAQ0456379.1"
                     /translation="MKKLTKELVKKNEAPVKVLQFGEGNFMRGFIDWQIQQLNNQQLF
                     KGNVAIIQPLEQGLGNMMNQQDNLYTVILQGLSNGEIVDTSEIITVVEQMINPYDEWD
                     AYLSLAENDDLAYIVSNTTEAGIQFVETDNLTDTPPQSFPGKLTAFLYRRFKLNKAGF
                     TIIPCELIDRNGERLKEIILRYAEKWDLEPAFVEWLEKENVFCCSLVDRIVPGYPRED
                     AGVFNQRHNYEDQLMVKAEPFMLWVIEGPKELENQLPFKQAGLNVIITDDMTPYRERK
                     VHLLNGPHTAMVPLALLAELETVEEVMKDPLFSAYVDQLFNLELIPMLSLPKDELAIY
                     ADQIKERFLNPFAHHKLEAISLNSVSKFSTRLLPVFKKYIEEQNQVPPLITVSLAALL
                     LMYRGDQVKPHDDEKTISEFTDAWSDNGTAIPRLLQNAALWGEDLSQIPNVTDTVQEI
                     AAKIDQKGVRSVLQEMKGTMVHA"
     gene            19005..20504
                     /locus_tag="IWO89_001632"
     CDS             19005..20504
                     /locus_tag="IWO89_001632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009224330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="altronate dehydratase"
                     /protein_id="HAQ0456380.1"
                     /translation="MLETLQPTMKLNRRDSVVVALTPIPKQTQLTIDQQTITTLEDIP
                     QGHKIALGDLKAGDNVIKYGYPIGHVTTDVTAGQWLHTHNVKTNLSGELDYRYEKDVH
                     PSHYLFENRTFQGYLRKNGKVGIRNDLFLVPTVGCVNGIAELIVKQFKEKHPDLGQFD
                     HITILKHPYGCSQLGKDHQNTREILADAVNHPNAGGVLVFGLGCENNTVPEFKKILGE
                     YDEERVKFLVAQDVYDEIEQGVALLEELLTAAENDQRISVPLSKLNVGLKCGGSDGLS
                     GITANPLLGAFSDYLIAQGGSTILTEVPEMFGAEQVLMARAENEEVFDSIVDLINDFK
                     QYFLSYGEPVYENPSPGNKAGGITTLEDKSLGCTQKSGSSPVVDVLQYGEKIRKPGLS
                     LLQAPGNDLVAASALASSDCQLVLFTTGRGTPFGSYVPTVKVSTNTTLFERKGHWMDF
                     NAGVLLEQPMEVILESFVQKIIAVASGEETKNEQNDVREIAIFKNGVTL"
     gene            20543..21937
                     /gene="uxaC"
                     /locus_tag="IWO89_001633"
     CDS             20543..21937
                     /gene="uxaC"
                     /locus_tag="IWO89_001633"
                     /EC_number="5.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronate isomerase"
                     /protein_id="HAQ0456381.1"
                     /translation="MFLSDDFLLKDDWAKKLYHSYAKNMPIIDYHCHLDPKEIYENNN
                     FSNLTEAWLGGDHYKWRLMRACGVPEEKITGNASDFEKFLAWCQTVPKIIGNPLYSWS
                     HLELKRFFGIDLLINEENAEKIWTQANEILAAPDFRRREIAKKSNVEVICTTDDPADD
                     LHYHQLLAHEEPDLKVLPSFRPDKALNIEKEGFNAWVTLLEEAADQTITTYQELIEVL
                     GQRIEYFHQHGCRVSDHALDILRYKEADETVLEEIFQKARANKLLSSDEIDAYLTETL
                     IHLTHFYHSHNWTMQLHIHAYRNCNTQMLEKLGPDTGYDGMNDLSLTIPLQKLLNRAE
                     ETDQLPKTILYSLNPNDYPAILALMGCFQKEQNGKLQLGSGWWYNDTRAGMRDQLTQF
                     ADGSALGNFVGMLTDSRSFLSYTRHEYFRRVLCEFLGEMVMRGEAPEDEQLLGSLVQA
                     ISYTNAKNYFGFFA"
BASE COUNT         6669 a         4494 c         3823 g         7040 t
ORIGIN      
        1 atcattaaaa aaaataaagg tcaagctaaa atattattta agcttgacct tattataatt
       61 tcaacgattg tcaaaagtat tgtttgcagc tgtgttgcca gtttctatag ataaatcaca
      121 gcttttgaaa cataagtttt gtactgcttc gattcgcccg aattcttttg cagttatttg
      181 gcaatgagaa aagcagacat cttctatagg cttttcagga aatgctacta aatcaaaagc
      241 tcgggaagga agtgagtaac ctggagaaag gcatgcctgt acgtttttga tgtgtatgtc
      301 tttgactttc gtcatttgct ttgattcagg tatttcttca gccactgctt gccaactttc
      361 tggcatttga gcaaggtcat tgctattttt tctattgtat tcattatgcc agtccattat
      421 ccaagaaaaa ggaaattgta catcaatcat attcagattt tctaccctga tattttcgat
      481 cactccccct ctttcttttg aagatttcat tcgaatacca cagtctgact gaaaaaaatt
      541 gatatcgtga atatatacgt cactaactcc agcactaact tcgctaccaa tagttacacc
      601 gtatcctgaa cggattttac atctagcaac ctcgattctt gatgattttc gattgactct
      661 tgcaccattg ccatctctgc cagacttaat agcaatacaa tcatcaccac aggccaaatc
      721 acattcatag acacgcacat ttgttgaaga atcaatatca atcccatctg tacttggtcc
      781 atcattgttg cggatgatta ctttttctac taatacatcg ttagaataag tgatttgcaa
      841 gttccaaaaa cctgatttct gaaaaatcaa atcacgaaca acaacatgtt ctgcatgata
      901 cagtaaacaa gcgcgtggac gtttaatagc ataatccgca atccaacgaa gtcccttttg
      961 atcgtattca gctcttgtcc cgcttttttg atcttttccc caatacagct cccaccaatg
     1021 gtctccttga ccgtcgatta ttccttttcc ttctataaga atatctttcg ccgagagaac
     1081 attcaaaatc gctgctggcc atttcatttc tacgccggct actcgtgtat cgatttctgg
     1141 aaatgcttcg atatctgttc tccccttgag gactgcacct tcttcaaaga taagatgact
     1201 cttatctttt aaaaaaaggg ctccgactaa ataaatgcct tttggaataa atacaggtaa
     1261 gtgttctttc gcaccttgat cgattgcttt ttgcagtatt attgtatcat cttgttgagt
     1321 gtcgtttgtt ttaaagttca aagcattgat aatccctttt tttgtccgca atacgtttct
     1381 ccctatcttt cattgatttt tttcaattct tcaccaataa atacagtttt tgaacgattc
     1441 agtgggtaac ctactagcaa aatgattgcg gatacaaata agcagaatgc tggtaacaag
     1501 tttgccatcg tataaatcct ttcttgtact aaaactgatt ggatggctcc acctgttgta
     1561 gacgattggt aaccaatgag tgccagcata aatccgccta ccgctcctgc acatgcttgt
     1621 cctacttttc tcgcaaaaga atttactcca tatactgttc catcttctct taagcctgtc
     1681 ttatattgat gataatcaat cacgtcagta atgaatgccc agaccatcaa attgaataaa
     1741 ccagatccta aagtagcgat aaataaaaga atcagatata cccatgcatt tgttatatga
     1801 aggaaataca tcagcaagta catacatgaa gaagcaagta acgcaaatac gcttgcttcc
     1861 tttttaccaa ataattttgt cagctttgta gcaaatggtg ctaaaactac tactgttccg
     1921 taagtaaata aaagagcaac agacattgct tgtttattat ggaagtaatc attgaataaa
     1981 taagtcatat tcgtcccagc taaaatctga ttgatcacaa tgaaaatgtc aacaatgact
     2041 aaaataatca gtgctcgatt agctaacaaa ccttttacca actgagaagc agatactttt
     2101 tcgtttttga caactcttac tctttcagta gtcaatttac aagttaacat atatgcgaca
     2161 aatgctattg cagcacatcc gattgaaatc caaaagaaat gattccctga aacgacttgt
     2221 tgtccactct tgttatgggt atacattaaa atcggaatga agaatcctgt agttgatccg
     2281 ccaatcgcag aaccaatgct tcggaacgtg gacaacgagg tgcggtcatt aggatttgag
     2341 ctaattgctg ctgccattgc tccatacggg atattgattg ttgagagaaa aatcccataa
     2401 acaatgtatg aaacaaaaat ataagcaatc ttcaccgtca ttggaagatg ttggacgaat
     2461 ggcaaaaaca aaatgacagt cactaagcaa aatggatact tcatttttct gatccaagga
     2521 ataaatcggc cagtttctgt taactctgag acatcacata atcttccaac catcacatca
     2581 gcaaacgcat ccaaaattct cgaaatcaag aataacgtac ctacgattgc ccctgaaacg
     2641 ccaagaacat tcgtataata aatcatcaag aaactattga ctaaacctaa aataaaacag
     2701 ttacctagat caccgaacat gtagcctatc ttatcggaaa tcccaaattc ctttttttct
     2761 ttttttactg tatttgctgt aacagcattt tgtgaaagca tttgaattcc tcctacctct
     2821 tctagtaaac gttttcaaaa ttataactct ttttttcttg tttttatatt cacaatgttg
     2881 gctttttatt gcattatctt gacagataaa aaacaaaaaa taatctaaaa acattgatgc
     2941 ataagattga taactttttt tacataacaa tatatacaaa caaaaaaatt cactaactag
     3001 tgaaaataag ttagtgaacc tcatttatta aagaattaaa ttacagatga tcttttccat
     3061 catacttgaa aaactcaaaa tcagcataac ttcctctttt tttatttaga tcatgcactg
     3121 ctatcccgat aaaatttcca gtaaaccctc ctgagagtat acacatgttt ttttgcggac
     3181 ctactttctt ccattcttca gttgatacgt ctttcaaatg aaactgtgct gtctctttct
     3241 ttacagtgac tttcaaaaaa gtcttctttt ctgtgagtgg aatcgtccaa tcttctattt
     3301 gtgctactcc gttttctgat tctatcatac gtaggcattt tcccttgtcc tcattccaag
     3361 taacataaca ataaatataa ttggattcat ttaagtacag taccagtcca gccatctgat
     3421 taaaattatt aggcatgtat tcaaatgctg tctccgcatc aaaagtaaaa tccgtttgcc
     3481 gaatagctaa caaatgatgt tcgaaggtag attgaagaga ttcaccggaa atcagacgta
     3541 aaaaacctgg tctacatttt gtatcacacc aatcagcatt tggcaaaatg cgtctggcat
     3601 tccagttctt ctttagctct ccttcaaatg tatccataaa ctgatgatct ttttcttgtt
     3661 gtacctcttc atttgtctca atcattattt cttccatcgg agttgtctgg ctatctttaa
     3721 gtcttaacca atcgtcggac gtccaataca cttcttgaat agcggtttct cttcccaaaa
     3781 tagccgcacc gtttaatggt ctggtacaaa gatatgtcat gtaccatctt cctgacgggg
     3841 tttgtactaa gctcccatgt cctgtacatt gcaatgtaga agcaggtttg tcactagctg
     3901 tcagcatagg aaaaccagga tcaagctcgt aaggaccagt aatattcttt gctcggcaaa
     3961 ctgtaactga atgtcctctt cctgtaccgc cttctgccgt catcaaataa taatacccgt
     4021 tacgaaaata caaatgtggt gcctccgttt tcgctaattc tgtacctgca aatattttat
     4081 agatgggacc atcaagcttc ttcttctctg catcatattc ttgtaatacg ataccgctgg
     4141 atttattcgg tgtgtccaga cgataatccc atagttcatt gacaagccat ttttttcccg
     4201 tttcaagatc atggaacaaa gaaggatcaa agccgttact atttaaataa attggttcgg
     4261 accaaggacc tgtgatctca gatgcagtga tcaaataatt gtggacatct ttaaacggac
     4321 ggcctgcact tttgacatct gtgtaaacaa gataaaacaa tccatcagca taactgatct
     4381 gtggcgccca tatacttcca tttgtaggat ttccttgcaa agatacttga gtggtcaaaa
     4441 tatccgttgt atgtgaccag ttgactaaat ttttcgactc atatactcgg attcctggaa
     4501 gccattcaaa agaggagaca ataatgtaat accgctcttt cactcttaat atattaggat
     4561 ctggattgaa tcctcttaaa ataggatttt tcactttgat agtctgcatg attcaggctc
     4621 cttttctcat tcttactcga tataaactga atgccccagc tgctaaaaaa gcaagtgaaa
     4681 taaacaacac aacggcaaac agaccagtca tgtcctgtaa taatccaaat gtattttcta
     4741 aagacaaagt tgaatgatct acagcaagcg actgataact ttttttaaac tctgacgcag
     4801 acatattact taaagtaact gctaacccgc caaagaaaat caaagataga ctgccaccga
     4861 ccattcctag caatagttcc cattttcttt tcatttctga accccctgct tttctaaatg
     4921 tatccattca ggtcttttga tcgatgtttc ataattgtta ttacgttcag agaatttgac
     4981 agtctgttca tttttcttgt catcccaatt atcccaacca gttgggtgaa catgcccacc
     5041 tatcttacac gcttcaaata agacatttgc atacggacgc cacggtcttc ctaaatagac
     5101 gtttctagta tcaggttctg cagtcaaggt acagctttca aaacgatacc ctgttttttg
     5161 atttttcggc gtagatgctg ctgtgatgta tccaggattt ttactggcta agctatggat
     5221 atgacactga tgaaaaacgg catctgcccc accaaatatg aaatccaccg ttccttcaat
     5281 ccagcaatat tcataggtct gacgacaata ctcaggattg gcagaagccg gtgtacaaaa
     5341 aggagtacca tctttttgca aatcaggcaa cggacccgtg cataaggtat cctgataacc
     5401 agatagtcga cagtttttta aagatacgcg atctcctgaa ttgaataaag caacagcctg
     5461 tcctaccttt tctcctggac ctgctgtatt ttcgatgtga agattttcta atacgacatt
     5521 cgatccttct aagaaaaaag tagcacttcg gaaagtccct aactggctac cgtcaagata
     5581 ctcctgtttt gcaaaacgat taccggagat aactactgtt cctgctccca caagtttaaa
     5641 attggataat ctgctttcta tatattcatc atatcttcct tcttttacta tcaagacctt
     5701 tggatggcta tccggcaggc ctgccaaata atcgattcct tcttggatag tttcaaaatc
     5761 cgtcggggta tctatagcat tctttccaat tatcagtttg tctttcatcc tcataaatgc
     5821 tcccttatga attgtttcca aatatttctc atttcttgtg tttccatatg accgcctgtt
     5881 aaagaaaaag aagaaaagtt ttctggacta cccattgatt gatacttttt gtccagccct
     5941 tttctcagct tcagtacgcc ttcgtccaag cacatcgtat cgttttttcc aactaagcta
     6001 agacgagggc gcggagcgat tttttcttgg attgctaacg ttgtataagc tgtcaataga
     6061 tttggcacat agtaataaaa cccatgatga tctaggcggc gatgtagcaa aagtgtttcg
     6121 atgtccacct gtgccgctag atcaatacaa actttgacac gagaatccaa tgctgtcacc
     6181 caccagctta ataagcctcc catagaaaga ccaatcgttg cgagccgttg agtatctgtg
     6241 tcttcccttg tctccaaata atcgatcaag ctgatcacat cataaatacg cattccccat
     6301 aacgtttttc ctgttaataa aaattgtttg aacaactcgc tttcagaaat acctccgcgt
     6361 tcttcaaatc cccaagcatc aatcgaccaa acagcatatc ccattcctgt cagtacttct
     6421 gcaaaagatg gagaagctaa gtaaggagca ccatggatca attcactttt cccgactgaa
     6481 aaatctccgc cgtgagaatg caggtaaagg accgtcggaa acggtccttc tttttctgga
     6541 taagcataga gggctggtac tgtttccaat tcatttaagt acaattgata gtattcaagc
     6601 ataaaaccgt tttgtttttc tcggtataat cgttttccac taggggtatg tctgctagat
     6661 ggagcaccta aaaaatcatt caactgcata agcaaagttt ttacgaacaa ccagttcatc
     6721 catattttct gattgatttg attggaattt ctcaatactg atttctttag tattttctaa
     6781 tagaaatgct ggtccctcgt gattttcaat cgacatctgg ttaaaggaaa ccttacccgc
     6841 aaagccaata taaaatcctt gtctgctcat ttcctctatt cctgtcatca tagcaggaac
     6901 accgggaact gcatcttggg ccatcgagac atcgatattg tcaaatgtga tttcttgtat
     6961 gaattgttcc gctaatccat aaataaaacc tgcagaagca tggacattcc ttgccgtgat
     7021 attcgaaaaa tggatccttc tgaatgctga tgtccgttca tctatcggat aagccttttt
     7081 ttcccataca tacggttctt tccctcgcgg accacagaaa taataaagat tcagtataaa
     7141 tggacacatc acattatcca tgactatatt ggatactcga atatcttcaa cgatcccgcc
     7201 tcgacctcgt ctagatttca atcgtattcc tcgatcagtt tcttggaaga tacaatttga
     7261 aatggtaata ttacgaatac taccactcat ttcacttcct aatacgacac cgccatgtcc
     7321 atggaccatc gtgcaatttg tgatcgtgat attttcgcaa gcgatccttt cgtacgtatc
     7381 ttcagtccct gccttgattg caatacagtc atctcccacg tcaatatggc aattactaat
     7441 acgcacattt ttgcatgatt ctggatcaat tccgtctgta tttggtgaat ccgctgggtt
     7501 caagatcgtc aagttatcaa acgttgcatt actgcaaaga atcgggttga tcgtccaact
     7561 tggtgactgg atcaatttaa tatctttcac agtaattcga tgacagttat ggaaactcat
     7621 caatttagga cgaggataag cgagattatc tggttcattt ctaaaagtat gccaccattt
     7681 ttttccattt ccatctaatg taccgaaacc agtgacagag atgttctcta cattttgagc
     7741 atagatacag gaagcataaa cttttcgatg gactccttcc caacgtgaat gaacaacagg
     7801 ataatctttt ggatcgtcag agaatttcag aaccgctcct gcgcttaaat gaagttcgac
     7861 attgcttttt aaaaataaag ctcctgttag aaattcacct gctggaacaa ctacggtccc
     7921 gcctccgtca cttgctgcag catcaatggc ttgttgtatt gcttcagtat tcaattcatc
     7981 aattgatgcg ccaaatgtca aaatatcata catatttata atgcctccac ttcaatcaat
     8041 gcttgcaaga atgcaccaac gcctttttga tcattacaaa taattggttc actgatgtag
     8101 taagcatatg aaccatctcg ctgatctgct ccgcctaacc ctgctacttg acagttttta
     8161 ttcaagttca gccagccttc ttttgtttcc agaacaaatt catcaagtaa tccttgatgt
     8221 gatttcttca cttgtggaag ccattcttct ttgttcagca gtcccaaacg tattccttta
     8281 gccattgcgt aaacaaacat actactgccg gaagcttcca gataatttcc tttccgtttt
     8341 gtttctgttg ttacttgata ccatgtcccg ctgttttctt cttgatatgg aagtaatgca
     8401 gccagtgtct tttctagaat ttctttcagc aaagaacaat ctgtttcttc atgaagtaat
     8461 tcataggtat ctgctgcagc catcaaatac cagccaatcg atcggctcca aaaatgatgg
     8521 gatagacctg tttttggatc agcccatggt tggaccttct tttcatccca tgcatgaatc
     8581 agcaaatttg tttttggatc gaatagatgt ttataactga tttcaaactg tctcaaaaca
     8641 tcttggtaat ctttttcttc actaaaattc tggataaatt cagcataaaa aggagaaccc
     8701 atatataagc catccaacca aatttgataa ggataaattt ctttatgcca aaaagctcct
     8761 tcggatgtgc gtggatggtt tgccaactgc tgggaaagga gttctgctgc ttttttatat
     8821 ttttcttcgc ctgtttcttt gtacaataaa aagaaaagct ttcctgtatt tacatggtcg
     8881 atattgtatt cttcaacgct gtagccacgg atcgttccat cttcttgcac gaaatagtcg
     8941 atattgtctt ttatgaaatc aaaataaatt ttttcttggg ttcgttccca aagtaattgg
     9001 aaccctttaa gtacgacgcc atagtcataa gaccattttc ctttatacca cttttcttca
     9061 tataagcgag gtgttctttc catgatactt tttgctaatt tctttgacca gttttccatt
     9121 atttgctctc ctttaaaaat taaaatagga tttgggacaa ataccaagaa gtatttgtcc
     9181 caatcgtcta tattactgtg ccgctgcttt gtatgcttcc tcgtactctt gtgtgattcg
     9241 atcgcctcct tgtgctttcc agtcttctac agcttttttg aatccatctt cgtcaagctg
     9301 tcccatgatg tattgcaccg tagcatcaat gacgattttt tcaatttctg atcctacttc
     9361 attgttcgtc ggtgaatcca atggcacaga aggatccatt acagcaaatt cttcattttc
     9421 tttgatcaat tgattagaga attctttttc agggtctgta tctttgaaag agaacgtcac
     9481 ttcactagga cgagagcttg ataacggttg cacctcttgt tgccaaagat ccatatcgat
     9541 aaattcgata gaaccatcat cattcaattc ataatgcgtt ccttcgatac cgccagtcat
     9601 caatttatac acatcttctt ctaaaagatc atcaacaaat tgaagtaatt tcttcaattg
     9661 tgcttcgtct tttacttgag atttgggaaa tgctagcaag ccgccaatac catttccttc
     9721 tgaccaaaca tgataaccgc cgccttcacc atctgagatc ttgtttacag gcactaattc
     9781 catatcatct tgaatacctt gacccatcgt cttcagattc ttgatatcca ccattccagt
     9841 gtagattcca gttcttcctt gtgcaaattg ttcttgctga tcagtctttt gggtaactgc
     9901 aaagtcttgt gccaaatagc cattctcaaa tagatctctt gaaaaattca aagctttcat
     9961 atactcttct gtatcaaatt ctggagtgaa tttgccatta tcgtctacag cccagccatt
    10021 tggtgctcca aaataagaag tgaaaagttt aaagctggta taacgaatat cactaggcgc
    10081 acgatcgcca aaaccaactg tgtcgtccac accatttcca tcaggatcgt tttctgtaaa
    10141 ttgtttagct acttcataaa gctcatccat ggtagttgga acatctagtc ctagattatc
    10201 caaccagtct tttcgaatga ctaagccagc acgagcgtag tctttttgta tcggtacgcc
    10261 gtacaaggca ccgtcgatac tcgcagcttc gattctatct tcagaaattt gacttaaatt
    10321 tttatagtct tttaaatatt ctgatacatc ccagaatacg cctgatttta acgcattgcg
    10381 gacagaagag tttttcaaca tcgcaatttg aagtgtaacg atatctccta gttcatcaga
    10441 agccaatgca gttgttaatc tttcttcttt tgaagcatca ggaatccagt tgaatgtcag
    10501 ttccgtatcg gttttttctt cgatcaagtt cacaatcgtg tctgtaggcg gtgaagcatg
    10561 atgcaaaata tttaaccaag tgattttagc ttttccatct tctgtctctg aagaactgtt
    10621 acttgaactg ctgccgcatg cggcaagtgc tagaattcct gcaaaactaa gacctaattt
    10681 tacgagcgtt gcttttttca taaattttct ccttcactgt tttaataata tttttgtttt
    10741 cttctgtttt ttctgtatat gagttcttcc attccaaatg gcaaaatata gaatttgctg
    10801 tccacgtgac tgcggaaaag aagagactgc caccgataaa ggttaaaaca cctggcattg
    10861 tttggatgat catcagataa atccctacca tacttaatat caacactaat gagtaatgaa
    10921 gatgtgagaa tccgaataaa aatgccattt tcattttgaa aaaatatcct ttccaatcat
    10981 aatgagccat gattgggaaa atatagagtg ctgagagcac atatatgaac gcagcaatat
    11041 acaaagcgat cctaagatac caattgtgta tgaaagaaag atccacaagt aaaacaatcc
    11101 cgatggcgcc atatatccag ctcatcagca aggtttcacg atagttttct ttaaaatatg
    11161 tgaagtacgt tttcgtcaaa ggaaacgttt cttttgagcg cagccatttt ctaatcactg
    11221 caaccatcgc atacgatgca ggaccgaaag taaaaactcc caaacctgcg atgactaaac
    11281 cgccccacag aaaattcaaa taaaccaact gtaaaatacg atttagcctt ccgtttattt
    11341 tatctaatga ctgcagcata tctttccctc tctttctatc ttagtaaacg ccgtcttctc
    11401 ctagcttttt ggcaatctta ttagctaaaa ttaccataaa caatcctacg acccctttaa
    11461 acaatcccat tgctgtactg aaacttaact gtccattctt caatccagct gtataaatat
    11521 aggtgtcgaa aatttctgca acgcttcgat tcaatgaatt caataacaag tacatatgct
    11581 cgaaccctaa gtctaatgtc tgaccgattt ttagaatcag caaggtaatg attactggac
    11641 gaattgccgg caatgtaaca tgccatgttt tgcgtaagcg tcctgctcca tccatttctg
    11701 cagcttcata caactgtgta tcgactgcag ttatcgctga aaggtagatg atcgtagacc
    11761 agcccaactc tttccagatc acttgtccga tatatactgt gcgtgtccat tcaggggatg
    11821 ttagaaaact gattttttca aatcccattg ttgcaaggaa ttcattcaat acccctccgt
    11881 caacattcaa aaatacataa gtaatcgaaa caatgattac ccaagacatg aagtgaggga
    11941 tgtaaatgat cgtttgaatc ccgtttttca atcgtttgtt cgttacttca tttaatagta
    12001 aagacaaaat gattggcatc ggaaaaaaca cgaagatatt taatccgaac aagatcaacg
    12061 tatttcttaa taatgtaaag aatgtcggtt ctgtaaacaa acgaatgaaa tgtttgaaac
    12121 cgacccaagg acttcccatt attcctaaaa atggttgata atcttgaaaa gcaataacga
    12181 gtccccccat aggaagatac ctaaaaatca agaaataaag gactcccggc agaatcatca
    12241 catataaaaa tttattcgtt ttgattcctg caattatttt ttttcgtctt ctgagctttt
    12301 cttctttttt cgatagcgtc agttgaaatg gttcttgttt ctttacactt tccataagcc
    12361 cctccttagt tttgtgaatg tttgctgctt atgacttcat gatagataac tatctgaatt
    12421 acgtatataa tcatttttat cgtaagtaaa aacgcttaca tagcaatgat ttttgtgcta
    12481 tacaaaacga taaagtgtat tttttcacaa ataaactacg catagcaaac cttctatttt
    12541 tttctatctg acacttagca taggaaatga ttatatacgt aatctttcac cgatgataaa
    12601 ttgaacatat agaaacagaa acatttaatt tattttgagg aggagcctat ctatatgaaa
    12661 aagaaagtct ctataggaga aagaagtttt acacttttca acaatacgtt tctaattttg
    12721 ctagctttga tctgtatcgt tcctttttta aatatcatcg ctacatcttt tgcttctaca
    12781 caagaagtcg tcgcgaaaaa gtttatcctt tttcctacta ccttttcttt agacgcgtat
    12841 cgctacattc tttctacgcc gaccattttt agagcacttg ctgtttcgat tggtgttaca
    12901 ggtgtcggaa cgatcgtcag tatgtgtgca acttcactga tggcctacgg tctttccaga
    12961 aagtatttgt ttggtcgagg tttcgtcaac tttcttgttg tattttctat gttgttcagc
    13021 ggggggatga ttccaacgtt cttagttgtt cgttctctag ggctggtcaa ttcttactgg
    13081 gccatgatcc tgcctgtggc tgttaatgca atgaatatga tcattatgag aaactttttc
    13141 caagccttgc cggatagttt ggaagaatca gcaaaaatgg atggttgcac agattttggt
    13201 gtatttttca aaattatgct gccattagct ttaccgtcta ttgctactat ctcgcttttt
    13261 tatgctgtta cttactggaa cacttatatg actgctattt tgtatatcaa cgattcatct
    13321 aaatggccga tacagattct tttacgccaa atcgtcattg tatcaagtgg gatgcaagca
    13381 gaaagtagtg ccgttgatgt gattccacca gcacaaacag ttaaaatggc tgttatcgtt
    13441 attgcaacag tgcctatgct gattgtttat ccttttgttc agaaatattt tgtcaaagga
    13501 gcactcgtag gttctgtcaa agggtgatta tgtactgaac gcaaaatggt agttgtgtca
    13561 gaagtggtca gcttcaagaa ataagaaggc atttacgaaa attgcttctt aaatttttgt
    13621 gaaatttcgg cttatttttc gaagaatcta ctcctggaac accgtgtatc agaggttgtg
    13681 aaagagacgt tcagccttga gcgatgataa aaactctcac aaacattgcg acgagtaacc
    13741 cacaggagca ataagaagcg attttgaaaa atagcttccc atatttcaca aaattatgcc
    13801 ttattgtcga tagcctgtct cttgaacgcc atttattcgt aaaaaagaac tgtgacaaga
    13861 cttttgtcac agttcttttt gcgtgcttag ccttgaaatt taactatcca taaatacaaa
    13921 tactagggtt attctaactt cttttattgc ttgaatcagt ccacaagaaa aacagcctct
    13981 tgattaaacg gcgtttatct tgttgattct gcggcaataa aatcagccag cagcaaaaca
    14041 tgaagtgctg ttttttgctg tcctctttta ttgcttgaat cagatcacaa gaaaaacagc
    14101 ctcttgattc acaaatattt ttttcgatat tctcccggcg taatcgattc cttcttttta
    14161 aagaaacgaa taaaattttg cggatttcga tattgcaatc tttctgcaat ctcttttacg
    14221 gagaggttag tctctttcaa ccattttttt gctacctcta aacgatgatt ttgaacaaaa
    14281 tcaccaaatt tttctcctgt ttccttttta aatacattac ttaaatagtt agggttataa
    14341 tgcaacctat cagcgatcac atctaaagac agatcacgat catattctgt gtagacgata
    14401 tgcataatat tctccgacaa atttttaaat tcatgatcag tgattgcttg agagcattct
    14461 gtgattggca gaactaattg ttctaaaaca agtttttcta gttctttggg atgataattg
    14521 ttcagtgctt ttacataaat ttgtttcatg ttatcaaagt tttttgaatc tactcccaat
    14581 aactgtccta attgaacgag ttcattgatc aagcggaccg aaactacttc ccgactcaaa
    14641 ggatttttat tttgtgcaaa caatgcatca atcaattcat gtactaaatt cttcacttcc
    14701 tgatttccag aacgaatggc ttcaaacaat cgattttgtt cttctatcgg atatctaata
    14761 agtgtttttt catgagcagc aggaacgatt tcctgataaa aaataattga acttggacca
    14821 gtatttacac gataatacag ggcttctttt gcccgatcaa ccgattgctt gctttcattc
    14881 agcgtttcga accggttact gatgcctata ctgatagtta aattcaagta ttcttttaca
    14941 cgtttttgaa tgagtcggca atactccatg acttttttgc ttgcttctgc atcatcttta
    15001 tccattcgat aaatagtggc ttgcatttct tcattcaaaa cgatcggcag cattcgattt
    15061 tcctttggca caatttccgt gatcatgttg ttgatcgcta aaagtagcaa atcacgctcc
    15121 ccaccgtgac tatcttctaa cagatcgatt tgaaccaaag ctgtataata gcagtcattt
    15181 tgagaactat aaccaaattg ctgcaaccgc tgatctagtt ctcgcctttc tactcgattt
    15241 ctaaataaac taagtacaaa tagcgtttcc agctgtggct tttgggtaac taagtgtgca
    15301 cttaacgctt ctttttctcc tatgatccga ctgactgatt gagcgaccaa atctagttct
    15361 ttcccactga atccttttga tttaagattc agacgttcct gtatttgaga aatcggacgg
    15421 gcaaatctgt ccgaaaataa gtaggaaaaa agaccaatga gtcctaccag tgttaaagac
    15481 actgcaacta gcccgattct cagattgcgg atcattgcgc caattgcact agagtcaact
    15541 tctaatctgt aaatccaatt attataatct gatttcagta ctatatacgt tttatctttt
    15601 tctcttatca ctttcagctg attgtaatct agatccacgt ccgttaatac agttggttgc
    15661 tctttatttt ttactgtctg agtagtaaaa aggttgccaa cttcgttttc aatagaaagt
    15721 atgtcttctc cactttcacc aacaacttgt tctattgtat gtttctctat cgtagcaatt
    15781 cccaatgcat atttttcttt ttcaaacatt ggcaaagaaa tcaccatttc gattcctttg
    15841 tttttctttt gccaaaataa attatttttg ttatttatgt acttctgttg atacttttca
    15901 acttcctctt cggtcagttg actcaacgat ccattgatta ctccccattt accagccaaa
    15961 ctgactaaat cataattatt atcatccata acaatcgttt ctataaaatt tagttctttc
    16021 ctgatttcag aataagtgga gtaatctgta tatgtcagcg gatgcttgaa tatgtccgag
    16081 aaatttttgg tcgaaccata cgtagaaaac gaatattcaa tcgttctaat tttccattca
    16141 acgttagttt taacttgcct gagcaaattc tttttgtctg ttaaataact cgattcgatc
    16201 gattcatagg tattctggta aatgaataaa ctaaatccta tgacaagtac ggctcctaat
    16261 aaaaacatcg gtaaaaatat tttataaaat actgtacttt tcttcatttt cttcctctcc
    16321 tccttttaaa ataaaaggct ttcatttttc tgtaaaattg atactcatct aattaaatga
    16381 gcattccccg ctttcaagaa tagcataatt aaaaataaaa atccaagtaa tttttaacgt
    16441 gtgcaatctt ttcatatcaa agatctattg acttatcgta gttatcgtaa tatgatgaat
    16501 gcgttaacaa aaagtgaaaa agtaaggaaa atgatggaat ggtaacgatc aaagatatcg
    16561 ctaaagcagc tggcgtttct catacgacag tttctcgtgc tttgaataac agcagtttaa
    16621 taaaaaatga aacaaaacaa aaaatacgac aactagctaa ggaaatgaat tattcgccaa
    16681 actatagtgc aaaaggttta gtgaatcaaa aaaaatatct aatcggacta tttttctcaa
    16741 gtttgcataa aggaacttca tcaaatttcc tagcagattc cattgcaggc atccattcag
    16801 tattagacac aaattacagt ttatctgttg aaagtatcga tgttgttgat ttaacagaaa
    16861 tcaacctgca acgctacgat ggactgatcg ttatgagtca aagtgatgat gatcagcctt
    16921 tcatcgacta tctcaaagaa aaagaatttc ctttcgttgt tgtcaatcgt cacatcaatg
    16981 atccagcggt tatcaacgtc actgccgatg atgcggccgg tgttatgcag gcagtagatt
    17041 acggtattgg atgcggacat acgaagatcg cttatattgg cggaaagaaa aattttcgtt
    17101 cgacaaaaga acgggaaaaa ggggtattga aaagtctaaa aaaagcgagc atccctgtca
    17161 atctagccta ttttttaaca ggagattaca gcttagaaag cggcctccaa aacatggaat
    17221 acctattatc actttctgac ctgcctactc tggttttttg cggcaacgac gacatagcga
    17281 ttggtgcatt gagagctgta catttagctg gcctccgtgt tccccagaac atttctctga
    17341 tcgggttcga tgattctcca gtcgtctcct atctcaatcc gccgcttacc accgtacgta
    17401 agcctttgaa agaaatgtgt caagaaggaa caaagctttt actagatttg atcgaaggca
    17461 ggacgatcga acaatctaaa attgagctct ccaccttgtt gatccttcgt gagtcgatca
    17521 attacttgta aactacaagt aattttatat aaaaatgtta acgtgtgcat aacacagaaa
    17581 ggaagtttat atgaaaaaac tgacaaaaga attagtgaaa aaaaacgaag cacctgtaaa
    17641 agttcttcag tttggtgaag gcaattttat gcgtggattt attgattggc agattcagca
    17701 gctgaataac caacaattgt ttaaaggtaa tgttgcaatc attcaacctt tagaacaagg
    17761 tcttggaaat atgatgaacc agcaagataa tttatatacg gtcattttac aaggactcag
    17821 caatggcgaa atcgttgata cttccgagat tatcaccgta gtagaacaaa tgatcaatcc
    17881 ttatgatgaa tgggatgctt atctatctct agcagaaaat gacgacttgg cctatatcgt
    17941 ctccaataca acagaggcag gaatccaatt cgtggagact gataatttaa cagatactcc
    18001 cccacagagc ttcccgggca aactgacagc ctttttatac cgacgtttca aattaaacaa
    18061 agctggcttt accatcattc cttgcgaact gattgacaga aacggcgaac gtctaaaaga
    18121 aatcatttta cgctacgcag aaaaatggga tttagaacct gcttttgttg agtggttaga
    18181 aaaagaaaat gttttctgtt gtagtttagt tgatcgcatc gtcccaggtt atcctcgcga
    18241 agatgcaggt gttttcaatc aacggcataa ctatgaagat caattgatgg tcaaagcaga
    18301 accattcatg ctttgggtga tcgaagggcc taaagaattg gagaatcaac taccattcaa
    18361 acaggctggc ttgaatgtca tcatcacaga tgatatgact ccttatcgtg aacgtaaggt
    18421 gcatttgctt aatggaccgc atacagcgat ggtccctctt gctttgttgg cggaattgga
    18481 aactgtagaa gaagtaatga aagatccttt attctccgca tatgttgatc agttattcaa
    18541 tctagaattg attcctatgc tttctttacc taaagatgaa ttagccattt atgctgacca
    18601 aatcaaagaa cgtttcttga atccttttgc ccatcacaaa ttagaagcga tttcattaaa
    18661 tagtgtttca aagttcagca cccgtctgct tccagtgttc aagaagtata tcgaagaaca
    18721 aaatcaggta ccaccgttaa tcactgtttc actagctgcg ctacttctta tgtatcgagg
    18781 cgatcaagta aagccacatg atgatgaaaa gacgataagc gaatttactg atgcttggtc
    18841 tgataatgga acagctattc ctcgattatt gcaaaacgct gcgctttggg gagaagattt
    18901 gtctcaaata ccgaatgtga cagatacagt tcaagaaata gctgcaaaga tcgatcaaaa
    18961 aggtgtccgg tcagtattac aagaaatgaa aggaacgatg gtccatgctt gaaaccctgc
    19021 aaccaacaat gaaattaaat cgacgagaca gtgtggtcgt cgcattaacc cctattccaa
    19081 aacaaacaca actgacgatt gatcagcaaa cgatcaccac tcttgaagat atcccgcaag
    19141 gtcataagat tgcattgggt gatttgaaag ctggagacaa cgtgatcaaa tatggctatc
    19201 caatcggtca tgtgacgaca gacgtaactg ccggtcaatg gctgcacact cataatgtaa
    19261 aaaccaatct ttctggtgaa ttggattatc gatatgaaaa agatgtccat ccaagtcatt
    19321 acctttttga gaaccgaaca tttcaaggtt acttgcgaaa aaatggaaaa gtagggattc
    19381 gtaatgattt gtttttagta cctaccgttg ggtgtgtgaa tggcattgct gaactgatcg
    19441 tcaaacagtt caaggaaaaa catccagatc tgggacagtt tgaccatatt acgattttaa
    19501 agcatcctta tggctgttcg cagcttggaa aagatcatca aaatactcga gaaattttag
    19561 cggacgcagt aaatcatccc aatgccggag gagttttagt atttggatta ggttgtgaga
    19621 acaatacagt tcctgaattc aaaaaaattt taggtgaata tgatgaagaa cgagtgaaat
    19681 ttctagtggc tcaagatgtt tacgatgaga tcgaacaagg tgtggctcta ttagaagagc
    19741 ttttaacagc ggcggaaaat gaccagcgca tatcagtccc gctctctaag ttgaatgtcg
    19801 gtttgaaatg cggcggttct gatggtctat cgggaataac tgctaatcct ttacttggtg
    19861 cattttctga ctacttgatt gctcaaggag gcagcacgat cttaacagaa gtgccagaaa
    19921 tgtttggcgc agaacaagta ttgatggcac gagcggaaaa tgaagaagtt tttgactcaa
    19981 tcgttgactt gatcaatgac ttcaaacagt atttcttgtc ttacggggaa ccagtttacg
    20041 aaaacccatc tcctggtaat aaagcaggtg ggatcactac attagaagat aagtctttag
    20101 gctgcacgca aaaatcagga agctctcctg tcgttgatgt gctacaatat ggcgaaaaaa
    20161 tcagaaaacc agggcttagt ttattgcaag ctcccgggaa tgatcttgta gctgcctctg
    20221 ctcttgcttc atccgattgc cagttggtct tgtttaccac aggaagaggg actccgtttg
    20281 gctcatatgt cccaacggtc aaggtatcta cgaatacaac tctttttgaa cgcaaaggtc
    20341 attggatgga ttttaatgca ggcgtcttat tagaacaacc aatggaagta atcttagaat
    20401 cgtttgttca aaaaatcatc gctgtagcta gtggagaaga aacgaaaaat gaacaaaatg
    20461 acgttcgaga aattgccatt tttaaaaacg gagtaacact ttgattttca taaaacagta
    20521 taaaaagaaa gaaggaagat ttatgttttt atctgatgat tttttattaa aagacgattg
    20581 ggcgaaaaag ctttaccatt cctacgcaaa aaacatgccc attattgatt atcactgtca
    20641 cttagatcca aaagaaattt acgaaaacaa caatttcagc aatctgacag aagcttggtt
    20701 aggaggcgat cattacaaat ggcgcttgat gcgagcttgc ggcgtgcctg aagaaaaaat
    20761 cacaggaaat gcttctgatt ttgaaaaatt tttagcttgg tgtcaaaccg taccaaaaat
    20821 tatcggaaat ccgctttaca gttggtcaca tcttgaattg aagcgtttct tcggtattga
    20881 cttgttgata aatgaagaaa atgcagagaa aatttggaca caagcaaatg aaattttggc
    20941 agctcctgat tttcgccgtc gggaaatcgc aaaaaaatcg aatgtagaag tgatttgtac
    21001 aacagatgat cccgcagacg acttgcacta tcatcagttg cttgctcatg aagaaccaga
    21061 tttgaaggtt ctaccttcat tccgtccaga taaagcactc aatattgaaa aagaaggatt
    21121 taatgcatgg gtcacactac tggaagaagc agctgaccaa acgatcacca cctatcaaga
    21181 actgatagaa gtcttagggc agcggataga atacttccat caacatggat gtcgtgtttc
    21241 cgaccacgct ttagacatct tacgctataa agaagcggat gaaacagtat tagaagagat
    21301 ttttcaaaaa gcacgagcta acaaactatt atcttcagat gaaatcgatg cttatcttac
    21361 agaaacactg atacatttga cccacttcta tcattctcat aattggacga tgcagctgca
    21421 catccatgct tatcgtaact gtaatacaca aatgttggaa aaattagggc ccgataccgg
    21481 ctatgatgga atgaatgatc tttctttgac tattcctctg caaaaattat tgaaccgtgc
    21541 agaagaaacg gatcagttgc caaagacgat cctttattca ttgaatccga atgactatcc
    21601 tgcgatttta gctttgatgg gctgtttcca aaaagaacag aatggaaaac tgcagttagg
    21661 ttctggatgg tggtacaacg atactcgagc tggtatgcgc gatcaattga cacaatttgc
    21721 tgatggtagt gctttgggga attttgtcgg catgctgaca gattcaagaa gtttcctgtc
    21781 ctacactaga catgaatatt tccgtcgtgt tctttgtgaa tttttaggcg aaatggtcat
    21841 gcgaggagaa gcacctgaag atgaacaact tttaggaagt ctagttcaag cgattagtta
    21901 tacaaatgct aaaaattact ttggcttttt tgcctaattg attaatagta acagtaacga
    21961 atcaaaaaca ggatagtgaa ataacggaat ccgtttatcc acgttttaga gaagaagacg
    22021 gaccaa
//
LOCUS       DACEEZ010000043        21912 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20313_2017
            SAMN12639966-rid9879223.denovo.043, whole genome shotgun sequence.
ACCESSION   DACEEZ010000043 DACEEZ010000000
VERSION     DACEEZ010000043.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639966
            Sequence Read Archive: SRR10023158
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 21912)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21912)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741683.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741683.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:44:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,016
            CDSs (total)                      :: 2,962
            Genes (coding)                    :: 2,889
            CDSs (with protein)               :: 2,889
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 36 of 73
            Pseudo Genes (incomplete)         :: 29 of 73
            Pseudo Genes (internal stop)      :: 18 of 73
            Pseudo Genes (multiple problems)  :: 8 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21912
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639966-rid9879223.denovo.043"
                     /isolate="E20313_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(459..1088)
                     /gene="udk"
                     /locus_tag="IWO89_001634"
     CDS             complement(459..1088)
                     /gene="udk"
                     /locus_tag="IWO89_001634"
                     /EC_number="2.7.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uridine kinase"
                     /protein_id="HAQ0456382.1"
                     /translation="MTKSKPIIIGVTGGSGSGKTSVSRAIFNNFPDHSIMMLEQDSYY
                     KDQSHLSFEERLNTNYDHPFAFDNDLLIQHVGDLLNYKAIEKPVYDYVAHTRSQATII
                     QEPKEVIILEGILILEDERLRDLMDIKVYVDTDDDIRIIRRIKRDMEERGRTLDSVIE
                     QYLTVVKPMYHQFIEPTKRYADIIVPEGGENHVAIDLITTKVASFLNHK"
     gene            complement(1172..1867)
                     /locus_tag="IWO89_001635"
     CDS             complement(1172..1867)
                     /locus_tag="IWO89_001635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="noncanonical pyrimidine nucleotidase, YjjG
                     family"
                     /protein_id="HAQ0456383.1"
                     /translation="MKYKTLLFDVDDTLLDFQLTEANALSALFAEQGITLTPEIETSY
                     KALNHHLWQEFEKGRLSREEIIGSRFGLLFQQFGKTVDSDQMEKRYRHYLNQGHDLVP
                     GSLEILKKVHPHADLYIVTNGVSHTQHRRLTDAGMLDYFKDIFVSEAVGAQKPMKEFF
                     DHVFSHIPNFDKKQTVIIGDSLTSDIKGGNMAGIDTIWFNRNKLPEIEEIQPTYRIDS
                     LEELYSLLEIEKD"
     gene            complement(1880..2596)
                     /locus_tag="IWO89_001636"
     CDS             complement(1880..2596)
                     /locus_tag="IWO89_001636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HAQ0456384.1"
                     /translation="MRLDKLIEEKLETTRKEMKRLFALKQVMIDGTVEFRQNRNVDSL
                     LHQIEVAGEHLETNESYYLLNKPEKAVTAVSDPEKTTVIDLIASADRFQPLYPVGRLD
                     RDTTGLLLITSNGQLGYEMLLPEKKVSKTYQVVVNERVTPADVTAFENGIVFADGYQC
                     KPADLKILKADKHQSTVLLTIQEGKFHQVKKMFLAQGKKVTALKRLSMGPLELDEQLG
                     VGEYRSLTLAEMKKLSIYFK"
     gene            2926..3534
                     /locus_tag="IWO89_001637"
     CDS             2926..3534
                     /locus_tag="IWO89_001637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L25/general stress protein
                     Ctc"
                     /protein_id="HAQ0456385.1"
                     /translation="MSVSLEVSKREVRPRSLRNKLRHEGKVPAVVYGYKVESTPISFD
                     GLTLSKLLREHGANTVITMDIDGQKVNTLVHKIQTNTFTNHFEHAEFLSVNMSEETEV
                     ETEVVLVGESVGAKSGGVLAQNLYTVVVSATPDKLPERIEVDVTNLELGDSITVADLP
                     KNDDYKFVTDSEEQIAAVVAQSEEEESTGEGGEPEVIGSTEE"
     gene            3859..4182
                     /locus_tag="IWO89_001638"
     CDS             3859..4182
                     /locus_tag="IWO89_001638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF960 domain-containing protein"
                     /protein_id="HAQ0456386.1"
                     /translation="MFESFDSSRNRYASVGVVSSLPDELIDSIWFIIDLDLKGVIPLE
                     NILTFDLIENRGKVTMHFSQAGSNVEMDIDLPFAYSNQFPAQVYAYDDGTRETILLPS
                     EIRQY"
     gene            4364..5734
                     /locus_tag="IWO89_001639"
     CDS             4364..5734
                     /locus_tag="IWO89_001639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HAQ0456387.1"
                     /translation="MRDLTKGSPAKLILMFTVPLLIGNVFQQFYNMIDMIIVGQTLGK
                     NALAAVGATGSLTFLIIGFAQGLTAGLAIITAQRYGAKDYRGLKKSFAASVVISLVVT
                     VILTVLSLLFIRPMLQLMQTPPEILDQAQTFISIILLGIFASVSFNLLSNVIRALGDS
                     RTPLFFLIIAVIINVVLDLIFIIYFGMGVEGAAIATVIAQVSSSVLCLVYIKKKMPLL
                     QLRKKDFKFEKEEFAVHLNAALPMAFQSSIIAVGAIVLQAALNSLGTDVVAAQAAAGR
                     IDQFANQPMMSFGIAMATFTAQNYGAKEYGRILQGVRQTLLMSVGFSLLAGAIVIFFG
                     HFFVQLFVSPSETRVFELAQTYFNINGSLYWILAVLFILRYTLQGLGQSKIPTIAGMM
                     ELLMRSFAAIVLTGMWGYPGAAAASPLAWIGSVAVLLYSYMRSMKQLKHMGEEEHFTL
                     EHAEAR"
     gene            complement(5864..7165)
                     /locus_tag="IWO89_001640"
     CDS             complement(5864..7165)
                     /locus_tag="IWO89_001640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyC/CorC family transporter"
                     /protein_id="HAQ0456388.1"
                     /translation="MVINFIILFLMIAITSFFVASEFALVKIRRSRLEQLEKENVKNA
                     KLALHVTHHLDNYLSASQLGITLTGLIIGWVGEGSVAALLEPVIGKLPFSSAISSTIS
                     VALGFILVTYIDVVIGELLPKSYSIVNTEKVVLFVVKPLHYFYKVMFPFIWVLNHSAA
                     GLGKLLGVRLVSEGEETLSQEELTLVALNSYEKGELTKEEYYYLENVFEFDDTLAKDI
                     QVDRTSMQVFEASETVKEAIKASIEQGHTRYPVILESKDNVLGYVTLPDLIKQSYKDD
                     QLTVEQLIEEPIVTTETIPIKKLLTIMRKKGKHIAILKDEYGGTSGLVTIEDILEEIV
                     GEIRDETDLDEALIAEQSDGSYIISGKLTLDDFQRYFHVEIPEFEETNFTTLAGFASS
                     RYKEIKAGTIIEIASFRFTVLEYQHAHIDYFKVESTERKTE"
     gene            complement(7519..9510)
                     /locus_tag="IWO89_001641"
     CDS             complement(7519..9510)
                     /locus_tag="IWO89_001641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium transporter Kup"
                     /protein_id="HAQ0456389.1"
                     /translation="MATTHNTKKISMTGLLVAMGVVYGDIGTSPLYVMKSIVEDNGGL
                     SGINPDFVIGSVSLIFWTLTLLTTIKYVVIALNADNHGEGGIFSLYTLVRKGGKYLII
                     PAMIGGAALLADGVLTPAVTVTTAIEGLRGIPQFFDRFGNNQNVIVIITLVIIFVLFM
                     VQRFGTDIVGKAFGPIMFAWFTFLGVMGLMNFSQDWTVIRALNPYYAIQLLFSADNKL
                     GLFILGNIFLATTGAEALYSDLGHVGKRNIRLSWPYVKICLVINYFGQAAWLLNVYQN
                     PEAQQIKNLNPFFQMMPQSWIVIGVAFATVAAVIASQALITGSFTLVSEAIKLKLLPR
                     LKIMYPGNSIGQMYIPAVNLILWIACSLVVVTFRTSHHMEAAYGLSITVTMLMTTALL
                     YFYLIQNGHSRWLAYLVTFFFGAIEVVFFISSVVKFFRGGFVAVLIGLIILVVMFIWE
                     QGNIIRESSTEDVSLRDYIPQLRELRDDTSLPIYQTNVVFLVPDMAEDKVGRQFIYSI
                     LDKRPKRARVYWFVHVEVTDEPYTKEYQVDMMDTDFIVQVNLFLGFRVQQEINVYVRQ
                     VIHDLMKQGRLPKQPQRYSLTPGREVGDFQFVLIDEVVSNVTTLGKWQRQIMQAKLAI
                     KKIATSPETWFGLEYSEVKHETVPLIIGNTRKTWLKERL"
     gene            complement(9654..9920)
                     /locus_tag="IWO89_001642"
     CDS             complement(9654..9920)
                     /locus_tag="IWO89_001642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HAQ0456390.1"
                     /translation="MLNFLWSLFIGGILGLIAGAILGKEVPGGVSGNIIIGFLGSWLG
                     EFLLGPLGPELGGFYLIPALLGAIFCLALYSFVARRIRERPKKE"
     gene            complement(10430..12556)
                     /gene="pnp"
                     /locus_tag="IWO89_001643"
     CDS             complement(10430..12556)
                     /gene="pnp"
                     /locus_tag="IWO89_001643"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HAQ0456391.1"
                     /translation="MSEKQVFKTTWGGRPLQIEVGQLAKQANGAVLVRYGDTVVLSAA
                     VASKEAKDTDFFPLTINYEEKMYAAGKIPGGFIKREGRPSTEATLTARLIDRPIRPMF
                     AEGFRNEVQVTNIVMSVETDCSPAMAAMLGSSLALSISDIPFDGPIAGVEVGRVNGEY
                     VLNPTVEQAEQTDIELTVAGTKQAINMVESGAKEVSEEDMLGALLFGFDAIKELVAFQ
                     EEIVQAVGKEKMEVTLLQVDEVLKKEIFDASYATMKAAVMTEEKLAREDNIEQVKIDI
                     REAYAEKFAGHEDEDHLLKEVKQITEDLEKDVVRELITIDKIRPDGRKLDEIRPLSSE
                     VSLLPRVHGSGLFTRGQTQALSACTLAPLGEHQIIDGLGVEVSKRFIHHYNFPQFSVG
                     STGRAGSPGRREIGHGALGERALAQVIPSEEEFPYTIRLVAEVLESNGSSSQASICAG
                     TLALMDAGVPIKAPVAGIAMGLVSDGENYTILTDIQGLEDHLGDMDFKVAGTKDGITA
                     LQMDIKIQGITEQILTEALTQAKQARMEILEELTSTIAAPREELSQYAPKIEMIQIEP
                     AKIKDVIGKGGDTINGIIDETGVKIDIDQDGKVSIASADAEMIKKAIKIIEDLTKEVK
                     VGEVYLGKVVRIEKFGAFVNLIKGKDGLVHISQLANDRVNKVEDVVKLGDEILVKVTE
                     IDKQGRVNLSRKAMLNEDGSTKNEEK"
     gene            complement(12744..13013)
                     /gene="rpsO"
                     /locus_tag="IWO89_001644"
     CDS             complement(12744..13013)
                     /gene="rpsO"
                     /locus_tag="IWO89_001644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HAQ0456392.1"
                     /translation="MAISKERKNEIINEYARHEGDTGSPEVQIAVLTEEINHLNEHAR
                     VHKKDHHSYRGLMKKVGHRRNLLAYLRKTDVQRYRELIKRLGLRR"
     gene            13264..13827
                     /locus_tag="IWO89_001645"
     CDS             13264..13827
                     /locus_tag="IWO89_001645"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HAQ0456393.1"
                     /translation="MITMDDIIREGNPTLREVAKEVSLPLSEEDISLGKEMLEFLKNS
                     QDPIKAEELHLRGGVGLAAPQLDISKRIIAVHVPSPDPEADGPSLSTVMYNPKILSHS
                     VQDACLGEGEGCLSVDREVPGYVVRHAKITVSYYDMNGEKHKIRLKNYESIVVQHEID
                     HINGVMFYDHINDQNPFALKEGVLVIE"
     gene            complement(14080..14601)
                     /locus_tag="IWO89_001646"
     CDS             complement(14080..14601)
                     /locus_tag="IWO89_001646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription repressor NadR"
                     /protein_id="HAQ0456394.1"
                     /translation="MIEGETRRKQIMQTLANTEKPVSASKFAKTFGVSRQIIVGDIAL
                     LRAAGKSIVATARGYILETEETNTGHISKIAVQHGKDQTEEELRLIVENGGEIIDVIV
                     EHPLYGELAGTLHIKTPQDIDSFMRRYQKSNATLLSELTGGIHLHTIRYPEKHMLKNI
                     KQKLAEAGILYEG"
     gene            complement(14604..15446)
                     /locus_tag="IWO89_001647"
     CDS             complement(14604..15446)
                     /locus_tag="IWO89_001647"
                     /EC_number="5.4.99.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate mutase"
                     /protein_id="HAQ0456395.1"
                     /translation="MTEFKTEKEKMIAGELYFANDPELVADRMFARSQSQIINQAESA
                     ELRSQLLKETFGRTGKKIYMEPVINFDYGYNIFVGENFYANFNCTFLDVSTIEIGDNC
                     MFAPNVQLYTATHPLHPVKRNSGLEYAKPIKIGNNVWLGGGVIVTPGVTLGDNVVVGA
                     GSVVTKSFPDNVVIAGNPARIIKTVEEELTEESLETLRHKIDMIDRELVALLEKRMDT
                     VTKIGQVKKEENQAVYDAKREQQVLDKVVSLLKNKGYKETITDTYVDLMKHSREYQNK
                     MKEE"
     gene            complement(15439..16164)
                     /locus_tag="IWO89_001648"
     CDS             complement(15439..16164)
                     /locus_tag="IWO89_001648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent protein deacylase"
                     /protein_id="HAQ0456396.1"
                     /translation="MKQLTVDEAAKEILAYQQITFLTGAGVSTPSGVPDYRSLSGIYH
                     GMEQPEYLLSHQAMVNEPDKFYQFVKKIYHPEAKPNIIHLEIAHLAKEKNVWVVSQNI
                     DGLHAKAKSPQLVNFHGSLYQCHCRKCQQTVDWKEYLHSDKHQACGGQIRPDIVLYGE
                     GFQDGVMEQAAYAVSQAELIVIVGTSFQVHPFCDLVQFRQPSAKILVINQTPVYLAEP
                     YTFIQEDGAVVFKKIQELGADYD"
     gene            16340..16636
                     /locus_tag="IWO89_001649"
     CDS             16340..16636
                     /locus_tag="IWO89_001649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0456397.1"
                     /translation="MLPFFIYWGVILACIAWLALSVYFSVFYLVRKENGNLWAFALFN
                     VIAAIVLAITLAVYRTWGWGITQYSSLIYLILAIYGVVVLLQAILGREPKKAAA"
     gene            17204..18796
                     /locus_tag="IWO89_001650"
     CDS             17204..18796
                     /locus_tag="IWO89_001650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750719.1"
                     /note="The N-terminal region of this protein, as described
                     by TIGR01726, is a three transmembrane segment that
                     identifies a subfamily of ABC transporter permease
                     subunits, which specificities that include histidine,
                     arginine, glutamine, glutamate, L-cystine (sic), the
                     opines (in Agrobacterium) octopine and nopaline, etc;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HAQ0456398.1"
                     /translation="MKKNMVSVAVLFAFVFSLFSVGSLANADEKTPSDEFRVGMEAGY
                     APFNWSQQTDENNALPIQGQNSYAGGYDVQIAKKVADGLGKKLVIVQTKWDGLAPALQ
                     SGKIDAIIAGMSPTAERRKEIDFTDPYYESQLVVVVQKDSKYAKAKSLADLSGAKITA
                     QLNTFHYTVIDQIPDVQKQQAMDNFSAMRTALASGMIDGYVSERPEGVTATSVNKDLE
                     MLEFSGADGFQTNPEDVQISVGMRKGDPDIQKVNQILSGISNDERTKIMDQAVKDQPA
                     ATDSEEKPGIISDFTTIWKQYGNMFLRGAGLTLFIALIGTVVGTTLGLLIGVFRSVPE
                     SGNALTRFFQKIGNALLSIYVEVFRGTPMMVQAMVIFYGLALAFGISLNRTIAALFIV
                     SLNTGAYMTEIVRGGIFAVDEGQFEAAQAIGMTHGQTMRKVVIPQVLRNILPATGNEF
                     VINIKDTAVLSVIGVADLFFQGNSASGANFQFFQTFTIVGIIYLVMTFTITRILRAVE
                     KKIDGPSAYTKLEEIDNSNLQE"
     gene            18809..19549
                     /locus_tag="IWO89_001651"
     CDS             18809..19549
                     /locus_tag="IWO89_001651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HAQ0456399.1"
                     /translation="MNEKIIEIKHLRKSFGENEVLKDISVTVNKGEVVTIIGSSGSGK
                     STFLRCINLLEKPTGGEIIYNGENVLAPKYNLPKYRTNLGMVFQSFNLFNNMNVLENC
                     MSGQTTVLKRDKETAKKVAIENLEKVGMDRYIEAKPAQLSGGQKQRVAIARALSMNPD
                     VLLFDEPTSALDPEMVGEVLNTIKDLAHTGLTMLIVTHEMEFAKDVSDRVIFMDKGVI
                     AEEGTPEDIFVHPKEERTKEFLSRILNA"
     gene            19717..21066
                     /locus_tag="IWO89_001652"
     CDS             19717..21066
                     /locus_tag="IWO89_001652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAQ0456400.1"
                     /translation="MNEEQELQRGLKNRHVQLISIGGAIGTGLFLGSGKSIQLAGPSI
                     LLAYLITGCICFFIMRALGELLLSNTNNHSFLDFVAEYLGKKAAFITGWTYWFCWVSI
                     AMADLTAIGMYVRYWAPGVPQWLPELIALGLLLCLNMVAVSLFGELEFWFALIKVVAI
                     IAFILVGAYMILTHYTTDIGTASITNLWSHGGFFPTGAKGFILAFQMVTFAFAGIELV
                     GLVAGETENPEKVLPEAINNIPIRIILFYLGSLFVIMAIYPWNSLNPDNSPFVEVFSE
                     IGITIAASLINLVVLSAAASACNSAIYSTGRMLRSLAQEGSAPKKFKKLTTHHVPGNA
                     LTFSTIVIFISVILNYVMPSEVFTLVSSIATTCFLFIWSMLVYTHMKYRKSILGKKAH
                     SFKMPLYPFSNYLVFLYMAFVCVVLFLGKDTRIALLLTPVWFLLLLLIYHMKYERKK"
     gene            complement(21194..21784)
                     /locus_tag="IWO89_001653"
     CDS             complement(21194..21784)
                     /locus_tag="IWO89_001653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1211 domain-containing protein"
                     /protein_id="HAQ0456401.1"
                     /translation="MTKSRLEAFTDGVVAIVLRVIVLEIKIPNTSSFHSLWVIRNTLL
                     AYTISFIFVAVIWVNHHRIFQMAERINYRVVWSNIFLLFWLTLCPSVTSWVGRNPEAF
                     WPEMAYVLVYTMWSFSFGILMRQIMKANKPDSHVVRVLSKDRRSLISMMINLCLIAGV
                     FFVPLIGLFGRFFVSGIWIFSYKKADAYYQRLFPGK"
BASE COUNT         6295 a         4462 c         4158 g         6997 t
ORIGIN      
        1 gggggtgcga caaaagagct tcttcaaaaa aataagtcga aatctcacac aaattcgaga
       61 aataattttc gtaagtttct tcttatttat tgcatctgac cacttctgtt acaacctctc
      121 gttaaacggt gttcaaaagc ttgcatctac gataataagc ccaacccaac gaaaaacgtg
      181 aggagctgtt ttatttggat gctcttatcg cttgatgcaa agacagcttt tttacaacct
      241 ctacttcacg gtgctcaatc agctgacctt cggtattagc tcaaaaaacg gtaaaatata
      301 aacagctatt ttccgtttct tctttttaat acttaggtca agatgctgtt ttcacaacct
      361 cttgttataa ataaaaaaac agttggctgg tgactgttca aaaagcagaa tccttgcttt
      421 tcaaacggtc gcagtccgtt caactgtttc gctgcgcact atttatggtt taagaaacta
      481 gctacttttg ttgtaatcaa atcaatagca acgtggtttt ctccgccttc tggaacaatg
      541 atatccgcat agcgcttcgt tggttcgata aattgatgat acatcggttt aacaactgtc
      601 aaatattgtt cgatcacgga gtccaatgtg cgtccccgtt cttccatatc ccgtttgata
      661 cgacgaatga tacgaatgtc atcatctgta tctacgtata ctttgatatc catcaagtca
      721 cgcagacgtt cgtcttctaa aatcaggatt ccttctagaa tgatcacttc ttttggttcc
      781 tgaatgatcg ttgcctgact tcttgtatgc gctacatagt catacacggg tttttcaatg
      841 gctttatagt tcagtaagtc gcctacatgc tgaatcagca gatcattatc aaaggcaaat
      901 gggtgatcgt aatttgtgtt caatcgttct tcaaaactca aatggctctg atccttgtaa
      961 taagaatctt gttcaagcat catgatggaa tggtccggga agttgttgaa aatcgcacgg
     1021 ctgacacttg tctttccgct tccggatcca cccgtcacac caatgataat tggcttgctt
     1081 tttgtcattg actaaaacct ctttcgtatc cgttttttca ttctaatcta ttatagcgaa
     1141 aaaacacgaa aatgctatat ttttctgaaa attaatcctt ttcaatttcc aataatgaat
     1201 aaagttcctc taatgaatcg attcgataag taggctggat ttcttcgatt tctggtaatt
     1261 tatttctatt gaaccaaatc gtatcgatcc ctgccatatt tcctcctttg atatcagaag
     1321 tcaaagaatc accaatgatc acagtttgct ttttatcaaa attaggaatg tgggaaaaga
     1381 catgatcaaa aaattctttc atcggctttt gcgctccgac agcttctgag acaaagatat
     1441 ccttgaagta atcaagcatt cctgcatctg ttaatcgccg atgttgcgtg tgcgacacac
     1501 cgttcgtcac aatataaaga tcagcatgcg gatggacctt tttcaggatt tctagacttc
     1561 ctggaacaag atcatgacct tggttcaagt agtggcggta gcgtttttcc atttgatcac
     1621 tatctactgt tttcccaaat tgctggaaaa gtaacccaaa acgactgcct attatttctt
     1681 ctcgtgatag tctgcctttt tcaaactcct gccataaatg atgattcaaa gccttatagc
     1741 tggtttcgat ttctggtgtc aatgtgattc cttgttctgc aaacaaagca cttaaagcat
     1801 tggcttcagt taattgaaaa tctaacaatg tatcatctac atcaaacaat aaagttttat
     1861 atttcatctt tttgacctcc tatttaaaat aaatacttaa ctttttcatc tctgctaatg
     1921 tcaacgatcg atactcgcct actcctagct gttcatctaa ttccaatggt cccatgctta
     1981 gccgtttcaa tgcggtcact ttctttcctt gggctaagaa catctttttg acttgatgga
     2041 attttccttc ttggatcgtc aataaaaccg tactttggtg cttgtctgct ttgagtattt
     2101 ttaaatcagc cggcttacat tgatacccat cagcgaatac gattccattt tcaaatgctg
     2161 ttacgtctgc cggtgtcacc ctttcgttca caactacttg ataagtcttt gaaacctttt
     2221 tttctggaag tagcatttca tatcccaatt gtccattcga agtgatcagc aatagtcctg
     2281 ttgtatctct atctagtctt cccactggat ataatggttg gaaacgatcg gcacttgcaa
     2341 tcagatcgat tactgtcgtc ttttctggat ctgatacagc tgtaacagct ttttcaggtt
     2401 tattgagaag ataataggac tcatttgttt ctaagtgttc acctgctact tcgatttgat
     2461 gcagtaaact atctacattt cgattttgtc gaaattcgac tgtgccatct atcatcactt
     2521 gcttcaaagc aaacagccgc ttcatttcct tgcgtgtcgt ttctaatttt tcttctatta
     2581 atttatctaa acgcatgctg tttcctttct tattactact aattaattat accttgaata
     2641 ttcttgcttg caaatacatc atcatagtta ttccttgact taattaacag ttcacgttac
     2701 ggttaaatag taaagtgaat tggatttctc atacacagcg taatgacctg gtggagaaaa
     2761 gttgaatcct gactttctaa ggggagatgc gcgcatctct ccttttgcgc ttttttaagc
     2821 gccctgcctt cgatgatgtg cacgagatta gcctttagaa ttcacttaaa taaaatctgt
     2881 ccgttaccaa agcagacagt cacattttaa ggaggaattt tggttatgtc agtatcatta
     2941 gaagtaagta aaagagaagt acgtccccgt tcattaagaa acaaacttcg tcacgaaggg
     3001 aaagttccag ctgttgttta tgggtataaa gtagaaagta cccctatctc attcgatggt
     3061 ttgacactaa gcaaattgtt gcgtgaacat ggagcgaaca cagtcatcac gatggacatc
     3121 gatggacaaa aagtcaatac attggttcac aagatccaaa cgaacacttt taccaatcat
     3181 ttcgaacatg cagaatttct atctgtaaac atgtcggaag aaacagaagt cgaaacagaa
     3241 gtcgttcttg ttggcgaatc agtgggtgct aaatctggtg gagtgttagc tcaaaacttg
     3301 tatacagtag tcgtatctgc tacaccagac aaattgcctg aacgaattga agttgacgtg
     3361 acaaacttgg aattaggtga ttctattaca gtagccgatc ttccaaaaaa cgacgattac
     3421 aaattcgtga ccgacagtga agagcaaatc gctgctgtag tcgctcaaag tgaagaagaa
     3481 gaaagcacag gtgaaggtgg cgaaccagaa gtgatcggtt caacagaaga ataatactga
     3541 aacaatcagg tggtgttaga agcggcgggt cagcagcttt taacacgcct tttttatttc
     3601 taatcgttta ttttcatatg gggcttccac ttttgtaaat ggctttacaa acgaaaaatc
     3661 ctaatttacc aagcttttcc tcgcatactt ttatcgtacc tttataaaaa tttatacttt
     3721 gaaaaaaagt attcaattat tgttgcattt ttcctgattc tttattagaa tgaaccctcg
     3781 ttgaagtaag tatttctagt ttatcctagg attaattata aaaataaaac attcgttgat
     3841 tcgaaggagg ttaaacagat gtttgaaagt tttgatagca gccgcaaccg ttacgcttca
     3901 gttggtgtcg tatcaagttt gccagatgaa ttgattgaca gtatatggtt tattattgac
     3961 ctagatctta agggagtcat tccattagaa aatatattaa cctttgattt gatcgaaaat
     4021 cgtggaaaag tgacgatgca tttttctcaa gcaggaagca atgtggaaat ggatattgat
     4081 ttgccgtttg cctattcgaa ccaatttcct gctcaagtat acgcctatga tgacggtacg
     4141 agagaaacga tccttttacc aagtgagatt agacaatatt aaggtgatag ataaacgtgg
     4201 atgtccctta ttttacagac caggccaagt tgttgattct ttgactcaat tcttggcttc
     4261 tttttatgga gtttcataaa ttatcattga tttctaattg gtattctcag gataaacgct
     4321 atatactact aactaaattt taaagatata aggagtttaa aagatgcgcg atttaacaaa
     4381 aggaagcccc gctaaactga ttttgatgtt cactgttcct ttactgatcg gaaatgtttt
     4441 tcagcagttc tacaatatga tcgatatgat catcgtgggt cagacgctag gtaaaaatgc
     4501 gctggcagcg gttggtgcaa ctggaagcct gacatttttg atcattggct ttgcccaagg
     4561 tttaacagct ggtttagcca ttattaccgc acaacgttac ggtgcaaaag attatcgggg
     4621 tctgaagaaa agttttgctg ctagtgttgt cattagtctt gtcgtcactg tgattttaac
     4681 tgtcctaagt ctcctgttta ttcgtccgat gcttcaattg atgcaaactc ctcctgaaat
     4741 acttgatcaa gcacaaacat ttatttcaat tattttatta gggatctttg catcagtcag
     4801 tttcaatttg ctttctaatg tcattcgcgc acttggtgac agccgcacac cactattctt
     4861 tctgatcatt gcggtcatca tcaacgtcgt acttgatttg atctttatta tctattttgg
     4921 catgggtgtg gaaggtgcag ctattgctac agtaatcgca caagtttctt ccagcgtact
     4981 gtgtctagtg tatatcaaga aaaaaatgcc tttgttacag ttacgcaaaa aagatttcaa
     5041 atttgaaaaa gaagagtttg ctgtacacct aaatgctgct ttgccaatgg cgtttcaatc
     5101 ttcgatcatc gctgttggtg ctatcgtgtt acaagcagca ctaaatagtc tagggacaga
     5161 tgtcgtagct gctcaagccg ctgctggtcg tatcgaccaa tttgcaaatc agccgatgat
     5221 gtcatttggt attgcaatgg caactttcac tgcacaaaac tatggtgcga aagaatatgg
     5281 ccgaatcttg caaggggtac gtcaaacttt actgatgagt gtcggattta gtctcttagc
     5341 aggtgctatc gtcattttct tcggtcattt ctttgttcaa ttgtttgtta gcccatcaga
     5401 aacacgtgta tttgaattag cacaaaccta cttcaatatc aatgggtctc tttactggat
     5461 cttagcagtc ctgttcattt tacgctacac acttcaaggg cttggacaaa gcaagatccc
     5521 tacgatcgcc ggtatgatgg aattgcttat gcgttctttt gcagctatcg ttcttacagg
     5581 aatgtgggga tacccaggag cagcagcagc ttctccttta gcttggatcg gttctgttgc
     5641 cgtgttgctt tactcttata tgcgttcgat gaaacaattg aaacacatgg gtgaagagga
     5701 acatttcaca ttagaacatg cagaagcaag atagccttga ttaaaagaaa tcgttgcggc
     5761 taaaaatgaa aacttttatt gaaaatatct tcttcgttta ccgaaaacag gttttttaga
     5821 taacaaaaag gctgtgacaa aaaatgtcac agcctttttt cttttattct gtttttcttt
     5881 ccgtggactc tactttgaaa taatcgatat gtgcatgctg gtattctaat accgtgaaac
     5941 ggaatgatgc aatctcaatg attgttcctg cttttatttc tttatatcgg ctagaagcaa
     6001 agcccgcaag tgtagtaaaa ttcgtttctt caaactctgg aatctctaca tgaaaataac
     6061 gctggaaatc atctaatgtt agtttgccgg agatgatata agagccatct gactgttccg
     6121 ctatcaatgc ttcgtccaaa tctgtctcat ctcggatttc cccgacgatt tcttctaaaa
     6181 tatcttcaat cgtcactaag ccgcttgtcc ctccgtattc atcttttaag atagcaatat
     6241 gcttaccctt ttttcgcatg atcgtcaata atttttttat agggatcgtt tcagttgtta
     6301 cgatcggctc ttcaatcaac tgttctactg tcagctggtc atctttataa gattgcttga
     6361 tcaaatctgg caaggtgaca taacctaaaa cattgtcttt tgattctaag ataaccggat
     6421 agcgagtatg tccttgttca atcgaagctt ttatcgcttc tttgacggtt tctgaagctt
     6481 cgaatacttg catggacgta cggtctactt gtatatcttt tgccaaggta tcatcaaatt
     6541 caaacacgtt ttccaaataa taatattctt ctttggtcaa ctctcctttt tcataagaat
     6601 tcaatgcgac taatgtcagc tcttcttgag acaatgtctc ttctccttca gatacgagac
     6661 gcacacctaa tagcttccct agccctgcag ctgaatgatt caatacccaa ataaaaggaa
     6721 acattacttt ataaaaataa tgaagtggtt taactacgaa caagacgacc ttctccgtat
     6781 tgacgatact atagctctta ggaagcagtt cgccaataac cacatcgata tacgtaacta
     6841 aaataaatcc cagcgccaca gagattgtac tgctaatagc cgaactaaaa ggtagtttac
     6901 cgatcactgg ttctagtaaa gccgcgacag atccttcacc gacccacccg atgatcaaac
     6961 cggttagcgt aattcctaat tgactagctg ataaatagtt atctaaatgg tgtgtaacat
     7021 gaagagctag cttggcgttc tttacatttt ccttttctag ttgttccaat cttgatcgtc
     7081 tgattttcac taaagcaaat tcacttgcta caaaaaatga agtaattgca atcatcaaaa
     7141 ataaaataat gaaattgata accatattct cccgcctttc tctttttcta ttgtaccctt
     7201 tttctacaaa aagattgaaa ataatgcgta ttcatattta ggaataaaaa aggagatgcg
     7261 gcatttttca gccacatctc cttatcactt caaaatcatt gcgttacttg ttgctaaaca
     7321 gctttttcat gacctctgat aaaaacggta ttcaaaagct tgcatctacg gcattaagcc
     7381 caaacagatg aaaaacatga gaagctgttt ttatctgctc gttcttactg cttgttgcag
     7441 tacagctttt tcatgacctc tgataaacgg tgttcaagta gctgacattc gacattagtt
     7501 caggtcaaag cgctactttc acaaccgctc tttcaaccat gttttccgag tatttccaat
     7561 gataagcgga acagtttcat gttttacttc gctgtactct agcccaaacc atgtttctgg
     7621 cgaggtagcg atttttttga ttgccagttt tgcctgcatg atttgacgtt gccattttcc
     7681 tagagttgtc acattggaaa ctacttcatc aatcaggaca aattggaaat cgccaacttc
     7741 tcgccctggt gtcagagagt atctttgtgg ctgttttggc aaacggcctt gtttcatcaa
     7801 atcatggatg acttggcgaa catatacgtt gatttcctgt tggaccctga aacctaagaa
     7861 cagattcact tggacaataa aatcagtgtc catcatatct acttgatatt ctttcgtata
     7921 tggctcatcc gtcacttcca catgaacaaa ccaatagact cgtgctcttt taggccgttt
     7981 atctaaaatc gaatagatga actgtcggcc aactttatct tcagccatat caggaaccaa
     8041 gaagaccaca tttgtctgat agattggtaa agaagtatca tcacgtaatt ctctaagctg
     8101 cgggatataa tctctcaaag acacatcctc ggttgacgat tcgcgaataa tgttcccttg
     8161 ttcccaaata aacatcacga ctaagatgat caaaccaatc agaacggcta caaaaccgcc
     8221 tcggaagaat ttgacaacac ttgagataaa gaaaacgact tctatcgcac cgaagaaaaa
     8281 ggtaactaaa tatgcaagcc aacgagaatg cccattttgg ataagataaa agtaaagcag
     8341 tgctgttgtc atcaacatcg tcacagtgat cgatagaccg taagcagctt ccatatgatg
     8401 agacgttcgg aatgtgacaa ccactagaga acaggcgatc cataaaatca gattgactgc
     8461 tgggatatac atttgcccga tactgttccc aggatacatg atctttaacc gaggcagcag
     8521 tttcaacttg attgcttctg aaactagcgt aaacgaacct gtaatcaatg cttgcgaagc
     8581 aatgactgca gcgactgttg caaaagcaac gccgatcacg atccaacttt gaggcatcat
     8641 ttggaagaat ggattcaggt tcttgatttg ctgtgcttca gggttttgat aaacattcaa
     8701 caaccacgca gcctgcccaa aataattgat aaccaaacaa attttgacgt aaggccagct
     8761 cagacgaata tttcgtttgc cgacatgtcc aagatcggaa taaagcgcct ctgcacctgt
     8821 cgtagccagg aaaatatttc caagaatgaa caagcctaat ttgttatctg cactaaaaag
     8881 cagctggata gcataataag gattcagtgc acggataact gtccagtctt gactgaagtt
     8941 cattaatccc attacgccta aaaaagtaaa ccaagcgaac atgattggtc cgaatgcttt
     9001 tccgacaata tctgtaccaa agcgttggac catgaataaa acaaaaataa taactaaagt
     9061 gataatgacg atcacgttct ggttattacc gaaccggtca aaaaactgcg gaattcctcg
     9121 caagccttca atcgcagtcg ttaccgtcac agcaggggtc aataccccat ctgctagtaa
     9181 ggcagctcct ccgatcattg caggaatgat caaatatttc ccaccttttc ggacaagtgt
     9241 gtagagtgag aaaattccac cttcaccatg gttatctgca ttcaatgcaa tcacgacata
     9301 tttgattgtt gtcagtagag tcaatgtcca aaaaatcaat gagactgatc cgataacaaa
     9361 atcaggattg attcctgaca atccgccatt atcttcaaca atcgatttca taacgtaaag
     9421 cggacttgtc ccgatgtctc cgtagacgac tcccatcgct acaagcaggc cagtcattga
     9481 tatctttttg gtattgtgcg ttgttgccac gtttttccct catttcttgt tatatggata
     9541 tcctttaggt aagcttgaat gatagatgat attcttatcc atgacacccg ctttcctaaa
     9601 ttgcttctaa ttatccaagt aacacactga agtgtcaact tatttgaata aaattattct
     9661 ttctttggac gttctctgat acgtcttgcc acaaatgaat aaagagcaag gcaaaaaatt
     9721 gcacccaata atgctggtat caaataaaaa ccgccgagtt ccggtcccag tggtcctagt
     9781 aaaaattcgc cgagccaact acctaaaaaa ccgataatga tatttccaga tacgcctccc
     9841 gggacttcct ttcctaaaat tgcgccagct atcaaaccta atataccgcc gataaataac
     9901 gaccataaaa aattcagcat ctcctttccc tcctttatcc atatttctca cttttatttt
     9961 acgctttatt cagaaggact ccaaaaatat gcgccaagaa actccagaaa aaaagaagtt
    10021 gtaacaaaag tgttcagctt atttccgtag aagctgcttc tggaacaccg tgaatcagag
    10081 gttgtgaaat cagcggtttg acctgagtat tagagaaaca aacggaaaaa tagttcctca
    10141 tattttgccg ttttttgagc taatatcgag ggacagctga ttgaacatcg tttattggag
    10201 aggttgtaaa aaagctgttc tgcatcaagt aataagaaca gccaaataaa aacagctcct
    10261 aatgttttta acttggctgg acttattacc gcagatgcaa gcttttgaac accgtttatt
    10321 cgaaaaaaaa ggactgtgac acggcttttg tcacagtccc cttttttcga ataattattg
    10381 tccccctgtc accttcacga tcacaagtac attattccta tgaattaaat tatttttctt
    10441 catttttcgt tgaaccatct tcgttcaaca ttgcttttct tgacagattt acgcgtcctt
    10501 gtttatcgat ttcagttact ttcacaagga tctcatcacc tagtttaaca acatcttcga
    10561 ctttattgac acgatcgttt gctaattggg aaatatggac aagtccatct tttcctttga
    10621 tcaagttgac gaatgcaccg aatttttcga tacggacaac tttccctaga taaacttcac
    10681 caactttgac ttctttggtc aaatcttcga tgatcttgat agcttttttg atcatttctg
    10741 catcagctga agcaatactt acttttccat cttgatcgat atcgattttc acaccagttt
    10801 catcgatgat tccgttgatc gtatcgccgc ctttaccgat aacatctttg attttagcag
    10861 gttcgatctg gatcatttcg atttttggtg catattggct taattcttca cgtggagcag
    10921 caatcgttga agtcaattct tctaagattt ccatacgtgc ttgttttgct tgagttaatg
    10981 cttcagttag gatttgttct gtgattcctt gaatcttgat atccatctgt aaagcagtga
    11041 taccatcttt tgtaccggca actttaaagt ccatatcgcc aagatgatct tccagccctt
    11101 ggatatcagt taggatcgta tagttttcac catcagaaac aagtcccatc gcaataccgg
    11161 cgactggcgc tttgattggt acacctgcat ccatcaatgc taatgtgcca gcacagatac
    11221 ttgcttggga agaagaacca tttgattcca atacttctgc tactaaacgg atcgtataag
    11281 gaaattcttc ttctgaaggg attacttgtg ctaacgcacg ttcacctaat gcgccatgtc
    11341 cgatttcccg acgccctgga gagcctgcac gtcctgtaga accaacagaa aattgaggga
    11401 aattgtagtg gtggatgaat cgtttgctga cttccacccc taaaccatca atgatttggt
    11461 gctctcctag aggtgcgagt gtacaagctg ataaagcttg tgtttgtcct cgtgtgaaca
    11521 aacctgatcc atgtacacgt ggcaataagc tgacctctga tgacaatgga cggatttcat
    11581 ccaatttacg cccgtcagga cggatcttat cgatggtgat caattcacgt acgacatctt
    11641 tttcaagatc ttctgtgatt tgtttaactt ctttcaacaa gtgatcttca tcttcatgac
    11701 ctgcaaattt ctcagcataa gcttcgcgga tatcgatctt cacctgctcg atattatctt
    11761 cgcgagcaag tttttcttca gtcattactg ctgctttcat tgtagcgtaa gaagcatcaa
    11821 agatttcttt tttcaatact tcatcaactt gcagtaaagt cacttccatt ttttctttcc
    11881 ctactgcttg aacgatttct tcttggaaag caactaactc tttgatagcg tcaaaaccaa
    11941 acagcaatgc accaagcatg tcttcttctg aaacttcttt cgcgccgctt tcaaccatat
    12001 tgatggcttg tttcgttccg gcaacagtca attcgatatc tgtttgttct gcttgttcta
    12061 ctgttggatt caagacgtac tcgccattta cacgaccaac ttctacgcct gcgattgggc
    12121 cgtcaaatgg gatatctgag attgatagtg ctaaagaaga acctagcatt gctgccatag
    12181 ctggcgagca gtctgtttct acactcatca cgatgttcgt tacttggact tcattacgga
    12241 agccttctgc aaacatcggg cgaattggac ggtcgatcaa acgggcagtc aatgttgctt
    12301 ccgtacttgg acggccttct cgtttgatga aacctccagg gattttccct gctgcataca
    12361 ttttttcttc ataattgatc gttaatggga aaaagtctgt atcttttgct tctttagagg
    12421 ccacagctgc acttaagact actgtatctc cataacggac taaaacggct ccgtttgctt
    12481 gttttgctag ctgaccgact tcgatttgta agggacggcc gccccatgtc gttttgaata
    12541 cttgtttttc tgacataact tctccttttc cttctcgtga aagcaagacg ctactaaaca
    12601 aatgaaaact gccgaaagcc gtcttttgag cagctttcat ttggttagta ggagtccatc
    12661 tagcttgatc acggaatttt acttaattga tatacgagta aaaaaagtga gattcatgtg
    12721 aatcccactc tttttgacta aaattaacga cgcaagccta aacgtttgat caattcgcgg
    12781 taacgttgaa cgtcagtttt acgtaagtaa gctaacaagt tacggcggtg accaactttt
    12841 ttcattaggc cgcggtatga atgatggtct tttttgtgca cgcgtgcatg ttcattcaag
    12901 tggttgattt cttcagttaa tacagcgatt tgtacttctg gagaaccagt atccccttca
    12961 tggcgagcgt attcgttgat gatttcattc ttgcgttctt ttgaaattgc catttctttc
    13021 acctctttcg attattcccc tgcgactgag taaaacgttg gtgattcgct taaccaagtg
    13081 gcaggtcatg tgcctagagc acacagctat actttacctg aaaccgggtt gaaaatcaag
    13141 tagttaatca ttaagaaaag gtagttttca aacaatctta ctactacttt tccatttttg
    13201 tggtaaacta taatgcgaaa atagacttga aacacagtct tttaagaaag gggtacatat
    13261 tccatgatta caatggatga tatcattcgt gaaggaaatc cgaccttacg cgaagttgca
    13321 aaagaagttt ctcttccatt aagcgaagaa gatatctctt taggaaaaga gatgcttgag
    13381 ttcttaaaaa acagccaaga tccaatcaaa gctgaagaac tacacttacg aggcggtgtt
    13441 ggcttagctg ctcctcaatt agatatttct aaaagaatca ttgctgttca cgtaccaagt
    13501 cctgacccag aggcagatgg accttctcta agtactgtta tgtataatcc taaaattttg
    13561 agccattctg ttcaagatgc ttgtttaggg gaaggtgaag gctgtttatc tgttgatcga
    13621 gaagtaccag gatatgttgt tcgccatgcc aaaatcactg tgtcttatta tgatatgaat
    13681 ggtgaaaaac acaagattcg tttgaagaat tatgaatcga tcgtcgttca acatgaaatc
    13741 gaccatatca acggtgtgat gttctatgac cacatcaacg atcaaaatcc atttgcacta
    13801 aaagaaggcg tattggtgat cgagtagaga attggcaggt tccttcgata ctatgctaca
    13861 agaaagcttt ataaaaaagg ggagtgctca aaagctgcac ttcccttttt tatgtttctc
    13921 cactaatcga aatttagata gacatctaga aatcgcaatt ccaaaaaatt ctgtgtattg
    13981 gatacgcatc ttttcttatt ttttcttatt tcttccatat gggtgtttat ctcagagatg
    14041 atagaatagt ttctaggata aaaagtagca gctacgatgt tatccttcgt agaggattcc
    14101 tgcttcagct agtttttgtt tgatgttttt cagcatatgc ttttctgggt agcggatcgt
    14161 atgcagatgg atgccgcctg ttaattcgga taacaaagta gcattacttt tttgataacg
    14221 tctcataaag gaatcgatat cttgtggcgt tttgatgtgc aaagttcctg ctaactcacc
    14281 gtaaagggga tgttccacaa ttacatcaat gatttcacca ccattttcaa cgattagtct
    14341 cagttcttct tctgtttgat cttttccatg ctggacagcg attttactga tgtgcccagt
    14401 attggtttct tctgtttcga ggatataacc tcttgctgtt gccacgatac ttttacctgc
    14461 agctcgtagt aaagcaatat cgcctacaat gatctgacgg ctgactccaa aagttttcgc
    14521 aaacttgcta gcactcactg gtttttctgt atttgccaag gtttgcatga tttgttttcg
    14581 ccgtgtctct ccttcgatca cacttattcc tccttcattt tgttttggta ttcgcgcgag
    14641 tgtttcatca aatcaacata cgtgtctgtt attgtttcct tatatccctt atttttcagc
    14701 agactaacaa ctttatccaa tacttgctgc tctctttttg catcataaac tgcttggttt
    14761 tcttcttttt tcacttgtcc tattttagtt accgtatcca ttcttttttc taataaagca
    14821 accaactctc gatcgatcat atcgatctta tgacgtaatg tttcaagaga ctcttctgtt
    14881 agttcttcct ctactgtctt gatgatacga gcaggatttc ctgcaatcac tacattgtcg
    14941 ggaaaagatt tggtcacaac cgaacctgcg ccgactacta cgttgtcccc tagagtgaca
    15001 cccggcgtga cgataactcc tccgcctaac caaacattat taccgatttt aatgggtttt
    15061 gcgtactcca gaccgctgtt acgtttcact ggatgtagtg gatgcgtcgc cgtgtaaagc
    15121 tggacattcg gagcgaacat gcagttatca ccgatttcaa tcgtgctgac atctaaaaat
    15181 gtacaattga aatttgcata aaaattctcc ccaacaaata tattatatcc gtaatcaaaa
    15241 ttgataactg gctccatata gatttttttc cctgtacggc caaaagtctc tttcaacaat
    15301 tgggatcgca gttccgctga ttctgcttgg ttgatgattt ggctttgact tcttgcaaac
    15361 atacggtcgg caaccagctc aggatcattg gcaaaataaa gctcgcctgc aatcattttt
    15421 tctttttcgg ttttgaattc agtcataatc tgctcctaat tcttgtattt ttttgaaaac
    15481 aaccgctccg tcttcttgaa taaacgtata aggctccgct aaataaacag gcgtttgatt
    15541 gattactaag attttggctg acggctggcg aaactgtacc agatcacaaa acggatgaac
    15601 ttgaaaactc gttccaacaa tgacgatcag ctctgcttgg ctgacggcat aagccgcctg
    15661 ctccatgact ccatcttgaa acccttcacc atataacacg atgtctgggc gtatttgtcc
    15721 tccgcatgct tgatgtttgt cgctgtgaag atattctttc cagtctaccg tttgttgaca
    15781 ttttcgacaa tgacattgat acagactccc atgaaaattg acgagttgcg ggcttttagc
    15841 ctttgcgtgc aaaccgtcga tgttttggga aacaacccaa acattttttt ctttcgctag
    15901 atgtgctatt tccaaatgaa tgatattggg ttttgcttca ggatgataaa tctttttgac
    15961 aaattgatag aatttatccg gttcattcac cattgcttga tgactcagca agtattccgg
    16021 ctgctccatc ccgtggtaga ttcctgacaa agatcgatag tctggcactc cagaaggtgt
    16081 ggatacccct gcaccggtca aaaatgtaat ttgctgataa gctaatatct cttttgctgc
    16141 ttcgtctact gttaattgtt tcatgttttg tcacctcact ttccattata gcccatactc
    16201 gaacaaattt attgttgaaa agtcagaaaa atgggaataa aaaagtgtta tgggcatttg
    16261 agcaatttat cacaatgttc actgacaaaa gaatgaaaac gtgttacaat actgctgtat
    16321 aaaagtgagg tgaatcgata tgttaccatt ctttatttat tggggcgtta ttctggcttg
    16381 tatcgcctgg ttagcgttaa gcgtttattt ctctgttttt tatcttgtac gcaaagaaaa
    16441 tggaaatctt tgggcttttg ctttattcaa cgtgattgcc gcaattgttt tagccattac
    16501 attagccgta tatcgaacat ggggctgggg aatcacccaa tactcaagtt tgatttatct
    16561 aatattagcc atttatggcg tcgttgttct tttgcaagcg atcctaggac gtgagccaaa
    16621 gaaagcggca gcttaaatga tcgagatgga actggacaca ggaaattttc tgttctagtt
    16681 ctttttttat tgtattacat acatttttgt gtataattat actatcgttt gcaatggata
    16741 gatgattcac attttgattg ttttttcggt aaaagtaaag atttctatat ttatcagtat
    16801 tttttcaaaa accagcagtt atagattgac aaagcctctc gagtatcgta aacttttttt
    16861 gtataaataa ctgaatacga gatgcctcaa tcaatgaggt agaggtcgcg agaatcatta
    16921 accctgtgga gtgaagcaaa catggagaag caggaatagg gaggatcgcc gaaatgtgca
    16981 cttttttgct tggagtgtat gttgggacgc aagttaagag cttgcggact gtcttagcag
    17041 tgatgctaag ttgcgctatg ttttgtgaga ggtttatcac cttatggaat tttagcggcc
    17101 tttttatatc tataaatcgg tcgcttttta ttttttcaga taaagcttta ccgcatttca
    17161 tctagaccgc tcgtgtatca tataatcaga aagggagaaa aaaatgaaaa aaaatatggt
    17221 ttcagttgcc gtactatttg cttttgtctt ctcactattc agtgtgggat ctctggcaaa
    17281 tgcagatgaa aaaacaccga gcgatgagtt tcgcgtaggt atggaggctg ggtatgcacc
    17341 cttcaactgg tctcaacaaa cagatgagaa taatgccttg ccaattcaag gccaaaatag
    17401 ttacgccggc ggttatgatg ttcaaatcgc taaaaaagta gcagatggtt tagggaaaaa
    17461 attagtcatc gtccaaacga aatgggatgg tctggctcct gcccttcaat caggcaagat
    17521 cgacgcgatc atcgccggaa tgagccctac tgctgaacga cgaaaagaaa ttgattttac
    17581 cgatccttat tacgaatcac aattagtagt agtagttcaa aaagatagca aatatgcaaa
    17641 agcaaaaagt ttagcagatc tttctggagc aaagatcact gctcagttaa atacattcca
    17701 ctatactgtg atcgatcaga taccagatgt gcagaaacaa caagcaatgg ataatttctc
    17761 agcaatgaga acagctttag cttctggaat gatcgatggt tatgtcagcg aacgtccgga
    17821 aggtgtaacg gcaactagtg tgaacaaaga cttggaaatg cttgaattca gcggtgcaga
    17881 tggcttccag acaaacccgg aagatgttca gatttctgtt ggtatgcgta aaggcgaccc
    17941 ggatatccaa aaagtaaacc agattctttc tggcatctct aacgatgaac gaaccaaaat
    18001 tatggaccaa gcagtcaaag atcagcctgc tgcaactgat agtgaagaaa aacctggcat
    18061 catttctgat ttcactacga tttggaaaca atacggcaat atgttcttac gaggtgctgg
    18121 acttaccttg ttcatcgcat tgatcgggac agttgtcggt actacacttg gattactgat
    18181 tggtgtcttc cgttcggtac ctgaatcagg aaatgcactg actcgcttct tccaaaaaat
    18241 cggaaatgca cttctttcta tctatgtcga agtattccgc ggaacaccga tgatggtaca
    18301 agcaatggtc atcttctacg gtctggcact cgcttttggc atttcattga accgaacgat
    18361 tgccgctttg tttattgtct ctctcaatac tggagcttac atgacagaga tcgttcgtgg
    18421 ggggattttt gcagtagatg aagggcagtt cgaagctgcg caagccattg gtatgaccca
    18481 tggccaaacg atgagaaaag ttgttattcc acaagtttta cggaatatct tgccagcaac
    18541 aggaaatgag tttgttatca atatcaaaga tacagcagtt ttaagtgtaa tcggcgtagc
    18601 tgatttgttc ttccaaggaa actctgcttc cggtgcgaat ttccaattct tccaaacatt
    18661 tacgatcgtc gggatcatct acttagtcat gacctttacg atcaccagaa tcctgcgggc
    18721 ggtagaaaag aaaatcgatg gtccttcagc ttataccaag cttgaagaaa tcgacaattc
    18781 aaatttgcag gaataaggag gaagaatcat gaatgaaaaa attattgaaa tcaaacatct
    18841 aagaaaaagc ttcggtgaaa atgaagtatt gaaagatatc agcgtaacag tcaataaagg
    18901 tgaagttgtg acgatcatcg gttcttccgg ttctggtaaa tctactttct tgcgctgtat
    18961 caatttactg gaaaaaccaa caggcggcga gatcatttat aatggagaaa atgtattagc
    19021 tcctaaatat aatttaccta aatatcgaac gaatctaggt atggttttcc aatccttcaa
    19081 cctgttcaac aatatgaatg tcttggaaaa ctgtatgtct ggtcaaacaa ctgtcctgaa
    19141 acgagataaa gaaactgcca aaaaagtagc aatcgaaaat ctggaaaaag ttggtatgga
    19201 tcgttatatc gaagccaaac cagcacaatt atctggtgga cagaaacagc gtgtcgcaat
    19261 tgctcgggca ttatcaatga atccagatgt attgctattc gacgaaccta cttcggcgtt
    19321 agaccctgaa atggttggcg aagtgcttaa tacgatcaaa gacttagcac atactggctt
    19381 gacaatgctt atcgtgacgc atgagatgga atttgcaaaa gatgtatctg accgtgtcat
    19441 ttttatggat aaaggtgtga ttgcagaaga aggtacacca gaagatatct ttgtccatcc
    19501 gaaagaagaa cgtacaaaag aattcctttc acgtatttta aatgcttagg atcaatagct
    19561 gattgggaca aagagctgcg gtgtttaccg cagttctttt ttatgaatta ctcataaaag
    19621 gtaaatttct gaaaattttg gaatcttagt tgtgtttttc agaaaactct cttaatatgg
    19681 aagtaattaa acttttttag ttaggatgat ttaaaaatga atgaagagca agaactgcag
    19741 cgtggactga aaaatagaca tgtccaactg atttctattg gcggagcgat tggaacgggg
    19801 ttgtttttag gttccggaaa gtctatccag ctagctggcc cttcgatatt gctcgcctat
    19861 cttattactg gatgcatctg ttttttcatt atgcgtgcgt taggcgaact tctgttatca
    19921 aacaccaata accattcttt tctggatttt gttgcagaat atcttggcaa aaaagcggct
    19981 ttcatcactg gctggacgta ttggttctgc tgggtatcga ttgcaatggc cgatttaaca
    20041 gccatcggaa tgtatgtacg ctactgggcg cctggtgttc ctcagtggct tcctgaactg
    20101 attgcattag gtcttcttct ttgcttgaat atggtggctg tctcattgtt tggtgagtta
    20161 gagttttggt ttgcattgat caaagttgta gcgatcattg cctttatcct cgtaggagct
    20221 tatatgatcc tcacccacta tacgacagat attggcacag ccagtatcac gaatctatgg
    20281 tctcatggcg gattctttcc aactggtgca aaaggattta tcttagcttt ccaaatggta
    20341 acctttgctt ttgctgggat agaattagtt ggattagtcg ctggagaaac agaaaatcct
    20401 gaaaaagtgt tgccggaagc tatcaataat attcctatca ggatcatctt attttactta
    20461 ggatctcttt ttgtcattat ggcaatctat ccatggaaca gcttgaatcc agataacagt
    20521 ccatttgtag aagtattttc ggaaattggg atcacgattg ctgcttcctt gatcaattta
    20581 gtcgtattaa gtgcagcagc ttctgcctgc aacagtgcaa tatatagtac aggacgtatg
    20641 cttcgttctt tagcgcaaga aggcagtgcc cctaaaaaat tcaaaaagtt aacaacacat
    20701 catgtgccag ggaatgcttt gacattttct actatcgtca ttttcatttc tgttatcttg
    20761 aattacgtga tgcctagtga agtattcaca ctagtaagca gtattgccac tacttgtttc
    20821 ttgtttatct ggagtatgct tgtctacacg cacatgaaat atcgtaaaag tatcctcggc
    20881 aaaaaagctc attcatttaa aatgccgttg tacccattta gtaattatct ggtatttctt
    20941 tacatggcct ttgtgtgtgt cgtcttgttc ctaggaaaag atacgagaat cgctttatta
    21001 ctaacacctg tgtggttctt gcttctatta ctaatctatc atatgaaata tgaacgtaag
    21061 aagtaagaaa gtagttgtga cagaagtggt cagcttcgag aaataagaag gaatttacga
    21121 aaattgcttc ttaaattttt gtgaaatttc ggcttatttc ttggagataa aagcttctgt
    21181 cccagcctct tttctatttc cccggaaaca accgctgata ataggcatct gcttttttat
    21241 aggaaaaaat ccaaatacca gatacaaaaa aacgtccgaa taacccgatc aacggtacaa
    21301 aaaagacacc ggcaatcaag cataagttaa tcatcatgga gataagactt cgacggtcct
    21361 ttgacaaaac tctcacgaca tgggaatctg gtttatttgc tttcatgatt tgcctcatca
    21421 agataccaaa agaaaaactc cacatcgtgt atactaagac ataagccatc tcaggccaaa
    21481 aagcttcagg gttacgcccg acccaactag taacagaagg acataaagtc agccaaaaga
    21541 gcaaaaagat attcgaccag accactcggt aattaattcg ttctgccatc tgaaatatac
    21601 gatgatggtt cacccagatc accgcaacaa atataaagct gatggtataa gctaataatg
    21661 tatttcggat gacccataaa gaatgaaagc tagatgtatt agggatcttg atctctaata
    21721 caataactct caaaacaatg gctaccactc catcagtaaa tgcttctaat ctcgactttg
    21781 tcacgatgat tcctcctcag atgattcagc gcttttattt tatcacttac gttattaaat
    21841 aaccatcgtt ttatataggg aattttctga aaattgttgc atatttttcg aaattttgtt
    21901 acttttaaaa aa
//
LOCUS       DACEEZ010000044        21573 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20313_2017
            SAMN12639966-rid9879223.denovo.044, whole genome shotgun sequence.
ACCESSION   DACEEZ010000044 DACEEZ010000000
VERSION     DACEEZ010000044.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639966
            Sequence Read Archive: SRR10023158
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 21573)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21573)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741683.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741683.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:44:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,016
            CDSs (total)                      :: 2,962
            Genes (coding)                    :: 2,889
            CDSs (with protein)               :: 2,889
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 36 of 73
            Pseudo Genes (incomplete)         :: 29 of 73
            Pseudo Genes (internal stop)      :: 18 of 73
            Pseudo Genes (multiple problems)  :: 8 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21573
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639966-rid9879223.denovo.044"
                     /isolate="E20313_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            <1..481
                     /locus_tag="IWO89_001654"
     CDS             <1..481
                     /locus_tag="IWO89_001654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HAQ0456402.1"
                     /translation="KNAWELKGQHRYWRPSFRDHLTEAEIVDRLLSYDDSLKRGYEVY
                     QDFLSVIRRQDVPEFVALLKEDYKELPEHYQPVFTTFKKYQTEIKRALRVPYSNGPIE
                     CLNNHIKVLKRIAYGYRNFQNYRERIFLYRGKYFKKTKNTTQLTKARTTTRLDKIAV"
     gene            complement(722..2338)
                     /locus_tag="IWO89_001655"
     CDS             complement(722..2338)
                     /locus_tag="IWO89_001655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002406986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HAQ0456403.1"
                     /translation="MKKIPITILTGYLGSGKTILLNHILYGDHGKEIAVIVNDIGEVN
                     VDGVLVDKKGFKRTGEKLVSMPNGCICCTLREDLIVELDQLVQFSDLNQIVIEASGIS
                     KPIPIIQAIILAKNPLDIDLTQRLEIGAIIIVVDVKRFWDNFETGEKIQERKAEGNVV
                     EEEQDIRGLLIDQFECCNILLLNNSDLVSTKTIYKINGLIAKLQSNAKIIRTIKGEVD
                     LSKILNTNLFNIDETSYSVGWFKELELGYQNHIPETEEYGISSFIYRNRLLLDAQKFA
                     HLTDKNFPGNITRAKGFLWFAQHSDFSTIFETSGSYKSIESLNYWVASLPKIECQMII
                     QKNPELKEAWHPLFGERINKLVFIGMKMDQQCIKEQLEGCLTDSLNADNFRKISPFPW
                     KKSNMFSQISLMKFLRKAGVKIGDVIIIGILIITSFIPLFVFSIEKENETTEDAIKYA
                     IVRIDGKEMDRFDLDKINHKLVNYHPAKGQYNIVEIKDGRIRVKEDNSPDQIAVRTGW
                     ISKPGQTSICLPHKLVISIEKKESTDYYIY"
     gene            2456..2674
                     /locus_tag="IWO89_001656"
     CDS             2456..2674
                     /locus_tag="IWO89_001656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAQ0456404.1"
                     /translation="MSQYRHLTILERERIFLLHEEGFSISIIGNDIQHSPFTILHELR
                     RNKKKTIHLPKRKKIMLIEEKNADVITF"
     gene            2869..3330
                     /locus_tag="IWO89_001657"
     CDS             2869..3330
                     /locus_tag="IWO89_001657"
                     /inference="COORDINATES: protein motif:HMM:NF033563.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HAQ0456405.1"
                     /translation="MHHRGKTRHTKNHVESRGKICLTHSIHGRPKSADDRSDFGHWKA
                     DIVAGKTGGTCLVTLTDRKSCFLLAGKKVPRKKAAYVSEKMIELLSSIPNEAVKTITP
                     DRGKEFSNHFAVTEALNNMPFYFPDPHALGNNEQIKIRIDSFENTCQNRKR"
     gene            complement(3523..3762)
                     /locus_tag="IWO89_001658"
     CDS             complement(3523..3762)
                     /locus_tag="IWO89_001658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0456406.1"
                     /translation="MVRKVLLSMDSKNIDAYRRYRFAKQMRLNGSTLADIAATLHVSI
                     DTVHRVLKRDPDCCFIKVNTGRSRRLAKRHNSLAN"
     gene            complement(3786..5309)
                     /locus_tag="IWO89_001659"
     CDS             complement(3786..5309)
                     /locus_tag="IWO89_001659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein
                     AdcA"
                     /protein_id="HAQ0456407.1"
                     /translation="MKKISVGLIGIAALGLLGACSSTNDAKVSNDKDGKLEIVTTFYP
                     MYDFTKNIVGDEANVDLMVPAGSEPHDYEPSAKDMAKAHDADVFVYHNENMESWVPKA
                     KESWKKAGPNVVEGTKDMILLPGSEEEDHDHGEEDHHHELDPHTWVSPKMAIKEVSNI
                     KDQLVKLYPKKAKVFETNAEKYLTKLKRLDADYTTSLKEAKQKSFVTQHAAFGYLALD
                     YGLKQVPIAGLSPEEEPSSGRLAELKEYVKKNKINYIYFEKNANDKIAKTLANEAGIK
                     LEVLNPLESLTKEQMDNGEDYVSVMEDNLKALEKTTMVAGEEVVPEKEAKDEKTVANG
                     YFKDADVKDRELSDYTGEWQSVYPLLKDGILDEVFDYKAKINKDMTAAEYKDYYTTGY
                     KTDIDTINIKDNTIDFVVNGEHHQYTYKYVGYKILNYEKGNRGVRFNFETDDAGAGRF
                     KYIQFSDHGIAPSKAEHFHIFFGGESQEKLYNELHNWPTFYPASLSKHEIAQEMMAH"
     gene            complement(5327..5449)
                     /locus_tag="IWO89_001660"
     CDS             complement(5327..5449)
                     /locus_tag="IWO89_001660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative metal homeostasis protein"
                     /protein_id="HAQ0456408.1"
                     /translation="MEKTDISSAYRRLKSPNIKTRKRALKIIKEHKRNKMKKLA"
     gene            complement(5479..6462)
                     /locus_tag="IWO89_001661"
     CDS             complement(5479..6462)
                     /locus_tag="IWO89_001661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HAQ0456409.1"
                     /translation="MGIPVTIVSGFLGAGKTTLINQALQVSPYPREEIIIIENEFGEV
                     GIDHKLLVHSEERVLQLNNGCMCCSLRSDLLAILISLLETIEEHQQPISQIIIETTGI
                     ADPQPIIQTLLTAPQLRGRVYIDSLLTVIDSDSFELIEREPQALKQLVMADRIFVSRK
                     QNLNLSALKKLQREIKSINPLSDIRMFTHSETIQKKDFYNLKKFNHPLVFESDSSENH
                     YNGKYYHQHHQVHEFKSILLTSDQDIKKDFLLQWIDWLILTNQGSLYRFKGLIALQEQ
                     DFIVAMQEVNEQVNFQYTTRQKDQTTIILIGKMLDEERIKQSFDKLLAESK"
     gene            complement(6486..6635)
                     /gene="rpmG"
                     /locus_tag="IWO89_001662"
     CDS             complement(6486..6635)
                     /gene="rpmG"
                     /locus_tag="IWO89_001662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003550836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HAQ0456410.1"
                     /translation="MRQNIILECVETGERLYLTSKNKRNNPERLELKKYSPKLRRRAI
                     FKEVK"
     gene            complement(6656..7033)
                     /locus_tag="IWO89_001663"
     CDS             complement(6656..7033)
                     /locus_tag="IWO89_001663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0456411.1"
                     /translation="MEIIYPPLVEEGLKYYLETTQQSLDKCTFYRSMVERGIITETGL
                     PTQQAIENGLVKDYYEDQGLSFDDFLRIYPIFEEYDEELFQCIDGYWEIPVDMKENLV
                     SQLESGELTFEDAQQIQAYLEDR"
     gene            complement(7065..7244)
                     /gene="rpmF"
                     /locus_tag="IWO89_001664"
     CDS             complement(7065..7244)
                     /gene="rpmF"
                     /locus_tag="IWO89_001664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018345355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HAQ0456412.1"
                     /translation="MAVPARKTSKAKKRLRRTHQTVVKPEISFDEANGDYRRSHHVSL
                     KGYYNGKKVIKGASK"
     gene            complement(7259..7528)
                     /gene="rpsN"
                     /locus_tag="IWO89_001665"
     CDS             complement(7259..7528)
                     /gene="rpsN"
                     /locus_tag="IWO89_001665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S14"
                     /protein_id="HAQ0456413.1"
                     /translation="MAKKSKIAKAKKQMAMIEKYADKRQELKAAGDRTALAKLPRDSN
                     PNRLRLRDQTDGRPRGYMRKFGMSRIKFRELAHQGLIPGVKKASW"
     gene            complement(8051..9823)
                     /locus_tag="IWO89_001666"
     CDS             complement(8051..9823)
                     /locus_tag="IWO89_001666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002841847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ0456414.1"
                     /translation="MRMQMIGGLTMKSFYKNKFALSDQGAEALTKASISSFFVYCINM
                     VPAFIIMMLIDELVLENTKPRWLYFAVSFVTLLFMYWLLDREYENLYNSTYKESAHLR
                     VQIADDLSNLPLSYFSKHNLSDLSQTIMSDVEGIEHAMSHAIPKAGGMALFFPFISVM
                     LLVGNVKMGLAVILPTLFSFVLILLSKKSQTKANTKYYDTLRENSEEFQETIELQQEI
                     NSFNLSKKVQDRLFKKMEESERIHLKVELSTFSVMALSSIFSYVSLAVVILVGVHLLL
                     TGEVTILYVVGYLLAAIKIKDSFDSMKEAVLEIFYLAPKIQRIRAMKETSIQEGSDSP
                     LKSFDIELRDVSFSYDNNTPILDHISFTAKQGEVTALVGASGSGKTSILKLVSRLYDY
                     DEGCILIDGYDIKRVSPASLFSKIAIVFQEVTLFNTSILENIRIGNSQASDEEVKKAA
                     RLANCEDFIEKLPDGYHTLVGENGSSLSGGERQRLSIARAFLKNAPILILDEITASLD
                     AENEKRIQESLNRLIQDKTVLIISHRLKSIEKVNKIVVMDQGKLVDQGTHNELYRRSE
                     IYKNLIKKTKLSEKFVYEKEAQSR"
     gene            complement(9777..11531)
                     /locus_tag="IWO89_001667"
     CDS             complement(9777..11531)
                     /locus_tag="IWO89_001667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016180774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ0456415.1"
                     /translation="MKVYKKLFSYVPKMKYYGWLATLFSDFSVVLTVYGYYSIYKFLQ
                     SLIIVGDEYLAKSYAVQTVAWLTLGALFYIFSGLFSHILGFRLETNLRKKGISGLTEA
                     SFRFFDLNSSGYIRKTIDDNAAKTHMAIAHLIPDSAQAIVTPVLSVALGFFINFKIGL
                     VLIAMLVIGVFLLKKMTENTNFIQKYQESLDVLSSETVEYIRGIQVIKIFGAKVTSFK
                     ALNKAINDYAKFAYKYSLSGKTPFVLFQWLFFGIVCILIVPITFFITSLTTPKILAVD
                     LLMLFFLSGIIFVSFMRIMYVSMHLFEARYAVETLDSLYLEMKQDQLKYGNETQIKNC
                     DIAFENVSFSYKEECVLENFNLVLEQKRIYALVGKSGSGKSTIAKLISGFYNVDKGQI
                     KIGGKPLNHYSKDTIINTIAFVFQDTKLFNQSIYDNVSIANPQAPASKVFDALRLAGC
                     ESILEKFPEREHTLIGSKGVYLSGGEKQRIAIARAILKDAKIVIMDEASASIDPDNEY
                     ELQKAFQHLMKEKTVIMIAHRLSSIKSVDEILVLSEGKIVERGNHNELMAKHQVYKQL
                     VTNYENANDWRLDNEKFL"
     gene            complement(11641..12210)
                     /locus_tag="IWO89_001668"
     CDS             complement(11641..12210)
                     /locus_tag="IWO89_001668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MptD family putative ECF transporter S
                     component"
                     /protein_id="HAQ0456416.1"
                     /translation="MKTKDYIFIGLATVISLVIYFFSIMVSSIGGAFGHSISPGIFGL
                     LSGIIFVYISYNYPRKGIFTIYTIVLLLFFTLMGGAYLPWFISSISMAILADIILSVF
                     GYDRAIPQVASWALMQLGSAAGQWIPIWFFTDRFRQDWIDKGQSAATMDAMIHYAVGI
                     WGIISVLVVASLSMIGVLIGRKVLKKYKK"
     gene            complement(12228..13652)
                     /locus_tag="IWO89_001669"
     CDS             complement(12228..13652)
                     /locus_tag="IWO89_001669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter
                     ATP-binding protein"
                     /protein_id="HAQ0456417.1"
                     /translation="MKELFEFNNVSFSYKDVKEQALVDLNFSIKKGSLTLITGSSGSG
                     KSTLLKLMNGLIPRLYDGKLVGEILFENNSLQSLTQESISKNIGYVSQDPRGQFFTTN
                     TTSELVFSMENFGFTKEHMDKRINELAELLDLSNILDKNIFSLSSGERQKISIGCSLS
                     LNSKLIILDEPSSNLDFKATKKLADLLCDLKNKGFTIVIAEHRFYYLKELIDQVLFVK
                     NRTVKTTTIQLLKTENNNALRKFDIFNSSIEVPYIEKVSADVLKVEEITYENILKDIN
                     FDVKVGDVIGLVGKNGVGKTTLLRILMKIIRPTKGKIIENKKNLSSPFLVMQEMDYQF
                     FTESVRSELALGNEIVSKDQQEKILKKMELYKMKDQLPFDLSGGEKQRLLVSIASLSK
                     TNLFLFDEPTSGLDYINMERIADLIKDLKEKGAVVIATHDPEFLYKTCNRIIYLKNGG
                     IKKDIKLRVEDSTLIEQIFNDITK"
     gene            complement(13654..14322)
                     /locus_tag="IWO89_001670"
     CDS             complement(13654..14322)
                     /locus_tag="IWO89_001670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     protein EcfT"
                     /protein_id="HAQ0456418.1"
                     /translation="MSKINPLTLITLNIFFPVIIFLGKGNIFQVGCFIIATVLVLMFR
                     KIKTCIYFILIYSVLFLVMKIIDAIKIEFFTIFFATSLYIVFRMLPVVMISWLLVSVY
                     TSNELLSSLEKIHLPKKIMLSITVVIRFFPTYRSEIRMIKESLKMRNIRLSFFQPLQY
                     LEFLLVPVLIRATMIAEEMTANAITKGIESPVKRSSYYKTNMSLLDYIILFSVTIFFL
                     SILI"
     gene            complement(14452..15036)
                     /locus_tag="IWO89_001671"
     CDS             complement(14452..15036)
                     /locus_tag="IWO89_001671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HAQ0456419.1"
                     /translation="MESGLSHFEKEGFQQTKIRQLCLTAGVTTGAFYKHFTSKEALFE
                     EIIQPHINALINMYNEEFTKYLLNLENIDIEKSWRENFNELDPFINYIFENKRIFDLI
                     LFQSAGTKYQDIVEKITSFVTKKTVCCLDKLKTIGMINQNLVFNEEEIHFYIYSFYAT
                     FYDILKHSYSKEKTMLLTRRLYQFSVPGWIELLS"
     gene            complement(15367..15600)
                     /locus_tag="IWO89_001672"
     CDS             complement(15367..15600)
                     /locus_tag="IWO89_001672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002382893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-dependent repressor"
                     /protein_id="HAQ0456420.1"
                     /translation="MVQIKTTTILIYLLAIQELSKRKKKLKNNDLAKVLSVSPASVSE
                     MLAKLRYEDYLDIGFQLTDKGKKFIDDYKKGFK"
     gene            complement(15615..16565)
                     /locus_tag="IWO89_001673"
     CDS             complement(15615..16565)
                     /locus_tag="IWO89_001673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter substrate-binding protein"
                     /protein_id="HAQ0456421.1"
                     /translation="MMRKWKAVLGSLGILIALFIFGACSINSKDKDKVASNEKLKVVV
                     TNSILADITENIAKDKIDLHSIVPIGKDPHEYEPLPEDVQKTSKADLIFYNGVNLETG
                     GNAWFTKLVKNANKEENKDYFAASDGIDVIYLEGQSEKGKEDPHAWLNLENGIIYAKN
                     IEKQLAEKDPDNKKFYKENLDKYIEKLDSLDKEAKSKFASIPNDKKMIVTSEGCFKYF
                     SKAYNVPSAYIWEINTEEEGTPDQIKHLVEKLRTTKVPSLFVESSVDDRPMKTVSKDT
                     NIPIYSTIFTDSIAEKGQDGDSYYAMMKWNLDKIAEGLSK"
     gene            complement(16562..17404)
                     /locus_tag="IWO89_001674"
     CDS             complement(16562..17404)
                     /locus_tag="IWO89_001674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HAQ0456422.1"
                     /translation="MLQNFINGLYDFHFLQNALITSVVIGIVSGTVGCFIILRGMSLM
                     GDAISHAVLPGVAISYILGINFFIGAIVFGLLASIIITFIKSNSVIKGDTAIGITFSS
                     FLALGVILIGVANSSTDLFHILFGNILAVQDIDKWITIGVSVLVLVIVVLFFKELLIT
                     SFDPLMAKAIGMNVSFYHYLLMVLLTLVAVTAMQSVGTILIVAMLITPAATAYLYANS
                     LKTMIFISATVGASSSLLGLFIGYSFNIAAGSSIVLTAAFLFVISFLISPKQKFLRKK
                     ERSL"
     gene            complement(17406..18125)
                     /locus_tag="IWO89_001675"
     CDS             complement(17406..18125)
                     /locus_tag="IWO89_001675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HAQ0456423.1"
                     /translation="MLEVKKLNVCYNDFLALNEISLKIQEGALTGIIGPNGAGKSTLL
                     KAMLNIIPHQGEVSINNEDINKKLVKIAYVEQKADIDFTFPIKVKECVSMGTYAGMKV
                     FQRIKNAEWQRVSKALEKVDMGKYSNHQIGELSGGQFQRVLLARCLAQNADFIFLDEP
                     FVGIDLVSERIIMDTLKKLKHQGKTVLIVHHDLSKVKKYFDNIILLNRNLIAHGSVES
                     VFNEENLKKAYGDTIFIGEGE"
     gene            18817..18975
                     /locus_tag="IWO89_001676"
     CDS             18817..18975
                     /locus_tag="IWO89_001676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAQ0456424.1"
                     /translation="MNKKQTCAFLGIDNNTLDKWIRIYNFPVIRVGGVVRFDVEDIKD
                     FMNTFKIT"
     gene            19047..20273
                     /locus_tag="IWO89_001677"
     CDS             19047..20273
                     /locus_tag="IWO89_001677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HAQ0456425.1"
                     /translation="MPTKLSNGKYKTNLRYPKRFKEITGIASEKYQKTFPNRQLAIKA
                     ENDMKKKIEKVLREENANSLELKGKITFKKFYESKWLPRYELGQTIRSNRPPSDITIS
                     NTKDIFRLHILPMFGEYAMNYLNINTEIISDELTKKSKEYANIKIIKGYVRSMFDIAE
                     ILNYIEFNRTTKIIQSITAPKKNALEEKRIQEGKQALSSKELTNWIEAVNDDFNNHLL
                     TFHDYTLFMITLYLGDRKSETYALQWKYIDFEKQTVRLKHTLDKYQRKKFTKGRKDTV
                     IQVPEVVMTLLSEWKSVQADQLLKLKIKQTLDQYLFTYTKPSGEVNCPVHADYLNYRI
                     NSIKRRHPDLVHLSPHKLRHTYATIARQGGADMNQISNALTHSDISTTKIYVNTPDIV
                     DKAVFEAFQRGLNKCD"
     gene            complement(20361..20753)
                     /gene="rpsI"
                     /locus_tag="IWO89_001678"
     CDS             complement(20361..20753)
                     /gene="rpsI"
                     /locus_tag="IWO89_001678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002942223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HAQ0456426.1"
                     /translation="MAQVQYIGTGRRKNAVARVRLVPGTGKITVNKKDVEEYIPHADL
                     REVINQPFAVTETKGAYDVFVNVNGGGYAGQSGAIRHGIARALLEVDPDFRLALKRAG
                     LLTRDARMVERKKPGLKKARKASQFSKR"
     gene            complement(20769..21212)
                     /gene="rplM"
                     /locus_tag="IWO89_001679"
     CDS             complement(20769..21212)
                     /gene="rplM"
                     /locus_tag="IWO89_001679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HAQ0456427.1"
                     /translation="MRTTYMAKPGEVERKWYVVDATDVPLGRLSTVVASVLRGKNKPT
                     FTPHLDTGDFVIVINADKVKLTGKKATDKIYYRHSNYPGGLKSITAGELRAKNSRRLI
                     ETSVKGMLPKNTLGRKQFTKLNVYGGAEHPHAAQQPEVLDITNLI"
BASE COUNT         7047 a         4016 c         3103 g         7407 t
ORIGIN      
        1 aaagaatgcg tgggaattga aaggtcagca tcgctactgg cgtccgtcat ttcgagatca
       61 tctgacagaa gcagagatag tcgatcggct attatcttat gatgattcct taaagcgagg
      121 ctacgaagtc taccaggatt ttctttccgt tatcagaaga caagatgttc ctgaattcgt
      181 cgcgctccta aaagaagact acaaggagtt gccagagcac taccaaccag tgttcacgac
      241 cttcaagaaa taccaaacag aaatcaaacg agcgttgcgc gttccctatt ccaacggccc
      301 tatagaatgt ttgaataacc atatcaaagt actaaaacga attgcctatg gctatagaaa
      361 ctttcaaaac taccgagaac gaatcttttt atatcgagga aaatatttta agaaaactaa
      421 gaatactacc caactgacta aagccagaac aacaacacgg ctagacaaga tagctgtcta
      481 gccgtgtgag tatctcgatt attctattga atttcttcac caacactcat tgacgaagag
      541 ccaaaataag taatctaacc gactgttaac taaaccattg caggtcccgt taaagatact
      601 tgtgcctgtt gtcaccgctt ctttttcttg tccacaagcg cttaacacac ttaataaagc
      661 ccctgctatt attaataata atatcttttt cttattaaaa acctcctcat ctatttccag
      721 attagtaaat gtagtaatct gtgctttctt ttttctcaat actaataact aacttatggg
      781 gtaaacaaat actcgtttga ccgggttttg agatccatcc agttctaacc gcgatttgat
      841 caggactatt atcttcttta actcgaattc ttccatcttt aatctcaaca atattatatt
      901 gtcccttcgc aggatgataa ttcacgagtt tatgattgat cttatccaaa tcgaagcgat
      961 ccatttcctt gccatcaatt cgaacgatgg catatttgat agcatcttca gttgtttcat
     1021 tctccttttc tatagagaat acaaataagg gaataaaaga ggtaataatc aaaatgccaa
     1081 taattatgac atcgcctatc ttgacaccag cttttcttaa aaatttcatt aacgatatct
     1141 ggctaaacat attagacttt ttccatggaa atggtgaaat ttttcggaaa ttatctgcat
     1201 taagagaatc agttaaacaa ccctcaagtt gttccttaat acattgttga tccatcttca
     1261 taccaataaa aactagttta ttaatgcgtt caccaaacaa cggatgccag gcttccttca
     1321 attcgggatt tttttgtata atcatttgac actcaatttt aggtaaacta gctacccaat
     1381 aatttaatga ttcaatactt ttatagctac ccgatgtttc aaagatcgtc gaaaaatcac
     1441 tatgttgggc aaaccaaaga aacccttttg ctcttgtaat attgccagga aaatttttat
     1501 ccgtaagatg ggcaaatttt tgtgcgtcaa ggagtaaccg atttcgatag ataaaacttg
     1561 agataccata ttcttctgtt tcaggaatat gattttgata gccaagctct aactctttga
     1621 accagcccac tgaataggaa gtctcatcta tattaaaaag atttgtattt agaattttcg
     1681 ataagtctac ttcaccttta attgtcctaa ttattttggc attggactgt aacttagcaa
     1741 ttagaccgtt aattttgtaa atcgtcttgg ttgacactaa gtcactatta tttaaaagta
     1801 atatgttaca acactcgaat tgatcaatta acaagccacg aatatcctgt tcttcttcaa
     1861 ctacattacc ttcagctttt ctttcctgaa tcttttcacc cgtttcgaaa ttatcccaaa
     1921 aacgttttac atccactact ataataatgg caccaatctc taatcgttgg gtcaaatcaa
     1981 tgtctaaagg gtttttagct aatataattg cttgaataat tggaattggt ttgctgattc
     2041 ctgaagcttc aataacaatt tgattcaagt cagaaaattg cactaattga tctagttcaa
     2101 caatcaaatc ttcgcgtaat gtacaacaaa tgcatccatt cggcatcgaa acaagttttt
     2161 ctccagttct tttaaaacct ttcttgtcga ctaatacccc atcaacattt acttccccta
     2221 tgtcatttac aatgacagca atttcctttc cgtgatcacc gtacagaata tgatttagaa
     2281 gtatcgtttt tcctgaacca agatagccag ttaagattgt aataggtatt tttttcatag
     2341 ggaacctctt tctatacctt gaatttacac tttaagtgca acacataaaa atgagtcata
     2401 atcgatatcc aactagtata agtttgtcga gaacactcct agaaggaaaa taattatgag
     2461 ccagtatcgt catcttacta tactcgaacg agaaagaatc tttctgcttc acgaagaagg
     2521 attttctatt agtataattg gcaacgacat tcagcatagc ccgtttacta ttttacatga
     2581 actgagaaga aacaagaaaa aaactattca ccttccaaag cggaaaaaaa ttatgcttat
     2641 cgaagaaaaa aatgcggacg ttatcacatt ctaaatgatc cgtgagtttg gtcaattgtt
     2701 cgtcatttat ttgtcgattt tcagtggtcg ccagaggaaa tctcgaatcg tttgaagcat
     2761 gaacatatgg acttttcaat tagttttatc acgatctaac gtgcaatttg caatgatctt
     2821 ttcgatacag aacccctctc acacggcaat aaaggcctga tccgttcatt gcaccatcga
     2881 ggaaaaacaa gacatacaaa aaatcatgtc gagtctcgtg gaaagatttg ccttactcat
     2941 tcgatccatg gccgtccaaa atccgcagat gatagaagtg attttggtca ttggaaagcc
     3001 gatattgtag ctggcaaaac tggtggtact tgtttagtga ctttgacaga cagaaaatca
     3061 tgcttccttt tagcaggaaa gaaggtcccc agaaagaaag ccgcttatgt gagcgagaaa
     3121 atgattgaac ttttaagcag tattccaaat gaggcggtca aaacgatcac tccagataga
     3181 ggaaaagaat tctccaatca tttcgcagtt acagaagcat tgaataatat gcccttttat
     3241 tttccagatc ctcatgccct tggcaacaat gaacaaatta aaatacgaat agactcattc
     3301 gagaatactt gccaaaatcg aaagagatag ctacggtaga taactcaacg ataaatcaga
     3361 tgattgaaaa actgaataac agacctagaa agtgtttaga ttggagaaca ccttatgaag
     3421 tcttctttga taaagtgttg cgcttgattt gacaattcaa gacataataa aaagcagatc
     3481 aaatccaaat aagggaaaaa tgatctactc tggtattatc aattaattgg ccaagctatt
     3541 atgccgtttt gctaaacgtc tacttcttcc agtattgacc ttgataaaac aacaatcagg
     3601 atcccgtttt aatactctat gaactgtgtc aatactaaca tgtaaagttg cagcaatatc
     3661 agctaatgta ctcccattta atctcatttg ctttgcaaat cgataacgcc gataagcatc
     3721 aatattcttg gaatccatag acaataatac ttttcttacc atgtatttcc tccaaaaatt
     3781 gaattttaat gagccatcat ttcttgagca atttcatgtt tagacaatga agctggatag
     3841 aaagtaggcc aattatgcaa ttcattatag agtttttctt gactttctcc accaaagaaa
     3901 atatggaaat gctctgcctt acttggtgca attccatgat cactaaattg aatatattta
     3961 aaacgaccag cacctgcatc atcagtttca aaattaaaac gaacaccacg gtttcctttt
     4021 tcataattca aaattttgta tccaacatat ttgtatgtgt attgatgatg ttcaccgttg
     4081 actacaaaat caatggtgtt gtctttaatg ttgatagtat caatatctgt cttatagcca
     4141 gtagtataat aatctttgta ctcggcagct gtcatgtctt tattgatttt tgctttgtaa
     4201 tcgaaaactt catccaaaat tccatctttt aacaacggat aaacagattg ccattcgcct
     4261 gtataatcac tcaattcgcg atccttcaca tcggcatctt taaagtaccc attagcaact
     4321 gtcttttcat ccttcgcttc tttttctgga actacctctt caccagcaac catagttgtt
     4381 ttttctaacg cttttagatt gtcttccatt actgaaacat aatcttcacc attatccatt
     4441 tgttcttttg taagactttc aagaggattc agtacttcca attttatccc agcttcgttg
     4501 gccaaagttt ttgcaatttt atcattagca ttcttttcaa agtaaatata attgatctta
     4561 ttttttttaa catactcttt taattcagct aatcgaccag aagatggctc ttcttcagga
     4621 cttagtccag caataggaac ttgtttcaat ccataatcca aagctaaata tccaaaggct
     4681 gcgtgttgcg tcacaaaact tttttgtttc gcttctttaa gactagtggt atagtcagca
     4741 tccaaccgtt tcagtttagt caaatacttt tcagcatttg tctcaaatac ttttgctttt
     4801 ttcggataca atttaactaa ctgatccttg atatttgaaa cttccttaat tgccattttg
     4861 ggtgaaaccc acgtatgagg atctagttca tgatggtgat cttcttctcc gtgatcatga
     4921 tcttcttctt cactacctgg caataaaatc atgtcttttg ttccttctac tacattaggc
     4981 ccagctttct tccaactttc tttagctttt ggtacccatg attccatgtt ttcgttatga
     5041 tatacaaaaa catcagcatc atgagctttt gccatatcct tagctgaggg ctcatagtca
     5101 tgaggttctg atcccgctgg aaccattaaa tccacgttcg cttcatcgcc gacgatgttt
     5161 ttggtgaaat catacattgg atagaatgtt gttacaattt ctaattttcc atctttatca
     5221 ttcgatactt ttgcatcatt agtgctggag caagctccca ataatcctag agctgctatc
     5281 ccaataagtc ctacactgat tttcttcatt ctttttcctt ccttaattat gctaatttct
     5341 tcattttatt tcgtttatgt tctttgataa tttttaatgc cctttttcta gttttaatat
     5401 tcgggctttt aagtcgtcga tatgcactag atatatccgt tttctccatt agaaattacc
     5461 cccaatcact acttatcatt atttgctttc cgccagtaat ttatcaaaac tctgtttgat
     5521 cctttcttca tccaacattt ttccgattag aataatagtg gtttggtctt tttgtcgagt
     5581 ggtgtattga aaattaacct gttcgtttac ctcttgcatg gcaactataa aatcctgctc
     5641 ttgtaaagca atcaatcctt taaaacgata caggctccct tggttggtta aaatcagcca
     5701 gtcaatccat tggagtaaaa aatctttttt tatgtcttga tctgaagtta agagaattga
     5761 tttaaactca tgtacttgat ggtgttgatg atagtatttg ccattatagt gattttctga
     5821 tgaatcactt tcaaaaacta agggatgatt aaattttttc aaattataaa aatccttttt
     5881 ttgaatcgtt tcactatgtg tgaacattcg aatatcggat aaagggttaa tacttttgat
     5941 ttctctctgt aattttttta gtgcagataa attcaagttt tgttttctag acacaaaaat
     6001 gcgatctgcc atgacaagtt gcttaagggc ttgtggttct ctttcaataa gttcaaaact
     6061 gtcactatca ataacagtca ataaactatc gatatatact cttcctctta gttgaggcgc
     6121 tgttaaaagt gtttgaatga ttggttgggg atctgctata cctgtagttt caatgatgat
     6181 ttgtgaaatt ggttgttgat gttcttctat tgtttccagt aaagaaatca atattgctaa
     6241 taaatcactg cgaagactgc aacacataca tccattattt agttgtaaca cccgttcctc
     6301 gctatgaact aataatttgt gatcaatccc aacttcacca aactcatttt caattataat
     6361 aatttcttcc ctggggtagg gtgagacttg caaggcttga ttgattagcg ttgtttttcc
     6421 agcccctaaa aacccagaaa cgattgtaac tggtatgccc attttgtcac ctttttacca
     6481 cttagctatt tcacttcttt aaaaattgcc cgtctgcgca attttggcga atactttttt
     6541 aattctaacc gttcagggtt attccgttta tttttgctgg tgagatacag acgttctcca
     6601 gtttcgacac attccaaaat aatattttga cgcataaatc ttcctccttc aatacttatc
     6661 gatcttcaag atatgcttga atttgttgtg catcttcaaa agttaactcg ccagattcca
     6721 attgtgaaac taaattttct ttcatatcaa caggtatctc ccaataacca tcgatacatt
     6781 gaaataactc ctcgtcatac tcttcaaaaa ttggatagat tcttaaaaaa tcatcaaatg
     6841 ataagccttg gtcctcatag taatctttga ctaatccatt ttcaattgcc tgttgggtcg
     6901 gtaaacccgt ttcagtaata attcctctct ctaccatcga acgataaaaa gtgcatttat
     6961 ctagcgattg ctgggtcgtt tcaagataat actttaatcc ttcttcaact aaaggtgggt
     7021 aaataatttc cattttgatt ccctcctaaa agttatatat taaactattt tgaagctcct
     7081 ttaattactt tctttccatt atagtaccct tttaaagata catgatggct acgtctataa
     7141 tcaccattcg cttcgtcaaa tgaaatttct ggttttacta cagtttgatg agttcttctt
     7201 aaccgtttct tcgccttaga tgtttttctt gctggtactg ccatgatatt tcctcctttt
     7261 accaacttgc tttttttact ccaggaatca acccttgatg ggcaagttct ctaaacttaa
     7321 tacgggacat accgaacttt cgcatatacc ctcttggacg accatccgtc tgatcacgaa
     7381 gcctcagacg attcggattt gaatctcttg gcaatttagc gagagcggtg cgatctcctg
     7441 cggcttttaa ttcctgacgt ttatctgcat acttttcaat catcgccatt tgtttctttg
     7501 ctttagcaat tttcgacttt tttgccatat acctttatac tccttttatt ttgatttaga
     7561 cacctcaaat cgtaatcgtt acgttttgac actgatactt taccaacaag agttgctctt
     7621 gtcaacaata aataacatga aataaaactc atatgaatag ataactattt gaaaacgaat
     7681 ttatcggtac atagagataa tcaaatcatt taaaagaata tgcagtttag gctctgaaaa
     7741 ataagaattc acttttatta gtccaatcaa acacaaatac cgttttaagt agcttttcct
     7801 ctacttaaaa cggcaatatg aaagaaacta taacttttta ttcaatcttt tatgtttaac
     7861 tggtttatcg ccaatttttt aaaactttga ttattcctta tattataaga atagacatta
     7921 agtgaactaa ttcaatcata ctgattcttt cgtgtattca aaattagatg ctgtttaata
     7981 aaattattct ttttgaaaaa gaattataac tatatcccaa aaattgtagg ttaatttaaa
     8041 gcctatttca tcatcgtgat tgagcttctt tttcatagac gaatttctca gatagtttag
     8101 ttttctttat gagatttttg tatatttctg accttcgata caactcatta tgagtacctt
     8161 gatcaactag ttttccttga tccatcacta caattttatt taccttttca atagacttca
     8221 agcgatgcga tataattagt actgtcttat cttgtattaa acgatttaaa ctttcctgaa
     8281 tacgtttttc attttccgca tctaaactgg cagtaatttc atctaaaatt aaaattggcg
     8341 catttttcaa aaaagcccgt gctatggata ggcgttgtcg ctctcctcca gaaagtgaac
     8401 ttccgttttc tccgaccaat gtatgataac cgtcaggtag tttttcaata aagtcttcgc
     8461 agttagctaa acgagctgct tttttcactt cctcatctga cgcttgacta tttccaatac
     8521 gtatattttc caaaatactt gtattaaaca aagttacctc ttgaaaaaca atagcgattt
     8581 tgctaaacaa agaagctgga gatacacgtt taatatcata cccatcaatt aaaatacagc
     8641 cttcatcata atcatacagt ctagacacta atttcaagat actagttttc ccggaaccac
     8701 tagcacctac taacgcagtc acttctcctt gtttagccgt aaaagagata tgatctaaaa
     8761 ttggggtatt gttatcatat gagaaagaga catctcgcaa ctcaatgtca aaagatttca
     8821 gaggactatc tgatccctct tggatactgg tttccttcat agctctaatt cgctgtattt
     8881 taggagctaa ataaaatatt tcaagcactg cttctttcat agaatcgaag gaatccttta
     8941 tttttattgc ggctagtaag taaccaacga cgtagagtat agtcacttca cccgttaaca
     9001 gtaagtgaac acctactaga atgactactg ctaaactaac atatgagaaa atagaggata
     9061 aggccatgac tgaaaaagta cttaattcta cctttaaatg aatcctttca ctctcttcca
     9121 tttttttgaa aagtctgtct tgaacttttt tagatagatt aaagctatta atctcttgct
     9181 gcaattctat agtttcttga aattcttccg agttttctct caaagtatcg taatatttag
     9241 tattggcttt cgtttgggat ttctttgata acaagattaa gacaaaacta aataacgttg
     9301 gcaaaataac agctaatccc attttgacat taccaaccaa aagcatcact gaaataaaag
     9361 ggaaaaacag agccataccg ccggctttag gtattgcatg actcatcgca tgctcaatac
     9421 cttcaacgtc agacatgata gtttgagata aatctgataa attatgtttt gaaaaatagg
     9481 ataatggtaa atttgacaaa tcgtctgcaa tttgcactct taaatgcgca ctttctttat
     9541 aagtactatt atataagttt tcatactccc tatctaacaa ccaatacatg aaaagcaaag
     9601 taacaaacga tacggcaaaa taaagccaac ggggctttgt attttctaaa actaattcgt
     9661 ctatcagcat cattataatg aatgctggta ccatattaat gcagtataca aaaaaactag
     9721 aaatagaagc tttagttaaa gcttctgcgc cctgatcact taaagcaaat ttgtttttat
     9781 aaaaactttt cattgtcaag cctccaatca tttgcattct cataattagt gaccaattgt
     9841 ttatacactt gatgtttagc cattaattca ttatgatttc cacgttctac tattttccct
     9901 tctgacaaga caagaatttc atctacagat ttaatgctgg ataatcgatg agctatcata
     9961 ataactgttt tttctttcat cagatgttga aaagcctttt gtagttcata ctcattatct
    10021 ggatcaatag atgcactggc ttcatccata ataacaatct ttgcatcttt taaaattgct
    10081 ctggcaatag ctatacgttg tttttctcct cctgacagat aaactccttt actaccaatt
    10141 aaggtatgct ctctttcggg aaatttttct aaaatcgatt cgcaaccggc taatcttagc
    10201 gcgtcaaata ctttacttgc tggcgcttga ggatttgcta tagaaacgtt atcgtaaata
    10261 ctctgattga atagtttcgt atcttgaaag acaaaagcaa ttgtgttaat aattgtgtct
    10321 tttgaatagt gatttaaagg cttaccacca attttaatct gtcccttatc cacattgtag
    10381 aaacctgaaa ttagtttagc tatcgtactc tttccgcttc ctgattttcc tactaatgca
    10441 taaattcttt tttgttctaa aactaagttg aaattttcta aaacacactc ttctttgtat
    10501 gaaaagctga cattttcaaa tgcaatgtca caatttttaa tctgtgtctc gttcccatat
    10561 tttagttggt cttgcttcat ttctaaataa agggagtcta gtgtttcgac tgcatatctt
    10621 gcttcaaaca agtgcatact tacatacatt attctcatga aagaaacaaa aataattcca
    10681 cttaaaaaaa acaacatcaa caagtcaaca gctaatatct tgggagtggt aagagaagtt
    10741 ataaaaaagg ttataggaac tatcaaaatg caaactatgc caaaaaatag ccattgaaaa
    10801 agaacaaaag gtgttttccc actcaaagaa tacttatatg caaacttagc ataatcgtta
    10861 attgctttat tcaaggcttt aaatgatgtt actttagcac caaatatctt tataacttga
    10921 attccgcgga tatattctac cgtttcagag cttaacacat ctaacgattc ttgatatttt
    10981 tgaataaaat ttgtattctc tgtcattttc tttaacaaga atacaccaat tactaacatg
    11041 gcgattagaa ccaatccaat tttaaagtta atgaaaaagc ctaaggcaac tgataaaaca
    11101 ggtgttacta tggcttgagc actatctgga atcaagtgag caattgccat atgtgtttta
    11161 gccgcgttgt catcaattgt ctttcgaata tatcccgaag aatttaaatc aaaaaaacga
    11221 aaacttgctt ctgttaaacc gctaattccc ttttttctca aattagtctc taagcgaaac
    11281 ccaaggatat gagaaaataa gccagaaaaa atataaaata aagctcctaa agttaaccaa
    11341 gcaacagttt gaaccgcata actcttagct aaatattcat ctcctacaat aattagagac
    11401 tgaagaaatt tgtaaatact gtaatatcca tagactgtta atacgacaga aaaatcagaa
    11461 aataaagtgg ctaaccatcc gtagtatttc attttaggga cataactaaa taattttttg
    11521 tatactttca ttttttcctc ccccgcattc aatttcttct cttattcgtt tacaactgtt
    11581 aatgctttcc gtaatagcgt aaattagaga tgtacaaaca gataaaaggt aaactacgtt
    11641 ctattttttg tattttttta agacttttct tccaatcaat acaccaatca tagataaact
    11701 tgctacaact aacactgaaa tgattcccca tatacctacc gcatagtgga tcattgcatc
    11761 cattgttgca gcagattgtc ctttatctat ccaatcttga cgaaaacgat ctgtaaagaa
    11821 ccatatagga atccactgtc cggcagcact gcctaattgc attagagccc aagacgcaac
    11881 ttgaggaatt gctcgatcat atccaaatac agatagaata atatcagcta agatcgccat
    11941 actaatcgaa ctgataaacc aaggtaagta cgccccgccc attaacgtaa aaaataacaa
    12001 taaaacaatt gtgtaaatag taaaaattcc ttttctaggg tagttatagc taatataaac
    12061 aaatatgatt ccagataata atccaaaaat cccgggggat atgctgtgac caaacgcccc
    12121 accaatacta ctaaccatta ttgaaaaaaa gtaaattact agggaaataa ctgtagctaa
    12181 accaataaaa atataatctt ttgttttcat atatattctc ctcaattcta tttcgttatg
    12241 tcattaaaaa tctgttctat caaagttgaa tcttctactc taagttttat atcttttttt
    12301 attccaccat tttttaaata aataattcta ttgcatgttt tataaagaaa ttccggatca
    12361 tgagtagcta taactactgc acccttttct tttaagtctt taataaggtc agcaatcctt
    12421 tccatattaa tataatccaa cccacttgta ggttcatcaa atagaaataa attagttttt
    12481 gacaatgaag ctatagaaac caataatctt tgtttttctc caccggataa atcaaacggt
    12541 aattgatctt tcattttata aagttccatt ttttttaaaa ttttttcttg ctgatcttta
    12601 ctaactattt cattccccag ggctaactcg ctcctcactg attctgtgaa aaattggtaa
    12661 tccatttctt gcattactaa aaacggcgat gacaaatttt ttttattttc gattatcttt
    12721 cctttggtag ggcgaataat cttcatgagt attcttagta aggttgtttt ccccacaccg
    12781 tttttcccta ccaaaccaat aacatctcca actttgacat caaaatttat gtcttttaga
    12841 atattttcat aagtaatttc ttcaactttt aatacatctg cacttacctt ttcgatatag
    12901 ggaacttcaa tcgaactatt aaaaatgtca aatttcctta atgcgttatt gttttcagtt
    12961 tttaacaact gaatagttgt agtttttaca gttctatttt taacaaatag aacttgatca
    13021 attaattctt tcaaatagta aaaacgatgt tcagctataa caatagtaaa tcctttattt
    13081 tttaaatcac aaagtaagtc agcaagtttc ttagtcgctt tgaagtccaa atttgaagac
    13141 ggttcatcca atataattaa tttagagttt agcgataaac tacatccaat agaaattttc
    13201 tgccgttcac cacttgatag cgaaaaaatg tttttatcta atatatttga aagatctaat
    13261 aattcagcta attcattgat tcttttatcc atatgctctt ttgtaaatcc aaagttttcc
    13321 atcgaaaata ctaactcact cgttgtattt gttgtaaaga attgtcctct aggatcttga
    13381 cttacgtagc caatattttt tgatatactt tcttgtgtta aggattgtag cgagttattt
    13441 tcaaataaaa tttccccaac caattttccg tcataaagtc ttggaataag tccattcatt
    13501 aactttaata gtgtggattt cccactccca gaagaaccag taatcaatgt aagactgcct
    13561 ttttttattg aaaaattaag gtcaacaagc gcttgttcct taacatcctt atatgaaaaa
    13621 gaaacattgt taaattcaaa caattctttc acctcaaatc aaaatactta agaaaaaaat
    13681 agtaacggaa aacaggatga tatagtctaa taagctcatg tttgttttat agtaagaact
    13741 tcttttcaca ggagattcta tcccctttgt tattgcatta gcggtcattt cttcagcaat
    13801 catagttgca cgtataagaa cgggaactaa cagaaattcc aaatattgca gaggctgaaa
    13861 aaaagacaag cgaatgtttc tcatttttaa agattcttta atcattctta tttcagaacg
    13921 ataagtcgga aagaatctga tcactactgt aatacttaac attatttttt tcggtaaatg
    13981 tattttttct aaagaggata ataattcatt gctagtatag acactaacca acaaccatga
    14041 gatcattacg accggcaaca ttcgaaatac aatatataag gatgtcgcaa aaaatatagt
    14101 aaaaaactct atcttgatag catcaataat tttcattact aaaaacagaa ctgaataaat
    14161 caagatgaaa taaatacatg tctttatttt acgaaacatc aatactaaaa ctgtagcaat
    14221 aatgaaacac cccacttgaa atatatttcc ctttcctaaa aaaataatga ccggaaaaaa
    14281 aatatttaaa gtaatcaagg ttaacggatt gatttttgac actctttcac ctccaaaaaa
    14341 taagatcgca ctgttatctt aagctgataa aaatgcaccg ttattcagat aacagtgcga
    14401 tcttatcaaa aagcataact gagaacaatt ctcaatgcaa gcttttttta attaactcag
    14461 taattcaatc cacccaggaa cagaaaattg atacaatctt ctggttaata gcatagtctt
    14521 ttcttttgaa tagctatgct tcaatatatc atagaatgta gcgtagaatg agtaaatgta
    14581 gaagtggatt tcttcttcat taaagactaa attttgattg atcattccga tagtctttaa
    14641 cttatctaga caacagactg tctttttcgt cacaaatgat gtaatttttt caacaatatc
    14701 ttgatatttg gttccagctg attggaataa aatcaaatcg aatatacgtt tattctcaaa
    14761 aatataatta ataaatggat ctaattcatt gaagttctct ctccaacttt tctctatatc
    14821 tatattttct aagtttaaga ggtatttggt aaactcttca ttatacatat taataagcgc
    14881 attaatatgc ggctgaataa tttcttcaaa taacgcttcc ttagaagtaa aatgtttata
    14941 aaaagcaccc gtagttacgc ctgcggtcag gcacagttgc cttatcttag tttgctgaaa
    15001 tccttctttt tcaaaatggc ttagtccact ttctattaac ttttggtgag ttataatata
    15061 atctctggtt tctatgggta cttcctccta ataagatgaa ttaactttat tatcttacac
    15121 aggacttgaa gtttcaatcg ttaacgttta ttactgaact cattccttag agctaaacta
    15181 atgagaaaga gcagaataac tgatacaaaa agcaatccta caagaattaa tagactcatc
    15241 taaatcattc accttttcta aaataatccg agaaagattt aaaaaaatta tatccagcta
    15301 ttaggataag tcctatcata atcactccaa tagccatttt cttatcctcc ctaaaaataa
    15361 agtcttttac ttaaaacctt ttttgtaatc gtcaataaat tttttccctt tatcagttaa
    15421 ttgaaatcct atatctaaat agtcctcata tctaagttta gccagcatct cacttacaga
    15481 tgctggacta acacttagaa cttttgctaa atcattattt tttaatttct tttttctttt
    15541 tgataattcc tgaattgcta gtaaataaat tagtatagta gtcgttttaa tttgtaccat
    15601 acctaccctc caaattattt cgaaaggcct tcagcaattt tatccaggtt ccatttcatc
    15661 atcgcatagt aactatcacc atcttgtcct ttttctgcaa ttgaatcagt aaaaatcgtt
    15721 gaatagatag gaatattggt atcttttgat actgttttca ttggtctatc atccacactg
    15781 ctttctacga ataaggaggg aacttttgtt gtgcgtaatt tttcaactaa gtgttttatt
    15841 tgatctggtg ttccttcttc ttcagtgttg atttcccaaa tgtaagcaga aggcacatta
    15901 tacgctttcg agaaatattt aaagcatcct tcacttgtaa caatcatttt tttatcattc
    15961 ggaattgaag caaatttaga tttagcttct ttgtctagag aatccaattt ttcaatatac
    16021 ttatctagat tttctttata gaatttttta ttatcaggat ctttttccgc taactgtttt
    16081 tcaatatttt tagcgtaaat aataccgttt tctaaattta accaagcatg aggatcttcc
    16141 ttccctttct cactctgacc ctctaagtaa ataacatcta tgccatcact tgctgcaaaa
    16201 tagtctttgt tttcctcttt gttcgcattt ttaactaatt ttgtaaacca agcatttcct
    16261 ccagtttcca agttaacacc gttataaaaa atcaaatctg cttttgaagt tttttgaaca
    16321 tcttcaggca aaggttcata ttcgtgggga tctttcccaa taggtacgat actgtgtaaa
    16381 tcaattttat cttttgctat attttcggta atatctgcta aaatcgaatt agtaactact
    16441 acttttaatt tttcgttcga agccacttta tctttgtctt tactatttat tgaacatgcc
    16501 ccgaatataa aaagagcaat caaaatcccc agacttccca gtactgcttt ccattttctc
    16561 atcataacga tctctccttc tttcttaaaa atttttgttt tggtgagatt agaaaactaa
    16621 ttacaaataa gaatgccgct gttaaaacaa tacttgatcc tgctgctatg ttaaaactat
    16681 aaccaataaa caatccaagc aatgatgaac ttgctccgac tgttgcagaa ataaatatca
    16741 tagttttcaa gctatttgca tataaatatg ctgtagctgc tggtgtaatt aacattgcaa
    16801 caattaatat agttcccaca ctttgcatag ctgttactgc tactaaagtt aatagaacca
    16861 tcaatagata atgataaaaa ctgacattca tccctattgc ttttgccatt aatggatcaa
    16921 aagaagtgat taataattct ttaaaaaata gaactactat aaccaacact aaaaccgaaa
    16981 cgccaattgt aatccactta tcaatatctt gtacagctaa aatatttcca aataaaatat
    17041 gaaataaatc cgttgagcta tttgcaactc ctataaggat aactcctagt gctaaaaaag
    17101 aactgaaagt aataccaatt gctgtatctc cttttattac actattgctt tttataaaag
    17161 taataatgat agaagctaat agaccaaata caatcgcacc aataaaaaaa ttaataccta
    17221 aaatataaga aattgctact cctggcaaaa cagcatgaga aatagcatca cccataagag
    17281 acattcctct taaaataata aaacaaccta ctgttccaga aacaatccct ataactacag
    17341 atgtaattag agcattctgt aaaaagtgaa agtcatacag accattaata aaattttgaa
    17401 gcatattatt ccccctctcc aataaatata gtatctccat aggctttttt taaattttct
    17461 tcattaaata cagattctac acttccgtga gcaattagat ttcgattcaa aagaataata
    17521 ttatcaaaat acttctttac cttgcttaaa tcatgatgca caattagcac tgtttttccc
    17581 tgatgtttca atttttttaa cgtatccata attattcgtt cactaactaa gtctatccct
    17641 acaaatggtt catctaaaaa tatgaaatcc gcattttgag caagacatct tgctaaaagt
    17701 acacgttgaa attgtcctcc agataattct cctatttgat gattagaata ttttcccata
    17761 tcgacctttt ccaaagcctt tgaaactctt tgccattctg catttttaat tctttgaaaa
    17821 actttcattc cggcatatgt tcccatagat acacattctt ttacttttat tggaaaagta
    17881 aaatcaatat cagctttttg ctctacatat gcaatcttca ccagtttttt attgatatct
    17941 tcattattaa tggagacctc tccctgatga gggataatat ttaacattgc tttaagcaat
    18001 gtggattttc cagcaccatt tggaccaata atacctgtta aagcaccttc ttgtattttt
    18061 aaagaaatct cgttcaatgc taaaaaatcg ttataacaaa cattaagctt tttcacttct
    18121 aacataactt tctccttcta aatgataatg attatcacga tcatatggta tacaaaaatt
    18181 atattttagt caaccctaaa ttttatagtt tagatttttt tctgttgctg aagattagaa
    18241 tgacaaaact atttgaaact acgcgtatag taagtagctt tcaaaatata taaaaaagga
    18301 gcgttctcaa atgagaacat gctcctaaaa ctactttatt ggcgtatgga aaaattatac
    18361 ttgaagtgga tagcgggttg aataagttga taagcatcaa aacatttata aaaattattc
    18421 aagactatgt ctgttccata tacaaatatg aaaatcagta aactccattt aacttttttc
    18481 gtagtgtgtc tttttataca tgtttatcgt tattaatagt gaataggaaa aaaactattt
    18541 ttgatcattg acaattaaat acaatttttg aataacgatc cctgctattc gagctagaaa
    18601 ctcaagtacg ccatgacctg attagcgaca aatacttaga gatttctttt tgttctggga
    18661 aaacaaaatg ccatgacaat agcagcaaat aatgatactt ccgtaaggct cgccgtaaac
    18721 tggggagagg tgaaattcct atgtgcttac ccaaagcaat tcaaaaattt cataactact
    18781 attttaggag atgattttat agaatccaga gtctacctga ataaaaaaca aacctgtgca
    18841 tttctaggaa tcgataataa tactctagat aagtggattc gtatatataa tttcccagta
    18901 ataagagttg gtggtgtagt tcgctttgac gtagaagata ttaaagactt tatgaataca
    18961 tttaaaataa cttgaaattt tcttctcgcc gcatatacta aaattagtga aatatactgc
    19021 cttacttttt ttaaggagtg aggattttgc ctacaaagct ttcaaatggg aaatataaaa
    19081 ctaatctgag atatccaaaa aggtttaaag aaataacagg aattgcatca gaaaagtatc
    19141 aaaaaacatt tccaaatagg caattggcga ttaaagccga aaatgatatg aagaagaaaa
    19201 tagaaaaagt tttgagagag gagaatgcaa actcattaga attaaaagga aaaataacgt
    19261 ttaaaaaatt ttacgaatct aaatggcttc ctagatatga attaggtcaa acaattagat
    19321 caaacaggcc tcctagtgat ataaccatta gtaatacaaa agatatattt cggttgcata
    19381 ttttacctat gtttggtgaa tacgcaatga attatctaaa tatcaatact gagataatat
    19441 cagatgaatt aaccaaaaaa tcgaaagaat atgcaaatat aaaaatcata aaaggatatg
    19501 ttcgttccat gttcgatatt gcagaaatac taaattatat cgaatttaat cgaactacta
    19561 aaattattca aagtatcact gcacctaaaa aaaatgcttt agaagaaaaa agaatacaag
    19621 aaggtaagca agcactatca tcaaaagaac ttactaattg gatagaagca gtaaatgatg
    19681 attttaataa tcatttactt acttttcatg attacacact ttttatgatt accctgtatc
    19741 ttggtgatag aaaaagtgag acttacgctt tgcaatggaa atatattgat tttgaaaaac
    19801 aaacggtccg tcttaaacat actttagata aatatcaaag aaaaaagttt acaaaaggtc
    19861 gaaaagatac cgtcattcaa gtaccagaag ttgtaatgac attgctaagt gaatggaaat
    19921 cggtacaagc tgatcaattg ttgaaattaa aaattaagca aacgttagat cagtatctct
    19981 ttacttatac taaaccatct ggagaagtaa attgtcctgt tcatgccgat tacctaaact
    20041 atcgaatcaa ttcaattaaa agaaggcatc ctgacttagt tcatttatct ccacacaaac
    20101 tccgtcatac ttatgcaact atagctagac aaggtggagc agatatgaat caaatttcaa
    20161 atgctttaac acactcagat atttctacaa caaagatata tgtgaacaca ccagatatag
    20221 ttgataaggc tgtttttgaa gcttttcaga gaggattgaa taagtgtgat tgatatgtgt
    20281 tcttaaatgt gttcttaaaa acaaaagaag cactttccaa ataattgaaa agtgccagaa
    20341 acgttgatat ataaaggata ttaacgtttt gagaattgtg aagctttacg tgctttctta
    20401 agacctggtt ttttacgttc gaccatacgt gcgtcacgag taagtaagcc agcacgttta
    20461 agagctaaac ggaagtcagg atcaacttct agcaatgcac gagcgatacc atgacggata
    20521 gctcctgatt gtccagcgta tcctccgccg tttacgttaa caaatacgtc atatgcgcct
    20581 tttgtttcag taacagcgaa aggttgattg attacttcac gcaagtcagc atgtggaatg
    20641 tattcttcaa catctttttt atttacagta attttgccag ttcctggtac tagacgtacg
    20701 cgagcaactg cgtttttacg acggcctgtg ccgatatatt gaacttgtgc caatgaaatt
    20761 ccctcctatt agattaagtt tgtgatgtct aaaacttctg gttgttgtgc tgcatgtgga
    20821 tgttctgctc caccgtatac gttcaatttt gtaaattgtt tgcggcctaa agtgttttta
    20881 ggaagcatac cttttacaga agtttcgatc aaacgacgag aatttttagc acgcaattcg
    20941 ccagcagtga ttgatttcaa tcctcctggg tagttgctgt gacggtaata aattttgtca
    21001 gtcgcttttt tacctgttaa tttcactttg tctgcattga tgacgatgac gaaatcgcca
    21061 gtatccaaat gaggtgtgaa agttggttta tttttaccac gtagtacaga tgcaacaact
    21121 gttgaaagac gtcccaatgg aacatcagtt gcgtctacta cataccattt acgttctact
    21181 tcgcctggtt tggccatata tgttgtacgc acgttatttt cctccaattt aagttctgat
    21241 gtttgacata cacttcgagt ttccggggct ctttgtgggg caaacaatac catttaacat
    21301 attacccgct aatctagtca atgtcaacgt ttttctgctt gattgaaaaa tttatatgaa
    21361 actttacttc ttttctttct tttatataaa agggtcgttt cctgtcaaaa tgaaaaacaa
    21421 aaagaggctg tgacaaaagt cttgtcacaa ccccttattt tgaataaacg gtgttctcct
    21481 gccaactcct cggtcacaag tcacaatatc cataaaacat aagaagctgt tttttaaaat
    21541 tgtttcttgt gactcggagc tgaacggcag gct
//
LOCUS       DACEEZ010000045        20828 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20313_2017
            SAMN12639966-rid9879223.denovo.045, whole genome shotgun sequence.
ACCESSION   DACEEZ010000045 DACEEZ010000000
VERSION     DACEEZ010000045.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639966
            Sequence Read Archive: SRR10023158
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 20828)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20828)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741683.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741683.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:44:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,016
            CDSs (total)                      :: 2,962
            Genes (coding)                    :: 2,889
            CDSs (with protein)               :: 2,889
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 36 of 73
            Pseudo Genes (incomplete)         :: 29 of 73
            Pseudo Genes (internal stop)      :: 18 of 73
            Pseudo Genes (multiple problems)  :: 8 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20828
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639966-rid9879223.denovo.045"
                     /isolate="E20313_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(<1..257)
                     /locus_tag="IWO89_001680"
     CDS             complement(<1..257)
                     /locus_tag="IWO89_001680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0456428.1"
                     /translation="MERQESIREVQEVPTEQAKETQEGRSFSYGKNFSFLRPSIQISV
                     FVLWRIHNNRYSVGSQLFYQEICHALSKKYSQNAYKEALAF"
     gene            complement(273..923)
                     /locus_tag="IWO89_001681"
     CDS             complement(273..923)
                     /locus_tag="IWO89_001681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0456429.1"
                     /translation="MKKRFIYVCVGGLFLFGVGSTFFLIHEKLQLTAKEEKLTVEINK
                     FQEEKKGQEDLVTSLKEQLQQVESEVTTLKKANEELKNNDSTEKGNIQTFQKTVTEAF
                     EVLFNYEPDSFQERKNNCAPYLSDKLMKQYFKEGVSYGDSNGVSSELEKCSVYLKSTQ
                     KATIQGLVVVSYRNKIGDSSWNNAMNIFDVTFDPASKKITFMQNLGSSFNQQLLEE"
     gene            complement(934..2106)
                     /locus_tag="IWO89_001682"
     CDS             complement(934..2106)
                     /locus_tag="IWO89_001682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHAP domain-containing protein"
                     /protein_id="HAQ0456430.1"
                     /translation="MKKKLLLWCGFGCLPTLLLCMFGFLFMILLISSGSEEEEIDCSP
                     TEIVASTISLTNQGMETNGLQIAKEVQKHIPNTTLEGLAGMLGNFQQESSMSPTAIER
                     PNDPLSGHGIAQWTAGRTTALKNFAKERNKEWSDLGVQVEFLIHELNTTEKASQVALQ
                     AKSVEEATEQWQVKFERAGIPMMGNRITFANQWYAKLGKNDSDPVASITLENGANAGS
                     KDLTDYCLETTGTSSSSDSNVLNVAKSLIGYFHYSQPQRAQFGSVENPDKNGYADCSS
                     FVWLVLTKAGYKTPANVGWFTGSMTSDARGSKQYLQEIPESEAKAGDIIIVNLGSGVG
                     NDGHTAVLAEDWHGLSTKVIEQGGMNTNGVGEGQVNLSFGYLLNGGDICFARPIKK"
     gene            complement(2125..2997)
                     /locus_tag="IWO89_001683"
     CDS             complement(2125..2997)
                     /locus_tag="IWO89_001683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HAQ0456431.1"
                     /translation="MKKMILGSLFSATVLLGMVGTSAYADTTSPGDPSVPPTEIIPPT
                     EETPEVPEEPVLPPTDGGEETPEIPEIPETPETPEIPPTEENPDTGGGTETPEVPENP
                     DTGGGEGTPEVPPVEENPGNGGNTETPEKPSNPGGNEGNTTPPTTPPTNNGGTNTTPP
                     VQDVVVKPDGSIGSQQTGGQNVQIETNNASELTHIPTPSTPIETASGEKIVSVVDGVA
                     YKESGNGLTPISDKVEQLPSGNIAVKGSDGKMKVLPKTGEEVSLVATALGGLLASGSG
                     FAFWRKRKATQKMN"
     gene            complement(3019..3939)
                     /locus_tag="IWO89_001684"
     CDS             complement(3019..3939)
                     /locus_tag="IWO89_001684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0456432.1"
                     /translation="MMMIRFTEVDNMSLLTDKKIALDKAFIQLKRENEELLARAEIGW
                     CTLEIYTENHEIPVFEGSYDFPVEEFHLISLIEQSLTENGQLSEEAQTFIQTLNSELP
                     KFYQVKKDQKNERRPTKIKKEKKTPLSVETAFTQSNGSLQKKKMSFQWLLFGFIVVVS
                     CGLFIMQLSTQREIEQLTKKEHQLQERIAFAEELATKQPQIDTFSRYFITNYYSGEMA
                     EERYRKKLQSFLTEKVAKQISPQKEKVKSILSWEMKKEGKHWQIAYVITLETEKKTQE
                     TKKVSFQLEEQEKNYLVSTLPELDDFEINQ"
     gene            complement(3939..4208)
                     /locus_tag="IWO89_001685"
     CDS             complement(3939..4208)
                     /locus_tag="IWO89_001685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0456433.1"
                     /translation="MIIVTGIVLLGIGVLFGYHFAYFPKIGEWQQRQAIQKYFQVKGN
                     EYTYYAEDEDSYIVSLNNQEYRVKFSLNNPIQVVYAVEVVEEVFD"
     gene            complement(4205..4585)
                     /locus_tag="IWO89_001686"
     CDS             complement(4205..4585)
                     /locus_tag="IWO89_001686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF805 domain-containing protein"
                     /protein_id="HAQ0456434.1"
                     /translation="MITNYKKFWQNSFNYKGKATRKEYWLVALMNALIGFGLGLAIGL
                     AGALNASETIIGCLIGVLIVYCFVVLVPSLSLSVRRLRDAGFHWAFIFFYFVPTIGSL
                     VLLVLYCLPTKESIEDKQVQEETR"
     gene            complement(5052..6515)
                     /locus_tag="IWO89_001687"
     CDS             complement(5052..6515)
                     /locus_tag="IWO89_001687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HAQ0456435.1"
                     /translation="MKKTSKCLSALLLVGQVFGYTPAVLANEQGNNQLTNSQSATITE
                     ETTTNSTGEENQPETPTEDTIDTPDNPEMNAPPTGSEEQPETPNQPDKPEDNQQPEEE
                     PSEEPNEQPSDESSQTPQKPSTETEAEPPTKTERPQKAPEQSVTVPMNDVPQAQSIGS
                     EETKNYHQAPSAPIQELVPSSTQDNEGTIHFEKDASVETFIRKIGESARKIGQENDLY
                     ASVMIAQAILESASGQSQLAQAPNYNLFGIKGTHNGKGVSFATQEDLGNGTLYTTQAT
                     FRQYESYEESLKDYAKLLKEGLVGNTSFYDGVWKSKAKTYQEATKFLTGRYATDTSYD
                     KKLNGLIETYDLIKYDKEVAGPQLSKEGYMVPLKNYSISSSFGWRGEEFHRGLDMAAN
                     QGEPIYASKAGTVIEASYHTLSGNYVTIEHEDGSVALYAHQQEYCVNVGQTVEQGQII
                     GYVGSTGNSTGPHLHLELALDGSLTKDKLINPETVLF"
     gene            complement(6579..9596)
                     /locus_tag="IWO89_001688"
     CDS             complement(6579..9596)
                     /locus_tag="IWO89_001688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HAQ0456436.1"
                     /translation="MFKRMATWLAIFVTFLNPMLGSVTAFAETINESNSTEEVMKTEE
                     SKSSEEAISTETTSETTEETTTQDTTEDVSPPNVMTQEVPEGYEKYFITIEDIQKATS
                     FSEQERLAQESEEWQAILEKNAQTRATNLTLTENGRKDVNMTRITPEGTMYTHDYMVR
                     WQIDGEDVFCIQEGAITSAGIQYDVTPLENLITTSAIRKKISLIGYFGYYSSGSMDNY
                     VLAQTYIWEVLGGSFVNYGTIGQSNYEAFKQTVDRKINNLLEDPSFDSQTVTVKVGEE
                     VEVKDTKGVFSQWDGSVVENTANVQVRKSGNSLFIKANQNSNDGRISVSSLDPNSRGV
                     GSSFAYVDPVAQNLGKLYVPDPNRAGLTIKVLKNGDFKLTKLDAVTGKKVPNVTYEIK
                     RHDTGETKQYTTDAKGEIALKEIPAFYEKDGKYVQNKLDVKEVKAPAGYILDKQTHTI
                     TIEPNKEVTLTAKNDLQQFIPEGKKVKEVYNAEESWNQGKPVYDTVPAENIEFDIIAK
                     TDITAPDKETVLVKKGEVLDSVKTDKDGHWKANKKFYNGDQNKYQLIEKNVPDNYRQP
                     SEEQTTFSVAYGDNTTPVLTFELDVIDNLLKTGEVDFNKVDSINHLQLPGAKFLIEGM
                     TAHNKNVSFVYETKEEPEKLKLPDGTYKFTEIVFPEHYGQASGQTETILVTVKDGEVQ
                     PITVKNEEIVVPEEDQPEISTLFVTTTGGKDFNPKKDQELVDKVSQVFPKSAVGTEKY
                     WVTQFHKITADGKVTVLDTVKETRKVTDEKETFDVTFNYKADMVSDGDTIVATHIVYN
                     DKEEEEEFARHFDLTNKDQTVTAKEPKIETRFITVNGDQTFDPDKDQELIDHVSQEFP
                     ESTVGETKYWVTQFHKIDKNGKDTVVGTVETARKVSKASEEFKVSFDYKAGTLKDGEK
                     LVATHIVYKDKDHKEEEARHFDLKNEKQTLTAKGIKKETPTTPVKQTPTSTPKQSLPK
                     TGETVNDVLIALGLFLFVGVGAVLVRRKMKQSN"
     gene            complement(9747..10586)
                     /locus_tag="IWO89_001689"
     CDS             complement(9747..10586)
                     /locus_tag="IWO89_001689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HAQ0456437.1"
                     /translation="MKMLKVLALGIVVGGVSLAVGTNAEAAENKVWTPRSAEQIKAEI
                     KGNEYTIVWGDTLSAISQATNITVQKLADMNKITNVDLIYAGNKLVFNGNVASVQNSA
                     GETVAQTVIQEKDKVDPNQPVGQGTTSNTQTTPNTTTPTTPAGDQTGTTGGTTTPSTP
                     ENNGGTTQTPTQPGNGGNTGGETTPSNPSTGGGTTTPEEPSNPGGGDQGGNGGTTTPE
                     EPTIVVGEIGNSGKVFNTVDEAYDWANAEVSDPNSQWYNKGFMVDVIIYSDGSQKHTV
                     DFY"
     gene            10731..10976
                     /locus_tag="IWO89_001690"
     CDS             10731..10976
                     /locus_tag="IWO89_001690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014862476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0456438.1"
                     /translation="MQLNNLNEIHDWAQNNLLNRKQAAKITGQNYDAFSSSLRRNNIK
                     PFLEIGDEPGPSIIRLYLREDIEKYAKQLEEKRQKRQ"
     gene            11039..11461
                     /locus_tag="IWO89_001691"
     CDS             11039..11461
                     /locus_tag="IWO89_001691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014862359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0456439.1"
                     /translation="MNKKYIVSVGSIVLIVLAIIAFSVSNKTETQPQNTVQSTTTSQS
                     SDHSVYPSLNSLKETHGQDLIDLGSSQITTVLFEKKNIAFITPKEGTDLEFSIKVTNT
                     KENLFSIPDIQYTPNKLKIGDTFGLAKVNEQYFAYSLK"
     gene            complement(11555..11872)
                     /locus_tag="IWO89_001692"
     CDS             complement(11555..11872)
                     /locus_tag="IWO89_001692"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0456440.1"
                     /translation="MPVKGTSNFDFEIFYVDDEICGVFNKQKYTKEQAIEAWKNELFD
                     PEEEISFVVDESFVGWYHGNAEEEEVHYAGWWLEEGEPREQSVPVWVISENPLEEIEK
                     LEK"
     gene            complement(11899..12105)
                     /locus_tag="IWO89_001693"
     CDS             complement(11899..12105)
                     /locus_tag="IWO89_001693"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0456441.1"
                     /translation="MNWLKNEAELYVFHEEMQEKHLVPNVRNYLFWLEEKNDAQVFGK
                     SAQSPKIKLQLTSKQWNEHEWYIY"
     gene            complement(12086..12268)
                     /locus_tag="IWO89_001694"
     CDS             complement(12086..12268)
                     /locus_tag="IWO89_001694"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0456442.1"
                     /translation="MTKCERVSVTISFIALLLSVISLLNTAIFIGQVEKQKEISLSEW
                     RYEEVNEGMNNELVEK"
     gene            complement(12282..12632)
                     /locus_tag="IWO89_001695"
     CDS             complement(12282..12632)
                     /locus_tag="IWO89_001695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0456443.1"
                     /translation="MKKSYKVFENNEIKEQEAIILSAQTPEEALEQALPFLVESQNIV
                     VEELPEFDSKIVFSFNCKKESEISVSPEFYCRSDISVEGLVMCASAFIEIFANHLQIS
                     VKELLDLLQEITNE"
     gene            complement(12658..12960)
                     /locus_tag="IWO89_001696"
     CDS             complement(12658..12960)
                     /locus_tag="IWO89_001696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0456444.1"
                     /translation="MLSKQEKKYIRQKMYAMIGNRAITELSSEELLQVQQLAQIIGSN
                     SIFDSNPLPEMTLETLTAKRYKELRAIGYRMIDIRRALRISDTKFREWRTSNGLAN"
     gene            complement(12963..13196)
                     /locus_tag="IWO89_001697"
     CDS             complement(12963..13196)
                     /locus_tag="IWO89_001697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0456445.1"
                     /translation="MTISVGNKVKYIGTAIPRYTNKLVVVKNILVNGLILEYPKEDRR
                     KVVVEGNGTWNMNSFICGFTEVEVTNDNENRSG"
     gene            complement(14182..14504)
                     /locus_tag="IWO89_001698"
                     /pseudo
     CDS             complement(14182..14504)
                     /locus_tag="IWO89_001698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347542.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(14627..15742)
                     /locus_tag="IWO89_001699"
     CDS             complement(14627..15742)
                     /locus_tag="IWO89_001699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0456446.1"
                     /translation="MATKRFTATRKYNELYYQFPKVFITSEKYRSLSDKAKIAYMVFR
                     SRLDLAIQRNQVDSQGFVYFEFTEQELANALNCCRRSVNTIKKELKEYELLEIEDMGF
                     DQQTGKRNKARLYLGELDTTENDVYSLGPVDSTSVDSAQGKEEEESVDNLAIFDDFDE
                     KNPCENSAQLFNNSSNNLDTENLDTKIDTTSPVEMADDWQKEFIAHYSESTFVPKDVL
                     TWIVRSSAEWREAKEQLNMIYDSKRIIEKRQRKYNQTHGLSDGSEYRILGETFAEDLS
                     KTYQRCIQNEKIARHEGKAIQNRKGFYFRALLNFWECCVRELNLDPMATKLSDQAEYS
                     LLQAKRSCSYEDLETQIYGLSQLPKISMHNWLEELDT"
     gene            complement(15748..16146)
                     /locus_tag="IWO89_001700"
     CDS             complement(15748..16146)
                     /locus_tag="IWO89_001700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0456447.1"
                     /translation="MVKNKFRATDVLTQATFHMPKLFFIKSSKYYAMTAQAKYAYSLL
                     NEYLEESLIEKRIDEKKHVYISYTNQELAILLNVSEEEIEEIKNELEDYDLLTIEFGR
                     IYLKELIATMEDVRVYQHQNSFLIYGQDEV"
     gene            complement(17175..17561)
                     /locus_tag="IWO89_001701"
     CDS             complement(17175..17561)
                     /locus_tag="IWO89_001701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4064 domain-containing protein"
                     /protein_id="HAQ0456448.1"
                     /translation="MNKRKTEFILILISGITGILCSLLALMGGMFLSSIDSTTLNTTE
                     LTSKDIDMLVNLSGNVVLFGGITLVFSIMIFIIGFLVRKNQKTVVYGISLLILSIIPF
                     FLLTLLWVIPGVLGIIAGLMLLIRKS"
     gene            complement(17558..18079)
                     /locus_tag="IWO89_001702"
     CDS             complement(17558..18079)
                     /locus_tag="IWO89_001702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RDD family protein"
                     /protein_id="HAQ0456449.1"
                     /translation="MNKKSGIETLPNYVFAGFWLRFFAFLIDLICISIITHATIGLFY
                     KIMGLESDKSIITFYGIASLAIYLTYFVLLTKLNSGQTIGKMIFGIRVVSFEEKELSW
                     QTIFIRECICRFILKNFVFQIGYLVAAFTPKKQHIGDYFSNTSVVVIRSLEAINYGQK
                     ISNSKTLNLERIK"
     gene            complement(18081..19115)
                     /gene="sppA"
                     /locus_tag="IWO89_001703"
     CDS             complement(18081..19115)
                     /gene="sppA"
                     /locus_tag="IWO89_001703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptide peptidase SppA"
                     /protein_id="HAQ0456450.1"
                     /translation="MNKRRWIAVGVACGLLLLSIIVALIPGKDKEEASNTTLTGINKI
                     FYGSNEITEETLEEGASNKKIVKLSVNGVIADTGESNLFSREQYNHQNFLTQLKKIQE
                     DKAVKGVLLEVNSPGGGIYESAEIAKEMAKIKKLDIPIYTAFKNTAASGGYYISAGSD
                     KIFATEETTTGSIGVIISGLNYSGLLEKLGVTDATYKSGALKDMMSPQHKPSEEENKV
                     IQEFVMSAYDRFVNVVAKGRNMDTNAVKELADGRIYDGNQAVENGLVDQIGYSEDALD
                     SLKKEKKLTDATVIEYKNDTTGFASSWLGNKIAEWQGLKATTSDRVISVFEKLGFSDT
                     PKPMYYYGGL"
     gene            complement(19148..20563)
                     /locus_tag="IWO89_001704"
     CDS             complement(19148..20563)
                     /locus_tag="IWO89_001704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HAQ0456451.1"
                     /translation="MVSIKNYGKSRRGKQFMQNNNWFEHSSIYTKQHHSFYRWVLYPV
                     ILFLCIIGLFLTFAKKEVVIRTTAQLNPEKIEKLQVPLEAKIMENFLKENQFVHKDDV
                     LVRLDCSLIENEKAQIEQENQRITQQIKMAQLFIESISKGKNLFSTDDSFGYSNQLKS
                     MLSEKESLRYALKQSELNDQKQLEVYEKTKRQLEKQIESSDSKLQEWQQVQVAWSNNQ
                     SLKDFSKEMMANYENWQEQLNNVSDDQKNQVKLTISASINEQIEQLKKEVEQYQSEKA
                     KLVKPTTSENDRISQTEKGKQELEQAITTVKQTVTELNEKQEKNQSVIKSLDDQLSKG
                     ILKAPVSGTVHLNEETKGQAELAKGTVLAEIYPIHEKSKMKFTALLPANESTYIKEGM
                     KVHFKLDQKGGAPITIDGTLDEISENSTATEKGVFYAVKGTLKTTQSFPFRYGLTGEL
                     SLIVGEKTYWQQIVDVFLNKK"
     gene            complement(20532..>20828)
                     /locus_tag="IWO89_001705"
     CDS             complement(20532..>20828)
                     /locus_tag="IWO89_001705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002393091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAQ0456452.1"
                     /translation="NLSGGQRQRLALARALLKNPDILILDEATSGLDTLLEHAIMNYL
                     LNLKEKTIIFIAHHLPIAKACDQILVLHEGHLVEKGTHDELRFADGIYQKLWEI"
BASE COUNT         5708 a         4262 c         3155 g         7703 t
ORIGIN      
        1 aaaaaggcaa gtgcttcttt gtaggcattt tgactgtatt tttttgataa agcatgacaa
       61 atttcttgat aaaatagttg actgcctacc gaataccgat tattgtgtat ccgccacaaa
      121 acgaacacac tgatttgaat cgacgggcgt aaaaaagaaa aattctttcc ataagaaaaa
      181 gaacgtcctt cttgggtttc ttttgcttgt tctgttggta cttcttgtac ctctcttatt
      241 gattcttgtc tctccataac cttctccttt tcttattctt ctaaaagctg ttgattaaag
      301 ctacttccca aattttgcat aaacgtgatt ttctttgacg ctggatcaaa ggttacgtca
      361 aaaatattca tggcattatt ccaggaacta tcaccaattt tattgcgata cgacaccaca
      421 acgagccctt ggatagtcgc tttttgtgtg ctttttaaat aaacagaaca tttctctaat
      481 tcagaagaca ccccgtttga atcaccatag gaaacgcctt ctttgaaata ttgcttcatc
      541 agtttgtcag aaagatatgg tgcacaattg ttttttcgtt cttgaaaaga atcaggctca
      601 taattaaaca gaacctcgaa cgcttctgtc actgtttttt gaaaggtttg aatgtttcct
      661 ttttctgttg aatcattgtt tttcaattct tcatttgctt tttttaacgt cgtgacctcg
      721 ctttctactt gttgaagttg ttctttgagt gacgttacaa ggtcttcctg tcctttcttc
      781 tcttcttgga acttgtttat ctccaccgtc agtttctctt cctttgctgt tagctgtagc
      841 ttttcatgaa taagaaaaaa tgtactgcct acaccaaata agaacaatcc gcctacacaa
      901 acataaataa atcgtttctt catttgacac ctcttatttc tttatcggtc gagcaaaaca
      961 aatatcacca ccattaagca aatacccaaa ggataaattg acttgccctt cacctacacc
     1021 gttagtattc attccccctt gttcgattac tttcgtagaa agtccatgcc aatcttccgc
     1081 taaaacagct gtgtgtccat catttcccac accagaacct aagttcacaa tgataatatc
     1141 gccagctttt gcctcgctct caggtatttc ttgtaagtat tgcttacttc ctcttgcgtc
     1201 gctcgtcatt gagccggtaa accacccaac gttcgcaggc gttttatacc ctgctttcgt
     1261 taaaacaagc cacacaaagc tcgaacaatc ggcatagcca tttttatctg gattttcgac
     1321 actaccaaat tgtgcccgtt gcggttgtga ataatgaaaa taaccaatca gactttttgc
     1381 tacgtttaat acattactat cggaagaact gcttgttcct gttgtttcta gacaataatc
     1441 ggtcaagtct ttacttcctg cgtttgcccc attttctaat gtaattgaag cgacgggatc
     1501 actgtcattc tttcctagtt ttgcgtacca ttgattcgca aaagtaatac gattccccat
     1561 cattggtatc cctgcacgct caaatttcac ttgccactgt tctgttgctt cctctacgct
     1621 ttttgcttgt aatgcaactt gtgaagcttt ttctgttgtg tttagttcat gaatcaaaaa
     1681 ctcaacttgt acacctaaat ctgaccactc tttgtttcgc tctttggcaa aatttttcaa
     1741 agcagtcgtt cgccctgccg tccattgtgc aatcccatgc ccagataatg gatcgtttgg
     1801 tcgttcaatc gctgtgggac tcatactcga ctcttgttga aaattgccta acatacccgc
     1861 tagaccctct aatgtcgtat ttggaatatg tttttgtact tcctttgcaa tttgaagccc
     1921 gtttgtctcc atgccttggt tcgttaatga gatagtactt gcgacaattt cggttggact
     1981 acagtcgatt tcttcctctt cagaaccact actaatcaaa agtatcataa acagaaaacc
     2041 aaacatacat agtaatagtg tgggtagaca accgaaccca caccataaaa gaagtttttt
     2101 cttcatgttt gatccttcat tcgcttagtt cattttttgt gttgcttttc ttttgcgcca
     2161 aaaagcaaaa ccgcttcctg aagccaataa tccaccaagt gctgtcgcaa cgagagacac
     2221 ttcttcgcca gtcttaggca atactttcat ttttccatca gaccctttca ccgcaatatt
     2281 tccgcttgga agttgttcga ccttgtctga aatgggcgtc aagccatttc ctgattcttt
     2341 ataagcgaca ccatcaacga cggagacaat tttttctcct gaagctgttt caattggtgt
     2401 acttggtgtt ggaatatggg ttaattcaga cgcattattg gtttcaattt gaacattttg
     2461 tccacctgtt tgttggctgc caatacttcc atctggtttt actacgacgt cctgtactgg
     2521 aggcgtagtg ttcgtccccc cattattggt aggtggtgtc gttggaggag tcgtatttcc
     2581 ttcattgcct cctggattcg acggcttttc tggtgtctct gtattaccac catttcctgg
     2641 gttttcctca acgggtggaa cttcaggtgt tccttcccct ccgccagtat ctggattttc
     2701 tggtacttca ggcgtttctg tgcctccacc tgtatctgga ttttcttccg ttggtggaat
     2761 ctctggggtt tcaggagttt ctggtatttc gggaatttct ggggtttctt ctcctccatc
     2821 tgttggtggt aaaacaggtt cttcaggaac ttcaggtgtt tcttccgttg gtggaataat
     2881 ttctgtcggt ggtacacttg gatcacctgg tgacgtagta tctgcatacg cactggttcc
     2941 taccatacct agcaatacag ttgcactaaa taaacttcct aaaatcattt ttttcatgtg
     3001 tttttcctct tctcttattt attgatttat ttcaaaatca tctagttcgg gaagcgtgga
     3061 aactagataa tttttttcct gttcttccaa ttgaaaagac actttttttg tttcttgtgt
     3121 ctttttctcg gtttccaatg taatgacata cgcaatttgc caatgcttgc cttctttctt
     3181 catttcccag gataaaatac ttttgacttt ttccttttga ggactgattt gtttcgcaac
     3241 tttttccgtt agaaacgatt gaagcttttt cctataccgt tcttctgcca tttcacccga
     3301 ataataattc gtaataaaat aacgactaaa tgtatcaatt tgtggttgtt ttgtcgcaag
     3361 ttcttccgca aatgcaatgc gttcttgtaa ctggtgctcc tttttcgtta gttgttcaat
     3421 ctctctttgc gttgaaagtt gcataataaa aagaccacaa gatacaacga cgataaatcc
     3481 aaacaaaagc cattgaaaag acattttttt cttctgaaga gagccgttgc tttgtgtaaa
     3541 cgctgtttct acagaaagtg gtgttttctt ttcctttttg atttttgttg gtcgacgttc
     3601 gttcttttgg tctttcttta cttgataaaa cttcggtaat tcggaattta aggtttgaat
     3661 aaaggtttgt gcttcttcac ttaattgccc attttctgtt aaagattgtt caatcaatga
     3721 aatcaaatga aactcttcta ctggaaaatc atacgagcct tcaaatactg gaatttcatg
     3781 gttttctgtg tagatttcca aggtacacca gccaatttcc gctcgtgcca atagctcttc
     3841 attttctctt ttcaattgaa taaatgcctt atctaatgca atttttttat ctgttagtaa
     3901 agacatgtta tcaacttctg taaatcgaat catcatcatt agtcaaacac ttcttccacc
     3961 acttctaccg catacaccac ttgaataggg ttatttaaag aaaatttcac ccgatattct
     4021 tgattgttca aactgacgat atagctatcc tcatcttcag cataataggt atattcgttg
     4081 cctttgactt gaaaatattt ttggatcgct tgtctttgtt gccattcgcc aatctttgga
     4141 aaataggcga aatgatagcc aaataaaacg cctatcccta ataaaacaat tcctgttact
     4201 ataatcatct ggtttcctcc tgtacctgtt tatcttctat tgactctttt gtcggtaagc
     4261 aataaagaac cagtaacaca agagagccaa tcgttgggac aaaatagaaa aaaataaatg
     4321 cccaatgaaa acctgcgtct cgtaaacgtc gaacgctgag agaaagagac gggacaagaa
     4381 ctacgaaaca atacacaatc aatacgccta tcaaacaacc aattattgtt tcagaagcat
     4441 tcaacgctcc tgctagtcca atcgctaagc ctaggccaaa cccaatgagg gcattcatta
     4501 acgcaacgag ccaatactct tttcttgtgg ctttcccttt gtaattaaag ctattctgcc
     4561 aaaatttttt ataatttgtg atcatatctt tgtcctttct aatgtttatt tttgattcta
     4621 gtttcttctg ttttactaac aatgaaactt gccataaaaa aggtggccaa tacaccacca
     4681 gctagaaatc cacttacaaa taacaaaatt ttttcctcct ttctaaaaaa tttcgccata
     4741 aaaaggcaaa gtatgaacac gataaatacc atgtacatac tttgcctttt tatgcaatat
     4801 atgttaactc tccaagcaat tactgatttt taaattcttt ataaataaaa taaaaatcgt
     4861 ttgagtaaag aaataagtca atgaagaccc aaacgataaa aaatattcct aatactatat
     4921 ttaataaact acgttctaga tagatacgag atttcaaatt aaaaaacgaa aatgagagat
     4981 acaaatatat caaatattta aaaagtaacc acattttcta taccactcct tatcagaaac
     5041 agccttattc tttagaataa gactgtttct gggttgatta atttgtcttt tgttaaacta
     5101 ccatctaagg caagttctaa atgtaaatgt ggtcctgtac tatttcctgt tgaacccaca
     5161 taaccaataa tttgtccttg ttcaactgtt tgtcctacgt tcacgcaata ttcctgttgg
     5221 tgcgcataaa gagccacact tccgtcttca tgctcaattg tgacataatt tcctgacaaa
     5281 gtatgatacg aagcttctat gaccgttcct gccttgctcg catagattgg ttctccttga
     5341 ttggctgcca tgtctaatcc tctatgaaat tcttctcctc gccaaccgaa ggaactagaa
     5401 attgaataat tttttagagg taccatatag ccttctttgc ttagttgtgg accagctact
     5461 tctttatcat atttgattaa gtcataggtt tcgattaacc catttaattt tttatcgtag
     5521 ctagtgtctg ttgcgtatct tcctgttaaa aatttagtcg cttcctgata ggtttttgcc
     5581 ttacttttcc aaacaccatc ataaaaagag gtatttccaa ctaagccttc ttttaacagt
     5641 ttggcatagt cttttaagct ttcttcatag ctctcatatt gccgaaaagt tgcttgggtc
     5701 gtataaagtg tcccattgcc taaatcttct tgtgtcgcaa aagagacgcc ctttccgtta
     5761 tgtgtgcctt tgattccaaa tagattgtag tttggtgcct gagccaattg actttgtccg
     5821 cttgcggatt ctaaaatggc ttgcgcgatc ataacagaag cgtataaatc attttcttgt
     5881 ccaattttac gagcagattc accgatttta cggataaacg tttctacaga cgcgtctttt
     5941 tcaaaatgaa tagtcccttc gttatcttgt gtgctggaag ggactaattc ttgtatcggt
     6001 gcgcttggtg cttggtggta gttttttgtc tcttctgaac cgattgattg tgcctgcggt
     6061 acgtcattca taggtacagt cacactctgt tcaggtgcct tttgtgggcg ttccgttttt
     6121 gttggcggtt ccgcttctgt ttcggtactt ggtttttgag gagtctgtga gctttcatca
     6181 gaaggttgct catttggttc ttctgacggt tcctcctcag gttgttgatt gtcttctggt
     6241 ttgtctggtt gattcggtgt ttctggttgt tcttcagaac ctgttggtgg agcgttcatt
     6301 tctggattat ctggtgtatc tattgtatct tcagtcggtg tttctggttg attttcttct
     6361 ccagtggaat ttgttgtcgt ttcttcagtg atcgttgcag attgcgaatt tgtcagttga
     6421 ttgttgcctt gttcattagc caaaaccgct ggcgtatacc caaatacttg acctactaat
     6481 aacaaagcag ataaacattt actcgttttt ttcacaagat tgcctccatt cttcaatcaa
     6541 tcatctagag acagctgatt gctgtctcta gtcatacttt aattgctttg tttcatttta
     6601 cgacgaacca gaacagcccc cacaccaaca aaaagaaaca gtcccaatgc aattaacaca
     6661 tcgttcactg tttctcctgt ttttggtaag ctttgttttg gtgtgctcgt cggcgtttgt
     6721 ttgacagggg ttgtcggcgt ttctttttta atgccttttg ctgtcaatgt ttgtttttca
     6781 ttcttcaaat caaagtgacg tgcttcttcc tctttatggt ctttatcctt atagacaata
     6841 tgagttgcga ccaatttctc cccgtctttt aacgtgcctg ctttgtagtc aaaggatact
     6901 ttaaactctt cagacgcttt agaaacttta cgtgcagttt ctaccgtacc aacgactgta
     6961 tcttttccat ttttatcaat cttatggaat tgagtcaccc agtatttggt ttcccctacc
     7021 gtagattctg gaaattcttg cgatacatga tcgatcaact cttggtcttt gtctggatca
     7081 aatgtttggt cgccattcac tgtaataaag cgtgtttcga tttttggttc tttcgcagtc
     7141 actgtttgat ctttattggt taaatcaaaa tgacgagcga attcttcttc ctcttctttg
     7201 tcgttataga caatgtgcgt tgcgacaatc gtgtcaccgt ctgaaaccat gtctgctttg
     7261 tagttgaacg tcacatcaaa tgtttctttt tcatcggtca cttttcgtgt ttcttttacc
     7321 gtatctaata cagtgacttt tccatcggca gtaattttat ggaattgcgt cacccaatat
     7381 ttctctgtcc caaccgcaga tttagggaat acttgggaca ctttatccac taattcttgg
     7441 tcttttttcg ggttgaaatc ttttccacct gtcgttgtca caaacaaagt agaaatttct
     7501 ggttggtctt cttctggaac cacgatttct tcgtttttca cagtaattgg ttgtacttct
     7561 ccgtctttga ccgttactaa aatcgtttct gtttgtcctg acgcttgccc ataatgctca
     7621 gggaacacaa tttccgtaaa tttatacgta ccgtctggta attttagttt ttctggttct
     7681 tcttttgttt catacacaaa ggacacattt ttattatgtg cagtcatacc ttcaatcagg
     7741 aatttcgctc ctggtaattg tagatgattg attgaatcga ctttattgaa gtctacttca
     7801 cccgttttta acaagttgtc aatcacatct aattcaaacg taagcacagg cgtcgtgtta
     7861 tcgccatagg ccacagagaa cgtggtttgt tcttcagaag gttgacggta gttgtctggc
     7921 acattttttt caatcaattg gtatttgttt tgatcgccat tgtagaattt tttgtttgcc
     7981 ttccagtgtc cgtctttgtc agtttttact gaatccaaga cttctccttt tttcacaagg
     8041 actgtttctt tatctggcgc agtaatatct gttttggcga taatatcaaa ttcgatattt
     8101 tctgcaggaa cggtatcata aacaggtttg ccttgattcc aggattcttc cgcattatag
     8161 acttctttga ctttctttcc ttctggaatg aactgttgta agtcattttt tgcagttaac
     8221 gtcacttctt tgtttggttc aatcgtaatc gtatgagttt gtttgtctag aatataccca
     8281 gctggtgctt tgacttcttt gacatccaat ttattttgaa catattttcc gtctttttca
     8341 tagaatgcag ggatttcttt taaggcgatt tcccctttgg catccgttgt atattgtttt
     8401 gtttctcccg tatcatgacg tttgatttca tacgtcacat ttggaacttt tttccctgtg
     8461 acagcgtcta gtttggttag tttgaagtcg ccatttttta gaactttaat agttagtcca
     8521 gctctattag gatctggcac ataaagcttt cctagatttt gagcaacagg atctacatag
     8581 gcaaaacttg aacctacccc tctagaattt ggatccagtg aagaaacaga aattcgtccg
     8641 tcatttgagt tttgatttgc ttttataaac aaactatttc ctgattttct tacttgaacg
     8701 ttagctgtgt tttcaacaac cgacccatcc cattgagaaa acacaccttt tgtatcctta
     8761 acttctactt cttcccctac tttaaccgtc actgtttggc tatcaaaaga aggatcttca
     8821 agtaaattgt ttatttttct atctacagtc tgtttgaaag cttcataatt agattgtcca
     8881 atagttccat aattaacaaa tgaaccacct aaaacttccc aaatatatgt ttgtgctaat
     8941 acatagttat ccattgaacc actagaatag tagccaaaat aaccaattaa agaaatcttt
     9001 tttctaatag cacttgtcgt aattaaattt tcgagtggtg tcacatcata ttgaataccc
     9061 gcactcgtga ttgcgccttc ttgaatgcaa aaaacatctt ctccgtcaat ttgccagcga
     9121 accatgtaat catgagtata cattgttcct tcaggagtaa ttcgagtcat attcacatct
     9181 ttacgaccat tttcagtcaa tgttagattg gtagcacgtg tctgtgcatt cttttccaag
     9241 attgcttgcc attcttcaga ttcttgcgcc aatcgttctt gttcagaaaa agaagtcgct
     9301 ttttgaatat cttcaatggt tataaagtat ttctcataac cttcagggac ctcttgagtc
     9361 attacattgg gcggagaaac atcttctgtc gtgtcctgcg tagttgtctc ttctgttgtt
     9421 tcacttgtgg tttcagtact gatcgcttct tcactacttt ttgattcttc agtcttcatt
     9481 acttcttcag tagaatttga ttcatttatc gtttctgcaa atgccgttac agatcctaac
     9541 atgggattga gaaatgtaac gaaaattgcc aaccaagttg ccattctttt aaacatattt
     9601 ttttcgttcc tttcatttta tttttcagtc ttctaaagta atttcttatg gacttcgaca
     9661 ccgaaacatt tttatcctcc tcaaaacaaa aaaagactag aacttataat tctagtcttc
     9721 atcaaccact tacttttctt ttatttttag tagaaatcta cagtgtgttt ttgtgacccg
     9781 tcactgtaaa taataacatc caccataaaa cctttgttgt accactgcga atttggatcg
     9841 ctaacctctg cattagccca atcataggct tcatctacag tgttaaatac ctttccacta
     9901 ttaccaatct ctccaactac aattgttggc tcttcaggtg tcgttgtgcc accatttccg
     9961 ccttgatcgc ctccaccagg attgcttggt tcttcaggcg ttgttgttcc tccacctgtg
    10021 cttgggttag aaggagttgt ttctcctcca gtatttccac cattacctgg ttgtgtaggt
    10081 gtttgagtag tgccaccatt gttttctggt gtacttggcg ttgttgtacc accagttgtt
    10141 cctgtttgat ctccagctgg tgttgttggt gtcgttgtat ttggtgtcgt ttgtgtattt
    10201 gaagtcgttc cttgtcctac tggttgattc ggatcaactt tgtctttctc ttgaatcact
    10261 gtttgagcca cagtttcacc tgcgctgttt tgtacacttg ctacattgcc attaaatact
    10321 aatttatttc ctgcatagat taaatccaca tttgtaatct tattcatgtc cgctaatttt
    10381 tggacagtaa tatttgttgc ttgactgatt gcgcttaggg tatctcccca gacaattgtg
    10441 tattcattcc ctttaatttc tgctttgatt tgttccgccg aacgaggtgt ccaaactttg
    10501 ttttctgctg cttcagcatt cgttcccaca gcaagactaa ctcctccgac tacgattcct
    10561 agtgccaata ctttcaacat tttcattgtt tttcgcctcc gatttgaata aatttattta
    10621 caaaaacata gtaccaaact tatgtctatc agtcgatata ttttttgtag atttttttat
    10681 tttttgatat actaactttg gtactaaaat aactaaaagg aatgtcacac atgcaactta
    10741 ataatttaaa tgaaatacat gattgggctc aaaataattt acttaatcgt aaacaagctg
    10801 ccaaaatcac tggacaaaac tatgatgctt tttctagctc tttacgtaga aataatatca
    10861 aaccatttct agaaataggc gacgaacctg gtccttccat cattcgctta tatctaagag
    10921 aagatattga aaaatacgca aaacagctcg aagaaaaaag acagaaacgt caatgaggcg
    10981 ttttctgtct tggatttaca agtaaaacaa taataaacac ttttacaacg gaggttctat
    11041 gaataagaaa tacattgttt cagttggttc aatcgtactg attgtactcg ctattattgc
    11101 tttttctgta tccaacaaaa cagaaacaca gccacaaaat acggttcaat cgactactac
    11161 aagtcaatct agcgatcatt ccgtctatcc ttcactaaat agtttaaaag aaacgcatgg
    11221 acaagattta attgatcttg gttcttctca aattactact gtcttatttg aaaagaaaaa
    11281 tattgcgttt attactccta aagaaggaac tgaccttgaa ttttctatca aagtaacaaa
    11341 taccaaagaa aatttattct ccattcctga cattcaatat acccctaaca agttaaagat
    11401 tggggatacg tttggtttgg ctaaagtaaa tgaacaatac tttgcttact cactgaaata
    11461 agtttctaat agcaagagga aaaaccctct tgttattttt atcacataat cattttataa
    11521 aaacgcttcg aaaatatcaa catttttgaa gaaactattt ttctaatttc tcaatttctt
    11581 ccaagggatt ttcgctaatg acccatacag gcacagattg ctctcgaggc tccccttctt
    11641 ctaaccacca acctgcataa tgaacttctt cctcctcagc attaccatga taccaaccaa
    11701 caaaggattc atctacaacg aatgaaattt cttcttctgg atcaaataat tcatttttcc
    11761 atgcttcaat tgcttgttct ttggtgtact tttgtttgtt aaatacacca caaatttcgt
    11821 catctacata aaaaatttca aaatcaaaat tactcgttcc ttttactggc atcttaactt
    11881 ttcctccttt gactcacttc aatagatata ccactcatgt tcattccatt gtttactagt
    11941 taattggagt tttatcttag ggctttgtgc actctttcca aaaacttgag cgtcgttttt
    12001 ttcttctaac caaaaaaggt aatttcttac attcggtact aaatgttttt cctgcatttc
    12061 ttcatgaaaa acatataatt ctgcttcatt tttcaaccaa ttcattattc attccctcat
    12121 tcacttcttc ataccgccat tcagatagtg agatttcttt ttgtttctcc acttgtccta
    12181 taaaaatcgc tgtattcaac aagctgatca cacttaataa taaagcaatg aaactaatgg
    12241 ttacacttac tctttcacat tttgtcattt tgtttccttc tttactcgtt tgttatttct
    12301 tgtaataggt ctaataactc cttcaccgaa atttgcaaat ggttcgcaaa aatttcaata
    12361 aaagcactcg cacacattac cagaccttca actgatatat cactgcgaca ataaaattct
    12421 ggagaaacac tgatttctga ttctttctta caattgaacg aaaaaactat ttttgaatca
    12481 aattccggta attcttccac aactatgttt tgggattcta ccaagaaagg gagtgcctgt
    12541 tctaatgctt cttctggtgt ttgagcagat agaataattg cttcttgttc ttttatttca
    12601 ttgttctcaa atactttata actttttttc attgttagtt cctctttcat tcctctctca
    12661 attagctaag ccgttactgg tacgccattc tctaaattta gtatcgctaa tccttaatgc
    12721 tcgtcgaata tcaatcattc gataacctat tgcacgaagt tctttatatc ttttagcagt
    12781 tagcgtctct aacgtcattt caggcaatgg gttcgaatca aaaatagaat tcgaaccgat
    12841 tatttgggcc aattgttgta cttgtaacag ctcttctgaa cttaattcag tgattgctcg
    12901 attgcctatc attgcataca ttttctgtcg aatatacttc ttttcttgtt tagacaacat
    12961 ttctatccac tcctattttc attatcattt gttacctcca cttcagtaaa tccacaaata
    13021 aatgaattca tattccaagt accattccct tcaactacaa cttttctcct gtcctctttc
    13081 ggatattcaa gaatcagacc gtttaccaat atgtttttca ctactactaa tttgtttgta
    13141 taccgtggaa ttgctgttcc gatatacttc actttatttc ctacgctaat tgtcatttcc
    13201 tcactcctat tcttttttat ccaaaacttt ttcctgtcct attcctgcca acgaatatac
    13261 ttcactgcat gcttaaacaa aacaaaatgt tgccctgttt tatttccgtc ttttacttgt
    13321 tccacataca gttcatattt ggtcacacct tttaaaacgc cagatacttt ttttcatcca
    13381 ttgtcacaat tagttttttc atcaatcgat tgatactgca ctgctcattt ttcttgttca
    13441 tatagtattc ctttcttttt caaaactagc ctcttttgtt tactagtttt catcaatcat
    13501 caacgattta acagattttt tctttcgcag ggtttctagc gtcagcaacc ccaaactcta
    13561 aaaaagtgcc tttgctccct tgaaaaacga gcggacgatt gtactcgtca tcatatgttt
    13621 ttcatactac ctgatccacg caaataacct ttttttgttc tgtgccacac ccatccatac
    13681 gtatcgcttg agattcacca tactttttta ggtggtttat ccttcctaag tccctgttag
    13741 acacgctgat taacttagaa agtcctgttt gtatccaatt ttcaaagatc tcccaaaaga
    13801 aataagtagg tttacgtgta tcgcttctgt tcaaagtacc tgtttgtact tgagtatttc
    13861 tactcccctc gttcaaggga ttccttccac cagctagctt ttcctttcat catcaaatgt
    13921 tcctgaaaag catttacgtt tcgtgtccgt gtgcttactt attctttttt cgatagcgat
    13981 tatccgctcg ctactcgcta ttttatactt tgtttgttag ctcaaataaa aactaacaaa
    14041 caaaaaaggc tagtttcttt ccgttttatt tttttaactg tcgataaata gttgttagtt
    14101 taattccaag ttctaaaacc aatacgccca acaaaaccac aggaaaaaca attggggcaa
    14161 acaagtaaaa tcggatagtt ttcatgtctt ccctctttct ttttggtgtc gctgtggtcg
    14221 ttcttgccgt tcaatacttc gatacatttt ttattcatta tttgctcgtt ttgttttcct
    14281 ctggactgct tttcttgctt tttgaatact ttatacgctt ttcgtgcctg aattaatagg
    14341 tcttgtcggc gtttcattcg tgagaaaaca atatttgaaa ccatcaaatc ctggttcgaa
    14401 caaccacgat tcgcaataat tttcccatca aaaaaaatag ttttcacttg agaaaaaaac
    14461 agttggaaat cttgttttgt tgctaatttt tttgtcaaaa tcactgtgct tttccctgtt
    14521 gaatctacaa tgattcttcg aacaatccga ttcgataact tccaacgttg ccgattaaac
    14581 aatgttactt cacaacaaat atgacgtaag tgattagggg tcattttcat gtgtccaatt
    14641 cctccaacca attatgcatc gaaattttgg gcaattgtga cagcccataa atctgcgttt
    14701 ccaggtcttc ataactacaa gaacgcttgg cttgaagtaa tgaatattct gcttgatctg
    14761 atagttttgt tgccattgga tctagattca attctcttac acaacattcc caaaaattca
    14821 atagcgctct aaaataaaat ccttttcgat tctgaattgc ttttccttca tgacgagcaa
    14881 ttttttcatt ttgaatacac cgttgatacg ttttgcttaa atcttctgca aatgtttccc
    14941 ctaaaattcg atactcgctt ccatcagata aaccatgtgt ttgattatac tttcgttgcc
    15001 gcttttctat aattcgttta ctgtcgtata tcatgtttag ctgctctttc gcttcacgcc
    15061 attcagctga actgcgaaca atccatgtca aaacatcttt cggaacaaac gtactttcgc
    15121 tgtaatgagc aataaactct ttttgccaat catcagccat ttcaacagga ctagtcgtat
    15181 cgatttttgt atctagattt tctgtatcta gattgttaga agaattatta aataattgtg
    15241 cagaattttc acatggattt ttttcgtcaa aatcatcgaa aatcgctaaa ttatcaacag
    15301 attcctcctc ttcttttcct tgtgcagaat ctacactggt tgagtcaaca ggacctaatg
    15361 aatacacatc attttctgtc gtatctaatt ctcctagata caaccgagct ttgtttcttt
    15421 ttcctgtttg ttgatcaaat cccatatcct ctatttctag tagctcatat tctttcaatt
    15481 cttttttgat tgtattaaca gaacgtctgc aacaattcaa tgcattggct aattcttgtt
    15541 ccgtaaattc aaaataaaca aaaccttgac tatctacttg atttcgttga atggctaaat
    15601 ctaagcgtga ccgaaaaacc atatacgcga tttttgcttt atcagacaat gagcgatatt
    15661 tttcacttgt aataaatacc tttggaaatt gatagtataa ttcattgtat ttacgagttg
    15721 cggtaaatct ttttgttgcc atcgttatta cacctcatct tgtccatata ttaagaaaga
    15781 attttggtgt tggtagacac gaacatcttc cattgtagca atcaattctt ttagataaat
    15841 tcgaccgaat tcaattgtta gtaagtcgta atcttctaat tcatttttga tttcttcaat
    15901 ttcttcttca gacacattga gtaaaatggc taattcttga ttggtataag aaatatagac
    15961 atgtttcttt tcatcaatcc gtttttcaat taaggattct tctaagtact cattcagtaa
    16021 gctataggcg tattttgctt gtgcagtcat cgcataatat tttgacgatt ttataaagaa
    16081 tagttttggc atatgaaaag ttgcttgtgt cagcacatct gttgcgcgaa atttgttttt
    16141 taccatattt tttcctccta gaaaaacacg caaaaaaagc tctgtataaa tcgaattaca
    16201 cagagtttct tcttgttgat atttaatttc taaaatacgt ttcgggtaaa aactaaacac
    16261 ttactaatat ttagattgta gaatcttcgc cttcatttat tcattttaaa gagaaaaaga
    16321 tcaagtgaaa tgtgcccaaa agtttgattc tattaatttt taaacaccat tcataatata
    16381 tttcctataa tgtaaaaagc gtcgatactt attaaagcat cgacgttatt tttatagtaa
    16441 atttatcaaa atttttgcca acattttttc cgattttttt ccctatttct atctttcctt
    16501 atctttctta gttaatatta tttgatgtta atatatcact actaatatta aaaaacattg
    16561 atttatcata ggataaagag tgatagttta atacttaaag agacatatta attttattta
    16621 aaaattgaca ttaatgactc ttaatcagcg ggtcgcgggt tcgagcccct cacggcccat
    16681 tgggtgccaa acccacggaa caggtaaatg ttggtgcgaa agcatttcga aaacatttac
    16741 gctgttcttt ttttattttt caaaataact agggcttttt atttttaact caaagtccac
    16801 tgtagccctt tggactttga gttaaaaaag aagcataatt atgaacagta aagtttctaa
    16861 tataacaata attcgagaag ctttgcaaaa tcatgctgcc aatgaactct ctaaacatat
    16921 aggattaaat ctaagtacta taaaaggtta aaaagtgaag aatatttaat agaaaaacta
    16981 aatttacttg ctgctttccc cttttccatt aatacaacaa tcagagagtg tctttttttc
    17041 gaaaaaatta gaataaataa aagacacgta gtttttttat ctacatgtct tccatcgact
    17101 acaagttatc atttctcaaa acgtatttag cttttaattg attaatcgtt tcacgttgtc
    17161 tacacattgc ctaattaaga ttttcttatt agaagcatca atcccgcaat aatacctaaa
    17221 actccaggaa ttacccacaa taatgttaaa agaaaaaatg gaataataga taaaatgagt
    17281 aaactaatac catagaccac tgttttttga ttttttctta ctaaaaaacc tattatgaaa
    17341 atcattatcg aaaaaactag ggtaattcct ccaaataaaa caacattccc gcttagattg
    17401 actaacatat ctatatcctt actggttagt tctgtagtat ttaaagtagt tgaatcaata
    17461 gaagatagaa acataccacc catcaatgct aaaagactac ataaaatccc tgttattcca
    17521 ctaattaaaa tcaaaataaa ctctgttttt ctcttattca tttaattctc tccagattaa
    17581 gtgttttaga attagaaatt ttttgaccat agttaatagc ttctaatgaa cgaataacaa
    17641 ccactgacgt gttagaaaaa taatccccaa tatgttgttt cttgggcgtg aaagcagcca
    17701 ccaaataccc aatttgaaat acaaaatttt ttaatataaa cctacatata cactcacgaa
    17761 taaagatagt ttgccaactt agctcttttt cctcaaagct cactactctt atcccaaaaa
    17821 tcattttccc aattgtttgt cctgaattca gcttagtcaa aagtacaaaa taggttaaat
    17881 aaatagctaa tgacgctata ccataaaaag taataatgga tttgtcagat tccagtccca
    17941 tgattttgta aaagagacct atagtagcat gggtgatgat gctaatacaa attaaatcaa
    18001 taagaaaagc aaaaaatcgt aaccaaaatc ccgcaaatac ataattaggc aaagtttcaa
    18061 tgccactctt cttattcatt ttatagccct ccatagtaat acattggttt tggagtatca
    18121 gaaaatccaa gtttttcaaa aactgaaatt acacgatctg atgtagttgc ctttagccct
    18181 tgccattcag caatcttatt acctaaccaa gaagaagcaa agccagttgt gtcattttta
    18241 tattctataa ccgtcgcatc agttaatttc ttctcttttt ttagggaatc taatgcatct
    18301 tcagagtaac caatttgatc caccaaccca ttttctactg cttggttacc atcataaatt
    18361 cttccatccg caagttcttt cactgcatta gtatccatat ttcttccttt tgctacaaca
    18421 ttgacaaaac gatcataagc actcatcaca aattcctgta taactttatt ctcttcctct
    18481 gaaggtttgt gttgaggtga catcatatct tttaatgcac cacttttata cgtcgcatca
    18541 gttacgccaa gtttttctaa taagccagaa taatttaaac cagaaataat gacaccaatt
    18601 gatccagttg ttgtttcttc cgtagcaaat attttatctg aaccagcaga aatgtagtac
    18661 cctccacttg ccgctgtatt tttaaatgca gtatagatag gaatatctag ctttttaatt
    18721 tttgccattt ctttagcaat ctcagcactt tcatatattc ctcccccagg tgaatttact
    18781 tctaataaca ctcccttaac cgctttgtct tcttgaattt tctttagctg tgttaaaaaa
    18841 ttctgatggt tatattgttc cctactaaaa agattgcttt cacctgtatc tgcaataact
    18901 ccattgacgg atagtttgac aatcttttta tttgatgcgc cttcttctaa cgtttcttct
    18961 gtgatttcgt tagatccgta aaaaatctta tttattcctg ttagggttgt atttgatgct
    19021 tcctctttat cttttcctgg aattagcgcg acgataatgg atagtaacaa taatccgcac
    19081 gctactccca cagcaatcca tcttctttta ttcatataaa ctcctcctat attcttagta
    19141 aaaataatta ttttttattt agaaacacat cgacaatttg ttgccaatat gttttttctc
    19201 ctacaattaa agacaactct ccagttaatc cataacgaaa cggaaacgat tgagttgttt
    19261 ttaacgttcc tttcactgca taaaaaacac ctttctcagt ggcagtagaa ttttctgaaa
    19321 tttcatctaa agttccgtcg atcgtaatcg gagccccacc tttttgatcc aatttaaaat
    19381 gaaccttcat cccttcttta atataggtac tttcattcgc aggtaaaagt gctgtaaatt
    19441 tcatttttga tttttcgtgg atcggataaa tttctgcaag cactgttcct ttggctagtt
    19501 ctgcttgtcc ctttgtttct tcatttaaat gaaccgttcc agatacagga gcttttagta
    19561 ttccttttga aagttgatcg tccaaacttt tgataactga ctggttcttc tcttgttttt
    19621 catttagctc tgtaactgtt tgttttacag ttgttattgc ttgttctagc tcttgctttc
    19681 ctttttccgt ttgactaatt ctgtcattct cagaagtagt tggtttaact aatttagctt
    19741 tttctgactg atactgttct acttcttttt ttagttgctc aatttgttca tttatgcttg
    19801 ctgaaattgt cagtttcact tgattttttt gatcatcaga aacattattt agttgttctt
    19861 gccaattctc atagtttgcc atcatttctt ttgaaaaatc ttttaatgat tgattattac
    19921 tccaagctac ctgtacttgt tgccattctt gtaatttact atctgaactc tcaatttgtt
    19981 tttctagttg tctttttgtc ttttcgtata cttctaattg cttttgatca tttaattcac
    20041 tttgcttcaa agcgtagcgg agtgattctt tttctgacaa catgctcttt aattgattac
    20101 tgtagccaaa actgtcatcc gttgaaaaca aattttttcc tttacttata ctttcaataa
    20161 atagctgagc catctttatt tgttgagtaa tacgttggtt ttcttgttct atctgagcct
    20221 tttcattctc tattaaagaa caatccaatc taaccaacac atcgtcctta tgaacaaatt
    20281 gattttcttt taggaagttt tccattattt tagcttctaa tggcacttgt aacttctcta
    20341 ttttttcggg gttcaattga gccgttgtcc gaataacaac ctctttttta gcaaaggtta
    20401 aaaacaatcc aataatacat aaaaaaagaa ttacagggta taacacccaa cggtagaagc
    20461 tatgatgttg tttagtatag atactagaat gttcgaacca attattattc tgcataaatt
    20521 gtttccctct cctagatttc ccataatttt tgatagatac catcggcaaa acgaagttca
    20581 tcgtgcgttc ctttttctac caaatgccct tcatggagaa ctaaaatttg atcacatgct
    20641 tttgctattg gtaaatgatg cgcaataaaa ataatcgttt tttcttttaa attgaggaga
    20701 taattcataa ttgcatgttc taacaaggta tctaatccac tagttgcttc atctaaaatt
    20761 aatatgtccg gattttttaa taaagcccgt gctaaggcta atcgttgtcg ttgccctcct
    20821 gaaagatt
//
LOCUS       DACEEZ010000046        20820 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20313_2017
            SAMN12639966-rid9879223.denovo.046, whole genome shotgun sequence.
ACCESSION   DACEEZ010000046 DACEEZ010000000
VERSION     DACEEZ010000046.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639966
            Sequence Read Archive: SRR10023158
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 20820)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20820)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741683.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741683.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:44:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,016
            CDSs (total)                      :: 2,962
            Genes (coding)                    :: 2,889
            CDSs (with protein)               :: 2,889
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 36 of 73
            Pseudo Genes (incomplete)         :: 29 of 73
            Pseudo Genes (internal stop)      :: 18 of 73
            Pseudo Genes (multiple problems)  :: 8 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20820
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639966-rid9879223.denovo.046"
                     /isolate="E20313_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            <1..319
                     /locus_tag="IWO89_001706"
     CDS             <1..319
                     /locus_tag="IWO89_001706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HAQ0456453.1"
                     /translation="DGKLAIDGLAPGAYQLVETQAPTGYELDATPVTFKIEKEQEVAI
                     FLTKENRKVADSSDIRGNNPTSSGNKHLPKTGETIISQFILSILGVLLVFISIKFRKK
                     RNI"
     gene            complement(743..913)
                     /locus_tag="IWO89_001707"
     CDS             complement(743..913)
                     /locus_tag="IWO89_001707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016615560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HAQ0456454.1"
                     /translation="MPRLTNYFLSCTNPKEIEFLVTDMNATYFQLTKHVLPNAKLVID
                     RLLGFSTPFKEA"
     gene            complement(1107..1286)
                     /locus_tag="IWO89_001708"
     CDS             complement(1107..1286)
                     /locus_tag="IWO89_001708"
                     /inference="COORDINATES: protein motif:HMM:NF026041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0456455.1"
                     /translation="MPMVIRLKKQRYTCKNCRSHWNAQSYFIRPRHSISNHVRHKITS
                     LLTEKVSLFFISKSC"
     gene            1673..2785
                     /locus_tag="IWO89_001709"
     CDS             1673..2785
                     /locus_tag="IWO89_001709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HAQ0456456.1"
                     /translation="MTVKNKSKKKGRQQVDFYQSLQLDPFILKQKIREAASKKEKCMY
                     ICSLFLRSLFIVLFAICFIIIITTLFESKHKPYAVVLFCMLMSIRFVDFGYKISHSII
                     GLAIVMFSLLVAPYVQLIKWSVMGMLIHFILLSSILMATASDPKMGNASLYGFSYLFI
                     VYSLPKDSLNKNFFTQTSSLLFLFFCWFSVLLYRKHREKNKDKSLFKEIFLKGMYSQE
                     KIWMLIYAFGISLLIVAGEYVPFQRLMWAGFAFSSIVSSYGLMSIGFKERAVDRIIGS
                     LIGCVLFIGISQFIPFNWVGILGGLALGVCSTYRYKTVFNSFGALAITASLFGVPGAV
                     TIRIFENILGVCLGIMYIGVTEILIRKIREKHGLNH"
     gene            complement(2863..3156)
                     /locus_tag="IWO89_001710"
     CDS             complement(2863..3156)
                     /locus_tag="IWO89_001710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antibiotic biosynthesis monooxygenase"
                     /protein_id="HAQ0456457.1"
                     /translation="MSITVNILYTSTNGQARKFAEEMVERNIVKAIRAEEGNEKYDYY
                     FPLEDPESLLLIDRWKDEEAIEKHHKSEMMAQINELRKKYKLKMKVERFTDLP"
     gene            3324..3638
                     /locus_tag="IWO89_001711"
     CDS             3324..3638
                     /locus_tag="IWO89_001711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIB"
                     /protein_id="HAQ0456458.1"
                     /translation="MKKALIICAAGMSSSMMASKTTDFFKQKREEVLVDAVSATEGDN
                     MIKNSDFDLFLISPQTTMFLDKFKKLGESVGKPVVSIPFQAYVPIPTGIQKLAEVIEE
                     NI"
     gene            3659..5638
                     /locus_tag="IWO89_001712"
     CDS             3659..5638
                     /locus_tag="IWO89_001712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator"
                     /protein_id="HAQ0456459.1"
                     /translation="MKQKEKLLLRYLIDHKKEYVTSQRLASELLLSDRTIRNYLQRIK
                     ELVEKNGGKIIAKPGYGYQLHILQRLTFDLFLSRQEIVPGYAREPQGFYESEDRQKYI
                     LNQLLLEDAILFMDDLAEELYISRSSLTKDMQEIKERLIPYSLKIVSKHGQGTWIEGE
                     ERNRRHFIMDTFFGNHYGNSLKEYLGQSRFFPDISFEELVIIILDETREAKLKVSDFI
                     IQNLTLHLALGIKRLREGFEIKELGIEQEVSKRVEYQVAQRIVQRIEVIAGVSFPEEE
                     IAYLTLHLMAKSNHKENQNDQELSKELAKVLKRLSQEFHFSLVEDLQLFNGLLDHLSP
                     MMIRLSRGIRIENPLTEEIKKENPKVFDAVKRHFSNMPALKNYTINEDEWAYLALHLM
                     AALEKERAAHKLHALIICATGYGSAQLLKNRVVSEFGKNITVVSVKGYYEINETTLKG
                     IDLIISSIDLSTMFFKIPVLHVSIFLNEDDVRKIRKVVGESIPYHSSDRPIPAFSLQQ
                     KKQIYTEQLSEKFFKTYTYEPKKEEILHDLLQKLSINEEEGYIREMRKQIQQRENMGQ
                     IIFSDTVAVPHPAVPVGIETKVAVALIPSGMQWDQYQEIHFVFLVSPSYVENEGITVV
                     TKAIVRLVDQLKVQQEILAEPIFENFSSKFIKLIE"
     gene            5651..5977
                     /locus_tag="IWO89_001713"
     CDS             5651..5977
                     /locus_tag="IWO89_001713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIA"
                     /protein_id="HAQ0456460.1"
                     /translation="MAEKINSETLQVTSFDIIFHSGNARTLIHEAFRSMRKEMFEEVE
                     EKLRQANEEILHAHQSQTQLLKEYASGTKIEMEIIMVHAQDHLMTTMTLLEVAQEMNY
                     LYQKNK"
     gene            5993..6511
                     /locus_tag="IWO89_001714"
     CDS             5993..6511
                     /locus_tag="IWO89_001714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0456461.1"
                     /translation="MQDTVKRKIELEKKQLSLKNMYFNRYLIVRYLTAFFFFMNMQWM
                     ILLLSAKSLGSSLPMILILAILPAVGEQVKLYRKHQTNVPWTKWYFLFQSVFNLLVIP
                     VLFTSGFTFLYPFMANNNGGQLFVFILVVSGIFASAFIQYRLKKISLNQDQQYIRIKQ
                     YEKALYLGKENN"
     gene            6511..7863
                     /gene="celB"
                     /locus_tag="IWO89_001715"
     CDS             6511..7863
                     /gene="celB"
                     /locus_tag="IWO89_001715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIC"
                     /protein_id="HAQ0456462.1"
                     /translation="MFDFLQKYLMGPMGKIAQLKIVRAVMAAGMASIPFTIVGSMFLV
                     LNILPLPFPFLEGFFNATFFKVSDLYMIVNTMTMGILSIYFAIVFAYELTSIERDEQK
                     LNVNPLTGALLSVFAFFMCIPELIMTDGKISLIASINETETVVNGIRMGSFVERLGTS
                     GIFTAIIMSIIAVELYCMCVKRNWVIKMPDVVPPGVSRSFTALIPTFVIAFVVMIING
                     LLVALGTDIFKMIAIPFGFVTQLTNTWLGVMVIYFLIHALWIVGIHGANIITSFLTPI
                     VLANMAANAQGANYPLAGEFNNSYVTVGGSGATLGLIIFIAFLAKSDQLKVLGKASLV
                     PGLFNINEPIIFGMPIVYNPYLAVPFFLAPMASATLAYFAIRLEIVKPMLAQMPWPSP
                     VGIGAFVGSGGDWKAAVLAVICAIIAFIIWFPFIKFYDNKLLNEEREKAAELASEKLK
                     "
     gene            complement(7947..9452)
                     /locus_tag="IWO89_001716"
     CDS             complement(7947..9452)
                     /locus_tag="IWO89_001716"
                     /EC_number="6.3.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--L-
                     lysine ligase"
                     /protein_id="HAQ0456463.1"
                     /translation="MSFSLEDIRHLLLKENLLKEFVSTRGWHLDVPEKAEFLQLSYDS
                     RKADASTLFFCKGMNFKEEYLDSAIEQGIQYYVSEQPYENSAVGIIVTDIRKAMALLA
                     MAFYDYPQNKLKVIGFTGTKGKTTAAYFTKAILDHTTNKKTALLSTMNTTLDGKTYFK
                     SHLTTPESLDLYRMMSEAVENGMTHLVMEVSSQAYKTQRVYGLTLDVGIFLNISPDHI
                     SPIEHPTFDDYFYCKRQLILNSKTVVLNHESDYFDLLRETADLFQIPTITYGRSEESN
                     YQVIRSDKGTHGFTLSSHEDRLAICDTAYDILLAGGFNQENAASAIIAAALTGASSQD
                     AQDGLKEARVPGRMDQLIQSNGAHVYVDYAHNYLSLKTLLKFAKNEHPDGRVIVVLGS
                     PGNKAISRRHDFGKVLSETADIAFLTADDPAFEDPKKIAEEINEAITNPDLIVHYEMD
                     RPEAIRRALAESAPQDSVVIAGKGVDPYQKINGVDEPYEGDYAIVKRLIEE"
     gene            complement(9544..10134)
                     /locus_tag="IWO89_001717"
     CDS             complement(9544..10134)
                     /locus_tag="IWO89_001717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HAQ0456464.1"
                     /translation="MTQEIKKLPVIFLGLFFITVGLNWFLLPHDIASAGVGAIGHLVE
                     TAFSIQRSVTVWSINLMMLLLAAVLLGKSVFFKTVAGSLLFPVVLEIVPKVEMISSHF
                     FSLISGSLLFSLGVYTLYTVGASNGGITIPPIIFHKFFRLPMPQGLLLTNSLIVFLNY
                     YVFGLLETLFVLLSITVSSLFMKILIRLSPVSRKFE"
     gene            10313..11959
                     /locus_tag="IWO89_001718"
     CDS             10313..11959
                     /locus_tag="IWO89_001718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HAQ0456465.1"
                     /translation="MDNQRKPALDQLTPQEKMARGSAWMTASNIISRLLGAVYIIPWY
                     AWMGENAKAANGLFNMGYNIYALFLLISTAGIPAAIAKQTARYNSLNEYGTSRRLFIR
                     ALQMMGGLGLLFALFMYIASPWLAHASGGGEELIPTMRSLSIAVLVFPCMSVIRGYLQ
                     GNQEMMPYALSQIVEQVARIFYMLLATFIIMKVMDGDYVTAVTQSTFAAFIGMLASIL
                     VLLYFLKKHQAYTSAFIHYSENKINIATKELLLDTVKEAIPFIIVGSGVTIFKLVDQF
                     TFMKIMSASTEYSNAQLLDLFSIFSANPDKLTMVVIALATSIASTGLPLITEAVTLKD
                     RVGLAKLTSGNLQLFSFFMFPATFGVMLLAYPLNTLFYTPDSLGSNVLIQASFVGLFL
                     GLYMLVSNMLQGMFENKAAINYLVIGFLVKLLLQYPAIRLFEVYGPLLATMIGFAVSC
                     TLILKRIHEVARFKPSFVWRRTLLIFILTLIMLLASWITKMIFGAFLNEDSKFQSLLL
                     ILVVAGIGALVYGYLALKIRLAERLLGPGIARLRHKLKIK"
     gene            12008..12727
                     /locus_tag="IWO89_001719"
     CDS             12008..12727
                     /locus_tag="IWO89_001719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA pseudouridine synthase"
                     /protein_id="HAQ0456466.1"
                     /translation="MRLDKFLADMGLGSRKEVKGLIKKGTIEVNGQVVKSDKAQVNEF
                     EDAVTYLGEIITYQKDFYYILHKPAGVISATQDNHDQTVIDLLEDQDYREDLFPVGRL
                     DKDTEGLLILTNDGVFAHQLLSPKKHVEKEYLAEVSGIMTEEDIRLFAEGLIIDGDEQ
                     TLPAKLQIDQVDQETETSIIRLILHEGKFHQVKRMVKAVGKEVLYLKRIRMGKFWLPK
                     DLNKGQYRPMTKEELEQVREK"
     gene            13206..14153
                     /locus_tag="IWO89_001720"
     CDS             13206..14153
                     /locus_tag="IWO89_001720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAQ0456467.1"
                     /translation="MKKRKGFNHFFMNLWRYKALVLMAVPGMLWMIFFFYIPVMANVV
                     AFKNFHISADGFLASLRESPWVGWENFKFLFSSDQAFLITKNTILYNVAFILLNLLIS
                     VVFAIIMSELRNKRLVKVYQTMSLLPYFLSWVIISYFVYAFLSPDKGIFNQWITGHGG
                     EAVNWYNEPKYWPFILVFIGTWKGIGYNSIIYFASVMGIDPTYYEAAMVDGASKWQQI
                     KHVTIPQLIPLMTILTILAVGNIFRADFGLFYNIPRNSGALYDVTQVLDTYIYNGLTS
                     TGDFGMTAAAGLYQSVVGFVLLMITNTIARRFDSDSALF"
     gene            14165..15085
                     /locus_tag="IWO89_001721"
     CDS             14165..15085
                     /locus_tag="IWO89_001721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HAQ0456468.1"
                     /translation="MKKKKVERVQVRSFNKTANFFSNIMIALFAISCILPFIFVIAIS
                     FTDESILSTHGYSFWPEKFSIFGYTYLFDQMQDKIFQALFVTVLVTVLGTLINSTATS
                     LYAYAISRSNFPFRRFFTVFCLITMLFSPGMVANYLVMTNLLQLKDTIWALILPMAVS
                     PFNIIVMRTFFKRSVSDSIIESARIDGASELRIFVQIVLPLAVPGIATISLFAALGYW
                     NDWFNALLYIQDDKLVPLQYLLMKIQSNIQYLTQNSGAGSQLTGGLASVPGESARMAI
                     VVISTLPIAISYPFFQKHFVKGLTIGGVKE"
     gene            15126..16583
                     /locus_tag="IWO89_001722"
     CDS             15126..16583
                     /locus_tag="IWO89_001722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HAQ0456469.1"
                     /translation="MKLWKKLAFTGVSALLLGTLAACGGSGSKDQAEASNSDTLQMYQ
                     IGDKPDNFDQLMEVANKRIEKEIGVKVNINYIGWGDYEKKMNVIISSGENYDIAFANN
                     YVSNAQKGAFADLTELAPKYAEKAYNDLDEAYIKGNLVNGKLYAFPVNGNVFAQQVLT
                     FNKALLDKYDLSIDGIQSYADAEKVLKEFHEKEPNTAAFAIGQGFKVQGNFDYPLGNT
                     LPFAIDLDGDDSKIINQYDNETYIGLLRTMHSWYKQGLIPSDAATSNKDYPLEGNTWL
                     MRGETQGPYDYGDTILTNAAKQELVSKAITVPLKSTAQAQMANFVVSNTSKNKEKSVE
                     LLGLLNSDPELLNGLVWGIEGEAWEKVDGSDDKIKLLDGYQPNTHMSAWNTGNNKILY
                     TQESITDDMIAKRDQSIADAETSPILGFSFNTDSVKTELSNISNVMNQYLDGLNTGTV
                     DPDETLPKLKDALDKAGYDKVLKEMQKQYDEFRQE"
     gene            16928..17542
                     /locus_tag="IWO89_001723"
     CDS             16928..17542
                     /locus_tag="IWO89_001723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF624 domain-containing protein"
                     /protein_id="HAQ0456470.1"
                     /translation="MLGKVLEELFIRIWVIIKLTLYFWIYTFAGGIIFGLGAAWKTVN
                     ELFYLYGFEYKEITIKRGWNIYKRNFLRGNLLFSLFLSGTALLSYNLYLSVQIKGLIF
                     LAIDFILLFALLCLFATYQYSMILDSEYTISVPNLLKLSFISVFSSFSSFLKIIIGSG
                     IILGVTWQFKGLILFGVIGLLTVWNGTMTTHWREELDKQLESYE"
     gene            17535..19259
                     /locus_tag="IWO89_001724"
     CDS             17535..19259
                     /locus_tag="IWO89_001724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HAQ0456471.1"
                     /translation="MNKFRILFSKGTLLNRLLRKYAVIMIGVTMTATVIFSIHTWEQN
                     QKQAENMTSDAVQSTSRMLNDKTTLSRIIKNQLVGNSEKIENVTTYLTKPIDQYLMYV
                     YEQQNSTDELVSFPNQIKDLYANYEELSAIYIVLNQLPEYYESSRENKGGEIKKGTPK
                     LLDAFYIKLPITQGGAESGSIFIGFQKKELDMILENLTSFNGLSLYMISGTTNRLYTF
                     HDNKIAKETFERQEMIITESLKKNSQLPIDDLEKNNFVQHQELSDDYRILAVLDKKSV
                     KIETGKNLAPLIIGIIILDNILLIFLYKTFKRYSQQVSIVMQAMDQTAAGNLETRIDI
                     TNTEYELRELSIGINEMLDSINQFVEDIYKLEIKQQDAHMRALQAQINPHFLYNTLEY
                     IRMYAISEGSEELADVVYAFSALLRNNTNQEKTITLKEELDFCEKYVYLYQMRYPNRV
                     AYHFMIDPDLEKIEVPKFVIQPLVENYFKHGIDFTRFDNALSVKVLQEGKRVRIIIKD
                     NGKGMTEKRLKQIEEKLSHPKVELHGSIGLQNVNERLRASFGSSYYMSLENNETGGLT
                     VSITFKEG"
     gene            19256..20707
                     /locus_tag="IWO89_001725"
     CDS             19256..20707
                     /locus_tag="IWO89_001725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAQ0456472.1"
                     /translation="MSVYQVLLVDDEYMILNGLKKIIDWQSLGFQIVATAENAKEGLA
                     VLEQRQIDLVVTDVTMPEINGLEFIEAAQKERHNFEFMILSGYQEFDYLKGGMQLGAV
                     NYLMKPVNKFELVESLKKIKTRLDQQNEQKNQQEIYQELLFSQWLNEELDEISEEEII
                     GQISEKQHRIVLIQLARTHDTLINRWLQERQQIFYYQRNYGDRVLITLLLEADEVDGF
                     CCFVQENFPDQEWLISIGEETDSLDKIPETFQQAKDNLQLHQFYGDKDEHVFYAVQAE
                     TKDQTIDFSSFRRVLQNKQLEVAQKMITDFFEQFQLAAMMPEDIRYSSFLLFMEIQRE
                     LIDLEDEEYLQGIEKIHQAKTVQELQQLLLSFIKKHQRQKKYSDNVEKVIVILHQHYQ
                     EPLTLKEVSENLHLNVMYLGQLFKKETKKSFSAYLNHLRMEKAKQLLLHSNQNINEIA
                     SEIGYNNTTYFSKLFKKIVGRSPKEYRENSGES"
BASE COUNT         6716 a         3512 c         4173 g         6419 t
ORIGIN      
        1 agatggaaaa ctggcgatag atggcttagc accaggagca tatcagctgg tagaaactca
       61 agcgccaacg gggtatgaat tggatgcgac accagtaact ttcaagatcg aaaaagaaca
      121 agaagtggcg atctttttaa caaaagagaa tagaaaagtt gctgatagct cagatataag
      181 aggaaacaat ccaacaagca gtggaaataa gcatcttcca aaaactggag aaaccatcat
      241 tagccagttt atattgtcaa tattgggtgt gctacttgtt ttcatcagta taaaatttag
      301 aaagaaacga aatatatgag ctaaaataaa aaattgaata agcatagttt ttaaaatcaa
      361 ctagtaccca tcaattgaat ggaaactacc gattttagaa ggggtataca aacaaatatt
      421 agtatcggag tagagaaggt tgaacccaac cagtaaataa gtaaagacaa ttttgtgctg
      481 cgttgagaag tgaaaaataa acctttgtag taatactagt taataaaaaa tatacaaaaa
      541 cactgcgaaa atcattcttg agtttcgcag tgttttttgc ctaatgactc aagtttattg
      601 ggtaatactt tgattttctt tctacaaata atcagcgatc agttgtaatt ttttctctag
      661 ttttaatggc tctttgtcaa taaggagaaa aacaagtcgg gtcttttgtc tcggaaagct
      721 tcaaccaact cgtgaataaa ggttacgcct ccttgaaagg cgtggaaaaa ccaagtaatc
      781 gatcaatcac caatttcgca tttggtagaa catgtttggt gagctgaaag taggtggcgt
      841 tcatgtctgt aactaagaat tctatttctt taggattagt gcagctaagg aaatagtttg
      901 ttaatcgagg aattttttgt gtaggtaaat tttctcgatt aatttttctg tctcgccatc
      961 cgcggaaata aaactcagtt tatcctcaac ggaaacgtga gaacgaaatt cacaaccctc
     1021 aacactcaag acagaattct tttggaagac tttggtaaat agcttctcaa ctctttcaat
     1081 gtgcgaataa cggttgtcaa agatacctaa caacttttcg aaataaaaaa taacgacact
     1141 ttttcagtca gtaaagaagt gattttatgt ctgacatgat ttgaaatcga gtgccttgga
     1201 cggataaaat aactttgagc gttccaatgg cttcgacagt ttttacaggt atagcgctgc
     1261 ttttttaggc gtataaccat tggcatatga ttatattatt caaagtggac aatggtttcc
     1321 ttcttcttgt ttttcaccac cattaatttc ctatttccat ctaccacagc agaaccacaa
     1381 tttctacaga cacatggagc agggaagaga actgcagcaa cgatcaatgt ttttttcttc
     1441 tgtaaggttt cgtaagagac ctctgtaatc atcaaatttt tgtctattga tctcagaatt
     1501 tttttgatag aaccattcat aaagcatatc gttctctcag ttgtttattt tgtggtgatt
     1561 taatcataca agaaaacgat atgttttttt ataaaaacat gaataggaaa aatagttgac
     1621 aagaaaatga caacgccata tgatgttcca tagtatttac aacctgtcat agatgactgt
     1681 aaaaaataaa agtaaaaaga aaggacgaca acaggtggat ttttatcagt ctctacaact
     1741 agatccgttt attttaaaac aaaaaatacg agaagcagct tctaaaaaag aaaagtgcat
     1801 gtatatttgt tcattgtttt tacgatcatt attcatcgta ttgtttgcca tttgttttat
     1861 cattataatt acaacattat ttgaatcgaa gcataaacca tacgctgtgg ttttattctg
     1921 tatgttgatg agtattcgtt ttgttgactt tggttataag atttcccatt cgatcatagg
     1981 tttagcaatc gtcatgttct cactattagt agcaccgtat gtccagctga tcaaatggtc
     2041 agttatgggc atgttgattc attttatttt actaagcagc atattaatgg caaccgctag
     2101 tgatcccaaa atggggaatg cttctttgta cggcttttca tatctcttta ttgtttattc
     2161 gttacctaaa gattcgttga acaagaattt ctttacacaa acaagtagct tattattttt
     2221 gtttttttgt tggttttcag ttctccttta cagaaaacat cgagaaaaga acaaagacaa
     2281 aagcttgttt aaagagattt ttttgaaagg catgtattcg caggaaaaaa tttggatgct
     2341 catctatgca tttggcatta gtcttttgat cgttgcagga gagtatgtcc cttttcagcg
     2401 attgatgtgg gcagggtttg cattttcttc tattgtatct agctatggat tgatgtcgat
     2461 tggtttcaaa gaacgagcag tagaccgaat cattggctca ttgattggat gtgttttatt
     2521 tattggtatc agccaattta ttccttttaa ctgggtcggt atattaggtg gtctagcttt
     2581 aggtgtttgt tctacctata gatataaaac agtttttaat tcttttggtg ctttggcaat
     2641 cactgccagt ttattcgggg ttccaggagc agtaacgatc cgcatttttg agaatatcct
     2701 tggggtatgt ttaggaatca tgtatattgg ggtgactgaa atacttatcc gaaaaataag
     2761 agaaaaacac ggtttgaatc attagatgag atcgaataaa aaataaggaa aaagtgaaga
     2821 tacacatgga tgataaattg ctatcattcc atgtgtatct ttttacggta aatcagtgaa
     2881 acgttcgact ttcattttta atttatactt tttacggagt tcgtttattt gagccatcat
     2941 ttcggatttg tgatgctttt cgattgcttc ttcatctttc caacggtcga tcagtaaaag
     3001 agattctgga tcttctagtg gaaagtaata gtcatatttc tcgtttcctt cttctgcacg
     3061 aatcgctttg acaatgtttc tttcgaccat ttcttctgca aattttctgg cttgtccatt
     3121 tgtacttgta tacaaaatat tcactgtgat actcactaaa tcccctcctg aaaaaattct
     3181 atcactagta tatcacggtg ccagaaatta ttattcaaga tatgatacac attttttccg
     3241 caatgctacg gaaatatata ttttgaatag aaaacgctta caagatatgc ttggtttata
     3301 aaaataaaag gagtgattga tttatgaaaa aagcattgat tatttgtgcg gcaggaatgt
     3361 catcctcgat gatggcctca aagacaacgg atttctttaa gcaaaaaaga gaagaggtac
     3421 tcgtagatgc agtttcagct acagaaggcg ataatatgat taagaatagt gattttgatt
     3481 tatttctgat cagcccgcaa acgacgatgt ttttggataa attcaaaaaa ttaggagaat
     3541 ctgttggaaa gcctgttgtt agtatccctt tccaagcgta tgtcccaatc cctacaggca
     3601 tccagaagtt agcagaggtg atcgaagaga acatatgatg gaataggagt ggatgataat
     3661 gaaacagaag gaaaaattgc tgttgagata tttgattgat cacaagaagg agtatgtcac
     3721 cagtcaacgt ttagcttctg aattgttatt atctgatcgg acaatccgta attacttgca
     3781 gcggatcaaa gagttagtag aaaaaaacgg aggtaagatc attgctaaac caggttatgg
     3841 ctaccaactg catattttac aaagactgac atttgacttg tttttgagtc ggcaagaaat
     3901 agtacctgga tatgctagag aaccgcaagg attttatgag tccgaagatc gacaaaagta
     3961 tatattgaac caattgcttt tggaagatgc cattcttttt atggatgatt tggcagaaga
     4021 gctttatatc agtcgttcca gtttgacaaa ggacatgcaa gaaatcaagg agcgattgat
     4081 tccatattca ctaaaaatcg tttctaagca tggtcaaggg acatggatcg aaggggaaga
     4141 aagaaatcga cggcatttta tcatggatac tttttttgga aaccattatg gaaattcatt
     4201 gaaagaatat ttagggcaaa gccgtttttt cccagacatc agttttgaag agctagtcat
     4261 tatcattctc gatgaaacaa gagaagctaa gttaaaagtt tcggatttta tcatacagaa
     4321 tctgacttta cacttggctt tggggatcaa acggctccga gaagggtttg aaatcaagga
     4381 attaggcatc gaacaagaag tttctaagcg agtagagtat caagtggcgc agcggatcgt
     4441 tcaacgtatt gaggtaatcg caggtgtatc gtttcccgaa gaagaaattg cttatttgac
     4501 attacatctc atggctaagt cgaatcacaa agaaaatcaa aatgatcagg aactatcaaa
     4561 ggaattagcg aaagttctga aaagattgtc tcaggaattt catttttcat tagtagagga
     4621 tctccagctt ttcaatggac tactcgatca tttgtcaccg atgatgattc gattgagccg
     4681 aggtattcgc attgagaacc cattgactga ggagataaag aaagaaaatc ccaaagtctt
     4741 tgacgcagta aaaaggcatt tcagcaacat gccggctttg aaaaactata ccatcaatga
     4801 agatgaatgg gcctatttgg ctttgcatct catggcggca ttggaaaaag aaagagctgc
     4861 gcataaacta catgcattga ttatctgtgc aacgggttac ggcagtgccc aactattaaa
     4921 gaatcgcgta gtcagtgaat tcggtaaaaa tattacagtg gtaagtgtaa aaggctatta
     4981 tgagatcaat gaaacaactt taaaaggaat cgacctgatt atttcttcta ttgatctttc
     5041 gacaatgttt ttcaaaattc cagttttaca tgtcagtatt tttttgaatg aagatgatgt
     5101 tcgtaaaata cgaaaggtag ttggagaatc tataccctat cattcctcag atagaccgat
     5161 accagcattt tctctacagc aaaaaaagca gatttacacc gaacagttgt ctgaaaaatt
     5221 tttcaagact tatacgtacg aaccgaaaaa ggaagaaatt cttcatgatt tgctgcaaaa
     5281 gctgagcata aatgaagaag aaggatatat aagagaaatg cgaaagcaga tccagcaacg
     5341 tgaaaatatg ggacaaatca tattcagtga tacagtggca gtcccacatc ccgctgttcc
     5401 agttggaatc gaaacaaagg tagccgttgc attgattcct tctgggatgc agtgggatca
     5461 atatcaagaa attcattttg tttttttagt ttcgccttct tatgtcgaaa atgaaggaat
     5521 tacggtcgtg actaaagcaa tcgttaggtt agttgaccag ctaaaagtac agcaggaaat
     5581 cttagctgaa cctatttttg agaatttcag cagtaaattt atcaaactga tcgaataagg
     5641 aggaaaacag atggctgaaa aaataaacag tgaaacacta caagtgactt cttttgatat
     5701 cattttccat agcggaaatg cacggacact gatacatgaa gcatttcgct cgatgcgtaa
     5761 agagatgttt gaagaagtag aagagaagtt gcgacaagca aatgaagaga ttcttcatgc
     5821 gcatcagtca caaactcaac ttctgaaaga atatgccagc gggacaaaaa tagaaatgga
     5881 aattatcatg gtccatgcac aagatcatct gatgacgacg atgacattat tggaagttgc
     5941 acaggaaatg aattatttgt atcaaaaaaa taaatagaaa ggaaggatca ttgtgcagga
     6001 tacagtaaaa aggaaaatcg aattagaaaa aaagcaactt agtttgaaaa acatgtattt
     6061 caatcgttat ctaatcgtac gttacctgac tgcctttttc tttttcatga acatgcaatg
     6121 gatgattctg cttctatcag ctaaaagttt aggaagtagt ttaccgatga ttctgatttt
     6181 agctattttg cctgcagtag gagaacaagt aaagctttat cgaaaacacc aaacgaacgt
     6241 tccatggaca aagtggtatt ttttgttcca gagtgtcttt aatttattag tgattccagt
     6301 gttgtttacc tcaggattta cattcttgta tccttttatg gcaaataaca atggaggaca
     6361 gttatttgtt ttcattcttg ttgtaagcgg tatttttgcc agtgcattta tacagtatcg
     6421 tttaaagaaa atttcactca accaagacca acagtatatc cgtattaaac aatatgaaaa
     6481 agcgttatat ttaggaaagg agaataacta atgtttgatt ttttgcagaa atacctgatg
     6541 ggacctatgg ggaaaattgc gcaacttaaa atcgttcggg ctgtcatggc agcggggatg
     6601 gcttctattc ctttcacgat tgtaggctcg atgtttttgg ttttaaatat tttgccatta
     6661 ccgttcccat ttttagaagg attttttaat gcaacttttt tcaaagttag tgacttgtac
     6721 atgatcgtca atacaatgac gatggggatt ttatctattt actttgccat tgtgtttgct
     6781 tatgaattaa cgtcaattga acgagatgaa caaaaattaa atgttaatcc gctcacgggg
     6841 gcattactat ctgtttttgc attctttatg tgtatccctg aattgatcat gactgatggt
     6901 aaaatcagct tgattgcttc tatcaatgag acagaaactg tagtaaatgg tattaggatg
     6961 ggctcttttg tcgaacgttt aggaacttca ggaatcttta cagctattat tatgtcgatt
     7021 attgcggtcg aattgtattg tatgtgtgtc aagagaaatt gggtcataaa aatgccagat
     7081 gttgttccgc caggagtttc tcgttctttc actgctctga tcccaacatt tgtcattgcg
     7141 tttgtggtga tgatcatcaa tggattatta gttgcactag gaacggatat cttcaaaatg
     7201 attgctatcc catttgggtt tgttacgcag ttgacgaata catggcttgg tgtcatggtg
     7261 atttatttct tgatccatgc attgtggatc gtagggatcc atggagcgaa tatcatcact
     7321 tctttcttga ccccgatcgt attagctaat atggcagcta acgcacaagg cgcaaattat
     7381 ccgttggcag gagaattcaa caattcttat gtaaccgttg gtggatcagg tgcgacttta
     7441 ggattgatca tctttattgc attcctcgcg aaatctgatc agctgaaagt gttaggaaaa
     7501 gcctcacttg taccaggatt attcaatatc aatgaaccaa tcattttcgg gatgccaatc
     7561 gtctacaatc cctatttagc ggttccgttt ttcttagctc cgatggcttc tgctacactc
     7621 gcttattttg ccattcggct ggaaatcgtc aaaccgatgc ttgcacagat gccgtggcct
     7681 tcaccagtcg ggatcggagc atttgttggt agtggtggtg attggaaagc tgctgtgtta
     7741 gcagtcattt gtgcaatcat cgcattcatt atctggttcc catttatcaa attctatgat
     7801 aataaattat taaatgaaga aagagaaaag gcagcagaat tagctagtga aaagttaaaa
     7861 tgatttctaa gaaaaaagga actgtgacaa aaaatcacag tccctttttt tcttagaaat
     7921 gggaaaccac tagtatataa ataccattat tcttcaatca accgcttcac gattgcataa
     7981 tccccttcat aaggttcatc tacaccattg attttttgat agggatccac gccttttccc
     8041 gcaataacga ctgaatcttg cggagcgctt tctgctaaag cgcgtcgaat cgcttcagga
     8101 cgatccattt cataatgaac gatcaaatct ggattagtaa ttgcttcatt gatttcttcc
     8161 gcgatttttt tcggatcttc aaatgctggg tcatctgccg tcaaaaaggc gatgtcagct
     8221 gtttctgata gcacttttcc aaaatcatgt cgacgggaga ttgctttgtt tcctgggctg
     8281 cctaatacaa caattacacg gccatctgga tgttcatttt tggcaaattt cagcaatgtt
     8341 ttcaagctta gataattatg tgcatagtcc acatagacat gcgctccatt ggattggatc
     8401 aattgatcca ttcggcctgg tacacgagct tcttttaatc cgtcttgtgc atcttgactg
     8461 gaggcacctg ttagtgctgc ggcaatgatc gcactggctg cattttcttg gttgaatcct
     8521 ccagccagca aaatatcata agcagtatcg cagatagcta agcgatcttc atgagaagat
     8581 aaagtaaaac cgtgagtacc tttatctgaa cggatcactt gataattgct ttcttccgaa
     8641 cgaccataag tgatcgttgg aatctggaat agatcggctg tttccctaag taaatcaaaa
     8701 tagtcacttt catgatttaa aacaactgtt tttgaattca agatcaattg tcgcttgcaa
     8761 tagaaatagt catcaaatgt cggatgttcg atcggactga tatggtcagg cgagatattc
     8821 aagaagattc cgacatctaa agtcaagccg tatacgcgtt gtgttttgta agcttgtgaa
     8881 gaaacttcca tcactagatg agtcatacca ttttcaactg cttctgacat catacgatat
     8941 aaatctaatg actcaggcgt tgtcaaatgg gatttgaaat atgttttgcc atctaacgtt
     9001 gtattcatag tagaaagcaa tgctgttttt ttatttgttg tgtgatccaa gattgctttt
     9061 gtaaagtacg cagcagttgt tttacctttt gttccagtga atccgatcac tttcaattta
     9121 ttttgcggat agtcgtaaaa agccatagca agaagcgcca ttgctttacg gatatccgtt
     9181 acgatgattc ctactgctga attttcgtat ggttgttctg atacataata ttgaataccc
     9241 tgttcgattg ctgaatccaa atattcttct ttgaaattca ttcctttaca gaaaaacaaa
     9301 gtagaagcat cagcttttct tgaatcataa cttaactgaa gaaattcagc tttttctggt
     9361 acatcaagat gccagcctct tgttgaaaca aattctttta atagattttc ttttaatagt
     9421 aaatgacgga tgtcttctaa agagaagctc attgattgat ccctcgcttt attcaatttc
     9481 ttggtttatt atagcacact ctttttaata tatcgtttgt ttccgctatg acaaaatcca
     9541 gctttattca aattttcttg acacagggga caagcggatg agtattttca taaacaatga
     9601 actgactgta atagatagga gaacgaatag tgtctccaac aatccaaaaa cataataatt
     9661 caaaaagacg ataagactat tggtaagtaa aagtccttgc ggcattggaa gccgaaaaaa
     9721 tttgtggaaa ataatcggcg gtatcgttat accgccattt gaggccccga ctgtgtacaa
     9781 tgtgtaaacc ccaagactga acaaaagact tcctgaaatc agtgaaaaga aatgtgagga
     9841 gatcatttcc acttttggaa caatctctaa aacaacagga aaaagcagac ttcccgctac
     9901 agtcttaaaa aatactgatt ttcctaatag gacagcggct agaagcagca tcataagatt
     9961 gatgctccaa acagtaactg agcgttggat agaaaaggcc gtttcaacta aatgtccgat
    10021 ggcgcccact ccggcggaag ctatatcgtg agggagcaag aaccaattca atccaactgt
    10081 gataaagaaa agacctaaaa atataacggg tagttttttt atttcttgtg tcattgatat
    10141 ctcctataaa tgttagccat aaaaggctgt atatttcttt actttaccaa aaaaacgaaa
    10201 ccgaattcaa aaaaacaatt gttcttataa aagttatata aaatcgattg ccagtttctg
    10261 ttttaccaat taaaatgata gtaatgtttt gtagaaagtt ggaatctgac cgatggataa
    10321 tcaaagaaaa ccagcattag atcaattaac accgcaagaa aaaatggcac gaggctctgc
    10381 ttggatgacc gccagtaata tcatttcaag attgctaggc gctgtctata tcattccctg
    10441 gtatgcttgg atgggcgaaa atgctaaggc tgccaatgga ttattcaata tgggatacaa
    10501 tatatatgca ttgtttctac tgatctctac agcaggaata cctgctgcga ttgcaaaaca
    10561 aactgcacgc tataattcat tgaatgaata tggcacttca cgacgattat ttatccgtgc
    10621 attacagatg atgggcgggt taggcctgtt gtttgctttg ttcatgtaca tcgcctctcc
    10681 ttggctagct catgcttctg gtggcggaga agaactgatt ccaacgatgc gttctttgag
    10741 tatcgctgta ttggtttttc cttgtatgag tgtgatacga gggtatttgc aaggcaatca
    10801 agaaatgatg ccttatgcgc tgtcgcagat tgtagaacag gtggcacgta ttttttatat
    10861 gctattggct acattcatca tcatgaaagt catggatggt gactatgtga cggcggtcac
    10921 gcaatcaact ttcgcggcat ttatcggtat gctagcaagt attcttgtcc ttttgtattt
    10981 tttgaagaaa catcaagcgt atacatcggc gtttattcac tacagcgaaa acaaaataaa
    11041 tattgcaacg aaggaattgt tgttagatac cgttaaagaa gcgattccgt ttattattgt
    11101 gggttcaggg gtaacgatct tcaaattagt cgatcagttc acttttatga agatcatgtc
    11161 tgcatccacc gagtattcca atgcacagtt actggatctt ttttcgattt ttagtgccaa
    11221 tccagataag ttgacgatgg tagttattgc attggcaaca tcgatcgcgt ctactgggtt
    11281 accgttgatt acagaagctg ttacgttgaa agatcgagta ggtctggcaa aattgacaag
    11341 cggaaattta cagttgtttt cattttttat gtttccggcg acatttggtg ttatgttact
    11401 tgcctatccg ctaaatacat tattttatac accggattct ctaggaagta atgtactgat
    11461 ccaagcaagt tttgtaggac tcttcctagg actatatatg ctagtttcca acatgctgca
    11521 aggaatgttt gaaaacaagg cggcaattaa ttatttggtt attgggtttc tagtgaaact
    11581 ccttcttcaa tacccagcca ttcgtttatt tgaagtatac ggccctttat tggcgacaat
    11641 gatcggattt gctgtgtctt gtaccttgat cttaaagaga atccatgaag tagcccgctt
    11701 taaaccaagt tttgtctggc gcaggacatt gctgatcttt attcttacct tgattatgct
    11761 tttggcttcg tggatcacaa agatgatttt tggtgctttt ttaaacgaag atagcaaatt
    11821 ccagtctcta ttgttgattc ttgtagtagc aggtataggg gctttagtgt atggttattt
    11881 agctttgaaa atccgcttgg cagaaagact actaggacca ggaatagcgc gtttacgaca
    11941 taaattaaaa ataaaataag tgaatcagaa gagctagatt gatgctcttt tgaaatgggg
    12001 gaaacgcatg cgtttagata agtttttagc agatatggga cttggcagcc gcaaagaagt
    12061 caaaggatta atcaaaaaag gaacgatcga agtcaatgga caagtggtga aaagcgataa
    12121 agcgcaagta aatgaattcg aagatgcggt tacttattta ggagaaatca ttacgtatca
    12181 aaaagatttt tactacattt tgcataaacc tgcaggagtc atttctgcta ctcaggataa
    12241 tcatgatcaa accgtaatcg atttattgga agaccaggat tatcgggaag atctttttcc
    12301 agtcggccga ttagataaag acacagaggg cctcttgatt ttaacgaatg acggggtgtt
    12361 tgcccatcag ttactatcac ctaaaaaaca tgtggagaaa gaatatctag ctgaggttag
    12421 cggtataatg acagaagaag atatccgttt atttgcagaa ggactgatta ttgatggcga
    12481 tgagcagaca ttgccggcaa aactccaaat tgatcaagta gatcaagaga cagaaacttc
    12541 aattatccgt ctgattttgc atgaaggaaa gtttcaccaa gttaagcgaa tggtcaaagc
    12601 agtaggaaaa gaagtcttat atttaaaaag aatccgtatg ggaaaatttt ggctgcctaa
    12661 agatttgaat aaagggcaat atcgacccat gaccaaagaa gagctagaac aagtgagaga
    12721 aaaataattg aatggatagc taaaagaata agcagagtag ttgtagcgcc atctgaaaag
    12781 cagacttgtc cgtctgactt tcggatggct tttttgtgaa atttcggctt atttccgtag
    12841 aagctacttc tggaacaccg tttgttcgga ataagggact gtgacaagac ttttgttaca
    12901 gtccccattg ttccttgtga ctcagagtta gatagtaagg acacgatctc cctaatactc
    12961 aggccaaaac gctgaattca caacctctga taaacggtgt tcaatcagct gaccctcggt
    13021 attagctcaa aaaacggcaa aatatgagga actattttcc cgtttttttc tctaatactc
    13081 aggccaaaac gctgatttca caacctctcc tatactttgt ttcattttcc ctatactttg
    13141 tttatttatt ctcattattg aaagcgctat aattacgttg cgaatgaatc gaaaggggtt
    13201 tttagatgaa aaaacgaaag gggttcaatc atttttttat gaatctatgg cgttataaag
    13261 cacttgtcct gatggctgta ccgggtatgc tttggatgat ttttttcttt tacatacctg
    13321 taatggcgaa tgtcgtagca ttcaaaaatt ttcatatttc agcagatggg ttcttggcta
    13381 gcttgaggga aagtccttgg gtgggttggg aaaatttcaa atttttattt tcttcggatc
    13441 aagcatttct catcactaaa aatacaattt tatataatgt agcatttatc cttttgaatc
    13501 tgctgatctc agtcgtattt gcaatcatta tgagcgagtt gagaaataaa cgactcgtta
    13561 aagtttatca aacgatgtcc ttactacctt attttctatc ttgggtcatt atcagttact
    13621 ttgtttacgc ttttttaagt cctgacaaag gaatattcaa tcagtggatc actggacatg
    13681 gaggagaagc tgtcaattgg tataatgaac cgaaatattg gccgtttatc ttggtgttca
    13741 ttggtacatg gaaagggatt ggttataaca gcattattta ctttgcatct gttatgggaa
    13801 tcgatccgac ttattatgaa gctgctatgg ttgatggagc tagcaaatgg cagcagatca
    13861 aacatgtgac gataccacaa cttattccac taatgacgat tttaacgatt ttagccgtag
    13921 gaaacatttt ccgagctgat tttggcctgt tttataacat tcctaggaat tccggtgcat
    13981 tatatgatgt gactcaagtt ttggatacgt atatctacaa tggcctgaca tccacaggag
    14041 attttggtat gacggctgct gcaggacttt accaatcagt tgtcggcttt gtcttgttga
    14101 tgatcacaaa tacgatcgcc cgacgttttg atagcgattc tgcattattc taaggaggaa
    14161 aaaaatgaaa aaaaagaaag tggagagagt ccaagtccgc tctttcaata agacggctaa
    14221 ttttttctct aatataatga ttgcactctt tgctatttcc tgtattttgc catttatatt
    14281 tgttatcgcc atctctttta ccgatgagtc catattgtcg actcacgggt acagtttttg
    14341 gccagaaaag ttcagtatat ttggttatac gtacttgttc gaccagatgc aggataaaat
    14401 tttccaagcg cttttcgtca ctgtacttgt tactgtttta ggtacactga tcaatagcac
    14461 ggctacttct ttatatgctt atgcgatttc acggtcaaac tttccattcc gtcgtttttt
    14521 taccgttttt tgtttgatta cgatgttgtt ctcaccaggg atggtggcca attatcttgt
    14581 tatgacaaat cttcttcagt taaaagatac gatttgggca ttgatcttgc caatggctgt
    14641 cagtcccttc aatattattg tgatgcgcac attctttaaa cgttcggtct ctgattcgat
    14701 tattgaatcg gcacggatcg atggagctag cgaactgcga atatttgttc agatcgtttt
    14761 accgttagct gttccaggta ttgcgacaat cagtttattt gccgcattgg gttactggaa
    14821 tgactggttc aatgctttac tatacatcca agatgataag ctagttccac tacagtatct
    14881 tttgatgaaa atccaaagta atattcaata tctgacacaa aattcaggag caggaagcca
    14941 attgacaggc ggattagcct cagttccagg agaatctgcc agaatggcga tcgtagtcat
    15001 ctcgacgttg cctattgcga tcagctatcc atttttccag aagcattttg tcaaaggtct
    15061 aacaatcggc ggagtaaaag aataaagaaa caaaaatata aatcataaaa ttaggaggaa
    15121 gaaaaatgaa gttatggaag aaactagcct ttacaggtgt ttcagcgttg ttgttgggaa
    15181 cattagcagc atgcggaggt tctggttcta aagaccaggc agaagctagc aattcggaca
    15241 ctttgcagat gtatcaaatc ggagataaac cagataattt cgatcaattg atggaagtag
    15301 caaacaaacg aatcgaaaaa gagatcggtg taaaagtcaa tatcaattac attggctggg
    15361 gcgattacga aaagaaaatg aacgtcatca tttcttccgg ggaaaattat gatatcgcct
    15421 ttgcgaacaa ctatgtttcc aatgcccaaa aaggagcctt tgctgatttg actgaattag
    15481 cgcctaagta cgctgaaaaa gcttataatg atttagatga agcatatatt aaaggaaatc
    15541 tggtcaatgg aaaattatat gctttcccag tcaatggaaa tgtctttgct caacaagtac
    15601 ttacttttaa caaagcttta ctagataaat acgatctttc aattgatgga atccaatctt
    15661 acgctgatgc agaaaaagtc ttgaaggaat tccatgaaaa agaaccaaac acggcagctt
    15721 ttgccattgg acaaggcttc aaagtccaag ggaactttga ttatccttta gggaatacgt
    15781 taccatttgc tattgatctg gatggagatg attcgaaaat catcaaccaa tacgataatg
    15841 aaacgtatat cggactgtta cgtacaatgc atagctggta caaacaagga ctcattccaa
    15901 gtgacgctgc aacgagcaat aaggattatc cactagaagg aaatacttgg ctaatgcgtg
    15961 gagaaacaca aggaccgtat gattatggcg atacaatttt gacaaatgca gcaaaacaag
    16021 aacttgtctc aaaagcaatt actgttccac taaaatctac agctcaagca caaatggcaa
    16081 attttgtcgt atcaaatact tcaaaaaata aagaaaaatc agtagaactt ctagggctgc
    16141 ttaacagtga tccagaatta ttgaatgggc ttgtttgggg gatcgaggga gaagcatggg
    16201 aaaaagtcga tggttcagat gataaaatca aattgctaga tggttatcag ccaaatacac
    16261 atatgtctgc ttggaataca ggaaacaaca agattctata tacacaagag tctattacgg
    16321 atgacatgat tgccaaacgt gatcaatcga ttgctgatgc agaaacttca ccgattttag
    16381 gattcagctt caacactgac agtgtcaaaa cagaattaag taatatttct aatgtcatga
    16441 atcaatattt agatggattg aatacaggga ctgttgatcc agatgaaaca ttaccgaaat
    16501 tgaaagacgc tttggacaag gctggctatg ataaggtatt gaaagaaatg caaaaacaat
    16561 atgatgagtt ccgacaagaa taataatgaa taaagacaaa ggggttgtga caaaagacgt
    16621 gtcacaacct cttgttccga ataaacggtg ttcaaaagcc agtttctcgg tattaagcta
    16681 aatatatgta aaaatgtgga aagccatatt cacaaaattt ttcttactac tcgaaactaa
    16741 gcggctattt cacaaactct tggtaaacgg tgttcaatca gctaaccctc ggtattcgct
    16801 cacaaaacga caaaatatga ggaactattt ttccgtttgt ttctctaata atcaggtcaa
    16861 ggtgctgatt gcccaacctt tttttgtata atgagaaagt ataaacttaa tgtttggagg
    16921 agaagatatg ttagggaagg ttttggaaga gttatttatc cggatctggg tcattataaa
    16981 actaaccttg tatttctgga tatatacatt tgccggcggg atcatctttg gattaggtgc
    17041 ggcatggaaa acagtcaatg aactatttta tttgtacgga ttcgagtata aagaaatcac
    17101 aataaaaaga ggctggaata tatataaaag aaatttttta agaggcaatc tgctatttag
    17161 tttattttta tctggtactg ctttattgag ttacaattta tatttatctg ttcaaatcaa
    17221 agggctcatt tttttagcta ttgattttat tttacttttt gccttgctct gcctatttgc
    17281 tacttatcaa tacagtatga ttttagatag tgaatatacg atctctgttc ctaatctgct
    17341 aaagctaagt tttatttcgg ttttttccag cttttcgtct tttctaaaaa taattattgg
    17401 tagcggtata attttaggcg tgacttggca attcaaagga ttgatcctgt ttggtgtcat
    17461 tggtcttttg acggtttgga atggtacgat gacaacacat tggcgtgaag aattagataa
    17521 acagttggaa agttatgaat aagtttcgga ttctattttc aaaaggaacc ttactaaatc
    17581 gtctgttgcg gaaatacgcg gttatcatga tcggtgtaac gatgactgct accgttattt
    17641 ttagtatcca tacttgggaa caaaatcaaa aacaggcaga aaacatgaca agtgatgccg
    17701 ttcaaagcac aagcagaatg ctgaatgata aaacaacctt gagtaggatc atcaaaaacc
    17761 agcttgtagg gaattccgaa aagatcgaaa atgtgacgac ctacttaaca aaacccatag
    17821 atcagtatct gatgtatgta tacgaacagc aaaacagtac cgatgaatta gtttctttcc
    17881 caaatcaaat caaagatctc tacgcaaatt atgaagaact aagtgcgata tatatcgtgt
    17941 tgaatcaact accggagtat tatgaatctt ctagagaaaa caaaggtggg gaaataaaaa
    18001 aggggacacc aaaattacta gacgcctttt atatcaagtt gcctatcaca caaggaggag
    18061 ctgagtcagg aagtattttt attggttttc aaaagaaaga actagatatg attttagaaa
    18121 atctaacgtc attcaatgga ctctctttat acatgatttc tggaacgaca aatcgactat
    18181 acacattcca tgataataaa atagctaaag aaacgtttga aagacaagaa atgatcatca
    18241 cagagagtct gaagaaaaat tcacaattgc cgattgacga tctggaaaaa aacaattttg
    18301 ttcaacacca agaactgtct gatgattatc gtatattagc tgtactagat aaaaaaagtg
    18361 taaaaatcga aacgggaaaa aatcttgccc cgctgatcat tggtatcatt attttagaca
    18421 atatactgct tatctttctt tataaaacat tcaaacgata ttcacagcaa gtatctatcg
    18481 tcatgcaggc catggatcaa acagcagcag ggaatttaga gacgcggatc gacataacaa
    18541 acacggaata tgaactgagg gaactttcaa tcggtatcaa cgaaatgttg gatagcatca
    18601 atcagtttgt agaagacatc tacaaattag aaatcaaaca gcaagacgca catatgcggg
    18661 cgcttcaagc acagatcaat ccacactttt tatataatac attagaatat atccgaatgt
    18721 atgcaatcag cgaaggaagt gaagaattag cagatgttgt gtatgcgttt tccgctttgc
    18781 ttcgaaacaa tacgaatcaa gagaaaacga tcactttaaa agaagaactg gacttttgcg
    18841 aaaaatatgt ctatttgtat caaatgcgtt atcctaatcg agtagcctat cattttatga
    18901 tcgatcctga tttggaaaag atcgaagtac ctaaatttgt gatccagcca ttagttgaaa
    18961 attattttaa acacggtatt gattttactc gattcgataa tgcgctaagt gtcaaagtct
    19021 tgcaggaagg caaacgggta aggatcatca tcaaagataa tggaaaaggg atgactgaaa
    19081 aacggctaaa acagattgaa gaaaaactgt ctcatcctaa agtggaactt cacggttcta
    19141 tcggtctgca aaacgtgaat gaacgtttac gtgccagctt cggcagcagt tattatatgt
    19201 cgcttgagaa taatgagaca ggtggactga ccgtttctat tacattcaag gagggatgag
    19261 cgtgtatcaa gtgttattgg tagatgatga atacatgatt ttgaatgggt taaaaaagat
    19321 aattgattgg caaagtttag gctttcaaat agtagcaaca gcagagaatg ccaaagaagg
    19381 actagctgta cttgaacagc gacagattga tttagtagta acggatgtga caatgccaga
    19441 aataaatgga ctggaattta ttgaagcggc tcaaaaagaa cgtcataatt ttgaatttat
    19501 gattttatca ggataccaag aattcgatta tttgaaagga ggtatgcaat taggcgccgt
    19561 gaattatttg atgaaacccg tgaataagtt cgaattggtt gaaagcctca agaaaatcaa
    19621 aacacggtta gatcagcaaa atgaacaaaa aaatcaacaa gaaatctatc aagaactgtt
    19681 attcagtcaa tggttgaatg aagaattgga tgaaataagt gaagaagaaa taataggaca
    19741 aataagtgag aagcagcata ggatagtatt gattcaactg gcaagaactc atgatactct
    19801 tatcaataga tggttgcaag aacggcaaca aatcttttac tatcaacgaa attacggaga
    19861 ccgtgtattg atcacgttgt tgttggaggc agatgaagtt gatggcttct gttgttttgt
    19921 tcaagaaaac tttcctgatc aggaatggct gatcagtatc ggggaagaga cagattcttt
    19981 ggacaaaatc cctgaaacat tccaacaagc gaaagacaat ctacagttgc atcaatttta
    20041 tggagataaa gacgagcatg ttttttacgc agtacaagca gaaacaaaag atcaaaccat
    20101 cgatttttca agtttcagac gcgtgctgca aaataaacaa ttggaagtcg ctcaaaaaat
    20161 gatcactgat ttttttgaac agtttcagtt agcagccatg atgccggaag atatacgata
    20221 ttccagtttt ttactgttta tggagattca acgggaattg attgatctgg aagatgagga
    20281 atatttacaa ggaatcgaaa aaatccatca ggcaaaaacg gtgcaagaac ttcagcagct
    20341 acttctgtca ttcatcaaaa agcatcagag acagaaaaag tacagtgaca atgtggaaaa
    20401 agtaatcgta atcctgcacc agcattatca agaaccactt acactcaaag aagtgtcaga
    20461 aaacctgcat ttaaatgtca tgtatctagg acagctgttc aaaaaagaaa cgaaaaaaag
    20521 tttttcagct tatttgaacc atttacgtat ggaaaaagcg aaacaactgc ttttgcacag
    20581 taatcaaaac atcaatgaga tagcaagtga gattggctac aacaacacca cgtatttctc
    20641 taaattattc aagaaaatcg tcggcagatc ccctaaagag taccgagaaa acagtggaga
    20701 atcataaaag aaaaagccaa tgtaatgaaa taacttataa aaaattgtct agaaatctaa
    20761 ttttgggctg ttcttattac ttgaggcaag gcagcttttt catgacctct cgataaacgg
//
LOCUS       DACEEZ010000047        20638 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20313_2017
            SAMN12639966-rid9879223.denovo.047, whole genome shotgun sequence.
ACCESSION   DACEEZ010000047 DACEEZ010000000
VERSION     DACEEZ010000047.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639966
            Sequence Read Archive: SRR10023158
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 20638)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20638)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741683.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741683.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:44:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,016
            CDSs (total)                      :: 2,962
            Genes (coding)                    :: 2,889
            CDSs (with protein)               :: 2,889
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 36 of 73
            Pseudo Genes (incomplete)         :: 29 of 73
            Pseudo Genes (internal stop)      :: 18 of 73
            Pseudo Genes (multiple problems)  :: 8 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20638
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639966-rid9879223.denovo.047"
                     /isolate="E20313_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(90..1073)
                     /locus_tag="IWO89_001726"
     CDS             complement(90..1073)
                     /locus_tag="IWO89_001726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-quinone oxidoreductase"
                     /protein_id="HAQ0456473.1"
                     /translation="MRAISIKHPGSVDVLEQIEVPVPKPKQGELLIKVHTAAVNRTDI
                     MKRESVSLKPPYPILGVEVAGEVVENKSDISTFTPGTKVYGLVNLGGYAEYAVMPADR
                     AILLPDTLDYVSAAGISEVFLTAYQTLYWLGKLQEGETVLIHAGASGVGTAAIQLARQ
                     LSHAKIIVTAGSEEKLAFCKELGADELINYKKQDFQEEVRKITEGRGVDVILDFIGAS
                     YWEKNLASIAVDGRWVLIGMLGGTIVPEIDLGVLIGKRVQLLGTLLTPRSDQYKAELA
                     QTFMQKAGPFLENGKIRPIIDRTFQFSAVKQAHEYMEANKNIGKIILKITE"
     gene            complement(1078..1725)
                     /locus_tag="IWO89_001727"
     CDS             complement(1078..1725)
                     /locus_tag="IWO89_001727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HAQ0456474.1"
                     /translation="MKIFVVGANGQIGRHLIKDLAPSSHEIFAGVRDVASQTLVKKEN
                     VSYVSFDLTWSVEKMTEAFKGIDVLIFAAGSQGKNLLQVDLDGAIKTVIAAENAHVSR
                     YLMVSAVYADEPAKWPESMTDYYITKHYADEWLKRTNLDFVILQPVTLTNDEEVTSIQ
                     LTKPNEKASKTITRSTVAAVLAALVEETDISRTTLVLSEGSKELNTAFQEWAKEE"
     gene            complement(1810..2442)
                     /locus_tag="IWO89_001728"
     CDS             complement(1810..2442)
                     /locus_tag="IWO89_001728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HAQ0456475.1"
                     /translation="MKNTKNFTLTAMFLAILILLAVTPLGFIPIGPINATTMHIPVII
                     ASIVLGPRLGAFLGGTFGLISMIRSTVIQTPLSFVFSPFIPVIGTEHGSWKALLIAFI
                     PRILIGVVPYFVYKGLRKLMKQKADSVSLFLAGLSGSLTNTILVMNMIYFLFQQDYAK
                     VIGTNLNAVYSAILAVIFTSGIPEGIVAGLATAAVCNVLLRYFKHPSLTN"
     gene            complement(2458..3009)
                     /gene="coaC"
                     /locus_tag="IWO89_001729"
     CDS             complement(2458..3009)
                     /gene="coaC"
                     /locus_tag="IWO89_001729"
                     /EC_number="4.1.1.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopantothenoylcysteine decarboxylase"
                     /protein_id="HAQ0456476.1"
                     /translation="MKKILLGVTGSISAYKSADLTSQFVKLGAQVDVIMSKSSTHFIT
                     PLTLQSLSKRAVHTDVMQESNPQVINHIELAKQADLFLIAPATANIIGKLANGIADDL
                     LSTVAMALPVGTPKLIAPAMNTNMYQNPITQRNLETLRSVGYQEIEPRESLLACGDFG
                     KGALADIQTIIEQVMLTLNERNA"
     gene            complement(3006..3743)
                     /locus_tag="IWO89_001730"
     CDS             complement(3006..3743)
                     /locus_tag="IWO89_001730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014386989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopantothenate--cysteine ligase"
                     /protein_id="HAQ0456477.1"
                     /translation="MKILITAGGTTEKIDQVRAITNHSTGRLGQALADYLAANPDTTI
                     DYVTTKQALKPKRHSNITIYTIESALDLFLQLEELTKKEHYDAIIHSMAVSDFTPAFS
                     FSEEQLAKNLPTSSTQEELDNWFAENEQTDTTVSKISSNTEHLVLVLKKTPKIISYLR
                     EWQPKAKIIGFKLLVDVPKESLLAVAKNSLINNKTDFIFANDLTEIHGETHHGYLLSK
                     DGTVEEAQSKSEIAALITEKIRLEESK"
     gene            complement(3740..4729)
                     /locus_tag="IWO89_001731"
     CDS             complement(3740..4729)
                     /locus_tag="IWO89_001731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HAQ0456478.1"
                     /translation="MNAFRSFQVIKEPVFHTAISDLPFTELAENEVLVKIAYSDVNYK
                     DALAMSESGQVIRTYPMTPGIDLSGTVVQSNNPRFSKEDLVLATGFGLGVTHPGGYSQ
                     YQKVPGDWLVPLPKNMTLRQAMILGTAGFTAMLCVNALIRQGMTSDKKVVVTGASGGV
                     GSTAIALLHKLGFQSIIAFSRKEESVTWLKSLGALQVVRPDEFLPETTKALGKQQIDY
                     VIDTVGGEQLSSLLPLISYNGAVALCGNAGGIKLNATVLPFILRNIQLIGIDSVNVPI
                     DQRLSLWQQMADLQIADELVVQEITLDQLPETASKLLAGTHQGRTLVNVGDHK"
     gene            4987..5529
                     /locus_tag="IWO89_001732"
     CDS             4987..5529
                     /locus_tag="IWO89_001732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAQ0456479.1"
                     /translation="MEIGEKLRNLRIQKNLTQEELGERTDLSKGYISQLERDLSSPSM
                     ETFFSILEVLGVTPEEFFHQEAANLQVVYSKEDHTVYFDEENGYELEWLVADSNEKEM
                     EPVLLTFEGAGEYKTFEPSLSETFIFVLEGELELSLGETVYVAKAGQSIYYQATKQHQ
                     LRNHLKKRTKVLIVATQSYL"
     gene            5546..6631
                     /locus_tag="IWO89_001733"
     CDS             5546..6631
                     /locus_tag="IWO89_001733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016182253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ0456480.1"
                     /translation="MGKPIISFDQVVKRYDDETVLKKVSFEIEQGKFYTLLGPSGCGK
                     TTILRIIAGFTEATEGDIYFEGKRLNDLPANKRQVNTVFQDYALFPHMNVFDNVAFGL
                     KIKKMSKSDIEKKVKDALRMVQLPGYEKREISEMSGGQRQRVAIARAIVNEPKVLLLD
                     EPLSALDLKLRTDMQYELRDLQQRLGITFIFVTHDQEEALAMSDEIFVMNKGKIVQSG
                     TPVDIYDEPINHFVADFVGESNIVDGVMIEDNLVEFVGKQFECVDGGMRLNEPVEVVL
                     RPEDLTITTPDKGKLVVTVDTQLFRGVHYEIICYDEQQNEWMVHSTKKAKEGSKVGLA
                     FEPEDIHVMRFNESEEEFDARLDSYEE"
     gene            6643..7449
                     /locus_tag="IWO89_001734"
     CDS             6643..7449
                     /locus_tag="IWO89_001734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAQ0456481.1"
                     /translation="MKTMRRIYSVPYILWLGLFVIAPVLMIIYQSFFDMNGQFTLNNY
                     LSYFTSGTSLSMTINSVWYAFLITFFTLIISYPTAYFLTKLKHKQLWLMLIILPTWVN
                     LLLKAYAFIGIFSVHGSVNHFFDFIGIGPKQILFTDFSFLFVATYIEIPFMIMPIFNA
                     LEEMNPSLISASRDLGANSFETFRRVIFPLSLNGVKSGVQAVFIPSLSLFMLTRLIGG
                     NRVITLGTAIEQHFLVTQNWGMGSTIGVILIIAMFIVMLLTGEKKKGGRS"
     gene            7450..8277
                     /locus_tag="IWO89_001735"
     CDS             7450..8277
                     /locus_tag="IWO89_001735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAQ0456482.1"
                     /translation="MKKFKWSYLYLGIVFLFLYAPIFYLIFYSFNEGGTMNSFTGFTW
                     ENYEAVFEDTRLIIIVLNTFMLAFLSALIATIIGTFGAMGIYYTKKRRARTALLSMNN
                     ILLVSPDVIIGASFLIFFTLLGFGLGFQSVLLSHIAFSIPIVVLMVLPKLQEMNDSMV
                     DAARDLGANNFQVVKNIILPYLSPGIIAGYFMAFTYSLDDFAVTFFVTGNGFTTLSVE
                     IYSRARQGISLEINALSALVFLFSMILVIGYYFISKENRPKRLKKNRRVREGVARLR"
     gene            8274..9347
                     /locus_tag="IWO89_001736"
     CDS             8274..9347
                     /locus_tag="IWO89_001736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HAQ0456483.1"
                     /translation="MKKLQSLFLGILIIIAVLLLGVRQLEKTSGMSGAKVLTIYNWGD
                     YIDPSLITKFEKEYGYKVNYETFDSNEAMFTKIQQGGTNYDIAIPSEYMIQKMIKEKL
                     VLPLDHSKIKGLNNIDPRFLDLDFDPDNQYSIPYFWGTLGIVYNDKFFDANQIKHWDD
                     LWKPELKDSLMLIDGAREVMGLSLNSLGYSLNSKNMAQLTEAANKLNHLTPNVKAIVA
                     DEIKMYMINEEASVAVTFSGEAADMMWENEHLHYVIPPEGSNLWFDNIVMPKTVKNKE
                     GAYDFINFMLEPENAAQNAEYIGYSTPNEEAKALLPKDISSDEQFYPSDDTISHLEVY
                     KDLGSKYLGIYNDLFLEFKMYRR"
     gene            9775..12471
                     /locus_tag="IWO89_001737"
     CDS             9775..12471
                     /locus_tag="IWO89_001737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAQ0456484.1"
                     /translation="MEYKYRIDILQPIVYYAFRHIKLEEKMKKSAKDRVFDELIRLTG
                     NMSSVTAEKISKNLGISRQNASHYLTRLVEDKKVEKLPGKPVLWKPLDEYAVVDNTEQ
                     INEAFHSVVGHDGSLREVIQKCIAAVKYPPNGLSVLINGATGVGKSFLATKIFEYAIH
                     EQIIEKDAPFAILNCADYADNPELLSATLFGYKKGAFTGAEKDTDGLLATANNGYLFL
                     DEVHRLSKENQEKLFLFIDTGNYRPVGENVNWHSAKVRFIFATTEKGENYLLDTFDRR
                     IQISVMLPTFEDRPIRERLELIQLFFQNEADVLQKDIIVSREALSLMLSHPFSGNIGK
                     LKNIIKISCADVYSRTQEEPLTIGKNEILIQLNMLESEVESLPIASLLIKKNQKIKTD
                     RIKPFDYDSILEKITQLINEGNFNENFSSIKNEVQHLVHHIDKDSFLSMSRKLMIQMF
                     RKVWVSVLGKTYGLTTSGPVAEIISRSYVISPSYKTDLTGINRTLAANLPRTTYLCNQ
                     FLEHLPPLSEKDENFLRLLIVVTLSDYVDESIELKGLLLAHGDSTASSIQAVVNQLCG
                     NYVFEALDMPVESNVSEIVESTRNFVKRQVNTDSLVLIVDMGSLNQIYTQIKNDLKGE
                     LLLLNNLTTSIALDIGLKMTMNAPFKQIAEDAADKYSINVQYFEGFSQSSNIIISCMS
                     GLGISDKLKDVFRHHLSDESIEVFTKDYRELRELIDQNDETYFDKTKLVITTSDLPRS
                     FSIPNINVYDILDGDGAANLEHILSPEMKPAEFNRLIQELVKFFSLEGIADRLNFLNP
                     TIVMSEVETVISKYENYYHFTLNGRVKLNLYMHTALMMERLFLARTEKSEEPNYDLKP
                     DEDEFYYVSRSIFQSMEMKYNFKVNAYELSLLYELLGPYIQK"
     gene            12548..13036
                     /locus_tag="IWO89_001738"
     CDS             12548..13036
                     /locus_tag="IWO89_001738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HAQ0456485.1"
                     /translation="MFLKVGTGIAYIKVNRKKGINHMKKILIATHGHLASGFLSSIQL
                     LTGKVQEITVINAYVDECDFEQELNVFVNQVNEQDQIFVFTDLFGGSVNQKITKTFLE
                     KEIAVTILSGFNLPILLEIVLATKELTKEDIQELVNKCQQELKMTILDEVHVVNEEED
                     FF"
     gene            13048..13542
                     /locus_tag="IWO89_001739"
     CDS             13048..13542
                     /locus_tag="IWO89_001739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAQ0456486.1"
                     /translation="MIKAVRIDERLIHGQVAMTWTKSLNLTGLVVASDEAATNDIQKM
                     TLKMAAPSNVKVIIKTVDDAIHLLKDPRAKDMRLMVLVPNVKDAVKVAKAFPTEIELM
                     NVGNAGKMTPPSEDKKTLSKEVMLTTSEIQDLKELVELYPDTVFQPTPAMEKRSAISI
                     LSDF"
     gene            13563..14372
                     /locus_tag="IWO89_001740"
     CDS             13563..14372
                     /locus_tag="IWO89_001740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAQ0456487.1"
                     /translation="MYDAFVVALAVFIGIAGHEMFGMSMLSRPIVVAPLAGALMGDLQ
                     TGLIVGASLEAIFMGVVNIGISSTAEPSLAAGLATAFAIKMGGNVDAIIPIVFPLAVL
                     GLQLMNMIFSFVCGPMAAKFFKFAKSGDQKGIINLHFFMWFVHYALYALIPFFAVLFG
                     ADVVKSVLDNIPEVIMNGLTVAGNLLPAVGMAMLLRMLWSKNIAVWFFFGAVVMAYLE
                     LPLIAIAAVGTIIAIVIAQRDLQLKNISVHAVQSDAAVNATLDQEEEDFFQ"
     gene            14369..15190
                     /locus_tag="IWO89_001741"
     CDS             14369..15190
                     /locus_tag="IWO89_001741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010755513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HAQ0456488.1"
                     /translation="MKLADGISKDEKKLVRKMFWRSMTMYASVNPVTMGGGGFAYSMM
                     PFINHFYKSEEDRAAALERHTQYFSTTIPCASFVMGIAASMEKENSEKAGFDVESINA
                     IKTSLMGPLAGIGDSLFWGVWRVVTAGLAINLANAGNILAPIIFLVMFNIPNYLMRYY
                     GGVLGYSLGSKYIEKLYANGLIEILTKAASILGLIMVGAMTISMVKFNTVLSFSMKGE
                     ELMNVQEILDSIFKGIVPLLVTLSCFKLLDKKVGVIKIIFGMVVLGILLSLLGIA"
     gene            15241..17340
                     /locus_tag="IWO89_001742"
     CDS             15241..17340
                     /locus_tag="IWO89_001742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase"
                     /protein_id="HAQ0456489.1"
                     /translation="MTINEVIYSPKHPFFNAELNDGNVTTSNEYSYYRNIPDVKGLTF
                     DVSNQKTLANLDVCGTIQTLTFYRENLLTEEKPGVWVNKQLSQTNSLSLKVEVNGIVY
                     HLSESDHRIEVDVIQDCLPRYIHHYSTFKVIVVSFAPILANKRLSMLVQQVYIVNETH
                     ESLQVSVKDVPSYQEKYSDQQNVLISQKGNKDTIAQQEVASFAIAFVDPNAFEEVMTF
                     QESDIEKWLKETLNYFAQLFGQLSMPNDRVVHLYRRALYQSFSSFGMNRQGQVVGSNW
                     GSYPATNRIWNKDMYYASLPFTMFEPELCQKTVLWFDQYGVKFPGSKFTGGINHSLSN
                     SLSSALLASLYFEHTGDTKFFERCPQVLLNASRIIEDILAQRRSGEPMLFPSVWLSDA
                     FALGKYHTGSNLCLWKACIGLAEIFEALEQLSLSKRYKNIACKLKESICKQMTIDGPF
                     GEQFLEGIGDEEKTTYSVKHYQKPILEQGLIFLSDVIVDGKINLLMHDGEESDTTLMP
                     FYQFLDNKDHLYQNTMAFSASSFNPTYSEEIKGITWGLESGATFPGFITVLMSELHNP
                     QAFVTRLEELICLADLDGSWWWWPYRLEAQQGEVVRDFGCGKCGWASGLFVSLMISQY
                     FGLKFKKGVLQIDPVDNLTFSWKNLKFGQAFISIECTQSELSISNLSEKPLKISDTKK
                     ILHVEKGKTIHIQRRKR"
     gene            17337..18455
                     /locus_tag="IWO89_001743"
     CDS             17337..18455
                     /locus_tag="IWO89_001743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2961 domain-containing protein"
                     /protein_id="HAQ0456490.1"
                     /translation="MSILKNITQFKKIQSRAITPENPTGEKGKAAMLPSELGPSRKGR
                     GAIPLTSGEETVIADITGTGIIRHMWMTIRDRTEFGSFVLRDLILRIYWDGSDSPAVE
                     SPLGDFFCNGFGERCEVNSLPIVVNPTGGFNSYFEMPFQTSAKITIENLHPKDMSSFF
                     YTINYALVDELEENSLYFHANWHRERITKLQEDYKLIDRLKGQGYYVGTYLALTCLER
                     YWWGEGEFKFYIDGDSEYPTVTSTGSEDYFGGAWAFHEYDEFGRPHAKNYSTAFLGYP
                     YQSNRDATRDRFETGKLNAVHAFGDDAMPRHGLYRWHLQDPICFTEDLSVTLQQIGND
                     DVRLFERQDDVASVAYWYSTEASGNTKRHFTDRERLPR"
     gene            18494..19372
                     /locus_tag="IWO89_001744"
     CDS             18494..19372
                     /locus_tag="IWO89_001744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HAQ0456491.1"
                     /translation="MIYGAIEAGGTKFVCAVGTEELTILERVSFPTTTPVETMKQVID
                     FFKPFKEQLAGIGVGCFGPIDIQCKSTSFGHITSTPKLAWQNFNFVGTLKQYFNIPIE
                     WTTDVNAACYGEYVAGSGKGLAGVAYFTVGTGIGGGALFNGTFVEGFSHPEMGHMLIK
                     RHPKDNFSGNCPFHQDCLEGMAAGPAIEKRLGVKGQNLLADDSFWQIEAFYLAQCAYN
                     TTLMFSPDRIIFGGGVMKQEHMKKKVQDKFVELINGYVEIPPIDSYIITPELGDNAGI
                     IGGLALARKAVRNKQP"
     gene            19563..19715
                     /locus_tag="IWO89_001745"
     CDS             19563..19715
                     /locus_tag="IWO89_001745"
                     /inference="COORDINATES: protein motif:HMM:NF016526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0456492.1"
                     /translation="MRIFTGGVHLNSNILCYFMSVVIVIVAYQCMFIFRNVDFIILGL
                     MFEFFW"
BASE COUNT         6314 a         3556 c         4391 g         6377 t
ORIGIN      
        1 aaaaatttga aaagcaattt tcgtgaattt cttcttattt cttggagtta aacgcttctg
       61 tcccagcctc ttttttgatc agcatgattt tattcagtta tcttcaaaat gatttttccg
      121 atgtttttgt ttgcttccat atattcatgc gcttgtttga ctgcgctgaa ttggaatgta
      181 cggtcaatga ttggccgaat cttgccattt tctagaaaag gcccagcttt ttgcataaaa
      241 gtttgtgcga gctctgcttt atactgatca cttctaggtg tcaataatgt accaagcagt
      301 tggacacgtt tcccaatcaa tacaccaagg tctatctcag gcacgatggt cccacctagc
      361 ataccaatca agacccaacg tccatcaacg gctatagaag ctaaattctt ttcccagtaa
      421 gaagcgccga tgaaatccaa aataacatcg actccccgac cttcagtgat ttttcgtacc
      481 tcttcttgaa agtcttgttt tttatagttg atcaattcat ctgcaccaag ttctttacaa
      541 aaggcgagtt tttcttctga tcctgcagta acgatgattt tcgcgtgaga aagctgtcta
      601 gctaattgga tcgctgctgt tcccacacca cttgctcctg catggatcaa gacagtttcg
      661 ccttcttgta attttccaag ccagtaaagt gtttgataag cagtcaaaaa aacttctgag
      721 attccagcag cagaaacata gtctagcgta tcagggagaa ggattgcacg atcggcaggc
      781 atcacagcat attcggcata accacctaga ttgactaatc cgtatacttt tgtcccagga
      841 gtaaaagtgg agatatcact tttattctca actacctctc cagctacttc gactcccaaa
      901 atagggtagg gaggtttcaa agagactgat tctcttttca taatatctgt tcgattgaca
      961 gctgctgtat ggactttgat cagtaattcg ccttgttttg gtttaggaac aggcacttca
     1021 atttgttcta atacatctac agaaccagga tgcttgattg aaatggcacg catggatcta
     1081 ttcctccttt gcccattctt ggaatgctgt attcaactct ttgctacctt cagaaagtac
     1141 tagagtcgta cgagaaatat ccgtttcttc cactagagcg gcaagtacag cagcaactgt
     1201 agagcgagtg atcgtttttg atgccttctc attaggctta gttaattgaa tggaagtaac
     1261 ttcttcatcg ttagtcaatg tgactggttg aagaatgaca aaatctaagt tagttcgttt
     1321 cagccattca tctgcataat gtttagtaat ataataatct gtcatggact caggccattt
     1381 tgctggttca tctgcataaa cagcactgac catcagatag cgagagacat gcgcattttc
     1441 agctgcgatc actgttttga ttgcgccatc caagtctact tgcagaagat tctttccttg
     1501 agagccggca gcaaaaatca atacatcgat gcctttgaat gcttcggtca ttttttccac
     1561 ggaccaagtt aaatcaaaag agacatacga aacattctct ttttttacta atgtttggga
     1621 agcaacatct ctcacccctg caaaaatttc atgggaactt ggagctaaat cttttatcag
     1681 gtgtcggcca atttgtccgt ttgcacctac aacaaaaatt ttcatttgga tcatcctttc
     1741 gacgtcaaat ctttccattc aatagtaaca ccaaaagaga aaaaaatcag agatcggcta
     1801 cgatgactat cagttagtaa gagaagggtg cttgaagtat cttaacaaga cattgcatac
     1861 tgctgcagtc gcaagacctg cgacgattcc ttcaggtata ccacttgtaa aaataaccgc
     1921 tagaatagct gaataaacag catttaaatt tgttccgatg actttggcat aatcttgctg
     1981 gaataaaaag tagatcatat tcatcactaa aatcgtattg gtcaatgaac cggaaagtcc
     2041 agctaagaat aaagaaacgg aatctgcttt ttgtttcatt agtttgcgca gacctttata
     2101 aacgaaataa ggaaccaccc caatcaagat gcgaggaata aacgcgatca gcaaggcttt
     2161 ccagcttcca tgttctgttc cgatgactgg gataaacgga gaaaagacaa aagacaaagg
     2221 tgtttggata actgtgctac ggatcatgct gatcagtcca aatgtaccgc caagaaaagc
     2281 acctagtctt ggtcctaaaa cgatcgaagc aatgattaca ggaatatgca tggttgttgc
     2341 attgattgga ccgataggga taaaacctaa aggcgtaaca gctaataaaa tcaaaatagc
     2401 taaaaacatc gcagttaatg taaagttttt tgtatttttc ataagtactc cttttttcta
     2461 agcatttctt tcattaagag tcagcatgac ctgttcgatg atcgtctgaa tatcagctaa
     2521 ggcacctttt ccaaaatctc cacaagctaa aagagattct cttggttcaa tttcctgata
     2581 gccgacagat ctcaatgttt ccagattacg ctgagtgatc gggttttgat acatattcgt
     2641 gttcatggca ggggcaatca attttggtgt tcccactgga agcgccattg cgactgttga
     2701 aagaagatcg tctgcaatgc cgtttgctag cttgccgata atgtttgctg tagcaggcgc
     2761 gatgagaaac aagtcagctt gttttgctag ttcgatatga ttgattacct gcggatttga
     2821 ttcttgcatg acgtctgtat ggactgcgcg cttcgacaga gattgcaagg ttaatggcgt
     2881 gataaaatgt gtgctgcttt tagacataat cacatctacc tgggcaccta atttgacgaa
     2941 ttgacttgta agatcagcgc ttttgtaagc agaaatactt ccagtaacgc ctagtaagat
     3001 ctttttcatt ttgattcctc cagtctaatt ttctcagtaa tcaatgcagc aatctcagat
     3061 ttactttgag cttcttccac tgttccatct ttagataaga gatagccgtg gtgtgtctcg
     3121 ccatggattt ccgtcaaatc atttgcgaaa ataaaatcag ttttgttatt tatcagacta
     3181 ttttttgcga ctgccagaag agattccttt ggtacatcta ctaatagttt gaaaccaatg
     3241 atttttgctt ttggctgcca ttcacgtaga taagaaatga tttttggtgt tttcttcaaa
     3301 acgagcacaa gatgctcggt gttagaagag atctttgaaa cagtggtatc tgtctgttca
     3361 ttttccgcaa accaattgtc cagttcttct tgcgttgaag aagtgggtag gttttttgcc
     3421 agctgttctt ctgaaaaact aaaagcagga gtaaaatcac tgactgccat actgtggata
     3481 atcgcatcgt agtgttcttt tttcgttagt tcttctaatt gtaaaaataa gtccagtgca
     3541 gattcgatcg tataaatcgt tatattggag tgtcttttcg gtttcagtgc ttgtttcgta
     3601 gtcacgtaat cgattgtggt atccggattg gcagctaaat aatcagctaa tgcttgccct
     3661 aatcgtcctg tcgaatgatt tgtaatagca cgtacttgat cgattttttc agtcgttcct
     3721 ccagctgtga ttagaatctt catttatggt cacctacatt tactagtgtt cttccttggt
     3781 gtgttccagc aagtagttta gatgctgttt caggcaattg atccagagta atctcttgga
     3841 cgaccagttc atctgcaatc tgcaaatccg ccatctgttg ccataaagaa agacgttgat
     3901 ctattggtac attgacagaa tcaattccaa tcaactggat gtttcgcaga ataaaaggca
     3961 agacagtcgc gtttagtttg attcccccag cattgccaca taaagctact gcaccgttat
     4021 aagagatcaa tggaagaaga ctgcttagct gctcgccacc tactgtgtcg atgacatagt
     4081 cgatttgctg ttttcctaaa gcttttgttg tctctggcag aaattcatca ggtcgtacga
     4141 cttgcaaagc acctagactt tttagccatg tgacagactc ttcttttctg gaaaaggcaa
     4201 tgattgactg gaatcctagt ttatgtaaca gggctatcgc tgtactgcct acaccgccag
     4261 aagcaccagt aacgacaact tttttatcgg aggtcatacc ttgacggatt aaagcgttca
     4321 cacacaacat agcagtaaac cctgctgtac caaggatcat cgcttgacgc agcgtcatat
     4381 tttttggtag aggaactaac cagtctcctg gtactttttg atattggcta taaccgcctg
     4441 gatgggtgac gccaagccca aaaccagtag caagtacaag atcttctttt gaaaaacgtg
     4501 gattgttact ttggacgaca gttccggata aatcaatccc tggagtcatc ggatatgttc
     4561 gtatcacctg accagactcg ctcattgcca atgcatcttt ataattgaca tcagaatagg
     4621 caatttttac caagacttca ttttcagcca actcagtaaa tggcagatca ctgattgctg
     4681 tatggaaaac aggttccttt attacctgaa aagatcgaaa agcgttcatt cgtgagacac
     4741 tccttttaaa attttctttt atcataccat aaagcctact cttttattct tgttcttccg
     4801 taaatttaga caaaaattag gtgaaaaata aagaatcaaa caactagaat ttattccaaa
     4861 agataaagaa atattcagca aaaaaaattt tacaaagggt tgcctttttc aataaatttc
     4921 gtatactaat acgagtggta gtaaacagga agtttactat tagaaaactt cgattcggag
     4981 gaaattatgg aaattggtga gaagttaaga aatttgcgca tccaaaagaa tttgacacaa
     5041 gaagaactag gcgaacgcac agatttgtca aaagggtata tttcccagtt ggaacgagat
     5101 ttaagttcac catcaatgga aacattcttt tctattcttg aagtcttagg tgtgactcca
     5161 gaagaatttt ttcatcaaga agcagcgaat ctgcaagtgg tttattcaaa agaagatcat
     5221 acggtttatt tcgatgaaga aaacgggtat gaattggaat ggctcgtggc tgattcgaat
     5281 gaaaaagaaa tggagcctgt cttgctgact tttgaaggtg caggagaata taagacgttt
     5341 gaaccttcat tatctgaaac gtttattttt gtattagaag gggaattaga gttaagctta
     5401 ggagagacgg tctatgtggc aaaagcagga cagtcgatct attatcaagc gacaaaacaa
     5461 catcaattaa ggaatcatct caaaaaacga acaaaagtat tgattgttgc aacgcaatca
     5521 tatttgtaat atagggggta aaaacgtggg gaaaccaatc atttcatttg atcaagtagt
     5581 gaaacgctat gatgatgaaa cagtattaaa aaaagtaagt tttgagatcg aacaaggaaa
     5641 attttataca ctgcttggtc cttccggctg tggtaaaaca acgatcctac gtattatagc
     5701 tggattcacc gaagctacag aaggggacat ctattttgaa gggaaacggt tgaatgattt
     5761 gccagctaat aagcgacaag taaatacagt cttccaagat tatgcacttt ttcctcatat
     5821 gaatgttttt gataacgttg cttttggatt gaagatcaag aaaatgtcca aatcagatat
     5881 cgaaaagaag gtaaaagatg ctttgcgtat ggtccagctt cctggatacg aaaaacggga
     5941 gattagtgaa atgtctggtg gtcaaagaca gcgtgtagcg attgcacgag caattgtaaa
     6001 tgaaccgaaa gtattgctat tagatgaacc gttatcggcg cttgatttga aactacgtac
     6061 agatatgcag tatgaactgc gggatctgca acaacgatta ggaatcacgt ttatttttgt
     6121 tactcatgac caagaagaag cgttggcaat gagtgatgag atttttgtca tgaataaagg
     6181 gaaaatcgtc caaagtggta cacccgtaga tatttatgat gagccaatca atcatttcgt
     6241 tgctgatttt gttggggaga gcaatatcgt ggacggtgta atgatcgaag ataatctagt
     6301 tgagtttgtt ggtaagcaat ttgaatgtgt cgatggcggg atgcgtctaa atgaaccagt
     6361 tgaagttgtt ttacgcccag aggatttgac gatcacgaca ccagataaag gaaaactagt
     6421 cgtaacggtc gatactcagc tgtttcgtgg tgtccattat gaaatcatct gttatgatga
     6481 acagcaaaat gaatggatgg tccattcaac gaaaaaagct aaagaaggaa gcaaagtagg
     6541 attggccttt gagccagaag atatccatgt catgcgtttc aacgagtctg aagaagaatt
     6601 cgatgcacgt cttgacagct acgaagaata ggggggacaa gaatgaaaac aatgcgcagg
     6661 atctattccg tcccttatat tttgtggctc ggtttattcg tgatcgcgcc tgtattaatg
     6721 atcatttatc aatctttttt tgatatgaat gggcaattca cgctgaataa ttatctatct
     6781 tattttacgt cggggacatc gctttctatg acgatcaatt ctgtatggta tgcatttttg
     6841 atcacgtttt tcacgctgat catcagttac ccgactgctt attttttaac aaaactgaag
     6901 cacaagcaat tatggctgat gctgattatt ttgcctacat gggtgaatct gctgcttaaa
     6961 gcttacgcat ttattgggat tttcagtgtg cacggcagcg tgaatcattt ctttgatttt
     7021 atcgggatcg ggccgaaaca gatattattc acagacttca gttttctgtt cgtggcaacg
     7081 tatatcgaga ttccatttat gattatgcca attttcaatg cattggaaga gatgaatcct
     7141 tctttgatca gtgctagtag agatttaggc gcgaatagct ttgagacctt ccgacgagtg
     7201 atttttccgt tatcattaaa tggtgtgaaa agtggtgtcc aagcagtatt tattccttct
     7261 ctttctttat tcatgctgac tcgtctgatt ggcggaaatc gggtgatcac tttaggaacg
     7321 gctatcgaac agcatttctt ggttacacaa aactggggga tgggttctac aatcggtgtg
     7381 attttaatca ttgctatgtt catcgtgatg ctcctgacag gcgagaagaa aaaaggaggt
     7441 cgctcataaa tgaaaaaatt taaatggtcg tatctttatt tagggatcgt ctttttattc
     7501 ttatatgcac cgatcttcta tctgattttt tattccttca atgaaggagg aacgatgaac
     7561 agctttacag gatttacttg ggaaaattat gaagctgttt ttgaagatac acgtttgatc
     7621 attatcgtat tgaatacgtt catgctggct ttcttatctg cattgatcgc cacgatcatc
     7681 gggacatttg gggctatggg aatctattac acgaaaaaac gacgtgcacg gacagcgttg
     7741 ttgagcatga ataacatctt attagtctct cctgatgtta ttatcggtgc tagtttcttg
     7801 attttcttta cactactagg attcggatta ggttttcaga gtgtattgct ttcccatatt
     7861 gctttcagca ttccaatcgt cgtcttgatg gtcttgccaa aattgcagga aatgaacgat
     7921 tcaatggtcg atgcggctag agaccttggt gctaataatt tccaagtagt caaaaatatc
     7981 attttgcctt atctttctcc aggaatcata gctggatatt ttatggcatt tacctattct
     8041 cttgatgatt ttgcggttac atttttcgtc acaggaaatg gctttacgac gctctcagta
     8101 gagatctatt ctcgtgcaag acaaggaatc agtttagaaa tcaatgcgct aagtgcgtta
     8161 gttttcttgt tctccatgat attggtcatt ggatactact ttattagtaa agaaaaccgt
     8221 ccaaaacggc tgaagaaaaa cagaagagta cgcgaggggg tggcacggct gcgatgaaaa
     8281 aattacaatc tctgttttta ggtattctca tcattattgc ggtgctgctg cttggtgttc
     8341 gtcaacttga aaaaacaagt ggcatgagtg gagcaaaggt acttaccatc tataactggg
     8401 gagattatat cgatcctagt ttgatcacga aatttgaaaa agaatatgga tacaaagtga
     8461 attatgaaac atttgattct aatgaagcga tgttcacaaa aatccagcaa ggtgggacaa
     8521 actacgacat cgctatacca agtgaatata tgatccaaaa aatgatcaaa gaaaaactag
     8581 tacttccatt agatcattcg aagatcaaag gattaaataa tattgatcca cgatttttag
     8641 acttggattt tgatccagat aatcagtatt cgattccata tttttgggga actttaggga
     8701 tcgtatataa cgataaattc tttgatgcaa atcaaattaa gcattgggat gatttgtgga
     8761 agccggaatt aaaagacagt ttgatgctga ttgacggtgc acgagaagtt atggggcttt
     8821 ctttgaatag tttaggctat tctttgaata gtaagaatat ggcacagttg acagaagcag
     8881 caaataaatt gaatcattta acaccaaatg tcaaagcaat cgtggcagat gagatcaaaa
     8941 tgtatatgat caatgaagaa gcctccgtgg cagtgacatt ttctggcgaa gctgccgata
     9001 tgatgtggga aaatgaacat ttacattatg tgatcccacc agaaggatcc aatctttggt
     9061 ttgataatat cgttatgcca aaaaccgtga aaaataaaga aggtgcctat gatttcatca
     9121 actttatgct agaaccggaa aatgcggcac aaaatgcaga gtatattggt tattctacac
     9181 caaatgaaga agcaaaagcg ttattgccaa aagacatttc gtcggacgaa caattctatc
     9241 catctgatga cacgatctcc catcttgaag tctacaaaga tctaggatcg aaatacctag
     9301 gcatctataa tgacctattc ttagaattca agatgtatag gagataatgg caaaactcag
     9361 taagaatggg gacttatcaa cgttctaaag agctaaaaat cgtccttttt ctatctgagt
     9421 tttccaatct tctaccaaaa gcggatgaaa cgccgctttt tttgtgttat tttatttttt
     9481 ctaccacgtt tttctaccag agaaaagtgg acaaaaccat tgtaaaatag ggcccgagac
     9541 gggctagtaa atctaagatt gaaaaagtta cagaaggata aagtaattgg aaactatgca
     9601 aatatactta acccttctgt aatctattaa taaaactaat atatgaatcc agttcaatat
     9661 ttgtagctat gaaaaaacat aaaaatcata ttagataaaa actaaattgg tagataaatt
     9721 attttgtcag atatctgagg caaatattcg actatttgtg tttcaaagta aaaattggaa
     9781 tacaaatata gaatagacat attgcagccg atagtttatt atgcttttag acacattaaa
     9841 ctggaggaaa aaatgaaaaa gagcgctaaa gatcgagttt ttgatgagct gatacgacta
     9901 acagggaata tgtcttctgt cactgccgag aaaatttcga agaatctagg tatctctcgt
     9961 cagaatgcca gccattattt gacgagatta gtggaagata aaaaggtcga gaaattaccg
    10021 ggaaagcctg ttttgtggaa gcctttagat gaatatgcgg ttgtagataa tacggagcaa
    10081 ataaacgaag cctttcattc tgtagttggt catgacggaa gtctgagaga ggtcattcaa
    10141 aaatgtatag ccgctgtcaa atatccgcct aacggactca gtgtattaat aaatggagcc
    10201 acgggtgttg gaaaaagttt tttagccaca aaaatttttg aatacgctat ccatgagcaa
    10261 attattgaaa aagatgcacc atttgctatc ttaaactgtg ccgattatgc ggacaatccc
    10321 gaactgcttt cggcaacact gtttggatac aagaaggggg cctttacggg ggctgaaaag
    10381 gataccgatg gtttattggc aacggccaac aacggctatc tttttctcga tgaggtccat
    10441 cgcctatcga aagaaaatca agaaaagctc tttctattta ttgatactgg aaactatcgt
    10501 ccagtcggtg aaaatgtaaa ttggcattct gctaaggttc gttttatttt cgccacgacg
    10561 gaaaaaggtg aaaattattt attagacacc tttgatcggc gtattcaaat tagtgttatg
    10621 ttgccaactt ttgaagatcg ccccattcga gaacgattag agctgattca attatttttt
    10681 caaaatgaag cggatgtttt acaaaaagat attattgttt caagagaagc cttgtcttta
    10741 atgttatctc atccctttag tgggaatatt ggtaagctga agaatatcat taaaatcagt
    10801 tgtgcggatg tgtatagtcg aacacaagaa gaaccattaa cgattgggaa aaatgagatc
    10861 ttgattcagt taaatatgct ggaatccgaa gtagaaagct tgccgattgc tagtttattg
    10921 ataaagaaaa atcaaaagat aaaaacggat cgaatcaaac cgtttgatta tgattcgatt
    10981 ttggaaaaaa ttactcagct gataaatgaa ggtaatttca atgaaaattt ttcttcgata
    11041 aagaatgaag tacagcattt ggtgcatcac attgataagg attctttttt atcaatgagt
    11101 agaaaattga tgattcagat gtttagaaag gtctgggtat ccgttttagg aaaaacgtac
    11161 ggattgacga cgagtggacc tgttgcagaa attattagca gatcctatgt catttcccca
    11221 agctataaga cagacttaac cgggataaat cgtacattag ctgcaaatct acctcggacg
    11281 acttacttat gtaatcagtt cttggagcat ttaccaccgc tctcagaaaa ggacgagaac
    11341 tttctaagac tgcttattgt cgtgacttta tctgattatg tagatgaatc catcgagctg
    11401 aagggcctgt tgcttgctca cggagatagt acggctagca gcattcaagc agtagtgaat
    11461 caattatgcg gcaattatgt ttttgaagcg ttggatatgc cagtagagag taatgtgtca
    11521 gagattgtcg aatcgacaag gaatttcgtg aaacggcaag tgaatacaga tagtctagtg
    11581 ctgatcgtag atatgggctc tttaaatcaa atttacacac agatcaagaa cgatttaaaa
    11641 ggggagttac ttcttctgaa taatttgacg acttccattg cacttgatat tggcttgaag
    11701 atgacgatga atgcgccatt taaacagatt gcggaggatg cagcagacaa gtattcaatc
    11761 aatgttcaat actttgaagg cttttctcaa agtagcaaca ttatcatttc ctgtatgtcg
    11821 ggtttgggga tttcggacaa attgaaagat gtttttcgtc atcatttatc ggatgaatcc
    11881 atcgaagttt tcacgaagga ctacagagaa ctacgtgaat taatcgacca aaatgatgaa
    11941 acttattttg acaagacaaa attggtcatc acgacttcgg atcttccacg ttccttttca
    12001 attcctaata ttaatgttta tgacatctta gacggtgatg gagcagccaa tttggagcat
    12061 attttgagtc ctgaaatgaa gccggcagaa tttaaccgct tgattcagga gctagtaaaa
    12121 ttcttttcgt tggaaggaat tgcggaccgt ttgaactttc tgaacccaac gatcgtgatg
    12181 agtgaagtgg aaacagtcat ttctaaatat gaaaattact atcatttcac tttaaatggc
    12241 cgtgtaaaac taaatctcta tatgcatacg gcattgatga tggaacgttt attcttggct
    12301 cggacagaaa aatctgaaga accgaattat gatttgaaac cagatgaaga tgaattttat
    12361 tatgtttcta gaagtatttt tcaatcaatg gagatgaaat ataattttaa agtcaatgct
    12421 tatgagcttt cgctgctcta tgaattatta ggaccgtata ttcaaaaata aagtgtctaa
    12481 aaaagtgtct aatctgtttt ttttaggtta gacacttttt ttaatgtgaa ttaacgtgat
    12541 taaaccaatg tttttaaaag ttggcacagg aattgcttat ataaaagtga acagaaagaa
    12601 aggaattaac catatgaaga aaattctaat agcgactcac ggtcacttag cttcaggctt
    12661 tcttagcagt attcaattac tgacggggaa ggttcaagag attacggtga ttaatgctta
    12721 tgtggatgag tgtgattttg aacaggagtt gaatgtattt gtaaaccagg tgaacgagca
    12781 ggatcagatt tttgttttta ccgatttatt tggcggaagt gtcaatcaga agattaccaa
    12841 aacctttttg gagaaagaga ttgctgtaac tattttgtct ggctttaatc tgccaattct
    12901 gctcgaaata gtattggcaa ccaaggaatt aacaaaggag gatattcagg aactggtaaa
    12961 taaatgccaa caggaattaa agatgaccat tcttgatgag gtacatgtag taaatgaaga
    13021 ggaagatttt ttttaggagg aagaaaaatg attaaagcag tgcgaattga tgaacgatta
    13081 attcatggac aagtagcgat gacttggaca aagagtctaa atttaaccgg gttagtagta
    13141 gcaagcgatg aggcggctac caacgatatt caaaagatga cattgaagat ggctgcacca
    13201 tcgaatgtca aagtcattat taaaacagtt gatgacgcga ttcatttgtt aaaagatccg
    13261 cgggcgaagg acatgcgctt gatggtttta gtccctaatg tcaaagatgc ggtaaaagtt
    13321 gcgaaagctt tcccaacgga aatcgaatta atgaacgtgg ggaatgctgg gaaaatgaca
    13381 ccgccatcgg aagataagaa aaccttatca aaggaagtga tgttaacgac gtcagaaatt
    13441 caagacttaa aagaattagt tgaactttat ccagataccg tttttcagcc tacaccggcg
    13501 atggaaaaac gttcagctat ctctattttg agtgattttt agaaaaagga aggagaaaaa
    13561 ttatgtatga tgcatttgta gtcgctttgg ccgtttttat tggaattgct ggacatgaaa
    13621 tgttcgggat gtccatgctg agtcgtccga ttgttgttgc tccgttagca ggagcgttga
    13681 tgggggattt gcagacgggg ttgattgtcg gggcgtcact agaagccatc tttatgggtg
    13741 tcgtcaatat cggaatctct agtacggcgg agccttcttt ggctgctggg ctggctacag
    13801 cgttcgcaat caaaatggga ggaaacgtgg atgcgattat tcccattgtc tttccactag
    13861 cagtattagg attacagtta atgaatatga ttttttcttt tgtatgtgga ccgatggctg
    13921 ctaaattttt caaatttgca aaatctggtg atcaaaaagg aatcatcaat ctgcacttct
    13981 ttatgtggtt tgtccactat gcgctttatg ctcttattcc attctttgcc gttttatttg
    14041 gagcagatgt tgtcaaaagc gtcttggata atattccaga ggtcatcatg aacggattaa
    14101 cagtcgctgg aaatctgctg ccggcagttg ggatggcgat gctgttacgg atgctttgga
    14161 gtaaaaatat tgctgtgtgg tttttctttg gggccgttgt tatggcgtat ctcgagttac
    14221 cattgattgc aattgccgca gttggaacga ttattgcaat tgttatagcc caaagagatc
    14281 tacaactaaa aaatatatct gttcatgcag ttcaaagcga tgctgctgta aatgcaacgc
    14341 ttgaccaaga agaggaggat ttctttcaat gaaattagct gatggaattt ctaaagatga
    14401 gaaaaaattg gttcggaaga tgttttggcg ctcgatgact atgtatgctt cggtgaatcc
    14461 ggtgacgatg ggcggcggtg gtttcgccta ttctatgatg cccttcatta atcatttcta
    14521 caaaagtgaa gaagatcgag ctgcggcgct ggaacgtcat acacagtatt tttcgacgac
    14581 cattccttgc gcgtcctttg tcatggggat tgcggcatcg atggagaagg aaaattctga
    14641 aaaagctggt tttgacgtgg aaagtatcaa cgcgatcaaa acgagtttga tgggaccatt
    14701 ggctggaatt ggcgattcat tgttctgggg agtttggcgt gtcgttaccg cgggactagc
    14761 gatcaactta gccaatgccg gaaatatttt ggcaccaatt atctttttag ttatgttcaa
    14821 tatacctaat tacttgatgc gatactatgg cggcgtttta ggctattcat taggctctaa
    14881 atatattgaa aagctgtatg cgaacgggtt aattgaaatt ctgacaaaag ctgctagtat
    14941 cttgggtctg atcatggtag gtgcaatgac gatcagtatg gtcaaattca ataccgtttt
    15001 gagcttttca atgaagggcg aagaactaat gaatgtccaa gaaatcctcg atagtatttt
    15061 taaaggaatt gtaccgttat tggtaacatt gagctgtttc aaattactag ataaaaaagt
    15121 cggcgtcatc aaaattattt ttggaatggt cgtcttggga attttattgt cattacttgg
    15181 aatcgcatag taggaagctg cagctactag gctgcagttt ttttagcaag gagggaaaag
    15241 atgactatta atgaagtaat ttattcaccc aaacacccat tttttaatgc tgagttaaac
    15301 gacggaaatg tgactacttc aaacgaatac agctactatc ggaatattcc tgatgtaaag
    15361 ggattgacct tcgatgtaag taatcaaaag acattagcaa accttgatgt ttgcgggacg
    15421 attcaaacgt tgacctttta tcgagaaaat ttactaactg aggaaaaacc aggtgtttgg
    15481 gtcaataaac agctctctca aaccaattcg ttatccttaa aggttgaagt aaacgggatt
    15541 gtctaccatt tgtcagaatc ggatcatcgt attgaagtcg atgtgattca agactgtttg
    15601 cctagatata ttcatcatta ttcgacgttt aaggtcattg tagtatcctt tgctcccata
    15661 ctggctaata agcgtttaag tatgcttgtt caacaggttt acattgtgaa tgaaacacac
    15721 gaaagccttc aagtttcagt gaaagatgtg ccttcgtatc aagagaagta ttcggatcag
    15781 caaaatgtgt tgatttctca aaaaggaaat aaggacacga tcgcacaaca ggaagtggca
    15841 agcttcgcca ttgcttttgt ggacccaaat gcttttgaag aagtaatgac ttttcaggaa
    15901 tctgatatcg aaaaatggct gaaggaaaca ttgaactatt ttgctcagct atttgggcag
    15961 ctgtcaatgc caaacgatag agttgtccat ctctatcgtc gtgcacttta tcaaagcttt
    16021 tctagtttcg gtatgaatcg acagggacag gtcgtcggca gcaattgggg cagctatcct
    16081 gctactaatc gaatttggaa taaggatatg tattacgcat cattgccatt caccatgttt
    16141 gaaccggagt tatgtcaaaa aacggttcta tggtttgatc aatatggcgt taagtttcct
    16201 ggttcaaaat ttacaggagg tatcaatcat tctctgagta attcgttatc cagtgcgtta
    16261 ctggctagtc tttattttga acatactggg gatacaaaat tctttgagcg gtgtccgcag
    16321 gttctactga atgcttcaag aattattgag gacatattag ctcaacgtcg gtcaggggaa
    16381 ccaatgcttt ttccatctgt ttggttatca gacgcctttg cacttggaaa gtatcatacc
    16441 ggcagcaatc tttgtctttg gaaggcctgt attggacttg cagaaatttt tgaagcccta
    16501 gaacaactga gtcttagcaa gcgatacaaa aacattgcct gtaaactaaa ggaatcaatt
    16561 tgtaaacaaa tgactatcga tggtcctttt ggtgagcaat ttttggaggg aatcggtgat
    16621 gaggagaaaa caacctattc agtgaagcac tatcaaaaac cgattcttga gcaagggtta
    16681 atctttttaa gtgatgtgat tgtggatgga aaaattaatt tattgatgca tgatggagaa
    16741 gaatcagaca ctacgttgat gcctttttat caatttttag ataataaaga tcatttatat
    16801 cagaacacca tggccttttc tgctagttct ttcaatccaa cctatagcga ggaaatcaaa
    16861 ggcatcactt ggggattaga atccggcgcc acttttcctg gctttatcac tgttttaatg
    16921 agcgagctgc acaaccctca agctttcgta acccgattag aagaactaat ttgtttggca
    16981 gatttagacg gttcgtggtg gtggtggccg tatcgcttag aggctcaaca aggagaagtc
    17041 gtgcgagact ttggctgcgg caaatgcggc tgggcatcgg gtctttttgt cagcctaatg
    17101 atcagtcaat atttcggatt gaaattcaaa aaaggagtat tgcaaattga tcctgttgat
    17161 aatttaacgt tttcttggaa gaatttgaag ttcggtcaag ccttcatttc gattgaatgt
    17221 acgcaatccg agttgagtat ttcaaatttg tcagaaaagc cattgaaaat aagtgatacg
    17281 aaaaaaattc ttcacgtaga aaagggaaaa acgattcaca ttcaaaggag aaaaagatga
    17341 gtatactaaa aaatattacc caatttaaaa aaatccagag tcgagcaatc actcccgaaa
    17401 atccaacagg cgagaaaggt aaagctgcga tgttgccaag cgagctagga ccaagccgca
    17461 aaggtcgtgg cgcaattccg ctgacttctg gtgaagaaac tgtgattgcg gatattactg
    17521 gaacgggaat aattcgtcat atgtggatga cgattcgtga tcgaacagaa tttggcagtt
    17581 ttgttttacg tgatttaatc ttaagaattt attgggatgg gtcggatagc cctgctgttg
    17641 aaagtccttt aggggatttc ttttgtaatg ggtttggaga gcgttgcgag gtgaactcgt
    17701 tgccgatagt ggttaatcct acagggggct ttaattccta tttcgaaatg cctttccaaa
    17761 catccgctaa aattacgatc gagaatttac atccgaagga tatgtcctca tttttttata
    17821 cgataaatta cgctttggtg gacgaattgg aagaaaatag cctatatttt cacgcaaatt
    17881 ggcatcggga acgcattacc aaattacagg aggattataa gttaattgat cgattaaagg
    17941 gtcaaggcta ttacgtgggt acttaccttg cgcttacctg cctagaacgt tattggtggg
    18001 gcgaaggcga gtttaaattc tacattgatg gggatagtga gtatccgacc gtaacctcaa
    18061 caggttcaga agattatttt ggcggggcgt gggcctttca tgagtacgat gagttcggac
    18121 gtccacatgc gaaaaactat tcaacagcct ttttagggta tccttaccaa tcaaatagag
    18181 atgccacaag agatcgtttt gaaacgggaa aattgaatgc cgttcatgcc tttggtgacg
    18241 acgcaatgcc acgacatggt ctttatcgtt ggcatcttca agaccccatc tgttttaccg
    18301 aagatttatc ggtcacttta caacaaatcg gcaacgatga tgttcgctta tttgagcggc
    18361 aagacgatgt tgctagtgtc gcttactggt attcaacaga agcttcagga aatactaaac
    18421 gtcatttcac tgatcgagaa cgcttgccgc gttaatgaag tgtttttcac atattcgaag
    18481 ggaggaacct atgatgatat atggggcaat tgaagctgga gggactaaat ttgtctgtgc
    18541 agtcggtact gaggaattaa cgattttgga aagagtcagt tttcccacaa caactccagt
    18601 tgaaacaatg aagcaggtca ttgatttttt taaacctttt aaggaacaat tggcaggcat
    18661 cggggtcggc tgctttggtc caattgatat tcaatgcaaa tcaacgagtt tcggtcatat
    18721 cacatcaaca ccaaaacttg cttggcaaaa tttcaatttt gtaggaacgc taaagcaata
    18781 tttcaatatt ccgattgaat ggacaacaga tgtcaatgcg gcttgctatg gtgaatatgt
    18841 tgctggttct ggcaaagggt tagcaggtgt tgcatacttc actgtgggaa caggtatcgg
    18901 aggtggtgcc ttgttcaatg ggactttcgt ggaagggttc agtcatccgg aaatggggca
    18961 tatgttgatc aagcgacatc caaaggacaa tttttcagga aactgtccct tccaccaaga
    19021 ttgtttagag gggatggcag caggaccagc gattgaaaaa cggttgggtg taaaaggcca
    19081 gaatttattg gctgacgatt cgttttggca gattgaagcc ttttaccttg cccaatgtgc
    19141 atacaataca acactgatgt tttcgccaga tagaattatt tttggcggtg gtgtaatgaa
    19201 acaagaacac atgaagaaaa aagttcagga taaatttgtt gagcttataa atggatatgt
    19261 tgaaatacca ccaattgata gctacattat tacgcccgaa ttaggtgata atgccggaat
    19321 aatcggcgga ttagcattgg ccagaaaagc ggtaagaaat aagcaaccat aattaatgaa
    19381 tgtttcattg atactcatgt caaaagtatt taaccgagta atcaaatgat caaatgaagt
    19441 tggataaagc gaaaatgaat tcaaataaaa aggctctaag tcaaatcgtg ttgttttcat
    19501 taaatcactg aaaggaaatg cttctgcgtt tccttttttc tttttgttgt gcgctgtata
    19561 ccttgaggat attcactggt ggcgtacact taaatagtaa tatattgtgt tactttatgt
    19621 ctgtggtaat agttatagtt gcttaccaat gtatgttcat ttttcgaaat gttgacttca
    19681 taatccttgg cttaatgttt gaattttttt ggtgatagat tttttcagcc gtataaaata
    19741 aatcgtaatt ctattataga gagttgtgat tataaaaaga tagcaagaaa aaaaaagaaa
    19801 gtttctatac attttaaata ttttattcct atttctattg ctaaaaaagt agaaattcta
    19861 tatttacctt taactctcgc ttctcttgca aatgtagtta gcattttaat cgataataaa
    19921 ctcaggtagt tatattacct tgaaatgatt gataattttg tcacttctat tttttttgca
    19981 ataaattttt tattttagtg aaaaataaga aactatttct ttgaaggcga taagttagaa
    20041 aaatattatt gtagtctttt ctttattagg aatcatcact gttgtatctt attacctttt
    20101 ctgtttttct tattagagaa aaataatttg agaggtagat agtttttatt ggaggaagta
    20161 attatgtata tttttgaaac aaaaattttt cattgcaata attgcaacaa agaaatttca
    20221 tcgggggaag aggtttggac aaaaatggaa atttccgcca aaagtaactg ctcctcaact
    20281 gaaatcaatt aaagaactag aatttgagaa tgcactggta atttgtatga catgtactga
    20341 tgaagtattt cgaaaacatt tttaaaggtt gagatgtata tagatttaac caatcattta
    20401 tctctctcgg aaaaggaata ttaactaacc agcgggttat caaaaatagg taggaaaact
    20461 gtacttaatg gcttatttcg cgctgattat gagaatatca gtctgtttga ttaattttta
    20521 gggttcaaaa tacagagaca aactccaggt cgggtttcgc cacaagcgaa ttacccgatc
    20581 tggagttttg tctattgcag gaaggcgtag ccggactgca ggaataaagc ccccggtt
//
LOCUS       DACEEZ010000048        20595 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20313_2017
            SAMN12639966-rid9879223.denovo.048, whole genome shotgun sequence.
ACCESSION   DACEEZ010000048 DACEEZ010000000
VERSION     DACEEZ010000048.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639966
            Sequence Read Archive: SRR10023158
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 20595)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20595)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741683.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741683.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:44:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,016
            CDSs (total)                      :: 2,962
            Genes (coding)                    :: 2,889
            CDSs (with protein)               :: 2,889
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 36 of 73
            Pseudo Genes (incomplete)         :: 29 of 73
            Pseudo Genes (internal stop)      :: 18 of 73
            Pseudo Genes (multiple problems)  :: 8 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20595
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639966-rid9879223.denovo.048"
                     /isolate="E20313_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            99..293
                     /locus_tag="IWO89_001746"
     CDS             99..293
                     /locus_tag="IWO89_001746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0456493.1"
                     /translation="MYYVEVKTKGVKNKQYVKGISNEYPLLGSWKEAAPFSKPCAIKI
                     KNELEKELTCGKAVVDIIEK"
     gene            562..861
                     /locus_tag="IWO89_001747"
     CDS             562..861
                     /locus_tag="IWO89_001747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0456494.1"
                     /translation="MDYTVEFDSELLKQFPQEEHFMYKEAISHKVTEYLEHNFFRIKP
                     VRTRSAKGIYEMKVKINKKNYRIAFALKKNHVFIFYISSNLQKIVFDKEVSKKIK"
     gene            complement(958..1392)
                     /locus_tag="IWO89_001748"
     CDS             complement(958..1392)
                     /locus_tag="IWO89_001748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAQ0456495.1"
                     /translation="MNIDSLMIDEISELYKSVGWTNYTKDTARLARAFEQSESLIKRN
                     GEGKIIGIVRWITDFATIAFIQDILIHPRYQRQGIGKVLLNEALEKITSYGPVQIELL
                     TDDTEKTKKFYESVGFVQVKEMGGISYVIDTRRKKVLFGNDK"
     gene            complement(1624..2334)
                     /locus_tag="IWO89_001749"
     CDS             complement(1624..2334)
                     /locus_tag="IWO89_001749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010780519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase"
                     /protein_id="HAQ0456496.1"
                     /translation="MSCEEKIAAMKQRVLEANLALPRYGLVKLTWGNVSEVDRELGVI
                     VIKPSGVRYECMQADQMVVTDLSGNIIEEDSLKPSSDLPTHVVLYQTFEDITAITHTH
                     STHSVMWAQAGRDLPAYGTTHADAFYGKVPCTRQLTKEEVREAYEVHTGNVIVETFKE
                     RKLDPNEVPGVLVYGHGPFTWGDSPMKAVENSLILDEICLMAKENELINPNICEIPQY
                     LLDKHYFRKHGKYAYYGQ"
     gene            complement(2324..3184)
                     /locus_tag="IWO89_001750"
     CDS             complement(2324..3184)
                     /locus_tag="IWO89_001750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 3-epimerase"
                     /protein_id="HAQ0456497.1"
                     /translation="MAQIGIYEKALPKDISWKERFSLVKEMDFDFIEMSIDETDERLA
                     RLDWSEEKIAELREEMFSSGVRIHSICLSAHRRFPFGSADPEKKKAAKQLMKKAIHLA
                     HNLSVKVIQIAGYDVYYEEKSMNSREDFIEGIKESVKEASKYGIILSVEIMDDPFMNS
                     ISKFLEIKEQIHSPFLQVYPDLGNLSAWPENNPARELEKGIDYITAIHLKDTYPVTKE
                     YSGQFRDVPFGSGCVDFLGLLRHLKRLDYDGTFLIEMWSEKSQDFRREIQQAKDYLSS
                     KLKEAGYDVL"
     gene            complement(3189..3827)
                     /locus_tag="IWO89_001751"
     CDS             complement(3189..3827)
                     /locus_tag="IWO89_001751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010740138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-keto-L-gulonate-6-phosphate decarboxylase
                     UlaD"
                     /protein_id="HAQ0456498.1"
                     /translation="MSRPNLQIALDHNDLEHALGDVMKVGDVVDIIEVGTILCLQEGQ
                     KAIRCIRSMFPDKKLVADTKCADAGGTVASNVAKAGADWMTVICCATIPTMEAAQKEI
                     GELQVELYGNWTFEQAMDWHNIGIRQVIYHQSRDALLSGETWGEKDLSKIKKLIELGF
                     NVSVTGGLNPHTLHLFEGIDVYTFITGRGITAANDPMKAAQNFKDEIIRIWG"
     gene            complement(3842..4141)
                     /locus_tag="IWO89_001752"
     CDS             complement(3842..4141)
                     /locus_tag="IWO89_001752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAQ0456499.1"
                     /translation="MRILVSCANGSGTSLMMMRSVEKAMKELNVPITKIHHCAISEGK
                     SSAGQYDVVFTPVNFLNMFTQAEKKGVTVIGIKNVMSVKEISEKFAETKWYQKNK"
     gene            complement(4184..5668)
                     /locus_tag="IWO89_001753"
     CDS             complement(4184..5668)
                     /locus_tag="IWO89_001753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIC"
                     /protein_id="HAQ0456500.1"
                     /translation="MGEFLLSIWTYFATNILTQPAYLIGFIVLLGYILLKRPLHECIA
                     GFLKATVGYFILTVGSGGLVNNFRPILVGLKERFNLDAMVTDPYFGQNAVDAGLMETF
                     GRTFGDVMILLLIAFVLNILLVRFQKYTKLRSVFTTGNVQVQQAATAFWILLFCFPDL
                     GRIQALIFMGLILGCYWTVASNLTVGITQELTEGAGFAVAHQQMFGIFIFAKLAEWMK
                     KRDEKKNRSIKQDKKIEDIKLPGFLSIFNENMVATSLLMLFFFGIILIVLGKDYLIQA
                     QFMQEGQSFLFYIMTTSLNFAVYLAILQLGVRTFVDELTQSFQGISNTILPGAVPGID
                     VAATFGFGSPNAVTIGFLFGALGQFLMIGLLILLKSPTIVIAGFIPLFFDNAVIAVYA
                     NNRGGFKAACLFPFLSGIIQVGGSALFATWIGMSTYGGYIGMFDWATAWPLFTVIMKV
                     LGYAGIAVVVIGLLAIPQLQYRKNPDGYFLIVEDYDTYAEKQRV"
     gene            complement(5688..6149)
                     /locus_tag="IWO89_001754"
     CDS             complement(5688..6149)
                     /locus_tag="IWO89_001754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HAQ0456501.1"
                     /translation="MLKYFFDHQLIHYPDKEITDWREAIAESCLVLIDKEIIDETYVE
                     EIIHCVEKYGPYIVIAPQVAMPHSSEKSDGVFGTAISFTKLKQPIRFESEGENEEKTA
                     SLFFTLAAKDPEEHLENIQKLSELLMTDGLVERLISTNSIDDYQVVMAEYN"
     gene            complement(6167..7234)
                     /gene="ulaG"
                     /locus_tag="IWO89_001755"
     CDS             complement(6167..7234)
                     /gene="ulaG"
                     /locus_tag="IWO89_001755"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ascorbate 6-phosphate lactonase"
                     /protein_id="HAQ0456502.1"
                     /translation="MSKTVNEVTKEKWLVETFPEWGTYLNEEIETEVVDPGTFAMWWL
                     GCTGIWLKSHEGTNILCDLWCGTGKRSHGNGMMKKGHQMMRMSGVQNMQPNLRTQPFV
                     IDPFAIKNVDALIVTHIHSDHLDIHTAAAVHQNCPEATFIGPKEVVRTWQKWGIPEEK
                     TITVKPGDAVKIKDINVVALEAFDRTALVTCEDPEEVLKGKMPQEMDQIAVNYLFETS
                     GGNLYHAGDSHYSNLFAKHGNEHKIDVCLGAYGENPRGITDKVTSVDMLRMAESLNAK
                     VVIPVHYDIWSNFMADPKEITEIWKFKKDRLKYGFKPYIWQVGGKFIYPTNKDDLEFN
                     FYRGFEDVFSIENDTPFPSFL"
     gene            7452..8222
                     /locus_tag="IWO89_001756"
     CDS             7452..8222
                     /locus_tag="IWO89_001756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HAQ0456503.1"
                     /translation="MKNSLVNIEKRQKEILELLQQTEQLSTFELAESLDVSISTIRRD
                     LNLLEEKNEIIRNYGYCSYNYKNQTDFDQSGPERIKQAIAREASKYINDYDTLFINSS
                     STALKTLDFLQSNHLTVITNNVKINYSPHKDNCSYILTGGEMRFPKEALVGDIALNTI
                     SSVNADICIIGCSGVDVVHGVTTKILNESTINEMMVNKTIKCKILVADHRKIGLTSKF
                     KIADISVFDYLITDKFCSEKVLEEIRNTGIKTIQVDSF"
     gene            complement(8495..9322)
                     /locus_tag="IWO89_001757"
     CDS             complement(8495..9322)
                     /locus_tag="IWO89_001757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAQ0456504.1"
                     /translation="MIKVDGALLKEYRKKKGLTQVQLAEGICEQATVSNIENKNMSPG
                     INILSALCKKLNVEISDILVETEEDKIRKKINEIQSLNSVSKYQESYKILQTIDQEKV
                     SNSDLLNQILFYQGLLDHLYAKKPDAALFYFNQVLEQTTETDIHHLQAAANVAMIYLS
                     KGELDFAKVYVERTLKILSETDFDNLMVCIVYYDIATYYRKIEDYDKAIQLCEKGIEY
                     NKKHKSTYALEYLLYEIASCHKQLGEDDYLERYMDAKKIARFNGNDYAVKVIENDLK"
     gene            9890..11125
                     /locus_tag="IWO89_001758"
     CDS             9890..11125
                     /locus_tag="IWO89_001758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl transferase"
                     /protein_id="HAQ0456505.1"
                     /translation="MKTILFAPAVFNLAETTRMIEVAKAMKDRYYCVFFGFSKTFAPL
                     ITESGFEYHLLAPILTKKQEEQIIKFDQLRTLKNPFTKEMVASRITSEQSLIAKFEPR
                     LIVTGSNVTIFLSARNKKIPLVYVKPYALSEPFFQSEHALMPHELRFFGPLSNPLWHW
                     TKKRIVNTRWIPNAFKHAAQKEMLPLPKTSLQMMDADLNLITTPKLFLEEEQLPENYR
                     IVGPIFAKLSTSLPEEVLTFIQRKQQERKRIIYFAMGSSGNPRMLRKILFFLQKQPDL
                     AVIAPIDYLLDDIQSDSEDLLITEYLSSHLLHEFIDFSFIHGGEGTVQTACESGKPFI
                     GVGMQFEQSCNIDYCVKYGNAIALTKPVTERKLQIALREVSSPKIRQQARTLQAVFPI
                     NGPQQAIQEIETFLSNHPI"
     gene            complement(12124..12384)
                     /locus_tag="IWO89_001759"
     CDS             complement(12124..12384)
                     /locus_tag="IWO89_001759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HAQ0456506.1"
                     /translation="MYWLILFFVFIFLLTASHLILNMLATYHIQINRWIWALASFLIV
                     ILPKIIVPHMNVLFSWGTYVLCGIFAINFMIEQHRWFVTSKL"
     gene            complement(12411..13712)
                     /locus_tag="IWO89_001760"
     CDS             complement(12411..13712)
                     /locus_tag="IWO89_001760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAQ0456507.1"
                     /translation="MEKLMKWVEEKLVPPMAKIGTQRHLLAIRNGVVSTLSLILIGTF
                     FMVFINLPFPGWNEFIAPYSATIVLPFRMTMGLMAIYATFVMGSDLAKSYGLDSVTGG
                     ILSLGTFFMLQVPVNVLTPEEAPLGWVLPMSSLGASGMFAGILSMIFAVEVYRFFKNR
                     NITIKMPEQVPPAVARSFEALIPGAVILTITWLIRSVAGFDVNAALLSLFEPLTNILG
                     NNLLGVLLPMFLIHLLWSFGIHGMSIVGAVVRPMWLIMLDENAKALADGTAATQLPYI
                     APEQFYQWTVTIGGAGATIVVSVLFIFFCKSKFLKEVGRFSIIPGIFNINEPIIFGAP
                     MMLNPYMFIPFNLVPLVLTIISYFAVKLEMVNRFTVLPTWTLPAPIGGYLSAGNDWRV
                     VILIMINTLIAFIIYYPFVKAYDKKMLSDEQSRGENEVQTV"
     gene            complement(14004..14462)
                     /locus_tag="IWO89_001761"
     CDS             complement(14004..14462)
                     /locus_tag="IWO89_001761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HAQ0456508.1"
                     /translation="MHFFKTRNILKAVASGQIIPLTAVKDEVFSSKMIGVGYAITNHD
                     GYIHAPVKGIIDNIFPTRHAITIESESGRKILIHMGLDTVELEGSPFQIRVESNQEVK
                     EGTLLAIIDLKKLEQAQKDTVIIVVSPGIFEGNLLKQNQEVKIKDSCFEF"
     gene            complement(14475..15800)
                     /locus_tag="IWO89_001762"
     CDS             complement(14475..15800)
                     /locus_tag="IWO89_001762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005227470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltose-6'-phosphate glucosidase"
                     /protein_id="HAQ0456509.1"
                     /translation="MKKQIITIAGGGSTYTPGIVQAILNNEKRLPLSEIRLYDIDEER
                     NMDMYLIVKFMLKRKGFSNIRIRATDDPKLAFTGCDFVFSQIRVGGLEMREKDEKIPL
                     KHGLVGQETCGLGGFAYGMRSIKGLLEIVDHIQDYAPKAWILNYTNPESIVSEAVRRK
                     YPNVKMINACDMTISIEETIAVNYGYDRKNWIVTYYGLNHFGWYTSIYDKELQREIMP
                     EIIEKLITKEMQVADFNIGDKTWQKTFQMMSVITKNFPSNIPNNYLEYYLYPDMVVEH
                     TDKEYTRANMVMDGREKNTKEMADKIRRGIVEEVLNFNFGEHGQYIVDIAISLLNDDR
                     RRFMLIVPNQGAIPNLRSDAVVEIPAYVGATGVEPITLRKPISDFHKGLMEAQVAVEK
                     LLVDAYFEGSYQKALQAFTLNQTVPNARVAKKILDEMIKVNKKYWPALK"
     gene            complement(15817..17229)
                     /locus_tag="IWO89_001763"
     CDS             complement(15817..17229)
                     /locus_tag="IWO89_001763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAQ0456510.1"
                     /translation="MNVKSNVQKLGRAMLLPVAAMPLAGLIMRLSADDMLNIPVIGAA
                     GNAVFGNLDILFAIGVAIGFVKNKDKGIPALTGFLAIATLKKGLEIMDPEVNMGIFGG
                     IISGLVAAWTYNRFKEQKLPVVFSYFAGEKFPLTMVMILQTIAAVFLGFIWPLIQIGI
                     DNFAKLLVDMGALGVGIFMFLNRLLIPFGLHHVLNTYIYYDLGSYTSPSKEVFRGEMT
                     RFINGDPQAGLFLSVFFVVMMFGIPAICLAIYKAAYKENKEMVKGIMGSGAGTSFIAN
                     ITEPVEFSFMFLSPMLYVIHALFAGLAGIVCYLFNIRIGFTFGACIVDYLINFRIATN
                     AILILPIGIFFFALYYVTFYYLINKRNIQTLGREAKAEFGNEVTLEETELGLASKNYE
                     YMATKMLQAFGGKANILDVYSCNTRLRVEVVDPTMVEEQRIKQLGISGIIKPTEKNYQ
                     IIIGLEVTYVMAEFNKLLEE"
     gene            complement(17246..18010)
                     /locus_tag="IWO89_001764"
     CDS             complement(17246..18010)
                     /locus_tag="IWO89_001764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate deacetylase"
                     /protein_id="HAQ0456511.1"
                     /translation="MGKVIFNSDDFGYSHGVNYGIMDAYQRGILTSTTLMANMPGFEH
                     AVKLRKEMPRLGVGVHLTLTCGKPLLKTVDTLIESGEFRSLSYYQHPFNIDKDQLYQE
                     WNAQIQKIYHAGIIPTHLDSHHHTHTLDENQEVVVTLAKKYDLPVRSNFERKDEVRHV
                     DYFEPCFDVVGETDEKKRQINTSVEEYLASLLMILREDKTIEVMCHAAYLDQELLNGS
                     SFVYPRTHQVEFLIHSAFAQKVKNDPLIELVTYEDI"
     gene            complement(18114..18989)
                     /locus_tag="IWO89_001765"
     CDS             complement(18114..18989)
                     /locus_tag="IWO89_001765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAQ0456512.1"
                     /translation="MKESASCFVLSYRGGELTVYVMKKLNHNAVLIDDDGIEKIAMGK
                     GIGFSAKVSKPFDVEDADKLFVLDSKEKIKRFSEIVSQIPIEFMDFAEEVIELITARI
                     SSPLDSNIYITLTDHIYFAIQRKREDRDVTAIMLPEMRFLYPNEYDIAVEVVERINHR
                     YQTELGSNEVGFITMHIINAELGEPNSLKSLKIVEMTTTILSHLEKSYSNVYDKESLT
                     YHRLMVHIKFLVKRLIYQEETPVENIGFFTETFKHSKAYCTALKIKDIIEQQFQLAIQ
                     ENEIIYLAIHLARIY"
     gene            complement(19244..19328)
                     /locus_tag="IWO89_001766"
     tRNA            complement(19244..19328)
                     /locus_tag="IWO89_001766"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(19292..19294),aa:Leu,seq:caa)
     gene            complement(19346..19417)
                     /locus_tag="IWO89_001767"
     tRNA            complement(19346..19417)
                     /locus_tag="IWO89_001767"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(19383..19385),aa:Gln,seq:ttg)
     gene            complement(19427..19499)
                     /locus_tag="IWO89_001768"
     tRNA            complement(19427..19499)
                     /locus_tag="IWO89_001768"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(19464..19466),aa:His,seq:gtg)
     gene            complement(19561..19645)
                     /locus_tag="IWO89_001769"
     tRNA            complement(19561..19645)
                     /locus_tag="IWO89_001769"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(19609..19611),aa:Leu,seq:caa)
     gene            complement(19666..19736)
                     /locus_tag="IWO89_001770"
     tRNA            complement(19666..19736)
                     /locus_tag="IWO89_001770"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(19702..19704),aa:Cys,seq:gca)
     gene            complement(19782..19853)
                     /locus_tag="IWO89_001771"
     tRNA            complement(19782..19853)
                     /locus_tag="IWO89_001771"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(19819..19821),aa:Gln,seq:ttg)
     gene            complement(19865..19937)
                     /locus_tag="IWO89_001772"
     tRNA            complement(19865..19937)
                     /locus_tag="IWO89_001772"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(19902..19904),aa:His,seq:gtg)
     gene            complement(19940..20013)
                     /locus_tag="IWO89_001773"
     tRNA            complement(19940..20013)
                     /locus_tag="IWO89_001773"
                     /product="tRNA-Trp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(19979..19981),aa:Trp,seq:cca)
     gene            complement(20020..20100)
                     /locus_tag="IWO89_001774"
     tRNA            complement(20020..20100)
                     /locus_tag="IWO89_001774"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(20064..20066),aa:Tyr,seq:gta)
     gene            complement(20102..20177)
                     /locus_tag="IWO89_001775"
     tRNA            complement(20102..20177)
                     /locus_tag="IWO89_001775"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(20142..20144),aa:Phe,seq:gaa)
     gene            complement(20213..20285)
                     /locus_tag="IWO89_001776"
     tRNA            complement(20213..20285)
                     /locus_tag="IWO89_001776"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(20250..20252),aa:Asp,seq:gtc)
     gene            complement(20295..20367)
                     /locus_tag="IWO89_001777"
     tRNA            complement(20295..20367)
                     /locus_tag="IWO89_001777"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(20332..20334),aa:Val,seq:tac)
     gene            complement(20383..20454)
                     /locus_tag="IWO89_001778"
     tRNA            complement(20383..20454)
                     /locus_tag="IWO89_001778"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(20419..20421),aa:Glu,seq:ttc)
     gene            complement(20469..20558)
                     /locus_tag="IWO89_001779"
     tRNA            complement(20469..20558)
                     /locus_tag="IWO89_001779"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(20522..20524),aa:Ser,seq:gga)
BASE COUNT         6501 a         4312 c         3269 g         6513 t
ORIGIN      
        1 tgattctttt tatttatcat aaagatatag atacataatc attttcactt acatatattt
       61 gaatattaac taaaaacagc aagaaagaag aggatcaaat gtattatgta gaagtaaaaa
      121 caaaaggcgt caaaaacaaa caatatgtga aaggtatcag taacgaatat cctttacttg
      181 gttcatggaa agaagctgct cctttttcca aaccatgtgc cataaaaata aagaacgagc
      241 tagaaaaaga actgacttgt ggtaaagctg ttgtagatat catcgaaaag taaatccaat
      301 gtatagaaag gaagatcaca catgaaaagc gacgtacaaa ttgctaagat tatagaatta
      361 gaagaagaag acgcagtgtt gacaattgaa aaaaaataaa tgtttgtata ccatatgatt
      421 atttcaatat gtatcctgga attggagatc tcgtagagct atatcaagat gaatcaaata
      481 tacttgtcat tcatcatgaa atatagagac ttataaaatt gtttattttt ataatgtatc
      541 agtatagaaa ggaaataaaa catggattat actgttgaat ttgacagtga actacttaag
      601 caatttccac aagaagaaca tttcatgtat aaagaagcaa tttcccacaa agtgacagag
      661 tatttggagc ataatttttt tcgtataaag ccagtgcgta ctcgttcggc aaaaggaata
      721 tacgagatga aagtcaaaat taataaaaaa aattatcgca tagcatttgc tttaaaaaaa
      781 aatcatgtgt tcatttttta tattagttcg aatttgcaaa aaattgtatt tgataaagag
      841 gtgagtaaaa aaataaaata gctgagcatt ggaggataga ttgagtgata ctaagtctat
      901 cctcttgttt ttctaaagaa ctaagctaga attttttctt ttgtacgtta ttatatgtta
      961 tttgtcatta ccaaaaagca ccttttttct tctagtatct atgacataag aaataccacc
     1021 catttccttt acttgaacaa atcctactga ttcataaaat ttttttgttt tctcagtatc
     1081 atctgtcaat aattcgattt ggactggtcc ataactagtg atcttctcca aagcttcatt
     1141 caaaagcact ttgcctattc cttggcgctg atacctcgga tgaatgagga tatcttgaat
     1201 aaatgcgatc gtcgcaaaat cagtgatcca acgaacgatt ccgataatct tcccttcccc
     1261 attacgcttt atcagagatt cagattgttc aaatgctcta gctaaccttg cagtgtcttt
     1321 tgtgtaattc gtccaaccaa cgctcttata taattcagaa atctcatcga tcataaggga
     1381 atcaatattc atttttttct tcctttctta tacaattctc cctctaataa tacagttctt
     1441 tgactattta atctatcaca tataactctc ctgtaaagat tctctcgatt ccttaaaatt
     1501 cctctttcct tttagaatgc atactttttg ataaataaaa aggttgtgat aaattttctt
     1561 cacaacctct gtttctctct atcaagtgtt atcttttaat tttctaccct taccctagca
     1621 tctctattgc ccgtagtatg catactttcc atgtttgcga aaataatgtt tatctagtaa
     1681 atattgcggg atttcacata tattggggtt gatcagctcg ttttctttgg ccatcagaca
     1741 aatttcatct aagatcaaac tgttttctac tgctttcata ggtgaatccc cccatgtgaa
     1801 cggtccatgt ccatagacaa gtactccagg gacttcgttt ggatctaatt ttctttcctt
     1861 aaacgtctcg acaatcacat tccctgtatg aacttcgtaa gcttctctta cttcttcttt
     1921 cgttaattgg cgtgtacaag gaaccttacc ataaaaagca tcagcatgag tcgtgccata
     1981 agctggcaaa tcgcgtcctg cttgcgccca catgacggag tgcgtggaat gagtatgagt
     2041 aatagcagtt atatcttcaa aagtttgata caaaacaaca tgtgtgggga gatctgaaga
     2101 tggttttaag ctatcttctt ctattatatt tcccgacaaa tctgttacca ccatttgatc
     2161 tgcttgcatg cattcatatc tgacaccgct tggcttaatg acgatgactc ctagctcacg
     2221 gtccacttca ctcacattgc cccaagtcaa tttgactaat ccgtatcgcg gcaatgccag
     2281 atttgcttca aggacacgtt gcttcattgc tgcaattttt tcttcacaag acatcatagc
     2341 cagcctcctt caatttagaa cttaagtagt cttttgcctg ctggatttct cttcgaaagt
     2401 cttggctttt ttcactccac atttcaatca gaaatgttcc atcataatct agacgtttca
     2461 aatgtcttaa taatccaaga aaatcaacac aaccgctacc aaaaggaaca tcacgaaact
     2521 ggcctgaata ttcttttgtt acagggtaag tatcttttaa gtggatagca gtgatataat
     2581 cgatcccttt ctcaagttct cgagccggat tattttccgg ccatgcggac aaattgccta
     2641 ggtctggata tacttgtaga aaaggtgaat gaatctgttc tttgatttct aaaaattttg
     2701 aaatggagtt cataaatgga tcatccatga tttccacgga aagaatgatg ccatatttag
     2761 aagcttcctt tacgctttct tttattcctt cgataaagtc ttctctggaa ttcattgatt
     2821 tctcttcata ataaacatcg tatccagcta tttggattac tttgacgctt agattatgcg
     2881 ccaaatggat tgcttttttc attagttgtt tcgctgcttt tttcttttct ggatcagcag
     2941 aaccaaacgg aaatcgccga tgcgcactta aacaaatcga atggatacgt acaccggagc
     3001 tgaacatctc ttctctcagc tcagctatct tttcttcaga ccaatctaat cgtgctaacc
     3061 gctcatccgt ctcatcaatc gacatctcga taaaatcgaa gtccatttct tttacaaggg
     3121 aaaaacgttc tttccatgaa atatctttgg gtaacgcttt ttcataaatc ccaatttgag
     3181 ccatttcctt acccccagat acgaatgatc tcatctttga agttttgtgc ggccttcatc
     3241 ggatcatttg ctgctgtaat accacgacca gtgataaagg tatacacatc gatcccttca
     3301 aataagtgta atgtgtgtgg atttaatcca ccagttacag aaacattaaa gcctagttca
     3361 atcagttttt tgatcttcga taaatctttt tcaccccaag tttcaccaga taacagcgca
     3421 tcacggcttt gatggtagat tacctgccgg ataccgatgt tatgccaatc catggcttgt
     3481 tcaaaagtcc aattgccgta gagttctact tgcaattcac ctatttcttt ttgtgctgcc
     3541 tccatagttg gaatcgttgc acaacaaatg actgtcatcc agtctgctcc tgcttttgca
     3601 acattactcg ccactgttcc tccagcgtct gcacatttcg tatcagcgac tagtttttta
     3661 tctgggaaca tactgcgaat acagcgaatc gctttttgtc cttcctgtaa gcaaagaatt
     3721 gtgcccactt caataatatc tactacatca ccaactttca taacatctcc taatgcatgt
     3781 tctaagtcat tgtgatctaa cgcaatttgc aaattcggcc tgctcatctt agttccttcc
     3841 tttacttgtt tttttggtac cactttgttt cagcaaattt ttcactgatt tctttgactg
     3901 acatcacatt tttgataccg ataactgtaa ctcctttttt ctctgcctga gtaaacatat
     3961 tcaaaaaatt gactggcgta aaaactacat cgtattggcc agctgaactt tttccctctg
     4021 aaatggcaca atgatggatt ttggtaattg gtacgtttag ttctttcatt gctttttcca
     4081 cacttctcat catcatcaag cttgttcctg atccatttgc acacgatact aaaattctca
     4141 tcttaatccc tccattatga gtgttgtcta atcagatttt cttttatact ctctgctttt
     4201 ctgcatatgt gtcatagtct tctacaatca aaaaataacc atcaggattt ttccgatatt
     4261 gtaactgcgg aatagccaat aaaccaatga caactacggc aattccagca taacctagga
     4321 ctttcatgat tactgtaaac aatggccaag ctgttgccca gtcgaacatc ccgatataac
     4381 caccgtatgt tgacatccca atccatgtag cgaataatgc tgaaccgcct acttgtatga
     4441 ttcctgaaag aaacgggaac aggcaagcgg ctttgaatcc acctcggtta ttggcataaa
     4501 ctgctataac ggcattatca aagaacaacg gaatgaaacc ggctatgacg atcgttggtg
     4561 atttcaataa aatcagtaaa ccaatcatca aaaactgtcc caaagcacca aacaagaacc
     4621 caatcgttac cgcatttggc gaaccaaagc caaaagtagc agctacgtca attcctggaa
     4681 cagctcctgg taaaatcgtg tttgagattc cttggaaaga ttgagttagt tcatcgacga
     4741 atgtccgaac acctaattgc aagatcgcca gatataccgc aaaatttaaa gaagtcgtca
     4801 taatgtagaa taagaaactc tgcccttctt gcatgaattg cgcttgaatc aaataatctt
     4861 tgcctagtac aatcagaatg atcccaaaga aaaatagcat gagtaacgaa gtagcaacca
     4921 tattctcatt gaaaatcgac aagaatcctg gtaatttgat atcttcaatt tttttgtctt
     4981 gcttgattga tctatttttc ttttcatccc gctttttcat ccactcagct aattttgcaa
     5041 atataaagat tccgaacatt tgctgatgtg ccactgcaaa tccggcacct tctgtcaatt
     5101 cctgcgtgat tcctactgtt aagttagaag ctactgtcca ataacaacct aaaatcagtc
     5161 ccataaaaat caatgcttgt atcctaccta gatcaggaaa gcaaaacagt aaaatccaaa
     5221 aagcggtagc tgcttgttga acctgaacgt ttccggtagt gaaaacggaa cgcagtttcg
     5281 tatatttctg aaaacggact aataagatat ttaaaacaaa tgcaatcagt aataaaatca
     5341 tcacatctcc aaaggtacgg ccaaatgtct ccattaatcc agcatccaca gcattttgtc
     5401 caaaataagg atctgtaacc atggcatcca aattgaaccg ttcctttagt ccaaccaaga
     5461 ttggccgaaa attattgacc aaaccaccag agccaacagt caaaataaag taaccgaccg
     5521 ttgcctttaa gaatccagcg atgcattcat gaagcgggcg tttcagcaaa atatagccta
     5581 ataacacgat aaaaccaatc aggtatgctg gctgcgtcaa gatatttgtt gcaaaatacg
     5641 tccaaatgct taacaaaaat tcccccatct tttttctcct ctatcaatta attatattct
     5701 gccatcacaa cttggtaatc atcgatggag ttcgtagaga tgagacgctc aaccaatcca
     5761 tctgtcatta ataattcgga taatttttgg atattttcta gatgttcttc tggatctttt
     5821 gctgcgagtg taaaaaacaa actagccgtt ttctcctcat tctccccttc actttcaaaa
     5881 cggattggct gtttcaactt ggtaaatgaa atagctgttc caaaaacacc atcacttttt
     5941 tctgaagaat gcggcattgc tacttgaggt gcgatcacaa tataaggacc gtatttttct
     6001 acacaatgga tgatctcctc cacatacgtt tcatcaataa tttccttatc gatcaataca
     6061 agacagcttt cggcgatagc ttcacgccaa tccgtgattt ctttatctgg gtaatgaatc
     6121 aattgatggt caaaaaagta tttcagcaag aaactcccct tctttcttat aaaaacgacg
     6181 gaaacggtgt atcattttca atcgagaaga catcttcaaa tcctcgataa aaattaaact
     6241 caagatcatc tttgtttgtc ggatagatga atttcccgcc tacttgccag atataaggtt
     6301 taaatccata cttcaggcga tcctttttga atttccaaat ttcagtgatc tcttttggat
     6361 cagccataaa attagaccaa atatcgtagt ggacaggaat cactactttt gcattcaatg
     6421 actccgccat gcgcagcata tcaacagaag taactttgtc tgttatccct cgtgggtttt
     6481 caccataggc ccctaaacag acatcgattt tgtgttcatt accatgtttg gcaaataaat
     6541 tggagtaatg tgaatctcct gcatgataaa gattccctcc tgatgtttcg aataaatagt
     6601 ttactgcaat ttgatccatc tcctgaggca tttttccttt taatacctct tctggatctt
     6661 cacaagtgac caacgctgtc cgatcaaacg cttctaaagc tactacattg atgtctttga
     6721 ttttgactgc atcacctggc tttactgtaa tcgttttttc ttcaggaatt ccccattttt
     6781 gccacgtgcg aaccacttct tttggcccaa tgaatgttgc ttcaggacaa ttttgatgaa
     6841 ctgctgcggc tgtatggata tctagatgat cggaatggat atgtgtaacg attaaagcgt
     6901 ctacattttt tatcgcaaaa ggatcgatta cgaaaggctg agtacgtaaa ttaggctgca
     6961 tattttgaac gccactcata cgcatcatct gatggccttt tttcatcatg ccattcccat
     7021 gactacgttt tcccgtccca caccatagat cacataaaat attcgttcct tcatgacttt
     7081 tcagccaaat tcccgtacag cccagccacc acatagcaaa agttccagga tccactactt
     7141 ctgtttcgat ttcttcattc aagtatgtgc cccactcagg aaatgtttct actaaccact
     7201 tttcttttgt tacttcattg acagttttac tcatttgctt gctcccccca tatcgttgtt
     7261 ccaacttaca agtaaatatt aatgtgtatc agcatccatg aaaacccttt tatatgaccg
     7321 tttgtttttt atatgatttt ctttcagtaa tcaatgattg tttcagtata ataaagacag
     7381 atatgaatgt ttaaatgaac aactagtcaa ataaatccat ggatcaagat gtccctcagg
     7441 aggcaaaagt catgaaaaac tcacttgtca atattgaaaa acgtcaaaaa gaaattttgg
     7501 aactgcttca gcagactgaa caactatcaa ctttcgaatt agctgaatcc cttgatgtat
     7561 ctattagtac gatacgaaga gatttaaatt tattagaaga aaaaaacgag atcatacgca
     7621 attacggtta ttgttcttat aattacaaaa accagactga ttttgatcaa tctggtcctg
     7681 aaaggattaa acaagcgatt gccagagaag ctagtaaata cattaacgat tatgatacgc
     7741 tgtttatcaa ttctagctcg actgcgttga aaacactgga ttttcttcaa tctaaccatt
     7801 tgacagtcat tacaaacaac gtgaaaatca attactctcc gcacaaagat aattgtagtt
     7861 atattttaac tggtggagag atgcgctttc caaaagaagc actagtgggc gacatcgcat
     7921 taaatacgat ttcttcagtc aatgcagaca tttgtatcat tggctgtagc ggagtcgatg
     7981 tagtacatgg ggtcacaaca aagatattaa atgaatcaac gatcaacgaa atgatggtca
     8041 acaagacaat caaatgtaag attttagtcg ctgatcatcg aaaaatcgga ctgacatcaa
     8101 agttcaagat tgctgatatt tcagtctttg attatttgat cactgataaa ttttgttctg
     8161 aaaaagtatt agaggagatc agaaatacgg ggataaaaac cattcaagta gattcatttt
     8221 aagaaccagg acgtaaatat gtaaatagga ttatcaaaaa aatgaataac aaatttatcg
     8281 ataaatgaaa aacacgctac gctcaaattg ataaaggaat cccaagatgt atccatccaa
     8341 tcgataccat cataatctat tgcttctttt ataaccgtta tatgttgcct aaataggaat
     8401 caaacaatat cgaaaaaagg aggtatgaca aaagtcacac ctcctttttt cgaataaacg
     8461 gtattcaaat cagaacactg atttcacaac cactttattt caggtcgttt tcaatcacct
     8521 ttaccgcgta atcatttccg ttgaaacgcg ctattttttt tgcgtccata tatctttcta
     8581 aataatcatc ttctcctaat tgcttatggc aagaagcaat ttcataaagc aagtattcca
     8641 atgcataagt cgatttatgt ttcttattat actcaatacc cttctcacat aattggattg
     8701 ctttatcata atcctcgatt ttacggtaat aggtggcgat atcataatat acaatgcaga
     8761 ccatcagatt atcaaaatca gtctcactca atattttaag tgtacgctcc acataaactt
     8821 tcgcaaaatc taattctcct tttgacaagt agatcattgc cacgtttgca gctgcttgta
     8881 agtgatggat atctgtttcg gtcgtttgtt ccaacacttg attaaaataa aataatgctg
     8941 catctggttt tttcgcataa agatgatcaa gcagtccttg ataaaataaa atttgattta
     9001 aaagatcaga attacttact ttttcctgat cgatcgtttg taaaatttta taagattctt
     9061 ggtattttga tacagaattc aatgattgaa tctcatttat ttttttcctg attttgtctt
     9121 cttctgtttc tactaatata tcggagatct cgacattcag ctttttgcaa agcgcactta
     9181 agatattgat gcctggcgac atattcttgt tttcgatatt gctgacagtt gcttgttcac
     9241 aaatgccttc tgctaattgt acttgtgtta accccttttt ctttcggtat tcttttagta
     9301 atgctccatc tactttaatc aaaacaattt ccttcccttt aaaaaattat ttctccttta
     9361 tatcatacac aaaaaaaagt tctaaacaac caaaaatgtt tagaacagaa taaaaatttt
     9421 aattttaaaa taatattcaa attaaacaac tggcgtatct gctccttgat ttctttttgt
     9481 gttatctgaa taattatcgt ttacagttat aacacatagt gaaaattgtc atggcgagaa
     9541 agtctaagaa tatttttaaa cataactatt tctttcttac gtgtgtataa tgacgacagc
     9601 acacctatca attaatataa ttattttatt tttttatgtt ctcatgttta tttttaatga
     9661 cacaacttct cgtatttatc tggcacaaca tataaatcga tcaaaaaatt gttgcgtgtt
     9721 tttttgaaaa ttggagtttc aacttgatta caaaatttaa atttagaagt tgtgaaaaca
     9781 gcgagagaca gtttaatatt caaaatatac ataggctact cgaacgctgt gttttttagt
     9841 atactagtaa gaaacgaagg gatagactca ttccaataag gaggatttca tgaagacaat
     9901 tctttttgct cctgcagtat tcaatttagc tgagacaaca cgaatgattg aggttgccaa
     9961 agctatgaaa gaccgttatt actgtgtatt ctttggcttt tctaaaactt ttgctccttt
    10021 gatcacagaa agtggctttg agtatcattt actagcgccg atccttacga aaaaacaaga
    10081 agaacaaatc ataaaatttg accagttgcg tacacttaaa aaccctttta ccaaagaaat
    10141 ggttgcctct cgtatcacta gtgaacagag tctgattgct aagtttgagc ctcgactgat
    10201 tgtgacaggt tcgaatgtga caattttttt atcggcccgc aataaaaaga ttcctttagt
    10261 atatgttaaa ccctatgctt tgagtgaacc tttttttcaa tcagagcatg cgctgatgcc
    10321 ccacgagtta cgtttcttcg gccctttaag caatcccctt tggcactgga caaaaaagcg
    10381 tatagtaaat acccgatgga tccccaatgc cttcaaacac gctgcacaaa aagaaatgct
    10441 tcctttacca aaaacaagtt tacaaatgat ggatgctgat ctaaatctca tcacaacgcc
    10501 taaactattt ttggaagaag aacagctgcc ggaaaattat cgaatcgttg gtccaatttt
    10561 tgctaagctc agtacttcct tgcctgaaga agtgctaact tttatacagc gaaagcaaca
    10621 agaaagaaaa cgtattatct atttcgcaat gggtagttca ggcaatccgc gtatgttacg
    10681 caaaattttg ttcttcttac aaaaacaacc ggatctagcg gttattgcac ctattgacta
    10741 tctgcttgat gacattcaat ctgactcaga agatctttta ataacagaat atctgtctag
    10801 tcatcttttg catgaattca ttgatttttc ttttatccac ggcggggaag ggaccgtcca
    10861 aactgcctgt gaatccggca aaccgtttat cggtgtgggg atgcaatttg agcaaagctg
    10921 taatattgac tattgtgtaa aatacggaaa cgcaatcgcc ctcacaaaac cagtgactga
    10981 aagaaaactg cagattgctc tgcgcgaagt ttcttcaccc aagattcgtc agcaggcacg
    11041 cacgctgcaa gccgtttttc caataaatgg tccacaacaa gcaattcaag aaatcgaaac
    11101 cttcctgagc aaccacccaa tatagtttca agtagaaaaa gcttttttaa tagagactca
    11161 tcagtaattt cgctattcga gacccagaca gtgaaaatac aaaaaaagta aatagtggat
    11221 tgaaaagtta gagttactta aattacggtg tctaaaaaac acaaaatcat ccttaaaata
    11281 aaagatctga cgctatccaa atgagcatag gtagcgtctg ccatttattc ttagtcaaat
    11341 tctttgacac ctttctctgg ttggatcatc tcaacgtttc ttcaaaagaa agaccataat
    11401 aattaggcaa atacattttc ttaaaatgtg tcatatagat ctagaagcat cgctttgtag
    11461 agatttgtgc cgctagtccc ctcaacaatt actaaatcaa atgtaggatt taccacttta
    11521 tctttgataa tcaacctccc tcttctgttg agacaatgat ctcgcaatcc gatgcatact
    11581 cctatataaa ctgccagcaa tccccacata ttgagtagat catgtcagaa tgttctatgt
    11641 cgtcagcaac gatacactaa aagtcacgtt caccatgact gattgctatt cccagcacaa
    11701 ttatttctat acacgtttca tgatttgaat aaacactcac atcatacata ttaccattca
    11761 tacaccatac tgcagcccct actatatggt tgtaagctcc caaatctata tttgacttga
    11821 tcactttggt agtagatcta ctaattctaa atccattgct gataaaattt ccattttttc
    11881 ctccttcttt ctgccaaaac agttaaaaca ataataatct ttatgtaaaa aagggctgtg
    11941 acaaaagcct tgtcacagtc ccatattccg aataaacggt ggtcaaaagc ttgcatctgc
    12001 ggtaataagt tcaaccaaac gaaaaataag ccaacatttc acaaaaattt aagaagcaat
    12061 tttcgtaaat ttcttcttat ttcttgaagc taaccacttc tgttacaacc acttttagaa
    12121 catttaaagc ttcgatgtga caaaccatct atgctgctcg atcataaaat tgatagcaaa
    12181 aattccacaa agtacatatg tcccccatga aaacaaaaca ttcatatgcg gtacaatgat
    12241 ctttggaaga atcacgatca aaaatgatgc cagtgcccaa atccatcgat tgatttgaat
    12301 atgatatgta gctagcatat tcaatataag gtgactagcc gttaataaaa agataaaaac
    12361 aaaaaataaa atcagccaat acattgtttc ccaccttcta aaaaccaaac ttagacagtc
    12421 tggacttcgt tttctccacg actttgttca tctgacagca tctttttatc ataagctttt
    12481 acaaatggat aatagataat aaatgctatc aatgtgttga tcatgatcaa gataacaaca
    12541 cgccagtcat ttcctgctga aagataaccg ccgattggag ctggtaatgt ccatgttggc
    12601 aaaacagtaa atctattgac catttctaat ttgactgcaa aataactgat aatagtcaaa
    12661 actaaaggaa ctaagttgaa cgggatgaac atgtaaggat tcaacatcat aggagctcca
    12721 aaaattatgg gctcattgat attgaagata ccaggaataa tagaaaaacg accaacctct
    12781 ttcaagaatt ttgatttaca gaagaatata aacaaaacag ataccacgat cgttgctcct
    12841 gcaccaccaa ttgttaccgt ccattgataa aactgttcag gagcgatata gggtaattgg
    12901 gtggcagctg taccatctgc caatgccttt gcattctcat ccaacatgat cagccacatt
    12961 ggacgaacaa cagcccctac aatactcatg ccatggatac caaatgacca caacaaatgg
    13021 atcaaaaaca ttggtagtaa aacaccaagc aaattattcc ctaaaatatt ggttaaaggc
    13081 tcgaataacg ataataaggc agcgtttaca tcaaagcccg cgacgcttcg aatcagccaa
    13141 gtaattgtta aaatgactgc acctggaatc aatgcctcaa aactacgggc aacagcaggt
    13201 ggcacttgtt ctggcatttt gatagtaata tttcgatttt taaagaaacg atatacttcc
    13261 actgcaaaaa tcatagataa gatccccgca aacatcccag atgctcccaa agaactcata
    13321 ggtaagaccc agccaagagg tgcctcttca ggagttaaaa cgtttacagg aacttgtaac
    13381 ataaagaacg ttcctaaaga taatatacca cctgtcacac tatctaatcc atatgattta
    13441 gctagatccg aacccataac aaaagttgca taaatagcca tcaatcccat cgtcatccta
    13501 aagggtaaaa caatcgttgc cgaataaggg gcaataaatt cattccatcc aggaaacggt
    13561 aaattgataa agaccataaa aaatgtccca atcaaaatca atgacaatgt tgaaacaacc
    13621 ccattacgaa ttgctaataa atgacgctgt gtaccaattt ttgccattgg tgggacaagt
    13681 ttctcttcga cccatttcat cagcttttcc actaaaacca ctccttttca ataaaatatg
    13741 aaccattcat tgcatgacaa cgcaaagaat gttcataact aattatataa ttaagaaatt
    13801 ttatttcaat atattctctt aaataatcaa atttattttt aagaatcctg aaaataagtt
    13861 ttctaaatca taggagcaac aaaaaaattc gataattaac aaaaatcaaa atgattaaat
    13921 ctagtgattg aaacatatca tttcaaaaaa attttgtaaa ttcacccata aatatcaccc
    13981 tatttaactt ttttcatcgt acactaaaat tcaaaacaac tatctttaat ctttacttct
    14041 tggttttgtt ttaataaatt tccttcaaaa atgcctggag aaactacaat gatcaccgta
    14101 tctttttgtg cttgctccaa cttcttcaaa tctataatag ctaacagtgt cccttctttt
    14161 acctcctggt tactttcaac ccgtatctga aacggacttc cttcgagttc aactgtatct
    14221 aaacccatat ggatcagtat ttttctgcca gattcacttt cgatcgttat ggcatgtcgg
    14281 gtaggaaata tattatcgat tattccttta actggcgcat ggatatatcc atcatgattc
    14341 gttatagcgt atccaactcc tatcattttt gaagaaaaga cctcgtcttt tactgctgtt
    14401 aatggaataa tttgaccact agctacagct tttagaatgt ttcttgtttt aaagaaatgc
    14461 actttttatc acctctactt taatgctggc caatattttt tgtttacttt gatcatttca
    14521 tctagtattt tttttgcgac tctggcattt ggtacggttt gattcaatgt aaatgcttgt
    14581 aaagcttttt gataagaacc ttcaaagtaa gcatctacta gtaacttctc tactgcaact
    14641 tgtgcctcca tcaatccttt atgaaaatca ctgattggtt ttcttagtgt aattggctcc
    14701 acaccagttg caccaacata tgccggtatt tcaacaactg catctgaccg tagattcgga
    14761 atcgcccctt gattaggaac gattagcata aagcgacgac gatcatcatt caacaaggaa
    14821 atagctatat cgacgatata ttgtccatgc tccccaaaat taaagttcaa tacttcttcc
    14881 acaataccgc ggcgtatttt atcagccatt tctttggtat ttttctctcg tccatccatg
    14941 accatattag cacgtgtata ctctttgtca gtatgctcaa cgaccatatc aggatataag
    15001 taatactcta agtaattatt cgggatatta cttggaaaat ttttcgtgat gactgacatc
    15061 atttgaaagg ttttttgcca agttttatct cctatattaa aatcggcgac ttgcatttct
    15121 tttgttatta gtttttcaat aatctctggc ataatttctc gttgcaattc tttatcataa
    15181 atcgatgtat accagccaaa gtggtttaaa ccataatacg taacgatcca attcttacga
    15241 tcataaccgt aattcactgc aatcgtttct tcaattgaaa tagtcatatc acaggcatta
    15301 atcattttta cattcggata tttacgacga acagcttcag aaacgatcga ctctggattt
    15361 gtataattca aaatccatgc tttgggggca tagtcttgta tatgatcgac aatttccaat
    15421 aaacctttga ttgagcgcat cccataagca aatcctccga gtccacacgt ttcttgtcct
    15481 actaatccat gtttaagtgg aatcttttcg tccttttctc gcatctccaa tcctccaaca
    15541 cgaatttgtg agaaaacaaa atcgcatcct gtaaaagcaa gctttggatc atccgttgca
    15601 cgaatgcgaa tatttgaaaa accttttctt ttcagcataa atttaacaat caagtacata
    15661 tccatatttc tttcttcatc aatatcatat aaacgtattt cagataatgg caaccgtttt
    15721 tcattgttca aaatggcttg aacgattcct ggtgtatacg tactcccgcc tccagctata
    15781 gtgataatct gttttttcat catcatcttt tcctttctat tcttctaata atttattgaa
    15841 ttctgccata acataagtca cttctaagcc gatgataatt tgataatttt tttcagttgg
    15901 tttaatgatc ccgcttatcc ctaattgctt gattcgctgt tcctcgacca tggtgggatc
    15961 cactacctct acccgtaggc gtgtattaca actatagaca tccaggatat tggcttttcc
    16021 tccaaacgct tgtagcattt ttgttgccat atattcataa tttttacttg ctaaccctaa
    16081 ctctgtttct tctaaggtta cttcattgcc aaactctgcc ttagcttcac gtcctaatgt
    16141 ctgaatgttt ctcttattga tcaaataata aaaagtaacg taatacaagg caaaaaagaa
    16201 aattccgatt ggaagaatca aaatagcatt ggtagcgatt cgaaaattga tgagataatc
    16261 aacaatacat gctccgaatg taaaaccaat tcgtatattg aataagtaac agacaatccc
    16321 tgctaaaccg gcaaaaagtg catggatcac gtacaacata ggagaaagaa acatgaaact
    16381 aaattcaaca ggctcagtga tatttgcaat aaatgaagtt cctgcaccgc ttcccatgat
    16441 tcctttgacc atctctttat tttctttata agctgcttta tagatagcta gacatatagc
    16501 tggtatacca aacatcatta ctacaaaaaa aactgataaa aacagtccag cctgaggatc
    16561 tccgttgata aagcgtgtca tctctcctct gaaaacttcc ttgctaggag atgtatagct
    16621 tcctaaatcg taataaatat acgtattcaa cacatggtgc agtccaaagg ggatcagcaa
    16681 acgattcaaa aacataaata tccccacacc taatgctccc atatcaacga gcagttttgc
    16741 aaaattatca atcccaattt ggatgagcgg ccagataaag cccaaaaaaa cagctgcgat
    16801 tgtttgtaga atcatcacca tagtcaatgg gaatttttcg cccgcaaaat aagaaaaaac
    16861 tactggtaat ttttgttctt taaagcgatt atacgtccat gccgctacta aaccggaaat
    16921 aattccacca aaaatcccca tattcacttc tgggtccata atttctaagc cttttttcaa
    16981 ggtagcaatc gctaaaaaac cagtaagtgc aggtattcct ttgtccttat ttttgacaaa
    17041 accgattgca actcctatag caaataagat atccaagttt ccaaaaacgg catttccagc
    17101 tgcaccaatt actgggatgt tcagcatatc gtcagctgat aaacgcatga tcaatcccgc
    17161 taatggcatc gctgcaactg gaagtagcat cgctcttcca agcttctgca catttgattt
    17221 aacattcatt tttattcctc ctctattaaa tatcctcata tgttactaac tcaatcaaag
    17281 gatcattttt cactttttga gcaaatgctg aatgaattaa aaattcgact tggtgggtac
    17341 gaggatacac aaaacttgaa ccatttaaca attcctgatc caaatacgct gcgtgacaca
    17401 tgacttcgat cgttttatct tctctcaata tcatgagtaa acttgccaaa tattcttcca
    17461 cactcgtatt gatttgtcgt tttttttcat cagtttctcc tacgacatcg aaacaaggct
    17521 caaaataatc gacatgacga acttcatctt ttcgttcaaa gttgctccga accggcaaat
    17581 catatttttt tgctaatgta acaactacct cctgattttc atccaaagta tgagtatgat
    17641 gatgactgtc taaatgagtt ggtatgatcc ccgcatgata aattttttga atctgagcat
    17701 tccattcctg atacagttga tctttgtcaa tgttaaatgg atgctgataa taagataagg
    17761 atctgaactc tccgctttcg ataagagtat cgacagtttt gaggagtggt ttgccacacg
    17821 tcaatgttaa atggactcct acacctagcc taggcatttc ctttcttagt ttcacagcat
    17881 gctcgaaacc cggcatattt gccatcaatg tagttgatgt caaaattcct cgctgataag
    17941 catccataat cccataattc acaccatggc tatagccaaa atcatccgaa ttaaaaatca
    18001 cttttcccat ttcattctcc ttcttttttc tactaaaata ggcgcaacca aataatcata
    18061 gtccatttac tatgtgtcat ttggttgcgc ctatagatag ttacgccctt attttaatag
    18121 attcttgcta gatgaattgc caaatagatg atttcatttt cttgaatggc aagttgaaat
    18181 tgctgttcga tgatatcttt aatctttagt gctgtacaat acgctttgct atgtttaaat
    18241 gtttcagtaa aaaaaccaat attttctact ggcgtttctt cctgataaat caagcgttta
    18301 accaggaatt tgatatgaac cattaagcga tgataagtca gtgattcttt atcataaacg
    18361 tttgaatagg atttttctag atgggacaaa atcgtggtcg tcatttctac tatttttaag
    18421 ctctttaaac tattaggctc tcctagctca gcattaatga tatgcatagt gatgaatcct
    18481 acttcatttg accctaactc ggtttgatat cgatgattga ttcgctcgac cacttctact
    18541 gctatatcat attcatttgg ataaaggaat ctcatttctg gcaacataat tgctgtgaca
    18601 tctctatctt ctctttttcg ttgaatagca aaataaatat gatctgtcaa tgtaatatag
    18661 atatttgaat ctaatggaga ggagatccta gctgttatca gttcaatcac ttcttccgca
    18721 aaatccataa attcgattgg aatctgcgag actatctcac tgaatcgttt gatcttttct
    18781 ttagaatcta atacaaataa tttgtcagca tcctcaacat caaatggctt actcacttta
    18841 gcagaaaagc caatcccttt ccccatcgca attttttcta ttccatcgtc atcgatcaat
    18901 acagcattat gattcagctt tttcatcaca tacaccgtta attcacctcc tctatatgat
    18961 aaaacaaaac aacttgcaga ctccttcata catatttttg tctccaaaca atttttaaag
    19021 acaaaaaacg acctaagaaa gaaccctttc ttaggtcgtg cctaattgaa tagtagcacc
    19081 ctacataatt tacaaaaata gtttaacaca taataaaagc gttatcaata tcttttttac
    19141 tcgttttatg ataatacttg tatcgaattg atttggaggt aattacataa aaacaaaaaa
    19201 aatatagcaa ctctctataa gaaagttgct atattgataa ttataccggc ggccggggtc
    19261 gaaccggcac gtcctcaacg gacactggat tttgagtcca gcgcgtctgc caattccgcc
    19321 acgccggcaa atttaaaaat aataactggg gtagctggat tcgaaccaac gcatgaggga
    19381 gtcaaagtcc cttgccttac cgcttggcta taccccaaga ataaagggcg aatgatggga
    19441 atcgaaccca cgaatgccgg agccacaatc cggtgcgtta accacttcgc cacattcgcc
    19501 atcataaaag atgttattta atttctcaat aaaaaactaa gatgaagaca tcttagtcaa
    19561 taccggcggc cggggtcgaa ccggcacgcc ctcaacgggc actggatttt gagtccagcg
    19621 cgtctgccaa ttccgccacg ccggcaagtt gatttattag tactaaggcg gtaaccggat
    19681 ttgaaccggt gatgaaggtt ttgcagacct ctgccttacc acttggctat accgccatta
    19741 tactattatc aatctataaa ataaagaata aattatagaa actggggtag ctggattcga
    19801 accaacgcat gagggagtca aagtcccttg ccttaccgct tggctatacc ccaagaataa
    19861 aaagggcgaa tgatgggaat cgaacccacg aatgccggag ccacaatccg gtgcgttaac
    19921 cacttcgcca cattcgccat ggcaggggca gcaggaattg aacccacact aacggttttg
    19981 gagaccgctg ttctaccttt aaactatgcc cctataaaat ggaggagagt ggattcgaac
    20041 cactgaaccc taaggaacgg atttacagtc cgtcgcgttt agccacttcg ctactcctcc
    20101 atggtggcgc gggacagaat cgaactgccg acacacggag cttcaatccg ttgctctacc
    20161 aactgagcta ccgcgccata ttttaaaatt attaattgaa taacaagaac gacggttccg
    20221 acgggaatcg aacccgcgat ctcctgcgtg acaggcaggc atgttaaccc ctacaccacg
    20281 gaaccagttc attatggagg ttaacgggat cgaaccgctg accctctgct tgtaaggcag
    20341 atgctctccc agctgagcta aacctccaac attcaattaa aatgacccgt acgggattcg
    20401 aacccgtgtt accgccgtga aagggcggtg tcttaaccgc ttgaccaacg ggccaggtaa
    20461 atttgctacg gagagtaagg gattcgaacc cttgagacag tttggcacca tctacatgat
    20521 ttccaatcat gctccttcgg cctctcggac aactctccag agtgaaagaa gctcaatact
    20581 ctttctcaaa atgag
//
LOCUS       DACEEZ010000049        19473 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20313_2017
            SAMN12639966-rid9879223.denovo.049, whole genome shotgun sequence.
ACCESSION   DACEEZ010000049 DACEEZ010000000
VERSION     DACEEZ010000049.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639966
            Sequence Read Archive: SRR10023158
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 19473)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19473)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741683.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741683.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:44:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,016
            CDSs (total)                      :: 2,962
            Genes (coding)                    :: 2,889
            CDSs (with protein)               :: 2,889
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 36 of 73
            Pseudo Genes (incomplete)         :: 29 of 73
            Pseudo Genes (internal stop)      :: 18 of 73
            Pseudo Genes (multiple problems)  :: 8 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19473
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639966-rid9879223.denovo.049"
                     /isolate="E20313_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(21..995)
                     /locus_tag="IWO89_001780"
     CDS             complement(21..995)
                     /locus_tag="IWO89_001780"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HAQ0456513.1"
                     /translation="MSNKYQDDTLKIFSLNGNRPLAEKIAKVFGTELGKSVVKQFSDG
                     EISINIEESIRGDHVYIVQSTNAPVNDYYMELLIMIDAMKRASAKTINVVLPYYGYAR
                     QDRTAKPHEPITAKLIANLIEEAGATRVLTLDLHTVQVQGFFDIPVDNLFTMPLFAHY
                     YRQLGLTGDDIVVVSPKNSGVQRARSLSEYLNSTLAIVDHADDASTEGGYVIGDVQGK
                     TCIMVDDILNTGATLARAANVLKENGAKEVYACASHGLLSDHAKSILDNAPIKDICIT
                     DSVFTEENRHPENLTIVTCSELMGEAVKRIHENTPMSPLFRLEEKEFE"
     gene            complement(1075..1950)
                     /locus_tag="IWO89_001781"
     CDS             complement(1075..1950)
                     /locus_tag="IWO89_001781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoamide dehydrogenase"
                     /protein_id="HAQ0456514.1"
                     /translation="MKKRRRSQLNQVVDKPLYFKVDKKIKKLASTQQLQSRKSKLLFL
                     ALVFEDQSYVIIDQSGHPVEYSPAKYTYQEGLSCKKWKLINEEPIELSRWINRKEDIP
                     VLIEEKRSGKELANCWVGLPEERFLRYKKWATPSGYLCGTYAAAVLLAYYQDYRKGWM
                     LPNEIRKKNTADSLVLTKALRSQIQPLGLPTIPFQVSTGISSFLKKNGNHERARATLL
                     GSWQRATKRIREGKPVMIGILKVLGSTYGNHWVTAYAYFETETGERYYKVHDNWGDYH
                     KVIPASWSNGTVSLP"
     gene            2080..3987
                     /locus_tag="IWO89_001782"
     CDS             2080..3987
                     /locus_tag="IWO89_001782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M13 family peptidase"
                     /protein_id="HAQ0456515.1"
                     /translation="MNKERLKQDFYEAVNEEWQKTAKIPADKPATGGFQDLVDGIDQL
                     LMGETDDLLAHPEKVSSEKMKHFLAYYQLANDYDRRNELHAQPLQPIIEKVESLQSFD
                     ELNKQFPEWLLDTLPLPFNLDVDADMKNAQTNAFFAYPPSLFLPDKTYYASDHPNGAQ
                     LIGVFFDMAVQLLELAGKEKSQAEAITEQAVQFDKLIAPHVKSAEENADYSKMYNPQS
                     FDTFIKYTDKLDLKKLVTGLIGTTPDKVIVTDPVYFDHLNDILTEAHFPLFKSWMIIK
                     TVRSLSGYLSEEFRQVSGIYSRVLSGTDEAMPQKKAAYYLASGQFDQVVGDYYGKKYF
                     GEAAKKDVEQMVKKMIAVYKKRLEENDWLGDATKEKAVIKLNNLGIQVGYPDKIPALF
                     DQFKTVPADQGGTLLSNTLVFSKLTLKDRFSKWNKPVDRSEWEMSADTVNAYYHPFRN
                     VIVFPAAILQAPFYSLEQSSSANYGGIGAVIAHEISHAFDNNGALFDEYGNLNNWWTE
                     EDLTQFQNKAQAMIEEFDGLPFADGKVNGKLTVSENIADAGGLSCALEAAKTESDLSI
                     EEFFVNWATIWRTKAKKEYQQLLLQIDVHAPAKLRANVQPKNLDEFYETFGITEQDAM
                     YLAPEKRVRIW"
     gene            complement(4528..7806)
                     /locus_tag="IWO89_001783"
     CDS             complement(4528..7806)
                     /locus_tag="IWO89_001783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1565 domain-containing protein"
                     /protein_id="HAQ0456516.1"
                     /translation="MLKKRIKYSALMMNLLMLSTPVLISVVEVAANEQQSVNEENAHV
                     NIEGMSTMEEVPRTSEDMDTEEGNDPMMKESENEKTKKQSEEANEEWKGVVFGESTSA
                     SRYAIEPLEDGVRLSSTNNGGKFQSSGSDGMTYYYQAIPKDINFRMRATIEIESWTYT
                     NAQEGFALMVRDAVPKNHLFGQAFYSNSFAILGSRIEYVWDSDRQEVVNTSGSKYTMR
                     LGLGTRAITGISSEDPQAAPAPGSVSVETTPLETSAGFLEKETGSYNIFGNGHGNLFP
                     ATNSITKLDVEMKKTNTGLESYYYDPETGEEMASDIMYDWEKLYASDESVIYAGFAAA
                     RNMTIKVEGIEVAYSDPATDPPGQERPTRLIDPIYTVTSSNHSGNQDHTMSFRANADG
                     LLTIKNKATGEVLKNAKNLAITANREFDYQTKLMEGTNEFTFSFTPDKNYPPEEYVEM
                     TSYETKEILHTVDYRKPKYSTVYVTPEGSDAGNGSRQNPLSLTQALRYAYAGQTIVMA
                     PGTYSFERGLTIPKGVNGTRENPIQLIAEKNPENKRPVLDFKGTGNGMTLFSHYWGLR
                     GFDITNSAAMQKGLQISGNHNLIEEIHAYRNGNTGIQISGSSNDPFEAWPAHNLVKNC
                     TSFENADPGFEDADGFAAKLTIGEGNVFDGCIAYHNADDGWDLFAKNETGSIGKVIIK
                     NSVAYRNGWVPGIEGEGNGNGFKMGGSSLTGPHELINSLSFENLAKGIDSNSGTDIIV
                     NSSTSFNNGSYNVALYTSSAGNTDYHASGILSFRTENLEMREQIRPLNQNEDQIYHSL
                     NYYWNEREKQSENTLGHTISKDWIESLNTCSKEGQQPVTRYENGSIHVHGLMQIKPGN
                     RQTEKERVDGLPLSVGAIFDGETSPSSEYPEYAIVQEPNQTKPNEQNNPIIIQEANGV
                     AENAEGNIIELKPFTVFVSTFTPNQGTPVFEDMRYFPEKIQIVETQSSAQFSNHTAIF
                     SAPSKGEYTLEVTYRSEIFDGEKWSAHTESVTQQKAITVRETADVPPGNDKPERENPG
                     NESPNGKPNGKPSHPDRTAKESEKNGTQGLPKTGEFNNPLLALAGGILLIGVVVYVMK
                     QRKK"
     gene            complement(8077..8622)
                     /locus_tag="IWO89_001784"
     CDS             complement(8077..8622)
                     /locus_tag="IWO89_001784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HAQ0456517.1"
                     /translation="MNALISIDYTYDFVAEDGKLTTGTAGQVIEKKLTAHTKKFIDQK
                     DFVVFAIDAHDPKDSFHPENRLFPPHNVIGTGGRELYGSLKSLYQDYEQEENVYWIDK
                     RHYSAFSGTDLDIRLRERQITDIYLTGVCTDICVLHTAVDAYNLGYKLHIFKDAVASF
                     DPVGHEWALRHFESTLGAEIL"
     gene            complement(8634..9326)
                     /locus_tag="IWO89_001785"
     CDS             complement(8634..9326)
                     /locus_tag="IWO89_001785"
                     /EC_number="3.2.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'-methylthioadenosine/adenosylhomocysteine
                     nucleosidase"
                     /protein_id="HAQ0456518.1"
                     /translation="MKIGIIGAMEEEVKILRENLSEPLSWERAGALFISGSLGNHEVI
                     VVRSGIGKVLASITTSLLIQQYGVNMVINTGSAGGIGEGLQVGDIVISDKVAYFDADA
                     TGFGYKPGQLPGMPLYYEASTYLRSEMVKAAKATNLNAKEGLIVTGDTFVDSPDKIKE
                     ILTNFPEALACEMEGAAVGQTARQFNIPFLIVRAMSDTADHSATQSFDEFIEDAGKRS
                     AEMVIEFVKHLV"
     gene            complement(9345..9629)
                     /locus_tag="IWO89_001786"
     CDS             complement(9345..9629)
                     /locus_tag="IWO89_001786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0456519.1"
                     /translation="MAKGPLITRSELRKRQQAQASESLKKQRKAETAYQQEEKKIASF
                     YRKESKKNKPITKTRISEREKTTKWNSFLMKSLIIVILMLCVVFLAIAFI"
     gene            complement(9642..10205)
                     /locus_tag="IWO89_001787"
     CDS             complement(9642..10205)
                     /locus_tag="IWO89_001787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HAQ0456520.1"
                     /translation="MLNNEQFEEKTISRKEIYSGKIIDVAVDEVRLPDGGTSKRELVF
                     HPGGVGIIAFDEQDRLLLVKQFRKPLEKVILEIPAGKIDPGEGQNPEMTAARELEEET
                     GYRAKSLSHLTSMYLSPGFANEVLHIYHAQGVEKVENPLAQDEDEVLELYHLTLEEAQ
                     QAMKDQLICDAKTIYAIQYWELLTKGK"
     gene            10433..11095
                     /locus_tag="IWO89_001788"
     CDS             10433..11095
                     /locus_tag="IWO89_001788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-bromo-4-chloroindolyl phosphate hydrolysis
                     protein"
                     /protein_id="HAQ0456521.1"
                     /translation="MIKRNWPWLLALVLLFLFVRNNSFSFVLGLLLIGVVLVLWGLFG
                     SRRKKGKQEEIPALTDELETHYEKSGMTANEISFFRQTMNQTKNEINQLQMNMQQTAK
                     LKAIDLRHDPVKAAKALFKELVKEPNRLHEASQFLYTHLPNLVDLTNKYNEINDHEIK
                     NKQTYEKLEESAQIIDQLSALIAQDYQKFVAEDLDDIDVEISVAKQSLKRDNKYEPEQ
                     KD"
     gene            11219..12415
                     /locus_tag="IWO89_001789"
     CDS             11219..12415
                     /locus_tag="IWO89_001789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxic anion resistance protein"
                     /protein_id="HAQ0456522.1"
                     /translation="MDHKPTETKDVTPVNDTLEDLLNNPFSTPTDTLTTSQQAEIDQL
                     QEQQTAARLIDKLPAERQEQAKQLAAKIDANDAQSVISYGSAAQAKLSEFSQSMLNHV
                     QAQDIGPVGDSLTELMYRLQEANPDELRAGEGNIFQRVFGKVKQSIYEVTAKYQKIGA
                     QIDKISVKLDKEKDGLLKDNLMLEQLYQKNKDYFDALNIYIAAGELKMEELQTTIIPE
                     AMKRAEETGDQMDVQIANDYTQFLDRLDKRTHDLRLARQITIQQAPQIRLIQNTNQAL
                     AEKIQASIATAIPLWKNQVVIALTLLRQKDAVTAQRQVSETTNDLLKKNSEMLKISAI
                     ETAKENERGIVDIETLQTTQNDLIETIQETLRIQKEGKEKRRHAEVELGHMEEDLKQK
                     LLDLTQ"
     gene            complement(12516..13892)
                     /locus_tag="IWO89_001790"
     CDS             complement(12516..13892)
                     /locus_tag="IWO89_001790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAQ0456523.1"
                     /translation="MDLFRKKEINLNHTSEMKKELKTSDLIMLGIGAIIGTGIFVVTG
                     VAANQNAGPALSLSFVLAAIVVILSGLSFAEFASRVPVIGGPYAYLYVVFGEFAAWLT
                     GWLLIGEFLLAVSSVASGWSGYMQGFLKSLGAELPQALTGGYNPENGTYIDLIAVLVV
                     IFVTYIVSLEAKKALRLNNAMVYVKFGIIALFIIVGIFFVKPDNWQPFMPFGFSGVLD
                     GAALVFFAFLGFDAVAMAAEEVKNPQKDVPRGIIGSILIATVLYIIVTLILTGIVPYT
                     ELGVNDPVAFAMRYVGHGTVGAVIAVGAILTLLTVTISMMYSLARLLFAVSKDGLLPK
                     FMTEIDQKHRTPKKATYAAGVAAVFFAGFFPLNVLAELTNIMALAYLMLVSLGLLKLR
                     KMFGKPKAGEFKVPFVPLLPIVSILTCIVLMLRLQTVTWIVFGIVMVIGLIIYFGYGY
                     GHSKMNEK"
     gene            complement(13929..14795)
                     /locus_tag="IWO89_001791"
     CDS             complement(13929..14795)
                     /locus_tag="IWO89_001791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose transporter GlcU"
                     /protein_id="HAQ0456524.1"
                     /translation="MEILIALVPMLAWGSIGLVSGKIGGDANQQTLGMTIGAFLFSLV
                     VFFVVSPVITPWIFLIGFLSGLAWSVGQNGQFHGMKYMGVSVGLPLSTGFQLILNTIA
                     GAVFFHEWTQTKDYVYGIIALALLVSGAYLTARQDDEGKIDTDNKMLDFGKGFRALIF
                     STIGYGVYTIIVNWANLDAMSIILPQSIGMILGASFFAFRKVKVDQFVWKNMICGLLW
                     GLGNICMLLTVKSLGLAVGFSLSQMGIIISTLGGIFILGERKTKKELIYVIVGCLLVI
                     LGGILLGYMKTV"
     gene            complement(14884..15909)
                     /locus_tag="IWO89_001792"
     CDS             complement(14884..15909)
                     /locus_tag="IWO89_001792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HAQ0456525.1"
                     /translation="MNFFKYLKDQWTLILGWLFFIALTIFVMWLAPNIKVDLSTIAYL
                     ALIEGVFLIFYLLGGYLSKRRWWGKLADIQKTSPLQNYLSGGRTEEEKLVEEYINQLI
                     REHQEVMQSAISNQQDQKDYIDSWVHEIKVPLSASKLLVRSIEFDIDDQKYTLLENEL
                     SKIDEYVEQVLYYARLDSFSRDYLIQEYGLKGIIQPVIRSQANYFIQKNIRYEIVGED
                     HTVLTDAKWIAFIFNQLLSNAIKYTPDHGNIIVSIEKEAQGVSLSVKDSGIGIPAEDL
                     KRIFDKGFTGRNGRLSKTHSTGLGLYLAKNLAEKLGIHLTAESTEGKGTTMTLFFPIL
                     NFYNEKR"
     gene            complement(15927..16601)
                     /locus_tag="IWO89_001793"
     CDS             complement(15927..16601)
                     /locus_tag="IWO89_001793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010760322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAQ0456526.1"
                     /translation="MAKIMVVEDEEIIRQLIMEELEKWQFETFGTTDFNQVFSDFERE
                     EPQLVLLDINLPVLDGYYWCQKIREVSKVPIIFISSRNTNMDMIMAMNMGADDFVTKH
                     FQIDVLIAKINALLRRSYNYTELSSEMMSHNGITLNVDNGSMEINGEIIDLSKNEYRL
                     LFILMKHNGKILSREKLLRALWDDERFVDDNTLTVNINRLRRKIEQAGIQGYIETKVG
                     QGYIVP"
     gene            complement(16785..17678)
                     /locus_tag="IWO89_001794"
     CDS             complement(16785..17678)
                     /locus_tag="IWO89_001794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HAQ0456527.1"
                     /translation="MKKKIGNRPQTQISLLLQLFMAVMFLAGAAVFTYPFLADALSNY
                     LDQRRIENYQKQLERDKEEKHEQQLAVHEKKNQTLAHTSVIPGMGQVKDPFEQAVRNV
                     QNPGKEYYEQHMIGAIYIPKINVSLPLFDETNDLLLDRGATVLQGTSFPTGGESTHSV
                     ITAHSGIAEKKLFTDLEEMEQDDRFYLEVYGRMLAYEVVEKIVVLPTKTNTLAIREKQ
                     DLVTLITCTPYTVNTHRLLVTGRRVPFTEEVSSKIEQTKKYHLYRLLILLLGILLVLT
                     LFGYWCYRKFKRQKQRKKNNR"
     gene            complement(17752..19449)
                     /locus_tag="IWO89_001795"
     CDS             complement(17752..19449)
                     /locus_tag="IWO89_001795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaH/EbpB family LPXTG-anchored major pilin"
                     /protein_id="HAQ0456528.1"
                     /translation="MNKKMIVTGMSVLLLVTAGIGIFGNGKLVKAEEVAQKPSEVTIT
                     LHKKGFSSVPEERPNSGLVSTDFGEENIPGVDFDLFDVTEVYYDLIRDNPLTPEREDG
                     LNSAEAIEWIQKRHTESWFLKYRLTSIDKQTTNEAGEAVFSTVQVTEEAPSSRDKVYL
                     FLETYSPAHISRIASPMVVMMPVMMPDMVDGVWDGSTWKDTYNTDVHLYPKNEIREAD
                     KQMNVEESDLRQVTIINEAGEQETISYIDLERGKTASYTITAPIPYFIDSVLENGSAV
                     IKNYKITDTPTVGLTYYDQEIEVRAGETILTKGQDYIVEVVSNGFVVTILTEENGVAK
                     VDTLGRLADARGGDLTITYNLKVSTELEADDFHNNTAVIEIGRNDEFDYEEGVEPPEK
                     VTTGGRKFEKYDASSSELLKDARFELWNEDRSEYAIFYKGESPLAVYESGADRIEWAT
                     SGQATEFVADGNGYFEVQGLDYGTYQMKETMAPEGYVLPTGEAAFTEFIISYGSYNEE
                     IQIVGVENPGPERVPNMKRGSLPATGGNGLLAFLLIGISLMIGAYSWYRKSKMKSEV"
BASE COUNT         5684 a         4189 c         3445 g         6155 t
ORIGIN      
        1 tctttccctc ttttttttgt ttattcgaat tctttttctt ccagacggaa taacggactc
       61 atcggtgtat tttcatggat gcgtttgacc gcttcaccca ttaattctga acatgtaacg
      121 attgttaagt tttctggatg acgattttct tctgtgaaaa cagaatcagt gatacaaata
      181 tctttgattg gtgcattgtc taaaatactt ttcgcatgat cggacaataa tccatgtgac
      241 gcacaggcat atacttcttt tgcgccgttt tcttttaata catttgctgc tcgtgcaagc
      301 gttgctcctg tgttcaagat atcatctacc atgatacatg tctttccttg aacatcaccg
      361 atcacataac caccctcagt tgaagcatca tctgcatgat ctacgatggc taatgtgctg
      421 ttcaaatatt ctgaaagact acgtgcacgc tgaacaccgc tgttttttgg cgaaacaact
      481 acgatgtcat ctcccgtcag acctaattga cggtaataat gagcaaacaa cggcatcgta
      541 aataaattat ctacaggaat atcaaagaat ccttgtactt gaaccgtgtg cagatctaaa
      601 gtcagtactc gagttgcccc tgcttcttcg atcaagttag cgatcaattt cgcagtgatc
      661 ggttcatgag gtttcgccgt gcggtcttgc ctagcatacc catagtaagg caatacgaca
      721 ttgatcgttt tcgcacttgc tcgcttcatt gcatcgatca tgatcagtag ttccatatag
      781 tagtcattga caggtgcatt cgttgattga acgatataca catgatcccc tcgaatactt
      841 tcttcgatat tgatggaaat ctcgccatca ctaaattgct tcacgacgct tttacctaat
      901 tctgttccga acactttcgc gattttttca gctaatggac ggttgccatt caaactaaaa
      961 atcttcaacg tgtcatcctg atacttatta ctcatgtagt cctccccaaa acttctctat
     1021 tcttcgatag tttaacatat ttttccaaga ttttctgtta atttcagcaa attcttaagg
     1081 aagtgaaact gttccgttac tccaactggc aggaatgact ttatgataat cgccccagtt
     1141 gtcgtgtact ttgtaataac gttctcctgt ttcagtttca aaataagcgt acgctgtcac
     1201 ccaatgattg ccatatgtac tgccaagtac tttcaaaata ccaatcatca caggtttgcc
     1261 ttcacgtata cgttttgtag ctcgctgcca tgaacctaga agagttgctc gtgcacgctc
     1321 atgattgcca ttcttcttta aaaaactaga aatccctgta ctaacctgaa aaggaatcgt
     1381 aggaagtcct agtggttgga tctggctacg caatgccttc gtcaaaacca acgaatcagc
     1441 cgtatttttc ttgcgaatct catttggaag catccacccc ttacgatagt cttgataata
     1501 ggcaaggaga actgctgccg cgtaagtacc acaaagatag cctgaggggg tagcccattt
     1561 cttatacctc aaaaaacgtt cttctggcaa tcccacccag caatttgcga gttcctttcc
     1621 tgaccttttt tcttcgatca aaacgggaat atcttctttt ctgttgatcc atctagataa
     1681 ctcgattggc tcttcattga tcagcttcca ttttttacaa gaaagaccct cttgataagt
     1741 atattttgct ggagaatatt ctaccggatg tcctgattga tcgataatca cataactttg
     1801 atcttcaaaa accagagcta aaaaaagcaa tttacttttt cttgactgca gctgctgagt
     1861 gctggctaat tttttaattt ttttatctac tttgaaatat aaaggtttat ccactacttg
     1921 attcagttgg gatcgcctcc tttttttcat tataagtgaa ggattgaaaa aaacaaatca
     1981 gtttgattgt tcgaattttc aattttttat gttctatttg ttaattcctt cattttgtaa
     2041 gataatgggc acacgaaaca aaaaaggagc gatcgatgaa tgaacaaaga acgattgaaa
     2101 caagatttct atgaagcagt caatgaagaa tggcaaaaaa cagcaaaaat ccctgcagat
     2161 aaaccagcta caggcggttt ccaagattta gttgacggga tcgaccaact tttgatggga
     2221 gaaacagacg atctgcttgc ccatccagaa aaagtgtctt cagaaaaaat gaaacatttc
     2281 cttgcatatt atcaactggc aaacgattat gaccgaagaa atgagttgca tgctcaaccg
     2341 cttcaaccta taatcgaaaa agttgaatca ttgcagtctt tcgacgaatt gaataagcaa
     2401 tttccagaat ggctgttgga tacattacca ttgccattta atctggatgt agatgcagat
     2461 atgaagaatg cgcaaacgaa cgccttcttt gcttatccgc cttctttatt ccttccagat
     2521 aagacgtatt atgcatcaga ccatcccaac ggtgcacaac taataggcgt tttctttgat
     2581 atggctgttc agttattaga gcttgccggc aaagaaaaaa gtcaggcaga agcaatcacg
     2641 gaacaagcag tgcaatttga taaattgatt gctcctcatg taaaaagtgc agaagaaaac
     2701 gcagattaca gcaaaatgta taatccgcaa tcttttgata catttattaa atacacagat
     2761 aaactggact tgaaaaaact tgtcacgggt ttgattggga caactccaga taaagtgatc
     2821 gtaactgatc cagtgtactt cgatcatttg aatgacatcc tgacagaagc tcattttccg
     2881 ttattcaaaa gctggatgat cattaaaaca gtacgttctc ttagtggtta tctatcagaa
     2941 gaattccgcc aagtcagcgg aatctattct cgcgtattgt ctggaacaga cgaagcaatg
     3001 cctcagaaaa aagcagcgta ttaccttgcc tctggacaat tcgatcaagt agttggcgat
     3061 tactacggaa aaaaatattt tggcgaagct gctaaaaaag acgtagaaca aatggtgaaa
     3121 aaaatgatcg ccgtttataa aaaacgtcta gaagaaaatg actggcttgg ggatgcaaca
     3181 aaagaaaaag ccgtcataaa attaaataac ttaggtatcc aagtaggcta ccccgataag
     3241 atcccagcat tattcgacca gttcaaaaca gtacctgcag atcagggtgg tactttgctt
     3301 tcaaatactc ttgttttcag caaactcaca ttaaaagatc gtttcagcaa atggaacaaa
     3361 ccagtggatc gttcagaatg ggaaatgagt gctgacacgg tcaatgctta ttatcatcca
     3421 tttcggaatg tgatcgtctt tccggcagcg atccttcaag caccatttta cagtctagaa
     3481 caatcaagct ctgctaatta tggtggaatc ggagctgtta tcgctcatga gatttcccat
     3541 gcatttgata ataacggcgc tttatttgac gaatatggca acttgaacaa ctggtggaca
     3601 gaagaagatc ttacacaatt tcaaaacaaa gcacaagcaa tgatcgaaga attcgatggt
     3661 ctgccttttg ctgacggaaa agtaaacgga aaattaaccg tttcggaaaa tattgctgat
     3721 gcaggcggtc taagctgtgc attggaagca gcaaaaacgg aatcagatct ttctatcgag
     3781 gaattctttg tcaattgggc aacgatttgg cgtacaaaag caaaaaaaga ataccaacaa
     3841 cttttactgc aaatcgatgt ccatgcccct gctaaactcc gagcaaacgt gcaaccaaaa
     3901 aaccttgacg aattctatga aacatttggt atcacggaac aagacgctat gtatctagcg
     3961 ccagaaaaaa gagtgcgaat ctggtaagag gttgtgccag aagtggtcag cttcaagaaa
     4021 taagaaggaa tttgggaaaa tcgcttctca aatttttgtg acatttcggc ttatttttcg
     4081 aagaagttac ttctggaata ccatgtatta gaggttgtga aaaagctgct tagtttcaag
     4141 tagtaagaaa aattttgtga aaatggcttt ccacattttt gcataaattt gacttactac
     4201 cgagaaactg gcttttgaac accgtttatc gagaggttgt gaaatcagcg tcctgacctg
     4261 agtattagag aaaaaaacgg aaaaatagtt cctcatattt tgccgtttgt tgagctaata
     4321 ccgagggtca gctgattgaa caccgtttag tggagagatt gtgaaaaagc tgtcctgcat
     4381 caagtaataa gaacagccaa tcaaaagcag caaggagaac ctcatctata aaaagataga
     4441 aggaagtgag acatctattg gtctcatttc cttctatttt tactttcctc agtcaattgt
     4501 tttacatcat aaaaggagaa attccactta tttttttcgc tgtttcatga cataaacaac
     4561 tacaccgatt aacaaaattc ctccagctaa tgctagcaac gggttattaa attctcccgt
     4621 ttttgggagt ccttgagtgc catttttttc actttctttt gctgtcctat ccggatgaga
     4681 aggttttcca tttggttttc catttggtga ttcatttcct ggattttctc tttctggttt
     4741 gtcatttccc gggggcacat cggcagtttc tcgcacagtg atcgcttttt gctgggtaac
     4801 agattctgta tgagcagacc atttttcccc atcaaatatt tccgaacgat aagtgacttc
     4861 tagcgtatat tctccttttg aaggtgctga aaaaatagcg gtatgattag aaaattgagc
     4921 acttgactgt gtttcaacga tttggatctt ttctgggaaa taacgcatat cttcaaagac
     4981 aggcgttcct tgatttggtg tgaaagttga cacaaaaact gtaaaaggct tcagttcaat
     5041 gatatttcct tctgcattct ccgccacacc atttgcttct tgtataataa tcggattatt
     5101 ttgttcattt ggttttgtct gatttggttc ttgtacgatt gcatactctg gatattccga
     5161 agacggtgag gtttctccgt caaaaatagc acctacagat aatggaagac catccactct
     5221 ttccttttct gtttgacgat tccccggttt aatctgcatc agtccatgaa catggatact
     5281 tccattttca tagcgcgtaa caggctgctg accttctttc gaacatgtat ttagagactc
     5341 gatccaatct tttgatatgg tatgtccaag agtattttct gattgtttct ctcgttcatt
     5401 ccaatagtag ttcaacgagt ggtagatttg atcttcgttt tgattcaatg ggcggatctg
     5461 ctctctcatc tcaagattct ctgttcggaa agataagata ccagaagcat ggtagtctgt
     5521 gtttcctgct gaagaggtat aaagagccac attatatgaa ccgttattaa aacttgtact
     5581 gctattgacg ataatatctg ttccgctatt ggaatcgatt cctttcgcaa gattttcgaa
     5641 agacaaacta ttgatcaatt catgagggcc cgttaaagaa gaaccgccca ttttgaatcc
     5701 attaccgtta ccttctccct caattcctgg tacccagccg tttcgataag caactgagtt
     5761 tttgatgatt acttttccga tcgaacctgt ttcattttta gcaaaaaggt cccaaccgtc
     5821 atctgcatta tgatacgcga tacaaccgtc aaatacattt ccttctccta ttgtcagttt
     5881 ggccgcaaat ccatcggcat cttcaaatcc tggatcggca ttttcaaaag atgtacagtt
     5941 cttgactaag ttatgcgcgg gccatgcttc gaaaggatca tttgagctac cgctgatttg
     6001 gattcctgta tttccattcc gataagcatg aatctcttcg atcaaattat gatttccaga
     6061 aatctgtaat cctttttgca tagctgcact atttgtaatg tcaaaccctc gtaaacccca
     6121 gtagtgactg aacagtgtca tcccatttcc agttccttta aaatcaagta caggacgctt
     6181 gttttctgga tttttctctg caatcaactg aatcgggttt tctcttgtcc cgttaacgcc
     6241 ttttggaatc gtcaatcctc tttcgaagga ataagttcca ggcgccatga cgattgtctg
     6301 cccagcatat gcgtatctca atgcttgtgt cagagataat ggattttgtc tgcttccgtt
     6361 tcctgcatct gacccttctg gtgttacata aactgtcgaa tatttaggtt tacggtagtc
     6421 cactgtatga agaatctctt ttgtttcata actagtcatc tctacgtatt cttctggcgg
     6481 atagttttta tctggcgtaa aactgaaagt aaattcgttc gttccttcca tcagttttgt
     6541 ctgataatca aattcccgat tcgcagtgat agccaagttt tttgcgttct taagcacctc
     6601 tcctgttgct ttatttttaa ttgtcaataa tccgtcagca tttgctctaa aggacatagt
     6661 atgatcttgg tttcctgaat gattactaga ggtcacagtg taaatcggat caatcaagcg
     6721 agtaggtctt tcttgcccag gaggatctgt cgcaggatct gagtaagcaa cttcgattcc
     6781 ttctaccttg atcgtcatat tccttgctgc agcaaatcct gcataaataa ccgattcatc
     6841 tgaagcgtat aatttttccc agtcatacat gatgtcagaa gccatttctt ctcctgtttc
     6901 aggatcgtaa taatatgact ctaaaccagt attcgttttt ttcatctcta cgtccagttt
     6961 agtaatcgag ttcgtggccg gaaacaaatt cccatgaccg tttccgaata tattataaga
     7021 acctgtttct ttttccaaga accctgctga agtctctaaa ggtgttgttt ccactgagac
     7081 actcccagga gctggcgcag cttgcggatc ttcagatgat attcctgtga ttgcccgtgt
     7141 ccccaatcca agtcgcatcg tatatttgct gccgctcgtg ttgaccactt cttgtctatc
     7201 cgagtcccag acatattcga tccgactgcc tagtatggca aatgaatttg aataaaatgc
     7261 ctgcccaaac aaatgatttt ttggtactgc gtctcttacc attaaagcaa atccttcttg
     7321 ggcattcgta taggtccaag attctatttc gatcgtcgcc cgcatcctaa agttgatgtc
     7381 tttggggatt gcttgataat aataggtcat tccatctgat ccgcttgact ggaattttcc
     7441 gccgttattc gtggaagaga gccgcacgcc gtcttctaat ggttcaatgg catacctact
     7501 agcacttgtg ctttctccga aaacaacccc tttccactct tcatttgctt cttcagattg
     7561 cttttttgtc ttttcatttt ctgattcttt catcatagga tcatttcctt cttccgtatc
     7621 catatcttca ctagttcttg gtacttcctc cattgttgac atcccttcaa tgttcacgtg
     7681 tgcattttct tcgttcacag actgttgttc gttagcagca acttcaacga cagaaattaa
     7741 aaccggggta ctgagcataa ggagattcat cattagcgca ctgtatttta tacgtttttt
     7801 taacaagtgt attcctcccg taacaaatta gaaagcgttt acttaaagta ttacctaatt
     7861 tatttttact gtcaataaaa aacaaaatta taataagtgt attttataaa aagaaaaaat
     7921 ataacttaag ttgttgataa taaagagatt tattaacgta aaaagaataa gtttttttaa
     7981 aataacgtat aaaaaagagc ataaaacaaa gtcgtttgtt ttatgctctt cttttattat
     8041 tttgtgcttt ttattttatc aaatcacttt gtttttttat aaaatttctg cgcctagtgt
     8101 ggactcaaaa tgtctcaatg cccattcatg tccaacggga tcgaaacttg ccacggcatc
     8161 tttgaaaata tggagtttgt atcctagatt atacgcatcg actgctgtat gcaacacaca
     8221 gatatctgta catacgccgg tcaaataaat atctgtgatc tggcgttctc gcaaacggat
     8281 gtctagatct gttccactaa aagcggaata atgccgtttg tctatccaat aaacattttc
     8341 ctcttgctca tagtcttggt ataaactttt taaagagccg tacaattccc gtccacccgt
     8401 tcctatcaca ttatgcggag gaaataagcg gttctccgga tgaaaggaat cttttggatc
     8461 atgcgcatca atggcaaaaa cgacaaaatc cttttgatca ataaacttct ttgtatgagc
     8521 agtcagcttt ttttcaatta cttgtcctgc tgtcccagta gtcagctttc catcctctgc
     8581 aacaaaatca tatgtgtagt caatcgagat caatgcgttc atcttagtcc tccttatacg
     8641 agatgtttga caaattcgat caccatttct gctgatcgtt tgcctgcgtc ttcgataaat
     8701 tcgtcaaagc tttgtgtcgc cgagtgatct gctgtgtcgc tcattgcacg tacgatcaaa
     8761 aatgggatat tgaactgacg agctgtttgt ccaacagcgg ctccttccat ttcgcaagct
     8821 aatgcttcag ggaaattcgt taagatttct ttgattttat ctggcgagtc gacaaatgta
     8881 tcacctgtaa cgatcaatcc ttcttttgcg ttcaaattcg ttgcttttgc tgctttaacc
     8941 atttcactgc gcaaatatgt gcttgcttca taatatagag gcattcctgg taattgtcct
     9001 ggtttgtagc caaatcctgt tgcatctgca tcaaaataag ctactttgtc tgatataacg
     9061 atgtcaccta cttgtaagcc ttcaccgatt ccgccagctg aacctgtatt gatcaccatg
     9121 ttcacgccgt attgttggat caacagactt gtggtaatag atgctaagac ttttcctata
     9181 ccggaacgta cgacgatcac ttcgtggtta cctaatgaac ctgaaataaa taaagcgcct
     9241 gcgcgttccc atgataatgg ttcgcttagg ttctctctca aaatttttac ttcttcttcc
     9301 attgcgccga taataccgat tttcatcttt ttaattcctt ctttctatat aaatgcgatt
     9361 gctaaaaaaa ccacacataa cattaggata acaataataa gagatttcat caaaaaagag
     9421 ttccatttgg tcgtcttttc ccgttcgctg atccttgttt tcgtgattgg tttattcttt
     9481 ttgctttctt tacgataaaa gctggcaatc tttttttctt cttgctgata agctgtttct
     9541 gcttttcgct gtttttttaa tgactcgctt gcttgtgctt gttgtcgctt gcgaagttcg
     9601 cttcgcgtaa taagcggtcc tttggccata agccgatccc tctactttcc tttggtcaat
     9661 agttcccagt attgaatcgc ataaatcgtc tttgcatcac aaatcaactg atctttcatc
     9721 gcttgttgcg cctcttcaag agttagatga tacaactcca gtacttcgtc ttcatcttgt
     9781 gctaaaggat tctcgacctt ctctacccct tgtgcatgat aaatatgaag gacttcattt
     9841 gcaaaacctg gtgaaagata catcgaagta agatggctca gcgacttcgc acgatagcca
     9901 gtttcttctt ctaattctct agctgctgtc atttcgggat tttgtccttc tcctggatcg
     9961 atttttccag ctggaatctc caaaatcact ttttctaaag gttttcgaaa ttgtttgaca
    10021 agaagcagac gatcttgttc gtcaaaagca atgatcccga caccaccagg atgaaatacc
    10081 agttctctct tcgaagttcc tccatctggc aaacgaactt cgtctacagc aacatcaata
    10141 atttttccgc tatagatttc tttgcgactg attgtttttt cttcaaattg ctcattgttc
    10201 agcatatctt ctcctgcttt ctttgtaatc ataactgctt ttttttcatt tttcaatttt
    10261 tctcaaaaac tccttattag tttaccatag ataaacctta gattttctct aaatatagaa
    10321 aatccttctt tagtccgata ctctttctag tagtccgtat gctaaaataa gtatgtaatc
    10381 gaataacgaa aagaattcaa tactcaacga taaatgacat ggagggttta ttatgataaa
    10441 acgaaattgg ccgtggcttc tagcattggt cttgctattt ctctttgtcc gaaacaatag
    10501 tttttctttt gttctaggac ttttattgat aggtgtagtt ttagttttgt ggggactctt
    10561 tggttcgcgc aggaaaaagg gaaaacaaga agaaattcct gcactgactg acgaacttga
    10621 aactcactat gaaaaaagcg gtatgacagc gaatgaaatt tcattttttc gacaaacaat
    10681 gaatcaaaca aaaaatgaga tcaatcaatt gcaaatgaat atgcagcaga cagccaaact
    10741 taaagcaatc gatctccgcc atgatccagt caaagcagcg aaagctttat tcaaagaatt
    10801 ggtgaaagaa ccaaaccgac tgcatgaagc aagtcagttt ctttatacgc atctgccgaa
    10861 tctagttgat ttgacaaata agtataatga gatcaatgac catgagatca aaaacaaaca
    10921 gacctatgaa aaactagaag aaagcgcgca gatcatcgac cagctgtcag ctttaatcgc
    10981 acaagattat caaaaattcg ttgctgagga tctagatgat atcgacgtag agatatctgt
    11041 tgcgaaacag agcttaaagc gtgataataa atatgaacca gaacaaaagg attaactctt
    11101 ttgttcataa aagaagactc ggaacattca gatgatgtct ggctgtttcg agtacttttt
    11161 ctaaagtaga gacattcatg taagaatcga gaacagattc aacaaggagg aattttttat
    11221 ggatcataaa ccgactgaaa caaaagatgt tacaccagtt aacgatacac ttgaagattt
    11281 attgaacaac ccgttttcaa ctccaacaga tacgctgacc acttcacaac aagcggagat
    11341 tgatcagctc caagagcaac agacagctgc tcgtttaatt gacaaactac cggcggaacg
    11401 ccaagaacaa gctaaacagc tagcagcaaa aatcgatgct aatgatgcac agtctgtcat
    11461 cagctacgga tcggctgccc aagcaaaatt aagtgaattc tcacaatcga tgttgaatca
    11521 tgtgcaagca caagatattg gtcctgtagg agattcctta actgaactga tgtatcgtct
    11581 ccaagaagcc aatcctgacg aactgcgtgc cggagaaggg aacatcttcc agcgtgtctt
    11641 cgggaaggtc aaacaatcga tctatgaagt gacagcaaaa tatcaaaaga ttggtgcaca
    11701 aattgacaag atttcagtga aattagataa agaaaaagac ggtctgctaa aagataatct
    11761 gatgttagaa cagctttacc agaaaaacaa agactacttt gatgcactga atatttatat
    11821 cgctgctgga gaattgaaaa tggaagaact tcaaacaacg atcattccag aagcgatgaa
    11881 aagagcagaa gaaactggcg atcagatgga tgttcagatt gccaatgatt acacgcaatt
    11941 cctagatcgt ctggataaac gtacgcatga tcttcgctta gctagacaga tcacgatcca
    12001 gcaagcacca caaatccgtt tgatccaaaa cacaaatcag gccttggctg aaaagatcca
    12061 agcatctatc gctacagcca ttccgctgtg gaaaaaccaa gtcgtgatcg cattgacctt
    12121 gcttcgtcaa aaagatgcag taactgctca acgccaagtc tctgaaacaa caaacgactt
    12181 attgaagaaa aattcagaaa tgctgaaaat ctcagccatc gaaacagcga aagaaaatga
    12241 acgtggaatc gtggatatcg agacccttca aaccactcaa aatgatttga tcgaaacaat
    12301 ccaagagaca ttgcgtatcc aaaaagaagg aaaagaaaaa cgtcgtcatg cagaagtaga
    12361 gcttggacac atggaagaag atctgaaaca aaaactgttg gatttaaccc aatagatcac
    12421 ctttcgcttg ttactaaaac aaacgcccaa caacagttta ttacaactgt tgatgggcgt
    12481 ttttcttatc tttcttctaa cttctccatt ggagtctatt tctcattcat cttgctgtgt
    12541 ccatagccat agccaaaata aatgatcagt ccaataacca ttacgatacc gaacacgatc
    12601 catgtcacag tttgcagacg aagcatcaat acaatacaag tcaagatgga gacgattggc
    12661 aatagcggaa cgaatggcac tttgaattct ccagcttttg gtttgccaaa catttttctc
    12721 aacttcagta agcctagact aacaagcatc aaataggcga gcgccatgat attcgttagt
    12781 tccgctaata cattcaatgg gaaaaatccg gcaaagaata cagctgccac accagcggca
    12841 taagtcgctt ttttcggtgt acgatgcttt tgatcgattt ctgtcataaa cttaggaagt
    12901 aagccatcct tgctgacagc aaacagtaaa cgagcaagtg aatacatcat cgagatcgtt
    12961 accgtcagta gcgttaaaat tgcccctaca gcgatcaccg cgcccactgt tccatgtcct
    13021 acatacctca ttgcaaaagc cactgggtca ttgactccta gttcagtata cggaacgatt
    13081 cctgtcaaga tcagcgtcac gatgatgtaa aggacagtag caatcagtat cgaaccaatg
    13141 attcctcttg gcacatcttt ttgcgggttt ttgacttcct cggctgccat tgcaacggca
    13201 tcaaatccca agaaagcaaa aaacacaaga gctgcaccat ctagcacacc ggaaaaacca
    13261 aaaggcatga atggctgcca gttatcaggt ttaacgaaaa atattcctac aatgataaac
    13321 aacgcgatga taccaaattt gacatatacc atggcattat tcaaacgtaa tgctttcttg
    13381 gcttcaagcg acacgatgta agtgacgaat atcactacga gcaccgcaat caagtcaatg
    13441 tatgtaccat tttccggatt gtacccgcca gttaatgctt gtggaagttc ggcccctaaa
    13501 cttttcaaaa aaccttgcat atagccagac catccggaag caacagagga aaccgctagt
    13561 aaaaattccc cgatcaacaa ccaccctgtc agccaggcag caaattcacc aaatacaaca
    13621 tacagatagg catatggccc accaatcact ggcactcgcg aagcaaattc tgcgaaactt
    13681 agtccagaca aaatgacaac gattgcagct aagacaaatg aaagtgacag tgctggacca
    13741 gcattttgat tggccgcaac ccctgtcacc acaaatattc ccgtaccgat gatcgcacca
    13801 attccaagca tgatcaaatc agacgttttt agttcttttt tcatctcact cgtatgattg
    13861 agattgatct ctttcttacg aaacaaatcc attgaagttc ctccccattt taggttggaa
    13921 aacagcgatt aaactgtttt catataccct aaaaggatac cacctaaaat gactaaaagg
    13981 caaccaacaa taacataaat gagctctttt ttcgtttttc tttcgccaag gatgaaaatc
    14041 ccacctaaag tagagataat gatccccatt tgtgaaagtg agaagccaac agctaaacca
    14101 agtgatttga ctgttagaag catgcagatg tttcctaagc cccataataa tccacagatc
    14161 atgtttttcc acacaaactg atcgacttta actttacgga aagcaaagaa gcttgcgcct
    14221 aagatcattc cgatactttg aggtaagata attgacatcg catccaaatt cgcccagttg
    14281 acgatgatgg tatatacacc ataaccaatc gtagagaaga tcaatgctct aaaaccctta
    14341 ccaaaatcaa gcattttatt atctgtatct atttttcctt cgtcatcttg tcgggcagtc
    14401 aaatacgcac cgcttacaag aagagctaaa gcaataatac cgtatacata atctttcgtt
    14461 tgtgtccatt catggaagaa aacggctccc gcaatcgtat tcaaaattaa ttgaaaacca
    14521 gtagacaatg gtagccctac agaaacaccc atgtacttca ttccatggaa ctgtccattt
    14581 tgtccaacac tccaagctaa gccggataaa aatccgatca agaaaatcca aggggtgatt
    14641 acaggactaa caacgaaaaa gacaactaaa gaaaacagaa atgcaccgat cgtcattcct
    14701 agtgtttgtt gattggcatc gccaccaatt ttaccactta ctaaaccaat actaccccat
    14761 gccaacatag ggactaatgc tattaaaatt tccataacca atctcctttt atttcacatg
    14821 ataagaaact tatcagcgaa aacgttaaca acacaagcac tttttttcgt ttctaacgat
    14881 tttctaacgt ttttcattat aaaaatttaa gattgggaaa aataaagtca ttgttgtccc
    14941 ttttccttca gttgattcag ccgtcaaatg gatacctaat ttttcggcca gattttttgc
    15001 taaataaagt cctaatcctg ttgaatgtgt tttgctaagt cgaccatttc tcccagtaaa
    15061 tcccttatca aagatccgtt tcaagtcttc cgctgggata ccgatccctg aatctttaac
    15121 tgaaaggctg actccttgcg cttccttttc tatgctcaca atgatgtttc cgtgatccgg
    15181 cgtatatttg atggcattac taagcaattg gttgaagata aaagcgatcc atttagcatc
    15241 ggtcaaaaca gtatgatcct caccaacgat ctcgtagcga atattttttt ggataaaata
    15301 atttgcctga ctgcgtatca caggctgaat gattcctttt agtccatatt cttggatgag
    15361 gtagtcccgc gagaaactgt cgagtcttgc ataatacaaa acttgttcta cgtattcatc
    15421 gatttttgat aattcatttt ctagtaatgt atacttttga tcgtcaatat cgaactcgat
    15481 cgaacgaact agcaatttag aagcggaaag tggcactttg atttcatgga cccatgagtc
    15541 aatataatct ttttggtctt gttgattact gattgctgat tgcattactt cttggtgttc
    15601 acggatcagc tgattgatat attcttctac cagtttttct tcttctgtcc tcccaccact
    15661 caaataattt tgcaaagggg aagttttttg tatgtcggct aattttcccc accatcttct
    15721 cttgctcaag taaccaccca gcagataaaa aatcagaaag acgccctcaa tcaaagctaa
    15781 ataagcaatt gtgctcaaat caactttgat gtttggcgcc agccacatca caaagatcgt
    15841 caatgcaata aaaaacagcc atcccaagat caatgtccac tgatctttca aatatttaaa
    15901 aaaattcatc ttaaccgctc atcctttcag ggaacgatat atccctgtcc tacttttgtt
    15961 tcgatatatc cttggatacc tgcctgttcg atttttcggc gtaaccgatt gatgttgact
    16021 gtcaatgtat tgtcatctac aaatcgttcg tcatcccaca gtgctcgcag caatttttcc
    16081 cgacttaaga tctttccatt gtgcttcatc aaaataaata atagacgata ttcatttttg
    16141 ctcaaatcaa tgatctctcc gttgatttcc atacttccgt tgtcaacatt caacgtaatg
    16201 ccattgtggc tcatcatttc actagataat tctgtgtagt tgtaagaacg gcgaagtaat
    16261 gcattgattt ttgcaatcag tacatcgatt tggaaatgtt ttgtcacaaa atcatctgcc
    16321 cccatgttca tagccatgat catatccata ttcgtattcc tgctggagat aaaaatgatc
    16381 ggtactttcg atacttcacg tattttttga caccaataat agccatccaa aacgggaaga
    16441 ttaatatcta gtaatacaag ctgtggttct tctctttcaa agtctgagaa cacttgattg
    16501 aaatcggtcg ttccaaaggt ttcaaattgc catttctcaa gttcctccat gatcaattgt
    16561 cgaatgatct cttcgtcttc gactaccatg atttttgcca taaaaaatcc ctccatcttt
    16621 cttcttgcta ctacaatatc aaagaagccc ttgtgaaaca agttggctta cctggaaaat
    16681 gtcattttag caaacgttaa aaaaaactgg aaatatgcga tttaatcgcg tttgtctctt
    16741 ccaagcagct taaatttatt atgttattct taataattgg tctattatct gttatttttc
    16801 tttcgttgtt tttgtctttt aaattttcga taacaccaat aaccgaaaag cgttagcact
    16861 agcaatatac ctagaaggag tataagtaat cgataaagat ggtatttttt tgtctgttct
    16921 atctttgaag agacttcttc tgtaaaggga actcgtcttc ctgtcactag taatcggtga
    16981 gtgttcacgg tgtaaggtgt acaagtgata agtgtgacta gatcctgctt ttctctaatt
    17041 gctaatgtat ttgtttttgt tggaaggaca acaattttct caacgacttc ataagcgagc
    17101 atccgaccat acacttcaag atagaatcgg tcatcttgct ccatttcttc aagatccgta
    17161 aagagttttt tctcagcaat accgctatgg gcggtaatca cggaatgtgt actctctcct
    17221 ccagttggaa aagaggtgcc ttgcaggact gtcgctcctc tatctaacaa aagatcattc
    17281 gtttcatcaa ataaaggcaa gctaacattg attttcggaa tatagatggc tccgatcata
    17341 tgttgttcgt aatattcttt tcctggattt tggacatttc taaccgcttg ctcaaaagga
    17401 tctttgactt gtcccattcc tgggattact gatgtatgag caagcgtctg atttttcttt
    17461 tcatgcactg ctaattgttg ctcatgtttt tcttctttat cgcgttcaag ctgcttttga
    17521 taattctcga tccttctttg atccaaataa ttgctgagag catctgctaa aaatggatat
    17581 gtaaaaactg cagctccggc aagaaacatc actgccataa ataattgtaa taaaagactg
    17641 atttgggttt gaggtctatt acctattttc tttttcattt tcttcacctt cttatttaaa
    17701 aaaactgcag caacctttat aaaaccgatt gctgcagctt atttttgttg cttatacttc
    17761 agacttcatt ttagatttac ggtaccagct gtacgctcca atcatcaaac ttattccgat
    17821 tagtaagaat gccaagagcc cgttcccacc agtagcagga agtgacccgc gtttcatatt
    17881 cggtactctt tctggccctg gattttctac tcctacgatc tgaatttctt cattatagct
    17941 tccataagag atgataaatt cagtaaaggc tgcctcacct gttggaagta cataaccttc
    18001 tggcgccatg gtctctttca tttgataggt tccataatct aacccttgaa cctcaaagta
    18061 tccatttcca tctgcgacaa attctgttgc ttgtccgctt gttgcccact caattctatc
    18121 tgctcctgat tcataaacag ctaaaggcga ctccccttta taaaatattg cgtattcaga
    18181 acggtcttca ttccacaatt cgaatcttgc atctttaagc aattcactgc ttgatgcatc
    18241 atatttttca aactttcttc cacctgttgt gactttctct ggcggctcta ctccttcttc
    18301 ataatcaaat tcatcatttc gtccgatttc aataaccgct gtgttgttat ggaaatcatc
    18361 cgcttctagt tctgtggaaa ccttcaaatt atacgtgatc gttaaatctc ctccacgtgc
    18421 atctgctaat ctacctaaag tatctacttt ggctactcca ttttcttctg tcaaaatcgt
    18481 cacgacaaac ccgttgctta ctacttctac tatataatct tgtccttttg ttaaaatcgt
    18541 ctcgccggct cgcacttcta tttcctgatc ataataagtt agaccaactg taggcgtatc
    18601 tgtgattttg taatttttga taacagcgct tccgttttca aggacagaat cgatgaaata
    18661 aggaataggt gctgtgatcg tgtaagaagc cgtttttcct ctttctagat caatataaga
    18721 aatcgtttct tgttctccag cttcattaat aatcgttact tgtctaaggt cgctttcctc
    18781 tacattcatc tgtttatctg cttctctgat ttcgtttttc ggatacaaat gcacatctgt
    18841 attgtatgtg tctttccatg tagaaccatc ccacacgcca tccaccatat caggcatcat
    18901 cactggcatc ataacaacca ttggagacgc gattctggaa atatgagctg gcgaataagt
    18961 ttctaagaaa agatagactt tatctctaga agaaggtgct tcttccgtca cttggacggt
    19021 agagaatacc gcttctccag cttcattcgt tgtttgtttg tcaatacttg tcagtcgata
    19081 ttttaaaaac caactttctg tgtgccgttt ttgaatccat tcaatcgctt cggcagaatt
    19141 aagaccgtct tctcgttccg gtgttaaagg attatctcga atcaaatcgt aatatacttc
    19201 cgttacgtca aacaaatcaa aatcgactcc tggaatattt tcttcaccaa aatccgtaga
    19261 aaccaatccg ctattcggtc gttcttccgg aacagatgag aaaccttttt tgtgtaatgt
    19321 aatcgtcact tcacttggtt tttgtgcgac ttcttcggct ttgactagtt ttccgttccc
    19381 gaaaatccct attcccgcag taacgagtaa cagtacactc attcccgtga caatcatttt
    19441 tttattcatt tttttctccc cgttcttttt tta
//
LOCUS       DACEEZ010000050        19457 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20313_2017
            SAMN12639966-rid9879223.denovo.050, whole genome shotgun sequence.
ACCESSION   DACEEZ010000050 DACEEZ010000000
VERSION     DACEEZ010000050.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639966
            Sequence Read Archive: SRR10023158
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 19457)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19457)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741683.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741683.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:44:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,016
            CDSs (total)                      :: 2,962
            Genes (coding)                    :: 2,889
            CDSs (with protein)               :: 2,889
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 36 of 73
            Pseudo Genes (incomplete)         :: 29 of 73
            Pseudo Genes (internal stop)      :: 18 of 73
            Pseudo Genes (multiple problems)  :: 8 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19457
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639966-rid9879223.denovo.050"
                     /isolate="E20313_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            133..3660
                     /locus_tag="IWO89_001796"
     CDS             133..3660
                     /locus_tag="IWO89_001796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase"
                     /protein_id="HAQ0456529.1"
                     /translation="MSLQFILGNGKKDHRQVLIDEASAWLDQDASNQVFFLVPNYSKF
                     EQEQEILAEMRRRSGKKTFSTTNVQVFSFYRLAWYLLQQTTLLTGNELSESGSAMILR
                     QILEKNEEELTIFRGEINKRGFIRQLQELYSELRTGNIQPEDLMLLFGDSPNAEDQQL
                     KMKDLKLIFSAYDSELTQRALTNEDALSILANYMQTQDFNNVKFIVSGFSRINGQEYQ
                     LLQAMMEKGQLVVDLLLDRPYTADLPEVLTLFHETGKLYFRLFQSARERKVPILVDRF
                     APEQTRPTELQLLQTMWEETSSQKKAAVHKIGGQEHLHLWEAENPTEEVRQLAVEIRR
                     LVSEENYRYRDIQVLTKNMSLYGNILRPIFQEMAIPFYIDEEQMMENHPLIEWINSLF
                     ALDRYSYRLSDIMRFFKTELFLSTEAETELDEWKQERDEFRKKLDITENVALAYNYQG
                     TYWTREKDWQFVAYDFEAEQIEDVQYLEEQSNTIRRLFQKAVPVFLKKIKKSKTGQEA
                     ANVFYQFLIQSGVQQQLLFWRNQEVDRGNLEAARNHEQTWGALIDLLDEYVVIYGESA
                     FDWSLFQEIITSGLENLSYGKIPTAIDQVRINRLELVRPNQAAVTFALGLNDQVFPDR
                     KETKGLLSSEEREVLNQALPDGQFLFDPSKESISFEPFQAYLVFLSGTDRLYLSYAQS
                     YDTDGSLKMSPYLRRITEYLSVPVERKEHLTIESDPNCYVGTYRSGINTVNRIYRLAL
                     DEKKPVPKHWQKLKELVLNSSWRNFALQIFESQEHLNVPVSMPEKMAEELYGKEIYTS
                     ISRMENFYNCEYKYFMQFGLKLKERTVYGLTPAATGDFYHESLDRFFKLLFSNQLSLV
                     NMSDAQRRDFTEKVLQEVFGELRFEILDSSARMNYIRYQLGQTIQKVTWALQKQSKNT
                     GMHPLQTEILFGQIAGAKGITGLELPLSNGGKLHVRGKIDRIDVASEQEDTWLSVVDY
                     KSSGRSFDVTEAYYGMAMQLLTYLDVALMDAVRLTGKAAVKPAGSYYLHVHNPVLTET
                     DDVEKNTLKKFSYDGVFVDDPALLEVLDHSLEAKEQSLIYPVKKNAKDEYQKGSFSKD
                     KFFTEEELQLFMAHNRENMRLAGDAITSGEIKLNPAYKDKQRIACEFCPFRSICTFDV
                     MLKENNYHRLEKIDKEEALRRILKRSEEE"
     gene            3657..7379
                     /gene="addA"
                     /locus_tag="IWO89_001797"
     CDS             3657..7379
                     /gene="addA"
                     /locus_tag="IWO89_001797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase-exonuclease AddAB subunit AddA"
                     /protein_id="HAQ0456530.1"
                     /translation="MNQIPLKPQNERFTDDQWQAIFDQGDNLLVSASAGSGKTTVLVR
                     RVIEKLKMGFDIDELLIVTFTEAAAREMKERIQEALQESVNSESDPVRRQHFTKQLVL
                     LPTANISTLHAFCLTVIRRYYYLIDIDPVFRMLTDETETILMKEDVWDELREALYAEN
                     DERFFQLTMNFSNDRSDDGLTNLVFSLYEFARANPDPQKWLEQLSDNYRLPEGLAKSR
                     LYQKQIRPLVLADIYQCVQLYEQMTQLAQGEGLEKMHEQVAGEQQQIKNIYEAFSQDR
                     LEEAYAGLEQLTFSTFKSSRKAELKEISNEVKGMRDKAKKLIQQISKSYFPVSPSQME
                     ELTDKALPLVEEMTKVTQSFMDGFSMRKREKGVLDFNDLEHLALQILTEKTKDVWLPS
                     EASKHYRKKFKEVMVDEYQDVNQLQEAILYWLREPDDTKGNMFMVGDVKQSIYSFRLA
                     DPSLFIGKYENFSKKEGGRRIVLAENFRSRKEVLSFTNLIFEQLMDPAVGQINYDEAA
                     KLIQGFSDFPENEQFEPEIMIYEKEQEESEIEIPTDDILEDKTEGELFMTGLKIRQLI
                     DSSFMIYDKKSKKSRPIEYKDIVLLTPTKKNNLTILEIFKTLDIPLEMNDAQNYFQAT
                     EIRTMISLLQLIDNPYQDIPLAAVLRSPIVGLIEPELASIRLANRAHTYYDAVLAYQA
                     SNEDELAAKLEHFGKQLEHWRELARRSSITDLLWDIYYETGYLEYVVGLPAGAQRQAN
                     LYALVDRAKAYEQSSFRGLYQFVRFIEKMQEKDKDLAEPVISIEDNAVRVMTIHASKG
                     LEFPVVFLLDMTKEFNLQDLRNRYAFEEKLGAGIRYMDPETRVLYDTLPFQAIKLAKQ
                     NKLLSEEMRKLYVGLTRAEQKLFIVGSYKNKEQMIQTWSEAADHEELVFDPALRLKGR
                     SSLMNWIGYGLIRHPEMQKYLEEEISTSLLQHSNAQFSISWMNQQSIIEQRQLLAEKE
                     LVNLDQQMKEDETLLADSLQKRLAYEYPYQASSQTTSYQSVSEIKRLFEDPDDTQESR
                     LTLESSQNKAASRQFRYTQEQLAEPKFLQKDRQVSAAAVGTATHALLQLLPLEMPTTE
                     SIHQKLQELVRKRLVDEKVAKKVDVSSIIWFFQTELGQQLIANKENVKREQPFSMLLP
                     ADEVFQDYPNQEDELLIHGIVDGYLEEKDHLNIYDFKTDFILHPDDPAEIDAIVQKYQ
                     GQLRLYQQAMSEALNKPVENVFLILLRVKQIININK"
     gene            7448..7789
                     /locus_tag="IWO89_001798"
     CDS             7448..7789
                     /locus_tag="IWO89_001798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAQ0456531.1"
                     /translation="MELTQTEFVLCPKFEKTFSILGKKWNGLIIDVLLENGPQRFVEL
                     AAKIPMVSDRVLVERLKELEKEGIVTRNFACSEKSRSEYLLTEKGADLQKAMRQIQVW
                     SEKWITEEECG"
     gene            8098..9141
                     /gene="pheS"
                     /locus_tag="IWO89_001799"
     CDS             8098..9141
                     /gene="pheS"
                     /locus_tag="IWO89_001799"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HAQ0456532.1"
                     /translation="MSLQAQLESLKEATLVKITAAQDLNELNQIRVETLGKKGPITEV
                     LRGMKDLSPEERPKVGSFANEIRDLLTEEIERKKQVLEEEALNAALAKETIDVTLPGR
                     KVKHGTRHIVTQIMEEIEDIFVGMGYQIVEGYEVESDHYNFERMNLPKDHPARDMQDT
                     FYISDEILIRTHTSPVQARTMEKHDFSKGALRMISPGKVFRRDTDDATHSHQFHQIEG
                     LVVDKNITMGDLKGTLEVVMKKMFGEDREIRLRPSYFPFTEPSVEVDVSCFKCGGAGC
                     NVCKYTGWIEILGAGMVHPNVLKMSGIDPEEYSGFAFGLGPDRVAMLRYGVNDIRNFY
                     QNDLRFLSQFKGE"
     gene            9146..11566
                     /locus_tag="IWO89_001800"
     CDS             9146..11566
                     /locus_tag="IWO89_001800"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HAQ0456533.1"
                     /translation="MLVSYKWLNRYVDLSNVTPKELADKMSVTGIEVEGVTVPEEGLK
                     KIVVGEVKECVPHPDSDHLSICQVDIGEDELSQIVCGAPNVKVGIKVIVALPNSRIAG
                     NVKIKKGKMRGQVSNGMICSLQEIGYSDSVIPKEYAEGIYYMPQEAVNGDPVFPYLDM
                     DDSIIELSITPNRADALSMRGVAYEVGAIYRQTPTFDDEKLVESSRPASDKIQVEVED
                     KEAVPAYQIRIIEGVRIQPSPQWLQNLLMNEGIRPINNVVDVTNYILLLFGQPLHAFD
                     YDKLKSKEIFVRRAKENETLVTLDGESRELTTDNLVITNGKEPVALAGVMGGLDSEIT
                     NETTTVALESALFDPISIRRTAKQFNLRSESSARFEKGINHATVGEASEVAAAMIVSL
                     AGGEVLQGAVKGSEIKAEDVSVKISLERINQYLGTSLVVKEVNEIFEALGFSYEEKEY
                     VYSVVVPPRRWDIQIEADIIEEVARIYGYDQLPSTLPSGETVAGSLSKEQETTRKIRT
                     IFEGSGLSEAISYALTTEEKSRQFVINESNITRLDWPMSEERSVLRMNLISGLLDDIS
                     YNTARKNTEIALYEIGRVFFQENDPRTHLPQEVKHAAIAISGIWEEKDWQTKAQNVDF
                     FTIKGLLENLFEQLGITEDVHYQTVTEMKEMHPGRTAAIYLGEKFIGFVGQVHPTITK
                     SYDIPETYVAEFDLSAIVEAAQKGIVFEAVTKFPAVSRDIALLVKETVTNQELTEVIR
                     SAAGRFLTDIQLFDVYQGENIEKGHKSMAYSLTFVNPEATLTDEEINKAMEKVEKALV
                     ENLEASIR"
     gene            complement(11736..12746)
                     /gene="trpS"
                     /locus_tag="IWO89_001801"
     CDS             complement(11736..12746)
                     /gene="trpS"
                     /locus_tag="IWO89_001801"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HAQ0456534.1"
                     /translation="MKNIILTGDRPTGKLHLGHYVGSLKTRVAMQADEDNQLFVMIAD
                     MQALTDNAKNPEKVSSNVLQVALDYLAVGLDPAKSTLFIQSQIPELAELTMYYLNLVS
                     VGRVRRNPTVKTEIEQKKFGESVPTGFFIYPVSQAADITAFKANLVPVGEDQKPMLEQ
                     TQEIVQSFNHTYGEVLVEPKGVFPPKGMGRLPGIDGNGKMSKSLGNGIYISDPADVLQ
                     KKVMSMYTDPNHIHVQDPGQVEGNMVFTYLDVFGTDKEAIEEMKAHYRRGGLGDVKIK
                     RYLIDVLEAEFAPIRARREELEKDPSAVMEILRKGSEEAAKVAAQTLSEVKSAMGINY
                     FH"
     gene            13000..13737
                     /locus_tag="IWO89_001802"
     CDS             13000..13737
                     /locus_tag="IWO89_001802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HAQ0456535.1"
                     /translation="MAMIKFDHVDKYYGKFHALKNINLEFEKGEVVVVIGPSGSGKST
                     MLRCINGLETISSGKLLINDTDLHDKKTKLTEVRKNIGMVFQHFNLYPNKTVLENIKL
                     APIKVLKQDQATAVKNAEKFLKTVNMLDKKDSYPSMLSGGQQQRVAIARGLAMNPEML
                     LFDEPTSALDPEMIGDVLDVMKKLAHDGMSMIVVTHEMGFAKEVADRVIFMADGEVLE
                     DSRNVRDFFENPNEVRAQQFISKVINH"
     gene            13751..14566
                     /locus_tag="IWO89_001803"
     CDS             13751..14566
                     /locus_tag="IWO89_001803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HAQ0456536.1"
                     /translation="MRRLKKIRLIFLLGIAFLALAGCKGNGLANEDILERSKSTNEII
                     WGVKYDTRLFGMMDIESRTVQGFDVDIAKAITKKILGDNGKTEFVEVTSKTRIPLLKN
                     GNIDAIIATMTITDERKKQVDFSDVYFDAGQALLVKKGSQIKSVDDLNASTTVLAVKG
                     STSAANIRQHAPDAKILELENYAEAFTALQSGQGDAMTTDNAILLGIADENPEYELVG
                     GTFTNEPYGIAINKGQENFLKAVNQALEEMHADGTYDKIYQKWFPNETEGKVE"
     gene            14579..15226
                     /locus_tag="IWO89_001804"
     CDS             14579..15226
                     /locus_tag="IWO89_001804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HAQ0456537.1"
                     /translation="MSELIQTYGPIFVDGFKITLFSSVLALVFSLIIGTLMAIFQLSK
                     NKVVSALAKAYVEFFRNIPLLIIVMFFYVVVPIYWFNLDGFQAGTIGLTIYTSAFIAE
                     TVRSGIQTVPKGQTEAGLSSGFTYSEIMRYIVLPQAFKIVIPPLGNQFINLVKNSSIL
                     AIVAGLDLMYQGDLIASETFNTFDTYIIVGLFYLVITLPLSYLMVYLEKKWAVRS"
     gene            15242..15898
                     /locus_tag="IWO89_001805"
     CDS             15242..15898
                     /locus_tag="IWO89_001805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HAQ0456538.1"
                     /translation="MDFSGAFSWMNIRFLLEGLKVTVEVSLFSIIFSFLIGGLTGVIR
                     FANIPFFSKILGVVIDIIRNLPLLLIIFFTYFALPQIGIQMNIFWSAVAALTIFESAM
                     LSEIFRAGLNAVPKGQMEAGLSTGLTYVESMRTIVLPQAFKSMIPAIVSQLISLIKDT
                     SLAVIISLPELSHQARIIYGQNTNYVLPMFIIMTLMYFVVCYALSLLSGYLEKRRYSY
                     "
     gene            16060..16881
                     /gene="racE"
                     /locus_tag="IWO89_001806"
     CDS             16060..16881
                     /gene="racE"
                     /locus_tag="IWO89_001806"
                     /EC_number="5.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HAQ0456539.1"
                     /translation="MIRLTDNRPIGFIDSGVGGLTVVKEALKQLPNENILFVGDTARC
                     PYGPRPAEQVIQYTWEMTDYLVEQGIKMLVIACNTATAVALEEIKAALSIPVIGVILP
                     GTRAAVKKTQNKQVGIIGTIGTVKSQAYEKALKEKVPELTVTSLACPKFVSVVESNEY
                     HSSVAKKIVAETLAPLTTKKIDTLILGCTHYPLLRPIIQNVMGENVQLIDSGAETVGE
                     VSMLLDYFNLSNSPQNGRTLCQFYTTGSAKLFEEIAEDWLGIGHLNVEHIELGGK"
     gene            16884..18239
                     /gene="rph"
                     /locus_tag="IWO89_001807"
     CDS             16884..18239
                     /gene="rph"
                     /locus_tag="IWO89_001807"
                     /EC_number="2.7.7.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease PH"
                     /protein_id="HAQ0456540.1"
                     /translation="MRHDGRKAQELRKLELQTNVFKYPEGSVVIRFGDTTVICSATIE
                     NSVPPFLRETGTGWVTAEYSMLPRATSTRNRRESSKGKLSGRTMEIQRLIGRSLRAVV
                     DLEKLGERSIIVDCDVIQADGGTRTASITGAFVALKLAIEKLLREKELSEDPIKEHLA
                     AVSVGILPDGTCVTDLDYQEDSAALVDMNLVMTESGKFVEIQGTGEEATFDGEQLNEM
                     LFFGKNAIEDLIKEQKHALLTEFAQNDERIEETKTIIIATRNPGKAEEFRNMFKEAGY
                     HVKTLLDYPELPDVEETGSTFEENARLKAETIAQLLDQPVLADDSGLKVDALGGMPGI
                     YSARFAGEQKSDAGNNAKLLYELTDVPDEKRTAQFHCTLVFAAPKKDSLVVEAEWPGR
                     VARIPSGENGFGYDPLFIPEGKKQTAAELSSEEKNKISHRAQAMKKLSAEWKQWLEGE
                     R"
     gene            18240..18758
                     /locus_tag="IWO89_001808"
     CDS             18240..18758
                     /locus_tag="IWO89_001808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HAQ0456541.1"
                     /translation="MRYLVVSDNHGDRDILVDLVDRYRSEVDIFFHCGDSELEASDPL
                     WDTFQVVQGNCDYGPGFEQKKVIQTGQDTVFMTHGHLSNVRFGLTQLAIEARAATADM
                     VFFGHTHQIGCEMDQKILFLNPGSISQPRGPIQIPSYALIESLDDQISVQYYNRAHQA
                     VEELAFSFSRER"
     gene            18865..19365
                     /locus_tag="IWO89_001809"
     CDS             18865..19365
                     /locus_tag="IWO89_001809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HAQ0456542.1"
                     /translation="MIGPIVREMLLENQETFLIPAENVANVMYHHPLSHGLLVLSKVG
                     YSKIPVLGKDDHFVGLVSLSDVVNKMIDLQTISMDPLEGLTVADVTETDVPTIDENWE
                     LEEVLHLLVDSPFLPVVTEDRIFKGIITRKELLKAVNFMVHELERRNILSPKIEELKE
                     QIDIVS"
BASE COUNT         6541 a         3341 c         4178 g         5397 t
ORIGIN      
        1 tttttgcgta tacttaaaaa agtagaaata tcagaacctt aaaaggatat aatttgaaga
       61 atatagaaca aatgttcagt attttttgat ataataaagg agcaccgtat catgtgttga
      121 aggaggaaaa gcatgtcttt acaatttatt cttggaaacg ggaaaaaaga tcaccgtcaa
      181 gtcttgatcg acgaagcatc cgcttggctc gaccaggatg caagtaacca agtatttttc
      241 cttgttccca actacagcaa attcgaacag gaacaagaaa ttttagcgga gatgcgaaga
      301 agaagcggaa aaaaaacttt cagcacaaca aacgttcaag tatttagttt ttatcgtctg
      361 gcttggtatt tgctgcaaca gacaactctt cttacaggaa atgaattatc agaatcagga
      421 tctgcaatga tcttgcgaca gattttagaa aaaaatgagg aagaattaac aatctttcgc
      481 ggagaaatca ataaaagagg gtttattcgt caattacaag aactctacag tgaattgcgt
      541 acaggaaata ttcaaccaga agacctgatg cttttatttg gtgattcacc taatgcagaa
      601 gaccagcaac ttaaaatgaa agacttgaag ctgatttttt cagcttatga cagcgaattg
      661 actcaacgtg cactaacaaa tgaagatgca ctatctatac tagcaaatta tatgcagaca
      721 caagatttca acaacgtcaa atttattgtc agtggttttt ctcgaatcaa tggtcaagag
      781 tatcaattac tgcaggcaat gatggaaaag gggcaattgg ttgtcgattt attgctggat
      841 cgtccttata cagcggatct tccagaagta cttacgctct ttcatgagac tggaaagctt
      901 tatttccgtc tttttcaaag tgcaagagaa cggaaagtcc ctatattagt ggaccgattt
      961 gcaccagagc agactcgtcc gacagagctt caactgctgc aaacaatgtg ggaagagaca
     1021 tctagccaaa agaaagcggc agtacacaaa atagggggac aagagcatct ccatttgtgg
     1081 gaagctgaaa atccaactga agaagtccga caattagctg ttgaaatcag acgactagtc
     1141 agtgaagaaa actaccgtta tcgtgatatc caagtgctga caaaaaatat gtctttatat
     1201 ggaaatatcc ttcggccgat ctttcaagaa atggcaattc ctttttatat cgatgaagaa
     1261 caaatgatgg aaaatcatcc gctgatcgaa tggatcaaca gtctgttcgc tcttgaccgc
     1321 tattcttatc ggctaagtga tattatgcgt tttttcaaaa ctgaattatt tctgtcgacg
     1381 gaagcagaaa ccgaacttga tgaatggaaa caagagcgag atgaattcag gaaaaaactg
     1441 gacatcactg aaaatgtagc acttgcttac aattatcaag gaacttattg gactcgtgaa
     1501 aaagactggc agtttgtggc gtatgatttt gaagcagaac agattgaaga cgtacagtat
     1561 ttggaagaac agtcaaatac gattagacga ttatttcaga aagctgtacc agtatttttg
     1621 aaaaaaataa agaaaagcaa aacaggacaa gaggccgcaa atgtttttta tcaatttttg
     1681 atccaaagtg gcgtacagca acaattattg ttttggcgta atcaggaagt agacagagga
     1741 aacttagaag cggcaagaaa ccatgagcag acatggggag cgctgattga tctgttggat
     1801 gaatatgtag taatttatgg tgaatcggct tttgactggt cactgttcca agaaatcatt
     1861 acaagtggtc tagaaaatct aagttatggt aaaatcccta ccgcaatcga ccaagtgcga
     1921 atcaatcgtt tggaattagt tcgaccaaat caagctgccg tcacttttgc attagggttg
     1981 aatgatcaag tgtttccaga ccgaaaagag acaaaaggat tgttgtccag tgaagaacga
     2041 gaagtattaa atcaggcctt gccagatgga cagtttttat tcgacccatc aaaggaaagt
     2101 atctcttttg agcctttcca agcctatctt gtttttcttt ccggaacaga tcgattatat
     2161 cttagttatg cacaaagcta tgacacagat ggttcgctga agatgtctcc ttatttgcgt
     2221 agaatcacag aatatttatc tgttcccgtc gagagaaaag agcatttgac gatcgaaagc
     2281 gaccctaatt gttatgtagg gacttacaga agcggaatca atacagtcaa taggatttat
     2341 cgattagctt tagatgaaaa aaaacctgta ccaaagcact ggcaaaagct caaagaatta
     2401 gtactgaatt cgtcatggcg aaattttgct ttacagattt ttgaaagtca ggaacattta
     2461 aatgttcctg tttcgatgcc agaaaaaatg gcggaagagc tttatggaaa agagatttat
     2521 acttcaattt cacgtatgga gaatttttat aattgtgaat acaaatactt catgcaattt
     2581 ggtttgaaat tgaaagaacg aactgtttat ggtctaactc cagcagcaac tggtgatttt
     2641 tatcatgaat cattggatcg tttcttcaag ctactgtttt caaaccaatt atccttagtg
     2701 aatatgtccg atgcgcagcg tagagacttt actgaaaaag tgctgcagga agtattcgga
     2761 gaacttcggt ttgagatatt ggatagttct gcaagaatga actatatccg ctaccagtta
     2821 ggacagacga ttcaaaaagt gacatgggct ttgcaaaaac agagcaaaaa tacaggcatg
     2881 catcctcttc agacagagat tctatttggt cagattgctg gagcaaaagg tatcacaggt
     2941 ctggaattac cgttgagtaa tggtggaaaa ctccatgttc gagggaaaat cgatcgaatc
     3001 gatgtagctt ctgaacaaga agatacttgg ctaagcgtcg ttgattacaa atctagcgga
     3061 cgttcattcg atgtcacaga agcttattat ggcatggcta tgcaattatt gacgtatctg
     3121 gacgtagcat tgatggatgc ggtacgtttg actggaaagg cggctgttaa accagcagga
     3181 tcatattatt tacatgttca taatcctgta ttgacggaga cagatgatgt agagaagaat
     3241 acattgaaaa aatttagtta tgatggcgtt tttgtcgatg atcccgctct tttagaagta
     3301 ctagatcatt cactggaagc aaaagaacaa tcactgatct atccagtgaa aaaaaatgcg
     3361 aaagatgaat accaaaaagg tagtttcagt aaagacaaat ttttcacgga agaagaactg
     3421 caactattca tggcgcataa tcgggaaaat atgcggttag ctggagatgc gattacttcc
     3481 ggagaaatca aactaaatcc agcttataaa gataaacagc ggattgcctg cgaattttgt
     3541 ccattcagaa gcatctgtac gtttgatgtg atgctgaaag aaaataatta ccaccgtctg
     3601 gagaaaatag ataaggaaga agcactgcgt agaatattga aaaggagtga agaagagtga
     3661 atcaaattcc tttaaaaccg caaaatgaac gattcacaga tgatcagtgg caagcgattt
     3721 ttgatcaagg agataatcta ttagtctctg cgtctgccgg atcaggtaaa actacagtgc
     3781 ttgttcgaag agtgattgaa aaactaaaaa tgggttttga tatcgatgaa ttattgattg
     3841 tgacatttac tgaagctgcc gcaagagaaa tgaaagaaag aattcaagag gcattacaag
     3901 aatccgtcaa tagcgaaagt gaccctgtac gtagacagca ttttactaag cagttagttt
     3961 tattgcccac agcgaatatc tcaaccttac atgctttttg cctgacagtt attcgacgtt
     4021 attattactt aattgatatt gatccagtct ttcgcatgtt aacagatgaa acagaaacga
     4081 tcttgatgaa ggaggatgtg tgggacgagc tgagagaagc actttatgca gaaaatgatg
     4141 aacgattttt ccaattaaca atgaattttt ctaatgatcg ttcagatgac ggcttgacga
     4201 atctggtgtt ttctctttat gagtttgccc gtgccaaccc tgacccccaa aaatggctag
     4261 aacagttaag cgataattat cgtttgcctg aggggctagc gaaatctcga ttatatcaaa
     4321 aacaaatccg tcctttagtt ttggcagata tataccaatg tgtacagtta tatgaacaga
     4381 tgactcagtt agcacaaggt gaaggattag aaaaaatgca tgagcaagta gctggagaac
     4441 aacagcagat caagaatatc tatgaagcgt tctcgcaaga tcggttagaa gaagcttatg
     4501 ctggtttgga acaactaact ttttcaactt ttaagagcag tcggaaagca gaattgaaag
     4561 aaatttccaa tgaagtcaag ggcatgaggg ataaagcaaa aaaactgatt cagcagataa
     4621 gtaaaagtta ttttcctgtt tccccaagcc aaatggaaga attaacggac aaagcactac
     4681 cgttagtcga agaaatgaca aaagtcacgc aaagcttcat ggacggtttc agtatgcgta
     4741 aacgggaaaa aggtgtgctg gatttcaatg atctggaaca tttagcgttg cagattctca
     4801 cagaaaaaac gaaagatgta tggctgccaa gtgaagcttc aaaacattat cgaaaaaaat
     4861 tcaaagaagt aatggtagat gaatatcaag acgtgaacca attacaagaa gccattttat
     4921 actggctaag agaacccgat gatacaaaag gaaatatgtt catggttggg gatgtcaaac
     4981 aatccatcta ttctttccgt ttagcagatc ctagtttatt tatcgggaaa tacgagaatt
     5041 tctcaaagaa agaaggcgga cgtcgaatcg ttttggctga gaacttccgc tctagaaaag
     5101 aagtcctttc ttttacgaat cttatttttg aacaactaat ggatccagca gtgggtcaaa
     5161 tcaattatga cgaagcagcc aaactgatcc aaggattttc agactttcca gagaatgaac
     5221 agtttgagcc ggaaatcatg atctatgaaa aagaacagga agaatcagag attgagattc
     5281 cgacggatga tatactggaa gacaaaacag aaggcgaact ttttatgaca ggactaaaaa
     5341 ttcgtcaatt aattgattcc tcctttatga tttatgataa aaaatcaaaa aaaagtcgtc
     5401 cgatagagta caaagacatt gttctcttga cacctacaaa gaagaacaac ttaacgattt
     5461 tagagatttt caaaacattg gatattccat tagagatgaa tgatgctcaa aactattttc
     5521 aagcgacaga aattcgcacg atgatctcgc tgctacaatt aatcgataat ccctatcaag
     5581 atattccgct tgcggctgta ttgcgctcac cgatcgtggg tctgattgaa ccagaattag
     5641 ccagcattcg attagctaac cgtgcacata cttactatga tgctgtgctt gcttatcaag
     5701 catcaaacga agatgaatta gcagctaagc tggaacattt tggcaagcaa ctagagcatt
     5761 ggcgagaact tgctcgccgt tcttcgatca ctgacttgtt atgggatatc tattatgaga
     5821 cgggatatct agagtatgtg gtcggtctgc cagcaggggc acagagacaa gccaatcttt
     5881 atgcattggt tgatcgtgcc aaagcttacg agcaaagttc tttcagagga ttataccagt
     5941 tcgtgcgttt cattgaaaaa atgcaagaaa aagataaaga cttagcagaa ccagtcattt
     6001 caatagaaga taatgctgtt cgcgttatga cgatccatgc aagtaaaggc ttggaattcc
     6061 cagttgtatt tttgttagat atgacaaagg agttcaactt acaagacttg cgcaatcggt
     6121 atgcttttga agaaaaactt ggagcaggaa ttcgctatat ggatcctgaa acacgagtgt
     6181 tatatgatac gttaccgttt caagccatca aattggcaaa gcaaaacaaa ttgttgtcgg
     6241 aagaaatgcg taaattgtat gtaggattga ctcgggcaga acaaaagctt ttcattgtcg
     6301 gttcctataa aaataaagag caaatgatcc aaacgtggtc tgaagcagct gatcatgagg
     6361 agttagtttt tgatcctgcg ctacgtttga agggcagaag cagcttgatg aattggattg
     6421 gttacggatt gatccgtcat cctgaaatgc agaaatactt agaggaagaa atttcgactt
     6481 ctcttcttca acattcaaat gcacagtttt ctatttcatg gatgaatcag caatcgatca
     6541 ttgaacaaag acagttgctt gctgaaaaag aactggttaa cttagatcag cagatgaaag
     6601 aagatgaaac tttattagca gattcgttac aaaaacgtct ggcatatgaa tatccttatc
     6661 aagcttcttc acaaaccaca agttatcaat ctgtatctga aatcaaacga ttattcgaag
     6721 atccagatga tacacaagaa tcacgtttga ctctggaaag cagtcagaac aaagcagcat
     6781 cccgtcaatt tcgttatacg caagaacaac tagcagaacc aaaatttttg caaaaagatc
     6841 ggcaagtgtc agcagctgct gttggaactg ctacgcatgc tttgttacaa ttactgccat
     6901 tagagatgcc aactacagag agtatccatc aaaaattaca ggaattggta aggaaacggt
     6961 tagtagacga aaaagtcgcg aaaaaagtag atgtttcttc tattatatgg ttctttcaaa
     7021 cagaacttgg tcaacagctg attgccaaca aagaaaacgt aaaacgagaa caaccttttt
     7081 ccatgctgct tcctgctgac gaagtatttc aagactatcc aaatcaagaa gatgaactat
     7141 taatccacgg aatcgtcgat ggatacctag aagaaaaaga tcatctcaat atctatgatt
     7201 ttaaaacaga ttttatttta catccggatg atccagcaga aatagatgca atcgtccaaa
     7261 aatatcaagg acagctaaga ctttaccaac aagcaatgtc agaagctttg aataagccag
     7321 tagaaaatgt tttcctaatt ttgcttcgag tgaaacaaat tataaacatt aacaaataat
     7381 tataattgag caaacttgac ttatgttttg taaggattat gctatactct gggaagaggt
     7441 gagtatgatg gagctaacac agacagaatt cgttttatgc cctaaatttg aaaaaacatt
     7501 ctctatttta ggaaaaaaat ggaacggatt gattatcgat gtactactcg aaaacggtcc
     7561 gcaacgcttt gttgagcttg cggcaaaaat cccgatggtc agtgatcgag tattagtcga
     7621 acgcttgaag gaattagaaa aagaaggaat agtcactcga aactttgcct gtagcgaaaa
     7681 gagccgttca gaatatctct tgactgaaaa aggagcagac ttgcaaaaag cgatgcgtca
     7741 aatacaagtt tggagcgaaa aatggatcac agaggaagaa tgcggttgat tttgtaaaaa
     7801 aaatcttgta aactatagtc catacgaata agtaaagaac gatgacgaaa aagagtagtt
     7861 tggctaaacc agttcaggga gatctgtcgc agactgaaag cagatcctgt gtttgaaaaa
     7921 tgaagttcac tttccgagtt cgtctgataa aatcagaccg gcaattgtcg ttaacgaagt
     7981 aagagtgcgg tgtaagtcat ttatccgcaa attaggatgg taacgcgaag acctcgttcc
     8041 ttaacaggaa tgaggtcttt ttgttttgta tttggaattt aaaggaggaa caagcacatg
     8101 tctctacaag cacaattaga gagcttgaaa gaagcaacgc tcgtcaaaat cacagcggca
     8161 caagatttaa atgaattaaa tcaaatacgt gttgaaacgt taggaaaaaa aggaccgatc
     8221 acagaagtat tacgtgggat gaaagatcta agtccagaag agcgaccaaa agtcggaagt
     8281 ttcgcaaatg agatccgaga tcttttgaca gaagagatcg agcgaaaaaa acaagtatta
     8341 gaagaagaag cgttaaatgc agctttggca aaagaaacaa tagacgtgac actacctggt
     8401 agaaaagtaa aacatggtac acgtcatatc gtgacacaaa tcatggaaga aattgaagat
     8461 atttttgtgg gtatgggcta tcagatcgta gaaggatatg aagtagaatc tgatcattac
     8521 aatttcgaac ggatgaatct gccaaaagat catccagcaa gggacatgca agatactttc
     8581 tatatttcag acgagatatt gattcggaca catacttcac cagtccaagc acggacaatg
     8641 gaaaaacatg atttctctaa gggtgctcta cgaatgatct cgccagggaa agtcttccgc
     8701 agagatacag atgatgcgac ccacagtcat cagttccatc aaattgaagg gctagttgtt
     8761 gataaaaaca tcacaatggg cgaccttaaa ggaacattag aagtcgtaat gaaaaaaatg
     8821 tttggggaag atcgtgaaat tcgtttgcgt ccaagttatt tcccatttac ggaaccatca
     8881 gtagaagtag atgtcagctg tttcaaatgt ggtggtgccg gttgtaacgt atgtaaatac
     8941 actggatgga tcgaaatttt aggagctggc atggtacacc cgaatgtgtt gaagatgtca
     9001 ggaatcgatc cagaagaata ttcaggcttt gctttcggcc ttggaccaga ccgcgtagct
     9061 atgttgcggt atggagtgaa tgatatccgt aatttctatc aaaatgactt acgtttctta
     9121 agtcagttca agggggagta agaaaatgtt agtttcatat aaatggctca atcgttatgt
     9181 agatttatcg aatgtaacac caaaagaatt agcggataag atgtctgtga caggaatcga
     9241 agtagaggga gtaacggttc cagaagaagg cttgaaaaaa atcgttgtcg gtgaagtcaa
     9301 ggaatgcgta ccacatccag attctgatca cctttctatt tgtcaagtcg atattggcga
     9361 agacgaactg tctcaaatcg tttgtggagc gcctaatgtg aaggttggaa tcaaagtgat
     9421 tgttgcactt cctaactcac gtatcgctgg taatgtaaaa atcaaaaaag gaaaaatgcg
     9481 tggccaagta tcaaatggca tgatttgctc attacaagaa attggctatt cagatagtgt
     9541 tataccgaaa gaatacgctg aaggtatcta ttatatgcca caagaagctg taaatggcga
     9601 tccagttttt ccttatttgg atatggatga ttcgattatt gaattatcta tcacaccgaa
     9661 tcgtgcggat gcattgtcaa tgcgtggcgt agcttatgaa gttggggcaa tttatcgtca
     9721 gactcctaca ttcgatgacg aaaagctggt agaatcctca cgtcctgcta gcgataaaat
     9781 tcaagtggaa gttgaagata aagaagcagt tcctgcttat caaatcagaa tcattgaagg
     9841 agtaagaatc cagccaagtc ctcaatggct gcaaaatctt ttgatgaacg aaggcattcg
     9901 tccaatcaac aatgtagtag atgtgacgaa ctatattttg cttttattcg gacaaccact
     9961 acatgcattt gactacgaca aattgaaatc aaaagagatc tttgtccgcc gtgcaaaaga
    10021 aaatgaaaca ttagtaacac tagatggaga atctcgggaa ttgacaactg acaatctcgt
    10081 cattactaac ggcaaagaac cagttgcttt agctggtgtc atgggagggt tagactcaga
    10141 gatcacgaat gaaacaacaa cagttgcact agaatctgct ttatttgatc caatttcgat
    10201 tcgtcgtaca gcaaaacaat ttaatttgcg tagtgaatcc tctgcacgtt ttgaaaaagg
    10261 aatcaaccat gcgactgttg gagaagccag tgaggttgct gcagcaatga tcgtttctct
    10321 tgcgggtggg gaagttctac aaggcgcggt caaaggctca gagatcaaag cagaagacgt
    10381 atcagtgaag atttcacttg aacgcatcaa tcaatattta ggaacatctc ttgtcgttaa
    10441 agaagtaaat gagatttttg aagcacttgg atttagctat gaagaaaagg agtatgttta
    10501 ttccgtagta gtaccaccaa gacgctggga tatccaaatc gaagcagata ttatcgaaga
    10561 agttgctcgt atttatggat acgaccagtt gccatctaca cttccaagtg gagaaacggt
    10621 tgctggaagc ttgtcaaaag aacaagaaac tactcgtaaa attcgtacaa tctttgaagg
    10681 aagcggattg agcgaagcaa tcagttatgc attgacaaca gaagaaaaat caagacagtt
    10741 tgttataaat gaaagtaata ttacacgttt ggactggcca atgagcgaag aacgttctgt
    10801 attacgaatg aatttgatca gcggactatt agatgatatc agttataaca ctgcccgaaa
    10861 aaatacagag attgctttat atgaaattgg tcgggtgttc ttccaagaaa atgatccacg
    10921 tacacatctg ccgcaagaag taaaacatgc agcaatcgct atttctggta tttgggaaga
    10981 aaaagattgg cagacgaaag ctcaaaatgt agactttttc accatcaagg gtcttttaga
    11041 aaacttattt gaacaactag gcatcactga agatgtccat tatcaaacag tgacagaaat
    11101 gaaggaaatg catcctggaa gaactgctgc gatttacctt ggcgaaaaat ttattggttt
    11161 tgtcggtcaa gtgcatccaa cgattacaaa aagttatgat attccagaaa catatgttgc
    11221 tgagtttgat ttatctgcta tagtggaagc tgctcaaaaa gggatcgttt tcgaagcagt
    11281 gacaaaattc ccagcagtca gccgggacat cgctttgctt gtaaaagaaa ctgtgacaaa
    11341 tcaggaatta acagaagtta tccgttctgc tgccggaaga tttttaacag atatccaatt
    11401 atttgatgtc tatcaaggag aaaacattga aaaaggacat aaatccatgg cgtatagcct
    11461 gacattcgtc aacccagaag caacattgac agatgaagaa atcaataaag caatggaaaa
    11521 agtcgaaaaa gcgttggtag aaaacctaga ggcaagtatt cgctaagaag gaaaatatag
    11581 taagtaacga tcaagtagct tttgaagacc atacatttga aaaaaagatg tgacacaact
    11641 tcgtcacatc tctttttttg attttaaaaa aggaatagca cagagacgag ataaaaaaac
    11701 aggcctttac agcctgcttt tttagagtaa atgaattaat ggaagtaatt gattcccatt
    11761 gctgatttta cttcagaaag tgtttgtgca gctactttag cagcttcttc actaccttta
    11821 cgtaagattt ccataacagc tgaaggatct ttttccaatt cttctcggcg cgcacggatc
    11881 ggtgcaaatt cagcttccaa tacatcaatg aggtatcgtt tgatctttac atcaccaagt
    11941 ccgccgcggc ggtaatgagc tttcatttct tcaattgctt ctttatctgt tccgaataca
    12001 tccaagtagg tgaagaccat attcccttct acttgtccag gatcttgtac atggatgtgg
    12061 tttggatcag tatacatact catgactttt ttctgtaaga catcggcagg atcggagata
    12121 taaataccgt ttcctaacga tttactcatt ttgccatttc catcgattcc aggtaatcta
    12181 cccattcctt ttggtgggaa tacgcctttt ggttccacca agacttcacc atatgtatga
    12241 ttgaagcttt gtacgatttc ctgtgtctgc tctagcattg gtttttgatc ctcaccaaca
    12301 ggtacaagat tcgctttgaa tgcagtgata tctgctgctt gagaaacagg atagataaag
    12361 aatcctgtag gcacgctttc accgaatttt ttttgttcga tttctgtttt gacagtcgga
    12421 ttgcgccgaa cgcgtcctac gctgactaag ttcaaataat acatggttaa ttcagctaat
    12481 tccggaattt gtgattggat aaataaagta gattttgcag gatctaatcc cacggctaaa
    12541 tagtctaaag caacttgcaa gacatttgat gaaaccttct caggattttt agcattatct
    12601 gtcagtgctt gcatgtcagc aatcatgaca aacagttggt tgtcttcatc tgcctgcata
    12661 gccactcggg tttttagaga accgacgtaa tgccctaaat gcagcttccc agttggtcga
    12721 tctcccgtta aaataatatt tttcaaaaaa gacacctctt tacgatttgt aagtctgagc
    12781 acttaatatc ttaccgtttt tcctgaaaag aaacaacagt tcctaaggaa aaaacaagga
    12841 aagaggataa aataactaat agattagaaa tggaagaaat atttatggga gaataaacgt
    12901 tctaagaaac tttacaaatt actaaactat cagatataat gtattttatt atacatcttt
    12961 aaaagaaatc ttactaaata ataatgggag tgtaattcta tggcaatgat taaatttgat
    13021 cacgtggata aatattatgg caagttccac gcattaaaaa atatcaatct cgaattcgag
    13081 aaaggagaag ttgtcgttgt cattggtcct tctggttcag gaaagagcac gatgcttcgc
    13141 tgtatcaatg gattagagac gatctcttca ggtaagctgc tgattaatga tactgacttg
    13201 catgataaaa agacaaaact gacagaagtc cgaaaaaaca tcggcatggt tttccaacat
    13261 ttcaatttgt acccaaataa aactgttctg gaaaacatca aattagcacc aatcaaagtg
    13321 ttgaagcaag accaggcaac tgcagtcaaa aacgcggaga aatttttgaa aactgtcaat
    13381 atgttagata agaaagattc ttatccttcc atgttatccg ggggacaaca gcaacgggtg
    13441 gcaatcgcca gaggtttagc gatgaatcct gagatgctgc tgtttgatga acctacttct
    13501 gctttggatc cggaaatgat tggtgatgta ttagacgtta tgaaaaaatt ggctcatgac
    13561 ggtatgtcaa tgattgtcgt gacacatgaa atgggctttg ccaaagaagt ggcggatcgt
    13621 gttatcttca tggctgatgg agaagtattg gaagacagca gaaatgtgag agactttttc
    13681 gaaaatccaa atgaagtccg agcacagcaa tttatcagta aagttatcaa tcattgatag
    13741 gaggaaaaag atgcgcaggt taaaaaaaat acgcctgatt ttccttttag gcattgcttt
    13801 tcttgcacta gccggatgta aaggaaatgg gttggcaaat gaagatattt tagagcgaag
    13861 caaatctacc aatgaaatca tctggggtgt caagtatgat actcgattat ttggaatgat
    13921 ggatattgag agcagaactg tacaaggatt tgatgtagat attgcaaaag caatcactaa
    13981 aaagatcctt ggtgacaatg gaaaaacaga atttgttgaa gtcacttcta aaactcggat
    14041 tccattattg aaaaacggaa atatcgatgc aattattgca acgatgacga tcacagatga
    14101 acgaaaaaaa caggttgact tttcagatgt ctatttcgat gctggccaag ctttgttggt
    14161 aaagaaagga agccaaatca aaagtgtaga tgatttgaat gcttctacaa cggttctagc
    14221 agtcaaaggt tctacatcgg ctgcgaatat ccgacagcat gcaccagatg caaaaatttt
    14281 agaattagaa aactatgcag aagcatttac tgctttacaa tctggtcagg gagacgcgat
    14341 gactacggat aatgcgattt tgttagggat tgcagatgaa aatccagagt atgagttagt
    14401 tggtggaacg tttactaatg agccttacgg gattgcgatc aacaaagggc aagaaaactt
    14461 cttgaaagct gttaaccaag cgctagagga aatgcatgca gatggcacat acgataaaat
    14521 ctatcaaaaa tggtttccaa atgaaactga aggaaaagta gagtaagggg gagaggcaat
    14581 gagcgaattg atacaaactt atggaccaat ctttgtcgac gggttcaaga tcacattgtt
    14641 ttcgagtgtc ttagcacttg tgtttagttt gattatcgga acgttaatgg caatcttcca
    14701 attatcaaag aataaagtag tgagcgcctt agcaaaggca tatgtagaat tttttagaaa
    14761 cattccatta ttaatcatcg tgatgttttt ttatgttgtc gtaccgattt attggtttaa
    14821 tctagatgga ttccaagcag gtacgattgg attaacgatc tatacatctg cttttattgc
    14881 tgaaacagta cgttcaggga ttcaaaccgt accaaaaggt caaacagaag caggactatc
    14941 ttctggtttt acctattcag aaatcatgcg ttatattgtt ttaccgcaag cattcaaaat
    15001 cgttatcccg ccattaggca accaatttat caacctcgta aaaaactcat ctattttagc
    15061 aatcgtagct ggtttagatt tgatgtatca aggagacttg atcgctagtg aaacattcaa
    15121 taccttcgat acgtatatca tcgttgggtt attttactta gttatcacgc taccgttatc
    15181 ttacttaatg gtttatcttg agaaaaaatg ggcggttcgt tcatagaagg aggggaaata
    15241 catggatttt agcggcgctt tttcatggat gaatatccgt tttttattgg aaggattaaa
    15301 ggttactgtt gaagtttcat tgttttcgat catttttagt ttcttgatcg gtggtctgac
    15361 aggtgtgatt cgttttgcga atatcccttt cttttccaaa atccttggtg tagtgattga
    15421 tattatacgg aatcttccat tattattgat cattttcttc acttactttg ccttacctca
    15481 aatcggcatt cagatgaaca tcttctggtc cgctgtcgca gcattaacga tttttgagtc
    15541 agctatgttg tcggaaattt tccgagctgg actcaatgct gttccaaaag gacagatgga
    15601 agcaggatta tcaacgggac tgacttatgt agaatcgatg cgaacgatcg tgttaccgca
    15661 agcatttaaa tcaatgatcc cggcaattgt gagccaattg atttcactga tcaaagatac
    15721 ttcattagca gtaatcattt ctttacctga actttctcat caggcacgta ttatttatgg
    15781 acagaacacc aattatgtgc ttccgatgtt catcatcatg acacttatgt acttcgtggt
    15841 atgttacgcg ctttctctgc tttccggtta cttggaaaag agacggtaca gttattaata
    15901 gatgattctt ttagcggttg tggcaaacta aagtcacaac cgcttttcct ctttccattc
    15961 gaaaaaaaga gatttcgatt gttttaagca agtaagagca cttgaaaatt cacgctaaaa
    16021 agtacacaat agggaggata gaatttattg aaaaaaagga tgatacgatt gacagataat
    16081 cgccctatcg gatttattga ttcaggtgtc ggcggcttga ctgtagtaaa agaagccctg
    16141 aaacaattac cgaatgaaaa tattttattt gtaggagaca cagcacgctg cccatatggc
    16201 cctagacccg cggaacaggt aatccagtat acttgggaaa tgacggatta tctggtggag
    16261 caaggaatca agatgctggt gatcgcctgc aataccgcaa ctgcggtggc tttagaagaa
    16321 atcaaagctg ctctttctat tccagtcatc ggtgtgatcc ttcccggtac tagagcggca
    16381 gtaaaaaaaa cacaaaataa acaagttggc attatcggta cgattggtac ggtaaaaagt
    16441 caagcttatg aaaaagcact gaaagagaaa gtaccagaat tgactgtgac aagtcttgct
    16501 tgtcccaaat ttgtttcagt tgtcgaaagt aatgaatacc attcatcggt ggcgaaaaaa
    16561 attgtggcag aaacattagc tcctttaacc actaaaaaaa tcgatacatt gattttggga
    16621 tgcacccatt atccattatt acgccccatc attcaaaatg taatgggaga aaatgttcaa
    16681 ctgatcgatt ctggagcaga aacagtaggt gaagtatcta tgctgttaga ttatttcaat
    16741 ttgagcaatt caccgcaaaa tggtcggaca ttatgccagt tttatacaac tggctctgcc
    16801 aaacttttcg aggaaatagc tgaagactgg cttggaatcg gacacttaaa tgtagaacat
    16861 atcgaattgg gaggaaaata aggatgcgtc atgacgggag aaaagcccaa gagcttcgca
    16921 aactggagct tcaaacaaat gttttcaaat atccagaagg atcggtagtg atccgttttg
    16981 gtgatacaac agttatttgt tctgcaacaa tagaaaattc tgttccccct tttttgagag
    17041 aaacaggaac aggatgggtg acagcagaat atagtatgct gcctcgagca acgagtacga
    17101 gaaatcgtag agaaagcagc aaagggaaac tttctggtag aacaatggaa atccagcgtc
    17161 ttattggtcg ttccttgcgt gcagtagtag acttggaaaa attaggagaa cgtagtatca
    17221 tcgtagactg cgatgtgatc caagcggatg gcggtactag aacagcaagt atcactgggg
    17281 catttgtggc attgaaatta gccattgaaa aacttttaag agaaaaagaa ttgtcagaag
    17341 atccaataaa agaacatctt gctgcagtta gcgtaggaat cttaccagat gggacttgcg
    17401 tcacagattt ggattaccaa gaagattccg cagcacttgt agacatgaat cttgtaatga
    17461 cagaatctgg aaaattcgtg gagattcaag gaactggaga agaagcaacg tttgatggtg
    17521 aacagttgaa tgaaatgctc ttttttggaa aaaacgcgat tgaagatttg atcaaagagc
    17581 aaaaacatgc tttgctaaca gagtttgctc aaaatgatga gcggatagaa gaaacaaaaa
    17641 caatcatcat tgcgacaaga aatccaggaa aagcagaaga gtttcgaaat atgttcaaag
    17701 aagcgggata tcatgtaaaa acattacttg actatcctga attgccagat gtggaagaaa
    17761 ctggcagcac atttgaagaa aatgcccgat taaaagcaga aacgattgca caattgcttg
    17821 atcagccagt cttagcagat gattcaggtt tgaaagtgga tgctttaggc ggtatgccag
    17881 gaatctattc tgcacgtttt gcaggggaac aaaaaagtga tgcggggaat aacgccaaat
    17941 tattatatga attgacagat gtgccggatg aaaaaagaac agcacaattc cattgtacgt
    18001 tagtattcgc ggcacccaaa aaagacagcc ttgtagtaga agcggaatgg cccggtcgag
    18061 tagcccgtat accatctggc gaaaatggct ttgggtatga tcctttattt atcccagaag
    18121 gtaagaaaca aacagcagca gaattatcga gcgaagaaaa aaacaagatc agtcatcggg
    18181 ctcaagcaat gaaaaaattg agtgccgagt ggaaacaatg gctggaagga gagagataga
    18241 tgcggtattt agtagtaagt gataatcatg gagacagaga tatcctcgtt gacttagtag
    18301 atcgttatcg ttctgaagtg gatatctttt tccattgtgg tgattcggaa ttagaagctt
    18361 ctgacccatt atgggataca ttccaagtgg ttcaaggtaa ttgtgattat ggaccaggat
    18421 tcgaacagaa aaaagtgatc caaactggtc aggatacagt attcatgact cacggtcatt
    18481 tatcgaatgt ccgatttggt ttgacacagt tagcaatcga agctcgagct gcaactgccg
    18541 atatggtgtt ttttggtcat acccatcaaa taggctgtga gatggatcag aaaatcctgt
    18601 ttttgaatcc tggtagcatt tctcagccta gaggcccgat ccaaatccca tcatatgctc
    18661 ttatcgaaag tttagatgac caaatttctg ttcaatacta taaccgtgcc catcaggcag
    18721 ttgaagagtt ggccttttct ttttcgagag agcgctgaat aaaaaaactc taatttttaa
    18781 taaaaataag ctttatttta tgaattttgc atccggtttc ggtaaaatag tagtgttgaa
    18841 tgaaatagaa atggaggcca tcagatgatt ggacctatag taagagaaat gctgctggaa
    18901 aatcaggaaa cgtttttgat tcctgcagaa aatgtggcaa atgttatgta tcatcatcca
    18961 ttgtcccatg gtttacttgt gttatcgaag gtcggttatt ccaaaattcc tgtattagga
    19021 aaagatgatc actttgtagg actagtaagt ttatcagatg tggtcaataa aatgattgac
    19081 ttgcaaacaa tcagcatgga cccgttagaa ggactaacgg ttgctgatgt gacagaaaca
    19141 gatgttccga caattgatga aaattgggaa ctagaagaag tactccattt gttagtcgat
    19201 tcgccttttt taccggttgt cacagaagat cggattttca aaggaatcat tacaagaaaa
    19261 gagctgctta aagccgtaaa cttcatggtg catgaattag aacgtcgtaa tatattgtct
    19321 ccgaaaatcg aagaattaaa agaacaaatc gatatcgtga gctaaagaac gagaaactaa
    19381 aagataatca aaaaataagt agaaaaaaca gcgtaccagt tttgacttta tcttgaaaaa
    19441 gaggctggga caaaagc
//
LOCUS       DACEEZ010000051        19130 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20313_2017
            SAMN12639966-rid9879223.denovo.051, whole genome shotgun sequence.
ACCESSION   DACEEZ010000051 DACEEZ010000000
VERSION     DACEEZ010000051.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639966
            Sequence Read Archive: SRR10023158
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 19130)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19130)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741683.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741683.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:44:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,016
            CDSs (total)                      :: 2,962
            Genes (coding)                    :: 2,889
            CDSs (with protein)               :: 2,889
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 36 of 73
            Pseudo Genes (incomplete)         :: 29 of 73
            Pseudo Genes (internal stop)      :: 18 of 73
            Pseudo Genes (multiple problems)  :: 8 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19130
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639966-rid9879223.denovo.051"
                     /isolate="E20313_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(<1..703)
                     /locus_tag="IWO89_001810"
     CDS             complement(<1..703)
                     /locus_tag="IWO89_001810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAQ0456543.1"
                     /translation="MFWHFYKYRTKILLRNKSMLFWTLAFPILLGLMFMAAFGEIDQG
                     GNLETINTGIVIKADQTALSENFTTVLEQVEVNDKPLFEVQELSEVEANEKLNADDLA
                     GFYLIDGSSVSLHVGQAGMSQTLMKNVMDQFLQRIGIISSKVAEVENVDLAPIMTVFE
                     QEVSFQEVHAGRNMSFKSFYFFTLIGMAILYGTMWGVRNVQDQQANQSSNGIRLSLIP
                     RKQSLVSIANLAASFT"
     gene            complement(717..1641)
                     /locus_tag="IWO89_001811"
                     /pseudo
     CDS             complement(717..1641)
                     /locus_tag="IWO89_001811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015509966.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
     gene            1758..2837
                     /locus_tag="IWO89_001812"
     CDS             1758..2837
                     /locus_tag="IWO89_001812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HAQ0456544.1"
                     /translation="MHKSRLLNETSFLLVISLLLLVSQGFVAMQVIYLLIAVLFTGVS
                     YLLPTKPNWIFQVILFLLCAFLPDLVLFLPATMRVIAPEESHLDYLFLLGFLLISTVG
                     NHPPIKVAFVVILLFFSFYLRKKDQRTTDLEKKYIVSKDTHWEKQQELSEQNKALALS
                     QETQLALQIVEERNRIARDIHDNVGHLLSSAILQIGALEMINQEPKLKEPLENLSATV
                     HTGMDRIRQSVHDLHETSISFQHSLEFLIADFPSSVTIEGNLFEGLNETQQNCFLMVI
                     KEALTNSIKHSHAKTVVLHFRTLPAFYRLQITNDGTSPQKNSAPGIGLKTMRQRVQEQ
                     KGQVHIFQENQQFQLTIILPREGHQ"
     gene            2869..3462
                     /locus_tag="IWO89_001813"
     CDS             2869..3462
                     /locus_tag="IWO89_001813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAQ0456545.1"
                     /translation="MTKSLQTILESTKEIRVLGIGHCAKDALVLYETHRPDVLLTDIR
                     MPKQTGIDAAKEILAKFPKAILLLLTTFKDEEYIREAFSIGVKGYLIKQNLQAIIPSV
                     KSAYNGQAVFGNEIIETFTQMMKNKPTIDSHAYASFSERELAIIKEVAAGKNNKEIAD
                     ALYLSDGTVRNYISQLLEKLDLRDRTQLAIYFYQHLQ"
     gene            complement(3572..3799)
                     /locus_tag="IWO89_001814"
     CDS             complement(3572..3799)
                     /locus_tag="IWO89_001814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005229738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HAQ0456546.1"
                     /translation="MAKETEDKLAKLAKESGNDFKKLDEMEKEFKLNESPDVERDDRE
                     ESKLEKTLDKAPIFPPNTPNNPTNPGSPANP"
     gene            complement(3902..6490)
                     /locus_tag="IWO89_001815"
     CDS             complement(3902..6490)
                     /locus_tag="IWO89_001815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RecD-like DNA helicase"
                     /protein_id="HAQ0456547.1"
                     /translation="MQPEEMPYFVGTVAAIFFQNPSNFYKVLLVHISETTADYREKEI
                     VVTGSFGDIQENETYRFYGELVDHPKYGRQLKLERYEQEKPTTANGIVAYLSSEKFPG
                     IGKKTAEKIVELLGEDAIEKIIADPSVLKQISGLTKPKREMLAETIRLNHGMDQVIMG
                     LNRYGFGSQLAFSIYQAYKNESLDIIEENPYQLVEDIEGIGFKRADNLAEQIGIAADS
                     PRRIRAAILHQIFQHAVQTGDTYVPAETLLQQTIRILETSRPIEIQPDQVATVIIQLV
                     EEGKIQQEETNLYENSLYFSEWGIGNSIQRLLSRKKEIHYPEKEVQKNIRRIEKIFGI
                     QYGPSQEQAIAEAIRSPLFILTGGPGTGKTTVINGIVNLFAELNGLELDPSKYTQEIF
                     PILLAAPTGRAAKRMNETTGLPASTIHRLLGLNGREKTPSVSTKELEGGLLIVDEMSM
                     VDTWLANTLFKAIPTNMQVILVGDKDQLPSVGPGQVLHDLLEIEQIPKMELNQIYRQG
                     DGSSIIPLAHEIKEGRLPKDFTKNQKDRSYFRSDAYQIEPLISKIVEKAKNKGFRAQD
                     IQVLAPMYRGPAGIDALNKMMQEIFNPGKPGKKEVHWNDTVYRIGDKVLQLVNTPELN
                     VFNGDMGEITGIILAKDSEDKVDELVIQFDANEVTYKRNEWNKITLSYCCSIHKSQGS
                     EFKMVILPMVHQYQRMLQRNLLYTAVTRSKELLILLGEEQAYVTCVANESASRMTTLK
                     SRIMDNDKMNITLRTKLAAYEDSLVGNDPFEDGNYTKEPRVVKEKLPVEDDSGTNETK
                     NQVMETVEPDLFSSFVGEEKKEEVEPAVSKDFVLTAEVIDNQLVDPMIGMENSSPYDF
                     IPAKNA"
     gene            complement(6510..7169)
                     /locus_tag="IWO89_001816"
     CDS             complement(6510..7169)
                     /locus_tag="IWO89_001816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HAQ0456548.1"
                     /translation="MELYFTRHGKTQWNQERRFQGSNGDSPLLPQSYEEIKAFGKKVK
                     YVPFKAIYSSTAKRARDTAEGINKELARPVEIFYTDKLRELGLGTLEGQSIDEMYQKY
                     PDNLPNLRNHLDLYDPSPFHGETIESAITRIETVVADAVAQHKDGPLLFVGHGASLTA
                     AIQWMTGKELSELREMGGLFNSSLTILETEEPCRLLPYELKVWNQTDFLGTETAPEPL
                     L"
     gene            complement(7218..7721)
                     /gene="trmL"
                     /locus_tag="IWO89_001817"
     CDS             complement(7218..7721)
                     /gene="trmL"
                     /locus_tag="IWO89_001817"
                     /EC_number="2.1.1.207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (uridine(34)/cytosine(34)/5-
                     carboxymethylaminomethyluridine(34)-2'-O)-
                     methyltransferase TrmL"
                     /protein_id="HAQ0456549.1"
                     /translation="MNHIVLFEPLIPANTGNIARTCAATNTPLHLIEPLGFSTDDKHL
                     KRAGLDYWNDVNITYHSNLEAFLTFLGNRKLHLISKFAHKVYSDENFADGEEHFFLFG
                     KETTGLPETFMRENEEKCLRIPMNDEHVRSLNLSNTAAMIVYEALRQQGFPNLELTHH
                     YENDKLD"
     gene            complement(7942..8808)
                     /locus_tag="IWO89_001818"
     CDS             complement(7942..8808)
                     /locus_tag="IWO89_001818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HAQ0456550.1"
                     /translation="MLKKIDRGRQFLAENHQLFRCPVCQEEVKKSDNGLICKNNHRFD
                     LSKKGTLYFLSHAVKTEYDQGMFVPRRRMIQSGMYQPVIETIAQHLPEEVSLLDVGCG
                     EGSFLNELSKLKKIQNAVGFDISKEGVYAATEQSAEAFWCVADLTNLPFADQTFNAVL
                     NIFSPSHYQEFQRVLSEDGEVLKVVPQENYLKELRQAFYPNQPEKQSYSNERVVAKFA
                     EAMKLTERQRVTYEFEIPEENQKDLLAMSPLEWQVEERIKADLYKNPLKKITIDIELL
                     KGTKRKRFSSSF"
     gene            8917..9558
                     /locus_tag="IWO89_001819"
     CDS             8917..9558
                     /locus_tag="IWO89_001819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper homeostasis protein CutC"
                     /protein_id="HAQ0456551.1"
                     /translation="MIKEFCAENYTSIPLAIARGADRIELCDNLAVGGTTPSTGVIEE
                     TLSYAGEKDIPVMTIIRPRGGDFVYNDIELKIMHTDLIEAKKLGTDGVVFGCLTPSGW
                     LDEEALELLIETAEGLQITFHMAFDSIPEDRQFEAIDWLVEHGVDRILTHGGPADKTI
                     VENFPRLKELIAYTDGRLIILPGGGVTAENAAHVAKELNVSEVHGTKIVDLQL"
     gene            complement(9620..10981)
                     /gene="mgtE"
                     /locus_tag="IWO89_001820"
     CDS             complement(9620..10981)
                     /gene="mgtE"
                     /locus_tag="IWO89_001820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter"
                     /protein_id="HAQ0456552.1"
                     /translation="MDENQELEERFAQLTQDLRENNIQAFRNSFLEMHIYEQGQFYQS
                     LTEENRQLIYSYLSPKELADMFDVIEEDNEHMKEYLDEMRPSYAADMLSEMYTDNAVD
                     LLNTLDKKQIAKYLSLMSADDASEIKELLHYEDETAGAIMTTEFVSIVANQTVRSAMY
                     VLKNEADVAETIYYIYVVNQEGQLVGVISLRDLIVNDDDAMISDLMSERVLSVHVGDD
                     QEDVAQTFRDYDFLALPVTDYDDHLLGIVTVDDIIDVIDDEAASDYSGLAGVNVEEIN
                     ENPVKAASRRLPWLITLLFLGMSTASLISHYEDLVSEASILAVFISLITGTAGNAGTQ
                     SLAVAVRRLAISDEKDHGFVRLVLAEVLTGVVTGAVTGITIFLIVGFWKHNFILGFVI
                     GMAMLFAITVANLAGSLIPMLMDRLGFDPAVASGPFITTLSDLTSVLIYFNIASLFMG
                     YFM"
     gene            complement(10999..11898)
                     /locus_tag="IWO89_001821"
     CDS             complement(10999..11898)
                     /locus_tag="IWO89_001821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HAQ0456553.1"
                     /translation="MQFTWDYEKDHSQQVKYFLKEKGISKGLLAKIKFQGGQIKVNDQ
                     VENVLFSLAKDDKVTIVIPAEGEHETVLLDETPIDIVYEDEHVLVVNKPAGVSSIPAQ
                     YHPNGTMANRVKAYYKKQGYENQVIHVVTRLDRDTSGLMLFAKHGFAHAKLDMQLREK
                     KFVKKYQALVSGRLSDLSAHGHIDLPIARDYSSLLKRMVSDEGRSAQTEYWIEKRLTE
                     IALVDIQLHTGRTHQIRVHFSAIGCALLGDDMYGGRMDLGIKRQALHCYDLRFYHPFT
                     EELLEFKQPLAADMEAVVRRFEE"
     gene            complement(11900..12697)
                     /locus_tag="IWO89_001822"
     CDS             complement(11900..12697)
                     /locus_tag="IWO89_001822"
                     /EC_number="2.7.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD kinase"
                     /protein_id="HAQ0456554.1"
                     /translation="MKVAIVHNKEAKTIEVTERLNALLEQAGIQRDDHEPELVISVGG
                     DGTLLSAFHHYSHCLNDVRFLGVHTGHLGFYTDWRDYELEELVDSLRTNREQSVSYPL
                     LDVRISYLDETPDQHFLALNESTIKRANRTMVADVYIKNELFESFRGDGLTISTPTGS
                     TAYNKSVGGAVIHPSINAFQLAEIASLNNRVFRTLGSPIVIAHDEWLEIKLENTEDYL
                     VTVDQLNVAKANIRSIYYRIAEERIHFASYRHMHFWHRVKDAFISED"
     gene            complement(12675..13379)
                     /locus_tag="IWO89_001823"
     CDS             complement(12675..13379)
                     /locus_tag="IWO89_001823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP pyrophosphokinase family protein"
                     /protein_id="HAQ0456555.1"
                     /translation="MERDWEAFLAPYEQTVSELKVKLRGIRKQFREQNRHVPIEFVTG
                     RVKPVDSIVTKSQLRHIPMGRLEEEMSDIAGLRIMCQFVEDIHQVVEIIRKRKDMKVL
                     QERDYITNKKASGYRSYHLVIEYPVQLISGEKKILAEIQIRTLAMNFWATIEHSLNYK
                     YQGVFPEEMSERLQRAAEAAYQLDEEMSSIREEIQEAQRLFSHGRGKSDDVYYRTLLK
                     KESEREEAGDESSDRS"
     gene            13510..14085
                     /locus_tag="IWO89_001824"
     CDS             13510..14085
                     /locus_tag="IWO89_001824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CYTH domain-containing protein"
                     /protein_id="HAQ0456556.1"
                     /translation="MTQMLEIEYKSMLTKQEYEIFIAHYQLTDKDFRVQTNIYFDTVD
                     EQLKKQNCGLRIRFVGHQAEYTLKTPAEKGRLETTDTFITEEAEAFIFEKRLPRSGAV
                     FQKLSDLNIDPASLIQTGKLTTKRAEFPIEEGLLAIDESWGDNLHDFELELEVGDASV
                     GKIAFINFLKRFSLPYRPAKNKIQRMLEAKN"
     gene            14203..14880
                     /locus_tag="IWO89_001825"
     CDS             14203..14880
                     /locus_tag="IWO89_001825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsbA family protein"
                     /protein_id="HAQ0456557.1"
                     /translation="MIEIYLFVNPLGEYCFEMEQQLLQFIEEEYGTPSKEKMQFRFLP
                     LVNLQTIGDVMQQKGISQNNLEERNHLFSMTYSAALDCKAAQFQGKKKGRRFLIKLQE
                     AVGCNDIPYSQELAESIFEEIGGDLNMFKEDRQSDFVKEVFFSDQKIAREMGITKHPS
                     AVVYNYACDRDFGVLVEVKGTSSLWRITELCKTTDNSYQVFNGEHLHTNDSQFHSRTS
                     HLHLLSN"
     gene            complement(14959..16767)
                     /gene="pepF"
                     /locus_tag="IWO89_001826"
     CDS             complement(14959..16767)
                     /gene="pepF"
                     /locus_tag="IWO89_001826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligoendopeptidase F"
                     /protein_id="HAQ0456558.1"
                     /translation="MEVKQLPKREELPENLTWDLTKIFSSDQEFDEKYLELSEELKQS
                     EKHKGTLDQGASQFLNAIEFVLRVYRQTEVIYVYAHLKNDQDTGNTDYQALYARASSL
                     FSKVSEAVSWFEPEILQLSDDQIWQYFKEEPKLEVYRHYIQQIVDNRAHVLSAEQESL
                     LAGAGEIFDASSDTFAVLNNADLVFPTIEGENGEIVQLSHGVYGQLLESTDRRVREAA
                     FKGLYSVYEQFRNTFASTLGTHIKGHNFKAKVRNYSSAREASLSNNHIPESVYDTLVD
                     VVNKHLPLLHRYMELRKRLLEVEKLHMYDLYTPVLGEAPITFTYEEAKEKALEALKPM
                     GEEYMAIVEKAFSERWIDVVENKGKRSGAYSSGSYDTNPYILLNWHDTLDQLFTLVHE
                     MGHSVHSYFTRSNQPYVYGDYSIFLAEIASTTNENILTEYLLETEKDPRVRAYVLNHY
                     LDGFKGTVFRQTQFAEFEHFMHTEDEKGVPLTSEYLSDSYGKLNAKYYGPAVEEDPEI
                     KFEWSRIPHFYYNYYVFQYSTGFSAASALAKKILNQEPEALENYLAYLKAGNSDYPVE
                     VMKKAGVDMTQAAYIEDAMSMFEQRLNELEELIDRL"
     gene            complement(16943..17941)
                     /locus_tag="IWO89_001827"
     CDS             complement(16943..17941)
                     /locus_tag="IWO89_001827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence protein CoiA"
                     /protein_id="HAQ0456559.1"
                     /translation="MLLAMNEYNKCCFAKHAEKGKKYYCRGCRKRVILKKGKKKCAHF
                     AHQKSDNCSVFSERESKEHLQLKECFMDWLGQSVEPVFLEAYLPRLRQRPDILLANLA
                     IEIQCSRLSHQRFIERTQNYLNNSYQVWWILGNSFLGQSQFSLIEKSCCYYNRKRGVH
                     CWKADLKKQKLYLYHHITETVSGHISFCSSCWTFSSRDLKEIFTSNEIKMNQMKKIEK
                     VSEDGKKWLTRQLIHKQKNTVSIQEQCYLRHKHLLHLSSWIYQKSRFFFYLQEQVFLY
                     RMLYEEALENQKVPDFNSWLCQIKEHKREWLFPMIDEEMVYQQFFNECIHLSSLKK"
     gene            complement(18167..18820)
                     /locus_tag="IWO89_001828"
     CDS             complement(18167..18820)
                     /locus_tag="IWO89_001828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adaptor protein MecA"
                     /protein_id="HAQ0456560.1"
                     /translation="MEMEHINENTIRVLIGHEDLEERGVSFLDLLGNHKEIENFFYSI
                     LEEVDIEEEFRGSEAVTFQVLPKGDGLELFISKNLPDEEISQSEDSTVDSEDVSDFLR
                     QQIIGSDPEEELSGSALEEQERQATFAFEDFEQVIQLATDVELESAWTDLYLFEERYY
                     LVVHFWMENLNQADVENQIARILEFAEKSDRTPETLNEYGQCLMARNAIERTKFYFV"
BASE COUNT         5525 a         4137 c         3324 g         6144 t
ORIGIN      
        1 acgtaaagct tgctgccaaa ttagcgattg aaactaacga ttgctttcta ggaatcaagg
       61 acaagcgaat accatttgac gattgatttg cttgctgatc ctgtacattg cggacccccc
      121 acatcgtccc gtagagaatc gccattccaa tcagagtgaa gaaataaaaa cttttgaaac
      181 tcatattacg acctgcatga acttcttgaa aagatacttc ttgttcaaaa acagtcatga
      241 tgggcgcgag atcaacgttc tccacttctg ccactttact cgaaattata ccaatacgct
      301 gtaaaaattg atccatgacg tttttcatca acgtctgact cattcctgct tgacccacat
      361 ggagcgaaac agatgaacca tcaatgagat aaaaaccagc caaatcatct gcatttagct
      421 tttcattcgc ttctacttcc gatagttcct gtacttcaaa taatggttta tcattgactt
      481 cgacttgttc taagactgtg gtaaaatttt ccgaaagcgc tgtttgatcc gctttgatta
      541 caattccagt attaatcgtt tcgagattgc caccttgatc aatctcgcca aaggccgcca
      601 taaacatcaa tcctaaaaga attggaaagg ccaatgtcca aaacaacatg gatttatttc
      661 gcagcagtat ttttgtgcga tatttataaa aatgccaaaa cattatttcc cctcctctag
      721 tctctcaaat ccttgccagt aatctctaaa aagacatcat tcaaggacgc ttctttggtt
      781 gctatttttc cgtaactgat cttttcttct tgtaagattg taagaatcgg caacaatact
      841 gtatttagtt cagtagttgt tacttggagc tgaccttttt tataagcgat tccatcagaa
      901 gctattgttc gctgacaaca cgttttctgg gagtaatggg atgtcgatcg tcaaacgatc
      961 cgccgtccgt accatatctt ttagagattc tttggttcct ttagcaatca cctgtccttg
     1021 atccatgatc acgatctggt cgcaaagaat ttctacttct tccatgtaat gtgttgtata
     1081 gacaatggtt gctccttgtc gattcagctc cttgatactt tctaaaattt tattccgact
     1141 ttgggggtct acagctactg tcggctcatc taaaaaaatt aaccgcggct tgtgggcaat
     1201 cccacatgcg atgttcaacc gacgtttcaa gccaccacta agttctcctg gacgaaacga
     1261 aacatatttt tctaatccca ctaattgaat cacctcttca atcaaggctt tccttttctc
     1321 tttttcggaa acatataaac cgcaaaaata atcgatattt tcttgcacac ttagttcgtc
     1381 aaagaccgca acgtcttgag gaacgacccc aatcattgcc ttgacagcat aattttcagg
     1441 agtcattgtt gtcccgaaca atttaatggt tcctttgtca taatttaaca aagaaagcat
     1501 acagttgatg gcagtcgatt tcccactgcc gtttggtcct aacagaccta agacttctcc
     1561 ttcttgtact tgcagatcaa aatgattcaa tgctacaaaa tctccatagc gtttgactaa
     1621 tttatcaatt tcgataatca tcatgaaaaa ctccttccaa atttcttgtt gatctgacta
     1681 taataaagtg caagaaaaga aactagtgac gattggctaa accacacatg acatttgtca
     1741 caatgaagga gtctgctatg cataaatctc ggctactcaa cgaaactagc tttctactgg
     1801 ttatctcatt gcttttgctg gtaagtcaag gattcgttgc tatgcaagtt atctatttat
     1861 tgatcgccgt tttattcact ggtgtcagtt atttactgcc tacaaagcca aattggattt
     1921 tccaagttat tctatttctt ttatgtgcct ttttacctga tcttgtcctg tttctgccag
     1981 cgacgatgcg agtgatcgcc cctgaagaaa gccaccttga ttacttgttt ttgttaggtt
     2041 ttttgttgat ttcgacggtt ggtaatcatc cccccatcaa ggtcgccttt gtcgtaattt
     2101 tgctgttttt ttccttttac ttacgaaaaa aagatcaacg aacgaccgat cttgagaaaa
     2161 agtacattgt ctccaaagat actcattggg aaaaacaaca agagttatcg gaacaaaata
     2221 aagcattggc cctgtcacaa gaaacacaat tagccttgca aattgtggaa gaacggaacc
     2281 gaattgcccg agacatccat gacaatgtcg gtcatctgct gtcaagtgcg attttacaaa
     2341 ttggtgcgct tgaaatgatc aatcaagaac caaaactaaa agaaccatta gaaaatttaa
     2401 gtgccaccgt tcacactggg atggatcgga ttcgtcaaag tgtccatgac ctccatgaga
     2461 catcgatctc ttttcaacac tccttagagt ttctgatcgc tgattttcct tcttctgtta
     2521 cgattgaagg aaacttgttt gaaggattga atgaaacgca gcaaaactgc ttcttaatgg
     2581 tcatcaaaga agccttaacc aacagtatca aacatagtca tgccaaaacc gttgttttac
     2641 attttcgaac gttgccggcc ttttatcgct tgcaaatcac gaatgatggc accagcccac
     2701 aaaaaaattc tgctcccggt attggtttga aaacgatgcg tcaacgtgtt caagaacaaa
     2761 agggacaagt ccatattttc caagaaaacc agcaatttca gttaacgatc attttaccaa
     2821 gggagggaca ccaatgataa cagtagtcat tattgagatg atccatttgt gacgaaatcg
     2881 ttacaaacca ttttagaaag cacgaaagag atccgtgtgt taggcattgg tcattgtgcc
     2941 aaggatgcgc tagtcttata cgaaacccat cgtcccgatg ttttgctgac ggacatccgc
     3001 atgcctaaac aaacgggcat cgatgctgcc aaagaaattt tggctaaatt tcccaaagcc
     3061 atccttttgc ttttgacgac attcaaagat gaagaataca tccgcgaagc attttccatc
     3121 ggtgtcaaag ggtatttaat caaacagaac ttgcaagcga ttattccttc tgtcaaatcc
     3181 gcttataatg gacaggccgt atttggtaat gaaatcatcg agactttcac tcaaatgatg
     3241 aagaataaac cgacgattga tagtcatgct tatgcttctt ttagtgaacg tgagcttgca
     3301 atcatcaaag aagtagctgc cgggaaaaac aataaagaaa ttgcagatgc cttgtacttg
     3361 agtgacggca ctgttcgcaa ttacatcagc caattactag aaaagctaga tttacgggat
     3421 cgcacgcaat tggctatcta tttttatcag catttgcaat aagtcttatc taacacaaaa
     3481 aaacctgaat gatgctcctt cgcatcattc aggttttttt cacatcttct attcaattgt
     3541 cccgcaaacc atttcctcac gattgaaaga atcaaggatt cgcagggctg ccaggattcg
     3601 ttggattgtt cggcgtgtta ggcggaaaaa taggcgcctt gtctaacgtt ttttctagtt
     3661 ttgattcttc acgatcgtct cgttctacat caggactttc attcaatttg aattcttttt
     3721 ccatctcatc taacttttta aagtcattgc cagattcttt tgctaatttc gctaatttat
     3781 cttcggtttc tttagccata aagtaacgcc ccccttttga tttcagctta gcatatcaca
     3841 ctgaaaaaac aaaagaaaac gctaaccaac gatcattttt agctatttct catctttaaa
     3901 atcaagcatt ttttgccggt ataaaatcgt agggagaact gttttccata ccaatcatcg
     3961 gatctactaa ctggttatcg atcacttctg ctgtaagaac gaaatcttta gaaacagcag
     4021 gttctacctc ttctttcttt tcttctccaa caaatgaaga aaacagatct ggttccactg
     4081 tttccattac ttggtttttc gtttcattcg tcccactgtc atcttctaca ggtagctttt
     4141 cttttacaac ccgtggctct tttgtatagt tcccatcttc aaatggatca tttccgacca
     4201 aagaatcttc ataagctgct agtttggtcc ggagagtgat attcatttta tcattatcca
     4261 tgatccttga tttcaacgta gtcatccggc ttgctgattc attcgctaca caagtgacat
     4321 aggcttgctc ttcccctaac aaaatcaaca gttccttact gcgagtcact gccgtgtaga
     4381 gcaaattacg ctgaagcatc cgctgatatt gatggaccat gggcaaaata accatcttga
     4441 attcactgcc ttgtgattta tggattgagc aacaataaga aagagtgatt ttattccatt
     4501 cgtttctttt ataagtgacc tcatttgcat caaactggat aactaattca tccactttat
     4561 cttcagaatc ttttgccaag atgatacccg tgatttctcc catatctcca ttgaatacat
     4621 tcaattcggg agtattgact aattggagga ctttgtctcc tatccgataa acggtgtcgt
     4681 tccaatgtac ttcctttttt ccaggtttcc ctggattgaa tatctcctgc atcattttat
     4741 ttaacgcatc gatccctgca ggtccccgat acattggtgc taatacttgg atatcttgtg
     4801 cacggaatcc cttatttttc gctttttcca caattttaga gatcaagggt tcaatttgat
     4861 aagcatcact tcggaaatag gaacgatctt tttgattctt tgtgaaatct tttggcagac
     4921 gtccttcttt gatctcatgg gcaagaggaa taatactaga accatcacct tgccgataaa
     4981 tctgattgag ttccattttg ggaatttgtt cgatttccaa aagatcatgg agtacttgac
     5041 caggaccgac agagggaagc tgatctttgt ctcctactaa gataacttgc atattcgtcg
     5101 gaatcgcttt gaataatgtg ttagccagcc atgtatcgac catagacatc tcatcaacaa
     5161 tcaaaagtcc gccttctaat tctttcgtac tgactgaagg tgttttttcc cgtccattca
     5221 gccccagaag acgatggatc gtactggcag gcagacctgt cgtttcgttc attcgtttag
     5281 ctgctcggcc agttggtgct gcaagtagaa taggaaaaat ttcttgggta tattttgaag
     5341 gatccaattc cagtccgttc aactctgcaa aaagattgac gattccgttg atcacagtag
     5401 ttttccccgt acctggtcct cctgttaaaa taaatagagg ggaacggatt gcttcagcga
     5461 tagcttgttc ttgcgaaggt ccatactgta tgccgaatat tttttcgata cgccgaatat
     5521 tcttctgcac ttctttttca ggataatgga tttctttttt acgactgaga aggcgctgga
     5581 tcgaattacc gatcccccat tcagaaaagt acaaactgtt ttcgtaaaga ttagtctctt
     5641 cttgttgtat ctttccttct tcaacaagtt ggataattac agtagcaacc tgatcaggct
     5701 ggatttcaat tgggcgactt gtttctaaaa tacggatcgt ttgctgaagc aatgtctccg
     5761 ccggtacgta tgtatctcct gtttgaactg catgctgaaa aatctgatgc aagatagctg
     5821 ctcgaattct tctcggtgaa tctgctgcaa taccgatttg ttcggctaga ttatctgcac
     5881 gtttgaaccc gattccttct atgtcttcga ctagctggta cggattttct tcgatgatat
     5941 ccaaagactc gttcttatag gcctggtaga tactgaaagc taactgacta ccgaacccgt
     6001 aacgattcag tcccatgatc acttgatcca tcccgtggtt tagccgaatc gtctcagcaa
     6061 gcatttctct ttttggcttc gtcaaaccgg agatttgttt taataccgaa ggatcagcaa
     6121 tgattttttc gatagcatct tctcccagca attctacaat tttttctgct gttttcttcc
     6181 cgattcctgg gaatttttcg ctggaaagat acgctacgat cccatttgca gttgttggct
     6241 tttcttgctc atatctttcg agtttaagct gccttccgta tttaggatga tccactaatt
     6301 ctccataaaa acgataagtc tcattttctt gaatatcgcc gaaacttccg gtgactacaa
     6361 tctctttttc acggtaatca gctgtggttt cactgatgtg taccaaaagc actttgtaaa
     6421 aattactagg attttgaaaa aaaatcgcgg caactgtacc aacgaaataa ggcatttctt
     6481 ctggctgcat cggaagccgc ctccttcttc tatagtaaag gttctggtgc tgtttcagtt
     6541 cctaaaaaat ctgtttggtt ccatactttc agttcatatg gcaataatct gcaaggctct
     6601 tcagtctcta aaatcgtcaa actactattg aacaatccac ccatttcacg caactcactt
     6661 agctcttttc ctgtcatcca ttgaatggct gcagttaaag aagctccatg tcctacgaat
     6721 aaaagtgggc catctttatg ttgggcaacc gcatctgcta caacagtttc gatacgtgtg
     6781 attgccgatt caatcgtttc tccgtgaaaa ggagaaggat catacagatc taaatgatta
     6841 cgcaaattcg gcagattatc cggatatttc tggtacattt catcgatcga ctgaccttct
     6901 aaagtaccca gccctagttc tcttagctta tctgtataaa aaatctctac cggacgagca
     6961 agctctttgt tgataccttc tgctgtatca cgtgctcgct ttgctgtact ggaataaatt
     7021 gctttaaaag gaacatattt tacctttttc ccaaatgctt tgatttcttc gtaactttgt
     7081 ggcaaaagcg gagaatcgcc attgctccct tgaaaacgtc tttcctgatt ccattgtgtc
     7141 ttcccatgtc gggtaaaata tagttccata cgcagcaaac tcctgtcttt cttttatttt
     7201 actctataat aaactgtcta atccagcttg tcattttcat agtgatgcgt caattctaaa
     7261 ttagggaatc cttgctggcg aagtgcttca tacactatca ttgcagctgt attcgataaa
     7321 ttcaatgagc ggacatgctc atcattcata gggatgcgta aacacttttc ttcgttttca
     7381 cgcataaatg tttctggcaa accggttgtt tcttttccaa ataaaaagaa gtgctcttct
     7441 ccatcagcaa aattttcgtc actatatact ttatgggcaa attttgagat caaatgtagt
     7501 ttacgattac ctaaaaatgt taaaaaagct tctaaattac tgtggtaagt aatatttaca
     7561 tcattccaat aatccaaccc tgcccgtttt agatgtttgt catcagttga aaaacctagt
     7621 ggctcgatta aatgaagcgg agtattcgtg gctgcacacg tacgggcaat atttccagtg
     7681 tttgcaggga ttaatggttc aaataaaacg atatgattca tcaggatcat tcctctcata
     7741 tttatgttcg tttctatttt ctgacaattt gatttttgca taaaaaaaaa cgtatcaact
     7801 cggtgtcagt cactgcgagt cgttacgtta gtgaagctct tctcactaac ttttatcaga
     7861 acaggaacct gataaaaacc aatgaaaaaa caacttgttg ctaatataac accctcaact
     7921 tttttttgca acctctactc attaaaaact gctagaaaaa cgttttctct tagttccttt
     7981 taataattct atgtcaattg taatcttctt caaaggattc ttatataagt ctgctttgat
     8041 tcgctcttct acctgccatt ctaaaggtga catggccaaa agatcttttt gattttcctc
     8101 tggtatctca aattcatagg ttacacgctg acgttcagtt aatttcattg cttcagcaaa
     8161 cttggctact acacgttcat tggaataact ctgtttttca ggttgattcg gataaaaagc
     8221 ctgacgtagt tcctttaaat aattttcttg tggcaccacc ttcaacactt ctccatcttc
     8281 tgagagtaca cgttggaatt cttggtaatg agaaggcgaa aagatattta aaaccgcgtt
     8341 gaatgtttga tcagcaaacg gcaaattggt caaatcagct acacaccaaa atgcttcggc
     8401 agattgttct gtagctgcgt agactccttc ttttgaaata tcaaatccaa cagcattttg
     8461 aatctttttc aattttgaaa gttcattcaa gaagctacct tctccgcagc caacatctaa
     8521 tagcgagact tcttcaggta aatgctgcgc aatagtctca ataacgggct gatacatgcc
     8581 agattggatc attctacgcc ttggaacgaa cattccttga tcgtattctg ttttaaccgc
     8641 atgggataaa aaataaagtg ttcctttttt ggaaaggtca aaacggtgat tatttttgca
     8701 tatcaagcca ttatcagact tttttacttc ttcttggcaa accggacagc ggaacagctg
     8761 atgattttct gccaaaaact ggcgtcctcg gtcaattttt tttaacatag ttccccctca
     8821 tttctgtttt atcttaccgt tagatgctca ctttggcaac tgctaaaaat atgatacaat
     8881 gcctagaaga aagtgaaaga taaaggagaa gattagatga tcaaagaatt ttgcgctgaa
     8941 aactacacat ctattcctct agcaattgct agaggagccg atcggatcga attgtgtgac
     9001 aacttagcgg tcggtggcac aaccccgagt actggtgtga tcgaagaaac gttaagttat
     9061 gctggggaaa aagatatccc cgtaatgacg attattcgtc ctagaggggg cgacttcgtc
     9121 tataacgata tcgaattgaa aatcatgcat actgatttga ttgaagcaaa gaaattaggg
     9181 acggatggcg tagtatttgg ttgtcttact ccttcaggct ggctagacga agaggcctta
     9241 gaactattga tcgaaacagc agaagggttg cagatcacct tccatatggc ttttgacagt
     9301 attcctgaag atcggcaatt cgaagcaatc gattggctgg tagaacatgg tgttgaccgt
     9361 atcttgactc atggcggtcc tgctgacaaa actattgtag aaaatttccc acggcttaaa
     9421 gaattgattg cctatacaga tggccgctta atcattttgc ctggtggtgg tgtcactgca
     9481 gagaatgcag cacatgtagc taaagaattg aacgtaagcg aagtacacgg aaccaaaatc
     9541 gtggatcttc agttataatc ttataaacaa aaaaacattt gcaacaggca taatgcttat
     9601 tgcaaatgtt tttgtttttt tacataaaat agcccataaa taaagaagca atattgaaat
     9661 aaatcagtac actcgtcaaa tcgctcaaag tcgtgataaa gggaccgctt gctactgctg
     9721 gatcaaatcc tagacgatcc attagcatcg ggattaaact acctgcaaga ttcgccactg
     9781 tgattgcaaa tagcatagcc atccctatga caaaccccaa gataaaatta tgtttccaaa
     9841 aacctacgat tagaaaaatc gtgatccccg taacagctcc tgtgacaacg ccggtcaaga
     9901 cttcagccaa aacaagtcga acaaatccat gatctttctc atcagaaatc gcaagtcgac
     9961 gaacagccac agctagcgac tgggttccag cattaccggc agttcctgta atcagcgaaa
    10021 tgaagactgc taaaatactc gcttcactta ccaagtcttc atagtgactg attaaagaag
    10081 cggtcgacat acccaaaaac aacagagtga tcagccaagg caaacgtcga gacgctgcct
    10141 ttactggatt ttcattaatt tcttccacat tgaccccggc aagtcctgaa taatcgctag
    10201 cagcttcatc atcaataacg tcgatgatat catcgactgt gacgattcct aacaaatgat
    10261 catcataatc ggtaacagga agagctaaga agtcataatc tctaaatgtt tgtgccacat
    10321 cttcttggtc gtcgcctaca tgaactgaaa gcactcgttc gctcatcaga tcagaaatca
    10381 ttgcgtcgtc atcattcacg attaggtcgc gcagagaaat aacccctact agctgacctt
    10441 cttgattaac gacatagatg taatagattg tttcagctac atctgcttcg ttcttcagca
    10501 cgtacatcgc agagcgaaca gtttgattgg ctacgatcga aacaaattca gttgtcatga
    10561 tcgcgccagc cgtttcatcc tcgtaatgga gaagttcctt gatttcactg gcatcgtctg
    10621 ccgacatcaa acttaaatat ttagcaattt gctttttatc cagcgtatta agcaagtcaa
    10681 cagcattatc tgtatacatc tcagaaagca tatcagctgc atagctagga cgcatttcat
    10741 ctaggtattc tttcatatgt tcgttgtctt cttcaatcac atcgaacata tccgctaatt
    10801 ctttaggcga cagataggaa tagatcagct gcctgttctc ttctgtaaga gattggtaga
    10861 attgcccctg ctcatagata tgcatctcta aaaaactgtt tctgaatgcc tgaatattat
    10921 tttcccttaa atcttgagtc aactgggcaa atctttcctc taattcttga ttttcatcca
    10981 aaccaatctc acctcgcctt attcttcaaa ccgtctcaca actgcctcca tgtctgcggc
    11041 taaaggctgc ttgaactcca gcaactcttc tgtaaatgga tggtaaaaac gcagatcata
    11101 acaatgaagg gcttgtcttt ttattcccag atccattcgc ccgccataca tatcatcacc
    11161 gagcagggca cagccaattg cggaaaaatg aactcttatt tgatgtgtcc ttcctgtatg
    11221 cagctggata tcaactaacg cgatttctgt tagtcgcttc tctatccagt actcagtctg
    11281 tgccgaacgt ccttcatcag agaccattcg cttcaatagc gaagaataat ctcgggcgat
    11341 cggcaaatcg atatgtccat gtgccgataa atctgaaagc cttccactga ctaaggcctg
    11401 atattttttc acaaacttct tttcacgaag ttgcatatct aattttgcgt gcgcaaatcc
    11461 atgctttgca aaaagcatca aaccagatgt atcccgatct agtcgcgtca ccacatggat
    11521 tacttggttt tcgtaacctt gttttttata ataagccttt acacgattag ccattgtccc
    11581 atttggatgg tattgggcag gaatagacga aacgcctgct ggtttattta ctaccagaac
    11641 gtgttcatct tcatacacga tatcgatcgg tgtttcatcc aaaagtacgg tttcatgttc
    11701 tccttcagca ggaatcacaa tggttacctt atcatcctta gctagagaaa acaagacatt
    11761 ttctacttga tcattaactt tgatttgacc gccttgaaat tttattttcg ctaacagacc
    11821 ctttgagatc cctttttctt ttaaaaaata ttttacttgc tgagaatgat ctttctcata
    11881 atcccatgta aactgcatat caatcctcac taataaaggc atctttcaca cgatgccaaa
    11941 aatgcatatg tcgataagaa gcaaaatgga tccgttcttc tgctattcga taataaattg
    12001 agcggatatt tgcttttgcg acattcaatt gatcaaccgt tacaagataa tcttctgtat
    12061 tttccaattt tatttctagc cattcatcat gggcaatcac gatcggagaa cccaaagtac
    12121 gaaacactcg attattcagt gaagcaatct ctgccaactg aaaggcattg atactaggat
    12181 ggatcacagc accgccgaca cttttattat atgctgtcga accagtaggc gtagaaatcg
    12241 tcaatccatc tcctctaaaa ctttcaaaca gctcgttttt gatgtataca tcggctacca
    12301 tcgtacgatt ggcccgttta atcgttgatt cattcagtgc taaaaaatgc tggtccggtg
    12361 tttcatccaa ataactgatc cgaacatcca gcaagggata actaacactt tgttcccggt
    12421 tcgttcgtaa gctgtctact agttcttcca gctcgtaatc ccgccaatcc gtgtaaaacc
    12481 ctaaatgacc tgtatgcacg cccaagaaac gcacatcatt cagacaatga ctgtagtgat
    12541 ggaacgccga caataatgtg ccgtctccgc cgactgagat gaccaattct ggctcatgat
    12601 catctcgttg aatccctgct tgttctaaaa gtgcgttcaa acgttctgtc acttctatag
    12661 tctttgcttc tttattatga acgatcgcta ctttcatccc ctgcctcctc tctttcgctt
    12721 tctttcttca aaagcgttcg atagtagaca tcatctgatt ttcctcttcc atgggagaat
    12781 aaccgctgtg cttcttggat ttcttcacgg atactggaca tctcttcatc caactgatag
    12841 gcagcttctg ccgcacgctg aagtcgttca ctcatttctt ctggaaaaac gccttgatat
    12901 ttataattca gcgaatgctc aatcgttgcc cagaaattca tcgcaagagt ccgaatctgg
    12961 atttccgcca aaattttctt ctcaccgctg atcagctgta caggatactc gatcaccaga
    13021 tgataagaac ggtagccact tgctttttta tttgtgatgt aatctcgttc ttggagaact
    13081 ttcatgtctt ttcttttacg gatgatctca acaacttgat ggatatcttc tacaaactga
    13141 cacatgatcc ttaacccggc aatatcagac atttcttctt ctaaacgtcc cattggaata
    13201 tgccgtaatt gtgattttgt tacaatacta tcgactggct tgactctccc agtgacaaat
    13261 tcaatcggga catgtcgatt ttgttcacgg aactgtttgc ggatcccccg cagtttcact
    13321 ttcaattctg atacagtttg ttcatatggt gctaaaaatg cttcccaatc tcgttccata
    13381 gctgatacct ctacttcatt aacattcatt gtttattcta ccatatatgc aaagtttttt
    13441 cgtactcttg atttatgcat tgaccaaaaa aagaaaatta ggcaaaatag acaaaaagga
    13501 gtcgatcaaa tgacacaaat gcttgaaatc gaatataaat ctatgctgac aaagcaagag
    13561 tacgagatat tcatcgctca ctatcaatta acagataaag atttccgtgt acagactaat
    13621 atttatttcg ataccgttga tgaacagctg aagaaacaaa attgtggcct acggatccgt
    13681 ttcgttggtc atcaagctga atatacatta aaaacacctg ctgaaaaagg gcgtctcgaa
    13741 acaacggata cctttataac agaagaagca gaagcattta tcttcgaaaa acgtctgcct
    13801 cgttcaggcg cagtttttca aaaactttct gatttgaata tcgatcctgc ttcattaatc
    13861 caaaccggaa agcttacaac aaaacgggct gaattcccta ttgaagaagg acttttagct
    13921 attgatgaaa gctggggaga caatctacac gattttgaac tggaactaga ggtaggggat
    13981 gcatctgtgg gaaaaattgc ttttataaat tttttaaaac gtttttcttt accttatcgt
    14041 cctgctaaga acaagatcca acgtatgctt gaagctaaaa attagaaacc tttcattttc
    14101 acaaggcact tgagttcatt tttttacttt tttctgttaa gttataggat gtgaaagaat
    14161 ctagtcacta gttgtataga atctattttg ggggagattc atatgatcga aatctattta
    14221 tttgttaatc ctttagggga atactgtttc gaaatggaac aacagttatt acaatttatc
    14281 gaagaagagt acgggacacc atcaaaagaa aagatgcaat ttcgctttct tcctttggtc
    14341 aatttgcaga caattgggga tgtcatgcaa caaaaaggaa tttctcagaa caatcttgag
    14401 gaacgtaatc acttattctc aatgacttat tctgcagcat tggactgcaa agcggcgcag
    14461 tttcaaggaa agaaaaaagg ccgccgtttt ttgatcaaat tgcaagaagc agtcggctgt
    14521 aatgacatac catattctca agaacttgct gaatccatat ttgaagaaat cggcggcgat
    14581 ttgaatatgt ttaaagaaga tcgtcagtct gacttcgtta aagaagtatt cttttctgat
    14641 caaaaaattg ctcgggaaat gggcatcaca aaacaccctt ctgcagtcgt ttacaattat
    14701 gcctgcgacc gtgattttgg tgttttagta gaagtaaaag gtacaagtag cctttggaga
    14761 atcacagagc tgtgtaaaac aacagataac tcatatcaag tttttaatgg tgaacattta
    14821 catacaaatg attctcagtt ccattcaaga actagccatt tacacttgct ttctaattaa
    14881 aaaaatacgt cgactccctt acaagagagc cgacgtattt ttttcgtttc tattcgagaa
    14941 aatcaaaaat agaaagcctt ataaacgatc gatcaattct tctaattcat ttagacgttg
    15001 ttcaaacatc gacattgcat cttcaatata tgcagcctgt gtcatatcaa ctcctgcttt
    15061 tttcattact tccacaggat aatcgctgtt acctgctttt aggtaagcca agtagttctc
    15121 tagtgcctct ggttcctggt ttagtatttt cttcgccagt gctgaagcag cagaaaagcc
    15181 agtcgagtat tggaaaacat agtaattata ataaaaatgc ggtatccgtg accattcgaa
    15241 tttaatttct gggtcttctt cgactgctgg accataatat tttgcattca atttaccata
    15301 actatcactt aggtattcac tagtcaatgg cacacctttt tcatcttccg tatgcatgaa
    15361 atgctcgaat tcagcgaatt gtgtctggcg gaaaactgtt cctttaaacc catccaagta
    15421 atggttgagt acataagccc gtacacgagg gtctttttct gtttccaata aatactccgt
    15481 taggatattt tcattcgttg tcgaagcaat ttctgctaaa aagatggagt agtcgccgta
    15541 cacataaggc tggttcgaac gagtgaaata actatgaaca ctatgtccca tttcgtggac
    15601 aagcgtaaat agctgatcca gcgtatcatg ccaattcaat aaaatatatg gatttgtgtc
    15661 atagcttccc gaagaataag caccgcttcg tttcccttta ttttcgacaa catcgatcca
    15721 acgttcagag aatgcttttt ctacgatggc catgtattct tcacccattg gtttcagtgc
    15781 ttctaaagct ttttcttttg cttcttcgta cgtaaaggta attggagctt cccctaagac
    15841 cggtgtataa agatcataca tgtgcagttt ttccacttct aataaacgtt tccgtaattc
    15901 catgtatcga tgtaacaaag gcaaatgctt gtttaccacg tctaccaaag tatcgtatac
    15961 actttcagga atatgattat tgctcaaaga cgcttctctg gcagagctgt aattacggac
    16021 tttcgcttta aaattatgtc cttttatatg tgtgcctaaa gtagaagcaa atgtatttct
    16081 aaattgttcg taaacactgt acaatccctt aaatgctgct tcgcgcaccc ttcgatccgt
    16141 gctttctagc aactgaccat acacgccatg agataattgg actatttcac cattttctcc
    16201 ttcaatcgtt ggaaaaacta gatctgcatt attcaaaaca gcaaatgtat cacttgaagc
    16261 atcaaagatt tcacctgctc cagcaagaag agattcctgc tcagcagata agacatgggc
    16321 tcgattatct actatttgct ggatataatg gcgatagact tccaattttg gttcttcttt
    16381 gaaatattgc caaatctggt catctgacaa ttgcaatatt tctggttcaa accaggaaac
    16441 ggcttcacta actttcgaaa acagactgct tgctcttgca taaagcgctt ggtaatctgt
    16501 atttccagtg tcttgatcgt ttttaaggtg cgcatataca taaatgactt cagtttggcg
    16561 ataaaccctc aatacgaatt caatcgcatt taaaaattga gaagcgcctt gatcaagtgt
    16621 tcctttgtgt ttttcagatt gttttaactc ttctgataat tccaaatatt tctcatcaaa
    16681 ctcttggtcg cttgaaaaga tcttggtcaa gtcccaagtt aaattttcag gcaattcttc
    16741 tcgttttggc aactgcttta cttccatgaa atccactcct tttcttccct tagctctatc
    16801 tatcaaaata gatcatagtc taacatccac atgtcacagc atctaacttt tctactcatt
    16861 catgtgtctg cttccatcaa ttttagcata actaaatctt gttttacata gaaaaccgat
    16921 tcctagccta tttttatact tactattttt tcaaagaaga caagtgtatg cattcgttaa
    16981 aaaactgctg atagaccatc tcttcatcaa tcataggaaa aagccattcc cttttatgtt
    17041 cttttatttg gcaaagccat gagttgaaat caggaacttt ctggttttcc aaggcttctt
    17101 cgtacaacat tcgataaaga aaaacctgtt cttgtaaata aaagaaaaac cgactttttt
    17161 gatagatcca cgatgataga tgcaaaagat gtttatgacg caagtagcat tgttcttgta
    17221 tggatactgt attcttttgc ttgtgaataa gttggcgtgt cagccatttc ttcccatctt
    17281 cactcacttt ttctattttt ttcatttggt tcattttaat ttcgttacta gtgaatattt
    17341 ctttaaggtc cctactagaa aaagtccagc agctactgca aaaggaaata tgccctgata
    17401 cagtctctgt aatatgatga tacaaataaa gcttttgttt cttgagatca gctttccagc
    17461 aatgcactcc tctttttcta ttatagtagc agcaactttt ttctatcaat gagaattgcg
    17521 attgtccaag aaaagaatta ccaagtatcc accatacttg gtaactgtta ttcaaataat
    17581 tttgggttct ttcaataaat ctctggtgag acagtctaga acattggatt tctattgcta
    17641 gattcgccaa caaaatatcc ggcctttgcc gcaacctcgg caaataggct tccaaaaata
    17701 ctggttctac tgactgtccc agccaatcca tgaagcattc ctttaactgt aagtgctctt
    17761 ttgattctct ttcactaaag accgaacagt tatctgattt ttgatgggca aaatgagcac
    17821 attttttctt tccctttttc aaaatcactc tttttcgaca gccacgacaa taatatttct
    17881 ttcctttttc tgcatgttta gcaaaacagc acttattata ttcattcatt gcaagcagca
    17941 tttttctccc tctgaactta tttacgtcta agaatttatt tacacgaaaa aagagggcgt
    18001 gaaaaagcta ttctgcatca agtaataaga acagctaaac aaaaatagtt cccaatattt
    18061 ttcgtttggt tgggcttgtt accgcagatg caagcttttg aacacattta tacgaaaaaa
    18121 agactgcgac acaatttttg tcacagtctc ttactataat gctgatttat acaaaataaa
    18181 atttcgttcg ctcgatagca ttacgcgcca tcagacattg tccatattca ttcaatgttt
    18241 ctggtgtacg atcgctcttt tcagcaaact ccaaaattcg agcaatctga ttctctacat
    18301 ccgcttggtt gagattttcc atccagaagt gaacgactag gtaatagcgc tcttcgaata
    18361 aatacaaatc agtccaagca gactctagtt cgacatctgt tgcaagttgg atcacttgtt
    18421 caaaatcttc aaaagcaaaa gttgcttgtc tttcctgttc ttctagggcg cttccagata
    18481 attcttcctc cggatcgctg ccaataatct gctgacgaag aaagtcactc acatcttctg
    18541 aatcaacagt agaatcttct gattgagaaa tttcttcatc cggaagattt ttactgataa
    18601 acagttctaa tccgtctcct tttggcaaaa cttggaaagt gacagcttcg cttcctcgga
    18661 actcctcttc gatatcaact tcttccaaaa tactataaaa gaaattttcg atttctttgt
    18721 gattgccaag taaatccaaa aatgaaacgc cccgttcctc taaatcttca tgaccaatta
    18781 gtacacggat cgtattttca ttaatatgtt ccatttccat tttgctacac ctcactttgc
    18841 cacaatgttt aatatagatt cattgtagcg aaatcagctt aaatgtaaag aaaaaaaatc
    18901 tcgctgttaa gcgaatttaa taaaaccagc ggatttttca acctttcaag ctatcttatt
    18961 taactataac atttttggca gacgaatact atatagccga cttttcagct aattttccct
    19021 ctctattttt cgtgcaatca tcatcttcta ctaccttttt atacatctaa tcaagaatac
    19081 tttttgtaaa aacaaaaaaa cttggaaaaa ctatctttgt aatagtcttt
//
LOCUS       DACEEZ010000052        18739 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20313_2017
            SAMN12639966-rid9879223.denovo.052, whole genome shotgun sequence.
ACCESSION   DACEEZ010000052 DACEEZ010000000
VERSION     DACEEZ010000052.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639966
            Sequence Read Archive: SRR10023158
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 18739)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18739)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741683.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741683.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:44:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,016
            CDSs (total)                      :: 2,962
            Genes (coding)                    :: 2,889
            CDSs (with protein)               :: 2,889
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 36 of 73
            Pseudo Genes (incomplete)         :: 29 of 73
            Pseudo Genes (internal stop)      :: 18 of 73
            Pseudo Genes (multiple problems)  :: 8 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18739
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639966-rid9879223.denovo.052"
                     /isolate="E20313_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            255..530
                     /locus_tag="IWO89_001829"
     CDS             255..530
                     /locus_tag="IWO89_001829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0456561.1"
                     /translation="MRESFEQQKKLLHDRYGALSMDDRRQILCKLRKRNILMYRQLER
                     LKHDLLRLESKRVQCELEGNQTQVEVVETKILKKKEQFLKMLTQNKK"
     gene            543..2615
                     /locus_tag="IWO89_001830"
     CDS             543..2615
                     /locus_tag="IWO89_001830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:proton antiporter"
                     /protein_id="HAQ0456562.1"
                     /translation="MEFAELVIVFAVTITLSNIASRILPMIPAPLIQIFLGVVLGLTE
                     WGQSIDFEPELFLVMIIAPLLFREGEKADISSILKNFGTILFLAFGGVILTLVGVGAT
                     LSFLLPSVPLAACFAFGAALGPTDAVAVSSLSGRVNIPKKAMHILEGEGLLNDASGVT
                     AFQFALGALITGSFSAVNAGMSLVVSSIGGALIGFLLVWFKQKIIHLIEKASAQDVTG
                     YLLIELLLPFLAYVLAEAFDVSGIIAAVAAGILQASGFRKISVFDAELSSLSHSTWTT
                     IAFTLNALVFIFLGIELTQVFSPVWGDGLYPNGLLLAIIVLISVMLFVIRFISISLFY
                     VFKDGSKKFKKQLNEILILTFGGVKGTVSLATIFILPFSINNMMFYQRSLLLFLTAGV
                     ILVTLVIGIIVLPMLTETEEAKSTDLNALMILEEVVEVLRKEIKEIDRNTKEFLATEA
                     VIENYQERIRDLYLEDLTDDEKQEVQEIQALILSIERDGLDESYRSGKLSSNGYRFYS
                     RFLSRFEHSITSQILSFIGFWFIVVRRLMRIVLHPKMFWQRRYADRQTFISDEDIQEI
                     RETYQKNTQLIIESLDNLTDIYDETMVQFFIQQRKLEGIKMNSGNLISSWMIQQDSLF
                     TKKMLRGYYLERKIIDEYEVAEKITTFSANSYRRNINLLESYTMNKPSDNFSFRFAFR
                     AKSKKRVQ"
     gene            2780..4666
                     /locus_tag="IWO89_001831"
     CDS             2780..4666
                     /locus_tag="IWO89_001831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HAQ0456563.1"
                     /translation="MLKELKVTDLKKTYGEKDLFDQISFLVHEKDRIGLIGTNGTGKT
                     SLLNIIAGIDSSDGDRQTVFYPTDYRIGYLSQAAEFSDELTVLQAVFQGNSPLIQTVR
                     AYEEALIELGENGEDPDVQKRYAKAEEQMNKEDAWTTDTNAKIILQKLGISELDKKIS
                     TLSGGQKKRVSLAQVLIDEPDLLLLDEPTNHLDYEAIEWLENYLKQYRGALLMVTHDR
                     YFLDRVANRIFELSFGKLYEYKGNYEAYVLEKAERDRVEVEQEEKRKRLYKQELAWMR
                     AGVQARGTKQQARINRFEDLKENLYQVNQEDDLELNLATQRLGKKVLEIKDGSYRIND
                     QTLLEHLDLLIQSRERLGITGKNGAGKSTLLNILAGRIPLDSGTMSIGETVHLAYYTQ
                     ENEEMAPDKRMIAYLQEAAEEAKTADGSQIGVAELLERFLFPRFMHGTLIGKLSGGEK
                     RRLFLLKLLIQQPNVLLLDEPTNDLDIATLTILEDYFRSFPGAVITVSHDRYFLDKVA
                     DKLLVFQGNGKQELYYGNMSSYLLKQKETQQPAEKAKPKIQSKEPAEKKKLSYMEQKE
                     WETIEDEIAELEEKISLLQEEMNHQGDNFTRLQELQNDVSETEAQLEEKMARWEYLSE
                     WVED"
     gene            4678..5625
                     /locus_tag="IWO89_001832"
     CDS             4678..5625
                     /locus_tag="IWO89_001832"
                     /EC_number="2.1.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HAQ0456564.1"
                     /translation="MEQAYLDLGRKLLEEGHRKEDRTGTGTLSLFGYQMRFDLQKGFP
                     LLTTKRVPFGLIKSELLWFLKGDTNIRYLLQNKNHIWDEWAFERYIKSPDYNGPDMTD
                     FGHRCLTDPEFNEEYKKQSKLFCEKILTDDSFAETYGDLGHIYGYQWRHWETKDGGFI
                     DQIKQVIEAIKKTPDSRRLIVSAWNPEDVPSMALPPCHTMFQFYVQEGRLSCQLYQRS
                     GDVFLGVPFNIASYALLTHLIAHETGLAVGEFVHTLGDAHLYINHIEQMKEQLSREIR
                     AFPTLVLNQEKQSVFDFEMEDIQLEGYDPHPAIKAPIAV"
     gene            5645..6172
                     /locus_tag="IWO89_001833"
     CDS             5645..6172
                     /locus_tag="IWO89_001833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HAQ0456565.1"
                     /translation="MLAAIWAQDENGLIGKEDRLPWRLPNDLKFFKQMTEANTLVMGR
                     KTFEGMGSRPLPNRQTIVLTRDSSYKAEGVHVMHSVEDVLALEKETDGIFFIAGGSAV
                     YQEFLPFCTILYRTVIHHVFDGDAYFPPVDWSDWSLINTSQGEMDEKNRYPHQFETYQ
                     RNENPVKSGTGELTI"
     gene            complement(6235..6888)
                     /locus_tag="IWO89_001834"
     CDS             complement(6235..6888)
                     /locus_tag="IWO89_001834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin III family protein"
                     /protein_id="HAQ0456566.1"
                     /translation="MEKTKFNRTYEILNEVFNAVTHGIGTGLSIAGLVVLLVKGAHLH
                     SSLHIVSYAIYGSMMILLFLTSTLFHSLIFTKAKKVFQVFDHSSIFLLIAGSYTPFCL
                     LSIRGWLGWTLFVLIWLFAILGIIYKALTLHKRERVSKVSTIIYIFMGWLCVVAAVPL
                     YHSLGMTGVILMVAGGLSYTIGAFFYSLNSIRYMHVVWHLFVMLGAGFMYFSVLFFT"
     gene            7020..7862
                     /locus_tag="IWO89_001835"
     CDS             7020..7862
                     /locus_tag="IWO89_001835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HAQ0456567.1"
                     /translation="MTNVKIVTDSSCTMEKNVRDALGIRVMPLSIMIDGTVYPDDDQL
                     DGERFMEMMAKAKNLPKTSQPPIGEFISMYDELGEDGSEVISIHMTKGLSGTVEAARQ
                     ASNLTKTKVTVIDSDTTDQGLSFQVIRAAELAKEGKTVEKILEEVKKVQDNTKLYIGI
                     STLDNLVKGGRISRTTGLLSSVLNIRVVMNFAHSELIPVTKGRGKKTFDKWFNNLKNE
                     LKELPNVRQIGISHADAKELAQEFKEGLQELFPDMHIPVLHTNPVIATHTGKDAFAIM
                     YYVD"
     gene            8020..8916
                     /locus_tag="IWO89_001836"
     CDS             8020..8916
                     /locus_tag="IWO89_001836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HAQ0456568.1"
                     /translation="MKLTKRHLILVLFPLLIAIIIYAGLAASIPKAGPLMKPVTTVRE
                     DPNKKEKLHYIAIGDSLTEGVGDQTKQGGFVPLVANDLKDRYNLTAVETENYGVNGER
                     SDQILKRVKTKEEIRNNIKTADFITLTVGGNDLMKVIQNNLFGLTVNSFKKPLKNYQE
                     NVTELLEEFRSLNKTAPIYILGIYNPFYLNFPEITDMQTIVNNWNKGTETVTKKMPNC
                     YFIPINDLLYQGLNQEVGVTEEGTDQTTSTTENLNGIKNNVLYEEDHFHPNNLGYQLM
                     ANAVRDQLIETQNQWLEKEDDQ"
     gene            8919..9632
                     /locus_tag="IWO89_001837"
     CDS             8919..9632
                     /locus_tag="IWO89_001837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YpmS family protein"
                     /protein_id="HAQ0456569.1"
                     /translation="MKTRSDVSSNKTKKTNYWKFAFLILLGVVLGSTVFLGSRIFANR
                     EPELPEIPALTERQGDPVLTINSNKEKVNQIISFFLSEYQKDSDIEYKFYLENEALLN
                     GTFEVLGFPIDFYLYFDPYVMENGNVQLKARSLSIGTLSLPIKDVMNMIKRNYKLPDW
                     IEVNTDDLTIMLRLDQFRMQNGMYIKADKIDLVNDDIRFSLYLPKDTSDASNNSNTNK
                     TSTSSTESKTKENKKSSDE"
     gene            9652..10170
                     /gene="msrA"
                     /locus_tag="IWO89_001838"
     CDS             9652..10170
                     /gene="msrA"
                     /locus_tag="IWO89_001838"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HAQ0456570.1"
                     /translation="MERAIFAGGCFWCMIQPFDTLPGIHTVMSGYTGGHVPDPTYEQV
                     KSQTTGHTEAVEIIYDPTLISYEQLVELYWQQTDPTDAFGQFEDRGDNYRPVIFYLTE
                     EQRIIAEKSKKQLAESGRFEQPIVTKIEPAQPFYLAEEDHQEYYKKHPENFKRNHARR
                     AAFIEENWRNEG"
     gene            10167..10394
                     /locus_tag="IWO89_001839"
     CDS             10167..10394
                     /locus_tag="IWO89_001839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YozE family protein"
                     /protein_id="HAQ0456571.1"
                     /translation="MNRSFFHYLMTLRGGGTHDALSVFATEAGKDIQFPKHSSSYEEI
                     SNYLELNVDYLPSMDIFDEAWEKYLENNHDI"
     gene            10546..11097
                     /gene="lepB"
                     /locus_tag="IWO89_001840"
     CDS             10546..11097
                     /gene="lepB"
                     /locus_tag="IWO89_001840"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HAQ0456572.1"
                     /translation="MTKKQRYINRFWLVFKYLLVSVFLAFMLRGFLFIPVPVEGNSME
                     NVLKQGDMVVMEKFSEIRRFDIVVFQLADGTIYIKRVIGLPGENVSYQNDQLKINGKV
                     VKEPYLTKNLKSDHANASYTTDFTLQELTGQSKLPEDNYFVLGDNRRVSKDSRSFGTI
                     NKTDILGKARFVYYPLDEIKWIQ"
     gene            complement(11180..11722)
                     /locus_tag="IWO89_001841"
     CDS             complement(11180..11722)
                     /locus_tag="IWO89_001841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="folate family ECF transporter S component"
                     /protein_id="HAQ0456573.1"
                     /translation="MNTLIAKDVFRLPEMNARLLSTSALLLALDVILYKIAIGPAYFQ
                     ITFGFLSMALMGYLYGPIWAGLLEMLSNTLNFTIFGSGTFQAAFLITAFLGGFINGLF
                     LYHKKINLPSVIAAQLLIMIPVSLFINSWLMSVLFGADFKVIFLGRFIRNMIQIPIQI
                     MVVYSFIKTLEARKVFKRAV"
     gene            complement(11959..12357)
                     /locus_tag="IWO89_001842"
     CDS             complement(11959..12357)
                     /locus_tag="IWO89_001842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxalase"
                     /protein_id="HAQ0456574.1"
                     /translation="MNSMVFVNFPVADVNRSVAFYEKLGFKKNEEFSTEEAGSMVWDD
                     TFWIMLLSHDFYKKFIKDKEIADNKKTSGALISFSVENADEVKKFAQIAKENGGTFHE
                     VDMGMPEEEMFSLEVQDLDGNTLEPVWMKM"
     gene            12617..13477
                     /gene="ylqF"
                     /locus_tag="IWO89_001843"
     CDS             12617..13477
                     /gene="ylqF"
                     /locus_tag="IWO89_001843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase YlqF"
                     /protein_id="HAQ0456575.1"
                     /translation="MTIQWFPGHMAKARREVSEKMKYVDIVFELVDARLPLSSRNPML
                     DQIIQQKPRLVLLNKADLADPQQTKLWQEYFRKQGHLALAITAQESKGIKGLVPAAKE
                     ALKEKRARDAARGIKPRAIRAMVLGIPNVGKSTLMNRLVGKKIAQTGNKPGVTKGQQW
                     LRLGNELELLDTPGILWPKFEDEEIGKKLALTGAIKDQLVHLDDLAIYGLDFFARYYS
                     GRISDRYGLSIEQEEKLAPELLMDITAKRGYKEDYDRGSELVVHDIRQGKLGRYTLDR
                     CEEMEQKSDD"
     gene            13470..14237
                     /locus_tag="IWO89_001844"
     CDS             13470..14237
                     /locus_tag="IWO89_001844"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HAQ0456576.1"
                     /translation="MTESIQSIKERLKSIQRIDDPTLESLRNDPRKGVQQALASFERR
                     LKKEALIKEKYEEMFCFEKQGYAQGHQWIAGIDEVGRGPLAGPVVAAAVILPKNEIIL
                     GLNDSKQLSEKKRELLYQQVQEKALAIGIGIVDQQTIDDVNIYEASKLAMIQAVEKLS
                     IVPSYLLIDAMTLDLPIQQEKIIKGDARSVSIAAASIIAKVYRDQLMKEYDVLYPGYG
                     FSHNAGYGTKEHLEGLERQGITPIHRKSFAPVKKYLQ"
     gene            14293..15150
                     /gene="dprA"
                     /locus_tag="IWO89_001845"
     CDS             14293..15150
                     /gene="dprA"
                     /locus_tag="IWO89_001845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-protecting protein DprA"
                     /protein_id="HAQ0456577.1"
                     /translation="MYQIEENLLKLAYTKGISSQGKWALANWMMQYPYKEIGFEDIIK
                     IGKITKHRELFRKSWQEIHANWGKIQSKQSFITCFDPKYPPQLLHLTYPPIVLFYNGD
                     LSLLSCNMLSVVGGRHTSALAKKTVYYLLKPVIEAGYVIVSGCAKGIDTYGHQAAISH
                     GGRTIAVIGTGIEQVYPAENRDLQEVIANDHLLLSEYPPQSGPKKHCFPMRNRIIAAL
                     SKGTCVFEAKKKSGSLITAQQALDLGRTVFSVPGNILTEHSTGCHHLIQDGAVCTVSG
                     EDILAEFKE"
     gene            15272..17350
                     /gene="topA"
                     /locus_tag="IWO89_001846"
     CDS             15272..17350
                     /gene="topA"
                     /locus_tag="IWO89_001846"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002380330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I DNA topoisomerase"
                     /protein_id="HAQ0456578.1"
                     /translation="MAYKYLVIVESPAKAKTIEKYLGRNYKVMASVGHIRDLPKSKMG
                     IDFENNYEPHYISIRGKGDVIKSLKAAAKKAQKVYLASDPDREGEAIAWHLAYLLGLD
                     LKEKNRVVFNEITKDAVKAAFKEPRTIDVDLVDAQQARRTLDRIVGYSISPILWRKVK
                     KGLSAGRVQSVALKIIIDREKEIREFVPEEYWSIDGNFKKARKKFKANFWGIDGKKKK
                     LPDAQSVKEVTARIDGTDFEVKKVEKKERKRNPANPFTTSSLQQEAARKLNFRTRKTM
                     MVAQQLYEGITLGKQGTVGLITYMRTDSTRIADTAKAEAAAFIEDTYGKEFSRHDHRK
                     IKNAQGAQDAHEAVRPTSVLRTPDELKQYLDKDQLKLYTLIWSRFVASQMTPAILDTM
                     KVTLEQNGVIFIANGSKIKFKGFMQVYVEGRDDGKEEKENILPELEEGDVVQSVDIEP
                     KQHFTQPPARFSEATLIRALEENGVGRPSTYAPTLDTIQRRYYVKLTQKRFEPTELGE
                     IVNSLICEFFPQIVDIHFTAEMEGDLDKIEEGTEAWVKVVDRFYKPFEKELTNAEEKI
                     EKIQIKDEPAGFDCDVCGHPMVIKLGKYGKFYACSNFPDCRNTKPIVKEIGVTCPVCH
                     EGQVIERKSKKNRLFYGCSRFPECEFTSWDKPVGRNCPKCDGYLVEKKVKGGKQVVCV
                     NGDYEEDIQK"
     gene            17319..18695
                     /gene="trmFO"
                     /locus_tag="IWO89_001847"
     CDS             17319..18695
                     /gene="trmFO"
                     /locus_tag="IWO89_001847"
                     /EC_number="2.1.1.74"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FADH(2)-oxidizing
                     methylenetetrahydrofolate--tRNA-(uracil(54)-C(5))-
                     methyltransferase TrmFO"
                     /protein_id="HAQ0456579.1"
                     /translation="MEITKKTSKNNRKEVLSLKMVNVVGAGLAGSEAAWQIAQAGVPV
                     RLYEMRPVKSTEAHHTSNFAELVCSNSLRGNSLANAVGLLKEEMRRLNSVVITSADET
                     AVPAGGALAVDRDSFSETITRKVKEHPLVTVINEEITEIPEGITVVATGPLTSHPLAE
                     SIKAFNGSDGFYFYDAAAPIVDKATIDMDKVYLKSRYDKGEAAYLNCPMTEEEFKAFH
                     EALINAEVAELKSFEKEKYFEGCMPIEVMAQRGFKTMLFGPMKPVGLEDPKTGKRPYA
                     VIQLRQDNAAASLYNIVGFQTHLKWGEQKRVFRMIPGLENAEFVRYGVMHRNSFMNSP
                     ELLKPTYQSTKREDLFFAGQMTGVEGYVESAASGLLAGINAARLAKEMEPVEFPQETA
                     IGSMAYYITHAEGKHFQPMNANYGLFPELPERIRDKKARYEAIAQRALKANEAIKTEL
                     EKTAIPNE"
BASE COUNT         6518 a         3231 c         3899 g         5091 t
ORIGIN      
        1 tttgtcgcag ccccattttt ttgttgtgat aaaagaacaa gaaaggcagg aatgggaaag
       61 taaacatcct tttgtcagaa acttttatct ttcttaagtt ttacttgata tctgatacta
      121 tttggaatag aatgaggcgt tgaaaaattt atggaaattt ctaactagca ataaacgatg
      181 aaacaatgaa atatttttca tccaaaaaga actttgctat aatgatggaa gtttgtgtat
      241 aggagatctg gaaaatgaga gagagttttg aacaacaaaa aaaactgttg catgatcgct
      301 atggcgccct ttcgatggat gaccgccgtc aaatactttg taagttgcgt aaaagaaata
      361 tattgatgta tcgtcagttg gaacgtctga aacatgatct gcttcgtttg gagtcaaaac
      421 gtgtacagtg tgaattagag gggaaccaaa cacaggttga agtagtcgaa accaaaattt
      481 taaagaaaaa agaacaattt ttaaaaatgc ttacacaaaa taaaaaataa aggggatgga
      541 cgatggaatt tgctgagtta gttattgtat ttgcagtaac gatcacctta tcgaacatag
      601 ctagccgcat tttgccaatg attccagctc cactgatcca aattttcctt ggagtggtat
      661 taggtctgac agagtgggga caatcaatcg attttgaacc tgaattgttc ttagttatga
      721 ttattgcacc attgttattt agagaaggag aaaaagcaga tatttcttcg attctcaaaa
      781 atttcgggac aattttgttc ttggcatttg gaggagtcat tttgaccctt gtcggggtag
      841 gtgcgacgtt atcattcctg ttgccaagtg tcccacttgc tgcctgcttt gcttttggag
      901 cagcgttagg tccgacagat gcggtagcag tcagttctct ttctggacga gtgaatatcc
      961 ctaaaaaggc gatgcatatt ttggaaggag aaggtctttt gaatgatgca tctggtgtta
     1021 ctgccttcca gtttgctctt ggtgcgctga tcaccggatc tttttcagca gtaaacgcgg
     1081 ggatgagtct agtagtttct agtattgggg gagctttgat cggctttttg cttgtctggt
     1141 tcaaacaaaa aataattcat ttgatcgaaa aagcatcagc acaagatgtc acagggtatc
     1201 tgctgatcga acttttgctt ccttttctag cctatgtatt ggcagaggca tttgatgtat
     1261 ctggaatcat tgctgcagtg gcagcaggta ttctccaagc ttctggtttt aggaaaatct
     1321 ctgtgtttga tgcagaattg tcgagcttgt cccacagtac atggacaact attgctttta
     1381 cactgaatgc attagtattt atatttttag gaatcgaatt gacgcaagtg ttctcgccag
     1441 tatggggaga cggtttatat ccaaacggtc ttttattagc gattatcgta ttaatctctg
     1501 tcatgctttt tgtgatcaga ttcatttcta tcagtctttt ttatgtattc aaagatggca
     1561 gtaaaaagtt caaaaaacag ttgaacgaga tcttgatctt aacatttggc ggtgtaaagg
     1621 gaacagtcag tttggctacg atttttattt tgccattttc aatcaataat atgatgtttt
     1681 accagcggtc tttgcttttg tttctgacgg cgggtgtcat cttagtgact ttggtcatag
     1741 ggatcattgt cttgcctatg ctgacggaaa cagaggaagc gaaaagcacg gatttgaatg
     1801 ctttgatgat tttagaagag gtcgttgaag ttttgcgaaa agaaataaag gaaattgatc
     1861 gtaatacaaa agaattttta gccactgaag cagtgataga aaattatcaa gaacggatac
     1921 gtgatctgta cttggaagat ttaaccgatg atgaaaagca agaagtacaa gaaatacaag
     1981 cactgatttt gtcgatcgaa cgggatggat tggatgaaag ttatcgttcg ggcaagcttt
     2041 cttctaatgg ttatcgtttt tactcccgtt ttctttctcg attcgaacat tctatcacaa
     2101 gccagatttt gtcttttatc ggcttttggt tcattgttgt ccgtaggtta atgcgaatcg
     2161 ttctccatcc gaaaatgttt tggcaaagaa gatatgctga tcgtcagaca tttattagtg
     2221 atgaagatat ccaagaaatc agagagacat atcaaaaaaa tactcagttg attattgaaa
     2281 gtttagataa cttgactgat atttatgatg aaacgatggt tcaatttttt atccagcaac
     2341 gcaaacttga aggaataaaa atgaactcgg ggaatttgat ttcttcgtgg atgatccagc
     2401 aagatagtct gtttacgaaa aaaatgctaa gaggttatta tcttgaaaga aaaatcattg
     2461 atgaatacga agttgctgaa aaaatcacga cgttttctgc gaacagctat cgcaggaata
     2521 tcaatttgtt agaatcttat acgatgaaca aaccatctga taacttttct ttccgatttg
     2581 cgtttcgagc aaagtcaaaa aaacgagtcc aataaaataa aaaggtaaaa attttcaagt
     2641 tttataaaaa acaaaacata tcagaacagg atcgaatcgg tcctgttctt tttttgagaa
     2701 cacaaaaaga acaaatacca aaaaaatttt cctgaagttc actattttta tgatacaata
     2761 tccaagataa ggagggaaca tgttgaaaga attaaaagta actgacctca aaaaaactta
     2821 tggtgaaaaa gatttgtttg accagatttc ttttttggtc cacgaaaaag accgtatcgg
     2881 gttgattggg acgaacggga cgggtaaaac aagtctctta aatattattg ccggtatcga
     2941 tagcagcgac ggtgatcggc aaacagtgtt ttatccaact gattaccgta ttggttatct
     3001 ttctcaagca gctgaatttt ccgatgaatt aactgttctc caagcagtct ttcaaggaaa
     3061 cagccccttg atccaaactg ttcgagcata tgaggaagca ttgatcgaac taggggaaaa
     3121 cggagaagac cctgatgtgc aaaaacgtta tgctaaagca gaagaacaaa tgaataaaga
     3181 agatgcttgg actacagata ccaacgcaaa gattattttg caaaaattag gaatcagtga
     3241 attagataaa aaaatcagta ctttatcagg tggtcagaaa aaacgagtta gtctggcgca
     3301 agtgttgatt gatgaacccg atttactatt actagacgaa ccaacaaacc atttagatta
     3361 tgaagcgatc gagtggttgg aaaactattt gaaacaatac cgtggcgcac tattgatggt
     3421 tacccacgat cgttattttc tagatagagt agcaaaccga atcttcgaac tttcatttgg
     3481 aaaattatac gagtataaag gaaattatga agcttacgtc ttagaaaaag cagaacgtga
     3541 tcgtgtagaa gtagaacaag aagaaaagcg caagcggctt tataaacagg aattagcttg
     3601 gatgagggct ggcgtacagg cacgtggcac caagcagcaa gcacggatca accgtttcga
     3661 agatctaaag gagaacttgt accaagtaaa ccaagaagat gatttagagc tgaacctagc
     3721 gacacaacgt ttagggaaaa aagtactgga aatcaaagat ggcagttacc gtataaatga
     3781 ccaaacactg ctagaacatt tagatttatt gattcaatct cgggaaagac taggtattac
     3841 tggtaaaaat ggtgcgggaa aatcgacatt gcttaatatt ctagctggca gaattcccct
     3901 ggatagcggt acaatgtcta tcggtgaaac ggttcatctt gcgtattata cacaagaaaa
     3961 tgaagaaatg gcaccagata aacggatgat tgcttactta caagaagctg ctgaagaagc
     4021 aaaaacagca gatggcagtc aaatcggagt agcagaatta cttgaacgat ttttatttcc
     4081 aagatttatg catgggacac tgataggcaa actttcaggc ggagaaaaac gccggttatt
     4141 tttgttgaaa ctgctgattc agcaacctaa tgttttatta ttggatgaac cgaccaatga
     4201 tttggacata gcaacattga cgattttgga agattatttt agaagttttc caggtgctgt
     4261 gatcactgtt tcccatgacc gttatttctt ggacaaggta gcagataagt tattagtatt
     4321 ccaaggaaac ggaaaacaag aactttatta cggaaacatg tccagttatt tactgaagca
     4381 aaaagagacc cagcagcctg ctgaaaaagc gaaaccaaaa atacagagca aggaacctgc
     4441 tgaaaagaag aaattatctt acatggaaca aaaagaatgg gaaacgattg aagatgagat
     4501 tgcagaactt gaagaaaaaa tctcattgct ccaagaagag atgaatcatc aaggagataa
     4561 tttcactcga ttacaagaat tgcaaaacga cgtatctgaa acagaagcac aacttgaaga
     4621 aaaaatggca cggtgggaat acttgagtga atgggtagaa gattaggagg aaaaaggatg
     4681 gaacaagcgt atcttgactt aggaagaaaa ttgttggaag aagggcatcg taaagaagac
     4741 agaacaggca caggcacatt aagcttattc ggataccaaa tgcggtttga tctgcaaaaa
     4801 gggtttcctt tgttaacaac gaaacgagta ccttttggat tgatcaaaag cgaattgctt
     4861 tggtttttaa aaggagatac gaatatccgc tatctattac aaaataaaaa ccatatttgg
     4921 gatgaatggg catttgaacg ctacatcaaa agtccagact ataatggacc agatatgact
     4981 gattttggcc atagatgttt gacagatcct gaattcaatg aagaatataa aaagcagagc
     5041 aagctttttt gcgagaagat cctaaccgat gattcctttg ctgaaacata cggcgatctt
     5101 ggacacatct acggctatca atggcgtcac tgggaaacaa aagacggtgg ctttatcgat
     5161 caaatcaaac aagtgatcga agcaatcaaa aagactcctg attctagaag attgatcgtt
     5221 tctgcatgga atccggaaga tgttcctagc atggctttgc ctccttgtca tacgatgttt
     5281 caattttatg ttcaagaggg acggctaagc tgccaattgt atcaacgcag tggggatgtg
     5341 tttctaggcg ttccgttcaa cattgcaagt tatgcgttgc tgactcacct aattgcacat
     5401 gaaacaggtt tagcggttgg cgaatttgtt cacacattag gagatgctca tctttatatc
     5461 aatcatatcg aacaaatgaa agaacaattg agccgagaaa tacgcgcatt tccgacttta
     5521 gttttgaatc aggaaaagca gtcagtattt gattttgaga tggaagatat ccaattagaa
     5581 ggatatgacc cacatccggc gatcaaagct cctatcgctg tttagaatca gaaaggatga
     5641 gaaaatgtta gctgcaattt gggcacagga tgaaaacgga ttgattggaa aagaagaccg
     5701 tcttccttgg cgtttgccaa atgatctgaa attcttcaaa caaatgacag aagcaaatac
     5761 actagtgatg ggacgaaaaa cattcgaagg gatgggtagc cgtccgttgc cgaatcgtca
     5821 gacgattgtt ttgacgagag attcttccta taaagcagaa ggcgtgcatg tcatgcatag
     5881 tgtggaagac gtgcttgctc tggaaaaaga aacagacggt atctttttca tcgctggagg
     5941 gtcagctgtt tatcaagaat ttctgccatt ttgtacgata ttataccgta cagtcatcca
     6001 tcatgtattc gatggagatg cttattttcc acctgttgac tggtctgatt ggtcactgat
     6061 caatactagt caaggagaaa tggatgaaaa aaatcgttat ccgcatcaat ttgaaacgta
     6121 tcagcgtaat gagaatcctg taaaaagcgg gacaggcgaa ttgacgatat aaaaaagcta
     6181 tggatagact agctgttatt tagagctgtc ccatccatag ctttattttt tttattaagt
     6241 aaagaataaa acagagaagt acataaaacc tgcacctaac ataacaaaca aatgccaaac
     6301 aacatgcata tagcggatag aattcaagct ataaaagaat gcgcctatcg tgtaagaaag
     6361 cccgcctgct accatcaaaa taacaccagt cattccaaga gagtgataaa gaggaacagc
     6421 agccacgaca cagagccacc ccataaaaat atagatgatc gtagatactt ttgatactct
     6481 ctcccgttta tgcaacgtca acgctttata tataatgcca agaatcgcaa acaaccagat
     6541 taatacaaat aaagtccaac ctagccatcc tcggatactc agcaaacaaa aaggagtata
     6601 acttcctgca atcaaaagaa aaatcgaact atgatcgaat acttggaaca ccttttttgc
     6661 ttttgtaaaa atcaatgaat gaaacaaagt agacgttaaa aataaaagaa tcatcatcga
     6721 tccatagata gcataagaga caatatggag ggaggaatgc aagtgggcac cttttactaa
     6781 aagaaccact agtcccgcga tactcaatcc agtcccaatt ccatgggtga ctgcattaaa
     6841 cacttcattt aatatttcat acgttcgatt aaatttcgtt ttttccatga aaacggctcc
     6901 tttactaggt gtaattaatg aaaaatcatt atttgtctgt tatactaaaa atagaaattg
     6961 tcttacatgt agtttaacat gaataaggca tcttgttaaa gaaagagaga gagaaaataa
     7021 tgacaaatgt aaaaatcgta acagattctt catgtacgat ggaaaaaaat gtacgcgatg
     7081 ctttaggcat tcgtgtcatg ccgctatcca tcatgatcga cggaacagta tatcccgatg
     7141 atgaccaact agacggcgaa agatttatgg aaatgatggc gaaagcaaaa aatctgccca
     7201 aaacaagcca accaccaatt ggagaattca tctcgatgta tgatgagcta ggagaagacg
     7261 gcagtgaagt gatttcgatc catatgacaa aaggcttgag tggaacagta gaagcagcaa
     7321 gacaagccag taatctaaca aaaacgaaag taacggttat cgacagtgat accactgacc
     7381 aaggattatc ttttcaggta attcgagcag ctgaattagc aaaagaaggt aaaacagtag
     7441 agaaaatcct tgaagaagtc aaaaaagtac aagacaatac aaaattatac atcggaatat
     7501 ccacactaga taacttggta aaaggtggaa gaatcagccg aacaactggt ttgctgtcta
     7561 gtgttttgaa catacgcgtg gtgatgaatt tcgcccattc tgaattaatc cctgtaacta
     7621 aggggcgagg aaagaaaacg ttcgataaat ggtttaataa tttgaaaaat gaactgaaag
     7681 aattgccaaa tgtgcgacaa atcggtattt cacatgcgga tgcaaaagaa ttggcgcaag
     7741 aattcaaaga gggtttgcaa gaattattcc cggacatgca tatccctgta ctgcatacga
     7801 atccggttat cgctacgcat actggtaaag acgcgtttgc aatcatgtat tatgtagatt
     7861 agtcgaaaag aggataagcc ctcagcctga aaggcttgca gcttattctc tttttcttta
     7921 taaaattagg aaaataattg ttttcttttt tcataaagac tacctttttg ataaaatttt
     7981 tgataacatt agaaccaatc gaatacaaga ggtgaaaaaa tgaaattaac gaaacgacat
     8041 ttgatcctcg ttttgttccc attattaatt gcgatcatca tctatgcagg tttagctgct
     8101 tctataccga aagctggccc actaatgaaa ccagtaacta ctgtgagaga agatccaaat
     8161 aaaaaagaaa aacttcacta catagctatc ggagattcgc tgactgaagg agtaggagat
     8221 caaaccaagc aaggaggatt cgttcccctt gtcgcaaatg atctaaaaga ccgttataat
     8281 ttgaccgcgg ttgaaacaga aaactatggt gtaaatggtg aacgaagtga ccagatctta
     8341 aagcgagtaa aaacaaaaga agaaattcga aacaatatca aaacagctga ttttatcaca
     8401 ctgactgttg gcggtaatga tttgatgaaa gttatccaaa ataatttatt cgggttaacg
     8461 gtcaattcct ttaaaaagcc actgaaaaac tatcaggaaa atgtcacaga actattggaa
     8521 gaatttcgat ccctcaataa aaccgcacct atttacattt taggcatcta caatccattt
     8581 tatttgaact ttcctgagat cactgacatg cagacaattg ttaacaattg gaacaaagga
     8641 acagaaacgg tgacaaaaaa aatgccgaac tgctacttta tcccaatcaa tgatttgctt
     8701 tatcaaggat tgaatcagga agtaggtgtc acagaagaag gaacagatca aacaactagc
     8761 acgacagaaa atctaaatgg catcaaaaat aatgtgctct atgaagaaga tcatttccat
     8821 ccaaataatt taggttatca attgatggca aatgcagtga gagatcagct gatcgagaca
     8881 caaaaccaat ggttagagaa ggaggatgat caataaacat gaaaacaaga tctgacgtat
     8941 cttcaaacaa aacaaagaaa acaaattact ggaaattcgc cttccttatc cttcttgggg
     9001 tagtacttgg ttcgaccgta tttttaggca gccgtatttt tgctaatcgg gaaccagaac
     9061 tcccagaaat accagctctt acggaaagac aaggagatcc tgtcttaacg atcaattcaa
     9121 ataaagaaaa agtcaatcaa atcatcagct ttttccttag tgagtatcaa aaagatagtg
     9181 atattgaata taaattttat ttggaaaatg aagcactact caatggtact ttcgaagtac
     9241 taggttttcc tatcgatttc tatttatatt ttgatccgta tgtgatggaa aacggaaatg
     9301 tccaactaaa agctagaagt ctttctatcg gaacattgag tttgccaatc aaagatgtga
     9361 tgaatatgat caagagaaac tataaattac cagattggat cgaagtcaat acagatgatc
     9421 tgactatcat gcttaggttg gatcaattcc gaatgcaaaa cgggatgtat attaaagcag
     9481 ataaaatcga cttagtaaat gatgacattc ggtttagtct atatttgcca aaagatacct
     9541 ctgatgcttc aaacaactca aataccaaca aaactagcac cagcagtact gaaagcaaga
     9601 caaaagaaaa taaaaaatca tcggatgagt agtattagag ggggaatcat aatggaacga
     9661 gcaatatttg caggaggctg cttctggtgt atgatccagc ctttcgacac gcttcctggt
     9721 atccatacag tgatgtcagg atatactgga gggcatgtac cagatccgac ttatgaacaa
     9781 gtaaaaagcc aaacgactgg acatacagaa gcagtggaga ttatctatga tccaacattg
     9841 atttcatatg aacaattagt agaactttac tggcagcaaa ctgatccaac tgacgctttc
     9901 ggacaattcg aggatcgcgg tgacaattat cgcccggtga tcttttattt gacagaagaa
     9961 cagcggatca ttgctgaaaa aagtaagaaa cagttagctg aaagtggacg tttcgaacag
    10021 ccaatcgtta caaaaatcga accagcccaa ccattttatc tagcagaaga agaccatcaa
    10081 gagtattata aaaagcatcc tgaaaatttt aaaagaaacc atgcacgccg ggctgccttt
    10141 atcgaagaaa attggaggaa cgaagggtga atcgaagctt ttttcattat ttaatgacac
    10201 tgcgaggcgg tggcacacat gatgctttat ctgttttcgc tacagaagcc ggtaaagaca
    10261 tccaatttcc taagcacagc tctagctacg aagaaatttc taattatctg gaactgaatg
    10321 tcgactattt gccgtccatg gatatctttg acgaagcatg ggaaaaatat ttagaaaata
    10381 atcatgacat ataagagata gttttgtact ttatgatcaa aaaagttttt tgtccaatag
    10441 cctaattgaa agaattattt ttatttcagc tatctagtcg catttttttg atatttttct
    10501 tataataaag aaagaatgaa aaaaagaggg atggattgat agagattgac aaaaaaacaa
    10561 cgctatatta atcgcttctg gcttgtattc aaatacttac ttgtttctgt attccttgcg
    10621 ttcatgttgc ggggattttt attcatcccg gtaccagtgg aaggcaattc aatggaaaac
    10681 gtgttgaagc aaggtgatat ggttgtgatg gaaaaatttt cggagatcag acgatttgat
    10741 attgttgttt ttcaattagc agatggcacg atttatatca aacgagtaat cggtcttcca
    10801 ggagaaaatg tcagctacca aaatgatcaa ttgaagatca atgggaaagt agttaaggaa
    10861 ccttatctaa ctaaaaacct gaaatcagat catgcaaatg catcctatac gacggacttt
    10921 acactacaag agctaacggg acaatccaag cttccggaag ataattattt tgtacttgga
    10981 gataatcgaa gagtttcaaa agatagtcgt tcgtttggca cgatcaataa aactgatatt
    11041 ctaggtaaag caagatttgt ctattatcca cttgatgaaa taaaatggat ccagtgagaa
    11101 acaataaagc gtgcagtctg ttgagactgc acgctttatt gtttaatcca aaatcagttt
    11161 actaccttag tacgtaaaat caaacagctc gtttaaacac ttttctagcc tctaaagttt
    11221 tgataaatga ataaacaacc attatttgaa tagggatttg gatcatattc ctgatgaatc
    11281 tacctagaaa aattactttg aaatctgcac caaacaaaac agacatcagc caagaattga
    11341 taaaaaggct gactgggatc ataatcaata attgggcagc gatgacggaa ggcaggttta
    11401 tctttttatg atataaaaat aagccattaa taaatccacc aaggaaagcc gtgataagaa
    11461 aagcagcttg aaacgttcct gaaccaaata tcgtgaaatt taaagtgttc gacaacattt
    11521 caagcaaacc agcccagatc ggtccatata gataacccat aagcgccata cttaaaaatc
    11581 cgaatgtgat ttgaaaataa gcaggaccaa tcgctatctt gtataagatg acatcaaggg
    11641 ctaataaaag agcagaggtc gacaacaatc ttgcattcat ttcgggcaat ctaaaaacgt
    11701 cttttgcaat caaagtgttc aaaatattct ctcctttgtc agttaataat aaaaggatac
    11761 cataaaatca ccaatgtgtc atgacacatt ggtgatttac atacactggc atctaacaat
    11821 taaaaaaagg gtgagaagac tgcttttata aactgtaaaa aagagattgt acgaataaaa
    11881 aaaatagagg cggagaatac ttatcgtatc atccgccttt gtttttttgt tataaaaaac
    11941 gtcagattag ttatctgctt acatcttcat ccatacgggt tctaatgtat ttccgtctag
    12001 atcttggact tctaatgaaa acatttcttc ttcaggcatt cccatatcta cttcgtggaa
    12061 agtaccacca ttttcttttg caatttgagc aaattttttt acttcatccg cgttttcaac
    12121 actaaacgaa atcaaagcac cacttgtttt tttattatca gctatttctt tatcttttat
    12181 aaatttttta taaaaatcat gagagagaag cattatccaa aaagtgtcat cccataccat
    12241 tgaaccagct tcttcagtag aaaattcttc atttttttta aaacccagct tttcataaaa
    12301 agcaacggaa cgattgacat cagcaacagg aaaattaaca aaaaccatac tattcatttg
    12361 tttcgtctcc tttctgtttt gttcgtctat catctcttat ttaggaaacg ctgtcaaaaa
    12421 tttagattca tatatagaaa ccaaaataaa aatttgcatc atcaaaaaag tgaagatgtg
    12481 atagaatgag gactggttca ctttcactta actaatagat ggaatttctt tataataaaa
    12541 aagtgaattt atattgaaaa agaaaaaaac aagtaaagcc ggggaatatt ccggtatgaa
    12601 aggaatggag agaacaatga ccatccaatg gtttccggga catatggcaa aagcccgacg
    12661 agaagtttct gaaaaaatga aatatgtgga catcgtattt gaattggtag acgcacgatt
    12721 acctttatct tctcgtaatc caatgctaga tcagatcatt cagcaaaaac ctcgccttgt
    12781 tttattgaat aaagcggatc tggcagatcc acaacagaca aagctgtggc aagaatattt
    12841 tcgtaaacaa ggccatcttg cattagctat cacggcacaa gaaagtaaag gaattaaagg
    12901 tctagtacct gcggctaaag aagcattgaa agaaaaacgg gcaagagatg ctgctcgggg
    12961 gatcaagcct cgcgctattc gggcaatggt cttaggtatt ccaaatgttg ggaaatctac
    13021 gttgatgaat cgactagtag gcaagaaaat tgcacagaca ggaaataagc ctggcgtaac
    13081 aaaaggtcag cagtggcttc gtttaggtaa tgagttggaa ttgttagaca caccagggat
    13141 tttatggccc aaatttgaag atgaagaaat cggcaaaaaa ctagcgctga caggcgcgat
    13201 caaagaccag cttgtccatt tagatgattt agcgatttac ggattagact tttttgcccg
    13261 atattattca ggaagaatca gcgatcgtta tggtttatca atcgagcaag aagaaaaatt
    13321 ggcaccggaa ttactgatgg atattacagc aaaacgtggg tataaagaag actatgatcg
    13381 tggcagcgaa ttagtcgtgc atgatattcg tcaaggaaaa cttggacgat acacactgga
    13441 tcgttgcgaa gaaatggagc aaaaaagtga tgactgaatc catccaaagt atcaaagaac
    13501 gattgaaaag catacagaga atagatgatc ctacactaga atcattaaga aatgatccac
    13561 gcaaaggcgt gcagcaagca ctggcttctt ttgaacgacg attgaaaaag gaagcattaa
    13621 ttaaggaaaa atatgaagaa atgttttgct ttgaaaagca agggtacgca caaggtcacc
    13681 aatggattgc cggcattgat gaagtaggaa gaggaccatt agctggacct gtcgtggcag
    13741 ctgcagttat cttgccgaaa aatgagatca tattagggct gaatgactcc aaacaattat
    13801 cagagaaaaa gcgagaactt ctttatcaac aagttcaaga aaaagccctg gcaatcggga
    13861 tcgggatcgt tgatcagcag acaatcgatg atgtgaatat ttatgaagca agtaaattag
    13921 cgatgatcca agcagtagaa aaattgtcca ttgtaccaag ctatttgttg atcgatgcga
    13981 tgaccttgga tttgcctatt cagcaggaaa aaataatcaa aggggatgca cgatctgtgt
    14041 cgattgctgc tgcaagtatc attgctaaag tatatcgaga ccaattgatg aaagaatatg
    14101 acgtgcttta tcctggatat ggtttctcgc ataatgcagg ttatggaaca aaggaacact
    14161 tagaaggact tgaacgacaa ggaattacgc caatccatcg taaaagtttt gcacctgtaa
    14221 aaaaatattt acaataaaaa acagccgctt ttacgtatct tattagaaag agagacgaaa
    14281 ggcggtttta tgatgtatca aatagaagaa aatttattga aacttgctta tacaaaagga
    14341 atcagtagtc aaggaaaatg ggcacttgct aactggatga tgcagtatcc ttataaagaa
    14401 atcggatttg aagacatcat taagataggt aaaataacca aacatcggga gctgtttcgc
    14461 aaaagttggc aggaaattca cgctaactgg ggcaaaatcc aatcgaaaca gtcgtttatc
    14521 acttgctttg atcccaaata tccccctcag ctgcttcact tgacctatcc gccaattgta
    14581 ctgttttata atggtgatct atctttactt tcttgtaaca tgttaagcgt tgttggtgga
    14641 agacacactt ctgctttagc caaaaaaaca gtttattatt tactaaaacc agtgatagaa
    14701 gctggatatg tgatcgtcag tggctgcgcg aaagggatag atacatacgg tcaccaagca
    14761 gcaatctctc atggtggacg gacaatcgca gtcattggaa cagggataga gcaagtctat
    14821 cctgctgaaa atcgtgactt gcaagaggtg attgcaaatg atcatcttct tttgagtgaa
    14881 tatcctcctc aatccggccc gaaaaagcat tgttttccaa tgagaaatcg gattatcgca
    14941 gctttgtcta aaggaacatg tgtctttgaa gccaagaaaa aaagcggctc tctgataaca
    15001 gcacagcaag cattggatct tggaagaaca gttttttctg ttccaggaaa tattttaaca
    15061 gaacattcta ccggatgtca tcatttgatc caagacggtg cagtttgcac ggtttcgggt
    15121 gaagatatct tggcagaatt taaagaataa caccatactt ttcatttgct tttcttgaca
    15181 aacgttttct gaatggatat gattagctac gatttgtatg aatcctcttt taatagtagg
    15241 aacacgacaa tggaaaggag tccattcagt aatggcgtat aaatatttag tgattgtaga
    15301 atcacctgca aaagccaaga caattgagaa atatcttgga cgaaactata aagtaatggc
    15361 cagtgttggc catataagag atttaccgaa aagtaaaatg ggtatcgatt ttgaaaacaa
    15421 ttatgaaccc cattatattt ctatacgcgg aaaaggcgat gtcatcaaaa gcctgaaagc
    15481 cgcagcaaaa aaagctcaaa aagtttactt ggcaagtgac ccggatagag aaggagaagc
    15541 gattgcttgg catttagcgt acctacttgg gttggatctg aaagaaaaaa atcgggtggt
    15601 cttcaatgaa atcacaaaag acgcagtcaa agcagctttt aaggaaccaa gaacgatcga
    15661 tgtagattta gtagatgcac agcaagctcg tcgtacctta gacagaatcg ttggttattc
    15721 gatcagtcct attctctggc gtaaggtcaa gaaagggtta agtgcaggac gtgtccaatc
    15781 tgtcgcttta aaaattatta ttgaccgtga aaaagagatc cgagaatttg ttccagaaga
    15841 atattggagc atcgacggta attttaaaaa agctcgcaag aaattcaaag caaatttctg
    15901 gggaatcgac ggtaagaaaa agaaattacc agatgcacaa agtgtaaaag aagtcactgc
    15961 tagaattgat ggaacagact tcgaagttaa aaaagtagaa aagaaagagc gcaaaagaaa
    16021 ccctgcgaat cctttcacta caagtagcct gcaacaagaa gctgcccgta agttaaactt
    16081 tcgaacaaga aaaaccatga tggtagcaca gcaattatat gaaggaatca ctttgggtaa
    16141 acaaggaaca gtcggcttga ttacatacat gcgtactgat tctacccgga ttgccgatac
    16201 agcaaaagca gaggccgcag catttattga agatacgtat ggaaaagaat tttctagaca
    16261 tgatcaccga aagatcaaaa atgctcaagg cgcacaagat gcccatgaag ctgtgcgtcc
    16321 aacgagtgtg cttcgtacac cagatgaact caaacaatat ttagataaag atcaattgaa
    16381 actttataca ttgatctggt cacgttttgt ggctagtcag atgacaccag ctattttaga
    16441 tacaatgaaa gtgacattag aacaaaatgg tgtcatcttc attgcgaacg gttcaaaaat
    16501 caaattcaaa ggattcatgc aagtgtatgt ggaaggtaga gatgacggca aagaagagaa
    16561 agaaaatatt cttccagaac ttgaagaagg cgatgtagtc caatctgttg atattgaacc
    16621 aaaacaacat tttactcaac caccagcacg ttttagtgag gctacgttga ttcgtgcact
    16681 agaagaaaac ggggtagggc gaccttccac ttatgcacca acacttgata cgatccaaag
    16741 acgctactat gtaaaattga cacaaaaaag atttgagccg actgaactag gagaaattgt
    16801 caattcatta atctgtgaat tcttccctca aatcgtcgat atccatttca cagcagaaat
    16861 ggaaggagat ttggataaaa ttgaagaagg cacggaagcg tgggtcaaag tggttgatcg
    16921 tttctataaa ccgtttgaaa aagaactgac gaatgcggaa gaaaaaatcg aaaaaattca
    16981 aatcaaagat gaaccagcag gattcgattg tgatgtctgt ggacatccga tggtgataaa
    17041 attagggaaa tatgggaagt tttatgcttg cagtaatttt cctgattgtc ggaatacgaa
    17101 accaatcgta aaagaaatcg gtgtgacttg cccagtatgt catgaaggac aagtcattga
    17161 acgaaaatct aagaaaaaca gactattcta tggttgttcc cgtttcccag aatgtgaatt
    17221 cacttcttgg gataaaccag tgggcagaaa ttgtccaaaa tgtgacggat atctggtcga
    17281 gaagaaagtc aaaggcggta aacaagtagt ctgtgtaaat ggagattacg aagaagacat
    17341 ccaaaaataa tagaaaagag gttctttcat tgaaaatggt aaatgttgtt ggagcagggc
    17401 ttgccggaag tgaagctgct tggcagatcg cacaagcagg agtccctgtc cgtttatatg
    17461 aaatgcgtcc agtcaaatcg actgaggcgc atcacacaag taattttgct gaactagtat
    17521 gttccaattc attaagagga aacagtttgg ctaatgcagt agggttattg aaagaagaaa
    17581 tgcgccgatt gaattcggta gtaatcactt cggcagatga aacagcagtg ccagctggtg
    17641 gtgccttagc tgttgaccgc gattcttttt ctgaaacaat tacccgaaaa gtcaaagagc
    17701 atccattagt aacagtgata aacgaagaaa ttactgaaat accggaagga atcaccgtag
    17761 tagcaacagg acctttgact tctcatccat tagcagaaag catcaaagca ttcaatggtt
    17821 cagatggttt ttatttctat gatgcagctg caccaatcgt agacaaagct acgatcgata
    17881 tggataaagt atatttgaag tcccgctatg acaaaggaga agcagcttac ttgaactgtc
    17941 cgatgacaga agaggaattc aaagctttcc atgaggcact aatcaatgcc gaagtggcag
    18001 aacttaaatc ttttgagaaa gaaaaatatt ttgaaggatg tatgccaatt gaagtaatgg
    18061 cacagcgagg attcaaaacg atgcttttcg gacctatgaa accagttgga ttggaagatc
    18121 ctaaaactgg caaacgtcct tatgctgtta tccagctgcg tcaagacaat gctgcagctt
    18181 ctttatacaa catcgtgggc tttcagactc acttgaaatg gggcgagcaa aaacgtgttt
    18241 tccggatgat tcctgggtta gaaaatgctg aattcgttcg ctatggtgtg atgcaccgaa
    18301 acagctttat gaattcgcca gaattgctga aaccgactta ccaatcaacg aaacgcgaag
    18361 atctgttctt tgcaggtcag atgactggtg tagaaggcta tgttgaaagt gcagcgagcg
    18421 gactacttgc tggaatcaac gccgcacgct tagcaaaaga aatggaacca gtcgagtttc
    18481 cacaagaaac agctattgga agtatggctt actacattac tcatgcggaa ggaaaacatt
    18541 tccaaccgat gaatgccaac tatggtctgt ttcctgaact gccagagcgc attcgagaca
    18601 aaaaagcaag atatgaagca attgcacaac gagctttaaa agctaatgag gcaataaaaa
    18661 cagaattaga aaaaactgca atcccaaatg agtaaggaaa ctccatgaag agggtgagac
    18721 ggatagtctc gctcttttt
//
LOCUS       DACEEZ010000053        18247 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20313_2017
            SAMN12639966-rid9879223.denovo.053, whole genome shotgun sequence.
ACCESSION   DACEEZ010000053 DACEEZ010000000
VERSION     DACEEZ010000053.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639966
            Sequence Read Archive: SRR10023158
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 18247)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18247)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741683.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741683.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:44:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,016
            CDSs (total)                      :: 2,962
            Genes (coding)                    :: 2,889
            CDSs (with protein)               :: 2,889
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 36 of 73
            Pseudo Genes (incomplete)         :: 29 of 73
            Pseudo Genes (internal stop)      :: 18 of 73
            Pseudo Genes (multiple problems)  :: 8 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18247
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639966-rid9879223.denovo.053"
                     /isolate="E20313_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(1..633)
                     /locus_tag="IWO89_001848"
                     /pseudo
     CDS             complement(1..633)
                     /locus_tag="IWO89_001848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611665.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
     gene            1130..2752
                     /locus_tag="IWO89_001849"
     CDS             1130..2752
                     /locus_tag="IWO89_001849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0456580.1"
                     /translation="MNLKKIFSVKIFIVYAFVSLLYLFPAFKNMEIFGFYDTAFHINR
                     ALSLESIFSSPINFETFRSYGMQVNNFYPWLTLYPLFLLIKFTNLAIGYNLFLYIVTL
                     ITLFICHYVMYEITKKHVTSSFFAIIYTTSSFRSVEIFLRGAMGELLAMSILPLILLG
                     FIKLYDSKKESWVMLAISMTLLIYTHVLSVAMTMVMIIIALIIQFYRKKKIEIFLIIK
                     LIKAAIVTLLLSLAFIGPMIEQTLYQDLNRPYVDILQKRAIYLGKEFLIGSIDSELLS
                     FGIGLTLLICLIILTFNFKKLGILSKITYIMGIISILLCTKIFPWFALQNTPLNIIQA
                     PWRFMIFSTLFISFSVSLFISPQLEKFSYTRRRNFILLLILAISILNYSSVVNMVKEM
                     NFQGTYSNEQMENKLEEFENFDYVPIGYKKDQLLLKNHQVTEDGQVIYSSFSPNKDEI
                     TFTFKSSKGKTIVLPVFRYKGLTVTVNSKNATVLKQGGPFVTVKSESGINKVTIRYSY
                     TFFSKISLFISIITLIVLLFYCIKLKIRTSHE"
     gene            complement(3019..4188)
                     /locus_tag="IWO89_001850"
     CDS             complement(3019..4188)
                     /locus_tag="IWO89_001850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-lactamase family protein"
                     /protein_id="HAQ0456581.1"
                     /translation="MKKGKHAKKRKYSFLMIVVVLFSLSAFSTGTILGWQFYNNEHSQ
                     IVAHAQSAQKKLILTTNEQQLVEKIDQLLRKNDYIGSIYVKKNNRIIMEKGYGYANKL
                     TQQPNDPSLYYQIGSVQKALTTLLILKQVERGHLSLDTKLAQFYPQIAGSQQITIRDL
                     LYMRSGLHRTASPTIPMTDEEVVQFAIQHLKFTDYQTSHYDSLNFTILTGILIKLTNQ
                     PYEKLLTDDIIEPLGLQHTKFYDQVKNSSNHALSYQMSKEDDYFKPLTESETEIHNEL
                     GTGNISMSVYDLNTLFTQVLSGKLVSKDLLFSLWRENANGHPYSGGVYSGDNYILSQG
                     NIDSFDTSAIFSKDSQDAIIMESNIKADKTIKLPTADLRNQIYDLMEGTTKLANS"
     gene            4392..5567
                     /gene="mltG"
                     /locus_tag="IWO89_001851"
     CDS             4392..5567
                     /gene="mltG"
                     /locus_tag="IWO89_001851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic transglycosylase MltG"
                     /protein_id="HAQ0456582.1"
                     /translation="MSGNKQHPSKDKKEASKAATADIIDKRRNLRKKEDKIVRKIILV
                     IALTLLIIGGFLGFTVYRYVDSGLKPLDKSDDQLVQVEIPSGSSNKQIGEILEKDNII
                     KSGIVFNYYTKFKNLTGFQAGYYQLAPNMTLDEIGKQLQEGGTSEPTKVADGKIAIPE
                     GYDIDQIAERVAKVTGKDKKEFLDLVNDETFFNELHQKFPELLESASKAENTKYRLEG
                     YLFPATYDYYTSTSLKDLVIEMVNKTNTVMQNYYSAIKQKNLTVQEVLTLASLVEKEG
                     VKENDRKNIAQVFFNRIKANMPLQSDISVLYALGEHKELVTYEDTAVDSPYNLYTNTG
                     YGPGPFDNPSEEAIKAVLEPAENDYYYFVADTSTGNVYFAKTYEEHMELVEKYVNNS"
     gene            5703..6182
                     /gene="greA"
                     /locus_tag="IWO89_001852"
     CDS             5703..6182
                     /gene="greA"
                     /locus_tag="IWO89_001852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016172390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HAQ0456583.1"
                     /translation="MVEKVYPMTLEGKEKLEKELEELKTVKRGEIIERIKIARGFGDL
                     SENSEYESAKDEQAFVEGRITTIENMIRFAQIIDNDGVDADEVSIGKTVTFIELPDGE
                     EEEYTIVGSAEADPFSGKISNDSPIAQALIGKRKNDEVTIATPGGDMLVKIVKVEQA"
     gene            6351..7082
                     /locus_tag="IWO89_001853"
     CDS             6351..7082
                     /locus_tag="IWO89_001853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-active antibiotics response protein"
                     /protein_id="HAQ0456584.1"
                     /translation="MNSSWRFFVVVEALLLLFAVWQIVNNTGLLLLVIFGIFNIYLAM
                     RKHPRTKFQNFQLILGSLVIFFSLVNSPALWMMAVFAVLFIGLKGVEISGIDLTKNAF
                     WRKKQIMMIQTEQLKTHNNERKRQQLFGNQRIGNDVYEWDDINIAVISGDTIIDLGNT
                     LLPKDDNIVIVRKGIGRTRILVPLGVAIRLEHATLVGNVLFEEEQFSLKNEQIKIYSS
                     DYDENPRRLKIITNTLIGDVEVIRI"
     gene            7079..8146
                     /locus_tag="IWO89_001854"
     CDS             7079..8146
                     /locus_tag="IWO89_001854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HAQ0456585.1"
                     /translation="MMGKISRAMLAVYSGIAAFLIILFSLFTYFYASNQSHWWGELLR
                     ARLLYVPLIFHLLAISLGVGLIVFLLLSLIQKTKYGKIEEKLRALSSGNYESKLLLLP
                     IPSASDDLYIKDIDKEITKIKEKMIEVSSELQIVTSRPQYVDGQTKEEILELERHRLA
                     RELHDSVSQQLFAAMMMMSALTEQAEKSETPEMFRKQLKMVAEIINASQSEMRALLLH
                     LRPVNLEEKSLKQGIEQLLKELQNKIQISLKWDVEDVKLSSSIEDHLFRIVQELLSNT
                     LRHAKANELEVYLHKIDNNLLLRIIDDGTGFNMNETKTGSYGLNNIKERVAGIGGTVK
                     IISFKGQGTSVEIKVPLMKEA"
     gene            8153..8785
                     /locus_tag="IWO89_001855"
     CDS             8153..8785
                     /locus_tag="IWO89_001855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAQ0456586.1"
                     /translation="MIKVLLVDDHEMVRLGVSSYLSIQEDIEVIGEAENGRQGYEKAM
                     TLRPDVILMDLVMEEMDGIESTKAILKDWPEAKIIIVTSFIDDEKVYPAIEAGAAGYL
                     LKTSTAHEIADAIRATQRGERVLEPEVTTKMMEKMSRRNEPVLHEELTNRENEILMLI
                     SEGKSNQEIADELFITLKTVKTHVSNILAKLEVEDRTQAAIYAFKHGLVK"
     gene            8925..9590
                     /locus_tag="IWO89_001856"
     CDS             8925..9590
                     /locus_tag="IWO89_001856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrkA family potassium uptake protein"
                     /protein_id="HAQ0456587.1"
                     /translation="MKQNFAIVGLGRFGGSICRTLIEAGQEVLAIDSNEDRVNEYMNI
                     ATHAVVANAQDEMTLRSLGIRNFDHVIVAIGEDIQASILVTLMVKEMGVPNILAKAQN
                     EYHARVLEKIGADRVVHPERDMGIRIAHNLVSKNILDYLELSDKYSLAEIRVSNPKFF
                     NKTLAELNFRQRFDLTVVAIRRVDQTVIASPAADEYVRENDNLLVIGETGDVDILDDK
                     MNQ"
     gene            9618..9911
                     /locus_tag="IWO89_001857"
     CDS             9618..9911
                     /locus_tag="IWO89_001857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster biosynthesis protein"
                     /protein_id="HAQ0456588.1"
                     /translation="MKLTVTPKAQKWFDSEIDLPETYGIRFFGKVYGKTEVHDGFSIG
                     MSVEQPEHPVKKVEMDGTLFFIEEADEWFFKGYDLLVDYDPNLEEPTYHFKKQ"
     gene            complement(9979..10926)
                     /locus_tag="IWO89_001858"
     CDS             complement(9979..10926)
                     /locus_tag="IWO89_001858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyacid dehydrogenase"
                     /protein_id="HAQ0456589.1"
                     /translation="MSERIIYADRPFRDEFIEEIHTIAPGYIFKTELQPEDLQNVEIS
                     LGWNSDYQEKLLASSTLKWVQSISAGVDQLPLETFSKKHILLSNGSGIHSESITEHVL
                     GIILGYSRGLFQAQKAQSDMKWLGSSVHYQSIQGKKILIIGTGHIGKMIAKKCKALDM
                     NCYGINTSGHPAEGMEKTFSLNQLKEVLPEVDFVVNILPLTEETTGLFDQHMFEHFDP
                     KSVFINVGRGASVKTQDLVQALNNQQLSFAALDVFEEEPLPQDHPLWKMDNVLITSHI
                     AGLTPDFQKKLMAIFLTNLKSYFATHELQTNQVKLSAGY"
     gene            complement(11104..11907)
                     /locus_tag="IWO89_001859"
     CDS             complement(11104..11907)
                     /locus_tag="IWO89_001859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1189 domain-containing protein"
                     /protein_id="HAQ0456590.1"
                     /translation="MTTKQLIISSFIRFKDLKEARNVPFWKAIVYLLVLSIIMALPIS
                     FQVFQVLNNIQLDGQKIATRIPDFTIQNGQLQTEEKEGFIYQTNSIIFTFDPEGKRTE
                     QDISTDLMGNFVSVGMLKNKLIIALPNTGTTSALLNNNQLELPYTNESLKNLTGKQLR
                     SFLSEASIPLWIKVLTFIFSIYPSFLNLLITLLFTNIAAFLYARFRLTKATFLDCLKT
                     LIYSISLPTIIATILMVFLPSFDTSAFIAFAGIFIFAQAVKGWAKISIN"
     gene            12053..12967
                     /locus_tag="IWO89_001860"
     CDS             12053..12967
                     /locus_tag="IWO89_001860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation transporter"
                     /protein_id="HAQ0456591.1"
                     /translation="MEEGIVPTQPQRSSKKLFGVVVLNAVITLSEFIGGIMSGSLSLV
                     SDAFHNLSDTLAIIFSYGAQKMARKEANKKRTYGYQRLEILSAFINSFILIILSLFLT
                     VEAFKRFTSPEKINSHLMLTVAVIGLLANLFSVLLLRQEADESLNIKSSYLHLLSDTL
                     SSVSVIIGAILIRFFDIYWIDPVITLIISIYILIEAIMVIKKAAAILIQSAPAIDYEK
                     MEQEIEAIDGVKDVHHVHIWQYSEKIIIFDGHIDFEDQLLSEIEKIYPRIISLLKLKY
                     GITHVTIQAETKAKDQKKLIFLNKDESY"
     gene            13015..13485
                     /locus_tag="IWO89_001861"
     CDS             13015..13485
                     /locus_tag="IWO89_001861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GtrA family protein"
                     /protein_id="HAQ0456592.1"
                     /translation="MKQYRQLKGYLEAKGYWEILVYLFFGGLATIVNFVSFALARQYF
                     DLSMALSNSISWFCSVLFAFVTNKLWVFHSKSPNFTHALIECGKFFFYRILSYGLDMG
                     AMVLLINVMNSNEYVAKIITQIIVILANYIFSKLFIFKETEVFEEEVGQSEKDD"
     gene            13570..14178
                     /locus_tag="IWO89_001862"
     CDS             13570..14178
                     /locus_tag="IWO89_001862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase"
                     /protein_id="HAQ0456593.1"
                     /translation="MTYTLPDLPYAYDALEPYIDEETMHLHHDKHHNTYVTNLNSAIE
                     KYPELGEKTIEELLSDMDAIPTDIKTAVRNNGGGHANHSFFWEIMAPNAGGEPTGEIK
                     EAINEAFGDFSSFKEEFKKAAAGRFGSGWAWLVMENGKLAITSTANQDSPLMEGKTPI
                     LGLDVWEHAYYLKYKNVRPDYIAAFWNVINWDEVNKRFEAAK"
     gene            14312..14512
                     /locus_tag="IWO89_001863"
     CDS             14312..14512
                     /locus_tag="IWO89_001863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HAQ0456594.1"
                     /translation="MEHGTVKWFNNEKGFGFITVDGGDDVFVHFSAIQGDGFKSLEEG
                     QEVEFTIVEGARGPQAAEVTKL"
     gene            15266..16492
                     /locus_tag="IWO89_001864"
     CDS             15266..16492
                     /locus_tag="IWO89_001864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LytR family transcriptional regulator"
                     /protein_id="HAQ0456595.1"
                     /translation="MKRWQKVLLTLLGVFVLLIAGLSAYGIRFMGEASQTVNKISKNS
                     NRISSKRSERVSIDDKEPFSVLLLGLDTGGLGRTEQGRSDTMMVVTVNPKQKKSTIIS
                     LDRDIYTNIVGYGTVDKLNHAYAFGGVEMAMDSIEQLLDIPIDHYVTINLDGMEDLIN
                     AVGGIKVNNKIDFTLDGVHVPVGEQTLNGEKGLAYSRMRHEDPEGDIGRQRRQREVVT
                     KIVNKVLSLDGVSNYRKILKAVENNVTTDLDWDDMLDVATNYTPAFETIKQDQLQGEG
                     QMINDIYYQILGKNDLLSIQNELKKQLNIKTSDTLPNLKNENASMMFFDDSEDGDSTN
                     GDGSNSEGYTDYSSYSENTYDQYQDPNTYYQQTEDTSQYYTPSQDTQSYDPYAGQEQN
                     GYGYDQNNEYGTTSGY"
     gene            complement(16600..17070)
                     /locus_tag="IWO89_001865"
     CDS             complement(16600..17070)
                     /locus_tag="IWO89_001865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan-rich sensory protein"
                     /protein_id="HAQ0456596.1"
                     /translation="MKMIKDIRFWVCVIGIVILGFLSGLLSGNPGKYYYSLQLPPFAP
                     PSWIFGPMWTLLYILMGISLYLLLNHNNKKQRNNLVGLFVIQFIFNFIWSALFFNLRN
                     IFIAAIDITLLVIFLSVLMYQLWLHHRLTMWLMIPYYLWVLFATLLNYSIYFLN"
     gene            17392..17970
                     /locus_tag="IWO89_001866"
     CDS             17392..17970
                     /locus_tag="IWO89_001866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chitin-binding protein"
                     /protein_id="HAQ0456597.1"
                     /translation="MKKISTLLFIGLVSLGLFGFSIPGFAHGYITSPGSRAYLGTSAA
                     GNLNQNVGRAQWEPQSIEATKNTFIDGKIASAGVSGFEPLDEQTSNRWHKNMVNPGTL
                     NITWNLTAQHRTSTWDYYITKPTWNPNQPLKFSDFELITKIDDKATVPPKTVNQTITL
                     PQDRKGYNVILAVWNISDTTNAFYQVIDVNIQ"
BASE COUNT         6196 a         2802 c         3486 g         5763 t
ORIGIN      
        1 ggtaaaaaat aagtctgatt ccttgtctct aaaggcttcg gtcagctcat ggaaaaaagt
       61 ataggcgtcc tttaaaggcg ttgaaaatgc cagcaatcga tcaatcatca ttgcttcagt
      121 gagtagcggg tatttaggtg cacgaaaact gcgccatgtt ttgtaatcat agtgattgat
      181 tttttgacgg ttttttagaa gaaagcgcca gttctttttc actttctcgg cttcccgtcg
      241 ttgccctaac tggtgtaatc ttttcatttc ttgtacacga aaatcattga acgcctgatt
      301 caaatgtttg atgatatgaa aacaatcaat gacgactttc gcatttggta agacacgttt
      361 tgtcagttgg aagtaggcgg cgttcatatc tgtcactaaa tattccaccg cttcaggatt
      421 tgcacagcga ctaaaatagt gtgttaatcg agacaatttt cgtgtaggca aaatatcaat
      481 gagttttcca gtctcgccat ccgcacaaat aaagctcatt ttatccacaa ttgaagcgtg
      541 cgaacgaaat tcatccacca tcaatacttt tggcagggtc ctttttgacg gcttcggaag
      601 atagtttttc aactctttca atgtacgaat catataccta aaataacaat gggactgaag
      661 aatcaatttt gattcatcag tcccataaat tatagagcct atattttgcg aattgcgcta
      721 cggtttcttc ttttcttttg tctgtaatat gaatatatag atccataatg atttgaatag
      781 aagagtggcc taaacgctct tgcacatctt taatatttgc actagcttgc taaaagcaag
      841 ctagcatgtg tgtgtctaag accatgaata gtaattcgtt taagattgtt tttcctgatg
      901 attacatcta accattttcg agatttagat aattgaaggt gttcatttct ttctggtaaa
      961 aatttaaata tataactaca ttttctctac taactttgaa acactacttt tgtaatataa
     1021 aaaatatttt attaataata aaattaatca cattttatat agtgagtaat ctagtgtttt
     1081 aaaaaaataa atgatactat atgtaatata aaaaaacatg aggtggtata tgaatttgaa
     1141 aaaaattttt tctgtaaaaa tatttatagt ttatgcattt gttagtttat tatatttatt
     1201 tccagcattt aaaaacatgg aaatatttgg cttttatgat acggcatttc acattaatcg
     1261 tgcgttaagt ttagaatcta tttttagctc ccctattaat tttgaaacgt ttagatcata
     1321 tggcatgcaa gtaaataatt tttacccatg gttaactcta tatcctttat ttttattgat
     1381 caagtttact aatttagcaa taggctataa cctttttctt tatattgtta cgctcattac
     1441 tctatttata tgtcattatg taatgtatga aattaccaaa aaacatgtta ctagctcctt
     1501 tttcgctatt atatatacaa catcttcttt taggagtgta gaaatatttc ttcgaggggc
     1561 aatgggggaa ttactcgcaa tgtcaatact tcctcttatt ttactcgggt ttataaagct
     1621 ttacgactca aaaaaagaat cttgggttat gttggcgatt agcatgactt tattaattta
     1681 cactcatgtt ctttctgtag ctatgactat ggtaatgatt ataattgctt taattattca
     1741 attttatcgg aaaaagaaaa tagaaatctt cttaattatt aaattaataa aagctgctat
     1801 agttacttta cttctttcac tagcgtttat aggtcctatg attgagcaga cattgtatca
     1861 agatctgaat agaccttatg tggatatatt acaaaaaaga gcaatttatc tgggtaaaga
     1921 gtttttgatt ggaagtatag attcagaatt actatcattt ggtattggct taactttgct
     1981 tatttgttta attatactaa catttaactt taaaaaatta ggaatcttat ctaaaatcac
     2041 ttatataatg ggtataatat ctattctact ttgtacaaaa atatttccat ggtttgcctt
     2101 acaaaatact cctttaaata ttattcaagc tccttggcgt tttatgatat tttcaacatt
     2161 gttcatatct ttttcagtat ctctatttat ttctccacag ttggaaaaat tttcttatac
     2221 aaggagaaga aattttatac tattattaat attagctatt tcaattttaa attattcatc
     2281 agtagttaac atggtgaaag aaatgaattt tcaaggaact tattctaatg aacaaatgga
     2341 aaataaactt gaagaatttg agaattttga ttatgtacct ataggctata aaaaagatca
     2401 attgcttttg aaaaaccatc aagttacaga agatggtcaa gttatttata gtagcttttc
     2461 tccaaataaa gatgaaatta catttacgtt taaatcttca aaaggcaaaa caatagtttt
     2521 acctgtattc cggtataaag gattaacggt tactgttaat tcaaaaaatg ctacagtact
     2581 aaaacaagga ggtccttttg ttacagtaaa aagtgaaagt ggaataaata aagtaacaat
     2641 caggtattca tacacatttt tttctaaaat ctcattattt atttctataa tcacgctaat
     2701 tgttcttctt ttttattgca taaaattaaa aataagaaca agccatgaat gatttgctag
     2761 cacatatttt agcaatctaa agatactatt ttagatttaa taggcccttt gtcaaataat
     2821 gttggtaaag agaaatctta gaataatttg gacagaacag aaagaagaac acctctttct
     2881 gttctgttta tttggaatta aagtctgtag caaaaattga tttttagatt tgtcccacac
     2941 tctttcctac gtttagtcga gtactttttt taagcaaatg ctattcaaag aagtagtatt
     3001 tgctatcttt tcatcatatc agctatttgc caacttagta gttccttcca tcaggtcata
     3061 gatttggttt cttagatcag cagtaggcag tttgatagtt ttgtcagctt ttatattgct
     3121 ttccatgatg atagcatcct gtgaatcttt gctaaatata gcagatgtat caaaagagtc
     3181 gatatttcct tgcgacagga tataattatc tccgctatat accccaccac tatatggatg
     3241 tccattcgca ttttcacgcc ataatgagaa gagtaagtct ttggaaacta gttttccaga
     3301 taaaacttgt gtaaacagcg tatttaaatc atagacggac atactgatat taccagtccc
     3361 taattcgtta tggatctctg tttcagattc tgtcagtggt ttaaagtaat catcctcttt
     3421 agacatttga taggatagtg catgattgga tgaatttttg acttggtcat aaaattttgt
     3481 atgctgtaat cctaaaggtt cgatgatatc atcggtcaat aatttttcat acggttgatt
     3541 tgttaatttg atcaaaattc cagtcaatat ggtaaagttc aatgaatcat aatgagaggt
     3601 ctgataatct gtaaatttta agtgctgaat cgcaaattga acgacttctt catcagtcat
     3661 cggtatggtt ggagaagcag ttcggtgcaa gcctgagcgc atatacagca agtcacggat
     3721 agtaatttgc tggctgccag caatttgtgg atagaattga gccagcttag tatctaaaga
     3781 aaggtgccca cgctcgactt gttttaaaat caaaagagta gtcaatgctt tttgaaccga
     3841 accaatctga taataaagag aaggatcatt tggttgttgc gtgagtttat tcgcatagcc
     3901 atagcctttt tccatgatga tgcgattatt tttctttacg taaattgagc caatataatc
     3961 attttttctt aatagttgat cgattttttc aacgagctgt tgttcattgg tagtcagtat
     4021 caactttttt tgagctgatt gagcatgggc aactatctga gagtgttcat tattgtaaaa
     4081 ttgccatcca agaatagttc ctgtagagaa cgcactaagg ctaaacaaca cgacaacgat
     4141 cattaaaaat gaatattttc tttttttagc atgttttcct tttttcataa tagtttattt
     4201 agtctccttt cttttttaca gaaagaataa agctaaaata gcttgtatct tgataacatt
     4261 tattttaatc ttatagtaat ggatttacta cttacatttt tgatagaata tttaaataaa
     4321 atcattcacg ccaattcaaa tatcatgtat aatgaataaa gttacgaact tgatagtaag
     4381 gaggagttgt tttgtcagga aacaaacaac acccaagtaa agataaaaaa gaagccagta
     4441 aggcagctac agcagatata atcgacaagc ggagaaatct gcgtaaaaaa gaagataaga
     4501 ttgtgaggaa gatcatttta gtgattgctc ttacattatt gattattggc ggatttcttg
     4561 gatttaccgt gtatcgttat gtagatagcg ggttgaagcc tttagataaa tcagatgacc
     4621 aattagtcca agtcgagatc ccgagtggct cttctaacaa gcagattggt gagattttgg
     4681 agaaggacaa tattattaaa agcgggattg ttttcaatta ttatacaaaa tttaaaaatt
     4741 taactggctt tcaagctggc tattatcagt tagcgcctaa tatgactctt gacgagattg
     4801 gcaaacagtt gcaagaaggc ggtacttctg agccaacaaa agttgcagat gggaaaattg
     4861 ccattccaga agggtacgat atcgatcaga ttgctgaacg ggtagcgaag gtaactggta
     4921 aagacaagaa agaattttta gatttagtga atgatgagac tttctttaac gagttgcacc
     4981 aaaaattccc agaactgttg gaaagtgcat ctaaagctga gaacacaaaa taccgtttgg
     5041 aaggttactt attcccagcc acatatgatt attacacgag tacttcgctt aaggatctag
     5101 tgattgaaat ggtgaacaag acgaatacgg tcatgcaaaa ctattattca gctatcaagc
     5161 agaaaaattt gactgtacaa gaagttttaa ctctagcttc tctagttgaa aaagaaggcg
     5221 taaaagaaaa tgatcgtaaa aacattgcac aagtcttctt taaccgaatc aaagcaaata
     5281 tgccacttca gtcagatatc tctgttttat acgcattagg tgaacacaaa gaattagtga
     5341 cgtatgaaga tacagctgta gattcaccat ataatttgta taccaatacc ggctatggac
     5401 ctggaccttt tgacaatcca agcgaagagg caatcaaggc agtattagaa ccagcagaaa
     5461 atgactatta ttatttcgta gcagatacga gcactggaaa tgtttatttc gctaaaactt
     5521 atgaagaaca tatggaatta gtggaaaaat atgtaaataa ttcttagaaa aacaggctct
     5581 agacattgat catttttaga aaaacattta catttgaaga aaagcgtggt aatctttttt
     5641 ggactatatt cctcaaaaga ttaccttttg cggtatttta tcgaaaaaag gagacgttac
     5701 acatggtaga gaaagtttat ccaatgactc tagaaggtaa agaaaaatta gaaaaagaat
     5761 tagaagaact aaaaacagtc aaacgcggag aaatcattga acgaatcaaa attgcccgcg
     5821 gtttcggaga tctttccgaa aactctgaat atgaatcagc gaaagatgaa caagcatttg
     5881 ttgaaggaag aatcacaaca attgaaaata tgattcgttt tgctcaaatc atcgataacg
     5941 acggtgtaga tgcagatgag gtatcaattg gtaaaacagt tacattcatc gaactaccag
     6001 atggagaaga agaagagtac acgattgtcg gaagcgctga agcagatccg ttttctggca
     6061 aaatttccaa tgattctcct attgcgcaag cattgattgg caaacgtaaa aatgatgaag
     6121 taactattgc tactccaggt ggcgatatgt tagttaaaat cgtcaaagta gaacaagcgt
     6181 agtctgactg aaagatctag agagttcatc ttactgatga tacactctag atctttttct
     6241 ctttgtgtat tttagatttc aacaaacgat cttacatcta aagtcctata gccaacagtt
     6301 aaaaaaatag gtatgatgaa atgagaatgt gtcaggaggg agaaagagcg atgaatagtt
     6361 cttggcgttt ttttgtggta gttgaagcac tactgctgtt atttgctgtt tggcaaatcg
     6421 taaacaatac tggacttttg cttttagtga tattcggtat tttcaatata tatctagcta
     6481 tgcgcaaaca tcctcgtact aaatttcaaa attttcaatt gatattagga agtttggtca
     6541 ttttttttag cttagtcaac agtcctgctt tatggatgat ggctgtattt gccgttttat
     6601 ttattggtct gaaaggtgta gagatttcag ggatagattt aaccaaaaat gctttttggc
     6661 gaaagaaaca gatcatgatg atccaaacag agcaattaaa aacacataat aatgagcgga
     6721 aaagacagca attattcggt aaccaacgta ttggaaacga cgtctatgaa tgggacgata
     6781 tcaacatcgc cgtcatttct ggtgatacta tcatagattt gggcaatacg cttttgccaa
     6841 aagatgataa tattgtgatt gtaagaaaag ggataggcag aacacggatc ttagtacctt
     6901 tgggcgtagc catccgatta gagcatgcga cattagtagg aaatgtcttg tttgaagaag
     6961 agcaattttc tttgaaaaat gaacaaatca aaatctacag cagtgattat gatgaaaacc
     7021 ctcgtagatt gaagattatt actaatacct tgattggaga tgtcgaggtg atacgaatat
     7081 gatgggaaaa atatccagag cgatgctagc tgtttattcg gggattgcgg ctttccttat
     7141 tattctattt tcacttttca cttattttta tgccagcaat caaagtcatt ggtggggaga
     7201 attactgcgt gcacgtttat tatatgttcc gcttatcttc catttgttag ccatttcctt
     7261 aggtgtagga ttaattgtct ttctattatt atcacttatt caaaagacaa aatacgggaa
     7321 aatcgaagaa aagctgcgtg cactttcttc tggcaattat gaatccaaat tgttgcttct
     7381 tccgatccca agtgcatcgg acgatttata catcaaagat attgataagg aaatcactaa
     7441 gataaaagaa aaaatgattg aagtatctag tgaattacaa attgtaacga gccgtcccca
     7501 atatgtagat ggacaaacca aagaagaaat tttagaatta gaaagacatc gattagcccg
     7561 tgagctgcat gattcagttt cgcaacaatt gtttgcagca atgatgatga tgtcggcatt
     7621 gacggagcaa gcagaaaaaa gcgagacacc agaaatgttt cgcaagcagt tgaaaatggt
     7681 agcagagatc atcaacgctt cccagtctga gatgcgtgcg ctgcttcttc atctacgtcc
     7741 agtcaattta gaagagaaaa gtttaaagca aggtatcgag cagttattga aggaattgca
     7801 aaataaaatc cagatttcgc tgaaatggga tgtagaagat gtaaaactat ctagttccat
     7861 tgaggatcac ttgttccgaa tcgttcaaga attgttatca aatacattaa gacatgctaa
     7921 agccaatgaa ttagaggtat atttgcacaa aatagacaat aatcttttgt tacgtattat
     7981 tgacgacgga acagggttta atatgaatga aacgaaaacg ggaagttatg gattgaacaa
     8041 tatcaaagaa cgagtagctg gtattggtgg aacagtaaaa atcattagtt ttaaaggtca
     8101 aggtacaagt gtagaaatca aggtcccttt gatgaaggag gcatagataa ggatgataaa
     8161 agttttatta gtagatgacc atgaaatggt gcgcttaggc gtctcttctt atttatctat
     8221 tcaagaagac attgaagtaa ttggagaagc tgaaaatgga cgacaaggct atgagaaggc
     8281 gatgacactt cgaccggacg tcattttaat ggacttagtc atggaagaaa tggatggtat
     8341 cgaatcaaca aaagcaatct taaaagattg gccagaagct aaaatcatta tcgtaaccag
     8401 ttttattgat gacgaaaaag tttatcctgc cattgaagca ggtgcagcag ggtatttgtt
     8461 gaagacctct accgcacatg aaattgctga tgctataagg gcaacgcaac gtggcgagcg
     8521 agtattagaa ccagaagtga caacaaaaat gatggaaaaa atgagtcggc gaaatgagcc
     8581 tgtattacat gaagaactaa cgaatcggga aaatgaaatt ttaatgctga tttctgaagg
     8641 aaaaagtaat caggaaatag cggacgaatt gtttattaca ctgaaaacag taaaaacaca
     8701 tgtttccaat atattggcta agctagaagt ggaagaccgt acacaagccg ctatctatgc
     8761 attcaaacat ggtttggtga aataaaaacg atgataaaga ggataatcaa aagctgagaa
     8821 aataactatt atttttcttg gcttttttac aataatgatg cttgcttttg tttttgtaaa
     8881 ggctttgtta tactgagaaa agaagatact aaaggaggaa aacggtgaaa caaaattttg
     8941 caattgtagg tcttggacga tttggcggaa gtatctgccg aacactgatc gaagctggtc
     9001 aagaagtact ggctattgat agtaatgaag atcgggtcaa tgaatatatg aatatagcca
     9061 ctcatgctgt tgtggcaaat gcacaggatg aaatgacact tcgatcatta ggcataagaa
     9121 atttcgatca tgtaattgtc gctattggag aagatattca agccagtatc ttagttactc
     9181 tgatggtaaa ggaaatgggc gtaccaaata ttttagcaaa agcccaaaat gagtatcatg
     9241 ccagagtact tgaaaagatc ggagctgatc gcgtcgtcca tcccgagcga gacatgggaa
     9301 taaggattgc gcataattta gtgtcaaaga atattttaga ttacctagag ttgtcagata
     9361 aatattcttt agcagaaatc cgagtctcca atcctaaatt tttcaacaaa acattagctg
     9421 aattaaattt caggcaacgt tttgacttaa cagtagtcgc catacgaaga gtagatcaaa
     9481 cggttatcgc ttctcctgca gcagatgaat atgttcgtga aaatgataat ctactggtta
     9541 ttggagagac aggcgatgtg gatattctag acgataagat gaatcaatag aaagaaaaga
     9601 gaaagtaggt tgttgggatg aaattaacgg taacgccaaa agcacaaaag tggtttgact
     9661 cagaaattga tctgccagaa acttacggaa tacgtttttt tggaaaagtc tacggcaaaa
     9721 cagaggttca tgatggtttt tcgattggaa tgtcagttga acagccagaa catcctgtaa
     9781 agaaagtgga aatggatggg acactttttt ttatcgagga agcagatgaa tggttcttta
     9841 aaggatatga tttacttgtt gattatgatc ccaacctgga ggagccaacc tatcacttta
     9901 aaaaacaata aaaaaagaga gggtgacaca atttttgtca ccttctcttt tttcttttga
     9961 aaatattgtt ttcagctatt aatatcctgc agacaatttt acttgattgg tttgaagctc
    10021 atgggtggca aagtagcttt tcagattggt taaaaaaata gccattaatt tcttttgaaa
    10081 atcaggtgtc aaacctgcaa tgtgggaagt aatcagtaca ttgtccattt tccaaagagg
    10141 atgatcttgt ggaagaggct cttcctcaaa aacgtctaat gctgcaaagg aaagttgctg
    10201 gttatttaaa gcctgcacca gatcttgtgt cttaacagaa gcaccacggc caacattgat
    10261 gaatacagat tttggatcaa agtgttcaaa catatgctga tcaaagagcc cagttgtttc
    10321 ctccgttaga ggtaagatat tgactacgaa atctacttca ggaagaactt cctttagttg
    10381 atttaaagaa aaagtctttt ccattccttc tgctggatga ccggatgtat tgattccata
    10441 gcagttcatg tctaatgctt tgcatttttt tgcaatcatt tttccaatat ggccagttcc
    10501 aatgattagt atttttttcc cttggataga ttggtaatgg accgatgaac ctaaccattt
    10561 catgtcactc tgtgcctttt gtgcttgaaa aagaccacga gaatacccaa ggatgattcc
    10621 taagacatgc tccgtaatcg attcactgtg gatgccacta ccatttgaaa gtaaaatatg
    10681 tttctttgaa aaagtctcaa ggggtagctg atcaacacca gctgagatag attggaccca
    10741 tttcaaggta gaagaggcta atagtttttc ttggtagtct gagttccagc ccaaactgat
    10801 ctctacattt tgtagatcct ctggttgtaa ttctgtttta aagatataac caggcgctat
    10861 agtatggatt tcttcgataa attcatcacg aaatggacga tctgcataaa tgattctttc
    10921 tgacatagtt aatttcccgc ctttctttac tctaagtgta gcgcatacaa gaagaacttt
    10981 gaagcgcctt gcttgttttt tgagaaagaa atatttttca aataaaaaag gggctgtgac
    11041 aaaaaaatgt cacaacctct aaaagggtct attgaagtag aaataacgag ttgtctttat
    11101 ttcttaattg atcgatattt ttgcccatcc tttgacagct tgagcaaaaa taaagattcc
    11161 tgcaaaagca atgaaggcgc tagtgtcaaa agaaggaaga aagaccatca agattgttgc
    11221 aattatagta ggcaatgaaa ttgaatagat caatgttttt aaacaatcaa gaaaagtagc
    11281 ttttgttagt cgaaagcgtg catagagaaa agcagcaatg ttggtaaata gcaaagtgat
    11341 cagcagattc agaaaactag gataaataga aaatataaat gtcaggactt ttatccaaag
    11401 tggtatagat gcttcactta aaaaagaacg gagctgctta cctgttaagt tttttaaaga
    11461 ttcattggtg tagggaagtt ctaattggtt attgttcaac aaagcagaag tcgtgcctgt
    11521 attaggaaga gctatgataa gtttgttttt taacatcccg acactaacaa aattccccat
    11581 caaatcagta gagatgtcct gttctgttcg ttttccctct ggatcaaatg tgaagataat
    11641 ggagtttgtt tggtaaatga atccttcttt ttcttcggtt tgaagctgcc cgttttggat
    11701 tgtaaaatca ggaatgcgag tagcaatctt ttgcccatcc aattgaatgt tgtttaagac
    11761 ttgaaaaact tgaaaagaga ttggcaaagc cataattata ctcaaaacta aaagatagac
    11821 aatggctttc caaaatggga catttctcgc ctcctttagg tctttgaatc gtataaagga
    11881 actgataatt aattgttttg ttgtcataag tactcctcat ctagtagtgt catcacttgc
    11941 aattctagcg aagtatcaag tgaaatacaa gtgctttaag gaaacagctg aatgattggt
    12001 gaaatatcaa aaaaatgtta ttcttgtcta aagactatcc aatataaata aaatggagga
    12061 aggaatagtg cctacacagc cacagagatc atcaaaaaaa ctatttggag tagtggtttt
    12121 gaatgcggtg atcactttat cagaatttat tggagggatt atgtcaggga gtttgtctct
    12181 tgtttcagat gctttccata accttagtga tacattggca attatttttt cttacggggc
    12241 acaaaaaatg gcccgaaaag aagcaaataa aaaacgtacg tatggctacc agcgtttaga
    12301 aatattgagt gctttcatca actcattcat attgatcata ttatccttgt ttttaaccgt
    12361 tgaagcattc aagcggttca cttcaccaga aaagataaat agtcatttga tgctaacagt
    12421 agcagttatt ggattgctcg cgaatttgtt ctcagtatta ttattacgac aagaagcaga
    12481 tgaaagtttg aatataaaat ctagttattt gcatttgtta agtgatacac tgtcttcggt
    12541 aagtgtgatt atcggagcaa ttttgatccg ttttttcgat atctactgga tcgatccagt
    12601 tattacactg atcatttcga tatatatatt gatagaagcg ataatggtga tcaaaaaagc
    12661 tgccgctatt ttgatacaaa gtgcacctgc tatcgactat gaaaaaatgg aacaagaaat
    12721 tgaagcaatc gatggggtaa aggatgttca tcatgtccat atttggcagt atagcgaaaa
    12781 gataattatt ttcgacggac atattgattt tgaagatcag ctactaagcg agattgaaaa
    12841 gatatatcct cgtataattt ctttattaaa attaaaatat ggtattacac atgtgacgat
    12901 ccaagcagag acaaaggcaa aggatcaaaa aaaattaatt tttttaaata aagatgaaag
    12961 ttattgaaat atagagtatg ataaataaga atttttaata aggtaggttg gctcatgaag
    13021 cagtatcgac agttaaaagg atatttagaa gcaaaaggat attgggagat actagtctat
    13081 ctcttttttg gtggtttggc tacgattgta aattttgtta gttttgctct cgcacgccaa
    13141 tattttgatt taagtatggc cttatccaat tctatttcgt ggttttgttc tgtattgttt
    13201 gcttttgtaa cgaacaaact ttgggtattc cattcaaaat caccgaattt tacacatgca
    13261 cttattgaat gcggtaagtt cttcttctac cgtatccttt cttacggatt ggacatgggg
    13321 gcaatggtat tgttgatcaa tgttatgaac agcaatgagt atgtggcgaa aatcatcact
    13381 caaattattg ttatccttgc gaactatatt ttcagcaagt tgtttatttt taaagaaact
    13441 gaggtttttg aagaagaagt aggtcaaagt gaaaaagacg actaggcata acaattgaag
    13501 aatttccttc tctttgttac tctttgtaat gtagacatta ttaagaaaac attgaggagg
    13561 aattttttta tgacttacac attaccagat ctaccttatg catatgacgc attggaacct
    13621 tatattgacg aagaaacgat gcatctgcat catgataagc atcacaatac ttatgtgacg
    13681 aatttaaatt cagcaattga gaaataccca gaattaggcg aaaaaacaat agaagaatta
    13741 ttatctgata tggacgctat tccaacagat atcaagacag ctgtacgtaa caatggtggc
    13801 ggacatgcta accattcatt tttctgggaa atcatggcac caaatgctgg tggcgaacct
    13861 acaggagaaa taaaagaagc gattaatgaa gcttttggtg atttttcttc ttttaaagaa
    13921 gaattcaaaa aagcagccgc tggacgattt ggttctggat gggcttggct tgtaatggaa
    13981 aatggaaaat tagctattac ctctactgca aatcaagatt ctccattgat ggaaggaaag
    14041 acaccaattc taggtttgga cgtttgggaa catgcttatt atttgaaata taaaaatgtc
    14101 cgaccagatt atattgcagc attctggaat gttattaatt gggatgaagt aaacaaacgt
    14161 ttcgaagcag caaaataacc atttcctccg tcatatcagt cttaaaaata atttggaaaa
    14221 taaaagagcg aaagcttgct agcgttttct aaattgggta taatgttctt gcaaggttac
    14281 ttgcaagaac ttttttggag gatatacatt tatggaacac ggaacggtta aatggttcaa
    14341 caatgaaaaa ggatttgggt ttattacagt agacggcgga gatgacgtat ttgttcattt
    14401 ctcagcaatt caaggagacg gcttcaagag cctagaagaa ggtcaagaag tagaattcac
    14461 gattgttgaa ggtgcacgcg gacctcaagc tgctgaagtg actaaattat aataatttgt
    14521 tgaataaaga cacgacttct tagagagtcg tgtctttttg tctatttatg aagtttttag
    14581 attttttggc taagtaaatg catttgttca cttgacatgt cctaactaat tatcaagcaa
    14641 ggaacatttt cttcgttagt aatggatttt aaagaaattt cagtttttgg atgttgaata
    14701 aaagaccatt tgtgaaacca ctagtataca tactcattac catgtcaatt ctattacttt
    14761 aaaagaaaat cgttttactt tgaagaggag catatctatc cattaaaaga tgatggtaat
    14821 gcaatcacta gttcgagtca ttttaagagc catgcagtga gctggaaata aaacgaatag
    14881 aagatgggtt taacaaaagg accagtcata ggaagaaatt gtcgtgaaat cgaatgataa
    14941 tctgaacggt aggcaaagac tgttaaaaag atgggaaagg tatgtgcgat cattttactg
    15001 cacgattatt tggaaaatac cttgttgttc gctttttctg gaaaaaatac ataaaaaaag
    15061 ataaaacgat tgcgaactgc gtgtattcgg tctttcgatc tttttcacgg tatggtatga
    15121 tagttaagaa ttttgagaaa ttttaaaaaa taataatcgt tacacaaaaa tgttttttaa
    15181 agttttctta tgattaaaaa gtgaatttgc tgattgaaca gttatatgag acaatatttt
    15241 agaaaatgtg aaacagaggt gtattatgaa gcgatggcaa aaagttctat taaccttgtt
    15301 aggggtattc gttttgctga ttgccggatt atcagcatac ggaatcagat ttatgggaga
    15361 agcgagccaa acagtaaaca aaatttctaa aaactctaat cgaatatcct ccaaacgttc
    15421 tgagagagta agcattgatg acaaagaacc tttttcggtt ttgctattag gtttagatac
    15481 cggtggatta ggaagaacgg aacaaggacg ttctgatacg atgatggtag taacagttaa
    15541 tccgaaacaa aaaaaatcaa cgatcatcag tttggaccga gatatttata cgaatatcgt
    15601 aggttatgga acggtagata aattaaatca tgcctacgca tttggtggtg tcgaaatggc
    15661 catggattca attgagcaac tgctcgatat tcctattgat cattatgtaa cgatcaattt
    15721 ggatggcatg gaagatttga tcaatgcggt cggtggcatc aaagtgaata ataaaattga
    15781 ctttactttg gatggtgtcc atgtaccagt aggtgaacaa acattgaatg gagaaaaagg
    15841 tttagcctat tctcgaatgc gacacgaaga tccagaaggc gatatcggtc gacaacgtcg
    15901 tcagcgggaa gtagtgacga aaatcgtcaa taaagtttta agtttagatg gcgtaagcaa
    15961 ttatagaaaa attttaaaag cagttgagaa caatgtgacg acggacttag attgggatga
    16021 catgcttgat gtagcgacga actacacccc ggcgtttgaa acaatcaaac aagaccagtt
    16081 gcaaggcgaa ggacaaatga tcaatgatat ctactatcaa attttaggaa aaaatgatct
    16141 tctttcgatc caaaatgaac tgaagaaaca attaaatata aaaacaagcg atacattacc
    16201 taatttgaaa aatgagaatg cttcaatgat gttctttgat gattcggaag atggcgattc
    16261 gacaaacggt gatggatcga atagtgaagg gtacaccgat tattcttcct attcagaaaa
    16321 cacatatgat cagtaccaag atccgaatac ctattatcag caaacagaag atacaagtca
    16381 gtactataca ccaagtcaag atactcaaag ttatgatcct tacgctggac aggaacaaaa
    16441 tggttatggt tatgaccaga ataatgagta tggaacaaca agtgggtact agaaaaaaat
    16501 agtttggtta ataaagagtg ccacctaaat tagacaaaaa atctaactta ggtggcactt
    16561 tttctatgtg ttaaattaca ggacgtctct ttttagcgct tagtttaaaa aataaattga
    16621 ataattcaat agagttgcga atagtaccca taaataataa ggaatcatga gccacattgt
    16681 caggcgatga tgcagccaca actgatacat aagcactgac aagaaaataa ctaaaagtgt
    16741 aatatcgatt gctgcaatga aaatatttct taaattaaaa aatagagcag accatataaa
    16801 attaaaaatg aactgaatca caaaaagacc gacaagattg tttcgctgtt ttttgttgtt
    16861 gtgattaagt aacaaataaa gagaaatacc catcaatata tacagtaagg tccacatcgg
    16921 cccaaatatc cagctaggtg gtgcaaatgg tggaagttgc aaagaatagt aatattttcc
    16981 gggatttccg ctaagcaacc ctgataaaaa gcctaatatg acaattccta taacacaaac
    17041 ccaaaaacga atatctttta tcatcttcat tttactaact cctttctata atattagttt
    17101 accatgaagg ctcacagcaa gacctgtatt tagtttgtga agtaagaaaa aaattgataa
    17161 ttgttgttat taaaaagttg aacatttgtt tatatgaatg atgtttttga aaaagatcat
    17221 atgatttgat tctcatgttt tctatttgta cttttctcgt ctacttctgt tataatctgc
    17281 atcttacttt tttcaccttt tttacttcaa cataaaatta taaaagcata atgtttgtgt
    17341 taatacaata aagcgtttac aatgtggtta tcaaatgaag gaggtttcta catgaaaaag
    17401 attagtacgt tattgtttat tgggttagtt agtttagggt tgttcggttt tagcattcca
    17461 ggttttgcgc atggttatat tacttctcca ggaagccgtg catacctagg aacatcagca
    17521 gcaggaaact taaatcaaaa tgtcggaaga gcacaatggg aaccgcaaag tatcgaagct
    17581 accaaaaata cattcattga tggaaaaatt gctagtgcag gagtcagcgg ctttgagcct
    17641 ttagatgaac agacatctaa cagatggcat aaaaacatgg ttaatccagg aacattaaat
    17701 attacttgga atttaacagc acaacatcgg acaagtactt gggattacta cattaccaaa
    17761 ccaacttgga atcctaatca acctttgaaa tttagtgatt ttgaattgat cacaaaaata
    17821 gacgacaaag caactgttcc accaaaaaca gtcaaccaaa cgatcacact tccgcaggat
    17881 cgaaaaggat ataatgtgat tttagcagta tggaatatca gcgatacgac aaatgcattt
    17941 tatcaagtga tagacgtgaa tatccaataa taatcaaaaa gggctgtgac aaaaactatg
    18001 tcacaaccct ttttttaaat aaatagtgtc ctcctgttaa ctcttcggtc acaagtacag
    18061 tattcataaa acatgaagag ctatttttgt ttggctgttc ttattacttg atacagggca
    18121 gactttttca caacctcttt catttagtca gcgagtctcg tcatcttcac gacaacttca
    18181 caacgtgcag tctgaggaaa catgtcgacg gactgtaaat attcaatctt gaacttttta
    18241 cttaaat
//
LOCUS       DACEEZ010000054        17944 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20313_2017
            SAMN12639966-rid9879223.denovo.054, whole genome shotgun sequence.
ACCESSION   DACEEZ010000054 DACEEZ010000000
VERSION     DACEEZ010000054.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639966
            Sequence Read Archive: SRR10023158
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 17944)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17944)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741683.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741683.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:44:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,016
            CDSs (total)                      :: 2,962
            Genes (coding)                    :: 2,889
            CDSs (with protein)               :: 2,889
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 36 of 73
            Pseudo Genes (incomplete)         :: 29 of 73
            Pseudo Genes (internal stop)      :: 18 of 73
            Pseudo Genes (multiple problems)  :: 8 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17944
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639966-rid9879223.denovo.054"
                     /isolate="E20313_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(26..1522)
                     /gene="lysS"
                     /locus_tag="IWO89_001867"
     CDS             complement(26..1522)
                     /gene="lysS"
                     /locus_tag="IWO89_001867"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007208013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HAQ0456598.1"
                     /translation="MTQEQQHQVEDLNDQMLVRRQKMEQLREDGIDPFGKRFERTHNS
                     EELHELFDPRTKEELAEMGLTASVAGRMMTRRGKGKAGFAHLQDREGQIQIYVRKDQV
                     GDEAYEVFKHADLGDFFGVTGQIMKTDTGEVSIKASEITILSKALRPLPDKYHGLTNI
                     EQRYRQRYLDLISNRESFDRFMKRSQIISEIRRYLDGNGYIEVETPVLHNEAGGAAAR
                     PFITHHNALDIDLYLRIALELHLKRLIVGGMEKVYEIGRVFRNEGIDTTHNPEFTMLE
                     AYTAYTDFNDVMNLTEGIIRNAAEKVLGTAKITYDGQAVDLESDFKRIHMVDAIKEQT
                     GVDFWQEMTLEEALALAEKHNVEVTEAMGVGHVINEFFETFVEETLTQPTFVYGHPVE
                     VSPLAKKNPEDPRFTDRFELFIVGREFANAFTELNDPIDQRERFEAQEKERELGNDEA
                     HGVDEDFLEALEYGMPPTGGLGIGIDRLVMLLTDAQSIRDVLLFPTMR"
     gene            complement(1633..2637)
                     /gene="dusB"
                     /locus_tag="IWO89_001868"
     CDS             complement(1633..2637)
                     /gene="dusB"
                     /locus_tag="IWO89_001868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine synthase DusB"
                     /protein_id="HAQ0456599.1"
                     /translation="MEHTWKIGNVEIPNRVVVAPMAGITNSAFRVTVKEFGAGLVVCE
                     MISDQGIHFRNKKTLEMLHIEETEHPLSLQIFGGNKDSLVEAAQFVEENTAADIIDIN
                     MGCPVNKVIKAEAGAKWLLDPEKVYEMVQAVSSAVNIPVTIKMRIGWDEEHVFAVENA
                     LAAQSAGASAIAMHGRTRVQMYEGKADWEVLKEVKKHLTIPFMGNGDVKTPEDAKWML
                     DYVGADGVMIGRAALGNPWMIHRTQHYLETGELIPEPTPAEKIATAKLHLQRLADLKG
                     EKVACREFRTHAAYYLKGIPRAAKVKVAINQTEKQQEINDLLDQFVEKAEKVLLKQTL
                     "
     gene            complement(2638..3525)
                     /gene="hslO"
                     /locus_tag="IWO89_001869"
     CDS             complement(2638..3525)
                     /gene="hslO"
                     /locus_tag="IWO89_001869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp33 family molecular chaperone HslO"
                     /protein_id="HAQ0456600.1"
                     /translation="MKDYLVKALAHDGFVRAYAVQATNTVAEAQRRHDTWHTSSAALG
                     RTLVGSLLLGATLKGEDKLTVRIQGDGPATGIVVDANGKGDVKGYIKNPHLSLPLNSE
                     GKIDVRGAVGTQGMFTVTKDLGLKEPFSGQTPIVSGEIAEDFTYYLAISEQIPSAVGL
                     SVLVNTDDSIKTAGGFMIQIMPGADEETISKIEEQLKTTPRISTLLDEGQTPEEILKG
                     LLGTEELNILEKMPVQFKCDCSKEKFAEAIIALGAEEIQAMIDEDHGAEAVCSFCNNK
                     YKYSEEELDGLKKEALGGI"
     gene            complement(3742..5853)
                     /gene="hflB"
                     /locus_tag="IWO89_001870"
     CDS             complement(3742..5853)
                     /gene="hflB"
                     /locus_tag="IWO89_001870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HAQ0456601.1"
                     /translation="MNKKNNGMMKNALYYVLVILAMVMVVYFIFGNNGQRSSDIEYSK
                     FTEQLQAGKIKEFEVQPANGVYKITGEYKEQQTVANSGGLAILGSTDTTTTHFTTIIL
                     PNDTTLSQVTELAKENKVATTVKEESSSGIWVSLLLSFLPIVIIIFFFYMMMGQQGGG
                     GGGGGRVMNFGKSKAKEADKKANRVRFSDVAGAEEEKQELVEVVEFLKDPRRFVELGA
                     RIPAGVLLEGPPGTGKTLLAKAVAGEAGVPFYSISGSDFVEMFVGVGASRVRDLFETA
                     KKNAPAIIFIDEIDAVGRQRGAGMGGGHDEREQTLNQLLVEMDGFDGNEGVIVIAATN
                     RSDVLDPALLRPGRFDRQILVGRPDVKGREAILRVHARNKPMADDVDLKVVAQQTPGF
                     AGADLENVLNEAALVAARRNKKKIDASDIDEAEDRVIAGPAKKDRVISKKEREMVAYH
                     EAGHTIVGLVLSRARVVHKVTIIPRGRAGGYMIALPKEDQFLMTKEDMFEQIVGLLGG
                     RTAEEIIFNVQSTGASNDFEQATALARSMVTEYGMSDRLGPVQYEGNHQVFVGRDYGQ
                     TKAYSEQVAFEIDQEVRKILMEAHDKAREIIEAHRAQHKLIAEKLLEFETLDAKAIKS
                     LFEHGVMPEGTESNDYPSEKEAKTFEEAKRALEEKDAEKQAEEKRDFEEAKHELNEEN
                     KAEHDSDSQQSEDNHSDDQYK"
     gene            complement(5943..6488)
                     /gene="hpt"
                     /locus_tag="IWO89_001871"
     CDS             complement(5943..6488)
                     /gene="hpt"
                     /locus_tag="IWO89_001871"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine phosphoribosyltransferase"
                     /protein_id="HAQ0456602.1"
                     /translation="MLEKDIERILISQEEIQVRCKELGKELTEIYQNTNPLVVGVLKG
                     AVPFMADIVRSIDTYLELDFMDVSSYGNATVSSGEVKIVKDLDTNVEGRDLLIVEDII
                     DSGRTLAYLVDLFKYRKAKSVKIVTLLDKPEGRVVNIEADYVGFNVPNEFVVGYGLDY
                     AEAYRNLPYIGVLKPSVYQSN"
     gene            complement(6488..7933)
                     /gene="tilS"
                     /locus_tag="IWO89_001872"
     CDS             complement(6488..7933)
                     /gene="tilS"
                     /locus_tag="IWO89_001872"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HAQ0456603.1"
                     /translation="MNVEKGGKKAPLLQQSFIRHGNEQGFWQQGSRILAAVSGGVDSM
                     VLLHLLLQVQRQIGFSLGIVHINHQLREESAEEAAYLRSYCEEKNLPLYVSIWEDPAK
                     SAIETEARNFRYQEFTRVMEEGQYDTLMTAHHGDDQIETVLMKIVRGGQLNTFSGIKE
                     VQPFATGRLIRPLLSFSKEDLYTYAAESQLVYFEDQTNQLLDIQRNRLRHLVVPQLKQ
                     ENTQALRHFQQFSQQIQWADQVIQKYMGQLIEKEVEQWEEQFRFSAEMIEEMTDAERY
                     YFFYSFFDKAFSKTGIILKEQQMESILDQWQQKKSQWQLSIGNNWILKREYHFVVLSK
                     KEKQATSQTEDQRLLLIPEQGIYLNETEWIGLVKPENAGDIKATDSWSLFSHDIWIPA
                     DVQLYVEKRKAGDRIKLTENLTKKVSRYFIDQKVPNSQRQHAWIITDAKRNIYSLIPF
                     AFSYLSIREETDKIHYILLYKYQKEAIGRRT"
     gene            complement(8053..8523)
                     /locus_tag="IWO89_001873"
     CDS             complement(8053..8523)
                     /locus_tag="IWO89_001873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein S1"
                     /protein_id="HAQ0456604.1"
                     /translation="MSIEVGAKLQGKVSGITNFGAFIDLGAGKTGLVHISEVSNGYVK
                     DIHDVLSVGDEVTVKVISVNDDGKIGLSIRKAQEQPAESGEESRPKKYQQKDPRDNRN
                     RPAQKKSFSRSQPSAQKEDFDSLMSSFLKDSDDRLSSLKRNTEGKRGGRGGRRS"
     gene            complement(8569..8994)
                     /locus_tag="IWO89_001874"
     CDS             complement(8569..8994)
                     /locus_tag="IWO89_001874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum formation initiator family protein"
                     /protein_id="HAQ0456605.1"
                     /translation="MTKKSNKIAALDTEYAKKKYAEFQQQQRQLIFRRRRLAAIFVGA
                     LVIFAIIGVQIFHDMQRMHQLNELRVEANTEMKEVNADVDQLHQDVSLLKDDEYVAKL
                     ARSKFFYSKDGEKVYPILESNTDSSTNETDQSTSSTTAK"
     gene            complement(9061..9330)
                     /locus_tag="IWO89_001875"
     CDS             complement(9061..9330)
                     /locus_tag="IWO89_001875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding S4 domain-containing protein"
                     /protein_id="HAQ0456606.1"
                     /translation="MRLDKFLKISRIIKRRSVAKEVADKGRIQINGKLAKSSSDVKVG
                     DILKVQFGNKTLEVKITELHESTKKEDAQKMYEIISENRTETLEK"
     gene            complement(9341..10936)
                     /locus_tag="IWO89_001876"
     CDS             complement(9341..10936)
                     /locus_tag="IWO89_001876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HAQ0456607.1"
                     /translation="MDHKEMRRVMQGAFVLTIASFIAKVLSALYRIPLQNLVGDEGFY
                     VYQQVYPIYGIAMTLALSGLPQFISKYVAERKNPFERKQALQNLYPLVFWTGVFLWLF
                     VFAFSSWIAWMMGDIQLKPLIQVVSFTFLLMPGLSFYRGNFQGRFLMEPTAVSQVVEQ
                     FVRVFVIVCSAFAFRKLGWTVYQTGTAAMSGAVFGGLCAYGILHYYEQKIHGGALSFR
                     RFPLFKQPSKPLIRRFLVEGGLVSIYSGLLIFFQLIDSFFVKNALEVYGLSEYGAKIA
                     KGVYDRGQPLVQLGLVIATALSATFLPALTRHLTNRIYRQFLQTAKIYLRLTTALALA
                     ASLGLALLLPYINYALFKDYAGNAALVLFVFSIAFTAVIQAYQSIAQSKNSFRPSLKG
                     AGWGLLVKGLTTSFLTGLLGTAGASLSTLLGLGVTLWYFVRIETKELNAFWKERHFGR
                     KLMFSLLCMTVVLLLYYGVLNIVFGPVTHRSTAFFVSVIGVGIGASVFIYAVIRLRLF
                     TVREWLMLPFGKKILRLNKKIKK"
     gene            complement(10951..14472)
                     /gene="mfd"
                     /locus_tag="IWO89_001877"
     CDS             complement(10951..14472)
                     /gene="mfd"
                     /locus_tag="IWO89_001877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HAQ0456608.1"
                     /translation="MDIIQLVGKDPQVKEWVSNLEEKMPRQLITGLAGSAKTLLFARA
                     FKKTNKNMIILMPNLYYANQLAEDLQHVIPQDQIHLFPVDEVLSAEMAFSSPEARGER
                     VATLNALQEKKAGIYLLPVASLHKRLPNKKTWLQAQLNWHIGDEIDVENLPRQLTLMG
                     YVREEMVAKPGEFSIRGSIIDIYPLTTTYPVRVELFDVEIDSMRYFEAETQRSIEKTE
                     EIWVSPTSEQVYGPEDLEHGIEQMQKMLEKRLAATTEEADREFLQDYFGQLTSSWQAG
                     VPTEEAKFYSDLLYQESVTILDYFPSNSLLVVDDYQRIMETNREIEREAAEWQTQKIS
                     ELRVFSEQTFSADIQKIVQKEKFTTTFFSLFQKGMGNLRFQAIHNFQYRTMQQFFGQM
                     PLLKTEMDRWRKQKQTVVIFIPTKDRIRKAEQMLRDEDILVVETEKNALIRGQVQLVE
                     GALQAGFELPQEKIVAITEKEIFQKTTKKQTRRQTVTNAERLKSYNELKAGDYVVHAN
                     HGIGKYIGMETLEVDGVHQDYMTILYQNDDKLFIPVTQLNLIQKYVASEAKAPRINKL
                     GGSEWTKTKRKVSSKIEDIADDLIKLYAARESEKGYAFGPDDAYQKEFENAFPYSETD
                     DQLRSAAEIKRDMEKEKPMDRLLVGDVGYGKTEVALRAAFKAIKESKQVAFLVPTTIL
                     AQQHYETMLERFEGFPVNIGLLSRFRTRKQQKETIEQLRTGQVDIVVGTHRILSKDIE
                     FSDLGLLIIDEEQRFGVKHKERLKQLRAQVDVLTLTATPIPRTLHMSMLGVRDLSVIE
                     TPPANRYPIQTYVMEKNPGAIREAIHREMGRGGQVFYLYNRVETIEQKVEEIQELVPE
                     ARIGYAHGQMTEAQLENTLFEFIEGQYDVLVTTTIIETGVDIPNANTLFVENADYMGL
                     STLYQLRGRVGRSSRVAYAYFMYEQQKILNEVSEKRLQAIKDFTELGSGFKIAMRDLS
                     IRGAGNLLGAQQHGFIDAVGFDMYSQMLSEAVSRKQGKNSQVEKTTVEIDLGVDAYLP
                     ETYVADQRQKIEIYKRIRELDSQEMLDELEDDLLDRFGEYPEEVAHLLTIGQIKMDGD
                     RALLETIRKQEAHIVFTLSKVGTKRYSVEQLFEALTATKLKASLGVEKEQMVIRLTIP
                     NQMEEAVWLQEIQKFVKALREQKYLSA"
     gene            complement(14489..15049)
                     /locus_tag="IWO89_001878"
     CDS             complement(14489..15049)
                     /locus_tag="IWO89_001878"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HAQ0456609.1"
                     /translation="MKMIVGLGNPGTKYQYTKHNIGFMVVDKIAREHQATFKKNPFEA
                     EVAEFFHNGEKILLVKPQTFMNESGRAVGPLMTYFGIYPEELVVIYDDLDLAVGKIRL
                     RQKGSAGGHNGIKSIISHLNTNVFDRIKVGIGRPEGKKTVVQHVLSPFSKENQPLIEE
                     SMCQSVKAVEYLIEGHSFVDAMNRFN"
     gene            15402..16376
                     /locus_tag="IWO89_001879"
     CDS             15402..16376
                     /locus_tag="IWO89_001879"
                     /EC_number="1.1.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate dehydrogenase"
                     /protein_id="HAQ0456610.1"
                     /translation="MTAAAEKKDHQKVILVGDGAVGSSYAFALVTQNIAQEVGIVDIN
                     TAKTEGDAIDLSHALAFTSPKKIYSATYADAHDADLVVITAGAPQKPGETRLDLVNKN
                     LKINRDVVTQIVESGFNGIFLVAANPVDILTYSTWKFSGFPKERVIGSGTSLDSARFR
                     QALAELVDVDARNVHAYILGEHGDSEFPVWSHANVAGLQIYEWVKNNPEIDEEAMVNL
                     FFGVRDAAYTIIEKKGATFYGIAVALARITRAILDDENAVLPLSVFMNGEYGLNDIYI
                     GAPAVINRQGIQKVIEIPLSDSEQDRMAASAKQLKEILDEAFAKLDAE"
     gene            16546..>17944
                     /locus_tag="IWO89_001880"
     CDS             16546..>17944
                     /locus_tag="IWO89_001880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase family protein"
                     /protein_id="HAQ0456611.1"
                     /translation="MTRSSHRKKSKHVALLSVLGVLLVLISFYTYRSTYYTKHFLPNT
                     EINGINVSNLTVEKANEKLKEAYSDKKLAIKEDGKVWQEVPKSELGYKDDFTSELSPI
                     LNEQNGWTWGMTYVSAAEKQEIDPVSQNHQKLDTTVQTLTTKLTDLNKDRTPTTDATI
                     EKAGDSFVIKPEVNGNTIDVDAAVKQLKSAVNSGKDTIELTEFKEKPKVTSEDSSLKE
                     QLASMNAIANVKATYSINGETFQIPSSDIMSWLTYNDGKVDLDTEQVRQYVTDLGTKY
                     NTSTNDTKFKSTKRGEVTVPVGTYSWTIQTDSETEALKKAILAGQDFTRSPIVQGGTT
                     ADHPLIEDTYIEVDLENQHMWYYKDGKVALETDIVSGKPTTPTPAGVFYVWNKEEDAT
                     LKGTNDDGTPYESPVNYWMPIDWTGVGIHDSDWQPEYGGDLWKTRGSHGCINTPPSVM
                     KELFGMVEKGTPVLVF"
BASE COUNT         4869 a         3973 c         3238 g         5864 t
ORIGIN      
        1 aattccttag acagctttta ttcgattatc tcattgttgg gaatagcaaa acatcccgaa
       61 ttgattgtgc atcagttagc aacattacta atctgtcgat cccgatgcct agaccaccag
      121 ttggcggcat accgtattct aaggcttcca agaagtcttc gtctacaccg tgagcttcgt
      181 catttcctag ttcacgttct ttttcttgcg cttcgaatct ttctctttga tcaattggat
      241 cgttcagttc tgtaaaggca ttagcaaatt cgcgccccac tatgaatagt tcgaatcgat
      301 ctgtgaatcg aggatcttct ggatttttct tcgctaaagg cgaaacttcg actggatgtc
      361 cgtatacaaa cgtcggttgt gtcaatgttt cttcaacaaa tgtttcaaag aattcattga
      421 taacatgtcc gacacccata gcttcggtta cttctacatt gtgtttttcg gctaaagcta
      481 atgcttcttc caatgtcatt tcttgccaga agtctacacc agtttgttct ttgattgcat
      541 ctaccatatg gatgcgttta aagtcacttt ctaagtcgac agcttgtcca tcatatgtga
      601 tttttgctgt tcctagtact ttttcagctg cgttacggat aataccttct gtcagattca
      661 ttacatcatt gaaatcagta tatgctgtgt atgcttctaa catcgtaaat tctggattat
      721 gtgtagtatc aattccttca ttacggaaaa cgcggccgat ttcataaact ttttccattc
      781 cgccaacaat tagtcgtttt aaatgaagtt ctagcgcaat acgcaaatat aaatcaatgt
      841 ccagtgcatt atgatgcgtg ataaatgggc gtgcggcagc tccacctgcc tcattgtgca
      901 atactggtgt ttctacttca atatagccat tgccatctaa atagcgacgg atttcgctga
      961 taatttggct tcgtttcata aatcggtcaa agctttcgcg attactgatt aaatccaagt
     1021 agcgttgtct gtagcgttgt tcaatattcg ttaagccatg gtatttatct ggcagtggac
     1081 gaagtgcttt tgacaaaatc gtgatttctg aagctttgat agaaacttcg cctgtgtctg
     1141 ttttcatgat ttgtccagtt acaccaaaga aatctccaag gtctgcatgt ttgaatactt
     1201 cataggcctc atcacctact tggtcttttc ttacataaat ttgaatttgg ccttcacgat
     1261 cttgtagatg cgcaaatcca gctttccctt ttcctcggcg agtcatcata cgaccagcta
     1321 cactagcggt caatcccatt tctgctagtt cttcttttgt tcttggatca aatagttcat
     1381 gcaattcttc tgagttgtgc gtgcgttcaa atcgttttcc aaatggatcg attccatctt
     1441 cacgcaattg ttccattttt tgacgacgta caagcatttg atcatttaaa tcttctactt
     1501 gatgttgttg ttcctgtgtc acaaaagttc ctccattctc atgttaatct ctttcctata
     1561 atgccaatga aacacaaaaa aagcaagccg aacttggaat ttcggcttga aaattcacga
     1621 aaaacttaga aatcacaacg tttgtttcaa tagtactttt tccgcttttt ctacaaattg
     1681 atccagcagg tcattgattt cttgctgttt ttctgtctga ttgattgcta ctttcacttt
     1741 cgctgctcga ggaatccctt ttaaataata agccgcatgt gttctgaatt ctctacaagc
     1801 tactttttct cctttgagat ctgctaagcg ttgtaaatga agtttagctg tagcaatttt
     1861 ttcggctggt gttggttcag gaatcagttc ccctgtttcg agataatgct gtgtgcggtg
     1921 aatcatccat ggattgccca gcgctgcacg accaatcatc acaccgtctg caccgacata
     1981 gtcaagcatc cattttgcat cttctggcgt tttgacatcc ccgttcccca taaatggaat
     2041 cgtcaaatgt tttttcactt ctttcaatac ttcccagtcc gcttttcctt catacatctg
     2101 aacccgtgta cgtccatgca tagcgatggc acttgctcca gctgattgtg ctgctaatgc
     2161 attttctaca gcaaagacgt gctcttcatc ccaaccaata cgcatcttga ttgttactgg
     2221 gatatttact gctgaagaaa cagcttggac catttcgtat actttctcag gatctaaaag
     2281 ccattttgct cctgcttctg ctttgattac tttatttact ggacagccca tattgatatc
     2341 tattatatcg gcagctgtgt tttcttcgac aaattgagca gcttcaacta gactatcttt
     2401 atttccgcca aatatctgta aacttaatgg atgttctgtt tcttcaatgt ggagcatctc
     2461 taaagttttt ttgtttctga aatgaatacc ttggtcactg atcatttcac atacgactaa
     2521 gcccgcacca aattctttga cagtgactcg aaatgctgaa tttgttattc cagccatcgg
     2581 tgcaacaacg acacgattag ggatttccac attgccaatt ttccatgtgt gttccactta
     2641 aatccctcct aaggcttctt tcttcaatcc atctaattct tcttcgctat acttgtattt
     2701 gttattacag aaagaacaaa ccgcttctgc accgtgatct tcgtctatca ttgcttggat
     2761 ttcttctgca cctaacgcga taattgcttc tgcaaatttt tcttttgaac agtcacattt
     2821 gaattgaaca ggcatttttt ccaaaatgtt caattcttct gtaccaagca atcctttcaa
     2881 tatttcttct ggtgtctgtc cttcatctaa gagggtagag attcttggtg tcgtttttaa
     2941 ttgttcttcg atcttgctga ttgtttcttc atcggcacct ggcatgattt ggatcatgaa
     3001 tcctcccgcc gtcttgatgc tgtcgtcagt attgaccaat acactcagtc caacagctga
     3061 agggatttgt tctgagatag caagatagta agtaaaatct tcagcgattt ctccagaaac
     3121 aatcggcgtt tgcccagaaa aaggttcttt taatcctaag tcttttgtga cagtaaacat
     3181 tccttgtgtt ccaactgctc cccgcacatc gattttacct tcactattta gaggaagact
     3241 gagatgcgga tttttgatgt aacctttcac atctcccttt ccatttgcat ctactacaat
     3301 tcctgttgct ggaccatcgc cttggattcg aacagttagt ttgtcttctc ctttgagcgt
     3361 cgcaccaagt agcagactac cgaccaatgt ccgtcccaaa gcagctgagg aggtgtgcca
     3421 agtatcatga cgtctttgtg cctccgctac ggtatttgtt gcttgaacgg cgtatgctcg
     3481 gacgaatccg tcgtgagcaa gtgccttaac caaataatct ttcatttgtt atgtactcct
     3541 ttttccgttt ttaaactcat agtgaatcat actagcaact actgcttttt tcaactttgg
     3601 aatgcttctt ttaaaaataa ttcagaatca tctagaaaac aggacatcac cctatttaca
     3661 acttaaataa agaaatctcc cgaaaaccgg tttttgttcc ggttttcggg agtcatatca
     3721 gatcaattga cctatatttt tttatttgta ttgatcgtct gaatgattat cttctgattg
     3781 ttgagaatct gaatcatgtt ctgctttatt ttcctcattc agttcatgtt ttgcttcttc
     3841 aaaatcacgt ttttcttccg cttgtttttc agcgtctttt tcttccaaag cacgttttgc
     3901 ttcttcaaaa gttttcgctt ctttttcgct tggataatca tttgactcag taccttcagg
     3961 catcacaccg tgttcaaaca atgacttgat tgctttggca tccaatgttt cgaattccaa
     4021 aagtttttca gcaatcagtt tatgctgtgc acgatgagct tcgatgattt ctcgagcttt
     4081 atcatgagct tccatcaaga tttttcgtac ttcttgatca atctcgaaag cgacttgttc
     4141 cgagtaagct tttgtttgac catagtcgcg gccaacaaac acttgatggt ttccttcata
     4201 ttgaacagga cctaaacgat cactcattcc atattctgtt accatgcttc gtgctagagc
     4261 tgtcgcttgc tcaaagtcat tggaagcacc agttgattgg acattgaaga tgatttcttc
     4321 agctgtacgt ccaccaagta agcctacgat ttgttcaaac atatcttctt tcgtcatcaa
     4381 gaactgatct tcttttggta aagcgatcat atatccgccg gcgcgtccgc gagggatgat
     4441 cgttactttg tggacgaccc gagcgcgact caatactaat ccgacaatcg tatgaccagc
     4501 ttcgtgataa gcaaccattt cgcgttcttt tttgctgatt acgcgatctt ttttagctgg
     4561 acctgcaatc acgcgatctt ccgcttcatc gatatcagaa gcatcgattt tctttttatt
     4621 tcttctagca gctactaacg ctgcttcatt caagacattt tctaaatctg ctccggcaaa
     4681 acctggtgtc tgttgagcca caactttcaa gtcaacgtca tctgccatcg gtttattccg
     4741 tgcatggact cgcaagattg cttcacgacc ttttacatct ggacgtccaa ctaaaatctg
     4801 gcggtcaaaa cggcctggac gtagtaacgc tgggtctagt acatcagaac ggttggttgc
     4861 agcaatgacg attactcctt catttccgtc gaagccatcc atttctacaa gcaattggtt
     4921 caatgtttgt tcgcgttcat catgtccgcc gcccataccg gcaccacgtt gacgacctac
     4981 cgcatcaatt tcatcaatga aaatgatagc tggtgcgttt ttcttcgctg tttcaaacaa
     5041 atcacgaaca cgacttgctc caacaccgac aaacatttct acgaaatcag aacccgagat
     5101 cgagtaaaac ggtactccag cttctccggc aacagctttt gctaataagg ttttacctgt
     5161 tcctggaggt ccttctaaaa gtacacctgc cgggatacgg gcacctaatt caacaaatcg
     5221 gcgaggatct ttcagaaatt caacaacttc aactagttct tgtttttctt cttccgcacc
     5281 cgctacatca gaaaaacgta cgcgattagc ttttttgtct gcttctttgg cttttgattt
     5341 accaaagttc atgacacggc cgccaccgcc accgccgccg ccttgttgtc ccatcatcat
     5401 gtaaaagaaa aagatgatga ttacgattgg caagaagcta agcaataggg aaacccatat
     5461 accgctgctt gattcttctt ttactgttgt agcaactttg ttttcttttg ccagttctgt
     5521 tacttggctc aatgttgtat catttggcaa aatgattgtt gtgaaatggg tagtagtcgt
     5581 atctgttgaa cctaagatag ctaacccacc gctgttagcg acggtctgct gttctttgta
     5641 ttcaccagtg atcttatata caccatttgc aggctgcact tcaaattctt tgatcttgcc
     5701 tgcttgtagt tgttctgtga attttgagta ctcgatatct gacgatcgct ggccgttatt
     5761 accaaaaatg aaataaacga ccattaccat cgctaagatt acgaggacat aatacaaggc
     5821 atttttcatc atgccgttgt tctttttgtt catagttgcc ctccctaagc gtcaatttaa
     5881 tttaacagtt ttttatcaat cctgaccatt ctattgtacc ataatttaat tgagatacag
     5941 cattaatttg attgataaac actaggtttc aatacaccga tgtacggaag gttacgatat
     6001 gcttctgcat aatccaagcc ataaccgacc acaaattcat ttggtacgtt gaagccaaca
     6061 taatctgctt cgatatttac tactcttcct tcaggtttgt caagtagtgt aacaattttg
     6121 actgattttg ctttgcggta tttgaaaaga tccactaaat aagcaagtgt tcttccgctg
     6181 tcaatgatat cttcaacgat caacagatcg cgaccttcta cattcgtatc taagtctttg
     6241 acgattttta cttcccctga cgagactgtt gcattcccgt agcttgaaac atccatgaaa
     6301 tccaattcca aatacgtatc gatagaacga acgatatctg ccatgaatgg taccgctcct
     6361 tttaagacac ctactactag gggattggtg ttttggtaaa tttctgttaa ctctttgcct
     6421 agttctttgc aacgaacttg tatctcttct tgtgagatca aaattctctc aatatccttt
     6481 tccaacatca ggttctcctt ccaattgctt ctttttgata tttgtaaaga agtatgtagt
     6541 gtattttatc agtttcttca cgaatactca aatatgaaaa ggcaaaaggt atcaagctat
     6601 atatatttct tttggcatct gtaatgatcc aagcatgctg cctttgtgaa ttaggtactt
     6661 tctgatcgat aaaatatcta gatacttttt ttgtgagatt ctctgttaat ttaatgcggt
     6721 ctcctgcttt tcttttttcg acatacagtt gcacatcagc aggaatccaa atgtcatgag
     6781 aaaatagaga ccatgaatct gttgctttga tgtcccctgc attttcaggt ttgaccagac
     6841 caatccattc tgtttcattc aaatatattc cttgttctgg aatcagcaac aagcgctggt
     6901 cttctgtctg gctagttgct tgtttttctt ttttagagag tacaacaaaa tgatactctc
     6961 ttttcagtat ccaattattg ccaatactga gttgccattg gctttttttc tgttgccact
     7021 ggtctaaaat cgactccatt tgttgttctt tcaaaataat tcctgttttt gagaatgctt
     7081 tgtcgaaaaa ggagtaaaaa aaatagtatc gttcagcatc tgtcatttct tcaatcattt
     7141 ctgcagaaaa tcgaaattgt tcctcccact gttcgacttc cttttcgatc aactgtccca
     7201 tatatttttg aatgacttgg tctgcccatt ggatctgttg cgaaaattgc tggaaatgtc
     7261 tcaacgcctg tgtattttct tgtttcaatt gaggaactac caaatgacga agccgatttc
     7321 tctggatatc taacaattgg tttgtctgat cctcaaagta aacaagctgc gattccgctg
     7381 cataagtata taggtcttct ttgctaaaac tcagcaacgg acgaatcagt ctgcccgtag
     7441 caaatggttg tacttctttg attccagaaa aagtatttaa ctgtccccca cgaacgattt
     7501 tcattaacac cgtctcgatt tgatcatctc cgtgatgagc agtcatcaac gtgtcatatt
     7561 gtccttcttc catcacgcga gtgaattctt gataacgaaa gttacgagct tctgtttcta
     7621 tggcggattt ggctggatct tcccaaatac ttacatataa agggagattc ttctcttcac
     7681 aatatgagcg aagataggca gcttcttctg ctgattcttc tcttaattgg tgattgatgt
     7741 ggacgatacc taaagaaaaa ccgatttggc gttgcacttg taaaagtaaa tggagcaaaa
     7801 ccatcgaatc aacaccacct gataccgctg ctaaaattct cgatccttgt tgccagaatc
     7861 cttgttcatt cccgtgccga ataaatgact gctgtaatag cggtgctttt ttcccgcctt
     7921 tttcaacgtt catcctttcc acaccctttc ttcctaaaaa aaagagagcg ggaaaacgtc
     7981 taaaacgaca attaacctgc cctctgttga tactatgacc caaatagtcg cactatcatt
     8041 atttgcggct ttttagctgc gacgtccgcc acggccaccg cgctttcctt ctgtgtttcg
     8101 cttcaatgaa gataagcggt catcgctatc tttcaaaaat gaactcatca atgaatcgaa
     8161 gtcttctttt tgagctgaag gttgactgcg agaaaaagat tttttctgtg ctggacgatt
     8221 tcggttgtca cggggatctt tttgttgata ttttttagga cggctttctt ctccagattc
     8281 agcaggttgt tcctgcgctt tccggataga tagtccgatt tttccatcat catttactga
     8341 aataacttta actgttactt catctcctac acttaaaaca tcatggatgt cttttacata
     8401 gccatttgac acttcgctaa tatgaactag acccgttttt ccagcaccta gatcaataaa
     8461 cgcaccaaaa tttgtgattc ctgatacttt tccttgcagt tttgctccta cctcgattga
     8521 cataaaaaaa atgttcctcc taattatttc ccttttttat attcctgctt attttgcagt
     8581 tgtagaacta gttgattgat ctgtttcatt tgtcgagctg tctgtatttg attctaatat
     8641 aggataaact ttttcaccgt cttttgagta aaagaatttg cttctggcaa gtttagctac
     8701 atactcatca tctttcagca atgaaacatc ttggtgcagc tgatcaacgt ctgcgttgac
     8761 ttctttcatt tcagtatttg cttcgactct caattcgttc aattgatgca tcctttgcat
     8821 gtcatggaaa atctgcacac ctattatcgc gaaaatgacc aaagcaccaa caaaaattgc
     8881 agccagtctg cgtcttcgaa aaatcagttg acgttgttgt tgctgaaatt cagcatactt
     8941 tttctttgca tattccgtat ctagcgcagc gattttattg ctttttttcg tcacttggat
     9001 tcgctccaat ctttttgatt gtctttttca ttataccaat atcgtgaaag cttgtctaat
     9061 ctatttctct agtgtttctg tgcgattttc agaaataatt tcgtacattt tttgtgcatc
     9121 ttcttttttg gttgattcat gtaattccgt aattttcact tccaacgttt tgttgccaaa
     9181 ctggactttc aaaatatcgc ctactttgac atcactggaa gatttcgcca atttaccgtt
     9241 gatttgaatt cttcctttat cagctacttc ttttgctact gatcgtcttt tgatgattcg
     9301 ggaaattttt aaaaatttat ctaatcgcat atcttattcc tcacttttta atttttttat
     9361 ttaatcgcag gattttctta ccaaaaggca acattagcca ttctcgtact gtaaacagac
     9421 gaagacgaat cactgcataa atgaacacag aagccccgat cccaactcct atcacgctga
     9481 caaaaaaagc agtggaacga tgagttacag gaccaaatac tatattcaat acaccataat
     9541 acagcagcag tactactgtc atacaaagaa ggctgaacat caacttccgt ccaaaatgcc
     9601 gttctttcca aaaagcattt agctcttttg tttcaattcg aacaaaatac caaagcgtca
     9661 cacctaatcc taaaagggta gacaggcttg cacctgctgt tcctaataaa ccggtcagaa
     9721 aagaagtagt caatcctttt accaatagtc cccatcctgc gccttttaaa gaagggcgaa
     9781 acgagttctt gctttgagca atactttgat aagcttggat gactgcagtg aaagcaatgg
     9841 aaaaaacaaa taatacaagc gctgcatttc ccgcataatc tttaaacaac gcataattga
     9901 tataagggag cagcaaggct aatcccaaag acgcagctaa tgctaaagcg gtcgtcaatc
     9961 taagatagat tttggctgtc tgtaaaaact gacgatagat ccggttggtc aaatgacgag
    10021 tcaacgcagg caggaatgtc gcacttaatg cagtggcaat cactaaacct aattgtacta
    10081 aaggctgacc tcgatcatag accccttttg ctatcttcgc accgtattct gacagtccgt
    10141 aaacttccaa agcatttttt acaaagaaag agtcgatcag ctggaaaaag ataagcaatc
    10201 cactataaat cgaaaccagt ccgccttcta ccagaaaacg ccggatcaag ggctttgacg
    10261 gctgtttgaa caatggaaac cgtcggaagc ttaacgctcc tccatgaatc ttttgttcat
    10321 agtagtgaag gatcccatat gcgcataaac cgccaaaaac agctccactc attgctgccg
    10381 tccctgtttg gtacaccgtc catccaagct ttctaaaagc aaacgcacta cagacaatca
    10441 caaaaacacg aacgaactgc tcaactactt gagaaacagc agttggttcc atcaagaatc
    10501 ttccttggaa attccctcga taaaaagata gaccaggcat cagcaaaaag gtaaaggaaa
    10561 ctacttggat cagcggtttc aattgtatgt ctcccatcat ccaagcaatc cagctgctaa
    10621 atgcaaagac aaacaaccac aaaaagacac ctgtccaaaa tactaaggga taaagatttt
    10681 gcaaagcttg ctttcgctcg aaaggattct ttctttctgc aacgtacttt gatatgaact
    10741 gcggaagacc ggataaagct aaagtcattg ctatcccata aattggataa acttgctgat
    10801 aaacataaaa accttcgtcg ccgactaagt tttggagggg aattcggtaa agggcactta
    10861 atacttttgc tatgaaagaa gcaatcgtca atacaaatgc tccttgcatg acgcgccgca
    10921 tttctttatg atccaaatca ttgctccttc ctacgctgac agatattttt gctcacgcaa
    10981 cgctttgaca aacttttgga tttcctgaag ccatactgct tcttccattt ggttggggat
    11041 cgtcaagcgt atcaccatct gttctttttc aacgcctaga ctggctttta gttttgtcgc
    11101 tgtcagtgct tcgaataact gttccacact atagcgtttt gttccaactt tacttagagt
    11161 aaagactata tgcgcctcct gtttccttat cgtttctagg agcgctcgat cgccatccat
    11221 tttgatctgc ccaattgtca agagatgtgc tacttcttct ggatattctc caaaacggtc
    11281 gagcagatca tcttctagtt catctagcat ttcttgcgaa tcaagttcac gaattcgttt
    11341 atagatctcg attttctgcc gttgatctgc cacgtatgtt tcaggtagat acgcatcgac
    11401 accgaggtcg atctcaacag tggtcttctc cacttgacta ttctttcctt gtttacgaga
    11461 aacagcttcg cttagcattt gagaatacat atcgaaaccg actgcatcaa taaatccatg
    11521 ctgctgagca cctaagagat tacctgcacc gcgaatggaa agatcccgca tcgcaatctt
    11581 gaatccagat cccagttctg taaaatcttt gattgcctga agacgttttt cactgacttc
    11641 gttaaggatc ttctgctgct catacataaa gtaagcatat gcgacacggc tgcttcttcc
    11701 tacccgccct cgcaattggt ataacgtgga taatcccata taatcagcgt tctcaacgaa
    11761 caacgtattt gcgttcggga tatctactcc tgtttcaatg atggttgtag tgacaagaac
    11821 atcatattga ccttcaataa attcaaaaag cgtattttct aattgcgcct ctgtcatttg
    11881 cccatgagca taaccgatcc gtgcttccgg aacgagttct tggatttctt ctactttttg
    11941 ttcgatcgtt tctacccgat tatataaata gaatacttgt ccacctcgtc ccatttctcg
    12001 atggattgct tcgcgaatag ccccaggatt tttttccata acatacgttt ggatcggata
    12061 acgattcgca ggcggtgttt cgattactga aagatcccgc acgcctaaca tcgacatatg
    12121 caaagttctc ggaatcggtg ttgcagtcag agtcaaaaca tccacttgtg cacgtaactg
    12181 tttcaatcgt tccttatgtt ttacaccaaa acgttgttct tcgtcaatga tcagtagacc
    12241 caaatcgcta aattcaatat cttttgacaa aattcggtgg gttcctacaa caatatcaac
    12301 ttgacctgta cgcagttgtt cgatggtttc tttttgttgc tttcttgttc tgaaacgact
    12361 taatagcccg atatttacgg ggaaaccttc aaatctttcc agcatcgttt catagtgctg
    12421 ctgtgccaag atcgttgttg gaactaaaaa ggctacttgt ttgctttcct ttatcgcttt
    12481 aaaggccgct cgaagcgcta cttctgtttt cccataacca acatcaccta cgagtagtcg
    12541 atccattggt ttttcttttt ccatatcccg tttgatctcc gctgcacttc tcaactggtc
    12601 atcagtttca gagtagggga aggcattttc gaattctttt tggtaggcgt catcaggtcc
    12661 aaaagcatat cctttttctg actctcttgc ggcatataat ttgattagat cgtcagcgat
    12721 atcctcgatt tttgaagaga cttttcgctt ggttttcgtc cattcacttc cgcctagctt
    12781 attgatgcga ggcgctttgg cttcagaggc gacatatttt tggatcagat tcagttgagt
    12841 aacaggaata aacagtttgt cgtcgttttg atacaaaata gtcatatagt cttggtgaac
    12901 gccatctact tctagcgttt ccatcccaat gtatttcccg ataccatgat tggcatggac
    12961 aacgtaatct cctgctttta actcattata gcttttcaat cgctctgcgt ttgttactgt
    13021 ttgacgtctc gtttgttttt tcgtcgtttt ttggaaaatt tctttttctg tgatagctac
    13081 tattttttct tgaggaagtt caaaaccagc ctgcaaagct ccttcaacta attgtacctg
    13141 tcctctgatc aatgcatttt tttctgtttc tactactaat atgtcttcat ctcgaagcat
    13201 ttgttctgct ttgcgtattc tgtcctttgt cggaataaag atcacaacag tctgtttttg
    13261 cttgcgccaa cggtccattt ctgttttcag caatggcatt tgtccgaaga actgttgcat
    13321 cgtccgatat tgaaaattat ggattgcttg gaaacgaaga tttcccatcc ctttttggaa
    13381 gagtgagaaa aaggttgtgg tgaacttttc cttctgcacg attttttgta tatctgctga
    13441 aaatgtctgc tcagaaaaca cccgcaattc gctgattttt tgagtctgcc attctgccgc
    13501 ttccctttcg atttcccgat ttgtttccat gattcgctga taatcatcaa cgactagtaa
    13561 actatttgaa gggaaataat ccaagatcgt cacagattct tggtaaagaa gatctgagta
    13621 gaattttgct tcttctgtcg ggacacctgc ttgccagctt gaagtcagct gaccgaagta
    13681 atcttgcaaa aactcgcggt ctgcttcttc tgtggtcgca gcaagccgtt tttctagcat
    13741 tttttgcatt tgctctattc catgttctag atcctctggt ccgtagactt gttcactcgt
    13801 tggggaaacc cagatttctt ccgttttttc gattgagcgt tgtgtttctg cttcaaaata
    13861 ccgcatagaa tctatctcta catcaaatag ttctacacga acaggatagg ttgtcgttaa
    13921 cgggtaaata tcgataatgc ttcctcgaat actgaattct cccggttttg cgaccatctc
    13981 ttctcgtaca tagcccatca aagtcaattg gcgaggaaga ttttccacgt ctatctcatc
    14041 accaatatgc caattcaatt gcgcctgtag ccaagttttc ttattcggca gtcgtttgtg
    14101 caaagatgcg actggcaaca aatagatacc tgcttttttt tcttgaagtg cgttaagcgt
    14161 tgcaactctc tcgcctcgcg cttctggaga agaaaaggcc atttcagcag aaagcacttc
    14221 atccactgga aataaatgga tctgatcttg cggaatcaca tgttgcagat cttctgctaa
    14281 ctgattcgca taatacaaat ttggcatcag aataatcatg tttttatttg ttttcttaaa
    14341 cgctcgagca aatagaagtg ttttcgctga gcccgctaat cctgtgatta gttgtctagg
    14401 cattttttct tctaaattgc tgacccattc ttttacttgc ggatcttttc cgactaattg
    14461 aataatatcc attccttttc ctccttcctc aattgaatcg attcatcgca tccacgaacg
    14521 aatgcccttc tatgagatat tcgactgctt taacagattg acacatgctt tcttctatca
    14581 aaggctgatt ttctttgctg aaaggactaa gcacatgctg gactactgtt tttttccctt
    14641 ctggcctgcc gattcccacc ttgattcgat caaagacatt cgtattcaaa tgggaaataa
    14701 tacttttgat accgttatgc ccgccggcac ttcccttttg acggagccgg atttttccca
    14761 ctgccagatc caaatcatcg tagattacga ctaattcctc tggataaatc ccaaaataag
    14821 tcattagtgg gccgactgca cgaccagatt cattcatgaa tgtttgtggt ttgactaaaa
    14881 ggatcttttc tccattatga aaaaattcgg caacttctgc ttcgaacgga ttttttttga
    14941 aagtagcttg atgctcgcgt gcaattttat ccacgaccat aaagccgata ttgtgttttg
    15001 tatattggta tttggtacct gggtttccta acccgacgat cattttcatt tttgttttcc
    15061 acctaattct aaaaagcagc acaaaaggct ggactgctaa ttagcggtcc agtccatcga
    15121 gcttttttct tttttacact tgtcaatgca tagtataaca caaaacaagg cgtttcggct
    15181 aaaagagaat cgttaataaa tgataaagaa attaaaaatt tcttcaaagt ttaatgaaat
    15241 aattgatata taaaaatagt gaaataacgc acatgtaagc ttttacatat aatacaacct
    15301 tgttatctgt tatattttat agccaaatag cgtgacatgg ggcttgagaa atgttagcat
    15361 aagcatggga aaaaacaaat caatggaagg attgatacta aatgactgca gctgcagaaa
    15421 aaaaagatca tcaaaaagtt attcttgttg gagacggcgc cgtaggttct agctatgcct
    15481 ttgctctagt tactcaaaat atcgctcaag aagtaggtat tgtggatatc aatactgcta
    15541 aaactgaagg agacgcaatt gacttgtctc acgctttggc atttacttca cctaaaaaaa
    15601 tctattctgc tacttacgca gacgcacatg atgctgactt agttgttatt acagctggcg
    15661 caccacaaaa accaggtgaa actcgtttag acttagtaaa caaaaaccta aaaatcaacc
    15721 gcgatgttgt cacacaaatc gttgaatcag gatttaacgg tatcttttta gttgccgcta
    15781 acccagtaga tattttgact tactcaactt ggaaattctc aggtttccct aaagaacgcg
    15841 taatcggttc aggtacctct cttgactcag ctcgtttccg tcaagcactt gcggaattag
    15901 ttgacgttga tgcacgtaac gtccatgctt acatcttagg tgaacacgga gattcagaat
    15961 tcccagtttg gtcacatgca aatgttgccg gcttgcaaat ctatgaatgg gtaaaaaaca
    16021 acccagaaat cgatgaagaa gctatggtta acctattctt tggcgtacgt gacgctgctt
    16081 acacaattat cgagaaaaaa ggcgctactt tctatggtat cgcagtagca ttagcacgta
    16141 tcactcgtgc catcttagat gatgaaaatg ctgttcttcc tttatcagta ttcatgaatg
    16201 gcgaatatgg attgaacgac atctacattg gtgcacctgc tgttatcaac cgtcaaggta
    16261 tccaaaaagt aatcgaaatt cctttaagtg acagcgagca agaccgtatg gctgcttctg
    16321 ctaagcaatt aaaagaaatt ctagatgaag cttttgctaa actagacgct gaataatctt
    16381 tgaacatgac ggaaaacatc tattcggtgt tttccgtttt tttattttat cacccttatg
    16441 gtttctaaat tttttcataa agtagagata accttcgtct tttcctctaa attatgctat
    16501 catgagaccg ttccacccta aacttttact taaggagttg tcgatatgac aagatcttcg
    16561 catcgaaaaa aatcgaaaca tgttgctttg ctttcagttc ttggtgttct cttagtgctg
    16621 atcagctttt atacctatcg cagtacatac tacacaaagc attttttgcc gaatacagaa
    16681 ataaatggaa taaatgtcag caatctaaca gtcgaaaaag caaatgaaaa attaaaagaa
    16741 gcttattctg ataaaaaact ggctattaaa gaagatggaa aagtctggca ggaagtacca
    16801 aaatccgagc tcggctacaa agatgatttc acttcggagc tttctccgat cctaaatgaa
    16861 caaaacggat ggacttgggg aatgacttat gtctcagcgg cagaaaaaca agaaatagat
    16921 cctgtcagtc aaaaccacca aaaactagac actaccgttc agacattgac aactaagctg
    16981 actgatttga acaaagaccg gacacctact actgatgcaa cgatcgaaaa agcaggtgac
    17041 tctttcgtta tcaagcctga agtcaatgga aatacgatcg acgtcgatgc tgcggtcaaa
    17101 caattaaaaa gtgcggtaaa ctctggtaaa gatacaattg aattgactga attcaaagaa
    17161 aaaccaaaag taacatctga agacagctca ctgaaagaac aactcgcatc catgaatgct
    17221 atcgcaaatg taaaagctac ttacagtata aacggtgaga ctttccagat cccatcaagt
    17281 gatattatga gttggctgac ctacaatgat ggaaaagtcg atttagatac agaacaagta
    17341 cgtcaatatg taacagattt aggcacaaag tataacacaa gtacgaatga cacaaaattt
    17401 aaaagtacaa aaagaggaga agttacggtc ccagttggca cttacagttg gacgatccaa
    17461 acagacagtg agacagaagc attgaaaaaa gcgattctgg caggccaaga ttttactcgt
    17521 tcacccattg tgcaaggtgg cacaaccgct gatcatccat tgatcgaaga tacgtatatc
    17581 gaagttgatc tggagaacca gcatatgtgg tattacaaag acggaaaagt ggcccttgaa
    17641 acagatatcg tttccggaaa gccaactaca cctacacctg ctggagtctt ctatgtttgg
    17701 aataaagaag aagatgcaac tttaaaagga acaaatgacg atggaacacc ttacgagagt
    17761 ccggtaaact actggatgcc gattgactgg acaggtgttg gtatccacga ttcagactgg
    17821 caacctgaat acggtggaga cttatggaaa acccgcggtt cacacggctg catcaacacc
    17881 ccaccaagtg taatgaagga attattcggg atggtcgaaa aaggaacgcc tgtattggtc
    17941 ttct
//
LOCUS       DACEEZ010000055        17746 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20313_2017
            SAMN12639966-rid9879223.denovo.055, whole genome shotgun sequence.
ACCESSION   DACEEZ010000055 DACEEZ010000000
VERSION     DACEEZ010000055.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639966
            Sequence Read Archive: SRR10023158
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 17746)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17746)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741683.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741683.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:44:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,016
            CDSs (total)                      :: 2,962
            Genes (coding)                    :: 2,889
            CDSs (with protein)               :: 2,889
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 36 of 73
            Pseudo Genes (incomplete)         :: 29 of 73
            Pseudo Genes (internal stop)      :: 18 of 73
            Pseudo Genes (multiple problems)  :: 8 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17746
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639966-rid9879223.denovo.055"
                     /isolate="E20313_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            <1..356
                     /locus_tag="IWO89_001881"
     CDS             <1..356
                     /locus_tag="IWO89_001881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HAQ0456612.1"
                     /translation="EIVIKQKVELYPNMCVTNFTESEQWDTVIEGNDDLLEKYMSGKS
                     LEALELEQEESIRFQNCSLFPLYHGSAKSNIGIDNLIEVITNKFYSSTHRGPSELCGN
                     VFKIEYTKKRQRLAY"
     gene            531..827
                     /locus_tag="IWO89_001882"
     CDS             531..827
                     /locus_tag="IWO89_001882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0456613.1"
                     /translation="MQIGVLKWLQFTKQKGVHFPMQFLEPKNKNAKSVDWEISEQVRV
                     IVKQYAEYAERTESEAVDEFLLNILDDKKFIEWIANKRSNKRIVEKMGIKDRVG"
     gene            834..2786
                     /locus_tag="IWO89_001883"
     CDS             834..2786
                     /locus_tag="IWO89_001883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017431446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insertion element protein"
                     /protein_id="HAQ0456614.1"
                     /translation="MTKLKRLATKEDEIVDVKIPISNEELKDRAKQYDLLTPKRFATR
                     YNKMLFLPTSFKWNGSEYPIQYNYCINPFCCNFGKEQHKFKDVKGKPSRYKMTGSSKD
                     KGHKGMYCNDNPIGRGVSQNCTVTPLSNWSVVEEIKRLIEINSIQDVEPDYQFHKEGC
                     SEEESTPFNEPKQFYKRGKSRGKSQRYQCKACKKFTNVLPKREETTTYHQQKNTILPM
                     FAKMVVGRVSVSRTCDILGIGVGTYYHKLEWLYRRCLEFLERYETQPLQTKKFNEMWL
                     NTDKMHYYLNNVRKKGQGSKKYTGFEDLNMQTYIVVSAEVLSRYVFRSDVAYDWNISM
                     DELNEDTRKFKEDHLNTFSRKNDRLDWSYYPQEPSANDSENRNAYLHELGKITNRSRF
                     VDGLNVDAPYTTTAHYWLIKQMVNADEWRMISDDDFSIRNAFYRVFTKELRLSDAHHF
                     ICQVNKTKSRKQCLKEFGQAKAELLDWGDIRGFKTKFLRTLASHYLTELLTSHQFHEE
                     AISKDGERYRKYADNPIKHPLATKDKGFYSVDCRTDLSALEPNEIAKMLLNVNDHSTN
                     SFIQQIRRYISSLERPLTTARGDKKSYIYANFNPKYAQFAITILRTYYNFCRPFKSAD
                     KKVLTPAQRLGITDKQFDWKDIIYFK"
     gene            complement(2858..3355)
                     /gene="dfrG"
                     /locus_tag="IWO89_001884"
     CDS             complement(2858..3355)
                     /gene="dfrG"
                     /locus_tag="IWO89_001884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000868795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trimethoprim-resistant dihydrofolate reductase
                     DfrG"
                     /protein_id="HAQ0456615.1"
                     /translation="MKVSLIAAMDKNRVIGKENDIPWRIPKDWEYVKNTTKGHPIILG
                     RKNLESIGRALPDRRNIILTRDKGFTFNGCEIVHSIEDVFELCKNEEEIFIFGGEQIY
                     NLFFPYVEKMYITKIHHEFEGDTFFPEVNYEEWNEVFAQKGIKNDKNPYNYYFHVYER
                     KNLLS"
     gene            3717..4757
                     /locus_tag="IWO89_001885"
     CDS             3717..4757
                     /locus_tag="IWO89_001885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000288739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HAQ0456616.1"
                     /translation="MYTSINGELCKIDRAYSGEIVILQNEFLKLNSVLGDTKLLPQRK
                     KIENPHPLLQTTVEPSKPEQREMLLDALLEISDSDPLLRYYVDSTTHEIILSFLGKVQ
                     MEVISALLQEKYHVEIELKEPTVIYMERPLKNAEYTIHIEVPPNPFWASIGLSVSPLP
                     LGSGMQYESSVSLGYLNQSFQNAVMEGIRYGCEQGLYGWNVTDCKICFKYGLYYSPVS
                     TPADFRMLTPIVLEQAFRKAGTELLEPYLSFKVYAPQEYLSRAYNDAPKYCANIVNTQ
                     LKNNEVIIIGEIPARCIQDYRNDLTFFTNGLSVCLAELKGYQVTTGEPVCQTRRLNSR
                     IDKVRYMFNKIT"
     gene            4855..5043
                     /locus_tag="IWO89_001886"
     CDS             4855..5043
                     /locus_tag="IWO89_001886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011988843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HAQ0456617.1"
                     /translation="MINYKWILCPICGNKTRLRIRVDTILKNFPLYCPKCKNETLINV
                     QKMNIITIKEPDAKTQSR"
     gene            complement(5101..5454)
                     /locus_tag="IWO89_001887"
     CDS             complement(5101..5454)
                     /locus_tag="IWO89_001887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001227349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAQ0456618.1"
                     /translation="MRKKEDKYDFRAVGLAIKEARMKRGLTREQVGTMIEIDPRYLTN
                     IENKGQHPSTQVLYDLVSLLHVSIDEFFLPTDNLIKSTRRLQVEKYMDSFTDKELSLM
                     EALAKGINEARNIEG"
     gene            5985..6407
                     /locus_tag="IWO89_001888"
     CDS             5985..6407
                     /locus_tag="IWO89_001888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000804881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HAQ0456619.1"
                     /translation="MKPSSFQETIENQFDYICKQVIEDERKDYFKHLDRLSAKEVSFS
                     DIDNYLVNSFSTVDTYVTDFQLFKLFGLRINVESDLLSEALRHLSEKKRNIILLHYFM
                     DMSDVEIAELLNLNRSTVYRHRISGLAMIKEFMKECKE"
     gene            6404..6634
                     /locus_tag="IWO89_001889"
     CDS             6404..6634
                     /locus_tag="IWO89_001889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002411750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAQ0456620.1"
                     /translation="MKTAYPRVPFPLIVKATDGDVEAINQIVKHYRGYTSKRSLRRMT
                     DEYGNSHMVIDETLRGRMETKLISKILSFEIK"
     gene            7132..7332
                     /locus_tag="IWO89_001890"
     CDS             7132..7332
                     /locus_tag="IWO89_001890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008475635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3173 domain-containing protein"
                     /protein_id="HAQ0456621.1"
                     /translation="MITVTKKDLVELGYGTSFSADIIKKAKELMITKGHIYYQSRKLD
                     RVPREAVEEILGIRFSNENRSE"
     gene            7359..8552
                     /locus_tag="IWO89_001891"
     CDS             7359..8552
                     /locus_tag="IWO89_001891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002388733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HAQ0456622.1"
                     /translation="MVKDPIKKAKNGTYYFRANLGYDSKGKKIQKYRSGFTTKKEARE
                     EYSKLLLMKPEELSENKDKMTFKHYIFEIFLPWYKTQVKLRTYENRLPTIKKHFSYFD
                     KMAVSDIEPIDVQNWQLKLSKKCKSSYVRAVQGLFSVAMDRAIVLGITTTNPSKIIGN
                     VKKQKSKIEFWTKEEFEKVISLIYKEDYYQHFLFISLWFLFMTGMRIGEATAIQWEDI
                     DFDSGVLTINKTLYYKNQSNYRFVEPKTKASVRHIVLDKCTLTYLSEWKEVQQNLIQT
                     DFVMSYNGIPTQKHTLSYAIERYAKLAGIHRIRIHGLRHSHASLLISMGENPLIIKDR
                     LGHEDIETTLGTYGHLYPNSNFEVANKLNGAISFKEAKQNYDTSPKNQFTVDYLRNKE
                     MKKVQ"
     gene            complement(8620..10185)
                     /gene="guaA"
                     /locus_tag="IWO89_001892"
     CDS             complement(8620..10185)
                     /gene="guaA"
                     /locus_tag="IWO89_001892"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HAQ0456623.1"
                     /translation="MTNVADMTNVEKIIVLDYGSQYNQLITRRIRDLGVFSELLSHRI
                     TAEEVKAMNPKGIILSGGPNSVYDENAFGIDPEIFNLGIPVLGICYGMQLITYKLGGS
                     VEPAKNREYGKALLEVTSDQAVLFKETPKQQTVWMSHGDLVTKVPEGFETVGTSKDCP
                     IASIQDTTRNFYGIQFHAEVRHSEYGIELLRHFAFDVCHCEGNWTMDNFIDMQVEKIR
                     EQVGDKKVLLGLSGGVDSSVVGVLLQKAIGDQLTSIFVDHGLLRKGEAEQVMESLGGK
                     FGLNIIKVDARDRFLDKLKGVSDPEQKRKIIGNEFVYVFDDEATKLAGEEGVSFLAQG
                     TLYTDVIESGTETAQTIKSHHNVGGLPEDMQFELIEPLNTLFKDEVRELGTQLGMPDS
                     IVWRQPFPGPGLGIRVLGEITEDKLEIVRESDAILREEIAAAGLDRDIWQYFTVLPGI
                     RSVGVMGDGRTYDYTVGIRAVTSIDGMTADFARIPWEVLQKISVRIVNEVAHVNRIVY
                     DITSKPPATVEWE"
     gene            complement(10244..11167)
                     /locus_tag="IWO89_001893"
     CDS             complement(10244..11167)
                     /locus_tag="IWO89_001893"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I pantothenate kinase"
                     /protein_id="HAQ0456624.1"
                     /translation="MNDPMNYYRVAREEWREFYRNGQAPLTQDELDNIKSLNDRISMQ
                     DVRDIYVPLAHLIYLYMKEFESLFLSKGLFLHKYVEVPPFIIGIAGSVAVGKSTTARL
                     LQTILSRTFKRRNVQLITTDGFLYPNRILKERGIMDRKGFPESYDMEMLIDFLNRVKS
                     GQEEIKVPVYSHDVYDIIPDKYELIQQPDILIVEGINTLQLPANQQIYVSDFFDFSVY
                     VDADPVLIEKWYLDRFGALLDTAFQNPENYYYRFAIGDREEAFKMAKDVWKNVNLKNL
                     QEYILPTRGRADIILHKTENHIIDSIFMRKY"
     gene            11363..11968
                     /locus_tag="IWO89_001894"
     CDS             11363..11968
                     /locus_tag="IWO89_001894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HAQ0456625.1"
                     /translation="MTNHYYSERPDTAHDFEQWAFELKGRTFQFVTDSGVFSRETVDY
                     GSRVLIDAFDWEELPDEGKILDVGCGYGPIGLALAFATQRFVEMVDINERAVSLAQGN
                     AKRNQIEHVDIHQSNIYEAVHEETYAAIVSNPPIRAGKKVVHEILTGAYPRLKKGGTL
                     TIVIQKKQGAPSAQKKMEETFGNAEIVTKDKGYYIIKSVKE"
     gene            12276..13292
                     /locus_tag="IWO89_001895"
     CDS             12276..13292
                     /locus_tag="IWO89_001895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family transcriptional regulator"
                     /protein_id="HAQ0456626.1"
                     /translation="MKLTIRDIAEMAGVSVTTVSQILNNKGSRFSDKTRKKVLSIVNE
                     HHYKPDYFASNVINRHSKTIGMIVPDVTDFFFSKLIEGVESYLNPLGYVIMLCNSRHS
                     QENEIKYLQELSHRSVDGILLATPNILPEKYALDSGFYQKMPIILIDRGLNRRDNGRL
                     IVKEYEGAYQAVSLFIENGHTKIGMLKETTGYYQLEERFNGYRHALKDHGLPFNGKFV
                     EHGELTVQGGYTASKKLLKHKDITAIFCGNDAMAIGCYQAIDELGKKIPEDISVIGFD
                     GLKLSEYMIPQLTTVQQPSFDIGFYAARFLIDTIEFPQRKVPNKVFETKLIIRESVKV
                     LTKG"
     gene            13433..14734
                     /locus_tag="IWO89_001896"
     CDS             13433..14734
                     /locus_tag="IWO89_001896"
                     /EC_number="2.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine-nucleoside phosphorylase"
                     /protein_id="HAQ0456627.1"
                     /translation="MRMVDLIEKKRDGNELSKEEIEYIVTNYTNGKIPDYQVSALLMA
                     IFYQDMTNEEITNLTLAIANSGDVIDLSSLEGIKVDKHSTGGVGDTTTLILAPLVASV
                     GVTVAKMSGRGLGYTGGTLDKLEAIPGFQIELSDEEFVRIVNESKVAVIGQSGNLAPA
                     DKKLYALRDVTATVDSLPLIASSIMSKKIAAGADAIVLDVTTGDGAFMKNIEDARRLA
                     KTMTSIGKLANRETVAVISDMSEPLGEAIGNSLEVVEAIETLQGNGPEDLVEMCYALG
                     SQMVVLAGKAKTTDEARTLLQEALESGKALAKFKEMIQNQGGDPAIVEHPERILTARY
                     TMELPAKQSGVVSKIVANELGIAAMMLGAGRKTKEEDIDHAVGLKLHKKIGDTVTKGE
                     SLLTIYSNDEEITSVIELLYKNIEIGESAMKPTLIHDIITE"
     gene            14780..15442
                     /gene="deoC"
                     /locus_tag="IWO89_001897"
     CDS             14780..15442
                     /gene="deoC"
                     /locus_tag="IWO89_001897"
                     /EC_number="4.1.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribose-phosphate aldolase"
                     /protein_id="HAQ0456628.1"
                     /translation="MELNRMIDHTILKADASKEAVMQIIEEAKKYHFYSVCINPAWVA
                     LAKEQLQGEPVAVCTVIGFPLGANTPETKAFETTDAINNGADEVDMVINIGALKSKDD
                     QQVQKDIEAVVEAAKDRALVKVIIETSLLDREEIIRACEIAKAAGADFVKTSTGFSTG
                     GAKVEDVRLMRETVGPEMGVKASGGIHNEEEAMAMIEAGATRIGTSAGVAIVSGSTGE
                     GY"
     gene            15463..15861
                     /locus_tag="IWO89_001898"
     CDS             15463..15861
                     /locus_tag="IWO89_001898"
                     /EC_number="3.5.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine deaminase"
                     /protein_id="HAQ0456629.1"
                     /translation="MAELKQEWINTAIEALDKAYVPYSHFPVGACLVTESGKIYQGIN
                     IENASFGLTNCAERTAFFKAVSEGEKTFTHLVVAGHTPDPISPCGACRQVMAEFCSPD
                     MPVTLVGDNGVTKATTVRALLPYAFTEKDL"
     gene            15927..17009
                     /locus_tag="IWO89_001899"
     CDS             15927..17009
                     /locus_tag="IWO89_001899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BMP family ABC transporter substrate-binding
                     protein"
                     /protein_id="HAQ0456630.1"
                     /translation="MKKAKLFGLGAVALAAGLFLGACGNNGSTDSSGGKSSSDTTTAA
                     LITDTGGVDDRSFNQSAWEGLEKWGKDQGLSRGNDGFQYFQSSNESDYIPNIDQALNA
                     GFKTIFGIGYKLKPAIQEQATNNTGTNFVIIDDVIDGLDNVVSATFKDNEASYLAGVA
                     AAYTTETNVVGFIGGVKGEVIDRFDAGFKAGVDAGAKELGKEIKVLNQYAGDFSAPDK
                     GRSIAQGMYAQNADIIFHASGGTGNGVFQEAKSLNESGDKKVWVIGVDRDQSDEGEYT
                     LNGEKKNFTLTSTLKAVGTVVEDLAQKSADGKFPGGEHTVYGLKEDGVGLTEGQLSDE
                     AKKAVDEAKEKIISGDVKVPETPEEN"
     gene            17221..>17746
                     /locus_tag="IWO89_001900"
     CDS             17221..>17746
                     /locus_tag="IWO89_001900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAQ0456631.1"
                     /translation="MVQENIVIEMQNITKEFGTFKANDNINLQVKAGEIHALLGENGA
                     GKSTLMNVLSGLLEPTSGKILMRGKEVKITSPTKANQLGIGMVHQHFMLVDAFTVTEN
                     IVLGSEPSRAGMLDHKKARKEIQKVSEQYGLSVNPDAYVRDISVGMEQRVEILKTLYR
                     GADVLIFDEPTAVLT"
BASE COUNT         5948 a         3124 c         3546 g         5128 t
ORIGIN      
        1 ccgaaattgt aatcaaacag aaggtagaac tgtatcctaa tatgtgtgtg acgaacttta
       61 ccgaatctga acaatgggat acggtaatag agggaaacga tgacctttta gagaaatata
      121 tgtccggtaa atcattagaa gcattggaac tcgaacaaga ggaaagcata agatttcaga
      181 attgttctct gttccctctt tatcatggaa gtgcaaaaag taatataggg attgataacc
      241 ttatagaagt tattactaat aaattttatt catcaacaca tcgaggtccg tctgaacttt
      301 gcggaaatgt tttcaaaatt gaatatacaa aaaaaagaca acgtcttgca tattaagtag
      361 ttcagttttg gagtacaaaa agtgaaaatg taaacctaaa tgagtatcaa attgcaactg
      421 aatttgatac tcgtttttta ttgttaaatc aacgtttgtt gttaatttac accacaaaat
      481 gtaagttaaa atgtatataa aaatgcgaac gtgaaataat tattgtagga atgcaaattg
      541 gtgtgttaaa atggttacaa tttactaagc aaaaaggggt gcattttcct atgcaatttc
      601 ttgagccaaa aaataagaac gctaagtctg tggattggga aatatcagag caagtaaggg
      661 tgatagttaa gcaatacgct gaatatgcag agcgtactga aagcgaagca gtggatgaat
      721 tcttattgaa cattttagac gataagaagt ttattgaatg gattgccaac aagcgaagta
      781 ataagcggat tgttgaaaaa atgggtatca aagatagagt gggttgatag acaatgacca
      841 agcttaaaag attagccacg aaggaagatg aaattgtaga tgttaaaatt ccgatttcaa
      901 acgaagaatt aaaagatagg gctaaacaat atgatttatt aactccaaaa cgatttgcaa
      961 caaggtataa caaaatgttg ttcttgccaa cgtcttttaa atggaacggt tctgaatatc
     1021 ccattcaata taattactgc atcaatccgt tttgttgtaa ttttgggaaa gaacagcaca
     1081 aattcaaaga tgtaaaaggg aaaccaagtc gatataaaat gactggtagt tccaaagata
     1141 aaggtcataa aggtatgtac tgcaatgaca atcctattgg cagaggtgta tcgcaaaact
     1201 gtacagtaac ccctttatct aattggtctg ttgttgaaga aattaaacgc cttatagaaa
     1261 tcaatagcat ccaagatgtt gagccagatt atcaatttca taaagaaggc tgctcggaag
     1321 aagaatcaac cccattcaat gagccgaagc aattttacaa aagggggaaa agtaggggca
     1381 aatctcaacg ctatcaatgt aaggcgtgta agaagttcac taatgttcta ccaaaaagag
     1441 aagaaaccac tacttatcac caacaaaaga acaccattct tccaatgttc gctaagatgg
     1501 ttgttggaag ggtttcagtc agtcgtactt gtgatatttt aggaattgga gttggaacct
     1561 actatcataa gctggagtgg ctgtataggc gttgtttgga gtttttagaa cgatatgaga
     1621 cacaacccct tcaaacaaag aaatttaacg agatgtggct taatacggac aagatgcact
     1681 actacctaaa taacgtccgt aagaaaggac aaggttcaaa gaaatatacg gggtttgaag
     1741 accttaatat gcaaacgtat atagttgtat ctgctgaggt cttatcaaga tatgtgttcc
     1801 gttctgatgt tgcctatgat tggaatattt caatggatga attaaatgaa gatacaagaa
     1861 aattcaaaga agaccatctc aatactttta gtcgtaaaaa tgataggtta gattggtctt
     1921 attatccaca agaaccatcc gctaacgata gcgaaaatag gaatgcttat cttcacgaat
     1981 tagggaaaat tactaatagg agtagatttg tcgatggttt aaatgttgat gctccttata
     2041 ctacaactgc tcactattgg ttaattaaac aaatggttaa tgccgacgaa tggcgaatga
     2101 ttagtgatga tgacttctct attagaaacg ccttttatag agtattcacg aaagaattaa
     2161 ggttgagcga tgcacatcat tttatttgcc aagttaacaa gacgaaaagc agaaaacaat
     2221 gcttaaaaga attcggacaa gcaaaagcag aactgctcga ttggggagat atcagaggat
     2281 tcaaaacaaa gtttctacgg actttggcat ctcattatct aactgaatta ctaacaagtc
     2341 atcaattcca cgaagaggct attagtaaag atggagaaag atatcgcaaa tatgccgata
     2401 accctatcaa acatccatta gccactaagg ataaaggatt ttattcagtt gattgtagaa
     2461 cggatttatc tgctctggaa ccaaacgaaa tcgcaaagat gctattaaat gttaatgacc
     2521 attcgaccaa tagctttatt caacagataa gacgttatat atcatctctt gaaagaccat
     2581 taacaacagc tcgtggtgat aagaagagct atatttacgc taacttcaat ccgaagtatg
     2641 ctcaatttgc gattacaatt ctaagaactt actacaactt ttgtcgccct tttaaatcag
     2701 cggataaaaa ggttttgaca cctgctcaaa gattagggat tacagataaa caatttgatt
     2761 ggaaagatat aatttatttc aaatgacaaa aagagcttgg agtaatccca agctcttgtg
     2821 ctttatcttt actgttcttc ttcaattaac gcacccgtta actcaataag ttttttcttt
     2881 catatacatg aaaatagtag ttatacggat ttttatcatt ctttatccct ttttgggcaa
     2941 atacctcatt ccattcctca taattcactt ctggaaaaaa agtatctcct tcgaattcat
     3001 gatgtatttt tgtgatgtac attttctcaa cataagggaa aaacaaatta taaatctgtt
     3061 ctcctccgaa aataaaaatt tcttcttcgt ttttacataa ctcaaaaaca tcttctattg
     3121 aatgaacaat ttcacaacca ttaaaggtaa accccttatc tctcgtcaga ataatatttc
     3181 ttctgtcagg taaggctctt ccgattgatt caaggttctt cctacctaat attatcggat
     3241 gtccctttgt agtattttta acatattccc agtccttggg aatcctccaa ggaatgtcat
     3301 tctctttgcc aatcactcta ttcttatcca tcgcagcaat caaagaaact ttcatttata
     3361 acacagcctt tttaactaat ttaccatcta atttcttatt tctatcaaaa aaccttttct
     3421 cattatcctg cccgttagtt gaataagcaa ttttttctat aagttatatt atcataatac
     3481 cacaaaatca caaattttca ttcaattatc attttcaatt gcattttata aatcattttg
     3541 tatgtaattt ggtatgtaag ttaaaatgaa aaaagtagtc cattttgaag tacattttgt
     3601 cattttgtac tccaaaactg aactacttac ttgcatatat acgcctttat agtggagtac
     3661 tacatttacg agattcggtt agagtatcag aaaaagaaaa aataaaagtt acagaaatgt
     3721 atacttcaat aaatggtgaa ttatgtaaga ttgatagagc ttattctgga gaaattgtta
     3781 ttttgcaaaa tgagtttttg aagttaaata gtgttcttgg agatacaaaa ctattgccac
     3841 agagaaaaaa gattgaaaat ccgcaccctc tactacaaac aactgttgaa ccgagtaaac
     3901 ctgaacagag agaaatgttg cttgatgccc ttttggaaat ctcagatagt gatccgcttc
     3961 tacgatatta cgtggattct acgacacatg aaattatact ttctttctta gggaaagtac
     4021 aaatggaagt gattagtgca ctgttgcaag aaaagtatca tgtggagata gaactaaaag
     4081 agcctacagt catttatatg gagagaccgt taaaaaatgc agaatatacc attcacatcg
     4141 aagtgccgcc aaatcctttc tgggcttcca ttggtttatc tgtatcaccg cttccgttgg
     4201 gaagtggaat gcagtatgag agctcggttt ctcttggata cttaaatcaa tcatttcaaa
     4261 atgcagttat ggaagggata cgctatggtt gcgaacaagg attatatggt tggaatgtga
     4321 cggattgtaa aatctgtttt aagtacggtt tatactatag ccctgttagt actccagcag
     4381 attttcggat gcttactcct attgtactgg agcaagcctt tagaaaagct ggaacagaat
     4441 tgttagagcc atatcttagt tttaaagttt atgcaccaca ggaatatctt tcacgggcat
     4501 ataacgatgc tcccaaatat tgtgcaaata tcgtaaatac tcaactgaaa aataatgagg
     4561 tcattattat tggagaaatt cctgctcgat gtattcaaga ttatcgcaat gatttaactt
     4621 tttttacaaa tgggcttagt gtttgtttag cagagctaaa aggatatcag gttaccactg
     4681 gcgaacctgt ttgccagacc cgtcgtctaa atagtcggat agataaagta agatatatgt
     4741 tcaataaaat aacttagtgc gttttatgtt gttatataaa tatggtttct tattaaataa
     4801 gatgaaatat tctttaatat agatttgaat taaagtggaa aggaggagat tgttattata
     4861 aactacaagt ggatattgtg tcctatttgt ggaaataaaa caagactacg aatacgagtg
     4921 gatactatac ttaaaaattt ccctttatac tgccccaaat gtaagaacga aactttaatt
     4981 aatgttcaaa aaatgaatat aataacaatc aaagagccag acgccaagac gcagagccga
     5041 taatttgaga aatgaaactc tcatcttatc ggctcttttt gtttatctga attttactga
     5101 ctagccttca atatttcttg cttcattgat accttttgct agtgcttcca ttaaggatag
     5161 ttctttgtct gtaaagctat ccatgtattt ttctacctgt aaccttcgag tgcttttaat
     5221 caaattatct gtaggtaaga aaaattcatc aatagataca tggagtaatg atacaagatc
     5281 atacagcact tgtgtacttg ggtgttgtcc tttgttttct atatttgtta aataacgtgg
     5341 gtcaatttca atcattgttc ccacttgttc acgagtgaga ccccgtttca ttcgagcctc
     5401 tttaatcgct aaaccaacgg ctctaaaatc gtatttatct tcttttttac gcatactaaa
     5461 tcacctctat acattttact gttcctatat aaccataaac aggtatagaa aaacatgtta
     5521 tatagtttat gagttcctat agaacactgt ggaatttatt aatgcgatga atccaatttc
     5581 aaatagaaag gtcgctgatt ttatttacct atacaacttt ataaaattta gtcatactcc
     5641 tagaaagtag ataaaattgg cgacttaatg gagggaaaag tcgccatgag aaagataatc
     5701 tgtcatctag ttctcttaat taagttctta tcaaaaaaac ttatccacct gtactggata
     5761 gatatgacca tcaatgtggt tttgcagatt acatagatag aaaaatcaaa gcatatgaac
     5821 agagaaaaca atctgtttat gtgctttttt ggtcacttaa taaatatctt ttaggatttc
     5881 tcttcattta tgcaacaaat cccccgttcg tgtcggggtt tagtatgaag ggagataaag
     5941 aagcacatgc cactcccttt cacgacagaa agggggtgaa aagcatgaaa ccatcttctt
     6001 ttcaagaaac catagaaaat cagtttgact atatctgcaa acaagttatt gaagatgaac
     6061 gaaaagatta cttcaaacat ctagacagac tatcagcaaa agaagtctcg ttttctgata
     6121 tagataacta ccttgtcaac tctttttcta ctgttgatac gtacgtcact gatttccaac
     6181 tatttaaatt atttggatta agaatcaatg ttgaaagtga tttattaagt gaagccctgc
     6241 gtcatctttc agagaaaaaa cggaacatta ttttacttca ttactttatg gatatgagtg
     6301 atgtggagat tgcggagtta ctaaatttga atcgttcgac tgtttatcgt catcgaataa
     6361 gtggactagc catgattaaa gaattcatga aggagtgtaa agaatgaaaa cagcttatcc
     6421 aagggttccc ttccccctca ttgtaaaggc gacagatggc gacgtggaag caattaatca
     6481 gattgttaaa cattatcgag ggtatacctc aaaacgttca ttacgtcgca tgacagacga
     6541 atatggaaat tctcatatgg ttattgatga aaccctacgt ggcagaatgg aaacgaaact
     6601 catctctaag attttatcgt tcgaaattaa atagtcccat tttttcgtgg aagcgtgtcg
     6661 aatcgtatcc acgcttcccg atttgggagg tttgttattc catcaaagca tattgtgtgt
     6721 tcagtaggtt ttgataggct aacaagccaa acgttctttg acaaccaaat aaaggcaatc
     6781 gaatacgttt cggtaagaaa agagctgatg aactaacacg ccatgaccta caaaattagt
     6841 gagcgattca tgttagtaat tcgaaagtaa tctttagcag gattgctgcc atgactttct
     6901 tatcgtgata atgatactcc cgtatagcca tagtccgagc gttagaagcg tcacaggcaa
     6961 tgggtacggc tacatgagaa ccatgcagaa gtgaaattcc tgtgagcttt gctagagctg
     7021 ttcgattgct aagatattct tctcttgtca gtgtattatt ataaaactat ttgatataat
     7081 tataggtgaa tattggagtt ttcctagcct ataattggga aaggagtaat aatgataacg
     7141 gtcacaaaaa aggatttagt tgagctagga tatggaactt ctttttcagc agacatcata
     7201 aaaaaagcaa aagaattgat gattacaaaa gggcatatct actatcaatc tcgaaagcta
     7261 gatagagtcc ctcgtgaagc tgttgaagaa attctaggaa ttcggttttc aaatgaaaac
     7321 aggtctgagt agtttttgca cagtaaggag taaaaactat ggttaaagac ccaataaaaa
     7381 aagcaaagaa tggaacctat tattttcgtg caaatctagg ttatgattct aaagggaaaa
     7441 aaattcaaaa atatcgtagt ggattcacta ctaaaaaaga agcaagagaa gaatattcga
     7501 aactgctatt gatgaaacca gaagaattaa gtgagaataa ggataaaatg acgtttaaac
     7561 attatatctt tgaaattttc cttccatggt ataagacaca ggtaaagctc agaacatatg
     7621 aaaatcgctt accaactatt aagaagcatt tctcttattt tgataaaatg gctgtttccg
     7681 atattgagcc tattgatgtg caaaattggc aactaaaatt atcaaagaag tgtaagtctt
     7741 cttatgtaag agctgttcaa ggactatttt cagttgctat ggatcgagca attgtccttg
     7801 ggataaccac cacaaatcca tctaagatta ttggcaatgt gaaaaagcaa aagtctaaaa
     7861 ttgaattttg gacaaaagaa gaatttgaaa aagtaatctc tctaatttac aaagaagatt
     7921 actatcaaca ctttttattt atctctctgt ggtttttatt tatgactgga atgcgaattg
     7981 gtgaagctac tgccattcaa tgggaagata ttgattttga ctcaggagta ctaaccatca
     8041 acaaaacact ctattacaag aatcaatcga attatagatt tgttgaacca aaaacaaagg
     8101 cgagcgttcg tcatattgta ttagataaat gcacattaac ttatttatcc gaatggaagg
     8161 aagtacaaca aaatctaatt cagacagact ttgtaatgag ctataacggt attcctaccc
     8221 aaaaacatac actatcatat gctattgaac gctatgctaa gctggctggt atccatcgaa
     8281 tcagaatcca tgggttacgg cattctcatg cttccttgtt aatcagtatg ggagaaaatc
     8341 cacttatcat taaagatcgt ctaggacacg aagatattga gacgacttta ggaacgtatg
     8401 gacatcttta tcctaacagt aatttcgagg tagctaacaa attaaatgga gccatttcct
     8461 ttaaagaagc aaaacaaaat tatgatactt caccaaagaa tcaatttact gtagactact
     8521 tgcgaaacaa agaaatgaaa aaagtgcaat aaaagtgcag taaaaagata agaagagcca
     8581 gaaacccttg gtataagagg atttctagct ctcctacaac tattcccact ccactgttgc
     8641 tggtggttta gaagtgatgt catacacgat acggttgacg tgggctactt cgtttacgat
     8701 acgtacagag attttttgca agacttccca tgggatacgg gcaaagtcag cggtcattcc
     8761 gtcgattgaa gttacagcac ggataccgac tgtgtagtcg taagtacgtc cgtcacccat
     8821 aacaccgaca gagcggatcc ctggaagaac agtgaaatat tgccagatat cacggtcaag
     8881 tccagcagct gcgatttctt cacgcaagat agcatcagat tcgcgtacga tttctagctt
     8941 gtcttcagtg atttcgccaa gaacacgaat accaagacca ggaccaggga atggctgacg
     9001 ccaaacgatt gaatcaggca tgcctaactg agtccctaat tcacggactt cgtctttgaa
     9061 taatgtgttt aatggttcga tcaattcaaa ttgcatatct tctggcagtc cacctacatt
     9121 atggtgagat ttgatcgtct gtgctgtttc tgtaccagat tcgataacgt cagtatacaa
     9181 cgttccttgt gctaggaaag agacgccttc ttcaccagca agttttgttg cttcgtcatc
     9241 aaaaacatat acgaattcat tcccaatgat tttacgtttt tgttctgggt cagatacacc
     9301 tttcaacttg tctaagaagc gatctcttgc atcgactttg atgatgttca agccgaattt
     9361 tccgcctaga ctttccatta cttgttccgc ttcgcctttg cgtagcagac cgtggtcaac
     9421 aaagatagat gtcaattggt caccgatcgc tttttgtaaa agaaccccaa cgacactaga
     9481 atcaacgcca cctgaaagac caagcaagac ttttttgtca ccaacttgtt cacggatttt
     9541 ttctacttgc atatcgataa agttatccat tgtccagtta ccttcacagt gacaaacatc
     9601 aaaggcaaag tggcgcagca attcgattcc gtattcggaa tgacgtactt ctgcatggaa
     9661 ttggatacca taaaaattac gtgttgtgtc ttggatagaa gcaatcggac aatctttgct
     9721 tgttccgact gtttcaaagc cttcaggaac tttagttacc aagtcaccat ggctcatcca
     9781 tactgtttgt tgttttggtg tttctttaaa caagacagct tgatcgcttg ttacttctaa
     9841 caaggctttc ccatattcgc ggtttttagc aggctctacg gaaccgccaa gtttgtacgt
     9901 gatcaattgc ataccatagc aaatccctaa tacaggaata cctaaattga agatctcagg
     9961 atcgatccca aatgcattct catcatatac actgtttggt ccgccagaaa gaatgattcc
    10021 ctttggattc atcgctttta cttcttctgc agtaatgcgg tggctcaata attcggaaaa
    10081 gactccaaga tcacgaatac gtcttgtgat caattggtta tactgactac cgtagtctaa
    10141 tacgataatc ttttcaacgt tcgtcatatc ggcaacgttt gtcaaattta ttacccctat
    10201 ccctttaaat tttatctcaa aatagtcaaa ctattttaaa atgctaatat tttcgcatga
    10261 aaatcgaatc gatgatatga ttttcagttt tatggagaat tatatcggct cttcctcgtg
    10321 taggcaagat atattcctgt aaatttttca aattcacatt tttccataca tcttttgcca
    10381 ttttaaaggc ttcttcacga tctccgatag caaaacgata ataataattc tctggatttt
    10441 ggaaagccgt atccagtaac gcaccaaaac gatccaaata ccatttttcg atcagcacgg
    10501 gatcggcatc gacatagaca gaaaaatcaa aaaagtcgct gacgtagatt tgctgattcg
    10561 caggtaattg aagcgtattg atcccctcaa cgatcaagat atcaggctgc tggatcaatt
    10621 cgtacttgtc tggaatgata tcatacacat catgtgagta aacaggtact tttatctctt
    10681 cctgaccaga ttttacacgg ttcaagaaat cgatcagcat ctccatatca taactttcag
    10741 gaaatccttt acggtccatg attccacgct cttttaatat acgatttgga taaagaaatc
    10801 cgtcagtcgt gatcagctga acgtttcttc gtttgaaggt acgagacaaa atcgtctgca
    10861 gcagtctggc tgttgtactt tttccaacgg ctacacttcc ggcgatcccg ataataaaag
    10921 gcggtacttc tacgtattta tgaagaaata aacctttact caagaacaaa gattcaaatt
    10981 ctttcatata aagataaatc agatgagcaa gaggtacata gatatctcga acatcctgca
    11041 tggaaatgcg gtcattcaaa cttttgatat tatctagttc atcttgtgtc aaaggtgctt
    11101 gcccattacg ataaaattct cgccattctt ctcttgccac acggtagtag ttcattggat
    11161 cattcatatt agcctcctgc gttctcccat caatggaact attttaacac aaaataaacg
    11221 caaaccccac cacttaagag agattgccga tctttttgta taatctttat aatagaaaga
    11281 aacggagtct tattcttgac ttgcgcacgg atacataaaa ggatagactg ttgtcatgaa
    11341 agaaatgaga aggatggaag acatgacaaa tcattactat tctgaacgcc cagatacggc
    11401 gcacgatttt gaacaatggg cttttgagtt aaaaggcaga acctttcaat ttgtaacaga
    11461 ttctggtgta ttttcacgtg aaacagtgga ttatggttca cgtgtcctga ttgatgcttt
    11521 cgattgggaa gagttacctg atgaaggaaa gatcttagat gtgggatgcg ggtatggacc
    11581 gattgggtta gctttggcat ttgcgacaca gcggtttgtt gaaatggtcg acattaatga
    11641 acgagcggta agtctagcgc aaggaaacgc aaagcgtaac caaatcgaac atgttgatat
    11701 ccatcaatcg aatatctatg aagcagttca tgaagaaacc tatgcagcta tcgtaagcaa
    11761 tccgccgatc cgtgctggaa aaaaagttgt acatgaaatt ttgacaggcg catatcctcg
    11821 gttgaagaag ggcggtacac tgacaattgt gatccagaag aaacaaggag cgccgagtgc
    11881 gcaaaagaaa atggaagaaa catttggtaa tgcagaaatc gtcacaaaag ataaaggata
    11941 ttatatcatc aaaagtgtga aagaataact agctgaaaaa tagtggagcg acgaaagagt
    12001 ggaactgggc gtgcatgtac gggtacgtat cagttctttt tttgttgaga agtttttaaa
    12061 aacaaagaac tattttttct tcaaaaagaa atagtaggaa cagagtcgtt gttttttacg
    12121 ataaaataaa taagatacag aacttgtata gaaggctgcc aagtcggtag ataaaggagg
    12181 tttttatcaa aaaacatact ttcctttttt ctctattctt ttcttggggg ttttggtaca
    12241 ctgataggga atttagatag aagggagcca ataggatgaa gctgaccatc agagatatcg
    12301 cagaaatggc aggcgtatca gtaacaactg tctcacagat tttgaataac aaaggaagca
    12361 gatttagtga taagacaaga aaaaaagtgt tgtcgatcgt gaatgaacat cattataagc
    12421 ccgattattt cgcttcaaat gtgatcaatc gacattcaaa gaccatcgga atgatcgtcc
    12481 ctgatgtcac tgatttcttc ttctccaaac tgatcgaagg tgtagagagc tatttgaatc
    12541 ctttgggcta tgtgatcatg ttatgcaatt ctcgtcatag ccaagaaaat gagatcaagt
    12601 atttacaaga gctttcccac cgctcagtgg acggaatctt gctggccaca ccaaatattt
    12661 tacctgagaa atatgcatta gacagcggtt tttatcaaaa aatgccaatc attttaatcg
    12721 atcgcggatt gaatcgtcgg gataacggac ggcttatcgt aaaggaatat gagggcgcct
    12781 atcaagctgt ttcgctgttt attgaaaatg gccatacgaa gatcggcatg ctgaaggaaa
    12841 ccactggata ctatcaatta gaagaacgtt tcaacggata tcgtcatgcg ctgaaagatc
    12901 atggcctgcc ttttaatggg aaatttgttg aacatggaga gttgactgtc caaggtggat
    12961 atactgcatc aaaaaaatta ctcaaacata aagatatcac ggctattttt tgcgggaatg
    13021 atgccatggc gatcggatgc tatcaggcga tcgatgaatt gggcaaaaaa atcccagaag
    13081 atatctcagt cataggattc gatggattaa agctgtcaga atacatgatt ccgcaattga
    13141 cgacagtgca gcagccaagt tttgatattg gattttatgc tgcacgtttc ctgatcgata
    13201 cgatcgaatt tcctcaaaga aaagttccga acaaagtgtt cgaaacaaaa ttaattattc
    13261 gtgagagcgt aaaagtattg acaaagggct aactcggcgg tatattgctt atgtaagcat
    13321 ttacaaaggc tagttagtgc tttttttatt tttagtaaac cagtttacta aagtggttta
    13381 cctttcctaa aaataatgta taataccgaa tgaagacaag gaggatcagg caatgcgaat
    13441 ggtggatctg attgaaaaaa agagggatgg aaacgagctg tcgaaagagg aaatcgagta
    13501 catcgtcacg aactatacga atggaaagat tccagattat caagtcagtg cattattgat
    13561 ggcaattttt tatcaagaca tgacaaatga agaaatcacg aacttaacat tggcgattgc
    13621 taattctggt gatgtcatcg atttgagttc tcttgaagga ataaaggtag ataagcattc
    13681 gacaggtggc gttggtgaca cgaccacatt gattttagcc cccctagtag ctagtgtcgg
    13741 agtgacagtg gctaaaatgt ctggacgcgg tctaggttac acaggtggta cactagacaa
    13801 gctagaagca attcccggat ttcagatcga gctttcggat gaagaatttg tacgcattgt
    13861 taatgaaagt aaagtagcgg taattggtca atcaggaaac ttggcaccgg cggacaaaaa
    13921 attgtatgcc ttgcgtgacg tcacagcgac cgttgattct cttccgctga ttgccagttc
    13981 gattatgagt aaaaaaatcg cggcaggagc agatgcaatc gttttggatg tgaccactgg
    14041 agacggtgct ttcatgaaga acatcgaaga tgcaagacgt ctggcaaaga ccatgacgag
    14101 cattgggaaa ctggctaacc gagaaacagt ggcggttatc tcagatatgt cagaacctct
    14161 tggcgaagct atcggaaata gtctagaagt tgtcgaggca atcgaaacat tacaaggaaa
    14221 tggtccagaa gatctagtag aaatgtgcta cgccttagga agccaaatgg ttgttcttgc
    14281 tggcaaagct aagacaaccg atgaagcaag aacgttgctt caagaagctc tggaatcagg
    14341 taaagcgtta gcaaaattca aagaaatgat ccaaaatcaa gggggagacc cagcgatcgt
    14401 ggaacatcca gaacgaattt tgactgcacg ctacacaatg gagcttcctg ctaaacaaag
    14461 cggagttgtg tcaaaaatcg tggcaaatga attaggaatt gctgcaatga tgctaggtgc
    14521 tgggcgtaaa accaaagaag aagatattga tcacgctgtt ggattgaaac tgcataaaaa
    14581 aattggtgac actgtgacaa aaggtgagtc actattgacg atctatagta acgacgaaga
    14641 aatcacttct gtcattgaat tattatataa aaatattgaa attggtgaat cggcaatgaa
    14701 gccaacgttg attcatgaca tcatcacaga ataaaggaaa aaagcggact ttaccgtttt
    14761 aaagaaaagg ggaaaagaaa tggaactaaa ccgaatgatc gaccatacta ttttgaaagc
    14821 agacgcatct aaagaagctg tgatgcaaat tatcgaagaa gcaaaaaaat accattttta
    14881 ttctgtctgc atcaatccag catgggttgc gctagcgaaa gaacaattac aaggtgagcc
    14941 agtagccgta tgtacagtaa tcgggtttcc tctaggagca aacacacccg agacaaaagc
    15001 atttgaaaca acagatgcca tcaataacgg tgcagatgaa gtcgatatgg tcatcaatat
    15061 cggtgctttg aaatcaaagg atgatcaaca agtccaaaaa gatatcgaag cagttgttga
    15121 agcagcaaaa gatcgtgcgc ttgtcaaagt tattattgaa acatcgttat tagatagaga
    15181 agaaatcatc cgagcttgcg aaatagcaaa agctgcaggc gctgattttg tcaaaacatc
    15241 aactggtttc tcaacaggcg gagcaaaggt agaggatgtc cgtttgatgc gtgaaactgt
    15301 aggaccagaa atgggtgtca aagcttccgg cggtatccat aacgaagaag aagcaatggc
    15361 aatgatcgaa gctggtgcta caagaatcgg aacaagcgca ggagtagcaa tcgtatctgg
    15421 ttcaactggt gaaggctatt aattttttca aaggagaagc tcatggcaga actcaaacaa
    15481 gaatggatca acacagctat tgaagcgtta gataaagcct atgttcctta ttcccatttt
    15541 ccggtaggcg cttgtctcgt aacggaatca ggcaagattt atcaaggcat caacattgag
    15601 aatgcatcat ttggattaac gaattgtgcc gagcgcacag cttttttcaa agcagtatct
    15661 gaaggagaaa aaacatttac gcatctagtc gtagcggggc acacccccga tcctatctct
    15721 ccttgtggtg catgtcgaca agtaatggca gaattctgtt cccctgatat gccggttacg
    15781 ctagtaggag acaatggtgt gacgaaggca acgaccgttc gtgcactgct gccttatgca
    15841 tttactgaaa aagatctata actcatgttc attcagtcag ctgactgttg aaaaataaaa
    15901 caattatttt aggaggcttt acagaaatga aaaaagcaaa attatttggt ttaggtgctg
    15961 tcgcactagc agcagggtta tttttaggtg catgcggcaa caacggctct actgattcat
    16021 ccggtggcaa aagctcatca gacacaacaa ctgcagcatt gatcacagac acaggtggtg
    16081 tagacgaccg ttcattcaac cagtcagctt gggaaggact agaaaaatgg gggaaagatc
    16141 aaggactttc acgaggaaat gatggcttcc aatatttcca atcttcaaat gaatcagatt
    16201 acatcccaaa tattgaccaa gcattgaatg ctggattcaa aacgattttt ggtattggtt
    16261 ataaactaaa accagcgatc caagaacaag caacaaacaa tacaggtacg aactttgtta
    16321 ttatcgatga tgtcatcgat ggattagata acgttgtatc tgcaactttc aaagacaacg
    16381 aagcttctta tcttgcaggt gttgcagcag cttatacgac tgaaacaaac gtagtcggct
    16441 ttatcggcgg tgtaaaaggt gaagtaatcg atcgttttga tgctggattc aaagccggtg
    16501 ttgatgcagg tgcgaaagaa ttaggaaaag aaatcaaagt attgaatcaa tatgctggtg
    16561 acttctctgc tccagataaa ggacgttcaa ttgctcaagg tatgtatgca caaaacgcag
    16621 atatcatctt ccatgcttca ggcggtacag gtaacggtgt attccaagaa gcaaaatcat
    16681 tgaacgaatc aggcgacaaa aaagtttggg ttatcggtgt tgaccgcgac caatcagacg
    16741 aaggcgaata cacattaaac ggcgaaaaga aaaacttcac attgacatct acactgaaag
    16801 cagttggaac agtagtagaa gatttggcac aaaaatcagc ggacggtaaa ttccctggcg
    16861 gcgaacacac tgtttatgga ttgaaagaag acggtgttgg tctaacagaa ggacaacttt
    16921 ctgacgaagc gaaaaaagca gttgatgaag caaaagaaaa aatcatttca ggtgacgtaa
    16981 aagttccaga aactcctgaa gaaaactaaa accatttcta tagccttgtt agacacatgt
    17041 ttgacaaggc tatggtttga ttagaacaag aattgttcat ttatccaagc tgccttgtga
    17101 cagtttggat aagggaacaa ctccctgaag taatgtttgt ttcaaaagaa gaacgctcac
    17161 taagcgtgaa tcttagtcaa tcgttgccct agatagaaat cgttaggatg tgaaaaaaag
    17221 gtggttcaag aaaatattgt catagaaatg cagaatatta ccaaagaatt tggaacattc
    17281 aaggcaaatg acaacatcaa tttacaagta aaggcaggag aaattcatgc gttgcttgga
    17341 gaaaacggtg ctggcaaatc tacattgatg aacgtgcttt ccggattatt agagccgaca
    17401 tcagggaaaa ttttgatgcg tgggaaagaa gtaaagatca caagtccgac caaagccaac
    17461 caattaggga ttgggatggt ccatcagcac tttatgcttg ttgatgcctt tactgtaaca
    17521 gaaaacatcg tgttgggaag cgagcctagt cgcgcaggga tgcttgacca taaaaaagca
    17581 cgaaaagaga tccaaaaagt ttctgaacag tatggattgt cagtcaaccc agatgcttac
    17641 gttcgtgata tttcagttgg aatggaacaa cgggtagaaa ttttaaaaac actttaccga
    17701 ggagcagatg tactgatttt tgatgagccg acagctgtat tgaccc
//
LOCUS       DACEEZ010000056        17559 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20313_2017
            SAMN12639966-rid9879223.denovo.056, whole genome shotgun sequence.
ACCESSION   DACEEZ010000056 DACEEZ010000000
VERSION     DACEEZ010000056.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639966
            Sequence Read Archive: SRR10023158
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 17559)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17559)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741683.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741683.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:44:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,016
            CDSs (total)                      :: 2,962
            Genes (coding)                    :: 2,889
            CDSs (with protein)               :: 2,889
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 36 of 73
            Pseudo Genes (incomplete)         :: 29 of 73
            Pseudo Genes (internal stop)      :: 18 of 73
            Pseudo Genes (multiple problems)  :: 8 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17559
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639966-rid9879223.denovo.056"
                     /isolate="E20313_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            <1..237
                     /locus_tag="IWO89_001901"
     CDS             <1..237
                     /locus_tag="IWO89_001901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autolysin"
                     /protein_id="HAQ0456632.1"
                     /translation="IQMMDIEAERDAILAALMALSLISRQILYYSFCVPDSFSNYRIS
                     REVGYSERSIQRMKSEALIEFAEAYKHGRIIAYK"
     gene            583..957
                     /locus_tag="IWO89_001902"
     CDS             583..957
                     /locus_tag="IWO89_001902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0456633.1"
                     /translation="MEDKMYLRQKVFTKKDIENQINGFIQKLENANSYLINKELNKAY
                     DQWKEVYDELKLIQNETKLVRTEKKNENSFFFDGYAILMLETVAKQNIKAPKKELSDN
                     IDNALAELRYYVMQIKDVRITR"
     gene            1187..1531
                     /locus_tag="IWO89_001903"
     CDS             1187..1531
                     /locus_tag="IWO89_001903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HAQ0456634.1"
                     /translation="MNEEFYRWLLQLIREDRLVKFYQSPKWRRLREKAMKRDHYECQE
                     CRRLGKYHRVENVHHIKEVKDRPDLALDLDNLICLCVEHHNEVHGRYLTALDKQEKKI
                     ESFANFDASERW"
     gene            1536..1817
                     /locus_tag="IWO89_001904"
     CDS             1536..1817
                     /locus_tag="IWO89_001904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0456635.1"
                     /translation="MIINDNGREYDTEKIEEYSSYTQGLIKRLIYVRYVGIRDLLSDN
                     CCSKYKVNQVREALNKDNNVERIKNVFGYSIEEINYYIDFAEAFIPMVR"
     gene            1920..2234
                     /locus_tag="IWO89_001905"
     CDS             1920..2234
                     /locus_tag="IWO89_001905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase"
                     /protein_id="HAQ0456636.1"
                     /translation="MFKNELSQNRYREKLRRSLISQLESQKTNIEPFLDNVDRYISLW
                     ETAISLEEDISENGIRLENGKKNESVALLVSVNKQMGLMLDKLAITPELVGEANESIP
                     EL"
     gene            2212..3906
                     /locus_tag="IWO89_001906"
     CDS             2212..3906
                     /locus_tag="IWO89_001906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase large subunit"
                     /protein_id="HAQ0456637.1"
                     /translation="MNQFLSYKHIENWFKAIEEGTIKVCKEQLLLKNYLEERVFTRED
                     IYFDKQMVEDSINIPAQYFPFELIPWEKFLQCFIYGVRWKKDKTLVFNRYLSLMGRGN
                     GKTGFASWNNFFLLTAKHGIKNYDIDIYANNESQAKTSFDDVFKVIKDHPDLDKKVFK
                     ATKEVIQNIATNSKLRYNTANARTKDGKRPGANRFDEIHENEDYSMINVATSGGGKIR
                     DYREFYDTTNGHVRGGPLDDIIEESKMILSGELGIDKDGAEFSSLFPFICRLDNDNEV
                     DDPDMWEKACPTINYNANLKRKMFQEYSQMQRNAGLRLTFMTKRMNRPMEDTRFAVAS
                     YDDVLHTKEKEFPEKMDEVIGTVDFADRRDFASVGLLGKYDKDVYFTQHTFIHESALR
                     LQNIKREVIDISIDQGKSQIVHGKNIEADYIVGWFLEMSNKYYIKKIAMDMYRAKILK
                     PALEEAGFTVEIVRSGSVTHGMLKDLVDDLFINQRLFFGDDAIMRWYCMNVYEEHISN
                     GNIRYEKIEPETRKTDGFFSFLHGLNFLDDIYDSAPVTVTNSSVENTGTGFTPLVF"
     gene            3926..5104
                     /locus_tag="IWO89_001907"
     CDS             3926..5104
                     /locus_tag="IWO89_001907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HAQ0456638.1"
                     /translation="MGIFQKAVGYFTKKATVPLEEYFCKLQVDFVYRKFAIETCIDLI
                     ANAMSKAEFKSYEDGKNKKNDLYYRLNVAPNKKNNATEFRKKLIRRLIFYNEVLIVSP
                     SNNSSEIFIADSWDVTEYALKDDVFSQVQINNIVLDREFLESDVIYIKYADQQIRQLV
                     DAYYQAYGKLISSAMNVYKRSNARRYVLKGNLFRPQDNTTQDQINKMMTSQFKAFMEA
                     DNAGAVFQLQNEYTLEDFSGNFQSNSRDIKNLIDDIFEMTAAAFHVPKNLLKGDMSGL
                     SDQVDAFLMFEIIPIAELIQDAFNASLYEVEEYLSGNFVRVDTTMIKITSFKDLVDAI
                     DVGIRNGVFTINEGRERVGNDRSDKAMADEIFITKNNQQVSKGGEANDDNENISSSKE
                     "
     gene            5067..5753
                     /locus_tag="IWO89_001908"
     CDS             5067..5753
                     /locus_tag="IWO89_001908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Clp protease ClpP"
                     /protein_id="HAQ0456639.1"
                     /translation="MTTMKTFLAVKNEGAVPQIFIQGFIGSSWFFEGNTDKGIKNILD
                     SLGDQEEIEVVINSNGGDVFQGIAIGNLLKSNKAKVNVVINGLAASAASIIAMAGDTI
                     KIYNNAQLMIHRASTYGEGNVDDFRTIADQLESIDKSVKASYKTRFNGTDEALQELLE
                     KESFMDAETALSYGLVDEIIDAENSSGTEAKKEQSVEEILNDVKEKRAEKIAAFTAAL
                     NKTFGQGDVK"
     gene            5753..6913
                     /locus_tag="IWO89_001909"
     CDS             5753..6913
                     /locus_tag="IWO89_001909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein"
                     /protein_id="HAQ0456640.1"
                     /translation="MTVKNLKGVTAASDQLMKAFKDGNEESFSAAMVSLSKEIQDKIL
                     EEATAKNQDQLVLMNRGQRVLTTQETKFYNEVVKNEGFAGVEELVPATVFERVFEDLE
                     QSHPLLQKITFVNTTGVTEWIVSRGVNPAWWGKLCEAVKKVLDNGFDVINMKQFKLSG
                     YIPVCKAMLDLGPVWLDRYVRTVLVESLRIALEQAIVDGTGKDMPVGMMRDMSKQTSG
                     EYAEKKAEPITALDAVTMGRLMARLSKFNIEGVNDPIYRNVNPSDVVLIVNPTDYWSK
                     VFPAKTVLTANGEYVQVLPVPVSDLQSTAVPEGKAVIGVASDYFMGVGSTLKIEASDE
                     YHFVEDERIYLAKQYANGQPKRNDSFIVLDISALGTTTTTTKPTTTTTTTQA"
     gene            6923..7798
                     /locus_tag="IWO89_001910"
     CDS             6923..7798
                     /locus_tag="IWO89_001910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0456641.1"
                     /translation="MKYILCQPAINRFKWELEVCLTNLKKLGIKDIVLLFSRHDDQIP
                     IFFEKEYGVEVHVYDDLRDDKEYIPSIKPYLWWKYLEEDHSREDDRYFYIDSDVIFNK
                     RINLRKLPSKDDVWYCSDCCSYLSLDYIRSCENGENILKDMANIVNVTVESLETINTN
                     SGGAQWVINRPKANYWKKVYLDSNRLYRYLRGQKTNIQIWTAEMWARLWNMMYFNIGP
                     KVHEELDFCFATDPIEKVKEVKILHNAGVTTNDEDLFFKGRYVTSTPFDEDLSFVNKK
                     KCSYAYVKAIKAVVR"
     gene            7795..8106
                     /locus_tag="IWO89_001911"
     CDS             7795..8106
                     /locus_tag="IWO89_001911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0456642.1"
                     /translation="MTPEQVTEELLIAVKDNIYVTWNEEDESIKKMIAKNAVYLQSKV
                     STTLSFSPESLEYGLLIERCRYDWNRALDEFEQNFASELLGFIQHYALQEYIAGDVNG
                     E"
     gene            8096..8449
                     /locus_tag="IWO89_001912"
     CDS             8096..8449
                     /locus_tag="IWO89_001912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage head closure protein"
                     /protein_id="HAQ0456643.1"
                     /translation="MANNRRLEETFNDGWLKILTQTTKRNELGKKIGVEDTEITSLKF
                     RNLSMRDSDITAMDAMGSKLTKKVKTPFHPIAKKFNKDQYFIVIDSMRYNVIYADYDN
                     FYIYFYLESVGEYGD"
     gene            8439..8840
                     /locus_tag="IWO89_001913"
     CDS             8439..8840
                     /locus_tag="IWO89_001913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0456644.1"
                     /translation="MVIDNSKEKERLNKQISAIKTSLEEHFGLKLFQDSVGEDELPDD
                     FNYFILETGEIEMITEPKYSVGQNLYLTFYSENREDLTGDSLDIISLIQNRSIRFQRM
                     DPNHLKLENQDRYIDQLVFTFRRLLKSDCHG"
     gene            8833..9237
                     /locus_tag="IWO89_001914"
     CDS             8833..9237
                     /locus_tag="IWO89_001914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0456645.1"
                     /translation="MAKNSWELKINGHDELLVRMERYSSESERLINEALKSKGSAIAV
                     DRITEKIPVSEADLRRGHQHAKNSRPLKTQYINLGFIIRPTRKFEYLKYPDLGIGTSK
                     RNQPDEFMRRGLGLALDPITELLIRQFDKLNK"
     gene            9249..9857
                     /locus_tag="IWO89_001915"
     CDS             9249..9857
                     /locus_tag="IWO89_001915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HAQ0456646.1"
                     /translation="MAKTKTVVTTFDNVSIKRIAFNFKNAGNAIATNCNGQLDGETEM
                     QTVVKKCGATEVKSKSKPINMTVTITAHVPMEVYRRFNGLKQDGRIKPGIYSYGPDSV
                     GEDFSLAAEIVDDFEENRKLVGMLACTSNTGLTFSIENGADEVAALELETKVMQDEFG
                     KFYHEAIVAELEEDLTDQWMTNLSADVIKKTSVVTTTATQSQ"
     gene            9877..10239
                     /locus_tag="IWO89_001916"
     CDS             9877..10239
                     /locus_tag="IWO89_001916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0456647.1"
                     /translation="MNEDYSKIELNDGTILNLEPKLNIKKLLMINRDFNTDEFAKMTV
                     GKGSMDISVIQGAKAVYIAYRQANMTDYISFDEFIDKWDFDMATASYIYQLMMFKQAR
                     DAYQKEFEKANKEKKLQK"
     gene            10242..10424
                     /locus_tag="IWO89_001917"
     CDS             10242..10424
                     /locus_tag="IWO89_001917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0456648.1"
                     /translation="MPKLLVETWVDVYSMLTDVFSMPSDLVLSDICLDDILQMAYNKS
                     AYEGWKNYAINQSQKN"
     gene            10441..13860
                     /locus_tag="IWO89_001918"
     CDS             10441..13860
                     /locus_tag="IWO89_001918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HAQ0456649.1"
                     /translation="MAKKRTEAEVTFIANDDGLKSTLKEISAELTKNRAELKLEQAQL
                     QQTGSESDKLGSKLSSLEKQYELQSQKVEVTSQRLANAKKYYGENSTEVQKLERELIN
                     QQTAQQRLSNEIDKTSNTLAQAKGEIQTYESTMQQLDSEQKNVQASASLIESEYKKWQ
                     VTAGQSASESEKLAKAQEYVSQQSENAEKTIDILRRQLEATQSEFGATSTEAMQMEAK
                     LNDAEREFEELGQAAKNVDTTNLDDIGSKIDMNNLMEASDVLSDIGDKLTELGKQAVD
                     SANSVGSSQSKIQANFGLTKQEAEELTNVARDIYYKGFGESLDQSTDALILVKRNLGD
                     LNNQDLQNITEQAMVLENTMGADMDETLRGVNGLMVNFGLSAQDAMDLMVSGTQNGLD
                     KTHELGDNMAEYSQLWSQMGYSADETFGMLQNGLDAGAYNLDKVNDLVKEMGISLTDG
                     RFEQNMDMFSESTRKAFEEWKNGGGTQKDVINSMIQDFSNMDGQYDQLNKASTIWSAL
                     GEDNAMKVVQSLTDVNHTFDDVSGSAQKMNEDSTTPLQELNGKIAELKDSLAPIGNTI
                     IDALEPVIDFLGKMADAFNNLPQPVQDYAVAIGGLTAAFTLLMPIIVGFMALGGPTTL
                     IIGAVITAIAGVIAIIKNWGAITDWFKGIWSKFTDWLGGTWESIKEGASSVWDGVKET
                     WSGFVDWVQEIWQGVSDWFGELWSGLVEGASNIWQGVQETWQAFVDWVSNIWNGVKEV
                     WSIIWADIVGIVQIPWTLITSLIQAGINIIVGIFDVAGQLLGAAWQAVWTPISDFLKN
                     TWDTMTQWISIAWNGIVTTFHTIFDPVVAWWNGIWTSISTTASNIWNSISATASSIWN
                     SIKNTITSLVQAAATVIQNIWSTVSSWLGGIWNSISSTASNIWSSVTSSISNAINAAK
                     SAIQSVWNSISSWISGIWNGIKNTALNLWNGITSTISSKVNDGKNAISSGWSNLTGIV
                     SDIFNNVKSTIANIWEGIKKTVSAPIDWIRDKISSVFDNLNISIPHIPLPHFKLSGEF
                     NPLKGKIPTLGVDWYAKGSVFNSPNIIGVGESGPEAVLPLKRSVLQEIGDRILSSTSV
                     SSRTQAVQPVNNYEFNFTIDGNADEVTMKQTTQQIIDSITKVQNDNASAWR"
     gene            13911..14648
                     /locus_tag="IWO89_001919"
     CDS             13911..14648
                     /locus_tag="IWO89_001919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HAQ0456650.1"
                     /translation="MTDCIHSIIDGFPDYLHKLALAERPTIPSPKRQRVETSVLGRLG
                     GLVQDYSFEDMSFTLHYNYLEDVEDHQAFKQSFYIMRHWLNYAKKLEFSDDPNVYYVI
                     QTIDIGDAENDIVEWGEFDVNITAKPFARVQEDVPITVDKPQSFNLLNNSLEESFPKI
                     IITPSATSCQFILNDYVFSFEGLVAGTDVVIDSDLMLCYEEQSDGDILDRSNKMKTMQ
                     YPTLQVDINHFNCTGLSKIQIYRNGLR"
     gene            14658..16949
                     /locus_tag="IWO89_001920"
     CDS             14658..16949
                     /locus_tag="IWO89_001920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0456651.1"
                     /translation="MIDNLITIYDKNDANNLAEHLYDTQGLGALSDWLTATVSNKLNG
                     AEIFQGTYPISGTNADLIVEGRIIQCYVDENRAKQRLRIYYAKTSVIGNTIEVKAEPI
                     FNDIRKSVLNKYDSGTEKITATQAWQNAKALAKPAIPSQFSFSSLVDTLANVKIEKAN
                     FLEFFGGKEGSILDRFHGEFLKDNNTLHHEKRLGTDHKIKAIYTKNLTGLDLEIDAQS
                     VLVGVYPFISSSSEGEDEITLPEEVIFTDYVDDYPAGYVSFVDFKDKATDVATLREAA
                     KDWLKTNIDKQKPQVSGSIELVPLRHQRGYEKFVDLEKVSMGDGVDVYHPQLKVNMSA
                     RIVEYTFNVLTNSYDKLVVGNVKTNFLENTENNVSNLINDAIDQLKNGGEISDLINDI
                     VDHQTDMITGQNGGYVLLDPKEAPSRILIMDTPDKNTARNVLQINNAGIGFSKTGING
                     TYETAWTLDGGFNASFITAGEIVGITIRGTTLISDGTDYRTSIANGKMTWYSKKVNKD
                     IMELEARDYVSADAGIVSYTMKTGGGFMIRNPQGNLVFSTWDNGNNRPFLSFGAPNFR
                     YSNASYVTSGDGSSLSINGSAGNSWEFKVAGRTMKFTSDGMLTLPGCFFGSWEDGKLA
                     RFEQSTVQVYKDFTVRGTKNSTVPTEHYGQRLLNAYETPEYYFADYGEAVTGDDGKVR
                     VDIDPMFAETVNLSRYMTHVTPTELVLCAVTHEDIDHFIIETSKPNVLVRWNLVAHRL
                     GYEDIRLKEDTAYDSTVLDQKRF"
     gene            16973..>17559
                     /locus_tag="IWO89_001921"
     CDS             16973..>17559
                     /locus_tag="IWO89_001921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0456652.1"
                     /translation="MASSLYNLALDFSKELNYTKAIMARQGDKGITVTVKPFLNGLQM
                     DTSGGTFTLKGTTPSNRYVDSVATSVTSEEVTFSLDGTFMSEAGYYKHCYVEYRKDNQ
                     ILTTQDIIFFSLGVSDISQGQADEYVSQLEELIQKYNETFDAFMAEIEGRVDSLNKQI
                     TDLTGQAKTLQDKLDALKEEISKLGNLQVMYSNSI"
BASE COUNT         6256 a         2552 c         3699 g         5052 t
ORIGIN      
        1 attcagatga tggatataga agcggagaga gatgcgattc tagcggcttt gatggctctt
       61 agtctaatta gtcgccagat actctactac agcttctgtg tgccagatag cttctcaaac
      121 tacaggatta gccgtgaagt gggttattca gaaagaagta tacaacggat gaagtcggaa
      181 gctctaatag agtttgcaga agcatataaa cacggaagaa taattgctta taaataattt
      241 ggcggttttt tggcggaatg atggcggttt ttagctattt accagtgata ttatggtagt
      301 gtcgaaagat tagtgatagg tctaagacaa aataaaatgt aagggaggaa atctccctca
      361 tcgtttaatt aagcttcgat agacagcagc aaataaacta aaggatgtgg gggtcagctc
      421 ctacagttag ttcatgtgtt gctgtctatt aattgcaact ctttcggttt tattgagtat
      481 ttactcaact taaaaaaact gttgtttgta gatgtaagct gttcctattt gtacatagta
      541 aaaaatgata taataaaaat attacgaaag gggtgagagt gcatggaaga caaaatgtat
      601 ttaagacaaa aagttttcac aaaaaaagat attgaaaatc aaattaatgg atttatacag
      661 aaattagaaa atgcaaattc ttatttgata aataaggaac taaataaagc ttatgaccag
      721 tggaaagaag tatatgatga gttgaaattg attcaaaatg aaacaaaatt agtacgaacg
      781 gagaaaaaga atgaaaatag tttctttttt gatggatatg ctatattgat gttggaaact
      841 gtagcaaaac aaaatataaa agctcccaaa aaagaactat ctgacaatat tgataacgca
      901 ttagccgagt taaggtatta cgttatgcaa ataaaagatg ttaggatcac tcgttgagtg
      961 gtctttttat tttgcacaaa ggaggtaaca acaatgtata gaccacaata cttagaacag
     1021 aagtatgaag taatcactgt gcaaaatggt aacggagaga tagtacgaaa gtatagaaga
     1081 ccaataaaga gcgatacata taaacgaaag gaaagcaatg aagttattcc attgtatggc
     1141 aaaagaatag ctaagcatta aataagattg tgaaaggaga cgggacatga acgaggaatt
     1201 ctatagatgg ctattacagt tgataagaga agatcgtttg gttaagttct atcagtctcc
     1261 taaatggcgc aggcttagag agaaagcgat gaaacgagat cactatgaat gccaagagtg
     1321 tagaagacta ggtaagtatc atagagtaga gaacgttcat catataaagg aagtcaagga
     1381 tagacctgac ttagctttag atttagataa tcttatttgt ttatgtgttg aacatcataa
     1441 tgaagttcat ggcagatatc ttacagcatt ggataaacaa gagaagaaga tagaaagctt
     1501 tgctaacttc gatgcaagtg aaaggtggta agtgcatgat catcaatgat aatggcagag
     1561 agtatgatac agaaaagatt gaagagtatt catcttatac tcagggatta attaaacgtt
     1621 tgatatacgt tcgctatgta ggtattaggg atctgttatc agataactgt tgtagtaaat
     1681 acaaagtgaa tcaagtaaga gaagcgttga ataaagataa taacgtcgaa agaataaaaa
     1741 atgtttttgg atatagtatt gaagagatta attattacat tgacttcgct gaagctttca
     1801 ttccgatggt gagataaccc ccccttaaaa taaatcgcaa attttttggg ggtgatgaaa
     1861 cggagggggc tgtcaggaaa agagattttt tcgaacttta tcatgaaagg agggctaaaa
     1921 tgtttaaaaa cgaattgtct caaaatcgct acagagaaaa attacgccgc tctttaataa
     1981 gccaattgga aagtcagaaa acaaatattg agccattctt agataatgtt gatcgttata
     2041 tcagtttatg ggaaacggcg atatcactgg aagaagatat atccgagaac ggcattagac
     2101 tggagaatgg taaaaagaat gaatcagtag cgttgcttgt ttctgtcaac aaacaaatgg
     2161 gattgatgtt ggataaactt gccattactc ctgaattggt aggtgaagca aatgaatcaa
     2221 ttcctgagtt ataagcatat tgaaaattgg ttcaaagcta tagaagaagg cactatcaag
     2281 gtatgcaaag agcaattatt gctaaaaaat tatctagaag aaagagtctt tactagagaa
     2341 gatatttact tcgataagca gatggtagag gattcaatca atataccagc acaatacttt
     2401 ccattcgaat taattccgtg ggaaaaattt ctacaatgtt ttatttatgg tgttcgatgg
     2461 aaaaaagata aaacactagt gttcaataga tatctttcat taatgggacg tggtaatggt
     2521 aaaactggtt ttgcttcttg gaacaacttc tttctactaa ccgctaaaca cggtattaaa
     2581 aattatgata ttgatatcta tgccaataat gaaagccaag caaagactag ttttgatgat
     2641 gtatttaaag taattaaaga tcatcctgat ttagataaaa aagtatttaa agctacgaag
     2701 gaagttattc aaaatatcgc tacaaacagc aaacttcgtt ataacacggc aaatgctaga
     2761 acaaaagatg ggaagcgacc aggtgcaaac cgctttgatg aaattcacga aaatgaagat
     2821 tattcaatga taaatgtggc tacttctggt ggtggtaaaa ttcgagatta tagagaattt
     2881 tatgatacaa ctaatggtca tgttcgtggt ggtccgcttg atgacattat agaagaatca
     2941 aaaatgattc tttctggaga acttggaatt gacaaggatg gagcagaatt ttctagtttg
     3001 tttccattta tttgtcgctt ggataacgat aatgaagttg atgatcccga catgtgggaa
     3061 aaagcttgtc caactattaa ttacaatgca aatttaaaac ggaaaatgtt tcaagaatac
     3121 tctcaaatgc aacgtaatgc tggtttaaga cttacgttca tgaccaaacg aatgaacaga
     3181 cctatggaag atacacgatt tgctgttgct tcatatgatg atgttctgca tacgaaagaa
     3241 aaagaatttc ctgaaaaaat ggatgaagtg ataggaacag tcgattttgc tgatagacga
     3301 gattttgcca gcgttgggtt gctaggaaaa tacgataaag atgtttattt tacacaacat
     3361 acttttatcc acgaatcagc ccttcgatta caaaacatca aacgagaggt tatagatatt
     3421 tctatagatc aaggaaaatc acagatcgtt catggaaaaa atatagaagc tgattatatt
     3481 gtaggttggt ttcttgaaat gagtaataaa tattatatta aaaaaatcgc tatggatatg
     3541 taccgtgcaa aaatattgaa gcccgcttta gaagaagcag gttttactgt ggaaattgtt
     3601 cgaagcggat ctgttacaca tggtatgtta aaagatctgg ttgatgacct ttttattaat
     3661 caacgtttat tttttggtga cgatgcgatt atgcgttggt attgcatgaa tgtatatgaa
     3721 gagcatattt ctaatggaaa tatacgctat gaaaaaatag aacctgaaac tagaaaaacg
     3781 gatggctttt tttcattcct tcatggtttg aattttttag atgatattta tgattctgct
     3841 cctgtaacag tcacaaatag ctcagtagaa aatacaggaa ctggatttac tcctctagta
     3901 ttctaacttg aaaggaggtg agaaagtggg gatttttcaa aaggcggtag gatacttcac
     3961 aaaaaaagca acggttcctt tagaagaata cttttgtaaa ttgcaagttg attttgtgta
     4021 tcgaaaattt gcgattgaaa cttgtattga tttgattgca aatgcgatga gtaaagcgga
     4081 attcaagtca tatgaagatg gaaaaaataa aaagaatgat ctttactata ggctgaatgt
     4141 agctcctaat aagaaaaata atgcaacaga atttagaaaa aaactgatca ggagattaat
     4201 attctacaat gaagtattga tcgtttctcc gtctaataat tctagcgaaa tatttattgc
     4261 ggatagttgg gatgtcacag aatatgcatt gaaagatgat gtgttttctc aagtgcaaat
     4321 taacaacata gtccttgata gagaatttct agaaagtgat gttatctata taaaatacgc
     4381 agatcaacaa attaggcaac tagtcgatgc gtattatcaa gcgtatggga aactcatttc
     4441 tagtgccatg aatgtttaca agcgctctaa cgctcgtaga tacgtactga aagggaattt
     4501 attccgaccg caagacaata caacacaaga tcaaatcaat aaaatgatga catcacaatt
     4561 taaggctttt atggaagctg ataatgcagg tgcggtattt caattacaaa atgagtacac
     4621 attagaagat ttcagcggaa acttccaaag caattcaaga gatataaaaa acttaataga
     4681 cgacatcttt gagatgacag cagcagcgtt tcacgttccg aaaaacctac taaagggaga
     4741 catgagtggg ttatcggatc aagtggacgc ttttttaatg ttcgaaatca taccgattgc
     4801 tgaacttatt caggatgcgt ttaacgctag tctctatgaa gtagaagaat acttgtcagg
     4861 gaattttgta cgtgtggata caactatgat caagattact agcttcaaag atttggttga
     4921 cgctattgat gtaggcatta gaaatggagt atttacaatc aacgaaggaa gagaacgcgt
     4981 tggaaatgat cgctctgata aggcgatggc agatgaaata tttataacta aaaacaatca
     5041 acaagtatcg aaaggaggtg aggcgaatga cgacaatgaa aacatttcta gcagtaaaga
     5101 atgaaggcgc agtaccgcaa atttttattc agggatttat tggttctagt tggttctttg
     5161 aagggaatac tgacaaggga atcaaaaata ttttggatag tctaggtgat caagaagaaa
     5221 ttgaagtagt aattaattca aacggtggag acgtatttca agggattgct attgggaact
     5281 tacttaagtc aaataaagca aaagttaacg ttgtgattaa cggcttagcc gctagtgctg
     5341 cttcaattat cgcaatggct ggcgatacta taaaaatcta caacaatgca caattgatga
     5401 ttcaccgcgc ttccacatac ggagaaggaa atgtcgatga cttccgtacg attgctgacc
     5461 aactggaatc aattgataaa tcggtaaagg cttcatataa aacacgattc aatggcacag
     5521 atgaagcatt gcaagaactt cttgaaaaag aatcgtttat ggatgcagaa acagctttga
     5581 gttatggatt ggtcgatgaa attatcgatg cagaaaatag ctcaggtact gaagctaaaa
     5641 aagaacaaag cgttgaagaa attttgaatg acgttaaaga aaaaagagca gaaaaaattg
     5701 ctgcatttac agcagcatta aataaaacat ttggacaagg agatgtaaaa taatgacagt
     5761 taaaaattta aaaggtgtaa cagctgcaag cgaccaattg atgaaagctt ttaaagatgg
     5821 taacgaagaa tcttttagcg cagctatggt aagcttatct aaggaaattc aggataaaat
     5881 tttagaagaa gcaacagcaa aaaatcaaga tcaattagta ttaatgaacc gtggtcagcg
     5941 tgtattaact acacaagaaa caaaattcta taacgaagta gtgaaaaacg aaggttttgc
     6001 aggggtcgaa gaattagtgc cagctactgt atttgaacgt gtatttgaag atttagaaca
     6061 atctcatcca ctattgcaaa aaattacttt tgttaacaca actggtgtaa cagaatggat
     6121 tgtgtcacgt ggagtcaatc cagcatggtg gggtaaactt tgcgaagctg ttaaaaaagt
     6181 tttagataat ggctttgatg taattaacat gaagcagttc aagctatcag gttatattcc
     6241 tgtatgtaag gcaatgcttg atttaggtcc agtatggtta gatcgttatg tccgtactgt
     6301 tttagtagaa tcattgagaa ttgcattaga acaagcaatt gttgatggta ctggtaaaga
     6361 tatgccagtc ggaatgatgc gtgacatgag caaacaaact agcggagaat atgctgaaaa
     6421 aaaagcagaa cctattacag ctttagatgc tgtaactatg ggccgtttga tggcgcgact
     6481 atcaaaattc aatatcgaag gtgtgaatga tccgatttat cgtaatgtga atccttctga
     6541 tgtggtccta attgtgaatc caactgatta ctggtctaaa gtattcccag ctaagactgt
     6601 actaactgct aatggagaat acgtacaagt attgccagta ccagtttcag atttgcagtc
     6661 aacggctgtg ccagaaggaa aagcagttat tggggtagcc tcagattact tcatgggtgt
     6721 aggatctaca ctaaaaattg aagcttcaga tgaataccat tttgttgaag acgaacgcat
     6781 ttatctagct aaacaatatg caaacggtca acctaaacgt aacgatagtt tcattgtatt
     6841 agatattagc gctttgggaa ctactactac aactacaaaa ccaacaacca caacaactac
     6901 aacacaagcg taggtgatca gaatgaagta tattctttgt cagccggcaa tcaatcggtt
     6961 taaatgggag cttgaagttt gtttaactaa tctgaagaaa ctaggaatca aagatatcgt
     7021 attgcttttc agcagacacg atgatcagat tcctattttt tttgagaagg aatatggtgt
     7081 tgaagttcat gtgtacgatg atctgcggga cgacaaagag tatattcctt cgattaaacc
     7141 atatttatgg tggaaatatt tagaagaaga tcattcgcgt gaggacgacc gatatttcta
     7201 tatcgattcg gatgtcattt tcaataaaag aattaatttg cgcaaattgc cttctaaaga
     7261 tgatgtttgg tattgtagcg actgctgtag ttatctaagt cttgattata ttagaagctg
     7321 tgaaaacgga gaaaatattc taaaagatat ggcaaacatt gtaaatgtta cagtagaatc
     7381 tttggaaact ataaacacta attcaggagg cgcacagtgg gttattaacc gtcctaaagc
     7441 gaattattgg aaaaaggttt atctggattc taatcggcta tatcgctacc ttagagggca
     7501 aaaaacaaat atacaaatct ggacagccga gatgtgggca cggctttgga acatgatgta
     7561 tttcaatatt ggtcctaaag ttcacgagga attagacttt tgttttgcta ctgatccaat
     7621 agaaaaagtt aaagaagtaa aaatcttaca caatgctgga gtaacaacaa acgatgaaga
     7681 tttatttttc aaagggagat acgtgacttc tacgcctttt gatgaagatt tatcatttgt
     7741 aaacaagaaa aaatgctctt acgcatatgt taaagcaatt aaggcggtgg ttagatgacg
     7801 cctgaacaag tgactgaaga attgctaata gctgtgaagg ataatattta cgttacctgg
     7861 aacgaagaag atgagtcaat taaaaagatg atagctaaaa atgctgttta tcttcaaagt
     7921 aaagtgagta caacactttc tttttctcct gaaagcttag aatacggatt gctaatcgaa
     7981 agatgtagat acgactggaa tcgtgcttta gatgagtttg aacaaaattt cgctagtgag
     8041 ttattaggtt tcattcaaca ttatgcgcta caagaatata ttgcaggtga tgtgaatggc
     8101 gaataatcgt agactcgaag aaacattcaa cgatggttgg ttaaagattt tgacgcaaac
     8161 taccaaaaga aatgaactag gaaaaaagat tggtgtagaa gatacagaaa tcacttcttt
     8221 aaaatttaga aatctttcca tgagagatag tgatataaca gctatggatg cgatgggatc
     8281 gaaattaact aagaaagtaa agactccatt tcatccaatc gccaagaaat ttaataaaga
     8341 tcaatatttt atcgtaatcg atagtatgcg ttacaacgtt atctatgccg attacgataa
     8401 tttttatatc tatttttatc ttgaaagtgt gggtgaatat ggtgattgat aattctaaag
     8461 aaaaagaacg tttaaataag caaatttctg ctatcaaaac ttccttagaa gagcattttg
     8521 gcctcaaact ctttcaagac tccgtaggcg aggatgagct acctgatgat tttaattact
     8581 tcattctcga aacaggagaa atagaaatga tcactgagcc aaaatatagc gtgggtcaaa
     8641 atctatatct aactttctat tcagaaaata gagaagattt aacaggagac tcactagata
     8701 ttatttcatt gattcaaaat cgttcgattc gttttcagag aatggatccc aatcatttaa
     8761 aactagagaa ccaagatcgc tatatcgatc aattggtatt tacgtttaga cgattattga
     8821 agagtgattg tcatggctaa aaatagttgg gagctaaaaa taaatggaca tgatgaactt
     8881 cttgtgcgga tggaacgcta ttcaagcgag agcgaacgac tgattaacga agcattgaaa
     8941 tcaaaaggtt cggctattgc agtggatagg attacagaaa aaattcctgt ttctgaagca
     9001 gatttaagaa gagggcacca acacgcaaaa aatagtcgtc cacttaagac tcaatacatt
     9061 aatttgggtt tcatcattag acctacaaga aaatttgagt atttaaaata tcctgatttg
     9121 gggataggta cttctaaaag aaatcagccg gacgaattta tgagaagagg attaggtctt
     9181 gcacttgatc caattacaga acttctgatt cgtcaattcg ataaattaaa taaatagggg
     9241 gaacaacaat ggctaaaaca aaaactgtag taacaacgtt cgataacgta agtatcaaac
     9301 gaattgcttt taattttaag aacgcaggaa atgcaatcgc aacgaattgt aatggacaat
     9361 tagatggcga aacagaaatg caaacggtgg ttaaaaaatg tggagcgaca gaagtaaaat
     9421 caaaatctaa accaatcaat atgacggtaa caattactgc acatgtaccg atggaagttt
     9481 atcgacgttt caatgggttg aaacaagatg gacgtattaa accaggcatt tactcttacg
     9541 gtcctgattc cgtaggcgaa gatttctcac ttgctgcaga gatcgtggat gacttcgaag
     9601 aaaatagaaa gttagttggt atgttagcat gcacttcgaa tacaggatta acattctcta
     9661 ttgaaaatgg tgcggatgaa gtagctgcgt tagaactaga aacaaaagtt atgcaagatg
     9721 aatttggtaa attttatcat gaagcaattg ttgcagaact tgaagaagac ttaacagatc
     9781 aatggatgac gaatctatct gctgatgtga ttaaaaaaac ttcagttgtg acaactacgg
     9841 ccactcaatc acagtaaaaa aaacggaggt agcgaaatga acgaagatta ctcaaaaatt
     9901 gaactaaacg atggaacaat tttgaattta gaacctaaac tgaatatcaa gaaattattg
     9961 atgatcaata gagattttaa cacagacgag tttgcaaaaa tgactgtggg aaaaggatcc
    10021 atggatattt ctgttattca aggtgcaaag gctgtgtata ttgcttaccg ccaagcgaac
    10081 atgactgatt atatttcatt cgatgaattt atcgataaat gggattttga tatggctact
    10141 gccagctata tttatcaatt gatgatgttc aaacaagcac gcgatgctta tcaaaaagaa
    10201 tttgaaaaag caaataagga aaaaaagctt caaaagtaaa aatgccaaag ctcttagttg
    10261 aaacgtgggt cgatgtctat tcgatgttga ccgacgtttt ttctatgcct tcagatttgg
    10321 ttttaagcga tatctgttta gatgacattt tgcaaatggc ttacaacaag agtgcttatg
    10381 aaggatggaa aaactatgca ataaaccaat ctcagaaaaa ctaaagaaag gaggtaaaaa
    10441 atggctaaaa agagaacaga agcagaagta actttcatag ctaacgatga cggattgaaa
    10501 tctacgttaa aagaaatcag tgctgaatta actaaaaata gagcagaatt aaaactagaa
    10561 caagctcaat tacaacagac tggttctgaa tcagacaagt taggaagtaa attatcttct
    10621 ttagaaaagc agtatgaatt acaaagccaa aaagttgaag taactagcca acgtttagcc
    10681 aatgccaaaa aatattatgg agaaaattcc accgaagttc agaaacttga gagagaactg
    10741 attaaccaac aaacagcgca acaacgtttg tcaaacgaaa ttgataaaac gagtaataca
    10801 ctagctcaag caaaaggcga aatacagacg tacgagtcta caatgcaaca gttggatagt
    10861 gaacaaaaaa atgttcaagc tagtgcttct ctgattgaat ccgaatacaa aaaatggcaa
    10921 gtaactgctg gtcaatcagc ttctgaatcc gagaaattag cgaaagccca agaatatgtt
    10981 tctcaacaat ctgaaaatgc agagaaaacg atagatatcc tgagacgaca gttagaagct
    11041 acacagtctg agtttggcgc tacatccaca gaagcaatgc agatggaggc gaagcttaat
    11101 gatgctgaac gtgaatttga agagttagga caagctgcta aaaatgtaga tacaactaac
    11161 ttggacgata tcggaagcaa aatagatatg aataatttaa tggaagcttc tgacgtttta
    11221 agcgacattg gcgataagct tacagaatta gggaaacaag cagtggactc tgctaacagt
    11281 gtaggtagtt cccagagtaa aatacaagct aattttggtt tgactaaaca agaggctgaa
    11341 gaattaacga atgtagccag agacatttat tataaaggtt ttggagaatc gttagatcag
    11401 tccacagatg cattgatttt ggtaaagcgt aatttaggcg atttaaataa tcaagattta
    11461 caaaatatta cggaacaagc tatggtccta gaaaacacca tgggcgctga tatggatgaa
    11521 acgttacgtg gtgtaaatgg cttaatggtc aacttcggat tgagcgctca agatgcaatg
    11581 gatttaatgg tttcgggtac tcaaaacggt ttagataaaa cgcacgaatt aggcgacaat
    11641 atggcagaat atagccaatt atggagtcaa atgggatatt cagctgatga aacgttcgga
    11701 atgcttcaaa atggtttaga tgcgggtgct tataaccttg ataaagtcaa tgacttagtt
    11761 aaggaaatgg gaatatcgtt aacagatggt cgatttgagc aaaacatgga tatgtttagt
    11821 gaaagtacta gaaaagcttt tgaagagtgg aaaaatggtg gaggaacaca aaaagacgtt
    11881 attaattcca tgattcaaga ttttagcaat atggatggtc aatacgacca attaaataaa
    11941 gcttctacaa tttggtctgc acttggcgaa gataacgcga tgaaagttgt ccaatctttg
    12001 actgatgtta accatacatt tgatgatgtt agtggatctg cacaaaaaat gaatgaagat
    12061 tctactactc cgttacaaga attaaacgga aaaatagctg aattaaagga ttcattagct
    12121 cctataggca acacaatcat agatgcactc gaaccagtaa ttgattttct aggaaagatg
    12181 gctgatgcgt ttaataatct tccacaacca gtacaggatt atgccgtagc gattggcgga
    12241 ttgactgctg catttacttt attaatgcca ataatagttg gcttcatggc tctaggtggt
    12301 cctactacat taataatagg agcagttatt actgctattg ctggagttat agcaattata
    12361 aaaaactggg gcgcaattac tgactggttt aagggaatat ggagtaaatt cactgattgg
    12421 ttgggtggta cttgggaaag cataaaagaa ggtgcctcat cagtttggga tggagtcaaa
    12481 gaaacctggt ctggatttgt agattgggtt caagaaattt ggcaaggagt ttctgattgg
    12541 tttggagagt tatggagcgg attagttgaa ggagcttcca acatctggca aggagtccaa
    12601 gagacttggc aagcattcgt tgattgggtt tcaaatattt ggaacggagt caaagaagta
    12661 tggtcgatta tttgggcaga cattgtagga attgttcaaa taccatggac attaataacg
    12721 tcattgattc aagctggtat taatattatc gtgggtatct ttgatgtagc tggacagtta
    12781 ttaggcgcag cttggcaagc tgtttggaca cctatttctg atttccttaa aaatacttgg
    12841 gatactatga cacaatggat aagcatcgct tggaatggaa ttgtaactac attccatact
    12901 atatttgatc cagtagtggc atggtggaat ggtatatgga catctattag tactacggct
    12961 tcaaatattt ggaactcaat tagtgcaaca gcttctagta tttggaatag tatcaagaat
    13021 acaatcacta gcttggtaca agcagctgct acagtaattc aaaatatttg gtcaactgta
    13081 tctagttggt taggtggaat ttggaattca atcagctcta cagcatcaaa tatctggagt
    13141 agcgtgacta gtagtataag caatgctata aacgcagcta aaagtgccat tcaaagtgtt
    13201 tggaatagta tatcttcgtg gatcagcgga atttggaacg gtatcaaaaa cactgctttg
    13261 aatctttgga atggaattac aagcactatt agctctaaag taaacgatgg aaaaaatgca
    13321 atttcaagcg gttggtccaa tctaacaggt attgtttccg acatattcaa taatgttaaa
    13381 agcacgatag ctaatatatg ggaaggtatc aaaaagactg ttagcgctcc gattgattgg
    13441 attagagata aaatcagtag tgtctttgat aatttgaata tttctatacc acatattccg
    13501 ttaccacatt ttaaattgag cggagaattc aatccattga aggggaaaat cccaacattg
    13561 ggtgttgatt ggtatgcgaa aggtagtgtg tttaattctc caaatattat cggtgtaggt
    13621 gaatcaggac ctgaagcagt tttacctttg aaaagatctg tgctgcaaga aattggtgat
    13681 cgtatcttga gtagcacctc agtttcatct aggacacaag cggttcaacc tgtgaacaat
    13741 tacgaattca atttcacaat tgatggtaac gcagatgaag tcactatgaa gcaaacaact
    13801 caacaaatca ttgatagcat tacaaaagtt caaaatgata atgcttcggc atggcgttaa
    13861 acaggagagt atttctcctg tttttttagt attaaaaagg atgtgaaaaa atgactgatt
    13921 gtatacattc tataatcgat ggatttcctg attatttgca taaattggct ttagctgaaa
    13981 gaccaaccat accttctcca aaaagacaga gagttgaaac ttctgtttta ggaaggttag
    14041 gtggcttagt gcaagattac tcgtttgaag acatgtcgtt tacattgcac tataactatt
    14101 tagaggatgt ggaagaccat caagcgttca agcaatcgtt ttatatcatg cgtcattggt
    14161 taaactatgc aaagaaatta gaattctctg acgatcccaa cgtctattac gttatccaga
    14221 ctatcgatat tggggatgca gaaaacgata ttgttgaatg gggagagttc gatgtaaata
    14281 ttactgcgaa accattcgca agagttcaag aagatgtacc tataaccgta gataaaccac
    14341 agtcatttaa cttgctgaat aatagtttag aagaaagttt tccaaagatt atcatcactc
    14401 cttcagctac ttcatgccag ttcatcttaa atgattatgt gtttagtttc gaaggcttag
    14461 tagcaggaac tgatgtagtc attgatagtg atttgatgct ttgctacgaa gagcaatcgg
    14521 acggagatat tttagatcgg tccaacaaaa tgaagaccat gcaatatccg acattgcaag
    14581 tggatatcaa tcattttaat tgcactggtt tgagcaaaat acaaatttat cgtaacgggt
    14641 taaggtaggt gaaatagatg atcgataatt taataactat ttacgataaa aatgacgcga
    14701 ataatttagc tgaacattta tatgatacgc aaggtttagg cgctttgtca gactggttaa
    14761 cagctactgt tagcaataaa ctaaacggag ccgagatatt tcagggtact tatccaataa
    14821 gcggaactaa tgcagatttg attgtagaag gacgtattat tcagtgttat gtagatgaaa
    14881 atcgagcaaa acagcgtcta cggatttatt atgcaaaaac ttctgtaata ggcaatacga
    14941 tagaagtaaa agctgaacct attttcaatg atataagaaa atcggtgttg aataaatatg
    15001 acagcggaac agaaaaaatc actgctactc aggcatggca aaacgcaaaa gctttagcga
    15061 aaccagctat cccttcgcag ttttctttct cgtcattagt agatacgctt gctaatgtga
    15121 agatagaaaa agcgaatttt ttagaattct ttggtggaaa agagggatct attctagatc
    15181 gatttcatgg ggaatttctt aaagataata acacattaca tcatgaaaaa aggctaggta
    15241 cggatcataa aatcaaagcg atttatacta aaaatttaac tggtcttgac ttagagatag
    15301 atgctcaaag cgttttagtt ggagtttatc cattcattag cagttcttca gaaggagaag
    15361 acgagatcac tctaccagaa gaagtcattt tcacggatta cgtggatgat tatcctgctg
    15421 gatatgtttc ttttgttgat tttaaagaca aagcgactga tgtagccaca ttaagggaag
    15481 ctgctaaaga ctggttgaaa acaaacatag ataaacaaaa accacaagtg agtggttcga
    15541 ttgaattagt accattgagg catcaaagag gctatgaaaa atttgttgat ctagaaaaag
    15601 tttcgatggg tgacggagta gatgtgtatc atccacagtt aaaagtgaat atgtcagcga
    15661 gaattgtgga atatacgttt aatgttctaa ccaattcata cgataaatta gttgtaggaa
    15721 acgtcaaaac aaacttctta gaaaacacag agaataatgt aagcaatttg attaatgatg
    15781 ccattgatca attgaaaaat ggtggcgaaa tcagtgattt aatcaatgat attgtagatc
    15841 atcaaactga tatgattact ggtcaaaatg gtggttatgt tttattagat cctaaagaag
    15901 cgcctagtcg tattttgatt atggacacac cagataagaa taccgcaagg aatgttttac
    15961 aaatcaacaa tgctggtatt ggtttttcta aaactggcat taatggaaca tatgaaaccg
    16021 catggacatt agatggcgga ttcaatgcct cgtttattac ggctggtgaa atagtaggaa
    16081 ttactattag aggtactaca ttaattagtg atggcactga ttatagaaca agtattgcta
    16141 atggcaaaat gacttggtac tcaaaaaaag ttaacaaaga tattatggag ctagaagcac
    16201 gtgattatgt aagtgctgat gccggtattg tatcatacac catgaaaact ggtggtggtt
    16261 tcatgattag aaatccacag ggtaacttgg tttttagtac gtgggataat ggtaataaca
    16321 gaccgtttct atcttttggt gcgcccaatt tcaggtatag caatgctagt tatgtaactt
    16381 ctggcgacgg tagttcttta agcattaatg gtagtgcggg taactcatgg gaatttaagg
    16441 tagctggtag gactatgaaa tttactagtg atggtatgct aacgttgcca ggttgttttt
    16501 ttggttcatg ggaagatggg aaacttgcta ggtttgaaca atcaacggta caagtatata
    16561 aagattttac tgttagaggt actaaaaact caaccgtacc aacagaacat tatggacaac
    16621 gactattaaa cgcttatgaa actccagaat attatttcgc tgattatggg gaagccgtta
    16681 caggtgatga tggtaaagtt cgtgttgata ttgaccccat gtttgctgag acagtaaatc
    16741 taagtcgata tatgacacat gtgacaccta cagaactagt tttgtgtgct gttactcatg
    16801 aagatattga ccatttcatc attgaaacta gtaagccaaa cgtattagtt agatggaatt
    16861 tagtggcaca ccgtctaggg tatgaagata ttagattaaa agaggataca gcatatgata
    16921 gcacagtgct tgaccaaaaa cgtttttaaa acgaagacaa ggaggtatat aaatggctag
    16981 cagtttatat aatttggctc tagatttcag caaagaatta aactacacca aagctattat
    17041 ggctcgtcaa ggtgataaag ggattacagt gactgttaaa ccatttttaa atggcttgca
    17101 gatggatacg agtggcggaa catttacttt aaaaggaaca acgccatcta accgttacgt
    17161 agatagtgtg gcaactagtg taactagtga agaagtcacg ttttctcttg atggcacatt
    17221 tatgagtgaa gcaggatatt ataaacactg ctacgtagaa tatagaaaag acaatcaaat
    17281 tttaacaacg caagatatca tttttttctc actaggagtg tctgacattt cgcaaggcca
    17341 agccgatgaa tacgtttcgc aattggaaga gttgattcaa aagtataacg aaacttttga
    17401 tgcttttatg gctgaaatcg aaggtagagt ggacagctta aataaacaga ttactgattt
    17461 aactggtcaa gctaaaacac tacaagacaa gttagacgct ctgaaagaag aaatttctaa
    17521 attaggtaac ttgcaggtga tgtacagtaa cagcatcga
//
LOCUS       DACEEZ010000057        17336 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20313_2017
            SAMN12639966-rid9879223.denovo.057, whole genome shotgun sequence.
ACCESSION   DACEEZ010000057 DACEEZ010000000
VERSION     DACEEZ010000057.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639966
            Sequence Read Archive: SRR10023158
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 17336)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17336)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741683.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741683.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:44:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,016
            CDSs (total)                      :: 2,962
            Genes (coding)                    :: 2,889
            CDSs (with protein)               :: 2,889
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 36 of 73
            Pseudo Genes (incomplete)         :: 29 of 73
            Pseudo Genes (internal stop)      :: 18 of 73
            Pseudo Genes (multiple problems)  :: 8 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17336
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639966-rid9879223.denovo.057"
                     /isolate="E20313_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(<1..188)
                     /locus_tag="IWO89_001922"
     CDS             complement(<1..188)
                     /locus_tag="IWO89_001922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAQ0456653.1"
                     /translation="MRKYTFVFKKKVVSDYLNNEGGYKYLAHKYQINRTLVRHWVRIY
                     NYHGWEGLVGGGKSYTTK"
     gene            532..939
                     /locus_tag="IWO89_001923"
     CDS             532..939
                     /locus_tag="IWO89_001923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0456654.1"
                     /translation="MSYRVQLIISEDVESQQFGTNVINKVINPALSINAPLIPTALSF
                     SVTAVVSEIEDTEKIKIVEIEVLNKNEKQIFSTGEVSVSLPPQVNDINFNINARNVLV
                     EEAGEHYAVVKFNGTEIGRQIFDIKVNKPVEKN"
     gene            953..1354
                     /locus_tag="IWO89_001924"
     CDS             953..1354
                     /locus_tag="IWO89_001924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0456655.1"
                     /translation="MLDIVPSNTHRSGSLINFAAIFACASVILASPVVVDASSTPKTK
                     NDNQLVITTKNEINTISSNDGKIFDVINTVLKRKAEINIKYDEDLNLYFFVIKTTSEL
                     FSSDYDVLDTLDNVLSDYKYMGKSVVATLGE"
     gene            1356..1727
                     /locus_tag="IWO89_001925"
     CDS             1356..1727
                     /locus_tag="IWO89_001925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0456656.1"
                     /translation="MFDFNEYLSISKQIESDDKYNSKESYRRTAVSRAYYSAFKKSDE
                     YLKENYDIIYNGSSGKGSHQTVWNLFSTVKELNTLGIHNSGYRMLEKRKCADYIPNET
                     ITKTDMALMNREAEKIINKLT"
     gene            complement(1763..2062)
                     /locus_tag="IWO89_001926"
     CDS             complement(1763..2062)
                     /locus_tag="IWO89_001926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0456657.1"
                     /translation="MLFNETQLWFKFDPTNRFIKDFYKVWNSEVFFLAIEDSLLINLY
                     YSNKNYFKIPAAKTRMKKGVYFLFDIVTDVPDARSDHRRYDYIKYTFVDPERYKD"
     gene            complement(2068..2298)
                     /locus_tag="IWO89_001927"
     CDS             complement(2068..2298)
                     /locus_tag="IWO89_001927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002378238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0456658.1"
                     /translation="MMEDFIRKNISDEYADFYEQSSKKDKFQMDVSILAILAFSENKQ
                     PVTAKKETVFSEGKIKTRYILEVETNFKNRSE"
     gene            complement(2674..3810)
                     /locus_tag="IWO89_001928"
     CDS             complement(2674..3810)
                     /locus_tag="IWO89_001928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HAQ0456659.1"
                     /translation="MWIETKTDKNGKKVYKYNERYIDPKTRKRKKVSITYKNKSRETQ
                     KVALLELNKKIDIKLNEKTLQKPDLTFHELVEEWLVIYKRQVKESTYYPTNNILNTIK
                     KKIPETYIVSGINTVDLNNIFEDMIYKDDLSNKYVSVIKSKLNLLFSYAMKKGYVEKN
                     PINEVVIDYKRESKTIKIKDKFLEDDEYKRLVNFTTSHNKRYSLLFQWLYLTGMRPGE
                     AIALSKDDVHITNNNASVVINGTMMYRERSIADMKKSDSTKTAAGMREIDLPKKAIAI
                     YNELLELNPNGQFLFQTTKGTPFQLTAINTYLRNHKADMKIDKKLSSHIFRHTHISKL
                     AELGTPLYAIQDRVGHENSDITEKIYLHVTKGVKEKLKEDIEKL"
     gene            complement(3926..4840)
                     /locus_tag="IWO89_001929"
     CDS             complement(3926..4840)
                     /locus_tag="IWO89_001929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4352 domain-containing protein"
                     /protein_id="HAQ0456660.1"
                     /translation="MKKIVFGLLFLGLVLSGCSTSPNSASTKENTVSSTSEYETKKLT
                     SSDVHKIKVGDDLTQVYQTLGSPLRKWTNDFVYKELDNAITRDELIVSISKDAQGDYY
                     KEHSELIEHGNSAKDIKKLEMLQYTYSTDSDDETMLIWVSPKTEQVVYYNQRTFLDKD
                     GKSKDSSSTTTTTTTSSSEPQTASVAHSIGEAVPFTSENGNSINVTVQSVSKDYGDDF
                     YKPEGLFYAKVDFLVENTGTTPFDASSSYLEFYDSSDIKSDLASRDYFSETIQPGKSA
                     QGTAYFEVKNDGTTFEVFFADTSWKGSY"
     gene            complement(4931..5359)
                     /locus_tag="IWO89_001930"
     CDS             complement(4931..5359)
                     /locus_tag="IWO89_001930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ImmA/IrrE family metallo-endopeptidase"
                     /protein_id="HAQ0456661.1"
                     /translation="MEMDTINLVEELKRKYQSANPFYICEKMGIKIQYVPFIENPKGQ
                     FQEIRGRAIIFLNDELRDSEERFYICAHELGHAIFHRGLSSYYVSTRTSRSKSESEAN
                     CFAANLIVSLYKEDNDQYPKRIEELKNLYGLPESAYRFLI"
     gene            complement(5364..5738)
                     /locus_tag="IWO89_001931"
     CDS             complement(5364..5738)
                     /locus_tag="IWO89_001931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAQ0456662.1"
                     /translation="MNLLDRIKELAHKRGISITQLEEELNIPKNTIYQWKNRTPSTKR
                     LQLVADYFNVTTDYLLGRNQVPDWATKDEVVELDKLLDSNVNMSYGGETLTPEQVQRV
                     KDILIGTFWDIVKEDKEKGKKM"
     gene            6024..6209
                     /locus_tag="IWO89_001932"
     CDS             6024..6209
                     /locus_tag="IWO89_001932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAQ0456663.1"
                     /translation="MSENLDLKIRAEMRKRRMTFKELAALIGISGAYLSDILNGNRDG
                     KKAQQHIETVKKILDIR"
     gene            6221..6469
                     /locus_tag="IWO89_001933"
     CDS             6221..6469
                     /locus_tag="IWO89_001933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0456664.1"
                     /translation="MTKLKKQDFVKKYNYSPSTYQRRMSELKNTAIFSAAYERVTGQE
                     VWINTELYDKFLSFKSYNRLRTRKVTPKEFIEKHLVDL"
     gene            6500..6679
                     /locus_tag="IWO89_001934"
     CDS             6500..6679
                     /locus_tag="IWO89_001934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0456665.1"
                     /translation="MGKFNRALVFSAPLIIYALGLWGSRQALIGTIVYMVWIFMGLDE
                     AEAEYRAKKPTEGAD"
     gene            complement(6722..7192)
                     /locus_tag="IWO89_001935"
     CDS             complement(6722..7192)
                     /locus_tag="IWO89_001935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0456666.1"
                     /translation="MSIISFILSTFLTGIKIKGMRPQLEVELNASYFAADMIYTKVII
                     SNYSTEPAMLVNLELSSANLNRRWSATPFKKLIAKGGSVDDNRIYSEAVPLNIPPKSA
                     ISCYLAFEVGKINFNKLLNHQTKMIFTLNRTQIHKIVDIKNTNFPIEKLVKELN"
     gene            7279..7977
                     /locus_tag="IWO89_001936"
     CDS             7279..7977
                     /locus_tag="IWO89_001936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage regulatory protein"
                     /protein_id="HAQ0456667.1"
                     /translation="MQELVILKNKEAVTTSLQVAESFEKKHQHVLRDIDALKKDVSNF
                     GQMFVEGNEPDSYGRNRRVFFISRDGFFLLAMGFTGKKAIYFKQKYIEAFNEMEDVIR
                     KNTVPQTIEDMMIYQLEEMKDVKKDVSMLKDTMRISGQQEFEIKQKGNMKVMGVLGGK
                     ESRAYEEISKKVFSKFWSEFKRTFSIPRYGELPRKRFDDAVSFIEMWLPETAIRMEID
                     QLNRQQRLFGDDNE"
     gene            8155..8496
                     /locus_tag="IWO89_001937"
     CDS             8155..8496
                     /locus_tag="IWO89_001937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0456668.1"
                     /translation="MEKELSTLDQYLIDPDWGKPKIEETSGRKIRRNLLTNEELAWDQ
                     DDLGNHVTIWDHVYLIHLSKHSNKPEYIYVIEDGLIDALEEYDRDNLIDISYYGPGKK
                     YIAEMEAEFDE"
     gene            8489..9160
                     /locus_tag="IWO89_001938"
     CDS             8489..9160
                     /locus_tag="IWO89_001938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1071 domain-containing protein"
                     /protein_id="HAQ0456669.1"
                     /translation="MSEIKGTTNFEKLFSRKLNKILKKKGNFDYLSWAHAWEIMKKND
                     PQATVTINEYKHYRVVSGTHQDFLVEEYKPFLMDETGTYVSVSVTVKGHTETELFPVL
                     DYRNQPVVKPNAMQINNSLKRCFVKALALHGLGLYVFQGEDIPTPPRIDTKKLNMLET
                     ILEAFNEQMGKDMTKTLIEYVNEQTDKLGLLADNVETIEQLSYEQCALMERAIAAKRK
                     ELDKK"
     gene            9166..9852
                     /locus_tag="IWO89_001939"
     CDS             9166..9852
                     /locus_tag="IWO89_001939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0456670.1"
                     /translation="MFKPLIDSYSAVLKKFKGKDISATINEEVNIDRLKTMYDGYDGD
                     RVIEIRFIDPRRFTVQQRNFIYALIGDIFIDTGMPTDFWKEFFYFRFEGVTGRKISLK
                     DESNTTVSDANVLANIILDFIFEHHIPFKEGYEILPANQEYYFYKCITKRVCCICGKT
                     GADIDHFDKALGRRKRKEVDHSEYTFAALCRIHHTEKHKIGVINFKNKYQIKGIKLSH
                     ETIKKLRIGG"
     gene            9855..10685
                     /locus_tag="IWO89_001940"
     CDS             9855..10685
                     /locus_tag="IWO89_001940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein"
                     /protein_id="HAQ0456671.1"
                     /translation="MAEISWIKLKTTMFDDEKIRLIQAVPESDAIIVIWIRLLVLAGK
                     TNDDGLIYIQRNMPYTEEMLATLFGKNVNTVRLALTTLANFNMIDLSSDGLIAISNWE
                     KHQNIEGMDKVRLKNAERNRKYRERKRQERLKLENDVSVTSRDGTDKDIEEDKDIDKE
                     EKKGKYSDEHLRLAKKMQRNLTEDFPKEMNKVDIEKWADTIRLMEERDKASIEAIEYV
                     INWLPTNEFWFGNIRSAKKLREKFEKLKFEIKADKKNHKKQSQKLQYSNPSEYDDLPI
                     "
     gene            10701..11552
                     /locus_tag="IWO89_001941"
     CDS             10701..11552
                     /locus_tag="IWO89_001941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HAQ0456672.1"
                     /translation="MESLANAMEKLIRRVLVQSGKCPECSEPLYSWRAKNKDGSERCK
                     PTCMSCGYKALRVKEDIQTERIYNDSLKARALSFFQNGSVLTDKTLFKCKMENYHVVD
                     QETKIALERAKSYVNDVLLNHPAHFILSGKSGSGKSHLSMATAWEILERSNYDKKILF
                     ISYQELLEQIKFSYNNAELRKEIEGSLIADIKTTDLVVFDDIGAELGSGVSNSRQFTN
                     NTLNTLLEARQNKATIITTNLSGPELREAYGERIVSRIFKNSEGYALKFQQTADKRIK
                     PVKGSIA"
     gene            11549..11908
                     /locus_tag="IWO89_001942"
     CDS             11549..11908
                     /locus_tag="IWO89_001942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1064 domain-containing protein"
                     /protein_id="HAQ0456673.1"
                     /translation="MNKYRNKKTVHRGIKFDSIAEAEYYDLALWQAEANGWKVKLQER
                     FELMPKFELDGKKYRKIEYIPDFTFYKNGKLVKVVDVKGMQTKDFKIKAKLFCSQYRV
                     PLILAKKYRNTFKEERF"
     gene            11923..12084
                     /locus_tag="IWO89_001943"
                     /pseudo
     CDS             11923..12084
                     /locus_tag="IWO89_001943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752308.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin"
     gene            12081..12386
                     /locus_tag="IWO89_001944"
     CDS             12081..12386
                     /locus_tag="IWO89_001944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0456674.1"
                     /translation="MSVDKKAAMKRIIELTHSENWQEDKEIVAEVQKLGKSMWAEKPK
                     RKTPRKIAIWHGDRILVTGTAEQLSEITGLSKNIIWDRARSLWIDSKGRQFRYVEEK"
     gene            12386..12697
                     /locus_tag="IWO89_001945"
     CDS             12386..12697
                     /locus_tag="IWO89_001945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0456675.1"
                     /translation="MNELITKVEQWAKDKGLDQADPKAQFLKVAEEFGEIASAMARSN
                     DELFKDSVGDVIVTLIILSMQKGANIQECLEMAYNEIKGRTGKMVDGVFVKSSDLEDS
                     E"
     gene            12864..13280
                     /locus_tag="IWO89_001946"
     CDS             12864..13280
                     /locus_tag="IWO89_001946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-packaging protein"
                     /protein_id="HAQ0456676.1"
                     /translation="MIPRFRAWDKRKNVMRDVAVLHFTKNGKTNFIEYWINPTELKSY
                     HVRNIELMQSTGLKDKNGIEIFEWDIVSVSVRNGFDYLDNKVCVVKNSIGHSGLVCAT
                     VDEDLEYQIFNTELFEEYTYEVIGNIWENSELLEEQ"
     gene            13280..13948
                     /locus_tag="IWO89_001947"
     CDS             13280..13948
                     /locus_tag="IWO89_001947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1642 domain-containing protein"
                     /protein_id="HAQ0456677.1"
                     /translation="MNKQELIDELAKYVKRYENVMDEHGQGRYGAYEVSLKLVKRLNE
                     SKITDEQAWNKVAEAYPESAQSLRNTLDNAVFGKTGEPQKHVMPKFVAEWIEYAKKKG
                     DSLAISFKPWNLYGVEYSKADRWIGDNQETFARAWLDGYEVEKEPLYYVPLPYEVWDE
                     EAAELKTEYLYLHYEITSDETRIFPTKEPRKGFVAKLDELTIKSADENYWPFAVPVEE
                     VVEG"
     gene            13945..14322
                     /locus_tag="IWO89_001948"
     CDS             13945..14322
                     /locus_tag="IWO89_001948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0456678.1"
                     /translation="MKKLHQLISEKESELQNLEDSLGLGFPIVEQVKMVQISHLQLEL
                     EDLRQIEDPYQLNDNQQIVLEWLKLTASTGKPMQVVFWMMNNAAWGHLDELRDPLMEL
                     TDKQQFEVLTAFAQWGLEQEEKE"
     gene            14319..14618
                     /locus_tag="IWO89_001949"
     CDS             14319..14618
                     /locus_tag="IWO89_001949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0456679.1"
                     /translation="MKLKDGFYASSHGIGGLMLDMPTKNPKTRKKPKVKVGDMVRCEA
                     EGFIYPFRGYVEHVYNHSAIIRIENTMECDKLLAKSKDNLAVVRLVDMEVINNEI"
     gene            14860..15276
                     /locus_tag="IWO89_001950"
     CDS             14860..15276
                     /locus_tag="IWO89_001950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autolysin"
                     /protein_id="HAQ0456680.1"
                     /translation="MQLLREVDFKQTRCNARDVLKNFRRLERMAGRSLIDIKSPIITD
                     MPKAPKHGNKAEDAIIQMMDIEAERDAILAALMALSLISRQILYYSFCDVNKHSNYEI
                     GQLIRGYGEKNVEKLKSIALIEFAEAYKKGVLVQYR"
     gene            15628..16032
                     /locus_tag="IWO89_001951"
     CDS             15628..16032
                     /locus_tag="IWO89_001951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605-like element ISEfa4 family
                     transposase"
                     /protein_id="HAQ0456681.1"
                     /translation="MELDTNNHSVFLLYYHLVLVTKYRRQVIDDEISDYAKTTFERIS
                     ESYHITLVEWNHDKDHVHIMFKAQPKTELTKFINAYKSASSRLIKRDFPRVKQFLWKE
                     MFWSKSFCLLTTGGAPIDVIKKYIQNQGNNHK"
     gene            16049..17197
                     /gene="tnpB"
                     /locus_tag="IWO89_001952"
     CDS             16049..17197
                     /gene="tnpB"
                     /locus_tag="IWO89_001952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family element transposase accessory
                     protein TnpB"
                     /protein_id="HAQ0456682.1"
                     /translation="MEQLKAYKFRIYPTEEQEIFFAKSFGCVRKVYNLMLDDRKKAYE
                     EVKNDSSKKMTFPTPAKYKKEFPFLKEIDSLALANAQLNLDKAYKNFFRDKSVGFPRF
                     KSKKNPVQSYTTNNQNGTVALIDSKFIKVPKLKSLVRIKLHRQPKGIIKSATISRHSS
                     GKYYISLLCKEEIIELPKTNSTIGIDLGIMDFAILSDGQKIDNNKFTSKMEKKLKREQ
                     RKLSRRALLAKNKGTNLFEAKNYQKQKRKVARLHEKVMNQRTDFLNKLSTEMIKNHDI
                     ICIEDLNTKGMLRNHKLAKSISDVSWSKFVTKLQYKADWYGRKIIKVDKWFPSSQICS
                     ECGHKDGKKSLEIREWTCPVCHTHHDRDINASINILTEGLRLHSMGLA"
BASE COUNT         6079 a         2655 c         3382 g         5220 t
ORIGIN      
        1 aattttgtag tgtagctttt gccacctcca accaagcctt cccaaccatg atagttataa
       61 atccttaccc aatgtctaac cagtgtacga tttatttgat atttatgtgc aagatatttg
      121 tagccgcctt cgttatttaa atagtctgaa actacttttt tcttaaaaac aaatgtatat
      181 ttccgcaaaa aaagcacccc ttttaattag atttctagtc taacttttgg ggtgcacatc
      241 atttttgagg ggaagtacat atagttgttg atatagttgc taatattgtt agctaatttg
      301 ttagtaaaac tgtatacgaa tttagatagt aaaaaaacag tttgttcctt atatagaggc
      361 acttttacta ctgattaaat gtcagatatg cttacaaata tacttataaa tatgcttaca
      421 aaattagcta atatttccgt tgacaaagct ttgttactat tgttaaacta aattgactag
      481 caaaagtgaa acataaattt attaaataaa tgtaaaaatt aggagaaatc aatgtcttat
      541 agagtacaat taataatttc agaagatgta gaaagtcagc aatttggcac taatgttatt
      601 aataaagtaa ttaatccagc tctttctatt aatgcgcctt taattcctac tgctttatca
      661 ttttctgtaa cagctgtggt gtctgagata gaggatacag aaaagataaa aatagtagaa
      721 attgaagttt taaacaagaa tgaaaaacaa attttttcta cgggtgaagt atcggtaagc
      781 ttgcctcctc aagttaacga tataaacttt aatataaacg ccagaaatgt cttggttgaa
      841 gaagcaggag agcattatgc tgttgttaaa tttaatggaa ctgagattgg aaggcaaatc
      901 tttgatatca aggtcaacaa accagtggaa aaaaattaag gagacgatac ggatgcttga
      961 tatagtacca tcaaatacac atcggagtgg tagcttaata aattttgctg cgatttttgc
     1021 ttgcgcatcg gtaattctag catctccagt tgttgtggat gcaagttcta ctccgaaaac
     1081 taaaaatgat aatcaacttg taattactac aaaaaatgag attaacacaa tttcaagtaa
     1141 tgatggcaaa atttttgatg tgataaatac ggttttaaaa agaaaagctg aaatcaatat
     1201 aaaatatgat gaagatttaa acttgtattt ctttgttatc aaaacaacat ctgaattgtt
     1261 ttcctcagat tacgatgttt tggatactct cgataatgta ttatctgact ataaatatat
     1321 ggggaaatct gtagtagcaa ctttggggga ataaaatgtt tgactttaat gaatatttat
     1381 ctatttccaa acaaattgaa tcagatgata aatataattc taaagaatct tatagaagaa
     1441 ctgctgtttc aagagcatac tacagtgctt ttaaaaaatc tgatgagtat ttgaaagaaa
     1501 attatgatat tatttataat ggaagtagcg gaaaaggaag ccaccaaacc gtttggaatt
     1561 tgttttcaac agttaaggaa ttgaatacct taggaataca taatagtggg tatagaatgt
     1621 tagagaaaag aaaatgtgct gactatattc ctaatgaaac cattacgaaa acagatatgg
     1681 cgctgatgaa tcgagaagca gaaaaaataa taaacaaact cacctaatga atttcaagcg
     1741 agtttgtttg ttattttttt atttaatctt tgtatctttc tggatcaacg aaagtatact
     1801 ttatatagtc ataacgccga tgatcgctac gtgcgtccgg cacgtcagtc acgatatcaa
     1861 acaaaaagta tacacccttc ttcattctag ttttcgcagc aggaattttg aaatagttct
     1921 tattagaata gtagagattg attaataagc tatcttcgat tgctaaaaag aaaacttctg
     1981 aattccatac tttataaaaa tctttgataa atctattcgt aggatcgaat ttaaaccata
     2041 attgtgtctc attaaaaagc ataaccatta ctccgatctg tttttaaaat tagtttctac
     2101 ctctaatata tatcgagttt ttattttgcc ttcagagaat actgtttctt tttttgcagt
     2161 tacaggttgt ttattttcgg aaaaagctaa tatagctaaa attgaaacat ccatctggaa
     2221 tttatctttt ttgctgcttt gctcataaaa gtctgcatat tcatcactga tattttttct
     2281 aataaagtct tccatcataa aaatcacctc aaaacgatta tacgaactta cgttctcgtt
     2341 gtaaagcgat atttgagggg caaaaagggg gcaaaaatac gtttatcttt gatatatctc
     2401 agattgaaca tagtgtttat tatatgtcgg tattgttgat ttatagctgt tttgagggta
     2461 ttagatacat aatagaatat agtggttttg tttacgtgca ttacacggaa aagctgctcg
     2521 tatcaaagaa atccgtcgtt aatcatcgtt atcagactga gaaagaaccc ttggtattca
     2581 agggttcttt ttttgttctc tgttttttta tattaacgat ttctatttgg gacatttttg
     2641 attaataaaa aggggcaaaa aaggggcaac aagttacagt ttttctatat cttctttcaa
     2701 tttttctttt actccctttg tcacatgcaa ataaatcttt tcagtgatat cactattttc
     2761 gtgcccaacg cgatcctgaa tagcatacag aggagttcct agttccgcta attttgaaat
     2821 atgggtatgt ctaaaaatat gtgaactaag ttttttatca atcttcatgt cagctttatg
     2881 atttctcaaa taggtgttaa ttgctgttag ttggaaagga gttcctttcg ttgtttgaaa
     2941 tagaaattga ccattcggat ttaattctag aagctcattg taaatagcta tcgctttttt
     3001 tggtaaatca atttctcgca ttccagcagc agtttttgta gaatcagatt ttttcatatc
     3061 agctattgaa cgttctctat acatcatcgt cccatttata actactgatg cattattatt
     3121 ggtaatgtgt acatcgtctt tacttaatgc gattgcttct ccaggcctca ttccagtcaa
     3181 atatagccac tggaaaagga gagaatatct tttattgtgt gaagttgtga aatttactag
     3241 tcttttatat tcatcatctt ctaaaaactt atctttaatt tttatcgttt ttgactctcg
     3301 tttataatca ataactactt cgttgatagg atttttttct acatagcctt ttttcatagc
     3361 gtatgaaaaa agaagattca atttggattt aattacactg acgtacttat ttgacaagtc
     3421 atctttatat atcatatctt caaaaatatt atttaaatct actgtattaa taccagaaac
     3481 gatgtaagtc tcaggtatct ttttctttat agtgtttaag atattattcg taggataata
     3541 tgtagactcc ttaacttgtc ttttgtaaat aactaaccat tcttcgacaa gctcatgaaa
     3601 tgttagatca ggcttctgaa gtgttttttc gtttaatttt atatcaattt ttttatttaa
     3661 ctctagcaat gccactttct gagtttctcg agatttattt ttataagtaa tgctcacttt
     3721 ttttcttttt ctagtttttg gatcaatata tcgctcatta tatttatata ctttttttcc
     3781 gtttttatca gttttggttt caatccacat tgttttcatc tcctatttgt tgctataata
     3841 ggaatggata agtaagccaa ttatagcagg tttatttttc atcacgtcca caaactttgg
     3901 cgagggaggg ggcgtgtttt tttgtttagt agctaccttt ccatgaagta tctgcgaaga
     3961 acacttcaaa tgttgttcca tcatttttca cttcaaaata tgctgttcct tgtgcggatt
     4021 tacccggttg gatggtttcc gaaaagtaat ctctactagc taaatcagat ttaatatctg
     4081 atgaatcata aaattctaaa taacttgaac ttgcatcaaa aggtgtagtc cctgtgtttt
     4141 ctacaagaaa atctaccttt gcgtaaaata ggccttctgg tttatagaaa tcatcaccgt
     4201 aatcttttga tacagactgg acagttacgt taatagagtt accattttca gaagtaaaag
     4261 gaactgcttc tcctatacta tgtgcaactg atgccgtctg gggttctgaa gacgaagttg
     4321 tagttgtagt tgtagtagag cttgaatctt tggattttcc atctttatct aagaaagttc
     4381 gttgattata atatacaacc tgttcagttt ttggactaac ccaaattaac attgtttcat
     4441 catcagaatc agttgaataa gtatattgga gcatctctaa tttttttata tcttttgcac
     4501 tatttccatg ttcaattaat tcagagtgct ctttataata atctccttgt gcatcctttg
     4561 aaatagatac aattaattca tcccgtgtga tagcattgtc taattctttg taaacaaaat
     4621 catttgtcca tttacgtaac ggtgaaccaa gtgtctgata aacttgagtt aaatcatcac
     4681 caacttttat tttatgaacg tctgatgaag ttagtttttt tgtttcatat tcagatgttg
     4741 atgaaacagt attttcttta gtgctagcag aattaggact agtggagcat ccgcttaata
     4801 ccaagcctaa aaaaagcaac ccaaaaacaa tttttttcat ttttaattcc tcacttctat
     4861 gatatgattt ttttgtagga tcttagaaat aaggttttta gtccgtgttg cagcacggac
     4921 tttttttaca ttatataaga aagcgataag cgctttctgg aagtccgtaa agattcttta
     4981 attcctcaat tcgtttagga tattgatcat tgtcttcttt gtatagagaa acaatgagat
     5041 tagcagcaaa gcaattagct tcgctttcag atttgcttct agatgttctt gttgatacat
     5101 agtaactgga taagccacga tgaaaaatag cgtgacctaa ttcgtgagcg caaatgtaga
     5161 atctttcctc agagtctctc agttcatcat ttaagaagat tattgcacga cctctaattt
     5221 cttgaaactg tcctttggga ttctcgataa aaggaacgta ttgaatttta atgcccatct
     5281 tttcacaaat ataaaaagga ttagcggact ggtatttccg cttcaactcc tcgactaaat
     5341 taatcgtatc catctccata agctcacatc tttttgcctt tttctttgtc ttctttcaca
     5401 atatcccaga aggttccaat aaggatatct tttacgcgtt ggacctgttc aggcgtcagt
     5461 gtttctccgc cataagacat attgacattt gaatctagta atttatcaag ttcaactact
     5521 tcatcttttg ttgcccagtc aggaacttgg tttctaccga gtaaataatc agtagttaca
     5581 ttaaaataat ctgctacaag ttgtagtctt ttagtactag gagttctatt tttccattga
     5641 taaatcgtgt ttttaggtat atttaattct tcttccaatt gagttatact tatgcctctt
     5701 ttatgagcta attcctttat tctatctagt aaattcattt tcgcacctca caagctacga
     5761 aaacactaat aaaaaaatta gttaaaggtg ttgacaacta ataaaagtgt tagtatactg
     5821 ttttcgtaag ctaaaatatt agctaaaaag acttgataac taataaacac ttcacggtcg
     5881 gcaaactctg aaatgtaaat tactaggcat ttctgtgtct tatttagcta tgtctatata
     5941 ctaataaaaa tattagttat tgtcaacgaa tattagctaa ttttttagct tacagattat
     6001 ttttttagaa aggagctatt tttatgtctg agaatttaga cttaaaaatt cgagcggaga
     6061 tgagaaaaag aagaatgact ttcaaagaac tagctgcgct tatcggtatt tcaggagctt
     6121 atttatcaga tattctaaac ggcaatcgtg atggaaagaa agcacaacag catatcgaaa
     6181 cagtgaaaaa aatattggac atccgatagg ggggtaggcg atgacaaaac taaaaaaaca
     6241 agattttgta aaaaaataca attattctcc atctacttat caacgtcgaa tgtcggaact
     6301 aaaaaataca gcaattttct cagcggcgta tgaacgggtc acaggacaag aagtttggat
     6361 caatacagaa ttatacgata aatttttgtc tttcaaatcc tataacaggt tacgcacaag
     6421 aaaggtaaca cctaaagaat ttatcgagaa gcatttagtt gatttataaa aaacagaaca
     6481 ttttgagagg tgaaggttaa tgggtaaatt caacagagca ttggtattca gcgcaccgct
     6541 aatcatctac gctttaggac tttggggaag caggcaagcg ttgataggaa cgattgttta
     6601 catggtttgg atttttatgg ggcttgatga agctgaagct gagtacagag cgaaaaagcc
     6661 aaccgagggg gctgactaaa aatgaataga aaagagaaac tagaatgtat attactatta
     6721 ctcagtttaa ttctttcact aatttctcta ttgggaagtt tgtatttttg atatcaacaa
     6781 ttttgtggat ttgggttcta tttagagtaa atatcatttt tgtttggtga ttaaggagtt
     6841 tattaaaatt aattttccct acttcaaatg caaggtaaca agatatagca cttttaggag
     6901 gaatatttag cggtacagct tcagagtata ttctattatc atcgactgat cctcctttag
     6961 caattaattt cttaaatgga gtagctgacc atctgcggtt taagttagcg gatgaaagtt
     7021 ctaaattaac taacatagct ggttctgttg aatagttaga aatgattact tttgtataaa
     7081 tcatatctgc agcgaagtaa gaagcattaa gttcaacttc tagttgaggg cgcattcctt
     7141 ttatttttat ccctgttaaa aacgtactca atataaaact tattattgac atccattgaa
     7201 aaattgttaa gtgaaaaaaa ttcaaaataa acaccaccag tttttaacta aattatacca
     7261 aaaaggagag aagaaataat gcaagaatta gtaattttga aaaataaaga agctgtgact
     7321 acgagcttac aagtggcaga aagcttcgag aaaaaacatc aacatgtttt aagagatatt
     7381 gatgcactaa aaaaagatgt gtccaatttt ggacagatgt ttgtagaagg taatgaacca
     7441 gattcatatg gcagaaatcg acgagttttc ttcattagta gagatggatt tttcttgctg
     7501 gctatggggt ttacaggaaa gaaagctatc tacttcaaac aaaaatacat tgaagcattc
     7561 aacgaaatgg aagatgttat tcgcaagaat actgttcctc aaacaattga agatatgatg
     7621 atctatcaat tagaagaaat gaaagatgtt aaaaaagatg tttccatgct taaagatact
     7681 atgcgaatta gcggacaaca agagtttgaa attaagcaaa aaggaaatat gaaagttatg
     7741 ggagttctag ggggaaaaga aagccgagct tatgaagaaa tcagcaaaaa agtattctca
     7801 aaattttggt ctgaatttaa acgtaccttt tcaatcccaa gatatggcga gttacctcgt
     7861 aagagattcg atgatgctgt ttcatttatt gaaatgtggt tgccagaaac tgcgatccgt
     7921 atggaaattg atcaactgaa cagacaacag agactttttg gtgatgacaa tgaatagagc
     7981 tgaagcgcta agaataggga cggcaattgc taattgctgg tggaaatact ataaaccaat
     8041 catcctaagc caacaacata ttgacaagca aaaagcatgg caacaaataa aaaagtgact
     8101 ccgccggcaa gcaaagagtc acaaagaaaa cacatcataa ggagatttta gcatatggaa
     8161 aaagaacttt ccactctaga tcaatatttg attgatcctg attggggcaa gccgaaaatt
     8221 gaggaaacaa gtggtcgaaa aatcagacga aatcttttga cgaatgaaga actagcttgg
     8281 gatcaagatg atttaggcaa ccatgtaact atttgggatc atgtttatct tatccatcta
     8341 tcgaagcatt cgaataaacc tgaatatatt tacgtcatcg aagatggctt gattgatgcg
     8401 ctagaagagt acgacagaga taacttgatt gatatctctt attacggacc aggtaagaaa
     8461 tacattgctg aaatggaggc agaatttgat gagtgaaatc aaagggacaa cgaactttga
     8521 aaaacttttt agtcgtaagt taaataaaat tctcaagaaa aaaggaaatt ttgattattt
     8581 atcttgggct cacgcgtggg agattatgaa aaagaatgat ccacaggcaa cggtaactat
     8641 taatgagtac aaacactaca gggttgtttc tggaactcat caagactttc ttgttgagga
     8701 atataaacct tttcttatgg atgaaactgg gacttatgta tctgtctcag taacggttaa
     8761 aggacacacg gaaaccgaat tatttcctgt tttagattat cgaaaccaac cagttgttaa
     8821 accaaatgca atgcaaatca ataactcatt gaagcgatgc tttgtgaaag cattggctct
     8881 acacggactg ggattatatg tatttcaagg ggaagatatt ccaacaccac ctagaatcga
     8941 tacaaagaaa ttaaacatgc tagagacgat tctagaagct ttcaatgagc agatgggtaa
     9001 agatatgacc aaaaccttaa tcgaatatgt taatgagcag acagataaat tagggctctt
     9061 agctgataac gttgaaacta ttgaacagtt aagctatgag caatgtgcct tgatggagcg
     9121 agcaatagca gctaagagaa aagaattaga taagaagtga tatgagtgtt taaaccatta
     9181 atcgattcat attcagcggt tctgaaaaag ttcaaaggaa aagacataag cgcaaccatc
     9241 aatgaggaag tgaacattga tcgactaaag acgatgtatg acggctacga tggtgatcga
     9301 gtcattgaaa ttcgttttat tgatcctaga cgtttcaccg tacagcaacg aaacttcatc
     9361 tatgcgctga taggcgatat ttttatcgat acaggcatgc caacggactt ctggaaggaa
     9421 ttcttctact tccgttttga aggtgtcaca gggcgcaaaa taagcctcaa agatgaatcg
     9481 aatacgactg tgagtgatgc taatgtctta gcaaatatca tcttagattt catctttgaa
     9541 catcatattc ctttcaaaga aggctatgag attttaccag cgaatcaaga atattacttc
     9601 tacaaatgca ttacaaaaag agtttgttgc atctgtggca aaacaggagc tgacatcgat
     9661 cactttgaca aagcgctagg aagacgaaag cgaaaagaag ttgatcattc agagtacaca
     9721 tttgcagcac tctgcagaat ccatcacacg gagaagcaca aaataggtgt gatcaatttt
     9781 aaaaataaat atcaaatcaa aggaatcaag ttaagtcatg aaacgattaa aaagttaagg
     9841 ataggaggat aaatttggct gagataagtt ggatcaaact taaaactact atgtttgacg
     9901 atgaaaaaat acggttaatc caagctgttc ctgagtcgga tgccatcatc gttatatgga
     9961 ttcgattact agttttagca ggaaagacta acgacgatgg tctgatatat atccagagga
    10021 acatgcctta taccgaagaa atgcttgcta cattgtttgg caaaaacgta aatacggttc
    10081 gcttagcgct aactacattg gcaaatttca acatgattga tctaagcagt gatggactaa
    10141 ttgccatcag taattgggaa aaacatcaaa atatcgaggg tatggataaa gtaaggctaa
    10201 aaaatgctga aagaaaccgt aaatacaggg aaagaaagag acaggaacgt ctcaaattgg
    10261 aaaatgacgt tagcgtgacg tcacgtgacg gtacagataa agatatagaa gaagataaag
    10321 atatagataa agaagaaaag aaaggtaagt attctgacga acacttacgc cttgctaaaa
    10381 aaatgcaaag gaatttaact gaagattttc caaaagaaat gaacaaagta gatatcgaaa
    10441 aatgggcaga cacaatcagg ttgatggaag aaagagataa agcatctata gaagcgattg
    10501 agtatgtgat caattggcta cctacaaatg aattttggtt tggaaatatt agaagtgcta
    10561 agaaattgag agaaaaattt gagaagctca aattcgaaat caaagcagac aagaagaatc
    10621 ataaaaagca aagtcaaaaa ctacagtaca gcaatcctag tgaatatgac gacttgccaa
    10681 tttaaaagga gatgcatcac atggaaagcc tagcaaatgc tatggagaag ctaataagaa
    10741 gagtattagt gcaaagcgga aaatgtccag aatgtagcga acctttgtat agttggcgag
    10801 ctaaaaataa ggatggttca gaacgttgta aaccaacatg tatgagttgt ggttataaag
    10861 cgttacgtgt gaaagaggat atacagaccg aacggatata taacgacagc ttaaaagcac
    10921 gagcattgag tttttttcaa aatggttcgg tattaacaga taaaactttg tttaaatgca
    10981 aaatggagaa ttatcacgta gtggaccaag aaacgaaaat tgctttagaa agagctaaaa
    11041 gctatgtaaa tgatgtccta ctgaaccatc ctgcacattt cattctatca gggaaatcag
    11101 gaagcggaaa aagccactta tcaatggcga cagcttggga aatacttgag cgctcaaatt
    11161 atgacaagaa aatacttttt ataagctatc aagagttatt agagcaaata aagttttctt
    11221 ataacaatgc tgaactgaga aaagaaattg aaggatcgct tatagccgat atcaaaacaa
    11281 ctgatttggt ggtttttgac gatattggag ctgaattagg tagcggggta tcaaatagta
    11341 ggcaatttac aaacaacacg ttaaacacgc tcttagaagc cagacagaac aaggcaacga
    11401 tcatcacaac aaacttatct ggtcctgaac taagagaagc ctacggtgaa agaattgttt
    11461 ctaggatatt taagaattca gaaggttatg cgctgaaatt ccaacaaaca gcagacaagc
    11521 gcataaaacc agtgaaaggt agtatcgcat gaataaatac cgtaataaaa aaactgttca
    11581 tcgaggtatc aagtttgatt ctatcgcaga agcagagtat tacgatctag ccttgtggca
    11641 agctgaagcg aacggctgga aagtaaaact tcaggaaaga tttgagctga tgccgaaatt
    11701 tgaactagac ggaaagaagt atcgcaagat cgagtatatt cccgacttca cattttataa
    11761 aaacggcaaa ctagtcaaag tcgtagatgt taaagggatg cagacaaaag atttcaaaat
    11821 caaagcgaaa ctcttctgca gtcaatatcg agtaccactg atcttagcga agaaatatcg
    11881 gaatacgttc aaggaagaac ggttttaacg aggtgatcca tcatgacaac agaagaagtg
    11941 attcaaatgc gtattcgaag ccttcagcgt gagattgacg atctgtaacg gacaaaggca
    12001 gtgatggtca atgaaacggc gaggaaggca atcgatttgc acatagaaaa tttaagaagg
    12061 aaaatccatc gattggagga atgagcgtgg ataagaaagc agcaatgaaa cgaatcatcg
    12121 aactgacaca ttctgagaat tggcaagaag acaaagaaat agttgcagaa gtccaaaagc
    12181 tcggtaaatc aatgtgggct gaaaagccta aacggaaaac gccgagaaaa attgcaatct
    12241 ggcatggtga tcgaattcta gtaacaggta ctgctgaaca gttatctgaa attactggat
    12301 taagcaaaaa cattatctgg gatagagcta ggagcttatg gattgattca aaaggacgac
    12361 agtttaggta tgtggaggag aaataatgaa cgaactaatc acaaaagtag agcagtgggc
    12421 taaagacaag ggactggatc aagcggatcc aaaagcacaa tttttgaaag tagctgagga
    12481 attcggagaa attgcatcgg caatggcaag aagtaatgat gagctattta aagatagcgt
    12541 aggagacgtt atcgtcacgc tgattattct ttccatgcaa aaaggggcaa acatacaaga
    12601 gtgtttagaa atggcgtata acgaaatcaa aggacgcaca ggaaaaatgg tagatggtgt
    12661 attcgtgaag tcgagtgatt tggaggacag cgaatgatga tgccaaaaga aatctattca
    12721 gatgcattaa gaggtgctag aaatcaattg aaaatggcaa aaagagcata cgaaattcgg
    12781 cccacaatcg aaaatgaacg cagagtaaaa gcaattcgcc gtagatgttc aatttacggc
    12841 gagttacaga aggaggacag caaatgatac cgagatttcg agcttgggat aaacgaaaga
    12901 acgtaatgag agatgtagcc gtcttgcatt ttactaaaaa cggcaaaaca aactttattg
    12961 aatattggat aaatcctacc gaattgaaat catatcacgt gcgaaacatc gagctcatgc
    13021 aatcaacagg gctgaaagat aagaatggca ttgagatttt cgagtgggat attgtatcag
    13081 tcagcgtgcg aaatggattc gattatttag ataataaagt ttgtgttgtc aaaaattcaa
    13141 taggacattc gggattagtg tgtgccactg ttgatgaaga tttagagtat caaattttta
    13201 acacagagct gtttgaagaa tacacgtatg aagtcatcgg aaatatttgg gagaatagcg
    13261 agttattgga ggaacagtga tgaataaaca ggaattgatt gatgaattag ctaaatatgt
    13321 aaagcgttat gagaacgtta tggatgagca tggccaagga aggtacggcg cttatgaagt
    13381 atctttaaag ttggtgaaaa gactaaatga atcaaaaatt acggacgaac aagcttggaa
    13441 taaggtagct gaggcttatc ctgaatcggc acaaagcttg agaaacactt tagataatgc
    13501 tgtatttggt aagactggtg aaccgcagaa gcacgttatg ccgaagtttg tggcggagtg
    13561 gatagaatac gctaaaaaga aaggtgatag tctagctatt tcattcaagc cgtggaacct
    13621 ctatggtgtt gagtatagca aggctgatag atggattgga gataatcaag aaacgtttgc
    13681 tcgtgcttgg ctagacggct acgaggtcga gaaagagccg ttgtattatg tcccgctacc
    13741 atatgaagtt tgggatgaag aggcagcgga gcttaaaaca gagtatttgt atctacatta
    13801 tgagatcaca agtgatgaaa ctagaatatt tcctacaaaa gaacctagaa aaggttttgt
    13861 cgcaaaatta gatgaactta cgattaaatc agcagatgaa aactactggc cgtttgctgt
    13921 accagtggaa gaggtggtag aaggatgaaa aaattgcacc aactcatatc tgagaaagaa
    13981 agcgaactcc aaaatttaga agatagtttg ggattggggt ttccaattgt tgagcaagtt
    14041 aaaatggttc aaatcagtca tctacaactt gaattggaag atttaagaca aatagaagat
    14101 ccgtatcaac tcaacgataa ccagcaaatc gtgttggagt ggttgaaact aactgcgtca
    14161 acaggaaagc caatgcaagt tgtattttgg atgatgaata atgcagcatg ggggcattta
    14221 gacgaattga gggatccttt gatggaatta actgacaaac agcaattcga agtcctaaca
    14281 gcattcgctc aatggggatt agaacaggag gaaaaagaat gaaactaaaa gacggatttt
    14341 acgctagtag ccacggtatc ggcggtttaa tgctagacat gccgacaaag aaccctaaaa
    14401 cacgtaagaa gccaaaagtc aaagtcggtg acatggttcg ctgtgaagca gaaggattca
    14461 tctatccatt tcgtggatat gtagaacacg tctataatca ctcagcgatc attcgcattg
    14521 aaaacacgat ggaatgcgac aagttattag cgaaaagcaa agataattta gcagtggtga
    14581 gattggtgga tatggaggtt ataaacaatg aaatttaaaa tctttgaaga ggacactcgc
    14641 tataaattag aaaaaaaatt aaatgaattt gcaaagaata atgaaattca gcatatatct
    14701 ttaacggctt ctaagtcagg ttatacaact tactatgcag ctgttgtgag ctacgtaatt
    14761 caataaaagg aactcggcaa ataaaaaagc cggatcactc cgactgatgt aataaatccg
    14821 acaagtttat tatatcacat aaaggagcgg tttgacttga tgcaattgtt acgagaggta
    14881 gatttcaaac agacaagatg taatgcgaga gatgtgctga agaactttcg gcgcttggag
    14941 cggatggcag gtcgctcttt gatagatatt aagtcgccga ttattacgga tatgccgaag
    15001 gcaccgaagc acggcaataa ggcagaagac gcgatcattc agatgatgga tatagaagcg
    15061 gagagagacg cgattttagc ggctttgatg gcgcttagtc tgattagccg tcagatactc
    15121 tactacagct tctgtgacgt aaacaagcac tctaattatg aaatagggca attgatacga
    15181 ggatacggag agaagaatgt agagaagctg aaatccatcg cattgatcga atttgctgaa
    15241 gcatacaaaa aaggcgtgtt agttcagtat cgttaatttt gtagggtttt tgtagggata
    15301 gtgtagggtt tttgagcggt ttaacgtgat attatgatag tgtcgaaaga ttagtgatag
    15361 gtctaagaca aaataataat aaaaggaaca tcgttttatt attgtttcac aattaagctt
    15421 cgatagacag cagcggaaat attaagaata aggatgtgaa ttttaacaga attaggcaga
    15481 agtcgcccat cctctatagg tgggtgatga atgccgttcg gtatgagggt taccgttggt
    15541 aactcgaaga ccgacaacta aggcgtattg cctttttatt ttgttccatg ttacaatcag
    15601 tctatacaag aaatgagttg atataacatg gaattagata caaataatca ttcagtattt
    15661 cttctttatt accaccttgt tttagtaacg aaatatcgta gacaagtgat tgatgatgaa
    15721 atatctgatt atgccaaaac tacttttgaa agaatttcag aatcgtatca cattacttta
    15781 gtggagtgga atcatgataa agaccacgtt catattatgt tcaaagctca acctaaaaca
    15841 gaattgacaa aattcatcaa tgcctataaa agtgccagct caagattgat aaaacgtgac
    15901 tttccaagag tcaaacaatt tctttggaaa gaaatgttct ggtctaaaag tttttgtctt
    15961 ctgacaactg gtggcgcacc aattgatgtg attaagaagt atatccaaaa tcaaggaaac
    16021 aatcataaat agaaagtagg tgaactttat ggaacaacta aaagcataca aattcagaat
    16081 ctatccaaca gaggaacaag aaatcttctt tgccaaatct tttggttgtg tccgtaaggt
    16141 ctacaatcta atgcttgatg accgaaaaaa ggcgtatgaa gaagttaaaa atgattcttc
    16201 taaaaaaatg acttttccga caccagctaa atacaagaaa gaatttccgt ttttgaaaga
    16261 aattgatagc cttgctttag ccaatgccca acttaattta gataaagcat acaagaattt
    16321 ttttcgggat aaatccgttg gatttccacg tttcaaaagt aagaaaaatc ctgttcagag
    16381 ttatacaacg aataatcaaa atggtacagt tgctttgatt gatagtaaat tcatcaaagt
    16441 tcctaaattg aagtcattag taagaattaa gcttcataga caaccaaaag ggattatcaa
    16501 atccgctaca atatcacgtc attctagcgg taaatactat atttctttgt tgtgtaaaga
    16561 agaaattatc gaattaccta aaactaattc cacaattggt attgacttag gtattatgga
    16621 ctttgcgatt ctttctgatg gacaaaaaat tgataataat aaattcacgt ccaaaatgga
    16681 aaagaaacta aagcgtgaac aacgcaaatt gtctaggcgt gctttattgg ctaaaaataa
    16741 aggtaccaat ctatttgaag ctaaaaatta tcaaaaacaa aaacgcaaag tagctagatt
    16801 gcatgaaaaa gtaatgaatc aacgtactga ttttctgaat aagttgagta cagaaatgat
    16861 caaaaatcac gatattatct gtattgaaga cttaaacaca aaaggtatgt tgcgaaatca
    16921 taaattagct aaaagtattt ctgatgtttc ttggtcaaag tttgtgacta aattacaata
    16981 caaggctgac tggtatggac gaaaaatcat caaagtagat aaatggtttc cgtctagtca
    17041 gatttgttca gagtgcggac ataaagatgg caagaaatct cttgaaattc gagaatggac
    17101 ttgtcctgtt tgtcatactc atcatgatcg tgatatcaat gctagtatca atattctgac
    17161 cgaaggtcta agactacatt ctatgggatt ggcttagatc caatcatcaa gaaccgtagg
    17221 aactacgggg atagcttggt aaataagaga caccgctgtt agtaaagaaa tacgctatca
    17281 agtatgctct attcccaaga agctcccact tcaagcgtta agaaccgtca ggttta
//
LOCUS       DACEEZ010000058        17140 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20313_2017
            SAMN12639966-rid9879223.denovo.058, whole genome shotgun sequence.
ACCESSION   DACEEZ010000058 DACEEZ010000000
VERSION     DACEEZ010000058.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639966
            Sequence Read Archive: SRR10023158
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 17140)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17140)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741683.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741683.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:44:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,016
            CDSs (total)                      :: 2,962
            Genes (coding)                    :: 2,889
            CDSs (with protein)               :: 2,889
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 36 of 73
            Pseudo Genes (incomplete)         :: 29 of 73
            Pseudo Genes (internal stop)      :: 18 of 73
            Pseudo Genes (multiple problems)  :: 8 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17140
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639966-rid9879223.denovo.058"
                     /isolate="E20313_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            138..1475
                     /locus_tag="IWO89_001953"
     CDS             138..1475
                     /locus_tag="IWO89_001953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate transporter"
                     /protein_id="HAQ0456683.1"
                     /translation="MSDIIIGILLVISFFFMVWYCVKGYNLMVGFAIMATVWMGLALV
                     GNTFSPNPAMEGQGVIDVLTHIYTTGPAEYAKSILVNVFFGAFFGRVLVDSGIAATLI
                     RKVVELGGDKPRITMSLLCIVTAVIFMSMTGIGPVISIAVIVLPILMSLGISVPVALF
                     SFMGSIMAGIFANIVNFKQYQTIYAGFNPAAESYTYNDYFQIGMIGMIVSLVVVLTVA
                     NISMNKKKRYAMAANVPAEGGDAPMISWLAVLLPVLGVVLLDLPIILGFILAGIWALL
                     FTGKLRGGYKEICRQFAKLFTDGAVDVAPMVGFLMTLAMFNNSAAYASPYFSAIFGDW
                     IPQSPLVLAIVFAILTPLGFFRGPMNLVGSGSAILAVVLAVNPTMSPAFLFPLFAITT
                     IAPQHLDITQSWVAWGLGYTKVTSREYMKKSIPTGWIIGAILCLITFLLYGNA"
     gene            1523..3652
                     /locus_tag="IWO89_001954"
     CDS             1523..3652
                     /locus_tag="IWO89_001954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydantoinase/oxoprolinase family protein"
                     /protein_id="HAQ0456684.1"
                     /translation="MKRFVRMGIDVGGTHTKAVAIDNATHEIIGKSSVKTTHDDVRGV
                     AAGVVQSFQNCLLENNISPEDVVFVAHSTTQATNALIEGDVAKVGVIGMAKGGLEGFL
                     AKRQTRLNDIDLGNKKKIEIVNAFLPVKHLNVDRVSETISSLERERAEVLVSSMAFGV
                     DNGEPERVVYEAASVKSIPTTMASDITKLYGLTRRTRTAAINASILPKMLDTATSTED
                     SVREAGVNVSLMIMRGDGGVMEINEMKKRPVLTMLSGPAASVMGSLMYLRASNGVYFE
                     VGGTTTNIGVIKNGRPAIDYSIVGGHPTYISSLDVRVLGVAGGSMVRANQSGIIDVGP
                     RSAHIAGLDYAVFTETEKIKGPKVEFFSPKEGDPADYVKVVMEDGEEVTITNTCAANV
                     LGLVQEEHFSYGNVPSARKAIQALADYCHTTVEDIAEQIMEKSYAKIEPVILELADKY
                     HLEKDQISLVGVGGGAASLITYFSNKMGVKYSIPENAEVISSIGVALAMVRDVVERII
                     PSPSKEDIRSLKNEAMNKAIESGATPESIEVHVEIDPQTSKVTAIATGSTEVKATDLT
                     KEITTEEALELAAEDMRLNKNEVCLLENTPFFYVCGEQNRSKNAGSLRIIDQKGFIKV
                     QRGHASCMKTTAANYMTAVEQLWEDMAVYQTELIARPEFYLCLGARVSDFTATDLEQL
                     QLLMDLEVSTMEPEEEVIVVAGNIKQT"
     gene            3627..4316
                     /locus_tag="IWO89_001955"
     CDS             3627..4316
                     /locus_tag="IWO89_001955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0456685.1"
                     /translation="MPEISNKHKLEELLNELQQIPEEIWGFYQFRRDLFWKKIPLSKQ
                     KILIQQSIDCGIETACSIKKKYPFADVGEICEQMAVPIVSCESEQINERITFATYAED
                     EGIRLMTEPLEKLKCSGLASISKETAQALIIGHELFHHIEASVKGIYTQSEKIVLWRL
                     PFYTHQSNIRALSEIAAMSFSKEMNQSDFSPYVLEAVLLWPYNEIRSQGILEEIKKIE
                     KRCTEYDLDYK"
     gene            complement(4724..5410)
                     /locus_tag="IWO89_001956"
                     /pseudo
     CDS             complement(4724..5410)
                     /locus_tag="IWO89_001956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374310.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
     gene            complement(5546..5740)
                     /locus_tag="IWO89_001957"
     CDS             complement(5546..5740)
                     /locus_tag="IWO89_001957"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0456686.1"
                     /translation="MKRKKQVIPMYVVKVMHGYIDKTGCRTREKNLDNLLIFKDKKES
                     EAFAKRIGGRVKPIQEVRPD"
     gene            complement(5790..6230)
                     /locus_tag="IWO89_001958"
     CDS             complement(5790..6230)
                     /locus_tag="IWO89_001958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase"
                     /protein_id="HAQ0456687.1"
                     /translation="MEKFTGYVREPFYYETDQMGIIHHSNYIRWFEEARVALLVHLGF
                     PYKKIEESGIIIPVLEVNCSYKEMIHYGEKVSIHPTIQKYTGTRLDFSYEIVGTEDKK
                     LKTTGSSKHCFLLSDSQRLVKLSKVNPELDQLFQAYASLDDVNS"
     gene            complement(6234..7079)
                     /locus_tag="IWO89_001959"
     CDS             complement(6234..7079)
                     /locus_tag="IWO89_001959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-hydrate dehydratase"
                     /protein_id="HAQ0456688.1"
                     /translation="MLINEQLVKRVITPRPKISHKGTFGRIVLIGGNEQYGGAIIMSA
                     LAAVHSGAGLTTVVTDEKNRSALHSHLPEAMIVVWTDTDQFLSVIKQADVLLIGPGLG
                     TSSKSLERFKQVLENQSKEQWLVIDGSALSLLAANHLLLPYPEQTVLTPHQMEWQRLS
                     SLPIQEQRDKENKKKQQEIGAVLVLKSHETKIYGHQHFYTNPLGSAAMATGGMGDTLA
                     GMITGFLAQFPQNDQTIAAAIYLHSFIGDQLAKEQYIVLPTQISEALPKWMNYFCKKS
                     MENEA"
     gene            7228..8646
                     /gene="pepV"
                     /locus_tag="IWO89_001960"
     CDS             7228..8646
                     /gene="pepV"
                     /locus_tag="IWO89_001960"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptidase PepV"
                     /protein_id="HAQ0456689.1"
                     /translation="MTIDWQKEVEARKEDLLEDLKNLLRVNSERDDSKVTPDAPFGPG
                     PRDALKHMLAYGERDGFKVKNVDNYAGHIDLGEGDETLGIFGHMDVVPAGDGWDTDPY
                     EPVIKDGKIFARGSSDDKGPSMAAYYAMKIIKELDLKLSKKVRFVVGSDEESGWGDMA
                     YYFEHEEEPDFGFSPDAEFPIINGEKGNVSLALRFKGDNAGDYVLKSFVSGLRENMVP
                     GTATAALQVPSADAAIAMEEAFYQFIEANPVSGTIEADNTYVKIELVGKGAHGASPQS
                     GINAGSFLALFLDNYEFLGSAKQFIHVAAAYVHEDFYGEKLGVAYEDEKMGKLTMNAG
                     LFAFEENGTEEANFINMNFRFPKGVTVDGLQSDIEQTVGQEGATVTRGARVMEPHYVP
                     MDDPLVATLLQVYEDHTGEKGYEQIIGGGTYGRLLKRGVAYGAMFPGYTDTMHQANEF
                     MSLDDLFRATAIYADAIYRLAK"
     gene            complement(8703..9650)
                     /locus_tag="IWO89_001961"
     CDS             complement(8703..9650)
                     /locus_tag="IWO89_001961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HAQ0456690.1"
                     /translation="MEQQEKIAVLIPCYNEEATISTVIADFKRELPEADIYVYDNNST
                     DRTYELAVQGGAIVKKEPRQGKGNVIRQMFFDIDADYYLMVDGDDTYPAEAVHGLLEK
                     LRSGEADMVIGDRLSNGTYFDENKRPFHDFGNNLVRNTITRMYKTKILDVMTGYRGFN
                     RIFVKSFPIMSSGFQIETELTIHALDKKFKFVEIPIDYRDRPEGSESKLNTFSDGFKV
                     IMMIIKMFKDYKPLLFFGIWTIFFFLFGLVTGIPVIREYMLTSFITRIPSAILSTGLM
                     TLALLSLVTGLILDTVVTNAKKEYELNLYHVYHEYHSRK"
     gene            complement(9873..10223)
                     /locus_tag="IWO89_001962"
     CDS             complement(9873..10223)
                     /locus_tag="IWO89_001962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase"
                     /protein_id="HAQ0456691.1"
                     /translation="MYEESELSYFKGGLAVGIGLGLLSGVASTIWYHRKKVLSADQVL
                     ENIKSSFLKEGAIEGSWISFEKEPIRKFAVRSSAYRGGLSRIEDGETVYYEFLADAHT
                     GTVLEISRKKGATD"
     gene            10423..11502
                     /gene="pepA"
                     /locus_tag="IWO89_001963"
     CDS             10423..11502
                     /gene="pepA"
                     /locus_tag="IWO89_001963"
                     /EC_number="3.4.11.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamyl aminopeptidase"
                     /protein_id="HAQ0456692.1"
                     /translation="MEEKTFQRIKELTELQGTSGFEQDVRAYMESHMEPLVDELQLDG
                     LGGIFGLRHHEEADAPRVMVAAHMDEVGFMLTQIQENGLFKVVPLGGWNPYVVSAQRF
                     TLKTSTGKNYPCISSSVPPHLLRGTSGQKSVEVTDILFDAGFESREEAMSYGVLPGDT
                     IVPYAETIKTANGKNIISKSWDNRYGCTMVLEALEALQNETLGHTLIAGANVQEEVGL
                     RGSKASVNKFKPDLFFAVDCSAADDTVTKNGTFGHLGEGTLMRIQDPGLIMLPRLREY
                     LLDIAETNNIPYQYFVSKGGTDAGAAHTQNEGIPSTVIGVVGRYIHTHQTMFSIRDFE
                     AAREMLIQTLKGLDKSTVNTIVYGK"
     gene            11646..11966
                     /locus_tag="IWO89_001964"
     CDS             11646..11966
                     /locus_tag="IWO89_001964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HAQ0456693.1"
                     /translation="MIIPNSLEELAGYVETGKSVFFFTAGWCGDCRFIKPQMPEIEND
                     FSDWQFIEVDRDKYIDVAAEWNIFGIPSFVVIQDGKELGRLVNKDRKTKQEIESFLES
                     VTKG"
     gene            11988..12452
                     /locus_tag="IWO89_001965"
     CDS             11988..12452
                     /locus_tag="IWO89_001965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HAQ0456694.1"
                     /translation="MNQEYKNILVGIDGSEQALAAFKKAVEVARRNNGKVYVANVIDQ
                     QFYNFMGYAPIDQNLVDQQTETAKEMIEECKEYGKSVDYTNIEGIIAYGSVRESMAVK
                     LPKKYEIDLIMVGQSGLNAVERFMTGSVASYVIRHSPCDVLIVSDEEEQPSN"
     gene            12657..13262
                     /locus_tag="IWO89_001966"
     CDS             12657..13262
                     /locus_tag="IWO89_001966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4479 domain-containing protein"
                     /protein_id="HAQ0456695.1"
                     /translation="MIFAYNKAHVGDTLMVIIADDKGTENTVERKWNVARIKDEQGQV
                     VAWNFFHISDHLTIEGNGQVSLTDEELAKLNRLIKEAGFEEQLEADHEPKIVVGYVKT
                     CVPHPDSDHLSITETEVDNGTVLQIVCGAPNIEAGQKVVVAKPGAMMPDGMMIWPGTL
                     RGVDSYGMICSARELHLPNAPAKKGILVLPEDAVVGEAFPK"
     gene            complement(13301..14590)
                     /locus_tag="IWO89_001967"
     CDS             complement(13301..14590)
                     /locus_tag="IWO89_001967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PDZ domain-containing protein"
                     /protein_id="HAQ0456696.1"
                     /translation="MDRKNVTPKMKKSKNNSLWRKLGLGLVGGIVGGLLTAGIFYAAM
                     GTGNNSSTATSGNQNSAGETVVENVKVNVDSDITKAVDKVQDAVVSVINLQSQNQSSG
                     FGGLFGQQEESSSSDDSNLEAYSEGSGVIYKKDGNTAYVVTNNHVVDGQQGLEVLMKD
                     GTKVKAELVGTDAYSDLAVLKINSDKVETVASFGDSSALKVGEPAIAIGSPLGSEYAN
                     SVTSGIISSLNRQVTSTNESNETVNINAIQTDAAINPGNSGGPLVNIEGQVIGINSSK
                     IASTSESTSNVSVEGMGFAIPSNDVVNIINQLEKDGKVTRPALGITMVDLSAVSTQQQ
                     EQILKIPSSVTNGVIVTNVQTATPAEKAGLKQYDVITKIDDKEVSSGVELQSALYKKK
                     VGDSVKITYYEGSKEKTTTVELTIDQSALKQNQNSEN"
     gene            15018..15497
                     /gene="rlmH"
                     /locus_tag="IWO89_001968"
     CDS             15018..15497
                     /gene="rlmH"
                     /locus_tag="IWO89_001968"
                     /EC_number="2.1.1.177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (pseudouridine(1915)-N(3))-methyltransferase RlmH"
                     /protein_id="HAQ0456697.1"
                     /translation="MNIKIISVGKLKEKYLIQGINEYVKRLNAYAKIELIEVPDEKAP
                     ENLSEAQMRQVKEKEGERILAKIKEQEYVYALAIEGKNPTSESFAKQIDQLGIQGKSH
                     LVFVIGGSLGLSEAVMKRSNAQISFGKMTYPHQLMRLILVEQIYRAFRINAGAPYHK"
     gene            15700..16250
                     /locus_tag="IWO89_001969"
                     /pseudo
     CDS             15700..16250
                     /locus_tag="IWO89_001969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299296.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease family protein"
     gene            16321..>17140
                     /locus_tag="IWO89_001970"
     CDS             16321..>17140
                     /locus_tag="IWO89_001970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256-like element ISEfa13 family transposase"
                     /protein_id="HAQ0456698.1"
                     /translation="MNDFTTEIMEILINKGDLDDLFRRHLELAINALLQAELTVFLDY
                     EKYDRAGFNSGNSRNGNYSRSFKTEYGELNLVIPRDRNGDFSQQTLPAYKRTNDSLET
                     TIIQLVQKGITMSEISDLIEKMYGHYYKPQTISNMSKIVSEDVSAFKERTLEAKYSVI
                     FMDATHIPLKRQTVSKEAVYIVIGIRLDGTKEVLGFTIAPTESAYVWKEILQDLKDRG
                     LEEVLLVVTDGLSGIDDSIHSVYPNAQFQQCCVHISRNIAHKVRVSDRQEICSDF"
BASE COUNT         5455 a         3105 c         3466 g         5114 t
ORIGIN      
        1 cataattaag gttttttaat tgaaaactga acatttatgg tcacccttta atgaaagcgc
       61 taactacata agttagaatg agtttgactg atatattagt ttgcttatca gaaaaacaaa
      121 atggacggag gaataacatg agtgatatta ttattggtat tttgttagtt atctcattct
      181 ttttcatggt ctggtactgt gtaaaaggct acaacttgat ggtcggcttt gccatcatgg
      241 ctactgtatg gatgggatta gcgctagtcg gaaacacgtt ttcaccaaac ccggcgatgg
      301 aagggcaagg agtaattgat gtgttgacgc atatctatac gacggggcca gctgagtacg
      361 ccaaatcgat cttggtaaat gtcttctttg gtgccttttt cgggcgtgtt ctagtagata
      421 gcggaatcgc agctaccctg attcgaaagg tagttgaact tggaggggac aagccaagaa
      481 tcacgatgag cttactatgt attgtaaccg ctgttatatt catgtcgatg acaggaatcg
      541 gaccagtgat ttctattgca gttattgtgt tgccaatctt gatgtcatta ggtatttcag
      601 taccggttgc actgttctca tttatgggct cgattatggc tggaatcttt gctaatatcg
      661 tcaatttcaa acaatatcaa acgatttatg ctggtttcaa tccagcggct gaaagctata
      721 cttataatga ctatttccaa attggtatga ttgggatgat tgtaagctta gttgtggtac
      781 taacggtcgc taatatatca atgaacaaaa agaaacgcta tgcaatggca gcaaacgtac
      841 cagctgaagg tggcgacgca ccaatgatct catggttagc cgtattatta ccagttttag
      901 gggtcgttct tttagatctt ccaatcatct taggttttat tctagcaggt atctgggcat
      961 tgctgtttac tggaaaattg cgcggaggtt ataaagaaat ctgtcgtcag tttgcgaaat
     1021 tatttacaga tggcgcagta gatgttgcgc caatggttgg tttcttgatg accttagcga
     1081 tgttcaataa ttcagctgcg tatgcttctc cttatttttc agctattttt ggagattgga
     1141 taccgcaatc accattagtc ttggcaatcg tatttgctat attgacacca ctaggatttt
     1201 tccgcggtcc aatgaatcta gtcggttcag gctcagctat tttagctgtc gtattagctg
     1261 ttaatccaac gatgtcgcca gcctttttgt tcccgttatt tgcaatcaca acgattgctc
     1321 ctcagcattt agatattaca caatcttggg ttgcttgggg attaggttat acaaaagtaa
     1381 cttctcgaga atatatgaag aaatccatcc caactggttg gatcatcgga gcgatcttat
     1441 gcctgatcac gttcttattg tacgggaacg cctagtaaac aaaacgtcga taaaaacgat
     1501 tagatgaatg gaggaaaaga aaatgaaacg ttttgtgcga atgggaattg atgttggcgg
     1561 tacacatacg aaagcagtcg caatcgataa tgcgacgcac gaaatcatcg gaaaatcttc
     1621 agtgaaaaca acccacgatg atgtccgtgg agtagccgca ggtgtcgttc agtcttttca
     1681 aaattgtttg ctggaaaata atatcagtcc agaagatgtt gtgtttgttg cacattctac
     1741 cacacaagcg actaatgcac tgattgaagg agacgtagca aaagtcggcg taatcgggat
     1801 ggcaaaaggt ggattagaag gttttttagc aaaacgacaa actagattga acgatatcga
     1861 tttagggaat aagaaaaaaa tagagattgt caatgcattc ttacctgtca aacacttaaa
     1921 tgtagaccgt gtttcagaaa cgatttcttc tttagaaaga gaaagagcgg aggtcttggt
     1981 ttcttctatg gcatttggag tagataatgg ggaaccagaa cgagttgttt atgaagcagc
     2041 atcggtaaaa tcgatcccta caacgatggc ttctgacatc acaaaattat atggattgac
     2101 aagaagaact cggacagcag caatcaatgc atctatttta ccaaaaatgc tagatacggc
     2161 aacctctaca gaagattccg tacgagaagc aggggtcaat gtttctttga tgattatgcg
     2221 cggagacggc ggagtaatgg aaatcaatga aatgaaaaaa cgacctgttt taaccatgct
     2281 ttccggtcca gcagcatcag tcatgggctc tttgatgtac ttgagagcgt ctaatggtgt
     2341 ttattttgaa gtaggcggaa caacaaccaa tatcggggtc attaaaaatg gacgtccagc
     2401 aatcgattat tcaattgtag gtggccaccc aacatatatc tcttcattag atgttcgggt
     2461 tctaggtgtg gcaggcggtt cgatggtacg ggctaatcag tcaggaatca tcgatgtcgg
     2521 tccacgttct gcacatattg ccggtctaga ttatgcagta tttacggaga cagaaaaaat
     2581 caaaggtcct aaggttgaat ttttctctcc taaagaagga gaccctgctg attacgtcaa
     2641 ggtagtgatg gaagacggag aagaagtgac tattacaaat acatgtgcag ccaatgtcct
     2701 tggattagtg caagaagaac atttttctta tggaaatgtc ccatctgcac gaaaagccat
     2761 ccaagcatta gctgattatt gtcatacgac ggttgaagac atcgcagaac agatcatgga
     2821 aaaatcctat gctaagattg aaccggtgat tttagaattg gcagacaagt atcacttgga
     2881 gaaagatcag atttctcttg taggtgtagg gggcggcgct gcctcattga ttacttactt
     2941 tagtaataaa atgggtgtga aatattcgat tcctgaaaac gccgaagtga tttcttctat
     3001 tggtgtagca cttgcaatgg tccgagatgt tgtagaacgt atcatccctt cgccaagtaa
     3061 agaagatata cgatcgttga aaaatgaagc gatgaataaa gcgattgaat caggcgctac
     3121 accagaatcg atcgaagtcc acgtagaaat cgatccacag acttcaaaag taacggctat
     3181 cgctactggt tctacggaag tgaaagcaac cgatctaacc aaggagatca caacagaaga
     3241 agcactagag cttgctgcag aggatatgcg attaaataaa aatgaagttt gcttattgga
     3301 aaatacacct ttcttttatg tatgcggcga acagaatcgt tcaaaaaatg ctggaagtct
     3361 tcggatcatc gatcaaaaag gatttatcaa ggtccaacgt ggacatgctt catgtatgaa
     3421 aacaactgca gccaattata tgacagctgt cgaacaactt tgggaagata tggcagttta
     3481 tcaaactgaa ttgattgccc gaccagaatt ttatctctgc ttaggagcaa gagtctctga
     3541 ttttacagcg acggatctgg aacaactaca gcttttgatg gatctggaag tatctactat
     3601 ggaaccggag gaagaagtga tcgtcgttgc cggaaatatc aaacaaacat aagttagaag
     3661 agttactaaa cgaattacaa cagatccccg aagaaatttg gggattctac cagtttcggc
     3721 gagatttatt ttggaaaaag attcctttgt ccaagcagaa aattcttata caacaatcaa
     3781 tcgattgtgg tatcgaaacg gcatgtagca tcaaaaaaaa gtaccctttt gccgatgtag
     3841 gagaaatttg tgaacaaatg gctgttccaa tcgtttcctg tgaatcagag caaataaacg
     3901 aacgaatcac ttttgcaact tatgcagaag atgaagggat tcgattgatg acggagccgt
     3961 tagaaaaatt aaaatgttca ggactagctt cgatctctaa agaaacagcg caagctttga
     4021 ttattggaca tgaactattt caccacatcg aagcttctgt taagggaatt tatacgcaaa
     4081 gcgaaaaaat tgtgctgtgg cggttgcctt tttatacaca tcaatcaaac attcgtgcat
     4141 tgagcgagat agcagcgatg agtttttcaa aagagatgaa tcaaagcgat ttttcgcctt
     4201 atgtattaga ggcagtctta ctttggccat ataatgaaat tcgtagccaa gggatcttag
     4261 aagaaataaa aaaaatagaa aaaagatgta cggagtatga tttagattac aaataaagag
     4321 actgtgacaa aagtcgtgtc acagtccttt ttttccgaat aaacggtgtt ctcctgtcaa
     4381 ctcctcggtc acaagtcaaa atattcaaca aacatgagaa gctgtttttt gaaattgttc
     4441 cttgtgactc ggagatgaac ggcaggctca cgacctcttg gaaaacggtg ttcaaaagct
     4501 tgcatctgcg gtaataagtc cagccaagtt aaaaacataa gaagctattt ttacttggct
     4561 gttcttatta cttgatgcag ggcagctttt tcacaacctc tcaataaacg gtgtgcaatc
     4621 agctgaccct cggtattagc tcaaaaaaaa cggcaaaata tgaggaacta ttttgccgtt
     4681 tctttctcta atactcaggt caaaacgctg attccaaaac ctcttatttg atgggtctgt
     4741 cctgaatatc ttcaaaataa tcggggtaat tatgaatcac gtattcttgc tcctcgataa
     4801 tcatatgtaa atgcatcgtg caaagaaatc cgacttcatc aaagttatga tcaatcattg
     4861 attgaagcat tgtctgatgt tcatctaata ctcgacccca gtctaattct gaaatcgtca
     4921 aacgtaacca tctgaaacgg ttcaagtgcg tactataact ttctagccaa tcccaaattt
     4981 ctcgttttcc agcaatttca taacaagttc ggtgaaaatc agtatccaaa tcaaaaaaag
     5041 cttttttatt ttgagcgatt actgcctgat cggtttgttc tagtattttt tctagtttag
     5101 cttgatctta cttggtcatt ttcgcactgc attcttgcag gatcggtttt tccaactttt
     5161 cccgcgtata acgggctaat gaagcagatc ggatatcgat ttttgaaaca taagtgccgg
     5221 actgtggaat ggtttttaca agctcttgat tgcgcagttg gatcagtgct tctcgaatcg
     5281 gtgtacgccc gatagctagt tgttcttcta acactttatc ggagatttta cttccaggtg
     5341 ctaactcgga ataaatgatt tgtttacgta tagattcata agcttgctgt tgcaaattat
     5401 tgataaccat ttaaccttcc tttctatgca gatataatct attataacat aaatcgctta
     5461 caaatcgtgt gggcataaaa acacagtctg caaaaaaagc cctgataggc atgcctatta
     5521 gagcctcgaa aagaaaaata ttgaatcaat ctggtcgtac ctcttggatc ggtttgacac
     5581 gaccgccgat tcttttagca aaagcttcgg attctttttt gtctttgaaa atgagcagat
     5641 tgtctaagtt tttttcccga gtgcgacagc cagttttatc gatgtaaccg tgcattactt
     5701 taacgacata catggggatc acctgctttt ttcttttcat tatacgaact actcgacaca
     5761 atacaagaaa aatgcttaga cggtctttat taagaattta catcatccaa agaagcgtac
     5821 gcttggaata actgatctaa ctccggattg actttgctca gcttcactaa tcgttgcgaa
     5881 tcagaaagta aaaaacagtg tttactactg ccagtagttt ttaatttttt gtcttcagtt
     5941 cccacaattt cgtagctaaa atccaaccgt gtccccgtat acttttggat agttggatgg
     6001 atgctcactt tttctccgta atggatcatt tctttatagc tgcaatttac ttccagaacg
     6061 gggatgatga tcccagattc ttcgattttt ttatagggaa accctagatg taccaataag
     6121 gctacccgag cctcttcaaa ccagcggata taattgctgt gatgaataat ccccatttga
     6181 tctgtttcat aataaaaagg ttctcgcaca taacctgtaa atttctccat gactcatgcc
     6241 tcgttttcca ttgatttttt acaaaaataa ttcatccatt tcggaagtgc ttcgctgatt
     6301 tgtgtcggca aaacaatata ttgttctttt gccagctggt ctccaatgaa actatgaaga
     6361 taaatagctg ctgcaatcgt ttggtcattt tgaggaaact gggctaaaaa tcctgtgatc
     6421 atcccagcta aagtgtcacc catgccgccc gttgccatag cagcagaacc taaaggattg
     6481 gtgtagaagt gctggtgacc ataaattttt gtctcatgac ttttcaaaac gagcacagca
     6541 ccgatttcct gttgcttctt tttgttttct ttatcccgtt gttcttgtat cggtagagaa
     6601 ctcaaccgtt gccactccat ttgatgaggt gtaagtacag tttgttcggg atagggtagc
     6661 aaaagatgat tagcagcaag taaacttaaa gcagagccat caatcaccag ccattgttct
     6721 tttgattggt tttctagtac ttgtttgaaa cgttccaaac tcttagaaga agtacccaat
     6781 ccgggaccaa tcaagagtac atctgcttgc tttattacgg aaagaaactg gtcagtatcc
     6841 gtccaaacta caatcattgc ttctggcaga tgactgtgaa gagcactccg atttttttca
     6901 tcagttacta cagtcgtgag acccgctccg ctgtgtacgg cagctaaagc actcataatg
     6961 attgctccgc catattgttc attgccgcca atcaaaacga tacgtccaaa tgttccttta
     7021 tgtgaaattt ttggacgagg tgtgatgact cttttcacta gttgttcgtt tatcagcatc
     7081 taaaaacctc cttttactca ttatagcgta tatttttttt agagaaaaac agataagaaa
     7141 aggaattgtc agattttttg aactattttt ttctgtaatt tgtggtatca tgaacacgaa
     7201 gacttaaata agtggaggat taactttatg acaattgatt ggcaaaaaga agtagaagca
     7261 cgtaaagaag acttgcttga agacttgaaa aatcttttac gtgtcaacag cgagcgcgat
     7321 gactcaaaag taacaccaga tgcaccattt ggacctggac ctcgcgacgc attgaaacat
     7381 atgttagctt atggcgaacg tgatggattt aaagtaaaaa atgtcgataa ctatgcaggg
     7441 catattgatc ttggcgaagg agacgaaacg ctagggatat ttggacacat ggatgtcgtt
     7501 cctgcaggag atggctggga cacagatcct tatgaaccag taatcaagga tggaaagatc
     7561 tttgcacgcg gatcaagcga tgacaaaggt ccaagtatgg cagcttacta tgcgatgaag
     7621 atcattaaag aacttgactt gaaactatca aaaaaagttc gcttcgtagt cggcagcgat
     7681 gaagaaagcg gctggggcga tatggcttat tatttcgaac atgaagaaga accagatttt
     7741 ggtttctcac cagatgctga attcccaatc atcaatggtg aaaaaggaaa tgtttcttta
     7801 gctttacgct tcaaaggcga caatgcagga gattatgtct tgaaatcttt tgtttccgga
     7861 cttcgcgaaa atatggtgcc aggaacagca actgcagcac ttcaagtacc aagtgcagat
     7921 gcagctatcg cgatggaaga agcattttac caattcatcg aagcaaatcc agtaagcgga
     7981 acgattgaag cggacaatac gtatgtaaaa atcgaattag ttggtaaagg cgcacatggt
     8041 gcaagcccac aatctggtat caatgcaggt tctttcttgg cattgttctt agataactat
     8101 gaattcctag gatctgctaa acaatttatc catgtagcag ctgcttatgt ccatgaagat
     8161 ttttatggag aaaaactagg agtcgcttat gaagatgaaa aaatgggcaa attgacaatg
     8221 aatgctggac tatttgcttt tgaagaaaat ggtacagagg aagcgaactt catcaatatg
     8281 aatttccgtt ttccaaaagg tgtaacagta gacggattgc aaagtgacat cgaacaaaca
     8341 gtggggcaag aaggcgcaac agttactcga ggtgctcgcg taatggaacc acattacgta
     8401 ccaatggatg atccattggt ggctacatta ttgcaagtct atgaagacca cactggagaa
     8461 aaaggctacg aacaaatcat cggtggcgga acttacggac gtttgttgaa acgtggcgtt
     8521 gcttatggag cgatgttccc aggatatact gatacaatgc atcaagcaaa cgaatttatg
     8581 agtcttgatg acttgttcag agcaacagca atctatgcag atgccatcta tcgtttggcg
     8641 aaataattaa ttaaaaagag gatcaagaat tgcaaattat ttttgcagtc ttgatccttt
     8701 ttttactttc tactgtggta ttcatgataa acatgatata gattcaattc atattctttc
     8761 ttagcgttcg tgacgactgt atccaaaatc aaccctgtta ctaatgaaag aagtgctaaa
     8821 gtcatcaatc cagtacttaa aatcgcagaa gggatgcgtg taataaagct tgtcagcata
     8881 tattcgcgaa tcacaggaat ccccgtgacc aatccaaata aaaagaagaa gatggtccaa
     8941 atcccaaaga aaagcaaagg cttataatct ttaaacattt taataatcat catgatcact
     9001 ttgaatccgt cagagaatgt attcaatttt gattcgctgc cttcaggacg atcacgataa
     9061 tcgattggga tttcaacgaa cttgaatttt ttatctaaag catggatcgt caattccgtt
     9121 tcgatttgaa aaccagaact cataatagga aaacttttga caaagatacg attgaatcct
     9181 cgatagccgg tcatgacatc aagaattttt gttttataca ttctagtaat tgtatttcta
     9241 acaaggttat ttccaaaatc gtggaaaggt ctcttattct catcaaagta ggttccattt
     9301 gataggcgat caccaatgac catatctgct tcaccagaac gtaatttctc gagcaaacca
     9361 tgtacagctt ctgcaggata agtatcatcg ccatctacca ttaaataata gtcggcatcg
     9421 atatcaaaga acatttggcg gatcacattt ccttttcctt gacgaggttc tttttttacg
     9481 attgctccac cttgaacggc caattcgtat gttcgatcgg tcgaattatt atcatagaca
     9541 taaatatctg cttctggtaa ttctcgttta aagtctgcaa tgactgttga aattgttgct
     9601 tcctcattgt aacaaggaat cagaacagca attttttctt gttgttccat ttttcactct
     9661 ccttaactat agaaaacaaa agctaaatta aaaatacctc ataatcaaag gaaatgctag
     9721 tttttcccgc taaatatcat ttatttaaaa agaaaatact ggaatatatg tttggaaagc
     9781 tctatctaaa aaaccaccca ctacaaaaga aggacatgat acctctcttt tgaatagtgg
     9841 gtgcgtattc gttctctcac ttttttgacg tattaatctg ttgcaccttt ttttcttgaa
     9901 atttctaata cggttcctgt atgagcatct gctaaaaatt cataataaac agtttcgcca
     9961 tcttctattc gagaaagacc tccccgataa gcactcgacc gaacggcgaa tttcctaata
    10021 ggttcctttt caaaagagat ccaagaacct tcgattgcac cttcttttag aaaagatgac
    10081 ttgatatttt caagtacttg gtctgctgac aatacttttt tgcgatgata ccaaatcgtt
    10141 gaagctaccc cgctcaataa tcctagaccg attccaactg ctaacccgcc tttaaaatag
    10201 cttagttcgc tttcttcata catgtggaca tacctccttc ttctcacttg ttactccttg
    10261 tgcttatttt accacactct catattttct ttgaaataaa taaccgctac cagaaaaaat
    10321 aagctataat aaatacgatc ggtctgcaga aaaaatcata ttttatccgc agatcaatat
    10381 aagtctgaga ggagtttagt ctgaaattta gacgaatagc atatggaaga aaaaacattt
    10441 caacgaatca aagagttaac agaattacaa ggaacaagcg gttttgaaca agatgttcgc
    10501 gcttatatgg aaagtcatat ggaaccgctg gtagatgagt tacagttaga tggtttgggc
    10561 ggcatttttg gtctgcgcca tcatgaagaa gcggatgcac cccgtgtaat ggtcgcagca
    10621 catatggatg aagtcggatt catgctgaca caaatccaag aaaacgggct ttttaaagtc
    10681 gtacctctag gcggctggaa tccttacgtt gtttctgctc agcgttttac acttaaaacc
    10741 tctactggta agaattatcc atgtatttct tcttccgttc ccccacattt attgcgagga
    10801 acaagcggac aaaaatcagt agaagtaacc gatattttgt tcgatgctgg gtttgaatca
    10861 agagaagagg caatgagtta cggtgtcctt ccaggagaca caatcgttcc ttacgctgaa
    10921 acgatcaaaa cagccaacgg aaagaatatt atcagtaaat catgggacaa ccgctatggc
    10981 tgtacaatgg tattagaagc tttagaagcg ttgcaaaatg aaacattagg tcatacattg
    11041 attgcaggcg cgaatgtcca ggaagaagta ggcttgcgtg gttctaaagc ttctgtcaat
    11101 aaattcaagc cagatctgtt ctttgcagta gactgctctg cagcagatga tacagtgacg
    11161 aaaaatggca cattcggaca tttaggagaa ggtacattga tgcgtatcca agatccaggt
    11221 ttgatcatgc ttcctcgttt gcgcgagtac ttgctggata tcgctgaaac aaacaatatc
    11281 ccttatcaat actttgtttc aaaaggcggt acagatgcag gtgctgctca tacacaaaac
    11341 gaaggaatcc caagtacagt gattggcgta gtgggacgtt atatccatac gcatcaaacg
    11401 atgttcagta ttcgcgattt tgaagctgca cgagaaatgt tgattcaaac actgaaaggt
    11461 ttggataaat caacagtaaa tacgatcgta tacggaaaat aatgcgatag tgaaactata
    11521 aaatctggca aaagattgct ttttgccaga ttttttttat aaaaagagga tcaatgtttg
    11581 ttagttcgaa agaaaggtgt ataatttaat tgaaaaatac ataaacagaa agtagaggac
    11641 aaagaatgat tatcccaaat agtttagaag aattagcagg ctacgtagaa actggaaaga
    11701 gtgtattttt ctttactgcc ggctggtgcg gagactgccg ttttatcaaa ccgcaaatgc
    11761 ctgaaattga aaatgacttt tctgattggc aatttattga agtagaccgt gacaagtaca
    11821 tagatgtagc cgctgaatgg aacatttttg gtatcccaag ttttgtcgtg atccaagacg
    11881 gaaaagaatt aggtcgtcta gtgaataaag atcgtaaaac aaaacaggaa atcgaatcgt
    11941 tcctagaaag tgtaacaaaa ggctgacatc tgttaggagg ggtcaagatg aatcaagaat
    12001 ataagaatat tttagtaggt atcgacggta gtgaacaagc acttgctgct ttcaaaaaag
    12061 cggtagaagt ggctcgccgt aataatggga aagtatatgt agcaaacgta atcgatcagc
    12121 aattttataa ctttatgggc tatgcaccta ttgatcaaaa tctagtcgat cagcaaactg
    12181 aaacagccaa ggaaatgatc gaagaatgta aagaatatgg gaaatctgtt gattacacaa
    12241 atatagaagg aattattgca tatggttctg ttcgtgaatc catggcagtc aaattgccaa
    12301 aaaaatatga gatcgatcta atcatggttg ggcagtcagg tctaaatgca gtcgaacgct
    12361 ttatgacagg aagtgtagcc agctacgtga tacgtcattc accttgtgat gtcttgatcg
    12421 tttctgacga agaagaacag ccaagtaatt aagcaagtgg aacttgtacg acctttttaa
    12481 tcaattaact aaaacagtaa agggactgtg acaaaagtct tgtcacagtc ccttgttacg
    12541 aataaatggt gttccagaag aggcttcttc ggaaataaag catgaaaaaa atttcatgca
    12601 gtgaatgcaa ttttttgtta agataaatct cagcaaagga taatggagga aagaacatga
    12661 tttttgctta taacaaagca cacgtcggag acaccttgat ggttattata gccgacgata
    12721 aaggaacaga aaacactgtt gaacgcaaat ggaatgttgc tcggatcaaa gatgaacaag
    12781 gtcaagttgt tgcttggaac tttttccata tctccgatca tctaacaatc gaagggaacg
    12841 gccaagtaag cttaacagat gaggaattag cgaaattgaa tcgattgatc aaagaagcag
    12901 gattcgaaga acagcttgaa gctgatcatg aaccgaagat cgttgtcggg tacgtaaaaa
    12961 catgcgtccc acatcctgat tctgaccatt tatcgattac tgagacagaa gtagacaatg
    13021 gtacagtatt gcaaatcgtt tgcggtgcac ccaatatcga agctggacaa aaagttgtcg
    13081 ttgcaaaacc aggagctatg atgccagatg gaatgatgat ctggccagga actttgcgag
    13141 gggtagatag ctatggaatg atctgttccg ctcgtgaatt gcatttgcca aatgctccag
    13201 caaaaaaagg aatcttagta ttgcctgagg atgccgtagt cggagaagca tttccaaaat
    13261 aaagaggttg tgacaagtct ttgtcacaac ctctttttta ttaattttca ctattttgat
    13321 tttgttttaa tgccgattga tcgatcgtca attcaacagt agttgttttt tctttcgagc
    13381 cttcatagta agtgattttg acagaatctc ccactttttt cttatacaaa gcagattgaa
    13441 gttctacacc agaagaaact tctttgtcat cgatcttagt aatgacgtcg tattgtttca
    13501 atccagcttt ttcagccggt gtagctgttt ggacatttgt cacgatgaca ccgttagtta
    13561 cagatgaagg aatcttcaag atttgttctt gttgctgagt agaaacggca gataagtcga
    13621 ccattgtgat acctagagct ggacgtgtca cttttccgtc tttttccagt tgattgatga
    13681 tattcactac atcgttactt ggaatagcaa atcccattcc ttctacgctt acattagaag
    13741 tagattcaga agtacttgcg atcttacttg agttgatacc gataacttgt ccttcgatat
    13801 taactaaagg accaccagaa tttccagggt tgattgctgc atctgtttgg atcgcattga
    13861 tattgactgt ttcgttcgat tcattcgtac ttgtcacttg tcggttcaag gaagaaatga
    13921 ttcctgaagt gacagaattt gcatattcag atcctagtgg ggaaccaatc gcaatcgcag
    13981 gctcaccaac cttcagagca cttgagtcac caaaagaggc aactgtttcg actttgtctg
    14041 aattgatctt caacaccgct aaatctgaat aagcatctgt tccaactaat tcagctttta
    14101 ccttcgtacc gtctttcatc aagacttcaa gaccttgctg gccatctact acgtgattat
    14161 tcgtgactac ataagcagta ttcccgtctt ttttgtagat cacgccgctt ccttcactat
    14221 aagcttccaa attactatca tctgagctgc tactttcttc ttgctgcccg aatagtccgc
    14281 caaaaccgct tgattggttt tgactttgta agttgataac agaaacgaca gcgtcttgaa
    14341 ctttatctac cgctttggtg atatcagaat ctacattgac tttgacattt tccacaactg
    14401 tttcaccggc agaattctga ttgccgcttg tagcagtaga actattgttg ccagtgccca
    14461 tcgctgcata aaaaatgcca gctgtcagta gtcctccaac gatccctcca acaagcccca
    14521 gacctaactt acgccagagg ctattatttt tacttttttt catctttggt gtcacatttt
    14581 ttctatccat gtgttcctcc tccaattaca ttcatttatc tactgttagt atagtcttat
    14641 ttgttgactt tagtctactt tgagactgtg aactttttat gaaaaacttt aggaaaaatc
    14701 attgttttca gatgaaaata tttgattttc tttttccaca gatactgtgt aaaactacgc
    14761 ggataggatt ggaaaaataa gggataaaac atatccacag cctgtgtata aagtggataa
    14821 attcttataa aacgtatgtt tgcctaatga atgtgatgaa ataaggtaaa ataacactgt
    14881 tttctgtgga taaacctgtg gatgttgtgc ataactgttg gggaaatctt tttaacaaaa
    14941 atagccaaaa taaacatata gacgaaaaaa ttgattataa taaacaaagt gagcatgaag
    15001 accaaaggag agaaaacatg aatatcaaaa ttatctcagt aggaaaatta aaagaaaaat
    15061 acctgatcca aggtatcaat gagtatgtca aacggctgaa tgcctatgca aaaatcgaat
    15121 tgatcgaagt tcctgacgaa aaagccccgg agaatttaag cgaagcgcaa atgagacaag
    15181 taaaagaaaa agaaggggaa cggatcttag cgaaaatcaa agaacaagaa tacgtctatg
    15241 ccttagcaat tgaagggaaa aatcctacaa gtgaatcttt tgctaaacaa atcgaccaat
    15301 taggtatcca aggaaaaagc caccttgttt ttgttatcgg cggctcttta ggactcagcg
    15361 aagccgtcat gaaaagaagc aatgctcaga tctcttttgg aaaaatgacg tatccacatc
    15421 aattgatgcg tctgatcctt gtcgagcaaa tctaccgtgc cttccggatc aatgcggggg
    15481 ctccatacca caaataatga tattttttaa gagaaaaaat tatccctatt ttaaataata
    15541 gcaataggta gtggtaggga gaaatttata aataactttt ttatttgctt tggtagccaa
    15601 gtaagttgca aaagaaaaat agatactgtt aaaaagataa tgtagacata gaaacaaata
    15661 cgtaaaattt tagttagaat aatggcaagg agaaaaaaca tgatagatga aaaaaagtgt
    15721 ttaaaaaatc tattagagga tatggatgat attgaagaaa actatctaaa tggactggga
    15781 aaacaaatat atttcagata cttaagctat ctaaaaatga aataagacat agtaattttt
    15841 tagctttttt gtttgctcca aatgaaaccc acaatttatc tgatgagttt tttaaaatgt
    15901 ttttgaaaag gtatatagat tctaatgatg ataccaaagc tgctatcaat tattttgatg
    15961 ctttacttaa tagctatgaa gatttaattg tttacagaga aaataagaat attgatattt
    16021 tattggtatc ggaaaaaaat aaaatagtgg tgtgcatcga aaataaaatt ctgtcttcgg
    16081 agtctggagg gcaacttaat aaataccaaa tatatataga acgcaattat tctaactata
    16141 aaaaaatttt tgttttttta actccagatg gaaatgaacc cacaaatcct agttggggca
    16201 taattactta caagaatatc gttgaagaga gtgtaaaata ttttgtgtaa atagaaaaaa
    16261 ggaagtccct tctgtagaat agagttacca caacacattc acagaaaaga ggacttccat
    16321 atgaacgatt ttactacaga aattatggaa atactaatca ataaaggtga tttggatgat
    16381 ttgtttcgtc gtcacctaga attggctatt aacgctttat tacaagctga attaacggta
    16441 tttcttgact atgaaaagta tgatagagct ggatttaatt caggtaattc ccgtaatggg
    16501 aattattcgc gctcttttaa aacagagtac ggtgaattga atttagtaat acctagagat
    16561 aggaatggag acttttctca acaaacttta cctgcttata aaagaaccaa tgactcttta
    16621 gaaaccacta ttattcagct agttcaaaaa ggaattacta tgtccgaaat ctctgatcta
    16681 atcgaaaaaa tgtatggtca ttattacaaa ccacaaacta tttcaaatat gagtaaaatt
    16741 gtgtctgaag atgtttcggc atttaaagaa agaactttag aagctaaata ctcagtcatt
    16801 tttatggatg ctactcatat tcccttaaaa agacaaaccg tatcaaaaga agctgtctat
    16861 atagtaattg gtatcaggtt ggatggaact aaagaggttc taggatttac tatagctcca
    16921 actgaatctg cttatgtttg gaaagagatt cttcaagatc taaaagaccg tggtttagaa
    16981 gaggttttgt tagtcgtaac agatggttta agtggtattg acgatagtat tcatagtgtt
    17041 tatccaaatg ctcaatttca acaatgttgt gtccatatat ctagaaatat cgctcataag
    17101 gttcgtgtta gtgatcgaca agaaatctgt agcgatttca
//
LOCUS       DACEEZ010000059        17022 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20313_2017
            SAMN12639966-rid9879223.denovo.059, whole genome shotgun sequence.
ACCESSION   DACEEZ010000059 DACEEZ010000000
VERSION     DACEEZ010000059.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639966
            Sequence Read Archive: SRR10023158
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 17022)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17022)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741683.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741683.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:44:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,016
            CDSs (total)                      :: 2,962
            Genes (coding)                    :: 2,889
            CDSs (with protein)               :: 2,889
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 36 of 73
            Pseudo Genes (incomplete)         :: 29 of 73
            Pseudo Genes (internal stop)      :: 18 of 73
            Pseudo Genes (multiple problems)  :: 8 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17022
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639966-rid9879223.denovo.059"
                     /isolate="E20313_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            226..894
                     /locus_tag="IWO89_001971"
     CDS             226..894
                     /locus_tag="IWO89_001971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF421 domain-containing protein"
                     /protein_id="HAQ0456699.1"
                     /translation="MIFSITMKLVFGLIGLLLVVRLLGKKSMSEITPFDLVYTLVLGG
                     ILEESTYDDNVHVGHVLFAIALWAVMIYGIERIVQKNEKVNRWVKGEPSVLIKDGVIN
                     MTELTNNHIEMEQLRAILRQQECFSLENAKHVILENAGQMSVLKKSDEDKALSILLVD
                     EGQIQHKVLQSNQLTEAWLMENLKKEGYADVKQLIYVEWSEEKGLYILNKEDVMHESY
                     RIDG"
     gene            complement(1094..1480)
                     /locus_tag="IWO89_001972"
     CDS             complement(1094..1480)
                     /locus_tag="IWO89_001972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0456700.1"
                     /translation="MRRTAKKFIDYNEYRDRPFGLKWGTAFAMEELVNGIKKNEEEAR
                     KQPLPKQQMTLQEIDSILTEAFLSRKAVIIQLNLKDEFGRLLDEVEGHFIGEAYEDYF
                     MIDHQPILWEDVRYIELKETKKWFVT"
     gene            complement(1452..2834)
                     /locus_tag="IWO89_001973"
     CDS             complement(1452..2834)
                     /locus_tag="IWO89_001973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Y-family DNA polymerase"
                     /protein_id="HAQ0456701.1"
                     /translation="MWANQNPVFVYEKEPSRDILCIDCKSFYASVECVEKDLDPLTTK
                     LVVMSYPSDSTETRGSGLILASSPAAKKAYGITNISRARDLPFPYPSDLYIVAPRMAY
                     YMEKNREINSIYKKYADEANHHVYSVDESFLDVTNSMALFGKTNAYDMAKLIHKDVFE
                     QTGIYTTIGIGDNPLLAKLALDNGSKDGKQMFAEWRYEDVPEKLWKISDITDFWGIGK
                     RTAKRLKQMGIHSVYDLAHADYYHLKSKLGVIGTQLYAHAWGIDRSFLGQEYTPKSKS
                     IGNSQILDRDYTRREEIEIVIKEMADQVGTRLRRSAAKTQVVSLWVGYSLGYLDPNGK
                     TGFHQQMKIEATNASRDLADALLTIFHQHYHYQDIRRVGVNCSDLVYATGLQLNLFED
                     PEKQINEAKVDFVVDTIRKKYGFQSIIHAHSLLQGGRAIARSSLVGGHAGGMSGLEGG
                     KDETDSKKIH"
     gene            complement(2990..4177)
                     /locus_tag="IWO89_001974"
     CDS             complement(2990..4177)
                     /locus_tag="IWO89_001974"
                     /EC_number="2.7.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactokinase"
                     /protein_id="HAQ0456702.1"
                     /translation="MEIKRSLNTKFAETFGPKKTTQYFSPGRINLIGEHTDYNGGHVF
                     PASITYGTQGVAAPRDDNKILVYSTNFDDIGVISFTLDELEYDKKANWANYVKGMVLK
                     LKEAGHSIDHGFELLVEGTIPNGAGLSSSASLELLVGVVLEDLFDLKTDRLELVQTGK
                     KVENEFIGVNSGIMDQFAIGFGELDHAILLDTNTLKYEMVPVKLDGYAIVIMNTNKRR
                     ELADSKYNERRSECEEALARLQKTLPISSLGDLDEEEFESTIDQIGDDTLIRRARHAV
                     SENQRTLKAKAELEAGNLEAFGQLLNDSHHSLRYDYEVTGIELDTLVDAAQKQEGVLG
                     ARMTGAGFGGCAIALVKEENIPAFKNNVYDEYMKVVGYAPEFYVAHIGNGTTKIDPEA
                     VHV"
     gene            complement(4184..5215)
                     /locus_tag="IWO89_001975"
     CDS             complement(4184..5215)
                     /locus_tag="IWO89_001975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose mutarotase"
                     /protein_id="HAQ0456703.1"
                     /translation="MKITEKDFGQGYHLITLENKNKLALSISDLGARIVSLKSNDREL
                     VLGFDTAEEYIEKDPYIGASIGRTAGRIENGRFSLNGKTYQLATDPKTGHNLHGGAPG
                     FELKKWSYVILNGENEASVIFTTTSPDGEHGFPGTMDVEIRYTLTKDNIWRVTSRGTS
                     DQDTLFNPTNHVYFNLTGDASQSIDQHELWLNSEAYAPLRTDSIPIGVKENAAGSAFD
                     FQIPKKLASVFASDLDQKNLVDGIDHPFFLKETGLGKEAARLTSPDKRIQVGIATDAS
                     SVVIFTANFGTETPEMRNRKLAHHGGITFETQTAPGAERFSAFGSIHLKAGSVFETVT
                     EFKIKTRKE"
     gene            complement(5231..6685)
                     /locus_tag="IWO89_001976"
     CDS             complement(5231..6685)
                     /locus_tag="IWO89_001976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAQ0456704.1"
                     /translation="MKSKMIQRVSYALGAFGHDTFYVTLSTYFMVFVTSQMFAGTDKA
                     TNAKMIATVTSLVVGIRLVEIIFDPLIGGIIDNTRTKYGKFKPWLVIGGVVSSVLLVA
                     IFTNFFGLATHNTTLFIILFAIVFILLDSFYSFKDIAFWSMIPALSTETKEREKFATF
                     ARFGSSLGANGTTLVVVPIVTFFTYLVTHKHIEGASGWFWFGVIAAVISGGTAIITAL
                     GTKEVDTAIRQQNEKAGIKDVFKAVAQNDQLMWLALAYIAYAIANVATTAVLFYFFKY
                     VLGQPTEFWIVGLVAALLGLVAVPLFPMLTRVISRRYVYLGGIAMMISSYFFFTIAGS
                     SLPIVIIGLVLFYFPQQLIFLSALMTITDSVEYGQWKNGIRNEAVTLSIRPLLDKIAG
                     ALSNGIVGFVAIASGMTGNATAADITAHGVTTFKAYAFYAPAALMIFSAIIFAWKITL
                     TEKKHAQIVEELENKLAPADTMEDELHDAVVPSH"
     gene            6860..7765
                     /locus_tag="IWO89_001977"
     CDS             6860..7765
                     /locus_tag="IWO89_001977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HAQ0456705.1"
                     /translation="MEQSIFLKKEHQQVEKELYFDFCGFSKTMPFHSFGPAIRNDYIL
                     HVVMEGKGSYHVKNQQFQLKKGDLFLIRPGDSTFYLSDGEDPWMYCWLSFGGSAAEEI
                     IRQSLFREDQYTMVSSDIQKYIDIILECMKDTEETLENELQLTALTYRFLSVLLKDGG
                     KVSGGVQKVHSSLAVETVKYIEAHYAEKLSVEEIAQHLAVNRSHLSRVFKNHMGTSIK
                     EYLIGVRINRGAFLLSLTDESVESIAYQVGFNSLVVFSRMFKKSTGETATNYRKRMKN
                     ENFKNIALDTLKKQLEKQTAVSWTT"
     gene            7856..8533
                     /locus_tag="IWO89_001978"
     CDS             7856..8533
                     /locus_tag="IWO89_001978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HAQ0456706.1"
                     /translation="MRTLIIISHPTIEESLNQQFFKEASAGLPATWHHLESSYPDGKI
                     DIETELQLLAQHDRIIFQFPFYWYSSPAHLKIWQDNVLERAAHVLKGKELGIVLTTGV
                     AEKEYQAGGKEEYTISEFLRPYQRIANKFHMTYLPPFVLAQFMYLSQEKRWEKLIAYQ
                     QYLSLEGKPSLTQRIDWFIQRVQENQKMQEEESEKQAYIVEALTEAKEQIEDLTFTLQ
                     EMKGTSL"
     gene            8814..9710
                     /locus_tag="IWO89_001979"
     CDS             8814..9710
                     /locus_tag="IWO89_001979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HAQ0456707.1"
                     /translation="MYDENEWGENVRMTRRRQQKQAARKRMIMIGAAVAVFLIIAGGV
                     AGLYFHGKNSSEAKSTTITETTTKKTETKQTSESSTKTKETQTSEEKTANGLPEQLQA
                     IVNDYSKKIEEKTPALIEEYQTEIQGNQEGIAGLSAVANQKARELQAISDEGIRKLRA
                     AYQAAENKDGVDLDTLINQLSANYTNHVAKISDIYLQTSASLQAESTSTQDTTSSDSE
                     TAESVEASEMQARTTQDTTDSSETNTNSATSQAATTVVLEGEGPNQIAERTGVPVETI
                     LSLNGMSMENYFLTPGETLKLN"
     gene            complement(9765..11135)
                     /locus_tag="IWO89_001980"
     CDS             complement(9765..11135)
                     /locus_tag="IWO89_001980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAQ0456708.1"
                     /translation="MEEEKLTRSLSARHIQMIALGGTIGVGLFMGASSTIRWTGPSVM
                     GAYAIAGFFLYLIMRALGEMLYVDPSTGSFANYASEYIHPVAGYLTAWSNIFQYIVVG
                     ISEVIAVGSYMNYWWPDLPAIIPGIIVVLFLMLANLISVKAFGELEFWFSIIKVITII
                     LMIIAGLGVILFGFGNHGQAVGISNLWKNGGFFTGGVKGFFFALSIVVASYQGIELIG
                     MTAGEAENPKKTIIEAVQSTIGRILIFYIGAIFIIVSIYPWNQLSEIGSPFVQTFSKV
                     GITFAAGLINFVVITAALSGCNSGIFSASRMVYTLAINGKMSKKFLKLSRHGAPFYPV
                     VAISGGILIGLILNYLLPMFINQSENIFVFVYSSSILPGMVPWVVILISEIRFKKKHA
                     DKMHDHPFKMPLSPYTNYVTLAFLAIVLIFMFINPETRISLIIGLVFLLYMTIHYFVR
                     EKRTGK"
     gene            11373..12119
                     /locus_tag="IWO89_001981"
     CDS             11373..12119
                     /locus_tag="IWO89_001981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HAQ0456709.1"
                     /translation="MERTAIVIQDLSAAYQGKTVLKTINLTINPQKITGIIGPNGAGK
                     STFMKSLLELIPATTGKVTFSGQPVNSVRKKIAYVEQRSELDLSFPIDVLGVVLLGTY
                     PSLRIGQRPGKKEKERARQALKKVGMEEYAKRQISELSGGQLQRVFIARALAQGAEWI
                     FLDEPFVGIDALSERKIFDILQELKNSGKTILIVHHDLHKVEEYFDEVILLNKQLIAS
                     GPVQEVFTSKNLQLAYGEIIRHLVKGGEKK"
     gene            12116..12955
                     /locus_tag="IWO89_001982"
     CDS             12116..12955
                     /locus_tag="IWO89_001982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HAQ0456710.1"
                     /translation="MIQSFIDGLMNYQFLQYALVTSMLVGLASGVIGSFIILRGMSLM
                     GDAISHAVLPGVAISYMFGFSYIFGATAFGMLTAAVIGFVTQRSRLKNDTAIGIVFSS
                     FFALGIILISYAQSATDLYHILFGNVLAVRESDLLLTALVSGIVLIFVFFFYKELKIT
                     SFDPTMAKAYGLNTSLIHYLLMFFLTLVAVVSLQTVGTILVIAMLITPAATAYLLTNH
                     LLKMIITAAGIGMLSAVVGVFFSYSYNWPSGATIVLACTAFFILAFLFSPTKGILFQK
                     GSR"
     gene            12956..13906
                     /locus_tag="IWO89_001983"
     CDS             12956..13906
                     /locus_tag="IWO89_001983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter substrate-binding protein"
                     /protein_id="HAQ0456711.1"
                     /translation="MKTKKSLFLILAVSFLVLAGCGKQASDQADEGSKEKLSVVATNS
                     ILADMAKEVGTDQIDIHSIVPVGTDPHEYEVLPEDIKKASDADVILYNGLNLETGNSW
                     FDNLMETAKKEEGKDYFAVSKNVEPLYLTSGEEHTKADPHAWLDLSNGIKYVEEIARI
                     FSEKDAENATLYKKNAEAYVEKLKELDTQAKESFASIEENKKLLVTSEGAFKYFSRAY
                     DLPAAYIWEINTESQGTPDQMKAIIDQIRAKEVPVLFVETSVDSRSMERVAKETGLKI
                     YDKLFTDSIAKEGEQGDSYYQMMKWNIETIHEGLSQTKES"
     gene            14047..14799
                     /locus_tag="IWO89_001984"
     CDS             14047..14799
                     /locus_tag="IWO89_001984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0456712.1"
                     /translation="MNFTRKDKYTIKEEVFRLISQQENTDKDQDLQFLEAELAKLFIA
                     NQNETTEPDEELYIGKWLFDEFQPLDENTGIGYFVFLSPVGFTPGVLVKQNDEITVKA
                     WPALFSVIKAEGVIKKIQSAYYKSQFIANSKLMNMFYNRLVEIARPGKIPQTFTYTQQ
                     KNLYEEVLYSRDLSEFSPWEEVRLHELLSTKPVSLNFFNALDNKRKLISTNYWEVFAA
                     RKKWMEEKKIADQQIQFVQLKKEKLYYGLYSR"
     gene            14818..15969
                     /gene="queG"
                     /locus_tag="IWO89_001985"
     CDS             14818..15969
                     /gene="queG"
                     /locus_tag="IWO89_001985"
                     /EC_number="1.17.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA epoxyqueuosine(34) reductase QueG"
                     /protein_id="HAQ0456713.1"
                     /translation="MTLKEEIIQESKRLGIDKIGFTTAEPFDSLKESLEEQKAAGHTS
                     GFEHPNIDERLYPEMTFDQPKSIISIALAYPTRIHEEVPRDEKRGQFARASWGIDYHD
                     ILRDRLNRLIEFIQTRAEKWQKEEEWRLAPQVDTGELIDVAVAQRAGLGFIGRNGLLI
                     TEEFGSFVYLGEIVTNIVFEPDKPGEFGCGDCMRCVTACPTKALLGDGRMNAQRCLSY
                     QTQTKGMMPEEYRKKMRNVIYGCDICQLVCPYNQGKDFHFHEEMEPKVDEVYPKLKPM
                     LSMSNKDFKQQFGHLAGSWRGKKPLQRNALIALANLGDRTALPEISECLTDVRPVIRG
                     TAAWAIGRLGTKEPEKWLDVLDEILAKETEEVVINELEHAQKLLRRKLK"
     gene            16353..16961
                     /locus_tag="IWO89_001986"
     CDS             16353..16961
                     /locus_tag="IWO89_001986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HAQ0456714.1"
                     /translation="MNSFKKIVLGTTFAAGATAMFVGTTNAHADEVYTVKSGDSLSKI
                     SQKFAGDNSMIDAIAEKNSIANINRIYVGEQLTIPTSNDSSATTENKTTENTASTTET
                     ATQEHTYVAPVETVEVAPAAPAAATAPTSSSAKEWIAQKESGGSYTATNGRYIGRYQL
                     DASYLNGDYSAANQERVAEQYVTSRYGSWDAAKTFWLANGWY"
BASE COUNT         5560 a         3172 c         3392 g         4898 t
ORIGIN      
        1 cgtccaattt cgtaaaacaa ccgtaacggt tgtgtaaaaa tatcatttta gagcataaaa
       61 aaaaggagtg gatttctcca ctccaaaaaa gtctaacttt tgggggtcac tacagtgtca
      121 cagtcctttt tttagtaaaa ggatacattt cttccttgta tattccatgc gtgttcgtga
      181 gaaagcatat ataatttaat taacagatag ttagggggag aaaaaatgat tttttcaatt
      241 acaatgaaac tggtgttcgg gttgatcggt ttattattag tggtacgttt gcttgggaag
      301 aaatccatgt cagaaatcac accgtttgat ttagtctata cgcttgtttt aggtgggatc
      361 ttagaagaat caacctatga tgacaatgtc catgtagggc atgtcctatt tgccattgcg
      421 ctttgggcag taatgattta tggtatcgaa cgtatcgtcc aaaaaaatga aaaggtcaat
      481 cgttgggtca aaggcgagcc atctgttcta atcaaagacg gcgtcatcaa catgacagag
      541 ttgacaaata accatatcga gatggagcag ttgcgagcaa tcttaagaca gcaagaatgc
      601 ttttctcttg aaaatgccaa acatgttatc ctagagaatg ctggtcaaat gagtgtattg
      661 aaaaaatcag atgaagacaa agctttatcg atcctgcttg tagatgaagg gcaaattcag
      721 cataaagtgc ttcagtccaa tcaattgact gaagcatggc ttatggagaa cttgaagaaa
      781 gaaggctatg cagatgtaaa acagctgatt tatgttgaat ggtcagaaga aaaagggctc
      841 tacatactaa ataaagaaga cgtcatgcat gaatcttacc gaatagatgg atagatagac
      901 agaggttgtg aaatcagcgc cttggcttgt tactcaaaac taagcgactt tttcacaacc
      961 tctgatatct aatatacgtt gttccagaag ttgcttcttc gaaaaataag ccaaaattcc
     1021 acaaaaattt aagaagcaat tttcgaaaaa tcctttctta ttttttgaag ttgatcactt
     1081 ctgtcacaac ctctcatgtc acaaaccatt tttttgtttc ctttaactca atataccgta
     1141 cgtcttccca caatatcggc tgatggtcaa tcataaagta atcttcatag gcttcaccaa
     1201 tgaagtgccc ttcgacctca tccaagagtc ttccaaattc atcttttaaa ttgagctgaa
     1261 tgattactgc tttacgagat aaaaaggctt ctgtcagtat actatcgatt tcttgcaatg
     1321 tcatttgttg tttaggaaga ggctgtttgc gtgcttcttc ttcatttttt ttgataccgt
     1381 tgactagttc ttccatcgca aaagcagtcc cccattttaa tccaaatggc cgatctcgat
     1441 actcgttata gtcaatgaat ttttttgctg tccgtctcat cttttccacc ttctaatcca
     1501 ctcataccac ctgcatgccc gccaacgaga gaactacgtg cgatggcgcg tcctccttgc
     1561 aagagactat gcgcatggat gattgattga aagccgtatt tttttctaat cgtatcgaca
     1621 acaaaatcaa ctttcgcttc atttatctgc ttttcaggat cttcaaaaag gttaagctgt
     1681 aaacctgtcg cgtatacgag gtcggaacag ttcacgccta cacgccgaat atcctggtag
     1741 tggtaatgct ggtggaaaat ggtcaataaa gcatctgcta gatctctgct agcatttgtc
     1801 gcttcgattt tcatttgctg gtgaaacccg gtcttgccat ttggatcaag atagcctaaa
     1861 gagtatccta cccataagct gactacttgt gtttttgctg cagatcgtct caagcgagtg
     1921 ccgacttggt cggccatttc tttgataacg atctcgattt cttctcgtct tgtataatct
     1981 ctatctagga tttgactatt tccgatactt tttgattttg gtgtatattc ttgtcccaaa
     2041 aaacttcgat cgatgcccca agcatgggca taaagctgag taccgatgac ccctagcttg
     2101 cttttcaagt gataataatc cgcgtgagct aaatcataaa cagaatgtat acccatttgt
     2161 ttcagacgtt ttgctgttcg tttgccgatt ccccaaaagt cagtgatatc tgagattttc
     2221 cataactttt ctggtacatc ttcataacgc cattcagcaa acatttgctt tccgtctttt
     2281 gagccattgt ctaaggctag tttagcaagt agcgggttat cgccaattcc tatagtcgtg
     2341 tagattccgg tctgctcaaa cacatcttta tggatcagtt tagccatgtc gtacgcattt
     2401 gttttaccaa agagtgccat gctatttgtt acatcaagga agctttcatc gacggaatag
     2461 acatgatggt tcgcttcgtc tgcatatttt ttatagatag aattgatttc tctgttcttt
     2521 tccatataat aggccatcct tggggcaaca atatataaat cacttggata ggggaaggga
     2581 agatctctgg cacggctgat attggtgatg ccgtaagctt tttttgctgc aggactggaa
     2641 gccaagatta atccgctacc ccgtgtttct gtcgaatcgg acggatagga catgactact
     2701 aatttagttg taaggggatc aagatccttt tcaacgcact ctacggaggc gtaaaaagat
     2761 ttgcagtcaa tacataaaat atctcttgaa ggttcttttt cataaacaaa taccggattt
     2821 tgattcgccc acataacaat cacctcttca acacaattag aacacacgtt ctctttccgt
     2881 gtcaataaaa aacagcaatt ttcaaatacg agcaaaaggc tcacatttgg aaattgctga
     2941 gaggttattt ttatctattt gtttttagct gagatcgttg atttttttat caaacatgaa
     3001 cagcttctgg atcgatcttt gttgtcccat ttccaatgtg tgcaacataa aattccggag
     3061 catagccgac aactttcatg tattcatcat agacattgtt tttgaaggca ggtatatttt
     3121 cttctttgac aagagcaatc gcacagccac caaatccagc tcctgtcatt cgtgcaccta
     3181 agacaccttc ttgtttttga gcagcatcta ctagtgtatc taattcgatc ccagttactt
     3241 cataatcata gcgaagagag tggtgagaat catttaacag ttgaccaaat gcttctaagt
     3301 tcccagcttc taattcagct tttgccttca aagtccgctg attttcagaa acggcatggc
     3361 gagctctgcg tatcaatgta tcatcgccga tttggtcaat agttgattca aattcttctt
     3421 catctaagtc tcctaaagaa gagataggca aagttttttg caaacgtgcc aaggcttctt
     3481 cgcattcgct acggcgttca ttgtattttg aatctgccaa ttcccgccgc ttattcgtat
     3541 tcatgatgac gattgcataa ccatctagtt tcactggaac catttcgtat ttcagcgtat
     3601 ttgtatctaa taagattgca tgatctagct caccgaagcc gatggcaaac tgatccatga
     3661 tacctgaatt gacaccgatg aattcatttt ctactttttt acctgtttgg actagttcca
     3721 agcgatctgt ttttaaatca aacagatctt ctaatacaac acctactaat aattctaatg
     3781 aagcgctact ggaaagtcct gcaccattag ggattgttcc ttccactaac aactcaaacc
     3841 catgatcgat tgagtgtcca gcttctttta atttaaggac cattcctttt acgtaatttg
     3901 cccagtttgc ttttttatca tattctagct catctaaagt aaaactaatg acaccgatat
     3961 cgtcaaagtt tgtggaataa actaatattt tattatcgtc tcgaggtgct gctacacctt
     4021 gcgttccata agtgatggaa gcggggaaga catggcctcc attgtaatct gtgtgttcac
     4081 caatcaaatt gatgcgtcct ggtgagaagt attgtgtagt ttttttaggt ccgaatgttt
     4141 cggcaaattt tgtgtttaag cttcttttga tttccatgat gagttattcc tttcttgttt
     4201 tgattttgaa ttctgtcact gtttcaaata ctgatccagc ttttaaatga atcgaaccaa
     4261 atgctgagaa gcgctctgca cctggagctg tttgagtctc aaatgtgatg ccaccgtgat
     4321 gtgccagttt tcggttgcgc atttctggtg tttctgtgcc gaaatttgca gtgaaaataa
     4381 ccacactaga agcatctgta gcaataccta cttggattct tttatcgggg ctagtcaagc
     4441 gagcggcttc tttacccaag ccggtttctt ttaagaaaaa tggatgatcg attccgtcaa
     4501 caagattttt ttgatccaaa tcactagcaa aaacggacgc taatttttta ggtatttgaa
     4561 aatcgaaagc acttcctgca gcattttctt ttacccctat aggaatacta tctgttcgca
     4621 aaggagcata agcttcgcta ttgagccaaa gctcatgttg atcgattgac tgagaagcat
     4681 ctcccgtcaa gttgaagtaa acatgatttg tcggattgaa taaagtatct tggtcactgg
     4741 tgccgcgaga ggtcactcgc cagatattat cttttgtcaa agtatagcgg atttccacat
     4801 ccattgtacc tggaaaacca tgttctccgt cgggacttgt agtagtaaaa atgacagaag
     4861 cttcattttc gccattcaaa atgacataag accatttttt taattcaaat ccaggagcac
     4921 caccatgcag attatgtcca gtttttggat ctgtggctaa ttggtaagtt tttccattga
     4981 gagaaaagcg gccattttcg attctccctg cggtgcgacc gatagaagca ccgatatacg
     5041 gatctttttc gatatattct tcagcggtgt caaatcccaa aactaattct cgatcatttg
     5101 attttaaact gacgattcta gcacctaaat cagagataga aagtgctagc ttgtttttgt
     5161 tctccaaggt tatcaagtga taaccttggc caaaatcttt ttctgtaatc ttcattttca
     5221 agatcctttc ttaatgggaa ggaactactg catcgtgaag ttcatcttcc attgtatcag
     5281 ctggagccaa tttgttttcc aattcctcaa cgatttgtgc atgttttttc tctgtcaatg
     5341 taatcttcca agcaaaaata atagcggaga aaatcatcaa agcagcagga gcatagaatg
     5401 cataagcttt gaaagtcgtt acgccatgag cagtgatatc agcagcagta gcattaccag
     5461 tcatcccaga agcgatagcg acaaacccta cgatcccgtt agacaaagca ccagcgattt
     5521 tatctagtaa agggcgaatt gatagagtaa cagcttcgtt acggatacca tttttccatt
     5581 gaccatattc aacagagtct gtgatcgtca tcaaagcgga taagaaaatc aattgttgag
     5641 ggaagtagaa caatactagc ccgatgataa cgattggcaa acttgaacct gcgatcgtaa
     5701 agaaaaagta agaagaaatc atcattgcga tccctccaag gtaaacgtag cgacgagaga
     5761 tgacgcgtgt aagcattggg aatagtggaa cagctactaa accaagtaag gcagcaacta
     5821 gaccaacgat ccaaaattca gttggttggc caagtacata tttgaagaag tagaataata
     5881 cggcagttgt tgctacattg gcaatcgcat aagcgatata agcaagggca agccacatca
     5941 actgatcatt ttgagcaact gctttgaaca catctttgat cccagctttt tcattttgct
     6001 gacggatcgc agtatcgact tcttttgtgc ccaaggcagt gataatcgca gtaccgcctg
     6061 aaataacagc agcaatcaca ccgaaccaga accagcctga agcgccttca atatgtttat
     6121 gagtaacaag ataggtaaag aaagtaacga tagggacaac gactagagta gtaccatttg
     6181 cacctagaga agagccgaaa cgagcaaacg tagcaaattt ttcacgttcc tttgtttccg
     6241 tactcaaagc tggaatcatt gaccaaaaag cgatatcctt aaatgaataa aaactatcca
     6301 ataagatgaa aacgattgca aataatatga taaataaagt cgtattatga gtagctaagc
     6361 caaagaaatt ggtaaagatt gctaccaaga gtacggaact aacaacgccg ccgataacta
     6421 accatggttt gaatttaccg tattttgtac gagtattatc gatgatgcca ccaatcaaag
     6481 gatcaaaaat aatttcaact aagcggatac ctacaactaa gcttgtaact gtagcaatca
     6541 tttttgcatt cgttgcctta tctgttcctg cgaacatctg gcttgtcacg aataccataa
     6601 aataagtgct taatgtaaca tagaatgtgt cgtgtccaaa agcgcctaaa gcgtaggaca
     6661 cacgttggat cattttgctt ttcataatct gtattcctct cctgttcaat atataatact
     6721 aagtaaaccg gttacaacga ataacaaaga tcttttaatg acttaaactt aacaagaaat
     6781 cgcttccttt ttaatatatt tctgtgactt ttatttaact ttttgttgca ttatactaaa
     6841 ggagtaagga gggatagacg tggagcaatc gattttttta aaaaaggaac atcaacaagt
     6901 agaaaaagaa ttgtattttg atttttgtgg tttctctaaa acgatgccgt ttcattcttt
     6961 tggtcctgct attcgcaatg attacatcct gcatgtcgtg atggaaggca aaggatctta
     7021 tcatgtgaaa aatcaacaat tccaattgaa aaaaggtgat ctgttcttga ttcgtccagg
     7081 agactcgacg ttttacttat cagatggaga agatccgtgg atgtattgct ggctttcatt
     7141 tggtgggagt gctgcagaag aaatcattcg gcagtcgctt ttcagagaag accaatacac
     7201 gatggtctct tctgacatac aaaaatatat cgacattatt ttagaatgca tgaaagatac
     7261 ggaagaaacc ttggaaaatg aattacagtt aacagcatta acctaccgtt ttttatcagt
     7321 ccttttaaaa gatggcggaa aggtcagcgg tggtgtacaa aaggttcatt cctctttagc
     7381 tgttgaaaca gtcaaataca tcgaggcaca ttatgctgaa aaattgtctg ttgaggaaat
     7441 cgcccagcat ttagcggtca atcggagcca tttgtcgcga gtattcaaaa atcatatggg
     7501 taccagtatc aaggaatatt tgatcggggt acggatcaat cgaggggcgt ttcttctttc
     7561 tctgacggat gaatccgtgg aaagtattgc ctatcaagta gggttcaata gtctagtcgt
     7621 atttagccga atgttcaaaa agagtaccgg agaaacggct acgaactatc gtaaacgtat
     7681 gaaaaacgaa aattttaaga atattgcttt ggataccttg aaaaaacagt tggaaaaaca
     7741 aaccgcagtt tcttggacaa cctgattagt ctggttaaaa tgacaagcat cggcaaaaga
     7801 aacttgtggc ggttatgcta cactgtaaag cgaagactaa aaaggaggaa aagggatgcg
     7861 tacacttatc atcattagcc atccaacgat cgaagaatct ttaaatcagc aatttttcaa
     7921 agaagcttct gctggtttac cagctacttg gcatcatttg gaatcaagtt atccagacgg
     7981 aaaaatagat atagagactg aactacagct cttggcccag catgatcgga tcattttcca
     8041 gtttccgttt tactggtata gttctcctgc tcatttaaaa atttggcaag ataatgtctt
     8101 ggaacgagca gcacatgtat taaaagggaa agaactagga atcgtattga caacaggtgt
     8161 ggcggaaaaa gaataccaag ctggaggaaa agaagaatat acgatttccg aatttttgag
     8221 gccgtatcag cgaatcgcaa ataaattcca tatgacctat cttccgccat tcgttttagc
     8281 acaatttatg tatttatccc aggaaaaacg ttgggagaag ctaattgctt atcagcagta
     8341 tctctcattg gaaggcaaac catcgctgac tcagcggatt gactggttta ttcaaagagt
     8401 acaggaaaat cagaaaatgc aggaagaaga atctgaaaaa caagcatata tcgtagaagc
     8461 actgactgag gcaaaagagc agatcgaaga cttgaccttt acactgcaag aaatgaaagg
     8521 aacttcatta tgacaaatga ttggctgatc caacaattag atgaattaag cgaaaaatca
     8581 gaatatcaag accgtgcgct cttatatgaa ataaagaaac tgatggctga gatagacgaa
     8641 cgaacaatgc agctgcaagg agaaatcgac ggacaatctt ggaatcatct caactggtga
     8701 aattttctta taaataacaa agataaaaat gtgcacagcg acaattattt gctacaataa
     8761 gaatatgaag cgtttgataa cgctattata cataaaaatt ggtggtgaaa aaaatgtatg
     8821 atgaaaatga gtggggagaa aatgttcgga tgacccgaag aagacagcaa aaacaggcag
     8881 ctcgtaagcg gatgatcatg attggggcag cagttgccgt gtttttgatc atagcaggag
     8941 gagttgcggg tctatatttt catggaaaaa attcgtcaga agcgaaaagt acgaccataa
     9001 cggaaacaac gacaaagaaa acagagacga aacaaacaag tgagtcatca actaaaacaa
     9061 aagagacaca aacttctgaa gaaaaaacag caaatggttt gccagagcaa cttcaagcaa
     9121 tcgtaaatga ttattcgaaa aagatcgaag aaaagacgcc tgcattgatt gaagaatacc
     9181 agacagaaat tcagggaaat caagagggaa tagctggttt atcagcagtt gctaaccaaa
     9241 aagcacgtga gcttcaagca atctccgacg aagggatcag aaaattacgt gcagcgtatc
     9301 aggcagcaga aaataaagac ggtgtagact tagatacttt gatcaatcaa ttatcggcta
     9361 attatacgaa tcatgtcgct aagatttccg atatttattt gcagactagc gcctctcttc
     9421 aagcagagtc aacaagcaca caagacacaa catcttctga ttctgagaca gcagagtcgg
     9481 tagaagcttc tgaaatgcaa gctcgaacaa cacaagacac aacagattcc tctgaaacta
     9541 ataccaacag cgcgacttca caggctgcga caaccgtagt tttggaagga gaaggtccaa
     9601 atcaaatagc cgaacgaact ggcgtaccag tagaaacgat cttgtcgctg aatgggatga
     9661 gcatggagaa ttatttcctg actcccggtg agacgctaaa attgaattga taaaagcaat
     9721 aaaaaagaac gaagcttcca ctaaggtgaa gcttcgttct ttttttattt tccggttctt
     9781 ttttcgcgta caaagtaatg gatcgtcatg taaagcaaga aaacgagtcc aataatcaat
     9841 gagattctcg tttcaggatt gataaacatg aaaatcaata cgatagctaa aaaagcaagt
     9901 gtcacataat tcgtataagg agatagtggc attttaaaag gatgatcatg cattttatct
     9961 gcgtgcttct ttttgaagcg aatctcgctg atcaaaatca ctacccaagg aaccatacca
    10021 ggcaaaatac ttgagctgta tacgaaaacg aaaatatttt ctgactggtt gatgaacatt
    10081 ggtagaagat aatttaagat cagaccaatt aagattccac ctgagattgc tacgacagga
    10141 taaaaaggcg caccgtgacg agatagtttc agaaattttt tagacatctt tccatttatg
    10201 gcaagtgtat agaccatacg acttgcgctg aaaatccctg aattgcaacc tgataatgcc
    10261 gctgtaatca cgacaaaatt aatcagtcct gcagcaaatg taatgccgac tttagaaaaa
    10321 gtttgtacaa aaggtgaacc aatctcactc aattgattcc acggatagat actcacaatg
    10381 ataaaaatag caccaatata aaaaatcaag attcgaccga tagtggactg gacagcttca
    10441 atgatcgtct ttttaggatt ctctgcttca ccagcagtca ttccgatcag ttcgattcct
    10501 tgataggaag caacaacgat cgataatgca aagaaaaatc ctttgacgcc tccagtgaag
    10561 aatccaccat ttttccaaag atttgaaatc cctactgcct gaccatgatt accaaatcca
    10621 aataagatta ccccaagacc tgcaatgatc atcaaaatga tcgttatgac tttgatgatc
    10681 gaaaaccaaa attctaattc gccaaacgcc ttgacagaaa taagatttgc cagcataaga
    10741 aacaagacga caatgattcc gggaataatt gccggcaaat caggccacca ataattcata
    10801 taagatccta cagcaatcac ttcacttatt cctacgacaa tatattgaaa aatattactc
    10861 caagcggtca agtagccagc cacaggatgg atgtactcgc tcgcgtaatt tgcaaaagaa
    10921 ccagtcgaag gatcaacgta aagcatttcg cctaaagcac gcataatcaa atacaaaaag
    10981 aatcctgcaa tcgcataggc tcccatcact gagggccctg tccaacgtat ggttgaggag
    11041 gcacccataa acagaccgac accgatcgtt ccacctagtg cgatcatttg gatatgacgg
    11101 gcactaagac ttcgagttaa cttttcttct tccatacaaa aactcctttc gttgttgagt
    11161 caacaagaaa aaataatcaa acgtttcaac acatgttttt cattattagg ttagccttat
    11221 tgaactcttg atgtgattct acagaaaatt ttcataaaat gcgaggaaaa ttcgaaaaaa
    11281 actttttcat cattctactt gaaaatgctt tccattttag ttaaactata tttaaataaa
    11341 aagttaggta aacctaaaaa ggagtgtaag atttggaacg tacggccatt gtaattcaag
    11401 acctttcagc cgcttatcaa gggaaaactg ttttgaaaac tatcaacttg acgataaatc
    11461 cacaaaaaat taccggtatt atcggaccaa acggtgcagg aaaatcaaca tttatgaaat
    11521 ctttgctaga gttgatacca gctactactg gtaaagtcac cttttctgga cagccggtaa
    11581 actcagtccg taaaaaaata gcctacgtgg aacagcgaag tgaattggat ctttcctttc
    11641 caattgatgt attaggcgtt gtacttttag gaacatatcc atctttacga attggacaaa
    11701 gacctgggaa aaaagaaaaa gaacgtgcaa gacaagcttt gaaaaaagta gggatggaag
    11761 aatatgccaa aagacagatc agcgaactat cgggtggaca gcttcagaga gtttttattg
    11821 caagagctct agcccaagga gcagaatgga tctttttaga tgaaccattc gtagggattg
    11881 atgcgttaag tgaacgaaag atctttgaca tcttgcagga attgaagaat tcaggaaaaa
    11941 cgattttgat cgtccatcat gatcttcata aagtagaaga atatttcgat gaggttattc
    12001 ttttaaataa acagctgatc gcttccggtc cagtacaaga agtctttaca tcgaagaacc
    12061 ttcaattggc ctatggtgag atcatcagac atttagtaaa aggaggagag aaaaaatgat
    12121 ccaatccttt attgatggat taatgaatta tcaattcctg caatacgctt tggtcacttc
    12181 catgcttgta ggacttgctt ctggagtcat cggttctttt attattttac gagggatgtc
    12241 tttgatggga gacgctatct ctcacgcagt tcttccaggt gtagccattt cttatatgtt
    12301 tggttttagt tacatctttg gagccactgc ttttgggatg ctaacagcag cagtgattgg
    12361 tttcgtgact cagcggagcc gattgaaaaa tgatacagcg attggaattg tctttagctc
    12421 cttttttgca ttagggatta tcttgatttc atatgcccag agtgcgacag atttgtatca
    12481 tattttattt gggaatgtct tagctgttag ggaaagcgat ttgttgctga cagcactcgt
    12541 tagtggaatc gtcttgatat ttgtcttttt cttttataaa gaattgaaga tcacatcatt
    12601 cgatccgaca atggcaaaag cttatgggct gaacacatcg ctgatccatt atcttttgat
    12661 gttctttttg acattagtgg ctgtagtcag tttacagaca gtaggaacaa tcttggtgat
    12721 tgccatgttg atcacaccag ccgccacggc ttacttgcta acgaaccatt tactgaaaat
    12781 gatcattaca gctgcaggaa tcggtatgct aagtgcagtt gtcggtgtgt ttttcagtta
    12841 tagttacaat tggccatcag gagctacgat cgtgttagca tgtaccgcat tttttatcct
    12901 tgctttttta ttttctccaa caaaaggaat tttatttcag aaaggaagca gatagatgaa
    12961 aacaaaaaaa tcactctttt taatcttggc agttagtttc ttggtattag ccggttgcgg
    13021 aaagcaggct tctgaccaag cggatgaagg aagtaaagaa aagttgtcag tcgtggctac
    13081 caattcgatc ttggcggaca tggcaaaaga ggtcggaaca gatcaaatag atatccacag
    13141 tatcgtacca gtcggaacag atccgcatga atatgaagta ctaccagaag acatcaaaaa
    13201 ggcaagcgat gcagatgtta ttttatacaa cggtttgaat cttgaaacag gtaacagctg
    13261 gttcgataac ttgatggaaa cggctaaaaa agaagaagga aaagattatt ttgcagttag
    13321 caaaaatgta gaacctcttt atttaactag cggtgaagaa catacaaaag cagatcccca
    13381 tgcttggcta gacctatcta acggaataaa atatgtggag gaaatcgcac gtatattctc
    13441 tgaaaaagat gcagaaaatg cgacactcta taaaaaaaat gcagaagcat atgtggaaaa
    13501 actaaaagaa ttagatacac aagcgaagga aagttttgct tctatcgaag agaacaaaaa
    13561 attattagta acaagtgaag gggcattcaa gtatttttca cgagcatatg atttgccagc
    13621 agcttatata tgggagatca atacagaaag tcaaggtacg cctgatcaaa tgaaagcgat
    13681 cattgatcag ataagagcaa aagaagtacc ggttttattc gtggaaacca gtgtagactc
    13741 aagaagcatg gaacgggtag caaaagaaac gggtttgaaa atctatgata aactgttcac
    13801 tgattccata gcaaaagaag gagaacaagg agattcttac tatcagatga tgaaatggaa
    13861 tattgaaacg atccatgaag gtttgtcaca aacaaaagaa agctgaaaag tgcaaggtct
    13921 agcttgagga gcagaccttg cattttttat aagagatatg aaaaaaaatt atgctgtttt
    13981 tcgtcgtaca gatacctttt ttgaagaaat tccgttatag ttagagtaag gaaggaaggt
    14041 gtggctatga attttacacg taaagataaa tatacgatta aagaagaagt ctttcgattg
    14101 atcagccagc aggaaaatac cgacaaggat caagaccttc aatttcttga agcagaatta
    14161 gctaaattat tcatagcaaa tcaaaatgag acaacagaac cagatgaaga gctgtacata
    14221 ggcaaatggt tgtttgatga atttcagcct ttagatgaaa ataccggtat cggttatttt
    14281 gtgttccttt ctccagtcgg cttcacacct ggcgtattag ttaaacaaaa cgacgaaata
    14341 accgtcaaag catggcctgc attattttca gtcatcaaag cagaaggagt aatcaaaaag
    14401 atccagtcgg cctattataa aagtcaattt attgccaaca gcaaattaat gaatatgttt
    14461 tataatcgtc tagtagaaat tgctaggcct gggaaaatcc cgcaaacatt cacgtacaca
    14521 cagcaaaaaa atctctatga agaagtattg tattcccgcg atctgtctga gttttcgcca
    14581 tgggaagagg taaggctcca tgaactgtta agtacaaaac cagtttcatt gaattttttt
    14641 aatgcattag ataacaaaag aaaattaatt tcaacaaatt attgggaagt atttgctgca
    14701 agaaaaaaat ggatggaaga aaagaagatt gccgatcagc agatccagtt tgtccagctg
    14761 aaaaaagaaa aactatatta tgggttatac agccgttaac agcaagcgag gaagaccatg
    14821 acattaaaag aagaaatcat acaagaaagc aaacgattag gaattgacaa aatcgggttc
    14881 accacggcgg aaccttttga ttcattaaaa gaatcgttgg aagaacaaaa agcagctggc
    14941 catacttctg gctttgaaca tccaaatatc gatgagcgtc tttatccaga aatgacattt
    15001 gatcagccga aatcgatcat ttccattgca cttgcgtatc ctactaggat ccatgaagaa
    15061 gtgcctaggg atgaaaaacg cgggcagttt gctcgtgctt cttgggggat cgattaccat
    15121 gatatactca gagaccgttt gaaccgattg atcgaattca tccaaacaag agcagaaaaa
    15181 tggcaaaaag aagaggaatg gcgtctagct ccgcaagtag atactggaga actgatcgat
    15241 gtcgctgtag cacagcgagc tggtttagga tttattggac gaaacggatt gttgatcact
    15301 gaagagttcg gttcatttgt ctatcttggt gagatcgtta ctaatattgt atttgagcct
    15361 gataaaccag gagaatttgg ttgcggtgac tgtatgcgct gcgtgactgc ttgtccgaca
    15421 aaagcattgc ttggtgacgg aagaatgaat gcccagcgat gtttgtctta tcaaactcag
    15481 acaaaaggga tgatgccgga agaatatcgt aaaaaaatgc gaaatgtcat ttatggatgt
    15541 gatatctgcc agttagtctg cccttacaat caaggaaaag attttcattt tcatgaagag
    15601 atggaaccaa aagtagacga agtatatcca aaattaaagc caatgctttc gatgtctaat
    15661 aaagacttca aacagcaatt tgggcattta gctggttcat ggcgtgggaa gaaacctttg
    15721 cagagaaacg cattgattgc tttagctaat ctaggtgatc gaacggcatt gcctgagatt
    15781 tcagaatgtt taacagatgt ccgccctgtc atcagaggaa cagcagcttg ggctattgga
    15841 cgtctaggaa caaaagaacc ggaaaaatgg cttgatgtat tagatgaaat attagctaaa
    15901 gaaacagaag aagtcgttat aaacgaattg gaacatgccc agaaattatt gcggagaaag
    15961 ttaaaataaa aaaatagtta acattattca aagtttttgt ccagtaattg ttgtaacaat
    16021 tactggtttt tttgtatttt ttacattttt ttaagagaat ttgataaaaa tcaaaaattt
    16081 ttttgctttt ttttatggat tattgcagtg aatgttacag aatctgcgtt tttcattcaa
    16141 aaataaaaaa acatagaaaa acgattactt tgcttataca acaaggcttt tatggttttg
    16201 tatcaaaaaa atatttgttt aaatgttaca aatattaaat aattcgttat tttgtcatta
    16261 aatgtcatgt ttcttttacc tatgaaatat ttctaaatga taaagttaat agcgtcgaaa
    16321 gaataaaaaa cggagaaaaa aaggagttta atatgaattc atttaagaaa attgttttag
    16381 gtacaacttt tgctgctggt gctactgcaa tgtttgtagg aactacaaat gctcacgctg
    16441 atgaagttta tacagtaaaa tcaggtgatt cattatctaa gatctcacaa aaatttgcag
    16501 gagacaactc aatgatcgat gcaattgctg aaaaaaattc gatcgcaaac atcaaccgca
    16561 tttatgttgg tgaacaatta acaatcccaa cttcaaatga ttcttcagca acaacagaaa
    16621 acaaaacaac agaaaataca gcatctacaa ctgaaacagc aacgcaagaa catacatacg
    16681 ttgcacctgt tgaaacagtt gaagtagctc ctgctgcccc agctgctgca acagcgccaa
    16741 cttcaagctc tgcaaaagaa tggatcgcac aaaaagaatc aggcggttca tacacagcta
    16801 ctaacggacg ttacatcggc cgttatcaat tagatgcttc atacttaaac ggcgactatt
    16861 cagctgccaa tcaagaacgt gtagcagaac aatatgtaac atctcgttat ggttcatggg
    16921 atgctgctaa aacattctgg ttagcaaacg gctggtacta agccgataaa aggaaaatga
    16981 tgaaaccgag aaacttgttt attcaagttc tcggtttttt tg
//
LOCUS       DACEEZ010000060        16819 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20313_2017
            SAMN12639966-rid9879223.denovo.060, whole genome shotgun sequence.
ACCESSION   DACEEZ010000060 DACEEZ010000000
VERSION     DACEEZ010000060.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639966
            Sequence Read Archive: SRR10023158
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 16819)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16819)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741683.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741683.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:44:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,016
            CDSs (total)                      :: 2,962
            Genes (coding)                    :: 2,889
            CDSs (with protein)               :: 2,889
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 36 of 73
            Pseudo Genes (incomplete)         :: 29 of 73
            Pseudo Genes (internal stop)      :: 18 of 73
            Pseudo Genes (multiple problems)  :: 8 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16819
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639966-rid9879223.denovo.060"
                     /isolate="E20313_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(<1..455)
                     /locus_tag="IWO89_001987"
     CDS             complement(<1..455)
                     /locus_tag="IWO89_001987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor"
                     /protein_id="HAQ0456715.1"
                     /translation="MVMNAISAMKKTKQQLHEAGFKLTPQREATLLVLLENEKEHLSA
                     EEIFFLVKKKSPEIGLATVYRTLEILTELKIVDKVSFNDGLARYDLRKEGAKHFHHHL
                     LCLNCGTIEEVEEDLLLEVEQVVEERYHFLVKDHRLTFHGICQECYEKE"
     gene            complement(560..1423)
                     /locus_tag="IWO89_001988"
     CDS             complement(560..1423)
                     /locus_tag="IWO89_001988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HAQ0456716.1"
                     /translation="MNDLLATIFTGIIIDENEKNYFVQKNGQTFGLSKSEGEHKIGEA
                     VEGFGYQNQKHENRITTQIPKSRIGHYAFGIVTATRKDLGAFVDIGLPDKDFVVSLDE
                     LPTMTELWPKKGDRLMISLRVDAKDRIWGSLADEKIFKSLSKYGTEEMKNKDVIGTAY
                     RLKLTGTYVLTEDFYLGFIHPSERYNEPRLGEQIKGRVIGVRPDGVLNLSLKPRAYEA
                     ISDDAQMILTILERSADQQINFTDKSDPEEIMKAFGISKGAFKRALGNLLKQGLITQE
                     NGVTKLVKKSN"
     gene            complement(1619..3718)
                     /locus_tag="IWO89_001989"
     CDS             complement(1619..3718)
                     /locus_tag="IWO89_001989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TFIIB-type zinc ribbon-containing protein"
                     /protein_id="HAQ0456717.1"
                     /translation="MKKCPNCGSKKMEQNTICPDCGHQITAEDQQIKESIEETNTDAA
                     MQDKSTEDAVFPDTELNDPIEWSELKDLPLESVMELFEKAEPEDEEPPAHDQVTNIEK
                     NEQKKATVHETSEESGKNQEETLESKQKRRVAELKEIVDNEEENSILSAYIKAHREDT
                     KEEHAKELLKMISEKIAQENKVEPEKEQETEKDSDRENATGKETVKIKDVKTDITEKT
                     EVFTEKQLEKEKTNKLPEAEKKEILENTDKKSTEPDEINSESDAENQSVSKEGTDLEA
                     KIEKEENQPKLFEAEAKSITIVPDTEKTHQESNLEKTDPDKAQKKKSKKVPYLVLAAV
                     LLLGGGSWAYYDHHQKVEAEVAAEAKRQQQMEELHEKLAAFYTDDHHQFIRADMIHQD
                     LSKLKNDLSSLKNEKGYSELETTYQDIQSKIRDIQKVNEWFIAPAIVDDHLADDIKLK
                     ADKEIQAIETDNTDFGKLIQQAQTEAKNQYAELQTAKEKTAIVFKDGKVVDSATRDQY
                     KTAKEAVEKVKNTDLSAQLNEQLKQVDKMLTDKEKKEEEEKKKAEEAKKAAEEQAKQA
                     AQAAAAAQAAQQAAVAQAAQQEAASSTPSSSNSANQPIKSARASDIADASNPAWTWAP
                     GVQSKVIATCIQRGYIVEGGYRLEKARIENGEGYYNLYATSTKSALMKGIGESALPFY
                     IVTINCKTGWFGGNGSN"
     gene            complement(3938..5047)
                     /gene="rpoD"
                     /locus_tag="IWO89_001990"
     CDS             complement(3938..5047)
                     /gene="rpoD"
                     /locus_tag="IWO89_001990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD"
                     /protein_id="HAQ0456718.1"
                     /translation="MANGTDKKTYEAAVAAFIKENKPKRQVVYDDLSNQLATPFTLNA
                     DEMDKLIQKIEDAGISVVDENGDPSIHSLKSAEKKAEQAKTEDLSAPTGVKINDPVRM
                     YLKEIGRVSLLTAEEEVALALKIEEGDQEAKQRLAEANLRLVVSIAKRYVGRGMQFLD
                     LIQEGNMGLMKAVEKFDYRKGFKFSTYATWWIRQAITRAIADQARTIRIPVHMVETIN
                     KLIRIQRQLLQDLGREPTPEEIGAEMDLPTEKVREILKIAQEPVSLETPIGEEDDSHL
                     GDFIEDQEATSPAEHAAYELLKEQLEDVLDTLTDREENVLRLRFGLDDGRTRTLEEVG
                     KVFGVTRERIRQIEAKALRKLRHPSRSKQLKDFLE"
     gene            complement(5071..6912)
                     /locus_tag="IWO89_001991"
     CDS             complement(5071..6912)
                     /locus_tag="IWO89_001991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HAQ0456719.1"
                     /translation="MAQRIPQEVIETVRSQTNIVEVIGQYVQLKKSGKNYLGLCPFHE
                     ERTPSFSVAEDKQIFHCFGCGKGGNVFTFLQELEGLSFPEAVVKVADFEQIPLEDQWR
                     QRTIAVQNPESATGKLIAAHEKAAEIYHHMLLHTKAGQPALEYLQSRGLSLELIEEFN
                     IGFAPNERSFLQQVFKNESISNELLERSGLFVTHDDGRMSDRFYQRVMFPLRNSQGQT
                     IGFSGRWLAPEKGQEKDQPKYLNSPETELFNKRQVLFNLDKARSDIRKNGEILLFEGF
                     MDVIAAWQAGIKTGLASMGTSLTAQQIQQMEKLTKELVFCYDGDNAGFEATNRGIELL
                     RQNSRLALSVVVVPEKLDPDEYLRKYGEASFQELVAHGRETVFTFKSRYLKQGKNLDN
                     EKDKIEYLEELLVELSRVVSPIEQDMYLSQLSTEFQIGRETIQKQLRDLRRTNQQNRQ
                     EPQESQHTQVTRQETARKKRPLTQVEKAEQILLYRAFKEVSVRNILKEREFQFIHDAY
                     AEVYFLFDAYVSQHYEFNLAEFLDFLQDENLRRLVVEIEYLPVAEESSPREIQDLLDV
                     ISKSGLADEITLKKQMQQEARRTGNKQLELELTIEIINLTKQLKQAK"
     gene            complement(7094..9487)
                     /locus_tag="IWO89_001992"
     CDS             complement(7094..9487)
                     /locus_tag="IWO89_001992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HAQ0456720.1"
                     /translation="MFLILEHARLKHVIIFESWRKMEKKKMNGLTQQEVVQKMASGQQ
                     NDYQEDASKSTKQIFSDNILTLFNFLNFGIGICLLFVGAYSNMAYLAIIVVNIVIGIY
                     QEIHARNLVRKLSIVSQSEVLVIRDGKEQSISTKELVLGDLVKIGAGEQIPADLQVIS
                     GRAEANEALLTGESDLIVKETGAELLSGSFLTSGSVMAEVIRVGADNYAVRLTSEAKT
                     HKAIRSELVDSIRKVSKFTSFVIIPLGIILFLEALFIRDASIKISVIASAAALLGMLP
                     KGLVLLISIALTTGVTKLAKKRILVQDMYSIETLAHVDTLCLDKTGTITEGKMSIQRV
                     EALHPLYEKAIPEIMGTYLAESSDNNITMRAMRDYFELSNRYDVTNKVAFSSDRKWGA
                     MEFPELGVVYVGAPERLVEEDRLPDSLKTAQANGYRVLMLGIAPNQQLNEEKPAFVEP
                     VALFEIDDPIRKNAQETLAYLHEEGVDLKVISGDNPFTVSNIARRAGLPNYDAYVDLS
                     QITEEMEVREAAHRYTVFGRVSPQQKKLLVNELKESGRTVAMTGDGVNDVLALREADC
                     SIAMAEGDGATRQIANLVLLDSDFTTLPEVLFEGRRVVNNVTKVSGIFFIKTIYSFIL
                     SIICAVTAIGFPFIPIQITLIDLAIEGYPSFFLSFEQDKRKITYRYLPTALINALPNA
                     LLVVVNIIAVYLLGKTFAWGNAETTTLMYYLLIGISCMAVVKACYPFNPLRIFLAVTT
                     IVGIYVAAMLFHGILEVNLGLGESVGYFFVFMLVNIVLRVLYWKADIQNQLLKRVAN"
     gene            complement(9594..10454)
                     /locus_tag="IWO89_001993"
     CDS             complement(9594..10454)
                     /locus_tag="IWO89_001993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF368 domain-containing protein"
                     /protein_id="HAQ0456721.1"
                     /translation="MEKRQKMENQEVGYTTKDWMLRFVKGMFIGSGFILPGVSGGALA
                     AVFGMYERLISFLAHLTKNFKENVLFFLPVGLGGVTGIFLLSFLVSFLLGAYETTILW
                     FFVGCIAGTVPALWKEAGKKGRDTSDVIIMVVTFVLAYLFLLYGAQLFDGQVDQNFGT
                     WLIAGGLIGLGMIVPGLSPSNFLVYMGLYKAMADGFKEVRLEVIVPIGIGGILCVLGL
                     SKIMDYIFSKAYSKLFHFILGVVFASTIMIIPTDYAGLGVAGVLACPVLFIAGTALGW
                     WMSRLEEQYK"
     gene            complement(10513..11034)
                     /locus_tag="IWO89_001994"
     CDS             complement(10513..11034)
                     /locus_tag="IWO89_001994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase SLT domain-containing protein"
                     /protein_id="HAQ0456722.1"
                     /translation="MESYQIEITTAAKNYHMENYENLIKAIILTESKGKGTDLMQSSE
                     SLHGEAEIIDNPADSIDQGVKYLAEMIVEAKKQGCDLWTAVQAYNFGKDYIAYVKNHG
                     GENTVELAEAYSKDVLSPLLGNEEGKQYRYWRIQSLLYNGGYLYHNGGNMFYPDIVKM
                     NEKIIETYEKVFK"
     gene            11354..12073
                     /locus_tag="IWO89_001995"
     CDS             11354..12073
                     /locus_tag="IWO89_001995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HAQ0456723.1"
                     /translation="MKKEKKYAKMPDGSEIYYEKSGQGFPLFLLHGNDGSGRFFSEQV
                     PVLERYYTVYLVDSRGHGRSTNEASMLNFQLMAEDLNTIMLLEKIDQADFLGFSDGAN
                     LALVFASSFPKKVHRLILNSGNTLVKGVRFSARVISNIHYAWVWLLSLFRPSLRKNLL
                     VIKLLLHDIGLTENDLKKINSPTLIIVGKKDVIKLKHSLYIAKTIPKASFVLVKEQGH
                     ELARKDPERFNREVLQFLSET"
     gene            12099..14690
                     /gene="mprF"
                     /locus_tag="IWO89_001996"
     CDS             12099..14690
                     /gene="mprF"
                     /locus_tag="IWO89_001996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional lysylphosphatidylglycerol
                     flippase/synthetase MprF"
                     /protein_id="HAQ0456724.1"
                     /translation="MLKNTIQWLKNHLGLFKTIFLISVIVIIVGELMSIGKTLSIQQL
                     GETFATIPVWKSGLMLLIGLISVLPMVGYDIILNRMLEQKQNPRYLFETSWLINTINN
                     IAGFGGFVSVGLRSELYGQKKESRNVIQALSKVFLFLMAGLSIYSLLSFLLVVFTPVA
                     PFLKQYWIWLIGGGLYFPVVFLFTTFKKKGFLGGTSLKTQGQLLLVSLLEWTGVLFSF
                     LSVGYLMEIRIDLWQTIPLFIAASVIGIVSMIPGEIGSFDVMMIIGLSAIGVPREIVV
                     VWILLYRLFYYIIPFLIGIVFFFKNIGSTFDQRYSGIPKQLATEIAHKIVVVLLYFSG
                     IMLVLSATIPQAFTEFRWLHSLNPLKFHFIIQFPSILLGFLLIIMGRGIAARVKRAYL
                     PTIFLIVLALLYVLLSDFSFTPVVFLSILLLIILASKNELFREQLIYSWEWRTIDGII
                     IGALSLLYIVIGVYNLPDFPHRRHHFISFFLFPSEKIWFSGLLAIIAVSFMIVLFVHF
                     LQGEKKQIGEAFNEEKALKILTTYGGNSDSQLIFLKDKRMFAYEKDGEPTVLLQFACF
                     NNKCIVMGDPSGKKEDFPEAIEAFIEETDRLCYLPVFYETSEEIVMILHEFGYDFIKM
                     GEEAYVDLNSFTTSGKKMKGTRAVLNRIEREGFTFDVLQPPFSTEQMSTFKNISDNWL
                     GSRKEKGFSLGFFSEDYLQRAPIAVVKNTQEEIVAFATIMPTYTNNKVGTIDLMRYDS
                     KKAPSGSMDFLFLHLFAYMKEEHIQWFNLGMAPLSNVGTSRKSFLQERIAYLVYEFGS
                     HFYSFHGLREYKSKYATNWVSRYTLYSRDSWIAYVMIALLIIDNAPVEQTQGRISGLK
                     KWIRRKY"
     gene            complement(14878..16065)
                     /gene="tuf"
                     /locus_tag="IWO89_001997"
     CDS             complement(14878..16065)
                     /gene="tuf"
                     /locus_tag="IWO89_001997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HAQ0456725.1"
                     /translation="MAKEHYDRSKPHVNIGTIGHVDHGKTTLTAAITTVLGKKGLANP
                     QDYASIDAAPEERERGITINTAHVEYETEKRHYAHIDAPGHADYVKNMITGAAQMDGA
                     ILVVSATDGPMPQTREHILLSRQVGVKYLIVFLNKVDLVDDEELIDLVEMEVRELLSE
                     YGFPGDDTPVIKGSALKALQGDPDAEAAIMELMDTVDEYIPTPERDTDKPLLLPVEDV
                     FSITGRGTVASGRIDRGAVRVGDEVEIVGIKPETQKAVVTGVEMFRKTLDYGEAGDNV
                     GVLLRGIQRDDIERGQVLAKPGSITPHTKFKAEVYVLTKEEGGRHTPFFNNYRPQFYF
                     RTTDVTGTITLPEDTEMVMPGDNVTIDVDLIHPIAVENGTTFSIREGGRTVGSGIVTE
                     IEA"
     gene            16433..16789
                     /locus_tag="IWO89_001998"
     CDS             16433..16789
                     /locus_tag="IWO89_001998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YxeA family protein"
                     /protein_id="HAQ0456726.1"
                     /translation="MKKLIGVLVVLGIFLGGSFAAYHYFYGGEAYYTKITTNGEMSAT
                     QADNGEKFTTYTYKQAAYNKDGEKKQVTLREEREHPLKMNAYLKLKVNPRKGVLSWEE
                     IKASEVPKNAAEKIDN"
BASE COUNT         4617 a         3490 c         2946 g         5766 t
ORIGIN      
        1 ttttcttttt catagcattc ctgacagatt ccatggaacg ttagccgatg atcttttact
       61 aaaaaatgat aacgttcttc tactacttgc tccacttcaa gtaaaagatc ctcttctact
      121 tcctcgatcg tgccgcagtt caagcatagt aaatgatgat ggaaatgttt ggcgccttct
      181 tttctcaaat cataacgggc taatccatca ttaaaactta ctttatcgac aattttcaat
      241 tctgtcaaga tttcaagggt tcgataaaca gtagcaagtc caatttccgg actctttttt
      301 ttcactaaaa agaagatttc ttctgcagat aaatgctctt tctcattttc caaaagcaca
      361 agcagagtag cttctctttg cggtgtcagc ttaaaccccg cttcatgtag ctgctgtttt
      421 gtttttttca tcgccgaaat agcattcatg accatttact caaccccaac aatttataat
      481 aattacaaat aaaaaaagat ataacactat gttaattaaa attattataa gctagttaag
      541 aaaaacatgc aagggattct caattagatt ttttgactag ttttgtcaca ccgttttctt
      601 gtgtaatcaa tccttgtttc aaaagattac ctaatgcgcg cttaaatgct cctttgctga
      661 taccaaaagc tttcatgatt tcttctggat ccgatttatc tgtaaagttg atttgctgat
      721 ctgcagaacg ttctaaaatg gttaaaatca tttgtgcatc atctgatata gcttcatatg
      781 ctcttggctt caatgaaaga ttcagtacac cgtctggtcg gacaccaatc actcgacctt
      841 ttatctgctc gcctagacga ggttcattat atcgttcaga aggatggata aacccaagat
      901 agaaatcttc cgttaaaacg tacgtacctg ttaatttcaa gcggtatgct gttcctatca
      961 catctttgtt tttcatttct tctgttccat atttgcttaa agatttgaat attttttcat
     1021 ctgctaaact gccccaaata cggtcttttg catcaactct aagggaaatc atcaagcgat
     1081 cccctttttt aggccatagc tctgtcattg tcggcaattc atcaagagat acgacaaagt
     1141 ctttatctgg aagaccgata tcgacaaacg cacctaaatc ttttcgtgta gcagtaacga
     1201 ttccaaaagc gtaatgaccg atacggcttt taggaatttg tgtagtgatc cgattttcat
     1261 gtttttgatt ttggtaaccg aatccttcaa ctgcttcacc gattttgtgt tcaccttctg
     1321 atttgcttaa gccaaaggtt tgtccgtttt tttggacaaa gtagtttttt tcattttcat
     1381 caataataat acctgtaaat atagttgcta ataaatcgtt catttttttc tccctctata
     1441 gtgaaaggtt ttcttaatac atttctttgc tatcataaca taatgttatc tacttgaata
     1501 gctgagcctt ggtttggaaa ggtgaaaaaa ggagtatgac aacagttgtg tcatactccc
     1561 ctttttcgaa taaacggtgt taaacggcag gctcacagcc tctttcattt gcctaaaatt
     1621 aattgctccc atttcctccg aaccatccag ttttacagtt gatggttacg atgtaaaatg
     1681 gcaaagcact ttctccaatg cctttcatca atgcagattt tgttgaagtg gcatatagat
     1741 tataatagcc ttccccattt tcgattctgg ctttttctaa tctgtaacct ccttcaacga
     1801 tatatccacg ttggatacat gtagcaatca ctttgctttg gacacctggt gcccacgtcc
     1861 atgctggatt agatgcatcg gcaatatcac ttgctctggc agatttgatt ggctgattcg
     1921 ctgagtttga agaggaaggg gtcgaggatg ccgcttcttg ctgtgctgct tgtgcgaccg
     1981 cagcttgttg agcggcttga gctgcagctg ctgcttgagc ggcttgtttt gcttgttctt
     2041 cagctgcttt tttcgcttct tctgcttttt tcttttcctc ttcttctttt ttctctttat
     2101 cagtgagcat cttatctact tgtttcaatt gttcattcaa ctgagcagat agatctgtat
     2161 ttttcacttt ttctactgct tcttttgccg tcttgtattg gtcgcgtgtt gctgaatcca
     2221 ctacttttcc gtctttaaag actatagctg ttttttcttt agctgtttgc aattcagcat
     2281 attgattttt agcttctgtt tgtgcttgtt gaatcaattt tccaaaatct gtgttatccg
     2341 tttcgattgc ttggatctct ttatctgctt tcaacttgat gtcatctgct agatgatcat
     2401 cgacgatggc aggtgctatg aaccactcat tcactttttg gatatctcgg atcttagatt
     2461 ggatatcttg gtaagttgtt tctaactctg aatatccttt ttcattctta agactggata
     2521 aatcattttt tagttttgat aaatcttgat gaatcatatc tgcacgaata aattgatggt
     2581 gatcatctgt ataaaaggca gcaagtttct catgaagttc ttccatttgt tgctgtcttt
     2641 ttgcttctgc tgctacttcg gcttctactt tttgatgatg gtcataataa gcccagcttc
     2701 ctccaccgag tagaagtact gctgctaaaa cgagataagg tacttttttt gattttttct
     2761 tttgcgcttt atctggatct gttttttcta agtttgattc ttgatgtgtt ttttcagtat
     2821 ctggcacgat tgttattgat ttcgcttctg cttcaaacag cttaggctgg ttctcttctt
     2881 tttctatttt tgcttcaaga tcagtaccct ctttgcttac agattgattt tcagcatcgg
     2941 attcactatt tatttcatcg ggctctgttg actttttgtc tgtgttttcg agtatttctt
     3001 ttttctctgc ttccggcagt ttattcgttt tttccttttc taattgtttt tcagtaaata
     3061 cttcagtttt ttctgtgata tccgttttta cgtcttttat ttttactgtt tctttacctg
     3121 tggcgttttc tctatcagag tctttttcag tttcttgttc cttttctggc tctactttgt
     3181 tttcctgtgc aattttttca ctgatcattt tgagcagctc tttcgcgtgc tcttcttttg
     3241 tatcttcacg atgtgctttg atataagcgg atagtatcga attttcttcc tcattatcga
     3301 cgatttcttt cagttcagct actcttcttt tttgttttga ttcaagcgtt tcttcttgat
     3361 tctttcctga ttcttccgaa gtctcatgaa cagttgcttt cttctgttca tttttctcta
     3421 tattagttac ttgatcgtga gcaggtggtt cttcgtcttc tggttctgct ttttcgaata
     3481 attccattac agattctaga ggtaaatctt tcaattccga ccattcgatt ggatcgttta
     3541 attctgtatc aggaaagact gcatcttcag ttgatttatc ctgcatagct gcatctgtat
     3601 ttgtttcttc aattgattct tttatttgct gatcttctgc agttatttga tgaccgcagt
     3661 ctggacagat agtattctgc tccatttttt tcgatccgca atttggacat tttttcatca
     3721 cttagcgatc ggctccttta ctaggtttac gctaccattt aatctatcat attctatacg
     3781 tcacgccaat agcttatttt taggatttac ttaaaaataa tgaatgaatt tccttcctat
     3841 agaaaaaaaa gcttgagact gcgtagtctc aagctttttt tcttttaaaa taaaccaaac
     3901 agttttactg atttctcttc tggtcaatag tccacgctta ttctaagaaa tcttttaatt
     3961 gttttgaacg agaaggatgg cgcaattttc ttaatgcttt agcctcgatc tgacgaatac
     4021 gttcccgagt aacaccaaat actttcccta cttcttccaa tgttctcgtc cggccatcat
     4081 ctaaaccaaa acgtaagcga aggacattct cttcccgatc agtcaatgta tctagtacat
     4141 cttccaattg ttcttttagt aactcataag ctgcgtgttc tgctggactt gttgcttcct
     4201 ggtcttctat gaaatcacct aagtgagagt catcttcttc accgattggc gtttcaaggg
     4261 aaacaggttc ttgcgcgatt ttcaagattt ctcggacttt ttctgttgga agatccattt
     4321 ccgcaccgat ttcttctggc gtaggttctc ttccaaggtc ttgcagcaat tgacgttgga
     4381 tacgtatcaa tttattaatt gtttcaacca tatgaactgg aatacggatc gtacgtgctt
     4441 ggtcagcgat tgctcgagtg atggcttgac gaatccacca tgtagcatag gttgaaaact
     4501 taaatccttt gcggtagtca aatttttcaa ctgccttcat cagtcccatg tttccttctt
     4561 ggatcaagtc aaggaactgc atgcctcgtc ctacataacg tttagcgata cttactacta
     4621 aacgaaggtt tgcttctgcc agacgctgtt tcgcttcttg gtcgccttct tcgattttca
     4681 atgctaacgc tacttcttct tccgcagtta aaagggagac acggccaatt tctttcagat
     4741 acatacgaac cgggtcattg atttttacac cggttggcgc agaaagatct tctgtttttg
     4801 cttgttcagc ttttttctca gcactcttca agctgtgaat tgatggatca ccattttcat
     4861 ccaccacact gatacctgcg tcttcaatct tttggatcaa tttgtccatt tcatctgcat
     4921 tcaaagtgaa tggtgtagca agctgattgg ataaatcatc atatacaact tgtcgtttgg
     4981 gtttgttttc tttgatgaaa gctgctactg ctgcttcata cgttttttta tctgttccat
     5041 ttgccataaa agaaggcccc ccttgagtag ctatttggct tgttttaact gtttcgttaa
     5101 attgatgatt tcaatcgtca attcaagctc aagctgtttg tttccagttc gtctggcttc
     5161 ttgctgcatt tgttttttta atgttatttc atcagctaag ccagatttgc tgatcacatc
     5221 tagcaagtct tggatttctc gtggcgaact ttcttctgcc accggtaaat attcgatttc
     5281 caccaccaac cgccttaaat tttcatcttg caaaaagtct aaaaactctg caaggttgaa
     5341 ttcataatgt tgtgagacat atgcatcgaa taaaaaataa acttctgcat atgcatcatg
     5401 aataaactga aattctcgtt cttttaaaat attacggaca gaaacttctt taaatgcacg
     5461 atataataga atctgctctg ccttttctac ttgcgtcaaa ggtctctttt tccgtgcagt
     5521 ttcttgtctc gtaacctgag tgtgttgact ttcctgaggt tcttgacgat tttgctgatt
     5581 tgtccttcga agatcgcgca gttgtttttg tatcgtctct ctgccgatct gaaactctgt
     5641 tgacagttga gatagataca tatcttgttc gattggtgaa acaactcggc taagttcaac
     5701 tagtaattct tcgaggtatt cgatcttgtc tttttcatta tctaaatttt tcccttgttt
     5761 taaataacgt gatttaaaag taaagactgt ttcgcgccca tgagccacta attcttggaa
     5821 agatgcttct ccatacttac ggagatattc atcaggatca agcttttctg gaacgactac
     5881 gacagaaagt gctaatcgac tgttttgccg aagcaattca atccctcgat tggttgcttc
     5941 aaaacctgcg ttgtctccgt catagcaaaa gaccaattct ttcgtcagct tttccatttg
     6001 ttggatctgt tgagctgtta gacttgtccc catagaagct aatccagttt tgataccagc
     6061 ctgccatgca gcaatcacat ccataaaccc ttcgaacaac aaaatttctc cgtttttccg
     6121 gatatcgctt cttgctttat caagattaaa taaaacttgt cttttattga acagttctgt
     6181 ttctggacta ttcaagtatt tgggctgatc tttttcctgc cctttttcag gtgcaagcca
     6241 gcgtcctgaa aaaccgatcg tctggccttg agaattacga agtggaaaca tcactctttg
     6301 ataaaaacga tcagacatac ggccatcatc atgcgtgaca aacaatccag agcgttctaa
     6361 caattcattt gaaattgatt cattcttgaa tacttgctga agaaaagaac gctcattagg
     6421 tgcaaaacct atgttgaatt cttcaattaa ttctaaagac aacccacgac tttgcaaata
     6481 ttctaaagct ggctgtccag cttttgtatg caatagcatg tgatgataga tttccgctgc
     6541 tttttcatga gcagcaatca gtttccccgt agcagattca ggattttgga cagctatagt
     6601 acgctgacgc cattgatctt ctaacggtat ctgttcgaag tctgccactt ttaccactgc
     6661 ttctggaaaa cttagacctt ctagttcttg aagaaaagta aacacgtttc cgcctttacc
     6721 acaaccaaag caatgaaaaa tctgcttgtc ttctgcaaca gaaaaagacg gtgtacgttc
     6781 ttcatgaaaa ggacacagcc ccaagtaatt tttaccagac tttttcaact ggacatattg
     6841 tccaatcacc tcaacgatat tcgtttgact gcgtactgtt tcgataacct cttgaggaat
     6901 ccgttgagcc attttttcac cccctgctct attgatatct tttgggttta acagaagata
     6961 tttcaggaga ataaccggta aaaaatcgca ctaactgaaa tcagtgcgat tttaaaggac
     7021 acaattatac atgtttgatt gtatcatatt tcattgcttt ttcaagtata acacatttct
     7081 atctcattaa gcatcaattt gctactcttt tcagtaactg attttgaata tcagccttcc
     7141 agtacaatac tcgaagaaca atatttacga gcatgaatac aaagaagtat cctacgctct
     7201 ctcccaaccc taagtttact tccaaaattc catggaacag cattgcagct acatatatcc
     7261 ctacgatcgt cgttactgct aggaagatgc gcaatgggtt gaagggataa caagctttta
     7321 caactgccat gcaactgatc ccaatcagca agtaatacat caaagtagta gtttcagcat
     7381 ttccccatgc gaatgttttt ccaagtagat acaccgcaat gatattcact actacaagca
     7441 aggcatttgg taaagcatta ataagtgctg taggcaaata acggtaagtg atttttcttt
     7501 tatcttgttc aaaagataaa aagaaggaag gatacccttc aatcgctaaa tcaatcagcg
     7561 tgatttggat agggataaac ggaaatccaa ttgctgtaac ggcacatata atcgataaaa
     7621 taaatgaata aatcgttttg ataaaaaaga tgcctgagac tttcgtcaca ttgttgacca
     7681 ctcggcgtcc ttcaaacagg acttctggca atgtagtaaa atcagagtcc aacaatacca
     7741 gattcgcaat ctgacgtgtt gctccatcac cttctgccat agcaatgctg caatctgctt
     7801 ctcttaacgc caaaacatca ttcacaccgt cgcctgtcat tgcaaccgtt ctgccgcttt
     7861 ctttcaattc atttaccagc aatttctttt gctgcggact cactcgtcca aaaaccgtat
     7921 agcgatgagc agcttctcgc acttccattt cttcagtaat ttgagaaaga tcgacatacg
     7981 catcataatt cggtaatcct gcacgtctag cgatattgga gacagtaaaa ggattgtctc
     8041 cagaaatcac tttcagatcc acaccttcct catgaagata agctagtgtt tcttgagcgt
     8101 tttttcggat tggatcatcg atttcaaaca gagcaaccgg ctcaacaaat gctggttttt
     8161 cttcgttcaa ttgctgattt ggagcgatcc ccaacataag tacgcgatag ccattcgctt
     8221 gggctgtttt tagactgtcc ggaagccgat cttcttccac caaacgttca ggcgctccta
     8281 cgtacacgac tcccagttct ggaaattcca tcgcgcccca tttgcgatca gaagaaaatg
     8341 cgactttgtt tgtcacatca taccgattag aaagctcaaa ataatctctc atagcccgca
     8401 tagtgatgtt gttatcactg ctttctgcca gatacgtccc cataatttct gggatggctt
     8461 tctcgtacag cggatgtagt gcttctaccc gctggatact cattttccct tcagtaatgg
     8521 tccctgtttt atccaagcat aatgtatcga catgggccag cgtttcaatt gaatacatgt
     8581 cttgaacaag gattcgtttt tttgccagtt tggttacccc tgtcgttaat gcaatactta
     8641 ttagcaatac caaacccttt ggaagcatgc ctaacaaagc tgctgcagat gcaataacag
     8701 aaattttgat agaagcatcg cggatgaaca gtgcttccaa aaatagtatg atccccaaag
     8761 gaataatcac gaaactagtg aatttggata ctttgcgaat cgaatctaca agttcagaac
     8821 gaatagcttt gtgagtcttt gcctcgctag tcaagcggac agcataatta tctgcaccta
     8881 ctcggataac ttcagccatg acagaaccgc ttgtcaaaaa gcttccagaa agcaattcag
     8941 ctccagtttc tttgacaatc aaatcagatt cgcctgtcaa aagagcttca tttgcttctg
     9001 cccgtccaga tataacctgt aaatcggctg gtatctgttc tcctgctccg atttttacca
     9061 aatctcctaa taccagctct ttagtagaaa tcgattgttc tttaccgtcc cggatcacta
     9121 gtacttctga ttgggaaaca atcgataatt tccgtacaag atttcttgca tgtatttctt
     9181 gataaattcc aataacgata ttgacgacaa taattgctaa atacgccatg ttggaatacg
     9241 cacctacaaa taacagacaa atcccaatac caaagttcag aaaattgaat aacgtcaata
     9301 tattatcgct gaatatttgt tttgtacttt ttgatgcatc ttcctggtag tcattttgct
     9361 ggccagaagc cattttctga actacctctt gctgtgtaag tccattcatt ttctttttct
     9421 ccattttccg ccaactttca aaaataatca cgtgtttaag tctagcgtgt tccaagatta
     9481 aaaacaactt gtttttataa aatttataga aaaacacacg aatgatttta caaaaaagat
     9541 gccaaccttt ttgtttgaat cacccgtgtg ctgtttttat tatcaatcta tctttactta
     9601 tattgttcct ccaaacggct catccaccaa cctagtgctg taccggctat aaaaagaaca
     9661 ggacaagcta gtaccccagc aactcccaat cccgcgtaat cggtaggaat gatcataatc
     9721 gttgaagcaa aaactacacc taaaatgaaa tggaatagct tgctatatgc ctttgagaaa
     9781 atgtaatcca tgatttttga caatccaaga acacaaagaa tcccaccaat tccgatcggc
     9841 acaatcactt caagtcgtac ttctttaaat ccgtcagcca tcgctttata caatcccata
     9901 tacaccaaaa aattagaagg gctcaatcct gggacgatca tacccaaacc aatcaatccg
     9961 ccggcaatca gccatgttcc aaaattctga tcgacttgcc cgtcaaataa ttgagcgccg
    10021 tataataaaa acaaataagc cagaacaaaa gtaacaacca tgatgatcac atcagaagta
    10081 tctcttcctt ttttccctgc ttctttccat aacgcaggaa cagttccagc gatacatccg
    10141 acaaaaaacc ataatatcgt tgtttcatac gcacctaata aaaaactgac taaaaaagaa
    10201 agtagaaaga tgcctgtcac tccgcctaga ccaacaggca gaaaaaacaa aacattttct
    10261 ttgaaatttt ttgtaaggtg ggctaaaaaa gaaatcagcc gctcatacat gccaaagaca
    10321 gctgctaatg caccaccgct gactcctggc aagataaatc ctgaaccgat aaacattccc
    10381 ttcacaaacc gtaacatcca gtctttggtt gtgtacccca cttcttgatt ttccattttt
    10441 tgcctttttt ccactatttg cgcactcctt ctttcattta attttattca tgattgaaca
    10501 tttcctgcta ttttacttaa agactttttc ataggtctct attatttttt cattcatttt
    10561 gacaatatca ggataaaaca tgttacctcc attatgatag agatagccgc cattgtaaag
    10621 caaggactgg atccgccaat atcggtattg tttcccttcc tcatttccta aaagtgggct
    10681 cagcacatct ttagaatacg cttctgctaa ttctactgtg ttttctccgc catgattttt
    10741 tacataggca atataatctt tcccaaaatt atatgcttgg acagctgtcc ataaatcgca
    10801 tccttgtttc tttgcttcta ctatcatttc cgccaaatat ttgacacctt gatcaatact
    10861 gtcagctgga ttatcaatga tctcagcttc tccatgcaga ctttccgagc tctgcatgag
    10921 atctgtccct tttccttttg attcggtcaa tattatcgcc ttgatcaaat tctcgtagtt
    10981 ttccatatga taattttttg cagctgttgt tatttctatt tgatagcttt ccactcgttt
    11041 taccgaacga taagttcttc ctcataaaaa caacaccgca ccaataaccg tcagaatgat
    11101 catgtaaaaa agaatacgga taatagataa tcgttttcgc ttcatttcat cacctagtta
    11161 ttttgacatc tttttttatc ttactatttc tttatcttct tggctatttc ttccttgatt
    11221 tctgtgtacg ccttaagaaa aaatgaattt tcctgtattt tttgacatct tacgaaaatg
    11281 atcgttgttt ttcagtaaaa acaattgccc aatgactgat ataatcgtta tactttacac
    11341 agtaggtgat tatgtgaaaa aagaaaagaa atatgcaaaa atgccagatg ggagcgaaat
    11401 ttactatgaa aagagcggtc aaggcttccc tctcttttta ctgcatggca atgacggcag
    11461 cggccgcttt ttttcagaac aggtccctgt actcgaacgc tactataccg tgtatctagt
    11521 agatagccga ggacatggtc gttcgacaaa cgaagcatct atgttgaatt ttcagttaat
    11581 ggccgaggat ctgaatacga tcatgttgct ggaaaaaatc gatcaggcag attttcttgg
    11641 atttagtgat ggagcaaatc tagcactggt atttgccagc agcttcccta aaaaagttca
    11701 tcgattgatt ttaaattctg gaaacacgct tgttaaaggc gttcgttttt ctgccagagt
    11761 catcagtaat attcattatg catgggtatg gttactctct ttatttcgcc caagtcttcg
    11821 caaaaatctt ttagttatca aacttttgct gcatgacatc gggcttacag aaaatgattt
    11881 aaaaaaaatc aactccccca ctttgatcat cgtgggaaaa aaagatgtaa tcaagctaaa
    11941 acattctttg tatatagcta aaactatacc aaaagcttcc tttgtgcttg tgaaagaaca
    12001 aggacatgaa ttagctagaa aagatcctga acgcttcaat cgagaagttc ttcaatttct
    12061 ttctgaaaca taactattag gaatagaagg tgatttattt gttaaaaaat accatacaat
    12121 ggctgaaaaa ccacctagga ctgttcaaaa caatcttttt gatttctgtc attgtcatta
    12181 ttgtcggtga attgatgtcc atcggtaaaa ctctatctat ccagcaatta ggagaaacgt
    12241 ttgccactat acctgtatgg aaaagcggac taatgctatt gattggtctt atctctgtgt
    12301 tacctatggt tggttatgat attattttga atcggatgct tgaacaaaag cagaatcctc
    12361 gttacctgtt tgagacaagc tggctgatca atacgatcaa caatattgcc ggtttcggcg
    12421 ggtttgttag tgtggggctt cgctctgaac tatatggaca aaagaaagag agcagaaacg
    12481 tgatccaagc attatcgaaa gttttcttgt ttttgatggc tggactttct atttacagtc
    12541 tgctttcttt tttacttgta gtcttcacgc cagttgctcc ttttttgaag caatattgga
    12601 tatggttgat cggcggcggt ctatattttc ctgtcgtctt tttatttact actttcaaaa
    12661 agaaaggatt ccttggtggt acctcactta aaactcaagg acagctattg ctagtttcat
    12721 tgttggaatg gaccggtgtc ctgtttagtt tcctttctgt tggttattta atggaaatcc
    12781 ggattgatct gtggcaaaca attccattat tcattgctgc ttcagtgata ggaatcgtgt
    12841 ctatgattcc aggagaaatt gggagtttcg atgtaatgat gattatcggg ttgtccgcta
    12901 ttggtgtgcc aagagaaata gtcgttgtgt ggatcttgct ctatcgcctg ttctactaca
    12961 tcattccgtt tttgattgga atcgtctttt tcttcaaaaa tatcggttcc acttttgatc
    13021 agcgttattc tggtatccct aaacaattag caacagaaat tgcgcataaa atcgtggttg
    13081 tcttattata cttttctgga atcatgctag tactttctgc cactatcccg caagctttta
    13141 cagagtttcg ctggcttcat agtttgaacc cattgaaatt tcattttatt atccaatttc
    13201 ctagtatact attgggattt cttttaatca ttatgggcag agggatcgca gcacgtgtga
    13261 aacgagcata tttgcctaca atcttcttga ttgttctagc tttactttat gttttattaa
    13321 gtgattttag tttcacaccg gtggttttct tgtctattct gcttctgatc attcttgctt
    13381 ctaaaaacga attatttcgg gaacaattga tctattcatg ggagtggcgt acgatcgatg
    13441 gaatcattat tggtgcactg tccttgcttt acatcgttat tggggtatac aatctgccgg
    13501 atttccctca tcggcgtcac cattttattt cctttttctt atttccttct gaaaagattt
    13561 ggttttcggg attgttggcc atcatcgctg ttagtttcat gatcgttcta tttgttcatt
    13621 tccttcaagg agaaaagaaa cagattggcg aagcctttaa tgaagaaaag gccctgaaga
    13681 tccttacaac ttatggagga aactccgata gtcaattgat cttcttaaaa gataagcgga
    13741 tgtttgccta tgaaaaagac ggtgaaccaa ctgttcttct ccaattcgct tgcttcaata
    13801 ataaatgtat tgtaatgggt gatccttctg gtaagaaaga agattttcca gaagcaatcg
    13861 aagcttttat cgaagaaaca gatcgactat gctatttacc agttttctac gaaacaagcg
    13921 aagagattgt tatgattttg catgaattcg gctacgattt cattaagatg ggggaagaag
    13981 cttatgtaga cctgaatagt tttacaactt cagggaaaaa aatgaagggc actcgtgctg
    14041 tcttgaatcg gatcgaacga gaaggattta catttgatgt cttacaacca ccattttcta
    14101 ctgagcaaat gagtactttc aaaaatatct ctgataattg gttgggttca cgaaaggaaa
    14161 aagggttttc tttaggcttt ttctctgaag attatctaca aagagcgcct attgcagttg
    14221 tcaaaaatac ccaagaagaa attgtcgctt ttgcaacgat catgcctact tacacgaaca
    14281 ataaagtagg aacgattgat ctgatgcgat atgattctaa aaaagcacct tctggcagta
    14341 tggatttctt gttcttgcat ttgtttgcct atatgaaaga agaacatatc caatggttca
    14401 atttagggat ggcccctctt tcaaatgtcg gaacttccag aaagagtttt ttacaggaaa
    14461 ggattgcata cttggtttac gagtttggtt ctcattttta ttctttccat ggattaagag
    14521 aatataaaag caagtacgct acaaactggg tctcacgtta tactctatat tctagagata
    14581 gttggattgc atatgttatg attgctttgc tgattattga taatgcgcca gttgagcaga
    14641 cacaaggtag aataagtggt ttgaaaaaat ggatacgaag aaagtattaa aaactgcata
    14701 ccgatagatg aacactctca tgatgaataa aagagactga agtaaaacga aagttcatgc
    14761 ttctacaatc tcttcttata caattgaaga acaaagagcg gtccaaacag aaatcctgtt
    14821 tggaccgctc ttttatttta aacatttttc attaccaaaa tattcgagtc gcgtttctta
    14881 tgcttcgatc tctgtaacga tacctgaacc tactgtccgt ccgccttcac gaattgagaa
    14941 tgtcgttccg ttttcaacag cgattggatg gatcaagtca acatcaatcg tcacgttgtc
    15001 accaggcatg accatttctg tatcttctgg caatgtgatt gttcctgtaa catcagttgt
    15061 gcggaagtag aactgtggac gatagttgtt gaagaatgga gtatgacgtc caccttcttc
    15121 ttttgtcaac acgtacactt ctgctttgaa ttttgtatgt ggagtaatgg aacctggttt
    15181 agcaagtact tgtccacgtt cgatatcgtc acgttggatc ccccgtaaca acacgcctac
    15241 gttatcccca gcttccccgt aatctaacgt tttacggaac atttctacac ctgttacaac
    15301 tgctttttgt gtttcaggtt tgatccctac gatctctacc tcatcaccga cacgaacagc
    15361 accacgatca atacgacctg aggcaacagt tcctcgacct gtaattgaga agacgtcttc
    15421 cactggcaat agtaatggtt tatctgtatc acgttctggt gttgggatat attcatctac
    15481 tgtatccatc aattccataa tagcagcttc agcatctgga tcgccttgca atgcttttaa
    15541 tgcggaacct ttgatcacag gagtgtcatc gcctgggaaa ccatattcgc tcaataactc
    15601 gcgaacttcc atttctacca aatcgatcaa ttcttcatca tcgactaaat caactttgtt
    15661 caagaaaaca atcaggtatt ttacaccaac ttggcgtgac aataaaatgt gttcacgtgt
    15721 ttgaggcatc ggaccgtcag ttgcagaaac aactaagatt gcaccatcca tttgtgctgc
    15781 ccctgtaatc atgtttttca catagtccgc gtgtcctggt gcatcaatat gcgcgtagtg
    15841 gcgtttttct gtttcatatt ctacgtgtgc ggtattgatc gtaatcccac gttcacgttc
    15901 ttctggagct gcatcaatac tagcatagtc ttgaggattt gctaggcctt ttttgcctaa
    15961 tactgtagtg atcgcagcag ttaaagtagt tttcccatgg tcaacgtgtc caatcgtacc
    16021 aatgttaacg tgtggtttac ttctgtcata atgttctttt gccataattt acaaacctcc
    16081 taaataatat agatcactga aaaatttcag ctctcacctt tttattatag tctttttttg
    16141 actaaatcaa aaagaatacg tccgacttac tttttgtaag attcgaacta agcttattat
    16201 acgtcaatat tggtcaaagt caactaagag cactcgtctg tcttatacgc gaaaaaagta
    16261 ccttttataa ataaaaaaat gtgttttttg agaaaaactt caagatagtc cttaactttc
    16321 ttttgaagca ataagaacaa aatgaggaaa agtttaaaat tcagctgttg ccaacaactt
    16381 tcaatagacg aaatcttcta tttcgttaat aataaggaaa gaggtgaagt acatgaaaaa
    16441 attaattggc gtacttgtcg ttttaggcat atttctcggc ggaagttttg cagcttatca
    16501 ttatttttac ggcggcgaag cttattatac aaaaatcacg acgaatggcg aaatgtcagc
    16561 tacccaagca gataacggag agaaattcac gacttatacc tacaagcaag cggcttataa
    16621 taaagatggt gaaaaaaaac aggtgacgct tcgcgaagaa agagaacatc cattaaagat
    16681 gaatgcgtat cttaaactca aagtcaatcc tcgtaaaggt gtattaagct gggaagaaat
    16741 aaaggcttct gaagtgccta aaaatgcggc agaaaaaatc gataattagt aaatggtctt
    16801 aaaattttat tagataaaa
//
LOCUS       DACEEZ010000061        16657 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20313_2017
            SAMN12639966-rid9879223.denovo.061, whole genome shotgun sequence.
ACCESSION   DACEEZ010000061 DACEEZ010000000
VERSION     DACEEZ010000061.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639966
            Sequence Read Archive: SRR10023158
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 16657)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16657)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741683.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741683.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:44:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,016
            CDSs (total)                      :: 2,962
            Genes (coding)                    :: 2,889
            CDSs (with protein)               :: 2,889
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 36 of 73
            Pseudo Genes (incomplete)         :: 29 of 73
            Pseudo Genes (internal stop)      :: 18 of 73
            Pseudo Genes (multiple problems)  :: 8 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16657
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639966-rid9879223.denovo.061"
                     /isolate="E20313_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(221..1177)
                     /locus_tag="IWO89_001999"
     CDS             complement(221..1177)
                     /locus_tag="IWO89_001999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic acid exporter family protein"
                     /protein_id="HAQ0456727.1"
                     /translation="MRIGMRTVKTVISVFFSMVTAGSLSLLYWPAAGIIALLSVGNTK
                     RSTLRTGMNRIAAFILATATALFSFFAVGYTIFGFSLFLFLFIPLASRFKLTEGIVVN
                     SVLVTHYLVEENMSWQLIGNEAGLMAIGLVFALLANVYMPDRTKQLKENQIQIEKEFR
                     NILRQMAEFLLSENKEDVQIKCEHLLTFIRESQEDAREHQENYWLRQPLYYETYFSMR
                     RAQANVIKDMLENLERIQQPAYYGKHIYGLLIYTAETFSESNDGRQILTRIEEVYVLY
                     RQMPLPTSRPEFEDRAELFQFLQSFKSFIEIKAEFSQQKIQK"
     gene            1281..1883
                     /locus_tag="IWO89_002000"
     CDS             1281..1883
                     /locus_tag="IWO89_002000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HAQ0456728.1"
                     /translation="MQFGRFRLGMRTIKSALAVFLCILFFHLTQRGIPMIAALSAVFS
                     LRQDLTTTVSFGKSRIIGNTLGGTLAIIYFFVKSYFHNDFLVELILLPILVIIVIVVS
                     DGINNNSGIISAIATLLLIALSIPQGESALYAIQRVLDTFIGTFIAIGINFFLRPPET
                     EKKEELSEDLVELQKKERLLRTNLQEIEAKIQKQKKNSED"
     gene            complement(1971..3470)
                     /locus_tag="IWO89_002001"
     CDS             complement(1971..3470)
                     /locus_tag="IWO89_002001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flotillin family protein"
                     /protein_id="HAQ0456729.1"
                     /translation="MEIPVVFIAIAIAALILLMLLIVFVAKYQTAKPDEALIISGSYL
                     GSKNVHIDESNNKIKIVRGGGAFVLPVFQRSNRISLLSSKLDVSTPEVYTEQGVPVMC
                     DGTSIIKIGSSVEEIATAAEQFLGKTREELENEAREVLEGHLRSILGSMTVEEIYQNR
                     DKFSQSVQEVASVDLAKMGLIIVSFTIKEVRDKNGYLDSLGKPRIAQVKRDADIAEAE
                     ALKETRIKKAQAEKESQAAELQRQTEIAESLKEKELKLATYKQEQDVAKAKADQAYNL
                     ESARAQQQVIEQEMQIKIVERQKQIELEEKEITRREKQYDSEVKKKADADRYAKEQEA
                     QAQKVKEVTEAEAERFRVEALAEAEANKTRLAGQAEAEAALAKGKAEAEAKQKIANAF
                     KEYGEAAVLSMVIDMLPQLMREAAQPLGNIEKISVVDTGSSSGETSGANRVTNYATNL
                     LSSTQETLKATTGLDLKEIVENLSTRGTTSQVNMYEAPKEAEQNSMKEE"
     gene            complement(3486..4010)
                     /locus_tag="IWO89_002002"
     CDS             complement(3486..4010)
                     /locus_tag="IWO89_002002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0456730.1"
                     /translation="MIGGIALETIYWYTLLICAGLAVLLIIFGDVFDFDGPLDPMLII
                     PWLTFTSLFGFLGERLTDQSSLFLFIASGGISIVLVFLLNFYILMPMKHAESTMSSSE
                     KTLEGQVAVVITPIPVRGMGEIQLKSVTGSISRPACFYSPQEQTAERGTKVLIIEIKE
                     RICYVMPYESMIKA"
     gene            4308..5057
                     /locus_tag="IWO89_002003"
     CDS             4308..5057
                     /locus_tag="IWO89_002003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HAQ0456731.1"
                     /translation="MNRSSVSDKISSIAKIVANDKLIQKKLQTGDPTHAIQGITNEIM
                     IASNLDFIVVMDPEGIRYSHADPSYIGLPFSSHEDVKKVIGGQEFFSEKEGMLGFGFR
                     YFVPIYYDQEIIGAVCVGLTNNTIDNQVKDGQKMIVFSLIIGLSVGIIGAVFLAQKMK
                     KVLLGLEPQEIAEQLSEKTIIENEVDEGILAISSDKTILLINTPAKNIFYQTEQLLDF
                     EVGKKSVIIYMTFCLKRRLSIKRPFGLNPCI"
     gene            5048..5833
                     /locus_tag="IWO89_002004"
     CDS             5048..5833
                     /locus_tag="IWO89_002004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HAQ0456732.1"
                     /translation="MYLNGIETISSVTPLFSNDRFLGAVATLRNRSEVMKLMEELGGT
                     KQYINSMRAQTHEFMNKLHVISGLIDLKKYEEVTHFIQQLNQNYKKEIGQVSSLIKVP
                     AIAGFLLGKINEAKEQSVEMLLESDSFIPKMEMDDSIHSLLQVLGNLLDNAKEAVLQK
                     HERNGQVRIHLNYDTEAEVFIVTVCDGGVGISEEISEKIFTLGFSTKGINRGFGLNLI
                     HSIVKRHGGFLEFSKQTNGGTIVYLELPKKEDQNDDKCPDNRR"
     gene            5802..6518
                     /locus_tag="IWO89_002005"
     CDS             5802..6518
                     /locus_tag="IWO89_002005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HAQ0456733.1"
                     /translation="MMTNVLIIEDDPMVAELNRTFVESIPNMNVIESFRNVEEGKKYF
                     RQKNADLLLLDVYMPGESGIHFLSWLRQQGNDIPVILITAADDIETIKNVIRYGALDY
                     LIKPFTLQRFQLAIEKYLAFSQTIQTTEKADQKLIDQVFNHTTPIHEIPSFIDLPKGL
                     SKLTLKKISAFILKEDTIFSTKELAQKSSLSRISVKKYLNFLVEQDILNERIKYLEIG
                     QPLTCYELTKNGRKKLTDYC"
     gene            complement(6620..7639)
                     /locus_tag="IWO89_002006"
     CDS             complement(6620..7639)
                     /locus_tag="IWO89_002006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactate dehydrogenase"
                     /protein_id="HAQ0456734.1"
                     /translation="MRIACYGVRPNEIDFFEKLNIYHYDLSLYEELLTHDNIVSAKDH
                     DAVLLRGNCIADETNLAKMQEYGIRYVFTRTVGVNHIDLQAAADFGMTVARVPSYSPN
                     AIAELSLTFAMMLLRNTAYTTIRTSFKDFRVDEQMFSREIRNCTVGIIGTGRIGLTEA
                     VLFKGLGAKVIGYDIHQTEAAKKVLTFHSLEELLAKSDIVSIHVPHIPGENDQMINEG
                     FLAQMKKGSILINTARGELQDNEAILRALKSNHLAGFATDVFANETEVFFRSFKPWET
                     IPDPAIQQLVEMYPRVLVTPHVGSNTDEALSNMISTSFENFREIIETGKTKNEVSLPK
                     ARQLR"
     gene            complement(7658..8716)
                     /locus_tag="IWO89_002007"
     CDS             complement(7658..8716)
                     /locus_tag="IWO89_002007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014372913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AEC family transporter"
                     /protein_id="HAQ0456735.1"
                     /translation="MNLFDIFRSTLTDMSIVSAITSTVFIILLGFVCRKKGIFSESVG
                     KILSKVVLTVALPALAFRAFMQDINTELLMQGINVLIWGLVIYIILILVSKPFFLSYK
                     GDKQTTLRVLTIFGSTTFFGTPIVSAIYGPVGVMYSSIFNIGYRIFLYSYGYIKMSGL
                     KMEAKNIKAMFVNPIIIATFAGLFIWLFQGYLPQVSVTGAEGEISQVAFLRIDQTAPW
                     LFQPMDYLAGLSSPLAWLAIGATLGEVSFKKAASEKTSWYYSLVKVVFVPALNIVILA
                     VLTATNILPVNFEALATIVIMMATPAATVAAAYAISFDREALLASNASLLSTIFAVVL
                     TPVWIVVLQMIHGIGLFS"
     gene            complement(8936..10084)
                     /gene="nagA"
                     /locus_tag="IWO89_002008"
     CDS             complement(8936..10084)
                     /gene="nagA"
                     /locus_tag="IWO89_002008"
                     /EC_number="3.5.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-6-phosphate deacetylase"
                     /protein_id="HAQ0456736.1"
                     /translation="MRTFIYADKFFLASGVKHAGYLEITDGLFGEYTKELPEGEVNII
                     DQSGKWIAPGLVDTHIHGYMNHDVMDNDAEGIKVMSEGLLSCGVTSFLPTTLTSSKER
                     LKDVAETIGKMYQEAPGAKIKGIYFEGPFFTEEHKGAQNPSYFGDPDLDTFHEWQEAS
                     GGLIKKIALAPERQGVEEFVKQVTEEGVVVSLGHSNATLEEAQKAVEAGASVFVHAFN
                     GMRGLNHREPGMVGALLSLEHVFSELICDGHHVHPNAAGILMEKAGHDHVALITDCMM
                     AGGMPDGNYNLGEFPVVVKEGTARLESGNLAGSILKLKEAIKNVVDWGLATPEQAVMM
                     ASLIPAISCKIDDTCGKIAQGRDADFIVLNPDMTLEATYLDGVERFHA"
     gene            complement(10160..10996)
                     /locus_tag="IWO89_002009"
     CDS             complement(10160..10996)
                     /locus_tag="IWO89_002009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAQ0456737.1"
                     /translation="METLIESFGTIIKEIRKEQKMTQKMLSHDICSQSVLSRIENNEE
                     LPNVLVMMQICQRLGVTIDHVMNLSRKTTRTNDKLFELLDSCFQKRDYRKLEQVLKSS
                     DISENLYQATDFQRYYYYLGVCEFTLRQNISQSLYYLKEALSYSSQKERIHVSDTEIQ
                     LISCIGKIYGVAGKTAEARYYLSRSIQLLHQSADERSKSEFSQIFYNYGNFLFHQNEI
                     DEALEQVNQGIYWAQEKNSYYYLNDLFVLKSLIYKRKEEPERAAFYEGLAQAVKQISK
                     NL"
     gene            complement(11180..12397)
                     /locus_tag="IWO89_002010"
     CDS             complement(11180..12397)
                     /locus_tag="IWO89_002010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HAQ0456738.1"
                     /translation="MREFEKSNKLEGVSYDVRGPVLEEAERMQEEGISILKLNTGNPA
                     PFGFEAPNEVIRDLIMNARDSEGYSDSKGIFSARKAIEQYCQLKHFPNVTINDIYTGN
                     GVSELITMCMQGLLDNGDEVLVPMPDYPLWTASIALAGGTPVHYICDEEAEWYPDIDD
                     IKSKITSRTKAIVIINPNNPTGALYPKELLEEIVEVARQNNLIIYSDEIYDRLVMDGL
                     EHIPIATLAPDLFVVTLNGLSKSHRVAGFRVGWMVLSGDKSHVKGYIEGLNMLSSMRL
                     CSNVLSQQIIQTALGGYQSVDELLLPGGRIYEQREYIYNAINDIPGLSAVKPKAAFYI
                     FPKIDTKRFNILDDEKFVLDFLHEHHILLVHGGGFNWNQPDHFRIVYLPKMDDLKQTA
                     KKMREFLATYRQK"
     gene            12780..12863
                     /locus_tag="IWO89_002011"
     tRNA            12780..12863
                     /locus_tag="IWO89_002011"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:12814..12816,aa:Leu,seq:gag)
     gene            complement(13103..14473)
                     /locus_tag="IWO89_002012"
     CDS             complement(13103..14473)
                     /locus_tag="IWO89_002012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0456739.1"
                     /translation="MNKHNRKITNNKELILLLIGIWGIFLLFNMYMPTMRADDVVYAS
                     RLDELGYLGASIEHYKTWSSRIIIELFLMFFSKHFMLWKLLNSTIMLGIVVLLCKYVF
                     EKINVKNLLLVCSIYCLIPLTVMGETGWRATTLNYQWPVAFSLLTFYPFFQLLRGEEI
                     NRKIYWVSIPLLIFLTNQEQVNACFFVLTSIVSLYLIVNGRYNYKLSVFSIISLAELI
                     FSLTTPGNALRAAHEINKWFPEYKNFNFLNKLDLGISSFGKPFFLDMNILFLLLFFLI
                     FLLTYRKCQNYYVRILTALPFFLNLIIYFGNTMGQSFTYVNGNKRAMIWSSSNLNNLF
                     TELGTKLSLFYPGTWIATLVVLALLLCLIVGIYLSFDNKKTSIFLVILMIMGFCSRLI
                     MGFSPTVWASGMRTYYILYVVIAILVLMAVKELMKSMSVQKNEFMQFGLTVLGICTFI
                     ITVINR"
     gene            complement(15187..15477)
                     /locus_tag="IWO89_002013"
     CDS             complement(15187..15477)
                     /locus_tag="IWO89_002013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0456740.1"
                     /translation="MEKKYGTVDKIRTLQVSARPLIRFSLNGVNCLIASHSLNFLAEV
                     DEGMKLVVTGYYNNRKQFVVRSYHVLGKPKIVVEYEKSLYPRKKVRQDERIN"
     gene            complement(15685..16377)
                     /locus_tag="IWO89_002014"
     CDS             complement(15685..16377)
                     /locus_tag="IWO89_002014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HAQ0456741.1"
                     /translation="MKKKFITGGIVVAFLCLIGYTGTRYANEIILWGGETDIDTINEN
                     LTQLDHTLTDKEQALTDLTARLAQKEQTLQDSKADVQEYQTKVNELENQKNQSAAEKQ
                     NLESQLVSKDAQLQSKQNEINVKLDEINQKNDEINHLNADWTARLQEAQAKLSELQNT
                     VNGLNQQIATLTQEKTNVETQLTDTQKKLDEATAENSSLKQYIQKLEKAKDEVEDTAD
                     KSQQIVEEHLRK"
BASE COUNT         5050 a         3329 c         2780 g         5498 t
ORIGIN      
        1 tggacttatt accgcagagg aaagcttttg aacaccgttt aagaaagagg ttgtgagcct
       61 accgttcagc tccgagtcac aaggagcaat ttcaaaaaac agcttctcat gtttgttgaa
      121 tattgtgact tgtgaccgag gagttgacag gagaacaccg tttaaagaag aggttgtgaa
      181 atcagctttt tgacctgaat attagctgaa ttcacaacct ctatttttgg attttttgtt
      241 gggagaattc tgccttgatt tcaataaaac ttttaaaaga ctgcaaaaac tggaacaatt
      301 ctgctcgatc ctcgaattca gggcgactag tcggcagagg catttgccga tacaatacat
      361 aaacttcttc aatacgtgtt aaaatttgtc tgccatcatt tgactctgaa aatgtttctg
      421 cagtataaat caataaacca tagatatgtt ttccataata agctggttgc tggattctct
      481 ctagattttc cagcatgtct ttaatcacat tggcctgtgc tcttctcata gagaagtaag
      541 tttcgtagta tagcggctgt ctgagccagt agttttcttg atgttctctg gcatcttctt
      601 ggctttcgcg aatgaatgtc agtaaatgtt cgcatttgat ctgcacatct tctttatttt
      661 ctgataaaag aaattctgcc atttgtctca aaatatttcg aaattctttt tcgatctgta
      721 tttggttttc cttcagctgc ttcgtacgat ctggcatgta aacatttgca agcaaagcaa
      781 atactaatcc aatcgccatt aatcccgctt cattgccgat cagctgccaa gacatgtttt
      841 cctccactaa ataatgagtg accaaaaccg aatttactac tatcccttcg gttaatttga
      901 agcgagaggc aagcgggatg aacaggaata aaaaaagact gaagccaaaa atagtatacc
      961 caacagcaaa aaaactgaac agtgctgttg ccgtagctaa tataaaggct gcgattcgat
     1021 tcatgcccgt acgcaatgta gagcgcttgg tatttcctac acttaataat gcaatgatcc
     1081 ctgcagctgg ccaatacaac agcgataagc tacccgctgt gaccatcgaa aagaagacac
     1141 tgatcactgt tttgactgtt cgcattccta tacgcattta cttccccctt ttttatttta
     1201 attatacttg aataatgaaa aactagctat cagacaaaag tttaggtatg atagtaaaaa
     1261 gaccaaaaag gagaatctct atgcaatttg gccgttttcg actaggtatg cgtacgatca
     1321 aaagtgcctt agctgttttt ttatgtatct tgttttttca tttgactcaa cgaggaatcc
     1381 cgatgattgc tgcattatct gctgtttttt ctttgcgtca ggacttgact acaaccgttt
     1441 cttttggaaa gtctaggatc atcggcaata cattaggtgg tacattagct atcatttatt
     1501 tttttgtcaa aagctatttt cacaatgatt ttttggtaga attgatcttg cttcctattc
     1561 tagttattat tgtgatcgtc gtgtcagatg gtattaacaa taattccggt atcatttccg
     1621 caatagctac gttgttgctg attgcgttga gcatccctca aggcgaatcc gctttgtatg
     1681 ctatccaacg tgtactagat acttttatag ggacattcat tgcaatcggt atcaatttct
     1741 ttcttcgtcc tcctgaaaca gaaaagaagg aagaactatc agaagatttg gtcgaactcc
     1801 aaaagaaaga acgattgcta agaacaaatt tacaagagat agaagctaag atccaaaaac
     1861 aaaagaaaaa tagcgaggac taattttctt ttgcaataaa ataaaaaaat ggagagaaga
     1921 actttaaaaa gtcttcctcc atttttgatt gaacggtgaa gtgtactact ttattcttcc
     1981 ttcatcgagt tttgttctgc ctcttttggc gcttcataca tatttacttg gctagtagtc
     2041 ccacgtgtcg acaaattctc tacgatttcc ttcaagtcaa gtcctgttgt cgcttttagt
     2101 gtttcttgag tagaagaaag caaatttgtg gcataatttg tcactcggtt cgctccactg
     2161 gtttctcctg aactgcttcc tgtatcgaca acagagattt tttcgatatt tcctagcggc
     2221 tgtgctgctt cgcgcatcaa ttgcggtagc atatcgataa ccatactcaa gacagctgct
     2281 tcgccatatt ccttgaaggc attggcgatc ttttgttttg cttcggcttc tgcttttcct
     2341 tttgctagtg ctgcttcggc ttctgcttgt ccagctaaac gtgtcttatt ggcttccgct
     2401 tctgccagtg cttccacacg gaatcgttct gcttcggctt cagtcacttc tttgactttt
     2461 tgagcttgtg cttcttgttc tttagcgtac cgatcggcat ctgctttttt cttcacttcc
     2521 gaatcatatt gcttttcacg acgtgtgatt tctttttctt ccagttcgat ttgtttttgg
     2581 cgttctacga ttttaatctg catttcctgt tcgattactt gttgttgtgc tcgggcactt
     2641 tctaaattgt aggcttggtc tgcttttgct ttagcaacat cttgttcttg tttgtaggtt
     2701 gctaatttca gttctttttc ttttaatgat tctgcgatct ctgtttgtcg ctgtaattca
     2761 gctgcttgtg attctttttc tgcttgagct tttttgatac gcgtttcttt tagagcttcc
     2821 gcttctgcta tatctgcatc tcttttgact tgggcgattc ttggtttccc taaagagtca
     2881 agataaccgt ttttgtctct gacctctttg atagtgaaag aaacgatgat caaccccatt
     2941 tttgcaaggt ctacagaagc gacttcttgt acgctttgac tgaacttatc gcgattttga
     3001 tagatttctt ctacagtcat agaaccaaga atcgagcgca agtgtccttc taatacttct
     3061 cttgcttcat tttccagttc ttctcttgtc ttgcctaaaa actgttctgc tgctgtagca
     3121 atctcttcta cagatgaacc gattttgatg atcgaagtac catcacacat caccggtacg
     3181 ccttgttcgg tatacacttc aggggtggag acgtccaatt tactcgataa tagactgatc
     3241 cgattcgaac gttgaaaaac tggcaagacg aatgcgccac ctccacgcac aattttgatt
     3301 ttgttattgc tttcatcaat gtgcacattt ttgcttccta aataacttcc actgatgatc
     3361 aacgcttcat ccggttttgc tgtctggtac ttcgctacaa agacgatcag caacatcagt
     3421 aaaatcaaag ctgcgattgc gatcgcgata aaaacaactg gtatttccat ttaacacacg
     3481 ctcctttaag cttttatcat gctttcataa ggcatgacat aacagattct ttccttgatc
     3541 tcaatgatca atactttcgt tccccgctca gctgtctgtt cttgggggct gtaaaaacaa
     3601 gctggacgac taatggaacc ggtaacactt ttcaactgaa tttctcccat acctcgtaca
     3661 gggatcggtg tgatcacaac agctacttgt ccttccagag ttttttcaga agacgacatc
     3721 gttgattcgg catgtttcat cggcatcaat atatagaaat taagcaaaaa taccaacact
     3781 atcgatatgc ctccactagc aataaataaa aataaagatg attgatctgt taaacgctca
     3841 cctagaaaac caaataagct tgtgaaagtt aaccaaggga taataagcat cggatcaagc
     3901 gggccatcaa aatcgaatac atctccaaaa ataatcaata ggactgccaa tcctgcacaa
     3961 atcagcaatg tataccaata aatcgtctca agtgcgattc cccctatcat atacacgcct
     4021 ccttccattt ctacgtctat cataccaatg tttgaaaatt ctgtatagct tcttttaccg
     4081 actcagtggt gttttttata ggaaggttgt gtttatcgtt gacaatttgt taaatttttg
     4141 ttttcatgtt aaaattttca caataaattc tttcgaggta gcctatgttt ccttttttta
     4201 agaaaaaacc attaaagctc cagaacacga tcatccttgt cgtttgtttc tccacattcg
     4261 tttctttgat tgtttctgcc atattgatca ggaattttat tctttcgatg aaccgatcta
     4321 gtgtgtcaga taaaatctct agcattgcta aaatcgttgc caatgataaa ctcatccaaa
     4381 aaaagctgca gactggtgac ccgacacatg cgattcaagg aataacaaat gaaatcatga
     4441 ttgcatcgaa tttagatttt atcgttgtca tggaccctga aggcattcga tattctcacg
     4501 cagacccaag ttacatcggt cttcctttct caagtcatga agatgtgaaa aaagtgattg
     4561 gaggacaaga atttttctct gaaaaagaag gaatgttagg gttcggcttc cgctatttcg
     4621 tccctattta ttatgatcaa gagattattg gtgcagtttg tgttggctta acaaacaaca
     4681 cgattgacaa tcaagtcaaa gatggacaaa aaatgattgt tttttcattg attattggac
     4741 ttagcgtagg catcataggc gccgtttttc ttgcccaaaa aatgaaaaaa gtactgcttg
     4801 gacttgaacc acaagaaata gcagaacagt taagtgaaaa aacgattatc gaaaatgaag
     4861 tagatgaagg aatcttagcc atctcttctg ataaaactat cctcttgatc aatacacctg
     4921 caaaaaacat tttctaccaa acagagcagc tattggattt cgaagtagga aaaaaatcag
     4981 tgataatcta tatgacgttt tgtttaaaga gacgattgag catcaaacga ccgttcggac
     5041 tcaatccatg tatttaaatg ggatagaaac tatttctagc gttacacctc tttttagcaa
     5101 tgaccgtttt ttaggagcag tcgcaacttt acgaaatcga tcagaagtaa tgaaattaat
     5161 ggaggagctt ggggggacga aacaatatat aaactccatg agagctcaaa cccatgaatt
     5221 catgaataaa cttcatgtca tttctggttt gattgatttg aagaagtatg aagaagtcac
     5281 ccattttatc cagcagctaa atcaaaatta taaaaaagag attggccaag tctcaagtct
     5341 gatcaaagta cctgcaattg ccggtttttt attaggcaaa ataaacgaag ccaaagaaca
     5401 atctgttgaa atgctgttag aaagcgattc ttttatccct aaaatggaga tggatgactc
     5461 catccattca cttttgcaag tgttagggaa cttacttgat aatgcaaaag aagccgtgct
     5521 acaaaaacat gaaagaaacg gacaggtccg gattcattta aactatgaca cagaagctga
     5581 agtattcatc gtgaccgtct gcgatggcgg tgtcgggata tcagaagaaa tctcagaaaa
     5641 aatatttacg ctaggttttt cgacgaaggg aatcaatcgt gggtttggat tgaatctcat
     5701 ccattcaatc gtcaaacgcc acggtggatt tttagaattt tccaaacaaa caaatggcgg
     5761 tacaatcgtc tatctagaat taccaaaaaa ggaagatcaa aatgatgaca aatgtcctga
     5821 taatcgaaga tgatccaatg gttgcagaat tgaatcgtac ttttgtggaa agcatcccta
     5881 atatgaacgt aatcgaaagt ttcagaaatg tagaagaagg aaaaaaatat ttcagacaaa
     5941 aaaacgcaga tctgctgctg cttgatgtat acatgcctgg cgaaagtggt atccattttt
     6001 tatcttggct tagacagcaa ggaaatgata tcccagtgat tttgatcact gctgcagatg
     6061 atatcgaaac cataaagaat gtgatccgct acggtgcctt agattatttg atcaaaccgt
     6121 tcactttgca acgttttcag ctggcgatcg aaaagtatct tgctttctct cagacaatcc
     6181 aaacgactga aaaagccgat cagaaattga tcgatcaagt attcaaccac accactccca
     6241 ttcacgaaat accatcattc atcgatttgc ctaaaggtct gtcaaaactg actttaaaaa
     6301 aaataagtgc atttattcta aaagaagata ctattttttc aacaaaagaa ctggctcaaa
     6361 aaagctcact gtcacgtata tccgtcaaga aatatttgaa ttttttagta gaacaagata
     6421 tattgaacga aagaatcaaa tatttggaaa tcggccagcc gctaacctgt tatgaattga
     6481 caaagaatgg gcggaaaaag ctcacagatt actgttgagt catcgaatta tcccatacaa
     6541 aatagtccaa gtatacagaa gtaacaactg tttacttgga ctatttttaa acattttatt
     6601 gacgaatatc catgttttct tatcttaatt gacgggcttt aggaagagat acctcatttt
     6661 tcgtttttcc agtttctatt atttcacgaa agttctcgaa acttgtcgaa atcatatttg
     6721 ataatgcctc atctgtattt gaacctacat gcggggttac aagaactcta gggtacattt
     6781 ctactaattg ctggatagca gggtctggaa tcgtctccca tggcttgaag gatcggaaaa
     6841 aaacctctgt ttcatttgca aaaacatcgg ttgcaaaacc agctaaatga ttgcttttca
     6901 aagctcttaa aatagcttca ttatcttgaa gctctccacg ggctgtatta atcaagatgc
     6961 tacctttttt catttgagct aaaaatcctt cattgatcat ttgatcgttt tcacctggga
     7021 tatgaggtac atgaatactg acaatatcgc ttttggctaa taactcttca agcgagtgga
     7081 acgtcaatac ttttttagca gcttctgtct gatgaatatc gtagccgatc actttggctc
     7141 ctagtccctt aaacaaaaca gcttccgtca accctatcct gcctgttcct atgatcccta
     7201 ctgtgcaatt tctgatttca cgactaaaca tttgctcatc cactcgaaaa tctttgaaag
     7261 atgttcggat cgtcgtatac gcagtatttc tcaaaagcat catggcaaaa gtcaaagata
     7321 actctgctat cgcatttggc gaatacgatg gtacacgggc tactgtcata ccaaaatcag
     7381 ctgccgcttg tagatcgata tgattgactc ccacagtccg tgtaaaaaca taacgaatcc
     7441 catattcctg catttttgca agattggtct catctgctat acaatttccc ctcaacaata
     7501 cagcatcatg gtcctttgct gacacgatat tgtcatgtgt caacagttct tcataaagac
     7561 tgagatcata atggtaaata ttcaattttt caaaaaaatc tatctcgttc ggacgaacac
     7621 cgtaacatgc gattctcata ctttcttcct ccatttctta tgaaaataaa cctatgccat
     7681 gaatcatttg taatacaacg atccatacag gtgtcagtac cacagcaaaa atagttgaca
     7741 gtaaggatgc attggaggct aaaagtgcct ccctatcaaa actgatagca taggctgctg
     7801 ccactgttgc agctggtgta gccatcatga taacgattgt tgccagtgct tcaaaattga
     7861 ctggtagaat attcgttgcc gtcaaaactg ctagaatcac gatattcaat gcaggtacaa
     7921 ataccacttt gactagactg taataccatg aagttttttc agatgcagct tttttgaagc
     7981 ttacttcacc taatgttgcg ccgattgcta gccacgccaa tggtgaactt aaaccagcta
     8041 aataatccat cggctgaaat agccacggtg cagtctggtc aattcgcaaa aacgctactt
     8101 ggcttatctc tccttctgca cctgtaactg acacttgtgg cagataccct tggaacagcc
     8161 aaatgaaaag gccggcaaaa gtagcgataa tgattggatt gacgaacatc gctttgatat
     8221 ttttagcttc catcttcaag ccactcattt ttatgtatcc ataagaatac aaaaagattc
     8281 ggtagccaat attgaaaatt gaggaataca tcacgccgac aggaccataa atcgcactga
     8341 cgataggcgt tccgaaaaag gtcgtagaac caaagatcgt caggacacgc aacgttgttt
     8401 gcttatcccc tttatagctt aagaaaaaag gttttgaaac tagaataaga ataatataaa
     8461 taactaatcc ccaaatcaag acattgattc cttgcatcag taattctgta ttgatatctt
     8521 gcatgaaagc tcgaaatgcc agtgctggca aagccactgt taagacgact ttcgatagaa
     8581 tttttccaac agattcagaa aaaatcccct tttttctaca aacaaaccct aataatataa
     8641 taaaaactgt tgaagtgatt gcactgacaa tcgacatatc tgtcaaagtc gatcgaaaaa
     8701 tgtcaaataa gttcatcttt ctcctcctaa ttatgttcat ttattcacaa taaaaaaagc
     8761 ttttattttg ctaatacgta ttaacatttt taattttatc tttacattgt tacttattga
     8821 acaataggaa attaaagaaa cttttgaaac ttatgtaaag tgatttttac tagaacacaa
     8881 aaaaacctta aaaccaaact taagaagttt agttttaagg ttaacttaaa ttatattatg
     8941 cgtggaaacg ttccacgcca tctagataag ttgcttccaa tgtcatatca gggttcaata
     9001 cgataaagtc tgcatcgcgt ccttgagcga tttttccaca tgtatcatcg attttacagc
     9061 tgattgctgg aatcagacta gccatcatga ctgcttgttc tggtgttgct aatccccagt
     9121 ctacaacatt tttaattgct tctttcaatt tcaatatact tccagctaag ttgccagatt
     9181 ctagacgtgc tgttccttct tttactacta ctggaaattc gcctaaattg taatttccat
     9241 caggcatccc accggccatc atacagtctg taattaatgc gacatgatca tgtcctgctt
     9301 tttccattag gattcccgca gcatttggat gtacatgatg gccatcacaa atcaattcag
     9361 aaaagacatg ttctaaagat aataacgcac ccaccatacc tggttcgcga tgattcaatc
     9421 cacgcatacc attgaaagca tggacaaata cacttgcgcc tgcttcaaca gctttttgcg
     9481 cttcttctag tgtagcatta ctgtgtccta gagaaacaac tactccttct tcagtcactt
     9541 gtttgacaaa ctcttctaca ccttgacgtt ccggtgctaa agcaattttt ttgattaaac
     9601 cacctgaagc ttcttgccat tcatggaaag tatccaaatc aggatccccg aaataacttg
     9661 ggttttgtgc ccctttgtgt tcttctgtga agaaaggacc ttcaaaatag attcctttga
     9721 ttttagctcc tggtgcttcc tgatacattt tgccgattgt ctcagctaca tcttttaaac
     9781 gttctttgct tgacgtcaat gttgttggta aaaatgaagt cactccacaa gataacagac
     9841 cttcagacat cactttgatg ccttctgcat cattatccat tacatcatga ttcatatagc
     9901 cgtgaatatg tgtatctacc aaaccaggag caatccattt gccgctttgg tcaatgatgt
     9961 tgacttctcc ctcaggtagt tctttggtgt attcaccaaa aagtccatcg gtaatttcaa
    10021 gatagcctgc atgtttgaca cctgacgcta aaaagaattt gtcagcatag ataaaagttc
    10081 tcattctatt ctctcccttt gtttttctac ctctaaagta ctcttattta gagaagaagt
    10141 caagaatggt ctacaccatt tacaaatttt ttgaaatttg tttcacagct tgtgctaagc
    10201 cttcatagaa tgctgctctt tctggttctt ctttacgctt gtatattaag cttttaagca
    10261 caaataaatc attgaggtaa tagtagctat tcttctcttg agcccaatag attccttgat
    10321 tcacctgttc taacgcttcg tcgatttcat tttgatggaa taagaaattt ccataattat
    10381 aaaaaatttg cgaaaattca ctttttgatc gttcatcagc tgattgatgc agcagttgga
    10441 tgcttcgact caaatagtag cgtgcttcag ccgtttttcc tgccacacca tatatttttc
    10501 cgatacaact aatcagttgt atttctgtgt cagaaacatg gattctttct ttttggctag
    10561 agtagcttaa tgcttctttc aaataataaa gggactgaga gatattttga cgcaacgtga
    10621 attcgcaaac accgagataa taataatagc gttgaaagtc agttgcttga tacagatttt
    10681 cagaaatatc agaagatttt agtacttgtt ctaacttccg ataatcacgt ttttggaaac
    10741 aactatccaa cagttcaaat aatttatcgt ttgtccgagt agtttttcga gagagattca
    10801 tcacatgatc aatcgttacc cctagccgtt gacaaatctg catcatcacc aatacattcg
    10861 gcagttcctc attattttcg atccgactta atacactttg tgaacaaata tcatgcgaaa
    10921 gcattttttg cgtcattttt tgttcttttc gaatctcttt aatgatcgtt ccaaaggact
    10981 cgattaacgt ttccataagt atcctcccca aaatatgcgt attcgcattc tattcgtatt
    11041 ttactttagg aagtcgcaaa cttcaagaaa agacttgcaa ctaaataata tactttagtt
    11101 atttaagaaa actccactag aaagagagcc tcagtgtgtt tgtctgtggc cctcctactg
    11161 aaaaatttgg tttttaaaat catttttggc gataggttgc taggaattct ctcattttct
    11221 tagctgtttg tttcaaatca tccattttag gaagataaac gattcgaaaa tgatctggct
    11281 ggttccaatt aaatccacca ccatgtacaa gtagaatatg atgttcatgt aaaaagtcca
    11341 aaacgaattt ttcatcatcc aaaatattga atcgctttgt atcaattttt gggaatatat
    11401 aaaaagcagc ttttggtttt acggctgaaa gaccaggaat atcattgatt gcattataga
    11461 tatattctct ttgttcatag atccttccgc ctggcaggag cagctcatcg acactttgat
    11521 aaccgcctag cgcggtttgg ataatttgct gagacaatac attggaacac aaacgcatgg
    11581 aggacaacat attcagccct tctatatagc ctttgacgtg tgacttatct ccacttagga
    11641 ccatccagcc tactcgaaat cctgctacac gatgagattt ggatagaccg ttcagcgtaa
    11701 caacgaacaa atcaggcgct aatgtagcaa tcggaatatg ttctagacca tccatcacta
    11761 agcgatcata gatttcgtcg gaataaataa tcaggttatt ttgtcgagct acttcaacga
    11821 tttcttctaa taattctttt ggataaagtg ccccagtagg attattagga ttaatgataa
    11881 caatagcttt tgtccgactc gttatttttg atttgatatc atcaatatca ggataccatt
    11941 ctgcctcttc atcgcaaata taatgaacag gtgtgcctcc agctaatgca atagaagccg
    12001 tccacaacgg atagtccggc attggaacca gtacttcatc tccattgtcc agcaacccct
    12061 gcatacacat cgtgatcaat tcgctcactc cgttgccggt gtagatatca ttgatcgtca
    12121 cattcggaaa atgtttcagt tggcaatatt gctcaattgc ttttctagca gaaaagatcc
    12181 ccttggaatc tgagtagcct tctgagtcac gggcattcat gatcaaatca cgaatgactt
    12241 cgtttggtgc ttcaaatcca aaaggtgcgg gatttcccgt gttcaatttt aaaatactaa
    12301 tgccctcttc ttgcatccgc tccgcttctt ctagaacggg accgcgaaca tcgtaactga
    12361 caccttctaa tttgttggat ttttcaaatt ctctcatcac ttagccaatc cctcatttca
    12421 aaaagttaga actttccaaa ttttttgtta tttactattt caatatcata ctccatccta
    12481 gaaaattttg ccacttattt aggaaaaacg taagtattaa aaaaacacaa acaagcgttg
    12541 agaacttaat tgtcatgata gtcattccat agattcgctg cccaatccat tgcaattcca
    12601 caaatgattc ctagcataaa accaagcaaa aaacgtccga atgcaagtcc aatcaataca
    12661 cctaagatca ttcccagtcc tgaaaattct ccgtcatatt tctccatctt aataagtcct
    12721 cccatttttg agcttgacaa ctatgtaaaa cctcgctatc attagtgtgt tattattacg
    12781 cagatgtgtt ggaattggca gacaagcatg attgagggtc atgtgagcgt tagctcgtgt
    12841 gggttcgagt cccatcatct gcactacaac tcgtgaagtt aattttgctt cacgagtttt
    12901 tttgtttgaa cgtttcttta tgccattata tacatatcac tgtatccttt acaaaaagaa
    12961 cgtacaatca tgtaaacagc ctgaggtgag tatatgaagg tcggaaacaa gctactcgta
    13021 gaaacttgca agtgttaaaa caattaatcg aaaaacgagt aaaatccctc acctaatact
    13081 aaagcgaggg attttttgta tattatctgt ttatcaccgt tattataaag gtacaaatac
    13141 caagcacggt taatccaaat tgcataaatt catttttttg tacactcatg cttttcatta
    13201 gctctttaac tgccattagt actaagatgg cgataacaac ataaagtata tagtacgtcc
    13261 gcattccgga agcccatact gtgggagaaa atcccataat taaccttgaa caaaatccca
    13321 ttatcatcaa aataactaaa aaaatagaag ttttcttatt gtcaaaactt aaataaattc
    13381 caacaattaa acatagtaat aatgccaaga ctactaaagt agctatccaa gtacctggat
    13441 aaaaaagtga cagtttcgtt cctaactctg taaagagatt attcaggtta ctactactcc
    13501 aaatcattgc ccttttattt ccattgacgt aagtaaagct ttgacccatt gtattaccaa
    13561 aataaataat caggtttaag aaaaacggta atgcagtcaa tatacgcaca taatagtttt
    13621 gacatttcct atatgttaga aggaaaatta agaaaaatag caataaaaat aagatattca
    13681 tgtctaaaaa aaacggcttt ccaaatgatg aaatacctaa atctaactta ttaagaaagt
    13741 tgaaattttt atattcagga aaccatttat taatttcatg tgcagcacgt aatgcattcc
    13801 ctggagttgt caaagagaaa attaattctg ctaagcttat tattgaaaat actgataact
    13861 tataattata tcgtccattg acaatcaaat ataaactaac tatacttgtt agaacaaaaa
    13921 aacaggcgtt gacttgttct tgattagtta aaaatatcaa taaaggaata cttacccaat
    13981 atatttttct atttatttct tctcctctta agagttgaaa aaacggataa aaagttagta
    14041 aactaaatgc tactggccac tgatagttca aggtggttgc tctccaaccg gtttccccca
    14101 ttactgtcaa tggtattaaa cagtatatag agcaaaccaa taataaattt ttaacattta
    14161 ttttctcaaa aacatacttg caaagtaata ctacaattcc taacataatc gtagaattta
    14221 ataatttcca tagcataaag tgtttagaaa agaacattaa gaatagttct ataataattc
    14281 ttgaactcca tgtcttataa tgttctatcg aagcacccaa atagccaagt tcatctaatc
    14341 tacttgcata aactacgtca tccgctctca tagtcggcat atacatatta aagagtaaaa
    14401 agatacccca aatccctatt agtaacaaga tcagttcttt gttattcgtt atttttctat
    14461 tatgtttgtt catttttata ctccttctta tatttcctaa tagagtataa catagcttta
    14521 agcattactt aaagataact taaaagttat cttttaaata cttaattgtt aaatacctta
    14581 tttgatgcaa aagaaatcta cgatagtcaa caaaaaaatt ttcattatat ccagaaaaat
    14641 ttttcttagt taaggaccta tggattgcag taatgcagaa ttcgtcgaat aaactgccaa
    14701 agacatacaa tcatattgct tttaaaattg acgaacaaga tatagattcg tttgtctcaa
    14761 aaatccaaat gttaggttta actgtcgaac caggacgttc tcgtgtgaaa ggcgaggctt
    14821 cttcaattta cttttacgac tatgataatc acctcttcga gttacatacc ggtactctcc
    14881 aggaacgttt aaaatcctac aattccacta cctgagacta gtaatgacta gtctcttttt
    14941 atggcctctg atccccccat tgagcgaata ctaatagggc ttcgaattga tctgttcaat
    15001 ctttaagcaa tatccctcaa atgagtttgg aaagtttagt gattcatcac aaatacttaa
    15061 atacatgtag ttatatatag attgaggttc ttgtgagcgt atgctcgtgt gggttcaaat
    15121 cccatcgtct gcatccatct ttccaaaaca agcggctagg acatttttgt ctcagccgct
    15181 tgttttctag tttattcttt catcttgtcg gacctttttt cttgggtata gacttttctc
    15241 atattctacc acaattttgg gtttccctaa cacatgatag gatctcacaa caaattgctt
    15301 tctattatta taataacctg taacgactaa cttcatccct tcgtctactt cagctaggaa
    15361 gttcaaacta tgggaagcaa tcaaacaatt gaccccattt aaactgaaac ggatcaacgg
    15421 tctggcacta acttgcaaag tacggatctt atctactgtt ccatattttt tctccataac
    15481 cttcacctca ataccattat acgaacatac attccctttg taaagaagaa actctgtttt
    15541 atctttttga ctttttgtct attacctttc ttaaaatcat attggcactt cctgcgaaga
    15601 tgaaggacat gtactaaaaa aaagaggcag cagtatagat tactgttgct tctttactaa
    15661 tttaggttta cttgttaaga aagattattt acgcaaatgt tcttcaacaa tttgctgaga
    15721 tttatcagct gtatcttcca cttcatcttt tgctttttct aacttttgaa tatattgttt
    15781 caagcttgag ttttcagcag ttgcttcgtc taatttcttt tgtgtatctg ttaactgagt
    15841 ttctacattt gttttttctt gtgtcagtgt tgcgatttgt tgattcaaac catttacagt
    15901 attttgtaat tctgaaagtt ttgcttgtgc ttcttgtagt cttgctgtcc aatctgcatt
    15961 cagatgattg atttcatcat ttttttggtt gatctcatct agtttgacat tgatttcatt
    16021 ttgtttgctt tgtagttgtg catccttgct gactaattga ctttctaagt tttgtttttc
    16081 tgctgccgat tgattttttt ggttttctaa ttcatttact tttgtttggt attcttggac
    16141 atctgccttt gaatcttgca atgtctgttc tttttgtgct agtcgagcgg taagatctgt
    16201 aagcgcttgc tctttatctg tcaatgtatg atcaagctgt gtcagattct cattgattgt
    16261 atcaatatct gtctctccac cccacaaaat aatttcattt gcgtaccgcg tgcctgtgta
    16321 gccaattaga catagaaatg caacgacaat cccaccagta ataaatttct ttttcatttg
    16381 catagctcct taatccaata cttttacatg ctaaaagtaa gtgatctcaa acacaatgtc
    16441 aagcgttttt aaattacatg tgtaatttta atataaatac tatcaaatac tcatttaatc
    16501 ctcctttttt atctatttaa aaaatctttt ttatagagaa aaatgcaggt taatactttc
    16561 ttaaataaat atttatggtg aacaaaaaca aaaaagcaag ttttattaaa cgtaaccctt
    16621 ttcatttcat agattactgt atttattaca aaaaacg
//
LOCUS       DACEEZ010000062        16587 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20313_2017
            SAMN12639966-rid9879223.denovo.062, whole genome shotgun sequence.
ACCESSION   DACEEZ010000062 DACEEZ010000000
VERSION     DACEEZ010000062.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639966
            Sequence Read Archive: SRR10023158
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 16587)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16587)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741683.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741683.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:44:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,016
            CDSs (total)                      :: 2,962
            Genes (coding)                    :: 2,889
            CDSs (with protein)               :: 2,889
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 36 of 73
            Pseudo Genes (incomplete)         :: 29 of 73
            Pseudo Genes (internal stop)      :: 18 of 73
            Pseudo Genes (multiple problems)  :: 8 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16587
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639966-rid9879223.denovo.062"
                     /isolate="E20313_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(79..786)
                     /locus_tag="IWO89_002015"
     CDS             complement(79..786)
                     /locus_tag="IWO89_002015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc metallopeptidase"
                     /protein_id="HAQ0456742.1"
                     /translation="MYGYGMYGFLDPTFLLVILGLVISGIASAYVNSTFRKYDQVRSK
                     NNVTGTQAAQYILQSQQINNVGVQQIAGDLTDNYNSGNKMLSLSQATAQSTSVAAIGV
                     AAHECGHAVQDASGYVPLKIRAALVPAANFGSMISFPLIMVGVLLSWNSTLINIGIFA
                     FSLALLFQLVTLPVEFNASRRAIQILSEGGLLTEEEVPMARHVLFAAALTYVAAALST
                     FLQLLRLILLFGGNRRD"
     gene            complement(865..1203)
                     /locus_tag="IWO89_002016"
     CDS             complement(865..1203)
                     /locus_tag="IWO89_002016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkylphosphonate utilization protein"
                     /protein_id="HAQ0456743.1"
                     /translation="MTLPNCPECGSEYTYEDRGLLICPECGNEWNKEEQETVIEDGLV
                     VLDANGNRLLEGDSVTVIKDLKVKGASGAIKQGTKVKNIRLVEGDHNIDCKVEGFGPM
                     KLKSEFVKKN"
     gene            complement(1314..2276)
                     /locus_tag="IWO89_002017"
     CDS             complement(1314..2276)
                     /locus_tag="IWO89_002017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional oligoribonuclease/PAP phosphatase
                     NrnA"
                     /protein_id="HAQ0456744.1"
                     /translation="MTVQQEIVDTIKKYDKIIIHRHLRPDPDALGSQVGLAELLRESF
                     PEKEIRQVGETIEGLRFLAEMQEVEDVFYQGALVIVTDTANSPRISDQRYQLAEKLIK
                     IDHHPNDDPYGDLVWVNTKASSCSEMIAEFALMFPESLKMNVKAARLLYAGIVGDTGR
                     FLYPSTTSRTLEIASILRNYPFDASALNREIEQMPMKVAKLSGYLYQNIQVDENGAGR
                     IILPQEILDKYGIDDSETASIVSLPGVIDEVLAWGIFVQQPSGYYRVRLRSKGPIINE
                     LAKRHHGGGHPLASGANAKDLAEVEEIYKEIQDICQSYKSRKTE"
     gene            complement(2291..3622)
                     /locus_tag="IWO89_002018"
     CDS             complement(2291..3622)
                     /locus_tag="IWO89_002018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HAQ0456745.1"
                     /translation="MATKHDLILEYIESLPVGNRISVRSIAKELNVSEGTAYRAIKDA
                     ENVGLVSTIQRVGTIRIERKLKKHIERLTFGEVVRIIEGDVLGGAAGLDKVLNKFVIG
                     AMTKHAMERYITPGSLMIVGNRAEVQKLALEDGAAVLITGGFNTTAEIAKLADDLEMP
                     ILRTSYDTFTVATMINRALSDQLIKKDIMLVSDIYMPAEKTHYLQQTDTISDYQRLSE
                     ETQHSRFPVVNRHHRLMGIVTAKDVLGKSPNQIIDRVMTKEPISVKKTMSIASVSHQM
                     IWDGLEVMPVVSDDLTLEGLITRQDVMKAMQLVQRQPQIADTISDQISGNIHTIDTDR
                     EGNRLENPKFKFVVAPQMVNGVGTISFGVLSEIISDVAQKTMVMNQKRNILIEQVNLH
                     YLRLIQLESELDIRPRVLEIGRRSAKLDIEVFIENTIVSKAIVVCQVMERS"
     gene            complement(3630..4310)
                     /locus_tag="IWO89_002019"
     CDS             complement(3630..4310)
                     /locus_tag="IWO89_002019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HAQ0456746.1"
                     /translation="MKITYHGHSCLTIEMENGQRVMIDPFITGNPLTDLTAEEVKTDW
                     LLITHGHSDHIGDMIPIAKENQATIISIVEIADYANSRGVDSFGMNIGGKHAFPFGVV
                     KFVHAQHSSSYEVDGIVQYMGEPSGIIIQAEGKTIYHAGDTAYFSDLGLLAEEFDIDV
                     AFLPIGDNYTMGPEDAAKAAQTIRAKKVVPIHYNTFPVIEQDPESFVELLEDSEGKIM
                     SVSETLEV"
     gene            complement(4529..5008)
                     /locus_tag="IWO89_002020"
     CDS             complement(4529..5008)
                     /locus_tag="IWO89_002020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA deacylase"
                     /protein_id="HAQ0456747.1"
                     /translation="MMSQTKVERYLEEQKISYQPLDFSHVVEESFTEELQKRGIDPAL
                     ICKTLVVKGNKTGVIIALIPLNDHLDYKKTRQVTKDRKVGFPGMDFVLMHTGYPHGAN
                     TPIGIKLAHPDYCFLADSSLKEKEEVIVSSGEIGRSLRIKVADLEKIVHPIYADLIS"
     gene            complement(5135..6814)
                     /locus_tag="IWO89_002021"
     CDS             complement(5135..6814)
                     /locus_tag="IWO89_002021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease J"
                     /protein_id="HAQ0456748.1"
                     /translation="MSTIKIIPLGGVRENGKNMYVAEVGEDIFILDCGLKYPENELLG
                     IDVVIPDFTYLEENIDRVAGVFLTHGHADAIGALPYLLSKISVPVFGTELTIELAKLN
                     VSRNEAAKGFKDFHVVDEHTEIDFGETVVSFFRTTHTIPDSVGINLKTKEGSIVYTGD
                     FKFDQTAIPMYQTDYARLAEIGKEGVLALLSESANAENPQPVASEAQIADEVFDTIKY
                     WEGRIIVACVASNLQRVQQVLDAAEKAQRKVVLTGQDFERIIRTAMRLEKLQLPSEDL
                     LVTLKDMKNYDPEELIILETGRMGEPIKSLQKMANGTHRNVKIEEGDLVYITTTPSIA
                     METIVAKTEDIIYRAGGTVKLISENMRVSGHANPNDLQLMINLMKPTYFVPVQGEYRE
                     LAAHADLAHAVGMPYKNIYITGRGDVLEYKNKRMSVAGTTSAENVMIDGLGIGDIGNI
                     VLRDRKILSEDGIFVAVVTISRREKKIISKPQITSRGFVYVKASRDLMKESSNMIETI
                     VEKHLESNDFEWSKLKQDIRDQLGRYLFEQTKRRPVILPVIMEATQRRNKK"
     gene            7362..8864
                     /locus_tag="IWO89_002022"
     CDS             7362..8864
                     /locus_tag="IWO89_002022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAQ0456749.1"
                     /translation="MNFMKRAWKSTKVKWGRSLLLFAVFTAILIFVLAGLTIRSAAEE
                     AANQAQKEVGATVTLSANREASFKKQEQTSSSGSTTEERPDPGSFSLTPVSLTDAEKI
                     AELDNVKSYSFESSASAMAKDGITAITSSDSSEDSSETTDAGGEPAGGMGGNPQMTQA
                     DFQISGVSATAQNSNFSDGTAKITDGEGIDDSDKGTNNVVIDSTLAETNDLAVGDTFT
                     ITSTEDEDTTYEMTIKGIYESSETSGSMGMKFNFMNPSNTLYTSYTFANELNGTSDDN
                     TIDSAIYTLSDPDKMDEFLKEAEKLIDTDTFSLQSNDSMYQSMLEPLNNVASFSKNVV
                     LLVAVAGIIILTLIIMITIRERRHELGVLLSLGESRSKIILQLFTEVAICMILALGVA
                     SLSGNVVANAVGQQLLDQQTETTTQNQQAPGGGQMPGNRQEQGGQRDGQQGTTNNPFA
                     VSDQVSELEINVKPAQLGLLAGVAFGISLFSVFLASIGILRLNPRKILLN"
     gene            8877..9539
                     /locus_tag="IWO89_002023"
     CDS             8877..9539
                     /locus_tag="IWO89_002023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ0456750.1"
                     /translation="MLRTEQLGFWYQDPQDALYKDVELNFEAGKMYAILGASGSGKTT
                     FLSLISGLDAPKEGKVYYQDRSLNKIGLRSYRKNDVSIIFQAYNLLPYLSALDNVIAA
                     MEITKTKQENYRQVALDRLARVGIDEILAKKQVTKLSGGQQQRVAIVRAICCEHQLIV
                     ADEPTGNLDETTSQDIVKLFQEIAHEQNRCIIIVTHEIEVAKSCDEVFELKNKKFQKI
                     EV"
     gene            complement(9640..9843)
                     /locus_tag="IWO89_002024"
     CDS             complement(9640..9843)
                     /locus_tag="IWO89_002024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HAQ0456751.1"
                     /translation="MADLKGRSKDMKDKVVGEGKEAYGKATDDKSKETEGKAQSLGAD
                     IKEKARNLGDEVKDKFSRKDEKK"
     gene            complement(9954..11138)
                     /gene="iadA"
                     /locus_tag="IWO89_002025"
     CDS             complement(9954..11138)
                     /gene="iadA"
                     /locus_tag="IWO89_002025"
                     /EC_number="3.4.19.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-aspartyl-peptidase"
                     /protein_id="HAQ0456752.1"
                     /translation="MKIIRQIEVFAPDYLGKMDVLIAGGKILAVEEQLEGGYEGVEVE
                     ELSGEGKVLTPGFIDCHFHILGGGGEGGYQNRTPEVTLSQLTTAGVTTVVGCLGTDGE
                     GRDMTALISKAKGLEAEGISTYVYEGSYRLPVKTVTDSIIKDFLTIDKIIGIGEIAVS
                     DHRSSQPSFEEFARAVADARVGGMLSGKAGIINVHLGGGKRKLELLTRIVEETEIPIT
                     QFLPTHANRTPELFEACVAFAKRGGTIDFTASEDPDFWEKTDGEVRFSKALKRLIEED
                     VSLDNFTMTSDGQGSLPYFDENNHFLGLGVGSAKALLVGIKEAVQKESIPLEIALRAI
                     TSNPARILKLDKKGKIEIGADADLCILDKETLDIDTVIAKGEIMVQEKEVKVWGTFEK
                     SF"
     gene            complement(11295..13034)
                     /gene="yfcC"
                     /locus_tag="IWO89_002026"
     CDS             complement(11295..13034)
                     /gene="yfcC"
                     /locus_tag="IWO89_002026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative basic amino acid antiporter YfcC"
                     /protein_id="HAQ0456753.1"
                     /translation="MKKTNSSGKPKRDFTKLSTPHTYVIIFGVVIFAWILTFVVPAGK
                     FSTQDIEYKDANGETSTRTVLRQDSFRYAYELDKSYVFDQLEELQDHPAEREKLDVPE
                     KGLEKVIADGEKNLTQEKLDEISLTDDVLYDQYGENIYDTSKKLHKTAKIWGTDDFGG
                     FGFLNFVFEGLVSGDKYGSAVGIAALILVVGGAFGIIMRTGAIDAGIYAFISKTKGLE
                     RLALPLLFFAFSFGGATFGMAEEVIPFSMVMVPFVIALGYDSIVAVTVTYVASQVGNA
                     TSWMSPFSVAVAQGIAGIPVLSGATFRLIMWVVVTALAAGYMMIYAEKIRKKPESSLT
                     YKSDDYFRSHIKKTSDENKKFMLGHKLILLEMLVVLVWIVWGVTQKGYYIPEIASQFF
                     VMGLVAGIIAVLFKLDGMKVNDIASSFQSGAADLAGTAIVVGMAKGILLVLGGSDASV
                     PSALNTILHGIGTALTGVPAVIGAWAMYIFQSLFNLVVTSNSGQAALTMPIMAPLADL
                     VGVSRQVAVLAYQLGSGFMDAFTPVSASLIGVLGVARIEWAKWARFQIKMQGFLFVLG
                     TIFIMIAIAIGLQ"
     gene            complement(13807..14421)
                     /locus_tag="IWO89_002027"
     CDS             complement(13807..14421)
                     /locus_tag="IWO89_002027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HAQ0456754.1"
                     /translation="MSLCYFDYYHQLEEKLRIEEPLMLQAGTWIAKQINQGGRLQIYA
                     SRTLSGVAFEFWDQTPDLVPSILIENPAGGVYETLEGAGQAIIDQLSVKSEDIFLLLS
                     NEGRNPAIIELAQWIKENGHPLIIVTGFDLSRSIKSQHSSGLRLFEFADLVLDNHANL
                     HDAVLTLPNLDPAICGTASLATIFLLQQVLYFTAKQLVQDTSGK"
     gene            complement(15027..15404)
                     /locus_tag="IWO89_002028"
     CDS             complement(15027..15404)
                     /locus_tag="IWO89_002028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0456755.1"
                     /translation="MGKLVAVAQSLLDILKWLMEGSNWIFVLPLLLISLFIMFRMVRK
                     KETFLIFIWYAASVGVATLMVELALGSWEACVFALFFAVLYLIIYIPYMKRKSRIGRK
                     GRHTTTFSSTDSLEQSRRTRRRR"
     gene            complement(15704..16585)
                     /gene="galU"
                     /locus_tag="IWO89_002029"
     CDS             complement(15704..16585)
                     /gene="galU"
                     /locus_tag="IWO89_002029"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HAQ0456756.1"
                     /translation="MKVKKAVIPAAGLGTRFLPATKAMAKEMLPIVDKPTIQFIVEEA
                     LDSGIEDILIVTGKEKRPIEDHFDANIELENNLREKEKTELLALVEETTQVNLHFIRQ
                     SHPRGLGDAVLQAKAFIGNEPFVVMLGDDLMKDDIPLTQQLMMDYEETQASAVAVMRV
                     PENETSKYGIIDPKTDSEKGRCRVKGFVEKPEMGKAPSNLAIIGRYLLTPKIFEILET
                     QEPGAGNEIQLTDALQTLNQTEAVYAREFKGKRYDVGDKLGYMKTNIEYGLQHSEIGT
                     SLSAYIIQLSKKLRTES"
BASE COUNT         4807 a         3573 c         2832 g         5375 t
ORIGIN      
        1 ttccaaataa atggtattca atcagctaac tctcggtatt agctcagatc aaagcgctga
       61 tttcacaact tctttatttt aatctcttcg gttgccgccg aacaataaga tcaagcgcaa
      121 cagctgcagg aatgtcgaaa gcgctgctgc aacataagtc agtgctgctg caaataatac
      181 gtgtcttgcc atcggtactt cttcttccgt cagtagtccg ccttcactca aaatttggat
      241 tgctcgccga gacgcgttaa attcgactgg taatgtcacc aattggaaaa gtaatgctaa
      301 agaaaaagcg aaaatcccga tattgattaa cgtagagttc cagctaagca atacacctac
      361 cataatcaag gggaacgaaa tcatcgaacc aaagttagct gctggaacaa gtgctgcacg
      421 aatcttcaat gggacataac ccgaagcatc ttgtaccgca tgtccgcatt catgagctgc
      481 tacacctatc gcagcaactg aagtagactg tgcagttgct tgagacaaac tcaacatctt
      541 attacctgaa ttgtagttat ctgtgagatc tccggcaatt tgttgaacac cgacattatt
      601 tatctgttga ctttgtaata tatattgtgc tgcttgtgta ccagtcacat tgtttttgct
      661 tctcacttga tcgtattttc ggaaagtact attgacgtac gctgacgcaa ttccggaaat
      721 aactaatcca agaatgacca aaagaaatgt cggatctaaa aaaccataca ttccataacc
      781 atacattttc gtttcactcc ttttcgttta ctttatttta tcattagtag taggaaaaga
      841 atgtgaaaag accttgaata ttctttaatt ctttttcaca aattccgatt tcagtttcat
      901 cggtccaaat ccttctacct tgcaatcgat attatgatcg ccttctacta aacggatgtt
      961 cttgactttt gttccttgtt taatggcacc tgacgcacct tttactttca agtctttaat
     1021 cactgtcacg ctatcgccct ctaacagacg gtttccattt gcatctaata ccactaatcc
     1081 gtcttctatg acagtttctt gttcttcttt gttccattcg ttcccgcatt ctgggcaaat
     1141 caacaagcct cgatcttcat atgtgtactc tgagccacat tctgggcaat ttggtaatgt
     1201 catcttcttt ctcctttaaa atctttagtg agcactattg taacggaaaa agaagatctt
     1261 gaaaagatac atttgtgatt tttactacaa aaggcacgtt ttcgaacgaa gtatcattct
     1321 gtttttctgc tcttatacga ttggcatata tcttggatct ctttatagat ttcctctact
     1381 tctgctaaat cttttgcatt cgcaccactt gctaatggat ggcctcctcc atgatggcgt
     1441 ttagccaatt cattgatgat cggcccctta gaacgtaaac gtacgcggta ataccctgaa
     1501 ggctgctgaa caaaaatccc ccaagccagc acttcgtcaa tcactccagg aagtgaaacg
     1561 atcgaagctg tctctgaatc atcgattcca tatttatcaa gaatttcttg cggaaggatt
     1621 attcttcctg caccattttc atccacctgg atattttgat aaagataacc ggaaagttta
     1681 gcgactttca tcggcatttg ttcaatctct ctgttcaaag cacttgcatc aaatggataa
     1741 tttctcaata tcgaagcaat ctctaatgta cgggacgtcg tggagggata caaaaagcgc
     1801 cctgtatctc cgacgattcc tgcataaagt aaccgtgcag ccttaacgtt catctttaaa
     1861 gattcaggaa acatcaaagc aaattctgca atcatctcac tacagctact tgcttttgta
     1921 tttacccaca caagatctcc ataagggtca tcattgggat gatggtcgat tttgatcagt
     1981 ttttctgcca actgatatct ttgatcactg attctgggtg aattagctgt atcagtcacg
     2041 atgactagcg ctccttggta gaagacatct tcaacttctt gcatttctgc taaaaagcga
     2101 agcccttcaa tcgtttcacc tacttgacgt atttcttttt ctgggaaact ttcccgtaat
     2161 agttcggcta atcctacttg tgatcctaat gcatcaggat cagggcgcaa atgcctgtgg
     2221 ataatgattt tatcgtattt ttttattgta tctactattt cttgctgtac tgtcatttca
     2281 ctcctgcttt ctaagaacgt tccattactt gacaaacaac gattgctttg gaaacgatgg
     2341 tgttttcaat aaacacttcg atatccagtt tagcggaacg tctgccgatt tccaatacac
     2401 gtggacgaat gtctaattcg ctttccaatt ggatcagccg caaataatgt aggttcactt
     2461 gttcaatcaa gatatttcgt ttttggttca tcaccattgt tttttgtgcg acatcagaaa
     2521 tgatttcgct aagtacgcca aaagaaatgg ttccaacgcc attgaccatt tgtggtgcta
     2581 ctacgaattt gaacttaggg ttttccaaac gatttccttc acggtcagta tcaatcgtat
     2641 ggatatttcc agatatctga tccgaaatcg tatctgcaat ctgcggttgt cgctggacaa
     2701 gctgcatcgc tttcattaca tcttgccggg taatcaagcc ttccaatgtc aagtcatcgg
     2761 aaacgaccgg catgacttct agcccatccc agatcatttg atggctaaca gaggcaatac
     2821 tcattgtttt ctttacactg attggttctt tcgtcatcac ccgatcgatg atctggtttg
     2881 gcgatttccc taaaacatct tttgccgtaa caatccccat caaacgatga tggcggttga
     2941 cgactggaaa acgcgaatgc tgcgtttctt ctgacaaacg ctgataatca gaaatcgtat
     3001 ctgtctgctg caaataatga gtcttttcag ctggcatata gatatcactg acaagcatga
     3061 tatctttttt gatcaattgg tcgcttaacg ctcggttgat catggttgcg actgtaaatg
     3121 tatcataaga ggtgcgtaag atcggcattt caagatcatc agctagcttt gcaatctcag
     3181 cagtcgtatt gaatcctcct gtgatcaata ctgcggcgcc atcttccaat gctaattttt
     3241 ggacttccgc ccggttgccg acgatcatta gtgatcctgg cgtaatatag cgttccatgg
     3301 catgtttagt catcgcgccg atgacgaatt tattcaagac cttgtctaat cctgccgccc
     3361 cgcctaaaac gtccccttca atgatcctga cgacctcgcc aaatgtcaaa cgttcaatat
     3421 gtttttttaa tttgcgttcg atacggatgg tccctactcg ttgaattgtt gaaactagtc
     3481 cgacattttc tgcatctttg attgcacggt acgctgttcc ttcactgaca ttcaattctt
     3541 ttgcgatgct tcttacagaa attcgattgc caacaggtaa actttcaata tattctaaaa
     3601 tcaaatcatg ttttgtcgcc attttctgcc tacacttcca gcgtttccga gacgctcatt
     3661 atttttcctt cactatcttc cagtaactct acaaaagatt cgggatcttg ttcaatgaca
     3721 gggaaagtat tgtaatggat tggaactact ttcttggcac gaattgtttg cgcagccttt
     3781 gctgcatctt ctggccccat cgtatagtta tcgccaatcg gcaagaaagc gacatcaata
     3841 tcgaattctt ctgcaagcaa tcctagatca ctgaaataag cagtatcccc tgcatgataa
     3901 atcgtttttc cttctgcttg aataataatt ccgctaggct cgcccatata ctgaacgata
     3961 ccgtctactt catagcttga actatgctgt gcatgtacga attttaccac gccgaatgga
     4021 aacgcatgtt ttccaccgat attcataccg aatgaatcta ctcctctgct gttcgcataa
     4081 tcagcaatct caacaatact gataattgtg gcttgatttt cttttgcaat aggaatcata
     4141 tctcctatgt ggtcactatg accatgagta atcagcaacc aatctgtctt tacttcttct
     4201 gcagttaagt ctgtcaatgg atttcctgta ataaacgggt caatcatcac gcgttggcca
     4261 ttttccattt caattgttaa acaggaatgt ccatgatacg taattttcat attttacacc
     4321 tcgcgttttc tctgtatctt ttttattaaa aacgatttca tcacttgcac atcaatgaaa
     4381 aaatatagct gctaaatata ttttcattgg attgatagta cagtatgtac aaagcaattg
     4441 taacagataa tacaggatgc accaagtacc aagcagatat aatctcaagt ttgaggtctc
     4501 tgctctgctt agcttctctt ctcgtccctt aactaattaa atcagcgtaa ataggatgga
     4561 cgatcttttc taaatcggct acttttatcc gaaggcttct cccgatttca ccgcttgaca
     4621 caattacttc ttctttttct tttaaagaac tgtctgctaa aaaacaataa tcaggatgtg
     4681 cgagttttat tccaataggg gtgtttgctc cgtggggata tcctgtatgc attaatacaa
     4741 aatccattcc aggaaaacca actttacgat ctttcgtcac ttgtctcgtt tttttgtaat
     4801 ctaagtgatc gttcaaagga atcaatgcga tgatcacccc agttttgttt cctttcacta
     4861 cgagagtttt acatatcaaa gctggatcaa tccctctttt ttgtagttct tctgtgaaag
     4921 attcttctac cacatgacta aaatcaagtg gttgatagct gatcttttgt tcttctaaat
     4981 aacgttcgac ttttgtttga ctcatcattg attcactccc tttattctat cgtttattat
     5041 aaagcgaggg atgctgtatt caaaaattat ttgtttttcg caaaaaaaga gcaagacgag
     5101 cactctatag agaggttcat cttactcttt ttgtttattt tttatttcga cgttgagtag
     5161 cttccataat gactggcaag atcactgggc ggcgttttgt ttgttcaaat aagtaacggc
     5221 ctaattgatc tcggatgtct tgctttaatt tgctccactc aaaatcattg ctttctaaat
     5281 gtttctcaac gattgtttca atcatattgc tgctttcttt catcagatca cggctagctt
     5341 ttacgtaaac gaatccccgt gaagtaattt gaggttttga aatgattttc ttttctcgcc
     5401 gactgatagt gacaaccgct acgaaaatcc cgtcttctga caagattttt cgatcacgta
     5461 aaacaatgtt tccgatatca cctatgccta atccatcaat cattacattt tctgcacttg
     5521 tagttcctgc aacactcata cgcttgttct tatattccag cacgtctccg cgtccagtaa
     5581 tatagatatt tttgtatggc atccctactg catgagccaa atctgcatga gcagctaatt
     5641 cacgatactc tccttgaact ggcacaaaat atgttggttt catcaaattg atcattaatt
     5701 gcaagtcatt aggattggca tggccagaaa cgcgcatatt ctcagagatc aacttcactg
     5761 ttccaccagc tcgataaatg atatcttccg ttttcgccac gatggtttcc atcgcgatgc
     5821 ttggcgtagt agtgatataa accaaatctc cttcttcaat cttgacatta cggtgtgtac
     5881 cgtttgccat tttttgcaga gacttgatgg gttcacccat tcggccagtt tctaaaatga
     5941 tcagttcttc tggatcatag tttttcatgt ctttcaacgt aacaagcaga tcttcacttg
     6001 gtaattgaag tttttctaaa cgcatcgctg tgcgaataat tcgttcgaaa tcttgaccag
     6061 tcaagacaac ttttcgttgt gctttttctg ctgcatccaa tacttgttgc actcgttgca
     6121 aattacttgc tacacaagcg acgatgatac gcccttccca atatttgatc gtatcaaata
     6181 cttcatctgc tatttgggct tcagaagcta caggctgagg attttctgca tttgccgatt
     6241 cacttagtaa ggctaagact ccttctttgc cgatttccgc caatcgtgca taatctgttt
     6301 gatacattgg gatagccgtt tggtcaaatt taaaatctcc cgtataaacg atactacctt
     6361 cttttgtttt cagattgatc cccactgaat ctggtatcgt atgagtggta cggaaaaagc
     6421 tcactactgt ttcaccaaag tctatttctg tatgctcatc taccacatgg aaatccttaa
     6481 atcctttggc tgcttcatta cgactgacat tcaattttgc taactcgatc gtcaactctg
     6541 tcccgaatac tggtacagaa atttttgaca acaagtaagg aagtgccccg atcgcatctg
     6601 catgtccatg tgttaaaaag acaccagcta cacgatcaat gttttcttct aaatacgtaa
     6661 aatcagggat tacgacgtcg atccccaaca actcattttc aggatatttt agcccacaat
     6721 ctaaaataaa aatatcctcg cccacttctg cgacatacat gttttttcca ttttcacgaa
     6781 cgccgcctaa tgggataatt ttaatggtac tcacaaactt cacctcttct tatgacatcc
     6841 aagtttaaag atgtcgattt tcttctttat ctaaatatat gagccattcc aatgaagcat
     6901 atgcaaaatc actatttctc aacttatgga gaatcagcag gaaaattacc agcacatccg
     6961 cgttctgaca gttggatggg cattttattt tggatatact tcattaacaa aatggatctt
     7021 ttgcgaaacg ctatagccag aatacttata taagtattca gctgttcccc attattatac
     7081 aacaaataaa ctaaatcgct agtatcagca attgttagaa caactatgca cgtaaaaatc
     7141 cctctgtagc atctaggatt tcttttgcta ttcctctttc atttcttctt cgtcggtttg
     7201 aaacatactt ttttcatttt accacatttt ttgcaataac tatagtgact ttttatatat
     7261 acagaacctc atgttgatgg tgtgcaaaca gcataagatt taagctagct ttaagatagg
     7321 ttgtctatta tcaaatgcat ctactaggag ggatgcaaaa tatgaatttt atgaaacgtg
     7381 catggaaaag tacaaaagta aaatggggac gttcattgtt attgtttgcc gtgttcacag
     7441 ccattcttat ttttgtgcta gctggcctca cgattcgaag cgctgcagaa gaagcagcga
     7501 atcaagcaca aaaagaagtt ggagcaacag tgactttaag tgccaatcga gaagcttcat
     7561 ttaaaaaaca ggaacaaact tcttcaagcg gttctaccac tgaagaacgt ccagatccag
     7621 gcagtttttc tcttacacca gtttccttaa cggacgcgga aaaaatagct gaattagata
     7681 atgtaaagag ttattccttt gaaagttccg cctcagctat ggcaaaagat ggaataactg
     7741 ccatcactag ttctgattca agtgaagaca gttcagaaac tactgatgca ggcggtgaac
     7801 ctgctggagg aatgggcggg aatccgcaaa tgactcaagc agactttcaa atcagtggtg
     7861 tgtcagcaac tgcacaaaat agtaattttt ctgatggaac agcaaaaatc acggatggtg
     7921 aaggaataga tgacagcgac aaaggcacaa acaatgtagt cattgattct actctagcag
     7981 aaacaaatga cctggcagtc ggagatactt tcacaatcac gagtactgaa gatgaagata
     8041 ccacttatga aatgacgatc aaaggaattt atgaatccag cgaaacaagc ggcagtatgg
     8101 gtatgaaatt caacttcatg aatccgtcga atacacttta cacttcttac acttttgcaa
     8161 atgaactaaa tgggacatct gacgataaca cgatagattc tgctatttac actctatctg
     8221 atccagataa aatggatgag tttctcaaag aagctgagaa gctgatcgac acagatacat
     8281 ttagcttaca atcaaatgat tccatgtatc agtcaatgtt ggaaccttta aacaacgtgg
     8341 caagtttttc taaaaatgtt gttttactag tggctgtagc cggtattatc atacttactc
     8401 tgatcattat gattaccatt cgggagcgtc ggcatgagtt aggcgtcttg ctatctttag
     8461 gagaatccag aagtaaaatc attctccaat tgtttaccga agtggctatt tgtatgattc
     8521 ttgcattagg agttgcttcg cttagcggaa acgtcgtcgc gaatgccgtg ggacagcagc
     8581 tacttgacca acagacagaa accacgacac aaaaccagca agctcctggc ggtggacaaa
     8641 tgccaggtaa cagacaagaa caaggcggtc aacgcgatgg tcaacaagga accacgaata
     8701 atccatttgc tgtttctgat caagtcagcg aattggaaat caatgtcaaa cctgcacaat
     8761 tgggactatt agccggagta gcatttggaa tcagtctgtt ttctgtcttt ttagcttcta
     8821 tagggatatt aagattaaac ccaagaaaaa tcctattaaa ctaaaggagg aatttgatgt
     8881 tacggacaga acaattggga ttttggtatc aagatccgca agatgcgctt tataaagatg
     8941 tagaactgaa tttcgaagct ggaaaaatgt acgctatttt aggagcaagt ggttctggaa
     9001 aaacgacttt tctttcgctt atttctggac tagatgcacc aaaagaagga aaggtatact
     9061 atcaagatcg ttcactgaac aaaatcgggt taagaagtta tcgtaaaaat gatgtctcga
     9121 ttattttcca agcttacaac ttgcttcctt atttatcggc tctagacaac gtcatagcag
     9181 caatggaaat cactaagacc aagcaagaga attatcgaca agttgcttta gatcgactgg
     9241 ctcgtgtagg aattgatgaa atattagcaa aaaaacaggt gaccaaactt tcgggaggtc
     9301 agcagcagcg agtcgctatc gttcgtgcta tctgctgtga acatcaacta atcgtagcgg
     9361 atgagcctac tggaaatcta gatgaaacaa cttcacaaga tatcgtcaaa cttttccaag
     9421 aaatcgccca tgaacaaaat cgctgtatta tcatcgtgac tcatgagatt gaagtagcaa
     9481 aaagctgtga tgaagtcttt gaactaaaaa ataaaaaatt tcaaaaaatc gaagtttaaa
     9541 aaacaggata atgatcaaat aaaaaatccg aaccatatct gtctcgcaga tatggttcgg
     9601 attttttatt tgcttgattc aattagtaat aagtggtttt tatttttttt cgtcttttcg
     9661 agaaaacttg tctttgactt cgtcgcctaa atttcgagct ttttctttga tgtcagcacc
     9721 tagtgattgt gctttccctt ctgtttcttt gctcttgtcg tctgttgctt tgccgtatgc
     9781 ttcttttcct tcaccgacta ctttatcttt catatcttta gaacgtcctt ttaaatctgc
     9841 cattgtgctt cctcctctta gtttgtttat tgatctacct tcagggtaac atcaacaaca
     9901 gatacagaca aacagaacgc tcgttttcta aaaaagatag ttagagctgt tgattaaaaa
     9961 cttttttcga aagtccccca tactttgact tctttttctt ggaccattat ttctccttta
    10021 gcgataactg tatcaatatc aagcgtttct ttatccaaga tacacagatc tgcatcagca
    10081 ccaatttcga tttttccttt tttatccaat ttcaatatac gtgctggatt actggttata
    10141 gctcgtaagg cgatttccaa agggatactt tctttttgca cagcttcttt gatccctact
    10201 agcaatgcct tggcacttcc tactcctaac cctaggaaat gattgttttc atcaaaataa
    10261 ggcaagcttc cttgtccatc tgaggtcatc gtgaaattat ctaaagaaac atcttcctcg
    10321 atcaatcgtt tcagtgcttt gctgaagcga acttcaccat ctgtcttctc ccaaaaatca
    10381 ggatcttcgc tagcagtgaa gtcaatcgtg ccaccacgtt tggcaaatgc cacgcaagct
    10441 tcaaataatt ctggtgtgcg atttgcatga gtaggcaaaa attgtgtgat cggaatttct
    10501 gtttcttcta cgattcgtgt caatagctct agctttcttt ttcctccgcc taagtggaca
    10561 ttgatgatcc cagcttttcc ggataacatg cctcctacac gagcatctgc tactgcacga
    10621 gcgaactctt caaaacttgg ctgagaagaa cggtgatcgg atactgcgat ttctccgatt
    10681 ccgataattt tatcaattgt caaaaagtct ttgatgatag aatctgttac tgtttttacc
    10741 ggcaatcggt acgaaccttc gtatacataa gtggagattc cttctgcttc cagacctttc
    10801 gctttgctga tcaacgcagt catatctcta ccttctccgt ctgttcccaa acaaccgaca
    10861 actgtcgtta caccagctgt agtcaattgg ctcaatgtga cttctggcgt tcgattttga
    10921 taaccgcctt ctcctcctcc acctaaaata tggaaatgac agtcaatgaa acccggggtt
    10981 agtacttttc cctcacctga aagttcttct acttccactc cttcgtagcc gccttctagc
    11041 tgttcttcta cagccaaaat cttcccaccg gcaatcagta catccatttt tcctaagtaa
    11101 tctggtgcaa acacttcaat ttgtcgaatg attttcatac atttttcctc tttctttaag
    11161 tataactgat gataggatct taaaaaataa tccaaacgat aggatcttta ctcctttgat
    11221 tttaccgtaa aatgacggtt cttggagtcc ttatctactg tttggattac ttttttattt
    11281 acaggtctcc agtactactg cagacctatt gcaatggcaa tcatgatgaa aatagttcct
    11341 aaaacaaaca ggaatccttg catcttgatt tggaatcttg cccattttgc ccattcgatc
    11401 cgtgcaacac ctagcacacc aatcaagctg gctgatacgg gtgtaaaagc gtccataaaa
    11461 cctgaaccaa gctgataagc aagaactgct acttgtctgg atacacctac taaatctgcc
    11521 agtggagcca tgattggcat agtcaaagct gcttgtcctg aatttgatgt aacaaccaag
    11581 ttgaacaaac tttggaagat gtacatcgcc caagcaccaa tgacagctgg aacaccggtc
    11641 aacgccgtcc cgatcccatg caaaatcgta ttcaaagctg aaggaacaga tgcatcagat
    11701 cctcccaaga caagaagaat ccctttggcc atacctacta cgattgctgt accagctaaa
    11761 tcagctgcac cagattggaa agaagaagcg atgtcattga ctttcattcc atctaatttg
    11821 aaaagaacag caatgattcc agcaactaat cccatcacaa agaattgaga agcgatttct
    11881 ggaatgtaat agcctttttg agtcacgccc caaacgatcc agacaaggac aactaacatc
    11941 tctaataaga tcaatttatg cccaagcatg aattttttat tttcatcaga tgtctttttg
    12001 atatgagaac ggaaataatc atctgatttg taagtcaacg aactttctgg ttttttacgg
    12061 attttttctg catatatcat catataacca gcggctaatg cagtaacaac tacccacata
    12121 atcaatcgga atgtcgcacc agacaacacg gggattccag caatcccttg agccaccgct
    12181 acgctaaacg ggctcatcca agaggtcgca tttccgactt gagatgcgac ataagtcacg
    12241 gttactgcta cgatcgagtc atatcctaac gcaatgacaa atggaaccat caccattgag
    12301 aatggaatta cctcttctgc catcccgaat gtagcgccac cgaatgaaaa agcaaagaat
    12361 aatagcggta aagctaaacg ctccagtccc ttagttttac tgataaacgc atagatacct
    12421 gcgtcgatcg ctccggtacg cataataata ccaaaagcac ctccaacaac aaggatcaat
    12481 gcagcgatcc ctacagcaga tccatactta tctccagata ctagtccttc aaaaacaaaa
    12541 tttaagaaac caaagccgcc aaaatcatct gttccccata ttttggctgt tttatgcaat
    12601 tttttagaag tatcatagat attttcgccg tactgatcgt aaagcacatc atctgtcaag
    12661 gagatttcat ctagtttttc ctgcgtaaga tttttttctc cgtcagcaat gactttttcc
    12721 aatccttttt ccggaacatc cagtttttca cgttctgctg gatgatcttg cagttcttct
    12781 aattgatcaa acacatacga cttatccagc tcgtaagcat aacggaagga atcttgacgt
    12841 aaaacagtac gagtacttgt ttcgccattt gcatctttgt attcaatatc ttgcgtgctg
    12901 aactttcctg ctggaacgac aaaagtcagt atccatgcaa agatgactac accaaaaata
    12961 atgacatacg tatggggtgt cgataattta gtgaagtcgc gtttcggttt tcctgaagaa
    13021 tttgttttct tcaaatttat ttcttccttt cttttttcag taattttgat gaataaaaaa
    13081 tggataatca aatgaattat acagtaaaaa tgacatttcc acatgaataa tttattagaa
    13141 aatcactttt ttctattttg ttttttttct cctatgtatg attatttttt tctctttcca
    13201 tttatattaa gtattttatt tctctgtttt tccttaaaat tcttttttta tttttagatt
    13261 agagaaaatc tcgtcctgtt tttttcattt tacaatcatt gaaaaattga atttcattgc
    13321 aaaaaaaata ataaacactt ataaatatcc attatctatc cttcaatttt tgtgtatttt
    13381 atttctatta gaaaaaggta tcctgttgga caattttgtt atatcaggta aacttcattg
    13441 caataatgat aacaaatgta ctataattac tttgtaaaga gttttgggga agactctttc
    13501 atcaacgtgt attaagagac aagaaagaat ccatatggat tctttcttgt ctctttttcc
    13561 actacttttt atttttctga cactacatat tcttataact catttctcat aactgtgaaa
    13621 caacttataa aaataacaaa ctttaaacaa aacaagctta aaatcaaaga tgatccatct
    13681 taaaataact aaaacaaaaa aagtagtcgt tgttaatcct cattaataat cgttttcttt
    13741 ttttcaaacg ctccattttt atgtggaaca ttattttttt aaggaacttt gtcaacatgt
    13801 ggataactat tttccacttg tgtcctggac tagctgtttt gctgtaaaat acaaaacttg
    13861 ctgcagcaaa aagatcgttg caagagaggc tgttccgcag atcgctggat ctagatttgg
    13921 caaagtcaag actgcatcat gtaaattggc gtggttgtcc aaaaccaaat cagcaaattc
    13981 aaataatcgc aaaccactgc tatgttgcga tttgatactt cgtgacaaat caaatccagt
    14041 aacaatgatt aatggatgac cattttcttt gatccactga gctagttcga tgattgccgg
    14101 gtttcttcct tcattcgaaa gaagaagaaa aatgtcttct gatttgacag aaagctgatc
    14161 aataattgcc tgtcctgcac cttctagcgt ttcatagaca cctccggctg gattttcaat
    14221 caatatactt ggtacaagat ccggggtttg atcccaaaat tcaaatgcta caccacttag
    14281 tgtgcggcta gcatatatct gcaatctacc accttgatta atttgtttcg cgatccaagt
    14341 gccagcttga agcatcagcg gctcttctat ccttaatttc tcttccaatt ggtgataata
    14401 gtcaaaataa cataaactca tttatctctc ccgcctagct atacttgaca tgaatagaaa
    14461 cagctgccta atctaaggaa gtacataaaa aaacagtagt tagacagtaa cacatgtggt
    14521 ctttgctgtt tcaaacacaa aaaaggcatt gaacgaatac gcccaatacc ttgacatagc
    14581 aaataaaaca acgtgcagac catggaaaat atctacatag gggtagaatt ttcaccctag
    14641 attaggtaca ataaattagg tcgatgcact gcattgacta tggcaaaatg atcggtcagg
    14701 gtgtatgtgt gtgactggtc attccctgat tcttagcgcc aactaagggt cagggagcct
    14761 ttttttattc agctgtttct atctgaattt attgtaagta atctcttatc caagtgcaag
    14821 caagaaaata caaaaaaaca ttggtatgat ttatatatat ataaataaca taaaaaaagg
    14881 aatggcttat cccgatacaa caagcattcc tttaaaaaag attcttttat ataagtgaaa
    14941 aaagcttttt tccaccaatt aaataaatct ctcatctcaa tagtttatcc attattttat
    15001 ctttttttgc gtatacctac taaatattac cgtcttcttc tcgttcttct actttgttcg
    15061 agagaatctg tactagaaaa agtagttgta tgtctacctt ttctgcctat ccgagacttt
    15121 cttttcatat atgggatata aatgattaga tacagtacag caaaaaataa agcaaatacg
    15181 caagcttccc aactacctaa cgccaattca accatcaagg tagcaacccc aacggatgct
    15241 gcataccaga taaaaatcag aaacgtttct ttttttctga ccatgcgaaa catgataaat
    15301 agactaataa gcaatagtgg caacacaaat atccaattgc ttccttccat cagccacttg
    15361 agtatatcaa gcagcgactg tgcaacagcg actaattttc ccatagtgaa cacacacctt
    15421 tttttataat taaaaaattc gcctttttat tctactatga atcttctggc ttggctttca
    15481 tttctcttat gtaatcattt tcttttatac aacttaaaaa aaaattaaac cattagcata
    15541 atattttctg aatctattca cgaaagtctt tttctatctt tcgttttcca cgactcaact
    15601 gatctagagt caaaaaacta aaacgacagt tgcgcaaatg acgcacaagc tgccgcttta
    15661 ataacaaata gcctttcatt ctcagcaggc cacttatttc cagttaactt tctgttctta
    15721 gctttttact caactggata atatatgcac ttaatgaagt cccaatttcc gaatgttgaa
    15781 gtccatattc aatatttgtc ttcatatatc ctaacttatc gcctacgtcg tatctttttc
    15841 ctttaaactc tctcgcatag accgcctctg tctggttaag agtctgaagt gcatccgtta
    15901 gctgtatctc gtttcctgct cctggttctt gagtttctaa tatctcaaat attttaggag
    15961 tcagtagata acgtccgata atggctaaat tacttggtgc ttttcccatt tcaggttttt
    16021 ctacaaaccc ttttactctg caacgaccct tttcgctatc tgttttagga tcaataattc
    16081 cgtatttaga tgtttcattt tctggtacac gcatgacggc aacagcggaa gcttgtgttt
    16141 cttcataatc catcatcaat tgttgagtca atgggatatc atctttcatc aaatcatccc
    16201 ccagcatgac gacaaacggt tcatttccta taaatgcttt tgcttgaagc acggcatccc
    16261 ccagtcctct tggatgagat tgacggatga agtgaagatt gacttgagtc gtttcttcaa
    16321 ctaaagcgag taattctgtt ttttcctttt cccgcaaatt gttttccaat tcgatatttg
    16381 catcaaagtg atcttcaatg ggtctttttt cttttcccgt tacgatcaag atgtcttcga
    16441 ttcccgaatc caatgcttct tccacaataa actggatcgt tggtttatcc acgattggaa
    16501 gcatttcttt tgccatggct tttgttgccg gcaaaaaacg tgttcctagc ccagcggcag
    16561 gaatgactgc ttttttgacc ttcactt
//
LOCUS       DACEEZ010000063        16488 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20313_2017
            SAMN12639966-rid9879223.denovo.063, whole genome shotgun sequence.
ACCESSION   DACEEZ010000063 DACEEZ010000000
VERSION     DACEEZ010000063.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639966
            Sequence Read Archive: SRR10023158
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 16488)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16488)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741683.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741683.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:44:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,016
            CDSs (total)                      :: 2,962
            Genes (coding)                    :: 2,889
            CDSs (with protein)               :: 2,889
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 36 of 73
            Pseudo Genes (incomplete)         :: 29 of 73
            Pseudo Genes (internal stop)      :: 18 of 73
            Pseudo Genes (multiple problems)  :: 8 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16488
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639966-rid9879223.denovo.063"
                     /isolate="E20313_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            61..1182
                     /locus_tag="IWO89_002030"
     CDS             61..1182
                     /locus_tag="IWO89_002030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HAQ0456757.1"
                     /translation="MDILAAIDSFKGSASSFELNQAVLNGWEDRKGRKINVPIADGGE
                     NTAEAIYYALRGTWRTIDGMDLLFRQRKISYLLTTWQGKNTAVIESAQALGLDLLDKP
                     TDQTIRLASSYALGELIKDALLQEVEQIIVTLGGSGCSDGGLGLLQSLGASLEGYTEG
                     NPLLSTKKLHFDRRNVLVSKVKLIIAADVTNPYTGQQGAAWQFGRQKGGIDTTLAFLD
                     KQAGKIAQQIKDFWEVDLDDIPGSGAAGGIGGALFLLGAKMVSGFDLVSQITGLEKKI
                     EQADVIITGEGRIDQQTIHGKVPYGVAVLAKKYRKPIIAICGSRDQELEGLSDFLPIV
                     LSIHLGPIGLEEAMEHRQTLEKTKILGQTLSRFFTILPK"
     gene            1440..2942
                     /locus_tag="IWO89_002031"
     CDS             1440..2942
                     /locus_tag="IWO89_002031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F type ribosomal protection protein Eat(A)"
                     /protein_id="HAQ0456758.1"
                     /translation="MSKIEIKNLTFGYDSQGTLLFEQANLNFDTQWKLGLIGRNGRGK
                     TTLLNILQNKLPYQGQVIHQQEFAYFPQQTKDKERLTYYVLNDITDFEIWEIERELQL
                     MQTDPEILWREFSTLSGGEKTKVLLALLFVDDTHFPLIDEPTNHLDISGRKQVAAYLK
                     KKKQGFIVVSHDRGFIDEVVDHVLAIEKSQLELYQGNFSIYEEQKKLRDEFEMAQNEK
                     LKKEVSRLKKTAAEKAEWSRSREGDKTKKQVGFIDTESRRVNKGAVGADAARTMKRSK
                     AIVNRMETQISEKEKLLKDIEYIDSLTMNSQASHHKRLLSVEDLQLGYENLLFEPIHF
                     TIEPHQRVAISGPNGAGKSSIIHYLLGAFNGKVIGEKYQPKHLSISYASQNYEDNRGT
                     LAEFAEKNQVDYQAFLNNLRKLGMERDVFHNKIEQMSMGQRKKVELAKSLSQPAELYT
                     WDEPLNYLDVFNQEQLEQLILNVKPAMLLVEHDQTFLDKVSTEIISLERI"
     gene            3009..3536
                     /locus_tag="IWO89_002032"
     CDS             3009..3536
                     /locus_tag="IWO89_002032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HAQ0456759.1"
                     /translation="MLYVTRHGETTWNAQGLVCGRADVPLTEKGQMQAQKLAEKVVDL
                     PVPITKIIHSPLQRARDTAQAVADRLSLPLTVDERLVEMDFGDYDGLPSKDENFQKAR
                     LAFAVRFPNGESVLDVYARIVPLLKECIEDEENVYLLVCHNALIRVINAYFHPMPNEG
                     FFTFMVDNTELVSYE"
     gene            3747..4808
                     /locus_tag="IWO89_002033"
     CDS             3747..4808
                     /locus_tag="IWO89_002033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF871 domain-containing protein"
                     /protein_id="HAQ0456760.1"
                     /translation="MFGFSIFMNDSLSEEKKQYIRQMASNGFVGIFTSMHIPEDDVSA
                     YQKRLIELGSEAKKNKLELMVDISGEALERAGFSLEDLTPLKAIGVTGLRMDYHISNQ
                     QIADWSHQLKISLNASTITPKDIDELKEAEADFSQIEAWHNYYPRPETGLDKEWYQKK
                     NQWLKIQGLLVQGFVPGDTELRGPLYQGLPTLEEHRGIHPLAGALDLLASNTDIVYIG
                     DAGLSENVQEQFASFQKEQTVLLHTEPVDEEFYEYILGKHTNRQDDARDVIRSVDARF
                     REIPPIPARNTATRMKGSITLDNEKYLRYMGEIQLTKYDLPADEKVNVVAKVIKEELP
                     LINQIKAGMNYQFIRKEGR"
     gene            4811..5707
                     /gene="murQ"
                     /locus_tag="IWO89_002034"
     CDS             4811..5707
                     /gene="murQ"
                     /locus_tag="IWO89_002034"
                     /EC_number="4.2.1.126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramic acid 6-phosphate etherase"
                     /protein_id="HAQ0456761.1"
                     /translation="MSKINLENLTTERRNQETFGLDEMSISQAVQLMNKEDQKVAAAV
                     EKELTSIEPVIANTIESFKKGGRLIYMGAGTSGRLGVLDAAECVPTFGVEPEMVIGLI
                     AGGEEAMTVAVEGAEDSYELGQEDLIKLNLTKNDIVIGIAASGRTPYVIGGLDYAKSI
                     GAVTGTISCNKQAEISKHADFPIEVDAGPEFLTGSTRLKSGTAQKLILNMISTISMIG
                     IGKVFNNLMVDVKPTNEKLIERSKRIIMQATEADYETAEKYFEEADQNVKLAIVMILT
                     NSDKEEAQAKLAEANGFVKGTL"
     gene            5721..7703
                     /locus_tag="IWO89_002035"
     CDS             5721..7703
                     /locus_tag="IWO89_002035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAQ0456762.1"
                     /translation="MAKNELTLEELAKQIYKGAGGMGNVESIVHCMTRVRMKIINDDL
                     VNVSELKAIPGVLGVVEDEQLQVIIGPGKVNKVAQQMVDLAGVKLGEKLSSVSTATAN
                     SSASGKDRVNERAQEMKAAQKSKQKPSKIKSVLKDISNIFVPMIPAFVGTGIVAGIAA
                     VLANLVTAGTLDAATWQQYIDVMNILKNGMFSYLVIYTGINAAQVFGATPTLGGVIGA
                     VVMLTGMNPEAPLKNLFTGEALAAGQGGILGVIFAVWLLSIVEKKLHKVVPDAVDIIV
                     TPTLSLIAIGLIEIFFIMPLAGFISDGMVGGINWVLGVGGAFAGFVLGTLFLPMVMFG
                     LHQILTPIHVQMIDETGRTLLLPILAMAGAGQVGAALALWIRCKKDKELTEMIKGALP
                     VGILGIGEPLIYGVTLPLGRPFITACIGGGIGGAVIGMIGNVGAIAIGPSGAALIPLI
                     SDGKWLGYILGLLAAYAGGFVATFFFGIPKDRLEKEELATETATVNEVPEKVVPQTSA
                     ESVTILSSVADGTVVSLDKASDPVFAQKMMGEGYFVEPENGHIYSPVTGVVSSVFPTK
                     HAIGITTPNGLEILLHMGINTVDLGGTPFDVKVTEGQQVTPDTLVAEADLEAIKAAGK
                     ETSMIVLVTNMDRVKNFVLEKTGKAKAKAPVMDVKA"
     gene            complement(7768..8214)
                     /gene="nrdI"
                     /locus_tag="IWO89_002036"
     CDS             complement(7768..8214)
                     /gene="nrdI"
                     /locus_tag="IWO89_002036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class Ib ribonucleoside-diphosphate reductase
                     assembly flavoprotein NrdI"
                     /protein_id="HAQ0456763.1"
                     /translation="MNILYISISGNTRAFAKHLAEYAEKMHAEDPVNPEVTLKEIHEN
                     SDFTKETEPFFTFVPTYLDGGNGLDNGDTEILTETMREYLEHEDNHKLCLGVVGSGNK
                     NFNNQYCLTAKQYAQTFGFPFLADYELRGTPSDVTRIYQILAENNK"
     gene            8425..9849
                     /locus_tag="IWO89_002037"
     CDS             8425..9849
                     /locus_tag="IWO89_002037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAQ0456764.1"
                     /translation="MDTEQQKKGIGLIPLAALVIGSAIGGGVFGIMTDISGSAGGPAI
                     FSWVLVGTAMLMLSLSINNINKKRPDLKGGIFSYAEEGFGRFAGFISGWGYWLTCWLG
                     NVAFATLLMSALGYFFPVFEGGQNLASIVMSTVFLWGYAWLVNRGVENASFVNAIVTF
                     CKLVPLFLFIVVAISTFSIQQFIDNFSSDIILNANGQPIPFSSQVLSCIMVMLWVFVG
                     IEGASVVGSRAKKQSDVGKATILGIFGLIIIYVLVSMLPYGTMTMNQLQTIKAQPAMG
                     YVFEMMVGKWGAVVINGGLIISLVGVWLSWTILPIETMGNMAEDGLLPKAWGKLNKNG
                     APTYAIVLTTLCTNVFLLSLLFTDSAYNFAYTLGTAAIFFTWLFLGLYQMKLSYQRKE
                     WVQFLVGLLASAFQVWAMLVVAFNEVMLVLVLFLPGIYFYLKARKEQNEPVQHIHLDK
                     ILLIGVTMVGVIAMVLFATGMLHV"
     gene            complement(10030..10803)
                     /locus_tag="IWO89_002038"
     CDS             complement(10030..10803)
                     /locus_tag="IWO89_002038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HAQ0456765.1"
                     /translation="MTLLVKQELFKLIKKKSTAVLSVLLVVLLIGTALLAKKYTTIID
                     PVEMTAQLFSATSWIVFIMIAAASTIISMEAQYGTLKNLLYRKYSRGEILVSKWITLV
                     IYSVYLYLLAIIVTVLMKLILFPSISFTEKVSTGQTLIQSLFTYTLGSYIGLWLILSL
                     VLMIACFINSSGASISAGIVFYFASSIISGILIALIQKWEWIKWNPISMLNLQNQIGN
                     EEIMKQLTHLSTNQMLFGNLAYIVLFLALGYLVFKRKNV"
     gene            complement(10800..11705)
                     /locus_tag="IWO89_002039"
     CDS             complement(10800..11705)
                     /locus_tag="IWO89_002039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAQ0456766.1"
                     /translation="MKSLSVQELSKNFGHKQALSDVSFSLSPGKIVGLVGPNGAGKTT
                     IMKAILGLIHYSDGTICIGENEISPSSHKGLEQVGALIEYPGIYPFLTGYDHLKLFSE
                     TNDVSAIDTIVNQLKMEKYIHKKAKSYSLGMKQKLGIALALLNHPSIVILDEPMNGLD
                     PQATRDVRKIITDLATQGTSFLISSHLLSELEKMVDELILINQGKIIKQCTMAELNQT
                     AQDFIVISTTNDLAARTILTEAGYTLEDPDKIKLLKENEELLGTVIQLLSAQNIHVTD
                     VQHLTNDLESSLLEILDSVKGEEEK"
     gene            complement(11859..12179)
                     /locus_tag="IWO89_002040"
     CDS             complement(11859..12179)
                     /locus_tag="IWO89_002040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux SMR transporter"
                     /protein_id="HAQ0456767.1"
                     /translation="MAWMELIIAGILEVFWSTMMKWSDGFSKINYSFYTVIGMIASFY
                     FLSKAIKSLPMSLSYPIWTGIGAVGSVIIGVVLFHDKLNLSTWFFVGMLLISIIGIKI
                     TSGH"
     gene            12653..13339
                     /locus_tag="IWO89_002041"
     CDS             12653..13339
                     /locus_tag="IWO89_002041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF969 domain-containing protein"
                     /protein_id="HAQ0456768.1"
                     /translation="MENYLSLLGVLIVILGFAFKLDSILIVMVALVVTALTGGLGIQG
                     MLELLGTSFVNNRGMAIFIIIMLATGTLERNGLKESAASLIKRFKKVSAGMIVDIYGI
                     FRMIFAAFNVSFGGVAGFVRPIILPMALGTIESKGLEVHPKHEEELKGMASAMENVCW
                     FFGQVLFIGGAGALLVQSTLKDLGYDVTLGQLALVEVPVAITALVVASVYFYLKEKKL
                     AKKYYPGKGQ"
     gene            13341..14285
                     /locus_tag="IWO89_002042"
     CDS             13341..14285
                     /locus_tag="IWO89_002042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF979 domain-containing protein"
                     /protein_id="HAQ0456769.1"
                     /translation="MDFFTNPDILLGDKLLEILYIVMGLVLIYTGVRNLTDKTNPHRY
                     GSAFFWTVLGVVIAGGRWLPAIVNGGLIFAMTIPAIAKKVSKGESRLPSKEYMEKMSD
                     KLGMKIFIPALSIGVFAILFALFTNLGALVGVGVGVFAAMLILMFFSRDNRPSTFLDD
                     AADMLGTVGPLSMLPMLLASLGAVFTSAGVGTVISNAVGTIVPEGNVNIGIIIYALGM
                     VIFTVIMGNAFAAITVMTVGIGAPFVFSHGANPALIGMVALTCGFCGTLLTPMAANFN
                     IVPVAMLEMKDKYGVIKNQLFIALFMLVFQIAYMILFK"
     gene            14484..15128
                     /gene="pcp"
                     /locus_tag="IWO89_002043"
     CDS             14484..15128
                     /gene="pcp"
                     /locus_tag="IWO89_002043"
                     /EC_number="3.4.19.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyroglutamyl-peptidase I"
                     /protein_id="HAQ0456770.1"
                     /translation="MKVLVTGFDPFGGDKVNPAYEAVKKMPDEISGAEIIKVEVPTVF
                     EKSSQVVKEAIQQHQPDVVICVGQAGGRSAVSFERVAINLAEARIPDNEGNQPFDTAL
                     EENGPAAYFTSLPIKAMTKNVQDHGLPAYISYTAGTFVCNDIMYRLLHLIETQFPAIR
                     GGFIHVPFSPDQVIDRPVGTPSMSLEDIASSLTYAVEAAVENKEDISGNAGTTH"
     gene            15207..16484
                     /locus_tag="IWO89_002044"
     CDS             15207..16484
                     /locus_tag="IWO89_002044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0456771.1"
                     /translation="MEEWQSVFEEWFPKEISKSYPIKISKQYTSSQRWEIYAKLTKKQ
                     RELVDKHRRYLISSRFMEEHYLAATDWVFSDFKINPFFRTKRSQQKLYCECGRELKVQ
                     YIVKSPKTGKILKLGINHFADHLHVSPTVAASIHQGMTKVDLALDELLWLKQKNIDFP
                     EGLWQKYCFVLYQNRRMKQPYLPDIKLAQRLAEFRQVEMPIYIADYQALENEIKKISE
                     HINGQSKKRQIKKELFDDFAEELVKDVEEFLINYRAFLRKDWQSIVYEEVPVHPNAYF
                     ETFISVLRKTKRQRTPEVTAQMEYFAKNQRFIQPKIYLFIWKQYCRYGFTEGFFDSIP
                     RIVRNGFLKVLRKEREAIQSADKKDRTVSKEKWQLVVKDIQSGNVQETIDKWKGKHYR
                     FTEAQKQALEYYQKLEESLRFNDEARKYLKELL"
BASE COUNT         5314 a         2893 c         3564 g         4717 t
ORIGIN      
        1 tgttttgttt gaaaaaaatg aaaaaaagtg tcttacttaa gaagaaagtg agggatacag
       61 atggatattt tggcggcaat tgattcattt aaaggaagtg cttcttcatt tgaactaaat
      121 caggcagttc taaatggctg ggaagatcgt aaaggaagaa aaatcaatgt gccgattgca
      181 gatggcggag aaaatactgc agaagcaatc tattacgcgc tcaggggaac atggcgtacc
      241 attgatggga tggatctctt attccgtcag cgaaaaatca gctatttatt gacaacttgg
      301 caaggaaaaa acaccgcagt catcgaatct gcacaagcat tggggttaga tctacttgat
      361 aaaccaacgg atcaaactat ccgacttgct tccagttatg ccttaggaga acttattaaa
      421 gatgcactgc ttcaagaagt agaacaaatt attgtcactt taggaggaag cgggtgtagc
      481 gatggtggtc taggattact ccaatccttg ggtgcttctt tagaagggta cacagaagga
      541 aatcctttat tatctactaa aaaacttcat tttgatcgaa gaaacgttct tgtttctaaa
      601 gtaaaattga ttatcgctgc agatgttacc aatccatata caggtcaaca aggagctgct
      661 tggcaatttg gaagacaaaa aggaggaata gatacgacgc tagctttctt agataaacaa
      721 gcaggaaaga ttgctcagca aataaaagat ttctgggagg tggatttaga tgatattcca
      781 ggaagtggag cagcaggcgg tattggtgga gccctttttt tgttgggagc gaagatggtt
      841 tctggtttcg atttagtcag ccaaatcact ggattggaaa aaaagatcga acaagcagat
      901 gtcatcatta caggagaagg gcggatcgat cagcaaacga tccacggaaa agttccatac
      961 ggtgtagcag tgcttgcaaa aaaatatcga aaaccaataa tcgctatttg cggaagtaga
     1021 gaccaagagc tagaaggact ttctgatttt ctcccaattg tcttaagtat tcatttgggt
     1081 ccaataggtt tagaagaagc aatggaacac aggcaaacat tagaaaaaac aaaaatctta
     1141 ggacagacac ttagcagatt tttcaccatc ctcccgaagt agttgacaaa atcattttct
     1201 ttatgctatc gtaatttcaa atataggaca aagtgaggga tccgtgcatt gaaaaattaa
     1261 gtcgattgat gaaagctgca ttcagactgt accttgtgga gacaaggaag cagtgtgcag
     1321 attttcggga ttggcttggt tttttcgatg tatgggtctt tttgtgtgta tccaaccctt
     1381 gcatcctctt tagccagttc tgaaaatagg actggtcttt ttttacgagg tgatggaaaa
     1441 tgtctaaaat cgaaataaaa aatctgacat tcggctacga cagccaaggc acattattat
     1501 ttgaacaagc aaatctaaat tttgacacac aatggaaact aggacttatc ggacgaaacg
     1561 gtcgaggaaa gacaacttta ctgaatattc tacaaaacaa actaccttat caagggcaag
     1621 taatccatca gcaagaattt gcctatttcc cgcaacagac aaaagataaa gaacgtttaa
     1681 cctattacgt gttaaatgat attacggatt ttgagatatg ggaaatcgaa agagagctcc
     1741 aattgatgca aacagatcct gaaatcttat ggagagaatt cagcacacta tcggggggag
     1801 agaagacaaa agtcctactg gcacttttat ttgtggatga cactcatttc ccgttaatcg
     1861 atgaaccaac gaatcatttg gatatctctg gtagaaaaca agtagcggct tatttgaaaa
     1921 agaaaaaaca aggcttcatc gtggtcagcc atgaccgggg atttatcgat gaagtagtgg
     1981 accatgtttt agcaatcgaa aaaagtcaac tggaacttta tcaagggaat ttctctatct
     2041 atgaagaaca gaaaaaactt cgtgatgaat ttgaaatggc tcaaaatgaa aaattgaaaa
     2101 aagaagtcag taggctaaag aaaacagcag ctgaaaaagc cgaatggtct cgttcccgag
     2161 aaggagataa aacaaagaaa caagtcggat tcatcgatac tgaatctaga cgagtgaata
     2221 aaggagcagt gggtgctgat gctgcacgga cgatgaaacg atccaaagca atcgtgaatc
     2281 ggatggagac ccagatcagc gagaaagaaa aactattaaa agatatcgaa tatatcgatt
     2341 ctttgacgat gaatagccaa gcgtctcacc ataagcgact tttaagcgta gaagatcttc
     2401 aattagggta tgaaaatctg ttattcgagc caattcattt tacaatcgag cctcatcagc
     2461 gggtggcgat ttcaggtcct aacggtgcag gaaagtcatc cattatccat tatcttctgg
     2521 gggcattcaa cggcaaggtt ataggagaaa aataccagcc aaaacatctg agcattagtt
     2581 atgcaagcca aaattatgaa gacaatcgag gaacgttggc ggaatttgca gagaaaaacc
     2641 aagtagacta ccaagcattt ttgaacaacc tccgaaagct tgggatggaa agagatgttt
     2701 ttcataacaa gatcgagcag atgagtatgg gccaacggaa aaaagtggaa ttggctaaat
     2761 ctttatcaca gccagctgaa ctatatacat gggatgaacc attgaattat ttggatgtct
     2821 tcaatcaaga acaattagaa caactgatct tgaacgtgaa acctgccatg ttactagtgg
     2881 aacatgatca aaccttcctg gataaagtat ctactgagat tatttctctt gagagaatct
     2941 aacccttgta atctcaagaa caacaaggta tggttaaggg gaactttgcc aacgaaggag
     3001 agaatcacat gctttatgta acgagacacg gagaaacaac ttggaacgca caaggattag
     3061 tttgcggacg ggcagatgtg ccgctgactg aaaaaggaca gatgcaggca cagaaactag
     3121 cagaaaaggt agtagatttg cctgttccaa tcacgaaaat catccattcc cctcttcaac
     3181 gagcaagaga tacagcacaa gcagttgctg atcgtctcag cttgccgctg accgttgatg
     3241 aacgattggt agaaatggat tttggggatt atgatggact gccaagcaaa gatgagaact
     3301 ttcaaaaagc acgtttagcg ttcgctgtac gattcccaaa tggggagtca gtcttagatg
     3361 tgtatgcacg gatcgttcct ttattgaaag aatgcatcga agacgaagaa aatgtctatc
     3421 tgttagtttg ccataatgcc ttgataagag tgatcaatgc ttattttcat ccgatgccta
     3481 atgaggggtt ctttacattc atggtagata atacagaatt ggtttcttat gagtaattat
     3541 gtttttgtgt tatttaaata aaatatttca aggcactgct tttgcttagc agtgcctttt
     3601 ttaggttatg acaattcttt tgataatata aactcaacct ttagaaatga aatttaatta
     3661 ttttaataaa agtattgaaa ttttgtttct ttagatatat actctagtca tagaaatgaa
     3721 gacggataca gaaaaagagg gataatatgt ttggcttttc aatatttatg aacgattctt
     3781 tgtcagaaga gaagaaacag tatatccgcc aaatggcatc caatggcttt gtcggtattt
     3841 tcacttccat gcatatacca gaagatgatg tttcggcata tcaaaagcga ttgatcgaac
     3901 taggaagcga agcgaaaaag aataaactag aattgatggt cgatatttca ggagaggcat
     3961 tggaacgagc gggtttttca ttagaagatc taacgccttt gaaagcaatt ggtgtaacag
     4021 gtttaagaat ggattatcat atttccaatc agcagattgc cgactggtca catcaattga
     4081 aaatcagttt gaatgctagc acgatcacgc ctaaagatat cgatgaattg aaagaagcag
     4141 aagctgattt ctcacagatt gaagcttggc ataattatta tccacgtccc gaaacagggc
     4201 tggacaaaga atggtatcag aaaaaaaatc aatggctcaa aattcaaggt ctccttgtcc
     4261 aaggttttgt accaggagat acagagttga gaggcccact atatcaagga ttgccaactt
     4321 tggaagaaca tcgagggatc catcctcttg caggcgctct agatctatta gcaagtaaca
     4381 cggatattgt ttacatcgga gatgcaggtc tgtcagaaaa cgttcaggag caatttgctt
     4441 ctttccaaaa agaacagaca gtcttgctcc atacggaacc tgtagatgaa gaattttacg
     4501 aatatatatt agggaagcat acgaatcggc aagacgatgc aagagatgtc atacgaagcg
     4561 tagatgcacg ttttcgagag attccgccta tcccagcaag aaatacagct acgcgtatga
     4621 aagggtctat tactttagat aacgaaaagt atttgcgcta tatgggtgag atccaattaa
     4681 ccaaatatga tttgccagcg gatgaaaaag tgaatgtagt agctaaagtt atcaaagaag
     4741 agctaccatt gatcaaccaa atcaaagctg gtatgaacta tcaatttata agaaaagaag
     4801 gaagataaaa atgagcaaaa tcaatttaga gaacctaaca acggaacgca gaaaccaaga
     4861 aacttttgga ttagatgaga tgagtatttc tcaagccgtt caactaatga acaaagaaga
     4921 tcagaaagtg gccgctgcgg ttgaaaaaga actgacaagt atcgaaccag tgattgctaa
     4981 cacgattgag tcctttaaaa aaggtggacg tctgatttat atgggagccg gaacaagtgg
     5041 aagactaggt gtgttagatg ctgctgaatg cgtaccgaca ttcggtgtag aaccggaaat
     5101 ggtcataggt ctaattgctg gtggagaaga agcaatgact gtagctgtcg aaggggccga
     5161 agattcttat gagctagggc aagaagatct aatcaagctg aatttgacaa aaaatgatat
     5221 agttattggt atcgcagcta gcggtcgtac accatatgtg atcggtggat tagattacgc
     5281 aaaatcgatc ggtgctgtca caggaacgat ttcttgcaat aaacaagccg aaatcagtaa
     5341 acacgcggat ttcccaatcg aagtcgatgc aggtccagaa tttttgacag gctctactcg
     5401 attgaagtca ggaacagcac aaaaacttat cttgaatatg atttctacta tcagtatgat
     5461 tggtataggt aaagtattca ataacttgat ggtcgatgtc aaaccaacaa atgaaaaact
     5521 aatcgaacgc tcaaaacgaa tcatcatgca agcgacagaa gctgattacg aaacagctga
     5581 aaagtacttt gaagaagcag atcaaaatgt taagctggct atcgtcatga ttttgacaaa
     5641 tagtgataaa gaggaagctc aagcaaaatt agctgaagca aacggatttg taaaagggac
     5701 cttatgaggg ggaaaagacc atggcaaaaa acgaactgac tttggaagaa ctggcaaaac
     5761 aaatttataa aggggcaggc ggcatgggca atgtagaaag cattgtccac tgcatgactc
     5821 gtgtgcgtat gaagatcatc aatgatgatc ttgtaaatgt ctctgaatta aaagcgattc
     5881 caggagtgct tggtgtagta gaagatgaac agcttcaagt catcatcgga cctgggaaag
     5941 tgaacaaagt agcacagcaa atggttgatt tggcaggcgt gaaattagga gaaaaacttt
     6001 cttctgtaag tacagctaca gcaaattcat cagcatctgg aaaagaccgt gtaaacgaac
     6061 gggcacaaga aatgaaagcg gcacaaaaat cgaaacaaaa accatctaaa atcaaatcgg
     6121 tattaaaaga catttctaat atctttgtgc caatgattcc tgcatttgtc ggtactggga
     6181 tcgtggcagg gattgccgca gttttagcaa acttagtcac agccggaaca cttgatgcag
     6241 caacatggca acaatatatc gatgtgatga acatcttgaa aaacgggatg tttagctacc
     6301 tagttatcta tacaggtatc aatgctgcac aagtttttgg tgcaacacca actcttggtg
     6361 gagttatcgg ggccgttgtg atgctgacag ggatgaatcc ggaagcacca ctgaaaaact
     6421 tatttactgg tgaagctctt gcagctggac aaggtgggat cctaggtgtc atcttcgctg
     6481 tttggctatt gtcaatcgtt gagaaaaaac tgcataaagt agtacctgat gcggtagata
     6541 tcatcgttac accaactctt tcattgattg ccatcggtct gattgaaatt ttcttcatca
     6601 tgcctttagc aggtttcatt tctgatggca tggtcggtgg gatcaactgg gtacttggcg
     6661 ttggtggagc atttgccggc ttcgttctag gcacattatt cttgccgatg gttatgttcg
     6721 gactgcatca aattttgact ccaatccatg tgcaaatgat tgatgaaact ggtcgtacgc
     6781 tattgcttcc aattcttgcg atggctggtg caggacaagt gggtgcagca ttggcattat
     6841 ggattcgctg caaaaaagat aaagaattaa cagaaatgat caaaggagct ttaccagtcg
     6901 ggatcctagg aatcggcgaa ccattgattt acggagtaac attacctcta ggacgcccat
     6961 ttattactgc ttgtatcggt ggaggtatcg gcggggcagt gatcggaatg atcggaaatg
     7021 tcggggctat cgctattgga ccttcaggag ccgctttgat cccattgatc tctgacggta
     7081 aatggttagg ctacatcctt ggattattgg cagcttatgc tggtggcttc gttgctacat
     7141 tcttcttcgg tattcctaaa gaccgtttag aaaaagaaga attagcgact gaaacagcca
     7201 cagtaaatga agtgcctgaa aaagtagtac ctcaaacttc agctgaaagt gtgactatcc
     7261 tctcatcagt tgctgacgga acagttgttt ctttagataa agcaagtgat ccagtttttg
     7321 cacaaaagat gatgggtgaa ggctactttg tagaaccaga aaacggacat atctattctc
     7381 cagttactgg ggtagtcagc tctgtcttcc caacgaaaca cgctattggg atcacaacgc
     7441 caaatggttt agaaatcttg cttcatatgg gaatcaacac agttgactta ggcggtacgc
     7501 catttgatgt gaaagtaaca gaagggcagc aagtgacacc tgatacgctt gtagcagaag
     7561 ctgacttaga agcgatcaaa gcagcaggca aagaaacatc catgatcgtc ttggtaacca
     7621 atatggatcg tgtaaaaaac tttgtcttgg aaaaaacagg taaagcaaaa gcaaaagcac
     7681 ctgtgatgga tgtcaaagct taatatgata gaaacaatag aaacgaaaga agaaactgaa
     7741 atctattttt cagcttcttc ttttttatta tttattgttt tctgctaaaa tctgataaat
     7801 tcgtgtcaca tcagatggtg ttccccgtaa ttcataatct gccaagaaag gaaagccaaa
     7861 tgtttgtgca tattgtttgg ctgttagaca atactgatta ttgaagtttt tatttccgct
     7921 accgactaca ccaagacaaa gtttatgatt gtcttcatgt tccagatatt cacgcatcgt
     7981 ttcggtcaag atttctgtat caccgttatc taaaccattt ccgccgtcta aataagtagg
     8041 aacgaaagta aaaaatggtt ctgtttcttt cgtaaaatca ctattttcat ggatttcttt
     8101 taaagtaact tcaggattga caggatcttc tgcatgcatt ttttcagcat attctgctag
     8161 atgtttggca aaggcacgcg tatttcctga aatcgatata tataaaatgt tcatggatca
     8221 tcctccaatt ctttcctttt agtatagcat cttttttaag ttttttggtg gtgtaatttg
     8281 gaaagggact aggcgctttg cttgtataat gagaaaaaag ccggtagaat tatatttcta
     8341 ttaaagtttt tattaattaa gtaaaaataa aaaaggtttg ttatactttt tgataaaagc
     8401 ggttttattt tggaggatgc ttacatggat acagagcaac agaaaaaagg aattggttta
     8461 attccacttg ctgccttagt aattggttca gcgattggag gtggcgtatt tggtattatg
     8521 acggacatct ccggatcggc tggcggccca gctattttca gttgggtact cgtaggaaca
     8581 gcaatgttga tgctctcctt gtcgatcaat aatatcaata aaaaacgtcc agatttaaaa
     8641 ggcgggatct ttagttacgc agaagaaggt tttggccgtt tcgctggttt tatcagcggt
     8701 tggggttact ggctcacatg ctggttaggc aacgtagctt ttgcaacatt gttgatgagc
     8761 gcattaggat atttcttccc agtatttgaa ggtgggcaaa atctggcatc gatcgtgatg
     8821 agtacagtct ttttatgggg atatgcatgg ctagtgaatc ggggagtcga aaatgctagt
     8881 ttcgtcaatg ctatcgtcac attctgtaaa ttagtgccat tatttttatt tattgtagta
     8941 gctatcagca cgttttcgat ccaacaattc attgataact tttctagcga cattatattg
     9001 aatgcgaatg gacagccgat cccgttttcc agtcaagtat tgagttgcat catggtgatg
     9061 ctttgggtat ttgttggtat tgaaggagcc tctgtcgtag gttcacgagc aaaaaaacaa
     9121 tcagatgttg ggaaagcaac gattctaggg atattcggct tgattattat ttacgtcttg
     9181 gtttccatgc ttccatatgg gacaatgacg atgaatcagc tgcaaacgat caaggcgcag
     9241 cctgctatgg gctatgtgtt tgaaatgatg gttggtaaat ggggagcagt cgtgatcaac
     9301 ggcggtttga tcatttcttt agttggtgtt tggctctcat ggacgatctt accgattgaa
     9361 acaatgggaa acatggctga agacggactt ttgcctaaag catggggaaa actcaacaaa
     9421 aatggggcac caacttatgc tatcgtattg acgacgttat gtacgaatgt ctttttactt
     9481 tcattattat tcactgacag tgcttataac tttgcttaca cacttggtac agcagctatt
     9541 ttcttcacgt ggcttttctt aggattgtac caaatgaaac tttcttatca gcggaaagaa
     9601 tgggtccaat ttttagtagg gcttttagca agtgcctttc aagtatgggc tatgctagtc
     9661 gtagccttta atgaagtaat gcttgttctt gttcttttct tgccaggcat ttatttttac
     9721 ctcaaagcac gtaaggagca aaacgagcct gtacaacata tccacttaga taagatttta
     9781 cttataggtg tgacgatggt cggagtgatt gcaatggttt tatttgccac tgggatgctt
     9841 cacgtataga agtataaaag aagtataaag agaggttgtg agcctgtcgt ttattcaaaa
     9901 acatactagg aaaggagctg tgacacaaat gttgtcacag ctcctttttc agaataaacg
     9961 gtgtttaaga gacagccaat cggcaataag gctgaacgtc tctttcacag cctctttttt
    10021 gatgagtcat catacatttt ttcgcttaaa tacgagatat cctaatgcta agaacaaaac
    10081 gatgtaagct aaattaccga atagcatttg attagtagaa agatgagtca attgcttcat
    10141 gatctcttcg tttccaattt ggttttgcag attcaacata ctgatcggat tccatttgat
    10201 ccactcccat ttttggatga gtgcaataag gataccagat atgattgacg aagcaaaata
    10261 aaagacgatt cctgctgaga tggatgcgcc ggaactattg ataaagcaag caatcatcaa
    10321 cactaaactt aaaatcagcc ataaaccgat ataactaccc aaagtataag tgaacaagct
    10381 ttggatcagt gtttgaccag tagaaacctt ctccgtgaag gaaatcgaag ggaaaagaat
    10441 cagtttcatc aacacagtga caataatagc taaaaggtat aaataaacag aataaataac
    10501 taatgtgatc catttgctta ctaatatttc tcctcgagaa tactttcgat aaaggagatt
    10561 tttcaatgta ccatattgtg cttccatcga aatgatcgtg cttgcagctg caatcatgat
    10621 aaagacaatc caagatgtgg cactaaaaag ctgagcagtc atttcaactg gatcaatgat
    10681 cgtcgtgtat tttttggcta gaagtgctgt tcctatgagc aaaacaacta atagaacaga
    10741 taaaacagca gttgactttt tcttgatcaa tttaaataat tcttgtttta caagcaatgt
    10801 catttttctt cttccccctt tactgaatcc agaatttcca atagagaaga ttccaaatca
    10861 ttcgtcagat gctggacgtc tgtcacatgg atattttgtg cagacaatag ctggatcacg
    10921 gttcctaata attcttcgtt ttcttttaaa agcttgattt tatcaggatc ttccaaagta
    10981 tatccagctt ctgtcaagat cgttcgagca gctagatcat ttgttgtaga gataacgatg
    11041 aaatcttgcg ctgtttggtt caattcagcc attgtacatt gtttgatgat ctttccttga
    11101 ttgatcagga tcagctcatc gaccattttt tcaagctcgc tgagtaagtg actggagatc
    11161 aagaaagagg tgccctgagt agccaagtcg gtaatgattt ttcgcacatc tcttgttgct
    11221 tgtggatcca aaccgttcat cggctcatct aaaataacga tagacggatg gttcagtaag
    11281 gcaagagcaa ttcccaattt ttgtttcatt cccaacgagt aggatttcgc ttttttgtga
    11341 atgtactttt ccatcttgag ctgattcacg atagtgtcga tagcagatac atcgttggtt
    11401 tcagagaata acttcagatg atcatagcct gttaagaatg gatagatacc cgggtattcg
    11461 atcaatgctc ctacttgttc caacccttta tgactagagg gagaaatctc attttcacca
    11521 atacagattg ttccatctga gtaatggatc agtcctaaaa tagctttcat gatcgttgtt
    11581 tttccagcgc cattaggacc tactaatcca acgattttgc caggggataa gctaaaactg
    11641 acatcgctga gggcttgttt gtgaccaaag ttcttggata attcttggac cgaaagactt
    11701 ttcaaaaata ttcactcctt ttctatagat agtacaagca tacacctgat gagtggagaa
    11761 gtcgctaggt cattggactt atttcttatt ttttcataaa aaaattgaat attaaataaa
    11821 aaggcttatt ttttctgatc agtacttatt ccaagtgtct agtggccgct agtaatcttg
    11881 atgccaatga tactgatgag tagcatcccc acaaagaacc aagtggaaag attcagctta
    11941 tcatgaaaca agaccacacc aattatgacc gaaccaactg cgccaatccc tgtccagata
    12001 ggataagaga gactcatcgg caaacttttg attgcctttg acaaaaagta aaaactagca
    12061 atcatgccaa tcactgtgta aaaagaataa ttgattttag aaaaaccatc actccatttc
    12121 atcatcgttg accagaatac ttccaaaatt cctgctatga ttaattccat ccatgccata
    12181 cttacattcc tccttaacta taaaaaaata cacaaaaaga ggaaaatcat cccttcaaag
    12241 acctaatgga atgatcatcc tctttatcct acccggtggc agattgtata tctttttctg
    12301 tagtatagca cacctatcca gatatcggca atctcatact ttttagtaaa gtgtaccaag
    12361 cactgacgga tatatcgtta gcggaaaaga agatgaatgc tttgcaattt catagaaaga
    12421 agttgttatt tattaatcat ttaaaaaaaa cagtaactag accttatttc tatatattat
    12481 cgggcaataa attaaaaaaa gctttacctt acaataagta agataatttt tgtgataaaa
    12541 aaataacgaa aaatatttaa aaatagtgtc tatttcgtga gaaaacaact atatttttta
    12601 tgtgttttgc tttataatta aaaaaatatt gtcattttat ggaggaaaat atatggaaaa
    12661 ttatcttagc cttttaggcg tcttgattgt tattctcggg tttgcattta aattagactc
    12721 gatattgatc gtaatggttg ctttagttgt tacagctttg actggcggtc ttggcattca
    12781 aggaatgtta gaacttttag gtacaagttt tgtaaacaat cgcgggatgg cgatttttat
    12841 catcattatg ttggcaacag gaacgcttga aagaaatggt ctgaaagaat cagcggcttc
    12901 cttgatcaag cgattcaaga aagtatctgc tggtatgatt gtagatattt atgggatctt
    12961 tcgaatgatc tttgcggctt ttaacgtgag ttttggtgga gttgctggat ttgttcgtcc
    13021 aatcatttta ccgatggcat tggggaccat cgaatcaaaa gggttagaag tccatcctaa
    13081 gcatgaagaa gaactaaaag gtatggcttc agctatggaa aatgtctgct ggttctttgg
    13141 ccaagtacta ttcatcggag gagcaggggc gttgctagtc caatcaacac tgaaagatct
    13201 aggatatgac gtaacattgg gacagcttgc tttagtggaa gttccagtgg ctatcacggc
    13261 tttagttgtg gcaagtgtct acttctatct taaagaaaag aaattagcga agaaatatta
    13321 tccaggaaag gggcaataag atggactttt ttacaaatcc tgatatttta ttaggcgata
    13381 aattactgga gatcctttat atcgtgatgg gactcgtatt gatttacact ggagtaagaa
    13441 atttaacaga taaaaccaat cctcatcgtt atgggtcagc ctttttctgg acggtcttag
    13501 gggtagttat tgcaggagga agatggcttc cggcaatcgt gaacggtggc ttgatctttg
    13561 cgatgaccat accagcaatt gctaagaaag tcagcaaagg agaaagccgg cttccatcaa
    13621 aagaatatat ggaaaaaatg tcagacaaac taggaatgaa aatttttatt ccagctttga
    13681 gtatcggtgt ctttgcgatc ttgtttgctc tatttaccaa tttaggtgct ttagtcggcg
    13741 tgggcgtcgg tgtttttgca gccatgctaa tcttgatgtt cttttctcga gataatcgac
    13801 caagtacatt tttagatgat gcagcagata tgctgggcac agtagggccg ttaagcatgt
    13861 tgccaatgct tttagcttct ctaggtgcgg tatttacaag cgctggagtt gggacagtga
    13921 tttccaatgc agtaggtacg atcgttccag aaggaaatgt gaacatcggc atcatcattt
    13981 acgctcttgg catggtgata tttacggtaa tcatggggaa tgcatttgcg gccatcaccg
    14041 tgatgacggt tgggatcggt gcaccattcg tatttagtca tggagcgaat ccagcgttaa
    14101 taggaatggt tgcattgact tgcggttttt gtgggaccct gctgacaccg atggctgcta
    14161 acttcaatat cgtgcctgta gcgatgctgg agatgaaaga caaatacgga gtgatcaaaa
    14221 accagttgtt tattgcgtta tttatgctag ttttccaaat tgcttatatg attctattta
    14281 aataatagaa gagaggggtt gtgacagaat aattgtcgca acctcttcta acattgtagt
    14341 ggaacactat ttttttataa gaatatatta agtttgatct gttatgttat ttaaactatt
    14401 gtttccttta tttacgctgt tcaaggaaaa tggtatgata aaagataacc atatgttaaa
    14461 tcaatcggac ggaggaacaa cagatgaaag tactagtaac aggttttgat ccatttggtg
    14521 gggacaaagt gaatccagcc tatgaagcag taaaaaaaat gccagatgaa atcagcggtg
    14581 ctgaaatcat caaagtggaa gtaccgaccg tattcgaaaa aagcagtcaa gtagtcaaag
    14641 aagccatcca acagcaccaa ccagatgtcg tgatctgtgt cggacaagca ggtgggcggt
    14701 cagcagtctc gttcgaacgt gtcgcaatca atctagcaga agcaagaatc cctgataatg
    14761 aaggaaatca gccattcgat acggctttag aagaaaacgg tccagccgct tactttacct
    14821 cattgccaat caaagcaatg acaaaaaatg tacaagatca tggtctacca gcgtacatct
    14881 cttatacagc aggaacattt gtctgcaatg atatcatgta tcgtttgctt catttgatcg
    14941 aaacacaatt cccggcaatt cgaggtgggt tcatccacgt gccattctca cccgatcaag
    15001 tgatcgatcg tccagtgggt acgccttcta tgtctttaga agacattgct tcttctttga
    15061 cttatgcagt tgaagcagca gtagaaaata aagaagatat ttccggaaat gccggaacaa
    15121 ctcactaatt tagcaagaaa cactggctgg ttgctaacgt tatgttagta gccagctttt
    15181 tgtaattaga aaggaaagaa acagccgtgg aagaatggca atccgtcttt gaagaatggt
    15241 ttccaaaaga aatcagcaaa agctatccca tcaaaatttc taaacaatac acaagcagtc
    15301 aacgttggga gatctatgca aaattaacga aaaaacagcg ggaactagtc gataagcacc
    15361 gccgatatct aatcagctct cgttttatgg aagaacatta tttagctgca acagattggg
    15421 tgttttctga cttcaagatc aatccgtttt ttcgaacaaa acgaagccag caaaaattgt
    15481 attgtgagtg tggtagagaa ttaaaagtgc aatacatcgt taaatcgcca aaaaccggaa
    15541 agatcctaaa actaggcatc aaccactttg ccgatcacct gcatgtctct ccgacagtgg
    15601 cagcttcgat ccatcaggga atgacaaaag ttgatctggc cttagacgaa ttattatggc
    15661 ttaagcagaa gaatatcgat tttcctgaag gactatggca aaaatactgt tttgtcttgt
    15721 accaaaatcg tcggatgaaa cagccatatt taccggatat aaaattagct cagcggctcg
    15781 cagaatttcg ccaagtagaa atgccgattt acatagcgga ttatcaagca ttggaaaatg
    15841 aaatcaagaa aataagtgaa cacatcaatg ggcagtcaaa aaagcgacag ataaaaaaag
    15901 aactatttga cgactttgca gaagaattag taaaggatgt agaagaattc ttgatcaact
    15961 atcgcgcatt tttacgtaaa gattggcaat ccatcgtata tgaagaagtg ccagtacatc
    16021 ctaatgctta ttttgaaaca tttatttccg tgttgagaaa gacaaaacgt caacgaacac
    16081 cagaagtcac tgctcagatg gaatattttg cgaaaaacca acgattcatc caaccaaaaa
    16141 tctatctatt tatttggaag caatattgtc gctatggttt tacagaaggg ttcttcgaca
    16201 gcattcctcg gatagtcaga aatggttttt taaaagtatt gcgtaaagaa cgagaagcta
    16261 tacaatctgc tgataagaaa gaccgtacag tctcaaaaga aaaatggcaa ctagtagtga
    16321 aagatatcca atctggaaat gtgcaagaga caatcgacaa atggaaagga aaacattatc
    16381 gttttactga agctcagaaa caagcgttgg aatattatca gaagttagaa gaatcactgc
    16441 gtttcaatga tgaggcaaga aagtatctga aggaattact ataaaaaa
//
LOCUS       DACEEZ010000064        16271 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20313_2017
            SAMN12639966-rid9879223.denovo.064, whole genome shotgun sequence.
ACCESSION   DACEEZ010000064 DACEEZ010000000
VERSION     DACEEZ010000064.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639966
            Sequence Read Archive: SRR10023158
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 16271)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16271)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741683.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741683.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:44:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,016
            CDSs (total)                      :: 2,962
            Genes (coding)                    :: 2,889
            CDSs (with protein)               :: 2,889
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 36 of 73
            Pseudo Genes (incomplete)         :: 29 of 73
            Pseudo Genes (internal stop)      :: 18 of 73
            Pseudo Genes (multiple problems)  :: 8 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16271
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639966-rid9879223.denovo.064"
                     /isolate="E20313_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(56..712)
                     /locus_tag="IWO89_002045"
     CDS             complement(56..712)
                     /locus_tag="IWO89_002045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HAQ0456772.1"
                     /translation="MDSRVLKEYLDKNDFPIIRKGYHKYLTFEGMEDSHTYILKEGTV
                     KASVISNDGREFNLRYIKDLEIVSLLKDEYSQFIDSPYNIRVESEYAEFYRINRVRFW
                     QNINQDPKLQHYVKEYYRYRLLYSMKKMQQMLLNGKLGAVCTQLYELYDLFGVRTEEG
                     MLIDFSITNEEIAKFCGISTASSVNRILKQLKKDGIITVKDHHIVILNMDLLEDNIIF
                     "
     gene            905..1183
                     /locus_tag="IWO89_002046"
     CDS             905..1183
                     /locus_tag="IWO89_002046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0456773.1"
                     /translation="MNYWMNTIINRLETAYQTRFDMKASLVFLNDAYQNSIELIKAVD
                     ENPTNECEEFLNLFMSTRDLFIRQLVDRYPSNYHDVEVQIQKLKAYSA"
     gene            1202..2410
                     /locus_tag="IWO89_002047"
     CDS             1202..2410
                     /locus_tag="IWO89_002047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAQ0456774.1"
                     /translation="MEKKLFNKYFIGITLINFIVYLIYYLLMVIMAVIAQDTLHASLS
                     QAGFASGIYIIGTLLARLFMGKQLELFGRKFTLRGGAIFYLLSTLAYLYTPTIGALYL
                     VRFLNGFGYGTVSTATNAIVTAYIPASKKGEGINYYGLSTSLAAAVGPFLGMILLNMT
                     DFRFIIWFSIVLVFFVTIACLVFPVKNIPLSEEQKISLRSWNINSFIEKKALFITGIA
                     FLMGLSYSSVLSFLSSYTKVIHLVAAGSFFFVVYALVITFTRPLTGRIFDVKGEQYVM
                     YPSYLFLTAGLFLLSVTTNSVTLLISGALVGLGYGTFMSNGQAVCLKIVREHRISIAL
                     STYFIGLDLGLGVGPYLMGMLKSLTSFRGLYVIAGVIPLICTALYLVNGRKHSHEAEG
                     ELIESLKNEI"
     gene            complement(2558..2923)
                     /locus_tag="IWO89_002048"
     CDS             complement(2558..2923)
                     /locus_tag="IWO89_002048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2512 family protein"
                     /protein_id="HAQ0456775.1"
                     /translation="MKHVKALVVKAIMIWAILWVVLTGLYGVSFMDSTIVGVIIVVMI
                     YVLGDLLILRKVGNIAATIADAGSAAVILWLYLYFMNDTVDIWMKVLIPALLIGVAEW
                     FFHKWLLSQGIVPDERKME"
     gene            complement(3070..3843)
                     /locus_tag="IWO89_002049"
     CDS             complement(3070..3843)
                     /locus_tag="IWO89_002049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HAQ0456776.1"
                     /translation="MLNEVENRQQEIVNYLKNNQFARVTDLIELVNYSEATIKRDLVF
                     LEKSGLIRRVRGGAMLIDNRKIDVPYLMKITKLEEETEKRKIADTASSLIRDDMVLFL
                     DSSTTSLHLVRNLARFDGLQIITNGIVTAAMLSEYTTAKVSVLGGAIVPKRATINGSK
                     AYNDALSYYADLAIMSCRGLDFSQGVTETHEGEAMVKKAFRKQSDRLAVLVTQQKIAH
                     KFMHQAIAFHEIDYLVTSKELTKEQYAKVKEHHIECLYG"
     gene            complement(4017..4877)
                     /locus_tag="IWO89_002050"
     CDS             complement(4017..4877)
                     /locus_tag="IWO89_002050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketose-bisphosphate aldolase"
                     /protein_id="HAQ0456777.1"
                     /translation="MLYTMKELLKVAKENQFAVPAFNICSYDMMKAIIEEAERLEAPV
                     ILEIHPDEIAYLSDEFVAAVRAYAYTSKLPIVIHLDHGGTIEDVLRAIRNGYSSVMID
                     ASLKSFDDNVQITKKVVALAHAVNVSVEAELGTIGNNGSAEGGAETIIYTDPDQAKIF
                     VKETGIDTLAVAIGTAHGLYPKNRTPKLNIDLLKELNQQIDLPFVLHGGSGNPDAEVS
                     EAVKYGVAKVNLSSDLKSVFFNALREILSENPSMYEPNAIYPAANKKVQEVVKHKMEI
                     LNTVGKASLY"
     gene            complement(4877..6295)
                     /locus_tag="IWO89_002051"
     CDS             complement(4877..6295)
                     /locus_tag="IWO89_002051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAQ0456778.1"
                     /translation="MKIVGVTACPTGIAHTYMSAEKLEVTARNLNHQAKFETQGVKTE
                     NQLSEQEIKEADAIILAVDKEIELDRFAGKKVKRVSTSRAIKEPQVVIDEALRDIGVF
                     VVSNEKEPAANEKKPTIYNHFMNGVNYMLPFVIAGGIIIALSFAFGITAADPNSADYN
                     VLAAAFSRIGGDTAFAMMVPALGAGIATSIAGKAGFAPGIVAGLLASTGGSGFLGGMI
                     GGVLAGYICDFLANKISVKKEFSAIYQLIVVPFLSILIIGLLMVFVIEQPITWVLDAL
                     TNWLNSLGNTSGLLFGLLIGIMMAADMGGPINKSTSTFSIGLMSAGVNAPIAACMAAG
                     MTPPLGIALATLLFKHKFTKEERTSGQSCWILGASYITEGAIPFAVSDPIRVIPSLMT
                     GSAVAAGISMAVGVTSMAPHGGIWVMLIPNVMNNLPMYVLAIASGTVTTAFMLGLLKK
                     TVKEKKQDKWTKNENLTNEVEA"
     gene            complement(6352..6801)
                     /locus_tag="IWO89_002052"
     CDS             complement(6352..6801)
                     /locus_tag="IWO89_002052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HAQ0456779.1"
                     /translation="MRFDQLIAEELIVFDPSIDSKGSLFERIGDVLKKTNKVRNVQKV
                     VKALFKREEEISTGIEDGFGIPHAKSKYVTKPALCFFHTAKLTDYSGIDEMPIEYIFA
                     IVVPEKSADMHLEILSTLSRRLMNADFRKGIKEATTPADIIRQISQE"
     gene            complement(7246..7509)
                     /locus_tag="IWO89_002053"
     CDS             complement(7246..7509)
                     /locus_tag="IWO89_002053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type B 50S ribosomal protein L31"
                     /protein_id="HAQ0456780.1"
                     /translation="MKENIHPDYHPVVFMDSTTGFKFLSGSTKTSQETVEWEDGNTYP
                     VIRVEVTSDSHPFYTGRQKFTQADGRVDRFNKKYGLKDANAAE"
     gene            complement(7608..8903)
                     /locus_tag="IWO89_002054"
     CDS             complement(7608..8903)
                     /locus_tag="IWO89_002054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor Rho"
                     /protein_id="HAQ0456781.1"
                     /translation="MSDYLTMAELENKTLKEVYSYAKEFKIPYYSKMNKKELSLAVIR
                     AQAEKQGFYYMEGILDIVGQDGYGFLRPINYGPSAEDIYISASQIRRFSLRNGDKVAG
                     KARPPKESERYYGLMHVESVNGKDPEEAKERPHFPALTPLYPQRQLQLETTPQRLSTR
                     MIDIFSPIGFGQRGLIVAPPKAGKTSVLKEIANGITENYPEVELIVLLIDERPEEVTD
                     LERSVKGDVVSSTFDLQPQNHTRVSELVLERAMRLVEDKRDVVILMDSITRLARAYNL
                     VVPPSGRTLSGGIDPAALYKPKKFFGAARNIEEGGSLTILATALVDTGSRMDDVIYEE
                     FKGTGNLELQLSRELSERRIFPAIDIKKSGTRKEELLMPSEELEEIWKLRKNMTGDSL
                     EYTEQLIRFLRKTKNNQQFFKEFQDVSFGKKNPTRNLKR"
     gene            complement(8900..10171)
                     /locus_tag="IWO89_002055"
     CDS             complement(8900..10171)
                     /locus_tag="IWO89_002055"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HAQ0456782.1"
                     /translation="MKKIVIKGNRPLAGEVTISGAKNSAVALIPAAILADSPVILEGV
                     PDIQDVHSLIEILEIMGATVHFSNNILEIDPREVVSIPMPHGKINSLRASYYFMGTLL
                     GKFGEAVVGLPGGCYLGPRPIDLHVKGFEALGAEVTNEHGAMYLRTENKGLRGNRIFM
                     DVVSVGATINVMLAAVKAKGQTVIENAAREPEIIDVATLLNNMGAKVRGAGTDVIRIE
                     GVETLHGCRHFMIPDRIEAGTYLALAAAVGNGIKVKNVIFEHLESFIAKLQEIGVHMK
                     ISEDEIEVYPSKELKPANIMTYPYPGFATDLQQPLTALLLMTTGTSEIIDTIYAKRVN
                     HVPELARMGADITVEGNMIIVNGPNKLHGTEVVASDLRAGACLVIAGLLAEGTTTIYN
                     VDYILRGYDHIIEKLTALGASIEMIEEVGAE"
     gene            complement(10336..11205)
                     /locus_tag="IWO89_002056"
     CDS             complement(10336..11205)
                     /locus_tag="IWO89_002056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010753191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-bisphosphate aldolase"
                     /protein_id="HAQ0456783.1"
                     /translation="MPVVSGAEFLKAARKGGYAVGGFNTNNLEWTQAILEAAEAKQAP
                     VLIQTSMGAAKYMGGYKICKDLITDLVEAMNITVPVAIHLDHGDYEAALECIELGYTS
                     VMFDGSHLPFEENLKLAKDVVEKAHAKGISVECEVGSIGGEEDGIIGNGELADIEECK
                     QMVATGIDYLACGIGNIHGQYPENWKGLAFDHLQAIADAVGPDMPLVLHGGSGIPQDQ
                     IEKAISMGISKVNVNTEFQLSFAAATRKYIEAGKDKEGKGFDPRKLLAPGKAAIVEDA
                     KSHIDWFGSANKA"
     gene            complement(11497..13107)
                     /locus_tag="IWO89_002057"
     CDS             complement(11497..13107)
                     /locus_tag="IWO89_002057"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CTP synthase"
                     /protein_id="HAQ0456784.1"
                     /translation="MTKYIFVTGGVVSSIGKGIVAASLGRLLKNRGLKVTIQKFDPYI
                     NVDPGTMSPYQHGEVFVTDDGAETDLDLGHYERFIDINLNKYSNVTTGKIYSEVLRKE
                     RKGEYLGATVQVIPHITNEIKEKIMRAAKMTDSDVIITEVGGTVGDIESLPFLEALRQ
                     MKADVGTDNVMYIHTTLIPYLKAAGEMKTKPTQHSVKELRGLGIQPNILVVRTEQPVS
                     QSVKNKLAQFCDVEPEAVIESPDVDTLYSIPLVLQAQGMDQIVCDHLKLDTPEADMTE
                     WKELEERVLNLKKKVRIALVGKYVELPDAYISVVEALKHSGFAYNSDIEIDWIKAQEL
                     TRENVEERLKDADGILVPGGFGDRGVEGKIEAIRYARENDVPFLGICLGMQMACVEFA
                     RNVVGLEDASSAETNPDTANNIIDLMADQENIENLGGTLRLGLYPCKIKKGTKTAEAY
                     DGADVVQERHRHRYEFNNKYRQLFEEQGLVFSGVSPDNRLVEIVELPEKKFFVGCQFH
                     PELISRPNRPQKLIKAFVGASLADHETK"
     gene            complement(14300..15640)
                     /gene="glnA"
                     /locus_tag="IWO89_002058"
     CDS             complement(14300..15640)
                     /gene="glnA"
                     /locus_tag="IWO89_002058"
                     /EC_number="6.3.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I glutamate--ammonia ligase"
                     /protein_id="HAQ0456785.1"
                     /translation="MVKKQITGEEIKRIIEEENVRFLRLMFTDILGTIKNVEVPVSQI
                     DKVLENKMMFDGSSIEGFVRIEESDMYLYPDLSTWMIFPWESAHGKVARLICDIYNPD
                     GTPFAGDPRGNLKRALKDMRDLGFTSFNLGPEPEFFLFKLDEDGGITTTLNDKGGYFD
                     FAPTDLGENCRRDIVLELESLGFEVEASHHEVAPGQHEIDFKYADVVDACDNIQTFKL
                     VVKTIARKHGLHATFMPKPLFGINGSGMHCNMSLFNDEGNVFYDKDGELELSETAYHF
                     LGGLLKHARAYTAVCNPTVNSYKRLVPGYEAPVYVAWSGRNRSPLVRVPESRGLSTRL
                     ELRSVDPSANPYLAMAVLLQAGLDGIRNQITPPAAVDRNIYVMDEEEREAAHIQDLPS
                     TIHNAIKELRKDNVMIDALGQHIFSNFVEAKRLEWAAFRQTVSEWEREQYLELY"
     gene            complement(15668..16060)
                     /locus_tag="IWO89_002059"
     CDS             complement(15668..16060)
                     /locus_tag="IWO89_002059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HAQ0456786.1"
                     /translation="MGEKELRRSMSVFPIGTVMKLTDLSARQIRYYEEQDLVHPERSE
                     GNRRMYSLNDIDMLLEIKDYLSEGLNMAGIKRVYEMAQAKKQEAKNKKPLTDHDVRQI
                     FRDEILAQGGLSKTGHYQSQGLQRQFFD"
BASE COUNT         4639 a         3449 c         2766 g         5417 t
ORIGIN      
        1 cgtttgtttc tctaatactc aggtcaaaac gctgatttca caacctcttt gttcatcaaa
       61 agataatatt gtcttctaac aagtccatat tcaaaataac gatatgatgg tctttaacgg
      121 tgataatacc atcttttttc agttgtttta aaattctgtt tacactgcta gcagttgaga
      181 tgccacaaaa tttggcgatt tcttcattcg tgatcgaaaa atcgatcagc atcccttctt
      241 ctgtccgcac accgaataaa tcatataatt cgtaaagttg cgtacaaaca gctcccagct
      301 ttccgtttaa tagcatttgc tgcatttttt tcatcgaata aagcagacga tagcggtaat
      361 actcttttac atagtgttgt agctttgggt cttggttgat attttgccaa aagcgcacac
      421 gattgattcg ataaaactct gcatattctg attcaactcg aatattatac ggcgaatcga
      481 tgaattgtga atattcgtct tttaaaagag agacgatttc aagatctttg atatagcgaa
      541 ggttaaattc tctcccatca tttgaaataa cacttgcttt taccgttccc tctttcaaga
      601 tatacgtatg ggaatcttcc atcccttcga atgtcaaata tttatgatat cctttgcgta
      661 taatcggaaa atcattttta tccaaatatt cctttaagac tctactatcc aatgatgctc
      721 ccccatccaa actttcatct aaaaagatca tacagtactt taaaactttt taaagtccaa
      781 aggtatttta ctaatgatat taacatttgt taagtacctt tttccatcac ttatgcgatt
      841 tttttccttt tttttattcg ttatactgca gaagtaaaat tttttaagaa tagagggaaa
      901 aaacatgaat tactggatga acacaatcat taatcgatta gaaacagcat atcaaacaag
      961 atttgatatg aaagcttctt tagtcttttt aaatgatgcg tatcaaaact cgatcgaatt
     1021 gatcaaagca gtggatgaga atccaacaaa cgaatgcgaa gaatttttaa atctgtttat
     1081 gtctacaaga gatttattta ttcgccaact tgttgatcgc tatccatcca attatcacga
     1141 tgtagaagtt caaatacaaa aattaaaagc atattcagct taaggggaat gtataacata
     1201 catggaaaag aaattattta ataaatattt tattggaatc acattgatca attttattgt
     1261 ctacctgatt tattatctgt tgatggtcat catggcagtc attgcgcaag acaccttaca
     1321 cgccagcttg agccaagcag gttttgcttc tgggatctat atcatcggaa cattgctggc
     1381 acgcttattc atggggaaac agttagaact atttggtcgg aagtttacct taagaggcgg
     1441 cgcgatcttc tatctccttt caacattagc atatctttat acgccaacta tcggggcatt
     1501 gtatttagtt cgattcttga atggattcgg ctacggtaca gtttctacag ctacaaatgc
     1561 tatcgttact gcatatatcc ctgcatctaa aaaaggggaa ggcatcaatt attatggatt
     1621 aagtactagt ttagctgccg ctgttgggcc atttttagga atgattttgc tgaatatgac
     1681 tgattttcgt ttcattatct ggttttcaat tgtactggtc ttctttgtta cgattgcctg
     1741 ccttgtcttt cctgtaaaaa atattccatt atctgaagaa caaaaaatat ctcttcgctc
     1801 atggaatatc aatagtttta ttgaaaagaa agcacttttc attacaggaa ttgccttttt
     1861 gatgggtctt tcttattcaa gtgttttatc atttttatct tcttatacaa aagtaatcca
     1921 cttagttgct gctgggtcat ttttcttcgt cgtatatgct ttggtcatca catttacacg
     1981 tccattgact ggaaggattt tcgatgtcaa aggagaacaa tacgtgatgt atcctagcta
     2041 tttattctta acagcaggat tatttttact gagtgtaacg acgaatagtg tgacattgct
     2101 tatctctgga gcattagtcg gcctaggtta tggtacgttt atgtcaaatg gacaggctgt
     2161 ttgtttaaaa atcgtccgcg aacatcgaat cagtattgct ttatctacct actttattgg
     2221 tttagaccta ggcttaggcg ttggtcctta tttgatgggg atgctaaaat ctcttacttc
     2281 atttagagga ttgtatgtga ttgccggagt cattccattg atatgtacag cactctatct
     2341 tgttaatggc cgaaaacata gtcatgaagc agagggcgaa ttgattgaat cattgaaaaa
     2401 tgaaatttga tcttgatgaa tagataaaaa aaaccgttca gatctgaaaa taatctgagc
     2461 ggttttttga gtaaaaatag tttatgatta ttcttaatta aacatctatt tatttttaaa
     2521 gctttcattt atcgataaga atagtttaac tgttgattta ttccattttc cgttcatctg
     2581 gtacgatgcc ttgacttagc aaccacttgt ggaagaacca ttctgccaca ccgatcaata
     2641 gagcaggaat caatactttc atccaaatat cgactgtatc attcataaaa tatagataga
     2701 gccacaagat aactgcagct gaaccagcat ctgcgatcgt cgctgcgatg ttccctactt
     2761 tacgtaagat caacagatcc cctagcacat agatcatgac tacaataatt accccaacaa
     2821 tcgttgaatc cataaagcta acaccgtata aacctgtcag cacgacccac agaatcgccc
     2881 agatcatgat tgccttcact accaacgcct tcacatgttt cattttctgc actcctttct
     2941 cctggataaa aaagtaagat tatctaacaa gataaccttt tcattattat accatcggct
     3001 gagagaagcg agcaaagata aggtcctatc taaaaatgag gaagccactt agaacattca
     3061 cgtaataatt tatccgtaca gacattcgat atggtgttcc tttactttcg catactgttc
     3121 ttttgtcagt tctttacttg tcaccagata atcgatttca tgaaaagcaa tggcttgatg
     3181 catgaattta tgtgcaattt tttgctgtgt aactaaaacg gcaagccggt ctgattgttt
     3241 tctaaaagct tttttgacca ttgcctctcc ttcatgagtt tctgttactc cttggctgaa
     3301 atctagcccg cgacaactca ttatcgctaa atccgcataa tatgacaatg catcgttata
     3361 ggcttttgaa ccgttgattg ttgctcgttt cggaacaatt gcccctccca atacagatac
     3421 ttttgccgtt gtatattcac tcaacatagc agctgtgacg atgccattcg taatgatctg
     3481 caaaccatca aatcttgcta ggtttctgac taagtgtaaa gaggttgtac tggaatccaa
     3541 gaataagacc atatcatctc gaatcaaaga agaagctgta tctgcaatct tacgtttttc
     3601 tgtttcttct tctaatttgg tgatcttcat caggtaaggc acgtcaattt ttcgattgtc
     3661 aataagcatg gcgccaccgc gaactctacg gatcaatcca cttttttcca gaaaaacaag
     3721 gtcacgttta atcgtggctt cgctataatt tactaactcg atcaaatcgg tgactcttgc
     3781 aaattggttg ttttttagat aattcacaat ctcctgctgt ctattctcca cttcattcaa
     3841 cataaaatca ccccatttac tttatatagt aacaggaaaa aagaggttgt gacagaagtg
     3901 attggcttcg agaaataaga agaaattttt cgaaaaaact acttctgaac atcgtgtgtg
     3961 gttaagaggg tatgacaaga ctattgtcat accctcttga ccacacattt ttatttttag
     4021 tataaactag ccttgcctac tgtgttcaaa atttccattt tatgtttgac cacttcttgt
     4081 acctttttat tagctgctgg ataaattgca ttcggctcgt acatcgatgg attttcactc
     4141 aatatttctc tcaaagcatt gaaaaagacg cttttaagat ctgagctcaa attcactttg
     4201 gcaactccgt attttactgc ttcgctgact tctgcgtccg gattacctga accgccatgt
     4261 aaaacaaatg gaagatcgat ttgttggttc aattctttca gtaagtcgat atttaatttt
     4321 ggagttctgt ttttcggata caagccatgc gctgttccaa ttgctacagc aagtgtatcg
     4381 attcctgttt ctttcacaaa aatcttcgct tgatcaggat ctgtataaat gattgtttca
     4441 gcacctcctt cagcagaacc attattccca atcgttccaa gctcagcctc cactgagaca
     4501 tttaccgcat gagctaacgc aaccactttt ttcgtaatct gtacattgtc gtcgaatgat
     4561 ttcaaagacg catcgatcat cacggaagaa tagccgtttc gaatcgcccg taagacatct
     4621 tctatcgtac cgccatgatc caagtggata acgatcggca gtttacttgt ataagcatag
     4681 gctcttactg ccgcaacaaa ctcatcactt aaataggcga tttcatcagg atgaatttct
     4741 aagattactg gtgcttctaa tctctctgct tcttcgatga ttgctttcat catatcatag
     4801 ctgcaaatat tgaaggctgg tactgcaaat tgattctctt tagccacttt caataactct
     4861 ttcattgtat ataacattac gcttccacct catttgttag attttcattt ttcgtccact
     4921 tgtcttgttt tttctctttc actgtttttt tcaatagtcc aagcataaag gctgtagtga
     4981 ctgtacctga tgcaattgcc aagacataca taggtaaatt gttcataaca tttggaatta
     5041 gcatcaccca aatccctcca tgaggtgcca ttgaagtaac tcctactgcc atggaaatcc
     5101 ctgcagcgac tgctgaacct gtcatcaaac taggtatcac gcggatcggg tcagataccg
     5161 caaacggtat agcaccttct gtaatatacg aagcgcctaa aatccagcat gattgacctg
     5221 aagtccgttc ttctttggta aatttatgtt tgaataataa agtggctaaa gcaataccta
     5281 ggggaggtgt cattcctgca gccatacaag cagcaatcgg tgcatttact ccagctgaca
     5341 tcaaaccgat cgagaaagtc gaagtcgatt tattgatcgg cccacccata tcagctgcca
     5401 tcatgatccc gataagtaat ccgaacaata gcccggatgt atttcccaag ctattcagcc
     5461 aatttgtcaa agcatccaac acccatgtga tcggttgttc aatcacaaat accatcaaaa
     5521 gcccaataat taagatcgat aaaaagggga cgacaatcag ctgataaata gcagaaaatt
     5581 ctttttttac gctaatttta tttgccagaa aatcacagat ataccctgct agcacgccac
     5641 caatcattcc gccaagaaaa cctgaaccac ctgtagaggc cagtaatccg gcaacaattc
     5701 ctggggcaaa acctgctttt ccagcaatcg atgtagcaat ccccgcgcct aacgcaggaa
     5761 ccatcatggc aaacgctgta tctcctccga tccttgaaaa tgcagctgct aacacattat
     5821 agtctgctga gttgggatca gccgctgtaa tcccaaaggc aaaactcagt gcgatgatga
     5881 ttccgcctgc gatcacaaat ggaagcatgt aattcacacc attcatgaaa tgattataga
     5941 ttgtcggctt tttctcattt gcagctggtt ctttttcatt agacacaacg aatacgccaa
     6001 tatctctcaa tgcttcgtct attactactt gcggttcttt gattgctcgg cttgtagaga
     6061 cacgctttac ttttttcccg gcaaatcggt ccaattcgat ttctttatct actgcaagaa
     6121 tgattgcatc tgcctctttg atttcttgtt cagataactg attttctgtt tttacacctt
     6181 gtgtttcgaa ttttgcttga tggtttagat ttcttgctgt tacttctaat ttttctgccg
     6241 acatataagt atgagcaatt ccagtcgggc aagcagttac accaacgatt ttcatgatta
     6301 ttttcctcct ttttcttcac tatcgatact cggacaaaga ctctactatt ttcattcttg
     6361 actaatctgt ctgatgatat ctgctggcgt agtggcttct tttattccct ttctaaaatc
     6421 tgcattcatc aatcgtctgg agagtgtgct gagaatctct agatgcatat ctgctgattt
     6481 ttcaggaact acgatggcaa aaatatattc aattggcatt tcatctatcc cgctatagtc
     6541 cgttaatttg gctgtatgaa aaaaacacag tgctggtttt gtcacatatt tgctttttgc
     6601 atggggaatc ccaaatccat cttctattcc tgtggagatc tcctcctctc gtttgaatag
     6661 tgccttaacg actttttgaa catttcttac cttatttgtt tttttcaaca catcccctat
     6721 tcgctcaaat aatgatcctt ttgaatctat cgatggatcg aaaacaatca gttcttctgc
     6781 gatcaattga tcaaacctca taaaaaacct ctcccccaca aaaaatgtta acgttttctt
     6841 ggaactgagg tcagtatagc atcgctagct gtaccgttca atattatgaa tgaacagagg
     6901 aaaaagaccc atcatccgcg tgtataagtc attaaaatga acgatctagc tcacaaacaa
     6961 tcgcaagtct ataacaaaac aaaaagcctt ccttgaaagg aaagcttagc ttctaatttt
     7021 aggtaaatgg ttttttactt cgaaataacc cctcttcatt tcgttaatat aaggtgtttt
     7081 tatcaagtgg ataattaaaa ctatatacta gtttttcatt gttttgtgca ttatgatcct
     7141 acgacttttt cagcatcaaa cgaagtttta aattggcaaa aaacataaaa aaacagacta
     7201 tccctatcag atagtctgct aaacgttgag ttatcagcgt ttttattatt ccgcagcgtt
     7261 tgcgtctttg agaccgtatt ttttgttgaa acggtccaca cgtccgtctg cttgtgtgaa
     7321 tttttgacgt ccagtgtaga atggatgtga gtcagaagtt acttcgacac ggatgactgg
     7381 gtatgtatta ccgtcttccc attcaactgt ttcttgtgaa gtttttgttg aacctgacaa
     7441 gaatttgaaa ccagttgttg aatccatgaa tactactggg tggtaatctg gatggatgtt
     7501 ttctttcatg attttttcgc tcctttgccc tgctccattt gctgaaccag agttgatttg
     7561 tcgatttaca acattaacat cttaacatgt ttcagtcaag gttgcaatta tcttttcaga
     7621 tttctggtcg gatttttttt accaaaggag acatcttgaa attctttgaa aaattgctga
     7681 ttgttttttg ttttacgcaa aaagcggatc agttgttccg tatattctaa tgaatctcca
     7741 gtcatatttt ttcgaagttt ccaaatttct tctaattctt cacttggcat caataattct
     7801 tcttttcggg tacctgattt tttgatatcg atggctggga agatccgacg ttcagataat
     7861 tcacgtgaaa gttgtaattc tagatttcca gttcctttga attcttcata gatcacgtca
     7921 tccatacggc ttcctgtatc gactaaagcg gtcgctaaaa tcgtcaaact gccaccttct
     7981 tcgatgtttc ttgctgcccc gaaaaatttc ttaggtttat acaatgcggc tgggtcaatc
     8041 ccgccactca atgtacgtcc gcttggtgga acgactaagt tataggcccg agcaagtcta
     8101 gtaatactat ccatcaagat aacgacatct cgtttatctt cgactaaacg cattgcgcgt
     8161 tccaatacta attcagaaac tcgagtatgg ttttgcggct gcagatcaaa tgtagaagat
     8221 accacgtcgc ctttcacgct tcgttctaga tccgttactt cttccggtcg ttcatcaatc
     8281 agtagaacga ttaattctac ttccggatag ttttcggtga taccatttgc gatttctttt
     8341 aatacagaag ttttccctgc ttttggcggt gcgacgatca atccacgctg accaaaaccg
     8401 attggagaaa agatatcgat cattcgggta gatagtcttt gcggtgttgt ttccagttga
     8461 agctgtcttt gcggataaag aggtgttaag gctggaaaat gcggacgttc ttttgcttct
     8521 tccggatctt ttccattaac gctttccacg tgcatcaaac cataataacg ttccgattct
     8581 ttaggtggac gggcttttcc tgccactttg tccccatttc tcaaactgaa tcgtctgatt
     8641 tgtgaggcag aaatatagat atcttctgca cttggtccat aattgattgg ccgtaaaaaa
     8701 ccgtaaccat cttgaccaac gatatccaat attccttcca tataataaaa tccttgtttc
     8761 tctgcttgtg cacgaatcac agctagagat aattcctttt tattcatctt actgtaataa
     8821 ggaattttaa attctttggc ataactataa acttctttta acgttttgtt ctctagttct
     8881 gccattgtca aataatcgct cattcagctc ccacttcctc tatcatttca attgatgccc
     8941 ctaaggctgt tagtttctcg ataatatgat cataaccgcg taagatataa tctacattgt
     9001 aaatcgtcgt tgtcccttct gccaatagtc cagcaatcac taaacaagca cctgcccgaa
     9061 gatcagaagc cacgacttct gtgccgtgaa gtttatttgg accattcacg atgatcatat
     9121 ttccttccac ggtaatatca gcgcccattc gtgctagttc cggcacatgg ttcacacgtt
     9181 tcgcatagat cgtatcgatg atttctgaag tgcctgtagt catcaatagt aacgcagtaa
     9241 gtggctgctg cagatctgtg gcaaatcctg gataaggata ggtcatgatg tttgcgggtt
     9301 ttaattcttt cgaggggtaa acttcgattt catcttcgct gattttcata tggacaccaa
     9361 tttcctgcaa ttttgctata aagctttcca aatgttcaaa gatcacattt ttcactttga
     9421 taccatttcc tactgcagct gctaatgcta gataggttcc tgcttcgatg cggtcaggaa
     9481 tcatgaaatg ccgacagcca tgtaatgttt cgactccttc gatacggatc acatctgtac
     9541 ccgctcctcg aacttttgcc cccatattat tcagtaaagt tgccacatct atgatttccg
     9601 gttctcgtgc agcgttttca attactgtct gtccttttgc ttttacagca gctaacatca
     9661 cattgatcgt tgctccgact gaaaccacgt ccatgaaaat ccgattgcca cgcaatcctt
     9721 tattttctgt ccgtaaatac atggcaccat gttcatttgt cacttcagca cctagagctt
     9781 caaatccttt gacgtgtagg tcaatcggtc tagggcctaa ataacagcca cctggcaggc
     9841 ctactaccgc ttcgccaaat ttaccaagca gtgttcccat aaaataataa gaagcacgca
     9901 aactattgat ttttccgtgc ggcatcggaa tcgatacgac ttctcgagga tcgatttcta
     9961 gtatgttgtt ggaaaaatgg acagttgcgc ccatgatctc taaaatttca attaatgaat
    10021 gaacatcttg tatgtccggc acaccttcca agatcactgg ggaatcagct aagattgcag
    10081 ctggaatcaa tgcgactgca ctattttttg caccgctgat cgttacttca ccagctaaag
    10141 gtcggtttcc tttgattacg atttttttca tctgtttcca ccttgtttat taatcttttt
    10201 tcatggatat gcttgtattt tatcatagat aaaaaaaaga aacgagcatg gtctgccgtt
    10261 ttaagtaaaa aagaatggag agcgacttca aaagtcgttc tccattccta tatatcgtat
    10321 ctgttatgtg gatgcttacg ctttgttagc tgaaccgaac cagtcgatat gagatttagc
    10381 atcttcaacg attgctgctt tacctggagc taataattta cgagggtcga atcctttacc
    10441 ttctttatct ttaccagctt cgatatattt acgagtagct gcagcaaatg ataattggaa
    10501 ttcagtgtta acatttactt ttgagatacc cattgagata gctttttcga tttgatcttg
    10561 aggaatacct gatccgccat gtaatactaa aggcatgtct ggacctacag catcagcaat
    10621 tgcttgtaag tggtcaaaag ctaatccttt ccagttttct ggatattgac catggatgtt
    10681 accgataccg caagctaagt agtcgatacc tgtagcaacc atttgtttgc attcttcaat
    10741 atcagctaat tcgccgttac cgatgattcc gtcttcttct ccgccgattg aaccaacttc
    10801 acattcaact gagatacctt ttgcatgtgc tttttcaaca acgtctttag ctaatttcaa
    10861 gttttcttca aatggtagat gtgaaccatc gaacattact gaagtatagc ctaattcgat
    10921 acattccaaa gcagcttcgt aatcaccatg gtctaagtgg atagcaactg gaacagtgat
    10981 gttcattgct tcaactaaat cagtgattaa atctttacag attttgtaac cacccatgta
    11041 tttagctgca cccattgatg tttggataag tactggtgct tgttttgctt cagccgcttc
    11101 aaggattgct tgagtccatt ccaagttgtt tgtgttgaat ccgccgacag cgtaaccgcc
    11161 tttacgagca gcttttaaaa attcagctcc tgatactact ggcataataa aattcctcct
    11221 aagttttgta aaactaaatt tgttaagaca attcttaacc aacgacccta ttttaccaaa
    11281 catttcggta gttttctact aaaatcaatg aaaatgaaaa ttttcaagaa gttttttgca
    11341 aaaaataaaa tatccaatag cgctttaatc gaaaaaagtc ttttgctttc tatctttttt
    11401 cctacaaata ataacaaatc agaaaaagag aaaagtgaca agaaaattgc ttatcccctt
    11461 ctcttgctac ttttctcttt ctatcaagtt caaaaattat tttgtttcgt gatctgctaa
    11521 tgatgctccg acaaatgctt tgattaattt ttgcgggcga tttggacgag aaatcaattc
    11581 tgggtggaat tggcatccca caaagaattt tttctctggc agctctacga tttctactaa
    11641 acggttatct ggtgagacac ctgaaaagac tagcccttgt tcttcaaaca attgacgata
    11701 tttgttattg aactcatagc ggtgacggtg acgttcttgc acaacatcgg ctccatcata
    11761 tgcttcagcc gtcttcgttc cttttttgat tttacatggg tataatccaa ggcgtaaagt
    11821 tccacccaaa ttctcgatgt tttcctgatc agccattaaa tcaataatat tgtttgctgt
    11881 atctggattc gtttcagctg atgacgcatc ttctaatcca acgacgttac gtgcaaattc
    11941 gacacaagcc atctgcatac caagacaaat acctaaaaac ggaacgtcat tttcgcgagc
    12001 ataacgaatc gcttcgattt tcccttctac tccgcgatca ccaaatccac caggaaccaa
    12061 gattccatct gcgtctttca aacgttcttc tacattttct ctcgttaatt cttgtgcttt
    12121 gatccaatcg atctcgatgt ctgaattata ggcgaaacca gaatgtttca atgcttcaac
    12181 tactgagata tatgcatctg gtaactcgac atatttaccg acaagcgcaa tccgtacttt
    12241 tttcttcaag ttcaagacac gttcttctaa ttctttccat tctgtcatgt ctgcttctgg
    12301 tgtatctagt tttaaatggt cgcaaacaat ttgatccatt ccttgtgctt gtaaaactaa
    12361 tgggatggag taaagtgtat ctacatctgg cgactcgata actgcttctg gttcgacatc
    12421 acaaaattgt gccaatttgt ttttcacgct ttgagaaaca ggttgctctg tacgtaccac
    12481 caaaatattc ggttggatac ctaaaccacg tagctctttt acactgtgct gcgttggttt
    12541 agttttcatt tctccagcag ctttcaagta aggaatcaat gttgtatgga tatacataac
    12601 attatctgta ccgacatctg ctttcatttg acgaagtgct tcaaggaatg gcaaagattc
    12661 tatatcccct actgttcccc ctacttctgt aatgatcaca tctgaatcag tcattttagc
    12721 tgctcgcatg attttttctt tgatctcatt tgtaatatga ggaataactt gaacagttgc
    12781 tcccaagtat tcgcccttac gttctttacg caaaacttca gaatagattt ttcctgtcgt
    12841 cacgttggag tatttgttca aattgatgtc aataaaacgt tcgtagtggc ccagatccaa
    12901 atctgtttca gcaccatcat ctgttacgaa tacttctccg tgctgatatg ggctcatcgt
    12961 ccccggatcc acgttgatgt atggatcaaa tttttggata gttactttta agccgcggtt
    13021 tttcaataag cgtcctaaag aagctgcaac aatccctttt ccgattgatg aaacgacacc
    13081 gccggtcaca aaaatatatt ttgtcataag ttcaaaaact cctttttaat ttatgtgcag
    13141 gaaaagtctc agacaataaa caaagctccc tattcttcaa taagaatagg gagccgaaat
    13201 ttctgtaaac ttctgaccct ctaaaagggt gcccaaggtg tataatacaa gctgactaag
    13261 ggaaagtcaa gtactcaaat gaatactatc cgcttacaat tttttctttt tcgatatcct
    13321 ttgagaaatt atttcttaag tcctttatga gtgcaaaaaa aatttaaccc acataataag
    13381 cttgatataa acatagctaa cgttaaacct aagcaaatca gaaaatcgtt actgaaaaat
    13441 tttaagtgaa ataatagata aaaaaataaa acaaacatta tgatagataa tatcccacct
    13501 aacaaagaac tactttttcc tacttgcagc aacagtttat atagcttatc gtagaaacaa
    13561 tctttttctt tcacccttat ttacctcctt tacgtaaaaa acgtagtcca cagttctcgg
    13621 atttttggca tatcttttct attaaataat atcaaaaaca aaacaattcc actaattttt
    13681 tcaaagatga cctttttttc aacaaaagtg ccttgtaggg attcattgaa ggtaacgaat
    13741 cacataagta aaaaataagg agatgcgaca aaagtcgtgc ctccttattt ctaaataaac
    13801 ggtgttctcc tgccaactcc tcggtcacaa gtcacaatat ccatcaaaca tgagaagctg
    13861 tttttgaaat tatttcttgt gattcggagt taaatggtag gctcacaacc tctggataaa
    13921 cggtattcaa aagccagttt ctcggtatta agccaaattt atgcaaaaat gtagaaagcc
    13981 attttcacaa aatttttctt aatactcgaa actaaacgtc tttttcaaaa cctcttggta
    14041 aacggtgttc aaaagcttgc atctgcggta ataagttcag ccaattcaaa aacaagaaaa
    14101 gctgtttttg cttggctatt cttattactt gttgcagggc agctttttca caacctctcc
    14161 actaaacggt gttcaatcag ctgaccctcg gtattagctc aaaaaacggc aaaatatgag
    14221 gaactatttt tccgttagtt tctctaatac tcaggtcaaa acgctgattt cacagcctct
    14281 aattcacggt attcctgttt taataaagtt ctagatattg ttctctttcc cactcggaaa
    14341 cagtttgtct gaatgccgcc cattcgagac gttttgcttc aacaaaattt gagaagatat
    14401 gttgtcctaa tgcgtcaatc attacattat ccttacgcaa ttctttgata gcattatgga
    14461 tagttgaagg taagtcctgg atatgtgcag cttctctttc ttcctcatcc attacataaa
    14521 tgttacggtc aacagctgct ggcggtgtga tctgattacg gataccgtct agtcctgctt
    14581 gcaacaatac tgccattgcc aagtatgggt ttgcagatgg atcaactgac cgcaattcca
    14641 aacgtgtgga taaaccgcga gattctggta cacgtactaa tggcgaacga ttacggccac
    14701 tccatgcgac ataaacaggc gcttcataac ctggaacgag acgtttataa gaattgactg
    14761 ttggattgca aacggctgtg taggcacgag catgtttcag caaaccacct aagaaatgat
    14821 aagctgtttc acttagttcc aattcaccgt ctttatcata gaaaacattt ccttcatcat
    14881 tgaacaaaga catattgcaa tgcataccag aaccattgat cccaaataac ggtttcggca
    14941 tgaaagtcgc gtgaagtcca tgcttgcgtg caatcgtttt gacaacgagc ttgaaggttt
    15001 ggatattatc acaagcatct accacatctg catatttaaa gtcaatttca tgctgtcctg
    15061 gcgctacttc atgatgtgaa gcttccactt caaagcctag actttctaat tccaacacga
    15121 tgtcacggcg gcaattttca cctaaatctg ttggtgcaaa atcaaaataa ccacctttat
    15181 cattcaaggt cgttgtgatc ccaccgtctt cgtctaattt aaataagaag aattctggtt
    15241 caggtcctaa attgaaagag gtaaaaccaa ggtcgcgcat atctttcaat gctcgtttca
    15301 aattcccgcg aggatcgccg gcaaatggtg tcccatccgg attatagata tcacaaatta
    15361 aacgagcaac ttttccatga gcactttccc acgggaagat catccaagtg gagagatctg
    15421 gatacaaata catatcgctt tcttcaattc gaacaaatcc ttcaatcgaa gaaccgtcaa
    15481 acatcatttt attttctaat actttgtcga tctggctcac tggaacttct acatttttga
    15541 tcgtacctaa gatgtctgtg aacatcaatc gtaaaaatcg tacgttttct tcctcaatga
    15601 ttcgtttgat ttcttcacct gttatctgtt tctttaccat ggtatccctc ttcctttttt
    15661 aatttattca gtcaaagaat tgtctttgca atccttgcga ctgataatga cctgttttgc
    15721 ttaacccacc ttgcgccaaa atctcatcac gaaaaatttg tcgaacgtca tgatctgtca
    15781 acggcttctt gttttttgct tcttgttttt tcgcctgtgc catttcgtat acacgtttga
    15841 tcccagccat attcaatccc tctgaaagat agtctttaat ctcaagcaac atatcaatat
    15901 cattcagcga atacatacgt cggtttcctt cgcttcgttc cggatgaact aaatcttgtt
    15961 cttcataata gcgaatttgc cgagcagaca aatcagtcag cttcataact gtaccaattg
    16021 gaaatacgga catagatcgg cgcaattcct tttctcccat cagacgtccc ctttcgtttt
    16081 ataagttggc taaagtgtac caattaaaaa aaatattgtc aactgcatat gttaggtttt
    16141 ctaacatgag atctctattt tttataaaat aacaaaaaaa taagaacaca aaaaaggtat
    16201 ctagttgatt ttcgtaccag atacctttta ttttttatta tactgtacta tcctttatta
    16261 gtgtttttat t
//
LOCUS       DACEEZ010000065        16195 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20313_2017
            SAMN12639966-rid9879223.denovo.065, whole genome shotgun sequence.
ACCESSION   DACEEZ010000065 DACEEZ010000000
VERSION     DACEEZ010000065.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639966
            Sequence Read Archive: SRR10023158
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 16195)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16195)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741683.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741683.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:44:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,016
            CDSs (total)                      :: 2,962
            Genes (coding)                    :: 2,889
            CDSs (with protein)               :: 2,889
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 36 of 73
            Pseudo Genes (incomplete)         :: 29 of 73
            Pseudo Genes (internal stop)      :: 18 of 73
            Pseudo Genes (multiple problems)  :: 8 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16195
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639966-rid9879223.denovo.065"
                     /isolate="E20313_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            363..730
                     /locus_tag="IWO89_002060"
                     /pseudo
     CDS             363..730
                     /locus_tag="IWO89_002060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329268.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
     gene            910..1851
                     /locus_tag="IWO89_002061"
     CDS             910..1851
                     /locus_tag="IWO89_002061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-related sulfurtransferase"
                     /protein_id="HAQ0456787.1"
                     /translation="MDYQVLLYYNYTKIEEPEEFAKNHLAFCKSLDLKGRILVAEEGI
                     NGTVSGTVENTTAYMKAMKADPRFADTMFKIDPSEGHAFKKMFVRYRPELVSLKLEED
                     IDPNQVTGAYLSPQEFKEAILDEQTVVIDARNDYEYDLGHFRGAVRPDIRSFRELPDW
                     IREHKEEFMEKRVVTYCTGGIRCEKFSGWLVREGFKDVGQLHGGIATYGKDPEVQGEL
                     WDGKMYVFDERIAVDINQVDKQIVGRDWFDGTPCERYINCSNPECNRQILASIENEEK
                     HLGACSIECAKHPRNRYVQDHGISPEEAELRFTALIK"
     gene            1920..2651
                     /locus_tag="IWO89_002062"
     CDS             1920..2651
                     /locus_tag="IWO89_002062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine protein phosphatase"
                     /protein_id="HAQ0456788.1"
                     /translation="MKSLIYAIGDVHGKHELFEKLIHYYDPKIHQLVLLGDLNDRGPR
                     TKECFLLGKQLVEETGAIYLRGNHEEYFLQFLQSPEDWYAPYMRNGGKETFESLIHPG
                     ASEEYSPTEISMMIQSRYSELIKFLVQRPLYYEWENYLFVHAGVDLTKKDWRKTSLRD
                     FIWIREPFHEGKNNTGKTIVFGHTITPMLHGDMQTTDLWIQDHKIGMDGGAVFGGSLH
                     GVVFDKEGILQDIEYQNNEGPWHGI"
     gene            2795..4975
                     /locus_tag="IWO89_002063"
     CDS             2795..4975
                     /locus_tag="IWO89_002063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding transcriptional accessory protein"
                     /protein_id="HAQ0456789.1"
                     /translation="MTENLNQTIIQLVQKELSDYRPKQLTTVLNLLNEGNTVPFIARY
                     RKEMTGSLDEVQIREIEERYAYLENLEKRKTEVIRLIDEQGKLTPELEAEITQAVKMQ
                     QVEDLYRPYKQKRRTKATIAKEKGLEPLAKWLMQLTDGEVQSEAEKYIDKEKEVSSAE
                     EALHGAHEIIAEQVSDNAKFRTWIRSYTYNKGMYVSNVKDEQADEKGVYEMYYDFAEP
                     VHKMVSHRILATNRGEKEDILKVFFQVDEAAILAYLDRQIVKNPASPSSSFVREAYQD
                     SYKRFIQPAIERELRNELTEKADEQAIAIFGENLRNLLLQPPLKGKVVLGFDPAYRTG
                     CKLAVVDATGKVLAIEVIYPHKPAAQAKREAAGPAFIQLINKYQVDMVAIGNGTASRE
                     SELFVAEQLKSADHKTYYAIVNEAGASVYSASEIARKEFPHLQVEERSAVSIARRLQD
                     PLAELVKIDPKAVGVGQYQHDVSQKRLAEQLDFVVETAVNQVGVDVNTASPQLLQHIS
                     GLNKTTAQNIVIYREENGEFTARTQLKKVPRLGPKAYEQAIGFLRVPGGKNILDNTGI
                     HPESYSIAKEILTSVQLSEKELGTEEAVEKLTRLSVEKLAESLSVGEETLADILAGLT
                     QPGRDMRDEMPAPLLRTDVLSMEDLKPGMELTGTVRNVIDFGAFVDIGVKQDGLVHIS
                     KLSKKFVKHPTDVVSVGDIVTVWIEQVDTKKGRISLTMLSPYEE"
     gene            4995..5477
                     /locus_tag="IWO89_002064"
     CDS             4995..5477
                     /locus_tag="IWO89_002064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SprT family protein"
                     /protein_id="HAQ0456790.1"
                     /translation="MTQEELQQLVEEISLKSFQLPFKHQAMFNPRLRTTGGRYHLNDH
                     HLDFNAKLFEESDEETIAGIIKHELCHYHLHLAGRGYQHKDMDFKYLLKKTGGLRYAP
                     ALTKKQIKMEYYRCQDCSATICRKRRIDVRKYRCGRCHGEIKWIETKREDFNEAQKIR
                     "
     gene            5493..6254
                     /locus_tag="IWO89_002065"
     CDS             5493..6254
                     /locus_tag="IWO89_002065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcF family protein"
                     /protein_id="HAQ0456791.1"
                     /translation="MEKSEQRNLKDWNQLLNFLAAPEEGNFEELTAPTLILAGNCLPI
                     LADKAAQMYLNGQVDQLFLVGGVGHATRILYENFEKQGFHFEDGMSESEICRQYLKEV
                     YDLPDKAFLIESKSTNSGENATFSLEILHSLGAVPEKILLMNDPTLQRRTRATFEKVW
                     QNEQTIFANAVPFVPEILHFSEEIIFTAKELNHQWPKEYFHALVLGEMERLHDDENGY
                     GPKGKDFIPHIDISEEVWSSYTRIKESIKTDFSRT"
     gene            6385..7719
                     /locus_tag="IWO89_002066"
     CDS             6385..7719
                     /locus_tag="IWO89_002066"
                     /EC_number="6.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate--L-alanine ligase"
                     /protein_id="HAQ0456792.1"
                     /translation="MENQNKLYHFVGIKGSGMSSLALVLHEQGLNVQGSDIEKYFFTQ
                     RDLEKANITILPFNADNVKPGMTIIAGNAFPDSHEEIQRAKELGLEVIRYHDFIGHFI
                     QNYTSIAVTGSHGKTSTTGLLSHVLSGVRPTSYLIGDGTGHGDPQAEFFSFEACEYRR
                     HFLAYSPDYAIMTNIDFDHPDYYTSIDDVFTAFQTMAGQVKKAIFAYGDDAYLRKLKA
                     NVPIYYYGVTENDDIQARNIERTTSGSAFDVYHGDEFVGHFTVPAFGKHNILNALGVI
                     AVAYFEKLDLKEVAEEMLTFPGVKRRFSEKIVADMTVVDDYAHHPAEIKATIDGARQK
                     YPDKEIIAVFQPHTFTRTIALMDEFAEALDLADKVYLCDIFGSAREEQGNVKIEDLGA
                     KIKKGGEVIKENNVSPLLDYHDAVVIFMGAGDVQKFEQAYEKLLSSTTKNVL"
     gene            8147..8632
                     /locus_tag="IWO89_002067"
     CDS             8147..8632
                     /locus_tag="IWO89_002067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dehydratase"
                     /protein_id="HAQ0456793.1"
                     /translation="MNIGKPRKLGKTINEIEEGDSLSLTESIEDNQLLLYLGLTNDAN
                     PLYIQHDFAQKTEYQQPIVPSVMLMGIITSAISKHLPGPGSHVVNFSVNFIEPVFHYE
                     TLTFQFEVIKVDKMKDVVTISVEAENTNEDRVLDAVVMVQPPQVPELEIAIESEGELN
                     E"
     gene            8625..9308
                     /locus_tag="IWO89_002068"
     CDS             8625..9308
                     /locus_tag="IWO89_002068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Bax inhibitor-1/YccA family protein"
                     /protein_id="HAQ0456794.1"
                     /translation="MNNPSTSMSGINRFYAKVYGFLGMGLALSAVTSYLALWVFPLQL
                     ATFINNFPLGFVGLWIVELILVMVLGVKAQKNPSLAIGGFIAYSLLNGITLAVTLAYY
                     DIGTVTQAFLTATLMFMGMSVVGVTTKRDLSGVGRAGQAALIGVIIALVLNIFILRSS
                     VVELFISLLLVVIFAGITAYDNQSIRNIYVQTNGTAHSGIAVFMALKLYLDFINLFLA
                     FLRIFGRNN"
     gene            9434..12079
                     /gene="polA"
                     /locus_tag="IWO89_002069"
     CDS             9434..12079
                     /gene="polA"
                     /locus_tag="IWO89_002069"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HAQ0456795.1"
                     /translation="MTKNKLLLVDGNSVAFRAFFALHNSLERFKNRNGLHTNAIYAFN
                     TMFENVMQKEQPTHVLVAFDAGKTTFRTEMYAEYKGGRSKTPGEFKEQMPYIRDLLTG
                     LGVQYYELPNYEADDIIGTLAEKVDKDQFDVVVLSGDRDLTQLASKEVKVDITVKGVS
                     DIESYTPEHVAEKYDGLTPKQIIDMKGLAGDASDNIPGVTKIGEKTAIKLLKQYGSVE
                     GIYEHIDEMKQSKMKENLINDKEQAFLSKKLATINTSAPVDVSIDSLKYEGKNLDKLV
                     PFYKEMDFNQFLSKLNIVEEEVKMDDILFEVVHEFKEEMFTTDMALYVEMMGDNYHTE
                     EIVGVAWGTEKKIYVTNDLSVFENRAFHSWITDPTRLKKVYDAKRTYVALNRYTGKTK
                     GIDFDVLLGAYLLDTNEKSTDIEGIAAHYGYNDIQSDEAVYGKGAKKGLPEEEELFFA
                     HLARKVAAINALTPKLSQELADKNQEDLFRKMELPLSIILAEMEISGIKVDATRLQEM
                     KGEFSARLREIEQKIYEEAGEEFNLNSPKQLGVILFEKMGLPVIKKTKTGYSTAVDVL
                     EQLREQAPIVEDILTYRQIAKIQSTYVEGLLKVIQSDGKVHTRYVQTLTQTGRLSSVD
                     PNLQNIPIRLDEGRKIRQAFVPREKDWLIYSSDYSQIELRVLAHISNDEHLKAAFLEG
                     QDIHASTAMRVFGIEKAEDVTPNMRRQAKAVNFGIVYGISDYGLSQNLGISRKAAQQY
                     IDTYFEKYPGVKEYMERIVREAKDQGYVETLYHRRRYLPDINSRNYNIRSFAERTAIN
                     TPIQGSAADILKIAMIELDKRLKETGLQATMLLQVHDELVFEVPEKELESLDKLVKEV
                     MEQAVSLHVPLITDSSWGKTWYEAK"
     gene            12124..12960
                     /gene="mutM"
                     /locus_tag="IWO89_002070"
     CDS             12124..12960
                     /gene="mutM"
                     /locus_tag="IWO89_002070"
                     /EC_number="3.2.2.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-formamidopyrimidine glycosylase"
                     /protein_id="HAQ0456796.1"
                     /translation="MPELPEVETVRKGLERLVVGKTIQKVQVLWPKIIEQPETPIFEA
                     SLVGETIQSIGRRGKFLIFHLDHCELISHLRMEGKYQFTKENTPIDKHTHVLFFFEDG
                     SQLRYNDVRKFGRMTIVEKGASATYRGIMKLGPEPLPDSFLLADFANGLKKSHKAIKP
                     LLLDQRLVTGLGNIYVDEALWEAKIHPEQPANTLRPKEVEQLRLSIIDVLDRAIEAGG
                     TTIRSYLNALGESGGFQVALHVYQQTGKPCIRCGTPIVKTKVAQRGTHYCPNCQRLKI
                     AK"
     gene            12924..13553
                     /locus_tag="IWO89_002071"
     CDS             12924..13553
                     /locus_tag="IWO89_002071"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HAQ0456797.1"
                     /translation="MSELSKAKNRKVGFVLGLTGGIATGKSTAAKVFQSHGFPLIDGD
                     VIARETVEPGTLALRKIVSVFGQEVLQPDGQLDRGKLGMIVFPSKEKRQKLDQLLDPF
                     IRKAIKDQIASLSSNHPLVIVDIPLLYEGHYDHYMDAVAVVYTTPETQLQRLMKRNQL
                     TLEQAQQRISSQLPIEEKKQRADILFDNNGTKEKLVGQIENWLFANRFL"
     gene            13605..13937
                     /locus_tag="IWO89_002072"
     CDS             13605..13937
                     /locus_tag="IWO89_002072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HAQ0456798.1"
                     /translation="MGSLKNQKDKVVGNAKEKIGEMLNKENLIDEGRTQSETAEEKEK
                     QYQARIDEIEDQQAEEEAKRQDLTQERKNPTPDRSKTPLPESDHISDEQKIEEQRMKH
                     YTGIEEKL"
     gene            14249..14740
                     /gene="nrdR"
                     /locus_tag="IWO89_002073"
     CDS             14249..14740
                     /gene="nrdR"
                     /locus_tag="IWO89_002073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor NrdR"
                     /protein_id="HAQ0456799.1"
                     /translation="MRCPRCQHNNSRVIDSRQADDGRAIRRRRECENCSFRFTTFERI
                     EAAPLLVIKKNGEREEFNRDKILRGLIRSAEKRPVAMEQMEQIVNHVENRVRSLGENE
                     VSTNLIGEYVMEDLVDLDEIAYIRFASVYRQFKDMSVFLKELQDIVDKAKENNKENNK
                     ENN"
     gene            14763..16136
                     /locus_tag="IWO89_002074"
     CDS             14763..16136
                     /locus_tag="IWO89_002074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Replication initiation and membrane attachment"
                     /protein_id="HAQ0456800.1"
                     /translation="MENAWKELQPKNIYQGRKAAPLREDSKNALLYLYQPLIGGEALS
                     IYFTLLSEISQESGKGPEGMHADLLSSLGCGIPQFYDARKKLEGIGLLDVYFKEDSDL
                     GPRFIYELLEPLIPKAFFKDSVLAFLLLEKVGERRFQKLTQFFEPKRHTAEGYQKITK
                     KFLDVYRFNEAVYAANQENIESLNKSFEESESVALLEADSTFDWQFLNDWLAKKHIAS
                     LDDPIIKQVDMYHQLYGFDELTLGELISQSYDFSEQKVSEKELQKAILAMQRRPVKQT
                     KETVAVSENSNEPSSNEHLTNAALQLIKEAQSIPPMKYLEAIKSEKGGYVSKQENWLL
                     QDLVSQSGLSSSVINVLLNYVLVIKNNASLNASYVNTVANEWAQKKIATAEEAIQHIR
                     QISNQAKQSKQKKQTNYQTGKRNVRREKLPDWVNQPKDEKQISSEKKAEIDRRFKEYL
                     AKKEGDI"
BASE COUNT         5501 a         2838 c         3545 g         4311 t
ORIGIN      
        1 agaaagaggt cgtgagcctg ccgtttcact ctgagtcaca aagagcaatt tcaaaaaata
       61 gcttctcatg tttgttgaat attgtgtctt gtgaccgagg agttgtcagg agaacaccgt
      121 ttaccagagg ttgtgacttg tttttagtca cgacctcttt ttttatttat tccatataaa
      181 tacacattat ttattccgaa aagtgaaagc gtaaataaca aaaacaacca acttatttat
      241 tattaaagaa aagcgtattt aagtaggacg attctgttga gagctttcgt agattttccc
      301 attttcctat ggtaaactga caaagttgag aatgttagaa cagatcgttt aggaggaaac
      361 agatgtttac gaatgaaata aaaatcatgc tttatgtgga cgatgtagaa aaaaatgcaa
      421 cgttttggag agcagttggc tttcggaaaa agatcgtcaa gaaatggatg ggacgcttat
      481 aattgaagta ggagttgagg acagtcaaac ctcatttctg ttatatgatc gagcatttgt
      541 tgagtcccat tcacctggca cagctactag tgccccatct ttgatgtttt acagtaaaga
      601 tatctttgga ctatataaaa aaatgcagac gcaatcagtc acattaggtg atatggtaca
      661 gcttggagaa gaatatgtct ttaactttgc tgatcccgat ggcaactact ttgcggttac
      721 aggtaaataa tagaaataaa gactttgttt ttctgtgcga aacaggaaag cgaagtcttt
      781 ttaattcaaa taataatatt atgtaaacaa aaaactttcc atgcgtaatt atacacaagc
      841 aaataaatcg tcaaatgcga acgccttctt tatactgttt aggaacgaaa agaaggaagg
      901 aagaaataca tggattatca agtactgctt tattacaatt atacaaaaat cgaagaacca
      961 gaggaatttg ccaaaaatca tttagccttt tgcaaatctt tggatcttaa aggtcgtatt
     1021 ctagtagctg aagaaggaat aaatggtact gtgtctggaa ccgttgaaaa cacgactgcc
     1081 tatatgaaag caatgaaagc tgatcctcga tttgctgata cgatgttcaa gatcgatcca
     1141 agcgaaggac atgcctttaa gaagatgttt gtaagatacc gtccagagct tgtaagttta
     1201 aagctagaag aggacattga tccaaaccaa gtgacaggtg cctatctttc accgcaagaa
     1261 tttaaagaag ctattttaga tgaacagaca gtggtgattg atgcacggaa tgattatgaa
     1321 tatgacttgg gacattttcg aggggctgta cgcccagata tacgcagttt tcgagagctt
     1381 ccagactgga tccgcgagca caaagaagaa tttatggaaa aacgggtagt gacctactgt
     1441 actggtggca ttcgttgcga gaagttttct ggttggcttg tccgtgaagg attcaaagat
     1501 gtcggacagc tgcatggcgg tattgctact tatggtaagg atcctgaagt ccaaggagaa
     1561 ttatgggacg gcaagatgta tgtgtttgat gaacggatcg ctgtcgatat caaccaagta
     1621 gataaacaaa tcgtcggtcg cgactggttt gacggaacac catgtgaacg atatatcaat
     1681 tgtagcaatc ctgagtgcaa tcgtcagatt cttgcttcca ttgaaaatga agaaaaacat
     1741 ttaggagcat gtagcataga gtgtgcgaaa catccgcgca atcgctatgt tcaagaccat
     1801 ggaatctctc cagaagaagc agaactgcgg tttacagctt taattaaata gaaaacaata
     1861 gttttgattg tcgaccattt ccaatatgcg ctatactagc gttgaggatg gtgatccaca
     1921 tgaaatcatt gatttatgct attggggatg tccatggcaa acatgagctt tttgaaaaac
     1981 ttattcacta ttatgatcca aagatccatc agcttgtttt attaggggat ctcaatgatc
     2041 gaggtcctcg tacaaaagaa tgttttttat taggaaagca attagtcgaa gaaacaggag
     2101 ccatttacct ccggggaaac catgaagaat attttttgca atttctgcaa tcaccggagg
     2161 actggtatgc gccttatatg agaaatggtg gaaaagaaac gtttgaaagc ctgattcacc
     2221 caggggcatc ggaggaatac tccccaactg aaatctcgat gatgatccaa tcgagatatt
     2281 cagaactgat taaattttta gtccagcgcc ccttgtatta tgaatgggaa aattatcttt
     2341 ttgtccatgc cggtgtggat ttgacaaaga aagactggcg taagacaagt ctaagagact
     2401 ttatttggat aagggaacca tttcatgaag ggaaaaacaa tacaggtaag acaattgtat
     2461 ttggacatac gattacacca atgctccatg gagatatgca gacaacagac ctctggattc
     2521 aggatcataa aatcggaatg gacggtgggg ctgtattcgg agggtcattg catggggttg
     2581 tttttgacaa ggaaggtatt cttcaagata ttgaatatca gaacaacgaa ggaccttggc
     2641 atggaatctg actaatggaa agaatgaagg agctgtgact ttgttgcggc tctttttatt
     2701 tttatagaga atattgtttc tttcagtagt ttaacccagg ataaaaagta tgatagaata
     2761 aagaagcaat ttttagcaga aaagaggcat tttaatgacg gaaaatttga accagacgat
     2821 cattcagctt gtacaaaaag aactttcaga ctatcgtcct aagcagttga ctactgtact
     2881 taatttgtta aatgaaggaa acactgttcc ttttatcgcg cgttaccgaa aagaaatgac
     2941 tggaagtctt gatgaagtcc agattcgtga aatcgaagag cggtatgcct atcttgaaaa
     3001 cttagaaaaa cgtaaaactg aagtgattcg gctaatcgat gaacaaggga aactgactcc
     3061 cgagttagaa gcagaaatca cccaagccgt gaaaatgcaa caagtggaag atttgtatcg
     3121 tccatacaaa caaaaacgac ggacaaaagc aacgattgct aaagaaaaag gacttgaacc
     3181 actcgcgaag tggctgatgc agcttacaga tggagaggtg caatctgaag cagaaaaata
     3241 tattgacaaa gaaaaagaag tctcttctgc cgaagaagcg ttgcacggtg ctcatgagat
     3301 tattgctgaa caagtcagcg ataatgcaaa atttcggact tggattcggt cttatacgta
     3361 taacaaaggt atgtatgtca gcaatgtcaa ggatgagcaa gcagatgaaa aaggcgtata
     3421 tgaaatgtat tatgactttg cagaacccgt ccataaaatg gtttctcatc gaatattagc
     3481 tactaatcgt ggagaaaaag aagatattct gaaagtattc tttcaagtag atgaagcagc
     3541 aattttagct tacttggatc gccaaatagt caaaaatcct gcctctcctt cttcttcatt
     3601 cgtcagagaa gcctatcagg atagttataa acgctttatt cagccagcaa tcgagcgaga
     3661 actgcgcaat gaattgactg aaaaggcaga tgagcaagca atcgctattt tcggcgaaaa
     3721 cctccgtaat cttttgttgc aaccgccact aaaaggtaaa gttgtcctgg gatttgatcc
     3781 ggcttatcgt actggatgta aactagcagt cgtagatgcg acagggaaag ttttagcaat
     3841 cgaagttatt tatcctcata aacctgcagc gcaagcaaaa agagaagctg ctggaccagc
     3901 ttttatccaa ttaattaata aatatcaagt agatatggta gctatcggaa atggtacagc
     3961 cagtcgggaa tctgaacttt ttgtggctga gcagttgaaa tccgccgacc ataaaaccta
     4021 ttacgctatc gtcaacgaag caggcgcttc tgtttactcc gctagcgaaa ttgcacgaaa
     4081 agaatttccg catctacaag ttgaagaaag aagtgctgtt tctattgccc gtcgcctgca
     4141 agatccttta gctgaattag tcaaaatcga tcctaaagct gtcggagtag gccagtacca
     4201 acatgacgtt tctcaaaaac gtttggctga acaattggat tttgttgtgg aaacagcggt
     4261 taaccaagta ggtgtggatg tgaatacagc aagcccgcag ctcttgcagc atatatcagg
     4321 attgaataaa acaactgccc aaaacattgt catttatcgt gaagaaaacg gagaattcac
     4381 cgcacgaact caattgaaaa aagtgccacg cttaggacca aaagcatatg aacaagcaat
     4441 cggattcttg cgagtccctg gtgggaaaaa cattttggac aacacaggta tccatcccga
     4501 aagttattct attgcaaaag aaatattaac ttcagtccaa ctttcagaaa aagagcttgg
     4561 tacagaagaa gcagtagaaa aattgacacg attatccgta gagaaactgg ctgaaagcct
     4621 atcagtcgga gaagaaacgt tagccgatat tttagctggg ctgacacaac ctggacgaga
     4681 tatgcgtgat gagatgccag cgcccttatt acgtacagat gtattgagta tggaagactt
     4741 gaaaccaggg atggaattga ctggaacggt ccgtaatgtc attgacttcg gcgcatttgt
     4801 tgatatcggt gtgaagcaag acggacttgt ccatatatcg aaactcagca aaaaattcgt
     4861 caagcatcct acagatgtcg tgtcagtagg agatatcgta acagtttgga tagaacaagt
     4921 cgatacgaaa aaaggcagaa tcagtttgac gatgctttcc ccttatgaag agtaacaatg
     4981 aggagtaaca aaaattgaca caagaagaac tgcagcagtt agtagaagag atatcattaa
     5041 aaagcttcca gcttcctttc aagcaccaag caatgttcaa tccacgctta cgtacaactg
     5101 gcgggcgcta tcatttaaac gatcatcatt tagattttaa tgcgaagcta tttgaagagt
     5161 cagacgaaga gacgattgca ggaatcatca aacatgaact ttgccattat catcttcatc
     5221 tagctggaag agggtatcag cataaagata tggatttcaa gtatcttttg aaaaaaacag
     5281 gagggttgcg ctatgcacca gccttgacga agaaacagat aaagatggag tattatcgat
     5341 gccaagattg ttctgccacg atctgccgca agagaagaat cgatgtacgc aaatatcgtt
     5401 gtggaagatg tcatggagaa atcaaatgga ttgagacaaa aagagaagac tttaatgaag
     5461 cacaaaaaat cagatgaaag gatagaaggc ctttggaaaa aagtgagcag aggaatctaa
     5521 aagactggaa tcagctatta aattttttag ccgcacctga agagggaaat tttgaggaac
     5581 tgacggcacc tacacttatt ttagctggaa actgtcttcc tatcttagct gataaagcag
     5641 cacaaatgta tttaaatggc caagtagatc aattgttttt ggtcggaggg gtggggcatg
     5701 cgactagaat cctttatgag aattttgaaa agcaaggatt tcattttgaa gatggcatga
     5761 gtgaaagtga aatctgtcgt cagtatctaa aagaagtcta tgatttgccc gataaggctt
     5821 ttctgataga atcgaagtct acaaattctg gagagaacgc cactttttca ctggagattc
     5881 ttcattcctt aggagctgta ccagaaaaaa tcttattgat gaatgatccg acactgcaac
     5941 gcaggactcg agcaactttt gagaaagtat ggcaaaatga acaaactatt ttcgcgaatg
     6001 ctgtgccatt tgtgccggaa atccttcact tttctgaaga gatcatcttt acagcaaaag
     6061 aactgaatca tcaatggcct aaggaatatt tccatgcgct agtattagga gaaatggaac
     6121 gtctccatga cgatgagaac ggctatggtc cgaaaggaaa agattttatt cctcacattg
     6181 atatatcaga ggaagtttgg tcttcttaca ccagaattaa agagtcaata aaaacggatt
     6241 tttcccgtac ttgaccattt tagcaagcga atcctccttt ccaaataaat ttggttgaaa
     6301 ttttcaatcg tttataagat aatgaggagc gtgttgaatg taatagctaa aagaatggac
     6361 gctagataag agaagaggat tcaaatggaa aatcaaaata aattgtatca ttttgttggt
     6421 atcaaaggtt caggaatgag ctccttggct cttgttttac atgaacaagg tctaaatgtc
     6481 caaggatcag acattgaaaa atatttcttt acacaaagag atttagaaaa agcaaatatc
     6541 acgattctgc catttaacgc agataatgta aaaccaggga tgacgatcat tgcaggaaat
     6601 gcatttcctg attcacatga agaaatccag cgagcaaaag aattaggttt ggaagtgatt
     6661 cgctatcacg actttattgg tcattttatc caaaattata cgagtatcgc tgtaacaggc
     6721 tctcacggaa aaacaagtac gactggactg ctctctcatg tattgagtgg tgtccgtcca
     6781 acaagttatc taattggaga tggaacagga catggtgatc cgcaagcgga attcttttca
     6841 tttgaggcct gtgaatatcg ccgtcatttc cttgcctatt caccagacta tgcaatcatg
     6901 accaatatcg attttgatca tccggattat tatacaagta tcgacgatgt atttacagct
     6961 ttccaaacaa tggctggaca agtgaaaaaa gctatttttg cgtatggtga cgatgcttat
     7021 ttacgcaaat taaaagcaaa tgtgccgatt tattattatg gtgtcacaga aaacgatgat
     7081 atccaagcac gaaacatcga acgcacaact tctggttctg cttttgatgt ctatcatggg
     7141 gatgaatttg ttgggcactt cactgtacca gcattcggta aacacaatat cttaaatgct
     7201 ttaggtgtta ttgcagtagc ttatttcgaa aaactggatc tgaaagaagt tgcagaagaa
     7261 atgctgactt tcccaggagt aaaacgtcga ttcagtgaaa agatcgtagc agacatgacc
     7321 gtagtcgatg attatgcgca tcaccctgca gagatcaaag caacgattga tggcgcacgc
     7381 caaaaatatc cagataaaga aataattgca gttttccaac cccatacgtt tacgcgtacg
     7441 attgcattga tggatgaatt tgcagaagca cttgatttag ctgataaagt ttacttatgc
     7501 gatatttttg gttcggctcg ggaagaacaa ggaaacgtta agattgaaga ccttggagcg
     7561 aaaatcaaaa aaggtggaga agtcatcaaa gaaaataatg tatctccatt gttagattat
     7621 catgatgcag tagtaatctt catgggtgct ggagatgttc aaaaatttga acaagcgtat
     7681 gaaaaacttt taagcagcac gacgaaaaat gtactgtaat acaattactg cagtgtcctt
     7741 ataaaaatag aaagaggggg agtggcaaaa atcttgccaa gccccctttt ttgcaactta
     7801 tgataaacgg tgttcaaaag cttgcatctg cggtaataag cccaatcaaa tcaaaaacat
     7861 gagaagctgt ttttcttggc tgttcttatt acttgatgca gggcagcttt ttcacgacct
     7921 cagataaacg gtgttcaaaa gcttgcatct gcggtaataa gtccagccaa atgaaaaata
     7981 tgagaagctg ttttttcttg gctgttctta ttatttgatg caggacagct ttttcacgac
     8041 ctctgtaaac tataggaatc tgttaaatca agggagcaga acttgccttt tacgctactt
     8101 ttggtacaat aatgtatgat taaaaataaa ctggagggtt tctatcttga acataggtaa
     8161 gccgagaaaa ctaggaaaaa caataaatga aatcgaggaa ggcgactcgt tatccttaac
     8221 agaatctatt gaagataacc agcttttgct gtatttggga ttgacgaatg atgcaaatcc
     8281 tttgtatatc caacatgact ttgcacaaaa aacagaatat caacagccaa tcgttccatc
     8341 agtgatgctg atggggatca tcacaagtgc aatttcaaag catcttcctg gaccaggatc
     8401 acatgttgtc aatttttctg tcaattttat tgaaccggtt ttccattatg aaacactgac
     8461 ttttcagttt gaggtcataa aagtagataa gatgaaggat gtggtgacga tttctgtaga
     8521 agcggagaat acgaacgaag atcgtgtgtt ggatgctgtc gttatggtcc aaccgccaca
     8581 agttcctgaa ttggaaatag caatcgaaag tgaaggtgaa ttgaatgaat aatccaagta
     8641 catcaatgtc aggtatcaat cgtttttatg caaaagttta tggattttta ggtatgggac
     8701 ttgcccttag tgcagtcact tcttacttgg ctctttgggt atttcctttg caattagcaa
     8761 cttttatcaa taattttcca ctaggctttg tgggtctgtg gatcgttgaa ctgattttag
     8821 taatggttct aggcgtaaaa gcacaaaaga atccgagctt agcaatcggt ggatttattg
     8881 cctactctct tttgaatggc ataacattag cggttactct tgcatactac gatattggaa
     8941 cagtaacgca agctttcttg acagctactt tgatgttcat ggggatgtct gttgtcggag
     9001 taacaacaaa aagagatctt agcggggttg gaagagcagg acaggcagct ttaatcggag
     9061 tgatcattgc actagtcttg aacatcttca ttttgagaag tagcgtagtt gagctgttca
     9121 tctccctact gttagtcgtc atctttgcag ggattacagc ttatgataac caaagcatcc
     9181 gtaatatcta tgtccagaca aacggaacag cacatagtgg aattgctgta ttcatggcat
     9241 tgaagctgta tttggacttt atcaacttgt tcttggcgtt tctgagaatc tttggacgga
     9301 acaactaaaa aagaaaaaaa ttcatcagtt ggggctatac cgaattattt ggtatggtcc
     9361 cgctttttct tttcgcattc cgcaggattc tttgttagaa tgaataagaa ctttacagta
     9421 aggagttaag aagatgacaa aaaataaatt attactcgtt gatgggaaca gtgtggcttt
     9481 tcgcgcattc tttgcgcttc acaattctct tgaacgtttc aaaaatagaa acggtcttca
     9541 tacaaatgca atctatgcat tcaatacgat gtttgaaaat gttatgcaaa aagagcaacc
     9601 gactcatgtt ctggttgcgt tcgatgcagg aaaaacgact tttcggacag aaatgtatgc
     9661 ggagtacaaa ggtggaagat ccaaaacacc tggcgaattc aaagaacaaa tgccatatat
     9721 acgtgatttg ttgacaggac taggggtaca gtattacgaa ttgcccaatt atgaagcaga
     9781 tgatattatc ggtactttgg cagaaaaagt agacaaagat caatttgatg tcgtcgtgct
     9841 ttcaggtgat cgggatttga ctcaattagc tagtaaagaa gtaaaagtgg atatcactgt
     9901 aaaaggtgtc agcgatatcg aaagctatac accagaacat gtagcggaaa aatatgatgg
     9961 gttgacgcca aaacagatta ttgacatgaa gggactagcc ggagatgcct ctgacaatat
    10021 ccccggtgtg actaaaatcg gtgaaaaaac agctatcaag ctattgaaac aatatggctc
    10081 agtcgaagga atttatgaac atatcgatga aatgaagcaa agcaaaatga aagaaaatct
    10141 gataaatgac aaagagcagg ctttcttatc gaaaaaattg gcgaccatca atacttcagc
    10201 gcctgtggat gtctctatcg actcattgaa gtatgaaggg aaaaatctcg ataaactcgt
    10261 tccgttttat aaagaaatgg atttcaatca gttcctttct aaattgaata tcgtagaaga
    10321 agaagtaaaa atggatgata ttttgtttga agtcgttcat gaattcaaag aggaaatgtt
    10381 tactacggat atggcgttgt acgtggaaat gatgggcgac aattatcata cagaggaaat
    10441 cgtcggagta gcgtggggga cagaaaagaa aatttatgtc acgaacgatc tatctgtatt
    10501 cgaaaaccga gcgttccatt cttggatcac tgatccgact cggctgaaaa aagtatatga
    10561 tgcgaaacga acttatgttg ctttaaaccg ctatactgga aaaacgaaag gaatcgattt
    10621 tgatgtcttg ctaggtgcat accttctcga tacgaatgaa aaaagcacag atatcgaagg
    10681 aatagctgct cattacggat acaacgatat ccaatcagat gaagctgtat acggcaaagg
    10741 ggcgaaaaaa ggtttaccag aagaagagga actctttttt gctcatttag cgcgaaaagt
    10801 agctgcaatc aatgcgttga caccaaaatt gagccaagaa ctggcagata aaaaccaaga
    10861 agatcttttc cggaaaatgg aactgccatt gtctatcatt ttggcagaga tggaaataag
    10921 cgggatcaaa gtagatgcga ctcgcttaca agaaatgaag ggcgagttct ctgctcgctt
    10981 aagagagatc gaacaaaaaa tctacgaaga agccggcgaa gaattcaatt tgaattcacc
    11041 aaagcaatta ggagtgatct tatttgaaaa aatgggatta cccgttatca aaaaaacaaa
    11101 aactggatat tccacagccg tagatgtact ggaacagctt cgtgaacaag caccaatcgt
    11161 agaggatatc ctcacctatc gtcaaattgc aaaaattcaa tctacctatg tcgagggact
    11221 gctgaaagtg atccaatcag atggaaaagt gcatacccgt tatgtccaga cattgaccca
    11281 aacaggacga ttaagctctg ttgatcctaa tttgcagaac atcccgattc gattagatga
    11341 agggagaaag atacgtcagg catttgttcc tagagaaaaa gactggctga tttattcttc
    11401 tgactattca caaatcgagt tacgcgtact tgcgcatatc tcaaatgatg aacatttgaa
    11461 agctgccttc ctagaaggtc aagatatcca tgccagcaca gcgatgcgtg tatttggaat
    11521 cgaaaaggca gaggatgtca caccaaatat gcgtaggcag gcaaaagcag tgaattttgg
    11581 tatcgtgtat ggcatctctg attacggact ttcacaaaat ctaggaatca gccgaaaagc
    11641 agcacagcaa tacatcgata cgtactttga aaaataccca ggtgtcaaag aatatatgga
    11701 aagaatcgta cgcgaagcaa aagaccaagg ctacgtcgaa acgctgtatc accgccgtcg
    11761 atatctgcca gacatcaatt caagaaatta caatatccgt tcttttgcgg aacgaacagc
    11821 tatcaataca ccaatccaag gaagtgctgc tgatattttg aaaatcgcga tgatcgaatt
    11881 ggataaacga ttaaaagaaa caggtcttca agctacgatg ctgttacaag ttcatgatga
    11941 acttgtcttc gaggtaccgg aaaaagaact tgaatcatta gataaattgg tgaaggaagt
    12001 tatggaacaa gctgtttctc ttcatgtacc actgatcact gacagcagtt ggggaaaaac
    12061 gtggtacgaa gcaaaataaa cattgtttaa gacagagcaa tataatgaag gtgaaaagaa
    12121 aaaatgccag aattaccaga agtagaaacc gttcgtaaag gattggaacg gttagtagta
    12181 ggaaaaacga ttcaaaaagt tcaggtcttg tggcccaaga tcatcgagca accagagaca
    12241 cccatctttg aggcttctct tgttggcgaa accatccaat ccattggacg tagagggaag
    12301 tttttaattt ttcatctcga tcattgtgaa ctgatctcac acttgcgaat ggaaggaaaa
    12361 tatcaattta cgaaagaaaa tacaccaatc gataaacata cgcatgtact tttctttttt
    12421 gaggatggaa gccaacttcg ctacaacgat gtacgaaaat ttggtcgtat gacaatcgtc
    12481 gaaaaaggag caagtgcaac ttaccgagga atcatgaaat tgggacctga gcctttgcca
    12541 gatagcttct tattagcaga tttcgcaaat ggactgaaaa agtcccacaa agcaatcaag
    12601 ccattgctgc tagaccaacg tctagtgact ggtctaggta atatttatgt agatgaagca
    12661 ttatgggaag cgaagatcca tccagaacaa ccggcgaaca cactgcgccc caaagaggta
    12721 gaacaattac gtctatcgat cattgatgtt ttggatcgtg caatagaagc cggaggtacc
    12781 acgattcgca gttatttgaa tgcattagga gaatcaggcg gatttcaagt agcactgcat
    12841 gtctatcagc aaactggcaa accatgtata cgttgtggaa cgccgatcgt gaaaacaaaa
    12901 gtggcacaaa gggggacgca ctattgtccg aattgtcaaa ggctaaaaat cgcaaagtag
    12961 gatttgtttt aggtttgacg ggtggaatag ctaccggtaa aagtacggca gccaaagtct
    13021 ttcagtctca tggatttcct ttgatcgatg gcgatgtgat tgccagagaa actgttgagc
    13081 ctggaacgct tgctctgagg aaaattgtat cagtgtttgg ccaagaggta ttgcagcctg
    13141 atgggcaact agatcgaggg aaacttggga tgattgtttt tccatcaaag gaaaaacggc
    13201 aaaaacttga tcagctatta gatccgttta tccgaaaagc gataaaagat caaatcgcct
    13261 ctctaagttc caatcatcct ttagtaattg tagatatccc attgctctac gaaggtcatt
    13321 atgaccacta tatggatgca gtagctgtcg tttatactac gccagaaact cagcttcagc
    13381 gtttgatgaa acgtaatcaa ctgacgctag aacaagcgca gcagagaatt agtagtcaac
    13441 ttccaatcga agaaaaaaag cagcgtgcgg atattttgtt cgacaacaat ggaacaaaag
    13501 aaaaacttgt gggacaaatc gaaaattggc tttttgctaa tcgctttctt tgagtatctg
    13561 tgtcaatttt gctatcctga gtatatagaa agtgagggat agcgatggga tcattaaaaa
    13621 atcaaaagga taaagtcgta ggtaacgcaa aagaaaaaat cggcgagatg ctcaataaag
    13681 aaaatctaat cgatgaaggt cgtacgcaat cagaaacagc agaagaaaaa gaaaagcagt
    13741 accaagcaag aatcgatgaa atagaagacc agcaggcaga agaagaagcg aagcggcaag
    13801 atttgactca agaacgaaaa aatccgacac ctgatcgtag taaaacaccg ctcccagaaa
    13861 gtgaccacat ttccgatgag caaaaaatcg aggaacagcg aatgaaacac tatacaggaa
    13921 tcgaagagaa gttataaaat agtaaaaaat cgagatgtga cttgaagcag atgagaaggt
    13981 gttcatctgc ttcaagtttt ttgtgctaga tgactaaatc agcttttgtg taagttattt
    14041 ctcagtaatc aaggtgatca gtattggaaa attgaaattt agtaaggaca ggggacacaa
    14101 ttacgcatga aagtagaaaa aattgttctt atatccgtgt tttttgggta ggacagtcgc
    14161 gtttgtgatt tgtttcgtgt tataatacaa ctagttgtaa agaaagacac atcctttata
    14221 taaaatgcga tgcaagaggt gagacaaaat gagatgtccc agatgtcaac ataataattc
    14281 acgagtaatc gacagccgtc aagcagatga tggacgtgcg atccgtcgcc gtcgcgaatg
    14341 tgagaattgt tcgttccgat tcactacatt tgaacgaatt gaagctgctc cgttattagt
    14401 aatcaagaaa aacggggaac gcgaagagtt caatcgcgat aaaattttaa gaggactcat
    14461 ccgttcagca gaaaaaagac ctgtagctat ggaacaaatg gaacaaatcg tcaaccatgt
    14521 cgaaaaccgc gtacgcagtc tcggagaaaa cgaagtatcg acgaatctaa ttggtgaata
    14581 tgtgatggaa gatttagttg atttagacga aatcgcttat attcgttttg caagtgttta
    14641 tcgccagttc aaagatatgt ctgttttctt aaaagagttg caagatatcg tggataaagc
    14701 aaaagaaaat aataaagaga ataataaaga aaacaattaa gaaacaaaaa ggaggcattt
    14761 ccttggaaaa tgcttggaaa gagcttcaac caaaaaatat ttaccaagga agaaaagcgg
    14821 ctccgctgag agaagacagc aagaacgcct tgctgtatct ttatcagcca ctaatcggcg
    14881 gagaagcctt gagtatctat ttcaccctgt tatcagaaat ctctcaagaa tctggcaaag
    14941 gtcctgaagg gatgcatgca gatctgctta gtagtctagg ctgcggtatc ccgcaatttt
    15001 atgatgcaag aaaaaagcta gaaggaatcg gcttactgga tgtttatttt aaagaagata
    15061 gtgatctggg acctcgtttt atttatgagc tgttagaacc actcattcct aaagcatttt
    15121 tcaaagactc tgttctggct tttcttcttc tggaaaaagt tggtgaacga cgattccaga
    15181 agcttaccca attctttgaa cctaaaaggc atacagcaga gggatatcaa aaaatcacaa
    15241 aaaaattttt agatgtttat cgcttcaatg aagcagtgta cgctgctaat caagaaaaca
    15301 tcgaatcgct gaataaatct tttgaagaat ctgaatcagt agcattactt gaagcagatt
    15361 cgacattcga ttggcagttt ttgaatgatt ggctggcaaa aaaacatatt gcaagtcttg
    15421 atgatccaat catcaaacaa gtcgacatgt atcatcagct ttacggtttc gatgaattga
    15481 cacttggcga actgatcagt caatcttatg actttagtga acaaaaagta tctgaaaaag
    15541 aactgcaaaa agccattttg gcaatgcaga gacgtccggt gaagcaaaca aaagaaacag
    15601 ttgcggtttc tgaaaacagt aatgaaccct cgtcaaacga acatttgaca aacgcagcgc
    15661 ttcagctgat aaaagaagca cagagcattc caccgatgaa atatctggaa gcaatcaagt
    15721 cagaaaaagg cggatatgtt tctaaacaag aaaactggtt actgcaagat cttgtttctc
    15781 aatcaggtct ttccagtagc gtgatcaatg ttttgctgaa ttatgtacta gtgatcaaga
    15841 ataatgctag cttaaatgcc agttatgtaa atacagtggc aaatgaatgg gcacaaaaga
    15901 aaatcgccac agcagaagaa gcgatccagc acattcggca aataagtaat caagcaaaac
    15961 aatcaaaaca aaagaagcaa acaaattatc aaactggcaa gcgaaatgtt cgacgggaaa
    16021 agcttcctga ttgggtcaac cagccaaaag atgaaaaaca gatcagcagc gagaaaaaag
    16081 cagagattga ccgccgtttt aaagaatacc tggcaaaaaa ggagggtgat atctagtgga
    16141 agacgtaggc aaagaattga agaggatcat cagcaaacgc aattatcaag aaaag
//
LOCUS       DACEEZ010000066        15687 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20313_2017
            SAMN12639966-rid9879223.denovo.066, whole genome shotgun sequence.
ACCESSION   DACEEZ010000066 DACEEZ010000000
VERSION     DACEEZ010000066.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639966
            Sequence Read Archive: SRR10023158
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 15687)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15687)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741683.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741683.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:44:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,016
            CDSs (total)                      :: 2,962
            Genes (coding)                    :: 2,889
            CDSs (with protein)               :: 2,889
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 36 of 73
            Pseudo Genes (incomplete)         :: 29 of 73
            Pseudo Genes (internal stop)      :: 18 of 73
            Pseudo Genes (multiple problems)  :: 8 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15687
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639966-rid9879223.denovo.066"
                     /isolate="E20313_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            68..751
                     /locus_tag="IWO89_002075"
     CDS             68..751
                     /locus_tag="IWO89_002075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnogalacturonan acetylesterase"
                     /protein_id="HAQ0456801.1"
                     /translation="MPTIHIAGDSTAAEKDENKRPETGWGEKISAYFSPEIQINNQAK
                     NGRSTKSFIQEGRLARLASQFQPNDFLFVQFGHNDQKIQEERGTLPFGSYLKNLQIFF
                     ECAKKANVQMVLLTSVTRRDYLENGTLNPDILGDYPKAMRAFAEKHHIPLLDVFSRSQ
                     ELFQTFSKEETKKFYLHLMPDTCKNYPEGLKDNTHFSPEGADKVARIIVELIKESRLP
                     LANYLQKGV"
     gene            753..1589
                     /locus_tag="IWO89_002076"
     CDS             753..1589
                     /locus_tag="IWO89_002076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HAQ0456802.1"
                     /translation="MFPLNLGIRAHDLDVRNREEIVQKVSQLHLSHIQFAPQKAFPEQ
                     LKLTDMTLGSAAYFGEYFQKNNIELSILGCYINLSSKNQEVRAQALATFKQQIALCSA
                     YQAKMIATETGSVSVGYTKENFTEEAYQIARNSIIELVAHAENFGVTVAVEAGINHPI
                     YNYQLAKRLIDEVASPNLKIILDCANLMHKENHADQEKVIRGALDNLHGYITALHLKD
                     YIMENGKIRMVPVGKGCLDFRPVLHYIKYERPLMYATLEATPEIYVPEAIEHLQFIYQ
                     DC"
     gene            1600..3354
                     /locus_tag="IWO89_002077"
     CDS             1600..3354
                     /locus_tag="IWO89_002077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ0456803.1"
                     /translation="MEERIQKSYQKYHANAFQTLLHLYQGNYWRFAMSGLFFIIKHLP
                     SWLMPIAIANVINAASSKDSSQLQAIYFNAFLMACLIGQNILTNYFHVKYHSTSIRQV
                     EAGLRATLIKKIQELSIPYQKELQSGRIQSKIIRDVEAIQTLSSQVFVSGLNIAVNLF
                     VALGITLYKSPVVFMFFLLTVPLAAFVTLFFRKRIRQSNSDFRQEMEETSAKVIEVIE
                     LAPIARAHGLEKHETTRLNKHFTQIYEKGFHLDILQSVFGSVSWASFQIFQLICLIFT
                     GILALQGRIQPGDVVMYQTYFTTIVNSVSGMITLIPTISKGMESITSIGELLIAGDVE
                     TYHGKKKIQHLDGNFRFEDVSFLYPDSLKPIIDHFSLEVKAGETIAFVGPSGSGKSTL
                     LNLLIGFIQPTSGKIFLDDQPLDELDMRSVRNYLAVVPQTTLLFSASIKENITYGLKN
                     VSKERLDEVIEAAQLSSLIDQLPDGLDTLVGEHGNKLSGGQKQCISIARALIRQPKII
                     LLDEATSALDNQSEKKIQQALNYLTETPTTFIVAHRLSTIKEADKIVVIDEGKIVEIG
                     TYEKLLAQKGYFYRLKNE"
     gene            complement(3630..3971)
                     /locus_tag="IWO89_002078"
     CDS             complement(3630..3971)
                     /locus_tag="IWO89_002078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbF family regulator"
                     /protein_id="HAQ0456804.1"
                     /translation="MSNIYDSANQIEREIRELPEFKALKEAYEKVQANEEAHKLFKDF
                     QAMQIELQEKQMSGQEFSDEDAMKAQEMAVKIQSEEVINDLMQKEQGFSTIINDLNRI
                     IMTPVRDLYSE"
     gene            complement(4039..6210)
                     /locus_tag="IWO89_002079"
     CDS             complement(4039..6210)
                     /locus_tag="IWO89_002079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PBP1A family penicillin-binding protein"
                     /protein_id="HAQ0456805.1"
                     /translation="MDFQSILEKIKAALISFWRWIRPYLGQFHRWRKRIWKKYHVNKL
                     ILLIGLICVLVTSIYLFILAKQANVETLKSGLSQSTVIYDKKNEEAGTLYAQKGTYVE
                     LNAISPYIQDAVISTEDRNFYEHHGFDIKGIARAAVRMVLSGGTSGGGGSTITQQLAK
                     NAYLTLDQTFDRKAKELFLAIEIEKKYSKEEILTMYLNNAYFGNGVWGVQDASRKYFG
                     VDATNVTLSEAATLAGMLKGPGIYNPIDHPKNANDRKNTVLSVMEENGKITKEQEQAE
                     SQTDISAYLNDTYDNSDSGYRYPYYFDAVIDEAVNEYGLDEEDVMNKGYKIYTALDQD
                     YQKQLEATYQNSALFPANASDGAMVQSASVALDPNTGGVQALVGRRGDHVFRGFSFAT
                     QMRRSPGSTIKPLSVYTPALEAGYKPSSILKDEPQSYYDAHNFDGTYQGEVPMYQAVA
                     QSLNLPAVWLLHEIGLQKGYDKAEEFGLPLAKSDKYYGLALGGLEKGVSPLIMAGAYS
                     AFANDGQMYTPHLITKIVDSTGAVIVDNTNKKPKQVISKETADEMTSMLLGTFSNGTA
                     AAANPAGYTIAGKTGTTETNFDATKANDQWMIGYTPDVVISTWMGYEESSKLHYLEGT
                     SGNVVGKVFKSAAEGILPYTSKTGFSVADAYATGGQVVAADQVGNTGNSNGESGNWQD
                     NLNQYGEQAKEGLKNFGDMVKEGVQGIGEAAKDLWRKYQGE"
     gene            6421..7278
                     /locus_tag="IWO89_002080"
     CDS             6421..7278
                     /locus_tag="IWO89_002080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HAQ0456806.1"
                     /translation="MKITITLPMNHPETTIRDLLEKEWLVPRKVRHFLRIRKNVWINQ
                     QPALFHHEVKGNDQITLLFEETDYNYQEVQLGDAKHIRVLYEDEHLIIVDKPAGMKTH
                     PNEPTENSTLLNHLAAYLDTKQQRPYVVHRLDKETSGAVLFAKNPFVLPILGRMLEQK
                     MIYRRYQAIVWGEIAKDMTITNKIGRDRHDRRKRVVDDKNGQSALTHVTVNKVLSEQT
                     QVYCVLETGRTHQIRVHLAHKGHPIVGDPLYQNRTAERLMLHALELHMIHPFTQEKIV
                     GKALPGLWE"
     gene            complement(7349..8206)
                     /locus_tag="IWO89_002081"
     CDS             complement(7349..8206)
                     /locus_tag="IWO89_002081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HAQ0456807.1"
                     /translation="MYGGIEAGGTKFVCAVSDGGEILEKLSIPTTTPEETLDQVFEFF
                     DRYELKGIGIGSFGPIGIDKTKDNYGYVLATPKKGWAQFDFLGTIKERYNIPAAWTTD
                     VNAAAYGELKQGAAQGKNSCIYLTVGTGIGAGVVINEEIFSGIAHPEMGHIWVKRHPD
                     DHYEGTCPYHKDCLEGLAAGPSIEARTGIKGQDLPQDHPVWAIQAYYIAQALINYTLT
                     LAPEKIILGGGVMNQDHLLQKIRQQFVELMGGYMETPQVSEYIVRWGLPNESGIIGSL
                     LLAEHEHQK"
     gene            complement(8191..10893)
                     /locus_tag="IWO89_002082"
     CDS             complement(8191..10893)
                     /locus_tag="IWO89_002082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-mannosidase"
                     /protein_id="HAQ0456808.1"
                     /translation="MKKKVYIISHSHWDREWYMAYEQHHMRLIELIDDLLELFEVDPS
                     FNSFHLDGQTIILDDYLQVRPEKRQAIQQAINEGKLRIGPFYILQDDFLISSESNVRN
                     MLIGMEESRKWGTPVMLGYFPDTFGNMGQTPQLMKQAGISAAAFGRGVKPIGFDNQVL
                     EAENYSSQYSEMWWKGPDQTAIFGLLFANWYSNGNEIPVEKEAALAFWKQKLADAEQY
                     ASTNHLLMMNGVDHQPVQKDISKAIHLANELFPDYEFIHSNFTDYLEAVQKDVPEDLG
                     SVEGELTSQETDGWYTLANTASARVYLKQWNTKVQRQLENITEPLATMAYEVSGNYPH
                     DQLDYAWKTLMQNHPHDSICGCSVDSVHREMIPRFEKADEVGKYLAQDSLEQLTAAID
                     TTGFPKDSFPFVIVNTAGMDKTGEAEITIELERKRFAEGIPEQLYQELENLPKRKYHV
                     ETKSGATIPAILSEETVQFGYDLPKDRFRVPYMARMIKVTLPLENMPAFSWETFALVE
                     GEAKAEEKETMIHQSGRIIENGPLHLTIEKNGTITMEDRKNKRKLNNLHIFEDIGDIG
                     NEYIFKQPFCDQPILSSNKENSEVKVLVDTPEIAKISILQEMEIPVSADERLEKEQQM
                     VVEFRYRKAERSKEKRILQIKTIMTIRKDSKKIDFETTLDNQMKDHRLRVLFPTKLHV
                     EHHEADSIFEVVKRPNHVSKSWENPTNPQHQQAFVNIHDEEYGVTVGNFGLNEYEVTE
                     DGQIAVTLLRSVGELGDWGYFPTPEAQCLGEHRFNYSIELHGPEEKFSTYLHAYAAQI
                     PFSTQQIKHHEGTLISKQQYLTIKSETFAITALKRSKFSDKVVVRGFNMSSHLEKLEI
                     TKDNGKTVILNLLEEPTKQAVVPIIQPYEIRTIGFEEENECMAE"
     gene            complement(10906..12195)
                     /locus_tag="IWO89_002083"
     CDS             complement(10906..12195)
                     /locus_tag="IWO89_002083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 125 protein"
                     /protein_id="HAQ0456809.1"
                     /translation="MVYKDIPKSVQKFMEDITQKCGDIHADWAKNFTAAFANTLLTTV
                     KKQNDGTTFLLTGDIPAMWLRDSTAQVRPYLVIAKEDEELADMISGLVKKQFFYINID
                     PYANAFNEEANGAGHQTDHTKMNDWIWERKYEIDSLCYPIQLAYLLYKNTGKTDQFNQ
                     SFIEGIKKILDVFKTEQDHEHSPYTFVRDTTRKEDTLINDGRGAKTAFTGMTWSGFRP
                     SDDACRYGYLVPSNMFAVVVLGYVEEIFQTILDDKEIVQQTQKLKKEIQEGIQNHGRT
                     VNQKGQTIYAYEVDGMGNATVMDDSNVPNLIAAPYLGYCSEQEEDYLQTRRTLLSKEN
                     PYFYEGKYAKGIGSSHTPEKYVWPIALAMEGMTTSDKSEKERILNLLVATDAGTHLMH
                     EGFDVNNPENYTREWFSWANMMFCELVMDYFDIRVEK"
     gene            12345..13391
                     /locus_tag="IWO89_002084"
     CDS             12345..13391
                     /locus_tag="IWO89_002084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAQ0456810.1"
                     /translation="MTQPLYQTIFNDLQTAILTGELPVDSQLPTEKELSRTYKVSRIT
                     SKRALTELEQLGLIYRVRGKGSFVKSASPEQILASPTKTRRILFLLPYVADLSVGDFS
                     KGLNPVMQDKQIDVMMTTLDFLQNKTAKELINEFDGLIYYALDTEQHLDLLFELSLKE
                     FPVVILDKKIYELPFPTVLSDNFQGGALAASYLIEQGHSKIAYLFGTQTHPQSVRQRY
                     LGYLEALDKAGLTFHTSLDDKSATLDELLSYITKYEVTAFVCENDVVAIQTMNLLRQH
                     GYHIPEDFSIIGFDDIQAAALVDPPLTTIAQDFEQLGKLAGDALVTWLETKDRPSDSK
                     FPVSFIKRQSTKEK"
     gene            13393..15546
                     /locus_tag="IWO89_002085"
     CDS             13393..15546
                     /locus_tag="IWO89_002085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-mannosidase"
                     /protein_id="HAQ0456811.1"
                     /translation="MNIGQIDTRHATANQPNFSNGNCQPYTGVPFGMNYFAPQTTDQN
                     GSWWFHPDDRVFQGYRLTHQPSPWMGDFSHMLFTPINGKLQENTLFHAQSSYRPEESI
                     FCPTHLSICQLRYGICSTLIPSMYGGILSIDYSKNDSGLLLSFPGRHQLFVIDPYTVE
                     GKITHFAGSEDPDFSFYFILRFEYPLTIDELAVSNKNSDSIPLYFEQTKKQTIRFGTS
                     FISSEQAAYNLELEQNQSKETYLAHSQEQWQSYFDRIQLTHHDQQQLRTFYHNLYRVF
                     LFPQTFYEIDPSGQKIHYDTTSRSIKPGVLYTNNGFWDTYKTVYPLYSLIAVEKYEEM
                     LEGFLNSYRESGYLPKWLSPDERGLMPGTLIDAVIADAAVKDIRRDLMPEFLTAMEKG
                     ATVQSKHPNYGRQGTEDYLTYGYVPNHYHESVNHTLDYAYSDFCISQVAKVLEEEEMT
                     KKYQQQSLNYQNIFDYESGFMRSKDKDGKFREPFSANRWGQDYAEGSAWQSSFAVYHD
                     FSGLIGKYGGSERYKEKLIELCNQPPTFDPGGYGFEIHEMSEMAAIEFGQLAISNQPS
                     FHYPYLFSYIGQPEMAQPLLKQLMTQTFDDSPMGYPGDEDNGSMAAWYIFNSLGFYPV
                     TPGTGEYVIGMPLIDKAVLLLSNGKELTIEATPNRPQQQFIHKIKRMDREYTKLYFTH
                     QDLLKGGTISYQLGIIPHPKKYQAKDLPFSLSNYN"
BASE COUNT         4454 a         3282 c         2887 g         5064 t
ORIGIN      
        1 ttgctccgtc ttcttctctt gctgttctgc ttcattgcct ataaaccttt atgaaagaag
       61 agataacatg cctactatcc atattgccgg tgattctact gcggcagaaa aagatgaaaa
      121 caagcgtccc gagacaggtt ggggagaaaa aatctctgct tatttttcac ccgagatcca
      181 gatcaataac caagcaaaga atggacgcag caccaaatca tttatccagg aaggaaggtt
      241 agcccgtcta gcttcacagt ttcagccgaa tgatttctta tttgtacaat ttggacataa
      301 cgaccagaaa atccaagaag aaagaggtac gcttcccttc ggtagctatt taaaaaatct
      361 gcagatattt tttgaatgcg caaaaaaagc gaacgtccaa atggtcttac tgacatctgt
      421 gacgcgacga gattacttag aaaacggaac attgaatcca gatatacttg gagactatcc
      481 aaaagcgatg cgtgcatttg ctgagaaaca ccatattcct cttttagatg tgttctcaag
      541 atcacaagaa ttgttccaga ccttctcaaa agaagagaca aaaaaattct acctgcatct
      601 tatgcctgat acttgtaaaa attacccaga aggactaaaa gacaataccc actttagtcc
      661 agaaggcgct gataaagttg cccgaataat tgttgaattg ataaaagaat cccgtctgcc
      721 tcttgctaat tatttacaaa aaggagtttg agatgtttcc attaaattta ggcatccgtg
      781 cccacgattt agatgttcga aatcgtgaag aaatcgttca aaaagtatca cagcttcact
      841 tgtcccatat ccaatttgca ccacaaaaag catttcctga acaacttaaa ttaactgaca
      901 tgacacttgg gagtgctgcc tattttggtg aatacttcca aaaaaacaat atcgaacttt
      961 ccattttagg atgctatatc aacctatcca gcaaaaatca agaagttcgt gcacaagcat
     1021 tggcaacttt caaacagcaa attgctctgt gctctgccta tcaagccaag atgattgcta
     1081 cggaaaccgg tagtgtctct gtcggttaca caaaagaaaa ttttactgaa gaagcttatc
     1141 agatcgccag aaattctatc attgaattag tcgctcatgc agaaaatttt ggcgtaactg
     1201 ttgctgttga agcagggatc aatcatccaa tctataacta tcagttagct aaacgtttaa
     1261 tcgatgaagt agcttcacca aatttaaaaa tcattttaga ttgtgcgaat ttgatgcata
     1321 aagaaaatca tgctgatcag gaaaaagtga tccgaggtgc cttggacaat ttacatggtt
     1381 atattacagc gcttcatctc aaagactata tcatggaaaa tggaaagatc cgcatggtac
     1441 ctgttggcaa gggctgttta gatttccgcc cggtactgca ttatatcaaa tatgagcgtc
     1501 ctctaatgta cgccactctt gaagctactc ctgagattta tgttcctgaa gcaatagaac
     1561 acttgcagtt tatttaccaa gattgttaag gagaagatta tggaggaacg aatacagaaa
     1621 agttatcaaa aatatcatgc aaatgctttt cagacattgc tgcatcttta tcagggaaat
     1681 tattggcgtt ttgccatgtc aggactcttt tttattatca aacatcttcc atcttggtta
     1741 atgccgatcg ctattgccaa tgtaatcaat gcagcttcat cgaaagattc ttctcagttg
     1801 caggccattt atttcaacgc ttttttgatg gcttgtctga ttggtcaaaa tatattgacg
     1861 aattattttc atgtaaaata ccatagcact tcgatccgtc aagtagaagc tggactaaga
     1921 gcgaccctta tcaagaaaat ccaagaacta tctattcctt atcaaaaaga attgcaatct
     1981 ggacgaatcc agtcaaagat catccgtgat gtagaagcta tccaaacgtt gtcctctcaa
     2041 gtatttgtca gcggattaaa tatcgctgtc aatttattcg tagctttagg tatcacgctt
     2101 tataaaagtc ctgttgtttt catgtttttc ttattgactg ttcctctcgc cgcattcgtt
     2161 actctctttt tccgtaaaag gatcagacag tccaactcag acttcaggca agaaatggaa
     2221 gagacttctg caaaagtgat cgaagtgatc gaattagcac cgattgctcg agctcatggg
     2281 ttagaaaaac acgaaacaac tcgcttaaat aagcatttta ctcaaatcta tgaaaaagga
     2341 ttccacttag atatcctgca atctgttttc ggatcagtca gctgggctag ttttcagata
     2401 tttcagctga tttgtttgat tttcactggg atattagctt tacaagggcg catccaaccc
     2461 ggcgatgtcg tgatgtatca gacgtatttc acgacgatcg tcaattccgt ttctggaatg
     2521 atcacgctga ttcctacgat ttcaaaaggc atggaatcta tcacttctat tggcgaactg
     2581 ttgattgctg gcgatgtaga aacttatcac ggtaagaaaa aaatccaaca tttggatgga
     2641 aattttcgtt ttgaggatgt ttcttttctt tatccggact cactcaaacc gattatcgat
     2701 catttttctc tagaggtgaa agctggagaa acaattgctt ttgttgggcc atctggctct
     2761 ggaaaatcta cactccttaa cttattgatt ggatttatcc agccgacttc aggaaaaatt
     2821 tttctagatg atcagccttt ggatgaactg gatatgcgaa gtgtgcgaaa ttatttagcc
     2881 gttgttcctc aaaccacttt gttgttctcc gcttcgatca aagagaacat cacttatggc
     2941 ttgaaaaacg tatcaaaaga acgtttagat gaagtcatcg aagctgcgca actttcttca
     3001 ctgattgacc agcttcctga tggtctggat accttagtag gcgaacatgg caacaagctg
     3061 tctggtggcc aaaagcaatg tatctccatt gcacgagcgt tgatacgtca gccaaaaatc
     3121 attttgctag atgaagcaac ctcagcttta gataaccagt cagagaaaaa aatccagcaa
     3181 gccctgaatt acctgacaga aacacccact acttttattg tggctcatcg gctttcaacg
     3241 atcaaagagg cagataaaat cgttgtgatc gatgaaggaa aaatcgttga aataggtact
     3301 tacgaaaaac tgttagctca aaaaggctat ttctaccgct tgaaaaatga atgacaaaaa
     3361 ataggagcta tgacaagact tttgtcgtag ctcctatttc tgaataaacg gtgttctcct
     3421 gccaactcct cggtcacaag tcacaatagt catcaaacat gagaagctgt tttttgaaat
     3481 tgttccttgt aactcagagt taaacggtag gctcacgacc tcttggtaaa tggtgttcaa
     3541 gagacagcct atcggcaata agaagcaatt ttgtcatcgc tcaaggccga acgtctcttt
     3601 cacaccctct gttgtttcat aaaatagttt tattcgctgt aaaggtcgcg aactggtgtc
     3661 atgatgatac ggttcaagtc attgatgatt gtactgaagc cttgctcttt ttgcatcaag
     3721 tcattgatta cttcttctga ttggattttc acagccattt cttgtgcttt cattgcatct
     3781 tcgtcgctga actcttgtcc actcatttgt ttttcttgta attcgatttg catcgcttgg
     3841 aagtctttga acagtttgtg tgcttcttcg tttgcttgta ctttctcgta cgcttctttc
     3901 aacgctttga attctggtaa ttcacggatc tcacgttcga tttgattcgc gctatcgtag
     3961 atgtttgaca tgttatgccc tcttttcttt tagtagttac tctctattgt aacatggggt
     4021 tattcgtttt caaaggggct actctccttg atatttccgc cagaggtctt tagccgcttc
     4081 accaattccc tgaacgcctt ctttcaccat atcgccgaag tttttcagcc cttcttttgc
     4141 ttgttcacca tattgattca agttatcttg ccaatttcca gactctccat tagaatttcc
     4201 tgtgtttcca acttgatctg cagctaccac ttgtcctcct gtggcatagg catctgctac
     4261 actaaatcct gtttttgaag tatacggaag gatgccttct gccgctgatt tgaagacttt
     4321 cccgacaaca tttccacttg tcccttccaa atagtgcagc ttgctggatt cttcgtaacc
     4381 catccatgtc gaaatcacta cgtctggcgt gtaaccgatc atccattggt catttgcttt
     4441 tgtggcatcg aaattagttt ctgtggttcc tgttttccct gctatcgtat aacctgcagg
     4501 attcgcagca gccgctgttc catttgaaaa tgtaccaaga agcatgctgg tcatttcatc
     4561 ggctgtttcc tttgatatga cctgttttgg ttttttgttt gtgttatcga cgatcaccgc
     4621 tccagtcgaa tcaacgattt tcgtgataag atgcggcgta tacatctgac catcattggc
     4681 aaatgcactg taagcacccg ccatgatcaa tggagatact cctttttcca gtccaccaag
     4741 ggctaagcca taatacttat cagattttgc taaaggcagg ccgaattctt ctgctttatc
     4801 atatcctttt tgtaaaccga tctcatgcaa aagccataca gcaggcaaat tcaaactttg
     4861 tgcaacagct tgatacatcg gtacttctcc ttggtatgtc ccgtcaaaat tatgcgcatc
     4921 gtaataactt tgcggttcgt ctttcaagat actggaaggc ttgtatccag cttccagtgc
     4981 tggtgtatat acgcttaatg gtttgatcgt tgatcctggt gaacgacgca tctgtgtagc
     5041 aaagctgaat cctctaaaga catgatctcc tcttcgtcct actagtgctt gtacaccacc
     5101 tgtattcgga tctaaagcta ctgaagcaga ttggaccatg gcaccatctg aagcatttgc
     5161 tgggaataat gcactgtttt gataagtagc ttctaattgc ttttgataat cctgatctaa
     5221 agctgtatag attttatagc ctttattcat gacatcttct tcatcaagac catactcgtt
     5281 aactgcttca tcgatcactg catcaaaata ataaggataa cgataaccag aatctgaatt
     5341 atcgtatgtg tcattaagat acgcgctgat atctgtctgg ctttctgctt gttcttgttc
     5401 ttttgtgatt tttccgtttt cttccattac acttagtact gtgtttttcc gatcattcgc
     5461 attttttggg tgatcgatcg gattataaat accaggacct ttcagcattc ctgccaaagt
     5521 agcagcctca gacagcgtaa cattcgttgc atctactcca aaatactttc tggaagcgtc
     5581 ttggacaccc cataccccgt tgccaaaata ggcattgtta agatacatgg tcaagatttc
     5641 ttctttgctg tacttctttt cgatctcgat tgccaaaaat aattccttcg cttttcgatc
     5701 aaacgtttgg tctaatgtca gatacgcatt tttggcaagc tgctgagtga tcgtacttcc
     5761 tccgccccct gatgttccgc cagacaagac cattcggact gctgcacggg caatcccttt
     5821 gatatcaaac ccgtgatgct cgtaaaaatt cctatcttct gtcgagatta ctgcatcttg
     5881 gatataggga gaaatcgcgt ttaattctac atatgttcct ttttgggcgt acagtgtgcc
     5941 agcttcttcg tttttcttat cataaatgac tgtcgattgg cttaatcctg attttaacgt
     6001 ttcgacattt gcttgttttg ctaaaataaa gaggtagata cttgtcacta agacacagat
     6061 caaaccaatc agcaaaatca atttgttcac atgatatttt ttccagatgc gctttcgcca
     6121 tcgatggaat tgtccgagat atggtctgat ccaacgccaa aaactgatca aagcagcttt
     6181 tatcttttct aaaatgcttt gaaaatccat ttgcttcttc tcctgtctat ttgattcaat
     6241 cgtcttattt taccatagtg aaagagtgtt ttcttcctgc ttaagttgta gttcaatatg
     6301 tttttttacg ttagtgcaag tagagtaaca taagaatgaa cagaaaaatg taagtttttt
     6361 cttaaatatg ttttaaaaat ggtacaatta gtctgcaatt gcactgaaag gagtacactt
     6421 gtgaaaatca cgatcacttt acctatgaac catcctgaaa caacaattcg cgacttgctt
     6481 gaaaaggaat ggttagttcc tagaaaggtt cgacactttt tgcgtatacg taaaaacgtt
     6541 tggatcaatc aacaacctgc tttgttccat catgaagtaa agggcaatga ccagatcaca
     6601 ttgttattcg aagaaacaga ttataattat caagaagttc aattaggaga tgcgaagcac
     6661 atccgtgttt tatatgaaga tgagcatttg attatagtgg ataagcctgc aggaatgaag
     6721 actcatccaa atgaaccgac agaaaacagt accttactca atcatttagc tgcttatctc
     6781 gacactaaac agcaacgacc ttacgttgtc caccgcttgg acaaagaaac aagcggtgcc
     6841 gttttatttg caaaaaatcc atttgtcttg ccgatacttg gaagaatgtt ggaacaaaaa
     6901 atgatctatc ggagatatca agccatcgta tggggagaaa ttgccaaaga tatgaccatc
     6961 accaataaaa ttggacgaga tcgccatgat cggagaaaaa gggtcgtaga tgataaaaat
     7021 ggtcagagcg ccttgacaca tgtcactgtc aacaaagtac tttctgaaca aacacaagtc
     7081 tattgtgttt tggaaaccgg acgtacacac cagatcagag ttcatttagc tcataaggga
     7141 catccgatcg ttggagaccc tctctaccaa aatagaacag ctgaacgact gatgctccat
     7201 gcattagaac tgcacatgat ccatccattt actcaagaaa aaatagtcgg taaagcttta
     7261 cctggattat gggaatagaa ataaaaaaga acagagtctt atcagcagat aatgataaga
     7321 ctctgttctt tttgtgtaat ttgtctgctt acttttgatg ttcgtgttct gctaacagca
     7381 aactaccgat aatcccactt tcattcggca gtccccaacg aacgatatat tcacttactt
     7441 gaggtgtttc catataacca cccatcaatt caacaaattg ctgacggatt ttttgaagga
     7501 gatgatcctg attcattacc ccaccgccta aaataatctt ttcaggtgct aacgtcaacg
     7561 tatagttgat cagtgcttga gcaatataat aagcctgaat agcccaaaca ggatgatctt
     7621 gcggcagatc ttgccctttg atccctgtgc gtgcctcaat agacggtcct gctgctaatc
     7681 cttctaaaca atctttatga taaggacaag taccttcata atggtcatct ggatggcgtt
     7741 tcacccaaat atgtcccatt tcaggatgtg cgatccctga aaaaatttct tcattgatca
     7801 cgacacctgc accgattcct gttcccacgg taaggtaaat acaactattt tttccttgtg
     7861 ctgctccttg cttcaattcc ccatatgcag cagcattgac atccgttgtc caagcagcag
     7921 gaatattgta gcgttccttg attgtgccaa gaaaatcgaa ttgtgcccac ccttttttgg
     7981 gagttgccaa aacgtatccg taattatctt ttgttttgtc gataccaatc gggccgaatg
     8041 agccaatccc gattcccttc aattcataac gatcaaaaaa ttcaaatacc tgatccaatg
     8101 tttcttctgg tgttgtggta gggatactta atttttctaa aatttctcca ccatctgaaa
     8161 ccgcacacac aaattttgtt ccgcctgctt ctattccgcc atacattcgt tttcctcctc
     8221 gaatccgatt gtccgaatct cataaggttg gattattggg acaaccgctt gcttcgttgg
     8281 ttcttcaagc agattcaata tgacagtttt tccattgtct ttagttattt ccaatttttc
     8341 taaatgacta gacatattga aacctcttac tactacttta tcactaaact tgctgcgttt
     8401 caaagctgta attgcaaacg tttcactttt tatcgttaaa tattgttgtt ttgaaatcaa
     8461 tgttccttca tgatgtttga tttgttgagt ggaaaaagga atctgtgctg cgtatgcatg
     8521 caaataagta gagaattttt cttctggtcc atgtagttca atgctgtaat tgaaccgatg
     8581 ctcacctaga cactgtgctt ctggtgttgg aaaatatccc caatcgccta gttctcccac
     8641 actgcgaagt agcgtcactg cgatttgtcc gtcttctgtt acttcatatt cattcaaacc
     8701 aaagtttccg actgttacac cgtactcttc atcatgaata ttgacaaatg cttgctgatg
     8761 ttgcggattc gtggggtttt cccaactttt agacacatgg tttgggcgct tcaccacttc
     8821 aaaaatacta tctgcttcgt gatgttccac atgtaacttt gtaggaaaca agacacgtaa
     8881 gcgatgatct ttcatctgat tgtcgagagt cgtttcaaag tcgatttttt tactatcttt
     8941 tctgattgtc atgattgttt ttatttgaag gattcgtttt tctttggatc gttctgcttt
     9001 tcgataacga aattccacaa ccatctgctg ttctttttct aaacgctcgt ctgcagaaac
     9061 agggatttcc atttcctgta gaatacttat ttttgctatt tccggtgtat ctactaatac
     9121 ttttacttca gagttttctt tgttactgga taggattggc tgatcacaga aaggttgttt
     9181 aaagatatac tcatttccta tatcgccgat atcttcaaaa atatgcagat tattcaactt
     9241 tcgtttgttt ttgcgatctt ccattgtaat ggtaccattt ttttcaatcg tcaaatgtag
     9301 agggccgttt tcgatgattc gtccactctg atgaatcatt gtttcctttt cttctgcttt
     9361 cgcttcccct tctacaagcg cgaatgtttc ccaagaaaat gctggcatgt tttctaatgg
     9421 taaagtcact ttgatcattc ttgccatata tggcacacgg aaacggtctt tcggcaaatc
     9481 ataaccaaat tggacagttt cttcagataa aatagctggt atagtggccc cactttttgt
     9541 ttctacatga tacttacgtt taggcaaatt ttctaactct tgatatagtt gttcaggtat
     9601 tccttcagcg aatctttttc tttctaattc gatggttatt tctgcttcac cagttttatc
     9661 cattcctgca gtatttacaa tgacaaatgg aaagctgtct tttgggaatc cggtcgtatc
     9721 gattgctgcg gtcagctgtt ctaaggaatc ttgtgcaaga tactttccaa cttcatctgc
     9781 tttttcaaaa cgaggtatca tttcccgatg gacagaatcg actgaacaac cgcaaatact
     9841 gtcgtgagga tgattttgca ttaatgtctt ccaagcataa tctaattgat catggggata
     9901 gtttccacta acttcataag ccatcgtcgc aagtggttct gtaatatttt ctagctgtcg
     9961 ctggaccttg gtattccatt gttttaagta aacccgagca gatgctgtat tggctaaagt
    10021 ataccagcca tccgtttctt gacttgtcaa ttctccttct actgagccta agtcttctgg
    10081 cacatctttt tgaactgctt ccagatagtc agtaaaatta gaatgaataa actcgtaatc
    10141 tggaaacagt tcatttgcga gatggattgc ttttgatata tctttttgta ccggttgatg
    10201 atcaacacca ttcatcatca ataagtggtt cgtagaggca tattgttctg catccgctaa
    10261 tttttgcttc caaaaggcaa gagctgcttc tttttctact ggaatctcat tcccgttgct
    10321 ataccaattg gcaaatagca gaccaaagat tgctgtttga tcagggcctt tccaccacat
    10381 ctctgagtat tgggaagaat agttttctgc ttccaaaact tgattatcaa agccaattgg
    10441 ttttacacca cggccaaacg ctgctgctga aataccagct tgtttcatta attgaggcgt
    10501 ctgtcccata tttccaaatg tatcgggaaa atagcctaac atgacagggg ttccccattt
    10561 acggctttct tccatgccaa tcagcatatt tctcacgttc gattccgagc tgataagaaa
    10621 atcatcttgc aagatataaa atggtccaat tctcaatttt ccttcattga ttgcttgttg
    10681 gattgcctgt cttttttctg gccttacttg aagatagtca tctaaaataa tcgtttgccc
    10741 atccaaatga aaactgttga aggacggatc aacttcaaat agctctagta gatcgtctat
    10801 caattcaatc agtcgcatgt gatgctgttc gtaagccatg taccattcac gatcccagtg
    10861 gctatgagaa atgatatata ctttcttttt catttacgat acctcctact tttctactct
    10921 aatatcaaag taatccatga ctaattcgca aaacatcata tttgcccaag aaaaccattc
    10981 tctcgtataa ttttcaggat tgttcacatc gaatccttcg tgcataaggt gtgttcctgc
    11041 gtcagttgct acaagtaaat tcaatatccg ctctttttca gatttgtctg acgttgtcat
    11101 tccttccata gctaatgcaa tcggccaaac gtatttttct ggcgtatggg aggatccaat
    11161 tccttttgcg tactttcctt catagaaata tggattttct ttactcaaca atgttcttct
    11221 tgtttgcaga taatcttcct cttgttcaga acaatatccc aagtaaggtg cggcgatcag
    11281 attgggcaca ttactgtcat ccatgactgt ggcgtttccc attccatcta cttcatatgc
    11341 ataaatcgtt tgcccttttt gattaaccgt tcgtccgtga ttctgaatcc cctcttggat
    11401 ctctttcttc aatttctgtg tttgctgtac aatttcttta tcatcgagta tcgtttgaaa
    11461 aatttcttcc acataaccta acacgaccac cgcaaacata ttggatggta ccagatatcc
    11521 ataacggcaa gcatcatcgc taggacggaa accagaccat gtcattcctg taaaagctgt
    11581 ttttgcacct cgcccgtcat tgatcagcgt atcttctttt cttgtcgtgt ctcgtacaaa
    11641 tgtataagga gagtgttcat gatcttgctc tgttttgaac acgtccaaga tttttttgat
    11701 accttcaata aaagattgat tgaattgatc agtttttcct gtatttttgt aaagaagata
    11761 ggccaactgt attgggtagc ataaagaatc aatttcatat tttctttccc agatccaatc
    11821 gttcattttt gtatgatctg tttggtggcc agcaccgtta gcttcttcgt tgaaggcatt
    11881 cgcatatgga tcgatattga tataaaaaaa ttgctttttg actaacccgc tgatcatgtc
    11941 agctaattcc tcatcttctt ttgcaatcac taaataagga cgaacttgtg cagtagagtc
    12001 tcttaaccac atcgctggaa tgtctcccgt caataaaaag gtggttccgt cattttgctt
    12061 tttgactgtt gtcaacagag tattggcaaa tgctgctgta aaattctttg cccagtccgc
    12121 atggatatcc ccacattttt gtgtgatatc ttccataaat ttctgcaccg attttggtat
    12181 atctttataa accactaata aaaccttctt tctatttgat atatctttaa agattatact
    12241 tcatacagcc aattatgtct acgctttcaa aaaatatctt tttatttact ttttcatcca
    12301 ttatggtatt atttgaatat gatatatcat taagaggtgt ttatatgact caaccattgt
    12361 accagacaat atttaatgat ttgcaaacgg ctattttaac tggtgaatta cctgtagaca
    12421 gccaactacc aactgaaaaa gaactttcac gtacctataa agtaagcaga atcacttcca
    12481 aacgtgcatt gactgaatta gagcaattag gacttattta tcgcgttcgc gggaaaggca
    12541 gtttcgttaa atccgcttct ccggaacaga ttcttgcttc tcctacaaaa acacgacgga
    12601 ttctgtttct tcttccatat gtcgctgatt tgtctgttgg tgattttagc aagggattga
    12661 accctgtgat gcaagataaa cagattgatg tcatgatgac gacgcttgac tttctacaaa
    12721 ataaaacagc aaaagagcta atcaatgaat ttgacggatt gatttattat gctcttgata
    12781 cagaacaaca tcttgactta ctttttgagc tttcattgaa agaattccct gtcgtcatat
    12841 tagataaaaa aatctatgag ttaccttttc ctaccgttct ttccgataat ttccaaggcg
    12901 gtgcgcttgc tgcctcttat ttgatcgaac agggacattc aaaaatcgct tatttgtttg
    12961 gtacgcagac tcatccacaa tccgttcgac agcgttacct tggttacctt gaagcattgg
    13021 acaaagctgg tttgactttc cacacttcac tggatgacaa aagcgcgaca ttagatgaat
    13081 tgctttctta tatcactaag tatgaagtaa ctgcttttgt ttgtgaaaac gatgttgtcg
    13141 ctatccagac catgaatctc ttgagacagc atggttatca tatccctgaa gatttttcaa
    13201 ttatcggatt tgatgatatc caagcagcag ctcttgttga cccgccactg acaaccatcg
    13261 cacaagattt tgaacagtta ggtaagcttg cgggagatgc tttggtcact tggttagaga
    13321 caaaagatcg accatccgat agtaaatttc cagtttcatt catcaaaaga caatcaacaa
    13381 aggagaaata aaatgaatat cggtcaaata gatactagac atgcaacagc gaatcagcca
    13441 aatttctcta atgggaactg ccagccttat actggcgttc cttttgggat gaattatttt
    13501 gcaccgcaaa ctacggatca aaacggcagc tggtggtttc atcctgatga ccgcgttttt
    13561 caaggctacc gtcttaccca tcaaccaagt ccttggatgg gtgatttcag tcatatgctg
    13621 tttacgccga tcaatggaaa actacaggag aacacccttt tccatgctca aagttcttac
    13681 cgcccagaag aaagtatatt ttgcccgact catctgtcga tctgtcaatt acgttatggc
    13741 atctgttcta cattgatacc tagcatgtat ggcggaatct tatctattga ttacagcaaa
    13801 aatgacagcg gtttactttt atcctttccg ggacgccatc agctctttgt tatagatcct
    13861 tacacagtgg aaggaaaaat cacacatttt gctggttcag aagatcctga tttttctttt
    13921 tattttatat tgagatttga atatccttta acaattgatg agttagctgt ttccaacaaa
    13981 aatagcgact cgatcccgtt gtattttgaa cagacaaaaa aacaaactat ccgatttggg
    14041 acatctttta tcagctcgga acaagcagct tataacctgg aactagaaca aaaccaaagc
    14101 aaagagactt accttgctca cagtcaagaa caatggcagt cctattttga tcgtatacag
    14161 ctcacacacc atgatcaaca acaattgcgg acgttttatc ataatttata tcgggtgttt
    14221 ttatttccac aaacatttta tgaaattgat cctagtgggc aaaaaatcca ctatgatacg
    14281 actagtcgat ctataaaacc tggtgttctc tatacaaata atgggttttg ggatacgtat
    14341 aaaacggttt atcctcttta ttctttgatt gctgtagaaa aatacgaaga aatgctggaa
    14401 ggttttttga acagctatag agaaagtggc tatttgccaa aatggctgtc tcctgatgaa
    14461 cgtggactta tgcctgggac attgatcgat gcggtcattg cagatgctgc tgtaaaagat
    14521 atccgacgag acttgatgcc tgaatttcta acggcaatgg aaaaaggtgc tacggttcaa
    14581 agcaaacatc caaattatgg acgccaagga acagaagatt acttgacata tggctatgtt
    14641 cctaatcact accacgaatc agtcaatcac acattagact atgcttatag cgatttttgt
    14701 attagccaag ttgccaaggt actagaagaa gaggaaatga cgaagaaata tcagcagcaa
    14761 tcattgaact atcaaaatat ctttgactat gagtcaggat ttatgcgttc aaaagataaa
    14821 gatggcaagt tcagagaacc attttcagct aatcgttggg gacaagatta tgcagaagga
    14881 agtgcttggc aaagcagctt tgctgtttat cacgattttt caggactgat cggaaaatat
    14941 ggtggcagtg agagatataa agaaaaactc attgagctgt gcaatcagcc cccaacgttt
    15001 gacccaggag gatatggttt tgagatccac gaaatgagtg agatggccgc gatagaattt
    15061 ggacaattag ctatttcaaa ccagccaagt tttcactatc cttacttgtt cagttatata
    15121 ggacaacctg aaatggcaca gccactgctt aaacagttga tgactcagac cttcgacgat
    15181 tctccaatgg gttaccctgg agatgaggac aatggaagta tggccgcttg gtatatcttc
    15241 aatagtctgg gattttatcc tgtcacgcct ggaaccggtg aatatgttat cggtatgcca
    15301 ttaattgaca aggctgtttt gcttttgtca aatggaaagg aattgacgat tgaagcaacg
    15361 cctaatcggc cgcagcaaca atttatccat aaaatcaaac ggatggacag agaatatacg
    15421 aaattatatt ttacccatca agatttgctg aaaggcggca cgatctctta ccaattaggg
    15481 atcatccctc accctaagaa atatcaagca aaagatttgc ctttttcctt atcaaactat
    15541 aattaacaag aggttgtgaa aaagctgtcc tgcatcaagt aataagaaca gccaagcaaa
    15601 aatagatcct catatttttc gtttggttgg tcttattacc gcagatgcaa gcttttgaac
    15661 accgtttatt gagaggttgt gaaatca
//
LOCUS       DACEEZ010000067        15230 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20313_2017
            SAMN12639966-rid9879223.denovo.067, whole genome shotgun sequence.
ACCESSION   DACEEZ010000067 DACEEZ010000000
VERSION     DACEEZ010000067.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639966
            Sequence Read Archive: SRR10023158
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 15230)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15230)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741683.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741683.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:44:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,016
            CDSs (total)                      :: 2,962
            Genes (coding)                    :: 2,889
            CDSs (with protein)               :: 2,889
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 36 of 73
            Pseudo Genes (incomplete)         :: 29 of 73
            Pseudo Genes (internal stop)      :: 18 of 73
            Pseudo Genes (multiple problems)  :: 8 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15230
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639966-rid9879223.denovo.067"
                     /isolate="E20313_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            278..787
                     /locus_tag="IWO89_002086"
     CDS             278..787
                     /locus_tag="IWO89_002086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HAQ0456812.1"
                     /translation="MVQGIERGVYVVDKIQFNQVIEEFIKVNNLMTHIQKRPIRITND
                     LKLSTSMIHLIDVIGNYPETSITELADRLGVTKGAVSQQIPTLEKMNLISISQKKGNK
                     KTKLVSLTKYGRKVYESHNYLHEELYSSINEALESFSPDQISTIKQILEKVSYTINEY
                     QVSLTRKED"
     gene            880..2042
                     /locus_tag="IWO89_002087"
     CDS             join(880..1156,1156..2042)
                     /locus_tag="IWO89_002087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611768.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HAQ0456813.1"
                     /translation="MSRRQRRTYSKEFKQQVVDLYLAGKPRAEIIRDYELTTSAFDKW
                     VKQAQTTGSFQEKDNLIPEQVELIALRKKNKQLEMENDILKQAALILGPKRQVIDTNK
                     HKYSISAMCKVLNISCQTYYYQAKPVEDESELEELVQEEFIRNRKAYGTRKLKQCLAN
                     LGLQVSQRRIGRMMKQRGLRSTYTLAHFKFHSSTCNEAKTANILDGTFLQEKSLEAIV
                     TDLTYVRVGKKWQYICLILDLFNREIIGYSCGEKKDATLVKEAFAKIPYSLNNVQLFH
                     TDRGKEFDNQTIDEILWAFNITRSLSKKGCPYDNAVAESTYKSVKVEFVHQYRFETLA
                     NLRLELFDYVHWWNTIRLHGTLGYETPISFRQQRLAQRILDNELGCDTGGEAP"
     gene            2322..3302
                     /locus_tag="IWO89_002088"
     CDS             2322..3302
                     /locus_tag="IWO89_002088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016179831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family transcriptional regulator"
                     /protein_id="HAQ0456814.1"
                     /translation="MKKIGIREVAKEANVSPTTVSRVLNDRGYLSEETKRKVFDAMKK
                     LEYYPNELARALFKNETYTVGLIFPTITNPFHAELIQDIEFELSSRGYKVLLCNSLNN
                     PTKEKEYLTMLRKNQVDGIIVGTHNKDIADYDIPGLPIVAIDRSLGENTVTVSCDNYR
                     GGELATQLLVNHGCQNILCIRGDSKIKLPANSRTLAYKDVLNKFMLEPLVLEVPFVKE
                     INEKNEIIKTYLETHPNIDGVFAGDDLLASLAINNLGSSGKRVPKEVKVIGFDGAQQT
                     LIYNPELSTIQQPIDQISKVAVKKLLNMIKGEKETDELSLPVKLIKKNTA"
     gene            3424..4842
                     /locus_tag="IWO89_002089"
     CDS             3424..4842
                     /locus_tag="IWO89_002089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-fructofuranosidase"
                     /protein_id="HAQ0456815.1"
                     /translation="MEKLLIKSNNHQLYYQPKDVWVGDVMPYGKEGKFFIYHQRDTRN
                     PVPFGEPFGWALATTKDFVHFEDFGESLLRGDDNERDQFIYAGSVFEANGKVHAFYTG
                     YNREFLAQGKTSQVLLHAESDDFVHWEKSETALELEPQEGYDIRNWRDPFVVWDDEKE
                     EYLLILGARKGQDKHLQTGRLVEFTSKDLTNWEFKGDFWAPGLYTMFEMPELFKIGDW
                     WYLVYSEYSDGNKILYRMSQSLEGPWIKPKDEAFDGRAYYAGRTAYDGKRRVLFGWVP
                     TKDQENDKNNYLWGGTLVPHEVYQKEDFSLGVKPIDEVWNAFINLHDIADVEIETADT
                     REEVTLVKNSGTLFNLETTISFDENLSDFSLRLYKNLETDESYEYRFNLDEGRLTFDK
                     NPCYPWFQYMDKGLIRPISLKPNQSYKLKVIVDDNIMTLYIDGIALNARVHGCFGQAI
                     TITATNGSMTAINTKFSNEIKK"
     gene            4913..6382
                     /locus_tag="IWO89_002090"
     CDS             4913..6382
                     /locus_tag="IWO89_002090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016179833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAQ0456816.1"
                     /translation="MDYKKIAEDVLENVGGKENISSATHCITRLRLILKDKSKANMEM
                     LEEIEGAKGVLFNSGQLQVIFGTGIIEKVYKEFVELTGAKEVSVSEMKDEGVNNSGNR
                     LQRAFKVFSDIFIPIIPAFIGAAMVLGIRSLITTPGMFGMDGSFADHNVFLNDLSKFL
                     GIIATTFDYLPVLVMYSATKRFGGNPILGLLVGFVMIHPGLANRNDFVMGTIKPDYWH
                     LFGLSIPAVAFQGGVFPAILTSWFLAKVEKITNKYSPQVLAYILVPTVTIVLANFALF
                     LVFGPLGNFIGNVLGGIIDFLYMKMGAFGAFVFAGALQPLVVTGTQHAIQGIEANLVA
                     TTGFNYIQPIWSVSIIAQGGGCIGMYLLFKKKSKDRDIAISSFIPTLVGITEPAIFAV
                     NLKYSIVPFVCSFIGAGFGGAFMKIFDVKAIGQGLTVLPGLTIANPVLPYIFGNLIAF
                     ILPIIFILAYDKYKVFIPEAKHRKFAEKTDSKKLPVQVK"
     gene            6392..6859
                     /locus_tag="IWO89_002091"
     CDS             6392..6859
                     /locus_tag="IWO89_002091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HAQ0456817.1"
                     /translation="MLNFFRKNEISVVAPATGVFEKLEKLSDPVFSKGMMGQGFAIDA
                     KNETIVSPIDGFVSSIFPTKHALGIKTKSGIEILVHVGIDTVELNGEGFDIKVQEGQK
                     VKAGDILMTINFVVFEKNHKTKDVIVVFPDYQDEIFIPKLEEVKQGQELFVLG"
     gene            6895..7767
                     /locus_tag="IWO89_002092"
     CDS             6895..7767
                     /locus_tag="IWO89_002092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HAQ0456818.1"
                     /translation="MVFGAIEAGGTKFVCAIGDKDLNIFEKVSFPTTTPKETMNLVLD
                     FFNHHKDDLEGIGVGSFGPIDIHCESKTYGFITSTPKLAWQDFDFIGSLKKQLNIPTA
                     WTTDVNASCYGEYVSGNGRGLSSVVYYTVGTGIGGGAIQNGNFIEGFSHPEMGHMLVK
                     RHNKDNFSGTCPFHGDCLEGLAAGPAIERRSGKKGQEVTENDVTWEIEAYYIAQCAYN
                     TTLMLSPDVIIFGGGVMKQKHLKKKVQDKFIELINGYVAIPDVDNYIMTPKLLDNSGI
                     VGCLALARDVKEKS"
     gene            7848..10382
                     /locus_tag="IWO89_002093"
     CDS             7848..10382
                     /locus_tag="IWO89_002093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAQ0456819.1"
                     /translation="MKKKMKDEVLEYIKNNLKYYDFSAQDIAMKFCIKRNVASHYLNQ
                     LFSDGKLLKNDSVRPVMFKYNQQKPKDCFSKFIGADISLKSTIDKCKATVMYPPNGLP
                     LIIKGNSGVGKSFLASLIYQYALDRKVIHNDAKFVVVNCADYANNPELLSAVLFGYKK
                     GAFTGAEKDTAGMLSSANGGYLFLDEVHNLSAENQEKLFLLMDSQKYRKLGESVQWEY
                     ANVRLILATTEDKNTSLLATFRRRIPAEITLPDYNNRSTSEKIQLLFEFLKDEAITID
                     SKIFCSIELFTDLLSKTFEGNVGELRNEIKLLCAEGYLGNKRKSSIYLGDKLDSGFWI
                     DPEISIDLKKMFLKSLSEIDLIELFQNHISIEESMSQLRDRILKECAGSSEYNYLESD
                     EFIALRNYVVNKISPIIDSTGLCLLDEFLADISLFIMFIDVIEETSRNFLLSKFRKFR
                     MKNDKQKLLANEIWSSLEVEESKQELLLKWILFLVKKFYVKIPETHCLIVMHGKITAS
                     AIARETNKLLNTYVYEAFDMPIEGETSDLINLINNFCKSIDTTNGLILLVDMGSLEQM
                     YEKIESNVIGDLVILNNVSTALAIECGIQVCQKKPISHFYQMDFDSFQVKVQYYKGLS
                     QKKNVIVSCLSGEGISEKVKDILKRYLDNQVEILIFDFNALKKIAKEKDTMIFKNTIC
                     VLSTTEFYIQGIDCLNLENLINGNQTLEKLNKYMDSDSCEFCLNELVKLFTIEGASTK
                     LRFLDSKKVFNEIEKVLYLYEKYYDVKIPSFLRINLFLHLSGMIERIMIGDGITNEHL
                     KEGAQLFDTFLNVSKDFFSEIKDIYCIEIPKGEYELIYTIFEQTIF"
     gene            10538..11311
                     /locus_tag="IWO89_002094"
     CDS             10538..11311
                     /locus_tag="IWO89_002094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAQ0456820.1"
                     /translation="MAFWQTFLVVLYGFFINWDKEGPKIGTSQPVVAGFLTGLIMGDI
                     QTGLYIGGTLQLMTLGITSFGGASVPDYQTAALIGTYLAISTNQDASIGITLAIPVAM
                     LIVQLDVLKWSTNIFFQQKAEKFAQKEDYQKLEWMQYLASINTSFVSGIPVLLTVILG
                     PTLVGSAINYIPEWVTGGLKVAGGLLPAVGIGMLLKYLPTKDYFAYLLVGFVLAVYFQ
                     IPILGISLLGAAIALIVYKNRSNNTQVAPAATGGFDEDE"
     gene            11304..12095
                     /locus_tag="IWO89_002095"
     CDS             11304..12095
                     /locus_tag="IWO89_002095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HAQ0456821.1"
                     /translation="MNKKLTKIDKQVIFRWILNGATSLNYEKMEGLGYAYSMLPFIKD
                     TYKDDPEGMKQSVLAHLQFFNTTPYTAPYIIGMNVGIEKEEKNKSLEAVSALKTGLMG
                     PLAGIGDSVFVVIPWTIFGAIAANMALEGSFVGIILWILASILLRITAYPLYLAGKES
                     GASLLTQIETKLKSITDAVSILGLMVVGALIATVVKVNVGFEFKQGDLTMTGNEIFDQ
                     VMPGLLPACVVAIVYYLLGRKVKPVYVILLILVVSIALRALNILK"
     gene            12105..12596
                     /locus_tag="IWO89_002096"
     CDS             12105..12596
                     /locus_tag="IWO89_002096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAQ0456822.1"
                     /translation="MDEKMKGIIHVRIDDRMIHGQVATQWTNQLSANRIMVINNEVAN
                     DDVKKAVVRLAAPPNVRTSIITREVAVKNILSGKYEGQKVLIVAVSPIDVKFLIDNGL
                     PITSVNVGNLSRRNGTERIRPTINITEEEREAFKELISDGVEVTVIQTPRDSKEFLKD
                     FIY"
     gene            12614..13024
                     /locus_tag="IWO89_002097"
     CDS             12614..13024
                     /locus_tag="IWO89_002097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HAQ0456823.1"
                     /translation="MEFIITSHGNIAIGFVDTLKMFFGDNLNCHVVTLGDAGIEEFRE
                     NIEKIVSQVKNQEVMVFADLIGGTPFNEFAKRSEVFQNGFQLLGGMNLGILIEAVNLR
                     LQGKKSDDIIENLRNMNTIACLADMQRHTNEEDE"
     gene            13028..14428
                     /locus_tag="IWO89_002098"
     CDS             13028..14428
                     /locus_tag="IWO89_002098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-fructofuranosidase"
                     /protein_id="HAQ0456824.1"
                     /translation="MQKIFYKPKDFWFGDCMPFYKDGTFYLFHQRDTRNPKPLTDPFG
                     WSLVTTTDFVKFEEYGEVIHRGGNDDIDQYIYAGSIVQGINEYLALYTGHNRKAKLDG
                     KTSEVLMIASSKDLIHWKKDGEAAKLVPQEGYDKNDWRDPFAIFDQDHGKYVLILGAR
                     KPASKKKPTGRLVYFESEDLKDWNFKGDYWANNEFNMIEMPDIFEIDGTWYLLFSEYC
                     EDKKTKYRVGAGLFSDWKSPVDEAFDGKAYYAARTVGTNETGRFLTGWVATKEGENDQ
                     NNWDWGGALVPHQIIQKTDKTLGVKLPEKIIENFKEITPFSSFKLEKEFGKKERVIVP
                     KIEDYFLFETTLTITSLTKEFALKLFVDSDTQEGYEFRISLINNQLTFGKTPNYPWPQ
                     MFDKGLQRPLFLEIGKEYELKLIVDDTIAVLYIDNVALSTRMYDKVGTALSMTVTEGA
                     IKVDKLDYSINYECGF"
     gene            14451..14825
                     /locus_tag="IWO89_002099"
     CDS             14451..14825
                     /locus_tag="IWO89_002099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016179839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactoylglutathione lyase"
                     /protein_id="HAQ0456825.1"
                     /translation="MKLNGLQHIGIPTKDFGASKIFYESLGFSLINEEMNVGSKVGFF
                     ELNGTIIEIWEDKTTECRGAIDHLALDTDDIDEVFNSIQDLGYELLDKNIMELPFWEN
                     GIRYFNFYGPNHEIIEISQMKK"
BASE COUNT         5286 a         2135 c         3046 g         4763 t
ORIGIN      
        1 ttttttattt gtctcgctta attatacaaa cggcgtgata aaaaacgaga aatgaattat
       61 aggtttagac ctttatcatt tctcgtttta ttttattgta tcaaggctct atcataagtg
      121 gtaaaatcta cttttaaatc acatgtaatc attgataagt ccagtgtttt agcggataat
      181 cagcttttta tttatttttt cttataaatt tgataaatag aaattagtat agccctaaac
      241 aaattatttg tgttttattg tttagggcta tactatattg gtacaaggaa tagaaagagg
      301 tgtttatgtg gttgataaaa ttcagtttaa tcaagttata gaagaattca tcaaagtaaa
      361 taatttaatg acccatattc aaaaacgacc aatcagaata actaatgatc taaaactcag
      421 tacgagtatg attcatttga ttgatgtgat tggaaattat cctgaaacta gtattacgga
      481 attagctgat cgattaggcg ttaccaaagg agctgtatcc caacagattc ccactttaga
      541 gaaaatgaat cttataagca tttctcaaaa aaaaggaaat aagaaaacta aacttgtatc
      601 gttaacaaaa tatggtcgaa aggtgtacga atcacataat tatttacatg aagagcttta
      661 tagctcaata aatgaagcgt tagaatcatt ctcaccagac caaatttcaa ccattaaaca
      721 gattctagaa aaagtatctt atacaatcaa tgaatatcaa gtaagtctga caagaaagga
      781 agattgacat gaaaaaaatg atttttgaat cggctacact agactagaca gaaaaaataa
      841 ggtgtgtaaa ataaatgaaa acacactgga ggaattttta tgtcaagacg tcaacgtaga
      901 acctattcaa aagaattcaa acaacaagtc gtcgatcttt atctcgcagg aaagccccga
      961 gctgaaatca ttcgagatta tgagctgact acctcggcct ttgataagtg ggtcaagcaa
     1021 gcgcaaacta ctggctcgtt tcaggaaaaa gataacttga tccctgaaca agtagaacta
     1081 attgcattac gaaagaaaaa taaacagctt gaaatggaga atgacatttt aaagcaagcg
     1141 gcgctgatat tgggacgaaa agacaagtaa ttgataccaa taagcacaaa tactcaatat
     1201 cagcgatgtg taaagtgtta aacatctctt gccaaaccta ttattatcaa gccaaaccag
     1261 ttgaagatga atcagaactg gaagaactcg ttcaagaaga atttattcgc aatcgaaaag
     1321 cttatggtac caggaagctg aagcagtgct tggccaatct aggtcttcaa gtgagtcaac
     1381 gcagaatcgg ccgtatgatg aaacagcgtg gcttgcgttc aacatacaca ttggctcatt
     1441 tcaaattcca ttcttctact tgtaacgaag ccaagaccgc caatattcta gacggaacct
     1501 tcctacagga aaagtcatta gaagcgattg taaccgacct tacctatgtt cgtgttggga
     1561 agaaatggca gtatatctgc ctgattcttg atttgtttaa tcgagaaatc atcggctatt
     1621 catgcgggga gaagaaagat gcgacgttag tcaaggaagc ttttgcaaag attccgtact
     1681 cgttgaataa tgtacaactt tttcacacgg atcgtggaaa agaattcgat aaccagacga
     1741 ttgacgaaat tttatgggcc tttaatatca cgcgttcctt aagcaaaaaa ggctgcccct
     1801 atgataacgc cgttgcagaa tccacctata aatctgtcaa agtagaattc gttcatcaat
     1861 accgctttga gacattggct aatttgcgat tagagctatt tgattacgtc cattggtgga
     1921 ataccatccg gctgcacggc acgttaggct atgagacacc gatcagtttt cgacagcaga
     1981 gattggcgca gcgaatcctt gataatgagc tcggatgtga taccggtggc gaggcacctt
     2041 aattatttga atgagcttct gccttagaga atcagattcg aggaggctcg ttgtcaaggg
     2101 cagtcggaac accagcagca agcttacgca ataccttata atttttgtca aaaaaagtgt
     2161 tgccattcca catatatctt taaacagcta ttgccagata aaaataaatg atattatata
     2221 tataaatact tatccgaaaa ggggaatgag agagtcaagg ttcttggaat gtcgaatagt
     2281 cgagtaggta gattttctat ctacaacctt gttgtttaaa aatgaaaaaa attggaatta
     2341 gagaagttgc gaaagaggca aatgtttctc ctacgactgt gtcgagggta ttgaatgatc
     2401 gtggatattt gagtgaggaa acaaaaagaa aagtttttga tgccatgaaa aaattagaat
     2461 actatcctaa tgaactggcg agagcacttt ttaaaaacga aacatatacc gtaggtttga
     2521 tttttcctac aattactaat ccatttcacg ctgagttaat tcaagatatc gagttcgagc
     2581 tttccagcag gggatataaa gtgttgctgt gcaatagctt aaacaatcca acaaaagaaa
     2641 aagagtactt gacgatgttg agaaagaatc aggttgatgg aattatagta ggaacgcaca
     2701 ataaagatat tgcggattat gatattccag ggctacctat tgtagcgatt gatagaagtc
     2761 ttggtgaaaa tacagttaca gtatcttgtg ataattacag gggtggggaa ttagctactc
     2821 aactacttgt aaatcatggc tgtcagaata tactgtgtat tcggggagat agtaagataa
     2881 agctacccgc gaatagtagg actttagcat ataaagatgt tttgaataaa ttcatgctgg
     2941 aaccgttagt cctagaagta ccatttgtaa aagaaatcaa tgaaaaaaat gaaataatta
     3001 agacatatct ggaaactcat ccaaatattg acggggtttt tgcaggagat gatttattgg
     3061 cttctttggc gattaacaac ttaggaagtt cgggcaaaag agttccaaaa gaggttaaag
     3121 tgattggatt tgatggagct caacaaacat tgatttataa tccagaatta tcaactattc
     3181 aacagcctat agatcaaata tctaaagtag cagtaaaaaa attattgaat atgattaagg
     3241 gcgaaaagga gacggatgaa ctttcacttc cagtaaagct cataaaaaaa aatacagcat
     3301 aagttaaatc ggcgaaaaat tccttcgtcg atttaaaaat atttttatga aacgggttga
     3361 catatgaaac gggttgacat atattagttg tgaagataaa aaccttttca ctggaggaag
     3421 aaaatggaga aattattaat taaaagcaat aatcatcaac tttactacca acctaaggat
     3481 gtttgggtcg gagatgttat gccttacggg aaagaaggaa aattctttat ttatcatcaa
     3541 cgtgatacta gaaatccagt tccttttgga gaaccgtttg gctgggcttt agcaactacg
     3601 aaagattttg ttcattttga agactttggg gaatctcttt taagagggga tgacaatgag
     3661 agagaccagt ttatctacgc tggttctgta tttgaggcaa atggaaaggt tcacgcattt
     3721 tatacagggt ataatcgtga attcttagcg cagggaaaga catctcaagt gttgcttcat
     3781 gctgagagtg atgattttgt tcattgggaa aaatcagaaa cagcattgga gttagaacca
     3841 caagaagggt atgatattcg aaactggaga gatccatttg tcgtttggga tgatgaaaaa
     3901 gaagaatatt tgctaatttt aggggcgcga aagggtcagg ataaacatct acaaaccgga
     3961 cgtttagtgg aatttacatc aaaagattta actaattggg aattcaaagg tgatttttgg
     4021 gctccgggat tgtataccat gtttgaaatg ccagaacttt tcaagattgg tgactggtgg
     4081 tatcttgtct attcagaata ctcagatgga aataaaatac tctatcgaat gagtcaaagt
     4141 ttggaaggtc cttggattaa gcctaaagat gaagcttttg atggacgggc ttattatgca
     4201 ggtagaacag cttatgatgg aaaacgtcga gtcctatttg gatgggtacc aacaaaagat
     4261 caagaaaatg ataaaaacaa ttatttatgg ggaggtactt tggttcctca tgaagtctat
     4321 caaaaagagg atttttcact tggggtaaaa cccatagacg aagtgtggaa tgcattcata
     4381 aatttacatg atattgccga tgtagagata gaaacagcag acacaagaga agaagtaacg
     4441 cttgtcaaaa atagtggaac tttgtttaat ttagaaacaa caatttcatt tgatgagaat
     4501 cttagtgact tttcactgag attatataaa aaccttgaga cagatgaatc ttacgaatat
     4561 cgcttcaatt tagatgaggg gcgtcttaca tttgataaaa acccttgtta cccttggttt
     4621 caatatatgg ataaaggact tattagacct atatctttaa agcctaacca atcttataaa
     4681 ctaaaagtga ttgttgatga caatataatg acactttata ttgatggaat cgctcttaac
     4741 gctcgtgtac atggttgttt tggtcaagca attactatta cagcaacaaa tggatcaatg
     4801 actgcaataa ataccaagtt ttcaaatgaa ataaagaaat aataaacgtc aatcatgtgg
     4861 caggtctgaa aatcaaacat gacctgtcac atttaaaagg gaggataaat gaatggacta
     4921 caaaaaaatt gccgaagatg tattggaaaa cgttggagga aaagagaata tctcaagcgc
     4981 tacacactgc attactcgtt tacgtttaat tcttaaagac aaaagcaaag cgaatatgga
     5041 aatgcttgag gaaatagaag gtgcaaaagg tgttcttttc aatagtggac agttacaagt
     5101 aattttcgga acaggtataa ttgaaaaggt ttacaaagag ttcgttgaat tgacaggagc
     5161 taaggaagta tccgtttctg aaatgaaaga cgaaggagtc aataattcag gaaatagact
     5221 tcaacgagct tttaaagttt tctcggatat ctttattccg atcattccag cttttattgg
     5281 ggcagcaatg gttttaggaa ttcgttcgtt gattaccact cctggaatgt ttggaatgga
     5341 tggctcattc gctgatcaca atgtattcct aaatgattta tccaaatttc tgggtattat
     5401 tgcaaccaca tttgattatt taccagtttt agttatgtat tctgcaacaa aacgttttgg
     5461 cggaaaccct attttaggat tacttgtagg atttgttatg attcatccag gactagccaa
     5521 tcggaatgat ttcgtcatgg gaactataaa gcctgattat tggcatttat ttggattaag
     5581 cattccagca gttgcgtttc aagggggtgt ttttccagca attttgactt cttggttttt
     5641 agcaaaagtt gagaaaatta caaataaata ttctcctcaa gtacttgctt acattttagt
     5701 cccaactgtg acaattgttc ttgccaactt tgcactattt ttagtgtttg gtccattggg
     5761 gaattttatc ggtaatgttt taggtggaat cattgatttc ctatatatga agatgggtgc
     5821 ctttggtgct tttgtttttg caggcgcgtt acaaccatta gttgtaacag gaacacaaca
     5881 tgctattcaa ggtattgagg caaacttagt cgcaacaact ggattcaatt atatccaacc
     5941 tatttggtca gtttcaatta ttgcccaagg tggcggttgt attggtatgt atttactctt
     6001 taagaaaaaa tctaaagata gagatattgc gatttcaagc tttattccaa cactggtagg
     6061 tattactgaa ccggctatct ttgctgttaa tttgaaatat tcaatcgttc cttttgtttg
     6121 ctcgtttatt ggtgctggtt ttggtggagc atttatgaaa atttttgatg taaaagccat
     6181 tggacaagga ttgactgtat tgccaggtct gactattgct aatccagttc ttccttatat
     6241 atttggtaat ctcatagcat tcattttacc aatcattttt attctcgctt atgataaata
     6301 taaagtgttt atccctgaag ctaaacaccg taaatttgca gaaaaaactg actcaaaaaa
     6361 attgccagtt caagtaaagt gagatgagag catgttaaat ttttttagaa aaaatgaaat
     6421 aagcgttgta gcacctgcaa ccggtgtttt tgaaaaactg gagaaattaa gtgatccagt
     6481 cttttcaaaa ggaatgatgg gacaaggatt tgcaattgat gcaaaaaacg aaacgattgt
     6541 atctccaatc gatggttttg tttcaagtat ttttcctaca aaacatgcct taggaataaa
     6601 aacaaaatca ggaattgaga tacttgttca tgtaggaata gatacagttg agctaaatgg
     6661 tgagggattt gatattaaag ttcaagaagg acaaaaagtt aaggctgggg atattcttat
     6721 gaccattaat tttgtagttt ttgaaaagaa tcacaagact aaagatgtca ttgttgtttt
     6781 tccagactac caagacgaaa ttttcattcc gaaacttgaa gaagtaaaac aaggacaaga
     6841 attgtttgtt cttggctaat gataagtaat taataataga atcgaggaaa atgtatggtt
     6901 tttggggcta ttgaagctgg tggaacaaaa tttgtttgtg ccattggaga taaagatttg
     6961 aacatatttg aaaaagtcag ctttcccacg actacaccca aagaaacaat gaatttagtt
     7021 ttagattttt ttaatcatca taaagatgat ttagaaggta tcggagtggg atcgttcggt
     7081 cctattgata ttcattgtga atctaaaacg tatggcttca ttacatcaac gccaaaactt
     7141 gcttggcagg attttgattt tattggaagt ttaaaaaagc aacttaatat tccaactgct
     7201 tggacgactg atgtaaatgc ctcttgctat ggtgaatatg tttctggaaa tggcagaggc
     7261 ctttcaagtg ttgtttacta taccgtagga acgggcatag gaggtggcgc tatccaaaac
     7321 ggaaacttca ttgaaggttt tagtcatcca gaaatgggac atatgcttgt aaaacgacac
     7381 aataaagaca acttttctgg gacatgtcca ttccacggag actgtttaga gggcttagct
     7441 gctggtccgg caatcgaaag aagatcaggg aaaaagggac aagaagtgac tgaaaatgac
     7501 gttacttggg aaatagaagc ttattacatt gctcagtgtg catataatac aacattgatg
     7561 ttatcacctg atgtcattat atttggtggc ggagtaatga aacaaaaaca cttaaaaaag
     7621 aaagtacaag ataagtttat tgagttgatc aacggatatg tcgctattcc cgatgttgac
     7681 aattatatta tgacaccaaa attgttagat aattctggta ttgtaggttg tttagcttta
     7741 gcaagagacg ttaaagaaaa atcttaattt ggaaaaaaga tcgtctcgac tatttgtttg
     7801 gttgatgcga tctttttttc aattagtaat tgaagggacg cctaaaagtg aaaaaaaaaa
     7861 tgaaagatga agtattagag tatatcaaaa ataatttaaa gtattacgat ttttctgctc
     7921 aagatattgc aatgaaattt tgtattaaaa gaaatgtcgc ttcccactat ttgaatcagt
     7981 tattttcaga tggaaaatta ctaaagaatg attctgttag accagtaatg ttcaagtata
     8041 atcaacaaaa gccaaaagac tgcttttcta agtttattgg agcagacata tctttgaaat
     8101 ccactataga taaatgcaaa gctactgtaa tgtatccccc taatggcctc ccattaataa
     8161 taaaaggaaa cagtggcgtt ggaaaaagtt ttttagcaag tttgatctat cagtatgctc
     8221 ttgatagaaa agtaattcat aatgacgcaa aatttgttgt tgtgaattgt gcagattatg
     8281 caaataatcc agagctattg tcagcagttt tatttggata taaaaaagga gcctttactg
     8341 gggctgaaaa agatactgca gggatgctat cttctgctaa tggaggatac ctttttttag
     8401 atgaggttca caatctaagt gcagaaaacc aagagaaatt gtttcttctt atggattctc
     8461 aaaagtatcg taaacttggt gaaagtgtgc aatgggagta tgcaaatgtt cgccttatct
     8521 tagccacaac agaagataaa aatacctctt tgttagcaac ttttagaaga cgcatcccag
     8581 ctgaaatcac tctgccagat tacaataata gaagtacatc tgaaaaaatt caattattat
     8641 ttgaatttct taaagatgaa gcaattacga ttgattcaaa aatattttgt tcgattgaac
     8701 tttttactga tcttttatcg aaaacatttg aaggaaatgt tggagaattg aggaatgaaa
     8761 tcaagcttct ttgtgcagaa ggttatttag gaaataagag aaagtcttca atttatttag
     8821 gggacaaact agacagcggt ttttggatag atccagaaat tagtatagat ttaaaaaaaa
     8881 tgtttcttaa atcactttct gaaattgatt tgattgaact ttttcaaaat cacatttcaa
     8941 ttgaagaaag catgagtcaa ttaagagata gaatacttaa agagtgtgca ggttcttctg
     9001 agtataatta tttagaaagt gacgaattta tagcattaag aaactatgtt gttaataaaa
     9061 tatcaccaat tattgattcg acaggtctat gtttattaga tgaattcttg gcggatatca
     9121 gtctatttat aatgtttatt gatgtaattg aagaaacatc aagaaatttt ttgctgtcta
     9181 agttcagaaa atttcgtatg aaaaatgata aacaaaaatt attggcaaat gagatttgga
     9241 gtagtttaga agtcgaagaa agtaaacaag aacttttgtt gaaatggata ctttttcttg
     9301 taaagaagtt ctatgtgaaa ataccagaaa cccattgtct gatagttatg cacggtaaaa
     9361 taacagcttc tgcgatcgca agggaaacaa ataagttgct gaatacatat gtttatgagg
     9421 ctttcgatat gccaattgaa ggtgagacta gtgacttaat taacctaatc aacaattttt
     9481 gtaaaagtat agatacgacg aatggactca ttctgctagt tgatatggga tcattggaac
     9541 aaatgtatga aaaaattgaa tccaatgtaa taggtgattt ggtaatttta aacaatgttt
     9601 ctacagcttt agcgattgaa tgtgggattc aagtttgcca aaaaaagcca attagtcatt
     9661 tttaccagat ggattttgat tcgtttcaag ttaaagttca gtattataaa ggattatcac
     9721 aaaagaaaaa tgtaatagta tcttgtctct ctggtgaggg gatttctgaa aaagttaagg
     9781 atattttaaa acggtatctc gataatcaag tggagatttt gatttttgat tttaacgcat
     9841 taaagaaaat agcaaaggaa aaagatacta tgatttttaa gaatacaatt tgtgttcttt
     9901 caaccactga attttacatt caaggaatcg attgtttaaa tttagaaaat ctaattaatg
     9961 ggaatcaaac cttagaaaaa ttaaataagt atatggactc ggatagctgt gagttctgct
    10021 taaatgaatt agttaaactc tttactattg aaggcgctag tactaagctt cgctttttag
    10081 attccaaaaa agtattcaat gagatagaaa aagtactata tttgtatgaa aaatattacg
    10141 atgtcaaaat cccaagtttt ctgagaatta atctatttct acatctttct ggaatgattg
    10201 aacgaattat gattggtgat ggaataacaa atgaacattt gaaagaggga gcgcaactat
    10261 ttgatacttt tttaaatgta tcaaaagatt ttttttcaga aatcaaagat atttactgta
    10321 ttgaaatacc caaaggagag tatgagttaa tctatacaat ttttgagcag actattttct
    10381 aaatagcata ctagcgtcta aaaaaatgaa agggtgtcca aagtatattt agacgctcat
    10441 ttaattaata tttactgtat tttcccgctt tattggataa tgttagttgg cacagtaatt
    10501 gcttatataa aagtataaga agaaaggaga gaacaaaatg gcgttttggc aaacattttt
    10561 agttgtgttg tatggatttt tcattaactg ggataaggaa gggcccaaaa tcggaacctc
    10621 ccaacctgtc gttgcaggtt tcttgaccgg acttattatg ggagatattc aaactggact
    10681 ttatatcggt gggactttgc aattaatgac actaggtatt actagttttg gtggtgcatc
    10741 agtaccggat tatcaaacag cagcattgat cggtacttat ttagcaatct caactaacca
    10801 ggatgcttct ataggaataa ctttagcaat accggtagca atgttaattg tacaactaga
    10861 tgttctaaag tggtcaacaa acattttttt tcaacaaaag gcagaaaaat tcgcacaaaa
    10921 agaagattat caaaaattag agtggatgca atatttggca tcaattaata caagctttgt
    10981 atctggaatt cctgttttgt taactgtgat attaggccca acgttagtgg gaagtgcaat
    11041 caattatatt cctgaatggg ttaccggtgg tttaaaggta gcagggggac tattacctgc
    11101 tgttgggata ggaatgctac ttaaatattt acctacaaaa gattactttg cctatttgtt
    11161 agttggtttc gttttggcag tttatttcca aataccaatt ttagggattt cgttacttgg
    11221 cgctgcaatc gcactaatcg tatataaaaa tagatcaaat aatactcaag tagcaccagc
    11281 tgcaacagga ggttttgacg aagatgaata agaaactgac aaaaatcgac aaacaagtaa
    11341 ttttccggtg gattctcaat ggtgcaactt cattaaatta tgaaaaaatg gaagggttgg
    11401 gttatgcgta ttctatgctt ccttttatca aggacacata taaagatgat cctgaaggaa
    11461 tgaaacaatc cgttttagct cacttacaat tttttaacac tacgccgtat acagcgcctt
    11521 acattattgg tatgaatgtt ggaattgaaa aagaagaaaa aaataagtct ttggaagcag
    11581 taagtgctct gaaaacaggt ctaatgggtc ctttagcggg aattggagat agtgtatttg
    11641 tagttattcc atggactatt tttggagcaa ttgcagctaa tatggcattg gaaggtagtt
    11701 ttgttggaat tattttgtgg atactggcat caattttatt aaggattact gcttatcctt
    11761 tataccttgc tgggaaagaa tcgggtgcga gcctactaac tcaaattgaa acaaaattaa
    11821 agtctattac tgatgcagta tcaattttag ggttaatggt ggtcggtgca ctcattgcca
    11881 cagttgtaaa agtgaatgtt ggctttgaat ttaaacaagg cgacttaaca atgaccggaa
    11941 atgaaatctt tgatcaagtt atgcctggtt tattacctgc atgtgtagta gcaattgtgt
    12001 attatttgtt aggacgaaag gttaaaccag tttatgtaat tctattaatc ttagtagttt
    12061 ctattgcgtt aagagcgtta aatattttga agtaggtgtt gaatatggat gaaaaaatga
    12121 aaggtattat acatgttcgt attgatgatc gaatgattca tggacaagta gcaacacaat
    12181 ggacaaatca attgagtgct aacagaatta tggttattaa taatgaagta gccaatgatg
    12241 acgtaaaaaa agctgtagtt cgcctcgctg caccacctaa tgttagaact tcaataatta
    12301 caagggaagt agcagttaaa aatattcttt ctggtaaata tgaaggacaa aaagttctta
    12361 ttgttgcagt ttctccaatc gatgttaaat ttttgattga taacggttta ccaataacaa
    12421 gtgtaaacgt aggaaattta tcaagaagga atggaacaga acgcatccgt ccgacgataa
    12481 atattactga agaagagaga gaggctttta aagaactaat tagtgatgga gttgaagtga
    12541 cagtgataca aactcctaga gattcaaaag aatttttaaa ggattttatt tattaagaac
    12601 agaggatgag tggatggagt ttataattac atctcatgga aatattgcca ttggatttgt
    12661 agatacatta aaaatgtttt ttggcgataa tttaaattgt catgtggtaa cacttggaga
    12721 tgcaggcatt gaagaattcc gtgaaaacat tgaaaaaata gtttctcaag taaaaaatca
    12781 agaagttatg gtatttgctg acttgattgg tggaacacct ttcaacgaat ttgctaaaag
    12841 aagtgaagta tttcaaaatg ggtttcaact tcttggtggc atgaatttag gaattttgat
    12901 tgaggctgtc aatttgagat tacaaggaaa aaaaagcgat gatatcatcg aaaatctaag
    12961 aaatatgaat acaattgcgt gtcttgcaga tatgcaaaga catactaacg aagaggatga
    13021 gtgataaatg caaaaaattt tttacaagcc taaagatttt tggtttgggg attgcatgcc
    13081 tttttataaa gatggaactt tttatctatt tcatcaacga gatacgagaa atcctaaacc
    13141 tttaactgat ccttttgggt ggtcactagt tacaaccact gattttgtga aatttgaaga
    13201 atacggggaa gtaattcatc gtggtggcaa cgatgacatt gatcaataca tttacgctgg
    13261 aagtattgtc caaggaatca atgagtattt agctttgtat actggacata atcgcaaagc
    13321 taagttggat ggaaaaactt cagaagtgtt aatgattgca agttctaaag atttaattca
    13381 ttggaaaaag gatggcgaag cagcaaaact tgttcctcaa gaaggatatg ataagaatga
    13441 ttggcgcgat ccttttgcaa tttttgatca agatcatgga aaatacgttt tgatactagg
    13501 agctagaaaa ccagcaagta agaaaaagcc gacggggaga ttggtctatt ttgaatccga
    13561 agatttaaaa gattggaatt tcaaaggaga ctactgggct aataatgaat ttaacatgat
    13621 tgaaatgcct gatatatttg aaattgatgg aacgtggtat ttactttttt cagagtattg
    13681 tgaagataaa aagactaaat atcgagtcgg agcaggatta ttttcagatt ggaaatcacc
    13741 agtagacgaa gctttcgacg gaaaagccta ttatgcagca agaacagtcg gaactaatga
    13801 aactggccgt tttctaactg gctgggtggc aacaaaagaa ggggagaacg atcaaaataa
    13861 ctgggattgg ggcggtgcac ttgttccaca tcaaattatt caaaagacag acaaaactct
    13921 tggtgttaaa ctgcctgaaa aaataattga gaattttaaa gaaatcactc cattttcaag
    13981 ttttaagtta gagaaagaat ttggaaaaaa agaaagagta attgttccta aaatcgaaga
    14041 ttattttcta tttgaaacaa ctttgactat tacatcgtta acaaaggagt ttgctttaaa
    14101 attatttgta gatagtgata ctcaagaagg atacgaattt cgtatttcat tgattaataa
    14161 tcagcttact ttcggaaaaa ctccgaacta tccttggcct caaatgtttg ataaggggct
    14221 gcaacgacca ctatttttgg aaataggcaa agaatatgaa ctaaagttaa ttgttgatga
    14281 tacgatagct gttttatata ttgataatgt tgctttaagt actagaatgt atgataaagt
    14341 cggaacagca ttaagtatga ctgttacgga gggagcaatc aaggtagata aactagacta
    14401 ttcaataaat tatgaatgtg gattttaaaa caattgaagg gatgatgtaa gtgaaattaa
    14461 atggtttaca acatattgga attccaacaa aagattttgg agcttcaaaa atcttttatg
    14521 aatccttagg gtttagtctg attaatgaag aaatgaatgt gggaagtaaa gtcggctttt
    14581 tcgagttgaa tggaacgata atagaaatat gggaagataa aactactgag tgtagaggag
    14641 ccattgatca tctagcatta gatacggatg acatagatga ggtattcaat tcaattcaag
    14701 atttaggata tgaattatta gataaaaata ttatggaatt accgttctgg gaaaatggaa
    14761 tccgttactt taatttctac ggaccaaatc atgagattat agagataagc caaatgaaga
    14821 aataaataga taacgaagtg ctctaagttg gtatgaacga aaagtacttc attttttggt
    14881 atcagcattt caatagttta atgagaattt actatttcaa attaattcaa agtgacagta
    14941 ttttttactt atttggtttc aaattttgta atccttccta tttattggaa tacactccac
    15001 attttgtata caagtcatat catgttttag caaagctctt accaagcaaa gttataatga
    15061 tgttttccaa agtttgatct ttcgatggag gctaacacat attttttgca ggacagccaa
    15121 aattagatgt atagaataga gtctgtgagg cgtatagaca gtttattgtc tgtatgcctt
    15181 tttttatttt gaaaaatttt tagtattttt cgaaaaatgc tcactcctag
//
LOCUS       DACEEZ010000068        14899 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20313_2017
            SAMN12639966-rid9879223.denovo.068, whole genome shotgun sequence.
ACCESSION   DACEEZ010000068 DACEEZ010000000
VERSION     DACEEZ010000068.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639966
            Sequence Read Archive: SRR10023158
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 14899)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14899)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741683.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741683.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:44:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,016
            CDSs (total)                      :: 2,962
            Genes (coding)                    :: 2,889
            CDSs (with protein)               :: 2,889
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 36 of 73
            Pseudo Genes (incomplete)         :: 29 of 73
            Pseudo Genes (internal stop)      :: 18 of 73
            Pseudo Genes (multiple problems)  :: 8 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14899
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639966-rid9879223.denovo.068"
                     /isolate="E20313_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            <1..500
                     /locus_tag="IWO89_002100"
     CDS             <1..500
                     /locus_tag="IWO89_002100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS6 family transposase"
                     /protein_id="HAQ0456826.1"
                     /translation="WKKKNRQSFYSWKMDETYIKIKGRWHYLYRAIDADGLTLDIWLR
                     KKRDTQAAYAFLKRLHKQFGEPKAIVTDKAPSLGSAFRKLQSVGLYTKTEHRTVKYLN
                     NLIEQDHRPIKRRNKFYQSLRTASSTIKGMETIRGIYKKNRRNGTLFGFSVSTEIKVL
                     MGITA"
     gene            665..1046
                     /locus_tag="IWO89_002101"
                     /pseudo
     CDS             665..1046
                     /locus_tag="IWO89_002101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303242.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            1177..2127
                     /locus_tag="IWO89_002102"
     CDS             1177..2127
                     /locus_tag="IWO89_002102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002821555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HAQ0456827.1"
                     /translation="MLKTYSLTKDTLLVSSESNIKNSSVVYVYGDEREYIESSEHKYD
                     FKIGNILTFDDRVAYDTMIDKNDEKSLLVSFLFPEVHEGGHLDTLTTSVVFLVFKNKL
                     IIFTKQKLKFIPELLSNMVLRDVNHFVEEVLLKIMQKDFYMMLNDLRGVKEKIDDLDS
                     EISTSGSLKPTFGDLLTLQKYMIALSTTYKANHKALDFIKKNFTTIDDIDFSTKKIID
                     DLYDTSDTMDRIILSYSQYLDKLENMINNMISYQLNMIMKTLTEISIVLTIPAIIFGF
                     WGINVHVPFEKSSYGVLAVLIISAILSLICWLLMRRKTYL"
     gene            2645..3100
                     /locus_tag="IWO89_002103"
     CDS             2645..3100
                     /locus_tag="IWO89_002103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HAQ0456828.1"
                     /translation="MVQQIVLPIKDSSVLKMVQDTLLDSFRAGRRNYTVFQVGKATLL
                     RVSDVMTLKKSDVYNPDGSVKNATFIHDKKTGKANTLYLKPVQQDLLQYHDWLAQENI
                     NSEWLFPSTAHYDLHITEKQFHKVMAHVGDLLGINLEDKEKKLRVRKPF"
     gene            3411..3767
                     /locus_tag="IWO89_002104"
                     /pseudo
     CDS             3411..3767
                     /locus_tag="IWO89_002104"
                     /inference="COORDINATES: protein motif:HMM:NF013878.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            3780..4190
                     /locus_tag="IWO89_002105"
                     /pseudo
     CDS             3780..4190
                     /locus_tag="IWO89_002105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001812298.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
     gene            4305..4469
                     /locus_tag="IWO89_002106"
     CDS             4305..4469
                     /locus_tag="IWO89_002106"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0456829.1"
                     /translation="MSYPRTDTPFITENEFAYLKANFGKYSGFLGLDLEMLLSLIMIR
                     SNNSTQILTN"
     gene            complement(4620..5599)
                     /locus_tag="IWO89_002107"
                     /pseudo
     CDS             complement(4620..5599)
                     /locus_tag="IWO89_002107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332307.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
     gene            complement(5604..6371)
                     /locus_tag="IWO89_002108"
     CDS             complement(5604..6371)
                     /locus_tag="IWO89_002108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007209218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HAQ0456830.1"
                     /translation="MNKKRRLEKILDMLKIDGTITIKEIIDELDISDMTARRDLDALE
                     ADGLLTRTHGGAQLLSSKKPLEKTHIEKKSLNTKEKIDIAKKACSLIKDGDTIFIGPG
                     TTLEQLALELKGRKGYKIRVITNSLPVFLILNDSETIDLLLLGGEYREITGAFVGSMA
                     STNLKAIRFAKAFVSANAVTHNSIATYSDKEGVIQQLALNNAVEKFLLVDSTKFDRYD
                     FFNFYNLDQLDTIITDNQISPQHLEEFSQYTTILKAD"
     gene            6861..7286
                     /gene="lacA"
                     /locus_tag="IWO89_002109"
     CDS             6861..7286
                     /gene="lacA"
                     /locus_tag="IWO89_002109"
                     /EC_number="5.3.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005237726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-6-phosphate isomerase subunit LacA"
                     /protein_id="HAQ0456831.1"
                     /translation="MAIVVGADLKGTRLKDVVKNFLVEEGFEVIDVTKDGQDFVDVTL
                     AVASEVNKDEQNLGIVIDAYGAGPFMVATKIKGMVAAEVSDERSAYMTRGHNNSRMIT
                     VGAEIVGDELAKNIAKGFVNGKYDGGRHQIRVDMLNKMC"
     gene            7303..7818
                     /gene="lacB"
                     /locus_tag="IWO89_002110"
     CDS             7303..7818
                     /gene="lacB"
                     /locus_tag="IWO89_002110"
                     /EC_number="5.3.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002994294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-6-phosphate isomerase subunit LacB"
                     /protein_id="HAQ0456832.1"
                     /translation="MRIAIGCDHIVTDEKMAVSEFLKSKGHEVLDFGTYDHARTHYPI
                     YGKKVGEAVVSGQADLGVCICGTGVGINNAVNKVPGVRSALVRDMTSAIYAKEELNAN
                     VIGFGGKITGGLLMNDIIEAFIEAEYKPTEENKKLIAKIEHVETHNAHQADEDFFTEF
                     LERWDRGDYHD"
     gene            7829..8761
                     /gene="lacC"
                     /locus_tag="IWO89_002111"
     CDS             7829..8761
                     /gene="lacC"
                     /locus_tag="IWO89_002111"
                     /EC_number="2.7.1.144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-6-phosphate kinase"
                     /protein_id="HAQ0456833.1"
                     /translation="MILTVTLNPSVDISYPLETLKIDTVNRVKDVSKTAGGKGLNVTR
                     VLYESGDKVTATGFLGGKIGEFIESELEQSPVSPAFYKISGNTRNCIAILHEGNQTEI
                     LEQGPTISHEEAEGFLDHYSNLIKQSEVVTISGSLPSGLPNDYYEKLIQLASDEGVAV
                     VLDCSGAPLETVLKSSAKPTAIKPNNEELSQLLGKEVTKDIEELKDVLKESLFSGIEW
                     IVVSLGRNGAFAKHGDVFYKVDIPDIPVVNPVGSGDSTVAGIASALNSKKSDADLLKH
                     AMTLGMLNAQETMTGHVNMTNYETLNSQIGVKEV"
     gene            8764..9744
                     /gene="lacD"
                     /locus_tag="IWO89_002112"
     CDS             8764..9744
                     /gene="lacD"
                     /locus_tag="IWO89_002112"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase"
                     /protein_id="HAQ0456834.1"
                     /translation="MVLTEQKRKSLEKISDKNGVISALAFDQRGALKRLMAQYQDTEP
                     TVAQMEELKVLVADELTKYASSMLLDPEYGLPATKALDKEAGLLLAYEKTGYDTSSTK
                     RLPDCLDVWSAKRIKEQGADAVKFLLYYDVDSSDELNQQKQAYIERVGSECVAEDIPF
                     FLEILAYDEEISDAGSVEYAKVKPRKVIEAMKVFSDPRFNIDVLKVEVPVNVKYVEGF
                     ADGEVVYSKAEAADFFKAQEEATNLPYIYLSAGVSAKLFQETLQFAHDSGAKFNGVLC
                     GRATWAGSVEPYIKEGEKAAREWLRTTGFENIDELNKVLVKTASPWTDKV"
     gene            9772..10089
                     /locus_tag="IWO89_002113"
     CDS             9772..10089
                     /locus_tag="IWO89_002113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011669100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HAQ0456835.1"
                     /translation="MNREEMTLLGFEIVAYAGDARSKLLEALKAAENGDFAKADSLVV
                     EAGSCIAEAHNSQTAMLAREASGEELPYSVTMMHGQDHLMTTVLLKDVIHHLIELYKR
                     GAK"
     gene            10089..11795
                     /locus_tag="IWO89_002114"
     CDS             10089..11795
                     /locus_tag="IWO89_002114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAQ0456836.1"
                     /translation="MHKLIELIEKGKPFFEKISRNIYLRAIRDGFIAGMPVILFSSIF
                     ILIAYVPNAWGFHWSKDIETFLMTPYSYSMGILAFFVGGTTAKALTDSMNRDLPATNQ
                     INFLSTMLASMVGFLLMAAEPAKEGGFLTAFTGTKGLLTAFIAAFVTVNVYKVCVKNN
                     VTIRMPEEVPPNISQVFKDLIPFTVAVVLLYGFELIVKGTLGVTVAESIGTLLAPLFS
                     AADGYLGITLIFGAYAFFWFVGIHGPSIVEPAIAAITYANIDANLHLIQAGQHADKVI
                     TSGTQMFIVTMGGTGATLIVPFLFMWICKSERNRAIGRASVVPTFFGVNEPILFGAPI
                     VLNPIFFVPFIFAPIVNVWIFKFFVDTLNMNSFSANLPWVTPGPLGIVLGTNFQVLSF
                     ILAGLLVVVDTIIYYPFVKVYDEQILEEERSGKTNDALKEKVAANFNTAKADAVLGKA
                     GVAKEDVAANNNITKETNVLVLCAGGGTSGLLANALNKAAAEYNVPVKAAAGSYGAHR
                     EMLPEFDLVILAPQVASNFDDMKAETDKLGIKLAKTEGAQYIKLTRDGQGALAFVQQQ
                     FD"
     gene            11937..13343
                     /gene="lacG"
                     /locus_tag="IWO89_002115"
     CDS             11937..13343
                     /gene="lacG"
                     /locus_tag="IWO89_002115"
                     /EC_number="3.2.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-galactosidase"
                     /protein_id="HAQ0456837.1"
                     /translation="MTKTLPKDFIFGGATAAYQAEGATHTDGKGPVAWDKYLEDNYWY
                     TAEPASDFYHKYPVDLELAEKYGVNGIRISIAWSRIFPTGYGEVNEKGVEFYHNLFAE
                     CHKRHVEPFVTLHHFDTPEVLHSDGDFLNRENIEHFVDYAAFCFEEFPEVNYWTTFNE
                     IGPIGDGQYLVGKFPPGIQYDLAKVFQSHHNMMVSHARAVKLYKDKGYKGEIGVVHAL
                     PTKYPYDPENPADVRAAELEDIIHNKFILDATYLGHYSDKTMEGVNHILAENGGELDL
                     RDEDFQALEAAKDLNDFLGINYYMSDWMQAFDGETEIIHNGKGEKGSSKYQIKGVGRR
                     IAPDYVPRTDWDWIIYPEGLYDQIMRVKNDYPNYKKIYITENGLGYKDEFVDNTVYDD
                     GRIDYVKKHLEVLSDAIADGANVKGYFIWSLMDVFSWSNGYEKRYGLFYVDFDTQERY
                     PKKSAHWYKKVAETQVIE"
     gene            13366..13638
                     /locus_tag="IWO89_002116"
     CDS             13366..13638
                     /locus_tag="IWO89_002116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007209209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3884 family protein"
                     /protein_id="HAQ0456838.1"
                     /translation="MLSDTLEKVQHLFRPGFEKVYIVSFEDCPMIPELEATPLLKCGK
                     WYVSTGKEWICHSDLELSAFEWEFLQSLDVEIRETIHFEVNYLPFQ"
     gene            13659..14558
                     /locus_tag="IWO89_002117"
     CDS             13659..14558
                     /locus_tag="IWO89_002117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002889672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose 1-epimerase family protein"
                     /protein_id="HAQ0456839.1"
                     /translation="MTIELKNEYLTVQFKTLGGQLTSIKDKDGIEYLWQADPNYWNGQ
                     APILFPICGSLRNDWAIYRPQERPFFTGLIRRHGFVRKEEFILEEVNDNSVTFSIKPN
                     AEMLDNYLYQFELRVVYTLNGKSIRTEFQVTNLEMEKTMPYFIGAHPAFNSPLVEGEN
                     YEDYYIEFSEVESCSIPKSFPETGLLDLQDRTPFLENQKILDLDYSLFSHDAITLDQL
                     KSRSVTLRSRKSGKGLRVDFDDFPNLILWSTTNKSPFIALEPWSGLSTSLEEGNFLED
                     KRQVTKISPQETSRKIYDITILN"
BASE COUNT         4875 a         2467 c         2882 g         4675 t
ORIGIN      
        1 tttggaagaa gaaaaataga caatccttct attcatggaa aatggacgaa acctatatca
       61 aaattaaggg acgttggcat tatctttatc gtgcaattga tgcggacggc ttaaccttag
      121 atatctggtt acgaaagaaa cgggatacgc aagcagccta tgctttctta aaacgactcc
      181 ataaacagtt tggtgagccg aaagcaattg tgaccgataa agcaccttct cttggctccg
      241 cctttagaaa gttacagagt gtgggtttat atactaagac agagcaccga actgtgaagt
      301 atcttaacaa tttaatagaa caagaccatc gacctattaa acgacgaaat aaattttatc
      361 aaagtctccg tacagcctct tccacgatta agggcatgga gaccattcga ggaatatata
      421 aaaagaaccg aagaaatgga acgctcttcg gcttttcggt gtctactgaa atcaaggtat
      481 taatgggaat aacagcctaa gatatttgga gttcagagag ggtgcgtttg attttcaaac
      541 ttcgcaacag aaccgatgac actatcgttc ttcatcaacg taagttagat ttgttgaaag
      601 agcagaaaaa aggatttcta caaaaaatgt ttgtttgata ttttaaatag aaaagagaga
      661 taaagtgaag tttttaaatt ttttgaacta taaatattta aactgggtat tatatggtat
      721 tacattattt tttattattt attacttttt taccgatgat gatatttttt taattttagc
      781 aatacttttt cttgtttcat ctaattttag tgataactta attgaagttc ttacagaacc
      841 agaaattcgg tctatgacgg atgaagaaca acagattatt aaagctatga tttcggaaaa
      901 taagaaagac gtagtagtta tcaaaaagat tagagagtat acaaatttag gtgttttaca
      961 agccaaaaaa gtatatgaaa aaaaaaaaaa gggtctataa ttacaaatta gataatacat
     1021 tagaagctgg agacaagatt gtctagataa tgtgtgagat aaaatttttg gcaggttatc
     1081 atacataaga caaaatgttt ttaaaactga taacgtattt attccacaac aattacgcat
     1141 ttatttctga gaaaaatata aagcaggagg attacaatgc tgaaaactta ttccttaaca
     1201 aaagatacat tattagtttc gtcagaatca aatatcaaaa attcttctgt tgtttatgtt
     1261 tatggtgacg aacgagaata tatcgaatct tctgaacata aatatgactt taaaataggc
     1321 aatatactaa cgtttgatga tcgagttgca tatgacacga tgatagataa gaacgatgaa
     1381 aaaagtttgc tcgtaagttt tttgtttccc gaggtacatg aaggtgggca tctagatacc
     1441 ctgacgacat ctgttgtttt cttggtattt aaaaataagt tgataatttt tacaaaacaa
     1501 aaattaaaat tcattcccga actcttatcc aatatggttt tacgtgatgt caatcatttc
     1561 gtggaggaag tacttttaaa gatcatgcaa aaagatttct atatgatgct taacgacctt
     1621 cgtggagtca aggaaaaaat tgatgattta gactcagaaa tatcaacgtc cggttcactc
     1681 aaaccaacgt ttggtgattt attgactctt caaaaataca tgatagcttt gtcaacaact
     1741 tataaagcta accataaagc gcttgatttc attaaaaaaa atttcacaac tattgacgat
     1801 attgatttta gtactaaaaa aataattgat gatttatacg atacaagtga tacaatggat
     1861 agaatcattt taagttacag ccaatatttg gacaaattgg aaaatatgat aaacaatatg
     1921 atttcgtatc agctaaacat gattatgaaa accctaaccg agatatcaat tgttttgact
     1981 attccagcaa ttatttttgg cttttgggga atcaatgtcc atgtcccgtt tgaaaagtct
     2041 tcttatggtg ttttagccgt attgattatc tctgctatac taagtctaat ttgctggctt
     2101 ttgatgcgac gcaaaacata tttgtaaagt atttgtgata ccctaatatg atttaaagca
     2161 gaatttcttt gttgactttt taaaaggctg attcctttca ataatttaat agcttcagcg
     2221 gctgctcaga agtcaataaa ttggactatt tttattcagt tacttaggca acatttgaga
     2281 tacgattgcg attataaaaa ttgatatacc aaccaatggc tgcttggaag actgacatat
     2341 tagcctcgat atcatcgaat aactaaatac agaaaaatac aataaatatt atcaaactag
     2401 ggactatact agaaaacatc agtacattcc ttaactcagg gtctataatt agaaaatcgc
     2461 ccctttgaaa actgattaaa acagccccca ttatctagtg actagataat gggggctgtt
     2521 tttttgttaa gtgatataat aaaccataaa agaagtttag aaatacggtc ctggtcatca
     2581 ataaataaac aatttaatta attatttatt taattattta atttcagaaa ggtatacatg
     2641 aatcatggtg caacaaattg tcctacccat caaagactcc agcgtcttaa agatggtaca
     2701 agatacactt ctcgatagtt tccgtgctgg tcgccgaaat tacaccgttt ttcaagttgg
     2761 taaggccacg ctactacgtg tgagtgatgt catgacatta aaaaaatcag atgtctataa
     2821 tccagacggc tctgtcaaaa atgcaacctt tattcatgat aaaaagaccg gtaaagcaaa
     2881 cacgttatat ttaaagcctg ttcagcaaga cttgttgcaa tatcatgatt ggctggccca
     2941 agaaaatatc aattctgagt ggttatttcc ctcgacagcc cattatgatc ttcatatcac
     3001 tgagaagcag tttcataagg tgatggcgca tgttggtgat ctcttaggaa tcaacttgga
     3061 agacaaagag aaaaaattgc gagtcagaaa accgttttga gatatgaaca gcatttgcct
     3121 tcacgcctta gcgattttaa ataatagcct tgaaagtgat tatggaaaag ccctctacgc
     3181 tcaatgcaaa attgacgtag aaactatttt tgatcgattg aaaggtgtat ttggtatgtg
     3241 aagaacccat gtcagaggca agaacgttgc acctactgac attggaatga tgcttatgag
     3301 catgaatcta accaaattat cccttgaggc tagaagaaaa gcggaagctt ttcaacacaa
     3361 agcagccaaa aataaaaaac gcgacgaagc aatcacattt atgattattc atcgcgtttt
     3421 ttatatttag agctagttat ttgctagacc cactatttac agatgaaacg tttatcacct
     3481 ttggttttgg gcatttagtg gaattagcag aaccaggtca ttatgacgaa aagtggcaaa
     3541 attggaaact tgaatctttg ccgatttttc ctgatcgata cgattttgaa gttgcaaaag
     3601 ataagggaaa gcagtttaaa attgttgcag aacttctcaa aaaggcaaat acaattattg
     3661 ttgcaacaga tagcgacaga gaaggtgaaa atatcgcctg gtcgattatc cataatgcaa
     3721 atgccttttc aaaagataaa acatttaaaa gactatggat caatagctta gaaaaagatg
     3781 tgatccgaag cggttttcaa aatttgcaac caggaatgaa ttactatccc ttttatcaag
     3841 aagcgcaaac acgccaaatt gccgattggt tgatcggcat gaacgcaagc cctttgtata
     3901 cgttaaattt acaacagaag ggcgtacaag gtacattttc actaggacgt gttcaaacgc
     3961 ccaccttata ccttattttt cagcgccagg aagccataga gaattttaaa aaagaacctt
     4021 ttttcgaggt ggaagctaag taaaacaagg gtcatttaag ggcgttctaa gccccacaca
     4081 gcgttttaaa acccaagagg agctttttag cttttgtttc ttctaaacaa gctaaaatag
     4141 gcaatcaaga ggggataatt gctgatgttc aaaccaaaga gaagaaaacg aatagtccga
     4201 gttcgttttc tttaagtagt ttgcaatcaa aagtcaatca gctttataaa gcgacagcga
     4261 gccaaacttt aaaagctatg caaggactgt atgaagcaaa attattgagt tatccaagaa
     4321 cagatacacc atttattaca gagaacgaat ttgcttattt aaaagcgaat tttggcaaat
     4381 atagcggttt tttaggactt gatcttgaaa tgttgttatc attgataatg atacgttcaa
     4441 acaacagcac ccaaattttg acgaattagt gaaactctat gaaaaagacg tagtaaaaca
     4501 tgcaacatcc tattctaatc agctggtaaa gcttaattag gttatataag cttgaaaata
     4561 aagggtttgt ccaatatgga caagcctttt tcttatgttt tgccacagat ttgccacact
     4621 tattgtgtaa gagtatcaag gataggtata atttgatact ttgttttttt agtgatgtgc
     4681 aaataaattt ctgttacacg actacctcta gaatgcccta cacggtcttg aattgcttct
     4741 aggggtattc cttactctgc taagaaacta atatgcgtat gacggaatat atgggtcgta
     4801 atatgtttat taaaactaag tttattatca atatcaattc ctgctcgttt tggattacct
     4861 tttcgattta attttccatt ttcaggtgag ttgaggaaat tgttaaggac actataatga
     4921 agaggtgttc cattatagtc gggtttatga cgacttttgg aagtaaaaat ccatccatta
     4981 tctttataat caggattgaa tctcatatgt tggtgattta actcaatttc ctctttaaga
     5041 atttctatag cccttttaga taattggaca tttcggtaac tttgtaagtt ttttacatta
     5101 ccttcatagc tattttctgc agctcctccg tgagtggact gactaatgat gatattttgc
     5161 ttttcaaaat attgataggc accaatattt tggagtttcg actctgtaag tcaacagggc
     5221 cattagttag aatacctatt gggatgcggt ttttatgtag agccagcagt agttgatgta
     5281 tctcaggaat catcgtaatc tgatcgagtt catattcata gtcagcttgg aagtctagag
     5341 cttctgtcct agaaatacta tcgatgccaa attcttccat agtcttctga cagcgaaaaa
     5401 ttctaagctg ctcaatactg atgagttttt tcgtgtattt aggaaaagca acatcagacc
     5461 aatatctgaa acgtttgtaa atgttatcta tttgttggat atcaatcgtt ggaaaacatt
     5521 tctcaacgct acgtttaaag gggagttgat ggtcataaag agtgtcatca aggtcaaaaa
     5581 caatattttt ataagtcata attctagtcc gcttttaaaa tagtagtgta ctggctaaat
     5641 tcctctaagt gttgagggct aatctggtta tctgtaatga tggtatcgag ttgatctaga
     5701 ttatagaagt taaagaaatc gtatcgatcg aatttagtac tgtctactaa taagaatttt
     5761 tctacagcat tgtttagggc aagttgttga atcacacctt ccttgtcact atatgtagca
     5821 atagaattat gggtaacagc atttgcacta acaaaagctt tggcaaatct aattgctttt
     5881 aaatttgtcg aagccattga acctacaaaa gctccagtta tttctctata ttcaccgcca
     5941 agaagcaata aatcaatggt ttcgctatca tttagaatca agaacacagg gagactattt
     6001 gtaatgacac gaattttata acctttacga cctttcaatt ctaatgccag ttgttcaagt
     6061 gtagttccag gtccaataaa aatagtatcg ccatctttga ttaaagagca ggctttttta
     6121 gcaatgtcaa ttttttcttt tgtatttaga cttttcttct cgatatgtgt cttttcaagt
     6181 ggctttttag aggaaagcaa ttgtgcacca ccatgagtac gtgttaaaag tccatcagct
     6241 tctagagcat caaggtctct acgggctgtc atatcggaaa tatctagttc atctattatt
     6301 tcttttatgg ttatggtccc atcaatcttt aacatatcta aaattttttc taatcgtcgt
     6361 tttttgttca tatgaagacc cttctttcat aaagtaattt ttttccaaag ataattctct
     6421 tttaattgta tcataaaaga caatattttc aaggtaaaac aaacaatttc aaacaaaaac
     6481 aaatgttaga tgatgaaata agaacagagg attgacgtat attagcttag gtcagatttt
     6541 gtataagacg aaaataaagt aggacctctt aatcagtaag ttatagaaag taaaagactt
     6601 ttgtaatact tgaatagata tttcacgtcc attttgtgat ggattaaatg aacaaaaatg
     6661 aacaataatt taacggtgtt atctattttt taaaaaaaca aataaaaaaa acaaaaaata
     6721 aacaaaaaaa gtgttgcttt ttgtttgaat gcttgatatt atataaacaa agaaatgatg
     6781 aaaacgtttt ttcgaacatt ttgcaaaatg ttttctactt ctacgtagca tttctttcta
     6841 aaatttagga ggtggtcaaa atggctattg ttgttggtgc agatctcaaa ggtacgagat
     6901 taaaagatgt tgtgaaaaat tttcttgtag aagaaggttt tgaggtaatt gatgtgacta
     6961 aggacggaca agattttgtt gatgttacct tggcagttgc ctctgaagta aataaagatg
     7021 agcaaaactt aggtattgta attgatgctt atggagctgg tccattcatg gtagcaacta
     7081 aaattaaagg tatggttgca gcagaagttt cagatgaacg ctcagcttat atgacacgtg
     7141 gacataacaa ttcacgaatg attacggttg gcgctgaaat cgtcggtgat gagcttgcta
     7201 aaaacatcgc taaaggtttc gtcaatggta aatatgatgg cggacgtcat caaatccgag
     7261 tagatatgct taacaaaatg tgttaagaaa aggagttaag gaatgagaat cgcaattgga
     7321 tgtgaccaca ttgtcacaga tgaaaaaatg gcagtatcag aattcttgaa atcaaaagga
     7381 catgaggtcc ttgatttcgg gacatatgac cacgcacgta ctcactaccc tatctatggt
     7441 aaaaaagtcg gtgaagcagt agtaagtggt caagcagact tgggagtatg tatctgtggt
     7501 acaggtgtcg gcattaacaa tgcagtcaat aaagttccag gtgttcgctc agcgttggtt
     7561 cgtgatatga catcagctat atatgcaaaa gaagagttaa atgcgaatgt tatcggtttc
     7621 ggtggaaaga ttactggtgg tcttcttatg aatgacatca ttgaagcttt cattgaagct
     7681 gagtacaaac caacagaaga aaataaaaaa ttgattgcaa aaatcgaaca cgttgaaaca
     7741 cataatgcac atcaagcaga tgaagatttc tttacagaat tccttgaaag atgggaccgt
     7801 ggtgactacc acgattaagg tataacaaat gattctgaca gtcacactaa atccttcagt
     7861 agacatctct tatcctctag aaacattgaa aattgataca gtgaataggg tgaaagatgt
     7921 tagtaagaca gctggtggaa aaggcttaaa tgttactaga gtcttgtatg aatctggtga
     7981 taaagttact gctacaggtt tcttaggtgg taaaattggt gaatttattg aaagcgaatt
     8041 agaacaatct cctgttagtc cagcttttta taaaatctca ggaaacacta gaaactgtat
     8101 tgctatctta cacgagggta accaaacaga aattttggaa caaggaccaa caatttctca
     8161 tgaagaagca gaaggctttc ttgaccacta tagcaatctt attaaacaat cagaagttgt
     8221 gacaatttca ggaagtttac cttcaggact tcccaacgat tattatgaaa aacttatcca
     8281 acttgcttcg gatgaaggag tagcagtcgt tttggattgt tcaggtgcac ctcttgaaac
     8341 agttctaaaa tcgtcggcta aacctactgc catcaaacct aataatgagg aactttcaca
     8401 gttgcttgga aaagaagtaa caaaagatat tgaagaactc aaagacgttc ttaaagagtc
     8461 actttttagt ggtatcgaat ggatagtggt ctcattaggt cgaaacggtg cctttgcaaa
     8521 acatggtgat gttttctata aggtagatat tcctgatatc ccagttgtta atccagtcgg
     8581 atcaggggac tcaacggtag ctggtattgc atcagcttta aatagtaaaa aaagtgacgc
     8641 tgacttatta aaacatgcga tgacattggg tatgttaaat gctcaagaaa caatgacagg
     8701 gcatgtgaat atgactaatt acgaaacact aaactcacaa attggagtaa aagaggtata
     8761 aaaatggtac ttacagaaca gaaacgcaaa tctttggaaa aaatttcaga taaaaacggt
     8821 gttatctcag ctttggcatt tgaccaacgt ggtgctttga aacgtttgat ggcacagtat
     8881 caagatacag aaccaactgt agctcaaatg gaagaactaa aggttttggt tgctgatgaa
     8941 cttacaaaat acgcttcttc aatgttgctt gaccctgaat atggacttcc agcaacaaaa
     9001 gcattggata aagaagcagg tcttcttctt gcctatgaaa aaacaggtta tgatacatca
     9061 agtacaaaac gtttgcctga ctgcttggat gtttggtctg caaaacgtat caaagagcaa
     9121 ggcgctgatg ccgttaagtt cttgctttac tatgatgtag acagctcaga tgaacttaac
     9181 caacaaaaac aagcttatat tgaacgtgtt ggttctgaat gtgtcgctga agatattcct
     9241 ttcttcttgg aaatccttgc ttatgatgaa gaaatctcag atgcaggttc tgttgaatat
     9301 gcaaaagtga aacctcgcaa agtcattgaa gcgatgaaag ttttctctga tccacgcttt
     9361 aacattgatg ttcttaaagt agaagtacca gttaacgtta aatatgtcga aggttttgct
     9421 gatggtgaag tagtttacag taaagctgaa gctgctgact tctttaaagc acaagaagaa
     9481 gcgactaacc ttccatacat ctacctaagt gcaggtgtat ctgctaaatt gttccaagaa
     9541 acattgcaat ttgctcacga ctcaggtgcc aagtttaatg gtgtgctttg tggacgtgct
     9601 acttgggcag gatctgtaga accttacatc aaagaaggtg aaaaagcagc acgtgaatgg
     9661 ttgcgtacta ctggattcga aaatattgat gaacttaaca aagttcttgt taaaacagct
     9721 agtccatgga ctgataaagt atagtaaaaa cataaaacgg aggatattgt tatgaacaga
     9781 gaagagatga ctctcttagg gtttgaaatt gttgcttatg ctggagatgc tcgctctaaa
     9841 cttttagaag cgcttaaagc ggctgaaaat ggtgatttcg ctaaggcaga tagtcttgta
     9901 gtagaagcag gaagctgtat tgcagaggct cacaattctc agacagctat gttggctcga
     9961 gaagcttctg gggaggaact tccatacagc gttaccatga tgcatggtca ggaccactta
    10021 atgactactg tcttattaaa agatgtgatt catcacctca tcgaacttta taaaagagga
    10081 gcaaaataat gcataaactc attgaactta ttgagaaagg gaaaccgttc tttgagaaaa
    10141 tctctcgaaa catctatctt cgtgctattc gtgatggatt tatcgctggt atgccagtca
    10201 tccttttctc atctatcttt atccttattg cctatgtccc aaatgcttgg ggattccact
    10261 ggtcaaaaga cattgagacc ttcttgatga ctccttatag ctattcgatg ggtatcctag
    10321 cattctttgt tggtggtact accgctaaag ctttgacgga ctcaatgaac cgtgacctac
    10381 ctgcgaccaa tcagattaac ttcttatcta cgatgctagc gtctatggta gggttccttt
    10441 tgatggcagc tgagcctgca aaagaaggag gcttcttgac agccttcacg ggaacaaaag
    10501 gtcttttgac agcatttatt gcagcctttg tgacagttaa tgtttacaag gtctgtgtga
    10561 aaaataatgt tactattcgt atgcctgaag aggttccacc aaatatctct caagtattta
    10621 aagacttgat tccattcact gtagcggttg ttctccttta tggatttgaa ctcattgtta
    10681 agggaactct tggtgtaacc gttgcagaat caatcggtac ccttcttgct cctctcttct
    10741 cagctgcaga tggttacctt ggaattacac ttatctttgg tgcttatgcc ttcttctggt
    10801 ttgttggtat ccacggtcct tcaattgtcg aaccagcaat cgctgctatc acttatgcca
    10861 atatcgatgc caacttgcat cttatccaag ctggacaaca tgcagacaaa gttatcactt
    10921 ctggtactca aatgtttatt gttaccatgg gtggaacagg agctacattg attgttccat
    10981 tcttgttcat gtggatttgt aaatcagaac gtaaccgtgc catcggacgt gcctcagttg
    11041 ttccaacatt ctttggggtt aatgagccaa tcttatttgg tgcaccaatc gtattgaatc
    11101 cgattttctt tgtaccgttc attttcgctc cgattgtcaa cgtttggatc tttaaattct
    11161 ttgttgacac attgaacatg aactcattct ctgccaacct tccatgggtt actcctggtc
    11221 cgttgggaat tgtactcggt actaacttcc aagttctatc atttatctta gccggactct
    11281 tggtagttgt tgatactatc atttattacc catttgttaa ggtatacgat gaacaaattc
    11341 ttgaagaaga acgttctggt aaaacaaacg atgctcttaa agaaaaagta gctgcaaact
    11401 tcaatactgc taaagcagat gccgttcttg gaaaagcagg cgttgctaaa gaagatgtag
    11461 cagcaaacaa taatatcaca aaagaaacta atgtccttgt tctttgtgca ggtggaggta
    11521 caagtggctt gcttgccaat gccttgaaca aagcagcagc agaatacaat gttccagtca
    11581 aagcggcagc gggtagttac ggtgctcacc gtgaaatgtt gccagagttt gacttggtta
    11641 tcttggctcc acaggtcgct tcaaacttcg atgatatgaa ggctgaaacg gataaattgg
    11701 gcattaaact tgcaaaaact gaaggagctc aatatatcaa attgacacgt gatggacaag
    11761 gtgctcttgc atttgttcaa caacagtttg attaataaat ttgtaaaagt tatttgagac
    11821 gagattattc tttcaattcc ttataatatt gttaaccata tcattgcatt tcatttcctt
    11881 ggtttccgga actcagaata tcgtctcaaa tgcttttttt gaaaggattt atagttatga
    11941 ctaaaacact tcctaaagat tttatttttg gtggtgctac agctgcttat caagcagaag
    12001 gtgcgactca cacggatggt aaaggcccag ttgcatggga taagtatctt gaagacaatt
    12061 attggtacac agcagagcct gcaagtgatt tttaccataa atatcctgtt gatttagaat
    12121 tagctgaaaa atatggcgtt aatggtattc gtatttcaat tgcctggtct cgtattttcc
    12181 caactggcta tggcgaagta aatgaaaaag gtgttgaatt ctaccataat ctttttgccg
    12241 agtgccacaa acgtcatgtt gagccattcg tgactttgca ccactttgac acaccagaag
    12301 ttcttcactc agatggagat ttcttaaacc gcgaaaatat agaacacttt gtagattatg
    12361 ccgctttctg ttttgaagag ttcccagaag taaactactg gacaactttc aatgaaattg
    12421 gcccaattgg agatggtcaa tacttggttg gtaaattccc tccaggcatt caatatgatc
    12481 ttgccaaagt cttccaatca caccacaata tgatggtttc tcatgcacgt gctgtgaaat
    12541 tgtataaaga taaaggttat aaaggcgaaa ttggtgtagt acatgcgttg ccaaccaaat
    12601 acccttatga tccagaaaat ccagcagatg ttcgtgccgc tgagttagaa gatatcatcc
    12661 ataacaaatt tatcttggat gcaacctacc ttggacacta ttcagataaa acgatggaag
    12721 gggtcaacca catcctagct gaaaatggtg gagaacttga tcttcgtgat gaagacttcc
    12781 aagcacttga agcagctaaa gatttgaacg atttccttgg tatcaactac tacatgagtg
    12841 attggatgca agcctttgat ggtgagactg aaatcattca caatggtaag ggtgaaaaag
    12901 gaagttctaa gtaccagatt aaaggtgttg gtcgccgaat cgcaccagat tatgtgccta
    12961 gaacggattg ggactggatt atttatcctg aaggcttgta tgaccaaatc atgcgagtga
    13021 aaaatgatta tccaaactac aagaagattt acatcactga aaacggtctt ggatacaaag
    13081 atgagtttgt agataatacc gtttatgatg atggtcgtat tgattacgtg aagaaacact
    13141 tggaagtttt atcagacgca attgcggatg gtgcaaatgt taaaggttac ttcatctggt
    13201 cacttatgga tgtcttctca tggtcaaacg gatacgaaaa acgttatgga ctattctatg
    13261 tagactttga cacgcaagaa cgctatccta agaaatcagc tcattggtat aagaaagtag
    13321 cagaaactca agtgatagag taatattact tgaaaatgta aaaaattgtt atccgatact
    13381 ttagaaaaag tacaacactt atttagacca ggctttgaaa aggtatatat tgttagtttc
    13441 gaagattgtc ctatgattcc tgagcttgaa gctaccccgt tgctaaaatg tggtaaatgg
    13501 tatgtttcaa ctggaaaaga gtggatttgc cattctgatt tagagttatc agcatttgaa
    13561 tgggaatttt tacaatcact cgatgtagaa atacgtgaga ctattcactt cgaggtaaat
    13621 tatttgcctt tccaataata aaaagaaagg aattagttgt gactatcgaa ttaaaaaacg
    13681 agtatcttac tgttcagttt aaaacacttg gtggtcaact gacttcgatt aaagacaagg
    13741 atgggataga gtatctctgg caagctgatc caaattattg gaatggacag gcgcctattc
    13801 tatttcctat ctgtggtagt ttacgaaatg attgggctat ttatagacct caagaaagac
    13861 ccttttttac aggtcttatt cgtagacatg gttttgttcg aaaagaagaa tttattctgg
    13921 aagaagtcaa tgataatagc gtgaccttta gtattaaacc aaatgctgag atgcttgata
    13981 attacctata tcagtttgaa ctgagagttg tttatacctt gaatggtaaa tcaattagaa
    14041 ccgaatttca ggtaacgaac ttggaaatgg aaaaaacaat gccttacttt attggagccc
    14101 atccagcatt caatagtcct ttagtagaag gtgaaaacta tgaggattat tatattgaat
    14161 ttagtgaggt tgagtcttgc tcgataccaa agagttttcc tgagacagga ttattagatt
    14221 tgcaagatcg gacacctttt ttggaaaatc aaaaaattct cgatttggac tactcacttt
    14281 tttcccatga tgccattaca cttgaccagc tcaagtctcg tagcgtaacg cttcggtcaa
    14341 ggaagtctgg taaaggtctt cgagtagatt ttgatgattt tccgaacttg attctgtggt
    14401 ctacaactaa taaaagtcca ttcattgctt tggaaccttg gagtggtctc tccacttcac
    14461 ttgaagaagg taatttctta gaagataaaa gacaggtaac gaaaatttca cctcaagaaa
    14521 cttctagaaa aatctatgat attactatcc ttaattaaaa tttgttatta tttgagtctt
    14581 caataagatt tgaatactct taccttgtga tgatagattg gtcttctccg tgccagtctt
    14641 acatcgcaag gtttttaagt tttttgtggg tctatgtcaa ctgtagtggg tgacgaaaag
    14701 ctaatatcta aagacagtct ggtagtaaaa aataagatta tttactactt cttgatttaa
    14761 aataatagtg gagccattaa tttgtggcaa atttaattta atattttgcc tcaaattagc
    14821 cacacttttt atagaactaa gaggaaaaat gaaattttct tcctattaat atagttgttt
    14881 gaaatcttaa atatagcgt
//
LOCUS       DACEEZ010000069        14815 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20313_2017
            SAMN12639966-rid9879223.denovo.069, whole genome shotgun sequence.
ACCESSION   DACEEZ010000069 DACEEZ010000000
VERSION     DACEEZ010000069.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639966
            Sequence Read Archive: SRR10023158
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 14815)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14815)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741683.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741683.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:44:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,016
            CDSs (total)                      :: 2,962
            Genes (coding)                    :: 2,889
            CDSs (with protein)               :: 2,889
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 36 of 73
            Pseudo Genes (incomplete)         :: 29 of 73
            Pseudo Genes (internal stop)      :: 18 of 73
            Pseudo Genes (multiple problems)  :: 8 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14815
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639966-rid9879223.denovo.069"
                     /isolate="E20313_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(296..1024)
                     /locus_tag="IWO89_002118"
     CDS             complement(296..1024)
                     /locus_tag="IWO89_002118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAQ0456840.1"
                     /translation="MLRIAIVDDDCTIVEGIRHYLLKKVPDIIIDHCYTDGDEAFKNL
                     QNQRFDLLITDITMGKISGLELIESLQQTSNRYFTSIVISAHQNFQYAKKGIDLSIKS
                     YITKPIDFPYLVKLVQEEKMRKSAANQNSLTDDSFNESIQQVIDYVQNNYSEKLTLKD
                     VATQFHYHPAYLGRAFKKETGYSFHDYLLDFRIKRASALLKDTFLSIDEISQKVGIDH
                     VSYFSVLFKKTTGHSPSNYRKKLL"
     gene            complement(1002..2576)
                     /locus_tag="IWO89_002119"
     CDS             complement(1002..2576)
                     /locus_tag="IWO89_002119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HAQ0456841.1"
                     /translation="MKNDLSKVIMRFTYILIFFITLLACFTLFFSYINVIEKEKNQNL
                     QNIQQITHYMETYNRILSGIVTEIYQNYDFQENLNDYYLSSPAEYMEDILSKDRSPTY
                     LVDNLKKLVQDDEVKNISISLDYEKYLSAYAPTKNEKISFIRSLSFISEDMPDATLIL
                     DVSINALKKSLFPTHYNYSLSNGIDSVNLSEDISPSKWKKTQQIAIGNHTLQVYENPN
                     YSSQTLLGLAIMIFLLMSLFILAIHQLLNRSFRNYIQQYQEIITSLNHSSTGDYKSIN
                     IENKVGDLKEIAMHINQFYLDNATYQKTLYENNWLKQEAKYAALQYQIEPHFLFNNLE
                     FIRMTAILNDDEEISDSIYLLSKLYRNNTYQPDIIDLKEELINIDLSLQLHQRRYRHN
                     FDYQLIDHIDQLMLPKLSLQPFVENYIKHGFDSSKKENRLTVYATETTNHYVLKIVEN
                     GKGMDQDKLEHIQQKLNDDIPYSENIGISNSFRRIKHLYPGDTRFVIESQLHVGTTIT
                     IKIQKGGNMHVENCNC"
     gene            2805..3743
                     /locus_tag="IWO89_002120"
     CDS             2805..3743
                     /locus_tag="IWO89_002120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAQ0456842.1"
                     /translation="MKLKKGLLGNLKKNRALFIMLLPGALWLFIFCYIPMFGVVIAFK
                     DFRISSRGFFTSLFNSPWVGWENFKYLFASQDAWIITRNTVVYNVVGILLSTFCTVSL
                     AIILSQMANKRLLKKMQTFMIFPNFLSWVVISYFVYAFLSTDKGLINILLSNFGVEPI
                     NWYSEPKYWPFIIVFTSLWKGVGYGSIIYYASIMGIDTSYYEAALVDGASKWQQIRNV
                     VLPHLRSIIVIMLILSVGSIFRADFGLFYQLPRNSGALYDVTNVLDTYVYRGLMNSGD
                     IGMSTAAGLYQSSVGFVLILITNKIVNKLDPSSALF"
     gene            3755..4666
                     /locus_tag="IWO89_002121"
     CDS             3755..4666
                     /locus_tag="IWO89_002121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HAQ0456843.1"
                     /translation="MISKLKRQMYFSLPVERFFTILLILFTLSCIFPFIFVIVISLTD
                     EKALQLNGYELLPAQWSTDAYQYLIQDGGQLLRSLGVTIMITVIGTLITVFMTGTYAY
                     VLSRASFPYRKFFTFYLFFTMLFAGGMVPSYLVMTKMLGLKNTIWALILPLAFSPYNV
                     IILRTFFKKSIPESIIESAKMDGCSEFRVFFQIVLPLAIPGVATIGLFSSLGYWNDWF
                     NALLYIDTNKLIPLQYLLMKIQNSMEFLANNNDITLAQQQAIQNSLPQESTRMAMVVV
                     ATLPIAIVYPFFQKYFVQGLTIGGVKE"
     gene            4690..6162
                     /locus_tag="IWO89_002122"
     CDS             4690..6162
                     /locus_tag="IWO89_002122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HAQ0456844.1"
                     /translation="MKKNMQKYLIGGASVLTLGLLGACGSSNGEANNETGTTKVTWYQ
                     VGEEPKDFDEVMTKVNEKLKEEINVELDMRLIPDGDYQQKLGVMINSGEEYDICFVNG
                     TDYVNYGNKGAFISLNDHLDKELKDYAAELNEGFIEGGAINGETYGLPVNNDIATTEY
                     WTFNKDLVDQYQLDVENVEGVETLAPVLEKFAEKNTDTNITPIGMTRTSVCIPYDNII
                     GGGVPLAVAFEGDTQTVVNTYETPEMMTYLKAMHEFYKKGYILKDAATVTTDPFRIDS
                     KNWFLGRNSSGYQPLAQKKLEALAGHGLILKPAGKAFKTTGFARVAMHAVSASSKHPE
                     AALKLLNYVNTNQEVADLMVYGIEGKHYDRNEDGSITLTNSDDYYQGAWALVNTYLVS
                     KTSPITEESKKLEEESKKFQNEAPMSPILGFSFDASDFRTEIASVQNIVSQYEVLLSS
                     GTVDPEQYVPELLEKLEKAGLDKIQASAQEQYDEWRKSNK"
     gene            6213..6824
                     /locus_tag="IWO89_002123"
     CDS             6213..6824
                     /locus_tag="IWO89_002123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF624 domain-containing protein"
                     /protein_id="HAQ0456845.1"
                     /translation="MNENKWTKGFNWVFQLLLLNFTYFILICLGFGILGFFPATIALI
                     AGVVNRKKYGLQESVKFMSKIYKKNFVRYNLYCWSATLLLAGNITTIWLCWQQSAPLF
                     YLLSTLLSVITLFLVFAMLNWVVIKSLGLWMMGDRLIDYLLFTATMLPTVVMQGLVLG
                     IYFRILMILPVIFLFGGTAVTVILISKLTERALAKLPCQIQSH"
     gene            6854..8767
                     /locus_tag="IWO89_002124"
     CDS             6854..8767
                     /locus_tag="IWO89_002124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-N-acetylhexosaminidase"
                     /protein_id="HAQ0456846.1"
                     /translation="MKLTDKQVANIAFIQQLEIPLTQKDEEQIDLIFTDTKANVRQIK
                     ITKNEQQRICLSYSHEDLLYRGLFICLPRFLAQGGEMPYIEETSIIETSYSLDASRNG
                     VMNKETLKKFISYLALMGYNTLYIYTEDTYEVEDLPYFGYLRGAYTKEEVNEIVEFAS
                     GFGIEVVPSIQTLAHLTQFLKWYAQQELMDDQNTLLIGEEKVYAAIEKMIIACKEMYQ
                     TERIHLGMDEAYQAGLGRYLSLYGYCDRTTLMIQHMDRVLSIVDKYKMKPMIWSDFIY
                     KLLDETGKDRYYNPDFKLKALAAKGLPAGLTYVHWDYGSDEVSSYEKVIDNHLKFCDI
                     DHYVLASGAHIWGRLAPNHGKTEAIVSAAVKASKNKGVRSVMLTTWGDEGQETEHWHS
                     LIGALKLADYVYSEEEWNDFEEIYDRLLGTGSYQLMSELRYFDEVDTITNNNLQMASV
                     SKLLLWQDPLVGIYDFHVAAHNKIATRTIGTYYHELAQKLDMNKNWNPKQPILTTIRQ
                     RYILLAEILAQKAELGIYLQHCCLVEDLAGLMELANRCEALANRVNELASIHEIIWYQ
                     TYKTYGWEVLEQRYAALAHRLKTVARRIKNHLQGQDALEELLEERLPFCQYQVPLEIS
                     GFNYRQSSVSGYN"
     gene            8895..11876
                     /locus_tag="IWO89_002125"
     CDS             8895..11876
                     /locus_tag="IWO89_002125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 42"
                     /protein_id="HAQ0456847.1"
                     /translation="MLSDYYQNLSVISVNTEPLRNYYIPYASKEEAKQELEREKTSQF
                     IDLNGTWDFQYYPSVQQLPENFFQEAFTESYDRISVPGVWQTEGFDLPQYINVSYPIP
                     FDPPYVPNDTPCGVYRRQFQIEQTDARFYLNLEGADSNCFVWINRNFVGYDQVSHSTS
                     EFDVTKYLQVGVNEIQILVMKWCDGTYFEDQDKWRMSGLFRPVYLLQRPTMHLTSYQL
                     RTLTDPKKGSGTLMVSFQRFTPIEVSLPISYELFHPDGRLIAKGECYSEQQINLDVED
                     CLFWTAETPKLYTLLLTCNGEYFKEKVGFRALTIKNRKLLLNGTPIKLYGVNYHDVNQ
                     QGTPVMTREQLRADLLLMKKLNVNTVRTAHYPKSPDFYELADEIGLYVINEADLECHG
                     VVNLYDTEANYQLIANDPTFEKPIMDRVIRSILPYQNRSSIIMWSMGNESGYGVNFEK
                     AQALARKLDDSRLIHNERSIEPVIDQVNDFSNLDVISQMYPPLEDVHRHCQRSDTEKP
                     FFMCEYSHAMGNGPGDLKTYFDLIQQYDSFLGGCVWEWTDHAINRGTLETPSYLYGGD
                     FGEKYHDGNFCVDGLLTPDRQLTVGALEYRNIHSPIQLIAVDEELQEVTLSNITAFTA
                     GSAFNLSLEWYIDGKKTVYEVMNPFILEPGKSCSLPITVPTTIHGAHTVAVSLSVYKN
                     DELYGQQQLFLKKEKMRLPRTAHHNDAPQISVKGTELIIKGESFIYIYQLAKAAFTSV
                     KINQKELLERPTQFSIWRAPTDNDRNLVNKWKAAGYAEMSLEIRKKDWKFVDDHLLIE
                     TSYRGVIPGLQPIFTMHIQLKINGAGVINQKVSVEKNPIFPDFPRFGMDWPLNDTFSK
                     VSYCGYGPYESYPDKHYAAMFGIYEMEIEEFDQPYIKPQEYGNRWQCDWVSIKNHQGS
                     TMTVYGNSNFSALPYSQAQLTEKSHHYELIKENRTWFNLSYKQNGIGSHSCGPYLEDR
                     FRFMEDTFDWNIVITFKGENR"
     gene            11873..13534
                     /locus_tag="IWO89_002126"
     CDS             11873..13534
                     /locus_tag="IWO89_002126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hyaluronate lyase"
                     /protein_id="HAQ0456848.1"
                     /translation="MKETKINQVKKKLSRYQEVVYRNHCLKWSKNKLNVRFNGLFYRF
                     EQLEEVWKPVTNFQSCPVQLINDPKETYDLCITHLYNQHLKNDGFYLKVTNDNQLTIE
                     SANLRGFQYAMEALLESFFSVGDQLLLPELILKHEPSVKIRGIIEGFYGIPWTHEMRL
                     SLFSYMKDNQLNTFMYAPKDDELLRKKWRELYDAQELDKFKELLSAAEASNIDFWYLI
                     SPGNDIDITCEEDIQVLLKKLEQLIELGVFQFGLLMDDIDYQLKGAAKRRFREPAFAH
                     AYLVNRVEEYLSTRLSNHELVICPTEYDNRHGSRYLATLSQEIPEQLPFFWTGPSTLA
                     ASISTEELQEMASVYQRPMLIWDNIPVNDYLEDKELLFMSPYENRTPNLSKERYQVTG
                     VVSNPMAQLEASKFTINSMANYLWNCERFDPLETWTSVVEKVVGSKLQPAYLTLTNAF
                     PNHYTSSLLSDEELLLAKDPEWVTKEMAALVQAVKWIQQEQHTSRLRTELEPWLQAIT
                     ESWTFWQEWQLKKDPKEAEEWLAKKKTHRIGRDLVTAHLEQIIKS"
     gene            13574..14506
                     /locus_tag="IWO89_002127"
     CDS             13574..14506
                     /locus_tag="IWO89_002127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016172406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="haloacid dehalogenase-like hydrolase"
                     /protein_id="HAQ0456849.1"
                     /translation="MVKRLISANYSDVKKMTSKELKQSIKASEGRVIVCETVANTLPQ
                     SGEITNAEVASAFGADLILLNGFDCYRPAVFGLPNVTIEKVFDLQQTIENPIPKLKEL
                     VGRPVGINLEPIAPKTNMMSEKVEISQGRASSVETIQKAEELGVDFICFTGNPGTGVT
                     NLEITKSVKMAKKYFSGLIIAGKMHSAGSEEPVVNKEAIISYAEAGADILLVPSVGSI
                     PGFLEQDLIEAVRLAKKYDLLTMSAIGTSQESARPETLRQLAIMNKSCGVDLQHIGDA
                     GYGGLAPAENIFETSVAIRGMRHTINCVSRSINR"
BASE COUNT         4815 a         2333 c         2978 g         4689 t
ORIGIN      
        1 atttaaataa ctacatccat ttattctatc ttctaaaata gcctttttca tttcagttaa
       61 atagttcagc gccatttttg cactcgactt gcatagtagt tttttccata ttcctcaacc
      121 tcagcggggg ttgaagacat aaggaaaatc ctatgcgtta tgctcaactg gatctgatta
      181 aatattctaa tgtatttttc tctaccatca ttaaatggtt aatagccaaa aatcaccact
      241 tttcaatggc tccttgttgt ataagtataa ctagaagatg aacacgtctg agaaattata
      301 gaagcttttt acggtaatta ctggggctgt gtccagtagt ttttttgaag agaacagaaa
      361 aatagctcac gtgatctatt cctacttttt gactaatttc atcaatagat aagaatgtgt
      421 ctttcagtag agcacttgct cttttgattc gaaagtctag caagtaatca tgaaaggaat
      481 aacctgtttc ttttttgaag gcccgtccta aataagcggg atggtaatgg aattgtgtag
      541 ctacatcttt gagcgtaagc ttttcactat aattgttttg aacgtaatca atcacttgtt
      601 gaattgactc attaaaagaa tcatcagtta gagagttttg attagctgct gatttacgca
      661 ttttttcttc ttgtactaac ttaactaaat aaggaaaatc aatgggtttc gttatgtaac
      721 ttttaataga taaatcaatc cccttttttg catactgaaa attttggtgg gcactaatga
      781 caatactagt aaagtagcga ttggatgttt gttgcaaaga ttcgattaat tcgagtccag
      841 aaattttccc catcgtaata tctgtgataa gtaaatcaaa gcgctggttt tgcaggtttt
      901 taaatgcttc gtctccgtct gtgtagcagt gatcgataat gatgtcgggg actttcttca
      961 aaagataatg ccttatgcct tctacaattg tacaatcatc gtcaacaatt gcaattctca
     1021 acatgcatat tccccccttt ttgtattttg atagtaatcg ttgttccgac gtgtagttgg
     1081 ctttctataa caaaacgtgt atctccagga tagagatgct ttatacgtcg aaaagaattt
     1141 gagatgccga tattctcact ataaggaata tcatcattaa gtttttgttg aatatgctcc
     1201 agcttgtctt ggtccattcc tttcccattt tcaacgattt ttaacacata atggttggtt
     1261 gtttcagttg catagacagt caaacgattt tccttttttg atgaatcgaa gccgtgttta
     1321 atataatttt caacaaatgg ctgtaaagaa agttttggta acattaattg atcaatatga
     1381 tcaatcagct gatagtcaaa gttatgtcga taacgacgtt gatgtagttg caaggagagg
     1441 tcgatattaa ttaattcctc tttcaaatca attatgtcgg gctgatatgt attattgcga
     1501 tacaacttgc tcaataaata tatactgtcg ctgatttctt cgtcatcatt caatatggca
     1561 gtcattcgaa taaattccaa attattaaat agaaaatgag gctcgatttg atattgaagt
     1621 gctgcatact ttgcttcttg cttcaaccaa ttattttcat aaagtgtttt ttgatatgta
     1681 gcattatcta aataaaactg attgatatgc atagcgattt ctttcaaatc accgactttg
     1741 ttttcgatat tgattgactt gtagtctcct gtagatgaat gatttaagga agtaatgatt
     1801 tcctgatact gctggatata gttacgaaaa cttctattta ataattgatg aatagcaaga
     1861 ataaacaaac tcattaataa gaaaatcatg attgccagac ctaataaggt ttgtgacgaa
     1921 taatttggat tttcatatac ttgtaatgtg tggttaccga tagctatctg ttgcgttttt
     1981 ttccatttag aaggcgagat gtcttcagat aaattgacgg aatcaatacc attagacaaa
     2041 gaataattgt aatgtgttgg aaagagactc ttttttagcg cgttgatcga aacgtctaga
     2101 atcaatgtag catcgggcat gtcctcacta atgaaactta atgaacgaat aaaagaaatt
     2161 ttttcgtttt ttgtaggtgc gtaggcactt aagtattttt cgtaatctaa tgagatagat
     2221 atatttttaa cttcatcatc ttgcacaagt ttttttaagt tatctactaa ataggtaggg
     2281 gagcgatcct ttgacaaaat atcttccatg tattcagcag gtgatgagag gtagtagtca
     2341 ttaaggtttt cttggaaatc ataattttga tagatctctg tcacgatacc ggagagaata
     2401 cgattatatg tttccatata gtgggtgatt tgctgaatgt tttgcaggtt ttgatttttt
     2461 tctttttcaa tcacattgat ataggaaaag aacaatgtga aacaggcaag taaagtaata
     2521 aaaaaaatca atatgtatgt aaaacgcata ataacttttg ataaatcgtt tttcattcaa
     2581 agtcctcatt ttgataatct ctaattttat gaatcattat agcacatatt acaagtgatg
     2641 taaacgcttt gcaaacaggg ctacttatga ttgtgaatac agtttccgtt taaaaactat
     2701 tctattgtag aggtttaata aactaaaaat aatgtataaa aaattaatgt aagcgattaa
     2761 ccaagtgggt ataatcaagg aaacgctgag agaggagaga caaaatgaag ctaaagaaag
     2821 ggttattagg aaatctaaaa aaaaaccgag cattgtttat tatgctttta ccaggtgcgt
     2881 tatggttatt tatattttgc tatataccaa tgtttggagt tgtaatcgct tttaaagatt
     2941 tcagaatttc atcaagggga ttctttacaa gtttgttcaa cagtccttgg gttggctggg
     3001 aaaatttcaa atatctcttt gcttcgcaag atgcttggat cattactcgc aatacggttg
     3061 tttataacgt tgtaggtatt ttgctaagta ctttttgtac agtgtcatta gcaatcattc
     3121 tgagccaaat ggcgaacaaa cggttattga aaaagatgca gacatttatg atttttccta
     3181 attttttatc atgggtagtc atcagttact ttgtttacgc atttttgagt acggataaag
     3241 gattaattaa tattttacta agcaattttg gtgtggaacc cataaattgg tatagtgaac
     3301 cgaagtactg gccttttatt attgttttta cgagtttatg gaaaggcgta ggctatggat
     3361 ccatcattta ttacgcgtca attatgggga tcgatactag ttattatgaa gctgctttag
     3421 ttgatggtgc cagtaaatgg caacagataa ggaatgtagt cttgccacat ttaagatcga
     3481 ttattgtcat tatgttgatt ttgtctgttg gtagtatttt tcgagctgat tttggattgt
     3541 tttatcaatt gccaagaaat tcgggggctc tttatgatgt gacgaatgta ttagatactt
     3601 atgtctaccg tggattaatg aatagcggag atatcggaat gtctacggca gcgggattat
     3661 atcagtcatc agttggcttt gtcttgattt tgattactaa taagatagtc aacaagttag
     3721 atcctagttc agcactattt taggaggaat caaaatgata tcaaaattaa aacggcagat
     3781 gtacttttcg ctgccagtcg aacggttttt cacaatatta ctaatcttat ttactcttag
     3841 ctgtattttt ccctttattt tcgtaatcgt catttcatta acagatgaaa aagcattaca
     3901 actcaacggt tacgaattat tgccggctca atggagcact gatgcatatc aatacttaat
     3961 acaagatggc ggacagttac ttcggtcatt aggcgttaca attatgatta cagtgattgg
     4021 aacgttgatt actgttttta tgacgggaac ctatgcttat gttttatcaa gagcctcttt
     4081 cccttaccgg aagttcttta ctttttatct tttttttacc atgttatttg cgggagggat
     4141 ggttccttct tacttagtga tgacgaagat gcttggctta aaaaatacaa tttgggcttt
     4201 gattctacct cttgctttta gcccatacaa tgtcattatt ttaaggacat tctttaagaa
     4261 gtcgattcca gaatcgatta tcgaatccgc taaaatggat ggctgtagtg agtttcgagt
     4321 ttttttccaa atcgttttgc ctttagcgat cccaggggtt gcaacaatag gtttattttc
     4381 aagcttaggt tattggaatg actggttcaa cgcattgctg tatattgaca ccaacaaatt
     4441 gattccattg caatatttgt taatgaagat tcaaaattca atggaattct tagcaaataa
     4501 taatgacatt acattagcgc agcaacaagc aattcaaaat agccttcctc aagaatcaac
     4561 acgaatggca atggtcgtag tagctacttt accgattgca attgtttatc cgttcttcca
     4621 aaaatacttt gttcaaggat tgacgatcgg tggagtgaaa gaataaaaaa gaataggaga
     4681 tgttaaaaga tgaaaaagaa catgcagaaa tatttgattg gtggtgcttc tgtactaacc
     4741 cttggcctat taggtgcttg tggtagcagt aatggcgaag caaataatga aacaggaacc
     4801 acaaaagtta cttggtatca ggttggagaa gaacccaaag attttgatga ggtcatgaca
     4861 aaagtcaacg aaaaattaaa ggaagaaatc aatgtagagt tggatatgcg gctgattccg
     4921 gacggggatt atcagcaaaa gcttggtgtg atgatcaatt caggagaaga atatgatatt
     4981 tgtttcgtca atggcacaga ttatgtgaat tatgggaaca aaggtgcttt cattagtctc
     5041 aacgatcatt tggataaaga gttgaaagat tatgcagctg aattgaatga aggatttatt
     5101 gaaggtgggg caattaatgg cgaaacttat ggattgccag tcaataacga tattgccaca
     5161 acggaatatt ggacctttaa taaagatttg gtagatcaat accaattgga tgtagagaat
     5221 gttgaaggtg tagaaacatt ggctccagta ttagaaaagt ttgcagaaaa aaatactgat
     5281 acgaatatta cgccaatcgg tatgacccgt accagtgtct gtattcctta tgacaatatt
     5341 atcggtggtg gtgtaccgct agcggttgca tttgaaggcg atacacaaac agttgttaat
     5401 acttatgaga ctccagaaat gatgacttat ttgaaggcta tgcatgagtt ttataaaaaa
     5461 ggatatattt tgaaagatgc agccactgtt acgacagatc catttcgaat tgatagtaaa
     5521 aactggtttt taggtcgtaa ctcaagtggt tatcaaccat tagcacaaaa aaaattagaa
     5581 gctctagcgg gacatggatt aattttaaaa ccagctggaa aagcttttaa aacgactgga
     5641 tttgcaagag tggcaatgca tgcggtatct gcttcaagta aacatccgga agcagccttg
     5701 aaattgttga attatgtgaa taccaatcaa gaagtagcgg atttaatggt ttatggaatt
     5761 gaaggaaaac actatgatcg caatgaagat ggttcgatta ctttaacaaa ctctgatgat
     5821 tattatcaag gcgcttgggc attggtaaat acttatctag tttcaaaaac ttcaccgatt
     5881 acagaagaaa gtaaaaaact agaagaagaa agtaaaaagt ttcaaaatga agcaccgatg
     5941 tctcctattt taggtttctc ttttgacgct tctgattttc gaacagaaat tgcaagtgta
     6001 caaaatatcg tgtcacaata tgaagtactt ttaagctcgg gaacagttga ccctgaacaa
     6061 tatgtaccag agttgttaga aaaactagaa aaagctggat tggataaaat tcaggcatct
     6121 gctcaagaac aatacgatga atggcgaaaa tctaacaaat aattttttac taaatgaaaa
     6181 gaatagacta agcagttagt ggggggattc aaatgaacga aaacaaatgg accaaaggat
     6241 ttaattgggt ttttcaattg ttgttactaa actttactta ttttatacta atttgtttag
     6301 gatttggaat tttaggtttt ttccctgcta cgatagcttt gattgctggc gttgtgaatc
     6361 gaaaaaaata tggcttgcaa gagtctgtga agttcatgag taaaatctat aagaaaaatt
     6421 ttgttagata taatctatat tgctggtcag caactttgtt gttggccggc aatattacta
     6481 cgatctggct ttgttggcag caaagcgcac cattgtttta tttattaagt acattattat
     6541 cggtaattac actgttccta gtctttgcaa tgttgaattg ggtcgtgatc aaaagtcttg
     6601 ggttgtggat gatgggagac cgactgattg actatttgct tttcacagca acgatgttgc
     6661 caacggtagt catgcaaggc ttggtgcttg gaatttattt ccgaatattg atgattttgc
     6721 ctgtgatttt tctgtttggc ggaacagctg taacggtaat attgattagt aaattaactg
     6781 aaagagcgtt agcaaagttg ccttgtcaga tacaatcgca ctaatttttt cacaaaaagg
     6841 agtatttctt tctatgaagt taactgataa acaagtagct aatatagcct ttatccagca
     6901 acttgagatt ccattgacac aaaaagatga agaacaaata gacttgattt ttactgacac
     6961 aaaggctaat gtgcgacaaa tcaagattac taaaaatgag cagcaacgaa tttgcttgtc
     7021 ttattcacat gaagacttgc tgtatcgagg attgtttatc tgccttccac gttttttagc
     7081 tcaaggagga gagatgccat acattgaaga aacctcgatc attgagacca gttattcatt
     7141 agatgcttct cgtaatggcg tgatgaataa agagactcta aaaaaattca tttcttactt
     7201 agcgttgatg gggtacaaca cattgtatat ttacacagaa gatacctatg aagtagaaga
     7261 ccttccatat tttggttacc ttcgcggagc gtatacaaaa gaagaagtga atgaaattgt
     7321 tgaatttgct tctggttttg gcatagaagt agttccatcg atccagaccc ttgctcactt
     7381 gacccaattt ttgaagtggt atgctcaaca agaactgatg gatgatcaaa atactctact
     7441 gatcggtgaa gaaaaggttt atgcggcaat cgaaaagatg attattgcct gcaaagaaat
     7501 gtatcagaca gagcgtattc atttgggaat ggatgaagcc tatcaagcag gtctgggacg
     7561 atatttaagc ctgtatgggt attgtgatcg gacaacttta atgattcaac atatggaccg
     7621 tgtgttatca attgttgata aatataagat gaaaccaatg atttggagtg atttcattta
     7681 taagctatta gatgaaaccg gtaaagatag gtattacaat cctgatttta aactcaaagc
     7741 attagcagct aaaggattac cagcaggatt aacctatgtt cattgggact atggttcaga
     7801 tgaagtttcc tcctatgaga aagtaattga taaccatttg aaattttgtg acattgatca
     7861 ttacgtttta gctagtggtg cccatatctg ggggagacta gccccgaatc atgggaaaac
     7921 agaagcaatt gtttctgcag ctgtaaaagc ttctaaaaat aaaggtgtac gttctgtgat
     7981 gttaactact tggggagatg aagggcagga aacagagcat tggcattcct taattggggc
     8041 attaaaatta gcagattatg tctattctga agaagagtgg aatgattttg aggaaatcta
     8101 cgatcgttta ttaggaacag gtagttatca attaatgtcc gaattacgat atttcgatga
     8161 agtggatacc attacaaata acaacttaca aatggcttcc gtgagtaaac tgctactttg
     8221 gcaagatccg ttagttggta tatatgactt tcatgtagcc gctcataaca aaattgccac
     8281 tagaacaatt ggtacttatt atcatgagtt agctcagaaa ttagatatga ataaaaattg
     8341 gaatccaaaa caaccaattt taacaactat tcgtcaacga tatatacttt tagctgaaat
     8401 tttagcacaa aaggcagaac taggcatcta tttacaacac tgctgtctgg tagaagacct
     8461 tgcaggatta atggaattag cgaatcggtg tgaagcacta gccaatcgag taaatgaatt
     8521 ggcatctatt catgaaataa tttggtatca aacatacaaa acctatggtt gggaagtatt
     8581 agaacaacga tatgctgcat tagctcatcg tctgaaaacc gttgcgcgtc gtattaaaaa
     8641 tcatcttcag gggcaagatg ccctagagga gcttttggaa gaacgattac cgttttgtca
     8701 atatcaagtg ccgcttgaaa tttctggctt taattatcgg caaagctcgg tgtcaggcta
     8761 caattaacga aggaatcttc ccgttgaacg gaccacttgc tcatctaatg ggaaagtggt
     8821 tcgttttcta ttcataagta gcttaccact aactctttta ttaagaagag aaaaaaagta
     8881 ggaagtgaga aaatatgtta tcggactatt atcaaaatct gtcagttata agtgtcaata
     8941 cagaaccatt acgtaattat tatattcctt atgcaagtaa agaagaagca aaacaagagt
     9001 tagaaaggga aaaaacgagc cagtttatcg atctcaatgg tacatgggat tttcagtatt
     9061 atccttctgt ccaacagtta ccggaaaatt tttttcaaga ggcctttact gaaagctatg
     9121 atagaatttc tgttcctgga gtttggcaaa cagagggatt tgacttacct cagtatatca
     9181 atgtatctta tccaattcct tttgatccgc cttatgtacc taatgatacc ccttgtggtg
     9241 tatatcggcg tcagtttcaa attgagcaaa cagatgcacg cttttactta aatctagaag
     9301 gagcagatag taattgtttt gtttggataa atagaaactt tgtaggctat gaccaagtga
     9361 gtcatagtac atctgaattt gatgtgacca agtatttaca ggttggggtt aatgaaattc
     9421 aaattctagt gatgaagtgg tgtgatggta cctactttga agatcaggat aagtggcgaa
     9481 tgagcggtct atttcgtcca gtgtatcttc ttcaacgacc aactatgcat ctgacaagtt
     9541 atcaactacg gacgttgact gatcctaaaa aaggaagcgg aacattaatg gtttcttttc
     9601 aacgatttac tcctattgaa gtatcgttac ctatttctta cgaattattc catcctgatg
     9661 gacgtctcat agcgaaaggg gagtgttact cggaacaaca aataaatttg gatgttgaag
     9721 attgcctttt ttggacagca gaaacgccta aattatatac attactttta acatgtaatg
     9781 gtgaatattt taaagaaaaa gttggctttc gagcactgac gatcaagaat cgtaagttat
     9841 tgctgaacgg aacacctatt aaattatatg gagtcaacta tcatgacgtg aatcagcaag
     9901 gcacgccagt gatgacccga gagcagttac gtgcagattt gttattaatg aaaaaattaa
     9961 atgtgaatac tgtacgaacg gcacattacc ctaaatcacc agatttttat gaattggcgg
    10021 atgagattgg tctgtatgta atcaatgaag cagatttgga gtgtcatggt gtggttaatc
    10081 tatatgatac tgaagcgaat tatcaactaa ttgctaatga tccgacattc gaaaagccga
    10141 ttatggaccg agtgatccgt tctattcttc cttatcaaaa tcgaagctct atcattatgt
    10201 ggtctatggg gaatgaatct ggttatggag taaattttga aaaagctcaa gctttagcac
    10261 ggaaattgga tgattcacgg ttgattcata atgaacgctc gattgaacca gttattgacc
    10321 aagtgaatga ttttagtaac ttggatgtta ttagtcaaat gtatccccca ctggaagatg
    10381 ttcatcgtca ttgtcaaaga agtgatactg aaaaaccttt ttttatgtgt gaatatagcc
    10441 acgctatggg caacggtcct ggagatctta aaacgtactt cgatttgatt caacagtatg
    10501 attcctttct tggcggttgt gtctgggagt ggactgatca tgcaatcaat agaggaactt
    10561 tagaaacgcc atcttattta tacggtggtg attttggtga aaaatatcac gacggcaatt
    10621 tttgcgtaga tggtttattg accccagatc ggcaattaac agtaggtgca ttggagtacc
    10681 gtaatattca ttcgcctatt caactgattg ctgtagatga ggagctgcaa gaagtaacct
    10741 tgagcaatat aactgctttt acagccggtt cagcatttaa cctttctctt gaatggtata
    10801 tagatggaaa aaaaacagtt tatgaagtaa tgaatccttt catacttgag cctggcaaaa
    10861 gttgttcatt gcccatcaca gtacctacaa cgattcatgg tgctcatacg gttgctgttt
    10921 cgctatctgt ttataaaaat gatgagcttt atggacagca acagctcttt cttaaaaaag
    10981 aaaaaatgag attgccaaga actgctcatc ataatgatgc accacaaatt tcagtaaaag
    11041 gcacagagct gataatcaaa ggagaatcct ttatttatat atatcaatta gcaaaagctg
    11101 cgttcacttc tgtaaagatc aatcaaaagg aacttttgga acgtcctact caattttcaa
    11161 tttggcgtgc accaacggat aatgatcgta acttagtaaa caaatggaaa gcagctggat
    11221 atgctgaaat gtctttagaa atcagaaaaa aagactggaa attcgtagat gatcatctcc
    11281 tgatcgaaac gtcttatcga ggagttattc caggattaca gccaattttt acaatgcaca
    11341 tacagttgaa aataaatggt gcaggcgtaa tcaaccaaaa agtatcagtg gagaaaaatc
    11401 cgatttttcc agatttccct cgctttggga tggactggcc gttgaatgat actttttcga
    11461 aggtttctta ttgtggctat gggccgtatg aaagttatcc tgataagcac tatgctgcga
    11521 tgtttggtat ttatgagatg gagattgagg aatttgatca gccatatatc aaaccacaag
    11581 aatatggtaa ccgctggcaa tgtgattggg tatcaatcaa aaatcatcaa ggatctacca
    11641 tgacggttta tggaaactct aatttttctg ctctgcctta tagtcaagct caattaaccg
    11701 aaaagtcaca ccattatgaa ttaataaaag aaaatagaac ttggttcaat ttaagttata
    11761 aacaaaatgg tatcggctca cacagttgcg gtccttattt agaagatcgt ttccgtttta
    11821 tggaagatac ttttgactgg aatatagtga ttactttcaa aggagaaaat agatgaaaga
    11881 aacaaagatc aatcaagtca aaaaaaaatt atctcgttat caagaagttg tttatcgaaa
    11941 ccattgctta aaatggtcaa aaaataagct gaatgttcgt tttaatggat tattttatcg
    12001 tttcgaacag ttagaagaag tctggaaacc ggtaacgaat ttccaatcat gtcctgtaca
    12061 gctcataaat gatccaaaag aaacatacga tttatgtata acacaccttt ataaccagca
    12121 tttgaaaaat gacggatttt atttaaaggt tactaatgac aatcaactta cgattgaatc
    12181 agcaaacctt cgagggtttc aatatgctat ggaagcctta ttagagagtt ttttttcggt
    12241 aggagatcaa ttattattac cggaattgat tctgaaacat gaaccatcag tgaaaattcg
    12301 aggaatcatt gaagggtttt atggtatccc ctggacacat gaaatgcgcc tctctctttt
    12361 ttcatatatg aaagataacc aattgaatac ttttatgtat gcccctaaag atgatgaatt
    12421 attacgtaaa aaatggcgtg aactctacga tgcgcaagaa ttagacaaat ttaaggagct
    12481 tttatctgct gctgaggcta gcaatatcga tttttggtac ttaattagtc ctggaaatga
    12541 tattgatatt acgtgtgaag aagacattca agttcttctg aaaaagttag aacaactgat
    12601 tgagcttggc gtatttcaat ttggtctatt aatggatgat attgactatc aattaaaagg
    12661 tgctgctaaa cgccgttttc gagagccagc attcgctcat gcctatttag tgaatcgagt
    12721 agaggaatat cttagcactc ggttgtccaa tcatgaatta gtcatttgtc ctacagaata
    12781 tgataatcgt catggttctc gctatttagc aacgttaagt caagaaatac cagaacaatt
    12841 accgttcttt tggactggcc caagcacgtt agcggcgtca atttctacag aagagctgca
    12901 ggaaatggct tctgtctatc aacggccgat gctgatttgg gataatattc cggtaaatga
    12961 ttatttagag gataaagaac tattgttcat gagtccatat gagaatcgaa caccgaatct
    13021 ttctaaggag agataccagg ttacaggagt agtttcgaat ccaatggctc aattagaagc
    13081 ttcaaagttt acgatcaata gtatggctaa ttacttatgg aactgtgagc gttttgatcc
    13141 tttggaaact tggacgtcag tcgttgaaaa agtagttgga agtaaactac aaccagctta
    13201 tcttacctta accaatgctt ttccaaatca ttacacaagc agtctgcttt cagatgaaga
    13261 attactgcta gccaaagacc cagaatgggt cacaaaagaa atggctgctt tagtccaagc
    13321 agtaaaatgg atccagcaag aacaacatac atctagattg agaacagaat tggagccttg
    13381 gttacaagca atcacagaaa gttggacttt ctggcaagaa tggcaattaa aaaaagatcc
    13441 aaaagaagca gaagaatggt tggctaagaa aaaaacacac cgcataggac gtgatttagt
    13501 aactgcccat ttagagcaaa ttatcaaaag ttagatatct taaaaaataa aaagcaagaa
    13561 aaaggagaac aaaatggtca agagattaat tagtgcaaac tacagtgatg tcaagaaaat
    13621 gacgtcaaaa gagttgaagc aatccatcaa agcgagtgaa ggacgggtga ttgtttgtga
    13681 aacggttgct aatactcttc cacaatctgg agaaattacg aatgcagaag ttgcgtcagc
    13741 atttggtgca gatttgattc ttttaaatgg tttcgattgt tatcgtcctg cagtttttgg
    13801 actaccaaat gtgacgattg agaaggtttt tgatctacag caaacgattg aaaacccgat
    13861 tcctaaatta aaagaactag tcggtcgtcc tgtgggaatt aatttagaac ccattgcccc
    13921 taaaacaaat atgatgagtg agaaagtaga aatcagccaa ggaagagcaa gctctgttga
    13981 aacgatacaa aaagcagaag aactaggagt cgattttatt tgctttaccg gtaatccagg
    14041 cactggagta accaatttag aaattactaa atccgtaaaa atggcaaaaa agtatttttc
    14101 gggattgatt attgctggga aaatgcatag cgccggctca gaagagccag ttgtgaataa
    14161 agaggcgatt atttcttatg cggaagcagg cgcagatatt ttactagttc cttcagtagg
    14221 gtctatccca ggctttttag agcaagacct aattgaagcg gttcggttgg caaaaaaata
    14281 tgatttgtta acgatgtctg caatcggtac tagtcaggaa agtgcccgac cagaaacatt
    14341 gcggcaatta gccattatga ataaaagttg tggtgttgat ttacaacata ttggagatgc
    14401 tggatacggg ggattggcac cagcagaaaa tattttcgaa acctcggttg caattagagg
    14461 aatgcgacat accatcaact gcgttagccg ctcgatcaat cgctaacaaa attaaataaa
    14521 aaatggttaa attgattatt taagtgggaa catacttgag atatcagcta acttctgtca
    14581 ctttaaaaaa gggactgtga cagaagtgaa aatagacaaa aaatatccga acaagagact
    14641 aggatagcag aaaaactatc gttttgcaac gaggaccaac tggaccgtag gagcaattct
    14701 cttattgttc ggatatttcg cttgttcaag gacttatgtc tgtagtgccc ttttttggta
    14761 ggaaaaatgg tagggaacca tatcattttt aatgaaaatc aagcatttca aaagt
//
LOCUS       DACEEZ010000070        14690 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20313_2017
            SAMN12639966-rid9879223.denovo.070, whole genome shotgun sequence.
ACCESSION   DACEEZ010000070 DACEEZ010000000
VERSION     DACEEZ010000070.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639966
            Sequence Read Archive: SRR10023158
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 14690)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14690)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741683.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741683.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:44:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,016
            CDSs (total)                      :: 2,962
            Genes (coding)                    :: 2,889
            CDSs (with protein)               :: 2,889
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 36 of 73
            Pseudo Genes (incomplete)         :: 29 of 73
            Pseudo Genes (internal stop)      :: 18 of 73
            Pseudo Genes (multiple problems)  :: 8 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14690
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639966-rid9879223.denovo.070"
                     /isolate="E20313_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            <1..560
                     /locus_tag="IWO89_002128"
     CDS             <1..560
                     /locus_tag="IWO89_002128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000181735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-dependent helicase"
                     /protein_id="HAQ0456850.1"
                     /translation="KNEFGIEVKDYFIDLDLLQLINSVKRMYSGDDNGVDLYKFVTKH
                     IFVNLNIDEKNYPSIFKHYLFFINEVEDRIKNNSLSSSVSSFKKMFKSRDGITLTTAH
                     KVKGNEYDTVIAINLLNGKIPHWNEIFNVSDKGISSARKLLYVICSRAKNNLFLFSEV
                     GYFTSKGDLLYPTQLLDKIEFDYNI"
     gene            760..1074
                     /locus_tag="IWO89_002129"
     CDS             760..1074
                     /locus_tag="IWO89_002129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018705633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HAQ0456851.1"
                     /translation="MELKYVIPNMEKTFGQLEYAGEGNIEQRRINGRPTILSRSYNLY
                     STIQRADDIVVILPSEAGEKHFESDEKVKLINPHITAEGYKIGNRGFTNYIMHADDMV
                     KA"
     gene            1095..1472
                     /locus_tag="IWO89_002130"
     CDS             1095..1472
                     /locus_tag="IWO89_002130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001234189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HAQ0456852.1"
                     /translation="MRLAEGIVIEKDQTFGTLKFSALRREVRIQNEDGTVSEEIKERT
                     YDLKSKGQGRMIQVSIPASVPLKEFDYNAEVELINPVADTVATATFRGADVDWYVKAD
                     DLVLKGQTKPHQKYKKEASESEK"
     gene            1482..2255
                     /locus_tag="IWO89_002131"
     CDS             1482..2255
                     /locus_tag="IWO89_002131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002388713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0456853.1"
                     /translation="MTNTPLILKEIPKDEAISFIRQYHYSKILPRLCKYFLGIFSEEK
                     LLGVVELGWGTQPLQTIRKLFPDSSLQTTDYLEIGKMCFLPEMNQTNYFGSQALSALI
                     KWLKEHTDCHFLYTLADGIEGKCGYVYQASNFFYCGYFKTSVYRDKQSWEKIHPRSAR
                     LLLEENARFEQVEKKHWLSQAFCEYKGIEKINGRMFRYLYPLTKEAKKLLGHTLYRRH
                     YYPKEKNLRFEKRIAYQKYEAISQPTFDKQARIYNTQLF"
     gene            2277..3680
                     /locus_tag="IWO89_002132"
     CDS             2277..3680
                     /locus_tag="IWO89_002132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001130245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF87 domain-containing protein"
                     /protein_id="HAQ0456854.1"
                     /translation="MPLLHDRGERIKANDKHLVYHFVLISLLVVFLVVLLPFHWRALQ
                     SVNWQAVQLDTFFYQLHTPYFWSSILTALFVCGLISWLYYHYRIDSIKQLEHRQKLAR
                     MILENHWYEAEETQSEGFFKDLPSKKTKQRIRHFPKIYYRMKDGLIHIHVEITLGKYQ
                     DQLLHLENKLETGLYCELVEKTLKDSYVEYVLLYDTIGNRIGIEDVVAQDGRVKLMES
                     VYWEFDSLPHMLIAGGTGSGKSYFILTLIESLLHTNAKLYILDPKNADLADLGAVMNN
                     VYYRKEDMLACINQFYEDMIDRSETMKQHPNYKTGENYAYLGLSANFLIFDEYVAMMD
                     MLGRESTTVIHKLKQIVMLGRQAGFFLILACQRPDAKYFSDGIRDNFNFRVALGRMSE
                     MGFGMMFGSDTQKQFFLKPIKGRGYVDTGKSVISEFYTPLVPKRYDFLGEIGKVIQKK
                     QSEPVPHEVKGTGSDLL"
     gene            3862..5046
                     /locus_tag="IWO89_002133"
     CDS             3862..5046
                     /locus_tag="IWO89_002133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000398284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HAQ0456855.1"
                     /translation="MENEKIWIKELKEKRLAYGVSQNKLAVASHITRPYLSDIETGKA
                     VPTQQVKEDLLNALERFNPDNPLEMLFDYVRIRFPTNDVAQIIGEVLRLNMDYMLHED
                     FGYYSYPEHYRFGDIVVLVSHDVSKGVLLELKGKGCRQFENFLLAQHRSWYDFFQDCM
                     EHKGIFKRLDLAINDKTGILNIPDLAKKCKQEECISVFRSFKNYRSGELVHRDEKPYM
                     GNTLYIGSLKSEVYFCIYEKDYEQYVKNDVPLEDAEVKNRFEIRLKNDRATQAMKDLL
                     AHQQAEKTAFEIINRYIRFADKDDTKRRSDWKTNERWEWFIGKNRGALRLTTQPEPYS
                     FERTLNWLHHQVAPTLKIASILDVLNGTTIISTMIQEAKLTEKHEKLIEQQHLAMEDL
                     IT"
     gene            5043..5333
                     /locus_tag="IWO89_002134"
     CDS             5043..5333
                     /locus_tag="IWO89_002134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000055367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0456856.1"
                     /translation="MSLLTKELKKLGFQCGIEFQAYIQNTGKYTSLIIEGKRQAGDTI
                     YTYDFYKVRFYNNYTNRVTVYGEHLTPFQLLRRVKSYIYYREKYLKERRTIT"
     gene            5330..5551
                     /locus_tag="IWO89_002135"
     CDS             5330..5551
                     /locus_tag="IWO89_002135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002570387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0456857.1"
                     /translation="MNFGQNLYNWFLDNAQSLVLLAIVVIGLFLGFKREFSKLIGFLI
                     IALIAVGLVFNAAGVKDVLLNLFNRIIGA"
     gene            5593..6372
                     /locus_tag="IWO89_002136"
     CDS             5593..6372
                     /locus_tag="IWO89_002136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000678782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="abortive infection family protein"
                     /protein_id="HAQ0456858.1"
                     /translation="MKGFMNMFTNDQFKSLLIKTLERKSSSNYYEGGNEIVSKMKISE
                     VTLDETDDFTYYKGYKKGWNTLCTYLKIRVPFDDLDFFESHKDLITQTASSIYDKQGD
                     NVLVDTILVPLPENYEVINFSQLKISDVVSQAIEDAESFMSNGEYQRAFDRVHTAFHG
                     YLIEILKKYEITVPRDENLSKLYSRIQQLIEKEIQPTELADIVKTTIRSSNGMISSLN
                     EARNRHSLAHPNTNIIGKREAKLIIGISSTVTDYISGYLDK"
     gene            6439..6933
                     /locus_tag="IWO89_002137"
     CDS             6439..6933
                     /locus_tag="IWO89_002137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000870467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein ArdA"
                     /protein_id="HAQ0456859.1"
                     /translation="MKVYVANLGRYNEGELVGAWFTPPIDEEEMAERIGLNEDYEEYA
                     IHDFELPFDVDEYTPISEINRLCEAIQEIEGTPIYNELKEIQGMWFSSLEELLENKED
                     IHCYSDCDSMEDVARYYVEETGQLGEVPSNLQNYIDYQSLGRDMEIEGNYLVTSHGVF
                     EYCQ"
     gene            6989..7627
                     /locus_tag="IWO89_002138"
     CDS             6989..7627
                     /locus_tag="IWO89_002138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000248477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0456860.1"
                     /translation="MVNVFENLKLLKLDMKDKGWVIDSFYFRYKQQNYIVLVKLFEKE
                     EKVPEYALLKLEFLKENDFSDMLAVYANSVKLYTDTKTIREYFGIEYSSNLGDVLFQF
                     SQTLARFIPTEVSEKKNEDQKEAMCFSLSQSDSEDPRKKYCFSVRRNPLKGNGELGKR
                     SPFNDNKTRLYRPSLYERLGSDTNLSFRYSMNPDDEETDEGIIAKWTKNKIE"
     gene            7699..8094
                     /locus_tag="IWO89_002139"
     CDS             7699..8094
                     /locus_tag="IWO89_002139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_464632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HAQ0456861.1"
                     /translation="MKKIRSYTSIWSVEKVIYAINDLQLPFPITFTQMTWFIVSLFVV
                     ILFGSLPPLSLIDGALLKYLGIPIVLTGFMCQKTFDGKKPYGFLKSIITYALRPKVTY
                     AGKQIRLKKETMNESITIVRSEQLDGISD"
     gene            8078..10531
                     /locus_tag="IWO89_002140"
     CDS             8078..10531
                     /locus_tag="IWO89_002140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000331166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HAQ0456862.1"
                     /translation="MAYPIKYMEDNLVFNHDGEVFAYYELIPYNYSFLSSDEKIQVHD
                     NFCQLIAQNRDGKIHALQLATESSIRATQEQSKKEITGRLKEIAYEKIDEQTEALISM
                     IGDNQVDYRFFIGFKLYLNEQEVSVKNVGKEIQKAFAEFFHGVNHKLMGDFVSMPMAE
                     IERFSKMEQLLESKLSRRFQIRKLEKDDFGYIIEHLYGQTGVPFDDYDYHLPKRYEDK
                     KVLVKKYDILKPTRCLIEENQRYLRIEQEDTTTYVAYFTINSIVGELDFPNSEIFYYQ
                     QQQFTFPVDTSMNVEIVANKKALSTVRNKKKELKDLDSHAWESDNETSTNVVDALESV
                     DELESNLDQTKESMYKLSYVVRVSAKNLDELKQRCDEVKDFYDDLSIKLVRPFGDMLG
                     LHNEFIPSSKRYINDYIQYVTSDFLAGLGFGATQMIGENSGIYVGYNLDTGRNIYLQP
                     SLASQGIKGSVTNALASAFIGSLGGGKSFSNNMLVYYSVLFGGQATIVDPKAERGEWK
                     ETLPDIAHEINIVNLTSENDNKGLLDPYVILKLPKDSESLAIDILTFLTGISSRDSEK
                     FPVLRKAIRSVTQSKQRGLLLVIDELRKEDTPISNSIADHIESFVDYDFAHLLFSDGS
                     VTQSISLEKQLNIIQVADLVLPDSNSSFEEYTTMELLSVAMLIVISTFALDFIHSDRS
                     IFKIVDLDEAWSFLQVAQGKALSMRLVRAGRAMNAGVYFVTQNADDLLDEKMKNNIGL
                     KFAFRSTDLVEIKKTLEFFGVDKEDENNQKRLRELENGQCLVSDIYGRVGVMQFHPIF
                     EELLHAFDTRPPVRNEVEE"
     gene            10528..12555
                     /locus_tag="IWO89_002141"
     CDS             10528..12555
                     /locus_tag="IWO89_002141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000192391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0456863.1"
                     /translation="MTQKQKKFLHYSWITLLCVGVILLLLGTLGNAVQATGLVDETID
                     TSNEYSKYGLNQYQLDYYVDNSWGWLPWNWSDGIGQSVMYGLYAITNFIWTISLYLSN
                     ATGYLVKEAYALDFISQTSQAIGENMQTIAGITPNGFSSSGFYVGFLLLFILIVGIYV
                     AYTGLVKRETTKVIRAVLNFVVVFILSASFIAYAPDYIAKINDFSKDVSTASLNIGTK
                     IVLPNTDSQGKDSVDMIRDSLFSVQVKQPWLLLQYGSTDVEALGEERVESLLSTSPDT
                     NNGEDRENVVIEEIEDHDNTYLTLPKTISRLGTVFFLFVFNIGISIFVFLLTGIMIFS
                     QVLFIIFAMFLPISFLLSMLPSFDGMGRHAITKLFNVIMTRAGITLIITVAFSISTML
                     YSLSASSPFFMIMFLQIVTFAGIYFKLGDLMSLFTLQSNDSQNMGRQVFRRPRQMMNR
                     QTRRFQRSMSKIFRGKAGSTKQKTMTTKQNQTNKANHTRQNERTTPKKETALSNVKKQ
                     QGGIGQRLGEKTAHVLDTKNRLVNKRKQAKQQIQATPTHVKYALHQGKENVTHNVTDF
                     KQSMADTKAQRKQQRMNQQEQKRKNIAQKRLELDKAKEKRRKENPSSPVQNNQQHVRK
                     RQQTVTPVLSQNTKRENHQRPLKKEQKQKTPPKQFNQRKLNKRTDKRGQQT"
     gene            12552..13574
                     /locus_tag="IWO89_002142"
     CDS             12552..13574
                     /locus_tag="IWO89_002142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000768374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase P60"
                     /protein_id="HAQ0456864.1"
                     /translation="MKLKITVICSVVFSLFFFLLLFLVIFFADDTDSGENNKDSSIPQ
                     GGVTVSPEVLAHRPLIEKYGKEYGIEDYVSYILAIMQVESGGTAEDVMQSSESLGLPP
                     NSLSTEESIKQGVKYFSELLTSAEQQGVDIDSVIQSYNYGGGFLNYVRSHGKKYTYEL
                     AEQFSKEKSGGQKADYPNPIAIPVNGGWRYNYGNQFYVQLVSQYLTDTSPTEFDDETV
                     QVIMDEALKYEGFPYVFGGASPTTSFDCSGLIQWVYDKAGISLPRVAQDQYDATQEIS
                     MEEAQAGDLIFFHSTYNAGTYVTHVAIYLEGNRFYHAGDPIGYGDLSSRYWQDHLIGA
                     RRVIHN"
     gene            13591..14520
                     /locus_tag="IWO89_002143"
     CDS             13591..14520
                     /locus_tag="IWO89_002143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017650613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HAQ0456865.1"
                     /translation="MIIKIERKEKKEKPKKSKKPSKQRKVPMIKVGTHKKLTFVLWVL
                     LIGSVGFGIYKNFTAIDTHTVRETEIIKQQIVDTNQVESFVKSFAKDYFSWQQSQEAI
                     DKRNEKLTHYLTEELQVLNEEMIRKDIPTSSSVNDIQVWQVSQVNENTFEVLFSVEQV
                     ITEDKDKETISSSFHVVVHIDESDNMVIIKNPTMSKKPQKSDYQPKQLESDHTVDTET
                     MDEITSFLETFFQLYPTATEKELTYYVSNHVLPMINKEYVFEELVNPIFTRKDNQVIV
                     NVAVKYLDQETKATQISQFELILEKQDNWKIVK"
BASE COUNT         4997 a         2454 c         2906 g         4333 t
ORIGIN      
        1 taaaaaatga atttggaata gaagttaaag attattttat tgacttagat ttattacagc
       61 taataaacag tgtgaaaaga atgtatagtg gagacgacaa cggtgttgat ttatacaaat
      121 ttgttaccaa acatatattt gttaatctta atattgacga gaaaaattac ccgtctattt
      181 ttaagcacta tctatttttt attaacgaag tagaagatcg gataaaaaat aattcattat
      241 ctagtagtgt atctagtttt aagaagatgt ttaaatctcg agatggtatc acacttacta
      301 cagcacataa agttaaagga aatgagtatg atactgttat cgctattaat cttctaaacg
      361 ggaaaattcc tcattggaat gaaattttta atgtttctga taaaggaatt agttcagcga
      421 gaaaactact atacgtaatt tgttcaagag caaaaaataa tttatttctt ttctcagagg
      481 tgggttattt tacgagtaaa ggagacttgc tttatcccac tcaattatta gataaaatag
      541 aatttgatta taatatttaa aatacctagg tagtcgttgt tttaaatata gtaggaatcg
      601 gacgctcagt acggagcgtc tattttttta cccaattttg aaaggaagtg aacttatgaa
      661 aataaaaaat caagaatcaa aaggtcgctc tccccccttt aaaaccatca cacaaataag
      721 attcattcaa acataaaaac agaaaggata ggtaaaaata tggaacttaa atatgtcatt
      781 cccaatatgg aaaaaacatt tggacaatta gaatatgctg gtgaagggaa cattgaacaa
      841 cgtcgaataa atggcagacc aaccattctc tctcgtagtt acaatttata ctcaacgatt
      901 caacgagcag atgatattgt tgtcattctt ccctccgaag ctggcgagaa acattttgaa
      961 tccgacgaaa aagtcaaact catcaaccca catattaccg cagaaggtta taaaattggc
     1021 aatcgaggat ttaccaatta cattatgcac gcagacgata tggtgaaagc ataagaaagg
     1081 aaaggtgaaa aaatatgaga ttagcagaag gtattgtgat tgaaaaagac cagacatttg
     1141 gcacattgaa attttccgcc ttgcgtcgtg aggttcgtat ccagaatgaa gatggtacgg
     1201 tttccgaaga aattaaagaa cgtacctatg acttaaaatc aaaaggacaa ggacgcatga
     1261 tacaagtcag tatccccgct tctgttcctt tgaaagaatt tgactacaac gcagaagtgg
     1321 aattaatcaa tcctgtggca gataccgtag cgacggctac ttttcgaggg gcagacgttg
     1381 attggtacgt gaaagcagac gatcttgtat taaaaggaca gacaaaacct catcagaaat
     1441 ataaaaaaga agcgtccgaa agtgaaaaat aggtgttgct tatgacaaac acaccattga
     1501 ttttgaaaga aattccgaag gatgaagcca tttcttttat tcggcaatac cattattcaa
     1561 agatactccc tagattgtgt aaatactttc tagggatttt ttcagaagaa aaattattag
     1621 gcgttgtgga attaggctgg ggtacgcaac ccctacaaac catacgcaaa ctctttcccg
     1681 acagttccct tcaaaccaca gattatttag aaattggaaa gatgtgtttt cttccagaaa
     1741 tgaatcaaac caattatttt ggcagtcaag ctctttcagc tctaattaag tggttgaaag
     1801 aacatacgga ctgtcatttt ttatatacac tggcagatgg aattgaggga aaatgtggct
     1861 atgtctatca agcgtccaac ttcttttact gcgggtattt caagacaagt gtctatcgtg
     1921 ataagcagtc atgggaaaag atacaccctc gaagtgctcg ccttttatta gaggaaaatg
     1981 ctcgctttga acaggtagag aaaaagcatt ggctttccca ggcattttgc gaatacaaag
     2041 ggattgagaa aatcaatgga cgcatgtttc gctatctcta tccgttgaca aaagaagcga
     2101 aaaaactgtt aggacacact ctctatcgac gtcattatta tccgaaagaa aaaaatttac
     2161 gctttgaaaa gcgaatcgct tatcagaaat atgaagccat ttctcaacca acttttgata
     2221 aacaggctcg tatctacaat acacaattat tttgataggt ggaaaggagg atttttatgc
     2281 cattgcttca tgacagagga gagcgaatca aagccaacga taagcacttg gtctatcatt
     2341 ttgtactaat ttcattgctg gtagtttttc tagtcgtcct actccctttt cattggagag
     2401 cgttacaatc ggtgaactgg caagcagttc agttagatac atttttctat caacttcata
     2461 cgccatactt ttggagcagt attctcactg ctctttttgt atgcggactt atcagctggc
     2521 tttattatca ctatcgtata gattctataa aacagttaga gcaccgtcaa aagttagctc
     2581 gtatgatttt agaaaatcat tggtatgaag ccgaagaaac tcaatcagag ggattcttta
     2641 aagatttacc cagcaagaaa accaaacaac gaatccgcca ttttcctaaa atatattatc
     2701 gaatgaaaga tggactaatc catattcatg tagaaatcac cttagggaaa tatcaagatc
     2761 agcttttgca cctagaaaac aaactagaaa caggattgta ttgtgaatta gtggaaaaaa
     2821 cgttgaaaga ttcttatgta gagtatgtcc tactttatga tactattggc aatcgaattg
     2881 gcattgaaga cgtggtagct caagatggac gtgtcaaatt gatggaatcg gtctattggg
     2941 agttcgattc tctccctcac atgttaattg ctgggggcac tggtagtgga aaatcctatt
     3001 ttattctcac attgattgaa tctctcctgc atacaaatgc aaagctttat attcttgacc
     3061 ctaaaaatgc tgatttggca gacttagggg cggtcatgaa taatgtctat taccgtaaag
     3121 aagatatgtt ggcatgtatc aatcaatttt acgaagatat gattgatcgc agtgaaacca
     3181 tgaaacaaca ccctaactat aaaacaggag aaaattatgc ttatcttggt ttgtctgcca
     3241 attttcttat ttttgatgaa tatgtggcaa tgatggatat gctaggtcgt gaaagtacca
     3301 ccgttattca taaactcaaa caaattgtaa tgttaggacg acaagcaggc ttctttttaa
     3361 ttcttgcctg tcaacgtcca gacgcaaaat attttagtga tgggattcgg gacaacttta
     3421 acttccgtgt ggctttgggt cgtatgtctg aaatgggctt tggtatgatg ttcggaagcg
     3481 atacacaaaa acaatttttc ttaaaaccca tcaagggaag aggttacgtg gatacaggaa
     3541 aaagtgtgat tagtgagttt tatacacccc ttgtaccaaa aaggtacgat ttcttaggag
     3601 aaataggaaa agtcattcaa aaaaagcaat ctgagcctgt accgcacgaa gtgaaaggta
     3661 caggttcaga cttgctttga ttggtgtggc gttagccacg ccaatccttt agccccctct
     3721 atctaacagg ggggcacaaa tacaactgaa aacaggcaat tctcatcaac caatgttgat
     3781 ggtacaaagg attgcggaaa atatgaaagg tgtaaactca agggtcatag tttacatctt
     3841 ttttagattg gaggtttgcg aatggaaaac gaaaaaattt ggattaagga attgaaagaa
     3901 aagcgactgg cttatggcgt ttctcaaaat aaactggcgg ttgcttctca tattaccaga
     3961 ccttacctat cggatattga aacaggcaag gcagtcccta ctcaacaggt gaaagaagat
     4021 ttactgaacg ctttggaacg cttcaatccc gataacccat tggagatgtt attcgactat
     4081 gtacggattc gattccctac caatgatgtg gctcaaatta tcggtgaggt tttacgtttg
     4141 aatatggatt atatgctaca cgaagacttt ggctattatt cttatcccga acactatcgt
     4201 ttcggtgata ttgttgtcct agtctctcat gatgtaagta aaggtgtttt attagaactg
     4261 aaaggaaaag gctgtcggca atttgaaaac tttctattgg ctcaacatcg gagctggtat
     4321 gatttctttc aagattgcat ggaacataaa ggtattttca aacgcttgga tttagctatc
     4381 aacgataaaa cagggatttt gaatattcca gatttggcaa agaaatgtaa acaggaagaa
     4441 tgcatttccg tctttcgaag ctttaaaaat tatcgcagtg gtgaattggt tcaccgtgat
     4501 gaaaaaccat acatgggaaa cacactttat attggctcat taaaaagtga agtctatttc
     4561 tgtatatatg agaaagatta tgagcaatac gtgaaaaatg atgttccctt agaagacgca
     4621 gaagtaaaga atcggtttga aattcggtta aaaaatgacc gagccaccca agccatgaaa
     4681 gatttattgg cacatcaaca ggcggaaaaa acagcctttg aaattatcaa tcgctatatc
     4741 cgctttgcag ataaagacga cactaaacgt cggagtgatt ggaaaactaa tgaacgttgg
     4801 gaatggttta tcggtaaaaa tcgtggagca ttacgcctaa ccacacagcc agagccttat
     4861 tcttttgaac gaacattgaa ctggttacat catcaagtcg cccctacatt aaaaatcgca
     4921 agcattttag atgttctcaa cggtacaacc attatctcca ccatgattca ggaagccaaa
     4981 ctgacagaaa aacatgagaa attaattgaa caacaacatc tagccatgga ggatttgatt
     5041 acatgagcct tttaacaaaa gaattaaaga agttagggtt tcagtgtggc attgagtttc
     5101 aagcgtatat acaaaataca gggaaatata cgtctctcat tattgaggga aaacggcaag
     5161 ctggtgatac catctatacc tatgattttt acaaggtgag attttataat aattatacca
     5221 atcgagtaac cgtctatgga gagcatttaa ccccttttca attattaagg cgtgtaaaaa
     5281 gctatatcta ttatcgagaa aaatatctta aggaaaggag gacaatcaca tgaattttgg
     5341 gcagaatttg tacaactggt tcttggataa tgcacagtca ttggtattgt tagcgattgt
     5401 tgtcattgga ctgtttttag gttttaaacg agaattttca aaactcattg gcttcttgat
     5461 tattgctcta atcgccgtcg gacttgtgtt caatgcagct ggcgtgaaag atgtgttatt
     5521 aaacttattt aaccgaatca ttggtgctta acagatattt ttcatgataa aataggaagt
     5581 atatattttg ttatgaaagg atttatgaat atgtttacaa atgatcaatt taaatctctt
     5641 ttaattaaaa cattagaaag aaagagctct tcaaattatt atgaaggagg taacgagatt
     5701 gtttctaaaa tgaaaatatc agaggtaact ttagatgaga ctgacgattt tacttattat
     5761 aaaggatata aaaagggctg gaatacgctt tgcacatatc ttaaaataag agttcctttt
     5821 gatgatttag acttttttga aagtcataaa gatttgatta cccagacagc aagctccata
     5881 tatgataaac aaggtgataa tgtattagtg gatactattt tagtaccact tccagagaat
     5941 tacgaagtaa ttaactttag tcagttgaaa attagtgatg tggtttcaca agctattgaa
     6001 gacgctgagt cttttatgag taatggtgaa tatcaacgtg cctttgacag agttcacact
     6061 gcttttcatg gatatttaat tgaaatacta aaaaaatatg aaattacagt tcctagagac
     6121 gaaaatctat ctaaattata tagtaggata caacaattaa ttgaaaaaga aattcaacct
     6181 actgaacttg ctgatatagt gaagacaacg ataaggtcat caaatggaat gattagctct
     6241 cttaatgaag caagaaatag gcattcactt gcacacccta atacaaatat tattggaaag
     6301 agagaagcaa aactaataat aggtatttca agtacagtta cagattatat tagcggatac
     6361 cttgataagt agcgtcatta actgattgct acttttttta tgactatttt tataaaaagg
     6421 atgtgataag aattggaaat gaaagtctat gtggcaaatt taggacggta taatgaaggt
     6481 gaattagtcg gagcatggtt tactccacca attgatgagg aagaaatggc agaaagaatc
     6541 ggtttaaatg aggactacga agaatatgca atacatgatt ttgagcttcc ttttgatgtg
     6601 gacgaataca cgccgattag tgaaatcaac cgtctatgtg aagccattca agaaatcgaa
     6661 ggtacaccta tttataatga actcaaagaa attcaaggca tgtggtttag tagcttggaa
     6721 gaattactgg aaaacaaaga ggatattcat tgttatagtg attgtgattc gatggaagat
     6781 gtcgcccgtt attacgtgga agaaacagga cagcttggtg aagtcccttc caatttacaa
     6841 aattatattg actaccaatc tttgggacgt gatatggaaa tagagggtaa ttacctagtt
     6901 acctctcatg gggtatttga atattgtcag taagtttaac aatccgtgtt ataattaaat
     6961 taataaatag cacggagagg tataaattat ggtaaatgta tttgaaaatc ttaaactatt
     7021 aaaactagac atgaaagata aaggctgggt gatagattcc ttttactttc gttacaaaca
     7081 gcaaaattat attgtacttg ttaagctatt tgaaaaagag gaaaaagttc cagaatatgc
     7141 tctgttaaaa ctagaatttt taaaggaaaa cgatttttca gatatgctag ctgtctatgc
     7201 taattcggta aaattatata cagacacgaa aacaataaga gaatattttg gtatcgaata
     7261 cagtagtaac ttgggagatg ttttatttca atttagccaa actcttgctc gatttattcc
     7321 gacagaagtt agtgagaaga aaaatgaaga ccaaaaagaa gccatgtgtt tctcattaag
     7381 tcaaagtgat tcagaagatc ccagaaaaaa atattgtttt tcagttagaa gaaatccttt
     7441 aaagggaaat ggtgagctgg gaaaacgaag tccttttaat gacaataaaa cgagattgta
     7501 tagacccagc ttatatgaac gattagggtc agataccaat ttaagtttca gatattcaat
     7561 gaatccagat gatgaagaaa ctgatgaagg tattattgca aaatggacaa aaaataaaat
     7621 tgaatagtta aagcattgaa agagcaatca cctattttga ttgttctttt tctattttta
     7681 cggaaaggac gtgaagacat gaaaaaaata agaagttaca ccagtatttg gtcagtagaa
     7741 aaagtgattt atgcgattaa tgatttacaa ctccctttcc caattacctt tacgcaaatg
     7801 acatggttta tcgtttcttt gtttgtcgtc atactctttg gcagtttacc accactttct
     7861 ctgattgacg gagcactact caaatactta gggattccca ttgttctcac tgggttcatg
     7921 tgccaaaaga cctttgacgg aaagaaaccc tatggatttc taaagtctat cattacctat
     7981 gctctacgcc ctaaagtaac ctacgctggc aaacaaatac gattaaaaaa agaaacaatg
     8041 aatgaatcga ttacgattgt aaggagtgaa caactagatg gcatatccga ttaaatatat
     8101 ggaagataat ctcgtattta atcatgatgg cgaagtattc gcttattatg aattaattcc
     8161 ctataattac agttttttaa gttccgatga aaaaattcaa gttcatgata atttctgtca
     8221 attgattgcc caaaatcgtg atggcaagat tcatgctctg caactagcaa ccgaatcaag
     8281 tattcgagcc acacaggaac aatcgaaaaa agaaatcact ggacgattaa aagagatagc
     8341 ttacgaaaaa atagatgaac agaccgaagc gttaatttct atgattggtg acaatcaagt
     8401 agactatcgc ttttttattg gttttaaact ctacctcaat gaacaggaag tatccgtaaa
     8461 aaatgttggg aaagaaattc aaaaagcatt tgcggaattc tttcatggtg tgaatcacaa
     8521 attaatgggc gattttgttt ctatgcctat ggcggaaatc gaacgctttt ctaagatgga
     8581 acagttgtta gaaagtaaat tatccagacg ctttcagatt cgtaaattgg agaaagatga
     8641 ttttggttat attattgaac atctttacgg gcaaacaggt gtcccttttg atgactacga
     8701 ctaccattta ccgaaacgat acgaagataa aaaagtgttg gtaaagaaat acgatattct
     8761 caaacccact cgctgtttaa tcgaagaaaa tcaacgctat ttacggattg aacaggaaga
     8821 taccactact tatgtggcgt attttacaat taatagtatt gttggtgaat tggatttccc
     8881 taatagtgaa attttttatt accagcaaca gcagtttact ttccccgttg ataccagtat
     8941 gaatgttgaa attgtcgcca ataaaaaagc cttatctacc gttcgcaata aaaagaaaga
     9001 actgaaagat ttagatagcc acgcatggga aagcgacaac gaaaccagta ctaatgtggt
     9061 ggacgcttta gaatctgtcg atgaattaga aagtaattta gaccaaacca aagaatctat
     9121 gtacaaacta agctatgtgg tacgggtatc agcaaagaat ttggacgagc tgaaacaacg
     9181 ttgtgatgaa gtaaaagatt tttatgatga tctgtctatc aaattagtac gaccttttgg
     9241 cgatatgcta ggcttgcata atgaatttat tccctcaagc aagcgatata tcaatgacta
     9301 tattcaatat gtcacgtcgg attttttggc tggtcttggt ttcggtgcta cacaaatgat
     9361 tggtgaaaat tctggtattt acgtcggtta caatctcgat acaggcagaa atatttatct
     9421 ccaacctagc cttgcaagtc aaggaattaa aggctctgta acaaatgcat tagcgtccgc
     9481 ttttattggt tcattaggtg gcggaaaatc attctctaac aatatgctgg tctattattc
     9541 agtactcttt ggcggacagg caaccattgt tgacccaaaa gctgaacgtg gtgaatggaa
     9601 agaaacctta cctgatattg cccatgaaat caatattgtt aatctcacga gtgagaacga
     9661 caataaaggg ttacttgacc cttatgtgat tttaaaactc ccgaaagatt cagaaagcct
     9721 tgccattgat attctcacgt tcttaacagg tatttccagc cgagatagcg agaagtttcc
     9781 tgtcctgcgt aaagctattc ggagtgtaac tcaaagcaaa caacgagggt tgttattggt
     9841 gattgatgaa ttgcgaaaag aagatacgcc catcagcaat agtattgctg accatatcga
     9901 aagctttgtc gattatgatt ttgcccatct gttattcagt gatggcagtg tcacccaatc
     9961 cattagcttg gaaaaacaac tcaatattat tcaagtggca gatttggtct tacccgattc
    10021 caatagctct tttgaagaat acactaccat ggaattatta agtgtggcaa tgctgattgt
    10081 cattagtacc tttgctttag actttatcca ctctgaccgt tccattttta aaattgttga
    10141 ccttgatgag gcttggagtt ttttacaagt ggcacaagga aaagctctat ctatgcgatt
    10201 ggttcgagca ggacgtgcca tgaatgcagg ggtctatttc gttactcaaa acgcggacga
    10261 tttactagat gaaaagatga agaataatat tggcttgaaa tttgcctttc gttccactga
    10321 cctagtggaa atcaaaaaga ccttagaatt ttttggtgtg gacaaggaag atgaaaacaa
    10381 tcagaaacgc ttacgtgaat tagaaaatgg acaatgttta gtttcagaca tctatggtcg
    10441 tgtaggggtt atgcagtttc acccgatttt tgaagagttg cttcatgcct ttgataccag
    10501 accgcctgtg cgaaatgagg tggaagaatg acacaaaaac aaaagaaatt tcttcactat
    10561 agttggataa cgctcctttg tgtgggcgtt attttacttt tattaggcac gctgggcaat
    10621 gcagtacaag cgacaggctt agttgatgaa accattgata caagcaatga atactccaag
    10681 tatggtttga atcaatatca gcttgattat tatgtggaca atagctgggg ctggcttcca
    10741 tggaattgga gtgatggcat aggacaatcg gtgatgtatg gattgtatgc cattactaat
    10801 tttatttgga caatttctct ttatctatcg aacgccacag gttatttggt gaaagaagcc
    10861 tatgccttag atttcattag tcaaacatcc caagcaattg gcgaaaacat gcagacgatt
    10921 gcaggcatta caccgaatgg cttttccagt tcagggtttt atgtcggttt cctccttctt
    10981 tttattctca ttgttggaat ctatgttgcc tatacaggat tggttaagcg agaaacgacg
    11041 aaagtgatac gagctgtttt aaactttgtc gtggtgttta ttctatcggc ttcctttatt
    11101 gcgtatgcac ccgattatat tgcgaaaatc aatgattttt ccaaagatgt cagtaccgcc
    11161 agtctgaata ttgggacaaa aattgttctt cctaacacgg atagtcaagg gaaagacagt
    11221 gtggacatga tacgtgacag cttattctct gttcaagtaa agcaaccatg gctattgctt
    11281 caatatggct ccactgatgt agaagcgtta ggggaagaac gtgtggaaag tttattgtct
    11341 accagtccag atacaaacaa tggtgaagat agagaaaatg tagtgattga agaaattgaa
    11401 gatcatgaca atacctattt aaccctaccg aagacgattt ccagattagg aacagtcttc
    11461 tttttgtttg tattcaatat tggtatctcg atttttgttt tccttctaac aggaatcatg
    11521 attttttcgc aagtgctatt tattattttc gctatgtttt tgccaatcag tttcctatta
    11581 agtatgttgc cgagttttga tggcatgggc aggcatgcca ttaccaagtt atttaatgtg
    11641 attatgacaa gggctggtat taccttgatt attaccgttg cctttagtat ttccactatg
    11701 ctttattcgc tgtcagcaag ttctcccttt ttcatgatta tgtttttaca aattgtgacc
    11761 tttgcaggga tttattttaa actgggcgat ttaatgagcc tattcacttt acaaagcaac
    11821 gactctcaaa acatgggcag acaagtgttt cgcagacctc gtcaaatgat gaatcgacaa
    11881 accagaagat ttcaacgcag tatgagtaag atttttcgtg gaaaagcagg gtcaacgaag
    11941 caaaagacaa tgacaacaaa gcagaatcaa accaataaag ctaaccatac tcgtcaaaat
    12001 gaacggacta caccaaagaa agaaacagca ctttccaatg tgaaaaagca acaaggcggt
    12061 atcgggcaac gattaggtga aaagacagcc catgtattgg acacgaaaaa tcgtctagtc
    12121 aataaaagaa agcaggcaaa acaacagata caggcgacac caacccatgt caaatatgcc
    12181 cttcatcaag gaaaagagaa tgtcactcat aatgtaacag actttaaaca atctatggca
    12241 gataccaaag ctcaacgcaa acaacagcgt atgaaccagc aggagcaaaa acgaaagaac
    12301 attgctcaaa agcgtttgga gctggataaa gcaaaagaaa aaagacgaaa agaaaaccca
    12361 tcatctcctg ttcagaataa ccagcagcat gttcgcaagc gtcagcaaac cgtcacgcct
    12421 gttttgtcac aaaacactaa acgtgaaaat catcagcgac cattgaaaaa agagcaaaaa
    12481 caaaagacac caccgaagca attcaatcag aggaagctaa ataaaagaac ggataagcga
    12541 ggacaacaaa catgaagcta aaaataacag tgatttgtag tgtggtcttc tctctcttct
    12601 tttttctctt attattccta gtcattttct ttgcagatga tacggacagt ggcgaaaaca
    12661 acaaggattc ttctattcca caaggcggtg tgaccgtttc accagaagtg ttggcacacc
    12721 gtccgttgat tgaaaaatat ggtaaggaat atgggattga agactatgtt tcttatatct
    12781 tagccattat gcaagtggaa tcgggcggta cagcagaaga tgttatgcaa agttccgaat
    12841 ctttgggttt accgcccaac agtttgagta ccgaagagtc catcaaacaa ggggttaaat
    12901 atttcagtga attattaacc agtgcagaac aacaaggagt ggatatagat tctgttatcc
    12961 aatcttataa ttatggcggt ggctttttaa attatgtgag gagtcatggg aaaaaatata
    13021 cctatgaatt agccgaacag ttttctaaag agaagtcggg tggtcaaaaa gcagactatc
    13081 ccaatcctat agccatacct gtgaatggtg gttggcgata taactatgga aatcagtttt
    13141 atgttcagtt ggtttcacaa tacctaaccg acacttcccc aacagaattt gatgatgaaa
    13201 ccgtgcaagt cattatggac gaagccttga aatacgaagg gtttccctat gtctttggag
    13261 gggcgtcgcc taccacctct tttgattgta gtggcttaat acaatgggtg tatgacaaag
    13321 ctggcatttc tctccctcgt gtcgcccaag atcagtatga cgctacgcaa gaaatctcta
    13381 tggaagaagc ccaagcaggc gaccttatct tctttcattc aacctataat gcaggcacat
    13441 atgtgactca tgtggcgata tatttagagg gaaatcgctt ttatcatgca ggcgacccaa
    13501 tcggttatgg cgacttaagc agtcgttatt ggcaagacca tctgattggg gctagacgtg
    13561 ttattcataa ctaagaaagg aatgaatcaa atgattataa aaatcgagcg aaaagaaaag
    13621 aaggagaaac caaagaaatc taaaaaaccg agcaagcaaa gaaaagttcc gatgattaaa
    13681 gttggcacgc ataagaaatt aacctttgtt ttatgggttt tattgattgg tagtgtcgga
    13741 tttggaatct ataaaaattt tactgccatt gacactcata ctgtccgtga aacagaaatc
    13801 attaaacagc agattgtcga taccaaccaa gtagaaagtt ttgtgaaatc ctttgcgaaa
    13861 gactactttt cgtggcaaca atcccaagaa gccattgata aacgaaatga aaaattgacc
    13921 cattatttga cggaagaatt gcaggtatta aatgaagaaa tgattcgtaa ggatattcct
    13981 actagttcta gtgtgaacga tatacaggtg tggcaggttt ctcaagtgaa tgagaacacc
    14041 tttgaggtac tgttttctgt tgaacaagtt attaccgaag acaaagacaa ggaaactata
    14101 tcgtctagct ttcatgtggt tgtccatata gatgaatcag ataatatggt tattatcaaa
    14161 aatccgacaa tgagtaagaa acctcaaaaa tcggactacc aacccaaaca gctagaaagt
    14221 gaccatacag tagatacaga aacgatggac gaaatcacta gctttttaga aacgttcttc
    14281 cagctctatc ctaccgccac agaaaaagaa ttgacttact atgtaagtaa tcatgtgtta
    14341 cctatgatta acaaggaata tgttttcgag gaattggtga atcctatctt taccagaaaa
    14401 gataaccaag taatcgtgaa tgtcgctgtg aaatatttag atcaagaaac aaaggcaacc
    14461 caaatctcgc aatttgagtt gatattagaa aaacaagata attggaagat tgtaaaataa
    14521 caaatattgg tacatgatta cagatacttt gtaatcatgt actctttttg ataaaaaatt
    14581 ggagattcct ttacaaatat gctcttacgt gctattattt aagtgactat ttaaaaggag
    14641 ttaataaata tgcggcaagg tattcttaaa taaactgtca atttgatagc
//
LOCUS       DACEEZ010000071        14522 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20313_2017
            SAMN12639966-rid9879223.denovo.071, whole genome shotgun sequence.
ACCESSION   DACEEZ010000071 DACEEZ010000000
VERSION     DACEEZ010000071.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639966
            Sequence Read Archive: SRR10023158
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 14522)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14522)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741683.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741683.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:44:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,016
            CDSs (total)                      :: 2,962
            Genes (coding)                    :: 2,889
            CDSs (with protein)               :: 2,889
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 36 of 73
            Pseudo Genes (incomplete)         :: 29 of 73
            Pseudo Genes (internal stop)      :: 18 of 73
            Pseudo Genes (multiple problems)  :: 8 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14522
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639966-rid9879223.denovo.071"
                     /isolate="E20313_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(327..689)
                     /locus_tag="IWO89_002144"
     CDS             complement(327..689)
                     /locus_tag="IWO89_002144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0456866.1"
                     /translation="MASKRVAGTIMLHLEDGTKKFLVHSIDDKLEFALAELSEGKTGL
                     ANILNFLKEIVHIDVSKISLMELTNTHIKEENVPLFVFETEQKNQREELGEGYIWEEP
                     GQMRSVLGTYEVEGVPFF"
     gene            complement(763..1143)
                     /locus_tag="IWO89_002145"
     CDS             complement(763..1143)
                     /locus_tag="IWO89_002145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S1 RNA-binding domain-containing protein"
                     /protein_id="HAQ0456867.1"
                     /translation="MTYKIGQIIEGKVTGIQPYGAFVSLDNETQGLIHVSEVQSGYTK
                     NIHSLLKVGQPVTVQIIDIDEYSKKISLSLRTLEKTNPAVPYRRKRYFTNKNRKIGFT
                     TIAEQLPIWIEEALSELERKENKK"
     gene            1225..2400
                     /locus_tag="IWO89_002146"
     CDS             1225..2400
                     /locus_tag="IWO89_002146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HAQ0456868.1"
                     /translation="MIEFDKQISRKDTESVKWDAIAETYQMDDLLPLWVADMDFLSPG
                     GVAKAFSEYIKHGIFGYATLSDKLYQAIIHWERQHHAVELTKENIVFTSGVLTSLAAA
                     VQTFTKPGDSILIHDPVYPPFSSIIETNQRHIVRSSLLEKNNHFIMDLSDMEKKIEEN
                     NVKAMILCNPHNPGGRVWSKEELKQLSELCLKHQVILFSDEIHQDLTLFDHTFTSMLT
                     IDPNLDDLLVAFTSATKTFNLAAIKNSMVFIKNPEMKADFEKHLVMNQQHEINTFGLI
                     GTQAAYETGGEWLSQLIPYLEKNVQTVNQFFEQYLPKVRIMNPEGTYLMWLDFTAYIS
                     DDQELENTLVRKGKVVLNPGITFGPSGHGHMRLNIACPEETLLEGLNRIKYALETID"
     gene            complement(2477..3061)
                     /locus_tag="IWO89_002147"
     CDS             complement(2477..3061)
                     /locus_tag="IWO89_002147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HAQ0456869.1"
                     /translation="MAFPQLDLKSEKGPKAVIKTNRGDITVQLFPELAPKTVQNFIEL
                     SKKGYYDGVIFHRVIPDFMIQGGDPTGTGMGGESIYGESFEDEFSRELFNLRGALSMA
                     NSGPNTNGSQFFIVNNTNVPANMLGQLEGAGFPSEIIEAYKGGGTPWLDFRHTVFGHV
                     LEGMDTVDEIANVQRGPQDRPVHDVVIETIEISE"
     gene            3262..4260
                     /locus_tag="IWO89_002148"
     CDS             3262..4260
                     /locus_tag="IWO89_002148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HAQ0456870.1"
                     /translation="MEIYDITIIGAGPVGMFAAFYAGMRQAKTKIIDSLPQLGGQLAT
                     LYPEKYIYDIPGYPAIKASELIDQLEKQLTTFNHTFHLKEEVLSLTREDEVIEITTNK
                     GIHYSKAVILALGNGSFQPRKLNLDNAESFENHGLDYFVNDLMKYAGKKVAIAGGGDS
                     AIDWTLMLEPIASEVYLIHRRPEFRAHEHSVSRLKSSSVNLLTPYLIDGLSGNNGELT
                     DIRLKKVKSDETIDLMIDSLIVNYGFSSNLEHLSSWGLDSTRNAITVKSDMSTSIPGV
                     YAAGDICTYEGKVKLIATGLGEAPTAVNNALHYINPKERTQPGHSTSLYDKNLRPS"
     gene            4372..4881
                     /locus_tag="IWO89_002149"
     CDS             4372..4881
                     /locus_tag="IWO89_002149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase A"
                     /protein_id="HAQ0456871.1"
                     /translation="MVVKTETLEEKARELLKERGVTVEDIAQLVMFLQKDYISDLTLA
                     DCTESVNSVLTKREVHNAIITGIQLDILAEKKKLMSPLQHIIEDDEGLYGIDEILALS
                     IVNVYGTIGFTNYGYIDKVKPGILKELNSHDGVHVHTFLDDIVGAIAAAAASRLAHKE
                     PEKRSRLTQ"
     gene            complement(4911..5570)
                     /locus_tag="IWO89_002150"
     CDS             complement(4911..5570)
                     /locus_tag="IWO89_002150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01906 family membrane protein"
                     /protein_id="HAQ0456872.1"
                     /translation="MKNSRWQWMEYAGLFSLFLTLISLAVGVTINFRPLYVFDIGHLQ
                     ILDYTSLDQETLLKNFDHLMNYLNNPFKTILSLPDFPVSASGAHHFYEVKILFLVDYA
                     VFFITLIPSILFIKYLQKNDRLWRLIRPFQIGMLLPVVFGFFMMIGFDRFFILFHETF
                     FNNDDWLFDPVTDPIINVLPEEFFMHSFILFFVLLELFFAVFLFLGKNSLKQTKKKEL
                     V"
     gene            complement(5572..6336)
                     /locus_tag="IWO89_002151"
     CDS             complement(5572..6336)
                     /locus_tag="IWO89_002151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01457 family HAD-type hydrolase"
                     /protein_id="HAQ0456873.1"
                     /translation="MDYKGYLIDLDGTIYRGTQPIPAGKRFVEELQKRKLPFLFVTNN
                     TTKSPETVANRLADEFDIHVAPETVYTATLATIDFMKADGKGKKVYVIGEAGLIDLIL
                     AAGFTWEEEAPDYVVVGLDNYLTYEKVVKATLAIQKGATFIGTNPDKNIPTERGLLPG
                     AGSVISFVETATQTPPIYIGKPEAIIMDKAVEVLGLQKEEVIMVGDNYETDIQAGIRN
                     NIDTLLVLSGFTKEEDVPGLPIPATYVKQSLDEWSF"
     gene            complement(6344..7021)
                     /locus_tag="IWO89_002152"
     CDS             complement(6344..7021)
                     /locus_tag="IWO89_002152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YutD family protein"
                     /protein_id="HAQ0456874.1"
                     /translation="MSAKQNEQQNVEHKTEIAKKKSSAEQETTVTEELTAALEEIVEE
                     PKAEKVKGELVTLLDDSNFLIGERHYRLVSDYREGFNAEKLGERYSDVLARYDYIVGD
                     WGYEQLRLKGFFQADNRRAHPDQRIDSLEDYLYEYCNFGCAYFVIERIGGKKEKTTQR
                     RKKKKNHNRAYIDEKKGPVASNRKKPVIKNRKTESAKTSEKVKKQEQPKQVEKTKTAF
                     TIRKREE"
     gene            complement(7058..8443)
                     /locus_tag="IWO89_002153"
     CDS             complement(7058..8443)
                     /locus_tag="IWO89_002153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional metallophosphatase/5'-nucleotidase"
                     /protein_id="HAQ0456875.1"
                     /translation="MEKIVILHTNDLHSHLENWPRIRRFLDQRKRENEKKEKTTTIAV
                     DLGDFVDRWHPLSEATNGQENILLMNEVGYDAATIGNNEGVGNSKEELNHLYDHAEFD
                     IILDNLFDKNTLQPPAWTKPYKIITTKHQTKIGLIAFTAPFPLTYNPNGWDIRNPYDI
                     LPELVEDLRSQVDVLVLMSHLGIQDDFQIAKEIPAIDVILGSHTHHLFREGHVINDVQ
                     IAAAGKYGQYVGEVHLTIDEEKKILKHSAKAIPTETMTAFTEDEQEINSYLERGHELL
                     KEKEVADLPFSLSLDIFNEHSFIQVAFEAVKERGKTEAAILNSGLFLTEIPKGRVNQD
                     QLHTALPHPMHLLNVTLGGNDLIRLVLEIEKNRNFLRNYPMKGMGFRGKIFGQMVYNG
                     ITYDSINHQVFWKNKPIDLNKNYTFTTVDHLMFVPFFPTIEIAGKNEFLFPEFIRSVV
                     GDYLKAHYPIK"
     gene            complement(8653..8737)
                     /gene="ffs"
                     /locus_tag="IWO89_002154"
     ncRNA           complement(8653..8737)
                     /ncRNA_class="SRP_RNA"
                     /gene="ffs"
                     /locus_tag="IWO89_002154"
                     /product="signal recognition particle sRNA small type"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00169"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00169"
     gene            complement(8760..9275)
                     /gene="tadA"
                     /locus_tag="IWO89_002155"
     CDS             complement(8760..9275)
                     /gene="tadA"
                     /locus_tag="IWO89_002155"
                     /EC_number="3.5.4.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA adenosine(34) deaminase TadA"
                     /protein_id="HAQ0456876.1"
                     /translation="MVNTNLDAVEKEKWMRLALAEAKKAEMLHEVPIGAVVVLDGKVI
                     GRGYNLRETTQDATTHAEMLAIKEACEKVGSWRLEDAALFVTLEPCPMCSGAMILSRV
                     AEVYFGAYDPKGGTAGTLMNLLEDERFNHQAYVEGGILEDECGMILTDFFRKLRQRKK
                     KLKKDWSTNEK"
     gene            9382..10239
                     /locus_tag="IWO89_002156"
     CDS             9382..10239
                     /locus_tag="IWO89_002156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fis family transcriptional regulator"
                     /protein_id="HAQ0456877.1"
                     /translation="MKMDKLLALYPQAQKKNSPASEADILSFAIDDAFIWIKQDSLSQ
                     QETSLLKALFPVINDQKKHPWYRYLFKDASCVQEKSFRLIQLHVESKGEFLKKEWQDT
                     ILELFRYSADFFFYTDTDALLVEQINRDYLDATEINGIFLSLDADFDTTTSVFVGSFH
                     SPGTDLVPLFAEERKIFLTEQNNLYTHSRTFSMQDVALHYYTKDTVKDSKLIKSYIEL
                     IKQNEMEVIIQELWNELGNVSSAAKTLFLHRNTLKYRIEKFQEQSGFNLKKANDLLFC
                     YLLLLHEHN"
     gene            complement(10320..10655)
                     /locus_tag="IWO89_002157"
     CDS             complement(10320..10655)
                     /locus_tag="IWO89_002157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0456878.1"
                     /translation="MKTSTKVTIGLSVAAAASVATAVVVSGKVMEKIHHMTTRTKVKK
                     FVYDKFDGNEKLLEIVDGLSDSDLDSLMGMLAKIKSGKKKISVYGDSIKDSTEDVKDR
                     LMHFVEKMM"
     gene            complement(10722..12092)
                     /locus_tag="IWO89_002158"
     CDS             complement(10722..12092)
                     /locus_tag="IWO89_002158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NOL1/NOP2/sun family putative RNA methylase"
                     /protein_id="HAQ0456879.1"
                     /translation="MKEEATTLPQQFIKKYRLLLGEEASDFFSALEQGSVKKGFRWNP
                     LKPAGLDMVQTYHSEELQPAPYSNEGFLGTVNGKSFLHQAGYEYSQEPSAMIVGTAAA
                     AKPGEKVLDLCAAPGGKSTQLAAQMKGKGLLVTNEIFPKRAKILSENIERWGVSNAIV
                     TNHAPAELVPHFSGFFDRIVVDAPCSGEGMFRKDPNAIKEWTEESPLYCQKRQQEILS
                     SAIKMLKNKGQLIYSTCTFAPEENEEIISWLVENYPVTIEEIPLTQSVSSGRSEWGSV
                     AGLEKTIRIWPHKDQGEGHFVAKLTFHGQNQMHKEKKTRKKSKVQMTKEQEKLWTEFS
                     NDFHYEATGRLLVFNDHLWEVPELAPSLDGLKVVRTGLHLGDFKKNRFEPSYALALAT
                     KKIENIPCLPITQKEWQSYTAGETFQRDGNQGWVLLVLDKIPVGFGKQVKGTVKNFFP
                     KGLRFH"
     gene            complement(12170..12706)
                     /locus_tag="IWO89_002159"
     CDS             complement(12170..12706)
                     /locus_tag="IWO89_002159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HAQ0456880.1"
                     /translation="MMGRVTNYSKEYLLFKSMVYVEEYGMKSITARELAEFCGCSTYP
                     IYTHFKSISGLKEKILEEITVYFENYLAECSSDDVLSIVYLLKDFFSIHESIREIIAK
                     SDLDMDSLFKRPFFSYVKGHTKIKEEKSLELVWLNALGSLRSDDSSQDFLEMMNAVWD
                     KNEVTDEYLENILKEQSP"
     gene            13015..14496
                     /locus_tag="IWO89_002160"
     CDS             13015..14496
                     /locus_tag="IWO89_002160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M protein trans-acting positive regulator"
                     /protein_id="HAQ0456881.1"
                     /translation="MLIEARKKELLVLQMVIQKNNCSLQELSNDLHIPKRTIKELIRK
                     INVSLKQFLLIDEFIYSNHKGEIQTKETDQTKKLAVFSKLKLCYLKESNRFNYLMLLI
                     NYPQTAVPKKYLLEQLYISPSYLEKLTKQLNHSLKKFHLRILSVKNCCTLEGNELSIR
                     LYLYFFLSDTFGGNDWPFSSLELNALKMNSTDEQIEKISKQEKNIYLLLTLFILRNKQ
                     QAYFPKTSSEITELMDLLQHTHDYSEYFTSFSVSRPCEETETERAYFNYFFYCFFPNQ
                     ISFRQKQEIGFLFSTSKNLICRQVGQLMTYLTQQFPLNHLENKIPIYFYFLVLAVVFF
                     ELLGESSSSFSTLCFPEATSNHSSSFIQEKKLREKIDTIFSKNGERNQISQYLSQLIQ
                     HLLQTETKEQLLIHLQITKQFTGNYHLQSHLQQLFRKDAVKITAYSEHADLIITDQHE
                     KISDETETFYLDTTHNTVLFGKLIKAVHELYMKKGYENKTKRM"
BASE COUNT         4474 a         2890 c         2358 g         4800 t
ORIGIN      
        1 cagttatctt atcatgtcgt tagtaacatg tcaataacta ttttttaaat tttttagaaa
       61 tgtgatataa catccacttc agaatcaacg ctgttaaatt tatcatgtat ttcttccttg
      121 gtcaactata aattaagcat tactttgtga aatgattttt cgatcaatta ttattattcg
      181 ttatacaacc gttttcaaca tattttgaaa ataaaatgca ctaaaaaact atctttagca
      241 gaacatttca ttgtattttt tggaccaaac aataaaaaaa gaagtttgaa aaaaattcaa
      301 acttctttgt tcgcttaatc tgtattttag aagaaaggca ctccctctac ttcgtaagtt
      361 ccaagaacag agcgcatttg acctggttct tcccaaatat atccttcgcc taattcttct
      421 ctttgattct tttgttcagt ttcaaagaca aacaacggaa cattttcctc tttaatatgt
      481 gtatttgtca attccatcaa actaatcttg ctaacatcga tatgaactat ttcttttaag
      541 aaattaagta tattcgctaa accagtcttt ccttcagaaa gttccgctaa cgcaaattcc
      601 aacttgtcat caattgaatg gaccaaaaat tttttagtac catcctccaa atgcaacata
      661 attgtaccag caactctttt gcttgccaaa atttccacct cgatcaactt gttctatggc
      721 ttacataaaa attctagcac attttcagaa aaaatacact ttttatttct tattttcttt
      781 ccgttctagt tcagataatg cttcttctat ccaaataggt aactgctccg caatagtggt
      841 aaatccgatc ttacgatttt tattcgtgaa ataccttttt ctgcgatatg gaacagctgg
      901 atttgttttt tctaacgttc ttaaagataa actgattttt ttggaatatt catcaatgtc
      961 gatgatctga actgttactg gctgtccgac ttttaacaat gaatggatat ttttcgtata
     1021 accggattgt acttctgaaa catgaataag tccttgcgtc tcgttatcaa gagaaacgaa
     1081 tgcgccataa ggctgtatcc ctgtcacttt tccttcaatt atttgtccaa ttttataagt
     1141 catctacttc cctctcttcg catttatcat tatactgtaa atagctcaaa aaaagaagcg
     1201 acgaaaatga ctggaggtct taagatgatc gaatttgata aacagatttc aagaaaagat
     1261 acagaaagtg taaaatggga cgcaattgct gagacatatc aaatggatga cttacttcct
     1321 ttgtgggtag cagatatgga cttcttgtct cccggtgggg tagctaaagc gtttagtgaa
     1381 tacatcaaac atggaatctt tggttatgcc actctgtctg ataaactata tcaggctatc
     1441 attcattggg aacgccaaca ccatgctgtg gagttgacga aagaaaatat cgtttttaca
     1501 agtggtgtgt taaccagttt agctgcagca gtacaaactt ttacaaaacc aggcgattct
     1561 attttgattc atgacccagt ttatcctcca ttttcctcta ttatagaaac aaatcaacgt
     1621 cacatcgttc gaagctcttt gctagaaaaa aataatcatt tcatcatgga cttgtctgac
     1681 atggagaaaa aaatcgaaga aaacaatgta aaagcaatga ttttatgcaa tcctcacaat
     1741 cctggagggc gtgtttggag caaagaagaa ttgaaacagt taagcgaatt gtgtttaaaa
     1801 catcaagtga ttctgtttag tgacgagatc catcaagatt taactttatt cgatcataca
     1861 tttacttcca tgctaacgat tgatcctaat ttagatgatc tactagttgc ttttacttcc
     1921 gcaaccaaga ctttcaatct agctgcaatc aaaaattcaa tggtatttat caaaaatcca
     1981 gagatgaaag ctgattttga aaaacattta gtgatgaacc aacagcacga aatcaacact
     2041 tttggactga ttggaaccca agcggcttat gagacgggtg gagaatggtt aagccaactg
     2101 attccttatc tagaaaaaaa tgttcaaaca gttaatcagt tttttgaaca gtatcttccg
     2161 aaagtgagaa taatgaaccc tgaaggaact tacttaatgt ggttagattt taccgcctat
     2221 atttcagacg accaagaatt ggagaacact cttgttcgga aaggaaaagt cgtattaaat
     2281 cctggcatca cgtttggtcc ttccggtcat gggcatatgc gcttgaatat tgcttgtcca
     2341 gaagaaactt tactggaagg acttaatcga atcaagtatg ctttagagac aatcgattaa
     2401 atcatttatt caaaataaaa ggacgagacc ttactaggtt tcgtcctttt tcataattac
     2461 tccgtaccat accaaactat tctgaaattt caattgtttc aatcactaca tcatgtacag
     2521 gtcggtcttg tggaccacgt tgtacatttg cgatttcatc tactgtgtcc atcccttcta
     2581 gtacatgtcc aaagactgta tgtcggaaat ctagccaagg agttccgcca cctttatacg
     2641 cttcaatgat ttctgatggg aatccagcac cttctagttg tccaagcata ttagctggca
     2701 catttgtgtt atttacaata aagaattggc ttccgtttgt gtttggacct gaatttgcca
     2761 tagataaagc tccgcgaagg ttgaacagtt cacgactgaa ttcatcttca aaactttcgc
     2821 cataaatact ttcgccaccc attcctgttc cagttggatc gccgccttgg atcatgaaat
     2881 caggaataac gcggtgaaaa atcacgccat cataataacc ttttttggaa agttcaatga
     2941 aattttgaac cgtcttcggt gctagttcag ggaataattg aacagtaatg tcaccacggt
     3001 ttgttttgat aacagctttt ggtccttttt cactttttaa atctagttgt gggaatgcca
     3061 tattgtttcc tcctaatatt tgcatacttc ataataataa taacataata cctaacctct
     3121 cgtaaaaata ctagatttgt tttcttttat ttttcatcac tagatacatt ctataactta
     3181 ccgtttcgat ttcgaattca aacatttttg agaagcattc tcaattaaat tctgctatag
     3241 tgtgaaaagg gaggaatttt tatggaaatc tatgacatta caatcatcgg tgcaggtcct
     3301 gtagggatgt ttgccgcttt ttacgcagga atgcgccaag ctaaaacaaa aatcattgac
     3361 agtctacctc aacttggtgg tcaattagca actttgtatc ccgaaaaata tatttacgat
     3421 attcctgggt atccagcaat aaaagcaagt gaattaatcg atcaattaga aaaacaatta
     3481 actactttta accatacatt ccatttaaaa gaagaagttc tctcgcttac tcgtgaagac
     3541 gaagttatcg agattactac aaataaaggc atccattatt ccaaagcagt cattttagct
     3601 ttaggaaatg gttcattcca gccaagaaaa ttgaatttag ataatgctga atcatttgaa
     3661 aaccacggat tggattattt cgtgaatgat ttgatgaagt atgctggaaa aaaagtcgct
     3721 attgctggag gtggtgattc tgctatcgat tggacgttga tgttagagcc tattgcaagc
     3781 gaagtttact tgattcatcg tcgcccggaa tttcgtgcgc acgaacatag tgtatcacgc
     3841 ttgaaatctt catctgtcaa cttgcttact ccctatctca tcgatggatt atcaggcaat
     3901 aatggggaac tgacagatat ccggttaaaa aaagtgaaaa gtgacgagac gattgatttg
     3961 atgattgatt ctctgatcgt caattatgga ttctcttcta atttagaaca tctttcttct
     4021 tggggacttg atagtaccag aaatgccatt actgtaaaat cagatatgtc tacttctatt
     4081 ccaggcgttt atgcagctgg tgacatctgt acctatgaag gaaaagtgaa actgattgct
     4141 actgggctcg gtgaagcacc tactgcagtg aacaacgctt tacactatat taatccaaag
     4201 gaacgaactc agcctggtca cagcacaagc ttatacgata aaaatcttcg tcctagttga
     4261 ttttttatat ctctttgcat ttcaagttaa acccgaaagc agtttcttgc atttttttca
     4321 tgttccgtta acatgaaata gagaaagaaa aaataaaggg ggagcaataa tatggtcgtc
     4381 aaaactgaga ctttagaaga aaaggcaaga gaactattga aggaacgtgg cgtaactgta
     4441 gaagatatcg cacaacttgt catgttttta caaaaagact atatttctga tttgactttg
     4501 gcggattgta ctgaaagtgt caacagcgtg ctaacaaagc gtgaagtaca taacgccatt
     4561 atcactggaa tccaattaga tatattagca gaaaagaaaa aattgatgag tccgttgcaa
     4621 catattatcg aagatgacga aggcctttac ggtatcgatg agatcctagc cttgtcaatc
     4681 gtgaatgttt acggtacgat cggctttacg aattatggtt atatcgacaa ggtgaaacct
     4741 ggtattttaa aagaactaaa cagccacgat ggcgttcatg tccatacctt cttagatgat
     4801 atcgtcggag cgatcgccgc tgctgctgcc agtcgattag cacacaaaga accagaaaaa
     4861 agaagtcgcc ttactcaata attcaacaaa aaaagaaaga aaacgtctat ttagacgagt
     4921 tctttctttt ttgtttgttt caagctattt tttcctaaga aaagaaaaac agcaaaaaat
     4981 aattctaata atacaaaaaa taagataaag ctatgcatga aaaattcttc tggaagtaca
     5041 ttgatgattg gatcagtaac gggatcgaat aaccagtcat cgttgttgaa aaacgtttca
     5101 tgaaaaagga taaaaaagcg atcaaatcct atcatcatga aaaaaccgaa aacaactggc
     5161 agtaacatgc cgatttgaaa cggacgaatc aaccgccaaa gccgatcatt cttttgcaaa
     5221 tatttgataa acaaaatact cgggatcaaa gtgatgaaaa acacagcata atccactaaa
     5281 aacaaaattt tcacttcata aaagtgatgg gctccgctag cagaaactgg gaaatctggc
     5341 aagctgagga tggttttgaa gggattattg agataattca ttaggtgatc aaaatttttc
     5401 agtaacgtct cttgatctaa agaagtataa tccaaaattt gcaaatgtcc gatatcaaat
     5461 acgtatagtg gtcgaaagtt gatcgtcaca ccaactgcca gactgatcag tgtcagaaat
     5521 aggctgaaca atccagcata ttccatccac tgccatctcg aatttttcat attagaagct
     5581 ccattcatcc agtgattgtt tcacataggt agctgggata ggcaacccag gtacatcttc
     5641 ttcttttgta aaacctgata aaacgagcaa agtatctata ttattgcgta tcccagcttg
     5701 gatatctgtt tcatagttat ctccaaccat gattacttct tctttttgca gccctaatac
     5761 ttcaacggct ttatccataa tgatggcttc tggttttccg atgtagatcg gcggcgtttg
     5821 ggttgctgtc tctacaaaag agatgactga ccctgctcca ggaagaaggc cgcgttcagt
     5881 aggtatattc ttatctgggt tcgttccaat gaaagtagca cctttttgaa tagcaagtgt
     5941 tgctttaact actttttcat aggtcagata attatctaag cctaccacta catagtcagg
     6001 agcctcttct tcccatgtaa atccggcagc taaaatcaag tcaatcaatc ctgcttctcc
     6061 gataacatag acttttttcc cttttccatc tgctttcata aaatcgattg ttgccaaagt
     6121 agctgtgtag actgtctctg gagctacatg aatgtcaaac tcatctgcta gacgattagc
     6181 tactgtttct ggtgacttag tcgtgttatt ggtaacaaac aagaaaggta attttctttt
     6241 ttgcaattct tccacaaatc gtttaccggc cggtatgggt tgtgtcccac gataaatcgt
     6301 tccgtctaaa tcaattaaat atcctttata gtccattcat ccactactct tccctttttc
     6361 tgatcgtaaa tgctgttttt gttttttcta cttgtttagg ttgttcttgt ttttttactt
     6421 tttcagaggt ttttgctgac tctgtttttc gatttttaat aaccggtttt ttgcgattag
     6481 atgcgactgg tccttttttc tcatcgatat acgcacgatt atggtttttt ttcttttttc
     6541 ggcgctgagt tgtcttttct tttttaccgc cgattctctc gatgacaaaa tacgcacaac
     6601 caaaattgca atattcgtaa agatagtctt ccaaggaatc gattcgctga tctggatgcg
     6661 cccgtcgatt atccgcttgg aaaaatcctt ttaggcgtag ttgttcataa ccccaatctc
     6721 ctacgatata atcatatcgg gctagtacat cactgtaacg ttctccaagc ttttctgcat
     6781 taaatccttc acgataatcg gacacaagcc gataatgtct ttcacctatc aaaaaattac
     6841 tgtcatctaa cagtgtcact agctcgcctt tgactttttc tgctttaggc tcctcaacga
     6901 tttcttccaa agcagctgtc agttcttcgg tgactgttgt ttcctgttca gctgatgatt
     6961 ttttcttagc aatttctgtt ttatgctcaa cgttctgctg ttcattttgt tttgcagaca
     7021 tgcgttcacc acctatcttt tttattatac cttgttttta tttgattggg tagtgagctt
     7081 tcaagtaatc tcctaccaca cttcggataa attcagggaa caaaaattca ttttttccag
     7141 caatctcaat cgttggaaaa aatggaacaa acatcaaatg atctacggta gtaaaagtat
     7201 aatttttatt cagatcgatt ggcttgttct tccaaaagac ttgatggtta atagaatcat
     7261 atgtgattcc attatagacc atctgcccaa aaatcttacc gcgaaatccc attcccttca
     7321 taggatagtt acgtaaaaag ttacgatttt tttctatttc taaaactaag cggatcagat
     7381 catttccacc caatgtgaca tttaacaagt gcatcggatg cggcaacgca gtgtgaagct
     7441 gatcctgatt gacccgccct tttggaattt cagttaaaaa cagaccactg tttaatattg
     7501 ctgcttccgt ctttcctcgt tctttcaccg cctcaaaggc tacttggata aacgaatgct
     7561 cattgaagat atctagactc agagaaaatg gcaaatctgc tacttctttt tcttttaata
     7621 attcatgccc acgttctaga taactattta tttcttgttc atcttctgta aaagctgtca
     7681 ttgtttcggt agggatggct ttggctgaat gcttaaggat ttttttctct tcatcgatgg
     7741 ttaaatggac ttcaccgaca tactgcccgt actttccagc ggctgctatt tggacatcat
     7801 tgattacgtg accttctctg aataaatgat gggtgtgcga tcctaaaata acatcaattg
     7861 ctggtatttc ttttgcaatt tggaaatcat cctgaatccc gagatgactc atcaaaacta
     7921 aaacatccac ttgtgaacgt aaatcttcga ctaattctgg caaaatatca taaggattcc
     7981 gtatgtccca gccattcgga ttataagtaa gcggaaatgg agcagtaaat gcaattagtc
     8041 cgattttcgt ctggtgtttc gttgtaatga ttttatatgg tttcgtccat gccggtggct
     8101 ggagcgtatt tttatcgaat aagttatcca ggatgatatc aaactcagca tgatcgtata
     8161 aatgattcaa ctcttctttg gaatttccta cgccttcatt gtttcctatc gtcgctgcat
     8221 cataaccaac ttcattcatc agcaagatat tttcctgtcc attcgttgct tctgacaatg
     8281 gatgccaacg atccacaaaa tcacctagat ctactgcaat cgttgtcgtt ttttctttct
     8341 tttcgttttc tcgtttacgt tgatccaaaa accttctgat ccttggccaa ttttctaaat
     8401 gagaatgcag atcattcgta tgtaaaatca ctattttttc catgtaccct ctcgcctctc
     8461 tttatcttta tcataccata ggcagctttt tcttccattg aaacaagctc tatttcagag
     8521 tttttcctgc cttgaagcaa attgtataag tacaatattc gataaatcag tctggctaaa
     8581 aaatcaggaa cattcatttt tacagtacta taaaaataaa aaaaacagac ccgaaagtct
     8641 gttgtcgttt cattataatc agacacatgg cggaacctgc ttagtgctgc ttccttccag
     8701 acctgacacg cttcacaagc ccaccattgt cctagccttt cggcaaagaa tagtatacct
     8761 tatttttcat tcgttgacca gtcttttttt aatttctttt ttcgctggcg gagctttcgg
     8821 aaaaagtcag tcaaaatcat cccacattcg tcttctaaaa tgccaccttc tacatatgct
     8881 tggtggttaa accgctcgtc ctccaaaaga ttcatcaagg taccggctgt tccgcctttt
     8941 ggatcatacg cgccgaaata aacttctgct acacgagaca aaatcatcgc gccgctgcac
     9001 ataggacaag gctctaaggt aacaaaaagt gcagcatctt ccaagcgcca gcttcccact
     9061 ttttcacagg cttccttgat tgcaagcatt tctgcatgtg tagtagcatc ttgcgtggtt
     9121 tctctaaggt tatatcctct tccaatcact tttccatcca atacaaccac tgctccaatc
     9181 ggaacttcat gaagcatttc tgctttcttt gcttctgcca atgcaaggcg catccatttt
     9241 tctttttcta ctgcatcaag atttgtattt accaattacc cttccctgcc tttgtttttt
     9301 cttgttttac tttagcatat gataaaaagg ctgtaactaa cttttttttc tatggtaaaa
     9361 taaatacaag aggtgaacaa gatgaaaatg gataaattac ttgctcttta cccacaagca
     9421 cagaaaaaaa actctccagc atcagaagcg gatatccttt cttttgcaat tgatgatgct
     9481 tttatctgga tcaaacaaga ttcattatct caacaagaaa ccagcctttt gaaagcgtta
     9541 ttccctgtaa tcaacgatca aaaaaaacac ccatggtatc gctatctttt taaagatgct
     9601 tcttgtgttc aagaaaaaag ctttcgtttg atccagcttc acgtcgaatc aaaaggagaa
     9661 ttcttaaaaa aagaatggca agatactatt ttggaattat ttcgctattc tgctgatttc
     9721 tttttttata cagacacaga tgcgctctta gtcgaacaaa taaaccgtga ttatcttgat
     9781 gctactgaaa tcaacggtat ttttctatct ttagatgctg attttgatac gacaacgtct
     9841 gtattcgttg gttctttcca ttcacctggc accgacctcg tccctttgtt tgctgaagaa
     9901 agaaaaattt ttttaacaga gcaaaacaat ctttatactc acagccgtac tttttctatg
     9961 caagatgtgg ctttacatta ctatacaaaa gatactgtaa aagatagtaa gttgatcaaa
    10021 agttacatag aactgatcaa acaaaatgaa atggaagtga tcattcaaga attatggaat
    10081 gaattaggca atgtcagctc tgctgcaaaa acactgtttc ttcatcgtaa tactctaaaa
    10141 tatcgaatcg aaaaatttca agaacagtct ggtttcaatc taaaaaaagc caacgattta
    10201 ctgttttgct atcttttatt gcttcatgaa cataattaaa gatagattaa aggggttgtg
    10261 acaataattt tgtcacaacc ccttatttcg aatgaatagt gttcaaaagc tagtttctct
    10321 tacatcattt tttcaacaaa atgcattagc cggtctttta catcttcggt tgaatctttg
    10381 attgaatcac cataaacaga gattttcttt tttcctgatt tgatttttgc taacattccc
    10441 atcaaagaat ccaagtcact atctgaaaga ccatctacga tttctagtaa tttttcattt
    10501 ccatcaaatt tgtcatagac gaattttttt actttcgtcc gagtagtcat atgatggatc
    10561 ttttccatta cttttcccga tacaacgact gctgtcgcta cactggcagc ggctgctacg
    10621 cttaatccga ttgttacttt tgttgatgtt ttcataagta tcagctcctt tttatctatc
    10681 tccatcatag catagacaaa aaaataaacg aactaatcca ctcagtgaaa tctcaatcct
    10741 ttagggaaaa agttctttac tgttcctttt acttgtttcc caaatccgac aggtatctta
    10801 tctaatacta gcaaaaccca tccttgattc ccatcacgtt gaaatgtttc tcctgctgtg
    10861 taagattgcc attccttttg agtgatcggc aagcaaggta tattctctat cttttttgta
    10921 gcaagcgcta atgcatagct aggttcaaat cgattctttt tgaaatctcc taaatgcaaa
    10981 cctgtacgta cgacttttaa accatcgaga gaaggggcta actcaggaac ttcccaaaga
    11041 tggtcgttga acactaaaag tcttccagta gcttcataat gaaaatcatt tgaaaactcc
    11101 gtccagagtt tttcctgctc ttttgtcatt tgcactttac ttttttttcg tgtcttcttt
    11161 tctttatgca tctgattttg tccatgaaaa gtcagctttg caacaaaatg tccttcaccc
    11221 tgatctttat gaggccatat acgtattgtt ttttccaaac cagctacgct tccccattca
    11281 gaacgtcctg aactaacgga ttgagtcaaa ggaatctcct caatcgttac tggataattt
    11341 tctacaagcc agctaatgat ctcttcattt tcttctggtg caaacgtaca agtcgaataa
    11401 atcagttgtc ctttattttt tagcattttt attgctgaag acaaaatctc ttgctgacgt
    11461 ttttgacagt atagcggaga ttcttctgtc cactctttta ttgcatttgg atcttttcgg
    11521 aacattcctt ctcctgaaca aggagcatca accacgatcc gatcaaaaaa gcccgaaaaa
    11581 tgagggacaa gttctgcggg tgcatgattt gtcactattg cgttcgaaac tccccatcgt
    11641 tctatatttt ctgataaaat cttcgcacgt ttcggaaaga tctcattcgt cactaataac
    11701 cctttcccct tcatttgagc agctaattgg gttgatttac caccgggagc cgcacacaaa
    11761 tccaaaactt tttcaccggg ttttgctgca gcagctgttc caacgatcat tgcacttggt
    11821 tcttggctat attcataccc agcttgatgg aggaaagact ttccattgac agtgccaaga
    11881 aatccctcat tgctgtaggg agcgggttgt aattcttcgc tatggtaagt ttggaccatg
    11941 tccaatcctg ccggttttag tggattccac cgaaatcctt ttttcacgct tccttgttca
    12001 agcgcagaaa aaaaatcgga tgcttcttcg cctaaaagaa gacgatattt tttgataaat
    12061 tgctgtggta acgttgtcgc ttcctccttc attccaatgt ttcgttagac attttacaat
    12121 cttttttacg aaaaataaag gtggctgatc tactttttca tgttgctgcc taaggtgact
    12181 gttcctttaa gatattttcc aagtattcat ccgtcacctc gtttttatcc caaacagcgt
    12241 tcatcatttc aagaaaatct tgagaagaat catctgagcg caaacttccc aatgcattaa
    12301 gccataccaa ttcaagcgac ttttcttctt tgatcttcgt gtgacctttg acatatgaga
    12361 aaaatggccg cttaaatagc gaatccatgt ctaaatcaga ttttgcaatg atttcacgaa
    12421 tactttcatg gatagaaaaa aaatctttca ataagtagac aatgcttaaa acatcatcgg
    12481 aactgcattc agctaagtag ttttcaaaat aaacagtgat ttcttctaat atcttctctt
    12541 ttaacccgct gattgatttg aaatgtgtat aaatcgggta tgtagaacaa ccgcagaact
    12601 ctgctaactc tcttgcagta attgacttca ttccatattc ttccacgtat accatacttt
    12661 tgaacagcaa atattcttta ctataatttg ttactctccc catcattctt acctccgtct
    12721 cttttcatct acccataaat aaataacata aataattcta aaaatattat tgataattat
    12781 caaaacatat ttagttatat ccattaaaaa tcaatttttc cgccttaaaa acaagaatat
    12841 cttgacaaaa taccctagga aataaacaat agaataaaca aggtaacaaa aaaattaaat
    12901 aacgttatat aaaaaagaag gcgacactct gtgagaataa cacagactga aatgactttc
    12961 cactcacagt ttttgtctaa ctatatacaa aaacagaata aaaaggaggt atttatgcta
    13021 attgaagcaa gaaaaaaaga gttacttgtt ctacaaatgg tcattcaaaa aaataattgt
    13081 tctttacaag aattatccaa tgacttacat attccaaaac ggacaatcaa agaactgatc
    13141 cgaaaaataa atgtatcact caagcaattt ttattgattg atgaatttat ttattctaat
    13201 cataaaggag agatccaaac caaagaaacc gatcaaacta aaaagttagc tgttttttct
    13261 aagttaaaat tatgctacct aaaagaatcc aatcgtttta actatttgat gttactaatc
    13321 aattatccac aaactgctgt accaaaaaaa tatttacttg aacaacttta tatatcgccc
    13381 tcctatctcg aaaaactgac aaaacagctc aatcattcct taaaaaaatt ccatttacgg
    13441 atcctatctg taaaaaattg ctgtactctt gaaggaaatg agttatctat ccgtctctat
    13501 ctttactttt ttctttcaga tacatttgga ggtaacgatt ggccattttc ctcacttgaa
    13561 ttaaatgcac taaaaatgaa cagtacagat gagcaaatag aaaaaatttc aaaacaagaa
    13621 aaaaatatct acttgctgtt aactttattc atattacgaa acaaacaaca ggcatatttt
    13681 ccaaaaactt cttctgaaat cactgaattg atggacttat tgcagcatac acatgattat
    13741 tcagaatact ttacttcttt ttcagtgagt cgcccttgtg aagagacaga aacagagaga
    13801 gcctacttta attatttttt ctattgtttt tttccaaatc aaatctcttt tcgacaaaaa
    13861 caggagattg gttttttatt ttccacaagc aaaaacctta tttgtcgaca agtcggtcaa
    13921 ttgatgactt acttaactca acagtttcct ctcaaccatt tagaaaataa aataccaatt
    13981 tatttttatt ttcttgtgtt ggcagttgtt tttttcgaac tgttgggtga atccagcagt
    14041 agtttttcta cactttgctt tccagaagcc acgtccaatc attctagttc ttttatacag
    14101 gaaaagaaat taagagaaaa aatcgataca attttttcaa aaaatgggga aagaaaccaa
    14161 atcagtcaat accttagtca attgatccaa cacttactac aaacagaaac gaaagagcag
    14221 ttactcatcc atttgcagat aaccaaacag tttacaggaa attatcatct tcaaagccat
    14281 ttgcagcagt tatttcgcaa agatgctgtt aaaattaccg catattcaga acatgctgat
    14341 ttgatcatta cagatcagca tgagaaaatt tcagatgaaa cggagacttt ctatctggat
    14401 acaacgcaca atacagtgtt atttggcaag ttaataaaag cggttcatga actgtatatg
    14461 aaaaaaggtt atgagaacaa aacaaagagg atgtgacaaa attcttgtca catcctctta
    14521 tt
//
LOCUS       DACEEZ010000072        14391 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20313_2017
            SAMN12639966-rid9879223.denovo.072, whole genome shotgun sequence.
ACCESSION   DACEEZ010000072 DACEEZ010000000
VERSION     DACEEZ010000072.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639966
            Sequence Read Archive: SRR10023158
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 14391)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14391)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741683.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741683.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:44:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,016
            CDSs (total)                      :: 2,962
            Genes (coding)                    :: 2,889
            CDSs (with protein)               :: 2,889
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 36 of 73
            Pseudo Genes (incomplete)         :: 29 of 73
            Pseudo Genes (internal stop)      :: 18 of 73
            Pseudo Genes (multiple problems)  :: 8 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14391
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639966-rid9879223.denovo.072"
                     /isolate="E20313_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            4..552
                     /gene="hslV"
                     /locus_tag="IWO89_002161"
     CDS             4..552
                     /gene="hslV"
                     /locus_tag="IWO89_002161"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002357489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HslU--HslV peptidase proteolytic subunit"
                     /protein_id="HAQ0456882.1"
                     /translation="MVESPFHSTTICAVEKDGKFAMAGDGQVTMGESVVMKGTAKKVR
                     RIYNGEVVVGFAGSVADAFTLEEKFEGKLNEYNGNLQRAAVELAQEWRTQQSMQKLEA
                     MLIVMNDKEMLLVSGTGEVITPDDGILAIGSGGNFALAAARAMKNYAQNDMSAKEIAE
                     NALNIAADICIFTNHNIIVEEL"
     gene            566..1966
                     /gene="hslU"
                     /locus_tag="IWO89_002162"
     CDS             566..1966
                     /gene="hslU"
                     /locus_tag="IWO89_002162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease ATPase subunit HslU"
                     /protein_id="HAQ0456883.1"
                     /translation="MYELKQTPRQIVSELDEYIVGQHAAKKSVAVALRNRYRRLQLDE
                     KMQQDITPKNMLMIGPTGVGKTEIARRLAKIVNAPFVKVEATKFTEVGYVGRDVESMV
                     RDLVENAIQIVEKEQYSRVYTSAKKNAEKRLVKILVPGIKKEQKQSTNQFEQMMNLFN
                     ASQQNTAEPQEEVTEDIRINRRTVLEQLQKGLLDSREVTIEVEDQKKPAPMMNNGLEQ
                     MGIDLNETLGALTPKKKVERTVTVKEALELLIKEESAKLVKDADIHSEAIKLAEESGI
                     IFIDEIDKITSKSQQSGEVSREGVQRDILPIVEGSQVNTKYGIIQTDHVLFIASGAFH
                     LSKPSDLIPELQGRFPIRVELDDLTAEDFVKILTEPNNALVKQYVALIGTENVSVTFT
                     KEAIERIAQIAFDVNRDTDNIGARRLHTILEKLLEDLLFEAPDMQMGEITITEAYVDD
                     KLGSIAANEDLSRYIL"
     gene            1984..2775
                     /gene="codY"
                     /locus_tag="IWO89_002163"
     CDS             1984..2775
                     /gene="codY"
                     /locus_tag="IWO89_002163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-sensing pleiotropic transcriptional
                     regulator CodY"
                     /protein_id="HAQ0456884.1"
                     /translation="MTDLLTKTRKINTLLQQKNTFDMQADLPYDKMAVTLGGILDSNT
                     YIIGSDGVLLGYNERHDVNNERIKTMFVEKQFPESYTDTVAQLMKTEANIPITSDITA
                     FPVESREKYPFGLTTVVPIFGAGERLGTIILSRMEQSFADDDLVLAEYGATVVGMQIL
                     YQKSRSIEADVRSATAVQMAINTLSYSELKAVQAIFEALDGDEGRLTASNIADSIGIT
                     RSVIVNALRKLESAGIIESRSLGMKGTYLKVLNSRFKDELAKHSY"
     gene            2857..3732
                     /locus_tag="IWO89_002164"
     CDS             2857..3732
                     /locus_tag="IWO89_002164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose 1-epimerase family protein"
                     /protein_id="HAQ0456885.1"
                     /translation="MTVIIQNDQLIAEIAEHGAELISLKSKEHGLEYIWQGDPAYWGR
                     HAPVLFPFVGRLKDDQYVYQGKTYQMGQHGFARDMDFEVIEHGQEMASFILRSTPETL
                     EKYPFDFELVISYELGGDGITVHYQVANTGEKEMFFSIGGHPAFNVPLEEGLSFEDFY
                     LAFSPLKSRLRLPLKGPFIDMDQKTLGQTNTNLALNRELFEQDALVFETRGLNAYAIR
                     SEKSSHSVTLSYKDMPYVGIWSPYPKEAPFVCIEPWCGIADTVNSSGNLMEKVGINQL
                     GAHEIFKTKYSITVK"
     gene            complement(3864..4502)
                     /gene="plsY"
                     /locus_tag="IWO89_002165"
     CDS             complement(3864..4502)
                     /gene="plsY"
                     /locus_tag="IWO89_002165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsY"
                     /protein_id="HAQ0456886.1"
                     /translation="MKLILLLILAYLLGSIPSGVWIGKLFFKKDIRQHGSGNTGTTNT
                     FRVLGKTAGIVVLLMDILKGTLATCLPMIFHLSGINPLWFGVCAILGHTFPIFAGFKG
                     GKAVATSAGMLLGFNPVFFVYSCIIFIVSLFCTSMVSLSSMISAVLITLSTIVLPYVA
                     PVILAKPNWLLTIIAFLVSSFIFYRHKDNIKRIRNHTESRIPFGLNSSKKKS"
     gene            4629..5063
                     /locus_tag="IWO89_002166"
     CDS             4629..5063
                     /locus_tag="IWO89_002166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HAQ0456887.1"
                     /translation="MSVKNPTQETIFNYLEEAKNIAVVGLSNKPERTSYMVAQVLQDN
                     GYKIIPVNPLLAGEEILGEKVYANLTEIDDPIDIVDVFRRSEFLPEIAGDFLKTDAKV
                     FWAQLGIENKEAAEMLRKAGRTDIVMDRCIKIELGKLKENQK"
     gene            5229..7274
                     /gene="parE"
                     /locus_tag="IWO89_002167"
     CDS             5229..7274
                     /gene="parE"
                     /locus_tag="IWO89_002167"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit B"
                     /protein_id="HAQ0456888.1"
                     /translation="MAKKVNNEYNDSSIQVLEGLEAVRKRPGMYIGSTDSRGLHHLVY
                     EIVDNAVDEALSGYGSEIDVTIHEDNSITVADSGRGMPVGMHASGIPTVEVIFTVLHA
                     GGKFGQGGYKTSGGLHGVGASVVNALSKWLIVTIVRDGVEYQQKFKNGGKPDGTLKKI
                     GKTKKANGTTVHFLPDDTIFSTTKFSYEILAERLRESAFLLKGVKISLSDLRGEEPVK
                     EIFHYEEGIKEFVDYLNEEKDTLTPVVYFSGEKEGIEVEVAYQYNDGYSENVLSFVNN
                     VRTKDGGTHEAGMKAAMTKSYNEYARKVGLLKERDKNLEGSDFREGLAAVLSIRVPEN
                     LLQFEGQTKEKLGTPVARTVVDNVISEQMGFYLQENSEMSQMLVRKAIKAREAREAAR
                     KAREESRNGKKRKKGESLLSGKLTPAQSRNPKKNELYLVEGDSAGGSAKQGRDRKFQA
                     ILPLRGKVINTEKAKMQDILKNEEINTMIYTIGAGVGPEFSIEDCNYDKVIIMTDADT
                     DGAHIQVLLLTFFYRYMKPLIEAGKVYIALPPLYKVSKGQGKKQVIEYAWTDDELAAM
                     IKKVGKGYMLQRYKGLGEMNAEQLWETTMDPTSRTLIRVRIDDAAQAERRVTTLMGDK
                     VEPRRKWIENHVQFTLEEDGSILDKKEDTEISPSVSNDLLDEERADKNENNQLFEVE"
     gene            7286..9736
                     /gene="parC"
                     /locus_tag="IWO89_002168"
     CDS             7286..9736
                     /gene="parC"
                     /locus_tag="IWO89_002168"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit A"
                     /protein_id="HAQ0456889.1"
                     /translation="MENRQEVQELTLEEVMGDRFGRYSKYIIQERALPDIRDGLKPVQ
                     RRILFAMNKDGNTYDKGFRKSAKSVGNIMGNYHPHGDISIYEAMVRMSQDWKLREVLI
                     EMHGNNGSMDGDPPAAMRYTEARLSKLSGEMLADIEKETVDLVWNFDDTEKEPTVLPA
                     RYPNLLVNGSTGISAGYATEIPTHNLAEVIDGTIYMIDHPQASLEKLMEYIPGPDFPT
                     GGILQGKDEIKKAYETGRGKVILRSKTKIEAMKGNKQQIVITEIPYEVNKATLVKKMD
                     EIRLNKKIDGIAEVRDESDRTGLQIVVELKKDANAQGILNYLFKNTELQINYNFNMVA
                     IDHMTPHQVGLKDILRSYIEHRKQVITKRSQFDLAKAQKRQHIVEGLMKALSILDEVI
                     ATIRESKDKKDAKKNLVDVFQFTEEQAEAIVTLQLYRLTNTDITELQKESESLIAQIT
                     ELNKILSNDKELFSVMKKELREVKKNYSSARLTTIEDEIEEIKIDTQVLVAQEDVIVS
                     VTREGYVKRTSLRSYSASKPEEIGMREGDYLLYSGELSTLDHVLLITNKANVIYRPVH
                     ELPDLKWKDAGEHISQTISNLSVDESILAVFPYQKIQAEKTFVFISKNGLIKQTRMTE
                     FEPWRTYKSRPLSCMKLKAEEDELIAVYLKEDQPGLDVFLVTHQGMGLRYPLTDVPVV
                     GAKAAGVKSMNLKDGDRIINGLLVLAEGDTPIVIVTQRGAVKRMLAQEISQTSRAKRG
                     VTVMRELKKNPHRIIYMSEGRSKEITLMNQKGQQLTIDPTDFPIGDRTSNGSFAMDEK
                     KGGEVIEVIDAPEIFIES"
     gene            10013..12244
                     /gene="pflB"
                     /locus_tag="IWO89_002169"
     CDS             10013..12244
                     /gene="pflB"
                     /locus_tag="IWO89_002169"
                     /EC_number="2.3.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate C-acetyltransferase"
                     /protein_id="HAQ0456890.1"
                     /translation="MEQWNGFKGTKWKEGVDTRDFIQNNYTEYRGDDSFLEGPTEATE
                     KLWDELQKLNDIQFERNGVYDMDTEIVSTITSHDAGYLDKDLEKIVGLQTDKPLKQAF
                     MPFGGINMANNALKSNGYTPNDELTHIFTDWRKTHNQGVFDAYTAEMRAARKNKIITG
                     LPDAYGRGRIIGDYRRVALYGIDYLMEQKKKDHDMTGYHTMSEEVIRLREEITEQYRA
                     LAQMKEMAAKYGYDISKPAANAKEAVQWLYFGYLAAIKSQNGAAMSIGRISAFLDIYI
                     QRDLDNGVITEKEAQELIDHLVMKLRMVKFARTPEYNQLFSGFPIWATLSIAGMGLDG
                     RSLVTKNDFRLLHTLTNMGPSPEPNLTVLYSEHLPEGFRTYASRIAIESSSIQFENDN
                     QLREQWGSDDCAIACCVSGTVVGKDMQFFGARANLAKTLLYAINGGVDEVTGAQVGPE
                     YRPITSEILDFEEVKHNFMNMMDWLAELYVNTLNIIHYMHDKYSYEAAQLALMDSDLQ
                     RTFATGIAGISHAADSLSAIKYAKVKAIRNEKGIAVDFEVDGDFPKYGNDDDRADELV
                     SWILEYFMGQIKRHKTYRNSKPTTSLLTITSNVVYGKNTGNTPDGREAGKPLAPGANP
                     SYNAEQSGLLASLNSTAKMNYGCALDGISNTQTINPSGLGKEQDTRIENLRNVLDGYF
                     SKGAYHLNVNVFSNDLLRDAMENPMKYPNLTIRVSGYAVKFRDLTREQQLDVLMRTSH
                     DRM"
     gene            12488..13249
                     /gene="pflA"
                     /locus_tag="IWO89_002170"
     CDS             12488..13249
                     /gene="pflA"
                     /locus_tag="IWO89_002170"
                     /EC_number="1.97.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate formate lyase-activating protein"
                     /protein_id="HAQ0456891.1"
                     /translation="MEEKTIGYVHSIETFGSVDGPGLRFVVFMQGCRMRCQFCHNPDT
                     WNIGGGKEYTADELLDKAERFRPYWGDKGGITVSGGEPLLQIDFLIELFKKAKERKMH
                     TTLDTCGKPFTYEEPFFSRFQELMKYTDLLLFDIKHIDNEEHKKLTHWDNENILEMAQ
                     YLSKINKPVWIRHVLVPERSDYDEYLIRLDNFIQTLSNVDRVEILPYHTMGKYKWETL
                     GLKYPLEGIEPPTKERVENAKRLLHTEEYTKYLTR"
     gene            13334..14260
                     /locus_tag="IWO89_002171"
     CDS             13334..14260
                     /locus_tag="IWO89_002171"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese-dependent inorganic pyrophosphatase"
                     /protein_id="HAQ0456892.1"
                     /translation="MSKILVFGHQNPDTDAIGAAIAFSHLQNELGKETEAVALGTPNE
                     ETQYALDHFQLSAPRVVESAKAENADVMLVDHNEFQQSISDISEVNILAVVDHHRIAN
                     FETANPLYYRAEPVGCTSTIILKLYKENQVTIPKEIAGIMLSAIISDTLLFKSPTCTP
                     EDEAAAKELAAIAEVDLNTYGLEMLKAGTNLSDKSAAVLLDLDAKSFPMGSSNVRIAQ
                     VNTVDLNEVMDRQAELEAAMEDEMKANGYDLFVLVVTNILDSDSELLVAGKGKEKVEE
                     AFQTTLTNNRALLKGVVSRKKQVVPQLTAVFS"
BASE COUNT         5049 a         2494 c         3124 g         3724 t
ORIGIN      
        1 aacatggtcg aatcaccatt tcattcaacc actatctgtg ccgtcgaaaa agacgggaaa
       61 tttgctatgg ctggagatgg ccaagtaaca atgggtgaat ctgttgtcat gaaaggcact
      121 gcaaagaaag tacgacgtat ctacaatgga gaagttgttg taggtttcgc cggaagtgtt
      181 gcagatgcgt ttactttaga agaaaaattt gaaggcaaac taaacgaata taacgggaat
      241 ctacaacgcg cagctgtcga attagctcaa gaatggcgga cacagcaatc tatgcaaaaa
      301 cttgaagcga tgctgatcgt aatgaatgat aaagaaatgc ttcttgtttc aggtacagga
      361 gaagttatca caccagatga tggtatctta gctatcggat caggagggaa ttttgcgttg
      421 gctgcagccc gagcaatgaa aaactatgct caaaacgaca tgagtgcgaa agaaattgca
      481 gaaaatgcgt taaatattgc cgcagatatc tgtatcttta ctaatcataa tattatcgta
      541 gaagaactgt aagaggtgag tagatatgta tgaattaaaa caaacaccaa gacaaatcgt
      601 aagtgaatta gatgaatata tcgtaggaca gcacgcagca aaaaaatctg ttgccgttgc
      661 tttgcgtaac cgctatcgcc gtttgcagtt agatgaaaaa atgcaacaag atattacacc
      721 aaaaaatatg ctgatgatcg gacctacagg ggtcggtaaa acagaaatcg cacgacgttt
      781 ggcaaaaatc gtcaatgcac catttgtcaa agtcgaagca acaaaattta ctgaagtcgg
      841 ttatgtcgga agagatgtag aatcaatggt tcgggattta gtagaaaatg cgatccaaat
      901 cgtagaaaaa gagcagtaca gccgtgtata cacaagtgct aaaaaaaatg cagaaaaacg
      961 tttggtaaaa atcttggtac ctggtatcaa aaaagaacag aaacaatcca ctaatcaatt
     1021 tgaacaaatg atgaatcttt ttaatgctag tcagcaaaat accgctgaac cgcaagaaga
     1081 agtgacagaa gatattcgaa tcaatcgccg tacagtcttg gaacaactac aaaagggttt
     1141 actggatagt cgtgaagtaa ccattgaagt tgaagaccaa aagaaacctg caccaatgat
     1201 gaacaatgga ttggagcaaa tggggatcga cctaaatgaa actctaggtg ctttgacacc
     1261 gaagaaaaaa gtggaacgga cggtaacagt caaagaagct ttagagttat tgatcaagga
     1321 agaatcagca aaattagtca aggatgctga tatccatagt gaggcaatca aattagcgga
     1381 agaaagtgga attattttta tcgatgaaat cgataagatc acttctaaat cacagcaatc
     1441 tggagaagta tccagagaag gcgtccaaag agacatattg ccaattgtcg aaggttcaca
     1501 agtaaacaca aaatacggga tcatccaaac agatcacgtc ctattcattg cttcaggagc
     1561 tttccatttg tctaaaccaa gtgatctaat tccagaactt caaggacgct tcccgatacg
     1621 ggtagaatta gatgatttga cagctgaaga ttttgtgaag attctgaccg aaccaaataa
     1681 tgctttagta aaacaatacg tcgcgttgat cggtacagaa aatgtgtcag ttacgtttac
     1741 aaaagaagca attgaacgga tcgctcaaat cgctttcgat gtcaaccgtg acacagataa
     1801 tatcggcgca cgcagactgc ataccatttt ggaaaaacta ctagaagatc tattatttga
     1861 agcaccagat atgcaaatgg gagaaataac gatcacagaa gcatatgtgg atgacaaact
     1921 tggctcaatt gcggcaaatg aagatttaag tcgctatatt ttataaaaaa tcggaggacg
     1981 acaatgacag acttgctaac gaaaacccgc aagataaaca cacttttgca gcaaaagaat
     2041 acttttgaca tgcaagcaga tttgccttac gataaaatgg ctgtcacact aggtggtatt
     2101 cttgacagca acacctatat cataggaagt gacggagtat tactaggtta taatgaacgt
     2161 cacgatgtga acaatgaacg aatcaaaaca atgtttgttg agaaacaatt tcctgaatct
     2221 tatactgata cagtggcaca gttgatgaaa actgaagcta atatcccaat tactagtgat
     2281 attactgcat ttccggtaga gtcaagagag aaatatccat ttggtctgac gactgtcgtt
     2341 ccgatctttg gagctggcga acgtttgggt acgattatct tatctcgtat ggaacaatcg
     2401 tttgcagatg acgatctagt cttagcagaa tatggagcta cggttgtcgg tatgcagatc
     2461 ctttatcaga agtctcgaag catcgaagca gatgtacgga gtgcaacggc tgtccaaatg
     2521 gcaatcaaca cattatctta tagcgaattg aaggctgtac aagctatctt tgaagcattg
     2581 gatggagatg aaggtcgtct gactgcttct aatattgccg atagtatcgg catcactcgt
     2641 tctgtgatcg tcaatgcttt acgaaaattg gaatcagctg gaatcattga atctcgttcg
     2701 ttaggcatga agggaactta tttaaaagta ttgaattccc gttttaaaga cgagttagca
     2761 aaacacagtt attagaagta aggggactgg ggcagataga aatgtttgtt ccagccttcc
     2821 cattttaaaa agtaagtcga agttggagga atggcaatga ctgttattat tcaaaatgac
     2881 cagctgatcg ccgaaatcgc tgaacacggt gcggaattga tcagtctgaa atcaaaagaa
     2941 catggtttag agtatatctg gcaaggtgat ccagcgtatt ggggcagaca tgcaccggtt
     3001 ttattccctt ttgttggacg tttaaaagat gatcagtatg tttatcaagg gaaaacatat
     3061 caaatggggc aacacggatt tgctcgggat atggattttg aagtcattga acatggacaa
     3121 gaaatggcta gtttcatctt aaggagtacg ccggagacat tagaaaagta tccatttgat
     3181 tttgaattag tgatcagcta tgaattagga ggagacggca tcacggttca ttatcaagtg
     3241 gcaaataccg gtgaaaaaga aatgttcttt tctatcggtg gacatcctgc tttcaatgta
     3301 cctcttgaag aaggattgtc atttgaagat ttctatctgg cattttctcc attgaaatca
     3361 agattacgcc taccattgaa aggcccattc atcgatatgg atcaaaagac gttaggtcag
     3421 acaaatacga atctggcttt gaatcgtgaa ctttttgagc aagatgcctt ggtttttgaa
     3481 acaaggggtc taaatgcgta cgccattcga agtgaaaaat cttcccatag cgtaacttta
     3541 agctataaag atatgcctta tgtagggatc tggtctcctt atccaaaaga agcgcctttc
     3601 gtttgtatcg aaccgtggtg cggaatcgct gacacggtga acagtagtgg aaacttgatg
     3661 gaaaaagtag ggatcaatca actaggtgcc catgaaatct tcaagacaaa atattccatc
     3721 acagtgaagt aagaggttgt gaccgagaag ttagcaggga acaccgttta ccaaggtgtg
     3781 taaaaaagct atcctgtaag taataaggga gtgtgacatc gatttgtcac actcccgttt
     3841 tatctctttg gaatgaaaaa gtatcaggat tttttcttgc tgctattcaa accaaacggg
     3901 attcgacttt cagtatggtt tctgatacgc ttgatattgt ctttatgccg ataaaagata
     3961 aaggaagaaa ctaaaaaggc aatgatcgtc agtaaccaat tgggcttagc caaaatcaca
     4021 ggagctacat aaggaaggac gatcgtagat aaagtgatca atactgcact gatcatactg
     4081 ctcaaactca ccatacttgt acaaaataaa ctaacgataa agatgataca agaataaaca
     4141 aaaaatacag gattaaatcc taaaagcatt cctgcacttg tcgcaacagc ttttcctcct
     4201 ttgaacccag caaaaatcgg aaatgtatgg ccaagaatgg cgcatacccc aaaccaaaga
     4261 gggttaatcc cagaaaggtg gaagatcatc ggcaaacaag tagccaatgt accttttaaa
     4321 atatccataa gcaggacaac aatcccggct gttttgccta atacacgaaa tgtattggtc
     4381 gttccagtat taccgctccc gtgttgtcgg atatcttttt tgaaaaacaa tttgccaatc
     4441 cagacaccgg aaggaataga acctaaaaga tatgctaaaa taagtaataa gatcaatttc
     4501 attttttacc ctctttcaag ttgaaaacta tcctttattt tagcatgaat aggtaacagg
     4561 aacaattgaa aaaatgaaag atacaagtat aatagatgga ataaaaagaa aagcaagggg
     4621 tggcagaaat gtctgtaaaa aatccaacac aagaaacgat tttcaactat ttggaagaag
     4681 caaaaaatat tgccgtcgta ggcttgagca ataaaccaga acgaacaagt tacatggttg
     4741 cacaggtatt gcaagacaat ggatataaaa tcataccagt aaatccatta ttggctggtg
     4801 aagaaattct aggcgagaaa gtctatgcga atttgacaga aatcgatgat ccaatcgata
     4861 tcgtagatgt atttagacgg agtgaatttt tacctgaaat agcaggtgat tttttgaaaa
     4921 ctgatgctaa agtgttctgg gcacaattag ggattgaaaa caaagaagca gctgagatgc
     4981 taagaaaagc aggacgtaca gatatcgtaa tggatcgttg tattaagatt gaacttggta
     5041 agttgaaaga aaaccaaaaa taaaaatgac cacaaagaag agatgatcga tagaggggaa
     5101 tgtcgaaaaa gcggttagga gataattaaa attgatttct cgtctaccct cttttttttc
     5161 acgacctttt ctagtatgat aatcgtggtg cggttttgcc gcaaagtatt attgaaggag
     5221 cgtttgcctt ggcaaaaaaa gtaaacaacg aatacaatga ctcgtccatc caagtattgg
     5281 aaggattaga ggcagttaga aaaagaccag ggatgtacat tggatccact gacagccgag
     5341 gcttgcatca tttagtctat gaaatcgtcg ataatgcagt tgacgaagca ttatcaggtt
     5401 atggcagtga gattgatgtg accatccatg aagacaatag tataactgtt gcagatagcg
     5461 gacgtgggat gcctgtagga atgcatgctt ccggaatacc aacagttgaa gtgattttta
     5521 ctgtgctgca tgctggcgga aagtttggtc aaggagggta caaaacttct ggtggactgc
     5581 acggtgtagg agccagtgtg gtaaatgcct tatcgaaatg gctaattgta acgatcgtta
     5641 gagatggcgt agagtatcag cagaaattta aaaacggcgg taaacctgac ggtactttga
     5701 aaaagatagg aaaaacaaaa aaagcaaacg ggacgactgt ccatttttta ccagatgata
     5761 caatattcag tacaacgaaa ttttcatatg aaattttagc cgaacggtta agagaatctg
     5821 cctttcttct aaaaggcgtt aaaatctctc tttctgatct tcgtggagaa gaaccagtca
     5881 aggaaatttt tcattacgaa gaaggaatca aagaatttgt tgattatctg aatgaagaaa
     5941 aagatacatt gacaccagtt gtttattttt caggagaaaa agaaggtatc gaagtagagg
     6001 tcgcttatca atacaatgac ggttattcag aaaatgtttt atcttttgtc aataacgttc
     6061 ggacaaaaga tgggggaacc catgaagctg gtatgaaagc ggcgatgacg aaatcttata
     6121 atgaatatgc gagaaaagtt ggtttgctga aagaacggga caagaatctg gaaggtagtg
     6181 atttcagaga agggttagct gctgtacttt ctatccgtgt acctgaaaat cttctgcaat
     6241 ttgaagggca aacaaaagaa aaacttggta cgccagttgc tcgtaccgta gtagataacg
     6301 tcatcagtga acaaatgggc ttttatttgc aggaaaatag cgaaatgagc cagatgttgg
     6361 tccgtaaagc aatcaaagcc agagaagcga gagaagcagc acgtaaagcc agagaagaaa
     6421 gccgaaatgg gaagaagcgt aaaaaaggcg aatctcttct ttcaggaaaa ttgacacccg
     6481 cacaatcccg aaaccctaag aaaaatgaat tgtatttagt cgaaggggat tctgctggcg
     6541 gatctgctaa acaaggaaga gacagaaagt tccaagcaat tctaccttta cgaggaaaag
     6601 tcatcaatac cgaaaaagct aaaatgcaag atatcttgaa aaatgaagaa atcaatacga
     6661 tgatctatac gattggtgca ggagtcggtc ctgagttttc tatcgaagac tgtaattacg
     6721 acaaagtaat catcatgacc gatgcggata cagacggcgc ccatattcaa gtattactgc
     6781 ttactttctt ttatcgttat atgaaaccgc tgattgaagc tgggaaagta tatatcgcgt
     6841 taccgccttt atataaagta tccaaaggac aaggaaagaa gcaagtcatt gaatatgcgt
     6901 ggacagatga cgaattggca gcaatgatta aaaaagtcgg caaaggatat atgctgcagc
     6961 gatataaagg acttggagag atgaatgcgg aacaattgtg ggaaacaacg atggatccga
     7021 cttcccgtac gttgatccgc gtccgtatcg atgatgctgc gcaagctgaa cgtcgagtaa
     7081 ccacactgat gggtgacaaa gtagaacctc gaagaaaatg gatcgaaaat cacgtgcaat
     7141 ttacgctcga agaagatggc agtatcttag ataaaaaaga ggatactgaa atctctcctt
     7201 cagtttctaa tgatttatta gatgaagaac gagcagataa aaatgaaaac aatcagttat
     7261 ttgaagtaga ataggaggtc acctgatgga aaatcgacaa gaagtacaag aattaacttt
     7321 agaagaagtg atgggtgacc gcttcggacg ttattccaaa tatattatcc aagaacgtgc
     7381 actgcctgat atccgtgatg gattgaagcc agtgcaacgc cggatattat ttgccatgaa
     7441 caaagatggc aatacgtatg acaaaggatt ccgtaaatca gcaaaatctg tcggaaatat
     7501 catggggaat tatcatcctc atggtgacat cagtatttat gaagcgatgg tccgcatgag
     7561 ccaagactgg aagttgcggg aagtactgat cgaaatgcac gggaataacg ggagtatgga
     7621 cggtgatcca cctgctgcga tgcgttatac tgaagccaga ttatccaagt taagtggaga
     7681 gatgctagca gatatcgaga aagaaacagt cgatctcgtg tggaattttg atgatacaga
     7741 aaaagaacca acagtccttc cagctcgata cccaaacctt ctcgtcaatg gatcgacagg
     7801 tatatctgct ggatatgcaa cagaaatccc gactcataac ttagctgaag tgatcgatgg
     7861 cacaatctat atgatcgatc atccacaagc gagccttgaa aaattgatgg aatatattcc
     7921 tggacctgac tttccaacag gagggatctt gcaaggaaaa gatgaaatca aaaaagctta
     7981 tgaaacagga cgaggaaaag ttattcttcg ttcgaaaaca aagatagagg caatgaaggg
     8041 taataaacag caaatcgtga tcacagaaat cccatatgaa gtaaataaag cgaccttggt
     8101 gaaaaaaatg gatgagatcc gtttgaataa gaaaatcgat ggaatagctg aagtacgaga
     8161 tgaaagtgat agaacaggtc tgcaaattgt cgtcgaattg aaaaaagacg ccaatgctca
     8221 aggaattttg aattatttgt tcaaaaacac tgagctacag atcaattaca atttcaatat
     8281 ggtcgctatc gaccatatga ctccgcatca agttggactg aaagacattt taagaagtta
     8341 tatcgagcat cgaaagcaag tcataacaaa acgtagtcaa ttcgatttag ccaaagctca
     8401 aaaaagacag catatcgtag aaggattgat gaaagcttta tcgattctgg atgaagtaat
     8461 cgcaacgatt cgtgaaagta aagacaaaaa agacgccaaa aagaatttag tcgacgtctt
     8521 tcaatttaca gaagaacaag cagaagcaat tgtcacattg caattatacc gtttgacgaa
     8581 cacggatatc acagagcttc aaaaggaatc cgaaagcttg attgcgcaaa tcacagagtt
     8641 gaataaaatc ctttcaaatg acaaagagct tttctctgtt atgaaaaaag aattgcgaga
     8701 agtgaaaaaa aattattctt ccgctcgtct aacgacaatc gaagacgaaa tcgaagaaat
     8761 caaaattgat acgcaagtct tagtggcaca agaagacgta attgtcagtg tgacgcgaga
     8821 aggctatgtg aagcgaacaa gccttcgttc ttatagtgct tccaaaccag aagaaatcgg
     8881 gatgcgagaa ggcgactact tgctatactc aggtgaattg agtacattgg accatgttct
     8941 tctgatcaca aataaagcaa atgtgatcta ccgccctgtc catgaactac ccgatttgaa
     9001 atggaaagat gctggtgagc acatttctca aacaatctcc aatctgtctg tagacgaatc
     9061 gattttagct gttttcccat atcaaaaaat tcaagcagag aaaacatttg tattcatcag
     9121 caagaatgga ttgatcaagc aaacacgaat gacagagttc gaaccatggc gcacctataa
     9181 gagccgtccg ctcagctgta tgaagctaaa ggcggaagaa gatgaactga ttgctgtgta
     9241 tctgaaagaa gatcagccag gcttagatgt tttcttagta acgcatcaag gaatgggttt
     9301 gcgctatcct ttaacagatg ttcccgttgt tggagcaaag gcagctggag tgaagtctat
     9361 gaatttgaaa gatggagata ggattatcaa cgggctgctt gtgttagcag aaggtgatac
     9421 gccgatcgta atcgttactc agcgaggagc tgttaaacgc atgcttgctc aagaaatcag
     9481 ccaaacttca cgtgcgaagc ggggagtgac ggtcatgcgt gagctgaaaa aaaatccgca
     9541 ccgtattatc tatatgtcgg aaggaagatc aaaagaaata accttgatga atcaaaaagg
     9601 gcagcagtta acgattgacc caactgattt ccctataggt gatcgaacat cgaatggatc
     9661 atttgcgatg gatgagaaaa aaggtggaga agtcatcgaa gttatcgatg ctcctgagat
     9721 tttcatcgag tcgtaactgt tgtagtattt taatgaagag acgccatctg tattaataca
     9781 ggtggcgtct ctttttcatt catttttaat aaatccttat acgtttgcaa aaaaagatga
     9841 aagtgttcac aatgcttaaa tgttggtatg tcaacgttga tttaaatata tgtaaataaa
     9901 acataaaaat tgttaaataa aaaacaagaa aacccttgca aagatagggt gctgtgttat
     9961 actaatcacg taaaaactgt tacataaaac agaaatttag gaggtccata caatggagca
    10021 atggaatgga tttaaaggta ccaaatggaa agaaggcgta gacactagag actttattca
    10081 aaataactac actgaatatc gcggtgatga cagctttttg gaaggcccaa cagaagcaac
    10141 agaaaaatta tgggatgaat tgcaaaagtt aaatgatatc caattcgagc gtaacggtgt
    10201 ctatgatatg gacacagaaa tcgtatcaac tattacttca cacgatgcag gctatttgga
    10261 taaagatttg gaaaaaatcg tcggtctaca gacagacaaa cctttgaaac aagcattcat
    10321 gccgttcggc ggaatcaata tggctaacaa tgcgctaaaa agcaatggct atacaccaaa
    10381 cgatgaattg acacatattt tcactgactg gcgcaaaaca cataatcaag gtgtatttga
    10441 cgcttatact gctgaaatgc gtgctgctcg taaaaacaaa atcattacag ggcttccaga
    10501 cgcttacggt cgcggacgta tcatcggtga ctatcgtcgt gtggcattgt atggaatcga
    10561 ctatttgatg gaacaaaaga aaaaagatca tgatatgact ggttatcata caatgagtga
    10621 agaagtgatt cgtttacgtg aagaaatcac tgaacaatat cgtgcattag cgcaaatgaa
    10681 agaaatggct gcgaaatatg gctatgatat ttctaaacca gctgcaaatg caaaagaagc
    10741 tgtccaatgg ctatacttcg gatatttagc agcaatcaaa tcacaaaatg gtgctgcgat
    10801 gtcaatcgga cgtatctcag cattccttga tatctatatc caacgtgatt tggataatgg
    10861 agtaatcaca gaaaaagaag cacaagaatt gatcgaccac ttagtaatga aattacgtat
    10921 ggtcaaattc gcgcgtacac ctgaatataa ccaattattc tcaggattcc ctatctgggc
    10981 tactttatcc attgcaggta tgggattaga tggtcgttca ttagttacaa agaatgattt
    11041 ccgtctgctt catacattaa caaatatggg accttcacca gaaccaaact tgactgtttt
    11101 gtattctgaa caccttccag aaggattcag aacatatgct agcagaatcg ctatcgaaag
    11161 ttcttcgatt caattcgaaa atgataatca gcttcgtgaa caatggggtt ctgatgattg
    11221 tgcgattgct tgctgtgtat caggaacagt tgtcggaaaa gacatgcaat tctttggcgc
    11281 tcgtgctaac ttggctaaaa cactattata tgccatcaat ggcggagtcg atgaagtgac
    11341 tggcgcccaa gttggaccag aatatcgtcc aatcacatca gaaatactag actttgaaga
    11401 agtgaaacat aacttcatga atatgatgga ttggttagca gaactatatg tcaatacgtt
    11461 gaacatcatt cattatatgc atgataaata ttcttatgaa gcagctcagc tggctttgat
    11521 ggattctgac ttgcaacgca catttgctac aggtattgct gggatctctc atgcggcaga
    11581 cagcttgtca gcgatcaaat atgcaaaagt aaaagcaatc cgtaatgaaa aaggaatagc
    11641 agttgatttt gaagtggatg gcgacttccc taaatacgga aatgatgatg accgtgcaga
    11701 tgaattagtt tcatggatct tggaatactt catgggacaa atcaaacgtc ataaaactta
    11761 ccgcaactca aaaccaacaa cttcattatt gacgattact tcaaatgttg tttacggtaa
    11821 aaatacagga aatactcctg atggccgtga agctggaaaa ccacttgcgc caggagcaaa
    11881 tccaagttac aatgcggaac aaagtgggtt attagctagc ctgaactcaa cagctaaaat
    11941 gaattacggc tgtgcattgg atggaatttc aaatacccaa acaatcaatc cttcaggtct
    12001 tggaaaagaa caagatacaa gaatcgaaaa cttgcgtaat gtcttagatg gatacttctc
    12061 aaaaggagct tatcatttga atgtcaatgt attcagtaat gatttattgc gtgatgcaat
    12121 ggaaaatcca atgaaatatc cgaacttgac gatccgggta tctggttatg cagttaaatt
    12181 ccgtgacttg acaagagaac aacaattaga tgtcttgatg agaacttcac acgacagaat
    12241 gtaaaaagtt cgtgaaaaaa gtttttcgac ttagcgtcga aaggacgttg aatagccatc
    12301 gcatcaaaat cgaataatca gtatgcttat ttgaaagaaa cgatttacag gggcaggact
    12361 tgtatgttcc gtccctgtaa attttacaca taacgaagcg gcaattgcga aaaattttgt
    12421 tcagaaagct tcgcttcgac agctaacttg tacttaaaat gaaatcaaag aaaagatgtg
    12481 aacttaaatg gaagaaaaaa caatcggcta tgttcattct atcgaaactt tcggttcagt
    12541 agacggacct ggactgcgct ttgtcgtgtt tatgcaagga tgccgcatgc gttgccaatt
    12601 ttgtcacaat ccagatactt ggaatattgg cggagggaaa gaatacacag cagatgaact
    12661 tttagataaa gcagaacgct tcagacctta ctggggagat aaaggtggta ttactgttag
    12721 tggtggagaa cctctattgc agatcgattt tttgatcgaa ctatttaaaa aagcaaaaga
    12781 gcggaagatg catactacat tggatacatg cggaaaacct tttacgtatg aagaaccttt
    12841 cttttcacgt tttcaagaat tgatgaagta tacagattta ctgttatttg acatcaaaca
    12901 catcgataat gaagagcata aaaaactaac gcattgggac aatgaaaata ttttagaaat
    12961 ggctcaatat ttgtcaaaaa tcaacaaacc ggtttggatt cgccatgtac tagttcctga
    13021 gcgaagtgat tatgatgaat atttgatccg actagacaac tttattcaaa ccttgtcaaa
    13081 tgtagacaga gtagagattc ttccttatca tacgatggga aaatacaagt gggaaacact
    13141 tggtttgaaa tatccattag aagggatcga accacctaca aaagaacgtg tcgaaaatgc
    13201 taaacgattg cttcatacag aagagtatac aaaatattta acgcgatagg tcgcatcgga
    13261 aagaagataa gtattgcttc tttccgtttt tttgctatta ttaaatagga gaaatcacaa
    13321 aggagcgaat gacatgtcaa aaattttagt ttttggtcac caaaatccag atacagatgc
    13381 aatcggggca gccattgcgt tttcacactt gcaaaatgaa ttaggtaaag aaacagaagc
    13441 agttgctcta ggtacaccta atgaagaaac acaatacgca ttagaccact ttcagctatc
    13501 tgcaccacga gtagtagagt cagctaaagc agaaaatgca gatgtcatgc ttgtggatca
    13561 taatgaattc caacaaagta tttcagatat cagcgaagta aatattttag ctgtagtaga
    13621 ccaccatcgt attgcaaact ttgaaacagc taatccattg tactatcgtg cagaaccagt
    13681 tggatgtaca agtacgatta ttttaaaact atataaagaa aaccaagtaa caattcctaa
    13741 agaaatcgca ggaatcatgc tctcagctat catttcagat acattgttgt tcaaatcacc
    13801 aacatgtacg cctgaagatg aagcagctgc aaaagaactt gcagcaatcg ctgaagtcga
    13861 tctaaacact tatggacttg aaatgttgaa agcaggaaca aatctaagtg ataaatcagc
    13921 tgcggttctt cttgatttgg atgcaaaaag tttcccaatg ggcagttcca acgttcgtat
    13981 cgctcaagtc aatacagtgg accttaatga agtgatggat cgacaagcag agctagaagc
    14041 agcaatggaa gatgaaatga aagcaaatgg ctacgatctg ttcgtattag ttgtcacaaa
    14101 catcttagac agtgattcag aattacttgt tgctggtaaa ggaaaagaaa aagtagaaga
    14161 agctttccaa acaactttga ccaacaaccg tgctttgctt aaaggtgtcg tttcacgtaa
    14221 aaaacaagtc gtacctcaat tgacggctgt atttagctaa tcaaatggta gtatgaagca
    14281 ttcaaaatga atggataagc gattgatata aaaagaaaca tagaacatac taaaggccga
    14341 agaaatggag gagaataaca gtcaattatg actcttgttc ttcctccttt t
//
LOCUS       DACEEZ010000073        14107 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20313_2017
            SAMN12639966-rid9879223.denovo.073, whole genome shotgun sequence.
ACCESSION   DACEEZ010000073 DACEEZ010000000
VERSION     DACEEZ010000073.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639966
            Sequence Read Archive: SRR10023158
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 14107)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14107)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741683.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741683.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:44:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,016
            CDSs (total)                      :: 2,962
            Genes (coding)                    :: 2,889
            CDSs (with protein)               :: 2,889
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 36 of 73
            Pseudo Genes (incomplete)         :: 29 of 73
            Pseudo Genes (internal stop)      :: 18 of 73
            Pseudo Genes (multiple problems)  :: 8 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14107
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639966-rid9879223.denovo.073"
                     /isolate="E20313_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            66..767
                     /locus_tag="IWO89_002172"
     CDS             66..767
                     /locus_tag="IWO89_002172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenine-N(1))-methyltransferase"
                     /protein_id="HAQ0456893.1"
                     /translation="MNHLDLSQRLAAVGDFVPESARLADIGSDHAYLPVALILRGKIE
                     YAVAGEVVQGPYESARKQVKKDGLEDRITVRLANGLDAIQPQDGITAVTIAGMGGSLI
                     RDILEAGKLNQRLSGQERLILQPNIGERTLRSWLQENQYKIQSEKILEENKKIYEIIV
                     AEKEQKPVTYNEMELMFGPFLLEEKSEIFKSKWTRELQQRKAIREQLKQAANQEARQK
                     QIEQEIQMIEEVLKP"
     gene            764..1885
                     /locus_tag="IWO89_002173"
     CDS             764..1885
                     /locus_tag="IWO89_002173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Nif3-like dinuclear metal center hexameric
                     protein"
                     /protein_id="HAQ0456894.1"
                     /translation="MTISGRTFIQKFESYCPQWLAEEGDPVGLHIGTLDKPIQRVMMT
                     LDVRPEVVEEAIKKKIDLLIAKHPPIFRPVKRLVTDQPQEKMYADLLKHDIAVYAAHT
                     NMDIIWDGLNDWFCELLGIEVESYLVKTHEIHYKKLAVYVPVDHAQKMREVLAAAGAG
                     TQGDYTGTSFTSIGHGRFTPEAGAQPAIGKVGKTEQVQEAKVEVILPETIEKQVIQAM
                     RSAHPYEEPAYDLFAIDEPVEMFGLGRVGELPQEISIEAFVEQVKEAFQLDGLRIVQP
                     KNAKSSVKRIAICGGSGEKFYPQAIAQRADVYITGDIYYHTAHDMQSAGLIAIDPGHY
                     IESLCKQRFIEKFESWKQEENWDINFFVSETNTNPFQFN"
     gene            1900..3132
                     /gene="pepT"
                     /locus_tag="IWO89_002174"
     CDS             1900..3132
                     /gene="pepT"
                     /locus_tag="IWO89_002174"
                     /EC_number="3.4.11.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase T"
                     /protein_id="HAQ0456895.1"
                     /translation="MYENLLPRFLRYVKTETRSDATSKTTPSTQTQVAFAQELKKELE
                     ELGLADVFYNEDNGFVMGTLPSNVDHEVRSIGFISHMDTADFNAVGVNPQIIENYDGE
                     STIVLDAEERYTLNTKDFPNLKNYEGHTLITTDGSTLLGADDKSGIAEIMTAMEILIN
                     NPSIPHGTIKVAFGPDEEIGVGADKFDVEQFDVDFAYTVDGGPLGELQYETFSAAQAN
                     ITIQGKNVHPGTAKDTMINALQLAIDFHNQLPADEVPEKTEGYEGFFHLMALDGNPEE
                     ASMSYIIRDHDREKFEARKAQITHIQEELNQRFDQERIKVDMYDQYYNMKEIIEKDMS
                     IVELAKQAMIELGISPVIEPVRGGTDGSKISYMGIPTPNLFAGGENMHGRFEFVSLQV
                     MEKATDVIVKIAELNTKS"
     gene            3264..4172
                     /locus_tag="IWO89_002175"
     CDS             3264..4172
                     /locus_tag="IWO89_002175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YegS/Rv2252/BmrU family lipid kinase"
                     /protein_id="HAQ0456896.1"
                     /translation="MEKLLVFYNKSSGKDEGEKLARWFQDYVKEKQPELYVELAETGP
                     SIDDAELVKKAEAIEADTLVIIGGDGTIHHIVQAFQKTLSQYHVGLLPGGTVNNLARV
                     LEIPLKKEEAADVILEGNVRKIDYGQVNDEVIISTLTIGILADTAVWISQEEKQRYGS
                     WIFIKKFFKLLLKKKKYHLDLETDEEHWKGKSQLLTVTMSNSVGGFTNFDDSATLDDG
                     KFHVTIVPSLDIFRFIFYLPRIMRGKFYTIPGITYFTAKRMKIQAQEKSVGTRTDGDT
                     TDDLPVELSVVSKGLTVYSPKKEEKA"
     gene            4207..4479
                     /locus_tag="IWO89_002176"
     CDS             4207..4479
                     /locus_tag="IWO89_002176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1294 domain-containing protein"
                     /protein_id="HAQ0456897.1"
                     /translation="MDVLLHNLLWFYLFIVNFYLFCLMGYDKHQARTSGWRVPENNLI
                     FVSLLGGGLGGLISRKVFHHKTRKKKFTIGFAAGVLVDLILIYFFH"
     gene            4490..5536
                     /locus_tag="IWO89_002177"
     CDS             4490..5536
                     /locus_tag="IWO89_002177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flippase-like domain-containing protein"
                     /protein_id="HAQ0456898.1"
                     /translation="MSSKGTKTKIILNLLLLIVIFAIIYYLINQSFADIFAELLSTSV
                     VVLAAAVILGTVYQIAEGRSIKEIAEPFAPNFTTKDGFLTSSYIAFYRIVSFGTGTLI
                     SEIYFYNKKGMKLSQGAGVTALHMIMYKLAVITYALAGLVIQFSLFYSNGPSMIWFII
                     AGILLTIVIVAFLLAMSVSLNVQIFFVSLSNKLFKSERLRKWVDTCNQQIYSLRETVQ
                     TIIQDRTALLRIYLWNMLKLVFWYVLPYLILIENHPKIDFLLTISFISFAVVLSGVIP
                     TPAGIGSFEFVYLLLFRPVVGTVDAVSSLLLYRFSSFILPFLYGFVYVIAERRRVIKE
                     EIIEVKKENKENQG"
     gene            5787..8396
                     /gene="clpB"
                     /locus_tag="IWO89_002178"
     CDS             5787..8396
                     /gene="clpB"
                     /locus_tag="IWO89_002178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent chaperone ClpB"
                     /protein_id="HAQ0456899.1"
                     /translation="MNIEKMTTTLQEAIAEAQQIAVTRKHQDIDIAHVWKIFLQPNHF
                     GRNFYTDAGLDVESFEHEIDRLLDEYPVVSGGNVQYGQNLSQNFFRLLNEADQIRESF
                     GDEFLSTEVVILALMKLKNYPLTVYLNKNGLSEKELRKNIEEMRGGERVTSQNQEEQY
                     KALEKYGVDLVQQVKSGKMDPIIGRDEEIRDVIRILSRKTKNNPVLIGEPGVGKTAIV
                     EGLAQRIVRKDVPENLKDKTIFSLDMGALIAGAKFRGEFEERLKAVLKEVKKSDGRIL
                     LFIDEIHNIVGAGKTEGSMDAGNLLKPMLARGELHLIGATTLDEYRQYMEKDKALERR
                     FQKVLVKEPTVEDTISILRGLKERFEIHHGVNIHDNALVAAATLSDRYITDRFLPDKA
                     IDLIDEASATIRVEMNSMPTELDQVTRRLMQLEIEEAALKKESDDASKKRLKNLQEEL
                     AELREEANAMKMQWETEKEEVNSVSAKRAEIDKAKHELEDAENNYDLERAAVLRHGTI
                     PQLEKELKELEAKAKDSEIKMVQESVTENEIAQVVGRLTGIPVTKLVEGEREKLIKLN
                     ETLHKRVIGQDEAVDAVSDAVIRSRAGLQDPNRPLGSFLFLGPTGVGKTELAKALAEN
                     LFDSEDHMVRIDMSEYMEKHAVSRLVGAPPGYVGYEEGGQLTEAVRRNPYTIVLLDEI
                     EKAHPDVFNILLQVLDDGRLTDSKGRVVDFKNTVLIMTSNIGSQLLLEGVTADGTIPE
                     AVAEQVNTLLRGNFKPEFLNRIDDTILFTPLSLDNVKGIVDKMVAQLAQRLEHQEILL
                     TISDEAKTWIAENAYEPAYGARPLKRFITKEVETPLAKEIVAGHVMPKSKVTITLLDG
                     QLHFKTEELEEIV"
     gene            complement(8547..9218)
                     /locus_tag="IWO89_002179"
     CDS             complement(8547..9218)
                     /locus_tag="IWO89_002179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0456900.1"
                     /translation="MIKEIKKVQIALLAFGVFVVCYNFYEFITQKYSTSQGITFIVES
                     LLGIALIFMPQVILKVFKLKIPAAIVLFYWFFLFISVFLGTGMHLISIISFWDKILHA
                     VSPMVLTALGYGLIGYLMKDAEISKTSPWLFLLFGFAFAGLCGVFWEFWEFLCDQFLG
                     MNLQRFAASDGTLFVGRAALMDTMGDLLTNTIGAALMGLFAWSQSKKDERYFESYKLE
                     KVKNT"
     gene            complement(9211..9705)
                     /locus_tag="IWO89_002180"
     CDS             complement(9211..9705)
                     /locus_tag="IWO89_002180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside deoxyribosyltransferase"
                     /protein_id="HAQ0456901.1"
                     /translation="MKIYFAAPMFAKSDLLYNQQLVKEIRELSSELSIYLPQENEAIN
                     DKTAYADSRMIALADTEKVLASDLMIALLDGLTIDAGVASEIGVAYAKGIPVIGLYTD
                     TRQQGGTHPKKIAALQETAENQFHYLNLYTIGLIKLNGKVVSSEIELLSEVKRFLDGG
                     TFSD"
     gene            complement(9725..10945)
                     /locus_tag="IWO89_002181"
     CDS             complement(9725..10945)
                     /locus_tag="IWO89_002181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAQ0456902.1"
                     /translation="MDQKDFRENLELKPVTQEHIDQFNELLSYVFQVTEADIEESGFE
                     NKRAFIRSKKPILEVSKVFGWFHENKLISQIAIYPCEVNIHGARYKMGGVTGVGTYPE
                     YANHGLMQDLIIVALDNMRKNKQWISYLYPYSIPYYRRKGWEIMSDKLSFKIRDTQLP
                     KQIPVSGMVERLPVDHPDVFSVYDKFAQQNHGALFRSDFHWEEYWRFENEDERTAAVY
                     YDSNHEPRGVLFYWVAEEIFHVKEMFYLDQEARNGLWNFISAHFSMIYWVHGDIYKNE
                     PLAFLIEDSQIKEQIEPYFMARIVDVKEFLQRFPFVGTADAFHFIIEDPVAPWNNGVF
                     ALTWDEQGQVRVLNEPIGKSVRLDIQTLTCLMMNYRRASYLARIERLETDEETLKSLE
                     RIIPNMEAYFSDYF"
     gene            11092..12927
                     /gene="lepA"
                     /locus_tag="IWO89_002182"
     CDS             11092..12927
                     /gene="lepA"
                     /locus_tag="IWO89_002182"
                     /EC_number="3.6.5.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor 4"
                     /protein_id="HAQ0456903.1"
                     /translation="MDIKKMKERQEQIRNFSIIAHIDHGKSTLADRILEMTHTVTSRE
                     MQDQLLDSMDLERERGITIKLNAVELNYTAKNGETYIFHLIDTPGHVDFTYEVSRSLA
                     ACEGAVLVVDAAQGIEAQTLANVYLALDNDLEILPVINKIDLPAADPERVRQEIEDVI
                     GIDASEAVLASAKSGIGIEDILEQIVEYVPAPSGDLDAPLKALIFDSIYDSYRGVVLN
                     VRITDGMVKPGDKIKLMSNGKTFDVTEVGVFSPKAVARDFLMVGDVGYITASIKTVQD
                     TRVGDTVTLADNPAAEALPGYRKMNPMVYCGLYPIDTSRYNDLREALEKLQLNDAALQ
                     FEPETSQALGFGFRCGFLGLLHMDVVQERLEREFNLELITTAPSVIYHVNKTDGTTAT
                     VDNPADFPEPVTIQDVEEPFVKAQIMVPNDFVGAVMELSQRKRGEFITMDYLDDYRVN
                     VVYNIPLSEIVFDFFDKLKSSTKGYASLDYEMSGYQKSKLVKMDILLNGEKVDALSFI
                     VHRDFAYERGKAIVEKLKKLIPRQQFEVPIQAAIGQKIVARSDIKALRKNVLAKCYGG
                     DVSRKRKLLEKQKEGKKRMKQIGSVEVPQEAFMAVLKMDEDEPKK"
     gene            complement(13021..14103)
                     /locus_tag="IWO89_002183"
     CDS             complement(13021..14103)
                     /locus_tag="IWO89_002183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017414133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HAQ0456904.1"
                     /translation="MNDDGSRKKVEKVGGDTRPEAEAALRKVLSDIDETGQYFLGTDT
                     RVKQYLDFWMEEYVKLNLKYNTYENYRFTIKNHINGYLGKKKLTDLSPALLQNFINAE
                     FKKGYSKKTMTITHSVLKNALNMAVYPWGLIKQNPMLYVKIPKYEARPTTKKDLKIIS
                     LEDFDHMLEITPEGHPFYIPLNIGFYTGMRVGEVCGLTWDDVDFSNGTITVEKQMVKN
                     DGAWVYGTPKTSSSNRTIFIGQTLLAILKKHKKQQLENRMKYGKLYIDSNAVCTKEDG
                     ELVTPSVVKWNTRRISNALSLSFNFHSLRHTHATLLLENGAKMKEISERLGHSRISIT
                     MDTYSHVTDKMRNETVDIMENLRKNS"
BASE COUNT         4555 a         2592 c         2919 g         4041 t
ORIGIN      
        1 ttttttattc ctagtctcct tattttattt ggaaaatgct ataattatct agaaggagtg
       61 aagctatgaa ccatttagat ttatctcagc gcttagccgc agtgggtgac tttgtccctg
      121 aatccgctcg tttagcagac attggatcag atcacgctta cttacccgtt gcattgatat
      181 taagaggaaa aatcgaatat gcagttgctg gtgaagtcgt tcaaggtcct tatgaatcag
      241 cacgtaagca agtcaaaaaa gatggattag aagaccgtat caccgtacgc cttgcaaatg
      301 gtctagatgc tatccagcca caagatggta ttacagctgt cacgattgcg ggtatgggag
      361 gttcactgat ccgagatatc ctagaagctg gcaagttgaa tcaacgctta agcggacaag
      421 aacgtttgat ccttcagcca aacatcggag agcgtacatt acgatcttgg ctgcaagaaa
      481 atcagtataa aatccaatca gaaaaaatac ttgaagaaaa taaaaaaata tatgaaatca
      541 ttgtagccga aaaagaacaa aaaccagtta cttacaatga aatggagctg atgttcgggc
      601 catttctgct tgaagagaaa agtgagatct tcaaatcaaa atggacaaga gagcttcaac
      661 aaagaaaagc tatccgtgaa cagctcaaac aagcagctaa tcaagaggca cgtcaaaaac
      721 aaatcgagca ggaaatccaa atgattgagg aggttttaaa gccatgacaa tcagcggacg
      781 cactttcatc caaaagtttg aaagctattg tcctcagtgg ctagctgaag aaggagaccc
      841 agtcggactg catatcggaa cattggataa gccaatccaa cgagtaatga tgacattaga
      901 cgttcgtcca gaagtggtcg aagaagcaat caagaaaaaa atcgatttgc tgattgctaa
      961 acatcctcca atctttcgtc ctgtaaaacg gctggttact gatcagccac aagaaaaaat
     1021 gtatgcagat ttgttgaaac atgatattgc cgtctatgca gcacacacga acatggatat
     1081 tatttgggat ggactgaatg actggttttg tgaacttctc ggtattgaag tggagagtta
     1141 tttagtaaag actcatgaga ttcattataa aaaactggcc gtttatgtac ctgtggatca
     1201 tgcacagaaa atgcgtgaag tattagctgc agctggtgct ggtacacaag gtgattatac
     1261 cggtacaagt tttacctcga ttggacacgg tcgatttact ccggaagctg gtgcacaacc
     1321 agcgattggg aaagtaggga agacagaaca agttcaagag gcaaaagttg aggttatttt
     1381 gccggaaact atcgaaaaac aagtgattca agccatgcgg tcagctcatc cttatgagga
     1441 acctgcttat gatcttttcg ctatagatga accagtcgaa atgtttgggt taggacgagt
     1501 aggtgaattg ccgcaagaaa tttctataga ggcatttgta gaacaagtaa aagaagcgtt
     1561 ccaactagat ggactgagaa tagtccagcc aaaaaatgca aaatcctctg tgaagcgcat
     1621 tgcgatttgc ggtggaagcg gtgaaaaatt ttatccgcaa gcaatcgccc aaagagcaga
     1681 tgtctatatc acgggagata tctattatca tacggctcat gatatgcaaa gcgctgggtt
     1741 gattgcaatt gatcccggtc attatattga atcgttatgc aagcaacgat ttatcgagaa
     1801 atttgaatct tggaaacaag aagaaaattg ggatattaac tttttcgttt ctgaaacaaa
     1861 tacaaaccca tttcaattta attaaaagga gagaatacta tgtacgaaaa tcttttgcca
     1921 cgttttttac gatatgtcaa aacagaaaca cgctccgatg caacaagcaa aacgactcca
     1981 tcgactcaga cacaagttgc ttttgcgcaa gaattgaaaa aagaattaga agagttaggc
     2041 ttagctgatg ttttctataa tgaagataac ggattcgtta tgggaacatt accaagcaat
     2101 gttgatcatg aagtgcgttc cattggattt atttcccaca tggatacagc agattttaat
     2161 gcagtaggag tcaatccaca aatcattgaa aactatgatg gcgagtctac gatcgtacta
     2221 gatgcggaag aacgctatac attgaataca aaagattttc caaatttgaa aaattatgaa
     2281 ggccacacat tgatcacaac agatggcagt acattactgg gagcagatga caaatcaggg
     2341 attgcggaaa tcatgactgc aatggagatc ttgatcaata atccttctat ccctcacggt
     2401 accatcaaag tggcttttgg tcctgatgaa gaaatcggtg tcggagcgga taaattcgat
     2461 gttgagcaat tcgatgtaga ttttgcttat acagtcgatg gtggtccttt aggtgagttg
     2521 cagtatgaaa cgtttagtgc tgctcaagca aatattacga tccaagggaa aaatgtacat
     2581 ccagggacgg caaaagatac aatgatcaat gctctacagt tagcaatcga ttttcataat
     2641 cagcttccag ccgatgaagt tccagaaaaa acggaaggat acgaaggttt cttccatttg
     2701 atggcattgg atgggaatcc tgaagaagct tctatgagtt atatcattcg cgatcatgat
     2761 cgtgaaaaat tcgaagcacg caaagcacaa atcacacata tccaagaaga gttgaatcag
     2821 cgatttgatc aagaacgaat caaagtagat atgtatgatc aatattacaa catgaaagaa
     2881 atcattgaaa aagatatgag catcgtggaa ttagcgaaac aagcaatgat cgagcttggt
     2941 atctctccag tgatcgaacc agttcgtgga ggaacagatg gttcaaaaat ctcttacatg
     3001 ggtattccga cgccaaatct atttgccgga ggagaaaata tgcacggacg attcgaattt
     3061 gtttctttac aagtaatgga aaaagcaaca gacgtgattg taaaaattgc agaattgaac
     3121 acaaagagtt aaaatcgaaa aagaacagct tatgacaagg cgttcgtccc aatgatggaa
     3181 tgtcatctgt ctaaggctaa agggcaggac aatggaattg gttgtcccgc cctctttttt
     3241 gaagagtaaa taggaggtaa agaatggaaa agctgctagt tttttataac aaaagttcag
     3301 ggaaagatga gggtgagaaa ttagcacgct ggtttcaaga ttatgtaaaa gaaaagcagc
     3361 cagaattata tgtagaacta gctgaaactg gcccatcgat tgatgatgcg gaattagtca
     3421 agaaagcaga agccatagaa gctgatactt tagtaattat aggtggagac ggcacgattc
     3481 atcatatcgt ccaagcgttc caaaaaacat tatctcaata ccatgtcgga ttacttccag
     3541 gtggaactgt caacaacctt gcacgtgttc ttgagattcc cctcaaaaaa gaagaagctg
     3601 ctgacgtgat cctggaaggc aatgtacgga aaatagacta tggtcaagtc aatgatgaag
     3661 tcattatctc tacactgaca atcggaatct tagcagatac agcagtttgg atcagtcaag
     3721 aagaaaaaca gcgttacggc tcgtggatct ttatcaagaa gttttttaag ttattgctga
     3781 agaagaaaaa atatcatttg gatttagaaa cagacgagga gcattggaaa ggaaaatccc
     3841 aactgttgac agtcacgatg tctaactccg tgggcggttt tacaaatttt gacgactcag
     3901 cgaccctaga tgatggaaaa tttcatgtaa cgatcgtacc ttccttagat attttccgat
     3961 ttatttttta tttgcctaga atcatgagag gcaaattcta tacgatcccc ggcattactt
     4021 atttcacagc caaacgcatg aaaatccaag cgcaggaaaa atctgtgggc actcgaacgg
     4081 atggagacac gacagatgat cttccagttg aattatctgt tgtatcaaaa ggattaacag
     4141 tttattcacc aaagaaagaa gaaaaagctt agaaatagta gttaacagaa agttggaaga
     4201 agagtaatgg atgtattgtt acacaatctt ttgtggtttt accttttcat tgtgaatttt
     4261 tacttatttt gtctgatggg gtatgataaa catcaggcta gaactagcgg ctggcgtgtt
     4321 cctgagaata atttgatatt tgtcagcctg ttaggcggtg gtttgggagg attgatctct
     4381 cgaaaagttt tccatcataa aactcggaaa aagaagttta ctataggatt cgctgcaggt
     4441 gtacttgttg atttgattct catttatttt ttccattaag gagcaggaga tgtcgtctaa
     4501 aggaacgaaa actaaaatca ttctgaatct gcttttgctg attgtgattt ttgccattat
     4561 ttattattta atcaatcaat catttgcaga tatctttgcc gaattgctgt ctaccagtgt
     4621 tgttgttttg gcagcggctg ttatattagg gacagtttac caaatcgctg aagggcgctc
     4681 aatcaaagaa attgctgaac ctttcgcgcc gaattttaca acgaaagatg ggttcttgac
     4741 ctctagctat attgcgtttt accgaatcgt ttcgtttggt acaggaacgc tgatctcgga
     4801 gatttatttt tataacaaaa aaggaatgaa gctatcccag ggagcagggg tgacagcact
     4861 ccatatgatt atgtacaaac tggcagtgat cacttatgca ttagctggct tggtcatcca
     4921 attctctctt ttttattcta atggtccatc aatgatctgg tttatcatcg ctggtatcct
     4981 tttgacgatc gtgattgttg cctttttatt ggcgatgtcc gtcagcttga atgttcaaat
     5041 attttttgtc agcttatcga ataagttatt taaaagtgag cgtttgcgga aatgggtgga
     5101 tacatgcaat cagcagatct actctttaag ggaaacggtc caaaccatta tccaagacag
     5161 gactgcgcta ttgcggattt atctatggaa tatgttaaaa cttgtatttt ggtatgtact
     5221 tccttatttg attctgattg aaaaccatcc taaaatagat tttctactga ccatttcgtt
     5281 tatcagcttt gcagtagtgc tttcaggggt tattcctacg cctgctggta ttggttcctt
     5341 tgaatttgtc tatttgttat tattcagacc agttgttgga acagttgacg cagtttcttc
     5401 gcttttactg tatcgtttca gttcctttat cttgccattt ctctatggat ttgtgtatgt
     5461 gattgctgaa cggcgaagag tgatcaaaga agaaataata gaagtaaaaa aagagaataa
     5521 agaaaatcaa gggtaggtct tttataagat ctaccctttt tatcaaatca aagaaaaacg
     5581 ttatggataa gataatttta cgtttgtttg gctctttttt tatatacata caatcaattt
     5641 tgttttttta agatttatat aagtcgagaa aaaaagtcaa aaaactatgt gtatattttg
     5701 accaaaattg accaaagcga tataatagtt tttgtaaaga tcaaagaaat aagtgatctt
     5761 acctttaatt aggagggtat acctgtatga atattgagaa aatgactacg acgcttcaag
     5821 aagcgattgc tgaagctcaa cagattgcgg tcactcgaaa acatcaagat atcgacattg
     5881 cccatgtatg gaaaatcttt ttacaaccta atcattttgg tcgaaatttc tatacggatg
     5941 ccggattgga tgtcgaatct tttgaacatg aaattgatcg tctactggat gagtatccag
     6001 ttgtttctgg tggaaacgtc caatatggac aaaacctcag ccagaatttc tttcgattat
     6061 tgaatgaagc agaccaaata cgtgaaagct tcggcgacga gtttctttca acggaagtcg
     6121 ttattttagc gttgatgaaa ttaaaaaatt atccgttaac tgtttatttg aataaaaacg
     6181 gattatctga aaaagaattg cgtaaaaaca tcgaagagat gagaggagga gaaagagtga
     6241 cctcacaaaa ccaagaagaa caatacaaag cattagaaaa atacggcgtt gatttagtac
     6301 agcaagtcaa aagcggaaaa atggatccaa tcatcggtcg tgatgaagaa atccgtgatg
     6361 tcatccgtat tctttcacga aaaacaaaaa ataatcctgt tctgatagga gaacccggtg
     6421 tagggaaaac ggcaatcgtt gaagggcttg ctcaacgtat cgtacgcaaa gatgtgcctg
     6481 aaaatttaaa agataaaacg attttttcat tagatatggg tgcattgatc gctggagcaa
     6541 aattccgtgg tgaatttgaa gaaagactaa aagcagtctt gaaagaagtc aaaaaaagtg
     6601 atggacgtat cctcttgttc atcgatgaaa tccacaatat cgtaggggca ggaaaaacag
     6661 aaggaagtat ggatgccgga aatctgttga aaccaatgct tgctcgcggt gaattacatt
     6721 tgatcggtgc cacaacttta gatgagtacc gtcaatatat ggaaaaagac aaagcgttgg
     6781 aacgtcgctt tcaaaaggta ttagtcaaag aaccaacagt ggaagatacg atcagtattc
     6841 tgcgtggatt aaaagaacgt tttgagatcc accatggtgt taacatccat gacaacgcat
     6901 tagtagcagc agctacttta tctgaccgtt atattactga tcggttctta ccggacaaag
     6961 caatcgattt gatcgatgaa gccagtgcaa cgattcgggt agaaatgaac tctatgccaa
     7021 cggaattaga tcaagttact agacgtttga tgcagttgga aatcgaagaa gctgcactga
     7081 aaaaagaatc agatgatgca agtaaaaaac gcttgaaaaa tttgcaagaa gaattagctg
     7141 aacttcgtga agaagcaaat gcgatgaaga tgcagtggga aacagaaaaa gaagaagtca
     7201 attctgtttc agcaaaacgt gctgaaatcg ataaagcaaa acacgaatta gaagatgcag
     7261 aaaataacta tgatttagaa cgcgctgctg tattgcgtca tggtacgata cctcaattag
     7321 aaaaagaatt gaaggaatta gaagctaaag caaaagacag cgagattaag atggttcaag
     7381 agtccgttac cgaaaatgag attgctcaag tcgttggcag attaacgggt atccccgtaa
     7441 cgaaacttgt agaaggtgaa cgagaaaaac taattaaatt gaatgaaaca cttcataaac
     7501 gagtgattgg tcaagatgaa gcagttgatg cggtcagtga cgcggtaatt cgttcacggg
     7561 ctggattgca agacccaaat cgtcctctag gttctttcct tttcctagga ccaactggtg
     7621 tcggaaaaac cgaattagcg aaagcgttag ctgagaactt gtttgactca gaagatcata
     7681 tggtacggat cgatatgagt gaatatatgg aaaaacatgc tgtgtctcgt ctagtcggag
     7741 ctcctccagg ctatgtaggt tacgaagaag gtggtcaatt aacggaagct gtcagacgaa
     7801 atccttatac aatcgtcttg ttggatgaaa tcgaaaaagc gcatcccgat gtcttcaata
     7861 tcttgttaca agtcctagat gacggacgct tgactgattc aaaagggcga gtagtagatt
     7921 tcaaaaatac ggttctgatc atgaccagca atatcggttc acagttactg ctggaaggcg
     7981 tgactgctga tggaacgatt ccagaagcag tggctgaaca agtcaacacg ctattacgtg
     8041 gaaacttcaa accagaattc ttgaaccgaa tcgacgacac gattttattt acaccattga
     8101 gcctagacaa cgtgaaagga atcgttgaca aaatggtggc gcaattggca caacgtctgg
     8161 aacatcaaga aatcctgctg acaatcagtg atgaagccaa aacgtggatt gctgaaaatg
     8221 cttatgaacc agcatatggt gcaagaccat tgaaacgatt tatcactaag gaagtagaaa
     8281 caccactagc taaagaaatc gttgcaggac atgttatgcc taaatcaaaa gtaacgatta
     8341 ctttacttga tggacaactt catttcaaaa cagaagagct agaagaaatc gtctaatctt
     8401 ccattgagac tataaaagaa aaaaggggtc tgacacaact agtgtcagac ctcctttttt
     8461 caaataaaca gtgttccaga agtagtttct tcgaaaaata agccgaaatt tcttgaagct
     8521 gcccgcttct gtcacaacct cttttattag gtatttttca ctttttctaa tttataactt
     8581 tcaaagtaac gctcatcttt tttactttga gaccatgcaa acaatcccat taaggcagca
     8641 cctatcgtat ttgtcagcaa gtcgcccatc gtatccatca atgctgctcg tccgacaaac
     8701 agtgtgccat cagaagcagc aaaacgttga agattcatgc ctaagaactg gtcgcataag
     8761 aattcccaga actcccaaaa cacgccgcaa agtccagcaa acgcaaagcc gaataagagg
     8821 aacaaccaag gactggtttt tgatatctca gcatctttca tcaagtaacc aatcaatcca
     8881 tagccaagag ctgtcaggac cattggactc accgcatgta agattttgtc ccaaaaagag
     8941 atgatactga tcaaatgcat ccctgtgcct aaaaagacag agatgaacaa gaaaaaccag
     9001 taaaacaaaa cgattgctgc tggtatttta agtttgaaaa ctttcaaaat gacttgtggc
     9061 atgaaaatca atgcaatccc taataaagat tccacgataa atgtgattcc ttgagaagtc
     9121 gaatattttt gtgtgataaa ttcataaaaa ttatagcaga caacgaatac accaaaagca
     9181 agtaaagcta tttgcacctt tttgatttct ttaatcactg aaagtccctc catctaaaaa
     9241 tctttttact tctgaaagca actcaatttc tgaagaaacc acttttccgt tcaatttgat
     9301 caaacctatt gtatataagt tcaggtagtg aaattgattt tcagctgttt cttgtagtgc
     9361 agcgattttt ttcgggtggg ttccgccttg ctgtcgagta tcagtatata agccgatcac
     9421 tgggatgcct ttcgcataag ccacaccgat ttccgatgct accccggcat cgatcgtcag
     9481 cccatctagt aaagcaatca ttaaatcact agccaacact ttttctgtgt cagcaagagc
     9541 aatcattcga ctgtctgcat aagcagtttt gtcattgatt gcttcatttt cttgaggaag
     9601 atagatcgaa agctcagaag agagctctct gatttcttta actaactgtt ggttatataa
     9661 caaatcactt ttagcaaaca ttggtgcggc aaaataaatt ttcatctaaa catcatcctt
     9721 tttatcaaaa ataatcgcta aagtaagctt ccatgtttgg aatgatacgc tctagtgact
     9781 tcagtgtttc ttcatctgtt tctaaccgtt cgattcttgc caaataagaa gcacggcgat
     9841 aattcatcat caagcaagtc aatgtttgga tatccagtcg aacggatttt cctataggtt
     9901 catttaagac acggacttgt ccctgttcat cccatgttaa agcgaagaca ccattattcc
     9961 aaggagcgac aggatcttca atgatgaaat gaaaagcatc agctgttcca acaaacggaa
    10021 accgttgaag gaattctttt acatcaacga tccgagccat aaaatatggt tcgatctgct
    10081 ctttgatctg actatcttca atcaaaaatg ctaaaggctc atttttataa atatctccat
    10141 gtacccagta gatcatggaa aaatgtgcgg aaataaagtt ccataagcca tttcgagctt
    10201 cttgatcaag atagaacatt tcttttacgt gaaatatttc ttcagctacc caatagaaca
    10261 gaacacctct tggttcatgg ttgctatcgt agtagacagc ggctgtgcgc tcatcttcat
    10321 tttcgaaacg ccaatactct tcccaatgaa aatcactgcg gaataaagca ccatggtttt
    10381 gttgggcaaa tttgtcataa acggaaaata cgtctggatg atcaactggt aatcgctcaa
    10441 ccataccact tactgggatt tgtttaggta gctgtgtatc tctgatttta aaggaaagtt
    10501 tatcagacat gatttcccat cctttacgcc gatagtaggg gatactgtaa ggatacaaat
    10561 aagagatcca ttgcttattc ttgcgcatat tatccaatgc aacgataatt agatcttgca
    10621 tcaatccatg gttagcatat tctggataag ttccaactcc agtcacacca cccattttat
    10681 aacgtgcacc gtgaatattg acttcacaag gatagatagc gatttgagag atcagtttat
    10741 tttcgtggaa ccatccgaac actttcgata cttccaaaat cggttttttg gaacggataa
    10801 aagctcgttt attctcaaac ccgctttcct cgatgtctgc ttcagtcact tggaacacat
    10861 aggaaagcaa ttcattgaat tgatcgatat gttcttgagt gaccggtttt aattcaagat
    10921 tttctctaaa atctttttga tccatttttt catctccatt tattagcttg ataacagtat
    10981 agcaaaattc tacaaaaaaa acttctatag ctgactaatt aaaaactctt taaattactg
    11041 cttttcgttg gtataatgga aagtgctgat tatatgaaaa gaggagacgc tatggatata
    11101 aagaaaatga aagaacgcca agagcagatt cgtaattttt cgatcatcgc tcatatcgac
    11161 catgggaaat ctacattggc cgatcgtatt ttagaaatga cgcacactgt gacgtctcgc
    11221 gaaatgcaag atcagctgtt agactcgatg gatttggaac gtgaacgtgg aatcacgatt
    11281 aaattaaatg ctgtagaact caattacaca gcgaaaaatg gagaaacata tatctttcac
    11341 ttgattgaca ccccagggca cgtcgacttc acctatgaag tctctcgaag ccttgctgcc
    11401 tgtgagggtg ccgttttagt ggtcgatgca gcacaaggaa tcgaagcaca aacattggcc
    11461 aatgtttatt tagcgttaga caatgatttg gagatcctgc cagtcatcaa taaaatcgac
    11521 ctacccgcag cagatcccga acgtgtccgt caagaaatcg aagacgtaat cggaatcgat
    11581 gctagtgaag ctgtattagc cagtgccaaa tcagggattg ggattgaaga tattctcgag
    11641 cagatcgtgg aatatgttcc tgcaccaagt ggagacttag atgcaccgct aaaagctttg
    11701 atctttgact ctatttatga tagttaccgc ggtgttgttc taaatgtccg gatcacggac
    11761 ggtatggtca aacccggaga caaaatcaaa ctgatgagta atggcaaaac atttgatgtt
    11821 acagaggtcg gggtcttctc gccaaaagca gttgctagag actttttgat ggtcggtgat
    11881 gtcggttata tcacagcaag tatcaaaact gtccaagata cacgagtagg ggatacagta
    11941 acgttagcag acaatcctgc cgctgaagct ttaccaggtt accgaaaaat gaatccgatg
    12001 gtttattgtg gtctttatcc aattgatact tctcgttaca acgatttgcg ggaagcttta
    12061 gaaaaactgc aattgaatga tgctgcttta caattcgaac cagaaacctc tcaagcatta
    12121 ggttttgggt tccgctgtgg attcctcgga cttcttcata tggatgtcgt ccaagaacgc
    12181 ttggaacggg aattcaatct agaattgatc acgactgcac catccgttat ttaccatgtt
    12241 aataaaacgg atggtacgac tgctacagta gataacccgg cagattttcc tgaacctgta
    12301 acgattcagg atgtagaaga accattcgtc aaagcacaga tcatggtccc aaatgatttt
    12361 gtcggtgcag taatggaact ttctcaacga aaacggggag aattcattac gatggattat
    12421 ttagatgatt accgagtaaa tgtcgtctat aatattccgc tttctgaaat tgtctttgac
    12481 tttttcgaca aattgaaatc aagcacgaaa ggctatgcct cattggatta tgaaatgtct
    12541 ggttatcaaa aaagcaagtt agtgaagatg gacatcttgc tgaatggaga aaaagtcgat
    12601 gcattaagct ttatcgttca ccgagacttt gcctatgaac gtggaaaagc aatcgttgaa
    12661 aaactgaaaa aactaatccc acgccaacaa tttgaggtgc caatccaagc agctattgga
    12721 caaaaaatcg ttgcccgttc agacatcaaa gccttgcgta aaaacgtctt ggctaaatgt
    12781 tatggtggag atgtttctcg taaacgtaaa ctcttagaga aacaaaaaga agggaagaag
    12841 cggatgaaac aaatcggctc cgtcgaagtt ccgcaagaag ccttcatggc cgttctgaaa
    12901 atggacgagg acgaaccgaa gaaatagcaa gggtttgttt gaaaacagtg taaaaaataa
    12961 accatgttta catggatttg ccaccggttt gccaccggtg gtttttttcg gtggcaaaaa
    13021 tcaagaattc tttctaagat tttccatgat atcgaccgtt tcatttctca tcttatctgt
    13081 tacatgcgag taagtatcca tcgtaattga aattctgctg tggcccaatc gttcagagat
    13141 ttctttcatt tttgcgccat tttcgagaag aagtgtagca tgagtatgtc tgagagaatg
    13201 gaagttaaaa gagagggaga gtgcattcga tatcctccta gtattccatt tcactacact
    13261 tggcgtaact agctcaccgt cttccttcgt acatactgca tttgaatcaa tgtagagctt
    13321 tccatacttc attcgatttt ctaattgttg tttcttatgt tttttcagaa ttgctagcaa
    13381 ggtttgtcca ataaaaatcg ttcgattgga actgcttgtc tttggcgtac catataccca
    13441 tgcgccgtca ttctttacca tttgtttctc tacagtaatt gttccatttg aaaaatcgac
    13501 atcatcccac gtcagaccac aaacttcgcc aacgcgcatt cccgtataaa atccaatatt
    13561 caaaggaata tagaaaggat gtccttcagg agtaatttct agcatatgat caaagtcctc
    13621 aagagaaatg atttttagat cttttttagt cgttggtcgt gcttcatatt ttggtatttt
    13681 tacatacagc ataggatttt gcttgattaa tccccaagga taaaccgcca tattcagcgc
    13741 attcttaagg acagagtgag taatagtcat tgttttcttc gagtaaccct ttttaaattc
    13801 agcattgatg aaattttgta aaagagcagg ggagagatcc gtaagttttt tctttcctaa
    13861 ataaccgttt atatgatttt tgatggtaaa tcggtagttt tcataggtat tgtattttag
    13921 atttagttta acgtattcct ccatccaaaa atcaaggtat tgttttactc gagtatccgt
    13981 acctaaaaag tattgtcctg tttcgtcaat atctgataaa acttttcgta aagcagcttc
    14041 ggcctctggt cgggtgtctc cgccaacttt ctccactttt tttcttgagc catcatcatt
    14101 gatatct
//
LOCUS       DACEEZ010000074        13817 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20313_2017
            SAMN12639966-rid9879223.denovo.074, whole genome shotgun sequence.
ACCESSION   DACEEZ010000074 DACEEZ010000000
VERSION     DACEEZ010000074.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639966
            Sequence Read Archive: SRR10023158
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13817)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13817)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741683.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741683.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:44:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,016
            CDSs (total)                      :: 2,962
            Genes (coding)                    :: 2,889
            CDSs (with protein)               :: 2,889
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 36 of 73
            Pseudo Genes (incomplete)         :: 29 of 73
            Pseudo Genes (internal stop)      :: 18 of 73
            Pseudo Genes (multiple problems)  :: 8 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13817
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639966-rid9879223.denovo.074"
                     /isolate="E20313_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(143..1345)
                     /locus_tag="IWO89_002184"
     CDS             complement(143..1345)
                     /locus_tag="IWO89_002184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010729379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HAQ0456905.1"
                     /translation="MKTKYPCVYQDKNGRFSYLVELGTDQVTGKRIQKKGTKDSLGKR
                     FSSAREAHKEVMRIKNEYMVVNGFANYDLTYKEFMEQSYVPHYKASVQKSTWSSRECG
                     LIQITEFFGDKKLREINVRDCENYRIWLLNDSGYSQAYSSLMYGMFRKSLDYAVTLQF
                     LNENISKRTKAIPKGKSIVPYWTKEEFEEVLAKIYKDDFYEHMCFLMIWLYYMTGVRV
                     SEGLALNWNDVDFKRKKLRVHHTLEMTNQKDFIRKPYTKTESGLRIISLDDDTIEVLK
                     KWKVIQKNHGVEQFILSYTDLPLYRSTVQRIIQRYAKLAKVPLIQGKGLRHSHVSYLI
                     NEFNADILVVSQRLGHSSPEITLKHYAHLWSRNDEGIAEQMTGNIKFTCATKSKVANF
                     NGNQAVKI"
     gene            complement(1348..1542)
                     /locus_tag="IWO89_002185"
     CDS             complement(1348..1542)
                     /locus_tag="IWO89_002185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3173 domain-containing protein"
                     /protein_id="HAQ0456906.1"
                     /translation="MKNTICRKDLEALGFKTHQATTIIRQAKTRMVNQGYPYYNNKRV
                     GVVPRQVIEEIIGVPLSQEE"
     gene            complement(1589..2437)
                     /locus_tag="IWO89_002186"
     CDS             complement(1589..2437)
                     /locus_tag="IWO89_002186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rep protein"
                     /protein_id="HAQ0456907.1"
                     /translation="MQAYNQKIIETPTYIEIYDYEAIITPKRKTIENEVNLERKQKLA
                     WIYENDITLEKRKNYDELSAQGQYDSLKRKQKHYQSMRFEIARLVDTNFDNQTKFLTL
                     TFKENIQDIEYANNEFKKFIKRLNYDLYKTKKANLKYLATWEKQQRGAIHYHIILFDF
                     PFISFNRLTTIWGQGFVKINKIDVDSIENRGRYISKYFDKELELKEHKKKAFFKSRNL
                     KLPTVTKKMVKKPYDSTNQDILYTSEYIVRRPFFYNALALNELGSPEQVMDFQESTVH
                     YTKIKK"
     gene            complement(2642..2992)
                     /locus_tag="IWO89_002187"
     CDS             complement(2642..2992)
                     /locus_tag="IWO89_002187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002401157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0456908.1"
                     /translation="MPIKITDLSLPKETLEKEYQVVNVTRWQKEGEILGWSYECILPK
                     LRYEKISVKVKSEHPVITPEELTQQGLAIITFTNLVIVPWGRSNGQFVSYGLSATADT
                     ATLIPRKQGTTTQN"
     gene            3587..4033
                     /locus_tag="IWO89_002188"
     CDS             3587..4033
                     /locus_tag="IWO89_002188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cro/Cl family transcriptional regulator"
                     /protein_id="HAQ0456909.1"
                     /translation="MKDYVVQITKEVLEESKTLNKLSSKAKEEAVTILSSGIIDYIEM
                     SGEIKYGEKDRIAKMVKESIKTFDVKQDLTFNDSYLIGKLINILGTDEDTELFLSSLS
                     MIFTDKHFSTRFGGEELVEIFQSMRPALIKLYREKTPDEFYDWFEK"
     gene            4657..5649
                     /locus_tag="IWO89_002189"
     CDS             4657..5649
                     /locus_tag="IWO89_002189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010748893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAQ0456910.1"
                     /translation="MYWEEIELEKTLGPGGEIRAVQRDKKNISKWEKLKKQLRRDKWM
                     YLLLLPGIIMTFIFKYVPMYGAVIAFKDYNPMLGILESPWVGLDHFREFISSPNFTLL
                     LSNTLKISIYGLLLGFFPPIILALSLNQIINKKIKQKIQLILYAPNFISVVIIVGMLF
                     LFLSANGPINGLVEKVTGQPIMFMSNPAYFRTIYILSGIWQGIGWSSILYTATLAGVS
                     TDLYEAANIDGANILQKIWHIDLPALRPVMVISFILAAGGIMNVGYEKAYLMQTSMNI
                     PASEIISTYVYKVGLQSGDYAYSAAVGLFNMIINVVLLLIVNNITKRLNDGQGL"
     gene            5665..6549
                     /locus_tag="IWO89_002190"
     CDS             5665..6549
                     /locus_tag="IWO89_002190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018165023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HAQ0456911.1"
                     /translation="MNSALSKIDRQILWLNRVIVTFLVVITIVPLIYVLVASFMDPAT
                     LINKGISFNPKDWTIQGYQRVFADDSILRGFVNSLFYSFSFSLLTVFITMITAYPLSR
                     PNLAGKKIIMTFFVITMFVGGGLIPTYLLVKNLGMLDSVWAIIVPGAINVWNIILART
                     YFSALPKELSEAATIDGANDLQILFKVMLPLAKPIMFVLFLYAFVGQWNSYFDAMIYI
                     KDPDLQPLQLVLRNILIQNQPSQDMVGANTAMAEMKQIAELIKYATIVISSLPLIIMY
                     PFFQKYFDKGVLVGSVKG"
     gene            6598..8205
                     /locus_tag="IWO89_002191"
     CDS             6598..8205
                     /locus_tag="IWO89_002191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HAQ0456912.1"
                     /translation="MKAKWMLPIVFTSVLILGACGGSKEKSKSSPDYELENVSFPLKE
                     KVSLKMMSQSAPLAPNDPNDKLIFQRLEEETNLHIDWTNYSSDFAEKRNLDIASGDLP
                     DAIFNAAAGDYDLLNWAESGVIVPIEDLIEDYMPNLKKIFDENPEYRQMSTAPDGHIY
                     SMPWIEELGEGKESIHTVNGMAWINKEWLDNLGLEMPQTTDQLMTVLEAFKTQDPNGN
                     GEADEIPFSFINNGGNEDLKFLMGAFGLGDNDDHLVVDNDGQVQFTANKEGYKEAIKY
                     FNEMNNKGLIDKEAFEQDWNTYVAKGKEHKYGVYFTWDKANITGADDSYDVLPVLAGP
                     DGTKHVTRTNGMGFSRDRFVITSANKNLELTARWIDKMYEPLQSVQNNWGTYGDEAQA
                     NIFELKDGMLKHLPLEGTAPGELRQKTEAGGPLAVLDSYYGTVTTMPDDAKWRLDLMH
                     DTYLPYITEDNIYPRVFLEKEETDRLAKIETDMWDYIYRKRAEWITGGKIDEEWEEYL
                     AELERVGLEEWIQIKQTGYDNFLKGAQ"
     gene            8206..9690
                     /locus_tag="IWO89_002192"
     CDS             8206..9690
                     /locus_tag="IWO89_002192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016621147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 32 protein"
                     /protein_id="HAQ0456913.1"
                     /translation="MLVEETAILDKANRFIEMMRGKVNPQYRQNYHFSAPIGWINDPN
                     GFIYYKGHYHLFYQYYPYDSRWGPMHWGHARSSDLVHWEDLPVALAPDQPYDSGGCFS
                     GSAIERDGKLYLFYTGCQEIDGISHQHQCLAVSEDGIHFSKYSGNPVVAEKQLGENGL
                     IQEFRDPKVIVHNDGYYMVVATKSTQSTGRIVMFQSSDLYTWHFYSVLLEGTSEQGIM
                     WECPDLFHLDGRDVLIMSPIQIKKNGLEYHNISSTMACIGEVNWETGQMIVDNFHEMD
                     FGMDFYAPQTTRDPNGGRVMIAWMQMWDRTLPTHELGHLWAGSMSLPRELRVKKNRLV
                     QKPISTVYSHLVYQQGSENIAVTDTSVVFRNALSDSSYLHLVADLEKAEVFTIELLRS
                     KTDCLKLAYDTATQVFELDRQKLGRELTGAEEDPLNVRKVEVPLIDNQLVLEIFRDTS
                     SIEVFINGLEVMTATFYELEKARDILFHSIGESRIQAFETASVK"
     gene            9867..10859
                     /locus_tag="IWO89_002193"
     CDS             9867..10859
                     /locus_tag="IWO89_002193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005230454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family transcriptional regulator"
                     /protein_id="HAQ0456914.1"
                     /translation="MKATMKDVAKLAGVGVGTVSRVLNNGSVKASTREKVEAAMTELN
                     YQPDFYARGLKMKHTNTVALILPTVWHPFFGEFAYHVETALERQNYKLYLCNSSGNSE
                     KEQEYIQMVSQNKVDGIIGITYSDIDEYISSNLPFVSIDRYFTEEVIYVTADNAAGGR
                     LAANELYRRGCQNVAYIGGYQETPNETKSRRKYFEIECRQLGISYKALDMLEPLDHLS
                     ERVTNFLLENPDIDGIFTINDPMALAVIDMLEKIGKNVPEDVQVIGFDGQKMTEGDPY
                     LVSTIAQPTKQMAEVAVAKLIAVINGETVEKRTVLPIEFGEGDTTKPLVRNDFA"
     gene            10911..11780
                     /locus_tag="IWO89_002194"
     CDS             10911..11780
                     /locus_tag="IWO89_002194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010748897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HAQ0456915.1"
                     /translation="MLYGGIEAGGTKFVCGIGNEQLQVIERKSFPTTTPEETMEQVFD
                     FFDKYKGNIASIGVGAFGPIDIQIESRTYGYITNTPKLAWQNYHFVGALENALSVPIY
                     WTTDVNAACYGEYMAGYGKGKNSVLYYTVGTGVGGGAVVDGKIVAGFSHPEMGHMLLQ
                     RHNEDQFEGICPFHHDCLEGLASGPAIEKRFGRKAQTISFDHPYWTIEADYLAQCVYN
                     TTLLYAPEVIILGGGVMKQPQLLELIRQSFKEKMGSYVDTPTLEEYLLTPRLADNAGL
                     IGCLALAIKKLNH"
     gene            complement(12200..13765)
                     /gene="guaA"
                     /locus_tag="IWO89_002195"
     CDS             complement(12200..13765)
                     /gene="guaA"
                     /locus_tag="IWO89_002195"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HAQ0456916.1"
                     /translation="MSSPTRFDEEPKIIVLDYGSQYNQLITRRIREFGVFSELMSHRI
                     TAEEVKAMNPKGIILSGGPNSVYDEKAFGIDEKIFDLGIPVLGICYGMQLITYKLGGK
                     VKPAKNREYGKAELEITSAEAALFQNTPEKQTVWMSHGDLVTQAPTDFDVVATSKDCP
                     IASIQNAERKMYGIQFHAEVRHSEYGNDLLRHFTLDVCQCTGDWTMDNFIDMEIAKIR
                     EQVGDKKVLLGLSGGVDSSVVGVLLQKAIGDQLTCIFVDHVLLRKGEAEQVMESLGGK
                     FGLNIIKVDAKERFLSKLAGVSDPEEKRKIIGNEFVYVFDDEATKLAGEEGVSFLAQG
                     TLYTDVIESGTETAQTIKSHHNVGGLPEDMQFKLIEPLNTLFKDEVRELGTQLGMPDS
                     IVWRQPFPGPGLGIRVLGELTEEKLEIVRESDAILREEIANAGLDRDIWQYFTVLPGI
                     RSVGVMGDGRTYDYTVGIRAVTSIDGMTADFARIPWEVLQKISVRIVNEVAHVNRIVY
                     DITSKPPATVEWE"
BASE COUNT         4234 a         2339 c         2853 g         4391 t
ORIGIN      
        1 ttgaaaaacg ttgatttatc ggcattcccc atccatatta tatttacgtc ttattcccac
       61 tcaatatgag tagcctcgta aagaagctat tatatcagtg ctttttcagc ctattcctca
      121 ttttggtagg gaaatggtag ggttagattt ttactgcttg atttccatta aagtttgcaa
      181 ctttagattt tgttgcacag gtaaatttaa tgtttcctgt catttgttct gcaattcctt
      241 catcattacg gctccataga tgagcgtagt gctttaaagt gatttcaggg ctagaatggc
      301 ctaaccgttg cgacacgacc agaatgtctg cgttaaactc attgattagg tagctaacat
      361 gagaatgacg taatcctttt ccttgtatta ggggtacctt agctagtttg gcataacgct
      421 gaataattcg ttgaacagtt gaacgataga gtgggaggtc agtgtagcta agaataaatt
      481 gttctacacc atgatttttt tgaataactt tccacttttt tagcacttca attgtatcat
      541 cgtccagaga aatgatccgc aagccgcttt cagtttttgt gtaaggtttc cgaataaaat
      601 ctttttgatt tgtcatttcc aaggtgtgat gaaccctcaa ctttttacgt ttgaagtcca
      661 cgtcattcca attaagtgct aatccttcgc ttacacgaac accagtcatg taatatagcc
      721 agatcattaa aaaacacata tgttcgtaga aatcatcttt ataaattttt gccaaaactt
      781 cttcaaattc ttcttttgtc cagtaaggaa cgattgattt tccttttgga attgcttttg
      841 ttcttttgga aatattctca ttcagaaatt gcagggtgac cgcatagtct aatgatttac
      901 gaaacatacc gtacataagt gaactgtatg cttgtgagta tccgctatcg ttcaataacc
      961 aaattcgata attttcacaa tctcgcacat tgatttcacg aagcttctta tcaccgaaaa
     1021 attcagtaat ctgaatcaat ccacactcac gagaactcca agtacttttt tgtacgctag
     1081 ctttgtagtg tggaacgtaa gactgctcca taaattcttt gtaagttaaa tcataattcg
     1141 caaatccatt tacaaccatg tattcatttt ttatacgcat gacttctttg tgtgcttcac
     1201 gagctgatga gaatcgtttt cctagagaat cttttgttcc tttcttctgg atcctctttc
     1261 cagtcacttg atctgttcct aactcaacta gataagaaaa gcgaccattt ttatcttgat
     1321 acacacaagg gtattttgtt ttcatagtta ttcctcctgt gaaagaggaa cacctataat
     1381 ttcttcaatt acttgtcggg gtactacccc aactctttta ttattataat atggatatcc
     1441 ctgatttacc attcttgttt ttgcttgtcg gattattgtg gtagcttgat gtgttttaaa
     1501 tcctagagcc tctaaatctt ttcgacaaat tgtatttttc attgtataaa ctcactttca
     1561 aagaaaacac tatcattggg taatagtgtt atttctttat ttttgtataa tgaacggtac
     1621 tttcttgaaa atccatcact tgttcaggac ttcccaactc atttaaagct aaagcattgt
     1681 aaaaaaatgg acgtctaact atatattcgc ttgtatatag tatatcttga ttcgtggaat
     1741 catagggttt ctttaccatc tttttggtga cagttggtag tttgagattc cgggacttga
     1801 aaaaagcttt tttcttatgc tctttcaatt ccagctcttt atcgaaatat ttgctgatat
     1861 agcgcccgcg attttcgata ctgtctacat cgatcttgtt gatcttaacg aaaccctgac
     1921 cccaaatcgt agttaaccgg ttaaatgaga taaaggggaa atcaaacaag ataatatggt
     1981 agtggattgc accgcgttgc tgcttttccc acgtggcgag atactttaaa tttgcttttt
     2041 tagttttata gaggtcataa ttcagtcgtt tgataaattt tttaaattca ttatttgcat
     2101 actcaatgtc ttgaatgttt tctttgaaag tgagagttaa aaatttcgtt tgattgtcaa
     2161 aattcgtgtc tacaaggcgg gcaatctcaa atcgcatact ttgataatgt ttctgtttgc
     2221 gttttaaact gtcatattgt ccttgagcgg ataattcatc atagtttttt cttttttcaa
     2281 gagtaatatc attttcataa atccaagcta atttttgttt tcgttccaga tttacttcgt
     2341 tttcgatcgt tttacgtttg ggagtaatga ttgcttcata atcataaatt tcaatatacg
     2401 tgggagtttc aatgattttc tgattataag cttgcaaaaa tcatcactcc ttgttgcgta
     2461 ttcgattgtc aaagaaccag tcattttctc ctgatgttga tctataaatc aagttaaggg
     2521 ggaaggcctg cgtagcagcc cttctagcct atggggtgtt gggcggtctt aacggacaga
     2581 aacggctctg tgggcgctgc cccgccgttt cgtcctaacc acccaacact ccatagtata
     2641 gttagttttg tgtagtggta ccttgttttc tgggaatcaa agttgccgta tcagcggttg
     2701 ccgataaccc ataactgaca aattgtccat tcgagcgtcc ccaaggtaca atcaccaaat
     2761 tggtaaatgt aattatggct aaaccttgtt gggtaagttc ttccggcgtg atcacaggat
     2821 gttcagattt gactttgaca ctgatttttt cgtagcgaag ttttggtagg atacattcgt
     2881 agctccaacc tagaatttcg ccctcctttt gccagcgagt gacattgacg acttggtatt
     2941 ctttttcgag tgtttctttt ggtaaactga gatcggtaat ttttattggc atcacaattc
     3001 tcctttttta tacgaaagtt tatacgattt gattaaaaaa tatagatggc catctacgac
     3061 ttgtattata tacgagaact tatacgtagt caagagattt tctaaaaaaa tatatacgat
     3121 tttttatacg agtgttataa tgaaaaaaat gataaggagg aatttgaata tgtttggttt
     3181 agtacaacct aataaagtga aggtgggcca acgaatcaaa gaaataaaag acggaatgtc
     3241 tctctctttt acagaattag ggaatcgatt aggtttaaaa aagccgacga tcagttccta
     3301 cgttcaaggc tatgcgttgg ctcctaagag tgtgatcaac caattatcta gtatcagcgg
     3361 aaaacctgtc ggctggtttt attttgggca tgtggaagaa tatatccgag agtatttgtt
     3421 tctcattggt cagaaaaaaa tagtggacga ttatcctgaa atcattgaga aaataaagca
     3481 agaattttat acgggcgagt tcaaaaatcc tgcttgggag aatgagctag gctatccttt
     3541 ggaggaattt atccaagaat gttttgtaga atattcgagt gaaatcatga aggactatgt
     3601 cgtacagatt acgaaagagg tacttgaaga atccaaaacg ctaaacaaac tatcatcaaa
     3661 agcaaaagaa gaagctgtca cgattctttc atctgggatc attgactata tcgagatgag
     3721 tggtgagatc aagtatggag agaaagatcg cattgcgaaa atggtgaaag aatcaataaa
     3781 aacctttgat gtgaaacaag acctgacatt taatgattcc tatttgattg gaaagttgat
     3841 caatattctt ggtacagatg aagatacgga gctgtttctt tcttctttat caatgatatt
     3901 tactgataag catttttcca ctcgatttgg tggggaagaa ttagtcgaaa tatttcaatc
     3961 gatgcgacca gcgttaatca aactctatcg ggagaaaaca ccagatgaat tttatgattg
     4021 gtttgagaag taaaataaga aaagtggctt agattacaag ttggtgaaaa aatttttttc
     4081 agcataacat cagtaatcta taaaggggtt gttgattata ctgtagaagt taagtactca
     4141 agagattttt tgtacgtaaa caaagcaaaa aagggtgcag tttcactaga aaatgtactc
     4201 ttttttgtga tttttcttag ttatagagtt tttttgaaac tttaaaaatg agaaaacata
     4261 ttgattttaa agagtgtaga catagcttct tttgctaaat agattaaact tactggtgag
     4321 tggtcttgaa catactttta aatcacattt tactgagttt tattggattt tataattcat
     4381 tggaaacctc gtacttaatc tgtttttatc tatcgactag aatctgtata tattccagct
     4441 atcaatgagg ccgcagaaaa gggtgcttaa aaataatctg ttgtttaaaa tattattatc
     4501 agaatactta gatacaaaat tctggcacaa agtaaaaaaa atattgactt aaaaaaatgg
     4561 atatggtaat ctataaacga aaatagtggt aacgtttcca aaatgcatct tcgttaaatt
     4621 tttatttttt taaacgaaaa tggaaacgtt tctaaaatgt attgggagga gattgaattg
     4681 gaaaagacat tgggaccagg tggtgagata agggcggtac aacgggataa aaaaaatatt
     4741 tcgaagtggg aaaaattaaa gaaacagttg cggcgtgata agtggatgta cttgttgctc
     4801 ttaccaggta tcatcatgac gtttatcttt aagtatgtgc caatgtacgg tgctgtgatt
     4861 gcatttaaag actataatcc tatgctagga attttggaaa gtccttgggt aggactagat
     4921 cactttaggg aatttatcag ttcaccaaat tttacgcttt tgctgagcaa cacattaaaa
     4981 attagtattt atggtttatt gttaggtttt tttccaccaa ttattttagc actgagcttg
     5041 aatcaaatta tcaataaaaa aataaaacag aaaatccagt tgattctata tgcgccgaat
     5101 tttatctcag tagttattat tgtggggatg ctgtttttgt tcctatccgc aaatggacct
     5161 attaatgggt tagtagaaaa ggttacgggg caaccaatta tgttcatgtc taatccagct
     5221 tatttcagaa cgatttatat tttatcggga atttggcaag gaatcggctg gtcatccatt
     5281 ctttacaccg caacgctggc gggagtcagt accgatttat atgaggcagc aaatatcgat
     5341 ggggcaaata ttttacaaaa gatttggcat atcgatttgc ctgcattgag gcctgtgatg
     5401 gtgatcagct tcatcttggc cgccggcggt attatgaatg taggatatga aaaagcctac
     5461 ttgatgcaaa cttcaatgaa catccctgcc tcagaaatca tttccactta tgtatacaaa
     5521 gtcggcttgc aatcaggtga ttatgcctac tctgctgctg ttggtctgtt caatatgatt
     5581 attaatgtcg ttttattact gattgtcaac aatataacca agcggctaaa cgatggtcaa
     5641 ggcttgtaat gaaaggagaa aattatgaat agtgctttat caaaaatcga ccgtcaaatc
     5701 ttatggttga atcgagtgat tgtaactttt ttagtcgtca tcaccattgt tccgctgatt
     5761 tatgtactgg tagcttcttt tatggaccca gctactttga tcaataaagg tataagcttc
     5821 aatccgaaag attggacaat acagggatat caacgggtat ttgcagatga ttccatttta
     5881 cgcggttttg taaattcgtt gttttactct tttagcttca gcttattgac tgtatttatt
     5941 acgatgatca ctgcgtatcc tttgtctcga cctaacttgg cggggaaaaa gattattatg
     6001 actttttttg tcattacaat gttcgtgggt ggtggcttga tacctaccta tttattggta
     6061 aagaacttgg gcatgttaga tagtgtatgg gcgattatcg taccaggtgc tataaatgtg
     6121 tggaatatta tcttagcccg tacctatttt agtgctttgc caaaagaact ttctgaagct
     6181 gctactatcg atggcgccaa tgacttgcaa atactgttca aagtcatgct tccattagcg
     6241 aaaccaatta tgttcgtatt gtttttatat gcctttgttg ggcaatggaa ctcatacttt
     6301 gatgccatga tttacatcaa agatcccgac ttacagccgt tgcaactcgt attaagaaat
     6361 attttgatcc aaaaccaacc ttcacaagac atggtgggag ccaatactgc catggcagaa
     6421 atgaaacaaa ttgctgagtt gatcaaatac gcaacgatcg tcatttctag tctgccatta
     6481 atcataatgt atcctttctt ccaaaaatat tttgataaag gtgttttagt tggttctgtt
     6541 aaagggtaag tattgttcac tatttgtatg aataaaaatt ataaaggaga ttttaagatg
     6601 aaagcaaaat ggatgctgcc aatcgttttt acttccgtac taattttggg ggcttgtgga
     6661 ggctcaaaag aaaagagtaa atcaagccct gattatgaac tagaaaatgt ctcttttcca
     6721 ttgaaagaaa aagtatcttt aaaaatgatg tctcaaagtg cccctttggc tcccaatgat
     6781 cctaatgaca aattgatttt ccaacgatta gaagaagaga ccaatctcca tattgattgg
     6841 acaaactaca gtagtgattt tgccgaaaaa agaaatttgg atatcgctag tggtgattta
     6901 ccagatgcca tcttcaacgc cgctgcaggt gactatgatt tattaaactg ggcggaaagt
     6961 ggcgttattg taccgattga agatttgatc gaagattata tgccaaatct gaagaaaatt
     7021 tttgatgaaa accccgaata ccgacaaatg tctactgcac ctgatggtca catttactct
     7081 atgccttgga ttgaagaatt aggcgaagga aaagagtcga ttcatacagt aaatgggatg
     7141 gcttggatca acaaggagtg gttggataat ttgggtcttg aaatgccgca aaccactgat
     7201 cagttgatga ctgtgttgga ggctttcaag acgcaagatc caaatggtaa tggtgaagca
     7261 gatgaaattc cttttagctt tattaacaat ggaggaaacg aagacttaaa atttttaatg
     7321 ggggcattcg gtctaggtga taatgatgac cacttagtcg ttgataacga tggtcaggta
     7381 caatttactg ctaacaaaga agggtataaa gaagccatca aatatttcaa tgaaatgaat
     7441 aataaaggtc ttattgataa agaggctttc gaacaagatt ggaatacgta tgttgccaaa
     7501 gggaaagaac ataagtatgg cgtgtacttt acttgggata aagcaaatat cacgggtgca
     7561 gatgatagtt atgacgtctt gccagtctta gcgggaccag atggaacgaa acatgttact
     7621 cgtacgaatg gcatgggttt ctcaagagat cgtttcgtaa ttactagtgc gaataaaaac
     7681 ttggaactta cagctaggtg gatagataaa atgtatgaac cgttacagtc tgtccaaaat
     7741 aactggggaa cctacggtga tgaagcacag gcgaatattt ttgaattaaa ggatggtatg
     7801 ttaaaacatt tgcctttaga ggggacagca ccgggggaac taagacaaaa aaccgaagct
     7861 ggaggaccat tagcagttct tgattcttat tacggcacag tcactactat gccagatgat
     7921 gcgaagtggc gtttagattt gatgcatgac acgtatttgc catatattac agaagacaat
     7981 atctatccac gtgtcttttt agaaaaagaa gaaacagatc gattagcaaa aatcgaaacg
     8041 gatatgtggg attacatcta tcgcaaaaga gcagagtgga tcaccggcgg taaaatcgat
     8101 gaagaatggg aagaatacct tgctgaattg gaacgtgtag gtttggaaga atggattcaa
     8161 ataaaacaaa ctggctatga taattttttg aagggagcac aataagtgtt agtagaagaa
     8221 acggcgattt tggataaagc aaatcgattt attgaaatga tgcgaggaaa agttaaccct
     8281 cagtatcgac aaaactatca tttttccgca cccatcgggt ggataaatga ccccaatggg
     8341 tttatatatt acaaagggca ttaccatctc ttctatcaat actatcctta cgatagtcga
     8401 tgggggccca tgcattgggg gcatgcgcgc agtagtgatt tggtccattg ggaagatcta
     8461 ccagtagcgt tagccccaga tcaaccttat gacagcggcg ggtgtttctc aggaagtgca
     8521 attgaaagag acggaaaact atatttattt tatacaggct gtcaagaaat tgatggtatt
     8581 agccatcagc atcaatgttt ggcggtctct gaagatggta ttcattttag caaatacagt
     8641 ggtaatcctg tggtagcaga aaaacagcta ggtgaaaatg ggctaataca agaatttcgt
     8701 gatcctaaag taattgttca caacgatggt tactatatgg tagtagctac taaatctaca
     8761 cagtctactg gtagaatagt gatgtttcaa tcgtcagatt tgtatacttg gcacttttac
     8821 tctgtgctgc tagaagggac ttcagagcaa ggaataatgt gggaatgtcc tgatttattc
     8881 catttagacg gacgagatgt cttaatcatg tctccgatac aaatcaagaa gaacggactg
     8941 gaatatcata atatcagttc taccatggct tgtattggtg aggtaaactg ggaaacaggt
     9001 cagatgatag ttgataattt ccatgaaatg gattttggaa tggattttta tgcaccacaa
     9061 acaacaagag atcctaatgg tggacgggtg atgattgctt ggatgcagat gtgggatcgt
     9121 acattgccta cacatgagtt aggccatctt tgggcaggct ctatgagttt gccccgagag
     9181 ttaagagtga aaaaaaatcg attggtacaa aaacccatct caactgtata tagtcattta
     9241 gtttatcaac aaggcagtga aaatattgca gtgactgaca catcagtcgt atttcgaaac
     9301 gcgctgtctg attcttcata tttacatcta gtcgcagatt tggagaaagc tgaagttttt
     9361 acaattgaat tactgcggtc taagacggat tgtctcaaac tagcctatga cactgctaca
     9421 caggtttttg aattggatcg tcagaaacta ggaagagagc ttacaggggc ggaagaagac
     9481 cctctgaatg tacgaaaagt tgaagtacct ttgattgata atcagctcgt tcttgagatt
     9541 tttagagata cctcatcaat tgaagtcttt attaatgggt tagaagttat gacggctact
     9601 ttctatgagc tggaaaaagc tcgagatatc ttgtttcatt cgatcggaga aagtcgtatt
     9661 caagcttttg aaacagcgag tgtgaaataa atgtttgatt gcttaggcga tgaatcaatt
     9721 atttaatagg ttttgaagga taatgttttt agaaaagaac agatagctag atttcccatg
     9781 gatttgctat tagtattttg aaaaaattag taatttatga caaaattcat tatactaaac
     9841 ttgtgcagaa atgtgaggga tcaactatga aagcaacaat gaaagatgtc gcaaaattag
     9901 caggtgttgg agtaggaact gtttcccggg tattgaataa cggttctgtt aaagcttcta
     9961 ctagagaaaa agttgaagca gcaatgacag agttaaacta tcaaccagat ttttatgccc
    10021 gggggttaaa gatgaagcat acgaacacgg ttgctttgat tctacccacg gtatggcatc
    10081 cattttttgg cgaatttgcc taccatgtag aaacagcatt agaaagacaa aactacaagc
    10141 tttatttgtg taactccagt ggtaatagtg aaaaagaaca agaatatatc cagatggtca
    10201 gccaaaataa agtagacggc atcattggaa ttacttattc agatatcgat gaatacattt
    10261 cgagtaatct gccttttgtt agtattgacc gttatttcac tgaagaggtg atttatgtga
    10321 ccgctgacaa tgcggccggt ggacgtttgg ctgcaaatga actttatcgc cgaggatgtc
    10381 agaatgtagc atatatcggt gggtaccaag aaacaccgaa tgaaacgaag agtcgtcgaa
    10441 aatattttga aatcgagtgc cgtcaactgg ggatttccta taaagcgtta gatatgttag
    10501 aacctttaga ccatctctca gaacgtgtaa caaatttttt gttagagaat cccgatatcg
    10561 atgggatttt tacgatcaac gatccaatgg cattagctgt cattgatatg cttgaaaaaa
    10621 tagggaaaaa cgtaccagag gatgtccaag ttatcggatt tgatggtcaa aagatgacag
    10681 aaggagatcc ttatctagtc tcaacgattg cccaacctac aaagcaaatg gcggaagtgg
    10741 cagtggcaaa attaattgct gtcattaatg gagagacagt agaaaaacgt accgtattgc
    10801 cgattgaatt tggcgaaggt gacacaacga aacctttagt gagaaacgat tttgcttagt
    10861 gcaaatacgt ttcttttttt gaaaatgagt gaatgaaagg tgtagataaa atgttatatg
    10921 gtggaataga agccggtgga acaaagtttg tctgtgggat tggtaatgaa caattacaag
    10981 tgatagaacg gaaaagtttt cctaccacaa caccagaaga aacgatggag caagtgtttg
    11041 atttctttga taagtacaaa ggaaatatcg catcaattgg tgtaggagca tttgggccta
    11101 ttgatattca aatcgaatca agaacttacg gatatatcac caacacaccg aagttagctt
    11161 ggcaaaatta tcattttgta ggagctttgg aaaacgcctt gtcggttccc atctattgga
    11221 ctaccgatgt aaatgctgct tgttatggag aatatatggc agggtatgga aaaggtaaaa
    11281 atagtgttct gtattacacc gttggtacag gtgttggtgg tggtgctgta gtggatggga
    11341 aaatagtcgc aggttttagt catcctgaaa tgggacatat gctcttacaa cgtcataatg
    11401 aagatcaatt tgaaggaatc tgtccttttc atcatgattg tttggaaggg ttagcatcag
    11461 gacctgcgat tgaaaaacgc tttggaagaa aagcccagac tatctcattt gatcatcctt
    11521 attggacgat tgaggcagat tatcttgctc agtgtgtgta taacacaacg ttactatatg
    11581 caccggaagt aataatttta ggcgggggtg taatgaaaca gccgcaacta ctcgagttga
    11641 ttcgccaatc gtttaaagaa aaaatgggtt cctacgtgga tacgcctact ctcgaagaat
    11701 atctactaac tccaaggctt gctgacaatg cgggcttgat tggctgccta gctttagcaa
    11761 taaagaaact caatcattag aatagtattt attagaacta ttcataacat accggaagtt
    11821 taaaagggta ttctgaaact taaatagtct caattcattc ttctttcttg agaatctgag
    11881 tagagacttt tattctgttt caaattaata taattacgaa atgatattga gtacaagaaa
    11941 gttcttttta ccagatcttt taaatcgaaa aatctgatat atatatttat agtactaaaa
    12001 atctgattag ataaaaagct aattcaatag gaaaataaag gtagtgaata aaagcaaagc
    12061 ccttgttagt agttaatcta acaagggctt tggtagggaa aatggtagga aaccatctta
    12121 ttttttacga aaatcaggaa ctttagtaat tgaataatgt tgatttatag gtattcctca
    12181 tccatattat gttggctctt tattcccact ccactgttgc aggtggtttg ctggtaatat
    12241 catacacgat acggttaacg tgtgcgactt catttacgat acggacactg attttttgca
    12301 agacttccca agggatacgg gcaaagtcag cggtcatgcc gtcaatagaa gtgacagcac
    12361 ggatacctac agtgtagtca tacgtacggc catctcccat aaccccaaca gagcgaatgc
    12421 ctggtaggac ggtgaagtat tgccagatgt cgcgatctaa accggcattg gcgatttctt
    12481 cgcgcaagat agcgtctgat tcacggacga tttctaattt ttcttctgtc aattcaccaa
    12541 ggacacgaat acctaagcct ggtccaggga atggttggcg ccaaacgatg ctgtcaggca
    12601 tgcctaattg ggtacctaat tcacgtactt cgtctttgaa caatgtgttc aatggctcga
    12661 tcagcttgaa ttgcatgtct tcaggtaatc cacctacatt gtgatgtgat ttgatggttt
    12721 gggcagtttc agttcctgat tcgatcacat cggtataaag ggttccttgt gcaaggaaag
    12781 aaacgccttc ttcaccggca agtttggttg cttcatcatc gaagacatat acaaattcat
    12841 ttccaatgat tttacgtttt tcttctggat cagacacgcc agcaagtttt gataagaaac
    12901 gttctttggc atcgactttt ataatgttca agccaaattt tccgccaaga ctttccataa
    12961 cttgttctgc ttcaccttta cgcaacaata cgtgatcaac gaagatacat gttaattgat
    13021 cgccgatcgc tttttgtaaa agaacaccaa caacagagga atctactccg cctgaaaggc
    13081 ctaataaaac ttttttgtca ccgacttgtt cacgaatttt tgcgatctcc atgtcgatga
    13141 agttatccat tgtccagtca ccagtacatt ggcagacatc tagtgtaaaa tggcgcaaca
    13201 agtcatttcc atattcggaa tggcgaactt ctgcatggaa ttggattccg tacattttgc
    13261 gttctgcatt ttggatcgat gcgatggggc aatctttgct ggtagcaacg acatcgaaat
    13321 cagtcggtgc ttgggtaact aggtcaccat gactcatcca aacggtttgt ttttcaggag
    13381 tgttttggaa aagggccgct tctgcagaag tgatttccaa ttctgctttt ccgtattcac
    13441 ggtttttggc tggtttaact tttccgccta atttataagt aatcaactgc ataccgtaac
    13501 agattcctaa aactgggatc cccaaatcaa aaatcttttc atcaatacca aaggccttct
    13561 catcataaac actgtttggt ccgccagaaa ggatgattcc tttagggttc attgccttta
    13621 cttcttctgc ggtaatgcgg tggctcatca actctgagaa aacaccgaac tcacgaatac
    13681 ggcgagtgat cagttggtta tactgactac cgtaatcaag tacgataatt tttggctctt
    13741 cgtcaaatcg tgttggtgaa gacaaatttg aataaaactt agtccgatat gcggcgcgtt
    13801 ctgtgcgccg catattt
//
LOCUS       DACEEZ010000075        13701 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20313_2017
            SAMN12639966-rid9879223.denovo.075, whole genome shotgun sequence.
ACCESSION   DACEEZ010000075 DACEEZ010000000
VERSION     DACEEZ010000075.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639966
            Sequence Read Archive: SRR10023158
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13701)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13701)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741683.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741683.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:44:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,016
            CDSs (total)                      :: 2,962
            Genes (coding)                    :: 2,889
            CDSs (with protein)               :: 2,889
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 36 of 73
            Pseudo Genes (incomplete)         :: 29 of 73
            Pseudo Genes (internal stop)      :: 18 of 73
            Pseudo Genes (multiple problems)  :: 8 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13701
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639966-rid9879223.denovo.075"
                     /isolate="E20313_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(<1..902)
                     /locus_tag="IWO89_002196"
     CDS             complement(<1..902)
                     /locus_tag="IWO89_002196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside hydrolase"
                     /protein_id="HAQ0456917.1"
                     /translation="MRKVIIDCDPGIDDTLALSLAVKSPDIEVVAITVVCGNVPADIG
                     TQNVLKCLERCDRLDIPVYQGMEKPLKQPFISAQDTHGMDGLGETNFPMILRKQAEPL
                     HAVDFLADYFKEKTNTSVIALGPLTNIAFALKVNPNIGKHMDRFVSMGGTYKSHGNCS
                     PVAEYNYWCDPEAASYVFENLKQTIEMVGLDVTREIVLTPTILEYCCQMNPEEGEYLK
                     AITRFYFDFHWKQERILGCVINDPLAVAYFIEGAICEGFKSFTAVETQGISRGQTLVD
                     RYEFWQKPANSKIMTKVDTRLFSE"
     regulatory      complement(926..970)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00522"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="PreQ1 riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="preQ1"
                     /db_xref="RFAM:RF00522"
     gene            1185..2153
                     /locus_tag="IWO89_002197"
     CDS             1185..2153
                     /locus_tag="IWO89_002197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HAQ0456918.1"
                     /translation="MVIFYFILILIIIGISYHFLSYFYAKKLVRAGVQRGYDWYEDTG
                     HRLMDPAAVTSVEKKRQKLEEECESFWEQGTPKTIKSYDGLKLAGQMFIHPNKQNKWV
                     ICVHDYRSTGKRDMSHIGKRYAEKGFNVLVPDLRAHGESEGEIIGMGWLDRLDLIAWI
                     QLILDEQPNASIILHGGSMGASTIMMASGEKLPSAVKGFILDSGYVSVYAEFRYMLSK
                     ITVFPKKMVMRYANHYAQKYAGYSLKQASATRQLGSNHLPLLVIHGERDHFVPTEAAY
                     TIQNATAGDKALLLVPEAEHLEASMKDPKTYWTVIFSFIEQRITMD"
     gene            2266..2544
                     /locus_tag="IWO89_002198"
     CDS             2266..2544
                     /locus_tag="IWO89_002198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HAQ0456919.1"
                     /translation="MEIKEEKNRLALFNDEQQEIGEMTWSDAGPDIMIIDHTFVDPAY
                     RGQKLAEKLVYTGVELARRDGKKIIPLCPYAKKEFEKKPEYQDVLRQS"
     gene            2662..2898
                     /gene="secG"
                     /locus_tag="IWO89_002199"
     CDS             2662..2898
                     /gene="secG"
                     /locus_tag="IWO89_002199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010718604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HAQ0456920.1"
                     /translation="MYNLILGIVIVLSIVMVIAIMMQPSKQNSAASAFTGGADQLFGK
                     QKARGFEAVMQRSTAVMGAIWMILLFVLAYLSSK"
     gene            3010..3756
                     /locus_tag="IWO89_002200"
     CDS             3010..3756
                     /locus_tag="IWO89_002200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HAQ0456921.1"
                     /translation="MRQLPKPLYAKHGKRAVLLLHAYSGSPNDVRMLARFLEKADYTV
                     YAPLFKGHGTMEPYDILQEKAESWWADTKKAIHFLQSEQFSDIAVLGLSMGGIFAVRA
                     LEEESVIGGGFFCSPLSPVKTNVPENFEKYVRQVLKTAGKSEKEINEKAVAYRSLAEQ
                     QLMDIQDQAAIVESRLSDIQQPIFLAQAGKDEMIDPNGVFETARKLSRQRVTLQWYPE
                     SGHVITVGTARRELEKDVLEFLEKLPWNEE"
     gene            3759..6116
                     /gene="rnr"
                     /locus_tag="IWO89_002201"
     CDS             3759..6116
                     /gene="rnr"
                     /locus_tag="IWO89_002201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease R"
                     /protein_id="HAQ0456922.1"
                     /translation="MTKETIKEKILFFMESSRKRSFSMEEIAEGLGFQKSDDFKLLVQ
                     TIAQMEREQSIVFNKKGKVKLPLKPILIEGTFRANERGFGFVTIDPEEDDIYIPKEAT
                     GFAMDGDTVAIDIMKRANTDMDRGAEGKVVEIRDRATTQLAGEFVAYSEEEIQETDLY
                     GVVIPKDRKLNQFKVYAAAEGVRPVDGSIVLIELTHYPEKGYATSLEGIIKQVIGHKN
                     DPGMDILSIVVANGIPTKFPDDVLSEADEVPDQISEKDITGRRDLRNQLIVTIDGEDA
                     KDLDDAVTVKKLDNGNYFLGVHIADVSYYVTQGSQLDREAYERGTSVYLTDRVIPMIP
                     QRLSNGICSLNPHVPRLTMSCEMEIDPNGQVVSHDIFPSVIQTTERMTYTAVNQILED
                     QDEETMQRYAQLVPMFQEMGELHRILETMRIKRGAISFEDREAKILVDAEGHPNDIQL
                     RSRGIGERLIESFMLAANETVAEHYNKLNLPFIYRIHEQPKEEKMQRFFDFASALGIL
                     VRGTKGTITPKDLQHVIEGVEDKPEAAVINTMLLRSMQQARYSEDNFGHYGLAADYYT
                     HFTSPIRRYPDLIVHRLIRSYSEDKSESNQSSWAAELPEIADHSSKMERRAVETEREV
                     DSMKKAEFMMDKVGEEFDGIISSVVKFGLFIELPNTVEGLIHINELKQDYFHFVENHL
                     ALVGERTGLTFKIGQKVRVKVVKADPDERAVDFELVSAEEVTALERPKARNGKFGKRE
                     GKQAGKRSEKNGKKNKRYSDSKQTFGKKKGKKPFYKDVKKKKKKK"
     gene            6138..6602
                     /gene="smpB"
                     /locus_tag="IWO89_002202"
     CDS             6138..6602
                     /gene="smpB"
                     /locus_tag="IWO89_002202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein SmpB"
                     /protein_id="HAQ0456923.1"
                     /translation="MPKGEGKLIAQNKKARHDYSIIDTMEAGMVLQGTEIKSIRNSRI
                     NLKDGFIRVRNGEAFLHNVHISPYEQGNIFNHDPLRTRKLLLHKKQIVRLENELKNTG
                     ITVVPLKVYIRNGYAKVLIGLAKGKKSYDKREDLKRKDIDRQIDRTLKNFSR"
     gene            6904..7623
                     /gene="atpB"
                     /locus_tag="IWO89_002203"
     CDS             6904..7623
                     /gene="atpB"
                     /locus_tag="IWO89_002203"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HAQ0456924.1"
                     /translation="MDERSLTFHIGPIWFDGTVCIMVLLTCLIVFFLAYFFTRNLKLK
                     PTGKQNAIEWVIDFSRGIVTDNLPRKELSNFHLLAFTLFLFVFVANNIGLVTKIVLPN
                     ETTLWKSPTADPFVTLTLAFIMILLTHLFGVKKLGFKGYFVNSFLKPYSFMFPMKLIE
                     EFTNLLTLALRLYGNIYAGEVLLTLIAKMMTSLGWFSLPLAIPLEMVWIAFSLFIGSI
                     QAFVFVTLSMVYMSHKIEVEE"
     gene            7676..7891
                     /gene="atpE"
                     /locus_tag="IWO89_002204"
     CDS             7676..7891
                     /gene="atpE"
                     /locus_tag="IWO89_002204"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F0 subunit C"
                     /protein_id="HAQ0456925.1"
                     /translation="MNYIAAAIAIMGAAIGAGYGNGQVISKTIESMARQPEMSGQFRT
                     TMFIGVALVEAVPILGVVIALILVFAV"
     gene            7982..8506
                     /gene="atpF"
                     /locus_tag="IWO89_002205"
     CDS             7982..8506
                     /gene="atpF"
                     /locus_tag="IWO89_002205"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit B"
                     /protein_id="HAQ0456926.1"
                     /translation="MLNQLAIAEVGNAMLGNIIVVSGSFLILLALLKHFAWGPISDIL
                     KKREDKIANDLDSAEQSRINSAKMEQEREQQLLASRSDAADIIKNAKESGELSRQNIL
                     KDAQEEAARLKSKAQADITVERDSALNSVKDDVAELSLQIAEKILNKELSPEMHESLI
                     NQYIEGLGSSNETR"
     gene            8493..9035
                     /locus_tag="IWO89_002206"
     CDS             8493..9035
                     /locus_tag="IWO89_002206"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit delta"
                     /protein_id="HAQ0456927.1"
                     /translation="MKLDKYTVGRRYGKALFELAIDSNQAEEIYQDLVSLRQIYEQVP
                     GLGNMLSDVRLEPHEKREIMDKLVSGYDGIVKNFLEVVYEYNRMDDLLFMIEEYERRY
                     DEHQGLLLGSVTTAVPLSDEQRIQLEKNVAKTMDYQSVELKQIVDSSIIGGAIVEANH
                     RVIDGSIRTQLEKMRNQLNR"
     gene            9058..10614
                     /locus_tag="IWO89_002207"
     CDS             9058..10614
                     /locus_tag="IWO89_002207"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HAQ0456928.1"
                     /translation="MAIKAEEISALIKEQIENYQNVLSVEEIGTVTYVGDGIARAHGL
                     ENAMSGELLEFSNGSYGMAQNLESNDVGIIILGDFESIREGDKVKRTGKIMEVPVGEA
                     LIGRVVNPLGQPIDGLGEIVTDKARPVEAMAPGVMQRKSVNEPMQTGLKAIDALVPIG
                     RGQRELVIGDRKTGKTSIAIDTIINQKGQDMICIYVAIGQKDSTVRTQVETLKKYGAM
                     DYTIVVNAGASQPAPLLYIAPYAGTAMGEEFMYNGKHVLIIFDDLSKQAVAYRELSLL
                     LRRPPGREAYPGDVFYLHSRLLERAAKLSDDLGGGSMTALPFVETQAGDISAYIPTNV
                     ISITDGQIFLESDLFYAGTRPAVDAGLSVSRVGGSAQIKAMKKVAGTLRLDLASYREL
                     EAFTQFGSDLDAATQAKLNRGRRTVEILKQKLHAPLAVEKQVVILYALTHGFLDSIPV
                     DSILDFEHELFEYLDTNHSDIFETIRTTKDLPEEEALNSAIQEYKDMFLATKGNNSST
                     EDKLKSIQNA"
     gene            10628..11530
                     /locus_tag="IWO89_002208"
     CDS             10628..11530
                     /locus_tag="IWO89_002208"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit gamma"
                     /protein_id="HAQ0456929.1"
                     /translation="MGASLNEIKTRIASTKKTSQITRAMQMVSASKLTKSEASSQKFQ
                     VYATKVREIVTHLTATQLNDIASENPRGDINYNSMLLSRPVKKTGYIVITADGGLVGG
                     YNSSILKQTMSILEEDHQSPDDYVMIAIGGTGADFFKARGINLAYELRNLSDQPSFDE
                     VRKIVNMATTMYQNEVFDELYVCYNHHINSLTSQFRVEKMLPISDLDPEEATTFEQEY
                     IFEPSKEEILSQLLPQYAESLIYGAIVDAKTAEHAAGMTAMKTATDNAANIIDELTVS
                     YNRARQGAITQEITEIVAGASALE"
     gene            11553..12959
                     /gene="atpD"
                     /locus_tag="IWO89_002209"
     CDS             11553..12959
                     /gene="atpD"
                     /locus_tag="IWO89_002209"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HAQ0456930.1"
                     /translation="MSSGKIVQVIGPVVDVEFSLDQSLPDINNALVVYKNDENKSKVV
                     LEAALELGDGVIRTIAMESTDGLQRGMEVIDTGKAISVPVGKETLGRVFNVLGDTIDL
                     ETPFPEDAERSEIHKKAPAFDELSTSTEILETGIKVIDLLAPYLKGGKVGLFGGAGVG
                     KTVLIQELIHNIAQEHGGISVFTGVGERTREGNDLYYEMKDSGVIEKTAMVFGQMNEP
                     PGARMRVALTGLTIAEYFRDVEGQDVLLFIDNIFRFTQAGSEVSALLGRMPSAVGYQP
                     TLATEMGQLQERITSTKKGSITSIQAIYVPADDYTDPAPATAFAHLDATTNLERKLTE
                     QGIYPAVDPLASSSSALAPEIVGEEHYKVATEVQHVLQRYRELQDIIAILGMDELSDQ
                     EKVLVSRARRVQFFLSQNFNVAEQFTGLPGSYVPVEETVKGFREILEGKYDDLPEEAF
                     RSVGRIEDVVEKAKTLGY"
     gene            12973..13395
                     /locus_tag="IWO89_002210"
     CDS             12973..13395
                     /locus_tag="IWO89_002210"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit epsilon"
                     /protein_id="HAQ0456931.1"
                     /translation="MDQYLTVNVVTPAGLVYDHHAAIVVARTTAGELGILPKHAPIIV
                     PLTIDEVRVKRTDSDTHVDWIAVNGGIMEVRDNVVSIVADSAERERDIDVSRAERAKQ
                     RAERQIEEAKAKQDTNELKRATVALHRAINRIKVSKHS"
BASE COUNT         4530 a         2451 c         2988 g         3732 t
ORIGIN      
        1 ctttcggaaa aaagacgagt atctactttt gtcataattt tactgttagc aggtttctgc
       61 caaaattcat aacgatcaac caatgtttgt ccacgactta ttccctgagt ttccacagcg
      121 gtgaagcttt tgaaaccttc acagatggcc ccctcgatga aataagcaac agctaacgga
      181 tcattgatga cacatcctaa aattctttct tgtttccagt ggaagtcaaa ataaaaacga
      241 gtgatcgctt ttaaatactc tccctcttca ggattcattt gacagcagta ttcgaggata
      301 gtcggtgtca agacgatttc tcttgtcaca tctaacccaa ccatttctat cgtttgtttg
      361 agattctcga atacataaga agctgcttca ggatcacacc agtaattgta ttcagcaact
      421 ggcgaacagt taccatggct tttgtaagtt ccacccatcg agacaaaacg atccatatgt
      481 ttcccaatat ttgggttgac tttcaaagca aaagcgatat tggtcaaagg acccaaagca
      541 atgactgatg tatttgtctt ttctttgaaa taatcagcca aaaaatcaac ggcatggaga
      601 ggttctgcct gttttctcaa gatcatcggg aaatttgttt cccctaaacc atccatacca
      661 tgagtgtctt gtgcactgat gaaaggttgc ttcaaaggtt tttccattcc ttgataaaca
      721 ggaatgtcta aacgatcaca gcgttccagg catttcagca cattctgcgt acctatatct
      781 gcagggacat tcccacaaac tacggtaata gcaactactt cgatatcagg ggatttgaca
      841 gctaatgata aagctaaagt gtcgtcgatc cctggatcgc aatcaataat tacttttcgc
      901 attatttcca atctcctttc tttagagata cttagttttt tattctggga agtttccgaa
      961 ccagtccaag acgagcgtct tgattataat gcacgaaatt agtatactgg cttttattgc
     1021 gaatgtaaat gctctcttac aagtaaatct attgaggaga actttaagta aatgatacaa
     1081 tgaaaaagaa atggaattta cgtgtgccga aaataaagaa tcctatggat cggtacattg
     1141 gataatagat agattatagt ttggcagaaa gcggcgggtg tacaatggtt attttttatt
     1201 tcattcttat tctgataatt atagggatca gttaccattt tctttcttat ttttatgcaa
     1261 agaaattagt aagagcggga gtacagagag gatatgactg gtatgaagac actggtcatc
     1321 gtttgatgga ccctgctgca gtcacgtcag tagaaaaaaa acgacaaaaa ttagaagaag
     1381 agtgtgaaag cttttgggag cagggaacac ccaaaacaat caaaagttat gacgggttga
     1441 aactagctgg tcagatgttc atccatccaa ataagcagaa taaatgggtc atttgcgtac
     1501 atgattaccg aagcacaggg aaaagagaca tgtctcatat aggtaaaaga tatgcagaga
     1561 aaggattcaa cgttcttgta cctgatttac gtgcgcacgg agaaagtgaa ggagaaatca
     1621 tcggaatggg atggctagat cggctagatt taattgcctg gatccaactc atcttagatg
     1681 aacagccgaa tgcgtccatt attttacacg gcggatcaat gggagcttct acgatcatga
     1741 tggcaagtgg tgaaaaactt ccatcagcgg tcaaaggatt tattttagac agcggatatg
     1801 tttctgttta tgcagaattc cgatatatgt taagtaaaat cactgtcttt cctaaaaaaa
     1861 tggtcatgag atatgcgaat cattatgccc agaaatacgc cggttactcc ttaaagcaag
     1921 catcagctac gagacagctt ggcagcaacc atctgccttt attagtcatc catggtgaaa
     1981 gagatcattt tgtccctaca gaagcagcgt acacgattca aaatgctacg gcaggggata
     2041 aagcactgct gcttgttcca gaagcagaac acttggaagc atcgatgaaa gatccgaaaa
     2101 cctattggac agtgattttt tcatttattg aacaaagaat aacgatggat taattttctc
     2161 ttcttttttg taagtaattc gaggataaat aattgtttat agatctggag actgctaaaa
     2221 taaggtagga ataaaaaaat atcagttatt tgaggagtgc ggcatatgga aatcaaagaa
     2281 gaaaaaaatc gtcttgcttt attcaatgat gagcaacaag agattggaga aatgacatgg
     2341 tcagatgcgg gcccagacat catgatcatt gaccatacat ttgtagatcc agcatatcgt
     2401 ggtcaaaagt tagctgaaaa gctagtgtat actggagtag aattagcaag acgagacggg
     2461 aaaaagatca ttcctttatg cccttatgca aaaaaagagt ttgaaaagaa accagaatat
     2521 caagacgtct tacgtcagtc ataagaataa cagctgaaag ttttgaactt tcagctgtta
     2581 ttccatttat atagagttgt cacggacttt tatctatgat aaagttaaag agacgatatc
     2641 ttttgggaag gaggctgcga tatgtataat ttaattttgg gaatcgtgat cgttttatcg
     2701 attgttatgg tcattgcgat tatgatgcag ccaagcaagc aaaatagtgc agcgagtgca
     2761 tttaccggcg gtgcggatca actattcggc aaacaaaaag ctcgcggctt tgaagctgtc
     2821 atgcagcgat caactgcagt tatgggagct atctggatga tcttattatt tgtcctagca
     2881 tatttatctt cgaagtagtt gaataagcct ctcgcaaaac ggcgggcggc tttttttatt
     2941 gcaaagagaa cgcttaagaa gaagttatga aagaaactat ggtaaaatga ggcgaaagag
     3001 gtgagacgag tgagacaatt accaaaaccc ctttatgcaa aacatggaaa gcgagcggtt
     3061 ttgctgcttc acgcttactc aggaagtcca aacgatgttc gaatgctcgc acgatttttg
     3121 gaaaaagcag attatacagt ttatgcgccg ctttttaaag gacacggaac aatggagcca
     3181 tatgatatct tgcaagaaaa agcagaaagc tggtgggcag acactaaaaa agccatccat
     3241 tttttacagt ctgagcagtt ttctgatatt gccgtattag gattatcaat gggcggcatc
     3301 tttgctgtac gagcactaga agaagaatca gtgattggcg gcggtttttt ctgttcgcct
     3361 ttgtctcctg taaaaacaaa cgtgcctgaa aactttgaaa aatacgtacg tcaagtgctg
     3421 aagacagcag gtaaatcaga gaaagaaatc aatgaaaaag cagttgctta ccgctcgtta
     3481 gctgaacaac aattaatgga tatacaagat caggcagcaa tcgtagaaag taggttgtca
     3541 gatatccaac agccgatatt tttagctcaa gctgggaaag atgaaatgat cgaccctaat
     3601 ggcgtatttg aaactgcacg caagttgagc cgtcagcgag tgacacttca atggtatcct
     3661 gaaagtggtc atgtgattac agttggtaca gcaagacgcg aattagaaaa agacgtcttg
     3721 gaatttttag aaaaattacc ttggaatgag gaataaatat gacaaaagaa acgataaaag
     3781 agaaaattct ctttttcatg gaaagcagtc gcaaacgcag tttttctatg gaagaaatcg
     3841 ctgaaggttt agggttccaa aaaagcgatg attttaaatt actagtacaa acgattgctc
     3901 aaatggaaag agaacagagt atagttttca ataaaaaagg aaaagtgaaa cttcctttaa
     3961 aaccaatctt gatcgaagga acatttcgag caaatgaacg aggatttgga tttgtaacga
     4021 ttgatcccga agaagatgac atctatatcc caaaagaagc gactggtttt gcaatggatg
     4081 gagacaccgt agcaatcgat atcatgaagc gagcaaatac agatatggat cgcggagcag
     4141 aggggaaagt agtcgagatt cgagaccggg cgactactca gttagcaggg gaattcgttg
     4201 cttattcaga agaagaaata caagaaacag atctttacgg cgttgttatc ccgaaagata
     4261 gaaagctcaa ccaatttaaa gtatatgcag cagcagaggg agttcgtcca gttgacggaa
     4321 gtattgtgct gatcgaactg acccattacc ctgaaaaagg gtatgcgact agtttagaag
     4381 ggataatcaa gcaagtgatt ggtcataaga atgaccctgg catggatatc ctctcaattg
     4441 tcgtggctaa tggtatccct acgaaatttc ctgatgatgt attgtctgaa gcagatgaag
     4501 ttccagatca aatcagcgaa aaggatataa ctggacgtag agatttaaga aatcagctca
     4561 ttgttacgat tgatggagaa gatgcaaaag atctagatga tgcagttacg gttaaaaaat
     4621 tagataacgg taattatttt cttggagtac acattgcaga tgtttcttac tatgtgacac
     4681 aaggcagtca attggacaga gaagcatatg aaagagggac aagtgtatat ttgactgatc
     4741 gagtgattcc gatgatcccg caacgtttat cgaatggtat ctgttcactg aatccccatg
     4801 ttccaaggct gaccatgagt tgcgaaatgg agattgatcc aaatggacaa gtagtctctc
     4861 atgatatttt cccaagtgtg atccaaacaa cagaacggat gacctacaca gctgtcaatc
     4921 aaatcttaga agatcaagac gaagaaacga tgcaacgcta tgctcagctc gtacctatgt
     4981 tccaagaaat gggtgaattg catcgaatcc ttgaaacgat gcgaatcaaa cgtggcgcga
     5041 tttcttttga ggatcgcgaa gcgaaaatcc ttgttgatgc tgaaggacac ccaaatgaca
     5101 tccagctaag aagcagagga attggtgaac gcttgatcga atctttcatg cttgcggcca
     5161 atgaaacggt agctgaacat tataataaat taaatctgcc gtttatttac cgaatccatg
     5221 aacagccaaa agaagaaaaa atgcagcgct tctttgattt tgcttcagct ttgggcattc
     5281 tggtccgagg aacgaaagga acgatcacac caaaagattt acagcatgtg atcgaaggag
     5341 tagaagacaa accagaagct gcagttatca atactatgtt gctaagaagc atgcaacaag
     5401 cacgctattc agaagacaat tttgggcatt atggcttagc ggcagattac tacacacact
     5461 ttacatctcc tattcgacgc tatcccgatc taatcgtcca tcgtctgatc cgttcttaca
     5521 gtgaagataa aagtgaaagc aatcaaagtt cgtgggctgc ggaacttcct gaaattgcag
     5581 atcatagttc taagatggaa cgtcgtgcag tagaaacaga acgtgaagta gattccatga
     5641 agaaagcaga attcatgatg gacaaagtgg gagaagaatt cgatgggatc atcagctctg
     5701 ttgtgaaatt cggcctgttt atcgaactgc cgaatacagt agaaggattg atccatatca
     5761 atgaattgaa acaggactac ttccattttg ttgaaaatca tttagctctt gtaggtgaaa
     5821 gaactggtct gacttttaaa atcggacaaa aagtgcgcgt aaaagtagtc aaagctgatc
     5881 cagatgaacg agcagtggat tttgaactcg tttccgcaga agaggtcact gcgttggaac
     5941 gtccaaaagc acgcaatgga aaatttggaa aacgagaagg aaaacaagca ggcaaacgct
     6001 cagaaaaaaa tggcaagaag aacaaacgat atagtgatag taagcaaacg ttcggtaaga
     6061 aaaaagggaa aaagccattt tacaaagatg tcaaaaagaa gaaaaagaaa aaataaaatg
     6121 agttggagga aacagctatg ccaaaaggcg agggaaaact aattgcacaa aacaagaaag
     6181 ctcgccatga ttattcgatc atcgacacga tggaagcagg gatggtcttg caaggaacgg
     6241 agatcaagtc gatacgaaac agccggatca atctgaaaga tggatttatt cgcgtccgca
     6301 acggggaagc tttcttgcat aatgttcata tcagtcctta tgaacaagga aatattttta
     6361 atcatgatcc gttgcgcacg agaaagttat tattacacaa gaaacaaatc gttcgtcttg
     6421 aaaacgaatt gaaaaacact ggaatcactg ttgttccttt aaaagtctat attcgtaacg
     6481 gctatgccaa ggtattgatt ggtctggcaa aagggaaaaa atcgtatgat aaacgggaag
     6541 atttgaaacg aaaagatatc gatcgacaaa ttgatcgaac attaaaaaat ttctctagat
     6601 aatttcatta tcctttttcg catattcggt gcttttgctt ttacttaatg ataaaatttg
     6661 ttagcattta atctgataaa acatgattat cacaggtttt ttcttagatc aggagcaccg
     6721 tgcatgggga aaaattcgct tcgtttttga ctgaaataaa aaagtgtcta attttcatga
     6781 aaatgttttg aattttctgt ttcatggtgg tatgataccg tgtggttaga aaccgattgg
     6841 aaaaaaccag atacgcacat gaattgattt tatgtgaatt tcggggaaaa gaggtgaaat
     6901 ggattggatg aacgctcgct aacgtttcac attggaccaa tttggtttga tggtacagtt
     6961 tgtataatgg tgttactgac ctgtttgatt gttttcttct tagcttactt ttttacaaga
     7021 aatcttaagt tgaaaccaac aggcaaacaa aatgcgatag aatgggtcat tgattttagt
     7081 cgaggaatcg taacagataa tctgcctaga aaagaattga gcaatttcca tttgttggca
     7141 tttacattgt ttttatttgt ttttgttgca aacaacatag gtttagtaac aaaaatcgtc
     7201 ttacctaacg aaacaacact ttggaaaagc ccgacagcag acccgtttgt tactttaact
     7261 ttggcattta taatgatctt acttactcac ttattcgggg tgaaaaagtt aggcttcaaa
     7321 ggatatttcg taaattcgtt cttgaaaccc tatagtttta tgtttccgat gaaactgatt
     7381 gaagaattta caaatttatt aacacttgct ttacgtcttt atgggaatat ttatgcgggg
     7441 gaagttttgc taaccttgat tgcaaaaatg atgacaagct taggctggtt ctcattacca
     7501 ttagcaattc cattagagat ggtctggatc gcgttctcct tgtttattgg gagcatccaa
     7561 gcatttgtct ttgtcacttt gtcaatggtt tatatgagcc ataagattga agtagaagaa
     7621 taatactcgg ttaaaaaaca aactactatt tatctatttt aggaggaaaa caattatgaa
     7681 ttatatcgca gcagcaatcg caatcatggg agcagcaatc ggagccggtt atggtaacgg
     7741 tcaagttatc tctaaaacaa tcgaatcaat ggctcgccaa cctgaaatgt ctggtcaatt
     7801 ccgtacaaca atgtttatcg gggtagctct agttgaagct gttcctatct taggggtagt
     7861 tatcgccttg attctagttt ttgccgttta attgaatcag tattaaggca gaaacaacat
     7921 cgtttgttac tgctttttta catggaaact agaaatactc caaagaaagg aaggctcgcc
     7981 tatgctgaat caattggcaa ttgcagaagt tggcaatgcg atgttaggta atatcatcgt
     8041 tgtcagcggc tcatttttga tactactggc tctcttgaag cactttgctt ggggaccaat
     8101 cagcgatatt ttgaaaaaac gtgaagacaa gatcgccaat gatttagatt ctgcagaaca
     8161 atctcgcatc aactcagcga aaatggaaca agaacgcgaa caacaattgt tagcctctcg
     8221 ttctgatgca gctgatatca tcaaaaatgc gaaagaaagt ggagaattaa gccgccaaaa
     8281 tattttgaag gatgctcaag aagaagcagc tcgtctaaaa agcaaagccc aagctgatat
     8341 cactgtagaa cgtgattcag cgctgaactc tgtaaaagac gacgttgcag aactctctct
     8401 tcaaatcgcg gaaaaaatct tgaacaaaga attatctcca gaaatgcatg aatcattaat
     8461 caatcaatac attgaaggtc taggttcttc aaatgaaact agataaatat acagtaggaa
     8521 gacgttacgg gaaagcttta tttgaactgg cgatcgattc aaatcaagca gaagaaatct
     8581 atcaagatct cgtaagttta cgtcaaatct atgaacaagt acctggatta gggaatatgt
     8641 taagtgatgt tcgtctagaa cctcatgaaa aacgagaaat catggacaag cttgtcagcg
     8701 gttatgatgg gatcgttaaa aactttttag aagttgttta tgagtataac cgaatggatg
     8761 accttttgtt catgatcgaa gaatatgaac gtcgatatga tgaacatcaa gggttactat
     8821 tgggaagtgt cacaacagca gtcccacttt cagacgaaca acgaatacaa ttagaaaaga
     8881 atgttgcaaa aacaatggac tatcaatcag ttgaattaaa acaaatcgtt gattcttcta
     8941 ttattggtgg cgcgattgtt gaagccaacc atcgagtaat cgacggaagc attcgcacgc
     9001 aattagaaaa aatgcgtaat caattaaaca gatagtacaa agaggtaggt gaatcgaatg
     9061 gccatcaaag cagaagaaat tagtgccttg attaaagaac aaattgaaaa ttatcaaaac
     9121 gtgctttcag tcgaagaaat cggtaccgta acttatgtag gtgacgggat cgctcgtgca
     9181 catggtttag aaaacgcgat gagtggtgag ttgcttgaat tttcaaacgg ctcatacgga
     9241 atggcgcaaa acttagagtc aaatgatgta ggtatcatta tccttggcga tttcgagtcc
     9301 attcgtgaag gagataaagt aaaacgaaca ggtaagatca tggaagttcc agttggagag
     9361 gccttgatcg gtcgggtagt caatccgcta ggtcaaccaa tcgacggact aggtgaaatc
     9421 gttactgaca aagctcgtcc agtagaagcg atggcaccag gtgttatgca acgtaaatct
     9481 gttaacgaac caatgcaaac agggctaaaa gcgatcgacg cccttgtgcc aatcggacgc
     9541 ggacaacgtg aattagtcat cggtgaccgt aaaacaggga aaacttctat tgcgatcgat
     9601 acgatcatca accaaaaagg tcaagatatg atctgtatct atgtagcaat cggacaaaaa
     9661 gattctacag ttcgtacaca agttgaaaca ttgaaaaaat atggcgcaat ggattacaca
     9721 atcgttgtga atgccggtgc gtctcaacca gcaccattgc tttatatcgc accatatgct
     9781 ggtactgcaa tgggtgaaga attcatgtac aacggtaaac atgtattgat catttttgat
     9841 gatctttcaa aacaagctgt tgcttatcgt gaactttcct tgctgcttcg ccgtccacca
     9901 ggtcgtgaag cttatccagg ggatgttttc tacttacact cacgcttatt ggaacgtgca
     9961 gcaaaattaa gtgatgattt aggtggcggt tcaatgactg ccttaccatt tgttgaaaca
    10021 caagctggag atatctctgc ttatatccca acaaacgtta tttcgatcac tgatggacaa
    10081 atcttcttag aaagcgactt gttctacgca ggtactcgtc cagcggttga tgccggacta
    10141 tctgtatctc gtgtaggggg ttctgcgcaa atcaaagcaa tgaaaaaagt tgctgggact
    10201 ttacgtctag acttagcaag ttaccgtgaa ttagaagcat ttacacaatt cggttctgac
    10261 ttggatgcag caacacaagc aaaactaaat cgtggacgcc gtacagtaga aatcttgaaa
    10321 caaaaattgc atgcaccgct agctgttgaa aaacaagtag tgattttgta tgctttgaca
    10381 catggattct tagatagtat ccctgtagac agtattttag acttcgaaca tgaattattc
    10441 gaatatctgg ataccaatca ttcagatatc tttgaaacaa tccgtacaac aaaagatctt
    10501 ccagaagaag aagcgttgaa cagtgcgatt caggaatata aagacatgtt cttagctaca
    10561 aaaggaaata attcctctac agaagataaa ttgaaatcca ttcaaaatgc atagtgaggt
    10621 gagatgaatg ggtgcttcat taaatgaaat caagacacgt atcgcttcaa cgaaaaaaac
    10681 gagccaaatc acgcgggcaa tgcagatggt gtctgcttct aagttgacaa aatctgaagc
    10741 ttcttcacag aagtttcagg tttatgcaac aaaagtaaga gaaatcgtta cgcatttgac
    10801 tgctactcag ttaaatgata ttgcctcaga aaatcctaga ggggatatca attacaacag
    10861 catgttgctc agccgtcctg ttaagaaaac gggttatatc gtcatcactg cagatggcgg
    10921 tttagtcggt ggatataaca gctcgatatt gaaacaaacg atgtctattt tagaagaaga
    10981 ccaccaatca cccgatgatt acgtgatgat cgcaattggc ggaactggtg cagacttctt
    11041 taaagcacgc ggtatcaatc tagcatatga gctgcgcaat ctatctgatc agccaagctt
    11101 tgatgaagtc cgcaaaatcg taaatatggc aacaactatg taccaaaacg aagtattcga
    11161 tgaattatac gtatgttata accatcatat caattcgcta actagtcagt ttcgtgtaga
    11221 aaaaatgctg ccaatctcag acttagatcc agaagaagca actactttcg aacaagaata
    11281 tatttttgaa ccttcaaaag aagaaatctt gtctcagtta ttgcctcagt atgctgaaag
    11341 tttgatctac ggtgcgatcg ttgacgccaa aacagcagaa catgctgctg gtatgacagc
    11401 aatgaaaaca gcaactgata atgctgctaa tatcattgat gagctgacgg tttcttacaa
    11461 ccgtgcaaga caaggggcta ttacccaaga aattaccgaa attgttgccg gagcttcggc
    11521 actagaatag tattgagatt ggaggaaaaa agatgagttc aggcaagatt gttcaagtaa
    11581 tcggtcccgt tgtcgacgtg gaattttctt tagaccaatc cttaccagat atcaacaacg
    11641 cattagttgt ttataaaaat gacgaaaata aatcaaaagt tgttcttgaa gcagcgttag
    11701 aattagggga cggagtgatc cggaccattg cgatggaatc aacagatggt ttacaaagag
    11761 gaatggaagt cattgataca ggcaaagcaa tctctgttcc tgtaggtaaa gaaacattag
    11821 gtcgcgtatt caacgtacta ggagatacga tcgatttaga aacacctttc ccagaagatg
    11881 cggaaagaag cgaaattcat aaaaaagcac cagcctttga cgaattaagt acaagtacag
    11941 aaattttgga aacagggatc aaagttatcg atttgcttgc cccatattta aaaggtggga
    12001 aagtcggact attcggtggt gccggtgttg gtaaaaccgt actgatccaa gaactgatcc
    12061 ataatatcgc ccaagaacat ggtggtattt ctgtatttac cggtgtaggt gaacgtactc
    12121 gtgaaggtaa tgacttgtat tatgaaatga aagattcagg agttatcgaa aaaacagcca
    12181 tggtgttcgg acaaatgaac gaaccaccag gtgcacgtat gcgtgttgct ttgactggtt
    12241 tgacgattgc ggaatatttc cgtgatgtag aaggtcaaga tgtactgttg tttatcgaca
    12301 acattttccg tttcactcaa gctggatctg aagtatcagc cttgttagga cggatgcctt
    12361 ctgcggttgg ttatcaacca acattggcaa cagaaatggg tcaattgcaa gaacgtatca
    12421 catctacgaa aaaaggttcg atcacatcga tccaagcaat ctatgtgcca gccgatgact
    12481 acactgaccc ggcgccggca acagcattcg cccatttgga tgcgacaacc aacttggaac
    12541 gtaaattaac agaacaaggg atttatccag cggtagatcc attggcttct tcttcaagtg
    12601 ctttagcacc tgaaatcgtg ggagaagaac attataaagt agcaacagaa gtccaacacg
    12661 tattgcaacg atatcgtgaa ttacaagata tcattgctat cttaggtatg gatgaattat
    12721 ccgatcaaga aaaagtgtta gtttctcgtg cccgcagagt ccaattcttc ttgtcacaaa
    12781 actttaacgt ggctgaacaa tttactggtc ttccaggttc atatgtccct gtagaagaaa
    12841 cagtgaaagg attccgcgaa atcttggaag gcaaatacga tgatcttcct gaagaagctt
    12901 tccgaagtgt tggccgaatc gaagacgtcg tagaaaaagc gaaaacacta ggctactaga
    12961 aaggggtgcc ccatggatca atatttaaca gtaaatgtgg taactcctgc cggtttggtc
    13021 tatgatcacc atgcagctat cgtcgttgca cgtacgacag ctggtgagct tgggattttg
    13081 cctaaacatg caccaatcat cgttccattg acgattgatg aagttcgtgt aaaacgaaca
    13141 gattcagata cccacgtcga ttggatcgct gttaatggcg gaatcatgga agttcgtgat
    13201 aatgttgttt caatcgttgc tgatagtgcg gaacgtgaac gtgatatcga tgtatctcgt
    13261 gcggaacgtg caaaacaacg cgcagaacgc caaatcgaag aagcaaaagc aaaacaagat
    13321 acgaacgaat tgaaacgggc aactgttgcg ttgcaccgag caatcaatcg tatcaaagta
    13381 tctaagcact cataaaatga gatgcggatg tttatccgtg tttaagaaca gtaaaaagag
    13441 gttatgacag aagtggtcag cttcaagaca taagaaggaa tttacgaaaa ttgcttctca
    13501 aatttttgtg aaatctcagc ttatttttcg aagaagctac ttctggaaca ccgtatatcg
    13561 gaggttgtga aatcagcgtt ttgtcctgag tattagagaa acaaatggaa aatagttcct
    13621 catattttgc cgttttttga gctaataccg agggtcagat gattgaacac cgtttatttg
    13681 gaataaggga ctgcgacaag a
//
LOCUS       DACEEZ010000076        13682 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20313_2017
            SAMN12639966-rid9879223.denovo.076, whole genome shotgun sequence.
ACCESSION   DACEEZ010000076 DACEEZ010000000
VERSION     DACEEZ010000076.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639966
            Sequence Read Archive: SRR10023158
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13682)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13682)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741683.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741683.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:44:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,016
            CDSs (total)                      :: 2,962
            Genes (coding)                    :: 2,889
            CDSs (with protein)               :: 2,889
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 36 of 73
            Pseudo Genes (incomplete)         :: 29 of 73
            Pseudo Genes (internal stop)      :: 18 of 73
            Pseudo Genes (multiple problems)  :: 8 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13682
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639966-rid9879223.denovo.076"
                     /isolate="E20313_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            39..1403
                     /locus_tag="IWO89_002211"
     CDS             39..1403
                     /locus_tag="IWO89_002211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HAQ0456932.1"
                     /translation="MSQLLIATPFHVLASENSTYKIAKERTETTEMSNSDVTAESSEL
                     LTTSSSESSTTSNAESSTTSSSESSTTSNAESSTTSSSESSTISSTESSTTSSSTSEI
                     TSSKVTKPNHIESSKKEAPKTEGQTKTFSTPQQIESTESFQSQPILSGNEEIHFDKNE
                     SVENFIMKIGESARKIGQKYDLYASVMIAQAILESASGQSQLAQAPNYNLFGIKGTYN
                     GNFVIMVTNEDLGNGTLYTTQSKFRVYENYEESFEDYAKLLTKGISGNKDFYAGALKA
                     NSKTYREATKFLTGRYATDTQYYLKLNELIKTYDLTNYDKEVKGPQMSKTGYQVPIHN
                     YSITSLFGNRGNEFHRGLDMAATQGEPIHAVKAGKVVKAEFHYSWGNYVVIQHEDGST
                     ALYAHQQQYLVKTGDNVLQGQIIGYVGSTGNSTGPHLHLEICKDSSLSQSMLVDPKTL
                     LFGD"
     gene            1752..2384
                     /locus_tag="IWO89_002212"
     CDS             1752..2384
                     /locus_tag="IWO89_002212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AP2 domain-containing protein"
                     /protein_id="HAQ0456933.1"
                     /translation="MKERYGHLTVVEEITHNGYKKYRCLCDCGNETIVYRSNLLSGRT
                     KSCGCGNYKNRHKYKNLTNKTFGKLVAIKPTKLRSEGTVVWECQCLCGRKKLVSAKRL
                     LKGDVKSCGCLKSKKHHIENKKFGLLTAKYPLDNNCSWKTKWVCHCECGSMCEISYSN
                     LFFGHTQSCGCLKRQETRTCVEGTVIECLTSKLPKNNTSGVKGVYRSKEK"
     gene            2972..4411
                     /locus_tag="IWO89_002213"
     CDS             2972..4411
                     /locus_tag="IWO89_002213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016172772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAQ0456934.1"
                     /translation="MYSYQEDAKVQKNRWWILIAISMFTFMSTLDGSIVNIALPTISD
                     DMGIPMNQAEWVVSVYLMIVCSCLLLFGKIGDSRGKIKVFRIGTLIFVLGSLLCGFNH
                     SLGILLFARVVQAIGASMTMATNAGIITEVFPIHERGRALGAIGAFVSLGSIAGPGLG
                     GLILSQFSWAYIFWINVPVGIVTMLIAEKFLPKDITMSGHTIDGGGFAAIAATIISFF
                     GAIFLGQESGFFHPVPLVLFVISVIAFVSFLKVEKKAASPLISFGIFKNKIFTMSLIT
                     ALLIFSSNFFVNVVIPFYLQNARGLSPSYAGLLMMVFPLMMVVGAPLSGYLTDRVGPE
                     ILVLVGLGLLSVTQVMYMFLSVDSPLWLYIVATAIMGAGNALFQSPNNTMVMSSVTIE
                     NLGLAGSMNSFARNLGMVSGISLSTTVLYQAMSAKMGQQVTNYIANRPDVFLYGMKIT
                     FLGSFVLCFVAFFLTIWRIKKQGGIQKNV"
     gene            4706..4924
                     /locus_tag="IWO89_002214"
                     /pseudo
     CDS             4706..4924
                     /locus_tag="IWO89_002214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174271.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            5173..5388
                     /locus_tag="IWO89_002215"
     CDS             5173..5388
                     /locus_tag="IWO89_002215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0456935.1"
                     /translation="MNAGIADMNLIKKTLNDFTSNSISKGTGINLSTIKKLKSGERSV
                     EKLNLLDAIKITEFAMKNGKAEIEIWR"
     gene            complement(5605..5861)
                     /locus_tag="IWO89_002216"
                     /pseudo
     CDS             complement(5605..5861)
                     /locus_tag="IWO89_002216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287522.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
     gene            complement(6048..6959)
                     /locus_tag="IWO89_002217"
     CDS             complement(6048..6959)
                     /locus_tag="IWO89_002217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HAQ0456936.1"
                     /translation="MKIKIERNQKEKSPKKKKERVVSVGKHRKMVLALWLLLSCSLAF
                     GVYKNFTAIDQHTTHEKVVIKEKVVNTSGVESFTEDFAKEYFSWKNNKEAIEKRMSNL
                     EQYLTENGLALIQDMVRADIPTSSEVQSVKILDVEKHSEEFVVSFLVEQHITEGKKTQ
                     SISSAYRVTIFEDENRNHLVTSLPTMIGKPDKAKYEAKQVENDSEIDAKTTEEITDFL
                     ETFFKLYPSASEKELEYYVENGAMRPINIDLKFSELENPVYLQNGKNFLVSTKIKYLD
                     NVSKTTSYFQYKLVLQKNGNWKIVDVK"
     gene            complement(6978..7982)
                     /locus_tag="IWO89_002218"
     CDS             complement(6978..7982)
                     /locus_tag="IWO89_002218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase P60"
                     /protein_id="HAQ0456937.1"
                     /translation="MNLKKVGVISLTFLTISFMGILMCVGLLFGEDSEGNGSSGTSPG
                     GTSVSEEVLKHRAMVEKYAKESDITEYIPILLAIIQVESGGTMEDVMQSSESAGLPPN
                     SLSTEASIKQGCLYFASLVKRSKELGCDQDSIIQAYNYGGGYLDHVGKNGKKHSFALA
                     ESFSKEKSGGQKVDYPNPIAIKENGGWRYNYGNMFYCLLVKQYLTTTQFDDKIVQEIF
                     DEAFKYEGTAYVFGGSSPETGFDCSGLTQWCYAKVDLKLPRVAQDQYDAMTHIDLKDA
                     KPGDLVFFHSTYDAGTYVTHVGIYAGENRMFHAGDPVGWTNLTENYWRQHLIGAGRYK
                     "
     gene            complement(7979..10102)
                     /locus_tag="IWO89_002219"
     CDS             complement(7979..10102)
                     /locus_tag="IWO89_002219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002407825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HAQ0456938.1"
                     /translation="MKKKLFRIVGIVFISILTLIVLLSLVGTVAEATGLVDDTVEAGN
                     LYSKYSLNNYQLDFFVDSSWDWLPWNWGDGLGKSVMYGLYAITNFIWTVSLYLSNATG
                     YVVQEAYKLDFISDTAESIGKNIQTLAGITENGLQTSGFYFGFLLLMILALGVYVAYT
                     GLLKRETTKAVRAVINFVMIFLLSGSFIAYAPTYITKINDFSSDVSEAALTLGTEIVV
                     PNSESQGKDSVDLIRDSLFSIQVQQPWLLLQFDDSNIEEIGEDRVNKILSVSPDENKG
                     KDREEAVKAEIEDNDNANLSITKTMNRLGTVVFLVLFNIGISFFVFLLTGIMLFSQIL
                     FIIFAMFLPISFLLSMLPTYESLGKKAIIRLFNTIMMRAGVTLVITTAFSISTMFFNI
                     SATYPFFMVAFLQIVTFAGIYFKLGDIMSMFNLQSNDSQSMGRRVMRKPQMLMNRKLR
                     QLNRNVGRALAFGGGAAVGNKLAKEQSKSKFKPSGSSLRKNSRLPNDHEILSDLTKEN
                     PVSNNKKQSRMNLAGRKIGKVLDTQALVKDKTKQVKDQVRNTPTNLKYNLHKGIEKSK
                     KAPEEFKRGLFQEKADRVDLREKQRQRRNAKMDEKRKILSEATNRQGKRRANIPIDKA
                     AQPQKDMMLKKEAPKRIVRANLNPEIKRKLTSQEIISKGKSQSASKKNSFQQVKQRST
                     LPKQTNQKVQKRMNHRPKSRSGEKK"
     gene            complement(10107..12554)
                     /locus_tag="IWO89_002220"
     CDS             complement(10107..12554)
                     /locus_tag="IWO89_002220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HAQ0456939.1"
                     /translation="MYPIKYIENNLVFNQEGECFAYYELIPYNYSFLSPEQKYQVHDN
                     FRQLIAQNREGKIHALQIATESSIRATQERSKKEITGRLTEVAKQRIDLQTEALVSMI
                     GDSQIDYRFFIGFKLIATDEEVNLKNLKKSFFSGFQEFVYGVNHHLMGDFVSLSNEEI
                     RRYTKLEKLMESKLARRFKVRRVTPSDLMYLIEHIYGEKGTPFEEYEFQLPKKKLKSE
                     TLVKRYDLLRPSRCLIEEKPRYLCMEHENHESYVAYLTINTIVGEMEFPSSELFYYQQ
                     QQFTFPIDTSMNVEIVTNKKALATVRNKKKELKDLDNHAYQSDNETNSNVLDALDSVD
                     ELETTLDQSKESMYKLSYVVRVSAESVDELKRRCDEVLDFYDDTNVKLVRPFGDMMGL
                     HEEFLPSSKRYMNDYIQYVTSDFLAGLGFGATQMLGELEGIYFGYNVDTGRNVYLKPA
                     LASQGVKDSVTNALAAAFLGSLGGGKSFSNNLLVYYAVLFGGQAVIVDPKGERGGWKE
                     TLPEIADEINILNLTSKPKNQGLLDPYVIMEKKKDSESLAIDILTFLTGISSRDGEKF
                     PVLRKAIRTVTQSNQRGLLQVINELRKEGSPVAENIADHIESFTDYDFAHLLFSDGSI
                     TQSISLDRQLNIIQVADLVLPDAETTFEEYTTMELLSVSMLIVISTFALDFIHSNREI
                     FKIVDLDEAWSFLQVAQGKTLSNKLVRAGRAMNAGVYFVTQNSDDLLDERLKNNIGLK
                     FAFRSTDIHEIKKTLEFFGLDREDESNQKRLRDLENGQCLMQDLYGRVGVVQVHPVFE
                     ELFHAFDTRPPVQERME"
     gene            complement(12541..12930)
                     /locus_tag="IWO89_002221"
     CDS             complement(12541..12930)
                     /locus_tag="IWO89_002221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HAQ0456940.1"
                     /translation="MKKIKSYTSIWAVEKVIYAINDFQLPFPVTFNQMAWFVLSLLFV
                     IVFAHVPPLSMIEGAFLKYLGIPVAVTWFMSQKTFDGKKPLGFLRSFISYHLRFKVTF
                     AGKKVKEQKKRWDEPITLVRSVNYVPD"
     gene            complement(12990..13493)
                     /locus_tag="IWO89_002222"
     CDS             complement(12990..13493)
                     /locus_tag="IWO89_002222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010718210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein ArdA"
                     /protein_id="HAQ0456941.1"
                     /translation="MEQMRVYIANLGKYNEGELVGAWFTPPVDFDEVKERIGLNDEYE
                     EYAIHDYELPFEIDEYTPIEEINRLCGLAEELEGTPVGEVASEIQHAFFNSFEEMVEH
                     VDDIIYYPDCDDMSDVAYFLIVEAGDFGEVPEHLKTYIDFDACGRDLEIGGNYLVTSR
                     GVFEYPY"
     gene            complement(13506..>13682)
                     /locus_tag="IWO89_002223"
     CDS             complement(13506..>13682)
                     /locus_tag="IWO89_002223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002570387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0456942.1"
                     /translation="QSLVLLAIVVIGLFLGFKREFSKLIGFLVIALIAVGLVFNASGV
                     KDVLLSLFNRIVGA"
BASE COUNT         4203 a         2699 c         2501 g         4279 t
ORIGIN      
        1 tgaaaaaatc tttaaaactt gcatcagcag ttatattaat gtcccaactc cttatagcaa
       61 cacctttcca tgtgttggca agcgaaaatt caacatacaa aattgcaaaa gaaagaaccg
      121 agacgactga aatgagtaat tccgatgtaa cagcagagag tagtgaactt ttgacaactt
      181 catcaagtga aagtagtacg acttcaaatg ccgagtcttc aacaacttca tcaagtgaaa
      241 gtagtacgac ttcaaatgcc gagtcttcaa caacttcgtc aagtgaaagt agtacgattt
      301 caagtaccga atcttcaaca acttcaagta gtacaagtga aataacttct tcgaaagtga
      361 caaaaccaaa ccatatagaa tcttcaaaaa aggaagcgcc aaaaacggaa ggacaaacaa
      421 aaactttttc gacaccacag caaatagaat caacagaatc tttccaatca caaccaatac
      481 tatctggtaa tgaagaaatt cattttgaca aaaatgaatc tgtagaaaat tttattatga
      541 aaattggtga gtctgcgaga aaaattggtc agaaatatga tttatatgct tctgttatga
      601 ttgcacaagc gatcttagaa tctgccagtg gtcaaagcca gttagctcag gcaccgaatt
      661 acaatttatt tgggataaaa ggaacttata atggaaactt tgtcattatg gtaacaaacg
      721 aagatttggg taatggaacg ctttatacga cccaatcaaa atttagagtg tatgaaaact
      781 atgaggaaag ctttgaagat tatgccaaat tattaactaa gggaattagt ggaaataaag
      841 atttttatgc aggagcatta aaagctaata gtaaaacata tcgagaagca actaaattcc
      901 ttacaggacg gtatgcaaca gatacacaat actatttgaa gctaaatgaa ttaattaaaa
      961 cctacgattt aaccaactat gataaagaag ttaaaggtcc acaaatgagt aagactggat
     1021 accaagtgcc tattcataat tattcaataa ccagtttgtt tggaaatcgt ggaaatgaat
     1081 ttcaccgagg acttgatatg gcagctacac agggtgagcc gattcatgca gttaaagctg
     1141 gtaaggttgt taaagcagag tttcactact cttggggaaa ttacgtggtg atacaacacg
     1201 aagatggatc aaccgcgtta tatgctcacc agcaacaata cctagtaaaa actggagata
     1261 atgttttgca aggacaaata attggctatg taggaagtac tgggaatagt actggcccac
     1321 accttcattt ggaaatttgc aaagatagta gtctttctca gagcatgtta gttgatccga
     1381 aaaccttatt attcggagac taaaaaaggt aatcaatctg acaaagtaac aagaaaatgg
     1441 ttggattctt agacctttga tcaggatatt gagactggaa ttgatatctc gattacgatg
     1501 ggtttgacaa ataaaacaag tccattatcg aatatcgagt gtattattgc cgtctttatg
     1561 ctcatatttt gagcaggttt cactagatga aaacaattta tctatcttga ggatttcgtg
     1621 tacgtatcgt tcagtcttgt attgtaaatt agtcataaaa tcagactaag aaaaattgga
     1681 aatatttttt tctgatttat taaaagttaa ttgaaattat atggtacttc tttcttggga
     1741 gggtaggtgc agtgaaggaa agatatggtc accttactgt tgttgaggag attactcata
     1801 atggatataa aaaatatcgt tgcctatgtg actgtgggaa tgaaacgatt gtatatcgat
     1861 caaacttact atcaggtaga accaaaagtt gtggatgtgg aaattataaa aataggcata
     1921 aatataaaaa tttgacaaat aaaacttttg gtaagttagt tgcaatcaag ccaactaaac
     1981 tgcgaagtga aggaactgtt gtttgggaat gtcaatgtct gtgtgggaga aaaaagttag
     2041 tatcggcaaa gcgacttcta aaaggtgatg taaaaagctg tggatgtttg aaatcaaaaa
     2101 agcatcacat tgaaaataaa aaattcggat tacttactgc aaagtatcct ttggacaata
     2161 actgttcatg gaaaacaaaa tgggtttgtc attgtgagtg tggcagtatg tgtgagataa
     2221 gttatagtaa cttatttttt ggacatactc aaagttgtgg atgtttaaaa aggcaagaaa
     2281 caaggacatg tgttgaaggg acagtaatag agtgtctaac gtcaaaatta ccgaaaaata
     2341 atacaagtgg tgttaaggga gtttatcgtt ctaaagagaa atagattgcg tatataactt
     2401 taaaaaaaaa gaggtactat ttaggatcgt atgattctat taagagtgca gctcaagcta
     2461 gaaaagatgc tgaaaataaa ttgtttcaac ctttgattga caaatatcga ggtaatcatt
     2521 aattattgtg gataataatt aacagtgata tataaacatc tataatatat tattcatact
     2581 attgatattt agtataatcc tatggaattg aatatcatag caatgtattt gaaaagcaaa
     2641 ttggaaaaca aataggtgca acaccataaa tatgaaaaaa ttgggaaaaa gtagaatgga
     2701 aatttgctta cgtctcacga gatagctcac actattaatc tgaagaacaa tgaagtcgac
     2761 ttttagaaat tgctagaaga aagtaaagcg aattatcact agttactata gagtaagttc
     2821 aaataaagca tctccaactt acatggttac atgaggatat caaatcgaaa ttactcaagc
     2881 ttcctatggc tagagtcgcg agaagttgtt gggcagtaga cataggttat attgttctta
     2941 gctacacgat cattagaaag gaaatcctat tatgtatagt tatcaagaag atgcaaaagt
     3001 ccaaaaaaat cgctggtgga ttttgattgc tatatctatg tttaccttca tgtccacatt
     3061 ggatggcagt attgtcaata ttgcactacc taccatctca gatgatatgg gcataccaat
     3121 gaatcaagcg gaatgggtag tgtccgttta tctgatgatt gtctgttctt gtttgttgct
     3181 gtttggtaaa attggcgata gccgtgggaa aatcaaagta tttcggattg gtacgctgat
     3241 ctttgtcttg ggatcgctgt tgtgtgggtt caatcattcg ctaggtattt tattgttcgc
     3301 acgggtggta caagccattg gtgctagtat gactatggct acaaatgccg gaatcatcac
     3361 ggaagtcttt cccatacatg aacgtggtcg ggcattggga gcaataggtg cgtttgtttc
     3421 attaggctcg attgctggac caggtcttgg cggcttgatt ttgtcccaat tttcttgggc
     3481 gtacattttt tggatcaatg taccggtggg aatcgtaacg atgttgattg ctgaaaaatt
     3541 cttaccaaaa gatatcacca tgagcggcca tacaatcgat ggagggggct ttgctgccat
     3601 tgcggctacg attatcagct tttttggagc aatctttttg gggcaagaaa gtggcttttt
     3661 ccatccagtc ccccttgtac tgttcgtaat ttcagtgatt gcctttgtca gctttttaaa
     3721 agttgaaaag aaagcagcct ctccattgat ttctttcggt atttttaaga acaagatttt
     3781 taccatgagc ttgattacag ccttgttgat tttttcttcc aacttctttg tcaatgtagt
     3841 gattcctttt tatctgcaaa atgctcgcgg gttgagtcct agctatgctg ggctgttgat
     3901 gatggtcttt cctttgatga tggtcgtcgg ggcgccttta agcggctacc tgaccgaccg
     3961 ggttggcccg gagattttgg tattagtagg cttgggctta ttgtcggtaa cccaggtcat
     4021 gtatatgttt ttgtcagtgg actctccttt gtggctatac attgtcgcta ctgccatcat
     4081 gggtgccggc aatgccttgt tccagtcccc caataatacg atggttatga gctcggtcac
     4141 tatcgaaaac ttgggactgg caggctcgat gaattccttt gcccgaaatt tggggatggt
     4201 cagcgggatt tcgctttcta cgacggtctt atatcaagcg atgtctgcta agatgggcca
     4261 gcaagtaact aactatatcg caaatcggcc ggatgtcttt ttatatggaa tgaagattac
     4321 ctttttaggc agctttgttt tgtgttttgt tgcctttttc ctgactatct ggcgtatcaa
     4381 aaaacaagga ggtattcaga aaaatgtctg aaaaaaccac tgttgaagaa tttttggagg
     4441 cctgcgtatg ggtgagtggg atttccatgg atgatggaag atgaagtgaa cttctaaaag
     4501 ccgaaaaact caagtattat cttcaaaata ctagtgctca atgagatgct tgatttgatc
     4561 acaagaagac tgcgaaagcc gctggcaacc gcaagctagg tatcccaccg atggactacg
     4621 ttttagaggc tactggttat gaatacggag ccaacgctct catctgcata agaatacatc
     4681 acccagatta tgtaaagatt ttcgatactt ctgtcaaaaa atcattaaga tagataaatg
     4741 atttccgtct agtcaaattg gttcagaatg tagacacaca gatggtaaga agtcactcac
     4801 aattcgagaa tggacttgtc ctatttgccg tatacatcat aatcgaaacg tcaacgatag
     4861 tatcaatatt ttgaccaagg gctatgaata caagcattgc cttagaaaac atcaagaacc
     4921 ttaggaacaa cggggatagc ttggttaata agagataccg ctgtttaggc ttgcaagtat
     4981 actcttttat caagaattcc gtggctttag tcatgagagg cttaatacta tgaaaatttt
     5041 ctttagcatt tttgatttag gttccttttc taattataat aagatgacca ctacagtaac
     5101 ctactgaaat atataaacag ccactgtagt accattttca aagtagtcta tgagaggaga
     5161 atcttcattc ttatgaacgc tggaattgcg gatatgaatt taataaaaaa aacccttaat
     5221 gattttactt ccaattcaat atcaaaaggt actggtatca atttaagcac aataaaaaag
     5281 ctgaaaagtg gtgaaagatc agtggaaaag ctgaatcttt tagatgctat aaaaataacg
     5341 gaatttgcta tgaaaaatgg aaaagctgag attgaaatat ggagataatt atcgtctagt
     5401 gaattacttg ccacttagct gaatttaacg atccttggtg cttgaagggg gagattcttg
     5461 ggaatagagc atacttgcaa gcctatgtct ttactaacaa cgatatctct tattaaccaa
     5521 gctatccccg tagttctaag gcttttactt ttatctaagc aataatcgta ttcaaaggaa
     5581 atttttgacc cttagaaagt ctcattttat cagacgtgag cttaggcatt gctgaatttt
     5641 gttaattcgg ttttaggttg tgctttgagc ataatatgaa agtattcttt atcacgattt
     5701 cattcatcta aggtaatgtg atgtgattct gaaattcttt caaaggcagt ttttgcataa
     5761 gctgacatta catcatcaat gacatgttta caatgtttca ctactaaaat aagggggtaa
     5821 ccaagaagaa aaactgaatg attatttgta tctaattcca tggtatatca accatttcct
     5881 atatagactg attatgacat ggattaaaat aaaaaggcac tatgtcttaa ttgtcggttt
     5941 tcgagttacc gaatggcatt catcacccac ctatagagga tgggcgactt ctgcctgaaa
     6001 taagttacac tacttataac ttctaataaa attagattga gtcattatta tttaacatca
     6061 acgattttcc agtttccatt tttttgtaat actaatttgt actgaaagta gctagtggtt
     6121 tttgatacat tatcaaggta ttttatcttt gtactcacta gaaaattttt tccgttttgt
     6181 agataaactg gattctctaa ctccgaaaac tttaagtcaa tattgattgg tcgcatcgca
     6241 ccattttcaa cataatactc caattctttt tcagaagctg atggataaag tttaaaaaat
     6301 gtttctaaaa aatcggtgat ttcttccgtt gttttggcat caatttctga atcgttttct
     6361 acttgttttg cttcatattt tgccttgtct ggctttccaa tcattgttgg caaactagtt
     6421 actaaatgat tcctgttttc atcttcaaag attgtcactc gatatgcaga agaaattgat
     6481 tgcgtctttt ttccctctgt aatatgttgc tccactaaaa atgaaacgac aaattcttcc
     6541 gagtgttttt caacatctag aatttttaca gattgaactt cggaactcgt gggaatatct
     6601 gctcgaacca tatcttggat taaagcgaga ccgttttctg taaggtactg ttcgagatta
     6661 ctcattctct tttcaattgc ttctttgtta tttttccatg aaaaatattc tttcgcaaaa
     6721 tcctctgtaa aactttcaac gccagaagta ttcactacct tctcttttat aaccactttt
     6781 tcatgagttg tatgttgatc aatggcggtg aaatttttat aaacgccaaa agcaagacta
     6841 caagacaata aaagccaaag ggcaagcacc atttttcgat gcttacccac ggatacaact
     6901 cgttcttttt tcttttttgg gctcttttcc ttttgattcc gttcaatttt tatcttcatt
     6961 ctttttcctc ttttctctta tttgtatcgg ccagctccga taagatgctg ccgccaatag
     7021 ttttccgtta gattcgtcca ccccactgga tctcctgcat gaaacatgcg attctcccct
     7081 gcgtaaatgc ccacatgggt cacataagta ccggcatcat aggttgaatg aaagaaaact
     7141 aaatcacctg gtttggcatc ttttagatca atatgagtca tggcatcata ttgatcctga
     7201 gccacccgag gaagtttcag gtcaactttg gcataacacc attgggtcaa gccactacaa
     7261 tcaaaccccg tttctggact tgagccacca aacacatagg ccgttccttc atacttaaat
     7321 gcctcatcaa aaatttcttg gactattttg tcatcaaact gagttgtagt taggtattgc
     7381 ttcactaata aacagtaaaa catattgcca taattatacc gccacccacc attttcctta
     7441 atcgcgattg ggttggggta atcgactttt tgaccacctg atttttcctt tgaaaaagat
     7501 tcagctaaag caaaactatg cttcttcccg tttttcccga catgatctag atacccgcca
     7561 ccatagttat aggcctgaat aatactatct tgatcacacc ctagttcttt ggaacgctta
     7621 accaaagacg caaaatagag acagccttgt ttgatggatg cctcggtact taatgaatta
     7681 ggtggtaatc cggctgactc agaactttgc ataacgtctt ccattgtgcc accagattcc
     7741 acttgaataa tcgccaaaag aattggaata tactctgtta tatccgattc tttggcatac
     7801 ttttccacca ttgcacggtg tttcaatact tcttctgaga cactagttcc gccaggagaa
     7861 gtgccagaac taccatttcc ctcactgtct tcaccaaaca acaaacccac acacatcaaa
     7921 atacccataa aagagatggt cagaaaagtt aatgaaataa cacccacttt cttcaagttc
     7981 attttttctc acctgacctt gattttggac gatggttcat tcttttttgt actttttgat
     8041 tggtctgctt tggcagagtg gacctctgtt tcacttgttg gaaagaattt ttcttacttg
     8101 ctgattggct tttccctttt gaaatgattt cttgactagt tagttttcgt ttgatttcgg
     8161 gattcagatt tgctctcaca atccgctttg gtgcttcttt tttcagcatc atatctttct
     8221 gaggttgagc tgctttatct attggaatat tcgcccttct ttttccctga cgatttgttg
     8281 cttcactcag tatcttgcgt ttttcgtcca ttttcgcatt tcttcgttgt cgttgttttt
     8341 ctcgcaaatc cactcgatcc gctttttctt ggaacagtcc gcgcttgaat tcttctggtg
     8401 cttttttcga cttttcgatt cctttatgga gattgtattt caaattcgtt ggcgtattcc
     8461 ttacctgatc ctttacttgt ttagtcttat ctttcaccaa agcttgggta tccaaaactt
     8521 tgccgatttt tctcccagcc aaattcattc gggattgttt cttattattt gaaactggat
     8581 tctccttagt taagtcagat agtatttcgt gatcgttcgg taatcgtgaa ttctttcgta
     8641 gagaagaacc ggatggctta aatttggatt ttgattgttc ctttgccaac ttatttccga
     8701 ctgctgcacc accaccaaaa gccaaagctc gtcccacatt tcgattgagt tgccgcaatt
     8761 ttcgattcat taacatttgc ggttttcgca tgacgcgtct tcccattgac tggctatcat
     8821 tactctggag gttgaacata ctcataatgt cgcccaattt gaaatagata ccggcaaagg
     8881 tcacaatttg cagaaaagca accatgaaaa atggataggt tgccgaaata ttgaaaaaca
     8941 tcgtggagat gctaaatgca gtcgtaatca ccaaagtaac acccgctcgc atcataatcg
     9001 tattgaataa acgaataatc gccttctttc ctaaactttc ataagtcggc aacatggaca
     9061 ataagaaact aattggcaga aacatcgcaa agataatgaa gagaatttgc gagaacaaca
     9121 taatccctgt tagaagaaaa acaaagaagg aaatgccaat attgaacaat accaaaaaga
     9181 ccacggtccc taaacggttc atggtcttgg taatactgag attcgcattg tcattgtctt
     9241 caatctctgc cttgaccgct tcttcccgat ctttgccttt gttttcatct ggactcacag
     9301 atagaatctt gttgacccgg tcttcaccaa tttcttctat gttggaatca tcaaattgca
     9361 gtagtaacca aggttgttgg acttgaatcg aaaacaaact atcccgaatc aaatccacac
     9421 tatcttttcc ttggctttct gaattgggta caacgatctc ggttccaagg gtaagggccg
     9481 cttcactaac atctgagcta aagtcattga tttttgtgat ataggtaggc gcataagcaa
     9541 tgaacgaacc cgatagtaga aaaatcatca caaaattaat cacggctcga actgctttcg
     9601 tggtttctcg ttttaataat ccggtatacg ccacatacac ccccagagct aaaatcatca
     9661 ataatagaaa cccaaaataa aatccggagg tctggagtcc attttcagta ataccagcca
     9721 atgtttggat attctttcca atactttcag ccgtatccga aatgaaatct aatttgtaag
     9781 cctcttgaac cacataaccc gtggcgttcg acaaatacaa actcaccgtc caaatgaaat
     9841 tagtaatggc ataaagacca tacatcacgc ttttccctaa gccatcaccc caattccaag
     9901 gcaaccaatc ccaagagcta tccacaaaga aatctagttg atagttgttc agtgaatatt
     9961 tggaatatag atttcctgct tcaactgtat catcaactag ccctgtcgcc tcggctaccg
    10021 taccaactaa actcaataga actatcagag tgagtatgct aataaaaacg attcccacaa
    10081 tccgaaaaag tttctttttc atcccctcac tccattctct cttgaacagg tggacgggta
    10141 tcaaaggcat ggaagagttc ttcaaagact ggatgtacct gaaccacgcc tacccgacca
    10201 tacaaatcct gcatcaagca ttggccattt tccaaatctc gtaggcgttt ttgattgctt
    10261 tcatcttctc gatccaagcc aaagaattcc aaggttttct tgatttcatg aatatcggta
    10321 ctgcgaaacg caaacttcaa cccgatatta ttcttcaagc gttcatccag taaatcatca
    10381 ctattttgcg tgacaaagta aaccccggca ttcatagccc gcccagcacg aacgagctta
    10441 ttcgataatg ttttcccttg cgctacttga agaaaactcc acgcctcatc taagtctaca
    10501 attttgaaga tttctcgatt actatggata aagtccaagg caaaagtgga aatgacgatt
    10561 agcatggaaa cactaagcaa ttccatggtg gtatattctt caaaagtggt ttctgcatct
    10621 ggtaaaacca aatccgctac ttgaatgata tttaactgcc tatctaaact aatcgactgg
    10681 gtaatcgaac catctgagaa aagcaaatgg gcaaaatcat aatcggtaaa ggactcgatg
    10741 tgatccgcga tattttccgc aactggtgag ccttcttttc gaagctcatt aattacttgt
    10801 aataatcccc gttgattgct ttgggtaacc gtccgaatgg ccttacgcaa cactggaaac
    10861 ttctccccat ctcgactaga aattcctgtt aaaaaggtca gaatatcaat tgctagactt
    10921 tcagaatcct tctttttctc cataatcaca taaggatcaa gtaatccttg attttttggc
    10981 ttactagtta agttgagaat attgatttca tccgcaattt ctggcaaggt ttctttccag
    11041 ccaccccgtt ccccttttgg atcgacaatc acagcttgtc caccaaaaag gacggcgtaa
    11101 taaactaaga gattgttact aaaggacttt ccgccaccga gtgaaccgag gaatgccgct
    11161 gctagcgcat tggtaactga atccttgacc ccttgtgagg ccaatgccgg tttcaggtaa
    11221 acattgcgcc ccgtatctac gttataacca aagtaaatgc cttccaactc ccctaacatc
    11281 tgagtggcgc caaaaccaag tccggcgaga aaatcagacg taacatactg gatataatca
    11341 ttcatgtatc gcttactaga aggtaagaat tcttcatgta gccccatcat atcgccaaaa
    11401 ggccggacta gttttacatt ggtatcatca taaaaatcta gcacttcatc acaccggcgc
    11461 tttagttcat ctactgattc ggcacttact cgaaccacat agctcagttt gtacatggat
    11521 tctttggact gatccaaggt agtttccaat tcatctacgg aatccaatgc atctaagaca
    11581 ttggaatttg tttcattgtc agattgatag gcatggttgt ccaagtcttt taattccttt
    11641 ttcttattgc gaacggtagc aagtgccttt ttgttcgtca caatctccac attcatacta
    11701 gtgtcaattg ggaatgtaaa ttgttgttgc tggtaataga aaagttctga ggagggaaac
    11761 tccatttccc ccacaattgt attaattgtt aagtacgcta catatgattc atgattctca
    11821 tgttccatgc ataagtaccg gggcttttct tcaatcaaac aacgactggg acgtaataag
    11881 tcgtaacgtt tcaccaaagt ttccgacttc agctttttct ttggtagctg aaattcatat
    11941 tcttcaaatg gtgtaccctt ttcgccatag atatgttcaa tcaaatacat cagatcactc
    12001 ggtgtgactc gtctgacttt aaatcttcga gccaatttac tttccattag tttttcaagc
    12061 ttggtatatc ggcgaatttc ttcattggat aaggaaacaa aatcgcccat caaatgatga
    12121 ttcactccat agacaaattc ttgaaagccg gagaagaaag atttcttgag attctttaga
    12181 ttgacctctt catctgtcgc aatcagtttg aagccaataa aaaagcgata atcaatctga
    12241 ctatcgccaa tcatggatac taatgcctct gtctgtaaat caattcgttg tttcgctact
    12301 tctgttaatc gcccggtaat ttcctttttt gatcgttctt gcgtggcacg aatactactt
    12361 tctgtggcaa tttgtaaggc atgaatcttg ccttcacgat tttgcgcaat caattgacga
    12421 aagttatcgt ggacttgata tttctgttca ggtgacaaaa aggagtaatt gtaaggtatt
    12481 aactcatagt aggcaaaaca ttctccctct tgattaaaga caagattatt ttcaatatac
    12541 ttaatcgggt acatagttca cactccttac cagagtaatg ggttcgtccc atcgtttctt
    12601 ctgttctttg actttctttc ctgcgaaagt gaccttaaat cgtaagtgat agctaataaa
    12661 agatcgtaaa aatcccaatg gtttcttacc gtcaaacgtt ttttgcgaca tgaaccaagt
    12721 aactgccacc ggtataccta agtattttaa aaaggccccc tctatcatag atagtggagg
    12781 cacatgggca aatacgatga caaacagcaa ggataaaaca aaccaagcca tctggttaaa
    12841 agtcacagga aaaggcagtt gaaagtcatt aattgcataa atgacctttt caactgccca
    12901 aatactggtg tagcttttta ttttcttcat caaatcattc ctttaaaaag aaggatagtt
    12961 tagaaatcaa ctacccttca ataattttgt taatatggat actcaaaaac acctcgactc
    13021 gttaccaaat aattgcctcc aatctccaag tctctaccac acgcatcaaa atcaatatac
    13081 gtcttcaagt gttctggtac ttctccaaaa tcaccagctt ctacaatcaa gaaatatgct
    13141 acatcactca tatcatcgca atctggataa tagataatat catccacatg ctctaccatc
    13201 tcctcaaaag aattgaagaa agcatgttga atctcagaag caacttcacc aactggcgtt
    13261 ccttcaagtt cctccgctaa gccacacaac cgattaattt cctcaattgg cgtatactca
    13321 tcaatctcaa agggaagttc ataatcatga attgcatatt cctcgtattc gtcatttaac
    13381 ccaattcgtt ctttcacttc atcaaaatcg acaggtgggg taaaccacgc ccccaccaat
    13441 tcaccttcgt tatatttccc gaggttcgca atataaacac gcatctgttc catcgtgctt
    13501 cccccttacg caccgacaat tcggttaaac aaactgagta agacatcctt caccccagaa
    13561 gcattaaaga ccaatccgac cgcaatcaaa gcaatcacca aaaagccaat cagtttggaa
    13621 aactcccgct taaatcctaa aaacaaacca atgaccacaa ttgccagtaa gactaaggat
    13681 tg
//
LOCUS       DACEEZ010000077        13429 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20313_2017
            SAMN12639966-rid9879223.denovo.077, whole genome shotgun sequence.
ACCESSION   DACEEZ010000077 DACEEZ010000000
VERSION     DACEEZ010000077.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639966
            Sequence Read Archive: SRR10023158
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13429)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13429)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741683.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741683.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:44:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,016
            CDSs (total)                      :: 2,962
            Genes (coding)                    :: 2,889
            CDSs (with protein)               :: 2,889
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 36 of 73
            Pseudo Genes (incomplete)         :: 29 of 73
            Pseudo Genes (internal stop)      :: 18 of 73
            Pseudo Genes (multiple problems)  :: 8 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13429
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639966-rid9879223.denovo.077"
                     /isolate="E20313_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(<1..914)
                     /locus_tag="IWO89_002224"
     CDS             complement(<1..914)
                     /locus_tag="IWO89_002224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase family protein"
                     /protein_id="HAQ0456943.1"
                     /translation="MIISTITEAIGSTPLYRFDNQHYSMPKGSAIYAKLEYLNPGGSI
                     KDRLGSYLIKEGFAQGKINEKTTIIEPTAGNTGIGIALAALTYKLRTVFVVPEKFSLE
                     KQQLMKALGAKIIHSPSEQGIIGAISKSKKLAEEISNSYLPLQFENKDNPAAYYHTLG
                     PEIFHEIKENIHSFVAGIGSGGTFAGTSTFLKEKYPDIRIIGVEPEGSVLNGGDPAPH
                     EIEGIGVEFIPPFLSPLSINQIETISDVEGFNYTRQLAREQGLLVGSSSGAAFAAALR
                     EIRRLPPGHRVVTIFPDAADRYLSKNIY"
     gene            1342..1905
                     /gene="efp"
                     /locus_tag="IWO89_002225"
     CDS             1342..1905
                     /gene="efp"
                     /locus_tag="IWO89_002225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HAQ0456944.1"
                     /translation="MISASDLRAGMTFVQDGKLIKVLDASHHKPGKGNTVMRMKLKDV
                     RSGATYDTTFRPEEKFEKAHIDTKPVQYLYTMDDTAFFMDLETYEQYEIPVETVEEEM
                     KYILENMEVKIQFFGSEVIGVQLPTTVVLRVVETQPSIKGATVTGSGKPATMETGLVV
                     NVPDFIETDELLEINTAEGSYVKRASK"
     gene            2391..3650
                     /gene="lysA"
                     /locus_tag="IWO89_002226"
     CDS             2391..3650
                     /gene="lysA"
                     /locus_tag="IWO89_002226"
                     /EC_number="4.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HAQ0456945.1"
                     /translation="MTELIIGGVTASTLAEKYKTPLYVYDEEKMRQTMLSFKRGFVSK
                     RFDTKVLYASKAFQTIEMLHLVREYGFGLDVVSGGEIYTALQADFPRDTIYFHGNNKT
                     PEELIYALENDLLHIVADNLMEVELLAELSQRYLKKICIMLRLNVGVEAHTHEYIVTA
                     HIDSKFGMSYESKECQQALQLIKESSYLELEGFHAHIGSQIFDVTAWLAEIDKLTNYL
                     TDFDQPLSLNLGGGFGIRYTQNDQPIPVEEALKKLIEYTEEALEKRKLTIRQLLIEPG
                     RSIVGEAGTTLYTIGFIKKTPHKKYYFVDGGMTDNIRPALYQAEYDCDLAAKLSEEKT
                     EKVTIAGKMCESGDVVIKEAYLPKAESGDLLAVYSTGAYGYSMSSNYNRATRPAVVFV
                     NNGQSRLIVRRQAFSDLLRGEVSYDPS"
     gene            3637..4437
                     /gene="dapF"
                     /locus_tag="IWO89_002227"
     CDS             3637..4437
                     /gene="dapF"
                     /locus_tag="IWO89_002227"
                     /EC_number="5.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate epimerase"
                     /protein_id="HAQ0456946.1"
                     /translation="MILHKYNGCGNDFILLDYAEDTDYSQLAASLCTKEKYDTDGLIA
                     VKIDPLEMIYYNKDGSRAPMCGNGIRCFARYVNDQNMMNEQEFNVETLAGTRKITVLD
                     PSPFYCSVDMGVPDDSPEKIKANQTYPIKNQEIEINGEKVTITSLFLGTIHTVVFVEN
                     AREEVWRKRGELICHHPLFKEKTNVNFVEVVNEQELIVRTYERGVGWTLACGTGCCAS
                     YVVAKETGKISADQVVVHLEQGDLMISGEDAIQMAGPAVHEWTRILEE"
     gene            4440..5321
                     /locus_tag="IWO89_002228"
     CDS             4440..5321
                     /locus_tag="IWO89_002228"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HAQ0456947.1"
                     /translation="MIKGSIVALITPMNEEGSVDYAGLEKLIQFHLDEQTDGLLVLGT
                     TGESSTLTQSEEEQILQLTVKKVAGRVPVIAGAGTNNTKETIEKAKHFASLGADALLV
                     ITPYYNKTSDAGLAAHFTAIAEASPIPLILYNVPSRTGMSIPIHVLVNLAEHPNIIGL
                     KEASGDMAYVMDAARLIGEEFFLYSGNDDLILPVMSVGGSGVISVWANIQPKIVHELV
                     KDTQDGRWQQAKEKQLNALELIHALFSETNPIPVKAAMSLLDLPSGPLRLPLVSLSKE
                     KKKQLAQLLLKERTVEK"
     gene            5318..6034
                     /locus_tag="IWO89_002229"
     CDS             5318..6034
                     /locus_tag="IWO89_002229"
                     /EC_number="1.17.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate reductase"
                     /protein_id="HAQ0456948.1"
                     /translation="MKIGLIGSGQMGNRVEEVAQERGDTVLLLRTAPKEATTLAFTEL
                     PELLIDFSHPDNLEKILSYSLSYQLPLVIGTTGYTQSQFQEIKEHAKHVPVMYSANFS
                     FGIMVMNQLIKQAAAMLQGWQIELIEKHHSRKKDAPSGTANMLLQTIQEVQKLQPVYN
                     WQGKKREENQIGVHSIRAGSLPGEHDVLFAATDEIFTIKHESFSNRIFAAGAVEAADW
                     LKDQSPGYYKLEDLLMDKGV"
     gene            6040..6735
                     /gene="dapD"
                     /locus_tag="IWO89_002230"
     CDS             6040..6735
                     /gene="dapD"
                     /locus_tag="IWO89_002230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-acetyltransferase"
                     /protein_id="HAQ0456949.1"
                     /translation="MEAQEIIRYIQTASKKTPVKVYLNVTEPIEFKESKVFGEGSSFV
                     VFGDYETIAPVLEAESEKIIEIEIETAARYSAVPLLDIKKINARIEPGAIIRDQVSIG
                     NNAVIMMGAIINIGAVIGENTMIDMGAVLGGRATVGKNCHIGAGAVLAGVIEPASAKP
                     VIVEDGVLVGANAVIVEGVHIGKDAVVAAGAVVLEDVAAETVVGGIPARVLKISDDQT
                     KENTALIAALREL"
     gene            6751..7752
                     /locus_tag="IWO89_002231"
     CDS             6751..7752
                     /locus_tag="IWO89_002231"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HAQ0456950.1"
                     /translation="MREIDTCIVGATGLVGQTMLKVLEEYDFPVGRLKLLASKRSAGK
                     VCVFKKKKHTVQELTETSFEGYDLALFSAGASVSKEFAPIARSQGLFVIDNSSAWRED
                     SDIPLIVPEVNLEDYTLNRLIANPNCSTIQAVLPLKALQEAFGIQRVNYSTYQAVSGS
                     GQKGIEDLHRTQNDEEPVLYPYNISQTVIPEIDQPLENGYTKEEQKMINETRKILHQP
                     ALPVSATCVRVPIENGHGVSVAVQLQKEFTTEEIRECLSQFPGIQLEDDLAAHKYPTS
                     ILARGTDMVYVGRIRKDTSLENGLLFYTTADNIRKGAAANAVQIAAALMQKIAEVAR"
     gene            7749..9110
                     /locus_tag="IWO89_002232"
     CDS             7749..9110
                     /locus_tag="IWO89_002232"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate kinase"
                     /protein_id="HAQ0456951.1"
                     /translation="MSNTLKVAKFGGTSMSHEEQFKKVRTIVLSDPNRQVIVVSALGK
                     RTSDDDKVTDLLYLIYAYLQHHVDWTPLWKRICDRFIQVRDVLALQLPIEDELARIQK
                     QLEDEKITEDYLVSRGEYLTAQLMAEYLGYQFIDAKDLIFFDYDGKIDQEKTAQEITK
                     AFKRYPKMVVPGFYGANPAGKEKLLGRGGSDITGAILAKMLQAESYENWTDVSGIMMA
                     DPRIIDHPKIIEEISFRELREMAYMGANVLHEAAVFPVQEADIPIQIKNTNDPFAQGT
                     KISNHEIEKENGLTGIAGRKDFLSITLFKRHMSDEIGFIWKAMSIFAKHEISIEHIPS
                     GIDNIGVIVSAEAIADKLFLITKELKETLGVEEIVVIEDLALISVVGGPHKELIGLSG
                     KVLSILNKLEIRTSILSQGAQELNLIIGVPNNQYETVVKGIYEGMVNTDAERISNGTP
                     SNS"
     gene            9070..10221
                     /locus_tag="IWO89_002233"
     CDS             9070..10221
                     /locus_tag="IWO89_002233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HAQ0456952.1"
                     /translation="MLKEYRMALHQIPEIGFHEFKTKKYLYEQVKNSGAVIHEIGETG
                     LLLYFDNQQEETIAFRTDIDALPVKEETNLSFASKHEGYMHACGHDGHMAMLLGLTDF
                     ITKHIEDLIYNVVLIYQPSEEISGGAQSVIQSGLLDKYEVQAIFGFHLWPGLAKGEVF
                     SRPGPLMAQSSETDIIIKGQSAHIASSEKGIDSLEAAVRFLKNVYDFDRQLPAEWNHL
                     MKFGQIQGGTIRNVLAGEVIVSGSIRSYSSDMQNYLKRGLDHLAEQFHSTATISFRYN
                     DGYPAVINHRGLYAALLDSNKLHELDAPVLQAEDFGIYTQQYPCVFFFLGVGDTPALH
                     DPTFDFDMDVLENGVEWYKTILFTENLLVKIDSSSKEERVNNDEFIQSI"
     gene            10196..11365
                     /locus_tag="IWO89_002234"
     CDS             10196..11365
                     /locus_tag="IWO89_002234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HAQ0456953.1"
                     /translation="MMNLSNRFSPRLSRIEVSKIRQFDQQISSIPDVIKLTLGEPDFP
                     TPEHVKQAGIAAIEEDFSHYTGMRGLEELREAACIFQQQRYGLTYDPQTEVLTTVGAT
                     EAIASALLSVLEEGDKVLIPAPAYSGYQPLVELAGAELIPIDTSDTGFVCQPEQFERA
                     FEQYGSAVKAVILNYPNNPTGTTLSAKQLKAIAEVLKKYPVFVISDEVYAELTYSGTH
                     MSIATYLPEQTIVISGLSKSHAMTGWRVGFIFAQKALIDELIKVHQYLVTAATTMSQK
                     AAAEALINGVKDSENMKEQYQTRRDYLVQQLAPLGFEVTQPNGAFYLFCKLPAAIQTD
                     SWQFCVELAEQAKVACIPGIAFGPEGEGYIRISYASSMEKLHEACKRIKDFLTEQ"
     gene            11540..12919
                     /locus_tag="IWO89_002235"
     CDS             11540..12919
                     /locus_tag="IWO89_002235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HAQ0456954.1"
                     /translation="MEKIKVVIVGASHGGHQSILELLTRYGENVDITLFEAGDYISFM
                     SCGMELYLEDKVTDVNDVRNFRPENFPQSNVHILNNHEVKTIDADKKTVTVTSASDNE
                     TKEYPYDKLILSSGVKPNNLPVPGADLENVYLMRGYNWATKIKEKLNDPAIKKIAVVG
                     AGYIGIEAAEASRKAGKEVVLLDVIDRPLGTYLDAEMTDILEQHLNENGIEVLTNAKI
                     EAFTGNGKVEAIKTSEKEIPADLVIQAAGVKANTEWLKGIVDLDERGWIQTNEYLQTN
                     LPDVYAVGDATLAYSIPARKKMPIALATVARREARYVVKHLFEEVPNKPFGGVVGSSA
                     LSVFDYHFAASGLNSFTAAKAGVDVRVSYYEDTLRPKYVPEEQGNPKVSIQLFSDPMT
                     HQLLGGAVLSTYDVTGQGNVLALAIQNKLTLEELAEADFFFQPGFDRQWSMLNLAAQQ
                     ALGEEPFVE"
     gene            13200..13429
                     /locus_tag="IWO89_002236"
                     /pseudo
     CDS             13200..13429
                     /locus_tag="IWO89_002236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611166.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT         4482 a         2340 c         2926 g         3681 t
ORIGIN      
        1 aagtaaatgt tttttgataa atagcggtct gctgcgtctg gaaaaatcgt aacgacacga
       61 tgaccaggtg gtaaacgacg aatttctctt aatgctgctg caaaagccgc accactcgaa
      121 cttccaacta atagtccttg ttctcgtgct aactgcctag tatagttaaa cccttctaca
      181 tcagagattg tctcgatctg attgattgaa aggggagaaa gaaacggcgg aatgaattct
      241 acaccgatac cctcaatttc gtgaggggct ggatcaccac catttaacac agagccctct
      301 ggttctacac cgattattcg aatgtcggga tatttttctt tcaaaaaggt agaagttcca
      361 gcaaaggttc caccactccc aattcctgct acaaaactat ggatattttc tttgatttca
      421 tgaaagattt ctggccctaa cgtatgatag taagcagcag gattatcttt attttcaaat
      481 tgaagaggca gatagctgtt tgatatctcc tctgcaagtt ttttactttt gctgatggca
      541 ccgatgattc cttgctcact aggagaatgg atgatctttg ctcccaatgc tttcatcaat
      601 tgttgttttt ctaagctaaa cttctctgga acaacaaaaa ccgttcttaa tttgtatgtg
      661 agtgcggcca gagcgatccc aattcctgta tttccggcag ttggttcgat gatcgtcgtt
      721 ttttcattga tttttccttg ggcaaatcct tctttgatca aataactgcc caatctatct
      781 ttgatacttc ctccaggatt taaatattct agttttgcat aaatagcaga acctttaggc
      841 atagaatagt gctgattgtc aaagcgataa agcggtgtgg aaccaatagc ttctgtaatt
      901 gtcgagataa tcatgtgggc ctcctaaaaa aagataataa aaaaaggagc tgactattaa
      961 atcagctcct tgaaagacat agagaaaaat tgagtagata aaaagataag ttacctggta
     1021 ctcattctat gaagggcaat taatagacat gacaaatagt atgctgcttt tatgcaccat
     1081 acaacagcaa cgattcaatt gtttttggtt gctgactgtc atgtgtatcc ctccaagtaa
     1141 aatttttatt aacgatagca tgaaaaaaaa taaaagtcaa aggcttgaaa gaaaaaaata
     1201 tttcgatagc atcgtttacc cgtgtttatt tttctgacaa ataggcatga gaccttaaaa
     1261 gatagagaat actctagttt tctggccggt tgtctgatac aataaagttg attatgataa
     1321 agaaaagaag gagcaaaaat aatgatctca gcaagtgatt taagagccgg tatgactttc
     1381 gtacaagacg gcaaactgat taaagttctg gatgcaagcc accataaacc tggtaaagga
     1441 aatacagtta tgcgtatgaa actgaaagat gtccgttcag gtgcaacata cgacacaacg
     1501 ttcagaccag aagaaaaatt tgaaaaagca catatcgaca caaaacctgt acaatatttg
     1561 tacacgatgg atgatacagc tttcttcatg gatttggaaa catatgagca atatgaaatt
     1621 cctgtagaaa cagtagaaga agaaatgaaa tatatcctag aaaacatgga agtaaaaatc
     1681 caatttttcg gtagcgaagt catcggcgtt caattaccaa caactgtcgt attacgtgtt
     1741 gtggaaacac aaccttccat caaaggtgca acagtcactg gttcaggtaa gccagcgaca
     1801 atggaaactg gcttagtagt caatgttcct gacttcatcg aaacagatga attgttggaa
     1861 atcaatacag cagaaggttc ttatgtaaaa cgtgcgtcaa aataattcgc taacggattg
     1921 ataagaattt gaaaacagag cttgacctct tgaaaggcag atcatcggat ctgcctttca
     1981 agaggttttt tgtggaatca gcaaattgaa aaataaaaaa gtatttgatt ttatttatat
     2041 tttgaaagat gaatggcaaa agctagagaa agaagaaact gcacttattt atgaagaaat
     2101 tgaaatgatt cataaaattg aaaatatccg aactattccg aactatattg aagcgaaaat
     2161 attttttcat cactaaatag tgttgaattt atgtttttat gtataaatag cttctaatag
     2221 ttcgggtatt ttgtttttat aaccattttt attcttattt ttaaaaaaat atttcgaaat
     2281 atactagaca attcagaaaa ttttgtttaa tattattcca ataaaattca aatctttatc
     2341 aaatgaaatt ccaataaaaa cggaaaaaaa gtgaaaagag gaatgaaaaa atgacagaat
     2401 tgataattgg cggagtcaca gcaagcacat tggcagaaaa gtataagaca cctctgtatg
     2461 tgtatgatga agaaaagatg agacaaacga tgctgtcatt caagcgaggc tttgtatcga
     2521 aacgttttga tacaaaagtg ctttacgcct ctaaagcatt ccaaacgatt gagatgctgc
     2581 atttagttcg tgaatatgga ttcgggctag atgttgtcag tggtggggaa atctataccg
     2641 ctttgcaagc tgattttcct agagatacta tctacttcca tggtaacaac aagacgccag
     2701 aggaactgat ctatgcactt gaaaatgatt tgttgcatat tgttgccgat aatttgatgg
     2761 aagttgaatt attagcagaa ttaagccaac gctatttaaa aaaaatctgt atcatgcttc
     2821 ggttgaatgt cggagtagaa gcacatactc atgaatatat cgtcactgcg catattgatt
     2881 caaaattcgg catgagctat gaaagtaaag agtgccaaca agcccttcag ttgataaaag
     2941 aaagcagcta tctagagtta gaaggatttc atgctcatat cgggtcacaa atttttgatg
     3001 ttactgcttg gttagctgaa atcgacaaat tgacgaatta cttaaccgat tttgaccaac
     3061 ctctttcctt gaatcttggc ggagggtttg ggattcgtta tacacaaaat gatcagccga
     3121 tcccagtcga agaggcacta aaaaaactga ttgaatatac ggaagaagct ttggaaaaac
     3181 gaaaactgac cattcgtcaa ttactgatcg aaccgggtag aagtattgtt ggagaagctg
     3241 gtacaacact atacacgatt ggtttcatca aaaagacacc acataaaaaa tattactttg
     3301 tagatggcgg gatgacagat aatatccgtc cagctctgta tcaggcagaa tacgattgtg
     3361 atttagcagc aaaactgtcg gaagaaaaaa cagagaaagt gacgattgca ggaaaaatgt
     3421 gtgaatcagg cgatgtggta attaaagaag cttatttgcc gaaagcagaa tcgggtgatc
     3481 ttttagctgt ttattccacg ggagcttatg gatactcgat gagcagcaac tacaatcgag
     3541 cgacacgacc agcagttgtt tttgtcaata atggccaatc aagactgatt gttcgcagac
     3601 aagcattttc tgatctacta aggggggaag tttcctatga tccttcataa atataatggc
     3661 tgcggaaatg attttatctt attagattac gcagaagaca cagattatag tcaattagcc
     3721 gcttctttat gcacaaaaga aaaatatgat acagacggat tgatcgccgt aaaaatcgat
     3781 ccgttggaaa tgatctatta caacaaagat ggcagccgcg cgccgatgtg cggaaatggc
     3841 attcgctgtt ttgctcgata cgtaaatgat cagaacatga tgaatgaaca ggaattcaac
     3901 gtagagacat tagcaggcac aagaaaaatt acggtgttgg atccctcacc tttttattgc
     3961 agcgtagata tgggagtgcc agatgattct ccggaaaaaa tcaaagctaa tcaaacttat
     4021 ccaataaaaa accaggaaat agagatcaat ggtgaaaaag taacgatcac tagtttattt
     4081 ttaggaacga ttcatacagt tgtttttgtt gaaaatgcgc gtgaggaagt ctggagaaaa
     4141 agaggtgaac tgatctgtca tcatcctttg ttcaaagaaa aaaccaatgt caactttgta
     4201 gaagtagtca atgaacaaga attgatcgtc cgtacctatg aaagaggagt aggttggaca
     4261 cttgcttgcg gtacaggttg ctgtgcctct tatgtggttg ctaaggaaac tgggaaaatt
     4321 tcagcagatc aggtagttgt tcacttggaa caaggagact tgatgatctc tggtgaggat
     4381 gcgatacaaa tggctggccc agcagttcat gaatggacaa gaatattgga ggaatgaaga
     4441 tgataaaagg ttcgatcgtg gcgctgatca cgccgatgaa tgaagaggga tcagtagatt
     4501 atgctggttt ggaaaaattg atccaattcc atttagatga acaaacagat ggattattag
     4561 ttttaggtac aacaggagaa tcttctacgc tcacacaatc tgaggaggag cagatccttc
     4621 aactgacagt aaagaaagtg gctggccgtg tgccggtgat tgcaggagct ggaacgaata
     4681 atacgaaaga aacgattgaa aaagcgaagc attttgcttc tttgggggca gacgcacttt
     4741 tggtcatcac cccttactac aacaaaacaa gtgatgcagg actagctgct cattttacag
     4801 caattgcaga agcctcaccg attccgctta ttttgtataa tgttccttct agaacaggca
     4861 tgtcgattcc aatccatgta ttagtaaatt tagcagaaca tcctaatatc attggtttga
     4921 aagaagcctc aggagatatg gcttatgtga tggatgccgc tcggttgatt ggagaagaat
     4981 tcttccttta cagtggaaat gatgatttga tcctaccagt aatgagtgta ggaggaagcg
     5041 gtgtaataag tgtatgggcg aatatccaac ctaaaatagt ccatgaatta gtaaaagata
     5101 cgcaggacgg aagatggcag caagcaaaag aaaagcaatt aaatgcactt gaactgatcc
     5161 atgccttgtt cagtgagacc aatcctattc ctgtcaaagc agccatgtca ttattagact
     5221 taccttccgg acctctacgc ctgcctttag tttctctttc aaaagagaaa aagaaacaat
     5281 tagctcagct tttgttaaaa gaaaggacgg ttgaaaaatg aaaatcggac tgattggcag
     5341 tggacagatg ggaaatagag tcgaagaagt tgcacaagaa agaggagata cggtccttct
     5401 tttacggacg gcacctaaag aagcaactac gcttgctttt acggaactgc cagaactgct
     5461 tattgatttt tctcatccag ataatttaga gaaaatcctt tcgtatagct tatcatatca
     5521 acttccgtta gtcattggca cgacaggtta tacacaatca cagttccaag aaataaaaga
     5581 acatgccaaa catgtacctg tgatgtatag tgctaatttt tcgttcggca tcatggtgat
     5641 gaatcaacta atcaagcaag cagctgcaat gttgcaaggc tggcagatcg aactgattga
     5701 aaaacatcat agtcgtaaaa aagatgcacc atctggtact gcaaatatgc tgcttcaaac
     5761 aatccaagaa gtgcaaaagc tgcagcccgt atacaactgg caagggaaaa aaagagaaga
     5821 aaaccaaatt ggtgtacaca gtattcgtgc aggttcttta cctggtgaac atgatgtatt
     5881 gtttgctgct actgatgaaa tatttacgat caaacatgaa tctttttcta atcgaatctt
     5941 tgctgcaggc gcagtagaag cagcggactg gttgaaagat cagtcaccag gttattacaa
     6001 attagaagat ttattaatgg acaaaggggt gtaaaaaacg tggaagctca agaaatcatc
     6061 cgctacattc aaacggcatc taaaaagacg cctgtgaaag tctatctgaa tgtaaccgag
     6121 ccaatcgaat ttaaagaaag taaagttttt ggtgaaggaa gcagcttcgt tgtttttggt
     6181 gactatgaga cgattgctcc ggttttagaa gctgaatcag aaaaaatcat tgaaatcgaa
     6241 atcgaaactg ctgcaagata cagcgcagtt cctttgctag atattaaaaa aattaatgct
     6301 cgtatcgaac caggtgctat tatcagagat caagtgtcaa tcggcaacaa tgccgtgatc
     6361 atgatgggag cgattatcaa tattggtgca gtaatcggtg aaaacacgat gattgatatg
     6421 ggtgctgtct taggtggacg agcgacagtg ggcaaaaact gccatattgg tgcaggagct
     6481 gtcttagcag gtgtgatcga accagcatcg gccaaaccgg ttatcgtaga agacggcgta
     6541 ctggtaggtg cgaatgcagt gattgttgaa ggtgtccata tcggaaaaga tgcagtcgtg
     6601 gcagcaggag cagtggtttt agaagatgtg gcagcagaaa ctgttgtcgg tggaatacct
     6661 gcacgtgtat taaaaataag tgatgatcag acaaaagaaa atacagcatt gattgctgcg
     6721 ttacgcgaat tatagaggag atggagaaaa atgcgcgaaa tagatacctg tattgtgggg
     6781 gcaaccggat tagtcggcca aacgatgctc aaagtgttag aagaatatga ttttccagta
     6841 ggacgattga aattattagc ttcaaaacgt tcagccggta aagtttgtgt attcaagaaa
     6901 aagaaacaca ctgtacagga attgacagaa acttcatttg aaggatatga tttagcgcta
     6961 ttttctgcag gtgcttctgt atccaaagag tttgcgccaa tcgcacgaag tcagggacta
     7021 tttgtcatag ataattcctc agcatggcgg gaagattcgg atattccatt aatcgttcca
     7081 gaggtcaatc tggaagacta tacattgaat cggctgattg caaatccaaa ttgttcaacc
     7141 attcaagcgg tacttccgct taaagcttta caagaagcct ttggtatcca gcgagtcaat
     7201 tattcgacct atcaagcagt ttctggttcc gggcaaaaag gcattgaaga tcttcatcgt
     7261 acgcaaaatg acgaggaacc agttttgtat ccttataata ttagccaaac ggtgattccg
     7321 gaaatcgatc agcctttgga aaatgggtat acaaaagaag aacagaaaat gataaatgag
     7381 acccgtaaga ttctgcatca gcctgctttg ccagtatcag caacctgcgt acgtgtgcca
     7441 atcgaaaatg gacatggagt ctctgtggca gttcaactgc aaaaagagtt tacgacggaa
     7501 gaaattagag agtgtctcag ccagtttcca gggattcaat tagaagatga tctagcagca
     7561 cataaatatc cgacgagcat tcttgctaga ggaacagata tggtttatgt tgggaggatc
     7621 agaaaagaca cttctttaga gaacggattg ttgttctata cgactgccga taacatacgc
     7681 aaaggagccg ctgcgaatgc cgtacagatt gctgcagctt taatgcagaa aatagcggag
     7741 gtggcaagat gagtaatacg ctgaaagtag cgaaattcgg cggaacatcg atgtctcatg
     7801 aagagcaatt caaaaaagtc cgtacaattg ttctttcaga tcccaatcgc caagtgatcg
     7861 ttgtttctgc tttaggcaaa cgtacgtcag atgatgacaa agtaacagac ttactttatt
     7921 tgatttatgc ttaccttcag catcatgttg attggacacc cctttggaaa aggatttgtg
     7981 atcgtttcat tcaagtaaga gatgttcttg ccctgcagtt acctattgaa gatgaactag
     8041 cacgcataca gaaacaactg gaagacgaaa agataactga agattattta gtaagtagag
     8101 gtgaatatct gactgctcaa ctgatggctg agtatcttgg ttaccagttt attgacgcaa
     8161 aagacctgat tttctttgac tatgatggga aaatcgatca ggaaaaaaca gcacaggaaa
     8221 tcacaaaagc atttaaaaga tatccgaaaa tggttgttcc agggttttac ggggcgaacc
     8281 ctgcaggaaa agaaaaatta ttgggccgtg ggggaagcga cattaccggc gcgatactgg
     8341 cgaagatgtt gcaagcggaa agttatgaga attggacgga tgtttcagga atcatgatgg
     8401 ctgatccgcg aatcatcgat catcctaaaa tcatcgagga aatctctttt agagaactga
     8461 gagagatggc ttatatgggg gctaacgttc ttcatgaagc agctgttttc ccagttcaag
     8521 aggctgatat ccccatccaa ataaaaaata ccaatgatcc tttcgctcaa gggacaaaaa
     8581 tatcgaatca cgaaatagaa aaggaaaatg gactgacagg aatagctgga cgcaaggatt
     8641 ttctttccat cactttattc aaacgtcata tgtccgatga aatcgggttt atctggaaag
     8701 caatgagcat ttttgctaaa catgagatat caatcgaaca tattccttca gggatcgaca
     8761 atatcggagt gatcgtctca gcagaagcga ttgccgataa gttatttctc atcacgaaag
     8821 aattaaaaga aacacttggc gtggaagaaa tcgtcgtgat cgaagactta gctttgatct
     8881 ctgtcgttgg gggtccgcat aaagaattga ttggattatc tgggaaagtg ttatcgatat
     8941 tgaacaaatt agaaattcgc acatcgatct tatcacaagg agcacaggag ttgaatctga
     9001 tcatcggtgt accgaataat caatatgaaa cagtagtgaa gggtatctat gaagggatgg
     9061 tgaatacgga tgctgaaaga atatcgaatg gcactccatc aaattcctga aatcgggttt
     9121 catgaattca agacaaaaaa atatttatat gaacaagtaa aaaacagcgg ggccgttatc
     9181 catgaaatcg gcgaaacagg actactcctt tattttgata atcagcaaga agaaacgatt
     9241 gcttttcgaa cagatatcga tgcactgcca gtcaaagaag aaacgaatct ctcttttgct
     9301 tctaagcatg aaggttatat gcatgcatgt ggtcatgacg gacatatggc gatgctttta
     9361 gggttgactg actttataac taaacacatt gaggatctta tttataatgt agtactgatc
     9421 tatcagccat cagaagaaat ttccggtggc gcacaaagcg ttattcagtc aggattgttg
     9481 gataaatatg aagtacaagc aatatttggc tttcatctat ggcctggact agctaaggga
     9541 gaagttttct ctcgaccagg acctttgatg gcacaaagca gtgaaacaga cattatcatt
     9601 aaaggccagt ctgcacatat tgcttctagt gaaaaaggaa tcgatagtct ggaggcagct
     9661 gtacgatttt tgaagaatgt ttatgatttt gaccggcaat taccggcaga gtggaatcat
     9721 ctaatgaaat ttggccaaat ccaaggtgga acgatccgca atgttttagc tggagaagtt
     9781 attgtatccg gaagtatccg ttcttacagt tcagatatgc aaaattattt gaagagggga
     9841 ttagaccatt tagcagaaca gtttcactct acagctacta tttcttttcg ctataatgac
     9901 ggctatccag ctgtaatcaa ccatagaggc ttatatgcag cactgttaga ttcaaacaaa
     9961 ctacatgaat tagatgctcc agttcttcaa gccgaagatt ttggcatata tacgcaacaa
    10021 tatccatgtg tatttttctt tttaggtgtc ggtgataccc ctgctttaca tgatccgacg
    10081 ttcgattttg atatggatgt tttagaaaat ggtgtagaat ggtacaaaac gattcttttt
    10141 acagaaaatc ttcttgtaaa aatcgatagc agttcaaaag aggagcgagt aaataatgat
    10201 gaatttatcc aatcgattta gtcctaggct ttctagaata gaagtatcaa aaatacgaca
    10261 attcgatcag caaatatcgt cgataccaga tgtgatcaaa ctcaccctgg gagaaccgga
    10321 ttttccaact cctgaacatg tcaagcaagc aggaatagca gcgattgaag aagacttttc
    10381 tcactatacc ggaatgagag gattagagga actgcgggaa gcggcttgta tatttcagca
    10441 acagcgttat ggattaactt atgatccgca aacggaagtt ttgacgacag ttggtgcaac
    10501 ggaagcgatt gcctctgcgt tgttatctgt tttggaagaa ggagacaagg ttcttattcc
    10561 agcgcctgct tattcaggat atcagccatt agtggaacta gctggtgctg aattgatccc
    10621 gatagatact tctgacacag ggtttgtctg tcagccggaa caatttgaac gagcatttga
    10681 acaatatgga tcagctgtca aagcagtcat tttaaactat cctaacaatc ctactgggac
    10741 gacattgtct gcaaaacaat tgaaagcaat cgctgaggtt ttaaaaaaat atccggtatt
    10801 tgttataagt gatgaagtat atgctgaatt gacttatagt ggaacgcata tgtccatagc
    10861 tacttatctt ccagaacaaa caatcgtgat aagcgggtta tccaagtcac atgcgatgac
    10921 tggttggcga gtgggcttta tttttgctca aaaagccctt attgacgaac taattaaagt
    10981 ccatcaatat ctggtgactg ccgctacaac gatgtcgcaa aaagctgcgg ctgaagcgct
    11041 aataaatggg gtgaaagata gcgagaatat gaaagagcaa tatcaaacac gaagagacta
    11101 tctggtccag caattggcgc ctttaggatt tgaagtgacc cagccaaacg gtgcatttta
    11161 cttgttttgc aaactcccag ctgccattca aactgactct tggcagtttt gtgtagaatt
    11221 agcggaacag gcaaaagttg cgtgtatccc aggaattgca tttggcccag aaggagaagg
    11281 ttatatccgg atttcttatg ctagcagcat ggaaaaatta catgaggcct gcaaacggat
    11341 caaagacttt ttaacagaac agtgattcat agattttatc aaaaaaacaa tcgttaattt
    11401 tgcggtatat tctgcaagga gacgattgtt ttttaaattt agttcaaatg aacgcgtttc
    11461 ttttgattca taactaatgt aatgttaagt ttaaattgtt tagaatgatt ttaaaagaat
    11521 attcaaggag gtttcatcaa tggaaaaaat caaagttgtc attgtaggtg catctcatgg
    11581 agggcatcaa tcgatcttgg aactattgac acgatacggt gaaaatgtag atatcacgct
    11641 atttgaagca ggagactata tttccttcat gtcatgtggt atggagctgt atttagaaga
    11701 caaggtaaca gatgtaaatg atgtacgtaa tttcagacca gaaaactttc cgcaatccaa
    11761 tgttcatatt ttaaataatc acgaagtaaa aacgatcgat gccgataaaa aaacagtaac
    11821 ggttactagc gcatcagata acgaaacaaa agaatatcct tatgacaaat tgattttgag
    11881 ttctggtgtc aaaccaaaca atctgccagt accaggagca gatctagaaa atgtctactt
    11941 gatgcgtgga tacaattggg caaccaaaat caaagaaaaa ttaaatgatc cggctattaa
    12001 aaaaatcgct gtcgtaggtg cgggttacat tgggatcgaa gcagcagaag caagccgaaa
    12061 agctgggaaa gaagtcgtat tgcttgatgt gatcgataga cctttaggca cttatttaga
    12121 tgctgagatg acagatattt tagaacaaca cctaaatgaa aacggtattg aagttttgac
    12181 aaatgcaaaa atcgaagctt ttactggtaa cggaaaagtt gaggcaatca aaacaagtga
    12241 aaaagaaatc ccagcagatc tagtcattca agctgccggt gtgaaggcaa atacagaatg
    12301 gttaaaaggg atcgtcgact tagatgaacg tggctggatc caaacaaatg aatacttgca
    12361 aacgaatctt ccagatgtgt atgcagtagg agatgctaca cttgcttatt ctatcccagc
    12421 acgcaaaaaa atgccgatcg ctttagcgac tgttgcacgt agagaagcac gttatgtagt
    12481 aaaacatctt tttgaagaag taccaaacaa accgttcggc ggagtcgtag gttcctctgc
    12541 attaagtgtc tttgactatc attttgcagc aagcggattg aatagcttta cagcagcaaa
    12601 agcaggagta gatgtacgag tgtcttatta tgaagatacg ttacgaccaa aatatgtacc
    12661 agaagaacaa ggaaatccaa aagtgtccat ccaattattc tcggatccaa tgacccatca
    12721 attgctagga ggagcagtat tatctaccta tgatgttaca ggtcaaggaa atgtattggc
    12781 actagcgatc caaaacaaac tgactttaga agaactagca gaagctgact tcttcttcca
    12841 gccaggcttt gaccgacaat ggagcatgtt gaatctagcc gctcaacaag cgttaggaga
    12901 agaaccattt gtagaataag aaaacgaaat atttgaaaag aggttgtgaa aaagctgcct
    12961 tgcatcaagt aataagaaca gccaagtaaa aacagcttct tatgttttta acttggctgg
    13021 acttattacc gcagatgcaa gcttttgaac accgtttatt ttcaaaaaag ggactgtgac
    13081 acgatttgtg taccgacccc caaaagttag accaaaaaat ctaacttttg gaggtcggta
    13141 ttttttatgg ttaaatatac ttttgaactt aaattaaaga tcgttcatga ttatttggat
    13201 ggcaaaggcg attggaccgt aaaaatccag atcaagagat tgaagcggaa atgcttaaaa
    13261 ttcgggagaa gcacaaggat tatggctgtt tacggatgac caatgaacta agaaaccgcg
    13321 gtttctctat caataaaaag aaagttcagc gcttgatcaa gaagctagga ataaaagtaa
    13381 cggcatacac gcgtaaatca cgtcgctaca attcttatcg aggaaaagt
//
LOCUS       DACEEZ010000078        13223 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20313_2017
            SAMN12639966-rid9879223.denovo.078, whole genome shotgun sequence.
ACCESSION   DACEEZ010000078 DACEEZ010000000
VERSION     DACEEZ010000078.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639966
            Sequence Read Archive: SRR10023158
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13223)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13223)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741683.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741683.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:44:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,016
            CDSs (total)                      :: 2,962
            Genes (coding)                    :: 2,889
            CDSs (with protein)               :: 2,889
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 36 of 73
            Pseudo Genes (incomplete)         :: 29 of 73
            Pseudo Genes (internal stop)      :: 18 of 73
            Pseudo Genes (multiple problems)  :: 8 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13223
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639966-rid9879223.denovo.078"
                     /isolate="E20313_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            158..1027
                     /locus_tag="IWO89_002237"
     CDS             158..1027
                     /locus_tag="IWO89_002237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extradiol dioxygenase"
                     /protein_id="HAQ0456955.1"
                     /translation="MKDFQLGAATYLKSVAIRVKDRDKMIDFYKNVVGFDLKREENEL
                     AILGTEEAGSEMLLLEESPRANDHMGVIKKLNRFSLIIPSTEEMADILCRIRKMNYPI
                     KEALEDHGRMGILLVDPEENELEIYHEENKNDQREIPETMDQEALLRKSEEKFSKLST
                     GSYFEKIHLNVTNLTKERQFLNHVLGLKVHNEEEGIHVLNEGDFHVGLTEASGGAIDL
                     PTDEVIGLDFLKFMIDTESMEGLIAHLQQLSVDFFLDKKHTVLTIYDAIGIEWWFVSE
                     PKEKNKTDIRCDA"
     gene            1236..2261
                     /locus_tag="IWO89_002238"
     CDS             1236..2261
                     /locus_tag="IWO89_002238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-lactamase family protein"
                     /protein_id="HAQ0456956.1"
                     /translation="MYPHTKQLILKKMEEGFYPGVAVAFIKENQVETFTAGFAQVVPE
                     KQLMTEDLLFDAASLTKVVATTSLVLRLLEDGKIELDQPLHKYLPEFENQTITLRHLL
                     THTSDIKTWIPNRDKLSQEELIAAYLKLEPGEQLGKEVKYTDAGTILLGLMLEEIYQE
                     TAVEIFQEQVLEPLGMMNSLFLPFPSKKIVPTEQLPSGEVLRGQTHDPKARVLAEHAG
                     NAGLFIDLKDSITFVQMYLNKGKIRNGRFLEEETIDSLLQDQTPKKNGYRSLGWDLKK
                     DVEDGHAILFHTGYTGTFFLIDIKTQEALIFLSNRVHPVDHRAEYLICRDEIVENFLK
                     EKAVLPK"
     gene            2300..3250
                     /gene="manA"
                     /locus_tag="IWO89_002239"
     CDS             2300..3250
                     /gene="manA"
                     /locus_tag="IWO89_002239"
                     /EC_number="5.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-6-phosphate isomerase, class I"
                     /protein_id="HAQ0456957.1"
                     /translation="MQEPMFLKPVFQEKIWGGSRLRSVFGFDIPNDKIGEDWAISAHP
                     HGVSVVENGEFKGKRLDELWSEHKELFGHPSEPVFPLLIKILDAEDDLSVQVHPDDAY
                     GMKHEGELGKTECWYIIDAEPGAEIIYGHHAKTREELAEMIKDGRWDDLLKKVPVKKG
                     DFFYVPSGTIHAIGKGIMILETQQSSDTTYRVYDYDRKDAQGQTRELHIQQSIDVTTV
                     PAKTPELQIKEVRKGNSSIVTYLETEFFNVYEWDIKGITSFKKQAPYTLMTVIEGAGD
                     LVVDGKTYPLEKGTSCIIPNGVSEWAVQGELAIIATEPGK"
     gene            3344..5047
                     /locus_tag="IWO89_002240"
     CDS             3344..5047
                     /locus_tag="IWO89_002240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAQ0456958.1"
                     /translation="MEYKWKANNSSRVSMKQLRKAGHHLNEMIMPNLSLFIAWGILSL
                     ATKGVSGDLHTTLEEVCHWMIQLLLPILISYSGGKSVGGQKGAVAGAIASLGLIARSN
                     APQIVGAMIIGPLAGIVYEQFVSRFHVKFKAGYEMLVSNLLVGLTGSVICIIGIVFIE
                     PVLNSVHLLLASVVSWLVTMNLLPLVSLILEPLKVLFFNNAINHGVLTPLGIEFSQTV
                     GYSSLFLMEANPGPGLGILLSILCFAEKQEKVNASGALMIHAIGGIHEIYFPFVLLRP
                     YLFLAVMAGGASGTLIFQWLDTGLAAPVSPGSLLVIFANAPYPQLAGVSIGIAISTFV
                     TFICAAILLKLPNKEKENKTVKERGEILEPSIIHSVIFACDSGMGSSAMGAALLKKKL
                     AAEKIDLSVGYSSIYELNNQVNQLVIVQHELAGLAREKVPQSQIYVLDHFLETDILLS
                     RIKEAIQFPKREEKKEIADITEQKKSPANIVFLYKNNRRGSQTMGMTILSQLAKKAKK
                     QFVISKESIEKIKIQEETIYIAQRELAVTEQLEEKIPRLVLVEHWVATNEYEKLVKGE
                     I"
     gene            5049..7082
                     /locus_tag="IWO89_002241"
     CDS             5049..7082
                     /locus_tag="IWO89_002241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator"
                     /protein_id="HAQ0456959.1"
                     /translation="MYLSPREKQLLAEFLSSPSPVSIQKMMNLLKVSKRTIYRELDNL
                     EVSLKSVNASLKKAARGSFVIEASEEVLEQLKENTDGKSNDLSTAKRQHAILAELLLT
                     TEPLSLAHFMESYRISNTTFYADIKQLEESIRQLPLEIIRNQGYEIEGPEKYRRLLTA
                     NVLELEINEYELFHSISFDASLNYFFRFVDPQHLSLARKVIGEELIQKKTNLSDRKLE
                     HLVLMLTLTMERVTKNHLLTNETYNSLANKELLQTAKRLFSRIASETKQLYPVNEIVF
                     FAGLLNDFTNSFDQNFFEETFDTQLAYLVKQLIQEVSEETNVHFYEDETLYKMLLTHL
                     SGVFSRAVLQEETLTNPILEKIMVQYEEVANAIRKAVERIFQEKKLSEEEIAYMVLHF
                     ANSLERSPKESNISIAGFSPSGLASTSMLELRLKRYFPFIQQIHFYRIADLKKIDLQE
                     EYDVVISTSILPGYGGKYILVSPLLLEDEVKQLKEAFSHIEKKKRQKITYNSNNEWMI
                     EDTYEETMHFMDQINQLLGRFFIQELDNPETISETIRSLFPYFPEGLVTDEEVVHRRL
                     LKRYEQAPIGIPHTNMGLFHTSSSLISKPIFCIFNLKHPLTIEGMDRQPVDLSRMLVM
                     LAPSPIQEKDGILLGKISGAIIMNDLNTEIFRSGNKEIVYQLLAKILIEEIKK"
     gene            7093..7533
                     /locus_tag="IWO89_002242"
     CDS             7093..7533
                     /locus_tag="IWO89_002242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HAQ0456960.1"
                     /translation="MIQLPAEKILLDQSYQNWKEALTEIGQWMTQQGGIKPAYIQSMI
                     ERQEKASVYIGNFVALPHGTDKDEEVVEEGIYLVQVPDGVDFGTKEEPKIATLLFVVA
                     LKKEHQLAVLQDLAFFCSDIDQVMALSDAKTIEEVQMIFEHGHI"
     gene            complement(7733..8860)
                     /locus_tag="IWO89_002243"
     CDS             complement(7733..8860)
                     /locus_tag="IWO89_002243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAQ0456961.1"
                     /translation="MSLLTFERNLLLKNKVNFLFPLLLVVLFAFPLFFDHKLAYTEFD
                     ELNHNYEEMQRLIETLKEDENEKEFVESLEKSNKLIEAILHAKNTGNVQQMVEATYHY
                     EKDILDRLISGQRQGIPIIEQQKRVELLRYMKEHQIQRYSIFDLPAHLPLANYYENIF
                     SGMISSFLILCITALFLSSIISYEKRKQVISLVNLLPDSMVKKHSIRFTIYYGAAMLS
                     LVMPFLIVSILVIIKNGLGDFRYPVGTIIGQEIRILPMYEYLFQSFLFLLLWVLFLST
                     ISFLLSALFEHSLVNLLGTLLCLFLAEYRLFSSIGWIESISHYLPTSYVDFQNVIIGG
                     DIFSPLASEQVTFMNGILTLGIWSIVLLFIGMGTIYIKKSY"
     gene            complement(8863..9540)
                     /locus_tag="IWO89_002244"
     CDS             complement(8863..9540)
                     /locus_tag="IWO89_002244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ0456962.1"
                     /translation="MTLLIKNMNMKRGKKRVIRQLNLLVESGEVAALIAPNGFGKTTL
                     LNGIAQLLPTTYDQLSLHNISPTAYTDYRRSFFFLESTNQLSDYLSAVDYLTLIKYYW
                     QSKVECQTVFQRLKMTEYLTLPIGKMSLGMKQHVLLAAYLISDSSVMLFDEPLNGLDP
                     GSIDILNSLIRQWQKEGKTILLSSHAIANIQSICSKACFLKNGEIVTESSNMEEILAI
                     YRKLYEV"
     gene            9804..11570
                     /locus_tag="IWO89_002245"
     CDS             9804..11570
                     /locus_tag="IWO89_002245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannitol transporter subunit IICBA"
                     /protein_id="HAQ0456963.1"
                     /translation="MERTIPKNNISLKARVQKLGSTLSSMVMPNIGALIAWGVLTALF
                     IPDGYLPNEALATMVSPMLTYLIPLLIGYTGGKVIAGDRGSVVGAIATMGVIVGTDIP
                     MMLGAMIMGPLGGYAIKKFDQLFQKRIKSGFEMLVNNFSAGLIGFGLALLGFSAIGPV
                     VDALTQAMAKGVEIILSAHLIPLTSIFIEPAKILFLNNAINHGILTPLGTEQVAEAGK
                     SILFLLEANPGPGLGVLLAFTLFGKGAAKSSAPGAMIIHFLGGIHEIYFPYVMMKPLL
                     FLSVIGGGMSGSFIFQLMDAGLRAPASPGSIIAILAMTPFNSYLPVILGVIVAAAVSF
                     GISAVILKADAKDTNEDFEKSVQETKAAKQASKGAQGIPQGATFSGVQKIIFACDAGM
                     GSSAMGASILRKKVQEADLPQTVTNQAISQLTDDSNTLIVTQVELQERAKQKAPSARF
                     VSVENFLNSPRYDEIIQELSSKQKTSVSEIENEPAEEFLSANIKQIKEILLVHDDRSG
                     SATMGMTVLKDILDKNHLTIPLRKVNIQDLKDEPDSLIVAKESLAEKVQMAAPQSIHL
                     SVNSMVNPQKYESIAQYLKQTA"
     gene            11585..12022
                     /locus_tag="IWO89_002246"
     CDS             11585..12022
                     /locus_tag="IWO89_002246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HAQ0456964.1"
                     /translation="MQSLPFDMIELNRSFKTKEEAIRYCGQMLVEAGCVDEEYIDAMV
                     DRDKMLSVYMGNFIAIPHGTDEAKKYVKKSGICVVQVPDGVDFGTEEEEKIATVLFGI
                     AGVGDEHLQLVQQIALYCSDMDNVVQLADALSKEEVTENLAIA"
     gene            12035..13186
                     /locus_tag="IWO89_002247"
     CDS             12035..13186
                     /locus_tag="IWO89_002247"
                     /EC_number="1.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol-1-phosphate 5-dehydrogenase"
                     /protein_id="HAQ0456965.1"
                     /translation="MRAVHFGAGNIGRGFIGEVLAANGFEITFVDVNHTIIDALNERK
                     EYEIELADESKEKITVKNVKGINNQTNPEKVIQEIAEADLVTTAIGPNILPFIAELIA
                     KGIQEREKEGSTTSLDIIACENMIGGSAFLEKEVYKYLPETSFTDKYIGFPNAAVDRI
                     VPLQHHEDPLFVQVEPFKEWVIDDSQRKNKKIQLKGVLYVDDLEPYIERKLFSVNTGH
                     ATVAYTGALLGYQTIDEAMQDALVVAQLKSVLQETGSLLIAKWGFDAEQHHAYIEKII
                     HRFQNKNISDAITRVARTPLRKLGYQERFTRPVRELQEHNLTCPHLTATMGIIFNYYD
                     PEDEQSRQLHEMKIHENLEQLIQEVTGINDPKTIGNIKQNVNRYAKQVA"
BASE COUNT         4491 a         2298 c         2731 g         3703 t
ORIGIN      
        1 cacaacctct ttttataaga gtttttactc attaatttac aaaaaacaag caaaatgcga
       61 attttctcac atttttttta acaaaatcaa agttattgct tgaaagaaga agaatccatc
      121 gttaaaataa agcttgacgt agaggggagc aagaaatatg aaagattttc aattgggggc
      181 tgccacctat ttaaaaagtg tagccattcg ggtgaaagac cgagataaaa tgattgattt
      241 ttacaagaac gttgttggct ttgatttgaa aagagaagaa aacgaattag ccattttagg
      301 aacagaagaa gctggaagtg aaatgttgct gctggaagaa agtcctagag cgaatgatca
      361 catgggggtg atcaaaaagc tgaaccgttt ttcattgatt atccctagca cagaagaaat
      421 ggcagatatc ctttgtcgta tccgtaaaat gaattatcca atcaaagaag ctttagaaga
      481 tcatggacgt atggggattt tgctcgtaga cccagaagag aatgaattgg aaatttacca
      541 tgaagagaat aagaacgacc aaagagaaat tccagaaaca atggatcaag aagcattatt
      601 aagaaaatct gaagaaaaat tcagtaaact atcaacaggt tcttattttg aaaaaatcca
      661 tttaaatgtt actaacttaa caaaagaacg ccaatttctt aatcatgtac ttggtttgaa
      721 ggtacataat gaagaagagg gcatccatgt attgaatgaa ggagatttcc atgttggttt
      781 gacagaagct agcggaggcg caattgattt gcctacagat gaagtgattg gacttgattt
      841 tctgaaattc atgatcgata ctgaaagcat ggaaggactg attgctcact tgcagcaact
      901 atcagttgat ttcttcttag acaaaaagca cacagtactc acaatctatg atgctatagg
      961 aatcgaatgg tggttcgtca gtgagccaaa agaaaagaat aaaactgaca ttcgatgcga
     1021 cgcttaactt tttctttctt aacgattgtc gcatgtctcg tcattttatg aacggtcata
     1081 ctatcgctta ttctagagat agaaagagaa aatagtatac cgggggaaac tgcgcggttt
     1141 ttcgcgcagt ttttttgtgt aaaaaaggtt gattcctcat agattaagct ttaagcttgg
     1201 actgtaagaa tctgatagaa aagcaggaga aaaagatgta tccacacacg aaacagctta
     1261 ttttgaaaaa gatggaagaa ggattttatc caggtgtagc agtcgccttc ataaaagaaa
     1321 atcaagtaga aacatttaca gccggctttg cccaagtcgt tcctgaaaag cagctgatga
     1381 cagaagatct gttgtttgat gcagcctcac tgacgaaagt cgttgcaacc acttcattag
     1441 ttttacgtct attagaagat ggaaagatag aactcgatca accgcttcat aaatatcttc
     1501 ctgaatttga aaatcagaca atcactttgc gtcatttact gacacatact tctgatatca
     1561 aaacatggat accaaacaga gataagctat ctcaggaaga attgatcgcc gcttatttga
     1621 agctagaacc aggtgagcaa ctaggaaaag aagtaaaata tacagatgcc ggtacgattc
     1681 ttcttgggtt gatgctagag gaaatttacc aagaaactgc agtggaaatc tttcaagaac
     1741 aagtattgga accattagga atgatgaaca gcttatttct tccttttcct tccaaaaaaa
     1801 tcgttcccac agaacaactg ccttcgggtg aggttttgcg tggacaaact catgatccga
     1861 aagcaagagt tttggctgaa catgcaggta acgcgggtct ctttattgat ttgaaagaca
     1921 gcattacttt tgtccaaatg tatttgaaca agggaaaaat ccgtaatggt cgttttttag
     1981 aagaagagac gattgattct ttactgcaag atcaaacacc gaaaaaaaat ggctatcgtt
     2041 ctttaggatg ggacttaaaa aaggatgtag aagacggtca tgcgattctt tttcatactg
     2101 gttatacagg tacgtttttc ttgattgata taaaaacaca agaagcgttg atatttttgt
     2161 caaatcgcgt acatcctgtc gatcatcgtg cggagtatct catatgtcga gatgaaattg
     2221 ttgaaaactt tttgaaagag aaagcggtat taccgaaata atgctataat agctatgaaa
     2281 aatgagagga gtttgaatta tgcaggaacc tatgttttta aaaccagttt ttcaagaaaa
     2341 aatttgggga ggaagtcgtt tacgttcagt ctttggtttt gatatcccta acgataaaat
     2401 cggagaagat tgggcgatca gtgcgcatcc tcatggtgtc agtgtagtag aaaatggcga
     2461 attcaaaggg aaaagattag atgaactatg gtcagaacac aaggaattgt ttggccatcc
     2521 ttcagaacca gtgtttccat tattgatcaa gatcttagac gcagaagatg atctatctgt
     2581 acaagtacat ccagatgatg cctatgggat gaaacatgaa ggtgaattag gaaaaactga
     2641 atgctggtat atcatcgatg ctgaacctgg tgcagaaatc atctatggtc atcatgcaaa
     2701 gacaagagaa gaactagctg agatgatcaa agatgggcgc tgggatgacc ttttgaagaa
     2761 agtaccagtt aaaaaaggtg atttctttta tgtaccaagc ggaacgatcc atgctattgg
     2821 taaaggaatc atgattttag aaacacagca aagttctgac acgacttacc gcgtgtatga
     2881 ctacgacaga aaagatgcgc aaggacaaac aagagagctg catatccaac aatcgatcga
     2941 tgtaacaact gtccctgcga aaacaccaga attacaaatc aaagaagtcc gaaaagggaa
     3001 ttcatcgatc gtcacttatt tagaaactga gtttttcaat gtatatgagt gggatattaa
     3061 aggaatcaca tctttcaaaa aacaagcccc ttatacattg atgacggtaa tcgaaggagc
     3121 aggagacctt gtcgttgacg gaaaaacata tccattagaa aaaggaacaa gctgtattat
     3181 tccaaatggc gtttctgagt gggcggtcca aggtgaacta gcaatcattg cgactgaacc
     3241 aggcaaatag ttcccagata gctgaaagca gcagacggtt tttcgtttgc tgctttttct
     3301 acataaaatc cgtccatcaa taaatagaag tatggtgaaa aagatggaat acaaatggaa
     3361 agcaaacaac agcagtcgcg tgtctatgaa acaactgaga aaagctggac atcacttaaa
     3421 tgaaatgatc atgccaaatc taagtctgtt tatcgcttgg ggaatcttgt cattagcaac
     3481 aaaaggtgtg tctggcgatt tgcacaccac attagaagag gtatgccatt ggatgatcca
     3541 gctgctttta cccatcttga tcagttattc aggcgggaaa tcggtagggg gacaaaaagg
     3601 ggcagtcgcc ggtgcaattg cttctttagg gttgatcgct cgttccaatg ctccacaaat
     3661 cgtaggagcg atgattatcg ggccgcttgc cggcatcgta tatgaacaat ttgtttctag
     3721 attccacgtg aaattcaagg ctggatatga aatgcttgtc agcaacctac tagtaggact
     3781 gactggtagc gttatttgta taattggaat cgtttttatt gaaccagttt taaatagtgt
     3841 tcacttgttg ctcgcctccg ttgtgtcttg gctagtcaca atgaatctgt taccacttgt
     3901 cagtctgatc ttagaacctc taaaagtact atttttcaat aatgcaatca atcatggtgt
     3961 attgacacct ttaggaatcg aattctcaca aacagtcggg tactcttcct tatttttgat
     4021 ggaggcaaat cctggacctg gtttagggat cttactttct atcctttgtt ttgcagaaaa
     4081 acaggaaaaa gtaaatgcga gtggagcatt gatgatccat gcgattggcg ggattcacga
     4141 aatctatttt ccatttgtct tgctgcgacc gtatctattt ttagctgtaa tggctggtgg
     4201 ggcaagcggc acgttgatat ttcaatggtt agatacggga ttagctgcac ctgtatcgcc
     4261 tgggtcacta ctcgtgattt ttgccaatgc gccttatcca caactagctg gggtaagtat
     4321 agggatagca atcagtacat ttgtgacctt catttgtgca gcgatactat tgaaattacc
     4381 aaataaagaa aaagagaaca aaacggtcaa agaaagggga gaaatcttgg aaccatctat
     4441 cattcattcc gtcatttttg cttgcgactc aggtatggga tcaagtgcta tgggagccgc
     4501 cctgctgaaa aagaaattag cagcagaaaa aatcgatctt tctgtcggat acagctctat
     4561 ctatgaattg aataaccaag taaatcagct ggttatcgtg caacatgaac tagcgggtct
     4621 tgcaagagaa aaagtacctc aaagtcaaat ctatgtactg gatcactttt tggaaacaga
     4681 tatcttgctg tcacgaataa aagaagcgat ccaatttcca aagcgggaag aaaaaaaaga
     4741 gatagcagat atcacagaac aaaaaaagtc gcctgctaat attgtatttt tgtataaaaa
     4801 caacagacgc ggctcacaaa ctatgggcat gacgatttta agtcaattag caaaaaaagc
     4861 gaaaaaacag tttgttatct caaaagaatc aatagaaaaa atcaaaatcc aagaagaaac
     4921 gatttatatc gcacaaagag aactagctgt aactgaacag ctagaagaaa agatccctcg
     4981 gctagttcta gttgaacatt gggtagctac gaatgaatac gaaaaactcg tgaagggaga
     5041 aatataagat gtatttatcg cccagagaaa aacaattatt agcagaattt ctcagtagcc
     5101 cgtcacctgt atctatccaa aaaatgatga atttattgaa ggtcagtaaa cggactattt
     5161 accgcgaatt agataatctt gaagtctcat tgaaatcggt aaatgcttct ttgaaaaaag
     5221 cagctagagg ctcatttgtc attgaggcat cagaagaagt attagagcaa ttaaaagaaa
     5281 atacagatgg gaaaagtaat gacttatcca ctgctaaacg acaacacgcg attttagccg
     5341 agttgttact gacgacagag ccgttgagtc ttgctcattt catggaatca tatcgcatca
     5401 gtaataccac tttttatgca gatatcaaac agttggaaga aagtattcgc caactgcctt
     5461 tggaaatcat tcggaaccag ggatacgaga tcgaaggacc ggaaaaatac cgccggcttc
     5521 tgacagcgaa tgtattagaa ttagaaatca atgaatatga actttttcat agtatttctt
     5581 tcgatgcatc actgaattat ttttttcgat tcgttgatcc gcaacatcta tctcttgcac
     5641 gcaaagtaat cggggaagaa ttgatccaaa aaaagaccaa tctaagtgat agaaagcttg
     5701 aacatttggt tttgatgctg acattgacga tggagcgtgt gactaaaaat catttactga
     5761 caaatgagac atataatagt ctggcaaaca aagaattact acagacagct aaacgattgt
     5821 tttcacggat cgcttctgag acaaaacaac tttatccagt aaatgagatc gtcttttttg
     5881 caggattatt aaatgacttc acgaactcat ttgatcagaa tttctttgaa gagacgttcg
     5941 atacacagct tgcttattta gtaaaacaac tgattcaaga agtaagtgaa gagacgaatg
     6001 tccattttta tgaagatgaa actttgtaca aaatgctgtt gactcattta tctggtgtgt
     6061 tttcaagagc cgttttacaa gaagagacac tgaccaatcc gattttagaa aaaatcatgg
     6121 ttcaatacga ggaagttgcg aatgcaatcc gtaaagcagt agagcggatt tttcaagaaa
     6181 aaaaattatc cgaagaagag atcgcttata tggtccttca ttttgcgaat tcattggaac
     6241 gaagtccgaa agaaagcaat atcagtattg caggattttc gccaagtgga ttagcttcta
     6301 ctagcatgtt ggaactacga ttgaaaaggt attttccctt catccaacaa attcatttct
     6361 atcggattgc tgatttaaaa aaaatcgatt tacaagaaga atatgatgtt gttatttcta
     6421 catctatttt accaggatac ggtggaaaat atattttagt ctccccgcta ctgctagaag
     6481 atgaagtcaa gcagctaaaa gaagcttttt cgcatattga gaagaaaaaa aggcaaaaaa
     6541 ttacgtataa cagcaataac gagtggatga tagaagatac ttatgaggaa acaatgcatt
     6601 tcatggatca aatcaatcaa ttactcggtc gtttttttat ccaagaactg gataatcctg
     6661 agacgatttc cgaaacgatt cgcagcttat ttccttattt tcctgaagga ctagtaacgg
     6721 atgaagaggt ggttcatcgc cggctgctta agcgatatga acaagcacct atcggtattc
     6781 ctcatacaaa tatggggtta ttccatacat ccagctcatt gatttctaaa ccgatctttt
     6841 gtattttcaa tttgaaacat ccgttgacca ttgaagggat ggatagacag ccagtcgatt
     6901 tgtcccgtat gttagttatg ctggcgcctt cgccaatcca agaaaaagac gggattcttt
     6961 taggaaagat cagtggagca atcatcatga atgacttgaa caccgagatc ttccgctcag
     7021 gaaataaaga aatcgtttat cagttgttag cgaaaatcct gattgaagaa ataaagaaat
     7081 gaaggtgaaa agatgataca gctaccagca gaaaaaatat tgctcgatca atcctatcaa
     7141 aattggaaag aagcgttgac cgagatcggc cagtggatga cccaacaagg gggaatcaaa
     7201 ccggcatata tccaatcaat gatcgaaaga caagaaaaag cgagtgtcta tatcgggaat
     7261 ttcgtagcct tgccacatgg gactgacaaa gatgaagaag tagtagaaga aggaatctat
     7321 ttagtccaag tgcctgatgg tgtggacttt ggaacaaaag aggaaccaaa aatcgcgaca
     7381 ctcttatttg tagtggcttt aaaaaaagaa catcagttag cggtgctcca agatttagcc
     7441 tttttctgtt cagacatcga ccaagtaatg gcgctcagtg atgcgaaaac cattgaagaa
     7501 gtccaaatga tttttgaaca tggacatata taaccaatgt tcacagtaaa aaaagctgcc
     7561 ttgcatcaag taataacttc aaccaagata aaaataagag gagctatttt tgttgggagg
     7621 ttcttattac tgcggatgca agcttttgaa aactatttat tctagaaaac cgtttatttg
     7681 aaatagggga cggtgacaag gtttttgtca ccgcccctat tatttttgtg ttttaatagc
     7741 tcttttttat atagatagtg cccataccta tgaataataa aacgatactc catataccaa
     7801 gtgtcaagat accattcatg aaagtaacct gttctgaagc tagtggcgag aaaatatctc
     7861 ctcctatgat cacattttga aaatcaacat aagaagttgg caagtaatga ctgattgatt
     7921 ctatccatcc aatcgaagaa aacaagcgat actctgctaa gaaaagacaa agaagtgtac
     7981 ctaaaagatt gactagagaa tgttcaaata aagcagatag aagaaaactg attgtactta
     8041 aaaagagcac ccacaacaac aggaacagaa agctctggaa aagatactca tacatcggaa
     8101 ggattcgtat ctcctgacca atgatcgttc ctacaggata acggaagtct cccaaaccat
     8161 ttttgattat tactagtata gagacgatca aaaatggcat cacgagactt agcatagctg
     8221 caccataata aatggtaaaa cggatgctat gtttctttac catactatcc ggtaatagat
     8281 tgactagaga gattacctgc tttctttttt cataagagat gatggaagaa agaaacaagg
     8341 cggttataca taaaatcaaa aaactagaaa tcattcctga aaaaatattt tcgtagtaat
     8401 ttgctaaagg gagatgcgct ggcaagtcaa aaatagaata tctttggatc tgatgttctt
     8461 tcatataacg gagcaactcg acacgttttt gttgttcaat gattggaatt ccttgtcttt
     8521 gaccagaaat cagtcgatcc agtatatctt tttcataatg ataagttgct tcgaccattt
     8581 gctggacatt ccctgtgttt tttgcatgta agattgcttc gatcagtttg ttgcttttct
     8641 ctaaactttc cacaaattct ttctcattct catcttcttt caacgtttca atcaaacgtt
     8701 gcatctcttc atagttatga ttcagttcat cgaattctgt ataagcaagt ttatgatcaa
     8761 aaaaaagtgg gaatgcaaag agcacgacca acaatagagg aaataaaaag tttactttgt
     8821 tttttagtaa gagattgcgt tcaaatgtaa gtaaagacat agttacacct catataattt
     8881 acgataaata gctaaaatct cttccatatt tgaggattct gtgacgattt ctccattttt
     8941 tagaaaacat gctttagaac aaatcgattg gatattcgcg atagcgtggg aagaaagaag
     9001 aatcgtcttc ccctctttct gccattgtct gatgaggcta ttcaagatat caatacttcc
     9061 tgggtctaat ccgttcaacg gttcatcgaa taacatgaca gatgaatcac tgatcaagta
     9121 agcagcaagc aagacatgtt gtttcattcc tagagacatt tttccaatcg gcaaagtaag
     9181 gtactcagtc atcttcaatc gttggaaaac agtttggcat tctactttgc tctgccaata
     9241 gtatttgatc aaagtcaaat aatcaacagc cgatagatag tcggataact gattcgttga
     9301 ttctagaaaa aagaaagacc ggcgataatc tgtataagca gtaggagaga tgttgtgaag
     9361 ggacaattga tcataagttg ttggcagcag ttgggcaatg ccattcagca aagttgtttt
     9421 gccaaatccg ttcggggcaa tcaaagcagc tacttctcct gattctacta aaagattcaa
     9481 ttgtcgaatc acacgttttt taccacgttt catgttcatg tttttgatca gtaatgtcat
     9541 agataggcct ccaatataaa atttaataac ataatagaat ggaaaacatc taaattaaat
     9601 gctaacaaaa gttattgttt gcatattata aaaaaattaa aataacagtt ttatttaaaa
     9661 ttgaagtttt ctgataccga aatcttttta ttttgctgac atactcaaac ctttggcaca
     9721 aagtttcgtg ccaaagtacg actatttctc tttttagtaa gcggatacaa ttagagtatc
     9781 aaaaggagag agggagagac aaaatggaac ggacgattcc caaaaacaac atttcgctaa
     9841 aagcgagggt acaaaaatta ggcagtactt tatcaagcat ggtcatgcca aatattggag
     9901 cactgattgc ctggggtgta ttaacggctc tatttatccc agatggttat ttaccaaatg
     9961 aggctttggc taccatggta agcccaatgc taacttatct gattccttta ttgatcggtt
    10021 atactggcgg aaaagtaatt gctggtgacc gcggatcagt tgttggggca attgcaacga
    10081 tgggtgtgat tgtcggaaca gatattccaa tgatgctagg cgcgatgatc atgggacctc
    10141 ttggtggata tgccatcaag aaatttgatc agctattcca aaaacggatc aaatccggtt
    10201 ttgaaatgct agtcaataat ttttcagcag gtcttatcgg ttttggatta gcgttattag
    10261 gtttttctgc gattggtcca gttgtcgatg cattgactca agcaatggca aaaggtgtag
    10321 agatcatttt atctgcccat ttgattccat tgacaagtat ttttattgaa ccagcaaaaa
    10381 tcttattttt gaataatgca atcaatcatg ggattttgac accattggga actgaacaag
    10441 tagcagaggc tggaaaatct attctattct tgcttgaagc aaatcctggt ccaggacttg
    10501 gcgtattact tgcttttact ttgtttggca aaggagcagc aaaatcttca gcaccaggag
    10561 ccatgatcat tcacttttta ggcgggatcc atgaaatcta ttttccttac gttatgatga
    10621 aaccgttatt attcttatca gttataggcg gaggaatgtc aggaagtttc attttccaat
    10681 tgatggatgc tggactaaga gcgccagctt ctccagggtc gatcattgcg attctagcga
    10741 tgacaccgtt caattcttac cttcctgtta ttttgggcgt gattgttgcc gcagctgttt
    10801 cgtttggtat ttctgcagta attttgaaag cggatgcgaa agatacaaat gaagacttcg
    10861 aaaaatctgt tcaagagaca aaggcagcta agcaagcttc caaaggtgcc caaggtattc
    10921 ctcaaggagc tacattctct ggcgtacaaa aaatcatttt tgcttgtgat gcaggaatgg
    10981 gatcaagtgc gatgggcgct tcgatcttga gaaaaaaagt acaggaagca gacttgcctc
    11041 aaacggtgac caatcaagca atcagccaac tgacagatga ctcgaataca ttgatcgtta
    11101 ctcaggtcga actacaagaa agagcaaaac aaaaagcacc gagcgcgcgt tttgtctcag
    11161 tagaaaactt tttgaattca cctcgatatg atgaaatcat tcaagaatta agcagcaagc
    11221 agaaaacatc agtttctgaa attgagaatg agccagcaga agagtttctt tcagcaaaca
    11281 tcaaacaaat caaagagatt ctacttgtcc acgatgatcg ttccggatca gcaacaatgg
    11341 ggatgaccgt gctcaaagat atattagaca agaaccattt aactatcccg ttaagaaaag
    11401 tcaatatcca agatttaaaa gatgaacctg attcactgat cgtagccaaa gaaagtttag
    11461 cggaaaaagt acagatggct gcacctcaat ccattcattt atcagtcaac agcatggtca
    11521 atccgcaaaa atacgaatcg attgcacaat atttgaaaca gacagcttaa gggaggaaaa
    11581 cagcatgcaa agtttgccat tcgacatgat cgagctcaat cgatctttta aaaccaaaga
    11641 agaagcgatc cgctattgcg gtcagatgct tgtagaagca gggtgcgttg atgaggaata
    11701 tatcgatgcc atggtagata gagacaaaat gttatctgtt tatatgggga attttatcgc
    11761 gattcctcat ggaacagatg aagcaaaaaa atatgtgaaa aaatccggca tctgtgtcgt
    11821 tcaagtacca gatggtgtag attttggtac cgaagaagaa gaaaagattg cgactgtttt
    11881 gttcggtatt gcaggagtcg gagatgagca tttgcaactt gtacagcaaa tcgcacttta
    11941 ttgcagtgat atggacaatg ttgtccagct agcagacgcg ctcagcaaag aagaagtaac
    12001 ggaaaatcta gcaattgctt aaggagagag aaaaatgaga gcagtacatt ttggtgcagg
    12061 aaatatcggt cgaggattta ttggagaggt tttggcagct aatggatttg agattacttt
    12121 cgtagatgtg aatcacacga tcattgatgc tttaaatgaa agaaaagaat acgaaatcga
    12181 attagcagat gaatcaaaag aaaaaatcac tgtaaaaaat gtcaaaggaa tcaacaatca
    12241 aacaaatcca gaaaaagtaa tccaagaaat cgctgaagca gatttagtta ctaccgccat
    12301 tggcccgaat atcttacctt ttattgctga gttgattgca aaaggtatcc aagaaagaga
    12361 aaaggaagga agcacaacat ctttagatat cattgcctgt gaaaatatga ttggcggttc
    12421 agcatttcta gaaaaagaag tgtataaata tcttccggaa acatcattta ctgacaaata
    12481 tatcggcttt ccaaatgcag cagtcgatcg aatcgtccca ttacaacacc atgaagatcc
    12541 tttattcgtc caagtggaac ctttcaaaga atgggtcatt gacgatagcc aaagaaaaaa
    12601 taaaaaaatc caattaaaag gggttttata tgtagatgat ctagaaccat atatcgaacg
    12661 caaactattt agtgtcaata ctggtcatgc gactgtcgct tatacaggtg ccttgttagg
    12721 ttaccagacc attgatgaag caatgcaaga tgcgttagtt gtggcacaat taaaatcagt
    12781 tttgcaggaa acaggaagtt tgctgattgc caaatggggg tttgatgcag agcagcatca
    12841 tgcgtatatt gaaaaaatca ttcatcgctt ccaaaacaaa aatatctctg atgcgatcac
    12901 acgtgtcgct cggacaccat tacgtaagtt aggttatcaa gaaagattta cacgtccagt
    12961 gagagaacta caagaacata acttgacttg cccacatttg acggcaacaa tggggattat
    13021 ttttaattac tatgatcctg aagatgaaca aagccgtcaa ttgcatgaaa tgaagatcca
    13081 tgaaaacttg gaacaattga ttcaagaagt cacaggaatc aatgatccga aaacaatcgg
    13141 gaatatcaag caaaacgtga accgttatgc caagcaagtc gcttaaaata ggatacgaaa
    13201 aaggggaatg tgacgaaatt ttt
//
LOCUS       DACEEZ010000079        13001 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20313_2017
            SAMN12639966-rid9879223.denovo.079, whole genome shotgun sequence.
ACCESSION   DACEEZ010000079 DACEEZ010000000
VERSION     DACEEZ010000079.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639966
            Sequence Read Archive: SRR10023158
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13001)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13001)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741683.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741683.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:44:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,016
            CDSs (total)                      :: 2,962
            Genes (coding)                    :: 2,889
            CDSs (with protein)               :: 2,889
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 36 of 73
            Pseudo Genes (incomplete)         :: 29 of 73
            Pseudo Genes (internal stop)      :: 18 of 73
            Pseudo Genes (multiple problems)  :: 8 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13001
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639966-rid9879223.denovo.079"
                     /isolate="E20313_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            171..1307
                     /locus_tag="IWO89_002248"
     CDS             171..1307
                     /locus_tag="IWO89_002248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HAQ0456966.1"
                     /translation="MKTIYLDHAATTPMHPQVIEAMTAIMQDTFGNPSSIHGFGRHAH
                     EKLETARQIIADSLQAKPHEIIFNSGGTEGDNTAILETAFSRQKEGRHLITTAIEHPA
                     VLNTMKYLETKGFDVTYLPVDEKGNLSIEEVKKALREDTILVSVMYGNNEIGNLMPIK
                     EIGELLADHPAYFHTDAVQAYGNQTIHPEELGIDLLSISAHKINGPKGVGFLYKRDGV
                     NIPALLLGGEQEEKRRAGTENLAGIWGMAKAVELLSPEEREARNNKYKGFQQLILDTL
                     EANEIPYQVNGDLTNKLPHVLNLRLIGEANDLLLMKLDLNGVAISTGSACTAGNIEPS
                     HVLEAMYGEDTPILKESIRISFGLGNTEDDIQYFLDQLVRAVKK"
     gene            1444..1788
                     /locus_tag="IWO89_002249"
     CDS             1444..1788
                     /locus_tag="IWO89_002249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1831 domain-containing protein"
                     /protein_id="HAQ0456967.1"
                     /translation="MAFETTATVLGAPISYRLHPDAKRYTLRDNGFTETNGGNYQLIR
                     PLDATPQSKEGFKLKITVSKDIQTLKMSITTANGLKAVNIFKDPKQKMSQDKFYFLMD
                     GMISRGLFEKVE"
     gene            2059..3345
                     /gene="efmA"
                     /locus_tag="IWO89_002250"
     CDS             2059..3345
                     /gene="efmA"
                     /locus_tag="IWO89_002250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter EfmA"
                     /protein_id="HAQ0456968.1"
                     /translation="MENEQSVVLTNWKRNYLFFLSGQFLSGITSMVVQYAIIWYLTRE
                     TGSATILSFATLLGMIPMVLLSPFVGPLVDRWDKKALLIVTDIIVAIFALILAVVGTI
                     SESFPIWLVFVSLFMRSVAQTFQMPTIQSIMPTIVPSSHITRTNGQLGMVQSANFIIA
                     PALGAALFSVVPVNYLILLDVLGAVFGVGLLIFVKIPKVSPEILEVPLTIFKDAKFGL
                     QQLMDNKGLWYITINGAFVMLLFMPAISLYPLMTLDYFGGSVGQAGAVEVVYAVGMLL
                     GGALISFIGTWKDRMKPIIIAYIIMGLTIGASGLVPNDSQGFLYFLILNAGAGCATPY
                     FNTLLMAMIQQSYESNVLGRVLGNFNSLMNLTGPIGLLFAGPLADRLGVEKMFLFSGI
                     GILLCGIVLFLTSAARNYDKELQKKLVKEHHEQKDE"
     gene            complement(3483..4082)
                     /locus_tag="IWO89_002251"
     CDS             complement(3483..4082)
                     /locus_tag="IWO89_002251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphatase"
                     /protein_id="HAQ0456969.1"
                     /translation="MNYFISDMHFFHERLLGKNDFAPRLFANVDEMNQQLITSWNAVI
                     KETDHVYHLGDLAMHPQYEKANAEILELVTQLQGTIHLIKGNHDSRAFFRYLEKNDPR
                     LTDGSPKFYFYDVGAIVKFDHQQYYLTHYPMLLGPNDTIRNLHGHIHHYSVPIENDVN
                     VGVDAPERELLKEKLPFGTPLSEQQIKEIYEAKRKYDRS"
     gene            4486..5607
                     /gene="mnmA"
                     /locus_tag="IWO89_002252"
     CDS             4486..5607
                     /gene="mnmA"
                     /locus_tag="IWO89_002252"
                     /EC_number="2.8.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HAQ0456970.1"
                     /translation="MTDNSKTRVVVGMSGGVDSSVTALLLKEQGYDVIGIFMKNWDDT
                     DENGVCTATEDYKDVAKVAAQIGIPYYSVNFEKEYWDRVFEYFLAEYRAGRTPNPDVM
                     CNKEIKFKAFLDYAMDLGADYVATGHYAQVTRDENGVVHMLRGVDNNKDQTYFLSQLS
                     QEQLSKTMFPLGGMEKSEVRAIAERAGLATAKKKDSTGICFIGEKNFKQFLSNYLPAK
                     KGNMVTLDGEVKGQHAGLMYYTIGQRQGLGIGGGGDSQEPWFVVGKDLATNTLYVGQG
                     FHHPALYATSLDASEIHFTTNEPMPKEFKCTAKFRYRQQDVPVTVRLLDDNRAEVVFD
                     EPVRAITPGQAVVFYDGMECLGGGLIDHAYQETKVLQYV"
     gene            5853..7274
                     /gene="arcD"
                     /locus_tag="IWO89_002253"
     CDS             5853..7274
                     /gene="arcD"
                     /locus_tag="IWO89_002253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine-ornithine antiporter"
                     /protein_id="HAQ0456971.1"
                     /translation="MEKKSTGISKWGLVALVVSSSIGSGVFGITSDLASSAAPGPAIL
                     SWIIVGIGILALVLSLNHLGEKRPDLDGGIFGYAEASFGKLGGFISGWGYWLSAWLGN
                     VAFATMLMSAMGEFFPIFKGGQNVPSILFASIFIWGLTLLVNNGVEGASVINTIVTIC
                     KLVPLFLFLIFVFIAFKVNIFTADFWGNVSDNLVNGTGQQGTIWLQIKGCLMVMMWVF
                     VGIEGASVLANRAKKRSEAQQASIIGLLCLLFIYILASILPYGVLSQEELATISQPAM
                     ANILKGIVGNWGAALINIGLIISIIGSWLSWTMLPAETTMLMARDGLLPKGWGKTNKK
                     NAPTYSLVVTAILTNLFLLTFLVTDYAYQFAYSLCTAAILICYLLVGIYQVQFSLKEG
                     IKKQVVIGGIAVVFELMGIILAGFSYVLLCSIAYLPGFCFYWIACRETKHQITGKEKV
                     MIGLICVAALLSIGLLAVGWIKI"
     gene            7288..8406
                     /locus_tag="IWO89_002254"
     CDS             7288..8406
                     /locus_tag="IWO89_002254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase"
                     /protein_id="HAQ0456972.1"
                     /translation="MKIANFGVEEWLNKWEKKAVYDIAQSSIEALTLDELVGLDGTSV
                     SKFFEKRKNEPLDYGWIEGSDVFKQEVASLYQTVDPENILQTNGATGANLLALYALVE
                     AGDHVVSMLPTYQQLYDIPKSLGAAVDFVHLKEEEDWQFDLEQLEALVKPETKIICLN
                     SANNPTGTLLDRKTLEKIAEIARTVDAYVLIDEVYAPLTDKGEFLSIVDVYEKGIATN
                     SLSKTYSIPGIRIGWTATGPELAEVFRKYRDYTMICGGVLSDDLAVHALKNKEKILAR
                     NKKIITENLVILKQWVANEPKVELVAPNYVSTSFIKLAIEEDDQTFCINLLEETGVLL
                     VPGSAFDLPKHARLGYCCKKETLEKGLFLLSEFLKKQA"
     gene            complement(8466..9758)
                     /locus_tag="IWO89_002255"
     CDS             complement(8466..9758)
                     /locus_tag="IWO89_002255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter"
                     /protein_id="HAQ0456973.1"
                     /translation="MAARIKEKLFRKRETWEQNLFVLWFGTFMAGIGFSLVMPFMSLY
                     IDTLGNYSTSQLNFWSGLTFSSTFLVTTLISPWWGKLADQKGRKLMLLRASLGMAIVI
                     SLMGCVTSVYQLVALRLLQGIFSGYISNATALVATGTPKEKSGQVLGTLATGSVTGTL
                     LGPLLGGISASAFGYRPTFFITGIILFFVFLLSLFFVHEKFVPVKKENMVSAKQIFKD
                     LKYPHVVIGMFVTTMIIQASNNSISPIISLYIRQLLHGHGNVTLISGIIASIPGIATL
                     IAAPRFGRLGDKIGSERILAIGLGFAILVYIPMAFVTNVWQLAALRFLIGISDACLLP
                     AVQALITKYSPHQAAGRIFSYNQSFQATGNVVGPMIGSSVSSAFGYRGVFLSTSLLVL
                     VNFLLVRHNTQEIHQQETAEEKASGVSIASGHSPSHRQ"
     gene            9908..10468
                     /locus_tag="IWO89_002256"
     CDS             9908..10468
                     /locus_tag="IWO89_002256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HAQ0456974.1"
                     /translation="MKKRNLSQEKIIDCFRELAEEMDVQQITFQHLAKRLDIKAPSLY
                     NHFKNIRDVKTALTAKLLQELNDQLRRALVGKSGQEALQIYAETYRSFAFSNQAVYEL
                     LISVPHTNEAVLLDGIHETNQIILQIFEAFQLTKEEKVHRSRELRSMIHGYLSLRFLG
                     YFTKEPTIDPEDSYFWMINNFIATLP"
     gene            10597..10974
                     /locus_tag="IWO89_002257"
     CDS             10597..10974
                     /locus_tag="IWO89_002257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1033 family protein"
                     /protein_id="HAQ0456975.1"
                     /translation="MYQVITMFGDNEPWWFFEDWEEDIEEEETFESFEEARQAYEKQW
                     NEIHQNYEYINAKSNFLSAFWNDGDERWCEECDDDLQQYKGLALLKNHQPITVESRKE
                     FYETTNSSGKTKRCERPKQGAWC"
     gene            10907..12979
                     /locus_tag="IWO89_002258"
     CDS             10907..12979
                     /locus_tag="IWO89_002258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HAQ0456976.1"
                     /translation="MKQLILAEKPSVAKDLSKVLGANQKHKNYYEGPKVIVTWALGHL
                     LGLKMPEDLNKEWQSWQMDTLPMIPKKLGIKPLPKTGHQLKAIKQLAQRKDVSEAVIA
                     TDAGREGELVARWILEYVHFNKPVKRLWISSQTDKAIKTGFKQLRPAKEYDALYESAL
                     ARAKADWLVGLNVTRALTVKYADNLSAGRVQTPTLALVRDQEKKIEQFRPQTYFTITL
                     QAGKEQAKMLQKNQFALKTQEEAQKLVQLISTSKGTVTEVQEKLKTESAPLPYDLTEI
                     QREANQRYGYSAKKTLSLVQSLYETHKIVTYPRTDSKYLTNDMKSTMKERLQAVSDFA
                     PEVKQYLKNGAIVQQTKVFQDSKVTDHHALLPTENRARYEKLSNEERKIYQMIVSRFL
                     GLFAQPHKVSQTKVTVEFGKERFIFRQNRVVQAGWKGETETETETVKWEKGMRINPDF
                     AIKKELSAPPKPLSEASLLGQMEKYSLGTPATRAEIIEKLIKSELMERTPQGLQVSPK
                     GKQLLVLVNPSLVTPDLTESWEKDLEAIAAGKKQAGTFLNGIEKETKRLVNEIKSSKQ
                     EYQDFSITQKKCPECGANLREKNTRDGKIYVCTNSDCNYRRRKDPKVSNHRCPQCHRK
                     MEIIEGKNGAYFRCKYDGTTEKLLDKKEQKKKMTKHEERRLLKKYAQAEEPEESPLAA
                     ALKAAMKK"
BASE COUNT         4236 a         2327 c         2801 g         3637 t
ORIGIN      
        1 gaagttgtga cagaagtgag cagcttcaag gagctgcgac aagacttttg tcacagctcc
       61 ttttttctat gcccatgaaa cgtcctctag acaaaaatga gctcttcttt ggtttgttcg
      121 ctattttatg ctagaatgtg cctagcaata atttggaagg gatgaatgaa gtgaaaacta
      181 tttatctgga tcatgcggca actaccccca tgcatccaca agtaattgaa gcgatgacgg
      241 caatcatgca agacacgttt ggaaatcctt caagcataca tggatttggt cgccacgcgc
      301 atgaaaaatt ggaaacagca agacaaatca ttgcagacag tctgcaagca aaacctcacg
      361 agatcatctt taacagtggg ggaacagaag gggataatac agctatctta gaaacagcgt
      421 tctcgcgtca aaaagaaggc agacatctaa tcactacagc tatcgaacat cctgctgttc
      481 ttaataccat gaaatattta gagacaaagg ggtttgacgt tacatatctg cctgtagatg
      541 aaaaagggaa tctgtcgatc gaagaggtca aaaaagcgtt acgagaagat acgatcttgg
      601 tctctgtgat gtacgggaac aatgaaatcg gaaatttgat gccaatcaaa gaaatcggtg
      661 aacttttagc ggatcatcca gcttatttcc atacagatgc ggtacaagcg tacggaaacc
      721 aaacgatcca tccagaagaa ttaggaatcg atttattaag tatctctgct cataaaatca
      781 atgggccaaa aggcgtcggt ttcttatata aacgagacgg agtaaatatc cctgcattgc
      841 ttttaggcgg ggagcaggaa gaaaagcggc gtgcaggtac agaaaattta gcagggatct
      901 ggggtatggc aaaagcagtt gaactgctgt cacctgaaga acgtgaagca cgtaacaaca
      961 aatataaagg attccagcaa ttgattcttg atacattaga agcaaacgaa atcccttatc
     1021 aagtaaatgg tgatttaacg aataaactcc ctcatgtgtt gaatcttcgc ttaattggcg
     1081 aagcaaatga tttgctactg atgaagcttg atcttaatgg ggtcgctatt tctactggtt
     1141 cagcttgtac agcaggaaat atcgagcctt cacacgtatt ggaagcaatg tatggagaag
     1201 atacaccgat actaaaagaa tccatccgta tcagctttgg tttgggaaat accgaagacg
     1261 acatccagta tttccttgat caattagttc gtgctgtcaa aaaataacta gtgaagtcat
     1321 tcttatatga acagatgtct gagagaacgc cttcattttt tagggaactt aactgaaaaa
     1381 cctagtaaaa atcaggataa acttttataa taaagagaaa ctaattttat cgaggtgaaa
     1441 atcatggcat ttgaaacaac agcaacggta ctgggcgcac caattagcta tcgtttgcat
     1501 cctgatgcaa aaagatatac attgcgcgat aatggattta cagaaacaaa cggaggcaat
     1561 tatcaattga tccgaccatt agatgccacg ccgcaaagca aagaaggatt caaattaaaa
     1621 atcactgtat caaaagatat tcaaacattg aaaatgtcga tcactacagc aaatggattg
     1681 aaagcagtca atattttcaa agatccaaaa cagaaaatga gccaagataa attttatttt
     1741 ctgatggatg ggatgattag ccgtgggtta ttcgaaaaag tcgaatgatc tgtggatata
     1801 aaaagacaac tagttttgaa acggcctatt gttagatgac taactttgta aggacgacgc
     1861 aaattagttg tcttttttat ttttaaattt aacacatgtt gttattttta atattttgct
     1921 gatttattgt ttctaagtaa agaaatttaa attgaaacga ttgttattta gggaaaaagg
     1981 tattttttga aaaaagaatc ccgtataatg attgttgtgc aaacgctata caaaatgaaa
     2041 aagaaaagag ggatgtttat ggaaaatgaa cagtcggtcg ttttaacgaa ttggaagaga
     2101 aattatttgt tctttttatc gggacagttt ttgtcaggaa tcactagtat ggtcgtccaa
     2161 tatgcgatca tctggtattt gacaagagaa accggttcgg cgacaatttt gagttttgct
     2221 actttactag gaatgattcc catggtttta ctaagcccat ttgtaggacc gctagtggat
     2281 cgctgggata aaaaagctct tttgattgtc acggatatta ttgtagcgat tttcgcgctt
     2341 attttagcag tagtcggaac gatttcagaa tccttcccga tttggctagt ttttgtgtct
     2401 ttgtttatgc gttcggtcgc acaaacattc caaatgccaa cgatccagtc gattatgccg
     2461 acaatcgttc catcttctca cataacaagg acgaatggac agttaggaat ggtccaatca
     2521 gcgaatttca tcattgcgcc agctctaggt gcggctttgt tttcagtcgt tcctgtcaat
     2581 taccttattt tattagatgt attgggggca gtttttggtg tcggcttatt gatttttgtg
     2641 aagatcccaa aagtttctcc tgaaatattg gaagttccgc ttactatttt taaagatgca
     2701 aaatttgggc tgcagcaatt gatggataat aaagggttat ggtacataac gatcaatggt
     2761 gcatttgtga tgctgttgtt catgccagca atcagtctgt atccattgat gacgctagat
     2821 tactttggtg gttccgttgg acaggcggga gcagtggaag tagtctacgc agtcggaatg
     2881 ctcttaggcg gtgcactgat cagttttata ggaacatgga aagaccgaat gaaacctatt
     2941 attatagcct acatcattat gggcctgacg atcggtgcaa gcggattggt tccaaacgat
     3001 agtcaagggt tcttgtactt tcttatcttg aatgcaggag caggttgtgc aacgccatat
     3061 ttcaatacac tgctgatggc aatgatccag cagagttatg aatcgaatgt tttaggtcgt
     3121 gtcttaggga acttcaactc tttgatgaac ctgacaggtc caattggatt attatttgca
     3181 ggaccattag ctgatcgttt aggggtagaa aaaatgttct tgttttccgg aatcgggatt
     3241 ttattatgcg gaatcgtttt atttttaacg tcagctgccc gaaattatga taaagaatta
     3301 cagaaaaaac tggtaaaaga acaccatgag caaaaagacg aatagtatgg aatggagttg
     3361 tgacagaagt cttgttacaa ctccatttct gatcctaatg ttcctgtggc aataagccta
     3421 ctcaaatgaa aaatatgagt aattatcttt atttgtttaa tacttgatat agggcagttt
     3481 tttcacgacc tatcgtactt cctctttgct tcatagattt ctttgatttg ttgctcagat
     3541 aacggagtgc caaatggtag tttttccttt aagagttctc tttccggtgc gtctacgcct
     3601 acattgacat cgttctcgat aggtacgctg taatgatgga tatgtccatg aagattgcgg
     3661 atagtgtcgt taggcccgag caacatcgga taatgagtca agtaatattg ttgatggtcg
     3721 aatttaacga tggcacctac atcataaaaa taaaatttgg gacttccgtc agtaagccta
     3781 ggatcatttt tttctaaata acggaaaaaa gcgcgactgt catggttccc tttgatcaag
     3841 tggattgttc cttgcagttg agtgaccagt tccaaaattt ctgcatttgc tttttcatat
     3901 tgcggatgca tagcaaggtc gcctagatga taaacatggt cggtttcttt tattactgca
     3961 ttccagctag tgatcaactg ttggttcatt tcatccacat ttgcaaagag ccgcggagca
     4021 aagtcgtttt ttcctaataa gcgttcatgg aaaaagtgca tatcactgat aaagtaattc
     4081 atgtatcttt tgcctccctt ttttcttaga gtaaacaaaa tcacttatct gagcaactca
     4141 taacatctaa aaagaaaagg ttgtaaatga gacgtgcagc tttaagcgat aagaagcaat
     4201 tttgaaaaat agtttttcac aatttataaa attattcctt attgccgaaa ggctggccct
     4261 ttttatgagt atgccaattt tttatatcga atgctcaaaa gatgagtgat tttttggttg
     4321 actatcatgt ttgtgtactg aataggtcca gtagaacagt atttttacga cttgataatt
     4381 aagggatgaa attttcagaa atatgacgta taatgtaaaa tactgaaaag ttgcgacctt
     4441 caactcgtag atgtttcaga atctaattga aatgatggtg atatcatgac agacaacagc
     4501 aaaacacgcg ttgtcgtagg gatgagcggc ggtgtcgact catctgtaac agcacttttg
     4561 ttaaaggaac aaggatacga cgtgatcgga atattcatga aaaattggga tgacacggac
     4621 gaaaacggtg tatgcacggc cacggaagat tataaggatg tagcgaaagt agctgctcaa
     4681 atcgggattc cttattactc tgtgaatttt gaaaaagaat attgggatcg tgtatttgaa
     4741 tatttcctag ccgaataccg cgcaggacgt acacctaacc cagacgtgat gtgcaacaaa
     4801 gaaatcaaat tcaaagcatt cttggattat gcaatggatc ttggcgccga ttatgtagct
     4861 actggacatt atgcgcaagt cactcgcgat gaaaacggcg tggtccacat gcttcgtgga
     4921 gtagacaata ataaagatca aacttacttc cttagccaat tatcacaaga acagctttca
     4981 aaaacgatgt tccctctagg cggcatggaa aaatcagaag tacgggcaat tgctgaacgc
     5041 gcaggccttg cgactgctaa gaaaaaagat tcaacgggaa tctgctttat tggtgaaaag
     5101 aacttcaaac aatttttgag caactatctg ccagcaaaaa aaggaaatat ggtcactctt
     5161 gatggtgaag tcaaaggaca acatgctggc ttgatgtatt atacgattgg tcaacgtcaa
     5221 ggactaggga tcggtggcgg aggagattcg caagaaccct ggtttgttgt aggaaaagat
     5281 ctagcgacaa acacattata tgtggggcaa gggttccatc atcctgcatt gtatgccaca
     5341 agcttggatg caagtgaaat ccactttacg acgaatgaac caatgccaaa agaattcaag
     5401 tgtacagcga aattccgtta tcgtcagcaa gacgttcctg tgacagttcg cttattggat
     5461 gataatcgtg cagaagtcgt attcgacgaa cctgtacgtg caatcactcc tggacaagca
     5521 gttgtatttt atgatggaat ggaatgtctg ggtggtggtt tgatcgacca cgcatatcaa
     5581 gaaacaaaag ttttacaata cgtttaagcg atattaaatg aatcaaaaca gctatggcta
     5641 acaaaagaaa caccttttct tttggatagc atagctgttt ttttgcaata aaattaattt
     5701 ttattcattt tatacttgaa atacttgtgt aaaaagttca taatatccaa taatatgaat
     5761 aaagttgctt tttgttaagc aagagcagtt gcaaaataga agggaatctt ttgcaagtga
     5821 tttgttcaat tttataagtg agggtgatgg aaatggaaaa gaaatcgacg ggtattagca
     5881 aatggggact ggttgctctg gttgtcagtt cgtcaatcgg aagcggtgtg tttgggatca
     5941 caagtgatct ggctagttct gcagctccag ggccagcgat cctttcatgg ataatcgttg
     6001 ggattggtat tttagctttg gtattatcgc tgaatcattt aggagaaaag cgtccagatc
     6061 tagatggtgg tattttcggt tacgcggaag cttcatttgg taaattaggt ggcttcatca
     6121 gtggttgggg ttactggttg tctgcgtggc tgggaaatgt tgcttttgca acgatgctga
     6181 tgagcgccat gggagaattt ttcccgatat tcaaaggtgg acaaaatgtt ccttctattt
     6241 tatttgcaag tatttttatt tggggtctga ctttattagt aaacaatgga gttgaaggag
     6301 cgagcgtcat caatacaatc gtgacgatct gtaaattagt gccattattc ctatttttaa
     6361 tatttgtatt tatcgcattt aaagtgaata tattcactgc tgatttttgg ggtaacgtat
     6421 ctgataatct agtgaatgga acagggcagc aaggaacgat ctggttgcag attaaaggtt
     6481 gtttgatggt tatgatgtgg gtctttgtag ggatcgaagg cgcaagtgtt ttagccaacc
     6541 gcgcgaagaa acgttcagaa gcccagcaag catcgatcat tggtctgctt tgtttattgt
     6601 ttatctatat ccttgcttcg atcttgccat acggcgtctt atctcaagaa gagttagcaa
     6661 ctatttcaca acctgctatg gcaaatattt taaaaggaat cgtgggaaat tggggagcag
     6721 cgctgatcaa tatcggcttg attatttcga tcattggttc ttggttgtct tggacgatgc
     6781 tcccagcaga aacaacgatg ttgatggctc gagacggtct gttgccgaaa ggttggggaa
     6841 aaacgaataa aaaaaatgcc ccaacttatt cgttagttgt tactgcaatc ttgactaatc
     6901 tattcttatt gactttctta gttactgatt atgcctatca atttgcctat tctttatgta
     6961 cagcggcaat tttgatttgt tatctacttg tcgggatcta tcaagtccag ttttctttaa
     7021 aagaaggaat caaaaaacaa gtagtgatcg gtgggattgc tgtagttttt gaattgatgg
     7081 gcatcatttt agcaggattt tcttatgtcc ttttatgtag tattgcctat cttcctggtt
     7141 tttgcttcta ctggatcgct tgtcgtgaaa ccaaacatca aattacggga aaagaaaaag
     7201 taatgattgg gctgatttgt gtggccgcac tgctttcaat cgggttgctt gctgtaggct
     7261 ggatcaagat ataaggggga aagaaacatg aaaatcgcaa atttcggtgt agaagaatgg
     7321 ctaaataaat gggaaaagaa agcggtatat gatattgcac aaagctcgat cgaagcattg
     7381 acattagacg aactcgttgg cttagatggg actagcgtaa gtaaattttt cgaaaaacgc
     7441 aaaaacgaac cattggacta tggctggatc gaaggatcag atgttttcaa acaagaagtc
     7501 gcttctcttt atcaaaccgt cgatccggaa aacatcttac aaacgaatgg agcgactggt
     7561 gctaatcttt tagctctata cgcgttagtg gaagcaggag accatgttgt ttcgatgctt
     7621 cctacttatc agcaattata tgacatcccc aaatctttag gtgccgctgt tgactttgtt
     7681 catttgaagg aagaagaaga ttggcagttt gatttagaac agctagaagc cttagtcaaa
     7741 cctgaaacaa aaatcatctg tttgaatagt gcaaacaatc ctacaggcac attgctagat
     7801 cggaaaactt tagaaaaaat cgcagaaatt gctcgtacag tagatgcata tgtattgata
     7861 gatgaagtat atgcgccttt aacggataaa ggtgaattct tgtcgatcgt agatgtctat
     7921 gaaaaaggta tagcaaccaa ttccctatcc aaaacctact ccattccagg tattcgtatt
     7981 ggctggacag ctactggacc tgagttggca gaagtttttc gaaagtatcg agactacacg
     8041 atgatttgcg gcggagtcct gtcagatgat cttgctgtac atgcattgaa gaataaagaa
     8101 aaaatattgg cgcgaaacaa aaagatcatt acagagaacc ttgtgatttt aaaacaatgg
     8161 gtagccaatg aaccgaaagt tgaacttgtc gctcccaatt acgtctctac ttctttcatt
     8221 aaattagcca tcgaagaaga tgaccagaca ttttgtatca atttattaga agaaacagga
     8281 gtactattag taccaggaag tgcctttgat ttaccgaagc atgcccgact aggttactgt
     8341 tgtaaaaaag aaaccttgga aaagggatta ttcctcttat ctgagttctt gaaaaaacag
     8401 gcctaaacaa aaacactgca aggacagtct gtgacaactg ttcaagcagt gtttttcatt
     8461 tacgcttatt gtctatgaga aggagagtgc cctgaagcta tggagactcc tgaagctttt
     8521 tcttctgcag tttcttgctg atggatctct tgtgtattgt gtcgtacaag caagaagttg
     8581 accaaaacaa gaagagaagt cgaaagaaag acgccacgat aaccaaatgc actggaaaca
     8641 ctggagccga tcataggccc tactacgtta ccagttgctt ggaaagattg gttataactg
     8701 aagatccgtc cagcagcttg atgaggcgaa tattttgtga tcaatgcttg gacagcaggg
     8761 agcagacaag catcagagat accaatcaaa aaacgtaagg cagctaattg ccaaacattc
     8821 gtgacgaatg ccattgggat ataaaccaga atcgcaaaac ccaaaccaat cgcaagtatt
     8881 ctttcactcc caatcttgtc acctaagcga ccaaatcttg gagcggctat caaagtggca
     8941 atcccaggta tcgaagcaat gattccgctg atcaacgtaa cattcccatg tccatgcagc
     9001 aattggcgaa tgtacagact gatgattgga ctaatggaat tgttggatgc ttggatgatc
     9061 atcgttgtga cgaacatacc gatgacgacg tgtggatatt tcaagtcttt gaaaatctgt
     9121 ttggcagaaa ccatgttttc ttttttcact gggacaaatt tttcatggac gaaaaacaga
     9181 ctcaacaaga agacgaaaaa taagatgatc cctgtaatga aaaaggtagg gcgatagcca
     9241 aacgctgaag cagagatccc gccaagaagc ggtccaagta atgttcctgt aacagaacca
     9301 gttgctaagg ttccaagaac ttgaccactt ttttcttttg gcgtcccagt tgcgacaagg
     9361 gcagtggcat tactgatata accagaaaaa atcccttgaa gcagacgcaa tgcgactaat
     9421 tgatacacac tagtcacgca acccatcaaa gaaatgacga ttgccattcc taatgaagca
     9481 cgcaacagca tcaattttct tcctttttgg tcggctaatt ttccccacca aggagagata
     9541 agcgtagtca ccaagaaagt agatgaaaaa gtcaatccgc tccaaaagtt caattgtgac
     9601 gtactataat tacctaacgt atctatgtaa agtgacataa aaggcatgac taaactaaat
     9661 ccgataccag ccataaatgt tccaaaccat aagacaaaca agttttgttc ccaagtttct
     9721 cttttcctaa ataatttttc ctttattctt gcagccaatt agactccttc tttctgaaaa
     9781 ttttcatcta cagctcttta ttataaacta caatatgtag tttatcaatg aaagtctctt
     9841 ttttctcttt ctttttcgtg gtaaactgaa agagtgaagg atttatttta tgagaggaaa
     9901 catggaaatg aaaaaaagaa atctgtctca agagaaaatc atcgactgct ttcgagaact
     9961 agcagaagag atggatgttc agcaaataac ctttcagcat ttagccaaac gcttggatat
    10021 caaggctccc tcgctgtata accatttcaa aaatattcgt gatgtcaaaa cagcgctaac
    10081 agcgaaactt ctgcaggaac ttaatgacca gctgcgacgg gctcttgtgg gaaaaagtgg
    10141 tcaggaagca ctgcagatct atgcagaaac ttatcgatcc tttgcttttt caaatcaagc
    10201 agtgtatgag ttactgatca gtgtaccgca taccaatgaa gctgttttat tagatggaat
    10261 ccatgaaacg aatcagatca ttttgcagat atttgaggct tttcaattaa cgaaagaaga
    10321 aaaagttcat cgaagcaggg aacttcgtag tatgatccac ggttatcttt ccttacgttt
    10381 tttaggatac tttacaaaag aaccaacaat cgatccagaa gacagctatt tttggatgat
    10441 caataacttt atcgctactt tgccttaaac aatcagattc atttgtaaca attttaagat
    10501 gttcattaaa tgaccctttt ataagagaaa attcttgcaa ctttcttgaa aagtgactaa
    10561 gatgagtcta gaaaagctga aagaaggagc acgagtatgt atcaagtaat taccatgttc
    10621 ggggacaatg agccttggtg gttttttgaa gactgggaag aagatattga agaagaagaa
    10681 acattcgagt ctttcgaaga agcacgacaa gcgtacgaaa aacaatggaa cgaaatccat
    10741 caaaattatg aatatatcaa tgcaaaaagt aactttctca gcgccttttg gaatgacgga
    10801 gatgagcgct ggtgtgaaga gtgtgacgat gatttacagc aatacaaggg acttgcgttg
    10861 ttgaaaaatc atcagcccat cacagtagaa agcagaaagg aattttatga aacaactaat
    10921 tctagcggaa aaaccaagcg ttgcgaaaga cctaagcaag gtgcttggtg ctaatcaaaa
    10981 gcacaaaaat tattatgaag gacccaaggt gatcgttact tgggcccttg gtcatttatt
    11041 aggattgaaa atgccggaag atctgaataa ggaatggcag agctggcaaa tggacacgtt
    11101 gccaatgatc cctaaaaaac taggaatcaa accacttcct aaaacaggac atcaattaaa
    11161 agcaatcaag cagctggctc agcgaaaaga tgtctcagaa gcagtcattg cgacagatgc
    11221 cggaagagaa ggggaacttg ttgctcgctg gatcttagag tatgtgcatt tcaataaacc
    11281 agtgaagcgg ttatggatct cttctcaaac agataaagca atcaaaactg gattcaagca
    11341 actaagacct gcgaaagaat atgatgcatt gtatgaatct gctcttgccc gggcaaaagc
    11401 ggactggctg gtaggattga atgtgaccag agccttaact gtgaagtacg cggataattt
    11461 atcagcaggc cgtgtgcaga caccgacact agcattagtg agagaccaag aaaagaaaat
    11521 cgagcagttc cgtccacaaa cgtatttcac gatcacgttg caagctggaa aagaacaagc
    11581 caaaatgctt caaaaaaatc aatttgcttt aaaaacacaa gaagaagcac aaaaacttgt
    11641 tcaattgatc agtacaagca aaggaactgt cacagaagtc caagaaaaat taaaaacgga
    11701 atctgcgcca ttgccttatg atttaacaga gatccaaaga gaagccaatc aacgctatgg
    11761 ctattctgca aagaaaacat taagtcttgt acaaagtttg tatgagaccc ataagatcgt
    11821 tacttatccg cgtacagaca gcaaatatct gacaaatgac atgaaaagta cgatgaaaga
    11881 acgattgcaa gcagtcagcg actttgctcc tgaggtcaag cagtatttga aaaacggagc
    11941 catcgtccaa cagacaaaag tattccaaga cagtaaagtg accgatcacc atgcactttt
    12001 gccaactgaa aacagagcac gttatgaaaa attaagtaat gaagagcgaa aaatctatca
    12061 aatgattgtc tctcgttttt taggactgtt tgctcaaccc cataaagtca gccaaacaaa
    12121 agttacggtt gaatttggta aagaacgatt catttttcgg caaaatcgtg tcgtccaagc
    12181 aggatggaaa ggcgaaactg aaactgagac ggaaacagtc aaatgggaaa aaggaatgag
    12241 aatcaatccc gatttcgcca tcaaaaaaga actatctgca ccgcctaaac cattgtcaga
    12301 agcaagcttg cttggtcaaa tggaaaaata cagtctggga accccagcta cgcgtgcaga
    12361 gattatcgag aaactaatca aatctgaact gatggaacgt acccctcaag gactgcaagt
    12421 atctcctaaa ggtaagcaat tattagtatt ggtaaatccc tctttagtaa caccagattt
    12481 gacagaaagc tgggaaaaag accttgaagc tattgctgca ggaaagaaac aagcaggaac
    12541 cttcttgaat ggaatcgaaa aagaaacaaa acgcttggtg aatgagatca aatcaagcaa
    12601 acaagaatat caagatttct cgattaccca aaagaaatgc ccagaatgtg gggccaatct
    12661 gcgcgagaaa aacacacgtg acggcaaaat ctatgtctgc accaattcag attgcaacta
    12721 ccgcagaaga aaagatccaa aagtctctaa tcatcgatgc ccgcaatgtc accggaaaat
    12781 ggaaatcatc gaaggtaaaa acggtgctta tttccgttgt aaatacgatg gaacgacaga
    12841 aaaattgctt gataaaaaag agcagaagaa gaaaatgacc aaacatgaag aacgacgctt
    12901 gctgaaaaaa tatgcacaag cagaagaacc agaagaaagc ccattagcag cagcactaaa
    12961 agcagcaatg aagaagtaat aagatatgaa agaggttgtg a
//
LOCUS       DACEEZ010000080        12946 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20313_2017
            SAMN12639966-rid9879223.denovo.080, whole genome shotgun sequence.
ACCESSION   DACEEZ010000080 DACEEZ010000000
VERSION     DACEEZ010000080.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639966
            Sequence Read Archive: SRR10023158
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12946)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12946)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741683.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741683.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:44:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,016
            CDSs (total)                      :: 2,962
            Genes (coding)                    :: 2,889
            CDSs (with protein)               :: 2,889
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 36 of 73
            Pseudo Genes (incomplete)         :: 29 of 73
            Pseudo Genes (internal stop)      :: 18 of 73
            Pseudo Genes (multiple problems)  :: 8 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12946
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639966-rid9879223.denovo.080"
                     /isolate="E20313_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            135..2606
                     /locus_tag="IWO89_002259"
     CDS             135..2606
                     /locus_tag="IWO89_002259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HAQ0456977.1"
                     /translation="MKNKWINRSKKKKVTQRSSDKVIRLFPSYKEGLTADQVKERIEK
                     GAANNSVDPTFKTNQQIVLENIFTYFNLIFLILAILLCLVESYKNLTFLPVIIANTGI
                     AIFQEIRSKKILDELNVLNTAKVNVIRDGIEQTIDIEDLVIDDIVILETGYQIPADAE
                     VVEGKLQVNEALLTGEADEITKEIGDPLMSGSFIVSGKAYARLDKVGADSYISQLTVK
                     AKTMGEGEQSEMIASLNQLIKWIGIIIIPIGILLFSQSYFFNGNTIKESVVAMEAALI
                     GMIPEGLYLLTTIALALSATKLAKQRVLLHNMKSIETLARVNVLCVDKTGTITENKMS
                     VQKMIVSKKQEIISQSDQLEACIADYAKAMAGDNATMEAVKAYFTKTTDQSYHHVIPF
                     SSVQKFSSVALADKVYVLGAPEMVLRDQLPEYAEEFVSFAEKGYRVLVFGVYDGILEE
                     PVLTEAVTPLGYILIANPIRKEARATFDYFKKQQVAIKVISGDNPLTVSNVAVQAGIS
                     HADQYVDASKLSEEEYEAAVEKYTVFGRVKPEQKKIFVQLIKRNNTVAMTGDGVNDIL
                     AMKEADCSIAMASGNEAAMQASQVVLLDSDFSRMPEVVAEGRRVVNNIERSASLFLVK
                     NIFSFLLSLFSVIFALTYPLEPSQITLISLFTIGLPSFLLALEENKKRIRGKFIMNVM
                     EKAVPGGLTDMIVVGALVICGVTLNLNKTDVSTASTMLLIAVGFLVLYKICSPLNKFR
                     SQIILFCASGNFFSVIFLHKLFSITGISAVSLLLLSILFFSADSIFRHLTTLVEYLFE
                     RKNEKEQKPFSRVAKKFLSIFKREN"
     gene            complement(2709..4445)
                     /locus_tag="IWO89_002260"
     CDS             complement(2709..4445)
                     /locus_tag="IWO89_002260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component sensor histidine kinase"
                     /protein_id="HAQ0456978.1"
                     /translation="MKWKKIYERLFFLILLVSLFFGGWQLISHYFQQQIVEQQESYLT
                     KKAQLLIRQLDVENLQAAQNKTALEEFVHQSNERITLMDATGKILFDTNNETLNEQRN
                     SRPEIKAVLNGGNLGSALRKSTTLNEDLLYVALPVKKSGQLEAILRIAEPTSGFLPRT
                     ESFRRWVFFFFLSFFLVLAGMIYYLIYQKNQPLKTVLPVLKKMVQNPNQAEVIMQTPD
                     QWEELYQTINALSEQMSKMYRACTTTEEQLYTLLNELMIGVFLIDDADSRLLLLNPKM
                     QLHLNVVSYQPEQRYTEVIQEPKLIQLIHQVTPEHPLIHQEITLTEGRQTLDLSLRYF
                     TNSEGTGQILGVAYDLTKVRRLEKLQKDFVGNVSHELKTPVTSLIGFTETLLDGAKDD
                     PQTLTSFLEIMQKDAIRLDKLIREIIQLSKDGENSYEIQTIYTEPYFQQIVQNYQPII
                     EKKRLTIRLIGKNEPFTTKTDILYPIIKNLIENAVQYSKSDSEIIIRYQATDDLSFSV
                     QDFGIGIDIEDQERIFERFYRVDKARSRHSGGTGLGLAIVKDYVQLLNGTITVDSHLG
                     TGSTFTVTIPKM"
     gene            complement(4442..5146)
                     /locus_tag="IWO89_002261"
     CDS             complement(4442..5146)
                     /locus_tag="IWO89_002261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAQ0456979.1"
                     /translation="MKKILVVDDEPSIVTLLTFNLEKEGYKVTSATDGGEGLELALEQ
                     SFDFIILDVMLPTMDGMEITQRLRQEKNETPILMLTAKDDQVDRIIGLEIGADDYLTK
                     PFSPREVLARMKAIFRRIEPRKQSKDEAEPEYLSIGQITADLTNYQVTVDDHLIELTP
                     KEFELLVYFMKRKDRVIDRDTLLDRIWNFDFAGQSRIVDVHVSHLREKIERDPKHPKY
                     LVTIRGFGYKFQEPKR"
     gene            5277..5780
                     /locus_tag="IWO89_002262"
     CDS             5277..5780
                     /locus_tag="IWO89_002262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF523 domain-containing protein"
                     /protein_id="HAQ0456980.1"
                     /translation="MIGISACLGGLACRYDGQKKEVPELMELVETGEAVMVCPEVIGG
                     LPIPRDPAEIIGGNGFDVWKDQAKVWTTSGEDVTEAYKNGAIKAYQKLQEKHIDLVIM
                     KEKSPSCGTHSIYDGSFSGIKKNGAGVATAYFIQQKMTVLSDEEWLKTRGEQNGNRKD
                     QSNERAL"
     gene            5740..6078
                     /locus_tag="IWO89_002263"
     CDS             5740..6078
                     /locus_tag="IWO89_002263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative DNA-binding protein"
                     /protein_id="HAQ0456981.1"
                     /translation="MEIEKTNRMNALFEFYSTLLTEKQMNYMELYYADDFSLGEIAEE
                     YEVSRQAVYDNIKRTSKILEDYEKKLHLFSNYIVREQVLENMTAYITDKYPEDKKLLE
                     YVQQIQAIEE"
     gene            6099..7517
                     /gene="ffh"
                     /locus_tag="IWO89_002264"
     CDS             6099..7517
                     /gene="ffh"
                     /locus_tag="IWO89_002264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HAQ0456982.1"
                     /translation="MAFESLTERLQQAMGKIRKKGKVSEADVKEMMREIRLALLEADV
                     NLQVVKDFTKRVRERAVGAEVLESLSPAQQIVKIVDEELTITLGSETAELNKSPKIPT
                     VIMMAGLQGAGKTTFTGKLANYLKKNENARPLLIAGDVYRPAAIDQLKVLGQQLDVPV
                     FDMGTEVSPVEIVRQGMELAKEKKNDYVLIDTAGRLHIDETLMDELKQIKELTQPNEI
                     LLVVDAMTGQDAVNVADSFNQQLGITGVVITKLDGDTRGGAALSIRSVTGAPIKFIGS
                     GEKLTDLEVFHPDRMASRILGMGDMLTLIEKAQQDYDEKKAEELAQKMRENSFDFNDF
                     IEQLDQVMGMGPLEDLIKMIPGMNQVPGIENVKVDPKDVERKKAMVYSMTPAERANPD
                     LLNPSRRRRIAAGSGNSVVEVNRMIKQFKESRKMMQQMSKGDMNIPGMDQMFGSGVKG
                     KLGKMAMNRMIKKNKKKKKKRK"
     gene            7629..8207
                     /locus_tag="IWO89_002265"
     CDS             7629..8207
                     /locus_tag="IWO89_002265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase I"
                     /protein_id="HAQ0456983.1"
                     /translation="MRCPWSEQTESMRQYHDTVWGVPEYDDQKLFRKLMLDINQAGLS
                     WQTILNKSEAFDEAYDRFDIKKVANYGETKISELMENKGIIRNRRKIEAAVQNAKAVL
                     KIQEEIGSFSEYLWSFSNGKIVDSSYHSQEEVPTTSPLSDELAKDLKKRGFKFIGSTT
                     IYAFLEAVGIINDHLDSCFRKEEVVRIGRKGE"
     gene            8211..8732
                     /locus_tag="IWO89_002266"
     CDS             8211..8732
                     /locus_tag="IWO89_002266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HAQ0456984.1"
                     /translation="MRKIIFYGAISLDGYLATKTDDLQWLFDTPTGKQTTYEAFYETI
                     DTTIMGRKTYQEAKKYLETEAIYPEKKNFVFSTHAQLQLPDAEVVNEDPVAFLKNLQM
                     TEGKDIWVVGGGRLIKPLLEHQMIDEWYIQITPVLLGDGIPLFQKGTYEARFEMLDTT
                     RFGEFIELHYVRK"
     gene            complement(8811..9365)
                     /locus_tag="IWO89_002267"
     CDS             complement(8811..9365)
                     /locus_tag="IWO89_002267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HAQ0456985.1"
                     /translation="MTKKIGFFIGSLRKDSYNKKVAEAFAKLLPEGYESVFVKIDDLP
                     FYNEDLETPEQAPAEWTRFREEVRGLDGVVFVTPEYNRSVPAVLKNALDVGSRPYGHS
                     VWDHKPGLVVTASPGGIGGFGANHHLRQSLVFLNVPTLQQPEAYIGNVANLIDENGNI
                     VEGTLSFFQTILDAYLDFSNKLTE"
     gene            9618..9893
                     /gene="rpsP"
                     /locus_tag="IWO89_002268"
     CDS             9618..9893
                     /gene="rpsP"
                     /locus_tag="IWO89_002268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016184541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HAQ0456986.1"
                     /translation="MAVKIRLKRMGSKKSPFYRIVVADSRSPRDGRFIETVGTYNPLK
                     DPAEVVLKEDLVLDWLSKGAQPSDTVRNILSKEGVMQKHHEAKFSKK"
     gene            9905..10156
                     /locus_tag="IWO89_002269"
     CDS             9905..10156
                     /locus_tag="IWO89_002269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KH domain-containing protein"
                     /protein_id="HAQ0456987.1"
                     /translation="MKDLSDLVLTIVRPLVTYPDQVKLDVVESKDFYEYNLTVAPEDI
                     GRIIGKQGRVAKAIRTIVYGVRINAPKKVRLNIIDDKEK"
     gene            10281..11072
                     /locus_tag="IWO89_002270"
     CDS             10281..11072
                     /locus_tag="IWO89_002270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0456988.1"
                     /translation="MRKKMLFIPFALLLSACSSVTQESDNTHLSSSISETVTSPEKSS
                     TKTASTTEQTMATSNNEKKTALDQLKEQQPNVPMPLDVPVSSGYLNIAATHTKQGYSI
                     LYYRTDRPLGLNADELNQETPIATYLYQYGFASSQETIQVLQPFEIDTNGQQVDLGSR
                     ITGYQQGAAGSSFLEWQEGNWRIRIRGNNIEGQDPLLLAKEIVAYLEENSLPAPEQFG
                     KITVDMGDTTNRAVEVSWQEPKNAYTITHQDPMSALKMAVSMKRL"
     gene            11242..11763
                     /gene="rimM"
                     /locus_tag="IWO89_002271"
     CDS             11242..11763
                     /gene="rimM"
                     /locus_tag="IWO89_002271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimM"
                     /protein_id="HAQ0456989.1"
                     /translation="MTEYLNVGKIVNTQGIKGEVRVISTTDFPEERYKKGTVLTLFQD
                     GKAPVELTVKSHRKHKNFDLLSFEGHPSINDVEKYRDGILKVSKDNSVDLAENEFYYH
                     EIIGLHVLEDGTELGKVKEILSPGANDVWVVQRPKKADILLPYIASVVKEVDLEAGVV
                     RVEIPEGLIDDED"
     gene            11753..12514
                     /gene="trmD"
                     /locus_tag="IWO89_002272"
     CDS             11753..12514
                     /gene="trmD"
                     /locus_tag="IWO89_002272"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HAQ0456990.1"
                     /translation="MKIDVLTLFPRMFEGPMGESIIGKAVDKGLLDINISNFRDYSDN
                     KHQTVDDYPYGGGAGMLLKVQPIYDNIQAIEKAAPDVKKRVILLDPAGKRFDQKMAEE
                     FSQEEHLVFICGHYEGYDERIRTLVTDEVSLGDYVLTGGELGAMVMIDATVRLLPDVL
                     GNQTSAQTDSYSTGLLEHPQYTRPAEYKGMKVPEVLTNGNHKLIEEWQLKESLRRTYQ
                     RRPDLLETLEWTPQMTKFLEEIKKEEQEKTADEAK"
     gene            12650..>12946
                     /gene="rplS"
                     /locus_tag="IWO89_002273"
     CDS             12650..>12946
                     /gene="rplS"
                     /locus_tag="IWO89_002273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002357176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HAQ0456991.1"
                     /translation="MNPLIEELTKEQLRSDIPAFRPGDTVRVHAKVVEGTRERIQLFE
                     GVVIKRRGAGISETYTVRKVSNGVGVERTFPLHTPRVAKIEVVRYGKVRRAKLYY"
BASE COUNT         4282 a         2370 c         2685 g         3609 t
ORIGIN      
        1 cctttttttt tgctatatga caggaatgaa aggtgtttgc aagcttaatg tgaggaacac
       61 tttccatatc tgttctatac tggataggag aagtttggaa atatccagaa aacgaaatca
      121 atcgaggtag ccacatgaag aataaatgga taaatcgaag taaaaaaaag aaagtaacac
      181 aaagatcatc agataaagtc atccgtcttt ttccatcgta taaagaagga ttaacggcag
      241 atcaagtcaa ggaacgaata gaaaaaggtg cagcaaataa ttcagtcgat cccacattca
      301 aaacaaatca gcaaatcgta ttggaaaaca tatttacgta tttcaatttg atctttctta
      361 tcttagccat tttgctttgt ttagtagaat cctataagaa cttgactttt ttaccagtta
      421 tcatagccaa tacaggaatc gccatttttc aggaaattcg atccaaaaag atcttggacg
      481 aactaaatgt tttaaataca gcaaaagtca atgtcatacg agacgggata gaacaaacaa
      541 tagatataga agaccttgta atagatgaca ttgttatttt agagactgga tatcaaattc
      601 cagcagatgc agaggtcgtg gaagggaaac tgcaagttaa tgaggcttta ttgacaggcg
      661 aagcagatga aataaccaaa gaaataggtg atccattaat gtccggcagt ttcattgttt
      721 ccggaaaagc ttacgcccga ttagacaaag ttggagcaga ttcgtatatt tcccagctga
      781 cagtcaaagc caagacaatg ggagaaggag aacagtcgga gatgattgct tctcttaatc
      841 aattgatcaa atggattggg atcatcatta ttcctatcgg tatcctgctt ttttctcaaa
      901 gttacttttt taatggaaac acgatcaaag aaagtgtcgt tgcaatggaa gcagcattga
      961 tcggtatgat acctgaagga ctgtacttgc tgacgacgat tgcattagca ttaagtgcga
     1021 ctaaactagc aaagcaaaga gttcttttac acaatatgaa aagcattgag acattggcaa
     1081 gggtaaatgt cctttgtgta gataaaacag gaacgatcac agaaaataaa atgtctgttc
     1141 aaaaaatgat cgtctctaaa aaacaagaaa taatcagtca gtcagatcaa ttggaagcat
     1201 gcattgctga ctatgcaaaa gcgatggcag gagacaatgc aacaatggaa gctgttaaag
     1261 catattttac aaaaaccact gatcaatcct atcaccatgt tattcctttt tcttctgtcc
     1321 aaaaattcag cagtgtagcg ttggcagaca aggtgtatgt tttaggagcg cccgaaatgg
     1381 ttttgcgcga tcaattgccg gaatatgcgg aagagtttgt ttctttcgct gaaaaaggct
     1441 atcgcgtgtt ggtctttggt gtgtacgatg ggattcttga agaaccagta ttgacagaag
     1501 cagtcacacc attaggctat attctgattg ctaatcctat cagaaaagaa gcaagagcca
     1561 catttgatta cttcaaaaaa cagcaagtag ccatcaaagt gatttctgga gataatccgc
     1621 tcaccgtatc caatgtagct gtccaagcag ggatttcaca tgctgatcag tacgttgatg
     1681 catctaaatt atcagaagaa gaatatgaag cagctgtcga aaaatataca gtttttggac
     1741 gcgtcaagcc agaacaaaag aaaatcttcg ttcaattgat aaagcgtaac aatacagtag
     1801 ctatgacagg tgatggcgtg aatgatattc tagccatgaa agaagcagac tgcagtatag
     1861 cgatggcttc cggaaatgaa gccgccatgc aggcctcaca agtggtctta cttgattctg
     1921 acttttcacg catgccagaa gttgttgcag aaggacggcg cgttgtgaac aatattgagc
     1981 gatcagccag cttgttttta gtgaagaata ttttctcctt tttgctctca ctgttttcag
     2041 tgatctttgc tctgacctat cctttagaac catcacaaat cacattgatt agtttgttta
     2101 ctatcggact tccatctttc ttgcttgctc tagaagaaaa caaaaagcga atcagaggga
     2161 aattcatcat gaatgtgatg gaaaaagctg tgccaggtgg actaacagat atgatcgtag
     2221 tcggtgcgtt ggtgatctgc ggggtgactt tgaatctaaa taaaacagat gtttctacag
     2281 catctaccat gcttttgatt gcagtagggt tcctcgttct ctataaaatc tgttcaccgc
     2341 tgaataaatt cagaagtcag atcattcttt tctgtgctag cggtaacttt ttttcggtca
     2401 tctttttgca taagctgttt tcaatcaccg gtatctcagc tgtatcactt ttgctgttga
     2461 gcattctatt tttctcggca gattcgattt ttaggcattt gaccacattg gtagaatatt
     2521 tgtttgaacg taaaaatgaa aaagagcaaa agccgtttag tcgcgtagct aaaaaatttc
     2581 tatcgatatt taaacgagaa aattgacgaa aagtatctca aaaaagagta aaaaagaaca
     2641 accactcgtg tatctttcga agcaggatga gtcatccaac tttaaaacgc acgagcagtt
     2701 gctctttttt acattttcgg aatggtaacg gtgaaagtcg aacctgttcc aagatgactg
     2761 tctacagtaa tcgtaccatt aaggagttgc acgtagtctt ttacaatcgc taaccctaat
     2821 ccggtcccgc ctgaatgtcg gcttcgcgcc ttgtctacgc ggtaaaaacg ctcaaagatc
     2881 cgttcctgat cctcaatgtc gatacctata ccaaagtctt ggacagaaaa cgatagatca
     2941 tctgttgcct gatagcggat aataatctcg ctgtcgcttt ttgaatattg gacagcattt
     3001 tcaattagat ttttgataat tggataaagg atatctgttt tagtagtaaa tggttcgttc
     3061 tttccaatca atcgaatcgt caaccgtttc ttttcgatga ttggctgata attttgaacg
     3121 atttgctgga aataaggttc tgtataaatt gtctggatct cgtaagaatt ctcgccatct
     3181 tttgacagct ggatgatttc ccgaatcagt ttatctaacc ggatcgcatc tttttgcatg
     3241 atctcaagaa aagaagtcag agtttgcggg tcatcttttg caccatccag taaagtctcg
     3301 gtaaaaccaa tcagtgaagt cacaggagtt tttaattcgt gagagacgtt gccaacgaaa
     3361 tccttttgta gcttttctaa acgtcgcact ttcgttaagt catacgccac ccctagaatc
     3421 tgtccggtac cttctgaatt agtaaagtag cgcaaactca agtctaatgt ctgccgacct
     3481 tctgtaaggg tgatctcctg atggatcagc ggatgctcag gagtcacttg atgaatcagc
     3541 tgaatgagtt ttggctcttg aatgacttct gtgtaccgct gttcaggttg atagcttacc
     3601 acgtttaaat gtagctgcat ctttggatta agcagtagta aacgagaatc agcatcatca
     3661 atcaggaata cgccaatcat cagttcgttc agtaacgtat ataattgctc ttctgtggta
     3721 gtacacgccc gatacatctt actcatttgt tcgctcaacg cattaatcgt ctgatacaat
     3781 tcttcccatt gatcgggtgt ttgcatgatt acttccgcct gattaggatt ttgcaccatt
     3841 ttttttagta cgggcagcac ggttttcaat ggctggtttt tttgatagat cagataatag
     3901 atcattcctg caagaacaag gaagaaagac agaaagaaga aaaagaccca acggcgaaaa
     3961 ctctcagttc tcggtaaaaa accgcttgtc ggttctgcga tacgtaaaat ggcttctaac
     4021 tggccacttt ttttgactgg taatgcgaca tataaaagat cctcattcaa agtagtactt
     4081 ttgcgcaaag cgctacctag gttcccacca ttcaatacgg ctttgatttc gggacgggag
     4141 tttcgctgtt cattcaatgt ttcattgttt gtgtcaaata agatctttcc agtagcatcc
     4201 attaaagtaa tccgttcgtt cgactgatgg acgaattctt ccaaagcagt tttgttttgc
     4261 gctgcttgga ggttttcgac atctaattgc cggatcagca gttgtgcttt ttttgtcagg
     4321 tagctttctt gctgttccac gatctgctgt tgaaaataat gggaaatcaa ttgccagcca
     4381 ccaaaaaaca aactaactaa aagaataaga aagaagagac gttcataaat ttttttccat
     4441 ttcatcgttt gggttcctga aacttatagc caaatcctct aatagtcacc aagtactttg
     4501 ggtgttttgg atctcgctcg attttttctc gcaaatgact aacgtgtaca tctacgattc
     4561 ggctttgtcc tgcgaagtca aaattccaaa tgcggtctag caatgtgtct cggtcgatca
     4621 cacggtcttt tcgtttcatg aaataaacaa gtaattcaaa ttctttcggt gttagttcaa
     4681 tcagatggtc gtctacggtc acttgataat tggtcaaatc agcagtgatt tgtccaatcg
     4741 atagatattc aggctcagct tcatcttttg attgcttacg aggttcaatt cgtctgaata
     4801 ttgctttcat acgggctaat acttcgcgag gactaaatgg tttggtcaaa tagtcgtcag
     4861 cacctatttc taggccaatg atccgatcca cttggtcatc tttagccgtc agcatcaaaa
     4921 tcggtgtttc atttttttct tgacgcagcc gctgagtgat ctccatgcca tccatcgttg
     4981 gaagcatcac atctaaaatg atgaaatcaa atgattgttc taatgctaat tctaatcctt
     5041 cgcctccatc tgtggcactg gttaccttgt agccttcttt ttccaaattg aacgtcaaga
     5101 gcgttacaat agaaggttca tcatcaacaa ctagtatttt tttcattcaa atctccttta
     5161 aataggtttc attctgttta tattttagca tagaatcagt acaaagggct attttacaaa
     5221 agaaataaat ggtataatag gaaggcactt tgctgaatag aaaggaagag cagtcaatga
     5281 ttggaatcag tgcatgttta ggcggtctgg cctgtcgcta tgatggacag aaaaaagaag
     5341 taccagaatt gatggaacta gttgaaactg gagaagctgt catggtctgt ccagaagtga
     5401 tcggcggatt accaattcct agagatcccg ctgaaatcat tggtggaaat ggattcgatg
     5461 tgtggaaaga tcaagcaaaa gtatggacga caagcggcga agatgtaacg gaagcgtaca
     5521 aaaatggcgc gatcaaagcc tatcaaaaat tgcaggaaaa acacattgat ctagtcatca
     5581 tgaaggaaaa aagcccttca tgcggaaccc attcgattta tgacggaagt ttttccggaa
     5641 taaaaaaaaa tggtgccgga gtggctactg cttattttat tcagcaaaaa atgactgtgc
     5701 tctctgatga agaatggctg aaaactagag gtgagcagaa tggaaatcga aaagaccaat
     5761 cgaatgaacg cgctctttga attttattcg accttattga cagaaaaaca aatgaactac
     5821 atggaattat attatgcaga tgatttttca ttaggtgaga tcgcggaaga atacgaagta
     5881 agccgacaag cagtatatga caatatcaag cggacaagca agatcttgga ggattatgaa
     5941 aaaaaactcc atcttttttc taattatatt gttcgagaac aggtattgga aaatatgact
     6001 gcatatatta cagataagta cccggaagat aaaaaactac ttgaatatgt acagcaaatt
     6061 caagcaatcg aagaataaat aaaaggaatg aagaaaatat ggcatttgaa agcttaactg
     6121 aacgtctcca gcaggcgatg ggaaaaataa gaaaaaaagg aaaagtatca gaagctgacg
     6181 taaaagaaat gatgcgggaa atccgccttg ccttactaga agccgatgtt aacttgcaag
     6241 tagtcaaaga ttttactaag cgcgtacgcg aacgtgcggt aggggcagaa gtgctggaaa
     6301 gtttatcacc tgcacaacaa atcgtaaaaa tcgttgatga agaattgacg atcacacttg
     6361 gttctgaaac agcagagtta aataaatcac caaaaatacc aactgttatc atgatggctg
     6421 gtcttcaagg ggcaggtaaa acgaccttta ctggtaaatt agccaattat ttaaagaaaa
     6481 atgaaaatgc tcgtccgctc ctaatcgcag gggacgtata tcgaccagct gcgatcgacc
     6541 aattgaaagt attaggtcaa cagctagacg ttcctgtatt tgatatggga actgaagtca
     6601 gcccggtaga gatcgtccgt caagggatgg agttagcaaa agaaaagaaa aacgactatg
     6661 tattgatcga tacggcagga cgtctgcata tcgatgaaac attgatggat gagttgaaac
     6721 aaatcaaaga attgacgcaa ccaaatgaga ttttgcttgt tgtcgatgcc atgactggtc
     6781 aagatgccgt caacgtagct gatagcttca accagcaatt aggtattaca ggggtcgtca
     6841 tcactaaact tgatggggat actcgaggcg gggcagcact atcgatccgc tcagtgacag
     6901 gcgcaccaat caaattcatc ggttctggtg aaaaattgac tgacttagaa gtattccacc
     6961 cagatcgtat ggctagccgt atccttggta tgggggatat gctgacattg attgaaaaag
     7021 cgcagcaaga ctacgacgag aaaaaagcgg aagaacttgc acagaaaatg cgggaaaatt
     7081 cattcgattt caatgacttt attgaacagc ttgatcaagt gatgggtatg ggcccactgg
     7141 aagatctaat caaaatgatc ccaggaatga atcaagtccc tggaatcgaa aacgtcaagg
     7201 tagatccaaa agatgttgaa cgcaaaaaag cgatggttta ttctatgacg ccagcagaaa
     7261 gagcaaatcc tgatctgttg aacccaagcc gtcgtcgccg aattgctgcc ggttctggaa
     7321 acagtgttgt tgaagtgaat cgaatgatca aacaattcaa ggaatcaaga aaaatgatgc
     7381 agcaaatgtc caaaggggat atgaacatcc caggtatgga ccaaatgttt ggttcaggcg
     7441 tcaaaggaaa actaggcaaa atggcgatga atcgaatgat caagaaaaat aagaagaaaa
     7501 agaaaaaacg gaaataagat gaaaaaaaca gcttcggtac agactgaaaa tgtctactgc
     7561 cggagctttt ctatcagcaa tttttattct ttactaatac aattactcta aacgaagagg
     7621 tggcacagat gagatgtccg tggagtgaac aaacagaaag tatgcggcaa tatcatgata
     7681 ctgtctgggg tgttcctgaa tatgatgacc aaaagctgtt ccgaaaattg atgcttgata
     7741 ttaatcaagc cggattgagc tggcagacga ttttaaataa aagtgaagca ttcgatgaag
     7801 catatgatcg attcgatatc aaaaaagtcg caaattatgg agaaacaaaa atcagtgaac
     7861 taatggaaaa caaaggaatt attcgtaacc gaagaaaaat cgaagcagcc gtccaaaacg
     7921 ccaaagcagt cttgaaaatt caagaagaaa tcggcagttt ttcagagtat ctttggtcgt
     7981 tttctaatgg aaaaatcgtt gattcttctt atcattcaca agaagaagtc ccaacaacaa
     8041 gtcctctttc agatgaatta gcaaaagatc taaagaaaag aggatttaaa tttatcggaa
     8101 gcacaacaat ctatgctttt ttagaagctg tcgggatcat caatgatcac cttgacagtt
     8161 gtttccgcaa agaagaggtc gtacgtatcg gcagaaaggg cgaataaagc atgcgaaaaa
     8221 tcatatttta tggtgctata agtttagatg gctatttggc aacgaaaacg gatgatcttc
     8281 aatggctttt tgacactcca acaggtaagc aaactactta tgaagctttt tatgaaacaa
     8341 tcgatacgac catcatgggc agaaaaactt atcaggaagc aaaaaaatat ttggagaccg
     8401 aagcaattta tccagagaaa aagaatttcg ttttttccac ccacgcacaa ttacaattgc
     8461 cagatgctga ggtcgtaaac gaagatcctg ttgcttttct aaaaaatcta caaatgacag
     8521 aaggaaaaga tatctgggtt gtgggtggcg gtcgattgat caaaccgcta ttggaacatc
     8581 aaatgattga tgaatggtat atccagatta cccctgtatt attaggggac gggattcctt
     8641 tattccaaaa aggaacatat gaagcgcgtt ttgaaatgct agacacgaca cgattcggtg
     8701 aatttatcga attgcattat gtacgaaagt agaagtaaat aaaataaaaa aggcaactat
     8761 atgcagaaat gtcctgtaca tagttgcctt ttttattacc gttatgcttt ttactctgtt
     8821 aatttgttag aaaaatctag atacgcatct aaaatcgttt ggaagaaact taatgttcct
     8881 tcaacgatat taccattttc atcaattaga ttcgcaacgt ttccgatata tgcttccggt
     8941 tgctgcaagg ttggcacatt caaaaagaca agtgattggc gtagatggtg gtttgcgcca
     9001 aagccgccga tcccacctgg tgatgcagta acgactaaac ctggtttatg atcccaaaca
     9061 ctgtgtccat aaggtcttga gcctacatct aaagcatttt tcaatactgc tggcacagaa
     9121 cggttgtatt caggagtaac gaaaacgaca ccgtctagtc ccctcacttc ctcacgaaaa
     9181 cgagtccatt cagcaggagc ctgttctgga gtttcgagat cctcattgta aaatggcaga
     9241 tcatcgattt ttacaaatac actttcgtac ccttcaggaa gcaatttcgc aaaagcttct
     9301 gctacttttt tgttgtaaga atcttttctt aaactaccaa taaaaaatcc aatttttttt
     9361 gtcatacgat ttttcctcct tcaaactatc tataataatc ttacaaaaga taagtaactg
     9421 gagcaaataa aaacacttac tgacaaaagc tacatagaag atagattact tatttttaac
     9481 aaaaaagaag tctaaaatct tctattttct aaaaaaaatc gaatttaatc aagctgtaaa
     9541 gaaaaaaagc tttacagggt aaaaaaaaac tgttacacta catcttgtga ataaaacaat
     9601 ggaggtggaa ataaataatg gcagttaaaa tccgtttaaa acgtatgggt tctaaaaaga
     9661 gtccgtttta ccgtatcgtc gtagctgatt cacgttctcc tcgtgatgga cgtttcatcg
     9721 aaactgtagg tacttacaat cctttgaaag accctgcaga agtagtttta aaagaagact
     9781 tagttcttga ctggttatca aaaggtgcac aaccttctga cactgtacgt aacatccttt
     9841 caaaagaagg cgttatgcaa aaacaccacg aagctaaatt ctcaaagaaa taaggtgacc
     9901 ggatatgaaa gatttaagcg acttagtctt aacaatcgtt cgtccgctag tcacttatcc
     9961 tgatcaagtc aagttagacg tcgtagagtc gaaagacttt tacgagtaca atctaacagt
    10021 ggctcctgaa gatattggcc gtatcattgg aaaacaaggg cgtgttgcta aagcaatccg
    10081 cacaatcgtt tacggtgttc ggatcaatgc accgaaaaaa gtgcgtttga atatcatcga
    10141 tgataaggaa aagtaaataa acgtgctgta ctcgaatttt cgagtgcagc ttttttgttt
    10201 tttttattaa ttgaagaaat gattatttgg caaaacaagc aagaaagcgt atccttatag
    10261 gaggagggag tgaacagcca atgagaaaga aaatgttatt tattccattt gctctattgt
    10321 taagcgcttg tagttcggtt actcaagaat cagacaatac acatctatca agcagtatat
    10381 ctgaaactgt taccagtcca gaaaaaagta gtaccaaaac ggcttccacc actgaacaaa
    10441 caatggctac ctcaaataat gagaaaaaga ctgctttaga ccagttgaaa gaacaacagc
    10501 caaatgttcc catgcctcta gatgtaccag tctcttcagg ctatctcaat atcgcagcga
    10561 cacatacaaa gcaaggttat tctattttat attacagaac agatcgtccc ctcgggttga
    10621 atgctgacga attgaatcaa gaaacgccta ttgcaaccta tctatatcaa tatgggtttg
    10681 cttctagtca agaaacaatc caagttttgc agccatttga gatcgacaca aatggtcagc
    10741 aagtggactt aggttctcga atcacagggt atcagcaagg tgcagcaggt tcaagttttc
    10801 ttgaatggca agaaggaaac tggcgtattc gaatccgtgg gaataatata gaaggccaag
    10861 atcccttact attagctaaa gaaatcgtag cttatttgga agaaaacagt ctaccagcac
    10921 ctgaacaatt tggtaaaatt actgttgata tgggtgatac gacgaatcga gccgtagaag
    10981 taagctggca agaaccgaaa aatgcctata cgatcactca tcaagatcct atgtctgcgt
    11041 tgaagatggc agtttctatg aagaggttgt gaaatcagcg cgctgatctg agcccatcat
    11101 ttaactccaa gtcacaggaa gtaatcaaaa atagcacgcc gcatttttgc ataattttgg
    11161 cttaatacct taaaagaaaa ctagaattgc ctcttcacac atgctaaaat aaaacagaca
    11221 aaaaagaaat ggggatataa gttgactgaa tacttgaatg taggaaaaat cgtaaacaca
    11281 caaggtatca aaggcgaagt ccgtgttatc tcaacaacgg atttcccaga agaacgttac
    11341 aaaaaaggaa cggtgctaac actattccaa gacggaaaag cacctgtgga attaacagtc
    11401 aaaagtcatc gcaaacataa aaattttgac ttattgagtt ttgaaggaca tccttctatc
    11461 aatgatgtgg aaaaatatcg ggatggcata ttaaaagtgt caaaagacaa ctctgtagat
    11521 ctagctgaaa atgaattcta ttaccatgag atcatcggtc tgcatgtatt agaagatgga
    11581 actgagttag gtaaagtaaa agaaatcttg tcaccaggtg ccaatgatgt ttgggtcgtt
    11641 caacgtccga aaaaagctga tatattgctt ccatatatcg cttctgtcgt caaagaagtc
    11701 gatttagaag ctggagtggt ccgtgtagaa attccagaag gactgattga cgatgaagat
    11761 tgatgtattg actttatttc caagaatgtt tgaaggtcct atgggggaat ccatcatcgg
    11821 taaggctgta gataaaggat tattagatat caatatctct aacttccgcg attattcaga
    11881 taacaaacat cagacagtag atgattatcc atacggcggt ggagcaggta tgctgctcaa
    11941 agtccagccc atctatgata acatccaagc cattgaaaaa gctgcaccgg atgtcaaaaa
    12001 aagagtaatt cttcttgacc cagctggtaa acgattcgat cagaaaatgg cagaagaatt
    12061 ttcacaagaa gaacacttag tattcatttg tggtcattat gagggatatg atgaacgtat
    12121 ccgcacatta gtgacagatg aagtttcctt aggagattat gtccttactg ggggagaatt
    12181 aggtgcgatg gtcatgatcg atgcaacagt tcggcttttg ccagatgtgt tagggaatca
    12241 gacttcagct caaacggatt cttattcaac cggtttattg gaacatcccc aatatactcg
    12301 cccggcagaa tataaaggga tgaaagtacc agaagtattg acaaatggaa atcataaatt
    12361 gattgaagaa tggcaattaa aagaatcact aagaaggacc tatcaacgtc ggcctgatct
    12421 tttggaaaca cttgaatgga cacctcaaat gacgaaattt ttagaagaaa tcaagaaaga
    12481 agaacaagaa aaaacagcag atgaagccaa gtgacttttt ctaaaatctt ctttacagta
    12541 ggttgataat atgttagtat tttatggtga gtgcaaaagc acttaactat tacgatattc
    12601 cgctgtgaga aaagctcata agaatatttg gaataaggag aagaaaacca tgaatccatt
    12661 aatcgaagaa ttaacaaaag aacaattacg ttctgacatc ccagcgttcc gccctggtga
    12721 cactgtgcgt gttcacgcaa aagttgttga aggtactcgt gaacgtatcc agttatttga
    12781 aggtgttgtt atcaaacgcc gcggtgctgg aatcagcgaa acttatacag tacgtaaagt
    12841 ttctaacgga gtgggcgttg aacgtacatt cccattgcat acaccacgtg ttgcaaaaat
    12901 cgaagtagtt cgctacggta aagtacgtcg tgcaaaattg tactac
//
LOCUS       DACEEZ010000081        12928 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20313_2017
            SAMN12639966-rid9879223.denovo.081, whole genome shotgun sequence.
ACCESSION   DACEEZ010000081 DACEEZ010000000
VERSION     DACEEZ010000081.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639966
            Sequence Read Archive: SRR10023158
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12928)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12928)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741683.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741683.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:44:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,016
            CDSs (total)                      :: 2,962
            Genes (coding)                    :: 2,889
            CDSs (with protein)               :: 2,889
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 36 of 73
            Pseudo Genes (incomplete)         :: 29 of 73
            Pseudo Genes (internal stop)      :: 18 of 73
            Pseudo Genes (multiple problems)  :: 8 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12928
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639966-rid9879223.denovo.081"
                     /isolate="E20313_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            <1..1436
                     /locus_tag="IWO89_002274"
     CDS             <1..1436
                     /locus_tag="IWO89_002274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HAQ0456992.1"
                     /translation="QQLLAFCGMVALILLIVGVSFTQLTKQTIEENNYQQLFGYAESV
                     EKTTQTYADTLPQFSQDQAFQNALLLTEQVLTEQNVNFIFIDKNERVIYPTNTALLNF
                     TLTADQWKNLRNGRQEKFTSNKNILGQAQATSYALVPFNLNHEFYGALVVSQPARNID
                     NSVRPITLNLFKGFIFSSIVAVIASYAFAAFQVKRINRLRNATKEVTNGNFDVQLPVH
                     DKDEFDELADDFNKMTNSLKESQAEIEEQENRRRQFMADASHEMRTPLTTINGLLEGL
                     EYNAIPENQRENAIKLMKNETERLIRLVNENLDYEKIRTNQISMVIKKFNGTETLKNI
                     VAQLEAKAEAAGDTLTLKAVDDIDVYADYDRFVQIMVNIIQNAIQFTENGQITITLEK
                     GYLETIITIEDTGIGMSEQQMKSIWDRYYKVDPSRKNTKYGESGLGLPIVQQLVRLHK
                     GKLEVESELGKGTKFAVRLPDVEIKKE"
     gene            complement(1506..2015)
                     /locus_tag="IWO89_002275"
     CDS             complement(1506..2015)
                     /locus_tag="IWO89_002275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0456993.1"
                     /translation="MKPEYLIHAEHLTVILCPLEKQHKILEHLKKEVLVAKLDNFAII
                     QKNWPMFPYLNLKDHVLLDVPEKEIKQDRLAYQEKLDISPSLLNCAAAELTSFEKVKL
                     QLLHALLSKRNNIVIEDTFDELSVLEIQELLHLLSYLAHEENQGILLFTHDATIAQSP
                     YIDRLEPAG"
     gene            complement(2005..2730)
                     /locus_tag="IWO89_002276"
     CDS             complement(2005..2730)
                     /locus_tag="IWO89_002276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0456994.1"
                     /translation="MKIIRYATARLIYYKKQTILYCLFSSFSAFLLIACLTLYHLQSA
                     LANQVQDRLSFIGASASTELLPALELASRFYIKGIFSLFFLFAFLFIFFFYYSVRQNK
                     QELINWRLTGLSKTKLFLFIFWQLFFPLFLCCILVLLWIVSFQSVYEALLQRINFLVL
                     KTTELPDIRDILTSVSGLTIPMDQQTFFTIDFQNDLFFTDILKGFAQTLIGLSGCCLI
                     LSLFPFTFFIHHLQSRRPGRNET"
     gene            complement(2953..5073)
                     /locus_tag="IWO89_002277"
     CDS             complement(2953..5073)
                     /locus_tag="IWO89_002277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HAQ0456995.1"
                     /translation="MKCIKTPAFLNKRLGFFSLLVVLLWLKTIFAYLVDFHLGIESGI
                     QYFILFINPIATTLLLLGIALYIRRPRVAYITMMVIYFLLTLLVFSNVTYYREFTDFI
                     TINTILGAGKVASGLGESAIRLFRPYDVIYWIDFIILTVLLIAKKIKLDPRPIRARMA
                     FAISTLAVMIFSGNLFMAEADRPELLTRTFSRDYLVKYLGLNAFMVYDGVQTYQTNQV
                     RAEASPNDMKEVENYVKEHYAAPNDDLFGLAKGKNVIYIHLESFQQFLIDYKLTDENG
                     LQHEVTPFINSLYHSNSTFSFDNFFHQVKAGKTSDAETLMENSLFGLNQGSLFTQLGG
                     KNTFQAAPDILSQTQGYTSAVFHGNAGTFWNRNETYKNLGYDYFFDSSYYDVNDDNSF
                     QYGLHDKPFFQQSVQYLERLQQPFYSKFIAVSNHYPYSKFTNDEAGFPLANTNDETIN
                     GYFATANYLDTAVQEFFNYLKESGLYDNSVIVLYGDHYGVSNSRNKNLAELVGKTSST
                     WTNYDNAQMQRVPYMIHIPGQTQGGINHTYGGQVDALPTLLHLLGVDTKNYIQLGQDL
                     FSKDHEQLVSFRDGDFVTPKYTYYGGTIYSNETGEAITEPTEEVQNEIDSLKEKVDKQ
                     LAVSDQINNGDLLRFYTNSGLDPIDTSDYDYKNGLEKLKEAEKSLGDKSTSVFSEHNG
                     QSTVDLYKTQTYQELHGTTTSSSE"
     gene            5303..6481
                     /locus_tag="IWO89_002278"
     CDS             5303..6481
                     /locus_tag="IWO89_002278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent rRNA methyltransferase"
                     /protein_id="HAQ0456996.1"
                     /translation="MKVKVKKRASNRLKKGYPLIQKEDLAHPQKETTQWVELVDSQNQ
                     FIAKGYLGKQNKGIGWVLSQQNIPFDQSFFEEFFVKSKEKRTFFFSDDSTTAFRIFNG
                     EGDGIGGLIVDYYAGYAVFSWYNETLYAHRELLIQAFREIYPEIVGGYEKIRFESVNL
                     PESQFIYGKEAPEPLIVKENGVKFAVYLNEGLMTGIFLDQKEVRGSLAEGFASGKTVL
                     NMFSYTGAFSVAAAMGGAIETTSVDLAKRSLKKTQEQFEVNGLDVDQQKIIVMDVFEY
                     FKYAKRKQFLYDMIVLDPPSFARNKKKVFRVAKDYGELVKDSLDILAEDGILIASTNA
                     ANVSIEQFQTMIEEEFHAKNVSFEQLALYRLPKDFQTTDTFLEGNYLKVFVYQIKNNH
                     "
     gene            6497..7255
                     /gene="aroD"
                     /locus_tag="IWO89_002279"
     CDS             6497..7255
                     /gene="aroD"
                     /locus_tag="IWO89_002279"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I 3-dehydroquinate dehydratase"
                     /protein_id="HAQ0456997.1"
                     /translation="MVWVKGLNIGSGRPKICAPLIGAVREEVLREAVLAREAGADLVE
                     WRVDHYREVFSFDEVAETLVLIHEMLDGLPLLFTFRTLAEGGEQDISIRQYRDLYEHV
                     IHTGLVDMVDIELFKVESLGKRLIEEIKSLNIPLIISSHDFKETPADPVLLYRLNVME
                     HFGADIGKLAVTPNNERDVLRLMELTRRANAFVSMPIITMSMGNLGKISRLAGGTTGS
                     VMTFGSLSSDSTSAPGQLPVKALKQIIQLLNEPL"
     gene            7278..7763
                     /gene="ybaK"
                     /locus_tag="IWO89_002280"
     CDS             7278..7763
                     /gene="ybaK"
                     /locus_tag="IWO89_002280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cys-tRNA(Pro) deacylase"
                     /protein_id="HAQ0456998.1"
                     /translation="MAKKKIVKTNAIRMVEQKKIPYTEHEYEWDESHLSASSVAEQLP
                     ESQSRIFKTLVAVGNVTGPLVAVIPGEAELNLKKLAKVSGNKKVEMLHLKDLEATTGY
                     IRGGCSPIGMKKLFPTYLDQIAESYEQIIVSAGRRGLQMELAPQDICALTSGQFADIK
                     Q"
     gene            complement(7834..9087)
                     /locus_tag="IWO89_002281"
     CDS             complement(7834..9087)
                     /locus_tag="IWO89_002281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HAQ0456999.1"
                     /translation="MEKLYDVIVVGAGTSGMMAAISAAEQGARVLLIEKNKKAGKKLL
                     MTGGGRCNVTNNRPVDDLIAHIPGNGKFLYSTFAQWNNFDIMNFFESQGVHLKEEDHG
                     RMFPVTNKSKTIIEALLNRLKELDVTLLFSTRVEKLIHKEHKIYGIRTEFEEFHAPAI
                     ILTTGGRTYPSTGSTGDGYKIVKRVGHTVTPLYATESPLISDEPYIQEKTLQGLSLQD
                     ITLRVLNKKGRVLTEHTMDLLFTHFGISGPAALRCSSFVNKELEKTGEPVTLSLDCFP
                     TQTKQELIHILTEKSKATKKNLVNAWHGILPERLLVFFLERLEMDHLTGQQASQKQIQ
                     DFVQLCKEFKLSINKTFPIEKSFVTGGGVSLKEIYPKTLESKIIDGLYFAGELLDVNG
                     YTGGFNITAAFATGHVAGMNAGQRA"
     gene            9205..10554
                     /gene="gdhA"
                     /locus_tag="IWO89_002282"
     CDS             9205..10554
                     /gene="gdhA"
                     /locus_tag="IWO89_002282"
                     /EC_number="1.4.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-specific glutamate dehydrogenase"
                     /protein_id="HAQ0457000.1"
                     /translation="MTNATEYVQAIQEKLHQKDQGQEEFLQAIDEFMPTVMSFLDTHP
                     EYIEKNILELLTEPERVIQFRVPWQDDEGNWRVNRGYRIQYNSAIGPYKGGLRFHPSV
                     NLSILKFLAFEQIFKNSLTGLPIGGGKGGSDFDPKGKSDNEIMRFCQSFMLELSKHIG
                     PSIDVPAGDIGVGAREIGYLFGEYKRLKKYDTGVLTGKPLDFWGSKIRTEATGYGLVY
                     YVKHLLNEEKDSFNQKTVFVSGSGNVAIYAIEKVQELGGKVVTCSDSSGYIYDPEGID
                     VKLLKEVKEINRQRLTEYANQRPSAIYHAGESVWTLKEKADIALPCATQNEIDESLAE
                     ILVENGIKIVAEGANMPCTVKAVSVLHEAGVWYCPGKAANAGGVAVSALEMSQNVQRL
                     TWEREQVDEQLDQIMETIYHTCRQTAAEYGDEKNLLLGANVAGFEKVAKAMAAEGLV"
     gene            10666..12012
                     /locus_tag="IWO89_002283"
     CDS             10666..12012
                     /locus_tag="IWO89_002283"
                     /EC_number="5.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HAQ0457001.1"
                     /translation="MAHIHFDYSKVAPFVNEQELGYMQSQVTAAHNELREGTGAGSDF
                     RGWIDLPTNYDKEEFARIKEAAKKIQSDSEVLVVIGIGGSYLGARAAIDFLNHTFFNL
                     LDSNTRKAPQIFFAGNSISSTYIADLIEVIGDRDFSVNVISKSGTTTEPAIAFRVFKE
                     LLVKKYGQEEANKRIYATTDKAKGAVKVEADAEGWETFVIPDDIGGRFSVLTAVGLLP
                     IAVSGADIDALMQGAADASKAYSSDKLEENEAYQYAAMRNILYRKGKVTELLINYEPG
                     MQYFSEWWKQLYGESEGKDQKGIYPSSANFSTDLHSLGQFIQEGRRNIFETVVKVEKP
                     RKSITIPEQAEDLDGLGYLQGKEVDFVNTKAFEGTLLAHTDGDVPNLLVKIPEMDAYT
                     LGYTMYFFEIAVGISGYLNGINPFDQPGVEAYKKNMFALLGKPGFEDLAKELNERL"
     gene            complement(12320..12706)
                     /locus_tag="IWO89_002284"
     CDS             complement(12320..12706)
                     /locus_tag="IWO89_002284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YxeA family protein"
                     /protein_id="HAQ0457002.1"
                     /translation="MKKILGLIVIILIAFIGWKGWDYYQSTYVGKDYYAVVKAPMPAE
                     TDIKADNGEVIGRGFKYNVDAYAENGEKRQLDFDVITSGDSANGSAYPEGTILQLKAS
                     EKRIIEKKVITSDKVPSSVKEKLGLS"
     gene            complement(12755..>12928)
                     /locus_tag="IWO89_002285"
     CDS             complement(12755..>12928)
                     /locus_tag="IWO89_002285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS982 family transposase"
                     /protein_id="HAQ0457003.1"
                     /translation="TVETVFSSLEKLGCQNFNSRSVKGLESRFESILLAYSVLLSRAQ
                     RRFEGTLRYSLGY"
BASE COUNT         4269 a         2305 c         2613 g         3741 t
ORIGIN      
        1 atcagcaact cttagctttt tgcggaatgg tcgcgttgat cttattgatc gttggagttt
       61 cttttacaca actgacaaaa caaacgattg aagaaaacaa ttaccagcaa ctgtttggat
      121 atgctgaatc agttgaaaag acaacgcaaa cctatgccga tacgctcccg cagtttagtc
      181 aagatcaggc gtttcaaaat gcattgctgc tgacagagca agtactgaca gaacaaaatg
      241 tgaactttat atttatcgat aaaaacgaac gtgtcattta tccgactaat acagctttac
      301 tgaattttac cttaacagct gatcaatgga aaaatctgag gaatggacgt caagaaaaat
      361 tcacatcgaa taaaaatatt ttaggacaag cacaggcgac ctcttacgca cttgtgccat
      421 ttaatttgaa tcatgaattc tatggagcac ttgtagttag tcaacctgcg cgaaatatcg
      481 ataacagcgt tcgtccaatt acactgaacc tgttcaaagg atttattttt tccagtatcg
      541 ttgcagttat tgccagctat gcttttgcag cattccaagt taagcgtatc aatcggttaa
      601 ggaatgcgac gaaagaagtg acgaatggta attttgatgt acagctcccc gtacatgaca
      661 aagatgaatt tgatgagtta gcagatgatt tcaataaaat gacgaattca ttgaaagaat
      721 ctcaagcaga aattgaagaa caagaaaatc gtcgaagaca atttatggca gatgcttctc
      781 acgagatgag aacacctctt acgacaatca acggacttct ggaagggtta gaatacaatg
      841 caatccctga aaatcagcga gaaaacgcga ttaaattgat gaaaaatgaa acagagcgtc
      901 tgatccgttt ggtgaatgaa aacttggatt atgaaaaaat ccgtacgaat caaatcagta
      961 tggtcattaa aaaattcaat ggaacagaaa cattgaaaaa tattgttgca cagttagaag
     1021 ctaaagcaga agcagcagga gatacattga cattgaaagc cgttgatgat attgatgttt
     1081 acgctgatta tgaccgattt gtccaaatca tggtcaatat tattcaaaac gcgatacagt
     1141 ttactgaaaa cggccaaatc acgattactt tagaaaaagg ctatcttgaa acgatcatca
     1201 cgattgaaga tacgggaatt ggaatgtctg aacaacaaat gaaaagtatc tgggatcgtt
     1261 attataaagt agatccttca agaaaaaata caaaatatgg tgaatcagga ttaggtttac
     1321 caatcgtgca gcaacttgta cgcttgcata aaggaaaatt agaagtagaa agcgaactag
     1381 gaaaaggaac aaaattcgct gttcgtttgc ctgacgtaga aatcaaaaaa gagtaaaaaa
     1441 caagcggtat aaaagatcat gaatcgatct tctataccgc ttgtttttac tataaaatga
     1501 ttgatttagc ccgcgggctc taaacgatca atataaggac tttgagcaat agtagcatca
     1561 tgagtaaaga gtaagatacc ttgattttct tcatgagcta aataagaaag caagtggagt
     1621 agctcttgga tctccaatac actaagttca tcaaatgtat cttcaataac aatattgttt
     1681 cgcttggaaa gcaaggcatg aagtaattgc agctttactt tttcaaaaga cgtcaactca
     1741 gctgccgcac aattaagcaa ggaaggagag atatcaagtt tttcttgata ggccaaacga
     1801 tcttgtttga tttctttttc cggtacatcc aacaggacat ggtctttaag attcagataa
     1861 ggaaacatcg gccaattttt ttggatgatc gcaaaattat ccagttttgc aacaagaacc
     1921 tcttttttca agtgctccaa tattttatgc tgtttttcga gaggacacaa aataacggtc
     1981 aagtgttcag catgaatcaa atattcaggt ttcattacgt ccaggcctcc tactttgcaa
     2041 atgatggata aaaaaagtga aagggaatag actaagaatg agacagcatc cactgagccc
     2101 aataagagtt tgggcaaatc ccttaagtat atctgtgaaa aaaagatcat tttggaaatc
     2161 gatggtaaaa aaagtctgtt gatccatcgg aattgtaagt ccgcttaccg aagtcaagat
     2221 atcacggata tctggcagtt cagtagtctt taagacaaga aaattgatcc tttgaagaag
     2281 cgcttcataa actgactgga agcttacgat ccacaataaa accaagatac aacaaagaaa
     2341 gagaggaaag aacagctgcc agaaaataaa aaggaataat ttggttttag aaagaccagt
     2401 caggcgccaa ttgatcaatt cctgcttgtt ttgcctgact gaatagtaaa agaaaaagat
     2461 aaaaaggaag gcaaatagaa aaaataaact gaatatgcct tttatataaa atcggctagc
     2521 taattctaaa gccggaagaa gctctgtaga tgcagaagcg ccaataaatg aaagccgatc
     2581 ttggacttga ttggctaaag ccgattgcaa atggtaaaga gtcaggcatg ctatcaataa
     2641 aaaagcagaa aagcttgaaa ataaacaata gagtattgtc tgctttttgt aataaattaa
     2701 tcgagcagta gcatagcgga ttattttcat acttttcacc tctataaaaa gtatagaaaa
     2761 tgaatatgaa taaagtatga aattccttta gtgaaatcac gaattaaaac cagaaaatat
     2821 tttgtaaaaa gcaagaggtt gtgaagaagc tgccctattc tattaccgta gatgcaagct
     2881 tttgaacact gtttattcga aaagaggagc agcgacaata attttgtcgc tgctcctttt
     2941 aaatttattt tgtcattcgg aagaacttgt ggtcgttccg tgaagttctt ggtacgtttg
     3001 cgttttataa agatcaacag ttgattgtcc gttgtgttca ctaaagacac tagttgactt
     3061 gtcaccaaga gatttttcag cttcttttaa tttctctaag ccattcttgt agtcataatc
     3121 actggtatca attgggtcta atccactatt cgtatagaac cgaagaagat caccattatt
     3181 gatttggtca gaaacagcta actgtttatc aactttttct ttcagtgaat caatttcatt
     3241 ctgtacttcc tcagtaggtt cagtaatagc ttcaccagtt tcgttactat agatcgtacc
     3301 accgtagtaa gtatattttg gtgtaacaaa atcaccatca cggaaagaaa caagctgttc
     3361 atgatctttc gagaatagat cttgtcctaa ctgaatataa ttttttgtat ctacacctag
     3421 taagtgaagt aatgtaggca aggcatcaac ttgtccgccg taagtgtgat tgattccgcc
     3481 ctgtgtttgt ccagggatat ggatcatata agggacacgc tgcatttgtg cattgtcgta
     3541 gtttgtccaa gtagaacttg tttttccaac taattcagct agatttttat ttcttgaatt
     3601 cgagacaccg taatggtcac cgtaaaggac gatgactgaa ttatcataca agccgctttc
     3661 tttcaaataa ttgaagaatt cttgtacggc ggtatctaaa taattggctg tcgcaaagta
     3721 accgttgatc gtttcatcat ttgtattggc taacgggaat cctgcttcgt cattggtgaa
     3781 ttttgaataa ggataatggt tagatacagc aatgaatttt gaataaaatg gttgttgcag
     3841 acgttccaag tattgtacgg attgttggaa aaatggttta tcatgcaacc cgtattggaa
     3901 cgaattgtca tcattaacat cataataaga agaatcaaag aaataatcat atcctaaatt
     3961 cttgtatgtc tcgtttcgat tccagaatgt accagcgttc ccatggaata ccgcactagt
     4021 gtaaccttgt gtttgactta aaatatcagg tgcagcttgg aaagtatttt ttccgcctaa
     4081 ttgtgtgaac aaggagcctt gattcaagcc aaacaatgaa ttttccatca gcgtttccgc
     4141 atcgcttgtt tttcctgctt ttacttgatg gaaaaagttg tcaaaactga acgtgctgtt
     4201 gctgtggtaa agactattga tgaaaggtgt aacttcatgc tggagaccat tctcatctgt
     4261 caatttataa tcgatcagga attgctggaa gctttctaaa tggatataaa tcacattttt
     4321 tcctttagcg agcccgaaca aatcgtcatt tggtgctgca taatgttctt ttacataatt
     4381 ttctacttct ttcatatcat tgggacttgc ttctgcccgc acttggtttg tctggtaagt
     4441 ttgaacaccg tcataaacca tgaaagcatt caagccaaga tatttgacca aataatcacg
     4501 agagaatgtt cgagtcagca attctggacg atcagcttcc gccataaaaa gatttcctga
     4561 gaaaatcata actgctaatg tagagatcgc aaaagccatt cttgctcgga tcgggcgagg
     4621 gtccaacttg attttttttg caatcagcag aacagtcaag ataataaaat cgatccaata
     4681 gatcacatca tatgggcgaa acagtcgaat ggcactttct cccagaccac tcgctacttt
     4741 cccagcacct aaaatcgtgt tgatcgtaat gaagtccgtg aattctcgat aataagtgac
     4801 attcgaaaaa acaagcaatg tcaaaagaaa ataaatgacc atcatcgtga tataagcaac
     4861 tctagggcgt ctgatataaa gagcaattcc aagtaataac aatgtagttg caatagggtt
     4921 gataaacaag ataaagtatt gaatgccact ttcgattccg agatggaaat caactaaata
     4981 ggcaaaaatc gttttcaacc aaagtaaaac gactagtaaa gaaaaaaatc cgagcctctt
     5041 attcaaaaag gcaggtgttt ttatacattt caagataatc ttcctttctg cacttcttaa
     5101 tcacagaagt gtcatgaaac catgttacac agctcactta ttctcattta ttttactagt
     5161 atgacaatat agtgtcaatt gaaaccagaa gtttatgctg tttcattaag gttttcttta
     5221 agcttcatga aaaaatgagc agtaagacaa gtcctatgtt ttttgttata cttgggaaga
     5281 gaaagctaaa gcaggtgaag gaatgaaagt aaaagtaaaa aaaagagcaa gcaatcgatt
     5341 gaaaaaagga tatccactga tccaaaaaga agaccttgct catccccaaa aagaaaccac
     5401 gcaatgggtg gaattagttg attcgcaaaa ccaatttatc gcaaaaggat atctaggaaa
     5461 gcagaataag ggaatcggtt gggtattgag ccagcagaac atcccatttg atcaatcttt
     5521 ttttgaagaa ttttttgtga agtcaaaaga aaaaagaact ttcttttttt cagatgattc
     5581 tactaccgct tttcgtatat tcaatggaga aggagacgga atcggcggtc tgattgttga
     5641 ttattatgca ggttatgctg ttttttcttg gtataacgaa acgttatatg cacatcgtga
     5701 attattgatt caagcttttc gtgaaatcta cccggaaatc gtaggaggct atgagaaaat
     5761 tcgatttgaa tcagtaaatc tgcctgagag ccagttcatt tacggaaaag aggcaccgga
     5821 acccttgatc gtaaaagaaa acggagtaaa atttgccgtt tatttaaatg aaggattaat
     5881 gactgggatc tttttagacc aaaaagaagt acggggatca ttagcagaag gtttcgcttc
     5941 tgggaaaaca gttttgaaca tgtttagtta taccggcgcc ttttctgttg ctgccgcaat
     6001 gggaggggca atcgaaacaa caagtgtcga tctagcaaaa cgcagtttaa aaaaaacaca
     6061 ggaacagttt gaagtgaatg gtttggatgt cgatcagcaa aaaatcattg ttatggacgt
     6121 attcgagtat ttcaaatacg ccaaaagaaa acaattttta tatgacatga ttgtattgga
     6181 tccaccaagt tttgccagaa ataagaaaaa agtctttcgc gtagcaaaag attatgggga
     6241 actggtcaag gatagtctgg atattttagc agaagacggg atacttattg cgtctacaaa
     6301 tgctgcgaat gtctcgatag aacaattcca aacaatgatt gaagaggaat tccatgccaa
     6361 aaatgtttct tttgaacaac ttgctttata tcgtctgcca aaagatttcc agacgacgga
     6421 taccttttta gaaggcaatt atctgaaagt gtttgtttat caaattaaaa ataaccatta
     6481 gaaggagcga tgcagaatgg tttgggtaaa gggattgaat ataggaagcg gtagaccaaa
     6541 gatttgtgcg cctcttatcg gggccgtccg agaggaagta ttacgtgaag cagttcttgc
     6601 acgtgaagca ggggccgatt tagtcgaatg gcgagtcgat cattatcgtg aagttttttc
     6661 ttttgatgaa gtagcagaaa cattggttct tatccatgag atgcttgatg gactgccgtt
     6721 attatttacg tttcgtacct tagctgaagg cggagaacaa gacatatcga tccgtcagta
     6781 tcgcgacctt tatgaacatg tgatacacac aggattagta gatatggtcg atatcgagtt
     6841 gttcaaagta gaaagtctag gaaaaagact gatagaggaa atcaagtctt taaacattcc
     6901 tttaattatc agtagtcatg attttaaaga aacaccagca gaccctgtct tgctttaccg
     6961 attgaatgtt atggaacatt ttggagccga tatcggaaaa ctggcagtaa ctcccaataa
     7021 tgaacgagat gttttgcggt tgatggagtt gactagacgg gcaaatgctt ttgtctccat
     7081 gcctattatt acgatgtcca tgggaaatct tggtaaaatt tctcgtctgg ctggcggaac
     7141 gacgggttcc gttatgacat ttggttcctt aagcagcgat tctacttctg ctccaggaca
     7201 attaccggtc aaagcattga agcaaattat tcaattatta aatgaaccgt tgtagaacaa
     7261 gtttgaggtg atattttttg gcaaagaaaa aaatagtaaa aacgaatgca atccgtatgg
     7321 tcgaacaaaa aaagattccc tatacagagc acgaatatga gtgggacgaa agccacctaa
     7381 gtgcgtcatc ggtcgctgaa cagcttccag aaagccaatc tcgtatattt aaaacactgg
     7441 ttgctgtggg caatgtgacg gggcctcttg ttgctgtgat accgggagaa gccgaattga
     7501 atctaaaaaa attagcaaaa gtttcaggga ataaaaaagt agaaatgctc catttaaaag
     7561 atttggaagc aacgaccgga tacatacgtg gtggctgctc gcctataggt atgaaaaaac
     7621 tatttcctac ttatttagat caaattgcag aaagctatga acaaatcatt gtctcggctg
     7681 gcagaagagg gctacagatg gagttagcac ctcaagatat ctgtgcatta acgagcggac
     7741 aatttgcaga tataaaacaa taaatcatac gtataaaaga aagaaggagg ccgggatgtt
     7801 gtttgtccag tctccttctt tcttttttat acattatgct ctttgcccgg cattcattcc
     7861 tgctacatgc cctgtagcga aagccgctgt gatattaaag ccgccagtat aaccattgac
     7921 atccagtaat tctcccgcaa aatacagacc atcaatgatt ttactctcca atgttttagg
     7981 ataaatttcc tttagagaaa cccctccgcc agttacaaat gatttctcaa taggaaaagt
     8041 tttgttgatt gatagcttaa actctttgca tagttgaaca aaatcttgaa tttgtttttg
     8101 agaggcttgt tgtcctgtca gatgatccat ttcaagccgc tcaagaaaga aaactaacaa
     8161 tcgctcaggc aatataccat gccaagcatt gaccaagttc tttttggttg ctttactttt
     8221 ttctgtcaat atatgaataa gctcttgttt ggtttgtgta gggaaacaat ccagagaaag
     8281 tgtcactggt tctcccgttt tctctaattc tttattgaca aaacttgaac aacgcagtgc
     8341 agccggacca gaaataccga aatgggtgaa aagtaaatcc atcgtatgtt cagtcaatac
     8401 acgacctttt ttatttaaaa cgcgtaaagt gatgtcttgt aaagaaagcc cttgaagtgt
     8461 tttctcctga atatacggtt catccgatat caagggggac tctgtcgcat aaagaggcgt
     8521 caccgtgtga cccactcgct tcacgatctt gtacccatct ccagtagaac cagtagatgg
     8581 ataagtccgt ccaccagttg ttaagataat ggcaggagca tggaactctt cgaattccgt
     8641 gcgtataccg taaattttat gttctttgtg gatcaatttc tctactcgag tagagaatag
     8701 aagcgtcaca tctagttctt tcagacgatt gagcaatgct tcgataatcg tttttgattt
     8761 atttgttaca ggaaacatcc gtccatgatc ctcttctttt aaatgtacgc cttgtgattc
     8821 aaagaagttc atgatatcga aattattcca ttgcgcaaac gtactatata aaaactttcc
     8881 atttccagga atatgtgcaa tcaaatcatc aacaggacga ttgttcgtaa cattgcaccg
     8941 accaccacct gtcatcagta gtttttttcc tgcttttttg tttttttcga tcaagagtac
     9001 gcgtgcgcct tgttcagctg cactgatcgc tgccatcatc ccgcttgttc cagcgccgac
     9061 tacgatcaca tcatacagtt tttccattcc ctcacctcaa ggaaagtgta gcatatctga
     9121 agaaagaaaa cagaaaaaaa atgttttgag ccaaaagaga aaatgagcta aaatgaggga
     9181 agataagcaa aggagcgtgg aacgatgaca aacgcaacag aatatgttca agcaattcaa
     9241 gaaaagctac atcaaaagga tcaagggcaa gaagaatttt tacaagcaat cgatgaattc
     9301 atgcccacgg ttatgtcttt tttggacact catcccgaat atatcgaaaa gaatatctta
     9361 gaacttttaa cggaaccaga aagagttatt caatttcgag tgccttggca agatgatgaa
     9421 ggaaactggc gagtaaatag agggtaccgt atccaataca actcagctat cggcccttat
     9481 aaaggtggat tgcgcttcca tcctagtgtc aatctaagta tcttgaaatt tttggctttt
     9541 gaacagattt tcaagaatag tttaaccggt ctgccaatcg gtggaggaaa aggcggaagt
     9601 gacttcgatc ctaaaggaaa atctgataat gagatcatgc gtttttgtca aagtttcatg
     9661 ctggaattgt caaaacatat cggcccatcg attgatgttc ctgcgggaga tatcggtgta
     9721 ggagctagag aaatcggcta tttgttcggc gaatataaac gattgaaaaa atatgatact
     9781 ggtgtgttga caggtaaacc tttggatttt tggggaagta agatacgtac ggaagctact
     9841 ggttatggat tagtgtatta tgtcaaacat ctattgaacg aagaaaaaga ttcatttaat
     9901 caaaaaacag tttttgtttc cggaagcggg aatgtagcca tttatgcaat cgaaaaagtg
     9961 caagagctag gcgggaaagt tgttacatgt tcagattcta gcgggtacat ctatgatcca
    10021 gaaggaatag atgtgaaact attgaaagaa gtcaaagaaa tcaatagaca acgtttgacg
    10081 gaatacgcaa accaacgtcc ttctgctatt tatcatgctg gagaatctgt ttggacattg
    10141 aaagaaaaag cggatatcgc tttgccatgt gctactcaaa atgaaataga cgaaagcttg
    10201 gccgaaatct tagtagaaaa tgggatcaaa atcgtagcag aaggcgctaa tatgccatgt
    10261 acagtaaaag ctgtttctgt tcttcatgaa gcaggcgtgt ggtattgtcc aggaaaagct
    10321 gctaatgctg gaggagtagc agtatctgct ttagaaatga gtcagaacgt ccagcgattg
    10381 acttgggaaa gagagcaagt ggatgagcag ctggatcaaa tcatggaaac gatttaccat
    10441 acgtgtcgcc aaacagcagc cgagtacggc gacgaaaaaa atctgcttct tggagcaaat
    10501 gttgctggat ttgaaaaagt tgccaaagca atggcagcag aaggtttagt ttgaaaaatg
    10561 aaactttctt ggaaatattg tcagcatttt gtttatttgc ttgaaaaata ataaaaaaaa
    10621 acgtaaagta ttacctgtag agtaaaccta taaggaggag aacaaatggc acacattcat
    10681 tttgactatt caaaagtagc accgtttgtc aacgaacaag agttagggta catgcaaagc
    10741 caagtaacag cagcccacaa cgaattacgg gaaggtactg gcgcaggaag cgacttccgc
    10801 ggctggatcg atttaccaac gaactatgac aaagaagaat ttgcacgtat caaagaagca
    10861 gctaaaaaaa tccaatctga ttctgaagta ttagttgtta tcggtatcgg tggttcttat
    10921 ttaggcgctc gtgcagcgat cgacttcttg aatcacacat tcttcaattt attagatagc
    10981 aacacaagaa aagcaccaca aatcttcttt gccggaaatt caatcagctc aacatatatt
    11041 gctgacttga tcgaagtaat cggtgatcgc gacttttcag taaacgttat ttcaaaatct
    11101 ggaacaacta ctgaaccagc gattgcgttc cgtgtattca aagagcttct tgttaagaaa
    11161 tatggacaag aagaagcaaa caaacgtatc tacgctacaa cagataaagc aaaaggtgca
    11221 gttaaagtag aagcagatgc agaaggttgg gaaactttcg tgattcctga tgacatcggc
    11281 ggacgtttct ctgtattaac tgctgttggt ttgttaccaa tcgctgttag tggtgcagat
    11341 atcgatgcat tgatgcaagg tgccgctgat gcaagcaaag catactcaag cgacaaacta
    11401 gaagaaaacg aagcttacca atacgcagca atgcgcaaca ttctttaccg taaaggcaaa
    11461 gtaactgagt tgttgatcaa ctatgaacca ggaatgcaat acttctcaga atggtggaaa
    11521 caattgtatg gtgaatctga aggaaaagac caaaaaggca tctatccttc aagcgcaaac
    11581 ttctcaactg atttgcactc tttaggacaa ttcatccaag aaggacgtcg caatattttt
    11641 gaaacagttg tgaaggttga aaaacctcgt aaatcaatca caattcctga acaagcagaa
    11701 gatttagacg gtctaggcta cttgcaagga aaagaagtgg acttcgtaaa tacaaaagca
    11761 tttgaaggaa cattgcttgc tcatacagac ggtgacgtgc ctaacttgct agtcaaaatt
    11821 ccagaaatgg atgcttatac attgggttat acaatgtact tctttgaaat tgctgtaggt
    11881 atttcaggtt acttgaatgg aatcaaccca ttcgaccaac caggtgtaga agcttacaag
    11941 aaaaacatgt tcgcacttct aggcaaacca ggctttgaag atcttgctaa agaattgaac
    12001 gaacgcctgt aagataaggg tttttcgcta gatttaaata atcagtgata gaaacatgtt
    12061 cgctttaaaa tgaaaactgg tacccattta ggtactcaaa catactgcat cgtttattca
    12121 gaataagggg ctgtgacaag acgtttgtca cagtcccttg tttgtataga aatcgattga
    12181 ttgccttgct ctttagtttg ttttaggact ttaagaacag tgaggctgac ttcgaaggtt
    12241 aattctgttt ttttgttttc tagaatcagt aagcaaaaaa gcactcactt cgagtgcttt
    12301 tttgcgatat attcttagct taagataggc ctaatttttc ttttacagat gagggtacct
    12361 tatcgctggt aattactttt ttctctatga tccgcttttc gcttgctttc aactgcaaga
    12421 tcgttccttc ggggtaagca gaaccattgg cagagtcacc agaagtaatg acatcgaaat
    12481 ctaactgtct tttttcaccg ttttcagcat aagcatctac gttgtattta aaacctcttc
    12541 caattacttc cccattatct gctttgatat cagtttctgc aggcattgga gctttgacta
    12601 ccgcataata atctttacct acataagtag actgatagta gtcccaacct ttccatccta
    12661 taaaagcaat caatatgata acgatcaacc ctaaaatttt tttcatgaaa gatctccttt
    12721 tttattttta ttatacccgt ggtgctagtt aattttaata tcctaaagaa tatctcaaag
    12781 ttccttcaaa acgtcgttgt gctcgactta atagaacact gtaagccagt aatatgcttt
    12841 caaatctact ctctagccct ttaacagaac gtgagttgaa attttgacaa cctaattttt
    12901 ctaaggaaga aaaaacagtc tcaactgt
//
LOCUS       DACEEZ010000082        12857 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20313_2017
            SAMN12639966-rid9879223.denovo.082, whole genome shotgun sequence.
ACCESSION   DACEEZ010000082 DACEEZ010000000
VERSION     DACEEZ010000082.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639966
            Sequence Read Archive: SRR10023158
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12857)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12857)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741683.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741683.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:44:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,016
            CDSs (total)                      :: 2,962
            Genes (coding)                    :: 2,889
            CDSs (with protein)               :: 2,889
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 36 of 73
            Pseudo Genes (incomplete)         :: 29 of 73
            Pseudo Genes (internal stop)      :: 18 of 73
            Pseudo Genes (multiple problems)  :: 8 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12857
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639966-rid9879223.denovo.082"
                     /isolate="E20313_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            6..305
                     /locus_tag="IWO89_002286"
     CDS             6..305
                     /locus_tag="IWO89_002286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAQ0457004.1"
                     /translation="MKNRLIVACGSGVATSQTIASKIQSMLEDDGIAFPVEAVDYKSI
                     QNELLTAGIYVYVAQPDEEVLEQAKDLGIEVFPGIPFLTGMGVEPIYDSIKELVQ"
     gene            378..1739
                     /locus_tag="IWO89_002287"
     CDS             378..1739
                     /locus_tag="IWO89_002287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIC"
                     /protein_id="HAQ0457005.1"
                     /translation="MEVFQTIINYVLNLGSAIFVPLIILILGLLAGMKLKKAFMSALT
                     LGIAFSGMSMVIGYMSNAVSPASEALAKNTGISLPALDLGWTGAASITWSWSYAFIFF
                     AVTIGVNFVLLLFNWTKTLNVDMWNVWGKALTAYLVYYVSGSLAAGFLTAMVQVILEL
                     KLGDMFQKHIQDLTGIPLVTVTHFMTSAAVLLLPFNMIMDKIPALNKRADTNALKKKI
                     GIFSENSVMGFIIGLGLGFAAAYGVSGSLNLAIQTATALSLFPMISKMFMQSLSPLAD
                     AMSEFMKKRFKDREVYIGLDWPILAGRSEIWVTAILLVPVFIGYAIILPGNQVLPLAG
                     IINYSIAVGGLLLTGGNLARMLILGIITMPIYLYGATYLAPILSDLAEKSGAVEGIKK
                     GQLITWSSIEGPEFRVLFAEAFNRNILAIVGCVIFLGLFVLLYKYMIKAKVPSERYAE
                     LQK"
     gene            1761..2021
                     /locus_tag="IWO89_002288"
     CDS             1761..2021
                     /locus_tag="IWO89_002288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0457006.1"
                     /translation="MKREETDKIKWTVALCGTLLLFLYGLFTQNIIINLLVIFFALVI
                     YKYGNHVLFREYDEKRKRKIEESMKIKEATKEILREKSFIKR"
     gene            2035..2787
                     /locus_tag="IWO89_002289"
     CDS             2035..2787
                     /locus_tag="IWO89_002289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016175975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldolase"
                     /protein_id="HAQ0457007.1"
                     /translation="MSDGRMLFERERQDMARIVRVIFERKNTNVAGGNFSFKTTDDQG
                     KDYIIMTPTMMSEAYLGEISPAQVLVVEPRTRRIVDGVGHLTREINMHEAMYETNPAI
                     KAVLHAHAPNAMFWATTGLPMPNLTEATAKVKEIPVMDFEPNCSEELAELTSDYVKKQ
                     HLELPHMLLLNSHGVLINTGGESGIEAIHKALQILDTVEWNAEIAYKQTIFQALGITD
                     GFYSKGEKIGGLEELKHKTAIYNKSVIAAGGD"
     gene            complement(2881..3486)
                     /locus_tag="IWO89_002290"
     CDS             complement(2881..3486)
                     /locus_tag="IWO89_002290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HAQ0457008.1"
                     /translation="MTYIFEKGTSGKHLILLHGTGGDEHSLLDIAHFLAPNSTLLSFR
                     GTVQEDGMNRFFKRNGLNQFDLDSLEKETDKLHEKIKEISEKELIPLSDWLLVGYSNG
                     ANIAAHLLLERETELNKGLFFHPMSLGVHQSRSTLEDKTVWISYGDNDPIVSPASFKE
                     LADQFETRQANVTIQLTVTGHQLTMDEIKQAREWTDHLLTK"
     gene            complement(3539..4513)
                     /locus_tag="IWO89_002291"
     CDS             complement(3539..4513)
                     /locus_tag="IWO89_002291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ring-cleaving dioxygenase"
                     /protein_id="HAQ0457009.1"
                     /translation="MDTQIRGIHHVTAMTSSATKIYRFFTDILGMRLIKKTVNQDDIE
                     TYHLYFTDDTGAPGTDMTFFDFPNQPKGKKGTDTISRASFRVPSDAALDFWLERFEKY
                     DVTHSAINERFGKKYIEFEDFDNQYYQLISDENNQGVAAGIPWKNSNVPTEFAITGLG
                     PVFVRVSQLEHIRLILEKILGFKETDHSGDFYLFEIGEGGNGASIIIEHRTDLPKAIE
                     GFGNIHHLALRVADKEALEYWIQVINKIELPNSGFVDRFYFQSEYFLAAHHVLFELAT
                     DGPGFLEDEPAESAGEKLSLPPFLEERREAIEAYVRPFDTSDANKKRE"
     gene            complement(4891..5670)
                     /locus_tag="IWO89_002292"
     CDS             complement(4891..5670)
                     /locus_tag="IWO89_002292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAQ0457010.1"
                     /translation="MLLRVVGSIIFRYTLEEVNNVPKYEEIANILRERIRSKEYPLDS
                     FLPNQVDLVKEFGASRMTIKKAINILAMEGLVYSQRGSGTKILNHPFMQKDTSPANEY
                     EGLSLQMKRRHRSLKSQIIHFDVEFPNQYIQERLMIDAEQPVYNIHRLRILDGEPYVL
                     EHTFMPVHLVPGLKKEHLLASIYDHLHQELNVHFAGAYRAISADKSDEYDQQYLNCAE
                     NDPVLEVQQVVYLKDGQPIEYSSSRNRFDVRNYSVLDVRST"
     gene            complement(5737..7008)
                     /gene="celB"
                     /locus_tag="IWO89_002293"
     CDS             complement(5737..7008)
                     /gene="celB"
                     /locus_tag="IWO89_002293"
                     /EC_number="2.7.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIC"
                     /protein_id="HAQ0457011.1"
                     /translation="MNGFMDKLSEKIMPLANLLGQNRYLTVLRDAFMLSFPLTMFGSI
                     VVVINNLPFFSDATKGTLSNLFGNGQNATMSIMSVFVTFGIGYYLSKSYDVEGIFGGA
                     VSFASFLILTPFAMKTAGGEDVTGVLSLDRLGAKGMFIGMIAAFLAGEIYCRITKRGW
                     QIKMPDGVPPAVTKSFAALIPAVVTLTIFLVINAVMTGIFNANLHDVVYEVIQKPLTG
                     LGSSLPATLIALFFVQFLWFFGLHGQIIVNSVMDPIWNTLMLDNLEAYQHGKELPHII
                     TKPFMETFTVGIGGSGMTLAVVLLMAFVLKKKQYRDVGRLALAPGIFNVNEPAIFGLP
                     IVLNATILIPWVVAPLIVTTFNYLVMAAGLVPAPTGVSVPWTVPIIASGVLATNSWLG
                     GMLQVIDFVIVAFIWYPFLKVLDKQPDLDIA"
     gene            7161..8558
                     /locus_tag="IWO89_002294"
     CDS             7161..8558
                     /locus_tag="IWO89_002294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HAQ0457012.1"
                     /translation="MKTYTFPENFWWGSAASGPQTEGRVSDDGKGENIWDHWYELEPE
                     KFFDQVGPEKTSQVYTKYKEDVQLMKETGHNTFRTSIQWSHLIPEGTGEINQKAVSFY
                     RDYFTELLDNGIEPFVNLYHFDMPLPLQEKGGWLSRETVDAYVSYAAACFELFGDLVK
                     KWFTHNEPIVPVEGGYLYQFHYPNHINMKEAVQVGYHENLASAKVIKAYHEGGHDGEI
                     GIILNLTPSYPRDETNPEDVKAAKIADAFFNRSFLDPAVKGEFPQELIEIVKELAIMP
                     EIHEGDLELIRENTIDLLGINYYQPRRIKAKETPINHEHSPMPDDYFDNYDMPNKKMN
                     PYRGWEIYEKGIYDILTNTRENYGNIKCYISENGMGVEGEERFVNSEGIIEDDYRIEF
                     VTDHLKYVHQAIQEGTNCVGYHMWTCMDNWSWTNAYKNRYGFIAVDLAQDGKRTIKKS
                     GRWFKTVSEKNGFDA"
     gene            8639..9079
                     /locus_tag="IWO89_002295"
     CDS             8639..9079
                     /locus_tag="IWO89_002295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HAQ0457013.1"
                     /translation="MVDTYRNILVAIDGSEKAEKAFLEAITIAKRNQATLHILYVNEV
                     TGNYFGDFAFVTTSLQEELDEVAENQMKEHRNLAIEKGLTDIETYVLYGYPKTLIANF
                     HESKEPIDLIVMGATGLNAVERALVGSTTSYVVNHASCNVMVVK"
     gene            9227..9553
                     /locus_tag="IWO89_002296"
     CDS             9227..9553
                     /locus_tag="IWO89_002296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HAQ0457014.1"
                     /translation="MISSDGIRGYNDAIILSILNKNDSYGYEISKTIRERTNDSYVIK
                     ETTLYSVFARLEKNQLIVSYPGEVTHGKKRTYYKITEIGRNLLEEKKQEWLTTKDVVD
                     QFLEGN"
     gene            9554..10303
                     /locus_tag="IWO89_002297"
     CDS             9554..10303
                     /locus_tag="IWO89_002297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0457015.1"
                     /translation="MDTIRNYLDSLFIGVPQSTEIDKLKTDLLANMEDHYHELMGEGK
                     NEQEAIGTVISTFGSIDELLEELDVEKKHQADETETNTASIYLSEAENYWKEYRAASL
                     QVASGVLFISLSFASFLFFCSAGYVFMGISCLIFGIALAVGFFIASGMKITRLNHFLH
                     HRKIPEKVLAEAKEKEEEYQRSFGFSLIAGIGLCIFSLFPLLASLMWYMDGSIGASIF
                     FVTVGTGVFLIIYGSLVRHSYRQFTQSAYYW"
     gene            complement(10383..10838)
                     /locus_tag="IWO89_002298"
     CDS             complement(10383..10838)
                     /locus_tag="IWO89_002298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spx/MgsR family RNA polymerase-binding
                     regulatory protein"
                     /protein_id="HAQ0457016.1"
                     /translation="MLTIYSSRTHSYHLMANWLKDHQMPYRERIFTPKQPLTRKEIFY
                     LLSLTENGFDDLLAKRSIDYKNLKDQIDQCTTSELVDLIVSHFTLLQRPIITDGKKLL
                     VGFNEDSMRLFIPVQWRRSQLKSVYNDFTWVSNLENSTDLDDEFLNYYI"
     gene            complement(11265..11540)
                     /locus_tag="IWO89_002299"
     CDS             complement(11265..11540)
                     /locus_tag="IWO89_002299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0457017.1"
                     /translation="MKLEDKKVILKKIKRLPDQIDGYTICSFKKDRSIKAYTQNTEKG
                     IIYFLHEEGYDTQTFMFTEKKEFHKQMKKLIEKEFPRSHKLYVNQQF"
     gene            11834..12649
                     /gene="thiD"
                     /locus_tag="IWO89_002300"
     CDS             11834..12649
                     /gene="thiD"
                     /locus_tag="IWO89_002300"
                     /EC_number="2.7.1.49"
                     /EC_number="2.7.4.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional hydroxymethylpyrimidine
                     kinase/phosphomethylpyrimidine kinase"
                     /protein_id="HAQ0457018.1"
                     /translation="MEDQYVQAMTIAGSDSDGSAGMQADMHTFFTRNVYGVSVMTACV
                     AGNSYGIGASVTLPTDFIDKEFELIAKDFQIRAAKTGMLADSKLIETVVKNYQKYDFG
                     PLVVDPVIVTKHGNLLLEESALQSLKEKLVPLAEVLTPNFYEAEKLTGRTLAAEEDFV
                     QAAKELQDMGAKNVMIKGHHAKNAQQETVNDYVLFESGKSFWLNAPFYPTDRINGTGD
                     SLSACIAAELAKGKTVEQAIRLAKDYVNRAIREEINVGHKYGPINHWAAGELE"
BASE COUNT         4121 a         2211 c         2625 g         3900 t
ORIGIN      
        1 aaaaaatgaa aaatcggtta attgtcgcat gtggaagtgg agtagcaacg agtcagacaa
       61 tcgctagtaa gatccaaagt atgttagaag atgacggcat cgcatttcct gtagaagcag
      121 ttgattacaa atcgattcaa aatgagcttc taactgctgg aatctatgtg tatgtcgcac
      181 aacctgatga ggaagtactt gaacaagcaa aagatttggg tattgaagta tttccaggaa
      241 ttccattttt aactggaatg ggtgtagaac ctatttatga cagtatcaaa gagttagtac
      301 agtaaaggag gattgttgga taaaaaagtt ggttcatagt tttatttact atatttagaa
      361 agaaagaagg tttatttatg gaagttttcc aaaccattat caattatgtt ttgaacttag
      421 gatcagccat ttttgttccc ctgattattt tgatacttgg attgttggca gggatgaaat
      481 taaaaaaagc attcatgtct gctttgactt tagggattgc ttttagcgga atgtctatgg
      541 tcattggcta tatgtctaac gcagtaagtc cagcatcaga agcattggct aaaaatacgg
      601 gaatcagttt gcctgcactt gatcttgggt ggacaggtgc agcaagcatc acatggtcgt
      661 ggagttatgc ttttatattt tttgcagtaa cgattggggt caattttgta ctgttacttt
      721 ttaattggac gaagacgtta aatgttgata tgtggaatgt ttgggggaaa gctttaactg
      781 cttatttagt ttattatgtt tccggtagtt tggctgcagg ctttcttact gcaatggtcc
      841 aagttatatt ggaattaaag cttggtgata tgtttcaaaa acatatacaa gatctcacag
      901 ggattccatt agtaacagtg actcatttta tgacatctgc cgctgtcttg ttgctgcctt
      961 tcaatatgat catggataaa attcctgctt taaataaacg agcggatacg aatgcattga
     1021 agaagaaaat tgggatcttc tctgaaaact ctgtaatggg attcattatt ggattggggc
     1081 ttggctttgc tgctgcgtat ggcgtttcag gttctcttaa tctagcgatc caaacagcta
     1141 cagcattaag tttgtttcct atgatctcta aaatgttcat gcaatctttg tctcctctag
     1201 cagacgctat gtcagaattt atgaaaaagc ggtttaagga tcgtgaagta tatatcggat
     1261 tggactggcc gatattagct ggtcgttcag aaatttgggt tactgctatt ttacttgttc
     1321 ctgtgtttat tggttatgcc attattttgc caggaaacca agtattgcca ttagcaggga
     1381 ttatcaacta ttcgattgcg gtcggcggat tacttttaac aggaggaaac ttggctcgta
     1441 tgctgatctt gggaatcatc actatgccta tttatttgta cggggccact taccttgcgc
     1501 caatactcag tgatttagca gaaaaaagcg gagcagtcga gggaatcaag aaaggacagt
     1561 tgatcacatg gtcatccata gaagggccgg agttcagggt tttatttgcg gaggcattta
     1621 ataggaatat tctcgctatt gtaggatgcg tgatttttct aggactgttt gttctacttt
     1681 ataaatatat gataaaagcg aaagtaccga gtgaacgtta tgctgaacta caaaaatagc
     1741 attgaaatgg tgggaaagta atgaaaagag aagagacaga taaaataaaa tggacagtcg
     1801 ctctttgtgg tacacttctg ctatttctat atggactctt tactcagaat atcatcatca
     1861 atctattggt catttttttc gcactagtga tctataaata tggaaatcac gttttattca
     1921 gagagtacga tgaaaaaaga aaacggaaaa tagaagaatc aatgaaaata aaagaagcta
     1981 ccaaagaaat attgagagaa aaaagtttta taaaaagata atggaggggt cattatgtca
     2041 gatggacgta tgttatttga gagagaacga caggacatgg cacgtatcgt aagagtgatt
     2101 ttcgaaagga aaaacacaaa tgtcgcagga gggaattttt cctttaaaac cactgatgat
     2161 caagggaaag actacatcat catgacgccg accatgatgt cagaagccta tctaggagag
     2221 atttctcctg cacaggttct agtagtagaa ccaagaacgc gtagaatcgt tgacggagtc
     2281 ggtcatttaa ctcgtgaaat caatatgcat gaagcgatgt atgagactaa tccagctatc
     2341 aaagctgtcc tacatgctca tgcgcctaat gcaatgttct gggcgactac tgggttgcct
     2401 atgcctaatt tgacagaagc cacagctaaa gtaaaagaga ttcctgtcat ggactttgag
     2461 cctaattgct ctgaagaact tgctgaattg acaagcgact atgtaaaaaa acagcatctc
     2521 gaattaccgc atatgttgct gctcaactct catggcgtat tgatcaacac aggtggagaa
     2581 tcaggaatcg aagctatcca taaagcactg caaatccttg atacagttga atggaatgca
     2641 gaaattgcct acaagcagac gatttttcaa gcattaggga ttactgacgg tttttattca
     2701 aaaggtgaga aaatcggggg attggaagag ttgaaacata aaacggctat ttataataaa
     2761 tctgttattg cagctggtgg agactaacct atctgacaca aaaaactacg actttcacaa
     2821 aatctgatcc agatcgatat ctgcttcagg ttgtattatg taagtcgtag ttcttttctt
     2881 ttatttagtt aagaggtggt ctgtccattc cctcgcttgc ttgatttcgt ccatcgtcaa
     2941 ctgatgacct gttacagtta gctgaatagt cacatttgct tggcgtgttt cgaattgatc
     3001 ggccaattct ttaaaggaag caggggaaac gattggatcg ttatcaccat aagaaatcca
     3061 gacggtttta tcctctaatg tggaacggct ttgatgaaca cctagagaca tcggatggaa
     3121 aaacaaccct ttatttagct cggtctcacg ctccaataat aaatgagcag cgatgtttgc
     3181 gccatttgaa tagccgacaa gcagccagtc acttaacggt atcagttctt tttcgctgat
     3241 ttctttgatt ttttcatgga gtttatctgt ttctttttcc aaactgtcca ggtcgaattg
     3301 attcaatcca ttacgcttga aaaaacgatt catcccatct tcttggaccg tgccacgaaa
     3361 agaaagcaat gtactgtttg gtgcaagaaa gtgagcgata tccagtaaag aatgttcatc
     3421 gccacctgtg ccatgaagta agatcaaatg cttgccactt gttccttttt caaaaatgta
     3481 ggtcattttg ttccctcttt tctgtttcta tagatgcaaa tgaagtagca tgaagagatt
     3541 attcacgttt tttatttgca tcacttgtat cgaaaggacg gacataagct tcaatggctt
     3601 cacgacgttc ttctaagaat ggaggaagag atagtttttc tcctgctgat tctgctggtt
     3661 catcttcaag gaatccagga ccatcagttg ctaattcaaa cagtacgtga tgagcggcta
     3721 ggaagtactc agattggaaa tagaaacgat cgacgaaacc agaatttggc aattcaatct
     3781 tattgattac ttgaatccag tattccaatg cttctttgtc tgctacacgc aatgctaagt
     3841 gatggatatt tccaaatcct tcgattgctt ttggcaagtc tgtccgatgt tcaataatga
     3901 tgcttgcacc atttccccct tcaccgattt caaataagta gaaatctcct gaatggtctg
     3961 tttctttgaa tcctaagatt ttttcaagaa tcaagcggat atgctctaat tgactgacac
     4021 ggacaaagac aggtccaagt cctgtgatcg caaactctgt cggtacattg ctgtttttcc
     4081 aaggaatgcc agctgctaca ccttgattgt tttcgtcaga gatcaattga taatactgat
     4141 tatcgaaatc ttcaaattcg atatattttt tgccgaaacg ttcattgatt gctgaatgag
     4201 taacgtcata tttttcaaaa cgttccaacc aaaagtctaa tgctgcgtca cttggcacac
     4261 ggaaagaagc acgagagatc gtatctgttc ctttttttcc ttttggctga tttggaaaat
     4321 caaagaaagt catatctgtt cctggcgcac cagtatcgtc tgtaaagtaa agatggtaag
     4381 tttcgatgtc atcttggtta actgtttttt tgattaagcg cattcctaaa atatctgtaa
     4441 aaaagcggta gatttttgta gcgctagatg tcattgctgt gacgtggtga attcctctga
     4501 tttgtgtatc catgataaat ttcctcctgt taacttacaa aaagtaagta gttgatttag
     4561 acatagtata gagggtgcat ctctattttg caattaattt gtcttctaaa gtatcaggaa
     4621 ggaatcaaga atggaaaaga ggagggacgg atcaataaaa taaaaagact tacgactagc
     4681 tgtatcgatt tcaaaaaaac tagctttaaa aagctaactt tagaatcatt acgggtggtc
     4741 ataagtcttt ttatgctgag ttattttttc atttttgagc taatgcttag gatagaatag
     4801 aatgttgatt tcacgatctc ttcttgttac ttgaaactgc ttaccactgt cacgatctct
     4861 tggtaaacgg ctttttcaca acctctttgg ttaagttgaa cgcacatcca gcacagaata
     4921 gttccgaaca tcaaatcggt ttcggctgga agaatattca atcggctgtc catctttgag
     4981 atagacgact tgctgaactt ccagtacagg atcgttttct gcacagttca aatattgctg
     5041 atcatattca tcacttttat ctgcagaaat agcacgataa gcccctgcaa aatggacatt
     5101 caattcttga tgaagatgat catagataga ggctaacaaa tgttcttttt ttaaacccgg
     5161 cacaagatgg actggcataa aggtatgctc taaaacatat ggctcgccat ctaagatcct
     5221 taaccggtga atattgtaga caggttgctc ggcatcgatc atcaatcgtt cttggatata
     5281 ttgattaggg aattcaacat caaaatgaat gatttgactc ttcaaagaac gatgtctgcg
     5341 tttcatttgg agactcaaac cttcatactc gttagcgggc gatgtgtctt tttgcataaa
     5401 tggatgattc aagattttcg ttcctgatcc tcgctgcgaa tagacaagtc cttccattgc
     5461 taagatattg atggcttttt tgattgtcat ccggctcgca ccaaattctt ttaccaagtc
     5521 gacttgatta ggcaaaaaac tatcgagagg atactctttt gaacggatac gttctcttaa
     5581 aatatttgcg atttcttcgt attttggcac gttattcacc tcttctagag tataacgaaa
     5641 gattatgctt ccgacaactc gaagtagcat ctggaacatt gtttattcga aaaaagaggt
     5701 catgacaagt ttttgtcaca accccttaat gatctattaa gcaatatcta aatctggttg
     5761 tttatctagt actttcagga atgggtacca gataaaggca acgatgacaa agtcgatgac
     5821 ttgcagcata ccaccaagcc atgaatttgt tgctaagaca ccgcttgcaa tgattggcac
     5881 agtccatgga acagagacgc cagttggtgc agggacaagt ccagcagcca ttaccaaata
     5941 attgaacgtc gttacgatta gaggtgccac aacccaagga ataaggattg ttgcattcaa
     6001 tacgatcggt agaccgaaga tcgctggttc atttacattg aaaataccag gtgctaaagc
     6061 taatcgtcca acatcacgat actgtttctt tttaagaaca aaggccatca ataaaacgac
     6121 agctaaagtc attccagagc caccaatacc aacagtgaag gtttccatga atggttttgt
     6181 gatgatgtgt ggtaattctt ttccatgttg ataagcttct aagttatcaa gcatcagtgt
     6241 attccaaatc gggtccatta cagagttgac gatgatttgt ccgtgaagtc cgaagaacca
     6301 taagaattgg acaaagaaca aagcaatcaa tgttgcaggt aaactacttc ccaaaccagt
     6361 taatggtttt tggatcactt cataaacgac gtcatgtaag ttagcattga aaataccagt
     6421 catcacagca ttgattacta agaaaatagt tagtgtcact acagcaggga tcaaagcggc
     6481 aaatgatttt gttactgcag gcggaactcc atcaggcatt ttgatctgcc agccgcgttt
     6541 cgtgattctg cagtagattt ctccagccaa gaaagcagcg atcattccaa tgaacattcc
     6601 ttttgcgcca agacgatcaa gtgaaagcac accagtcaca tcttcaccgc cagcggtttt
     6661 cattgcaaaa ggcgtaagaa tcaagaagct ggcaaaagac acggcaccac cgaaaatacc
     6721 ttcaacgtca taagattttg ataaataata gccgatacca aaagtaacaa aaaccgacat
     6781 gatgctcatt gttgcatttt gcccattacc gaataaattc gataaagttc cctttgttgc
     6841 gtcactgaaa aaaggcaagt tattgataac gacaacgatc gaaccgaaca tcgttaaagg
     6901 gaaggaaagc atgaaagcat cccgcagcac tgtaaggtaa cgattttgcc ctaataaatt
     6961 agctaagggc atgattttct cagaaagctt gtccataaaa ccgttcattt ttattcacct
     7021 caaatttatt tgcaatcgat tacatacaaa gtataacgcc aaaaatttta aatgtctagt
     7081 cattttcata aaaagtattg atttattttt tagggttcgt tatgattacg gtatcaagaa
     7141 aaaagagagg aagtaaaaaa atgaaaacct acacattccc agaaaatttt tggtggggtt
     7201 ctgctgctag cggaccgcaa acagaaggaa gagtaagtga tgatggtaaa ggagaaaata
     7261 tttgggatca ttggtatgaa ttagagcctg aaaaattctt cgatcaagta ggacctgaaa
     7321 aaacttcgca agtttacaca aaatacaaag aagatgttca gttaatgaaa gaaactggcc
     7381 ataacacatt tcgtacatct atccaatgga gtcatttgat tccagaaggg acaggtgaaa
     7441 tcaatcaaaa agctgtttct ttttatcgag attattttac tgaattgctg gataatggta
     7501 ttgaaccttt tgtgaacctt taccatttcg atatgccact accactgcaa gaaaaaggcg
     7561 gatggttatc aagagaaacg gtcgacgctt atgtttctta tgcagcagcg tgctttgaac
     7621 tgtttggaga tctggtcaaa aaatggttta ctcataatga accaatcgta cctgtagaag
     7681 gaggctactt gtatcaattc cactatccaa atcatatcaa tatgaaagaa gcggtccaag
     7741 taggctatca tgagaatcta gcaagcgcaa aggtaatcaa agcctaccat gaaggcggac
     7801 atgatggtga gatcggaatc atcttgaatc tgacaccaag ctatccgaga gatgaaacga
     7861 acccagaaga tgtaaaagcg gcgaagatcg ctgacgcatt cttcaaccga tcattccttg
     7921 accctgcagt gaaaggagag tttccacaag agttgatcga aattgtcaaa gaattggcta
     7981 taatgccaga gatccatgaa ggagacctag aactcatccg tgaaaacacg attgacctgc
     8041 taggaatcaa ttactatcag cctcgtcgaa tcaaagcaaa agaaactcca atcaatcacg
     8101 aacatagccc catgccagat gattattttg ataattacga catgcccaat aaaaaaatga
     8161 atccatatcg tggatgggaa atttacgaaa aaggaatcta tgatatttta acgaatacaa
     8221 gagaaaacta tggtaatatc aaatgctata tttctgaaaa tgggatgggg gtcgaaggag
     8281 aagaacgatt tgttaacagc gaaggaatta ttgaagatga ttaccggatc gagtttgtta
     8341 cggatcattt gaaatacgtc catcaagcaa tccaagaagg aaccaattgt gtgggttacc
     8401 acatgtggac atgtatggac aactggtctt ggacaaatgc ttataagaat cgctatggct
     8461 ttatcgctgt tgatttggca caagacggta aacggacgat caaaaaatca ggtcgctggt
     8521 ttaaaacggt ctctgaaaaa aacggatttg atgcctgata ttttttgata aaacgttcac
     8581 aaaatgtttg ttctaacact gtaatcgtgc tacactaaca gtgaaggagg cgtttacaat
     8641 ggtagatact tatcgtaata ttttagtagc aattgatggt tctgaaaagg cagaaaaagc
     8701 atttcttgag gcaatcacga ttgccaaacg aaatcaggca acgttgcaca tcctgtatgt
     8761 caatgaagta acaggcaatt actttggtga ttttgcattt gttacaacaa gccttcagga
     8821 agaattggat gaggttgcgg aaaaccagat gaaagaacat cgaaacttgg caattgaaaa
     8881 aggcctgaca gatatcgaga catatgttct ttacggttat ccaaagacac tgattgctaa
     8941 tttccatgaa tcgaaagaac caatcgatct gattgtcatg ggggcgacag gtctgaatgc
     9001 ggtagaacgt gcactagtag gttccactac ttcctatgta gtcaaccacg catcatgtaa
     9061 cgtcatggtt gtaaaataaa taaattaagg gaacagaaag caaaaaagca taacgcttct
     9121 tttgctttct gttcctttta tttttttgta aaaaattact ttacctgatg aagtaatcat
     9181 gctattctaa ttacaacgac agatggagta atatggagga aagaaaatga tttcaagtga
     9241 tggaatacgc ggatacaatg atgcgattat tttatcgatc cttaacaaaa acgattctta
     9301 tggatatgaa atttctaaaa cgattcgtga gaggacaaat gattcctatg tgatcaaaga
     9361 gacaacactt tactccgttt ttgcccgttt agaaaagaat caactgattg tttcgtatcc
     9421 tggagaagtg actcatggaa aaaagcggac atattataaa atcactgaaa tagggcgtaa
     9481 tcttttagaa gaaaaaaagc aagagtggct gacgacgaaa gacgtagtcg atcaattttt
     9541 ggagggaaat taaatggata caatccgtaa ttatttagac agtcttttta tcggagtacc
     9601 tcaaagtacc gagatagata agctgaaaac tgatttgttg gcaaatatgg aagaccatta
     9661 tcatgaattg atgggagaag gaaaaaatga acaggaggcc atcggtacag taatttcgac
     9721 atttggttca atcgatgaat tattagaaga actagatgtc gaaaaaaaac atcaagctga
     9781 cgagacggaa acaaacacag cttctattta tttaagcgaa gcagaaaact actggaaaga
     9841 atacagagcg gcaagtctcc aagtggcatc aggtgttcta ttcattagct tatcttttgc
     9901 aagttttctt ttcttttgtt cagcaggata tgtgttcatg ggtatttctt gtctgatttt
     9961 cggtatagca ttagctgtcg gtttttttat tgcttctggt atgaaaatca cacgtttaaa
    10021 tcactttttg caccatcgga aaatacctga aaaagtatta gcagaagcaa aagaaaaaga
    10081 agaagagtat cagcgaagtt ttggcttctc actgattgca ggaattggat tatgtatttt
    10141 ttcacttttc ccacttttgg cttctttaat gtggtatatg gatggtagta tcggtgcctc
    10201 tatatttttt gttacggtag gaacgggtgt cttcttgatc atttatggca gtctagtccg
    10261 tcattcttat cgtcaattca ctcaaagcgc ttattactgg tgaatgtatt cagatttgta
    10321 aaaatattct atttatccgg cgtagaaaaa aagactgtga cacgcttttg tcacagtctt
    10381 ttttatatat aataatttaa aaattcatca tctaaatccg tagaattttc tagattgctt
    10441 acccatgtaa aatcgttata gacagatttc agttgacttc tgcgccattg tacagggata
    10501 aaaagacgca tgctatcttc attgaatccg actaataatt tctttccgtc agtaatgatg
    10561 ggacgttgta acaaagtaaa atgcgagacg atcagatcga caagctctga ggtcgtacat
    10621 tggtcgattt ggtctttcaa atttttataa tcaatcgagc gtttagctaa tagatcgtca
    10681 aatccgtttt ctgttaatga caataaataa aatatctctt tccttgttaa aggctgttta
    10741 ggtgtgaaaa ttcgttctct gtaaggcatt tgatggtctt tcagccagtt tgccatcaga
    10801 tggtaactat gtgtgcgaga agaatagatc gtaagcataa aaaaactcct taaatagtcc
    10861 attaatttgt tagatctgtt gagataaata ataggtaagg agctgtgcat aagcattgat
    10921 caaaacaacc caaacatcat tagaaataac tttctaaagt cttaaacttg cgacgagaaa
    10981 caactagact aatagaaaaa atgtcttagc catttggctt cctgtttgtt tttttgctta
    11041 tgctgcagct cctcgggaaa gggttctaac aatttgaaga aggttgatga ttgatctagt
    11101 ttaaattgtg tgaaatttga aatctggtgt ttttactaga tgaatcacct aacaactatg
    11161 attataactt atagaacttc tgttttgcat tcggttttgt tatagttgtt ataaagtttt
    11221 attttattac agaacatttt taattaataa agaacagtta gaagttaaaa ttgctgattc
    11281 acatagagtt tatgacttct tggaaattct ttttcaatca gttttttcat ctgtttatga
    11341 aattcttttt tttcagtaaa cataaaggtt tgggtatcgt aaccttcttc atgtaggaaa
    11401 tagattattc ctttttctgt gttttgcgta taagctttta tactcctgtc ttttttgaaa
    11461 gaacagatcg tatagccatc tatttggtct ggtagtcgtt tgattttctt caaaatgact
    11521 tttttatcct caagtttcat ttttttcacc tgactttatt tttttcatat ggaaatcata
    11581 gcgcctttgg ttatatgaaa acaagaagga tactgaaata tatataaatc aaatagtggt
    11641 tatttattat tagaatagcg aatacaaata aacgaccact attttctgat ttattgcaaa
    11701 tgttttttca aataaatttg attaccaaga aaaggtagtg gtatatttac tgagggatac
    11761 tttgaaaaac atatgataga aacagtaaca gtattatgaa aaatcttttt ataaaaatgg
    11821 aggaatcgat ttgatggaag atcagtatgt acaagcaatg acgatcgcag gatcagatag
    11881 cgatggaagc gcagggatgc aagcagatat gcatacattc tttacaagaa atgtttacgg
    11941 tgtttctgtc atgaccgcat gtgttgcagg taattcttac ggaatcggag caagtgtgac
    12001 attgccaaca gattttatcg acaaagaatt tgaactgatt gcgaaggact ttcaaatacg
    12061 tgcagctaaa acaggtatgc tagcagattc aaagttgatt gagacagttg taaaaaatta
    12121 ccaaaaatat gattttggac cattagtcgt ggatcccgta atcgtaacga aacatggaaa
    12181 tcttttacta gaagaatctg ctttgcaaag cttgaaagaa aagcttgtcc ctttggccga
    12241 agtactgaca cctaattttt atgaagcaga aaagctgact ggacggacat tagcagctga
    12301 agaagatttt gtgcaagcag caaaagaact gcaagatatg ggtgcaaaaa acgttatgat
    12361 caaaggacat catgctaaaa atgcgcagca agaaacagtc aatgactacg tattgttcga
    12421 atcaggaaaa tcattttggt taaatgcacc attttatcct accgaccgga tcaacggaac
    12481 tggcgattcg ctatctgcat gtattgcagc tgagttagcg aaaggaaaaa cagttgaaca
    12541 agcgatccga ttggcaaaag actatgtaaa ccgtgcaatt cgtgaagaaa taaatgtagg
    12601 gcacaaatat ggccccatca atcattgggc agctggtgaa ttagagtaaa agaaagaagt
    12661 tgtgaaaaag ttgccctgca tcaagtaata agaacagcca agtaaaaaca gcttcttatg
    12721 tttttaactt ggttggactt attaccgcag atgcaagctt ttgaacaccg tttattcgga
    12781 aaaagagcgt gggacaaaag taaagaacac ttttgtctca cgctctaaac acgtataaac
    12841 ggcggaagca gaagaaa
//
LOCUS       DACEEZ010000083        12732 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20313_2017
            SAMN12639966-rid9879223.denovo.083, whole genome shotgun sequence.
ACCESSION   DACEEZ010000083 DACEEZ010000000
VERSION     DACEEZ010000083.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639966
            Sequence Read Archive: SRR10023158
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12732)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12732)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741683.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741683.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:44:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,016
            CDSs (total)                      :: 2,962
            Genes (coding)                    :: 2,889
            CDSs (with protein)               :: 2,889
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 36 of 73
            Pseudo Genes (incomplete)         :: 29 of 73
            Pseudo Genes (internal stop)      :: 18 of 73
            Pseudo Genes (multiple problems)  :: 8 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12732
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639966-rid9879223.denovo.083"
                     /isolate="E20313_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(85..264)
                     /gene="rpmF"
                     /locus_tag="IWO89_002301"
     CDS             complement(85..264)
                     /gene="rpmF"
                     /locus_tag="IWO89_002301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HAQ0457019.1"
                     /translation="MAVLARRTSKAKKAKRRTHYKLTIKGLNECPNCGEMKKSHHVCA
                     NCGYYDGKDVMSKEA"
     gene            complement(322..891)
                     /locus_tag="IWO89_002302"
     CDS             complement(322..891)
                     /locus_tag="IWO89_002302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF177 domain-containing protein"
                     /protein_id="HAQ0457020.1"
                     /translation="MKWSLLELRKYQETPLTFHETLDLKAALMKRDNLILDVAPVEVE
                     GLVSVDKKGYIVHYTVQTTLTVPSTRSLEPVELPMDFSVDEVFMTEEQFQSRDEQMSA
                     DEILLIEGPLLNLAESIEDNILLAIPMRVLTKEEEAATELPKGNDWEVVSEEEYQRRK
                     MEEAENKIDPRLAKLSELFDSKSKDDDNK"
     gene            1077..1979
                     /locus_tag="IWO89_002303"
     CDS             1077..1979
                     /locus_tag="IWO89_002303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HAQ0457021.1"
                     /translation="MNTKDIEYYVQIVKQKNFTKVANYFRVSQPTITYALQRLEKEFD
                     TVLITRDRSHHELIVTPSGKQLFLHAQTILQEISTARAEITQLQKKKLRFGMPPIIGN
                     EYFPKLSSYFIKHELMNQIEITDGGSRDLYGMIRQGRIDLAVLGSTQPIVDQYLETEL
                     LLDKRFMIVVPPTHPLSKQDTVSFKDIADEQFVLLNEHYVHPAAFTKLSKQTHTHPKV
                     VYQSNDLTILKSMVRENVGIGFLAEIAVHPEDHLVCLPLSDEVQPRFLISLAKRTQQI
                     VTPLQEQVVQVIHEFVTAQKNSSS"
     gene            complement(2014..3798)
                     /gene="pyk"
                     /locus_tag="IWO89_002304"
     CDS             complement(2014..3798)
                     /gene="pyk"
                     /locus_tag="IWO89_002304"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HAQ0457022.1"
                     /translation="MKKTKIVSTLGPASNSVEIISQLIESGANVFRFNFSHGDHEEQL
                     SRMTMVREAVKKTGKDVGILLDTKGAEIRTTVQGTTEADFGRAGYIKFEVGDQTRISM
                     DPEHVGSKEKIAVTYPGLFDDVHVGGHILFDDGLIDMQIIEKDEANRELVVEVKNAGM
                     LGSRKGVNAPGVSISLPGITPKDADDIRFGLDNDIDFIAASFVRKAQDVLDIREILEE
                     KDMTHVQIFPKIESQEGIDNIDEIIKVSDGVMIARGDMGVEIPAELVPMVQKRIIKKC
                     NAAGIPVITATQMLESMQENPRPTRAEASDVANAVFDGTDATMLSGESANGDYPVEAV
                     STMARIDQEAENALSELGTFQLNEFDKTDVTETIGLSVARAAKNLGVKTIVAATESGY
                     TAKMISKYRPDADILAVTFDERTKRGLMLNWGVYPTVAEKPSTTDEMFELAAKKAVEL
                     GFASEGDLILITAGVPVGERGTTNVMKIQMIGSKLLEAQGVGQKSVVANAVIASSAEE
                     AVKKAKDGMVLVVPSTDKEYMPAIEKAAAVIVEEGGLTSHAAVVGIAQDIPVIVGAKG
                     ATTTITEGELITVDSRRGIVYRGETTAI"
     gene            complement(3872..4834)
                     /gene="pfkA"
                     /locus_tag="IWO89_002305"
     CDS             complement(3872..4834)
                     /gene="pfkA"
                     /locus_tag="IWO89_002305"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HAQ0457023.1"
                     /translation="MKRIGILTSGGDAPGMNAAVRAVVRKGIYEGLEVYGINYGFAGL
                     VAGDIRRLDVADVGDKIQRGGTFLYSARYPEFATEEGQLKGIEQLKKFGIEGLVVIGG
                     DGSYHGAMALTKRGFPAVGIPGTIDNDIPGTDFTIGFDTAINTVLESIDRIRDTATSH
                     VRTFVIEVMGRNAGDIALWSGVAGGADEIIIPEHDFDMASVAKKIQEGRDRGKKHCLI
                     ILAEGVMGGNEFAEKLSEFGDYHTRVSILGHVVRGGAPSARDRVLASKFGAYAVELLQ
                     EGKGGLCVGIHDNEIVASDIIDTLENNKHKPDLSLYDLNNSLSF"
     gene            complement(5002..8316)
                     /gene="dnaE"
                     /locus_tag="IWO89_002306"
     CDS             complement(5002..8316)
                     /gene="dnaE"
                     /locus_tag="IWO89_002306"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HAQ0457024.1"
                     /translation="MFFPQLYTITSYSLLQSTIRIREYVQEAKKQGYTSLAITDKDVL
                     YGAPEFYRICVEEGVQPIIGLHLSYTYEEKNYGLLAYAKDEIGYQQLMKLSSRKMIQE
                     KLELAEMTETDHLAFVVPANHSVSEAFNKEDNFFGHWQFLLENVFTRNFYIGLTENTP
                     DQLPEWYQYVIKENYSLCALHPIASLQQEELFALEVMKHIKEGSQIPLENLSASLPLT
                     ANVYLLSEQEMIKRFEKAGLSQAVIGAQRLAESISFEFQFHQKLLPHYPVPNGKQAGE
                     YLRELCYSQVERRITDYTKEYQERLDYELSVIHQMGFDDYFLIVWDVMAFAHRNKIVT
                     GAGRGSAAGSLVAYVLEITDVDPLEYDLLFERFLNPERFTMPDIDLDIPDNRREEVLN
                     YVREKYGQYHMAQIATFGTMAAKMVLRDAARVFGLSQSEANRWSKAIPNQLKITLTQA
                     YKTSHQLVELVQASEKNQLLFKTAVILEGLPRHVSTHAAGVVISDKNLLELVPLQRGS
                     DEAFLTQYTMNEVEAIGLLKMDFLGLRNLSIIDFTLKAVKRVEGYEIRLKEIPLNDAK
                     TLLLFQRGETSGVFQFESAGIRNVLRRLDPENIEDIAAVNALYRPGPMQNIDTFIARK
                     KGKEAIRYPDDSLIPILKNTYGVIVYQEQIMQIASTMAGFTLGQSDILRRAVSKKKKD
                     VLDEEKSHFVSGALQQGYSEKTAATVYEYIEKFANYGFNRSHAFAYSFVGFQMAYLKA
                     HHPGAFFTSLMNSVRHNTSKLKEYIAEARKNKLKLSSPSINQSNSSFELVNKEEIRFG
                     LTAIKGIRRDFVEDILNERKQNGPFQSVDQFLIRIDKRWLKLELLQSLTAVGVFDELS
                     PNRKQLMLDLEGKIQNIVYSGGSLDLLGIMALKEEEVADYTLEERLKLEEEYLGIYLS
                     GHPAEGFDRLRLAKQVQLITDIVPNQPVNLLVMIKEVREIRTKKGELMAFVEGTDSSG
                     EISVTVFPTLYRKYRNLFAVDRVIFVQGRSEISKYNQELQLIGESFADPNQLEAQYPS
                     QTCYLRITEEREDPEIMKQLQVIFQKHPGYIPVVMYHEKTQRKVVLSDAYWVDDSKSI
                     KGQLIYLLGEDNVVFK"
     gene            8547..8732
                     /locus_tag="IWO89_002307"
     CDS             8547..8732
                     /locus_tag="IWO89_002307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjzD family protein"
                     /protein_id="HAQ0457025.1"
                     /translation="MRYIVTLFWAVVLGQVVGYIGAALTSGTYDFTLTTIISFIAGVI
                     ILLIGAVAPRKETSAHS"
     gene            complement(8794..9990)
                     /locus_tag="IWO89_002308"
     CDS             complement(8794..9990)
                     /locus_tag="IWO89_002308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter"
                     /protein_id="HAQ0457026.1"
                     /translation="MEINWRKNLVIAWIGCFFTGASISLVMPFIPVYVEQLGTPKDQI
                     ELFSGLAISVTAFASAVVAPIWGNLADRKGRKLMMIRAAAGMTLTMGSLAFVPNAYWL
                     LIMRFWNGILSGYIPNATAMIASQAPREKSGWALGTLSTGAIAGNLIGPSMGGALAQW
                     FGMENVFLITGALLMITTVLTIFLVKEDFHPIEKKDLISTKEIFSKMDHLSILIGLFI
                     TTLILQIGITTISPILTLYIRELSGSTENILFVSGLIVSIAGVSAVFSSPKLGKLGDK
                     IGNQKVLIGGLILSFCCYLPMAFVTTPLQLGILRFILGFSTGALMPSVNTLISKITPQ
                     EGVSRIYSYNQMFSNFGQVLGPMLGSTVARAYNYSAVFIVTSLFVLSNILLSLFNFRK
                     VLRSRL"
     gene            complement(10160..11596)
                     /locus_tag="IWO89_002309"
     CDS             complement(10160..11596)
                     /locus_tag="IWO89_002309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NCS2 family permease"
                     /protein_id="HAQ0457027.1"
                     /translation="MEKFFGLKKNNTNVSTEIMAGVTTFFAMSYILFVNPTILSASGM
                     PFQAVFLATIIASIIGTLVMGLFANVPYAQAPGMGLNAFFTFTVVFGLGYSWQQALAM
                     VFICGLINIFITVTNIRKMIIRAIPESLQHAIGGGIGIFVAYVGIKNAGFLSFSADQS
                     AISSSVVEGGKATNVTINGGIVPALANFDNAPILLAVIGLVLTAILVVKNVRGAILIG
                     IVATTVLGIFMGVVDLSSIDWQTNSLGNSFKELGTTFGAAFGSEGMQSLFSDSSKIPQ
                     VLMTIIAFSLSDTFDTIGTFIGTGRRTGIFSKEDEIALEDGRGFKTKMDKALFADAIA
                     TSVGAIFGTSNTTTYVESAAGIGAGGRTGLTSVVVAVLFALSSLFSPLIAIVPNQATA
                     PALILVGVMMMASFADIKWLDMEEALPAFFASIFMGLCYSISYGIAAGFIFYTIVKVI
                     KGKYNQVSVALWIVDILFIINFIILATI"
     gene            11889..12698
                     /locus_tag="IWO89_002310"
     CDS             11889..12698
                     /locus_tag="IWO89_002310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HAQ0457028.1"
                     /translation="MNKKLIAIDLDGTTLNQDSMITSKTKETLKKAINAGHHVSIATG
                     RPFRMSHQFYQQLELTTPMVNFNGALVHIPNQHWDGERETLINREIAFEILSQKKQLN
                     LDFIAAENRDTFFIDSFDFFDEKIFASSRPGEKNLLSPKNLTTNPTSLLVRTDKRFAG
                     AVSAELTRQFGSYVDVRTWGGPTAILEIVSKGIQKAKGVQEIANYLSIDQKDVIAFGD
                     EHNDLELLDYAGWGVAMANGTDQLKGIANDVTPLSNQEDGLAVYLEKLLKL"
BASE COUNT         3802 a         2673 c         2249 g         4008 t
ORIGIN      
        1 attttttatt taccgactac aaaaaaataa acctcaaggc tccatggtta gccttgaggt
       61 tttacatttt ctaattaaaa tgtattaagc ttcttttgac attacgtcct taccatcata
      121 gtaaccacaa tttgcacata cgtgatggct ttttttcatt tcaccacagt ttggacattc
      181 gtttaaccct ttgattgtca atttgtagtg tgtacgacgt tttgcttttt tagcttttga
      241 tgttcttcta gctagtactg ccattttgct acacctcctt gtaaagtctt gtatctaaat
      301 tagacacaat atattccaat cttacttgtt atcgtcgtct tttgatttag aatcaaaaag
      361 ttcagatagt tttgcaagac gaggatcaat tttattctcg gcttcttcca tttttctgcg
      421 ctggtattct tcttcggaaa caacttccca atcattgcct tttggaagtt ctgttgctgc
      481 ttcctcttct tttgtcaata cccgcattgg aatcgccaat aagatgttgt cttcaatcga
      541 ctctgctaaa ttcaataatg gaccttcaat tagcaaaatc tcatctgcag acatttgttc
      601 atctctactt tggaattgtt cttctgtcat gaatacttcg tctacagaaa aatccatggg
      661 cagttcaaca ggttctagtg aacgtgttga aggaactgtc aaagtcgtct gcacagtgta
      721 atgaacgata tagcctttct tatcaactga gaccaaacct tcgacttcca ctggcgcaac
      781 atcaagtatt aaattgtctc gcttcatgag agcggctttc aagtcgagcg tctcatgaaa
      841 agttaacggt gtctcctggt attttctcaa ttccaataaa gaccatttca tttttcatca
      901 ctcctaagca acaaaagcaa ttatacaggg caaaaagcat gttgtcaatg aaattttctt
      961 gacaccttgc agttcataaa aaaacagaaa aatctttatt agaaagcctc tgtttgctat
     1021 catcctcaga atcatttaca ataatcatta ttccattaaa ggagcggatg atcttcatga
     1081 acacgaaaga tatcgaatac tatgtccaaa tcgtcaaaca aaaaaatttt acgaaagtgg
     1141 ctaattattt ccgtgtgagc caacctacta ttacatatgc attacaacgt cttgaaaaag
     1201 agtttgacac cgtacttatt acaagagatc gatcgcatca cgaattaatc gtcacgcctt
     1261 ctggaaagca gctgtttctc catgcacaaa ccattctcca agagatttct actgcaagag
     1321 ctgaaatcac tcagttgcaa aagaaaaagc ttcgttttgg catgcctcct attatcggca
     1381 atgaatactt tcctaaactg tcaagttact ttatcaagca tgaattgatg aatcaaattg
     1441 agattaccga tggaggctct cgggacttat acgggatgat ccgacaaggg aggatcgatt
     1501 tagctgtatt aggctcaaca cagccaatcg tggatcaata tttagaaaca gagttacttt
     1561 tggacaaacg tttcatgatc gtcgtgccac ctacacatcc tctttccaag caggatactg
     1621 tctctttcaa agatatagca gatgaacagt ttgtactatt gaacgaacat tatgtccacc
     1681 ccgcagcatt cactaagctt tctaaacaaa cacatactca tccaaaagtt gtttatcaaa
     1741 gcaatgactt gacgatttta aagagtatgg tccgagaaaa tgtgggtatc ggttttttgg
     1801 cagagattgc tgtccatcct gaagatcatt tagtttgcct ccctctttca gatgaagttc
     1861 agcctcgctt cttgatttca ttggcaaaac gaacacagca gatcgtcacg cctcttcaag
     1921 aacaagtagt ccaagttatc catgaattcg ttacagcaca aaaaaacagc tcttcgtaaa
     1981 agaagagctg tttttatttt ttattaagct aaattagata gctgttgttt cgccacggta
     2041 aacgatgccg cgacgtgaat caacggtaat caattcacct tctgtaattg ttgttgttgc
     2101 gcctttagca ccaacgatta caggaatatc ttgtgcaatt cctacaactg cagcgtgaga
     2161 agtcaatccg ccttcttcaa cgataacagc agctgctttt tcgattgcag gcatatattc
     2221 tttgtcagtt gatggtacaa ctagaaccat tccgtctttt gcttttttta cagcttcttc
     2281 agcagatgaa gcaataactg catttgcaac gacagatttt tgtccaacgc cttgagcttc
     2341 taataattta gaaccaatca tttgaatttt catcacgtta gttgttccgc gttcaccaac
     2401 tggtacacca gcagtgatca agattaaatc gccttctgat gcgaaaccta attcaacagc
     2461 ttttttagct gctagttcaa acatttcatc agttgttgat ggtttttcag caactgttgg
     2521 gtaaacgccc cagtttaaca tcaaaccacg ttttgtgcgt tcgtcaaaag taacagccaa
     2581 gatatctgca tctggacgat attttgaaat catttttgca gtgtatcctg attcagttgc
     2641 agcaacgatt gttttcacgc ctagattttt tgcagcacga gctacagata aaccgattgt
     2701 ttcagttaca tctgttttat cgaattcgtt caattggaat gtacctaatt ctgataatgc
     2761 attttcagct tcttggtcga tacgtgccat tgttgaaaca gcttcaactg ggtagtcccc
     2821 gtttgcagat tcaccagata gcattgttgc atctgtgcca tcaaatacag cgttagcaac
     2881 gtcagaagct tccgcacgtg ttggacgtgg gttttcttgc attgattcta acatttgtgt
     2941 agcagtgata actgggatac cagcagcatt acattttttg ataatgcgtt tttgaaccat
     3001 tggtactagt tcagctggga tttcaacacc catatctccg cgggcaatca taacaccgtc
     3061 agaaactttg atgatttcgt caatgttgtc gataccttct tgagattcga ttttagggaa
     3121 gatttgaaca tgagtcatgt ctttttcttc taagatttcg cggatatcta gaacgtcttg
     3181 tgctttacgt acaaaacttg cagcgataaa gtcgatatcg ttgtctaaac caaaacgaat
     3241 atcgtcagca tcttttggag taatacctgg caaactaata gaaacgcctg gtgcatttac
     3301 ccctttacgt gatcctagca tacctgcatt tttaacttct acgactaatt cacggtttgc
     3361 ttcgtctttt tcaatgattt gcatatcgat caaaccatca tcaaataaaa tgtgtccgcc
     3421 aacgtgaaca tcgtcaaata atcctgggta agtaaccgcg attttttctt ttgaacctac
     3481 atgttcagga tccatagaga tgcgtgtttg atctcctact tcaaatttga tgtatcctgc
     3541 acgaccaaaa tcagcttctg ttgtgccttg aacagttgta cggatttcag ctccttttgt
     3601 atctaaaaga ataccgacat cttttcctgt ctttttaact gcttcacgga ccatagtcat
     3661 acgtgataat tgttcttcat ggtcaccatg agaaaaatta aagcggaaga catttgcccc
     3721 agattcaatc aattgagaaa taatttctac tgagttactt gctggtccta atgtactaac
     3781 gattttggtt tttttcattt aaaaaactct cccattcaat tttgtttatt taaagctgta
     3841 cacagcttaa atagcttggt ttctttcact cttaaaatga aagtgaatta tttagatcat
     3901 atagtgataa gtctggttta tgtttgttgt tttcaagtgt atcaatgatg tcagacgcaa
     3961 cgatttcgtt gtcatggata ccgacacata atccaccttt tccttcttga agcaattcga
     4021 cagcgtaagc accgaattta cttgccaaca cgcggtcgcg tgcgcttggt gctccaccac
     4081 ggacaacgtg tcccaagata gatacacgtg tatgatagtc accaaattct gaaagtttct
     4141 cagcgaattc atttccgccc attacacctt cagccaagat gatcaagcaa tgtttcttcc
     4201 cgcgatcgcg gccttcttgg atttttttag caactgaagc catatcaaaa tcatgttctg
     4261 ggatgatgat ttcatccgct ccgccagcca cacctgacca taaagcgatg tctcctgcat
     4321 tgcgtcccat tacttcgata acgaatgtac gaacatgaga agtcgctgtg tcacgaatac
     4381 ggtcaattga ttctagtact gtattgattg ctgtatcaaa accgattgtg aaatcagtcc
     4441 caggaatatc attatcgatt gttccaggaa tacctactgc tgggaaaccg cgttttgtca
     4501 aagccattgc gccatgataa gagccgtctc ctccgataac cactagacct tcgattccga
     4561 attttttcaa ttgttcgatt ccttttaatt gaccttcttc agttgcaaat tctggataac
     4621 gtgcagaata caagaatgta ccaccacgtt ggatcttgtc acctacatct gccacatcaa
     4681 gacgacgaat atctccagca actaaaccgg caaaaccata attgatcccg tatacttcca
     4741 gtccttcgta aataccttta cgaacaacgg cacgaacagc tgcattcatt ccaggtgcgt
     4801 ctccgccact ggtcaaaatt ccgatgcgtt tcataataaa cattcacctc attaaaatac
     4861 tttatcctaa gtatgactta ggtttacaac catttttgcg ttaaccacga ctaacattct
     4921 aacattattt ttctcacttg tcttgattta cggataaaaa aatgaaaaaa aatgaaagca
     4981 agtagtattt tttaccgtaa gttatttgaa aaccacatta tcttcgccaa gcagataaat
     5041 aagttgcccc tttatactct tgctgtcatc cacccaataa gcatctgaca gaactacttt
     5101 tcgttgcgtt ttttcgtgat acataactac tggtatatat cctgggtgtt tctgaaaaat
     5161 cacttgtaat tgtttcatta tctcgggatc ttcccgttct tctgtaatac gcaaatagca
     5221 agtttgtgaa ggatactggg cttctaactg gttaggatca gcaaagcttt ctccaattag
     5281 ttgcagttcc tgattatatt tgctgatttc gctccgccct tgaacaaaga tcactcgatc
     5341 aacggcaaac aaattacgat atttacgata aagagttgga aaaactgtga cagaaatttc
     5401 tccactactg tcggttcctt caacaaaggc catcagttct cctttttttg tccgaatttc
     5461 tctaacttcc ttgatcatca ctaataaatt gactggttga ttagggacga tatctgtaat
     5521 cagctgaact tgtttagcta aacgaagacg atcaaaccct tctgcaggat gacctgacaa
     5581 ataaatacct aagtactcct cttccagctt caagcgttct tctagtgtat agtcagcgac
     5641 ttcttcttct tttaatgcca tgattcccag caagtcaaga cttccaccac tgtaaacgat
     5701 attttggatt tttccttcga gatcaagcat caattgtttc ctgttaggag ataattcatc
     5761 aaaaactcct actgctgtca gtgattgcag taactctaat ttcagccaac gcttatcgat
     5821 ccgtatgagg aattgatcca ctgactggaa cgggccattt tgttttcttt cgtttaatat
     5881 atcttctaca aaatcacgcc gaataccttt tatcgctgtc agtccgaatc tgatttcttc
     5941 cttattgacc aactcaaaac tggaattact ttgattaatg gacggcgagg aaagtttgag
     6001 tttattttta cgtgcttctg caatatattc tttcaatttt gatgtgttat gccgaacaga
     6061 attcatcagg ctggtaaaga aagcacctgg atgatgggct tttagatacg ccatttggaa
     6121 tcccacgaat gaataagcaa atgcatgtga ccgattgaat ccatagtttg caaatttttc
     6181 gatatattca tatacggtcg ctgctgtttt ttccgaatat ccctgttgta atgctcctga
     6241 tacaaaatgc gacttttctt catctaatac gtcttttttc tttttactca ctgcccgtcg
     6301 cagaatatct gactgcccta atgtaaatcc agccatagtc gaggcgatct gcatgatttg
     6361 ttcctgatag acgatcactc catatgtgtt tttcaaaatc gggatcagtg aatcatctgg
     6421 gtagcgaatg gcttcttttc ctttcttcct ggcaatgaag gtatctatat tttgcatagg
     6481 gcctggtcga tacaatgcat tgactgcggc aatatcttcg atgttttctg gatctaatct
     6541 tcgtaaaacg ttccggatac cagcagattc aaattgaaat actcctgagg tttcgcctcg
     6601 ttgaaacaag agcagcgttt ttgcatcatt tagcgggatc tcttttaatc ggatctcata
     6661 cccttctact cgcttaacag ctttcaaagt aaaatcaata atcgataagt tgcgtagtcc
     6721 taaaaagtcc attttcaaaa gaccgattgc ttctacctca ttcatcgtat actgggtcag
     6781 aaaagcttca tcagaaccgc gttggagcgg aactagttca agtagattct tatcgctgat
     6841 aactacacct gctgcgtgag tcgaaacatg tcttggcaat ccttctaaaa tcactgcagt
     6901 tttgaataaa agttggtttt tttcgcttgc ctgaactaat tctactaatt gatgcgaagt
     6961 tttataagct tgggtcaatg tgattttcaa ttgattgggt attgcttttg accagcggtt
     7021 tgcttcactt tgcgataaac caaacactct agctgcatct ctaagaacca tttttgctgc
     7081 cattgtgcca aatgttgcga tttgtgccat atgatattgt ccatactttt ctcgaacata
     7141 attcaatact tcttcacgtc gattgtcagg aatatccaaa tcgatatctg gcatcgtaaa
     7201 gcgttcgggg tttaaaaagc gttcgaacag caaatcatat tctaatggat ccacatctgt
     7261 gatttctaac acataggcta ctaatgaacc cgcagcagaa ccacgacctg caccggtaac
     7321 gattttgttt ctatgagcaa aagccattac atcccatacg atcaagaaat aatcatcgaa
     7381 tcccatttga tggatgacag ataactcgta gtccaaacgt tcttggtatt cctttgtata
     7441 gtcagtgatt cggcgttcca cttgcgaata gcataactct cttaaatatt ctccagcttg
     7501 ttttccatta ggaactggat aatgcggaag taatttctga tggaactgga attcaaatga
     7561 aatcgattca gctaaacgct gggcgcctat tacagcttgg gaaagacccg ctttttcgaa
     7621 tcgtttgatc atttcttgtt ctgaaagaag atagacattt gctgttagtg gtaaagatgc
     7681 agataaattt tctaaaggta tttggcttcc ctctttgata tgtttcatca cttcaagtgc
     7741 aaaaagttct tcttgttgta aactagcgat gggatgtaat gcacaaagcg aataattttc
     7801 ttttatcaca tactgatacc attcaggcaa ttgatcaggc gtgttttctg tcaagccgat
     7861 ataaaaattt ctagtaaaaa cattttcaag taaaaactgc caatgaccga aaaagttatc
     7921 ctctttattg aaagcctcag aaacagaatg atttgccgga acaacaaacg ctagatggtc
     7981 ggtttctgtc atttctgcaa gttccaattt ttcttggatc atttttcggc tggaaagttt
     8041 catcagctgc tgataaccta tttcatcttt tgcgtacgca agcaagccat aatttttttc
     8101 ttcataagta taactaagat gtaatccaat gattggctgt actccttctt ctacacaaat
     8161 acgatagaat tcaggtgcac cgtataagac atctttatcc gttatggcta atgaagtgta
     8221 gccttgtttt tttgcttctt gtacatattc tcgaatacgt atcgtacttt gtaaaagaga
     8281 ataagaagta attgtataaa gttgtgggaa aaacatcttg cattccgcct tttttttcgt
     8341 taaaattcac tagcgttaaa aggtttgatc cttttacttt cattaagata gcaggcgctg
     8401 aagttgatta ttccacttca tttgcttgct tgtaatctgt aggaatgagt tctttcgcaa
     8461 gaactcaaat tttattgaga tttggtctcg tttctttaca ttcaaagaaa atctggtaaa
     8521 attaagatag aaaagaggtg ttctttatgc gatatatcgt tacattattc tgggctgtcg
     8581 ttctaggtca agtagtcggt tatattggtg ctgcattaac aagcggaaca tatgatttta
     8641 ctttgacaac aatcatttca ttcattgcgg gtgtcattat ccttttgatt ggtgcagttg
     8701 cgccaagaaa agaaacctct gcccattcgt aaaaaaacag ccaagagctt gagatttctc
     8761 aagctctttt ttattttcta ctcctcacca ccattataat ctgctgcgaa gaactttacg
     8821 aaagttgaat aaagaaagaa gaatatttga cagaacaaac aaacttgtca caataaaaac
     8881 agcactgtaa ttataagcac gcgcgactgt cgatcctagc atgggaccta aaacttgtcc
     8941 aaagttgcta aacatctgat tgtagctata gattcggctg actccttctt gaggcgtaat
     9001 cttactgatc agtgtattga cagaaggcat caaagcgcca gttgaaaagc ctaaaataaa
     9061 gcgaagtata cctaattgga gcggtgtagt gacgaatgcc ataggtaaat aacagcaaaa
     9121 tgataaaatg agtcctccaa ttagaacttt ttgattccca atcttatccc ctagttttcc
     9181 tagtttcggt gaagaaaaga cagcggaaac accagctatc gaaacaatca agccgctgac
     9241 aaaaaggata ttttctgtac ttccgctcaa ttcacgaata tataaggtca aaatagggct
     9301 gatcgtagtg attcctatct gtaaaatcaa ggtcgtaatg aagagaccga tcaaaataga
     9361 gagatgatcc atttttgaaa aaatttcttt ggtgcttatc agatcttttt tctcgattgg
     9421 atggaaatct tctttgacta agaaaatggt caataccgtt gtgatcatca acaaagcccc
     9481 tgtgatcaag aatacatttt ccataccaaa ccactgagcc agtgcaccgc ccattgaagg
     9541 tccgatcaga tttccagcaa tcgctccggt agacaatgtt cctaacgccc atccgctctt
     9601 ttctctaggt gcctgtgaag caatcatcgc tgtggcattt ggaatatatc cagacaaaat
     9661 cccattccaa aaccgcatga tcaaaagcca atacgcgttt ggtacaaaag ccaaagatcc
     9721 cattgttaat gtcatccctg ctgctgcacg gatcatcatg agttttcggc cttttcgatc
     9781 agctagattt ccccagattg gtgcaaccac ggcagatgca aaagcagtca cagagattgc
     9841 tagtcccgaa aacaattcga tttgatcttt tggtgttcct agttgctcaa cataaactgg
     9901 tatgaatggc ataactaaac taatacttgc accagtgaaa aaacagccaa tccacgcaat
     9961 cactaaattt tttctccaat taatctccat ctatcatccc tctttcgtct cttttatcat
    10021 acctattttc gaataaacaa gcaacgatct cgccagtaaa aagagaaaaa tcccgcaaag
    10081 gtcattttat atgtaacgtt ctttttatgt aaaacaaaaa gagatcagaa caaatactgt
    10141 tccaatccct tctctttagt taaattgttg ctagaatgat gaagttgata atgaataaaa
    10201 tatcaacgat ccacaaagca acagaaactt gattgtattt tcctttgatt actttcacaa
    10261 ttgtatagaa aataaatcct gctgcaatcc cataggaaat gctatagcac aatcccataa
    10321 agatcgatgc aaagaaggcc ggtaaagctt cttccatatc tagccatttg atgtctgcaa
    10381 aagaagccat catcattact ccaactaaaa tcaatgctgg cgcagttgct tgatttggta
    10441 caatggcgat cagtggagag aataaactac ttaatgcgaa tagcactgcc actactactg
    10501 aagtcaaacc agtacgtcca ccggccccga tcccagctgc agattcgaca taagttgttg
    10561 tattcgatgt accaaagata gcccccacag aagtagcgat cgcatcggca aatagtgctt
    10621 tatccatttt tgttttgaac ccacgaccat cttccaacgc gatctcgtct tcttttgaga
    10681 aaatacctgt gcggcgtcct gtaccgataa atgttccgat cgtatcgaaa gtatcagata
    10741 aactgaatgc gatgatcgtc atcaatactt gaggaatctt ggaagaatca ctgaataaag
    10801 attgcatgcc ttcagaacca aatgctgcac cgaaagttgt tcctaattct ttaaatgaat
    10861 tacctaacga attcgtttgc caatcaatgg aacttaaatc aacaacaccc ataaaaatac
    10921 caaggactgt ggtcgctacg ataccgataa gaatagcccc acggacattt tttacaacta
    10981 aaatggctgt cagtaccaaa ccaatcacag ccaataagat aggagcgttg tcaaagttgg
    11041 ctaacgcagg gacgattcca ccatttatcg taacatttgt cgcttttccg ccttcaacga
    11101 cagagctcga aatagctgac tggtctgctg aaaaagataa gaatcctgca tttttgattc
    11161 cgacatacgc aacgaaaata ccgatcccgc caccaatcgc atgttgaaga ctttcgggaa
    11221 tcgcccgaat aatcattttt cgaatatttg tgacagtaat aaaaatattg attaatccgc
    11281 aaataaacac cattgccaag gcttgttgcc aagaataacc aagcccaaaa acgaccgtaa
    11341 aagtgaaaaa tgcgttcaat cccataccag gtgcctgagc atatggtaca tttgcgaaca
    11401 atcccattac cagcgtacca ataatcgatg caatgattgt cgctagaaat actgcttgga
    11461 agggcatacc tgatgctgac aaaatcgtcg gatttacgaa taagatataa ctcatggcaa
    11521 agaatgtcgt tactcccgcc attatttcag ttgatacatt tgtattgttt ttctttaaac
    11581 cgaaaaactt ttccataaaa agttccactc cctgaatttt tttctgatgg aaaactataa
    11641 taataaaaaa taagtaaaat agggcaaatc catcgaacaa tttcaaatta tatagacgtt
    11701 tcattcgctt ttcaacgttt ttttaacaaa aaaagcgaaa ataatactta acgttcgtgt
    11761 ttagcttatg tttagctatt tatatagcca taactgtagt ttttgttagg aatagctgat
    11821 tgctcttgtt aattacctaa aatatgctag actaaaacaa atgctagttg aggaaggaag
    11881 gacccttatt gaataaaaaa ttaattgcca tcgatctgga cggaactacc ctcaatcaag
    11941 attcgatgat tacatctaaa acaaaagaga ctctaaaaaa agcaatcaat gccggtcatc
    12001 atgtcagtat tgctacagga cgcccattcc gaatgagcca tcagttttac caacaattag
    12061 aattaacaac accgatggtt aattttaacg gcgcattagt acacatccct aatcagcact
    12121 gggatggtga aagagaaacg ttgatcaatc gtgaaatcgc ttttgaaatc ttatcgcaaa
    12181 aaaagcagct aaatttagat tttatcgctg ctgaaaatcg tgatactttt tttattgaca
    12241 gttttgattt ctttgatgaa aaaatttttg cttcttctcg tccaggagag aagaatttgc
    12301 tttctcctaa aaatttgacg acaaatccta catctttgct tgtccgaaca gacaaacgtt
    12361 ttgcaggagc tgtatctgct gaattaacac gtcaatttgg cagttatgtc gatgttcgga
    12421 catggggtgg acctactgcc atcttagaga ttgtttcaaa aggaatccaa aaagcaaaag
    12481 gcgtgcaaga gattgctaac tacctgtcta tcgatcaaaa agacgtcatt gcctttggtg
    12541 acgagcataa tgatttggaa ttgttggatt atgcaggttg gggagtagct atggctaacg
    12601 gtactgacca actcaaaggc attgcaaatg atgtcactcc tttgagcaat caggaagacg
    12661 gtttagctgt ttatctggaa aaactactta aactataata gagaattaga ttccacaaaa
    12721 aagaggttgt ga
//
LOCUS       DACEEZ010000084        12495 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20313_2017
            SAMN12639966-rid9879223.denovo.084, whole genome shotgun sequence.
ACCESSION   DACEEZ010000084 DACEEZ010000000
VERSION     DACEEZ010000084.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639966
            Sequence Read Archive: SRR10023158
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12495)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12495)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741683.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741683.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:44:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,016
            CDSs (total)                      :: 2,962
            Genes (coding)                    :: 2,889
            CDSs (with protein)               :: 2,889
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 36 of 73
            Pseudo Genes (incomplete)         :: 29 of 73
            Pseudo Genes (internal stop)      :: 18 of 73
            Pseudo Genes (multiple problems)  :: 8 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12495
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639966-rid9879223.denovo.084"
                     /isolate="E20313_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            156..1862
                     /locus_tag="IWO89_002311"
     CDS             156..1862
                     /locus_tag="IWO89_002311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fibronectin/fibrinogen-binding protein"
                     /protein_id="HAQ0457029.1"
                     /translation="MSFDGVFTHAMVNELRETLLSGRISKIHQPYENEIVLVIRSRGK
                     NHRLLLSAHPSYARVQITQIDYQNPDNPPNFVMMLRKYLDGAILEDIEQIENDRVIHF
                     HFAKRNELGDLQNIILIVELMGRHSTIVLVNRETGKILDAIKHIGSSQNTYRSLLPGV
                     EYVAPPKQEVLNPFSSEKEKIFQRLSQTELDPKAIQRQFQGIGFDIAQELTKRLLERP
                     NEKMVVWDEFFSAISHQPIPTFYETENKDFFTPIAYQVLSEQASAVTAYPTLSQLLDS
                     YYHEKAEKDRAKQQGGELIRKIENELKRNKNKLKKREQTLKESENAENYRRNGELLTT
                     FLTQVPRGAKEVVLPNYYEEDRPIKIALDPALTPNQNAQKYFHRYQKLKNAVKLIGEQ
                     IQEAKDEIQYLESVLSQLEIAGPMDIEAIKEELTSEGYLKKKNLKKQKRKKPSQPDQY
                     FSSDGTLILVGKNNLQNDQLTMKTAKKTDYWLHAKNIPGSHVIIKSDKPSDETITEAA
                     ELAAYFSKYRYSAQVPVDLVQVKHIRKPNGAKPGYVIYENQKTIIVTPEEEKITAMKQ
                     NR"
     gene            complement(2020..2922)
                     /locus_tag="IWO89_002312"
     CDS             complement(2020..2922)
                     /locus_tag="IWO89_002312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HAQ0457030.1"
                     /translation="MNIQQMKYVVAVANNGSFREAAKKMFVSQPSLSAGIKELETELG
                     ITLFTRTNRGAYLTEEGQDFLNRAEKILVQLDTLETHYLTSEKRDNFSIAAQHYDFLG
                     PLTAQLLEEFKDEIKQFRIMETTTARVIEEVKEQHSEIGIIYMNEHNQAGIRRYLQQG
                     ELSGRSIGKFKTHIFLRQGHPLAEHAEITKEELLSYPQVRFTQDGNNFPYFYEDLIEI
                     PDQETVIYTSDRGTLMNIVLETDAYASGSGIVIGGIREHLRLIPLAEGELNEFYIIYS
                     AKRPLSEIAQRFIKELTRLLDQQS"
     gene            complement(2919..3623)
                     /locus_tag="IWO89_002313"
     CDS             complement(2919..3623)
                     /locus_tag="IWO89_002313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbonic anhydrase family protein"
                     /protein_id="HAQ0457031.1"
                     /translation="MKKNMDVEWGYSGDKGPENWASLCDWFARGAEFPLQSPIHLTKG
                     EETKIEGDTLSFHYQKQRFTDKEFKNTIHLVPFDQLSYVEFKKERYYLTDIHFHLPSE
                     HIINGKQEDIEFHFVHMNSKKENLVVGVMYQLMVQEGWLYNQENGESWNLEKHEHWVN
                     PDVFFPEQKSHFHYIGSLTTPPTSGPIQWFVMDHVGKLNQEFLLPFDGQYARPNNRPI
                     QPLNGREIYYFEEFEQ"
     gene            complement(3654..4295)
                     /locus_tag="IWO89_002314"
     CDS             complement(3654..4295)
                     /locus_tag="IWO89_002314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HAQ0457032.1"
                     /translation="MGLNEFILGLNNLETITRAPGFFKYTEHTVAAHSYRVASIAQVL
                     GDIEEANGQPIDWKLLYEKSLNHDYTERFIGDIKTPVKYASKELRGMLQTVEEKMTDE
                     FINQEIPAEFQEVYRRRLFEGKDETVEGELLAISDKVDLLYESFDEIIKNNPEKVYKE
                     MFIEAVTTIKSFEHRPSVQYFFKEIFPELLNQEFYGKEAFLETVSTIVKKEEK"
     gene            complement(4333..4602)
                     /locus_tag="IWO89_002315"
     CDS             complement(4333..4602)
                     /locus_tag="IWO89_002315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0457033.1"
                     /translation="MSPESSIGIAKIQKLIARSIFSSGMKRNFYRDQFLIFLKFIFLR
                     LVSRKKRIISSLFSIIYRFSLFFGKGAVSKMVKSGNVANISYLMI"
     gene            complement(4575..5264)
                     /locus_tag="IWO89_002316"
     CDS             complement(4575..5264)
                     /locus_tag="IWO89_002316"
                     /EC_number="2.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
                     /protein_id="HAQ0457034.1"
                     /translation="MNVEQSIAKDLLEIEAVFLNPSNPFTWASGIKSPIYCDNRITMS
                     YPKVRKEIAKGLASKIKEAYPEVQVIAGTATAGIPHAAWVAEILDLPMVYIRSKAKDH
                     GKGNQIEGRIFEGQKMVVIEDLISTGGSVLEAAEAAKREGADILGVAAIFTYELPKGK
                     ANFEKAEIPLMTLTNYSVLIEAALEDRYIDEQELTLLKEWKKDPENWQIEARLMKIEK
                     SDLCLQNHQLE"
     gene            complement(5271..5987)
                     /gene="pyrF"
                     /locus_tag="IWO89_002317"
     CDS             complement(5271..5987)
                     /gene="pyrF"
                     /locus_tag="IWO89_002317"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="HAQ0457035.1"
                     /translation="MNQLDQRPIIALDFSTRQEVEDFLRFFPKEEKLFVKIGMELFYQ
                     EGPEIVRYLKEAGHNVFLDLKLHDIPNTVEKAMRGLAKLGVDLTCVHAAGGIRMMEAA
                     MRGLEEGTPEGGKRPLLLAITQLTSTSEEEMHADQLIEVSLEKSVIHYASCAKKAGLD
                     GVVSSAWEVEAIKEMAGDEFVCLTPGIRPEGTVAGDQTRVVTPSQAKKIGSTFIVIGR
                     PITQSTNPYKAYQTIQTEWS"
     gene            complement(5984..6916)
                     /locus_tag="IWO89_002318"
     CDS             complement(5984..6916)
                     /locus_tag="IWO89_002318"
                     /EC_number="1.3.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase"
                     /protein_id="HAQ0457036.1"
                     /translation="MNENPLAVKLPGLELKNPIMPASGCFGFGKEYGKYYDLNQLGSI
                     MVKATTPNARFGNPTPRVAETPSGMLNAIGLQNPGLDVVMHKLLPELEEYNELPIIAN
                     VAGACEEDYVEVCSKIGEAPNVKAIELNISCPNVKHGGIAFGTDSDVAFQLTQAVKKV
                     SSVPVYVKLSPNVTDIVPIAQAIEAGGADGFTMINTLLGMRIDLKTRKPILANQTGGL
                     SGPAIKPVAIRLIHQVASISSLPIIGMGGVQTVDDVLEMFMAGASAVAIGTANFTDPY
                     ICPKLIKELPVRMSELGIESLERLIKEVREERER"
     gene            complement(6913..7689)
                     /locus_tag="IWO89_002319"
     CDS             complement(6913..7689)
                     /locus_tag="IWO89_002319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase electron transfer
                     subunit"
                     /protein_id="HAQ0457037.1"
                     /translation="MKQEMMEIVQQKQLAPKIFEMTLKGDLVKEMQQPGQFLHILVPR
                     ADLLLRRPISLNHIDHETNTCRIIYRVEGEGTRVFSTLSAGDHLDVMGPLGNGFDLSN
                     LHTGDEVFIIGGGIGVPPLYELSKQLVEKGIKPTHFLGFATHEVVYYEKEFNRLGETR
                     IATDDGTYGMHGNVGNLLLGTKTEPTAVFACGSNGLLKTVEQLFSSHQNVQLSLESRM
                     ACGIGACYACVCHTPEDETGTKSVKVCDEGPIFKIGEVII"
     gene            complement(7743..10928)
                     /gene="carB"
                     /locus_tag="IWO89_002320"
     CDS             complement(7743..10928)
                     /gene="carB"
                     /locus_tag="IWO89_002320"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HAQ0457038.1"
                     /translation="MPKRTDIKKIMVIGSGPIIIGQAAEFDYAGTQACLALKEEGYEV
                     VLVNSNPATIMTDKEVADQVYIEPITLEFVSRILRKERPDALLPTLGGQTGLNMAMEL
                     AASGILEELNIELLGTKLSAIDQAEDRDLFKQLMKELNQPIPESEIVNTVEEAVDFAN
                     KIGYPVIVRPAFTLGGTGGGMCENEEELRIVSENGLSLSPATQCLIERSIAGFKEIEY
                     EVMRDSADNAIVVCNMENFDPVGIHTGDSIVFAPSQTLSDHEYQMLRDASLSIIRALK
                     IEGGCNVQLALDPHSFNYYVIEVNPRVSRSSALASKATGYPIAKLAAKIALGLTLDEM
                     KNPVTGTTFAEFEPALDYVVAKIPRWPFDKFENGERLLGTQMKATGEVMAIGRNIEES
                     LLKAVRSLEIGTHHIELPELADITDDDLIQKVVRAQDDRLFYVAEAIRRGYTIEELAD
                     LTKIDLFFLDKLLHIVEIEQQLKQQPLDDKILKTAKQNGFTDKKVAELWEINEEAVRQ
                     MRKENDLIPVYKMVDTCAAEFESKTPYFYSSYEYENESSRSEKPSILVLGSGPIRIGQ
                     GVEFDYATVHSVKAIQQAGYEAIIMNSNPETVSTDFSISDKLYFEPLTFEDVMNVIEL
                     EQPIGVIVQFGGQTAINLAEPLTAAGVKILGTTIEDLDRAENRDLFEQALKELDIPQP
                     LGDTATSKEEAVVIASRIGYPVLVRPSYVLGGRAMEIVENQRDLEDYMEHAVKASPEH
                     PVLVDRYLLGGECEVDAICDGETVLIPGIMEHIERAGVHSGDSMAVYPPQTLSESIKK
                     TIEDYTIRLALGLNCVGMMNIQFVIHENEVYVIEVNPRASRTVPFLSKVTGIPMAQIA
                     TKAILGEKLKTLGYENGLYPETKEVHVKAPVFSFTKLQKVDTYLGPEMKSTGEVMGSD
                     RQLDKALYKAFEASGLHLPEYGAVLFTIADETKEEALELAKRFTEIGYSLIGTKHTAA
                     YFEKNGLVVETVAKISEEAAEKNVVDLIRDGKTQVVVNTIDKDRGNASKDGFIIRREA
                     VEHGIPLFTSLDTADAIVRVMESRAFSTNPI"
     gene            complement(10933..12009)
                     /gene="carA"
                     /locus_tag="IWO89_002321"
     CDS             complement(10933..12009)
                     /gene="carA"
                     /locus_tag="IWO89_002321"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010744830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing carbamoyl-phosphate
                     synthase small subunit"
                     /protein_id="HAQ0457039.1"
                     /translation="MERLLILEDGTVFEGKAFGADASVFGEIVFTTSMTGYQETITDQ
                     SFNGQIITFTYPMVGNYGVNRDDYESISPTCKGVVVKEHARVASNWRNQMTLDSFLKQ
                     KGIPGISGIDTRALTRKIREVGTMKASIVDAGDSFEHAFDQLKATVLATNQVQQVSTN
                     KPYPSPGTGRKVIVIDFGLKHSILRELSKRNCNLTVLPYNTDAETILALSPDGVMLTN
                     GPGDPKDVPEAIEMIKKIQGKVPIFGICLGHQLFALANGADTYKMKFGHRGLNHPVRE
                     VATGRIDFTSQNHGYAVDEATIDPEKLIVTHVEVNDGTVEGVKHRDYPAFSVQFHPDA
                     APGPHDALHLFDEFMEMMDAGKEQ"
     gene            complement(12014..>12495)
                     /locus_tag="IWO89_002322"
     CDS             complement(12014..>12495)
                     /locus_tag="IWO89_002322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HAQ0457040.1"
                     /translation="IPEDFGFWKMNPPLRGREDREALIEGLLDGTIDCIATDHAPHGL
                     EEKSQSFMKSPFGIVGSETAFQLIYTHFVETGRFTLEQVINWLAVKPAEIFGLNAGTL
                     TVGAPADVAVFDITQTCTIDKEDFLSKGENTPFIGWKVKGETQMTFVNGKLVWQKGE"
BASE COUNT         3509 a         2809 c         2221 g         3956 t
ORIGIN      
        1 acctaaaaat ttggatttca acgatagaaa cactgttttt tatgtaatta gtccaagatt
       61 tttctagact tttgctttac tattcggcca aattggtttt tcttcttttt ttcgttataa
      121 tgaatatact tatgaaaaga aatgaggaat gaaccatgtc ctttgatggt gtatttaccc
      181 acgcgatggt caacgaattg cgtgaaacgt tactatctgg ccggatctcg aaaatccatc
      241 agccttatga aaatgaaatc gtgctggtga ttcgttcacg cggcaaaaac catcgattat
      301 tattatctgc gcacccaagt tatgctcgtg tacagattac ccaaatcgat tatcagaatc
      361 ctgataatcc gcctaacttc gtgatgatgc ttcgaaaata tttggatggc gctattttag
      421 aagacatcga acaaatcgaa aatgatcgag tgatccattt ccactttgct aaacgaaacg
      481 agttgggtga tctacaaaat atcatattga tcgttgaatt aatgggacgt catagtacga
      541 tcgtcttagt taatcgagaa actggaaaaa tactggatgc gatcaaacac attggaagct
      601 ctcaaaacac ttatcgttct ttgcttcctg gtgtagaata tgttgctccg ccaaaacaag
      661 aggtcttgaa cccatttagc tccgagaaag aaaaaatctt tcagcggctt tcccaaactg
      721 aactagatcc aaaagcaatc caacgtcagt ttcagggaat cggtttcgat atcgctcaag
      781 agctgaccaa aagattactg gaacgtccta atgaaaagat ggttgtatgg gacgaatttt
      841 tctcagccat cagccatcag cctattccta ctttttacga aacggaaaat aaagattttt
      901 tcacaccgat tgcttatcag gtattgtccg aacaggcatc tgctgtaaca gcttatccaa
      961 cactcagtca attattagat agctattatc acgaaaaagc agagaaagat cgggcaaaac
     1021 aacaaggcgg agaactgatt cgaaaaatcg aaaatgaact aaaacgaaat aaaaataaat
     1081 tgaaaaaacg tgaacaaaca ctcaaagaat ccgaaaacgc tgagaattac cgtagaaatg
     1141 gcgaactact tactactttt ctgacacaag taccacgcgg cgcaaaagaa gttgttttac
     1201 ctaattatta tgaagaagat cggccaatca aaatagcact agatccggca ttgacaccaa
     1261 atcaaaatgc acagaaatat ttccatcgat accaaaagct gaagaacgct gttaagctga
     1321 ttggcgaaca aatccaagaa gccaaagatg aaatccaata tcttgaatct gtcttatctc
     1381 aactggaaat tgccggccca atggatatcg aagcaattaa agaagaattg acttcagaag
     1441 gatacctgaa aaagaagaac ttaaaaaaac aaaaacgtaa gaaaccatct cagcctgatc
     1501 aatacttttc ttctgacgga acgctcattc ttgttggtaa aaacaatctt caaaatgacc
     1561 agctgacaat gaagacagct aaaaaaaccg actattggct tcatgcaaaa aatatacccg
     1621 gttcgcacgt catcattaaa agtgacaagc cctcagatga aacaattaca gaagctgccg
     1681 agttagctgc ctatttttca aaataccgat actctgccca agtgccggtt gatcttgtac
     1741 aggtaaagca tatccgaaaa ccaaatggcg ccaagcctgg ttatgttatt tatgaaaacc
     1801 aaaagacgat tatcgtgaca ccagaagaag aaaaaattac agctatgaaa caaaacaggt
     1861 aatcaatgag tgtttctaga atcatatttg cgtactttcc cacaacagca attagttata
     1921 aattgtttgc tacaaaaaga ggagttgtga caaaattctg tcacaacccc tcttttttga
     1981 tcaacggtgt tcaataaaca gcgttctcat ctgcttttct tagctctgct ggtccagtaa
     2041 ccgagtaagc tctttgataa atctttgagc gatttcactg agcggacgtt tcgccgaata
     2101 aatgatataa aattcattta gttcgccttc tgccaaaggg attaaccgca agtgctcgcg
     2161 aatccctcca atcacaatac cagatcctga agcgtaagca tcagtttcaa gaacgatatt
     2221 catcaaagta cctcgatcac tcgtataaat cactgtttct tgatcaggga tctcgataag
     2281 gtcttcataa aaatacggga aattatttcc atcctgtgta aaacgaactt gaggataaga
     2341 taacaattct tctttggtga tctctgcgtg ttcagctaat ggatgtcctt gccttaaaaa
     2401 aatatgcgtt ttgaattttc caatgctgcg gccacttagc tctccttgtt gtaaatatct
     2461 tctgatacca gcttgattat gctcattcat ataaataatg ccgatctcac tgtgctgctc
     2521 tttgacttct tcgatcactc tcgctgttgt cgtctccatg atccgaaatt gtttgatctc
     2581 gtctttgaat tcctcgagta gctgtgcggt caaaggacct aagaaatcat aatgctgggc
     2641 agcgatcgaa aaattgtctc tcttttcact cgttagatag tgagtttcta atgtatcaag
     2701 ctggaccaat attttctcgg cacgatttaa gaagtcttgc ccttcttctg ttaaatatgc
     2761 ccctcgattc gttcgagtaa acagcgtgat ccctaactcg gtttctaatt ctttgatacc
     2821 agctgaaaga cttggctggg agacaaacat tttctttgcc gcttctctga aactaccatt
     2881 gtttgctaca gcgaccacgt atttcatctg ctgaatattc attgctcaaa ctcctcgaaa
     2941 taataaattt ctcttccatt caagggctgg attggtcggt tgttcggtcg agcatactga
     3001 ccatcgaagg ggagaagaaa ctcttggttc agttttccta catgatccat tacaaaccat
     3061 tggattggcc cgctggtagg cggagttgtc aatgagccaa tataatggaa atggcttttt
     3121 tgctccggaa agaacacatc cggatttacc caatgctcat gtttttctaa gttccaactt
     3181 tccccatttt cttgattata cagccaacct tcctgtacca ttagctggta catgacaccc
     3241 acaactagat tctctttttt gctgttcata tgaacaaaat gaaattcaat atcttcctgt
     3301 tttccgttga tgatatgttc agaaggcaag tggaaatgaa tatctgtcaa ataatagcgt
     3361 tctttcttga actctacata actgagttgg tcaaaaggta ctaaatgaat cgtgtttttg
     3421 aattccttgt cagtaaaccg ttgcttttga taatgaaagg aaagcgtgtc tccttctatt
     3481 ttggtttctt cccccttggt caaatgaata ggcgactgca atggaaattc cgcacctctc
     3541 gcaaaccaat cacataaact cgcccaattt tccggacctt tgtctccaga atagccccat
     3601 tcgacatcca tattcttttt caactatact tccccctgca tctcatatca tttttatttt
     3661 tcttctttct ttacaatcgt tgagacggtt tctaaaaacg cctcttttcc ataaaattcc
     3721 tggttcaata gttccggaaa tatctcttta aagaaatact gaacagaagg ccgatgttca
     3781 aagcttttaa tcgtagtcac tgcctcaatg aacatctcct tataaacttt ttccgggtta
     3841 tttttgatga tttcatcaaa agattcataa agaaggtcaa ccttatctga aatggctaat
     3901 agttctcctt ctacggtttc atcttttcct tcgaacaacc gacggcgata aacttcttgg
     3961 aactcagctg gaatctcttg gttgatgaat tcgtccgtca tcttttcttc aactgtttgc
     4021 agcatccctc ttaactcttt gcttgcgtat ttcaccggcg ttttgatatc accaataaaa
     4081 cgctctgtgt agtcatgatt caatgatttt tcatagagca gtttccaatc gatcggttgt
     4141 ccattcgctt cttcaatgtc tcctagaact tgcgcaatag atgctacacg atacgaatgt
     4201 gctgcaactg tatgctctgt atatttaaaa aatccaggtg cccttgtgat cgtctctaaa
     4261 ttgttcaatc ctaaaataaa ttcattcaac cccataaaag ttacctccct ttgagatttc
     4321 tttttccgaa tatcatatca ttaaataact gatattcgca acatttccgc tctttaccat
     4381 ttttgaaaca gctcccttac caaaaaatag agaaaatcta tatataatag aaaaaagaga
     4441 agagattatt ctcttctttc tgctaaccag tcttagaaaa atgaatttta aaaaaatcaa
     4501 aaactgatca cgatagaagt ttcttttcat acctgatgaa aagatgcttc tcgcaatcag
     4561 tttttggatt tttgctattc caattgatga ttctggagac acaaatcgga tttctctatc
     4621 ttcatcaagc gcgcttcaat ctgccaattt tcaggatctt ttttccattc ttttagcaga
     4681 gttaattctt gctcatcgat ataacgatct tctaaagctg cttcaatcaa tacagaataa
     4741 ttggtcaacg tcataagagg gatttcggct ttttcgaaat ttgcttttcc ttttggcaac
     4801 tcatacgtaa agatagccgc aacaccaagg atatccgcac cttctctttt tgctgcttca
     4861 gctgcttcca agacacttcc gcctgtagaa atcaaatcct cgatcacaac catcttctga
     4921 ccttcaaata tacgtccttc gatttggtta cctttcccat gatctttcgc cttacttcga
     4981 atatagacca ttggcaaatc cagtatttcc gctacccatg cggcatgagg gattcctgct
     5041 gttgcagttc cagcaatcac ttgtacttca gggtaagctt ctttgatttt gcttgctaat
     5101 ccttttgcga tctcttttcg gacttttgga tagctcatcg tgattcggtt atcacagtaa
     5161 attgggcttt tgattccact tgcccatgta aaaggattac ttggatttaa aaatactgct
     5221 tcaatttcta ataagtcttt tgcgatactt tgttcaacat tcattttagg ttagctccat
     5281 tctgtttgaa tcgtttgata tgctttataa ggattagttg actgagtgat tggtcgacct
     5341 ataacgataa aagttgagcc gatctttttt gcctgagaag gtgtgactac tcttgtctga
     5401 tctcctgcga ctgttccttc tggtcttata cccggagtta agcaaacaaa ttcgtctcct
     5461 gccatttctt tgatcgcttc tacttcccaa gcagaagaaa ccacaccatc taatcctgct
     5521 tttttggcac agctagcata atggatcaca cttttttcta aagaaacttc gattagttga
     5581 tcggcatgca tttcttcttc acttgtagaa gtaagctgag tgatagctag aagtagcgga
     5641 cgttttccac cttctggagt cccctcttct aatcctcgca ttgccgcctc catcatacga
     5701 ataccgccag cggcatgaac acaggtgaga tcaacaccaa gctttgccaa tccccgcatt
     5761 gctttttcga ccgtattcgg aatatcgtga agtttcagat ctaaaaatac attatgacca
     5821 gcctctttta gataacgtac gatttcaggt ccttcttggt agaacaattc cattccgatt
     5881 ttcacaaaca atttttcttc tttcgggaaa aatcgaagaa agtcttctac ctcttgccta
     5941 gttgaaaaat ctaatgcaat aattggtcgt tggtctagtt gattcatcgt tcacgttcct
     6001 ctctcacttc tttgattaat ctttccagcg attcgatgcc tagttcgctc attcgcacag
     6061 ggagctcttt aattagttta ggacagatat acggatcggt gaaattcgcg gtccctatag
     6121 ctacagcact tgctccagcc ataaacatct caagaacatc atccactgtc tgcacgccgc
     6181 ccatcccaat gattggaagg ctggatatac ttgctacttg gtgaatcaaa cgaatagcga
     6241 caggcttgat tgctggtccg gagagtccac ctgtttgatt ggctaaaatc ggttttcttg
     6301 tcttaaggtc aatccgcata ccaagaagag tattgatcat cgtaaaccca tctgcccctc
     6361 cagcttcgat tgcctgagcg attggaacga tgtctgttac atttggggac aatttgacat
     6421 agacaggaac agaagaaact ttctttactg cttgtgttaa ttgaaaagca acatcagaat
     6481 ctgttccaaa agcaattccg ccatgtttga catttgggca agaaatgttc agctcgattg
     6541 ctttcacatt tggtgcttcg ccaattttgc tacaaacttc tacatagtct tcttcacaag
     6601 caccagcaac atttgcgatg atgggtaact cattgtactc ctctaattct ggaagaagtt
     6661 tatgcataac aacgtctaga ccaggatttt gcaaaccaat tgcattcaac atgccagatg
     6721 gtgtttccgc cactcttggc gtgggattgc caaagcgggc atttggagtc gttgccttga
     6781 ccatgatcga tccaagctga ttcaaatcat agtattttcc gtattctttg ccaaaaccga
     6841 aacagccgct tgccggcatg ataggatttt ttaattctag gccaggaagt ttgaccgcta
     6901 aaggattctc attcatataa ttacctctcc tattttaaaa atcggtcctt cgtcacaaac
     6961 tttcacactt tttgttcctg tttcatcttc cggcgtatga caaacgcaag catagcaagc
     7021 ccctatccca catgccatgc gggattctaa tgataactgg acattttgat gcgaagaaaa
     7081 cagctgttcc actgtcttca ataacccgtt tgaaccacat gcaaaaacag ctgtaggttc
     7141 cgtcttcgtt cctaacaata aattaccgac atttccgtgc ataccgtatg ttccatcgtc
     7201 agtagcgatt cttgtttctc ctagacgatt aaattctttt tcataataaa cgacttcatg
     7261 tgtggcaaaa cctaaaaaat gcgttggctt aattcctttt tcgactaact gttttgataa
     7321 ctcatataga ggagggacac cgattcctcc accgattata aatacttcgt ctcctgtatg
     7381 aagattggac aaatcaaaac cattgcctaa aggacccatg acatctaaat ggtctccggc
     7441 agaaagagta gagaagactc tagtcccctc accttccacc cgataaatga tacggcaagt
     7501 atttgtttcg tggtcgatat gattcaagct aatcggtcgg cgtaatagca agtctgcacg
     7561 cggcacgaga atatgcaaga actgtcctgg ttgctgcatt tcttttacta gatcaccttt
     7621 caatgtcatt tcaaaaatct ttggtgccag ctgtttttgc tgaacgattt ccatcatttc
     7681 ttgtttcatc tgtcccctcc tgcaacggtt tttcgtcttt ttgtcttaac atgtgctcgc
     7741 cgttaaatag ggttagttga aaaagcacgg gattccatca ctcgaacgat cgcatctgct
     7801 gtgtctaaag aagtaaacaa tggaatacca tgttcaactg cttctcttcg gatgatgaaa
     7861 ccgtctttag aagcatttcc ccgatcttta tcaatcgtat tgacaaccac ttgtgtttta
     7921 ccatctctta ttaagtcgac tacatttttt tctgctgctt cctcagaaat tttcgctact
     7981 gtttcaacga ccagaccatt tttttcgaaa taagcagctg tatgtttcgt tcctatcaga
     8041 ctatagccaa tttctgtaaa acgtttagct aattctagtg cttcttcttt tgtttcgtcc
     8101 gcaatcgtaa ataatacggc tccatattca ggcaagtgta atccagaagc ttcgaatgcc
     8161 ttgtacaaag ctttgtctaa ttgtctgtca gatcccatta cttctccagt cgatttcatt
     8221 tcaggcccta agtaagtatc aactttctgc aatttcgtaa aagaaaagac aggtgcttta
     8281 acatgaactt cttttgtttc tggataaagt ccgttttcat aaccaagtgt ctttagtttc
     8341 tctcccaaaa tagctttcgt cgcaatttgt gccattggaa ttcctgtcac cttacttaag
     8401 aatggaacag tccgacttgc tcgaggattg acttcgatca cataaacctc attttcatgg
     8461 atgacaaact ggatattcat catcccgaca caattcaaac ctaatgctaa gcggatcgtg
     8521 taatcttcga tcgttttctt gatcgattca gataatgttt gaggtggata cacagccatt
     8581 gaatcccctg aatggacacc agcacgctca atatgctcca tgatccctgg gatgagtact
     8641 gtttcaccgt cacaaatcgc atccacttcg cattcaccac caagcaagta acggtccact
     8701 agtactggat gttctggaga tgctttcaca gcatgttcca tgtaatcttc taaatctcgc
     8761 tggttttcaa cgatttccat cgcacgtccg cctaatacat agcttggacg aacaagaact
     8821 ggataaccaa ttctcgaagc gataaccacg gcttcttctt tacttgtagc agtatcccct
     8881 aaaggttgag gaatatccaa ctctttcagc gcttgctcga ataaatcacg attttccgca
     8941 cgatccaagt cttcgatcgt tgtccctaag atttttacac cagcagcagt caaaggttcg
     9001 gctaagttga ttgctgtctg accaccaaat tgaacgatta ctcctatcgg ctgttctagc
     9061 tcgatcacat tcatcacatc ttcgaaggtc aatggctcaa aatataattt atccgaaata
     9121 gaaaagtcag tcgaaactgt ttcaggattg ctgttcatga tgattgcttc gtatcctgcc
     9181 tgctggattg ctttgacaga atgcacagtc gcatagtcaa attcaacccc ttgtccgata
     9241 cggataggac cagatcccag tactagaatt gagggctttt ctgatcgtga actttcattt
     9301 tcatattcat aggaactata aaaataaggt gtttttgatt cgaattccgc tgcacaagtg
     9361 tcaaccatct tatatacagg aatcaaatca ttttcttttc tcatctggcg aacggcttct
     9421 tcgtttattt cccacagctc cgccactttt ttgtctgtaa aaccgttttg tttcgctgtt
     9481 ttcaatatct tgtcatccaa cggctgctgt ttcaactgct gttcgatttc tacgatatgc
     9541 aacagtttat ctaaaaagaa taaatcgatt tttgtcaaat ccgctaattc ttctatggtg
     9601 taccctcttc taatagcttc agctacataa aacaggcgat catcttgtgc tcgaaccact
     9661 ttttggatta ggtcatcgtc cgtgatgtct gccaactcag gaagttcgat atggtgtgta
     9721 ccgatttcta aagaacgcac agctttcaat aaagactctt cgatattccg accgatagcc
     9781 atcacttcac cagttgcttt catctgcgtg cctaaaagcc gttctccgtt ttcaaactta
     9841 tcaaacggcc agcgaggaat tttagctaca acataatcta atgcaggttc aaattctgca
     9901 aaagtcgttc ctgtaacagg atttttcatt tcgtctaatg ttaatccaag cgctatttta
     9961 gcagctagct tagcgatcgg atagccagtc gccttacttg ctaacgcaga tgaacgagaa
    10021 acacgcggat tgacttcaat cacataataa ttaaaactat gtggatcaag agcgagttgg
    10081 acattacagc ctccctcgat cttaagtgct cgaataatcg aaagagaagc atcacgaagc
    10141 atttgatatt catggtcaga caatgtttga ctaggggcaa atacgataga atcacctgta
    10201 tggataccca ccgggtcaaa attttccata ttgcagacaa cgattgcatt atcagccgaa
    10261 tctctcatca cttcatattc aatttcttta aaacctgcaa tacttcgttc aatcaaacat
    10321 tgggtagcgg gggaaagact taatccattt tctgatacga tacgtaattc ttcttcattt
    10381 tcacacatac ctccaccagt tccgcctaaa gtaaaggctg gacgaacgat gacaggatag
    10441 ccgattttat ttgcaaaatc tacagcttct tctactgtat tgacgatttc agactcgggg
    10501 attggctgat tcaattcttt catcaactgt ttgaatagat cacgatcttc tgcttgatcg
    10561 atcgcagaaa gctttgttcc tagaagttct atgttcaatt cttctaaaat acctgatgct
    10621 gccagctcca ttgccatatt gaggcctgtc tgtccgccta aagttggaag gagagcatct
    10681 ggacgctctt ttcgaagaat ccttgataca aattcaagtg tgatcggttc gatatatact
    10741 tgatctgcca cttctttatc cgtcatgatc gtcgcaggat tagaatttac taaaaccact
    10801 tcatatcctt cttcttttaa ggcaaggcag gcttgtgttc ctgcgtagtc aaattctgcg
    10861 gcttgaccaa tgatgatcgg accagatcca atcaccataa ttttcttgat atctgtacgt
    10921 ttcggcatct gtttactgct cctttcctgc gtccatcatt tccataaatt catcaaataa
    10981 atgtagtgca tcatgcggac caggggcagc atctggatga aactgcacac tgaaagcggg
    11041 ataatctctg tgtttgactc cttctactgt tccgtcattt acttctacat gagttacgat
    11101 gagtttttct ggatcaatcg tggcttcatc gactgcatag ccatgatttt gagaagtaaa
    11161 atcaattctt ccggtcgcta cttctcgtac cgggtgattt aatcctcgat gtccgaattt
    11221 cattttatag gtatctgcac cgttggctaa agcaaataac tggtgaccga gacaaatacc
    11281 aaaaatcggt acttttcctt ggattttttt gatcatttcg attgcttctg gcacatcttt
    11341 tggatcacca ggtccattcg tcagcatgac tccgtctggt gatagcgcta aaatcgtctc
    11401 ggcatctgtg ttatagggaa gtactgtgag gttacagttt cttttactca attctcgtaa
    11461 aatactatgt ttcaagccga agtcaatgac aatgactttc cgacctgttc ctggacttgg
    11521 ataaggtttg ttagttgaga cttgctgcac ttgattggtt gctagaacgg ttgctttcag
    11581 ctgatcaaat gcatgttcga aggaatctcc agcatcaact atactagctt tcatcgttcc
    11641 gacttcacgg attttgcgag taagggcgcg cgtatcgata cctgatattc caggaattcc
    11701 tttttgtttc aaaaatgaat ctagcgtcat ttggtttcgc cagtttgaag ctacacgtgc
    11761 atgttctttt actactactc ctttgcatgt tggagaaatc gattcataat catctctatt
    11821 taccccataa ttacccacca taggataggt aaatgtaatg atttgaccgt taaaactttg
    11881 atcagtgata gtttcttgat aaccagtcat acttgttgta aaaacgattt ctccgaagac
    11941 actagcatct gcaccaaaag cctttccttc gaaaactgtt ccatcttcta atataagcaa
    12001 acgttccata cttttattct cccttctgcc acacaagttt tccgttgaca aaagtcattt
    12061 gtgtttcgcc ttttactttc cagccgataa aaggtgtatt ctcccctttt gataaaaagt
    12121 cttctttatc gatcgtgcaa gtttgtgtaa tatcaaaaac tgctacatca gcgggggcac
    12181 caaccgttag cgttcctgca ttcaaaccaa aaatctcagc cggtttaact gccaaccaat
    12241 tgatcacttg ttccaacgtg aatcgaccag tttctacaaa atgagtatag atcagttgaa
    12301 acgctgtttc gcttcctacg ataccaaatg gtgatttcat gaaactttgg cttttttctt
    12361 ctaatccatg gggggcatgg tctgtagcga tgcaatcgat cgtaccgtca agcaagcctt
    12421 cgatcaaagc ttctctgtcc tcccgtcctc ttagcggcgg gttcattttc caaaagccga
    12481 aatcttcggg aatat
//
LOCUS       DACEEZ010000085        12171 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20313_2017
            SAMN12639966-rid9879223.denovo.085, whole genome shotgun sequence.
ACCESSION   DACEEZ010000085 DACEEZ010000000
VERSION     DACEEZ010000085.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639966
            Sequence Read Archive: SRR10023158
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12171)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12171)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741683.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741683.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:44:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,016
            CDSs (total)                      :: 2,962
            Genes (coding)                    :: 2,889
            CDSs (with protein)               :: 2,889
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 36 of 73
            Pseudo Genes (incomplete)         :: 29 of 73
            Pseudo Genes (internal stop)      :: 18 of 73
            Pseudo Genes (multiple problems)  :: 8 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12171
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639966-rid9879223.denovo.085"
                     /isolate="E20313_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            592..1920
                     /locus_tag="IWO89_002323"
     CDS             592..1920
                     /locus_tag="IWO89_002323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HAQ0457041.1"
                     /translation="MKAITPDMTTKFNKQFQANIKQQALQRAVVKNGITASAENVSAH
                     VENTPVFSIDLATGKVANQMQSGRCWMFAALNTFRHKMLNQFHLKEFELSQNYTFFWD
                     KYEKANYFYENIIATGKEAIDSRKVAFLLATPQQDGGQWDMIVSLFQKYGVVPKSVMP
                     ESANSSNSRDLNNYLNKLLRKDAVVLRKMVAQGETLDAIEEKKEEMLESIYNFLAISL
                     GTPPKEFDFEYRDEEKNYHLDRGLTPQIFYDKYIGVKLDDYVSVINAPTKDKPFNRSY
                     TVEMLGNVVGGKEVKYLNVDMETFKKLAIAQLEEGESVWFGCDVGQSSTRTSGIMALD
                     AYSMDDLFDTDFTMTKAERLDFGESLMTHAMVLTGVDLVDGQSTKWKVENSWGEKVGK
                     NGFFVMSDAWMDEYTYQIVVRKDLLTKEQLNAFNEEPIVLSPWDPMGALA"
     gene            2122..2955
                     /locus_tag="IWO89_002324"
     CDS             2122..2955
                     /locus_tag="IWO89_002324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008266492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructosamine kinase family protein"
                     /protein_id="HAQ0457042.1"
                     /translation="MDIQTVLSDLKLNGKVIPVVGGDVNQTYRIKTEHRAYFLKIHPN
                     VKKGFFEAEVDGLKELSAFVRVPDTYMLGETSEGAYLLMEWIEPGKGDQRDLAAALAN
                     LHQQTAPQFGFRKDNYLGTLVQKNSFEEDWWTFFFKDRLESQISLAEETNRWNVQRQE
                     KYLRFKERVLKSVEPKKITPRLLHGDLWSGNVFFDQQGHPVFVDPAVSYGNREQDIAM
                     SQLFGGFRPEFLDAYQTIFPLEKGWKDRLPIYQLYYLLAHLNMFGESYGSQVDQLLEN
                     F"
     gene            2971..3708
                     /locus_tag="IWO89_002325"
     CDS             2971..3708
                     /locus_tag="IWO89_002325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-[acyl-carrier-protein] thioesterase"
                     /protein_id="HAQ0457043.1"
                     /translation="MAKKYTKKHEVSYYECDINQTMTFPSMLGVVIKTSEDQSDQLGR
                     GSEFVNSFGLTWVITNYSMEINRLPRVGEVISVTTQAMEYNKFFCYRNFWIHDEDGKE
                     LVKIESVFVLMDFVNRKMSSVNEEIIAPFESEKIKKIKRQEKIEKIDSGFMLPYRVRF
                     YDIDSNQHVNNAMYFNWIIDVLGYDFLTTHVPEKVIIRFDKEVEYGNEIESHFEQIPI
                     ENGVKTKHEIRMQEQLYCEANIIWKNK"
     gene            complement(3863..4831)
                     /locus_tag="IWO89_002326"
     CDS             complement(3863..4831)
                     /locus_tag="IWO89_002326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparaginase"
                     /protein_id="HAQ0457044.1"
                     /translation="MKKILVLHTGGTIAMKENMLTGGVSPDVANPLLDAEITIPKHVE
                     LLVEDIFNLPSPHIMPSHMLVLKERILSAYASGISGVVITHGTDTLEETAFFLDTTIG
                     NKLPIVLTGAMRSSNELGSDGLYNFESAIRVASCEEALDKGVLVVMNDEIHSARYVTK
                     THTTNVATFRTPTLGPIGLVTKNRILFLQELLETKRLDISAVDGTIPIVKAYAGMQGD
                     LLEAIAHTKVDGLVIEALGAGNLPPQALAALEKLLVQKIPVVLVSRCFNGIAEPVYDY
                     EGGGKELEKMGIIFCNSINSQKARLKLLIAVNYGLSGEELISFIQN"
     gene            5063..5896
                     /locus_tag="IWO89_002327"
     CDS             5063..5896
                     /locus_tag="IWO89_002327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF72 domain-containing protein"
                     /protein_id="HAQ0457045.1"
                     /translation="MIRIGLTSFKEHDKLTGKKNNTLYEYAGFLPLVELDTAYYGIPR
                     KSSVENWISEVPESFRFVVKVYQGISGQGDWKPYYQTEEEMIAHFLDVMSPMVDAGKM
                     FCFLVQFPSSFKCTKENVQYLRKLRKWFEGYPVSIELRDYSWYSDEFLERTRNFMSRA
                     AFSLAIIDEPQLLNTTIPFDPFVTNPNFALFRFHGRNAQGWQANDKEWRKKRTLYRYS
                     EKELQELAHEVKVVDSKTKEVGVIFNNNSGGDAAENALALQKILNIKPDGLNPTQMDL
                     F"
     gene            5930..6769
                     /locus_tag="IWO89_002328"
     CDS             5930..6769
                     /locus_tag="IWO89_002328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HAQ0457046.1"
                     /translation="MIKAIFFDIDGTLVTNRSKALESTKQAIEYARKNGILCGVATGR
                     SPVKIKEIIDELELDMYVVYNGQLVFTADRTIIDHPFEQKVLEHIVEFADENHRQIVF
                     GARNRLDGSTTMLLGQSIFIKRLVSFLPRKFPVRLMKKILQVFSPHRQKDRYETLEIL
                     KEPIYQCILLSPESEQKKLEHRFPECTFQRSNTYTVDIIPKGGSKLLGIQAFANAVEI
                     EMEEIMAFGDHYNDIEMLKGVGIGVAMGNAQIEVKHAADFVTQSNEEDGIYEGLKHFG
                     LIY"
     gene            6795..7355
                     /locus_tag="IWO89_002329"
     CDS             6795..7355
                     /locus_tag="IWO89_002329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HAQ0457047.1"
                     /translation="MEAETPFQKVETFHKTFDPRRPEIPTAFTENEAHTRAAFKIEEL
                     VEFVAATSPNKEQLHEAVMLLHEALDKAEEKILGKTQWGSDPLVEQVDALTDILYFTY
                     GSFSLLGIVPDRIFEIVHQANMGKLFPDGKPHYDPVTNKVLKPENWQMEYAPEPKIAA
                     EIQRQIQEAIAKKKSADIGMCTNEKE"
     gene            7554..9275
                     /locus_tag="IWO89_002330"
     CDS             7554..9275
                     /locus_tag="IWO89_002330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septation ring formation regulator EzrA"
                     /protein_id="HAQ0457048.1"
                     /translation="MGNNVIIGIVIAIIIVAIVVFLVSYLMKKKNQDRLDALEKRKEE
                     LFDLPVIEEVDDVKKMHLVGQSQNTFREWSQKWNDISTSSFAELESQIFEVENLNESF
                     RFLKGKKAVVEAEATMDQMEKQVTEIREGLKELRETEERNSLAVQQALDVYEELKEKV
                     REDEHEYGPALFELQKQIKVIEKEFTQFVTLNTSGDPVEAREVLDKAEKHTYEVEALM
                     KKIPPLYEELHTTFPEQLEEISDTYEKLMEEQYVFPEEDLAEDIAKVSRRIENSLANL
                     EKTEVETVEFENRETADLIDSLYDILEREMEAQRYVKTNQSTIAEYIKHTTKNNRQLL
                     IELDHTSQSYTLNHNEIGRVRGFQTEVEEMERQNEQMIPQIRQHEIPYSEVRTFYKTV
                     FKVLEDIEKQQVEIDDSLHELRKGEKEAQEKIDTFEFKLRSLKRFVEKQRLPGLPNDY
                     LEFFFVATDRIEELSVVLNKIRVNMEEVNRLVALCEEDLELLDKKTHDLVDAAALTEQ
                     MLQYANRYRHTHEEVREAIDKSYYLFNKEYHYQEALDEIGTALERVEPGAFKRIEDFY
                     FNHPDLV"
     gene            9440..10582
                     /locus_tag="IWO89_002331"
     CDS             9440..10582
                     /locus_tag="IWO89_002331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HAQ0457049.1"
                     /translation="MIYFDNSATTPIFPQSLDAYVKTSQRIIGNPSSLHDLGNQANRL
                     LQQARKQIADLIHVEPEEIYFTSGGTEGDNWVLKGTMIEKREYGNHMIISGVEHPAVS
                     ETAEQLKELGIEVSIAPVDKRGFVRVDELKELIRKETVLVSVMAVNNEVGSIQPIQEI
                     SDLLDDFPKIHFHVDAVQAIGKVPFSQWLTERVDFATFSAHKFHGPRGTGFIYWKKGR
                     RLAPLLTGGGQERNQRSGTENVPGIVAMVKALRLSLEIKEQKPEQTQRLKVALINALK
                     TYEKVTIFSEGTDYAPHILCFALKGIRGEVLVHALEEKQIFVSTTSACSSRKHMASST
                     LHAMHVPNDLATSAVRISLDENNSLAEVEQFMIIFKQLYKKFSAIN"
     gene            10598..11809
                     /gene="thiI"
                     /locus_tag="IWO89_002332"
     CDS             10598..11809
                     /gene="thiI"
                     /locus_tag="IWO89_002332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 4-thiouridine(8) synthase ThiI"
                     /protein_id="HAQ0457050.1"
                     /translation="MKYSEIMVRYGELSTKGKNRKSFIMQLAQNVRQALKDFPEIKIH
                     ADRDRMHLLLNGADGQLVIPKLAKIFGIQNFSPSIRVEKDVEVLKQAVQDIMKEIYTG
                     KETFKIIAKRSDHQFELTSNELNQTLGNAVFDIFPHIQVQMRQPDIPLRVEIRRDGAY
                     LSYETIKGAGGLPVGTSGKGMLMLSGGIDSPVAGYFAMKRGVEIEAVHFASPPYTSEQ
                     ALQKAKDLTAKLAPYVGTIHFIEVPFTEIQEEIKKSVPQGYWMTITRRMMLRLTDEIR
                     RIRHGLVILNGESLGQVASQTLQSMVAINEVTNTPIIRPVATMDKLEIIEVAEQIDTF
                     NLSIQPFEDCCTIFAPPQPKTRPQLEKVHQYEERLAIDEMIQRALAGLKMEEIQAESS
                     NEKTEEFSELL"
BASE COUNT         4148 a         2042 c         2527 g         3454 t
ORIGIN      
        1 aaagcttatg caaagaaaaa gaaatccaat cacttgggtt tctttttctt ttattattat
       61 gatgaatcaa gctttctgtt tttgtcacgt atgttgtaat ctttacgaaa gagtagagtt
      121 ggctactcat aatcaaccat acttcccatc atcgctaaat acgaaataaa tcagcattcc
      181 tacaccaatc aaagcacaag ctgcaccaag aaaagggatg acattcccaa agttatctgt
      241 attcataagt atgccaccac ccacaaacaa aaagccgcca gcaatcaggt ttttcatttt
      301 attcatctcc tttcttacgt tatagtattt ccttctttca atgagtattt tgaaagctat
      361 cttattgtat gatgttcttc taggcctctt ctttattata caggccattt tcgttatctc
      421 atagaaatat tcctttttat ctgaaaacta ttgtataccg tatgacattt ttcttcatga
      481 agttgtaagt atattctcat tcaaatgaaa atttcatcat agaagagatt gtatttctaa
      541 attcttaaga gtaaaatacg aagtataact tggatttgga aggatgattg gatgaaagct
      601 attacgcctg atatgacaac aaaatttaac aaacagtttc aagcaaatat caaacagcag
      661 gcattgcagc gggcagttgt gaagaacgga attactgctt ctgctgaaaa tgtttcagca
      721 catgtggaga atacaccggt attttctata gatttggcta cggggaaagt ggctaatcaa
      781 atgcaatcgg ggcgttgctg gatgtttgct gcactgaaca cattccgtca taagatgctg
      841 aatcaattcc atttaaaaga gtttgaatta tcacaaaatt acactttttt ctgggataaa
      901 tatgaaaagg caaattattt ctatgaaaac atcattgcaa caggaaaaga ggcaatcgat
      961 agccggaaag tagctttctt actcgctact cctcaacaag atggcggcca atgggatatg
     1021 atcgtttcat tattccaaaa atatggcgtt gtgcctaaat cggtgatgcc ggaaagtgcg
     1081 aatagttcaa attctcgaga tttgaataac tacttgaaca aattactgcg taaagatgca
     1141 gtagtgttac ggaaaatggt tgctcaagga gaaacattag acgctattga agaaaagaaa
     1201 gaagaaatgc tagaaagcat ttataacttc ttagctatct cattaggaac acctccaaaa
     1261 gaattcgatt ttgagtaccg cgatgaagaa aagaactatc atcttgaccg aggactgaca
     1321 ccacaaattt tttatgataa atacatcggt gtaaaattag atgattatgt tagcgtgatc
     1381 aatgcaccaa caaaagacaa accattcaat cgttcttata cagtagagat gctaggaaat
     1441 gtcgttggcg ggaaagaagt caaatatctg aatgttgata tggaaacttt caaaaaatta
     1501 gcgattgctc agcttgaaga aggagaatct gtttggtttg gttgtgatgt aggtcaatct
     1561 tctactagaa ctagtggcat catggcctta gatgcttatt caatggatga tctatttgat
     1621 acggatttta caatgactaa agctgaacgt ttagattttg gagaaagtct gatgacccat
     1681 gcgatggtat tgaccggtgt tgacttagta gacgggcaat caacaaaatg gaaagtcgaa
     1741 aatagctggg gcgaaaaagt tggaaagaat ggcttctttg ttatgagtga tgcctggatg
     1801 gacgaatata cgtaccaaat cgttgtaaga aaagatttat tgacgaaaga gcaattaaat
     1861 gcgttcaatg aagaaccaat tgttctatct ccgtgggatc caatgggtgc tttagcatag
     1921 acgagtattt caatgaagag tatgtagtaa agataaaaag agctggctta agccagctct
     1981 ttttatcttt aaagtccatt ccctgttttt tatggtccct gtgctataat tctaaataaa
     2041 ggataaggat tggaaataat ccttttatca ttaaaaatat gatgacgaag tagaataaaa
     2101 ttgggagtag gggggaaagt catggatatc caaactgttt tatctgatct gaaattaaat
     2161 ggtaaagtga ttccagttgt tggtggcgat gttaatcaga cctatcgtat aaaaaccgaa
     2221 catcgtgctt attttttaaa aatccatccg aatgtaaaaa aaggattttt tgaagcagaa
     2281 gtagacggat taaaagaact gtcagcattc gtacgtgtac cggatacgta tatgctaggt
     2341 gaaacaagtg aaggcgctta tttgttaatg gaatggatcg agccgggaaa aggggatcaa
     2401 cgagatcttg ctgcagcttt agctaatctc catcagcaaa cagcgccaca atttggtttt
     2461 cgtaaagata actatttggg tacacttgtt cagaaaaatt cttttgaaga agattggtgg
     2521 acttttttct ttaaagatcg gcttgaatcc cagatttctt tggcagaaga aacgaatcga
     2581 tggaatgtac agcgtcaaga aaagtatctg cgatttaaag aacgtgtatt gaaatcggtt
     2641 gaaccaaaaa agatcactcc acgcctcttg cacggagacc tgtggagtgg taatgtgttc
     2701 tttgaccagc aagggcatcc agtttttgtc gacccagctg tttcttatgg gaatcgggaa
     2761 caagatattg caatgagcca gttattcggt ggtttccgac cggaattttt agatgcttat
     2821 caaactatat ttccattaga aaagggatgg aaagaccgac tgccgattta ccagttatat
     2881 tatcttttgg cacatttgaa tatgtttgga gaaagttacg gttcccaagt agatcaatta
     2941 ctagaaaatt tttaagagga gggcgtgttt ttggcaaaaa aatataccaa aaagcatgaa
     3001 gtttcatatt atgagtgtga catcaaccaa acgatgacat ttccttcgat gttaggagta
     3061 gttatcaaaa cgtctgaaga tcaaagcgac cagttgggaa gaggcagtga attcgtcaat
     3121 agctttggac ttacttgggt catcacgaat tattccatgg agattaatcg tctccctaga
     3181 gtcggtgaag taatctcagt aacgactcaa gccatggagt acaataagtt tttctgttat
     3241 cggaacttct ggatccatga tgaagatggg aaagaattag tcaaaataga atctgttttc
     3301 gtattgatgg attttgtgaa tcgaaaaatg agcagtgtta atgaagagat cattgctcct
     3361 tttgagagtg agaagatcaa gaaaatcaag cgtcaggaaa aaattgaaaa gattgattcg
     3421 ggcttcatgt tgccatatcg tgttcgtttt tatgatatcg atagtaatca gcatgttaat
     3481 aatgcgatgt attttaattg gatcattgac gtattaggat atgacttttt gactacgcat
     3541 gttccagaaa aagtaattat tcgatttgat aaagaagtag agtacgggaa tgagatcgaa
     3601 agccattttg aacaaatccc tatcgaaaat ggtgtcaaaa caaaacatga aatccgaatg
     3661 caagaacagc tgtattgtga agcaaatatt atatggaaaa ataaataaaa aagaggttgt
     3721 gacagaaggg ggcaacttca agaaataaga agaaatttac gaaaattgtt tctcaaattt
     3781 ttgtaaattt tcgacttagt tttcgaagaa gctatttttg tcacagcctc tctttttaag
     3841 tttagctatt agataatgat gtttagtttt ggataaacga aatcaattct tcacccgaca
     3901 aaccatagtt tacagcaatc aatagtttta aacgtgcttt ttgactatta atactgttac
     3961 agaaaataat ccccattttt tctagttctt ttcccccgcc ttcataatca taaacaggct
     4021 ctgcgattcc attgaaacaa cgagaaacaa gtactacagg gattttttgg acaagaagct
     4081 tttccaaagc agctaaggct tgtggaggga gattccccgc acctaaagct tcgattacca
     4141 aaccgtctac tttcgtatgg gcgatggctt ccaataagtc cccttgcatc ccagcatatg
     4201 ctttaacgat cgggatagtc ccatctacag cggaaatgtc cagtcgcttt gtttctaaaa
     4261 gttcctgcaa gaataaaatt cgattcttag ttactaagcc gatgggaccc aatgtaggtg
     4321 ttcggaaagt agcaacattt gtggtatggg tctttgtcac gtagcgtgca gaatggatct
     4381 catcgttcat gaccaccaaa acccccttat ctagcgcttc ttcacaactt gcgacacgaa
     4441 tcgcactttc aaaattataa aggccgtcac ttccaagttc attacttgaa cgcattgccc
     4501 cagtcaaaac aattggtaat ttgttgccaa tcgttgtatc aagaaaaaaa gcagtttctt
     4561 ctaatgtatc tgtaccgtgt gtgatcacca ctccggatat acctgatgca taagcggaca
     4621 agatacgttc ttttaatacc aacatgtgag aaggcatgat gtgtggtgag ggaagattga
     4681 agatatcttc aactaataat tctacatgct tggggattgt gatttctgca tctaataatg
     4741 ggtttgccac atcaggacta acaccgcccg tgagcatgtt ttctttcatg gcgatcgttc
     4801 cgccggtgtg tagtacaagt atttttttca ttttacagac ttccttacga ataattttgc
     4861 atagccgtta aatcttattg tatactatat aaaagagttt tgctatcagt gaagaataac
     4921 gaatagccta acttatcata aaaaagctga ccttattaaa atggaaaaga ctcaaccttg
     4981 acctcctaat acaatggagt atctggttga gtttctttta caagaaagca gccaaggaaa
     5041 tcaatcacga aggtggccgg aaatgatcag aatcggatta acatcgttta aagaacatga
     5101 caaattaact ggtaaaaaga ataacactct ttatgaatat gcagggtttc tacccttagt
     5161 ggaattagat actgcctatt atggaattcc ccgaaagtcc tcagttgaga attggataag
     5221 tgaagtacct gaatcatttc gctttgtagt caaagtctat caaggaatca gcggtcaagg
     5281 agactggaag ccttattatc agaccgaaga agagatgatt gcgcattttt tagatgttat
     5341 gtcgccaatg gtagatgctg gaaagatgtt ttgcttttta gtccaattcc cctcatcatt
     5401 caaatgtacg aaagaaaatg tccagtactt gcgcaaactt agaaagtggt ttgagggtta
     5461 tcctgtttct atagaattgc gggattattc ttggtacagc gatgaatttc ttgaacgtac
     5521 aagaaacttt atgtctagag ccgctttttc tttagccatc atcgatgagc cacagttgct
     5581 gaatacaacg attccttttg atccatttgt caccaatccg aactttgctc tttttcgatt
     5641 ccatggtagg aatgcgcaag gatggcaagc aaatgataaa gagtggcgta aaaaaagaac
     5701 gttatatcgt tattcagaaa aagaactgca agaactagca catgaagtca aagtagttga
     5761 ttctaagaca aaagaggtag gtgtgatttt taataataat tctggtggtg atgcggctga
     5821 aaatgcatta gcactgcaaa aaatattaaa tatcaagcca gatggactca atccaaccca
     5881 aatggatctg ttttaagggc ttattttatt caagaaaggg gcgaagtcta tgatcaaagc
     5941 tatttttttt gatattgacg gaacattggt caccaatcgt tcaaaagcgt tggaatcaac
     6001 aaaacaagca attgaatatg ctcggaaaaa cggcatttta tgcggtgtag caacggggcg
     6061 cagcccagta aagatcaaag aaatcattga tgaattggaa ttagatatgt atgtcgtgta
     6121 taatggtcaa ctggttttta ctgcagatcg cacgattatc gaccaccctt ttgaacaaaa
     6181 agtattggaa catattgttg aatttgcaga tgagaaccat cgacaaatcg tttttggtgc
     6241 gagaaatcgg ttagatggat caacgacgat gctattagga caatcgattt ttatcaagcg
     6301 actagtcagc tttttaccgc gtaaattccc ggtacgtctg atgaaaaaaa tcctccaggt
     6361 ttttagccct caccgacaaa aggatcgtta tgaaacgtta gagatattaa aagaaccaat
     6421 ttatcaatgt atattgctca gtccggagtc agaacagaaa aagttagaac atcgctttcc
     6481 cgagtgtacg tttcagcgtt caaatacgta tactgtagat atcatcccaa aaggtggttc
     6541 aaaattacta ggcatccaag ctttcgcaaa tgctgtcgaa attgaaatgg aagaaattat
     6601 ggcattcggc gatcattaca atgatatcga gatgctgaaa ggtgtaggaa ttggagtggc
     6661 catggggaat gctcagatcg aagtaaaaca tgctgcagac tttgtgactc aatcaaatga
     6721 agaagatggg atatacgaag gactgaaaca cttcggtctg atttattagt caatgaaaga
     6781 aggaaaaaat cagtatggaa gcagagacac cttttcaaaa agtagaaaca ttccacaaaa
     6841 cttttgatcc aagaagacca gagataccca cggcttttac agaaaatgaa gctcatacac
     6901 gtgctgcgtt caaaatagaa gaacttgttg aatttgttgc agctacttca cctaataaag
     6961 aacaactgca tgaagctgtt atgctgctgc acgaagcatt agataaagca gaagaaaaaa
     7021 tacttggtaa aacacagtgg ggaagtgatc cgctggttga acaagtagat gcactgacag
     7081 atatccttta ctttacttat ggttcttttt cattactagg aatagtgcct gatcgaatat
     7141 tcgaaatcgt ccatcaggca aatatgggga aattatttcc agatggaaaa cctcattatg
     7201 accctgtaac aaataaagtg ttgaaacctg aaaactggca gatggaatat gctccagaac
     7261 ctaaaatcgc ggcagaaatc caacgtcaga ttcaagaagc gattgctaag aagaaatccg
     7321 ctgatatagg catgtgcaca aatgaaaaag agtgatcaga gtttcactat ttttgttatc
     7381 tttatacgat ggaaaaacaa aagattttcg tttaagttag tgaatgcatg aaaatagtat
     7441 gctataatct agaatagatg tcttttgcac gagagacgta ttatttggat ttatgccaag
     7501 cagaacgcca tctgttgcat aaaagatgca tactgaaaac ggaggaacca taaatgggaa
     7561 ataatgttat catcggaatc gtcattgcaa tcattatagt cgccattgtc gtctttttgg
     7621 ttagttactt gatgaagaaa aaaaaccaag atcgattgga tgccttagaa aaacgcaagg
     7681 aagaattatt tgacttgcca gtgattgaag aagtcgatga tgttaaaaaa atgcatctgg
     7741 ttgggcaaag ccaaaatacc tttcgtgaat ggagtcaaaa atggaatgac atatctactt
     7801 cctcgttcgc tgaactagaa agtcaaattt tcgaagtgga aaacctcaat gaaagttttc
     7861 ggttcttaaa aggaaaaaaa gcagttgttg aagcagaagc tacaatggac caaatggaga
     7921 aacaagtgac ggaaatccgt gaaggtctaa aggaattgcg ggaaacggaa gaacgcaact
     7981 cactagctgt tcagcaggca cttgacgtct atgaagaact gaaagaaaaa gtcagagaag
     8041 acgagcatga atacggacca gctctttttg aattgcaaaa acagatcaaa gtgatcgaaa
     8101 aagaatttac gcagtttgtg actttaaaca cttctggaga ccctgttgaa gcccgcgagg
     8161 ttttggataa agcagaaaaa catacgtatg aagtggaagc tctgatgaaa aaaattccac
     8221 cgctgtatga ggagctccat accactttcc ctgaacaact tgaagaaatc agtgatactt
     8281 acgagaaatt gatggaagaa caatatgtat tcccagaaga agatcttgct gaagatatcg
     8341 caaaagtaag tcgaagaatt gaaaattcat tagccaatct tgaaaaaaca gaagttgaaa
     8401 cggtcgaatt tgaaaaccgt gaaacagctg atttgattga ttcgttatat gatatcttag
     8461 aacgggaaat ggaagctcag agatatgtaa aaactaacca atcaacgatt gcagagtata
     8521 tcaaacatac aacaaagaat aaccgtcaat tattgattga gctggatcac acatctcaaa
     8581 gctatacatt gaaccacaat gaaattggcc gtgtacgagg tttccaaacc gaagtggaag
     8641 aaatggaacg tcaaaatgaa cagatgattc cacagatccg tcaacacgaa attccttatt
     8701 cagaagtgcg cacgttctat aaaacagtct tcaaagtctt agaagatatc gaaaagcaac
     8761 aagtagagat cgacgactca ttacatgaat tgagaaaagg cgaaaaagaa gcgcaagaaa
     8821 agatcgatac attcgaattc aaattgcgca gtttgaaacg ttttgtagaa aaacaacgtt
     8881 tgcctggctt gccgaatgat tacctagaat tcttttttgt tgctactgac cgtattgaag
     8941 aactaagtgt tgtcttgaat aaaattcgag taaacatgga agaggtcaat cgtttagttg
     9001 ctctatgtga agaagatcta gaattattag ataagaagac acatgattta gtagatgcag
     9061 cagcattaac ggagcaaatg ctccaatatg caaatcgtta tcgtcataca cacgaagaag
     9121 tgagagaagc aattgataaa agctactatt tattcaataa agaatatcac tatcaagaag
     9181 cattagacga aatcggtaca gcattagagc gagtagaacc tggtgctttc aaacgcatcg
     9241 aagattttta ttttaatcat ccagacttag tataaagcaa aaaagttagt aaaccagcaa
     9301 gagcaagacg gattattgtc ttgttcttgt ctttttttgt atatttgaaa attgaaacaa
     9361 gtgaaaattt cacctgctct tgaaagcacc ttggacaacc gttataatag aaaagaattt
     9421 caaaaaagag gggtttctta tgatatattt tgataacagt gcaacaaccc caatctttcc
     9481 gcaaagctta gatgcatatg taaaaaccag tcagcggatt attgggaatc catcaagctt
     9541 gcatgattta ggcaatcaag ctaaccggtt gttgcagcaa gcaagaaagc agatcgctga
     9601 tttgatccat gtagaaccag aagaaatcta ttttacttca ggcggaacag aaggagataa
     9661 ctgggtatta aaaggaacga tgatcgaaaa aagagaatat gggaatcata tgatcatttc
     9721 aggagtcgaa catccggccg tcagcgagac agcagaacaa ttaaaagaat taggaatcga
     9781 agtaagtatc gccccagtgg ataaacgcgg atttgtacgt gtcgacgaat tgaaagaatt
     9841 aataagaaaa gagacagtat tagtctctgt tatggctgtg aataatgaag taggatcgat
     9901 tcagcccatc caggaaatca gtgatctttt ggatgatttt cctaaaattc atttccatgt
     9961 agatgctgtt caggcgattg gaaaagtacc attctcgcaa tggttgacag aaagagttga
    10021 ttttgcaaca ttttcagctc ataaattcca tggcccacgg ggaactggtt tcatttattg
    10081 gaaaaagggt cgtcgtttag caccattact gacaggtgga gggcaagaaa gaaatcagcg
    10141 aagtgggaca gaaaatgttc caggtattgt ggctatggtt aaagctttac gtttatcatt
    10201 agagataaag gaacaaaaac ctgagcagac acaacgctta aaagtagctc taatcaatgc
    10261 gttgaagacg tatgagaaag tgacgatttt ttcagaagga actgactatg caccgcatat
    10321 tttatgtttt gctctaaaag gaattcgcgg cgaagttctc gtccatgcct tagaagaaaa
    10381 acaaatattt gtttctacta ctagtgcctg ctcctcacgt aaacatatgg caagcagtac
    10441 attgcacgca atgcatgtac caaatgatct ggctacatct gctgttcgaa tcagcttaga
    10501 tgaaaacaat tcgcttgcag aagtcgagca atttatgatt attttcaagc agctttataa
    10561 gaaattttct gcaatcaatt aaaagaggag tgttcttgtg aaatacagtg aaattatggt
    10621 ccgttatggg gaactttcga caaaaggaaa gaatcgaaaa agctttatca tgcaactagc
    10681 acaaaatgtc agacaagctt tgaaagactt tccagaaatc aaaatccatg ctgatcggga
    10741 ccggatgcat cttttattga atggcgcaga cgggcagcta gttattccaa aactagcaaa
    10801 aatatttggg atccaaaatt tctctccaag tattcgtgta gaaaaagatg tcgaagtttt
    10861 gaaacaagct gttcaagaca tcatgaaaga aatttatact gggaaagaaa catttaaaat
    10921 tatcgctaaa agaagcgatc accaatttga attgacaagt aatgaattga atcaaacatt
    10981 aggtaatgct gtttttgata ttttcccgca tatccaagtt caaatgcgtc aaccagatat
    11041 tccattacgt gtagagattc ggcgtgatgg cgcttatcta tcgtatgaaa cgatcaaggg
    11101 agctggagga ttgcccgtag gaacaagcgg aaaaggaatg cttatgcttt ctggggggat
    11161 tgattctcct gtagcagggt actttgccat gaagcgggga gtggaaattg aagctgttca
    11221 ttttgctagt ccgccgtata caagtgaaca agcactgcaa aaagcaaaag atctaacagc
    11281 caaactagca ccttatgtgg gaacgatcca ttttatcgaa gtgccattta ccgagattca
    11341 agaagaaatc aaaaagtcag tgccacaagg atattggatg acaattactc gtcgaatgat
    11401 gttgcgtttg accgatgaaa ttcgccgtat tcgtcatggt ttagtcattt tgaatggtga
    11461 atcattaggt caagtggctt cacaaacttt gcaaagtatg gtggctatca atgaagtaac
    11521 gaatacgcca atcattcggc ctgtagcgac aatggataaa cttgaaatca ttgaagtagc
    11581 agaacagatc gatacattta atctttctat ccagcctttt gaagattgct gtacgatttt
    11641 tgctcctcca cagccaaaaa cacgccctca actggaaaag gtccatcaat atgaagagcg
    11701 tttagcaata gatgaaatga tccaacgagc actagctggc ttgaagatgg aagagataca
    11761 agcagaatcg tctaatgaaa aaactgaaga attttcagaa ttgctataag aggttatgaa
    11821 aatagtgttt tgaccagagt attagagaaa caaaacgaaa aaatagctcc atgtatttta
    11881 ccgtttttga actaatgtcg aagattagct gattgaatac catttatcaa gaagttatgg
    11941 aaaagccggt tcttttttac taactgaaaa ataaaatttt atttagagat caagctacag
    12001 ttgataaatg atataaaaaa gagaatttcc catgaaatag cgaggttgtg acagaagtgg
    12061 gcagcttcaa gaaataagaa ggaatttacg aaaattgctt ttcaaatttt tgtgaaattt
    12121 cggcttattt ttcgaagaag ttacttctgg aacaccgtgt atcagaggtt g
//
LOCUS       DACEEZ010000086        11667 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20313_2017
            SAMN12639966-rid9879223.denovo.086, whole genome shotgun sequence.
ACCESSION   DACEEZ010000086 DACEEZ010000000
VERSION     DACEEZ010000086.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639966
            Sequence Read Archive: SRR10023158
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11667)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11667)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741683.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741683.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:44:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,016
            CDSs (total)                      :: 2,962
            Genes (coding)                    :: 2,889
            CDSs (with protein)               :: 2,889
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 36 of 73
            Pseudo Genes (incomplete)         :: 29 of 73
            Pseudo Genes (internal stop)      :: 18 of 73
            Pseudo Genes (multiple problems)  :: 8 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11667
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639966-rid9879223.denovo.086"
                     /isolate="E20313_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(37..216)
                     /locus_tag="IWO89_002333"
     CDS             complement(37..216)
                     /locus_tag="IWO89_002333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0457051.1"
                     /translation="MPIAIGNKRLPVTLDEKRQKELQQLKQKYGKSESRIMCIALDLL
                     IAQEKAGFEVPALKK"
     gene            complement(597..1304)
                     /locus_tag="IWO89_002334"
     CDS             complement(597..1304)
                     /locus_tag="IWO89_002334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0457052.1"
                     /translation="MNKVVYQLTTEDVSQFLTNKEHLNEVIRRIQKDFSCMEEMKYFI
                     QRVEEQLYMESETSQTKVTVSHNRLDVYKRRPDTLKKGAELLRLETEKYIMNVYLERS
                     ELVENFVSYTICRKHYYSESEYYSIFESMELMEEEMEELNSKDATVDDYYLVFTRFLT
                     CGTESIVLMGMDYELTVPEAFLREYVEEVDNPGDDWYTTLTDFLDTYTYDDTEEIQNE
                     LDRKRMPYQRFERKKGQ"
     gene            complement(1297..1734)
                     /locus_tag="IWO89_002335"
     CDS             complement(1297..1734)
                     /locus_tag="IWO89_002335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0457053.1"
                     /translation="MAQNYYDEFVKLPLDKMAQKMEDMTFLYHETRVPKKHYKEKLSV
                     AVEEMIESGVEMNLIATYYRTLEELKKQNGKWFFQALLCLEVGVKPSTIKPSEYQALE
                     LTYAKFAQIKKAKAMASDWLDTFENIEKNGAFYTIESEEKDNE"
     gene            complement(1749..2000)
                     /locus_tag="IWO89_002336"
     CDS             complement(1749..2000)
                     /locus_tag="IWO89_002336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0457054.1"
                     /translation="MKTQHKLLTVKEVVQILVDKEVISEKVAEALLIPRYWTIETPLV
                     EDAPTMLFEDLVKIAGYELHYQENMETYEIEYWLTKLEN"
     gene            complement(2000..2206)
                     /locus_tag="IWO89_002337"
     CDS             complement(2000..2206)
                     /locus_tag="IWO89_002337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase UvrA"
                     /protein_id="HAQ0457055.1"
                     /translation="MRKKFDYWGVPFSELFPNYHAPHTVECDCGERAKCIKSYRLYQC
                     PTCGKKYTLSYGDYVLIDEKVKKR"
     gene            complement(2643..2903)
                     /locus_tag="IWO89_002338"
     CDS             complement(2643..2903)
                     /locus_tag="IWO89_002338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0457056.1"
                     /translation="MNKYVGLLDKIRVIKTQPLLVRFTLQTIHESINCVVADIEIIDK
                     LLIMDDGKYNIAVTGHFNKRNQLVIESMQIRNPDHFTRSMGI"
     gene            2978..3181
                     /locus_tag="IWO89_002339"
     CDS             2978..3181
                     /locus_tag="IWO89_002339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative holin-like toxin"
                     /protein_id="HAQ0457057.1"
                     /translation="MIKTVKFVTVAIRLKVRWCVCAYFQNLTERRSLLPVETALGLMI
                     SFGSLIATIIFGILAVVNNNQKK"
     gene            complement(3322..3807)
                     /gene="ssb"
                     /locus_tag="IWO89_002340"
     CDS             complement(3322..3807)
                     /gene="ssb"
                     /locus_tag="IWO89_002340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016186236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HAQ0457058.1"
                     /translation="MINNVTLVGRLTKDPDLRYTASGTAVATFTLAVNRNFTNQNGNR
                     EADFINCVIWRKPAETMATLAKKGSLIGVVGRIQTRTYDNQQGQRVYVTEVVADNFQL
                     LESKAATESRAHADQSSSSPSPTTFEQRDVATPNNNGLNASQNPFGGQSIDISDDDLP
                     F"
     gene            complement(3810..4709)
                     /locus_tag="IWO89_002341"
     CDS             complement(3810..4709)
                     /locus_tag="IWO89_002341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyl transferase AbiEii/AbiGii toxin
                     family protein"
                     /protein_id="HAQ0457059.1"
                     /translation="MTPTQFAAKTRNLAKSTGLNAQLVQRHFMMDRLIEQISESKYKN
                     DFILKGGFLLGSKYGIDRRTTIDIDTTFRNSKLTEEKLKSIFNDLTRKPTKEGIVFSI
                     QGMKETREADFYPGFQIKLVANLEKVRVPFKMDVTTGDSIYPEVDTHYHQLMFEDKKV
                     EIPAYPTEQIIAEKLSATFSFGTDNSRAKDYYDLFTIPKLENIDTKELYKSVRNTFTK
                     RGDTKPLSFYYEREMDKIRGNDYLEKQWEKYRSVNTFANSISFTDTVNEIDHLMKSVI
                     KIENQERKRQFKRSQQMNQEMER"
     gene            complement(4722..5315)
                     /locus_tag="IWO89_002342"
     CDS             complement(4722..5315)
                     /locus_tag="IWO89_002342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="abortive infection protein"
                     /protein_id="HAQ0457060.1"
                     /translation="MQDKLKELLAIRDGNLSMKEARKFGIAATTVQRLVNQGKLIKVD
                     RGYYVEDGHGIDDLFWLQQRFSRGIFSHETTLDIYQLSTNMPKFIHLTFPHGYNVDRS
                     ITKEQQLQFHFVKKEVYELGKVEGTSFQGNPITMYDKERTLCDIWNPRYEFEYEMKLE
                     ALKEYMEDPLRDPSKLRDYMTKLHVEKEMKYHMQSLY"
     gene            complement(5556..7760)
                     /gene="topB"
                     /locus_tag="IWO89_002343"
     CDS             complement(5556..7760)
                     /gene="topB"
                     /locus_tag="IWO89_002343"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HAQ0457061.1"
                     /translation="MKTVILAEKPSQAKAYADSFSKATRKDGYFEIQDRLFSGETVIT
                     YGFGHLVELDSPDMYDENWKQWSLEHLPIFPTHYHYHVPKDKKKQFKVVKQQLQSADT
                     IIIATDSDREGELIAWTIIQQAGADHGKIFKRLWINSLEKEAIYQGFQQLRDAEETYP
                     KFEEAQARQIADWLIGMNGSPLYSLLLQQKGIPGSFSLGRVQTPTLYMIYQLQEKIRN
                     FQKEPYFEGKAQVIAQNGAFDAKLDPNETQATQEAFEDYLKEKGVQLGKQPGTIHQVE
                     TEKKSAASPRLFSLSSLQSKMNQLMKASAKDTLEAMQGLYEGKYLSYPRTDTPYITEG
                     EYAYLLDHLDEYKHFLKAEAIPTPIHTPNSRYVNNKKVQEHYAIIPTKTVMTAAAFEQ
                     LSPLQQAIYEQVLKTTVAMFAEKYTYEETTILTQVQQLQLKTVGKVPVDLGWKKLFGK
                     ESDRKEKEEEPLLPKVTKGEAVTVDLQVLEKETKPPQPYTEGTLITAMKTAGKTVDSE
                     EAQSILKEVEGIGTEATRANIIETLKQKEYIKVEKNKLVVTNKGILLCQAVEKEPLLT
                     SAEMTAKWESYLLKIGEQKGTQATFLANIQKFVSHLLEVVPGQIQSTDFGSTLQEVKA
                     ASEKQEATRHLGVCPKCREQEVLLYQNVAACTSEACDFKLWTTIAKKKLTATQLKEII
                     QNGRTSQSVRGLKGQKGSFEATIVLKEDFTTGFEFSEKKKTNYKKRTRRTTK"
     gene            complement(7771..9231)
                     /locus_tag="IWO89_002344"
     CDS             complement(7771..9231)
                     /locus_tag="IWO89_002344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toprim domain-containing protein"
                     /protein_id="HAQ0457062.1"
                     /translation="MTEKKMSLIDRCKQIDIVDFARNNGMAVVNKGRDYRLEDHDSFV
                     FDRRKQRFYWNSQNISGDIIELAKLFFIDKEIQDPKQQFKAATDFILKNEDKTERVEN
                     LHFETEKYKDHPVDYQPLTEKGRNYLKEERKLPEWLIDYAEKEGLIAELKPKHERQNF
                     LVRDDRLDHAVAFLWKDPQTNETVGASYQGTFIDYERFGERGTYKHIDKNSTANHGFN
                     LKIGDPKQLKFFESSIDLLSYAALNRDQLNDTWLVSMEGLKHHVISHYFGEAVSELRK
                     KQAFPQSIEICVDNDRAGHIFYEKEQLMGAVDPFTNQKVRCERGIANDWQVPKEYKVI
                     YEEVAKEEKITPEAIMAIHKTENNLQLTNQLVSAHKVNASFGQQLSVNDSIEAINLKD
                     ICREVAKELKACERVDGTYDFDRFYQEKGDINAQILFSYKAEQYYKGYKNHEHEFVPE
                     VKKDWNDQLKHEIYQQEIRKQKRAMLFQQGRQQERE"
     gene            complement(9292..11664)
                     /locus_tag="IWO89_002345"
     CDS             complement(9292..11664)
                     /locus_tag="IWO89_002345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0457063.1"
                     /translation="MAEEKEPLDNSRLGKSKRKLVRLQNELNEQIEKMFEHQRKTNGQ
                     PMNDKRNGHSWFRQQERLENKVHSLREEIKQQEKQVEKLERQEELKEMGYNKYGGLDM
                     TIENIPRIKEEIERFEKGESTFSAATIRKYQRKLETLEQLKERSEKGKENLLPEVQAI
                     IDSGRVTQWKKNPTIYFLKDYRKVALELNENGGFEESKKYQATSEEEKAAVRELLEEA
                     APTDSLKEKENDHFYRVEFNENDPEKGLKSYGGEIVTLELIDQLKTLDNQYAAEAGYY
                     KFYFEEVKNGEVVDQIRMDLGDGVNSNQPIYLELAKLCEQVPEKEQKQTVKNEPVSKE
                     QMQSKETKTKQKGYQKRTAEEIKQEVKSLSQQALDAVKKHTHSPEDVKELLNFMSRFP
                     ERSFRNQLLIEEQFPGATACLGRAALKKEGIYIKKGEQGNKIFVRKTVKGFYEKGRGF
                     VRETEATPEDKKRIESGQIEVIKKPYYTIEKVFDITQTQLKPEDYPKIFPNRVFDFQL
                     DKKGQSELQAGIQAVANNIGIQIRDMTESEVYQRELGQARGAYVRNEFTGEEEIVMNT
                     RNTPTQHLATSIHELAHARMHKFSELDTATKELQAEMTSYIVCKHFGMDTSEKAIPYI
                     AAWTKNGQTLDDKEAAERGKIMNDVSRVANEFIQTISNEINQYREREPEIQPAEVKQE
                     HKNTVETAEQKNIVPVGSLWIDKKDGKVMEEDTGRTLHLKDAAFIEDPQHNQVILKGK
                     SYGKEIQTALPSEEFALGNSFRFSLETVSEVAKRKNEIYYEQQLSQKERV"
BASE COUNT         2892 a         2432 c         1957 g         4386 t
ORIGIN      
        1 tgctatcact tttagttttt aggataaaaa gtgacgtcac tttttgagtg ctggtacctc
       61 aaatcctgct ttttcttggg caatcaataa atctaacgca atacacataa tcctggattc
      121 acttttgccg tacttctgct ttagttgctg caattctttt tgtctctttt catccaacgt
      181 cacgggtaac cgtttatttc ctattgcaat tggcattagt actctctcct ttcatcgaaa
      241 taaccctatt aaaaatatgg ctgcaatcaa cttgaatatt agactcacaa gggttaacac
      301 aaagcgccaa aagatcaaac aaaataccag tattcctaag aatactacga agtttaaatg
      361 tagagctgca ccatgacgca taagtatctc gccaacaaac agtacgaaca atagaggaat
      421 gattttattc atatggatct ccttctttct cttaggttat cgggctttgc ccgcttattt
      481 cgcaccacct aagcggcggg ggcggttgga tcgattggtg cgaaataaga accattccct
      541 taatttcaag gttttttgtt cttatttcac acatcctcac ttttcggtat aaatggttat
      601 tgaccttttt tcctttcaaa tcgttgatat ggcattcgtt tacggtcaag ttcattttgg
      661 atttcttctg tatcgtcgta agtgtaggtg tctaaaaaat cagttaaagt cgtgtaccag
      721 tcgtcgcctg ggttatctac ttcttctaca tattccctca aaaatgcttc gggtacagtg
      781 agttcataat ccattcccat taacacgata gattccgtcc cacacgtcaa aaatctagta
      841 aatacaaggt aataatcgtc taccgtcgca tctttgctgt ttagctcttc catttcttct
      901 tccattaatt ccatgctctc aaaaattgaa taatattctg attctgagta atagtgtttc
      961 ctgcaaatcg tgtagctgac aaagttttcg accagctcac tcctttctag gtaaacattc
     1021 atgatatatt tttctgtttc tagtcgtaga agctccgctc ctttttttaa tgtgtcagga
     1081 cgacgtttat acacgtctaa acgattatgg ctgactgtaa ccttcgtttg actcgtttca
     1141 ctttccatat acaactgttc ttccactctt tgaataaagt acttcatttc ttccatacaa
     1201 ctaaagtctt tttgaatccg tctgattact tcattcaagt gttctttgtt tgttaaaaat
     1261 tgcgaaacat cttctgttgt tagctgataa accaccttat tcattatctt tctcctccga
     1321 ttcaatcgta taaaatgcac catttttttc gatattttca aaagtatcta gccagtcgct
     1381 agccattgct ttagctttct taatctgtgc gaattttgca taagtcagtt ctaaggcttg
     1441 atattcggaa ggtttgatag tgcttggttt gacacctact tccaagcata gtaaggcttg
     1501 gaaaaaccat tttccatttt gttttttcag ttcttccagt gttcgatagt acgtcgcaat
     1561 gagattcatt tcgacacctg attcaatcat ttcttccact gcgactgaca atttttcctt
     1621 gtaatgtttt ttgggtacac gtgtttcatg gtataggaac gtcatatcct ccattttttg
     1681 tgccatttta tccaaaggta atttgacaaa ttcgtcatag taattttgag ccatattttt
     1741 tctccttttt agttctctaa tttggttaac caatattcga tttcataagt ttccatgttt
     1801 tcttgataat gcagttcata tcctgcaatc ttcactaggt cttcaaatag catagtcggt
     1861 gcatcctcta ctaaaggggt ttctatcgtc cagtaccgcg gaataagtaa agcttctgct
     1921 actttttcag aaatgacttc tttgtccacc aaaatttgaa cgacttcttt tacagtcaat
     1981 aatttgtgtt gtgttttcat caccgtttct ttactttctc atcaatcaaa acataatcgc
     2041 cataagacaa ggtatatttt ttcccgcatg ttggacattg atacaaacga tagcttttga
     2101 tacatttcgc tctttctcca caatcgcatt cgacagtatg tggtgcatga tagtttggga
     2161 ataattcgct gaagggaaca ccccaataat caaatttctt tcgcatgatt gattctgcct
     2221 cctcttagga aaggttttat ttcctttcgt tagttctttc tttattgatt tgttggccag
     2281 aatgaatcaa cgaaaagcga caaggacaag ggcgcgcagc gtgccattta cccttgtcct
     2341 tgtcgctttt tggtgataaa aggcaacttg ttgccggtct ggcaaatcaa taaagagata
     2401 gcgaacagga acgaaccttt tggtgagagg aagttttttc tattttgtct tgctttgcaa
     2461 gacaccatat cttagtgaac gtgcaacgtt cacttccttt cttctcgaac ggcggtggcg
     2521 gttggagtga gagaaaggtt tttcaattga gaaggcactt gtgtcttcct aaaaaggtct
     2581 tttcctgttt ctatgtaaaa caaaaagagc tgcaatcctc atttaggact gcagctcttc
     2641 ttttatatgc ccatcgatcg cgtaaaatga tcggggtttc taatttgcat cgattcaatg
     2701 actaattgat tccgtttatt gaaatgacct gtgacggcaa tattgtattt tccatcatcc
     2761 ataattaaca atttatcgat aatttcaata tctgcaacta cacaattaat cgattcatga
     2821 attgtttgta aagtaaagcg aaccaataat ggttgtgttt ttattactcg gattttatca
     2881 agcaaaccaa catatttgtt catcacgatc acctcgctaa aattatagca aaaacagcta
     2941 aaattgagaa tttttattcg attttagata tgctataatg atcaagacag taaagtttgt
     3001 gacggtggct atccgattga aagtgaggtg gtgcgtatgt gcgtacttcc aaaatcttac
     3061 agaaaggaga agccttttgc ctgttgaaac ggcgctagga ttaatgatta gtttcggttc
     3121 tttgatcgca acaatcattt ttggaatctt agcagttgta aacaacaatc aaaaaaaata
     3181 accgtcagct ttagccggtc gacgattatt tttcaaaaat aatattaagc tggtcaccgt
     3241 cttaaacggt gctgttaggg gagttgttag cgcaactcct cttttcgtat ccattataac
     3301 aactttattg atcttttaca atcagaatgg taaatcatca tcactaatat cgattgattg
     3361 tccgccaaat ggattttgtg aagcgttcaa tccattgttg ttaggcgtag ctacatcccg
     3421 ttgttcaaag gtggtagggc ttggactgct ggacgattga tcagcgtgcg cacggctttc
     3481 tgtcgctgct ttactttcta atagttggaa attatcggcc acaacttctg tcacatacac
     3541 acgttgtcct tgttggttat cgtaggttcg ggtttgaatc cgtccaacaa cgccgatcaa
     3601 actgcccttt ttagctaatg tcgccatcgt ttctgctggt ttgcgccaaa tcacacagtt
     3661 aataaaatca gcttctcgat tgccgttttg attcgtgaaa tttcggttga cagctagggt
     3721 aaaagtcgct actgcagtac cactcgcggt ataacgtaaa tctggatctt tggttaagcg
     3781 tcctactaaa gttacattat tgatcatctt tatctctcca tttcttgatt catttgttgt
     3841 gatcttttaa actgccgttt tctttcttga ttttcaattt tgatcacact tttcattagg
     3901 tgatcaattt cgttgactgt atctgtaaat gaaatggaat tcgcaaacgt gttaactgag
     3961 cgatattttt cccactgctt ttccaaatag tcatttcctc tgatcttatc catttctctt
     4021 tcataataaa aggacaacgg ttttgtatcc ccacgttttg taaatgtatt tcgaacactt
     4081 ttgtataact ctttcgtatc gatattttct agtttcggaa tggtaaataa gtcgtagtaa
     4141 tcttttgctc tcgaattatc tgtgccaaag ctgaacgtag cacttagctt ttctgcaata
     4201 atttgttctg ttggataagc tggaatctcc acttttttat cttcaaacat taattggtga
     4261 tagtgtgtat ctacttcagg ataaatagaa tctcctgttg tcacgtccat tttaaacggt
     4321 acacgtactt tctctaaatt agccaccaat ttaatttgaa atccaggata aaaatcggct
     4381 tctcttgttt ccttcattcc ttgaatagag aaaacaatgc cttcttttgt gggttttcta
     4441 gttaaatcat taaaaatgct cttcaatttt tcttcagtta atttgctatt tctaaatgtc
     4501 gtatcgatat caattgttgt ccttctatcg attccatatt ttgatcctaa taaaaagccg
     4561 cctttcaaaa tgaagtcatt tttgtacttt gattcagaaa tttgttcaat tagacgatcc
     4621 atcataaaat gccgttgcac taactgtgca tttaatcctg tactctttgc taaatttctt
     4681 gtttttgctg caaattgtgt cggtgtcatc tttttcctcc atcaatataa gctttgcatg
     4741 tggtatttca tttctttttc tacatgtagt tttgtcatgt aatctcttaa tttacttgga
     4801 tctctcaatg ggtcttccat gtactctttt aatgcttcta atttcatttc atattcaaat
     4861 tcatatctcg gattccaaat atcacaaagt gtacgctctt tatcatacat agtaatagga
     4921 tttccttgaa aggaagttcc ctctactttt cctagttcat aaacttcttt cttcacgaaa
     4981 tgaaattgta actgctgctc ttttgtaatt gaacgatcaa cgttatagcc atggggaaaa
     5041 gttaagtgaa tgaatttcgg catattcgta gaaagctggt aaatatctaa agtcgtttca
     5101 tgagagaaga ttcccctgga aaatcgttgc tgaagccaaa ataaatcatc tattccatga
     5161 ccatcctcta cataataacc acgatcaact ttgatcaatt ttccttgatt caccaatctt
     5221 tgtacagtgg ttgctgcgat tccaaatttc ctggcttcct tcatagataa atttccatcg
     5281 cgaatagcca acaattcttt cagtttatct tgcatttact tcccctccct tcgttacaaa
     5341 tacactacat caaatatatc atagtagttt atttgtaaca agattaacgt ctatcgatct
     5401 aattcgattt ctttttgaat gcgctgctgg ttctttaatt gttgcagtct tcttttattt
     5461 gcacgtaatt tctcttgttc taaacgaatt tttgcttttt gattttcgat ttcttgttgt
     5521 ttgtgtgttg tatttcgcat aggataaccc cttcattatt ttgtagtgcg gcgtgttctt
     5581 ttcttatagt tcgttttttt cttttcagaa aattcaaagc ctgttgtaaa gtcttctttt
     5641 aaaacgatcg ttgcttcaaa cgaacctttc tgacctttta gtcctctgac tgattggctt
     5701 gttcggccgt tttgaatgat ctctttcagt tgtgtggctg tgagtttctt tttggcaatc
     5761 gttgtccata atttaaaatc acaggcttca gacgtacatg ccgccacatt ttgataaagc
     5821 aagacttctt gttcccgaca ttttggacaa acacctaaat gacgcgttgc ttcctgtttt
     5881 tcactggctg ctttcacctc ttgtaacgtt gatccaaaat ctgttgactg gatttgtccc
     5941 ggtacgactt ccaacaagtg agagacaaat ttttggatat tagctaagaa agtagcttgt
     6001 gttcctttct gctcaccaat ctttaataag taactttccc atttggcggt catttcggca
     6061 cttgttagca gcggctcttt ttccacggct tggcaaagta aaatcccttt atttgtcacc
     6121 acaagcttat ttttttctac cttgatatat tccttttgtt tcaaagtttc aataatattt
     6181 gctcgtgttg cttctgtacc gattccttct acttctttca atatggattg tgcttcttcg
     6241 ctatccacgg tttttccagc tgttttcatc gctgtgatca acgtaccttc ggtataaggt
     6301 tgtggtggct tcgtttcttt ttcaaggact tgtagatcta ccgtcaccgc ttctcctttt
     6361 gtcactttag gaagtagcgg ttcttcttcc ttttcttttc tgtcgctctc tttgccaaac
     6421 agctttttcc aacctagatc tactggtact tttccgactg tctttagctg aagctgctga
     6481 acttgcgtca agatcgttgt ttcttcatag gtatactttt ccgcaaacat ggccaccgtt
     6541 gtttttaaca cttgttcata aatagcctgc tgcagtggac ttaattgttc aaaagccgct
     6601 gccgtcatga ccgtctttgt aggaatgatc gcgtaatgtt cctggacttt cttattattc
     6661 acatatcgac tattcggtgt atgaatagga gtaggaatgg cttcggcctt taaaaagtgt
     6721 ttgtattcgt ctaagtgatc caacagatac gcatactccc cttctgtaat ataaggggta
     6781 tctgttcgtg ggtagcttag atatttccct tcatacaacc cttgcatggc ttccaaagta
     6841 tctttggcac tagctttcat gagctgattc attttagatt gaaggctgga tagagaaaaa
     6901 agacgtggac ttgccgcact ttttttctcg gtttcaactt ggtggatcgt acctggctgt
     6961 tttcccaatt gaaccccctt ttcttttaaa taatcttcaa atgcttcttg cgtcgcttgg
     7021 gtttcattcg gatcaagctt cgcatcaaat gcgccatttt gagcgattac ttgggctttt
     7081 ccttcaaaat atggttcttt ttggaagttt ctaatctttt cctgtaattg ataaatcatg
     7141 taaagcgttg gggtttggac tctgcctaag gaaaaacttc ctggtatgcc tttttgctgc
     7201 aagagtaaac tatacagcgg gctaccgttc atgccgatca accaatcggc gatctggcgt
     7261 gcttgtgctt cttcaaattt tgggtaggtc tcttctgcat ctcgtaactg ctggaatccc
     7321 tgataaatgg cttctttttc taaggagttg atccaaagtc ttttaaatat ctttccatga
     7381 tcggcgcctg cttgttgaat gatcgtccaa gctatcagtt ccccttcacg atcgctatct
     7441 gtcgcaataa taatggtgtc ggccgattga agttgctgtt tcaccacctt gaattgcttt
     7501 ttcttatctt taggaacatg atagtgataa tgtgtaggga aaataggcaa atgctctaat
     7561 gaccattgtt tccaattttc atcgtacata tcgggcgaat ctaactcgac taaatgacca
     7621 aaaccatacg tgatcacagt ttctcctgaa aacaatcgat cttggatctc aaaataccca
     7681 tcttttcggg tagctttaga aaagctatcg gcataagcct tcgcttgtga cggtttttct
     7741 gctaaaataa cggttttcat catgacgatt tcactccctt tcttgctgtc taccttgttg
     7801 aaacaacatg gcacgttttt gctttctgat ctcttgttgg tagatttcat gcttcaattg
     7861 atcattccaa tcctttttta cttcaggtac aaattcatgt tcatgatttt tatagccttt
     7921 gtagtattgt tctgctttat aagagaaaag aatctgagca ttaatatccc ctttttcttg
     7981 ataaaaacga tcaaaatcgt acgtaccatc cacgcgttca caagctttta attcttttgc
     8041 gacttctcta caaatatctt tcaaattaat agcttctatt gaatcgttaa cagaaagctg
     8101 ctgaccaaaa gacgcattta ctttgtgagc ggaaaccaat tggttcgtta attgcagatt
     8161 gttttctgtc ttgtgtatgg ccataatagc ttctggtgtg atcttttctt cttttgcgac
     8221 ttcttcataa atgactttgt attcttttgg cacttgccaa tcattagcga ttcctcgttc
     8281 acaacgtact ttctgattcg taaatggatc aactgcgccc attaattgtt ctttttcgta
     8341 gaaaatatgt cctgcacggt cattatccac acaaatctcg atggattgag gaaaggcttg
     8401 tttctttctc aattcagata ctgcctcacc aaaataatga ctgatcacat ggtgttttaa
     8461 cccttccatc gaaactaacc acgtatcgtt taactgatca cgatttagtg cagcatagct
     8521 aagtaaatca atactactct caaaaaattt taactgctta gggtcaccga tctttaggtt
     8581 aaagccatga ttcgctgtcg aatttttatc tatatgctta tatgtgcctc tctcaccaaa
     8641 acgttcataa tcaatgaacg ttccttgata actcgcccct actgtctcat ttgtctgtgg
     8701 atctttccat aaaaaggcga ccgcatgatc taaacgatcg tctcgaacta agaaattttg
     8761 ccgttcatgt tttggcttta gttcggcaat caacccttct ttttctgcat aatcaatcag
     8821 ccactctggt agttttcgtt cttccttcag ataattcctt cctttttctg tgagtggttg
     8881 ataatcaacg gggtgatctt tgtacttttc tgtctcaaaa tgaagatttt ccactcgttc
     8941 tgttttgtct tcattcttta gaataaaatc agtggctgct ttaaattgtt gcttagggtc
     9001 ttggatctct ttgtctatga aaaaaagttt agctaattca ataatgtcgc cactaatatt
     9061 ttgactgttc caataaaaac gttgttttct tcgatcaaaa acaaatgaat catggtcttc
     9121 taagcgatag tctcgtcctt tattgactac agccattcca ttgtttcgag caaaatccac
     9181 tatatcgatc tgtttacatc gatcaataag actcattttt ttctcggtca ttttaaaacc
     9241 tcccaaaaga aaaaagacta tccgtttttc agatagtctt ttttacttgt tttatacacg
     9301 ttctttttgt gacaattgtt gttcataata gatttcattt tttcgttttg ccacttctga
     9361 cactgtctca agtgaaaatc ggaatgaatt tcctagcgca aattcttctg aaggtaacgc
     9421 cgtttggatc tcttttccat aactttttcc ttttaaaatt acttgattat gctgtggatc
     9481 ttcaataaac gccgcatcct tcaaatgtaa cgttcgaccc gtatcttctt ccattacttt
     9541 cccgtctttc ttgtcgatcc aaagtgaacc aacaggaaca atattctttt gttctgccgt
     9601 ttctacagta tttttatgct cctgcttcac ttctgctggt tggatctctg gttctctttc
     9661 tctgtattga ttgatttcat tagaaatggt ttgaatgaat tcatttgcga cacggctgac
     9721 atcattcatg attttcccac gttccgcagc ttccttatca tccaacgtct gaccattctt
     9781 cgtccatgct gcaatataag gaatagcttt ctccgaagtg tccattccaa aatgtttgca
     9841 cacaatataa gaagtcattt ctgcctgcaa ttctttagtt gcggtatcta attctgaaaa
     9901 tttgtgcatt cttgcatggg ctaattcatg aatacttgta gctaaatgct gcgtaggagt
     9961 atttcgagta ttcatgacga tttcttcttc gccagtaaat tcgttacgaa catatgcccc
    10021 ccgtgcttgt cctaattctc tttgatagac ttcactttct gtcatatctc gaatttgtat
    10081 tccaatattg ttggctaccg cttgaatccc agcttgaagt tctgactgtc cttttttatc
    10141 caattgaaaa tcaaagacac ggttcggaaa gattttcgga tagtcttcag gttttaactg
    10201 tgtttgtgtg atatcaaaca ctttttcaat cgtgtagtaa ggttttttga tcacttcgat
    10261 ttgaccactt tcgattcgtt ttttatcttc tggtgtagct tctgtttctc gaacaaagcc
    10321 acgtcctttc tcataaaatc ctttgaccgt ttttctgaca aagatcttgt tcccttgttc
    10381 cccttttttg atatagattc cttctttttt caacgcagca cgacctaaac aagccgttgc
    10441 acctggaaat tgttcctcaa tcaataattg atttctaaag gatcgttctg gaaaacgaga
    10501 cataaaattc aaaagttctt tcacgtcttc aggagaatgg gtatgctttt tcaccgcatc
    10561 cagtgcctgt tgcgaaaggg atttgacttc ttgcttaatc tcttctgccg ttcgtttttg
    10621 atagcccttt tgttttgttt ttgtttcttt cgactgcatt tgttctttcg aaacaggttc
    10681 atttttgacc gtttgttttt gttccttttc aggtacttgt tcgcacaatt ttgctaactc
    10741 taaatagatg ggttgattac tgttgacgcc atcccctaga tccattcgga tctgatcgac
    10801 tacttctccg ttcttcactt cttcaaaata gaatttataa taacctgctt ctgcggcgta
    10861 ttggttatcc aacgttttga gttgatcgat caattctaat gttacgatct ccccaccata
    10921 acttttcaat cctttttctg gatcattttc gttgaattct actcgataga aatgatcgtt
    10981 ttctttctcc ttcaaagaat cagttggcgc agcctcttca agtaattcac gaacagctgc
    11041 tttttcctct tccgacgtgg cttggtattt ttttgattcc tcaaatcccc cgttttcatt
    11101 cagttctaaa gcaacttttc gatagtcttt caaaaaataa atggttggat tttttttcca
    11161 ttgcgtcact cgtccgctgt caataattgc ttgtacttca ggaagaagat tttcttttcc
    11221 tttttctgat ctttctttca gctgttctaa tgtttccaat ttccgttggt attttcggat
    11281 cgtagcggct gaaaaagtcg attcaccttt ttcaaagcgt tcgatttctt ctttgattct
    11341 tggaatattt tcgattgtca tatccaggcc gccgtacttg ttatagccca tttcctttag
    11401 ctcttcttgt ctttcaagtt tttctacctg tttttcttgt tgcttgatct cttctctcag
    11461 actatgcact ttgttttcta aacgttcttg ttgcctaaac caagaatgcc catttctctt
    11521 atcgttcatt ggctgaccgt tggtctttct ctggtgttca aacatcttct caatttgttc
    11581 gtttaattca ttttgcaatc gtactaattt tcttttggat tttcccaagc gactattgtc
    11641 tagtggttct ttttcttctg ccatgtc
//
LOCUS       DACEEZ010000087        11093 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20313_2017
            SAMN12639966-rid9879223.denovo.087, whole genome shotgun sequence.
ACCESSION   DACEEZ010000087 DACEEZ010000000
VERSION     DACEEZ010000087.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639966
            Sequence Read Archive: SRR10023158
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11093)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11093)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741683.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741683.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:44:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,016
            CDSs (total)                      :: 2,962
            Genes (coding)                    :: 2,889
            CDSs (with protein)               :: 2,889
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 36 of 73
            Pseudo Genes (incomplete)         :: 29 of 73
            Pseudo Genes (internal stop)      :: 18 of 73
            Pseudo Genes (multiple problems)  :: 8 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11093
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639966-rid9879223.denovo.087"
                     /isolate="E20313_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(121..702)
                     /locus_tag="IWO89_002346"
     CDS             complement(121..702)
                     /locus_tag="IWO89_002346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HAQ0457064.1"
                     /translation="MKTKEKILEATKDLIYKKGYHETSIRDILAASDTGKGQFYYYFS
                     SKKEVCLAVIHAHMDVWQTELFEDILNAGKQPEEALEEMLDWIFADHETQITYHGCPV
                     GNLIIELSTVDEEYRLLLLELVNQWVKYTKQNILALSEEKLSDEEADRQAKALIAEIQ
                     GSILLLKVTQDLSLLRQNFQLIKERLLTSQKVL"
     gene            complement(885..1511)
                     /locus_tag="IWO89_002347"
     CDS             complement(885..1511)
                     /locus_tag="IWO89_002347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HAQ0457065.1"
                     /translation="MFNNKNTAVVLTDPQVEFLKPAGGGFGLTKDILEKYNTIENMIE
                     MVKQAKEKGYKLFISPHYFYPHDHKWQFGGAGEHMMLEQQMFARKSQYESVEEGSGAD
                     FIEELKPYLDENTIISSPHKIFGPESNDLALQLRKNGIDTVILGGMNGNLCSDSHMRE
                     LVESGFNVVVATDAIGAPGQEAMDAALTNFGFIADQVMTTEEVLEQLS"
     gene            complement(1533..2864)
                     /locus_tag="IWO89_002348"
     CDS             complement(1533..2864)
                     /locus_tag="IWO89_002348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-containing oxidoreductase"
                     /protein_id="HAQ0457066.1"
                     /translation="MMEKYQNIVIGFGKGGKTLAKALASKGESVLVVEKSTRMYGGTC
                     INIGCIPSKSLIFNGERGIDFTEAVARKEKLTGMLRAKNYHMISDEATGTVMDGTARF
                     LSNHQIEVTNNGEKVIVEGERIFINTGSEPIILPIKGLNTSRYLIDSTQAMDQEQLPE
                     KLVIIGAGYIGLEFASMFNEYGSKVVVLDAHSEFLPREDEDIAQMILEDMTNAGIEFH
                     LGVSVDQVADQESTAAVTFTEDGQEVTIQASKVLAATGRKPSTASLGLENTDVKLTDR
                     GAIAVDEYLRTDADNIWAIGDVKGGLQFTYISLDDYRIIMDQLKGENKRTTNNRKAVP
                     YSVFITPTLSNVGLTEKQAKAQGVNYKLFKYMTSGVPKAQVLEDPKGVFKVLVDPETD
                     LILGASIYAEDSHEVINLISLAMNGKLPYTLLRDQIYSHPTMSEALNDVLK"
     gene            complement(3019..3420)
                     /locus_tag="IWO89_002349"
     CDS             complement(3019..3420)
                     /locus_tag="IWO89_002349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="organic hydroperoxide resistance protein"
                     /protein_id="HAQ0457067.1"
                     /translation="MKKAYETAMINHGGRNGEVEAPNGSMHMKITPPGIHAEGTNPEQ
                     LFAAGYASCFNGAVQHMIKEAGMTAESTVKARVSLYNLDDGGYQIGVVLEVHIDGISE
                     EEAQKIAEDAHEFCPYSKATRGNIDVEVTIV"
     gene            complement(3506..3712)
                     /locus_tag="IWO89_002350"
     CDS             complement(3506..3712)
                     /locus_tag="IWO89_002350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0457068.1"
                     /translation="MVTNMVIFLTVLVYGVNFLVYMYLKINHIKDYVEKIAIYFGTNM
                     TLLFTSSIFLFFGKIVEDGILVFE"
     gene            complement(3807..5309)
                     /locus_tag="IWO89_002351"
     CDS             complement(3807..5309)
                     /locus_tag="IWO89_002351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomannomutase/phosphoglucomutase"
                     /protein_id="HAQ0457069.1"
                     /translation="MKDMKALQNGSDIRGIALPVEGYEVNLTTDMVKKIGWGLANWIK
                     KKKKSTDPNKKWTIGIGHDSRISGPALKEALIQSLKEQGIHVIDFELATTPAMFMSTQ
                     FEQFSCDAAVMLTASHLPYYYNGIKFFTSEGGAEKEDIAFILSQQQKIKSEQTGSVEK
                     ADLLTPYAQDLVTKIKKGIGTLESKPLNGWKIIVDAGNGAGGFFAEKVLNSLGADSTG
                     SQFLEPDGRFPNHIPNPDNKEAMESIKQAVLANEADLGIIFDTDVDRSAVVSASGTVI
                     NRNNLIAVLSTIVLKEHPGTRIVTNSPTSSHLQRFIEEKGGIQVRYISGYRNVINKAL
                     ELNRLGKDTQLAIETSGHAAFKENYFLDDGAYVIAKILMLLPELKRKGITLDEQIKEL
                     KQPVEVQEIRLKLFSDEPREQGNKILEEMKRLLEEEKGMNFDPENEEGIRYDLEEPYG
                     NGWFLIRLSLHEPLLVLQIENDEFGKNKLALVKLAKLFGHFSDLESLTAE"
     gene            complement(5327..5782)
                     /gene="argR"
                     /locus_tag="IWO89_002352"
     CDS             complement(5327..5782)
                     /gene="argR"
                     /locus_tag="IWO89_002352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine repressor"
                     /protein_id="HAQ0457070.1"
                     /translation="MKKKQRQALIRQIITEQPIGTQEELLARLHEAGADVTQATISRD
                     IREMKLVKSQNENKIVRYTLFNQPSVSLNEERLRTAIRREVLRIQSVQFMVIVLTERN
                     GADVVTNWLDEVAYPEVVGTMAGVDTFIIICRSEEEAQRFAEKLEKMRE"
     gene            complement(5880..6497)
                     /locus_tag="IWO89_002353"
     CDS             complement(5880..6497)
                     /locus_tag="IWO89_002353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0457071.1"
                     /translation="MEKIEWRKDKSYSMKKSPQILEVPKKPFFSIRGIGDPNQEDFKR
                     RVACLYAVSYAIRMSPKKNWLIPDYQMYTVYPLEGQWGLQEKYLHEPVMKKEHFSYQL
                     MIRQPDFVTEAIAQEAIQRSSKLPEDLKEQLVFKKMEEGLCAQILHVGPYAEEPATFE
                     KLEAFIAKEGYERASKEHKEIYISDPRKSAPEKMKTILRVKIEKG"
     gene            6718..8091
                     /locus_tag="IWO89_002354"
     CDS             6718..8091
                     /locus_tag="IWO89_002354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter"
                     /protein_id="HAQ0457072.1"
                     /translation="MTKQKQLLLVPAVIATGMMSFAGVLIETAMNVTFPTLIRQFDLT
                     TAQVQWVTTIYLLMISIIVPLSTYLNRNFSLKKLFLASNLLFLAGIMIDFFSPTFMVL
                     LFGRLLQGAATGIALPLMFHIILTFAPLNRRGAMMGVGTLTTAIAPAIGPTYGGIMTS
                     HFTWNHIFLFLVPVLILSLIIGWFAIPEMAVQKSGYLDWISVLGICLLFSGSLTFLST
                     LGTVSSWIALIIGGIGWFLFYYRSKKVEHPLIRLSILSNKTFRLFLIGFLVYQFLLLG
                     VSFVLPNFLQIVQGSSPFVAGLAMLPGAAVGALLSPFSGKMLDQYGPKKPIFAGLILS
                     LIGWLALFLLIGHASVGVLIACHVFYMIGIGLSYSNMMTTGMNALNEELQGDGNAMFN
                     TLQQFSGAVATSLVAVIINYVQHHTSHNYEVSTTLGSKAALGVLLLLLLVSFARFAYY
                     LFFAKKA"
     gene            8166..9278
                     /locus_tag="IWO89_002355"
     CDS             8166..9278
                     /locus_tag="IWO89_002355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HAQ0457073.1"
                     /translation="MEKIAIIGGGIIGMTLASYLDTSKFNVALFDEGTGQATKASAGI
                     ISPWLSKRRNKQWYRLAKDGAAFFPKLIKDFQLDQTIYAQSGTLLLRPEKDLAELAVL
                     AEKRKEEAPEIGNIEWLSPEQTSQALPLLKPAVSLRISGGGRLDGKAYLEHLGQRLAQ
                     KQMEQIQAKVTLHKKSDSWLIKGPFGEYTADRVILTPGPALKPLLQELGYAADIRPQK
                     GQLLAFQTSYKMSQDWPVAMLDGEADFIPFMDGKILLGATHENDQGWDLAPTSEAYKG
                     LTKSTEQFLTDPDQIKNWPYEFRVGTRAYTSDFSPFFGVLPDDPSLLAASGLGSSGLT
                     TGPYIGYLLANFLNTGHWSTEDYQKDISTYITKNPS"
     gene            complement(9350..10987)
                     /locus_tag="IWO89_002356"
     CDS             complement(9350..10987)
                     /locus_tag="IWO89_002356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxykinase (ATP)"
                     /protein_id="HAQ0457074.1"
                     /translation="MSTKESLELNEITSGNPLLSSIRSIVETTFYGNNVHEVFDRKTA
                     YQLAKGSPGTIITDLTISHAEELDLPADVRTLVFNDGSIVGRTASARRIFEDLDKEQS
                     KYEKILREAVYQSRKRQFYHTKVIVGLSEEFSVQSHLLIPEGFENNLYSYLLNFQPAN
                     SKILETYQKSVSFDEGDLYIFCDPEWTHETFPNGLILFDALHNTAAVLGLRYFGELKK
                     ATLTLAWALGNRNGFIACHGGMKQYASNDQTYTMAVFGLSGSGKSTITLAKHTEGHSV
                     SILHDDAFVISRKNGFATALEPSYFDKTQDYPSDDPAISSFLTCQNVGVTCTSKGEKV
                     LVTGDIRNGNGRTVKSRYATPDRVDHLSEKIDAVFWIMKDDSLPPIIKINDPVLAASF
                     GATLATKRSDAENITTKRQLDELVIEPFANPFRVYPLREDYQAFRELFLKQQTACYVL
                     NTGYFDDKKIKPETTLSIVERIVQQTAEFKSFGPFEDIHYLPIPDFPVSFEDTSYKNL
                     VKQRLDIRLRFIEAQIGKDLLPDEAKESLENLIQKLN"
BASE COUNT         2926 a         2356 c         1999 g         3812 t
ORIGIN      
        1 gtgttccaga agtagcttct tcgaaaaata agccgaaatt tcacaaaaat ttgagaagca
       61 attttcgtaa attccttctt atttcttgaa gctgaccact tctgtcacaa cctctaatta
      121 ttaaagaacc ttttgcgaag ttaacaatct ttctttgatc aattgaaaat tctgcctaag
      181 tagtgataga tcctgtgtga ctttcaacaa taagatactt ccctggattt cagcaatcaa
      241 cgctttggct tgacggtctg cttcttcatc ggacagtttt tcttcagaaa gtgccaggat
      301 attttgtttc gtatacttga cccattgatt gactaattct agtagcaaca gacggtattc
      361 ttcatccact gtactcaact caataatcaa attacctaca ggacagccat gataagtgat
      421 ctgtgtctca tgatcagcga aaatccagtc caacatttct tctaatgctt cttctggctg
      481 ttttccagca tttaagatgt cttcgaacaa ttctgtctgc catacgtcca tatgcgcatg
      541 gatcaccgct agacaaactt ctttttttga agaaaagtaa taataaaatt gaccttttcc
      601 tgtatcagaa gctgctagaa tatctcggat tgacgtttca tgatagcctt ttttataaat
      661 aagatctttg gtcgcttcca agatcttttc ctttgttttc aatgatattc tcctttttca
      721 tttctcatag tcctatatta tacagtaaat tcatcatttt ttcttcttaa tcgtttaaaa
      781 aagactgtgc caaattatct gacacagccc tcactataaa actgttctca tatagctaga
      841 tgttcattga ttcaagaact gcgaggacaa aagcaattcc tatcttatga tagttgctct
      901 aatacttcct ctgttgtcat cacttgatct gcaataaagc caaagttcgt caacgctgca
      961 tccattgctt cttggccagg agcaccaatt gcatctgtcg ctaccacaac attgaatcca
     1021 ctctctacca attcacgcat atgggaatcg ctacacaagt ttccgttcat tccaccaaga
     1081 attactgtgt cgattccatt ttttctcaat tggagcgcca aatcattgct ttctggtcca
     1141 aagattttat gaggagagct gatgatcgta ttttcatcca aataaggttt caattcttcg
     1201 ataaaatcgg ctcctgaacc ttcttccact gattcatatt gactttttct agcaaacatc
     1261 tgctgctcaa gcatcatatg ttctcctgca ccgccaaatt gccatttatg gtcatgtggg
     1321 taaaaataat gaggagaaat aaagagtttg tatccttttt cttttgcttg ttttaccatt
     1381 tcaatcatat tttcgatcgt attgtatttt tctaagatat cttttgtcaa accaaagcca
     1441 ccgcctgctg gtttcaagaa ctcgacttgc gggtcagtga gaactactgc tgtattttta
     1501 ttattgaaca tggtggattc tccattcgtt atttatttta atacatcatt tagagcttca
     1561 ctcattgtcg gatgagaata gatttgatct ctcaataacg tataaggaag ttttccattc
     1621 atagctagcg aaatcaagtt gatgacttca tgagaatctt ccgcatagat acttgcccct
     1681 agaatcaagt cagtttcagg gtcgactaaa actttgaata cacctttagg atcttctaat
     1741 acttgtgctt tagggacacc gcttgtcata tacttgaata atttgtaatt gacgccttgt
     1801 gctttggctt gtttttctgt taaacctaca ttggataaag tcggagtgat aaatacactg
     1861 taaggcacag ctttccggtt attcgttgtt cgtttattct cgcccttcaa ctgatccata
     1921 atgattcgat aatcatctag agagatataa gtgaactgca atccgccttt gacgtctcca
     1981 atcgcccaaa tgttgtctgc atctgtacga aggtattcat ccacagcgat tgctccgcga
     2041 tctgttagtt taacatctgt attttctaat ccaagtgatg ctgtacttgg ttttcttcca
     2101 gttgcagcca aaacttttga cgcttggatt gttacttctt ggccatcttc tgtaaaggtg
     2161 acggcagctg ttgattcttg gtctgctact tgatctacag atacacctag atggaattcg
     2221 attcccgcat ttgtcatgtc ttctaaaatc atttgcgcga tatcttcatc ttcacgtgga
     2281 agaaattcag aatgggcatc aagaacgaca actttagatc cgtattcatt gaacattgat
     2341 gcaaattcca agccaatata tccagccccg atgatcacta atttttccgg taattgttcc
     2401 tgatccatcg cttgcgtaga atcaatcaag tagcgacttg tattcaatcc tttgattggt
     2461 aaaatgattg gttcagaacc agtattgatg aaaatccgtt cgccttcaac aatcactttt
     2521 tcaccattat tcgtcacttc gatttgatgg ttagaaagaa aacgtgctgt accgtccatc
     2581 actgtcccag ttgcctcatc gctgatcata tgataatttt tggcgcgaag catgcccgtc
     2641 aatttttctt tccgagcaac agcctctgta aagtcgattc ctctttctcc attgaaaatc
     2701 aatgatttag aaggaataca gccgatgttg atacatgttc cgccatacat acgtgttgac
     2761 ttttcaacga ctaaaaccga ttcacctttt gaagctaacg ccttcgctaa agtcttaccg
     2821 ccctttccaa aaccgatcac aatattttga tatttttcca tcatttttcc ctcgcttctt
     2881 ttttacctac cgattggtac aaaaatatga taaccgataa ttgtctattc tccaaatcat
     2941 ctgctttatt tgtcaaaaaa aaccagacaa aaagcggtcc tttcacgttt ttagtctggt
     3001 tcgatttcct ttattcttct aaacaatcgt tacttctaca tcgatatttc ctctagttgc
     3061 tttggagtaa gggcagaatt catgcgcatc ttctgcaatt ttttgggctt cttcttcgct
     3121 gatgccatcg atatgcactt ctaatactac accgatctga tagccgccat catctaaatt
     3181 ataaagagag acacgtgctt ttactgtgga ctctgctgtc attcctgctt ctttgatcat
     3241 atgttgtacc gccccattga aacaagaagc ataacctgct gcaaataatt gttctggatt
     3301 tgttccttct gcatggattc ctggaggagt gattttcata tgcatcgagc catttggtgc
     3361 ctcgacttct ccattgcgcc cgccatggtt gatcattgct gtttcgtatg cttttttcat
     3421 tgtactgctc ctttcgattg accatttgct acttggctat aattatagtg tatgacaagt
     3481 aaggcgcttt ctacaaaaat tctgcttatt cgaataccag aatgccgtct tctactattt
     3541 ttccaaaaaa caaaaaaata ctactcgtga acaacagcgt catatttgtt ccaaaataaa
     3601 tagcaatctt ttctacataa tcttttatat gattgatttt aagatacata tataccagaa
     3661 agttgacccc ataaaccaat actgttaaaa aaataaccat attcgttacc attttttcct
     3721 ccttttgctg gtctccctac cagcactcga tttaaccata ccactttttt tgccgtccaa
     3781 gcaactagcc tttcaaaaag actggtttat tctgctgtta atgattcaag atcagaaaaa
     3841 tgtccgaata attttgctaa ttttactaac gccaacttgt tctttccgaa ttcatcattt
     3901 tctatctgta atactagtaa cggctcatgt aagcttaaac ggatcaaaaa ccagccgttt
     3961 ccgtatggct cttctagatc atatcgaata ccttcttcat tttccgggtc gaagttcatc
     4021 cctttttctt cttccaacag tcgcttcatt tcttctaata tcttgtttcc ttgttcgcgt
     4081 ggttcatcgc tgaacaattt taatcggatt tcttgtactt ctacgggctg tttcaactct
     4141 ttgatttgtt catctaatgt gatacctttt ctttttaatt ctggcaaaag catcaggatc
     4201 tttgcgatga catatgcacc atcatccaga aaatagtttt ccttgaatgc agcgtgtccg
     4261 cttgtttcga tggctagctg tgtatctttc ccaagacggt taagttctaa tgctttattg
     4321 attacatttc gatagccgga aatataacga acttggatgc ctcctttttc ttcaatgaat
     4381 ctttgtagat gagaagaagt aggggaattc gtcacgattc ttgttcctgg atgctctttt
     4441 aatacgattg tacttaatac tgcgatcaaa ttatttcgat tgatcactgt tccagaagca
     4501 ctgacaactg cagaacgatc cacatctgta tcgaaaatga tcccaagatc tgcttcattt
     4561 gctaaaacag cctgcttgat actttccatc gcttctttat tatcaggatt tgggatatga
     4621 ttaggaaatc ggccatctgg ttccaaaaat tgggaacctg ttgaatccgc acctaagcta
     4681 ttcaatactt tttcagcaaa gaatccacca gcaccatttc cggcatcaac aatgattttc
     4741 caaccattta gtggtttgct ttctagtgtt ccaatcccct ttttaatttt tgtgactaaa
     4801 tcttgcgcat aaggtgtcaa aaggtctgct ttttctacgc ttccagtttg ttcgcttttt
     4861 attttttgct gctgtgatag gataaaagca atatcttctt tttctgctcc tccttctgag
     4921 gtaaagaatt ttattccatt ataataatag ggaagatggc tagcagtcag cataaccgca
     4981 gcatcgcacg agaactgttc aaattgagtg gacataaaca tagcaggcgt agtagctaat
     5041 tcgaaatcga tgacatgtat cccttgttct ttcagacttt ggatcaacgc ctctttcagt
     5101 gctggtccgc ttatcctact gtcatgacca atcccaatcg tccatttttt atttggatcg
     5161 gttgatttct tctttttctt tatccagttt gctagtcccc agccgatctt ttttaccatg
     5221 tctgtcgtca agtttacttc atatccttca actggtaatg cgattcctcg aatatccgat
     5281 ccattttgca gcgctttcat atctttcata taaaagtcct cctcatttat tccctcattt
     5341 tttccagctt ttctgcaaat cgctgtgctt cttcttctga gcggcaaata ataatgaatg
     5401 tatctacacc ggccatcgtt ccgacaactt cagggtaagc cacttcatct agccaattcg
     5461 ttaccacatc ggcaccattt cgttcagtca atacgataac catgaactga acagactgga
     5521 tacgcaaaac ctcgcgtcta attgctgtac gcaaacgttc ttcatttaaa gaaacagatg
     5581 gctggttaaa taatgtatag cgtactattt tgttttcatt ttgacttttt actaacttca
     5641 tttctcgaat gtctcgagaa atcgttgctt gagtcacatc ggcgcctgct tcatgcagcc
     5701 gcgcaagcag ctcttcctgt gtcccaatag gttgttctgt aatgatctgg cggatcagtg
     5761 cttgtcgctg tttctttttc atattagaca cctctcaatg ttgattatag caaaagataa
     5821 gtaatatttc ttcatatttt ggtatttttt acttttattt tttgttttca ttcaggttgc
     5881 taaccttttt ctattttaac acgcaaaatc gtcttcattt tttcaggtgc tgattttctt
     5941 ggatcgctga tgtaaatctc tttgtgttct ttagaagctc tttcatagcc ttcttttgcg
     6001 atgaatgctt ctaatttctc aaacgttgct ggttcttcgg catagggccc gacgtgtaag
     6061 atttgggcac aaagaccttc ttccatcttt ttgaatacta gttgttcttt caagtcttcc
     6121 ggaagtttgc tagaacgctg aatggcttct tgtgctatcg cttctgtaac aaagtcaggc
     6181 tgtcggatca tcaattgata agaaaaatgt tcttttttca tcactggttc atgcaaatac
     6241 ttttcttgca gtccccattg tccttccagt ggatagacag tatacatctg ataatcagga
     6301 atcagccaat tttttttagg cgacatgcgt attgcatagc tgactgcata tagacaggct
     6361 actcttcgtt taaagtcttc ctgattcggg tcgccgatcc ctctgatgct aaagaatggt
     6421 tttttaggaa cctctagtat ttgcgggctt tttttcatac tatagctctt gtctttacgc
     6481 cattcaattt tttccatttt tcttcctccg cgaaattcca tttagttatt tattatcata
     6541 cctctttttg agtgacttgg ctaatcgtgt tgacactatt tttcagtttg tgctagaata
     6601 agtgaagtaa ataaatcgta cagataacac gtttcggcgt gtcgagagag aggcggtttc
     6661 tatttttata atagaaatca ctctcttttt ttacgaaatt ttttagaggt gatgtaaatg
     6721 acaaaacaaa aacagctgct tttagtacct gcagtaattg caacagggat gatgtctttt
     6781 gcgggcgtat tgatcgagac ggccatgaat gtaactttcc caactcttat cagacagttt
     6841 gatttaacga ctgcacaagt ccaatgggta acaacgattt atcttttgat gatttcgatc
     6901 attgttccgc tgtctactta tttgaatcgc aattttagtc tcaaaaaact ctttttagct
     6961 tcaaatctgc ttttcttggc aggtataatg atcgatttct tctctcctac cttcatggtc
     7021 ctattgtttg gtcgattgct ccaaggtgca gcgacaggta tcgctcttcc gttgatgttc
     7081 catattatct tgacatttgc ccccctcaat cgacgaggcg cgatgatggg tgtcggtact
     7141 ttgacaacgg ccattgctcc agcaattggc cccacttatg gaggcattat gacttcacat
     7201 tttacatgga atcatatctt cctgtttctt gtgcccgttt tgattctttc tttgattatc
     7261 ggctggtttg ctattcccga gatggcagta caaaaaagtg gttatttgga ttggataagc
     7321 gttttaggaa tttgtctttt gtttagtgga agtctaacat ttttgagtac gttgggaaca
     7381 gtttccagct ggattgcttt aatcatcggc ggtattggct ggttcctttt ttactatcga
     7441 agtaaaaaag tggagcatcc actgatccga ctgtctattc tttcaaataa aacattccgc
     7501 ttgtttttga ttggcttctt agtttatcag tttttattac ttggtgtatc ttttgtcttg
     7561 ccgaatttcc ttcagatcgt tcaaggaagt agtccttttg tcgctggatt agcgatgctt
     7621 cccggcgcag cggttggtgc tttgctttct cctttttccg ggaaaatgct ggatcaatat
     7681 ggtccgaaga agccaatttt tgctgggctg attctttctt tgattggttg gctagcatta
     7741 ttcctactca taggacacgc ttcagtgggt gtattgattg cttgtcatgt gttttatatg
     7801 atcggtatcg gattatctta cagcaatatg atgaccaccg gaatgaatgc actgaatgaa
     7861 gaactacaag gagacgggaa cgcaatgttc aataccttgc agcagttttc tggcgctgtg
     7921 gctacttcat tagtagcggt cattatcaat tacgtccagc atcatacttc ccataactat
     7981 gaagtgtcta ctacattagg atcaaaagca gcattaggcg tcttgttgct gttgcttctc
     8041 gtaagctttg ctcgattcgc ctattatctg ttctttgcta aaaaagcgta gattccatat
     8101 acggcaggtg ttgcatctgc cgtttttttg tgtgaaaatg gttgtttgta aaaggaggct
     8161 tttttatgga aaaaattgcc attatcggtg gcgggatcat tggtatgact ctagccagtt
     8221 acttagatac atcaaaattc aatgttgctt tatttgatga agggacagga caggcaacta
     8281 aagctagcgc tgggattatc tccccttggt tgtcgaaacg cagaaacaaa caatggtatc
     8341 gtctggctaa agacggcgct gcttttttcc ctaaacttat aaaagatttc caacttgatc
     8401 aaacgattta cgcacaatca gggacacttt tgcttcgacc tgaaaaagat ttagctgaat
     8461 tggctgttct tgccgagaaa cgaaaagaag aagcgccaga aattggaaac atcgaatggc
     8521 tttcacctga acagacatct caagcacttc cgttattgaa accagcagtt tcactgcgta
     8581 tcagtggtgg tggtcgtctg gatggaaaag catatttaga acatcttggt cagcgtttag
     8641 cacaaaaaca aatggagcag atccaggcaa aagtcacttt gcataagaag agcgattctt
     8701 ggctcatcaa gggaccgttt ggagaataca cagctgatcg tgtaattttg acacctggcc
     8761 cggcacttaa gcctttatta caagaattag gctatgctgc agatatacgt ccgcaaaaag
     8821 ggcagctctt agcatttcag acatcttata agatgagtca agactggcct gttgctatgc
     8881 ttgacggaga agctgatttc attcctttca tggatggaaa gatcctttta ggtgctaccc
     8941 atgaaaacga tcaaggctgg gatttggctc ctactagcga agcttataag ggattgacta
     9001 agagtacaga acagttcttg actgatccag atcagataaa aaactggcca tacgagttcc
     9061 gtgtaggtac ccgtgcttat acgtcagatt tttccccatt tttcggcgta ctgccagatg
     9121 atccaagctt actggctgct agcggtctag gctcttctgg cttgaccaca ggtccttata
     9181 tcggctatct attagccaac ttcttaaata ctggtcattg gtctacggaa gattatcaaa
     9241 aggacatctc cacctatatt acgaaaaatc catcatagaa aaacagcttt ttcaagtaag
     9301 gatcccgagc gatattgcgc atggattact tcttgaaaaa gctgttattt tagttcaatt
     9361 tctgaatcaa attctccaaa ctttcttttg cttcatctgg caagagatct tttcctatct
     9421 gagcttcaat gaatcgtaaa cgaatatcaa gtcgttgttt caccagattt ttataggacg
     9481 tatcctcaaa agaaacagga aaatctggta taggtaaata gtggatgtct tcaaatggac
     9541 caaacgactt gaattctgcc gtttgttgca cgattctttc aacgattgac aatgtcgttt
     9601 caggtttgat ttttttatca tcgaaatatc cagtattcag gacatagcag gctgtttgct
     9661 gtttcaaaaa caactctcga aaagcttgat agtcttccct aagaggatag acacggaaag
     9721 gattagcaaa tggttcaatg actagctcat ctagctgtct ttttgtagtg atattttccg
     9781 cgtctgatcg ttttgttgct aatgttgcac caaaagacgc tgctaaaact gggtcattga
     9841 tcttgataat tggcggaagc gaatcatctt tcatgatcca aaaaacagca tcgatctttt
     9901 cgctaagatg atcgactcgg tcgggtgtcg cataacgtga tttgactgtc cgcccatttc
     9961 cgtttcgtat atctcctgtt actaggactt tttctccttt acttgtacat gtcacgccca
    10021 cattctgaca agtaaggaaa gaagaaatgg ccggatcatc agacggatag tcttgtgttt
    10081 tatcaaagta agaaggttcc aaggcagtag caaaaccatt ttttcgagaa ataacgaaag
    10141 catcgtcatg caagatcgaa acagagtgac cttccgtatg ttttgctaag gtaatcgttg
    10201 attttccaga accggacaac ccaaatacag ccatcgtata ggtctggtca ttactagcat
    10261 attgtttcat cccaccgtgg caggcaataa agccattgcg atttcccaat gcccaagcaa
    10321 gcgtcaaagt cgcttttttt aattctccaa agtaacgtaa tcctagcaca gccgctgtat
    10381 tatggagagc atcaaacaaa atgagtccat ttggaaatgt ttcatgtgtc cattcgggat
    10441 cacaaaagat ataaagatct ccctcatcaa aggaaacaga cttttgataa gtctctaaga
    10501 tcttcgaatt cgctggctga aaattcagca aataagaata caaattgttc tcaaatcctt
    10561 ctggaatcag caaatgactt tgcacagaaa attcttcaga taggccaacg attacttttg
    10621 tatggtaaaa ctggcgcttg cgactttgat acacagcttc tcttaaaatt ttctcgtatt
    10681 tgctttgttc cttgtctaag tcctcaaaga tccgccgagc acttgctgtt cttcccacaa
    10741 tgcttccatc attgaatact agtgtcctaa catcagctgg taaatctagt tcttcagcat
    10801 gtgagattgt taaatctgtg atgatcgttc ctggactccc ttttgctagt tgataagctg
    10861 tcttcctgtc aaaaacttca tgaacattat taccgtaaaa tgtcgtttct acaatcgatc
    10921 gaatactgga taacagtgga tttccacttg tgatttcgtt taattcgaga ctctcttttg
    10981 tactcatggc tcctcctcat tccctaaaca aaaaaacgtt ttctttatta tacattattc
    11041 gagtttttat ttgtactgct gcgctgtggg aagcaaacga gttatttttc aca
//
LOCUS       DACEEZ010000088        10921 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20313_2017
            SAMN12639966-rid9879223.denovo.088, whole genome shotgun sequence.
ACCESSION   DACEEZ010000088 DACEEZ010000000
VERSION     DACEEZ010000088.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639966
            Sequence Read Archive: SRR10023158
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10921)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10921)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741683.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741683.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:44:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,016
            CDSs (total)                      :: 2,962
            Genes (coding)                    :: 2,889
            CDSs (with protein)               :: 2,889
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 36 of 73
            Pseudo Genes (incomplete)         :: 29 of 73
            Pseudo Genes (internal stop)      :: 18 of 73
            Pseudo Genes (multiple problems)  :: 8 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10921
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639966-rid9879223.denovo.088"
                     /isolate="E20313_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(<1..287)
                     /locus_tag="IWO89_002357"
     CDS             complement(<1..287)
                     /locus_tag="IWO89_002357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAQ0457075.1"
                     /translation="MKVVQTKDTMSNIYLDAVRIRHQVFVVEQGVPLSREIDKDEAHC
                     IHFVLYSDKKEPQGTVRLLPLENGKMKLQRMAILSEYRHQGLGKILIEEAE"
     gene            429..1547
                     /locus_tag="IWO89_002358"
     CDS             429..1547
                     /locus_tag="IWO89_002358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HAQ0457076.1"
                     /translation="MFEKLRQSKLMFWSLELLIIAALIMISSEINFIFEPIGTFFSTL
                     FAPVLIAGFLYYLLNPIVKLLMKAKIKRIWAVAIVLLLLVAAIIWILLSVIPSLVQQI
                     SSLASNMPDFIKQVESWLKEVAELPLFKEVDINKYFEQLDISYGTIIQQFLSGVSNSL
                     GSIVGTIASATVVIITAPFILFYMLKDGEKLVPNIQQFFPEKRRNQIVELLGQLNKTL
                     ANYISGQAIECLFVGTFTFLGYMVIGVDYAFLFGVIAGLTNLIPYLGPYLGLAPAFLV
                     TVFNDPVKALLCCVVVLIVQQLDGNIIYPNVIGKSLKIHPLTIIIVLLVAGNIAGLLG
                     IFLGVPFYAICKTIISYIIKIVKEDKQNENKKKIATTS"
     gene            1675..3051
                     /locus_tag="IWO89_002359"
     CDS             1675..3051
                     /locus_tag="IWO89_002359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010767236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyC/CorC family transporter"
                     /protein_id="HAQ0457077.1"
                     /translation="MNADPESQSLLAQILLLIVLTLINAFLAASEIAVVSINKNRIEQ
                     KADEGDVKSQKLLKILQNPNNFLSTIQVGITLVNILSGASLANTLSERLAPVLGGGTA
                     AKNIANIIVLAILTYVSIVFGELYPKRIALNKSEEVAKFTSGLIRLIGVVAKPFVWLL
                     SASTSLLARITPMTFDDEDSKMTRDEMRYMLENEGVLNNEELEMLQGVFSLDTKVARE
                     VMVPRTDAFMIDINDTVEENVNEVLSENYSRIPVYNEDKDKVVGILHTKNLLKAAHKF
                     GFDNLDIKKIMQEPLFVPETVFIDDLLYEMKKTQNQMAILLDEYGGVVGLATLEDLLE
                     EIVGEIDDESDEVENLYEKIDEHEYIIQGRMLIDEFNEAFDSDLHMSDVDTMAGYLIT
                     ALGMIPDEGEKLSFDVDNITLVSEEMEGSRILKIRVIFHDPEETEAEPEEERRYFRKE
                     FEDDEPRR"
     gene            3452..5032
                     /locus_tag="IWO89_002360"
     CDS             3452..5032
                     /locus_tag="IWO89_002360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 3"
                     /protein_id="HAQ0457078.1"
                     /translation="MNNPKLKQQVDNRRTFAIISHPDAGKTTITEQLLLFGGAIRQAG
                     TVKGKKTGNFAKSDWMEIEKQRGISVTSSVMQFDYQGKRVNILDTPGHEDFSEDTYRT
                     LMAVDSAVMVIDSAKGIEAQTKKLFQVVKKRGIPIFTFINKLDRDGREPLELLEELEE
                     LLDIESYPMNWPIGMGKGLEGLYDIYHNRVEFYRPENYQDERLVELDEDGLLPENHPL
                     TENSLYEQVLEEVELIKEAGDAFNQEKIARGDQTPVFFGSALTNFGVQTFLETFVELA
                     PAPYGHKTQDDQMVSPYEEEFSGFVFKIQANMNPAHRDRIAFVRICSGTFERGMDVWL
                     ERTNKKLKLSNVTQFMADSRENVEKAVAGDIIGVYDTGNYQIGDTLFEGKLKVAYEEL
                     PSFTPELFMKVTAKNVMKQKSFHKGIYQLVQEGAIQLYKTYLTEEYIIGAVGQLQFEV
                     FQYRMLNEYNAEVIMSPMGHKIARWIDPEDLDEKMSSSRNILARDRFDQPLFLFENQF
                     AERWFADKYPNVKLKSLM"
     gene            5230..5453
                     /locus_tag="IWO89_002361"
                     /pseudo
     CDS             5230..5453
                     /locus_tag="IWO89_002361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287661.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein"
     gene            complement(5658..5978)
                     /locus_tag="IWO89_002362"
     CDS             complement(5658..5978)
                     /locus_tag="IWO89_002362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1827 family protein"
                     /protein_id="HAQ0457079.1"
                     /translation="MKLVNVTNSHSRLVLNQLENTDAHLVKVYTAGNTTIVYTEAPEH
                     NEILLINDKRKIQPKEIEDAKNYFLDKIKDAVYHEDEIKVIELAGLVEISIPKHELGA
                     TLAV"
     gene            complement(6126..8360)
                     /locus_tag="IWO89_002363"
     CDS             complement(6126..8360)
                     /locus_tag="IWO89_002363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA domain-containing protein"
                     /protein_id="HAQ0457080.1"
                     /translation="MLCQNCGKNEATIHLYATVNGQRTQLDYCQSCYQKIKNQQNGGL
                     TNMAQNDPFGFGSLDDLFRSMSRQMQQQGNYEQTPPTQFGGNNNFNGGQPPQGNTEGL
                     LGEYGINITEQARQGDIDPVIGRDDEIKRVIEILNRRTKNNPVLIGEPGVGKTAVVEG
                     LAQKIVDGDVPQKLMDKEVIRLDVVSLVQGTGIRGQFEERMQKLIEEIRQAENVILFI
                     DEVHEIVGAGSAGDGNMDAGNILKPALARGELQMVGATTLNEYRIIEKDAALERRMQP
                     VRVDEPTVEETISILKGLQKRYEDYHHVKYTDEAIEAAATLSNRYVQDRFLPDKAIDL
                     LDETGSKKNLTIQIVDPKTIEKKLQEAEEQKVLASREEDFEKAAYYRDQINKLQKMKE
                     RQLTEEETPVITEKDMEKIVEQRTGIPVGELKEKEQTQLKNLADDLKAHVIGQDNAVD
                     RVAKAIRRNRVGLNKQNRPIGSFLFVGPTGVGKTELAKQLAYELFGSQDSMIRFDMSE
                     YMEKHSVSKLIGSPPGYVGYEEAGQLTEKVRRNPYSLVLLDEVEKAHPDVLHMFLQIL
                     DDGRLTDAQGRTVSFKDTIIIMTSNAGTGKVEANVGFGAAREGVTRSVLNQLNNYFTP
                     EFLNRFDGIIEFSALSKENLMTIVTLMLDDVNQMLAAQQLHIEVPTNVKEKLVDLGYD
                     PSMGARPLRRTIQEQIEDGIAEFYLDHPSIHELKAKLDKDGKIIVTSKPERLAKESAE
                     ETAE"
     gene            8839..9105
                     /locus_tag="IWO89_002364"
     CDS             8839..9105
                     /locus_tag="IWO89_002364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010773230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphocarrier protein HPr"
                     /protein_id="HAQ0457081.1"
                     /translation="MEKKEFHVVAETGIHARPATLLVQTASKFNSDVNLEYKGKSVNL
                     KSIMGVMSLGVGQGSDVTITAEGADEADAMAAIVETMKKEGLSE"
     gene            9105..10832
                     /gene="ptsP"
                     /locus_tag="IWO89_002365"
     CDS             9105..10832
                     /gene="ptsP"
                     /locus_tag="IWO89_002365"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HAQ0457082.1"
                     /translation="MVEMLKGIAASDGVAVAKAYLLVQPDLTFSKATVEDTAAEEARL
                     DAALAKSTEELQQIREKAAQSLGEAEAQVFDAHLMVLSDPEMVGQIKQNIKDNSVNAE
                     SALKEVTDMYIGMFEAMEDNAYMQERAADIRDVAKRILAHLLGVTLPNPSMINEEVVV
                     VAHDLTPSDTAQLDRNFVKAFVTDIGGRTSHSAIMARSLEIPAIVGTKEITAKVKEGD
                     ILAVNGIEGDVIIDPTDEQKAEFEKAGADYAAQKAEWEKLKNAETVTADGKHFELAAN
                     IGTPKDLVGVHNNGGEAVGLYRTEFLYMDSPDFPTEDDQYEAYKAVLEGMEGKPVVVR
                     TMDIGGDKELPYLQLPHEMNPFLGYRALRISLSEQGDEMFRTQMRALLRASVHGNLRI
                     MFPMVATLKEFRAAKAIFEEEKQKLISEGKEVSDTIQVGIMIEIPAAAVLADKFAKEV
                     DFFSVGTNDLIQYTMAADRMNERVSYLYQPYNPSILRLIKNVIDAAHAEGKWAGMCGE
                     MAGDQTAVPLLVGMGLDEFSMSATSILKTRSLMKRLDTAKMAELADRALKECDMMEEV
                     VELVHEYVK"
BASE COUNT         3474 a         1911 c         2214 g         3322 t
ORIGIN      
        1 ttttctgctt cttcaatcaa tatttttcct aacccttggt gtctgtattc agaaagaatc
       61 gccatccgct gtaatttcat ttttccgttt tctaaaggca aaagacgtac agttccttgc
      121 ggttcttttt tatctgagta caggacgaag tggatacaat gggcttcatc tttgtctatt
      181 tctcgagaca aaggcacacc ctgttcgact acaaaaactt gatgcctgat ccgtacagca
      241 tctaagtaaa tattgctcat tgtatctttt gtttgtacaa ctttcatggt ctagccacct
      301 tcctttcgta tagtatactg gaaaataaaa caaataaaaa gtatggcaag ttttcaaaaa
      361 taaaaaaaga ataaaagaat ttttaacgaa tataatatat actagtaaga aagaaaggag
      421 gaaatctatt gtttgaaaaa cttagacagt ctaaactaat gttttggtcg cttgaattat
      481 tgatcatagc tgcattgatc atgatttcat cagaaataaa ctttattttc gaaccgatcg
      541 ggacattttt ttccacgtta tttgcacctg tactgattgc aggctttctt tactatttat
      601 tgaatcctat tgtcaagctg ttgatgaaag caaaaatcaa aagaatatgg gctgtcgcaa
      661 ttgtgctgct ccttttagtt gcagcaatta tttggatttt acttagtgtc atcccaagtc
      721 tagtacagca aatttcctca ttggcatcta atatgcctga tttcatcaaa caggtagaaa
      781 gctggttaaa agaggtagcc gaattgcctt tgttcaagga agtagatatc aataaatatt
      841 ttgagcaatt agacatttct tacgggacaa tcattcagca attccttagt ggagtatcca
      901 atagtttagg ttcgatcgtt ggaacgatcg cttcggcaac cgtagtaatc attacagcac
      961 cattcatctt gttctatatg ttgaaagacg gagaaaaact agtgccaaac atccaacagt
     1021 ttttcccaga aaaaagaaga aaccaaattg tggagttatt ggggcaactg aataaaacat
     1081 tggctaatta cattagcgga caagcaatcg aatgtttatt cgttggtacc ttcacatttt
     1141 taggatatat ggtaattggt gtggattatg ctttcttatt tggggtcatt gcgggattga
     1201 caaacctaat tccatattta ggaccttatc taggactagc acctgcattt ctagtaacag
     1261 tcttcaatga tccggtaaaa gcattacttt gttgtgtggt tgtgctaatc gtccaacagt
     1321 tggatgggaa tatcatttat cctaatgtaa ttgggaaatc actgaaaatc catccattaa
     1381 cgatcattat cgttcttcta gtagctggaa atattgccgg attgcttggt atcttcttag
     1441 gtgttccttt ttacgcaatc tgcaagacga tcatttccta tataatcaaa atcgtcaaag
     1501 aagacaagca aaatgaaaac aagaagaaaa ttgctacgac ctcgtaggta gttttagtac
     1561 acttgttttt attgcaaaga aaattgcttt gtgataccat tttaacgtgg ctatagtgcc
     1621 acgttttttt ctatcgtaaa ctaaaaatta attttataag gagagaatgc gattatgaat
     1681 gctgaccctg agagtcagtc gctgttagcg cagattttat tattgatcgt tttgacattg
     1741 atcaatgctt ttttagcagc ttccgaaatt gctgttgttt cgatcaataa gaatcgaatc
     1801 gagcaaaaag ctgatgaagg agatgtgaaa tctcaaaagc ttttaaaaat attgcagaat
     1861 ccaaataatt tcctatcgac aatccaagta ggaatcactt tggtcaacat tttgtctggt
     1921 gcttcactag ctaatacttt atcggaacgg ttagctccgg tattaggtgg cggcacagcg
     1981 gcaaaaaata ttgcaaatat cattgtgctg gctattttga cctatgtatc gattgttttt
     2041 ggagaattgt accctaaacg tattgcttta aataaatcag aagaagtcgc aaagttcact
     2101 tcagggctga tacgcttgat tggtgtagta gctaagccct tcgtctggct gctttctgct
     2161 tccacaagtt tattggcgcg tatcacgccg atgacttttg atgatgaaga ttcaaaaatg
     2221 accagagatg aaatgcgtta tatgcttgaa aacgaaggcg tattgaataa tgaagaatta
     2281 gaaatgctcc aaggggtttt ttctcttgat acgaaagttg cccgagaagt catggtccca
     2341 cgaacggatg catttatgat tgatatcaac gacactgtgg aagaaaatgt gaatgaagtt
     2401 ttatccgaaa actattcaag aatccctgtt tataacgaag acaaagataa agtagtcgga
     2461 atccttcata caaagaattt actaaaagct gcccataaat tcggctttga taatttagat
     2521 atcaagaaaa tcatgcagga gcctttattc gttcctgaaa cggtctttat tgatgatctg
     2581 ttatatgaaa tgaaaaagac gcagaatcag atggctattt tattagatga atatggcggc
     2641 gtcgttgggt tagcaacact agaagacctt ttagaagaaa tcgtcggaga aatcgatgat
     2701 gaatcagatg aagttgagaa tctttatgaa aaaatcgatg aacacgaata tatcatccaa
     2761 ggacgtatgc tgattgatga gttcaatgaa gcttttgaca gtgatctgca tatgagtgat
     2821 gtagatacga tggctggtta tttgattaca gcattaggca tgattccgga tgaaggcgaa
     2881 aagttatcgt ttgatgtcga taatattacg ctggtttctg aagaaatgga aggttctcga
     2941 atccttaaaa tccgtgtcat ttttcatgat ccggaagaaa cagaagcgga acctgaagaa
     3001 gaacgaagat acttcagaaa agaattcgaa gatgacgagc caagaagata attggaaact
     3061 tggttatgaa agacgtgttg cttgaataaa caagcaacac gtcttttctt ttagtcagct
     3121 gacaaaaaaa tatttgactc ttatggtttg gtggcttttt cattcgctga atccatgcta
     3181 tactgattca gttgtagtag ttgacaaagg ttgtgaaaaa gctatcctgc attaagtaat
     3241 aagagcagcc aagcaaaaac agcttttcgt atttttggct tgggtgggct tattgctgat
     3301 aggctgtctc tagaacgccg tttactcgga aaaagagact gctacacggc ttttgtcaca
     3361 gccctatatg aaattgtctt tctttaagag atcggacaat ctgtgcctag agaaaatata
     3421 aataattgaa tggaagaaat ggagttaggt tatgaataat ccaaaattaa agcaacaagt
     3481 ggacaatcgc agaacttttg cgatcatttc ccatccggat gctgggaaaa caacgattac
     3541 agaacaatta ctattgtttg gtggggctat ccgtcaagcc gggaccgtaa aaggcaaaaa
     3601 aacaggtaat tttgccaaat cagactggat ggaaatcgag aaacaacgtg ggatttctgt
     3661 tactagttct gttatgcagt ttgattatca agggaaacga gtaaatatct tggatacacc
     3721 aggacatgaa gatttttctg aagatactta tcgtactttg atggccgtgg atagtgcggt
     3781 catggtaatc gatagtgcaa aagggatcga agcacagaca aagaaattat tccaagttgt
     3841 aaaaaaacgt ggcatcccga tatttacttt tatcaacaaa ctagatcgtg atggtcgtga
     3901 accattggaa ttgttagaag aattagaaga gctattagat atcgaatctt atccgatgaa
     3961 ttggccaatc ggtatgggta aagggctaga aggtctttat gatatctacc ataatcgtgt
     4021 agagttttat cgtccggaaa attatcagga tgaacgatta gtagaattgg atgaagatgg
     4081 actgcttccc gaaaatcatc ctctaactga aaattcctta tatgaacaag tcttagaaga
     4141 agtagaacta atcaaagagg ctggagatgc atttaatcaa gaaaaaattg ctcgtggcga
     4201 tcagacacca gtattttttg gttctgcatt gactaacttc ggtgtacaga cttttttaga
     4261 gactttcgtt gaattggcac cagctcctta tggacacaaa acacaagatg atcagatggt
     4321 aagtccttat gaggaagaat tttcaggttt cgtattcaag atccaagcaa atatgaatcc
     4381 agcccatcgt gaccgaatcg cttttgtccg aatctgttct ggaacattcg aacgaggaat
     4441 ggatgtctgg ttggaacgta caaataaaaa actgaaatta agcaatgtca cacaatttat
     4501 ggcagattcg agagaaaacg tcgaaaaagc agtagcaggc gatatcatcg gtgtatatga
     4561 tacaggcaat tatcagattg gggatacatt gttcgaagga aaactaaaag ttgcttatga
     4621 agaactccct tcatttactc cagagctgtt tatgaaagtg actgcaaaaa atgtcatgaa
     4681 acaaaaatcc ttccacaaag gaatctatca attagttcaa gaaggagcga ttcagcttta
     4741 taaaacatat ctaacggaag aatatatcat tggagctgtt ggtcagctac aattcgaagt
     4801 attccaatat cgtatgttaa atgagtataa cgccgaagta atcatgtctc caatgggaca
     4861 caaaatcgct cgctggatcg atccagaaga tttggatgag aagatgagtt caagtcgcaa
     4921 tatcttggct cgtgaccgtt tcgaccaacc gttgttctta ttcgagaacc aatttgcaga
     4981 acgctggttt gcggataaat atcccaatgt aaaattaaaa agtttgatgt aaagaagtct
     5041 tgacacaagc tgccctgcat caagtaataa gaatagccaa acaaaaacag cctctcatat
     5101 tttgtttaga gccgtgacgg attttccgtg acggctcttt ttactatatt aaaatcatag
     5161 aaatgtctaa tcaaaaaaat ataaaaatta ttttgcgtac atatgattaa agaaaggaga
     5221 gtgtgacaca tggaaacaga atggcaaaga cgtgaaagaa aaaaacagaa aaagaagaga
     5281 tgttacgatt atgtcgatta ttagatgtaa aagactggga caaaaacgag caaattgcac
     5341 aaaaagtacg atcaatcatc cataaagagg aaaaagttaa aaataaagac acgcgtagaa
     5401 ctgaaaatga aaagagaaaa gtacaaaaga aaagagaaaa atcaaaaagc tagaaagaaa
     5461 aagaggctga gacagaagcg tttaactcca agaaataagc tggaattcac aaaaatttga
     5521 aaagcaattt tcgtgaattt cagcttattt ccgaaggagt tgcttctgct ttcgccgttt
     5581 atacgtgttt agagcgtgag acaaaagtgt tctttacttt tgtcccacgc tctttaatta
     5641 ttatgttcaa aatttgttta cacggctaat gtagcgccta attcatgttt tggaatcgag
     5701 atttcaacaa ggcctgccaa ttcgattact ttgatttcat cctcgtggta aacggcgtct
     5761 ttgattttat ctaagaaata atttttagca tcttcgattt ctttcggctg gattttacgt
     5821 ttgtcattga tcagcagaat ttcattatgt tcaggggctt cagtgtagac aatagtggta
     5881 tttccggctg tgtagacctt gactagatgg gcatcagtgt tttcaagctg gtttaataca
     5941 agacgggaat ggctgtttgt tacatttacc aatttcatgg cgctcacctc ttttatgtat
     6001 ttttaattgc ttgtttctct tattataatt tcttttttat aaaatgaaca gtattttgac
     6061 ttttctgaaa aaagaatgag acggcatttc tcaaagaaat accgtctcac gctataaaac
     6121 tggatttatt cagccgtttc ttcggctgat tctttagcca aacgttctgg tttagaagta
     6181 acgatgattt ttccatcttt atccagtttt gctttcaact catggataga agggtgatca
     6241 agataaaatt cagcgatacc atcttcgatt tgttcttgga tcgtgcgtcg aagaggacga
     6301 gcacccatag atggatcata gcctaagtca actaattttt ctttgacatt cgtaggaact
     6361 tcgatgtgaa gttgctgtgc tgccagcatt tggttgacat cgtctagcat tagtgtgacg
     6421 attgtcatca agttttcttt tgataatgca ctgaattcga tgatcccgtc aaaacggttc
     6481 agaaattctg gtgtaaagta gttgtttaat tggttcaata cagaacgagt aacaccttcg
     6541 cgtgccgcac caaaaccaac gtttgcttct actttaccag ttccggcatt acttgtcata
     6601 atgatgatcg tatctttaaa gctgaccgtt cttccttgtg catctgtcaa tcgaccatca
     6661 tctaagattt gcaagaacat atgcaaaaca tctggatgag ctttttctac ttcatctaac
     6721 aagacaagac tgtaaggatt tctccgtact ttttctgtta attgaccagc ttcttcgtat
     6781 ccgacataac ctggaggaga accaatcaat ttagagacac tatgtttttc catatattca
     6841 gacatatcga aacgaatcat tgaatcttgt gaaccgaata gttcgtaggc aagttgtttc
     6901 gcaagctctg ttttacctac accagttggt ccaacaaata agaaggagcc gattggacga
     6961 ttctgtttgt ttaatccaac acggtttcgg cgaatcgctt tcgctactcg atctacagcg
     7021 ttatcttgtc cgatgacatg agctttcaag tcatctgcta agtttttcag ttgtgtctgt
     7081 tctttttctt ttaattcacc cactggaata cctgtccgtt gttcaacgat tttttccata
     7141 tctttttcgg taatcacagg tgtttcctct tcagtgagct gacgttcttt cattttttga
     7201 agtttattga tctgatcgcg ataataagct gctttttcga aatcttcttc tcgagaagct
     7261 aaaacttttt gttcttctgc ttcttgcagt tttttctcga ttgttttagg atcaacgatt
     7321 tggatcgtca agtttttctt agaacctgtt tcatctagca aatcgatggc tttatccggc
     7381 aagaagcgat cttggacgta acgattggat aaagttgcgg ctgcttcgat ggcctcatca
     7441 gtatatttta catgatgata gtcttcataa cgtttttgca aacctttcaa aatactgatc
     7501 gtttcttcga cagttggttc atctacccgt acaggttgca tacgacgttc taaagcagcg
     7561 tctttttcga tgatgcggta ctcatttaag gtagtagcac caaccatttg caattctcct
     7621 cgagctaaag ctggttttaa aatattacca gcatccatat ttccatcacc agcagaacca
     7681 gcaccgacga tttcgtgaac ttcatctatg aataggatca cattttctgc ttgacggatc
     7741 tcttcaatca atttttgcat acgttcttca aattgtcctc gtataccagt accttggact
     7801 aaagatacga catctaaacg aatgacttct ttgtccatta atttttgcgg tacatcacca
     7861 tctacgattt tttgtgctaa accttcaaca actgctgttt taccaacacc tggttctcca
     7921 atcaagacag gattgttctt ggttcggcga tttaaaattt caatgactcg tttgatttcg
     7981 tcatcgcgtc cgatcacagg atcgatatca ccttgtcgtg cttgttctgt gatattgatt
     8041 ccgtattctc ccaataaacc ttctgtattg ccttgaggtg gctgaccacc attgaagttg
     8101 ttgtttccgc caaattgtgt tggtggtgtt tgctcataat ttccttgctg ttgcatttgg
     8161 cgagacatgg aacggaaaag atcgtccaaa ctgccaaatc caaatggatc attttgggcc
     8221 atattagtca gacctccatt ttgttgattt ttgatttttt gataacagct ctgacagtaa
     8281 tcgagttgtg tccgctgacc attgacagtg gcatacaagt gaatcgttgc ctcattcttg
     8341 ccgcagtttt gacaaagcat gaatcattca cgtcctttca caaaaatggg tacatccata
     8401 ttttacatct cgtttttcat gaggtaaaag aataacccct agttttttaa gagatttatg
     8461 aggtagaaaa ggtgaaatct ctattgacca ttcctgacct ttgactctta ttatactaaa
     8521 aataaaaagg gaagcaagta attcgtttca tatgaaaaca aagagggtca aaggaaagaa
     8581 tgataaacgt caggagaaga gaaagcgaca agaaaaagga aataaacacg tttgttataa
     8641 aatgtgaaga aaaggatgaa aaagaatccg gaataatttg aatactttta tctgaaattg
     8701 aatgaaaact gttgtatcta acgaaaaaaa atgttaatct ttacagatga aagggtggca
     8761 tttgttagga ttctctgaat tactaaaaaa gaaaaccctc acataaaaac aacactcact
     8821 taaaggagac cgattaatat ggaaaagaaa gaatttcacg tagtagcaga aactgggatc
     8881 cacgctcgtc cagctacact attagtacaa acagcaagca aatttaactc tgatgtaaac
     8941 ttagagtata aaggtaaatc agtaaacttg aaatcaatca tgggcgttat gtctttaggt
     9001 gtaggtcaag gttctgatgt tactattact gcagaaggtg ctgacgaagc tgatgcaatg
     9061 gcagcaatcg ttgaaacaat gaagaaagaa ggcttatctg aataatggtt gaaatgctaa
     9121 aagggattgc cgctagtgac ggtgtagccg ttgcaaaagc ttacctgcta gttcaaccgg
     9181 atttaacatt cagtaaggca acagtagaag atactgcggc tgaagaagct cgtttggatg
     9241 ctgcattagc taaatctact gaagaattgc agcaaattcg tgaaaaagca gcgcaaagct
     9301 taggtgaagc agaagcgcaa gtatttgatg cccatttgat ggttctttca gatccagaaa
     9361 tggtaggtca aatcaagcaa aatattaaag acaacagcgt aaatgctgaa tcagcgctaa
     9421 aagaagtaac ggatatgtat atcggtatgt tcgaagcaat ggaagataat gcttatatgc
     9481 aagagcgtgc tgcagatatt cgtgacgttg ctaaacgtat tttagcgcac ttattaggcg
     9541 ttactttacc aaatccatca atgatcaacg aagaagtggt cgttgttgca catgacttga
     9601 ctccaagtga tacagcacaa ttagatcgca attttgtaaa agcgtttgta acagacattg
     9661 gtggacgtac ttcacattct gctatcatgg cacgttcttt agaaattcca gcaatcgttg
     9721 ggacaaaaga aattactgct aaagtcaaag aaggcgatat tttggctgtc aacgggatcg
     9781 aaggcgatgt aatcatcgat cctacagatg aacaaaaagc tgaatttgaa aaagcaggtg
     9841 cagattacgc agcacaaaaa gcagaatggg aaaaattaaa aaatgcagaa actgtcacag
     9901 ctgacggcaa gcattttgaa ttagctgcaa acatcggaac accaaaagat ctagttggtg
     9961 tccataataa tggtggagaa gcagttggtc tgtaccgtac tgaattcctg tatatggatt
    10021 ctccagactt cccaacagaa gatgaccaat atgaagctta caaagctgta ttagaaggta
    10081 tggaaggaaa acctgtagtt gttcgtacaa tggacatcgg tggagataaa gaactaccat
    10141 atctgcaatt acctcatgaa atgaatccat tcttaggata tcgtgcattg cgtatcagtt
    10201 tatctgaaca aggcgacgaa atgttccgta cacaaatgcg tgcattgctt cgtgcatctg
    10261 ttcatggtaa tttacgtatc atgttcccta tggtagctac actcaaagaa ttccgcgcag
    10321 caaaagcgat ctttgaagaa gaaaaacaaa aattgataag tgaaggcaag gaagtatctg
    10381 atacgatcca agtcggcatc atgatcgaga ttccagcagc agcagtctta gcagacaagt
    10441 ttgcaaaaga agtagatttc ttctctgtag gtacgaacga tttgattcaa tacacaatgg
    10501 cagcagaccg tatgaacgaa cgcgtttctt acttgtacca accatataac ccatcaattt
    10561 tacgtttgat caaaaatgta attgatgcag cacacgctga aggaaaatgg gcaggtatgt
    10621 gtggagaaat ggctggagat caaacagctg ttccattgct agtaggtatg ggtctagatg
    10681 agttctcaat gagtgcgaca tctattctta aaacgcgtag tttaatgaaa cgcttggaca
    10741 cagcgaaaat ggctgaactt gctgatcgtg cattgaaaga atgcgatatg atggaagaag
    10801 tcgttgaact tgttcatgaa tatgtaaaat aaacaacgta tcaaaagctg gtgtggcttg
    10861 taaaagagct acgccagttt ttttgttctt ttttaagaaa acgtttttgt cagtaagaat
    10921 a
//
LOCUS       DACEEZ010000089        10432 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20313_2017
            SAMN12639966-rid9879223.denovo.089, whole genome shotgun sequence.
ACCESSION   DACEEZ010000089 DACEEZ010000000
VERSION     DACEEZ010000089.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639966
            Sequence Read Archive: SRR10023158
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10432)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10432)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741683.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741683.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:44:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,016
            CDSs (total)                      :: 2,962
            Genes (coding)                    :: 2,889
            CDSs (with protein)               :: 2,889
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 36 of 73
            Pseudo Genes (incomplete)         :: 29 of 73
            Pseudo Genes (internal stop)      :: 18 of 73
            Pseudo Genes (multiple problems)  :: 8 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10432
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639966-rid9879223.denovo.089"
                     /isolate="E20313_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(142..894)
                     /gene="xth"
                     /locus_tag="IWO89_002366"
     CDS             complement(142..894)
                     /gene="xth"
                     /locus_tag="IWO89_002366"
                     /EC_number="3.1.11.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HAQ0457083.1"
                     /translation="MKFISWNVNGLRAIVNKNFLEVFHELDADFFCLQETKLQAGQID
                     LDLPGYYQYWNYAERKGYSGTAIFAKKPALNATYGMGIDIHDTEGRLITLEYSDFFLV
                     TCYTPNSQSELKRLDYRLEWEEAFYNYLENLKKQKPVIVCGDLNVAHQKIDLKNWKTN
                     QKNAGFTPEERAALSRLLDNGFIDTFRYFYPTQEGVYSWWNYRFNSRKNNAGWRIDYF
                     LTSKDLEPGLADAKIHTDIMGSDHCPVELDLK"
     gene            1007..1927
                     /locus_tag="IWO89_002367"
     CDS             1007..1927
                     /locus_tag="IWO89_002367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HAQ0457084.1"
                     /translation="MKIGVIGVGNIAEKAYLPTYAKKQGTLDFYFATRNKETKERIQK
                     EYGFQHLYETIDELLEEKIEACMIHAATKVHYELAKKCLENGIHVYIDKPLSVHLNEV
                     RELQELADKNQIILMIGFNRRFAPMVEELKRIPDKRLIHLQKNRIAAKETTEFVLYDL
                     FLHLIDTAVYLLDEPILRTKYQIRETKEFLEYAILQLETQNQTAILTMDLASGANIEK
                     YQVTSKNGTYEVNDLTQLVIQEADGKKVKEFGDWTNTLVKRGFEPMVEAFFSQIRLGK
                     SETELLKQKEVVRSHELCEEVLKDHYRHQL"
     gene            2000..2935
                     /gene="murB"
                     /locus_tag="IWO89_002368"
     CDS             2000..2935
                     /gene="murB"
                     /locus_tag="IWO89_002368"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HAQ0457085.1"
                     /translation="MNKEEIVESLATITILFDEPLMNYTFTKTGGPADVLAFPKKQEE
                     VKQIIDYCRIHDIPWMVLGNASNLIVQDGGIRGVVIMLTEMKQIHVKGTMVIAEAGAS
                     LIDTTYAALAESLTGFEFACGIPGSVGGAVYMNAGAYGGEIKDVFAEVDLLLEDGTLK
                     TLTKDEMVFSYRHSKVQELRAIVLEARFSLQTGDYEAIKARMDELTELRQSKQPLEYP
                     SCGSVFKRPVGHYTGQLIQQAGLQGLKWGGAQVSEKHAGFIVNIDHATATDYIELIAH
                     IQEVIKEKFDVSLETEVRIIGEKQAISETSRSLAN"
     gene            3233..4711
                     /locus_tag="IWO89_002369"
     CDS             3233..4711
                     /locus_tag="IWO89_002369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M protein trans-acting positive regulator"
                     /protein_id="HAQ0457086.1"
                     /translation="MKLDYLIEEEDREKLLLARYLEFEKKNYVVLPKLYETLGLSKFK
                     TRNYIIELNKEFTEFKTKPEIQISEEAEIHVERLNLTDIKNLRRIYFQRSKIARLLTE
                     IVDSKVTIEKFAENHFLSISRAYVKRRELVSFLEQHQIKLKKNILVGEEINIRNVLFG
                     VYFGVFNGFSMPFRESVMNEAKSIVQYFEYLFNLKLSATKRIKFNLLTTISLLRAYGN
                     QPIGKRFFSDEYFKSSEGMKIIKDLSHISFLHIPKEQIIDEISYIMLFLITEEEFELD
                     GAFGLAEAHFHDIHMISQKMSVELIDQLPFSIEISEEERLYIRKELRMGMMHVNRKNW
                     IFDFEMTSFTSKKQLQFFFETYPIFSTAIQKVIQENKRYFLGKDERFMIRLFYDYLFL
                     LVGICPVSYFEAPIHVCIDFSQGSAYTDYIISQVKGFKNYNIVIESRITSRTNIYLSD
                     CLLEKFNKEQIIWKNPPTPDDWEYFGNTIIREKNRHLTEKIS"
     gene            4988..7048
                     /locus_tag="IWO89_002370"
     CDS             4988..7048
                     /locus_tag="IWO89_002370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0457087.1"
                     /translation="MCSKRRSKQLNKHACRLDKERSCSEKHYVKFMMRTMLISLSVGV
                     YMHVPVQATTQTIKVVRTSVSETTDSPITITHTFTDANGKQLLPALTSYNKVSYPPTI
                     NHYWLIPNKTLWTSETGSSVPLSIFSDMTDPTKGDQLKQITDILNHALTDIKDGGSAQ
                     VSYVYEKDYSQFERKKIDVLAGTKVSISDLVERLKNADGTQGDMSKVISNNGNIVDTT
                     NTGTSTISLSYFDPIALKTMTASAVIHVRADQTAIKGKDLHLRTGESYAPKDLVEYVT
                     KSDGTSGNKEEIKMNGTVDTTTPGEYPVVLTYIDSKTSNVAETTAVVYVEASAASSTV
                     ITHFIDSQTNKELQAETHMSSVNRIPDLRLPAEFSDRVPNIDLSTYEQNGKVTSISDW
                     MQANGLDRKSHWSEILEVLHSELMNNRKQSGGEITFTYVYIPDNRSLEGHDLIVHPNE
                     KINKEELIQSATNSLGKPVPLKDVSVTINEVPLEDGYLTKQTGDFTIMYSYHDPYSLQ
                     ELHAQSLLHVLPLNKQIDQKEQKRREESLSEKKQQMAHAVSPTGKVKQTRSLELARIS
                     RKISESNKTIRQEAHLAPQVSGLENEILEPKIKQQSEKEFVAKQSEDQKESDDQKETK
                     KKRLSTIDSTDEHLQPQVPLPSPGTPAPGGGIPSSALGDFMRGMAGTWVYANRNEFDA
                     DDWF"
     gene            7131..7700
                     /locus_tag="IWO89_002371"
     CDS             7131..7700
                     /locus_tag="IWO89_002371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAQ0457088.1"
                     /translation="MLDKTLPYAEIWMTRPLQQNVPITPLAEGFEFRFYQKEDESAWA
                     EIETSVGEFQTTDEALEYFQQNFAPYPEELERRMFFVETKKGEKVATCTAWRKKSREQ
                     VYPVFHWLAVKPDYQGKGLAKALTARVLQEFPTLHTDGPIYLHTQTWSHQAIALYKKM
                     GFTFIAENLDGTPNPDYEKVMQVLSKLDK"
     gene            complement(7875..8681)
                     /locus_tag="IWO89_002372"
     CDS             complement(7875..8681)
                     /locus_tag="IWO89_002372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0457089.1"
                     /translation="MNHLKTFFTNSTKTAGRITNGIYILLRIIVVIMLVDQVWNQHWG
                     NTMLLLLTLILFEIPYFLEKILKIQIPNMLEIILLSFIFSSTVLGELSDFYGYFSMWD
                     TFLHAFSGFLAGGVGFSLVYLLNKNTKTMNLTPLLLAVVSFCFSMTVGVMWEFVEFSA
                     DKLLQFDMQKDTIIQQINSVELSKDGNTVYHVPDITRTIIESQNADGKTDKTIIQNGY
                     LDVGIMDTMKDLFVNFIGAVVFSVFGYLYSRNDYQKFTFIKNFIPKKIKR"
     gene            8819..10333
                     /locus_tag="IWO89_002373"
     CDS             8819..10333
                     /locus_tag="IWO89_002373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavocytochrome c"
                     /protein_id="HAQ0457090.1"
                     /translation="MKKLVAGLLTAVALLAMSACGADQKNTAQTSSSTKESAEVSSGA
                     SEQVYTDPKEMKESYDVVIIGAGGAGMAAAIEAKDAGLNPVILEKMPVAGGNTSKSSS
                     GMNASETKFQKEEGIKDSNDAFYEETLKGGGGTNDKEMLRYFVDHSADAVDWLDQNGI
                     KLDNLTITGGMSEKRTHRPSDGSAIGGYLVEGLLNNVHEREIPIFVNADVTDIKKNGE
                     TVNEVTVHVQGEEPKEVTGKAIVVTTGGFGASKEFIEKYRPDLADYITTNQEGSTGDG
                     ITMIEKLGGQTVDMDKIQIHPTVQQDEGVLIGEVVRGEGAILVDQEGKRFVNEMDTRD
                     KVSAAITALPEKSAYLIFDQGVRDRATAIEFYDQKGYVTTGKTMEELAEKIDLPKETL
                     AKTVDGWNTDVAEKKDAQFNRATAMEHDLSSPNYYAIKIAPGIHYTMGGVKINTNTEV
                     LDKENQPIRGLYAAGEVTGGLHGNNRIGGNSVAEIIIFGRQAGIQAARFASENE"
BASE COUNT         3701 a         1739 c         2057 g         2935 t
ORIGIN      
        1 acagcttttt cacaatctct cgataaacag tgttcaaaag ccagtttctc ggtattatgc
       61 caaatttatg caaaatgtgg aaagccattt tcacaaaatt tttcttaata ctcgaaacta
      121 aacgactttt tcacaacctc tttatttcaa atctaattct actggacaat gatcactgcc
      181 cataatgtcc gtatggatct ttgcatcagc tagtcctggc tctaaatctt tgctcgtcaa
      241 aaagtaatcg atccgccaac cagcattgtt tttccttgaa ttgaatcggt agttccacca
      301 agagtagaca ccttcctggg ttggataaaa ataacggaat gtatcgataa acccgttatc
      361 caacagtcga gaaagagccg ctctttcttc tggagtgaat ccagcatttt tttgattcgt
      421 tttccagttt ttcaaatcaa tcttttgatg agcgacattt agatccccac acacgatgac
      481 tggtttttgt ttttttagat tttccaaata attataaaag gcttcttccc attctaatcg
      541 gtaatctaaa cgttttaatt cgctttgaga atttggcgtg taacaggtta caagaaagaa
      601 atcggaatat tccaaagtaa tcagacggcc ttctgtatca tggatatcga tacccattcc
      661 gtaagtggca ttcaatgctg gttttttagc aaaaatcgct gtgccggaat agccttttcg
      721 ttccgcgtaa ttccaatatt gataataacc tggaagatct aaatcaattt gtcctgcttg
      781 gagtttcgtt tcctgcagac agaaaaaatc ggcgtctaac tcatgaaaaa cttctaaaaa
      841 atttttattt acgatggcac gaagcccgtt tacgttccaa gagataaatt tcaataaaag
      901 cacactcctt cgaattaatt tccttacctt tgcgatacta ttgtacctca ttttagtaaa
      961 aaatggtgta agatgaaagt aactaaatga tccaggagga aagtagatga agattggtgt
     1021 aatcggtgta gggaatatcg cagaaaaagc atatttacca acttatgcga aaaaacaagg
     1081 aacacttgat ttttattttg caacaagaaa taaagaaaca aaagaaagaa ttcaaaaaga
     1141 gtacgggttc caacatctat atgaaacgat cgatgagctt ttagaagaaa aaatcgaagc
     1201 atgtatgatc cacgcagcaa caaaagtaca ttatgaatta gccaaaaaat gtctagaaaa
     1261 tggtatccat gtttatattg acaaaccact gagtgtccat ctaaatgaag ttcgtgaatt
     1321 gcaagaatta gctgataaga accaaataat tttgatgatc ggtttcaacc ggcgctttgc
     1381 tcctatggta gaagaattga aacgtatccc agataagcgg ttgatccacc tgcaaaaaaa
     1441 tcgaatagcg gcaaaagaaa cgacagagtt cgtcctttat gatttgtttt tacatcttat
     1501 tgatacagct gtgtatttat tagatgaacc cattttgaga acaaaatacc agataagaga
     1561 aacaaaggaa tttttggaat atgccatttt acaattagaa acacaaaatc aaacggctat
     1621 tttaacgatg gatttagcaa gtggcgctaa tatagaaaaa tatcaagtaa caagtaaaaa
     1681 tggtacttat gaagtaaacg atctgaccca gcttgtcatt caagaggctg atggaaaaaa
     1741 agtgaaagaa tttggcgact ggacaaacac gctagtcaaa cgcgggtttg agccaatggt
     1801 agaggccttc ttttctcaga tccgtttagg taaatcggaa acagaattgc ttaaacaaaa
     1861 agaagttgta agaagtcatg aattatgtga ggaagtgctt aaagatcact accgtcatca
     1921 actttgaaca agactggcaa gattgtatta agcgtgatag aataatgagg acgtttgaga
     1981 aaagaaagga aaatcctcag tgaataaaga agaaatagtt gaaagcttag ccacgattac
     2041 tatattgttt gatgaaccat tgatgaacta tacgtttacg aaaacaggtg gaccagctga
     2101 tgtccttgca ttcccgaaaa aacaagaaga agtaaagcaa atcatcgatt attgccgaat
     2161 ccatgatata ccttggatgg tattaggaaa tgcaagcaat ctgatcgttc aagatggcgg
     2221 gattcgcggt gtagtcatca tgctgactga aatgaaacag attcatgtca aaggaacgat
     2281 ggtcattgcg gaagcaggtg cctcactgat tgatacgact tatgcagctt tggcagaatc
     2341 attgactgga tttgaatttg cttgcggcat cccgggaagt gtcggtggag cggtttatat
     2401 gaatgcaggt gcgtatggcg gagagatcaa agatgttttt gctgaagtgg atcttttgct
     2461 tgaagatgga acattgaaaa ctttgacaaa agacgaaatg gtattttctt atcgtcacag
     2521 taaagtccaa gaattacgtg ctatcgtatt ggaagctcga ttcagtttgc aaacagggga
     2581 ttatgaagca atcaaagcaa gaatggatga gttgaccgaa ttgcgtcaat caaaacagcc
     2641 attagaatac ccttcatgcg gaagtgtctt taagcgccca gtaggacact atacaggtca
     2701 gctgatccaa caagcaggat tgcaaggcct gaaatggggc ggtgcacaag tctctgaaaa
     2761 acatgctgga ttcatcgtca atatcgatca tgcgacagcg acagattata ttgaattgat
     2821 tgcgcacatc caagaagtaa tcaaagaaaa atttgatgtg tctttagaaa cagaagtgcg
     2881 gatcattgga gaaaaacaag caatatcaga aacaagcaga agtttagcca attaataagt
     2941 aatggaactt aaaataagta aaagagcagg acggtggccg tcctgctctt ttttgtggtt
     3001 gtttacgcaa atggcgcaaa tcttgtacat tttaacacaa taaaacaaaa aatatagaat
     3061 atttcctcat aaatttttcc attttttgca atattctcaa aaaaaagata aaaaatgaga
     3121 ataactgatg aaaaaacaag cataatccat cataaaatca gtataggtga aaagatgaac
     3181 tttcatctgc atatcaagta attggtatga ggcatttttt gaggtgtagg atatgaagtt
     3241 ggattacctt atagaagagg aagatagaga aaaactgctg ctagctagat atttagaatt
     3301 tgagaaaaag aattatgtag tgctgcctaa gttatatgaa acattaggtt tgtctaaatt
     3361 taaaacaaga aattatatca tagaattaaa taaagaattt actgaattca aaacaaagcc
     3421 agagatccaa atttcagaag aagcagagat ccatgtcgaa cgtctgaatt tgacggatat
     3481 caaaaattta agaagaatct actttcaacg atcaaaaatt gctcgactgt taacggagat
     3541 cgtggattca aaagtaacga tcgaaaaatt tgcagagaat cattttttaa gcatatcaag
     3601 agcctatgta aaaagaagag aattagtctc ttttcttgaa cagcatcaaa taaagttaaa
     3661 gaaaaacata ttagttgggg aagaaataaa catacggaac gtattatttg gggtatattt
     3721 cggggtcttt aatggttttt ctatgccatt tagagaaagc gtcatgaatg aagcaaagag
     3781 tatcgtgcaa tattttgaat atctgttcaa tttaaaactg tctgcaacaa aacgaatcaa
     3841 attcaatttg ctaaccacta ttagtttgtt gagggcctat gggaatcaac caatcggaaa
     3901 acgtttcttt tcagatgaat actttaagag ctcagaagga atgaagatca tcaaggattt
     3961 atcacatatt tcttttcttc atatccctaa agagcagatc attgatgaga tttcttatat
     4021 catgttgttt ttgatcacgg aagaagaatt tgaattagat ggcgcatttg gacttgcgga
     4081 agctcatttt catgatatcc acatgattag tcaaaaaatg tcagtggaat tgattgatca
     4141 attaccattt tcgattgaaa tctctgaaga agaaagacta tatatacgaa aagaacttcg
     4201 tatgggtatg atgcatgtca atcgaaaaaa ttggatattc gactttgaga tgactagctt
     4261 tacgtctaaa aagcagctac agtttttctt cgagacgtat ccaatattca gtacagcaat
     4321 ccaaaaggtc atacaagaga ataaacgcta tttcttggga aaagatgaac gtttcatgat
     4381 tcgcttattt tatgattatc tctttttatt ggtcggtatt tgtccggtat catattttga
     4441 agcaccgatt catgtctgta ttgatttttc gcaaggatct gcttataccg attacattat
     4501 ttctcaagtg aaaggattta aaaattacaa tattgtgatt gagagtcgaa tcacttctcg
     4561 tacgaatatt tatttatctg attgtttact tgaaaaattt aataaagaac agataatttg
     4621 gaaaaatcca ccgactccag atgactggga atattttgga aataccatca tcagagaaaa
     4681 aaatagacat ttaactgaaa aaatcagcta gtatttatgg atggcagctt tgtctcacat
     4741 atttattagc tgatttatgt ttttttcaaa gaaaaggtaa ctagatataa ctagatataa
     4801 ctagatatat tacttaacaa aattaaaaag tacataagtt attattccga ttttcttgaa
     4861 acaataaaaa atcaaaaaaa atactctcct aatagtccat cttcttttga ttttatttat
     4921 ataatgaaat catcttatga aaattactac ttcatgttta gacaagagta gggaggacta
     4981 agaataaatg tgttcaaaaa gacgttcaaa acaactaaat aaacacgctt gtcgtttaga
     5041 taaggaacgg tcgtgttctg aaaaacacta cgttaagttt atgatgcgca cgatgctgat
     5101 cagtttatct gtaggtgtct atatgcatgt acccgttcag gcaacgacac aaaccatcaa
     5161 ggtcgtacgt acctctgttt ctgagaccac tgattcccct atcactatta ctcatacttt
     5221 cactgatgca aatggaaaac agctacttcc tgcattgact tcctataata aagtcagtta
     5281 tcctccaacg ataaaccact actggttaat tcctaacaaa acattatgga catcggaaac
     5341 cggaagcagt gtgccgcttt ctatcttttc cgatatgact gatcctacaa aaggtgatca
     5401 gttaaaacag atcactgata ttttgaatca tgctttgaca gatataaagg acggtggaag
     5461 cgcacaagtt tcttatgtat atgaaaaaga ttattcacag tttgaaagaa agaaaatcga
     5521 tgttctggca gggacaaaag tttcgatatc tgatctggta gaaaggctga agaacgcaga
     5581 tggaacacaa ggagatatgt ccaaagtaat ttcaaacaat gggaatatcg tggatacgac
     5641 taatacagga acatctacta tttccttgtc ctattttgat ccaattgctt taaaaacaat
     5701 gactgcatct gctgtgatcc atgttcgtgc tgatcaaaca gctatcaaag gaaaagatct
     5761 tcacttaaga acaggagaga gttacgcccc taaagatctt gttgaatatg taacaaaaag
     5821 cgatggaact tcaggaaata aagaagaaat caaaatgaac gggacagtag atactacaac
     5881 tccgggagaa tatcccgttg tattaactta tatagactcg aaaacatcaa atgtggcaga
     5941 aacaacagct gtagtttatg tagaagcatc tgctgcttca tcgactgtca tcactcattt
     6001 tatagatagt caaacaaata aagaacttca agcagaaaca catatgagta gtgtaaatcg
     6061 tattcctgat ttgcgcctac ctgctgaatt ttctgatcga gtgccaaata tagatttatc
     6121 gacttatgaa caaaatggga aagtcacttc tatttctgat tggatgcaag caaacggatt
     6181 agacagaaaa agtcactggt cggagatcct tgaagtactt cactcagaac tgatgaataa
     6241 taggaagcaa tcaggagggg aaattacctt tacttatgtt tatataccag acaatcgttc
     6301 attagaaggt catgatctta ttgttcatcc aaacgaaaaa ataaacaaag aagagttgat
     6361 ccaatcagca acaaattcct taggtaaacc cgtaccgtta aaggatgtat cggttacaat
     6421 caatgaagta ccattggagg atggttattt gaccaaacaa acaggtgatt ttacgattat
     6481 gtatagttat catgacccgt attcactcca agaactccat gctcagtcac tattacatgt
     6541 tttaccatta aacaaacaga tagatcaaaa agaacaaaaa agacgagaag aaagcttgtc
     6601 agaaaaaaaa caacaaatgg ctcatgcagt ttctccaaca gggaaagtaa aacaaacaag
     6661 aagcctagag ttagcaagaa tatctagaaa aatctcagaa tctaataaaa cgataaggca
     6721 agaagctcat ttggccccac aagtttctgg attggaaaat gaaattttag agcctaaaat
     6781 aaaacaacaa tcagaaaaag aatttgttgc taaacagtcg gaagatcaga aggaatcaga
     6841 tgaccagaag gaaacgaaga aaaaacgctt gtcaactatt gattccaccg atgagcatct
     6901 gcagccgcaa gtaccacttc ctagtccggg aacacccgca cctggtggag gtattccttc
     6961 ttctgcttta ggagatttca tgcgaggtat ggcaggaaca tgggtatacg ctaatcgaaa
     7021 tgaatttgac gcagatgact ggttctgatt tatatctcta tctgttatag tcaaataagt
     7081 agctgagtca tatgttattt ggcataatgg agacgaggag ggatagaaaa atgttagata
     7141 aaacgttacc ttacgcagaa atctggatga caagaccact tcaacaaaat gttccaatca
     7201 cgcctttagc ggaaggtttt gaatttcgat tttatcaaaa agaagatgaa tctgcttggg
     7261 cggagattga gacttccgtt ggggaatttc aaacgacaga tgaagcactt gagtattttc
     7321 aacaaaactt tgctccttat cctgaagaac ttgaaagaag gatgtttttt gtagagacaa
     7381 aaaaaggaga aaaagtagca acctgtaccg cttggcgaaa aaaatcaaga gagcaagtgt
     7441 atccggtttt tcattggtta gcagtgaaac cagattacca aggaaaaggg ctcgcaaaag
     7501 cattgactgc cagagtcttg caagaatttc ctaccctcca tacggacgga ccaatctatc
     7561 ttcacacaca gacatggagc catcaggcga ttgctcttta taaaaaaatg ggttttactt
     7621 ttattgctga aaatctagac ggcacaccta atccagatta tgaaaaagtt atgcaggttt
     7681 taagtaaatt agataaataa cgaaatggtg aaataagtcc tcagtattgg gataaaaaac
     7741 gaaatagtca ttcctcatat cttacttttt tgagttaata ctatcggata actaatggca
     7801 tgccatacat tcaaaaaaga aggggcagtg acaaaagtat tgtcactgcc ccttctcttg
     7861 ttttttagca tgttttatcg tttgattttt ttaggaataa aattcttgat gaacgtaaat
     7921 ttttggtaat cgtttcgact atagaggtaa ccaaatacac tgaaaacaac tgctccaata
     7981 aaattaacaa ataaatcctt catagtatcc atgattccca cgtctaagta accattttgg
     8041 atgattgttt tatctgtttt accatctgca ttttgacttt caatgattgt ccgagtgata
     8101 tctggaacat gatagacagt gtttccgtct ttacttaact ccacactatt gatttgctgg
     8161 atgatcgtat ctttttgcat atcgaattgc agtaatttat ctgctgaaaa ttccacaaac
     8221 tcccacataa caccaactgt catcgaaaaa cagaaggaaa caactgctag taataaagga
     8281 gtcaagttca tggtttttgt atttttattg agcaaataaa caagggaaaa gccgacaccc
     8341 ccagctaaaa atccgctgaa tgcatgtaga aaagtatccc acattgaaaa atagccataa
     8401 aaatcactta actctcctaa aactgttgaa ctaaaaataa aagataacaa aatgatttct
     8461 agcatatttg gaatctgtat ctttaatatc ttttccagaa aataagggat ttcaaataaa
     8521 attaatgtca gtaataaaag cattgtattt ccccagtgtt ggttccagac ctgatccact
     8581 agcataataa cgacgatgat acgtaataaa atatagattc cgtttgtgat tctacctgca
     8641 gtttttgtac tgttcgtaaa aaaggttttc aaatgattca taaaataact ccttcatgta
     8701 tatccttaat tatactaaga agcgtgtgtt taccaaagct atatgactta aagaaaaaca
     8761 gagttgatat tgtgaaaaaa atcacttaat atagtataag gagaaaaggg ggatacaaat
     8821 gaaaaaatta gttgctggtt tattaacagc cgttgcactt cttgctatga gcgcatgcgg
     8881 cgcagatcag aaaaatacag cgcagacaag ttctagtacg aaggaatctg ccgaagtatc
     8941 atcaggcgct tctgaacaag tatatacaga tccaaaagaa atgaaagaat cttatgatgt
     9001 cgtgattatt ggagctggtg gtgcagggat ggcagcagct atcgaagcaa aagatgctgg
     9061 gttgaatcca gtaattttgg aaaagatgcc tgttgccgga ggaaacacat ctaaatcatc
     9121 aagcggtatg aatgcttcag aaacaaaatt tcaaaaagaa gaaggaatca aagactcgaa
     9181 tgatgccttc tatgaagaaa cgctaaaagg tggtggagga acgaatgata aagaaatgct
     9241 tcgatacttt gtcgatcact ctgctgatgc ggtcgattgg ctagatcaaa acggtatcaa
     9301 actggacaac ttaacgatca ctggagggat gagtgaaaag aggacacatc gtccgtctga
     9361 tggctcggct attggtggat acttagtgga ggggttattg aataacgtcc atgagcggga
     9421 aatccctatt tttgtcaacg cagatgtgac agacattaag aaaaatgggg aaacggtcaa
     9481 tgaagtgact gtacatgttc aaggagaaga accaaaagag gtcaccggaa aagcaatcgt
     9541 agttaccaca ggcggttttg gtgccagcaa agaatttatt gaaaaatatc gtcccgattt
     9601 agctgattat attacgacaa atcaagaagg atcaactgga gatggtatca ccatgattga
     9661 aaaattaggt ggacaaaccg ttgatatgga taaaatacaa attcatccaa ctgtgcagca
     9721 ggatgagggg gtcttgattg gtgaggttgt tcgcggagaa ggagcaatct tggtcgatca
     9781 agaggggaaa agatttgtca atgaaatgga tacaagggat aaagtatctg cagcaattac
     9841 agcattgcct gaaaaatcag cttatttgat ttttgaccaa ggcgtgcgtg atcgtgccac
     9901 agctattgaa ttttatgacc aaaaaggata tgtcacaact ggaaagacaa tggaagaatt
     9961 agcagaaaaa atcgatttac caaaagaaac actagcaaaa acagttgatg gatggaatac
    10021 ggatgttgcg gagaaaaaag atgctcaatt caaccgtgcc acagccatgg aacatgactt
    10081 atcgtctccg aattattatg cgatcaaaat cgcaccagga atccattaca cgatgggcgg
    10141 tgtgaaaatc aatacgaaca ctgaagtatt agataaagaa aaccagccaa ttagggggtt
    10201 atatgctgct ggtgaagtca caggtggctt acatggaaac aaccggatcg gtggaaattc
    10261 agttgcggag atcattattt ttgggcgaca agcgggcata caagcagctc gatttgcttc
    10321 ggaaaatgaa tgaaatagaa cgtaaataaa aagaggttgt gaaatcagcg ttttgagcta
    10381 ataccgaggg tcagctgatt gaacactgtt tagtgaagag gttgtgaaaa ag
//
LOCUS       DACEEZ010000090        10086 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20313_2017
            SAMN12639966-rid9879223.denovo.090, whole genome shotgun sequence.
ACCESSION   DACEEZ010000090 DACEEZ010000000
VERSION     DACEEZ010000090.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639966
            Sequence Read Archive: SRR10023158
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10086)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10086)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741683.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741683.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:44:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,016
            CDSs (total)                      :: 2,962
            Genes (coding)                    :: 2,889
            CDSs (with protein)               :: 2,889
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 36 of 73
            Pseudo Genes (incomplete)         :: 29 of 73
            Pseudo Genes (internal stop)      :: 18 of 73
            Pseudo Genes (multiple problems)  :: 8 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10086
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639966-rid9879223.denovo.090"
                     /isolate="E20313_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(247..759)
                     /locus_tag="IWO89_002374"
     CDS             complement(247..759)
                     /locus_tag="IWO89_002374"
                     /EC_number="2.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine phosphoribosyltransferase"
                     /protein_id="HAQ0457091.1"
                     /translation="MDLKDYIASIPDYPSKGIVFRDISPLMADGDAYREATKQIVNYA
                     KEKRIDMVVGPEARGFIVGCPVAYELGVGFAPVRKKGKLPRETIEVTYGLEYGTDTLT
                     LHKDAIKPGQRVLICDDLLATGGTIKATIELIEQLGGVVVGCAFLIELMDLHGRDKIK
                     GYDIITLMEY"
     gene            complement(773..3070)
                     /gene="recJ"
                     /locus_tag="IWO89_002375"
     CDS             complement(773..3070)
                     /gene="recJ"
                     /locus_tag="IWO89_002375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded-DNA-specific exonuclease RecJ"
                     /protein_id="HAQ0457092.1"
                     /translation="MRQANFDWKLSETEPSESFLQMIRELKLSPFVGKLLWQRGYHEE
                     KDINRFLHPKEQELHDPYLIHDMDKAISRIQEAVISGEKILVYGDYDADGITSATVMK
                     ETLELLGAEVEVFLPNRFEHGYGPNQSVYQEKIEEGIQLIITVDNGVAGNDAVAYAQQ
                     AGVDVIITDHHELPENLPDAYAIVHPRHPAGHYPFPDLAGVGVAFKVASALLEEPPAE
                     FLDLVAIGTIADLVSLTDENRILVSLGIDAIHHSERIGLQALFEESGVKMRDADETTI
                     GFSIAPRLNAIGRMGDPRPAVSLLATFDEEEASVQAKKLNEINEERKAIVEQITQEAL
                     AMVNKENHIHLLVNRGWHEGVLGIVAGKIMNETGKPTLVLTLKEDGSAKGSGRSIEAL
                     NLFEMLDQMRDLFTYFGGHHAAVGLTMPSENVTILQEKMNQYIVDHQIDLMRGPELRI
                     DEVLLPNEVTVERIDELKLLAPFGTDNPLPQFLFRQVQAENIKRIGANQQHLKFVLSD
                     ASSQLDAVAFGFGTQEEELLNNQVDVVGKLSINEWNGRKKPQLMVSDFSVGGLQVFDW
                     RAKRFREQSRTSEGSLYLAFDSSTLKFVPEIIQEAAIVFEGLDSVKAIIEKRKPNSLV
                     VMDCPDDLADLKEVFRSYSFNRVYLMGISPDEAYLNGVGTREQYAKLFKLIHSQERID
                     IRHKIKAIAQYLRIPEKLLIFMIQVFFELKFVTIENGVLQKVASPESHPLTESRLYQR
                     RLNKIKVEEFLLLSDIPTIKKWLTT"
     gene            complement(3212..3646)
                     /locus_tag="IWO89_002376"
     CDS             complement(3212..3646)
                     /locus_tag="IWO89_002376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1049 domain-containing protein"
                     /protein_id="HAQ0457093.1"
                     /translation="MKKQSNVILGFVLVLIIVLFAVLNNKNVPVSFGFTSFSAPLILV
                     IIGSALIGALIVFLTASATLWQQKKQIKQLTQEMSEYQTELQKKIEEAKEEQQREFRN
                     ERAELEAAYQAELQAKTAQISQLKAPEEQKTQSPKQSFNYFD"
     gene            complement(3656..4444)
                     /locus_tag="IWO89_002377"
     CDS             complement(3656..4444)
                     /locus_tag="IWO89_002377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HAQ0457094.1"
                     /translation="MNLEDKVVLVTGSSGGLGAQICYEAAKQGAIVISCARRMAFVEG
                     VRDECRRLSGKEAYAFKVDVSNPESVDELYEKVMEEVGRVDILVNNAGFGIFEDFLTF
                     DLGKAYDMFEVNILGMMVLTQKFAIDMAERRQGHIINIASMAGKMATAKSTVYSATKF
                     AVLGFSNALRLELKPLGVAVTTVNPGPIETNFFDKADPSGSYLEKVGQIVLEPAKLAK
                     MIVRNMRHPKREINQPFILEVASKFYTLFPTIGDYLASGIFNKK"
     gene            complement(4441..5382)
                     /locus_tag="IWO89_002378"
     CDS             complement(4441..5382)
                     /locus_tag="IWO89_002378"
                     /EC_number="3.1.26.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Z"
                     /protein_id="HAQ0457095.1"
                     /translation="MELQFLGTGAGVPAKHRNVTSIALKLLDERNEVWLFDCGEGTQM
                     QILRTTIRPRKIGKIFITHLHGDHIFGLPGLISSRSFQGGDTPLEIYGPKGIEEYIKV
                     SLGISQTRLSYPLKFIELNETAPIFTDQQFSVYTKKLNHGIDSFGYRVVEHDHKGELQ
                     VDRLKELGIPAGPLYGKLKQGETIQLEDGRTINGKDFVGPDKKGRIVTILGDTRKTHN
                     SVVLAENSDILVHESTFNKDEARMAHNYFHSTTHQAAEVAKEAQAKRLLLTHISARYL
                     GKAALELEKEAQEVFENTTIMKDFDSIEIPFREEEEA"
     gene            complement(5559..6146)
                     /locus_tag="IWO89_002379"
     CDS             complement(5559..6146)
                     /locus_tag="IWO89_002379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1269 domain-containing protein"
                     /protein_id="HAQ0457096.1"
                     /translation="MSKRVIILNFQVESQAYQAFSEIKKLHAVRSIKGEQMAVVSHDP
                     NGTHQFKIEDFIDFTGNNKSSTGGLIGMLVGVLGGPLGILLGWFAGGMIGATQDAKEI
                     KDAQTVFDFVGNKIGEGATGLILIADEEDNRPLNQLVMMELGGEITRLDYEDVEKDVH
                     DAKEAENHAKEAAQKTWKDKHPQTSNDNESSGSSN"
     gene            complement(6367..7680)
                     /gene="obgE"
                     /locus_tag="IWO89_002380"
     CDS             complement(6367..7680)
                     /gene="obgE"
                     /locus_tag="IWO89_002380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003126661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase ObgE"
                     /protein_id="HAQ0457097.1"
                     /translation="MSMFLDQVTIDVKAGKGGDGMVAFRREKYVPDGGPAGGDGGRGG
                     DVILIVDEGLRTLMDFRFNRHFKAQPGENGMSKGMHGRGSEDTYVKVPQGTTVRDAET
                     GALLGDLIENGQTLVVAKGGRGGRGNIRFASPRNPAPEIAENGEPGQERKIELELKVL
                     ADVGLVGFPSVGKSTLLSVISSARPKIGAYHFTTLVPNLGMVTTSDGRSFAAADLPGL
                     IEGASQGVGLGTQFLRHIERTRVILHVIDMSGMEGRDPYEDYLAINKELSTYNLRLLE
                     RPQIIVANKMDMPDAPENLVKFKEQLNKEKEDEFADDIPVFPISGVTRQGLDALLNAT
                     ADLLEVTPEFPLYEEELEEETVHYGFNPEGPEFQIDRDSDATWILSGEKIEKLFQMTN
                     FDHDETVMRFARQLRGMGVDEALRARGAKDGDLVRIGEFEFEFVE"
     gene            7968..8978
                     /gene="gap"
                     /locus_tag="IWO89_002381"
     CDS             7968..8978
                     /gene="gap"
                     /locus_tag="IWO89_002381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HAQ0457098.1"
                     /translation="MTVKVGINGFGRIGRLAFRRIKEVSDDIEVVAINDLTSPTMLAQ
                     LLQFDSTHGTYPGTVTATEDSIVVDGEATRVYAEPDASKIPWAKENGVDIVLECTGFY
                     TSEEKAKAHLDAGVKRVVISAPAGAMKTIVYNVNDDTLDENDKIISAGSCTTNCLAPM
                     AYFLNNEFGIEVGTMTTVHAYTSTQMLLDGPVKGGNLRAARSAADNTIPHSTGAAKAI
                     GLVIPELQGKLQGHAQRVPVVDGSLTELVSVLKTKVTADQVNEAIKKHTVDNPSFGYD
                     DRQIVSSDVIGTTQGSIFDPTQTEVTTAGDFQLVKTVAWYDNEYGFTCQMIRLLEKFA
                     NL"
     gene            complement(9063..9998)
                     /locus_tag="IWO89_002382"
     CDS             complement(9063..9998)
                     /locus_tag="IWO89_002382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HAQ0457099.1"
                     /translation="MKLWMKILLGVALALIVALVFAGNYFYNYAVVPSEKDFLDEDTP
                     GTTQTSKETDAHKWFSSKENRSDWTITSKDGLKLSAIYLPAETKSEKTALVAHGYMGN
                     AETMTNYAKMFHDMGYNVLVPDARGHGKSEGDYIGFGWPERKDYVQWINKVLENNGKS
                     QEIVLYGVSMGAATVMMTSGEKLPDNVKAIIEDCGYSSVHDELAYQLDDMFSLPAFPL
                     MQVTSLVTKVRAGYFFGEANAVEQLKKNQRPMLFIHGDEDTFVPFEMLDKVYRATKGP
                     KEKYVVKGAEHAEAYKTDPEKYQQVVQQFLSQYVH"
BASE COUNT         2777 a         2298 c         1746 g         3265 t
ORIGIN      
        1 ttttacgggg gtgtgacaaa aatcggtcac atcctttttt tcgaataaac ggtgttctaa
       61 agccagtttt tcggtattaa gtcaaattgc ggaaaaagtg attttttaaa cttctactgg
      121 tctttgcttg tttccattta taaaaaaata gcgaccctca aaagtcagat agagaatctg
      181 acttttgagg gtcgctttaa aatcgcttct ttaagaataa catcaagaat agcttctttt
      241 ttctgcttaa tattccatta atgtaatgat gtcatatcct ttgattttgt cgcgtccatg
      301 gagatccatc aattcgatca aaaatgcgca gcctacgact acaccgccta attgttcgat
      361 cagttcgatc gtcgctttga ttgttcctcc agtagctagc aaatcgtcac aaatcagcac
      421 acgttgacct ggtttaatgg catccttatg caatgtcaat gtatctgttc catattctaa
      481 tccataagtc acttcgatgg tttctcgagg taacttccct tttttgcgga ctggggcaaa
      541 accgactcct aactcataag caacaggaca tcctacgatg aatccacggg cttctggccc
      601 aactaccata tcaattcgtt tttcttttgc ataatttacg atttgtttag tagcttcccg
      661 ataagcgtca ccatctgcca tcagtgggga aatatctcga aacacaattc cttttgacgg
      721 atagtcggga atactggcaa tataatcttt taaatccact ctttgtttcc tcctaggttg
      781 ttaaccattt ctttatggta ggaatatcgc tcaaaagcaa aaattcctca actttgattt
      841 tattgagccg tcgctggtat aatctgctct cagtcaaagg atgactttct gggctagcga
      901 ctttttgtaa aacaccgttc tctattgtaa caaatttcaa ttcaaaaaac acctgtatca
      961 taaaaattaa taatttttca gggatacgca aatattgagc tattgctttt attttatgac
     1021 gtatatcaat tcgttcttga gaatggatca atttaaacaa ttttgcgtac tgttctcttg
     1081 tgcctacccc attcaaatag gcctcatcag gtgaaatccc catcagataa acacgattga
     1141 atgaataaga ccgaaaaact tctttcagat ccgctaaatc atctggacaa tccataacaa
     1201 ctagagagtt tggttttctt ttttcaatta ttgcctttac agaatctagt ccttcgaaaa
     1261 caatcgcagc ttcctggata atctcaggca cgaatttcaa cgtcgaagaa tcaaatgcta
     1321 aataaaggct accttccgac gttcggctct gttcacggaa acgttttgcc cgccaatcaa
     1381 atacttgcag accacccaca gaaaaatcag ataccatcag ctgcggtttc ttacgcccgt
     1441 tccattcatt gatcgaaagc tttcctacca catctacctg attattcagc agttcttctt
     1501 cttgagtacc aaaaccaaaa gcgaccgcat caagctgact cgatgcatcg gaaaggacaa
     1561 atttcagatg ctgttggttt gctccgattc ttttgatatt ttctgcttgg acttgtcgaa
     1621 ataaaaactg aggcaaagga ttatccgtac caaatggcgc caatagtttc agctcatcaa
     1681 ttcgctctac tgttacttca tttggcagca atacttcatc aattctcaac tcaggccctc
     1741 tcatcaagtc aatttgatga tcgactatat attggttcat cttctcctgt agtatagtga
     1801 cattttcact tggcatcgtt aaaccgactg ctgcatgatg tcctccgaag taagtaaata
     1861 aatctcgcat ctggtcaagc atttcaaaaa gattcaaggc ttcaatacta cgtccagatc
     1921 ctttagctga tccatcttct tttaaagtaa ggacaagcgt tggtttaccc gtttcattca
     1981 ttattttccc agcaacaatg cccaaaacgc cttcatgcca tcctcggttt accaatagat
     2041 ggatatgatt ctccttattg accatcgcta atgcttcttg tgttatctgc tctacgattg
     2101 cttttctttc ttcattgatc tcattcaatt tttttgcttg aacggatgct tcctcttcat
     2161 caaacgtcgc aagtaacgag acggcaggac gtggatcgcc catccgaccg attgcattta
     2221 gtctgggagc aatcgaaaaa ccgatcgttg tttcgtctgc gtccctcatt tttacgccgc
     2281 tttcttcaaa tagcgcctga agtcctatcc gttctgaatg atgaatggca tcgattccta
     2341 atgaaacaag gattcgattc tcatcagtca atgaaactaa atcggcgatc gttcctatcg
     2401 ctactaaatc caaaaattct gcaggcggtt cttccaataa tgcagaagct actttgaatg
     2461 ctactccgac tcctgccaaa tctggaaatg gatagtgccc cgctggatga cgagggtgaa
     2521 caatcgcata agcgtctggt aaattctcag gtagttcgtg atgatctgta atgatcacgt
     2581 ctacaccagc ttgctgagcg tatgctaccg catcatttcc tgctaccccg ttatcaacag
     2641 tgatgatcaa ctggattcct tcttcgatct tttcttggta taccgattgg tttggtccat
     2701 acccatgctc aaatcgattt ggcaggaata cttctacttc tgcaccaagt aattccagtg
     2761 tttctttcat taccgttgca ctggtgatcc catctgcgtc ataatcaccg taaacgagga
     2821 ttttttctcc actgattaca gcttcttgga ttcggctgat tgctttatcc atatcgtgta
     2881 tcagatacgg atcatgtaat tcttgttcct ttggatgtaa aaatcggttg atatcctttt
     2941 cctcatggta acctctttgc cacagtaact taccgacaaa tggagataat ttcagttcac
     3001 ggatcatttg gagaaaggat tcagaaggct ctgtctcgct taacttccaa tcaaaatttg
     3061 cttgtctcac tttctcactc ctaacttagt tattatagca aaggaaatca gcaaaaaaaa
     3121 gcagaaagaa agaataaaaa cagatcagat ccgctagaca gatgttcagc aggacctgat
     3181 cttttgtacg accatttatg aaatatccct attagtcaaa ataattgaag ctttgtttgg
     3241 gcgattgagt tttttgttct tccggcgctt tcaattgact gatttgtgcg gttttcgctt
     3301 gtaactccgc ctgataagca gcttccaact ccgcacgttc gtttctaaac tcgcgttgct
     3361 gttcttcttt tgcttcttca attttctttt gaagttctgt ttgatattct gacatttcct
     3421 gagtcagctg tttgatttgc tttttttgct gccatagtgt tgccgaagca gtcaaaaata
     3481 caatcagtgc accaatcagc gcagaaccga tgatcactaa gatcaatggc gcagaaaagg
     3541 aagtgaagcc aaaacttaca ggaacatttt tattgttcaa tacagcaaaa agaacaataa
     3601 tcaaaactaa aacaaatcca agaatcacat tgctttgctt tttcaattcc ttcacctact
     3661 ttttgttaaa aattccgctg gctaaataat caccaatcgt tggaaacagc gtataaaatt
     3721 ttgatgcaac ttctaaaata aacggttgat tgatttcccg ttttggatgt ctcatattgc
     3781 ggacgatcat ttttgccaac ttggcaggtt ctaaaacgat ttgtccgact ttttccaaat
     3841 agcttccgct tggatcagcc ttatcgaaga aatttgtctc gattggacct ggattcactg
     3901 tcgtaacagc tacgccaagt ggtttcaatt ctaatcgcaa agcgttggag aagccaagga
     3961 ctgcaaattt cgtggcagaa taaaccgttg attttgcagt tgccattttt ccagccatag
     4021 aggcgatatt aatgatatga ccttgtcttc tttctgccat atcgatcgca aatttttgtg
     4081 tcaaaaccat cattcctaag atgttgactt caaacatatc gtatgctttt ccaaggtcga
     4141 atgtaaggaa gtcttcgaaa atcccaaaac ccgcattgtt taccagaata tcgacgcgcc
     4201 cgacttcttc catcaccttt tcatataatt cgtccacgct ttcaggattg ctgacgtcta
     4261 ccttgaaagc ataagcctct ttgccgctca agcgtctgca ctcgtctctg acaccttcta
     4321 caaatgccat tcttcgtgcg caacttataa cgattgctcc ttgctttgct gcttcataac
     4381 agatttgtgc gcccagaccg cctgaactac ctgttactaa aacaacttta tcttctaaat
     4441 tcatgcctct tcctcctcgc gaaatggaat ctcaatgcta tcaaaatctt tcataatcgt
     4501 cgtgttttca aacacttctt gtgcttcttt ttcaagctct aaagctgctt ttcctaaata
     4561 tctcgcactg atatgagtaa gtagcagccg ttttgcctgc gcttcttttg caacttcagc
     4621 agcttgatga gtcgtggaat ggaaatagtt gtgcgccatt cttgcttcat ctttattaaa
     4681 tgtgctttca tgtaccagaa tatcgctatt ttccgcaagc acaacggaat tatgtgtttt
     4741 tctcgtatcg cctaaaatag tcacgattcg tcctttttta tctggaccta cgaaatcttt
     4801 tccattgatc gtccgcccat cttctagttg gatcgtttca ccttgcttca gtttgccgta
     4861 cagtggtcca gctggaatac ctaattcttt tagacgatcc acctgcaatt ctcctttatg
     4921 atcatgttca accacacgat aaccaaaact gtcgatccca tgatttagtt tttttgtata
     4981 aacagaaaat tgctgatcag taaaaatcgg ggctgtttca tttagctcaa tgaatttcag
     5041 cggataactt aaacgcgttt gcgaaatacc taaagagact tttatgtatt cctctatccc
     5101 ttttggcccg taaatctcta atggcgtatc cccaccttga aatgaccggc tgctgatgag
     5161 cccgggaagt ccaaaaatat gatcgccgtg taaatgtgta atgaaaattt taccgatttt
     5221 tctcggacga atagtcgttc gcaaaatctg catttgcgtg ccttcgccac agtcgaacaa
     5281 ccaaacttca ttgcgctcgt ctaaaagttt caatgctata cttgtcacat ttctatgttt
     5341 tgcaggaaca cctgcacctg ttcctaaaaa ttgtaattcc ataatctacc tgactttctt
     5401 taagttcctc tttaatttta gggtaaaaag aagggataat caaccgtaag tcgagaatgg
     5461 gttcaaatta aaaataacgt tctaaagcga tagcgttttc tttgaaaatt actataaaaa
     5521 cagcagcgaa cccgtttgag atcctctgct gttttttatt aatttgaaga tccagaactt
     5581 tcattgtcgt tggaagtttg cggatgtttg tccttccaag tcttttgtgc agcttctttc
     5641 gcatgatttt cagcttcttt cgcatcatgc acatcttttt cgacatcttc ataatccaag
     5701 cgagtaattt ctccgcctaa ttccatcatt accaattgat tcagtgggcg attgtcctct
     5761 tcatcagcaa tcaagatcag tcctgtagct ccttcaccga ttttatttcc tacaaagtcg
     5821 aacacggtct gggcatcttt tatttctttg gcatcttgtg tcgcaccgat cattcctccg
     5881 gcaaaccatc ctaataaaat ccctagcgga ccacctaata caccaaccaa cattccgatt
     5941 aatccacccg tagaagattt gttattaccg gtaaagtcga taaaatcttc tattttgaat
     6001 tgatgtgtcc cattaggatc atggctgaca actgccatct gttctccttt aatcgaacga
     6061 acagcatgta actttttgat ttctgaaaat gcctgatatg cctggctttc tacttgaaaa
     6121 tttaaaatga ttactcgttt gctcattaaa aacgcccctt tcataccttc ttgttattta
     6181 ttttaccttt aattgtaaaa taagaaaaga aaagcactct ttttttccaa taaaataggg
     6241 ctgtgacaaa agtcgtatca cagctccttt ttccgaataa acggcgttct tgaagctgat
     6301 cacttctgtc acaacctccc ttaaatctga atctatattt tttggtattt gtttttattt
     6361 gcttgcctat tcgacaaatt caaattcaaa ctcaccgata cgtacaaggt cgccatcttt
     6421 cgcaccgcgt gcccgaagag cttcatccac acccatacca cgtaattgac gagcaaaacg
     6481 catcactgtt tcatcatgat cgaaattcgt catttggaat aatttttcga tcttctcacc
     6541 agataagatc cacgttgcat ctgaatcacg atcgatttgg aactcaggac cttctggatt
     6601 gaatccatag tgtacagttt cttcttccag ttcttcttcg tacaatggga attctggcgt
     6661 tacttctaac aaatctgctg tagcattcaa taacgcgtcc aatccttgtc gagtcactcc
     6721 agaaattgga aatactggga tgtcatcagc aaattcatct tctttttcct tgttcagctg
     6781 ttctttgaat ttcactagat tttccggtgc atccggcata tccattttgt ttgccacgat
     6841 gatttgcgga cgctctagca agcgtaagtt ataagtagat aattctttgt tgatggcaag
     6901 atagtcttca taaggatctc gtccttccat cccactcata tcgatcacat gtaaaattac
     6961 tcgtgttctt tcgatatgac gaaggaattg tgtacccagc ccaacacctt gcgaagctcc
     7021 ttcaatcaag cctggcaagt ctgctgcggc aaaacttctg ccatcacttg tagtaaccat
     7081 tcctagatta ggtacgagcg tcgtgaaatg ataagcgccg atttttggac gagcagatga
     7141 aataacggat agtaaagtcg atttcccgac agatggaaaa cctacaagtc caacgtctgc
     7201 caatactttc aattctagtt cgatttttcg ctcttgtcct ggttcaccgt tttcagcaat
     7261 ttccggtgca ggattccgag gagaggcaaa acggatattc cctcgtccac cgcgtccacc
     7321 ttttgccaca actagtgttt gaccattttc aattaaatct cctaaaagag caccagtctc
     7381 cgcatctcgg acagtggttc cttgtggtac tttaacatag gtatcttctg aaccacgacc
     7441 gtgcatccct ttactcatcc cattttcacc tggctgcgct ttgaaatggc gattgaaccg
     7501 aaaatccatc aatgtacgta acccttcatc gacgatcaag atgacatcgc cgccacgacc
     7561 accgtctccg ccagccggtc cgccgtcagg aacgtatttt tcgcgacgaa atgcaaccat
     7621 tccgtctccg cccttgccag ctttcacatc gatcgttact tgatctaaaa acatggacat
     7681 aattaagtcc tcctattatt catttattta tttgactgcc tgaatttcca caataaacaa
     7741 aagtcttact tattttatcg gtgaaatctg attttgtcta tcatttcagc aaaaagattg
     7801 tttaccttct tcttttttta tcatttggta aggtttccaa ccttttttct gctaccaagt
     7861 ataatttttt gacaaaatat tctttgcgta gcagaataac agtgtattaa ttgtattaat
     7921 tgaggatgcc gtttcacagc acccctaata aaggaggaac ttttgatatg acagtaaaag
     7981 taggtattaa cggatttggg cgtattggcc gtctggcttt ccgccgcatc aaagaagtct
     8041 cagatgatat tgaagtagta gcaatcaacg acttaacaag tccaactatg ttagcacaat
     8101 tattgcaatt tgactctact catggtactt atcctggcac agttactgca acagaagact
     8161 caatcgtagt ggatggcgaa gcaactcgtg tttatgcaga acccgacgca agcaaaatcc
     8221 cttgggcaaa agaaaacggt gtagatatcg tactagaatg tactggtttt tatacatctg
     8281 aagaaaaagc aaaagcgcat ttagatgctg gtgtcaaacg agtagttatt tctgccccag
     8341 ctggtgctat gaaaaccatc gtctataacg tcaatgatga tacattagat gaaaacgaca
     8401 aaatcatctc agcaggttca tgtacaacaa actgtttagc acctatggct tatttcttaa
     8461 ataatgaatt tgggatcgaa gttggtacaa tgacaacagt tcatgcctac acttccacac
     8521 aaatgctctt agatggacct gtaaaaggcg gtaatttgcg tgctgctcgt tcagctgctg
     8581 acaatactat cccacattct actggtgctg cgaaagcaat cggcttagtt atccctgagc
     8641 tacaaggaaa attacaagga cacgcacaac gtgttccagt cgttgatggt tcattaactg
     8701 aattggtttc agtattgaaa acaaaagtaa ctgctgatca agtaaacgaa gcaatcaaaa
     8761 aacacacagt tgacaatcct tcattcgggt atgatgaccg tcaaatcgtt tctagcgatg
     8821 tcattggaac aactcaaggt tcgatctttg acccaacgca aacagaagta acaactgctg
     8881 gtgatttcca acttgttaaa actgttgcat ggtatgacaa tgagtatgga tttacttgcc
     8941 aaatgattcg cttactagaa aaattcgcta acttgtaatt tgatagatca atcgataaaa
     9001 gaagcctttg actggatcca gtcaaaggct tctttctttt catcccctac ttttaaactc
     9061 gttcaatgaa catattggct taaaaattgt tggactactt gctgatattt ttcaggatct
     9121 gtcttgtatg cttccgcatg ctcggctcct tttacaacat acttttcttt tggtcccttc
     9181 gttgcacgat agaccttatc taacatttca aatggtacaa atgtatcttc gtctccatgt
     9241 ataaagagca ttgggcgctg gtttttcttc aattgttcca ctgcatttgc ttcaccgaaa
     9301 aagtagccag cacggacttt tgtcactaga ctcgttactt gcataagcgg aaatgctgga
     9361 agactaaaca tatcgtctag ctgataagca agctcatcat gaacggaaga ataaccgcaa
     9421 tcttcaatga ttgctttcac attatctggt agtttttctc cgctagtcat cataactgtt
     9481 gctgctccca tgctgacacc gtatagaacg atttcttgcg attttccatt gttttctagc
     9541 actttgttta tccactgtac atagtcttta cgctctggcc atccaaatcc aatgtaatcc
     9601 ccttcgcttt ttccatgtcc tcttgcatca ggaacaagga cattgtaacc catatcatga
     9661 aacatctttg cgtaattggt catcgtttca gcgtttccca tatagccatg agccacaaga
     9721 gctgtttttt cacttttcgt ttcagcagga agataaatcg ctgataattt cagaccgtct
     9781 tttgaagtga tggtccaatc gcttcggttt tcttttgaac taaaccattt atgtgcatcc
     9841 gtttctttgg atgtctgtgt tgtaccaggt gtatcttcgt ctaaaaagtc tttctcagaa
     9901 ggcacaacag cataattata aaaataattc cctgcaaaca ctaacgcaac aatcagagct
     9961 aaagcgacgc ctaaaaggat tttcatccaa agtttcataa atcatccatc tcatttctct
    10021 tttttcatat aaccttgaat tagtataatc aaagaatctc tactatgcaa tgttcatttt
    10081 tataac
//
LOCUS       DACEEZ010000091        10018 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20313_2017
            SAMN12639966-rid9879223.denovo.091, whole genome shotgun sequence.
ACCESSION   DACEEZ010000091 DACEEZ010000000
VERSION     DACEEZ010000091.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639966
            Sequence Read Archive: SRR10023158
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10018)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10018)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741683.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741683.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:44:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,016
            CDSs (total)                      :: 2,962
            Genes (coding)                    :: 2,889
            CDSs (with protein)               :: 2,889
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 36 of 73
            Pseudo Genes (incomplete)         :: 29 of 73
            Pseudo Genes (internal stop)      :: 18 of 73
            Pseudo Genes (multiple problems)  :: 8 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10018
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639966-rid9879223.denovo.091"
                     /isolate="E20313_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(25..144)
                     /locus_tag="IWO89_002383"
     CDS             complement(25..144)
                     /locus_tag="IWO89_002383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3789 domain-containing protein"
                     /protein_id="HAQ0457100.1"
                     /translation="MEIVKDILLVFGGSMLGVTVMCLMHASAAADRAMEKKGK"
     gene            complement(159..1343)
                     /locus_tag="IWO89_002384"
     CDS             complement(159..1343)
                     /locus_tag="IWO89_002384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HAQ0457101.1"
                     /translation="MAQRNLDYRLLKDRRNEYGVSQNKLATTCGLSRPYLNQIENGGV
                     TASTKTMRKIFDQLESFNPELPLTLLFDYVRIRFPTTDARKIIQEILHLKFDYMLHED
                     YAFYSYQEQYVMGDIVVMLSHEEDKGVLLELKGRGCRQFETFLLAQKRSWYDFFEDCL
                     KAGGVMKRLDLAINDRVGLLDIPDLTKKCQKEECISLFRTFKSYRSGELLKPDDKDGM
                     GNTLYIGSLKSEVYFCLYEKDYEQYIKLGIPLDQTETKNRFEIRLKNDRAYHAIQDLL
                     KGRSIESTTFSIINRYLRFADKVEGKRRTNWPLNEQWGRFIGRNQKEIQLTSEPKPYT
                     IERTLNWLGRQVAPTWKMAKELDRLNQTTYIQDMVNNTRLSDRHKKILEQQSMAIENL
                     II"
     gene            complement(1599..1853)
                     /locus_tag="IWO89_002385"
     CDS             complement(1599..1853)
                     /locus_tag="IWO89_002385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0457102.1"
                     /translation="MIGKIQSDPELEEELYRLNFDIRKNGVKVPDDPYVIDEETDARM
                     ELNQVISELERIADFAKEPDIRQYLFEIKAELEIEGITAE"
     gene            complement(1878..3020)
                     /locus_tag="IWO89_002386"
     CDS             complement(1878..3020)
                     /locus_tag="IWO89_002386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016613853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA cytosine methyltransferase"
                     /protein_id="HAQ0457103.1"
                     /translation="MTFLDLFAGIGGFCLGMEQAGHQCIGFCEIDDFARQSYKAIHDT
                     SKEVEMHDITSVSDEFIQSLGPVDILCGGFPCQAFSIAGKRQGFSDTRGTLFFEIARF
                     AAILQPKFLFLENVRGLLNHEGGATFETILRTLDGLGYDVEWQVLNSKAYVPQNRERI
                     FLIGHSRDACTEQVFPIIGSSPTSDQNIRNLLNINPSNRGMGGQVYGSDDVAPTLTGD
                     EGIKIALPVDDGISVAGMLLGNFEQGNRVYEITGTAPTLSTKQGGTKIMLRKNETEYQ
                     EVKPGNSVNLAFPNSTSRRGRLGKQSVHTLLTGDQQAVVTDQYQIRKLTPRECWRLQG
                     FPDWAFDRASQVNSDSQLYKQAGNSVTVPVIFDIARRLKEVKTDDL"
     gene            complement(3080..4432)
                     /locus_tag="IWO89_002387"
     CDS             complement(3080..4432)
                     /locus_tag="IWO89_002387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016613852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF87 domain-containing protein"
                     /protein_id="HAQ0457104.1"
                     /translation="MYKGHRIRAGDQHLVYHFVLGWLLALFIGWMSVFYFQELRQFDI
                     SKLSLSTIEIVRSIKDLIYLLGSLVLSGSTMLLYIHFFQDHWRSLWHRQKLARMILEN
                     HWYEVKQTQSEGFFKDLNSSRTKETISYFPKIYYRMKDGLLSIRVQISLGKYQDQLLK
                     LEKKLESGLYCELVEKELKDSYVEYTLLYDIIANRIGIDEVVAESGALRLMKNQVWAY
                     DSLPHMLIAGGTGGGKTYFLLTIIEALLKSDAELFVLDPKNADLADLGTVMPHVYSQK
                     EEISACVEDFYERMMTRSKAMKEMPNYKTGENYAFLGLPPNFLIFDEYVVYMEMLTTK
                     ESAVILNKLKQIVMLGRQSGFFLILACQRPDAKYLGDGIRDQFNFRVALGRMSELGYS
                     MMFGEVDKKFFMKRIKGRGYVDTGGSVISEFYTPLVPKGYDFLREISDIGGLNVNTEG
                     "
     gene            complement(4503..4847)
                     /locus_tag="IWO89_002388"
     CDS             complement(4503..4847)
                     /locus_tag="IWO89_002388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="damage-inducible protein J"
                     /protein_id="HAQ0457105.1"
                     /translation="MEINVEHILDCLDQYGKGELTEEQLTKALTYDEKMFLIMHQGLL
                     EVGNDAEEDFDVLNWLGEEESFFMVIEVNESLCREAETVLEEIGVEMPDAIEVFLKQL
                     VETKQLPIAVND"
     gene            complement(4857..5231)
                     /locus_tag="IWO89_002389"
     CDS             complement(4857..5231)
                     /locus_tag="IWO89_002389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HAQ0457106.1"
                     /translation="MRLTEGIVVDSGLTFGKLRFSALRREVRKQNEDGTVSNEVKERT
                     YNLKSSAQGRMIQVSIPANVPLREFAYDAEVELVNPIMDTVANYVFREGTTVNWFIKA
                     DDLVLKRQPDQGNSTNQNEGKK"
     gene            complement(5244..5558)
                     /locus_tag="IWO89_002390"
     CDS             complement(5244..5558)
                     /locus_tag="IWO89_002390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HAQ0457107.1"
                     /translation="MELKFVVPDMAETFGKIRYAGEGEVLTEGYGRNTTVIGRSYHLY
                     SSKQRADDIEVVVAAEAGEKDFDQDQPLKAVNPHLVAKGYEIENRGFTDYVLYVDDLV
                     KA"
     gene            complement(5672..8897)
                     /gene="fss3"
                     /locus_tag="IWO89_002391"
                     /pseudo
     CDS             complement(5672..8897)
                     /gene="fss3"
                     /locus_tag="IWO89_002391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286924.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fibrinogen-binding MSCRAMM adhesin Fss3"
     gene            complement(8976..9638)
                     /locus_tag="IWO89_002392"
     CDS             complement(8976..9638)
                     /locus_tag="IWO89_002392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010740290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0457108.1"
                     /translation="MKQNDGRIIWKNQSELKLILTINEFIEKHGITSSRQYQKKLSEN
                     PNSAPSMWFINKKYGSWENLLISIGRENTGYGKWARMSEQELLEIVEAFIKCEKITSQ
                     RMYEQKSVGKNIPSLSTIKKMLGDIRPLFKEKNDGSRFTDFELLLELKNEIIRLKLQD
                     DLSMTKFRKLVQSPKLPSVDTIMKRTNKNWEELMAEIGFDYRRIKIYKQRNNLSKTKK
                     TK"
BASE COUNT         2701 a         2262 c         1710 g         3345 t
ORIGIN      
        1 aaattttgac cgaaattcat ttcatcactt tcctttcttt tccattgctc ggtccgccgc
       61 tgcacttgca tgcatcaagc acatcacagt aacaccaagc atactgccac caaacaccaa
      121 taaaatatct ttcacaattt ccatcacgat tcctcttttc atataatcaa attttcaatt
      181 gccatacttt gttgctccaa aatctttttg tgccggtctg atagtcgagt attatttacc
      241 atatcctgaa tgtacgttgt ctgattcaac cgatccaact ccttggccat cttccacgta
      301 ggggcgactt gtcgtccaag ccagttcaac gttcgttcaa tggtataagg cttcggttca
      361 gaagttaatt gaatttcctt ctgattccgt ccaatgaagc gcccccattg ttcattcaag
      421 ggccagttag ttcgcctttt gccttccacc ttatccgcaa atcgcaagta ccggttaata
      481 atggaaaatg tggtactttc aatactgcga ccttttaaca aatcttgaat tgcatgataa
      541 gctcgatcat ttttcagtcg gatttcaaaa cgatttttcg tttcggtctg gtctaaagga
      601 atccctaact tgatatattg ctcatagtct ttttcgtaca aacagaaata gacctcactc
      661 ttaagactgc caatatacaa agtattgccc ataccatctt tatcatcagg tttcaacagt
      721 tccccggaac gatagctttt gaaggtacga aacaaggaga tacattcttc cttctgacat
      781 tttttcgtta aatcaggaat atctaacagg ccaactcgat cattgattgc caggtctaag
      841 cgtttcatca cgccaccggc ttttagacaa tcttcgaaaa agtcatacca gctacgcttt
      901 tgggcgagta aaaaagtttc aaactgccga caaccacgac cctttaattc taaaaggacg
      961 cctttatctt cttcatgtga caacatcacc acaatatctc ccataacata ttgttcttga
     1021 taggagtaaa aggcgtaatc ttcgtggagc atataatcaa atttcaaatg gagaatctct
     1081 tgaatgattt tcctcgcatc cgttgtggga aagcgaatcc ttacatagtc aaacagtaac
     1141 gttaaaggta actcgggatt gaaactttcc agttggtcaa aaattttcct catggttttt
     1201 gtcgatgcgg ttacgccacc attttctatc tgatttaagt acgggcgact aagtccacaa
     1261 gtcgtagcta atttattttg tgaaacgcca tattcattac gccggtcttt taatagccga
     1321 taatctaaat tcctttgtgc caagttctca cctccaaatt taaggtgtta actttttcaa
     1381 atcagttaac atctacacaa attgcttatt atccttgata ggacagccat tccttatcgt
     1441 tgcttgctta ttttttgtgt ttttgtaccc ccctgttaga tacggggggt gagacatcat
     1501 ggccggctaa cgccgtccat aaacaggcac agcaccacgg aggatttctg cttcgcaaat
     1561 cccccgcggt cagtgcctat cagtggttta tttggccgtt actccgcggt aataccctca
     1621 atttccaact ctgcttttat ctcaaacaag tattggcgga tatcaggttc tttagcaaag
     1681 tccgcaattc gttccaattc actgatgact tgattcagct ccattcgagc atccgtttcc
     1741 tcatcaatta catacggatc atcagggact ttcaccccgt ttttgcgaat atcaaaattg
     1801 agccggtaca gttcttcttc caattccgga tctgactgaa tcttgccaat cacccagtag
     1861 attccccaat caactcatca taaatcatct gtttttacct cctttaatct tctagcaata
     1921 tcaaaaatga ccggaactgt tacagaattt ccagcctgct tatacaactg actatctgaa
     1981 ttgacttgag aagctcgatc aaaagcccag tccggaaagc cctgaagccg ccagcactcc
     2041 ctaggtgtta actttcgaat ttgatactga tccgtgacga ctgcttgttg gtctcctgtt
     2101 aataaggtat gcacactttg ttttcccaga cggcctcgac gacttgtgga attaggaaaa
     2161 gccaggttca cactgttccc tggcttcacc tcttgatatt ctgtttcgtt tttccgaagc
     2221 atgatcttcg ttccgccttg tttcgttgac aaagtaggcg ctgttccagt tatctcataa
     2281 acccgattgc cttgttcaaa atttcccagt aacatacccg ctacagaaat tccatcatct
     2341 accggcaatg cgattttgat tccttcatca ccggtcaagg taggcgctac gtcatctgac
     2401 ccatagactt gtccccccat tcctcgattg gaagggttga tatttaataa gtttctgatg
     2461 ttttgatcag atgttggaga cgatccaatg ataggaaata cttgttcggt acatgcgtct
     2521 cgagaatgtc cgataaggaa gatccgctca cggttttggg gaacgtaggc ctttgagtta
     2581 agcacttgcc attccacatc ataccccaat ccatccagcg ttcggaggat cgtctcgaac
     2641 gtagcccctc cttcgtgatt gagtaatccc cggacgttct caaggaataa aaacttaggt
     2701 tggagaatag cggcgaaccg agcgatttca aagaataaag tacctcgagt atcggaaaat
     2761 ccttgccgct ttcccgcaat tgaaaaagct tggcacggaa atccaccgca aaggatatcc
     2821 actggtccga gagattgaat aaactcgtct gatacacttg tgatgtcatg catttccacc
     2881 tccttacttg tatcatggat tgccttataa ctctggcgag caaaatcatc aatttcacaa
     2941 aagccgatac actggtgacc cgcttgttcc atacctaagc aaaagccacc aatcccagcg
     3001 aataaatcta aaaatgtcat aaatcacctc gtaaaaaaag cccaggattc ttgacactcc
     3061 taggctacat actattattt tatccttccg tattaacgtt taatccacca atgtcgctaa
     3121 tttcccttaa gaaatcatat ccttttggta caagtggcgt ataaaactca ctaatcacac
     3181 tccctcccgt atctacatag cctcgacctt tgatacgctt cataaagaat tttttatcaa
     3241 cctcgccaaa catcatggaa tatcccagtt cactcatacg acccaaagcc acccggaaat
     3301 taaattgatc gcgaatcccg tctcctaaat atttcgcatc cggtctttga caagctagaa
     3361 tcagaaagaa accagattga cgacctaaca tcacgatttg tttgagctta ttcaaaatca
     3421 ccgcactttc cttcgtcgtt aacatttcca tataaaccac gtattcatca aagattaaaa
     3481 agtttggtgg aagtccaaga aacgcataat tctctcctgt tttgtagttg ggcatttctt
     3541 tcattgcctt gctacgagtc atcatgcgct catagaaatc ttccacacaa gcagaaattt
     3601 cctccttttg ggaatagaca tgaggcatta ccgtacctaa atcggctaag tccgcatttt
     3661 tgggatcaag aacaaaaagc tcggcatcag attttaataa agcctcgatg atagtgagta
     3721 aaaagtaagt cttcccacca cctgtgccgc cagcgattaa catatgaggt aaagaatcat
     3781 aagcccatac ttgatttttc atcaatcgca aagccccact ttctgccact acttcgtcta
     3841 tcccaattcg attggcaatt atatcataca gtaaagtgta ctccacatag gagtctttga
     3901 gttccttttc tactagttca caatatagac cactttctaa cttcttttcc agctttaaaa
     3961 gctgatcttg atattttccc agtgaaattt gaacgcgaat agaaagtaaa ccatccttca
     4021 tacggtaata gattttgggg aagtaactga ttgtctcttt ggttcgacta ctattgaggt
     4081 ctttgaaaaa tccttcactc tgagtttgtt ttacttcgta ccaatgattt tctagaatca
     4141 tccgggctag cttttgtcga tgccacaaac tgcgccaatg atcctgaaaa aagtgtatat
     4201 aaagcaacat cgtgcttcct gaaagaacga gacttcctag caaatagatt aaatctttta
     4261 tagaacgaac gatttctata gtagaaagtg atagcttaga aatatcaaat tgtctaagct
     4321 cttgaaaata aaagacgctc atccaaccga tgaacagagc gagtagccag ccaagaacaa
     4381 agtgataaac caagtgctga tctcctgccc gtatacgatg ccctttgtac atttttatgg
     4441 tttccacctc caatcgaaaa aagcttcata ggatttcatt gatccaattt caagcagtta
     4501 tgctagtcat ttactgctat agggagttgt ttcgtttcca ctagttgctt taaaaaaact
     4561 tcaatggcat ccggcatttc cacaccaatt tcttccaata cggtttcggc ttctcgacat
     4621 agactttcat ttacttcgat aaccataaag aaggactctt cttcccctaa ccaattcagt
     4681 acatcaaaat cttcttcggc atcgttacct acttccagta acccttgatg cataatcaga
     4741 aacatttttt catcataggt taatgcttta gtgagttgtt cctccgttag ctcacctttt
     4801 ccatattgat cgagacaatc taaaatatgt tcaacattaa tttccatacg cttcacctat
     4861 ttctttcctt cattttggtt tgtagaattt ccctgatctg gttgtcgttt caagactaaa
     4921 tcatcggcct taatgaacca attgacagta gttccttctc ggaaaacata attggcgacc
     4981 gtatccatga ttggattgac caactctacc tctgcgtcat aggcaaactc gcgtaagggt
     5041 acatttgctg ggatactaac ttggatcatt cgaccttgcg cagaggattt caaattataa
     5101 gtccgttctt ttacctcgtt actaaccgtt ccatcctcat tttgcttccg tacttctcgg
     5161 cgtaatgcgg aaaatcgtaa tttcccaaac gttagtcctg aatctacaac aataccttct
     5221 gttaatctca tcttcttttc ctcctatgct ttcactaaat catcgacata caacacgtag
     5281 tcggtaaacc cacgattctc aatctcatag cctttggcaa ccaaatgggg attcacggct
     5341 tttagtggtt gatcttgatc aaaatccttt tcgccggctt ccgcagctac caccacttca
     5401 atatcatcgg ctcgttgctt actggaatac aaatgataac tacgaccaat tactgtagta
     5461 ttccgcccat atccttcagt taaaacttcg ccttcgccgg cataccttat ctttccaaag
     5521 gtttcggcca tatctggcac aacaaatttc aattccatct tcttttcctc ttttcatttt
     5581 tagttttgga tacaaaaata gcgcgaccac tttttttagg aggcggtcgc gcacgttggt
     5641 ttttcctatt tagttgtttc atcacatcac cttagtttcg gcgattccag aaataatacc
     5701 cagcagtcgc aaagatcaag gtaaacccaa taaacagcag aacattagaa tttttctccc
     5761 ctgtttgtgg gaatgtcttt actgtaggac tagattcttg agagtcactt ggaacagttg
     5821 gtatttccgg ttgttctggt gcacttggtg ttgtcggaac ttttttaggt gttaaagttt
     5881 gagttttttc ttttagatcc tcattgtgtt tcccattgat gtttccgtct ttgccatagt
     5941 taatttcttt gaaggtaaaa cttgtacctt ctggatattt acttgtgtcc actttttccg
     6001 caagtacggt tttagtgaat tctttgtcat tcacttcata atcaattttg ccagatttcc
     6061 aaatttcttt ggtcgtatta tccggaagta atgcatacag aattgtttca aaagcttctt
     6121 ttgagccatc cagtacatca tgggtaatcg acacatcatc gaacatatcc accacatccc
     6181 catgagtaaa ggtttgcgaa ccatcttcta ggtgggcttt tgtttggatg gaaacatggc
     6241 gttccaccgt acaattgaca gtttgggctt gattgttcaa gctagcatct ttcgctactg
     6301 gatcatcacc tgcttcatag ggtttcttgt tttcatacac atagttgaat aagacgatgg
     6361 ttttatccaa aacttgttct gcggttaatt tatgcaaaat tttccaagtt cctgttttct
     6421 ctttattcgc caacgttgcg gttgtttcgg caattaccac tggtttggct ttttcatctt
     6481 tttcttgggc agcttttgtc gcttccacat caatggcttg cgctaccaca taccagtctt
     6541 ctttgatttc atgcaagtta tagctcaatt tatcaactag ctcggtagaa tcaaggtctg
     6601 tcaattcttt gcctccgtct ttccaagtcc caagagaagt taagctatct tcttcttcag
     6661 gtaaatcata gagcatcaaa cgattcaagc taacagaaaa tgctttattc gtcagtttct
     6721 cgtaaggaac ggtcaccgtt ttaattggtt gtttttgtcc tttttcggta atcgtaaaaa
     6781 tgtattcact cttagtgtaa tccgctttat tttctttaaa agtagaacgg atttctaatg
     6841 gcgtaatttt ttgaaatcct tctggagcct ctacttcttc aagtaaataa tccccataag
     6901 gaagattttc aaacttgcca tagccatcaa aacctagttg ctcgttataa gctgtaattg
     6961 cttcatcttc ggcacctgta atctcactgg ttccttccaa tggcgatact ttaaaggtca
     7021 gatcattgaa gccagtttcg gcagtcccag cagctgatcc agcaaattta aagaaatcaa
     7081 agccaaagcg aatgacttgt tcttttgccg taacatctcg ggtaatcacg gcattttttt
     7141 cgttatcatc cacctttttg atggaaacag ggtactttgt ttcatccaag gtatatcctt
     7201 caggtgcttt ggtttcttgc cagtaatact cattaatcgc taagtgttta acagcaactt
     7261 gattcttttc atctaaagtc aaagtcaccg tttcatcaga agctttcgtt cccttcacta
     7321 actctggttt aaaagcctca ctccatttga ccgcttgacc atcttttgca gtaaagagag
     7381 tatattcagc tcctttaaac tcagctttac cttgagtttc attaccggta tctttgtctt
     7441 cttttgtcaa agtggtttca ccagtgattt cttggttttg cccttttacg ttactggtaa
     7501 caagagccac ggtttgattg acatagttca attcgacttt tactggttta aaggtattca
     7561 caaagccatt actagctttg gtttcggtca cataataggt tcccaattcc aacgcattgg
     7621 caatctcttt tggtgtttcc gcatgacctt tttcatccgt ggtcatttct tggacaattt
     7681 caccggttgg actgtctttg cgaatggcaa acgtatttcc ggctaatgag tatttgtcgt
     7741 tccaaagatc agttcctgtt tccaccccca ttttattcaa aatgatttga cctttttcgc
     7801 gagtattttt tgaagtcaca gaaatagtct caccagattt aatggttgcg gtgattggtg
     7861 tagtgtcaat cgtataagga gctggcactg atttttcagt aatggttact ttcgcgccat
     7921 gaggaatctc atccaatgtc gcaatgcctt ctttatcggt ggtcacgtct tttgcaggta
     7981 atgtttttcc aaagtctaaa tggaaaacgg ttcccggtac aacatttcct gtttctttat
     8041 cgactttttt aatcttcaaa gaaccctcag tttccacatc aattttgatt gtgtaggtat
     8101 tgggcttatc aattgccccc gccatcaaag tttgttgacc agccatttta taagctaccg
     8161 gtgttcctgt attagctgac ttctttaggg ttaattcacc actctctttt gaattggcat
     8221 ttggagtaat cactagttga ttgccgttca cacgataatc aatatttgcg atattctcga
     8281 caacttcatc aaactccgat aaattcaagc cgttcgtatc tgtcacagta gtcgattgac
     8341 ctaacacggt tttcactgtg gagttatgga agcttggttt cttttgataa tccgcaatgg
     8401 cttgattgat ttttgactca atggctgcga tatttactgc actaccatca gaacgtttaa
     8461 ttgagtgaag cgtagccatt aacttcttgc caaatcatct tctgggccac aatatgtgta
     8521 tccacatccg taccagcttc cttccataaa acggaaacta atttcgcttt ctccgacata
     8581 tcaggcaatg gatttttctc ataaccagga gtgaactcat tgtaaatcgg cacccctggc
     8641 tcaatacaga aaatatcttg cttcacacca ttgtaaactg catagtgcgg aacggtaact
     8701 tctgacatgg gacgaccatc gctaaatgta ccttcgacag catactggtg agccaaatcc
     8761 aattccacag ttaccgtttg tggatgagta atttcatcag caaaggcaat cgttgggcca
     8821 agcgatccac caattgtttg gcataataga ctcagtgttg cgagcaaacg ccaattctta
     8881 aatcttggct ttttcataaa ataaatctcc tttcgttttt gggtaataaa aaagacgtcc
     8941 aaattttaat tttgaacgtc tgatttagag tgatactatt tagttttctt tgtcttggat
     9001 aggttgtttc tctgcttata aattttgatt cttcgataat caaatccaat ctctgccatc
     9061 aattcctccc aatttttgtt cgttcttttc ataattgtat caactgatgg tagttttggg
     9121 gattgaacta gttttcgaaa ctttgtcatt gataaatcat cctgtagttt taatcggatt
     9181 atttcatttt ttagttctaa cagcagttcg aaatcagtaa atcgagatcc atcatttttt
     9241 tctttaaaaa gtggacgtat atctcctaac atctttttaa tggtactaag tgaaggaata
     9301 ttcttcccta ctgatttttg ttcgtacatt cgttgagagg ttatcttctc gcacttaata
     9361 aaggcttcta ctatttccaa taattcttgt tctgacattc tagcccattt tccataccca
     9421 gtattttctc gtcctatact aatcaataaa ttctcccatg atccatattt tttgttaatg
     9481 aaccacatac ttggtgcaga atttggattt tctgatagct ttttctggta ttgacggcta
     9541 gatgtaattc cgtgtttttc gatgaattca ttaattgtta aaatcagttt taactcactt
     9601 tgatttttcc aaatgatacg tccatcattt tgcttcacga taaaaactcc taatagttat
     9661 ttagatagaa gcctatcaca tcaacttttt agtagaaaag tacatgtttt gtcttttttg
     9721 ctaatagctg accctttgag aaacttgggt tcaaaagttg ctaaattcgg taaatgtttt
     9781 ggtatttttc gtattatatt tgtcgtcaag ctgactacta aattttgccc tattttgacg
     9841 acactgatag tcaatgtcgg acaatctagg acaattttta agggaatatg attgaaaaat
     9901 ggtccaaaac agccaaaaat ggcttaaaac tgtgatagag aaaaaagaaa aagcccttga
     9961 tatacaagga cttttcacaa atttcagatt tccaaggaag ttttccgtgt ttatcttc
//
LOCUS       DACEEZ010000092         9770 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20313_2017
            SAMN12639966-rid9879223.denovo.092, whole genome shotgun sequence.
ACCESSION   DACEEZ010000092 DACEEZ010000000
VERSION     DACEEZ010000092.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639966
            Sequence Read Archive: SRR10023158
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9770)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9770)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741683.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741683.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:44:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,016
            CDSs (total)                      :: 2,962
            Genes (coding)                    :: 2,889
            CDSs (with protein)               :: 2,889
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 36 of 73
            Pseudo Genes (incomplete)         :: 29 of 73
            Pseudo Genes (internal stop)      :: 18 of 73
            Pseudo Genes (multiple problems)  :: 8 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9770
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639966-rid9879223.denovo.092"
                     /isolate="E20313_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            <1..321
                     /locus_tag="IWO89_002393"
     CDS             <1..321
                     /locus_tag="IWO89_002393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter ATP-binding protein"
                     /protein_id="HAQ0457109.1"
                     /translation="ETIIAHSAFADQIANKWLVELSYIGNQTNEPLIAHLYSKYQVTA
                     NILYGNVELLQETPLGSLIVTLAGETKQRKKALDYLIKSGVKINILQKNDLDSRIKVI
                     EGGR"
     gene            323..1003
                     /locus_tag="IWO89_002394"
     CDS             323..1003
                     /locus_tag="IWO89_002394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAQ0457110.1"
                     /translation="MTDFFKTYLPNVYLIPDEFVEATKQTLYMSFWTAFIGGMIGIIL
                     GVTLVVTRPNGLLANRLLFEILDKLINIIRSIPFIILLSLLALTTRFLVGTTIGAKAA
                     LVPLIFGVVPFFARQIENALLEVDPGVIEAAEAMGTSPIGIILRVYLLEGLPGIIRVS
                     ALTIINVIGLTTMAGAVGAGGLGNLAISRGYNRFQTDVTIVATLLILILVFLSQFISN
                     QLIKKITH"
     gene            1020..1859
                     /locus_tag="IWO89_002395"
     CDS             1020..1859
                     /locus_tag="IWO89_002395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MetQ/NlpA family ABC transporter
                     substrate-binding protein"
                     /protein_id="HAQ0457111.1"
                     /translation="MKKIIRFVVITFVAIIWLAGCSQGKADTTDKKETIVKLGIIGED
                     TDVWDNVKDRLKKENINLEYVKFTDYNQPNVALADGSIDLNSFQHQFFLDNYNKEHGT
                     DLVSIGNTVNAPLGIYSNTLKDVADIKDGAKVAIPNDVTNGGRALLLLQTAGLIKVDK
                     AKGQAPTVSDITENRKNLDISELDASQTARALSDVDISVINSGVAVDAGFNPASDAIF
                     LEPVDDHARPYVNIIVARKEDENNKTYQKIVDAYQTEDTVKVIEKTSKGSSVPAWETF
                     GRK"
     gene            1890..3113
                     /locus_tag="IWO89_002396"
     CDS             1890..3113
                     /locus_tag="IWO89_002396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HAQ0457112.1"
                     /translation="MTTTISTNQLKDLIQYRLPEYQALALDIHEHPEVSNYEFYSSAA
                     LIKQLEKERFWVEKEVAGHRTAFDARYISNKPGPTIAFLAEFDALPGIGHACGHNLFG
                     TYSVLAASIVKQFIDETGGEIRVYGTPGEEGGENGSAKGSFVREGFFEDVDVALCVHP
                     AFRYGKTTESLANDPVDIKFYGVASHAAAAPEKGVNALEALIQVFNGINALRLQLPKD
                     VNIHGIITDGGVAANVIPDYAAGRFYLRAGNRQTLDKVYEKVENIVRGAAISTGTTFE
                     FGLFQNAVDDVIVTPSFDDLFFQHAKEAGVPDEEIETEQRTSLGSSDVGNVSQVIPTI
                     QPTVAISETYIAGHTEEFKAAAKSDKGLASIAIAAELLANTALDLLKEPKLLERIKQE
                     HQEIVARKKQEGATF"
     gene            3516..4934
                     /gene="nhaC"
                     /locus_tag="IWO89_002397"
     CDS             3516..4934
                     /gene="nhaC"
                     /locus_tag="IWO89_002397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaC"
                     /protein_id="HAQ0457113.1"
                     /translation="MGKKGTPSFLEATLVLMVIVLGIAIGVVGLKVSPNITILAAIGM
                     VMLYAVIKRYPTDWLHEGIINGIKPGIIPIFIFILVGSLIAVWIQAGIIPTLMVIGFK
                     LISVQWFVPSVFVVCAIVGSAVGSAFTVMSTIGIAFFGIGTTLGLNPALVVGAIVSGA
                     VFGDKMSPLSESTNLAAAIVDADLFKHIKHMMWSTVPAFVVSFFLFMILGHTSKAASL
                     SSIREVTDVLEANFTISIWSLIPLFLMLLCAWKKVPAILTILLNIAVAVGMIFIQNPQ
                     TSLTKLAGVIESGFVSNTGNQQIDSLLSRGGIESMMPTVSLIILTLSLGGLLIEFGLI
                     STVMERVSRKMTNTPNLIFTTLMTSIGVNIFIGEQFLSVILPGNAFKETYEKAGFDPT
                     VLGRTLEDGGTVINYLVPWGIAGSFVASTFGIPTLTYLPFVFFSLLSPVFSMVSAFTG
                     WGISKKFVKPQEAVSKTAQLEQ"
     gene            complement(5438..5881)
                     /locus_tag="IWO89_002398"
     CDS             complement(5438..5881)
                     /locus_tag="IWO89_002398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF305 domain-containing protein"
                     /protein_id="HAQ0457114.1"
                     /translation="MKKYWKFLLMIGVSTVVMFGMMYLNVYALDHIFFSRTRVFMALM
                     MGAVMAVIMLLFMWKMYENKKINVMILAASLLVFAGSLFMVRSQTTVNDTAWMKAMIP
                     HHSIAILASKRADLKDPEVKKLAEEIIQAQEEEIDKMKKMIKEME"
     gene            complement(6099..7439)
                     /locus_tag="IWO89_002399"
     CDS             complement(6099..7439)
                     /locus_tag="IWO89_002399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAQ0457115.1"
                     /translation="MEDKKLKKQLSSRHITMLALGGAIGAGLFKGSGEAIGIAGPSVL
                     IAFLIGGLILYIVMKGLGKIVLSGGDTHHGLSGLIRPYLGAHSADFTDWVYWSMWIIN
                     IIAEAVAAASFLQLWFPHIPAWVFVFMLAVLTTIINLYSVRLFAETEYWLAFAKISVI
                     ILLIIFATYLVGQQMLGTGVFPTLQQLTDHGGFTPHGMKGIISSLLVVIYSYGGSELI
                     AITVSEADDPKRAIPKAIKGVMGRIISFYIIPLFLLLILFPWNTLAGTNVSPFVMVFE
                     KMNIPFAADIVNFVIVLALFSSINSGVYASSRILFFRLKDRKGSSRKLAVLNKHQVPQ
                     RAVLFCTSVLYLGVALSYFVGDKLFGYLAGSLSYTVLLIWIIISAASFVLALRKGTLW
                     DKGISFFALAVLGLIFLGILFTNSIGVTVLTGLLYLFIYFSYQKKNDAFVLTNE"
     gene            7708..8604
                     /locus_tag="IWO89_002400"
     CDS             7708..8604
                     /locus_tag="IWO89_002400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide biosynthesis protein Alg14"
                     /protein_id="HAQ0457116.1"
                     /translation="MNKNDVLLVAKNKSLYFLPSIRSLDVWSGKDLLQSEFIKSARQQ
                     LANYQLIVFLDYGFDMEMARRVRPFTNAKIVLFFWNHFKAEHEAMLKQAQDEPAIDEI
                     YHFDFLEAKELGLKHNSSFYSKHMNFSSSEPKTDLFFGATNNGRKERAESYKQEFIKR
                     DVTTNYFILPHRGNEQPGYLTYEEYLKKTGESRGILELLREGQQGVTLRTFESMYFQK
                     KLVTDNQAIAHYHFYRPENIFLLRERDLDELPEFLHTPFKPILSELLEFYDAENWAKR
                     FITNDSTIYEQYEYDLSSVENK"
     gene            complement(8754..9695)
                     /locus_tag="IWO89_002401"
     CDS             complement(8754..9695)
                     /locus_tag="IWO89_002401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HAQ0457117.1"
                     /translation="MKIAIAGAGAMGSRIGLMLHQSGNEVLLIDRWPAHIEAIRTNGL
                     IADFNGKEVVAKLPIYSPEEIIESNEHVDLIVALTKANQLDDMFCSIQSIITDNTYVL
                     CLLNGLGHEDVLEKYVPKKNILFGITMWTAGLAGPGKVTLLGDGEIELENLEPEGEAF
                     TKKVVEVFQEANLNPIYSHNVRYSIWRKACVNGTLNGLCTILDCNIAELGAQKAAESM
                     VRTIVSEFASIAAKEGIILDQEEVYQHIASTYDPDNIGLHYPSMYQDLIKNHRLTEID
                     YINGAIWRKGQKYDIATPYCAFLTQLVHAKEGILGAE"
BASE COUNT         3143 a         1805 c         1916 g         2906 t
ORIGIN      
        1 gaaacgatca ttgcccattc tgcttttgcc gatcagatcg ccaataaatg gctggtggaa
       61 ttaagttata ttggaaatca gacgaacgaa cctctaatcg ctcatctgta cagcaaatat
      121 caagtaacag cgaatatttt atatggcaat gtagagttat tacaagaaac gccgttaggg
      181 agtttgatcg ttactttagc tggagaaacc aaacagcgga aaaaagcatt ggattatttg
      241 atcaaatctg gtgtaaaaat aaatatttta caaaaaaacg atttagatag tcgtatcaaa
      301 gtgatcgaag gaggaagata aaatgactga tttctttaaa acatatttgc ccaatgttta
      361 tttgattccg gatgaatttg tcgaagcgac gaagcaaaca ctatatatgt ctttttggac
      421 agcattcatt ggcgggatga ttggaatcat cttaggtgtg acgttagtag tgacacgccc
      481 taatggatta ctagctaacc gtctgctatt tgaaatactt gataagctga tcaatatcat
      541 tcgttccatc cctttcatta tcttgctttc tcttttagca ttgactactc gtttcttagt
      601 tggaacgacg attggtgcga aagcagcttt agtgccattg atttttggag ttgttccgtt
      661 ttttgctcgt caaatcgaga acgcactgct agaagttgat cctggggtga tcgaagcggc
      721 agaagcgatg ggaaccagtc caatcggtat cattttacgt gtgtatttgt tagaaggttt
      781 gcctggtatc attcgggtct cggcgttgac tatcatcaac gtgatcggac tgactaccat
      841 ggctggagca gtaggtgcag gcggattagg gaatcttgct atatcgagag ggtataatcg
      901 attccaaaca gatgtaacga ttgtggctac attattgatt ctaatcttag tttttttaag
      961 tcaatttatc agtaatcaac tgattaaaaa aattacacat tgaaagtgga ggagaaaaaa
     1021 tgaaaaaaat tattcgtttt gtagtcatta catttgtagc aattatttgg ttggcagggt
     1081 gtagccaagg aaaagcagat acaactgaca aaaaagaaac tatcgtaaaa ctagggatta
     1141 ttggagaaga tacagatgta tgggacaacg tgaaagaccg tttgaaaaag gagaacatta
     1201 atttggaata cgtgaaattt acagattaca atcaaccaaa tgtggcttta gcagatggat
     1261 cgattgattt gaattctttc caacatcaat tctttttaga caattacaat aaagaacacg
     1321 gaactgattt agtatcgatc ggaaatactg tcaatgcacc gctgggaatt tattctaaca
     1381 ctttgaagga tgtagctgat atcaaagacg gagcaaaagt agcaatccct aatgatgtta
     1441 caaatggcgg cagagctctt ctgttattgc aaactgctgg attgatcaaa gtggataagg
     1501 caaaaggaca agcgcccact gtttctgata ttaccgagaa tagaaaaaat ctagacattt
     1561 ctgaattaga tgcttcccaa acagcacgtg ctttgtcaga tgtagacatt tcagtgatca
     1621 atagcggtgt agctgtagat gctggattta atcctgcttc tgatgccatc ttcttagaac
     1681 cagtggatga ccatgcacgt ccttatgtga atatcatcgt tgcaagaaaa gaagatgaaa
     1741 acaataaaac gtatcaaaaa atagtagacg cttatcaaac agaagacaca gtcaaagtaa
     1801 tcgagaaaac atcaaaagga tcaagcgtgc ctgcatggga aacattcggg agaaaataag
     1861 aaaaatgact agcataggag gaatttttta tgacaacaac aatctcaaca aaccaattga
     1921 aggatctaat ccaatatcgt ttaccagaat atcaagcact tgctctagat atccacgaac
     1981 atcctgaagt aagtaactat gagttttatt cctcagcagc gttgattaaa caattagaaa
     2041 aagaaaggtt ttgggtagaa aaagaagtag ctggacaccg aacagccttt gatgcacgtt
     2101 atatttccaa taaacccggt cccacaattg cctttttggc agaattcgat gctttaccag
     2161 gtattggtca tgcatgcggg cataatttgt ttggtactta ctccgtatta gccgccagca
     2221 tagtgaagca attcattgat gaaactggtg gagaaatccg tgtatatggc accccagggg
     2281 aagaaggggg cgagaacgga tcagctaaag gcagctttgt tcgagaaggt ttttttgagg
     2341 atgtcgatgt agcgttatgc gtccatccag cttttcgtta tgggaaaacg acagaatctt
     2401 tagcgaatga tccagtcgac atcaaatttt acggtgtcgc ttcccatgca gcagcagcac
     2461 ctgaaaaagg agtaaatgct ttggaagcat tgatccaagt gtttaatgga atcaacgctc
     2521 tacgcttgca attgccaaaa gatgtcaata tccatgggat cattacagac ggaggagtag
     2581 ctgccaacgt cattccagat tacgctgctg gacgtttcta tttacgtgca ggaaatcggc
     2641 aaacattaga taaagtatat gaaaaagtgg aaaatatcgt acgaggtgct gcgataagta
     2701 cgggcacgac ttttgagttt ggattgttcc aaaatgcagt tgatgatgtc atcgttactc
     2761 cttcttttga tgacttattc ttccagcatg caaaagaagc aggtgttcca gatgaagaaa
     2821 tagaaacgga acaaagaaca agtctcggtt cttctgatgt aggaaatgtc agccaagtca
     2881 taccaacaat ccagcctacg gtagcaatct ctgagacata tattgccgga cacacagaag
     2941 aattcaaagc tgctgctaaa agtgacaaag gccttgcttc tattgcgatt gcagcagaac
     3001 tactcgccaa tacagctctt gatctgttga aagagccaaa gctattagaa agaatcaagc
     3061 aagaacatca agaaatagta gcaagaaaaa aacaagaggg cgctactttc tagtaaaaaa
     3121 taaacttgtg tgtgaaagtt ataaaaataa ttttcagaaa aatgaaaaaa tattttcttt
     3181 ttcattgaca aatgatctta tggaaggtaa acttcgtaca agtcaacttt tcacgcagta
     3241 gcggttaggt gttttgttca gaaagtcggt ggtcgctgtg aaccgatcaa gcctaattga
     3301 cgaattacat ggaaagtcca tacatttgaa taaaagtttg aaagtgaaaa acagatctgt
     3361 ttttaattaa ggtggtaccg cggagtcatt cgtccttagt ttaaaacagg tctttttatt
     3421 tttataaagg aggaatcgcg gatgaattgc ggcggagtaa tccgatatct taactttttt
     3481 agaacataag aacaactaaa aaaggagaga ttatcatggg aaaaaaagga acaccaagtt
     3541 ttttagaagc aacacttgta ttaatggtta ttgttttagg cattgcgatt ggcgttgtcg
     3601 gattaaaggt ctcacctaat atcacgatct tagcagcgat cggaatggtc atgttgtatg
     3661 cagtgataaa aagatacccg acagattggc tgcacgaagg aatcatcaat ggcatcaaac
     3721 caggaatcat cccaatcttt atttttattt tagtcggttc attgattgct gtatggatac
     3781 aagcagggat cattccaact ctaatggtca ttgggttcaa attgatcagt gtccagtggt
     3841 ttgtcccttc tgtatttgtt gtgtgtgcaa tcgtaggtag cgctgtaggg agtgccttta
     3901 ctgttatgtc aacgattggg attgcatttt tcggtattgg gacaacacta ggcttaaatc
     3961 cagcgctggt tgttggagct attgtttcag gtgccgtttt tggagataag atgtctccat
     4021 tatccgaatc aacaaatcta gcagcagcta ttgttgatgc cgatttgttc aaacacatca
     4081 agcatatgat gtggtcaact gtcccggcat ttgttgtttc tttcttttta ttcatgattt
     4141 taggacacac aagtaaagcc gcaagtcttt catctattcg tgaagtgact gatgttctag
     4201 aagcaaactt tactatttca atctggtcac tgattccttt atttttaatg ttactgtgtg
     4261 catggaaaaa ggtgccggca attttaacga ttttgttgaa tatcgctgta gcagtaggaa
     4321 tgatttttat tcaaaaccca caaacttcct taacgaagtt ggctggggtc atcgaatcag
     4381 ggtttgtttc aaatactgga aaccaacaaa tcgattcact gcttagccgc ggaggaatcg
     4441 aaagtatgat gcctactgtg tcattgatta ttctaacttt gtcattaggt ggattgttga
     4501 tcgaatttgg cttgatctca accgtgatgg aacgagtatc aagaaagatg acgaacacac
     4561 caaatttgat ttttacaaca ttgatgacaa gtatcggagt aaatattttt attggtgaac
     4621 aattcctatc tgttattctg cctggtaatg catttaaaga aacttatgaa aaagcaggat
     4681 ttgatccaac tgtattaggt agaacgctag aagatggtgg aacagtaatc aactacttag
     4741 tgccttgggg aattgccgga agtttcgtag caagtacatt tggtattcct acactcactt
     4801 acttgccatt cgtatttttc agtttgcttt ctcctgtgtt ttcgatggtc agtgccttca
     4861 caggttgggg aatctcaaaa aaatttgtga aaccgcaaga agcagtaagc aagacagcgc
     4921 aattagaaca ataaaaacat aaaaagggat tgtgacaaga cttttgtcac aatccctttt
     4981 acgaataaac ggtattcaaa agccagtttc tcggtattaa gccaaattta tacaaaaatg
     5041 tggaaagcca tttcacaaaa tttttcttaa tacgcgaaac taagcgactt tttcacaacc
     5101 tctcgataaa cggtgttcaa tcagctgacc ctcggtatta gctcaaaaaa tggcaaaata
     5161 tgaggaacta tttttccatt tgtctctcta atactcaggt cagaacgctg attttacaac
     5221 ctcttaataa acggtgttca aaagcttgca tctgcggtaa taagcctaac caaacgaaaa
     5281 ataggagaag ctatttttgt ttggctgttc ttattacttg atgcagggca gctttttcac
     5341 aaccccttat tcacggcgtt ccagaagtag cttcttctta tttcttgaag ctgataactt
     5401 ctggaacatc ctcttttttt atttggacgg ataatggtca ttccatttct ttgatcattt
     5461 ttttcatttt gtcgatctct tcttcttgtg cctgaatgat ttcttccgct aattttttta
     5521 cttcaggatc ttttaaatca gctcgtttac ttgccaggat agcgatagaa tgatgaggaa
     5581 tcatggcctt catccaagct gtgtcgttga cagtagtttg gcttcgtacc ataaacaagg
     5641 aaccggcaaa aacaagaaga ctagcggcta agatcataac atttatcttt ttgttttcat
     5701 acattttcca catgaacaac agcatgatga ctgccatgac tgcacccatc atcaacgcca
     5761 tgaatactcg tgtacgactg aagaagatat gatcgagcgc atagacattc aagtacatca
     5821 taccaaacat aactaccgtt gaaacaccga tcatcaacaa aaacttccaa tactttttca
     5881 tttaaaacaa actccttttt ttcattcctt tcttctatca tgtcatgagc atggagaaca
     5941 atcaattatt ttgcacagag ggtttttgaa caccgtttac caagaggtca tgagactgtt
     6001 tattcggaaa aaggagttgc aacaagactt ttgtcacaac tcctttcttc aatagctcca
     6061 tctattccac ataaataaaa taaaattttc tatcgatttc attcattcgt caatacgaac
     6121 gcatcattct ttttttgata actgaaataa atgaacaggt aaagcaggcc agtcaataca
     6181 gttaccccaa tggagttcgt gaacaggata ccaaggaaaa tcaatcctaa gacagctaat
     6241 gcaaaaaagc tgattccttt atcccataat gttccttttc ttagtgccag tacaaaggat
     6301 gccgcactga taatgatcca gatcaataat actgtatacg aaagggaacc tgccagatag
     6361 ccaaataatt tgtcaccgac aaaataagaa agagctacac caagataaag aacactggta
     6421 caaaataaaa cagcacgttg cggtacttga tgcttgttta aaacagctag ttttctagag
     6481 gaaccttttc gatccttcaa acggaagaac aaaattcttg aagatgcata cacacccgaa
     6541 ttgatagatg agaacaaagc aaggacaatc acaaaattga cgatatccgc tgcaaatggg
     6601 atattcattt tttcaaatac catcacaaaa ggactgacat tcgtgcctgc taaagtattc
     6661 caaggaaaaa gaatcaaaag caaaaacaaa ggaatgatgt aaaaggaaat gattcttccc
     6721 atgacacctt tgattgcttt aggaatcgca cgtttaggat catctgcttc gcttaccgtg
     6781 atagcaatca gttcagaacc gccgtaagaa tagataacta caagcaaaga actgatgatt
     6841 cccttcatac catgaggagt aaacccacca tggtcagtca attgctgaag ggtagggaaa
     6901 acgcctgtgc ccaacatttg ctgaccgaca agataagtag caaaaatgat cagtaagata
     6961 atgacactga ttttagcaaa agccagccaa tattctgttt ctgcgaataa ccgtaccgaa
     7021 taaagattga tgatcgtagt taaaacagcc aacataaaaa caaataccca ggctggaatg
     7081 tgcgggaacc acaactgcaa aaaggaagca gctgcaacag cttcagcgat gatattgata
     7141 atccacatag accagtagac ccaatcagta aaatcagcag aatgagcacc tagataaggg
     7201 cgtatcaaac cagataatcc gtgatgcgtg tcgccaccgc ttaaaacgat ttttcccaga
     7261 cctttcatca cgatatataa aatcagccca ccaatcaaaa aagcaatcaa tacagaaggg
     7321 ccggcgatcc caatcgcttc gccacttcct ttaaatagac ctgcaccgat tgctccacct
     7381 aaagctaaca tggtgatatg tctggagcta agttgttttt tcaatttttt atcttccata
     7441 aatgacctct ttcttttaaa ttcacaatca aaatttatat taagaataaa tagcaatatt
     7501 taacaatgac taatgataac aaattttcag acaattacaa ctattcagaa aacttttagg
     7561 cgaattttaa tagaagaatg atttatttca tcatattttc atacaaatgt taaattttca
     7621 tacaaatata ggaaacgttt tactggcttt ttactattta aacaggctat cataggaaaa
     7681 agtgacagag aaagtgaggc ggaaaagatg aacaaaaatg acgtactgct agttgcaaaa
     7741 aataaatcac tttatttttt gccttccatc cgctctttag atgtttggtc cggaaaagat
     7801 ttactacagt cggaattcat taaatcagct agacaacagt tggcaaatta tcagctgatc
     7861 gtttttttag attatggatt tgatatggag atggctcgac gcgtgcgccc atttaccaat
     7921 gcaaaaattg tgttattttt ttggaatcac ttcaaagcag aacacgaggc gatgctgaag
     7981 caggcacaag atgaaccggc aatcgatgag atttatcatt ttgatttttt ggaagcaaaa
     8041 gaacttgggc tgaaacataa tagttcattt tattcgaaac atatgaattt ttcttctagc
     8101 gaaccaaaga cggatctatt ttttggtgca acgaataatg ggagaaaaga acgagcagaa
     8161 agctataagc aggaatttat aaaaagagat gttacgacca actatttcat tcttccacat
     8221 cgagggaatg aacaacctgg atatcttact tatgaagagt acctgaaaaa aacaggggaa
     8281 tcgcgcggga ttctggaact gttgcgggag ggtcagcaag gagtgacttt gcggacattt
     8341 gaatccatgt attttcaaaa gaaactagtg acggataatc aagcgattgc tcattatcat
     8401 ttttatcgtc ctgaaaatat ttttttgctt cgagaacgtg atttagatga attaccggaa
     8461 tttctgcata caccatttaa gccaatccta tcagagctat tggaatttta tgatgcagaa
     8521 aattgggcaa aacgatttat cactaacgat tccactattt atgagcaata cgaatatgac
     8581 ctctcctctg ttgaaaacaa ataaaaaacc tacattttga agagattatg tactgaaaca
     8641 aacaagcaca attcttatgg catcatgaga gttgtgtttt ctttttgatc ttttattggg
     8701 cttaaaaaaa gagaaccagc tgctgattca gctgattctc atcagagagc cttttattct
     8761 gcgccaagga ttccttcttt tgcatggact aattgggtca aaaaggcaca atatggtgta
     8821 gcaatatcat atttttgtcc ttttcgccaa atggcaccat tgatgtagtc gatttcagtc
     8881 aaacgatggt ttttgatcag gtcttggtac atagatgggt aatgcaaccc gatgttgtca
     8941 ggatcataag tagaagcaat atgttggtac acttcttctt gatctagtat gattccttct
     9001 tttgctgcga tagaagcaaa ttcagatacg attgttcgaa ccatgctctc tgccgctttt
     9061 tgcgcgccta gttcagcaat attacaatcc aaaatcgtgc ataaaccatt caatgttcca
     9121 tttacacaag cttttcgcca aatggaataa cgaacgttat ggctatagat cggatttaaa
     9181 tttgcttctt gaaagacttc tacaactttt tttgtaaatg cttcgccttc tggttccaag
     9241 ttctctagtt cgatttctcc atcacctaac aatgtgactt ttccgggacc agctagacca
     9301 gctgtccaca tcgtgatccc aaataagata tttttctttg gcacatattt ttccagtaca
     9361 tcttcatgac ctaatccatt tagtaaacac aaaacataag tattgtcagt aataatggat
     9421 tggatcgaac aaaacatatc atctaattga ttggcttttg ttaatgctac gatcaagtcc
     9481 acgtgttcgt tgctttcgat gatttcttct ggtgaataga ttggcagttt tgcgaccact
     9541 tctttaccgt taaagtcagc aatcaaccca tttgtacgaa tggcttcaat gtgagcaggc
     9601 caacgatcga tcaagaggac ctcatttccg ctttgatgta gcatcaatcc gattcggctg
     9661 cccattgctc cggccccagc tattgcgatt ttcatgtttt ttctctcctt tattcaatga
     9721 gttccctttt agtatataga aaaatggaag aaaatagcat tgttttgttt
//
LOCUS       DACEEZ010000093         9618 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20313_2017
            SAMN12639966-rid9879223.denovo.093, whole genome shotgun sequence.
ACCESSION   DACEEZ010000093 DACEEZ010000000
VERSION     DACEEZ010000093.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639966
            Sequence Read Archive: SRR10023158
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9618)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9618)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741683.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741683.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:44:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,016
            CDSs (total)                      :: 2,962
            Genes (coding)                    :: 2,889
            CDSs (with protein)               :: 2,889
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 36 of 73
            Pseudo Genes (incomplete)         :: 29 of 73
            Pseudo Genes (internal stop)      :: 18 of 73
            Pseudo Genes (multiple problems)  :: 8 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9618
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639966-rid9879223.denovo.093"
                     /isolate="E20313_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            120..1094
                     /locus_tag="IWO89_002402"
     CDS             120..1094
                     /locus_tag="IWO89_002402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HAQ0457118.1"
                     /translation="MRQLHKEQIETAMAALKEKTIDHVYFTACGGSQAVLMAGQYLFD
                     KELTVPCHVYTANEFNYAIPKGLSENSLVITCSHSGTTPETVEATKKAREFGAMTICL
                     SNEEGSPLWEAAEYPVHYDWGKDVDASDLNKGILYGLLFNILLVLDGSKKWETCLNEF
                     DKLNDLVIAEKSKFEITAKEWGKNQKRDKIIYTIGSGINYGEIYSTAACWFMEMQWIN
                     SNAIHSGEFFHGPFEITDYDVPFILVKSIGDTRFLDQRVEDFTKKFSNKLMVLDQADF
                     DLESIDSVAKEYIAAVLSGVVIRQMVEAIAFERGHSLDVRRYMWQMKY"
     gene            1076..1537
                     /locus_tag="IWO89_002403"
     CDS             1076..1537
                     /locus_tag="IWO89_002403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIA"
                     /protein_id="HAQ0457119.1"
                     /translation="MANEILINRRLQKMANQLFHIVITTHSTLCEGYLKATKLILMND
                     ATGISAVPFKENTSPDDFEESMKEIISQHHLQPLIILTDLIGGTPNNVSIKQLNNNRI
                     QIIAGINLPLLLELLMAQSAGKSIEEIDFKELLNNSQKSMVHINSLLKDVS"
     gene            1587..2051
                     /locus_tag="IWO89_002404"
     CDS             1587..2051
                     /locus_tag="IWO89_002404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAQ0457120.1"
                     /translation="MIKLVRVDHRLLHGQVIFSWTKQYDINHIIVADNKVPGDPMSVM
                     ALTIAKPADCKLDIVKISDVKGIMETSPNDNYMILIKGPKEAFELVQNLPEIKEINFG
                     GVSKKSESKQYGKAVFLTPEELEATKKILDQEVKIFVQQVPSSSIENVDFSK"
     gene            2065..2814
                     /locus_tag="IWO89_002405"
     CDS             2065..2814
                     /locus_tag="IWO89_002405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAQ0457121.1"
                     /translation="MLLKAILLGLVGVVAVIDSRLIGRQNIGRPLILSTLAGLVLGDL
                     HTGIVLGASLELISMGFVSIGAAGPPNMQLGSIIATAFAILTNSSTETALTIAIPVAV
                     AGEFMSIIMRMIIAQFGHAADKAIENNKFRKAQIIHIYWSFIFNAFVYFIPIFLTVYF
                     GADVVADLVDKIPEMITDALTVSGNMLSALGFAMLLSTMLSKKLYPYFIFGFFIVAYS
                     GLSLIGVTIFAALIAFVMDQVKYGKREEAHG"
     gene            2814..3644
                     /locus_tag="IWO89_002406"
     CDS             2814..3644
                     /locus_tag="IWO89_002406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HAQ0457122.1"
                     /translation="MENSTEKKRLITKKDLNKMCLRSWTIMCSWNYERQMHMGFMYGM
                     APILNKVYKNDEEKKREAYTRHMAFFNCTPQMTSFIMGLCASMEEQNAQAEEGNFNTE
                     SISMIKTSLMGPFAGIGDSFFQGTLRIITFGIGLSFAKQGSILGPILAVGLFAIPSIL
                     AVFYGGKLGYESGNKYLTKLYQEGLMDRVMQFASIIGLAVVGGMVASMVPIVTPLKFT
                     TGGTELVLQDLLDSIIPGLLPLVCTLGIYKLIKKNVSTNMLLLGCILAGFVLSIIGIL
                     "
     gene            3644..4291
                     /locus_tag="IWO89_002407"
     CDS             3644..4291
                     /locus_tag="IWO89_002407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HAQ0457123.1"
                     /translation="MIKALIFDMDGVLIDSEIMYQKWVFNFLKQENLSYPLYSYQKKI
                     GTTASIFDDIEKYNEGCNSLELKGKFTKYWKSQKIDYLDIFPNILIEQLEWLKKKKIK
                     MAIASSSPMDTINEVVTTCKIESYFDCLISGRDLPESKPNPTIFLKASEQLRIPVEEC
                     LVIEDSYNGIKAGKRANMKVLAIKDKRFSQDVSLADDVIYDIKYLRQEVQVKFGI"
     gene            complement(4389..5165)
                     /locus_tag="IWO89_002408"
     CDS             complement(4389..5165)
                     /locus_tag="IWO89_002408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAQ0457124.1"
                     /translation="MNFQDNRPLYLQVEASIRNDIREKKYLPGEQLPTEEELCKIYGV
                     SKITIRKAFKLLAESGTVERLRGKGTFVTQKKESLKLAGVHGFSDSLSTRGHKIRYTV
                     LHSKIIKADEFLANKLQINVTDRVYNIKRLMWEDGAPMGIDNFFASEEKFPTLFEKGS
                     QYTSLYKLLEDDYDVKATSSIMEINGLSATPELSDLLQCIVGDPLFILEKVGKDKNNQ
                     PFHYSQSTVRCDRISYVIEVTENITLDTVHKNDSDPKVID"
     gene            5309..6268
                     /locus_tag="IWO89_002409"
     CDS             5309..6268
                     /locus_tag="IWO89_002409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HAQ0457125.1"
                     /translation="MTYTHLTTDELVIIESYFNIEKPVALVAQSLNRSRQTIYNVYSF
                     LKQGKKVLDYYRQYKQNKSRCGRRSTVLSDEQSTYVQEKVAQGWSPDVIIGRGEFPID
                     CSVRTLYRMFKKGLFDTQSLPMKGKRKPNNHQEKRGKQAFRRTIHERNSLYPHLTKEF
                     GHLEVDTIVGVKHKSAVITLVECLSKVIITLKPTGRRAEDIENRLNDWFKSMPKHLFK
                     SMTFDCGKEFSNWRSISNSNDIAIFFADPGTPSQRGLNEHSNGLLRRDGLPKTMDFRD
                     TDETFIQSVASKRNHIPRKSLNYRTPLEVFLSCIDKDILSSLI"
     gene            6385..6693
                     /locus_tag="IWO89_002410"
     CDS             6385..6693
                     /locus_tag="IWO89_002410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF960 domain-containing protein"
                     /protein_id="HAQ0457126.1"
                     /translation="MFQNKGQRYVTKGVMDSLPVELQALCWNLIDQNVQKQFHLDYLQ
                     IFEFSTEKGNQKLVHRQEEPEARKEYLISPKLRLKSVSQKIWVIDSGEYQTMLLPEEY
                     "
     gene            6720..7019
                     /locus_tag="IWO89_002411"
     CDS             6720..7019
                     /locus_tag="IWO89_002411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4'-phosphopantetheinyl transferase"
                     /protein_id="HAQ0457127.1"
                     /translation="MLETAIEVLEKCAQLVTTPEEWGYESVTMEKEEIEMGMLPEDVT
                     LPRLVMTHLYIYCAPEDGKDYVVYFITDITSQREFVRGLLVEGRLVWSQIGGTNE"
     gene            7193..7744
                     /locus_tag="IWO89_002412"
     CDS             7193..7744
                     /locus_tag="IWO89_002412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1643 domain-containing protein"
                     /protein_id="HAQ0457128.1"
                     /translation="MIEKELVTTQTEVWRDPHHTHRYVFKRQWAIKGKEAEDKLAVVI
                     TIRPTDTEPFTNDLSMLLIEKNIRQLGFTGFIAVNLFSYTKAKSPRVFPRGNDEQSLE
                     ILTTVFTEKKINTIIFACGSITATSQVAYEQAKTIYDQLSAKQKKMTKVLINAEGKLS
                     HPLSIHVRNEWQLADSQLLFQEK"
     gene            7890..8183
                     /locus_tag="IWO89_002413"
     CDS             7890..8183
                     /locus_tag="IWO89_002413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0457129.1"
                     /translation="MEIDETCLSNSYKSMNDFFEEMELDSELLYQFYLAFRGQQISFP
                     MKMYDRELVRKRIENMIEQEKTVDIRQLTEVYGFSARWIQKVIKQKERRRVHG"
     gene            8176..8472
                     /locus_tag="IWO89_002414"
     CDS             8176..8472
                     /locus_tag="IWO89_002414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0457130.1"
                     /translation="MAKLIVLGGLIYGGYWLYKYMLAHPNIDKGKVIKTVGVIIVGLI
                     ALTKKNNTNSPKIMRTYRCRGCGREISTSFNPSESPQGRDRCHAGYRHSWERIN"
     gene            8543..9547
                     /locus_tag="IWO89_002415"
     CDS             8543..9547
                     /locus_tag="IWO89_002415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4767 domain-containing protein"
                     /protein_id="HAQ0457131.1"
                     /translation="MKKWLLLLIGMGILFTGCTSENTRTKETVETDTTIEAKKSKTQS
                     TEQTTTEMKQSVEASTSESSIIESSSEEVKNTLWDSEKSTQLAIFVPQWGKTLGQEYK
                     SYNPQNNVSLYGTPLPSAVIDGNWKMAINEAPVTVQWSEDGTGHADFNLVAVYSDVET
                     GEYLGQHVYFFGFQNGQPKVYLTQQNQGNDKNYMYFNETQNQQLKQGFIDIVNGQTPQ
                     TPVVEESTQQATTTTTQSTSSSAISSWEAAKETVLNNKALWLYDSPDKNASNTNLIST
                     NEYMKDLMQDENGSYYSINIVTDDNGNTTMAGGKQFRVYTDGRISQRIGMTNNFQMIK
                     "
BASE COUNT         3407 a         1373 c         1797 g         3041 t
ORIGIN      
        1 aaattgttat aaaaaaattt ttttgaaaaa tagtattgca aaataaagca atttcataga
       61 taataggtat tgtaaagtta tatgacttta taatacttat tagagtgagg gataaagaaa
      121 tgagacaact acacaaagaa cagattgaaa cagcaatggc agcgttaaag gaaaagacaa
      181 ttgatcatgt atattttaca gcatgtgggg gttcacaagc cgttttaatg gctggtcaat
      241 atctatttga taaagaatta actgtacctt gtcatgttta tactgctaac gaatttaact
      301 atgctatacc aaaaggatta tctgaaaatt ctttagttat cacttgttcc cattctggca
      361 caacaccaga aactgtagaa gcaacaaaaa aagcaagaga atttggagca atgactattt
      421 gtttatctaa tgaagaagga tctcctctat gggaagcagc tgaatatcct gttcactatg
      481 attggggtaa agatgttgat gctagtgatt taaataaagg aattctttat ggtttattat
      541 ttaatatttt acttgtttta gatggctcta aaaaatggga aacttgcttg aatgaatttg
      601 ataagttaaa tgatcttgtt attgctgaaa agagcaaatt tgaaataact gctaaagaat
      661 ggggtaaaaa ccaaaaacga gataaaatta tttacacaat tggtagcggt atcaattacg
      721 gagaaattta ctccacagca gcttgctggt tcatggaaat gcaatggatt aattctaatg
      781 ctattcattc tggggaattt ttccatggtc cttttgaaat cactgattat gatgtaccat
      841 tcatcttagt gaaaagtatt ggagatacaa gatttttaga tcaacgggta gaagacttca
      901 ctaaaaaatt cagtaataaa ttaatggttt tagaccaagc tgattttgac ttagaatcta
      961 tagatagtgt agcgaaagaa tatattgcag ctgtacttag tggcgttgtt attcgccaaa
     1021 tggtagaagc gattgctttt gaaagaggcc attccttaga tgttagacgc tatatgtggc
     1081 aaatgaaata ttaattaata ggaggttgca aaagatggca aatcaattat ttcacattgt
     1141 gattacaaca cattctaccc tttgtgaggg ttatttaaag gcaacaaaat taattttaat
     1201 gaatgacgcc acaggtatta gtgctgttcc atttaaagaa aatactagtc cagatgattt
     1261 tgaggagagt atgaaagaaa taattagtca gcatcatttg caacctctaa ttattttaac
     1321 ggatttaatt ggaggaacac ctaataatgt ttctattaaa cagttgaaca acaatagaat
     1381 tcaaattatt gctgggatta atttacctct tttactggag ttattaatgg cccagtcagc
     1441 tggtaaatct atagaagaaa tagattttaa ggaattactg aataactctc aaaaaagtat
     1501 ggttcatatt aattcgcttt taaaagatgt cagttaaggt ggcaatttct attctataaa
     1561 ttttaaaaat tgaaggagga aatagaatga ttaaattagt gcgtgtagac cacagattac
     1621 ttcatggaca agtaatattt tcttggacta aacagtatga tattaaccat attattgtgg
     1681 ctgataacaa agttccagga gatccaatgt ctgtgatggc tttgacaatt gctaaacctg
     1741 ctgattgcaa attagacatt gttaaaatta gtgatgttaa agggattatg gaaacatccc
     1801 caaatgataa ttacatgatt ttaattaaag gacctaagga agcttttgaa ttagttcaaa
     1861 atttaccaga aattaaagaa attaattttg gcggtgtgtc taagaaaagc gagtccaaac
     1921 aatatggaaa ggcagtcttt ttaactcctg aagaattaga agccacaaaa aaaattcttg
     1981 atcaagaagt gaaaatattt gtgcaacaag tcccatcttc aagtattgaa aatgtagatt
     2041 ttagtaagta aggggggaaa ataaatgtta ttaaaagcca ttttattagg tttagttggt
     2101 gtggtagctg ttattgactc caggttaatt ggtcgacaaa atattggtag accactaatt
     2161 ttgagtacac ttgccggact tgtgctaggt gacttgcata cagggattgt tcttggggca
     2221 tctttagaat taatttctat gggattcgtc tcaattgggg cggctggacc tcctaatatg
     2281 caacttggta gtattattgc cactgctttt gcaattttaa ctaatagtag tacagaaaca
     2341 gccttaacaa tagctatccc agtggctgta gctggtgaat ttatgagtat tatcatgcgc
     2401 atgataattg ctcaatttgg acatgctgca gataaagcca ttgaaaataa taaatttaga
     2461 aaagctcaaa ttattcatat ttattggtca tttattttta atgcttttgt ttactttatt
     2521 ccaatctttt taacagtata ttttggtgct gacgttgtgg cagatttagt agataaaatt
     2581 ccagaaatga ttacagacgc cttgactgtc tctggaaata tgctttccgc attagggttt
     2641 gcaatgctat taagcacaat gttgagtaaa aaattatatc cttactttat ttttggattc
     2701 tttattgttg cctattcagg tctttcacta attggcgtga caatttttgc agctctgatt
     2761 gcctttgtaa tggatcaagt taaatatgga aaaagagaag aggcgcatgg ttaatggaaa
     2821 atagtacaga aaaaaaacgt ttaatcacaa aaaaagatct taataagatg tgtcttcgtt
     2881 catggacaat catgtgttcg tggaattatg aaagacaaat gcatatggga tttatgtatg
     2941 ggatggcgcc aattttaaat aaagtttata aaaatgatga agagaaaaaa agagaagctt
     3001 atactcgtca tatggcattt tttaactgta cgcctcaaat gacttcattt attatgggac
     3061 tttgtgcttc tatggaagaa caaaatgctc aagcagaaga aggaaacttt aatactgaat
     3121 ctatctccat gattaaaacc agtttaatgg gtccttttgc tggaattggt gattcatttt
     3181 tccaaggtac tttaagaatt attacatttg ggattggatt gtcttttgct aagcaaggta
     3241 gtattttagg accgatttta gctgttggcc tttttgctat accttctatt ttagctgttt
     3301 tttacggcgg taaattagga tatgagtctg gcaataaata ccttactaaa ttgtatcaag
     3361 aaggtcttat ggatcgagta atgcaatttg cttctatcat tggtttggct gtagtaggtg
     3421 gaatggtggc tagcatggtg ccaattgtca cccctttaaa attcactaca gggggaacag
     3481 aacttgtttt acaagactta cttgattcca ttattcctgg attattacca cttgtatgta
     3541 ctttgggaat atataaattg attaagaaaa atgttagtac aaatatgctc ttattaggtt
     3601 gtattttggc agggtttgtt ttaagtataa taggtatctt ataatgatta aggctctaat
     3661 ttttgatatg gacggagttt taattgatag cgagattatg tatcaaaagt gggtatttaa
     3721 ttttttaaaa caagaaaatt taagctatcc tttgtattct tatcaaaaaa agattggaac
     3781 tacagcaagt atttttgatg atatagaaaa gtacaatgag ggatgtaaca gtttagaatt
     3841 gaagggaaaa tttactaaat attggaaatc gcaaaagatt gattacttgg atatttttcc
     3901 taatatacta attgaacagc ttgaatggtt aaaaaagaaa aaaataaaaa tggcaattgc
     3961 ttcttccagt ccaatggata ctataaatga agttgttacc acttgtaaaa tagagtcata
     4021 ttttgattgt ttaattagcg gaagagattt gccagaaagt aaacctaatc ctactatatt
     4081 ccttaaagct tcagaacagc tcagaattcc agttgaagaa tgtctggtga tagaagattc
     4141 ttataatggt ataaaagctg gtaaaagagc aaatatgaag gttcttgcaa ttaaagataa
     4201 acgtttttct caagatgtaa gtctagcaga tgatgttatt tacgatatta aatatctgcg
     4261 acaagaagta caggtgaaat ttggaatata aataagtttt ttgatgtatt tagagaatat
     4321 tagtaaaaat aaaaatcatg cccaaatcta atcaaaattg attgctttgg gtatgatttc
     4381 ttttaatatc aatctattac tttaggatct gaatcatttt tatgcaccgt atctaaagtg
     4441 atattttctg tgacttcaat aacataacta attctatcac aacgaactgt ggattgagaa
     4501 taatgaaatg gttgattatt tttatctttg ccaacctttt ctaaaataaa tagcggatct
     4561 cctactatac attgcaataa gtcggaaagc tcaggtgttg cagataaacc gtttatttcc
     4621 ataatagagc tcgtagcttt gacatcatag tcatcttcta gaagtttata taatgaagta
     4681 tattggctac ctttttcaaa taaagttgga aatttttctt ctgatgcgaa aaaattatca
     4741 attcccatcg gggcaccgtc ttcccacatt aaacgtttaa tattgtaaac tctatcagtt
     4801 acattgattt gtaatttatt agctaaaaac tcatcagcct ttataatttt agaatgtaaa
     4861 acagtatatc taattttatg tcctcgggta gaaagagaat cgctaaatcc atgaactcca
     4921 gctaatttta aactttcttt tttttgagtt acaaaggtcc ctttccccct taatcgttca
     4981 actgttccac tttctgctaa aagtttaaaa gcttttcgaa tggtaatctt acttactcca
     5041 taaattttac ataattcttc ttctgtaggt aattgctctc ctggaagata ttttttttca
     5101 cgaatatcgt tacgtataga agcttctact tgtaaatata atggtctatt atcttgaaaa
     5161 ttcatagtac actccctaaa aagttatact tcattatatc acaatttatt aaaacgtcga
     5221 ttgtaaaatt aagctagaca actaaaaaga tcatttgtgc tacactcaaa atagttccga
     5281 ccaaagaatt aataaggagt gagtacaaat gacttacacc catcttacaa cagacgaact
     5341 agtaatcata gagtcttatt tcaatatcga gaaacctgtc gctcttgtcg cacaaagttt
     5401 aaatcgctct agacaaacca tttataatgt ttattcgttt ctcaaacaag gaaaaaaagt
     5461 gctagattat taccgtcaat acaaacaaaa taaatctcgt tgtggacgac gttcaaccgt
     5521 tttatctgat gaacaatcaa catatgttca agaaaaggta gctcaaggat ggtcacccga
     5581 tgtgattatc ggccgtggcg agtttccaat tgattgttct gttcgtacac tctatcggat
     5641 gtttaaaaaa ggcctttttg acacacagtc gttacctatg aaggggaaac gtaaaccgaa
     5701 taatcatcaa gaaaagcgtg gaaaacaggc ctttcgcaga acgattcatg aacggaacag
     5761 tctctatcca cacctgacta aagaatttgg tcatctcgaa gtcgacacca ttgtgggagt
     5821 taaacacaag agcgctgtta ttacattggt tgagtgttta tccaaggtta ttattacttt
     5881 gaaaccgaca ggcagacgag ccgaagatat cgaaaacagg ttaaatgatt ggttcaaatc
     5941 catgcctaaa cacttgttta aatccatgac atttgattgt ggaaaagaat tttccaactg
     6001 gcgatctata agtaactcga acgatattgc catctttttc gcagatccag gaacaccttc
     6061 gcaaagaggg ctaaatgagc actcaaacgg cttgcttaga cgagatggct tacctaaaac
     6121 aatggacttt agggacacgg atgaaacctt tattcaatcc gttgcctcga aaagaaacca
     6181 tattccaaga aaatcattaa attatcgaac accattggaa gtatttttga gctgcataga
     6241 taaagacata ttgtctagct taatttgaca aatcagatag tataaaatga atcaaattga
     6301 atatcaaata tatattatat ccatgaagcc caatcttctt taatagaaga ttgggctatt
     6361 tttatggcaa aggagggaaa agaaatgttt caaaataaag gacaacgtta tgtcacaaag
     6421 ggagtaatgg acagtttgcc ggtggaatta caagctttat gttggaatct aatcgatcaa
     6481 aacgttcaaa agcaatttca tttagactat cttcaaatct ttgaattttc tacggaaaaa
     6541 ggaaatcaaa agttggttca tcgtcaagaa gaaccagaag caagaaaaga gtatctgatt
     6601 tcacctaagc ttcgtttgaa atcagtgtct caaaaaatat gggtcattga ttcgggtgag
     6661 tatcaaacga tgttattacc agaagaatac tgatatctaa aagaaaggaa cgaaaggact
     6721 tgttagaaac ggctatcgaa gtactggaaa agtgtgcaca gctagtcact actcctgaag
     6781 agtggggcta tgaatccgtt acgatggaaa aagaggaaat tgaaatgggt atgcttcctg
     6841 aagatgttac tctaccacgt ttagtgatga cacatcttta tatttattgc gcaccagaag
     6901 atggaaaaga ctatgtagtt tatttcatta cagatattac cagccagcga gaatttgttc
     6961 gtgggttact ggtagaagga cgattggttt ggagtcagat tggaggaacg aatgaataag
     7021 atcgattatt tgagaatgat tgcgaaatgt ttagaagata tccttacagc gattcatcaa
     7081 attctgaaga aaaaaggaga aagaatatga aatattttca actagtattg acacttataa
     7141 cggcaatggt gattgaattg cttgaacaaa aattagcata ggagaaaaag agatgattga
     7201 aaaagaatta gtgaccacac aaacagaagt ttggcgagat cctcatcata cccatcgcta
     7261 tgttttcaaa cgacaatggg cgatcaaagg aaaagaagcg gaagataaat tagcggtagt
     7321 cattactatt cgtccaacag atacagaacc atttacgaat gatttgagta tgttattaat
     7381 cgaaaagaat atccgacaat taggatttac tggttttatt gctgtgaatc ttttttcgta
     7441 tacaaaagcg aaaagtcctc gggtatttcc tcgtggaaat gatgaacaat ctttggaaat
     7501 attaacaact gttttcacag aaaagaaaat caacaccatt atttttgctt gtggttcgat
     7561 tacagcgacg agtcaagtgg cttatgaaca agcgaagacc atttacgatc aattgagtgc
     7621 gaaacaaaaa aagatgacca aagtattgat aaatgcagaa ggtaagttat cccacccact
     7681 aagtattcat gttcgtaatg aatggcaatt ggcagatagt caattattat ttcaagaaaa
     7741 ataacatttt attttcttat gttacttata agtagtttta catgcttttt gactttaaaa
     7801 atacatgtga tgatacatgt tatatgaatt atcaatgtac ccagtaatat gctattttca
     7861 ttatgtaaaa aataacaggg ggtgtgtaga tggagattga tgaaacttgt ttgagtaact
     7921 cttataaaag tatgaatgac ttttttgagg aaatggaact ggattcagaa ttgctctatc
     7981 aattttatct tgcttttcga gggcaacaga tttcttttcc aatgaaaatg tacgatcgtg
     8041 agttagttag aaaaagaatc gaaaacatga tagaacagga aaaaactgta gatatccgtc
     8101 aattgacaga agtttatggt ttctctgcaa gatggattca aaaagtaatt aaacaaaaag
     8161 aaaggagacg agtacatggc taagctgatt gtattaggtg gtttaattta cggtggatat
     8221 tggttatata aatatatgct tgcgcatccg aatatagata aaggaaaagt aattaagaca
     8281 gttggggtaa tcatagttgg actcatagct ctaactaaga aaaataatac taatagccca
     8341 aaaataatga gaacttatcg ctgtagagga tgtggaaggg aaatttctac tagttttaat
     8401 ccatctgaaa gccctcaagg aagagatcgt tgtcatgctg gctatcgaca ttcttgggaa
     8461 aggattaact aaatgaacac cattgagatt aattgactga taaaataagt acgcgaggga
     8521 gaaattgaaa gggggaaata aaatgaaaaa atggttgtta ttactaattg gaatggggat
     8581 tttattcact ggttgtacat ctgagaatac gaggactaaa gagactgtag aaactgatac
     8641 aacaattgag gctaaaaaaa gtaaaacgca atcaactgaa caaactacaa cagaaatgaa
     8701 acaatctgtg gaagcaagta caagtgaaag ttcaatcatt gaatcttctt ctgaagaggt
     8761 taaaaatact ttgtgggata gtgagaaatc tactcaacta gcaatttttg tcccacaatg
     8821 gggaaagaca ttaggtcaag aatataaaag ctacaatcct cagaacaatg taagtctgta
     8881 tggtacgcct ttaccaagtg cagttattga tggaaattgg aaaatggcaa tcaatgaagc
     8941 accagttacc gtacaatggt cagaagatgg aactggtcat gcagatttca atttagtagc
     9001 agtttattct gatgtagaaa ctggagaata tttagggcag catgtatatt tctttggctt
     9061 tcaaaatgga caacctaaag tttatttaac gcaacaaaat caaggcaatg ataaaaacta
     9121 catgtacttc aatgaaactc agaaccaaca gttaaaacaa ggtttcattg atattgtgaa
     9181 tggacaaact cctcaaactc cagtagtaga agaatctact caacaagcaa caactacaac
     9241 gacacaatct acaagtagct cagcaatttc aagttgggaa gctgctaaag aaactgtact
     9301 aaataataaa gctttatggt tgtatgattc tcctgataaa aatgcatcaa atactaattt
     9361 aattagcaca aatgaatata tgaaagatct aatgcaagat gaaaatggaa gctactattc
     9421 aattaatatt gtaacggatg ataatggaaa tacaacaatg gctggtggaa aacaattccg
     9481 tgtttataca gatggaagaa tttctcaacg tattggaatg actaacaatt ttcaaatgat
     9541 taaataattg aaaatattat ttatcaaaga aaaactgatt ttgagaaaaa ttatttaatt
     9601 ttcttagaat cagttttt
//
LOCUS       DACEEZ010000094         9360 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20313_2017
            SAMN12639966-rid9879223.denovo.094, whole genome shotgun sequence.
ACCESSION   DACEEZ010000094 DACEEZ010000000
VERSION     DACEEZ010000094.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639966
            Sequence Read Archive: SRR10023158
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9360)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9360)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741683.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741683.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:44:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,016
            CDSs (total)                      :: 2,962
            Genes (coding)                    :: 2,889
            CDSs (with protein)               :: 2,889
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 36 of 73
            Pseudo Genes (incomplete)         :: 29 of 73
            Pseudo Genes (internal stop)      :: 18 of 73
            Pseudo Genes (multiple problems)  :: 8 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9360
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639966-rid9879223.denovo.094"
                     /isolate="E20313_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(91..759)
                     /locus_tag="IWO89_002416"
     CDS             complement(91..759)
                     /locus_tag="IWO89_002416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HAQ0457132.1"
                     /translation="MKKLNLFTELCGIILGAGIYGLAVTGINIPSKLADGGVTGIALL
                     LHNLFGFAPSVTSLLFNLPLLLISLFLFGKHAFLRTIVGTFALVFFLHLWESLHIHFA
                     VDSLLINSLMTGILSGIGCGLVFRFGGSTGGTDIIYQAIEKYFHIKIGKSLFLITFGI
                     LVVSLLYLDLTHFLYTLLSCSILSYTLNKVKYLEIGSVLPQLEAKQTTETFEPLDPLE
                     QLEH"
     gene            893..1180
                     /locus_tag="IWO89_002417"
     CDS             893..1180
                     /locus_tag="IWO89_002417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0457133.1"
                     /translation="MRAADLLTILTKSPKKTEYQGVFLAQEQKLVPVTMVQINKANQF
                     IFLFEHGKAPLAMKDIFLFLMKNREKAVYYQSKEELIPLYGVKEIENKVVI"
     gene            1596..3134
                     /locus_tag="IWO89_002418"
     CDS             1596..3134
                     /locus_tag="IWO89_002418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease/substrate-binding
                     protein"
                     /protein_id="HAQ0457134.1"
                     /translation="MNNFIQTLSERKGELLEAAFQHLSISLISLLIAALIAIPLAIWA
                     ANHKKMAEVLLQITGVLQTIPSLALLGLLIPFVGIGTVPALIALVIYALLPIFQNTYI
                     GLAEIDPSIEEAAVAFGMSRMRRLLKVELPIALPVIISGIRTALVLIIGTATLAALIG
                     AGGLGTFILLGIDRNTPVLTLIGAISSALLAIIFSGLIRWLQHKKPRYSIITLAAVVC
                     LFGGVSLYQSALFKNETITIAGKLGAEPEILIQMYKELIEDNTKTEVELKPNFGKTSF
                     LFNALDQGQIDIYPEFTGTVLESLVKVPEAMKSADLTKEKTYDEAEELLNKQFDMTLL
                     KPMAYQNTYALAVKRSFANEHQLKKISDLAAISDQIKAGFTLEFIDRSDGYKGIQEAY
                     GLNFSSVQSMEPSLRYQAINNGDVNIVDAYSTDSQLKEYDLVTLEDDRGLFPAYQGAP
                     LMKKDFAKEHPEIVKALNKLSGKITEDQMIDMNYQVNVEKKKPTEVARIFLKNEGLVK
                     EEDQ"
     gene            3131..4096
                     /locus_tag="IWO89_002419"
     CDS             3131..4096
                     /locus_tag="IWO89_002419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ0457135.1"
                     /translation="MTKLIEFKHVQKKYDGKYVIDDLNLAIKKGEIFVLVGPSGSGKT
                     TTLKMINGLSKPSAGDIYFKGKSLNEYNLQKMRWNMGYVLQQIALFPTMTVKQNIEVI
                     PEMLGWEKQKRADRVDELLQKVGLSPDIYRDRMPRELSGGEQQRIGIIRAIAASPDVI
                     LMDEPFSALDPISRNSLQELVLSLHEELGTTIVFVTHNMEEAIKLGDRIAFMKDGEII
                     QCDTPEQLLMNPKNDYVRHFFDEPKQTKEWRVEDLVVNGYFLNEIPENARQQVCFDTP
                     MKEVYSLLSVYPSIVIVEKQRSIGSLTSKEIFAFLSREEEGNENI"
     gene            4083..5405
                     /locus_tag="IWO89_002420"
     CDS             4083..5405
                     /locus_tag="IWO89_002420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HAQ0457136.1"
                     /translation="MKTYDAIIIGSGVSGLSAAYGLKEAGKTVLVVEEDLWGGTCPNR
                     GCDPKKVLLSAVEARNRVKQLSGKGFNEIPTANWEELQKFKRTFTDPVPESRKKQLAE
                     AEIDHLSGTARFLDDSSIEVNEEVFHADYLVLATGQRPTILPVEGKEYLKTSADFLSL
                     PVLPKEIIFIGGGYIAFELATIANAAGSKVTIVHHNQRPLKEFEASLVEEAVHQMEAS
                     GIQFAFGVETQKIISEGTRYRLVGKETELVADMIFCATGRQPNTESLALEQANIVFDK
                     HGIAVNDYLQTSNPKIFACGDIVSRKTPKLTPVATFEGNYVAKRITDATSEPIKYPII
                     PTIVYASPKLAEVGVTKSHASSSDQVVEMDLTSWFTYHRVNEPVAKAELTFDQQNYLI
                     GAAVISEQADELIDDLTLVINQKLTKKELDSYIMGYPTLASDLSYLLK"
     regulatory      5468..5572
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            5646..6389
                     /locus_tag="IWO89_002421"
     CDS             5646..6389
                     /locus_tag="IWO89_002421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAQ0457137.1"
                     /translation="MKKNLYPAVSMAFILIIWQIVVIVWKIPAFILPGPAAVFSALAA
                     DAQVLLSHSVTTLNEAVLGLVIASVLSFITALAMDYWKFLEMSFYPLLVISQMLPIMV
                     LGPLLTLWFGFGMLPKVILVVLISYFPIVVSFSDTLRKVSKEQIIFLKTMGADTGKIY
                     RIYKIPAGLSGFFSGLKVAATYCVSGAVVGEWLSAQAGLGYYMIRVKNSYQIDKVFAA
                     IICVILLSLLLNGACSLLKKGYYRMLYIF"
     gene            6404..7396
                     /locus_tag="IWO89_002422"
     CDS             6404..7396
                     /locus_tag="IWO89_002422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HAQ0457138.1"
                     /translation="MKKLWLLLPLLLLLSACGTAKETSSKQEIKDLKKVTLVLDYVPN
                     TNHTGIYLAKEKGYYKEAGLNVQIIEPGDNSTSIGLVGADKAQFGVSYQEDVTYAHAD
                     GQNIPVKAIATVIKHNTSGFATLSDSNIHSPKDFEGKTYAGWQSPSEEAVLKAVMEKD
                     GGDFSKLTMVGSNGEGPESLGKSSDIQWYFEGWDMIKAKEAGIELNYIPLKELDERLD
                     YYTPVIITNDQLIKSDPELVQSFMDATKKGYQEAIKDPNDSAKLLQKYAKENDRTFLE
                     ESQAFLSKNYTDDPENWGLMEEKVWRNYTAFMQENGLIKQDVPSQELFTNQFIK"
     gene            7408..8145
                     /locus_tag="IWO89_002423"
     CDS             7408..8145
                     /locus_tag="IWO89_002423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ0457139.1"
                     /translation="MSIQISHLNVVIENLKILEDIHFTIPKDTFTSIVAPSGAGKSTL
                     LKTLTGVQPMTSGTIKVDDQKVTGLHTAFSYMPQEDMLLPWLSVYQNVTLYQKINHLT
                     INEEQVRDYLDIFGLNGYEHFLPEQLSGGMKQRTALLRTIMNPSSYLLLDEPFGALDA
                     MTRGQMQDWMLKLPKKAKRTTLLVTHDIEEAIYLSDRILVLSARPAHVIAEIQVPEKR
                     RSREWLLQQSELKQTIYQLLAGELDVK"
     gene            8135..8857
                     /locus_tag="IWO89_002424"
     CDS             8135..8857
                     /locus_tag="IWO89_002424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HAQ0457140.1"
                     /translation="MLSDAHCHLDGSQSLALLQQEHTILTIINCDSPEEWKENRQLAA
                     SKTQALSYGIHPWKADSYTFEQVEPFLKKARIIGEIGLDNIWTNVPMTTQKKVFERQL
                     AFAAINEKPVVLHTKGCEKEILNYIQAYPNHYLIHWYSSLDYQQDYIDLGCYFSIGLD
                     LKKNPAVWQLAKAVPIDHLLIETDGLHAAEWALGGIFSEKDYVSLLEEQLKTIAEIKE
                     LSVEEVKKNTYQNLERFIRLGK"
     gene            complement(8941..>9360)
                     /locus_tag="IWO89_002425"
     CDS             complement(8941..>9360)
                     /locus_tag="IWO89_002425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase M23"
                     /protein_id="HAQ0457141.1"
                     /translation="YSGQQLTIPTEGSAQAAAEPQAAVQEAPVQAEQPVVQETVQTEK
                     QAAPVAETQPAPAVTETAATPASTSSAKEWIAQKESSGSYTATNGRYIGRYQLDSSYL
                     NGDYSAANQERVAEQYVTSRYGSWEAAKAFWEANGWY"
BASE COUNT         3068 a         1607 c         1972 g         2713 t
ORIGIN      
        1 ttttttataa agaagatttt actgaataat atcattttag tttacataat gatatttctg
       61 taaattaaat aaagtagtga aaaataagaa tcagtgctct aactgttcaa gaggatcaag
      121 tggttcaaat gtttctgtag tctgttttgc ttccaattgg ggcaaaacag atccaatttc
      181 cagatacttc actttattca atgtataaga tagaatagaa cagctaagta atgtatacag
      241 aaaatgtgtg agatccaaat aaagcaagga gacaactaaa ataccaaaag taatcaagaa
      301 aagacttttg ccaatcttaa tatgaaaata tttttcaatg gcttgataaa tgatatctgt
      361 tccgcctgtg cttccgccga atcgaaagac gagaccgcaa ccaatgccag aaagaatacc
      421 tgtcatcaaa ctattgatca gtagactatc tacagcaaaa tgtatatgaa ggctctccca
      481 taaatgtaaa aagaaaacta gcgcaaatgt tccgacgatc gtccggagaa aagcgtgttt
      541 tccaaaaaga aacaatgaga taagcaataa aggtaaatta aaaagcagtg aagtgacaga
      601 aggagcgaat ccaaaaagat tatgcaaaag caaagcaata ccagtaaccc caccatctgc
      661 cagcttagat ggaatattga ttcctgtcac agctaatccg tagattcctg cgcctaaaat
      721 gattccgcac aattctgtaa ataaatttaa ttttttcatt tctagcctct tttcttttga
      781 aaattttcag caacttcttt attatactga cttatactct tacttcaaga ggaaaatgaa
      841 aaaaaattat ttttggctgt ttctttttat ttatcaaata aggagtggaa aaatgcgagc
      901 agcagactta ttgacaattc ttacaaaatc acccaaaaaa acagagtatc aaggagtttt
      961 tttggctcaa gaacaaaaac tggtgcctgt gacaatggtc cagataaata aagcaaatca
     1021 atttatcttt ttatttgaac acggcaaagc accacttgct atgaaagaca tctttctttt
     1081 tttgatgaag aaccgtgaga aagctgttta ttatcagtca aaagaagaac tcattcctct
     1141 ttatggagtg aaagagatag aaaataaagt ggtgatataa gtaaaaataa gaaaacaaga
     1201 cgtttatgat gtcaaaacaa ccatttttta catttttggc aaaaaagtgt catgatacag
     1261 tataatattc gttgaacagg ccaataaagg tgttctcccc cacgagattt ctactttatt
     1321 ggctgtttct ttttattaaa ataaaagttg tgtgtgtaaa aaagatatag ctgattccaa
     1381 gtgacaattt cgtatagatt tttatggtat tcgtagtgaa aaaacgagaa ccaaaaagtt
     1441 gatccgactt tgttgcttgg attctttttt tgtcagcata tttagaggta tttttttctt
     1501 ttttttgttc gtgctgtaag gttaaggcaa gacaagttag ttgggaaaaa ggaaagaaat
     1561 gtttatactt aaagaataac agaaaggaag gtcgcatgaa taattttata caaacattaa
     1621 gtgaaagaaa aggcgagttg ctggaagctg catttcaaca tctgtccatt tctctcattt
     1681 ctttgctgat tgcagcactg attgctattc ctttagcgat ttgggcagcc aatcataaaa
     1741 aaatggcaga agtcttattg cagatcacgg gtgtgctcca aacgattcct tcacttgcct
     1801 tattaggttt acttatccca tttgtaggga tcggaactgt tccagcattg attgcacttg
     1861 ttatttacgc tttgcttccg attttccaaa acacgtatat tggtctagct gaaattgacc
     1921 cgtctattga agaagcagcg gtagcatttg gtatgtccag aatgagacgc ttgctgaaag
     1981 tagagcttcc catcgcatta cctgtcatta tctcaggtat ccgaactgcc ttagttttaa
     2041 tcatcggtac ggctactttg gcagcattga tcggagcagg tggattaggg acatttattt
     2101 tgttaggtat tgaccgaaat acacctgttt tgacattgat tggtgcgatc agttctgcct
     2161 tgcttgcaat tatattcagc ggactgattc gctggttgca gcataagaaa cctcgatatt
     2221 ccatcattac tttagcagca gtggtgtgtt tatttggcgg cgtttcttta tatcagtctg
     2281 ccttgtttaa aaatgaaacc atcacgattg ctggaaaatt aggagcggaa cctgaaatcc
     2341 tgatccaaat gtataaagaa ttgattgaag acaacacaaa aacagaagtt gaattaaaac
     2401 cgaattttgg caagacgagt tttttgttca atgcactgga tcaaggtcaa atcgatatct
     2461 atcctgagtt cacaggtact gtcctagaaa gcttggtgaa ggtacctgaa gcaatgaaaa
     2521 gtgctgattt gacaaaagaa aaaacatatg acgaagcaga agaattattg aataagcaat
     2581 tcgatatgac attattaaaa ccaatggctt accaaaatac gtatgccttg gcagtcaaga
     2641 gatcttttgc gaacgaacat caacttaaaa agatttccga tttggcagct atctctgatc
     2701 agatcaaagc gggattcaca ctggaattta ttgatcgttc agatggatat aaaggaatcc
     2761 aagaagcata tggcttgaat ttttcaagtg tacaaagcat ggagccaagt ttgcgttacc
     2821 aagcaatcaa taatggagat gtcaatattg tggatgccta ttccacagat agccagctca
     2881 aggagtacga tctagtgact ctagaagatg atcgagggtt attcccagct tatcaaggcg
     2941 caccgctgat gaaaaaagat tttgctaaag aacatcctga aatcgttaaa gcactgaata
     3001 aattaagcgg aaaaataacg gaggatcaaa tgattgatat gaattaccaa gtcaatgtcg
     3061 aaaagaaaaa gccgacagaa gtagcacgaa tcttcctgaa aaatgaagga ttagtaaagg
     3121 aggaagatca gtgactaaat taatcgagtt caaacatgtg caaaaaaagt atgatggaaa
     3181 gtatgtcatc gatgacctaa atctggcaat caaaaaagga gaaatctttg ttttggttgg
     3241 tccttccggt agtggaaaaa caactacttt gaaaatgatc aatggattat ctaaaccatc
     3301 agcaggagac atctacttca aaggaaaatc gttaaatgaa tataatctgc agaaaatgcg
     3361 gtggaatatg ggatatgtgc tgcagcaaat cgctttgttc ccgaccatga cagtcaagca
     3421 aaatattgaa gtcatccctg aaatgttagg ttgggaaaag cagaaaagag cagaccgtgt
     3481 agatgaactt cttcaaaaag ttggcttatc tcctgatatt tatcgagatc gtatgcctag
     3541 agagttgtct ggaggagaac agcaacggat cgggatcatc cgagctattg ctgcaagccc
     3601 agatgtgatt ttgatggacg agccatttag cgcattagat ccgatttcaa gaaactcatt
     3661 acaagaattg gttttgtcct tacacgaaga attgggcacg accatcgttt tcgttacgca
     3721 caatatggaa gaagcaatca agctaggtga tcgtattgca tttatgaagg atggagaaat
     3781 tatccaatgt gacacaccag agcagttatt gatgaatcct aaaaacgact acgttcggca
     3841 tttctttgat gaaccgaaac agacgaaaga gtggcgagta gaagatttag tcgtcaatgg
     3901 atatttctta aatgaaatac cagaaaatgc tcgtcaacaa gtttgctttg atacgcctat
     3961 gaaagaagta tattcgcttc tttcggtgta tccaagcatt gtgatagtag aaaagcaaag
     4021 aagtattggg tcgctgacaa gtaaagaaat ctttgctttt ttaagtcgag aggaggaagg
     4081 aaatgaaaac atatgatgca atcatcatcg gttcaggtgt gagcggctta agtgctgctt
     4141 atggattgaa agaagcaggt aaaacagtac tagtggtcga agaagatcta tggggcggga
     4201 cttgtcctaa ccgtggatgt gatcctaaaa aagtcttgtt aagtgcagta gaggcacgta
     4261 atcgggtaaa acaattgagt ggaaaaggtt ttaacgagat cccaactgcc aactgggaag
     4321 aacttcaaaa attcaaacga acatttacgg atccagtccc agaaagccga aaaaaacaat
     4381 tagcagaagc tgaaatcgat catctttcag gaaccgctcg ttttttggat gattcatcta
     4441 ttgaagtcaa cgaagaagtc ttccatgcgg attacttagt tttggcaacc gggcaaagac
     4501 ctacgatctt accagtggag ggaaaggaat acttgaaaac tagtgcagac tttttatcat
     4561 tgcctgtact gcctaaagaa atcattttta tcggtggtgg ttatattgct tttgagctag
     4621 ctacgattgc caatgctgca gggagcaaag taaccattgt ccatcataat caacggccat
     4681 taaaagaatt tgaggcgtct ttggtagagg aagccgtcca tcaaatggaa gcatctggca
     4741 ttcagtttgc ttttggtgtt gaaacccaaa agatcatttc agaaggtact cgctatcgtt
     4801 tagtcggaaa agagacggaa ttagtagcag atatgatttt ttgtgcgact gggagacagc
     4861 cgaatacaga atctttagca cttgagcaag cgaatatcgt ctttgataaa cacggaattg
     4921 cagtcaatga ttatctgcaa acgagcaatc caaaaatttt tgcgtgtgga gatattgttt
     4981 caaggaaaac cccaaaattg acaccagtcg ccacttttga aggaaattat gtagctaagc
     5041 ggataacgga tgcaacaagc gaacctatca aatatccgat tatacctacg atcgtctacg
     5101 ccagtcctaa acttgctgaa gtaggagtta caaaaagtca cgcctcttcg tcagatcaag
     5161 tggtagagat ggatcttacc agttggttca cctatcatcg tgtcaatgaa ccagttgcta
     5221 aggctgaatt gacctttgac cagcaaaatt atttgattgg tgctgcagtg attagcgagc
     5281 aagcagacga attgatcgat gatttgacac tcgtcatcaa tcaaaaattg actaaaaaag
     5341 agttggatag ctatatcatg gggtatccga cattggcaag tgatctctcc tatttattga
     5401 aataatcagc atgataacta aagcgcttgt tgctttttcc ttcttgtgct acactttaaa
     5461 aaatgaatac ctgtttctgg ggatgtcgta gacgacatga gagtaagttg tgaacttaga
     5521 cccattgaac ctgtcagtta atactggtga agggagaaac atagcagacg cttgaagcgg
     5581 gttactatgt cgtgcccatt tcaagcgttt tttgttattc atattagaag aaaggaggta
     5641 acaaaatgaa gaaaaatctt tatccagcag tcagtatggc attcatttta attatttggc
     5701 agattgttgt catagtttgg aaaatacctg cctttatttt acccggacct gctgctgtat
     5761 tttctgcatt agcggcagat gcacaagtat tactttccca ttctgtcacg acattaaatg
     5821 aagcagtgct tggtttggta atagctagcg ttctttcttt tattactgcc ttggcaatgg
     5881 attattggaa gtttttagaa atgtcttttt atccgttact agtgatttca caaatgttgc
     5941 caatcatggt gttagggcca ttacttaccc tatggtttgg tttcggtatg cttccaaaag
     6001 tgatccttgt tgtcttgata agctattttc cgatcgtagt ttctttttcg gatacattga
     6061 ggaaagtatc aaaagagcaa atcatctttt tgaaaacgat gggggcagat acagggaaga
     6121 tttatcgaat atataaaata ccagcgggtt tatccggctt cttttccggt ttaaaagtgg
     6181 ctgctactta ttgtgtcagc ggagcagtcg ttggagaatg gctaagcgct caagcaggct
     6241 tagggtatta catgatacgc gtaaaaaaca gctaccagat cgataaagtt tttgctgcga
     6301 tcatttgtgt cattctattg agtttattgc tgaatggcgc ttgttcatta ttgaaaaaag
     6361 ggtattatcg tatgctttat attttttaag agaaggggat caaatgaaaa aattatggct
     6421 tttattaccg ctactgttgc ttttatccgc ttgtggcact gcaaaagaaa caagtagcaa
     6481 acaagaaata aaggatttga aaaaagtgac acttgttttg gattatgtac cgaatactaa
     6541 tcatactggc atctacctag caaaagaaaa aggctattat aaagaggctg gtttaaatgt
     6601 ccaaatcatc gaaccaggag ataattcgac aagtattggt ctagtaggtg cggacaaagc
     6661 acaatttggt gtaagttatc aagaagatgt cacttatgct cacgcagatg gacagaatat
     6721 accagtcaaa gcaattgcta cagtgatcaa acataatact tctggattcg ccaccctatc
     6781 agacagtaac atccattcac caaaagattt tgaaggaaaa acatatgcag gttggcaaag
     6841 tccaagtgaa gaagctgtct taaaagctgt gatggaaaaa gacggcgggg acttttcaaa
     6901 actgacaatg gtaggaagca atggagaagg gccagaaagc ttaggaaaat cctcagatat
     6961 ccaatggtac tttgaaggtt gggatatgat caaagcaaaa gaagcgggta ttgaactaaa
     7021 ctatatccca ttgaaagaat tagatgaacg gttagattac tatactccgg taattattac
     7081 gaatgatcaa ctgatcaaaa gtgatccaga attggtacag tcttttatgg atgccacgaa
     7141 aaaaggctat caggaagcta ttaaggatcc aaatgacagt gctaaactgt tgcaaaaata
     7201 tgcaaaagaa aatgatcgga cgtttttaga agaatcacaa gcattcttgt caaaaaatta
     7261 tacggatgat cctgagaatt ggggattgat ggaagaaaaa gtttggcgca attatacagc
     7321 ttttatgcag gaaaacggat tgatcaagca agatgtacct tcgcaagaac tattcactaa
     7381 tcagtttata aaataggagt gatagatatg tcaatccaaa tcagccactt gaatgtagtc
     7441 attgaaaacc tgaaaatatt agaagacatc catttcacta tccctaaaga tacctttaca
     7501 tcgattgtcg cgccaagcgg agccggtaag tcaacattgc tgaaaacttt aacaggagtc
     7561 cagcctatga cttctggaac gatcaaagta gacgatcaaa aagtaactgg tctccataca
     7621 gcatttagtt atatgccgca agaagatatg ttacttcctt ggctgagcgt ctatcaaaac
     7681 gttacgttat atcaaaaaat caatcatcta acaatcaatg aagaacaagt aagggattat
     7741 ctggatatct ttgggttaaa cggctatgaa cattttttgc cggaacaatt atctggcggg
     7801 atgaagcagc gaacggcctt gttacgaaca atcatgaatc cttcttctta cttgcttctt
     7861 gatgaaccat ttggagcgtt agatgctatg accagagggc agatgcaaga ctggatgttg
     7921 aagctgccta aaaaagcaaa gcggactact ttacttgtga cgcatgatat cgaagaagca
     7981 atttatttat cagacaggat tcttgtgctt tccgcccgtc cggcacatgt gattgctgag
     8041 attcaagtgc ctgaaaaacg acggtcacgt gaatggctac tgcagcaatc agagttgaag
     8101 caaacgattt atcagttatt ggcaggtgaa ttagatgtta agtgatgctc attgtcattt
     8161 agatgggagt cagagtttag cactccttca gcaggaacac acgattttga cgatcatcaa
     8221 ttgtgattcg ccagaagaat ggaaggaaaa cagacagctt gcagcaagta agactcaagc
     8281 cttaagttac ggtatccatc cttggaaagc agatagctat acgtttgaac aagttgagcc
     8341 ttttttgaaa aaagcaagga tcatcggtga aatagggtta gataatattt ggacgaatgt
     8401 accgatgact acacaaaaaa aagtatttga gcgacagctt gcttttgcag ccataaatga
     8461 gaaacctgtt gtactgcaca caaaaggatg cgaaaaagag atcttaaatt atatccaagc
     8521 ttaccctaat cactatttga tccattggta ttcttctttg gactatcaac aagactatat
     8581 tgatttaggc tgttatttca gcattggtct tgatttgaaa aagaatccag cagtctggca
     8641 actagctaaa gcagtcccaa tcgaccactt acttattgaa acggatggac tacatgcagc
     8701 agagtgggct ttagggggga ttttttcaga aaaagattat gtctcactgc ttgaagaaca
     8761 gttaaagaca atcgctgaga tcaaagagct atcagttgaa gaagttaaaa aaaatactta
     8821 tcaaaacttg gaacgtttca ttagattagg taaataaaaa agagatgtga cacaactagt
     8881 gtcgcacctc tttttggctt tctttgtata aaagtaaact gtttgattaa taaatgaatc
     8941 ttagtaccaa ccgtttgctt cccagaatgc tttcgccgct tcccaagaac cataacgtga
     9001 agtaacatat tgttctgcta ctctttcttg gtttgcagca gagtagtcac catttaagta
     9061 agaagaatct aattggtaac gtccgatgta acgtccgttc gttgctgtgt aagaaccgct
     9121 tgattctttt tgtgcgatcc attcttttgc agagcttgtg cttgctggag tagcagcagt
     9181 ttctgttaca gcaggagcag gttgtgtttc agcaacaggc gctgcttgtt tttccgtttg
     9241 aaccgtttct tgtacaacag gttgttcagc ttggactggt gcttcttgaa cggctgcttg
     9301 aggttcagca gctgcttgtg cagagccttc tgttgggatt gttaattgtt gtcctgagta
//
LOCUS       DACEEZ010000095         9281 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20313_2017
            SAMN12639966-rid9879223.denovo.095, whole genome shotgun sequence.
ACCESSION   DACEEZ010000095 DACEEZ010000000
VERSION     DACEEZ010000095.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639966
            Sequence Read Archive: SRR10023158
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9281)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9281)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741683.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741683.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:44:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,016
            CDSs (total)                      :: 2,962
            Genes (coding)                    :: 2,889
            CDSs (with protein)               :: 2,889
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 36 of 73
            Pseudo Genes (incomplete)         :: 29 of 73
            Pseudo Genes (internal stop)      :: 18 of 73
            Pseudo Genes (multiple problems)  :: 8 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9281
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639966-rid9879223.denovo.095"
                     /isolate="E20313_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            <1..435
                     /locus_tag="IWO89_002426"
     CDS             <1..435
                     /locus_tag="IWO89_002426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase M23"
                     /protein_id="HAQ0457142.1"
                     /translation="YSGQQLTIPTEGSAQAAAEPQAAVQEAPVQAEPVQAEQPVVQET
                     VQTETQAAPVAETQPAPAVTETAATPASTSSAKEWIAQKESSGSYTATNGRYIGRYQL
                     DSSYLNGDYSAANQERVAEQYVTSRYGSWEAAKAFWEANGWY"
     gene            complement(759..842)
                     /locus_tag="IWO89_002427"
     tRNA            complement(759..842)
                     /locus_tag="IWO89_002427"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(806..808),aa:Leu,seq:cag)
     gene            1370..1870
                     /locus_tag="IWO89_002428"
     CDS             1370..1870
                     /locus_tag="IWO89_002428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAQ0457143.1"
                     /translation="MKLIKLWEADLKKAYQLQTSFGPNESGFINNAYGYTFAEFLDYV
                     KKCEKTSLGIDLPESYVPATVFILESDEHEYVGIFNLRHKLNETLRNGAGHIGYGISP
                     NYRKKGYATQGLALVLQEAKKIGIDEALLSVNKKNLGSLKAQQKNGAAIYREDEKEYY
                     TKIKLM"
     gene            complement(2060..2404)
                     /locus_tag="IWO89_002429"
     CDS             complement(2060..2404)
                     /locus_tag="IWO89_002429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sulfur cluster assembly factor"
                     /protein_id="HAQ0457144.1"
                     /translation="MSEANQERSGQEIELIKERILAALEMVIDPELGIDIVNLGLIYD
                     IEFNPENGETVIKMTLTTMGCPLADILTESIHDALKEVPEVTKAEVKLVWYPAWTTDK
                     MSRYARIALGIR"
     gene            complement(2558..3478)
                     /locus_tag="IWO89_002430"
     CDS             complement(2558..3478)
                     /locus_tag="IWO89_002430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HAQ0457145.1"
                     /translation="MTAYHKNERLKKIVVILITGLTAAVALNYFLIPANVFSAGMNGI
                     AQIISSLLYEWFHIKIDTGTFIFLLNIPVFVLGFLKVGKRATILSFVNVVCVSVMTML
                     LPVGQITDNILMNALVGGVLLGLGVGLSLKMGFTTGGMDIISLVLSQTTGKTVGNYML
                     ILNGIIVVIAGFFFNWESALYTIISIYAMTQVIDAIHTSHQKVTAMIVTVKPEEVTEE
                     LAKRMVRGMTLLPSVGGYSKKQGNMIMMVITRYEMYDLDQIVHEIDDDAFINILPTHS
                     VFGRFANENEQKMYRTTGVLPEIKNRSQKK"
     gene            3665..4156
                     /locus_tag="IWO89_002431"
     CDS             3665..4156
                     /locus_tag="IWO89_002431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YajQ family cyclic di-GMP-binding protein"
                     /protein_id="HAQ0457146.1"
                     /translation="MAKDASFDIVSEVNTEEVKNAIQQTTKELSNRFDFKDSTVEIKL
                     ENSNLVVLSDDDFKIEQIKDVLFGKLIKRNVPIKNIHFSESEHALGGKARQTADLVNG
                     IDRENAKKITTAIKNEKLKVKAQIQEDQIRVTGKNRDDLQAVIALLRKLDLPIELQFT
                     NYR"
     gene            complement(4180..6555)
                     /locus_tag="IWO89_002432"
     CDS             complement(4180..6555)
                     /locus_tag="IWO89_002432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase"
                     /protein_id="HAQ0457147.1"
                     /translation="MIKQSKISVRHLVEFILRRGSIDNRKKSNHTALEGARIHRKLQK
                     DAGENYEKEVFLKTTVQLDEYYLIVEGRADGIFKRDGIYYIDEIKTSEPRFEDLEPEQ
                     VELFFHQARVYAYIYSQEHDLQEINLQLTYFQTAEEVITRKVEHQTREQLETFFKKLT
                     EDYKEWLVFQENWRTVRNASLMALKFPHDEYRKGQRELAVAAYKTIRTKQKLFVEAPT
                     GTGKTISTLFPALKAVGEEEGEKIFYLTAKTITRQVAEDAMTALKDTGAEVKSVTLTA
                     KDKICFLDETTCNPDQCPYANGYYNRINEGLWDLLNHENQITREVIETYARKHTLCPF
                     ELSLDVSIWCDVIIGDYNYLFDPTVYLRRFFEDEKNEDYLFLIDEAHNLVNRSREMYS
                     AELSYEKTKRSKEAIPKEFKKLHRRFNKLLKEFDSIREIAKEDHWDYHHQKAPAESLV
                     KAGYQLSEKIKEWLAEFPEHPSQEQLLPYYFDLLHFLKVSEFYDDHYETTVEKTYRDL
                     IVKEFCIDPSLFLEQSLDKGKSSILFSASFSPLSYYQETLGGQKSLAYKLPSPFPEGN
                     QQVFIANYLETTYRKREHSLAKLVETIHAFADGKVGNYFVFFPSYQYLDLAVEAFRQQ
                     YPDSNVLIQETDMNEEERERFLTKFQTNPAETLIGFCVLGGIFSEGIDLRGDRLIGSM
                     IVGVGLPQMNHEQELIKSYYDEKEHLGFAYAYQLPGMNKVLQAGGRVIRGMADQGIVV
                     LADQRFSSFSYRRLFPQHWQTAREVRSVGELEEGIQRFWLSKEAVNQKNEE"
     gene            6881..7507
                     /locus_tag="IWO89_002433"
     CDS             6881..7507
                     /locus_tag="IWO89_002433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HAQ0457148.1"
                     /translation="MHIERLKTGMIEENCYLVYNEEALLIIDPGEDAERIKQEIKKTN
                     QQPVAILLTHTHYDHIGAVEPLRAYYQIPVYVSPLEQKWLGDPILNLSGLGRHDDIAD
                     VIVSPAEYEFEMKTYTLGNMSFRVVPTPGHSIGSVSFIFDDFVVSGDALFRGSIGRTD
                     LYTGNLEQLLHSIRTQLFVLPDEFAVYPGHGDATTIEQEKRTNPFFNR"
     gene            7530..8234
                     /locus_tag="IWO89_002434"
     CDS             7530..8234
                     /locus_tag="IWO89_002434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HAQ0457149.1"
                     /translation="MTETTLYIVRHGKTMFNTIERVQGWCDTPLTKQGQEGIHYLGRG
                     LKNVDFSFAYSSDSGRAIETARIILGEHSKGKEIPYFIEKRIREWCFGSLEGGYDMEM
                     WGVIPRVLNFKTYDEMFTTEVSFEQIANAVYNLDTAGWAETYETLRDRVWTGFEDIAH
                     HCEKNGGGNVLVVSHGLTIAFLLNLIDPKQPVRAGLLNGSVTKVTYANGRFSIHGIND
                     TSYIDNGKTHSRDSFL"
     gene            8426..9259
                     /locus_tag="IWO89_002435"
     CDS             8426..9259
                     /locus_tag="IWO89_002435"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HAQ0457150.1"
                     /translation="MFFANIIKAVILGIIEGITEWLPISSTGHLILADEFIKLDASSQ
                     FMSMFNVVIQLGAILAVVVLYFHKLNPFSPSKSTVEKKDTWTLWFKVIVACIPAIIIG
                     LPLDDWLEANFHKFLPVAIVLIVYGIAFIIVEKRNKNKTPRWTSLNDFTYQAALLVGL
                     FQVLALVPGTSRSGATILGSILIGASRFVATEFSFFLGIPVMFGASGLKIVKYLAEGN
                     SFKMEETVVLLVGTFVSFVVSVLAIKFLLSYLKKNDFTAFGWYRIILGIILIGYWFIA
                     M"
BASE COUNT         2828 a         1791 c         1651 g         3011 t
ORIGIN      
        1 tactcaggac aacaattaac aatcccaaca gaaggctctg cacaagcagc tgctgaacct
       61 caagcagccg ttcaagaagc accagtccaa gctgaacctg ttcaagctga acaacctgtt
      121 gtacaagaaa cggttcaaac ggaaacacaa gcagcgcctg ttgctgaaac acaacctgct
      181 cctgctgtaa cagaaactgc tgctactcca gcaagtacaa gctctgcaaa agaatggatc
      241 gcacaaaaag aatcaagcgg ttcttacaca gcaacgaacg gacgttacat cggccgttac
      301 caattagatt cttcttactt aaatggtgac tactctgctg caaaccaaga aagagtagca
      361 gaacaatatg ttacttcacg ttatggttct tgggaagcgg cgaaagcatt ctgggaagca
      421 aacggttggt actaagattc attatttgaa cgtttaaaca cattcaaaat atagtaagag
      481 gtcatgcgat ttattctttc gcatgacctc tttttgagtt taagtttagt ctttattcct
      541 taactatcgt ttgccagtat ttcttgtatc agaatctata aatctactat ttgaatcaat
      601 ctattaggtt ttaaactgtc ccaccttcac acaaattttt tttgatttat ttgaatctgg
      661 ttccgttgtt taaaattttt cctatattct tttagataat atgttatttt ctttctcatc
      721 gctccaggtg taccttctct ttataaaaaa acaaattatg cggctaagaa gactcgaact
      781 tccacgaggt caccctcact agcgcctgaa gctagcgcgt ctgccaattc cgccatagcc
      841 gcatcacatc ttttagttta ggattacatg tgttttttgt caagaacata tttttgttct
      901 ttttcatttc tgtacgccct cccttatgcc atctacacaa ataattcatc cgagcaactc
      961 catcatctat tgagggttgg tgaaaaaatc tgttttatcc atatgatcga tttcaatgtt
     1021 aaacgaacga ctctgggatt agtaactatt ttattttttt cttcatttct tttttcctct
     1081 ttttctcgat tcagctatct tctttcattt ggatattgat tatttcttct gttgattact
     1141 atccttctgg aattctagac gctgaagtaa ttcacgtttt ctttcttcta gagatcataa
     1201 gtaacgaatg aacgaacttc aaataatttt tttattgtaa ttcatatttg tattgttcga
     1261 tgattcttaa tacgatatga cacaataaat aaaaataggc atggctattt acaacaatag
     1321 tgctatagta gaaaacaaat gaataaggtt acttaaagag gtgaatacga tgaaactgat
     1381 caaactatgg gaagcagatt taaagaaagc ttatcagcta caaactagct ttggaccaaa
     1441 tgagagtgga tttatcaaca atgcttatgg ctatacattt gctgaatttt tagattatgt
     1501 aaaaaaatgc gaaaaaacgt ctttaggaat cgatcttcct gagagttacg ttcccgcaac
     1561 cgtctttatt ttagaaagtg atgaacatga gtatgtagga atttttaatt tgcgccataa
     1621 actgaacgaa acattaagaa acggcgctgg ccatattggt tacggcatct cccctaatta
     1681 tcgtaaaaaa ggatatgcca cacaaggatt agcattagta ctacaagagg caaaaaaaat
     1741 cggcattgat gaggcacttt tgtctgtaaa taaaaaaaat cttggcagtc ttaaagcaca
     1801 acaaaaaaat ggggctgcga tttatcgtga agatgagaaa gaatactata cgaaaatcaa
     1861 actgatgtaa aaaagaagga ttccaaaagg aaagggattt gaatgtcctt tatcaataga
     1921 acacatttat ttaattaact tataatatag cagacaaaat tttataaaca aagaaacctt
     1981 ttatctcttt tcttctagaa aggataaaag gcttttttgt ttttcctatt tgcttctcat
     2041 agctagtcag ccaagatttc tatcgtatcc ccaacgcaat ccgtgcataa cggctcatct
     2101 tatctgtcgt ccatgctgga taccagacta gtttgacttc tgctttcgtt acttctggca
     2161 cttcttttaa tgcgtcatgg atagattctg ttaaaatatc tgctaatgga cagcccatag
     2221 ttgttagagt catcttaatg actgtttctc cattttcagg attgaattct atatcataaa
     2281 tcaagcctaa attgacgatg tcgattccta attcaggatc aatcaccatt tccaaggcag
     2341 caagaatacg ttctttgatt agttctattt cttgtccaga ccgttcttgg tttgcttcgc
     2401 tcatcgtacg acactcctta acacgatttt tatttatcat actctttttt tagctgcttg
     2461 taaatgagaa tttagaagta agattctcgt ttaaatttat aaaaatgttc ttattagatg
     2521 taatagatta aatttattct aaaataaacc tgattcctca ttttttttgt gaacgatttt
     2581 ttatttctgg caatacgcca gttgttcgat acattttttg ttcgttttca tttgcaaacc
     2641 ggccaaatac tgaatgagtt ggtaaaatat tgataaatgc atcgtcgtcg atttcatgaa
     2701 cgatctgatc aagatcatac atttcgtagc gggtgatgac catcatgatc atgttccctt
     2761 gtttttttga atagccaccg acagacggca gtaatgtcat tccacgtacc atccgttttg
     2821 ctagttcttc tgttacctct tctggcttga cggtaacgat cattgctgtt actttttgat
     2881 gactcgtgtg gatggcatca attacttgag tcattgcata aatggaaata atcgtataca
     2941 aggcactttc ccagttaaag aagaagcctg cgatgactac aatgatacca tttagaatca
     3001 gcatgtagtt tccgactgtt tttccagttg tttgtgaaag tacaagagag atgatatcca
     3061 tcccaccagt tgtaaagccc atttttaaag ataatcctac gccaagacct aataaaacac
     3121 cgcctactag ggcattcatc aaaatattat cagtgatctg tccgactgga agcagcatcg
     3181 tcatcaccga tacacataca acattcacaa aactaaggat cgttgctcgt tttcctactt
     3241 tgagaaaccc taatacaaac acagggatat tcaaaagaaa aataaatgtt cctgtgtcta
     3301 tcttgatatg aaaccattca tataaaagcg aggaaatgat ttgagcgatc ccattcatcc
     3361 ctgcagaaaa gacattggct ggaatcaaaa aataatttaa agcgaccgca gcggtcaatc
     3421 cggttatcaa aataaccact attttcttta atctttcatt tttatgatac gcagtcataa
     3481 ttttactcac aagcacctct cccctacttt ggtattccaa atgtaagaat aacagtttat
     3541 ctattaaaat aaaatacttc gaactcaaat gagaagaatt ttagcaaaca ggtaagaatc
     3601 tgccctaatc ctatgataag atagatgtat ggtaaatatt tatatgtata tcaggagggt
     3661 tcctatggca aaagatgcta gcttcgacat tgtatcagaa gtcaatacag aagaagtaaa
     3721 aaatgcgatt cagcaaacaa caaaagaatt gtccaatcgc tttgacttca aggattctac
     3781 agtagaaatc aaattagaga acagcaatct agttgtattg agcgatgatg atttcaaaat
     3841 cgaacagatc aaagatgtat tatttggtaa gctgatcaaa agaaatgtgc caatcaaaaa
     3901 tatccatttt tccgaatcag aacacgctct aggtggcaaa gctcgacaaa ctgccgattt
     3961 ggtcaacgga atcgatcgtg aaaacgcgaa gaaaatcact acagctatca aaaatgaaaa
     4021 actgaaagta aaagcacaaa tccaagaaga tcagattaga gtgacaggta agaaccgtga
     4081 tgatctacaa gctgtgattg ctttattacg taaacttgac ttaccaatcg agttgcaatt
     4141 cactaactat cgttaatcaa aaaacgaggc tgacgtctgt cattcctcgt ttttttgatt
     4201 tactgcttct tttgataacc aaaaacgctg tattccttct tccaattccc caacacttcg
     4261 tacttctctt gctgtttgcc aatgttgtgg gaataaacgt cgataagaaa aactagaaaa
     4321 tcgttgatct gccaatacaa cgattccttg atccgccatg ccacgaatca ctcgaccacc
     4381 agcttgcaag actttgttca ttccaggaag ctgataagcg taagcaaatc caaggtgttc
     4441 cttctcatca taatatgatt tgatcagttc ctgctcatga ttcatctgcg gcaaacctac
     4501 acctacgatc atactgccga tcaatcggtc ccctctcaaa tcgattcctt ctgaaaaaat
     4561 cccgccaagt acgcaaaatc caatcaatgt ttcagcggga tttgtttgaa attttgttaa
     4621 aaatcgttcg cgttcttcct cattcatgtc cgtttcttgg attagcacat tgctgtcggg
     4681 atattgctgt ctgaatgctt ccaccgcaag atctaaatat tgataagaag gaaagaaaac
     4741 gaaataattc ccgacctttc catcagcaaa agcatgaatc gtctctacta attttgccag
     4801 actgtgttcc ctttttcgat aagtcgtctc taggtaattt gcgatgaaaa cttgctggtt
     4861 tccttctggg aaaggactcg gcagtttgta agccagactt ttttgtcccc ccagtgtttc
     4921 ctgataataa gagagtggtg aaaaactagc agaaaaaaga atactgcttt ttcctttatc
     4981 caacgactgc tcaagaaaca agcttggatc aatacaaaac tcttttacta tcagatcgcg
     5041 atacgttttt tcgactgttg tttcgtagtg atcatcataa aattcactga ccttcaaaaa
     5101 atgaagcaaa tcaaaataat aaggaagcaa ttgttcttgt gatgggtgct caggaaattc
     5161 tgccagccat tctttaattt tttcgcttaa ttgataacct gcctttacta aagattcagc
     5221 cggtgctttt tgatgatgat aatcccaatg atcttctttt gctatttctc ggatcgaatc
     5281 aaattctttt aatagtttgt tgaaacggcg atgaagtttt ttgaattcct ttggtatagc
     5341 ttccttactt cgtttcgttt tttcataaga tagttctgcc gaatacatct ctcgggaacg
     5401 attcaccaaa ttatgtgctt cgtcaatcaa aaacagatag tcctcgtttt tttcatcttc
     5461 aaaaaaacgt cttaaataaa cagttggatc aaataaataa ttgtaatccc caatgattac
     5521 gtcacaccag atactgacat ctaatgatag ttcgaaagga caaagtgtat gctttcttgc
     5581 ataagtttcg atgacttcac gagtgatctg attctcatga ttcaaaagat cccataaacc
     5641 ttcattaatc cgattgtaat aaccattggc atatgggcac tgatctggat tacaggtcgt
     5701 ctcatctaaa aagcagatct tgtctttcgc cgtcaaagtg acacttttta cttcagctcc
     5761 tgtatctttt aatgctgtca tagcatcttc agctacttgt ctagttattg ttttagccgt
     5821 tagataaaag atcttttctc cctcttcttc ccctacagct ttcaaagcgg ggaataacgt
     5881 agagattgtt tttcctgttc cggtaggtgc ttctacaaac aatttctgtt tcgtacggat
     5941 cgttttataa gcagctaccg ctagttcacg ctgacctttc ctatactcat catgggggaa
     6001 tttcaaagcc atcaaagagg cattgcgtac agtgcgccag ttttcttgaa aaacaagcca
     6061 ttctttataa tcttcagtca gctttttaaa aaaagtttct aactgctctc ttgtctgatg
     6121 ttcaaccttt ctcgtgatca cttcttctgc cgtctggaag taagtcaatt gcagatttat
     6181 ttcttgtaaa tcatgttctt gactatagat ataggcatac acccttgctt gatgaaagaa
     6241 aagttccact tgttctggtt ctaaatcttc aaatcgtggt tcggaggttt tgatttcatc
     6301 tatataataa ataccgtctc ttttgaaaat accgtctgca cggccttcaa cgatcaggta
     6361 atattcatct aattgaactg ttgtttttaa aaagacttct ttttcatagt tttctcccgc
     6421 atctttttgg agcttgcggt ggattctcgc tccttctaat gcagtatggt tacttttttt
     6481 acgattgtcg atacttccac gtcgcaaaat aaattctact aagtgccgca cagatatttt
     6541 tgactgtttg atcatttcca ccacctcttt ctctctaaga ttacctgaaa aaacctagtt
     6601 tagcaaaaag agctctcttt ccaaacataa aaaaacaccc tgctgattag gcagggatat
     6661 aggatataga atttggattt ttggatgaat ctaatgtaac aaggacatat taaaatgcta
     6721 tgaaaactta ctttccttct ctttagaata tccaaatact tttcatatag tctgataatt
     6781 cagataagta aagagaaata agtacctgtt tttgtttcct agcaatgaat gcgttatact
     6841 aatggatgaa agaagttttg gctatcaagg aggaatgcac atgcatatcg aaagattaaa
     6901 aacaggaatg attgaagaaa actgttattt ggtctacaac gaagaagcac ttttgatcat
     6961 tgaccctgga gaagatgcgg aacgaatcaa acaagaaatc aaaaaaacaa accaacagcc
     7021 ggtagcgatt ttgcttaccc atactcacta cgatcatatc ggtgcagtgg aacctttacg
     7081 cgcgtattat caaatcccag tttatgtcag cccactagag caaaaatggc taggtgatcc
     7141 gattttgaat ctttctggac tcggtagaca tgatgatatt gctgatgtta ttgtctcgcc
     7201 tgcagaatat gaatttgaaa tgaaaactta tactttagga aacatgtctt ttcgcgtcgt
     7261 ccctacccca gggcattcta taggaagcgt cagctttatt tttgatgatt ttgtcgtttc
     7321 tggcgacgcc ctgtttagag gaagtatcgg tagaaccgat ctgtatacag ggaatttaga
     7381 acaattactt catagtattc ggacccagct gtttgtatta cctgatgaat ttgcagtcta
     7441 tccaggacat ggcgatgcaa cgacgattga acaggaaaaa aggacaaatc cttttttcaa
     7501 tcgttaaaac gacttaggag gagaataaaa tgacagaaac tacactttat atcgtacgac
     7561 acggaaaaac gatgttcaat acaattgaac gagtacaagg ctggtgtgat acacccctca
     7621 caaagcaagg acaagaaggc attcattatt taggtcgcgg gttaaaaaat gtcgactttt
     7681 catttgctta ttctagtgac agtggtcgtg caatcgaaac tgcccgtatt attctaggag
     7741 aacattctaa gggaaaagaa attccttatt ttattgaaaa aaggattaga gaatggtgtt
     7801 tcgggtctct tgaaggtgga tatgacatgg aaatgtgggg tgtgatccct cgtgtattga
     7861 attttaaaac atatgatgaa atgtttacca cagaagtttc ttttgaacaa attgctaatg
     7921 ctgtatacaa cttagatacc gctgggtggg cggaaactta tgaaacattg cgagaccgtg
     7981 tatggacagg atttgaagat atcgcccatc actgcgaaaa aaatggtggt ggaaatgttt
     8041 tagtcgtatc tcatggtttg acgatcgcct ttttgcttaa tctgatcgat cctaaacagc
     8101 ctgttcgtgc tgggctttta aatggcagcg taacaaaggt cacttatgca aacggtcgat
     8161 tttctatcca cggcatcaat gatacgtctt atatcgataa cgggaaaaca catagtcgcg
     8221 actcgtttct ataatgggga tattttaatt tttaccagag aatcataaaa ggcaaagtct
     8281 gaacgagggc tttgtcttct tttttctctt cgtgtactta tctgctttat ttctcaagtt
     8341 tttcttaata ttaaaaaaca gattgggttt ctcaaaaaat aacggtatac tataagatgt
     8401 atcttaattg ggaaggaagg aatttatgtt ttttgcaaat attatcaaag cagtgattct
     8461 cggtattatt gaaggaataa cggaatggct gcccatcagt agtactggtc atttgatcct
     8521 agcagatgaa ttcatcaaac tggatgccag ttcacaattt atgtcgatgt tcaatgtcgt
     8581 tatccaatta ggggcgatct tggctgtcgt cgttctttat tttcacaagt taaacccatt
     8641 ttctccaagt aaatcaacgg tagaaaaaaa agatacgtgg acactttggt ttaaagtgat
     8701 cgttgcttgc ataccagcca tcatcattgg tttgcctttg gatgattggt tagaagcaaa
     8761 tttccacaag ttcttaccag ttgccattgt tttgatcgtt tacggtattg cctttattat
     8821 tgtcgaaaaa cgcaacaaaa acaaaacacc tcgttggact tctctgaatg actttacgta
     8881 ccaagcagct cttcttgttg ggttattcca agtcttggca cttgttcccg gaacatcccg
     8941 ttctggcgca acgatcctag gatctatctt gattggtgct tcgcgttttg tcgctacaga
     9001 gttttcattt ttcttaggga ttcctgtcat gtttggcgca agcggcttga agatcgtcaa
     9061 atatttagct gaagggaatt cattcaaaat ggaagaaact gttgttcttc ttgtaggaac
     9121 attcgtatct tttgtggttt ccgtcttagc tatcaaattc ttactaagct atttgaagaa
     9181 aaacgacttt accgcttttg gctggtatcg tattatcctt ggtatcattt tgattggtta
     9241 ttggtttatt gcaatgtaga aaaaaccaga acacttctct t
//
LOCUS       DACEEZ010000096         9216 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20313_2017
            SAMN12639966-rid9879223.denovo.096, whole genome shotgun sequence.
ACCESSION   DACEEZ010000096 DACEEZ010000000
VERSION     DACEEZ010000096.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639966
            Sequence Read Archive: SRR10023158
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9216)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9216)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741683.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741683.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:44:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,016
            CDSs (total)                      :: 2,962
            Genes (coding)                    :: 2,889
            CDSs (with protein)               :: 2,889
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 36 of 73
            Pseudo Genes (incomplete)         :: 29 of 73
            Pseudo Genes (internal stop)      :: 18 of 73
            Pseudo Genes (multiple problems)  :: 8 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9216
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639966-rid9879223.denovo.096"
                     /isolate="E20313_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            23..646
                     /locus_tag="IWO89_002436"
     CDS             23..646
                     /locus_tag="IWO89_002436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0457151.1"
                     /translation="MFVIWEVVKSRVQEMNEHFSVSFAENPSKYFRRLVFLLILSLLL
                     TVAMFLLPEKIREEQVNSLVYSSIPTSKLKPFDYSKADQKIKQSRAISVMFSKPNGKT
                     YQDMLDVFNDPELMEELNRPIFYYPIVYDVHELEQKYNIRTDEVTFIFFDGGKEANRI
                     TAGKGGITDFDKELIPELNRLPLANIKQLEEELDRTGVTSQNSEIAN"
     gene            1096..2145
                     /locus_tag="IWO89_002437"
     CDS             1096..2145
                     /locus_tag="IWO89_002437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HAQ0457152.1"
                     /translation="MKNKRLITVVVIILVYLAGAFIYGKASGEKESTSTNQTKKTTVV
                     GVLQYVSHPALDEIYRGIKDGLKEEGLKEGENLEIKFQNGQADQSKLATMSQQLVQSD
                     PDALIGIATPAAQSLANTTSSIPVVLGAVTDPVGAGLVASLEKPGGNVTGVSDQPPVT
                     SQIKLASDLLPNAKKVGILYASSEDNSKYQVAQAEEAAKKEGLESVKYAVPSTNEIAQ
                     TVQVMSRQVDFIYVPLDNTIANAMQTVVKEANKANIPVIPSVDTMVEQGGLATIGINQ
                     YQLGLQSGKMAAELASGKEKPETTPVYTFDQGDTVINQSQADRLGIIVPQSMKEKAKI
                     ITDDSQQETSKEDDK"
     gene            2142..3023
                     /locus_tag="IWO89_002438"
     CDS             2142..3023
                     /locus_tag="IWO89_002438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAQ0457153.1"
                     /translation="MIVSAISQGMLWAILGLGIFMTYRILDFPDMTTEGSFPLGAAVC
                     VTAITHGVAPIVATLLGVGAGMIAGLVTGLLYTKGKIPVILAGILVMSGLNSVILFVM
                     RTPNLSLLNHPILQDSFHRFGLPDYFDTVLLGFISLGILIAVLLFFFNTDLGQGYIAT
                     GDNETMARSLGIKTDRMKILGLTLSNGVIALSGALIAQNDGYADVNKGIGVIVIGLAS
                     IIIGEVIFQELTLAERLIAIVVGSIIYQLLILVVIKLGFDTTYLKLFSAIILAICLMI
                     PQLKKALNLKTGFEKEA"
     gene            3026..3796
                     /locus_tag="IWO89_002439"
     CDS             3026..3796
                     /locus_tag="IWO89_002439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAQ0457154.1"
                     /translation="MTDILTIKNGRKVVNNGMNEEKVLLDEINLTIREGDFITVLGGN
                     GAGKSTLFNTIAGTLQLSQGSISFKDQLITKDSEEKRARFLSRVFQDPKMGTAPRMTV
                     AENLLLAQKRGQKRKFRLRNLKNQKEDFFRLCQEVGNGLEQHLDTPAGELSGGQRQAL
                     SLLMATITAPELLLLDEHTAALDPKTAKALMRLTNQRINEQKLTCLMITHRMEDALKY
                     GNRLIVLQKGKIVQDISEKEKQHLTMGDLLQFFEEVVD"
     gene            4075..4929
                     /gene="aroE"
                     /locus_tag="IWO89_002440"
     CDS             4075..4929
                     /gene="aroE"
                     /locus_tag="IWO89_002440"
                     /EC_number="1.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HAQ0457155.1"
                     /translation="MISGQTKLAGLFADPASHSLSPLMHNTAFEANNIDAVYLAFQVN
                     PMNLKQAVESIRTFDMLGVNLSMPNKTAVIPYLDEVSQEAQLIGAVNTIVHEKGRLIG
                     YNTDGAGFMQSVQEAGISIKKKKMTLLGAGGAAKAVTVQAALDGAEEIVVYKRNNQTF
                     AQVKETFEQLSYRTSCKIKVRDFADTHQLRKDLKESCLLVNATDIGMGKKINQSPLLD
                     ISMMHENLAVFDLIYAPRETRLLKEAKKVGAKGYNGLGMLIYQGAIAFELWTKEKMPI
                     HVIEKLFD"
     gene            5107..6270
                     /locus_tag="IWO89_002441"
     CDS             5107..6270
                     /locus_tag="IWO89_002441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HAQ0457156.1"
                     /translation="MSRMDRYKDIHKKAKPLKRDTGSIFARRERKRTRMENEEDATRI
                     YEREQHSFEHLNQYEQQIYRDAPRAEEQQKNKKFFNRQKKNKPLKPRRKRSWKKIILS
                     ILLVLILYSGISFFIGKYVAEHDTSLEAATSETFHGQKATNGAHNILILGSDTRGEDA
                     GRADTIMVLQLDGPSHKPKLISFMRDSFVTIPGVGQNKINSAYAYGGADLVRQTLVEN
                     FGIDCQYYAKVDFKSFEKVIDALFMNGVKIDAEKDLNLDGVDIKKGVQKMDGHVLLQY
                     ARFRMDEQGDFGRVRRQQQVMNAIFSQLKNPLNLIRAPYAAGKALGYTSTDVTAWFLT
                     KNLFSIARGAGGIDRISVPVDGSWNYGQSDYAGSILEIDNQMNQSAITDFLNK"
     gene            6387..6797
                     /locus_tag="IWO89_002442"
     CDS             6387..6797
                     /locus_tag="IWO89_002442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HAQ0457157.1"
                     /translation="MDIYLNFKNESREAIAFYEKVFQTEASGITTYGEMPGIEQMNLP
                     EEAKNLITNARLNIYDTLVMFADTPEGIGPELVVGNNVSLVIQTRNEEETKRLFESLA
                     EGGKIEMPLDAVPWAQLYGMVTDRFGINWQINQE"
     gene            6971..9199
                     /gene="topB"
                     /locus_tag="IWO89_002443"
     CDS             6971..9199
                     /gene="topB"
                     /locus_tag="IWO89_002443"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HAQ0457158.1"
                     /translation="MKTVILAEKPSQAKAYAETFQQQKRHEGYYEISDPLFAGEVTIT
                     YGFGHLVDMVAPGAYEERWAKWSLENLPIFPETFRYEVPMDKRAQFSIVKRELQSADT
                     IIIATDGDREGEAIAWSIIRQANAFSKEKNYLRLWINSLEKEAIYEGFKNLQPGKNYF
                     PKYKEAQARQNADWLIGMNGSPLYSLLLQRKGIDGSFSLGRVQTPTLYMIYQLQEEIK
                     HFKKETYFEGEGLITTNKGQFSGKIVPKKAFKTQEELTKEIEKLGAHLGKQNGRILEV
                     TKKEKRLHSPRLFSLSSLQTKMNQLMKASAKATLDAAQGLYESKFLSYPRTDSFYITE
                     NEHQYLVKHLQNYKKFLGIEEVQTTEIKAKKRYVDAKKVQEHHAIIPTKTIPTPARFA
                     KLSKLQQAIYLQVLRTTVAMFAADYIYEETTVMTGVQQLKLKSIGKIPLQQGWQEILR
                     IKSKKKEAQTLPPIVENEEAEVELKSMEKETQPPKPYTEGTLITAMKTAGKTLDNDEA
                     QEILKEVEGIGTEATRANIIENLKQKHYIEVNKNEITVTPKGITLCKAVANEPLLTSA
                     EMTARWEGYLRKIGKQEGTPSVFLENIKKFILHLLAEVPNQIDKVDFSDEISGTRQIK
                     AMEKKNDQLGRCPKCKKGIVMLYPKIATCLNPECDFKLWPTVAKKKLTKTNLRDLLSK
                     GKTSKVVKGFTGKKGKFDAVLVLKEDMTIGFSFPWIENSGTKAVDGNKETSRKNNENE
                     VE"
BASE COUNT         3196 a         1574 c         1996 g         2450 t
ORIGIN      
        1 aggagaaaaa gtagaaaaga aggtgtttgt tatttgggaa gtagttaaat cgagagtaca
       61 agaaatgaat gaacatttct cagtgtcatt cgcagaaaat ccaagtaagt attttcgacg
      121 tcttgttttt ctgctcatct tatcattgct gttaacagtt gcaatgtttt tattgccgga
      181 aaaaatccga gaagagcaag taaatagtct cgtttattcg tccataccaa ccagcaaatt
      241 gaaacctttt gattacagca aagctgatca gaaaatcaaa caaagccggg cgatatcggt
      301 gatgttcagc aaaccgaacg ggaaaaccta tcaggacatg cttgatgttt ttaatgatcc
      361 tgagcttatg gaagaattga atcgaccgat cttttattat ccgatcgttt atgatgtaca
      421 tgaactggaa caaaaatata acatacgaac ggatgaagtg acatttattt tctttgatgg
      481 aggaaaagaa gcaaatcgga taactgcggg taagggagga ataactgatt ttgataagga
      541 gttgatccct gaactgaatc gacttccgtt agccaatatc aaacaattag aagaagaatt
      601 ggatagaacg ggagtaactt cccaaaattc cgagattgct aattgagaca ttggcgcgat
      661 gaaacggtgg gtctatatag cgctattttt aattatcaga acaagtgatg tttgatcttc
      721 aaaataacaa ataaaaaatt aaaaaatata gttgacgaaa ttcaaaaatt cgattagact
      781 taaatcaatt cttaaggaaa gggtgacctg taatgaactt atcaaaacgt atttcagcag
      841 aaaacaaaaa aaattggcgc agctggcgta gataagttgt attaatggtg agactttccc
      901 atagatacaa ctttttagag tgcatttcta aagcttgtat ctatgctaga catgaacatt
      961 gactgccgca tagattagaa tctatgcggt tttttgtgta aagacaatga ccgctttggc
     1021 cattgtcttt ttttgtagac tggatacttg cctgattata ataaataaaa agattgactt
     1081 tgcaggagga aaaaaatgaa aaacaaacga ttaataacag ttgtagtaat tattttagtt
     1141 tatttagcag gtgcatttat ttatggaaaa gcctcaggcg aaaaagaaag tacttcgacg
     1201 aaccagacaa aaaaaacaac agttgttggg gtattgcaat acgtgagcca tccggcatta
     1261 gatgagattt atcgaggaat caaagatggt ctgaaagaag aaggattaaa agaaggagaa
     1321 aatcttgaga tcaaatttca aaatggacaa gccgatcaga gcaagctagc cacaatgagt
     1381 caacagctag tacaatctga tccagatgcc ttgattggaa tcgcaacacc tgccgctcaa
     1441 tcattagcaa acacgacttc atcgatccca gttgtgttag gtgctgtaac ggatcctgtg
     1501 ggggctggat tagtcgcttc tttagagaaa cctggtggta atgtaacagg tgtttctgat
     1561 cagccgcctg ttacttcaca aatcaagctt gcttctgatt tgttgccaaa tgcaaaaaaa
     1621 gtcggaatct tgtatgcttc ttcagaggat aattcaaaat accaagtagc acaagcagaa
     1681 gaagctgcaa aaaaagaagg gttagaatca gtaaaatatg cggtgccttc cactaatgag
     1741 attgcacaaa cggttcaagt gatgagccga caagtcgatt ttatctatgt tccattagat
     1801 aataccattg ccaatgcgat gcaaacagtg gtaaaagaag caaacaaagc aaatatcccc
     1861 gtcattccat ctgtggatac aatggtggaa caaggtggac tagcaacaat cgggatcaat
     1921 caatatcaat tagggcttca aagcggtaag atggcggcag aacttgcgag tggaaaagaa
     1981 aaaccggaaa cgacgcctgt ttatacattt gatcaagggg atacagtgat caatcaatct
     2041 caagctgatc gcttaggaat cattgttccc caatcgatga aggaaaaagc caaaatcatt
     2101 acagatgact cgcagcaaga aacatctaag gaggatgaca aatgatcgta tcagctattt
     2161 cacaaggcat gctttgggca atattaggat tagggatttt catgacttat cggatcttag
     2221 actttcctga tatgacgaca gaagggtcct tcccattagg tgctgctgtc tgtgtgacag
     2281 ctattactca tggggtagcg ccgattgtag ctacattatt aggcgtaggt gctggcatga
     2341 tagcaggact cgttacgggg ttactttata caaaaggaaa gataccggtt attttagcag
     2401 ggattttagt gatgtcagga ttgaactcag ttattttgtt tgtgatgcga acaccaaatc
     2461 tttctttatt gaatcatccg attctacaag acagcttcca tcgctttggg ttacctgatt
     2521 actttgacac agtccttctt ggcttcatct ctttgggcat cttgattgct gtactgctat
     2581 tcttcttcaa tacagatttg ggtcaaggct atattgcgac aggagacaac gaaacgatgg
     2641 ctcggtcgtt agggataaaa accgatcgta tgaaaatttt aggtttaact ttgtcgaacg
     2701 gtgtcattgc attatccggt gcgctgattg cccagaacga cggatatgca gatgtaaata
     2761 aagggattgg cgtaatcgtc atcggtcttg cttccatcat catcggtgaa gtgatcttcc
     2821 aagaattgac gctagcagaa agattgattg ctattgttgt cggcagtatc atttatcagc
     2881 tattgatttt agtagtaatc aaactagggt ttgacacgac ttatctgaag ttgttctccg
     2941 ctatcatctt agctatttgt ctgatgatcc cgcaattgaa gaaagcactg aacttaaaaa
     3001 cgggttttga gaaggaggca tagtaatgac tgatatattg acaatcaaaa atggcagaaa
     3061 agtcgtaaac aatggaatga atgaagagaa agtactattg gatgaaatta accttacaat
     3121 cagagaaggg gacttcatca ctgtccttgg cgggaatggt gctggaaaaa gcacactgtt
     3181 caatacgatc gctggtacgc ttcaactcag tcaaggatcg atctctttta aagatcagct
     3241 gatcacaaaa gattcagaag aaaaaagagc acgattcctt tctcgagttt tccaagaccc
     3301 caagatggga acggctcctc ggatgacagt agcagaaaat ttgctacttg cacaaaaaag
     3361 aggtcaaaaa cggaaattcc gtttacgtaa cttgaaaaat caaaaagaag attttttcag
     3421 actatgtcaa gaagtcggta atggcctaga gcaacacttg gatactccag cgggtgaatt
     3481 atctggtggt caacgccaag cattaagctt gctgatggcg acgatcacag ctcctgagtt
     3541 actattatta gatgaacata ctgcagcttt agatcctaaa acagctaaag cattaatgcg
     3601 attaacaaat cagcggatca atgaacaaaa attgacttgt ctcatgatca cgcatcgaat
     3661 ggaagacgcc ttgaaatacg gaaatcgtct gatcgtactc caaaaaggga aaatcgttca
     3721 agatatttct gaaaaagaaa agcaacattt gacaatgggc gaccttttgc agttctttga
     3781 agaagtagtg gattgatttc gacagaaaaa ttcctttcag tagaaggact tgaaaactca
     3841 ttgacatctt cctttgattc atcctatact taaaacaata aacaattaaa catatttggg
     3901 tgttttgtag gatcacgtag tcaatgattt taacaattaa atagaatatt ctttgctctg
     3961 acaagtaaac gatgagaaaa gaatttgcta ctttgccacc tagacgtttc gtactttttc
     4021 gtatcaaacg ctaagtggct ttttttattg aagtaagaac aaaggaggaa taagatgatt
     4081 tctggacaaa ccaaattagc tgggctcttt gctgatccgg cttctcacag tttatcccca
     4141 ttgatgcaca atactgcttt tgaagcaaac aacatcgatg cggtgtattt agctttccaa
     4201 gtaaatccaa tgaatttgaa gcaagcagtc gaaagtattc gaacttttga tatgttaggt
     4261 gtaaatcttt cgatgccgaa taaaacagcg gttattcctt atctggatga ggtaagccag
     4321 gaagcgcaat tgatcggagc agtgaatacg atcgtgcacg aaaagggtcg tttgattggc
     4381 tataataccg atggtgcggg cttcatgcaa tccgtgcaag aagctgggat ttcgatcaaa
     4441 aagaaaaaaa tgactctact gggagctgga ggtgcagcaa aggcagtcac tgttcaagct
     4501 gcgttagacg gcgctgagga aatcgtagtt tataaacgaa acaaccaaac attcgctcaa
     4561 gtaaaagaaa cattcgaaca actcagctat cgaacttctt gtaagataaa agttcgtgac
     4621 tttgccgata cgcatcaatt aagaaaagac ttgaaagaaa gctgtcttct agtcaatgca
     4681 acagatatcg ggatgggaaa gaaaatcaat cagtcaccgc tcttagatat aagcatgatg
     4741 catgagaatc tagcggtttt tgatttgatt tatgcaccta gagaaacacg tttattaaaa
     4801 gaagcaaaaa aagtaggagc aaaaggatac aatggacttg gcatgttgat ctaccaagga
     4861 gcaatcgcgt ttgaattatg gacaaaggaa aagatgccga tccatgtgat tgaaaagctg
     4921 tttgattaaa aaaggataat agataaagat cttttattct cgcataaacg gtctcaagaa
     4981 aagtaatcct ttatgtatcg aagcatttat ttcgttattt tttattaaat tatcaaaaaa
     5041 tgataggctt ttgtcgattg gttatcgtat cctttattta tcagtaagaa acgaggagga
     5101 accacaatga gtcggatgga tcgctataag gacatccata agaaggcgaa acctctgaag
     5161 cgggatacgg ggagtatttt tgctagaaga gaacgaaaac gaacgcgtat ggaaaatgag
     5221 gaagatgcta cccgtattta tgaaagagag cagcattcat ttgaacattt gaaccaatac
     5281 gagcaacaga tatacagaga tgcaccaaga gcagaagaac aacaaaaaaa taaaaaattt
     5341 ttcaatcggc aaaagaagaa caaaccgtta aaacctcgaa gaaagcgatc gtggaaaaaa
     5401 atcattctat cgattttgct tgttttgatc ctttattcag gtatctcatt ttttattggt
     5461 aaatatgtag ctgaacatga cacaagctta gaggcagcga cttcagaaac ctttcatggg
     5521 caaaaagcga cgaatggtgc gcataatatt ttgatccttg gaagtgatac acgcggagaa
     5581 gatgccgggc gagccgatac gatcatggtc ttacagttgg atggtccttc ccataagccg
     5641 aaactgattt cttttatgcg tgacagcttc gttacgatcc caggggtagg acaaaataag
     5701 atcaattcag cttatgcata cggaggagcc gatctagtgc gtcaaacttt agtagaaaat
     5761 tttggaatcg attgtcaata ctatgcgaaa gtcgatttca aatcgtttga aaaagtgatc
     5821 gatgccttat tcatgaacgg agtgaagatc gatgcagaaa aagatctgaa tctggatggt
     5881 gtagatatta aaaaaggggt ccagaagatg gatggacatg ttcttttgca gtacgcacgt
     5941 ttccgaatgg atgaacaagg agactttggc cgtgtcagac gccaacagca agtaatgaac
     6001 gctattttca gccaattgaa gaatccattg aatctgatcc gtgcacctta tgcagctggt
     6061 aaagctttag gatatacatc cacagatgtc actgcttggt tcttgacgaa aaacctcttt
     6121 tcgattgcac gaggagctgg tggcattgat cgaataagtg tgccagtaga cggttcgtgg
     6181 aattatgggc aaagtgatta tgctggcagt attcttgaaa ttgataatca aatgaatcaa
     6241 tctgctatta cagatttttt gaataaatga tcataaaaaa gatcaagaag aagctgcgca
     6301 aggcttttgt catgaatcaa tcaaacgatt cactcgtaaa aaacgctttc ttatggtaga
     6361 tttaaccaag agaggagctg tttacattgg atatctatct gaacttcaaa aatgagtcgc
     6421 gtgaagccat cgctttttat gaaaaagttt ttcagactga agcatcaggt atcaccacat
     6481 acggtgaaat gcctggaata gaacaaatga acttgccaga agaagcaaaa aatctaatca
     6541 cgaatgcacg gctgaacatt tatgatacat tagttatgtt tgcagatacg ccagaaggaa
     6601 ttggtcctga attagttgtg ggaaataatg tttctttagt catccagaca agaaatgaag
     6661 aagaaacgaa gcgcctattt gaaagcttag ctgagggtgg taagattgag atgccactgg
     6721 atgctgttcc gtgggctcag ttgtatggga tggtaactga tcgatttggc atcaactggc
     6781 agattaacca agagtgacag ctaaagatga atattttctg gaaacatcaa gcagcaaacg
     6841 gatcatcgtt tgtttgcttg atgtttttgt gtctatttcg actatacggt atcttttttt
     6901 tattgccaga tgcgaaaata tgttccataa tagagaagtc cgcaaaaaat taagtaacgg
     6961 aggtcagaag atgaagacgg tgatactagc tgaaaaacct tcacaagcaa aagcctatgc
     7021 cgaaaccttc caacagcaaa agcggcatga agggtattat gaaatcagcg atccattgtt
     7081 tgctggtgaa gtaaccatca cttatggttt tggacatttg gtagatatgg ttgctccagg
     7141 agcttatgaa gagcgttggg caaaatggtc tttagaaaat ttgcctattt tcccagaaac
     7201 cttccgttac gaagttccaa tggacaaacg tgcacaattt tcgatcgtaa aacgagaatt
     7261 gcagtcagcc gataccatca ttattgcaac agatggtgat cgagaaggtg aagccatcgc
     7321 gtggtcgatt attcgacaag caaacgcatt ttctaaggaa aagaactatt tacgtttatg
     7381 gatcaattct cttgaaaaag aagcaatcta tgaaggtttt aagaatctcc aacctggaaa
     7441 gaactatttt ccaaaatata aagaagcgca agcaaggcag aacgctgatt ggctgattgg
     7501 aatgaatgga agtccattat atagcttgct acttcaacga aaagggattg atggcagttt
     7561 ttctcttggt cgtgtacaaa caccgacact ctatatgatt tatcagctgc aagaagaaat
     7621 caaacacttc aaaaaagaaa cgtactttga aggagaaggt ttaattacga caaacaaagg
     7681 gcagttttct ggaaaaatcg taccaaaaaa agcatttaaa acccaagaag agttaacgaa
     7741 ggaaatagaa aaacttggag cgcatttagg taagcaaaac ggacggattc ttgaagttac
     7801 gaaaaaggaa aagaggttgc atagtccacg tttattttcg ctatctagtt tgcagacaaa
     7861 gatgaatcag ctaatgaaag caagtgccaa agctacactt gatgctgctc aaggattgta
     7921 cgaaagcaag tttctaagct atccaagaac ggattccttt tacattacag aaaatgagca
     7981 tcagtatctg gttaaacatc tgcaaaatta taaaaaattt ttaggaatag aagaagtaca
     8041 gacaactgag atcaaagcaa agaaacgata tgtcgatgcg aaaaaagttc aagaacatca
     8101 tgcaattatt ccgacaaaga cgattccgac tcctgcacgc tttgcgaagc tttccaaatt
     8161 acagcaagca atctatttgc aagttttgcg cactactgtg gctatgtttg ctgcagatta
     8221 tatctacgaa gagacgaccg taatgacggg cgtacagcaa ttaaagctaa aaagcatcgg
     8281 aaagatccca ttacagcaag gttggcagga aatcttacgc atcaagtcaa aaaagaaaga
     8341 agctcagact cttcctccta tcgtagaaaa tgaagaagca gaagtagaac tgaaaagtat
     8401 ggagaaagaa acacaacctc caaaacccta taccgaagga acgttgatca cagcaatgaa
     8461 aactgcggga aaaacgctgg ataatgatga agcgcaagaa attctcaaag aagtagaagg
     8521 aatagggaca gaagctacac gagccaatat cattgaaaat cttaagcaga agcattacat
     8581 cgaagtcaat aaaaacgaaa taactgttac gccaaaagga atcactcttt gtaaggcagt
     8641 agccaatgaa ccattactga cgagcgcaga aatgactgcg cgctgggaag ggtacttgag
     8701 aaaaattggt aaacaagagg ggacaccatc tgttttttta gagaatatca aaaaattcat
     8761 cctacattta ttggcagaag ttcctaatca aatcgacaag gtagactttt cagatgagat
     8821 tagcgggaca cgtcagataa aagcgatgga gaagaaaaac gatcaattag gaagatgtcc
     8881 aaaatgtaaa aagggaatag tcatgcttta tcctaaaatc gctacatgct tgaatccgga
     8941 atgcgacttc aaattgtggc caacagtagc taaaaagaag ctgactaaaa cgaatctcag
     9001 agatttgctt tcaaaaggaa agacaagtaa agttgtcaaa ggttttactg gtaaaaaagg
     9061 gaaattcgat gcagttttag tattaaaaga agatatgaca atcggttttt cttttccatg
     9121 gatcgaaaac agtggaacaa aagcagttga tggaaacaaa gaaacatcaa gaaaaaataa
     9181 tgaaaacgaa gtagagtaaa tgaaaacaat gaaata
//
LOCUS       DACEEZ010000097         8904 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20313_2017
            SAMN12639966-rid9879223.denovo.097, whole genome shotgun sequence.
ACCESSION   DACEEZ010000097 DACEEZ010000000
VERSION     DACEEZ010000097.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639966
            Sequence Read Archive: SRR10023158
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8904)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8904)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741683.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741683.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:44:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,016
            CDSs (total)                      :: 2,962
            Genes (coding)                    :: 2,889
            CDSs (with protein)               :: 2,889
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 36 of 73
            Pseudo Genes (incomplete)         :: 29 of 73
            Pseudo Genes (internal stop)      :: 18 of 73
            Pseudo Genes (multiple problems)  :: 8 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8904
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639966-rid9879223.denovo.097"
                     /isolate="E20313_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(305..661)
                     /locus_tag="IWO89_002444"
     CDS             complement(305..661)
                     /locus_tag="IWO89_002444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase"
                     /protein_id="HAQ0457159.1"
                     /translation="MEVKVYGSAHCVTSLKAKQWLEHHQLPYQFVDLEERDLSTEEAE
                     ELCSFEEVQAEQLFATWSEAFHNIAIDLSCVGKKQLIFLCTQHTNLLRRPLIIIDEVL
                     FIGYNEQLMEQLILNK"
     gene            complement(675..1085)
                     /locus_tag="IWO89_002445"
     CDS             complement(675..1085)
                     /locus_tag="IWO89_002445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spx/MgsR family RNA polymerase-binding
                     regulatory protein"
                     /protein_id="HAQ0457160.1"
                     /translation="MIKVYTSPSCTSCRKARAWLQANQLEFEEKNIFAEPLTESEIKK
                     ILAATEGGVQDIISTRSKIYEKLGIDFNELTLKQMIVLFKEYPSLLRRPILIDEKRIL
                     IGFNEDEIRSFVPREIRCVARNLIYLQNNNILNS"
     gene            complement(1432..3618)
                     /locus_tag="IWO89_002446"
     CDS             complement(1432..3618)
                     /locus_tag="IWO89_002446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase"
                     /protein_id="HAQ0457161.1"
                     /translation="MNEKEILRLQKELKRWENKYKKLQQVFRLQEEIIATYEAAYGEA
                     PGLIEEEVQVKESEGNSSFFKMMEKRSYNRFNQEQKAAITYDMKKHLRIIAGAGSGKT
                     QTICAKAVYLMTQKQVDEERILMITFTRNAANELKKRVDNFSQRKTAVHIGTFHSIFF
                     RIYNEICRKFPEVAMQGIQGDFSKDTAQKVNAVLQQLIRKYNLYIFDQYGEKTIASRL
                     DYWQNMNLSVEEMVQLVKEKYDSIDKNARQPISERLYGLLTELQEQKRSQQLLEFNDV
                     LQNLKSALENEEIQHYIGQKYDYLFIDEFQDTNPLQWEIVKRMTKENRIKLIVVGDDD
                     QSIYGFRGSEPAYIKNFEKEYPTKTLFLLTNYRSRAAIVQGANRLISYNKNDRIPKAM
                     IPAQKEAGVMEAYLFSDTKAESQWLIKQIQTFIQKNGKYKESIILYRSASQTQQLVQS
                     LLKTNIPFVLEADSPYEGIFGIKSFQHFYQKIVHWQTALNLQKKNQAYQQLLRQLMAD
                     CYLKKSECDQYFSPKYQNIAVADYILSISPNLKSKSAEIREFEEALRKTDQHQEIYSL
                     VQSYLHLPRIAKEIDQSDQEWIREEVKQHATFRRLQALSQETQATAKELKTRLIAYRQ
                     GKLDALCIQSIHKSKGLSYRNVFLIGCNEGSLPYNGATEKKVIDFNEIKAEPVTTIEE
                     ERRLFYVAMTRARNRLYLCVPQMKNSRKLKVSRFIKETGSRVKKGK"
     gene            3836..4519
                     /locus_tag="IWO89_002447"
     CDS             3836..4519
                     /locus_tag="IWO89_002447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1275 domain-containing protein"
                     /protein_id="HAQ0457162.1"
                     /translation="MQTSFHEGRTIGLLLTFIGGAMDSYTYIQYGAFASAQTGNIILS
                     IIQAFDGEWLSVGKKVLSTLFFFLGILLTKYLIDYFRKKEKHFWRLFVLYYEAVIFFF
                     VSLTPVHEHPALVTILIAFTASIQWVAFDKINGRAYTNLFTTGNLKGVATNLYDYCIS
                     KEKADFDRFFHYLSVVLAFIAGAIVLVFCHHLFGAHSILIVSILFLIMALTQTFHLWQ
                     FYHTNRFTI"
     gene            complement(4600..5319)
                     /locus_tag="IWO89_002448"
     CDS             complement(4600..5319)
                     /locus_tag="IWO89_002448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HAQ0457163.1"
                     /translation="MPIIVIIVAGTIGAGKSTLTEMLAQDLETKPFYENVEDNEVLPL
                     FYSNPEKYTFLLQIFFLNKRFLAIKDAFSHDDNVLDRSIYEDSMLFHLNADLGRVSEV
                     EVKQYEGLLETMLKELEEISPQKKPDLLVYIRVSFETMLARIKKRGREYEQLEQDPEL
                     YSYYKELNRRYEEWYEQFDICPKIVIDGDKYDFVADPACGEQIVQEIKMRAKKMTEEA
                     DAQSIRYTTNNPKAKGIFTNK"
     gene            complement(5631..5837)
                     /locus_tag="IWO89_002449"
     CDS             complement(5631..5837)
                     /locus_tag="IWO89_002449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0457164.1"
                     /translation="MENEQLFEVGETYAFKSDLFESPIKGKIEKIYENSVMIRVMKCA
                     VEDNDTAHNLHWQLVVRKSSTIEE"
     gene            6082..6684
                     /locus_tag="IWO89_002450"
     CDS             6082..6684
                     /locus_tag="IWO89_002450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="accessory regulator AgrB"
                     /protein_id="HAQ0457165.1"
                     /translation="MKDKVVNKLLEALFSGDKSEEDMVYVQVKFALEVLLNNLGKLAV
                     VLVFSLVTGSWAETGITFLSYICIRRYAYGLHSDSEFVCLLWTLLYLWGVPLVMKHLQ
                     LTISFPMMVFLLISCFLLLLRYGSRGTAINPIEPEKRPPLLKKAISMFLIFSLITLFF
                     SASYFSTYLLLGIVLEIATLLPITNYLMNFGGIFHEKNNH"
     gene            6825..8105
                     /locus_tag="IWO89_002451"
     CDS             6825..8105
                     /locus_tag="IWO89_002451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HAQ0457166.1"
                     /translation="MFLWSIALQYLIYTICILLIDHHIRYKYLFAGVILISCILIGWV
                     YSILGIFTALIAIGMLMLTFFFFSTSRQQLVYALPFGLLTSLLGDHLTSIDDFFVLKS
                     SVIEPGDSLQYFHIVLSAFLSALLAFCLKKSLETWFHTVDRRMIGTIGTLILFTYYII
                     IFYTRFSGETPDVLALNTSFFILYLCVGLIILVYYWKISNKKLADQLLEQQVRMQQDY
                     IKDLEKNYQDLREFKHDYQNLLFSLHSYISEGDLEGLRSYYDQTILPTRQMMNLFPAN
                     LSMLDNMKVPEIRSLLSLKLMMAQEKGLIVQLHFPEPIELDTKHTVNLVRMLGIILDN
                     AIEGASAAKKKKIELTILKKNHSVMIRVINTTTNTLPLNQLKQKGFSTKTNPKNEGLG
                     LFILDELTKTNPYLFLETSIENQRFSQTIYIQIK"
     gene            8118..8846
                     /locus_tag="IWO89_002452"
     CDS             8118..8846
                     /locus_tag="IWO89_002452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAQ0457167.1"
                     /translation="MIPIYICEDDPKQLEVLSTVIKNRIMIENLDSYVHISTSDPEEL
                     LNAAKIRTSSFGIYFLDIELEDSEMDGVAIGKLIRELDPLGKIIYVTSHTEMSMKILK
                     SNIEPTDYIVKEDLYDLKENVEHILSKIFEDFQMSQIQKDIFKIEFNDEIKFLPIKDI
                     QYFSTSLGAPHKLEVHLTHAQLQFYEKIKEIEKMHRNFIRCHKSYVINTQNVRSINKK
                     TREVTLANGETIPASIRGLKKLIS"
BASE COUNT         2587 a         1667 c         1463 g         3187 t
ORIGIN      
        1 actcagttat cttatcatgt catttctaac atgtcaacaa ctattttata tgttttttaa
       61 aatactattt tattgctttg ctacttaaaa caacagaaat gaatatatca tgtaaattta
      121 cacatgtcaa tatattttta actctatatc cccacctaga tttgaatttt gaccacataa
      181 attgagcatg tctctatagt agaaaaataa agtcaaaaaa agaacacata acacgcttga
      241 tgctaagtgt tcgactgtta caactgtatg aaatgctcag tgacttgatt aaaacagtta
      301 aagtctattt atttaagata agctgttcca tcaactgctc attgtatcca ataaatagaa
      361 cttcatcgat aataatcaaa ggacgtctga ggagattggt atgttgtgtg cataaaaaaa
      421 tcagttgttt ttttcctaca caggaaaggt caatcgcaat gttgtgaaaa gcctcagacc
      481 aagtcgcaaa tagctgttct gcttgcactt cttcaaaact gcataattct tctgcttctt
      541 ctgtacttag atcgcgttct tctaagtcaa caaattgata aggcaattga tggtgttcta
      601 accattgttt cgccttcaga cttgttacac agtgcgctga tccatagact ttcacttcca
      661 cgaaggctcc ctccctatga atttaagatg ttattgtttt gtaaatagat cagatttctg
      721 gcaacacatc gaatttcacg agggacaaat gaccggatct catcttcatt aaatccgatt
      781 agtattcttt tttcatcaat caaaataggt cgtctcaata atgaaggata ttctttgaat
      841 aagacgatca tttgctttaa agttaattcg ttaaaatcta tgcccagctt ttcgtaaatt
      901 ttcgaccgag tggaaataat atcttgaact cctccttcag tcgctgccaa gatctttttg
      961 atctcacttt ctgttaacgg ttcggcaaaa atattcttct cttcaaattc aagttgattc
     1021 gcttgcaacc atgctcttgc ttttctacag gaagtacagc taggcgaagt atatactttt
     1081 atcattttct cactcatttt taattataat ccaattctac agtaatttct tttaaaatac
     1141 caattattag ctaataatat ataaaaaaaa atcaaaataa ctgattattt acgtttgaat
     1201 aatgagaatt tttctatata ttctctattt tgataatagt attctcattt tcaaaataaa
     1261 taagggaggt gtgacaaaat tcttgtcaca cctccctttt ccgaataaac tgtgttccag
     1321 aaatagcttc ttcgaaaata agctgaaatt tcacaaaaat ttgagaagca attttcgtac
     1381 attccttctt ctttcttgaa actgaccact tctgtcacac cctctcttct atcactttcc
     1441 ttttttcaca cgactcccgg tttctttgat aaatctcgat actttcaatt ttcttgaatt
     1501 tttcatttga ggtacacata gatacagacg atttcttgct ctagtcatcg caacataaaa
     1561 tagtcttcgt tcttcttcta tcgtcgtcac tggttctgct tttatttcat taaagtcgat
     1621 tactttcttt tctgtcgctc cgttataagg caaacttcct tcgttacaac caatcaaaaa
     1681 gacatttcgg taactaagac ctttactttt gtgaatgctc tggatacata gtgcatcaag
     1741 ttttccttgt ctgtaagcaa tcaagcgggt ttttaattct ttagctgtcg cttgggtttc
     1801 ttgcgataag gcttggagtc ttctgaaagt agcatgctgc tttacttctt ctcgtatcca
     1861 ttcttgatca ctctggtcga tttccttagc tatacgtggc agatgaagat agctttgaac
     1921 aagcgaatag atttcttgat gctggtctgt tttacgcaac gcttcctcaa actctcggat
     1981 ttctgcagat ttcgatttta aattggggct tatcgacaaa atataatctg caacggctat
     2041 attttgatat ttaggagaaa agtattgatc gcattcactt tttttcaggt agcagtctgc
     2101 catcagctgc cggagcaatt gctggtaggc ctgatttttc ttttgcagat tcagtgctgt
     2161 ttgccaatga acaatttttt gatagaaatg ttggaaagac ttgataccaa agataccttc
     2221 atatggacta tctgcttcca gtacaaaagg aatattcgtt ttcagcagag attggacaag
     2281 ctgctgcgtt tgtgaagctg atcggtataa tatgattgat tccttatatt tcccattttt
     2341 ttgaataaat gtctgaattt gtttgattag ccactgactt tctgctttag tatcagaaaa
     2401 caaataggct tccataacac ctgcttcttt ttgtgctgga atcatcgcct ttggtatccg
     2461 atcattttta ttatagctga tcaatctatt tgctccttgg acaatagctg cccgagaacg
     2521 ataattagtc aataagaaaa gagttttggt aggatattct ttttcaaagt tcttgatgta
     2581 tgctggctct gatcctctga atccatagat actctgatca tcatctccaa caacaatcaa
     2641 cttgatcctg ttttcctttg tcatccgttt cacgatttcc cattgaagtg gattagtatc
     2701 ttgaaattca tcaatgaata gataatcata tttttggccg atataatgct ggatctcctc
     2761 attctccaaa gcgcttttca gattttggag tacatcattg aactctagca attgctggct
     2821 tcttttttgt tcttggagtt ccgtgagcaa tccatacagt cgttctgata tcggctgtcg
     2881 tgcattctta tcaatggaat catatttttc tttaactaac tgaaccattt cttctactga
     2941 aaggttcata ttctgccaat agtctaaccg agaagcaatc gttttttctc catattgatc
     3001 aaatatataa agattatatt tacgtatcag ctgttgcaaa acagcattca ctttttgtgc
     3061 agtatccttt gaaaagtctc cttgtattcc ttgcattgct acttcaggaa attttcgaca
     3121 aatttcattg tatatcctaa aaaagatact atggaatgtt ccgatatgga cagcggtttt
     3181 tctctggctg aaattatcta ctcgtttttt tagttcattt gcagcgttcc ttgtaaaagt
     3241 gatcattagt atgcgttctt cgtctacttg tttttgcgtc atcagataaa cagcttttgc
     3301 acagattgtt tgcgttttgc cgctcccagc tcctgctatg attcgaagat gtttcttcat
     3361 atcatatgtg atagctgctt tttgttcttg attgaagcga ttataggaac gtttctccat
     3421 cattttaaag aaagagctgt ttccttcact ttcttttact tgtacttctt cctcaataag
     3481 tccgggtgct tcgccataag ccgcttcata ggtagcaatg atttcttctt gtagccgaaa
     3541 gacttgttga agctttttat acttgttctc ccagcgtttc aattcttttt gtaatcggag
     3601 aatttctttt tcattcaact gattgatttc cttcccatct ttcttctttg aatttgttta
     3661 tttcaagttg gttatttaaa gtttcgttgt ctattttttc tttatactaa cagacctttc
     3721 taaaaatgaa aatgggaagc gatatgattt tagttttctg aaaatttttc ctttttttca
     3781 atcgtttatg ctaagataag gaagacaaaa atattcataa aaggtgatga aaaaaatgca
     3841 gacttccttt cacgaggggc gtacaatagg acttttgctg acatttatcg gaggagccat
     3901 ggacagttat acttatatcc aatatggtgc ttttgcctct gctcaaactg gcaatatcat
     3961 tctatcgatc atccaagcat ttgacggaga atggctaagt gttggtaaaa aagtattgtc
     4021 tacccttttc tttttcttag ggattcttct aacaaagtat ctaattgatt attttagaaa
     4081 aaaagaaaaa catttctggc gcttgtttgt tctatattat gaagcagtga tttttttctt
     4141 tgttagtttg acacccgtcc atgagcatcc cgcattagta acgattttga ttgcttttac
     4201 tgcatcgatc cagtgggtag cttttgataa aatcaacggg cgtgcctaca ctaatttatt
     4261 tacaactgga aatttaaaag gggtagcaac gaacttatat gattattgta tttctaaaga
     4321 aaaagcagat tttgatcgat ttttccacta tctaagtgtc gttcttgcct ttatcgcagg
     4381 agctattgtc cttgttttct gtcatcatct gtttggcgcc cattcgattt tgatcgtttc
     4441 cattttattt ttgatcatgg ccctcactca gacatttcat ttatggcaat tttatcatac
     4501 gaatcggttc actatttgat ctcaatgaat caaaaaaact aagcaagatg ctgctcacag
     4561 agaaatatct gcgagtgagc aatttgctta gtttttatat tatttatttg taaagattcc
     4621 ctttgctttc gggttatttg tagtgtagcg gatcgactgg gcatctgcct cttctgtcat
     4681 tttctttgcc cgcatcttga tttcttgtac gatttgttct ccacatgccg gatcggctac
     4741 gaaatcatat ttgtccccat cgatcactat tttgggacat atatcaaact gttcatacca
     4801 ctcttcgtat cgtcgattga gttctttata gtaggaatag agttcaggat cttgttctag
     4861 ctgttcatat tcccggcctc tttttttgat tctcgctaac atagtttcaa aggatacacg
     4921 aatataaacc agtagatctg gtttcttttg tggcgaaatt tcttctaact ccttcagcat
     4981 cgtttctaaa agaccttcat actgctttac ttctacttca gaaacacgcc ctaaatctgc
     5041 attcaaatga aacagcatac tatcttcgta gatggagcgg tctagtacgt tgtcatcatg
     5101 gctgaaagca tctttgattg ccaaaaagcg tttgttcaaa aagaaaatct gtaataaaaa
     5161 agtatacttt tctggattag aataaaatag cggcaatact tcattatctt ccacgttctc
     5221 ataaaaaggt tttgtttcta aatcttgggc aagcatttca gttagtgtgc ttttccctgc
     5281 accgatcgtt cctgcaacaa tgatcacaat tatcggcatc cttcctgtaa taaaatctgt
     5341 tcttcatttt actacttcat ttatagaaat aaaagccttg actttatctg ctccttaaaa
     5401 gctacttctc taaaagtagc caaaagtaac tcctattcat gaaaataaag gctgtgacaa
     5461 aagtcttgtc gcagcccttt tttcaaataa acagtattct cctgccaaca cctcggtcac
     5521 aaggcaatat tcataaaaca tgaaaagctg ttttttaaca ttattccttg taactcagag
     5581 ctaaacggga gactcacaac ctctttatgc tctcaacatt ttatctgttt ttattcttct
     5641 attgtacttg atttgcgtac aactaactgc cagtgcagat tatgagcggt atcattgtct
     5701 tctactgcgc atttcatgac acgtatcatc acactatttt catagatttt ttctattttt
     5761 ccttttattg gtgattcaaa taaatcagat ttaaatgcat atgtttcgcc gacttcaaac
     5821 aattgttcat tttccataag aatccctcca tcacctttta taaggtcagt aattttttaa
     5881 cataaatcac tcgaaaaagc acaaacttgt cataaaaagt acttttcgta tcaaaactgt
     5941 cacttttttt ggtaaggttc ttggaaaacg aagatttttt taggagatca tgacggtttt
     6001 gatgtcaaaa ccacttttta tgaaacaaaa gacgtttttt atttttttta gcataaaatt
     6061 tgtggtggag gtacgtatga gatgaaagat aaagttgtga acaaattatt agaagcctta
     6121 ttcagcggag acaaatcgga agaagatatg gtgtatgtcc aagtaaaatt tgccttagaa
     6181 gttttattga ataatcttgg aaaactagcg gtcgttctag tcttttctct ggtgacaggc
     6241 tcatgggcgg aaacaggaat cacctttctt tcttatatct gcatccgcag atatgcttat
     6301 ggattgcatt ccgactctga attcgtttgt ctgctgtgga cactacttta tttatggggc
     6361 gttcccttgg tgatgaaaca tctgcaattg actatctcct tcccaatgat ggtctttttg
     6421 ttaatcagct gctttttgtt actgcttcgc tatggatcaa gaggtacagc aatcaatccc
     6481 attgaacccg agaaacgtcc cccattatta aagaaggcga tcagcatgtt tctaattttt
     6541 tctctcatta ctttattttt ctcagcttct tacttttcta cctatttgct acttgggatt
     6601 gttttagaaa tagctacgtt gcttcctatt acaaactact tgatgaattt tggaggaatc
     6661 tttcatgaaa aaaacaatca ttaaacaatt aactaactta tctgactttt ttgtatctgg
     6721 ctgctgcttt ggtatgagtt ctgaattcaa aatccctgaa aaattaaaaa aataatacgc
     6781 taattgtgct ataatttggt aaaaatcaat gggagctgta ctttatgttt ttatggtcca
     6841 tagcgttgca atatttgatt tacacaattt gtatcttatt gattgaccat cacatccgat
     6901 acaaatatct gtttgcggga gtcattctga tttcttgtat tttgattgga tgggtctatt
     6961 cgattttagg gatttttact gctctaattg ctataggaat gctgatgctt acttttttct
     7021 tcttttcgac ttcgcgtcaa caattggttt acgcgcttcc ttttgggtta ttgacgtcgc
     7081 ttttaggaga tcacttaaca agcatcgacg atttttttgt tttgaaatct tcggttattg
     7141 aaccaggaga ttcattgcag tattttcaca ttgtcttatc cgctttttta tctgctttat
     7201 tagctttttg tttaaaaaaa tcgttagaga cttggtttca cactgtggac cgccgtatga
     7261 tcggtacgat cgggacattg atattattta catactatat cattattttt tatacccgtt
     7321 tttctggtga aacacctgat gtcttagctt tgaatacgag ttttttcatt ctatatctgt
     7381 gtgttggcct gatcatcttg gtttattatt ggaaaatctc taataagaaa ttagctgatc
     7441 agttattaga gcaacaagtc aggatgcaac aggattatat caaagatcta gaaaaaaact
     7501 atcaagactt gagagaattc aaacatgatt accaaaatct gcttttttct cttcacagct
     7561 atatttcaga aggcgactta gaaggattac ggtcttatta tgatcaaacg atattaccta
     7621 cacgccagat gatgaacctt tttccggcca atctaagtat gttggataat atgaaagttc
     7681 ctgagatccg tagtttactg tcattaaaac tcatgatggc acaggaaaaa gggttgatcg
     7741 tccagcttca ttttcctgag ccaatcgaac tggatacgaa acatacggta aaccttgtac
     7801 gtatgcttgg tattatcttg gacaatgcga ttgaaggagc tagtgctgca aagaagaaaa
     7861 aaatcgaatt gaccatactc aagaagaatc attccgtgat gattcgtgtg atcaatacaa
     7921 caacaaatac attaccgtta aaccagttga aacaaaaagg gttctcaaca aaaacaaatc
     7981 caaaaaacga aggccttggc ttgtttatct tggatgaatt gacaaagacc aatccttatc
     8041 ttttccttga aacatcaatt gaaaaccagc gtttttctca aacgatttat atacaaataa
     8101 aataaaggag taatgatatg attcccattt atatttgtga agacgatcca aaacaattag
     8161 aagttttatc aacggtgatc aaaaatagaa tcatgattga aaacttagac agttatgtcc
     8221 atatttctac ctctgatccg gaagaacttc tcaatgcagc caagatcaga acttcttctt
     8281 ttggtattta ctttttagat atcgaactag aagacagtga aatggatggt gtagcgatcg
     8341 gtaaattgat ccgtgaatta gatcctttag gaaaaataat ctatgtgact tcccatacgg
     8401 aaatgtcgat gaaaatctta aaaagcaaca tcgaaccgac tgattatatc gtcaaagaag
     8461 atctttatga tttaaaagaa aatgtcgagc atatcctgag caaaattttc gaagattttc
     8521 aaatgtccca aatccaaaaa gatatcttca aaatagagtt taatgatgag atcaaatttt
     8581 taccgatcaa agacatccaa tatttttcta cttcgctagg agcgccacat aaactggaag
     8641 tccatttgac tcatgcccaa ttacaattct atgagaagat caaagaaatc gagaaaatgc
     8701 atcggaattt tatccgttgt cataaatcct atgttatcaa tacacaaaat gtgcggtcga
     8761 ttaataaaaa aaccagagaa gtcacgttgg caaacggcga gaccatccca gcctctatcc
     8821 gtggattaaa gaaattgatt tcataaaaag aaccgaatag tttggaccga ctaaatgacc
     8881 agccgatcca aacccttcgg ttct
//
LOCUS       DACEEZ010000098         8576 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20313_2017
            SAMN12639966-rid9879223.denovo.098, whole genome shotgun sequence.
ACCESSION   DACEEZ010000098 DACEEZ010000000
VERSION     DACEEZ010000098.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639966
            Sequence Read Archive: SRR10023158
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8576)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8576)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741683.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741683.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:44:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,016
            CDSs (total)                      :: 2,962
            Genes (coding)                    :: 2,889
            CDSs (with protein)               :: 2,889
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 36 of 73
            Pseudo Genes (incomplete)         :: 29 of 73
            Pseudo Genes (internal stop)      :: 18 of 73
            Pseudo Genes (multiple problems)  :: 8 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8576
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639966-rid9879223.denovo.098"
                     /isolate="E20313_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(<1..180)
                     /locus_tag="IWO89_002453"
     CDS             complement(<1..180)
                     /locus_tag="IWO89_002453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoate
                     polyprenyltransferase"
                     /protein_id="HAQ0457168.1"
                     /translation="MTLKIFLEVVEIRTKVASVFPFAVGVLFSIAFFHEIHWLNTFLF
                     FIGMLVFDLATTAINN"
     gene            complement(193..1296)
                     /locus_tag="IWO89_002454"
     CDS             complement(193..1296)
                     /locus_tag="IWO89_002454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase"
                     /protein_id="HAQ0457169.1"
                     /translation="MRKKLLFSLITLGISFMVLTGCSNSANKTTTESSAATKILKDPY
                     SDEQFLLGTYVRIRVYDEGKESALKPAFDRVKELGDKITINQKGSEIDEVNEQAGIKP
                     VKVSDDVYTLVKRAYEYSQDSQGGFDMAIGAITQLWRIGFDDARKPSQEEIDQALKLV
                     DYHKIELNDKEKTVYLKEKGMIIDLGAIAKGYITDEVVKVLKKQGVTTAIVDLGGNVY
                     VLGHSPRGENQDWTVGIQDPNMARGSVLGSIKERNKTLVTSGIYERYLEVDGKKYHHL
                     FDPKTGYPFDNDIASVTIITDKSIDGDGLSTAVFSMGVKKGLEYVESELNNGTEAIFV
                     TKDDKVYVTDPIKDTFKLSEDSHYTMGDRSELK"
     gene            complement(1386..2321)
                     /locus_tag="IWO89_002455"
     CDS             complement(1386..2321)
                     /locus_tag="IWO89_002455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-binding protein"
                     /protein_id="HAQ0457170.1"
                     /translation="MKKKRFVTGFAVLAFSALVLGACGADDNGSSNSSSESSTAQSST
                     AKSTTESSAKVVAGGDLQDGTYKLEEKNYSNGYRAVFEMVVKDGKITESKYDNVNENG
                     ESKTKDAEYNKNMEAKSGTNPEKYIPELNDQFLKAQSASGVEVVTGATHSSESFQNYA
                     QQLIQAAQAGNTDTIEIDNGADLKDGTYKLEEKNYSNGYRVQFEMTVAGGKVTESNFD
                     YIDKDGKSKQDDTEYNENMKAKSGTEPKTYIPTLNDEFVKAMGEEDGSPANVEVVTGA
                     THSSHSFIMYAQQLVNAAEKGDTQTIEVDNIVTEK"
     gene            2623..4557
                     /locus_tag="IWO89_002456"
     CDS             2623..4557
                     /locus_tag="IWO89_002456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016186137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HAQ0457171.1"
                     /translation="MAKQNIVVVGAGYAGVSATKYLAKKLKKEDVAITLIDRHSYHTM
                     MTELHEVAGGRVEPEAIQYDLQHLFARQKNVQLVTDTVIGIDKERKIVKTKLGSYPFD
                     QLILGMGGEPNDFGTPGVKAHGFTLWSFEDSVRIREHIEKTVAKAALEPDDELRKAML
                     TFVVCGSGFTGIEMIGELIDWKNRLAKDYKLDPNEITLMVVEAMPTILNMLDRNDAAK
                     AERYLRKHNVELLLNAPIVEVAADHIKLKDGRNIPTHTLIWTAGVKGTSDAADFGLES
                     ARGQRLIANEYMQAKGYEDKNIYIIGDLVFYEEFPGTPTPQIVQAAEQTGHTAAANIV
                     ASIKGGEKHKFKGNYQGFMVSIGAKWGVANLFDKIHLSGFLAIVMKHIVNLKYFFDIR
                     SGYYMFQYLMHEFFHIKDDRNVTRGHSSRYGNVLWSVPLRIFYGLVWFVEASKKIIGN
                     GEVFKPSTWFGEGSWFTGNSVFPWTWEYNVTDVASGASAAGDATTAASGAGASAGEAA
                     TQAAHFGLSYAYGEEPMAVIEKSPDWFTKIMQVIMPNNDVAMFFQKFMVFFEIALALA
                     LIAGLFTWLCSATTIVLVITFCLSGMFYWVNIWFIFVAFALMNGSGRAIGLDRWVIPW
                     LQRTLGNWWYGRPSSQYGKK"
     gene            5014..5430
                     /locus_tag="IWO89_002457"
     CDS             5014..5430
                     /locus_tag="IWO89_002457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NusG domain II-containing protein"
                     /protein_id="HAQ0457172.1"
                     /translation="MSLKEFAEKSLLKPWDVFIILLLTVLSFLPVMIFGLQQNTATAE
                     KEAVLRVDGTEIKTFPLMAGEESNTYTYKDEHGDYNLIEIDGDRVRIKEADCDDQICV
                     RRGWATKNGETIVCLPHKLVIEVRSTDGGDEDSLIY"
     gene            5444..6010
                     /locus_tag="IWO89_002458"
     CDS             5444..6010
                     /locus_tag="IWO89_002458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gx transporter family protein"
                     /protein_id="HAQ0457173.1"
                     /translation="MSKLQKMIYISLLVAQGVIIGLLENMIPYPFSFAPGAKLGLANL
                     ITIVAIFTMKKQDSFLLLWLRLFLTTLLGGTISTFLYSMSGALLSYFGMLLVKQLGPK
                     RVSIIGISAAGGFMHNVGQLLMASFIAQSWTVMLYLPVLSVLGILSGIAIGIAANYLL
                     RHVQTLQRFQKDYEQGTKQKWSPMFTKK"
     gene            6033..7019
                     /locus_tag="IWO89_002459"
     CDS             6033..7019
                     /locus_tag="IWO89_002459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HAQ0457174.1"
                     /translation="MKLHPMWENYPALSKELTETLKRMEETVRLKNKPVEQAIKETIH
                     AGGKLLRPAYQLLFSQFGPEQDRDKAIALAASIEMLHTATLIHDDIVDEADLRRGQPN
                     IRSQFGNVVSVYAGDYLFVCCFKLLSDYSTSLKSLQLNSRSMEKVLNGELGQMDDRYN
                     YELSVKEYLENISGKTAELFQLSCSVGAFESGTSERFAKKAGDIGLSIGMAFQIIDDI
                     LDYTKESQEIGKPVLEDMRQGVYSLPLIYSLQKNKPKLLPYLEKKAAMTEEDVDAVRK
                     IVEHTGGVEEARKLAASYTEKALKEIKKLPATSLRTKENLFSLTQLILDRKD"
     gene            7294..>8576
                     /locus_tag="IWO89_002460"
     CDS             7294..>8576
                     /locus_tag="IWO89_002460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HAQ0457175.1"
                     /translation="MSYSEFTKELLDILDLNLTFHEDAFRKERINDETCFVFDGTLTY
                     QPEECFHCHYQNKQTIIKWGWKKVSILLNDVSNYKTIVRINKQRFKCKHCGKTFLAED
                     SVSDRRCSIARRVKQAILELLSEPISMSLIARMKHISPTTVIRILRSLRPKTVSLNQP
                     LPEVVCFDEFKSVKNVSGAMSFVMMDGKTHQLIDIVENRQLNPLRDYFLRYPRKVREQ
                     VRLVVSDFYTPYRTLVKECFPNARIVADRFHISQHIGRAFTNHRIQVMKTFKKGDRRS
                     KHLKKYWRLLQKNAWELNGQHRYWRPSFRDHLTEAEIVDRLLYYDDSLKRGYEVYQVF
                     LSAIRRQDVPEFVALLKEDYKELPEHYQPVFTTFKKYRTEIKRALRVPYSNGPIECLN
                     NHIKVLKRIAYGFRNFQNYRERIFLYRGKYFKKTK"
BASE COUNT         2525 a         1688 c         1745 g         2618 t
ORIGIN      
        1 attattgata gcggtagtcg ctaaatcaaa aactagcatt ccaataaaaa ataaaaaagt
       61 gtttagccaa tgtatctcat gaaaaaaagc gatagagaat aataccccta ccgcaaaagg
      121 aaatacacta gccacctttg ttcgtatttc aactacttct aaaaagattt ttagtgtcat
      181 ggttcgtact ccttatttta gttcagaacg gtcacccatc gtgtaatggg agtcctccga
      241 taatttgaag gtgtctttga ttggatcggt cacatatact ttatcgtctt ttgtcacgaa
      301 gatcgcttcc gtcccgttat tcaattcgct ttcgacgtat tccagtcctt ttttcacacc
      361 cattgaaaag actgctgtag acaacccgtc accatcaatt gatttatctg ttatgatcgt
      421 gacacttgcg atatcattgt cgaacggata acccgttttt ggatcaaaca aatgatggta
      481 ttttttgcca tctacttcta aataacgctc gtaaattcca gaggtaacta atgttttgtt
      541 ccgttcttta atagaaccta aaacagatcc gcgagccatg ttcggatctt ggatgccgac
      601 tgtccagtct tggttttcgc ctcttgggct gtggcctaag acatagacat taccgcccaa
      661 gtcaacgatg gcagtggtca ctccttgttt tttcaagact ttcactactt cgtccgtaat
      721 gtaacctttg gcgattgcac ctaaatcgat gatcattcct ttttctttta gataaacagt
      781 tttttctttg tcatttagtt cgatcttatg ataatccact aatttcaatg cctgatcgat
      841 ttcttcttgg gaaggttttc tggcgtcatc aaaaccgatt cgccataatt gtgtgattgc
      901 accaatcgcc atatcgaatc cgccttgaga atcttggcta tattcgtagg cccgcttgac
      961 taacgtgtag acgtcatcag aaacttttac tggtttgatc ccagcttgct cattcacttc
     1021 atcgatctca gaaccttttt gattgatcgt gattttatca ccaagttctt ttactcgatc
     1081 gaaggcaggt ttcaatgctg attctttgcc ttcatcatac acacggatac gaacgtaagt
     1141 ccctaacaag aactgttcat cagaatatgg atctttcaat atcttggtag ctgctgaact
     1201 ttcagtggtt gttttatttg ctgagttgct gcagccagtt agcaccataa aagagatgcc
     1261 cagtgtgatt aaggaaaata gtagtttttt ccgcatcttt ttcctctctt cattttgaaa
     1321 ggtgagacga ctgcctaatc tggctatcgt cccaccctcc aacttttatt agtatgatta
     1381 tgtgattatt tttctgttac gatattatct acttcgattg tttgagtgtc gcctttttca
     1441 gctgcattca ctaattgttg tgcgtacatg atgaatgaat gtgagctgtg tgtagcacct
     1501 gtaacaactt ctacattagc aggtgagcca tcttcttcgc ccattgcttt gacgaattcg
     1561 tcgttcagag tagggatgta agtttttggt tctgtaccgc tttttgcttt catattttca
     1621 ttatattctg tatcatcttg ttttgattta ccgtctttgt cgatataatc aaagtttgat
     1681 tcagttactt tgccgccagc aactgtcatt tcgaattgca cacggtagcc gttagagtag
     1741 ttcttttctt caagcttgta agtgccgtct ttcaaatcag caccattgtc gatttcgatt
     1801 gtatctgtgt taccagcttg agcagcttgg atcaattgtt gtgcgtagtt ttggaagctt
     1861 tcagatgagt gagtagctcc agttacgact tccacaccgc ttgcgctttg tgcttttaag
     1921 aattggtcat tcaactcagg aatgtatttt tctgggtttg tgccagattt tgcttccatg
     1981 tttttgttgt attctgcatc ttttgttttt gattcgccgt tttcattgac gttatcatat
     2041 tttgattcag tgattttgcc gtcttttaca accatttcaa agactgcacg gtaaccattt
     2101 gaataatttt tttcttctaa tttatatgtg ccgtcttgca aatctccgcc agctacaact
     2161 tttgcagatg attctgttgt gctttttgca gtcgaagatt gtgctgtaga tgattcagaa
     2221 gaagagtttg agcttccgtt gtcatctgcg ccgcaagcgc ctaacactag agctgaaaat
     2281 gctaatacgg caaatccagt tacaaaacgt tttttcttca ttttaatacc ccaccattcc
     2341 atttctagtc cacaattttt ctttgtgatt cattatacat tctaatcttt ttagcctata
     2401 agtcaactat ttttgataaa taacccatgt aatatttcaa acgttttctt tcagcaaatt
     2461 tcgtgaaaaa acgctttatt ttaaaatttg aaaaaaatcc aaaaggagtt agacaaaaga
     2521 acagaacatc tatataatat atggagattg tgtaataagt atcaaactaa aagaattatc
     2581 atatattcat tatatagata gtctataagg agcggatgat caatggcaaa gcaaaatatt
     2641 gtcgttgtag gagcaggata tgccggtgtt tcagctacaa aatacttggc aaagaaactg
     2701 aaaaaagaag atgtagcaat tactctgatc gatcgtcatt cgtaccatac tatgatgacc
     2761 gagttgcatg aagtagctgg cggacgtgta gaaccagaag caatccaata cgatctgcag
     2821 catttgtttg ctcgtcaaaa gaatgtacaa cttgtcacag acactgtaat cggtatcgat
     2881 aaagaacgaa aaatcgtcaa gaccaaatta ggttcctatc cttttgatca gctgatcctt
     2941 ggaatgggcg gagagccgaa tgactttggt acacctggtg tcaaagcaca cggattcacc
     3001 ctttggtcat ttgaagattc tgtaaggatt cgggaacata ttgagaagac agtagcaaaa
     3061 gctgcattgg aaccagatga tgaactgcgt aaagccatgc tgactttcgt tgtctgtggt
     3121 tctggattta ccggtattga aatgattggg gaactgatcg actggaaaaa tcgcctagcc
     3181 aaagattata aattagatcc aaacgaaatc acactgatgg tcgtagaggc aatgccgacg
     3241 atcttgaata tgttggatcg aaatgatgct gcaaaagctg aacgctattt gcgtaagcac
     3301 aacgtggaat tattattgaa tgcaccaatc gtagaagtgg ctgctgatca tatcaaattg
     3361 aaagacggac gcaacattcc gactcacaca ctgatttgga cagctggtgt caaaggaact
     3421 tctgacgctg ctgactttgg attagaatca gcacgcggac agcggttgat cgccaatgaa
     3481 tatatgcagg caaaaggcta cgaagataaa aacatctata tcattggtga tttagtcttt
     3541 tatgaagaat tcccaggaac accgacaccg cagatcgttc aagctgctga acaaacagga
     3601 cacacagccg cagcgaatat cgtggcttcg atcaaaggcg gagaaaagca taaatttaaa
     3661 ggaaactatc aaggatttat ggtttctatt ggtgccaaat ggggggtagc taacctattt
     3721 gataagatcc atttaagcgg atttttagct atcgtaatga aacatatcgt gaatttgaaa
     3781 tacttctttg atattcgttc tggttattat atgttccaat atttaatgca tgagtttttc
     3841 catatcaaag atgatcgcaa cgtgactcga ggacattctt ctcgttacgg caatgtctta
     3901 tggagtgtgc cacttcgtat tttctatggt ttagtttggt tcgttgaagc ttccaaaaaa
     3961 atcattggga atggtgaagt gttcaaacca agtacttggt ttggtgaagg atcatggttc
     4021 actggaaaca gtgtgttccc ttggacatgg gaatacaatg taacagatgt tgccagtggt
     4081 gcttctgctg ctggtgatgc aaccacagct gccagcggag caggggcaag tgccggagaa
     4141 gcagcgactc aagctgcaca ttttggtttg agttatgcat acggtgaaga accaatggct
     4201 gtgattgaga agtcaccaga ctggttcaca aagatcatgc aagtgatcat gccaaacaat
     4261 gatgtagcaa tgttcttcca aaaattcatg gtattctttg aaattgcctt ggcattagca
     4321 ttgattgctg gattattcac atggctatgc agtgcgacaa cgattgtatt ggtgatcacg
     4381 ttctgcttat caggcatgtt ttactgggta aacatttggt tcatttttgt tgctttcgca
     4441 ttgatgaacg gttccggacg tgcgattggg ttggatcgct gggtaattcc atggctacaa
     4501 cgtacactag gtaattggtg gtacggtaga ccaagttccc agtacggcaa gaaataaata
     4561 gataaagaac gttatttgaa tgcaagattt caatagaaag gggctgagac aaaccgtcac
     4621 agcctccttt ttatgtaaaa ttaaaggaat aagatgcccg agataagcct ccatgagcaa
     4681 gacacaggtg tcacaacctc tgttacaacc tcttgttata aacggtgttc cagaagtagc
     4741 ttcttcgaaa aataagccga aatttcacaa aaacttgaaa agtaattttc gtaaattcct
     4801 tcttatttct tgaagttgcc cacttctgtc acaacctctt gttataaacg gtgttccacc
     4861 tgctgcttct cggtaataag cccaaagaat tagaaaacat ggaaagctgt tttctattct
     4921 tcgttcttat tactcaaagc aagacacagg tgtcacaacc tcttgttttt atgctaaaat
     4981 tgggtgagtt aatctggaaa aaggagaaca aaaatgagcc ttaaagaatt tgctgaaaaa
     5041 agtctgctca aaccttggga tgtttttatt attttgcttt tgactgttct ttctttttta
     5101 ccagtcatga tttttggtct tcagcaaaac accgcaacag cagaaaaaga agcggtactg
     5161 cgtgtggatg gcacagaaat caaaacattc cctttgatgg ctggagaaga gagcaacaca
     5221 tatacttaca aagatgaaca cggagattat aacttgatcg agatagatgg cgatcgagta
     5281 cggataaaag aagcagattg cgatgaccag atctgcgtac gtcgaggctg ggcaacgaaa
     5341 aatggcgaaa ccatcgtctg tttgccacat aaattagtga tcgaagtccg ttcaacagat
     5401 ggaggtgacg aagacagtct gatttattag accttcacac accatgagta aattacaaaa
     5461 aatgatttat atttcgctac ttgttgcaca aggagtgatc attggtttat tggaaaatat
     5521 gatcccttat cctttttctt ttgcgcctgg tgcaaaacta ggcttagcca atctaatcac
     5581 gatcgttgcc atttttacca tgaaaaaaca agacagcttc ttactacttt ggctacgact
     5641 attcctgact actttgctag gcggaacgat ttccacattc ctttatagta tgagtggtgc
     5701 tttgcttagt tattttggaa tgttattggt caagcaatta ggaccaaaac gtgtcagtat
     5761 catcggaatt agtgcggccg gcggtttcat gcataatgtc ggacagcttt tgatggcttc
     5821 tttcattgca caatcatgga cagtcatgtt gtacttgcca gtcctttcag ttttagggat
     5881 tttatcaggg attgcaatcg ggatcgcagc gaattatctt ttacgacatg tgcagacact
     5941 tcaacgattc caaaaagatt atgagcaggg aaccaaacaa aaatggagcc caatgtttac
     6001 caaaaaataa tcgtcacaca aggagttttt ttatgaagct acatccaatg tgggaaaact
     6061 atcctgcttt aagtaaagaa ttgacagaaa cactaaaacg gatggaagaa actgtccgcc
     6121 tgaaaaacaa acctgtcgaa caagccatca aagaaacgat ccatgccggc ggaaagctgt
     6181 tgcgtcctgc ctatcagcta ttgttctcac aatttggacc agagcaagat cgagacaaag
     6241 ccatcgcttt ggcagcttct atcgaaatgc tccatacggc cacactgatc catgatgata
     6301 tcgttgatga agcggatctg cgacgtggtc agcctaatat ccgtagtcag tttggtaatg
     6361 tcgtttctgt ctatgcagga gactatttat ttgtctgctg ttttaaacta ttgtctgatt
     6421 actccacttc cttgaaaagt ctccaattga attctcgcag tatggaaaaa gtattgaatg
     6481 gcgaattagg gcagatggat gatcgctata attatgaact ttctgtaaaa gaatatttgg
     6541 aaaatatttc aggtaagaca gctgaactgt tccaattaag ttgttctgtt ggcgcttttg
     6601 aaagtggaac aagcgagcgc ttcgcaaaaa aagcaggaga tatcggactt tctatcggga
     6661 tggcctttca gattatcgat gatattttag attatacaaa agagagccag gaaatcggca
     6721 aacctgtact agaagacatg cgtcaagggg tctattcttt accgttgatc tactctttgc
     6781 aaaaaaacaa accaaagcta ctaccttatt tagagaagaa agcggcaatg acagaagaag
     6841 atgtcgacgc cgtacgcaaa atcgttgaac atacaggagg cgtggaagaa gctaggaagt
     6901 tagcggcctc ttacacagaa aaggctttga aggaaataaa gaaattacct gccacctctc
     6961 ttcgaacaaa agagaatctc ttttccctta cccaactgat tttggatcgc aaagactgaa
     7021 tagggaaagg ttcgatacgt aaataaattt ctcagaagga aatctacgtg ttttttgaca
     7081 aattgaattg tagttcttgt atttatgata aaaagcaaaa ataaagagca acgtccgtta
     7141 gcgacgttgc tcttttttat cggatctata atatctagtg ttggtgacgc cttctgttgc
     7201 caatactttt tcttttttct aataaacgca aacagacacc ttgaaatcct ttagaataaa
     7261 gtcgacgaaa accattcaaa ggagtgtttc aagatgtcct attcagagtt taccaaagag
     7321 ttactagata ttctagacct aaatcttacc tttcatgaag atgcctttcg caaagagcgc
     7381 attaatgacg aaacatgttt tgtttttgat ggcacattga cctatcaacc agaggaatgc
     7441 ttccattgcc actatcaaaa taagcaaacg atcattaaat ggggctggaa aaaagtatcc
     7501 atcttattga atgatgtcag taactacaag accattgtgc ggattaacaa acagcgcttc
     7561 aaatgtaaac actgtggaaa gactttttta gcagaagatt cagttagcga tcgtcgctgc
     7621 tccattgccc gcagagtcaa acaagccatt cttgagctat taagtgagcc tatttctatg
     7681 tctttgattg cacgtatgaa gcacatttct ccgaccactg tcattcgaat acttcgcagc
     7741 cttcggccta aaaccgtttc tttgaaccag ccgttaccag aagtggtgtg tttcgatgaa
     7801 ttcaaatcgg tgaaaaacgt ctcgggagcc atgagctttg tcatgatgga cggcaagacc
     7861 catcaactga ttgatattgt ggaaaatcgg caactcaatc ctttacgcga ctatttcttg
     7921 cgctacccta gaaaagtccg tgaacaagta cgtctagtgg tttcagattt ctatacaccc
     7981 tatcgcacat tggtgaaaga gtgttttccg aatgcccgaa tcgtcgccga tcgctttcat
     8041 attagccaac acatcggtcg ggcctttacc aaccaccgaa ttcaagtaat gaagaccttt
     8101 aaaaaagggg atcgtcggtc gaagcactta aagaaatact ggagactact tcaaaagaat
     8161 gcgtgggaat tgaatggtca gcatcgctac tggcgtccgt catttcgaga tcatctgaca
     8221 gaagcggaga tagtcgatcg gctattatat tatgatgact ccttaaagcg aggctacgaa
     8281 gtctaccagg tttttctttc cgctatcaga agacaagatg ttcctgaatt cgtcgcgctc
     8341 ctaaaagaag actacaagga gttgccagag cactatcaac cagtgttcac gaccttcaag
     8401 aaatatcgaa cagaaatcaa acgagcgttg cgagttccct attccaacgg ccctatagaa
     8461 tgtttgaaca accatatcaa agtactaaaa cgaattgcct atggctttag aaactttcaa
     8521 aactaccgag aacgaatctt tttatatcga ggaaaatatt tcaagaaaac taagaa
//
LOCUS       DACEEZ010000099         8493 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20313_2017
            SAMN12639966-rid9879223.denovo.099, whole genome shotgun sequence.
ACCESSION   DACEEZ010000099 DACEEZ010000000
VERSION     DACEEZ010000099.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639966
            Sequence Read Archive: SRR10023158
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8493)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8493)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741683.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741683.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:44:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,016
            CDSs (total)                      :: 2,962
            Genes (coding)                    :: 2,889
            CDSs (with protein)               :: 2,889
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 36 of 73
            Pseudo Genes (incomplete)         :: 29 of 73
            Pseudo Genes (internal stop)      :: 18 of 73
            Pseudo Genes (multiple problems)  :: 8 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8493
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639966-rid9879223.denovo.099"
                     /isolate="E20313_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(<1..173)
                     /locus_tag="IWO89_002461"
     CDS             complement(<1..173)
                     /locus_tag="IWO89_002461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009325336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAQ0457176.1"
                     /translation="MATKIADELENHVLETGMQKITTHASITAKPFFEKRGYKVINEQ
                     TVELRGQLFTNFL"
     gene            complement(593..1714)
                     /locus_tag="IWO89_002462"
     CDS             complement(593..1714)
                     /locus_tag="IWO89_002462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010730282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS4 family transposase"
                     /protein_id="HAQ0457177.1"
                     /translation="MDKYNVKTSFQKWFSAINFSGLSENSQSLISDFDFYSKKLDFQT
                     TLKVLLHAVYEELPSYREIDRAFLDQRLCKELGIDSLCYSSLSRRAPEIKQEVLMEIF
                     TQLVARISAQQPSSKTTSLQLIDSTTIPLNKAWFPWAKFRKTKSGIKLHLNLCYLDKT
                     NQYPESFTMTNASEHDRNHLEVLVDKTQATYVVDRGYFDYKLLDKLNRDGYFFVTRTK
                     SNTKITILDQIEVADTTTRDGTIISDQQVILVGGVNHVTERFRLVTVLTKGQKILRMV
                     TNLFDVSPNEVADMYQARWQIELLFKHLKQNLTIKRLYSHSEQGAINQVILTLIATLL
                     TYVIKIELNTTATLFQLKRTFHYLMFEPAEEWFKRYRPG"
     gene            2042..3451
                     /locus_tag="IWO89_002463"
     CDS             2042..3451
                     /locus_tag="IWO89_002463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS21 family transposase"
                     /protein_id="HAQ0457178.1"
                     /translation="MDDIKTIKHLRNNRDQSIDQIRKAMNINWRTAKKYADSDQLFIT
                     DPTRKNKSMMDDGWGEIIGSWLEEDLRLRRKLRRTAKAIHEQLKPLGFPGSYRTVCVY
                     ISQWKKQRYDETNYQAVDRLDHPIGEAQVDFGTMEVMHEDHMKDIQLLIMSFPYSNAT
                     SASAMPAQNQECFLEALKQLFNQCGGVPKTIRIDNLTPAVKKTKSKFEEAQLTDEFLR
                     FTIHYGCDVQVCNPRSGHEKGHVENKVGYVRYNFFSTSPIMDSFESLNKQLEEQLKAD
                     RKRTHYSKHQLIEDLWKKENKALHSLPQEDYPIFKEVEAKLNRYHEMKLDQTVIHIPK
                     AYGYSQLTVVLTWNKFKVLTPHGEILMNEYRPYMNKKRLIPWSDIMKQWSNKLNVVSY
                     SRYWKYLPARLQNYLAIDNNHLKYRRIKQLLSLLATYDIQQINEQFYELVVQEDAPSD
                     FDVNWSNYDALTKTGGVQK"
     gene            3448..4176
                     /locus_tag="IWO89_002464"
     CDS             3448..4176
                     /locus_tag="IWO89_002464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HAQ0457179.1"
                     /translation="MIGQTLEDYCKKLRLSHIITNIPRASFEAPEQFITDLFRLELET
                     RQQIKLERMEKLAKFPVPKSLKNYEWHEDIFLPSHLDKETLVSLDFLKRQENIVCVGS
                     PGTGKTHLAIALGRKACSEGVSTKFFRVNNLVEQLENALKKQQLASFKKRFETTELII
                     LDEMGYLPLTKEGAELLFQLISDWYEQKSIIITSNLEFSQWNRVFMDSRLTAALVDRL
                     IHHAHIISFKGESFRLKNSLSKSY"
     gene            complement(4304..5215)
                     /locus_tag="IWO89_002465"
     CDS             complement(4304..5215)
                     /locus_tag="IWO89_002465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HAQ0457180.1"
                     /translation="MKIKIERSNKEKIPKKKKERVVSVGKHRKMVLALWLLLSCSLAF
                     GIYKNFTAIDQHTTHEKVVVKEKIVNTSGVENFTVDFVKEYFSWKNNKEVIEKRMTNL
                     EQYLTEEGLALSQDMVRADIPTSSEVVSVKILDVEKHSEEFVVSFVVDQKITEGKKTQ
                     SISSAYRLMIFEDEKGNHIVTSLPTMIGKPEKAKHKVRQVKSDSKIDSKTTEEITEFL
                     ETFFRLYPAASEKELEHYVEKDAMQPINTNLKFVELVNPIYKQKGKAIQVIVGVEYLD
                     ELSKTTSVFQYRLKLYKNENWTISNCE"
     gene            complement(5232..6239)
                     /locus_tag="IWO89_002466"
     CDS             complement(5232..6239)
                     /locus_tag="IWO89_002466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HAQ0457181.1"
                     /translation="MNLKKLSIISLTFLTISFVGILMCVGLLFGEDGEGSGSSGTSPG
                     GSSVSEVVLKHRSMVEKYAKESDITEYVSILLAIIQVESGGTMEDVMQSSESAGLSPN
                     SLSTEASIKQVCLYFSSLVKCSKELGCDQDSIIQAYNYGDGYLDYVAKNGKKHSFALA
                     ESFSKEKSGGQKVDYPNPIAIKENSGWRYNYGNMFYCLLVKQYLTTTQFDDKTVQGIF
                     DEAFKYEGTAYVFGGSSPGTGFDCSGLTQWCYAKVGLKLPRVAQDQYDAMTHIDLKDA
                     KPGDLIFFHSTYDAGTYVTHVGIYAGENRMFHAGDPVGWTNLTDSYWQQHIIGAGRYK
                     Q"
     gene            complement(6236..8365)
                     /locus_tag="IWO89_002467"
     CDS             complement(6236..8365)
                     /locus_tag="IWO89_002467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HAQ0457182.1"
                     /translation="MKKKLFRIVGIVFISIVTLTVLLSLVGTVAEATGLVDDTVEAGN
                     LYSKYSLNNYQLDFFVDSSWDWLPWNWGDGLGKSVMYGLYAITNFIWTVSLYLSNATG
                     YVVQEAYKLDFISDTAKSIGKNIQTLAGITENGLQTSGFYFGFLLLMILALGVYVAYT
                     GLLKRETTKAVRTVINFVMIFLLSGSFIAYAPTYITKINDFSSDVSEAALSLGTKIVV
                     PNSESQGKDSVDLIRDSLFSIQVQQPWLLLQFDDSNIEEIGEGRVNKILSVSPDENKG
                     KDREEAVKAEIEDNDNANLSITKTMNRLGTVVFLVLFNIGISFFVFLLTGIMLFSQIL
                     FIIFAMFLPISFLLSMLPTYESLGKRAIIRLFNTIMMRAGVTLVITTAFSISTMFFNI
                     SATYPFFMVAFLQIVTFAGIYFKLGDIMSMFNLQSNDSQSMGRRVMRKPQMLMNRKLR
                     QLNRNVGRTFAFGGGAAVGGKLATSSTKGKGSSKINRVSNRKNHSARSINELNSDLKK
                     QEQPVGTKKESRINLVGRKVGKVLDTKEIVKDKAKQAKNQVTDAPTNLKYSLHKGMEK
                     TKKVPEDFKRGPFEEKANRGELREKQRQRRDAKMDEKRKVLNEAWNRHEKGRENVPIK
                     KEIKPQKNELPENRSLKPRVQVHSNPEIKRKLSSQEVIPNGSTQPLSKNNSFQQVKQR
                     FTFPKRTNQQVQRKKNTRPKPKPGEKR"
     gene            complement(8370..8477)
                     /locus_tag="IWO89_002468"
     CDS             complement(8370..8477)
                     /locus_tag="IWO89_002468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0457183.1"
                     /translation="MQDLYGRVGVVQVHPVFEELFHAFDTRPPVQERME"
BASE COUNT         2598 a         1728 c         1487 g         2680 t
ORIGIN      
        1 attaaaaagt tagtaaataa ttgccctcta agttccacgg tctgttcatt aataacttta
       61 tatcctcgtt tttcaaaaaa tggctttgcg gtaatggaag cgtgggtagt gattttttgc
      121 ataccagttt ctaaaacatg gttttccaat tcatctgcaa ttttagtcgc aatacctttt
      181 ccttgatggc tatgtttttg tcacaacttc agtattcctg taacaatacg gatttcagta
      241 tacaacaaca gcttttgaaa agatacttgc aaaaggatta agatatctat gacctgctat
      301 gctcactgat gttgaaaaga atgttttatt aggattgagg taagcaagat agtgaatatg
      361 tccggaaagc ctgtaaaata aggcttttcg gacatatgtg aagttataaa aagatagcga
      421 aaaatctatt tagtatcact aatgttgcga acatatcgtc agaatagtct gacatttatt
      481 tcttgctaaa aatttcaaaa aagtcaagtg ctgacgcagc ggatgactat tttccagaaa
      541 attttagtat gtattcaaaa atcaaacgtg acaacagcta ttaactacat cctcatccag
      601 gtctgtaacg tttgaaccac tcttctgcgg gctcaaacat taagtaatgg aaggtacgtt
      661 tcaattgaaa cagcgtcgct gtcgtattca actcaatttt tatcacataa gtgagaagcg
      721 ttgcgattaa ggtgaggatg acttgattta tcgccccttg ttcactatgt gaatatagtc
      781 gtttaatggt cagattttgt tttaagtgtt tgaacaacaa ttcaatctgc caacgggctt
      841 gatacatgtc ggccacttcg tttggcgata catcaaagag attggtcacc attcgcagga
      901 ttttttgtcc cttggtaaga accgttacca acctaaaacg ctctgtcaca tggttgacac
      961 cgccgacaag aatcacttgt tggtcactga ttatcgtccc atcacgggtt gttgtatctg
     1021 ctacttcaat ttgatccaag atggtaattt ttgtattaga ttttgtccgt gtaacgaaga
     1081 aatagccgtc acgatttagt ttgtctaaca acttgtaatc aaagtagccg cgatccacca
     1141 catacgttgc ttgcgtttta tcaacaagaa cttcaaggtg attacggtca tgctcactcg
     1201 catttgtcat cgtaaaactt tcaggatact gattggtttt atcaagataa cagagattca
     1261 aatgtagctt gatgccagac tttgttttcc gaaacttggc ccatggaaac catgctttgt
     1321 tcaacggaat agtcgttgaa tcaatcaatt gtagactggt tgtttttgag cttggctgct
     1381 gagcagaaat tctggcgact aattgcgtaa aaatttccat caatacttct tgcttgattt
     1441 ctggcgctct acgagataac gagctgtaac acagggaatc aatgccaagt tccttgcaga
     1501 ggcgctgatc taaaaaggcg cgatcaatct cgcgatagct tggcaattct tcataaaccg
     1561 catgaagtaa cactttaagc gtcgtttgga agtctagttt tttactgtag aaatcaaagt
     1621 cgctaattaa gctttgagag ttttctgata aaccagaaaa atttattgca gaaaaccatt
     1681 tttgaaatga ggtttttacg ttatacttat ccatgcgtga gtccttttta ttggtcttgg
     1741 ataagtcatc cgagaatagt ataaaggact tttttgcgtt tggatagctt tgtattaggc
     1801 ttttgatggg ttaaaaagtc tttgcaacac tagtaattta gtatttatgt ataaattgaa
     1861 agatatttta ccaccactta gtcttcttaa aagtgatcca acattttgtt tgtggcagaa
     1921 tttaactaca cactaatgca gccgaaagta cacaactatg cagcagtttg actgcgaact
     1981 ggtttccttc catcataagt ttgaatgact tagtgatgga ggtacagaaa ggttgttaac
     2041 tttggatgat attaaaacaa taaaacattt gagaaataat cgtgatcagt caattgatca
     2101 gataagaaaa gcaatgaata tcaattggcg aacagcgaaa aaatacgccg atagtgatca
     2161 actattcatt acagatccca ctcgaaaaaa caaatccatg atggatgatg gttggggaga
     2221 aatcatcggt agttggctag aagaagacct acgtttacgc agaaaacttc gtagaacggc
     2281 caaagctatc catgaacaat tgaaaccttt gggtttccct ggctcctatc gaacagtttg
     2341 tgtatacatt tctcaatgga aaaagcagcg ttacgacgaa acgaattacc aagctgtcga
     2401 tcgtcttgat catccgatcg gagaagcaca ggtcgatttt ggaacaatgg aagttatgca
     2461 tgaagatcac atgaaagata tacaacttct tatcatgtca tttccctata gtaatgcgac
     2521 gtccgcaagt gccatgccag ctcaaaacca agagtgtttc ctggaagcat taaaacaact
     2581 atttaatcaa tgcggcggcg ttccaaaaac gatcagaata gataacttaa ctccagcggt
     2641 gaaaaaaaca aaatcaaaat ttgaagaagc acaactaacc gatgaattct tacgtttcac
     2701 cattcattat ggttgtgatg ttcaagtctg taacccacgt agtgggcatg aaaaaggaca
     2761 tgtcgaaaat aaagtgggtt atgtccgata caatttcttc tcgacttcgc caatcatgga
     2821 ctcttttgag tccctaaata agcaacttga agaacaatta aaagcagatc gaaaaaggac
     2881 gcattattca aaacatcaat taatcgaaga tttgtggaag aaagaaaata aagcgttaca
     2941 ttctcttcca caagaggatt acccaatttt taaagaagtg gaagctaaac taaatcgtta
     3001 tcacgaaatg aaattggatc aaacggttat tcatattcct aaagcatatg gctattctca
     3061 attgactgtt gttttgactt ggaataaatt caaagttctt acgcctcatg gagaaatatt
     3121 aatgaacgaa tatcgcccct atatgaacaa aaaaagattg atcccatgga gtgacatcat
     3181 gaaacaatgg agtaataaat tgaatgtcgt tagttattcg aggtattgga agtacttgcc
     3241 tgcacgcctt caaaattatc tagcgattga taataatcac ctgaaataca gacggataaa
     3301 acaattacta agtttattag cgacctacga tatccaacaa attaatgaac aattttacga
     3361 attagtcgtt caagaagacg ccccctctga ctttgatgtg aattggtcaa actatgatgc
     3421 attaacaaaa acaggaggtg tgcaaaaatg attggacaga cgttagaaga ttattgtaaa
     3481 aagcttcgat taagccatat catcacgaac ataccaagag cttctttcga agctccagaa
     3541 caatttatta ccgatttatt ccgtttagaa ctggagacca gacagcagat caaattagaa
     3601 cggatggaaa aattagccaa atttcctgta ccaaaatcgc taaagaacta tgaatggcac
     3661 gaagatattt ttctaccatc tcatctagat aaggagaccc ttgtctctct tgattttcta
     3721 aaaaggcaag aaaacattgt ttgtgttggc tctcctggta cagggaaaac acatctagcg
     3781 atcgcattag ggagaaaagc ctgtagtgaa ggtgtttcaa ccaaattttt ccgagttaac
     3841 aatctcgtag agcaattaga gaatgcatta aaaaaacaac agctagccag tttcaagaag
     3901 cgattcgaaa caacagaatt aattattctt gatgaaatgg ggtacttacc attgacaaaa
     3961 gaaggagctg aacttctctt ccaactgatt tcagattggt atgagcaaaa gagcattatt
     4021 attacctcta atttagaatt cagtcaatgg aatcgagtgt ttatggattc tcgattaaca
     4081 gctgctctcg ttgatcggct aatccatcac gctcacatta tcagcttcaa aggcgaaagt
     4141 ttcagattga aaaattcatt atctaaatca tattgaagga aaaaactagt tgcagcaaaa
     4201 ctctgcactt tcggctgcat ttttttgtaa aaagctattg cacaacacaa acatttgaac
     4261 gacgtaattc caaatgttgg attgctttta gataatcttt tatttattca caattgctaa
     4321 tagtccaatt ttcatttttg tatagtttta atctatactg aaaaacactt gttgttttgg
     4381 acaactcatc aagatactca acaccaacaa tcacttgaat cgctttaccc ttttgtttgt
     4441 aaataggatt cactaattcc acaaatttca aattagtatt aattggttgc atcgcgtctt
     4501 tttcaacata atgctctagt tctttttctg aggctgctgg ataaagcctg aaaaacgtct
     4561 ctaaaaactc ggtgatttct tccgttgtct ttgaatcaat ttttgaatca ctttttactt
     4621 gtcttacttt atgttttgcc ttttctggct taccaatcat agtgggtaaa ctcgttacaa
     4681 tatgattgcc tttttcatct tcaaagatca tcagacgata tgcagaagaa attgattgtg
     4741 tctttttccc ttctgtaatc ttttgatcta ccacaaatga gacgacaaat tcttccgagt
     4801 gtttttcaac atctagaatt tttactgata caacctcgga actagtggga atatccgctc
     4861 gaaccatatc ttggctcaaa gcgagccctt cttctgtaag gtactgctca agattagtca
     4921 ttctcttttc aattacctcc ttgttattct tccatgaaaa atattctttc acaaaatcta
     4981 cagtaaaatt ttctactcca gaggtattca ctattttctc tttcacgacc actttctcat
     5041 gagttgtatg ctgatcaatg gccgtgaagt ttttgtagat tccaaaagca aggctacaag
     5101 acaataaaag ccaaagggca agcaccattt ttcgatgctt gcccacggat actactcgtt
     5161 ctttcttctt ttttggaatc ttttccttgt tacttcgctc aatttttatc ttcattttcc
     5221 attcctcttt tctattgttt ataccgacca gcaccaataa tgtgctgttg ccaatagcta
     5281 tcggtaagat tggtccaacc caccggatct cctgcatgaa acattctgtt ctctcctgca
     5341 taaatgccca catgagtcac ataagtaccg gcatcataag ttgaatgaaa gaaaatcaaa
     5401 tcgcctggtt tggcatcttt taagtcaata tgagtcatag catcatattg atcctgagcc
     5461 acccgaggga gtttcaaacc aactttggca taacaccatt gagttaaacc actacaatca
     5521 aacccagttc cgggacttga cccaccaaat acataagccg ttccttcata cttgaacgcc
     5581 tcgtcaaaaa ttccttggac tgttttatca tcaaactgtg ttgtagttag gtactgcttc
     5641 actaataagc aataaaacat attgccatag ttataccgcc acccactatt ttccttaatc
     5701 gcgatgggat tgggataatc gactttttga ccacccgatt tttcttttga aaaagattcg
     5761 gctaaagcaa aactatgctt ctttccgttt ttcgcgacat aatccaaata cccgtcacca
     5821 tagttataag cctgaatgat actatcttga tcgcatccta gttctttgga acacttaacc
     5881 aaagacgaaa aatagagaca cacttgtttg atggatgcct cggtacttaa tgaattcggt
     5941 gataaacctg ctgattcaga actttgcatg acatcttcca ttgtacctcc agattcgact
     6001 tgaataatcg ccaaaagaat cgaaacatac tctgttatat ctgattcttt ggcatacttt
     6061 tccaccattg aacggtgttt caaaactact tctgagacac tactacctcc aggagaagtt
     6121 ccagaactac cacttccctc accgtcctca ccaaacaaca aacccacaca catcaagatg
     6181 cccacaaaag agatggtcag gaaggttaac gaaataatac tcaatttttt taagttcatc
     6241 ttttctcacc tggctttggt tttgggcgag tgttcttttt tctttgtact tgttgattgg
     6301 ttcgctttgg aaaagtgaat ctctgtttca cttgttgaaa ggaattgttc ttgcttagtg
     6361 gctgagtgct tccgtttgga atgacttctt gactagaaag tttccgtttg atttcaggat
     6421 tcgaatgtac ttgtaccctt ggttttaaag acctattttc tggtagttca tttttctgtg
     6481 gttttatctc cttttttatc ggaacatttt cccttccttt ctcatgacga ttccatgctt
     6541 cgttcaatac tttgcgtttt tcatccattt ttgcgtctct tcgttgtcgt tgtttttctc
     6601 gcaactcccc tcgatttgct ttttcttcga aaggccctcg cttaaagtcc tctggtactt
     6661 ttttcgtctt ttccatgcct ttatgaaggc tgtatttcaa gttagttggt gcatcggtta
     6721 cttgattttt tgcttgtttc gctttgtcct tcacgatttc ttttgtatcg agtacttttc
     6781 caacttttct accaactaaa tttattcgtg attctttctt cgtgcccact ggttgttctt
     6841 gttttttcag atcagaattt agctcgttaa tgcttctcgc ggaatggttc tttctattgg
     6901 acactctatt gatttttgaa ctacctttcc cttttgtaga actggtagct agtttcccac
     6961 caactgctgc accgccacca aaagcaaaag ttcgacccac atttcgatta agctgtcgca
     7021 attttcgatt catcaacatt tgcggtttcc gcatgactcg ccttcccatt gactgactat
     7081 cattactttg aaggttaaac atgctcataa tgtcacctaa tttgaaatag ataccggcaa
     7141 aggtcacaat ttgtagaaaa gctaccataa agaagggata ggtggccgaa atattgaaaa
     7201 acatcgtgga gatactgaat gcagtcgtaa tgactaaggt aactcccgct cgcatcataa
     7261 tcgtattgaa caaacgaata atcgcccttt tgcccaaact ctcatacgtc ggcaacatcg
     7321 acaataagaa actaatgggc agaaacatcg caaagataat gaaaaggatt tgtgagaaca
     7381 acataatccc tgtcagaaga aacacaaaaa aggaaatccc aatattaaac aacactaaaa
     7441 agaccacggt tcctaaacgg ttcatggtct tggtaatact gagattcgca ttgtcattat
     7501 cttcaatctc tgctttgact gcttcttccc gatccttacc cttattttca tccggactca
     7561 cggataaaat cttattaacc cgaccctcgc caatttcttc tatattggaa tcatcgaatt
     7621 gtaatagtaa ccagggttgt tgaacttgaa tcgaaaataa actgtctcga atcaaatcca
     7681 cactatcttt tccctggctt tctgaattag gtacaacaat cttggttcca aggctaagag
     7741 ccgcttcact aacgtctgag ctaaagtcat tgatttttgt aatataggtg ggcgcatacg
     7801 caatgaaaga acccgagagt agaaaaatca tcacaaaatt aatgacggtt cggactgctt
     7861 tggtagtttc tcgttttaat aatccggtat acgccacata aactcctaga gctaaaatca
     7921 tcaataatag gaacccaaag tagaatcccg aagtctgtaa tccattttca gtgataccgg
     7981 ctaaagtttg gatattcttt ccaatacttt tagccgtatc cgaaatgaaa tccaacttgt
     8041 aagcttcttg aaccacatag cccgtggcgt ttgataaata cagacttact gtccaaataa
     8101 aattggtaat ggcataaaga ccatacatca cgcttttccc taagccatcc ccccaattcc
     8161 aaggtaacca atcccaagaa ctatccacaa agaaatccag ttgataattg ttcagagaat
     8221 atttggaata aaggtttccg gcttcaactg tatcatcaac cagacctgtc gcctcagcta
     8281 ccgtaccgac taaactcaat agaactgtca gagtgacgat gctaataaaa acgattccca
     8341 caatccgaaa aagtttcttt ttcatcccct cactccattc tctcttgaac cggcggacga
     8401 gtgtcaaaag catggaagag ttcttcaaag actggatgaa cttgaaccac acctactcgc
     8461 ccatacaagt cttgcatcaa gcattgaccg ttt
//
LOCUS       DACEEZ010000100         8361 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20313_2017
            SAMN12639966-rid9879223.denovo.100, whole genome shotgun sequence.
ACCESSION   DACEEZ010000100 DACEEZ010000000
VERSION     DACEEZ010000100.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639966
            Sequence Read Archive: SRR10023158
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8361)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8361)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741683.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741683.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:44:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,016
            CDSs (total)                      :: 2,962
            Genes (coding)                    :: 2,889
            CDSs (with protein)               :: 2,889
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 36 of 73
            Pseudo Genes (incomplete)         :: 29 of 73
            Pseudo Genes (internal stop)      :: 18 of 73
            Pseudo Genes (multiple problems)  :: 8 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8361
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639966-rid9879223.denovo.100"
                     /isolate="E20313_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(114..473)
                     /gene="rplT"
                     /locus_tag="IWO89_002469"
     CDS             complement(114..473)
                     /gene="rplT"
                     /locus_tag="IWO89_002469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HAQ0457184.1"
                     /translation="MARVKGGTVTRKRRKRVLKLAKGYYGSKHTLFKTAKEQVMNSYN
                     YAYRDRRQKKRDFRKLWIARINAAARMNGLSYSKLMHGLKVAEIDINRKMLADLAVND
                     AAAFTALADQAKEALAK"
     gene            complement(526..726)
                     /gene="rpmI"
                     /locus_tag="IWO89_002470"
     CDS             complement(526..726)
                     /gene="rpmI"
                     /locus_tag="IWO89_002470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001125942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HAQ0457185.1"
                     /translation="MPKQKTHRGSAKRFKRTGKGGLKRFRAFTSHRFHGKTKKQRRQL
                     RKASMVSSGDFKRIRQQLAKMK"
     gene            complement(798..1322)
                     /gene="infC"
                     /locus_tag="IWO89_002471"
     CDS             complement(798..1322)
                     /gene="infC"
                     /locus_tag="IWO89_002471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HAQ0457186.1"
                     /translation="MTIAKDMMVNDGIRARELRLIGSDGEQLGVKTKAEALNIAEQAN
                     LDLVLVAPGAKPPVARIMDYGKYRFEQQKKDREARKKQKVINVKEVRLSPTIDLNDFN
                     TKLRNARKFLEKGDKVKASIRFKGRAITHKEIGQKVLNRLAEETADIATVEQKAKMDG
                     RSMFLTLAPKNDSK"
     gene            1642..2274
                     /locus_tag="IWO89_002472"
     CDS             1642..2274
                     /locus_tag="IWO89_002472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HAQ0457187.1"
                     /translation="MKKIEPIPPSLPLLTKGEFYLEDEEIFSGLLINGGDQSYLDAKN
                     MVLRESHIQKLTMQKTVLRHFECSNVIFEKCDFSNLEWIGGSFHQVVFHQCKLTGTNF
                     AESYLRDCTFTDCIASMASFSSTNLKAVSFDHCQLEDSEFYEVTWKNLFLSDNQLTGS
                     NWFRTSLKGLDFTTNFFTAIALSQDSLAGLIVNQEQALVIAQALGLQIKD"
     gene            complement(2276..3181)
                     /locus_tag="IWO89_002473"
     CDS             complement(2276..3181)
                     /locus_tag="IWO89_002473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HAQ0457188.1"
                     /translation="MREKHYFNQDRNFWINVQQNDVLAVKNLKEEYGISDEMLTYSLD
                     KNERARVEYDSFDEALLLVSNVPHQQKVDNHYETSPIAFILKEDGLFTFTTPNTEYVI
                     RLIRSLLERMPDMSVYSLLFRTLFLISDSFFPLIEEVNSERQRLNLKLREKTTNKNLL
                     QLSDLEIGLVYLVTGTKQNVVLLEQIKALAIYRKLSEKEKEELDDALIEARQAVEMTN
                     LASQILDQLSGTYNNLLNNNLNDTMKFLTVWSLILTVPTIVTGFFGMNLQLPFTHSVF
                     GWGIALIISLVLSIWMLIALWRRIR"
     gene            complement(3266..3919)
                     /locus_tag="IWO89_002474"
     CDS             complement(3266..3919)
                     /locus_tag="IWO89_002474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HAQ0457189.1"
                     /translation="MIVLAGTIGAGKSSLTEMMAEHFDSQAFYESIDDNEVLPLFYAN
                     PEQYAFLLQIYFLNKRFASIKQAMKDDNNVLDRSIYEDSLLFHLNADLGRATETEVRV
                     YDELLENMMEELPYAAHKKHPDLLVHIRVSFDTMLERIEKRGRSYEQLSFDPSLYDYY
                     KELNRRYDQWYEEYKESPKIQIDGDRYNFVEDPQAKEEVLKMIEEKLAEIRQTKVAI"
     gene            4241..5185
                     /gene="mvk"
                     /locus_tag="IWO89_002475"
     CDS             4241..5185
                     /gene="mvk"
                     /locus_tag="IWO89_002475"
                     /EC_number="2.7.1.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mevalonate kinase"
                     /protein_id="HAQ0457190.1"
                     /translation="MANYGQGESSGKIILMGEHAVVYGEPAIAFPFYATKVTAFLEEL
                     DAMDDQLVSSYYSGNLAEAPHALKNIKKLFIHLKKQHDIQKNLQLTIESTIPAERGMG
                     SSAAVATAVTRAFYDYLAFPLSREILLENVQLSEKIAHGNPSGIDAAATSSLQPIYFT
                     KGHPFDYFSLNIDAFLIVADTGIKGQTREAVKDVAHLFETQPHETGQMIQKLGYLTKQ
                     AKQAIIENSPETLAQTMDESQSLLEKLTISNDFLNLLIQTAKDTGALGAKLTGGGRGG
                     CMIALAQTKTKAQEISQALEDAGAAETWIQGLGVHTYV"
     gene            5178..6155
                     /gene="mvaD"
                     /locus_tag="IWO89_002476"
     CDS             5178..6155
                     /gene="mvaD"
                     /locus_tag="IWO89_002476"
                     /EC_number="4.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diphosphomevalonate decarboxylase"
                     /protein_id="HAQ0457191.1"
                     /translation="MFKGKARAYTNIALIKYWGKKNEELILPMNNSLSLTLDAFYTET
                     EVIFSDSYMVDEFYLDGTLQDEKATKKVSQFLDLFRKEAGLSLKASVISQNFVPTAAG
                     LASSASGLAALAGACNTALKLGLDDLSLSRFARRGSGSACRSIFGGFVEWEKGHDDLS
                     SYAKPVPSDSFEDDLAMVFVLINDQKKEVSSRNGMRRTVETSSFYQGWLDSVEGDLYQ
                     LKQAIKTKDFQLLGETMERNGLKMHGTTLAAQPPFTYWSPDSLKAMDAVRQLRKQGIP
                     CYFTMDAGPNVKVLVENSHLSEVQETFTKLFSKEQVITAHAGPGIAIIE"
     gene            6168..7253
                     /locus_tag="IWO89_002477"
     CDS             6168..7253
                     /locus_tag="IWO89_002477"
                     /EC_number="2.7.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomevalonate kinase"
                     /protein_id="HAQ0457192.1"
                     /translation="MIEVSAPGKLYIAGEYAVVETGHPAVIAAVDQFVTVTVESARKV
                     GSIQSAQYSGMPVRWTRRNGELVLDIRENPFHYILAAIRLTEKYAQEKNILLSFYDLK
                     VTSELDSSNGRKYGLGSSGAVTVATVKALNVFYALNLSQLEIFKIAALANLAVQDNGS
                     CGDIAASCYGGWIAFSTFDHPWLQEQETQHSISELLALDWPGLSIEPLIAPEDLRLLI
                     GWTGSPASTSDLVDQVHRSREDKMVAYQLFLKNSTECVNEMIKGFKENNVTLIQQMIR
                     KNRQLLHDLSAITGVVIETPALNKLCNLAEQYEGAAKSSGAGGGDCGIVIVDQKSGIL
                     PLMSAWEKAEITPLPLHVYSDQRKENR"
     gene            7250..8314
                     /locus_tag="IWO89_002478"
     CDS             7250..8314
                     /locus_tag="IWO89_002478"
                     /EC_number="5.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 2 isopentenyl-diphosphate Delta-isomerase"
                     /protein_id="HAQ0457193.1"
                     /translation="MNRKDEHVSLAKAFHDKQKNEFDFVRIIHNPLPQIAVSDVDLST
                     QAVGFTLSSPFYINAMTGGSEKTKKINQDLAIVAREADLMIATGSVSAALKVPSLADT
                     YTIMRQEYPHGKIIANIGAGTSVERAKEAIRLFHADALQIHLNAPQELVMPEGDRDFT
                     NWKVLIQETQTAIDVPLIVKEVGFGMTRETLNDLAALGVHTVDISGRSGTSFTQIENA
                     RRSKRELSYLADWGQSTVSSLLEANEADTSMEILASGGIRNAYDIFKALCLGANAVGT
                     SGTVLTHLMNHGVEETIILMKQWQEELRLLYTMVGATNTAALHQQSLIFSGPVKDWCE
                     ARGIDLVKYGRRTEKSVGQK"
BASE COUNT         2488 a         1720 c         1647 g         2506 t
ORIGIN      
        1 tattttagac ttatacgtta ttgtcctgtt tcatttgatg gaggattgta aataaaaaaa
       61 gaggaaagaa cataactttt cgttatcttc tttcctcttt ataattgttt agcttattta
      121 gccaaagctt cttttgcttg gtcagccaat gcagtaaatg ctgctgcatc gttaacagcc
      181 aagtcagcta acattttacg gttgatgtcg atttcagcaa ctttcaaacc gtgcattaat
      241 tttgagtagc tcaagccatt catacgagct gctgcgttga tacgagcaat ccacaattta
      301 cggaagtcgc gtttcttttg acgacgatcg cgatatgcgt agttgtatga attcattact
      361 tgttcttttg ctgttttaaa taaagtgtgt tttgaaccgt agtaaccttt tgctaatttt
      421 agcactcttt tacgacgttt acgagttact gttccacctt taacacgtgc catgtataat
      481 tcctcctaat aaaaataaat cttcagattc tgttatttgt acttcttatt tcatttttgc
      541 tagttgttgg cgaatacgtt tgaagtcgcc actagaaacc atgcttgctt tacgcaattg
      601 acggcgttgt ttttttgttt tgccgtggaa acggtgactt gtaaacgcgc ggaagcgttt
      661 caatccgcct ttaccagtac gtttgaaacg ttttgctgat ccgcggtgtg ttttttgttt
      721 tggcatgact atatttcctc ctcaaaatct ttcgttatcc gactatcggc tgtccaacag
      781 ccagttagtc attcaaatta cttactgtcg tttttcggtg ccagcgtcaa gaacatgctg
      841 cgtccgtcca ttttcgcttt ttgttccact gttgcaatat ccgcagtttc ttcagctaag
      901 cgattcaaga ctttttgacc aatttcttta tgggtaatgg cacggccttt gaaacggata
      961 gaagctttca ctttatcgcc cttttccaag aacttacgtg cattacgaag ttttgtattg
     1021 aagtcattca agtcgatcgt aggacttaag cgaacttctt ttacattgat cactttttgt
     1081 tttttacgcg cttcgcggtc tttcttttgt tgttcgaaac ggtatttacc gtagtccatg
     1141 attcgcgcaa ctggtggttt cgcaccaggg gcaactaaca caagatccaa gtttgcttgt
     1201 tctgcaatat tcaatgcttc tgcttttgtc ttaactccta attgctcacc atctgaaccg
     1261 atcaatcgta actctcgtgc acgaatgccg tcgttaacca tcatatcctt tgctatggtc
     1321 attcacctcc aaatattatg agagaaagaa aagcttaccg aataaacggc aataaaaaaa
     1381 cgagttctct tacagaaccc gcgcgtactt cacctaatag aaccagtgaa aactatctag
     1441 cccagtgaca ttatcaacta aggcgagaag cgggtgcttc tgcttttatt tctcaaccat
     1501 catagtatag caaaaactaa tctttttgtc aatgtttttc atacagtttg tcttcctttt
     1561 ctgaaaatga ttagattagc agactttcta gcagtgattc ccgcttcttt gctatactaa
     1621 agaaaaacga ggtgaaccca catgaaaaaa atcgaaccta ttccaccttc tcttcctctt
     1681 cttacaaagg gagagtttta tttagaagat gaggagatct tctctggtct tctgatcaac
     1741 gggggtgacc aaagctatct cgatgccaaa aatatggtat taagagagag tcatatccaa
     1801 aaacttacga tgcaaaaaac agttttacgt cattttgaat gcagtaatgt catttttgaa
     1861 aaatgtgatt tttccaatct ggagtggatt ggtggcagtt tccaccaagt cgtgtttcat
     1921 caatgtaaat taacaggtac gaattttgcc gaaagttatc ttcgagactg tactttcact
     1981 gattgtattg cttcgatggc ttcctttagt agtacaaact tgaaagctgt ctccttcgat
     2041 cattgtcaat tagaagacag cgaattttat gaggtcactt ggaaaaacct ttttttgtct
     2101 gacaatcagt tgaccggttc gaactggttt cgaacttctc tgaagggatt agactttacg
     2161 actaatttct tcacagcaat cgctttatct caagattctt tggcgggact aatcgtcaac
     2221 caagagcaag cacttgtgat cgcccaagcc cttggtttgc agatcaaaga ctagatcaac
     2281 gaatccgccg ccacagtgca ataagcatcc aaatagataa gaccaaactg atgatcaagg
     2341 cgattcccca tccgaaaacg gaatgggtaa agggtaactg gagattcatt ccgaagaatc
     2401 ctgtcacaat agttggaacg gtcaagatca gcgaccagac cgtcaaaaat ttcatcgtgt
     2461 cattcaagtt gttattcagc aaattattat aagtgccaga gagctggtct aaaatctgcg
     2521 aagctaagtt cgtcatttca actgcttgtc gtgcttcgat cagcgcatcg tccaactctt
     2581 ctttttcctt ttctgacaac ttgcgataaa tagccaatgc cttgatttgt tctaaaagta
     2641 ctacattttg ttttgttcct gtaactaagt aaaccaaacc gatctctaaa tctgaaagtt
     2701 gcagcagatt cttgttcgtt gttttttctc ttaatttcag attaagccgc tgacgttcac
     2761 tattcacttc ctcgattaaa ggaaaaaagg aatcagaaat caaaaacaat gtacgaaaca
     2821 ataaactgta tacactcatg tctggcattc gttccaaaag cgatcgaatt agtcgaatca
     2881 cgtactcggt attcggcgtg gtaaatgtaa acaaaccgtc ttcttttaaa ataaaagcga
     2941 taggactagt ttcataatga ttatcgactt tttgctggtg cggaacattt gatacaagca
     3001 gtaacgcttc atcaaaagaa tcgtactcta cgcgcgcccg ttcattttta tctagagaat
     3061 aagtcagcat ctcgtcacta atgccatatt cttcttttaa gtttttaaca gctagcacat
     3121 cgttttgctg tacattgatc caaaaattac ggtcttgatt aaaatagtgt ttttcccgca
     3181 tactctatcc tcctgacatt tgtttccagt ataaagaaaa aagtcgctca aataaaggtt
     3241 taccctttat ttgagcaact gtaaatcaaa tggctacttt ggtttggcga atctctgcta
     3301 atttttcttc gatcattttc aagacttctt cttttgcttg aggatcttcc acaaaattat
     3361 agcggtctcc atcgatttgg atttttggac tttctttgta ttcttcatac cattgatcgt
     3421 aacgtctgtt caattctttg taataatcgt atagacttgg atcaaaggac aattgttcat
     3481 acgaacgacc acgtttttcg atacgctcca acatcgtatc aaaagataca cgaatatgaa
     3541 ctaataaatc cggatgtttt ttatgggcag catagggaag ttcttccatc atgttttcca
     3601 gcagttcatc atacacacga acttctgttt ctgttgctct tcctagatct gcatttagat
     3661 ggaaaagcag tgaatcttca taaatcgaac ggtccaatac attattgtca tctttcattg
     3721 cttgtttgat gcttgcaaaa cgtttattca aaaaataaat ttgcagcaaa aaagcatact
     3781 gttcagggtt tgcataaaat aagggcaaga cttcattatc atcaatcgat tcataaaatg
     3841 cctgactatc aaagtgttca gccatcattt ctgttaaact tgattttccg gcaccaattg
     3901 tacctgctaa aacgatcacg ctcatttacc tccagctcac ttcatcacta aatattgtgt
     3961 ttaactccaa cctttttaaa tataccccca atatctagta gtgtcaattg tatttttttc
     4021 tagtcaaaga agaaaaaaag tcaaaaaatt cttaagaaaa aagtgttttt tctttagaaa
     4081 acgtattctt agttttactt ctgagatggt tttagtaaaa taaaggagga atgttttttg
     4141 agacccttag tggacaaaaa aattcgtgaa gcagcgcaac tcgttttggg cgctttattt
     4201 tttgcttaaa aatcactttt acgtgagaag gagttagatt atggcaaact atggccaagg
     4261 agaatcaagc ggaaagatca tattgatggg cgagcacgcg gttgtttatg gagaaccagc
     4321 gattgccttt cctttctatg caacaaaagt caccgcattc cttgaagagc tggatgcaat
     4381 ggacgatcaa ctggtttctt cctactattc aggaaattta gccgaagctc ctcatgcatt
     4441 aaaaaatatc aaaaaattat tcattcactt aaaaaaacag catgacatcc aaaaaaactt
     4501 gcaactgacc attgaaagca cgattcctgc tgaacgtgga atgggatcaa gcgctgcagt
     4561 cgccacagca gtcactcgtg ctttttatga ttacttagca tttcctttgt ctcgtgaaat
     4621 actattagaa aatgtccagc tttcggaaaa aatcgcccac ggtaatccta gtggaatcga
     4681 tgcagccgct actagcagct tgcagccgat ttattttaca aaagggcatc ctttcgacta
     4741 cttttctttg aacatcgatg cttttttgat tgtcgctgat acaggaatca aaggacaaac
     4801 aagagaagcc gtcaaagatg tcgctcacct ctttgaaacc cagcctcatg aaactggaca
     4861 aatgatccaa aaattaggat acttgacgaa gcaagcaaaa caagcaatca ttgaaaattc
     4921 accagaaacg ttggcacaga caatggatga atcacaatca cttctggaaa agctgacaat
     4981 cagcaatgat tttcttaatc tattgatcca aacagcaaaa gataccgggg cattgggtgc
     5041 caaattaact ggcggtggac gcggtggctg tatgattgca ttagcacaaa caaaaacaaa
     5101 agcccaagaa atcagtcaag cacttgaaga tgcgggtgct gctgaaactt ggatacaagg
     5161 attaggagta catacctatg tttaaaggca aagcacgcgc atataccaac attgctctaa
     5221 tcaaatattg gggtaagaaa aatgaagaac tcatccttcc aatgaacaac agcctttcgt
     5281 taacattgga tgcattttat acagagacag aagtcatctt ttctgatagc tatatggtag
     5341 atgaatttta tctagatggc accttgcaag acgaaaaagc aacaaaaaaa gtcagtcagt
     5401 ttcttgacct ttttcgtaaa gaagccggcc tctcactaaa agcttcagta atcagtcaga
     5461 atttcgttcc taccgcagct ggattagcct cctctgccag cgggctagct gctttagcag
     5521 gcgcttgcaa tactgctctt aagcttggat tagatgatct ctctctttca agatttgctc
     5581 gacgcgggtc tggttcagct tgccgaagta ttttcggtgg tttcgtcgaa tgggaaaaag
     5641 gccatgacga cttaagttct tacgctaagc cagtcccttc cgattctttc gaagacgatt
     5701 tagcaatggt tttcgttttg atcaacgacc agaaaaaaga agtgtccagc agaaatggga
     5761 tgcgtcggac agtcgaaaca tccagttttt atcaaggctg gttagattcc gttgaagggg
     5821 atctatatca attgaaacaa gcaatcaaaa caaaagattt ccaacttctc ggagaaacga
     5881 tggaaagaaa cggactaaaa atgcatggaa cgactttagc tgcccagcct ccattcactt
     5941 actggtctcc tgacagctta aaagcgatgg atgcggtgcg acaattgaga aagcaaggca
     6001 ttccatgcta ctttacgatg gatgctggcc ctaatgtcaa agttttagtg gaaaacagcc
     6061 atttatctga ggtacaagaa acatttacca aattatttag caaagaacaa gtaattactg
     6121 cacatgctgg tccaggaatt gcgattattg aataaaggga gatttttatg attgaagtat
     6181 ctgcaccagg caaactctat attgccggag aatatgcagt tgttgaaaca ggccatccag
     6241 cagttatcgc tgcagtcgat caattcgtga cagtcactgt agaatctgca cgaaaagtcg
     6301 gaagtatcca atctgcacaa tatagtggga tgcctgtacg ttggacaaga cgcaacggag
     6361 aattagtttt agatattcgg gaaaatcctt ttcattatat ccttgctgct attcgcttga
     6421 ctgaaaagta tgcacaagaa aaaaacattc ttttatcctt ttatgatctg aaagtaacaa
     6481 gtgagttaga cagttcaaat ggccggaaat atggattggg ttctagcggt gccgtgaccg
     6541 ttgcaacggt caaagccttg aatgtttttt acgcgttgaa tttatctcag ttggagattt
     6601 tcaagattgc agcactagcc aatttagcag ttcaagataa tggttcctgc ggcgacatcg
     6661 ctgctagctg ttatggtggc tggatcgctt tctcaacctt cgaccatcct tggctccaag
     6721 aacaagaaac tcagcattct atcagtgagt tacttgccct ggattggcca ggtctatcca
     6781 ttgagccatt gattgctcct gaagatttac gtttattgat tggttggacg ggtagccctg
     6841 cctctacttc tgatttggtc gatcaagttc accgttcgag agaagataaa atggtggctt
     6901 atcagctttt cttaaaaaat agtacagaat gtgtcaacga aatgatcaaa gggtttaaag
     6961 aaaataatgt aacgttgatc caacagatga ttcgaaaaaa ccgacaatta ctgcatgatt
     7021 tatctgcaat cactggggtc gtcatcgaaa cgcctgcttt gaacaaattg tgtaatttag
     7081 ctgaacagta tgaaggagcc gcaaaatctt ctggtgcagg tgggggcgat tgcggaatcg
     7141 taattgttga ccagaaatct ggcattcttc ctttaatgag tgcatgggaa aaagcagaaa
     7201 tcactccact gccgttacat gtctatagcg atcaaagaaa ggaaaaccga tgaatcgaaa
     7261 agacgaacat gtttcactag ctaaagcctt tcatgataaa caaaaaaacg aatttgactt
     7321 cgtacggatc atccataacc ctttgccgca aatagcagtt tctgatgtcg atctaagtac
     7381 acaggcagtt gggtttacac taagcagccc attttatatc aatgcgatga ctggcggaag
     7441 cgaaaaaaca aaaaaaatca accaagatct tgcaatcgtc gcaagagaag cagatttgat
     7501 gattgccact ggttcagtca gcgctgcctt gaaagtccct tctttagccg atacttatac
     7561 gattatgcga caagaatatc ctcacgggaa aatcattgcc aacatcggtg cagggacttc
     7621 tgtcgaaaga gccaaagaag ctatccgatt gtttcacgca gatgccttac agattcattt
     7681 gaatgcacct caagagttgg ttatgcctga gggagaccgt gattttacca attggaaagt
     7741 acttattcaa gaaactcaaa cagccattga cgtccccctc atcgtaaaag aagtcggttt
     7801 tggcatgaca agagaaacgc tcaatgatct agctgcttta ggagttcata cagttgatat
     7861 cagcggtcga agcggaacaa gcttcaccca gatcgaaaac gcccgtcgct ccaagcggga
     7921 actgagttat ttagctgact gggggcaatc gacagtctct tcattgcttg aagcaaatga
     7981 agcagacact tctatggaaa tcctagcctc cggaggtatc cgcaatgcct atgacatatt
     8041 taaagccctt tgtctcggcg caaatgccgt gggtacttca ggaaccgtac tgacgcatct
     8101 gatgaatcac ggcgtagaag aaactattat actgatgaag caatggcaag aagagcttcg
     8161 tctgctttat actatggtcg gcgcaacgaa taccgcggcc ctacaccaac aatctctgat
     8221 cttttccgga ccagtgaagg attggtgcga agcaagaggg atcgacttag tgaagtatgg
     8281 aagacgcaca gaaaagagcg tgggacaaaa gtaaagaaca cttttgtctc acgctctaaa
     8341 cacgtacaaa cggcggaagt a
//
LOCUS       DACEEZ010000101         8293 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20313_2017
            SAMN12639966-rid9879223.denovo.101, whole genome shotgun sequence.
ACCESSION   DACEEZ010000101 DACEEZ010000000
VERSION     DACEEZ010000101.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639966
            Sequence Read Archive: SRR10023158
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8293)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8293)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741683.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741683.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:44:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,016
            CDSs (total)                      :: 2,962
            Genes (coding)                    :: 2,889
            CDSs (with protein)               :: 2,889
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 36 of 73
            Pseudo Genes (incomplete)         :: 29 of 73
            Pseudo Genes (internal stop)      :: 18 of 73
            Pseudo Genes (multiple problems)  :: 8 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8293
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639966-rid9879223.denovo.101"
                     /isolate="E20313_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(48..1514)
                     /locus_tag="IWO89_002479"
     CDS             complement(48..1514)
                     /locus_tag="IWO89_002479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide MFS transporter"
                     /protein_id="HAQ0457194.1"
                     /translation="MEKQQDKGFFGQPKGLSTLFFTEMWERFSYYGMRALLIYYMYDT
                     VANGGLGLPRATALAIMSIYGSLIYMSSIVGGWVSDRVLGAKRTVFFGGVFIMLGHIV
                     LALPFGVSTLFVSMALIIIGTAFLKPNVSGMVGHLYSKTDLRRDAGFSIFYMGVNLGA
                     LLAPLIVGTIGQEYNYHLGFSLAAIGMFFGLLQYIIQGRKTLDGVGTTPPNPLTTEER
                     KGFIRNLIIALVVIAAVFGGAQLTGHLTIDFFVNTISVLGIILPLYYFIKMLTSKDVT
                     AEEKPRVWAYIPLFLAAIIFWSLQEQGSSILALFASERTQSTLFGVTIQASWYQSLNP
                     IFIFILTPVFVTMWTKLGERQPSTVVKFSLGLLFAGLSYLLLMLPGMLYGTSGKVSPL
                     WLLGSFFIVVIAELCLSPVGLSVTTKLAPRAFESQTIAIWFLADASSQAINAQIARFY
                     TPGTESAYFGIIGAVAIVGGILLFTVKEPIKKLMGSIH"
     gene            1901..2497
                     /locus_tag="IWO89_002480"
     CDS             1901..2497
                     /locus_tag="IWO89_002480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HAQ0457195.1"
                     /translation="MTTFTDTLKNRRSIYHLGRNVSLSNEELTTLIKEAIKESPTAFN
                     AQSTRAVILFGDAHEKLWEITEEALRPLTPAEAFPNTQNKLAGFKNGYGTVLFFKDTD
                     VVKGLQEQFELYADNFPDWSEQSNGIATANTWVALVDKGLGANLQHYNPVIDEAVAKE
                     WNIPSNWKLRSQLVFGSPETPAGEKEYMNDADRFRVFG"
     gene            complement(2555..3037)
                     /locus_tag="IWO89_002481"
     CDS             complement(2555..3037)
                     /locus_tag="IWO89_002481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0457196.1"
                     /translation="MVTFFTVLLFLFVVVMFLFLWLVRKEIIYRTVRNRWVYLVIPFL
                     VSLVIWYTLISHPTADELAKGILSAMIFISFLLDSRGITEEGLVLNSFDKKGVPFSEI
                     NKIVLYQPKGSKIVKMNFFRNGWRGPMLKFSASLEELVPFLSQRLNDEAEIDIMIDPE
                     "
     gene            complement(3143..3340)
                     /locus_tag="IWO89_002482"
     CDS             complement(3143..3340)
                     /locus_tag="IWO89_002482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0457197.1"
                     /translation="MTEEMINLGEQYACKPIGFTKTVIGEVVSKMTNCAVVKVAQCAA
                     EDQELLDEKASMVVAKYDTFE"
     gene            3635..4393
                     /locus_tag="IWO89_002483"
     CDS             3635..4393
                     /locus_tag="IWO89_002483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase C39"
                     /protein_id="HAQ0457198.1"
                     /translation="MKKKTFKKLLFLGGTAALLLSGTSYYLHIHSPEHTMNTAQKSST
                     QKTKKTKEKAEVLLDVPLESQFDSPSLENGCEVTSLSMLLSFYGYETTKNQLAEQLDY
                     VPVFNADGTHGDPNEGFVGDISGGDWAMVVYVPPVASLAQQIVQTDYHTFPKTDAQLD
                     DIKKALSKGNPVWTSVTINFEVPDETDFMTWTTNNGEVRVTPLVHACVVTGYDKENIY
                     VNDPYGVKNRAVPINDFSAIFTAMGGQMLTLEKS"
     gene            4453..4881
                     /locus_tag="IWO89_002484"
     CDS             4453..4881
                     /locus_tag="IWO89_002484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0457199.1"
                     /translation="MEKFTHKKMDPNEIPIIFVRDCKGNVQGKVSINEWNERRRPATL
                     NELEIKLYRQSLVYYADQEYEKATDLLKFLIARTEYTRFEYIERLANIYHIMNEPVKE
                     YQLLDTVLSVAELIALPAGLEKKLVRRLLRVKQQLSDQEK"
     gene            4927..4999
                     /locus_tag="IWO89_002485"
     tRNA            4927..4999
                     /locus_tag="IWO89_002485"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4960..4962,aa:Gln,seq:ctg)
     gene            complement(5149..5520)
                     /locus_tag="IWO89_002486"
     CDS             complement(5149..5520)
                     /locus_tag="IWO89_002486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YxeA family protein"
                     /protein_id="HAQ0457200.1"
                     /translation="MKVIKYLIGLAAISGILLFGLKYYTEGSYGEIPGILDQLNPLVE
                     KGEVYVKTQKPEEVNEYGTARYVQKAADANGKERTVEFNGLSVLKENHYLKISNKGAH
                     IETYEEVPREKVPEKALAIIG"
     gene            5735..5920
                     /locus_tag="IWO89_002487"
     CDS             5735..5920
                     /locus_tag="IWO89_002487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0457201.1"
                     /translation="MNQEELFQKVKEMIKNGNFDGAKRFIEEHKEQLGPYKEKAQNLL
                     KDVNIDSVKNKFKNLFK"
     gene            complement(6003..6191)
                     /locus_tag="IWO89_002488"
     CDS             complement(6003..6191)
                     /locus_tag="IWO89_002488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0457202.1"
                     /translation="MNPYEQLIQDLIDGKIEKIDVKREELMAFREAWLKLENRKYVVG
                     KASLNGNVTYHYDPTRLF"
     gene            complement(6215..6478)
                     /locus_tag="IWO89_002489"
     CDS             complement(6215..6478)
                     /locus_tag="IWO89_002489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0457203.1"
                     /translation="MDHVVLEIDLHLINNIRVIYYVVSNSVEQRVLTNKINGILAKKD
                     VHRFNNGEGSYYSIPVEKIIYTTVKVREDLDTKKAYEPIFTTY"
     gene            6636..6893
                     /locus_tag="IWO89_002490"
     CDS             6636..6893
                     /locus_tag="IWO89_002490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose uptake protein"
                     /protein_id="HAQ0457204.1"
                     /translation="MIAIGQFVFYIPFFIMISILFYYIKWTKKKFSVLLASLPAVYFT
                     YQIFSFRHWETTSVLITHIIELTLSVIFLIIWIYFLYKNQN"
     gene            complement(6964..7155)
                     /locus_tag="IWO89_002491"
     CDS             complement(6964..7155)
                     /locus_tag="IWO89_002491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0457205.1"
                     /translation="MEAGKVLQEVKALCADKKYEEAKSLIEKNKENLEEKYPIAQEFI
                     ELKQGTILHRFKHFFGITE"
     gene            complement(7296..7616)
                     /locus_tag="IWO89_002492"
     CDS             complement(7296..7616)
                     /locus_tag="IWO89_002492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0457206.1"
                     /translation="MVRPQEVRAPKEKIEILAIIEDGTQTKKGYSIALVKWKGKKGVA
                     LRWDGDNQQDKGFPVTANGYHPAWFVLPDKFMELYSYDYARAAASIKALDKLTEEQNE
                     MEEK"
     gene            complement(7639..8073)
                     /locus_tag="IWO89_002493"
     CDS             complement(7639..8073)
                     /locus_tag="IWO89_002493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HAQ0457207.1"
                     /translation="MSKRYQNIMVAVDGSRQSIKAFAEALDLAKDNEAHLFIVSIINK
                     VELTHSAYAFSKIYADEKQRTEVAMLKKINDAKEYGIAEIHAIVETGDPRNLIANVIP
                     GQENIDLIVMGATGKGAIQQALVGSTASYVVSHAPCSVLVVK"
BASE COUNT         2630 a         1636 c         1314 g         2713 t
ORIGIN      
        1 aacaggaact gctgcaaata agcaattcct gtttttatgt tcattgatta atgaatagag
       61 cccattagtt ttttgattgg ttctttcaca gtaaatagca agataccacc gacgattgct
      121 actgcaccga taataccgaa gtaagcagat tctgttcccg gtgtgtagaa gcgtgcaatt
      181 tgtgcattga ttgcttgtga agaagcatca gctaagaacc aaatagcgat tgtttgtgat
      241 tcaaatgctc gtggtgctaa ttttgttgtc acagacaaac caactggtga aagacacaat
      301 tcagcaataa ccacgatgaa gaaacttccc aacaaccaca atggacttac ttttccactt
      361 gtaccataaa gcatacccgg caacatcaat agcaagtacg ataatcctgc aaataaaagt
      421 ccaagagaga atttcactac tgtagatggt tgacgttcac caagtttcgt ccacatcgtt
      481 acgaatactg gtgtcagaat aaagatgaaa attggattca atgattgata ccagcttgct
      541 tggatcgtta caccaaatag cgtagattgt gtacgttcac tagcaaataa agctaagata
      601 gatgaacctt gttcttgtaa tgaccagaaa atgattgctg caaggaataa tgggatataa
      661 gcccatactc ttggtttttc ttcagcagtt acatcttttg aagtcagcat tttgataaag
      721 taatacaacg gtaagataat ccccaataca ctgattgtat tgacaaaaaa gtcaattgtc
      781 agatgaccag ttagttgtgc cccgccgaat actgcagcga taacaacaag tgcgatgatc
      841 aagttacgga tgaatccttt acgttcttca gttgtcaaag gattcggcgg tgttgtaccc
      901 actccgtcta atgtcttgcg tccttggatg atgtattgta ataatccaaa gaacatccca
      961 atagcagcta aagagaaccc taaatgatag ttatattctt gaccgattgt accaacgatt
     1021 agtggtgcta ataatgcccc taagttgaca cccatgtaaa aaatcgaaaa tcctgcatct
     1081 cttcgcaagt cagtctttga ataaagatgt ccgaccatgc cagaaacatt tggtttcaaa
     1141 aaggcggttc cgataataat caatgccatc gaaacaaaca atgtacttac accaaatggt
     1201 aaagccaaga cgatgtggcc aagcataatg aaaactccac cgaagaatac agtgcgtttt
     1261 gctcctaaca ctcggtcaga tacccatccg ccgacgatac tagacatgta gatcaaagat
     1321 ccgtagatcg acatgatagc taatgctgtt gctcgtggta atccaagtcc cccgttggca
     1381 actgtatcgt acatataata gataagtaat gcacgcatac cgtaataact aaatcgttcc
     1441 cacatctcag tgaagaacaa agttgacaat cccttcggct gcccgaagaa gcccttgtct
     1501 tgctgttttt ccataatcta taccccaatt ctttaaattt tcagaaaata ttatacatct
     1561 ttaccgaaaa atttaactaa aaaacttatt taagaaatat aaactaattt tctataaaag
     1621 caagtaattt ttaatggcaa tatcataaaa aaaagaagat agagatttaa taattatata
     1681 tcagctgttt ttttcaaata agcaaaaatt cctaaaacac aacaatgaaa gcgataacta
     1741 agaaaatcct tacataacga tttttcttat taaaattgtt ttttgaactt cgcttatttt
     1801 ttgtgagcat atctgtttac ttactttttg taaggtgcta tacttcattt cgtaatcaat
     1861 taaaaaaatg caaaacgcaa atcaaaggag aaattttatt atgacaactt ttactgacac
     1921 tttgaaaaac cgccgttcaa tctatcatct aggacgtaat gtttcacttt ctaatgaaga
     1981 attaacaact ttgatcaaag aagcaattaa agaaagccca actgctttca atgctcaatc
     2041 aacacgcgca gttatccttt tcggagatgc acatgaaaaa ctttgggaaa tcactgaaga
     2101 agcacttcgc ccattgactc cagcagaagc tttcccaaat acacaaaaca aattagctgg
     2161 tttcaaaaat ggttatggta cagtattatt cttcaaagat actgacgtgg taaaaggtct
     2221 acaagaacaa tttgaattat atgcagacaa cttcccagat tggtcagaac aatcaaacgg
     2281 aatcgcaaca gctaatactt gggtagcatt agttgataaa ggattgggcg caaacttgca
     2341 acattacaac ccagttattg acgaagcagt tgcaaaagaa tggaatatcc cttcaaactg
     2401 gaaactacgt tcgcaattag tattcggttc accagaaaca ccggctgggg aaaaagaata
     2461 catgaacgat gccgatcgct tccgcgtatt tggataattt tatttgaaat aaaaaacaga
     2521 ggttgtgaca aacatcttgt cgcaacctct gtttttattc tggatcaatc ataatatcaa
     2581 tctctgcctc atcgtttaag cgttgggaca aaaatggaac taattcttct aatgatgcag
     2641 aaaatttcag catcggtcct cgccatccgt ttcggaaaaa attcattttt acgattttag
     2701 aaccttttgg ctgatataat acaattttat tgatctcaga aaaaggaaca ccttttttgt
     2761 caaagctatt caaaacaagt ccttcttctg tgattcctct actgtccaac aaaaaactaa
     2821 taaaaatcat tgctgacaag atcccttttg ccaattcatc tgctgtagga tgactgatga
     2881 gtgtatacca aatgactagt gacactaaga agggtatcac caagtagacc catcgatttc
     2941 gcacagtccg ataaataatt tcttttcgaa ccagccaaag aaataaaaac atcacaacaa
     3001 cgaataaaaa caatagaacc gtaaagaatg ttaccatgac aactcgcctc gctttttctt
     3061 ctccagaaaa taggaaaaga atactgattt accttctcaa agaaattgga cttgcagcac
     3121 gccgcacgtc caattgtttt ctttattcaa atgtatcata ttttgcaaca accatgcttg
     3181 ctttttcatc taataattct tggtcctctg cagcacattg ggcaactttg accacggcgc
     3241 agtttgtcat ttttgataca acttcaccaa tgaccgtttt tgtaaaacca attggtttgc
     3301 atgcatattg ttctcctaaa ttaatcattt cttccgtcat caaaatcctc ctaaactgct
     3361 ggaacttttc tccacattac cactgctatt tctttggaga tttaactcat tgatttttat
     3421 ttacagttaa aaatcaacct tacaggcatt atagcgtact gttttcgttt tcacaattaa
     3481 aacagctgtt gtatttagaa gaattttttt caaaaaatta aaacatattc cttaaaaact
     3541 aattatttcc tacatattaa aatataatac ttccatttcc aataaaaata catttataat
     3601 aggaaagata aacagctgaa aagggagatt tcaaatgaag aaaaaaacgt tcaaaaaact
     3661 attgtttcta gggggcacag ctgctctttt attaagcggt acaagttatt acctacatat
     3721 ccattctcca gaacatacaa tgaacactgc acaaaaaagt tcaacacaaa aaacgaaaaa
     3781 gacaaaagag aaagcagaag ttttactaga tgttccatta gagagccagt ttgattctcc
     3841 ttctttagaa aatggctgtg aagtcacttc gctttctatg cttttatctt tttatggtta
     3901 tgaaacaact aaaaaccagt tagctgagca attggattac gtccctgttt tcaatgcaga
     3961 tgggactcat ggcgatccta atgaaggatt tgttggtgat atcagtggcg gagactgggc
     4021 tatggtcgtc tatgttcccc cagttgcctc ccttgctcag caaatcgtcc aaacggatta
     4081 tcacactttt ccaaaaaccg atgctcaact cgatgatatc aaaaaagcac tatctaaagg
     4141 aaatcctgtc tggacttctg tgactatcaa ttttgaagtc ccagatgaaa ctgattttat
     4201 gacttggacc acaaataatg gagaagtaag agtcactcct cttgttcatg cttgtgtagt
     4261 cactggatat gacaaagaaa atatttatgt caacgatccc tatggtgtaa aaaatcgagc
     4321 agtaccgatc aacgattttt cggcgatttt cactgcaatg ggtggacaga tgctaacttt
     4381 ggagaaaagc tagtaattca aacaaaaatc ccttatactc aagataccaa acctttgaag
     4441 ggagcgtaga taatggaaaa attcacacat aaaaaaatgg atccaaatga aattccgatt
     4501 atttttgtcc gtgactgcaa gggaaatgtt caaggtaaag tctctattaa tgagtggaac
     4561 gagcgtcgca gacctgctac cctcaatgaa ctggaaatca aattgtatcg tcaatctctt
     4621 gtttattacg cagatcaaga gtacgagaaa gccaccgatt tattgaaatt tctaattgct
     4681 cgaacagagt atactcgctt tgaatatatt gaacgtttag caaacatcta tcatattatg
     4741 aatgaacctg tcaaagaata tcagttattg gacaccgtct tgtcagtagc agaactgatt
     4801 gcccttcctg ctggattaga aaagaaacta gtacgccgcc ttttacgagt aaagcaacaa
     4861 ctatccgatc aagaaaaata acttgcgttt ttttaggaaa gtgctatgat tattttgttg
     4921 aataaatggg atgtagccaa actggtaagg cacttgactc tgaatcaagc aattgtaggt
     4981 tcgaaccctg ccatcccaat ttgatgtgct tgctgaaaaa atgaaccccc tgtaaaagaa
     5041 caatcttttt gcagggggat tattgtatta tttttactac ttgtatttag tttacataat
     5101 taaattatga ataataaaat cgggcgtatt cttaatacaa aaactgattt acccaataat
     5161 tgctaatgct ttctccggaa ctttttctct aggaacttcc tcatacgttt ctatgtgtgc
     5221 tcctttattg ctaattttca aataatgatt ttcttttaac acagaaagac cattaaattc
     5281 aaccgttcgt tctttcccat tggcatcagc agctttctgc acataccttg ctgtaccata
     5341 ttcatttact tcctctggtt tttgtgtctt tacatagacc tctccttttt caactaatgg
     5401 atttagctga tcaagtatac cagggatttc tccataactt ccttcagtat aatatttcag
     5461 tccaaatagc aaaataccac taatcgccgc cagtccaatc aaatatttaa tcactttcat
     5521 ctttctacct ccttgttata tattcagtat atgggtcagt taaagaaatt ccatccgtcc
     5581 caagttggag atttttctcc tactttagaa gcgagaatcg agtgtttttt tagtaaaaat
     5641 gatcgttatt tgatattatt gacgtgtgga taaatcagtt tttctcgttt tgtccatgta
     5701 aaaaatcatg ttgatagcaa aggatgtgtt caaaatgaat caagaagaat tatttcaaaa
     5761 agtcaaggaa atgattaaga acggcaattt cgatggcgca aaacgattta tcgaagaaca
     5821 taaagaacaa ttaggacctt ataaagagaa agcccaaaat cttttaaaag atgtaaatat
     5881 cgacagtgtc aaaaacaaat ttaaaaatct attcaaataa gtaaaacttt cacttttgta
     5941 aaatcaaagg aggacaatgc tgctgcatcg tcctcctttg gttcttttct gaatacttca
     6001 tactaaaata aacgtgtcgg atcgtaatga taagtcacat tgccgttcag tgaggctttt
     6061 cccaccacat atttacgatt ctccaacttc agccacgctt ctcgaaaagc cattaattct
     6121 tctcttttta catcaatctt ttcgattttt ccatcgatca aatcttgaat aagttgttca
     6181 tatggattca tttttttcct ccatctgctg tcaatcaata agttgtaaaa ataggttcat
     6241 atgctttttt ggtatctaga tcttctctga ctttgactgt cgtataaata attttttcta
     6301 ctgggatact ataataactt ccttcaccat tattgaatcg gtgaacgtct ttctttgcta
     6361 aaataccatt tattttattc gtcagtacac gttgttcgac actgttactt acaacatagt
     6421 aaatcacccg aatattattg atcaaatgca aatcgatttc taacactaca tgatccatga
     6481 aaatcccact ccttctttca ctttgattat agcaagtttc tcctctaatt tcaaaatcga
     6541 tacagatttt accggataaa tccacaaaac tgcttgcgcc atttcgcaca aagtgttatt
     6601 ttgtatcatg gactttattt atcggaggca atagtatgat cgcgatcggt caatttgttt
     6661 tttatattcc tttttttatt atgataagca ttcttttcta ttacataaaa tggacaaaaa
     6721 agaagttttc agttttactg gcctctttac ctgctgtata ttttacttat caaatttttt
     6781 cttttaggca ttgggaaaca acgtctgtat taataacaca catcatcgaa ttgacgcttt
     6841 cagtaatttt tcttattatt tggatctatt ttttatataa gaatcagaat tgaatccctc
     6901 gcatgtttaa aaagaaaacg attaaagatg cagcaaattt ctgccgatct ttaatcgttt
     6961 tttctattct gttataccaa aaaaatgttt gaatcggtga aggatcgttc cttgttttaa
     7021 ttctataaat tcttgtgcga tcgggtactt ttcttccaaa ttttctttgt tcttttcgat
     7081 caggcttttc gcttcttcat attttttatc tgcacatagt gcttttactt cctgtaatac
     7141 ttttcctgct tccatttgcc tcaccctctt gccattttat tgctttaagt tttatttaag
     7201 ttatacgcat tattctagtt tcatcccaaa taaccttttt tcatttttac tcctcgaact
     7261 aatcagtttt ctaaaggctg attggttcgt cttttttatt tttcctccat ttcattctgc
     7321 tcttctgtta atttatctaa tgctttgatt gatgctgcgg ctcttgcata atcatagctg
     7381 tataattcca taaatttatc aggcagaaca aaccacgctg gatgataacc gttagctgtg
     7441 acagggaatc ctttatcttg ttggttatct ccatcccatc tcagagcaac accttttttt
     7501 cctttccact ttactaaagc gatcgagtat cccttctttg tttgtgtgcc gtcttcaata
     7561 attgctaaaa tttctatttt ttcttttggc gcacggactt cttgaggacg aaccattttc
     7621 tattcctccc ttatctcatt acttcacgac caacacactg caaggggcat gtgatacgac
     7681 ataagaagcg gtcgatccaa ctaatgcttg ttgtatagca ccttttcctg tcgctcccat
     7741 aacgatcaaa tcaatatttt cttgtccagg gatgacattg gcaatcaaat ttcttggatc
     7801 tccagtttca actattgcgt ggatctcagc aatgccgtac tctttggcat cattgatttt
     7861 tttcagcatg gctacttctg ttctttgttt ttcatctgca tagatttttg aaaaggcata
     7921 ggcactatgg gtcagctcaa ctttgttgat gattgacacg ataaataagt gggcttcatt
     7981 gtcctttgct aagtcaaggg cttcagcgaa cgctttgatc gattgtcttg atccgtcaac
     8041 tgctaccatg atgttctggt atcttttact cattggaacc gcctcctttt tcttttattg
     8101 taacatctca agcaaaaata caaaaaatat acgcaatatg taaataaaat gatgaacaaa
     8161 agcaatttct ttcaagttat ttatacattt cttttctcat cagaacacct gttctaatcc
     8221 cattatttct gcattatgtg aaattcctca tcatttttca catcgagcga acatcattct
     8281 gttacaaaaa acg
//
LOCUS       DACEEZ010000102         8164 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20313_2017
            SAMN12639966-rid9879223.denovo.102, whole genome shotgun sequence.
ACCESSION   DACEEZ010000102 DACEEZ010000000
VERSION     DACEEZ010000102.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639966
            Sequence Read Archive: SRR10023158
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8164)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8164)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741683.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741683.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:44:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,016
            CDSs (total)                      :: 2,962
            Genes (coding)                    :: 2,889
            CDSs (with protein)               :: 2,889
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 36 of 73
            Pseudo Genes (incomplete)         :: 29 of 73
            Pseudo Genes (internal stop)      :: 18 of 73
            Pseudo Genes (multiple problems)  :: 8 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8164
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639966-rid9879223.denovo.102"
                     /isolate="E20313_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(571..1917)
                     /gene="gor"
                     /locus_tag="IWO89_002494"
     CDS             complement(571..1917)
                     /gene="gor"
                     /locus_tag="IWO89_002494"
                     /EC_number="1.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-disulfide reductase"
                     /protein_id="HAQ0457208.1"
                     /translation="MQIYDYIVIGGGSGGIASANRAGMHGAKVLLIEANELGGTCVNV
                     GCVPKKVMWQASTILETIERDASSYGIQADLKNVDFAGLVERREKYIDFLHGAYQRGL
                     DSNHVEAIKGYATFIDSQTIEVNGETYRAPKILIATGGRASKMTIPGGEYAIDSNGFF
                     ELTELPKRAIVLGAGYIAAELSGVFRGLGSEVMWAYRKERPLRTFDKMLSDNLIQMYQ
                     EAGIKTYAHHIAKEITKNNDEYTVLFENGETLTGDCVLFAGGRVPNTEKLGLENTNVE
                     LDKKGFIKVDKYQNTTDEHIFAVGDVIGKLDLTPVAIAAGRRLSERLFNGKKDSYLDY
                     KLVPTVVFTHPPIATIGLTEEEALEKYGENELKVYRSRFTPMYFALNDYRQKCEMKLI
                     CVGKEERIVGLHAIGVGVDEMLQGFAVAIKMGATKEDFDNTVAIHPTGAEEFVTMR"
     gene            complement(2051..3292)
                     /locus_tag="IWO89_002495"
     CDS             complement(2051..3292)
                     /locus_tag="IWO89_002495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HAQ0457209.1"
                     /translation="MTVKRKLKRPEILAPAGTLEKLKTAIHYGADAVYIGGNAYGLRS
                     RAGNFTYEEMAEGVAFAKEHDAKVYVAANMVTHEGNEDGAGEFFRSLRDVGISAVIVS
                     DPALIEICATEAPGLPIHLSTQASATNFETLEFWKEEGLERVVLAREVSMEEVAEIRK
                     NTDTEIEAFIHGAMCISYSGRCTLSNHMSMRDANRGGCSQSCRWKYGLFDMPFGQERN
                     SLTDTGEVEEEFSMSAVDMSMIEHIPELIENGVDSFKIEGRMKSIHYVSTVSNVYKAA
                     VDSYMEDPENYVCKQEWIDELWKVAQRELATGFYYNTPTENEQLFGERRKIPVYKFVG
                     EVLSYDEATQIATIRQRNLFSVGDEIEFYGPGFSHFSQTVEVMHNEEGESIDRAPNPM
                     MILTMPVAQPVKPGDMIRKRK"
     gene            complement(3310..4233)
                     /locus_tag="IWO89_002496"
     CDS             complement(3310..4233)
                     /locus_tag="IWO89_002496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HAQ0457210.1"
                     /translation="MIELITTVETIEQAEQLLAAGADTLYFGEEQFGLRLPASFTREE
                     QRQLVELAHKYGKKANIAVNGIIHPEKMKNVPEYLAFLKEINVDVITVGDTGIIYTMK
                     KNPELRIPYIYDAETLVTSARQINFWAKKGAVGAVLAREVPFEEMKAMEEKLDIPVET
                     LVYGATCIHQSKRPLLQNYYNYTKQDEQKDRERGLFISEPKKEETHYSIYEDSHGTHI
                     FASNDLNLSNELNELYQHHYCTWKLDGLFTPGEKFVNITRLFAEAKKQILEGSWDQEK
                     AEKMTSEISKLHPENRELDTGFFYIDPSAIK"
     gene            complement(4454..6946)
                     /locus_tag="IWO89_002497"
     CDS             complement(4454..6946)
                     /locus_tag="IWO89_002497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit"
                     /protein_id="HAQ0457211.1"
                     /translation="MEELFTERAQAVLEIAQEEAKRLKHQSVSSEHVLLALVVEQNGI
                     AGKVLREMDLNETEIYEEIEHLIGYGGIRSYPKGVFLPYSPRMKQVFALASSEVKKTG
                     SQKVGTEHILMSLLKDESIMATRIMINLGISLSKARQVLKQKMGLAGDSAGKGMNRRR
                     NVNVQDKRQTPQGTPTLDSLARDLTKLAKENKLDPVVGRSREVKRLIQILSRRTKNNP
                     VLVGEPGVGKTAIAEGLAQKIILGEVPEDMQEKRLMMLDMGSLVAGTKYRGEFEDRMK
                     KVIDEIYNDGQVILFIDELHTLIGAGGAEGAIDASNILKPALARGELQTIGATTLDEY
                     QKYIEKDSALERRFARIQVDEPTPEEAEVILQGLRTRYEEHHGVEITDEAVRAAVNLS
                     VRYITSRQLPDKAIDLIDESAAKVRLDQTDHLTKSTVIKLEIDELVQEKEAAIQKQDF
                     ENAAQLRRQEKALRKKLQKVSAIEAKQEQGYSDRVTEEDVATVVSEWTGVPLQQLEKK
                     ESERLLELEGLLHERVVGQDEAVKAVSRAIRRARSGLKDPDRPIGSFMFLGPTGVGKT
                     ELAKALSEVMFGSEDALIRVDMSEFMEKYSTSRLIGSPPGYVGYDEGGQLTEKIRQKP
                     YSVILLDEVEKAHPDVFNLLLQVLDDGHLTDSKGRKVDFRNTIMIMTSNIGATQIREE
                     KNVGFNVQDVTKDHKAMQKRILEELKKAFRPEFLNRIDETVVFHSLSKDEIHTIVQIM
                     SKAIIKRLKEQDIQVKITPSAIDVIGKAGFDPEYGARPIRRALQKEIEDRLSEALLGG
                     EIRLGDHVTVGASKGKITLNVRGPKKETVGSL"
     gene            complement(6953..7420)
                     /locus_tag="IWO89_002498"
     CDS             complement(6953..7420)
                     /locus_tag="IWO89_002498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CtsR family transcriptional regulator"
                     /protein_id="HAQ0457212.1"
                     /translation="MAHQNTSDLIESYIKKILENSEMIEIRRIEMADLFNCVPSQINY
                     VINTRFTIQRGYLVESKRGGGGYIRIAKVRISDKKQLLEQINQLFDDTISEKNAFAII
                     QKLYEDQIITKKEGNLMLSAIAKNTLNFNEYEDHTRARILRAFLERLSYEDGK"
     gene            complement(7556..7629)
                     /locus_tag="IWO89_002499"
     tRNA            complement(7556..7629)
                     /locus_tag="IWO89_002499"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(7593..7595),aa:Arg,seq:acg)
     gene            complement(7640..7725)
                     /locus_tag="IWO89_002500"
     tRNA            complement(7640..7725)
                     /locus_tag="IWO89_002500"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(7689..7691),aa:Leu,seq:taa)
     gene            complement(7742..7813)
                     /locus_tag="IWO89_002501"
     tRNA            complement(7742..7813)
                     /locus_tag="IWO89_002501"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(7779..7781),aa:Gly,seq:gcc)
     gene            complement(7825..7897)
                     /locus_tag="IWO89_002502"
     tRNA            complement(7825..7897)
                     /locus_tag="IWO89_002502"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(7862..7864),aa:Thr,seq:tgt)
     gene            complement(7915..7996)
                     /locus_tag="IWO89_002503"
     tRNA            complement(7915..7996)
                     /locus_tag="IWO89_002503"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(7960..7962),aa:Leu,seq:tag)
     gene            complement(8004..8076)
                     /locus_tag="IWO89_002504"
     tRNA            complement(8004..8076)
                     /locus_tag="IWO89_002504"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(8041..8043),aa:Lys,seq:ttt)
     gene            complement(8080..8152)
                     /locus_tag="IWO89_002505"
     tRNA            complement(8080..8152)
                     /locus_tag="IWO89_002505"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(8117..8119),aa:Val,seq:tac)
BASE COUNT         2236 a         1816 c         1359 g         2753 t
ORIGIN      
        1 ccgtttatca gaggttgtaa aatcagtgtt ttgtcctgag tattagagaa acaaatggaa
       61 aaatagttcc tcatattttg ccgttttttg agctaatacc gaaggacagc tgattgaaca
      121 ctatttgttc ggaaaaagtg actaactatg acacaatttt tttcacagtc ccttttttca
      181 cttgatttga ttcgtaccag cgaacttgac gagaactttc ttggtttggt aactatatgg
      241 tattctttta acatcctcgt ttgcttcttc taatttattt atacaaagtc acactttttt
      301 aaacatctgt tcttcgatac atttctattt cttttaaaca tcgataccaa tattttgaac
      361 ttaagagtgc ataccaaatg gattcattta ctcttcgtca gttacgaaga taatcaaaat
      421 ctccaacttc cctacttatt tcgttttgag aattatcgaa gcaatcttac ataaataact
      481 aaaaaacaaa aaaccagaaa atcagtatac tgattttctg gttttacatg gtttgttcaa
      541 taaaaagctg ttggaactgt taatattaaa ttatcgcatc gttacgaatt cttccgctcc
      601 tgttggatgg atggctactg tattgtcaaa gtcttcttta gttgcaccca tcttgatagc
      661 cacggcaaaa ccttgcagca tctcatcgac accaacacca atagcatgta agccgacgat
      721 tctttcttct tttccgacac agatcaattt catttcgcat ttttggcgat aatcatttaa
      781 tgcaaagtac attggggtaa agcgtgaacg ataaactttc aattcatttt cgccatattt
      841 ttccaatgct tcttcttctg tcaaaccaat tgttgcaatt ggtggatgcg taaagacgac
      901 tgtcggaaca agtttatagt caagataaga atctttcttt ccattaaata aacgttctga
      961 taaacgtcta ccagctgcga ttgcaacagg agtcagatct aatttaccga tcacatcacc
     1021 aacagcaaaa atatgttcat ccgttgtatt ttggtattta tctactttga taaacccttt
     1081 tttgtccaat tccacatttg tattttccag acctaatttt tcggtattcg gtacacgtcc
     1141 accggcaaaa agtacacaat caccagttaa ggtttcacca ttttcaaata gcaccgtata
     1201 ttcgtcattg tttttagtaa tttctttagc aatatgatgt gcgtaagttt ttattccagc
     1261 ttcttggtac atttggatca aattgtccga aagcatttta tcaaacgttc taagtggtcg
     1321 ttcttttcga tatgcccaca tgacttcact acctaaacca cgaaatacac ctgaaagttc
     1381 agccgcaatg tatccagcac ctaaaacgat tgctctctta ggtaactctg ttaattcaaa
     1441 aaatccattt gaatcaattg cgtattctcc accaggaatg gtcattttac ttgcacggcc
     1501 tccagtagcg atcaagattt taggagcacg ataggtttca ccgtttactt cgatcgtttg
     1561 actatcgata aaagtagcgt aaccttttat agcctcgaca tgattgctat ctaatcctct
     1621 ttgataagca ccgtgtagaa aatcaatata tttttctcgt ctttctacta gaccggcgaa
     1681 atcaacattt tttaaatctg cttggatacc gtaacttgag gcatctcgtt caattgtttc
     1741 caaaatcgta cttgcttgcc acataacttt tttaggtaca caaccgacgt tgacacatgt
     1801 tccgcccaat tcattcgctt caatcagcaa aacttttgcg ccatgcatcc ctgctctgtt
     1861 tgctgaagca ataccgccag agccgccacc aattacgatg taatcatata tttgcatttt
     1921 gttcctcctt attgaagaga tcaatcaaaa cctttattta acgatccttt ttctacagaa
     1981 aacaagtgta acacttttct tgaattttgt atgcgaagat gcttaaaaca gggatatatc
     2041 agctttttcc ttatttacgt tttcggatca tatctccagg tttcaccggc tgtgcgactg
     2101 gcatagttaa aatcatcatt ggatttggtg ctcggtcaat ggattcgcct tcttcattat
     2161 gcataacttc aacggtttga ctgaaatgac tgaatcctgg accataaaat tcgatttcat
     2221 ctcctacaga aaacaagttt ctctgacgta tcgtagcaat ctgtgttgcc tcatcataag
     2281 ataatacttc accgacaaat ttatatactg ggattttacg acgttcaccg aataattgtt
     2341 cgttttcagt tggtgtattg tagtaaaaac ctgtagctaa ttctcgttgt gctactttcc
     2401 ataactcgtc gatccattct tgcttgcaga catagttttc aggatcttcc atataactgt
     2461 ctacagccgc tttatagaca tttgacaccg tagatacata atggatcgat ttcattcttc
     2521 cttcgatctt gaagctgtct acgccgtttt caatcaattc tggaatatgt tcgatcattg
     2581 acatatccac tgcactcatc gagaattctt cttccacttc gcctgtatca gttaaactgt
     2641 ttcgttcttg accaaaaggc atatcgaaca atccatactt ccagcgacaa gattgagaac
     2701 atccgccacg gtttgcatcc cgcatcgaca tatggttgga caatgtgcat cgaccagaat
     2761 aagagataca catggcacca tggataaatg cttcgatttc ggtatccgta tttttacgaa
     2821 tctctgccac ttcctccatt gaaacttcac gagctaaaac gacacgttcc aacccttctt
     2881 ctttccagaa ttccaatgtc tcaaaattag ttgctgaagc ttgagtggat aaatggatcg
     2941 gcagacctgg ggcttctgtc gcacaaattt cgatcaaagc tggatcagaa acaatcactg
     3001 cagaaatacc gacatcacgc aaactgcgga aaaattcccc tgctccatct tcatttcctt
     3061 catgggtgac catattagca gcaacgtata ccttcgcatc atgttctttt gcaaaagcaa
     3121 ctccttcagc catttcttca taagtaaaat tacctgcacg gcttcttaaa ccatatgcat
     3181 tcccgccaat atatactgca tctgctccat aatggatcgc ggtttttaac ttttccagtg
     3241 ttcctgctgg cgctaggatt tctggtcgtt tcaattttct ttttactgtc atttctttca
     3301 tcatactcct tatttgatag cagaaggatc aatataaaag aatcctgtat ctaattcacg
     3361 gttttcagga tgcaatttac tgatttcgct agtcattttt tccgcttttt cttgatccca
     3421 gctaccttct aaaatttgtt tttttgcttc ggcgaatagt cgagtgatgt ttacgaattt
     3481 ttcgcctgga gtaaataatc cgtccaattt ccatgtacag taatgatgct gataaagttc
     3541 attcaattca ttcgagagat tcagatcatt gctggcaaaa atatgtgtgc catgactatc
     3601 ttcataaata gaataatgtg tctcttcttt tttaggttcc gagataaaaa gacctctttc
     3661 acgatctttt tgttcgtctt gtttcgtata gttatagtaa ttctgtagca gtggacgttt
     3721 ggattgatgg atgcatgtag caccatacac aagggtttct acaggaatat cgagtttttc
     3781 ttccatcgcc ttcatctctt caaaaggaac ttcacgagca agtactgctc caactgcgcc
     3841 ttttttcgcc cagaaattga tttgacgtgc gctcgttact aatgtttcag catcataaat
     3901 atacggtata cgcaattcag gatttttttt catcgtataa ataatacctg tatcaccaac
     3961 tgtgatcaca tctacgttta tttcttttaa aaaagccaaa tattcaggta catttttcat
     4021 tttttcagga tgaatgatcc catttacagc aatattcgct tttttaccat acttgtgtgc
     4081 taattctacc aattgtcgct gttcttctcg tgtaaatgaa gcaggcagac gcaatccaaa
     4141 ttgctcttca ccgaaatata gtgtatctgc acctgcagca agcaactgtt ctgcttgttc
     4201 aatagtttct actgtggtta tgagttcgat cataattttc tcctcctaca acttcataat
     4261 agtagcatag ctctaaaaac tttccaatac ttcttctatt taaagtcgga tttttgaaaa
     4321 ttatttcaca aaaaatcaaa ataaagttcg atatattctg tccaaatata aaaagagatt
     4381 gtgacaaaaa tcttgtcaca acctcttaat caagtcttac tattataaaa attcagctac
     4441 acaggccaag taactatagg gaacctactg tttccttttt aggtccgcga acattcaatg
     4501 tgatcttacc ttttgaagca cctactgtta cgtggtcacc taaacgaatt tctccgccaa
     4561 gcaaagcctc gctcaatcga tcttcaattt ctttttgaag tgcgcgacgg attggacgtg
     4621 caccatattc aggatcaaat ccagctttcc cgatcacatc gattgcagat ggtgtgattt
     4681 tcacttgaat atcttgttct ttcaatcgtt tgataatcgc tttgctcatg atttggacga
     4741 ttgtatggat ttcatcttta ctcaatgaat ggaagacgac agtctcatcg attcgattca
     4801 agaattcagg tcggaaagct ttctttaact cttctaagat acgtttttgc atggctttgt
     4861 gatctttagt aacatcttgg acattgaatc cgacattttt ttcttctcga atttgtgtcg
     4921 ctccgatatt tgaagtcata atcatgattg tatttctaaa gtccactttt ctacctttag
     4981 aatctgttaa atgaccatca tccaatactt gcagaagtaa attgaaaacg tctggatgtg
     5041 ctttctccac ttcatctaag agaatcactg aatatggttt ttgacgaatt ttttctgtca
     5101 actgtccacc ttcatcatac cctacgtatc ctggaggaga cccgattaat cggcttgtac
     5161 tgtatttttc cataaattct gacatatcaa cacggatcag ggcatcttca cttccaaaca
     5221 tgacttctga aagtgctttg gccaattctg tttttccgac accagttggt ccaaggaaca
     5281 tgaatgaacc aattggacga tcaggatctt tcaatccgct tcgtgcacgg cggatcgcac
     5341 gtgaaactgc tttgacagcc tcatcttgac caacgacacg ttcatgcaga agtccttcca
     5401 gttctaacaa gcgttcgctt tcttttttct ccaactgctg tagagggaca ccagtccact
     5461 ccgatacaac tgtagcaaca tcttcttctg ttacacgatc agaataacct tgctcctgtt
     5521 ttgcttcaat tgcagaaact ttttgcagct ttttacgtag tgctttttct tgtctgcgta
     5581 attgagcagc attttcaaag tcttgttttt ggatcgctgc ttccttttct tgtactagtt
     5641 catcgatttc tagtttgatc acagttgatt ttgttaagtg atcggtttga tccaaacgga
     5701 ctttcgctgc tgattcatcg atcaaatcaa ttgctttatc gggtagttga cgagaagtga
     5761 tataacgaac agaaagattg actgctgctc gtacagcttc atctgtgatc tccactccat
     5821 ggtgctcttc atatcttgta cgtaatcctt gaagaatcac ttctgcttct tcaggagttg
     5881 gctcatctac ttgaatgcga gcaaaacgtc gctctaatgc agaatctttt tcgatatatt
     5941 tttgatattc atctagggtc gttgctccga tcgtttgtaa ttctcctcga gctaacgccg
     6001 gtttcaagat attcgaagca tcaattgctc cttctgctcc acctgcacca attaaggtat
     6061 gcaattcatc aatgaataag attacttgac catcattata gatttcgtcg atgacctttt
     6121 tcattcgatc ttcaaattca ccgcgatatt tcgtaccagc aaccaaagat cccatatcaa
     6181 gcatcatcag gcgcttttct tgcatgtctt ctggcacttc acctaaaatg attttttgag
     6241 ctaacccttc agcaatggct gttttgccta caccaggttc acccaccagt acagggttat
     6301 tctttgtccg tcggctaaga atctggataa gccgtttcac ttctctcgaa cgaccaacaa
     6361 ctgggtccag cttgttttct tttgctaatt tcgtcaaatc acgtgctaat gaatctagcg
     6421 taggtgttcc ttgcggtgtt tgccgtttat cttgtacatt tacatttcta cgacgattca
     6481 ttccttttcc agcagaatca ccagctagcc ccattttttg tttcagaact tgacgggctt
     6541 ttgaaaggct gatacctaag ttgatcatga ttcgagtagc catgatactt tcatctttta
     6601 ataagctcat caaaatatgt tctgttccaa ctttttgaga accagttttc ttcacttcgc
     6661 tgctagctaa agcaaaaact tgtttcattc ttggtgaata tggaagaaag actcctttag
     6721 ggtaagaacg gataccacca taaccaatca gatgttcaat ttcttcataa atttccgttt
     6781 cattcaaatc catttcacgt aatacttttc cagcaattcc attttgctcg accactaagg
     6841 ctagtaaaac atgttctgaa ctaactgatt gatgtttcaa ccgttttgct tcttcttgtg
     6901 caatttctaa tactgcttgg gctctttcag taaaaagttc ttccatctaa tctcacttcc
     6961 catcttcata acttaaacgt tccagaaatg ctcggagaat ccgagcacgt gtatgatctt
     7021 catattcatt gaaattcaat gtgttttttg caattgcact caacatcaag tttccttctt
     7081 tttttgtaat tatttggtct tcataaagtt tttgaataat agcaaaagca tttttctcac
     7141 tgattgtatc atcaaataac tgatttatct gctcaagtaa ttgtttttta tctgaaatcc
     7201 ttacttttgc aattcgtata taaccaccac caccacgttt gctttctact agataaccac
     7261 gttggatggt aaaacgggta ttaatcacat agttgatttg tgaaggaaca cagttaaaga
     7321 ggtctgccat ttcaatcctt cgaatctcga tcatttcact attttccaga attttcttta
     7381 tatatgattc aattaaatcc gaggtattct gatgtgccat cttattcatt ccctttgact
     7441 aatattgacc ttaattataa cgaatttgat aatagaaaga aagaagaacg ctttttgtta
     7501 aataaatcct ttagaatcaa caaaaaaaaa tacggaatat actattccgt atcgacgcgc
     7561 ccaagaggag tcgaacccct aaccttttga tccgtagtca aacactctat ccagttgagc
     7621 tatgggcgca tatttgaaat gccgaggacc ggaatcgaac cggtacggtg atcactcacc
     7681 gcaggatttt aagtcctgtg cgtctgccag ttccgccacc ccggcgcgat tgctttggca
     7741 aagcggaaaa cggggttcga acccgcgacc cccaccttgg caaggtggtg ctctaccact
     7801 gagctatttc cgcgtgttta aagatgccgg ctaaaggact tgaaccctcg accctctgat
     7861 tacaaatcag atgctctacc aactgagcta agccggcatg taaaacttaa attatgcggg
     7921 tgaagggact tgaaccccca cgccgtaagg cgctagatcc taaatctagt gcgtctgcca
     7981 attccgccac acccgcaaaa atatgagccg tacagggctc gaacctgtga ccctctgatt
     8041 aaaagtcaga tgctctacca actgagctaa cggctcatat ggaggttaac gggatcgaac
     8101 cgctgaccct ctgcttgtaa ggcagatgct ctcccagctg agctaaacct ccatggataa
     8161 ctta
//
LOCUS       DACEEZ010000103         8112 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20313_2017
            SAMN12639966-rid9879223.denovo.103, whole genome shotgun sequence.
ACCESSION   DACEEZ010000103 DACEEZ010000000
VERSION     DACEEZ010000103.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639966
            Sequence Read Archive: SRR10023158
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8112)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8112)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741683.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741683.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:44:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,016
            CDSs (total)                      :: 2,962
            Genes (coding)                    :: 2,889
            CDSs (with protein)               :: 2,889
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 36 of 73
            Pseudo Genes (incomplete)         :: 29 of 73
            Pseudo Genes (internal stop)      :: 18 of 73
            Pseudo Genes (multiple problems)  :: 8 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8112
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639966-rid9879223.denovo.103"
                     /isolate="E20313_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(<1..110)
                     /locus_tag="IWO89_002506"
     CDS             complement(<1..110)
                     /locus_tag="IWO89_002506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HAQ0457213.1"
                     /translation="MSKRTRRTFSQEFKQQIVNLYLAGKPRVEIIREYELT"
     gene            complement(179..385)
                     /locus_tag="IWO89_002507"
     CDS             complement(179..385)
                     /locus_tag="IWO89_002507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0457214.1"
                     /translation="MKKMGKCILCEKYTELTKEHVPPKKSGNTGGKKTRTGNLDDFLK
                     SDFTKGDFPKGIKRKPQGNVYYTG"
     gene            complement(548..2182)
                     /locus_tag="IWO89_002508"
     CDS             complement(548..2182)
                     /locus_tag="IWO89_002508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HAQ0457215.1"
                     /translation="MEMIQTKRVALYIRVSTQEQALEGHSIQAQEQNGRQFAERMGYE
                     VVEVYADEGISGKSTKNRFAYQRMMNDARNHRFDLLVIWKLTRLGRNMLDVLQTVEEL
                     LRYDVGLHSISEQFDVTTSTGKLMLQLLGSFGEFERNQIAENVQMSMQSLVKDKKRYA
                     GGRRLGYVSGIDTNGQKQLVIEPEEAKIVRLIYAKYLGGAGYRAIANFLNRQGYQTVK
                     KNAFSTTAVKDILRNKIYAGYLEYARYINWETKRRKGKNPRPILVKGTHEPIISEEDY
                     QKVQERLALEGKQPKWNHTGENVLTGLLRCPECGAPMAASNVTNTLKDGTKKRIRYYS
                     CSVFRNKGASVCHANSIRAEQAETFVADRLKEIVQLPEVLPRLVAALNEEIVRQSQPL
                     EQELVVLLERKEELKTKIEKWEAALEDSPELFPMLKDRLDELTEKRRQLHIRENEILG
                     IFQQGEPIQVKDVQRILTNLDRFLAQSEKKQIKALYRTFIEKITFDPNHKEMLEITMK
                     FTHAVVQQLNEQYQIAVSKTKDTAIFVLKTPFTLTI"
     gene            complement(2186..3559)
                     /locus_tag="IWO89_002509"
     CDS             complement(2186..3559)
                     /locus_tag="IWO89_002509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HAQ0457216.1"
                     /translation="MRVACYDRTSVYEEAKISNDAQYDAVKEYVLEKYEQIPQRFSDK
                     GESGEKAERPAFRQLRAWVCASEEEGVIYVWRYDRLARQAKIALEFIELCQQHQVQVY
                     SISEPLPASFSHASTNKLFVQLLFTFAEQQRNVIIENVRAGLSYKKQQRQYLSSAVPY
                     GYRLVNGKIQQEVHEARIVRYIFQLYLTKKYGYKKLCQRLTQQKLFFRERPFQPYHIY
                     SILKNPLYYGEIKGGSLGKYLGTFEPILSKATFLQAQEIRQSRCTAKKDTYLYLLRQK
                     IRCPFCGRHLSSKYQWNTKKTKTLHYYHCTDRSCQGIFLRAERVESEVLEKIQAFFQY
                     EGVYQALIAQLTQQLAEKRKDEKRKVIQQKKQEAQWIKAFEEGNCSLEELKQQLQALQ
                     KPMSTSSFLSLQQCEAKVKEWLQLKDLSSRQLLCDYVCRIEVTKDKTVCGIYFRQLTQ
                     NILERNE"
     gene            complement(3575..3814)
                     /locus_tag="IWO89_002510"
     CDS             complement(3575..3814)
                     /locus_tag="IWO89_002510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0457217.1"
                     /translation="MTKNKMTLKAEVLLYIQEHFSNQAFFTKPIYLDFEIRGVSAGSI
                     GGTLQALKNEGYLENHFVQRSFNRRVVKKWYLVHS"
     gene            complement(3996..6671)
                     /locus_tag="IWO89_002511"
     CDS             complement(3996..6671)
                     /locus_tag="IWO89_002511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF927 domain-containing protein"
                     /protein_id="HAQ0457218.1"
                     /translation="MKMNHTSIQKHLQLLNTLGISYVNLRTFSTLNKKTVSHIFSTAD
                     SQAIINYLEKQKLSEGTVNITYNPIKQEVITQKKHSVRDNDIEKIRFVFIDIDPKRKE
                     GSATDSEKKKAENVMEQVERYLKEKRIESFVKVDSGNGYHIFLPINEQPNNHETILTI
                     QNFLQLLHRRFGVEDEVDIDTSVYNPSRLCKLVGTPAVKGKSTKERPHRLSSFLSIPD
                     NIDRSNCFKILEQIVEENSMMSTSRVKSHPSFREVLPFIQADYKQWLAHYPELTYTIK
                     EGDHKGVTLLIFDECPLRKHTNNSNGSCLSVTADNKIRFHCLHASDKNKDIQDFVKKY
                     PLPKPVNPSVESLESNQEYYFDQFKLNQEGVFLVKEGQEEKVACPMFIKQIRKNIEQK
                     VITLVLAYYVDHCWDEIEIEGGCLQTNNFKQLAKHGLIIKARREAEVVDFLQIQRENA
                     PIFFEHESLGWKKENEAMIFQLQDSFGREIPSVLTKNSCYQLTTNGTEAEFDALVHKY
                     VLGTNLELAWSIGIASIVLGYLNVTKANTFLSLLINLQGKSTTGKTTTLHFIASLFGN
                     PTTVLRNMNATNKAIIKLASNNQGGVPLILDELGAATQNNFSSLIYQLASGEERLRLD
                     SNCQLMPQETFSVLTLTSSEERLENYFNEKLTGLKVRYIEFSDIKFTKDANHAEKVKS
                     LSSNTFGWGIKLFLLKLFEQGITKIEATFVEAQKKLWAVLPEHSLKQRIGNNLALIYS
                     GALLIQDILGYPLRLEHIQTVLINSYEQLIVDTSLGSEQTVFDRVKTLLVMNSHKFLT
                     NSDNKHTPSTIWGKVSIQKNGDIKVNIFPIPFEDLLRREFQVKDLTYLFRQLLDSGNM
                     CAEKGRRTKRIHMNRQVLPTYEILLPSSLKSYFRL"
     gene            complement(6854..7777)
                     /locus_tag="IWO89_002512"
     CDS             complement(6854..7777)
                     /locus_tag="IWO89_002512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0457219.1"
                     /translation="MATNYAKKINFDVDKLYKSLIVVPKNKSEDGNYELEVLQFKIEQ
                     KSSERFMSNQLVKIKKWLPMIDQLVSNNKEAKNLCIFVLLLHGRVINLVNRKSTTKFA
                     NSFWFLKNINKTVEKNGVYKGDFHFQNAETIEREHHYFDLLVNTVVELAKWAKSNETY
                     SQVLKRIMSQETSFIYELSKSNLKYTYNEDELEILDNPENSQWIKELKREDFIETLRI
                     LKSLFDLKLAKATIMGIERDSYYWEEIGLYAPQIEGEDKEKRIDNIINYINSLSEPLK
                     CRGLDFIGTLGWTETYYEKIYKEIIEQYFPK"
BASE COUNT         2373 a         1595 c         1228 g         2916 t
ORIGIN      
        1 gttagttcat attctcgaat gatttctaca cgtggctttc cagctaagta aagattgacg
       61 atttgttgct tgaattcttg tgaaaaagtt cttcgtgttc tcttagacat aaaaattcct
      121 cctggtatgt tttcttctag tctacacacc ttaatttttc tgtctagtta attgtagcct
      181 atccagtata ataaacattt ccttgaggtt tccttttaat ccccttcgga aaatcacctt
      241 ttgtaaaatc gctcttaagg aaatcatcta aattaccagt tctggtcttt ttacctccag
      301 tattccctga ttttttaggt ggtacatgtt ctttagtaag ttcagtgtat ttttcacata
      361 aaatacactt acccatcttt ttcatatcta tccccactct ttttaattct tatatacaat
      421 tatatttcaa atattcatta ataaatatcc ctatttatga taaggacctc cctaactact
      481 tctctaacga tttcaaatcg ctagagaagc gttagcgccc ctctaacgtt gtttgatata
      541 acctagacta gatcgttaac gtaaagggtg tttttaacac aaaaatagcc gtatcctttg
      601 ttttggatac ggctatttga tattgctcat tcaattgttg taccacggca tgcgtgaatt
      661 tcatcgtaat ttccaacatc tccttatggt taggatcaaa ggttattttc tctataaagg
      721 tacgatacaa ggctttgatt tgcttcttct cactctgggc aagaaaacga tccaaattag
      781 tcaatatccg ttggacatct ttgacttgaa tcggctctcc ttgttgaaag atccctaaga
      841 tttcattttc tcgaatgtgc aactgtcgtc gtttttcagt caattcgtct aagcggtctt
      901 ttaacatcgg aaacaattca ggactatctt ccaaagcagc ttcccatttt tcaattttag
      961 tcttcaattc ttcctttcgt tccagtaaca ccacgagttc ttgttccaat ggttggcttt
     1021 gtcgcactat ttcttcattt aacgctgcca ccaatcgggg aagaacttct ggtagctgaa
     1081 cgatctcctt taatcggtcg gcgacaaatg tttccgcttg ttcggcacga atcgaattgg
     1141 catgacacac acttgctcct ttattccgaa agactgaaca agaataatat cgaatccgtt
     1201 tctttgtgcc atcctttagg gtatttgtga cattactcgc tgccatcggt gcgccacatt
     1261 ctggacaacg taacaagcct gtcaatacat tttcacctgt atggttccat ttgggttgtt
     1321 ttccttctaa agcaaggcgt tcttgtactt tttgataatc ttcttccgaa ataatcggtt
     1381 catgggttcc tttcactaaa atcggtctcg gattcttccc cttccgtcgt ttggtttccc
     1441 aattgatgta gcgcgcatat tctaaatacc ctgcataaat tttattgcgt aagatatctt
     1501 tgactgccgt ggtagaaaaa gcattctttt tgactgtttg atatccttga cgattcaaaa
     1561 aattggcaat cgctcgatac cctgctccgc ctaaatattt tgcatagatc agacgaacaa
     1621 ttttcgcctc ttctggttca atgaccaatt gtttttgacc attcgtgtca ataccactga
     1681 catagcctaa acgtcggcct cctgcataac gctttttatc tttcactaag ctttgcattg
     1741 acatttgaac attctccgca atttggttgc gttcaaattc accgaaactc ccaaggagtt
     1801 gtaacatcaa tttacctgtc gaagtggtca cgtcaaattg ttccgagatc gaatgtaatc
     1861 ctacgtcata tcgcaataat tcctcgactg tttgtaagac atctaacata ttgcgtccga
     1921 gacgggtcaa tttccaaatc actagtagat caaaacgatg gttccgagca tcgttcatca
     1981 ttcgttgata ggcgaaccga ttttttgtgg atttcccact gattccttca tcggcataaa
     2041 cttctaccac ttcatagccc atccgttcgg caaactgtcg tccattttgt tcttgtgctt
     2101 gaatactatg tccttctaaa gcttgttctt gtgtactcac gcgaatatac aatgcgactc
     2161 gtttggtttg tatcatttcc atagatcatt cgttcctttc taaaatattt tgtgtcagtt
     2221 gtcggaagta aatcccacaa actgttttat cctttgttac ctcaatccga caaacatagt
     2281 cacataataa ttgtcgacta gagaggtctt ttagttgaag ccattctttc acttttgctt
     2341 cacactgttg gagtgaaaga aacgaagaag tagacatcgg cttttgaaga gcttgcagtt
     2401 gttgcttgag ttcttccagt gaacaattgc cctcttcaaa tgccttaatc cattgtgctt
     2461 cttgtttctt ttgttggatt actttgcgtt tttcatcttt tcgcttttcg gctaattgtt
     2521 gcgttaattg ggcaatcaag gcttgataga cgccttcgta ttgaaaaaaa gcctgtatct
     2581 tttccaatac ttctgactca accctttctg cccgaagaaa aattccttgg cagcttcggt
     2641 cggtacaatg ataataatga agcgtcttcg tcttctttgt attccattga tacttcgaac
     2701 tcagatgacg accacagaag ggacaccgaa ttttttgacg taataagtac agatacgtat
     2761 cttttttagc tgtacacctt gattggcgaa tctcttgggc ttgtaaaaag gttgctttcg
     2821 agagaatcgg ttcaaaggtt cctagatact tccccaaaga tcctcctttg atttcgccat
     2881 agtacaatgg attttttagg atactataaa tatgataggg ctgaaaagga cgttcgcgga
     2941 agaaaagctt ttgttgcgtc aatcgttgac aaagcttttt gtagccatat tttttggtca
     3001 aataaagttg aaaaatatag cgaacaatcc ttgcttcgtg tacttcttgt tggattttcc
     3061 cattcactaa acgataccca tatggtaccg cagaagacaa atattgccgt tgttgctttt
     3121 tataggaaag tccggcacga acattctcaa taatgacgtt tcgttgttgt tctgcaaaag
     3181 tgaacaataa ttgcacaaat agtttattcg ttgacgcgtg tgagaaagaa gcaggaagag
     3241 gttcagaaat cgaatacact tgaacctgat gttgttggca caactcaata aattctaagg
     3301 ctattttggc ttgacgtgcc aatcgatcat aacgccaaac atagatcacc ccctcttctt
     3361 ctgacgcaca aacccaagct ctaagctgtc gaaaagctgg acgctccgct ttttcccctg
     3421 attctccttt gtctgaaaat cgttggggaa tttgttcata tttctctaaa acatactctt
     3481 tcactgcatc atattgagcg tcattactaa ttttcgcttc ttcataaaca gacgttcgat
     3541 cataacaagc cacacgcatg cttgattccc ctctctatga atgaactaaa taccattttt
     3601 tcacgactct tctattgaac gaacgttgca caaagtgatt ttctaaatat ccttcgttct
     3661 tcaacgcttg aagagtaccc ccgatagatc cagcagatac ccctcttatt tcgaaatcca
     3721 agtaaattgg ttttgtaaaa aaggcttgat ttgaaaaatg ttcttgaata tacagaagaa
     3781 cctctgcttt tagtgtcatt ttatttttag tcattagaat tttcctttct ttcatgtttg
     3841 attgaatgaa gtaaatgaag catagatcaa gcttcattta cttccgtttg ggtttgctcg
     3901 ctatcataca gataagtaat cagttgtttt gctaaatcaa taagaaatat ttccataaca
     3961 tggtgcctca ctttcatact ttggtttatt ctaaattaca atcgaaaata cgatttgagt
     4021 gaagaaggta atagaatctc gtatgttgga agaacttgac gattcatgtg aatacgtttc
     4081 gttcttctcc ctttttctgc acacatatta cccgaatcaa gcaattgcct aaaaagatag
     4141 gttagatcct ttacttgaaa ttctcgcctt aacaagtcct caaacgggat aggaaaaata
     4201 tttaccttaa tatccccatt tttttgaatt gatacttttc cccaaatcgt actgggcgta
     4261 tgtttgttgt cactgtttgt taaaaattta tggctattca tcaccaacaa tgttttcact
     4321 cggtcaaata ccgtttgctc acttcctaaa ctagtatcta caattaactg ttcgtacgaa
     4381 ttaataagca ccgtctgtat atgctctaat cgtaaaggat agcccagtat atcttgaatt
     4441 aggagggctc cactataaat caaagctaga ttattaccaa tgcgttgctt caaagaatgt
     4501 tcaggtaaaa cagcccatag ttttttttga gcttcgacaa aagttgcttc aatttttgta
     4561 atcccttgtt caaagagttt caaaagaaat aactttatac cccaaccaaa agtattagaa
     4621 gaaagacttt taactttctc tgcgtgattc gcatcctttg taaacttgat atcagaaaat
     4681 tcgatatatc gaactttcaa accagttaat ttctcattga aataattttc caaacgctct
     4741 tcagaagagg taagggtcaa aacagaaaaa gtctcttgtg gcataagctg acaattagaa
     4801 tctaatctta gcctttcttc tcctgaagct aattgataga tcaacgagct aaagttattt
     4861 tgagttgctg ctcctaactc atctagtatt agtggaacac ctccctgatt gtttgatgct
     4921 agctttataa tggctttatt ggtagcattc atatttctaa gaaccgtagt tggatttcca
     4981 aataaagagg caataaagtg taacgtagtg gttttacccg ttgtgctctt gccctgtagg
     5041 ttaattagta aactcaaaaa agtattagcc ttcgtaacgt ttaaatagcc taacacaata
     5101 gaagcaatac caatcgacca agcaagttct aagttcgttc ctaaaacata cttatgaacc
     5161 aatgcgtcaa attccgcttc tgtaccatta gttgttaatt ggtagcatga gtttttagtt
     5221 aatacagaag gaatttcacg tccaaaagaa tcttgtaact gaaaaatcat tgcttcattt
     5281 tcttttttcc agccaagaga ttcatgttca aaaaagattg gagcattctc ccgttgaatc
     5341 tgtaaaaagt caacgacttc agcttctctt ctagctttaa tgattaagcc atgttttgct
     5401 aattgtttaa aattattagt ttgtaaacat ccaccttcta tttctatttc atcccaacaa
     5461 tgatcgacat aataggcaag aacaagtgta atgacttttt gttcgatatt tttgcgtatt
     5521 tgcttaataa acatcggaca agcaactttt tcttcttgac cttctttcac caaaaatact
     5581 ccttcttgat tcagtttaaa ctggtcaaaa taatattctt gattggattc taatgattct
     5641 acagaaggat ttacaggttt tggcaacgga tattttttta caaagtcttg aatatcttta
     5701 tttttatccg aagcatgcag acaatgaaat cgaatcttat tatcagccgt aacagacaaa
     5761 caagaaccat tagaattatt cgtgtgtttt cttaatggac attcgtcaaa tataagtaag
     5821 gtaactcctt tatgatcgcc ctcttttatg gtataagtta attcaggata gtgagctaac
     5881 cattgtttat agtctgcttg aataaaaggt aacacttctc taaaagatgg atggcttttt
     5941 actctagagg ttgacatcat ggaattttct tcaactatct gttcaagaat tttaaaacaa
     6001 ttacttctat ctatgttatc gggaatagaa aggaaactag ataaacgatg cggacgctct
     6061 tttgtggact ttcctttgac cgctggtgta ccaactaatt tgcataatct tgaaggatta
     6121 taaacagaag tatcaatatc aacttcatct tctactccaa atcttcggtg aagtaattga
     6181 agaaaattct gaatagtaag aatcgtctcg tgattatttg gttgttcatt tatgggaaga
     6241 aaaatatggt agccattccc actatcaact tttacaaatg attctattct cttttctttc
     6301 aaataacgtt ctacttgttc cataacattt tctgcctttt tcttttcgct atctgttgca
     6361 cttccctctt tccttttagg gtcaatatca ataaatacaa accgaatttt ttcaatatca
     6421 ttgtctctca cagaatgctt tttttgtgta atcacctctt gtttaatagg attataagtt
     6481 atgtttactg taccttcaga caatttctgt ttttctaaat agttaataat tgcttgagaa
     6541 tctgcagtag aaaaaatatg agaaacggtt ttcttgttta atgtagaaaa agttctcaaa
     6601 ttaacataag aaatccccag tgtatttaac agttgtaaat gcttctgaat tgaagtgtga
     6661 ttcattttca ttaattacca ctcactttct aaaaaatttt tatttgtatg actacattac
     6721 ctccaacctc tcataatttc atgatcttca gttctacaaa aatatatccc actcaaaatc
     6781 ttgacaaaag aaaaaagttg tgattttgga aatcacaact tttttctttt acgataaatc
     6841 aatccttatt aaattattta ggaaaatatt gttcaattat ttctttatat attttctcat
     6901 agtatgtttc tgtccatcct aatgttccta taaaatcaag tcctctacat tttaaaggtt
     6961 cacttaaact attaatatag ttaataatgt tgtctatcct tttctcttta tcttcacctt
     7021 caatctgggg agcatatagt ccaatttcct cccaataata tgaatctctt tcaattccca
     7081 tgatggtagc tttggctaat ttcaaatcaa ataacgattt tagaattctt agtgtctcaa
     7141 taaagtcttc tctcttcaac tctttaatcc attgtgaatt ttctggatta tctaaaattt
     7201 caagttcatc ttcattataa gtatatttca aatttgactt gcttaactca tagataaatg
     7261 aagtttcttg actcataatt cttttaagta cttgactata agtttcgttt gactttgccc
     7321 atttcgctaa ttctaccact gtattcacta ataaatcgaa ataatgatgt tctcgttcaa
     7381 tagtctcagc attttgaaaa tgaaaatcac ctttataaac tccattcttt tccactgttt
     7441 tattaatatt ttttaaaaac cagaatgagt ttgcaaattt agtcgtactt tttcgattaa
     7501 ctaagttaat aactcttcca tgtaataata agacaaaaat acataaattt ttagcttcct
     7561 tattattaga tactaattga tcaatcatag ggagccattt tttgattttc actaattgat
     7621 tgctcataaa tctttcactg gacttttgtt caatcttaaa ttgcaaaact tctaactcat
     7681 aattcccatc ttctgattta tttttgggaa caactatcaa acttttataa agtttatcca
     7741 catcaaaatt tatctttttt gcataatttg tagccattaa tacatctcct tattcataat
     7801 aaatttcata tgacgctcct ctcaaaattt accggtagac tagaaaaaag aataaaagat
     7861 acgtcaattc acactttatt cttaaaaaaa aactggtctc tctaccggta atatcgattg
     7921 agtccgcctt tatcgacttt atcctttttt tatattaaat tttttcaccg gtagacaggg
     7981 agagaaagaa taaactaaaa agcccttaat tcttaatgtt ttcacctcct taccgttcct
     8041 accgctaaaa tttaatataa ctaaattcta taacattaga agaaatttta aatacctttg
     8101 ataaaaaata tt
//
LOCUS       DACEEZ010000104         7694 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20313_2017
            SAMN12639966-rid9879223.denovo.104, whole genome shotgun sequence.
ACCESSION   DACEEZ010000104 DACEEZ010000000
VERSION     DACEEZ010000104.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639966
            Sequence Read Archive: SRR10023158
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7694)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7694)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741683.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741683.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:44:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,016
            CDSs (total)                      :: 2,962
            Genes (coding)                    :: 2,889
            CDSs (with protein)               :: 2,889
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 36 of 73
            Pseudo Genes (incomplete)         :: 29 of 73
            Pseudo Genes (internal stop)      :: 18 of 73
            Pseudo Genes (multiple problems)  :: 8 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7694
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639966-rid9879223.denovo.104"
                     /isolate="E20313_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(25..648)
                     /locus_tag="IWO89_002513"
     CDS             complement(25..648)
                     /locus_tag="IWO89_002513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0457220.1"
                     /translation="MRKKRPFLLFWLFGFFLLLILSAAFSGKMTADLAQKETEKIVAS
                     KESVDNFKKEFSTYWWAEYKITANYEKGELDIYLPKGKEENQFINIDDVLSAISLRQY
                     QFKDKHLTVLLLTHDGDKVARLKNGKVITAYSEKVDKKVVADRLAEWKKTNEQVKKTT
                     VSSQKNESTGTSPSDSGSGQTITGSEENQASIITNESSQGQYQSYGQ"
     gene            complement(807..2204)
                     /locus_tag="IWO89_002514"
     CDS             complement(807..2204)
                     /locus_tag="IWO89_002514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HAQ0457221.1"
                     /translation="MKKYQFPANFWWGSAASGPQTEGRISGDGKGENIFDYWYKKEPQ
                     KFFDQVGPEKTSQVYTKYQEDVQLMKQTGHNSFRTSIQWSRLIPDESGTINEEAVRFY
                     HRYFDELIENGIEPFVNLYHFDMPMYLQEKGGWLNRETVEAYEKYAKSCFELFGSKVK
                     KWFTHNEPIVPVEAGYLYRWHYPEESDMKKAIQVGYHEALASALAIKSYHEMEQGGVI
                     GIILNLTPSYPRDESNPEDVKAAQIADAFFNRSFLDPAVKGEFPSELIEIVKELDLMP
                     VIHAEDLTVIKENTVDLLGINYYQPRRVKAKETPIDTKNGPMPEDYFDNYEMPGRKMN
                     PYRGWEIYEQGIYDILINVRDHYGNIDCFISENGMGVENEARFIKEDGMIHDDYRIEF
                     VQSHLKYVHQAIQEGANCLGYHMWTCMDNWSWTNAYKNRYGFISVDLAQDGKRTIKKS
                     GHWFKDVSDKNGFEA"
     gene            complement(2236..3207)
                     /locus_tag="IWO89_002515"
     CDS             complement(2236..3207)
                     /locus_tag="IWO89_002515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family transcriptional regulator"
                     /protein_id="HAQ0457222.1"
                     /translation="MASIRDVAKMAGVSVGTVSRYLNGQQLKEKNMKKIAEAISALDY
                     KENIIAKGLKNNRSFSIGLLINGISSRFGSEVVSGIERVAEENGYSLLLSGFADQPEK
                     IEQKIEYLMKHVIDGLIVFLSEEEWSGFEMLTKMSIPVIALNCPEGPAGIDTILVNDR
                     ESVAKVIAHHGEQGHKKIGFIAATQTDYVARERLAGAKEAVQNDPSIQLEVFTGDYSR
                     KSGYYGAKALLEKGATAIFVSNYNMSIGAIECFNQEGVRIGKDIAFSHYDYLDKNDLS
                     ILPKITITPPTDKIGERAAEQLLKRIKKEEEENEKVIVMENIIHGIN"
     gene            3581..4006
                     /locus_tag="IWO89_002516"
     CDS             3581..4006
                     /locus_tag="IWO89_002516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0457223.1"
                     /translation="MKKLIVLFILSAGLLTLTGCDLFGGSDQSSDETTDSSVLKAEKA
                     DTTNSVYTLKVRKDMDLSAGQLTFTDKKMEWTRTYQTQGTDSSESSEEKTVLTDIKIT
                     TKDDEYIISGKENKKETTVIFTKIGNYRLKDEDGNIYSL"
     gene            complement(4494..5195)
                     /locus_tag="IWO89_002517"
     CDS             complement(4494..5195)
                     /locus_tag="IWO89_002517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HAQ0457224.1"
                     /translation="MSVILISGTIGAGKSSLTDMLAKEIDSKPFYENVEDNEVLPLFY
                     SNPEQYAFLLQIFFLNKRFLAMKNALVNDDNVLDRSIYEDSLLFHLNADLGRVTDIEV
                     QQYDSLLDTMLNELDDVAPKKRPDLMVHIKVSLDTMLERIKKRGRDYEQLESDETLYT
                     YYETLNTRYNQWYEDFDVCPKIQVDGDKFDFVENPEDAKKVIRQIQEKLAELEGKPAG
                     KYFTDNQHAKGQELG"
     gene            complement(5211..5651)
                     /locus_tag="IWO89_002518"
     CDS             complement(5211..5651)
                     /locus_tag="IWO89_002518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside deoxyribosyltransferase"
                     /protein_id="HAQ0457225.1"
                     /translation="MSQIYLAGPFFSEEQIDRVSRIEKALEENKTVTSFYSPRHHQES
                     NYELFSAGWAQEVYEKDMEELTNAEFVVAILDFEHQTIDPGTAYELGVATMLKKPMII
                     VQEETVPTNLMITQSLHTYLKSDQAVREYDFETLPVETYVGEYL"
     gene            complement(5651..6226)
                     /locus_tag="IWO89_002519"
     CDS             complement(5651..6226)
                     /locus_tag="IWO89_002519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00730 family Rossman fold protein"
                     /protein_id="HAQ0457226.1"
                     /translation="MKLTVFCGSRFGNKESYKFIAQTLGKYMAQEEIELVYGGSYSGI
                     MGVISKTVLENNGKVTGIYPNGLFETELPGKDVTTFIPTETIDERKVLLFEKGDAVLV
                     FPGGLGTLEEFSQLLSWIAIGLTPDKPIGILNIGGYYSGLQQLLETFAKEDFMDKKWL
                     DQVIFSNNPLELVNELRAVSIKNQLLLKEAK"
     gene            complement(7074..7553)
                     /locus_tag="IWO89_002520"
                     /pseudo
     CDS             complement(7074..7553)
                     /locus_tag="IWO89_002520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015510350.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIC"
BASE COUNT         2195 a         1597 c         1230 g         2672 t
ORIGIN      
        1 cgctgatttc acaacctctg tttttcattg tccataggat tgatattgtc cttgtgatga
       61 ctcattcgta ataattgagg cttgattttc ttcacttccc gttatcgttt gtccactgcc
      121 agaatctgaa ggacttgttc ctgtcgactc gtttttttga gaagagacag ttgttttctt
      181 gacttgttca tttgtctttt tccattccgc taaacgatcg gcgaccactt ttttgtctac
      241 tttttcagaa taagcggtga tcacttttcc atttttcaaa cgggctactt tgtcgccatc
      301 atgagtcaaa agcaaaacgg tcaaatgctt gtctttaaat tgatattgtc tgagagaaat
      361 cgcactaagt acgtcatcga tattaataaa ttgattttct tcttttcctt ttggcaaata
      421 aatgtccaat tcgccttttt cgtaattggc agttatttta tactccgccc accaataagt
      481 tgagaattct tttttgaaat tatctactga ttctttgctt gctacgattt tttctgtttc
      541 tttctgagca agatctgctg tcatttttcc tgaaaatgct gcggataaaa taagcagtaa
      601 gaaaaaaccg aataaccaaa agagaagaaa cggtcttttt ttcctcatgc tttcctccac
      661 tcctatttaa ttttgactat tctattatat acaaaaaatg aaatagaaag acaaaaaagt
      721 tgaaaaagat cgtaaataat tgaacttctt tttcacaaaa tggtaagaat ggagaatttc
      781 acgcatttgc tctcaactat tctttcttaa gcctcaaatc cgtttttatc tgatacgtct
      841 ttgaaccaat gacccgattt tttgattgtt ctttttccat cttgtgctaa gtctactgaa
      901 ataaagccat atcgattctt atatgcattg gtccacgacc agttatccat acatgtccac
      961 atatgatagc ctaaacaatt ggcaccttct tgaatggctt gatggacata tttcagatga
     1021 gattggacaa attcgatccg ataatcatcg tggatcatgc catcttcttt gataaatcgt
     1081 gcttcgtttt ctacacccat accgttttca gaaataaagc agtcgatatt tccataatga
     1141 tctcgtacat tgatcaaaat atcatagatt ccttgttcat agatctccca tccgcgataa
     1201 ggattcattt tacgaccagg catttcataa ttgtcgaaat aatcctcagg cattggaccg
     1261 tttttagtat cgattggtgt ttcttttgct tttacccggc gtggctggta ataattgata
     1321 cctaaaagat ctactgtatt ttctttgata accgtcaaat cctctgcatg gatcacaggc
     1381 atgagatcaa gttcttttac tatctcaatc aattcacttg gaaactcgcc ttttacagct
     1441 ggatcaagga atgaacgatt gaagaacgca tctgctattt gcgctgcttt aacatcttca
     1501 gggtttgatt cgtctcttgg atagcttggt gtcagattca agatgatacc aataacacca
     1561 ccttgctcca tttcatggta agatttgatg gctaacgctg aagccaacgc ttcatgatag
     1621 ccaacttgga tcgctttttt catatctgac tcttctggat aatgccatcg atataaataa
     1681 cctgcttcga ctggaacgat tggttcatta tgagtaaacc atttttttac tttcgaacca
     1741 aacaattcaa aacaactttt ggcatatttt tcataggctt caaccgtttc tcggttcaac
     1801 catccgcctt tttcttgcag atacatcggc atatcgaaat gatacagatt cacaaagggt
     1861 tcgataccgt tttcaatcaa ctcatcaaaa taacgatgat agaaacgaac ggcttcttcg
     1921 ttgattgttc cagattcatc aggtatcaaa cggctccatt ggatagatgt tcgaaatgaa
     1981 ttatgcccag tctgtttcat caactgtaca tcctcttgat acttcgtata aacttgcgag
     2041 gttttttcag gtcctacttg atcaaagaac ttttgcggtt cttttttgta ccaataatca
     2101 aaaatgttct ctccttttcc atcgcctgaa atccttcctt ctgtttgtgg gccacttgct
     2161 gcagaacccc accaaaaatt tgctggaaat tgatattttt tcatcaaagc acctcttttt
     2221 cttttttagt tcgttttagt tgattccatg tatgatattt tccatcacaa tcactttttc
     2281 attttcttcc tcttcttttt tgattcgttt taacagctgt tctgctgctc gttccccgat
     2341 tttatctgtt ggtggcgtga tcgtgatttt cggaaggata ctcagatcgt ttttatctaa
     2401 ataatcatag tgactgaacg caatatcctt cccgattctg acaccttctt gattgaaaca
     2461 ttcgatggca ccaatcgaca tattgtaatt gctgacaaaa atagcagttg cccctttttc
     2521 taataatgct tttgccccgt aatagccgct ttttctagaa tagtctcctg taaagacctc
     2581 taattggatc gatggatcat tttggaccgc ttctttggct cctgctaaac gttctcttgc
     2641 tacgtagtct gtttgagtgg cagcaataaa tccgattttt ttatgtcctt gttcaccatg
     2701 atgagcgatc acttttgcta cactttctcg atcattcact aatattgtat caatacctgc
     2761 tggtccttct ggacaattca atgcgataac tggaatactc atttttgtga gcatttcaaa
     2821 tccagaccac tcttcttcgg ataagaagac aatcagccca tcgatcacat gcttcatcag
     2881 gtattcaatt ttttgttcaa ttttttctgg ctgatcggca aacccgctta ataataagct
     2941 atacccattt tcttcagcta ctctttcgat ccctgagaca acttccgacc caaaacggct
     3001 ggaaatcccg tttatcaaaa gtccaataga gaagcttcga ttattcttca accctttagc
     3061 aatgatgttt tctttatagt ccaacgctga aatcgcttct gctatttttt tcatgttctt
     3121 ttccttcagt tgctgaccat tcaagtaacg ggaaactgtt cccactgaaa caccagccat
     3181 cttagccaca tcacggatcg atgccataca ttctcactcc tgccagttac atttgatttc
     3241 tctttcttgt tgttcgatta tgatgaccgc attctttgca tagtgttgct tgtcatcaat
     3301 atccatataa ctaaagtctt cagtagagca acttcctctc actattaatt atacttaaac
     3361 aagtatttga accggttcat ttttaatata gcattttttt aaaagattgc aacacttttt
     3421 taaaacgctt tctgaaagtg gcggattttt tgccttttta aaattttatt ctaaaattta
     3481 ctctatttgt gggtgttttg tgcgtttttc tcttccatcc gtttgaagtt ttacattcca
     3541 atggtataat ttctttatcc tatcaaatgg gggaagtatt ttgaagaaat taatcgtact
     3601 atttattttg tctgcgggac ttttgacatt gactggctgt gatttgtttg gcggctctga
     3661 ccaatcctcg gacgaaacaa ccgattcttc tgtcttaaaa gcggaaaaag cagataccac
     3721 caacagtgta tatacgctaa aagtacgtaa agatatggat ttatccgcgg gacagttgac
     3781 atttacagat aaaaaaatgg aatggacacg cacgtatcag acacaaggaa ctgattcttc
     3841 tgaatcatct gaagaaaaaa cagtgctgac agatatcaag atcactacca aagatgacga
     3901 atatattatt tcaggaaaag aaaataaaaa agaaacaaca gtcatattta caaaaatcgg
     3961 taattaccgt ttgaaggatg aggatggcaa catctacagt ctatagtgcc gtctgttgga
     4021 tcttcttttg tttctcccct tcttttctgc tatagtaaag aagcaagatg attgtaaagg
     4081 agacgaaatg gatgatcgat agtcctgatt attgtacatg taataacaac aaaaatatcc
     4141 atacagaatt taatgaccaa ggaagcttga gcatatgcga caactgtcac aaactagtca
     4201 aagattcatt taaaacattt gaatcttatc ctgttcaaaa ttatgtaaaa agtagaagaa
     4261 gataaacaaa tccaagcaaa actcactatg gctgattttt atgaacttca aactcagctt
     4321 cttcctttga tcaagcttca tcagtaaaac aaacagtgga tcatttcagt ttacaaatca
     4381 aagagtaaag atttatttat ttcaaatatt tgaatataaa ataaccaaac gatagaaagg
     4441 cttattctac atcacgtagt aatttttctt tcccgtcgtt tggtttttta tttttagcct
     4501 aattcttgac cttttgcatg ctggttatcc gtaaaatatt ttccagcagg ctttccttcc
     4561 aattcagcta atttttcttg gatctgacgg atcacctttt ttgcatcttc tggattttct
     4621 acaaaatcga atttatcccc atccacttgt attttaggac atacatcaaa atcttcatac
     4681 cattgattat agcgggtgtt caacgtttca taataggtat ataacgtctc atcgctttct
     4741 agctgttcat agtcacgccc acgttttttg atacgttcta gcatcgtgtc caaagaaact
     4801 ttgatatgga ccatcaaatc aggccgtttt tttggagcaa catcatctaa ctcgtttagc
     4861 atcgtatcta ataagctatc gtactgctgt acttcgatat ccgttacccg acctaaatca
     4921 gcgtttagat ggaacaacag gctatcttca tagatggatc ggtctagcac attgtcatcg
     4981 ttcactaaag catttttcat tgctaaaaat cgcttgttca aaaagaagat ttgcaataaa
     5041 aaagcgtact gctcaggatt tgaataaaag agcggcaata cttcgttatc ttcgacattt
     5101 tcataaaatg gtttagaatc aatctctttt gcaagcatat ccgtcaaact gctcttacct
     5161 gcaccaatcg ttcctgaaat caaaatcact gacatacaac ccttcctctt ctataagtac
     5221 tcgcctacgt aagtttcaac aggcaatgtt tcaaaatcat attcacgtac agcttggtct
     5281 gattttaaat acgtatggag actttgtgta atcatcaagt tcgtaggtac cgtctcttct
     5341 tgtacaatga tcatcggttt tttcaacata gttgccacac ctaattcgta agccgttcct
     5401 ggatcgattg tttgatgctc aaagtcaagg atagcaacaa caaattctgc atttgtcagt
     5461 tcttccatat ctttttcgta tacttcctgc gcccatccag cgctgaatag ttcataattg
     5521 ctttcttggt gatgacgtgg agagtagaaa cttgtgactg ttttgttttc ctccaacgct
     5581 ttttcgatac gacttactcg atcgatttgc tcctctgaaa aaaatggtcc tgctaaatat
     5641 atttgactca ttattttgcc tccttcaata ataactgatt tttaatagat actgctcgta
     5701 attcattgac cagttccaat ggattgttgg aaaagattac ttgatccaac cattttttat
     5761 ccataaaatc ctcttttgca aaagtttcta ataattgttg caagccgctg taataaccgc
     5821 caatattcaa tattccgatt ggcttgtcgg gcgtcagacc gattgctatc caagaaagaa
     5881 gttgtgagaa ttcctccaat gtacctagac ctccagggaa aacgaggaca gcatctccct
     5941 tttcaaataa taataccttt ctttcatcga tcgtttctgt aggaataaat gtagtaacat
     6001 cttttccagg caattcagtt tcaaaaagcc cattcggata gatacctgtg acttttccgt
     6061 tattttccag tactgtttta ctaatcactc ccataatccc ggagtatgaa ccaccgtaaa
     6121 ccagttcgat ttcttcttgt gccatgtatt ttcctaacgt ttgcgcaata aatttgtaac
     6181 tttctttatt accaaaacgg gaaccgcaaa aaacagtaag tttcataaaa aaacactccc
     6241 taactcaaaa gtctatcttc attctacacg tgttttttta aaataaaaga ctagattttg
     6301 tagtttttta gtggtatata ggatagacgc cactatatat agtatttaga aaaaaatcat
     6361 ttttcagcca ttttatagat aaaacaaaaa taaataacgt gttttcatcg atatgaaatg
     6421 acttacttat agaagatact gggatttcgt cctatatgcc aataacgtgt attattttaa
     6481 acaacaaata cttgtttctt ttattaacga aaaaatcatt ttgttctcgt gttatgtttc
     6541 actgataaat gatgtttctc taaataaaaa agccgacgaa ctaaacgatc aaaatttgat
     6601 catttttgtt catcagcttc tcctaaaaat ctttttgtta acgataaata gctggatttg
     6661 taaaaatatc acttgaatcg tcactgctgg aagaaaattc agtgataacc gcacctgttg
     6721 cgcctgcctg gaactagtgt ttgatattgg gctcgaacgt gtactgatct ctagcatgaa
     6781 gaataatttc tttttttact gtataataac tttctttcct ttttggaata gtacaggaaa
     6841 gtttttttgt ttcctttcct tcgacataag gtataccaga ccctttcgac aacgaaaggt
     6901 tctcattttt ccttcatcgt catttcaagg agggtgccga tgctctggac agtttcgatc
     6961 gctcctaaac caaaatctgt atattcgatt ctttctaact ctgattcttt cataccattc
     7021 ctgtctcaag gtacatctat tgtacttttt tcttgtaatc cattctgcct ctcctaacta
     7081 attgtcaaat catcccaaga tatgtcttct tcctcttttt cttcttggat gatttctgca
     7141 ttcggatcta ctggtttttt caataataaa atggttattc ctgtgacaaa agaaccagct
     7201 aacaatgcac caatgaatgc ccatggtctg gtcatcgtag cgactgcaaa cattcccgat
     7261 gccggaatac ggctatctgc gccccaaaac atgcttaatc cgccacctac tgtcccaccg
     7321 actcccgttg ctatgacatt gcgtgccaag tcgttcaata cgatagggaa acatccctct
     7381 gtgatctgac agatccccat aggaaaagcg gtcttcaatg tatcgatttc ttgacgattg
     7441 agtatatttt tacgaatgat tttccctaag aaataagcga tcgtcaagcc gaatggggtt
     7501 accatcgaag ctaaaatcaa tacagtgatt ggttcataga ttccttctga aaatcgtgcc
     7561 acaccccctt atccagaata aatggtaatc cagaaacagc cgtgtatctg cggtaataag
     7621 cctaaccaaa cgaaaaatat gagaagctat ttttgtttgg ctgttcttat tacttgatgc
     7681 agggcagctt tttc
//
LOCUS       DACEEZ010000105         7666 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20313_2017
            SAMN12639966-rid9879223.denovo.105, whole genome shotgun sequence.
ACCESSION   DACEEZ010000105 DACEEZ010000000
VERSION     DACEEZ010000105.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639966
            Sequence Read Archive: SRR10023158
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7666)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7666)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741683.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741683.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:44:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,016
            CDSs (total)                      :: 2,962
            Genes (coding)                    :: 2,889
            CDSs (with protein)               :: 2,889
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 36 of 73
            Pseudo Genes (incomplete)         :: 29 of 73
            Pseudo Genes (internal stop)      :: 18 of 73
            Pseudo Genes (multiple problems)  :: 8 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7666
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639966-rid9879223.denovo.105"
                     /isolate="E20313_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(<1..732)
                     /locus_tag="IWO89_002521"
     CDS             complement(<1..732)
                     /locus_tag="IWO89_002521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAQ0457227.1"
                     /translation="MIELRDVSVCFKQKEKEIQAVDSVDLTIEKGDVYGIVGYSGAGK
                     STLVRVMNLLQKPTAGEVIVNQTNLSQLSPKELRKERKAIGMIFQHFNLMESRTIFDN
                     VDFSLKYSGKSKQERRQKVNELLELVGLEEKASAYPKQLSGGQKQRVAIARALANEPK
                     VLLCDEATSALDPKTTLQILALLKKLNRQLGLTIVLITHEMQVVKEICNKVAVMEDGR
                     IVEKGSSIQIFSNPEEELTKDFIRTA"
     gene            complement(1379..2050)
                     /locus_tag="IWO89_002522"
     CDS             complement(1379..2050)
                     /locus_tag="IWO89_002522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HAQ0457228.1"
                     /translation="MKGAIFDLDGLLVDTEKTYRDGWLWGFQQFDIHIPKEIVDAWGG
                     KNWKQSFDILVKAAGSPEKVQEVRAKREEYFYQQLTSGAIHLKPYAKEILADLKERHM
                     KLGIATTTVTKRATDILDHFKLADYLDTFTFGDEVSENKPSPIPYLTALERTGLEASS
                     AFAVEDSLVGATAASRAGLGVVLIPDSSFVRNYTVEEKEKLKLLAEGKDLRTVIEMLN
                     KKTEK"
     gene            complement(2137..4815)
                     /gene="mgtA"
                     /locus_tag="IWO89_002523"
     CDS             complement(2137..4815)
                     /gene="mgtA"
                     /locus_tag="IWO89_002523"
                     /EC_number="7.2.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium-translocating P-type ATPase"
                     /protein_id="HAQ0457229.1"
                     /translation="MSFGHKGEIEMEKKKDTQKIYAAFAGKTKQELFELFHASANGLT
                     EEQIERSRENYGENAISYGKKTPFIVEILKAYITPFTLVLIALGIISFITEYVLAAPG
                     DKDLFGVAIIFTMVIVSGTMTLIQSVRSNQAAEKLKSLVKVTAAVKRNGKYTEIPMEE
                     IVCGDLVRLSAGDMIPADMRLLSAKDLFISQAALTGESYPVEKHAKVCEDDQQSETSY
                     ENLVFMGSNVISGSAEGIIVSVGNETLFGHVAETLSEKPIQSSFELGIHKTSMLLIKF
                     MSLMAPAVIVINGLTKGDWLEAFLFGLSVAVGLTPEMLPMIVTTNLVKGASVMAKKGT
                     VIKNLNAIQNFGAIDVLCTDKTGTLTQDKIILEYHMDCEGNEDDRVLRHAYLNSYYQT
                     GLKNLLDVAIIDEATETLDTDKINYQKVDEIPFDFERRRMSVVVADPAGKTQMITKGA
                     IEEMLNISKFIDLGGTVTPLTKERKEAVLAKVQSLNEDGLRVIGVAQKTNPSVVGEFS
                     VKDESDMVLIGYLAFLDPPKETTKQALKALKEHGVAVKVLTGDNELVTRSVCRQVGLE
                     INELVTGEKISEMSDHELAQVAEDHEVFVKLNPQQKARLTTALRKNGHTVGFLGDGIN
                     DAPAMKVADIGISVDTAVDIAKESADVILLEKDLTILERGLLSGRETFGNIMKYIKAT
                     ASSNFGNMFSVLIASTFLPFLPMLPLQLLFLNLIYDVSCISLPWDRMDKEYLDEPKKW
                     NASSIGEFMVYFGPTSSIFDITTYLLMYFVICPAVVGGSFHTLDSSQRVIFIALFHAG
                     WFVESLWTQTLVLHALRTPKVPFIQSNATFAMFVITTLGIIVGSVLPFTSFGAELGLM
                     PLPGNYWLWLFATVIAYLALVTAVKKIYIRRFQELL"
     gene            5474..5911
                     /gene="ndk"
                     /locus_tag="IWO89_002524"
     CDS             5474..5911
                     /gene="ndk"
                     /locus_tag="IWO89_002524"
                     /EC_number="2.7.4.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="HAQ0457230.1"
                     /translation="MERTLVIIKPDGVRRHLVGSIIQRFEAKGLAIAEMKFETMTPEL
                     AKEHYQHLTERSFFDELIDYMTSGPVVYLVLVGEEVIDIVRKMVGATKAADAVPGTIR
                     GDYALPGTENIIHASDSRDAAVKEIARFFPPFDINKPSEKAAK"
     gene            complement(5933..6799)
                     /locus_tag="IWO89_002525"
     CDS             complement(5933..6799)
                     /locus_tag="IWO89_002525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HAQ0457231.1"
                     /translation="MYDGELIKKLRVNKKLTQSQLAEGICFKTSLVGIETNSVKKMSF
                     VTLRSFLERMNMTLAEYEWMRNQLDEPIKLKKKRRMLDRVQEDSFDPYKEISNNRRHF
                     KKTEDLYYLVLNLHMVEKTEVSETEFEFLRYECRIIEEYLMQIPDYGQFELSILADFP
                     WIFSDRFIENNYLKIKKRMRRLAQLNNHEQYLFTFLMNLTSFYIENGRLKKARSVNED
                     MKRSLAHKERSTVIYETLMADYYQRLISAALGEEKTKDYQEFFTVLEYMLGKNQRRSL
                     EKKLLAIAEPSN"
     gene            complement(6959..>7666)
                     /locus_tag="IWO89_002526"
     CDS             complement(6959..>7666)
                     /locus_tag="IWO89_002526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HAQ0457232.1"
                     /translation="YLKKSGIYQNSIFVIYGDHFGISNTDNKDLASALGKDPDTWDEF
                     DNAQMQRVPLMIHMPGYTKGTVNHEYGGEIDVLPTLLHLLGIDDKDYLHFGTDLLSSQ
                     HDQVVAFRNGNFVTPKYTVLGGKAYNNQTGEIIDTKAAGIDEEISKDQEKVKTALSLS
                     DKLNQENLLRFYVPNGFETIDPKKYDYKNEAERNEAIEKQKGEHSTSVFSKNGNKTTT
                     NLYPVTRTDVEQQKNNQ"
BASE COUNT         2165 a         1659 c         1279 g         2563 t
ORIGIN      
        1 agctgttcga ataaaatctt tggtcaattc ttcttcagga ttgctgaaga tctggatgct
       61 gctacctttt tcaacgattc ttccatcttc cataaccgct accttgttgc agatttcttt
      121 gacaacttgc atctcatgag tgatcaggac gattgttaaa cctaactggc gattcaattt
      181 ttttaacaat gcaaggatct gaagggtcgt tttcggatca agtgcactgg ttgcttcatc
      241 acataataat accttaggtt cattcgccaa agctcgtgcg attgctactc gttgtttttg
      301 tccccccgac agttgtttag gataagcaga tgctttttct tccaaaccga caagttccag
      361 tagttcattc actttttgac gacgttcctg tttcgatttt ccagaatact ttaaagaaaa
      421 atcaacgttg tcaaaaatag ttcggctttc cattagattg aaatgctgaa agatcatacc
      481 gatggccttt ctttcttttc gcaattcttt gggagagagc tgactcagat tcgtctgatt
      541 gacaatgact tctcctgctg ttggtttctg cagcaaattc atcacacgaa ctaacgtact
      601 tttccctgca cctgagtaac cgacgattcc ataaacatcg cctttttcaa tcgttaaatc
      661 tacagaatct actgcctgta tctctttttc tttttgtttg aaacatacag aaacatctcg
      721 caattcaatc attaaaattc ctccttatct aaaaaaagcg tcctcaagaa aatgaaaatg
      781 tcattttctt gaggacgctt ttttgcgtgt taccacctca tgttcaagct gtgttcacac
      841 acaactcctc ttcaagtacg atcatactct agcaagatat cggtcgcagc cgtcatcgcc
      901 ttacatctat gctcgggatg aattgctcga tggccatttt ccaatagtcg ttccttgctt
      961 cttctcagct accgaagctc tctgtcaaag gttcttctat cgtacttacc atttcaatgc
     1021 aaataatatt caaaaaaaaa gtccttttac taacataaac tatgtcagta aaaggacgac
     1081 gtatcgcggt accacctttt ttcggaagtt tccttccctc aagcccaaca tctcaaaaag
     1141 agagattggc gttcgtttta acggtcgaac agttccgaat ttgcctactt atcgacaaat
     1201 tattctccaa ggccattttc agcttttctt acttgactct tctcaccagc cagagcctct
     1261 ctgcaaagtt tccaaactta ctttcctttt caacgaaatc gatattttgt tgttgggatt
     1321 aatctacatg aggaaaactg aaaagtcaag tacgaaattc aaattatttt ttctatattt
     1381 atttttctgt ttttttattt aacatttcaa taacagttcg aagatctttt ccttccgcaa
     1441 gaagtttcaa tttttccttt tcttcgacgg tataatttcg gacaaaactg gaatcaggta
     1501 tcaaaacaac gcctaatcct gctcgtgaag cagctgttgc tcctactaaa gaatcttcca
     1561 ctgcaaaggc tgaactagct tctaatcctg ttcgttccaa agctgtcaga taaggaatcg
     1621 gtgaaggctt gttctccgac acttcgtctc cgaatgtaaa cgtgtccaag taatcagcta
     1681 atttaaagtg atccaagata tctgttgctc ttttcgtaac agtggtcgta gcaatgccta
     1741 gtttcatatg acgttctttt aagtctgcca atatttcttt tgcatatggt tttaagtgga
     1801 tggctccgct tgtcagctgt tgataaaaat attcttcacg cttcgctcgg acttcctgta
     1861 ctttttcagg gctacctgct gctttcacta aaatatcaaa actttgtttc cagttttttc
     1921 cgccccaagc gtcaacgatt tcttttggga tatggatatc aaattgctga aatccccaaa
     1981 gccagccatc acgatacgtt ttttctgtgt caactaacaa accatctaaa tcaaatattg
     2041 cacctttcat ctatacatta atcctttcca aaacaataca catagtttag cacgactggt
     2101 ccaaaaagaa cagaagcaaa gaaccagcct tctcgtttat aagagttctt ggaatcgacg
     2161 gatatagatt ttcttcacag ctgtcacaag cgccagataa gcgatgactg tagcaaataa
     2221 ccataaccag taattgccag gaagtggcat caagcctaat tcagcaccaa aacttgtaaa
     2281 aggaaggaca gaaccgacaa taattcctaa tgtagtgata acaaacatgg caaatgttgc
     2341 gttgctttgg ataaatggta ctttcggtgt acgcagtgca tggagcacta aggtttgtgt
     2401 ccatagtgat tcgacaaacc atcctgcatg gaataatgcg atgaaaatca ctcgctgtga
     2461 actatccaat gtatggaaac ttccgcccac tacagcagga caaataacaa aatacatcaa
     2521 caagtaagta gtaatatcaa atatcgaact tgttggacca aagtaaacca tgaattcccc
     2581 aatactagat gcattccatt tttttggctc gtccaaatat tctttatcca tccgatccca
     2641 tggcaaggaa atgcaggaaa catcatagat cagattcaaa aacaaaagtt gtaaaggcag
     2701 cattggtaaa aatggcagga acgtacttgc aatcagcacg gaaaacatat tgccaaaatt
     2761 agaacttgct gtagccttga tatatttcat gatattccca aaagtttctc gtccagaaag
     2821 aaggcctctt tccaaaatcg tcaaatcttt ttctaaaagg atcacgtctg cagattcttt
     2881 cgcaatatcc acagctgtat ctacagaaat accgatatca gccactttca ttgcaggagc
     2941 gtcattgata ccatctccta aaaatccgac agtatgacca tttttacgta aagctgtcgt
     3001 caaacgtgct ttttgctgag gattcaattt tacgaatact tcatggtctt cagctacttg
     3061 tgctaattca tgatcactca tttccgagat tttctcaccg gtaaccaatt cattgatctc
     3121 tagacctact tgacggcaca ctgaacgtgt aactaattcg ttgtccccag tcagaacttt
     3181 tacagctaca ccatgttctt tcagagcttt caacgcttgt ttcgttgttt cttttggtgg
     3241 atcaaggaaa gcaagataac caatcaaaac catgtctgac tcatctttga ctgaaaattc
     3301 tccaacgacg cttggattgg tcttttgtgc gacaccaatc actcgtaagc catcttcatt
     3361 aagtgactgt acttttgcaa gtacggcttc ttttcgttct tttgttaatg gtgtaacagt
     3421 accgccaaga tcaataaatt tagaaatgtt cagcatttct tcaatcgcgc cttttgtgat
     3481 catttgtgtt ttaccagctg gatcagcaac cacaacactc atacgacgac gttcaaaatc
     3541 aaaaggaatt tcatccactt tttgataatt gatcttatct gtatctaaag tttctgttgc
     3601 ttcatcaatg atagccacat ctaacaaatt tttcagccct gtttgatagt agctatttag
     3661 atacgcatgg cgcaaaacac gatcatcttc atttccttca caatccatat gatattcaag
     3721 aatgatttta tcttgtgtca atgtccccgt tttatccgta cataacacat cgattgcacc
     3781 aaagttctgg atggcattca aatttttgat cactgttcct tttttggcca tcacacttgc
     3841 tcctttgacc aaattcgtcg taacgatcat tggaagcatt tcaggtgtta gaccaactgc
     3901 tacagatagt ccaaaaagaa aagcttctag ccaatcccct tttgtaagcc cattgatgac
     3961 gataactgct ggagccatca atgacataaa tttgatcagt agcatggatg ttttatggat
     4021 gcctaattca aagcttgatt gaattggttt ttcagatagc gtttctgcta catgaccaaa
     4081 tagtgtttca tttccaacag aaacaatgat tccttccgca cttccactga tgacattact
     4141 tcccataaag acaagatttt cgtaacttgt ttcgctttgc tgatcgtctt cacatacttt
     4201 cgcatgcttt tctactggat agctttcccc tgtcaatgct gcttgggaaa tgaataaatc
     4261 ctttgcggac aataaacgca tatccgctgg tatcatatcc cctgctgaaa gccgaacaag
     4321 atctccacag acgatttctt ccataggaat ctctgtgtac tttccattac gttttacagc
     4381 tgccgttact ttcacaagtg atttcagttt ttcagccgct tgattggaac gtacggactg
     4441 aatcaacgtc attgtcccgc tgacgataac catagtgaag ataatggcta caccgaaaag
     4501 atctttatct ccaggagctg ctaatacata ttccgtgatg aatgaaataa ttcctaatgc
     4561 gatcaacact aatgtaaatg gcgtgatgta tgctttcaat atttctacaa tgaatggtgt
     4621 ttttttgcca tatgaaatcg cattttcacc gtaattttct cttgaacgtt cgatttgttc
     4681 ttctgtcaat ccatttgctg atgcatggaa tagttcaaat aattcttgtt tcgtttttcc
     4741 agcaaaagct gcatagattt tttgtgtatc tttctttttt tccatctcaa tctctccttt
     4801 gtgtccaaaa gacagctgtt ctcgctgctt ttggtcttta gctattcatc ggatcgatag
     4861 agaaaaaact gaaaggggct gagaacagat agataatagc cctggccttt cctctagatc
     4921 cttgtttttc gaatgactgt catccatttg ttctcatctc ctttactaag taattttaat
     4981 tcatgacttc caatcatttt tcgaatagtt atttttccaa ttagaccacc tcctttttat
     5041 ttttcaccga tggggatttg cggcaacaaa aaaacctctc gccaaatact cagcgaaagg
     5101 tttgaaaaag catattaaaa aaagcttctc agatctcatc gttgagtttt gacactgtac
     5161 aacgtaaaaa gcagatacct gcttttagct accttatgaa aagccttatc tcgacaattc
     5221 ctgttctacc cattggcgtc tctcgacgtt tttgggcagt agcctatgtt tgtataggag
     5281 cctcacctaa caaatcttat tcggttttcc ttcgttaata taacaactaa aaaagaggat
     5341 gtcaaatcaa ttttccatgt ttttcatact ttttttaaaa tacacacgtt atattcctgt
     5401 ctcacgcggt tttatttgtc tcattttcca cctcccgcta taatagaaat atagtaggaa
     5461 acgaggtgct gttatggaaa gaacacttgt tattatcaag ccagacggcg tgagacgcca
     5521 tttagtcgga agcatcatcc aacgttttga agcaaaggga ctggccattg ctgaaatgaa
     5581 gtttgaaact atgactcctg aattagcaaa agaacattat cagcacttga ctgagcgcag
     5641 tttttttgat gaactgatcg attacatgac ttccggtcct gtcgtttatc ttgtgctggt
     5701 cggcgaggaa gtgatcgata tcgtccgtaa gatggttgga gcaacaaaag ctgccgatgc
     5761 tgttccaggt acgatccgag gagactacgc gctgccgggg actgaaaaca tcattcacgc
     5821 ttctgattcc cgtgatgctg cggtaaaaga gatcgcccgc tttttcccgc cattcgatat
     5881 aaacaaaccg tctgaaaaag cggcgaagtg atacaaattc taaactgctg gtctagttgc
     5941 tgggttcagc aatggccagc agttttttct ctaaggacct ccgttgattc tttcctaaca
     6001 tatattcgag cactgtgaag aattcttggt agtctttcgt cttttcttcc cctaaagcag
     6061 cagaaatcaa tctttgataa taatctgcca tcaatgtttc atagatcact gtacttcttt
     6121 ctttatgtgc taagctccgt ttcatatctt cattcacact acgtgccttt ttcaaccgac
     6181 cattttctat ataaaacgaa gtcaggttca ttaaaaaagt aaagagatac tgctcatgat
     6241 tgttcaactg tgctaatcgt cgcattcttt ttttgatttt caaataattg ttctcaatga
     6301 accgatcact gaatatccaa ggaaaatcag ctagaataga tagttcaaat tgaccatagt
     6361 ctggtatctg catcaagtat tcttcaatga ttcggcattc ataacgtaaa aattcaaatt
     6421 ctgtctcaga tacttctgtt ttttcgacca tgtgaagatt caaaaccaaa taatacagat
     6481 cttctgtttt cttaaaatgc ctacggttat tgctgatctc tttataagga tcaaagctgt
     6541 cttcttgtac ccgatctagc attcgtcttt ttttctttaa ctttattggt tcatccagct
     6601 ggtttcgcat ccattcatat tctgcaagtg tcatgttcat tcgctctaaa aatgatctca
     6661 atgttacaaa tgacattttt ttcactgaat ttgtctcaat cccgactaga gaagtcttaa
     6721 aacagattcc ttccgctaat tgggactgtg tcaacttctt gttgactcga agtttcttga
     6781 tcaattcacc atcatacata atagttctcc tttttaattt aaatttttcc acattcaata
     6841 aatagatacg ttaaaaagga aaaatacacg aaaaaaataa aaaaatgttt ttttcataaa
     6901 aaataccaac cttcaagcat tagaatgttg tctaactact gaaggttggt ttgattgttt
     6961 attgattatt tttttgctgt tcaacgtctg ttcttgttac tggatataag ttggttgttg
     7021 ttttattacc attttttgaa aagacacttg tcgaatgttc gcctttttgc ttttcgattg
     7081 cttcattacg ttccgcttca tttttatagt catatttttt cggatcaatc gtctcaaatc
     7141 cgtttggcac ataaaaccgc aagagatttt cctgattcag cttatctgaa agacttaacg
     7201 cagtcttgac tttttcctga tctttggaga tttcctcatc gattcctgct gctttcgtat
     7261 cgatgatttc tcctgtctga ttattgtaag cttttcctcc taggacggtg tatttgggtg
     7321 tcacaaagtt cccatttcga aaagcaacta cttgatcatg ctgagaagat aataagtctg
     7381 taccaaaatg aagataatct ttatcatcga ttcccaatag atgtagtaat gtcggcaaaa
     7441 catcgatttc tccaccgtat tcatgattga ctgtcccttt agtataacca ggcatatgga
     7501 tcatcaatgg cactcgttgc atctgtgcgt tgtcaaattc atcccatgta tctggatctt
     7561 taccaagagc gcttgctaaa tctttattat ctgtattcga gatcccaaaa tgatccccgt
     7621 aaatcacaaa gattgaattt tggtaaatac cagatttttt caaata
//
LOCUS       DACEEZ010000106         7622 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20313_2017
            SAMN12639966-rid9879223.denovo.106, whole genome shotgun sequence.
ACCESSION   DACEEZ010000106 DACEEZ010000000
VERSION     DACEEZ010000106.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639966
            Sequence Read Archive: SRR10023158
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7622)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7622)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741683.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741683.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:44:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,016
            CDSs (total)                      :: 2,962
            Genes (coding)                    :: 2,889
            CDSs (with protein)               :: 2,889
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 36 of 73
            Pseudo Genes (incomplete)         :: 29 of 73
            Pseudo Genes (internal stop)      :: 18 of 73
            Pseudo Genes (multiple problems)  :: 8 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7622
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639966-rid9879223.denovo.106"
                     /isolate="E20313_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(105..629)
                     /locus_tag="IWO89_002527"
     CDS             complement(105..629)
                     /locus_tag="IWO89_002527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAQ0457233.1"
                     /translation="MSEEVGIILREAVPGDAKDILLMMGQVNKETEFLVLDEAELLLP
                     PETLEEELDYIYESNNNLLLLAIYEGTIIGTASVKADSQFRLSHVGEVGISILQEYWG
                     MGLGTLMLEEIISWAKEMGVLFRLELDVQVRNERAVHLYRKMGFQIEAVMPRGARTDL
                     GEFLDVYKMSYLIE"
     gene            complement(622..1095)
                     /gene="tsaE"
                     /locus_tag="IWO89_002528"
     CDS             complement(622..1095)
                     /gene="tsaE"
                     /locus_tag="IWO89_002528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HAQ0457234.1"
                     /translation="MIELSGLTMTEQFAEAIGHSAMPGDNLILTGDLGAGKTTLTKGI
                     AQGLGITQMIKSPTYTIIREYSQGRIPLYHMDIYRVAASGADLGLDEYFEGDGLSVVE
                     WGNLLEEALPEDYLELILEKSDTDLEKRYVKLISYGEQSETFKQRILEKWAENHE"
     gene            complement(1218..2198)
                     /gene="pta"
                     /locus_tag="IWO89_002529"
     CDS             complement(1218..2198)
                     /gene="pta"
                     /locus_tag="IWO89_002529"
                     /EC_number="2.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acetyltransferase"
                     /protein_id="HAQ0457235.1"
                     /translation="MELFDSLRFKIVRRGIKIVFPEATDARILGAAARLKAEELVEPI
                     LVGNEEEIRNAAHARGIKTSSFTIISPDNYDKWDEMVDTFVERRNGKASKEQAEKILK
                     DVNYFGTMLTYMGIADGMVSGAIHSTGDTVRPALQIIKTKPGISRTSGAMIMVRGRDQ
                     EKYVFSDCAINVNPTAQELAEIAVDSAKTAELFDIEPKVAMMSFSTKGSAKSPEVDKV
                     VEATKIAKKLAPQYMIDGELQFDASYVPSVAQLKAPDSEVAGQATVFVFPELQSGNIG
                     YKIAQRFGNFEAIGPILQGLNKPVSDLSRGANEEDVYKLSIITAAQTLID"
     gene            complement(2351..3031)
                     /locus_tag="IWO89_002530"
     CDS             complement(2351..3031)
                     /locus_tag="IWO89_002530"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HAQ0457236.1"
                     /translation="MKEIIHNSWQEVLSSEFSKDYYLHLREFLKKEYASQKIHPDMYH
                     IYEALELTPYEEVKVVILGQDPYHGENQAHGLSFSVQPGVKIPPSLRNIYKELYDDLG
                     IAPVQHGNLVSWAKQGVLLLNTVLTVREGQAYSHRGKGWERLTDTIIEKLNEREKPIV
                     FILWGKPAQEKIKMIDKSRHIIITSPHPSPLSASRGFFGSKPFSKTNDALLALGEEPI
                     DWQLPETV"
     gene            3157..4026
                     /locus_tag="IWO89_002531"
     CDS             3157..4026
                     /locus_tag="IWO89_002531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HAQ0457237.1"
                     /translation="MIKLIASDMDGTLLDAHMSVSPENAEAIRFANDAGVEFMVATGR
                     NYQEARAALDEVGIDCAMITLNGAQVFDKEGNSLFTVSIPNNQAISVLDILDANGIYY
                     EVATNDGLYSENQAKRIESFASMVATHLPHLTYKMAIAMASAKLELLHITYVDSIRAI
                     LEEKNLEVLKIICFHTEGPAVLGPVGKQISLLGDLAVTSSGQNNIEVNHKNAQKGIAV
                     AHVAHERGISLEEVMTIGDNFNDVSMLQVAGVSFAMGNAEIEVKDYAKYTTDTNLESG
                     VGKAILRAIAEDL"
     gene            4052..4567
                     /locus_tag="IWO89_002532"
     CDS             4052..4567
                     /locus_tag="IWO89_002532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0457238.1"
                     /translation="MSEFFIQEKQLGRATRTIVKNEKGHSLFLMVGRWGTKGDALSLY
                     AMNGDLVAHIKQISLTFGTRFELYKEFEKVGTMQKIFNWPGDFYYIRRLHWTVQGDIY
                     NHQYQIHHFNQQIMKMDKATLFSGDYYVLDVSNDEDAPVCICIAAVLDYWLYRKDKSR
                     DKPVSYRLNLS"
     gene            complement(4614..5177)
                     /locus_tag="IWO89_002533"
     CDS             complement(4614..5177)
                     /locus_tag="IWO89_002533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAQ0457239.1"
                     /translation="MIRFARKEDAPEIAPLILVILKDMELPFLKQTGEQKTLEILEEA
                     ITDPNYRYSYSRGIVEEIDGKVAGVAYGYTDEEESIIDQPLVPILEKYQLDASTRLFI
                     DKETFPNEWYLDSISVSEDFRGQGIGSRLLEALPKLAKKANRSVIGLSVDEKNPKAKK
                     LYERHDFKVVGQRMISGHLYDHMQKNI"
     gene            complement(5232..5411)
                     /locus_tag="IWO89_002534"
     CDS             complement(5232..5411)
                     /locus_tag="IWO89_002534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0457240.1"
                     /translation="MEKMKCPNCGKKFAYEEVNNVVEHNDKEMPVVCPYCRTEAARIV
                     THGYFITEKIEDFLK"
     gene            complement(5488..5997)
                     /locus_tag="IWO89_002535"
     CDS             complement(5488..5997)
                     /locus_tag="IWO89_002535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0457241.1"
                     /translation="MKKMMFGVAALIVIGITGMTGLLNAAFHMDDISDQEYRISLKKA
                     AAIVKNETKEHNISAIHFAVEKDLENPTNATYEYQFFCEGQVIAVNPSTGKTTVRNDT
                     EKAAEHLFDIEKTEEFKQPQAAMKEAVSYYGKKAKVKEWTLLVKDERPYYKVRLIDGE
                     QDRQVWISA"
     gene            complement(6093..6473)
                     /locus_tag="IWO89_002536"
     CDS             complement(6093..6473)
                     /locus_tag="IWO89_002536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HAQ0457242.1"
                     /translation="MNLTTIHHIAIIVSDYQKSRDFYVNKLGFEVIRENYRKERDDYK
                     LDLKLGSAELEIFGIANAPQRPNYPEACGLRHLAFKVDDIEETIAELQSLGIESEPIR
                     EDTFTGKKMTFFFDPDGLPLELHE"
     gene            6803..7552
                     /locus_tag="IWO89_002537"
     CDS             6803..7552
                     /locus_tag="IWO89_002537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide mononucleotide transporter"
                     /protein_id="HAQ0457243.1"
                     /translation="MKSYFQFLAHQLKGWPQQNYYLFFFSLGCQVMTLVNSPITWLSV
                     ITFIGTTLGVLCILSINAAKSVNGILGILSAICFIIVGFSAKNYLSIGEQIAYMITLD
                     IPVLLSKEWNHNMASKIRKFTGKSWFIAIISTFLVYLVSGFLIGRYTDDPRPWVDAIA
                     FSISLTAGIISFLRYNNQYYWWLASGLAQMVLWYISFTHGSASLAMFVNSSVYLLNDV
                     LAFTVSPWYNQKERERMQKMEEEYYNAQQHA"
BASE COUNT         2168 a         1580 c         1354 g         2520 t
ORIGIN      
        1 tattcaaaaa aaatggactg cgacacgact tttgtcacag tcccttcgtg tgtttttctg
       61 ccttctcaaa aggatgacag tttctcactc ttttcctatt tcttctattc aatcaaataa
      121 ctcattttat atacatctaa aaattcacct agatctgttc ttgcccctct aggcatgacc
      181 gcttcgattt ggaagcccat ttttcggtaa aggtgtacgg ctctttcatt acgaacttga
      241 acgtcaagct ccaatcgaaa aagtacaccc atttccttcg cccaactgat gatttcttct
      301 agcataagag tgcctagtcc cattccccaa tattcctgca gaatactgat accgacttct
      361 cccacgtgag acaaacgaaa ttgactgtct gccttgacag atgctgttcc aataatcgtc
      421 ccttcataga ttgcaagcag caataaatta ttattgcttt cgtatatata atccaactct
      481 tcttctaagg tttctggtgg taacaggagt tctgcttcat ccaaaactaa aaattctgtc
      541 tccttattaa cttgtcccat catcaaaaga atgtcttttg catctcctgg cactgcttcc
      601 cgcaagatga tcccaacttc ttcactcatg attttcagcc catttttcta gaattcgctg
      661 tttgaaggtt tcactttgct ccccgtatga gattagtttc acataccgct tttctaaatc
      721 tgtatcactt ttttctaaaa tcagttccaa ataatcctcc ggcagtgctt cttctaatag
      781 atttccccat tcaacaacgg aaagcccatc accttcaaaa tattcatcta accctaaatc
      841 tgctccgcta gctgctaccc gataaatatc catatgatac aacggaatcc gaccttgact
      901 atattctcta ataatcgtgt atgtcggact tttgatcatt tgcgttatcc ctaatccttg
      961 ggcaattcct ttcgtcaacg ttgttttacc cgcacctaga tcaccagtca atatcagatt
     1021 gtcacctggc attgcggaat gaccaattgc ctctgcgaat tgttctgtca ttgttaatcc
     1081 tgatagttcg atcatttttc atcttctccc gctcttcttt ctttttgcca gtatatcgcg
     1141 taatgaagtt tttttaaaga aaaaatgaac tagtcaagaa cagcagctgc tgtttttggc
     1201 tagttcgcgt gtatttgtta atcaatcaat gtttgtgctg ctgtgataat cgataattta
     1261 tatacatctt cttcatttgc tccacgagac aaatcagaaa ccggtttgtt caagccttgt
     1321 agaattggtc cgattgcttc aaagttaccg aaacgctgag caattttata gccgatattt
     1381 cctgactgca attctggaaa tacaaagact gttgcttgac cagctacttc tgaatctggc
     1441 gctttcagtt gtgcaactga aggaacataa gaagcatcaa attgcaattc gccgtcgatc
     1501 atgtattgtg gcgctagttt tttagcgatt ttagttgctt caactacttt gtctacttct
     1561 ggcgatttcg ctgagccttt tgtcgagaag ctcatcattg ctacttttgg ttcgatatcg
     1621 aacaattctg ctgtttttgc agaatctacg gcgatttcag ccaattcttg cgcagttgga
     1681 ttcacgttga tcgcacaatc cgaaaagaca tatttttctt gatctcttcc acgtaccatg
     1741 atcatcgctc cgcttgtccg actgatgcca ggctttgttt tgatgatctg aagtgctggg
     1801 cgaacagtgt cgccagtcga atggatcgct ccagagacca ttccgtctgc aatccccata
     1861 tacgtaagca tcgtaccaaa gtaattcaca tcttttaaaa tcttttctgc ttgttctttg
     1921 gatgcctttc catttctacg ttcaacaaaa gtatccacca tttcatccca cttatcgtaa
     1981 ttatcagggc tgatgatcgt aaagctagat gttttgattc ctctggcatg cgctgcattt
     2041 cttatttctt cttcgttccc tactaaaatg ggttccacaa gttcttctgc tttcaaccgg
     2101 gcagctgcgc ctaaaatacg agcatcagta gcttccggga aaacgatttt gatcccacgg
     2161 cgaacaattt taaatcttaa actatcaaac aattccacaa gatgacctcc agacgttttt
     2221 atatgtatta tcatacacta ttccagaaaa aaagaacagt ttattttatc tgtatcagtc
     2281 aaaaatgctg gaaaaattaa gtttttcaca tacaaagcga actgcactat aacaggaaag
     2341 cgctgtttta ttacactgtt tcaggcagtt gccaatcaat cggttcttct cctaacgcaa
     2401 ggagcgcatc attcgttttc gagaagggtt tggaaccaaa aaagccacga cttgcagata
     2461 acggactggg atgtggagat gttatgatga tatggcgact cttatcgatc attttgattt
     2521 tttcttgtgc cggttttccc cacaagataa acacgattgg tttttcccgt tcatttaatt
     2581 tttcaatgat cgtgtctgtt aatctttccc aacctttccc gcgatgagaa tacgcttgcc
     2641 cttcccgaac ggtaagaacg gtgttgagta aaaggactcc ctgtttagcc catgacacta
     2701 gatttccatg ttgaacaggt gcgattccaa gatcatcata aagctcttta tagatatttc
     2761 ttaacgatgg cgggatcttt acgccaggtt gcacagaaaa agataagcca tgagcttgat
     2821 tttctccatg ataaggatct tgtcctaaaa tcaccacttt aacttcttca taaggagtca
     2881 attccaatgc ttcatagata tgatacatat ctggatggat cttttgggac gcatattctt
     2941 tttttaaaaa ttctcttaga tgcagataat aatcttttga aaattctgag gacaaaactt
     3001 cttgccaact gttatgaatg atttctttca aaaactccac ctcctaataa aaagaataac
     3061 tcacattttc ttcaatcaca agggacaatc ttcaccctca gcacttgatt aatatggtaa
     3121 actaggaaca agaagcagaa aggaacgata atcattatga tcaaattaat tgcatcagat
     3181 atggacggca cattattaga tgcccatatg agtgtttctc ctgaaaatgc cgaggcgata
     3241 cgttttgcaa atgatgccgg tgttgagttc atggtcgcta ctggtcgaaa ctatcaagaa
     3301 gctcgcgcag ctttagatga ggtaggaatc gattgtgcga tgatcacttt gaacggggca
     3361 caggtatttg ataaagaagg aaattcattg tttaccgtgt cgattccaaa taatcaagca
     3421 atctctgttt tagatattct agatgctaac ggtatttact atgaagtagc aaccaacgat
     3481 ggtctttatt ctgaaaatca agcgaaacga attgaaagtt ttgcttccat ggtggctacc
     3541 catttacctc accttacgta taaaatggcg attgccatgg cctcagctaa gctggagtta
     3601 ttgcacatca catacgtgga cagtattcgt gctattttgg aagaaaagaa tctggaagtt
     3661 ttaaaaatta tttgttttca tacagaaggg ccagctgtat taggtccagt tggaaagcag
     3721 atttctcttt taggcgatct agccgttact tcctctggtc aaaacaatat cgaagtgaac
     3781 cataaaaacg ctcaaaaagg aattgctgtt gcccatgtcg ctcacgagcg tgggatttct
     3841 ttagaagaag tcatgacgat tggcgataac ttcaatgatg tcagcatgct gcaagtagca
     3901 ggagtcagtt tcgcaatggg aaatgcagaa atcgaagtaa aagattatgc caaatataca
     3961 acggacacaa accttgaatc tggcgtagga aaagccatat tgcgcgcgat tgcagaagac
     4021 ttgtagtttt atctagaaaa gagggatata gatgtcagaa tttttcattc aggaaaagca
     4081 gcttggacgt gcaactcgga caattgtcaa aaacgaaaaa ggacattccc tgtttttgat
     4141 ggttggtcgc tggggaacaa agggcgatgc attgtctctt tatgccatga atggcgatct
     4201 cgttgctcac atcaagcaga taagcctgac attcggtacg cgttttgagc tgtataaaga
     4261 gttcgaaaag gtgggaacca tgcagaaaat attcaattgg cccggtgact tttattatat
     4321 tcgtagactt cactggacag tccaaggtga tatctataat catcaatatc aaatccatca
     4381 tttcaatcag caaatcatga aaatggataa agcaacactt ttttcaggag attactatgt
     4441 attggatgtc tcaaatgatg aagatgctcc agtttgtatt tgtattgctg cggttttaga
     4501 ttactggctc taccgtaaag acaaaagtcg cgataagcct gtttcttacc gacttaactt
     4561 aagttaaaaa gatgtacctg aaatgcggaa atacgcattt caggtacatc tttttagata
     4621 ttcttttgca tatgatcata aagatgacca ctgatcatcc gctgaccgac aactttgaag
     4681 tcatggcgtt cgtataattt tttagccttt ggattttttt catccacact tagaccgatg
     4741 acggagcgat tagctttctt cgccaattta ggtaatgctt ctaataatcg cgaaccgatg
     4801 ccttgtcctc taaaatcttc tgatacactg atagagtcca aataccattc gttgggaaac
     4861 gtttctttat caatgaacaa acgtgtagac gcatctagct gatatttctc taagataggt
     4921 accagtggct gatcgatgat tgattcttct tcatctgtat aaccgtaagc aactcctgca
     4981 actttaccgt ctatttcttc tacgatacct cgtgaataac tgtaacgata atttggatca
     5041 gtgattgctt cttctaaaat ctctaaagtc ttttgttccc ctgtctgctt caaaaatggc
     5101 agttccatat cttttagaat cactaagatc aatggggcga tttctggagc atcttctttt
     5161 ctcgcaaaac gaatcatagt ttcctctcct tttctccatt ctaacatgca gaaaaagaaa
     5221 tcgcaaaatc gttattttaa aaaatcttca atcttttcag taatgaaata accatgagta
     5281 acgattcttg ccgcttcagt tcggcaataa gggcagacga ctggcatttc tttatcatta
     5341 tgttcgacta cattattgac ttcttcgtat gcaaattttt ttccgcaatt agggcatttc
     5401 attttttcca tgttatctca acttcctttc atctttactc tcttagctta ctcttatatt
     5461 gtaaaaaaga gaagcaatat ccttcttcta tgcagatatc catacctgac ggtcctgttc
     5521 tccatctatt aatcggactt tataataagg acgttcatct ttgactagaa gtgtccactc
     5581 tttcactttt gcttttttcc cgtagtagct gactgcttcc ttcatcgctg cttgcggttg
     5641 tttgaactct tccgtttttt ctatatcaaa aagatgttct gcggcctttt ccgtgtcatt
     5701 tcgtacggtt gttttcccag tggatggatt gactgctatg acttgtcctt cacaaaaaaa
     5761 ctggtattca tatgttgcat tcgtaggatt ctccaaatct ttttcaactg caaaatgaat
     5821 agcagatatg ttatgttctt ttgtttcgtt ttttacgatt gctgctgctt ttttcaatga
     5881 gatccgatat tcctggtcac taatatcatc catgtgaaaa gcggcattga gcagtcccgt
     5941 cataccggta atccctatta cgatgagggc agcaacacca aacatcattt ttttcattct
     6001 tcattctcct gtttcttttt tttaaagaat atcaagaaat gaacgaatga aaaatcgaat
     6061 gaaacgaaaa aaaagtgaca attttgttat ctttattcgt gcagttctaa tggcaatccg
     6121 tctggatcaa agaaaaacgt catttttttt cctgtaaagg tatcttctct aatcggttct
     6181 gattcgatcc ctaaggactg caattctgca attgtttctt caatatcgtc tactttgaaa
     6241 gcaagatgac gcaagccaca agcttctgga tagtttggtc gttgtggcgc attagcaatt
     6301 ccaaaaatct ccaattcagc acttcctagt ttcagatcta atttatagtc atctcgttct
     6361 ttgcggtaat tttctctgat tacttcaaat cctaatttat taacataaaa atcacgtgat
     6421 ttctgataat ctgaaacaat gatggctata tgatggatcg ttgtaaggtt catatgagca
     6481 ctcctattct tcgtttaagc tcggatctat attaatctcc agttccattg taaatgtgtc
     6541 agattgaaaa aaatagctgt ttttcctttt tataagaaaa aaggcaaaaa aaacagaaag
     6601 taggtcctcc aagacactct tcctgttccc aatataataa atgaaagtaa gaaattatat
     6661 tttcaagaaa acaaacagaa aattatcata acacaaatta tttataattg aaagactagt
     6721 tttttttcaa gaaaagcata caatagattg tgttagtaaa tacagaacac actacattta
     6781 gtgtcttaag gagtcctcat acatgaagtc atattttcag tttttagctc atcagctaaa
     6841 aggctggccg caacaaaatt attatctttt ctttttcagt cttggctgcc aggtcatgac
     6901 tcttgtcaat agtcccatca cttggctgtc cgttattaca tttatcggta ccacgcttgg
     6961 tgtattgtgt attttatcca tcaatgcggc aaaatcagtc aacggtattt taggtatttt
     7021 gtctgcgatt tgttttatta tcgttgggtt ttcagcaaag aactatttaa gtatcggtga
     7081 acagattgct tatatgatca cattggatat ccccgtattg ttaagtaaag aatggaatca
     7141 taatatggct tcaaaaatcc gaaaattcac tggaaaaagc tggtttatcg caatcatcag
     7201 tactttcctt gtctatcttg tttcaggatt cctgattggt cgttatacag atgatcctcg
     7261 gccgtgggta gatgccattg ctttttctat tagcttgact gctggtatca tcagtttctt
     7321 gcgttacaac aaccaatatt actggtggtt agcttcaggg cttgctcaaa tggtcctttg
     7381 gtatatttca tttacgcatg gatctgcaag tttggcgatg ttcgtgaaca gctctgttta
     7441 cttgctgaat gatgtccttg catttaccgt ttcaccttgg tataaccaaa aagaacgtga
     7501 acgtatgcaa aaaatggaag aagaatacta caacgcacaa caacacgcat aaaaaaactg
     7561 agcgtgggac aaaagtaaag aacacttttg tctcacgctc taaacacgta taaacggcgg
     7621 aa
//
LOCUS       DACEEZ010000107         7612 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20313_2017
            SAMN12639966-rid9879223.denovo.107, whole genome shotgun sequence.
ACCESSION   DACEEZ010000107 DACEEZ010000000
VERSION     DACEEZ010000107.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639966
            Sequence Read Archive: SRR10023158
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7612)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7612)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741683.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741683.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:44:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,016
            CDSs (total)                      :: 2,962
            Genes (coding)                    :: 2,889
            CDSs (with protein)               :: 2,889
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 36 of 73
            Pseudo Genes (incomplete)         :: 29 of 73
            Pseudo Genes (internal stop)      :: 18 of 73
            Pseudo Genes (multiple problems)  :: 8 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7612
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639966-rid9879223.denovo.107"
                     /isolate="E20313_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(15..707)
                     /locus_tag="IWO89_002538"
     CDS             complement(15..707)
                     /locus_tag="IWO89_002538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HAQ0457244.1"
                     /translation="MQIELQHTHDKETFEKNYKVYFELARDSAMKYGIPLTLDTPYNQ
                     PGIKSHLWVTQNIWGDHTDPYGYLSEMGVSKEKLAYDLAHGFTDDNPTTSEDKPVIDP
                     TRAGAANSTLTDGTNYAHIDQFGEIENANLHVAGWHIANYKYEYIFIMDYNTGKELAR
                     VRADGIYRPDVNQAYNTLGNVGYHVSFNMRDFPNKKVYVMMRATNDPEGNTKGGAQDF
                     HDKRWYLNIPQR"
     gene            792..968
                     /locus_tag="IWO89_002539"
                     /pseudo
     CDS             792..968
                     /locus_tag="IWO89_002539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348541.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase"
     gene            complement(1144..1713)
                     /locus_tag="IWO89_002540"
     CDS             complement(1144..1713)
                     /locus_tag="IWO89_002540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HAQ0457245.1"
                     /translation="MELKKLEVPTSSITEVKRSPMDVFAQARDAGTGVYIFNREKVAG
                     VMLTQEQYETLLQELDVLRQTVKVERKEPKEINEEPINKTASVVSNAEGMEELAQTLQ
                     HSLITGVTITAKNLDERMVALGFITKKTGFGGVVDMLSELMETGKINYQLRRKPQNRT
                     IIAEIIGEQNSQSQLMDKIIIKKIFLHEL"
     gene            complement(1787..2110)
                     /locus_tag="IWO89_002541"
     CDS             complement(1787..2110)
                     /locus_tag="IWO89_002541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0457246.1"
                     /translation="MLLLKTLQERGYLDVHPPEVKIVFFFRYEHQEARRLAGVLNKTL
                     RHRKRISIHVCTRNKPPRYADLVIIDHFFPLDHNEDQKTYLYHPSFGIERLLTDINYW
                     LGKNR"
     gene            complement(2264..3076)
                     /locus_tag="IWO89_002542"
     CDS             complement(2264..3076)
                     /locus_tag="IWO89_002542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008274430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0457247.1"
                     /translation="MRWQKLLSKNAQNLFSLINLMKDLIWRKADLAEELKIDVKTVDR
                     HLKRLAESDCPIQLILHKKTVQLVYDQGYSEPYLLFVFLIQSPPFCLLKNLLLNEPYE
                     QKYDRSTQERLRKWFGEYHLSFSYKKAQIFGSERKVRYLLFCFLKDFPHFFADDGEQS
                     FSEMFIQLLKQRKDPAEIEILGDSRTLFFELFPELLFREEQILPIKEKNYLAILRLSC
                     NQITEKRWKRIEETQDYRKLTIVTEKLDSLFWLNSEFESESKSKLTKVLYQE"
     gene            3430..4278
                     /locus_tag="IWO89_002543"
     CDS             3430..4278
                     /locus_tag="IWO89_002543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008274428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HAQ0457248.1"
                     /translation="MIVALLAGGHVLFEDIPGVGKTLLVKALAKAVQGTFSRVQCTPD
                     LLPSDILGFSVYNSQSKEFEFRPGPIFTTILLADEINRTTPRTQSALLEAMAENHATI
                     DNNTYPLDNHFFVLATQNPIEYEGTYPLPEAQLDRFLFRLQIGYPSFDDELLLLLDKF
                     EKVLDNLNEVLNSYEIEEIKTNVANVFVSPEVASYALQLVSATRSHAAIQLGISPRGS
                     LSFIQAAKAYALIHGRNYVTPKDLQDLVPYVFSHRLIFYDRSLKEDEKKQIIHTLVAQ
                     IPIPVR"
     gene            4423..5364
                     /locus_tag="IWO89_002544"
     CDS             4423..5364
                     /locus_tag="IWO89_002544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HAQ0457249.1"
                     /translation="MELDFIKQLSKETFGYLYIAATRLSQKKYAGYFFHSKKENFFFT
                     SPSFTNRLPSNLCRKKQEILILTNKPQEVQLLWQPKERGCHETLTVIFTAYDSLGLFR
                     KSAKREISFPVTVLPAFCKIQAEKLQRVLEKKQQLNAYQRGLDFREHRAYRPGDSFNR
                     IDWKLSAHTQEWLYREYDYQEEECSPLFCFWGLPSPKFEPLLDLYFSLWHLRKEKQPE
                     LLILGNRAFHGKDPGHTYFASLEAGDEKAFFAHLKKYAKKQIVLFVPENSPEVSEAIE
                     TLSKDRTVYLVYFVEKQLMVQEKDKICSLPGGEWIDD"
     gene            5354..7525
                     /locus_tag="IWO89_002545"
                     /pseudo
     CDS             5354..7525
                     /locus_tag="IWO89_002545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343611.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase domain-containing protein"
BASE COUNT         2269 a         1505 c         1372 g         2466 t
ORIGIN      
        1 gaggggggga ttttttatcg ttgtggaata tttaaatacc aacgcttgtc atgaaaatct
       61 tgtgctccac ccttagtatt tccttctgga tcgtttgtcg cacgcatcat gacgtatact
      121 ttcttattag ggaaatcacg catattgaaa gatacatgat agccaacatt tcctaaagta
      181 ttataagctt gatttacgtc tggtctataa atcccatcag ctcttactct agctagttct
      241 tttccagtat tgtagtccat aatgaaaata tactcgtatt tataattagc aatgtgccat
      301 ccagctacat gcaagtttgc gttttcgatt tctccgaact gatcaatgtg ggcgtaattt
      361 gttccatctg tcagcgtaga atttgcagca cctgctcgag ttggatcaat tactggttta
      421 tcctccgaag tagttggatt atcatccgta aaaccatgag ccaaatcata ggctagtttt
      481 tctttactta cacccatttc agaaagataa ccgtaaggat ctgtatgatc gccccaaata
      541 ttttgtgtta cccataaatg cgatttgatt cctggttggt tataaggagt gtctaatgtt
      601 aatggaatac catatttcat tgctgaatct cttgccaatt caaagtatac tttatagttc
      661 ttttcaaaag tttctttatc atgtgtatgt tgtaactcga tttgcacagg acttttggct
      721 cacgaggttg tcctactcta catcttgctg atagatatgc tgaaaggaaa acaccaacat
      781 tatgttttgc tttttcagct agaacgagac aagcaggaat acctttagaa gatatcaaag
      841 gttttttagg tcataaagat gtttccacta cacaagtcta tgcccatatt tctcccgaag
      901 tgaaaaaaag atcaatgaat caactagaaa actatataga agagcaaata aaaaagcact
      961 caaattgagt gcttttttaa gctcactatc actaaaacta tcactaattt tatttataag
     1021 cgaacatgtt tctatcactg attttttaaa tctagcgaaa aacccttatc ttaaaggcgt
     1081 tcgttcaatt ctttggcaag gtcttcaaag cctggcttgc ctagaagtgc gaacatggtt
     1141 ttcttatagc tcatgtagaa aaattttctt gatgatgatt ttatccatca attgagattg
     1201 actgttttgc tctcctatga tttcagctat gattgtccga ttttgcggtt ttcttcttaa
     1261 ctggtagttg attttcccag tttccatcaa ctcgctaagc atatccacga ctccgccaaa
     1321 tccggtcttt ttcgtaataa aacctagtgc taccattcgt tcatctagat tttttgctgt
     1381 aatcgtcaca ccagtaatga gcgagtgctg taatgtttgg gccagctctt ccatcccttc
     1441 agcattggat acgacggaag ctgttttgtt aataggttct tcgtttattt cttttggttc
     1501 tttcctctcc actttcactg tttgacgaag gacatctagt tcttgcaaga gcgtttcgta
     1561 ttgttcttgg gtcagcatca cgccagctac tttttcacga ttaaatatat atacgccagt
     1621 tcccgcgtct cgcgcttgag caaatacatc catcggcgat cgtttcactt ctgtgatcga
     1681 agatgtggga acttctaatt tctttaattc caatggattt cctcctttac taatcgcttt
     1741 ttattatagc acagactgac ttgttttgat cttttttcca aaggttttat cggtttttgc
     1801 ctaaccagta attgatatcg gtaagaagtc tttcgatacc aaatgaagga tggtacagat
     1861 acgttttttg atcttcgtta tggtctagtg gaaaaaaatg atcgatgatc actagatctg
     1921 cataccgagg cggcttattt cgagtacata catgtataga gatccgtttt cgatgtctta
     1981 gcgttttgtt cagtacccca gcaagtcttc ttgcttcttg gtgttcatag cgaaaaaaaa
     2041 agacgatttt cacttcagga gggtggacat ctaaataccc tcgttcctgg agtgttttca
     2101 gtaaaagcat cccccactct actgcggggt gtgcaagtgc ttgttggatc gctagccgaa
     2161 attgatatag tttatctttg gaattgggaa atttttctaa tgtttcctga taataacgaa
     2221 cttcttctat cagccggtca tggataccaa ttacgtaacg aagtcattct tggtaaagta
     2281 cttttgtcag ctttgatttg ctttcgctct caaattcgct attcagccaa aacagcgaat
     2341 caagcttttc agtcacaatc gtcaatttcc gatagtcttg tgtctcttct atccgtttcc
     2401 accgcttttc agtaatctgg ttacagctca agcgcaaaat tgctagataa tttttttctt
     2461 ttattggcaa aatctgctcc tctctaaata gcaattcagg aaacaattca aagaatagtg
     2521 ttcgactgtc tcctaaaatc tcgatttctg ccggatcctt tctttgcttc aataattgga
     2581 tgaacatctc agaaaaggac tgctctccat catctgcaaa aaaatgcgga aaatctttca
     2641 aaaagcaaaa cagcagatat cttacttttc tttctgaacc aaagatctgt gcttttttat
     2701 aagaaaaaga aaggtggtac tcgccaaacc actttctcaa tcgctcttgc gtagaacgat
     2761 cgtacttctg ctcgtaaggt tcattcaata gcagattttt cagtaaacaa aacgggggtg
     2821 attggatcag aaaaacaaac aagagataag gttcgctata tccttgatca taaactaact
     2881 gaacggtttt cttatgaaga atcagctgaa tgggacagtc gctttccgcc aaacgtttca
     2941 gatgccgatc cactgtttta acatcgattt tcagttcttc agctaaatca gcttttcgcc
     3001 agatcaaatc tttcattaga ttgattagtg aaaataaatt ctgtgcattt tttgataata
     3061 gtttttgcca tctcatcgat catttctcac ctgatttcta aacatattgt ttgatcaaaa
     3121 tgggttcact cctaaaaaga taaaaaactc cctttctatt tatttaaata cgcaaatctt
     3181 cctcattatt ttttatttta ctcaatttaa tcgtattctc atttcaaaac gaatgtactt
     3241 taaaaaaaca taataagatc actttgcttg tatgatgtat tcccttccat tacaatgaaa
     3301 taaatagcga ttttatgaga aggtgatgtg aaaatggaag aaaaaataga agaatccaaa
     3361 caaaaaatcg atcagttgat ataagaatta gaaaaagcgg tcatcagcaa acgttcaact
     3421 atccaattga tgatcgtggc cctattagca ggtggtcatg tcttattcga agatattccc
     3481 ggtgtaggta aaacgttact ggtcaaagca ctagcaaaag ctgtacaggg aaccttcagc
     3541 agagtccaat gtactcctga tctgctgcct agtgatattc ttggtttttc cgtatacaat
     3601 tcacaatcaa aagagtttga atttcgtccg ggaccgatct ttaccacgat cttgttagca
     3661 gatgagatca atcgtacgac tcctcgtact cagtcagcac ttttagaagc aatggccgag
     3721 aatcatgcaa cgatcgacaa caatacatac cctctagaca atcatttttt tgtcttagct
     3781 acgcaaaatc caatcgagta cgaaggaaca tatcctttgc cggaagcaca gctcgatcgt
     3841 tttttattcc gactacaaat cggttatcct tcttttgatg acgaactttt gctacttttg
     3901 gataaattcg aaaaagtgtt ggataactta aatgaagtat tgaacagtta tgaaatagaa
     3961 gaaataaaaa caaatgtggc gaatgtcttt gtcagtcccg aagtggcatc ttatgccttg
     4021 cagttagtgt cagcaacaag aagtcatgct gccatccagc ttggcatcag tccacgagga
     4081 agcctttcct ttattcaggc agcaaaagct tatgctttaa tccatgggag aaattacgtc
     4141 acgcccaaag atttgcagga tttagttcct tacgtgtttt cccatcgcct gattttttac
     4201 gatcgttcat tgaaggaaga cgaaaagaaa caaatcattc atactcttgt tgcacaaatt
     4261 cctattcctg tgaggtagat tgaggtagac aatggaaaaa atcaaagatt atttcattaa
     4321 ttttgtgtta tttctggtat atttcatcat tgtgcttttt gctcttgtat tcaacaattc
     4381 tttaggctgg ttcttgttgg ccttttttac tctttatgta tcatggagct tgatttcatt
     4441 aagcagcttt ctaaagaaac tttcggttac ttgtacattg cagccactcg tttatcgcaa
     4501 aaaaagtatg caggttattt tttccatagc aaaaaagaga actttttctt tacctctccc
     4561 tcgtttacaa atcggcttcc cagcaatctt tgcagaaaaa aacaagagat tttgattctt
     4621 acaaataaac cgcaagaagt ccaattgctt tggcagccaa aagaaagagg atgccatgaa
     4681 acattaactg tcatttttac agcctatgat agtctaggac ttttcagaaa aagtgcaaaa
     4741 agagagatct cttttcctgt gactgttctg cctgcttttt gtaagattca agcagaaaaa
     4801 ttacagagag ttctggaaaa aaaacagcag ctgaacgcgt accaaagagg tcttgatttt
     4861 cgggaacatc gcgcttatcg tccaggtgat tctttcaacc gaatcgattg gaaattgtct
     4921 gctcatactc aagagtggtt ataccgtgaa tacgattatc aagaagaaga atgttctcct
     4981 cttttttgct tttggggttt gcccagtcca aaatttgaac ctctgctaga cttgtatttt
     5041 tctttatggc atttgagaaa agaaaagcag ccggaacttc tgatattagg caaccgcgcc
     5101 tttcatggca aagaccccgg tcatacctat tttgcttctt tggaagctgg cgatgagaaa
     5161 gcttttttcg cacacttgaa aaaatatgca aaaaaacaaa tcgtcctctt tgttcctgag
     5221 aattctcctg aagtctcaga agctattgaa acattgtcaa aagatcgaac ggtttatctt
     5281 gtttatttcg tagaaaaaca actcatggtc caagaaaaag acaagatatg ttcattgcca
     5341 ggaggtgaat ggattgacga ttaaacggga aaaatggctg cgtggcgttt actcctttgt
     5401 gatactaagt gtaggtgctc cgatatttgt agtagctaat caattgcaaa acagctacct
     5461 tcttatccta actattggtg tcctatgcct tattgtctgc tgtattccta aaaaatgggt
     5521 acgtttgctg ctttctttgc cagtaatgat aggttgtttg tttttatatt ttccttcgaa
     5581 gacattttcc atattatgga tcttacagtt tttttcgtca gtcacagagg aattcttcca
     5641 tctatcccaa ggtaaacttt attatttgcc agaaaagaca gctttttttc tcataatgtt
     5701 gctgatctat ttatttatta cattgaccgt ggattacgac tattggtacg caccctttct
     5761 ttctttgatg gcttacttca tgatgcttac cgtttttcgg aaaaaagatt tactgtacga
     5821 aatgattgcg gtagtaactg ttttgttcgt gtatctggca gcaaggcaat ttttctccat
     5881 ccggttgttg tcgggaaaca ctcgattcca gtcattgact acagttctgc ttatttgttt
     5941 cttaactttt agcgctatct tgcctttata tcttccgaaa gtacaccaat ctctcgagga
     6001 aacgagtgaa ccgatccgag aatatcttaa tgatgaaggg ctttatgaca cactccatga
     6061 gtatcaatta accggaagtg caagaacagg cttcagtgag aatgatgaac agcttggtgg
     6121 acctttatat gataatggcg aagttgtttt tacagcgaac caaaaaggaa atggctattg
     6181 gaagatcgaa aataaaaatc attacaccgg ttctggatgg atagaagatc aggacgcacc
     6241 catccaacca atccaagaat tccctgttga catcacgagc taccaaaccg ttatagaaga
     6301 caaagaaaac attacattga ccctaaaaaa acaagttttc ttaccttatc cttatggaaa
     6361 tactaccttg actgattttt cattagctag tggacaaacg ctttactatt tgcctgaaag
     6421 ccagcgattc attcctgtcc cgaatgaatc gacacgttct gcaactttac aaacagaaag
     6481 gttagttttt acgagagaag aattaaaaag cagtcagtta aaagctgatg atttagaaaa
     6541 tcagcagaca aatcttcaat tgccaaaaag tttgcctaaa cgtattgagg acttggcggt
     6601 cgaattgacc aaaaatgaag caactttaat tcgtaaagtc gaagctgtct aacagtattt
     6661 aaaaacaagc ggaaatttcc gctattccaa aacggatacc gggcatactc caaaagaaca
     6721 cgattatgtg gattatttcc tctttgattc aaaagtcggc tattgtgaca acttttcaac
     6781 ttctatggtc gtccttcttc gaacacttgg tatcccagct cgctggacaa aaggttttac
     6841 aaatgggctc agaacaggca cagataatca agggaatgag gaatacagca ttctcaatag
     6901 tcatgctcat tcttggccag aggtatactt tcctggattt ggctggcttc catttgaacc
     6961 aaccccttca tttgccaatc caatgggttc acaaccagtt gctgaaacaa ccgaaaccgt
     7021 ccagcctgct atcgaacaac caaatgaaag cgaaaatatg gcagaaacta tttccagtga
     7081 gagatctgag gtcattccat cagaagaaac aacactcaca ccagcttata aaatcgtctt
     7141 attcagcctt ggagtgtgtt tatccgtctt gttgttcttt ctattgttat tcagaaaatc
     7201 tctgcgctgg acaagttatc ttatctatct tcattttttt gcttctacta atacgattgc
     7261 atatcaaaaa cttcttaacc ctttggaaac attggtaccg cgcaaacccg cagaatcttt
     7321 gcaggaatat agcctgcgaa tagaacaaga attcaagcta gatctgcgtt tcattcgatt
     7381 aactactcaa tatgaagcag aaatatatgg acgacaagca gatccggtaa aattcgataa
     7441 aaaaacctat ttagaaataa cgcatataat aagaaaaatg gtatacaaaa aaattctgac
     7501 aaaagggagc cgcttcaaaa aataagataa aatttctgga gaatctactt ctggaatgcc
     7561 gagaaccagc tgattgagta tcgattactc gaaaaaaagg ttgtgacacg tt
//
LOCUS       DACEEZ010000108         7600 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20313_2017
            SAMN12639966-rid9879223.denovo.108, whole genome shotgun sequence.
ACCESSION   DACEEZ010000108 DACEEZ010000000
VERSION     DACEEZ010000108.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639966
            Sequence Read Archive: SRR10023158
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7600)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7600)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741683.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741683.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:44:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,016
            CDSs (total)                      :: 2,962
            Genes (coding)                    :: 2,889
            CDSs (with protein)               :: 2,889
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 36 of 73
            Pseudo Genes (incomplete)         :: 29 of 73
            Pseudo Genes (internal stop)      :: 18 of 73
            Pseudo Genes (multiple problems)  :: 8 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7600
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639966-rid9879223.denovo.108"
                     /isolate="E20313_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(138..428)
                     /locus_tag="IWO89_002546"
     CDS             complement(138..428)
                     /locus_tag="IWO89_002546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0457250.1"
                     /translation="MGEKKIKGAGILLVMSKALFLLPCTFMFIVLCYFKRRKKWCWID
                     VLLFLPFFPLIYFLEEIWDDMACDSAFLFSHSLGLMISLTIFAIVLFFLNKK"
     gene            complement(546..806)
                     /locus_tag="IWO89_002547"
     CDS             complement(546..806)
                     /locus_tag="IWO89_002547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0457251.1"
                     /translation="MGVDHLIVLSKLLFWIPFIIMTSAFFYLFKRPNKYLLLSACPMI
                     YFIHKIRGFYYYDSGISLFCYEIGLLASAIFFIFVLEYFRKK"
     gene            complement(943..1356)
                     /locus_tag="IWO89_002548"
     CDS             complement(943..1356)
                     /locus_tag="IWO89_002548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HAQ0457252.1"
                     /translation="MANVPSIRDMFPDFNDVFSPAFNDFLGVSSYPKVDLVENEKEYK
                     LTADMPGCDKEDTTVEYSDNTLTISANHESHTEDKEDGNYVRKERHSVSYKRSFYLPN
                     VDEEKITGTFKNGVLKLVLPKTAYQPKETKKIELN"
     gene            complement(1812..2297)
                     /locus_tag="IWO89_002549"
     CDS             complement(1812..2297)
                     /locus_tag="IWO89_002549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0457253.1"
                     /translation="MHKGIYAGLLGTVILFSACGYKTKNSVKESSAAISTTEVGSTKS
                     TSTTSITNTSTGNVDSQHSQSSIQSFSISSTSEMQESTVPSLPLENSDKALLFLIAHR
                     SELQSDDIVISFYQKIDRDYLFTASSKQIRSQGGSGSVGFYRVSPEGSITMTDANGTP
                     F"
     gene            complement(2436..2651)
                     /locus_tag="IWO89_002550"
     CDS             complement(2436..2651)
                     /locus_tag="IWO89_002550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF378 domain-containing protein"
                     /protein_id="HAQ0457254.1"
                     /translation="MKTLDAIALTLLIVGGLNWLLVGLFELDLVAMIAGGSTTIFAKI
                     IYIVVGICAIYCLKFFPMITRKVDERY"
     gene            2875..3675
                     /locus_tag="IWO89_002551"
     CDS             2875..3675
                     /locus_tag="IWO89_002551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HAQ0457255.1"
                     /translation="MSDWLDIAGKVVIVTGGSSGIGRSIVENLLKQNAQVANFDVTEC
                     RIQHENLLSLKVDVSSKTDIKEGIYKVMKHFKTIDGLVNNAGINIPSLLIDRNHPKSK
                     YELSEQVFDKMIAVNQKSVYLMSQAVGRILVQKGSGVIVNLSSESGLEGSEGQSCYAA
                     TKAAMNSFTRSWAKELGKRNVRVVGVAPGILEETGLRTQEYEEALSYTRGISVEQLRN
                     GYSRTSTIPLGRSGKLQEVADLVCYYLSERSSYITGVTTNISGGKTRG"
     gene            3694..5562
                     /locus_tag="IWO89_002552"
     CDS             3694..5562
                     /locus_tag="IWO89_002552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
                     /protein_id="HAQ0457256.1"
                     /translation="MTLVNRWYQLLKLLVTHKKVSIQEVQKEIKTSNQTIKKDIGELN
                     EEIAGIAKIIQKNKHFELEIYDFDFFDEIMQGKLKTASDFNSASKRIAYLLNRLIETE
                     DFLRMDDLSEEVGVSRGTVVKDLKTLKEMINDFDVKITGTPNKGLRIIGDELELRLLL
                     FYFVSPYFSETKTEQFLEEKQAIKHFQKKTNASKQEITLLTKVIGISLNRISSNYLLK
                     KPINRYSGCFEYIQEFNELINQLEEIYELTLSQYEKDFISFPLNIINTSITMIRKANS
                     DLRLYFDPMMMRIRRLDLIDLDESFLYQEMKQHLSLLINRVIFRYRLTDLFYGEIEKK
                     YPFSYQIASECIQALEAILQHKISKVEISYLTLYLELAFRYQKEHFSSKKVAIVHTTG
                     KGTALMIQRQIKRILGRDIQITHVSEEQYEKVDLNKYFAVFTTIPLKNVNEETPVIHL
                     TNLFDDSWLRAQWQKAKKKRLAGFHSTVFFYQIWKEKKTYQEYLNKLVAPLIRGYYVD
                     EEFEKRILKREYQQSTFVNKEFAFPHTTNQLSQHIVLSVGLITDQGLAETGGNGVIFL
                     FAIPEEMTAEVENELFELYDILFELIKMGIDEFIELAPTQESFLKLIKSKGERLHE"
     gene            5555..6046
                     /locus_tag="IWO89_002553"
     CDS             5555..6046
                     /locus_tag="IWO89_002553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR family transcriptional regulator"
                     /protein_id="HAQ0457257.1"
                     /translation="MNDMYLFIVFAASAYVLQAVISWKQLKHFNQTYSELRRKGKVAI
                     GKRPGKIRSGTIVMLAIDDHDQVLEVRKMQGVTSLAKFHSLPVYAGEDIHYIDRYHPS
                     VRAEDRLTQAALENAREVYLRIEAGNEKSEQPSLPFIAMLNNFRIWKSTIKEKRSDKR
                     WIS"
     gene            6034..6588
                     /gene="srlA"
                     /locus_tag="IWO89_002554"
     CDS             6034..6588
                     /gene="srlA"
                     /locus_tag="IWO89_002554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174078.1"
                     /note="catalyzes the phosphorylation of incoming sugar
                     substrates along with their translocation across the cell
                     membrane; the IIC domain forms the PTS system
                     translocation channel and contains the specific
                     substrate-binding site; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIC"
                     /protein_id="HAQ0457258.1"
                     /translation="MDIITKFAEGFMRLFQTGAETFISWMTGIVPVVLMLMVAMNTLI
                     AFLGEDRVTKVAKLSAKNPLTRYLVLPFLSAFMLGNPMSFTMARFLPEYYKPSYYAAQ
                     AQFCHTSNGVFPHINPGELFVWMGIAQGIETLGLNSMELAIRYLLVGLLMNFIGGWIT
                     DFTTGFVCRQQGIVLSKKVELSVD"
     gene            6606..>7600
                     /locus_tag="IWO89_002555"
     CDS             6606..>7600
                     /locus_tag="IWO89_002555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIB"
                     /protein_id="HAQ0457259.1"
                     /translation="MYGDIKIVRGNGGYGGPLIIRPTKDKHKFIYVTGGGERPMIVDK
                     IEELTGMESINGFKTSIPDSEIALAIVDCGGTLRCGIYPKKGIPTINIVPTGKSGPLA
                     QYITEDIYVSAVGLEQIQPLMETDDNKETVNTASPDKKYKYDTSKKITEQKAEQQNWI
                     AKIGMGAGKVVATFNQSAREAVDTMIRTVIPFMAFVSLLIGVIQGSGIGNVFANIMAP
                     LAGNAVGLTIIGFICSLPFLSPLLGPGAVISQVIGTLIGVEIGKGNIPPQLALPALFA
                     INTQNACDFIPVGLGLEEAKAETVEVGVPSVLYSRFLNGIPRVIIAWVASFGLYS"
BASE COUNT         2708 a         1213 c         1442 g         2237 t
ORIGIN      
        1 tttgttacta tgaataacat aataagaaag taaagtggat tgatttgaga tctttttata
       61 cttcaaaaaa gataacaccc aactttcaaa aatatgtttt ggaagttggg acaaacgagt
      121 taatacattt taatacatta ttttttattc aaaaaaaata gcacaatggc aaagatcgta
      181 agagagatca taagccccaa tgaatgacta aaaagaaaag cagaatcaca tgccatatca
      241 tcccatattt cttcaagaaa ataaataagc gggaaaaagg gaaggaatag tagaacatcg
      301 atccagcacc atttttttct tcttttgaaa taacataaaa caataaacat gaacgtgcag
      361 ggaagtaaaa acaaagcttt actcataact aacaaaatac cagctccttt tatttttttc
      421 tcccccacat tcaaagagta acaatggtat gtacttaata atagaaaaat ggatcaactt
      481 gtatgaataa cagctgaaaa gatctgcacc aaataaaaaa cttccagcca aaactgactg
      541 gaagtttatt tttttctgaa atattctaac acaaatatga aaaagatggc cgaagctaat
      601 aagccgattt cgtagcaaaa caatgagata ccagagtcgt aataataaaa tcctctaatc
      661 ttatggataa aatagatcat cggacaagca gataagagta agtatttgtt aggccttttg
      721 aataagtaaa agaaagcact ggtcattatg ataaatggta tccaaaacaa aagtttactt
      781 aagacaatca aatgatcaac tcccatattt tttaccaaga gagatcataa catctttatc
      841 gtaaaaacaa aatatttttt tgctcatttt gaaaatttaa acatactcgt tcttatccaa
      901 atatataaaa atcgcccctc attcatcgag aggcgataag ttctaattta actcaatttt
      961 ttttgtttct tttggctgat aagctgtttt aggtaatacc aatttcagca caccgttttt
     1021 aaaggttcca gtgatttttt cttcatcaac attaggaaga tagaatgaac gtttgtaaga
     1081 gaccgaatgt ctttctttcc gtacatagtt gccatcttct ttatcttctg tatgggactc
     1141 atgattggcg gagatcgtca aagtattatc tgaatattca actgtcgtgt cttctttgtc
     1201 acagccaggc atatcagcag ttagtttgta ttctttttca ttttcgacta aatctacttt
     1261 tggataactt gaaacaccta aaaaatcatt gaatgctgga ctaaacacgt cattgaaatc
     1321 agggaacatg tcgcgaatgc taggtacatt tgccattttt gatcatcctt tcaataagta
     1381 ttttttattt atccctattt acgtaataac tctattttga aaaaaatgca agtataatgg
     1441 ttagctttgt gaatatttca tctaatacga tacaaaggct atctttctaa tttgaaaaag
     1501 ggatcattaa aataataaga agaaaatatg tcaaaaggta aaaaagtaaa ttcttctatc
     1561 caaatataga aaaattgaat ctagaaaacc gcccgacaat aaatttcatt aactcagttg
     1621 accgaaaatg aagtagatac caaaaattta tcaagagagc aagtagagaa gtgagtatat
     1681 tcataagaga aatagctgta acgaattatt aggttaatgc aaatgcttat ttatactccg
     1741 tataactttc tcagaaaaaa tgataccaat aatgacttgc tagtactgta aatccgtata
     1801 atatcatcta atcaaaatgg tgttccattt gcatctgtca tcgtaatcga tccttcgggt
     1861 gacactcgat aaaaaccaac gcttccagat cctccttggg aacggatttg tttgctggaa
     1921 gctgtgaaca aataatctcg atcaattttt tgataaaagc taataacaat atcgtcactt
     1981 tgtaattcgg aacggtgggc aataagaaat agaagtgctt tgtcagagtt ttctaatgga
     2041 agagaaggta cagtgctttc ttgcatttca gatgttgaag aaatgctaaa cgactgaata
     2101 cttgattggc tatgttgcga atctacatta ccagtagaag tattagtaat ggaagtagta
     2161 gaagtacttt tagttgaacc aacctcagtt gttgaaatgg ctgctgaaga ttctttaaca
     2221 gaattctttg ttttatatcc acaggcagaa aaaagtatta cagttcccaa caagcctgcg
     2281 taaatccctt tatgcatgat agctcctcct aaaatagatt atctttattc agtatatcac
     2341 ttaaacaaag aaaagcagat ggtttaaaat gaaaaaacct tcccaataaa gggaaggttc
     2401 atgaagataa taactttttt aataattata gctttctaat atctttcatc aacttttcta
     2461 gtaatcattg ggaaaaattt caagcagtag atcgcacaaa tacctacgac gatataaatg
     2521 atcttcgcaa aaatagttgt tgaaccacca gcaatcatag ctactaaatc aagttcgaac
     2581 aagcctacca acagccagtt caaccctccc acaatcaata aagttaatgc aatagcatct
     2641 aaagttttca ttagaattct cctttcttaa atgataaaac aattatatat caaaaagaga
     2701 aacattgctt atataacgct caggaatttt agcaatctat gcaaaaagat gaaaattttt
     2761 tttgctcttc cattagtaca cattttttag ctgaagctgt aaatttcatg tcttaatttt
     2821 ttttagtgag cttttatact ggatttgtaa gaaaaaagaa ggagaggatc acatatgtcg
     2881 gattggttgg atattgcagg aaaagtagta attgtaactg gaggttcctc aggaattgga
     2941 agaagtatcg tcgaaaattt gttgaagcag aatgcacaag tagcgaattt tgatgtaact
     3001 gaatgccgta tacagcatga aaacctacta tctttaaaag tcgatgtgtc ttctaaaacg
     3061 gacataaaag aagggatata taaagtaatg aaacatttta agacgatcga tggtctagtc
     3121 aataatgcag gaataaatat tccttctctt ctaatagata gaaatcatcc aaaaagcaag
     3181 tatgaattat cagaacaagt ttttgataag atgatcgctg tcaatcaaaa aagtgtatat
     3241 ttgatgagcc aagcagttgg aagaatcttg gtgcaaaagg gatcaggagt gatcgtgaat
     3301 ctttcttctg aatcaggatt ggaaggatca gaaggacaga gctgttatgc tgctacaaaa
     3361 gcagcgatga acagttttac tcgttcttgg gcaaaagagt taggaaagag gaatgttcgt
     3421 gtggtaggtg ttgcaccagg gatactggaa gaaaccggtt tgagaacaca agaatatgaa
     3481 gaagctcttt cttatacgag aggtataagc gtcgaacagc tgagaaacgg ttacagtcga
     3541 acaagtacga tccctctagg aaggagtggt aagctgcaag aagtggcgga tctagtctgc
     3601 tattatcttt cagaaagatc aagttatatc actggagtca cgactaatat ttcaggaggt
     3661 aaaacaagag gataaaggat aggggggacg cacgtgacgc tagtaaacag atggtaccaa
     3721 ttattgaaat tactagtcac tcacaagaaa gtttcaatcc aggaagtgca aaaagaaatc
     3781 aaaaccagta atcaaaccat aaaaaaagat attggggagt tgaatgaaga aattgccggt
     3841 attgcaaaaa tcatccaaaa aaacaaacat tttgaacttg aaatttatga ttttgatttt
     3901 tttgatgaga tcatgcaagg aaaattgaaa acagccagtg atttcaattc ggcatccaaa
     3961 agaatcgctt atttactaaa taggctgatc gaaacagagg atttcttacg tatggatgat
     4021 ttgtctgaag aagtcggtgt cagcagagga acagtggtca aggatctaaa gacattaaaa
     4081 gagatgatca atgattttga tgtaaagatt acaggcacac caaataaagg actaagaata
     4141 ataggagatg agctagaatt acgactactt ttattttatt ttgtttctcc ctacttttca
     4201 gagacgaaaa cagaacagtt tctagaagaa aaacaagcaa tcaaacattt tcagaagaag
     4261 acgaatgcta gtaaacaaga aatcaccctt ttaacgaaag ttatcggtat ttctttgaat
     4321 agaatctcta gtaactatct gttgaagaag cccatcaatc gttacagtgg gtgttttgaa
     4381 tatatccaag aattcaatga actgatcaat cagttggaag aaatctatga actaactttg
     4441 agccaatatg aaaaagattt tatcagtttt cctttgaaca tcatcaacac aagtataacg
     4501 atgataagga aagctaactc tgatttgcgc ttatatttcg accccatgat gatgcgtatt
     4561 cgacgattag atctcattga tctggatgag tccttcttat atcaagagat gaaacagcac
     4621 ctctctttat tgataaatcg ggtaattttt cgttatcgat tgactgattt attttatggt
     4681 gagatagaaa aaaagtatcc tttttcttac caaatcgcta gcgaatgcat acaagcatta
     4741 gaagctatct tgcaacataa gatctctaaa gtagagatca gctacctaac gttgtatttg
     4801 gagttagcat ttcgatatca aaaagagcat ttttcctcta aaaaagtagc cattgttcat
     4861 acgacaggaa aaggaacagc attaatgatc caaagacaaa tcaaaaggat attggggagg
     4921 gatatacaaa taacacatgt ttcagaagaa caatatgaaa aagtagatct aaacaaatat
     4981 ttcgctgttt ttacaacgat tccgttaaaa aatgtaaatg aagagacacc ggtgatccat
     5041 ttgactaatc tttttgatga ctcttggtta agggcacaat ggcaaaaagc aaaaaaaaag
     5101 cgattagccg ggtttcattc tactgttttt ttctatcaaa tatggaaaga aaaaaagacg
     5161 tatcaagaat atttaaacaa attagtcgct cctttgatca gaggctatta tgtggatgaa
     5221 gaatttgaaa aaaggatctt gaaaagagaa tatcagcaaa gcacatttgt taacaaagaa
     5281 tttgcttttc ctcacacaac caaccagcta tctcaacaca ttgttttatc agtagggcta
     5341 ataactgatc aaggtttagc agaaacagga ggaaatggag ttattttttt atttgccata
     5401 cctgaagaaa tgacggctga agttgagaat gaattatttg agctatacga tatcctattt
     5461 gaactcataa aaatgggaat agatgagttt attgaattag caccgacaca agaatcattt
     5521 ttaaaactaa tcaaaagtaa aggagagaga cttcatgaat gatatgtatc tattcatagt
     5581 tttcgcagct tcagcttatg tgctgcaagc agttatcagt tggaagcaac taaagcattt
     5641 caatcaaaca tatagcgaac tgcgaagaaa aggaaaagta gcgattggta aacgacctgg
     5701 gaaaatccgt tcaggcacga ttgttatgct agctatagac gatcatgatc aagttttaga
     5761 agtcagaaaa atgcaagggg ttacatcttt ggcaaaattt cattcattac ctgtctatgc
     5821 gggtgaagat atccattaca tcgatcgtta ccatccctca gttcgagcgg aagatcgttt
     5881 gacacaagca gctttagaaa atgccagaga agtatactta cgaatagagg caggaaatga
     5941 aaaaagtgaa caaccttcat tgccttttat tgctatgctc aataattttc ggatatggaa
     6001 aagtacgatc aaagaaaaaa ggagtgacaa acgatggata tcataacaaa atttgctgag
     6061 ggatttatga ggctgttcca aacaggtgca gaaacattca taagctggat gacaggaatc
     6121 gtaccagttg tcttgatgct gatggttgca atgaatacgc tgattgcttt tttaggtgaa
     6181 gaccgagtaa cgaaagtagc caaattatca gcgaaaaatc cgcttactcg ttaccttgtg
     6241 ttaccgttct tatctgcttt tatgttggga aatcctatgt catttacaat ggctcgtttc
     6301 cttccagaat actacaaacc aagctattat gctgctcaag ctcaattttg tcatacgagc
     6361 aatggtgtat tcccccatat taatcctgga gaattgtttg tatggatggg gattgctcaa
     6421 gggatcgaaa ccttagggct aaattcgatg gagctggcta tccgatactt gcttgtggga
     6481 ctgctgatga actttattgg tggttggata actgatttta ctactggatt tgtatgcaga
     6541 cagcaaggaa tcgttcttag caaaaaagta gagctttcag ttgattagag aaaggagagt
     6601 tttaaatgta tggtgatatt aagatcgtaa gaggaaacgg cgggtatgga ggtcctttga
     6661 ttattaggcc gacaaaagat aaacacaagt tcatctatgt cacaggtgga ggtgagaggc
     6721 caatgatcgt agataaaata gaagaactga caggtatgga gtctatcaat gggttcaaaa
     6781 catcaatacc tgatagtgaa atcgcgcttg caatcgttga ctgtggaggg actcttagat
     6841 gtggaattta tcctaaaaaa gggataccaa ccatcaatat cgttcccaca ggaaaaagtg
     6901 gcccattagc acaatatatc actgaagata tttatgtttc agcagtaggg ttagaacaaa
     6961 tacagccact aatggaaact gatgataaca aagaaacagt aaatactgca tctccagata
     7021 aaaagtacaa atatgatact tctaaaaaga taaccgagca aaaagcagag cagcaaaact
     7081 ggatagcaaa aattggaatg ggcgcgggaa aagtagttgc tacattcaat caatcagcaa
     7141 gagaagcagt agatacgatg atccggacag tgatcccttt tatggctttc gtttcccttt
     7201 tgattggtgt gatacaaggt tctgggatcg gtaatgtatt tgctaacatt atggcacctt
     7261 tagcaggaaa tgcagttgga ttaacaatca ttggctttat ttgttcactg ccatttttat
     7321 ctcctttgct tggtccaggg gcagttatta gccaagtgat tggaacattg atcggtgtag
     7381 agattgggaa aggaaatatt cctccccaat tagcattacc ggcactattt gctatcaata
     7441 ctcaaaatgc atgtgatttt attccagtcg gtctaggttt ggaagaagca aaagctgaga
     7501 cagtagaagt aggtgtacca tcagttcttt attcaagatt tcttaacggc atcccacgag
     7561 ttatcattgc ttgggtagca agttttggat tatatagctg
//
LOCUS       DACEEZ010000109         7408 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20313_2017
            SAMN12639966-rid9879223.denovo.109, whole genome shotgun sequence.
ACCESSION   DACEEZ010000109 DACEEZ010000000
VERSION     DACEEZ010000109.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639966
            Sequence Read Archive: SRR10023158
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7408)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7408)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741683.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741683.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:44:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,016
            CDSs (total)                      :: 2,962
            Genes (coding)                    :: 2,889
            CDSs (with protein)               :: 2,889
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 36 of 73
            Pseudo Genes (incomplete)         :: 29 of 73
            Pseudo Genes (internal stop)      :: 18 of 73
            Pseudo Genes (multiple problems)  :: 8 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7408
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639966-rid9879223.denovo.109"
                     /isolate="E20313_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(13..348)
                     /locus_tag="IWO89_002556"
     CDS             complement(13..348)
                     /locus_tag="IWO89_002556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010756787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HAQ0457260.1"
                     /translation="MKMNTNDIATVYVAFTAAEGGKRRPVYILKNGLDTISFLSITSK
                     YHTKSDKIKLKYVEIRDWKQAGLKKESWIDVGTLNELPKNNKKVKWKKIGELSPSDLQ
                     RLEERMEEL"
     gene            complement(332..604)
                     /locus_tag="IWO89_002557"
     CDS             complement(332..604)
                     /locus_tag="IWO89_002557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelB/DinJ family
                     antitoxin"
                     /protein_id="HAQ0457261.1"
                     /translation="MEKMAKLQISMDEKLKNEAESIIDELGMSPKTAVTVFYKQIVEQ
                     GKIPFSIELSEKSKRIHNIQRLAEKLPVQKLDTDEKIEEWFNEDEY"
     gene            complement(1055..1858)
                     /locus_tag="IWO89_002558"
     CDS             complement(1055..1858)
                     /locus_tag="IWO89_002558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HAQ0457262.1"
                     /translation="MRFLHLSDIHFRENYQTNLFTAELFPTIKNPQLALEKLLQELSL
                     TVYDFILLTGDLVHEGGISDYQVLRKMLDQYFPTTPYYFCRGNHDRRSVFFEGMSVES
                     NETGEYIHCTNQNGLRIISLDSAQDTQHEGKISIGQMIQLQEWLGTPAEKGTILLVHH
                     PLSWEDPSIVTEVPEGFAELIAESDILGIFVGHIHQGSSAEFAGKKQFMAESLSFGVD
                     EYPDYSLFTNRTGFTSGSLIDGKIFTYQHYLTPHQIEIGRMKKPITGNH"
     gene            complement(1836..2960)
                     /locus_tag="IWO89_002559"
     CDS             complement(1836..2960)
                     /locus_tag="IWO89_002559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ0457263.1"
                     /translation="MGETLELRNIGKKYGEHTILKEIDLAVAAGERIVLLGPSGSGKS
                     TLLRMIAGLEKITSGELFLGTQLANKLPCGEREIAMVFQNYALYPHMTVKENIIFALR
                     ANKIKTADIQHRLEEALKMLGLTPYQDRLPKDLSGGQRQRVALARAVVKRSNYFLLDE
                     PLSNLDVRLRLDARKELVKIHQRYKQTFVYVTHDQIEAMTLADRIVLLNEGRIQMIDT
                     PKNVYTKPKNTFTANFIGSPGMNIMPVTYDQGTLKVASQFLTLNPAWQKQLLQVSGSL
                     YLGIRPEHVTFSKETSDLHGQVKYRELLGQSYAYTVKVDGIEMIVLNEKPTWQIGDAV
                     NLRIEPERMHFFDYQTQENLGYPDYFIEERGNSHALFASL"
     gene            complement(2976..3791)
                     /locus_tag="IWO89_002560"
     CDS             complement(2976..3791)
                     /locus_tag="IWO89_002560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006739361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HAQ0457264.1"
                     /translation="MKIKRWLPHLLFMFIIIIIIYPLLFIMANSFKPLKDAYQTITSL
                     IPQPFTLENFCYLFENLPIIKITRNTFFIATIITVVKLGVAFFAAYSFTYCRIKGKKI
                     WYFIIIGTMFIPFTVAMIPNYLLIAKLNLIDTTWGVIIPQFADAMGIFLLTQTMREIP
                     VSLLDVAALDNIKQTTVMSKIVFPLTRHAITSTGIWFFITSWNEYVWPVLILRTVDNY
                     TLPLAMQTYISSEGGTNFTVAMAISLITMFVPLVLYAIFQKYIIGTFVSAGIK"
     gene            complement(3781..4662)
                     /locus_tag="IWO89_002561"
     CDS             complement(3781..4662)
                     /locus_tag="IWO89_002561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006739050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAQ0457265.1"
                     /translation="MSEMKRYVGYLYLAPAIILLGVFVFYPLIYTIYLSFFDWNMVAP
                     TKEFVGFANYVKVLTDPVFHKVLKNTLSYTVLFVLLSCVFPYITSFLLGIVIEKGQNL
                     YKPLFFIPAVISLVVAGMVFTWILNPVSGPIAVLLGNFGLRLPMWMNLQGWVIAVIAG
                     ITAWKVFGYNFIVIFAAIVGIDQEMIEAAKLDRVPIWRIFFTIVLPMSSSTGLYLLVM
                     TIVQGLQYVFTPIKIITRGGPNNGSANVIYYAYQKAFEMYKVGESSAISVLTLLIFAV
                     FLGVIFKFVEKGVYYEN"
     gene            complement(4738..6027)
                     /locus_tag="IWO89_002562"
     CDS             complement(4738..6027)
                     /locus_tag="IWO89_002562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002581297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HAQ0457266.1"
                     /translation="MLKKNLVIGVVSVLSVISLAACGSGNTKTTKKSEGRVTIEYWHV
                     NADTLGGKTVNELVDKYNQSQDKVKVVAKYNPDMYKGLMQNLQASVASKEIPDVVQVG
                     WAFKDYFAENFKYTNPQSLVEEVDPDNADYFKDHFLDNIMALSEKDGKQVGIPYSISN
                     PVLYLNQDILKAAGLDENGPKTWEEVQTFSKIIKEKTGKYGVYIQEPADNWAQQAILE
                     SNGASVIKDGKATFASKDGIAAYELYQRMVKDQTALHTTWEQGVQSFIDGNVAMVYTT
                     IAQRDNIQKNSTFTAKAVESPSWEGKKVKLPAGGAMLAVTSQTDKKKEATWDFLKYLY
                     SVESLAKWTEGTGYLPPRKDVADAQNGLKAFLAENQMMIPAIDQMDKMVSWSSFPGDA
                     GLEAEQKLLDMRDKILSGNSVNETMKATQKEINELLK"
     gene            complement(6255..6899)
                     /locus_tag="IWO89_002563"
                     /pseudo
     CDS             complement(6255..6899)
                     /locus_tag="IWO89_002563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303552.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
BASE COUNT         2278 a         1589 c         1136 g         2405 t
ORIGIN      
        1 tttttagtat tattataatt cttccatgcg ttcttccagt cgctgtaaat cgcttggact
       61 taattcccca atttttttcc atttgacctt tttgttgttt ttgggtaatt cgtttagagt
      121 tcctacgtca atccaagatt ctttttttag tcctgcctgt ttccaatccc ttatttcaac
      181 gtatttcagc tttattttat cggatttagt atgatatttt gaagtaattg acagaaacga
      241 aattgtgtcc agtccatttt ttagaatata gacaggccgg cgtttaccgc cctctgcagc
      301 tgtgaaagca acgtagacgg ttgctatatc attagtattc atcttcattg aaccattctt
      361 caattttttc gtcagtgtct agtttttgga caggcaattt ttccgctaat ctttgaatat
      421 tatggattcg tttacttttt tccgacaatt caatagaaaa tgggattttt ccttgctcaa
      481 caatttgctt ataaaaaacg gtgacggctg ttttggggct catgcctagt tcatcaataa
      541 tgctttctgc ttcatttttc aatttttcgt ccatagaaat ttgtaactta gccattttct
      601 ccacctcttt ctgacaaaca cattatatca cggaaagagt tcaaatggaa ttaatacaac
      661 tacttttgaa ctccttgaaa tgcagtgtat ttatctaaat ataaggaaag gatatagtgc
      721 taaatggcct tgatagatag aaaagtttca aaatccgcca cagcgataat tgaagcgaac
      781 actcaacgac acacagaaca ggaaaagact cctaaaagaa ccaaattaac taggacgtgt
      841 ctgaaaactc cacaaagatg tggtatgctc tggtaaaaaa cgaatggcac aaccacgaag
      901 accgatatgg ttcttggaaa actttctcca ccctttttgt aattggaggg atagtggttt
      961 attgatagcg attttccaag ttcttcaagt ggaaccagac attgagcacc acggctaaaa
     1021 agtgaatgaa gtctttattt ttaatcaata atttttaatg attaccagta attggttttt
     1081 tcatgcgccc tatttctatt tgatgtggag ttaaataatg ctgataagta aaaatttttc
     1141 cgtcaattaa actgccacta gtgaatcctg ttctattggt aaaaagagaa tagtctgggt
     1201 attcatctac accaaatgat aaagattcag ccataaactg ttttttacca gcaaattcag
     1261 cactgctacc ttgatggata tgtcctacaa aaatacctaa aatatcactt tcggcaatca
     1321 attcagcaaa accttccgga acttcagtta caatagatgg atcttcccac gacaatggat
     1381 gatgaaccaa taaaatcgtt cccttttcag caggtgtacc caaccactct tgtaattgaa
     1441 tcatctgtcc tatcgatatt tttccttcgt gctgggtatc ttgagcagaa tccaaggaaa
     1501 taattcgtaa cccattttga ttcgtacaat ggatatactc tcccgtttca ttgctttcaa
     1561 cactcattcc ttcaaagaaa acactgcgac gatcatgatt cccacgacaa aaatagtaag
     1621 gtgtggttgg gaaatattga tccaacattt tccgtaagac ttgataatca gaaatccccc
     1681 cttcatgtac taaatcacct gttaatagaa taaaatcgta cactgttaac gagagttctt
     1741 gtaacaattt ttccaatgcc aactgtggat ttttaattgt tggaaatagt tcagcagtaa
     1801 acaagttagt ttgatagttt tctctaaaat ggatatcaga gagatgcaaa aagcgcatgg
     1861 ctattccctc tttcttcaat aaaataatca ggatatccta aattttcttg cgtctgataa
     1921 tcaaaaaaat gcattctttc aggttctatg cggagattga ctgcatcacc aatctgccaa
     1981 gtaggttttt cattaaggac aatcatttct ataccatcga ctttaacggt gtacgcataa
     2041 ctttgaccta atagctcacg atacttcact tgaccgtgta aatcgcttgt ttcttttgaa
     2101 aaggttacgt gttctggtct tatgcctaaa tacaatgagc cactaacctg aagaagttgt
     2161 ttttgccaag ctggattcaa tgttaaaaat tgactggcta cctttaatgt tccttgatca
     2221 taagtaactg gcataatatt catgcctggc gaaccaataa aatttgcagt aaaagtattt
     2281 ttaggtttcg tataaacatt tttcggcgta tcaatcatct gaattctacc ttcattcaat
     2341 aaaacaatcc ggtcagctaa cgtcatcgct tcaatttgat catgagtgac gtagacaaaa
     2401 gtttgtttat aacgttgatg aatttttacc aattcttttc gtgcatccaa tcgtaaacgc
     2461 acatccaaat ttgataacgg ttcatctaat aaaaaataat tactccgttt aaccaccgca
     2521 cgagccaatg ctacacgctg ccgttgccct cctgacaaat ctttaggaag tcgatcttgg
     2581 tatggcgtta aacctaacat cttcaaagct tcttccaaac ggtgctgaat gtcagctgtc
     2641 ttaattttat tcgcccttaa tgcaaaaata atattctcct ttacagtcat gtgaggatat
     2701 aaagcataat tttgaaaaac cattgcgatc tctcgttctc cacaaggtag cttatttgct
     2761 agttgtgtac ctaaaaaaag ctctccactc gtaatttttt ccaaaccagc aatcattctc
     2821 agcaaagtag attttcctga accagatggt cccagtaaca caattcgttc tccagcggcc
     2881 acagctaaat caatttcttt taaaatggta tgttctccat atttttttcc gatatttctt
     2941 aattcaagcg tttctcccat gatttgtcct ccaatttatt taatacctgc agaaacaaaa
     3001 gtcccgataa tatatttttg aaaaattgca tacaagacta acggtacaaa cattgtaatt
     3061 aaactaatcg ccattgctac ggtaaaattt gtcccacctt cagaactaat ataggtctgc
     3121 atcgccaacg gcaaagtata attatctact gtccgtaaaa ttaataccgg ccaaacatac
     3181 tcattccaag aggtaataaa aaaccaaata ccggtagaag taatcgcatg tctagtaaga
     3241 ggaaaaacaa ttttactcat gacggttgtt tgtttgatgt tatccaatgc cgccacatct
     3301 agaaggctca ctggaatttc ccgcattgtt tgcgtcaata aaaaaatgcc catcgcatca
     3361 gcaaattgtg gtattatcac accccaagta gtatcaatta aattaagttt tgcaatcaac
     3421 aaataattag gaatcatcgc aacagtaaaa ggaatgaaca tcgtaccaat gatgataaag
     3481 taccagattt tcttcccttt gattcggcaa taagtaaaac tatatgccgc aaaaaaagct
     3541 acgcctaact taacaaccgt tataattgta gcaataaaaa aagtatttct cgtaattttg
     3601 ataattggaa gattttcgaa caaatagcaa aaattttcca aggtaaacgg ctgtggtatc
     3661 aaactagtaa tcgtctgata ggcatctttc aatggcttaa aagaattggc cataataaac
     3721 aataatggat agataataat gattatgatg aacataaata agagatgtgg caaccagcgt
     3781 ttaattttca tagtaaaccc ccttttccac aaatttaaat attaccccta agaaaacagc
     3841 aaatattaat aaagttaaaa ctgaaattgc tgaagattct ccgactttat acatttcaaa
     3901 agccttttga taagcataat agatcacgtt tgcactacca ttattcggtc caccacgagt
     3961 gatgatctta atcggagtaa acacatactg cagaccttga acaatggtca tcaccaataa
     4021 atatagtcct gtcgaactac tcataggcaa cacaattgta aaaaaaatgc gccagatggg
     4081 tacacgatcc aacttagctg cttcgatcat ctcttgatca attcccacaa ttgctgcaaa
     4141 aatgacaatg aaattataac caaaaacttt ccaagcagta atacctgcaa tcacagcaat
     4201 tacccaacct tgtaaattca tccacatagg cagtcttaaa ccaaaatttc ccaacaagac
     4261 agcaattggt cccgataccg gatttaatat ccatgtaaaa accatccccg ccactaccaa
     4321 agaaatcaca gctggaataa aaaataatgg tttatacaaa ttctgccctt tttctataac
     4381 tattcctaat aaaaaggatg taatatatgg gaacacacag ctcagcaaga caaatagaac
     4441 ggtataggac aaagtattct tcaacacttt atgaaagact ggatcagtca gcacctttac
     4501 atagttggca aagccaacaa attctttagt gggggccacc atattccagt caaaaaaact
     4561 caaatagatc gtataaatca atggataaaa aacaaacacg cccaacaaaa taattgctgg
     4621 cgccagatac aaataaccta catagcgctt catttccgac agcctcctca tccttatgtc
     4681 aaaagcagac gaccttgttt ttcctttctt acaggcgctg tcgtctgctt gctttgctta
     4741 tttgagtaac tcattaattt ccttttgagt agctttcatc gtttcattga cggaattacc
     4801 tgataaaatt ttatcccgca tatccaacaa tttttgttca gcttctaatc cagcatctcc
     4861 aggaaatgaa ctccaactga ccatcttatc catctgatca attgctggta tcatcatctg
     4921 attttcagct aagaaagctt ttaatccatt ttgggcgtct gcaacatctt tacgtggtgg
     4981 taaataacca gtaccttctg tccatttagc taaagattca acactataaa gatattttaa
     5041 gaaatcccaa gttgcttcct ttttcttatc cgtttgtgat gtaacagcta acattgcgcc
     5101 accagcaggt agtttaactt ttttcccttc ccaacttggt gactcaactg ctttagcagt
     5161 aaaagtacta tttttttgaa tgttgtccct ttgagcaata gtggtataga ccatcgcgac
     5221 gttaccatca ataaagcttt gcacaccttg ttcccacgta gtgtgtaatg ccgtctggtc
     5281 ttttaccatc ctttgatata attcataagc agcgatacca tctttagaag caaaggtagc
     5341 tttgccatct tttattacac ttgccccatt actttctaaa attgcttgtt gtgcccaatt
     5401 atcagcaggt tcttgaatat acacgccata ctttccagtt ttttctttta ttatcttaga
     5461 aaaagtttga acttcttccc aagtttttgg accattttca tccaagcccg ctgcttttaa
     5521 tatatcttga tttaaatata aaaccggatt actaatggag taaggaatac ccacttgttt
     5581 accatctttt tcagacagtg ccatgatatt gtctaaaaaa tgatctttaa aataatcagc
     5641 attatccgga tccacttctt ctactaaaga ttgaggatta gtatatttga aattttctgc
     5701 aaaataatct ttaaaagccc atcccacttg tactacgtcc ggtatttctt tggaggcaac
     5761 agaagcttgt aaattttgca tcaagccttt atacatatca ggattatatt ttgctacaac
     5821 cttcactttg tcttgagatt gattgtattt atctactaat tcattcactg tttttccacc
     5881 tagggtatcc gcattaacat gccaatactc tatcgttaca cgaccctccg atttttttgt
     5941 tgtcttggta ttcccactac cacaagctgc caaagagatg acacttaaaa cagacacaac
     6001 ccctataaca agatttttct ttaacataac attaacttcc tcctaatttc tttatggcca
     6061 tttttctaat caagaaatat tatgaactac caatgtaaat ttctagttaa ttttctgtaa
     6121 ataattcgaa atttgtgcat ttcggggtac agggtcactg gacatatcca tcaagctaag
     6181 aataaagccc ccatatggtc atgtatccca tctccaaatc tcttaactcc tctgttttga
     6241 ttataaaata gtttattctg tgctcctttc ttccataaat aggcccatgc acaacgtgag
     6301 gttatattat aaacaccaca gcgcgcaagg tactcaatcg tttctagttc attgactgtc
     6361 catacagaaa atgtcaatcc tagctgctgc cccacttcac gggccaactt agttaaccat
     6421 ccttcatgaa tattgacgat ttgaacgctt tgataggcta atttctgtaa ggtatcgatc
     6481 aaatctgctt cggtaatatc cccaagtttt ttcccttcga actgattttc aatattataa
     6541 aatatatgct ttccccaaat cgcagcccaa gataattgta catagccact aagaacaatt
     6601 tgtggccaga tttgataggt ttgtgccagc gaaaaaacgg ccatttccaa tccctcttct
     6661 tttaaatcac agttaatggt catggttgcc ttctcattgg ccactgcttg aaacacctgc
     6721 tcaagggtta ccgaatctgg gacaacttct tacctgtcat gggctagtat tagcgtacca
     6781 tctgtttgct gacgcacatc tacttcaaaa caaagctggc gatccaaaca aaagcgaaga
     6841 aatgcccagc tattctcctc cgtattgtca ctaccagagt gtgcagtgat catggtcatt
     6901 ttgacccctc ctttaattcc gtgattttct cccacacgtc cactataccg atgaagcgtt
     6961 aatttcttgt taatttcttg taaattccct gcgttttttc agtaaactag ttgtaaatct
     7021 tgcatgctcc ttcctacgaa ctcatgaatc gtcgattttg cccttctagc tagtttcacc
     7081 aattaagtag ttctacattc cccgctttta acctactaca cctttttttg ttttcagcta
     7141 ttccttcttc ttgctgaaag tccttgaggt tctttcttac aggataccat caaggtgttc
     7201 aatggcttat tcccattgaa tgaccgagac ttactatcag atttctgctc gttgaaccaa
     7261 gtctttgata caggatttct tccgtcatct tctcacaaat accaccttgc gtttctctag
     7321 cgattggtcg atgtgcaccc tcccagtgga ctgtcactac ccctttttgc tatgcctagc
     7381 ccacaaaaaa agctgtcctc acagacag
//
LOCUS       DACEEZ010000110         7369 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20313_2017
            SAMN12639966-rid9879223.denovo.110, whole genome shotgun sequence.
ACCESSION   DACEEZ010000110 DACEEZ010000000
VERSION     DACEEZ010000110.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639966
            Sequence Read Archive: SRR10023158
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7369)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7369)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741683.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741683.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:44:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,016
            CDSs (total)                      :: 2,962
            Genes (coding)                    :: 2,889
            CDSs (with protein)               :: 2,889
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 36 of 73
            Pseudo Genes (incomplete)         :: 29 of 73
            Pseudo Genes (internal stop)      :: 18 of 73
            Pseudo Genes (multiple problems)  :: 8 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7369
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639966-rid9879223.denovo.110"
                     /isolate="E20313_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(<1..2292)
                     /gene="esp"
                     /locus_tag="IWO89_002564"
     CDS             complement(<1..2292)
                     /gene="esp"
                     /locus_tag="IWO89_002564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="repetitive surface protein Esp"
                     /protein_id="HAQ0457267.1"
                     /translation="MVSKNNKRVFLEKTKKRVLKYSIKKLSVGVASVLIGVGLVLGTT
                     ELVKAQDEISPSSPLETAISSVQVGDKVASGNTFQENPGYTKNYNFSDLQFSPQELTG
                     DTLKGNTIGFEVYGKHNIAASTKNWEIRLQLDERLAKYVEKIQVDPKKGIGSSRRTFV
                     RINDSLGRPTNIWKVNYIRASDGLFAGAETTDTQTAPNGVITFEKSLDEIFKEIGIDN
                     LKTDRLMYRIYLVSHQDDDKIVPGIDSTGYFLTDSDDFYNSLDVSENNPDQFKHGSVS
                     AKYEKPNTQTKDGSGSTGANGAIILDHKLTKNYNFSYSSSAKGTPWYANYKIDERLVP
                     YVAGIQMHMVQADKVTYDVSFESGTKVADLAIERREGHENYGIGSITNNDLTKLIDFA
                     NASPRPVVIRYVLQLTKPLDEILEDMKATAQVEENKPFGEDFIFDSWLSDTNKKLIQN
                     TYGTGYYYLQDIDGDGNPDDKEESGDTNPYIGKPELEEVYDVDTTVKGKVFIHELAGT
                     GHKAQLVDKEGTVLAEKTIAPNEKDGAPISDTVEFEFTGVDSSKLIAKDELKIQIVSP
                     GFDKPEEGSTVIKESPKAVDKQTVVVGFKPDAKESIRNNKNLPEDAEYSWKTEPDTSN
                     VTDSTKGIVTVKIGNRTFDVDVEFAVKASQAMENDATYVPITTTPETTVQSGKPTFDK
                     PDVPLAKDAFSISDVYNKYFGNASVDANTGIVTFTPAKGVGESEPITGTIPIKIVYQD
                     GSVGTTDLAVTVSKDIYENPGENI"
     gene            complement(2909..3343)
                     /locus_tag="IWO89_002565"
     CDS             complement(2909..3343)
                     /locus_tag="IWO89_002565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0457268.1"
                     /translation="MRLYHLRSLEELRKLDFNKKFFWEISDLSCFVESERLSNGNNSV
                     LNEYDKVVINKVKSLCEEARESISHINGSNFKQTLFNLIKLDAKISSYLFFLIHDEFM
                     DYQKLDQLIERESWEDYYVGLTFSEKLNNYKLIDYKYLSESN"
     gene            complement(3360..4565)
                     /locus_tag="IWO89_002566"
     CDS             complement(3360..4565)
                     /locus_tag="IWO89_002566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YSIRK-targeted surface antigen transcriptional
                     regulator"
                     /protein_id="HAQ0457269.1"
                     /translation="MFSDKEVLLKSLHVCLKLPIVVFNSEFSIIKEYRSDRTTYFFYD
                     YKKLLTDNIKKTKDFHFFTGDFNEMFLLYMFKTRYYLFGPFRANNIDKVFFKLKMNNL
                     NVAMADRERLYNSLQNLTLYSLGDIRDILILVHYFFTGKIEDLFHEPLIEYTENLSKT
                     IEQIKIDNLLSQNYDPEIYLFLYENKILEYVKNGDIRNLENMVFNLSNGIIPSVSGDT
                     IRSEKNYSIIVFEKLAQTSITLGMDIIEAYQSRDALIQENELAVSLPEVLKVRDSGIV
                     YYTKEIGKTKIEHLSPLISSVVQFIGLNIYKRITVKEIANYFSVSETKLRESFKNEMH
                     ITIYNYISKRKISTAKIMLKSNHTISEVSLGLGFSDSSHFSRVFKKYAGVSPKQYQLG
                     LVDNFNNIS"
     gene            5259..5645
                     /locus_tag="IWO89_002567"
     CDS             5259..5645
                     /locus_tag="IWO89_002567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HAQ0457270.1"
                     /translation="MSDNDERVNAQLIRFFEKVILNTANTYYKKQEEIQKHEQLDQLP
                     PYLFTKDTIKFKQPINVLNVTLLVEDIKLAKILPLLKEPEQIFLIEKFIFDKTDKEIG
                     KVLGITRQGATNLKHRLYKKIQKMLV"
     gene            5739..5930
                     /locus_tag="IWO89_002568"
     CDS             5739..5930
                     /locus_tag="IWO89_002568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAQ0457271.1"
                     /translation="MNSLKEDFILAKAGNEEAVEAILKRFSSLIHKQSWRTGKYDQDC
                     YQECMLAIYLAISKFEIKE"
     gene            6444..6776
                     /locus_tag="IWO89_002569"
     CDS             6444..6776
                     /locus_tag="IWO89_002569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HAQ0457272.1"
                     /translation="MRKTYDRKFKLKISQDILEKKVTTKKIAEEYNISRPTISRWVSE
                     YRRYGKNAFAGQGKRLPDKADFYILEQENKRLKEEDECSTNTKIDTDLQGSLKNFTMK
                     ESKSINVL"
     gene            7072..7227
                     /locus_tag="IWO89_002570"
     CDS             7072..7227
                     /locus_tag="IWO89_002570"
                     /inference="COORDINATES: protein motif:HMM:NF012870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase family protein"
                     /protein_id="HAQ0457273.1"
                     /translation="MPTVIHSDRGSQHRSFIYQELLNDHHITHSVSQTGTPVDNAVIE
                     AFHRSIK"
BASE COUNT         2455 a         1296 c         1056 g         2562 t
ORIGIN      
        1 aatgttttct cctggatttt cataaatatc tttacttaca gttactgcta aatcggtcgt
       61 gcctacagaa ccatcttggt aaacaatttt aataggaatt gttccagtaa tcggctccga
      121 ttctcctaca cctttagctg gagtgaatgt aacaatacca gtgtttgcat caacacttgc
      181 attaccgaag tacttattat agacatctga aattgaaaag gcatctttag ctaaaggaac
      241 atctggttta tcaaatgtag gttttccact ttgaactgtc gtttctggag ttgttgttat
      301 aggtacgtat gttgcatcat tttccatagc ttgagaagct tttacagcaa actctacatc
      361 cacgtcgaaa gttcgatttc cgatcttaac agttacaata cctttcgtac tatcagtaac
      421 gttagaagta tcaggctctg ttttccatga atactctgca tcttcaggta agtttttatt
      481 attccgaatt gattctttag catctggttt aaatccaact accacggttt gtttatcaac
      541 cgcttttggt gattccttaa taacggttga accttcttct ggtttatcaa aacctggaga
      601 aacgatttgg atttttaatt catctttcgc gattaattta cttgaatcta cacccgtaaa
      661 ttcaaattct acagtatctg aaattggagc cccatctttt tcatttggag cgatagtttt
      721 ttctgctaat acagtacctt ctttatcaac aagttgggct ttgtgacctg ttcccgctaa
      781 ctcgtggatg aatactttcc ccttaactgt tgtgtcaaca tcatatactt cttctaattc
      841 aggtttcccg atatatggat tcgtgtctcc gctctcttct ttatcgtcag ggtttccatc
      901 accatcaata tcttgcaaat aataataacc tgttccataa gtgttctgga ttaatttttt
      961 attcgtatcc gacaaccaag aatcaaagat gaaatcttca ccaaatggtt tattttcttc
     1021 aacttgtgct gttgctttca tatcttctaa gatttcatct aatggcttag tcaattgtaa
     1081 aacatatcga ataacgactg gacgtggact tgcattagca aaatcgataa gtttagttaa
     1141 atcattatta gttattgaac ctatgccata attttcatga ccttcccgtc gctcgattgc
     1201 taaatcagct acttttgttc cggattcaaa cgaaacatca tatgttactt tgtcagcctg
     1261 aaccatgtgc atctgtatac ccgctacata tggaactaat cgttcatcaa ttttgtaatt
     1321 agcataccaa ggcgtgccct ttgcggaaga tgaatatgag aaattataat ttttcgttaa
     1381 cttatgatct aagattatag ctccattagc acctgtggat cctgaaccat cttttgtttg
     1441 agtattaggt ttctcatatt ttgcacttac tgatccatgt ttaaactggt caggattatt
     1501 ttcagataca tctaagctat tatagaaatc atctgaatct gttaaaaagt agcccgtact
     1561 atcaataccc ggtacaattt tatcatcgtc ttgatgactt accaagtaga ttcgatacat
     1621 taaacgatcg gtcttgagat tgtctatgcc tatctcttta aaaatttcat ctaggctctt
     1681 ttcaaacgta attaccccat taggagcggt ttgcgtatcg gttgtttctg ccccagcaaa
     1741 taatccatca cttgcacgaa tataattgac cttccaaata tttgtaggtc tacccagcga
     1801 atcattaatt cttacaaagg tccgtctaga agatccaatt cctttctttg gatcgacctg
     1861 aattttttct acatatttag ctaatcgttc atctaattga agacggattt cccagttttt
     1921 agttgatgct gcaatattat gttttccgta aacttcaaat ccaattgtat tgccttttag
     1981 tgtgtctcca gttagttctt gaggactaaa ttgtaaatcc gaaaagttat aatttttcgt
     2041 ataaccagga ttctcctgaa aagtattccc cgaagctact ttgtcaccta cttgtacgga
     2101 gcttatcgct gtttcaagtg ggctgcttgg agaaatttca tcttgtgctt taactagttc
     2161 agttgttccc aagacaagac caacaccaat caaaacagat gcaaccccaa cacttaattt
     2221 tttgatactg tacttcaata ctcgtttctt tgttttttct agaaatactc tcttattatt
     2281 cttgctaacc attattcaaa tcaactccct taataattac tttcctagta agaataaaag
     2341 ataaatctga tacgtattat tcacagaata ggaatcttac taaccttatg ttaactaatt
     2401 tacttagaga atttcatgaa cattacaaca aaaaaagtac atttcttcat gtttttttcg
     2461 gatatttgtg ctttttaacc attcagcagc tgaaaacata taaaaagata tgtatgtatt
     2521 ctttctgaaa ttaagttcat ttctatgcga tgaagcatgt tttcaaaata ttaccctttc
     2581 ttagattaaa cttcagagta ttcaaataac ggaataagga gccttatccc gcattactga
     2641 atgagactgt gacataagta aaaaggtgac ttcaaattcc ttcttcggaa tttgaaatca
     2701 ccttctttat aattataaag tatcggtttc acagcatttc tgaatgattc ctagaatctt
     2761 aaacgaatga ttggagttat caacattttt tcactcgttt ttagcgaaac aaaaaatgac
     2821 atttgtcaca gcctcattaa attattgatt ctttttcttt ataattgaat tgtccatatt
     2881 taaagtgtct agtactctac atgaataatt aattgctttc actcaaatac ttataatcta
     2941 ttaacttgta gttgtttaac ttttctgaaa aagttagacc cacgtaataa tcttcccagc
     3001 tttctctttc aattaactga tccaattttt gataatccat aaattcatca tgtatcaaaa
     3061 agaataaata acttgatatt ttagcatcta gcttaatcag attaaataat gtttgtttaa
     3121 aattgctacc attaatatga ctgatagatt ctctagcttc ttcacataaa ctttttactt
     3181 tattaattac tactttgtca tattcattta acacactatt attaccatta cttagacgct
     3241 cagattctac gaaacaactc aaatcagaaa tttcccaaaa aaattttttg ttaaaatcca
     3301 atttcctcaa ctcctccaaa ctacgtaaat gatatagtct cataataaaa taaaacctct
     3361 cagctaatgt tgttgaaatt gtccactaaa cctaattgat attgcttagg agatacacct
     3421 gcgtactttt tgaatactct cgaaaagtgt gaagaatctg aaaatcctaa acctaaagaa
     3481 acttcactga tagtatggtt tgatttcaac attattttag ccgtagaaat ttttcgctta
     3541 gagatataat tataaatcgt aatatgcatt tcattcttaa agctttctct taatttagtt
     3601 tcacttacag aaaaataatt agctatttcc tttacagtaa ttcttttata gatatttaag
     3661 ccgatgaatt gaacgacaga agaaattaag ggagaaagat gctctatctt agtttttccg
     3721 atttccttag tataataaac aatcccagaa tccctcactt ttaagacctc aggtaacgat
     3781 acagctaatt cgttttcttg tatcaaagca tctctacttt gatacgcttc tattatatcc
     3841 attcctaatg ttatagacgt ttgtgctaac ttttcaaaaa caatgataga ataatttttt
     3901 tcagaccgaa tcgtatctcc actaacactt ggaattattc cattactcag attaaaaacc
     3961 atgttttcta aatttcgaat atctccattt ttaacatatt ccagtatttt attttcataa
     4021 agaaacagat atatttctgg atcgtaattt tgtgataaca gattatctat tttgatttgc
     4081 tcgatagttt tacttagatt ttctgtatat tcaattaacg gttcatggaa tagatcttct
     4141 atttttccag taaaaaaata atgaactaaa atcagaatat ctcgaatgtc tcctaaagaa
     4201 tacaaagtca aattttgcaa tgaattatag agtcgttctc tatcagccat tgcaacattc
     4261 aaattgttca tttttagttt aaaaaaaact ttatcaatat tatttgctct aaatggacca
     4321 aataagtaat atctggtttt aaacatgtat agaagaaaca tctcattaaa atcaccagta
     4381 aagaaatgga aatctttagt ttttttaata ttatcagtta gtaacttttt ataatcataa
     4441 aaaaaataag tagttctatc tgagcgatac tcttttataa tactgaattc ggaattaaaa
     4501 accacgatag gtaactttaa acaaacatga agagacttta ataagacttc tttatctgaa
     4561 aacacttttg tttctctctt tcctttctac atcattaaat agtattttat acgttatcta
     4621 aaatactatt cctctgtcaa aataagctaa cttacttcat tataaaatat ccacttatcg
     4681 ctttcatgta tattccaccg taaaaagtac attttatcac cgagaactca tcatttttca
     4741 tcatgatgaa tactatttac atatatctag ttcaaagaca ataattatta tgatccgtta
     4801 ttatctaagc actatacaga taataggaat ttttcgtttc taaatattcc aatttgggaa
     4861 tccaataaaa attttcgttc aaatacgtgt ctattcgaac tcttttctag ttgttaatta
     4921 tgaaggggga agaaataaaa aatacaatct atacacgaat aagaatatta tccaaaagga
     4981 ggttttagaa aagatttatt atacgtaaaa agctcaagac attttcttag ccataaggac
     5041 atgtttgata tttaagaaga tatatgaatt ttcttttcta agtataaaga taactatcca
     5101 ttatcaacta aaaaaacatt tttgaaaata aacatcagca gtcctatcaa tgcatgtgag
     5161 cttatagaag ttattcgcag cgcagcataa ttgcattagc ttcttttcgt gttttttgcc
     5221 tcttttccct ttaaatctta atggaggagg tgtgcaatat gtcagataac gacgaacgcg
     5281 taaatgccca gttgattcga ttctttgaaa aggtcatttt gaacaccgct aatacttatt
     5341 ataaaaaaca agaagaaatt caaaaacatg aacaacttga tcaactccca ccatatttgt
     5401 ttactaaaga tactataaaa tttaaacaac cgattaatgt tttaaatgtg actctccttg
     5461 ttgaagatat aaaattagca aaaatccttc ctttgttgaa agaacctgaa caaatttttc
     5521 ttatcgaaaa atttatcttt gataaaactg ataaagaaat tggaaaggtt ctggggatta
     5581 caagacaggg tgcgacaaat ttaaaacatc gactttacaa aaaaattcaa aaaatgctgg
     5641 tttaatatct gcataataac taattttgta tgcttctttg ctcgttctcc tccttcccaa
     5701 agaaatagat tataagtcca tgggaagaag gtagatttat gaattcttta aaagaagatt
     5761 tcattttagc aaaggctgga aatgaagaag ctgttgaggc aattctcaaa agatttagct
     5821 ctttgattca taagcaatcc tggagaactg gaaaatatga tcaagattgt tatcaagaat
     5881 gtatgcttgc aatatatcta gctatctcga aattcgaaat taaagaataa ctcaactaag
     5941 ggagtctgtg acataagtaa aaagatagtt tcaaatttcg aacaataaat ttgaaactat
     6001 cttctttata attataaagt atcgatttta cagcgttcta taatgattca tagaatctaa
     6061 aacgaatgat tttaattatc aacgtttttt aattcgtttt tagcttaaaa ataacttttg
     6121 tcacagtctc actcctaaaa ttactttatt tacgtatgaa gtgggcagga ggtcgaatcc
     6181 agaactgggc tatggtactt caacgaggac ttctcctcta gaattgaaaa gcatgcgatt
     6241 taccttccgt aacgacggta gatttattca actatgtgtc caatcaaaat ttgtctgatt
     6301 tcaaaaatag tcaagagaat tttggttgtt aaaaactctt atctatcaac ttccacagtt
     6361 tagttgacaa accctgatac taagttgggc tatgatacac ttttcttgac tatcagttac
     6421 gttaccggaa ggaatgaact ctattgagaa aaacatatga tcgtaaattt aaactaaaga
     6481 tttcacagga tatccttgag aaaaaagtaa ctaccaagaa aattgctgaa gaatacaata
     6541 tctctcgtcc aacaatttcc cgatgggttt cagaatatcg tcgatacggg aaaaatgctt
     6601 ttgctggaca aggaaaaaga ttacctgata aagcagattt ctacattctt gaacaggaaa
     6661 ataaacgact aaaagaggaa gacgagtgtt ctacgaacac aaagatcgat acggatctcc
     6721 agggatcact caaaaacttt acgatgaagg aatcgaaatc aataaacgtg ttgtagctgt
     6781 tcttatgcgt gaaatgaatt tatgcacaag aggatttcat cgacgcaaat cttctcatgg
     6841 tagagaaaaa acaatcaaag aaatggtaaa aaaatgttga atcgataatt tgaacagaac
     6901 caaattgatg ctgtttgggt gatggatatt acttacattc catgtaatga cggtcgcctc
     6961 tatttgtcca cctatattga cttagcaact cgtattctac gttactatat ggtagattca
     7021 cacatgaaaa aagagattgt cgtccgtcca ttacaaattt attaaggaga attgcctacg
     7081 gtaattcatt ccgatagagg tagccagcat cggtctttta tttatcaaga gctgttgaac
     7141 gaccaccaca tcacacattc agtgagccaa actgggactc ctgttgacaa tgcagtaatt
     7201 gaagcgtttc atcgatcaat caaatgagaa ttaatcgact ccaacaaata taaaagtaaa
     7261 atagagatga aggtgttaat ccaagattat ttagaagact actacccaaa caaaagaatc
     7321 catacaaaat ttatgatgac tccttgacaa tttgaagcga agaaaataa
//
LOCUS       DACEEZ010000111         7046 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20313_2017
            SAMN12639966-rid9879223.denovo.111, whole genome shotgun sequence.
ACCESSION   DACEEZ010000111 DACEEZ010000000
VERSION     DACEEZ010000111.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639966
            Sequence Read Archive: SRR10023158
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7046)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7046)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741683.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741683.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:44:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,016
            CDSs (total)                      :: 2,962
            Genes (coding)                    :: 2,889
            CDSs (with protein)               :: 2,889
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 36 of 73
            Pseudo Genes (incomplete)         :: 29 of 73
            Pseudo Genes (internal stop)      :: 18 of 73
            Pseudo Genes (multiple problems)  :: 8 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7046
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639966-rid9879223.denovo.111"
                     /isolate="E20313_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            94..1248
                     /locus_tag="IWO89_002571"
     CDS             94..1248
                     /locus_tag="IWO89_002571"
                     /EC_number="2.3.3.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA synthase"
                     /protein_id="HAQ0457274.1"
                     /translation="MKIGIDRLSFFIPNLYLDMTELAESRGDDPAKYHIGIGQDQMAV
                     NRANEDIITLGANAASKIVTEKDRELIDMVIVGTESGIDHSKASAVIIHHLLKIQSFA
                     RSFEVKEACYGGTAALHMAKEYVKNHPERKVLVIASDIARYGLASGGEVTQGVGAVAM
                     MITQNPRILSIEDDSVFLTEDIYDFWRPDYSEFPVVDGPLSNSTYIESFQKVWNRHKE
                     LSGRGLEDYQAIAFHIPYTKMGKKALQSVLDQTDEENQERLMARYEESIRYSRRIGNL
                     YTGSLYLGLTSLLENSKSLQPGDRIGLFSYGSGAVSEFFTGYLEENYQEYLFAQSHQE
                     MLDSRTRITVDEYETIFSETLPEHGECAEYTSDVPFSITKIENDIRYYKI"
     gene            complement(1270..2022)
                     /locus_tag="IWO89_002572"
     CDS             complement(1270..2022)
                     /locus_tag="IWO89_002572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HAQ0457275.1"
                     /translation="MNEKEWDEFAEEYYEIQQESQVTIAEDVKNYLKTEKVLPAETAA
                     DVAGGSGRFLSLAKEINRYDLIDFSSGMLENAKKEAEKNELPNVSFIKQSLEEFIQSN
                     ETYEMVFTAANPALVHLIQLEKLMKKSSKWCVIIRVVESIDDVFYPIEKQLGMIEEDP
                     NTSPVVMDQFETYLIDEGFKVKKKDFTYFTQESITEELMKLYYDAYAKDPVVTRYIRK
                     LFSEKREILSTTKLTYRLLLWSADKKRTFHDS"
     gene            complement(2386..5052)
                     /locus_tag="IWO89_002573"
     CDS             complement(2386..5052)
                     /locus_tag="IWO89_002573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HAQ0457276.1"
                     /translation="MENKEQAQQAVYKETIDSLMQQTQSSTDGLSTTQAKEKLERDGL
                     NQLKEKPKKPTWKLFLETFKDAMVIVLLIVALIQMVMGDYIEFLVIFAVLMLNSIISV
                     VQTKKAEGSLEALKNLSAPDARVIRDGKEKTIPANELVVGDIVLLDAGDYVPADGRLL
                     DAGSLKVDEGMLTGESTSAEKEVTDITSTVPIGDRTNMVFSGTIVTYGRGKFLVTSTG
                     NNTEIGQVAGLLESTTEQVTPLQRGLDKFSKKLSLAILALSLLILGIQLFRIYLGEGT
                     GDMTADIVSAVMFAVAVAVAAIPEALQSIVTIVLSLGTNKMAKRHAIIRKLPAVETLG
                     STSIICTDKTGTLTQNKMTVVDYFLGNGDTGDFTNDPSKWTADERRLIEISVLANDAS
                     ISEDGTKLGDPTEVAFIDFSEKLNQPYQEIRNNYPRQAELPFDSDRKLMSTGHTIDNK
                     NYLFVKGGPDIVFQRSKKVLLNGEVVPMTDELMKKFQEQNEAFSTRALRVLAFAYRPL
                     DNFDLSFEDEDDLILVGLLAMIDPPRAEVTQAVTEARSAGIKTIMITGDHKTTAVAIA
                     KQIGIFEEGNLALTGTELDELTEDQLNEVLEKVTVYARVSPENKIRIVRAWQNKGHIS
                     AMTGDGVNDAPALKQADIGIAMGTGTDVAKDAAAMVLTDDNFASIVSAVEVGRNVFDN
                     IKKAISYLFAGNLGAIIAIIFALVADWSNPFTALQLLFINLVNDSLPAIALGTEKAEP
                     ATMNRPPRDPNSGIFTDKTLISVTYRGVLIGIAVIFAQWLGMQHSPEFGVAMSFSTLI
                     WCRTLQTFPARSNTQTAIQAGFFSNRSVLLAVVVCSALYCLTMIPGLKEVFSIPSAFG
                     LTEVFTCMGIALVTIVLMEITKLIIVRVQGNK"
     gene            5393..6844
                     /gene="cls"
                     /locus_tag="IWO89_002574"
     CDS             5393..6844
                     /gene="cls"
                     /locus_tag="IWO89_002574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase"
                     /protein_id="HAQ0457277.1"
                     /translation="MVSSIITALYLLNALIALVAILIKPRDVAAIWAWLLVLFALPGV
                     GFVLYLFFGRGLTDKKKFYLRQSDLKELENFQSFRGDTIEHYDPDMGDKDKQQFVDFF
                     SSLNRMPLTRMNSVTLLTDGQEKLDSLLQDLKKAKHSIHIEYYAFVTDNIGQQVLHVL
                     EEKAAEGVEVRILYDAFGSHGTKAKDFNRLIKNGGHVHTFVTSQRALLRFRLNYHDHR
                     KIVVIDGKISYTGGFNIANQYVNTTKKFGYWRDTHIRIFGAASSLLQLRFLTDWNVSV
                     PEEKKVGYHLNYFFKKADRDESKLADTSIQLVSSGPNNEREQIKLSFIKLITSAKKRV
                     WIQTPYLVPDESVIAALKIATASGVDVKIMIPNKPDHPFIYRATQYYARQLIKENVQI
                     LVYENGFLHAKTLIMDDEICMVGSANQDIRSYRLNFETSAVIYDPEFLEELATQFKED
                     ETHCSSMTTETVKEMSNWLLFKQQISRLFSPIL"
BASE COUNT         2072 a         1455 c         1277 g         2242 t
ORIGIN      
        1 aatccttaaa tattctcttt tttatatttc ttttaatctt tttatgatac cttagtaaag
       61 taagtttttt atcataagta aagaggtgtt gaaatgaaaa tagggattga tcgtctttcc
      121 ttttttattc ctaatttata tttagatatg acagagttgg cagaaagccg tggggatgat
      181 cctgcaaaat accatatagg gattggtcaa gaccaaatgg cagtcaatcg tgcaaatgaa
      241 gacatcatta cactaggagc aaatgctgcc agcaaaatcg taacagaaaa agaccgtgag
      301 ctaatcgaca tggtcatcgt cggaacagaa tccgggattg atcattcaaa agcgagtgcg
      361 gtaattatcc accatttact gaaaatccaa tcttttgctc gttcttttga agtaaaagag
      421 gcttgctacg gtggcaccgc agctttgcac atggcgaaag aatatgtcaa aaatcatcca
      481 gaacgaaaag tactagtcat agcaagtgat attgctcgtt acggcttggc aagcggtggt
      541 gaagtgacgc aaggtgtcgg tgctgttgcg atgatgatca ctcaaaaccc gcgtatttta
      601 tcgattgaag acgacagcgt atttctgaca gaagatatct atgatttctg gcgtccagat
      661 tatagcgaat ttcctgttgt tgatggtcct ttatctaatt ctacgtatat cgaatcattc
      721 caaaaagttt ggaatcgaca taaagaattg tcgggtcgag gactcgaaga ttatcaggcg
      781 attgctttcc atattccgta tactaagatg ggaaaaaagg cattgcaaag cgtattagac
      841 caaacagacg aagaaaatca ggaacgtctt atggctcgct atgaagaaag catccgttac
      901 agccgacgaa tcggtaatct ttacactggt tcattatacc tggggctaac ttctctactg
      961 gaaaattcga aatcactaca gccaggagat cgcatcggtc tattcagcta cggttctggt
     1021 gcagtaagtg aattttttac aggttatctg gaagaaaact atcaagaata tctcttcgca
     1081 caatctcatc aagagatgct agattctcga acacggatca ctgttgacga atatgaaacg
     1141 attttcagtg agacgcttcc tgaacacgga gaatgtgcag aatatacttc agatgtacct
     1201 ttttcgatta caaagatcga aaatgatatt cgttactaca aaatataata aaatcataaa
     1261 aagaaccctt cacgagtcgt gaaaggttct ttttttatct gcggaccaaa ggagcagtct
     1321 ataggtcagc ttagtcgtac taaggatttc tcgtttttca ctgaatagtt tgcgaatgta
     1381 cctggtcaca acaggatctt ttgcgtacgc gtcatagtat agcttcatca attcttcagt
     1441 aattgattcc tgtgtaaaat aagtaaaatc ttttttcttc actttgaatc cttcatcaat
     1501 taagtatgtt tcaaattgat ccattacaac tggcgatgta ttaggatctt cttctatcat
     1561 tcctagttgc ttttcgatag gataaaaaac atcatcgatt gattccacta ctctgattat
     1621 cacgcaccat ttgcttgact ttttcatcaa tttttctaat tgaataagat gtaccaatgc
     1681 tggatttgct gctgtaaaaa ccatttcata tgtctcattt gattggataa attcttctaa
     1741 cgactgtttg ataaaggaaa catttggcaa ctcatttttt tctgcttctt tttttgcatt
     1801 ttctagcatt cctgaagaaa aatcgatcaa atcatagcga ttaatttctt ttgctaagga
     1861 aagaaatctg ccagatcctc cagcaacgtc tgctgctgtt tcagcaggca ggactttctc
     1921 cgttttcaga taatttttta catcttcagc aatagttact tgcgattctt gctggatttc
     1981 ataatattct tcagcaaatt catcccattc tttttcattc atttgatttc ctctaatttt
     2041 ttgttatatg ttttcacttt tcggaataaa tcattctcct cttttatcta ttctatctct
     2101 tttgatgaat aataaaatga ttggctttcc gtctatcata aataaaaaaa gggactgtga
     2161 ccaaagtcgt gtcaaagtcc ctctttccga ataaacagtg tccaatcaga tgaccctcag
     2221 tattagctca aaatgctgat tttacaactt cttccttaaa cggtgttctc ctgccaactc
     2281 ttcggtcaca agtcacaata ttcaacaaac atgagaagct gtttgttgaa attgcacctt
     2341 gtgactcaga attaaacggc aggctcacaa cctcgttgat tcgttttatt tatttccttg
     2401 tactcgtaca atgatcaatt tagtgatttc catcaaaaca attgtcacaa gtgcgattcc
     2461 catacatgta aagacttctg tcaatccaaa tgctgatggg atagagaaga cttctttcag
     2521 tcctgggatc attgttaagc agtaaagcgc gctacataca actacagcta gtaagacact
     2581 tctgttagag aagaaccctg cttggatagc tgtttgtgtg tttgaacgtg ctgggaatgt
     2641 ttgcaatgtc cgacaccaaa tcaatgttga aaatgacatg gctacaccga attcaggtga
     2701 atgctgcatg ccaagccatt gagcaaagat aacagcaatc ccaatcagca caccacgata
     2761 cgttacagaa atcaatgttt tgtccgtaaa gattcctgaa tttggatctc ttggcgggcg
     2821 gttcatcgta gcaggttcag ctttttctgt acctaatgca attgctggca gtgaatcatt
     2881 gactaaattg atgaacaaca gttgtaatgc tgtaaatgga ttggaccagt cagcaactaa
     2941 ggcaaagatg atcgcaatga tcgcacctaa atttcctgca aatagataag aaatagcttt
     3001 cttgatatta tcaaacacat tacgtcctac ttctactgcg ctgacaatag aagcaaagtt
     3061 atcatccgtc aataccatcg ctgctgcatc ttttgccaca tctgtacccg ttcccatagc
     3121 aattccgatg tctgcttgtt tcaatgctgg tgcgtcattt acaccatcac ctgtcatagc
     3181 tgagatatga cctttatttt gccaagcacg aacgatacga attttattct caggtgaaac
     3241 acgtgcataa acagtgactt tttctaaaac ttcgtttagt tgatcttctg tcaattcatc
     3301 tagttccgtc ccggtcaaag ccaggtttcc ttcttcaaaa atcccgattt gttttgcgat
     3361 agcaacagct gttgttttgt ggtcaccagt aatcatgatt gttttgattc ctgcacttct
     3421 tgcttctgtt accgcctgag tcacttcagc acgtggagga tcgatcatag caagcaaacc
     3481 aacaaggatc aaatcgtctt catcttcaaa tgataaatca aaattatcaa gtggacggta
     3541 agcaaaagct agtacacgta atgcgcgtgt tgaaaaagct tcattttgct cttggaattt
     3601 tttcatcaat tcatccgtca tcggaacgac ttcaccattt agcaatactt ttttactgcg
     3661 ttggaagacg atatctggtc cgcctttaac aaacaagtaa tttttgttat caattgtgtg
     3721 tccagtagac attaattttc gatctgagtc aaaaggaagt tctgcttgtc ttgggtagtt
     3781 gtttcggatt tcctgataag gctggtttaa tttttcacta aagtcgataa acgctacttc
     3841 cgtagggtcg cccaatttag taccgtcttc actgatcgat gcatcattcg ctaaaacact
     3901 gatttcgatc aagcgacgtt cgtcagctgt ccattttgaa gggtcatttg tgaaatcccc
     3961 agtatcgcca ttacctaaaa agtagtcaac aactgtcatt ttgttttgtg tcaatgtacc
     4021 agtcttatct gtacagatga tacttgtcga accaagtgtt tcaactgctg gaagcttccg
     4081 aataatggca tgacgttttg ccattttatt cgttcccaaa gataacacga ttgttacgat
     4141 agactgtaat gcttctggaa tagcagctac ggctacggct acggcaaaca tcactgcgct
     4201 gacgatatct gcagtcatat ctcctgtacc ttctccaaga taaatacgaa acagttggat
     4261 acctaaaatc aataaagaca atgccaaaat agccaaactt agttttttac tgaatttatc
     4321 cagacctcgt tgtaacggag tgacttgctc agttgttgat tccaaaaggc cagcaacttg
     4381 tccgatctcc gtattattcc cagttgaagt cactaagaat ttaccgcgtc cgtaagttac
     4441 gatcgttcca ctgaatacca tatttgtacg atctccgatt ggtaccgtac ttgtgatatc
     4501 cgttacttct ttttctgcag aagtcgactc tcctgtcagc ataccttcat ctacttttaa
     4561 tgatcccgca tctagtaaac gtccatcagc aggtacataa tcgcctgcgt ctaacaacac
     4621 gatatctcct accacaagtt cgtttgccgg aatcgttttt tccttaccat cacgaatcac
     4681 tcgagcatcg ggcgctgaaa ggtttttcaa cgcttctaaa gaaccttctg ctttttttgt
     4741 ttggacaaca ctgattatag aattcaacat caatactgca aaaataacta agaattcgat
     4801 gtagtcaccc atgaccatct gaatcagtgc gacaatcagc aatacgataa ccattgcatc
     4861 tttgaaagtt tctaagaaca atttccaagt tggcttcttc ggcttttctt ttagctggtt
     4921 caagccatct ctttctaatt tttccttcgc ttgcgtggtg ctcaatccat cggttgagct
     4981 ctgcgtttgc tgcatcaacg agtcgatcgt ttctttatag accgcttgtt gtgcttgttc
     5041 cttgttttcc aaatttcatc ccccattttt tgatcttggc cctagtcctg tctgaaaaaa
     5101 aagagaccta agacaaatac gcatttgtct taggtctcac catttcatac aataccagaa
     5161 aaccatttgg ttaacttact gttgatatta tcgcaacaaa cgttgccggt tactccccta
     5221 aggagatatt caactgtact aatatcctac cttattaacc tacaaaaagc aactacttta
     5281 gcccgattta cctacatttt catgaaagga gtgaaaagta tattcacctt ggtaggcttc
     5341 tttcttctat catgctatac taatgttatt gtatgtaagg aggaacattt ttgtggtatc
     5401 tagtattata accgcccttt atctattaaa tgcacttatt gctttggtgg ctattttgat
     5461 caaaccccga gatgtagcag ccatttgggc atggctctta gtattgtttg cccttcctgg
     5521 ggtgggcttt gtattatatt tatttttcgg acgtggatta acggataaga aaaaattcta
     5581 tctccgacaa agtgacttga aagaattaga aaactttcag tcttttagag gagataccat
     5641 tgaacattac gatcctgaca tgggcgataa agacaaacag caatttgttg acttcttctc
     5701 ttcattaaat cgtatgccgc tgacaagaat gaattctgtc actcttctca cagacggaca
     5761 agagaaattg gattcactgc ttcaagatct aaaaaaagcc aaacattcga tccatatcga
     5821 atattacgca tttgtgacag ataatatcgg ccagcaagtc ttacatgttt tagaagaaaa
     5881 agccgcagaa ggcgtggaag ttcgaatatt atatgatgca tttggctctc atggcacaaa
     5941 agcaaaagat ttcaatcgtc taatcaaaaa tggtggacat gtccatacat ttgttacctc
     6001 acaaagggca ttacttcgtt tccgattgaa ttaccatgat caccgaaaaa tcgttgtgat
     6061 cgatggaaag attagttata ccggtggttt caatattgcc aatcaatatg taaatacaac
     6121 aaaaaaattc ggctattggc gcgatacgca tatacggatt ttcggtgccg cttcttcttt
     6181 gctccagctt cgcttcttaa cagactggaa cgtctcggta cctgaagaaa aaaaggtcgg
     6241 ctatcatttg aattatttct ttaaaaaagc agatcgagat gaatctaagc ttgctgatac
     6301 atccatccag cttgtttcaa gcggaccgaa taacgaaagg gaacaaatca agctttcatt
     6361 tatcaaattg attacttctg ctaaaaaacg tgtttggata cagacacctt accttgttcc
     6421 tgatgaaagt gtcattgctg ctttaaaaat cgcaactgcc tctggtgtag atgtgaaaat
     6481 catgattccc aacaaaccgg atcatccttt tatttatcga gcaacacaat attatgctcg
     6541 gcagctgatc aaggaaaatg tacaaatcct tgtctatgag aacggcttcc tccatgcaaa
     6601 aacattgata atggatgatg aaatctgcat ggtaggttca gcaaatcaag atattcgtag
     6661 ctaccgattg aattttgaaa caagtgctgt catttacgat cctgagtttt tagaagaact
     6721 tgctactcag ttcaaagaag atgagacaca ttgttcatcc atgacaactg aaacagtcaa
     6781 ggaaatgtct aactggctat tattcaagca acaaatttct cgattatttt ctccaatcct
     6841 ataatttttt tattcaagta gagaaaaata tactaagatt aaagggactg tgacaaaaat
     6901 cttgtcacag tcccttcttc cgaacaaacg gtgttctcct gccaactcct cggtcacaag
     6961 tcacaatatt caacaaacat gagaagctgt tttttgaact tgttccttgt gactcggagc
     7021 tggacggcag gctcacgacc tctttc
//
LOCUS       DACEEZ010000112         6643 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20313_2017
            SAMN12639966-rid9879223.denovo.112, whole genome shotgun sequence.
ACCESSION   DACEEZ010000112 DACEEZ010000000
VERSION     DACEEZ010000112.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639966
            Sequence Read Archive: SRR10023158
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6643)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6643)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741683.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741683.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:44:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,016
            CDSs (total)                      :: 2,962
            Genes (coding)                    :: 2,889
            CDSs (with protein)               :: 2,889
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 36 of 73
            Pseudo Genes (incomplete)         :: 29 of 73
            Pseudo Genes (internal stop)      :: 18 of 73
            Pseudo Genes (multiple problems)  :: 8 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6643
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639966-rid9879223.denovo.112"
                     /isolate="E20313_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(1..859)
                     /locus_tag="IWO89_002575"
                     /pseudo
     CDS             complement(1..859)
                     /locus_tag="IWO89_002575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611166.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
     gene            1102..1557
                     /gene="tnpA"
                     /locus_tag="IWO89_002576"
     CDS             1102..1557
                     /gene="tnpA"
                     /locus_tag="IWO89_002576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002366027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
                     /protein_id="HAQ0457278.1"
                     /translation="MSNDDKSLAYTRWNCKYHIIFTPKYQRKVIYGQLRKDLGKILRK
                     LCEMKDVEIIEAHAMLDHIHMLVRVPPKICISSFMGYLKGRSAVLIHEQHANLKYKYG
                     NKSFWSKRYYVSTVGLNQKTNEKYIREQEADDRIRYIISKREYVEPFKK"
     gene            1822..1920
                     /locus_tag="IWO89_002577"
                     /pseudo
     CDS             1822..1920
                     /locus_tag="IWO89_002577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339652.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
     gene            complement(2143..2372)
                     /locus_tag="IWO89_002578"
                     /pseudo
     CDS             complement(2143..2372)
                     /locus_tag="IWO89_002578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631988.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="resolvase"
     gene            complement(2602..3420)
                     /locus_tag="IWO89_002579"
     CDS             complement(2602..3420)
                     /locus_tag="IWO89_002579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HAQ0457279.1"
                     /translation="MSYIYLIKENYPFLTKSEKKVADFILNSGKTIIYSTMSDIKKNT
                     KVGDATIIRFCQKIGFSGFSDLKIEIAKEDFSQKKEHPTSVKYYDEIANSLTEALYST
                     IRLLDEEKLNEAIKMITQSKNIYIFGVGSSGNTSLDLENMFLRVGVQAKAVLDPHFQA
                     QVASLLTVNDLVIIFSLSGKTKDTYDSLKIAKKNGAKIIAITNYIHSPIGKSADLVLQ
                     TAIEEFLNGGSLAGKISQLYICDLLVHGYEQNNNIDSVELREKVLRSIIDKSIE"
     gene            3581..4270
                     /locus_tag="IWO89_002580"
     CDS             3581..4270
                     /locus_tag="IWO89_002580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine-6-phosphate 2-epimerase"
                     /protein_id="HAQ0457280.1"
                     /translation="MDKKRFLDLISKGLIVSCQALPGEPFYKEEESLMPYFALAAQRA
                     GAVGIRANSVRDIIEIRKCVSLPMIGIIKRDYPPESPFITATMREVDELVETGVEVIA
                     LDCTLRNRHDGLSINSFITKIKEKYPHQLFMADISTFEEGKNAYKAGIDFVGTTLSGY
                     TEESKKQDGPDIDLIKKLVEEGICVIAEGRIHTPEQAKEIKKIGVAGIVVGGAITRPQ
                     EIASRFVKALS"
     gene            4284..5786
                     /locus_tag="IWO89_002581"
     CDS             4284..5786
                     /locus_tag="IWO89_002581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010759843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAQ0457281.1"
                     /translation="MFKKFSQLGRAFMLPIAILPVAGLLLGLGGALTNESAINAYPFL
                     DQPWLHTILSIMSYAGNAVFANLALIFAIGIAVGLANGDKGTAGLAGGVAYLVYTATI
                     SGFLELFSAKDATLDTGVVGSIVVGGTVAFLHNRYRKIELPQFLGFFGGSRFIPIVSS
                     LAAIIIGSFFYLIWPPIQNGLVVAGEQIAQMGSLGTFLYGFLLRLTGAVGLHHTIYPL
                     FWYTSLGGTETVAGSTIAGAQNIFFAQLADPNHTGLFTYGTRFFAGRFATMMFGLPAA
                     CYAMYRAIPKKNRKKNGGLYFSGALTSFLTGITEPVEYMFLFVAPWLYVIHAFLDGLS
                     FYFADILNIRVGNSFSGGLIDFLLFGVLQGNDKTNWLKLIPFGIAWAFIYFFVFTFFI
                     KKFKVAIPGMEEESEVHSEKVINKNTSLHEESLIIIEALGGKENIESVEACATRLRVA
                     VTNGEIIQKQTIKDTGATAVFEVKGGIQAVFGGKADLLSQEINQILGKDN"
     gene            5799..6275
                     /locus_tag="IWO89_002582"
     CDS             5799..6275
                     /locus_tag="IWO89_002582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HAQ0457282.1"
                     /translation="MFEIFKKKKQETFYAPCSGKIISITEVNDPVFSQKLLGDGFAVI
                     PDGSKIYAPLAGRVTNIFPTKHAINIKSNSGIDYLIHIGIDTVELQGEPFSICVQEND
                     EVTPETSLVDVDFDKIIKSGKDTSVIVVFPEKNQFNDLIIDNGRIAHGNSCGKITF"
     gene            complement(6372..6482)
                     /locus_tag="IWO89_002583"
                     /pseudo
     CDS             complement(6372..6482)
                     /locus_tag="IWO89_002583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252604.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT         2171 a         1032 c         1204 g         2236 t
ORIGIN      
        1 acttgtcctc gataagaatt gtagcgccgt gatttacgtg tgtatgtcgt tacttttatt
       61 cctagcttct tgatcaggcg ctgaactttc tttttattga tagagaaacc gcggtttctt
      121 agttcattgg tcatccgtaa acagccataa tccttgtgct tctcccgaac tttaagcatt
      181 tccgcttcaa tctcttgatc tggattttta cggtccaatc gtttttgcca gtacataaag
      241 attgccttag ggaactttaa agtttctaga agctcaacta atttaaacgg tcctcggagg
      301 cttgcgatga ttcgtgcgat tgcttcattc gtcgttgttg ggcttcctgt tttcgcagct
      361 tcctcaattc ttttaaaaag gcattttcaa tttgaaggga acggactcgt ttctccaact
      421 cctcgatgcg ttcacgttct ttagacgttg ctttagttgt agattttttc ttatttttgg
      481 gttcattttt cttagacaag ataggtggac atcctttcgt ttttgagagt ccatcgatcc
      541 caccctcacg gaattgacgc atccaactag cgatcaaatt agggttgttc atgttaagtg
      601 tgttggcaac ttcacgatag gacagctcac ttgttagata taactctatc gcatccagct
      661 taaattgaac agaatatttt ttcttttgtc gctttcttac tagaccctct tcaccaaatt
      721 cttgataggc attgatccag cgcttcactt gtgatggagc cttaatggaa tatttctttg
      781 ccagataatc acttccgcct ttaccatcca agtaatcatg aacgatcttt aatttaagtt
      841 caaaagtata cttagccata aaaaataccg acctccaaaa gttagatttt ttggtctaac
      901 ttttgggggt cggtacaaaa cttaaaagtt ttagaccctt ttattttctt aatgacttca
      961 gtcagttttg tgcgtaaagc gtgcgaaaca agaaaaccgc gcgtttctac gcgtggaaga
     1021 agtatcttta gcgataaaaa ctcctttcga tatactagtg agtggctacc aaccgctcat
     1081 cagatcgaaa ggagtttttt catgtcaaat gacgataaaa gtttagcata tactcgttgg
     1141 aattgtaagt atcatattat ttttacacca aaatatcaac gaaaagtaat ctatgggcaa
     1201 ttacgaaaag atcttggaaa aatacttagg aagttgtgcg aaatgaaaga tgtggaaatc
     1261 atagaagcac atgcaatgct agatcatatt cacatgcttg ttcgagtacc accaaaaata
     1321 tgtatatcca gttttatggg gtatctgaaa ggtcgtagtg cggtactaat ccacgagcag
     1381 cacgcaaatc tcaaatataa atacggaaac aaatcctttt ggtcgaagag atactatgtg
     1441 agtacagtcg gattgaatca aaagacaaat gaaaaataca tccgtgaaca agaagctgat
     1501 gatcgcattc gatatattat aagtaaaaga gagtacgtag aaccatttaa aaaataaaga
     1561 gaagtaagtg agtgaagatg agcagttggc ggtcttttaa aaatcccctt ttaaggggca
     1621 gcgagtgtcg accccttcta gggggattaa aaatacaccc gtcctcacgg gtgtatgggt
     1681 tactgataga cttgatttta actggtgtca gaaacttatg tccagaaagg gtgatgaaat
     1741 tttggcaatt tacggctatt caagaattag cacaagcaac caagattaca aaacacaaat
     1801 tcaaacatta gaaaatgctg gcgcagaaat aatcttccta gaaaatcact ggattatcga
     1861 acacctttgg aagtattttt gagttacgta agtattgatg atctgtctaa cttaatttga
     1921 caattaagag tatatttact tcagatggta aggcaacaaa ataaaataca ataggcagat
     1981 gaactaataa ataaaatctg tattaaatac agataaatta atataaaaat caaaaggatc
     2041 ataaactttt aacaagtgat gaaatgggtg gaaattgatt cttatagaca gtttgtctca
     2101 taacattgaa gtattttgca attttagcat tgaaacactt ttttaatctt caacgttgtt
     2161 cttataaaca agatgattgg gaatcttttg aattaacact gtatagctta gaacaccctt
     2221 tatagaaaac attcgcttag caatcttaat aattccttgt gcccgatgtc gtttagattc
     2281 agtaggttcc tgctcatcga tcatggaaaa atttgaagga tcagatcctt aataaattta
     2341 tttaagagag gatgatccat tgcttcatcc atactcgata aactagtgat aatgagctac
     2401 acgtttttct ttcaagttga tcaatagctt ccacgataaa aatatcttta gataaaatca
     2461 agaaccgtag gaactacagg gatatttggt caataagagc caccgctatt agtaaaaaat
     2521 aagctatcaa gtatgctctc atcccaagaa ttgcctactt caatcgttaa aaaccgttag
     2581 gtttagctaa gtgggggtag ttcactcaat acttttatca ataatagagc gcaaaacttt
     2641 ttctcttaat tcaactgaat caatattatt gttttgttca taaccatgaa ctaatagatc
     2701 acaaatataa agttgtgata tttttccagc taaagatcca ccattaagaa attcttcaat
     2761 ggctgtttgt aaaactaagt ctgcagactt tcctataggt gaatgaatat agttagttat
     2821 agcaataatt tttgcgccat tttttttagc tattttcaat gaatcataag tatctttcgt
     2881 tttcccagat aaagaaaaaa tgattactaa atcattaact gttaataaag aagcgacttg
     2941 ggcttgaaaa tgagggtcta gtacagcttt tgcttgaact cctacgcgaa gaaacatatt
     3001 ttctaaatca agactagtat ttccactaga tcccactcca aatatatata tgttttttga
     3061 ttgagtgatc attttaatgg cttcattcaa tttttcttcg tctaaaagcc taatagtgga
     3121 atagagagct tctgttaaac tattagcaat ttcatcatag tactttacac tagtcggatg
     3181 ttcttttttt tgactaaagt cttcctttgc aatttcaatt tttaaatcag aaaaaccaga
     3241 aaaaccaatt ttttgacaaa agcgaataat agtagcatcg ccaacttttg tgtttttctt
     3301 aatatcactc atcgtactat aaataatagt tttcccagaa tttaaaataa aatcagcgac
     3361 ttttttttca gacttcgtta aaaatggata attttcttta attaaataga tataactcat
     3421 acaaactcct ctacaatttc aacttttctc cattatactc caaaataata aaaaaaactc
     3481 ctaaatactc catttttatc tttactttta tgaaagcgga tgctatattg tttttggagt
     3541 tgttatccaa aaataataaa aaattggagt gtttaggata atggataaaa agaggttttt
     3601 agatcttatt tcaaaaggtt tgattgtttc ctgtcaagca ttacctggtg aaccatttta
     3661 caaagaggaa gaaagtttaa tgccttattt tgctttggct gctcaaaggg ctggagcagt
     3721 tggtatacgt gcaaattctg ttcgagatat tattgaaatt agaaagtgtg tatcattacc
     3781 aatgattgga attattaaaa gagattatcc accagaatct ccattcatta ctgccactat
     3841 gagagaagta gatgaattag tagaaaccgg tgttgaggta atcgctcttg attgcactct
     3901 tagaaatcga catgatggac tatctataaa ttcttttatt actaaaataa aagaaaaata
     3961 tcctcatcag ttatttatgg cagatatttc tacatttgaa gaaggcaaaa atgcatataa
     4021 agcaggaata gattttgtag gaactacttt aagtggatat actgaggaaa gtaaaaaaca
     4081 agatgggcca gatatagatt tgattaaaaa attggtagaa gaagggattt gtgtaattgc
     4141 tgagggaagg attcatactc cagaacaagc aaaagagata aaaaaaatag gagttgcagg
     4201 tattgttgtg ggtggtgcta ttactcgacc acaggaaatt gcatcaagat ttgttaaagc
     4261 attaagttag ggggagaaat aaaatgttta aaaaattttc acaattagga cgcgctttca
     4321 tgttaccaat tgctatatta ccagtagccg gattactatt gggactggga ggagcgttaa
     4381 caaatgagag tgctataaat gcttatccat ttttagatca accgtggttg catacaattt
     4441 taagtattat gagttatgct ggaaatgcag tttttgcaaa cctagcacta atttttgcaa
     4501 ttggtattgc tgtaggactt gcaaatggag ataaaggaac agctggttta gctggtggag
     4561 tagcttatct agtatatact gcaactatta gtgggtttct tgaacttttt tcagcaaaag
     4621 atgcaacact agatacaggt gtagtgggat caattgttgt tggtggtact gttgcatttt
     4681 tacacaatcg ttatagaaaa attgaattac cacaattctt aggttttttt ggaggatcaa
     4741 gatttattcc gattgtttct agtttagctg caatcattat tggttctttc ttttatttaa
     4801 tttggccacc aattcaaaat ggtttagtag ttgctggtga acaaattgcc caaatgggta
     4861 gtttaggaac ttttttatat ggttttttat tgcgtttaac aggtgctgtt ggactacacc
     4921 acaccattta ccctttgttc tggtatacat ctttaggtgg taccgaaact gtagctggat
     4981 caacaattgc tggagcccaa aatattttct ttgcccaact agctgatcca aaccacactg
     5041 gtctgtttac ttatggtact agattttttg caggaagatt tgcaacaatg atgtttggat
     5101 taccagcagc atgttatgca atgtatcgtg caattcctaa gaaaaatcgt aaaaagaatg
     5161 gtgggcttta ttttagcggg gcccttacat catttttaac tggaattact gaaccagttg
     5221 aatatatgtt tttatttgtt gctccttggc tttatgtgat tcatgcattc ttagatggat
     5281 tatcttttta ttttgctgat atcttaaata ttcgtgtagg gaattccttt tcaggtggtt
     5341 tgatcgactt tttattattc ggtgtacttc aagggaatga caaaactaat tggttaaaat
     5401 taattccttt tggtattgca tgggcattta tatatttctt tgtttttaca ttctttatca
     5461 aaaaatttaa ggtagctatt cctggaatgg aagaagaatc agaagttcat tctgaaaaag
     5521 taataaacaa aaatacatca ttacatgaag agtctcttat cattattgaa gctcttggtg
     5581 gaaaagaaaa tattgagtct gttgaagctt gtgcaactcg tttgcgtgtt gctgttacga
     5641 atggggagat tattcaaaaa caaacaataa aagacactgg tgcaactgct gtcttcgaag
     5701 tgaagggtgg catccaagct gtttttggag ggaaagctga cttattaagt caagaaatca
     5761 atcaaatatt aggaaaagat aattaggagg tttttaatat gtttgaaatt ttcaaaaaga
     5821 aaaaacaaga aactttttat gcaccttgtt ccggaaaaat catttccatc acagaagtaa
     5881 atgatcctgt cttttcacaa aaattactag gtgatggttt tgccgtcata cctgatggtt
     5941 caaaaattta tgctccattg gctggtcgag taacaaatat atttcctact aaacatgcta
     6001 ttaatataaa aagcaactca ggcattgatt atttgattca tattggaatt gatacagttg
     6061 aattacaagg agagcctttt tcgatttgtg ttcaagaaaa tgatgaggtt actcctgaaa
     6121 cctcgttggt tgatgtggat tttgacaaaa taataaaatc tggtaaagat acttctgtta
     6181 tagttgtttt tccggagaaa aatcagttca atgatttaat aatagataat ggtcgaattg
     6241 ctcatgggaa ttcatgtgga aaaatcactt tttaaaaaag tgtatataaa aagctataaa
     6301 ataagctaaa taggatattt gtcaaatgaa gttggtgaac agaaagaact gttttctttc
     6361 tgttcatttt tttattttat tagtaatgat tccccatgcc aaatcatgat ccgtaattta
     6421 aagttataaa agcttcgaaa tccattggca tttcgttgga ataccttgat atgattattc
     6481 aaatataaaa tatagagaga agagagagag ggaagatatt tataaagggc aaagttatgt
     6541 ttactcaact taggaaatag aaatagatca atttatattc tcttgtcaat cgaatatgaa
     6601 ccttagcact atataccaaa tatagtaaaa tgctgaaaga tgt
//
LOCUS       DACEEZ010000113         6570 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20313_2017
            SAMN12639966-rid9879223.denovo.113, whole genome shotgun sequence.
ACCESSION   DACEEZ010000113 DACEEZ010000000
VERSION     DACEEZ010000113.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639966
            Sequence Read Archive: SRR10023158
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6570)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6570)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741683.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741683.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:44:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,016
            CDSs (total)                      :: 2,962
            Genes (coding)                    :: 2,889
            CDSs (with protein)               :: 2,889
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 36 of 73
            Pseudo Genes (incomplete)         :: 29 of 73
            Pseudo Genes (internal stop)      :: 18 of 73
            Pseudo Genes (multiple problems)  :: 8 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6570
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639966-rid9879223.denovo.113"
                     /isolate="E20313_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            127..579
                     /locus_tag="IWO89_002584"
     CDS             127..579
                     /locus_tag="IWO89_002584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000751236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0457283.1"
                     /translation="MKKTILSIFSVFTILIGGYFLLNLDSVNSNAQIKDQRVAEHNNK
                     KMSLTKNIYSMSDEELYKEFGDLSGEQLEESIPGISDSDKIVVLSAGGQLMGDGKSEL
                     VSYKQNHRQFIDVNTDSNAISIKDLKELLNSKRNELKQIKETNSVELE"
     gene            593..1282
                     /locus_tag="IWO89_002585"
     CDS             593..1282
                     /locus_tag="IWO89_002585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000718009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0457284.1"
                     /translation="MKKIFGVSVGLLMMLTFGNAVYAAEKEVISDPEQYASRYFADFE
                     EEDKVLALPEGGFLKGEAKIVSMDNPDIIQGSYNSETDPASVSVTEAKKIVAEKFKND
                     IETTPIMPRGAFPPDVGTSGHLLVLAGGGSYKSQPFSGAGWRFGGMGVKPADGTGNFL
                     RWKSYIDDGRVGNVNQANATKSGSIQGMALYAREGFKYFAPNGPGWTMNIGIYYTYNP
                     VAGTYYVVQNI"
     gene            1407..3758
                     /locus_tag="IWO89_002586"
     CDS             1407..3758
                     /locus_tag="IWO89_002586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000344083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1906 domain-containing protein"
                     /protein_id="HAQ0457285.1"
                     /translation="MDEMVLSTQKWLNKKYSNVTGFDKVPENGRTGWPTIYGLIEGLQ
                     VELGITNLVANFGPTTEKMYDNQVTPKWGKNLPKNIVFLIQGAFWCKGINPGGFDGVY
                     TPYLDTAVKELQTDAGFNGSAVTGVLDAKWAKALFDMSAFVLVPGGDSKIRKMQQWLN
                     VNYLEYTGIMPCDGIYQRNSNQALIFALQAELGYSPSEATGSYGNGTTSKTYPVSEGN
                     SGNYVRIIQYGLYVNGYFEDGTFDGIFTKYMGLEVLAFRKFMVLPEYTEIADVVVIKG
                     LLSSAGDIRRSADGADTSTQLTRSQIQTLVDNDIKIVGRYLTGTVGIGKDERNKYLTT
                     EELNNIFSKNLSVFPIYQDGGAALAYFTYDRGLSDGKKAIDAAKNLNIPLTTVIYFAV
                     DLDMLGEEILAYAGEYFKGVSAVMSYSGYQTGVYGTRNVCSQIINNGYASFAFVSDMS
                     TGYSGNLGFPMPRQWSFDQFVEFTIGSGNGAVGIDLIATSGRDSGFNELSNDTNNDNY
                     IAKYNQKVINQMVRAYQYLSQAGLDNPWNPHLTFYRYVNYSGLSWDIISSPVTEHDRK
                     IYDEYRNKLTNSEGLYNYFIDPNTGSIIGLPHLIVTLQSQMFLADTINDSVSDFAGWL
                     GDLMTCWGEVKKLGIQMEQGVFALVGTAAGGTFSLEDLYQDVDAVNVYTLAKRNSDST
                     ALSALKTYYCDKGAIDRFNQFVKNRFGSSDKIASVTNSLLENDETVDILGVGIISFFI
                     DKIVNNSLGTDFTYLDFIGDEGKVANGFSEKIIQFQENRSKLN"
     gene            3838..4233
                     /locus_tag="IWO89_002587"
     CDS             3838..4233
                     /locus_tag="IWO89_002587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0457286.1"
                     /translation="MMIMEEIEWNNLTQNNKNNSGCGIIFMFVIVLGIVVLLITVKIV
                     DYFANTPEEQIQRKLPEFERVPPYDPRDNSFQVKKLKESPNEMWFEVTVESSDFKEKK
                     KEYNYIVKAKVSKRFIFNPKLEIYSVVIE"
     gene            4558..5175
                     /locus_tag="IWO89_002588"
     CDS             4558..5175
                     /locus_tag="IWO89_002588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001224538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HAQ0457287.1"
                     /translation="MRKFFYIRVSSYDQSIERQLISAKELGIPEEYIFVEKASGKDFK
                     RPEYQLMKRMFRKGDLLYLQSLDRLGRNKQMILEEWRELTKEKQIDISVLDMPLLDTA
                     SYKQLDGLETLISDLILQLLSYMAEDERKRINSRQREGVEAAKQKGVKFGRKKIALPP
                     EFPAIYSDWKAGKITAVYAMDQLGMKRNTFYRRVKEYEHTLHHFS"
     gene            5216..5515
                     /locus_tag="IWO89_002589"
     CDS             5216..5515
                     /locus_tag="IWO89_002589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000366408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0457288.1"
                     /translation="MDLTNQRVVFTGTLQQMTRTQAIGLVHSLNGHCQSSVTSKTNFL
                     VCGQYSKSLFDDSLYTKKEQTARDLIALGHEITILSEVEFYALISQQLKEWQRYL"
     gene            5549..5716
                     /locus_tag="IWO89_002590"
     CDS             5549..5716
                     /locus_tag="IWO89_002590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000824191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HAQ0457289.1"
                     /translation="MKRKSYDRWGIEITSFNRNIAFHVECDCGELAKSVYQKNYFYCP
                     KCHRRYGVHGT"
     gene            complement(5761..6366)
                     /locus_tag="IWO89_002591"
     CDS             complement(5761..6366)
                     /locus_tag="IWO89_002591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HAQ0457290.1"
                     /translation="MGKIGYIRVSSQDQNLDRQLAMMEGLNIDKLFQEKASGKDTERP
                     EFQKLLNYIREGDCVIVTSLDRLGRDYEDIKNTVAFMKQKKVALKILDAQFLDFNTGN
                     ELLDTAMFDMFLSLLSYIAQNEREKIRERQRQGVLLAQKAGRYKGRPIEYSINSTDPQ
                     KRLVFKTVVDMLKQGIPVAEIAKENGISRPTIYKIKKANDL"
BASE COUNT         2265 a          910 c         1285 g         2110 t
ORIGIN      
        1 attcctcctg ctatcttttt ctattattct atcttatttg atagtagatt taaaactttg
       61 caacagaacc aattatctta tcgtagatat atattataaa tttaataaga aagcgaggtg
      121 agaatcgtga aaaaaacgat actaagtatt ttttcagtat ttacaatatt aattgggggc
      181 tattttcttt tgaatttaga ttctgttaat tcaaacgctc aaataaaaga tcaaagagta
      241 gctgaacata ataataaaaa aatgagtttg accaaaaata tctattctat gtcagatgaa
      301 gaattgtata aagaatttgg tgatttatcc ggggaacagt tagaagaaag tattccagga
      361 attagtgact cagataaaat tgttgtcttg tctgctggtg ggcaattaat gggagatggc
      421 aaatccgaat tagttagtta taaacaaaat cacagacagt ttattgatgt taatactgat
      481 tccaacgcta tttcgattaa ggatctaaaa gagcttttaa atagtaaaag aaatgaatta
      541 aagcagataa aagaaactaa ttcagtggaa ttagagtaaa ggaagattca ttatgaaaaa
      601 gatttttggt gtttctgttg ggctacttat gatgttaaca tttggaaatg cagtatatgc
      661 tgctgagaaa gaagttattt cagatccaga gcagtacgcc tcacgatact ttgctgattt
      721 tgaagaagaa gataaagtac ttgcattgcc agaaggtggt tttttaaaag gggaagctaa
      781 aattgtatct atggataatc cagatataat tcaaggttca tataattcag aaactgatcc
      841 tgcttcagtt tcagtgacag aagcaaaaaa aattgttgct gaaaaattca aaaatgatat
      901 tgaaactact ccaattatgc ctcgaggagc atttccaccc gatgttggta ctagcggaca
      961 ccttcttgta ctagcaggag gaggaagcta taaatcacag ccatttagtg gtgcgggatg
     1021 gcgttttgga ggtatgggtg taaaaccagc tgatggtaca ggcaactttt tacgttggaa
     1081 atcctacatt gacgatggaa gagtaggaaa tgtaaatcaa gccaacgcaa ctaaatctgg
     1141 ttctattcaa ggaatggcat tgtatgctcg tgaaggattc aaatactttg caccaaacgg
     1201 gcctggttgg acaatgaata taggtattta ttacacttat aatcctgttg caggtacgta
     1261 ttatgttgtt caaaatattt aataaaacag cacaaagaga tttctttgtg ctgttttatt
     1321 atttttagtt gttgacaaac actaaaatat aaaatataat caacacataa acgaacgtat
     1381 gttcggcaag aaagcagggg atttttatgg atgagatggt tttaagtact cagaaatggc
     1441 taaacaagaa atacagtaat gttacagggt ttgataaggt tccagaaaat gggcgaacag
     1501 ggtggcctac aatctatgga cttattgaag gactacaagt agaacttggt attactaatc
     1561 tagtagctaa ttttggacct accactgaaa aaatgtacga taatcaggtt actcctaaat
     1621 ggggcaaaaa tctacctaaa aatattgtat ttcttattca aggtgccttt tggtgtaagg
     1681 ggattaatcc aggtggcttc gatggggtat atactccata tttagatact gctgtaaaag
     1741 agttgcagac tgatgcaggg tttaatggaa gcgctgtaac tggagttttg gatgcgaaat
     1801 gggccaaagc gttatttgat atgagtgctt ttgttcttgt tcccggcgga gatagtaaaa
     1861 ttagaaaaat gcagcaatgg ttaaatgtaa attatttaga atacacggga ataatgcctt
     1921 gtgatggtat ttatcaaaga aattcaaatc aagcattaat ttttgcatta caagctgaac
     1981 taggatattc tcccagtgaa gcaacagggt cttatggtaa tgggacaact agtaaaactt
     2041 atcctgtgtc tgaaggtaac tcgggaaact atgtaagaat aattcaatat ggattatatg
     2101 ttaatggtta ttttgaagac ggtacattcg atggtatttt cactaaatat atgggtttag
     2161 aagtactggc attccgaaaa tttatggtat tgcctgaata tactgaaata gcagatgttg
     2221 ttgtaataaa aggactattg tctagcgctg gagatattag aagatctgca gatggcgcag
     2281 atacttctac gcaattaact agatctcaaa tccaaacatt ggttgataat gatataaaaa
     2341 tagttggtag atatcttact ggtactgttg gaattggaaa agatgaacga aataagtatc
     2401 taactacaga ggaattgaat aatatttttt ctaaaaattt atctgtgttt cctatctatc
     2461 aagatggagg tgcagcatta gcttatttta cttatgatcg tggcttatca gatggtaaga
     2521 aggctataga tgcagcaaaa aatctaaata taccattaac aacagttatt tattttgctg
     2581 ttgatctaga catgttaggg gaagaaattc ttgcatatgc tggagaatat tttaaggggg
     2641 tatctgctgt aatgtcatat agcgggtatc aaacgggtgt ttacggaaca agaaatgttt
     2701 gttctcaaat aatcaataac gggtatgctt cgtttgcatt tgtttcagat atgtcgactg
     2761 gttatagtgg gaatttaggt tttcctatgc ctagacaatg gtcttttgat caatttgttg
     2821 aatttaccat aggaagtggc aacggagctg ttggtatcga tttaatcgct acatcgggta
     2881 gagatagcgg atttaatgag ttatcaaatg atactaataa tgataattac attgccaaat
     2941 acaatcagaa ggttataaat cagatggtac gtgcgtatca atatctatca caagctggtc
     3001 ttgataatcc ttggaaccct catttgacat tttatagata tgtaaattat tctggtttgt
     3061 catgggacat catttcttct cctgtaacag agcatgatag aaaaatttat gatgaatata
     3121 ggaataaatt aactaatagc gagggcctgt ataattattt tatagatcct aatactggtt
     3181 caataatagg tttgcctcac ttaattgtga cgttacaatc acagatgttt ctagccgaca
     3241 caatcaatga tagtgtttct gactttgcag gttggttagg agatttaatg acttgctggg
     3301 gtgaagttaa gaaattagga atacaaatgg agcaaggtgt atttgcatta gtaggtacgg
     3361 ctgcaggtgg aacatttagt ctagaagatt tataccaaga tgttgacgcg gtaaatgttt
     3421 ataccttagc aaaaagaaat tctgattcaa cagctttatc ggctttaaaa acgtactatt
     3481 gtgataaggg tgctattgat agatttaatc aatttgttaa gaacagattt ggttcttcag
     3541 ataaaatcgc ttcagtaaca aatagtttat tagaaaatga cgaaacagtt gatatcttag
     3601 gtgtagggat aatttcattt ttcattgata aaatagttaa taattcactt gggacagatt
     3661 ttacttatct tgattttatt ggagatgagg ggaaagttgc aaatggattt tctgaaaaaa
     3721 ttattcaatt tcaagaaaat agaagtaaac ttaattaaga atcgtttact atttaaacag
     3781 tgtccttttc ctttttaaat atatacgata aaataagtat atgaattgta aaaggacatg
     3841 atgattatgg aagaaataga atggaataat ctgactcaaa ataataaaaa taattctgga
     3901 tgtggaatta tctttatgtt tgttatagtt ttgggaatag tagtactttt aataactgtc
     3961 aagatagtag attactttgc gaatactcct gaagaacaaa tacagagaaa acttcctgag
     4021 tttgaaagag taccacctta tgatccaaga gataattcat ttcaagtaaa aaaattaaaa
     4081 gagtctccaa atgaaatgtg gtttgaagtt acagttgaat caagcgactt caaagaaaaa
     4141 aagaaagaat acaattatat tgtaaaggca aaagtatcaa aaagatttat ttttaaccct
     4201 aaattagaaa tatatagtgt tgtaatagaa taatagtgtt aaaaaaattg taaaagttaa
     4261 agaagtcaaa ttctgatgtg aaaaataaaa tataccatgg aagacataaa agaaaaactg
     4321 gataactagt ttatttaatg gcagaccgtt aaggcctgcc atttttatgt attttagcgg
     4381 aataaatata atcatgcggg agtgtaaact tactgtttca aaaagtatac tttttgacat
     4441 gccttgattt tctttgttta tgtatggcat aataaccaca taatacaatt tgaaacgttt
     4501 ctgatttaat ctgattgttt tgaaacaatt ttagtgaaaa aagggtagat aaaaacgatg
     4561 agaaaatttt tttatatccg ggtcagtagt tatgatcaat cgatcgaacg acaactgatt
     4621 tccgcgaagg aattaggaat ccctgaagaa tatatatttg tagaaaaagc cagcggcaaa
     4681 gattttaaac gccctgaata tcaattaatg aaacggatgt ttcgaaaagg agatttactc
     4741 tatctacaat cattagatag actaggcaga aataaacaaa tgattttaga agaatggcga
     4801 gaattaacta aggaaaagca gattgatatt tctgtgttag acatgcctct gttagatact
     4861 gctagttaca agcaactaga tgggttagag acgcttatct ctgacttaat tctgcaatta
     4921 ctcagctata tggccgaaga cgagcggaaa cgaattaata gccggcaaag agaaggcgta
     4981 gaagctgcga aacaaaaggg tgtaaaattc ggcagaaaga aaattgcttt acctcctgaa
     5041 tttcctgcga tctattctga ctggaaagca ggaaagataa ctgcagtcta tgctatggat
     5101 caattgggca tgaaacgaaa taccttttat cgacgggtga aagaatatga acataccttg
     5161 caccactttt cttgatagat ataacagcta ttagacggga agggagagtt tgaaaatgga
     5221 cttaacgaat caaagggtcg tttttactgg aacgctgcag caaatgacac gaacccaagc
     5281 catcggtttg gttcactctt taaacggcca ttgtcaatca agtgttactt caaaaacaaa
     5341 tttcctggtt tgtggtcagt attctaagtc gttgtttgat gattctctct atacaaaaaa
     5401 ggaacaaacc gcgcgggact tgattgcgtt aggtcatgag attaccattt tatcagaagt
     5461 cgaattttat gcgttaatta gtcaacagtt gaaagaatgg caacggtatt tataatcgtt
     5521 agtaaaattt acaaggagga aaatttttat gaagcgaaaa tcttatgatc gttggggcat
     5581 tgaaataact agttttaatc gaaatattgc ttttcacgtg gagtgtgact gtggggaatt
     5641 agcaaaaagt gtttatcaaa aaaattactt ttactgtcct aaatgccatc gccgatacgg
     5701 ggtacatgga acatagaaac gaaatagtgc ggtagaaaag cgaatgaatg tagtatattt
     5761 ctacaaatca ttcgctttct ttattttata aatagttggt cgagagatcc cgttttcttt
     5821 tgcaatctct gcaactggaa tgccttgctt taacatgtcc accacggttt taaaaactaa
     5881 gcgtttttgc ggatctgttg agttaataga atactcgatt ggtcgtccct tatatcggcc
     5941 ggctttttga gccaataata ctccttgccg ctgacgttca cgaattttct cacgctcgtt
     6001 ttgtgcaata taacttagca gtgataaaaa catatcgaac atagcggtat ccaggagttc
     6061 atttccagta ttaaaatcta aaaactgtgc atctaatatt tttaacgcta cttttttctg
     6121 tttcataaaa gcaactgtat tttttatatc ttcataatct cttcctaaac gatctagtga
     6181 tgtaacgatg acgcaatcac cttcacggat ataatttagt aatttttgaa actcaggacg
     6241 ctcagtatct ttaccactgg ctttttcttg gaataattta tcaatgttaa gtccttccat
     6301 cattgccaat tgtcgatcca aattttgatc ttgtgaagat actcgaatat agccaatttt
     6361 tcccataaaa aactcctttt taaaagtgat ataatttaca tgtaaaattt acttgtagtt
     6421 ttactgctat catagcattt ttaaaaataa attttatgta gacttaaaat ttacttcatg
     6481 gagggtttaa atggatgaaa tcaaaagtaa caaaaaagca tttggcattt atttaggaga
     6541 acctagtaca gaacaattaa accaattttt
//
LOCUS       DACEEZ010000114         6566 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20313_2017
            SAMN12639966-rid9879223.denovo.114, whole genome shotgun sequence.
ACCESSION   DACEEZ010000114 DACEEZ010000000
VERSION     DACEEZ010000114.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639966
            Sequence Read Archive: SRR10023158
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6566)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6566)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741683.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741683.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:44:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,016
            CDSs (total)                      :: 2,962
            Genes (coding)                    :: 2,889
            CDSs (with protein)               :: 2,889
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 36 of 73
            Pseudo Genes (incomplete)         :: 29 of 73
            Pseudo Genes (internal stop)      :: 18 of 73
            Pseudo Genes (multiple problems)  :: 8 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6566
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639966-rid9879223.denovo.114"
                     /isolate="E20313_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            <1..641
                     /locus_tag="IWO89_002592"
     CDS             <1..641
                     /locus_tag="IWO89_002592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002419316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS30-like element IS6770 family transposase"
                     /protein_id="HAQ0457291.1"
                     /translation="LYRLFSKGIFDIDTLPMKGKRKPNGHQEKRGKQQYQRSIHDRPD
                     NYPDFNSEFGHLEGDTIVGIHHKSAVITLVERLSKVIITIKPNGRKALDIETALNQWF
                     SRFPKNFFKSITFDCGKEFSNWKAISNQHDIDIYFADPGTPSQRPLNENSNGILRRNG
                     LPKSMDFRKVNQTFISSVSNQRNHIPRKSLNYRTPIEIFLSYVQEAFYSSLI"
     gene            complement(727..1821)
                     /locus_tag="IWO89_002593"
     CDS             complement(727..1821)
                     /locus_tag="IWO89_002593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF871 domain-containing protein"
                     /protein_id="HAQ0457292.1"
                     /translation="MGKLGISIYPERSTFEKDKAYLDLAHKYGYKRVFTSLLQINDDK
                     EKVLSEFKEVVDYANSLGMEVMVDINPALFEQLEISYDDLSFFHKMGAYGVRLDIGFT
                     GAEEAKMTRNPFGIKIEINMSSGTNYVDNIMSYSPNTDNLLGSHNFYPHRYSGLGYEH
                     FVFCSEKFRKYNLNTMAFVNSQSAEFGPWPTQDGLCTLEDHRDLEIATQVKHLILTGL
                     IDDISIGNAYASEEELKEMAEAFNADYPTLKVDTEEGITENERICLFDNLHSYRGDRS
                     EYILRSTMTRVYYKDKDFPPHNTRDMHHGDVLIDNEGYGQYKGETQIALKDMKNDGRV
                     NVVGRISDDELFLLDFLKPWSSFKLIENKK"
     gene            2010..2771
                     /locus_tag="IWO89_002594"
     CDS             2010..2771
                     /locus_tag="IWO89_002594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HAQ0457293.1"
                     /translation="MKKNKVILSISLAAALLFAAGCSANNTTSSSSSTESSKSTSTAE
                     STTASSVDLNALELPQLTKDVAENEDLVEMVTNKGTIKIKLFPEHAPKAVENFMTHAK
                     DGYYNGLTFHRVIKDFMIQGGDPKGDGTGGESIWGEGFETEINNHLYNIRGALSMARS
                     QDPNSNGSQFFIVQNTDDMHDGLLKDDYPQAIIDAYKNGGYPSLDGHYTVFGQVIEGM
                     DVVDAIANIETDSSDKPKEEVKIDKINILQEAKQK"
     gene            complement(2886..3326)
                     /gene="fabZ"
                     /locus_tag="IWO89_002595"
     CDS             complement(2886..3326)
                     /gene="fabZ"
                     /locus_tag="IWO89_002595"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HAQ0457294.1"
                     /translation="MSYVLSATEVMELIPNRYPICYIDYVDTLEPGKKIVATKNVTIN
                     EDFFQGHFPDNPVMPGALILETLAQAGSILILKSEEFLGKRAYIGGINKAKFRQKVVP
                     GDVLKCHFEITKMKGSVGTALSAAYVDDKKVCECEFTFIVNQNA"
     gene            3546..4301
                     /gene="fabI"
                     /locus_tag="IWO89_002596"
     CDS             3546..4301
                     /gene="fabI"
                     /locus_tag="IWO89_002596"
                     /EC_number="1.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-ACP reductase FabI"
                     /protein_id="HAQ0457295.1"
                     /translation="MSFLEGKKIVIMGVANKKSIAWGCAEAMLKQGAEIIYTYQNERM
                     KKSLVKLVGEEAFTVECDVSSDESIEKAMNAIGEYAGEIHGLVHAVAYANKEELSGNV
                     SDISRDGYQLAQDISSYSLIAVSKYAQPFLAPNSGIVSMTYLGSERAIPNYNMMGIAK
                     ASLEAAIRYLAAEFSPKGIRVNGISAGAIKTLAVTGVKDYQKLIQLSESRTPDQQGVT
                     IEEVGNACAFLISPLSSGIIGDIIYVDKGVHLS"
     gene            complement(4411..5424)
                     /locus_tag="IWO89_002597"
     CDS             complement(4411..5424)
                     /locus_tag="IWO89_002597"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid aminotransferase"
                     /protein_id="HAQ0457296.1"
                     /translation="MQIDWNNLGFSYIKTPWRFIAKWKDGQWEEGELTEDNYLTIHEG
                     SPALHYGQQCFEGLKAYRRKDGKVNLFRPEQNSRRMNQSARRLLMPMVPEKMFVDAVK
                     QVVKANEDFVPPYGTGATLYLRPLLIGVGENIGVHPAPEYLFVVFCTPVGAYFKGGLK
                     PTNFIVSDYDRAAPKGTGAAKVGGNYAASLLPGTEAKELDYSDCVYLDPATHTKIEEV
                     GAANFFGITKDGKFITPQSPSILPSITKYSLLQLAEERLGLQAIEGDIFIDDLDQFAE
                     AGACGTAAVISPIGGIYHNNNLHVFYSETEVGPVTKRLYDELTGIQFGDIEAPEGWIE
                     VVE"
     gene            5589..6509
                     /locus_tag="IWO89_002598"
     CDS             5589..6509
                     /locus_tag="IWO89_002598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel family protein"
                     /protein_id="HAQ0457297.1"
                     /translation="MFSLLATAGTTDSSGETLTDQAVSKVNAFQRFWDGIDWDAILAA
                     FIQKSLYLIFLIVLFWILHRVGKYLIDKSFNQYSKKAILNESRLKTLHSLLNTIFHYT
                     LGFFFIYAILSAIGVPIGSLLAGAGIAGVAIGLGAQGFMSDVITGFFIIMEQQMDVGD
                     YIKLANLTIEGTVVSVGIRTLQLKATDGTVHFIPNRNITTISNLSRANMQVLLDIRIV
                     PEEGYDKIYEIIDRVNRTLAEKYQEELQTEPSIFGLVDLGNSNFAIRTVCYALNGKQY
                     ALKEEFLSSYVKELTASGFTIPNSPIAVGK"
BASE COUNT         2029 a         1302 c         1169 g         2066 t
ORIGIN      
        1 cactttatcg tttattttct aaaggaatat ttgatattga cacactaccg atgaaaggta
       61 aaagaaaacc caatggccat caggaaaaac ggggaaaaca acagtatcag cgctcaatcc
      121 atgatcgacc tgataattat cctgatttca attctgagtt tggtcacctt gaaggtgata
      181 cgatcgttgg cattcatcat aaaagtgccg tcattacttt agttgaaaga ttatctaaag
      241 tcattatcac gattaaaccc aacggccgta aggcattaga tattgaaact gcccttaatc
      301 aatggttttc tcgcttccct aaaaacttct ttaaatctat tacgtttgac tgtggaaaag
      361 aattttctaa ctggaaagcc attagtaacc aacatgatat tgatatatat tttgcggacc
      421 ctggaacacc ttctcaacgc ccattaaacg agaattctaa cgggattctg cgtcgtaatg
      481 gactgccgaa atcaatggat tttagaaaag tgaatcagac atttatttcc agtgtcagca
      541 atcaacgtaa tcatattcca agaaaatcat tgaattacag aacaccaatc gaaatatttt
      601 tgagctatgt acaagaagca ttttattcta gcttaatttg acaaatcata acctataaaa
      661 aaacaaaata aaaaagcaga aataactggc tatttctagc ggattatttc tgcaataact
      721 aattatctat tttttatttt caattaattt aaagcttgac catggtttca aaaagtcaag
      781 gaggaataat tcgtcatcgg agattcttcc tacaacattg acacgtccgt cgtttttcat
      841 atccttcaag gcgatttgtg tttctccttt gtattgaccg tacccttcat tgtcaatcag
      901 tacatcccca tgatgcatat ctcgcgtatt atgaggcggg aaatctttat ctttgtagta
      961 tacacgagtc atcgttgaac gaaggatata ttctgagcga tcccctcgat aactatgaag
     1021 gttatcaaac aagcagattc gttcgttttc tgtaatacct tcttccgtat ctactttcaa
     1081 tgttggataa tccgcgttaa atgcttctgc catttctttc aattcttctt cacttgcgta
     1141 cgcatttcca atagaaatat catcaatcaa tcctgtaagg attaaatgct tcacttgtgt
     1201 cgcaatttca agatctcggt ggtcttccag cgtgcacaag ccatcttgcg taggccaagg
     1261 gccaaactca gcagattgcg aattgacgaa tgccatcgta ttcaaattat attttctgaa
     1321 tttttctgaa caaaaaacaa agtgttcgta tcctaaacca gagtatcgat gaggataaaa
     1381 attatgagaa cctaataaat tatccgtgtt tggcgaataa ctcatgatgt tgtcaacgta
     1441 attcgtgccc gaactcatat tgatctcgat tttgatacca aaaggattgc gcgtcatttt
     1501 tgcttcttct gctcctgtga atccgatatc taagcggacc ccgtaggcac ccatcttatg
     1561 gaaaaaagat aagtcatcgt aagaaatctc caactgttca aataacgctg gattgatatc
     1621 aaccattact tccatgccta ggctattcgc gtaatcaact acttctttaa attctgaaag
     1681 aactttttct ttatcatcat tgatctgcag caaactggta aaaacccgtt tatacccata
     1741 cttatgagct aaatccaagt acgctttgtc cttttcaaat gttgaccgtt ctggataaat
     1801 cgagattcct aattttccca tattaccatt tcctcctgca tcttttcgtt tttgtatcca
     1861 cttacattat agcaaacaca aagtaaatgg tctacacctt ttctttttct tttaaaataa
     1921 atgataaatt ccaataattt aagtaggtat tttccctcta ttttgttatc attaaagata
     1981 acaaaatagt aaattggaaa ggaacagtta tgaagaaaaa taaagttatt ttaagtatta
     2041 gtttagcagc agcattgctg tttgctgctg gttgttctgc aaacaacacg acttcttcta
     2101 gttcgtctac agaaagttca aaaagtacat ctactgcgga aagcacgact gccagtagcg
     2161 tcgatttgaa cgcattggaa ttaccacaat tgacgaaaga tgtcgccgaa aatgaagatc
     2221 tggtcgaaat ggtgacgaat aaaggcacaa tcaaaatcaa attatttccc gaacacgcac
     2281 caaaagcggt tgaaaacttt atgacacatg caaaagacgg ttactacaac ggactgactt
     2341 tccatcgcgt catcaaagat ttcatgatcc aaggtggcga tcctaaaggc gatggcactg
     2401 gaggggaaag catttgggga gaaggatttg aaacagagat caacaaccac ctttataata
     2461 taagaggtgc gttatctatg gctcgctcac aagatcctaa cagtaacggc agccagttct
     2521 ttatcgttca aaatacagat gatatgcatg atggtctttt aaaagacgac tatcctcaag
     2581 caatcatcga cgcttataaa aacggcggtt atccttcact agatggccat tatactgtct
     2641 tcggccaagt gattgaagga atggatgtcg tggatgctat tgcaaatatc gaaacagata
     2701 gttcagacaa accaaaagaa gaagtaaaaa tcgataaaat caatatactg caagaagcaa
     2761 aacaaaaata aaaaaataac gtctgaatcc tgcatagatg acaacgtatg ctaggattca
     2821 ggcgtcttct ttttacataa aaagcaagta agcttccttt tgttttgcta tatcaggttt
     2881 tatagtcaag cattttggtt gacgatgaac gtgaactcgc actcacagac ttttttatca
     2941 tctacatatg ctgcagataa cgctgtaccg actgatccct tcattttggt gatttcaaaa
     3001 tgacatttca gcacatctcc tggaacaact ttttgacgga atttggcctt attgatccca
     3061 ccgatataag ctctttttcc tagaaactct tctgatttta ggataagtat cgaacctgct
     3121 tgtgccagtg tttctagaat caacgcaccc ggcatcactg gattgtcagg aaagtgacct
     3181 tggaaaaaat cttcattgat tgttacattt tttgtcgcaa cgatcttttt tcctggttct
     3241 aaagtatcta cataatcaat ataacaaata ggataacgat taggaatcaa ttccattact
     3301 tcagtagcag ataatacgta agacataatt aaactccttc tttcaggcaa ttgccatttt
     3361 cattactttg actatcaaag tatcatattc ctaaatattt agcaatcttt ttttaattta
     3421 agaaaaaata gtttgacaat caaaacattt tgactaataa ttaagaaatc aagaagttat
     3481 tttagctatt attctctatt tttttacgac tatcaaattt ttatcagaaa caaaggagag
     3541 tcattatgtc atttctagaa ggaaaaaaaa tcgtcattat gggcgtcgct aacaaaaaaa
     3601 gcattgcttg gggttgtgct gaagcaatgt tgaaacaagg ggcagaaatt atctatactt
     3661 accaaaatga acgaatgaaa aaatcattag tcaaattggt cggcgaagaa gcattcactg
     3721 tagaatgtga tgtttctagc gacgaaagca tcgaaaaagc aatgaatgcc atcggtgaat
     3781 atgctgggga gatccacggc cttgtccatg cagttgctta cgccaacaaa gaagaattgt
     3841 ccggaaatgt gtctgatatt tccagagacg gctatcaatt agctcaagat atcagcagct
     3901 actctttaat tgctgtatca aaatacgcac aaccgtttct tgcaccaaac agcgggatcg
     3961 ttagcatgac ttatctaggt tcagaacgcg caatcccgaa ttataacatg atgggtattg
     4021 cgaaagcttc tcttgaagct gctatccgtt atttggcagc tgagttctcg cctaaaggaa
     4081 ttcgggtcaa tggaatctca gctggggcaa tcaaaacatt agctgtcaca ggtgttaaag
     4141 actatcaaaa actgatccag ttatctgaat ctcgtacgcc tgatcagcaa ggtgtgacga
     4201 tcgaagaagt cggtaatgct tgtgctttct taatcagccc actttccagc ggaatcatcg
     4261 gtgatattat ctacgttgac aaaggtgtcc atctttcata aacttttgtt gcattcttta
     4321 aagtaaaaca tataaaaatc aggaaaaaac tttttaaagt ctcttcctga ttttttcttt
     4381 ttacgttttt ttcaactcat tactttgaaa ttattccacg acttcaatcc agccttctgg
     4441 tgcttctatg tctccaaatt gaatacctgt caattcatca tacaaccgtt ttgttactgg
     4501 tcctacttcc gtttcactat aaaatacatg aagattgtta ttgtgataaa tcccaccgat
     4561 tggagaaata acagccgctg taccacatgc accagcttct gcaaattgat ctagatcatc
     4621 aatgaaaata tctccttcaa ttgcttgcag tcctagtcgt tcttcagcta actgcaacaa
     4681 ggaatatttc gtgatacttg gtaagataga gggcgattgc ggagtgatga attttccgtc
     4741 ttttgtaatt ccaaagaaat ttgctgcacc tacttcttcg attttcgtat gtgtggctgg
     4801 atcaagataa acacaatctg agtaatccag ttcttttgct tctgttcctg gaagtaaact
     4861 cgctgcgtaa tttccgccga cttttgctgc accagtcccc ttaggagcag cacgatcgta
     4921 atcagataca ataaaattcg ttggttttag tccacctttg aaatacgctc ctactggcgt
     4981 acagaagacg acaaataaat attctggagc tggatggaca ccaatgtttt caccgacccc
     5041 aatcagtaaa gggcgcaagt ataatgtagc gcctgttcca tagggcggca caaaatcttc
     5101 atttgctttt accacttgct tgacagcatc aacgaacatc ttttcaggga ccattggcat
     5161 cagcaatcgt ctagcacttt gattcatccg tcgactattt tgttcaggtc gaaagagatt
     5221 gaccttgccg tcttttcgac gataagcttt tagaccttca aaacattgct gaccataatg
     5281 aagcgctgga gagccttcat gaatcgtcag gtaattatct tctgtcaatt ctccttcttc
     5341 ccattgtccg tctttccatt tggcaataaa acgccaagga gttttgatat aagaaaaacc
     5401 tagattgttc caatcgattt gcatatactt accaccttcg ttattttctc cacattttat
     5461 cattatccat attatttgtc attttatttt cagctttttt ccttcattat agttattttt
     5521 gctatttcct tttttgaaaa cttcttggta caatacagaa agaatcaaat tttagaaaag
     5581 aggattttat gttttcatta ttagcaactg ccggtacgac agactcttct ggcgagacat
     5641 tgacagatca agctgtcagc aaagtaaatg cgtttcagcg gttttgggac gggattgatt
     5701 gggacgccat tttggctgcc tttatccaaa aaagtttata tctgattttt ttgatcgtcc
     5761 ttttttggat tcttcaccgt gtgggaaaat atctgattga taagtctttt aatcagtatt
     5821 ctaaaaaagc cattctaaac gagagccgct taaagacttt acatagtctt ttaaatacta
     5881 tttttcatta tacccttggt ttctttttta tctatgcaat tttatctgct atcggtgtgc
     5941 cgatcggatc acttttggct ggtgccggaa ttgcaggtgt ggcaatcggt cttggtgccc
     6001 aagggttcat gagtgatgtt attaccggat tttttattat catggaacaa cagatggatg
     6061 ttggggacta catcaagcta gcaaacttaa cgattgaagg aaccgtcgta tctgttggta
     6121 tccgtaccct tcagctgaaa gcaacggatg gtactgtcca tttcatcccg aatcgaaaca
     6181 tcaccacgat cagcaatctc tctcgtgcaa atatgcaagt acttttagat attcggattg
     6241 ttcctgaaga aggatatgac aaaatctatg agatcattga tcgagtaaat cgtacattag
     6301 ctgaaaaata ccaagaagag ctccaaactg aaccttctat ctttggatta gtggatcttg
     6361 gaaacagcaa ttttgcgatt cgtacggtct gctacgcatt gaacggaaaa caatatgcct
     6421 taaaagaaga gttcttgagc tcctatgtca aagaattgac agcttctggt ttcacgattc
     6481 cgaacagtcc aattgctgtc ggaaaataat ttggacaaac gatgcaacga atctcactaa
     6541 aaagagcgtg ggacaaaagt aaagaa
//
LOCUS       DACEEZ010000115         6542 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20313_2017
            SAMN12639966-rid9879223.denovo.115, whole genome shotgun sequence.
ACCESSION   DACEEZ010000115 DACEEZ010000000
VERSION     DACEEZ010000115.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639966
            Sequence Read Archive: SRR10023158
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6542)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6542)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741683.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741683.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:44:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,016
            CDSs (total)                      :: 2,962
            Genes (coding)                    :: 2,889
            CDSs (with protein)               :: 2,889
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 36 of 73
            Pseudo Genes (incomplete)         :: 29 of 73
            Pseudo Genes (internal stop)      :: 18 of 73
            Pseudo Genes (multiple problems)  :: 8 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6542
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639966-rid9879223.denovo.115"
                     /isolate="E20313_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(7..1164)
                     /locus_tag="IWO89_002599"
     CDS             complement(7..1164)
                     /locus_tag="IWO89_002599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0457298.1"
                     /translation="MRTIQRKLRELNTILKNYHLSCSLKKGNPIEGEEYRIRYFFHLM
                     YWQSFDENHSDILSSIKNKRQLLEKFAKHAPYTRSIDRKKWISLLRISLQRMKQGHSI
                     TYVPEEMNKFVHPLITKEEFTHYILEPFFEANFWSMNHITPTEQAYLYFMFGVMNTYL
                     EEDLLDTPDKVQESWGWIESSIVENFEKNFKICFTTSEKNYLYVNLAMIHLYSRVFGT
                     KKKVDAFGKSAEDRDFQHIFPVMYPIMQQFFSGVARRIPELNTYYEKNRRLIFQYCML
                     VRDIFIKHEQPVIFYIQSKYGKTQELWAKKRILSMTERPIKYSASADQLPDIVISDYP
                     IDKKAYDQTNTQIFYWNGQPTKNDWARLKAEIIKIRNEKNRSILEKLDFVN"
     gene            complement(1167..1523)
                     /locus_tag="IWO89_002600"
     CDS             complement(1167..1523)
                     /locus_tag="IWO89_002600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0457299.1"
                     /translation="MFEEFIDINERQVYQFLNYCYERDEKLYVVKDIALDLNYTLAKM
                     NSVIQQAESFCERYPEYKLSFLSENKMIKVEFSSQFLLSKVYSILLEGTIGYILLDSL
                     YKGTYQSLENLSQKII"
     gene            complement(1821..2741)
                     /gene="sgrA"
                     /locus_tag="IWO89_002601"
     CDS             complement(1821..2741)
                     /gene="sgrA"
                     /locus_tag="IWO89_002601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG-anchored fibrinogen/nidogen-binding
                     adhesin SgrA"
                     /protein_id="HAQ0457300.1"
                     /translation="MKKTATVLFVILGLSIIAAMIGISTSANERANENLSFTVKTDRI
                     VYDMTQQVITIPVKPNKSVNASDVHAVLTYGWDGNGSSEKVIGEVYLKDVQWTAGIEY
                     TIMISAELSIDEIKSKDKVDLIVFYDGQMTITENLKPSSWTVVGPPSSTDSTSSESST
                     ENTSGESSTESTSSESSTENTSSESSTESTSSESSTGSMSSESSTGSTSSESSTGKVN
                     NELDLVPIIPTKEQKRNAGSNLKSVLKQPDFVSRGIDHQEKAKETDNRKSDLPETGSR
                     TLNKLITWMGVLLILIVGASYFRSLFHRVK"
     gene            complement(2937..3776)
                     /locus_tag="IWO89_002602"
     CDS             complement(2937..3776)
                     /locus_tag="IWO89_002602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002378019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HAQ0457301.1"
                     /translation="MIRIIRLIGSVLIVLVGFVLGILVNNALADMFIGNSEWIGAIAG
                     TVGHLAVFLLALFLASKVEGKRIEDYGVSVRGKDWGYLAGGLVVGIGVFLLITSPLYL
                     TGAYRLDSGNANIVPLITSFILFIAVGASEELLFRGFFQHQMLRFGPLIAMIGSAALF
                     ALLHGLNPNMTFLAVFNIFLAGCFFSALIYSTGSLFTAIGAHITWNWTQGAILGIPVS
                     GTQEQGFFSTLIQSQNTIITGGNFGAEASISTTLFLLVLIVGLLLYAYKTKRMKKYTA
                     VYQ"
     gene            3961..4848
                     /locus_tag="IWO89_002603"
     CDS             3961..4848
                     /locus_tag="IWO89_002603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rotamase"
                     /protein_id="HAQ0457302.1"
                     /translation="MGNFLKKQLLIMCLSACAILLFSGCSKSNETIATFKGGTLTVQD
                     FYDNPKVKSNNQTLLKKMIVFKAFDDVYGKEISSKEVTKEYNEQIKKLGPNYKEQLKA
                     VGQTEETYKKLFKQMLAFQYGLKANVKLTDKDLDTAWKEFYPEVSTQIILFSTKEEAD
                     KAKKEANEGENFSKLVQAYGKNKTLKETDGKMNFDSTNPEIPTEVKKAAFKLKNGEVS
                     DIIPVTDPTTYQQSYYLVKMVKKQDKGSNKDKYKSELEKIATEAKTLDTEFMDKTIRK
                     VMKKDNVTIKDPYVKNIFK"
     gene            complement(5432..6127)
                     /locus_tag="IWO89_002604"
     CDS             complement(5432..6127)
                     /locus_tag="IWO89_002604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-6-phosphate aldolase"
                     /protein_id="HAQ0457303.1"
                     /translation="MEIMLDTANTKSIYSCQQFLNLTGVTTNPTILKKEGASNFFSHM
                     KEIKKIIGQIPIHIQTIGRTEEEMIEDARVIIEELGKDTFIKVPVNQAGLQAIKKLKK
                     EGYLITGTAIYTELQGYLAINNGADYIAPYYNRMQNQGIDAMKVIDSLRTEIERTQRP
                     TKILAASFKNCEQVTTAIRSGAHAITLGKELLDQVLEMPSIDKAVRGFQEDWVQTFQA
                     ETIGELKKVADYF"
     gene            complement(6111..6509)
                     /locus_tag="IWO89_002605"
     CDS             complement(6111..6509)
                     /locus_tag="IWO89_002605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIA"
                     /protein_id="HAQ0457304.1"
                     /translation="MTIFETVVTDVGIEAEVFRKEKIMILFGDEAPETLTDYCYKIEL
                     NQVRKEITCDMKLCFDKQMYVITAVGDVVQTNLNELGHITIKFDGSHEPELPGTMYVE
                     DKEMPHLRKGTIIKIDQEDQNGGKLYGNHA"
BASE COUNT         2067 a         1289 c          960 g         2226 t
ORIGIN      
        1 cttttcttaa ttaacgaaat ccaatttttc caatatggat ctatttttct cattcctgat
       61 ttttatgatt tcagctttca gacgcgccca atcatttttt gtaggttgtc cattccagta
      121 aaatatctgt gtattcgttt gatcatatgc ctttttgtcg ataggataat cgctaatgac
      181 aatatcaggt aactgatcag cggatgcact atattttatt gggcgttcag tcatagataa
      241 tatccttttc tttgcccata attcctgtgt tttaccatac tttgattgaa tatagaatat
      301 gacaggttgt tcgtgtttta taaaaatatc cctgactaac atacagtatt gaaaaatcaa
      361 ccgtctgttc ttttcataat aagtattaag ctctggaatt ctgcgagcta ccccactaaa
      421 aaactgttgc atgattggat acatcactgg aaatatatgc tgaaaatctc tgtcttctgc
      481 tgattttcca aaagcatcca ccttcttttt tgtcccaaaa acccttgaat acaaatggat
      541 cattgccaaa ttgacatata gataattttt ttctgaagtt gtaaaacaaa tcttgaagtt
      601 tttttcaaaa ttttctacaa tagacgattc aatccagccc caactttctt gtactttatc
      661 tggagtatcc aacaaatctt cttccaaata ggtgttcatt acaccaaaca taaaataaag
      721 gtaagcttgc tctgttggag tgatgtgatt catcgaccag aagtttgctt caaaaaaagg
      781 ctctagtatg taatgcgtaa actcttcttt tgttatcaat ggatgcacaa atttgttcat
      841 ctcttctggg acatacgtaa tcgagtggcc ttgtttcatt cgttgcaacg aaattcttag
      901 taggctgatc catttctttc tatctatact acgcgtatat ggcgcatgtt tcgcaaattt
      961 ttctaatagt tgacgtttat tttttatact tgataatata tcagaatgat tttcgtcgaa
     1021 tgactgccaa tacatcaagt gaaaaaagta tcggatacgg tattcttctc cttctatcgg
     1081 attacctttt ttcaagctgc aagataaatg atagtttttc aaaatagtat taagttctct
     1141 taattttctt tgaatcgtac gcaaacttat ataatttttt gactaagatt ttctaaggat
     1201 tgataagtac ctttgtaaag tgaatccagt aaaatataac caatcgttcc ctctaaaagt
     1261 atagaataaa ctttactcag taaaaactga gaagaaaact cgacttttat catcttgttt
     1321 tctgaaagaa aagataattt atattctgga tacctttcgc aaaacgattc tgcttgttga
     1381 atcaccgaat tcatcttagc cagggtgtag tttagatcta aagcgatatc cttcaccaca
     1441 tataatttct cgtctctttc ataacaatag ttcaaaaatt gatatacttg cctttcattg
     1501 atatcaataa attcttcaaa caaagtacat actccttttt atctattatt tagacttatt
     1561 ccgacagcta catgaataac gtacaaatag attcgttact gatttttcag agatactacc
     1621 atgcgcagtg tgcattgtca attattcttg ctcttatttt ccatttgtgt tcctgttatt
     1681 tcctgtttct caaatatgtt agtaaacaaa aatagatctg gcaaagctac aacttcgcca
     1741 ggcaatacag cactctcgaa tgaaatattc actcaaaaat ctatcgtttt attcaatttt
     1801 agatctgttg cttttattct ttatttcact cgatggaaga gagaacgaaa atagcttgct
     1861 cctacaatca aaatgagtaa cacacccatc catgtaatta gcttgttcaa ggttctacta
     1921 ccagtttctg gtaaatcgct ttttctatta tctgtttctt tcgctttttc ttgatgatca
     1981 attccacgag aaacaaaatc aggctgtttt agcacacttt ttaggttgct ccctgcattt
     2041 ctcttttgtt ccttagttgg tatgataggt accaaatcaa gttcattatt tacttttcct
     2101 gtgctgcttt cgctgctcgt gcttcctgtg ctactctcac tactcatgct tcctgtgcta
     2161 ctttcactac tcgtactctc cgtactgctt tcgctgctcg tgttttctgt gctactttca
     2221 ctgctcgtac tctctgtact gctttcaccg ctcgtgtttt ctgtactgct ttcactactt
     2281 gtgctatccg tactgcttgg cggtccaaca actgtccaac tgcttggttt caaattctcc
     2341 gtgatcgtca tctgtccatc ataaaaaaca atcaaatcaa ctttatcttt actcttgatc
     2401 tcatcaatcg atagttctgc agaaatcatt attgtatact caataccagc ggtccattgt
     2461 acgtctttta aataaacttc cccaataact ttttctgaac ttccattacc atcccaacca
     2521 tatgttagaa cagcatgtac atcactagca ttcacgcttt tgtttggttt gactggaata
     2581 gtaatgactt gttgtgtcat gtcataaaca atccgatcag ttttcacagt aaatgaaaga
     2641 ttctcatttg cccgttcatt tgcagaagta ctgattccaa tcattgctgc aattatactc
     2701 agcccaagaa taacaaataa tacggtcgct gttttcttca tttctatcac tctccatata
     2761 attaccttct atttgataaa ggaattatat aaaatgaaaa tgatgagaac acgacagtca
     2821 gttgtgaaaa tctaacaaaa gatgtcgtgt attcgcttga atttgtttac tttttcgttt
     2881 aatcaatgac agacttaaaa aagcaagacc gatatcagcc ttgctttctt catcttttat
     2941 tgataaacag cagtgtattt cttcatccgc tttgttttat atgcatagag taacaaacca
     3001 acaatcaaga ctaacaagaa taatgtagtt gatatagatg cttcagcccc aaagtttccc
     3061 ccagtaatta ttgtattttg cgattggatc agagtggaga aaaaaccttg ttcttgcgtt
     3121 ccgctgactg gtatacccaa aatagctcct tgtgtccaat tccaagtaat atgagcacca
     3181 atagccgtaa ataaacttcc tgtactgtag ataagtgcgc tgaaaaagca acctgctaaa
     3241 aagatattga aaactgccag aaatgtcatg ttaggattca atccgtgcaa aagagcaaat
     3301 aatgcagcag atccaatcat tgctattaac ggaccaaatc taagcatctg atgctgaaaa
     3361 aagccacgga ataaaagctc ttctgatgcc cccactgcaa taaataaaat aaaactcgtt
     3421 atcagcggca caatgtttgc gtttcctgaa tctaaccgat acgcccccgt caaatataat
     3481 ggactagtaa taagcagaaa cactccaatt ccaacaacga gacctcccgc taaatacccc
     3541 cagtcttttc ctcgaacaga aacaccataa tcttctattc tttttccttc gaccttacta
     3601 gcaagaaata aagctagtaa aaaaacagct aaatgtccga ccgtaccggc aattgctcca
     3661 atccattcac tatttccaat aaacatatcc gcaagtgcat tattgacaag aattcctaaa
     3721 acaaaaccaa ctaagactat taaaacagat ccaatcaacc gaataatcct gatcatcttt
     3781 catccctcct ttatattaag tttaacaacc cttttctttc ttgtctcgtg aaaactatgt
     3841 aaatgttctc catcatagag agaatagatt tttacgaact aagaatatct acagcaacct
     3901 tacgaaaatg aaagactgtc tccctaattc ttctgttaga ctgtttttaa taaggaaagg
     3961 atgggaaatt ttttgaaaaa acaactacta attatgtgcc tttcagcatg tgcaatttta
     4021 ctattctcag gttgtagcaa gtccaatgaa acgattgcaa cgtttaaagg tggaacactt
     4081 acagttcagg atttttatga taacccaaaa gttaaatcaa ataatcaaac actattaaaa
     4141 aaaatgattg tcttcaaagc atttgatgat gtatacggaa aagagatttc ttcaaaggaa
     4201 gtaacaaaag aatataatga acaaatcaaa aaactcggtc caaactacaa ggaacaatta
     4261 aaagcagttg gccaaacaga agagacatat aagaaactct ttaaacagat gctggctttc
     4321 caatacggtt tgaaagccaa tgtaaaatta acagataaag atctagacac tgcttggaaa
     4381 gaattctatc cagaagttag tacacagatc attctatttt cgactaaaga agaagcagat
     4441 aaagcaaaaa aagaagcaaa tgaaggggaa aatttctcca agcttgttca agcttatgga
     4501 aaaaataaaa cattaaagga aactgatgga aaaatgaatt ttgattcaac caatccagag
     4561 attcctactg aagtaaaaaa agcagcattt aaactaaaaa acggggaagt ttctgatatc
     4621 attcctgtaa cagatccaac gacttatcag caaagctatt atctagtaaa aatggtaaaa
     4681 aaacaggata aaggttctaa taaagacaaa tacaaatctg agctagaaaa aattgctacc
     4741 gaagcaaaaa ctcttgatac ggaattcatg gataaaacaa tccgtaaagt gatgaaaaaa
     4801 gacaatgtaa ctattaaaga tccttatgta aaaaatatat tcaaataaac aaatttgacc
     4861 caattgggga tgtggcaaaa tttgagaagt gatttttgga aattccttct tatttttgag
     4921 gcaaaccact tttgccacaa cttcatcggg gccttttttt atttgtacca aaacaaatca
     4981 ctggatttgt tacaatcatg aggatataat ttaaaaaagt tttttatttc tatcacatga
     5041 atcaatgcgc cattaagtag attctttcat gcttccctat tttttcactt gccgtatcgc
     5101 ttcaaaaaca actatcataa actcaaactc gactgaaaag ttattttaaa aaagactaaa
     5161 aatatccctt ctggacttgt tcctcttatt ctgttatact agagaaacac gaaagatatt
     5221 tttatgcgac acttttcatt tgtttttatg aaatttattc aaaactggcg taacttcaga
     5281 gttacaagga tgtattagag gttagggaat acatcgttta aatgagaaat aagtaatagt
     5341 cacccaaact ttaaattaat gaatattgaa tcgtcagata gcctcctcga gttttcgaga
     5401 aggctatcgt ttattttatg gagaaattga ttcagaagta atcagcgacc ttttttagtt
     5461 caccaatcgt ttctgcttgg aaagtttgca cccaatcttc ttggaagccc ctcactgctt
     5521 tatcaatcga gggcatctct aatacttgat ctagtagctc tttacctaac gttatcgcat
     5581 gagctccaga tcgaatggct gtagtgactt gttcacaatt tttgaaacta gctgctaata
     5641 tttttgttgg tctttgcgta cgttcgatct cagtccgcaa cgaatcaatc actttcatag
     5701 catcaatgcc ttgattttgc atcctgttat aataaggtgc aatataatct gctccattat
     5761 taatagctaa atacccttgt aattctgtgt aaatagctgt accggtaatc aaataacctt
     5821 cttttttcaa ctttttgatc gcttgaaggc ctgcttgatt caccggcacc ttgataaacg
     5881 tatcttttcc cagttcttct atgataactc gagcatcctc tatcatttct tcttccgttc
     5941 ttcctattgt ctgaatatgg ataggaatct gtccgattat ttttttgatt tctttcatat
     6001 gcgagaagaa gtttgacgct ccttcttttt tcagaattgt aggatttgtt gttacgcctg
     6061 ttaaattcaa aaactgttga caagaataaa tactttttgt atttgctgta tcaagcatga
     6121 tttccatata gctttccccc attctgatcc tcttgatcga ttttaatgat tgtccccttt
     6181 cttaaatgcg gcatttcctt atcttctaca tacattgttc ctggaagttc cggctcatga
     6241 gatccatcaa attttatcgt gatatgtcct aattcattca aattagtttg gacaacatct
     6301 cctactgctg tgattacata catttgttta tcaaaacaga gcttcatatc acaagttatt
     6361 tcttttctta cctgattcaa ttcaatctta taacaataat ctgttaatgt ttccggtgct
     6421 tcatctccaa ataaaatcat tattttttct ttcctgaata cttctgcttc tataccaaca
     6481 tctgttacaa ccgtttcaaa gatcgtcatc tctgttcctc ctcgtaaaat atcatttgtc
     6541 ta
//
LOCUS       DACEEZ010000116         6496 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20313_2017
            SAMN12639966-rid9879223.denovo.118, whole genome shotgun sequence.
ACCESSION   DACEEZ010000116 DACEEZ010000000
VERSION     DACEEZ010000116.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639966
            Sequence Read Archive: SRR10023158
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6496)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6496)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741683.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741683.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:44:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,016
            CDSs (total)                      :: 2,962
            Genes (coding)                    :: 2,889
            CDSs (with protein)               :: 2,889
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 36 of 73
            Pseudo Genes (incomplete)         :: 29 of 73
            Pseudo Genes (internal stop)      :: 18 of 73
            Pseudo Genes (multiple problems)  :: 8 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6496
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639966-rid9879223.denovo.118"
                     /isolate="E20313_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(<1..2532)
                     /locus_tag="IWO89_002606"
     CDS             complement(<1..2532)
                     /locus_tag="IWO89_002606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction endonuclease subunit R"
                     /protein_id="HAQ0457305.1"
                     /translation="MKISHREEAEVEEQLIRVLGEGHNQWTYRPDLKSEEDLWSNLRQ
                     KIISNNQAELNDSPLTDKEFETIKTELLLRTKTPFDAAKWLKGENGMARITIERENPQ
                     LGSVSLILYSNQDIGGGISTYEVVHQIAKRGSNIEARDRRFDVTLLINGLPIIQIELK
                     QVTAKDGFYQAFNQIKKYAEEGMFRNNIFSTLQLFVVSNEQTTRYFANALPKDLHPKF
                     LFSWRTKDNKKVENLYEFCKQVLNIPDAHRLIADYTIVSEDQDNKTLMVLHPYQVHAI
                     QALFIAANKHQSGYVWHATGSGKTLTSFVSTKLLARKSGIDRTIMLVDRKDLDNQTTT
                     EFTKFASEFNTGVSSGNAKANSLIVGTGSAKELSETLLADANANVVIITTRQKLDAAL
                     KYAKKQEEKKGTNRFQKLMGQHIVFVVDECHRALSAENMEEIKKMFPKSTWFGFTGTP
                     IFPENRKQAKGQLARTTHDQYGEVLHTYTIKNALEDGSVLGFQVEHENTVEPTSLENK
                     IYRKLKEVETYAEYSPEQINRMIDQMESVKKESYLDPTVFEAEEHIQKVIHKMFRPDN
                     AYTKFDFRNGRPTKSAILTTSSIDMAKKYYRAIKEMTKESDWLIKEFPNQPIREGRTM
                     EDPDFPRIAITYSLEENSRDSSAQQEEMQKIIEEYNQYYDTAWSLADIERYNGDINNR
                     LARKRAEFKQFGKQIDLVIVVDRLLTGFDAPTIQTLFVDRNLEYAGLIQAFSRTNRTY
                     PEKTKGLIVTFRKPATMEKNVEDATKLYSEAKEESGLIYPSYQESKKRFKQAYGKLKE
                     VSSMESIDEHTPLEIRIEYVKAFQELNRAYESLVTYDDYNDDMVQS"
     gene            complement(2546..3763)
                     /locus_tag="IWO89_002607"
     CDS             complement(2546..3763)
                     /locus_tag="IWO89_002607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HAQ0457306.1"
                     /translation="MSNDTRPEIRFPGFTEDWEERKLGDMMDVTSVKRIHQSDWTNSG
                     IRFLRARDIVSAAKNEEPSDYLYISEEKYNEYSKNSGKVSQGDLLVTGVGSIGVPLLI
                     TDDNPIYFKDGNIIWFKNEHKIDGNFFYYSFINNKIQKYIRDVAGIGTVGTYTIDSGK
                     KTPISLPTYDEQIKIGSFFKQLDHTIMLHQHELDLLKETKKGFLQKMFPKNGAKVPEV
                     RFPGFTGDWEERKLGEVVVFLNGRAFKQQELLDSGKYRVLRVGNFNTNDRWYYSNLEL
                     EEDKYANKGDLLYLWATSFGPEMWNEERVIYHYHIWKLKFLSKELDKQYLYTWLLTDK
                     EKIKESTNGTTMVHVTKGGIEQRTFQFPPDINEQQKIGAFFKQLDDTIALHQRKLDLL
                     KETKKGFLQKMFV"
     gene            complement(3753..5348)
                     /locus_tag="IWO89_002608"
     CDS             complement(3753..5348)
                     /locus_tag="IWO89_002608"
                     /EC_number="2.1.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction-modification system subunit
                     M"
                     /protein_id="HAQ0457307.1"
                     /translation="MGAELNQKLFSAADNLRSKMDASEYKNYLLGLIFYKYLSDRLLE
                     QVVLLADESLEEYDTVSKQTMLYRELLSDEESKEDLIATIVDILGYSISPEYLFNVLA
                     DQAKQATFQLNDLNKAFVQLASTYNQFNGLFDDVDLQSKKLGTDEQQRNVTITEVIKK
                     LNDVEVLGHDGDVIGDAYEFLISQFASEAGKKAGEFYTPHMVSDMMAQIVTLDQKERR
                     FFSVFDPTMGSGSLMLNVRNYLTHPDNVKYHGQELNTTTYNLAKMNLILHGVDAEEMN
                     LRNGDTLNKDWPTDEPYTFDAVVMNPPYSANWSADTTFLDDSRFNRYGKLAPKSKADF
                     AFLLHGFYHLKETGTMAIVLPHGVLFRGAAEGVIRQKLLEDGSIYAVIGMPANLFFGT
                     SIPTTVIVLKKNRQTRDVLFIDASREFVKGKNQNKLSEENIQKILETYAERKDVEKYA
                     HLATFDEIKENDYNLNIPRYVDTFEEEEPIDMVHVGNDIKKIRQEQQVLEKELLEAIS
                     SLQTTPENEAWLQGALEVFKHEQ"
     gene            5473..6258
                     /locus_tag="IWO89_002609"
     CDS             5473..6258
                     /locus_tag="IWO89_002609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SinI family restriction endonuclease"
                     /protein_id="HAQ0457308.1"
                     /translation="MPNKNRTFESILNEGLTTEPASIIFDEVIDDVEDSKYHKNLKEL
                     FYFALNNGPSLFTGISVPDSDNPLIMAKIYLTKWCNKYIRDRNNPALKKPLKTFGEKD
                     AALTTRVAANANIDDQKVLNDYLRGHFLYMSAENMNGSILEEYLAEVLEPEGWIWCAG
                     SVYRAVDFCYLGTSPILLQVKNKYNTESSSSSAIRVGTTIRKWNRLNKSTKISGLDSP
                     IPNWKALIEMTEASKELAAKLTENSYLAYINEKSTRELWTLDD"
BASE COUNT         1897 a         1319 c         1094 g         2186 t
ORIGIN      
        1 agattgcacc atgtcatcat tgtaatcatc ataagtcact aacgattcat acgcacgatt
       61 caattcttga aatgctttga cgtactcgat acggatttca agtggcgtat gttcatcaat
      121 ggattccatg gatgaaacct cttttaactt accatatgct tgcttgaatc tttttttgga
      181 ttcttggtaa cttggataaa tcaaacccga ttcttcttta gcttctgaat acagttttgt
      241 cgcatcctcg acattcttct ccattgtcgc tggctttcgg aaagtaacaa ttagtccttt
      301 tgttttttca ggatacgtac ggttcgtacg tgaaaaggct tggatcaatc ctgcatactc
      361 tagattacga tccacaaata gggtttgaat cgtaggggcg tcaaaacctg tcaatagacg
      421 atccactacg attactaaat cgatttgttt cccaaattgt ttgaattccg ctctttttcg
      481 tgccaaacga ttattgatgt ctccgttata acgctcaatg tctgctagtg accaggcagt
      541 atcataatac tgattatatt cctcaataat tttttgcatt tcttcttgtt gggcacttga
      601 atctcggcta ttttcttcta atgaataggt aatagcgatt cgtgggaaat caggatcctc
      661 catcgttcgt ccttcacgga taggctgatt gggaaactct tttattaacc aatcagattc
      721 tttcgtcatc tctttgatgg cccgataata ctttttggcc atatcaatgg aactcgtggt
      781 taagatcgca gacttcgttg gacggccatt tcgaaaatca aattttgtat aggcattgtc
      841 tggacggaac attttatgaa tgactttttg gatatgttcc tctgcttcaa aaacagtagg
      901 gtccaaataa ctttcttttt tgacagactc catttgatcg atcatacgat tgatctgttc
      961 cggtgaatac tctgcatatg tttctacttc ttttaatttc cgatagattt tattctctaa
     1021 tgaagtcggc tccacggtgt tttcatgctc tacttgaaat cctaaaaccg agccatcttc
     1081 taatgcattc ttgatggtat aggtgtgcaa gacctctcca tactgatcat gggtcgtacg
     1141 tgccaattgc cctttggctt gtttccgatt ctcagggaaa atcggtgtac ctgtaaagcc
     1201 aaaccaagtg gatttcggaa acattttttt gatttcttcc atattttcag cacttaacgc
     1261 tcgatgacac tcatcaacta caaacacgat atgttgcccc attaattttt ggaagcgatt
     1321 ggttcctttt ttctcttcct gtttctttgc atatttcaac gccgcatcta gtttttgacg
     1381 ggttgtaatg atcacaacat tcgcattggc atctgccaac agagtttcac ttaactcttt
     1441 ggcacttcct gttcccacga tcaagctatt cgctttggca ttaccagaag aaaccccagt
     1501 attaaactcc gaagcaaatt tcgtaaactc tgtggtggtc tgattatcta aatctttgcg
     1561 gtccactaac atgatcgttc gatcgatccc cgattttcgt gctaaaagct tagtagatac
     1621 aaaactagtc aacgtctttc ctgaaccagt cgcatgccac acataccctg attgatgttt
     1681 atttgcggca ataaacaagg cttgaatcgc atgcacttga taaggatgta acaccatgag
     1741 ggttttattg tcttgatctt cactgacaat cgtataatcc gcaatcaagc gatgggcatc
     1801 tgggatattt agcacttgtt tgcaaaactc atataagttt tctacctttt tattatcttt
     1861 cgttcgccaa ctaaataaaa acttaggatg caaatccttc ggtaaggcat ttgcaaaata
     1921 ccgagtcgtt tgttcattgg aaaccacaaa caactgtaag gtcgaaaaga tattgttccg
     1981 aaacatgcct tcttcagcat attttttgat ttgattaaac gcctgataaa aaccatcttt
     2041 tgcagttact tgttttagct cgatttggat aatcggtaag ccattgatca ataaggtgac
     2101 gtcaaaacgt cgatcccgcg cttctatgtt acttccacgt tttgcaattt gatggaccac
     2161 ttcataagta gagattcctc caccaatgtc ttgattggaa taaaggataa gtgagacaga
     2221 acccaattgc ggattttccc gttcaatcgt gatccgagcc atcccatttt caccttttaa
     2281 ccatttcgca gcatcaaatg gggttttcgt ccgtagcaat aattcggttt tgatcgtttc
     2341 aaactcttta tcggtcaaag gagaatcatt cagttctgct tgattatttg agatgatttt
     2401 ttgacgtaaa ttgctccaaa gatcttcttc tgattttaga tccggacgat acgtccactg
     2461 attatgtcct tccccgagga cacgaatcaa ctgttcttcg acttccgctt cctcacggtg
     2521 actaattttc attcttcttc cctccctaaa caaacatctt ttgcaagaag ccttttttcg
     2581 tttccttcag caaatctaac ttacgttgat gaagagcgat cgtatcatct agctgcttga
     2641 agaaggcacc aattttttgt tgttcattta tatccggtgg aaactgaaat gtcctttgtt
     2701 ctataccacc tttagtcaca tgtaccattg tcgttccgtt agttgattct ttaattttct
     2761 ctttatcagt caatagccaa gtatatagat actgtttgtc caattcctta gataaaaatt
     2821 taagtttcca aatatgataa tggtagatta cacgttcttc attccacatt tcaggaccga
     2881 agcttgttgc ccataaatat aagagatctc ctttattagc atacttgtct tcctctaatt
     2941 ctaagtttga ataataccat ctatcattag tattaaaatt acctactctt aaaaccctat
     3001 attttccaga atcaagtaat tcttgttgtt taaaagctct accattcaaa aaaactacca
     3061 cttcccccaa cttccgctct tcccaatctc ccgtaaatcc tggaaaacga acttctggca
     3121 cttttgctcc gtttttaggg aacatttttt gtaagaatcc ttttttcgtt tccttcagca
     3181 aatctaattc gtgctggtga agcatgatgg tgtgatcaag ttgtttgaaa aatgaaccga
     3241 tcttgatctg ttcgtcataa gttggaagtg agataggtgt ctttttacca ctatcaattg
     3301 tataggttcc gacggttcca atgcctgcaa cgtcacggat atacttctga attttgttat
     3361 ttatgaacga gtagtaaaag aagtttccgt caattttatg ctcattcttg aaccaaatga
     3421 tgtttccatc cttaaagtaa attgggttat catcagtgat taataagggg acacctattg
     3481 atccgacacc agttacaagc aagtctcctt gagaaacttt acctgaattt ttcgaatatt
     3541 cattatattt ttcttctgaa atgtaaagat aatcggatgg ttcctcattt tttgcagctg
     3601 agacaatatc acgagcacgt aaaaatctaa tacctgagtt agtccaatct gattgatgaa
     3661 ttcgcttcac agaagtaaca tccatcatat ctcctaactt acgctcttcc caatcttccg
     3721 taaaaccagg aaaccggatt tctggtcgtg tatcattgct catgtttgaa cacctccagc
     3781 gcaccttgta accacgcttc attttcaggt gttgtttgca gggaagagat agcttctaaa
     3841 agttcttttt ctagcacttg ctgttcttgt cgtatcttct tgatgtcatt tccgacatga
     3901 accatatcaa ttggttcttc ttcttcgaaa gtatcgacat agcgtgggat atttaagtta
     3961 tagtcatttt ctttgatttc atcaaaggtt gccaaatgag cgtatttctc gacatctttt
     4021 ctttcagcgt aggtttcgag gattttttga atgttttctt ccgataattt attctgattt
     4081 ttccctttaa caaattcacg actagcatca atgaataata catctcgagt ttgacgattc
     4141 ttcttcaata caatcaccgt cgttggaatc gatgtaccaa agaacaagtt cgcaggcata
     4201 ccgatcacag cataaatact gccgtcttct aataattttt gacgaatgac cccttctgca
     4261 gcaccacgga ataaaacccc gtgtggcaag acgatagcca tagtccctgt ttccttcaaa
     4321 tgatagaaac catgaagaag aaacgcaaaa tctgctttgg attttggcgc taatttcccg
     4381 tagcgattaa accgagaatc atctaagaag gtcgtatctg ctgaccaatt agccgaataa
     4441 ggtggattca tcactactgc atcaaatgtg taaggttcat ctgttggcca gtccttattc
     4501 aacgtatccc cattgcggag attcatttct tcggcatcta ccccatgtaa aatcaagttc
     4561 atttttgcta gattgtatgt cgttgtattt agttcttgcc catgatattt aacgttatct
     4621 ggatgagtta agtagttacg tacatttaac atcaaggaac ctgaacccat ggttgggtca
     4681 aagacactaa agaatcgtcg ttctttttga tccaacgtca cgatttgtgc catcatatca
     4741 gaaaccatat gaggcgtgta aaattctccg gctttcttcc ctgcttctga agcaaattga
     4801 ctgatcagaa attcatacgc atccccgatc acatccccat cgtgacctaa aacttccacg
     4861 tcattcaatt tcttgataac ttcggtaatc gtcacatttc gttgttgctc atctgttcct
     4921 agtttttttg attggagatc cacatcatca aataacccat taaattgatt gtaggtagat
     4981 gccaattgca caaaggcttt gtttagatcg ttcaattgga acgttgcttg ttttgcttga
     5041 tccgctaaga cgttgaaaag atactctggt gagatcgaat accccaaaat atcaacgatt
     5101 gtggcaatca ggtcttcttt tgattcttca tctgaaagta attcacgata caacattgtt
     5161 tgtttgctta ctgtgtcata ttcctctaag gattcatctg ctaaaagaac cacttgttcc
     5221 aacaaacggt cggataaata tttatagaaa atcagtccca ataaataatt tttatattct
     5281 gacgcatcca ttttactacg gagattatct gccgcactaa ataatttttg atttaattct
     5341 gcacccaaaa agatacactc ctatctctta tttaaagttg ctatataccc tcttatttta
     5401 gcatactccc ttttagaaaa agcaaaaaaa aaagaaaaaa tcttattctt gataacaagc
     5461 tacgtgacgt atatgcctaa caaaaataga acttttgaat caatcttaaa tgaaggttta
     5521 accacagaac cggcatccat tatttttgat gaagttatcg atgacgttga agatagtaag
     5581 tatcataaaa atttaaaaga acttttttat tttgcactca acaatggacc aagtttgttc
     5641 actggtatat cagtacctga tagtgataat ccattaatta tggcaaaaat ttacttaaca
     5701 aaatggtgta ataaatacat cagggatagg aataacccag cattaaaaaa acctcttaaa
     5761 acatttggcg aaaaagatgc tgctttaact actagagtag cagcaaatgc taatattgat
     5821 gatcaaaaag tattgaatga ctacttgcgt ggacattttt tatatatgag cgctgagaac
     5881 atgaacggaa gtattttaga ggaatattta gccgaagtgt tagaacctga aggttggatt
     5941 tggtgcgccg gctctgtata tcgagctgtt gatttctgtt atttaggaac ctcaccaatt
     6001 cttttacaag tgaaaaataa atacaataca gaaagcagtt cgagttcagc tataagagta
     6061 ggaacaacaa ttagaaaatg gaatcgttta aataagtcga caaaaatttc tggtttggat
     6121 agccctatcc ccaattggaa agcattaata gaaatgactg aagctagtaa ggaactggca
     6181 gctaagttaa ctgaaaatag ttatttagct tatattaatg aaaaatccac acgagaatta
     6241 tggacactcg atgactaaca atatccttat tagtatctat ccacgataca tggaaaaaat
     6301 tataaacaaa caaaaaaact ttgaatttag aaatttctat cttacctcag atccttataa
     6361 tatcttttgg atttacgaaa caatccccac caaagcttta aagtataaat tgattgtaaa
     6421 aaatccaatt actaaactta agaaaaatca acactacttt ttaggagacg atgagtttga
     6481 acaattagtt taaaag
//
LOCUS       DACEEZ010000117         6426 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20313_2017
            SAMN12639966-rid9879223.denovo.119, whole genome shotgun sequence.
ACCESSION   DACEEZ010000117 DACEEZ010000000
VERSION     DACEEZ010000117.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639966
            Sequence Read Archive: SRR10023158
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6426)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6426)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741683.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741683.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:44:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,016
            CDSs (total)                      :: 2,962
            Genes (coding)                    :: 2,889
            CDSs (with protein)               :: 2,889
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 36 of 73
            Pseudo Genes (incomplete)         :: 29 of 73
            Pseudo Genes (internal stop)      :: 18 of 73
            Pseudo Genes (multiple problems)  :: 8 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6426
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639966-rid9879223.denovo.119"
                     /isolate="E20313_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(34..714)
                     /locus_tag="IWO89_002610"
     CDS             complement(34..714)
                     /locus_tag="IWO89_002610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS6-like element ISEnfa1 family transposase"
                     /protein_id="HAQ0457309.1"
                     /translation="MTQFKGKQFQKDVIIVAVGYYLRYNLSYREVQEILYDRGIHVCH
                     TTIYRWVQEYGKILYQIWKKKNKQSFYSWKMDETYIKIKGKWHYLYRAIDVDGLTLDI
                     WLRKKRDTQAAYAFLKRLKNQFGEPKVLVTDKAPSIKSAFRKLQKNGLYITTEHRTIK
                     YLNNLIEQDHRPIKRRNKFYQSLRTASTTIKGMEAIRGIYKKSRKEGSLFGFSVCTEI
                     KGLLGIPA"
     gene            758..1582
                     /locus_tag="IWO89_002611"
     CDS             758..1582
                     /locus_tag="IWO89_002611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin zeta"
                     /protein_id="HAQ0457310.1"
                     /translation="MSLSETLQQNPKNRLAVESPTAFLLGGQPGSGKTSLRSAISEET
                     QGNVVIIDNDTFKQQHPNFDELVKLYEKDVVKYVTPYSNRMTEAIISRLRDKGYNLVI
                     EGTGRTTDVPIQTATMLQAKDYETKMYVMAVPKINSYLGTIERYETMYADDPMTARAT
                     PKQAHDIVVKNLPTNLETLHKTGLFSDIRLYNREGVKLYSSLETPSISPKETLERELN
                     RKVSGKEIQPTLERIEQKMVQNQHQETPEFKAIQQKMESLQPPTPPIPKTPKLPGI"
     gene            1704..2201
                     /locus_tag="IWO89_002612"
                     /pseudo
     CDS             1704..2201
                     /locus_tag="IWO89_002612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002405295.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DnaJ domain-containing protein"
     gene            complement(2286..2882)
                     /locus_tag="IWO89_002613"
     CDS             complement(2286..2882)
                     /locus_tag="IWO89_002613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015735562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HAQ0457311.1"
                     /translation="MDGFERRTEEKKKKVLRAAFDLMNTDTGIENLTIDAVAKKANVG
                     KTSIFKYFGSKEKLIHEVFIRSMDEMGEKAKKIMEKNASFEETLIAMSQNKIDYLEKI
                     NKQFYLDLMSYYTKKEDSGFTRMMEAYTKQSFGIMLDLFHRGRKEGKVDLKYSDEFLM
                     LYFQAMIEGISNPQIYEKILPYTAEWTELLVKGIAPRQ"
     gene            3193..4074
                     /locus_tag="IWO89_002614"
     CDS             3193..4074
                     /locus_tag="IWO89_002614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ0457312.1"
                     /translation="MTEIVKVQGLQKKFGKFQALKDVSFTVNAGEVVGFIGPNGAGKS
                     TTIRTLLGIINRDEGDVQIFGKDVWKDSLEIHKRISYVPGDVALWGSLTGGEIIDLFI
                     KLHGGGSKAKRDYLIKRFELDPKKKAKGYSKGNRQKVGLIAALSVESDLYILDEPTSG
                     LDPLMEAVFQEEVEKIKNDGKAILLSSHILSEVERLADKVAIIRRGEVVETGTLDELR
                     HLTRSTVTLVTKGDIEKLATLSGVHDFVQKDGKATFSADNEAMNTILTEATKLGVIKI
                     ESVPPTLEDLFMRHYEG"
     gene            4098..5711
                     /locus_tag="IWO89_002615"
     CDS             4098..5711
                     /locus_tag="IWO89_002615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007128037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetronasin resistance protein"
                     /protein_id="HAQ0457313.1"
                     /translation="MNEKFARWNVLFIQYVKRDWKKIIVWVLGLGLFSGAFVPAFEEI
                     AKGQGLLGMFETMQNPAMISMVGPTPIKIGTDYTLGAMYAQEMLLFCGLFAMIISALH
                     VVSHTRKEEELGLTELVRSFRVGRQANSLAVISEMLLINLLLGLLIGGLMMSFGVKTI
                     DAEGAFLFGGSIALAGIIGGVLALVMSQIMATSTGATGSTLSLIGLLYIVRAGTDVSN
                     LDLSMFNPMGWIYLTYPFTENNWLPLLFALIFSLVFTVLAFVLEEHRDMGAGYLPERE
                     GRATAKKSLLSVPGLFFKINKGVMIGWLIAFVVMGAAYGSIYGDMQVFLGGNELMKQM
                     FTQSGVSIEESFTATIMMVMIGLVTILPIAVVNKLFAEETRLHLSQLYVTKITRGQLY
                     WTTIFLAIFAGVVGIGLASAGLGGTAISAMKNESTMDLTDFLAAGYNFLPSILFYIGL
                     AALALGWLPKFGKVIYAYLGYSFALNYFGGILDLPDWFSKTAIQSWIPRLPMEKFDGT
                     IFAVITVISIVFLFVGYLGYKRRDMVEGA"
     gene            complement(5783..6295)
                     /locus_tag="IWO89_002616"
                     /pseudo
     CDS             complement(5783..6295)
                     /locus_tag="IWO89_002616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337518.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
BASE COUNT         2045 a         1131 c         1254 g         1996 t
ORIGIN      
        1 aaaaggtcat tagtttttca agatgattct tatttaagca gggattccta atagtccttt
       61 gatttctgta cagacggaaa agccaaaaag agacccttct tttcggcttt ttttataaat
      121 tcctcgaatc gcttccatgc ctttaatcgt ggttgaggct gttcgcaaac tttggtaaaa
      181 tttgtttctt cgcttaattg gacgatggtc ttgttcaatc agattattca gatacttgat
      241 tgttcgatgt tctgttgtta tatacagtcc atttttctga agctttctga aggcactctt
      301 aatagagggt gctttatccg ttactagaac ctttggttct ccaaactggt tcttcagtcg
      361 ttttagaaaa gcatacgcag cctgtgtgtc gcgctttttc cgtaaccaga tatctaaggt
      421 taagccatct acatcgatag cgcgatataa ataatgccac tttcctttaa ttttgatata
      481 agtctcgtcc attttccatg aataaaagga ctgtttgttt ttctttttcc aaatttgata
      541 aagaatcttt ccgtattctt gcacccaacg gtaaattgtg gtgtgacaaa cgtgaattcc
      601 tcggtcatac agaatttcct gcacttcacg ataactcagg ttgtaccgaa gatagtagcc
      661 gacggctacg ataatcacgt ctttttgaaa ttgttttcct ttgaactgag tcatcttcat
      721 tcctccaagc tatctttttc aattatttta cctcaaaatg agcttatcgg aaactttgca
      781 acagaaccct aaaaatagac tagcggttga atcgccaacc gcttttttac ttggtgggca
      841 accaggatca ggtaaaacta gtttgcgatc ggcaatttcc gaagagacac aaggaaatgt
      901 tgttatcatt gataatgata cgttcaaaca acagcaccct aattttgatg aactagtgaa
      961 actttatgaa aaagacgtag taaaatacgt taccccttat tctaatcgca tgacagaagc
     1021 gatcataagc cgtttgagag ataaagggta taatttagtg attgaaggta caggacgaac
     1081 aacagacgtt cctattcaaa ccgcaacaat gcttcaagcc aaagattatg aaacaaaaat
     1141 gtatgtcatg gcagtaccta aaatcaactc atatttagga acaattgaac ggtatgaaac
     1201 tatgtatgca gatgatccaa tgacagccag ggcaacacca aaacaagcgc atgatattgt
     1261 tgtcaaaaac ttaccgacca atttagaaac ccttcataaa acgggcttat ttagcgatat
     1321 aaggctttac aatagagaag gagtgaaact ctattcaagc ttagaaacgc cttccattag
     1381 tccgaaagag accttagaaa gagaattaaa tcgtaaagta tcagggaaag aaattcaacc
     1441 gactttggag agaatagagc aaaaaatggt tcaaaatcaa caccaagaaa cccctgaatt
     1501 taaagcaatt caacaaaaaa tggaaagctt gcagccacct acaccaccaa tacccaaaac
     1561 acctaaactt ccaggtattt aaaccttaaa aaaggaaaag agtagttacc aaaaaacggt
     1621 aactactctt tttttataaa aacatttcct caaacgccta acaaaaatgg ttgtcaaaaa
     1681 gccaaaaatc aaaaagaaaa ggaatgataa aaatgaaata cattaaaaac gttgaaacat
     1741 tggaagaatt gaaaaaagcc tacaaaaagt tagctttgaa gttacacccc gattgtggag
     1801 ggaatgaaga agaaatgaaa actttgaaca atgaatatga cgaactgttt agcaaactta
     1861 aaaacactca taagaataaa gacggtgaaa cttacaccaa agaaacaaca gaaacacccg
     1921 aacagtttaa ggatattatt aataaattgt tcaatctaaa aatggacggc gtagcaatcg
     1981 aagtagtagg aacatttata tgactaacag gtaatacaaa accatataaa gaagatataa
     2041 aagccctaga attccgatat tcacctaaaa aatatgcttg gtataaagcc cctaaagatt
     2101 acaaaaaaag aagccgacta cgatcaatct gtgagaagcg gctgtctctg agacaattgc
     2161 atcacatttt agttgtgaat atgtatctca aatacaccta gcagatgaat cgattctgat
     2221 ttgtatgcat acattttatt tttttgtgga ttcggcgagt atttcttctt ttgttgttcg
     2281 taaaactatt gtctcggagc gattcctttt accaacagct cagtccattc cgctgtataa
     2341 ggcaggattt tctcataaat ctgcggatta gagatgcctt cgatcatggc ttgaaaatac
     2401 aacatcaaaa attcatctga atattttaaa tcgacttttc cttctttacg cccgcgatgg
     2461 aataaatcca acataatgcc aaaactttgc tttgtataag cttccatcat tcgggtaaag
     2521 ccgctgtctt cttttttagt gtaatagctc attaaatcta ggtaaaattg tttattgatt
     2581 ttttcgaggt aatcgatttt attttgactc atggcgatca aagtttcttc aaatgatgca
     2641 tttttctcca tgattttttt agctttttcc cccatttcat ccattgaccg gataaagact
     2701 tcatgaatga gcttttcttt gctgccaaaa tatttaaaaa tagaagtttt tcctacattg
     2761 gcctttttgg ctacggcatc gatcgttaaa ttctcgattc ccgtatctgt attcatcaga
     2821 tcaaaagcag ctcgtaaaac ttttttcttt ttttcttctg ttcttcgttc aaaaccatcc
     2881 attaaaaata cctactttct ataaaaaaga actttttgtt tatcatagtt caaaaatagc
     2941 ttcgtatata ttatatagga aataatacga actaacaatg gaatcatagg tttaaattta
     3001 aaattatttt tattgactgt taaaataaaa aagaatatta ttggagctgt aaaagaactt
     3061 tggtttgaaa caaagtataa aggggtattt tatgaacgat acgtattgta ttggttcata
     3121 aaaaaggtgc gttttgttca gactggaaag ctcgttataa aatcatgtca aaagaaaagg
     3181 gagttatgta ccatgacaga aattgtaaaa gtacaaggct tgcaaaaaaa atttggtaaa
     3241 ttccaggcgt tgaaagatgt ctcattcaca gtaaacgccg gtgaagttgt tggttttatc
     3301 ggaccaaatg gagcaggaaa gtcaacgacg attcgtacac tgctaggaat catcaaccga
     3361 gacgaaggag atgtccaaat attcggaaaa gatgtttgga aagatagtct agaaatccat
     3421 aaacgaattt cgtatgttcc tggggatgtt gctctttggg gcagcctgac tggtggagag
     3481 atcattgatc tatttatcaa acttcatggc ggcgggagca aagcaaagcg tgattattta
     3541 atcaaacgat ttgaacttga tccaaagaaa aaagccaaag gttactctaa aggaaatcgt
     3601 caaaaagtcg gtttgattgc tgcactttca gttgaatctg atctgtatat tttagatgaa
     3661 ccgacttcag gactagatcc attgatggaa gcagtattcc aagaagaagt agaaaaaatc
     3721 aaaaatgatg gcaaagcgat tctattatct tcacatattt taagtgaagt tgaacgatta
     3781 gcagataaag tagcaatcat tcgacgtgga gaagtagttg aaacaggtac attagatgaa
     3841 ttgcgtcatt tgactcgctc aacagttaca ttggtgacaa aaggcgatat tgagaaactt
     3901 gcgacgctct ctggcgtgca tgattttgtt caaaaagacg gcaaagcaac tttttctgct
     3961 gacaatgaag cgatgaatac gattctgacc gaggcaacca aattaggtgt gataaaaatc
     4021 gaatctgtac cgccaacgct cgaagattta ttcatgcgtc actacgaagg ctgattgtcg
     4081 gaacggagga aaagaaaatg aatgaaaaat ttgcgcgttg gaacgtattg ttcattcaat
     4141 acgtgaaacg cgattggaaa aaaataattg tttgggtttt aggtttgggt ttgttctcag
     4201 gagcatttgt accagcattt gaagagattg ctaaaggaca aggtctttta gggatgtttg
     4261 aaacgatgca aaatccagcg atgatctcga tggttggacc tacaccaatc aaaataggta
     4321 cggattatac tttaggagcg atgtatgctc aagagatgtt gctgttttgc ggattgttcg
     4381 caatgattat ctcagcactt catgtggtga gccacacgcg aaaagaagaa gaattaggtt
     4441 tgactgaatt ggttcgctca tttcgagtag gacgacaagc caattcatta gctgttatca
     4501 gtgagatgct gttgatcaat cttttattag gtcttttaat cggcggactc atgatgagtt
     4561 ttggtgtaaa aacgattgat gccgaaggag ctttcttgtt cggaggatca attgcattgg
     4621 cgggaattat cggtggtgta ttggcacttg tgatgtcgca gattatggcg acttctactg
     4681 gagcaaccgg ctcgacatta agtcttatag gacttttgta tatcgtgcgc gctggaacag
     4741 atgtgtctaa tcttgattta tcaatgttca atccaatggg atggatttac ttgacctatc
     4801 ctttcacaga aaataactgg ctaccattat tatttgcttt gatttttagt cttgttttta
     4861 ccgtacttgc gtttgtgttg gaagaacatc gcgacatggg cgcaggttat cttcctgaac
     4921 gagaaggacg tgcgacggcg aagaaatcac tactttctgt acctggtttg tttttcaaga
     4981 ttaataaagg agtaatgatt ggttggctga tcgcatttgt cgttatggga gctgcgtatg
     5041 gctccattta tggagacatg caagtctttc ttggcggaaa tgaactgatg aaacaaatgt
     5101 tcactcaatc tggcgtttcc attgaagaat cctttacggc aacgatcatg atggtaatga
     5161 ttggattagt cacaatcttg ccaatcgcgg tggtcaataa attatttgca gaagaaacaa
     5221 gactgcatct gagtcaactg tatgtaacga agattacgcg aggccaatta tattggacaa
     5281 cgatattttt agctattttt gctggagtcg taggtattgg cttagcatca gcgggattag
     5341 gtggaacggc gatttctgcg atgaaaaatg aatcgactat ggatctgacc gatttcttag
     5401 ctgctggata caattttctc ccttccatct tattttatat tggtttggct gctttagcgt
     5461 taggctggtt gccaaaattt ggaaaagtaa tctatgctta tctaggctat tcctttgctt
     5521 tgaattattt cggcggaatc ttagatttgc cggattggtt ctcaaaaact gcaattcaaa
     5581 gttggattcc acgcttaccg atggaaaaat ttgatggaac gatttttgca gtaattactg
     5641 ttatcagtat cgtcttctta tttgtcggct atttaggata caaacgccgt gatatggtag
     5701 aaggcgctta aaaattgaat ttctaaaaaa ccgttcatat ttacgaaagt cgatttgagc
     5761 ggttttttta tggcttcaat tgtcaaatta agtgcaacga ttcattaaaa aatacttcat
     5821 aaggggtttt ccaatttaga catttccgtg gtcgcgtgtt taatttattc gcccattctt
     5881 gaatttgact atcgaaaata cctgaaatat cttcaccttt gggtgaatat tctcgtaata
     5941 aaccattagt attttcgttt gtaccacgtt gccatggagc atgtggatct ggaaaataca
     6001 ctttagtgcc atttaattct agcgttattc gactatgttg ggagaattct tttcctctat
     6061 cgggtgtaac ggttttacat ttatcttctc ctacatttct tagtagattg atcatacaat
     6121 caaccacatt tttagagatt ttcttttcaa ccttaccaag tagggagaaa cgagatctac
     6181 gatcgactag agttacaata cgagccctac ctgttttacc ggctacagta tcagcttccc
     6241 aattaccaat ctcaaggcgc tcattggcag cagatggcct ttcatggatc ttattgtatg
     6301 cagatgatcc aatgacagct agggcaccac caaaacaagc gcatgatatt gttgtcaaaa
     6361 acttaccgac caatttagaa acccttcata aaacgggctt atttagcgat ataaggcttt
     6421 acaata
//
LOCUS       DACEEZ010000118         6401 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20313_2017
            SAMN12639966-rid9879223.denovo.120, whole genome shotgun sequence.
ACCESSION   DACEEZ010000118 DACEEZ010000000
VERSION     DACEEZ010000118.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639966
            Sequence Read Archive: SRR10023158
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6401)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6401)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741683.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741683.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:44:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,016
            CDSs (total)                      :: 2,962
            Genes (coding)                    :: 2,889
            CDSs (with protein)               :: 2,889
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 36 of 73
            Pseudo Genes (incomplete)         :: 29 of 73
            Pseudo Genes (internal stop)      :: 18 of 73
            Pseudo Genes (multiple problems)  :: 8 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6401
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639966-rid9879223.denovo.120"
                     /isolate="E20313_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            <1..177
                     /locus_tag="IWO89_002617"
     CDS             <1..177
                     /locus_tag="IWO89_002617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002570387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0457314.1"
                     /translation="QSLVLLAIVVIGLFLGFKREFSKLIGFLVIALIAVGLVFNASGV
                     KDVLLNLFNRIVGA"
     gene            190..690
                     /locus_tag="IWO89_002618"
     CDS             190..690
                     /locus_tag="IWO89_002618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein ArdA"
                     /protein_id="HAQ0457315.1"
                     /translation="MEQIRVYIANLGKYNEGELVGAWFTPPVDFDEVKERIGLNDEYE
                     EYAIHDYELPFEIDEYTPIEEINRLCGLAEELKGTPIGEVASEIQHAFFNSFEEMVEH
                     VDDIIYYPDCNDMSDVAYYLIVEAGDLGEVPEHLKTYIDFDACGRDLEIGGNYLVTSR
                     GVFELM"
     gene            787..2634
                     /locus_tag="IWO89_002619"
     CDS             787..2634
                     /locus_tag="IWO89_002619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HAQ0457316.1"
                     /translation="MVGNKWYKCDLHIHSTASQCFQDKKVTAKEWVEECLNKGLDCVA
                     LTDHNTGENIDEYKKVAIENGLDFFPGVELTCGENGTHLLVLFDKSACTNDISDFLTK
                     MGIKREDWASAAACSMKSVLEVIDFAEHEGLLVIPAHIDEFNGISSAKFGNLGKIFES
                     ENIYAVQTVQKEFFENRSQMIPSSKRDTIYDSVNARYDGRVGKDTLESWYKSVVEAEK
                     NNMTFLSFSDNPHSKGNSKHGLWGIGTRYSYIKMKDEPDLGSLRDALMLGETRVHSDF
                     SNFSINENEVLLEKLSFSGTTLSTKEVVVEFSDNLTSIIGGRGTGKSCITRFLVYVLG
                     KEAELDQFSEIQSDYQNFAQIEHNGSGIFLKDTIVKLNIFYKGTKYQIIRTQDRHSIY
                     EFTQENGLVEKETERLRMISDKVSIYSQKQIYEISKNQTSILELLDGYNSDLISEYKN
                     EIETCVNEIRKLNWDIVSVKKEIQDKAKVELEIEDLKKQVEKLSHKSYKDVYDEYSKE
                     TDIYRELKRDVEALKEIPKNLTDSVDKIDFGNGIKVTDEIDEHRGELIKNLVTNRAKI
                     QTIINEMSDEIDSYRAELNKSDWKVNYNEVSKRYQRGIKNLVKSYLKMN"
     gene            2637..3533
                     /locus_tag="IWO89_002620"
     CDS             2637..3533
                     /locus_tag="IWO89_002620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HAQ0457317.1"
                     /translation="MLQNLDSLYQSLSKTEERLEEIIQQEIKLKDYQVEVDSAMSRMN
                     KSYQNLYSERSSSSREIFSDITGIEATIQPNRDFNNYVKELRACINKEDTFDSEFGSL
                     IDKLTKNSITNQDIYNDILDIRVSGESSIFSDKKLINSIRKLNDAQVLELQTLKPNDL
                     VVIRLELNGKKVSLSNASAGQKTSAILSMILAYGNSPLILDQPEDDLDSQLINNLIVR
                     SIITKKENRQILIVTHNANIPVNGDSEWLISMGDSKEISADQCGSVDEPNIKPRICTV
                     MEGGEDAFTNRAKRYGFKQIVE"
     gene            3583..3972
                     /locus_tag="IWO89_002621"
     CDS             3583..3972
                     /locus_tag="IWO89_002621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HAQ0457318.1"
                     /translation="MKKIKSYTSIWAVEKVIYAINDFQLPFPVTFNQMAWFVLSLLFV
                     IVFAHVPPLSMIEEVFLKYLGIPVAVTWFMSQKTFDGKKPLGFLRSFISYHLRFKVTF
                     AGKKVKEQKKRWDEPSTLVRSVNYVSD"
     gene            3959..6304
                     /locus_tag="IWO89_002622"
     CDS             3959..6304
                     /locus_tag="IWO89_002622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HAQ0457319.1"
                     /translation="MYPIKYIENNLVFNQEGECFAYYELVPYNYSFLSPEQKFQVHDN
                     FRQLIAQNREGKIHALQIATESSIRATQERSKKEITGRLKEVAKQRIDIQTDALVSMI
                     GDSQIDYRFFIGFKLIATDEEVNLKSLKKSFFSGLQEFVYGVNHHLMGDFVSLSNEEI
                     RCYSKLEKLMESKLARRFKVRRVTPSDLTYLIEHIYGEKGIPFEEYEFQLPKKKLKSE
                     TLVKRYDLLRPNRCLIEEKPRCLRMEHENHESYVAYLTINTIVGEMEFPSSELFYYQQ
                     QQFTFPIDTSMNVEIVTNKKALATVRNKKKELKDLDNHAYQSDNETNSNVLDALDSVD
                     ELETTLDQSKESMYKLSYVVRVSAESVDELKRRCDEVLDFYDDTNVKLVRPFGDMMGL
                     HEEFLPSSKRYMNDYIQYVTSDFLAGLGFGATQMLGELEGIYFGYNVDTGRNVYLKPA
                     LASQGVKGSVTNALAAAFLGSLGGGKSFSNNLLVYYAVLFGGQAVIVDPKGERGGWKE
                     TLPEIADEISILNLTSKSENQGLLDPYVIMKKKKDSESLAIDILTFLTGISSRDGEKF
                     PVLRKAIRTVTQSDQRGLLQVINELRKDGSPIAENIADHIESFTDYDFAHLLFSDGSI
                     TQSISLDKQLNIIQVADLVLPDAETTFEEYTTMELLSVSMLIVISTFALDFIHSNREI
                     FKIVDLDEAWSFLQVAQGKTLSNKLVRAGRAMNAGVYFVTQNSDDLLDERLKNNIGLK
                     FAFRSTDIHEIKKTLEFFGLDQEDQSNQKRLRDLENGITSI"
BASE COUNT         2161 a          950 c         1370 g         1920 t
ORIGIN      
        1 caatctttag tcttactagc gattgtggtc attggtttgt ttttaggatt taaacgcgag
       61 ttttccaaac tgattggctt cttggtgatt gctttgattg cggtaggtct ggtctttaat
      121 gcttctggtg tgaaggatgt cttactcaat ttgtttaacc gaattgtcgg tgcctgaggg
      181 ggaatcacaa tggaacagat acgtgtttat attgcgaacc ttggaaaata caatgaaggt
      241 gagttggtgg gggcatggtt taccccaccc gtcgattttg atgaagtgaa agaacggatt
      301 ggattaaatg acgaatatga agagtacgca attcatgatt atgaactgcc ttttgagatt
      361 gatgagtata caccaattga ggaaattaat cggttgtgtg gtttagcgga agagcttaaa
      421 ggaacgccaa ttggtgaggt cgcttcagag attcaacatg ccttcttcaa ttctttcgag
      481 gaaatggtag agcatgtgga tgatatcatc tattatcccg attgtaatga catgagtgat
      541 gtagcgtatt acttgattgt agaagctggt gatttaggcg aagtaccaga acacttgaag
      601 acgtatattg attttgatgc gtgtggtcga gacttggaga ttggcgggaa ttatttggtt
      661 acgagtcgag gggtttttga actaatgtaa atcatttgtt agaattgacc agaaatttct
      721 atcgtacttt tacttttcta tttgatatac tgaaatagaa aatatttgtt gataggggtg
      781 ttactaatgg ttggcaataa gtggtacaaa tgtgatcttc atattcattc aactgcgagt
      841 cagtgctttc aggataaaaa ggttactgct aaggaatggg tagaagaatg tttaaacaaa
      901 ggactcgatt gtgttgcatt aactgatcac aatacagggg aaaatattga tgaatataag
      961 aaagtggcta tcgaaaatgg cttagatttt tttcctggag tggaattaac gtgtggagaa
     1021 aatggaactc atttactagt cctatttgat aaaagtgcat gtacaaatga tatctctgat
     1081 ttcttaacca agatgggtat aaaaagagag gattgggcca gcgctgcagc ttgctcaatg
     1141 aaatcagttt tagaagttat tgattttgct gagcacgagg gcttgctcgt tattcctgca
     1201 catatagatg agtttaatgg aatttcttca gcaaaatttg gaaatctagg aaagatattt
     1261 gaatcagaaa atatatatgc agtacagacg gttcaaaaag aattttttga aaatcgctca
     1321 caaatgattc cctcatctaa gcgtgacact atctatgata gtgtgaatgc cagatatgat
     1381 ggtagagttg gaaaagatac tttggaatct tggtataaat cagttgttga ggcagaaaaa
     1441 aataatatga catttcttag cttttctgat aacccacact cgaaaggaaa ttcaaaacat
     1501 ggactttggg gaataggcac aagatattcc tatataaaaa tgaaggacga acctgacttg
     1561 ggatcacttc gcgatgcttt aatgctaggc gaaactcgag ttcattctga tttttctaat
     1621 ttctccatta atgaaaatga agttttattg gaaaaattaa gcttttctgg gaccactctt
     1681 agcacaaaag aagttgtagt cgaattcagc gataatttaa cttcaattat tggaggaaga
     1741 ggaacaggca aatcttgtat tacaagattc ttggtctatg tccttggaaa ggaagcagaa
     1801 cttgatcaat tttctgaaat acaaagtgat tatcagaact ttgcacaaat tgaacataat
     1861 ggctctggaa tatttttgaa ggatacaatt gtgaaactga atattttcta taaggggaca
     1921 aaatatcaaa taataagaac acaggataga cattctattt atgaatttac acaagagaat
     1981 ggtctggttg aaaaagaaac agaacggttg agaatgattt ccgataaggt gagtatatac
     2041 tctcaaaaac aaatttatga aatttcaaaa aatcaaacta gtatactaga attactagat
     2101 ggatataact ctgatttgat ttcagagtat aaaaatgaga ttgaaacatg tgtaaacgaa
     2161 ataagaaaat taaattggga tattgtttct gtgaaaaaag aaatacaaga taaggcgaag
     2221 gtagagcttg aaattgaaga tttaaaaaaa caagtagaaa agctatctca taagagttat
     2281 aaagatgttt atgatgagta ttcaaaagag acagatattt atagggaact gaaacgtgat
     2341 gtggaggcac ttaaggaaat tcccaaaaat ctaactgata gtgttgacaa aattgacttt
     2401 ggcaatggta taaaagttac agacgaaatt gatgaacacc gcggagaatt gattaagaat
     2461 ttagtgacca atcgggctaa gattcaaaca ataattaatg aaatgtcaga tgaaattgat
     2521 agttatagag cagagctgaa taaatcagat tggaaagtta actataatga ggtttcaaag
     2581 aggtatcaaa gaggtatcaa aaacttagtg aagagttatc tcaagatgaa ttaaaaatgt
     2641 tacaaaacct tgattcttta tatcaatctt tatcaaaaac tgaagagcga ttggaagaaa
     2701 ttattcaaca agaaataaag cttaaagatt accaagttga ggttgactca gcaatgtcaa
     2761 gaatgaataa atcatatcaa aatctctatt ctgagagaag tagttcctcg cgcgaaattt
     2821 tttctgatat tacaggaata gaggctacta ttcaaccaaa tagggatttc aataattatg
     2881 ttaaagagct cagagcttgt attaataagg aggacacttt tgacagtgag tttggatctc
     2941 ttattgataa gctgacaaaa aattccatta ctaatcaaga tatatataat gacatacttg
     3001 atataagagt tagtggggaa agtagtattt tttcagataa aaaattaatc aattctataa
     3061 gaaaattgaa tgatgcacaa gttcttgaat tacaaaccct taagccaaat gatttagtag
     3121 ttatccgact tgaactaaat ggaaaaaaag ttagtttatc gaatgctagt gctggtcaaa
     3181 agacatctgc aattctttct atgattttgg cgtatggaaa ttcaccactc atattggatc
     3241 agcctgaaga tgatttggat agtcaattaa ttaataactt aatcgtaaga agtatcatta
     3301 caaaaaagga aaacagacaa attttaattg taactcataa tgcaaatatc ccagttaatg
     3361 gtgattcaga atggttgatt agcatgggag attcaaaaga aatatcggct gatcagtgcg
     3421 ggtcggtaga tgaacccaat atcaagccaa gaatatgtac tgtgatggaa ggtggcgagg
     3481 atgcttttac aaatagagcg aaacgctatg gttttaaaca aattgtagaa taacactaga
     3541 gggtgagaca tattattatg tccgcccttt ttaggaggaa aaatgaaaaa aataaaaagc
     3601 tataccagta tctgggcagt tgaaaaagtc atttacgcaa tcaatgattt tcagttgcct
     3661 tttcctgtga cttttaacca aatggcttgg ttcgttttat ctttactgtt tgtcatcgta
     3721 tttgcccatg tgcctccact atctatgata gaggaggtct ttttaaaata cttaggcata
     3781 ccggtggctg tcacttggtt tatgtcgcaa aagacgtttg atggcaagaa accactggga
     3841 tttttacgat cctttatcag ctatcacttg cgatttaagg tgacttttgc aggaaagaaa
     3901 gtcaaagaac agaagaaacg atgggatgaa cccagtactc ttgtaaggag tgtgaactat
     3961 gtatccgatt aagtatattg aaaataatct cgtctttaac caagaaggcg agtgttttgc
     4021 ctactatgaa ttagtgcctt acaattactc ctttttgtca cccgaacaga aatttcaagt
     4081 gcatgataac tttcgccaac taattgcgca aaatcgtgaa ggcaagatcc atgcgttaca
     4141 aattgccaca gaaagtagta ttcgcgccac acaagaacga tcaaaaaaag aaattaccgg
     4201 tagactcaaa gaagtagcga aacaacgaat tgatatacag acagatgcat tagtatccat
     4261 gattggcgat agtcagattg attatcgctt ttttattggc tttaaactaa ttgcgacaga
     4321 tgaagaagtc aatctaaaga gtctcaagaa atctttcttt tctgggcttc aagaatttgt
     4381 ctatggagtg aatcatcatt tgatgggcga ttttgtttcc ttatccaatg aagaaattcg
     4441 ctgctattct aagcttgaaa aattaatgga aagcaaattg gctcgaagat ttaaagtcag
     4501 gcgagtcaca ccgagtgacc tgacgtattt gattgaacat atttatggcg aaaaagggat
     4561 accgtttgaa gaatacgaat ttcagctacc aaagaaaaag ctaaaatcag aaacactggt
     4621 gaaacgttat gacttattac gtccaaatcg ttgtttaatt gaagaaaaac caaggtgctt
     4681 acggatggaa catgagaatc atgagtcgta cgtagcgtat ttaacaatta atacaattgt
     4741 gggggagatg gaatttcctt cctcagaact tttctattac cagcaacaac aatttacatt
     4801 cccaatcgac acaagtatga atgtagagat tgtgacgaat aaaaaggctt tagctaccgt
     4861 tcgtaataag aaaaaagaac tgaaagattt agacaatcat gcttaccaat ctgacaatga
     4921 aacgaattcc aatgtcttag atgctttgga ttccgtggat gaattggaaa ccaccttgga
     4981 tcagtcaaaa gaatctatgt acaaactgag ttatgtggtt cgagtgagtg ccgaatcagt
     5041 ggatgaacta aagcgacgct gtgatgaagt gctcgatttt tatgatgata ccaatgtaaa
     5101 actagtccgg ccctttggcg atatgatggg gctacatgaa gaattcttac cctcaagtaa
     5161 acgatatatg aacgactata ttcagtatgt tacgtctgat tttttggcgg ggcttggttt
     5221 tggggctact cagatgctag gagaattaga agggatttac tttggctaca atgtggatac
     5281 aggtcgtaat gtttacctga aaccggcatt ggcttcacaa ggagtcaagg gttcagtaac
     5341 caatgcgctg gccgccgctt tcctgggttc tcttggtgga ggtaaatcct ttagtaacaa
     5401 tctcttagtc tactatgctg ttttgttcgg tggtcaagct gtgattgtcg atccaaaagg
     5461 ggagcgagga gggtggaaag aaaccttgcc agaaattgcg gatgaaatca gtatcctcaa
     5521 cttgacgagt aagtcagaaa atcaaggtct gcttgatcct tatgtgatta tgaagaaaaa
     5581 gaaagactcg gaaagtttag caattgatat tctgactttt ttaacaggaa tttcaagtcg
     5641 agacggggag aagtttcctg tgttacgtaa agccattcga acggtgactc aaagtgatca
     5701 acgggggtta ttacaagtta ttaacgaatt gcgaaaagac gggtcaccaa tcgcggaaaa
     5761 tatcgccgat catatcgagt cctttaccga ttatgatttt gctcatttgc ttttctcaga
     5821 cggctcgatt acccaatcga ttagtttaga taaacagcta aatatcattc aagtggcgga
     5881 tttggtttta ccggatgcag aaacgacttt tgaagaatat accaccatgg aattgcttag
     5941 tgtttcgatg ctaatcgtca tttccacttt tgccttggac tttatccata gtaaccgaga
     6001 aatcttcaaa attgtcgatt tggatgaggc ttggagtttt cttcaagtgg ctcaaggaaa
     6061 aacgttatcg aataagcttg ttcgtgctgg tcgggccatg aatgctgggg tctatttcgt
     6121 cactcaaaat agcgacgatt tattagatga acgtttaaaa aataatatcg ggttgaagtt
     6181 tgcttttcgc agtaccgata ttcatgaaat caagaaaacc ttggaattct ttggtttgga
     6241 tcaggaggat caaagcaatc aaaaacgcct gcgagatttg gaaaacggga ttacgtccat
     6301 ataattgtgt aaaagtgaag catcaaaaaa gccattgatc tcagttagga gtacaatgtt
     6361 ttttgcagaa aacatactcg aataggactg atatcaatga c
//
LOCUS       DACEEZ010000119         6303 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20313_2017
            SAMN12639966-rid9879223.denovo.121, whole genome shotgun sequence.
ACCESSION   DACEEZ010000119 DACEEZ010000000
VERSION     DACEEZ010000119.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639966
            Sequence Read Archive: SRR10023158
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6303)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6303)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741683.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741683.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:44:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,016
            CDSs (total)                      :: 2,962
            Genes (coding)                    :: 2,889
            CDSs (with protein)               :: 2,889
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 36 of 73
            Pseudo Genes (incomplete)         :: 29 of 73
            Pseudo Genes (internal stop)      :: 18 of 73
            Pseudo Genes (multiple problems)  :: 8 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6303
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639966-rid9879223.denovo.121"
                     /isolate="E20313_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            334..714
                     /locus_tag="IWO89_002623"
     CDS             334..714
                     /locus_tag="IWO89_002623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="HAQ0457320.1"
                     /translation="MSEQHQNLASDHSKKYTYRSEPKQISFRVSESEFAKLKQSAEAL
                     QMSVPAFVKAKAQNARLVTPKVAPDIAQAIARDLAKAGGNINQIAKWCNTHQHDVAPD
                     DAQRLSENLKIMQKELQKIWQQLK"
     gene            696..1610
                     /locus_tag="IWO89_002624"
     CDS             696..1610
                     /locus_tag="IWO89_002624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /protein_id="HAQ0457321.1"
                     /translation="MATVKVSRTTSCSRAINYAEPRATVKTGINCDIDYAKSEMKQIR
                     TLYGKDDRVQAHLLIQSFRPGEITAEKANQLGAEYAKKIAPNHQIAIYTHTDKDHIHN
                     HIVINSVNLETGKKFQAHGQAFLNKCYDLNDEICLEHDLTITERGKKTEKRTMSEIKL
                     KEKNEPVWKDEIRFAIDQTMKNKKTRTYTQFCDSLKTFGIHCLNRGKNFTYELVHKKK
                     KVRSNKLGKDYEKETILHELDRRERTYNQQRTNELRRARASIRETAEEVRREQRTFTP
                     EQSEIRPTRRNHEQHVERGTDHQQELSL"
     gene            1855..2544
                     /locus_tag="IWO89_002625"
     CDS             1855..2544
                     /locus_tag="IWO89_002625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0457322.1"
                     /translation="MAFYLPYLLISASILGIIWLSYKAYQTRYSLKGSKIRFKRFLLV
                     GVIFALSIVLSGLFGVLEGNKRVSRSILLGDITQKYEAACDKKKKEQALAQKMEEFTA
                     CYEDMNNIFVDQEKRLTDKNMKKLTRFYQNLPEKQQKEVQEKYEQVKKDVQYFKDTQT
                     EEACCNLFSDTIPFSTSEQERKERQQSVTYERYETLLQQATNIQNPTKKETALNYLES
                     VKEWLDQQQQN"
     gene            complement(2665..2751)
                     /locus_tag="IWO89_002626"
     CDS             complement(2665..2751)
                     /locus_tag="IWO89_002626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011302749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Fst family toxin"
                     /protein_id="HAQ0457323.1"
                     /translation="MFERFICPLLVEVCVSLFSYWLNNRNKK"
     gene            3427..4377
                     /locus_tag="IWO89_002627"
     CDS             3427..4377
                     /locus_tag="IWO89_002627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RepB family plasmid replication initiator
                     protein"
                     /protein_id="HAQ0457324.1"
                     /translation="MANEIVKYHHELNTIPLRKFTPVEMNLFFSIVSRMREKGDKTVR
                     LTFDQLKDLSNYKATANNRFINDLEMTYDKLMDLRFGKRSANGLDIDRFVMFSEFKIR
                     GTAKDPYVDIQVYEKALPLLNNLDEWVRYSLQQFNELQSSYAKTMFRLLKQYRTKGFA
                     YFSKEDFHELLDIPKSYWNNQANVDNRVIKPIRQELTAIFKGLSIKKKYGKGRGKPVV
                     GYQFTFKPEIKKADDFNKHAPTKEEQNKALALADKQKEYEEPATTINEKIKQKKRELE
                     LLQQIKQLEKEKEALEKQTESALSKKEKKSLLSDLDNLFQ"
     gene            4398..4931
                     /locus_tag="IWO89_002628"
     CDS             4398..4931
                     /locus_tag="IWO89_002628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF536 domain-containing protein"
                     /protein_id="HAQ0457325.1"
                     /translation="MQPQETFTIKELSELFKMSRQAISKHIQKLDSSMIAKNERGYKV
                     VLRSGVLQLARNLGNQMLLDRLHEYEQDNDKNVNIPSKNVVTNKLLDQLREENEFLRE
                     QLALREQNNSHIQKLLDQQQQLTLQANKQIEKLQEKLQLTYTEEAPENDSTTLSEATE
                     TIDKQIDTEKKWWQLWK"
     gene            5567..6100
                     /locus_tag="IWO89_002629"
     CDS             5567..6100
                     /locus_tag="IWO89_002629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0457326.1"
                     /translation="MKKFTLGVALLTIGAVVLPSTTIVYASENSQVVSLNENNATENV
                     VIEYTEDGMVVTKYEDGYSAEDFANSSDIMTRAAVARWGKKEYTNIAVTTGVAANAIN
                     AAFWAGGKASTSMFGIPGWAIGALLTGASWTKKGASPGNAVAKEWDKNGNGWIGFYYQ
                     RGYDAAGRVVATRYSTL"
BASE COUNT         2324 a         1102 c         1173 g         1704 t
ORIGIN      
        1 aaaaaaaaat aaatcgatac attttagaag aatagggttg tttgaatatt acaaagcaac
       61 aatttcacct tcggtgaaat aagcgaaact ttacaaaaag tggctagcaa atgctagcct
      121 ttttttgtat tgtggagcgt ggattttgag ggggattcaa gggggagctt gcgctccttt
      181 gacaagcggt tatttgcacc aaatacggag gtatttggct agcaaatata gagcttgcca
      241 aaccctttta gtgagcttta ctcattgtac ctttttaggt acaatggtat tgcgaacgat
      301 acgtccgtag gacgtactga aagggtggga cttatgagcg aacaacatca aaatttggct
      361 agcgatcatt ctaaaaaata tacctatcga tccgagccaa aacaaatcag ttttcgagtg
      421 agcgaatccg aatttgcaaa gctgaagcag tcagctgaag ctttgcaaat gagtgtgccg
      481 gctttcgtga aagccaaggc acaaaacgcg cgtctggtga caccgaaagt ggcgccagac
      541 attgcccagg caatcgcgcg cgatttagcc aaagctggag ggaatatcaa tcaaattgcc
      601 aagtggtgca atacacacca acacgacgtt gctcctgatg atgctcaacg tttatccgaa
      661 aacctaaaaa tcatgcaaaa ggagctacaa aagatatggc aacagttaaa gtaagtcgaa
      721 ccacttcatg tagccgagca atcaattacg cagaaccacg agcaaccgtc aaaacaggaa
      781 taaactgtga cattgattat gccaaaagtg aaatgaaaca aatacgcacg ctttatggaa
      841 aagacgatcg tgtacaagca cacttattga ttcagtcctt tcgaccagga gaaattaccg
      901 ctgaaaaagc caatcaacta ggggcagagt atgcgaaaaa aatagcccct aaccaccaaa
      961 tcgcgattta cacacataca gacaaagacc atattcataa tcacattgtc attaactccg
     1021 ttaatcttga aactgggaaa aaatttcaag cccatgggca agcattttta aacaaatgtt
     1081 atgacctaaa tgacgagatt tgtcttgaac atgacttaac aatcacggaa cgaggaaaga
     1141 aaaccgaaaa acgaaccatg tctgaaatta aattaaaaga aaaaaatgaa ccggtttgga
     1201 aagacgaaat tcggtttgcg attgatcaaa caatgaaaaa caaaaaaact cgaacgtata
     1261 cccaattttg tgattcactt aaaacattcg gcattcattg cttgaatcgc ggaaaaaatt
     1321 ttacttacga attggtccac aaaaagaaaa aagtacgatc caacaaacta ggaaaggatt
     1381 acgaaaagga gacgatttta catgagctgg acagacgaga acgaacctac aatcaacaac
     1441 gaaccaacga gctacgaaga gctcgagcta gcattcgcga aactgcagaa gaagtacgaa
     1501 gagaacaacg aacttttacg cctgaacaat cggaaattag accgactcga agaaaccatg
     1561 aacaacacgt tgaacgagga acagaccacc aacaagaatt atcgctctaa aacgttaaaa
     1621 gacatcgaaa acgaaataaa gagacaacgt gcctttcttg cgccttatac ccaagaaagg
     1681 atacaaacta cgatggattc acaagtcaaa aaactccacc aagcgctatt agaccagcga
     1741 aaccaagaac aaaaaaaggc tttaattcaa cgatcgatgg atattcttgc ccccttatgt
     1801 tgcttagtac taacattatt agccttttgg ttggtatggg gagaataaac agacatggca
     1861 ttctatttac cctatctact catttctgcc agtattttag gaattatttg gcttagctat
     1921 aaggcttatc aaacaagata ttcattaaaa ggctctaaaa ttcgatttaa gcgattttta
     1981 ttggtcgggg ttatatttgc cttatcaatc gttttaagtg gtttattcgg cgttttagag
     2041 ggtaataaac gtgtttcaag aagcatttta ttaggggata tcactcaaaa gtatgaagca
     2101 gcatgtgata aaaagaaaaa agagcaagca ttagcacaaa aaatggagga atttaccgct
     2161 tgttatgagg acatgaacaa tatttttgtg gatcaagaga aacgtttaac agataaaaac
     2221 atgaaaaaac tcactcgttt ttatcaaaac ttaccagaaa aacagcaaaa agaggtacaa
     2281 gaaaaatatg agcaagtaaa aaaagacgtg caatatttta aagatacgca gacagaagaa
     2341 gcttgttgta acttatttag cgatacaatc cctttttcaa ccagtgaaca agaacgaaaa
     2401 gaacggcaac aatcggtcac atacgaacgt tatgaaacac tgcttcagca agcaacaaac
     2461 atacaaaatc caacgaaaaa agaaacggca ttaaattatc tagaaagtgt caaggaatgg
     2521 ttggatcagc aacaacaaaa ttgaatttga ttagcaatca tgttaaactg aacctgcaaa
     2581 tgatcaaaca ttagatacac gaaagcccca gagaggtcgg attctctggg gctttttttc
     2641 aggttgatgt cgcctttatt gattttactt cttgtttcgg ttattcaacc aataactgaa
     2701 taaagagaca caaacttcta caagaagggg gcaaatgaac cgttcaaaca ttagatacac
     2761 ctccttccag tcgcaagact gtcggatagg cgacaagtta ttatacctta caaaataaaa
     2821 tctgtgtggt gtttttaagt atcccttatt ttcgggttgt tagggcttgc cctgaccgcc
     2881 tgtaaggcgc tttaagaaca agataaggtt ttttacccaa gactgtctta aaacgactta
     2941 gacgagctat cagaggcttc ccccacctcc gttactgact tgtttttagc aaaggagcct
     3001 ttaggcgact gcgcagtaca atgtgagagc atagcgatca cactttttta gcgaccgtag
     3061 ggagcaaggt tttgaattgg cagttttgaa aaatgcgcga acctgttggt agaaaaaaga
     3121 agtggttttt aggcgggaaa ttttgtcacc ctcttttata atctcttttt aaaccttttt
     3181 ttaaacctct ttaccgtcgg ctgtgtcctt actccttcaa tggatacagc gttttataaa
     3241 ctacattttg tctgctttta aactacattt tgtctgcttt taaactacat tttgtctgct
     3301 tttaaactac attttgtctg cttttaaact ctttttttgc actcacgtat gttataatat
     3361 gcattatcac agacaaaacg ttgtttataa cgtctgtatc cattgaagga gtaaggagga
     3421 atatctttgg caaatgaaat cgtgaaatac catcatgaat taaataccat tccgttgcgg
     3481 aaatttacac cagttgaaat gaatctattt ttctctattg tgtctagaat gcgcgaaaaa
     3541 ggcgataaaa cagtccgtct tacttttgac caactaaaag atttaagtaa ttataaagca
     3601 actgctaata atcgtttcat taatgattta gaaatgactt atgataaatt aatggattta
     3661 cgttttggta aaagaagtgc taatgggctc gatatagata gattcgttat gtttagtgag
     3721 tttaaaatac gtggaactgc taaagatcca tatgtagaca tacaagttta cgaaaaagct
     3781 ttacctttac taaacaattt agatgagtgg gttcgctatt cactccaaca atttaacgaa
     3841 ttacaaagta gctatgcaaa gactatgttt cgattactaa aacaatatcg aactaaaggt
     3901 tttgcttatt tttctaaaga ggattttcat gaactattag atattccaaa aagttactgg
     3961 aacaaccaag caaacgttga taatcgtgtt ataaagccta tacgacagga attaaccgca
     4021 atcttcaaag gattatcaat caaaaagaaa tacggaaaag ggcgaggaaa acctgtcgtt
     4081 ggctatcaat ttacgtttaa accagaaatc aaaaaagcag atgattttaa taaacatgca
     4141 ccaaccaaag aagaacaaaa taaagcattg gctttagctg ataaacaaaa agaatacgaa
     4201 gaacctgcta caacaattaa cgaaaaaatc aaacagaaga aacgagaact tgaactcctc
     4261 caacaaatca aacaactaga aaaagagaaa gaagctcttg aaaaacaaac agagtccgca
     4321 ctttcgaaaa aagagaaaaa aagtttatta tctgatttag ataatttatt ccagtagcaa
     4381 cctaggaggc aaccagtatg caaccacaag aaacctttac aataaaggaa ttgtcagaac
     4441 tttttaagat gagtaggcaa gcaatatcga aacatatcca aaaattagat tcttccatga
     4501 tagctaaaaa tgagcgtggt tataaggttg tattgcgttc tggagtattg caacttgctc
     4561 gcaacttagg caaccaaatg ttgctagata ggttgcacga gtacgaacaa gacaacgata
     4621 aaaatgttaa tataccaagc aaaaacgtgg ttacaaacaa gttgctagat cagttgcgtg
     4681 aagaaaatga atttttacga gaacaacttg ccctcagaga gcaaaataat tctcatatac
     4741 aaaaactact cgaccagcaa caacaactca ccctacaagc caacaaacaa atcgaaaaac
     4801 tgcaagaaaa actacagttg acttatacag aagaagctcc agagaatgac tcaacgactc
     4861 tttctgaagc aacagaaaca atagacaaac aaattgatac tgaaaaaaaa tggtggcaac
     4921 tttggaaata actggaggga acattcatga caagaaaaaa tcttctattc ggttctattt
     4981 tactaggaat tctaggattc agtccgatgt tattgtcagt gatcaataaa gggaataaac
     5041 atggtgaatt gaaattgaat ccaaattatt acaacagtcc taacgaagaa ttgtttgtaa
     5101 aagaaacaga taaataataa aaaagactgc tctttcgagc agccagttaa aaaataatga
     5161 gtgtttattc ttgaaaaagc aacgcgctta tgaagcgcta ccaaaaattt atcatatttt
     5221 attaaaaata acaacccagt aaaaaatgac tggtaaaaat tcacaaagaa acggaaagaa
     5281 gcaaccgcag aaaatcaagc ttaaaaagag cttttgacct agaccaatcg aaaatcacaa
     5341 agtgagcgcc tgtatctatt gctacaggcg ctacttggca tagtatacat tatgtcaaca
     5401 agaatttaat ataacatatt ttcctaatgc attctaacta taccacatta tcaaaaatat
     5461 aaattatctt tactaaataa aatattgaca agcctatacc ttttgtttta tgattttcac
     5521 aaagaaaacg ttttctatat atttttctgt atcaggagga aattctatga aaaaatttac
     5581 tttaggggta gctttgttaa ctattggggc agttgtttta ccgtcaacta ctatagttta
     5641 tgcttcagaa aattcccaag tggtatcatt aaacgaaaac aatgctactg aaaatgtagt
     5701 tattgagtac actgaagatg gtatggtcgt aactaaatat gaggatggtt atagtgcgga
     5761 agattttgca aattcttcag atatcatgac aagagctgca gttgcaagat ggggcaaaaa
     5821 ggaatataca aatattgctg taaccactgg agtagctgct aatgctatta atgctgcctt
     5881 ttgggcagga gggaaagcta gcacttctat gtttggtatc ccaggttggg caattggtgc
     5941 tttgttaaca ggtgcaagtt ggactaaaaa aggtgcttct ccagggaatg cagttgcaaa
     6001 agaatgggac aaaaatggta atggctggat tggattttat taccaaaggg gatatgatgc
     6061 agctggaaga gttgtagcta ctagatattc aacgttataa gttaaggagt gatgtttttg
     6121 atatctaaat cattaaaatc attaaaaaca tttactaaaa agaatccgca aatagctact
     6181 attcgtattt ttatatttgc tatagctgtt gctataattg ttaatatgct aagcggaggg
     6241 caattataca aatctggttt atttttagtg ttaatcttag ttgtcttaga cataataaag
     6301 tct
//
LOCUS       DACEEZ010000120         6201 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20313_2017
            SAMN12639966-rid9879223.denovo.123, whole genome shotgun sequence.
ACCESSION   DACEEZ010000120 DACEEZ010000000
VERSION     DACEEZ010000120.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639966
            Sequence Read Archive: SRR10023158
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6201)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6201)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741683.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741683.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:44:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,016
            CDSs (total)                      :: 2,962
            Genes (coding)                    :: 2,889
            CDSs (with protein)               :: 2,889
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 36 of 73
            Pseudo Genes (incomplete)         :: 29 of 73
            Pseudo Genes (internal stop)      :: 18 of 73
            Pseudo Genes (multiple problems)  :: 8 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6201
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639966-rid9879223.denovo.123"
                     /isolate="E20313_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(254..541)
                     /locus_tag="IWO89_002630"
     CDS             complement(254..541)
                     /locus_tag="IWO89_002630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002363589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin immunity protein"
                     /protein_id="HAQ0457327.1"
                     /translation="MDFTKEEKLLNAISKVYNEATIDDYPDLKEKLFLYSKEISEGKS
                     VGEVSMKLSSFLGRYILKHKFGLPKSLIELQEIVSKESQVYRGWASIGIWS"
     gene            complement(561..785)
                     /locus_tag="IWO89_002631"
     CDS             complement(561..785)
                     /locus_tag="IWO89_002631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002363584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin hiracin-JM79"
                     /protein_id="HAQ0457328.1"
                     /translation="MKKKVLKHCVILGILGTCLAGIGTGIKVDAATYYGNGLYCNKEK
                     CWVDWNQAKGEIGKIIVNGWVNHGPWAPRR"
     gene            complement(1425..1958)
                     /locus_tag="IWO89_002632"
     CDS             complement(1425..1958)
                     /locus_tag="IWO89_002632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF536 domain-containing protein"
                     /protein_id="HAQ0457329.1"
                     /translation="MQPQETFTIKELSDLFKMSRQAISKHIQKLDSSMIAKNERGYKV
                     VLRSGVLQLARNLDNQRLLDKLHKHEQNNDKNAYIPNKNVVANKLLDQLREENEFLRE
                     QLALKEQNNSHIQKLLDQQQQLTLQANQQIEKLQEQLQLTYTEESSENSSTTLSEATE
                     TMDEQTKNWKKWWQLWK"
     gene            complement(1979..2923)
                     /locus_tag="IWO89_002633"
     CDS             complement(1979..2923)
                     /locus_tag="IWO89_002633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RepB family plasmid replication initiator
                     protein"
                     /protein_id="HAQ0457330.1"
                     /translation="MANEIVKYHHELNTIPLRKFTPVEMNLFFSIVSRMREKGDKTVR
                     FTFDQLKDLSNYKATANVRFVEDLKATYEKILSLRFGRRSESGLSYDMFVMFTEFKIN
                     GDVENPYVDIRIYEKALPLLNNLDEWVRYSLQQFNELQSGYAKTMFRLLKQYRTKGFA
                     YFSKEDFLELLDIPKSYKQPDIDKRVIKPIRQELTAIFKGLTIKKKYGKGRGKPVIGY
                     QFTFKPEIKKADDFNKHAPTKEDQNKALALADKQKEYEEPATTINEKIKQKKRELELL
                     QQIKQLEKEKKALEKQTESALSEKEKKSLLSDLDNLFQ"
     gene            3560..3646
                     /locus_tag="IWO89_002634"
     CDS             3560..3646
                     /locus_tag="IWO89_002634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011302749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Fst family toxin"
                     /protein_id="HAQ0457331.1"
                     /translation="MFELFICPLLIEVCVSLFSYWLNNRNKK"
     gene            complement(3767..4456)
                     /locus_tag="IWO89_002635"
     CDS             complement(3767..4456)
                     /locus_tag="IWO89_002635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0457332.1"
                     /translation="MAFYLPYLLIFASILGIIWLSYKAYQTRYSLKGSKIRFKRFLLP
                     GVIFTLSIVLSGLFGVLEGNKRISRSILLGDITQKYEAACDKKKKEQVLTQKMEEFTT
                     CYEEMNDIFVNQEKRLTDKNMEKLTRLYQNLPEKQQKEVQDNYERTKKDVQYFKDTQT
                     EEACYNLFSDTIPFSTSEQERKERQQSVTYERYKALLQQATNIQNPTKKETALNYLKS
                     VKEWLDQQQQN"
     gene            complement(4701..5615)
                     /locus_tag="IWO89_002636"
     CDS             complement(4701..5615)
                     /locus_tag="IWO89_002636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /protein_id="HAQ0457333.1"
                     /translation="MATVKVSRTTSCSRAINYAEPRATVKTGINCDIDYAKSEMKQIR
                     MLYGKDDRVQAHLLIQSFRPGEITAEKANQLGAEYAKKIAPDHQIAIYTHTDKDHIHN
                     HIVINSVNLETGNKFQAHGQAFLNKCYDLNDEICLEHDLTITERGKKPEKRTMSEIKL
                     KEKNEPVWKDEIRFAIDQTMKNKKTRTYNQFCDSLKTFGIHCLNRGKNFTYELIHKKK
                     KVRSNKLGKDYEKETILHELDRRERAYNQQRTNELRRARASIRETAKEVRREQRTLTT
                     EQPEIRPTRRNHDQHVERGADYQQELSF"
     gene            complement(5597..5977)
                     /locus_tag="IWO89_002637"
     CDS             complement(5597..5977)
                     /locus_tag="IWO89_002637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="HAQ0457334.1"
                     /translation="MSEQNQYLASNHSKKYTYRSEPKQISFRVSESEFAKLKQSAEAL
                     QMSVPAFVKAKAQNARLVTPKVAPDIAQAIARDLAKAGGNINQIAKWCNTHQHDVDPG
                     DAQRLSENLKIMQKELQKIWQQLK"
BASE COUNT         1648 a         1160 c         1044 g         2349 t
ORIGIN      
        1 cccccactct ccacaataca aaaaagacta gcaaatgcta gccacttttt gtaaagtttc
       61 gcttgtttca ccttcggtga aattgttgca aatcaataat taggtagagc tacatttttt
      121 tcgttgttca aagattctta tgccatttac tattaataaa tctataaata tgataatttt
      181 tcggctctat aatttatggg actgatgaat cagaattgat tcttcagtcc cattttcatt
      241 ttattccaaa gaattaactc caaataccaa tagaagccca tcctctatat acttgagatt
      301 ccttactaac aatttcttgt aattctatta aagatttagg taatccaaat ttatgtttta
      361 aaatatatct tccaagaaaa ctacttaatt tcatactaac ttcaccaaca ctttttccct
      421 cactgatttc tttagaataa agaaagagct tttcttttaa gtcaggatag tcatctatag
      481 ttgcttcatt gtatacttta ctaattgcat ttaaaagttt ttcttcttta gtaaaatcca
      541 ttataataac ctccctacca ctaccttcta ggtgcccatg gaccatgatt aacccaacca
      601 ttaacaataa tttttccaat ttctccttta gcttgattcc aatctaccca acatttttct
      661 ttgttacaat aaagaccatt tccatagtaa gtagctgcat caacttttat tcctgtaccg
      721 atgccagcta gacaagttcc taatattcct agaataacac aatgttttaa tactttcttt
      781 ttcatgattg tttttccttt tcgttttttt aaaattgctt gttatattaa ataatcttag
      841 taattttatt atataacatt ttattatata acaatgatta acgtcataaa aagtagtttt
      901 gaattcataa tcgtttaaat aaaattataa gatcagttat ttaaacagct agtagacata
      961 atgtgtatac taccaagtaa cgcctgtacc aagggatcca ggcgctgatt ttgtgaattt
     1021 ttattggtct aggttaaaag ctctttttaa ggcttggttt tctgcggttg tttctttcta
     1081 attctttgtg aatattgatc ggtcattttt taaatacttg ttatttttgt ttgtgtttgt
     1141 tatgcttttt acatatctca tattcattta ttattctttt ctaaggttaa taataggata
     1201 atctctttca gaaagactgc tcgtacgagc agtcttttct tatttatcaa gtttttctac
     1261 aaatacttcc tcattttgac tgttgtagta attaggattg agttttagtt cccctttttt
     1321 atttcctttg ttggccactg ataataacat tgggctaaag cttaatattc ctagtaggat
     1381 agaaccgaat agcaagtgtt tttttgtcat aagtatttcc ttcttcattt ccatagctgc
     1441 caccattttt tccaattctt tgtttgttcg tccattgttt ctgttgcttc agaaagagtc
     1501 gttgaactat tctctgaaga ctcttccgta taagtcaact gcagttgctc ctgaagtttt
     1561 tcgatttgtt ggttggcttg cagggtgagt tgttgttgct ggtcgagtag tttttgtata
     1621 tgagaattat tttgctcttt gagggcaagt tgttctcgta aaaattcatt ttcttcacgc
     1681 aactgatcta gcaacttgtt tgcaaccacg tttttgtttg gtatataagc atttttatcg
     1741 ttgttttgtt catgtttatg caacttatct agcaaccttt gattgtctaa gttgcgagca
     1801 agttgcaata ctccagaacg caatacaacc ttataaccac gctcattttt agctatcatg
     1861 gaagaatcta atttttggat atgtttcgat attgcttgcc tactcatctt aaaaagatct
     1921 gacaattcct ttattgtaaa ggtttcttgt ggttgcatac tggttgcctc ctaggttgct
     1981 actggaataa attatctaaa tcagataata aacttttttt ctctttttcc gaaagtgcgg
     2041 actctgtttg tttttcaaga gcttttttct ctttttctag ttgtttgatt tgttggagga
     2101 gttcaagttc tcgtttcttc tgtttgattt tttcgttaat tgttgtagcg ggttcttcgt
     2161 attctttttg tttatcagct aaagccaatg ctttattttg gtcttctttg gttggtgcat
     2221 gtttgttaaa gtcgtctgct tttttgatct caggtttgaa cgtaaattga tagccaataa
     2281 caggttttcc ccgtcctttt ccgtatttct ttttgattgt tagtcctttg aagattgcag
     2341 ttagttcttg acgtataggt tttataactc gtttatctat atcaggttgc ttataacttt
     2401 taggaatatc taataattca agaaagtctt ctttggagaa atatgcgaaa cctttggttc
     2461 tgtattgctt taacagacga aacatagtct ttgcatagcc actttgtagc tcgttgaatt
     2521 gttgtaaaga atagcgtacc cactcgtcta aattatttaa caaaggcagt gctttttcgt
     2581 aaattcgtat atctacgtaa ggattttcta cgtccccgtt gattttaaat tctgtaaaca
     2641 ttacaaacat atcgtaagaa agcccagact cacttctacg accaaagcgt aaagataaga
     2701 ttttttcata agttgctttt aaatcttcta caaatcgtac atttgcagtt gctttgtagt
     2761 tacttaaatc ttttagttgg tcaaacgtaa aacgaactgt cttatcccct ttttcacgca
     2821 ttctagacac gatggaaaaa aacaaattca tttcaaccgg tgtaaatttt cttaaaggaa
     2881 ttgtatttaa ttcatggtgg tattttacaa tctcattagc catataatca cctcaatcca
     2941 attatgaatg acatttttta aatgtcaaga cattcatatt tgacgttcaa acagacaaaa
     3001 tgtccttcat aaacagacaa aatgtccttc ataaacagac aaaatgtcct tcataaacag
     3061 acaaaatgtc cttcataaca ttctgtatcc attggtagag taaggacaca gcttacggta
     3121 aagaggttta aaaagaggtt taaaaagaga tataaaaaga ggggggctaa ttttcccgcc
     3181 taaaaaccac ttcttttttc tttaaacagg ttcgcgcatt tttcaaaact gccaatccaa
     3241 aaccttgctt cctacggtcg ccaaaaaggt gtgatcgcta cgctctcaca ttgtactgcg
     3301 cagtcgccta aaggctcctt tgctaaaaac atgttggtaa agaggccttt gagcggttct
     3361 gaatgcttat ctaagccgtt ttaagacagt cttggataaa aatccttatc tagctttaaa
     3421 gcgccttaca ggcggtcagg gcaagcccta acaaccctaa aataagcgat acttaaaaac
     3481 accacacaag tttaattttt taagatataa taacttgtcg cctatccgac agtcttgcga
     3541 ctggaaggag gtgtatctaa tgtttgaatt gttcatttgc ccccttctta tagaagtttg
     3601 tgtctcttta ttcagttatt ggttgaataa ccgaaacaag aagtaaaatc aataaaggcg
     3661 acatcaacct gaaaaaaagc cccagagaat ccgacctctc tggggctttc gtgtatctaa
     3721 tgtttgatca tttgcatgtt tagtttaaca tgattgccga tcaaattcaa ttttgctgtt
     3781 gctgatctaa ccattccttg acacttttta gataatttaa tgccgtttct tttttcgttg
     3841 gattttgtat gttcgttgct tgctgaagca gtgctttata acgttcgtag gtaactgatt
     3901 gttgccgttc ttttcgttcc tgttcactag ttgaaaaagg gattgtatca ctgaataaat
     3961 tataacaagc ttcttctgtc tgcgtatctt taaaatattg cacgtctttt ttggttcgct
     4021 catagttgtc ttgaacctct ttttgctgtt tttctggcaa gttttgataa agacgagtga
     4081 gtttttccat gtttttatcc gtcaaacgtt tttcttgatt cacaaaaata tcgttcattt
     4141 cctcataaca agtggtaaac tcttccattt tttgtgttaa cacttgctcc tttttctttt
     4201 tatcacatgc tgcttcatac ttttgagtga tatcccctaa taaaatactt cttgaaatac
     4261 gtttatttcc ctctaaaacg ccgaataaac cacttaaaac gattgataat gtaaatataa
     4321 cccctggcaa taaaaacctc ttaaatcgaa ttttagagcc ttttaatgaa tatcttgttt
     4381 gataagcctt atagctaagc caaataattc ctaaaatact ggcaaaaatg agtagatagg
     4441 gtaaatagaa tgccatttct atttattctc cccataccag ccaaaaggct aacagtgtca
     4501 tgattaagca acataagggg gcaagaatat ccattgatcg ctgcataatt acttttttct
     4561 gttctttatt tcgctggttt aatagcgcat ggtggaattt tttgacttgt gaatcaattg
     4621 tagtttgtat tctttctttg gtataaggtg caagaaaagc acgttgtttc tttatttcat
     4681 tttctatgtc ttgtaacgtt ttagaacgat aattcttgtt ggtagtctgc tcctctttca
     4741 acgtgttggt catggtttct tcgagtcggt ctaatttccg gttgttcagt cgtaagagtt
     4801 cgttgttctc ttcgtacttc ttttgcagtt tcgcgaatgc tagctcgagc tcttcgtagc
     4861 tcgttggttc gttgttgatt gtaggctcgt tctcgtctgt ccagctcatg taaaatcgtc
     4921 tccttttcgt aatcctttcc tagtttgttg gatcgtactt ttttcttttt gtggatcaat
     4981 tcgtaagtaa aatttttccc acgattcaag caatgaatgc cgaatgtttt aagagaatca
     5041 caaaattggt tatacgttcg agtttttttg tttttcattg tttgatcaat cgcaaaccga
     5101 atttcgtctt tccaaacagg ttcatttttt tcttttaatt tgatttcaga catggttcgt
     5161 ttttcgggtt tcttccctcg ttcagtgatt gttaagtcat gttcaagaca aatctcgtca
     5221 tttaggtcat aacatttgtt taaaaatgct tgaccatggg cttgaaattt gttcccagtt
     5281 tccagattga cggagttaat gacaatgtgg ttatgaatat ggtctttgtc tgtatgtgtg
     5341 taaatcgcga tttggtggtc aggggctatt tttttcgcat actctgcccc tagttgattg
     5401 gctttttcag cggtaatttc tcctggtcga aaagactgga tcaataagtg tgcttgtaca
     5461 cgatcgtctt ttccatacag catgcgaatt tgtttcattt cgcttttggc ataatcaatg
     5521 tcacagttta tccctgtttt gacggttgct cgtggttcgg cgtaattgat cgctcggcta
     5581 caagaagtgg ttcggcttac tttaactgtt gccatatctt ttgtagctcc ttttgcatga
     5641 tttttaggtt ttcggataag cgttgggcgt cgccaggatc aacgtcgtgt tggtgtgtgt
     5701 tgcaccactt ggcaatttga ttgatattcc cgccagcttt ggctaaatcg cgcgcgattg
     5761 cctgagcaat gtctggcgcc actttcggtg tcaccagacg cgcgttttgt gccttggctt
     5821 tcacgaaagc cggcacactc atttgcaaag cttcagctga ctgcttcagc tttgcaaatt
     5881 cggattcgct cactcgaaaa ctgatttgtt ttggctcgga tcgataggta tattttttag
     5941 aatgattgct agccaaatat tgattttgtt cgctcataag tcccaccctt tcagtacgtc
     6001 ctacggacgt atcgttcgca ataccattgt acctaaaaag gtacaataag taaagctcac
     6061 taaagctcac taaagggctt ggcaagctct atatttgcta gccaaatacc tacgtatttg
     6121 gtgcaaataa ccgcttgtca aaggggcgca agctccccct tgaatccccc ctcaaaaccc
     6181 acgctccaca atacaaaaaa g
//
LOCUS       DACEEZ010000121         6139 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20313_2017
            SAMN12639966-rid9879223.denovo.124, whole genome shotgun sequence.
ACCESSION   DACEEZ010000121 DACEEZ010000000
VERSION     DACEEZ010000121.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639966
            Sequence Read Archive: SRR10023158
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6139)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6139)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741683.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741683.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:44:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,016
            CDSs (total)                      :: 2,962
            Genes (coding)                    :: 2,889
            CDSs (with protein)               :: 2,889
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 36 of 73
            Pseudo Genes (incomplete)         :: 29 of 73
            Pseudo Genes (internal stop)      :: 18 of 73
            Pseudo Genes (multiple problems)  :: 8 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6139
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639966-rid9879223.denovo.124"
                     /isolate="E20313_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            80..607
                     /locus_tag="IWO89_002638"
     CDS             80..607
                     /locus_tag="IWO89_002638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase"
                     /protein_id="HAQ0457335.1"
                     /translation="MKIKERRPLKKKQTKEKTINRVLLGLIVFLLAVIVISSIFYLRS
                     SRPAVQAKKEAVEIAKKYANLEEADHFYWFTRKKTYFSLTGTNDKGKEIAVIIPKSGE
                     KVKVLDQTKGLTENEAKQTIAAAHPEIQIEKAALGMYDDQPVWEVVGKETNGKLNYYL
                     IGFESGDEVKTIKGI"
     gene            628..1818
                     /locus_tag="IWO89_002639"
     CDS             628..1818
                     /locus_tag="IWO89_002639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HAQ0457336.1"
                     /translation="MELSKRTKRLEPSVTLAAAAKAKALKAKGRDVLSLTVGEPDFAT
                     PENIQEAAIEAIRNGKASYYTPTAGIPELRQAIVDYLKKNYGLVYEPSQTVVTDGAKF
                     ALYTLFQTILDPQDEVIIPVPYWVSYGEQVKLAEGLPVFVKGEESNSWKVTVEQLEQA
                     RSEKTKAVIINSPSNPTGMIYSKNELQAIGEWAVKNDILIVADDIYGRLVYNGNEFTP
                     IATISEAIKNQTIIINGVSKTYAMTGWRIGYAVGNPVIINGMIAVASQSTSNPTAVSQ
                     YAAVEALKGEQDTVEEMRNAFEERLNTLYSLVAELPGVSLEKPQGAFYLFPNVKETLR
                     MCKYENVTQWVEDLLEETGVALVTGEGFGAPENVRMSYATDRATLEEAVRRIKQFIES
                     KSQN"
     gene            1858..3156
                     /gene="asnS"
                     /locus_tag="IWO89_002640"
     CDS             1858..3156
                     /gene="asnS"
                     /locus_tag="IWO89_002640"
                     /EC_number="6.1.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016180901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine--tRNA ligase"
                     /protein_id="HAQ0457337.1"
                     /translation="MEQIQIIDSKNHVGETVTIGAWVANKRSSGKIAFLQLRDGTAFF
                     QGVVVKSEVSEEVFQTAKNLHQETSVLVTGEIREDSRSKFGYEIGITNVEVVSESHDY
                     PITPKEHGTDFLMDHRHLWLRSSRQHAIMQIRNEIIRATYEFFNQHHFVKIDPPILTG
                     SAPEGTTDLFETNYFDQKAYLSQSGQLYMEAAAMAFGKVFSFGPTFRAEKSKTRRHLI
                     EFWMIEPEMAFTHQEESLEVQEQYVAFLVQNVLDHCEYALDVLGRDKEVLKTYTKLPF
                     PRITYDDAIELLKKNGFDDIEWGEDFGSPHETFIANSFEQPVFILNYPKAIKPFYMKP
                     HPTREDVVICADMIAPEGYGEIIGGSERATDYDFLLEQIRNHGLDEKEYSWYLDLRKY
                     GSVPHSGFGLGLERTVTWIAGIDHVREAIPFPRLLNRIYP"
     gene            4332..5006
                     /locus_tag="IWO89_002641"
     CDS             4332..5006
                     /locus_tag="IWO89_002641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HAQ0457338.1"
                     /translation="MKKRILFIGDSITDAGRDRAEATDLGKGYPLLIAGKLISQYPTM
                     KFECMNRGIGGDKIADLQVRWQEDCLALQPDYVSILIGINDTWHNTSDQAIFGTEKSA
                     QQFESVYRKLLNELQTAEICNIILMEPFVLPITDDRHGWRKDLDPKIQIVRKLAKEYG
                     ALLVPLDGIFNALGIAYGFSKYTREDGVHPTIGGHEIIAQSWIDAIAAVDFFSGDKNE
                     RKKSEK"
     gene            5003..5344
                     /locus_tag="IWO89_002642"
     CDS             5003..5344
                     /locus_tag="IWO89_002642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0457339.1"
                     /translation="MMEIRWAVKEDLPQINQLITDHPESFVKAKTAADLNDKNKLLLV
                     AVTKEKRIIGFAQADVQQSEERVDQRPSGKLIQHTLVTKENTEEIQKKFAEYLMLEAK
                     KRRIKSIFAQF"
     gene            5374..5733
                     /locus_tag="IWO89_002643"
     CDS             5374..5733
                     /locus_tag="IWO89_002643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YccF domain-containing protein"
                     /protein_id="HAQ0457340.1"
                     /translation="MSCLGNVIWFIFGGLIGGLSWLAAGILWCITIIGIPIGMQCFKI
                     ASLSFAPFGKEVIYHQSGMSLIVNIIWLIVSGLPIALAHVVSGILLCITIIGIPFAKQ
                     SFKLARLALMPFGAEIV"
     gene            5937..6116
                     /locus_tag="IWO89_002644"
                     /pseudo
     CDS             5937..6116
                     /locus_tag="IWO89_002644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308695.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cyclase family protein"
BASE COUNT         2090 a         1063 c         1341 g         1645 t
ORIGIN      
        1 gaattcttaa agaaaaatga agaaggaagt tagaggagct gtcttttttc ataattaaaa
       61 tctgctataa tgaaaaggaa tgaagataaa ggagcgacga cccttgaaga aaaaacagac
      121 aaaggaaaaa acgattaatc gcgtgttact tggcttgatc gtatttttat tagcggtcat
      181 cgtgatcagc tcgatttttt acttgcgttc cagtcgtccg gcggtgcagg cgaagaaaga
      241 agcagtagaa atcgcaaaga aatatgcaaa tcttgaggaa gccgatcatt tttattggtt
      301 tactcgaaag aagacatact tttcactgac aggtacaaat gataaaggaa aagaaatcgc
      361 cgttatcatt cctaaatctg gtgaaaaagt aaaagtattg gatcaaacaa agggactgac
      421 tgaaaacgaa gcaaaacaga caattgctgc ggcccatcct gaaatccaaa tcgaaaaagc
      481 tgctttaggg atgtatgatg atcagcctgt gtgggaagta gtaggaaaag aaacaaatgg
      541 gaaactgaat tattacctga ttggctttga atctggagat gaagtcaaga caatcaaggg
      601 aatttaaaag gtaaaggaat gaatgggatg gagttatcaa aaagaacaaa acgattagaa
      661 ccgtctgtca ctttggcagc tgcggcaaag gccaaggcat taaaagcaaa aggcagagat
      721 gtgctgagtt tgactgtggg agaaccggat tttgcgacac cagaaaacat ccaagaagca
      781 gcaatcgaag caatccgcaa cgggaaagct agctattata caccaacagc aggaatccca
      841 gagcttcgac aggcaatcgt tgattatctg aagaaaaact atggacttgt ctatgaacct
      901 tcacaaacag tcgtaacaga cggagcgaaa tttgctttgt ataccctttt ccaaacgatc
      961 cttgacccgc aagatgaagt catcattcct gtgccttact gggtcagtta cggtgagcaa
     1021 gtaaaactgg cagaaggact tcctgtcttt gtgaaagggg aagaatccaa tagctggaaa
     1081 gttactgttg aacagctaga acaagcacgt tcagaaaaaa caaaagccgt aatcatcaat
     1141 tctccatcga atccgactgg aatgatttat agcaagaatg aactgcaggc aatcggtgaa
     1201 tgggcagtaa aaaatgatat tttgatagtt gctgatgata tttatggtcg tttagtttat
     1261 aatggcaatg agtttactcc aattgcaacg atttcagaag caatcaaaaa tcagactatc
     1321 atcatcaacg gcgtgtctaa aacatatgcg atgacaggct ggcgcattgg ttatgcggtg
     1381 ggaaatcctg tgatcatcaa tggaatgatc gctgttgctt cccagtcaac cagtaatccg
     1441 acagcagtca gtcaatatgc agctgttgaa gcattgaaag gcgagcaaga tacagtggaa
     1501 gaaatgcgga acgcctttga agaacgcctg aatacgttat attcgcttgt ggcagaactg
     1561 ccgggtgttt ctcttgaaaa gccacaagga gctttttatc tttttcctaa tgtaaaagaa
     1621 acgctacgca tgtgtaaata tgaaaatgtc acacaatggg tagaagatct tttagaagaa
     1681 acaggggtag cgcttgtgac tggcgaaggc tttggggcac cagaaaatgt ccgcatgagt
     1741 tatgctactg atcgcgctac gttagaagaa gcggtacgca gaatcaagca atttattgag
     1801 agtaaaagtc aaaattaatc ctaaagaaat tcaaggaata taaaaggaga gtcatacgtg
     1861 gaacaaattc aaatcatcga ttcgaaaaac catgtgggag aaacagtaac aatcggcgct
     1921 tgggtcgcaa ataagcgatc aagcggaaaa atcgcatttt tacaattaag agacggaaca
     1981 gcctttttcc aaggtgtagt tgtaaaaagc gaagtgtcgg aagaagtttt ccaaacagct
     2041 aaaaatctac accaagaaac atccgtgtta gtaactggag aaatccgtga agacagtcgt
     2101 tcaaaatttg gttatgaaat cggaattaca aatgtggaag tggtaagcga gagccacgat
     2161 tatccgatca caccaaaaga acacggaaca gacttcttga tggatcatcg tcatttatgg
     2221 ctgcgttctt caagacaaca tgcgattatg cagatcagaa atgaaatcat tcgtgcaacg
     2281 tatgaattct ttaatcaaca tcattttgtc aaaattgatc cgccgatctt gactggttct
     2341 gcaccagaag gcactacgga tctattcgaa acaaattact ttgatcaaaa agcttattta
     2401 tctcaaagtg gccaattata catggaagct gctgctatgg ctttcggaaa agtcttttca
     2461 ttcggtccga ctttccgtgc tgaaaaatca aaaacacgac gtcatttgat cgaattctgg
     2521 atgatcgaac cagaaatggc tttcactcat caagaagaaa gtttagaagt acaggaacaa
     2581 tatgtagcat tcttagtaca aaatgtttta gatcattgcg aatatgcatt agatgtacta
     2641 ggacgagaca aagaagtctt aaaaacttat acaaaattgc cattccctcg tattacttac
     2701 gatgatgcaa tcgaattact gaagaaaaat ggatttgatg acatcgaatg gggcgaagac
     2761 tttggttcac ctcatgaaac gttcattgcg aattcattcg aacagcctgt ctttatccta
     2821 aactatccaa aagccatcaa gccattctac atgaaaccac atccaacaag agaagacgtg
     2881 gtaatttgtg cagatatgat cgcaccagaa ggttacggtg aaattattgg cggaagcgaa
     2941 cgggcaacag actatgattt cttgctagaa caaatccgta atcacggttt ggatgaaaaa
     3001 gaatattcat ggtatcttga tctacgtaaa tacggttctg ttccgcactc tggttttggt
     3061 ctaggccttg aacgaacagt cacttggatt gctggtatcg accatgtacg tgaagctatt
     3121 ccattcccac gtttgttgaa ccgaatttat ccataagagg tgatgaaaaa gctgttctgc
     3181 atcaagtaat tagaacagcc aagcaaaaac agctgttcat gtttttgaat tggcgggact
     3241 tattaccgca gatgtaagct tttgaatacc gtttagtgga gaggttgtga aaaagctgtt
     3301 tagtttcgag tattaagaaa aattttgtgg aaatggcttt ccacattttt gcataaattt
     3361 ggcttaatac cgagaaactg gcttttgaac accgtttaag gaagaggttg tgaaatcagt
     3421 gccttgacct gagtattaga gaaacaaaac ggaaaaatag cttctcatat tttaccgttt
     3481 ttttagctaa tatcgagggt cagctgattg aacaccgttt atatcaagag gttgtgcgcc
     3541 tgccgttcag ctccgagtca caaggagtaa tttcaaaaaa cagtttttca tgtttgatga
     3601 ctattgtgac ttgtgaccga ggagttggcc ggagagcaac gtttatttaa aaaaaagaac
     3661 tgcgaactct cgcagttctt tttttaaaat acgggataaa ggaagtttcc agcagacaag
     3721 agaagaaagc aaaagaaatt gctatttgat tcacaaggtt ctataacgaa atcgcaatca
     3781 atcaaacata tacaaagaag gttattcaaa tggaaatctg atttgatgat gcttctgctg
     3841 ttgaaataaa gcagcatcta gtacctgaaa acaaactaat agtaacattc gaagatggag
     3901 taggtccgta ttttcagcat gcaatgatcc atatgtaagt ttaattcatc cttgaaatca
     3961 tcggaccaga taaagttatg accaatctat cccaagcaat ctagaagaga tattagtaaa
     4021 agaacattta ctggaagtct tagatgagcc tataaatata tgttctgatg gcgtcatcag
     4081 tggtctgatt gacggaagct taggtgtgat cgatttctct ggaccaaaca acgaaggaat
     4141 caaggggaat ccggcacgtt aagaaaaaaa gtataaaaaa ctagacactt gatgagcgtt
     4201 cattcaattg attgaaggga ctaggctggt gtcatttttt atacatatat tttctctttc
     4261 gtcttgatag ggcttatggg ataatataag gaagaaaatc atcgttttat tcaaaaagga
     4321 gtttttgaca aatgaaaaaa cgtatattat ttatcggtga cagcattaca gacgcaggaa
     4381 gagaccgagc agaagcaact gatctaggta aaggttatcc acttttgatt gccggaaaac
     4441 taatctccca atatccaacg atgaaatttg aatgcatgaa ccgtgggata ggaggagaca
     4501 aaattgcaga tctgcaagta cgctggcaag aagattgttt agctttacaa cctgactatg
     4561 tatcgatttt gatcggaatc aatgatacct ggcataatac tagcgatcag gctatattcg
     4621 gaactgagaa atctgcgcag caatttgaat ccgtttatcg aaaattacta aatgaacttc
     4681 aaacagcgga aatctgcaac atcatcttga tggagccgtt tgtacttcct ataaccgacg
     4741 accgtcatgg ctggcgtaaa gacttagacc ctaaaatcca aattgttcgt aaacttgcta
     4801 aggaatatgg cgcattgctg gttcctttag atggcatatt caatgcttta gggattgctt
     4861 atggtttttc gaaatataca agagaagatg gggtccaccc aacgattggc ggacatgaga
     4921 tcattgcaca aagctggata gatgcaattg ccgcagtcga tttttttagt ggagataaaa
     4981 atgagcgaaa gaaaagtgag aaatgatgga aatacggtgg gcagtaaaag aagatttacc
     5041 tcaaatcaat cagctgatta ctgatcatcc agaatcattc gtaaaagcaa aaacggcagc
     5101 tgacctaaat gataagaata aactattgct cgttgctgta acaaaagaaa aacggattat
     5161 cggatttgcg caagcggacg tgcaacagtc agaagaacgg gtggatcagc gaccatcagg
     5221 aaaactaatc caacacacat tagtaacaaa agaaaatacg gaagaaatcc aaaaaaaatt
     5281 tgcagaatac ttgatgctgg aagctaaaaa aagaagaata aaatcaattt ttgcccagtt
     5341 ttaatcaaaa acagtaatag aaggagaaat acaatgagtt gtttaggaaa tgtcatctgg
     5401 tttatattcg gcggactaat cggtggtctt agctggttag cagcggggat actttggtgt
     5461 atcacgatca taggtatccc aatcggtatg caatgtttta agatcgcctc cctttctttt
     5521 gcgccttttg gcaaagaagt aatttatcac cagtctggca tgtcattgat cgttaatatc
     5581 atctggctga tcgtttcagg cctaccaatt gctctcgcac atgtcgtcag cggcatcctt
     5641 ctatgtatca cgatcatagg gatacctttt gccaagcaat ctttcaaact tgctcgattg
     5701 gcgttgatgc cattcggtgc agaaattgta taaaaagaga ataataaagg ttagaaaacg
     5761 catagagtca ggtgggatac atgtatttca cctgactcta tgtgttttcc tgtataagtt
     5821 ctcccatgtg acttttacgt cttttattta tatgggatta ctgtttatgg aaagttgaaa
     5881 taattttcat taaagaactc tttctatatt tttgaaagcg tagtggagct tcgaatatct
     5941 gggaaggaca ttttgcaggg attgaaaaaa gatattgtcc tatcgaaaaa ttaatgaatt
     6001 tagacaaatt gccagcaaca ggcttcaaac ttagttgcta tcctgtcaag atcaagaagg
     6061 caagtgcggg ttggataaga gcaggggcaa tgatcgaaga gaaaaagaaa gaataagcca
     6121 aaacatctgt atggaagaa
//
LOCUS       DACEEZ010000122         6133 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20313_2017
            SAMN12639966-rid9879223.denovo.125, whole genome shotgun sequence.
ACCESSION   DACEEZ010000122 DACEEZ010000000
VERSION     DACEEZ010000122.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639966
            Sequence Read Archive: SRR10023158
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6133)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6133)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741683.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741683.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:44:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,016
            CDSs (total)                      :: 2,962
            Genes (coding)                    :: 2,889
            CDSs (with protein)               :: 2,889
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 36 of 73
            Pseudo Genes (incomplete)         :: 29 of 73
            Pseudo Genes (internal stop)      :: 18 of 73
            Pseudo Genes (multiple problems)  :: 8 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6133
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639966-rid9879223.denovo.125"
                     /isolate="E20313_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(207..1169)
                     /locus_tag="IWO89_002645"
     CDS             complement(207..1169)
                     /locus_tag="IWO89_002645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018375965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family transcriptional regulator"
                     /protein_id="HAQ0457341.1"
                     /translation="MVAKLTDVAELAGVSPTTVSRVINNKGYLSEKTKQKVHEAMKTL
                     GYKPNNLARGLQGKSPQLIGLIFPNISNIFYSELIEYLEIELFKHGYKTIICNSQNDP
                     AKEREYIEMLEANQVDGIISSSHNLGIADYERVRAPIVAFDRNLAPNVSIVSSDNFEG
                     GKLAAKTLQKNGCQNTIMITGNDNTDSPTELRALGFSFQNPNGKIFKIPNNLSTIRRE
                     MEIKSTIINHKPDGIFVSDDLTAILTMKIAHQLKLNIPEDLKIIGYDGTSFIEKFFPQ
                     LTTIRQPIDEIASLIVDILLKKIKGEKTNKDYILPISILSGGSI"
     gene            complement(1171..2610)
                     /locus_tag="IWO89_002646"
     CDS             complement(1171..2610)
                     /locus_tag="IWO89_002646"
                     /EC_number="3.2.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sucrose-6-phosphate hydrolase"
                     /protein_id="HAQ0457342.1"
                     /translation="MNLPREIRYRAYSDWTKDEMDKIKDNVKRSPWRASYHIEPKTGL
                     LNDPNGFSFFNGKYTLFYQNWPFGAAHGLKEWVHNESDDLVHFHVTGAELLPDTKRDS
                     HGAYSGSAYEIEGNLFLLYTGNVRNENWERDTLQIGAWMDKDYNITKGENVLIHKPAD
                     VTDHFRDPQMFNYEGQLYAIVGAQNLEKSGFIKLYKADDNNVETWEEVGNLDFGGTGS
                     EYMIECPNLVFVDKKPVLIYCPQGLDKSELNYGNIYPNTFKICQSLDIEKVKLVGASE
                     IQNLDYGFEAYATQGFNTPDGRALIVSWIGLPDIDYPTDKYDYQGAMSLVKELSIKDG
                     KLYQYPVEAITSLRASSEQFTAKAETNNTYELELTFPPNQKSELLLFSDSKGNGLSLI
                     VDTKEGKIILDRSKAGIQYATDFGTIRECSIDTKETTANIFVDNSIIEIFINKGEKVF
                     TSRVFPENHQNGIQIKSGEPTGKYFELKY"
     gene            2795..4741
                     /locus_tag="IWO89_002647"
     CDS             2795..4741
                     /locus_tag="IWO89_002647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS beta-glucoside transporter subunit IIBCA"
                     /protein_id="HAQ0457343.1"
                     /translation="MNYSKVASDVIQAVGKDNLIAAAHCATRLRLVLKDDTKVNQKAL
                     DENPDVKSTFKINGQYQVIIGAGDVNFVYDELIKKTGLSEISTDDLKQIASKDKKFNP
                     IMALIKLLSDIFVPIIPALVAGGLLMALQNFLTAAGLFGPKSIEEMYPAIEGISSMIQ
                     LMSAAPFMFLPILVGISAAKRFGANQFLGAAIGMIMTTPDLGGSTEYWNIFGYHVSQT
                     NYAYQVIPVLAAVWILSILEKFFHKKLPSSIDFTFTPLLSVMITGFLTFTVIGPVMLL
                     LSNGITDIIVWLYNTTGFIGMGIFGGTYSLIVMTGLHQSFPAIETQLLSAWTNGIGHG
                     DFIFVVASMANVAQGAATFAIWFLTKNSKTKSLASSAGVSALLGITEPALFGVNLKYR
                     FPFFCALIGSGIATAVAGLLKVVAVSLGSAGFLGFLSINATSIPFYVLCELISFVVAF
                     AITYFYGKTKAAGIFKAEEIAEQTPVNSSKVATTDPISTDVESTQTKVSSETIVSPIS
                     GKVISLSSVNDPVFSSEAMGKGIAIKPNGNTVHSPVDGTIQLTFETGHAYGIKSNNGA
                     EVLIHIGIDTVSMGGKGFTQKVTTNQKVRKGDILGAFDRSMIEKEGLDDTTMIIVTNT
                     SDYSEVNIVAENTVTEGTDLLEIK"
     gene            5005..5877
                     /locus_tag="IWO89_002648"
     CDS             5005..5877
                     /locus_tag="IWO89_002648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HAQ0457344.1"
                     /translation="MLYGAIEAGGTKFVCAIGDEEMTIKERVSFPTTTPEETMPLVID
                     FFKQYQADLAGIGIGSFGPIDIHRDSATYGYITSTPKLAWQNFDFIGTMKKEFPIPIS
                     WTTDVNAAAYGEYVFGSGKGLSSVVYYTIGTGVGGGALQEGRFIEGFSHPEMGHMLVV
                     PHPKDDFAGSCPFHGNCLEGMAAGPAIEKRLGKKGQEVAEDDPYWEIEASYIAQCAYN
                     TTLMFSPDVIIFGGGVMKQRHMLQNVHDAFAKLVNGYVETPELSKYIVTPALEDNAGT
                     LGCLALAREAVLHS"
BASE COUNT         1954 a         1056 c         1080 g         2043 t
ORIGIN      
        1 cgtccaattt cgtaaaacaa ccgtaacagt tgtgtaaaaa tatcatttta gagcatgaaa
       61 aaaggagtgg atttctccac tccaaaaaag tctaactttt gggggtcact acaagttgac
      121 aactcctttt tgtttacaaa tcaattggat atctacaaat tgtattttct aacgaatttt
      181 atagacatta aaaaattaat ttaacgttaa atacttcctc cagaaagtat tgagattggt
      241 aaaatataat ccttatttgt cttttcgcct ttaatttttt tcagaagaat atcaacaata
      301 agacttgcaa tttcatcaat tggttgtctt attgttgtca attgtgggaa aaatttctca
      361 ataaaagagg taccatcgta tccaataatt tttaaatctt ctgggatatt aagtttaagt
      421 tgatgagcaa ttttcatagt gagaatggct gttaaatcat cagaaacaaa aataccatct
      481 ggtttgtgat ttataatagt tgactttatt tccatttcac gacggatagt agacagatta
      541 ttaggaattt tgaaaatttt tccatttgga ttttgaaaag aaaaacctag tgcacgtaat
      601 tctgtgggag aatccgtgtt atcatttcct gtaatcataa tggtattttg acaaccattt
      661 ttttgtaaag tcttagctgc aagttttccc ccttcaaagt tatctgaaga aacaatggaa
      721 acattaggag ctaaatttct atcaaaagca acaataggtg ctctcacacg ctcataatca
      781 gcaattccaa gattatggct tgatgagatt atgccatcaa cttggttggc ttcaagcatt
      841 tcaatgtatt ctctctcttt agcaggatca ttttgactat tgcaaataat cgttttatat
      901 ccatgtttaa ataactctat ttcgagatac tcaatgagtt cagagtaaaa gatattacta
      961 atattaggaa aaataagacc aataagctga ggtgactttc cttgaagtcc acgtgctaaa
     1021 ttatttggtt tataaccaag agttttcata gcttcatgaa ctttttgttt tgttttttct
     1081 gataaatatc ccttattatt aatgacgcgt gaaacggttg ttgggctaac tccagctaat
     1141 tcagcgacat cagttaattt tgctaccatt ttaatatttt aattcaaagt attttccagt
     1201 aggttcgcct gatttgattt gaataccatt ttggtgattt tcagggaata cacgactagt
     1261 aaacactttt tctcctttat taataaaaat ctcaatgatt gagttatcta caaaaatgtt
     1321 agcagttgtt tctttagtat caattgagca ttcacgaatc gtaccaaagt cagtggcata
     1381 ttgaatgcct gctttgctgc gatctaaaat aatttttcct tctttggtat ctacaatcaa
     1441 gctaagtcca tttcctttac tatcagagaa aagaagtaac tccgatttct gatttggagg
     1501 aaaagttagt tcaagctcat aagtattatt tgtttctgct tttgctgtaa attgttcaga
     1561 actagcacgt aatgatgtaa tagcttctac tggatattgg taaagtttac catcttttat
     1621 tgaaagttct tttacaagac tcattgctcc ctgataatca tacttatcag ttggataatc
     1681 gatatcaggt agtccaatcc aactgacaat cagagcacga ccatcaggag tattgaatcc
     1741 ttgagtcgca tacgcttcaa atccataatc aagattttga atttctgaag cgcccacaag
     1801 ttttactttt tctatgtcaa gcgattgaca gatcttaaaa gtattcggat agatattccc
     1861 ataatttagc tcagatttat ccaacccttg aggacaataa atgagaacag gttttttgtc
     1921 tacaaatact agatttggac actcaatcat gtattctgaa ccagtgccgc caaaatcaag
     1981 gttgccaact tcctcccatg tttccacgtt attgtcatcc gctttgtaaa gtttgataaa
     2041 gcctgatttt tctaaatttt gtgctccaac aatcgcatac aattgtcctt cataattaaa
     2101 catttgtgga tcacgaaaat gatcagttac atcagcaggt ttatgaataa gaacattctc
     2161 acctttcgta atgttgtagt ctttatccat ccatgctccg atttgaagtg tatcacgttc
     2221 ccaattttca tttcgaacgt ttcctgtata gagtaagaat agattcccct ctatttcata
     2281 tgcagaaccg gaataagcgc cgtggctatc tcgttttgtg tctggaagaa gttctgctcc
     2341 tgttacatga aaatgaacta aatcatctga ttcattgtgt acccattctt tcaacccatg
     2401 agcagctcca aatggccaat tttgataaaa aagcgtatat tttccgttaa aaaaagaaaa
     2461 accattagga tcgtttaaca atcctgtttt aggttcgata tgatagctag cacgccaagg
     2521 tgatcgtttt acattatcct taattttatc catctcatct ttagtccaat cagaataagc
     2581 acgatatcgt atttctcgag gtaagttcat attttactcc ttcaatttat ctttttacaa
     2641 ctagtatggt aaacgttttc cgttgaaaaa tcaatcattt catttaaaaa aataaactat
     2701 atgacaaacg tttgacatat ttttaagata tgctaaaata aatttgcatt tcagtgaacc
     2761 gttttcacgt aaaaataaaa aaggagtgat tcgaatgaat tacagtaaag tagcttccga
     2821 cgtaatacaa gcggtcggaa aagataacct tattgcagct gcacactgcg ctacacgtct
     2881 tcgtctcgta ctaaaagatg acacgaaagt gaatcaaaaa gctttagacg aaaatccaga
     2941 tgtgaaaagc acatttaaaa ttaatggaca atatcaagtg attatcggtg caggtgatgt
     3001 taatttcgtt tatgacgaac ttatcaaaaa aactggactt tcggaaattt caacagatga
     3061 tttaaaacaa attgctagta aagataaaaa attcaacccc attatggcat taattaaatt
     3121 attatcagat atttttgttc caattatacc tgcccttgtt gcaggtggtt tattaatggc
     3181 attacaaaac tttttaacag ctgctggact ttttggacca aaatcaattg aagaaatgta
     3241 tcctgcgatt gaaggtattt cctcaatgat ccaactgatg tccgctgcac cattcatgtt
     3301 cttaccaatt ttagtaggta tttcagctgc taaacgtttt ggagcaaacc aatttcttgg
     3361 tgctgcaatt ggtatgatca tgacaacacc agatcttggt ggctcaactg aatattggaa
     3421 tatttttggt tatcatgttt cacaaacaaa ctatgcttat caagttattc cagttttagc
     3481 agctgtttgg atattatcta tattagaaaa attctttcat aaaaaattgc catcatctat
     3541 tgactttaca ttcacgccat tattatcagt aatgattact ggatttttga ctttcacagt
     3601 tattggccct gtaatgttat tgttgtctaa tggaattaca gacattattg tttggctata
     3661 taacactact ggatttattg gaatgggtat ttttggagga acatattcac ttatagtcat
     3721 gactggttta catcaatcat ttccagctat tgaaactcaa ttactttcag cgtggacaaa
     3781 tggtatcgga catggagact ttatctttgt agttgcatct atggcaaacg ttgctcaagg
     3841 tgctgcaaca tttgccattt ggttcttaac taaaaattct aagacaaaaa gtcttgcttc
     3901 ctctgcgggt gtctcagcac ttcttggaat tacagagcct gccttgttcg gagttaactt
     3961 aaaataccgc tttccattct tttgtgcttt aattggttca ggaattgcta cagcagtagc
     4021 aggattactt aaagtagttg ctgtttcact tggttcagca ggtttcttgg gatttctttc
     4081 aattaatgca acatctattc cattttacgt attgtgtgaa ttgattagct ttgtagttgc
     4141 ttttgcaatt acttacttct acggtaaaac aaaagctgct ggtatcttta aagcagaaga
     4201 aattgctgaa caaacaccag ttaattcttc aaaagtagca acaacagatc caatatctac
     4261 tgatgttgaa tcaacacaaa caaaagtttc atcagaaaca attgttagtc ccatttctgg
     4321 taaagtgatt agtctttctt cagtaaatga tccagtcttt tcatcagaag ctatgggaaa
     4381 agggattgcc attaaaccaa atggaaacac ggtccattct ccagtagatg gtactattca
     4441 actcacgttt gaaactggac atgcttatgg tattaaatca aataatggtg ctgaagttct
     4501 aattcatatt ggtattgata ctgtatcaat gggaggaaaa ggatttacac aaaaagttac
     4561 tactaatcaa aaagttagaa aaggcgatat tcttggagca tttgaccgtt caatgattga
     4621 gaaagaaggt cttgatgata caacaatgat tattgtgacc aatacttcgg attattcaga
     4681 agtaaatata gtagctgaaa atacagtcac tgagggtact gatttacttg aaatcaaata
     4741 aatatatttc cctgagtaaa gatggaattt tctttccaaa atcaaataat atagattaat
     4801 caaatatttg agaataacat tttatttaat cttttcatat atcaattgta tgaaaaatta
     4861 ttagaatttt aaaaaatgat aaaaattcaa aaagaaaaaa tagtagttat ttacttgtca
     4921 tgttactaaa taaaaaaata atttaaatat gatatatagt catttattta gtgatttcat
     4981 tctaaaataa agggagaaat gtctatgcta tacggtgcaa ttgaagcagg cggcacaaaa
     5041 tttgtgtgtg ccattggcga tgaagaaatg acgatcaagg aaagagtgag ctttccgaca
     5101 acgacacctg aggaaacaat gcctttagtc attgacttct tcaaacaata ccaagcagat
     5161 ttagcaggga ttgggattgg ctcatttggg cctatcgata tccatcgcga ttccgccact
     5221 tatgggtaca ttacctcgac cccgaaattg gcatggcaaa attttgattt catcggtacc
     5281 atgaaaaaag aatttccgat cccgatttct tggacaacag atgtcaatgc ggctgcctat
     5341 ggtgagtatg tcttcggcag tggcaaagga ttgagcagtg tcgtttacta tacgatcggt
     5401 acgggtgttg gcggcggtgc cttacaagaa gggcgcttta tcgaagggtt tagtcatcca
     5461 gaaatggggc atatgctcgt cgtccctcat ccaaaagatg actttgctgg cagctgtcca
     5521 tttcatggca actgtttaga agggatggcc gctggacccg ccatcgaaaa acgcttaggg
     5581 aaaaaaggac aagaggttgc agaagatgat ccttattggg aaatcgaagc ctcctatatt
     5641 gctcaatgtg cctacaatac aaccttgatg ttttctccag atgtgatcat ttttggcggt
     5701 ggtgtgatga aacaacgcca tatgctgcaa aacgtccacg acgcatttgc aaaattggtc
     5761 aatgggtatg tcgaaacacc tgagttaagc aaatatatcg tgaccccagc attagaagac
     5821 aacgctggaa cgctcggttg tttggcattg gctcgagaag cggtcttaca ttcataaacg
     5881 aaaaaaggct cttcgacatt tagtgttggt agagaaagat acaactgaaa atttagtcat
     5941 aaccagttag gcagagtgct taactggttt ttgttttact tggaagatca agccttgaat
     6001 gagataaatt cttgctcgaa agttatggaa atttcgatag ccgtaggcca cttgtattgt
     6061 gcaatagttt tttacacgcc gtttgtataa ttaagcgaga caaataaaaa agccatttat
     6121 gttacactca aat
//
LOCUS       DACEEZ010000123         6131 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20313_2017
            SAMN12639966-rid9879223.denovo.126, whole genome shotgun sequence.
ACCESSION   DACEEZ010000123 DACEEZ010000000
VERSION     DACEEZ010000123.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639966
            Sequence Read Archive: SRR10023158
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6131)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6131)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741683.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741683.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:44:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,016
            CDSs (total)                      :: 2,962
            Genes (coding)                    :: 2,889
            CDSs (with protein)               :: 2,889
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 36 of 73
            Pseudo Genes (incomplete)         :: 29 of 73
            Pseudo Genes (internal stop)      :: 18 of 73
            Pseudo Genes (multiple problems)  :: 8 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6131
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639966-rid9879223.denovo.126"
                     /isolate="E20313_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            96..2537
                     /locus_tag="IWO89_002649"
     CDS             96..2537
                     /locus_tag="IWO89_002649"
                     /EC_number="1.1.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA reductase,
                     degradative"
                     /protein_id="HAQ0457345.1"
                     /translation="MKEVVMIDAARTPIGKYRGSLSPFTAVELGTLVTKGLLDKTKLK
                     KDKIDQVIFGNVLQAGNGQNVARQIALNSGLPVDVPAMTINEVCGSGMKAVILARQLI
                     QLGEAELVIAGGTESMSQAPMLKQYQSETNEYGEPISSMVNDGLTDAFSNAHMGLTAE
                     NVATQFSVSREEQDRYALSSQLKAAHAVEAGVFSEEIIPVKISDEDVLSEDEAVRGNS
                     TLEKLGTLRTVFSEEGTVTAGNASPLNDGASVVILASKEYAENNNLPYLATIKEVAEV
                     GIDPSIMGIAPIKAIQKLTDRSGMNLSTIDLFEINEAFAASSIVVSQELQLDEEKVNI
                     YGGAIALGHPIGASGARILTTLAYGLLREQKRYGIASLCIGGGLGLAVLLEANMEQTH
                     KDVQKKKFYQLTPSERRSQLIEKNVLTQETALIFQEQTLSEELSDHMIENQVSEVEIP
                     MGIAQNFQINGKKKWIPMATEEPSVIAAASNGAKICGNICAETPQRLMRGQIVLSGKS
                     EYQAVINAVNHRKEELILCANESYPSIVKRGGGVQDISTREFMGSFHAYLSIDFLVDV
                     KDAMGANMINSILESVANKLREWFPEEEILFSILSNFATESLASACCEIPFERLGRNK
                     EIGEQIAKKIQQAGEYAKLDPYRAATHNKGIMNGIEAVVAATGNDTRAVSASIHAYAA
                     RNGLYQGLTDWQIKGDKLVGKLTVPLAVATVGGASNILPKAKASLAMLDIDSAKELAQ
                     VIAAVGLAQNLAALRALVTEGIQKGHMGLQARSLAISIGAIGEEIEQVAKKLREAEKM
                     NQQTAIQILEKIREK"
     gene            complement(2867..3571)
                     /locus_tag="IWO89_002650"
     CDS             complement(2867..3571)
                     /locus_tag="IWO89_002650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0457346.1"
                     /translation="MKKKQKQKLLNQFRPSLDGVRGQLFRSLEEESLRRYGLPLQVML
                     DPKNRQILTIGLAGKTNEDTRINVPIDDNFNTVLKRIRSGENGIFERFRDNLLIEIVS
                     YWNDQRLKNDEPTAQAVATPVTEETVSVPEEKTEKPKADTTENTRSTDTNAALSFADF
                     SKAVAEYPKFYTEQNEHEVVINEKNADEPRKLATISMTTENEFVIEKALERKYKVKLT
                     LIPLIEQFAATAITDR"
     gene            complement(3758..4003)
                     /locus_tag="IWO89_002651"
     CDS             complement(3758..4003)
                     /locus_tag="IWO89_002651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0457347.1"
                     /translation="MDPIERLNSLSEEVTQTFHSDFVFLIDAEKIQHFPARNWTHDQI
                     IEELKKRFDHSLMVKPWHEHEVIYSPELPVFALIPKK"
     gene            complement(4020..4871)
                     /locus_tag="IWO89_002652"
     CDS             complement(4020..4871)
                     /locus_tag="IWO89_002652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HAQ0457348.1"
                     /translation="MNSLTSTYRLSNGYEIPVVGFGTWQTPDGDVAVSSVKEALAAGY
                     RHIDTAQGYKNEESVGQAIKESGIPREEIFLTTKLWNANHSYELVMSSFEESLKKLQT
                     DYLDLFLIHWPNPVAFRDNWEQANADTWRAFEELYEAGKIKAIGVSNFLPHHLDTLAK
                     TAKIMPMVNQVFLAPGELQTTVVEYAKKHDMILEAYSPLGTGKIFDVPEMKQIAEAHD
                     KTIAQVALRWSLQHEFLPLPKSVTPSRIKENTELFDFELTEEEMKQIDQLDGVVGKAK
                     DPDTTQF"
     gene            5037..6041
                     /locus_tag="IWO89_002653"
     CDS             5037..6041
                     /locus_tag="IWO89_002653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid-associated protein"
                     /protein_id="HAQ0457349.1"
                     /translation="MDIYLKKAILHIIDRETGSPVFSQKELDLTKEYIRDFLQKKIQK
                     ISSAQTKTGQLAEESMIVESLKIAQENFVEASEAIVQRWFDIYQESEEAPSADVFVVL
                     YELDTVMYLSLLKVNYREAYTHFVEADEAGIDNRLILNRAILGAKTQKADEAFAVNLT
                     DWTYELIEKKYEFSGKKELYFSGRVIESVPAPSLEENVKVIQKVAKHLGKKFETEEFD
                     IMADLKEAVYDTIEEKGRLDHEMIAEKVFKENITAKMAFKEEVQEQGFVPEAPPVKEV
                     QEISEKKFGKQKFKLSNGIELIVPVDVYRNPDLIEFVNNPDGTISVMIKNVDEVLNRL
                     "
BASE COUNT         1964 a         1063 c         1296 g         1808 t
ORIGIN      
        1 gattgttaaa atttaatctt agaaaaaaat aactggaaca agagctattt gtttttacag
       61 caaagaatgg tatatttttg taggaggtgg cttacttgaa agaagtcgtt atgatagatg
      121 ctgcaagaac gccgatagga aagtatcgcg gaagtttaag tccatttact gcagttgaat
      181 taggcacact tgtgactaaa ggattattag ataaaacaaa actgaaaaaa gataaaatcg
      241 atcaagtaat atttggaaat gtcctgcaag ctggtaatgg tcaaaatgtt gccagacaga
      301 ttgctttgaa cagcggtctc cctgtagatg tcccagcaat gacgatcaat gaagtttgcg
      361 gatctggtat gaaagcagtg atcttagcac gccagctgat ccaattaggt gaagcagaac
      421 ttgtgatagc cggcggaaca gagagtatgt ctcaagcacc tatgctgaaa cagtatcagt
      481 cagaaacaaa tgaatatggt gaaccaattt ccagtatggt caacgacgga ttgactgacg
      541 cattttcaaa tgcacatatg ggattaaccg cagagaatgt tgcaacacaa ttttctgtga
      601 gcagagaaga gcaggatcgc tatgccttgt cgtcccagtt gaaagcagca catgctgtcg
      661 aagccggtgt attttctgag gagatcatcc cagtcaagat ttctgatgaa gacgtgttat
      721 ctgaggatga agcagttcgt ggaaatagta cattggaaaa actgggcacg ttacgtacag
      781 tattctcaga agaaggaact gtaacagcag gaaatgcttc cccgttgaat gacggtgcct
      841 ctgtggtgat ccttgcatcc aaagaatacg cagaaaataa taatctgcct tatttagcaa
      901 ccatcaaaga agtagcggag gtcggtattg acccaagtat catgggaatc gctccaatca
      961 aagccattca aaaattgaca gatagatcag gaatgaattt atcaacaatc gatctttttg
     1021 agatcaatga agcatttgca gcttcttcta tcgtagttag ccaagaactg cagttagatg
     1081 aagaaaaggt caatatatat ggaggagcaa tcgcattagg ccatccaatt ggtgcaagcg
     1141 gtgcacgtat cttgactaca ttagcttacg gattgcttcg tgaacagaag agatacggta
     1201 ttgcttcatt atgtattggt ggaggtttag gcttggcagt cttgttagaa gcgaatatgg
     1261 agcaaacaca taaggatgtg caaaaaaaaa agttttatca gcttacgcca tctgaacggc
     1321 gcagtcaatt aatagaaaaa aatgtattga cacaagaaac tgcgttgatt tttcaggaac
     1381 aaacactttc tgaagagctc tccgatcaca tgatcgaaaa ccaagtgagt gaagtggaaa
     1441 tcccaatggg gatcgcgcag aattttcaaa tcaatggcaa gaaaaaatgg attccgatgg
     1501 caacagagga accatctgtc attgcagctg caagtaatgg tgcaaaaatc tgtggaaata
     1561 tctgcgcaga aacacctcag cggctgatgc gagggcaaat cgttctatca ggaaaatcgg
     1621 agtatcaagc agtgatcaat gcagttaatc atcgaaaaga agagctgatt ctttgtgcaa
     1681 atgaaagtta tccttcaatc gtgaaacgcg ggggcggtgt tcaagatatc tctactcggg
     1741 aattcatggg gagtttccat gcatacttat ctattgattt tttagtggat gtaaaagatg
     1801 caatgggcgc taacatgatc aactcgattc ttgagagtgt ggcaaacaag ttacgggagt
     1861 ggttccctga agaagagatt ctattcagta ttttaagtaa ttttgctaca gaatcattgg
     1921 cttctgcctg ttgtgaaatc ccattcgaac gcttagggag aaacaaagaa atcggtgaac
     1981 aaattgccaa aaaaatccaa caggcagggg aatatgcgaa gcttgaccct taccgtgcag
     2041 caacgcacaa caaagggatc atgaatggta tagaagctgt agtggcagct acaggaaatg
     2101 atacacgtgc ggttagcgct tcgatccacg cctacgcggc aagaaatgga ttataccaag
     2161 gattaacaga ctggcagatc aaaggggata aactagttgg aaaactaacg gtgcctttgg
     2221 ctgttgccac agtaggcgga gcttccaata tcctgccaaa agcaaaagca tctttagcta
     2281 tgttagatat tgattcagcc aaggaactag cacaagtaat tgctgcagta gggttagctc
     2341 aaaacctggc tgctttgcgt gcattagtta cagaaggaat ccaaaaaggc cacatgggac
     2401 ttcaagctcg ttcattagcg atctcaattg gggctattgg tgaggaaatc gagcaagttg
     2461 cgaaaaaatt acgtgaagca gaaaagatga accagcagac agctatacaa atattggaaa
     2521 aaatccggga aaaataaaaa agcaatatct ttagaagaat aaagcaagat ttacagttat
     2581 tttatttact gcagatcttg cttttttctc tatcagaaaa tatgtttatt attagaattt
     2641 tttatagtta aggttaggca tcttaagaaa tcaggcaact ccaagatatc ttcaggtaga
     2701 agagaagtcg cctaacattg taggatcata ggtgatttgt tggacaggtc aagctgatat
     2761 ttgagcctga tgttagaagg aatttctttt tacttgctgt agaaattatt atatgactaa
     2821 aaaggctggg attttaaatc ccagcctttt atgacaaata gaatttttat cgatcagtaa
     2881 tcgctgtcgc tgcaaattgt tcgatcaaag gaatcaaagt caatttgact ttgtatttcc
     2941 gttcgagtgc tttttcgata acgaactcat tttcagtagt catagaaata gttgctaatt
     3001 tacgaggttc atcagcattt ttttcattaa taactacttc atgttcattt tgttctgtgt
     3061 aaaatttagg atactcagca acggcttttg aaaaatcagc aaaacttaaa gcggcattcg
     3121 tatcagttga gcgtgtattt tcagttgtgt ccgcttttgg tttttccgtt ttttcttctg
     3181 gcacactgac tgtttcttct gtaactggtg tcgcaacagc ttgcgcagtt ggttcatcgt
     3241 ttttcaatcg ttgatcattc cagtaagaca cgatttcaat aagaagattg tcacggaatc
     3301 gttcgaaaat cccgttttct cctgaacgaa ttctctttaa tacagtattg aagttgtcat
     3361 cgattggcac gttgatccgt gtatcttcat ttgttttccc agctaatccg atcgttaaga
     3421 tttgacggtt tttgggatca agcatgactt gtaacggcaa accataacgt cgcaagctct
     3481 cttcttctag agaacggaat aattgtcctc ttacaccatc caatgaagga cggaattgat
     3541 ttaataattt ttgtttctgt tttttcttca tatgttctct ccttcgctca taccctttcg
     3601 attataagaa aaaaacagcc aaatgaaaag gagtaatgca aaaaaacttg cgtatacgca
     3661 aattacaaca gaaaaatttg cgtattttcc cgttttttga aagaaaaata aaaaatggaa
     3721 cggattatgt atatatacat aatccgttcc attctttcta ttttttcggt atcaaggcaa
     3781 aaacaggtag ttcaggtgaa taaatgactt catgttcgtg ccagggtttg accattagcg
     3841 aatgatcgaa ccgttttttc agttcttcga tgatttgatc atgtgtccag tttcttgcag
     3901 gaaaatgttg gattttctct gcatcgatca aaaagacaaa atcggaatga aaagtctggg
     3961 taacttcttc cgagagagaa ttcaagcgtt cgattggatc cataaaatca cctttcgact
     4021 taaaattggg tagtatctgg gtcttttgct ttgccgacta caccatctaa ctgatcgatt
     4081 tgtttcattt cttcttctgt taattcaaaa tcaaataatt cagtattttc tttgattctg
     4141 cttggtgtca ctgacttagg aagaggaagg aattcgtgtt gcagcgacca acgcaaagca
     4201 acttgtgcaa tcgtcttgtc atgtgcttca gcgatttgtt tcatttcagg cacatcgaag
     4261 attttaccag tgcctaatgg actatatgct tcgaggatca tatcatgttt ttttgcatac
     4321 tcaactacag ttgtctgtaa ttcgcctggt gcaaggaaaa cttgattgac catcggcata
     4381 atttttgctg tttttgctag tgtatctaga tggtggggta agaaattgct cacaccgatt
     4441 gctttgattt tacctgcttc atacagttct tcaaatgctc gccatgtatc tgcatttgct
     4501 tgttcccaat tatcacgaaa agcaacaggg tttggccagt ggatcaaaaa cagatctaaa
     4561 taatctgttt ggagcttctt caatgattct tcaaaagaag acatcacaag ttcatagctg
     4621 tggtttgcat tccataattt tgttgtcaag aatatctctt ctctaggaat tccactttcc
     4681 ttaatcgctt ggccgacgct ttcttcattc ttgtatcctt gggcagtatc gatatggcga
     4741 taacctgcag ccagcgcttc tttaacagag gaaacagcga catccccatc aggtgtttgc
     4801 catgtgccga atcctactac cgggatttcg taaccatttg atagacgata agtgcttgtt
     4861 aatgaattca tactttcagc tcctttttta ttcgttcaac tataagtgta gcgcattttt
     4921 cgaaaaaaag aatccatttt gtttctactc ttgaaatttt tgtttaaaag aaaatgattt
     4981 tgattctggt atttttgatt ttttttccgt aaactaagac agaagggaat gagaaaatgg
     5041 atatctattt aaaaaaagca attctgcata ttattgacag agaaactggt tctccagtct
     5101 tttcacaaaa agagctggat ctgacaaaag aatatattcg tgattttctt caaaagaaga
     5161 tccaaaaaat ctcttctgct caaacaaaaa ctggacaact agcagaagaa agcatgatcg
     5221 ttgaatcact taaaatcgct caagaaaatt ttgtggaagc aagtgaagca atcgttcagc
     5281 ggtggttcga tatctaccaa gaaagtgaag aagcacctag tgcagatgta tttgttgtct
     5341 tgtatgaatt agatacagtg atgtacttat ctttattgaa agtcaattat cgtgaggctt
     5401 acactcactt tgttgaagca gacgaagcag ggatcgacaa ccgcttgatt ttgaatcgag
     5461 caattctagg ggcgaaaaca caaaaagcag atgaagcatt cgctgtgaat ttgactgatt
     5521 ggacctacga attaattgaa aaaaaatatg agttttctgg caaaaaagag ctttattttt
     5581 ctggaagagt gatcgaaagt gttcctgcac cttctttaga agaaaatgta aaagtgatcc
     5641 aaaaagtagc caagcatctt ggaaaaaaat ttgagacaga agaatttgat atcatggctg
     5701 atttgaaaga agctgtctat gatacgatag aagaaaaagg tcggttggat catgagatga
     5761 ttgcggaaaa agtcttcaag gaaaacatca cggcaaaaat ggcttttaaa gaagaagtac
     5821 aagagcaagg atttgtccca gaagcacctc ctgtaaaaga agtacaagaa atttctgaga
     5881 aaaaatttgg aaaacaaaaa ttcaagctat caaacgggat cgaactgatc gtaccagtag
     5941 atgtatatcg caacccagat ttgattgaat ttgtgaataa tccggatggt acgatatccg
     6001 tgatgatcaa aaatgtggat gaagtattga atcgtttata aaaatgacta gaatattagg
     6061 tgttcaagta ccagtgatca gttaaatgaa ttgtgtcaag agaaggatcg gaaaagacga
     6121 atataacaaa a
//
LOCUS       DACEEZ010000124         6097 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20313_2017
            SAMN12639966-rid9879223.denovo.127, whole genome shotgun sequence.
ACCESSION   DACEEZ010000124 DACEEZ010000000
VERSION     DACEEZ010000124.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639966
            Sequence Read Archive: SRR10023158
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6097)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6097)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741683.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741683.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:44:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,016
            CDSs (total)                      :: 2,962
            Genes (coding)                    :: 2,889
            CDSs (with protein)               :: 2,889
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 36 of 73
            Pseudo Genes (incomplete)         :: 29 of 73
            Pseudo Genes (internal stop)      :: 18 of 73
            Pseudo Genes (multiple problems)  :: 8 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6097
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639966-rid9879223.denovo.127"
                     /isolate="E20313_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            54..947
                     /locus_tag="IWO89_002654"
     CDS             54..947
                     /locus_tag="IWO89_002654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1803 domain-containing protein"
                     /protein_id="HAQ0457350.1"
                     /translation="MNYYSKNKENFYQKLTDDPLFSSLTDYLYEHREQETILRELKKE
                     FSQNKFSHFLDLLIDAGLIKREERRYHLNFPIFDPKDYLQQATSAAETIAEQLKRLSV
                     DEQKLAMGEVIWAYCFEDERKEAYFYGVRNSRETELLRTTAGNEKYRFITLSSIEHFP
                     LTLANYFFVQKNQLPVTKAFKELAELIGDVNESYFFDQIEVIVDRIRKNKYKNRRPSI
                     FHQSLLVTNTIKEEESFTLELPIVEKNNFEIELPTLDPSLTMEEMAFLKRQIFSELSK
                     KFIPHAFSYIKEYGTVLVSKT"
     gene            1081..1206
                     /locus_tag="IWO89_002655"
     CDS             1081..1206
                     /locus_tag="IWO89_002655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAQ0457351.1"
                     /translation="MQKNGEKCGMTKEVVIRKVRFLNNQYYDSVKYGILWEELAD"
     gene            complement(1340..1972)
                     /locus_tag="IWO89_002656"
     CDS             complement(1340..1972)
                     /locus_tag="IWO89_002656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0457352.1"
                     /translation="MEIQRRTTNFLHLGSFFATLTILFFLCYSYLKTSHAPSSALGWL
                     LLDHSDITEAEENPFFLILSSLCGLLFSVYFGAYFFHRHPGTLNEASSVLIAFKTFSF
                     LAILFYSFNHNTLFFFIMNGLVVILSIFTYFIFSLIFSQLKCPLFFRLVPVTCFIYSL
                     FSFFVLALMPLTNPSTIQVAGNLLDMLFIVSLGVFMWQRKNKQNKKHFEA"
     gene            complement(2120..2662)
                     /locus_tag="IWO89_002657"
     CDS             complement(2120..2662)
                     /locus_tag="IWO89_002657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2179 domain-containing protein"
                     /protein_id="HAQ0457353.1"
                     /translation="MIDPKMLGMIFLINFAYITLNTLRFMLTMKGYRLIAPLVSMVEI
                     TIYIVGLSLVLDRLDNPLNLFVYALGYAVGISVGILIEDKLALGYIMVTTILPANSAE
                     DKSLPGILREHGYGVTQTSAMGLEGERLVLEILSPRKSERELYRLIKEIEERAFIISY
                     EPKYISGGFWTKKVRKRQGK"
     gene            2797..3186
                     /locus_tag="IWO89_002658"
     CDS             2797..3186
                     /locus_tag="IWO89_002658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0457354.1"
                     /translation="MKTVNRVFCASHVLSLLLSIYLGVYSIQQPIEVENIVFQMSLTD
                     GLILSVFFFLILFIGNIFGSVASFLNFSIYPLLSLALGVVGLCSLCLFPEPFSPAFIL
                     FGTLNLFQTTVGAWLLWRSGNLMKIGE"
     gene            3190..3954
                     /locus_tag="IWO89_002659"
     CDS             3190..3954
                     /locus_tag="IWO89_002659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HAQ0457355.1"
                     /translation="MNPIILKRSVHHIPVLEVVLKELREERIPVVIYYHGWQTSKELV
                     LTQGRKIAQQGIRVILPDAMNHGERKQPVSSIPSYTFWDSIYGNLFEFDTLIKHLEKR
                     GLLHDQLAVGGVSMGGMTTCALLAKHPHIVAGVCLMGSPAPLEYGKEIVRRASEYNYV
                     VPKDYFDLISWVKSYDLSLQPEKLAGRPLFFWHGQADEKIPYIQTERFVEKNRHKSYG
                     KEIVFRSSKTQRHMVQIPLMEEASTFLAKNLIKKYK"
     gene            4083..5030
                     /locus_tag="IWO89_002660"
     CDS             4083..5030
                     /locus_tag="IWO89_002660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family transcriptional regulator"
                     /protein_id="HAQ0457356.1"
                     /translation="MPTLADVAKRANVSKMTVSRVINHPEQVTDELKELVFSAMAELD
                     YRPNIAAKALVTNRSQIIKLFILEEIDTTEPYYMNLLMGIAKRIGKAHYSLQLVTNDA
                     FDVGSCDGYIITGVRKKDFDWIDRLEKPVVLFGENSEGYDYVDSDNEYGTAKATQYAL
                     ERDYQTIIYIGIEVDEPFEISRETGYLSVMKEVDLPSKILRFPNHSTEVERYMTENWA
                     DFTDKTCFVCSSDRLALGAVRGIINAGGHLPEEFGVIGFDGVFLDQISSPKLTTIKQD
                     IIRMGEVCGEMLLKKIEEKGASQGYRRFFPELVIRETTN"
     gene            5160..5900
                     /locus_tag="IWO89_002661"
     CDS             5160..5900
                     /locus_tag="IWO89_002661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0457357.1"
                     /translation="MKEIRRKHFRFPAYDDELGVKLTNDKKRVLFDGQEDLLTEQNQS
                     PFEQMKEFHFTNTNTEKIRRKRRATNGLKSAPRKENLEKHKGKLPDYTGHYQSEITST
                     GRKKLFGDSSRSVRQSETRTESKHTTRNASVDTKVRERSYFVPKYVPASIIPDPRESK
                     ISEDHLIESMKKTDDSYLLFDTEPTPYQEKRNGEPSVRTFNRTKTLEETKQSEEKKSK
                     KSHGILERSLQGLIDDQGNSLGENSYFK"
BASE COUNT         2144 a          987 c         1192 g         1774 t
ORIGIN      
        1 ttttacgtaa aagcctttta agtaaaataa aacggataga aaagagaaca tatatgaact
       61 attattcaaa aaataaggaa aacttttatc aaaaactgac cgatgatccg cttttttctt
      121 cattgaccga ttatctatat gaacatcgag aacaagaaac gattctaaga gaattgaaaa
      181 aagaattttc gcaaaataaa ttttcccatt ttcttgattt attgatcgat gcaggattaa
      241 tcaagaggga agaacgaaga tatcatttga actttccaat atttgatcct aaagattact
      301 tgcaacaagc cacttcggct gctgaaacca tagctgaaca attgaagaga ctctctgttg
      361 atgaacaaaa attagcgatg ggagaagtta tttgggcgta ttgtttcgaa gatgaacgga
      421 aagaagcgta tttttatgga gtaagaaaca gccgagagac agaattgctg aggacaactg
      481 caggaaatga gaagtaccgt tttatcacac tttcctctat agaacacttc ccgcttactt
      541 tggctaatta cttttttgta cagaagaatc agcttcctgt aacgaaggca ttcaaagaat
      601 tagccgaact tattggagac gtaaatgaat cgtacttttt tgatcaaata gaagttatcg
      661 ttgacaggat acgtaagaat aaatataaaa atcgccggcc tagtattttc catcagtctc
      721 tacttgtgac gaatactata aaggaagaag aatcattcac attagaatta cctattgtcg
      781 aaaaaaacaa ttttgagatt gaattaccaa cattggatcc ttcattgaca atggaggaaa
      841 tggcctttct taaacgacaa atcttttctg agctgagcaa gaaatttata ccgcatgcat
      901 tttcttatat caaagaatat ggaacagttt tagtgagtaa aacctaagag agcagtaagc
      961 aaaaattata ctgactcttg caggaggagc ctttctcttc ctttatggtt aaaagaatta
     1021 tttgagtatt ttgcctatct accacctatt agattcacta catggtctgg aaacaaagga
     1081 atgcaaaaaa atggtgagaa atgtggaatg acaaaggaag tagtgataag aaaagttaga
     1141 tttttaaata atcaatatta tgattcagtc aagtatggca ttttatggga agaactagct
     1201 gattagatta ggaagtccat ttgataatcc agaatcacta agaaacgaat ggcgagtcat
     1261 taataattta tccaatcttg ggttattctt ttagattgaa gtgtttaaaa ctaaaaaagc
     1321 atttatgaag tcgtaatagt caggcttcaa aatgcttttt attttgtttg ttttttcttt
     1381 gccacataaa tacacctaga ctgacgatga agagcatatc caacagattt cctgcgactt
     1441 ggatcgtcga agggttggtc agtggcatta aagcaaggac aaaaaaggaa aacaagctgt
     1501 aaataaaaca ggtgacagga actaatcgaa agaagagcgg acatttcaat tggctaaaaa
     1561 tgagtgaaaa aataaaataa gtaaaaatgg ataaaatcac gactaaacca ttcatgataa
     1621 agaagaaaag cgtattgtga ttaaacgaat aaaaaagaat tgctaaaaaa gaaaacgttt
     1681 taaatgcaat caatacgctt gaagcttcgt ttaacgtacc aggatgtcga tgaaaaaaat
     1741 aggcaccgaa ataaacagaa aacagtaagc cgcaaagaga agacaaaatc aaaaaaaacg
     1801 gattttcttc ggcttctgtg atatcagaat gatccaaaag caaccagcct aatgcactag
     1861 aaggcgcatg tgaggtctta aggtatgaat aacatagaaa aaataagatc gtcaaagttg
     1921 caaaaaaaga gcctaaatgt aaaaaattag tggttctacg ttgaatttcc ataatatctc
     1981 caaattaaat tgataagtat ataaagtata ataacaaatt gatgagtata tgtcgatgtt
     2041 ttttaatcaa aataataaat aaaaactgtc taacatttaa aatttaattt agatattaga
     2101 cagtttttaa gtttgtgagt tattttcctt gtcgttttct cactttttta gtccagaaac
     2161 cacctgaaat atattttggc tcataagaaa tgataaaagc acgctcttcg atttctttga
     2221 tcaggcgata cagttcacgt tcgctttttc taggagaaag tatttcgaga actagccgct
     2281 caccttccag ccccattgca gaagtctgtg tcacaccata accgtgttct ctcagtatac
     2341 ccggcaaact tttatcttct gcgctatttg ctggcaaaat cgttgtgacc atgatgtatc
     2401 ctaatgccaa tttgtcttca atcaaaattc cgacactgat acctactgcg taacctaacg
     2461 catagacaaa taaattcagt ggattatcta aacgatctaa aacaagggat aagccgacga
     2521 tataaatcgt gatctcgacc atactgacaa gtggagcaat cagtcgatag cctttcattg
     2581 tcaacatgaa acgtaacgtg ttcagagtga tataagcaaa attaatgagg aagatcatcc
     2641 ccaacatttt tggatcaatc atttgtaaaa ctcctttcat ttatataaca acaatatcaa
     2701 aaaaaatcgt aagtgaagaa aaattgaaac tttatacata caaaaaaatg ataaaatgat
     2761 tataatagaa ttataaagat aggttaggtg attaaaatga aaacggtcaa tcgtgtgttt
     2821 tgtgccagcc acgtgctaag tctccttctc agtatatact taggtgtcta ttcgattcaa
     2881 cagccaattg aggtagagaa cattgttttt cagatgtcgc tgacagatgg tttaatttta
     2941 agcgtttttt tctttttgat actgtttatt ggtaatattt tcggtagcgt tgccagtttt
     3001 ttgaattttt ctatttatcc actcctttcg ctagcattag gagtggtggg actttgttct
     3061 ctgtgcttat ttcccgagcc attcagtcct gcatttatat tatttggtac actgaacctg
     3121 ttccagacaa ctgttggagc atggttgcta tggcgatcag gaaatttaat gaaaataggt
     3181 gagtaacaat tgaatccaat tatattgaaa cgttctgtcc atcatattcc agtgttagaa
     3241 gttgttctga aagaattaag agaggaacgt attcccgttg tcatctatta tcacggatgg
     3301 cagacaagta aggagttagt tttgacgcaa ggaagaaaaa ttgcgcagca gggcattcgt
     3361 gtgatcctgc cagatgcgat gaatcatggg gagcgcaaac agccggtttc ttctattcca
     3421 tcctatacat tttgggatag tatctacgga aatctgtttg aatttgacac acttatcaag
     3481 catttagaaa aaagaggtct tctacatgat cagcttgcag ttggcggggt ttcgatgggg
     3541 ggaatgacaa catgcgcctt gttggctaaa catcctcata tagtagcggg cgtgtgtttg
     3601 atgggttccc ctgcaccttt ggaatatgga aaagaaatcg taaggcgcgc atcggaatat
     3661 aattatgtcg taccaaaaga ctattttgat ttgatttcat gggttaagag ctatgatttg
     3721 tcattgcagc cggaaaagtt agctggacgt cctctgttct tttggcatgg acaagctgac
     3781 gaaaaaatcc cttatataca aacggaacga tttgtagaaa aaaatcggca taagtcttac
     3841 ggaaaagaaa ttgtttttcg ttcctcaaaa acacagcgac atatggtaca aataccacta
     3901 atggaagaag cttccacttt tttagcaaaa aatttgataa aaaagtataa ataattgaaa
     3961 cggaaaatca ctcatgacga tagaggattt tccgtttttt attttgggct ataataaact
     4021 ctgttaggaa aatttggtat aattgatagt aagaatgaca acatgttaga aaggaagaga
     4081 gcatgcccac tttagcagat gttgcaaaac gtgccaatgt ttcaaaaatg acggtctctc
     4141 gagtcatcaa tcatccggaa caggtgactg acgaattgaa agagttagtt ttctcagcaa
     4201 tggctgaact ggattaccgt ccaaatattg ctgcaaaagc acttgtcacg aatcggtcgc
     4261 agatcatcaa attatttata ctggaagaaa tcgacacaac tgagccctat tacatgaatt
     4321 tgctgatggg aatcgctaaa cggatcggta aagcgcatta ttctctccaa ttagttacaa
     4381 atgatgcctt cgacgtcggt tcttgtgatg gatacatcat cacaggtgtc agaaaaaaag
     4441 acttcgattg gatcgaccgt ttggaaaaac cagttgtctt gtttggggaa aattctgaag
     4501 gatatgatta tgtagacagc gataatgaat atggtacagc aaaagcaacg caatatgcct
     4561 tagaaagaga ttatcaaacg attatttata tcggtatcga agtggacgag ccttttgaaa
     4621 tttcaaggga aactggttat ttatctgtga tgaaggaagt tgatcttcct agtaaaatct
     4681 tgcggtttcc aaatcattcg acagaagtag aaagatatat gacagaaaac tgggcagatt
     4741 ttacagataa aacttgtttt gtatgtagtt cagatcggct agcactagga gcagtaagag
     4801 ggattataaa tgctggagga cacttgcccg aagaatttgg tgtgattgga tttgatgggg
     4861 tatttttaga ccaaatcagt tcacctaaat tgacaacgat caagcaagat attatacgta
     4921 tgggtgaagt atgtggagaa atgttgttaa aaaaaataga agaaaaaggg gcgagtcaag
     4981 gttatcgtcg tttttttcct gaactagtta tcagagagac aacgaattaa gtcttattac
     5041 tatatatata tttatgaaat tcatcgctat tttcttaaaa taatcagcgc attgctactg
     5101 aaaacaatga atctgtgcta ttattgagaa aagttccatt tttgagaagg atggtttgaa
     5161 tgaaagaaat cagacgaaaa catttccgtt ttcctgcgta cgatgatgag ttaggcgtaa
     5221 aattaaccaa cgacaagaag cgagtattat ttgacggaca agaagatctt ttgactgaac
     5281 agaatcagtc cccgtttgaa cagatgaaag aatttcattt cacgaacacg aacaccgaga
     5341 aaatccgtcg caagcgtcgt gcaacaaatg gattgaagtc agctccgcgt aaggaaaatt
     5401 tggaaaagca taaaggaaaa ttaccggatt ataccggcca ttaccaatca gaaatcacat
     5461 cgacaggacg aaaaaaactt tttggtgatt cctctcgttc tgtcagacaa agtgaaacaa
     5521 ggacagagtc caagcatact acacgaaacg catctgttga cacgaaggtg cgggagcgtt
     5581 cttattttgt tcctaaatat gtacccgcat cgattatccc agaccctaga gaaagcaaaa
     5641 tttctgagga tcatttaatc gaatcgatga aaaaaacgga tgactcttat ctgttgttcg
     5701 atacagaacc gacgccgtat caagaaaaaa gaaatggtga gccgtctgta cgaacattca
     5761 atcgaacaaa aacgttagag gaaacaaagc agtcagaaga aaaaaaatcg aaaaaaagcc
     5821 atggtatctt agaacgctca ttacaaggat tgatcgatga tcaaggaaac tctttgggag
     5881 aaaatagcta ttttaaataa gtgatataca aatttatatt cggctacagt tggaaaggaa
     5941 acgctgcggc gtttccttat tttatactct atccgaaagt ttatatcgta tcctcaaatt
     6001 tttttgagtt taagcgaatt ttttctccca ataagtaaga aaataatggc aaaataaaaa
     6061 aacaaactat tttcgcagat cgtttgcaaa aatagtt
//
LOCUS       DACEEZ010000125         6053 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20313_2017
            SAMN12639966-rid9879223.denovo.128, whole genome shotgun sequence.
ACCESSION   DACEEZ010000125 DACEEZ010000000
VERSION     DACEEZ010000125.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639966
            Sequence Read Archive: SRR10023158
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6053)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6053)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741683.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741683.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:44:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,016
            CDSs (total)                      :: 2,962
            Genes (coding)                    :: 2,889
            CDSs (with protein)               :: 2,889
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 36 of 73
            Pseudo Genes (incomplete)         :: 29 of 73
            Pseudo Genes (internal stop)      :: 18 of 73
            Pseudo Genes (multiple problems)  :: 8 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6053
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639966-rid9879223.denovo.128"
                     /isolate="E20313_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            <1..1005
                     /locus_tag="IWO89_002662"
     CDS             <1..1005
                     /locus_tag="IWO89_002662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAQ0457358.1"
                     /translation="KELVKEGKSIILITHKLDEIKAVADRCTVIRRGKGIGTVNVKDV
                     TSQQLADMMVGRTVSFKTMKKEAKPQEVVLSIENLVVKENRGLEAVKNLNLEVRAGEV
                     LGIAGIDGNGQSELIQALTGLRKAESGHIKLKGEDITNKKPRKITEHGVGHVPEDRHK
                     YGLVLDMTLSENIALQTYHQKPYSKNGMLNYSVMNEHARELIEEYDVRTTNELVPAKA
                     LSGGNQQKAIIAREVDRDPDLLIVANPTRGLDVGAIEFIHKRLIEQRDKYKAVLLISF
                     ELEEILNVSDRIAVIHEGEIVGIVDPKETSENELGLLMAGYTLEEARKELEKGAGAHE
                     "
     gene            998..2131
                     /locus_tag="IWO89_002663"
     CDS             998..2131
                     /locus_tag="IWO89_002663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAQ0457359.1"
                     /translation="MNDRSEKFRNLLVPVLSVVLGLILGAILMAAFGFQPIQGYSSML
                     DAALGSQRGIGETLRQATPLIFTALGFSVANSAGFFNIGLSGQALCGWIVSVWVALAF
                     PDLPKVVLLPLCVILGALAGAMAAAVPGLLRAFFGTSEVIVTIMMNYILLYVSTFALH
                     EVMPESYRSSLDSSNMISQNASLRLPWLTQAFGGSRVNSGLFLAIIALIFVWLLMKKT
                     TLGFEIRSVGLNPFASEYAGMSSKRTIVLSMVISGALAGLGGVVEGLGTYQNFFVQTT
                     SLSIGFDGMAVSLLGSGSAIGILLSAILFSILKIGGLGMQTGAGVPFEIVNVSIALII
                     FFVGINFLIRFVMAKVFQGKKQEEIVATIESKPTNQNHEGGEL"
     gene            2131..3087
                     /locus_tag="IWO89_002664"
     CDS             2131..3087
                     /locus_tag="IWO89_002664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAQ0457360.1"
                     /translation="MSTIELIALILTSTLVYSTPLILTSLGGTFSERSGIVNVGLEGI
                     MVMGAFSSIVFNLTMSPSLGSMTPWIAILVGGVVGILFSLIHAVATVSLRADHIISGT
                     VINLMAPALGVFLIKAWYGKGQTDNITQNLGYFSFPGLADIPVIGPIFFKNTFLPAYC
                     AILIAVLAWVIIFKTKFGLRLRSVGENPQAADTLGINVYAMRYSGVLISGLLGGMGGA
                     VFAQSISGNFSAGTIVGQGFISMAAMIFGKWNPLGAMGASLFFGFAQSLSIIGAQLPV
                     ISSIPPVLLQIAPYLLTIIVLVVFLGKASGPKANGKNYIKSK"
     gene            3186..4352
                     /gene="deoB"
                     /locus_tag="IWO89_002665"
     CDS             3186..4352
                     /gene="deoB"
                     /locus_tag="IWO89_002665"
                     /EC_number="5.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopentomutase"
                     /protein_id="HAQ0457361.1"
                     /translation="MFKRVHLIVMDSVGIGEAPDADKFGDVGSDTLGHIAKEAGLNVP
                     HLEQLGLGTIRPLEGVESVKDHQGYATKLEEVSVGKDTMTGHWEIMGLNIKTPFRVFP
                     NGFPDDLIKQIEEFSGRKVVCNKPYSGTAVIDDYGEHQMKTGDLIVYTSADPVLQIAA
                     HEEIIPLEELYRICQFVRDITKDDPYMIGRIIARPYVGEPGDFTRTSNRHDYALNPFG
                     HTVLDSLKEAGKDVIAVGKINDIFNGQGITESVRTKSNMDGVDQLLKVMKNDFSGISF
                     TNLVDFDALYGHRRDVVGYAHAIEEFDLRIPELLEAMEEDDLLMITADHGNDPTFTGT
                     DHTREYVPLLVYSKKMKEQGALPQGYYSDISATIAENFSVSSTENGESFLKLLK"
     gene            4376..5194
                     /locus_tag="IWO89_002666"
     CDS             4376..5194
                     /locus_tag="IWO89_002666"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HAQ0457362.1"
                     /translation="MTKLSEMLQETTQFILDKGVKEIDFGLILGSGLGELADEIEDAV
                     MISYDEIPFFPVSTVVGHAGKLVYGSLAGKKVLAMQGRFHYYEGHSMQTVTYPVRVMA
                     GLGAHSVIVTNACGGVNESFVPGDLMLITDHINFTGENPLIGPNEDAMGPRFPDMSQA
                     YTPDYQATAKAAAEKLGLDLKEGVYMGYSGPTYETPAEIRMSRTIGADAVGMSTVPEV
                     IVAAHSGLKVLGISCITNLAAGMQANLSHEEVVETTQRVKQSFKALVKETLTLL"
     gene            5211..5918
                     /gene="deoD"
                     /locus_tag="IWO89_002667"
     CDS             5211..5918
                     /gene="deoD"
                     /locus_tag="IWO89_002667"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HAQ0457363.1"
                     /translation="MSVHIEAKEGEIADKILLPGDPLRAKYIAETFLEDPVCYNQVRG
                     MLGFTGKYKGERISVQGTGMGMPSATIYAHELIQSYGVKKLIRVGTCGALSKDVHVRD
                     LVLAQGAATSSSMIEKNFQAFHFPPISDFNLLLKAYEIAKEKGYTVHVGNVLSEDSFY
                     KDDLTETFQLAELGVLGVEMEAAALYYLGAKYHVQTLSLMTVSDHLITGEETTAAERQ
                     STFNEMIEVGLETAIAE"
BASE COUNT         1799 a         1086 c         1423 g         1745 t
ORIGIN      
        1 aaggaattag tcaaagaagg caagtcgatc attttgatta cgcataagtt agatgaaatc
       61 aaagcagtag ctgaccgctg tacagttatc cgccgtggaa aaggaatcgg tacagtcaac
      121 gttaaagacg taacctcaca acaattagcc gatatgatgg ttggaagaac ggtttcattc
      181 aaaacgatga aaaaagaagc gaagcctcaa gaagtcgttt tgtctattga aaatctagtg
      241 gtgaaagaaa atcgtggatt agaagccgtg aaaaacttga acttagaggt tcgtgctggc
      301 gaagtacttg gtatcgctgg aatcgatgga aacgggcagt cggagttgat ccaagctttg
      361 actggtttgc gaaaggcaga aagcggacat atcaagctaa aaggggaaga catcactaat
      421 aaaaaacccc gaaaaatcac tgaacatggt gtaggacacg tgccagaaga ccgtcataaa
      481 tacgggttag tcctagatat gacattatct gaaaatatcg cactacaaac ctatcatcaa
      541 aaaccttaca gtaaaaacgg catgctgaat tattcagtaa tgaatgaaca tgccagagaa
      601 ttgatcgaag aatatgatgt tcgaacaacg aatgaacttg ttcccgcaaa agctttatca
      661 ggcggaaatc agcaaaaagc aattatcgct cgtgaagtcg accgagatcc tgatctgttg
      721 attgttgcaa atccaactcg tggactggat gtaggtgcga tcgaatttat tcataaacgt
      781 ctgatcgaac aaagggacaa atacaaagca gtgttactga tcagtttcga attagaagaa
      841 attttaaatg tttcggatcg tattgctgtt atccatgaag gagaaattgt cggtatcgtt
      901 gatccgaaag aaacatctga aaatgaatta ggtctattga tggcgggcta taccttagaa
      961 gaagcacgaa aagagttaga aaagggggct ggcgcacatg aatgatcgca gcgaaaaatt
     1021 ccgtaattta ttagtgcctg tactttcagt tgttttagga ttgatcttag gcgcgatatt
     1081 gatggctgct tttggttttc agcccatcca aggttattct tctatgctag atgcagccct
     1141 tggttcgcaa agagggatag gtgaaacgtt aaggcaagca acgccgttga tttttacagc
     1201 attaggtttt tctgtagcta actcagctgg gtttttcaat attgggcttt ctggacaagc
     1261 actttgcggg tggatcgtca gtgtctgggt ggcacttgcg tttccagatc tgccaaaagt
     1321 tgttttgctt ccgttatgcg tgatcttagg tgccttagct ggtgcgatgg cagcggctgt
     1381 tccaggttta cttcgagcat tttttggaac aagtgaagtg atcgtaacaa tcatgatgaa
     1441 ctatatctta ttatacgtaa gcacatttgc tttacatgaa gtgatgcctg aaagctatcg
     1501 ctcaagtttg gactcttcca atatgatcag tcaaaatgcc tcactgcggt taccatggtt
     1561 aacacaagct tttggcggct ctcgcgtaaa ctcaggatta tttttagcaa tcatcgcgct
     1621 tattttcgtt tggctgctga tgaagaaaac gactttagga tttgaaatcc gttctgttgg
     1681 attgaatcct tttgcttctg agtatgcagg aatgagtagt aaacgtacga tcgttttgtc
     1741 gatggtcatt tctggcgctt tggcagggct tggcggtgtg gttgaaggat taggaacgta
     1801 tcaaaacttc tttgttcaaa cgacttcgct atctattgga tttgacggaa tggctgtttc
     1861 cttgcttggt tctggatcag caatcggtat tcttttatca gcgatcttgt tcagtatttt
     1921 gaagatcggt ggacttggca tgcagacagg agcaggtgtt ccgtttgaga tcgtcaatgt
     1981 atcgattgct ttgattatct tctttgtcgg gatcaacttc ctgattcgtt tcgtcatggc
     2041 taaagtcttc caagggaaaa aacaagaaga gatcgttgcg acaatagaat cgaaaccaac
     2101 gaatcaaaac catgaagggg gcgaactata atgtcgacta tcgaattaat tgccttgatc
     2161 cttacttcta cactggttta ttcaacaccg ctaattctaa cgtcactagg cggaactttt
     2221 tctgaacgaa gcgggatcgt aaacgtaggg ctagagggaa tcatggtcat gggtgctttt
     2281 agttccatcg ttttcaactt gacgatgagt ccatcacttg gttctatgac accatggatc
     2341 gctattttag tgggtggcgt cgtaggtatc ctattttctc tgattcacgc agtagcaaca
     2401 gtcagtttgc gtgctgacca tattatcagc gggacagtaa ttaatctgat ggcacctgct
     2461 ttaggtgttt tcttgatcaa agcatggtat ggtaaaggtc aaactgataa catcacgcag
     2521 aatctggggt atttttcttt cccaggatta gcagatattc cggtcattgg accaattttc
     2581 tttaaaaata cattcttacc tgcttattgt gctattttga ttgctgtcct agcttgggtg
     2641 atcatcttca agacgaaatt cggcttgcgc ctccgttcag ttggtgaaaa tcctcaagct
     2701 gccgacacat taggtatcaa tgtatatgct atgcgttatt caggcgtact gatttctggc
     2761 ttgttaggcg gtatgggcgg tgctgttttt gctcagtcga tttccggtaa cttttcagct
     2821 ggtacgatcg ttggacaagg gttcatctcc atggctgcta tgatctttgg taagtggaat
     2881 cctttagggg caatgggtgc ctcattgttc tttggttttg ctcaaagctt gagtatcatt
     2941 ggcgcacaat taccagttat ttcttcgatt ccgccagtat tgctgcaaat tgctccatat
     3001 ttgctgacaa tcattgtgct ggttgttttc ttagggaaag cttctggtcc aaaagcaaac
     3061 gggaagaact atatcaaatc aaaataatct tttagcgaat aaaaagggtg ggttacggga
     3121 agtgtttttc gtctcgccct ttttgtttta aaggcaataa aaataaaaag aaagaaggaa
     3181 taatcatgtt taaacgcgta catctgatcg tcatggattc agtaggaatt ggagaagcac
     3241 cagatgccga taagttcggt gatgttggtt ctgatacatt aggacacatt gcgaaagaag
     3301 caggattgaa cgttccacat ttagagcagc ttggtttagg aacgatccgt cctcttgaag
     3361 gtgtggaatc agtgaaagat catcaaggct atgcgacgaa attagaagaa gtttctgtag
     3421 ggaaagatac gatgacaggc cactgggaaa tcatgggatt gaatatcaaa actccatttc
     3481 gtgtgtttcc taatggattc cctgatgatc tgatcaaaca aatcgaagaa ttttctggcc
     3541 gtaaagtcgt atgtaacaaa ccttatagcg gaacagctgt tattgacgat tacggtgaac
     3601 accaaatgaa aacaggcgat ttaattgtat atacttctgc cgatcctgtc cttcagatcg
     3661 cggcacatga agaaatcatt cctttagaag aattatatcg gatttgccaa ttcgttcgtg
     3721 atataacaaa agatgatccg tatatgattg gccgaatcat tgcacgtccg tacgtgggtg
     3781 aaccaggaga cttcacacga acaagcaatc gccatgatta tgcgttgaat ccattcggac
     3841 acacagtcct tgattctttg aaagaagcag gaaaggacgt gattgctgtt ggaaagatca
     3901 acgatatttt caacggacaa ggaatcactg aatctgtccg cacgaaaagc aatatggatg
     3961 gcgttgacca gctgctgaaa gttatgaaaa acgatttttc aggaatcagc tttaccaatt
     4021 tagtagattt tgatgcatta tacggtcatc gtcgggatgt agtcggctat gcgcatgcga
     4081 ttgaagaatt tgacttacga attccagaat tgcttgaagc gatggaagaa gacgacttgc
     4141 taatgattac agccgatcac ggaaacgatc cgacgtttac tggtaccgat catacgcgag
     4201 aatatgtacc gttattggtt tacagtaaaa aaatgaaaga acaaggggca ttgcctcaag
     4261 gatattattc agatatttca gcaacgattg ctgagaactt tagtgtttct tcgactgaaa
     4321 atggcgaaag cttcttgaaa ttattaaaat aatcttttta aataggagaa agcaaatgac
     4381 caagttaagc gagatgttac aagaaacgac ccagtttatc cttgataaag gagtaaaaga
     4441 aattgatttt ggtttgattc taggttcagg attaggtgaa ttagcagatg aaatcgaaga
     4501 tgctgtaatg atttcttatg atgagatccc gttctttcct gtttcgacag tagttgggca
     4561 tgcaggaaag ctagtttatg ggtctctggc aggcaagaaa gtgttagcca tgcaagggag
     4621 attccactac tatgaaggtc attccatgca aacagtgacc tatccagtac gtgtgatggc
     4681 cggactaggg gcccattcag tcattgtgac taatgcatgt ggtggtgtca acgaatcatt
     4741 tgtaccagga gatttgatgc tgattaccga tcatatcaat tttactggtg aaaatccttt
     4801 gatcggtccg aatgaagatg caatgggccc tagatttcca gatatgagtc aagcttatac
     4861 gcctgactat caagcaacag caaaagcagc agcagaaaaa ctaggtttgg atcttaaaga
     4921 aggcgtatat atgggctatt ctggaccaac ttatgaaacg cctgcggaga tccgaatgtc
     4981 tcggacaata ggagccgatg cagtagggat gtcgactgtt ccagaagtca tcgtagcagc
     5041 tcacagcgga ttaaaagtgt taggaatctc ttgtattacc aacttagcag caggtatgca
     5101 agcgaatctg agccatgaag aagttgtaga aacaactcag agagtcaaac aatcttttaa
     5161 agcattggtt aaagaaacat tgacattatt gtaataggag gaacaataaa atgagtgtgc
     5221 atattgaagc gaaagaaggc gagatcgccg ataagatttt acttccggga gatcctttgc
     5281 gagctaaata tattgcggag acttttttgg aagatccagt ttgttacaat caggtacgcg
     5341 gtatgttagg ctttaccggc aaatataaag gagaacgtat ttctgttcaa ggaacaggaa
     5401 tgggaatgcc atcagccacc atctatgccc atgaattgat ccagtcgtat ggtgtaaaga
     5461 aattgattcg agtaggtaca tgcggtgcat tgtcaaaaga cgtccatgtt cgtgatttag
     5521 tactagcaca aggtgcagcg accagttctt cgatgatcga aaaaaatttc caagcctttc
     5581 attttcctcc aatcagtgat ttcaatctat tactgaaagc atatgagata gctaaagaaa
     5641 agggctatac ggttcatgta ggaaatgttc tttcggaaga ttctttctac aaagatgatt
     5701 tgacagagac gttccaatta gctgaacttg gcgttttagg agtggaaatg gaagcagcgg
     5761 ctctttatta cttaggcgcg aaataccatg tgcagacatt aagtttgatg accgttagcg
     5821 accatttgat caccggtgaa gaaacaacag ctgcagaacg tcaatctacg ttcaatgaaa
     5881 tgatcgaagt aggattggaa acagcgatag cagaataaga aggaaatgaa aaagctgccc
     5941 tgcattaagt aataagaaca tccaagcaaa aatagctcct catgtttttg acttggctgg
     6001 acttattatt gcaaatgtaa gcttttgaac accgtttacc aagaggttgt gaa
//
LOCUS       DACEEZ010000126         5841 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20313_2017
            SAMN12639966-rid9879223.denovo.129, whole genome shotgun sequence.
ACCESSION   DACEEZ010000126 DACEEZ010000000
VERSION     DACEEZ010000126.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639966
            Sequence Read Archive: SRR10023158
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5841)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5841)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741683.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741683.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:44:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,016
            CDSs (total)                      :: 2,962
            Genes (coding)                    :: 2,889
            CDSs (with protein)               :: 2,889
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 36 of 73
            Pseudo Genes (incomplete)         :: 29 of 73
            Pseudo Genes (internal stop)      :: 18 of 73
            Pseudo Genes (multiple problems)  :: 8 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5841
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639966-rid9879223.denovo.129"
                     /isolate="E20313_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            42..1472
                     /locus_tag="IWO89_002668"
     CDS             42..1472
                     /locus_tag="IWO89_002668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001878362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetM/TetW/TetO/TetS family tetracycline
                     resistance ribosomal protection protein"
                     /protein_id="HAQ0457364.1"
                     /translation="MCVMSCTEPEQWDVVIEGNDDLLEKYMSGKSLEALELEQEEIRR
                     FQNCSLYPVYHGSAKSNIGIEQLIEVITNKFYSSTYRKKSELCGNVFKIEYSEERQRL
                     AYVRLYSGVLHLRDSVRISEKEKIKITEMYTSINGELCKIDKAYSGEIVILQNEFLKL
                     NSVLGDTKLLPQRERIENPLPLLQTTVEPSKPQQREMLLDALLEISDSDPLLQYYVDS
                     TTHEIILSFLGKVQMEVTCALLQEKYHVEVKIKKPTVIYMERPLKKAEYTIHIEVPPN
                     PFWASIGLSVEPLPLGSGVQYESSVSLGYLNQSFQNAVMEGIRYGCEQGLYGWNVTDC
                     KICFKYGLYYSPVSTPADFRMLAPIVLEQVLKKAGTELLEPYLSFKIYAPQEYLSRAY
                     NDAPKYCANIVDTQLKNNEVILSGEIPARCIQEYRSDLTFFTNGRSVCLTELKGYHVT
                     TGEPVCQPRRPNSRIDKVRYMFNKIT"
     gene            1582..1632
                     /locus_tag="IWO89_002669"
                     /pseudo
     CDS             1582..1632
                     /locus_tag="IWO89_002669"
                     /inference="COORDINATES: protein motif:HMM:NF019662.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance efflux system leader
                     peptide"
     gene            1666..3042
                     /gene="tet(L)"
                     /locus_tag="IWO89_002670"
     CDS             1666..3042
                     /gene="tet(L)"
                     /locus_tag="IWO89_002670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001574277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline efflux MFS transporter Tet(L)"
                     /protein_id="HAQ0457365.1"
                     /translation="MNTSYSQSNLRHNQILIWLCILSFFSVLNEMVLNVSLPDIANDF
                     NKSPASTNWVNTAFMLTFSIGTAVYGKLSDQLGIKRLLLFGIIINCFGALIGFVGHSF
                     FSLLIMARFIQGAGAAAFPALVMVVVARYIPKENRGKAFGLIGSIVAMGEGVGPAIGG
                     MIAHYIHWSYLLLIPTITIITVPFLMKLLKKEVRIKGHFDIKGIILMSVGIVFFMLFT
                     TSYSISFLIVSVLSFLIFVKHIRKVTDPFVDPGLGKNIPFMIGVLCGGIIFGTVAGFV
                     SMVPYMMKDVYQLSTAEIGSVIIFPGTMSVIIFGYIGGILVDRRGPLYVLNIGVTFLS
                     VSFLTASFLLETTSWFMTIIMVFVLGGLSFTKTVISTIVSSSLKQQEAGAGMSLLNFT
                     SFLSEGTGIAIVGGLLSIPLLDQRLLPMEVDQSTYLYSNLLLLFSGIIVISWLVTLNV
                     YKHSQRDF"
     gene            3606..4868
                     /locus_tag="IWO89_002671"
     CDS             3606..4868
                     /locus_tag="IWO89_002671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001619005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Plasmid recombination enzyme type 2"
                     /protein_id="HAQ0457366.1"
                     /translation="MSYAVCRMQKVKSAGLKGMQFHNQRERKSRTNDDIDHERTRENY
                     DLKNDKNIDYNERVKEIIESQKTGTRKTRKDAVLVNELLVTSDRDFFEQLDPGEQKRF
                     FEESYKLFSERYGKQNIAYATVHNDEQTPHMHLGVVPMRDGKLQGKNVFNRQELLWLQ
                     DKFPEHMKKQGFELKRGERGSDRKHIETAKFKKQTLEKEIDFLEKNLAVKKDEWTAYS
                     DKVKSDLEVPAKRHMKSVEVPTGEKSMFGLGKEIMKTEKKPTKNVVISERDYKNLVTA
                     ARDNDRLKQHVRNLMSTDMAREYKKLSKEHGQVKEKYSGLVERFNENVNDYNELLEEN
                     KSLKSKISDLKRDVSLIYESTKEFLKERTDGLKAFKNVFKGFVDKVKDKTAQFQEKHD
                     LEPKKNEFELTHNREVKKERSRDQGMSL"
     gene            5113..>5841
                     /locus_tag="IWO89_002672"
     CDS             5113..>5841
                     /locus_tag="IWO89_002672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001611915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein rep"
                     /protein_id="HAQ0457367.1"
                     /translation="MCPNSSIYSDEKSRVLVDKTKSGKVRPWREKKIANVDYFELLHI
                     LEFKKAERVKDCAEILEYKQNRETGERKLYRVWFCKSRLCPMCNWRRAMKHGIQSQKV
                     VAEVIKQKPTVRWLFLTLTVKNVYDGEELNKSLSDMAQGFRRMMQYKKINKNLVGFMR
                     ATEVTINNKDNSYNQHMHVLVCVEPTYFKNTENYVNQKQWIQFWKKAMKLDYDPNVKV
                     QMIRPKNKYKSDIQSAIDETAKYPV"
BASE COUNT         2017 a          838 c         1201 g         1785 t
ORIGIN      
        1 tggaaattat aatcaaacag aaagtagagc tgcaccctaa tatgtgtgtg atgagctgta
       61 cggaacctga gcaatgggat gtggtaatag aaggaaatga tgacctttta gagaaatata
      121 tgtccggtaa atcattagaa gcattagaac tcgaacaaga ggaaatcaga agatttcaga
      181 attgctcctt gtaccctgtt tatcatggaa gcgcaaaaag caacataggg attgagcagc
      241 ttatagaagt gataacgaat aaattttatt catcaacata cagaaagaag tctgaacttt
      301 gcggaaatgt cttcaaaatt gaatattcgg aagaaagaca acgtcttgca tatgtacgcc
      361 tttatagtgg agtactacat ttgcgggatt cggttagaat atcggaaaag gaaaaaataa
      421 aaattacaga aatgtatact tcaataaatg gtgaattatg taaaattgat aaggcttatt
      481 ccggggaaat tgttattttg caaaatgagt ttttgaagct aaatagtgtt cttggagata
      541 caaagctatt gccacagaga gagagaattg aaaatccgct ccctctgctg caaacaactg
      601 ttgaaccgag caaacctcaa caaagggaaa tgttacttga tgcactttta gaaatctccg
      661 acagtgaccc gcttctacaa tattatgtgg attctacgac acatgaaatc atactttctt
      721 tcttagggaa agtacaaatg gaagtgactt gtgctctatt gcaagaaaag tatcatgtgg
      781 aggtaaaaat aaaaaagcct acagtcattt atatggaaag accgttaaaa aaagcagagt
      841 ataccattca catcgaagtg ccaccgaatc ccttctgggc ttccattggt ctttctgtag
      901 aaccgcttcc attagggagc ggagtacagt atgagagctc ggtttctctt ggatacttaa
      961 atcaatcgtt tcaaaatgca gttatggaag ggatacgata tggctgtgaa caaggattgt
     1021 atggttggaa tgtgacggac tgtaaaatct gttttaagta tggcttatac tatagccctg
     1081 ttagtacccc agcagatttt cggatgcttg ctcctattgt attggaacaa gtcttaaaaa
     1141 aagctggaac agaattgtta gagccatatc ttagttttaa aatttatgcg ccacaggaat
     1201 atctttcacg agcatacaac gatgctccta aatattgtgc gaacatcgta gacactcaat
     1261 tgaaaaataa tgaggtcatt cttagtggag aaatccctgc tcggtgtatt caagaatatc
     1321 gtagtgattt aactttcttt acaaatggac gtagtgtttg tttaacagag ttaaaagggt
     1381 accatgttac taccggtgaa cctgtttgcc agccccgtcg tccaaatagt cggatagata
     1441 aagtacgata tatgttcaat aaaataactt agtgtatttt atgttgttat ataaatatgg
     1501 tttcttgtta aataagatga aatatttttt aataaagatt tgaattaaag tgtaaaggag
     1561 gaattaatta tgaagtgaaa tgaatgtaac agggttcaat taaaagaggg aagcgtatca
     1621 ttaaccctat aaactacgtc tgccctcatt attggagggt gaaatgtgaa tacatcctat
     1681 tcacaatcga atttacgaca caaccaaatt ttaatttggc tttgcatttt atcttttttt
     1741 agcgtattaa atgaaatggt tttgaacgtc tcattacctg atattgcaaa tgattttaat
     1801 aaatcacctg cgagtacaaa ctgggtgaac acagccttta tgttaacctt ttccattgga
     1861 acagctgtat atggaaagct atctgatcaa ttaggcatca aaaggttact cctatttgga
     1921 attataataa attgtttcgg ggcgttaatt gggtttgttg gccattcttt cttttcctta
     1981 cttattatgg ctcgttttat tcaaggggct ggtgcagctg catttccagc actcgtaatg
     2041 gttgtagttg cgcgctatat tccaaaggaa aataggggta aagcatttgg tcttattgga
     2101 tcgatagtag ccatgggaga aggagtcggt ccagcgattg gtggaatgat agcccattat
     2161 attcattggt cctatcttct actcattcct acgataacaa ttatcactgt tccgtttctt
     2221 atgaaattat taaagaaaga agtaaggata aaaggtcatt ttgatatcaa aggaattata
     2281 ctaatgtctg taggcattgt attttttatg ttgtttacaa catcatatag catttctttt
     2341 cttatcgtta gcgtgctgtc attcctgata tttgtaaaac atatcaggaa agtaacagat
     2401 ccttttgttg atcccggatt agggaaaaat atacctttta tgattggagt tctttgtggg
     2461 ggaattatat ttggaacagt agcagggttt gtctctatgg ttccttatat gatgaaagat
     2521 gtttaccagc taagtactgc cgaaatcgga agtgtaatta ttttccctgg aacaatgagt
     2581 gtcattattt tcggctacat tggtgggata cttgttgata gaagaggtcc tttatacgtg
     2641 ttaaacatcg gagttacatt tctttctgtt agctttttaa ctgcttcctt tcttttagaa
     2701 acaacatcat ggttcatgac aattataatg gtatttgttt taggtgggct ttcgttcacc
     2761 aaaacagtta tatcaacaat tgtttcaagt agcttgaaac agcaggaagc tggtgctgga
     2821 atgagtttgc ttaactttac cagcttttta tcagagggaa caggtattgc aattgtaggt
     2881 ggtttattat ccataccctt acttgatcaa aggttgttac ctatggaagt tgatcagtca
     2941 acttatctgt atagtaattt gttattactt ttttcaggaa tcattgtcat tagttggctg
     3001 gttaccttga atgtatataa acattctcaa agggatttct aaatcgttaa gggatcaact
     3061 ttgggagaga gttcaaaatt gatccttttt ttataacagg aattcaaatc tttttgttcc
     3121 attaaagggc gcgattgctg aataaaagat acgagagacc tctcttgtat cttttttatt
     3181 ttgagtggtt ttgtccgtta cactagaaaa ccgaaagaca ataaaaattt tattcttgct
     3241 gagtctggct ttcggtaagc tagacaaaac ggacaaaata aaaattggca agggtttaaa
     3301 ggtggagatt ttttgagtga tcttctcaaa aaatactacc tgtcccttgc tgatttttaa
     3361 acgagcacga gagcaaaacc cccctttgct gaggtggcag agggcaggtt tttttgtttc
     3421 ttttttctcg taaaaaaaag aaaggtctta aaggttttat ggttttggtc ggcactgccg
     3481 acagcctcgc agagcacaca ctttatgaat ataaagtata gtgtgttata ctttacttgg
     3541 aagtggttgc cggaaagagc gaaaatgcct cacatttgtg ccacctaaaa aggagcgatt
     3601 tacatatgag ttatgcagtt tgtagaatgc aaaaagtgaa atcagctgga ctaaaaggca
     3661 tgcaatttca taatcaaaga gagcgaaaaa gtagaacgaa tgatgatatt gaccatgagc
     3721 gaacacgtga aaattatgat ttgaaaaatg ataaaaatat tgattacaac gaacgtgtca
     3781 aagaaattat tgaatcacaa aaaacaggta caagaaaaac gaggaaagat gctgttcttg
     3841 taaatgagtt gctagtaaca tctgaccgag atttttttga gcaactggat ccaggagaac
     3901 aaaaacgatt ttttgaggaa agttataaat tattttccga acgatatggc aagcaaaata
     3961 ttgcttatgc aacagttcat aatgatgagc aaacccctca catgcattta ggtgttgtgc
     4021 ctatgcgtga tggaaaactg caaggaaaaa atgtgtttaa tcgtcaagaa ctgttatggc
     4081 tacaagataa attccccgag catatgaaaa aacagggttt tgagttgaag cgtggtgaac
     4141 gtggctctga ccgtaaacat attgagacag ctaaatttaa aaaacaaact ttggaaaaag
     4201 agattgattt tctagaaaaa aatttagcag ttaaaaaaga tgaatggact gcttatagcg
     4261 ataaagttaa atcagattta gaagtaccag cgaaacgaca catgaaaagt gttgaagtgc
     4321 caacgggtga aaagtccatg tttggtttgg gaaaagaaat aatgaaaaca gaaaagaaac
     4381 caaccaaaaa tgttgttata tcggagcgtg attataaaaa cttagtgact gctgcgagag
     4441 ataacgatag gttaaaacag catgttagaa atctcatgag tactgatatg gcgagagaat
     4501 ataaaaaatt aagtaaagaa catgggcaag ttaaagaaaa atatagtggt cttgtagagc
     4561 gatttaatga aaatgtaaat gattataatg agttgcttga agaaaacaag tctttaaagt
     4621 ctaaaataag cgatttaaag cgtgatgtga gtttaatcta tgaaagcact aaggaattcc
     4681 ttaaggaacg tacagacggc ttaaaagcct ttaaaaacgt ttttaagggg tttgtagaca
     4741 aggtaaagga taaaacagca caattccaag aaaaacacga tttagaacct aaaaagaacg
     4801 aatttgaact aactcataac cgagaggtaa aaaaagaacg aagtcgagat cagggaatga
     4861 gtttataaaa taaaaaaagc acctgaaaag gtgtcttttt ttgatggttt tgaacttgtt
     4921 ctttcttatc ttgatacata tagaaataac gtcattttta ttttagttgc tgaaaggtgc
     4981 gttgaagtgt tggtatgtat gtgttttaaa gtattgaaaa cccttaaaat tggttgcaca
     5041 gaaaaacccc atctgttaaa gttataagtg accaaacaaa taactaaata gatgggggtt
     5101 tcttttaata ttatgtgtcc taatagtagc atttattcag atgaaaaatc aagggtttta
     5161 gtggacaaga caaaaagtgg aaaagtgaga ccatggagag aaaagaaaat cgctaatgtt
     5221 gattactttg aacttctgca tattcttgaa tttaaaaagg ctgaaagagt aaaagattgt
     5281 gctgaaatat tagagtataa acaaaatcgt gaaacaggcg aaagaaagtt gtatcgagtg
     5341 tggttttgta aatccaggct ttgtccaatg tgcaactgga ggagagcaat gaaacatggc
     5401 attcagtcac aaaaggttgt tgctgaagtt attaaacaaa agccaacagt tcgttggttg
     5461 tttctcacat taacagttaa aaatgtttat gatggcgaag aattaaataa gagtttgtca
     5521 gatatggctc aaggatttcg ccgaatgatg caatataaaa aaattaataa aaatcttgtt
     5581 ggttttatgc gtgcaacgga agtgacaata aataataaag ataattctta taatcagcac
     5641 atgcatgtat tggtatgtgt ggaaccaact tattttaaga atacagaaaa ctacgtgaat
     5701 caaaaacaat ggattcaatt ttggaaaaag gcaatgaaat tagactatga tccaaatgta
     5761 aaagttcaaa tgattcgacc gaaaaataaa tataaatcgg atatacaatc ggcaattgac
     5821 gaaactgcaa aatatcctgt a
//
LOCUS       DACEEZ010000127         5809 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20313_2017
            SAMN12639966-rid9879223.denovo.130, whole genome shotgun sequence.
ACCESSION   DACEEZ010000127 DACEEZ010000000
VERSION     DACEEZ010000127.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639966
            Sequence Read Archive: SRR10023158
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5809)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5809)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741683.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741683.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:44:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,016
            CDSs (total)                      :: 2,962
            Genes (coding)                    :: 2,889
            CDSs (with protein)               :: 2,889
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 36 of 73
            Pseudo Genes (incomplete)         :: 29 of 73
            Pseudo Genes (internal stop)      :: 18 of 73
            Pseudo Genes (multiple problems)  :: 8 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5809
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639966-rid9879223.denovo.130"
                     /isolate="E20313_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            450..1604
                     /locus_tag="IWO89_002673"
     CDS             450..1604
                     /locus_tag="IWO89_002673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase C47"
                     /protein_id="HAQ0457368.1"
                     /translation="MIILKPKNKIYLGLLFSLMIGIFSIGITNTYASENSNFNINSQP
                     IEEQAINFAKTDFEHSITSILLMENNTETDINYTLGSPFKMLDGENTANSSIFFPVFS
                     DADNTIKYVYTVKKNTNGEYSGSISQFLAKELQNLVDSNITDTTLFFQDNSIYYSDNG
                     QIKLLWKSPLSTEKNGVSSFTEKNDLISVNIKNKIKNFSKKQNFPAVKRVKSVSSGDN
                     FIMINWRIAETQTSFPWCAAYVEAAILCNKTDVRATSARAIMHYTYPNLNNSQIESKS
                     LNQDQIIKYANYCGVYPKKVARILNLSEVQTQLRKGNAIYMGTAGYGNYSNSRHALAL
                     FGWVNQGGYQTYYVWNPWYNYPTVISANAGPVTIPVPGGGFTWDNTITNW"
     gene            1598..2020
                     /locus_tag="IWO89_002674"
     CDS             1598..2020
                     /locus_tag="IWO89_002674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0457369.1"
                     /translation="MVNKPIVLVMGLVLVLFLTSCSKKQEIQKTELIPFLGTWQTKDH
                     SERLVITNLNTSKNIEQYTILVKENNHKIQIIDKNLNINEQVFIYKATNNLLTLRSNK
                     KQLDYTFSLDSPKKLVFILTTNDGTEGTAKPIEFFKAD"
     gene            3143..4075
                     /locus_tag="IWO89_002675"
     CDS             3143..4075
                     /locus_tag="IWO89_002675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HAQ0457370.1"
                     /translation="MANEIVKYKPELNTIPLRKFTPIEMNLFFSIVSRMRDKGNQKIR
                     FYFDQLKDLSAYKQTAGEVFIKDLTRTYDHLMDLRFGKKSNGGLTIERFTMFTDFEIH
                     GEADNPYVDIKVHEKALPLLNNLESWVRFSLTEFRDLNSSYAKTMFRLLKQYRTQGWA
                     EFSKEDFLELLDIPKSYRQTNINQFVLKPIKEELTPLFKGLTIRKKYGKGRGKPVIGY
                     RFTWKAEINHADDFSKGKQEDLRTKLFNIEHNGELTQEEKWRAKDRILDLPLGTHEAN
                     FKKQQQTEKEEVEKQTLSNELKQDLLGNLQNLFD"
     gene            4092..4607
                     /locus_tag="IWO89_002676"
     CDS             4092..4607
                     /locus_tag="IWO89_002676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF536 domain-containing protein"
                     /protein_id="HAQ0457371.1"
                     /translation="MADKTIKDLAEELGVSKTAINKKVSDENRKLWFAKIGNKFVINE
                     IGQKAIKSMFFTENENSNQQPVGEKSKTENHKNANQVSELELFFFLEKQLNQKDIQLQ
                     EKDEQLKQMQKLLDQQQQLTLQANQQIKKLHEQLQLTYAEESSEKEPLSVTEETQKGQ
                     NKEKKWWQFWQ"
     gene            4903..5184
                     /locus_tag="IWO89_002677"
     CDS             4903..5184
                     /locus_tag="IWO89_002677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0457372.1"
                     /translation="MDILVRNINPKIITRVDKRCQELTTKTGEKWSRNKYLKVLIESD
                     FDRQLMNYKKEQFDLLLEKFIDIQTKNTETLNDYVHTNNQLIETLIGGI"
     gene            5186..5602
                     /locus_tag="IWO89_002678"
     CDS             5186..5602
                     /locus_tag="IWO89_002678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001232859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0457373.1"
                     /translation="MRKTSVQKHVTLYPSHIDVLDSIIEKNPDINNYSEAIRFLCMSY
                     EPDTNTTTTSNQAKLNAMGKEISIMTEIIAEFADLYLKDTGVLIGTNSKVYQEVKEKI
                     EKQLNPSQRNQPKNRQKETSQTFTSKNFDPTNFLKR"
BASE COUNT         2228 a          961 c          930 g         1690 t
ORIGIN      
        1 aaaaaaaaag actgctcgaa agagcagtct caagcaaaaa ataaacgaac aaacgcaatt
       61 actcacgaac acaaacaaat acaataaaat acctaaataa tgaaaataag tgataaaaaa
      121 caagggtatt tatagtttaa caagcgctca taaaaataac aagtctttaa aaaatgacag
      181 ataaaaatgc acaaagaatc ggacacagat aaccgcgaaa aatcaagctt tagaaagttg
      241 tttttagcta gaccaatgaa aaatcacaaa gtccgcccct gtatccgttg gtagaggggc
      301 tacttggttt agtatacatt atgttaacta gctcgttaaa taacgattat gttcccaaaa
      361 caatatttta tgaactataa caaaaatttg ttatcgcata ttgtatattt taattatagg
      421 tgtatatcac ttaattttaa cacaaaggcg tgattatttt gaaacccaaa aataaaattt
      481 atctaggtct cctcttttcc ttgatgattg gaatttttag tataggaatt acaaatacct
      541 atgcttcaga aaattctaat ttcaatatca actcccaacc aattgaagaa caagcaatta
      601 attttgcgaa aactgatttt gaacactcca taacttcaat tcttttaatg gaaaataaca
      661 ctgaaactga tattaactat actcttggtt cgccatttaa aatgctcgat ggagaaaata
      721 cggcaaatag ttctattttc tttccagtat tttcagacgc tgataatacg attaaatatg
      781 tttataccgt caaaaaaaat acaaatggag aatattcagg aagtatttca caatttttag
      841 caaaagaact tcaaaatcta gtagatagca atataacaga tacaacctta ttttttcaag
      901 ataatagcat ttattattcc gataatggcc aaattaaact actttggaaa tcacctttaa
      961 gtacagaaaa aaatggtgtc tcttccttta cagaaaaaaa tgatcttata tccgtaaata
     1021 taaaaaataa aattaaaaat ttctctaaaa aacaaaattt tcctgcggtt aaacgtgtaa
     1081 aaagtgtctc ctccggagat aactttatta tgattaattg gcgtatagca gaaacccaaa
     1141 caagttttcc gtggtgcgct gcttacgtag aagctgctat cttatgtaat aaaacagatg
     1201 tacgcgctac ttcagctaga gccattatgc attataccta tcctaattta aacaacagtc
     1261 aaattgaatc aaaaagtctt aatcaagatc aaataataaa atatgcaaat tactgtggtg
     1321 tttatcctaa aaaagtagca cgaatactaa acctttcaga ggtccaaaca caactaagaa
     1381 aaggaaatgc catttatatg ggaactgctg gttatggaaa ttatagtaac agccgtcatg
     1441 cacttgcatt atttgggtgg gtcaatcaag gtggttatca aacttactat gtgtggaatc
     1501 cttggtataa ctacccaact gtaataagtg caaatgcagg gccagttaca attcctgtac
     1561 ctggaggtgg ttttacttgg gacaatacca ttacaaattg gtaaataaac ccattgtttt
     1621 agttatgggt ctagtgttag ttcttttctt aactagttgt tcaaaaaaac aagaaataca
     1681 aaagactgag ctgattcctt ttctaggaac ttggcaaact aaagatcact cagaacgtct
     1741 agtaatcact aatttaaata cttcaaaaaa tatagaacaa tataccatct tagtaaaaga
     1801 gaacaatcat aaaattcaaa tcattgataa aaatttaaat ataaatgaac aagttttcat
     1861 ttacaaagca acaaataatt tactaacact gcgctctaat aaaaaacaat tagattacac
     1921 ttttagtttg gattctccaa aaaagctcgt atttattttg acaacgaatg atgggacaga
     1981 agggacagct aaaccaattg aattttttaa agctgattga tgaatagttg tttgatatta
     2041 caaaacaata atttcaccga aggtgaaaca agcgaaactt tacaaaaagt ggctagcatt
     2101 tgctagcctt ttttgtattg cggagcgtga gttttgaggg ggattcaagg gggagtttct
     2161 cccctttgac aagcggttat ttgcaccaaa tacgaaggta tttggctagc aaatatagag
     2221 cttgccaaac cctttagtgt gttttgtaga aagggacctt atagtcactt tcacattaca
     2281 aacgatacgt cctatggacg tatcaaaagg gtgggactta tgagcaaatt aaagcaaaat
     2341 ttggctagca atccttctaa aaaatacacc tattgatcag agtcaaaata aatcagtttt
     2401 cgagtgagcg aatccgaatt tgcaaatctg aagctttgca aatgagtgtg ccggcttccg
     2461 taaaagtcta ggtaacatta gtcgatatat acataaggct ctaatttgcg ttttaaggcg
     2521 ttttaaacgt tctggtatgt aatttatacc aaagcgactt taaaacgcct taaaacgaat
     2581 tttataggtc ttgtaggacg tttcagggtt gttagggctt gccctgaccg cctgtaaggc
     2641 gctttaaaaa caagataagg gattttatct agacttgtct taaaacgccg tagatgagcg
     2701 ctcagagctt cttaaaggac tccttacgaa ctgtttttaa gcaaaggagc ctttaggcga
     2761 ctgcgcagtt caatgtgaga gcgtagcgat cacaccttta aagcgaccgt agggagcaga
     2821 ttcccagttt aggaaatttc gcgaacctgt ttggagaaaa aaagtaggaa atttttaggc
     2881 gggaaatttt gcccccctct tttataatct cttttataac ctctttttaa acctctttac
     2941 cgtcggctgt atccttactc tcccaaggcc tgtagctttt ttagggcatg aaattatctg
     3001 tttataggca tgaaattatc tgtttatagg catgaaatta tctgtttata ggcatgaaat
     3061 tatctgtttt aggtattgtt ttaagacata atatacctta aaattatgtc aataaaatta
     3121 gttagactgg agtatcaaat aaatggcaaa tgaaatcgta aaatataaac cagaattaaa
     3181 taccattcct ttaaggaaat ttactccaat agaaatgaac ttgttttttt ctattgtttc
     3241 tagaatgcgt gataaaggta atcaaaaaat acgcttttat ttcgatcagc ttaaagattt
     3301 aagtgcttat aagcaaactg ctggagaagt ctttataaaa gatttaacaa gaacatacga
     3361 tcatttaatg gatttacgtt ttggaaaaaa aagcaatggt ggattaacca tagaacgatt
     3421 tacaatgttt actgattttg aaattcatgg agaagccgat aacccttatg tggatataaa
     3481 agttcacgaa aaagctttgc ctttacttaa caatttagaa agttgggtac gtttctcttt
     3541 aacagaattt cgagatttaa atagcagtta tgctaaaaca atgtttagat tactaaaaca
     3601 ataccgaaca caaggatggg ctgaattttc caaggaagat tttttagaac tattagatat
     3661 tcctaaaagc tatagacaaa caaatataaa tcagtttgta ctcaaaccca taaaagaaga
     3721 actcacacca ttatttaaag gattaacgat tcgtaaaaaa tatggtaaag gccgaggcaa
     3781 acctgttatt ggttatcgtt ttacttggaa agctgaaatt aatcatgctg acgatttttc
     3841 aaaagggaag caagaagatt tacgtacaaa attatttaat attgaacaca atggagaatt
     3901 aacgcaagaa gaaaaatggc gcgctaaaga tcgaatacta gatcttccgt taggtactca
     3961 tgaagctaat tttaaaaaac aacaacaaac tgaaaaagaa gaagtggaaa aacaaacact
     4021 ttcaaacgaa cttaaacaag atttattagg aaatttacaa aacttattcg attaaagtat
     4081 aaggagtatt tatggcagat aaaacgatca aagaccttgc tgaagaatta ggagttagta
     4141 agacagctat taacaaaaaa gtttctgacg aaaatcgaaa actttggttt gcgaaaatcg
     4201 gaaacaagtt tgtgattaac gaaatcggtc aaaaagctat aaaatcaatg ttttttaccg
     4261 aaaacgaaaa ctcaaatcaa caaccagttg gcgaaaaatc gaaaaccgaa aaccataaaa
     4321 acgccaacca agtttccgaa ttagaactct tttttttcct agaaaaacaa ttaaatcaaa
     4381 aagatataca gctacaagaa aaagacgaac aattaaaaca aatgcaaaaa ttactcgacc
     4441 agcaacaaca actcacctta caagccaatc aacaaatcaa aaaacttcac gaacaactcc
     4501 agctaaccta tgcagaagaa tcttcagaaa aagaacccct ttctgttact gaagaaactc
     4561 aaaaaggaca aaacaaggag aaaaaatggt ggcaattctg gcaataaccc aacaattttt
     4621 attcataaaa attgttgggt tactttcttt ttttgctttt acaagcatag cgacatgatc
     4681 tcacgtcgct attttttaaa gagcataagg aaacgcggta tcactttctt acgaaaggtg
     4741 ctaggtaggc tgtgcctacc acgtttctaa gaaaaaaatt tgctatcact ttttcgccat
     4801 tgtggctagc ttatgtatag tatttcggta aaaaacagct atacaaatac tatatttgta
     4861 tgatatacat aaattataca cacataagaa aggacgtttt ctatggacat tttagttcga
     4921 aatatcaatc caaaaattat tacaagagta gataaacgtt gtcaggaact tacaaccaaa
     4981 acaggagaaa aatggagtcg aaataaatat ttaaaagtgc taatcgaaag tgattttgac
     5041 cgtcaactta tgaattacaa aaaagagcaa ttcgatttat tactcgaaaa atttatcgat
     5101 attcaaacaa aaaatactga aacattgaac gactatgttc atacaaacaa ccaactcatt
     5161 gaaacactta tcggaggaat ataacatgag aaaaacttct gttcaaaaac acgttaccct
     5221 ttatcccagt cacattgacg ttttagattc catcatcgaa aaaaatccag atatcaataa
     5281 ctactcggaa gccattcgct ttttatgtat gagttatgaa cctgatacca atacaacaac
     5341 taccagtaac caagccaaac taaatgcgat gggaaaagaa atttctatca tgaccgaaat
     5401 tatcgcagaa tttgctgatt tgtatttaaa agatacaggc gttcttatcg gtacaaactc
     5461 gaaagtatat caagaagtaa aagaaaaaat tgaaaaacag ctaaatccct cccaacgaaa
     5521 tcaaccaaag aatcgtcaaa aagaaacgag tcaaacattc acttctaaaa atttcgaccc
     5581 aaccaacttt ctaaaaagat aaaaaaataa accaggaaat ttttaatttc ctggtttatt
     5641 ttctagagag cggtcttcca ataagatttg caaatcgatc ccaatttgcg taaaaaaaaa
     5701 gctgctcgta cgagcagcta aacaagaaaa agtcagcttg ttttaagggt aaaataagtg
     5761 taacaaattt tgcgaatgca acgatctttt ttattatacg caaacaatc
//
LOCUS       DACEEZ010000128         5790 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20313_2017
            SAMN12639966-rid9879223.denovo.131, whole genome shotgun sequence.
ACCESSION   DACEEZ010000128 DACEEZ010000000
VERSION     DACEEZ010000128.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639966
            Sequence Read Archive: SRR10023158
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5790)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5790)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741683.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741683.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:44:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,016
            CDSs (total)                      :: 2,962
            Genes (coding)                    :: 2,889
            CDSs (with protein)               :: 2,889
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 36 of 73
            Pseudo Genes (incomplete)         :: 29 of 73
            Pseudo Genes (internal stop)      :: 18 of 73
            Pseudo Genes (multiple problems)  :: 8 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5790
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639966-rid9879223.denovo.131"
                     /isolate="E20313_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(99..1229)
                     /locus_tag="IWO89_002679"
     CDS             complement(99..1229)
                     /locus_tag="IWO89_002679"
                     /EC_number="2.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-methyltetrahydropteroyltriglutamate--
                     homocysteine S-methyltransferase"
                     /protein_id="HAQ0457374.1"
                     /translation="MSKNRPFTVDHVGSFLRPQSIKEARKKVQEGLLSKEELRAIEDQ
                     EIKKLVEDQKAVGIKGITDGEFRRGFWHLDFLEELNGIEGYVPETGYNQTFHGKAAPA
                     YNIRVVDKLSFNPNHPFLKDFAFLKEVVETDDTFVAKATIPSPTMILRQEILSNDGSS
                     KIKEIYPDLSDFYEDLSSTYKAAIHAFYEKGCRYLQFDDTNWAFLADQTKREALLQKG
                     IDPKEMAHICTEIINNALEDKPEDLTITTHICRGNHASSWLFSGGYEPIAEELFSTNY
                     DGFFLEYDSDRAGDFTPLRHWSNKESKVVLGLVTSKFPDLEDTEKIKERIKEAQQFVP
                     LENLSISPQCGFASTEEGNHLTEEEQWKKIQLLQQVAKEVWL"
     gene            complement(1877..3079)
                     /locus_tag="IWO89_002680"
     CDS             complement(1877..3079)
                     /locus_tag="IWO89_002680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HAQ0457375.1"
                     /translation="MPKIKNIYQDKKTKKWFFRAYLGIDENGRKVQKTKRGYASQKDA
                     KIAYDKYMETHDFNKTIPNQLCSTNQMTFEEFYKDRFVKWYERQVKSQTYENAQFIFE
                     KRMQFFYHFRVRDITSHDIEDWLFELSQTESRNSRKKVNGETLSKSYINRIRGHLKIV
                     LDRAVKEGVIAKNPVEDVSSLPSENKKVEFWEHSEFQKVMNELKDDKIQTHHRKIVYE
                     MLFYTGIRIGELEALSWSNVDFEKNTITIEKTLIYNTKDDWYFSTPKTKCAYRTIGLG
                     KKLSLKLRQWYDLQKRIGNFEYVAQLDGTFTPPYSFANWLKEAAKKAGVKPIKLHALR
                     HSHVAFLIEQNIQPLSIQERLGHANIQITLGTYGHLYAKSDQQVVSAIDNVQEGAILS
                     KNDNLLPV"
     gene            complement(3139..3342)
                     /locus_tag="IWO89_002681"
     CDS             complement(3139..3342)
                     /locus_tag="IWO89_002681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3173 domain-containing protein"
                     /protein_id="HAQ0457376.1"
                     /translation="METKCDFMVNRAILIEMGFKPSQAARMIKESKAYLARIEGIDFY
                     NNRQVGVVPSRVIEHLFHIQVAE"
     gene            complement(3484..4716)
                     /locus_tag="IWO89_002682"
                     /pseudo
     CDS             complement(3484..4716)
                     /locus_tag="IWO89_002682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173388.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
     gene            4879..5691
                     /locus_tag="IWO89_002683"
     CDS             4879..5691
                     /locus_tag="IWO89_002683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002406512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAQ0457377.1"
                     /translation="MEIDKQAVGNRIRQIRQELKLSMEKFGKLIGDLPRSTVNNWERG
                     INLPKTETLHQIAEIGHVTNEYLLYGDQENQYILEMLQKKAGKLDPKIEGLIVDEIKH
                     ADLVSEKEMDRMIEFFITNLIPPTEQDQFTFQCIDEKKQLYLGSTNFGKEAQLYLQHD
                     NQNHILHMMPFTFSNFSVDRILVYLANQKSYSYFGKHLPKELGEKAILLYSINQLTSD
                     VRIAPLVYSEETASYQYTEDNQYLLEQQYLYLPFVMEVEKERLLNAAYPSSK"
BASE COUNT         1717 a         1189 c          921 g         1963 t
ORIGIN      
        1 aaataaataa tataaaaggt aaataaaaaa gagtggattt ctccactcca tgaaaagccg
       61 actttttggg acgctttttt ttgccaagcc tcacattttt atagccaaac ttcttttgcg
      121 acttgctgca ataattggat tttcttccat tgttcttctt ctgttaaatg gtttccttct
      181 tctgttgaag caaacccaca ttgaggactg atacttagat tttccaatgg tacaaattgt
      241 tgtgcttctt ttattcgttc ttttattttc tccgtatctt ccaaatctgg aaacttagaa
      301 gtaactaatc ctaaaacaac tttactttct ttgttactcc aatgtcttaa aggagtaaaa
      361 tctcctgctc ggtcagaatc gtattccaaa aagaaaccat cataatttgt tgaaaacaac
      421 tcttctgcaa tgggttcata tccaccggaa aacagccaag aagaagcgtg attcccgcgg
      481 cagatatgtg ttgtgatcgt caaatcttct ggtttgtctt ccagtgcgtt attgataatc
      541 tctgtacaaa tatgggccat ttctttcgga tcaattcctt tttgaagaag tgcttctctt
      601 ttagtttgat cagctagaaa cgcccaattt gtatcatcga attgcaggta acggcaacct
      661 ttttcataaa aagcatgaat cgctgctttg taagtagagg acaagtcctc ataaaaatca
      721 gatagatcag gataaatctc cttgattttt gaagaaccat cattagatag gatctcttgt
      781 cgtaaaatca tcgtgggact agggatcgtt gcttttgcaa caaaagtatc atctgtttcc
      841 actacttctt tcagaaaagc aaaatctttt aaaaatgggt gatttggatt gaaagataat
      901 ttatctacta cccgaatatt ataagctggt gctgcttttc cgtggaatgt ttgattatat
      961 ccagtttctg gaacatagcc ttcgatacca tttagttctt ctaaaaaatc aagatgccaa
     1021 aatccacgcc gaaattcacc atctgtaatt cctttgatgc cgactgcttt ttggtcttcc
     1081 actaattttt ttatttcctg gtcttcgatt gcacgaagtt cttcttttga aagtaagcct
     1141 tcttgaactt tttttcttgc ttctttgatc gattgcgggc gtaagaaact tccgacatga
     1201 tctactgtaa atggtctatt tttgctcatt tcactcactc ttttctttgt tttttgtaaa
     1261 aaaaatcctt ttactagcaa atactagtaa aaggacgatt atactcgtgg taccaccttt
     1321 tttcagaaaa aacattcttt ctctcagcct gacaataaaa tcagtggtct tggtaacgaa
     1381 agactgttcg tttttgccta cctttcgaca aaaaatcctc taaggccatt ttcgacagtc
     1441 aacttctagc tttttacacc aaccaaagcc tctcttgaac cattttctgt ctactttcct
     1501 tctcatcgga tactattaga ataaaattag aatagcatgt ttgaaagaaa agtcaatagg
     1561 gaataagaac aaacaaaaac taatttgtta ttcttagtaa tttgtttgtg gaatacaata
     1621 aagcttttct aatatgataa ttgttcagtc gtcctttcat cttagatcac ttccttaaaa
     1681 tgatcatacc cgtttcaacc acataaaaaa ggacttaatt ttgaaaaatt tcccatcaaa
     1741 actaagtcct ttttattata gttatgcaaa tttgcttatt ttctcgctat ttccgataca
     1801 tcttgaacag gagcaattcg actgaaaaca ctggtgcaaa agcatttatt tgtcttatgg
     1861 tagaaccgtg gaaaactcag actggtaata aattgtcatt ttttgaaaga atagcgccct
     1921 cttgaacatt atcaatcgca cttaccactt gttgatctga ctttgcatat aagtgaccgt
     1981 aagttcctaa agtgatttga atattcgcat ggcccaatcg ttcttgaata gatagcggtt
     2041 gaatattctg ctcaattaga aatgcaacat gagaatgtcg tagcgcgtga agcttaatcg
     2101 gtttgacacc cgctttttta gcagcttctt ttaaccagtt cgcaaaagaa taaggtggcg
     2161 taaatgttcc gtccaactga gcaacatatt caaaatttcc aatacgtttt tgcaagtcat
     2221 accactgtct gagttttaag gaaagcttct tccccaaccc aatcgttcga taagcacatt
     2281 tagttttggg agtcgaaaag taccaatcat cttttgtatt ataaatcaat gtcttctcaa
     2341 tcgttatcgt gtttttctca aaatctacat tcgaccaaga caaagcttca agttctccaa
     2401 tccgaatgcc agtataaaag agcatttcat acacaatctt ccgatggtgg gtttgaatct
     2461 tatcatcctt caattcattc atcacttttt gaaactcact gtgttcccaa aactcaactt
     2521 tcttattctc agaaggaagc gaagaaacat cttcaacggg atttttagca atcaccccct
     2581 ctttaacagc ccgatccaat acaattttta ggtgccctcg aatccgattg atataagatt
     2641 ttgataacgt ttcgccatta acttttttcc gtgaattacg tgactctgtc tggcttagtt
     2701 caaataacca atcctcaata tcgtgacttg taatatctcg aacacgaaaa tgatagaaaa
     2761 actgcatacg cttttcgaaa atgaattgcg cattctcata tgtttgactt ttgacctgtc
     2821 tctcgtacca ttttacaaac ctatccttgt aaaactcttc gaaagtcatt tgattggtag
     2881 aacacagttg attaggaatt gtcttgttaa aatcatgtgt ttccatgtac ttgtcataag
     2941 caattttggc atctttttgt gaagcataac ctcgtttagt cttctgaacc tttcttccat
     3001 tttcatctat acctagatac gctcggaaga accatttttt tgtcttttta tcttggtaaa
     3061 tatttttaat ttttggcatc gttccattcc tccctgtact tgagaaaagc tactttaata
     3121 gtaccgcgct tgtactgttt attccgcaac ttgaatgtga aaaagatgtt caattactcg
     3181 ggatggcacc acaccaactt ggcgattatt ataaaaatca ataccttcaa tccttgcaag
     3241 atatgccttg ctctctttaa tcattcgtgc agcttgacta ggtttaaagc ccatctctat
     3301 caaaattgca cgatttacca tgaaatcaca ttttgtttcc attctctcac ctcctgattc
     3361 tttactcctt ttaaaataat cctctttata atctattgtt tctcattcga tggttgtgtt
     3421 atactcgtct tgtcgaggcg gagtacgttg cacaacttgt tgtgtaagca gtactttgct
     3481 tttttagtag cttgttttct catttgatat actttgtaat tttcgttcat aatactcaac
     3541 ctcctcctga taatactccg ggtctgcctt catattttgg agaagtttga tctgtcttgg
     3601 attcatttct ccttgttcaa taatcaaagg caacaaggaa gaatgaaacg tttgatcgac
     3661 ctgtgcaata aaaactaatg atggtgaaac accatgaatt aaccaactca tagttttctc
     3721 aaaactttga ggaattgtat ctaaagaaat ggtgattgcg ccataattgc taataaaggc
     3781 tgcccatttg gcatcaactt ttgcacctag atcatttgga tcacggtcat aaaaacaaat
     3841 tgctccatta agtaactcaa agaacagttt ggttaaatca tgggtttttg aaataatctt
     3901 tgccaagctt tgcgctttct cttttcgata ccgtagttca aagcgattct ttacttttgc
     3961 ctcttctaaa ggaattcctc gtcgcttccg ttgttcatgg tccttctgat aaaagcaaaa
     4021 acgacactga ctcttctttg agcctaaata caaggtctca ccatttgatt cattgtttat
     4081 gatatccgta ccgccatgag attcactcac ttgaaaagtg gtccacacat ggcctagtat
     4141 taccttatgc ttaagttctg gaatgctgag actccccaca aaatcatcta aagtaaaatc
     4201 aatccgtgta aagttcccct gataatctaa taccttacga aaaaactgtt gccaatttgt
     4261 ttttcgtgtt tttagccatc ttgctaagtg tttacagcct tgacctgaaa actccatcac
     4321 agttccttta accgcatcgt caatggaata ccgaatcgtg attacttgat tccacacgta
     4381 ttggccaata tagccctttg gcgcataatc cagttcctca aaacgttcaa tgggcatttc
     4441 aataatctct tcaattagct ttaaatgttg attcgagaaa aagtgaatgg ttagataatc
     4501 aatcaaaaca cggttaactg gaacgacctc ttcttgcaaa ctatccaaca caagattcaa
     4561 tttttcttca cttaattgtc tcgttccttt ttctactttc gataataact atctcgaaat
     4621 ccccactttt tctgctaaat atcgttgtgt ccaccccctt tcttctcgag aaactcgaat
     4681 ggcttcaccg agatcatgtt gattaacttg gttcatcgac aaacctcctt ctgctattta
     4741 cttgtcaaag aacctattga acttatttta agtacatttt tattgtacgc ctgtatttat
     4801 tttaagtcaa tatgttttga ctttttttaa gtacacacgt atactaaatt taagaactta
     4861 tcctgaggag gagttttcat ggagatagac aagcaagcag tcggcaatcg aattcggcag
     4921 attcgccaag aattaaagtt gagtatggag aaatttggca aactaattgg tgatttaccg
     4981 cgaagtacag tcaataactg ggaacgtggg atcaaccttc caaaaaccga aacccttcac
     5041 caaatcgcag agataggtca tgtcaccaat gaatatcttt tgtatggtga tcaagagaat
     5101 caatacattt tagaaatgct ccaaaaaaag gcaggtaagc ttgatcctaa gattgaaggt
     5161 ctcatagtag acgaaatcaa acacgctgac cttgttagtg aaaaagaaat ggatcgaatg
     5221 attgaatttt ttattacaaa tctcattcca ccaactgaac aagaccagtt tactttccaa
     5281 tgtatcgatg aaaaaaagca gctttatctt ggttcaacta attttggtaa ggaagcccag
     5341 ctctatttgc aacatgataa tcaaaatcat attctgcaca tgatgccctt taccttctcc
     5401 aactttagtg tggatcgcat actcgtctat ttagcaaatc aaaaatccta ttcctatttt
     5461 gggaaacatt tacccaagga actaggtgag aaagccatcc ttctatactc aattaatcaa
     5521 ttgaccagcg atgtgcgaat tgccccgttg gtctattcag aagaaaccgc ttcttatcag
     5581 tacacagagg acaatcaata tctactagaa cagcaatatc tttatctacc attcgtgatg
     5641 gaagtcgaga aagaacgcct gttaaatgca gcgtatccat catcaaaatg aggtgacaaa
     5701 attgataagc acgtgacaaa gaaaatactg ctaggacact ccccaatggg tagttgtcac
     5761 agtttcctgt tctttgttag ccccctgtta
//
LOCUS       DACEEZ010000129         5785 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20313_2017
            SAMN12639966-rid9879223.denovo.132, whole genome shotgun sequence.
ACCESSION   DACEEZ010000129 DACEEZ010000000
VERSION     DACEEZ010000129.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639966
            Sequence Read Archive: SRR10023158
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5785)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5785)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741683.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741683.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:44:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,016
            CDSs (total)                      :: 2,962
            Genes (coding)                    :: 2,889
            CDSs (with protein)               :: 2,889
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 36 of 73
            Pseudo Genes (incomplete)         :: 29 of 73
            Pseudo Genes (internal stop)      :: 18 of 73
            Pseudo Genes (multiple problems)  :: 8 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5785
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639966-rid9879223.denovo.132"
                     /isolate="E20313_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(24..2195)
                     /gene="copB"
                     /locus_tag="IWO89_002684"
     CDS             complement(24..2195)
                     /gene="copB"
                     /locus_tag="IWO89_002684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper/silver-translocating P-type ATPase CopB"
                     /protein_id="HAQ0457378.1"
                     /translation="MNGNDMEKKHEHKKEVVNKESTESHEKQMEMKHDHSQMDHSMHM
                     GHNHGDIANSKQMDHSMHMDHEHGGMDHSMHMGNFKQKFWLSLILAIPIILFSPMMGM
                     EFPFQVTFPGSDWLVLILATILFIYGGQPFLSGAKMELKQKSPAMMTLIAMGITVAYI
                     YSVYSFIANLLNPHTHVMDFFWELATLIVIMLLGHWIEMNAVSNASNALQKLAELLPE
                     SVKRLTKDGKEETVSLKEVNEGDRLIVRSGDKMPTDGVILKGETIVDESAVTGESRGI
                     KKQANDKVIGGSINGDGTIEIEVTGTGENGYLAKVMEMVRKAQGEKSKLESLSDKVAK
                     WLFYVALIVGILAFIAWLFLTDLPNALERMVTVFIIACPHALGLAIPLVVARSTSIAA
                     KNGLLLKNRNALEQANDLDVIMLDKTGTLTEGKFTVTGVEVLDDAFNKNEILQYLGAL
                     EANANHPLAVGIMNYLKEHEIKPYQAENLKNLSGVGLEATVKKQQVKIVNEKEVERLG
                     LNVEQALLKPYQEQGNTISFLILENHLAAIVALGDVVKTEAKEFIRTLKERKITPVML
                     TGDNKNAAQAVADYLGIEEYYGGLLPDDKEAVVQKYLDQGKKVIMVGDGINDAPSLAR
                     ASIGMAIGAGTDIAIDSADVVLTNSDPKDILHFLDLAKQTRKKMIQNLWWGAGYNILA
                     IPLAAGILAPIGIILSPAVGAVLMSLSTVVVALNALTLKIR"
     gene            complement(2215..4401)
                     /locus_tag="IWO89_002685"
     CDS             complement(2215..4401)
                     /locus_tag="IWO89_002685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper-translocating P-type ATPase"
                     /protein_id="HAQ0457379.1"
                     /translation="MATAETKMDTFVITGMTCANCSARIEKELNGQPGVVNATVNLAT
                     EKASVKYEGTTTEKLIQSVENIGYGAILYDEAHKQKIAEEKQAYLKKMLFDLILSTVL
                     TLPLMLSMIAMMLGSHAAIVHFFHFPIVQLVLSAPVQFYVGARFYKGAYHAIKTKAPN
                     MDVLVAIGTSAAFALSIYNGFFRGHPQDLYFESSSMIITLILLGKYLEHTAKTKTGNA
                     IKQLMSLQTKTAQVIRNGKEETLAIEEVVVGDQLVIRPGEQIPADGRIISGSSAIDES
                     MLTGENLPVEKNPDDTLFGGTINTNGLLHMEVTQVGKQTVLAQIIQMVEDAQGSKAPI
                     QKIADRISGIFVPIVLVIAFITLIATGLITGDWQLALIHSVSVLVIACPCALGLATPT
                     AIMVGTGVGARNGILIKGGEALEAAAHLDSIVLDKTGTITEGKPKVTDLVGSKEVLSI
                     FYTLEQASEHPLGKAIVEYGKLQEAATYDMIDFTAHPGAGISGTINGVRYFAGTRKRL
                     IELNLSFDEYQEHALELEQQGKTVMFLADEKQVIGLIAVADQIKLEVKQAIKQLQNKG
                     LDVFMLTGDNKLAAETIGKQVGIDPKHIFAEVLPEDKAAYVEKLQKDGKKVGMAGDGI
                     NDAPALALADVGMAMGSGTDIAMETADVTLMNSSLASIAQTIELSRLTLRKIKQNLFW
                     AFVYNTIGIPFAALGFLNPIIAGGAMAFSSVSVLLNSLCLNRHMKK"
     gene            complement(4401..4610)
                     /gene="copZ"
                     /locus_tag="IWO89_002686"
     CDS             complement(4401..4610)
                     /gene="copZ"
                     /locus_tag="IWO89_002686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper chaperone CopZ"
                     /protein_id="HAQ0457380.1"
                     /translation="MKQQFSIEGMSCNHCAARVEETVSALDGVQKVKVNLKKANGTVK
                     FDETKVQSEKICQAINGLGYKAEVI"
     gene            complement(4623..5063)
                     /locus_tag="IWO89_002687"
     CDS             complement(4623..5063)
                     /locus_tag="IWO89_002687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopY/TcrY family copper transport repressor"
                     /protein_id="HAQ0457381.1"
                     /translation="MEKVKTPVKISDSEWEIMRVIWTLGQTTAQEITQILADSMDWKP
                     ATIKTLLGRLVKKEVIWTEQEGKKFIYYPAVSEMETVRSATENLFSHICAKRIGETIA
                     DLVEEATLTQEDINEILKQLEQKQPVDTIECNCIPGQCECKQHQ"
     gene            complement(5137..5676)
                     /locus_tag="IWO89_002688"
     CDS             complement(5137..5676)
                     /locus_tag="IWO89_002688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HAQ0457382.1"
                     /translation="MKIIVIGQSGSGKSTLARKIKEITDFPLLPLDLLWHTTDYSIQA
                     KKWFLQEQQLFMVSNPSWIVEGNYTSTLEERIKEADKIIWLKVPRYLAIYRVVWRSLK
                     RKINKKSRPDMPESFSEKLNREYLEFLSFIWHFEENNEPKIQQLIQVANARNKLIILK
                     TRREKQEFLTKLKIESKNN"
BASE COUNT         1495 a         1302 c         1009 g         1979 t
ORIGIN      
        1 tttgtcacaa ccttttttca ttattatctg attttcaatg tcaaagcatt tagagctaca
       61 actaccgtac ttaacgacat caagacagca ccaactgctg gacttaatat gataccaatt
      121 ggcgcaagaa ttccggctgc tagcggaatt gcaagaatat tgtaacctgc cccccaccag
      181 aggttctgga tcattttctt tcgcgtttgc ttggctagat caagaaaatg caaaatatct
      241 tttggatcgc tgtttgtcaa tacgacatcc gcagaatcga ttgcgatatc tgtccctgca
      301 ccgattgcca tcccgattga agcacgggct aaacttggcg catcattgat cccatcaccc
      361 accatgatta cttttttccc ttggtcaagg tatttctgga caacagcttc tttatcatct
      421 ggtaataaac cgccataata ttcttcgatc cctaaataat ctgctactgc ttgggcagcg
      481 tttttattgt caccagtaag catgacagga gtgatttttc tttctttcaa tgttcggata
      541 aattcttttg cttctgtttt tactacgtca cctaaagcaa cgattgcagc tagatgattt
      601 tctaaaatca ggaagctgat ggtatttcct tgttcttggt aaggtttcaa taatgcttgt
      661 tctacgttta atccgagacg ctcaacttct ttttcattaa ctattttcac ttgttgtttt
      721 ttgactgttg cctctagccc gactcctgaa agatttttta gattttcagc ttgataaggc
      781 ttgatttcat gttctttcaa ataattcata atgccaacag ccaatggatg attggcattc
      841 gcttccaagg ctcctagata ttgaaggatt tcatttttat tgaacgcatc atcaagtact
      901 tcgacacccg ttacagtaaa ttttccttct gttagtgttc ctgttttgtc cagcatgatc
      961 acatctagat cattggcttg ctccagtgca ttgcggtttt tcaaaagtaa tccgttttta
     1021 gctgctattg atgtggaacg agcaacaact aatgggatag ctaaacctag agcatgagga
     1081 caggcgatga taaagactgt taccatacgt tctaatgcat taggaagatc tgtcaagaac
     1141 aaccaggcga tgaatgccag gatacccaca attaatgcga cataaaacag ccattttgcc
     1201 actttgtcag ataatgactc tagcttggat ttttctcctt gtgcttttcg caccatttcc
     1261 ataaccttcg ctagataacc attttcacca gttccagtta cttcgatttc gatcgttcca
     1321 tccccattga tagaaccgcc aatcactttg tcattggctt gttttttgat tcctcttgat
     1381 tcgccagtca cagctgattc gtcaacaatt gtttcacctt tcaagattac accatctgtt
     1441 ggcattttat caccagaccg gacgatcaaa cgatcacctt cgtttacttc tttcaatgaa
     1501 actgtctctt cttttccatc tttcgtcaag cgtttcactg attctggcag taattctgct
     1561 agtttttgta aagcattgct ggcattcgac acagcattca tttcaatcca gtgacctagt
     1621 agcataataa cgatcaaagt agccaattcc cagaagaaat ccatgacgtg tgtatgtgga
     1681 ttgagcagat ttgcgataaa cgaatagaca ctataaatat atgcaactgt aatccccatc
     1741 gcgatcagtg tcatcatagc aggacttttt tgtttcaatt ccatcttagc tccacttaaa
     1801 aatggctgac cgccatagat aaatagtata gttgctaaga tcaatacgag ccaatcagaa
     1861 ccaggaaacg ttacttgaaa tggaaattcc attcccatca ttggtgaaaa aagaatgatt
     1921 gggatagcta ggataagcga tagccaaaat ttttgtttga aattccccat gtgcatggaa
     1981 tgatccatgc cgccgtgttc gtgatccatg tgcatcgaat ggtccatttg tttgctgttt
     2041 gctatatctc catggttatg tcccatgtgc atggaatgat ccatctgact atggtcatgt
     2101 ttcatctcca tctgtttttc atgagattct gtactttctt tgtttactac ttctttttta
     2161 tgctcatgtt ttttctccat atcgttccca ttcattttta ctccatccct tctcttactt
     2221 tttcatgtgt cgatttaagc acagggagtt caacaataca ctgacggaac taaacgccat
     2281 cgctcctcct gcaatgattg gattcaaaaa gcctagtgcg gcaaaaggaa taccaatcgt
     2341 attatagaca aaagcccaga aaagattttg tttgattttt ctcaatgtca acctagacaa
     2401 ctcgattgtt tgtgcaatgg atgccaaact gctattcatc agcgtcacat cggctgtttc
     2461 catcgcaatg tccgttccac ttcccattgc cataccgaca tcagctaatg ctaaggccgg
     2521 tgcatcattg attccatcgc ctgccatacc gacttttttg ccatctttct gaagtttttc
     2581 tacgtaggct gctttatcct ctggaagtac ttcagcaaag atatgctttg gatcaatacc
     2641 aacttgttta ccaatcgttt cggctgccag tttgttgtct cctgtcagca taaaaacatc
     2701 taatccttta ttctggagct gcttgattgc ttgtttgact tctagtttga tttgatcagc
     2761 cacggcaatc aacccgatca cttgcttttc atcagctaaa aacatgactg tttttccctg
     2821 ctgttctaat tctaacgcgt gttcttggta ttcatcaaat gataggttca attcaattaa
     2881 tcgtttacgt gtacccgcaa aatagcgtac tccgttaatc gtaccgctga ttccagcacc
     2941 tgggtgggct gtaaaatcaa tcatatcgta tgttgctgct tcttgcagtt ttccgtattc
     3001 cacgattgct ttacctagtg gatgttcaga agcttgttct aaagtataaa aaatagatag
     3061 gacttcttta gaaccgacta gatctgtaac tttgggcttt ccttctgtaa tcgttccagt
     3121 tttatccaaa acaatgctgt ctaagtgagc agctgcttct aatgcttctc cgcctttaat
     3181 taaaattcca tttcgtgcac caacacccgt tcctaccata atagctgttg gagtcgctaa
     3241 acctaatgca catggacacg caatgactaa tacagagaca ctatggatca atgcgagttg
     3301 ccaatcacca gtgatcaaac ctgtagctat gagcgtgata aacgcgatga ctaatacgat
     3361 cggaacaaaa atccctgaga tccgatctgc gattttttgg attggggctt tgctcccttg
     3421 agcatcttcc accatttgaa tgatttgcgc taatactgtc tgcttgccga cttgtgtaac
     3481 ttccatgtga agtagaccat tcgtattgat tgtcccgcca aagagcgtgt catccggatt
     3541 cttttctaca ggaagatttt caccagtaag catactttca tctatcgcac tgcttcctga
     3601 aataattcga ccatctgctg gaatctgctc accaggacgg atgactagtt gatcaccaac
     3661 gactacttct tcaatcgcta acgtttcttc tttcccgttt cttatcactt gggcggtttt
     3721 tgtttgaaga gacatcaatt gtttgattgc attacctgtt ttcgtttttg ctgtatgttc
     3781 caaatatttg ccgagcaaaa tcagcgtgat gatcatactg ctgctttcaa aataaagatc
     3841 ttgggggtgt ccacggaaaa atccgttgta aatgctcaat gcaaaggcag ccgatgttcc
     3901 aattgctacc aatacatcca tatttggtgc tttcgtcttg attgcatggt acgcaccttt
     3961 ataaaaacga gcaccgacat aaaattggac aggagcagac agcactaact ggacgattgg
     4021 aaaatgaaaa aagtgaacaa tcgccgcatg gcttcctaac atcatagcaa tcattgacaa
     4081 catcaacggc aaagtcaaaa ccgtactcaa tataagatcg aatagcattt ttttcaaata
     4141 cgcttgcttt tcttctgcaa ttttttgttt gtgggcttca tcgtacaaaa ttgcaccata
     4201 gccgatgttt tctacgcttt ggattaattt ttcagtggtc gtcccttcat atttgacact
     4261 tgctttttca gtggccaagt tgactgtggc attgaccaca ccaggttgtc cgttcagctc
     4321 tttttcgatc ctagcagaac aatttgcaca agtcatacct gtaatgacga acgtatccat
     4381 tttggtttct gctgttgcca tcaaatcacc tctgctttat aacccaatcc attgattgct
     4441 tgacaaatct tttcggattg aacttttgtt tcatcaaact tgaccgtacc gtttgctttt
     4501 ttcaaattta ctttcacttt ttgcacgccg tcaagtgcgc ttactgtttc ttctactctt
     4561 gctgcacaat gattgcagct cataccttcg attgaaaatt gttgtttcat tttttcttcc
     4621 tcctattgat gttgtttaca ttcacattga cctgggatac agttacattc gatcgtatct
     4681 actggttgct tttgttccag ttgtttcaaa atctcattga tatcttcttg agtcaaagtg
     4741 gcttcttcta ctagatccgc aatcgtctct ccaatccttt ttgcacagat atgggaaaaa
     4801 agattttccg tcgcactgcg aactgtttcc atctccgaga cagctggata ataaatgaat
     4861 ttttttcctt cttgttctgt ccagatcact tcttttttta ccaaacgacc cagcaaagtt
     4921 ttgatcgttg ctggtttcca atccatcgaa tcagctaaga tctgcgtgat ttcttgggct
     4981 gtcgtctgtc caagtgtcca aataacacgc atgatctccc actctgaatc actgatcttt
     5041 actggcgttt tgactttttc catttatttc acacctttcg tttacaaatg taatcattat
     5101 cttatgaata cagattacac ttgtaaacaa aaactgtcaa ttatttttgc tctctatttt
     5161 caatttagtt aaaaattctt gtttctctcg tcttgtttta aggatgatca gtttatttct
     5221 tgcattagct acttggatca gctgctgtat tttcggttca ttgttttctt caaaatgcca
     5281 gataaaagat aaaaattcta agtattccct gtttagtttc tccgaaaatg attcaggcat
     5341 atctggccga gattttttgt ttatctttct ttttagagaa cgccaaacta ctcggtagat
     5401 ggcgagataa cgcggcactt taagccaaat gatcttatct gcttctttaa tcctttcctc
     5461 taatgtagaa gtataatttc cttcaacgat ccatgacgga ttagatacca taaacaattg
     5521 ctgttcttgc aaaaaccact tttttgcctg aatactgtag tctgttgtat gccataaaag
     5581 gtctagcggc agtagcggaa agtcggtgat ttctttgatc ttccttgcga gggtcgattt
     5641 tcccgaccca ctttgtccaa tcacgatgat cttcattctt ctccctcact tctctttgga
     5701 atttggttcg atcataccac ttataacgcc gcaaaaaaac aagggactgt gacaaaagtc
     5761 gtgtcaaagt cccttgtttc gaata
//
LOCUS       DACEEZ010000130         5694 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20313_2017
            SAMN12639966-rid9879223.denovo.133, whole genome shotgun sequence.
ACCESSION   DACEEZ010000130 DACEEZ010000000
VERSION     DACEEZ010000130.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639966
            Sequence Read Archive: SRR10023158
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5694)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5694)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741683.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741683.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:44:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,016
            CDSs (total)                      :: 2,962
            Genes (coding)                    :: 2,889
            CDSs (with protein)               :: 2,889
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 36 of 73
            Pseudo Genes (incomplete)         :: 29 of 73
            Pseudo Genes (internal stop)      :: 18 of 73
            Pseudo Genes (multiple problems)  :: 8 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5694
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639966-rid9879223.denovo.133"
                     /isolate="E20313_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(72..542)
                     /locus_tag="IWO89_002689"
     CDS             complement(72..542)
                     /locus_tag="IWO89_002689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4809 family protein"
                     /protein_id="HAQ0457383.1"
                     /translation="MKEVQLIRKSELSEGGCNACGVVEATSYTLKLDSNQAIISELTV
                     GGLVDSLALAEGFTGEDIYEMFSEVRQLKKGEKCIEVHHESPNVRFKRGDNEMIFKNH
                     VSDHTELYEIVNQILSGIFELGPYEFKEENGNPKLNEEWQETIETQRNNPHLFQ"
     gene            872..2512
                     /locus_tag="IWO89_002690"
     CDS             872..2512
                     /locus_tag="IWO89_002690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent malic enzyme"
                     /protein_id="HAQ0457384.1"
                     /translation="MLTGLNLLNNPFLNKGTAFTKEERAKYGITGMLPSTVQTLEQQS
                     VQAYGQYLSKQTDLEKRIFLMNLFNTNRTLFYKLMGEHLVEFMPVVYDPVVADSIEQY
                     NEIFLKPQDAAFLSIDEPENIKASLKNAADGRDIRLIVVTDAEGILGMGDWGVNGVDI
                     AIGKLMVYTAAAGINPAQVLPVSIDAGTNNEELLNNPLYLGNRHARVEGETYYEFIDQ
                     FVQSATELFPELLLHWEDFGRGNAAAILEKYEDQITTFNDDIQGTGIVVLAGVLGGLN
                     ISGEQLKDQIILTFGAGTAGVGIANILLDEMIRQGVPEEEARQHFYQVDKQGLLFEDT
                     EGLTPGQIPFARKRSDFTNADELTNLEAVVKAIHPTIMIGTSTQPGAFTEEIVKEMAA
                     HTPRPIIMPLSNPTKLAEAKAKDLIEWTDGKALVGTGIPAADVEYNGVTYQIGQANNA
                     LMYPGLGLGLIASTATRVNAEIISQASRALGGIVDVTKPGAAILPPVAKITEFSQTIA
                     ETVAKSVVAQNLNREEITDIKEAVESAKWVPEYKSLED"
     gene            2666..3988
                     /locus_tag="IWO89_002691"
     CDS             2666..3988
                     /locus_tag="IWO89_002691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxycarboxylate transporter family protein"
                     /protein_id="HAQ0457385.1"
                     /translation="MAQKTTTATAVEEKKKEGFWSTKITGVSLPLYLIMVLVLIIAMA
                     LGKLPTNMIGPIAVLVIFGNLFHFIGTKIPIVKSYLGGGSVFAIFASAAIATFGILPE
                     YVVKSTENFVSNMGFWDFYIAALITGSILGMNRNLLMKASVRFIPVALISMVVSFFAV
                     GLVGMLIGNGFANSVLYISLPAMAGGVGAGAVPLSTIYANVLGTDASSIISRLIPATA
                     MTNVLAIIGAALVARAGQSMPKFNGNGKLMEKGDLGDGKPRTVKPSIPQLGVGLMVSL
                     TFFILGQIGNYFVPKVHAYAFMIIIVVICKIANLLPEYYEDAAIMFNNLIVKNLTAAV
                     LAGIGIALLNLNVLASALTWQFVVLCLTSVIAISVVSGFVGRLFGLYPIESTITAGLC
                     NNSMGGTGNVAVLSAANRMELIAFAQMGNRLGGAIVLIISGFLMQLLS"
     gene            complement(4056..4490)
                     /locus_tag="IWO89_002692"
     CDS             complement(4056..4490)
                     /locus_tag="IWO89_002692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAQ0457386.1"
                     /translation="MLREMRLEDAEALREINADQLGYDIPLTVTVRQMKKLLKEPEKN
                     FFLVYEDPTSKKAAGYVQAESYESIFSDPMFNIMALAVSKYAEKRGIGKALMTGVEEE
                     AKKRGITTIRLNSAEYRVEAHQFYEHIGYHSDKMQKRFLKIL"
     gene            complement(4555..5392)
                     /locus_tag="IWO89_002693"
                     /pseudo
     CDS             complement(4555..5392)
                     /locus_tag="IWO89_002693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325086.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
BASE COUNT         1706 a         1137 c         1043 g         1808 t
ORIGIN      
        1 gcaggttttt cacgacctct gattcacggt gtttcttttt catcaacatt tttacgaaat
       61 actgtggaag actattgaaa taagtgtgga ttgtttcttt gtgtttcaat cgtttcttgc
      121 cattcttcat ttaattttgg attaccgttt tcttccttga actcataagg tcctaattca
      181 aaaatcccag acagtatttg attcacgatt tcatatagtt cagtgtgatc agacacatga
      241 ttcttgaaga tcatctcatt atcaccacgt ttgaacctca cattcgggct ctcatgatga
      301 acctctatgc atttttctcc tttttttaac tgacggactt ctgaaaacat ctcataaata
      361 tcttctccag taaaaccttc agcaagcgcc agactgtcca caagaccgcc tactgtcagt
      421 tcagagatta ttgcttgatt ggaatctaat tttaatgtat agcttgtcgc ttctactacg
      481 ccacatgcat tgcaaccgcc ttctgatagt tcgctttttc taatcaattg aacttctttc
      541 attttctctt cctcctctta attttctact tatgtatttt tatcacctga cataggtgac
      601 tcctatcaac gctatcatgt gcttttttcc tcagaaatcc tatttatcca ttccatctat
      661 cccttattct tctacttttc tttctctagc atataagaga tacgcatagt attcaagcaa
      721 ggattgtcga tataaaaaga acaaatactg ctattttgat ttcttttggc tatattacat
      781 ataaataaaa tttatcatac ctgctctttt tgactatatc atcatctgac ttctatactt
      841 cgtttgtatt aaaacttagg aggaatcatt catgcttaca gggttaaact tattaaataa
      901 tccttttcta aataaaggaa cagcttttac aaaagaagaa cgggcaaaat atggaatcac
      961 tggtatgctt ccaagtactg tccaaacact ggaacaacaa tctgttcaag cttatggtca
     1021 atacttaagc aaacaaacag atttggaaaa acgtatcttc ttaatgaatc tattcaatac
     1081 gaatcggaca ttgttttata aattgatggg cgaacattta gttgaattca tgcctgtcgt
     1141 ttatgatcct gtcgttgcgg attcaatcga acaatacaac gaaattttct tgaaaccgca
     1201 agatgctgca tttctttcaa tcgacgaacc agaaaacatc aaagcaagtt tgaagaacgc
     1261 ggcagatgga cgagacattc gtttgattgt tgtgactgat gcagaaggaa tcttaggtat
     1321 gggcgactgg ggtgtgaacg gtgttgacat cgctatcggc aaattgatgg tctatactgc
     1381 tgctgctggt attaatccag cacaagtttt gcctgtatcg atcgacgctg gtactaataa
     1441 tgaagaatta ttgaataatc ccttatactt aggcaatcgt catgctcgtg tagaaggcga
     1501 aacttactat gaattcatcg atcaattcgt tcaatcagct accgaattat tcccagaatt
     1561 gttattgcat tgggaagatt ttggacgagg aaatgctgca gcgatcttag aaaaatacga
     1621 agatcaaatc actactttca acgacgatat ccaaggtaca ggaattgttg tcctagcagg
     1681 tgtattaggc ggattgaata tatccggtga acaattgaaa gatcaaataa ttttgacatt
     1741 tggtgctggt actgctggtg tgggtatcgc aaacatcctt ttagatgaga tgatccgtca
     1801 aggtgtacct gaagaagaag cacgccaaca tttctatcaa gtagataaac aaggtttatt
     1861 gtttgaagat acagaaggat tgactcctgg tcaaatccca ttcgctcgca aacgctcaga
     1921 tttcactaat gctgatgaat tgacaaactt agaagcagtt gtaaaagcga tccatccgac
     1981 aatcatgatt ggtacttcta cccaacctgg agcattcact gaagaaatcg tgaaagaaat
     2041 ggctgctcat acaccacgtc cgatcatcat gcctttgtct aatccaacaa aattggcaga
     2101 agcaaaagca aaagatttaa tagaatggac ggacggaaaa gcattagttg gaacaggtat
     2161 ccctgctgct gatgttgaat ataatggtgt aacttaccaa atcggtcaag caaataatgc
     2221 tttgatgtac ccaggtttag gacttggttt gattgcttca acagctacac gagtaaatgc
     2281 tgaaatcatt tctcaagcta gtcgagcatt aggcggaatc gttgatgtaa caaaaccagg
     2341 agcagctatc ttgcctccag tagcaaaaat cactgaattt tctcaaacga ttgctgaaac
     2401 tgtcgcaaaa tcagttgtcg cacaaaactt gaatcgtgaa gaaatcacgg atatcaaaga
     2461 agcagttgaa tctgctaaat gggttcctga atataaatct ttagaagatt aagatcgtta
     2521 tagtaaaaga acatacagaa actttgtatg acgtctaaaa agaaaaaagc gactgcggca
     2581 aaacagcggc cagccaactc ctgctctttt gtcgcttttc tttggctttt ttcatatgat
     2641 acagaataag aaaggatctg taaaaatggc acaaaaaaca acaactgcaa ctgcagttga
     2701 ggaaaagaaa aaagaaggtt tttggtccac aaaaatcacc ggcgtaagtt tgccacttta
     2761 tttgatcatg gtccttgtcc tgatcattgc aatggcttta gggaaattac cgacgaatat
     2821 gattggacca atcgcagttt tggttatttt cggtaattta tttcatttta tcgggacaaa
     2881 aatccctatc gtcaaaagtt atttgggtgg tggttccgtt tttgcgatct tcgcttctgc
     2941 tgcgattgct acatttggga tcttacctga atatgtagtg aaatccacag aaaactttgt
     3001 cagcaatatg ggcttttggg acttttatat tgctgccttg attactggta gcatccttgg
     3061 tatgaaccgt aatctattga tgaaagcttc tgttcgtttt attcccgttg cacttatttc
     3121 aatggtcgtc tccttttttg ccgtaggact cgtgggcatg cttatcggaa atggttttgc
     3181 aaattccgtt ttatacatct cattacctgc gatggctggt ggtgttggag ccggagccgt
     3241 tcctttatca acgatttatg caaatgtttt aggtacggat gcttcttcta tcatttcccg
     3301 cctgattcct gcgacagcaa tgacaaatgt cttagctatc atcggtgcag cattagttgc
     3361 tcgtgctgga caatcaatgc caaaattcaa cggcaatggt aaattaatgg aaaaaggcga
     3421 tcttggcgat ggaaaacctc gaactgtgaa accttccatt ccacagttag gtgttggttt
     3481 gatggtatca ttgactttct tcatcttagg acaaattggt aactattttg ttcctaaagt
     3541 ccatgcttac gcatttatga ttattatcgt tgtcatctgt aaaatcgcca acctattacc
     3601 agagtattat gaagatgcag ctatcatgtt caataacttg attgtcaaaa acttgacagc
     3661 agctgttctt gccggtatcg gtattgcgtt gctgaatctg aatgtattag cttctgcttt
     3721 gacatggcaa tttgttgtct tatgtctgac aagtgttatc gcgatttcag ttgtttcagg
     3781 atttgtcggt cgtttgtttg gtttatatcc aattgaatca actattactg ctggcttatg
     3841 caacaactcg atgggcggaa caggaaatgt agccgtctta tctgcagcta atcggatgga
     3901 acttattgct tttgctcaaa tgggcaatcg acttggtggt gccatcgttt tgatcatctc
     3961 tggtttcttg atgcaattac tttcttagtc aaaaaaacaa aaatacctga tcatcaagta
     4021 tcggcttggt gatcgggtat ttttttgata aagatttaca ggattttcaa aaatcttttt
     4081 tgcattttat cggaatgata accgatgtgt tcataaaatt ggtgtgcttc tactctgtat
     4141 tctgcagaat taagccttat tgtcgttatt cctcgttttt tcgcttcttc ttctacgcca
     4201 gtcatcaaag cctttccgat gcctcttttt tcagcatatt tagacacagc aagtgccata
     4261 atattgaaca ttggatcgct aaaaatcgat tcataggact ccgcttgtac atagccagct
     4321 gcttttttag aagtaggatc ttcataaacc aagaaaaaat ttttctctgg ctccttcagc
     4381 agtttcttca tttgtctcac cgtaacggtt aatggtatat cataccctaa ttgatccgca
     4441 ttgatttcac gtagtgcttc tgcatcttct aaacgcattt cccgtaacat ccaaatcacc
     4501 agccaatttc tgatttttta aaatgaattt ttttatcata gtatactctt tttttcattt
     4561 agaacaatat gtttcagtga ttttgacttg cgttagtcta gattctccta atgtttgtaa
     4621 gatgacaaag gcattgtaac aattgaccaa aagaaccacg aggacaatta cttgaaaaat
     4681 aaaggtcact ttttttggag acaaaaccct gctaaatctt gctccccaca aacctccaat
     4741 aacagcagcg gggataacat aaaacaacat agataaatca aagcgcataa agctacttgt
     4801 taaagcaatc gttaccaatt ttgccagttg agaaaagaaa atcgtagata gagaatatag
     4861 tgttgcttct tttattggca atgcaaacat cagcatcaac aaagaaacat tgactggtcc
     4921 accaccaata cctaaaaaac tagctaaaaa gcccagaacc ccccccacat ataaggtacc
     4981 aagtccatga cgtcaaatga aattttggct gatgatgctt ggtgtagaag aacgcaaaaa
     5041 ccaaggtcaa taccgtcaaa aaaatttgga tcagctgcac atgcttctcg ttttcaaata
     5101 actgtaaaaa aacttcgaat acaatgttgc cgactactcc tccaacaaca gctccactag
     5161 atacccaacc gacaatctgc caattcagtg atttcccgct tgatacttgt cgcacagtcg
     5221 aaacaatgga catcgtgaat accgcaactg ttgagtaaaa agaaatccct gcaacagaat
     5281 gggcgccgat aagatcaaga atcggtttga tcaacacgcc tccacccatc ccggaaatag
     5341 cgccaactgt gttcgccaga acgatcacca caaaataaat cagaccaatc atcttgtatt
     5401 tcctttctct tgtttatatc ttttagcgat tgttactctt ttcttttatt atacagtaaa
     5461 agaaacaaac gaggcttcga aagcttttct actttttaag gaaatttagt gctaaacgaa
     5521 aagaggttgt gaaaaagctg tcctgcatca agtaataaga acagccaaac aaaaatagct
     5581 cctcataatt ttcgtttggt tgaacttatt accgcagatg caagcttttg aacaccgttt
     5641 aagaaagagg tcgtgagcct gccgttcagc tccgagtcac aaggaacaat ttca
//
LOCUS       DACEEZ010000131         5458 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20313_2017
            SAMN12639966-rid9879223.denovo.134, whole genome shotgun sequence.
ACCESSION   DACEEZ010000131 DACEEZ010000000
VERSION     DACEEZ010000131.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639966
            Sequence Read Archive: SRR10023158
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5458)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5458)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741683.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741683.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:44:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,016
            CDSs (total)                      :: 2,962
            Genes (coding)                    :: 2,889
            CDSs (with protein)               :: 2,889
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 36 of 73
            Pseudo Genes (incomplete)         :: 29 of 73
            Pseudo Genes (internal stop)      :: 18 of 73
            Pseudo Genes (multiple problems)  :: 8 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5458
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639966-rid9879223.denovo.134"
                     /isolate="E20313_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(60..2009)
                     /locus_tag="IWO89_002694"
     CDS             complement(60..2009)
                     /locus_tag="IWO89_002694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit"
                     /protein_id="HAQ0457387.1"
                     /translation="MENSTEFFKNIQTEFNHALTPMKPPKHKLVGRDDELHELSLILN
                     RPETPQAILLGEAGTGKTALASEWAERQRQDGKKVQVYKLSIGQLRVGGDDVLQTRLE
                     TLIPRLKEYERILKAEDKDNEFVLFIDEFHKVVSSFGSGNKIGGDSLKEPLANESLQM
                     ITATTRKEYENYIKRDDALGRRLQPLELNELKSDVVVDILRQWLNTSSVKDDPQASFR
                     LSQRIPDELLQEIVQANKLYREDLSEPAKSIAVMEDLIAKDRVDGNAYNRESLLQIFG
                     KYNIQLDSDFDMEKLQEELKSIKGQPLAVYTITMLFKRMKLGFDRFKGRKQPKATALF
                     TGSTGVGKTAMAKAVARGLYGDENKVLLVNMPDYKTKDDAMRFRRLIGTHVRHDPSAI
                     IVCDEYEKAGVETLDAFLSILDEGRISFTERGWDDREVHDTVSLSNTILFATTNAGAE
                     MFDSLNRYSSLSRVTNKENSNEYSTELKREYRKVASGIKQALIGEGLKPEVINRFGRI
                     VPFWALSEQTLLEIAYNEMLKSAELVRKHLNIDLRLPPRKERVYGQNFYADDLSMHLV
                     FDMASQEDSKSGGARNILHLVESEFEEAITEAIITYPNAKIFDVKLSEAGAFEGKGDS
                     NAEIIAKPSVAESKENKIVFSIGGA"
     gene            complement(2051..3109)
                     /locus_tag="IWO89_002695"
     CDS             complement(2051..3109)
                     /locus_tag="IWO89_002695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FliS"
                     /protein_id="HAQ0457388.1"
                     /translation="MEKNVNSTSTKDQYSIIGGLLGDKDKRTRHGLRIMLATFLGVLF
                     LLTGLGSYIYQLGNYNYYLVNNTTPIGSSLEFARSGAEITISDVWTDQNRDVTVVKLG
                     YSQNARKLLSTQGKNYNLYMATKEKDRPKKLEMSYGMLSTDGDAFLFLKGKLEERAYQ
                     VFIANQVSLISGDDTADDDTKVNVNEADSVTKALSQYSMNDVNNEGVISFGKKAKPVN
                     TPDNINFRINPYSDSTNVYQGSFLTKDGDIDYSKVVAVTSKNNVVNKLEDQLNASNTN
                     IKKIEATKAEYLERLQQNPDDKDSQESIRQLDSRMETEIKAINDTQKLIDQYKNADFN
                     KDSFGDMKTTFKYLTLTD"
     gene            complement(3228..3683)
                     /locus_tag="IWO89_002696"
     CDS             complement(3228..3683)
                     /locus_tag="IWO89_002696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0457389.1"
                     /translation="MNAINWHEFVNYMESLLDDLKSFSIADAVKHGLPVSTTMKLRGL
                     KIPDEIIKLRTKTIIETAKVVDNMNHELKGKKTTYEKDFERFLKSVIGKYLGKKSFSE
                     LRDEEIVPTPMVSKIRKNKAEGNYYLGTIAKIIDYLENKEQKKGTKKGR"
     gene            complement(4267..5070)
                     /locus_tag="IWO89_002697"
     CDS             complement(4267..5070)
                     /locus_tag="IWO89_002697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HAQ0457390.1"
                     /translation="MSQEVTEKIIDSDVDLVSEQTNAIVRYNNGLNTTALRGFKPVEM
                     DVFWAILSKMKRQGTAEVTFDFNLFRYLVKYDRREKDSFYSVLKALSDKLGTLTYRFE
                     DSDVYEQLWLFQKFRIDKQKETVTIKANEEFEFILNSIGKNFTRFELDNFTKLRSSYT
                     KELYRHLMQYRNKEWRNGYWVVKVEDFRRALSIPSSYRMTNIDKRILEQAKEEFLAPD
                     ENGIQVFETFDYEKIYAKKGRRVDRLRFTFSEPESNLPTISMHNWLEED"
BASE COUNT         1591 a         1087 c          757 g         2023 t
ORIGIN      
        1 tcttacgacc catcgtttta ttctaaggtt ttgtagtaaa tatttcttga atttcatagc
       61 tacgcccctc caatcgaaaa tacaatcttg ttttctttac tttcagcaac ggaaggcttt
      121 gcaataattt cagcattaga atcacctttt ccttcaaacg ctccggcttc actcaactta
      181 acatcgaata tttttgcgtt tggataagta attattgctt cagtaatcgc ttcttcaaac
      241 tcagattcaa ctaaatgcaa gatattcctt gcaccccctg atttagaatc ctcttgtgaa
      301 gccatgtcaa aaactaagtg catggagaga tcgtctgcat aaaaattttg accatatact
      361 ctttctttcc taggtggaag acgtaaatct atatttaaat gttttcgtac taattcagca
      421 ctcttcaaca tttcattata agcaatttcc agaagagttt gttcggataa agcccaaaat
      481 ggaactattc gtccaaatcg gttaataact tcaggcttta gaccttcacc aatcaaggct
      541 tgtttgatcc ctgatgcgac ttttcggtat tctcttttta gttcagtgct gtattcatta
      601 gaattttcct tgtttgtaac acgagatagt gagctatatc ggtttaacga atcgaacatc
      661 tccgcacctg catttgttgt cgcaaataaa attgtatttg acaatgaaac ggtgtcgtga
      721 acttctcgat catcccatcc tctttcagta aaacttatcc taccttcatc taaaattgat
      781 aaaaaggcat cgagtgtttc tacaccagct ttctcatatt catcacacac aataatcgca
      841 gaaggatcat gccttacatg agtacctata agtcgtctaa aacgcattgc atcatctttg
      901 gttttgtagt ccggcatatt aactaagaga actttatttt catcgccata aagtcctctt
      961 gccacggctt tagccattgc agttttaccg acccctgttg atcctgtaaa cagagcagtt
     1021 gcttttggtt gctttcgacc tttaaaacga tcaaatccaa gcttcatacg tttaaacaac
     1081 attgttatag tataaactgc taacggttgt ccttttatgg actttaattc ctcttgaagt
     1141 ttttccatat caaaatcaga atctagctga atattatact taccaaatat ttgcaataga
     1201 ctttctcgat tataagcatt tccgtctact cgatcttttg caatcaaatc ctccattaca
     1261 gcaattgatt tagccggttc ggacaaatcc tcacgataaa gtttgtttgc ttgtacgatt
     1321 tcttgaagta attcatcagg aatcctttgc gataatctaa aagaagcctg aggatcatct
     1381 ttaacagagg atgtatttag ccattgtctc aaaatatcga cgacaacatc gctttttagt
     1441 tcattcaatt ctaacggttg tagccgtctc cctaaggcat catctctttt gatatagttt
     1501 tcatattctt ttcttgtagt agcggtaatc atttgaagtg attcatttgc taacggttct
     1561 tttagcgaat cacccccaat tttattgcca ctcccaaaag agctaacaac tttatgaaac
     1621 tcgtcaatga agaggacaaa ttcattatct ttgtcctcag cttttaaaat tcgctcatat
     1681 tctttcaatc ttggaattaa tgtttctaaa cgtgtttgga ggacatcatc tcccccaaca
     1741 cgcaattgtc cgattgataa tttatatact tgtactttct ttccatcttg tcgctgtctt
     1801 tcagcccatt cacttgcaag tgctgtttta cctgttccgg cttcacctaa caagatagct
     1861 tgcggtgttt ccggtctatt caagatcagt gacaactcat ggagttcgtc atcacgtccc
     1921 actaacttat gttttggtgg tttcattggt gtgagagcat gattaaattc ggtttgaata
     1981 ttcttaaaaa actcggtact attttccatc tgtttgtgtt cacttcctct aaaataagaa
     2041 taatttactt ctagtcagtc aaagttaggt acttaaatgt cgtcttcatg tctccaaaag
     2101 aatctttgtt aaagtcagca tttttgtatt gatcaatcaa tttttgtgta tcattgattg
     2161 ctttaatttc ggtctccatt cgactgtcta gttgacgaat actttcttgc gaatctttat
     2221 cgtcaggatt ttgttgtaaa cgttccagat actcagcttt tgtggcttca attttcttga
     2281 tgttagtatt tgaagcattt aactgatctt ctagcttatt tacaacatta ttcttacttg
     2341 taactgctac aacttttgaa taatcaatat cgccatcttt ggtaagaaag cttccttgat
     2401 agacattagt tgaatctgaa tatggattta ttctgaaatt gatattatca ggagtattaa
     2461 caggttttgc ttttttccca aaagaaatta ctccttcatt attaacatca ttcatagaat
     2521 attgagaaag tgcttttgta actgaatctg cttcattcac attgactttg gtatcatcgt
     2581 cagctgtatc atcaccagaa attagtgata cttggtttgc aataaaaact tgataagctc
     2641 tttcctctaa tttacctttt aggaataaga aagcatcccc atcagtactt aacatcccat
     2701 agctcatttc aagcttctta ggacgatctt tttcttttgt tgccatatat aagttatagt
     2761 tttttccttg cgtagataac aactttctag cgttttggga gtaaccaagt tttactactg
     2821 ttacatctct attttgatct gtccacacat ctgaaatagt gatttctgct cctgatcgag
     2881 caaactccaa tgaagaccct ataggtgttg tattgttcac gagataataa ttatagtttc
     2941 ctaattgata aatgtagcta cccaagcctg ttaataaaaa aagcactcct agaaaagttg
     3001 caagcataat gcgtaatccg tgacgtgttc tcttatcctt atcaccaagc aaccctccaa
     3061 taatactgta ttgatctttg gtagatgttg aattgacgtt tttttccatc agtttgtctg
     3121 ccccctttca taaaagagca aacaatcaga cgttttttcg gtaattctag gttcgggatt
     3181 ttgaatcatt tcggtatatt cttccccatt aatatttaac acaaaaatta tcttcctttc
     3241 ttcgttcctt tcttttgctc tttattttcc aaataatcaa tgatttttgc tattgtacca
     3301 agatagtaat tcccctcagc tttgtttttt cggattttgc taaccattgg agtaggtaca
     3361 atctcttcat ctctcaattc actgaaagat tttttaccta gatattttcc aataacagat
     3421 tttaaaaaac gttcaaaatc tttctcgtaa gttgttttct tgccttttag ttcatggttc
     3481 atattgtcaa cgacttttgc ggtttcaata attgttttag ttctcagctt aataatttca
     3541 tctggaattt tcaatcctcg taatttcatc gttgtactaa ctggtaaccc atgctttact
     3601 gcatcagcaa tactaaaact ttttagatca tctagtaagc tctccatata gttaacaaat
     3661 tcatgccaat taatagcatt cattgtctca ccccttcatt tttttaaaat gaggttcggg
     3721 cgtaacacat ttatgttatt ataatatcat aataacataa ataaatcaat cagcaaattt
     3781 agatatccct taaagtatca gcaattattc aacaagatat ttattttagc tatttcagtc
     3841 atcataaaag actaattagc aataaaataa ctaatattat ccatttatca gacgaaaaga
     3901 aaaaacttat ctaattactc tatattaact tttaacaaat agttgacttg tccaaaaatg
     3961 ttaccttcaa tctcacaaag atattaagat gtttcctacc aaaactgata tcaccggact
     4021 ttttattctg tgttgtccaa aagtgttacc tttagtcgat caacaaatta aaaaaaattg
     4081 tttttttctt gacttgtcca aaagtgttac ccatagttgt taaaatttgt atagattagc
     4141 cattctttcg aatattattt agactgaaac ttgcttacaa aaaaacttct tgcatttatt
     4201 gtccaaaagt gttacccata cttttttaaa gaagcaggtt gtattaaaat tacaacctgc
     4261 ttcttctcaa tcttcttcta accaattatg cattgaaatg gttggcaagt ttgattctgg
     4321 ctcactaaaa gtgaatctga gccgatcaac tcttcttcct ttctttgcat agattttttc
     4381 ataatcaaaa gtttcaaata cctgtattcc gttttcatca ggagctaaaa attcttcttt
     4441 cgcctgctct agtattcttt tgtcaatatt tgtcattcta taacttgaag gtatacttaa
     4501 tgctcttcta aaatcttcaa ctttgactac ccaataacca tttcgccact ctttatttct
     4561 gtattgcatg agatgacgat acaattcttt tgtgtagcta cttcttagtt ttgtaaaatt
     4621 atcaagttca aaacgagtaa agtttttgcc aattgagttt aagataaatt caaattcctc
     4681 atttgcctta attgtaactg tttccttctg tttatctatc cggaattttt gaaaaagcca
     4741 cagctgttca tagacgtctg aatcttcaaa acggtaggtt aaagtaccta gtttgtcact
     4801 caaagctttt aacacagaat aaaaactgtc cttttctctt ctgtcgtatt tgaccaaata
     4861 acgaaagagg ttgaaatcaa aagttacttc tgctgttcct tgacgtttca tttttgataa
     4921 aatagcccaa aacacatcca tttcaacagg cttaaatcct cgcaaagctg ttgtattcag
     4981 accattgtta taacgcacaa ttgcatttgt ttgttctgat actaaatcta catcactatc
     5041 aataatcttt tcggtaactt cttgagacat tgattactct ccaatcttct ttttgcgctc
     5101 ctttaatcat gttaatctgg tactaattaa aggaaacaat tttttctgct tgttacctaa
     5161 attaaatcac aactaaaggt aacagtcaac aaagatttgt tactaatcta attttctatt
     5221 ttttcgcctt ccaaatcaag aaaaccgaag gtaacgtctt atcctagtgt gatacctcaa
     5281 atgtccactt ttgttacctt tgctgtccaa aagtgttacc cttactgtcc acttttgtta
     5341 cctttactgt ccaaaagtgt tacctttata cccctttaat acttactctc ccaagagatt
     5401 aagcctcccc taacagatat attaacagat atttaacaga tatattaaca gatattta
//
LOCUS       DACEEZ010000132         5341 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20313_2017
            SAMN12639966-rid9879223.denovo.136, whole genome shotgun sequence.
ACCESSION   DACEEZ010000132 DACEEZ010000000
VERSION     DACEEZ010000132.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639966
            Sequence Read Archive: SRR10023158
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5341)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5341)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741683.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741683.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:44:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,016
            CDSs (total)                      :: 2,962
            Genes (coding)                    :: 2,889
            CDSs (with protein)               :: 2,889
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 36 of 73
            Pseudo Genes (incomplete)         :: 29 of 73
            Pseudo Genes (internal stop)      :: 18 of 73
            Pseudo Genes (multiple problems)  :: 8 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5341
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639966-rid9879223.denovo.136"
                     /isolate="E20313_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(62..280)
                     /locus_tag="IWO89_002698"
                     /pseudo
     CDS             complement(62..280)
                     /locus_tag="IWO89_002698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391850.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     gene            complement(546..740)
                     /locus_tag="IWO89_002699"
     CDS             complement(546..740)
                     /locus_tag="IWO89_002699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0457391.1"
                     /translation="MMGFFLRFYIIELTGRLTVFFGNFAIKPFAEVTAHYICKWLDCK
                     NKIKKVFHYISSSPCQIRPG"
     gene            2092..3132
                     /locus_tag="IWO89_002700"
     CDS             2092..3132
                     /locus_tag="IWO89_002700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein RepA"
                     /protein_id="HAQ0457392.1"
                     /translation="MTDFKFFKADRVYNELFYQFPKVFIVSDEYKKMKDSTKIAYMLL
                     KARLEIAISKRQIDEEGNVYFTYTTNELCRVLNCQKQKAIAIKKELESFGLLLQKQMG
                     FNKQLGKNNPNRLYLAELKVSENDIYLLEKFDRENRENVDKSEGMKIIPTLDEKSDAE
                     SLGAQEGMKIIPCQNVDKSEGMKIIPELNNNILDTNRHNIDTEKDRLQDQLLLDNFET
                     IMTNDSIATFVPERVLNLIKTFSSSYSEAQKTVQTIHNAKKKAEIESGISIVFEELDS
                     YYVNAEQELYTTLLKAYQKLKTEKVENIQNLIFVYVKNWFIEKPIAAKVSSEKRLNYE
                     SSPSTITKDWLE"
     gene            3956..4288
                     /locus_tag="IWO89_002701"
     CDS             3956..4288
                     /locus_tag="IWO89_002701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraE"
                     /protein_id="HAQ0457393.1"
                     /translation="MSYTGILSLKDICHYGKRCTATEKITKKLSTGQSKTVVQCKKYI
                     IQKDKVSEEMIYYIGKQKQIILKDPIPLKELYPTIKHVYDQNGVLIGRRKNGVLRCTA
                     KGMGRLIG"
     gene            4907..5248
                     /locus_tag="IWO89_002702"
     CDS             4907..5248
                     /locus_tag="IWO89_002702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5406 domain-containing protein"
                     /protein_id="HAQ0457394.1"
                     /translation="MKNYDPNIRLGTHTIKVSFQRWDYKGFVTFRRGGNCKGLDVLAL
                     DEDDLYDQTLTDNPIGFGLLPEDDEGNEWFKMTLMNDNGDELSVEDTWSYLSDYIVSF
                     EIIEFVADKEE"
BASE COUNT         1943 a          728 c          900 g         1770 t
ORIGIN      
        1 taggtatttt tattatatat agatttaaca tatgtcggtc ggttgattga tatagctaaa
       61 gttataactc aaccgaagcc gattcatcta cacgaataaa gttgcgagtg ttctcggcta
      121 attcataaaa attgttatgt gttccatata gcaatactgg aataacttta ttttcttgaa
      181 tctcataagc aactctatac acggtgaccg catacttaaa tccattagac caattacctg
      241 ataaacctcc agttttttgc tcttacttaa atggatcaat ggcaatttca tcacaaacct
      301 atagaggatg agtgacttct gcctaattac gttaaaaaat attgaaagta actctatgta
      361 gcaaaaaata tctagaattt catttcctca aaactgatcg atattgtatc taaaaatata
      421 tactacctaa aataggtagt atatattaaa atatgtgctt ttgttagaaa aatcaataca
      481 taaattatca tatctgtata tgtcatttag gtatttcagt tatgttataa tagttttgct
      541 gtctgctacc ccggcctaat ctggcaaggg gaggaggaga tataatgaaa tacctttttg
      601 attttgtttt tgcaatcaag ccacttgcag atgtagtgtg cagttacctc tgcaaatggc
      661 ttgattgcaa aattgccaaa aaagacagta agcctacccg ttaattctat aatatagaaa
      721 cgtaaaaaaa accccatcag cctgcacgct gatggggttt ggtgatataa tgaaatacct
      781 ctttgttact cataattgta ccataattag tcacgaatgc aaccaataga aaaaactact
      841 tagaacggta gatcatcgct aataacaatg cattgtccac caaatggatt ttgtgaagca
      901 ttaaatacat tgttattaat tgtcgctgct tttaactagg cctctcccct tctattataa
      961 aagaagatat atatcatttc ttaattacct actaccttaa accaaaattt aagtactacc
     1021 taatttaagt attacttaaa caatctaaaa aatagttatc ccaattgtta tgcttttata
     1081 atacgattat ttgtaacaaa atgaatatta tataattaaa cactaccata tttaggtagt
     1141 gtttaattat tagcaaaaca aaaaatattt attctattct cttaacttca actaagttat
     1201 gctgcacacc tttatataaa tcatatgcaa ctcattagct agacaaacta caagcatgta
     1261 gttttatctg ttaattatgt actacctaaa taaagtagtt tataattagc aagtatatat
     1321 atttaaaaat ttaagattaa tttttttatg acttgtacaa atttatctga tattctctta
     1381 ttaagaacta cctaatttgg gtagttctta ttttttacat tttaagtagt atttaattgc
     1441 aatttggtag tattttttta ttatcttatt cttagaacgg tagtacttaa tataaatata
     1501 ggtagtatct aatttaagta ttaatggtag tatctaaatt aacaggtagt atgtaactac
     1561 tttaaagtaa tttttttcag attaacacag tagtatctta ttaaataagt taattttaat
     1621 ggtagtacat aattattata tatatctgac attggtagta cttattgtat tttttagtag
     1681 tatataatct attgttttct agcctaaagc cttgtcttat aagaataata taatatatac
     1741 tactgaaata aggtagtata tattactagt aatggtagta tgttttatat gcataaggta
     1801 gttcttaatt tattttttgt tttgacactg gtagtactta attatcttta attttaaaac
     1861 taaattttat tcatacgttt ttctggtagt atttaattat aaataatatt tatttaaagt
     1921 agttcttaat gactttaaag gtagtacata attcatttta tttctgattt taggtagtat
     1981 ttaattaaaa aacagtttta atgcgtttgc attttttatt tatctttgct attttcgaaa
     2041 agagtaggta attttacata aataaaacaa cgtgaaagta gaggatctac aatgactgat
     2101 tttaaatttt ttaaggcgga tagagtttac aacgaattat tttatcaatt tccaaaagtc
     2161 tttattgttt ctgacgaata caaaaaaatg aaagattcaa ctaagattgc ctatatgctt
     2221 ttaaaagcaa gattagagat cgcaatcagc aaacggcaaa tcgatgaaga aggtaatgtt
     2281 tattttactt atacgacaaa tgaactatgt agagtattaa actgccaaaa acaaaaagcg
     2341 atagcaatca aaaaagagtt ggaatccttt ggtttattat tacaaaagca gatgggattt
     2401 aacaaacagt tagggaaaaa taatcctaat agactatatc tagcagaatt aaaagtctca
     2461 gaaaatgata tctacttact cgaaaaattt gatagagaga atagggaaaa cgttgataaa
     2521 tcagagggta tgaaaatcat acccaccctc gacgaaaaat cagacgctga atcccttggg
     2581 gctcaagagg gtatgaaaat cataccgtgc caaaacgttg ataaatcaga gggtatgaaa
     2641 atcataccag aacttaataa taatatatta gacactaata gacacaatat agacactgaa
     2701 aaagaccgcc tacaagatca attgttgtta gacaattttg agacaattat gacaaacgac
     2761 agcattgcta cgtttgtccc tgaacgagta ttaaatttga taaaaacatt ttcttcaagt
     2821 tacagtgaag ctcaaaaaac cgtccagact attcataatg caaagaaaaa agctgaaata
     2881 gaaagtggta tttcgatagt ttttgaagaa ctcgatagtt attatgtcaa tgcagaacaa
     2941 gaattataca cgacactgtt aaaagcctat caaaaattaa aaaccgaaaa agtcgaaaat
     3001 atccagaacc tgatttttgt ctatgtaaaa aattggttta tcgaaaaacc aatagctgct
     3061 aaagtatcaa gtgaaaaacg tttgaattat gaaagctccc caagcactat tacgaaagac
     3121 tggttagagt gaaactttac tgacaaagtg attgttgctt catggagtaa cattaaaatt
     3181 aaaaattttt tattattcta attatattac gaatattaca aaataatagc tattttaata
     3241 gaatttataa gttaaaatat tatgataaaa atcacaaacg tagcctttta cgtcataagt
     3301 gttaaaagaa ataattaatt tcaaatatag aaaagggaac gtatgtttgc atactccttt
     3361 tttttgagtt ataataatac gcgaagagag ataatttcat tatttaaaca agctaaaaat
     3421 cttgatatta cttcttttca ggcaatacga atattattcg cttacatttt ttgtataaat
     3481 ttaattgtct gctatgcttg tgatgaattg attttcgttc tgcacttttg gatgataaat
     3541 ttggacaatt gaaaatacaa aaaaaagaaa ggcggtatga actcggaagg ctggtaacct
     3601 tcgtcagcgt tcatacacgg cattaagatt tggcccttaa tgcgttaacc tttcagtgtt
     3661 acggtattac ccgcaatgga tttagtataa cagatattca aaagtgatac cacttttttt
     3721 ggctgtgtta aggttaaaaa tacgtgatat tacgatgaat ctatgcctaa gcacaatgtt
     3781 ataaaaatgt ccgtgtggct ctttgccgta tgacgaacgc tgaaattaga gggaacgcat
     3841 gtttggatgc gttccctctt ttttgtttgt catgaacgaa aatcaattct ctatttttga
     3901 aaaataacaa gaaaaacttg ttaaaaatat ttaaagaaat tgggtggttt attttatgtc
     3961 atatacagga attttgagtc tcaaagatat ttgtcactac ggtaaacggt gtaccgctac
     4021 agagaaaatc acgaagaaac tatcaactgg ccaaagtaaa accgttgtgc agtgtaagaa
     4081 atatataatc cagaaagaca aagtctcaga ggaaatgatt tattacattg ggaaacaaaa
     4141 gcaaataatt ttaaaagatc ctatcccctt aaaagagcta tatccaacaa tcaaacatgt
     4201 ttatgaccaa aacggtgtac tgattggtcg aagaaaaaat ggagtcttgc gttgtaccgc
     4261 aaaagggatg gggcgtttga tcggctgaat tgatcattct cataacttgt attttatcgt
     4321 ttttcgcttg cttgaaaata ttgtcgagac ttctaaagga acttagagtc actagaaaaa
     4381 taaggagtag acttgatcta tcaacaattc ccttcgggga gtgacggggt aggttttaac
     4441 gattggtaac gtaaaaaatt ggtcaatcga gtaaatgcgc ttgtttggat agtggtgaaa
     4501 tgaatttgtg taaataataa gcgcagagta aacaagaggg aatttagaag ttagacaagt
     4561 tagaatagcg tctagattaa tcatgagcag ctactaaggg atctagactt tgacgcaaac
     4621 tcatggaata aggagtaggt cttctcgtaa tggggaggac gcacaagaaa ttgtgtcgtg
     4681 tgctgcagta catgtagata cttgcgagga cgttgattat tggatcaatc gagcatttac
     4741 cgctgtctac tagggtttct cgtttcggac gccataaacg agtcgaaatt ttttatgtat
     4801 ttttaagtaa acgaaaaacg ataaaatatg aacaagtcct gaataaagtg aaaaaattga
     4861 tataaaacat agagaaaatg aaatttacgt aatggaggaa tgacaaatga aaaattatga
     4921 tccgaatata cgattgggca cacatactat taaggtttct ttccaacgtt gggattacaa
     4981 aggatttgtg acgtttagaa gaggcggaaa ttgtaaagga ttagatgttt tagcattaga
     5041 cgaggatgat ttatacgatc aaacattgac tgataatcca attggattcg gcttgctacc
     5101 tgaagatgat gaaggtaatg aatggtttaa aatgacgctt atgaatgata atggagacga
     5161 attatctgtt gaagatactt ggtcatattt gagtgattat attgtcagct ttgaaatcat
     5221 tgaatttgta gcggataaag aagaataaaa actggaggaa taaattttac agataaaggc
     5281 ttagaaggag tcacgatgat taataaaaag gtagtattac cttaggcatt gagtaaggaa
     5341 t
//
LOCUS       DACEEZ010000133         5088 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20313_2017
            SAMN12639966-rid9879223.denovo.137, whole genome shotgun sequence.
ACCESSION   DACEEZ010000133 DACEEZ010000000
VERSION     DACEEZ010000133.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639966
            Sequence Read Archive: SRR10023158
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5088)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5088)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741683.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741683.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:44:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,016
            CDSs (total)                      :: 2,962
            Genes (coding)                    :: 2,889
            CDSs (with protein)               :: 2,889
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 36 of 73
            Pseudo Genes (incomplete)         :: 29 of 73
            Pseudo Genes (internal stop)      :: 18 of 73
            Pseudo Genes (multiple problems)  :: 8 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5088
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639966-rid9879223.denovo.137"
                     /isolate="E20313_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(205..660)
                     /locus_tag="IWO89_002703"
     CDS             complement(205..660)
                     /locus_tag="IWO89_002703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0457395.1"
                     /translation="MKKQLFKTFLLSTSILTFGSAITPSGAVFANENNISDIATYSDD
                     EYYYVLDEPVIQTRTWSKPQSWSIAKGSRTYLGIETHSRGTKLSVSANVFGMKIEIAR
                     NTSASGKFKKYRQNARITVTYRVYRKVDNKYIRTQTSTANTQYIDYIPA"
     gene            complement(1269..1685)
                     /locus_tag="IWO89_002704"
     CDS             complement(1269..1685)
                     /locus_tag="IWO89_002704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001232859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0457396.1"
                     /translation="MRKTSVQKHVTLYPSHVDVLDSIIEKNPDVNNYSEAIRFLCMSY
                     EPDTNTITTSNQAKLNAMGKEISIMTEIMAEFADLYLKDTGVLIGTNSKVYQEVKEKI
                     ENQLNPSRRNQPKNRQKETNQTFTSGNFDPTNFLKR"
     gene            complement(1687..1968)
                     /locus_tag="IWO89_002705"
     CDS             complement(1687..1968)
                     /locus_tag="IWO89_002705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0457397.1"
                     /translation="MDILVRNINPKIIARIDKRCQELTTKTGKKWSRNKYLKVLIESD
                     FDRQLMNYKKDQFDLLLEKFIAIQTENTNILNDYVHTNNQLIETLIGGI"
     gene            complement(2415..3383)
                     /locus_tag="IWO89_002706"
     CDS             complement(2415..3383)
                     /locus_tag="IWO89_002706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016186668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HAQ0457398.1"
                     /translation="MSNEIVKYHHELNTIPLRKFTPVEMNLFFSIVSRMRDVGDKKVQ
                     FTFEQLKDLSNYKATANVRFIDDLETTYDKLMDLRFGRRSADGLQRERFVLFNQFKID
                     GKSDIPFAEIQVHEKALPLLNNLEEWVRYSLQQFNELESSYSKTMFRLLKQYRTKGFA
                     YFSKEDFHELLDIPKSYNQGTIDQRVFNPIRQELTAIFKGLTIKKKYGKGRGKPVIGY
                     QFTFKPEIKKSDDFNKHAPTKEEQNKALELANQQVQSEKAPTSLQEKIKEQKRKLELL
                     QQLEKLEREQNTQNKENNAQKSEINSDIPSELKKDLLDNLQNLFKD"
     gene            complement(4724..>5088)
                     /locus_tag="IWO89_002707"
     CDS             complement(4724..>5088)
                     /locus_tag="IWO89_002707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HAQ0457399.1"
                     /translation="FSVLFLINILNTSFSEGINPNEVNGSLLLFFLNAATYGAFLEEG
                     IFRFCMIDPQANKKQQYISILISSFLFSIVHGGGLSIFFIGIILSFVYIQTKNIWYSI
                     VAHGFYNTIGILIYLISI"
BASE COUNT         1594 a          839 c          839 g         1816 t
ORIGIN      
        1 aaaaaaaact atatttaaag agttttttta gatttacttc tttgaattca cgaatgaagg
       61 tagaatgaaa tagtataatt attagcacta aaaaaaatat atcactatgt tcaaataaaa
      121 aatatatatt tgacggtact cctaatgatg ggatagcaaa ggtcaaaacc aagtaagcaa
      181 tactaattat ccattttatt gtcattaggc aggaatataa tcaatatatt gtgtatttgc
      241 tgtagaagtt tgagtacgaa tatatttatt atctacctta cgataaactc tgtaggttac
      301 agttattctt gcattttgac gatatttttt aaattttcca cttgcagaag tgtttctggc
      361 tatttcgatt ttcatcccaa atacatttgc actgacagat aactttgtac ctcgactatg
      421 agtttctata cctaaataag tacggcttcc tttagcaatt gaccagcttt gaggtttaga
      481 ccaagtcctt gtttgaataa ctggttcgtc taacacatag tagtactcat catcagaata
      541 agtggctata tcagaaatgt tgttctcatt agcgaatacc gctccgctag gagtaatagc
      601 agaaccaaaa gttaaaatag aagtagataa taaaaaggtt ttaaataact gttttttcat
      661 caaaatcacc tcgaatataa gatacttaag aaaacgcttt ctttatataa ttagaataat
      721 caggaaattt aatgttgtca agagggttta ttttataaaa gctgttatat taaaggttag
      781 ttgacataat gtacactatg ccaagtagcc cctctaccaa tggatagaga cgctcacttt
      841 gtgatttcta attggtctag cttaaacact tcagccaatg cttgatttta tgcacttttc
      901 ttttttcatt tctttgtgca ttttgaccgg tcatttttta gagatttgtt atttttgtga
      961 gcatttgata tgctcttttt atacctcaat ctcttctttt ctaatgttaa tgaataggat
     1021 aatctcattt ttagctgctc gtgagagcag tctttttttg tttcagggta cgtcctattc
     1081 tttggatttc cgcgctatta aataaccgat acttggggat tggggtagcg ccccaaaata
     1141 caaaaagaag aagttgcata agcggatttt tctttttttt cgcaagttgg taccaacttg
     1201 caaatcttgc tggaagactg atccctaaaa agaaatcgag gaattttttt agggatcggt
     1261 ttcttttttt atctttttag aaagtttgtt gggtcaaaat ttccagaagt aaaggtttga
     1321 ttggtttctt tttgacgatt tttgggttga tttcgtcggg aaggattgag ttgattttca
     1381 attttttctt tcacttcttg atacacttta gaatttgtac cgataagaac accagtatct
     1441 tttagatata aatcagcaaa ttctgccatg atttctgtca tgatagaaat ttcttttccc
     1501 atggcattga gttttgcttg gttactggtt gtgattgtat tggtatcagg ttcataactc
     1561 atacataaaa aacgaatggc ttctgaataa ttgttgacgt ctggattttt ttcgatgatg
     1621 gaatctaaaa cgtcaacgtg actgggataa agggttacgt gtttttgaac agaagttttt
     1681 ctcatgttat attcctccga taagtgtttc aatgagttgg ttgtttgtgt gaacatagtc
     1741 attcagtatg ttcgtatttt ctgtttgaat ggcaataaat ttttcgagta ataaatcgaa
     1801 ttgatctttt ttgtaattca taagttgacg gtcaaaatca ctttcgatta gaacttttag
     1861 atatttattt cgactccatt ttttgcccgt tttggttgtg agttcctgac aacgtttatc
     1921 tattcttgca ataattttgg gattgatatt tcgaactaaa atgtccatgg aaattttcct
     1981 ttcgtatcag cgtataattt atgtatatca tacaaatata gtatttgtat agctattttt
     2041 cgccaaaaca ctatacatag cctagccaca atggcaaaaa agtgatagca aatttttttc
     2101 tgagaaacgt gataggcaca gcctatctag caccttttgt aagaaagtga taccacgttt
     2161 ccttatgctc tttcaaaatt cagaaaaatc agctgtaaaa cgagtgcagt ccgagtaaga
     2221 ttcaacgaaa aaacggggat tggggcgcca ccaaaatatc gcaacatggg tccatgttgc
     2281 tctgctcgca aaagcaaaaa gaagaataaa accagcaatt taaattaata aaaattgctg
     2341 gttttattgc cagaattgcc accatttttt ctccgtcttt gattgttttt tctgtcataa
     2401 aatctaaaaa cccctcaatc tttgaataag ttttgaagat tgtctaataa gtcttttttt
     2461 agttctgaag gaatatcgga atttatctct gatttttgtg cattattttc tttattttgg
     2521 gtattttgtt ctctttctaa cttttctaac tgttgtaaaa gttccaattt tcgcttttgt
     2581 tctttgattt tttcttgtaa tgaagtaggt gctttttcag attgaacttg ttggttagct
     2641 agttctaatg ctttgttttg ttcttctttt gttggggcat gtttgttaaa gtcgtctgat
     2701 ttttttatct caggtttaaa tgtaaattga tagccaatga caggctttcc ccgtcctttt
     2761 ccgtatttct ttttgattgt taaaccttta aagattgcag ttagttcttg tcgtattgga
     2821 ttgaacacac gttgatcaat cgtaccttgg ttgtaacttt taggaatatc aagtaattca
     2881 tgaaagtctt cttttgaaaa ataagcgaaa cctttggttc tgtattgttt tagtaatcga
     2941 aacattgttt ttgaataact gctttctaat tcattgaatt gttggagaga atagcgcacc
     3001 cattcttcaa gattattaag taagggtaag gctttttcat gaacttgtat ttcagcaaaa
     3061 ggaatatcag attttccgtc gattttaaat tgattaaata aaacaaaacg ttcacgttgc
     3121 aaaccgtcag cacttcttct accaaaacgt aaatccatta atttgtcata cgttgtttct
     3181 aaatcatcta tgaatcgaac atttgcagtt gctttataat tgcttaaatc ttttaattgc
     3241 tcgaatgtaa attgtacttt tttatcacct acatcacgca ttcttgatac gatagaaaag
     3301 aacaaattca tttcgactgg tgtaaatttt cgcaacggaa ttgtatttaa ttcatgatga
     3361 tactttacga tttcattaga catatttaaa atagctcctt tcttactcgc acaagagata
     3421 cacgttatga aatacaaaat gcctatttat ctacatctta tattaacatc gtcgatgtac
     3481 taattcaatg tacaaacaga caaaacgatg tagataaaca gacaaaacga tgtagataaa
     3541 cagacaaaac gatgtagata aacagacaaa acgatgtaga taaaagcgtc attcccttgg
     3601 gagagtaagg atatagccga cggtaaagag gtttaaaaag aggtttaaaa agagattata
     3661 aaagaggggg acaaaatttc ccgcctaaaa aaccacttct tttttctcca aacagattcg
     3721 cgcatttttc tcaactggga atcttactcc ctacagtcgc ttaaaaaggt gtgatcgctc
     3781 tgctctcaca ttatactgcg cagtcgcttc cggctccttt gctaaaaata ttcataagga
     3841 actctttgag aagctatgag ccttcgtttg agacgtttta agacagtctt agtataaatc
     3901 ccttatcttg ttcttaaagc gccttacagg cggtcagggc aagccctaac aaccctaaag
     3961 ttgtctgaat gccctctaaa attcgattta agcactctta aacctattct tatatattta
     4021 tatacttaga cgtttcaaac gtcttaaaac gcaaattaga gcttgataaa gatatttgct
     4081 cgtgtgccga cttttttaaa agtctaggca cacgccatga caaagctgaa ataaactgct
     4141 tcagctttgt catggcggat tcgctcactt gaaaaagtgt tcatttcgct cgattattag
     4201 catatatttt ttaacttttt ttgctagcca aattttgcat tagttcgctc ataaatccca
     4261 ccctttcagt acgtcctacg gacgtatcgt tcgtaatgtg aaagtacctt taaaggtact
     4321 ttctacaaaa ctcactaaag ggtttggcaa gctctatatt tgctagccaa atacctacgt
     4381 atttggtgca aataaccgct tgtcaaaggg gcgcaagctc ccccttgaat cccccctcaa
     4441 aacccacgct ccacaataca aaaaaggcta gcaaatgcta gccacttttt gtaaagtttc
     4501 gcttgtttca ccttcggtga aattgttgct ttgcaacgat caggtagagc tacatttttt
     4561 ttcgttgttc aaatattctt acgccattta ctattaaaat cgtaggtatt ccaaagtaaa
     4621 gaaaaaattg attagcaggt aatacaaaat ataaaataat accagcaata attaccaaac
     4681 ttatagtaag ttttgttaat ttcatgaagt atgccacctt atcttatata gatatcaaat
     4741 agattaatat gccgattgta ttataaaatc catgagcaac tatggaatac catatatttt
     4801 ttgtttgtat ataaacaaaa cttaaaataa taccaataaa gaaaatactt aggcctcccc
     4861 catgaacaat agaaaacaga aaagaagaaa ttagtattga aatatattgt tgttttttgt
     4921 ttgcttgagg atcaatcata caaaatcgaa aaattccttc ttctaaaaaa gctccataag
     4981 tagcagcatt taaaaagaat aaaaggagag aaccatttac ttcattagga tttatacctt
     5041 ctgaaaaaga tgtatttaga atattgatta ggaataaaac agagaatg
//
LOCUS       DACEEZ010000134         4788 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20313_2017
            SAMN12639966-rid9879223.denovo.138, whole genome shotgun sequence.
ACCESSION   DACEEZ010000134 DACEEZ010000000
VERSION     DACEEZ010000134.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639966
            Sequence Read Archive: SRR10023158
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4788)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4788)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741683.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741683.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:44:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,016
            CDSs (total)                      :: 2,962
            Genes (coding)                    :: 2,889
            CDSs (with protein)               :: 2,889
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 36 of 73
            Pseudo Genes (incomplete)         :: 29 of 73
            Pseudo Genes (internal stop)      :: 18 of 73
            Pseudo Genes (multiple problems)  :: 8 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4788
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639966-rid9879223.denovo.138"
                     /isolate="E20313_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            564..989
                     /locus_tag="IWO89_002708"
     CDS             564..989
                     /locus_tag="IWO89_002708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0457400.1"
                     /translation="MICKNKKKIIAIIASFSLIFSTACTVLLTNNANVEAASSSYKHN
                     HAGYRCYKMSYYVSPSKCKQLARQTNKLNTVSAIANFIGLSGLTPGIISLVFGSAVSA
                     NNVFVTAARRGKGVQLNYIAHFSNTTTYNYSSNQNYVIK"
     gene            2141..3118
                     /locus_tag="IWO89_002709"
     CDS             2141..3118
                     /locus_tag="IWO89_002709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016186668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HAQ0457401.1"
                     /translation="MANEIVKYHHELNTIPLRKFTSVEMNLFFSIVSRMRDVGDKKVQ
                     FTFEQLKDLSNYKATANVRFIDDLETTYDKLMDLRFGRRSADGLQRERFVLFNQFKID
                     GKADIPFAEIQVHEKALPLLNNLEEWVRYSLQQFNELESSYSKTMFRLLKQYRTKGIA
                     YFSKEDFHELLDIPKSYRESDINKKIIKPIRQELTAIFKGLTIKKKYGKGRGKPVIGY
                     QFTFKPEIKKADDFNKHAPTKEEQNKALELVNQQIYSEKDPTSLQEKIKEQKRKLELL
                     QQLEELERKQNNLNKENKEINEQIPDNNSDIPPELKKGLLDNLQNLFKE"
     gene            3128..3625
                     /locus_tag="IWO89_002710"
     CDS             3128..3625
                     /locus_tag="IWO89_002710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAQ0457402.1"
                     /translation="MTDKTIKELADILGVSKDKVKYQLRKLPSNFTYKKGNIIYLKKE
                     AVTTITKVLSGNEPGNLPSELPDSLPTDYLLKEIETKNNHIEEKDRQIEKLQLALETQ
                     QKLLDQQQQLTLQANQQIEKLQEQLQLTYTEESSENKSTTLSEETESIEKQSKTEKKW
                     WQFWQ"
     gene            4001..4282
                     /locus_tag="IWO89_002711"
     CDS             4001..4282
                     /locus_tag="IWO89_002711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0457403.1"
                     /translation="MDILVRNINPKIITRIDKRCQELTTKTGKKWSRNQYLKILIEND
                     FDHELMNYKKDQFDLLFEKFIAIQAENTNILNDYVHTNNQLIETLIGGI"
     gene            4284..4700
                     /locus_tag="IWO89_002712"
     CDS             4284..4700
                     /locus_tag="IWO89_002712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001232859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0457404.1"
                     /translation="MRKTSVQKHVTLYPSHVDVLDSIIEKNPDVNNYSEAIRFLCMSY
                     EPATNTTTTSNQAKLNAMGKEISIMTEIMAEFADLYLKDTGVLIGTNSKVYQEVKEKI
                     ENQLNPSRRNQPKNRQKETNQTFTSGNFDPTNFLKR"
BASE COUNT         1757 a          816 c          786 g         1429 t
ORIGIN      
        1 aaaaaaaaaa gaaaaatccg cttctgcgaa ttcttctttt ttcattttgg ggcgctgccc
       61 taatccctaa gtcccggtta ttcaatagca tggaaactca aagaataaga cgttccctgg
      121 aataaaaaaa aggctgctcg ttcgagcagt ccatacatag gaaaatgaaa aaacgacgtc
      181 ccttactgag aaacgctatt aaacttttaa caaatcctct aaaaaataac aagtctttaa
      241 aaagtgaccg gtaaaaatgc acaaagaact aaaaaagaga aattgcatca aatcaagcat
      301 tagaagctag taactagcta gaccaattga aaatcacaaa gtgagcgcct gtatctattg
      361 ctacaggcgc tacttggcat agtgtacatt atgtcaacaa gaatttaata taacatattt
      421 ttctagtttg ttataacaat gccactctaa caaaaatgta agcattatcc ttattaaaag
      481 ctccttgaca aggactatac cttttatttt atgattttta catagaaaac gttttctcta
      541 ttttttgttt agaggtgatt tttatgattt gcaagaacaa gaaaaaaatt attgctatta
      601 ttgctagttt ctctttgatt tttagtactg catgtactgt cttattaaca aataatgcta
      661 atgttgaagc tgcaagctct tcttacaaac ataatcatgc tggttataga tgttacaaaa
      721 tgagttatta tgtaagtcct tctaaatgca aacaattggc tcgccaaact aataaactta
      781 ataccgtctc agctattgca aactttattg gactatctgg attaacaccg gggattatat
      841 cattagtttt cggatcagca gttagtgcta ataatgtatt tgttacagcc gctagaagag
      901 gaaaaggcgt tcaattaaat tatattgccc attttagtaa taccactacc tataattata
      961 gttctaacca aaattatgtt ataaaataat taaaacttta aactaggagg aatgctttat
     1021 gaaattaaca aaaattgctg taattttagc tgttattgct ggcattattt tatattttgt
     1081 attgcctgct aatcaattct tcctttactt tggaatacct gcgattttaa tagtaaatgg
     1141 cgtaagaatc tttgaacaac gaaaaaaatg tagctctacc taatcgttac aaaacaacaa
     1201 tttcaccttc ggtgaaataa gcgaaacttt acaaaaagtg gctagcaaat gctagccttt
     1261 tttgtattgt agagcgtgaa ttttgagggg gattcaaggg ggagcttgcg cccctttgac
     1321 aagcggttat ttgcaccaaa tacctccgta tttggctagc aatccttcta aaaaatacac
     1381 ctatcgatca gagtcaaaac aaatcaattt tcgagtgagc gaatccgaat ttgcaaagct
     1441 gaagctttgc aaatgagtgt gccggcttcc gtaaaaatac atatggctat aatttgcgtt
     1501 ttaaggcgtt ttaaacgttc tggtatataa ttataccaaa acaactttaa aacgccttag
     1561 atctaatttt agaggtctta tagaacattt tagggttgtt agggcttgcc ctgaccgcct
     1621 gtaaggcgct ttgagaacaa gataagggtt ttatactaag actgtcttaa aacggcttaa
     1681 acaaacgctc agaacttttc aaaggagttc tttatgaact gtttttaagc aaaggagcct
     1741 ttaggcgact gcgcagtata atgtgagagc gtagcgatca cacattttta gtgaccgtag
     1801 ggagcgagtt tcccagttta gaaaaattcg cgaacttgtt tgaggaaaaa agcaggagtt
     1861 ttttaggcgg gaaattttgc ctcctctttt ataatctctt tttaaacctc tttttaaacc
     1921 tctttaccgt cggctgtatg cttactctcc caaggtgtgc agaatctttt gggggtcttt
     1981 ttgtctgttt ttaggggtct ttttgtctgt ttttaggggt ctttttgtct gtttttgggg
     2041 gtctttttgt ctgtttttag gggtctttaa ttaggggctt gacccctatt agaaagctct
     2101 ttataatttt gttaagtagt taatcttgaa ggaggatttt atggcaaatg aaattgtgaa
     2161 ataccaccat gaattaaata cgattccttt acgcaaattt acgtcagtag aaatgaattt
     2221 attcttctct atagtatcta gaatgcgtga cgtaggcgat aaaaaagtac aatttacatt
     2281 cgaacaatta aaagatttaa gcaattataa agcaactgca aatgttcgat tcatagatga
     2341 tttagaaaca acgtatgaca aattaatgga tttacgtttt ggtagaagaa gtgctgacgg
     2401 tttgcaacgt gaacgatttg ttttatttaa tcaatttaaa atcgatggaa aagctgatat
     2461 tccttttgct gaaatacaag tccatgagaa agccttaccc ttacttaata atcttgaaga
     2521 atgggttcga tattctctcc aacaattcaa tgaattagaa agcagttatt ccaaaaccat
     2581 gtttagatta ttaaaacaat acagaaccaa aggaattgcc tatttttcaa aagaagactt
     2641 tcatgaatta cttgatatcc ctaaaagtta tagagaaagt gatataaata aaaaaattat
     2701 aaagcctatt cgccaagaac taaccgcaat cttcaaaggt ctgacaatca aaaagaaata
     2761 cggaaaagga agaggaaaac ctgtcattgg ctatcaattc acgtttaaac ctgaaataaa
     2821 aaaagcagac gactttaata aacacgcacc aacaaaagaa gaacaaaata aagctttaga
     2881 actagttaat caacaaattt actctgaaaa agaccccact tcattacaag aaaaaatcaa
     2941 agaacagaaa cgaaaattag aacttttaca acaattagaa gagctagaaa gaaaacaaaa
     3001 taatctgaat aaagaaaata aggagattaa cgaacaaata ccagataaca attccgatat
     3061 tcccccagaa ttaaaaaaag gcttactaga taatcttcaa aacttattta aagaataagg
     3121 ggtttttatg acagataaaa ctatcaaaga attagcagat attctaggtg tttcaaaaga
     3181 taaagtgaag tatcaactga ggaaattacc tagtaatttt acctacaaaa aaggaaatat
     3241 tatctacctg aaaaaagaag cagtaacaac aattacaaag gttttatcag gtaatgagcc
     3301 gggtaactta cctagtgaat tacctgattc attacctacc gattacctac tcaaggagat
     3361 tgaaactaag aataatcata ttgaagaaaa agataggcaa attgaaaaac ttcaactagc
     3421 tttagaaaca caacaaaaat tactcgacca gcaacaacaa ctcaccctgc aagccaacca
     3481 acaaatcgag aaactgcagg aacaactaca gttgacttat acggaagaat cttcggaaaa
     3541 taaatcaacg actctttctg aagaaacaga atcgatagaa aaacaatcaa agacggagaa
     3601 aaaatggtgg caattctggc aataaaacca gcaatttaaa ttaataaaaa ttgctggttt
     3661 tattcttctt tttgcttttg cgagcagagc aacatgggcc catgttgcga tattttggtg
     3721 gcgccccaat ccccgttttt tcgttgaatc ttactcggac tgcactcgtt ttacagctga
     3781 tttttctgaa ttttgaaaga gcataaggaa acgtggtatc actttcttac gaaaggtgct
     3841 agataagctg tgcctatcac gtttctcaga aaaaaatttg ctatcacttt tttgccattg
     3901 tggctaggct atgtatagtg ttttggcgaa aaatagctat acaaatacta tatttgtatg
     3961 atatacataa attatatact gatacgaaag gaaaatttcc atggacattt tagttcgaaa
     4021 tatcaatccc aaaattatta caagaataga taaacgttgt caggaactaa caaccaaaac
     4081 gggcaaaaaa tggagtcgaa atcaatacct aaaaatttta atcgaaaacg attttgacca
     4141 cgaacttatg aattacaaaa aagatcaatt cgatttatta ttcgaaaaat ttattgccat
     4201 tcaagcagaa aatacgaaca tactaaatga ctatgttcac acaaacaacc aactcattga
     4261 aacacttatc ggaggaatat aacatgagaa aaacttctgt tcaaaaacac gtaacccttt
     4321 atcccagtca cgttgacgtt ttagattcca tcatcgaaaa aaatccagac gtcaacaatt
     4381 attcagaagc cattcgcttt ttatgtatga gttatgaacc tgctaccaat acaaccacaa
     4441 ccagtaacca agcaaaactc aatgccatgg gaaaagaaat ttctatcatg acagaaatca
     4501 tggcagaatt tgctgattta tatctaaaag atactggtgt tcttatcggt acaaattcta
     4561 aagtatatca agaagtgaaa gaaaaaattg aaaatcaact caatccttcc cgacgaaatc
     4621 aacccaaaaa tcgtcaaaaa gaaaccaatc aaacctttac ttctggaaat tttgacccaa
     4681 caaactttct aaaaagataa aaaaagaaac cgaaaaataa aaaaattcct cgatttcttt
     4741 ttagggatcg gtcttccagc aagatttgca agttggtacc aacttgcg
//
LOCUS       DACEEZ010000135         4720 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20313_2017
            SAMN12639966-rid9879223.denovo.140, whole genome shotgun sequence.
ACCESSION   DACEEZ010000135 DACEEZ010000000
VERSION     DACEEZ010000135.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639966
            Sequence Read Archive: SRR10023158
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4720)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4720)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741683.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741683.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:44:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,016
            CDSs (total)                      :: 2,962
            Genes (coding)                    :: 2,889
            CDSs (with protein)               :: 2,889
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 36 of 73
            Pseudo Genes (incomplete)         :: 29 of 73
            Pseudo Genes (internal stop)      :: 18 of 73
            Pseudo Genes (multiple problems)  :: 8 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4720
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639966-rid9879223.denovo.140"
                     /isolate="E20313_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(<1..1097)
                     /locus_tag="IWO89_002713"
     CDS             complement(<1..1097)
                     /locus_tag="IWO89_002713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016180804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="HAQ0457405.1"
                     /translation="MARKKRNPDAEKLAESILNTYQPESVEDMQDVLKDVFGPLFEKM
                     LQGELNNHLGYDAHSKEPKKHDNRRNGYGNKTLKTSFGKVNIDVPRDREASFEPELIP
                     NRKRDVSDIEGKVLSMYARGMSQCDIAATVEDIYGFDISHEMISDITDAVLPELEEWQ
                     ARPLAKCYAFLFVDCMYVTLRENYEAKEYAVYTILGYDLKGNKEILGLWLNQTESKNR
                     WMQVFDELKVGGVEDVFFISMDGVSGLKEGAKAIFPSVIVQRCIVHLVRNALRYLPSK
                     DYKEVCRDMKKFYSASSLNAAHAAFDSFQNRWSHYSGAVDVCKRNFSHVEQLFDYGSA
                     IRKIMYTTNAVESIHSSFRKVTKKGVFSNENA"
     gene            complement(1227..2603)
                     /locus_tag="IWO89_002714"
     CDS             complement(1227..2603)
                     /locus_tag="IWO89_002714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017647836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAQ0457406.1"
                     /translation="MIKNAFAYVTRKSLKSLIIILVILSMSTLSLISLSIKDATDRAS
                     KETFANITNSFSMEINRQVNPGTPRGGGNVKGEDIKKISQTDSIDSYVKRINSVADLV
                     DYDIIETQETLANQSPERAKNFKRTVMLTGVNDSTKETKFVSEAYKLVEGKHLENEDK
                     NKILMHKDLAEKNNLKVGDKIKIKSNLFDADNEKGADETVEVEIKGLFDGHNSGGVSA
                     AQELYENTLITDIHTAAKVYGNTEDTAVYQDATFFVKGDKNLDSVIKDLGKLDINWRE
                     YNLIKSSSNYPALQQSISGIYSISNKLFFGSLIFAGVVVSLLLFLWMNARKKEIAVLL
                     SLGISKLEIFGQFLIEMVFISIPAFVGSYFLAQYTADKLGNNILNKVTGDIAKQIAKQ
                     SASSQLGGGAEAEGFNKTLSSLDINVLPKFMIYVVIFMSLVLLVSLIISSFNILRRNP
                     KELLIDNN"
     gene            complement(2603..3259)
                     /locus_tag="IWO89_002715"
     CDS             complement(2603..3259)
                     /locus_tag="IWO89_002715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004612033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ0457407.1"
                     /translation="MDILEIKNVAYSYANSKEKVLSGVNQKFELGKFYAIVGKSGTGK
                     STLLSLLAGLDKPQTGKILFKNEDIQNKGYSNHRKNNISLVFQNYNLIDYLSPIENIR
                     LVNKSADESILFELGLDKKQIKRNVMKLSGGQQQRVAIARALVSDAPIILADEPTGNL
                     DSVTAGEIINILKTLAKDRNKCVIVVTHSKEVADSADIILELSGKKLKKVNKMNLEVE
                     "
     gene            complement(3269..4546)
                     /locus_tag="IWO89_002716"
     CDS             complement(3269..4546)
                     /locus_tag="IWO89_002716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAQ0457408.1"
                     /translation="MIKNAIAYITRKKNRTLIIFIILTIVLSCLYSCLTIMKSSNEIE
                     KALYESSNSSISITKKDGKYFNVNQFKDIEKLKEIEEKIIQYDGLAKLKDAKVVSGEQ
                     RINREDLSDEFKNVVSLEATNNTKRNILFSSGVFTIKEGKNIEENDKNSIIVHEEFAK
                     QNNLKLGDEVDLELLDIEKSGKIKSHKFKIIGIFSGKKQETYTGLSSDFSENMVFVDY
                     STSQEILNNSENNEIANKILMYSGSAESTDLALNKLKELKIDESKYFVEKDSNAFEES
                     LESVSGIKYIIKVMTYSIMLGGMVVLSLILILWLRERIYEIGIFLSIGTSKIQIIMQF
                     IFELIFISIPSIISSLFLGNVLLKVIVDGFINSEDSMISGGSLINNSSFMSNITTLGQ
                     SYLILISIIVLSVVFASSLMLIKKPKEILSKIS"
     gene            complement(4565..>4720)
                     /locus_tag="IWO89_002717"
     CDS             complement(4565..>4720)
                     /locus_tag="IWO89_002717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008392734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(4Fe-4S)-binding protein"
                     /protein_id="HAQ0457409.1"
                     /translation="DKDKCINCGKCKKVCQMNKDSVENCNHLECIRCGRCKNACPVDA
                     ISYGVRK"
BASE COUNT         1477 a          858 c          566 g         1819 t
ORIGIN      
        1 gcattctcat tagagaacac tccctttttt gtgacttttc tgaagctaga gtgaatactt
       61 tcaactgcat tagtagtgta catgattttt ctaatggcgc tgccataatc aaagagttgt
      121 tcaacatgag aaaagtttcg tttgcataca tctactgcac cagaataatg agaccaccga
      181 ttttgaaaac tgtcgaaggc agcgtgtgca gcgtttaaag aagaagcact gtagaatttt
      241 ttcatgtctc gacagacttc cttataatcc ttacttggga ggtatcgtaa cgcatttcga
      301 acaagatgaa caatacagcg ctgcacaatg acagaaggaa aaattgcttt tgcgccttct
      361 ttgagaccgg aaacaccgtc catcgaaatg aaaaaaacat cttctacacc acctactttc
      421 agttcatcaa atacttgcat ccagcgattt ttagattctg tttgattgag ccataggcct
      481 aaaatctcct tgtttccttt aagatcatag ccaagaatgg tgtacacagc atattcttta
      541 gcttcatagt tttcgcgtaa agtgacgtac atacaatcaa cgaatagaaa tgcataacac
      601 ttggctaatg gccgagcttg ccattcttcc aattcaggaa ggacggcatc cgtgatatca
      661 gaaatcattt cgtgggaaat atcaaagccg taaatatctt caacggttgc ggcgatatcg
      721 cattgactca ttcctcgtgc atacatggaa agaaccttac cttcgatgtc ggagacatct
      781 cgttttctat taggaatcaa ctcaggttca aaagaagcct cccggtctct gggaacatca
      841 atgtttactt taccaaagct cgttttaagc gtcttattgc cataaccgtt tcgtcgattg
      901 tcatgtttct ttggctcttt agaatgggcg tcatacccta aatggttatt caattctcct
      961 tgaagcattt tttcaaataa gggaccaaac acatctttta aaacatcttg catatcctct
     1021 acagattcgg gttgataagt attcaaaatg gattcagcta acttctcagc atcaggattt
     1081 cttttctttc tagccatcgt gtaatcacct cttgatctta ttttagaaaa ataaaaaccg
     1141 tgaagcaacc acccgcctag gattgatggt tacttacacg gtttatatta cactctcatt
     1201 tttggtgcta aaaagcaccg aaaattttaa ttattgtcaa ttaataattc ttttggattc
     1261 cttcttaata tattgaatga agaaataatc aatgatacaa gaagtactaa actcataaat
     1321 ataactacat aaatcatgaa tttaggtaat acattgatat ctaaacttga taatgtcttg
     1381 ttaaatcctt cagcttctgc cccaccacct aattggctag atgcagattg tttagctatt
     1441 tgtttagcta tatcaccagt aaccttattc aatatattat tcccaagctt gtcagctgta
     1501 tattgagcta agaaatagga accaacgaat gcggggattg atataaatac catctcaatt
     1561 aggaattgac caaaaatttc taattttgat atacctaatg atagtaatac tgctatttct
     1621 ttctttctag cattcatcca taagaataat aataatgaaa ctacaactcc tgcaaatatt
     1681 aatgagccaa aaaataattt atttgaaatt gagtaaatac ctgatataga ttgttgtaat
     1741 gcagggtaat ttgatgaact tttaatcaaa ttatattctc tccaatttat atctaatttc
     1801 ccaagatctt ttattacact atcaagattc ttatcacctt ttacaaagaa tgttgcatct
     1861 tggtatacag ctgtatcttc ggtattacca taaactttag cagctgtatg aatatcagtg
     1921 attaatgtat tttcgtatag ttcttgtgct gcacttactc caccgctatt atgcccatcg
     1981 aatagacctt taatttctac ttctactgtt tcatctgcac ctttctcatt atcagcatca
     2041 aataaattag attttatttt tatcttatct ccaactttaa ggttattttt ttcagctaaa
     2101 tctttatgca ttaagatttt atttttatct tcattttcta aatgctttcc ttctactaat
     2161 ttatatgctt ctgatacaaa ttttgtttct tttgttgagt cgttaacccc agttaacata
     2221 actgttcttt taaaattctt tgctctttca ggtgattgat tcgcaagagt ctcttgagtt
     2281 tcaataatat cataatcaac taaatctgca acactgttta ttctttttac ataggagtct
     2341 atactatctg tttgagatat ctttttaata tcttcacctt ttacatttcc cccacctcta
     2401 ggtgttcctg gatttacttg tctatttatc tccatagaaa aactatttgt tatattagca
     2461 aatgtttctt ttgaagctct gtccgtagca tctttaatag ataaactaat gagacttaaa
     2521 gttgacatag ataaaataac taatatgata attaatgatt ttaaactttt tctagttaca
     2581 taagcaaatg cattttttat cattattcaa cctccaaatt cattttattt acttttttca
     2641 actttttacc acttagttct aaaatgatat ccgcagaatc tgctacttcc ttactatgtg
     2701 ttacaactat tacacattta tttctatctt tagctaatgt ctttaatata ttaattattt
     2761 ctccagcagt aacactgtct agattaccag taggctcatc agctagtatt attggagcat
     2821 ctgataccaa tgctctagca atagctactc tttgttgctg tccaccagat aatttcataa
     2881 catttctttt tatttgtttt ttatctaaac ctagttcaaa caagatactt tcatctgctg
     2941 atttatttac taatctaata ttttcaattg gtgataaata atctattaaa ttatagtttt
     3001 gaaatactaa agatatatta ttttttctat gattactata tcctttattt tgtatatctt
     3061 cattcttaaa caatattttt cctgtttgag gtttatcaag tccagcaagt aaggaaagaa
     3121 gtgtagattt tcctgttcct gactttccta ctatcgcata aaacttccca agttcaaatt
     3181 tttgatttac tcctgacaaa actttttctt tagaatttgc ataactatat gctacattct
     3241 ttatttctaa tatatccatt attttctcct aacttatttt tgataagatt tctttaggct
     3301 tcttgattaa cattaatgaa gaagcaaata caactgataa aacaataata cttattaata
     3361 ttaaataact ttgtccaagt gttgttatat ttgacataaa actactatta tttattaaac
     3421 ttccaccgga aatcattgaa tcctctgagt taatgaatcc atctacaatt acttttagta
     3481 atacatttcc taaaaataag gaagatatta tacttggtat cgatatgaat attaactcga
     3541 atataaattg cattataatt tgtatcttag atgttccaat agataagaat atacctattt
     3601 cataaattct ttcccttaac catagaatca agattaatga aagaacaacc atcccaccta
     3661 acataataga ataagtcatt acttttatta tatattttat tccactcact gactctaaag
     3721 actcttcaaa cgcattagaa tctttttcaa caaaatactt tgactcatca attttaagct
     3781 ctttcaattt gtttaaggct aagtctgtag attctgcact accagaatac attaaaattt
     3841 tatttgcaat ttcattattt tctgaattat ttaatatctc ttggcttgtt gaataatcta
     3901 caaacaccat attttcacta aaatcagaag ataatcctgt atatgtttcc tgttttttac
     3961 cagaaaagat ccctataatt ttaaatttat gactttttat ttttccactt ttttcaatat
     4021 ctagtaattc aagatcaact tcatcaccta attttagatt gttttgttta gcaaattctt
     4081 catgaacaat aattgaattc ttatcatttt cttctatatt tttaccttct ttaattgtaa
     4141 atactccact actaaataaa atatttcttt tagtattatt tgtagcttcg agtgaaacaa
     4201 cattcttaaa ttcgtcagat aaatcttctc tatttattct ttgttctccg ctaactactt
     4261 tagcatcttt tagttttgct aatccatcat attgaattat tttttcttca atttctttta
     4321 atttttcaat atctttaaat tgattaacat taaaatattt accatctttt tttgttattg
     4381 atattgaaga attagaactt tcatataaag ccttttctat ttcattgctt gatttcatta
     4441 ttgttaaaca agaatacaag caagaaagaa ctatagttaa tatgataaaa attattaaag
     4501 ttctattttt ctttcttgta atatatgcta tagcattttt tatcactttc ctaactactc
     4561 ctctctattt tcttactcca taactaattg catccactgg acaagcattt ttacatcgtc
     4621 cacatctgat acactccaga tgattgcaat tctcaactga atctttattc atttggcaaa
     4681 cttttttaca ttttccacaa ttaatacact tatccttatc
//
LOCUS       DACEEZ010000136         4632 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20313_2017
            SAMN12639966-rid9879223.denovo.141, whole genome shotgun sequence.
ACCESSION   DACEEZ010000136 DACEEZ010000000
VERSION     DACEEZ010000136.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639966
            Sequence Read Archive: SRR10023158
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4632)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4632)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741683.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741683.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:44:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,016
            CDSs (total)                      :: 2,962
            Genes (coding)                    :: 2,889
            CDSs (with protein)               :: 2,889
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 36 of 73
            Pseudo Genes (incomplete)         :: 29 of 73
            Pseudo Genes (internal stop)      :: 18 of 73
            Pseudo Genes (multiple problems)  :: 8 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4632
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639966-rid9879223.denovo.141"
                     /isolate="E20313_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            174..518
                     /locus_tag="IWO89_002718"
     CDS             174..518
                     /locus_tag="IWO89_002718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HAQ0457410.1"
                     /translation="MAEKQTAGRDQLGDFAPKFAELNDDVLFGQVWSREELSAHQRSI
                     ITISALISGGNFEQLPAHLKIGKQNGITSEEIAEIITHLAFYVGWPKAWSAFSLAKEI
                     YLEENSEDKKEA"
     gene            519..938
                     /locus_tag="IWO89_002719"
     CDS             519..938
                     /locus_tag="IWO89_002719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HAQ0457411.1"
                     /translation="MTTKHEEIHNGVIFPAGEKNEAYAQVFTGQSYLNMLVNDPEVSV
                     GVGNVIFEPGCRNHWHVHKDGYQILLVTGGEGWYQEEGKPARRLMAGDVVVTHKGIKH
                     WHGATKDSWFEHLAITAGNSEFLEPVTDEVYEGLENQ"
     gene            complement(1030..1380)
                     /locus_tag="IWO89_002720"
     CDS             complement(1030..1380)
                     /locus_tag="IWO89_002720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0457412.1"
                     /translation="MEKELSKRIIDELLTYLYSHEITEIRIGVEFAEEGLFIEIQGTA
                     ETQPEDLDVLADLLNNPRDPSMEIYYQELLGNPRHDREDYHLLGSLIDDVEIMYAQPI
                     INIKIFRRASHSYY"
     gene            complement(1346..2566)
                     /locus_tag="IWO89_002721"
     CDS             complement(1346..2566)
                     /locus_tag="IWO89_002721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1576 domain-containing protein"
                     /protein_id="HAQ0457413.1"
                     /translation="MLSNTPTDNWSGLLTILTSPSNLLTDYFALGGFGSAFINVGILT
                     LLSVLLAYRHKVILNGPLFASILTVTGFSFFGKNLYNSISIILGVYLYAVFVNKPFSQ
                     YIMIGLFGSALSPVVSYITFGMRFPLLIGILLGNLAGIAIGLLLPPLAAQTLVFHRGF
                     TLYNIGFTSGLIAMTFTAVLRLFSYSIVENTLVFNEYHFPLIWIIFGFFSLTVGIGFY
                     YNSFRLLGIREIFDSSGKLTTDFIANSGIGATLINIGLVGLMLSSYVLLVGGQLNGPV
                     IGAILSAVGFSAFGCHLKNSFPILVGIFIASLFGTFHEITSTGMLVAAVFGTGLAPIS
                     GFYGSFYGVIAGVLHIALVHNVSTLHGGLNLYNSGFSTGFVAGILVPILDNFTAVRKE
                     KKTLGKRIIKKNHR"
     gene            2932..3498
                     /locus_tag="IWO89_002722"
     CDS             2932..3498
                     /locus_tag="IWO89_002722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HAQ0457414.1"
                     /translation="MRRKVYTKDQILKAAYDVVAKEGFKGFTARNIAKKMGISTQPIY
                     LEFKNMEDLKNTLLDSIFEQLKEDVFPIERTGDKIIDLGLNYIYFAKKHHSLYMALYI
                     DEHGDGKKMHDFSYNYFREIVKEDERYNQLSDEYVDALHTGTWVAVTGLASLMSSNIM
                     HPTEEQIIALIKRTIDSILALDNPEIIR"
     gene            3643..3730
                     /locus_tag="IWO89_002723"
     tRNA            3643..3730
                     /locus_tag="IWO89_002723"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3675..3677,aa:Ser,seq:cga)
     gene            4064..>4632
                     /locus_tag="IWO89_002724"
     CDS             4064..>4632
                     /locus_tag="IWO89_002724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="linear amide C-N hydrolase"
                     /protein_id="HAQ0457415.1"
                     /translation="MCTAITYATKDHYFGRNFDYEMSYNEVVTITPRNYRFDFRKVKN
                     LDKHYAMIGIAAGVSNYPLYYEATNEKGLSMAGLNFPGNADYKELQEGKDNVAPFEFI
                     PWILGQCSTIDEAKELLATINLVNIDFSEKLPLSPLHWLLADKEKSIVIESMKDGLHL
                     YDNPVGVLTNNPPFDYQLFNLNNYRSLSNG"
BASE COUNT         1682 a          778 c          891 g         1281 t
ORIGIN      
        1 aaataaaaaa agcgttagca actatgctta aaaagcatat cgactgaata aactaagcat
       61 tggacaggct gaaagactac atttaaaatg aaatgcgaag aaacaataga aaataaagtt
      121 gccttagaac catagttgaa tcacctaaca gtcgtgaagg gagaataaga gaaatggcag
      181 aaaaacaaac cgcaggacga gatcaattag gagattttgc accaaaattt gcagaattga
      241 atgatgatgt tttatttgga caagtatggt ccagagaaga attatctgct catcagcgta
      301 gtatcatcac catatctgca ttaatttcag gaggaaattt tgaacaattg ccagctcatc
      361 tgaagattgg caagcaaaat ggcattacta gtgaagaaat agcagaaatc attacccatt
      421 tagcttttta tgtaggatgg ccaaaagctt ggtcagcttt tagtttagca aaagaaatct
      481 atttagaaga aaatagcgaa gataagaaag aggcgtaaat gacgacaaaa cacgaagaaa
      541 tacacaatgg cgtgattttt ccagcaggtg aaaaaaacga agcttacgct caagtattta
      601 ccgggcaaag ctatctgaat atgttagtga atgatcccga agtttctgtt ggtgtaggta
      661 atgtaatttt tgaaccaggt tgtcgcaatc actggcatgt ccataaagac ggctatcaaa
      721 tcttacttgt gaccggagga gaaggctggt atcaggaaga aggaaagcca gcaagacgat
      781 taatggctgg agatgtcgtc gtaacgcaca aagggattaa acattggcat ggtgcgacaa
      841 aagacagttg gtttgaacat ttggcgatca ctgctggtaa ttcagagttt ttagaacctg
      901 taacagatga agtgtatgaa ggattggaaa atcaataatg tcaagcttga acagctcctg
      961 aaagtagatt ttaatcgaac tttcaggagc tgcttttttg attcagtctt ttataaatag
     1021 aaaatagggt taataatagg aatgagaagc acgtcgaaag atcttaatat tgataatcgg
     1081 ctgggcatac atgatttcca cgtcatcaat caaactacct aacagatgat aatcttcccg
     1141 atcatgtcgg ggattaccta atagttcttg atagtagatt tccatactag ggtcccgagg
     1201 attgttcagc aaatcagcaa gtacatctaa atcttctggc tgagtttctg ccgtaccctg
     1261 tatctctatg aaaagccctt cctcagcaaa ttctactcca atacgtattt cagttatttc
     1321 atgggaatat aaataagtaa gaagctcatc gatgattctt tttgataatt ctttttccaa
     1381 gtgtcttttt ttcctttctg acagcggtaa agttatctag tatcggtaca aggatacctg
     1441 caacgaaacc agttgaaaaa ccactgttat atagatttag tcctccgtgc aaagtgctga
     1501 cgttatggac aagagcaata tgtagtacac cagctattac accgtaaaaa ctgccgtaaa
     1561 aaccgcttat gggcgcgaga cctgtcccaa acacagctgc aacaagcatg ccagtactgg
     1621 tgatctcatg aaaagttcca aacaaagaag caataaatat tccgactagt atcggaaaac
     1681 tgttttttaa atgacaacca aaagcactga aacccaccgc agataaaata gcaccgatga
     1741 caggtccatt taactgaccg ccaaccagca aaacataaga agacagcatc agaccaacta
     1801 agcctatgtt gatcaaagta gcaccaatac cagaattagc aatgaaatca gttgtcaatt
     1861 ttccagatga atcgaaaatt tctcgaatcc ccaaaagccg aaaggaatta taatagaaac
     1921 caattccgac tgtcagcgaa aaaaagccaa aaataatcca aatgagagga aagtgatatt
     1981 cattgaaaac caatgtattt tcgacgattg aatagctgaa taacctcaaa acagcagtaa
     2041 aagtcatcgc aatcaaccca gaggtgaatc caatattata aagtgtgaat ccacgatgga
     2101 aaactaaagt ttgagcagct aagggcggaa gaagtagacc aattgctatg ccagctaaat
     2161 tccccaataa aatacctatc agtaaaggaa aacgcatacc aaaagtgata taactaacga
     2221 ccggtgataa tgcgctacca aacagtccga tcatgatgta ctgagaaaaa ggtttattga
     2281 caaatacagc gtatagatac acacctaaaa tgattgagat agaattataa aggtttttgc
     2341 caaaaaaaga gaaaccggta actgttagaa tcgaagcaaa taagggaccg tttaaaatca
     2401 ccttgtgccg ataagctaaa agcacagaaa gtaaggtcaa aatccctaca tttatgaatg
     2461 cgcttccgaa accgcctaaa gcaaaataat ctgtcaaaag attgcttggc gaggtaagaa
     2521 tagttagcaa tccactccaa ttatcagtag gagtattaga aagcaagcca ataaaaagaa
     2581 aaaaacacct gtaaaaccaa gataaaaata ctttcttttc tgtgaaacga ttcgtttttg
     2641 aggatgaata taaataacgt cagtttcgtt ttccattaca caccttcttt ttcattttct
     2701 ttaactataa cttgaaatga aagatttgga caactatcat ttaggtcaaa actaaaaatg
     2761 taagggaacc cattgaattt aaccaaattt ttttaatttt gcaaaaaaaa gttaaagaag
     2821 actgaaaaaa aatttaaaat ataacgtttt gagtaaaatg tttgcaaaaa atgctaaaaa
     2881 tagggtacaa taaaacaaca tatactgcag taaagaagtg ggggagtttt aatgagaaga
     2941 aaagtctata ccaaggatca aattctaaaa gctgcttatg atgttgttgc aaaggaaggg
     3001 tttaaaggat ttactgcgag aaatattgct aaaaagatgg ggatttcgac acagccgatt
     3061 tatctggaat tcaaaaacat ggaagattta aaaaatacgt tgctggattc tatctttgaa
     3121 caactaaaag aagatgtttt ccctattgaa cgtactggtg ataaaatcat tgatctcggt
     3181 ttgaactata tctactttgc aaaaaagcat catagtcttt acatggcatt gtacattgat
     3241 gaacatgggg acggaaagaa aatgcatgat ttttcataca attatttccg tgaaatagtt
     3301 aaagaagatg aaagatacaa tcaattatcg gatgaatatg tcgatgctct gcatactggt
     3361 acatgggtag cagtgactgg cttagcgtca ttgatgtcat caaacatcat gcatccaaca
     3421 gaagaacaaa tcattgcact gatcaaacgg acaattgact cgattttagc attagataat
     3481 cctgaaataa tcagatagtg ttctatcagt atgaatttga tcggtaagtc cgttggctgc
     3541 aaactgtcaa tggacttttt tgctaaaaaa agcgatttct gttatttatc tattagaaat
     3601 aggcttgaca tttcgtgaaa aaagtttaaa aatagtactt acggaaggtt ggcagagtgg
     3661 taatgcaccg gactcgaaat ccggcgaacc ggctaatacc ggcgcgcagg ttcaaatcct
     3721 gtaccttcct tatatcaacg attagaggtt gtgaagaagc tgtcctgcat caagtaataa
     3781 gaacggccaa acaaaaatag cttttcctat tttttcgttt gattgggctt attaccgcag
     3841 atgcaagctt ttgaacaccg tttgttcgga ataaggggct gtgacaagac ttttgtcaca
     3901 gccccttatt gttgtacttt taacctacaa tattttttga ttccccctga gtactatttt
     3961 ttataaatgc tagtaaatca atcttctttg gccttttttt gataaaaaaa atactagatg
     4021 taagataaaa gtgtaaaaac gatttgtttt aggaggggaa acgatgtgta ccgcaatcac
     4081 ttatgcaacg aaagaccatt attttggaag gaattttgat tatgagatgt cttataatga
     4141 agtggtcact attactccaa gaaattatcg tttcgatttt agaaaggtaa aaaatttaga
     4201 taaacattat gcaatgatag gaatagcagc tggggtatcc aactatccac tctattatga
     4261 ggctacaaat gaaaaagggt tgagtatggc aggactgaat tttcctggca atgctgatta
     4321 taaagaatta caagaaggaa aagataacgt tgcaccattt gagtttattc catggatact
     4381 agggcaatgc tcaaccatag acgaagctaa agaattattg gctactatca atctagtcaa
     4441 tatcgatttt agtgaaaaac tacccttgtc acctttacac tggttattag ctgataaaga
     4501 aaaatcgata gtaattgaaa gtatgaaaga tggacttcat ttatatgata atcctgtcgg
     4561 tgtgttgaca aataatccgc catttgatta tcaattattt aatctgaaca attatcgctc
     4621 gctatcaaat gg
//
LOCUS       DACEEZ010000137         4607 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20313_2017
            SAMN12639966-rid9879223.denovo.142, whole genome shotgun sequence.
ACCESSION   DACEEZ010000137 DACEEZ010000000
VERSION     DACEEZ010000137.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639966
            Sequence Read Archive: SRR10023158
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4607)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4607)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741683.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741683.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:44:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,016
            CDSs (total)                      :: 2,962
            Genes (coding)                    :: 2,889
            CDSs (with protein)               :: 2,889
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 36 of 73
            Pseudo Genes (incomplete)         :: 29 of 73
            Pseudo Genes (internal stop)      :: 18 of 73
            Pseudo Genes (multiple problems)  :: 8 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4607
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639966-rid9879223.denovo.142"
                     /isolate="E20313_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(94..678)
                     /locus_tag="IWO89_002725"
     CDS             complement(94..678)
                     /locus_tag="IWO89_002725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007207927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HAQ0457416.1"
                     /translation="MKIGYARVSTGLQNLDLQEDSLNKFGCEKIFTDHMSGAKSNRPG
                     LEMAIDFVRSGDTLVVWRLDRLGRNMEDLISIVNRLNERGVSFHSIQENITMDKSSST
                     GQLMFHLFAAFAEFERNLILERSAAGRAAARARGRFGGRPEKLSEQDLELLKTLVDSG
                     TPIKTIAERWNVSRTTIYRYLAKMEEKNGKTNSH"
     gene            complement(889..1092)
                     /locus_tag="IWO89_002726"
     CDS             complement(889..1092)
                     /locus_tag="IWO89_002726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0457417.1"
                     /translation="MPEFVNTPNFYQDSDILSKINLPKEESALQVQRTLQETQKIQKS
                     ISNLEDSEEKRKKRDQKTYQKEK"
     gene            complement(1102..1524)
                     /locus_tag="IWO89_002727"
     CDS             complement(1102..1524)
                     /locus_tag="IWO89_002727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0457418.1"
                     /translation="MKNKFILSLGILTIGISVTGTTIPTFADSTNEPTTVVDNNQELT
                     PLDRDGIAIGNPTNDLTVTENYLIVSPLLALAASKTIRVTGTMIFDKVGWPSRPPQSR
                     NWKEKRNGYWYYGKLYLQSYKSAGNNWIATYSGTLTRK"
     gene            1762..2376
                     /locus_tag="IWO89_002728"
     CDS             1762..2376
                     /locus_tag="IWO89_002728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001261742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HAQ0457419.1"
                     /translation="MRVAYIRVSSIDQNEQRQIEEMKKFGAERIFIEKQSGATITHRP
                     VFQEALDYVRDQDIFIVEAIDRLGRNYDEIIASVNYLKKKNVKLIITSLPIMAEAIGN
                     PLLDKFIKDLIIQILAMIAEQERTESKRRQAQGIKIAKANGVYKGRPKLYSADAKDPQ
                     RRLVYKSIVEDLKNGVAIAKIAKDYNVTRQTVYRIKKDSMVNDK"
     gene            complement(2541..3365)
                     /locus_tag="IWO89_002729"
     CDS             complement(2541..3365)
                     /locus_tag="IWO89_002729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001809248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0457420.1"
                     /translation="MIERLKDFMISVWDVAMLADESNYTSSIYRLEDSNWIEISLELK
                     TLPNSVNYIIRDITSEAQQLVNYMHGHESIPFSLILLKRSKNISNTRDRFISIITACE
                     IGVKEFYQQEKPDLTLIFENLQSPPVDKLLGSMFKGYFNIEFPKEIRKEIKNFIEKRN
                     KLVHSPQGSKPTIEECFECYMAVLKTFNFFYKINDSFMYRDFYEESIELTPTEPNKSQ
                     ITLSKELERKVESQELAIGISITNVSTYKELYEAKSQDNKDFVENIDKEPTNTVGD"
     gene            complement(4003..4260)
                     /locus_tag="IWO89_002730"
     CDS             complement(4003..4260)
                     /locus_tag="IWO89_002730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Txe/YoeB family addiction module toxin"
                     /protein_id="HAQ0457421.1"
                     /translation="MIKAWSDDAWDDYLYWHEQGNKSNIKKINKLIKDIDRSPFAGLG
                     KPEPLKHDLSGKWSRRITDEHRLIYRVENETIFIYSAKDHY"
     gene            complement(4253..4522)
                     /locus_tag="IWO89_002731"
     CDS             complement(4253..4522)
                     /locus_tag="IWO89_002731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HAQ0457422.1"
                     /translation="MEAVAYSNFRQNLRSYMKQVNEDAETLIVTSKDVEDTVVVLSKR
                     DYDSMQETLRTLSNNYVMEKIRRGDEQFSKGAFKTHDLIEVESDD"
BASE COUNT         1514 a          811 c          623 g         1659 t
ORIGIN      
        1 aataagttat cagtagacac ttacaaaaaa ttctcatact attgtcactt ttctagataa
       61 atggcaaaaa ttttcagcat taaatgacaa aaactaatga gaatttgtct ttccattttt
      121 ctcctccatt tttgccaaat aacgataaat ggtggttctt gagacgttcc accgttccgc
      181 aatagtctta ataggtgtac cgctatccac taaagtcttc agtaattcca aatcttgttc
      241 agacaatttt tcaggacgac caccaaaccg tcctcttgca cgagcagctg ctcgtcctgc
      301 tgcggatcgt tccaggatca aattacgctc aaattctgca aatgccgcaa acaaatgaaa
      361 catgagctgt cctgtagaac ttgatttgtc catcgtaata ttttcttgaa tactatgaaa
      421 actcacacca cgctcattca gtcgattgac gattgaaatc aaatcttcca tatttctacc
      481 taaacgatct aagcgccaaa cgacaagtgt atcgccagag cgaacaaaat caatggccat
      541 ttctaatcct ggtcgattac tttttgcccc actcatatga tcagtaaaaa ttttttcaca
      601 accaaattta tttaggctgt cttcttgtaa atccaaattt tgtaaaccag tagatacccg
      661 ggcgtatcct attttcaaaa aaatcactcc ttcattaatt ttattgtaac ataactttat
      721 ccatatagtt taactgaaca tagatattgt tatgggttta tgaacatgaa atgaattgtt
      781 tttttgaaaa ataaagcaaa aaaagaaagt gtccagaaat gggtgatttt atgaacactt
      841 tcaattagaa aaaaagaatg tcgactagac actctttttt taaaatcatt atttttcttt
      901 ttgatatgtt ttttggtctc ttttttttct tttttcttca gaatcttcta gattagaaat
      961 tgatttttga attttttgtg tctcctgtaa agttcgctga acttgtaaag ctgattcttc
     1021 tttcggtaaa ttaattttac ttagaatatc gctatcttga taaaaatttg gtgtgttcac
     1081 aaattctggc ataaaaaatc cctactttct agttaaggtt cctgaataag tcgcaatcca
     1141 attgtttcca gctgatttat aactttgtaa atacaatttt ccatagtacc aataaccatt
     1201 tcttttttct ttccaattcc gtgattgagg aggtctagaa ggccaaccaa ctttatcaaa
     1261 aatcatcgtt cctgtcactc gtatcgtttt agaagctgct agagcaagta atggagaaac
     1321 tattaaataa ttttcagtaa cagttaagtc gttagttggg ttaccaatgg ctattccgtc
     1381 cctatctaaa ggagttaatt cttggttatt atctactaca gtagttggtt catttgtact
     1441 atcagcaaat gtaggaattg tagttccagt tacactaatt ccaatagtta aaatacctaa
     1501 agataaaata aacttatttt tcattgtcta tcacctcgaa atcaaaacta agaaaacgtt
     1561 ttcttaataa atcttaacag ataaatcaag ttatatacta gaaaattcat aaaatatatt
     1621 tttaatgtca taatcgttta aattttcttg attgacattt atttacaaaa aatattgcca
     1681 tttaaaaaga aaagtgacat atggcttcct cgtgtatgat tagggtgtat tttaaatagt
     1741 tgcagaaagt aggtaaaagt tttgagagtt gcatacatac gtgtctcatc gatcgatcaa
     1801 aatgaacaac gccagattga agaaatgaaa aaattcggtg cagagagaat tttcatcgag
     1861 aaacaatctg gcgcaacgat tactcatcga cctgttttcc aagaagcatt agattacgtc
     1921 cgagatcaag atatttttat tgtggaagct attgatcgac ttggaagaaa ttatgacgag
     1981 atcatcgctt cagtcaatta cttgaagaag aaaaatgtga agctgatcat tactagttta
     2041 ccgatcatgg ctgaagcaat tggcaatcct ctcctggata aattcattaa ggatctgatc
     2101 atccaaattt tggccatgat cgctgaacaa gaacggactg aatcgaaacg tcgccaggca
     2161 caaggaatta agatcgcaaa agcaaacggc gtctacaaag ggcggcccaa attatacagt
     2221 gcagacgcaa aagatcccca acgtcgcctt gtttacaaaa gcatcgttga agatcttaaa
     2281 aatggtgttg cgattgctaa gattgctaaa gactacaatg ttacgagaca aaccgtgtat
     2341 agaataaaaa aagacagcat ggtcaatgac aaatagttga tcatgctgct tttattatct
     2401 tctcatcttt aatttcctac atataattac tatctataaa attaagtctt actcagaaca
     2461 gataaagcat aaaattccaa caaaccattt tttacttaac taaataaaga gtctgttaat
     2521 ataatcttca cttatttacg ttaatcccct actgtatttg ttggttcctt atctatattt
     2581 tctacaaaat ccttattatc ttgtgattta gcttcgtata attctttgta tgtcgacaca
     2641 ttggttatac taattcctat agctagttct tgagactcta cttttctctc tagctccttt
     2701 gacaaagtga tttgagattt atttggttcc gttggagtca attctataga ttcttcataa
     2761 aaatctctat acataaagct atcattaatt ttataaaaaa aattaaatgt ttttagcact
     2821 gccatataac attcaaaaca ttcttctata gttggtttac ttccttgagg agaatgaacc
     2881 aatttattac gtttttctat gaaattttta atttcctttc ttatttcttt cggaaactca
     2941 atattaaagt accctttaaa catgctccct aataatttgt ctacaggggg agattgtaaa
     3001 ttctcaaata ttagagttaa atcaggtttc tcttgttgat aaaattcctt tacccctatt
     3061 tcacaggctg taattatcga aataaaccta tctctagtat ttgatatatt tttacttctt
     3121 ttaaggagta taagagaaaa ggggatactt tcgtgtccat gcatgtaatt aactaattgt
     3181 tgagcttcag aagttatatc tctaattata tagttaacgg aattaggaag tgtctttaac
     3241 tctaatgaaa tttctatcca attactatct tctaatctgt atatgctcga agtataattt
     3301 gattcgtcag caagcatcgc tacatcccaa acactgatca taaaatcttt taaacgttca
     3361 atcatttcag caatattgct aaaatcagct aatttttctt tagaataaaa aaaacattta
     3421 tatcagaatc aataaattct atttttttta ttactatatc gtcatcatta tcttgaccat
     3481 ttatgtttat tgttccctga gtggggagta cttctttcaa attcaagtta aatttatatt
     3541 ccattacttc tcctccaata tataaaaatt acttaactgt atccaaattt aatatgtaaa
     3601 taaatcatta atttttagct aaattctatg agaagtttat ttttgttgtt tttgctaatt
     3661 aactaaaact agtacaaaaa atttaaaata gattattaaa cctattgctt attctattca
     3721 gtcatatata taagcgtcat cctttagtaa ctaacatccc acactacaat agtattataa
     3781 catatctatt caacttcaaa ttgttgaaat ttaagccatt tatatttact tttcctcaac
     3841 tttagaaatg attttactaa ccttatatcc gtaatacgcg taacaaacac attcattaat
     3901 aacaatcaac actaagctat attagctttt taaatgacta gggtaaataa aataaggatc
     3961 aaaaactttt aagtttctga ccctttcctt acttccgatt ggttaatagt gatcttttgc
     4021 agaataaata aatatcgttt cattttcaac tctatatatc agtctatgtt catctgtaat
     4081 tcttctggac cattttccag ataaatcatg ctttaatggc tcaggttttc ctaatccagc
     4141 aaagggggaa cgatcgatat cttttattaa cttgttaatc ttttttatat tgcttttgtt
     4201 tccttgctca tgccaataaa gataatcatc ccaagcatca tcagaccaag ccttaatcat
     4261 cagattcaac ctcgattaag tcatgtgttt taaatgcacc tttggagaat tgttcatctc
     4321 ctcgacgaat tttttccatg acgtaattat tagaaagtgt tctcaacgtt tcttgcatag
     4381 aatcataatc tctttttgat aatacaacaa ctgtatcttc tacatcttta cttgttacaa
     4441 taagtgtttc agcatcctca ttaacttgtt tcatataact acgtaaattt tggcggaaat
     4501 ttgaataagc tactgcttcc attcctttca ccccttattt atttctataa aacaattgta
     4561 cattatattg tacaattaag caaatgatta atattctaac ttcttat
//
LOCUS       DACEEZ010000138         4472 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20313_2017
            SAMN12639966-rid9879223.denovo.144, whole genome shotgun sequence.
ACCESSION   DACEEZ010000138 DACEEZ010000000
VERSION     DACEEZ010000138.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639966
            Sequence Read Archive: SRR10023158
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4472)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4472)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741683.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741683.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:44:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,016
            CDSs (total)                      :: 2,962
            Genes (coding)                    :: 2,889
            CDSs (with protein)               :: 2,889
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 36 of 73
            Pseudo Genes (incomplete)         :: 29 of 73
            Pseudo Genes (internal stop)      :: 18 of 73
            Pseudo Genes (multiple problems)  :: 8 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4472
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639966-rid9879223.denovo.144"
                     /isolate="E20313_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            318..1049
                     /locus_tag="IWO89_002732"
     CDS             318..1049
                     /locus_tag="IWO89_002732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WxL domain-containing protein"
                     /protein_id="HAQ0457423.1"
                     /translation="MMKKEIVLTAAMMLFSMVASTTFVSATEVYPKEYNTEGTITFEA
                     GDEGVTPPVDPENPDPNKPVDPSDPPSPGTGGALSIDYGSKFKFGTQKISTADKTYYA
                     AADVMNDGSRKPTYVQVTDRRSTLSGWKLSVSQPEQFKTASGDELVGAQLKFTKGQAV
                     SLVDPTYTPQTVNSELTLTPGGNNTLAINAKSGTGVGTWVYRFGANENENQDAVQLSV
                     PGKSVKLAQQYSTKLVWTLEDTPNN"
     gene            1194..3221
                     /locus_tag="IWO89_002733"
     CDS             1194..3221
                     /locus_tag="IWO89_002733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lectin"
                     /protein_id="HAQ0457424.1"
                     /translation="MKKRRIVLILSSLLVTNIFPIIGYSLELNQLEHGNRRDEESKLT
                     STFDSLIESNQTQESSEDLSISSSGETTDNVEAEGSDKQGLEDAPISAVELPDDPNII
                     SIDKIFQTPIGTSTSILENGKLLQLNPAERSQRGAIWSNKKISLLSDFTFKSYLYLGN
                     QRDRAGDGMTFTLTGDPRMASNPSQVIGSPGMGIGSYSTRSGQPYIANALSIEFDTYK
                     NSGSSDRMDREITADNRGHGHVAFVTPKPNNNNYSGEHTGVTVAPTFLSNGTWRLLII
                     KWDAEQRSLTYDLEGVGHSSRIIPDLIRQFGSTEVNWGFTSSTGTFFQENAVAVTRLP
                     SNVTSTADVKVNDSDYGAIAEAGGNDKITIRNTLKVEGDFLEYKKMQTSIDLPQELTI
                     PEHILINGNQIDAKDILITGNRVTLNLTDYFIAGDTLIIELETTLQEVIPEKVLTMHF
                     EYLENGNLIEQSNEINLKIAKLKEIDIHVYYKDKDSQIDIAEMKTITGKIGEKYKETP
                     IDIEGYVFDSDSANTEGIFSENTEDIYFYYRKGKLYLSTAPQYVNFGKHKISSRPLSE
                     FGQFSGGLKIIDERAFGSWKLQLKQEKVLTNGDFELPEALSFVTSENTTVIGTSAVTI
                     FESDQKGESDLSSLLDSTAHRGIRIDIPVENQRIGTFEGKLSWILADVPGN"
     gene            3329..4426
                     /locus_tag="IWO89_002734"
     CDS             3329..4426
                     /locus_tag="IWO89_002734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF916 and DUF3324 domain-containing protein"
                     /protein_id="HAQ0457425.1"
                     /translation="MIKGAIFLRPKQQLFITLLVILGALFNNISVSASAFNFSVTPIT
                     SENQIDKRKTYFDLQLDPDQEVEVKAELRNDTEKEVKIDISVNSATTNSNVMVEYGKN
                     EIEKDMSLIFDLVDYVSYPQIVTLKPKSVQTVAFHVRMPNERFDGVLAGGITFQEIQT
                     EKDQTETKDQSLSIENEYTYIIALLMQQTLNEVAPNLTLHEVKPDQINARNVILANVQ
                     NDQKTYINQVVIETKITKKGHSEVLYQEEKEGLQIAPNTNFSFPTALNGQPLTPGEYH
                     LTMTVLGNENENGKFSRKKGNTTINYTNQWVFEKDFTIDGKVAKELNTTDVTIKKDNN
                     WIYILVASLFLLFILLCILLFVLRKKEEKEK"
BASE COUNT         1569 a          717 c          925 g         1261 t
ORIGIN      
        1 atataatata cattttttat ttaattataa ttaatattga ttgaagtcta ggcagtcaag
       61 tcacattttg atagatagga gggggactta cgaattattt taaaggctat gacagctgta
      121 tttctagatt gtgtttgcat caagttgcaa taaaaactag aaatcaaatc gggctgataa
      181 tcatttataa ctaatcgata cataggacca aatataggtc tatgttcttt tatttcataa
      241 gtatcgtaaa acagaatttt gctatggatt aatccaatcg actgatatgt ttagtaaata
      301 aagaaagagg aatgtcaatg atgaaaaaag aaatcgtatt aacagcagca atgatgttat
      361 tcagcatggt agcaagtacg acttttgttt ctgcaactga agtataccca aaggaatata
      421 acacagaagg aacgattaca tttgaagcag gagatgaggg tgtaactcct cctgttgatc
      481 cagagaatcc tgatcccaat aaacctgtcg atccatcaga tcctccaagc cctggaacag
      541 gaggggcatt atcgattgat tatggatcaa aattcaaatt tgggactcaa aaaatttcta
      601 ctgcggataa aacgtattat gcagctgctg atgtgatgaa cgatgggtct agaaaaccga
      661 catatgtcca agtgacagat aggagaagta ccttatctgg gtggaaatta agtgtctctc
      721 aaccagaaca gtttaaaact gccagtgggg atgaactagt tggagcacag ctgaaattta
      781 cgaaagggca ggctgtttct ttggttgatc caacatatac gccgcaaacg gtgaattctg
      841 aattaacgtt gacaccggga ggaaacaata cgttagcaat taatgctaaa tcaggaacag
      901 gtgtggggac ttgggtctat cgatttggtg caaatgaaaa tgaaaatcaa gatgcggtac
      961 agctttcggt acccggtaaa tctgtcaagt tagcgcaaca atattccact aaattagttt
     1021 ggacattaga agatacacct aacaactagt ttactgaaaa gaactgttaa aaataaatac
     1081 gtagtcatat tgtcttgcga ggtaatcgag caaaaattgt caaaggctag gctaaatttg
     1141 tttggatacg aatagttcat ctaggaattt aacgatcagg aggaaaagat atattgaaaa
     1201 agaggaggat cgtgctcatt ttaagcagtc tcttagtgac taatatcttt ccgatcatag
     1261 ggtactcttt ggaattgaat cagttggagc atggaaatag aagagacgaa gaaagcaaac
     1321 tcacttcgac attcgacagt ttaatagaat ccaaccagac acaagaaagc agtgaggatt
     1381 taagtatttc gagtagtgga gagaccactg acaacgtaga agctgagggt tcggacaaac
     1441 aaggactaga agatgcacca atttctgcgg ttgagctgcc tgacgatccc aatattattt
     1501 ctatcgataa aatttttcaa acacctatcg gtactagcac gtccatattg gaaaatggga
     1561 agttactgca gttgaaccca gcagaaaggt ctcaaagagg agctatttgg tcaaataaga
     1621 agattagttt actctcggat tttacattta aatcttattt atacttagga aatcaacgag
     1681 atagagcggg agatggcatg acctttacat tgactggcga tcctagaatg gctagtaatc
     1741 ccagtcaagt tattggatcg ccaggtatgg gaattggctc atattctact cgttctggcc
     1801 aaccttacat tgcgaatgcg ctctcgattg agtttgatac gtataaaaat agtggttctt
     1861 ctgatcgaat ggatcgagag ataactgctg ataatagagg gcatggacat gtcgcttttg
     1921 tcacgcctaa accaaataat aataattatt ctggagagca tactggcgtg acggtagctc
     1981 ctacatttct ttctaatggc acatggcgct tgttaataat taaatgggac gcggagcaaa
     2041 gaagtcttac ttatgatttg gaaggtgtcg gtcactcttc tcggattata cctgatctca
     2101 ttaggcagtt tggttctaca gaagtaaatt gggggtttac ttcttcaaca ggtacttttt
     2161 ttcaagaaaa tgctgtcgct gtcacgcgac ttccatcgaa tgtcacatca acagctgatg
     2221 taaaagtcaa cgatagtgat tatggggcca tcgctgaagc tggaggaaac gataagataa
     2281 ctatcagaaa tacattgaag gtcgaggggg attttcttga atataagaaa atgcagacta
     2341 gtatcgacct cccacaagaa ttaactattc ccgagcatat cttgatcaat ggaaatcaaa
     2401 ttgatgccaa agatatcttg ataaccggaa atcgagtcac gctaaatctg acagattatt
     2461 ttattgctgg agatacgtta ataatcgaat tggagacaac tttgcaggaa gtaattcctg
     2521 aaaaagtact cactatgcat tttgaatacc ttgaaaacgg gaatttgatt gagcaatcga
     2581 atgaaataaa cctaaagatc gcaaagctga aggagataga tatccatgtt tattataaag
     2641 ataaagacag tcagattgat attgctgaaa tgaaaacaat cactgggaaa ataggagaga
     2701 agtataaaga aactcctatt gatattgaag ggtatgtttt tgactcggat tcagcgaata
     2761 cagaaggcat cttctccgaa aatacagagg atatctattt ttattacaga aaaggcaaac
     2821 tttatctctc aactgctcct caatatgtca attttggaaa acataaaata tccagtagac
     2881 cattaagtga attcggacag ttttcaggtg ggttgaaaat tatagatgaa cgggcttttg
     2941 gaagttggaa attacaattg aaacaagaga aagttcttac aaatggagat tttgagcttc
     3001 cagaagctct ttcctttgtt acctctgaaa atacaacggt tatagggaca tccgctgtta
     3061 cgattttcga aagtgatcaa aagggtgaaa gcgatttgtc ctctctttta gattctacag
     3121 ctcatcgtgg aatcagaata gatattcctg tagaaaacca aagaatcgga acctttgagg
     3181 ggaaactatc ttggatactt gcagatgtcc caggaaatta acaggaagag aagaatataa
     3241 caaaattatt gagtttcaag aagggcacag gtaccatagg ctcttgtgtc ccacagagac
     3301 aagattttgt tatagtcttc tcttacatat gattaaagga gcgattttct tgagaccaaa
     3361 acaacaatta tttataacac tcctagttat tttaggggca ctttttaaca atatatcagt
     3421 gtcagctagt gcatttaatt tttcagtgac gccgataact tcagagaatc aaatcgacaa
     3481 gagaaaaact tacttcgatc ttcaacttga tccggatcag gaagtagaag taaaagcgga
     3541 attgagaaat gatacagaaa aagaagtgaa aatcgatatt tctgtgaata gcgcaaccac
     3601 caattcaaat gtgatggttg aatacggaaa aaacgagatt gaaaaagaca tgagcctgat
     3661 ctttgatctg gtagattatg tgagctatcc acagatagtg acgctaaaac ctaaaagtgt
     3721 ccaaaccgtt gcttttcatg tcaggatgcc gaatgagcga tttgatggtg tattggctgg
     3781 tgggatcaca ttccaagaaa tccagacgga aaaggaccaa acagaaacta aggatcaaag
     3841 cttatcgatt gagaatgaat acacctatat tattgctttg ttgatgcaac agacactgaa
     3901 cgaagttgct ccgaacctta ctcttcatga agttaaacca gatcaaatca atgcaagaaa
     3961 tgtcatctta gcaaacgttc aaaatgatca aaaaacctat atcaatcaag tagttattga
     4021 aacaaaaatt acaaaaaaag gacattctga agttttatat caagaggaaa aagaaggatt
     4081 gcagattgcg ccgaatacaa atttttcttt tccaactgct ttaaatggtc aaccgctaac
     4141 cccaggtgaa tatcacctta caatgactgt tttaggaaat gaaaatgaaa atggaaaatt
     4201 ttcgagaaaa aaaggaaata ctacgatcaa ctataccaat caatgggtat ttgaaaaaga
     4261 ttttacaata gacggaaaag tagccaagga attgaacacg acggatgtta cgattaaaaa
     4321 agacaataat tggatttata tcttagtagc ttcacttttc ttattgttta ttttattatg
     4381 catacttttg tttgttttga gaaaaaaaga agagaaagaa aagtaaggtt aagataacaa
     4441 atagtaaatt tttattattg taggatggtt gg
//
LOCUS       DACEEZ010000139         4433 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20313_2017
            SAMN12639966-rid9879223.denovo.145, whole genome shotgun sequence.
ACCESSION   DACEEZ010000139 DACEEZ010000000
VERSION     DACEEZ010000139.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639966
            Sequence Read Archive: SRR10023158
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4433)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4433)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741683.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741683.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:44:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,016
            CDSs (total)                      :: 2,962
            Genes (coding)                    :: 2,889
            CDSs (with protein)               :: 2,889
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 36 of 73
            Pseudo Genes (incomplete)         :: 29 of 73
            Pseudo Genes (internal stop)      :: 18 of 73
            Pseudo Genes (multiple problems)  :: 8 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4433
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639966-rid9879223.denovo.145"
                     /isolate="E20313_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(543..2048)
                     /locus_tag="IWO89_002735"
     CDS             complement(543..2048)
                     /locus_tag="IWO89_002735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HAQ0457426.1"
                     /translation="MIKPKKSEKRELKGPSLTIKWAFASSFFIFVVFTIFAVITYKSS
                     INLIVAKERNNVERTISEVASRLANSDEELTLVNTYRNLTKATNDGDEVVDHTSLVEG
                     NLMKIDSFIAELGQPSMDLYVYDLDENLIFKTHENSRNLIQTTRKVPTIVTLEGKTGF
                     LSIQPIYSKGTREKIGYAQSFYELSSFYDIRNKLLLTLIVLEVVSLILSSILGFFLSS
                     YFLKPLKVLRDTMDTIRKDPQSDIHMPEIDTNDELADLAEIFNEMLDRMRLYIEQQEQ
                     FVEDVSHELRTPVAIIEGHLSLLNRWGKDDPEILEESLSASMQEISRMKSLVQEMLDL
                     SRAEQVDVHYANETTKGKEVTYQVYNNFKILYPDYVITLDDDLTKEVTLQIYRNHFEQ
                     LIIIILDNAVKYSTTRKEVHISISSTLNEFEIAIQDFGEGIPKEDLNKIFNRFYRVDK
                     ARARTKGGNGLGLSIAKQLVENYKGRILAESVVGQGTIFRIFIPIAKAEDK"
     gene            complement(2045..2731)
                     /locus_tag="IWO89_002736"
     CDS             complement(2045..2731)
                     /locus_tag="IWO89_002736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010763438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAQ0457427.1"
                     /translation="MSNILIIEDEKNLARFVELELKHEGYNAEVHYNGRTGLDAALNN
                     EWDAILLDLMLPELNGLEVCRRVRQVKNTPIIMMTARDSVIDRVSGLDHGADDYIVKP
                     FAIEELLARLRALLRRIDIEGDKNVAKQTTITYRDLTIEKENRVVRRGNEVIELTKRE
                     YELLLTLMENVNVVLARDVLLNKVWGYETEVETNVVDVYIRYLRNKIDVPGEESYIQT
                     VRGTGYVMRS"
     gene            complement(2924..4345)
                     /gene="gndA"
                     /locus_tag="IWO89_002737"
     CDS             complement(2924..4345)
                     /gene="gndA"
                     /locus_tag="IWO89_002737"
                     /EC_number="1.1.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent phosphogluconate dehydrogenase"
                     /protein_id="HAQ0457428.1"
                     /translation="MSKQQIGVVGMAVMGKNLALNIESRGYSVALFNRTGAKTTAVVE
                     EHPDKNFKATYTIEEFVESIEKPRRILLMVKAGPATDATIQELLPHLDKGDILIDGGN
                     TFFKDTMRRNEELANSGINFIGTGVSGGEEGALKGPSIMPGGQKEAYALVAPILEQIS
                     AKAEDGAPCVTYIGPNGAGHYVKMVHNGIEYGDMQLIAESYDLMKNILNLSVEEMADI
                     FKEWNQGELDSYLIEITADILTRKDDEGTGKPVVDVILDAAGNKGTGKWTSQSALDLG
                     VPLPLITESVFARYISAYKEERVQASKILSRTNDFEFTGDKKELVEKIREALYFSKIM
                     SYAQGFAQLRVASKEFDWDLPFGEIAKIWRAGCIIRARFLQKITDAYDKNPEIENLLL
                     DDYFVEITKKYQQSVRDVVALAVQAGVPVPTFSSAIAYFDSYRAERLPANIIQAQRDY
                     FGAHTYERVDKEGIFHYSWYHEE"
BASE COUNT         1239 a          919 c          729 g         1546 t
ORIGIN      
        1 tctttttgtt ttaacaactc tttaatctta tcagattcag aagagaatgt caacaacttc
       61 tttgtttatt ttttctaaac gtctttgcca ctcattagca gcgacttgat tatcatacca
      121 tctttattgt tatttgtcaa taactttttc gaagtttttt tcgataaaat caagctttat
      181 tagctatctc atccaaacga cttcgttatt ataacatgac atcaaaagtg cgtcaacact
      241 attttttata atttttagtg acaccttaaa ggaatcattt agaacaaata ataatatagc
      301 aatctcttta taaaattgca atagaaaatt ccctattttt atttattatt tttaaacacg
      361 aatattacag cattttattg ttttttcgtt catctaaaag atacttacat ttaattaacg
      421 gatgttattc tatttaattg ttcgctagtt tttttataat gacatccctg aatttctcac
      481 ttgcttgtat tttgtacctt attctttgtt ggatatataa aaaaacaagt aaagcacatg
      541 ctttacttgt cttctgcctt tgcgatagga ataaagattc taaaaattgt cccttgccct
      601 actacacttt ctgctaagat acgtccttta tagttttcaa ccagttgttt agcgatagac
      661 aaccccagac catttccacc cttcgttcta gctcttgctt tatccactcg ataaaaacga
      721 ttgaagatct tattaaggtc ttctttgggt attccttcgc caaagtcctg aatcgcgatc
      781 tcaaattcat tcagagtgga agaaatagag atatggactt cttttcgtgt agttgaatac
      841 tttactgcgt tatccaatat gataatgatt aattgttcaa agtgattacg ataaatttgc
      901 agggtcactt cttttgtcaa atcatcatct aaagtgatca catagtctgg atataatatc
      961 ttgaaattat tataaacttg ataggtgact tcttttcctt tagttgtttc attcgcataa
     1021 tgaacatcta cttgttctgc tcttgataag tctagcatct cttgaactaa gcttttcata
     1081 cggctgattt cttgcatgct ggcgcttaat gattcttcta atatctcagg atcatctttc
     1141 ccccaacgat ttagcagact aagatgccct tcaataattg caacaggtgt ccttaactca
     1201 tgagatacgt cttccacgaa ttgctcttgt tgttcaatat acaaacgcat acggtcaagc
     1261 atctcattga atatttcagc taaatctgcc aattcatcat ttgtgtcgat ttcaggcatg
     1321 tggatatctg attgcggatc tttacgtata gtatccattg tatctctgag taccttgagt
     1381 ggttttaaga aataagaaga taaaaagaaa cctaaaatac tacttaagat caaagaaaca
     1441 acttctaaaa caataagagt caataatagc ttatttcgaa tatcatagaa agaagataat
     1501 tcatagaagc tttgcgcata gccgatcttt tctcttgtac ctttagaata tataggctgg
     1561 atagataaaa agccagtttt tccttctagt gtaacgatcg ttggaacttt acgtgtagtc
     1621 tgaatcaggt ttcgactgtt ttcatgggtt ttaaaaatca agttctcgtc caaatcataa
     1681 acataaagat ccattgatgg ctgccctagc tcagctataa aggaatcgat cttcattaag
     1741 tttccttcga ccagtgaagt atgatctacg acctcatctc cgtcatttgt cgctttcgtc
     1801 agattacgat acgtatttac taatgtcagt tcttcatccg agttagccaa acgagaagct
     1861 acttctgaaa tcgtacgctc tacattgttt ctttcttttg ccacaatcag attgatagat
     1921 gatttatagg taatgacagc gaaaattgta aacacaacga atatgaagaa agaactcgca
     1981 aaggcccact tgattgtcaa ggagggccct ttcagttctc ttttttccga ttttttcggc
     2041 tttatcacga gcgcatcaca taacctgttc cgcgtacagt ttggatatag ctttcctccc
     2101 cgggaacgtc aattttgttg cgaagataac gaatatatac gtccactacg tttgtttcaa
     2161 cttctgtttc gtatccccac actttattta gtaaaacgtc acgtgctaat accacgttca
     2221 cgttttccat taaagtaagc agcaactcgt attctctttt tgttagttca attacttcat
     2281 ttccgcgacg tacgacacgg ttttcttttt cgatagtcaa atcgcgataa gtaatagtag
     2341 tttgtttagc cacattttta tcgccttcaa tatcgattcg acgcaacaat gcacgaagac
     2401 gtgctaataa ttcttcgata gcaaatggtt tgacgatgta gtcatctgct ccgtggtcaa
     2461 gtccagatac acgatcgatc acagaatctc ttgcagtcat cataatgatt ggtgtatttt
     2521 tgacttggcg tacacgtctg cagacttcta aaccgttcaa ttctgggagc atcagatcca
     2581 acaatatggc atcccactca ttatttaatg ccgcatctaa accagttcgg ccgttgtagt
     2641 ggacttctgc gttataccct tcatgcttca actctagctc gacaaatcgt gccaagttct
     2701 tttcatcttc aatgattaga atattgctca tttattattt tatcctttct attctactgg
     2761 ctttttaaaa actctatcat tataccgata atatgcttaa aaatctatag tcattttatc
     2821 atggatagca attttaaaaa agagctgaag tctactcata acaatcgact tcagctcttt
     2881 cgtcctcttt ttattttctc atttcctaac agccgaaaga ctattattct tcatggtacc
     2941 atgagtaatg gaagattcct tctttgtcga cacgctcata ggtatgtgcg ccaaagtaat
     3001 cgcgttgggc ttggattata tttgcaggca gacgttctgc tctgtatgaa tcgaagtaag
     3061 cgatggctga tgagaaagtc ggtacaggta cgccagcttg gacagccaag gcaacgacgt
     3121 ctctgactga ttgctgatat tttttcgtga tttcaacgaa gtaatcatct agcaataaat
     3181 tttcgatctc aggatttttg tcataggcat ccgtaatttt ttgcaagaat cgagctcgga
     3241 tgatacatcc agcgcgccag atttttgcga tttcaccaaa tggcaagtcc caatcaaatt
     3301 cttttgaagc gacacgtaat tgagcaaatc cttgggcata actcatgatt ttactgaaat
     3361 acaacgcttc tcggattttt tcgaccaatt cttttttgtc tcccgtgaac tcaaaatcgt
     3421 tcgtacgaga caaaatcttg cttgcttgta cgcgttcttc tttataagca gaaatatagc
     3481 gggcaaatac agattcagtg atcaatggaa gtggcacacc taagtcaagc gcgctttggc
     3541 ttgtccattt acctgttcct ttattcccag ctgcatcaag gatcacatct actactggtt
     3601 tacctgttcc ttcatcgtct ttacgtgtca gaatgtcagc agtgatttct atcaaatagc
     3661 tgtctaattc gccttgattc cattctttaa agatatcagc catttcttct actgaaaggt
     3721 ttaaaatatt cttcattaag tcataagatt cagcaatcag ttgcatatct ccatactcaa
     3781 taccgttgtg taccattttc acataatgtc cggctccatt tgggccaata tatgtgacac
     3841 atggtgctcc gtcttctgct tttgcagaaa tttgctctaa gattggtgca actagcgcat
     3901 aggcttcttt ttgtccacca ggcatgattg aaggtccttt taaagctcct tcttcaccac
     3961 cggaaacgcc tgtaccgata aagttgatgc cagagttggc taactcttca ttacgacgca
     4021 tcgtatcttt aaagaatgta tttcctccgt caatcaaaat atcgccttta tcaaggtgag
     4081 gaagtaactc ttggattgtt gcatctgttg ccggtcctgc tttgaccatt agcaagatcc
     4141 gacgtggttt ctcgatagat tctacaaatt cttcgattgt atatgttgct ttgaaatttt
     4201 tatctgggtg ttcttctact acagcggttg ttttagcccc tgtacgattg aataaagcca
     4261 ctgaatagcc gcggctttct atgttcaagg ctagattttt acccataacg gccataccga
     4321 caacgccgat ttgttgtttt gacatatgaa ctcctcctac atacgggatt tctccctaat
     4381 ctcttacatt atagcttaac acggctacat tgcaaactaa agaacaacat att
//
LOCUS       DACEEZ010000140         4425 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20313_2017
            SAMN12639966-rid9879223.denovo.146, whole genome shotgun sequence.
ACCESSION   DACEEZ010000140 DACEEZ010000000
VERSION     DACEEZ010000140.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639966
            Sequence Read Archive: SRR10023158
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4425)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4425)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741683.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741683.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:44:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,016
            CDSs (total)                      :: 2,962
            Genes (coding)                    :: 2,889
            CDSs (with protein)               :: 2,889
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 36 of 73
            Pseudo Genes (incomplete)         :: 29 of 73
            Pseudo Genes (internal stop)      :: 18 of 73
            Pseudo Genes (multiple problems)  :: 8 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4425
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639966-rid9879223.denovo.146"
                     /isolate="E20313_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            690..1658
                     /locus_tag="IWO89_002738"
     CDS             690..1658
                     /locus_tag="IWO89_002738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HAQ0457429.1"
                     /translation="MDVAKTVNAIVEKNDGEISGIYLVACGGSLVDMYVSDYFLKAEA
                     SKCITGLYTANEFNHVTPKQLDDKSVVILCSHGGNTAETVKAGEVAQARGAVTVGLTH
                     NKTADLLKVSDHSFLYEWGDDAKIVNNPMAIILDLTVSLVNVTETFEHYGKFREGMKV
                     IDTVIENGIKQVQDRIKVFADKYKDETMFYILGSGPSYGHAYGFSICSLMEMQWLDST
                     SVHSGEFFHGPFEVTDKDTNFILLMSIGRTRALDERAKRFLDKYAERVEIVDAKELGL
                     DMISDEVSEFFNPILFYSILSEYRSALADVRNHDLDVRRYMGKVEY"
     gene            1669..2484
                     /locus_tag="IWO89_002739"
     CDS             1669..2484
                     /locus_tag="IWO89_002739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016172985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructoselysine 6-kinase"
                     /protein_id="HAQ0457430.1"
                     /translation="MKVLGLGDNVVDKYENLGIMYPGGNSLNFAVFAKKLGHESSFMG
                     VFGSDEEAAHVLSAIKEIGLDNSHSRFEKGENGCARVKIDEGDRIFLGSNSGGVTREY
                     PIQLTEEDREYLNQFELIHMGLYSHVNHLLEELQNVSGKISYDFSDDFTEEEVQRAID
                     KVDFGFFSIEDFSDEEVKQFLMKHFCERNDVLIATRGEKTAIGYDGAKFYEVNPVLEE
                     PVDTMAAGDSFLTAFLLYFLEGDDIVTAMKKGNEFAGKSCLVDGSFGFGIHYD"
     gene            2552..3262
                     /locus_tag="IWO89_002740"
     CDS             2552..3262
                     /locus_tag="IWO89_002740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016172984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAQ0457431.1"
                     /translation="MLDTNAQLPLYLQLKEILKNKINTGEYPENTKIPAESELIESYE
                     VSRITVRKAVEELVEEGYLLKKQGKGTFVNRHKVFRKIEYIMGFSESCKSNGFTPTSI
                     VLERKIIPATEGMAKKLDIAQGDDVLYIQRKRMADNIPIMLENNYFSYERYSFLMEEE
                     LTDSLYEVLKVQGTLAINPGETALEIVTADDQIAKIMEVAVGTPFFFMDTLINDQNNI
                     PIHVGNQYYLGEVYRFYL"
     gene            3298..>4425
                     /locus_tag="IWO89_002741"
     CDS             3298..>4425
                     /locus_tag="IWO89_002741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HAQ0457432.1"
                     /translation="MKQILRSKMIFDGEQPPFKGYIVINEGIIEKVENNWNYSDLLNE
                     NTELIAYDDQFVMPAIHDNHVFFSGYLAMNAGIDLSDTSSANEAVERIKAVMNHKKAD
                     EPIYAHGWDAALWKTDPSEELLNEINHFGPITAIDKNRSHCWMNAIAKQTYGFTEKET
                     SAESRVLLIKEMLANKKLLAEVYAEFEALLLSKGVISMKEIVFDDAEFLSNIPQRKIL
                     SNLYVQAVQEPLQHELLYEYKEKVFPEKVRFGGVKIMVDGVVADRSGDIYGVYQSGVA
                     SPEIDYEAIKAEVAAFNEVEIPCCLTTEGDKAGYKAASILVEFGKRLPEGVFNSISDL
                     EMVTQKTAEKMNEGQVVAEVYPQILGLNQTLEEAYMSTVIKN"
BASE COUNT         1490 a          661 c          921 g         1353 t
ORIGIN      
        1 atacaaataa ttcaccaaaa aggtgagtaa tgccattttt gaaaattgag cttgttttat
       61 gtctacatct gtatccgtat ataatccaaa ggattataaa ctcagaagag tctttatttt
      121 ttctgaatat aactactgta tcaaaaacaa tcgattatga gacaaaacaa aatgacctct
      181 tttatttaag aaaggaggtc attttttgta tcataaattg tattaaaatc aatgtatcac
      241 tttttaatca gttttaagac cataaattgc gtactaaaag aaattctgca attcaaaata
      301 aagcatttta agccattttc aaatttaagt gcacccatct acccgtcaaa tcactttatt
      361 ttccactcca gtagtttcct aattcaactg tgtgcaaaaa cgatttcttc taaacaaatc
      421 aagacaaact tataaacctt catcccagca ccataattat ttaaatcata ggcatttttc
      481 gaaattagta tatgtactca agtttcactg cagattattt atatttttta gagaaataaa
      541 tttttcctat tgacaaacac aaagaaagcg gatacaatgc aattatgtca catgacgtta
      601 tatgacgtta tgtgatatct ttacatttaa tcagttactt ttattttgga taaaattaac
      661 atttttaaaa ttttaaggag gaaatgttga tggatgtagc aaaaacggtt aacgcaattg
      721 tagaaaaaaa tgatggagag atttctggaa tttatttggt ggcttgtggc ggttcgctgg
      781 tagatatgta tgtctcagat tattttctta aagccgaagc atcgaagtgt attacaggct
      841 tatatacagc gaacgaattt aaccatgtaa ccccaaagca attagatgat aaatctgttg
      901 tcattctatg ttctcatgga ggaaatacag cagaaacggt aaaagcagga gaagtcgctc
      961 aagctagagg agctgtgact gttggtctga ctcataataa aactgcggat ttattaaagg
     1021 tttctgatca tagtttcctt tacgaatggg gagatgatgc aaaaatagtc aataatccta
     1081 tggccatcat tcttgattta actgtatctt tggtcaatgt taccgaaaca tttgaacatt
     1141 acggtaaatt cagagaaggc atgaaggtta tcgataccgt aattgaaaat ggcattaagc
     1201 aggtgcagga tcgtattaaa gtgtttgctg ataagtacaa agatgaaaca atgttttata
     1261 tcttaggtag tggaccatca tatggacatg cttatggttt ttcaatttgc tctttgatgg
     1321 aaatgcaatg gcttgattca acttctgttc attctggaga attcttccat ggaccttttg
     1381 aagtaacaga caaagatacg aattttattc tcttgatgag cattggacgc actcgtgctt
     1441 tagatgaacg ggcaaaacga ttccttgata aatatgcaga aagagtagaa atcgttgatg
     1501 caaaggaact aggattggat atgatatcag atgaagtttc tgaattcttc aatcccattt
     1561 tattctacag tattctttca gaatatcgtt cagctttagc agatgtacgc aatcatgatt
     1621 tagatgttcg tcgctatatg gggaaagtgg agtactagga ggattaaaat gaaggtatta
     1681 ggcttaggtg ataacgtcgt cgataaatat gaaaatctgg gaattatgta tcctggagga
     1741 aattcattga attttgcagt ttttgcaaaa aaactgggac atgaaagtag ctttatggga
     1801 gtttttggct cagatgagga ggcagctcat gttttatcag caataaaaga aataggactg
     1861 gacaacagtc actctcgctt tgaaaaaggc gagaatggct gtgccagagt gaagattgat
     1921 gagggcgacc ggattttcct tggcagcaat tcgggaggtg ttactcgtga atacccgatt
     1981 cagcttactg aagaagacag agaatattta aaccagtttg aattgattca tatgggatta
     2041 tatagccatg tcaatcatct attagaagag ttacaaaatg tttctggaaa gatttcttat
     2101 gatttttctg acgactttac ggaagaagag gttcagcggg caattgataa agttgatttt
     2161 ggcttctttt ctatagaaga cttttccgat gaagaagtaa agcagttttt gatgaagcat
     2221 ttttgtgagc gaaatgatgt attgattgca acccgaggag aaaaaacggc tattggttat
     2281 gatggagcaa agttttatga agtgaacccc gtgctagaag aaccagttga tacaatggca
     2341 gcgggagatt ctttcttaac agctttccta ctttattttc tcgaagggga cgatatagtc
     2401 accgcgatga aaaaggggaa tgaatttgct gggaagtctt gtctggttga cggttcgttc
     2461 ggctttggta ttcattacga ttgagcttgt tttaaattcc tgcatttgat aacctaaaga
     2521 aagaaatccc ggtaaagtgg aggttaaaaa aatgctggat acaaatgcac aattaccatt
     2581 gtacttacaa ttaaaagaaa tattaaaaaa taaaatcaat acaggggagt atcccgagaa
     2641 tacgaaaatt cccgctgaat cagagctgat agaaagttat gaggtcagcc gaatcacagt
     2701 gagaaaagca gtagaagaac tggtagaaga agggtaccta cttaaaaaac aagggaaagg
     2761 aacttttgtc aaccggcata aagtttttcg taaaattgaa tatattatgg gtttcagtga
     2821 gagttgtaaa tctaatggtt ttacaccgac tagcattgtt ttggaacgca agatcattcc
     2881 agcaactgaa gggatggcaa aaaaactcga tatcgctcaa ggtgatgatg ttttatatat
     2941 ccagagaaag cgaatggcgg ataacatccc cattatgtta gaaaacaatt atttttctta
     3001 tgaaagatat agtttcttaa tggaggaaga gctgactgat tctttgtatg aagtacttaa
     3061 agtccaaggt accttagcca tcaatcctgg ggaaactgca cttgaaattg tcacagctga
     3121 tgaccaaatt gccaaaataa tggaagtagc tgttgggacg ccctttttct ttatggatac
     3181 tttaatcaac gatcaaaaca acataccaat tcatgtaggt aatcaatatt acctgggaga
     3241 agtatatcgt ttttacttat gatttcctgt ttggatttca aggagtagat tttatttatg
     3301 aagcaaatat tgcggtcaaa aatgattttt gatggagagc agccaccttt caaaggttat
     3361 attgtaatca atgaagggat cattgaaaag gtggaaaaca actggaatta cagtgatcta
     3421 ttaaatgaaa acacggaatt aattgcttat gatgatcaat tcgttatgcc agcaattcat
     3481 gataatcatg tatttttcag tggttatttg gctatgaatg ctggcataga tttaagcgat
     3541 acatcttcag ctaacgaggc tgtagagcgt attaaagctg taatgaacca taaaaaagca
     3601 gatgaaccaa tttatgctca tggctgggat gctgccctgt ggaaaactga cccatcagaa
     3661 gagctattga atgaaataaa ccactttggt ccaatcacag cgatcgacaa aaatcgcagc
     3721 cattgttgga tgaatgctat tgctaagcag acttatggtt ttacagaaaa agaaaccagt
     3781 gctgaaagtc gagtattatt aattaaggaa atgctagcaa acaaaaagct tttagcagag
     3841 gtttacgctg aatttgaagc attactgctt tcaaaaggtg ttatatcaat gaaagaaatc
     3901 gtttttgatg acgcagaatt tctttctaat atacctcaga ggaaaatact tagtaaccta
     3961 tatgtgcagg cagtccaaga acctctacag catgaacttc tatatgagta taaggaaaaa
     4021 gttttccctg aaaaggtccg ctttggtgga gtgaagatca tggtagatgg tgttgttgcg
     4081 gatagaagtg gagatattta cggtgtttat cagtcaggag tagcttcacc cgagattgac
     4141 tatgaggcaa ttaaagcaga agttgctgca ttcaatgaag tagagattcc ttgctgtttg
     4201 acaacggaag gagataaagc gggttataaa gctgcgtcaa ttcttgtcga atttggaaag
     4261 cggttacctg aaggcgtttt taattcgatt agtgatttgg aaatggttac tcaaaagaca
     4321 gcagaaaaaa tgaacgaagg acaagtagta gcagaggtat atccacaaat tctcggcttg
     4381 aatcaaacgt tagaagaggc ttacatgtca actgttataa aaaat
//
LOCUS       DACEEZ010000141         4375 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20313_2017
            SAMN12639966-rid9879223.denovo.147, whole genome shotgun sequence.
ACCESSION   DACEEZ010000141 DACEEZ010000000
VERSION     DACEEZ010000141.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639966
            Sequence Read Archive: SRR10023158
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4375)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4375)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741683.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741683.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:44:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,016
            CDSs (total)                      :: 2,962
            Genes (coding)                    :: 2,889
            CDSs (with protein)               :: 2,889
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 36 of 73
            Pseudo Genes (incomplete)         :: 29 of 73
            Pseudo Genes (internal stop)      :: 18 of 73
            Pseudo Genes (multiple problems)  :: 8 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4375
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639966-rid9879223.denovo.147"
                     /isolate="E20313_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(77..1051)
                     /locus_tag="IWO89_002742"
     CDS             complement(77..1051)
                     /locus_tag="IWO89_002742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
                     /protein_id="HAQ0457433.1"
                     /translation="MSKKGTIINEWEKLTAPSNRRLKYQSLTLPLKMQWSIDRITIVG
                     KLKENIYYHTPNDVLILNFEQLMRLNEGNGYLRSVSNNGWQLLDQHEENIAYIEILKW
                     QEGKGRIDFNPNKISQFLASSMKNFIHDLFIEPHFSRADIACDIIDVPDEFITQYRVV
                     DPVSFKPIYGRSGKLETAYWGSRASERQIRMYNKKLEQERKRKIVPPEITTWWRLELQ
                     LRRGKATDWHEMVHESLDSFASPHYFPPNTKVTERIMVKGLLYDHSEWGNLAKNTKKK
                     YRDLLKQESQNDELTNHLRETFAESADDLKKELDTWLLGLDVTEEEEK"
     gene            complement(1500..2906)
                     /locus_tag="IWO89_002743"
     CDS             complement(1500..2906)
                     /locus_tag="IWO89_002743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase"
                     /protein_id="HAQ0457434.1"
                     /translation="MGFSISFKKGTKNTSISHNNRELTDSQKINDWHKHIDFTKSDEN
                     IYLEQTNIREKYEELFGEAVEKYNAKQKRADRKIEDYLAKVRKDKKLEPQREFIVQIG
                     TLDDFRTTRDDGFSTGISEQQAEQNRVIANKILVEYFKEFKERNPNLSVYNAVIHNDE
                     VSPHLHLNIVPVAEGYKRGVQKQPSFNKALQQQGLRADKENGRVIWNNFRNQEVESIE
                     RLMADFGWERELVGTNKIKDVHEYKEIMSEISELRQTAVKERESVSDELRYISRQREE
                     NEREKALVASERVKIEEEKKNMLNVKKEMAVIFQKDIVPLESFKRLPDGSYHLSGNDF
                     VDLYQRATRATNAKRNADNMSEQYLQMLEQNTELNEYVATLEEERHLSGRKQLADLQV
                     ENKKLKEENRSLRQRLERLKNAFQKSITRLSIRFGLAEKDMGLSEELEVLEQEKTLFY
                     RQNVSQRKLERSEDEIEW"
     gene            complement(3127..3489)
                     /locus_tag="IWO89_002744"
     CDS             complement(3127..3489)
                     /locus_tag="IWO89_002744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0457435.1"
                     /translation="MGMTLADIKAQEEKLKKEQAKLRQQKKRLIEKENAAMGKALRKR
                     FGFETFADFERWLSSISDDQQHEKSVDNEWLADEKSAPSERDYPDESDGLFGVIPNKK
                     SQMSESKFTSIFNDVNLR"
     gene            complement(3493..3801)
                     /locus_tag="IWO89_002745"
     CDS             complement(3493..3801)
                     /locus_tag="IWO89_002745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0457436.1"
                     /translation="MDKIDLREETVDRIELMYERLADYLNLPDEDNYSEMLERMMDVL
                     NSVINADLAKSSVLTNEFVHKHLAQTTDESEKEFWLKLMSHVMQLNQISVINSKEKEM
                     "
     gene            complement(3810..4229)
                     /locus_tag="IWO89_002746"
     CDS             complement(3810..4229)
                     /locus_tag="IWO89_002746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0457437.1"
                     /translation="MTDLSEKMTIKNLADELGVSKNTVRNQLVNSGFDLAQYKENGII
                     MLDKELIGVVRSIYRSKSDQLTGQLPVNNTGSDFPLEKENEFLRDQLAEKDKHISELT
                     EMIRDQQKLTLLQQQKNDQLLLENQELKNRKWWQFWK"
     gene            complement(4222..4362)
                     /locus_tag="IWO89_002747"
     CDS             complement(4222..4362)
                     /locus_tag="IWO89_002747"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0457438.1"
                     /translation="MSGKLLDVTQYELILKNNKIDSPIIIPKHAVKYIYAVAESSGEA
                     ND"
BASE COUNT         1236 a          975 c          626 g         1538 t
ORIGIN      
        1 aaaaaaacaa ccacaacgta atcacctgtt aaatttacca accaattttt cttaccaaaa
       61 tacttttgtt tttctgtcat ttttcttcct cctctgttac gtctagccct agaagccatg
      121 tgtctaattc tttttttaaa tcgtctgccg attccgcaaa ggtttctcgc aaatgattcg
      181 tcaattcatc attttgactt tcttgcttca acaaatccct gtattttttc tttgtattct
      241 tagctagatt tccccactca gaatgatcat aaagcaaacc tttcaccata atccgttcag
      301 taactttagt atttggcgga aaataatgtg gactagcaaa gctatccaaa ctttcatgaa
      361 ccatctcatg ccaatcagtt gcttttcccc ttcgtaactg caattctagt cgccaccatg
      421 tcgttatttc cggcggaaca attttacgtt ttctttcttg ttccagtttt ttattataca
      481 tccgaatttg tcgttcactg gcacgactac cccaatatgc cgtttctaac ttaccagaac
      541 gcccataaat tggtttaaaa gacacaggat caactacccg atattgtgta ataaactcat
      601 caggtacatc gataatgtcg caagcaatat ccgcacgtga aaagtgaggt tcgataaaca
      661 aatcatgaat aaaatttttc atggaactag ctaagaattg actaatctta ttcggattga
      721 agtctatacg ccctttcccc tcttgccatt ttaaaatttc aatataagca atattctctt
      781 catgttggtc taacaactgc caaccattat tactaacaga tcttaaataa ccattacctt
      841 catttaggcg cataagctgt tcaaaattta aaatcaacac atcatttgga gtgtgataat
      901 aaatattttc tttcagtttt ccaacgatag taattcggtc aattgaccac tgcattttta
      961 agggaagggt caaactttga tactttaacc gcctgttaga gggggctgtt aatttttccc
     1021 actcatttat gatcgtgcct tttttactca aattttgttc ctcctcaaag aaaaagagac
     1081 ttctggaaag aattttcaaa agttcacgta actcttaaaa attcattacc aaaaaatctc
     1141 tagtcttgaa gaaatctaaa aatattcgta taatgattaa gaagttacgg agctagtgcg
     1201 attagtttcc gtaaaggtcg agaacgttgg tagcgttgct cgactttatt tttttgctct
     1261 tttttcagtt tatttgtttg ctaactcaat aatagcactt ctctttagct atgcaaatat
     1321 ccaaaaaata aaaccgcacc ctttgaaaag ggcgccccca aacttcgcta acgctaggtt
     1381 ggccaaaaaa tggactggcc attttttagc aaatgtcagc ttcagggatt agtggtttag
     1441 ccgtcaactc cttatcctcg gcacaaaccg cctccggtac cgttgactgc acatcgtaac
     1501 taccactcga tctcatcttc agaacgctct aatttacgct gtgagacgtt ttgacgataa
     1561 aataaggttt tctcctgctc taacacttct aattcctcag aaagccccat atccttttct
     1621 gcaagaccaa aacggataga caaacgagtg atcgattttt gaaatgcgtt ttttaatcgc
     1681 tctaaacgct ggcgtaaaga gcgattttct tctttcagct ttttattttc cacttgtaaa
     1741 tctgctaatt gttttctacc agacaaatga cgctcttctt ccaatgtagc tacatactca
     1801 tttaattctg tattttgctc aagcatctgt aaatattgct cactcatgtt atcagcattt
     1861 ctcttggcat ttgttgctct cgtagctcgt tgatacaaat caacaaaatc attcccagat
     1921 aaatgatacg aaccatcagg aagcctcttg aaactttcta aaggaacaat atctttttgg
     1981 aaaatgaccg ccatctcttt ttttacattc agcatatttt tcttctcttc ttcaatcttc
     2041 actctctcag aagccaccag agccttttct cgctcatttt cttctctttg cctagaaata
     2101 tacctcaact catcagaaac gctctcacgc tcttttacgg ccgtttgacg caattctgaa
     2161 atctcgctca tgatttcttt atattcgtga acatctttga ttttgttagt accgactaac
     2221 tcacgctccc aaccaaaatc tgccatcaaa cgttcaattg actcaacttc ctgattccta
     2281 aaattattcc aaatcactcg accattctct ttatctgctc gtaaaccctg ctgttgcaag
     2341 gctttgttaa aacttggttg cttttgaaca ccccgtttat acccttcagc aacaggaaca
     2401 atattcaaat gcaggtgcgg acttacttcg tcattatgaa taacagcatt gtagacggat
     2461 aaattaggat tccgttcttt aaattctttg aaatattcaa ctaaaatttt gttggcaatc
     2521 actcgatttt gttctgcttg ttgttccgat atgccagtag aaaaaccatc atcacgagta
     2581 gtacgaaaat catcaagagt accaatttgc acgataaatt ctcgctgtgg ttccaatttt
     2641 ttatccttgc gaacctttgc taaatagtcc tcgatttttc tatcggctct tttctgcttt
     2701 gcattatatt tttctacggc ttcaccgaat aattcttcat atttttcacg aatatttgtt
     2761 tgttctaaat agatattttc atccgatttt gtgaaatcaa tatgcttatg ccagtcattg
     2821 attttttggc tatcagttaa ctcacgatta ttatgagaaa tgcttgtatt ctttgttccc
     2881 tttttgaacg aaatactgaa ccccaagtta aaaccctcct tcggctgtcg ctaataaaaa
     2941 gagtgtaaac ctttctgtta ctctttgtca aagagtaaca ttttcctaat tgtgacatgg
     3001 ctgtcacaat tccaaatgct ctccgagggt attcgctgtc acaagtgaca acgaaaaaag
     3061 gagccgaaag cgtgtcacgc tctcaactcc ttttacgctt tattttgctt cacgcaaaat
     3121 aatcgcttat cgtaaattca cgtcattaaa tattgacgta aatttactct cactcatctg
     3181 acttttctta ttcggtatca ctccgaataa gccgtcagat tcatcgggat aatctcgttc
     3241 gcttggcgct gatttttcat cagcaagcca ctcattatcc actgattttt cgtgttgctg
     3301 atcgtcagaa atcgacgata gccaccgctc aaaatcagca aaagtttcaa acccaaaacg
     3361 cttccgaaga gccttgccca ttgcagcatt ttctttctca atcaagcgtt ttttctgttg
     3421 gcgaagtttc gcttgttctt tttttaattt ttcttcttgc gctttaatat ctgctaaagt
     3481 cattcccata aactacatct ccttttcttt ggaattaata acggaaattt ggtttaactg
     3541 cataacatga ctcattaatt tcaaccaaaa ttctttttct gattcatcag ttgtttgcgc
     3601 taaatgctta tgaacaaatt cattagtcaa aactgaagat tttgctaaat cagcattgat
     3661 aaccgagttg agaacatcca tcattcgttc aagcatttca ctataattat cttcatccgg
     3721 caaattcaaa taatcagcta accgttcata catcaattca attctatcga ctgtttcttc
     3781 tctcaaatca attttatcca tgtcacacct cacttccaga attgccacca ttttcgattt
     3841 tttaattctt gattttctag taacaactga tcgttttttt gttgctgaag cagggtcaat
     3901 ttttgttgat cacgaatcat ttctgttaat tcagaaatat gtttgtcctt ctcagctaat
     3961 tgatcacgca aaaactcatt ctctttttct aaaggaaaat cactgcctgt gttattgacc
     4021 ggtaattgac cagtcaattg atcacttttt gaccgataaa ttgaccgaac aacaccaatc
     4081 aactctttat ctagcatgat aatgccattt tctttatact gagcaaggtc aaaccctgaa
     4141 ttgaccagtt gattgcgcac tgtatttttc gatacaccca attcgtcagc aagatttttt
     4201 atggtcatct tttctgacaa atcagtcatt agcttctcca ctactttcag ctaccgcata
     4261 aatatattta acggcgtgct tagggataat aatcggagag tcaattttat tatttttcaa
     4321 aatcaattca tattgcgtta catcaagtaa cttaccggac atttttttgc tatcc
//
LOCUS       DACEEZ010000142         4210 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20313_2017
            SAMN12639966-rid9879223.denovo.148, whole genome shotgun sequence.
ACCESSION   DACEEZ010000142 DACEEZ010000000
VERSION     DACEEZ010000142.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639966
            Sequence Read Archive: SRR10023158
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4210)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4210)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741683.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741683.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:44:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,016
            CDSs (total)                      :: 2,962
            Genes (coding)                    :: 2,889
            CDSs (with protein)               :: 2,889
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 36 of 73
            Pseudo Genes (incomplete)         :: 29 of 73
            Pseudo Genes (internal stop)      :: 18 of 73
            Pseudo Genes (multiple problems)  :: 8 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4210
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639966-rid9879223.denovo.148"
                     /isolate="E20313_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            <1..152
                     /locus_tag="IWO89_002748"
     CDS             <1..152
                     /locus_tag="IWO89_002748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAQ0457439.1"
                     /translation="NFAKNQGYNTILLGAQSTAETFYEKLGYTAYGDPFEDAGMPHIY
                     MKKTL"
     gene            complement(230..763)
                     /locus_tag="IWO89_002749"
     CDS             complement(230..763)
                     /locus_tag="IWO89_002749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF402 domain-containing protein"
                     /protein_id="HAQ0457440.1"
                     /translation="MGVPKEGEFVTIQSYKHDGKLHRTWRDTMVLKTSEHSMIGVNDH
                     TLVTESDGRRWVTREPAIVYFHKKYWFNVIAMIREKGVSYYCNLASPFLLDDEALKYI
                     DYDLDIKVFPDGEKRLLDVDEYEMHSKMMNYPNDIDFILKENVKILVDWINNGDGPFS
                     EGYIDIWYNRYKQLSRK"
     gene            complement(857..2035)
                     /gene="mutY"
                     /locus_tag="IWO89_002750"
     CDS             complement(857..2035)
                     /gene="mutY"
                     /locus_tag="IWO89_002750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="A/G-specific adenine glycosylase"
                     /protein_id="HAQ0457441.1"
                     /translation="MNKEWEKWTDKETKEFQDQFIQWYEQEKRNLPWRYNRDPYRIWI
                     SEIMLQQTRVDTVIDYFYRFMEWFPTIEELANAPEEKLLKAWEGLGYYSRARNIQAAA
                     KQIMSEFDGEMPQTPEEISSLKGIGPYTTGAIASIAFGLPEPAVDGNVMRVVSRLFCI
                     EADIAKASSRKIFDEAMRKIIDEKHPGEFNQAMMDLGSAICTPTSPKCETCPIQAFCL
                     ANKRGIQTSFPVKTKKAKPKDVYYISAALQNHSGAYYFEERDSQKLLANMWTFPMMEV
                     TQEEYERLKKEWETKPEIDLFDDLVAEEEQNLPFEKQELFVWQTRHLGEVTHIFSHLK
                     WHVLLFYGRATEGAEQEFTENKTSKWLKPDAFDSVVFPKVQMKLVDQLEKNRNNRNPF
                     "
     gene            complement(2028..2825)
                     /gene="recX"
                     /locus_tag="IWO89_002751"
     CDS             complement(2028..2825)
                     /gene="recX"
                     /locus_tag="IWO89_002751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination regulator RecX"
                     /protein_id="HAQ0457442.1"
                     /translation="MLTVTRISKGKGPFYRVDLSEGEALQVSEDVLVRYRLLKGRELD
                     EETIQEIKKSSGADFGFHLAMNYLSYQLRSEKEIRTYLKEKEILQEDRNKIVARLKEL
                     DLVDDLVYGESYVRTNMRLSDKGPKKLAQQMQQKGLKPEIIEQALSQYTFEDQVQNAH
                     QTAEKAFGKNHGKSQKELLRKIQQTLMTKGFSQEVIQQAIADLPQEADQEIEYDYLVK
                     QGDKLWRRNLRFEPKKRNLKVKQSLYQKGFDLDMIQRFITEKEEESE"
     gene            2932..>4210
                     /gene="rlmD"
                     /locus_tag="IWO89_002752"
     CDS             2932..>4210
                     /gene="rlmD"
                     /locus_tag="IWO89_002752"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HAQ0457443.1"
                     /translation="MAETIVKTGQTVPLKIKRLGINGEGIGYFKRLIIFVPYALPKEE
                     VLVKITKATPRYAEGELIKVKKTSKDRVVAPCPVYYECGGCQLQHLAYQAQLDFKKDL
                     LLQALEKFKPAGFRSYQLLPTIGMDEPWHYRNKAQFQLRKNKQTQKIEAGLYEANSHQ
                     LVPITDCLVQEPRTQKVMNTVVQLLNKFDAPIYDERTNSGIFRTIMVRIGVKTGELQV
                     VFITRSKKFPQKNAMIREINERLPEVVSIMQNVQAQKTSLVMGDETIHLWGKEAIEEQ
                     INEVTFDLSPRAFFQLNPQQTELLYGEGIKALDVQPNETIVDAYCGVGTIGLSVAKQA
                     KEVRGMDTVPQAIADAKKNAERLGYHNTRYEVGTAEQVLPKWLNEGFQPDGIIVDPPR
                     TGLDNALIQSLLKYPPKKLVYISCNVSTLARDLV"
BASE COUNT         1208 a          883 c          754 g         1365 t
ORIGIN      
        1 aaaactttgc taagaatcaa ggctataata cgattttatt aggggcacag tccacagctg
       61 aaacttttta cgaaaaacta ggatatacag cttacggcga tccttttgaa gatgctggca
      121 tgcctcatat ttatatgaaa aagactttat aaaaatttat ttcgaaaaaa gcaaaaaaca
      181 gcaagctttt gagaaaaatc tctcactggc ttactgttct tttttgtgtt tacttacgcg
      241 acaattgctt gtaacggtta taccaaatat caatatagcc ttccgagaac gggccatctc
      301 cattattgat ccagtcaacc agaatcttca cattttcttt taggatgaag tcaatatcgt
      361 tcgggtaatt catcattttg ctgtgcatct catattcatc gacatccaat agtcgtttct
      421 cgccgtcagg aaataccttg atatccaagt catagtcgat atatttcaag gcctcatcgt
      481 ccagcaaaaa aggtgaagct agattacaat aataggaaac ccccttttct ctgatcattg
      541 ctattacatt gaaccagtat tttttatgaa aataaacaat cgctggttcg cgcgttaccc
      601 atcggcgtcc atcagattca gtgactaatg tgtgatcatt cacgccgatc atagagtgtt
      661 cgcttgtttt taataccatg gtatctcgcc acgttcgatg caatttgccg tcgtgcttgt
      721 aactttggat cgtcacaaac tctccttctt ttggaactcc catagcaacc cttccttaag
      781 cagcattttg tgctttgtgt ttgggtgttc attacaccga catcataatt attatatcaa
      841 taaaaatgag ctttgcctag aaaggattcc tattgtttcg atttttctct aactgatcta
      901 ccagtttcat ttggacctta ggaaaaacga ccgaatcaaa tgcatcaggc ttcaaccact
      961 tcgaagtttt gttctcggta aattcttgtt ctgctccctc ggttgcccgt ccgtaaaaaa
     1021 ggagaacatg ccatttcaga tgactaaaaa tatgagtaac ttctcctaaa tgtcgtgtct
     1081 gccaaacaaa gagttcctgc ttttcaaatg ggagattttg ctcttcttct gctactaggt
     1141 catcaaacag atcgatttct ggctttgttt cccattcttt tttcaatcgc tcatactcct
     1201 cttgtgtcac ttccatcatt ggaaatgtcc acatattcgc cagcaatttt tggctatctc
     1261 tttcttcaaa ataatacgct ccagaatgat tttgaagagc tgcactgata taataaacgt
     1321 cttttggctt tgcttttttt gtttttacag gaaaactcgt ttggattcct cgtttgttag
     1381 ctaaacaaaa ggcttggatc ggacaagtct cacactttgg agaagttggt gtacagatag
     1441 ctgaacctag atccatcatt gcttggttga attcacctgg atgcttttca tcaatgattt
     1501 tccgcatcgc ttcatcaaag attttcctgc tagaagcttt tgcgatgtct gcttcaatgc
     1561 aaaacaatct gctgactact cgcatcacgt tgccgtcaac tgcaggttct ggaagtccaa
     1621 atgcgatact tgcaatcgcg cctgtcgtat atggtcctat tcccttcaat gagctaattt
     1681 cttcaggcgt ttgcggcatt tccccatcaa attctgacat gatctgttta gcagcagctt
     1741 ggatgttccg ggctcttgag taataaccaa ggccctccca tgcttttaaa agcttttctt
     1801 ctggagcatt tgctagctct tcaattgtag gaaaccattc catgaaacga taaaaataat
     1861 cgatcactgt gtctactctg gtttgctgaa gcatgatctc agagatccag atacgataag
     1921 gatcacgatt atagcgccaa ggcaggtttc ttttttcttg ttcataccat tgaatgaact
     1981 gatcttgaaa ttcttttgtt tctttatctg tccatttttc ccattcttta ttcactttcc
     2041 tcttcctttt ccgtaatgaa tcgctggatc atatccaaat caaatccttt ttgatataag
     2101 ctttgcttta cttttagatt tcttttttta ggttcaaacc ttaaatttct tcgccatagt
     2161 ttatctcctt gtttgactaa ataatcatat tctatttctt gatctgcttc ttgcggaaga
     2221 tcagctatcg cttgttggat cacttcttga gaaaagcctt tcgtcatcaa tgtttgttgg
     2281 atttttctca ataattcttt ttggcttttt ccgtgatttt ttccaaacgc tttttcggct
     2341 gtctgatgtg cattttgaac ttggtcttca aatgtatatt ggctgagcgc ttgttcaatg
     2401 atttcaggtt tcaaaccttt ttgctgcatc tgctgcgcta atttctttgg tcctttgtca
     2461 cttaagcgca tattcgtgcg cacataactt tccccataga ccaaatcatc gacaaggtcc
     2521 aattctttta agcgggctac gattttattt cgatcttctt gaaggatttc cttttctttt
     2581 aagtatgttc ggatttcttt ttccgatcgc aactgataac ttaagtaatt cattgctagg
     2641 tgaaaaccga aatcagctcc gctactcttc ttgatctctt ggatagtttc ttcgtctaat
     2701 tcccgtcctt ttaataatcg atagcgtact aatacatctt cagatacttg taaagcttct
     2761 ccttcagata aatctacgcg atagaaaggg cctttgcctt tactgattct cgtaacggtt
     2821 aacataaaca tcctcctcga acatatattc tctatcatac cacagaaaat ccccgtaatc
     2881 aggtattata tgttagaata aaccacagaa aaaagaaaaa gggagatact tatggcagaa
     2941 acaattgtca aaactggaca aacggttcct ttgaaaatca agcgtttagg cataaatggt
     3001 gaaggaatcg gttatttcaa acggttgatc atatttgttc catatgcact accaaaagaa
     3061 gaagtactag tgaaaatcac gaaagcaact cctcgctatg cagaaggaga attaataaaa
     3121 gtcaaaaaaa caagcaaaga tcgcgtagtg gcaccatgtc ctgtttacta tgaatgcggt
     3181 ggctgtcaat tacaacactt agcttatcaa gctcaattgg attttaaaaa ggatctgctt
     3241 ttgcaagcat tggaaaaatt caaaccagca ggctttcgat cttatcaact tttgccgacg
     3301 atcgggatgg atgagccttg gcattataga aacaaagcac aatttcagtt acgaaaaaat
     3361 aagcagactc aaaaaattga agctggttta tacgaagcaa actctcatca gttagtccct
     3421 ataacagact gcttggttca agaacctcga acacaaaaag taatgaatac tgttgttcaa
     3481 ctattgaata aatttgatgc acccatctat gatgaacgga caaacagtgg aatcttccgc
     3541 acaatcatgg ttagaatcgg tgttaaaact ggggagttac aagttgtttt tattactcgc
     3601 agtaaaaaat tcccgcaaaa aaacgcaatg atccgtgaga tcaatgaacg actgcccgaa
     3661 gttgtttcaa tcatgcaaaa tgtccaggca caaaaaacgt ctctcgtgat gggggatgaa
     3721 acgatccact tatggggaaa agaagccatt gaagaacaaa tcaatgaagt gactttcgat
     3781 ctttctcctc gtgccttctt tcaactcaat cctcagcaga ccgaattgct ttatggcgaa
     3841 ggaatcaaag cattagatgt ccagcctaat gaaacgatcg tggatgctta ttgcggagtt
     3901 gggacgatcg gcttaagtgt agcaaaacaa gccaaagaag tacggggaat ggataccgtt
     3961 cctcaagcaa tcgctgatgc aaaaaaaaat gcggaaagat taggatatca caacactcga
     4021 tatgaagtcg ggacagcaga gcaagttctg cctaaatggt tgaatgaagg ttttcagcct
     4081 gatggtatca tcgtagatcc accacgtacg ggtttggata acgcattgat ccaatcatta
     4141 ttgaaatacc ctccaaaaaa acttgtttat atctcctgta atgtttctac acttgccaga
     4201 gatctggtta
//
LOCUS       DACEEZ010000143         4210 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20313_2017
            SAMN12639966-rid9879223.denovo.149, whole genome shotgun sequence.
ACCESSION   DACEEZ010000143 DACEEZ010000000
VERSION     DACEEZ010000143.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639966
            Sequence Read Archive: SRR10023158
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4210)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4210)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741683.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741683.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:44:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,016
            CDSs (total)                      :: 2,962
            Genes (coding)                    :: 2,889
            CDSs (with protein)               :: 2,889
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 36 of 73
            Pseudo Genes (incomplete)         :: 29 of 73
            Pseudo Genes (internal stop)      :: 18 of 73
            Pseudo Genes (multiple problems)  :: 8 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4210
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639966-rid9879223.denovo.149"
                     /isolate="E20313_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            <1..1481
                     /locus_tag="IWO89_002753"
     CDS             <1..1481
                     /locus_tag="IWO89_002753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016617716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HAQ0457444.1"
                     /translation="SDSQGKDSVDLIRDSLFSIQVQQPWLLLQYNSSDIESIGADRVE
                     SLLSTSPDSNNGEDREKIVAEEIEDRNNVNMTITKTINRLGTVFFLFVFNIGISIFVF
                     LLTGIMIFSQVLFIIYAMFLPVSFILSMIPSFDGMSKRAIIKLFNTILTRAGITLIIT
                     TAFSISTMLYTLSAGYPFFLIAFLQIVTFAGIYFKLSDLMSMFSLQSNDSQSVGSRVM
                     RKPRMLMHAHMHRLQRKLGRSMTALGAGSAIASATGKQGQSGSSGRTQADHTRPDGQE
                     KSTLGKRIGQTIGAVADTKDKIVDSAGNLKEQVKDIPTNARYAVYQGKSKAKENVRDL
                     TSSISQTRADRASGRKEQQEQRRKTIAERRSEMEQVKQKKQPASSVHERPATKQEQSH
                     DGQTAKQSSIQTSRMESQQAKQERPAVKSDSLSLKAERQNRTTQERTVQKPATSTTPA
                     DRASQRPVTKERPSTVQRVQSQNLRNRPPIKSATIKKGSKKP"
     gene            1478..2479
                     /locus_tag="IWO89_002754"
     CDS             1478..2479
                     /locus_tag="IWO89_002754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018368130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase P60"
                     /protein_id="HAQ0457445.1"
                     /translation="MKLKALVIGGSGLFLMVFSLLLFVAILFSDEQDSGISNIHYGGV
                     NVSAEVLAHKPMVEKYAKEYGVEEYVNILLAIIQVESGGTAEDVMQSSESLGLPPNSL
                     STEESIKQGVKYFSELLASSERLSVDLESVIQSYNYGGGFLGYVANRGNKYTFELAQS
                     FSKEYSGGEKVSYPNPIAIPINGGWRYNYGNMFYVQLVTQYLVTTEFDDDTVQAIMDE
                     ALKYEGWRYVYGGASPTTSFDCSGLTQWTYGKAGINLPRTAQQQYDVTQHIPLSEAQA
                     GDLVFFHSTYNAGSYITHVGIYLGNNRMFHAGDPIGYADLTSPYWQQHLVGAGRIKQ"
     gene            2476..3408
                     /locus_tag="IWO89_002755"
     CDS             2476..3408
                     /locus_tag="IWO89_002755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015646598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HAQ0457446.1"
                     /translation="MRKEDLMMKFRKNQNKEKQIPKEKKPRVYKVNPHKKVVIALWVL
                     LGLSFSFAIFKHFTAIDTHTIHETTIIEKEYVDTHHVENFVENFAKVYYSWEQSDKSI
                     DNRMESLKGYLTDELQALNVDTVRKDIPVSSSVRGFQIWTVEPTGDNEFNVTYSVDQL
                     ITEGENTKTVHSAYIVSVYVDGSGNMVLVKNPTITNIPKKSSYKPKAIESEGTVDSIT
                     TNEINEFLTTFFKLYPTATASELSYYVNDGILKPIGKEYIFQELVNPIHNRKDNQVTV
                     SLTVEYIDQQTKATQVSQFDLVLEKNGSNWKIVK"
     gene            3653..3769
                     /locus_tag="IWO89_002756"
     CDS             3653..3769
                     /locus_tag="IWO89_002756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001814923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance determinant leader
                     peptide"
                     /protein_id="HAQ0457447.1"
                     /translation="MILKYPENICMLCIPMVMHKNPSDKSIYHWDFYALLGF"
     gene            3785..>4210
                     /locus_tag="IWO89_002757"
     CDS             3785..>4210
                     /locus_tag="IWO89_002757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019117172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HAQ0457448.1"
                     /translation="MKIINIGVLAHVDAGKTTLTESLLYNSGAITELGSVDKGTTRTD
                     NTLLERQRGITIQTGITSFQWENTKVNIIDTPGHMDFLAEVYRSLSVLDGAILLISAK
                     DGVQAQTRILFHALRKMGIPTIFFINKIDQNGIDLSTVYQ"
BASE COUNT         1387 a          800 c          869 g         1154 t
ORIGIN      
        1 attccgatag tcaaggcaag gacagcgtgg acttaatcag agatagcctg ttttccatac
       61 aggttcagca accgtggcta ctgcttcaat acaacagttc agacattgaa agtattggtg
      121 ctgaccgtgt ggaaagtcta ctttcaacca gtccagacag taataatgga gaagacagag
      181 aaaaaattgt agcagaagaa atcgaagaca gaaacaatgt gaacatgacg attacaaaaa
      241 ctattaaccg tttgggtaca gtcttctttc tctttgtatt caatatcggg atttctatat
      301 tcgtattcct actaacagga atcatgattt tctcgcaggt actttttatc atctatgcca
      361 tgtttctgcc tgtgagcttt attttaagca tgattccgtc ctttgatggt atgtcaaaac
      421 gagccattat caaactcttt aataccattt tgacacgagc tggaatcacg ctgattatta
      481 cgacagcctt tagtatttca accatgctct ataccttatc ggctggttat ccgttctttt
      541 tgattgcctt tttacagatt gtgacctttg caggaatcta cttcaaactg agcgatttaa
      601 tgagtatgtt ttcgcttcaa agcaacgatt ctcaaagtgt gggaagtcgt gtgatgagaa
      661 aacctcgtat gcttatgcac gctcatatgc accgcctaca gcgtaaactt ggacgctcca
      721 tgacagcttt aggtgctggc tctgccattg ccagtgctac aggaaaacaa ggacagtcgg
      781 gtagttctgg taggacacaa gcagatcaca cccgtccaga cggacaggaa aaatcaacac
      841 ttggaaaacg tatcggacaa acaatcggag cagttgctga taccaaagat aaaatcgttg
      901 attctgctgg taatctcaaa gaacaggtta aggatattcc gaccaatgca agatatgcag
      961 tgtatcaagg aaaatccaaa gcaaaagaaa atgttcgtga cctaacttcc agtatttctc
     1021 aaaccagagc agacagagcc agcggacgca aggaacagca ggaacaaaga cgcaaaacca
     1081 ttgcagagcg tcgctctgaa atggaacagg tcaaacagaa aaaacagcca gcttcttccg
     1141 ttcatgaaag accagctact aagcaagaac aatctcatga tggacagacc gcaaaacaat
     1201 cttctattca gacttcacgc atggaatctc aacaagccaa acaggagcgt ccagcagtta
     1261 agtccgattc tttaagttta aaagcggaac gacaaaaccg tactacacaa gaaagaacag
     1321 ttcaaaagcc agcaacttca actacaccag cagacagagc ttcacaacgt ccagtcacaa
     1381 aagagcgtcc gtctactgtt caaagagttc aaagccaaaa ccttagaaat agaccaccaa
     1441 taaaatccgc cactatcaag aaaggcagta agaaaccatg aaactgaaag ctttagtgat
     1501 tggtggttct gggctattct tgatggtctt ctcactgctt ctgtttgttg ccattttatt
     1561 ttcagatgaa caggacagcg gaatttccaa tattcattat ggaggtgtga atgtttccgc
     1621 agaagtgctg gctcataagc ctatggtaga aaaatatgcc aaagaatatg gcgttgaaga
     1681 atatgtcaac atacttcttg cgattataca ggtggaatcg ggcggtactg cggaagatgt
     1741 tatgcagtcc tcggaatccc tcggtcttcc acctaattca ttgagtacag aagaatccat
     1801 taagcaaggt gtgaagtatt tcagtgaatt attagccagt agcgaaaggc tcagtgtaga
     1861 tttagaatcg gttatccagt cctacaatta tggtggtggt ttcttagggt atgtggctaa
     1921 tcgtggaaat aaatatacct ttgaactggc tcaaagtttc tcaaaagagt attcaggtgg
     1981 cgaaaaagtg tcttacccca atcccatagc catacctatc aatgggggct ggcgatacaa
     2041 ctatggcaat atgttttatg tgcaactggt aacgcagtat cttgtcacaa cagagtttga
     2101 tgatgatacg gtacaagcca tcatggacga agcactgaaa tatgagggct ggcgatacgt
     2161 ttacggtgga gcttccccga ctacttcttt tgattgtagc ggactgacac aatggacgta
     2221 tggaaaagct ggaattaact taccacgaac cgcacaacag caatatgatg tgacccagca
     2281 tatcccacta tcggaagcac aagctggcga tttggttttc tttcattcta cctataacgc
     2341 tggctcttat attactcatg ttgggatata ccttggcaat aaccgtatgt ttcatgcagg
     2401 cgacccaatc ggttatgccg acttaacaag cccctactgg caacagcatt tagtgggagc
     2461 aggacgaatc aaacaatgag aaaggaagat ttaatgatga aatttagaaa aaatcagaat
     2521 aaagaaaaac agataccaaa ggaaaagaaa cctcgtgtct ataaggtcaa tcctcataaa
     2581 aaggttgtga ttgccttgtg ggtactttta gggcttagtt tcagctttgc gatattcaag
     2641 cactttacag ctatagatac tcatactatt cacgaaacaa ctatcataga aaaggaatac
     2701 gttgatactc atcatgtaga aaattttgta gagaactttg cgaaagtcta ctattcatgg
     2761 gagcaatccg ataagtccat tgataatcga atggaaagtc taaaaggcta tctgacagat
     2821 gaacttcaag ctctcaatgt tgatacagta cgcaaagata ttcctgtatc gtcttctgta
     2881 agaggatttc agatatggac ggtagagcca actggcgaca atgagtttaa tgtaacctac
     2941 agtgtagacc agctcattac agagggagaa aatacaaaga ccgtccactc tgcttatata
     3001 gtgagtgtct atgtagatgg ttctggaaat atggtactgg ttaagaatcc gaccattacc
     3061 aacataccta agaaatcaag ttataaacca aaagccattg aaagtgaggg gacggttgat
     3121 tccattacaa ccaatgaaat caatgagttt ttaacgacgt tcttcaagct ctatcctaca
     3181 gcgacagcca gtgaactttc ctactatgtg aatgacggga tattaaaacc aatcggaaaa
     3241 gagtacatct ttcaagaact ggtaaatcct attcacaatc gtaaggataa tcaagtcacg
     3301 gtatcgctga cagtggagta tatcgaccag cagaccaaag caacgcaggt atctcaattt
     3361 gatttggtac ttgaaaagaa cgggagtaat tggaagattg taaaataaca aatattggta
     3421 catgattaca gatactttgt aatcatgtac tctttttgat aaaaaattgg agattccttt
     3481 acaaatatgc tcttacgtgc tattatttaa gtgactattt aaaaggagtt aataaatatg
     3541 cggcaaggta ttcttaaata aactgtcaat ttgatagcag gaacaaataa ttagatgtcc
     3601 ttttttagga gggcttagtt ttttgtaccc agtttaagaa tacctttatc atgtgattct
     3661 aaagtatcca gagaatatct gtatgctttg tatacctatg gttatgcata aaaatcccag
     3721 tgataaaagt atttatcact gggattttta tgcccttttg ggtttttgaa tggaggaaaa
     3781 tcacatgaaa attattaata ttggagtttt agctcatgtt gatgcaggaa aaactacctt
     3841 aacagaaagc ttattatata acagtggagc gattacagaa ttaggaagcg tggacaaagg
     3901 tacaacgagg acggataata cgcttttaga acgtcagaga ggaattacaa ttcagacagg
     3961 aataacctct tttcagtggg aaaatacgaa ggtgaacatc atagacacgc caggacatat
     4021 ggatttctta gcagaagtat atcgttcatt atcagtttta gatggggcaa ttctactgat
     4081 ttctgcaaaa gatggcgtac aagcacaaac tcgtatatta tttcatgcac ttaggaaaat
     4141 ggggattccc acaatctttt ttatcaataa gattgaccaa aatggaattg atttatcaac
     4201 ggtttatcag
//
LOCUS       DACEEZ010000144         4203 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20313_2017
            SAMN12639966-rid9879223.denovo.150, whole genome shotgun sequence.
ACCESSION   DACEEZ010000144 DACEEZ010000000
VERSION     DACEEZ010000144.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639966
            Sequence Read Archive: SRR10023158
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4203)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4203)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741683.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741683.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:44:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,016
            CDSs (total)                      :: 2,962
            Genes (coding)                    :: 2,889
            CDSs (with protein)               :: 2,889
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 36 of 73
            Pseudo Genes (incomplete)         :: 29 of 73
            Pseudo Genes (internal stop)      :: 18 of 73
            Pseudo Genes (multiple problems)  :: 8 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4203
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639966-rid9879223.denovo.150"
                     /isolate="E20313_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            <1..305
                     /locus_tag="IWO89_002758"
     CDS             <1..305
                     /locus_tag="IWO89_002758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="D-alanyl-lipoteichoic acid biosynthesis protein
                     DltD"
                     /protein_id="HAQ0457449.1"
                     /translation="EKWSDYTGLSQEMLQGFAKKIKFQLNSQGFNRIADFVNQAGTNY
                     FMEDTIHLGWKGWLAADQQIRPFLEENHITASKYHLDDAFFSKSWQHQIPDKLQLK"
     gene            446..1528
                     /locus_tag="IWO89_002759"
     CDS             446..1528
                     /locus_tag="IWO89_002759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M42 family metallopeptidase"
                     /protein_id="HAQ0457450.1"
                     /translation="MDTKEEQLLIDLTSAKGVPGNEEEVREVFREYAKPFADDIFYDG
                     LGSVIAKHGAGGGPKVFISGHMDEVGFMVTKITEKGFLEFQTLGGWWGQVMLAQQVEI
                     KTREGKIVHGVIGSKPPHVLTPQVRNKPYEIKDMFIDIGASSQEEAKEWGIRPGDMVT
                     PYIEYKRMNGSKYLLAKAWDNRIGTAVSLRVLENLSKEAHPNVLFAGSDVQEEVGLRG
                     ARTSTHLVNPDIAFALDTGTAGDTPGMTPKEADSILGKGPQILIFDASMIPHKKLLNF
                     VIDVAEEMDIPFQYTVISGGGTDAGQMHLTRDGVPSVAITVPVRYLHSHTSVIHEDDY
                     LNTVKLVTELVKRLDSETVEKIRSYE"
     gene            1528..2331
                     /locus_tag="IWO89_002760"
     CDS             1528..2331
                     /locus_tag="IWO89_002760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF975 family protein"
                     /protein_id="HAQ0457451.1"
                     /translation="MNNRIPISEYRKKARVSLENQWGINTWLVFLSIIFTSVIQNFFD
                     GFSPFPEESMQQHLLSFLVDNLLLFAFNYALFYIALVVVRGGRPKSNLLFSVFQRKYF
                     GSILGINLINSVINWLLNSLIFLPMFLISGVSTYMQLLFSSNAVPTGVMPEDFLDISF
                     VLSMIGMIFVSALIMSIVGGLFQFAAWTKFDYPDLSVIQCLKYGWFLLKDRIGTYILL
                     QLSFIGWYLLGAIALFIGLLWVIVYVNVSVAEFYEQARIEKGGPDAYFT"
     gene            2401..3141
                     /locus_tag="IWO89_002761"
     CDS             2401..3141
                     /locus_tag="IWO89_002761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HAQ0457452.1"
                     /translation="MKLTVLGCLGAYPYKNQGTTGYLLQSDGFNLLIDAGSATLIKLQ
                     EHLDPLDLDAVILSHYHHDHIADLGVLQYYWQLHPKRNPETLLSIYGHTKDSIHFEEL
                     TLENVSKGHPYFEAEELKIGPFLITFMETIHPVVCYAMRIVEETTGKVFVFTGDSGYL
                     ESFADFAKDADLFLADTYLFEGNEHHHAHFTSKEAGELAKSANVKKLVLTHLPQFGDL
                     EQLKNEAQKAAGKGIDIELAAVDKVFNI"
     gene            3152..4156
                     /locus_tag="IWO89_002762"
     CDS             3152..4156
                     /locus_tag="IWO89_002762"
                     /EC_number="6.3.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase"
                     /protein_id="HAQ0457453.1"
                     /translation="MIFVPNENHDPRINLAIEQYLLQEMPLDEPILLFYINEPSIIIG
                     RNQNTLEEINRDYVEENGIHVVRRLSGGGAVYHDFGNLNFSFIMPDDGDSFRDFAKVT
                     QPIIQALHELGVAGAELKGRNDLVIDGMKFSGNAMYATNGRMFAHGTIMFDSDINEVV
                     NALKVKKDKIESKGIKSIRSRVTNIKPFLPEEKQKMTTEEFRQEILLKIFGVESVEEV
                     KTYELTDEDWKKINRISEEYYRNWDWNYGKSPDFNFSRQKRFSIGSIEVHLNVSEGMI
                     KEAKIFGDFFGLGDISDVEKKLVGQKYDKASLSKIVSQIDVKKYFGAIEPEELLTLLY
                     "
BASE COUNT         1376 a          688 c          876 g         1263 t
ORIGIN      
        1 acgagaaatg gagcgattat accggactat ctcaagaaat gttacaagga tttgccaaaa
       61 agatcaaatt tcaattaaat agccaaggat tcaatcgaat agctgatttt gtcaatcaag
      121 cgggtaccaa ctattttatg gaagacacga tccatctagg ctggaaaggc tggttggctg
      181 cagatcaaca gatcagaccg tttttagaag aaaatcatat tactgctagt aagtatcatc
      241 ttgacgatgc tttttttagc aaatcttggc aacatcaaat accagataaa ctacaattaa
      301 aataaagaaa acaggcgagt gttgctcgct tgttttcttt atttttgaat attctgaata
      361 aaagataaaa ataccaagtt tgcctgaaca aaaatcataa gcaatggtat aatcacaata
      421 tgaatataga aatgggggaa aacatttgga tacaaaagaa gaacagctac ttattgattt
      481 aacttctgca aaaggagtac ctggaaatga agaagaggta agagaagtat tcagagaata
      541 tgccaaacca ttcgctgatg atatttttta tgacggttta ggcagtgtga ttgccaaaca
      601 tggtgctggc ggaggcccca aggtctttat ttctggacat atggacgaag ttggttttat
      661 ggtaacaaaa ataaccgaaa aaggattctt ggaattccaa actcttggag gttggtgggg
      721 gcaagtcatg cttgctcagc aagttgaaat caaaacacgt gaagggaaaa tcgtccacgg
      781 tgtgattggt tccaaaccac cacatgtttt aacacctcaa gtaagaaata aaccttatga
      841 aataaaggat atgttcattg atattggcgc atcaagccaa gaagaagcca aagagtgggg
      901 catacgtcct ggagatatgg tcactccata tattgaatac aaacgaatga atggcagtaa
      961 atatttactg gcaaaagcat gggataatcg tatcgggaca gctgtttctc tgcgagtatt
     1021 agagaatcta tctaaagaag cacacccaaa tgttttattc gcaggaagcg atgtgcaaga
     1081 agaagtaggc ttgcgaggtg ctcgaacaag tacacacctt gtaaatcccg atattgcttt
     1141 tgctttagat actggtacag caggagacac tccaggaatg acaccaaaag aagcagattc
     1201 gattttagga aaaggcccac agattttgat ttttgatgca tctatgatcc cgcataaaaa
     1261 attattgaat tttgttattg atgtagcaga ggaaatggat attccattcc aatatacagt
     1321 gatttctggc ggaggaactg atgcaggaca aatgcatttg acaagagatg gtgttccgtc
     1381 tgtagccatt accgttcctg ttcgttatct gcattcccat acatcagtga ttcatgaaga
     1441 tgattatttg aatacagtga aactagtcac agaattagtg aaacggcttg atagtgaaac
     1501 agttgaaaaa attcggagtt atgaataatg aataatagaa tacctatttc tgaatatcgg
     1561 aaaaaagcac gagtttcttt ggaaaatcaa tggggaatca atacttggct tgttttctta
     1621 tctataatat ttacgagcgt catccagaac ttttttgacg gtttcagtcc ttttccggaa
     1681 gaaagcatgc agcagcactt attgagtttt ctagtcgata atttactttt attcgcattc
     1741 aactatgcat tgttttacat tgcgctagta gttgtacggg gtggcagacc aaaaagtaac
     1801 cttttatttt cagttttcca aagaaagtat tttggttcga tacttggcat caatttgatc
     1861 aattcagtca tcaactggct actcaacagt ttaatctttc tgcccatgtt tttaatcagc
     1921 ggagtgagta cttatatgca gctgcttttc agttcaaatg cggtacctac gggtgtcatg
     1981 ccagaagatt ttcttgatat cagctttgtt ttatcgatga tagggatgat ttttgtttca
     2041 gcactcatca tgagtattgt tggcggttta ttccaatttg ctgcatggac aaagttcgac
     2101 tatcctgatt tgtcggttat tcaatgtttg aaatatggct ggtttttatt gaaagataga
     2161 attggtacgt atattttgtt gcagctgtct tttatcggct ggtatttgct aggagcaatc
     2221 gctttgttca tcggtctttt gtgggtcatt gtttatgtga atgtgtcagt tgcagaattt
     2281 tatgaacaag cacggattga aaaaggaggt ccagacgcct attttactta gcatgtaagc
     2341 agtttattca tcaaaaaaat aacttttgtg gtaggataaa tctttgaggg ggaatgtacg
     2401 atgaaattaa ctgttttagg atgcttagga gcgtatcctt ataaaaatca agggacaaca
     2461 ggttatttgc ttcaatctga cgggttcaat ttgttgatcg atgcaggtag tgctacgctg
     2521 atcaaattac aagaacacct tgatccactt gatctagatg ctgtgatcct tagccattat
     2581 catcatgatc atattgctga ccttggtgta ttacagtatt actggcaact tcatcctaag
     2641 agaaatccgg agacattgct ttcaatttat ggacacacaa aagattccat tcactttgaa
     2701 gaattgacgt tggaaaatgt ctcaaaagga catccttatt ttgaagctga agaattaaaa
     2761 attggtcctt ttctgattac ttttatggaa acaatccatc cagtagtatg ttacgccatg
     2821 cgtatcgtag aagaaacaac ggggaaagta ttcgtattta caggtgattc aggttatttg
     2881 gaaagtttcg ctgattttgc gaaagatgct gatttgtttc tagcagatac ctatcttttt
     2941 gaaggaaatg aacatcatca tgcccatttc acttcaaaag aagctggaga acttgcgaaa
     3001 tcagctaacg taaagaaact tgtacttaca cacctacctc agtttggtga tttagaacaa
     3061 ttgaaaaatg aagcgcaaaa ggcagcaggc aaagggattg atatagaact tgctgcagtt
     3121 gacaaagtgt ttaacattta ggaggaagaa catgattttt gtaccaaatg aaaaccacga
     3181 cccaagaatc aatttagcaa ttgaacaata tttgctgcaa gaaatgcctt tagatgaacc
     3241 tattttgctc ttttatatca atgaaccttc aatcatcatc ggcagaaacc aaaatacgct
     3301 tgaagaaatc aatcgagatt atgtggaaga aaatggcatt catgttgtcc gtcgcttaag
     3361 tggaggcgga gctgtctacc atgattttgg aaacctgaac tttagtttta tcatgcctga
     3421 tgatggggat tcattccgtg actttgcgaa agtaacacag ccaatcatcc aagcacttca
     3481 tgaattgggt gttgctgggg cggaattgaa aggccgaaat gatctagtca ttgatggcat
     3541 gaaattttct ggcaacgcga tgtatgctac caacggtcgg atgtttgctc atggaacaat
     3601 tatgttcgac tctgatatca atgaagtagt caatgcatta aaagtaaaaa aagacaaaat
     3661 cgaatcaaaa ggaatcaaat cgatccgttc tcgtgtaacg aatatcaaac catttcttcc
     3721 agaagaaaag caaaaaatga caacagaaga atttcgtcaa gaaatcttgt tgaaaatttt
     3781 cggcgtagag tcagtagaag aagtaaaaac gtacgaattg actgatgaag attggaaaaa
     3841 aatcaatcga atctctgagg aatattatcg aaattgggat tggaattacg ggaagtctcc
     3901 agatttcaat ttctctcgcc aaaaacgttt ttctatcggt tcgattgaag tccatttgaa
     3961 tgtctcagaa ggaatgatca aagaagcaaa aatctttggc gatttcttcg gactaggaga
     4021 tatctctgac gtggaaaaaa aattggttgg acagaaatat gataaagcat ctctatcaaa
     4081 aatagtcagc caaattgatg tgaagaaata ttttggtgcg atagaaccag aagaattgct
     4141 taccttactt tattgaaaat aaaaaatagc ggaatccaca tatatggatt ccgctatttt
     4201 tta
//
LOCUS       DACEEZ010000145         4202 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20313_2017
            SAMN12639966-rid9879223.denovo.151, whole genome shotgun sequence.
ACCESSION   DACEEZ010000145 DACEEZ010000000
VERSION     DACEEZ010000145.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639966
            Sequence Read Archive: SRR10023158
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4202)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4202)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741683.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741683.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:44:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,016
            CDSs (total)                      :: 2,962
            Genes (coding)                    :: 2,889
            CDSs (with protein)               :: 2,889
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 36 of 73
            Pseudo Genes (incomplete)         :: 29 of 73
            Pseudo Genes (internal stop)      :: 18 of 73
            Pseudo Genes (multiple problems)  :: 8 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4202
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639966-rid9879223.denovo.151"
                     /isolate="E20313_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(1..353)
                     /locus_tag="IWO89_002763"
                     /pseudo
     CDS             complement(1..353)
                     /locus_tag="IWO89_002763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348804.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(613..1161)
                     /locus_tag="IWO89_002764"
     CDS             complement(613..1161)
                     /locus_tag="IWO89_002764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0457454.1"
                     /translation="MADIKGLDIFGNNKGLAKIKKEAEAKKKQQAITPSENKQLEVHQ
                     VQEIKTSSNVERQSRGRTKKVGAPIRKFDKVYSTKHPLKLSTLLNATSRVLVEKYETN
                     MTRDELLRKALDEYIKQNLTQEDKQDLFNDVVKELDLFREKYPIVPELDDRGEIIRTT
                     SQIKQDTEDSLRKGWGMNNKVR"
     gene            complement(1162..2019)
                     /locus_tag="IWO89_002765"
     CDS             complement(1162..2019)
                     /locus_tag="IWO89_002765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HAQ0457455.1"
                     /translation="MVAKKYVIGNFKGGVGKSTCAQMLGFESAVNEGKRTLIIDLDMQ
                     GNTSDVMNLTHMNFTNEEGGGEGEPIVYENTVTDVLINNLPPKEAVYKVINNLYILPA
                     DMSFEMYDDWIKDQYNDSLSQFKYMKEKLEPLFDEFDVIYLDVPPSISVYSKSAMYIA
                     DWAIVVLQTQVKSMRNAMQYLEYMDFFVKEFDTNLYVAGVIPFMLESGDAVDQEMYKQ
                     AQEIYGEHLIKNVVLKNARLKRYDGSGITMEKTKKGLLKQWDRKSHELFISILKELEE
                     HENWYAEVE"
     gene            complement(2305..2591)
                     /locus_tag="IWO89_002766"
                     /pseudo
     CDS             complement(2305..2591)
                     /locus_tag="IWO89_002766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292679.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     gene            complement(2581..2910)
                     /locus_tag="IWO89_002767"
     CDS             complement(2581..2910)
                     /locus_tag="IWO89_002767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HAQ0457456.1"
                     /translation="MITKTRKQGNSIMLTVPKDFNVPNGVEVEAKLVENGILYEFVEP
                     QKEFYDFSEDILSDIIAEGYDKDEILVEFKNRKNKMHSSFRDIAEDTLTNSKVMTKEE
                     LATEIGL"
     gene            complement(2987..3259)
                     /locus_tag="IWO89_002768"
     CDS             complement(2987..3259)
                     /locus_tag="IWO89_002768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Txe/YoeB family addiction module toxin"
                     /protein_id="HAQ0457457.1"
                     /translation="MNNYSVMIKNSAKADLKKIKQSNLKPQFEKVIQTLKEDPYLPTQ
                     SFEKLKPTHEGRYSRRLNRQHRVVYKVDEDEHVVEIYSARTHYESQ"
     gene            complement(3259..3522)
                     /locus_tag="IWO89_002769"
     CDS             complement(3259..3522)
                     /locus_tag="IWO89_002769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HAQ0457458.1"
                     /translation="MAKTVLNPSSARKNFYKLLKDVNENHTEIEIISERSENNAVLIG
                     LDDWKAIKETLLLEQIGTLGVVRKREKDDSGFTNVDEIDWDNL"
     gene            complement(3628..4044)
                     /locus_tag="IWO89_002770"
     CDS             complement(3628..4044)
                     /locus_tag="IWO89_002770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0457459.1"
                     /translation="MEFREGAFDFQTSQQNRKFIPEDFQNLQLENMRLEDLFRLADDD
                     KYRFEVLKIKKNELLNKLYPENAIKAHFGDANHMYSWFIPKRFRGKINAGDQVKVLNR
                     NSTDVVMITDIYVEDEVIIKYMKPVIKLVNKNKGNG"
     gene            complement(4050..>4202)
                     /locus_tag="IWO89_002771"
     CDS             complement(4050..>4202)
                     /locus_tag="IWO89_002771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS6 family transposase"
                     /protein_id="HAQ0457460.1"
                     /translation="NKFYQSLRTASSTIKGMETIRGIYKKNRRNGTLFSFSVSTEIKV
                     LMGITA"
BASE COUNT         1225 a          851 c          533 g         1593 t
ORIGIN      
        1 aaatgttctt actgcctctt ccacactaat ttcttcgttc aatttcacta ataatacttg
       61 atctcgctta ttataatcgc tttcatttat cctaagagtc aaatcttttt ccaccataac
      121 ttttacaccc aataaatttt gcggtttatt tttctctgtt gaaattcttt gtgtagcctt
      181 taaactgaca attagctcat tgggtacata cttatttgag agtcatctaa ttggctcata
      241 atcctttctt tttggttagt atctgcagtt acttgtttag tatgagccat gaacccaaac
      301 atactcaccc caacaaccaa tcctaaatat attttctttt taaacttttt catctacatc
      361 tcctcctaat agttttattt tagagttagc tctattactt atttactaac tcaacaacag
      421 attatcatac acttgttaat gaaatcacaa tatctcatta aaacacaccc taaaatcata
      481 accgttccta tgctcatttt ttggattttt taatcacgta accagaaaaa taaatcccct
      541 ctatttcacg caaatcaaag aagaactaag taactaccgt ttaaaggtag ttacttagtt
      601 cttctttgat tattatctaa ctttattatt cattccccac cctttgcgca gtgagtcttc
      661 ggtatcttgt tttatctgac tggtagttct tattatttct ccacgatcat ctaattctgg
      721 aacaattgga tatttttctc tgaacaagtc tagttctttc accacgtcat taaacaaatc
      781 ttgcttatct tcttgcgtaa gattctgttt aatatactca tctaaagctt ttctaagcaa
      841 ttcatctctt gtcatattag tctcatactt ttctactaaa actctggaag tcgcatttaa
      901 caaagtcgac agttttaaag gatgcttggt ggaataaacc ttatcaaact ttctgattgg
      961 tgcgccaact ttttttgttc ttcctctact ctgtctttca acattggagg aagtttttat
     1021 ttcttgaact tggtgcactt ctaattgctt attttcagat ggtgtaattg cttgctgctt
     1081 tttctttgct tctgcttctt tttttatttt ggcgagtcct ttgttgttcc caaaaatatc
     1141 aagtcctttg atgtcagcca attactccac ctccgcatac caattctcgt gttcttccaa
     1201 ttctttcaaa atactgataa ataattcgtg gcttttgcga tcccattgtt ttaagagacc
     1261 tttctttgtc ttctccatcg taatacctga tccgtcgtat cgttttaagc gcgcattttt
     1321 gagtacaaca tttttaatca aatgttcccc ataaatctct tgggcttgtt tatacatttc
     1381 ctgatctact gcgtcaccgc tttccaacat gaaaggaata acaccagcaa catataaatt
     1441 tgtatcaaat tctttcacaa aaaagtccat atactcaaga tattgcatcg catttctcat
     1501 cgattttact tgggtctgta agacgacgat tgcccaatcc gcaatgtaca tcgcggattt
     1561 actatataca gaaatagaag gtgggacatc taaatagatg acgtcaaact catcaaataa
     1621 tggttctagc ttttctttca tatatttgaa ttgggataat gaatcattat attggtcttt
     1681 gatccaatca tcatacattt caaaggacat gtctgctgga agtatataga ggttattgat
     1741 tactttatag acagcctctt taggtggaag attgtttatc aacacgtcag tcacagtatt
     1801 ttcgtaaaca atcggttcac cttctccgcc tccttcttcg ttagtgaagt tcatatgcgt
     1861 taagttcatc acatcagaag tatttccttg catatccaag tcaattatca aggttctttt
     1921 tccttcatta actgctgatt caaaccctaa catttgcgcg cacgtacttt tacctacgcc
     1981 acctttaaaa ttaccgatta catatttctt cgctaccacg ttcctacctc ttttctttta
     2041 gcaatattca tttgatacta ttataacata tttattttta gttactacca aaaaaaagat
     2101 atatatgtac taccttataa ttaataagta aaaggtagta cataaataag gaaaagctga
     2161 aatatattga tttatagctt tcctcaaagt aatgacgtta tttaatttat cgagaagcag
     2221 agaatactcg cgactttagt catgtagacg aatcagattt tcgataaaaa agaagccgtc
     2281 cttcttaaga acgacaacaa atcactagat taattttttc aactgttcat aaaagttttc
     2341 atgcgttcca cataacaata ctggaataac cttgttttct ataatctcgt aagctactct
     2401 atatgttgtg cccgcatatt tgaacccact agaccaaata cctgataaat ccccagtttt
     2461 ttgttcaccc ttatatggat caatcgcaat ttcatcataa attaaattca aaaatttttc
     2521 tttcaatggc ttctctttta gctttttaaa tacttaatga catgttttga aggaacgatt
     2581 tcataagcca atttctgttg ccaactcctc tttagtcatt actttagaat tagtaagtgt
     2641 atcttcagca atatctctaa aggaagaatg cattttgttt tttctattct taaattctac
     2701 aagaatctca tctttatcgt agccttctgc aattatatca gacaaaatat cttcgctaaa
     2761 atcataaaat tctttttgtg gttcaacaaa ttcataaaga ataccattct ctaccaactt
     2821 tgcttcaact tcgactccat taggaacatt aaaatcttta ggtactgtca acatgattga
     2881 attgccttgt tttcttgttt ttgtaatcat ttaaatccct catttctaac agtatgtaat
     2941 tacttagtaa atacattata ccacgagata atgtatttac aagtaattac tgtgactcgt
     3001 aatgcgtcct tgctgaataa atttcaacca cgtgttcatc ttcatctacc ttataaacaa
     3061 cccggtgttg tctattcaat cttctagaat acctaccttc atgtgtaggt tttaacttct
     3121 caaatgattg ggtagggaga tagggatctt cctttaaagt ctgaataacc ttctcaaatt
     3181 gaggtttcaa attagactgt ttgattttct ttaaatcagc tttagccgag tttttaatca
     3241 tcacagaata gttattcatt ataagttatc ccaatcaatt tcatcaacat tagtaaatcc
     3301 actatcatct ttttctcgct tacgaacaac acccaaagtg cctatttgct caagtaatag
     3361 cgtttctttg attgctttcc aatcatccaa accaataagc accgcattat tttcactgcg
     3421 ctcactaatg atttcaattt ccgtatgatt ctcatttaca tctttcaaca acttataaaa
     3481 atttttccgt gctgagctag gatttaagac tgtttttgcc atatctactc cccctctaat
     3541 ttatatctcc attctaacgt acttaattaa gtacgtaaat tcgattgttt tgtttaaatt
     3601 ggagtaccaa aacagcattg taaagtgtta tccgtttcct ttatttttat taacaagttt
     3661 tatcacaggc ttcatatatt tgattataac ttcatcttct acgtagatat ctgttatcat
     3721 tacgacatct gtactatttc gattgagtac tttcacttga tctccagcat tgattttccc
     3781 tctaaatctt ttaggaatga accaactata catatgatta gcatcaccaa aatgtgcttt
     3841 tatcgcattc tcaggatata acttattcaa tagttcattc tttttaattt tcaaaacttc
     3901 aaatctatac ttatcatcat ctgctaatct aaataaatct tctaaacgca tgttttctaa
     3961 ctgtaagttt tgaaaatctt ccggtataaa ttttcggttc tgttgcgaag tttgaaaatc
     4021 aaacgcgccc tctctgaact ccaaatatct taggctgtta ttcccattaa taccttgatt
     4081 tcagtagaca ccgaaaagct gaagagcgtt ccatttcttc ggttcttttt atatattcct
     4141 cgaatggtct ccatgccctt aatcgtggaa gaggctgtac ggagactttg ataaaattta
     4201 tt
//
LOCUS       DACEEZ010000146         4185 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20313_2017
            SAMN12639966-rid9879223.denovo.152, whole genome shotgun sequence.
ACCESSION   DACEEZ010000146 DACEEZ010000000
VERSION     DACEEZ010000146.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639966
            Sequence Read Archive: SRR10023158
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4185)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4185)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741683.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741683.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:44:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,016
            CDSs (total)                      :: 2,962
            Genes (coding)                    :: 2,889
            CDSs (with protein)               :: 2,889
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 36 of 73
            Pseudo Genes (incomplete)         :: 29 of 73
            Pseudo Genes (internal stop)      :: 18 of 73
            Pseudo Genes (multiple problems)  :: 8 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4185
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639966-rid9879223.denovo.152"
                     /isolate="E20313_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(3..1223)
                     /locus_tag="IWO89_002772"
     CDS             complement(3..1223)
                     /locus_tag="IWO89_002772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015509263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HAQ0457461.1"
                     /translation="MKLIGIVGSAAELSYNRKLLTFIQKQYHELFNLEIIEIKDLPLF
                     NQDDDQTNSPAIQQLNRKILMADGVIIATPEHNHTVPAGLKSALEWLSFKIHPLENKP
                     VMVVGASYYDQGTSRAQLHLRQILDAPGVNALVMPGNEFLLGKAKEAFDQEDRLIDPR
                     TVDFLGSTLASFVKFIKVVSTLAGPKKLPPEDLHATGTIATTIPDVDMSDDHWLEKAS
                     EAVGAVSGNTYVKLNRGLLTVDQINYFLDSMPMELTFADNNNQFLYYNLRQEAKEMLA
                     ARVPGQVGNPLANCHPEKSYKNVEWVIQQLRSGATDAVRVHVPKQVPEKYIVHNYQAM
                     HDENGEYAGINEYILDFKPIIEWYLQQTGQQLVGNEDAVSSASVHGGKTDAVTSASTH
                     DETAVDAVSSASVK"
     gene            complement(1243..1848)
                     /locus_tag="IWO89_002773"
     CDS             complement(1243..1848)
                     /locus_tag="IWO89_002773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005234653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HAQ0457462.1"
                     /translation="MKFIGLVGTNSTRSTNRELLQFMKNYFVNEAEIEIVEIKELPLF
                     NKPENTELPNVVKEIAGKIEQADGVIISTPEYDHAVPASLINALNWLSYGIFPFVDKP
                     VMITGASYGTLGSSRAQAHLRQILDSPELKARIMPSSEFLLGHSLQAFDEQGRLIDEE
                     QVAKLRELFKDFLLFVTITSQLQHAHQANKREAESFSWETI"
     gene            complement(1897..2829)
                     /locus_tag="IWO89_002774"
     CDS             complement(1897..2829)
                     /locus_tag="IWO89_002774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase"
                     /protein_id="HAQ0457463.1"
                     /translation="MNQVIEETKIWQMMGTTITLQVGHEEPSRLLAELGEWLHVYEHR
                     FSAHDATSELMAINQAAGRQAVIVHPELFELIKLGKAHSCARNSYLNIAIGPVVQKWH
                     IGFEDALVPTKAEITELLLLTDPHQIQLDEKMKTVFLTKPGMMIDLGALAKGYIADKL
                     ITHCRERNAAFALVNLGGNIVVFGESPRRSDGAWRIGLRNPKGSREESLAVVKLNNQS
                     LVTSGIYERYLQNGSSVYHHIIDPKTGFPVKNDVASLTILSDSSVDGEIWTTRLFGAS
                     SLEVLKALEEQPGIEGAVVTKENQFLYTKGFEKI"
     gene            complement(3250..4110)
                     /locus_tag="IWO89_002775"
     CDS             complement(3250..4110)
                     /locus_tag="IWO89_002775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAQ0457464.1"
                     /translation="MNIEKFITARKAANLSQKEVSEGICTQATLSKFENNGQVPSLKI
                     LIKLCNRINLPLVELFSSMNVINTQLDEQLEKAEFSLITSEYEQLTQILAAIDPKVID
                     ESELIARYYYLKGFEMIFTNAPITDVLFHFDQLLMAETANTLFRLLAYTGIGMVYARE
                     KELEKAEVYFNRVFEKIYTYPIRTTEDIWRVLNIVFHCGVFFAQDGDLETSDALLNYA
                     IDICSDNHLTYYLARAAAQLAKNAQAKNKSKAEVLELIQDALAFAKINKNKKLIDELN
                     HMKKMILLIN"
BASE COUNT         1187 a          847 c          688 g         1463 t
ORIGIN      
        1 ttttatttta cagaagcact cgaaactgca tctactgcag tttcatcgtg tgtgctggcg
       61 ctggttactg catctgtttt tccaccatga acagatgcac ttgaaaccgc atcttcattt
      121 ccaacaagtt gttgacccgt ttgttgtaaa taccactcaa tgattggttt gaaatcaaga
      181 atgtattcat tgattccagc atactctcca ttttcatcat gcattgcttg gtaattatgc
      241 acaatatatt tttctggcac ttgttttggt acatgaacac gtaccgcatc tgttgctccg
      301 cttcgcagtt gttgaattac ccactctaca tttttatacg atttttctgg gtgacagttg
      361 gctaatggat tccctacctg accgggcaca cgagcagcta acatctcttt cgcttcttgg
      421 cgcaaattat aatataaaaa ttggttatta ttatctgcaa aagttaattc catcggcatc
      481 gaatctaaaa agtaattgat ttgatccact gtcaataacc cacgatttaa tttgacgtaa
      541 gtatttccag aaacggcacc gactgcttca gaagcttttt ccaaccaatg atcatctgac
      601 atatcaacat cgggtattgt cgtagcaatt gttcctgtcg catgcaaatc ttctggtgga
      661 agctttttag gccctgctaa ggtagacacg actttaatga attttacaaa acttgctaat
      721 gtactgccta aaaagtcaac agttcgtgga tcaatcaagc gatcttcttg gtcaaatgct
      781 tcttttgctt ttcccaataa aaattcattt ccgggcatca ctaatgcatt cacgcctggt
      841 gcgtccaaaa tttggcgcaa gtgtaattgc gcgcgtgaag ttccttgatc gtagtaagaa
      901 gcacccacaa ccattacagg tttattttca agtggatgga ttttaaatga tagccattca
      961 agtgcacttt tcaatcctgc tggaaccgta tgattgtgtt ctggcgtagc aataattacc
     1021 ccatcagcca ttaagatttt gcgatttagc tgttgaatag caggactatt tgtttgatca
     1081 tcatcttgat tgaacagagg taaatctttg atttcaatga tttctaaatt aaatagttca
     1141 tgatattgtt tttggataaa tgttaataat ttacggttat atgaaagttc agctgctgat
     1201 ccgacgatac ctattaattt catctttgtt tgctcccctc gattaaatcg tttcccatga
     1261 aaagctttcg gcttcacgtt tatttgcttg gtgcgcatgt tgcaattgac tagtgattgt
     1321 gacaaagagt aagaaatctt tgaatagttc acgtaatttt gcgacttgtt cttcatcaat
     1381 gagtcgtcct tgctcatcaa aggcttggag tgaatgaccc aataaaaatt ctgagcttgg
     1441 cataattcgt gcctttaact ctggtgaatc caatatttgg cgcaaatgtg cttgtgcgcg
     1501 tgaagaacct aacgtgccat aagacgctcc ggtaatcatc actggtttat ccacaaacgg
     1561 aaaaatacca tatgaaagcc aatttaatgc gttaatcaat gaagcaggaa ccgcatggtc
     1621 atactctggt gtgctgatga ttacgccatc tgcttgttca atttttccag caatttcttt
     1681 aacaacattt ggtagttctg tgttttcagg tttgttaaat agtggcagct ccttgatttc
     1741 gacaatttcg atttctgcct catttacaaa ataatttttc ataaactgta ataattcacg
     1801 gtttgttgac cgcgttgaat ttgttcctac taatccaata aatttcatct tcattctctt
     1861 tcctttctaa catcgatttc tgactgttcg cacgtactaa attttttcaa atccttttgt
     1921 atacaagaat tgattctctt ttgttacaac tgctccctcg atacccggct gttcttcaag
     1981 cgctttcaac acttctaaag aagatgctcc aaataaacgt gtagtccaga tttctccatc
     2041 tacagaagaa tcagacaaaa tcgtcaggct tgctacgtca tttttaactg gaaagccagt
     2101 tttcggatca atgatgtgat gatacactga cgaaccattt tgtagataac gttcgtagat
     2161 tccagaggta actaaagatt ggttgtttag tttaaccacc gcaagcgact cttcacgtga
     2221 acctttagga tttcgcaaac caatacgcca agcaccatcc gaacgtcttg gtgattcacc
     2281 aaaaacaacg atatttcctc ctaagttaac taaagcaaac gctgcatttc gctctcggca
     2341 atgtgtaatt aatttatctg caatgtaacc ttttgctaac gcccccaagt caatcatcat
     2401 tcctggtttg gttaaaaaaa cggttttcat tttttcatct aattgaattt ggtgtggatc
     2461 cgttagcaga agtaactcag ttatctctgc ttttgttgga accaatgcat cttcaaatcc
     2521 aatgtgccat ttttgtacaa ccggaccaat agcaatattc agataactat ttcttgcaca
     2581 actatgtgct tttcctaatt tgatgagctc aaaaagttca ggatgtacaa tcacagcttg
     2641 tcgtccagca gcttgattga tggccattaa ttcagaagtt gcatcatgtg cgctgaagcg
     2701 atgttcataa acgtgcaacc attcgcctaa ctctgctagt aatcgactag gctcttcatg
     2761 cccaacttgt aatgtaattg ttgtccccat catttgccag attttcgttt cttcgatcac
     2821 ctgattcatt ttgtaccttc tttacttgaa ctacggatta catgagtaat tagtttttat
     2881 ttttgttact gttttcacaa ccagatgata cgcctgctag atttatttgt caaccgattt
     2941 tatatatttt tttatataat aaggtaattt taatataaaa ttttatattg taaaaaaaca
     3001 aataagggaa acaaacaaca catttttgtg tgttgcttat ttccctctaa ctatcgtttt
     3061 ctatttgatg gttaacctac ttaaaataaa tactgcaacg acaaacggag atcaagcatg
     3121 ctttgtaacg tattttacga taattacgac tttattttat tactctatct tgctgccata
     3181 cccgtctatc cctaatcatc tttggtgaat tttttcaaag ttagcataac aaaaataagt
     3241 attttttcct tagttaatca atagtatcat ttttttcata tgatttaatt catcaattaa
     3301 ttttttgttt ttattaattt tagcaaaagc tagtgcatct tgaatcaatt cgagcacttc
     3361 tgccttcgac ttatttttag cctgtgcgtt cttcgctaat tgggcagcag ctcgtgctaa
     3421 gtaataagtc aaatggttat ccgaacaaat atcgattgcg taatttagta gtgcatcact
     3481 tgtttccaaa tcaccgtctt gcgcaaaaaa aacaccacaa tgaaagacga tatttaacac
     3541 ccgccagata tcttctgttg tacgaatcgg atacgtataa attttttcaa acactcgatt
     3601 gaaataaact tctgcttttt ccaactcttt ttctctagca tagaccattc caatacctgt
     3661 atatgccaat aaacgaaaca acgtgttggc tgtttctgcc atcaacaact gatcaaaatg
     3721 gaacaacaca tccgtaattg gtgcattcgt aaaaatcatt tcaaatcctt ttagatagta
     3781 gtaacgtgca atcagctcag actcgtcaat taccttggga tcaatggctg ctaaaatctg
     3841 tgtcaattgt tcatattcac tcgtaatcag tgaaaactct gctttttcta gctgttcatc
     3901 aagttgtgta ttgatcacgt tcatcgaaga aaaaagttca actaacggaa gattgattcg
     3961 attacataat ttgattaaaa tttttaacga agggacttga ccattgttct caaatttact
     4021 taatgttgct tgtgtacaaa ttccttcaga cacttctttt tgagataagt tggcagcctt
     4081 tcttgccgta ataaattttt caatgttcat tattttctct ccaatcagac ctcacacgaa
     4141 aatcatttct gtctcgattt tagcatgttt tctttgggaa tgctt
//
LOCUS       DACEEZ010000147         4152 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20313_2017
            SAMN12639966-rid9879223.denovo.153, whole genome shotgun sequence.
ACCESSION   DACEEZ010000147 DACEEZ010000000
VERSION     DACEEZ010000147.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639966
            Sequence Read Archive: SRR10023158
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4152)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4152)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741683.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741683.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:44:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,016
            CDSs (total)                      :: 2,962
            Genes (coding)                    :: 2,889
            CDSs (with protein)               :: 2,889
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 36 of 73
            Pseudo Genes (incomplete)         :: 29 of 73
            Pseudo Genes (internal stop)      :: 18 of 73
            Pseudo Genes (multiple problems)  :: 8 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4152
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639966-rid9879223.denovo.153"
                     /isolate="E20313_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            <1..106
                     /locus_tag="IWO89_002776"
     CDS             <1..106
                     /locus_tag="IWO89_002776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HAQ0457465.1"
                     /translation="ASKRNNIPRKSLDYRTPLEVFLSYVSIDDLSNLI"
     gene            970..1182
                     /locus_tag="IWO89_002777"
     CDS             970..1182
                     /locus_tag="IWO89_002777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0457466.1"
                     /translation="MVKAEVGRSYICKPIGVSHELIGCIERTYINTAVVYVESYDQRD
                     YQLINESKYRMLVKFCDISAPAELVS"
     gene            complement(1460..3259)
                     /locus_tag="IWO89_002778"
     CDS             complement(1460..3259)
                     /locus_tag="IWO89_002778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HAQ0457467.1"
                     /translation="MNYLKRSLGNAWDFLKGTQAYFRDVLLMHGFILFICLPLLSSTT
                     RFILHRGAIDYLSYDNIPSIFSQHPGVLLSLIVVLMTILLLVYFEFTFLLMSVFFIKK
                     KEPISLKQLLHLTILQLKKVRPITFLFFLAYFLLILPISGLSFNSDLLSKIKIPAFIM
                     DFIFANRWIIVSSFLLVYIFLGYIGIRLIFALPEMILRDRPFRAAIRESWSLTKSRLL
                     AITGQFIVIGGTILLLSSLGYIVVILAQSMVEQFFPDYALISAVFAMTLLQGILLFNI
                     VMSTVGIFYIIVDFMDDEGFLPEIPKWFIPQAPNLRFSALKNTGLTLFAVFFGIGVCL
                     YNMDYLTSAVQTKPVTVSHRGVSTQNGAQNTLEALEKTSRDYHPDYVEMDVQETKDGH
                     FVVMHDANLRHLTGVNGTPQDLTLKELTNLTVTENGVQAPVCSFDSYLEKAHKLNQKL
                     LIEIKTSKKDSDDLVERFVHKYRKVILENHHILQSLTYQTVSDLKEAEPNFYVGYILP
                     FNIVGPPVTPADFLTMEYSTINRNFINSAHQDGKEVYVWTINDSDAMSRMMFYGVDGI
                     ITDEMGLLNESVKQTDEEITYSDKLLNFVLGVG"
     gene            complement(3383..3979)
                     /locus_tag="IWO89_002779"
     CDS             complement(3383..3979)
                     /locus_tag="IWO89_002779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HAQ0457468.1"
                     /translation="MNIAASRKLINFISIIGLGLSIALTIYFINLGVFKDLNALRGLV
                     GDSIILGPIIFVFIQILQVVIPIIPGGISTAAGVLIFGPYAGFIYNYIGICIGSIIIF
                     LLGRRYGKPFILSMISDKTYNKYIGWLDNQNRFEKLFALAIFLPVAPDDALCLMAGLT
                     NMSVKRYTLIILIAKPLSIFLYSMALIYGGQYLSGLLG"
BASE COUNT         1490 a          728 c          707 g         1227 t
ORIGIN      
        1 cgcatctaaa cgaaataata ttcctagaaa atcactggat tatcgaacac ctttggaagt
       61 atttttgagt tacgtaagta ttgatgatct gtctaactta atttgacaat taagattata
      121 ttaaatatta tacagataat aacggaatat tatcatcgaa acagctaaca aaattgtcat
      181 aaaaaacgag atggttgata ttagaacttg tgtttttgtt taggtctaga gataaagaga
      241 gacaatttcc aaatatatgt aatgaataca aaaagacgtt tgtcgcggat taccgttaca
      301 aacgtctttt attgattttc cgagatctca tcagtcgcca tttggaatgg ttcactcctt
      361 atctagattc gtttaatgct cggactgctt gttcgattgt aataccaaac gcaacgttat
      421 tttcgttttg tgcatcaatc gccataaaac ggttagaagt tgtatcaaaa gctacgcggt
      481 attgcatatt tgtttttgta aacgtcataa aattcatctc ctttgtaatg taatttagtt
      541 cttgaaagtt tcctttaaac tttcgaactt ataccaagtt tagcatcgta cgttttttga
      601 tgcaattaaa aagcatttat cattttcctt atttattttt tcttaaatgg tttagctatt
      661 ttcttttaat ccgataagta gcccaatcat caaggttaca aatactattg cttgaatata
      721 aatcatatgc tcacctcttt ataaataact tagcaatata aagaaaaaaa cgcacgaaca
      781 acataggaat ataaacaaac gtttttgtca caacgtctaa gatcgtgtta aaaaatagtt
      841 atctaaaaag cagaaagata ctcaaaataa aaaataaaaa aatggataaa aacgttttca
      901 aatacaaggt aatttgttat tataaaataa agaaaaaaca aaataaaaat aaagaaaaga
      961 gggaatgaaa tggtaaaagc agaagtagga agatcttata tatgcaaacc gatcggagta
     1021 tctcatgaac taatcggctg tattgaacgt acatatatca acacggctgt cgtgtatgtt
     1081 gaaagttatg accaacgaga ctatcaactt atcaatgaaa gcaagtatcg catgctcgta
     1141 aaattctgtg atattagcgc acctgctgaa ttggtttctt aaacagtatt tttgagaatt
     1201 cgcaagaact cccttataat ataaataaag acagaaggga gcgagaagat atgccaaaaa
     1261 gaaaaagttc agggaaaatg ggaattattc ttgcagtgat ctctgcaatt cctgctattt
     1321 tagctattgt tcgtgcgatg aaggaatcaa gagaaagtta agcacacatt atgagcgtca
     1381 aacaaaaaac gcatatacct gcggtggcag gttatgcgtt ttttgttgta taaagacttt
     1441 tttcattaac gatttttgat tagccgactc ccaaaacaaa atttagcagt ttatctgaat
     1501 aagtgatttc ttcatctgtt tgtttcacac tttcatttag tagtcccatc tcatcggtta
     1561 tgataccatc cacaccataa aacatcatgc gactcatagc atctgaatcg ttgattgtcc
     1621 atacatatac ttcttttccg tcttggtggg cagaattaat gaagttacgg ttgatcgtac
     1681 tatattccat tgtcagaaaa tcagcaggcg tcacaggggg cccaacaata ttgaaaggta
     1741 agatgtagcc cacataaaag tttggttctg cttcttttaa atctgaaacg gtttggtacg
     1801 tcaaactttg caaaatatga tggttctcta agataacttt gcggtattta tggacaaatc
     1861 gttcgacaag gtcatccgaa tcttttttag aggtcttgat ctcaataagt aatttttggt
     1921 ttagcttatg tgctttttcc aaataagaat caaaagaaca aacaggggct tgcactccgt
     1981 tttcagtaac tgtaagattt gtcaattctt tcaatgtaag atcctgtggt gttccattta
     2041 cccctgttaa gtgacgaaga tttgcatcat gcatcactac gaaatgacca tcttttgttt
     2101 cctgaacatc catctctaca taatcaggat gatagtctct gcttgttttt tcaagtgctt
     2161 ctaaagtatt ttgcgcccca ttttgggtac tcacacctct gtgagagaca gtcactggtt
     2221 ttgtctgaac agctgaagtc agataatcca tattgtaaag acagacgcca ataccgaaaa
     2281 aaactgcaaa aagagtcaaa ccagtgtttt tcagcgcaga gaaacgcaag ttgggggctt
     2341 gtgggataaa ccacttaggg atctcaggca agaagccttc gtcatccatg aaatcaacaa
     2401 tgatataaaa aatccctacg gtagacatca cgatattgaa taataaaatc ccttgtaaaa
     2461 gcgtcatagc aaacacagcg ctgatcaatg catagtctgg gaaaaattgt tcaaccatac
     2521 tttgtgccaa aataacaacg atatatccta aagaagaaag gagtaaaatc gtaccgccaa
     2581 tcacaataaa ttggcccgtg atggccaaaa gacgcgattt ggtcaatgac cagctttccc
     2641 gaatggctgc tcgaaaagga cgatctcgta gaatcatttc cggtaaagca aaaatcagtc
     2701 gaatcccaat atagccaagg aaaatataaa cgagtaaaaa ggaactgacg atgatccaac
     2761 gattagcaaa aataaaatcc ataatgaaag cggggatttt tatttttgac agtagatcag
     2821 aattaaaact taatccgctg atcggtagaa tcaataaaaa ataagcaagg aaaaacaaaa
     2881 aagtaatggg tctcactttc ttcagctgaa gtatcgtcaa gtgtaagagt tgcttcaaag
     2941 agattggttc tttcttttta ataaaaaaaa cactcatcaa aagaaaagta aattcgaaat
     3001 aaactaacag taaaatcgtc atcaaaacta cgattaacga gagtagaacc ccaggatgtt
     3061 gagaaaaaat tgaaggaata ttgtcataag acaaatagtc aattgcccct cggtgtaaaa
     3121 taaaacgggt cgtactgctc aataagggta aacaaataaa taaaataaag ccatgcatca
     3181 gcaagacatc acggaaataa gcttgtgttc cttttaaaaa gtcccaagca ttacctaagc
     3241 tacgtttcaa ataattcatc ctatcacctg ctcttcacat tgtagcgcaa atctaatgaa
     3301 aaaggaaagg gtgcagtgtg ttttgcagac cctttcccag atttaacatt gtcaatcaat
     3361 gactaatttt acaaaataaa tattatccaa gaagaccact tagatattga cctccataaa
     3421 tcaaggccat actatataag aaaatcgaca gtggtttagc aatcaaaatg attagtgtat
     3481 atcgtttcac tgacatgttt gtcaatccag ccatcaaaca taacgcatcg tcaggagcaa
     3541 ctggaagaaa aatagctaga gcaaacagct tctcaaaacg gttttgatta tccagccagc
     3601 caatatattt gttatatgtc ttatcgctaa tcatggaaag tatgaatggc tttccataac
     3661 gacgccctag cagaaaaatg ataatcgaac caatgcagat tccaatatag ttatagataa
     3721 atccagcata agggccaaat atcaggacac cagctgcagt actgatccct ccaggaatga
     3781 ttggaataac tacttgcagg atttggatga agacaaaaat aatcggtcct aaaatgatgg
     3841 aatcacctac taatcctctt aacgcattca aatctttgaa aacacctaaa ttaataaaat
     3901 aaatcgtcag cgcgattgat aggcctaatc cgataatgga tatgaaattt atcagttttc
     3961 gagaggcagc aatattcatt attcatcaac tccttagagg ttctttgtta actatacttt
     4021 actagaaaag caactaactg cgccattttt tgatatgaca gaaaacaaac aattttgtaa
     4081 gttcatcata agtataaaag attgatctca aaaaaacatc caactaaaga cttatttaat
     4141 aacttgtttt tt
//
LOCUS       DACEEZ010000148         4125 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20313_2017
            SAMN12639966-rid9879223.denovo.154, whole genome shotgun sequence.
ACCESSION   DACEEZ010000148 DACEEZ010000000
VERSION     DACEEZ010000148.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639966
            Sequence Read Archive: SRR10023158
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4125)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4125)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741683.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741683.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:44:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,016
            CDSs (total)                      :: 2,962
            Genes (coding)                    :: 2,889
            CDSs (with protein)               :: 2,889
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 36 of 73
            Pseudo Genes (incomplete)         :: 29 of 73
            Pseudo Genes (internal stop)      :: 18 of 73
            Pseudo Genes (multiple problems)  :: 8 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4125
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639966-rid9879223.denovo.154"
                     /isolate="E20313_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(150..425)
                     /locus_tag="IWO89_002780"
     CDS             complement(150..425)
                     /locus_tag="IWO89_002780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001196543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system protein PrgO"
                     /protein_id="HAQ0457469.1"
                     /translation="MALLPTNKDKKKIGAEDISIKDDINVELGERKYTAKERKPVQVD
                     PPVLKTIRDLSYAKDIPMYEIVKLAMDAYVDTLNDEERIIYNRRSKD"
     gene            complement(397..1350)
                     /locus_tag="IWO89_002781"
     CDS             complement(397..1350)
                     /locus_tag="IWO89_002781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HAQ0457470.1"
                     /translation="MENLRTAFKTSDKLGKVVMLTGRKGGIGKTTDNDLLAIVSSQLF
                     EKDVLLIDYDQQRNTTSNIGSTYQITSFDRSMSAAIKKGDWVSGITQVSPHLYIMAGS
                     PGSEELNEYLSEKYPDRRKRSLAFIKPLEELRKNFDYIFIDCPPSTDNVVRAFLTAAD
                     YIIAMQELKRYAMEGTEDFINKVLVPIVTNFEESHLQIIGILPVLFSVRRSSQHANYQ
                     KTIDKYGESNIFKSIVKGSDRLEMYGENGIQLNDYVDRRWWAIFADIFTELEERIEYY
                     EQHGDIEGFDYTPKYADSMANHILPLGKEIKLNGIITNEQR"
     gene            1962..3455
                     /locus_tag="IWO89_002782"
     CDS             1962..3455
                     /locus_tag="IWO89_002782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000947690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein RepR"
                     /protein_id="HAQ0457471.1"
                     /translation="MLSEIYSAILKNGLRTYKYRNSTMKPAGHIDSSRSGAIFAYRSK
                     QLMNVGRGMVITSEEAILENEKKLTHWTPNTYRFGTYADDYRTVVKGHSEKNLSQINT
                     FVVDIDSKENHQGEIILACLDQVGYMPTLILESDHGYQVYFVLKTPGYVTKKSNFKVI
                     DVAKKISKTIRLQLAEKLSGVDLGCNHFGIARFPQENNIVFKEMNYQYSFADWIQWSM
                     KLESDQKTKEDRERKLIVFPEKKEIRQVDEPWFDMLLHKANIVGGEGRLGRNNVIFTL
                     SLAYYSSGYALDTCEYNMFTFNERLQEPLSEAELVKIVRSAYSGNYQAASREFIIELC
                     QQWVSPTLKNNDLFIQRRGWWKFKKPRDQRKYSHKNEWKEDLLAYLSEKSLASEPYLN
                     LSKKELASQLTMPKRTLDKILNELREENKVFYKVTRGRNGGMIIASVKTLFARVIQLS
                     KEKRLDYSTRITEVFGLNESLVEETFRQLSTPLNPLKEVNLFEVDTG"
     gene            3590..3886
                     /locus_tag="IWO89_002783"
     CDS             3590..3886
                     /locus_tag="IWO89_002783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000053907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system protein PrgN"
                     /protein_id="HAQ0457472.1"
                     /translation="MSLKNYVYSHPVNVFIIGRLGLSVEVFCELYGFKQGTLSSWVTR
                     EKTVASLPIEFLHGLSLASGETMDQVYDCLCVLEQEYIEYKIANELRKRKKYIQ"
     gene            3996..>4125
                     /locus_tag="IWO89_002784"
     CDS             3996..>4125
                     /locus_tag="IWO89_002784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain containing protein"
                     /protein_id="HAQ0457473.1"
                     /translation="MTYTHLTPNELVMIEAYFHQETPVAIVAKQLKRGRQTIYNVYN"
BASE COUNT         1385 a          681 c          740 g         1319 t
ORIGIN      
        1 ttagtatagt aaaaactaga aacaataaaa tttgtgaatg ttgtataccc ccaaacaata
       61 cacatatttt gaaggattag gattaataaa gctaaaaata tagtaagtat actaaatata
      121 tttactatat ctagtatact tactatattt taatcttttg atcgtctatt atatataatc
      181 cgttcctcgt cgtttaaagt atcaacatag gcgtccattg caagtttgac tatttcatac
      241 ataggaatat cttttgcata actaagatcc cgaatggttt ttagtacggg aggatctact
      301 tgaactggtt ttctttcttt cgcagtgtac tttcgttccc ctaactcgac attaatatca
      361 tctttaattg aaatatcttc agcaccaatt ttcttcttat ctttgttcgt tggtaataat
      421 gccatttagt ttaatctcct ttccaagagg taggatatga ttggccattg agtcagcata
      481 tttaggtgta taatcaaatc cttcaatatc accatgttgt tcatagtatt caattctttc
      541 ttctaattct gtaaagatgt ctgcaaaaat agcccaccat cttcgatcta cataatcatt
      601 taattggata ccattctcac catacatttc taaccgatcc gagcctttaa caatgctttt
      661 aaaaatgttt gattcaccat acttatcaat tgttttttga tagtttgcgt gttgagagga
      721 acgtcgcact gagaaaagaa caggcaaaat tccaatgatt tgaagatgag actcttcaaa
      781 attcgtgaca atcggaacta aaactttatt aataaaatct tctgttcctt ccattgcgta
      841 ccgttttaat tcttgcattg caataatata atctgcagca gttaagaatg cacgaaccac
      901 attatctgtt gatggtgggc agtcgataaa gatataatcg aaattttttc tcaattcttc
      961 taaaggttta atgaaggcca atgatctctt acgacgatca ggatattttt cggacaagta
     1021 ttcattaagt tcttcagatc caggagagcc agccataata tataaatgtg gacttacttg
     1081 agtaatacct gaaacccagt cccctttttt gattgctgca gacatggatc gatcaaagct
     1141 agtaatttga tacgttgatc caatgttgga agtagtattt ctttgttggt cataatcaat
     1201 tagcaacacg tctttttcaa aaagttgact agaaacaatc gctaataaat cattgtctgt
     1261 agtcttacca atgccgcctt ttctacctgt aagcattaca acttttccta gtttatctga
     1321 tgttttaaat gcagtccgta agttttccat agttccctcc aatttcctat actaaatata
     1381 tttagtatac ttcatatact taatatattc gatatatcta atatactcca aattgataag
     1441 aattcagact ttataaggaa tatagtggat atactaaata tatttgctat attcagtata
     1501 tcagatatat tcaaaatgtc aaaatatatt tgtgcgtttt gcgttgacaa tttagagtga
     1561 aaagaggtaa acttaattct atagaaacca cagataattc actatttaca aagtagcaaa
     1621 agcagtggta ctctttaaac agaaaaatta cataaaaaaa agagacggaa ataattgatt
     1681 ttagcagaat caattatttc tgaatcacat ggttaggaca tgtgttcacg ctcgtctcaa
     1741 gaatatgtaa gtagctacca actacttata ttaaaagttg atcggatacc ttttccgatt
     1801 gcctttattc taccaaaaaa aggcgagcaa ctcaacagaa gaaagtttat ttagtgtact
     1861 taaattaaat attcttaaac actgaagaga gaacacatac tatggctgag attaaagcta
     1921 aactgtgttc tctttttgtt tgagaaagga cgttacaatc gatgttgagc gaaatttatt
     1981 cagctattct taaaaacggc ttacgtacat acaaatatcg aaacagcacc atgaaacctg
     2041 caggtcatat tgattccagt aggagtggtg ctatctttgc gtaccgtagc aagcaattaa
     2101 tgaatgttgg gcgtggtatg gttattactt cggaagaagc tattttagag aacgaaaaga
     2161 aactaacgca ttggacacct aatacctatc gctttggtac ttatgcggat gattatcgaa
     2221 cagtggttaa aggtcattct gagaaaaact taagtcaaat caacacattt gtagttgata
     2281 tcgattcaaa agagaaccat caaggcgaaa ttattttagc ctgtttagat caagtgggct
     2341 acatgccaac gctaatttta gaatcagatc atggttatca agtttatttt gtactaaaga
     2401 caccaggcta tgtcacaaag aaaagcaatt tcaaggtgat cgatgtagca aaaaaaattt
     2461 caaaaacaat tcgtctacag ttggctgaaa aattgtccgg agtggatcta ggatgtaacc
     2521 acttcggaat tgctcgattc ccacaagaaa ataatattgt ttttaaagaa atgaactatc
     2581 agtattcttt tgctgactgg attcaatggt cgatgaagct tgaatcagat caaaaaacaa
     2641 aagaagatcg agaacgaaaa ctaattgtct ttcctgagaa aaaagaaatt cgtcaagtag
     2701 atgaaccgtg gttcgatatg ttacttcata aagcaaacat cgtcggcgga gaaggacgtc
     2761 ttggtcgaaa taatgtgatt ttcacgcttt ctttagctta ttactcttca ggttatgcat
     2821 tggatacttg cgaatacaat atgttcacat tcaacgaacg gttacaggaa ccgctatctg
     2881 aagcagaatt agtaaagatt gtacgtagtg cttattctgg taattatcaa gcagcaagca
     2941 gagaattcat tattgaattg tgccagcaat gggtttcacc aaccttaaaa aataatgatc
     3001 tgttcattca acgacgtggc tggtggaaat ttaaaaagcc acgagatcaa agaaaatata
     3061 gtcataaaaa tgaatggaaa gaagatctat tggcttatct aagcgaaaaa agtcttgcta
     3121 gtgagcctta tttgaacctg tcaaaaaaag aattggcttc acaactaacg atgcccaagc
     3181 gaacacttga taagatctta aatgagttac gagaggagaa taaagtcttc tacaaggtaa
     3241 cacgaggaag aaatggcgga atgatcattg cttcagtcaa aacgttgttc gcgagggtta
     3301 ttcaactaag taaagaaaag agactagatt attcaacaag gatcacagag gtatttggac
     3361 tcaacgagag cttagtagaa gagacattta gacaattatc aacccccttg aaccctctta
     3421 aagaagtgaa tctttttgaa gtagatacag gttgaaaaat aaaaggtgca aattgcccta
     3481 tacaatatat ctatgatact tctgtaggtg ttgctccctt tgcctagcta cgtgtaggtg
     3541 gtatacttat aactgcgtat gcagtcttat gtatcatgga ggaagttgta tgtcgttaaa
     3601 aaattatgtc tattcacatc ctgttaatgt gtttatcatt ggtcgtttgg gattatctgt
     3661 ggaagtgttc tgtgagcttt atggttttaa gcaggggact ttgtcttctt gggttaccag
     3721 ggaaaaaaca gttgcttctt tgccgattga gtttcttcat gggttgtcct tagcttctgg
     3781 ggagactatg gatcaggtat atgattgttt gtgtgtgtta gagcaggagt atattgagta
     3841 taaaatagct aacgagttaa gaaaaaggaa gaagtatatc cagtagtagt ttttatttac
     3901 gccgtttgta taattaagcg agacaaataa aaaagccatt tatgttacac tcaaattata
     3961 atacccgcta aagcatacaa ggagagtgaa cacaaatgac ttatacccat cttacaccaa
     4021 acgagcttgt aatgatagaa gcatattttc atcaagaaac tccggttgct atcgttgcga
     4081 agcagcttaa acgtggacgc caaacaattt acaatgtcta taact
//
LOCUS       DACEEZ010000149         4080 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20313_2017
            SAMN12639966-rid9879223.denovo.155, whole genome shotgun sequence.
ACCESSION   DACEEZ010000149 DACEEZ010000000
VERSION     DACEEZ010000149.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639966
            Sequence Read Archive: SRR10023158
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4080)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4080)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741683.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741683.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:44:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,016
            CDSs (total)                      :: 2,962
            Genes (coding)                    :: 2,889
            CDSs (with protein)               :: 2,889
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 36 of 73
            Pseudo Genes (incomplete)         :: 29 of 73
            Pseudo Genes (internal stop)      :: 18 of 73
            Pseudo Genes (multiple problems)  :: 8 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4080
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639966-rid9879223.denovo.155"
                     /isolate="E20313_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            598..1287
                     /locus_tag="IWO89_002785"
     CDS             598..1287
                     /locus_tag="IWO89_002785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007207861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAQ0457474.1"
                     /translation="MKILIADDDKEIVELLSIYVHNEGYEAVKAYDGKEALSKIRTIP
                     DIELLILDIMMPEMDGMQVVKELRKESQIPIIMLTAKTTDMDKIKGLVAGADDYVTKP
                     FNPLEVMARVKSLLRRTKMQIAPDQPDVLEVNALIINKDSHEVKTIDGKEIQLTALEF
                     GILYLLASHPNRVFSADEIFERVWKQESIVSAKTVMVHVSHLRDKIEEATGGEKVIQT
                     VWGVGYKIDAR"
     gene            1274..2482
                     /locus_tag="IWO89_002786"
     CDS             1274..2482
                     /locus_tag="IWO89_002786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HAQ0457475.1"
                     /translation="MTQDSKRATEKRRRINLTSKEISELLAEGIITIILLLLLNVSIL
                     VVISSVINSSPSLTNAIWDSKNIFAERLNTDLFWNGRNFIIPFFFLLDIGVLYWRLIR
                     RYRQMQLRHIISELHYIANGNYDHRIPFELSGDLSRVVTSINGLVDSTVAAIEDERKI
                     EKSKDELITNVSHDIRTPLTSIIGYLGLIEDGQYHSEEDLLKYTHTAYIKAKQMKSLV
                     DDLFEYTKVRQPAVPVNFSAFDMIQLIEQLAADFELEASKKNIQILVQSKVDSLIMDG
                     DTEKLVRVFNNLLTNALKYGKGATKIVIEVERIGSEVVATVKNNGAMIPQQAIDNLFD
                     RFYRVEESRSQATGGTGLGLAIAQSIVALHGGYIYAKSDKQWTSFIMHLPIKKNEKLP
                     IDTQEVIDES"
     gene            2776..4047
                     /gene="serS"
                     /locus_tag="IWO89_002787"
     CDS             2776..4047
                     /gene="serS"
                     /locus_tag="IWO89_002787"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HAQ0457476.1"
                     /translation="MLDVKMMRQNIEEVQEKLMTRGVKKETLAEFMELDENRRKLLVK
                     SEELKKYRNDVSAEIAQLKRAKEDASAKIAEMKEVGGNIKALDTEIAEVDEKLKQIAT
                     TLPNLPHDSVPVGTDEEDNVEIRRWSEPRTFAFEPKPHWEIAENLDILDFERGAKVAG
                     SRFVYYKGLGARLERAVYNFMLDQHIYEHGYTEMITPYIVNSKAMFGTGQFPKFKEDV
                     FQLENTDLTLIPTAEVPLTNYYNDEILDGADLPIYFTALSPAFRSEAGSAGRDTRGLI
                     RLHQFNKVEMVKFSDVEHSYEELEKMTADAEDILKKLHLPYRVMALSTGDMGFSAAKT
                     YDLEVWIPAQDTYREISSCSNCEDFQARRAMIRYRDNDGKIHYTHTLNGSGLAVGRTV
                     AAILENYQNEDGSVTVPEALVPYMGNLKVIK"
BASE COUNT         1322 a          705 c          812 g         1241 t
ORIGIN      
        1 ttcatcagga gtgtttcccg attcgttttt ggctgccgta gcaactttta tatcatatca
       61 gagagacaag taattgtcaa ctctttttta aaagtttttt cgatttcgtt tttcgaaatc
      121 ttccagcaac tcatttatca taacttatcg tttgttattt gtcaagaact tttttgaagt
      181 ttttttcgat agaatcaagc ttcgcaagtg gttctttttt aacgactcag ttatcttatc
      241 atgtcatttc caacatgtca acaacttatt ttttattatt taataagtta ttggtgttct
      301 tttgaacaaa taataatata tcaacatcac atgtgaaagt caataagaaa atgcttattt
      361 atcaacattt ctagcttaca tgatcattgg ttagttctgg tcctttttcg ttcgtcttta
      421 taactccgag agacgtaaaa tccttttgtt ttcagtttaa tgttcatgtt cttgtcttat
      481 tcacaccaac tagatgtctc accttctaaa attttcctta acaaagtgct gcttttatct
      541 atttctgctt ttttttgcta gaataatagt aacaatatga ctctggagga tttatttatg
      601 aaaattttga ttgctgacga tgataaagaa atcgttgaat tattaagtat ctatgttcac
      661 aatgaggggt atgaagcagt aaaggcttat gatgggaaag aagcattatc taaaatcaga
      721 acaatccctg atattgaatt acttatttta gatatcatga tgcctgagat ggatgggatg
      781 caagtagtga aagagctacg aaaagaatcc caaataccaa tcattatgct gacagcaaaa
      841 actactgata tggataagat caaaggactg gtcgctggtg cagatgatta tgtgaccaag
      901 ccatttaatc cgttagaagt aatggctaga gtaaagtctt tattacggcg tacaaaaatg
      961 cagattgccc cagatcagcc tgatgtatta gaagtaaatg cattaatcat caataaagac
     1021 tctcatgaag ttaaaacaat cgatgggaaa gaaatccagt taacagcttt ggaattcggt
     1081 atcctctact tgcttgctag tcatcctaat cgagtattta gcgcggatga aatttttgag
     1141 cgtgtttgga aacaagaaag tattgtctct gctaaaactg tcatggttca cgttagtcat
     1201 ttaagagata agattgaaga agcaactggt ggtgaaaaag tcattcaaac cgtttgggga
     1261 gtgggctata aaattgacgc aagatagtaa acgagcaact gaaaaacgcc gtcggatcaa
     1321 tttgacttca aaagaaatca gcgaattgtt agcggaagga atcatcacga ttatcctttt
     1381 gcttctatta aatgtttcta ttctggttgt tatcagttcg gtgatcaaca gttcaccttc
     1441 tttgacgaat gcgatttggg attcaaagaa tatttttgca gaacgattga acactgattt
     1501 gttctggaac ggccggaatt ttattattcc ctttttcttt ctattagata ttggtgtttt
     1561 gtattggcgc ttgattcgaa gatatcgcca aatgcaattg cggcatatca tcagcgaact
     1621 tcactacatc gcaaacggaa attatgatca tcgtatccct tttgaactga gtggtgactt
     1681 aagccgagtg gtaacaagta tcaatggatt ggtggatagt accgtagcag cgattgaaga
     1741 cgaacgtaaa atcgaaaaat caaaagatga attgatcacg aatgtcagtc atgatatccg
     1801 taccccactc acgtccatca ttggatacct aggactgata gaagatggcc agtatcactc
     1861 agaagaagat cttttaaagt atacgcatac tgcttatatc aaagcaaagc agatgaaatc
     1921 tttagtggac gacttatttg aatacactaa ggtacgccag cctgctgtcc cggtgaattt
     1981 ttctgccttt gacatgatcc agttgatcga acagttagca gctgattttg agttggaagc
     2041 ttctaaaaaa aatatccaaa tacttgttca atcaaaagta gactccctta tcatggatgg
     2101 cgataccgaa aaacttgtac gtgtcttcaa taatcttttg acgaatgcgt tgaaatacgg
     2161 aaaaggagca acgaagatcg taatcgaagt agaacgtatc ggttcagaag ttgttgcaac
     2221 agtcaaaaat aacggagcaa tgatcccaca acaggcaatc gataatctat tcgaccgttt
     2281 ttatcgtgta gaagaatctc gttcacaagc aactggagga actggacttg gtttagcaat
     2341 cgctcaaagc attgtggctt tgcacggcgg atacatttat gcaaaatcag ataagcaatg
     2401 gacttctttc atcatgcacc ttccaatcaa gaaaaatgaa aaattaccta ttgatacaca
     2461 ggaagttatt gacgaaagct gaaatattcg ctattcttat tacaattatt agaataaatg
     2521 aaaaacacag aacccagtac actttattct ttttcagaga gctgatggca ggtgtaaatc
     2581 agcaaaggat aatttggaag ttccagttcg tttacgtgcc ctttcgaaat aagaaaggtc
     2641 ggttcaacag ccgttatgtg ttcaacaagt gcagctgacg ctgaacttgg gtggtaccgc
     2701 gaatttcaga ctgattcgtc ccaagaataa tgggatggat cagtcttttt ttattttcaa
     2761 aaaggaggaa atcatatgtt agatgtaaaa atgatgcgac aaaatattga agaagtacaa
     2821 gaaaaattga tgactcgggg cgtaaaaaaa gaaacattag ctgaattcat ggaactagat
     2881 gaaaatcgtc gtaagctact agtcaaaagt gaagaattaa aaaaatatcg aaatgatgtt
     2941 tctgctgaga tcgcccaatt gaagcgtgca aaagaagatg cttctgctaa aatcgctgaa
     3001 atgaaagaag tcggtggcaa catcaaagca ttggatactg aaattgctga agtggatgaa
     3061 aaattgaaac aaattgcaac tactttgcca aaccttcccc atgattctgt gccagttgga
     3121 acagatgaag aggacaatgt agaaatccgt cgttggagcg aaccacgtac atttgctttt
     3181 gaaccaaaac cacactggga aattgcagaa aatcttgata ttttagattt tgaacgtggc
     3241 gcaaaagtag ccggtagccg ttttgtctat tacaaaggat taggtgctcg attagaacgt
     3301 gctgtctaca acttcatgct tgatcagcat atttacgaac acggctatac tgaaatgatc
     3361 actccttata tcgtcaacag taaagcgatg tttggtacgg gacaatttcc aaaattcaaa
     3421 gaagatgttt tccaattgga gaatactgat ctaacattaa tcccaacagc tgaagtacca
     3481 ttaactaact attataatga tgaaatttta gatggtgcag atttacctat ttatttcact
     3541 gcactaagcc ctgcttttcg ttctgaagcc ggaagtgctg gtcgggatac acgaggactg
     3601 attcgtcttc accaattcaa taaagtagaa atggtcaaat tttcggatgt agagcattct
     3661 tacgaagaat tggaaaaaat gacagctgat gcagaagata tcttgaaaaa attgcatttg
     3721 ccttatcgtg tgatggcctt atcaactgga gacatgggct tttctgcagc taaaacttat
     3781 gacttagaag tatggatacc tgcacaagat acttatcgtg aaatcagttc ttgttctaac
     3841 tgtgaagatt tccaagcacg ccgcgcaatg atccgttatc gcgataatga tggaaaaatc
     3901 cactatactc atactctgaa tggttcagga cttgcagttg gccgtacagt agctgccatt
     3961 ttggaaaact accaaaatga agatggttct gtcacagttc cagaagcgct cgttccttat
     4021 atggggaatc tgaaagttat taaataaaga ggctgtgaaa aagccgctta gtttcgagta
//
LOCUS       DACEEZ010000150         4055 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20313_2017
            SAMN12639966-rid9879223.denovo.156, whole genome shotgun sequence.
ACCESSION   DACEEZ010000150 DACEEZ010000000
VERSION     DACEEZ010000150.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639966
            Sequence Read Archive: SRR10023158
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4055)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4055)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741683.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741683.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:44:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,016
            CDSs (total)                      :: 2,962
            Genes (coding)                    :: 2,889
            CDSs (with protein)               :: 2,889
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 36 of 73
            Pseudo Genes (incomplete)         :: 29 of 73
            Pseudo Genes (internal stop)      :: 18 of 73
            Pseudo Genes (multiple problems)  :: 8 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4055
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639966-rid9879223.denovo.156"
                     /isolate="E20313_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(97..1563)
                     /locus_tag="IWO89_002788"
     CDS             complement(97..1563)
                     /locus_tag="IWO89_002788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAQ0457477.1"
                     /translation="MNNKQLRWFTVALIAFNMVWGMGNVVNNYAQQGVSVVTSWLLIL
                     AIYFIPYALIVGQLGSAFKDSKGGVSSWVENTTSNKRLAYYAAWTYWVVHIPYLAQKP
                     QAILIAGGWAVQGNGNIVNTMSVQMVAVISLIIFLAFLYLSTKGLSTLKVIGGLAGTA
                     MFVMSILFILLAVTAPFINPTMEFATPHMNQVKTYIPTFDFSYFTTVSMLVFAVGGAE
                     KISPYVNSTRNPAKEFPKGMIFLAAMVGICAVLGSVAMGMLFASDNIPKDLMANGAYS
                     AFQILGNYYGIGNSLMIIYALTNMIGQISALAFSIDAPLKILLADADPEFVPNWLRKR
                     NRKGTLVNGYLLTGILVGIIILLPIFGIKDMNELVKWLTNLNSVVMPMRYLWVFLAYM
                     MLNKAYKKFNSEYKFVKNPKIGFIFGAWCFAFTAFACILGMVPKVDYTADPKAWWFQL
                     FTNIITPIVLILLGMILPAIARRDKNKKITEPETVVQP"
     gene            complement(1854..3938)
                     /gene="cadA"
                     /locus_tag="IWO89_002789"
     CDS             complement(1854..3938)
                     /gene="cadA"
                     /locus_tag="IWO89_002789"
                     /EC_number="7.2.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cadmium-translocating P-type ATPase"
                     /protein_id="HAQ0457478.1"
                     /translation="MKSYRLEGLDCANCAMKIEKGVQKINGVKEATVNFTSGKLTIDA
                     EEDHLATIEQETKKVVKELEPDVKVTEIDKEKVSEHGNEKERNTLFRILFSLAGIALL
                     LLFDFNEPIRLIGYLLIYLLIGYDVVKKAVMNIVKGKIFDENFLMSVATIGAMLIGEY
                     PEAVAVMLFYQIGEYFQGLAVSHSRKSIRELMAIRPETAHVQTTEGLMTVNPEDVQIG
                     QFVLVKPGERVPLDGTIIEGESLVDTSALTGESVPKSVYVGETVLSGFINKNKPFLVQ
                     VEKSYENSTISKLLELVENASSKKAPAENFITKFARYYTPVVVGLAVLLAVLPPLVVS
                     GAAFSEWIYRALTFLVISCPCALVISVPLSFFGGIGGASKIGVLVKGSNYLELLAQTE
                     TVVFDKTGTLTKGDFSIQTIDTKIDPKKFMQYIASAEQFSTHPIAQSVLEGYAGPLLP
                     TTNIQEFAGEGILAEVDGKQVLAGNHKLMERFEISFPLSQEIGTLLYLAIDQSYSGYL
                     VIADTLKEDAVDALVQLKQAGVKNTVMLTGDSKKIADHIGKQVGVDKIYSELLPEDKV
                     QRLEEILQRNNKKTAFVGDGINDAPVLARADVGIAMGGLGSDAAIEAADVVIMNDQPS
                     KIAEAIHLAKKTLKIVKQNIVFAIGIKILVLALGAFGFASMGDAVFADVGVTVLAVLN
                     AMRSLHVKKEDE"
BASE COUNT         1225 a          865 c          642 g         1323 t
ORIGIN      
        1 cttcacaaaa atttgagaag caattttcgt aaattccttc ttatttcttg aagctgacca
       61 cttctgtcac aacctcttgt aacaagtatt tctattttaa ggctgaacca ctgtttctgg
      121 ttcagtgatt tttttgtttt tgtctcgacg agcaattgct ggcaagatca tacctaataa
      181 gatcaagacg attggtgtga tgatattcgt aaataattgg aaccaccatg ctttaggatc
      241 agcagtataa tctacttttg gcaccattcc taggatacat gcaaacgcag tgaaggcaaa
      301 gcaccaagca ccaaagataa aaccgatttt tggattttta acaaatttat attcagaatt
      361 gaatttctta taagctttat tcaacatcat atatgccaag aacacccata ggtaacgcat
      421 tggcatgaca actgagttca agttcgtcaa ccattttact aattcattca tatctttgat
      481 accaaaaatc ggcaacaaaa taatgattcc tactagtata cctgtcagaa gataaccatt
      541 caccaatgta cctttacgat tacgtttacg tagccagttt ggtacaaatt ctggatccgc
      601 atctgctaac aagattttta gtggtgcatc aattgaaaag gctaatgcgg agatttgtcc
      661 aatcatgttt gtcaatgcgt aaatgatcat caatgagtta ccgataccat agtaattacc
      721 taaaatttga aaagcactgt atgccccatt tgccatcaag tctttaggaa tattatcact
      781 agcaaacagc atacccattg cgactgagcc taatacagca caaatcccta ccattgctgc
      841 taagaagatc atccctttag gaaattcctt tgcaggattt cttgtagagt tgacataagg
      901 ggatattttt tccgcccctc caactgcaaa tactagcatc gaaacggttg tgaaataact
      961 gaaatcaaat gttggaatat atgttttcac ttgattcata tgtggtgtgg caaattccat
     1021 cgttggattg atgaacggtg ccgttacagc taaaagaata aataagatcg acatgacgaa
     1081 catcgctgtt ccggccagtc cgccaatcac tttcaaagta gaaagtcctt ttgttgataa
     1141 atataaaaag gcaagaaata taataagtga aataactgct accatttgta ctgacatcgt
     1201 atttacgata tttccatttc cttggactgc ccaaccacca gcaatcagaa ttgcttgagg
     1261 cttttgtgcc aaataaggaa tatggacgac ccagtaagtc caagcggcat agtatgccag
     1321 tcgtttatta gatgtcgtat tttcgaccca tgaactgacg cctcctttac tatccttgaa
     1381 ggctgatcct aattgtccaa cgatcaatgc gtatggaata aaataaatgg ccaaaatcaa
     1441 tagccaagag gtgacaactg atactccttg ctgagcatag ttattaacta cattccccat
     1501 gccccaaacc atgttaaatg caattaacgc aacagtaaac cagcgtaatt gtttgttgtt
     1561 cattcgttct ctcctttatg ataaaaattt gttatccaga tgtaatcata ttcaattttt
     1621 aacaaactta caagacagtg ttattttatg tgtgtattct tttatttaat acacatcgtt
     1681 ttttcatatt ttttccatca aaaaaataag ttattttctt ttatttcatt gaccttattg
     1741 aatttcaagc tattttgaat tttctgcaaa attaaaaaca ttagttattt tataatccta
     1801 tctattttct ttacattttt cattaagaaa aggatataaa aaaaagagga cgttcattcg
     1861 tcctcttttt ttacatgcaa gcttcgcatg gcattcaaaa cagctaatac tgtcacccca
     1921 acatctgcaa atactgcatc tcccattgat gcaaaaccaa aagcacctaa ggcaagtacc
     1981 aaaattttta tcccaatagc aaagacaatg ttttgtttga cgattttcaa tgttttcttt
     2041 gctaaatgga ttgcttctgc aatcttcgat ggttgatcat tcatgatgac tacatctgct
     2101 gcttcaattg cagcatcgct tcccaatcca cccattgcga ttcctacatc tgctcttgct
     2161 agcaccggtg catcgttgat tccatctccg acaaatgcgg tctttttatt gtttctttgt
     2221 aagatttcct ctaaccgctg cactttatct tcaggaagca attcgctata tatcttgtcc
     2281 acgcctactt gcttcccgat atgatcagca atttttttcg agtcgcccgt caacataact
     2341 gtgttcttca caccagcctg ttttaattga accaatgcgt ctacggcatc ttctttcaag
     2401 gtatctgcaa taacaagata accactgtaa ctttgatcga tcgctagata aagcaatgta
     2461 ccgatttctt gacttaaagg aaaagagatt tcaaatcttt ccatcaattt gtgattacct
     2521 gctaatactt gtttgccatc aacttcagct aagatccctt cacctgcaaa ttcctggata
     2581 ttagttgttg gtaaaagcgg acctgcgtag ccttctaaaa cagattgagc aatcggatgt
     2641 gtagaaaact gttctgcact cgcaatatat tgcatgaatt tctttggatc gatttttgta
     2701 tcgatcgtct ggatcgaaaa gtcacctttc gttaatgtgc cggttttatc gaataccact
     2761 gtctcggttt gcgcaagcaa ctccaagtaa tttgaacctt ttactaatac accgatttta
     2821 cttgcgcctc cgataccgcc aaagaaacta agtggcacgg aaatcactaa tgcacatgga
     2881 catgagatca ctaaaaatgt caatgctcga tagatccatt cagaaaacgc cgctccagaa
     2941 acaacaagtg gaggtaaaac agctaataaa acggccaatc ctacaacgac tggtgtgtaa
     3001 tagcgcgcga atttagtaat gaaattttca gctggtgctt ttttactgct ggcattttct
     3061 actaattcta ataatttgct gatagtagaa ttttcataag atttttctac ttgaactaaa
     3121 aatggtttat ttttattgat aaatccgctt aatactgttt caccaacata tacagattta
     3181 gggactgatt ctcctgtaag tgctgacgta tcgactaatg attctccttc gatgatcgtt
     3241 ccatctaacg gtacacgttc accaggcttc acaagtacaa actgaccgat ctgcacgtct
     3301 tcagggttca cggtcatcaa tccttccgtc gtctgcacgt gagctgtttc tggacggatc
     3361 gccatcaatt cgcgaatcga ttttcttgaa tgactgactg ccaatccttg gaaatactcg
     3421 ccgatttgat aaaatagcat cactgctacg gcttctggat attcaccgat caacatagcg
     3481 ccaatcgtag caacagacat aagaaaattc tcatcaaaga tttttccttt gacgatattc
     3541 atcactgcct tcttcactac atcatatcct atcagcagat aaatcaataa ataccctatc
     3601 aagcgaattg gttcattaaa atcaaataat agcaataaag cgatacctgc aagggaaaat
     3661 aaaatccgga acagcgtatt tctctctttt tcatttccat gttcagatac tttttcctta
     3721 tctatttctg ttactttgac atctggttct aattccttca ctactttttt tgtttcttgt
     3781 tcaatcgtag ccaaatgatc ttcctctgcg tcaatcgtta gttttccaga agtgaagttt
     3841 accgttgctt ccttgactcc attgatcttc tgaactcctt tttcgatttt cattgcacag
     3901 ttcgcacaat ctaaaccttc taatctgtaa cttttcattt taatgacctc ctcgcctcaa
     3961 catatgaaca tctcttcata actaattata aatgaatggt tattcatatg tcaaggtatc
     4021 cgaaagaaaa aatgaaaaac atctgcatcg gaaaa
//
LOCUS       DACEEZ010000151         3994 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20313_2017
            SAMN12639966-rid9879223.denovo.157, whole genome shotgun sequence.
ACCESSION   DACEEZ010000151 DACEEZ010000000
VERSION     DACEEZ010000151.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639966
            Sequence Read Archive: SRR10023158
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3994)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3994)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741683.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741683.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:44:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,016
            CDSs (total)                      :: 2,962
            Genes (coding)                    :: 2,889
            CDSs (with protein)               :: 2,889
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 36 of 73
            Pseudo Genes (incomplete)         :: 29 of 73
            Pseudo Genes (internal stop)      :: 18 of 73
            Pseudo Genes (multiple problems)  :: 8 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3994
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639966-rid9879223.denovo.157"
                     /isolate="E20313_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            140..460
                     /locus_tag="IWO89_002790"
     CDS             140..460
                     /locus_tag="IWO89_002790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0457479.1"
                     /translation="MKKRLLWCLLFLSLSLTSQPVFAEETQRSPITGTRLYKNVYVQT
                     TDLDSAIPETEERSLQPRFGYFWYCPTCDYESCWHFLHSTAAAFAFTHTQKYDGHHPV
                     IYEV"
     gene            912..1112
                     /locus_tag="IWO89_002791"
     CDS             912..1112
                     /locus_tag="IWO89_002791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HAQ0457480.1"
                     /translation="METGTVKWFNSEKGFGFITAENGNDVFVHFSAIQGDGFKTLEEG
                     QAVTFDVEEGQRGPQATNVTKA"
     gene            1259..>3994
                     /locus_tag="IWO89_002792"
     CDS             1259..>3994
                     /locus_tag="IWO89_002792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HAQ0457481.1"
                     /translation="MKPRTYAVVDIETTGTNPKEDRIIQFGCVMIESGRITSRFSIDI
                     HPGRKISKQIQHLTGITNQRVQKAPYFEDVAQTIYNLLADTIFVAHNIYFDYNFLNHE
                     LMRCGLPSLKIPGIDTVELAQIFLPTEPSFRLADLSESLGFIHENPHQADSDAEVTGQ
                     LLLLIEERMRKLPIITLEQIARLSRHTGMDTSRFIYHVIEEMKEKPEPLDPSLEIVDG
                     LALQKKEVELFTSVHYGERTYPRKKQAKEKMFGKTLMYRKEQNRLMNAVYDHFTKDES
                     KDLMIEAATGMGKTIGYLLPLSYLATPEKPAVISTVSLVLQQQIIEKDIPLLNQLLDQ
                     PIQPVIIKSYRHYIDLQRFKGTLVEPPEQKQYALYQMAVLVWLTQTKTGDLDELHLTN
                     LNHSFFADIAHRGIGFLARNQSFYEQDFVRFLQKKIRQSNFLIVNHAFLIQETQRKEP
                     LLPASPFLLIDEVHHLPEIAEKVNDKRLSINYFHKQIQQLEEPNQLFDRIQSYLPKEH
                     EALRWLSIYREELSALCEAQICMAEGLMSLVSGKQPNGQYPEEVMMTKEMLDQLPLES
                     GTAIDHLQLFYQEIQQLQSQMRLTFEIIQESWTNQMRIDLASLYSLFDQLDKQYLLMD
                     QWLEDWRDHLIHWFVPGNTAQQMIFHLHDFQAAMLPSTVWYPRYDRILYIGGTLKIGP
                     NRHYLSKRLGIEAAPLKVIASPYDYEKQARIYVPKEAPAVHQVSNQAYIDYLETALTG
                     LIEQEDRPILVLFTSHDVLQKVYQKMHLSMLEKGREILAQGFGGSREKLLKRFILSEK
                     SVLFGADSFWEGVDLPGDALQIVVVTRLPFDNPLRPMVKARNAYLESEGINPFYQESV
                     PKAALKLRQALGRLIRTETDRGVLLILDRRLVTAKYGARIIHALPKKVPIKELAME"
BASE COUNT         1294 a          743 c          830 g         1127 t
ORIGIN      
        1 aaaaagaggt tgtgacgtgg tcacgacctc tttttgacga ttatgataca aaaaacatca
       61 tttatgaaca aaatgtgaat aacgggcgtt cttgagatat gattttaatt gggaaaaact
      121 tatcgataag gaggtcttta tgaaaaaaag actgttatgg tgtttgctgt ttctatccct
      181 aagtttaaca agtcagccag tttttgctga agaaacacag cggtctccta ttacaggcac
      241 acgtctgtac aaaaatgttt atgtacagac aacagatctt gattctgcga ttcctgaaac
      301 agaagaacgg tctttgcagc caagatttgg ttacttttgg tactgtccga cctgtgatta
      361 tgaaagttgc tggcatttct tacattctac agctgcagct tttgctttta ctcatactca
      421 gaaatatgat ggacatcatc ctgttattta tgaggtatag atatgagcaa aaaagaaaaa
      481 ctaatctcgt tgatcgtatt ggtttgcctt gttcttctgg taactgtcat aggctatcag
      541 aaataccatg agaacatcag acctactaaa gaatcttccc cagcctcgac cgttgaaaca
      601 gaaccgataa gtagtcagaa agaataattt ttttagttta aagaagaaca tcagagcaaa
      661 aaaacaccaa ctgaacaata tgtcgtttat tttaaaaaaa gttcggtttt tcactttatg
      721 taatcgctga gaaaactttt tttaataaaa aaagtcgaaa aactctagct tttttgtaaa
      781 cggtatgata tactatagga gtagtttttg ttcggtaatc tgtggtagct tgtttcaaat
      841 aaacatcttc cagatataca ccattcaagt aattgcaaaa tatgtgttga tacacaagga
      901 ggatttttat tatggaaaca ggtacagtaa aatggtttaa ctcagaaaaa ggatttggat
      961 tcatcactgc agaaaacgga aacgatgtat tcgttcattt ctcagctatc caaggcgacg
     1021 gattcaaaac tttagaagaa ggccaagcag taactttcga tgttgaagaa ggccaacgcg
     1081 gacctcaagc aacaaacgtt actaaagctt aattaaccta tactaaaaaa taaacgccca
     1141 gacatttgcg tgtctgggcg tttttgtcta ttttctttaa aaagcgatga gagtattttt
     1201 ctgcactttg tagtataatt tcaaaagaac attagaggca ggagaagagg aagatatttt
     1261 gaaaccaaga acttatgcag tcgttgatat cgaaacgact gggacgaatc caaaagaaga
     1321 tcggatcatc caatttggct gcgtcatgat tgaatcaggt cggatcactt cacgtttttc
     1381 gatcgatatt catccagggc ggaagatttc aaaacaaatc cagcatttaa cagggatcac
     1441 gaatcaacgc gtccaaaaag cgccttattt cgaagatgtc gcccaaacga tttacaatct
     1501 tttggcagac accatatttg tcgcgcataa tatttatttt gattataatt ttttgaacca
     1561 tgaactcatg cgatgcggac ttcctagttt gaagatacca ggcatcgata cagtagaact
     1621 tgcacaaatt ttcttgccga cggaaccaag ctttcgctta gctgatttgt cagaaagcct
     1681 tggatttatt catgagaatc ctcatcaggc agatagtgat gccgaagtga cgggtcaact
     1741 tcttttgctg atcgaagaac ggatgagaaa attgccaatc atcacgttag agcagattgc
     1801 gcgattaagt agacacacag gtatggatac gagtcgtttc atttatcatg tgattgaaga
     1861 aatgaaagaa aaaccagagc cacttgatcc ctcattagaa atcgtcgatg ggctagcttt
     1921 gcagaaaaaa gaagtggaac tattcacatc cgtccattat ggcgagcgga catatccaag
     1981 aaaaaaacaa gcaaaagaaa aaatgtttgg taaaacactg atgtatcgaa aagaacagaa
     2041 cagactgatg aatgcggtct acgaccattt cacaaaagat gaatcaaaag atttgatgat
     2101 cgaagcagct acaggaatgg gtaaaacaat cggttatctg ctgccattga gttatttggc
     2161 tacacctgaa aaacccgctg tcatcagtac cgtttcgcta gttttgcagc agcagatcat
     2221 agaaaaagac attccattgc tgaatcaatt gctagatcag cccatacaac ctgtaattat
     2281 caaaagctat cgccactata ttgatttaca gcgttttaaa ggcacacttg tggaaccgcc
     2341 agaacaaaaa caatatgctt tgtatcaaat ggcggtctta gtttggctga cgcaaacgaa
     2401 gaccggtgat ttggatgaat tacatttaac aaatttgaac cattcattct ttgcagatat
     2461 cgcgcacaga ggtattggat ttttagcaag aaatcagtca ttttacgaac aagactttgt
     2521 tcgtttcttg caaaagaaaa tcaggcaaag taattttttg attgtcaatc atgcgttttt
     2581 aatacaagaa acacagcgaa aagaaccatt gcttccagct agtccatttt tgttgatcga
     2641 tgaagtacat catttgccgg aaatcgctga aaaagtaaat gacaaacgat taagtattaa
     2701 ctatttccat aaacaaatcc agcaattaga agaaccaaat cagctgtttg accgaataca
     2761 aagctattta ccgaaagaac atgaagcctt acgctggtta tcgatttatc gagaagaact
     2821 ttctgcgcta tgcgaagcac agatttgtat ggcagaagga ttaatgagtt tagtgagcgg
     2881 caagcaacct aacggacagt atccagaaga agtcatgatg acaaaagaga tgttggatca
     2941 gctgccactg gaatctggga cagccattga tcacctgcag cttttttatc aggagatcca
     3001 gcagctacag tcacaaatgc gtctgacttt tgagattatc caagaaagct ggacaaacca
     3061 gatgcggatc gatttagcaa gtttatatag cttatttgat caattagaca agcagtactt
     3121 gctgatggat caatggctgg aagactggcg agatcatttg atccattggt tcgttcctgg
     3181 gaacacagcg cagcagatga tttttcatct ccatgatttt caagcggcta tgttgccgtc
     3241 gactgtttgg tatcctcgat atgatcgtat tctttatatt ggaggaacgc tgaagatcgg
     3301 ccctaatcga cactatctct ctaagcggct tggcattgaa gcagcacctc ttaaagtgat
     3361 tgcctctcct tatgattatg agaaacaggc aaggatctat gtgccaaaag aggcacctgc
     3421 cgtccatcaa gtcagcaatc aagcatatat cgattattta gagacagcac ttacaggtct
     3481 gatcgaacaa gaagatcggc caatacttgt gttattcact tctcatgatg ttttgcagaa
     3541 ggtctatcag aaaatgcatc tgtctatgct agaaaaagga cgagaaatac tagctcaagg
     3601 gtttggcgga agccgggaga agcttttgaa acgcttcatt ttgtcagaaa aaagcgtatt
     3661 gtttggcgct gacagctttt gggaaggtgt agatttgcct ggggatgctt tgcagatcgt
     3721 tgtagtcaca cgtttaccct tcgataatcc gcttcgcccg atggtcaaag ctaggaacgc
     3781 ttatttagaa tcagagggga tcaatccttt ttaccaggaa tccgtaccta aagcagcttt
     3841 gaagttacgc caagcattag gtcgcttgat aaggacggag acagatcgtg gcgtattgct
     3901 tattttggat cgaagactag tcactgcaaa atatggtgca cggatcatcc atgcattacc
     3961 gaaaaaagta ccgatcaaag agctagctat ggag
//
LOCUS       DACEEZ010000152         3915 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20313_2017
            SAMN12639966-rid9879223.denovo.158, whole genome shotgun sequence.
ACCESSION   DACEEZ010000152 DACEEZ010000000
VERSION     DACEEZ010000152.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639966
            Sequence Read Archive: SRR10023158
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3915)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3915)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741683.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741683.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:44:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,016
            CDSs (total)                      :: 2,962
            Genes (coding)                    :: 2,889
            CDSs (with protein)               :: 2,889
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 36 of 73
            Pseudo Genes (incomplete)         :: 29 of 73
            Pseudo Genes (internal stop)      :: 18 of 73
            Pseudo Genes (multiple problems)  :: 8 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3915
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639966-rid9879223.denovo.158"
                     /isolate="E20313_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(153..980)
                     /locus_tag="IWO89_002793"
     CDS             complement(153..980)
                     /locus_tag="IWO89_002793"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010294603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HAQ0457482.1"
                     /translation="MVLDFIEILKVIFLGIVEGITEWLPISSTGHMILADKFITLNMS
                     EAFKEMFFVVIQLGAILAVVVMFWNKMFPFQLKDKSQPLVKKDTFSLWFKVAVACIPS
                     GIMGILFDDYLDAYLQTPIVISIMLIFYGLLFILIENWNKKRTPTTMALSDISYKTAI
                     LIGAFQVLSLIPGTSRSGATIIGALLIGVSRVAAAEFTFFLAVPTMLGASAFKLLKFG
                     FDFTSAELLALVLGMAVAFAVSVLVIKFLMNFIKKHDFKVFGWYRIVLGILVYLLYL"
     gene            complement(980..1729)
                     /locus_tag="IWO89_002794"
     CDS             complement(980..1729)
                     /locus_tag="IWO89_002794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000933358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HAQ0457483.1"
                     /translation="MLNLISCELLKLKRSKMVLISVAGVLSTPLLMLIEALQTHFDKP
                     EIIFTLSDIYSDSVLYIMLLVNIMIYVAIAAYLFSREYTESTLKTILPIPISRTKLLI
                     GKFCTLLLWIVMLTLVTWAGIFIACGLYHAVFTLEGYSLLVAIVWLPKFLFGSILMFL
                     TVSPFVFVAMKTKGFVAPMIGSAVIVMGSAALSNQEWGALYPWTATYFLVQGKLQSTG
                     YPTLLSVSIIILVSAVGFLMTFHHFKKEDLK"
     gene            complement(1722..2639)
                     /locus_tag="IWO89_002795"
     CDS             complement(1722..2639)
                     /locus_tag="IWO89_002795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018368562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAQ0457484.1"
                     /translation="MDYIIETENLTKRYGTATVVDKVNLHVPTGKIYGLLGRNGAGKT
                     TAMKMMLQLALPTDGTVRLFGTNYKENIHTLYSKVGSIIETPGFYSNLTGYENLQILA
                     KLRGGVSKSGVEKALEVVGLHKEKRKVFSDYSLGMKQRLGIAAAIMHEPELLILDEPI
                     NGLDPIGIVEIRSFLSELSHNHGITIFISSHVLSEIEQIADIIGVMHEGHLVEEVNIS
                     ELHKRNRKYIQFDLSDSEIAGKILESHYHMTDFTVQDGTVRIYDFSQSVGEINREFAR
                     NGLLVTRIDDSEENLEDYFSKLIGGGGIA"
     gene            complement(2822..3436)
                     /locus_tag="IWO89_002796"
     CDS             complement(2822..3436)
                     /locus_tag="IWO89_002796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016897369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAQ0457485.1"
                     /translation="MEFNEKLQQLRTGKNLTQEQLAEQLYVSRTAISKWESGKGYPNI
                     ESLKCISKFFSVTIDELLSGEELITLAETENRSNLKKIYNYIYGILDMMAVAFIFLPL
                     YGNSVGGYVYAVNLLSFTATTPFNLAVYWSAFAALIVIGIAKIILTHLDKETWGGIAT
                     KCSLGLTALAICFLAAAREPYITALMFLLFVAKIVVWIKQNGTK"
     gene            complement(3426..3623)
                     /locus_tag="IWO89_002797"
     CDS             complement(3426..3623)
                     /locus_tag="IWO89_002797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000384569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAQ0457486.1"
                     /translation="MDYISVKAASEKWGISERRIQKLCEENRIDGTEKFGRAWMIPKD
                     AEKPVDGRMKTRNKQEENHGI"
     gene            3789..>3915
                     /locus_tag="IWO89_002798"
     CDS             3789..>3915
                     /locus_tag="IWO89_002798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009246558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAQ0457487.1"
                     /translation="MYQDERKLDFKPLGIAIKKAREAKGWTQEYLAQLVDLTPRSI"
BASE COUNT         1219 a          835 c          684 g         1177 t
ORIGIN      
        1 ttggtttgtg taaacgcaaa gaaacggcgg ctctgaaaat caaagccacc gtttcatagg
       61 cgtgaaaata tgtctgctgt acgacggctt ttttcttttg ccgtcgtgcg gcagattatt
      121 gcttatggaa ttgtttttct atcacattac tgttataagt acaacaagta taccaagata
      181 ccaagcacaa ttcgatacca accaaacacc ttaaaatcat gttttttgat aaagttcatt
      241 aggaatttaa ttactaagac agataccgca aatgccacag ccataccgag aacaagagcg
      301 agcagttctg cacttgtaaa atcaaacccg aattttaaca gcttaaaagc acttgctcca
      361 agcatagtag gtactgcgag gaaaaaagta aactctgctg ctgccactcg tgaaactcct
      421 atgagtaaag cacctataat cgttgctccc gaccgtgatg tgccgggtat aagtgataac
      481 acttgaaatg cccctatcaa gattgctgtt ttatagctga tgtcagaaag tgccatagta
      541 gtaggagtac gctttttatt ccaattttct atgagaatga ataacaaacc ataaaagatt
      601 aacatgattg aaatcacaat gggggtttgg aggtaagcat ccaaataatc atcaaataaa
      661 atccccataa tacctgacgg tatacaagcc actgcaacct tgaaccacaa cgagaaagta
      721 tcctttttaa caagtggctg cgatttatcc tttagctgaa atggaaacat cttgttccaa
      781 aacattacaa ccactgcgag tatagctcca agttgaataa caacaaaaaa catttcttta
      841 aatgcttcgc tcatattaag tgtgataaat ttgtccgcaa gaatcatatg tcctgtgctg
      901 ctgataggta gccactcagt aataccctca acaattccaa gaaaaataac ttttaaaatt
      961 tctataaaat caagtaccat tatttcaaat cctccttttt aaaatgatga aaggtcataa
     1021 gaaaaccaac tgctgataca agaataataa tagatacaga cagcagtgta ggatagccgg
     1081 tactctgaag tttaccctgc accaagaaat aggtggctgt ccacggatac aacgctcccc
     1141 attcttgatt tgaaagtgcg gcacttccca tgacaatcac ggcagagcca atcatcgggg
     1201 cgacaaatcc ttttgttttc atagcaacaa acacaaaagg agaaactgtt aaaaacatca
     1261 ggatactgcc aaacaaaaac ttggggagcc atactattgc cactagtaag ctatatccct
     1321 ccaatgtaaa gacagcgtga tatagcccac aagcgataaa tatacctgcc catgttacaa
     1381 gggtgagcat aacaatccaa agaagcaggg tgcaaaattt tccaattaat agttttgtcc
     1441 ttgaaatggg tatgggcaat atggttttga gtgtgctttc tgtgtactct ctgctaaaca
     1501 agtaagctgc aattgccaca tatatcataa tgtttaccag cagcatgata tacagtacac
     1561 tgtcgctgta tatgtcagac aaggtaaaga taatctccgg cttatcaaaa tgtgtttgca
     1621 aggcttctat taacatcaaa agtggggtag ataatacccc tgccacacta attagcacca
     1681 tttttgaacg ctttagtttt aataattcac aagaaataag attaagcaat accgccacct
     1741 ccaatcagtt tagaaaagta gtcctctaag ttttcctcac tatcatctat ccttgtcacg
     1801 agcagtccat ttcgtgcaaa ttctcgattg atttctccaa cactttggct aaagtcataa
     1861 attctcaccg taccgtcctg cactgtaaaa tccgtcatgt ggtagtggct ttctaaaatc
     1921 tttccagcta tctcactgtc agataaatca aattgaatgt attttcgatt ccttttgtga
     1981 agctcggata tattcacttc ctctactaaa tgcccctcgt gcataacgcc gataatatct
     2041 gctatctgtt caatctcgct taaaacatgg cttgagataa aaatggtaat gccatgattg
     2101 tgactaagtt cagataaaaa tgagcgtatt tcaactattc caatggggtc aagtccgtta
     2161 atcggttcgt caagtattaa aagctccggc tcgtgcataa tggcggcggc aataccaagc
     2221 cgttgcttca ttccgaggga ataatcggaa aagacttttc ttttctcttt atgcagcccc
     2281 acaacttcaa gagctttttc tactccactt ttagatactc cccctcgcag tttagcgaga
     2341 atttgcaaat tctcataccc tgttaaatta ctgtaaaatc ccggtgtttc gataatagag
     2401 cctactttgc tataaagggt atggatattt tccttatagt ttgtgccaaa caagcgtacc
     2461 gttccatccg ttgggagagc aagctgcaac atcattttca ttgctgtggt tttgcctgct
     2521 ccgtttctgc cgagcaaacc ataaattttg cccgttggca catgaagatt gaccttatcg
     2581 acaacggtgg ctgttccgta tcgcttcgta agattttctg tttcaatgat ataatccata
     2641 tttgattttc ctttccgttg gttgctgttc ttgttttcac tgaccacaat tttattatca
     2701 caggagctat atcaaaagcc aagaaaccta ccgtcacatc gcctacatct atcatgtcaa
     2761 catgaaaaaa agccccgaca cttatcgtgt cagagcctat ttcaagggat ttatatcgtt
     2821 tttactttgt tccgttctgc ttaatccaaa caactatttt tgcgacaaac agcagaaaca
     2881 taagtgctgt tatatatggt tctcttgctg ctgctaagaa acagatagcc aaagcagtga
     2941 gtccaaggga gcattttgta gcaatgccgc cccatgtttc tttatctaaa tgggtaagta
     3001 ttattttagc aatccctatt acaatcaagg cggcaaaagc actccagtag actgcaagat
     3061 tgaatggtgt ggtggctgta aatgatagta gattgaccgc ataaacataa ccgccaacag
     3121 agtttccata taacggaaga aatataaacg caactgccat catatccaat attccataaa
     3181 tatagttgta aatctttttc aagttggaac gattttcagt ttcggcaagt gtaatcagtt
     3241 cttcgcccga tagtagttca tctatggtca cagaaaagaa tttagaaata cacttgagcg
     3301 actcaatgtt agggtaaccc ttgccgcttt cccattttga aatggctgtt cttgatacat
     3361 ataattgctc cgcaagttgt tcctgcgtta agttctttcc agtcctaagc tgttgtagct
     3421 tttcattaaa ttccatgatt ctcctcctgt ttgttccttg ttttcatacg tccatcaacg
     3481 ggtttttctg catcttttgg tatcatccat gcacgaccaa atttttcagt tccgtcaatg
     3541 cgattttcct cacataattt ttgtatccgt ctttccgata tgccccattt ttcagaagcg
     3601 gctttcacag aaatgtaatc cataatatca acttcctttc gcaaagagta tataattatt
     3661 atatacggaa tgacgaataa tagcattttt ctctccacta atacgaaatt atacactact
     3721 aaacataaaa tcatacttcg ttcaaatatc atcacccgaa atgtacaatg atatagggtg
     3781 atattaaaat gtaccaagat gaaagaaaac ttgattttaa gccgttaggt atagcgataa
     3841 aaaaagctcg ggaagcaaaa gggtggacac aggaatatct tgcccaactg gtagacctta
     3901 cgccacgctc tatta
//
LOCUS       DACEEZ010000153         3898 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20313_2017
            SAMN12639966-rid9879223.denovo.159, whole genome shotgun sequence.
ACCESSION   DACEEZ010000153 DACEEZ010000000
VERSION     DACEEZ010000153.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639966
            Sequence Read Archive: SRR10023158
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3898)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3898)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741683.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741683.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:44:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,016
            CDSs (total)                      :: 2,962
            Genes (coding)                    :: 2,889
            CDSs (with protein)               :: 2,889
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 36 of 73
            Pseudo Genes (incomplete)         :: 29 of 73
            Pseudo Genes (internal stop)      :: 18 of 73
            Pseudo Genes (multiple problems)  :: 8 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3898
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639966-rid9879223.denovo.159"
                     /isolate="E20313_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            <1..352
                     /locus_tag="IWO89_002799"
     CDS             <1..352
                     /locus_tag="IWO89_002799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000997693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="HAQ0457488.1"
                     /translation="SKEVALEARGTFAEKWKTSYPKVVESILSNDHLLTFYDFPLAIR
                     KSIYSTNLIESFNKQIKKYSHRKEQFQNEESMERFLVSSFDTYNQKFLGRSHKGFQQA
                     EGELEQMLSQLIEN"
     gene            486..2675
                     /locus_tag="IWO89_002800"
     CDS             486..2675
                     /locus_tag="IWO89_002800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000470930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HAQ0457489.1"
                     /translation="MFISTLQIRNFRNYESEIFRFDNETNTIIGENDSGKTNALTALR
                     ILLDDSYYYSSKTLKESDFFHGIQNGWQGHWIIISATFEGISEEEFDNEICASISLNS
                     ESQTILEELISNADKGVGSISLFIRPNKAIRKQLFDISTELDSHQFNEFRNSIRLSDY
                     EFYYTSKSNLNFCINENYDKLVGRLNDNIASNPEEDDEALLGSRIDMSDIFKHISVVY
                     VDALRDVLREMKNNRNPVKRIMETIESKISSDNVESLKTIIQQLNETITSVPEIRKIG
                     ENINRQLNSIIGSVYSPNLLLSSTMSDDMGSLAKFLSMKPEQNIDLDLLGLGHLNMIY
                     LALKIVEFEACRSRELLNIMLIEEPEAHIHHHIQKTLFEGLNLQKNYTQILMTTHSVH
                     LAEVSEISRMNVLKSFNGKSIVMNPANNLNSFAKEKLKKNNLNLINAVERYLDVKRNG
                     LLFSKGVILVEGDAEEILIPQIVKKLFGINLDELGIGLINVGSTSFEYIASLFDDDRI
                     QRKCAIVTDLDEQAIPSEHRLYKSTAQEKGQARKEKLSSLFNENNWVNCFYANTTFEI
                     EFLDVNKENILTYVSPIIDDAFKDKKTITAYKEEIQSEQYLERNLAMLKLANHVGKGW
                     FAIELGEKIDFLIKIPKYILNAIAFACQEIVDLSIYKKILLHTIDLYEETTELSALSA
                     SLKTSDTADLNIAIISLLEYFGKDEDVVYLINQIENYTKILYAEVSK"
     gene            2672..>3898
                     /locus_tag="IWO89_002801"
     CDS             2672..>3898
                     /locus_tag="IWO89_002801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000181735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase"
                     /protein_id="HAQ0457490.1"
                     /translation="MTNIFEKIVNDLNLEQKDVVESEDNLYVNACPGSGKTRVLTRKI
                     AYQAIKHDGSLKRIIAITYTNRAAEEIKERLSLLSIDDDINIWVGTIHQFCLEFIIYP
                     FKMNLSRVSKGFTIIDDYTQRAYVSQINNMLNLNLKTYELNKIDLSLSHDFKINEVQY
                     TQVSEMYHTMLEKNKEIDFDMILLVAYKILTVNSNVARNIASNIRAIYVDEFQDTREL
                     QYNVIAKLLQNNPQIQSMFVGDIDQAIYGSLDGIAKSVEELEMLTSHTFQSKTLHGCY
                     RSNQRLVDFYSNFQSCPYEIESRGNNKNDRGFISYDCKVTKEDLPNIIKNIIKEKIES
                     GIHEKDICVLAPQHYPLFSLGEYLKKELPYCNFDAINISPIKVNNHSLFFKLAILYFT
                     ESGEKVRRRKLIANDIL"
BASE COUNT         1457 a          551 c          659 g         1231 t
ORIGIN      
        1 atctaaagag gtggcgttgg aagcacgtgg tacttttgcg gaaaaatgga aaaccagcta
       61 tccaaaagtg gttgaatcga ttctttcgaa cgatcacttg ctcactttct atgatttccc
      121 cttggccata cgcaagagta tttattctac gaacttgatt gaatccttta ataaacaaat
      181 caagaaatac agccaccgca aggaacagtt ccaaaatgaa gagtcgatgg aacgtttctt
      241 agtctcgtct tttgatactt acaaccaaaa attcctaggt cgcagtcata aaggtttcca
      301 acaggccgaa ggcgagctcg aacaaatgct aagccaactg attgagaatt agaaacaatc
      361 tgcagtaggg aagaatcatt tacacaaaat tattgacgct cccttataat gagaactttt
      421 actaacataa tttatgataa acttactata taatcatatt aattaaatag ttaggagatc
      481 tgcttatgtt tatatctact cttcaaattc gaaattttag aaattatgaa tccgaaatct
      541 ttcgattcga taatgagaca aatactatta taggtgagaa tgattcaggg aaaaccaatg
      601 ctcttactgc attgagaatc ttattagatg atagttatta ctatagtagt aaaacactaa
      661 aagaatcaga tttttttcat ggaattcaaa atgggtggca agggcattgg attattatct
      721 ctgcgacatt tgaaggaatc agcgaagaag aatttgacaa tgagatttgt gcttccattt
      781 ctttaaattc tgaaagtcaa acaatcttag aagaattgat ttcaaatgca gataaaggtg
      841 taggtagtat ttctctattt attagaccta acaaagctat tagaaagcaa ttatttgata
      901 tttccactga attagactct catcaattta acgaatttag aaattctatt cgtctatccg
      961 actatgaatt ttactacaca tctaaatcaa atctaaattt ctgtatcaat gaaaattatg
     1021 ataaattggt aggaagactc aatgacaata tagctagtaa tcctgaagaa gacgatgaag
     1081 ctctgttggg ctcaagaatt gacatgtcag atattttcaa gcatatttcg gttgtttatg
     1141 ttgacgctct tcgagatgtt cttagagaaa tgaaaaacaa tcgaaaccca gtcaaaagaa
     1201 tcatggaaac aattgaatca aaaatatcat cagataatgt tgagtcatta aaaacaatta
     1261 tacagcaatt aaatgagact attaccagtg ttcctgaaat taggaaaatt ggcgaaaata
     1321 ttaatcggca actaaatagc ataattggtt cagtgtattc tcctaatctt cttctttcct
     1381 caactatgtc agatgatatg ggttctttag caaaatttct ttcaatgaag ccagaacaaa
     1441 atatcgactt agacttacta gggttaggac atctaaatat gatttattta gctttgaaaa
     1501 tagtcgaatt tgaagcgtgt cgttcaagag aactacttaa tattatgttg attgaagaac
     1561 cagaagctca tattcatcat catattcaaa aaactctgtt cgaaggatta aatcttcaaa
     1621 aaaactatac tcagattctc atgaccacac attctgtaca tttagctgaa gtgtctgaaa
     1681 tatctcggat gaatgtatta aaatcgttta atggcaaatc tatcgttatg aatccagcaa
     1741 ataatctgaa ctcatttgct aaagaaaagt taaaaaagaa caatctaaat ctaataaacg
     1801 cagtagagag atatttagat gttaaaagaa atggattgct tttttctaaa ggtgttatct
     1861 tagttgaagg agatgctgaa gaaatactta ttcctcaaat agtaaaaaaa ttatttggta
     1921 taaacttaga cgaacttgga ataggtttga ttaatgttgg tagtacttcg tttgaatata
     1981 tcgctagctt gtttgacgat gatagaatac agagaaagtg tgctattgta actgatttag
     2041 atgaacaggc aatcccatca gaacatcgat tgtataaaag cactgcacaa gagaaaggtc
     2101 aagcaagaaa agaaaaatta tcctcccttt ttaatgaaaa caattgggtt aattgttttt
     2161 atgctaatac aacatttgaa attgagtttt tagatgtgaa taaagaaaat attctaactt
     2221 atgtcagtcc aattatagat gacgcattca aagataaaaa aacaataact gcttataaag
     2281 aagaaataca aagtgaacaa tatttagaaa gaaatttagc gatgttaaaa ctagccaatc
     2341 atgttggtaa ggggtggttt gcaattgagt taggtgaaaa aattgacttt ttaatcaaaa
     2401 ttcctaagta tattcttaat gctattgctt ttgcatgtca ggaaatagta gatctctcaa
     2461 tttataaaaa aattctactc catacaatcg acttgtacga ggaaactaca gaattgtcag
     2521 ctttatcagc tagtttaaaa acaagtgata cagctgattt gaatatagca ataatttctt
     2581 tattagagta ctttggtaaa gatgaggatg ttgtgtatct aatcaatcag atagaaaatt
     2641 acacaaaaat actttatgct gaggtttcaa aatgacaaat atcttcgaaa aaatcgtaaa
     2701 tgatttaaat ttagaacaaa aagatgttgt ggaaagtgaa gataaccttt atgtaaatgc
     2761 atgtcctggg agtgggaaaa caagggtact tacaaggaaa attgcttacc aagcaattaa
     2821 acacgatgga tcactaaaaa gaataattgc tatcacatac actaatagag ctgctgaaga
     2881 aattaaggaa cgcctaagtt tacttagtat agatgatgat attaatattt gggtggggac
     2941 aatacatcag ttttgtcttg aatttattat ttatcctttt aaaatgaatc tctcaagagt
     3001 atcaaaagga tttacaatta ttgatgatta tactcaaaga gcttacgtca gccaaattaa
     3061 taatatgctg aatttaaatc tcaaaacgta tgaactgaac aaaattgatt tgtcactttc
     3121 tcatgatttt aaaattaatg aagttcagta tactcaagtt tctgaaatgt atcacactat
     3181 gttggaaaaa aataaagaaa ttgattttga tatgatttta ctagtagcgt ataaaatttt
     3241 aactgtaaac agtaacgttg cacgtaatat tgctagtaat ataagggcga tttatgtgga
     3301 tgaatttcaa gatactagag aattacaata caatgtaata gctaaattac ttcaaaacaa
     3361 ccctcaaatt cagtcaatgt ttgttggaga tattgaccaa gcaatttatg gatcattaga
     3421 tggtatagct aaatcagttg aagaactaga aatgctaact agtcacactt ttcaatcaaa
     3481 gacgctacat ggctgttaca ggtctaatca gagattagta gacttctact ctaattttca
     3541 gagttgtcct tacgagattg aaagcagagg gaataataaa aacgaccgtg gttttataag
     3601 ttatgattgc aaagttacta aggaggattt acccaacata ataaaaaata taataaagga
     3661 aaagattgaa agcggcattc atgaaaaaga tatttgtgta cttgctcctc aacactatcc
     3721 tctgttttca ctgggtgagt atttaaagaa agaattaccg tattgcaatt ttgatgctat
     3781 aaatatatca ccaattaagg taaataatca tagtttattt ttcaagttag caattttgta
     3841 ctttactgaa tcaggtgaaa aggttcgaag aagaaagcta attgcaaacg atatttta
//
LOCUS       DACEEZ010000154         3896 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20313_2017
            SAMN12639966-rid9879223.denovo.160, whole genome shotgun sequence.
ACCESSION   DACEEZ010000154 DACEEZ010000000
VERSION     DACEEZ010000154.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639966
            Sequence Read Archive: SRR10023158
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3896)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3896)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741683.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741683.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:44:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,016
            CDSs (total)                      :: 2,962
            Genes (coding)                    :: 2,889
            CDSs (with protein)               :: 2,889
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 36 of 73
            Pseudo Genes (incomplete)         :: 29 of 73
            Pseudo Genes (internal stop)      :: 18 of 73
            Pseudo Genes (multiple problems)  :: 8 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3896
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639966-rid9879223.denovo.160"
                     /isolate="E20313_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            <1..531
                     /locus_tag="IWO89_002802"
     CDS             <1..531
                     /locus_tag="IWO89_002802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HAQ0457491.1"
                     /translation="TVPESSTTEESTTPAPTTPSTDQSVDTGNGTGSSTPAPTPTPTP
                     EQPKPVTPAPAPSGSVNGAAIVAEAYKYIGTPYVWGGKDPSGFDCSGFTRYVYMQVTG
                     RDIGGWTVPQESAGTKISVSQAKAGDLLFWGSQGGTYHVAIALGGGQYIHAPQPGESV
                     KVGSVQWFAPDFAVSM"
     gene            complement(599..2398)
                     /locus_tag="IWO89_002803"
     CDS             complement(599..2398)
                     /locus_tag="IWO89_002803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M3 family oligoendopeptidase"
                     /protein_id="HAQ0457492.1"
                     /translation="MSYSLNWNLDSIFSGGSHSDALNQRMKQLEDQMNEYYHRVTKWS
                     PSSDNAEQLNAILQLQETITNGFTQCSSYITALLSANVNDSDAKVLSGKLYAMLPRLQ
                     SAETVLSKKFAEISDNDWNQMLSQSSFETIAFRLNEIRRDGSQLLSEAEENIINTLSL
                     DGLNAWSSHYDTIVASISIPFEQDGQVVELSAGQAFNKMMGDPDPKVRETLFAAWEEA
                     WKEKAPLFTDTLNHLDGFRLSDYELHGVTDFLQKPLEYNRLKKETLSVMWDTIQKNKQ
                     PLVNYLTRKANLFGKEKMEWQDQDAPIILGDLKEKTFSFDEAAAFIIENFNKFSPKMA
                     KFAQSAFEKSWIEAEDRPGKRPGGYCTELPETKESRIFMTYSNSVNEVATLAHELGHA
                     FHSSTMWDLPSLNREYAMNVAETASTFAELIVADATLKVAKTKEEKINLLDTKLQNAL
                     AMFMNIHSRFIFENRFYEARQEGLVSEDKITEMMVEAQKEGYQGALATYHPYFWAAKL
                     HFFIDDVPFYNFPYTFGYLFSLGIYAYANQKGANFEEEYIHLLRDTASMTTEDLAKKH
                     LNVDLTQPDFWQAGIDMVTEDIQTFMELTEEFI"
     gene            2521..3027
                     /locus_tag="IWO89_002804"
     CDS             2521..3027
                     /locus_tag="IWO89_002804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HAQ0457493.1"
                     /translation="MNSSKKQMKNGNIYLVIALIVVIILFYSSSQTYGQQSQVSRLES
                     WLPNQPFKDTLAGIRFNYGGSMVSINHLGYAKFLEFFLRKGAHFGTYFILGGSLFLGV
                     FPKLKIWWLTAILAWLSATGYAGLDEFHQMMTGGRTPMFQDVMLDSIGALTAVILCVL
                     FAAFKKRL"
     gene            3106..3828
                     /locus_tag="IWO89_002805"
     CDS             3106..3828
                     /locus_tag="IWO89_002805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HAQ0457494.1"
                     /translation="MSGHSKWHNIQGRKNAQDAKRGKVFQKLSREIYMAAKAGGPDPS
                     MNPALRLVVDKAKAANMPNDNVERAIKKATSAVDETNYDEITYEGYGPGGVGILVHAL
                     TDNRNRTATNVRVAFTRNGGSLGETGSVSYLFDRKGYIAIERDGLTVDEETMFEDVLE
                     AGAEDLQTSPEVFEIYTAPEDFAAVRDELEKEFTLAQAELTMIPQTTIDLNEEQKEQL
                     ERLVDKLEDDDDVSEVFTAAEM"
BASE COUNT         1170 a          730 c          820 g         1176 t
ORIGIN      
        1 acagtgcctg aatcttcaac aacagaagaa tcaacaacac ctgcgcctac aacaccatca
       61 acagatcaaa gtgttgatac aggaaacggc acaggaagta gtactccggc tccaacgcca
      121 acaccaacac ctgaacaacc aaaacctgta acacctgctc cagctccttc tggaagtgtg
      181 aatggtgcag caatcgtagc agaagcttac aaatatattg gtactccata tgtttggggc
      241 ggtaaagatc caagtggctt tgactgctca gggttcacac gctatgttta catgcaagta
      301 actggtcgtg acatcggtgg ttggacagtt cctcaagaat cagctggaac aaaaatttca
      361 gtttctcaag caaaagctgg agacttgtta ttctgggggt cacaaggcgg aacttaccac
      421 gtagcgattg ctttaggcgg aggacaatat atccatgctc ctcaaccagg cgaaagcgta
      481 aaagttggtt cagttcaatg gtttgcccct gactttgctg tcagcatgta agaatgatta
      541 atcaaaaaga gttagcccat tctggactaa ctcttttttt attgtgataa aatagaaatc
      601 agataaattc ttctgtcaat tccatgaacg tttggatatc ttcagtcacc atgtcgatac
      661 ctgcttgcca aaaatctggt tgggtaagat cgacattcaa atgtttcttc gcaagatctt
      721 ccgtcgtcat tgaggcagta tcgcgtagta aatggatata ttcttcttca aaatttgctc
      781 ccttttggtt ggcgtaagca tagatgccca aactaaagag gtagccaaat gtgtatggga
      841 agttataaaa cggtacgtca tcaataaaga aatgcaattt tgcagcccag aaatatggat
      901 ggtaagtagc taaagctcct tgatagcctt ctttttgcgc ctcaaccatc atctctgtga
      961 tcttatcttc tgagactaat ccttcttgcc gtgcttcata gaatcgattt tcaaaaatga
     1021 atcgggaatg gatattcatg aacatagcta aagcattttg cagttttgta tctagcaaat
     1081 tgattttttc ttcttttgtt tttgcgactt ttaatgtagc atcggctacg atcagttctg
     1141 cgaatgtact tgctgtttct gcgacattca tggcatattc gcgatttaaa gaaggtaaat
     1201 cccacatcgt gctgctatgg aaagcatgtc ctagttcatg agcaagtgtc gcaacctcat
     1261 taacagaatt actgtaggtc ataaagatcc ttgattcttt tgtttctggt aactcagtac
     1321 aataaccacc aggacgtttc ccaggacgat cttctgcttc gatccagctc ttttcaaaag
     1381 cagattgggc aaatttagcc atttttggac tgaatttgtt aaagttttcg ataataaatg
     1441 ctgctgcttc gtcaaaacta aaggtttttt ctttcaaatc gcctaaaata ataggtgcat
     1501 cttgatcttg ccattccatt ttttctttac cgaaaagatt agcttttcta gtcaagtaat
     1561 taacaagggg ttgtttgttt ttctggattg tatcccacat gacagacaat gtttctttct
     1621 tcaaccgatt gtactctaat ggtttttgta gaaaatctgt gacaccatga agttcatagt
     1681 cagaaagtct aaaaccatct aaatgattca aagtatccgt gaagaggggt gccttttctt
     1741 tccaggcttc ttcccatgct gcgaaaagtg tttctctgac ttttggatct ggatcgccca
     1801 tcatcttatt gaaggcttgt ccagcagata attcgactac ttgtccatct tgttcgaagg
     1861 gaatcgaaat gctggctaca atcgtatcat aatgactgct ccaagcattt agaccatcta
     1921 aagaaagcgt atttataatg ttttcttcag cttctgataa taattgtgag ccatcacgac
     1981 gaatctcgtt taaacgaaaa gcaattgttt caaacgaaga ttgagaaagc atctgattcc
     2041 aatcattgtc agatatttct gcaaatttct ttgacaatac agtttcggct gattgcagac
     2101 gaggcagcat tgcgtaaagc ttgcctgata agacctttgc atctgaatca ttgacattgg
     2161 cagaaagcaa tgcagtaata tagctgctgc attgggtaaa accattcgta atcgtttctt
     2221 gcaattgaag aatagcgttt aactgctctg cattgtcaga actaggtgac cattttgtta
     2281 cgcggtgata atactcgttc atttggtctt ccaattgctt catccgttgg ttcaaagcat
     2341 cagaatgact gcctccggaa aaaatagaat ctagattcca atttaatgaa taactcatga
     2401 aaaaactccc ttcatattgt aatacaaaat taagtatagc acttttttat ggaagaagtc
     2461 tctatgaaaa tgattgttgt ttcttcaaaa aaagtctaag ataaaataca gaggtgactt
     2521 atgaattctt caaaaaaaca aatgaaaaac ggaaatattt atttagtaat cgcactgata
     2581 gtcgttataa tcttatttta cagttcttct caaacttacg gacagcaatc acaagtttcg
     2641 cgattagaaa gctggctgcc taatcagcca tttaaagaca cattagcggg tatacgtttt
     2701 aattatggtg gaagtatggt gagtatcaac catctgggct acgccaaatt tttagagttc
     2761 tttttaagga agggcgcaca tttcggtacc tattttattt tgggaggcag cttgttttta
     2821 ggtgttttcc ccaagctaaa aatttggtgg ctgacagcga tccttgcttg gctttcagca
     2881 acagggtacg caggtttgga cgaattccat cagatgatga ccggtggacg aacacctatg
     2941 ttccaagatg tcatgttgga ttcgatcggt gctctgactg ctgtgatatt atgtgtgctt
     3001 tttgcggcat tcaagaagcg tttatagatt ggctgtggct ttgttttttt gtgaaatagt
     3061 actagaatag aagtgtattc tcaaaagaac aagggaggaa aaatcatgtc aggacatagt
     3121 aaatggcata atatccaagg aagaaaaaat gcccaagatg caaaacgagg gaaagtcttt
     3181 cagaaacttt ctcgtgaaat ttatatggca gctaaagcag gcggtcctga tccatctatg
     3241 aatccagctt tgcggcttgt tgtggacaaa gcaaaagctg caaatatgcc taacgacaat
     3301 gtggaacggg cgattaaaaa ggctacgagt gccgtagatg aaacgaatta cgatgaaatc
     3361 acgtatgaag gatatggtcc gggtggagta ggaatcttag tccatgcatt gacagataac
     3421 cgtaatcgga cagcgacaaa tgtccgagta gcttttactc gaaacggtgg atcactggga
     3481 gaaacgggtt cagtcagtta cttgtttgac cgcaaaggat atatcgctat tgaacgcgat
     3541 gggttgactg ttgatgaaga gacgatgttt gaggatgtat tagaggcggg ggcagaagat
     3601 ctgcaaactt ctcctgaagt attcgagatt tatactgcac cagaagattt tgctgctgta
     3661 cgagatgagt tggaaaaaga gttcactttg gcgcaagcag aattgacgat gattccacaa
     3721 acgacgattg atttaaatga ggaacaaaaa gagcaattgg aacgtctagt agataagcta
     3781 gaagacgatg atgatgtttc agaagtcttt actgctgctg aaatgtaagt agcacattgt
     3841 tagataataa aaagaaagca cctgtaccaa tcgtacgggt gctttctttt tatata
//
LOCUS       DACEEZ010000155         3712 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20313_2017
            SAMN12639966-rid9879223.denovo.161, whole genome shotgun sequence.
ACCESSION   DACEEZ010000155 DACEEZ010000000
VERSION     DACEEZ010000155.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639966
            Sequence Read Archive: SRR10023158
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3712)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3712)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741683.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741683.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:44:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,016
            CDSs (total)                      :: 2,962
            Genes (coding)                    :: 2,889
            CDSs (with protein)               :: 2,889
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 36 of 73
            Pseudo Genes (incomplete)         :: 29 of 73
            Pseudo Genes (internal stop)      :: 18 of 73
            Pseudo Genes (multiple problems)  :: 8 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3712
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639966-rid9879223.denovo.161"
                     /isolate="E20313_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(161..2158)
                     /gene="tkt"
                     /locus_tag="IWO89_002806"
     CDS             complement(161..2158)
                     /gene="tkt"
                     /locus_tag="IWO89_002806"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HAQ0457495.1"
                     /translation="MFDKIDQLGVNTIRTLSIEAVQKANSGHPGLPMGAAPMAYALWT
                     KHLKVNPTTSKNWADRDRFVLSAGHGSAMLYSLLHLAGYQVTIDDLKQFRQWDSKTPG
                     HPEVHHTDGVEATTGPLGQGIAMAVGMAMAEAHLAATYNKENFNVMDHYTYAICGDGD
                     LMEGVSQEASSMAGHMKLGKLIVLYDSNDISLDGPTSKAFTENVGARYEAYGWQHILV
                     KDGNDLEAISKAIEEAKAETDKPTLIEVKTVIGYGAPKEGTSAVHGAPLGEDGIKMAK
                     EVYGWEYPDFTVPEEVAARFHQTMIEEGQKAEDAWNEMFANYKKAYPELAQQFEDAFD
                     GKLPENWDAELPTYEVGSSQASRVSSKEVIQELSKAIPSFWGGSADLSGSNNTMVAAD
                     KDFTPEHYEGRNIWFGVREFAMASAMNGIQLHGGTRIYGGTFFVFVDYLRPAVRLAAI
                     QHTPVVYVLTHDSVAVGEDGPTHEPIEQLASVRCMPGVQVIRPADGNETRAAWKVAME
                     TTDAPTILVLSRQNLPVLPSTKEVADEMVKKGAYVLSPSQGETPEGILIATGSEVDLA
                     VKAQKELAEKGKDVSVVSMPSFDLFEKQSSEYKESVLPKSVKKRVAIEAAASFGWERY
                     VGTEGATITIDHFGASAPGTKILEEFGFTVENVVNTYNQLS"
     gene            complement(2260..2511)
                     /locus_tag="IWO89_002807"
     CDS             complement(2260..2511)
                     /locus_tag="IWO89_002807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF896 family protein"
                     /protein_id="HAQ0457496.1"
                     /translation="MLSPEKIERINELARKKKENGLSKAEEQEQINLRKEYLEAFRSG
                     MRHHIEGMKVVDPEGNDVTPEKLKEIQKKKGLHNRNDNF"
     gene            2658..3281
                     /gene="lexA"
                     /locus_tag="IWO89_002808"
     CDS             2658..3281
                     /gene="lexA"
                     /locus_tag="IWO89_002808"
                     /EC_number="3.4.21.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor LexA"
                     /protein_id="HAQ0457497.1"
                     /translation="MARQTESRQIEVLRYIHEQVTQKGYPPTVREIGEAVQLSSTSTV
                     HGHLARLEKKGFIQRDPTKPRAIELTKQGLEKIGIRPTTIPVLGVVTAGEPILAVEDA
                     SDFFPIPPNLSSEEGSLFMLTIRGDSMINAGILDGDNVIVRKQETAQNGDIVIAMTAE
                     DEATCKRFYKEKNFFRLQPENDLLEPIILDQVSILGRVVGLYRSHIY"
     gene            3461..3679
                     /locus_tag="IWO89_002809"
     CDS             3461..3679
                     /locus_tag="IWO89_002809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0457498.1"
                     /translation="MKSMRRLGLVLTVLFLGIIIGNFGIRPALLVFVPLFTLWFMLWD
                     EKKYRQSERRRIHEDHFYHEPYYRSRQS"
BASE COUNT         1025 a          796 c          688 g         1203 t
ORIGIN      
        1 tcaagaaaca caaactaatc cgaagaattc tttgttttgt catttaaatg taacatagcc
       61 gaaaacgaaa agtaatattg atttattctt ctaacacata aagaacagct gtctccaact
      121 tgcagacagc tgttcttatc agtcaaacaa ttgcttggat ctatgatagt tgattgtacg
      181 tattcacgac attttctact gtaaatccaa attcttctaa aattttagtg ccaggggcag
      241 atgcaccgaa atgatcgatt gtaatcgttg cgccctctgt tcctacgtag cgttcccatc
      301 caaatgatgc agcagcttcg atagccacac gtttcttcac tgatttagga agtacacttt
      361 ctttatattc agaagattgt ttttcaaaga ggtcaaagct tggcatagaa acaacagaaa
      421 catctttgcc tttttcagcc aattcttttt gagctttcac agccaaatca acttcagaac
      481 ctgttgcaat caaaatacct tctggtgttt ctccttgtga tggagaaaga acatacgcgc
      541 cttttttcac catttcatct gctacttctt ttgtagaagg caatactgga aggttttgac
      601 gactcaatac aaggatagta ggcgcatctg ttgtctccat cgctactttc caagcagcac
      661 gtgtttcatt tccgtctgcc ggacgaatca cttgaacacc aggcatgcag cgaacagatg
      721 ctagttgttc aatcggttcg tgcgttggtc cgtcttctcc gacagcaacc gagtcatgtg
      781 ttaagacgta aacaacagga gtatgttgga ttgctgctaa acgaactgct ggtcttagat
      841 aatcgacaaa gacaaagaat gttccaccgt agatacgagt tccgccatga agttggatac
      901 cattcatagc cgaagccatt gcaaattcgc ggacaccgaa ccagatgttg cgtccttcat
      961 aatgttcagg agtgaaatct ttatctgcag ccaccattgt attattggaa cctgacaagt
     1021 ccgctgaacc accccagaag cttggtatag ctttcgataa ttcttggatg acttctttac
     1081 ttgaaacacg gcttgcttga ctgcttccaa cttcataagt tggcaattct gcatcccagt
     1141 tttctggtag tttgccatca aaagcatctt caaattgttg tgctaattca ggataagctt
     1201 ttttataatt ggcaaacatt tcattccatg catcttcagc tttttgtcct tcttctatca
     1261 tcgtttgatg gaagcgggca gctacttctt ctggaacagt gaaatctgga tactcccaac
     1321 cataaacttc tttagccatc ttgatgccat cttcaccaag aggagcacca tgaacagcag
     1381 atgttccttc tttaggagca ccatagccga tgactgtttt tacttcgatc aatgttggtt
     1441 tgtctgtttc agcttttgct tcttcaatcg ctttagagat tgcttctagg tcatttccgt
     1501 ctttcactaa gatatgttgc cagccatatg cttcatagcg agcaccaacg ttttctgtga
     1561 aagcttttga agttggaccg tctagtgaaa tatcgtttga atcgtataaa acgatcaatt
     1621 tccctagttt catatgacct gccatcgaac ttgcttcttg agatacccct tccatcaagt
     1681 ctccgtcacc gcatattgca tacgtatagt gatccatcac attaaagttt tctttgttat
     1741 acgttgctgc aaggtgcgct tctgccattg ccataccgac agccattgca atcccttgtc
     1801 ctaatggccc tgtagtggct tctacaccat ctgtatgatg tacttcagga tgtcctggtg
     1861 ttttagaatc ccattgacgg aattgtttca aatcatcgat tgtcacttga tagccagcta
     1921 aatgaagcaa gctatacaac attgctgacc catgtcctgc cgaaagaacg aaacggtcgc
     1981 ggtcagccca gttttttgat gtcgttggat ttactttcag gtgttttgtc cataacgcat
     2041 atgccatagg tgctgcgccc atcggcaatc caggatgtcc tgagtttgct ttttggactg
     2101 cttcaatact taatgtacga attgtgttaa cgccaagttg gtcaattttg tcaaacaaaa
     2161 taacccctcc tattaattta ggtttttttt acctttattt cttacacttt cattctataa
     2221 gatgtcacac gaaaaagcaa tctttttcag tattttttac taaaagttgt catttcggtt
     2281 gtgtaagcct tttttctttt ggatttcttt taatttttcc ggggtcacgt cattgccctc
     2341 aggatcgacg actttcattc cttcgatatg atgacgcata ccggaacgaa aagcttccaa
     2401 atactctttg cgcaagttga tttgttcttg ttcttcggct tttgagagac cattttcctt
     2461 ctttttacgt gcaagttcat tgatccgctc tattttttca ggtgacagca tgctcagccc
     2521 tccttttact ctccttcata ttatataaaa actgaaaaaa aaacaactag ataaactaaa
     2581 taaaatacga acattcgttt gatttagcgc tttttatatg gtactattta tttatcaagt
     2641 gaaagaaggt gtaccttgtg gcccgacaaa ctgaatcaag acaaatcgaa gtcttacgat
     2701 atatccatga acaagtaacc caaaaaggat acccgccgac tgtccgtgaa atcggggaag
     2761 ctgttcagct ttcttctact tctactgttc acggtcatct tgcacggctt gaaaaaaaag
     2821 ggtttatcca aagagatccg acgaaacctc gtgcaatcga gttgacaaaa caaggtttag
     2881 aaaagattgg gattcgtcca acaactattc cagtacttgg cgtcgtaaca gctggagaac
     2941 ccattttagc agtagaggat gcttcagact tctttccaat ccctcctaat ctttcttccg
     3001 aagaaggcag tctctttatg ctgacaattc gtggagatag tatgatcaat gctgggatat
     3061 tggatggtga caacgtgatc gttcggaagc aagaaactgc tcaaaatggc gatattgtca
     3121 ttgcaatgac tgccgaggac gaagcgactt gcaaacgatt ctataaagaa aaaaactttt
     3181 tccgtctgca gcctgaaaac gacttgctcg aaccaatcat cttagatcaa gtatctatat
     3241 taggacgagt agtaggactt tatcgtagtc atatctatta attgctacta attaaaagaa
     3301 aactttttaa aaagagtttg agacgttggt ctcaaactct tttttcgccc gggttcttct
     3361 tctttcaaat ttttatcact tccctcttta caaaccgaac aaatattcgt atactaaata
     3421 tacgaacaat tgttcgcata aattaaagag gtgagaattc atgaaatcaa tgagacgtct
     3481 tggcttagta ttaactgttt tgtttttagg tattattatt ggtaatttcg gcatacgtcc
     3541 agctttactt gtgtttgttc cgttatttac tttgtggttc atgttgtggg atgaaaagaa
     3601 gtaccgtcaa tctgagcggc gcagaattca tgaagatcat ttctatcatg agccctacta
     3661 cagatccagg caatcataat aaggattgac tgctaattat tcaaaagaca ag
//
LOCUS       DACEEZ010000156         3678 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20313_2017
            SAMN12639966-rid9879223.denovo.162, whole genome shotgun sequence.
ACCESSION   DACEEZ010000156 DACEEZ010000000
VERSION     DACEEZ010000156.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639966
            Sequence Read Archive: SRR10023158
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3678)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3678)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741683.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741683.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:44:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,016
            CDSs (total)                      :: 2,962
            Genes (coding)                    :: 2,889
            CDSs (with protein)               :: 2,889
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 36 of 73
            Pseudo Genes (incomplete)         :: 29 of 73
            Pseudo Genes (internal stop)      :: 18 of 73
            Pseudo Genes (multiple problems)  :: 8 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3678
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639966-rid9879223.denovo.162"
                     /isolate="E20313_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(150..1337)
                     /locus_tag="IWO89_002810"
     CDS             complement(150..1337)
                     /locus_tag="IWO89_002810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256-like element IS16 family transposase"
                     /protein_id="HAQ0457499.1"
                     /translation="MTQVHFTFESEEIQAIINESGANDTAKTLMTIMFNQLMEEQRNQ
                     YIQANAYERSEERQSQRNGYYDRSFTTRIGTLELHVPRTRDGKFSPTIFERYQRSEKA
                     LIAAMIEMVISGVSTRKVTKTVELLTDGATVSKSFVSNLMKQLDPFVFEWRNRSLEGS
                     EYPFFMCDALYMKVRENHRIVSKGVYIGIGIDSDGRRTILGFDVQDGESEDNWDTVFQ
                     SFVQRGLFGVKLVISDAHKGLVKAVRKNFLGASWQRCQAHFLRNIFDKLPKKVSSDVK
                     DELKSIFKASELELTRERKEHFLEKYGCDSKLSAACDILENGFEDAIQILSFPENIRR
                     RIRTTNVLERLNEEIRRRERVIRIFPNINSITRIIGTLLMEKDTEWLASPRKYLEFNS
                     NNI"
     gene            1447..1824
                     /locus_tag="IWO89_002811"
                     /pseudo
     CDS             1447..1824
                     /locus_tag="IWO89_002811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336725.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
     gene            1872..3272
                     /gene="yfcC"
                     /locus_tag="IWO89_002812"
     CDS             1872..3272
                     /gene="yfcC"
                     /locus_tag="IWO89_002812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative basic amino acid antiporter YfcC"
                     /protein_id="HAQ0457500.1"
                     /translation="METMKRKFTFPHSYTLIFILIILAAILTWIIPSGQFETVVENVA
                     GMEKTTVVPGTYHQIDKADYGQGFSSILMAPAEGIISAVEVVAFVLIVGGAFGIILKT
                     GAIDRGLFTLAKVLGDKGILVIPMSMILFSLGGSTFGMSEEVIPLFAVFISLMFSLGY
                     DSMTAILILFLSTQAGYVGSTINPFNVLIAQGVAGVYGNPLLWFRMLSWIVITGISIV
                     FTMLYASRVKKNPLSSIVYESDLKVKHKFQNFDTEKEFSTQDKLIIAGFIVGLGIMIW
                     GIISKGWYMVEISALFLGLGLFAGGIARFSQKEIAEAFVEGCSDFAYAAVIIGLARGV
                     LVILEGGMIIDTILNSLANLLGGLPKFLFTTILLISQVIVSFFVPSSSGAAALTMPVM
                     APLGDLMNVSRDVVVLSNQFGNGLMNIISPTSGVLLAGLAIAEINFSKWFKVGFKIFA
                     ILTLVSAVLLYIATLF"
     gene            3305..3472
                     /locus_tag="IWO89_002813"
     CDS             3305..3472
                     /locus_tag="IWO89_002813"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0457501.1"
                     /translation="MIKINQLAGMNIHYLFYSLKEFFAYQQKIGISSVELWGGAPHFF
                     IDAFTGSVAKF"
     gene            3526..>3678
                     /locus_tag="IWO89_002814"
     CDS             3526..>3678
                     /locus_tag="IWO89_002814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017370594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS6 family transposase"
                     /protein_id="HAQ0457502.1"
                     /translation="MNHFKGKQFQQDVIIVAVGYYLRYNLSYREVQEILYDRGINVSH
                     TTIYRWV"
BASE COUNT         1052 a          684 c          734 g         1208 t
ORIGIN      
        1 taaaagttca acctcatcaa aagcacaatg ttttgcgttg tgaacctaac tgccatgcct
       61 ttctttctga ggagagaggt caggagcagt agcgcagcga ctcttgatgt ctctcctcag
      121 aaagaagaga ctcgcacagg ttcatggtgc taaatgttat tcgaattaaa ttctaaatat
      181 tttcgtggag aagccagcca ctcagtgtct ttttccatca aaagtgttcc aattatccga
      241 gtgatggaat tgatatttgg aaaaatccga atcactcgtt ctctccggcg aatctcttcg
      301 ttcaaccgtt ccaacacatt ggttgtacga atccttcggc ggatattttc aggaaaagac
      361 aaaatttgaa tagcatcttc aaagccgttt tctaaaatgt cgcaagcagc acttaatttt
      421 gaatcacaac catatttttc caagaagtgc tcttttcttt cacgtgtcaa ttcaagttca
      481 gaggctttaa agatactctt caactcgtct tttacatcag aagaaacttt ctttggcagc
      541 ttatcaaaaa tatttctcaa aaagtgggct tggcatcttt gccagctcgc acctaaaaag
      601 ttcttgcgaa cagccttaac taaccctttg tgtgcatcag aaatgaccag ttttacaccg
      661 aacaaacctc gttgaacgaa cgattgaaaa acggtatccc aattatcttc cgattcgccg
      721 tcttgaacat caaaaccaag aatcgtacgt cgtccatcag aatcaatgcc gataccaata
      781 tatacacctt ttgagacaat acgatgattt tcccttactt tcatatacag ggcatcacac
      841 atgaaaaatg gatattccga gccttctagg ctccggtttc tccactcaaa aacaaatgga
      901 tccaactgtt tcatcagatt tgaaacgaat gatttagaaa cagtcgcacc atccgttagc
      961 agttcaactg ttttagttac tttgcgagtg gagacaccag aaatgaccat ttcaatcatg
     1021 gcagcgatca aagctttttc gcttcgctga tatctttcaa agatagtagg actaaactta
     1081 ccgtctctgg ttcgtggaac atgaagttct aacgtaccaa ttcgagttgt aaacgaacga
     1141 tcgtagtaac catttctttg tgattgccgt tcttctgaac gttcataggc atttgcctga
     1201 atatattgat ttcgttgttc ttccatcagc tgattaaaca tgatagtcat aagtgttttt
     1261 gcggtatcat tcgcaccgct ttcgtttata attgcttgaa tttcttcgct ttcaaaagta
     1321 aaatgtactt gagtcattga tatcagtcct attcgagtat gttttctgca aaaaacattg
     1381 tactcctaac tgagatcaat ggcttttttg atgcttcact tttacacaat tatatggacg
     1441 taatcaaaaa atgtttccac agaggacttc tttaattatt gtcgattaga ggaaagtggt
     1501 gtaacagtca ctagcggtac agatttaccg ctgtttatta ctgatattcc tgaatcgatt
     1561 ataagagcag tattccgtcg ttttcctgaa ggagaagaag tctgggagaa agaaacaagc
     1621 ttcacgacaa ccgagctgct gaaaagtttt acaagcaaca gcttcaaagt aaatggttta
     1681 tcggagtacg gagaaataaa agtagggaaa agcgccagct tagctgtttt cagcgaggac
     1741 ctctgtactg atgactggca aaaagtgatg gctgcggaag ttatcgctac ctatcttgat
     1801 ggtgacttag tttacaagaa ataatgcttt tcgatataac gttataaaac gaattaaaga
     1861 gaggttaaaa aatggaaaca atgaaaagaa aattcacctt cccacatagt tatacattga
     1921 tttttatctt aattattttg gctgcaattt taacttggat aattcctagt ggtcagtttg
     1981 agacagttgt tgaaaacgtc gcaggtatgg aaaaaacaac ggttgttccg ggaacctatc
     2041 accaaatcga taaggcagat tatggacaag gtttttcctc tatactaatg gcaccagctg
     2101 aagggattat ttcagctgtt gaggttgttg cttttgttct catcgttggt ggtgccttcg
     2161 ggattatcct aaaaacggga gccatcgatc gagggctgtt taccttagca aaagttctgg
     2221 gagacaaggg gatcttagtt attccgatgt cgatgattct attcagctta ggaggatcaa
     2281 cctttggcat gagcgaggaa gtcattccct tatttgcggt gtttatttct ctgatgtttt
     2341 ctcttggcta tgattccatg actgcgatct tgattctatt tttaagtaca caagcgggat
     2401 atgttgggtc gacgattaat cccttcaacg ttttaatcgc acagggagta gctggcgttt
     2461 acggaaatcc cctgctttgg tttcggatgc tatcctggat tgtcattact gggatttcaa
     2521 ttgttttcac catgctttat gctagcagag tcaaaaagaa tccactgtcc tctattgttt
     2581 atgagagtga tttaaaggtt aagcataagt ttcaaaattt tgacactgaa aaagagtttt
     2641 caacacaaga taaactaatt atcgccggtt ttatcgtagg tttaggaatc atgatttggg
     2701 gtatcatttc caagggatgg tatatggttg aaatttctgc actattctta ggattaggtc
     2761 tttttgctgg gggcatcgct agattttctc aaaaggaaat tgctgaagct tttgtcgaag
     2821 gctgttcaga tttcgcctat gctgctgtaa tcattgggct agctagagga gtcttggtaa
     2881 tcttggaagg tggaatgatt attgacacta ttctaaacag cttggctaac cttttaggtg
     2941 gtcttccaaa attcctgttc actacgattc tactcatttc tcaagtaatt gtttcctttt
     3001 ttgttccatc ctcctcagga gctgcggcat taacgatgcc ggttatggca ccattgggtg
     3061 atctaatgaa tgtaagtcgt gatgttgttg ttctaagtaa ccagtttgga aatggtttaa
     3121 tgaatattat ctccccaact agtggtgtat tactggcagg attagccatc gcagagatta
     3181 atttctctaa atggtttaag gttggcttca aaatatttgc catcttaacc cttgtttctg
     3241 ctgttttgtt atacattgct acactctttt agtcttaatc atccattaat aggaggcaat
     3301 agttatgatt aaaataaatc aattagcagg aatgaatatc cactatttat tttattctct
     3361 caaagaattt ttcgcttatc aacagaaaat tggtatttct tctgttgaac tttggggagg
     3421 tgctccgcat tttttcatag atgcttttac tggttctgtt gcaaagtttt aaatctacta
     3481 tcaaataagg tagaataata gaaaaagata gcaggaggaa tgacgatgaa tcattttaaa
     3541 ggaaagcaat ttcagcagga tgtgattatt gtagccgtgg gctactatct tcgttataac
     3601 cttagctatc gtgaagttca agaaatctta tatgatcgtg gcattaacgt ttctcatacg
     3661 acgatttatc gttgggtg
//
LOCUS       DACEEZ010000157         3199 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20313_2017
            SAMN12639966-rid9879223.denovo.164, whole genome shotgun sequence.
ACCESSION   DACEEZ010000157 DACEEZ010000000
VERSION     DACEEZ010000157.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639966
            Sequence Read Archive: SRR10023158
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3199)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3199)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741683.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741683.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:44:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,016
            CDSs (total)                      :: 2,962
            Genes (coding)                    :: 2,889
            CDSs (with protein)               :: 2,889
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 36 of 73
            Pseudo Genes (incomplete)         :: 29 of 73
            Pseudo Genes (internal stop)      :: 18 of 73
            Pseudo Genes (multiple problems)  :: 8 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3199
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639966-rid9879223.denovo.164"
                     /isolate="E20313_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            301..585
                     /locus_tag="IWO89_002815"
     CDS             301..585
                     /locus_tag="IWO89_002815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0457503.1"
                     /translation="MDILVRNIDPKFVATIDKRCQELTNKTGEKWSRNKYLKVLIESD
                     FDRQLTTYKKDQFDLLLDKFIAIQTENTQILSEYVYTNNQIIEMLIGGMI"
     gene            588..1004
                     /locus_tag="IWO89_002816"
     CDS             588..1004
                     /locus_tag="IWO89_002816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001232859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0457504.1"
                     /translation="MRKTSVQKHVTLYPSHVDVLDSIIEKNPDINNYSEAIRFLCMSY
                     EPDTNTTTTSNQAKLNAMGKEISIMTEIIAEFADLYLKDTGVLIGTNSKVYQEVKEKI
                     EAKLNPSNRNRPKNHQKETSQTVTSRNFDPTNFLKR"
     gene            complement(1141..1860)
                     /locus_tag="IWO89_002817"
     CDS             complement(1141..1860)
                     /locus_tag="IWO89_002817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HAQ0457505.1"
                     /translation="MDEKTELTVKYQNELNLVPLKNFNAKQMDLFFALCARMKDRGLK
                     KVRFSFEELKELSDYKMTATKAFVGDLEKLYKDMLNLTYRTEDEDSIRYFVLFTGFEI
                     NKTKKFVEVSVNPDLEYIINGLTAEFSRFELSAFTSLRSTYAKTLFRLLMQYRSTGYY
                     VVRIEEFRSLMDIPEYYQMGNIDQKVLNPALRELKKYFNDLKVTKIKAKKGNKIAKLE
                     FTFSSLTSDLPKISLRNWLEE"
     gene            2513..2608
                     /locus_tag="IWO89_002818"
     CDS             2513..2608
                     /locus_tag="IWO89_002818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000493114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Fst family toxin"
                     /protein_id="HAQ0457506.1"
                     /translation="MCFVFTNFICSLLVGLLSALFSYWLNNRNKK"
     gene            complement(2910..3155)
                     /locus_tag="IWO89_002819"
     CDS             complement(2910..3155)
                     /locus_tag="IWO89_002819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0457507.1"
                     /translation="MRLEKEKMEKFPYEYFQNLEKLEQAIQAIKFDLTDEKLLEYKNM
                     TPERKRSLEKLMQLVNEDGGISDAAFDAWSEFHGEYN"
BASE COUNT         1065 a          597 c          499 g         1038 t
ORIGIN      
        1 aaaaaaaatg tcgtttgaaa aactaaataa atttttgatc gtgcattttt aaatttggtt
       61 atcgattaca cgtgcagcac tcccccccga attttaaaga gcataaggaa acgtgtaccc
      121 actttctaaa aaaagtgggt agataggctg tgcctatcac gtttctcaga aaaaaatttg
      181 atatcacttt ttcgccatcg tgggtagctt atgtatagca ttttggcaaa aaacagctat
      241 acaaatatag tatttgtatg gtatacataa attatacata attgcaaaag gagatttttt
      301 atggacattt tagttcggaa tattgatcca aagtttgttg caacaataga caaacgttgc
      361 caagaactca cgaataaaac aggagaaaaa tggagtcgaa ataaatatct aaaagtttta
      421 atcgaaagtg attttgaccg tcaacttacg acttacaaaa aagaccaatt cgatttattg
      481 ctcgataaat ttattgctat ccaaacagaa aatactcaaa tattaagcga gtatgtctac
      541 acaaataatc aaatcattga aatgcttatc ggaggaatga tataaccatg agaaaaactt
      601 ctgtccaaaa acatgttact ttgtacccta gtcatgttga cgttttagat tccatcatcg
      661 aaaaaaatcc agatatcaat aactactcgg aagccattcg ctttttatgt atgagttatg
      721 aacccgatac caacacgacc actaccagca atcaggcaaa actaaatgct atgggaaaag
      781 aaatttccat catgactgaa atcatcgcag aatttgctga tttatatcta aaagatacag
      841 gcgttcttat tggcacaaat tctaaagtat atcaagaagt gaaagaaaaa atcgaagcta
      901 aactaaatcc atcaaatcga aatcgaccaa agaatcatca aaaagaaacc agtcaaacag
      961 tcacttctag aaatttcgac ccaacaaatt ttctaaaacg ataaaaaaca gataggagtt
     1021 attcctatct gttttttatt tagaagcccc atacaatatg ctagaatgta ctagcaatct
     1081 tagtcggttg taagggactt gtctacttgc ttaatgtaag gggggcgagt tcttttttat
     1141 ttattcttct aaccagttac gcagagaaat ctttggcaaa tctgacgtta ggcttgaaaa
     1201 agtaaattct aatttggcga ttttattgcc cttttttgct tttatttttg tgactttaag
     1261 atcattgaag tattttttta gttctcgtaa tgcaggattt aatacttttt gatcaatgtt
     1321 acccatttga tagtattctg ggatatccat taagctccta aactcttcaa tacgaactac
     1381 ataataacca gtagaacggt actgcataag cagtcggaat agagtttttg catacgtaga
     1441 acgcaacgac gtgaaagcag ataattcaaa tctactaaat tcagctgtta aaccattaat
     1501 aatatattct aggtccggat taacactgac ttcaacaaac ttttttgttt tattgatttc
     1561 aaaaccagta aacaaaacaa agtagcgaat agaatcctca tcttcagtac ggtaagtaag
     1621 attaagcata tctttataga gcttttctaa atcaccgaca aaggcttttg tagcagtcat
     1681 cttataatca cttaactctt ttaattcctc gaaagaaaat ctaacttttt tcaaaccacg
     1741 atctttcata cgagcgcata atgcaaaaaa taaatccatc tgcttagcat tgaaattttt
     1801 cagcggtact aaattcaatt cattttgata tttgactgtt aattccgttt tttcatccat
     1861 tcctttcttc cccccttaca aataaataat aacctttaac aaccaactcg taaataggtt
     1921 ggttgttatt atacaaaagg atattagtac tcagataaaa agttgttagt actcagataa
     1981 aaagttgtta gtactcagat aaaaagttgt tagtactcag ataaaaagtt gttagtaccc
     2041 ttctgtatcc gttgggagag taaggctcaa ctgcccccta aaaagggttc taaaaaatct
     2101 ttaaaaaata aataaaataa acgcgcgcgc gcgagcaaaa tttcccgcca aaaaatcacc
     2161 cactttttaa aataaacagg ttcgcgcatg atattaaact gccaatctag ctccctatgg
     2221 tcgctgtaag aaggagtgat cgctacgctc tcacatttta cttgcagcag tcgcctttag
     2281 gctccttcgc caaaaataat tcattaggga ctcctttgtg gcgtcctgag agctcgtcta
     2341 agccgttcta agacaagatt cgataaaaac tatgtccttc gttctaaagc gccttacagg
     2401 cggtcagggc aagccctaac aaccccaagg caataaaaaa cttaggctta tttttgcttt
     2461 tttattgtat aattgattgt cgcctatccg acagtcttgc gactggaagg aggtgtgttt
     2521 cgtgttcact aactttattt gctcccttct tgttgggctt ttaagcgctt tgttcagtta
     2581 ctggttgaat aaccgaaaca agaagtaaaa cttaattaag gcgactcagc ccacaaaaaa
     2641 agccccagag aatccgacct ctctggggct ttcgtgtgtt tcgtgttcac ttatttgcaa
     2701 tcaaattata acagaaataa atatttattt caaaaatatc ctacaaaagt ctaaaatccg
     2761 taattatttc ttatatgcaa catttacaaa taaagtcgct ctgagagctc gtatttttcg
     2821 atttaagacg ttttatttag tttttggtgt atttcattgg tccaagctaa aaaactctta
     2881 gaagcgattt tagagccatt taaacccact taattatatt ctccatgaaa ttcgctccat
     2941 gcgtcaaaag ctgcgtctga aattccgccg tcttcgttca ctagttgcat taatttttcc
     3001 aacgatcgtt ttctttcggg tgtcatattt ttatattcca acaatttttc gtctgttaaa
     3061 tcgaacttga ttgcttgaat tgcttgctct aatttttcta agttttgaaa atattcgtaa
     3121 ggaaattttt ccattttttc tttctccagt ctcacaattt ttctagaaat aaaatcactt
     3181 ctttgccgtc tatactctc
//
LOCUS       DACEEZ010000158         3024 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20313_2017
            SAMN12639966-rid9879223.denovo.165, whole genome shotgun sequence.
ACCESSION   DACEEZ010000158 DACEEZ010000000
VERSION     DACEEZ010000158.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639966
            Sequence Read Archive: SRR10023158
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3024)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3024)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741683.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741683.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:44:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,016
            CDSs (total)                      :: 2,962
            Genes (coding)                    :: 2,889
            CDSs (with protein)               :: 2,889
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 36 of 73
            Pseudo Genes (incomplete)         :: 29 of 73
            Pseudo Genes (internal stop)      :: 18 of 73
            Pseudo Genes (multiple problems)  :: 8 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3024
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639966-rid9879223.denovo.165"
                     /isolate="E20313_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            <1..1243
                     /locus_tag="IWO89_002820"
     CDS             <1..1243
                     /locus_tag="IWO89_002820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010729806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAQ0457508.1"
                     /translation="KTKDQLNEFFANVLETPYWYAKKILSLLSECLDQPVYEEDIFFI
                     TLYFYSLNVTNVKTGIKSIVLAHGYSTASSMANVANRMLNKNVFQAYDMPLDITLEKI
                     EAKLIQFLGDYRTDSGLILLVDMGSLNQLGGLLVNHLNGPLLIIDHINTPLLLEVGQQ
                     VLNNKSITEIGAEIETSLTFKKQLLLPKKKKKKAIITCCYTGIGSATQIQEILTKCLK
                     DAVRELVIIPYDYKKLLENKNFETPFQLYDVIMIVGTEDPKINQIPYIGLDRLINGGS
                     IGEFVHYLEKELTIEVNDLQKELIFNFSISKIVENLTILDPDKVLSFVQKAIKELSKL
                     LNFDFSNNQLFLLYIHCSCMIERIVRKESVDEQEDIEEYMQANGEIMDKIHSAFFEIE
                     REYTIEIPDLELRLLSDIVQG"
     gene            1256..1888
                     /locus_tag="IWO89_002821"
     CDS             1256..1888
                     /locus_tag="IWO89_002821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010729807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HAQ0457509.1"
                     /translation="MTMGVIFDMDGVLIDSETFYFERRMNFFQELEIDPGSNQIDDYV
                     GKTEAAIWETLVPEDKSLREELYPKYVDYRKTHPIDFAKALRKEVKPLLDFLKKRDIP
                     LALASSSPAVEIESMLIQCELTEYFSFVISGESLKQSKPHPEIYLKAKQQLDCDFFIA
                     VEDSTIGIKSAKAANIYTAALKQPMQIDQSEADIQIESLQELEQIVLEKG"
     gene            2093..2320
                     /locus_tag="IWO89_002822"
     CDS             2093..2320
                     /locus_tag="IWO89_002822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009320234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-ribosylglycohydrolase family protein"
                     /protein_id="HAQ0457510.1"
                     /translation="MLQQNQNTNSYQAAILTAVNLGGDTDTTAAVTGALAGILYGGEV
                     IPENWRKSLQNDQLLKTISQRFCDTVLSQRI"
     gene            2389..2655
                     /locus_tag="IWO89_002823"
     CDS             2389..2655
                     /locus_tag="IWO89_002823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002383791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin"
                     /protein_id="HAQ0457511.1"
                     /translation="MLVQMMNGGRIMSQSTITFRIPDDEKELVLEYAKAHNSSLTELY
                     RNAVLDKIEDEIDLRTLRSAIEVSKEKKESGISQEEMEKLLNEL"
     gene            2645..2914
                     /locus_tag="IWO89_002824"
     CDS             2645..2914
                     /locus_tag="IWO89_002824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HAQ0457512.1"
                     /translation="MNYKVIYMPQAQKQLKKMDRNQAKIIVAWIRKNLENTTDPRQHG
                     KGLTGNKSGEWRYRIGNYRILANICDSEIEIQIFSIGHRSTIYKR"
BASE COUNT         1072 a          443 c          607 g          902 t
ORIGIN      
        1 gaaaaccaaa gatcagctta acgaattttt tgctaatgtt cttgaaaccc cttattggta
       61 tgcaaaaaaa atcttaagcc ttttatctga gtgtttagat caacctgtgt atgaagagga
      121 cattttcttt attacgctat atttttatag tttgaatgtc acgaatgtta aaacaggtat
      181 taagagtatt gtgctagctc atggttattc tacagctagt agtatggcga atgttgccaa
      241 ccgtatgctg aataagaacg tttttcaagc ttatgatatg cctttagata ttacattaga
      301 gaaaattgaa gcaaaattga ttcaattcct tggagattat cgaacagatt caggcttgat
      361 tttattggta gatatgggtt cactaaacca actaggcggt ttattagtga atcatctaaa
      421 tggaccactg ttaatcattg atcatatcaa tacaccgtta ttattggaag tcggccaaca
      481 agtattaaac aataaaagta tcaccgaaat aggagcagaa attgaaacaa gtcttacttt
      541 caaaaagcag ttactacttc ccaagaaaaa gaaaaagaaa gccatcatta cttgttgtta
      601 tacagggatt ggtagtgcga cccagattca ggagatttta acgaaatgct tgaaagatgc
      661 agttagagaa ttagtaatta ttccgtatga ttacaaaaag ctgttagaaa acaagaattt
      721 tgagactcct tttcaattat acgatgtcat catgattgta ggtacagaag atcccaaaat
      781 taatcagatt ccatatattg gattagatcg cctgattaat gggggaagca ttggcgaatt
      841 tgttcattac ttggaaaaag agctgactat tgaagtgaac gatttacaaa aagaattaat
      901 tttcaatttt tcaattagta aaattgtcga aaatttaacc attttggatc cagataaggt
      961 gctatctttt gtacaaaaag caattaaaga attatcgaag ttattaaatt ttgatttttc
     1021 taataatcag ctgtttttgt tgtacattca ctgtagttgt atgattgaac gaattgtacg
     1081 taaagaaagt gtggatgagc aagaggacat tgaagaatat atgcaagcaa atggagaaat
     1141 aatggataaa attcatagcg ctttttttga gattgaaagg gaatacacaa ttgaaatacc
     1201 agatttagag ttgcgattat tgagcgatat tgttcaagga taggagtgaa ttcgaatgac
     1261 aatgggagta atttttgata tggatggggt actcatagat agcgagacat tttattttga
     1321 gagaagaatg aacttctttc aagaactgga gatagatcca ggatcaaacc agattgatga
     1381 ttatgtgggg aaaacagaag ctgcaatatg ggaaacatta gttcctgagg acaaatcatt
     1441 aagagaggaa ctttatccga aatatgtaga ttatcgaaaa acgcatccaa ttgatttcgc
     1501 taaagcttta cgaaaagaag tgaaaccgtt acttgacttc ctcaagaagc gggatatacc
     1561 attggcactg gcatcttctt ctccagctgt cgagatagag agtatgctca ttcaatgtga
     1621 acttactgaa tatttttcct ttgttataag tggtgagtca ttaaaacaaa gcaaaccaca
     1681 tcccgaaata tatttaaaag cgaaacagca attagactgt gacttcttta ttgcagtaga
     1741 ggattcaacg attggtatta aatcagcaaa ggcagcaaat atctatacgg cggcactaaa
     1801 acaaccaatg caaatcgatc agagtgaagc agacatacaa attgaaagtc tacaagaact
     1861 ggaacaaatt gttttagaaa aaggttagtg aattaatgga aataaggcta tatatctgtg
     1921 ctagctattt tgactaacca aaaaaacttg ctataattca ggtgtagtaa aaggtgctgg
     1981 taacaccttt tacgaaaatg cttcggcagt gataatggga ttggcttttt aagtttttta
     2041 ttttgttcgc aatccctaag gtcatgtgaa tgatcctagg gattgctaga atattttgca
     2101 acagaaccaa aatacgaata gttatcaagc agccatattg actgcggtca atttaggagg
     2161 ggatactgat actactgctg ccgtaactgg tgctcttgcg ggtattcttt acgggggtga
     2221 agtgatccct gaaaattgga gaaaatcgct tcaaaatgac cagcttctta aaacgatcag
     2281 tcagagattt tgcgataccg tgctatctca aagaatttga ataatggata tgatgcaaat
     2341 cttgtttgta ctaacgttgt attgacaaaa aggattttat ttagtacaat gttagtacaa
     2401 atgatgaacg gaggtagaat catgtctcaa tcaaccatta catttaggat tcccgacgat
     2461 gaaaaagaac tggtattaga atatgcaaaa gcacacaatt cttcattgac cgaactttat
     2521 cgaaatgctg tactggataa aatcgaagat gaaatcgatc tacggacttt acggagtgca
     2581 attgaagttt ccaaagagaa aaaagagagt ggtatttctc aagaggaaat ggagaaactg
     2641 ctaaatgaat tataaagtca tctacatgcc gcaagcccag aagcaattaa aaaaaatgga
     2701 cagaaaccaa gctaaaataa ttgtcgcctg gatcagaaag aatttagaaa acacgactga
     2761 cccgcgtcag cacggaaaag gtttgactgg aaataagagt ggggaatggc gctatcgaat
     2821 tggaaactat cgaatattgg ctaatatttg tgattctgaa attgaaatcc agatattctc
     2881 aattggacat agaagtacaa tatataaaag ataaccgttt ttggaacgaa gtagagtttc
     2941 gaaagtcaat gtataatctt ttgaattttg cataatacca caggtagaaa ctacccgata
     3001 ctatcggttg gtttctgctt tttt
//
LOCUS       DACEEZ010000159         2992 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20313_2017
            SAMN12639966-rid9879223.denovo.166, whole genome shotgun sequence.
ACCESSION   DACEEZ010000159 DACEEZ010000000
VERSION     DACEEZ010000159.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639966
            Sequence Read Archive: SRR10023158
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2992)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2992)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741683.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741683.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:44:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,016
            CDSs (total)                      :: 2,962
            Genes (coding)                    :: 2,889
            CDSs (with protein)               :: 2,889
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 36 of 73
            Pseudo Genes (incomplete)         :: 29 of 73
            Pseudo Genes (internal stop)      :: 18 of 73
            Pseudo Genes (multiple problems)  :: 8 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2992
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639966-rid9879223.denovo.166"
                     /isolate="E20313_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(106..1053)
                     /locus_tag="IWO89_002825"
     CDS             complement(106..1053)
                     /locus_tag="IWO89_002825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HAQ0457513.1"
                     /translation="MKVSIVVPCFNEEKTVPLFFDEVEKFKGAHEFEYLFIDDGSKDG
                     TLAAIKKLASAYPYVRFVSFSRNFGKEAALYAGLQASTGDLVTVMDVDLQDPPELLPE
                     MINRITTSDIDCVGTRRSTREGEPAIRSFFAKKFYQLINKISDTEMVDGARDFRLMTR
                     QMVDAVLELTEYNRFSKGLFSWVGFKTEYISYENRERVAGETSWSFWKLFNYSIDGIV
                     NFSDAPLTIASFIGALSCVGSGIAILFIILRALLFGDPTSGWPSMVSIFLFIGGIQLL
                     CLGIIGKYIGKIFLETKQRPVYIIRETEKVKPNELKPHQ"
     gene            1299..1763
                     /locus_tag="IWO89_002826"
     CDS             1299..1763
                     /locus_tag="IWO89_002826"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HAQ0457514.1"
                     /translation="MKIHTPIGFLWLDADESGLTAVSFQPISESESANQRILTRTQEQ
                     FEEYFAGKRKEFDVPLSIKRGTPFQQNVWKALSTIPYGEIRTYKEVAVAVDSPKAVRA
                     IGQANRTNPLPIVIPCHRVIGQNGKLTGYMGNAIDGVEIKRQLLLLEGYLQN"
     gene            complement(1825..2868)
                     /locus_tag="IWO89_002827"
     CDS             complement(1825..2868)
                     /locus_tag="IWO89_002827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative sulfate exporter family transporter"
                     /protein_id="HAQ0457515.1"
                     /translation="MKSLSHPIKSVKDSAVREILPGLLLSFAIAAFSRLLAIYLPQLG
                     GATIAILLGILLGNTFFKQPVLAIGTKFAESKLLEFSVFLLGATVTAQTIAQIGVKGI
                     VFVLLQMSVTIIGAYLIGKKLQFSDTSSLMMAGGNAVCGSSAIASIAPSIHAKEEEKG
                     QIITLVNLLGTVMMLTLPFLGLQLFGGNLIEKSALLGGTLQSVGQVVAGASLLDNSVV
                     EFAMLFKILRIMMLVVVVYLFEKFMHKKQPALFETPTDTQKKRKLPLPWYVLGFVIFC
                     LVNSLFSLPVVIDQSAHFISSWFEITALAAIGLRLDFAKFMKEGTRFLSYGLAVGLLQ
                     TVMALLFIWLIKV"
BASE COUNT          989 a          593 c          571 g          839 t
ORIGIN      
        1 agaagctact tctggaacac catttattcg gaaaaaagga ctgtgacaag acttttgtca
       61 cagtcctttt tcatttaaaa aaatgtttac ttcatttgac gtatattatt ggtgaggctt
      121 caattcattt ggcttcactt tttctgtttc tcttatgata taaacaggac gctgtttcgt
      181 ttctaagaag attttcccaa tatatttccc gatgattcca agacataaca attggattcc
      241 accaatgaac aggaagatcg agaccatgga aggccatcct gatgtaggat caccaaacaa
      301 caatgcccgc aaaataatga acaaaattgc aataccagaa ccgacacaag acaaagcgcc
      361 gataaatgaa gcaatcgtta acggtgcatc cgagaagttc acgataccat caatcgagta
      421 gttgaataat ttccagaagg accaagatgt ttcgccagcc acacgttcgc ggttttcata
      481 agaaatatat tctgttttaa atccgaccca gctaaataat ccttttgaaa atcggttgta
      541 ttctgttaat tctaggacgg catccaccat ttgacgagtc atcaagcgga aatcacgagc
      601 gccatcgacc atttctgtat cgctgatttt attgatcaac tggtagaatt ttttcgcaaa
      661 gaaactgcgt atcgcaggct cgccttcacg agtcgaacgc cgggtgccga cacaatcgat
      721 atcagaagta gtgatccggt tgatcatctc aggaagcagt tcgggcggat cttgcagatc
      781 aacatccatc acggtcacta aatctcctgt agaagcttgc aatccagcgt acagagccgc
      841 ttctttccca aaatttctgg aaaatgagac aaagcggaca taaggataag ctgaggctag
      901 cttcttgatt gctgctaacg taccgtcttt tgatccgtcg tcaataaaca aatattcgaa
      961 ctcatgggca cctttgaatt tttcaacctc atcaaaaaaa agaggaacag ttttttcttc
     1021 attaaaacat gggacaacaa ttgatacttt catcgtttct atcctttcaa tcgtatgttt
     1081 atatatggtt tacattcaaa atttaataaa ccttaaataa cacctttaaa atttgatttt
     1141 acgtaaaaga acaggtattc tttttgtaaa ctaacgtatt tagtttacca tgtttcctcg
     1201 gtttatcaaa cttttaagaa cattttttat tagttggcaa gtagttttgg atagagaaaa
     1261 aggaggaagt tccagtctat tgactaggag caaacaaaat gaaaatacac acacctatcg
     1321 gctttttatg gttagatgca gatgaatccg gattgactgc tgtatctttt cagccaattt
     1381 cagaaagcga atcagcgaat cagcgaattt taacgcgcac acaagaacag tttgaagaat
     1441 attttgcggg aaaaagaaaa gaatttgatg ttcctttatc tataaagaga ggtacgcctt
     1501 ttcagcaaaa tgtctggaaa gctttgtcga ccattcctta tggagaaatc cgaacgtata
     1561 aagaagtagc cgttgctgtt gatagtccga aagctgtgcg agcgattggg caggcaaatc
     1621 gaaccaatcc attgccaatc gttattcctt gtcatcgagt gatcgggcaa aatggaaaac
     1681 tgacaggata tatgggaaac gcaatagatg gggttgaaat caaacgccaa ttgttgttgt
     1741 tagaaggata tctgcaaaat taaaaacagt tgtgattcgg acattgttcc atgcgagaat
     1801 cacaactgtt ttttcatgtt cattctaaac tttgatcagc caaataaata gtaaagccat
     1861 cactgtttgg agaagcccga cagctaatcc ataagataag aagcgtgtac cttctttcat
     1921 gaattttgca aagtctaaac gcagaccaat tgccgctaaa gcagtgatct caaaccagct
     1981 gctgataaaa tgtgccgatt ggtcaataac tacaggtaaa gaaaacaagc tgttgaccaa
     2041 acaaaagatc acaaacccta atacatacca aggaagcggt aattttcgtt ttttctgcgt
     2101 atcagttggc gtttcaaaca gagctggttg tttcttgtgc atgaattttt caaacagata
     2161 gacaacaaca acgagcatca taatacgtag aatcttaaat agcatggcga attcgacaac
     2221 agaattgtca agtaagcttg ccccagcgac tacttgacca acagattgga gtgtaccacc
     2281 caaaagtgca cttttttcaa tgagatttcc accaaacaac tgtaatccaa gaaaagggag
     2341 agtcaacatc atcacagtac ccagtagatt gactagtgtg atgatctgtc ctttttcttc
     2401 ctctttcgca tggatggaag gggcgattga agcaatagca gaagaaccac aaacagcgtt
     2461 tcctccagcc atcatcaaag aagaagtatc gctgaattgc agttttttac caatcaagta
     2521 agcaccgatg atagtgactg acatttgcag taacacaaaa acaattcctt ttacaccaat
     2581 ttgagcaatc gtttgagctg tgacagttgc acctagtaaa aaaacagaga attctaatag
     2641 tttgctttca gcaaattttg tgccgattgc tagaacaggc tgtttaaaaa aagtatttcc
     2701 taataaaata cccaataaaa tagcaatagt cgctccacct aactgtggca gataaatagc
     2761 aagaagcctg ctgaaggcag cgatggcaaa agaaagcagt aaaccgggta agatttcgcg
     2821 gaccgctgaa tcctttacag attttattgg atgtgacaat gatttcattt gatcggttcc
     2881 tttcgtcaat aaatactaaa agcatactcg aacatagtag cactaaacca gtattttgac
     2941 aaaagaacaa gaggtcgtga caaaagtctt gtacgacctc ttgtttcgaa ta
//
LOCUS       DACEEZ010000160         2861 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20313_2017
            SAMN12639966-rid9879223.denovo.167, whole genome shotgun sequence.
ACCESSION   DACEEZ010000160 DACEEZ010000000
VERSION     DACEEZ010000160.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639966
            Sequence Read Archive: SRR10023158
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2861)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2861)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741683.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741683.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:44:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,016
            CDSs (total)                      :: 2,962
            Genes (coding)                    :: 2,889
            CDSs (with protein)               :: 2,889
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 36 of 73
            Pseudo Genes (incomplete)         :: 29 of 73
            Pseudo Genes (internal stop)      :: 18 of 73
            Pseudo Genes (multiple problems)  :: 8 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2861
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639966-rid9879223.denovo.167"
                     /isolate="E20313_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(44..1363)
                     /locus_tag="IWO89_002828"
     CDS             complement(44..1363)
                     /locus_tag="IWO89_002828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008392738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HAQ0457516.1"
                     /translation="MKKLKIFPKMFIQIFSVLGIIIILVHSLIFFIFPKTYLETRKEK
                     IHNMANEISSNMNGKEIKYIEQTLEFYSKSSEIKVFIKEKNNKNEIQIKDNINVNLES
                     DSNSLIIEEREIKLNDGKKTNLQFVSTADMQKDAKDLSLKFLPYSLLISILFSAIISL
                     IYAKSIKNNIQEIKIVTDKMMKLDKKMSLKVSSNDEVGELKQQINDLYSTLLRTIDDL
                     EFKNKEILKLEKLKYDFFKGASHELKTPLASLKIILENMKYNIGKYKERDIYINECIE
                     IVDSLTKNISQILSVHSIENLNNDEEYLKINDTLEDILKKYEILANQKKITVNNYILD
                     ENVYIGKTALKIILSNLISNAVKYTDVNGVINIGIVNDWLYIENSYGNNKISNMDKIF
                     DVKFDLNKENSNGLGLYIVSNILNNYNIEYKVLQDEEFFIFKIKIFS"
     gene            complement(1360..2013)
                     /locus_tag="IWO89_002829"
     CDS             complement(1360..2013)
                     /locus_tag="IWO89_002829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAQ0457517.1"
                     /translation="MKILTVEDDNMIREGISEYLSEFGYTVIQAKDGREALSKFNSDI
                     NLVILDIQIPFINGLEVLKEIRKKSNLPILILTAFSDEEYKIDAFTNLVDGYVEKPFS
                     LPVLKARIDSLIKKNYGHLEKFEYKDLSVNFNSYTAKINGEEIDVNAKELEVLKCLLD
                     NNGQVLTRMQIIDYVWKDSEETPYDRVIDVYIKELRKKLQLDCITTIRNVGYKLERK"
     gene            2301..>2861
                     /locus_tag="IWO89_002830"
     CDS             2301..>2861
                     /locus_tag="IWO89_002830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HAQ0457518.1"
                     /translation="MDNHTRKLLGLTDKHLFFDEEWLIEKEYKGVQAQFIKGRLDDSP
                     SHCEHCGSIRIIRNGSYTTRTQILKVKEKLTILELKRTRFLCYDCGQTFSAKTDLVDE
                     HHQLTKELKQAILIELYENQSRKLIAKKYFVSDGTVTRILREATKHYQPRMNFLPTVL
                     CMDEFKSMKSVSGSMSFICVDGTTHQL"
BASE COUNT          938 a          460 c          315 g         1148 t
ORIGIN      
        1 taagtctcta tttttcaata actattgcaa aattatatct taattaagaa aatattttta
       61 ttttaaagat aaaaaattct tcatcctgca atactttata ttctatattg taattattca
      121 gaatattact tacaatatat aaacctaatc cattactatt ttccttattt aaatcaaatt
      181 taacatcaaa tattttatcc atattcgaaa ttttattgtt accgtatgaa ttctctatat
      241 ataaccaatc attaactatc ccaatattaa ttaccccatt tacatcagta tattttaccg
      301 cattactaat caaattagat agaataatct ttaaagctgt ttttcctata taaacattct
      361 cgtctaatat ataattattg acagttattt tcttttgatt tgctaatatt tcatactttt
      421 ttaatatgtc ttctaatgta tcatttattt tcaaatattc ttcatcatta ttcaaatttt
      481 ctatagaatg aactgataat atttgagaaa tattttttgt taaactatca acgatttcaa
      541 tacattcatt aatataaata tctctttctt tatattttcc aatattgtat ttcatgtttt
      601 ccagtattat tttaagacta gcaagagggg ttttaagttc atgggatgca cctttaaaaa
      661 agtcatattt taacttttct aattttaaaa tttctttatt tttaaattcc aaatcgtcaa
      721 ttgttcttaa taaagtagaa tacaaatcat taatttgttg ttttaattct ccaacttcat
      781 cattagagct aacttttaaa ctcatttttt tatcaagttt catcatttta tcagtaacaa
      841 tttttatttc ttgtatatta ttttttattg attttgcata aattaaagaa ataatagcag
      901 aaaataaaat tgatattaat aaagaatatg gtaaaaattt aaggcttaaa tctttcgcat
      961 ctttttgcat atcagctgta gaaacaaatt gtagatttgt ttttttacca tcattaagct
     1021 ttatttctct ttcttcaatt atcaaagaat tactatcact ttctaaatta acatttatat
     1081 tatctttgat ttgtatttca ttcttattat ttttttcttt tatgaatact tttatttcac
     1141 tactcttgga ataaaactcc aaagtttgtt ctatatattt tatttctttt ccattcatat
     1201 tactagaaat ttcatttgcc atattatgaa tcttttcttt tctcgtctcc aaatatgttt
     1261 taggaaagat aaaaaaaatt aatgaatgaa ctaatataat tattattcca agaacagaaa
     1321 atatttgtat gaacattttt ggaaatatct ttaatttttt cattttctct ccaatttata
     1381 tcctacattt cttatagtag ttatacagtc taattgtaat ttttttctaa gttcttttat
     1441 atatacatct attactcgat cataaggagt ttcttcgcta tctttccata cataatcaat
     1501 aatttgcatt cttgttaaca cttgtccatt attatccaat aaacatttta atacttctag
     1561 ttcttttgca tttacatcta tttcttcacc atttatttta gctgtatagc tattaaaatt
     1621 aaccgataga tccttatatt caaacttctc taaatgtcca taattcttct taattaaaga
     1681 atctattcta gcttttaaga ccggcaatga aaatggcttt tctacatatc cgtctactaa
     1741 attagtaaat gcatcaattt tatattcttc atcactaaat gcagtcaaga ttagaattgg
     1801 taaattgctt ttctttctaa tttctttcaa cacttctaaa ccatttataa aaggtatctg
     1861 aatgtctaag ataaccaaat ttatatcgct attaaatttt gacaaggctt ctcttccatc
     1921 tttagcttga ataacagtat atccaaattc tgaaagatat tcacttattc cctctcttat
     1981 catattatca tcttcaacag ttaatatttt catatatacc tccacttaag cctatacact
     2041 agatattata cagcatatta tggctctata atatctagtg ttggtgacac cttctgttgc
     2101 caatactttt tcttttttct aataaatgca aacagacacc ttgaaatcct ttagaataaa
     2161 gggttctata atattttgtg ttgttggaaa atcgaaggcg attttctact aacacttttt
     2221 cttcgtttca actcaaaaag agactcactt ttggtaaaat ttagtcgacc aaaacatcac
     2281 cctaccaaag gagtcgctct atggataatc atactagaaa attactcgga ttaacagata
     2341 aacatttatt ttttgatgaa gaatggctga ttgaaaaaga atataaaggc gttcaagcgc
     2401 agtttattaa agggagattg gatgactcac cttcccattg tgaacactgt ggatcgattc
     2461 gtattatccg caacggttcc tatacgacac gtactcagat attgaaagtc aaagaaaagt
     2521 tgactatttt agaattaaaa cgtacccgtt ttctatgcta tgattgtggg cagacctttt
     2581 ctgcgaaaac ggacttagtt gatgaacatc atcaactaac aaaggaactg aagcaggcga
     2641 ttctcataga actgtacgaa aatcaatcac gtaaactaat cgctaagaag tacttcgtct
     2701 cggatgggac ggtgacacgg atcttgcgtg aagcaacgaa gcattaccag ccacgtatga
     2761 acttccttcc gacggtgttg tgtatggatg agttcaaatc aatgaagtct gtttctggtt
     2821 ccatgagttt tatttgtgtt gatgggacaa cccatcagct a
//
LOCUS       DACEEZ010000161         2844 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20313_2017
            SAMN12639966-rid9879223.denovo.168, whole genome shotgun sequence.
ACCESSION   DACEEZ010000161 DACEEZ010000000
VERSION     DACEEZ010000161.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639966
            Sequence Read Archive: SRR10023158
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2844)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2844)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741683.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741683.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:44:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,016
            CDSs (total)                      :: 2,962
            Genes (coding)                    :: 2,889
            CDSs (with protein)               :: 2,889
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 36 of 73
            Pseudo Genes (incomplete)         :: 29 of 73
            Pseudo Genes (internal stop)      :: 18 of 73
            Pseudo Genes (multiple problems)  :: 8 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2844
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639966-rid9879223.denovo.168"
                     /isolate="E20313_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            144..494
                     /locus_tag="IWO89_002831"
     CDS             144..494
                     /locus_tag="IWO89_002831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAQ0457519.1"
                     /translation="MSRLIIQTAFYKAIIPENLDEVKLYLWEDNGEIVGFSGVSAEEL
                     VMLFLDPDFIGKRYGSQILIELIETEDIKRIDVNSQNKYAKHFYLSHGFEIESEEDLD
                     GFGKPYPITHLVKK"
     gene            908..1726
                     /locus_tag="IWO89_002832"
     CDS             908..1726
                     /locus_tag="IWO89_002832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HAQ0457520.1"
                     /translation="MNKNPHNLKLVAVDMDGTFARTDYTYDIPRFKAVLSRMKAVDCQ
                     FVVASGNQYYQLRSLFPNYYDELSFVAENGAFVKDKTEVLFSADMPRKAIEVVLDLCK
                     DYPEVKNVMCGLNSAYCERGKVSQAFFDLTNIYYHRLEWVADFAEVQDKILKFAPTMP
                     VEKTEFYVELFREKLAGLLVPTSSGHGSVDLILSECHKTFGLKMLVERLGINPDQCVA
                     FGDGGNDIEMLEYCGLSYEMDNATEAVKQVAKHQCPSNDEDGVLVTLDRLFPNT"
     gene            complement(1799..2158)
                     /locus_tag="IWO89_002833"
     CDS             complement(1799..2158)
                     /locus_tag="IWO89_002833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster biosynthesis family protein"
                     /protein_id="HAQ0457521.1"
                     /translation="MSDNLFLEVQPEVFKQVPRLKDGFLLTLNDGSNQFSSAEGCCMI
                     GDHFLLVSTQHYIPAYDLTIPCEEATVKTSEYDTTFINGHLVLEVTPSNQTIRLKNES
                     GILDSNVQVYFDSSDAS"
BASE COUNT          969 a          399 c          505 g          971 t
ORIGIN      
        1 gcaaccggag tttcttgatg aaaatatgct tctatcatta caagctcgtt tggtgtaaga
       61 tgggtataag tcatttatgt tcactctcct tgtatgcttt agcgggtatt acaatttgag
      121 tgtaacataa atggcttttt tatttgtctc gcttaattat acaaacggcg ttttataaag
      181 caatcattcc tgagaactta gatgaggtta aactttattt atgggaagat aacggagaaa
      241 ttgtcggttt tagtggtgta agtgcagaag aattagttat gctatttctt gatccagatt
      301 ttattggtaa gcgatatggt agtcaaatat taattgagct gattgaaact gaagatataa
      361 aaagaattga tgttaatagt caaaataaat atgcaaaaca tttttattta agccatggat
      421 ttgaaattga atctgaagaa gacttagatg gttttggtaa accataccct ataacgcatt
      481 tagtaaaaaa ataattcatg aatggtttat tacctgattg aacatgaata aaagtaatat
      541 ttttattttg atggcctatt tctaggttat gaattatcca gatcaaattt gtgtattaca
      601 ttcagtaata aggtgcttat cttaggatca acttatgata ctagctgacc aaggatgtac
      661 cagttataga aaatcagtta aagtaaaaat gataaaaatc ttatgtcaac actgatttag
      721 gctgacctgt gggttaggag aaagacaggt cggtagcttt taatacaaaa aatggcttgt
      781 atgtatctag atagttaact gaaattagaa atgttcttat ttttaacgga gtaagaaaaa
      841 taggctgctt taggtctgaa tactttttca atataatgaa gatatcaaag gcgacaggag
      901 tgtttttatg aataagaatc ctcataatct aaagttggta gcagtagata tggatggaac
      961 atttgctcgt actgattaca catacgatat cccaaggttc aaggctgttt tatcgcgaat
     1021 gaaagctgtt gattgtcaat ttgttgttgc aagtggaaat caatactacc aattgagatc
     1081 gttatttcca aattattatg atgaattaag ttttgttgct gaaaatggtg cttttgttaa
     1141 agataaaaca gaggtactat tttcagccga tatgcctaga aaagcgatcg aggtggtcct
     1201 tgatttatgt aaagactatc ctgaagtaaa aaatgtgatg tgtggtctga atagtgccta
     1261 ctgtgaaaga ggaaaggtga gtcaagcatt ttttgatcta accaatatct attaccatag
     1321 attagaatgg gtagctgatt ttgcagaagt tcaagataaa attttaaaat ttgctccgac
     1381 tatgcctgtt gaaaaaaccg aattttacgt tgaattgttc cgtgaaaaat tagccgggct
     1441 attagtacct accagtagtg gtcatggttc tgtagacttg attctttctg agtgccataa
     1501 aactttcggc ttgaaaatgt tagttgagcg cttggggatt aatcctgacc agtgcgttgc
     1561 ttttggagat gggggcaatg acattgagat gttggagtat tgtggtctga gttatgaaat
     1621 ggataatgca acggaagctg ttaagcaagt tgctaagcat caatgtcctt caaatgacga
     1681 agacggtgtt ttagtgacgc tcgatcgatt gttccctaac acgtaaacac taggtaattc
     1741 atcaaaaaac ctcgatataa gaaatattcg aggttttaaa actatattta gaacagattt
     1801 acgaagcgtc tgatgagtca aaatagactt ggacattgct atctaaaata ccactttcat
     1861 tctttaaacg aattgtttga tttgatggag ttacctctaa tactaaatgc ccattaataa
     1921 aggtagtatc gtattcagat gttttaactg ttgcttcttc gcaaggtatt gttaaatcat
     1981 atgctggaat ataatgttgt gtggaaacta ataaaaaatg atcaccaatc atacagcatc
     2041 cctctgctga agaaaattga ttagagccat catttaaagt taacaagaat ccatccttta
     2101 aacgaggtac ttgcttaaat acttctggtt gtacttctaa aaataaatta tcgctcattt
     2161 tcacgaccac catcgacttc cacagttgaa attgacattt tatttaacac acctattccc
     2221 ccttaactat aactcaattc tatataaaaa atattctatt ctcaaataat tgaacttttc
     2281 aaatcgccaa tttacttcgg ctttatcaga gctcattcat atcaagagcg attaacgctg
     2341 taaaaattaa accgaagatc tgataaattt tctctatttt ctacaatatt taaaacgctt
     2401 ccttagtcta ttatatatct atttctctta ggagtatgtt tagatgtttg aataatagtt
     2461 gcccaacttt aaaggttatt cttataatat tttacttcat tttccaatag aaaattcaaa
     2521 ggattactgg tttttctaaa atagtttagt ttaaatacat aagtatcctc cgacactgta
     2581 ttagttcata atcatctaaa tacaaaattt tcaaataaaa atatttcaag ctctatcgtt
     2641 gacgaatgta aacgatactt ataaagtaat agaaattgat tacatttgta aacttaaaat
     2701 ggttaattta tctcacataa aactaagaat aaaataatga ttaaaagtgg ttagttttta
     2761 tacgatttag cctatcggta aaaatgaatt agagagtgta aaatattttg tgtaaatgaa
     2821 aaaatccata caaaaaagga agtc
//
LOCUS       DACEEZ010000162         2826 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20313_2017
            SAMN12639966-rid9879223.denovo.169, whole genome shotgun sequence.
ACCESSION   DACEEZ010000162 DACEEZ010000000
VERSION     DACEEZ010000162.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639966
            Sequence Read Archive: SRR10023158
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2826)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2826)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741683.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741683.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:44:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,016
            CDSs (total)                      :: 2,962
            Genes (coding)                    :: 2,889
            CDSs (with protein)               :: 2,889
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 36 of 73
            Pseudo Genes (incomplete)         :: 29 of 73
            Pseudo Genes (internal stop)      :: 18 of 73
            Pseudo Genes (multiple problems)  :: 8 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2826
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639966-rid9879223.denovo.169"
                     /isolate="E20313_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            7..624
                     /locus_tag="IWO89_002834"
     CDS             7..624
                     /locus_tag="IWO89_002834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0457522.1"
                     /translation="MGFQVLFGSLILTIVFLKWKTSNKKSMWSLVGIVLIIFTVTAAT
                     EANYSHKIQSYKGRLVMTKKDVTRYTSSRTGGLYDYAPLDALKYKDHLKKHEVKVGDR
                     WENASHKAYNSKIVYTVEDADEQNITLPVFNYWGTVVKVNGKKSTIENDNGLVSIKGT
                     GKRTIVEVSSTYPTTMILSLLISSITFIFLFTRYLIRCCGEMKID"
     gene            1644..2813
                     /locus_tag="IWO89_002835"
     CDS             1644..2813
                     /locus_tag="IWO89_002835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="HAQ0457523.1"
                     /translation="MPNFTTEIMETLINKGDLDELFRCHLELAVNSLLQAELTAFLDY
                     EKYDRAGFNSGNSRNGNYSRSFRTEYGELNLVIPRDRNGKFSQQTLPAYKRTNDSLET
                     TIIQLFQKGITMSEISELIEKMYGHHYTPQTISNMTKIVSEDIIAFKERSLESRYSVI
                     FMDATHIPLKRQTVSKEAVYIVIGIRLDGTKEVLGFSLAPTESAYVWKEILQDLKDRG
                     LKEVLLVVTDGLSGINDSIHSVYPNAQFQQCCVHISRNIAHKVRVSDRQEICSDFKLV
                     YQASSKEEANNQIRFMIDKWKKQYPRVVKLLMNPAILTFYNFPPSIRRTIYSTNLIEG
                     FNKQLKKYTKRKEQFPNEESLERFLVSQFNNYNQKFLCRVHKGFKEIHDTLESMF"
BASE COUNT         1051 a          378 c          447 g          950 t
ORIGIN      
        1 agaaccttgg gttttcaagt gctttttggt tcattaatct taaccatagt gtttttaaag
       61 tggaaaacaa gcaacaaaaa aagtatgtgg agccttgtag gaatagttct tattattttt
      121 actgtgactg cagcaacaga agcaaattat tcgcataaaa ttcagtcgta taaaggtcgt
      181 ttagttatga ccaaaaaaga tgttacacgt tatacatcct caagaactgg aggcctatat
      241 gactatgcac cattagatgc attgaaatat aaagatcatt taaagaaaca tgaagtaaaa
      301 gttggtgatc ggtgggagaa tgcatctcat aaagcatata attccaaaat agtctataca
      361 gtagaagatg ccgatgagca aaatatcaca ttacctgtat tcaattattg gggaacagtt
      421 gtgaaagtaa atgggaaaaa gagcactata gaaaatgaca atggtttagt gtctattaaa
      481 ggtacgggga aaagaactat agttgaggtt tcatctacgt atccaactac gatgattcta
      541 tctttattaa ttagttcaat aacttttata ttcttattta cacgttattt aataagatgc
      601 tgcggtgaaa tgaaaataga ttagatagat attataaata tgttgattct attttcatag
      661 caatggagaa aatacattag caatatataa aagaaggtat caagagtcac agtgaattta
      721 ttccgaaaag tgaaaagagc aaatataaaa tttaatgaaa atatagaact aaataggaaa
      781 acgtagacgt aattttctac gttttccttt tcatatatat taaatattac ggaaaattat
      841 ataaactaat ttagtgtttt gtttgtaatt cctaattttg ctccactttt agataatttt
      901 taggtctatt taactcatca gacattctat gctcattttt ctccaaattt gtttcaactc
      961 tttcgtactt aattttgcgt atttgaaaat tcttctttta gagtactttt attacgatac
     1021 tagtatgata tatatctata gcaaacagtt aaaatcttcc taaaataagc gaatagcatt
     1081 cctaaaacct ataaaatttt tagatgaagc gacaagtgga caattaataa actacttgaa
     1141 catgaaatta tggatcacct ttcaaattta gaaaatggaa ttcttatact tattgcgcaa
     1201 catttattga ttgcaaaatt atgtgacaaa aaaaactagt agaagaaaga ataatggctt
     1261 gataataaag tactacctat taaaggtagt actttattta atttagtata aattaacata
     1321 ctactatatt ttaggtttca tattatataa attaataata tactaccatt tgttggtagt
     1381 ataatttaaa ggtaataagt tattctttta ttactatgtt tgggttttat agcaaagaaa
     1441 gtttaataag agtttggacc tagcaaacaa tattaatgag gtcattaaat cttattatct
     1501 ttagtgatat aacgaaatat ctacacagac aaaatgatag cgtaaaatat tttgtgtaaa
     1561 tagatattta gggtagaaaa aaagaaagtc cattctgtaa aattaaagtt accacaaccg
     1621 aaattttata ggaggacttt ctcatgccca attttactac agaaattatg gaaacactaa
     1681 tcaataaagg tgatttagat gaattatttc gatgtcattt agaattagct gttaattcat
     1741 tattgcaggc tgaattaaca gcttttcttg attatgaaaa atatgatcgt gctgggttca
     1801 attcaggtaa ttcccgtaat gggaattatt cacgttcatt cagaacagag tatggagaat
     1861 taaatttggt aattcctaga gatagaaatg gtaagttttc tcaacaaact ttacctgctt
     1921 ataaaagaac caatgactct ttagaaacca ctattattca gctatttcaa aaaggaatta
     1981 ctatgtctga aatctctgaa ttgattgaaa aaatgtacgg gcatcactat acgccacaaa
     2041 ccatttcaaa tatgactaaa atagtttctg aagatattat tgcctttaaa gaaaggtcct
     2101 tagaatccag atactctgtc atttttatgg atgctactca cattccttta aaaagacaaa
     2161 ctgtttcaaa agaagcagtt tatattgtaa ttggcattcg attagatgga accaaagagg
     2221 ttcttgggtt tagtcttgct ccaactgaat ctgcttatgt ttggaaagag attcttcaag
     2281 acctaaaaga ccgtggttta aaagaggttt tattagtcgt aactgatggt ttaagtggca
     2341 ttaacgatag tattcatagt gtttatccaa atgctcaatt tcaacaatgt tgtgtccata
     2401 tatctagaaa tatcgctcat aaggttcgtg ttagtgatcg acaagaaatc tgtagcgatt
     2461 tcaaattagt ttatcaagca agttcaaaag aagaagcaaa taatcaaata aggtttatga
     2521 tagataaatg gaaaaagcag tatccacgag tggttaaatt gctcatgaat cctgctatat
     2581 taacctttta taatttccct ccgtcaatca gaagaactat ttattcaact aatttgattg
     2641 aaggttttaa caaacaatta aagaaatata ccaaaagaaa agagcaattt cctaatgaag
     2701 aatctctgga gagatttcta gtatctcaat tcaacaacta taatcaaaag tttctatgcc
     2761 gagttcataa aggttttaaa gaaatacatg atacattaga atcaatgttt taacttgatc
     2821 aacaga
//
LOCUS       DACEEZ010000163         2826 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20313_2017
            SAMN12639966-rid9879223.denovo.170, whole genome shotgun sequence.
ACCESSION   DACEEZ010000163 DACEEZ010000000
VERSION     DACEEZ010000163.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639966
            Sequence Read Archive: SRR10023158
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2826)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2826)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741683.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741683.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:44:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,016
            CDSs (total)                      :: 2,962
            Genes (coding)                    :: 2,889
            CDSs (with protein)               :: 2,889
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 36 of 73
            Pseudo Genes (incomplete)         :: 29 of 73
            Pseudo Genes (internal stop)      :: 18 of 73
            Pseudo Genes (multiple problems)  :: 8 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2826
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639966-rid9879223.denovo.170"
                     /isolate="E20313_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            798..1064
                     /locus_tag="IWO89_002836"
     CDS             798..1064
                     /locus_tag="IWO89_002836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0457524.1"
                     /translation="MAKITKNEQENLVENYTKKPAYSNSRLDAHFVCTSNPKLSFDAM
                     TIVGNLNKNSAKKLSDFMSLDPQIRLWDILQTKFKAKALQDPLC"
     gene            1122..1634
                     /locus_tag="IWO89_002837"
     CDS             1122..1634
                     /locus_tag="IWO89_002837"
                     /inference="COORDINATES: protein motif:HMM:NF013677.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003321317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HAQ0457525.1"
                     /translation="MTNKNNERYSTELKATAIKKMMPPENRGIRQLSQTLGIPEATLY
                     SWRKKARIQGNTTPGTKKNVEKWSSEDKFLIVMESFSMNQVELAEYCRKKGLFKEQIE
                     AWKKTCLSANEQEENRTRELATELKEEKKQARQLEKELRHKEKALAEAAALLLLRKKA
                     HAIWGDQEEE"
     gene            1631..2668
                     /locus_tag="IWO89_002838"
     CDS             1631..2668
                     /locus_tag="IWO89_002838"
                     /inference="COORDINATES: protein motif:HMM:NF033516.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HAQ0457526.1"
                     /translation="MISSSDRKQAVELIQEANRNGARLTYACNELNISVRTYERWTRE
                     GTIAHDQRPLAKRPVPKNKLTDQEREKIIETVSKKEFMNLPPSQIVPKLADCSIYIAS
                     ESSFYRVLREKNMQHHRGRSQVSQKRIPPSHLATKPNEVWTWDITWLKGPIKGLFYRL
                     YLIIDLFSRKMVGWEIWESEEARHAEKLIKQTVLKERIQGQPLVLHSDNGSPMKAATF
                     QVLLEKLGIQSSYSRPRVSNDNPYSEAAFRTLKYRPEYPNNGFKTIDDARKWTKEFVD
                     WYNDSHLHSGIQFVSPAACHEGRHLAILEKRKQVYEDAKKRHPERWSKGVRNWTPHQS
                     VALNPLKETES"
BASE COUNT         1022 a          496 c          564 g          744 t
ORIGIN      
        1 ttgatgacca cgaaagtgca tggcgatatt tgatgttgat actttatgca gtaccctatg
       61 tattcttcgc tatcattctg tggctcatag cattgttttt tagagtattt aatgaccttt
      121 aggtagattt atatataaaa agggctaaat acgcaaaata cccccttaaa atgcaaaata
      181 aatgcaaata agggggtaac tccttatgaa aaaacaacta atagtattat aacacgtttt
      241 tatgccgaga aaacttattg gttggaatgg gctatgtgtt agctaacttg ttagcgagtt
      301 ggttggactt gaattgggat taatcccaag aaagtaccaa ctcaacaaca cataaagccc
      361 tgtaggttcc gaccaataag gaaattggaa taaagcaacg aaaggagttg agtaaaatga
      421 gtgagaaaac gaagaaaatc attcgagtat cattagcttt ttcacaagta gtagtgcaat
      481 cattgatagt ttattatact taccagcaat ataaattatc taaagagaat taagttttga
      541 ttatgtaata agttttggta ctaaaaaaga cttgatctaa ttagaccaag tcttttgtta
      601 gtgttatatt aataacaaaa taaaaataag aagtcgctca ctccccgacc aaagtttgtg
      661 agcaaaataa acagtcgatg tttgtttctt aattgtatat cttgatatta aacgatttaa
      721 gattattctt caagatatat attcgcaaat gtaattggaa aagcgacttc ttaaacaaaa
      781 ttaaggagtc gctttttatg gcaaaaataa cgaaaaatga gcaagaaaat ttggtggaaa
      841 attacacaaa aaaaccggcg tactctaata gccggttaga cgcacatttc gtgtgcactt
      901 ctaatccgaa attgagcttt gacgctatga cgatagtcgg aaaccttaat aaaaacagcg
      961 ctaaaaagct atccgatttt atgagtttag atccacaaat tagactttgg gatatacttc
     1021 aaacaaaatt taaagctaaa gcactacaag accccctatg ttaggctagt tgtcgtatag
     1081 gataataagt tataataaag actatacgga ggatgattac catgacaaat aaaaacaatg
     1141 aacgttactc taccgaactg aaagcgacag ccatcaagaa aatgatgcca cccgaaaata
     1201 gggggattcg ccaattaagt caaactttgg gcattccaga agcgactctt tattcttggc
     1261 gtaaaaaagc tagaattcaa gggaatacta cacccggaac gaaaaaaaat gtcgaaaaat
     1321 ggtcaagtga agataaattt ttaattgtga tggaatcatt ctcaatgaac caagtagaac
     1381 ttgcggaata ttgccgaaag aagggcttat ttaaagaaca aattgaggct tggaagaaaa
     1441 cgtgcttatc tgcgaatgaa caggaagaaa atcgaacgag agaacttgca actgaactaa
     1501 aagaagagaa aaaacaagcg cgtcaattag agaaagaatt acgtcataag gaaaaagcat
     1561 tagcggaagc cgctgctctc ttattactaa gaaaaaaagc acacgcgatt tggggggacc
     1621 aagaggaaga atgatttcat cttcagatcg caaacaagct gttgaactta ttcaagaagc
     1681 caatcgcaat ggggcccgtc tcacctatgc ctgtaatgag ctgaatatta gcgttcgaac
     1741 ctacgaacga tggacgcgtg aaggtacgat agcgcatgat caacgccctt tggcgaaacg
     1801 tcctgtaccc aaaaataaac tgacggatca agaacgcgaa aaaataatag aaaccgtttc
     1861 aaaaaaagaa tttatgaatt tgccacctag ccaaattgta ccaaaacttg cggattgctc
     1921 gatttatatt gcatcagagt ctagcttcta tcgtgtattg cgagaaaaaa atatgcaaca
     1981 tcaccgtggc cgaagtcaag tttcacaaaa acgcataccg ccaagtcacc ttgcgacgaa
     2041 gccaaatgaa gtttggactt gggatattac ttggctaaaa ggtccaataa aaggactctt
     2101 ttaccggtta tatctcatca ttgatttatt tagccgtaaa atggtaggct gggagatttg
     2161 ggaatcagaa gaagctcgtc atgccgaaaa acttattaaa cagacggtgc ttaaggaacg
     2221 tatccaagga cagccgttgg ttttacactc cgataatggc agccctatga aagccgcgac
     2281 ttttcaagtc ttactcgaaa aattaggaat tcaaagttct tattcaagac cacgagtgag
     2341 taatgacaat ccttattcgg aagcggcttt ccgaacgtta aaatatcgac cagaatatcc
     2401 caataacggc tttaaaacaa tagatgatgc tcgaaaatgg acgaaagagt ttgtcgattg
     2461 gtataacgat tctcatttgc atagtgggat tcaatttgtg tctcctgcag cgtgtcatga
     2521 aggacgacat ctcgctattt tagagaaacg aaagcaggtt tacgaggacg ctaaaaagcg
     2581 ccatcctgaa cgctggtcaa aaggtgttcg aaattggact ccacatcaat cggtggcgtt
     2641 gaatccttta aaagaaacag aaagttaaca ggagcataat ctttcaagat tatcaaataa
     2701 ccctcgtcac ccaatagggg aaaaagttat tcttccccta ttggataacg aggccaaccg
     2761 accataggga ggttctgctt tttgataggt ggaaaataaa aatacgacaa ctatattgac
     2821 aaacac
//
LOCUS       DACEEZ010000164         2821 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20313_2017
            SAMN12639966-rid9879223.denovo.171, whole genome shotgun sequence.
ACCESSION   DACEEZ010000164 DACEEZ010000000
VERSION     DACEEZ010000164.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639966
            Sequence Read Archive: SRR10023158
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2821)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2821)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741683.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741683.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:44:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,016
            CDSs (total)                      :: 2,962
            Genes (coding)                    :: 2,889
            CDSs (with protein)               :: 2,889
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 36 of 73
            Pseudo Genes (incomplete)         :: 29 of 73
            Pseudo Genes (internal stop)      :: 18 of 73
            Pseudo Genes (multiple problems)  :: 8 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2821
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639966-rid9879223.denovo.171"
                     /isolate="E20313_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            95..1054
                     /locus_tag="IWO89_002839"
     CDS             95..1054
                     /locus_tag="IWO89_002839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30-like element IS1252 family transposase"
                     /protein_id="HAQ0457527.1"
                     /translation="MTYTHLTTDELVMIESYYHQNIKIVKIAEYLKRSRTPIYNVINF
                     LKEGHTALEYYQQYKENKKRCGRRNIVLPKKQQSYIKEKIAQGWTPDVIIGRKEKPIS
                     CSVRTLYRQFKEKVFDESTLPMKGKRKPNGHQERRGKQAFKRNIVERETDYPTFKEEF
                     GHIEGDTIVGIHHKSAVITLVERLSKIIITLKPEGRKAIDIENTLNHWFGSIPRHLFK
                     SITFDCGKEFSNWKALSNQHDIAIYFADPGTPSQRALNENSNGLLRKDGLPKNMDFNQ
                     VDQAFISSVANKRNNIPRKSLDYQTPLEVFMSYMNEDILSSLI"
     gene            complement(1487..2059)
                     /locus_tag="IWO89_002840"
     CDS             complement(1487..2059)
                     /locus_tag="IWO89_002840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000170424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HAQ0457528.1"
                     /translation="MTKYGYARVSTASQSTKEQIQQLLENGVEKKNIFSEKFTGTKRD
                     RPKFNQLLAGLKPGDEIVVTKLDRFARNTREALEIIEPLLDDNVTIKVLNLGTIENTS
                     MGRMVTRTLLSVAEMERDMIVERTQEGKMFARKNNPNFREGRPKATITPKKRHAYELL
                     TSGKSYKEVEAITGFSRSTLFRIKKKIEES"
     gene            complement(2075..2680)
                     /locus_tag="IWO89_002841"
     CDS             complement(2075..2680)
                     /locus_tag="IWO89_002841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell filamentation protein"
                     /protein_id="HAQ0457529.1"
                     /translation="MILENKLGLTNQVELAKVEEKLSKQKAKELYDCGKINEIEVGTF
                     KGLSEIHEFLFSDIYDFAGKIRSVNIAKGNFRFAPVMYLEHSLQHIDQMPQTSFEEII
                     KKYVEMNIAHPFREGNGRSTRIWLDLILKEELQKVVDWNLIDKADYLSAMERSPINDL
                     EIRYLISNALTDKIDDRELYMKGIDVSYFYEGYSEYTIDDL"
BASE COUNT          888 a          579 c          469 g          885 t
ORIGIN      
        1 gccgattgta aaattaagct agacaaataa ataagccatt cgtgctacac tcaaaatgta
       61 attccgacta aagaaaacat aggagtgatc acgaatgacc tacacccatc ttaccacgga
      121 tgaactggtg atgatagagt cttactacca ccaaaacatc aaaattgtga aaattgctga
      181 gtacctgaaa cgatctcgaa caccgattta taacgtcatt aatttcttga aagaaggcca
      241 tacggccctc gaatactacc aacagtataa agaaaataag aaacggtgtg gcagacgaaa
      301 catcgtcttg ccaaagaaac aacagtccta cattaaagaa aaaattgccc aaggctggac
      361 gccagacgtt atcattgggc gtaaagagaa accaatcagc tgttccgtaa gaacccttta
      421 tcgccagttc aaggaaaagg tgttcgatga atcgactttg cccatgaagg gtaagcggaa
      481 accaaatgga catcaagaac gccgaggaaa acaggctttt aaacggaata tcgttgaaag
      541 agaaactgat tacccgacat ttaaagaaga gttcggccat atcgaaggcg ataccattgt
      601 aggcattcat cataagagtg ctgtcataac tttggtagaa cgtctatcga aaatcatcat
      661 caccttgaag cccgaggggc gcaaggcgat cgatattgag aatactctga accactggtt
      721 tggatcgatt cccagacatt tatttaaatc cattaccttt gattgtggga aagaattctc
      781 taattggaag gctttaagta accaacatga catcgctatt tatttcgctg atccagggac
      841 tccttcgcaa cgagcactga atgaaaattc caatggcttg ctgcgaaaag atggtctgcc
      901 aaaaaacatg gattttaatc aagtcgacca agcttttatc tcttcagtgg cgaataagag
      961 aaacaatatt ccacgaaagt ccttggatta tcaaacaccc ctggaagtat ttatgagtta
     1021 catgaatgaa gatattttgt ctagcttaat ttgacaaatc aaattattta aaactttgca
     1081 acagaacctt agcagcagtg ctgattttta tgatagttat ttggttctta aatgaaagat
     1141 ctaaagacaa tcaacaccgt agggatatgg agataaaatc aatagagtca atagattatt
     1201 tatcgataat aacactaaaa aagccacgtt cctaaacgtg acttacgctg aacgatatgt
     1261 gttgtttggt agacgaacaa atcgttcatt ataaatatat gatggtatcc taatatagta
     1321 agcgtggcta gcataccttt aattacttta ccttttacag ttaccataca cattttttga
     1381 gcagaaatca agtaaataaa aaaatattat atttcacaac atgctaatag actcatatct
     1441 agaacgcatt aagtatttac ctgtctggtt cgctgagact tcttttttaa ctttcttcaa
     1501 ttttcttctt tattctaaaa agcgtactgc gactgaaacc ggttatagct tcaacttcct
     1561 tatacgattt acctgaagta agcagttcat atgcgtgcct cttcttcggt gtgattgttg
     1621 ctttcggacg tccttctctg aaattaggat tattctttct tgcaaacatt tttccttctt
     1681 gggtacgttc cacaatcata tcacgttcca tttctgctac actcaacaac gtccgtgtca
     1741 ccattcgccc catggacgta ttttcaatcg ttcctagatt caaaaccttg atcgttacgt
     1801 tatcatcgag taaaggttca atgatctcta atgcttctct cgtgttacga gcaaatcgat
     1861 caagcttcgt aacaactatt tcatctccag gcttcagacc agccaacagc tgattgaatt
     1921 ttggccgatc ccgttttgta cccgtaaatt tttctgaaaa aatattcttc ttctctacgc
     1981 cgttctcaag caactgttga atttgttctt ttgtactttg agaagcggtt gagacacgtg
     2041 catatccata tttcgtcatt tggtccacct acttttacaa atcatctatc gtatactcag
     2101 aatacccttc atagaaatag ctgacatcga ttcctttcat atataactcg cgatcgtcaa
     2161 ttttatcagt cagagcattg gaaattagat agcgtatttc caaatcgttt atgggacttc
     2221 gctccattgc agaaagatag tctgctttat cgattaagtt ccaatctaca actttttgca
     2281 attcttcctt taaaatgaga tccagccaaa ttctagtact tcggccattt ccttctcgaa
     2341 atgggtgagc gatattcatc tccacatatt tcttgataat ctcttcaaaa gaagtttgtg
     2401 gcatctgatc aatatgctgt aacgaatgtt ctaaatacat aactggtgca aatcgaaaat
     2461 ttccttttgc aatattgacc gatcggattt ttccggcaaa atcgtaaata tccgaaaata
     2521 gaaattcatg aatttcagat aaacctttaa atgtaccaac ttcgatttca tttatttttc
     2581 cacaatcgta caattctttc gctttttgtt tactgagttt ttcttcaacc ttagccaact
     2641 caacttgatt ggttaatccc aatttattct ctaaaatcat gcgaaatctc cttaacttta
     2701 tgacataagt ttatgaaaca ctctcaaccc cattatatcg cgattttgtt tatgtgtcaa
     2761 aaactattaa gtttctgacc cccgatagat ttttcaagtg tctctatttt gcacagtcta
     2821 g
//
LOCUS       DACEEZ010000165         2739 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20313_2017
            SAMN12639966-rid9879223.denovo.172, whole genome shotgun sequence.
ACCESSION   DACEEZ010000165 DACEEZ010000000
VERSION     DACEEZ010000165.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639966
            Sequence Read Archive: SRR10023158
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2739)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2739)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741683.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741683.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:44:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,016
            CDSs (total)                      :: 2,962
            Genes (coding)                    :: 2,889
            CDSs (with protein)               :: 2,889
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 36 of 73
            Pseudo Genes (incomplete)         :: 29 of 73
            Pseudo Genes (internal stop)      :: 18 of 73
            Pseudo Genes (multiple problems)  :: 8 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2739
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639966-rid9879223.denovo.172"
                     /isolate="E20313_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            700..2610
                     /gene="ltrA"
                     /locus_tag="IWO89_002842"
     CDS             700..2610
                     /gene="ltrA"
                     /locus_tag="IWO89_002842"
                     /EC_number="2.7.7.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="HAQ0457530.1"
                     /translation="MPKNKNETLCVEDLRHAEYYEMQNTFDDLYAKSKNGDIFTHLMD
                     IILSRENILLAYRNIKANAGSKTAGTDGTIIKDIGKLPAETVVKKVRYIVAGSPHGYR
                     PKPVRRKEIPKPNGKTRPLGIPCMWDRLIQQCIKQVLEPICEAKFSENSYGFRPNRSV
                     ENAIKATYNRLQISQLHYVIEFDIKGFFDNVNHSKLIKQIWAMGIRDKHLIFILKRIL
                     KAPIKMTNGTITYPEKGTPQGGIISPLLANIVLNELDHWVESQWQENPVTKNYVVHIN
                     KSGSPCKSNAYKEMKKTKLKEMYMVRYADDFRVFCRYKESAEKAKIAITQWIEQRLKL
                     EVSQEKTRIVNVRKRYSDFLGFKIKMIPRRKKLVVKSHISDKQFKNQKDKLVSQAKKI
                     ASPSKGKTELEETRLYNSMVLGMQNYYQIATNVNLDCMKLNRAVMAIFTNRLGTNKRG
                     RLRKTGRQLTTKEATRYGKSKMLRYVAGEGEPIFPVGYIQHRKPMAKVYLANCYTLEG
                     RKFIHTNLSVDKLLMYQLMKLSLENRTIEYADNRISLFSAQHGKCAITLEEFQQANEI
                     HCHHIVPTGAGGNDDYKNLILVKEAVHRLIHAKTEETIQKYIALLKLNKVQLIRLNKY
                     RVLAGNQELNLI"
BASE COUNT         1072 a          425 c          558 g          684 t
ORIGIN      
        1 aaaagctaag gcactaataa aagaacgaaa aagtttggtt attaggagaa ctgataatct
       61 gacaggtgga atgatgaagt aacgctctga aacgcctgcc ctgagcctcc gacaagcatt
      121 gtatgaggaa actcagaatg tttgaagctc ggtaaagacg gctgaaagat accgtaatag
      181 ttgatgaaca tatatcttct atcgaaaact atccagaggt gggtagcgta tccaacaggt
      241 cgggggtcta taaaatatct atggttagaa tgttctataa ggaactgacg aaacagcgaa
      301 tgtacgggtc taaatttgaa ctttgtagaa atgcaaaggt acgagaaatc gtagctggtg
      361 tggcaaagta agaatatagg ttatgaaatg ccatataacg ttacaggcgg tatcaagcca
      421 gcaggcttat agctagtgac ctaaggatat atatgtatag ataagattat cggaacgagg
      481 aaaggtattg ggttctcatc aagagaataa actgacgaag aacaataagc agttgaacta
      541 atgctgaaaa gcagagggcg aacctaagaa attcttgtaa tgagaatgga ggaatgccct
      601 caagtcgtgt atatattaga aaagtattat gttgtttcaa taaggattac gagtaagata
      661 aaaaagctca ctcaatttca agtaaaggag tgaagcctta tgcctaaaaa taaaaatgaa
      721 acattgtgtg tagaagattt aagacacgct gaatattacg aaatgcaaaa cacctttgat
      781 gatttatatg ccaaaagtaa gaatggagat attttcactc atcttatgga tattatccta
      841 tcgagagaaa atatattact ggcatatcga aatatcaaag caaatgcagg cagtaaaaca
      901 gcaggaacag acggtacaat aattaaagat attggcaaat tgccagcaga aacagttgtc
      961 aaaaaagtaa gatacattgt tgcaggcagt ccacatgggt atcgacctaa gccagtgagg
     1021 cgaaaagaaa taccaaaacc aaatggtaaa accagacctc taggtattcc ttgtatgtgg
     1081 gacaggctta tacaacaatg tataaaacaa gtattagaac caatctgcga agctaaattt
     1141 agtgaaaaca gctatggttt tagacctaat aggtcagttg aaaatgcaat aaaagcaacc
     1201 tataaccgcc tacaaatcag ccagctacat tatgtgattg aatttgatat taagggtttc
     1261 tttgacaacg tgaaccattc caagctgata aaacagatat gggctatggg aatacgggac
     1321 aagcacttga tattcatttt aaaaaggata ttaaaagctc caatcaaaat gactaacggt
     1381 acaataacat atccagagaa aggaactccg caagggggaa taatatcccc actactagca
     1441 aacattgttc ttaatgagtt agaccattgg gtagaaagcc aatggcagga aaatcctgtt
     1501 acaaaaaact atgtggttca tatcaataaa agcggtagcc catgtaaaag caacgcttac
     1561 aaagaaatga agaaaacaaa gttaaaggaa atgtatatgg ttcgttatgc agatgatttt
     1621 agagttttct gccgctacaa ggaaagtgct gaaaaagcca aaatagcaat cactcaatgg
     1681 attgaacaac gattaaaatt ggaagtatca caagaaaaaa caagaattgt aaatgttaga
     1741 aaaagatact ccgactttct tggtttcaag attaaaatga taccgagaag aaagaagtta
     1801 gttgttaaat cacatataag tgataaacaa ttcaaaaacc aaaaagacaa gctggtatca
     1861 caggcaaaga aaattgcttc tccctcaaaa gggaaaacgg aattagaaga aacaagactt
     1921 tataattcaa tggtattagg tatgcaaaat tattatcaga ttgcaacgaa tgtaaatctg
     1981 gattgtatga aactgaatag agctgtaatg gctatcttca ctaacagatt aggtacaaac
     2041 aaaaggggca gattgagaaa aacaggcaga caacttacga caaaggaggc aacaagatat
     2101 ggtaaatcta aaatgcttcg gtatgttgct ggcgagggcg aacctatttt ccctgttgga
     2161 tatatccaac acagaaaacc aatggcaaag gtgtatttag cgaattgcta cactctagag
     2221 ggaagaaaat ttatccacac caatctaagt gttgataaat tactgatgta ccagttaatg
     2281 aaattatctt tggaaaatcg cacaatagaa tacgcagaca atcgaatttc gctattttct
     2341 gctcaacatg gaaaatgtgc tataactctg gaagaatttc aacaagctaa tgaaatacat
     2401 tgtcatcata ttgtaccaac tggagctggt ggaaatgacg attataaaaa tcttattttg
     2461 gtaaaagagg cggtacaccg tctaatacac gctaaaacag aagaaacaat acaaaaatat
     2521 atagctctat tgaagctaaa taaagtccag ctcataagac taaataaata tagagtatta
     2581 gctggcaatc aagaattaaa cttgatatag gtctactaca taaatacttt tcaatataaa
     2641 cacgatggaa cgcggagtgc ggtgaaagtc gcacgctccg tgtgaatggg gggaaaagtt
     2701 tgagatattt ataatatcag gaacttacct atccatata
//
LOCUS       DACEEZ010000166         2455 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20313_2017
            SAMN12639966-rid9879223.denovo.173, whole genome shotgun sequence.
ACCESSION   DACEEZ010000166 DACEEZ010000000
VERSION     DACEEZ010000166.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639966
            Sequence Read Archive: SRR10023158
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2455)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2455)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741683.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741683.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:44:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,016
            CDSs (total)                      :: 2,962
            Genes (coding)                    :: 2,889
            CDSs (with protein)               :: 2,889
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 36 of 73
            Pseudo Genes (incomplete)         :: 29 of 73
            Pseudo Genes (internal stop)      :: 18 of 73
            Pseudo Genes (multiple problems)  :: 8 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2455
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639966-rid9879223.denovo.173"
                     /isolate="E20313_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            132..344
                     /locus_tag="IWO89_002843"
     CDS             132..344
                     /locus_tag="IWO89_002843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014834536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0457531.1"
                     /translation="MAVWQIQLKERGAYGLISLSDVLCRKEMPDLFSLDQIIAIIGAA
                     AVVITAVAKASDSILAWLKFKKEQKK"
     gene            506..1024
                     /locus_tag="IWO89_002844"
     CDS             506..1024
                     /locus_tag="IWO89_002844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000357244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfbU family protein"
                     /protein_id="HAQ0457532.1"
                     /translation="MDKFNRLTLINQFEILKALNPNEEKYYAEKIEILTEGYEYHYSE
                     IFENISDPLPEEDSRFVLDILNMYRDITFSKNKLKDINSIDNYYIQFKGFDFNSSTEF
                     KLALYAQFFIEKLNRFQEIVEDSDFNTFNSHKPMRGTYIKFLENYNDLKSTNNYQFGN
                     LSYEMISKVLSL"
     gene            1031..1543
                     /locus_tag="IWO89_002845"
     CDS             1031..1543
                     /locus_tag="IWO89_002845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000774078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0457533.1"
                     /translation="MKLNSINDLFAIIGVIANILFSCLNFRFTQKNKKNKLSIEQMKK
                     DIETDLKNDHKERRKIEEYTSLMEKCKATDAIFSFLRKSDEIITRENIDLENEISKLE
                     SIFSDINSLKYLIVEENSDSFLEFEKYLTDLIDNRNNELMRQNIARFLTKLVSEWKSI
                     ISQNMDKLRY"
     gene            1814..2437
                     /locus_tag="IWO89_002846"
     CDS             1814..2437
                     /locus_tag="IWO89_002846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HAQ0457534.1"
                     /translation="MYLAYLRVSSDDQSLARQHKLIENWSVKNNIAEDDLKIFEEKVS
                     GKNIENRPELQELISFIRERDTIIIPSLDRLARNSKDTKDLLQLFRSKGASIEILDLP
                     SFNGVTDPALRDLLTNLVIEVFSYVAENERKKIKERQKQGIEIAKKNGRYTGKKIQYH
                     ANATGKNKLIYDEIVDGLKKKETISKIAKNAGVTRKTVYRVKRDLGM"
BASE COUNT          944 a          310 c          409 g          792 t
ORIGIN      
        1 aaaacactat atattccgaa aagtgagtat ctccattcat aaaataatca acatatttaa
       61 aaaatctaat tagctttttt agtcaatcta aagttcttta ttctatgata taatgttact
      121 gtagaaagtt tgtggcggtt tggcaaatcc aattgaagga gcgtggtgcc tatgggctta
      181 ttagcttatc ggacgtactc tgtagaaagg aaatgcctga tttgttctca ttggatcaaa
      241 tcattgctat tatcggagca gcagcagtag ttattactgc cgtagcgaaa gctagcgatt
      301 cgattttagc ttggttaaag ttcaaaaaag aacaaaaaaa atagagccgt ctcaactttt
      361 ggcgaggtag acgagctcta aagctttatt tacatgccat ccgctttgaa cggactacac
      421 tgggcatgtg acagcatgcc ctttcattta tattatatct cattctttta tataatgaaa
      481 gtaaataaat catggaggat aaaatatgga taaatttaat agattaacat taataaatca
      541 gtttgaaatt ttaaaagctt taaatcctaa cgaagaaaaa tactatgctg aaaaaataga
      601 aatactaaca gaaggttacg aatatcacta tagtgaaata tttgaaaata tttctgatcc
      661 attgccagaa gaagattcgc ggtttgttct agacatatta aatatgtata gagatattac
      721 ttttagtaaa aataaattaa aagatataaa ttcaattgat aattattata tacaatttaa
      781 aggttttgac tttaatagct ctaccgaatt taagttagca ctttatgcac aattttttat
      841 tgaaaaatta aataggtttc aagaaatagt tgaagatagt gattttaaca cttttaatag
      901 ccataaaccg atgagaggta cttatataaa atttctcgaa aattataatg atttaaaatc
      961 taccaataat tatcaatttg gaaacttgtc ttatgagatg ataagtaaag ttctaagttt
     1021 gtgatgataa atgaaattaa atagtataaa tgatttattt gctattatag gtgttattgc
     1081 taacatactt ttttcgtgtt tgaattttag atttactcaa aagaacaaaa agaacaaact
     1141 cagtatagaa caaatgaaaa aagacattga aactgattta aaaaatgacc ataaagaaag
     1201 aagaaaaatt gaagaatata cttctttaat ggaaaagtgt aaagcaactg atgcaatttt
     1261 tagcttttta agaaaaagtg atgagataat cacacgtgaa aatatagatt tagaaaatga
     1321 gattagtaaa cttgaatcta tcttttccga tataaattct cttaagtatt taatcgtcga
     1381 agaaaattcg gatagctttt tagaatttga aaaatatcta acagatctta tagataatag
     1441 aaataatgaa ttaatgagac aaaacattgc aagattcctt actaaattag tttctgaatg
     1501 gaaaagtatt atttctcaaa atatggataa attaagatat taaggatgtt agttttaaaa
     1561 taaactaatg tccttatttt tgtgttcaca tttctgttac ctaagaaata cttaaaggag
     1621 gtgatgtagc cagcaacgac tacaacttga acccgctatc tacagtttat ttgttgtttc
     1681 ttttgggtat actctaatgg tacaaatcca tatttgctta aagcgttgat ttttcagcgg
     1741 tttttcttta ttgcaaagtg taccctaaga cctgtatact aatagtaaat agattggata
     1801 ggaatgatgg caaatgtatt tagcttattt aagagtttct tccgacgatc aaagtttagc
     1861 tcggcaacat aagctaattg aaaattggtc agtcaaaaat aatattgctg aagacgattt
     1921 aaaaattttc gaggaaaaag tctctggaaa aaatattgaa aacagaccag agctgcaaga
     1981 gcttatttct ttcatacgtg aacgagatac gattattatt cctagtcttg atcgattagc
     2041 aagaaatagt aaagatacaa aagacttact tcaattgttt cgtagtaaag gtgcttctat
     2101 tgaaatatta gacctaccgt cgtttaatgg ggtaactgac cctgcattaa gagacttgct
     2161 aacaaattta gtcatagaag tgtttagcta tgttgctgaa aacgaacgga aaaaaatcaa
     2221 agagcgtcaa aaacaaggca ttgaaattgc aaagaaaaat ggtcgttata ctggtaaaaa
     2281 gattcaatac catgccaatg caacaggaaa aaacaaattg atttacgatg aaattgttga
     2341 tggtctgaag aaaaaagaaa ctatttctaa aatagcaaag aacgcaggag ttacaagaaa
     2401 aaccgtttat cgagtaaaaa gagatttagg aatgtaatat gttccagtta gaaag
//
LOCUS       DACEEZ010000167         2407 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20313_2017
            SAMN12639966-rid9879223.denovo.174, whole genome shotgun sequence.
ACCESSION   DACEEZ010000167 DACEEZ010000000
VERSION     DACEEZ010000167.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639966
            Sequence Read Archive: SRR10023158
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2407)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2407)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741683.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741683.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:44:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,016
            CDSs (total)                      :: 2,962
            Genes (coding)                    :: 2,889
            CDSs (with protein)               :: 2,889
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 36 of 73
            Pseudo Genes (incomplete)         :: 29 of 73
            Pseudo Genes (internal stop)      :: 18 of 73
            Pseudo Genes (multiple problems)  :: 8 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2407
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639966-rid9879223.denovo.174"
                     /isolate="E20313_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            5..1150
                     /locus_tag="IWO89_002847"
     CDS             5..1150
                     /locus_tag="IWO89_002847"
                     /EC_number="2.5.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine gamma-synthase"
                     /protein_id="HAQ0457535.1"
                     /translation="MHIQTKLIHGGISEDPTTGAVSVPIYQTSTYRQDGVGQPKQYEY
                     SRSGNPTRFALEELIADLEGGVRGFAFSSGLSGIHAVFSLFQAGDHILLGDDVYGGTF
                     RLFDKVLTKNGLEYTIIDTSNLDKIEQSIKPNTKALYLETPSNPLLKITDLEKSATLA
                     HQHGLIVIADNTFATPYFQRPLDLGSDIVVHSGTKYLGGHSDVVAGLVTSNHKDLADQ
                     IGFYQNAIGAVLGPQDSWLLQRGIKTLSVRMEEHQKNAFVVADFLFSHPAVEKVYYPG
                     LPDHELHGVAKQQMSGFSGMISFTLKNEESAIPFVESLQLFTLAESLGGVESLVEIPS
                     VMTHASIPKEKREEAGIKDGLIRLSVGIEYGQDLINDLAQAFDRIKN"
     gene            1321..1779
                     /locus_tag="IWO89_002848"
     CDS             1321..1779
                     /locus_tag="IWO89_002848"
                     /EC_number="4.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-ribosylhomocysteine lyase"
                     /protein_id="HAQ0457536.1"
                     /translation="MRMNVESFNLDHTKVKAPYVRVADRKTGAHGDVIIKYDVRFKQP
                     NKEHMDMPSLHSLEHLTAELIRNHADYIVDWSPMGCQTGFYLTVLNHESYEDILDVLE
                     ATMKDVLEATEVPASNETQCGWAASHTLEGAQALAREFLAKRDEWSEVEA"
     gene            1938..>2407
                     /locus_tag="IWO89_002849"
     CDS             1938..>2407
                     /locus_tag="IWO89_002849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione peroxidase"
                     /protein_id="HAQ0457537.1"
                     /translation="MSIYNFETTLEDGTVYSLKKYAGKPIIIVNTATKCGLAPQFKQL
                     EELYQAYQTQGLVILGFPSNQFKQELDSATEAAEACKTTYGVTFPMHQLIDVNGENTD
                     PLFTYLKNEAPGTVTKAIKWNFTKFLIDRNGKVIERFAPQTSPLKMTAAIESVL"
BASE COUNT          802 a          452 c          455 g          698 t
ORIGIN      
        1 aaacatgcac attcaaacaa aattgatcca cggaggcatc agtgaagatc ccacaacagg
       61 agctgttagt gtacctattt atcaaacatc cacttatcgg caagatggcg tcggccagcc
      121 taaacaatat gaatattcaa gatctggtaa tcctactcgt tttgcattgg aagaactgat
      181 tgctgatttg gaaggcggtg ttcgaggatt cgctttttct tctggtcttt ctgggattca
      241 tgccgttttt tctctttttc aagctggcga tcatatctta ttaggagatg atgtctatgg
      301 aggaacattc cgtctatttg acaaagttct tactaaaaat ggtctcgagt acacgatcat
      361 agatacgagt aatcttgata aaatagaaca atccatcaaa cccaatacaa aagcacttta
      421 tcttgaaaca cctagcaatc cgcttctaaa gattactgat ttagaaaaaa gtgccactct
      481 ggctcatcaa catggattga tagttattgc tgacaataca tttgctactc cttattttca
      541 aagaccttta gatctcggtt ctgatatcgt tgtccatagc ggtaccaaat atctaggcgg
      601 acacagcgat gtagtagctg gtttagtgac ttctaaccat aaagatctag ccgatcagat
      661 cggattttac caaaatgcta tcggagccgt acttggtccc caagatagct ggttacttca
      721 gcgcggaatc aagacactaa gtgttcgtat ggaggaacat caaaaaaatg cttttgtggt
      781 agctgatttt ctattttctc atccagcagt agaaaaggtc tactatccag gattacccga
      841 tcatgaactc catggagttg ctaagcagca gatgagcggc tttagcggaa tgatttcttt
      901 tactttaaaa aatgaagaat ctgctattcc atttgttgaa tcactccaac tatttacttt
      961 agcagaaagt ttaggtggcg tagaaagttt agtggaaatt ccttctgtca tgactcatgc
     1021 gtctattcca aaagaaaaac gagaagaggc tggcataaag gacggcttga tccgtctttc
     1081 tgtaggaatt gagtatggtc aggatctcat aaacgattta gctcaggcat ttgacagaat
     1141 caagaactag aagtcccaat aaacgactta gcagaaaaat ctaaaaaata tttcagattg
     1201 ctacaccttg gaaacgataa gtgttagaat caagtagaaa gacgtttttg taaatttcac
     1261 taaataattc tttagtgaaa agcatcaaaa cgaatgaaca aacagatagg agtcttacaa
     1321 atgagaatga atgtcgaaag ttttaatctt gaccatacaa aagtcaaagc accttatgta
     1381 cgtgtagctg atcgaaaaac gggtgcgcat ggcgatgtca tcattaaata cgacgtccgc
     1441 tttaaacaac caaataaaga acacatggac atgccaagtc tacattcact tgagcacttg
     1501 actgccgaac taatccgcaa tcacgcagat tacatcgtag actggtctcc aatgggatgc
     1561 caaacaggct tttacttgac tgtattgaat catgaaagct acgaagacat tttagatgtt
     1621 cttgaagcaa caatgaaaga tgttcttgaa gctactgaag tacctgcaag caatgaaaca
     1681 caatgtggct gggctgccag ccatacacta gaaggtgcac aagctttggc tcgtgaattt
     1741 ttagcaaaac gcgatgaatg gtctgaagta gaagcatagt ttctaaaaga acgtaaagat
     1801 gagaagcaat atagctatct tatcttcgta ttttattaaa tatttattgt ctgaaaagag
     1861 ttctaaacgg gaaaacaaat agctagtttt cagtttagcg ctctttttat tatataaaat
     1921 agaaaggaga aaactttatg tctatttata attttgaaac tactttagaa gacggcactg
     1981 tctattcatt gaaaaaatat gcaggcaaac cgataatcat tgttaacact gcaacaaaat
     2041 gcggtctagc acctcaattt aaacaattag aagaacttta ccaagcttat cagacacaag
     2101 gtctggttat cttaggcttt ccttccaatc aatttaaaca agagctagat tcagcgactg
     2161 aagcagcaga agcttgtaaa accacttatg gcgttacctt ccctatgcat caattgattg
     2221 acgtaaatgg agaaaataca gatcctcttt ttacttatct taaaaatgaa gctcctggta
     2281 ctgtcactaa agcaataaaa tggaatttta ctaaattttt gattgataga aatggcaaag
     2341 taatcgaacg atttgcacca caaacttctc cattgaaaat gaccgctgca attgaatccg
     2401 ttcttta
//
LOCUS       DACEEZ010000168         2398 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20313_2017
            SAMN12639966-rid9879223.denovo.175, whole genome shotgun sequence.
ACCESSION   DACEEZ010000168 DACEEZ010000000
VERSION     DACEEZ010000168.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639966
            Sequence Read Archive: SRR10023158
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2398)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2398)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741683.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741683.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:44:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,016
            CDSs (total)                      :: 2,962
            Genes (coding)                    :: 2,889
            CDSs (with protein)               :: 2,889
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 36 of 73
            Pseudo Genes (incomplete)         :: 29 of 73
            Pseudo Genes (internal stop)      :: 18 of 73
            Pseudo Genes (multiple problems)  :: 8 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2398
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639966-rid9879223.denovo.175"
                     /isolate="E20313_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            207..2069
                     /locus_tag="IWO89_002850"
     CDS             207..2069
                     /locus_tag="IWO89_002850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAQ0457538.1"
                     /translation="MQQLTKRERQLLIYLVEMSEPVTIKKLAEKENVSVRTIKYDLDD
                     IREWLNERKQVLYSKRSQGIWMNISSSDRIKLKSELMDVNRFEIFADQSLRIKRLLIM
                     LLVTKEAITGMKMANALDVSKDTIMNDLDVLENNFLKEGLTLNRQARKGFWIIGEERL
                     VRLTIEEILQKEFTDYDIYKLMSLLLNGGETEYFEMYSATATPIQEVFNQVIIRMRHL
                     LEFENLEKLNYAELLNILIRVTIATVRLRKEATIGRYQLVAEQELDKNDLSYRVMQQV
                     FDYYQLPLFEDEYHYIYSDTFDTIHQQDVMKLVENVIYKVSKAINHSFYQDPQLLTNL
                     YAHLSMRLSRKKKFVNEYNPFKEDIKQKYPELFDAIKQAVRQEISEQNILINDSFVAY
                     IALHFLVSYEREEDLRSVRAVYICSTGLGVTSLIKQKINEELNNITIVAFASVLNAQE
                     MIATKDPDLVISIFPINGIDRPFIKVHPLPTEQDLKKIQQIIKKILASSNQQERHNLN
                     LRETSINKDTLEDFSKELILKAYTIYERLLVILEAELIQEYREAFLLHVFLMVHRITF
                     DQQYMAEGRQVDQIILDKGTLVGKIEAVFAENNLGINQSEISALFSYIKEGASS"
     gene            2069..>2398
                     /locus_tag="IWO89_002851"
     CDS             2069..>2398
                     /locus_tag="IWO89_002851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAQ0457539.1"
                     /translation="MKMSDEALKIIEESQNREKLKQVIDLIEERLVEHAIVPTELQWT
                     ILINHLNEMLKRSQRKETIPVVDRDLFKEVSKEAITISDEIVRSIGNLTDDEIYVLSI
                     HFETAKNN"
BASE COUNT          865 a          353 c          466 g          714 t
ORIGIN      
        1 aaaaatttga aaaagcgttt gattatttat attacgactt agtgcaataa aaaacgcttt
       61 ttaacagtac atgtagtcgt ctttataata gataaactct gtaatattag ttaaaaagct
      121 ataagttgca cttagttaag tgcaaacatt tcatattgtc gttagtttag aaaacgctta
      181 tattagcaat gtaaggagag atgataatgc agcagctaac aaaaagagaa aggcagcttt
      241 tgatttactt ggtggaaatg tctgaaccgg taacaatcaa aaagttggct gaaaaagaaa
      301 atgttagtgt tcggacgata aaatacgatc tagatgatat aagagagtgg ctgaatgagc
      361 ggaagcaagt cctatatagc aaacgtagtc agggaatttg gatgaatatt agtagctctg
      421 atcgaatcaa attaaaaagc gaattgatgg atgttaatcg ttttgaaata tttgcagatc
      481 aaagtctccg aatcaaacgt ttactaatta tgcttttagt aacaaaagaa gctattactg
      541 gaatgaaaat ggccaacgca ttagatgtca gcaaagatac gatcatgaat gatttagatg
      601 tgctggaaaa taattttctt aaggaaggat tgaccttgaa tcgtcaggcg cgaaagggtt
      661 tttggatcat aggagaagaa cgtctagttc gattaacgat tgaagagatt cttcaaaaag
      721 agttcacgga ttatgacatt tataaattaa tgtcattgtt attgaatggc ggtgagactg
      781 aatactttga aatgtattct gctactgcta cacctatcca agaggtattt aatcaagtaa
      841 tcattcgaat gcgacactta ctggaatttg aaaatctaga gaaactgaat tacgcggaat
      901 tactaaatat tctgattcga gtgacaattg ctaccgttcg cttacgcaaa gaggctacga
      961 ttggtcgtta tcaattggtt gctgaacaag aattagacaa aaacgatctt tcttatcgcg
     1021 tcatgcagca ggtctttgat tattatcagt tgccactgtt cgaggatgaa tatcattata
     1081 tctatagtga tacattcgac acgattcacc agcaagacgt catgaaacta gttgagaatg
     1141 tgatttataa agtaagtaaa gcaatcaacc attcttttta tcaagatcca caattgttga
     1201 cgaatctgta tgctcattta tccatgcgat tatcgagaaa gaaaaaattt gtcaatgaat
     1261 ataatccttt taaagaagat atcaagcaga aatacccgga attatttgat gcgatcaagc
     1321 aagcagttcg acaagaaata agtgaacaaa atattctgat caatgattct ttcgtggcat
     1381 atattgcctt acactttttg gtttcctacg aaagagaaga agatctacga agcgttagag
     1441 ccgtctacat ttgttcaaca gggttgggtg tgactagtct gatcaagcaa aagatcaatg
     1501 aagaattaaa taatatcact atcgtggcat ttgcttcagt attgaatgcg caagaaatga
     1561 ttgctaccaa agatcctgat ttggtgatta gtatttttcc aattaatggc atcgatcgtc
     1621 ctttcatcaa agtccatcca ttacccactg aacaggactt gaaaaaaatt caacaaataa
     1681 taaaaaaaat acttgcttct tccaatcagc aggaaaggca taacttgaat cttagagaaa
     1741 cgtccatcaa taaagatact ttagaagact ttagcaagga attgattttg aaagcttata
     1801 cgatttatga acgtttactt gtcattttgg aagcagaatt gatccaagaa tatcgagaag
     1861 cttttttgct tcatgtattc ttaatggttc atcgtatcac atttgatcaa caatatatgg
     1921 cagaaggtcg tcaagtagac cagattattt tagacaaagg aacactggta ggaaaaatcg
     1981 aagcagtttt tgcagaaaat aatttaggaa tcaatcagtc agaaatcagt gcattattct
     2041 catatataaa agaaggagcg tcaagttagt gaaaatgtca gacgaggctt taaaaattat
     2101 cgaggaaagt caaaatcggg aaaagttaaa acaagtgatt gatctaattg aagagcgatt
     2161 agtagaacat gctattgtac caaccgagtt gcagtggacc attctaatta atcacttgaa
     2221 tgagatgctg aaacgttctc aaagaaagga aacgattccc gtagtcgatc gggatttgtt
     2281 caaagaagta tccaaagaag ccataactat ttctgatgaa atagttagaa gtatcggtaa
     2341 tttaaccgat gatgaaatat atgttttgtc gattcatttt gaaacagcaa aaaataat
//
LOCUS       DACEEZ010000169         2203 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20313_2017
            SAMN12639966-rid9879223.denovo.178, whole genome shotgun sequence.
ACCESSION   DACEEZ010000169 DACEEZ010000000
VERSION     DACEEZ010000169.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639966
            Sequence Read Archive: SRR10023158
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2203)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2203)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741683.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741683.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:44:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,016
            CDSs (total)                      :: 2,962
            Genes (coding)                    :: 2,889
            CDSs (with protein)               :: 2,889
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 36 of 73
            Pseudo Genes (incomplete)         :: 29 of 73
            Pseudo Genes (internal stop)      :: 18 of 73
            Pseudo Genes (multiple problems)  :: 8 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2203
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639966-rid9879223.denovo.178"
                     /isolate="E20313_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            <1..605
                     /locus_tag="IWO89_002852"
     CDS             <1..605
                     /locus_tag="IWO89_002852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HAQ0457540.1"
                     /translation="PKGTTYTWKEKVDVSTAGNKKGTVVVTYPDGSKEEVEVTISVVD
                     KKAPNKPQVDPITEGDQIVTGKTEPNAEVTVTLPDGSQHHGTADKNGNFTVKVPKLEA
                     GTKVIVTATDESGNTSEPTNVVVSSNKKDSGKNGSKGSKTDNQGSNSNQDKNRGKSQS
                     SKVFPKTGESDSNIFTISGGLILLGTLGLLGYKNRKKENE"
     gene            739..2082
                     /locus_tag="IWO89_002853"
     CDS             739..2082
                     /locus_tag="IWO89_002853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HAQ0457541.1"
                     /translation="MEKIVIIGSNHSGLAAARFLNLSDSNYEVILIDKKSEISYLECG
                     TSLFIKGEVSNLEDLFYQNRMTLNSEIKKLFLETEVDKIDIQNKKISCVKKDGTRFSQ
                     EYDKVILATGSKQIMLDIPGNNLANIFSIKNFPNAKEIFKALDDSTIKKVGIIGAGYI
                     GVEIAEAIRKRGKEVYLFDVADRVLSTYYDRSFSDKVEEILSKNGIHLCLSESPKKYL
                     GRKKIEKIVTTSGTQYSMDMVVQAIGFLSNSPIGEKELLRDVSGAYLTNEYQQTNIKD
                     IYAIGDCATTFFTSINRSSVDFSISNALRTAYIATQHINNQDFTPSGSQFTNGFSIFN
                     YNFYAAGLNLKTARLLSESIDYIEIEDFIRPAFLRKNSRVKLRISFEKNTKRIVGVQL
                     CSQEDLSGLLSMFSLAIEEQITLDKLKMLDYLFHPSFSQPYNFILKAVTGLNIET"
BASE COUNT          877 a          269 c          394 g          663 t
ORIGIN      
        1 taccaaaagg aactacttat acttggaaag aaaaagtaga tgttagtaca gctggaaaca
       61 aaaaaggtac tgtagtagta acatatccag atggatctaa agaagaagta gaagttacta
      121 tttctgtagt agataaaaaa gcaccgaata aacctcaagt tgatcctatt acagagggtg
      181 accaaattgt tactggtaaa actgaaccaa atgcagaggt gacagttaca ttaccagacg
      241 gaagtcaaca ccatgggaca gctgataaaa atggtaattt tacagttaaa gttcctaaat
      301 tagaggcagg gacaaaagtg atagtaactg caactgatga atctggtaat actagtgaac
      361 ctactaatgt agttgtttct tccaacaaaa aagacagtgg aaaaaacggt agtaaaggta
      421 gtaagactga caatcaaggt agcaacagca atcaagacaa aaatagaggc aaaagccagt
      481 cctcaaaagt ttttcctaag acgggagaga gtgacagtaa tatctttact attagcggtg
      541 gtttaatact gttaggaacg ttaggattat tagggtataa aaatcgaaaa aaagagaatg
      601 aatagaaaat caatttctta agtcttctct gagaagagga agaatctttt tcttcctctt
      661 tttgcttttt tatgatggga aataagtgtt agttaaagat gaatatactt tttatagaaa
      721 ggaaaagaat gtaattaagt ggaaaaaata gtaattattg gttcaaacca ttcagggcta
      781 gccgcagctc gatttctaaa cttaagcgat tcaaattatg aagttattct aatcgataaa
      841 aaatcagaaa ttagttactt ggaatgtggg acatcgttat ttataaaagg agaagtaagt
      901 aatttagaag atctttttta tcaaaataga atgacactaa attctgagat aaaaaagtta
      961 tttctagaga cagaagttga caaaattgat attcaaaata aaaagattag ttgtgtaaaa
     1021 aaagatggta caagattctc tcaagaatat gacaaagtta tattagctac aggttcaaag
     1081 caaattatgc tagatatacc ggggaataat ttagctaata ttttttcgat aaagaatttt
     1141 ccaaatgcaa aagagatttt taaagctttg gatgatagta ctattaaaaa agtaggaatt
     1201 attggagcag gatatattgg agttgaaatt gctgaagcca taagaaaaag agggaaagaa
     1261 gtatatcttt tcgatgttgc agatagagtg ttgtcgactt attacgaccg tagtttttca
     1321 gataaagttg aagaaatact atcaaaaaat ggaatacatc tttgtctatc agaaagtcct
     1381 aaaaaatatt taggaagaaa aaagattgaa aaaatagtca ctacaagtgg tacccaatac
     1441 tctatggata tggtcgtaca agctatagga tttctatcta atagtccaat tggggaaaag
     1501 gaactcttga gagatgttag tggagcatat ctaactaatg aataccagca aacaaatata
     1561 aaagatatat atgctattgg ggattgtgct acaacttttt ttacgtccat taatagaagt
     1621 agtgttgatt tttctatttc taatgctcta agaacagctt atattgcaac tcaacatatt
     1681 aacaatcaag attttacacc ttctggttcc caatttacaa atggcttttc tatatttaat
     1741 tacaattttt acgcagcagg tttgaattta aaaacagcac gattattaag tgagtctata
     1801 gattatatag aaatagaaga tttcattaga cctgcttttt tgagaaaaaa tagtcgtgta
     1861 aaattaagaa ttagctttga aaaaaataca aagagaatag taggtgtaca actttgtagt
     1921 caagaagatt tatctggact attatcaatg ttttctcttg ctatagaaga acaaattaca
     1981 ttagacaaat tgaaaatgtt agattattta tttcatccta gttttagcca accctataat
     2041 tttattttaa aggcagtaac aggattaaac attgaaacat aatcgttact aaatgaaatc
     2101 aagaaatata ctgtattact caattcattg aaaatattta tcataatttt taatagaaaa
     2161 cactattaaa gagaaaaaag agaggtaaat caaatgaaaa aat
//
LOCUS       DACEEZ010000170         2194 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20313_2017
            SAMN12639966-rid9879223.denovo.179, whole genome shotgun sequence.
ACCESSION   DACEEZ010000170 DACEEZ010000000
VERSION     DACEEZ010000170.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639966
            Sequence Read Archive: SRR10023158
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2194)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2194)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741683.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741683.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:44:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,016
            CDSs (total)                      :: 2,962
            Genes (coding)                    :: 2,889
            CDSs (with protein)               :: 2,889
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 36 of 73
            Pseudo Genes (incomplete)         :: 29 of 73
            Pseudo Genes (internal stop)      :: 18 of 73
            Pseudo Genes (multiple problems)  :: 8 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2194
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639966-rid9879223.denovo.179"
                     /isolate="E20313_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(<1..986)
                     /locus_tag="IWO89_002854"
     CDS             complement(<1..986)
                     /locus_tag="IWO89_002854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006701269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256-like element IS1542 family transposase"
                     /protein_id="HAQ0457542.1"
                     /translation="MTQVHFTLNNEEVQSIIEHSVKDDVSKNILTTVFNQLMENQRTE
                     YIQADDYERSESRQSQRNGYYERDFTTRVGTLELKVPRTRDGEFSPTVFERYQRNEKA
                     LLASMLEMYVSGVSTRKVSKIVEELCGKSVSKSFVSSLTEQLDPMVNEWQNRSLSGTN
                     YPYLMTDVLYIKVREDHRVLSKSCHIAIGITEGGDREIIGFMIQNEESDDTWSIFFEY
                     LKERGLKGTELIISDAHKGLVSAIRKSFTNASWQRCQVHFLRNIFSSIPKKNSKPFRE
                     AVKAIFKFTDIELARTAKNALVGEYIDQPKYTKACEILDNGFEDAFQYTVIG"
     gene            1138..1833
                     /gene="vanR"
                     /locus_tag="IWO89_002855"
     CDS             1138..1833
                     /gene="vanR"
                     /locus_tag="IWO89_002855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001280781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanA-type vancomycin resistance DNA-binding
                     response regulator VanR"
                     /protein_id="HAQ0457543.1"
                     /translation="MSDKILIVDDEHEIADLVELYLKNENYTVFKYYTAKEALECIDK
                     SEIDLAILDIMLPGTSGLTICQKIRDKHTYPIIMLTGKDTEVDKITGLTIGADDYITK
                     PFRPLELIARVKAQLRRYKKFSGVKEQNENVIVHSGLVINVNTHECYLNEKQLSLTPT
                     EFSILRILCENKGNVVSSELLFHEIWGDEYFSKSNNTITVHIRHLREKMNDTIDNPKY
                     IKTVWGVGYKIEK"
     gene            1811..>2194
                     /locus_tag="IWO89_002856"
     CDS             1811..>2194
                     /locus_tag="IWO89_002856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanA-type vancomycin resistance histidine kinase
                     VanS"
                     /protein_id="HAQ0457544.1"
                     /translation="MVIKLKNKKNDYSKLERKLYMYIVAIVVVAIVFVLYIRSMIRGK
                     LGDWILSILENKYDLNHLDAMKLYQYSIRNNIDIFIYVAIVISILILCRVMLSKFAKY
                     FDEINTGIDVLIQNEDKQIELSAEMD"
BASE COUNT          670 a          423 c          434 g          667 t
ORIGIN      
        1 tttccgataa ccgtgtattg aaaggcatct tcgaagccat tatccaatat ttcgcaggct
       61 tttgtatatt taggctggtc gatatattca ccgactaagg cattcttagc tgttcgagcg
      121 agttcaatat ccgtaaactt aaagatcgct tttactgctt ctctaaacgg ttttgaattc
      181 ttttttggaa tggaactgaa gatgtttctt aaaaaatgga cctggcatct ctgccaactt
      241 gcgttggtaa atgacttacg aatcgcagac actaggcctt tatgggcatc agaaatgatg
      301 agttctgtcc cctttaggcc gcgttctttt aagtattcaa agaagatgga ccatgtgtca
      361 tcactttctt cattttgaat catgaagcca atgatttcac ggtcgccacc ttctgttatc
      421 ccgatcgcaa tatggcagct tttagaaagc actcgatggt cctcacggac ttttatgtag
      481 agaacatcag tcatcagata aggataattc gtacctgaga gtgaacggtt ctgccattcg
      541 ttgaccatcg ggtctaactg ctcagtcagg ctagaaacaa aagatttcga tacagatttt
      601 ccacatagct cttcaacaat ctttgaaact ttacgtgtcg aaacgcccga aacatacatc
      661 tcaagcattg aagcgagcag tgccttttca tttcgctgat aacgctcaaa caccgtcggt
      721 gaaaattcac catcacgtgt tctaggcact tttaattcga gtgtacccac acgagtcgta
      781 aagtctcgct cataatagcc atttctttga ctctgacgac tttctgaacg ttcatagtca
      841 tcggcttgaa tatattctgt tcgttgattt tccatcaatt ggttgaaaac agtggttaaa
      901 atatttttag aaacatcatc ttttaccgaa tgttcaataa tactttgaac ctcttcgttg
      961 ttcagtgtaa aatgtacttg ggtcatgtaa cgtcctcctg ggtatgtttt tgtggttaaa
     1021 aacattgtac cgtaaaaggg ctgttacatg gccttttatc ttttacacaa ttatatggac
     1081 tttatcctat aatctgataa attattaagg gcaaacttat gtgaaagggt gataactatg
     1141 agcgataaaa tacttattgt ggatgatgaa catgaaattg ccgatttggt tgaattatac
     1201 ttaaaaaacg agaattatac ggttttcaaa tactataccg ccaaagaagc attggaatgt
     1261 atagacaagt ctgagattga ccttgccata ttggacatca tgcttcccgg cacaagcggc
     1321 cttactatct gtcaaaaaat aagggacaag cacacctatc cgattatcat gctgaccggg
     1381 aaagatacag aggtagataa aattacaggg ttaacaatcg gcgcggatga ttatataacg
     1441 aagccctttc gcccactgga gttaattgct cgggtaaagg cccagttgcg ccgatacaaa
     1501 aaattcagtg gagtaaagga gcagaacgaa aatgttatcg tccactccgg ccttgtcatt
     1561 aatgttaaca cccatgagtg ttatctgaac gagaagcagt tatcccttac tcccaccgag
     1621 ttttcaatac tgcgaatcct ctgtgaaaac aaggggaatg tggttagctc cgagctgcta
     1681 tttcatgaga tatggggcga cgaatatttc agcaagagca acaacaccat caccgtgcat
     1741 atccggcatt tgcgcgaaaa aatgaacgac accattgata atccgaaata tataaaaacg
     1801 gtatgggggg ttggttataa aattgaaaaa taaaaaaaac gactattcca aactagaacg
     1861 aaaactttac atgtatatcg ttgcaattgt tgtggtagca attgtattcg tgttgtatat
     1921 tcgttcaatg atccgaggga aacttgggga ttggatctta agtattttgg aaaacaaata
     1981 tgacttaaat cacctggacg cgatgaaatt atatcaatat tccatacgga acaatataga
     2041 tatctttatt tatgtggcga ttgtcattag tattcttatt ctatgtcgcg tcatgctttc
     2101 aaaattcgca aaatactttg acgagataaa taccggcatt gatgtactta ttcagaacga
     2161 agataaacaa attgagcttt ctgcggaaat ggat
//
LOCUS       DACEEZ010000171         2103 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20313_2017
            SAMN12639966-rid9879223.denovo.180, whole genome shotgun sequence.
ACCESSION   DACEEZ010000171 DACEEZ010000000
VERSION     DACEEZ010000171.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639966
            Sequence Read Archive: SRR10023158
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2103)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2103)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741683.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741683.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:44:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,016
            CDSs (total)                      :: 2,962
            Genes (coding)                    :: 2,889
            CDSs (with protein)               :: 2,889
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 36 of 73
            Pseudo Genes (incomplete)         :: 29 of 73
            Pseudo Genes (internal stop)      :: 18 of 73
            Pseudo Genes (multiple problems)  :: 8 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2103
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639966-rid9879223.denovo.180"
                     /isolate="E20313_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            169..1539
                     /locus_tag="IWO89_002857"
     CDS             169..1539
                     /locus_tag="IWO89_002857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4357 domain-containing protein"
                     /protein_id="HAQ0457545.1"
                     /translation="MMDKSNEEQTTLYTKYWRRLEEVFDNSKGMEDFFRYYLAAITGE
                     YSAKHILYQAFKDYWHKERDVSSDAELLQKLVRYAGYFARLYYNKPEGKYSEVLSDFQ
                     SMESMMPAPFVLGLSEWYYHDQFITEDQYIDAIKVVNDYQLRRYFNGDDTSRVSKAFP
                     SYLKSVRKYAKANGYENIVDIVIYVLVTRNQSNNMALPSDKALKSNLLNANAYAMRLA
                     RWLLEKIENRENSATLDMSNLSIEHIMPQTSTSYWEEKAGTSGEEYTGLVNTIGNLTL
                     VTNPDNSAAGNKDFETKKKIFEDTLHIRMNKDLYELTEWTSSDISARSEALINELITM
                     YPYLRSSGDYEHDGNREIFLEAQGIKATGYLNEDETVIIHSGSEIYSKIKDIASDSLD
                     ETRQELLDNGIIEETIGGLQFVQDYTASSVSNAAALLLGGSRNGWDYWKYDNGISIND
                     SLRNKK"
     gene            1560..1844
                     /locus_tag="IWO89_002858"
     CDS             1560..1844
                     /locus_tag="IWO89_002858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0457546.1"
                     /translation="MDKNKLRYAILKLRDEKKNPFIELKGQVPEEDILEQTKFLSRNG
                     LLESMVWADNTVHIWGSVSLEGEKYLVENSGLAKAYALAKEVKNWIPFWG"
BASE COUNT          730 a          308 c          444 g          621 t
ORIGIN      
        1 ttgatttacc aacatgttta gacattttcc tctaggaaaa aattaactag caccacgcgt
       61 attgtttata ttaaattaga tgaccgtgat aatgcacagc aaatttttga aagtatcaat
      121 tctaccggtg aaagattgac tgcctcggat ttgattcgaa acttcatcat gatggataaa
      181 tctaatgaag agcagactac gctttatact aaatattggc gtaggttgga agaagtcttt
      241 gataattcta aaggaatgga agactttttc agatactacc ttgctgcaat aactggggag
      301 tattcagcaa agcatattct ttatcaagca ttcaaagact attggcataa agagcgcgat
      361 gtatcaagtg atgccgaatt gctccaaaaa ttagtgcgat atgctggtta ttttgcgaga
      421 ctttactata acaaacctga aggaaaatac tcagaagtat tgagcgattt ccaaagtatg
      481 gaaagtatga tgcctgcacc ttttgttttg ggattatctg aatggtacta tcatgaccaa
      541 tttatcactg aagaccaata tattgatgcg attaaggtag tgaatgatta tcagcttagg
      601 agatatttta atggggatga tacaagcaga gtgtcaaaag catttccttc atatttgaaa
      661 agtgttagga agtacgctaa agctaatgga tatgaaaata tcgttgatat tgtaatttat
      721 gttcttgtta ctagaaatca atcaaataat atggcacttc catctgacaa ggcattgaaa
      781 tctaatctat tgaatgccaa tgcttatgcg atgagattgg cccgttggtt acttgaaaaa
      841 atagagaatc gagaaaattc agcaacactt gatatgagta acttgtccat tgaacacatc
      901 atgccacaga ctagtacttc atattgggaa gagaaggctg gtacatcggg ggaagaatac
      961 acaggattag taaataccat tggtaattta acactcgtta caaatcctga taattcggcc
     1021 gcgggaaata aagactttga gaccaaaaag aaaatctttg aagataccct tcatattcgt
     1081 atgaataaag atttgtacga attgactgag tggacatcat cggatatttc tgccagaagt
     1141 gaagctctaa tcaatgaact gattactatg tatccctatt tgagatcgtc gggcgactat
     1201 gaacacgatg ggaataggga aattttctta gaggcgcaag gaataaaagc aactgggtat
     1261 ttgaacgaag acgagaccgt gattatacat tcaggttcag aaatttattc taagattaaa
     1321 gatattgcat ctgattcgct agatgaaact cgccaagagc tgttggataa tggaatcatt
     1381 gaagaaacaa ttgggggact acaatttgtt caagattaca ctgcctcatc tgtttcgaat
     1441 gcagcagctc tgctattagg gggtagccgg aatggttggg actactggaa atatgataac
     1501 ggaataagta ttaatgattc gctaagaaat aagaaataga gcatagaggt ggtgttaggt
     1561 tggataagaa caaactaaga tatgcaattc ttaaattgcg tgatgaaaaa aagaatcctt
     1621 ttattgagct taaagggcaa gttccagaag aagatatttt agaacaaact aaatttcttt
     1681 cgcgcaatgg cttactagaa tccatggttt gggcggataa cactgttcat atctggggga
     1741 gtgtttctct ggaaggggaa aaatatttag ttgaaaactc aggtcttgct aaggcgtatg
     1801 cattggcaaa agaagttaaa aattggattc cattttgggg ctaagataag tttttaaacg
     1861 gagaaggtag caaatcataa ttggtttgtt gccttttttg attgcccaaa aagaaaaatg
     1921 acaaagtata gagataaatg acatagtaca cgggaaaaat gacattgtat caccgacaac
     1981 gtctaaatag atgttgatat atatacgttc cgtacaaata attataaaaa tttaaatgat
     2041 ttgagtagaa gtatcagtga atatctcaag gggaatagct atctcaagat tcgattttca
     2101 cat
//
LOCUS       DACEEZ010000172         2021 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20313_2017
            SAMN12639966-rid9879223.denovo.181, whole genome shotgun sequence.
ACCESSION   DACEEZ010000172 DACEEZ010000000
VERSION     DACEEZ010000172.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639966
            Sequence Read Archive: SRR10023158
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2021)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2021)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741683.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741683.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:44:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,016
            CDSs (total)                      :: 2,962
            Genes (coding)                    :: 2,889
            CDSs (with protein)               :: 2,889
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 36 of 73
            Pseudo Genes (incomplete)         :: 29 of 73
            Pseudo Genes (internal stop)      :: 18 of 73
            Pseudo Genes (multiple problems)  :: 8 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2021
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639966-rid9879223.denovo.181"
                     /isolate="E20313_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            273..839
                     /gene="amaP"
                     /locus_tag="IWO89_002859"
     CDS             273..839
                     /gene="amaP"
                     /locus_tag="IWO89_002859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline shock response membrane anchor protein
                     AmaP"
                     /protein_id="HAQ0457547.1"
                     /translation="MNKGLKIIGVIMSLLLLSVFLAIALINAPYIMPEQLERFRFFTL
                     TNYYMQQYIFWAAVVFAVLAIIILLVVLFYPKSRGTFVMKREDGKLTIDKKAIEGLVR
                     SHLHEEEFIHSPKVRIRSTKNRIHIHVNGDLKRTSSLVGKTGVLMQDIEEEVTKVLGT
                     KETVKVAVTYSGYEEQEDTRDYKHSRVE"
     gene            852..1040
                     /locus_tag="IWO89_002860"
     CDS             852..1040
                     /locus_tag="IWO89_002860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2273 domain-containing protein"
                     /protein_id="HAQ0457548.1"
                     /translation="MKELFSAYKLPIIFGGLGLLLAVLWLTIGFFKTLLIIIMTAIGA
                     GIGFYLKQSGIVDQFFKS"
     gene            1053..1610
                     /locus_tag="IWO89_002861"
     CDS             1053..1610
                     /locus_tag="IWO89_002861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HAQ0457549.1"
                     /translation="MENKNMNTTNGSVLANEKGENRMMNGELTYEDKVIQKIIGYALK
                     DVDGLLTLDGGFFSNMAEKLVNTDNVTAGINTEVGKKQVAVDLDVIVEYGKDIEDIYN
                     QIKELISTEVNNMTHLDVIEVNVNVADIKTQEEYQKDQETVQDKVTEAAKSTGQFASR
                     QTDKAKSAVGKGAQKVKENNEPRVQ"
     gene            1642..1884
                     /locus_tag="IWO89_002862"
     CDS             1642..1884
                     /locus_tag="IWO89_002862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HAQ0457550.1"
                     /translation="MGFIWALIVGGIIGAIAGAITNKGSSMGIIANIIAGLVGSAIGQ
                     ALLGTWGPTIAGMAIIPSLIGAIILVLVVSFFARKL"
BASE COUNT          743 a          309 c          407 g          562 t
ORIGIN      
        1 aataaataga atcttgtaat aataactgag cataatcttt gcaattttta atcttgaata
       61 atatccttta agtatagcca gcaatcaaaa gtaaatagag aataagtaag caattgttat
      121 ttcagtatca gactggcaat aaaacgccgt tatgatccag caatgtttgg tagcgcttta
      181 tttactgatg ctatgctaac tgttcgctat ctatactgtg attgtcggta gagttattta
      241 cagggtaaaa ataagtttat aggaggtgga aaatgaataa ggggttaaaa ataatcggtg
      301 ttatcatgag tttactgcta ctttcagtat ttctagcgat tgcgttgatc aatgcgccgt
      361 atattatgcc tgaacaattg gaacgatttc gcttctttac tttaacgaat tactacatgc
      421 aacaatatat tttctgggca gctgtagtat ttgccgttct tgctattatc atcttgctag
      481 tcgttctttt ctatccaaag tcaagaggaa ctttcgttat gaaacgggaa gatggcaaac
      541 taacaatcga caaaaaagcg atcgaagggt tggtacgttc ccatttacac gaagaagagt
      601 tcattcattc acctaaagta cggatacgtt caacgaaaaa tcgaatccat atccatgtca
      661 acggagactt gaaacgaact tcttcattag tagggaaaac aggtgtattg atgcaagata
      721 tcgaagaaga agtaactaaa gtactcggaa caaaagaaac ggtcaaagtg gcagtcacat
      781 acagcggtta tgaagaacaa gaagatacaa gagactacaa gcactctcga gtggaatagg
      841 agggccaaat aatgaaagaa ctattttcag catacaagct ccctattatc tttgggggtc
      901 ttggactact gctagctgtt ttatggttaa cgattggatt tttcaagaca ttgttgatca
      961 taatcatgac agcaattggc gcaggaatcg gtttttattt gaaacagtca ggaatagttg
     1021 atcaattttt taaatcatag gaggaaacaa agatggaaaa caaaaacatg aatacaacaa
     1081 atggatcagt tttggcaaat gaaaaaggag aaaaccgcat gatgaatggt gaattgactt
     1141 atgaagacaa agtcatccaa aaaatcatcg gttatgcatt gaaagatgta gatggtctat
     1201 tgacattaga tggcggattt ttctcaaata tggctgaaaa attagtcaac acagataacg
     1261 taacagcagg tatcaataca gaagtaggta agaaacaagt agctgtggat ctagatgtga
     1321 tcgtagaata cggtaaagac atcgaagata tttacaacca aatcaaagaa ttgatcagta
     1381 ctgaagtaaa caatatgaca catttagatg ttatcgaagt gaatgtgaat gttgcagaca
     1441 tcaaaacaca agaagaatac caaaaagatc aagaaacagt acaagacaaa gtaacagaag
     1501 cagcaaaatc cacaggacaa tttgcttcac gccaaacaga taaagcaaaa tctgctgtag
     1561 gcaaaggtgc acaaaaagtc aaagaaaata acgaaccacg tgtacaataa gaagtaagaa
     1621 gtcagttagg aggaatttaa aatgggattt atttgggcat taatcgtcgg cggtatcatt
     1681 ggagcaattg caggagcaat cacgaataaa ggatcatcaa tggggatcat cgcaaacatc
     1741 attgctggtt tagtaggttc agcaattgga caagctttac taggaacttg gggaccaaca
     1801 attgcaggaa tggctatcat tccatcattg atcggtgcaa tcattttagt attagtcgtt
     1861 tcattctttg cacgtaaatt ataagcaaat tgaagaaaaa cttatgttga aatgaagaac
     1921 aaatttgaga accgtagcga cactcgctgc ggttcttttt tatatatttt atatgaagta
     1981 ttctttattc cttatgaaaa aatatcttta aaaataaaaa a
//
LOCUS       DACEEZ010000173         1987 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20313_2017
            SAMN12639966-rid9879223.denovo.182, whole genome shotgun sequence.
ACCESSION   DACEEZ010000173 DACEEZ010000000
VERSION     DACEEZ010000173.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639966
            Sequence Read Archive: SRR10023158
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1987)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1987)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741683.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741683.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:44:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,016
            CDSs (total)                      :: 2,962
            Genes (coding)                    :: 2,889
            CDSs (with protein)               :: 2,889
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 36 of 73
            Pseudo Genes (incomplete)         :: 29 of 73
            Pseudo Genes (internal stop)      :: 18 of 73
            Pseudo Genes (multiple problems)  :: 8 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1987
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639966-rid9879223.denovo.182"
                     /isolate="E20313_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            <1..159
                     /locus_tag="IWO89_002863"
     CDS             <1..159
                     /locus_tag="IWO89_002863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0457551.1"
                     /translation="KEVDHAEYTFAALCRIHHTEKHKIGVINFKNKYQIKGIKLNQKT
                     IKKLNIGG"
     gene            162..911
                     /locus_tag="IWO89_002864"
     CDS             162..911
                     /locus_tag="IWO89_002864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAQ0457552.1"
                     /translation="MTEHRSYYAIIPANVRYDKRLKPNTKLLYGEITALCNERGFCWA
                     GNEYFADLYGVNKETISRWVSDLIKFGYLNREIIYKEGTNQIINRYLRINQYPIDEKR
                     NTPIDEKVKDNNTSINNTFNNTKEYIRELPPSKKSKAKPVRHKYGEYKNVLLSDEQME
                     KLKTEFPNDYQERIERLSEYCESSGKTYKNYLATIRSWARKEKSEPKNASSGYKRTGR
                     REKLPEWAIDQEAYLKKKALERANRQSKAPF"
     gene            923..1192
                     /locus_tag="IWO89_002865"
     CDS             923..1192
                     /locus_tag="IWO89_002865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0457553.1"
                     /translation="MKIDYLELINEIANYKKGEELDVLRDVYDQLEEAGIEGIKNDRS
                     SWSKLRYYFALYIDTTQLRNLAYTKLLFVDCVKGLQKHLNELEQV"
     gene            1354..1656
                     /locus_tag="IWO89_002866"
     CDS             1354..1656
                     /locus_tag="IWO89_002866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0457554.1"
                     /translation="MDKKAAMQRIIELTYSEDWQNDKEAASEVMRLGRAMWADKSNKP
                     RPRKIAIWHGDKLLVIGTAEQLASLTGLHEKIVRKRARCGYTDAKKRTFRYVEESS"
     gene            1653..1814
                     /locus_tag="IWO89_002867"
     CDS             1653..1814
                     /locus_tag="IWO89_002867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin"
                     /protein_id="HAQ0457555.1"
                     /translation="MTTEEVIQMRIRSLQREIDDLERTKAVMVNETARKAIDLHIENL
                     RREIHRLEE"
     gene            1817..>1987
                     /locus_tag="IWO89_002868"
     CDS             1817..>1987
                     /locus_tag="IWO89_002868"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0457556.1"
                     /translation="MDKKAAMKRIAELTKSESWQEDKEIVAEVQKLGKSMWTEKPKRR
                     TPRKIAIWHDDRI"
BASE COUNT          782 a          319 c          450 g          436 t
ORIGIN      
        1 aaagaagttg atcatgcaga gtacacattt gcagcactct gcagaatcca tcacacagag
       61 aagcacaaaa taggtgtgat taatttcaaa aataaatatc aaatcaaagg gatcaaatta
      121 aaccagaaaa caatcaaaaa gttaaatatt ggagggtaaa aatgacagaa catcgaagtt
      181 attatgcgat tataccagcc aacgtaaggt acgacaaaag acttaaacca aatactaagt
      241 tgttatacgg agagataacg gccttgtgta atgaaagagg cttttgttgg gcaggcaatg
      301 agtactttgc agatttatat ggtgtgaata aagagaccat atcgcgatgg gtaagtgatt
      361 tgattaagtt tggatacttg aatcgggaaa tcatttacaa agagggtacc aatcaaataa
      421 tcaataggta cctacgaatt aatcaatacc ctattgacga aaaacgcaat acccctattg
      481 acgaaaaagt caaagataat aatacatcta ttaataatac atttaataat acaaaagaat
      541 atataagaga gttaccgcct tcgaaaaaat cgaaggctaa gcccgtccgt cataaatacg
      601 gagagtataa aaatgttctt ttgtcagatg agcagatgga gaaactcaaa acagaattcc
      661 ctaatgatta ccaagagcga atcgaacgac tgtcagagta ttgtgaatca tctggtaaga
      721 cttataaaaa ctatttggca actattcgaa gttgggcaag gaaagaaaaa agtgaaccta
      781 agaacgcaag cagtggatac aagcgcacag gaagacgaga gaagcttcct gaatgggcaa
      841 tcgaccaaga agcctatctc aagaaaaaag cgctagaacg agctaataga caatcaaaag
      901 caccattcta agaggtggaa aaatgaagat cgattatcta gaactaatta atgaaatagc
      961 gaattataaa aagggcgagg aattagacgt cctgagagac gtatatgatc aactcgaaga
     1021 agctggaatt gaaggaatta agaatgatcg ttcgagttgg agtaagctca gatactattt
     1081 cgcactttat atcgatacaa cacaattaag aaatttagca tataccaaat tactatttgt
     1141 tgattgtgtt aaaggcttgc aaaaacatct taatgaactt gagcaggtgt aatcagatgg
     1201 acctaaagac atttacagca cagattgaac taatgcatca agaagcttta agacaaagtg
     1261 tatcgtacga agacaagtgg ctcaacacgt tccatggcgg acgtgagagc gcacttgatc
     1321 aagtactcaa attactgaaa ggagaatgtc gggatggata agaaagcggc aatgcagcga
     1381 attatcgaat tgacttattc agaagattgg caaaatgaca aagaagctgc ttcagaagtg
     1441 atgaggcttg gaagagcgat gtgggcagac aagagcaaca agccaagacc acgaaaaatc
     1501 gcaatttggc acggtgacaa acttctagtg atagggacag ctgaacagtt agcaagtctc
     1561 acaggcttgc acgagaaaat cgtgaggaaa agagcaaggt gtggctacac agacgctaag
     1621 aagagaacgt ttagatacgt ggaggaatca tcatgacaac agaagaagtg attcaaatgc
     1681 gtattcgaag ccttcagcgt gagattgacg atctggaacg aacaaaggca gtgatggtca
     1741 atgaaacggc gagaaaggca atcgatttgc acatagagaa tttaagaagg gaaatccatc
     1801 gattggagga atgagcgtgg ataagaaagc agcaatgaaa agaattgctg aattaaccaa
     1861 gtcagaatct tggcaagaag acaaagaaat agttgcagaa gtccaaaagc tcggtaaatc
     1921 aatgtggact gaaaagccca aacggagaac gccgagaaaa attgcaatct ggcatgatga
     1981 ccgaatt
//
LOCUS       DACEEZ010000174         1932 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20313_2017
            SAMN12639966-rid9879223.denovo.183, whole genome shotgun sequence.
ACCESSION   DACEEZ010000174 DACEEZ010000000
VERSION     DACEEZ010000174.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639966
            Sequence Read Archive: SRR10023158
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1932)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1932)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741683.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741683.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:44:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,016
            CDSs (total)                      :: 2,962
            Genes (coding)                    :: 2,889
            CDSs (with protein)               :: 2,889
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 36 of 73
            Pseudo Genes (incomplete)         :: 29 of 73
            Pseudo Genes (internal stop)      :: 18 of 73
            Pseudo Genes (multiple problems)  :: 8 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1932
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639966-rid9879223.denovo.183"
                     /isolate="E20313_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            202..1167
                     /locus_tag="IWO89_002869"
     CDS             202..1167
                     /locus_tag="IWO89_002869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
                     /protein_id="HAQ0457557.1"
                     /translation="MSKNGIIVNEWENLTALSNRRLNVQNLNTPLKLQWSIDRITIVG
                     KLKENIYYHTQNDVLILDFEQLMRVNEGNGYLKAVGNNGWQLLDQYEENIAYIEILKW
                     QDGKGRIDFNPNKIGHFLASSMKNFIHDLFLEPHFSRADIACDIIDVPDEFITQYRVV
                     DPVSFKPIYGRSGKLETAYWGSRASERQIRLYNKKLEQETKRKVVPKEVETWWRLEMQ
                     LRRGKATDWHNMVQESLNSFASLHFLPIDTTPTDRIMLAGLMSDQNFWSLLSRNSKYK
                     YRELLKEESQNDELTNHLRETFAESADNLKQELDTWLLGLDVTEK"
     gene            1367..1570
                     /locus_tag="IWO89_002870"
     CDS             1367..1570
                     /locus_tag="IWO89_002870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0457558.1"
                     /translation="MSQLGKCYSLSKSNRKVYTLFISDSAKEGFNLGFSISFKKGTKN
                     TSISHNNRELTDSQKINSLSGRK"
BASE COUNT          640 a          288 c          436 g          568 t
ORIGIN      
        1 aaaaaaatag tcgagcagtg ttgacgcact ctcgaccttt acggaaacta atcacactag
       61 ctccgtaact tcttaatcat tatacgaata tttttagatt tcttcaagac tagagatttt
      121 ttggtaatga atttttaaga gttacgtgaa cttttgaaaa ttctttccag aagtctcttt
      181 ttctttgagg aggaacaaaa tttgagtaaa aatggcataa tcgtaaatga gtgggaaaat
      241 ttaacagccc tatctaacag gcggttaaat gttcaaaatt tgaacacccc cctaaaatta
      301 cagtggtcaa ttgaccgaat tactatcgtt ggaaaactga aggaaaatat ttattatcac
      361 actcaaaatg atgtgttgat tttagatttt gagcaattaa tgcgggtgaa tgaaggcaat
      421 ggctatttaa aagcggttgg gaataacggc tggcaattat tagaccaata cgaagaaaat
      481 attgcttata ttgagatttt gaaatggcaa gatggcaaag ggcggattga ttttaacccg
      541 aataagattg gtcatttttt agctagttca atgaaaaatt tcattcatga tttatttcta
      601 gaaccacatt tctcacgtgc ggatattgct tgcgatatta tcgatgtgcc tgatgagttt
      661 attacacaat atcgagtggt tgatcctgtt tctttcaagc caatttatgg gcggtctggg
      721 aagctggaaa cagcttattg gggcagtcgt gccagcgaac gacaaattcg gctatacaat
      781 aaaaaattgg aacaagaaac caaacgaaaa gttgtaccaa aggaagttga gacttggtgg
      841 cggttagaaa tgcaactacg gcgtggcaag gcaaccgatt ggcataatat ggttcaagaa
      901 agtttgaata gttttgcgag cctgcatttt ttgccgattg atacaacacc tacagataga
      961 ataatgctcg ctggtttaat gtctgatcaa aatttctggt ctcttttgtc gaggaattca
     1021 aaatataaat atcgggaatt gctgaaagaa gaaagtcaaa atgatgaatt gacgaatcat
     1081 ttgcgagaaa cgtttgcaga atcggcagac aatttgaaac aagaattaga cacgtggctt
     1141 ttgggtttgg acgtgacaga gaagtgatac cgaataagaa aagtcagatg agtgagagta
     1201 aatttacgtc aatgtttaat gacgtgagtt tacgataagc gattattttg cgtgaagcaa
     1261 aataaagcgt aaaaggagtt gagagcgtga cacgctttcg gctccttttt tcgttgtcac
     1321 ttgtgacagc gaataccctc ggagagcatt tggaattgtg acagccatgt cacaattagg
     1381 aaaatgttac tctttgtcaa agagtaacag aaaggtttac actcttttta ttagcgacag
     1441 cgcgaaggag gggtttaact tggggtttag tatttcgttc aaaaagggaa caaaaaatac
     1501 aagcatttct cataataatc gtgagttaac tgatagccaa aaaattaata gtttgtctgg
     1561 tagaaaataa ttagcagatt tacaagtggg aaataaaaag ctgaaagaag aaaattgcat
     1621 ttcaaactac attttctaca ataactctaa aatggcatat tttgcgttct gagcggtttt
     1681 agcgtttatt tcgtttagtt atcggcacaa tcggtgaaac acgttagagg agcgaaaatg
     1741 gagcctgtaa ggcgactggt agcggaacgg tcgtgttaga ttgtgtagcc gatagctgaa
     1801 cgaaataaat gctggcagcg ccattctatt tcggcaggag gaggctcaag gggttcgggg
     1861 aatgaaattc cctgaggtct ttttattttt tctagaggag tgctataatc aaagaaaatg
     1921 agaaaaaaga gt
//
LOCUS       DACEEZ010000175         1796 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20313_2017
            SAMN12639966-rid9879223.denovo.184, whole genome shotgun sequence.
ACCESSION   DACEEZ010000175 DACEEZ010000000
VERSION     DACEEZ010000175.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639966
            Sequence Read Archive: SRR10023158
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1796)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1796)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741683.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741683.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:44:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,016
            CDSs (total)                      :: 2,962
            Genes (coding)                    :: 2,889
            CDSs (with protein)               :: 2,889
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 36 of 73
            Pseudo Genes (incomplete)         :: 29 of 73
            Pseudo Genes (internal stop)      :: 18 of 73
            Pseudo Genes (multiple problems)  :: 8 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1796
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639966-rid9879223.denovo.184"
                     /isolate="E20313_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            29..1381
                     /gene="brnQ"
                     /locus_tag="IWO89_002871"
     CDS             29..1381
                     /gene="brnQ"
                     /locus_tag="IWO89_002871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transport system II
                     carrier protein"
                     /protein_id="HAQ0457559.1"
                     /translation="MTKKLSFKDYLFIGSMLFGLFFGAGNLIFPVHLGQEAGAATFWA
                     NLGFLVTGIGLPFLGVIAIGVSKSSGVYELAKRINQTYAMVFTILLYLVIGPFFALPR
                     LATTSFEIGLAPFIDPSRQTLYLAIFSIVFFVLAWWFSRKPTKILDYVGKFLNPAFLV
                     LLGALLLLAFLNPLGSVNQAPIQPNYQEHAFFTGFTQGYNTLDALAALAFGIIIVTTI
                     QNMGVTKPAEIAKDTIKSGAISIILMGIIYTLLAYLGAMSLGSFALSENGGITLAQIA
                     DHYLGTYGSILLAFIVILACLKTGIGLITAFAETFTDLFPQRNYAFFVALASILPCLA
                     ANVGLTNIIQFSLPVLMFIYPLAMTLILLVLVGPLFKQRTAVYRMTTYFTLIASIFDG
                     LNACPETIKQTPIVQNLLYVAESYLPFFKLGMGWIVPAVIGFVIGLIWSFAKKEEVAD
                     "
BASE COUNT          503 a          309 c          353 g          631 t
ORIGIN      
        1 ttttaaaaaa accgtaaagg agttatgtat gacaaaaaaa ttatcattta aagactatct
       61 ttttataggc tccatgcttt tcggtctatt ttttggtgct ggaaatctta tctttcccgt
      121 tcatttgggt caagaagcag gcgctgctac cttttgggcg aatctaggtt tcttggttac
      181 cggtatcggt cttcccttct taggggtgat tgccattggt gtatcgaaaa gctcaggtgt
      241 atatgaatta gcaaaacgga taaatcaaac atatgcgatg gtttttacca tcttgcttta
      301 cttagttatc ggaccgtttt ttgctttacc tcgccttgca accacatctt tcgagattgg
      361 tttagcacca tttatcgatc ctagcagaca gacgctatat ctagcaattt tcagtattgt
      421 attttttgtg ctggcttggt ggttttcaag aaaaccgaca aagatattag attatgttgg
      481 aaagttcctg aatcctgcat ttttggtttt attaggtgct ttgcttctcc ttgctttttt
      541 gaatccacta ggttctgtaa atcaagcgcc tattcagcca aattatcaag agcatgcttt
      601 tttcacaggg tttactcaag gatataacac attagatgcg cttgctgctt tagcatttgg
      661 gatcatcatt gtcacaacaa tccaaaacat gggtgtaaca aaacctgctg aaatcgctaa
      721 agacacgatc aaatctggtg cgatcagcat tatattgatg ggaatcattt atactttgct
      781 cgcttattta ggtgctatga gcttaggtag ctttgctcta agtgaaaatg gcgggatcac
      841 gctggctcag attgctgatc attatttagg tacttatggc agtatcttat tagcatttat
      901 cgttatatta gcttgcttga agaccggtat tggtttgatt acagcttttg cagaaacatt
      961 cacagatttg tttccgcaaa gaaattacgc attctttgtt gcacttgcta gtatccttcc
     1021 ttgtttagcg gcaaacgtag gcttgacgaa tatcattcaa ttttcgttgc ctgttttgat
     1081 gttcatctat ccattagcaa tgacactgat tttactagtg ttagttggtc ctttgtttaa
     1141 gcagcgtacg gctgtttatc gaatgactac ttattttaca ttgattgctt cgatttttga
     1201 tgggttgaac gcttgcccag aaactatcaa acaaacacct attgttcaaa atcttttata
     1261 tgttgcggaa agctacttgc cgtttttcaa attggggatg gggtggatcg taccagcagt
     1321 tatcggtttt gttatcggac tgatttggag tttcgctaag aaagaagaag tagcagattg
     1381 ataaaagagg ttgtgaaaga gacgttcagc cttgagcaat aaaaagtaat tttgaaaaat
     1441 ggcttctcac atttttgcat aaatttgact taataccgag aaactgactt ttgaacaccg
     1501 tttattgaga ggtcgtgaaa aagccgctta gtttcaagta ttaagaaata ttttgtgaaa
     1561 atggcttctc acatttttgc ataaatttga cttaataccg agaaactggc ttttgaacac
     1621 cgtttattga gcgtctataa actgacctga gtattagaga aacaaaacgg caaagtagct
     1681 ccatatactt tgccgttttg ttttgaacta ataccgaaga gaagctgatc aaacaacatt
     1741 tcttttataa tatagatttt agacatatat atgatataaa aatagttttc gcaaaa
//
LOCUS       DACEEZ010000176         1774 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20313_2017
            SAMN12639966-rid9879223.denovo.186, whole genome shotgun sequence.
ACCESSION   DACEEZ010000176 DACEEZ010000000
VERSION     DACEEZ010000176.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639966
            Sequence Read Archive: SRR10023158
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1774)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1774)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741683.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741683.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:44:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,016
            CDSs (total)                      :: 2,962
            Genes (coding)                    :: 2,889
            CDSs (with protein)               :: 2,889
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 36 of 73
            Pseudo Genes (incomplete)         :: 29 of 73
            Pseudo Genes (internal stop)      :: 18 of 73
            Pseudo Genes (multiple problems)  :: 8 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1774
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639966-rid9879223.denovo.186"
                     /isolate="E20313_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            390..>1774
                     /locus_tag="IWO89_002872"
     CDS             390..>1774
                     /locus_tag="IWO89_002872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HAQ0457560.1"
                     /translation="MTQLKKLCQNRLAIVLTAVFFFWLKTIIAYYADFSLGVEGTIQY
                     FILWINPIATTLLFFGLSLYIKKPKPALAILLIIDILNTLLLYLNIIFYREFTDFITV
                     KSVLGFSKVSQGLSGSSFSLMKPHDVIYWLDIAVFIGLLVWLKVKKIPIKSNPVGKPM
                     AIAVTCLSGLIFSGNLALSEANRPQLLQRTFDRSYIVKYLGIDAYTIYDGIKTGMTSS
                     VRAHASSNGIDEVLDYTKKHYAEPNPETFGIAKGKNVIVLHLESFQQFLINMKVDGQE
                     VTPFLNSIFQNQATISFDNFFHEVGQGKTSDAENMLETGTFGLPQGSLFTELGSDNVF
                     QAAPAILGQKQGYTSAVFHGNVASFWNRDHVYKNLGYDNFFDRSYFDESDETLGYGIL
                     DKDLFRESAQYLEHLQQPFYTKFLSVTNHTPYYTDDKNFDFPSLNTGNSTVDNYVRTA
                     HYLDQSLEQFFT"
BASE COUNT          549 a          313 c          356 g          556 t
ORIGIN      
        1 caatttcaaa aaacagcttc tcatgtttta taaatattgt gacttgtggc cgaggagttg
       61 gcaggagaac accgtttaag gaagaagttg tgaaaaagct gtcctgcatc aagaaataag
      121 gagctgcgac aagaattttg tcacagcctc tttttttgta cgaataccga gaaactggct
      181 tttgaatacc gtggagtgaa gcggtcacca caagactttt atctcttcat tagtttaatc
      241 gcttacatcg cagcggtttt atgctgtgaa aagtggacgg aataatcttt agaaagaata
      301 aaaaaatctt aagataacaa cttttttagg aaattttcat ttttcaggag caaaataaga
      361 aaaaatgtga atagagagaa gtgatatacg tgacacaact taaaaaattg tgccaaaatc
      421 gtttagcaat cgttttgaca gctgttttct ttttctggct gaagacgatc attgcctact
      481 atgcagattt ttcattaggt gtggaaggta cgatccagta ctttatcttg tggatcaacc
      541 caattgcgac taccttattg ttttttggcc tttctttgta tattaaaaag ccaaagccag
      601 cgttagctat tcttttgatc attgatattt taaatacgct tttgttgtac ttgaatatta
      661 ttttttatcg ggaattcaca gatttcatta cagtcaaatc tgtattaggc ttttcgaaag
      721 tgtcgcaagg attgtcagga agctcctttt ctttgatgaa gccccatgat gtcatctact
      781 ggctggatat cgctgtgttc attgggctac tggtttggtt gaaggtcaag aaaataccta
      841 tcaaaagcaa ccctgtcggc aaaccgatgg ctatcgctgt tacctgtctt tcagggctta
      901 ttttttcagg aaatctagcg ctgagcgaag ccaaccgtcc acaattgctg cagcgaacat
      961 ttgaccgttc atatattgtc aaatatctag gaatcgatgc gtatactatt tatgacggaa
     1021 tcaaaacagg aatgactagc agtgttcgcg cgcatgcaag cagtaatggg attgatgaag
     1081 ttttagatta tacaaagaaa cattatgcag agccaaatcc cgaaacattt gggatcgcta
     1141 aagggaaaaa tgtgatcgtt ctccatttag aaagtttcca gcagtttttg attaatatga
     1201 aggtagacgg acaagaagtc acaccgtttt taaatagtat tttccagaat caagcaacga
     1261 tcagctttga caatttcttt catgaagtag gacagggaaa gacaagtgat gctgaaaata
     1321 tgttggaaac tggcacattt ggtttacctc aaggctcatt attcacagaa cttgggtcag
     1381 acaatgtctt tcaagccgca ccagcgatcc ttggacaaaa gcaaggctat acaagcgctg
     1441 ttttccatgg gaatgtagct agtttttgga atagagatca tgtatataaa aacttaggtt
     1501 atgacaattt ctttgatcga tcttattttg atgaatccga cgaaacgtta ggttatggga
     1561 tattagataa agatttattc agagaatctg cccaatatct tgagcatcta caacagccgt
     1621 tttacacgaa atttctttcc gtaaccaacc acacgcctta ttatacggat gacaagaatt
     1681 tcgactttcc atctttgaat actggcaata gcacagtaga taattatgtt cgaacggcac
     1741 attatctgga tcaatcattg gaacaatttt ttac
//
LOCUS       DACEEZ010000177         1753 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20313_2017
            SAMN12639966-rid9879223.denovo.187, whole genome shotgun sequence.
ACCESSION   DACEEZ010000177 DACEEZ010000000
VERSION     DACEEZ010000177.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639966
            Sequence Read Archive: SRR10023158
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1753)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1753)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741683.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741683.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:44:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,016
            CDSs (total)                      :: 2,962
            Genes (coding)                    :: 2,889
            CDSs (with protein)               :: 2,889
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 36 of 73
            Pseudo Genes (incomplete)         :: 29 of 73
            Pseudo Genes (internal stop)      :: 18 of 73
            Pseudo Genes (multiple problems)  :: 8 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1753
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639966-rid9879223.denovo.187"
                     /isolate="E20313_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            949..1422
                     /locus_tag="IWO89_002873"
     CDS             949..1422
                     /locus_tag="IWO89_002873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2975 domain-containing protein"
                     /protein_id="HAQ0457561.1"
                     /translation="MKFKTLLLKFVVLGISAVFILFGVLFYTQIATSEKGFNFDWQTT
                     LFVSVIYFALLLGIVIAYFLLKLLKKIEDNQAFSTQSLNILKKIERTILFIGFAFFGL
                     LPKFMKVADLDDAPGLMLVGIAVVFIPFAVYTLTSVLEKLLEHAILLKTDHDLTV"
     gene            1431..1658
                     /locus_tag="IWO89_002874"
     CDS             1431..1658
                     /locus_tag="IWO89_002874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008266675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAQ0457562.1"
                     /translation="MAIIVNLDVVLAQKKMSVTELSEKVGITMANISILKNGKAKAIR
                     FSTLEKICQVLECQPGDLLTYKNDSNTIPEE"
BASE COUNT          588 a          272 c          260 g          633 t
ORIGIN      
        1 ttttcggaga attaaaaaat acaataaata actttagtta ctttaatgcg ttcttacttt
       61 atttttatat tatcattttt cttattcact tctttggttt atactattct tataaataaa
      121 aacgaggtat gtcctatgat gaaaagattg tactattcat tgatcataac gattggctat
      181 ttgattgtat caaatctagg aaatatggta tttggtatca gtaaagaatt cagttggact
      241 actacattat gggaatctct attctttttc atcttcgtat ttttgttaca aaattatcga
      301 aaaaaataaa catgtgccat ctatctgttg gcacatgttt taggatttat ggtacttttt
      361 attcggcaat ccattttatt tttctacttc ttcttaatac ctctaacact tttgatttat
      421 aaactaatcc aatgcatcta cttgcgctta ttaagctatt tcagctagat agtaccaccc
      481 catgcgtcaa tgcttgattt tccttttgta taacattgta taagcaataa aggacaaata
      541 agtggtgatt acaccaaacc attgaataag aaaaattctg atacgtaaat aaacatgaac
      601 ttgaaaaaat taatgaatcg gttagctact acgtagttgt cttttttctc atacagttct
      661 gttaacttac acttctcttc gctagataat aaaaaatcct ccaactgtat tgtccaatat
      721 aatcccaggc atctaaacta gcattataga agagacacac atagaatcta aaataactgg
      781 ttttaaatgt ttcttacaat caaaaaaagc tgttaaatgc actccacacc ttcatactct
      841 atcatttttt ataaacgata tatttttatt gtttttcgat aaaaaatagt tgttttacaa
      901 taattattct gctatatttt agctatcaaa gataaaagag ggataaaaat gaaatttaaa
      961 acattgctat taaaatttgt cgttttaggt ataagtgccg tatttatttt gttcggcgta
     1021 cttttttata ctcagatcgc tacaagcgaa aaaggattca attttgattg gcagaccaca
     1081 ttatttgttt ctgtcattta ctttgccctt ctcctaggga tcgttattgc ttatttctta
     1141 ttgaaactat tgaaaaaaat cgaagataac caagcttttt ccactcaaag cttaaacata
     1201 ctaaaaaaaa tcgaacgaac cattttgttt attggtttcg ccttttttgg cttacttcct
     1261 aaatttatga aagtagcgga tttagacgat gctccaggtt tgatgttggt aggaatcgct
     1321 gtcgtattca ttccttttgc agtatacaca ttaacaagcg tgctggaaaa actgcttgaa
     1381 catgcgattt tacttaagac agatcatgat ttgacagtgt gaggtgcgac atggcaatta
     1441 tcgttaattt agatgtagta ttagctcaaa aaaaaatgag tgtgactgaa ttgagtgaga
     1501 aagtgggcat tacgatggca aacatctcta ttctaaaaaa tggaaaagca aaagccatcc
     1561 gtttttccac tctcgaaaaa atctgccaag ttttggagtg tcaaccaggt gatttgttga
     1621 cctataaaaa tgactcgaat actataccag aagaataaaa taaagtagaa caaacagtaa
     1681 ataaaaaata atctgaatga tagatgtgtg agacttttag aagttattta cgtctattat
     1741 tcagattttt tat
//
LOCUS       DACEEZ010000178         1664 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20313_2017
            SAMN12639966-rid9879223.denovo.188, whole genome shotgun sequence.
ACCESSION   DACEEZ010000178 DACEEZ010000000
VERSION     DACEEZ010000178.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639966
            Sequence Read Archive: SRR10023158
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1664)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1664)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741683.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741683.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:44:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,016
            CDSs (total)                      :: 2,962
            Genes (coding)                    :: 2,889
            CDSs (with protein)               :: 2,889
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 36 of 73
            Pseudo Genes (incomplete)         :: 29 of 73
            Pseudo Genes (internal stop)      :: 18 of 73
            Pseudo Genes (multiple problems)  :: 8 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1664
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639966-rid9879223.denovo.188"
                     /isolate="E20313_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            131..505
                     /locus_tag="IWO89_002875"
     CDS             131..505
                     /locus_tag="IWO89_002875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster biosynthesis family protein"
                     /protein_id="HAQ0457563.1"
                     /translation="MYLKITEQAQERLQKYIDEGATVILDLDDGVGEYSKMGVCSLDT
                     SFRLLLLDKEQRKNDYKLQLDSDIGDVYIKDYSKMYMDEHMTLSLDPRLGVFTLESPS
                     GSLDSHVQLVDLRPEKFSGIKQ"
     gene            complement(619..1248)
                     /locus_tag="IWO89_002876"
     CDS             complement(619..1248)
                     /locus_tag="IWO89_002876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HAQ0457564.1"
                     /translation="MKIVFLHGLGQDEQSWKDVISYLPNSYSCKSLSLFNHLNSTDTA
                     TLDDLTKYIEVQLNQIKEPFILCGLSLGAVIALNYLATPSPYLKKVVLVAPQFESPNR
                     LLLTFQNIYFYCVPSKIFEKLGLSKKQTLTLLRSLETLDCKKQLRTSRLPKLILCGTK
                     DHANKPAAKRLYKLSTNGRLLFIKNGKHELNREKPEIMAQAMRSFIENS"
     gene            complement(1382..1454)
                     /locus_tag="IWO89_002877"
     tRNA            complement(1382..1454)
                     /locus_tag="IWO89_002877"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1419..1421),aa:Thr,seq:ggt)
BASE COUNT          584 a          240 c          283 g          557 t
ORIGIN      
        1 ctacactctg agatgtaaga aatatcataa gatatttctt acatcttttt gttttgagca
       61 ggactttaga taattttatt aatatatgct agcatgcttt tgtaagaaaa agaaagaggg
      121 cgtattagtt atgtatctta aaattacgga acaagcacaa gaaagattac aaaaatatat
      181 tgatgaaggc gcaacagtta tattggattt agatgatggc gttggggaat attctaaaat
      241 gggtgtttgc tctttagata ctagttttcg tttacttctt cttgataaag aacagagaaa
      301 aaatgattat aaactccaat tagatagtga tattggggat gtttatataa aagattattc
      361 aaaaatgtac atggatgaac atatgaccct ttctttagat ccacgtctag gtgtatttac
      421 tcttgaaagc ccaagtggtt ctttagacag tcatgttcaa ttagtagatc tacgaccaga
      481 aaaattctct ggaataaaac aataactttc tctctattgt ccataaaact agtctcaatc
      541 aagctgtatt gaaaaaacaa gacaacattc ttaccatggt aaataaaaag acgacacatt
      601 ttgtcgtctt tttttaaatt aactattttc gataaaagat cgcatagctt gtgccattat
      661 ttctggcttt tctctattca attcatgttt tccgttttta ataaagagta gccggccatt
      721 cgttgaaagt ttatataaac gttttgctgc tggtttattc gcatgatctt tggtaccgca
      781 taaaatcaat ttaggaagtc gtgatgtacg aagctgtttt ttgcagtcta atgtttcaag
      841 tgaacgaagt agggtaaggg tttgtttttt agatagacct aatttttcaa atatcttaga
      901 aggtacgcag taaaaataaa tattctggaa agtaagcaat aagcgattag gagactcaaa
      961 ttgtggagcg actaaaacaa ccttttttaa atatggagaa ggagttgcaa gatagtttaa
     1021 agcaataaca gcgcctaaag ataatccaca taaaataaaa ggttccttta tctgattcaa
     1081 ttgtacttcg atgtatttgg ttaagtcgtc taaagtagca gtgtctgtgc tgtttaaatg
     1141 attgaacaat gaaagacttt tgcaagaata actattgggt agataagaga ttacatcttt
     1201 ccatgattgt tcgtcttggc ctagaccatg caaaaaaaca attttcatat tgctctcctt
     1261 tttatagtta tcatatagac tttctttgta ctattaaaat ataaacattc atttcttacg
     1321 tcataaaata aaaaaacagc gcagacacat tgttaaacca acatgttgca ctgtttaagg
     1381 aagccacttg ccgggcttga accggcgacc tcttccttac catggaagcg ctctaccagc
     1441 tgagctaaag cggcattatc attaatacaa ataataggat aacgaatatt aatgtatttg
     1501 tcaacagaat tttttcgaaa aaatgtgttt ctctaatagg aaaacagatt ttagaaaaaa
     1561 taaaaataac aaaaaatgcg aacggtttct ttgataatat aataaaaaaa ttcaaaaaaa
     1621 gtattgcgtt atttagaaga aattgatatt atattcatgt actt
//
LOCUS       DACEEZ010000179         1553 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20313_2017
            SAMN12639966-rid9879223.denovo.189, whole genome shotgun sequence.
ACCESSION   DACEEZ010000179 DACEEZ010000000
VERSION     DACEEZ010000179.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639966
            Sequence Read Archive: SRR10023158
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1553)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1553)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741683.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741683.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:44:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,016
            CDSs (total)                      :: 2,962
            Genes (coding)                    :: 2,889
            CDSs (with protein)               :: 2,889
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 36 of 73
            Pseudo Genes (incomplete)         :: 29 of 73
            Pseudo Genes (internal stop)      :: 18 of 73
            Pseudo Genes (multiple problems)  :: 8 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1553
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639966-rid9879223.denovo.189"
                     /isolate="E20313_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            191..445
                     /locus_tag="IWO89_002878"
     CDS             191..445
                     /locus_tag="IWO89_002878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HAQ0457565.1"
                     /translation="MGKELKVRKIGNSVGVILPSSLGLKSGDIIQAKQEDNLFILDTT
                     QIAKEHDRKLIEESFQDFEKGLTVSEIEMVKAFGKYGWSE"
     gene            446..763
                     /locus_tag="IWO89_002879"
     CDS             446..763
                     /locus_tag="IWO89_002879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HAQ0457566.1"
                     /translation="MKEYTLVYSKSFQTSLETIIEEWENELALSPASIKRFVGAIQKA
                     LELTKNFPTMHEEVSEIYGMDIPTYRIVIGKSYALFYRIDHEKKEILVGRIFQSKQMK
                     LGF"
     gene            complement(1397..>1553)
                     /locus_tag="IWO89_002880"
     CDS             complement(1397..>1553)
                     /locus_tag="IWO89_002880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS982 family transposase"
                     /protein_id="HAQ0457567.1"
                     /translation="SSLEKLGCQNFNSRSVKGLESRFESILLAYSVLLSRAQRRFEGT
                     LRYSLGY"
BASE COUNT          552 a          231 c          293 g          477 t
ORIGIN      
        1 gatgttaacg gaagtgtatg gtgagtagtt cactcgtatt ttgatatcgt ttgtatatgt
       61 tttcagcgtt ctaagtggct tattttgtcc aataataata tggagtggaa tcccttaaga
      121 aacaccgttg gcaattgttg ttataacatg ttataattta ttcatcaaaa actaaaagga
      181 gtgtgataac atgggaaaag aactaaaggt tagaaagatc ggcaatagcg tgggagtgat
      241 ccttccaagc tctcttggat taaaaagcgg agacattatc caagccaaac aagaagacaa
      301 cttattcata ttagatacta ctcaaatagc aaaagaacac gatcgcaaat tgattgaaga
      361 aagtttccaa gactttgaga aaggtttaac agtttcagaa attgaaatgg taaaagcttt
      421 cggaaaatac ggctggagtg aatagatgaa agagtacacg ctagtttatt ctaaaagttt
      481 tcagaccagc cttgaaacga taatagaaga atgggaaaat gagcttgctc tttctccagc
      541 cagcataaaa agatttgtag gtgcgatcca gaaggcttta gaactaacca aaaattttcc
      601 aactatgcat gaggaagttt ccgaaatcta tggaatggat atcccaacat atcggatcgt
      661 gatcggcaaa agctatgcct tattttatcg gatcgaccat gaaaaaaaag agatacttgt
      721 aggacgtatt ttccaatcta aacaaatgaa actaggcttt taacaataat taggaaaaag
      781 tcgctcatcc tctacaggtg ggtgattaat gccgctcgat atggttgtta accgtcggtt
      841 actcgaagat tgatatataa gacttagtac ctttttattt tgctccatgt tatggccgaa
      901 ggtctaaaga tacaatcatt agtctagaga aaaattaaga accttaggaa ctacggggat
      961 cgcttggtga ataagagaca ctgctattag tgaaaaaata agatatcaag tacgctctat
     1021 tcccaaaaat ttcgtgactt tactcatgag aggttcaagt agtatatttt taatcaaaat
     1081 tgacgtaact tttaataaat actacctaaa taaggtagta tttatttttg tgttttaaaa
     1141 gaggaagtcc aatatatcta tgggaaactt cttaaatatt atgttggtac atgaaaatat
     1201 acttccctgc tagactattt acaacatttt actttgtacg tattcttgtt ttaaaaggaa
     1261 tgggataaaa tataggtaca ataactattt gatgctaaat ataattaatg gaggagagat
     1321 agtgaacata cttaatgaaa aaagatatgt atggtttgct ggtaaaaaat atattaaccc
     1381 gtggtgctag ttaattttaa tatcctaaag aatatctcaa agttccttca aaacgtcgtt
     1441 gtgctcgact taatagaaca ctgtaagcca gtaatatgct ttcaaatcta ctctctagcc
     1501 ctttaacaga acgtgagttg aaattttgac aacctaattt ttctaaggaa gaa
//
LOCUS       DACEEZ010000180         1528 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20313_2017
            SAMN12639966-rid9879223.denovo.190, whole genome shotgun sequence.
ACCESSION   DACEEZ010000180 DACEEZ010000000
VERSION     DACEEZ010000180.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639966
            Sequence Read Archive: SRR10023158
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1528)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1528)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741683.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741683.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:44:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,016
            CDSs (total)                      :: 2,962
            Genes (coding)                    :: 2,889
            CDSs (with protein)               :: 2,889
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 36 of 73
            Pseudo Genes (incomplete)         :: 29 of 73
            Pseudo Genes (internal stop)      :: 18 of 73
            Pseudo Genes (multiple problems)  :: 8 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1528
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639966-rid9879223.denovo.190"
                     /isolate="E20313_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            88..939
                     /locus_tag="IWO89_002881"
     CDS             88..939
                     /locus_tag="IWO89_002881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0457568.1"
                     /translation="MKSDDWIEHTWLPLKRNSKRKKFWVLQLWPLGKLCSYGARLILD
                     LTVYSIGVFGTAYYLINDPNRIAASDIFTVLMTLIFVMLLSIFTQLVMQFTRQKLTAG
                     KLFKSLFDLIVVNAILATLIKQIEQSGRTTKGVKDFLLTFIHFVNGHMYLFEVGIILF
                     FVGYVSTKLIKRSIEKEIEIDLIPTLLGYEYNVDFGRYDELINTDIVHTSIHESTVKG
                     IRSILKNGQEMKAIDRRKEIYNFYEVEASLRYGIGKLWLDGSNIRQNKPYIFWNSSDL
                     ELPMKDK"
     gene            960..1172
                     /locus_tag="IWO89_002882"
     CDS             960..1172
                     /locus_tag="IWO89_002882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0457569.1"
                     /translation="MIPLYRIVLIVGMELKTLKKEQLFKVKERHNQLLCSKTGTMNIE
                     YRTEYLELTNQTDFLLIRKQQEQEKN"
BASE COUNT          568 a          201 c          310 g          449 t
ORIGIN      
        1 cttaaaaaat tggttcattg agcaacctat cgctgctaaa gtagcaaagg aaaacgttaa
       61 aggaaagaat ttagggaaga aatcgaaatg aaatcggacg actggattga acacacctgg
      121 cttcctctga aaagaaatag caaacgaaag aaattttggg tattgcaatt atggccatta
      181 ggaaaacttt gtagttacgg ggcacgactt attcttgatt taaccgttta ctcgattgga
      241 gtatttggaa cggcttatta cttgataaat gatcccaata ggattgcagc atcagacatt
      301 tttacagtgc taatgacgtt gatttttgtc atgctattgt ctatttttac tcaattagtc
      361 atgcaattta ccaggcaaaa attaacagca ggaaaacttt tcaaaagcct attcgattta
      421 attgttgtaa atgcaattct agcgacttta ataaaacaaa ttgaacaatc tggaagaaca
      481 acaaaaggag tgaaggattt tttattgacg tttattcact ttgtaaatgg acacatgtat
      541 ttgtttgaag tgggtattat tctctttttt gtcgggtatg tatccacgaa acttattaaa
      601 cgttcaattg aaaaagaaat tgaaattgac ctgattccga cacttcttgg ttatgagtac
      661 aatgttgatt ttggaagata tgacgaattg atcaatacgg atatcgttca cacgagtatc
      721 catgaatcta ctgttaaggg catcagatct attttaaaaa atggccagga aatgaaagca
      781 atagatcgaa gaaaagagat ctacaatttt tatgaagtcg aagcaagcct acgatatggc
      841 atcggaaaat tgtggttaga tggaagtaat attcgtcaaa acaaaccata cattttttgg
      901 aacagtagtg atttagaact tccaatgaaa gataaataat tatggagaat cgtttgaaaa
      961 tgattcctct atacaggatt gtgcttatag ttggcatgga gttaaaaacc ttaaagaagg
     1021 agcagttatt caaagtgaaa gaacgtcata atcagttact atgtagtaaa acgggtacga
     1081 tgaacattga ataccgaaca gaatatttag aattaacgaa tcaaactgat tttttgttga
     1141 ttcgaaagca acaagagcaa gaaaaaaatt aatggctact tatagccaat tgattgatga
     1201 atgggaacaa ttcaaggaat ctaaaattcc agaacgtctc taaaagcctt tatttgtaaa
     1261 gagcataagg aaacgtggga acacttttta aaaaaagtgt tccaataggc tgtgcctatt
     1321 acgtttctca aaaataaatg tgggaacaaa atatttaaaa ataggatagt gaaaggatag
     1381 tgaaggatag taaaaggagg gtagtaggat agcaaaaagg ataggctcct tttcgctatc
     1441 ctttttaaat tggaggaatt aatatggaag taagaataag aaatgtggat gagaaaatcg
     1501 taagagaaat tgatcgaaga tgtgacga
//
LOCUS       DACEEZ010000181         1494 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20313_2017
            SAMN12639966-rid9879223.denovo.191, whole genome shotgun sequence.
ACCESSION   DACEEZ010000181 DACEEZ010000000
VERSION     DACEEZ010000181.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639966
            Sequence Read Archive: SRR10023158
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1494)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1494)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741683.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741683.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:44:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,016
            CDSs (total)                      :: 2,962
            Genes (coding)                    :: 2,889
            CDSs (with protein)               :: 2,889
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 36 of 73
            Pseudo Genes (incomplete)         :: 29 of 73
            Pseudo Genes (internal stop)      :: 18 of 73
            Pseudo Genes (multiple problems)  :: 8 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1494
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639966-rid9879223.denovo.191"
                     /isolate="E20313_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            139..477
                     /locus_tag="IWO89_002883"
     CDS             139..477
                     /locus_tag="IWO89_002883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0457570.1"
                     /translation="MPDYKQKAGYSFCSDKFQLGKFTKLAGEEVSTVRIKECPLQIET
                     LVQRISTRQDFAIVEREIVAIFVEEGILHDPTHINVDKWQPLIYKFREYTTANQSLTL
                     NFRFQEFKES"
     gene            complement(682..1257)
                     /locus_tag="IWO89_002884"
     CDS             complement(682..1257)
                     /locus_tag="IWO89_002884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF159 family protein"
                     /protein_id="HAQ0457571.1"
                     /translation="MCGRYFFDLESELLQNYYREAQTKQPKQAAELAAGEVFPSNLVV
                     TLRKEEENILTPEIMKWGFTGFKKGQLMINARAETVEEKKTFHQPFLQNRCVFPMSGF
                     YEWNEEKDKFMFSNKEKIVYIGGFYRSHQDEKMAESIIMTTEPNRTVAPIHDRMPLVL
                     TEEQIEPWVTDISFARKIITQQMPELVMEKV"
BASE COUNT          462 a          311 c          253 g          468 t
ORIGIN      
        1 actaaaaaaa taatccaaga aacattgaac ctatggatcc aaaagctacc caaatactat
       61 agctaacccc ccgctacctt caattttgca gataaaacgc tttgggagaa aatttcaaag
      121 acaacaggcc atcctatcat gcccgattat aaacaaaaag ctggctattc cttttgctct
      181 gacaaatttc aattgggaaa gttcacgaaa ctagcaggtg aagaagtttc aactgttcgg
      241 atcaaagaat gcccgcttca aatagaaaca ttggttcaac gaatttcaac tagacaagac
      301 tttgctattg tagaacgtga gatcgtagct atttttgtag aagaagggat tttacatgat
      361 ccaactcata tcaatgtgga taaatggcaa cctcttatct ataaattcag agaatataca
      421 actgccaacc agtcgttaac tttgaatttt agattccaag aattcaaaga gtcataatga
      481 taaacatcgt taggtataaa taaaaaagac tcgcagcacg agtaaaaaac aaatagaagc
      541 tatacattag aaaggtcgac tgtgacccta tagatgcagt atatgcagct tatgagcatt
      601 tcttctgttc tctttgtttt tgggatctgt gctgaagtct ttttcgtttt ttgccgctct
      661 ttctttgaaa aatcgattat gttatacctt ctccattact aattcaggca tctgctgagt
      721 aatgattttt cgcgcaaaag aaatatctgt tacccaaggt tcgatttgtt cttcagtcaa
      781 gacaagcggc attcggtcat ggattggtgc gactgtgcgg ttaggttcag tagtcattat
      841 gattgattct gccatttttt catcctgatg gctacgataa aaaccgccaa tatacactat
      901 tttttcctta tttgaaaaca taaatttgtc tttttcttca ttccactcat aaaagccact
      961 cattgggaaa acacaacgat tttgcaaaaa tggttgatga aaggtctttt tttcttctac
     1021 tgtctctgca cgggcattaa tcatcaattg tcctttttta aatccggtaa atccccactt
     1081 catgatttcc ggagtgagga tgttttcttc ctcttttctt aacgtcacaa caagatttga
     1141 cggaaaaact tctccagctg ctaattctgc tgcctgcttc ggttgtttcg tctgggcttc
     1201 tcgataataa ttctgaagga gctctgactc tagatcaaaa aaataacgac cacacatcct
     1261 aatccctccc aatctttttt tctatcatac actgcaacaa gaagttcgca ccaacaaaac
     1321 gctttcaatt tttgtagaac taaaaaagtt gtgacaagtg acaaactctc cagtgaatgg
     1381 tattctcctg ccaactcctc ggtcacaagt cacaatattc aacaaacatg agaagctgct
     1441 ttttgaaatt gttccttgtg actcggagtg aaacggcagg cttacgacct ctgg
//
LOCUS       DACEEZ010000182         1471 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20313_2017
            SAMN12639966-rid9879223.denovo.192, whole genome shotgun sequence.
ACCESSION   DACEEZ010000182 DACEEZ010000000
VERSION     DACEEZ010000182.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639966
            Sequence Read Archive: SRR10023158
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1471)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1471)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741683.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741683.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:44:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,016
            CDSs (total)                      :: 2,962
            Genes (coding)                    :: 2,889
            CDSs (with protein)               :: 2,889
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 36 of 73
            Pseudo Genes (incomplete)         :: 29 of 73
            Pseudo Genes (internal stop)      :: 18 of 73
            Pseudo Genes (multiple problems)  :: 8 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1471
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639966-rid9879223.denovo.192"
                     /isolate="E20313_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(59..421)
                     /locus_tag="IWO89_002885"
     CDS             complement(59..421)
                     /locus_tag="IWO89_002885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1827 family protein"
                     /protein_id="HAQ0457572.1"
                     /translation="MKLIETPVNSNLNIKTFYPKVVEFFFGNTAINYYKLFSLDRTQL
                     LLVDTYDKKQVVMINTKKKITRQEIDYAIHHVLKMTREDVKVHVGVKQELERAGIQFK
                     RPNKDIVVIEQKNTKDSI"
     gene            644..1396
                     /locus_tag="IWO89_002886"
     CDS             644..1396
                     /locus_tag="IWO89_002886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HAQ0457573.1"
                     /translation="MEYTIKKMSEISGVSPRTLRFYDEIGLLKPARINSSGYRIYGKK
                     EVDRLQHILFYRTMAFKLDDIQEVLDNPSFDHQKALIKHQQMLLEKRAQIDTLLTTVQ
                     QTLDMYEGGRKMSDKEKFEGFKQQKLKENEESYGQEIRQKYGKKKVTASNEKWMNMDK
                     ETYEEMQTVEKQMLFDLTLYLKEPEDESLADRIFQAHKKWLSYSWPEYQPKAHKGLGM
                     MYEADERFTAYYDERSGHGAAKALNEIIQKKA"
BASE COUNT          521 a          249 c          257 g          444 t
ORIGIN      
        1 ttttattgta tacattagat atggagcagg ttaccataaa gatgattatt agtcgttatt
       61 aaatagaatc cttcgtattt ttttgttcta tcactacaat atctttattt ggacgtttga
      121 attgtattcc ggcacgttct agctcctgtt tcactcctac atggactttg acatcttctc
      181 gtgtcatttt taaaacgtga tgaatcgcat agtcgatttc ttgccttgtg atttttttct
      241 tcgtattgat catcacgact tgctttttat catatgtgtc taccaatagc agttgtgttc
      301 gatctaacga aaataatttg taataattaa tagccgtatt cccaaaaaag aattccacta
      361 ctttaggata aaatgtctta atatttaaat tactattcac aggtgtttcg attagcttca
      421 tctcattcct cttttctata tagtcgattt agatccaatc ttaagttatg ttaccatctt
      481 tcctagcatt aaggaatcgg ttacaattga acctttaaag acaaaaggat ttacctttta
      541 tcacttaata gtataacata atttcatgag aaaaaaatac aattttttct tgaccatctc
      601 gttacgtaaa ggattatgct tacatttgtc aggaggaaaa ataatggagt acacaataaa
      661 gaaaatgtca gagatatcag gagtcagtcc gagaaccttg cgcttttatg atgagattgg
      721 actgctaaaa ccagctagga tcaattcttc aggttatcgg atttatggca aaaaagaagt
      781 cgatcgtctg caacacatct tattttatcg aacaatggca ttcaagttgg atgatatcca
      841 agaggtctta gacaatccat cttttgacca ccagaaagct ttaatcaagc atcagcaaat
      901 gttgctggaa aaacgggcac aaatcgatac cctcttgaca actgtccaac agacacttga
      961 tatgtacgaa ggaggaagaa aaatgagtga caaagaaaaa tttgaagggt ttaaacaaca
     1021 aaagttgaaa gaaaatgaag aatcctatgg acaagagatt cgacaaaaat acggaaaaaa
     1081 aaaggttaca gcatccaatg aaaaatggat gaatatggat aaagaaacgt atgaagaaat
     1141 gcaaactgtt gaaaagcaga tgttatttga tctcactctt tacttaaaag aaccagaaga
     1201 tgaaagctta gcagaccgta tcttccaagc tcataagaag tggttatctt acagctggcc
     1261 tgaatatcag ccaaaagcgc ataaaggact aggaatgatg tacgaagccg atgaacggtt
     1321 cactgcatat tatgacgagc gaagtggtca tggtgcagca aaagctttaa atgaaattat
     1381 ccaaaaaaag gcgtaatctt ttacatgatc caacaaaaaa gtacacgtaa aatgacctaa
     1441 taacaaaaca gcagacaccc aagttagatt t
//
LOCUS       DACEEZ010000183         1464 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20313_2017
            SAMN12639966-rid9879223.denovo.193, whole genome shotgun sequence.
ACCESSION   DACEEZ010000183 DACEEZ010000000
VERSION     DACEEZ010000183.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639966
            Sequence Read Archive: SRR10023158
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1464)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1464)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741683.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741683.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:44:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,016
            CDSs (total)                      :: 2,962
            Genes (coding)                    :: 2,889
            CDSs (with protein)               :: 2,889
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 36 of 73
            Pseudo Genes (incomplete)         :: 29 of 73
            Pseudo Genes (internal stop)      :: 18 of 73
            Pseudo Genes (multiple problems)  :: 8 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1464
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639966-rid9879223.denovo.193"
                     /isolate="E20313_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            <1..820
                     /gene="dnaI"
                     /locus_tag="IWO89_002887"
     CDS             <1..820
                     /gene="dnaI"
                     /locus_tag="IWO89_002887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="primosomal protein DnaI"
                     /protein_id="HAQ0457574.1"
                     /translation="FLSAHQEELTQADIEKSYAKLYEFVQEKRKYQKNDPTMIAPGYE
                     PKLTLNFHYVDVTYVPTEALLARQRQEEIRSRVKAMDMPKDIQSASFENFERTDGRGY
                     ASLEALDFVEKYTADPKGYHKGLYLVGSFGIGKTYLLGATARELAIAGFTTTLVHFPT
                     FAVEMKQAIGKDQVAEKLDAVKRSPILMIDDIGADAMSSWIRDEVFGVILQYRMQEQL
                     PTFFSSNFTMDELEQHLSVTQRGDEEPLKAKRIMERIRYLTKEIEMTGRNRRNG"
     gene            949..1389
                     /locus_tag="IWO89_002888"
     CDS             949..1389
                     /locus_tag="IWO89_002888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HAQ0457575.1"
                     /translation="MRAIFSSISVVGLFFLLAGCGSADSNTGSVDSIDAGQTVKTSST
                     KTERQSIFVGTIESITQVEAEEETLQLKLVDVMAKEDPANMGTSFSNDGVILNATAEK
                     ISVDINELKIGDKVQFTLVEMPIMTMSIPPQVPGDSIIDVSLNK"
BASE COUNT          510 a          244 c          334 g          376 t
ORIGIN      
        1 ttttctaagc gcacaccaag aagaattgac acaagcggat attgaaaaaa gttacgctaa
       61 attgtatgaa ttcgtgcaag aaaagcgtaa atatcaaaaa aacgatccaa cgatgatcgc
      121 tcccggctat gagccgaagc tgacgttgaa ttttcattat gtggatgtca cttatgtacc
      181 aacggaagca ttgttagcac ggcaaagaca agaagaaatc agaagccgag taaaagcaat
      241 ggatatgcca aaagatattc aatcggcttc ttttgaaaac tttgaacgta cagatggacg
      301 aggatatgcg agcttagaag ccctagattt tgtagagaaa tatactgctg atccaaaagg
      361 gtatcataaa ggactgtatc tagtaggaag ttttggtatt ggaaaaacat atttattagg
      421 agcaacagcg agagaattag cgattgctgg attcaccacg acgctagtcc attttccgac
      481 atttgccgtt gagatgaaac aggcaatcgg aaaagatcaa gtggcagaaa agttagatgc
      541 agtgaaacgt tcacctattt tgatgatcga tgacattggt gcagatgcga tgagtagttg
      601 gatacgagac gaagtattcg gtgttatcct tcaatatcgg atgcaggaac agttaccaac
      661 cttcttctca tcgaatttta caatggatga attggaacag catttgtccg tcactcaacg
      721 tggagatgaa gagccgctaa aagcgaaacg gataatggaa cgcatacgtt atttgacaaa
      781 agaaatcgaa atgactggtc gcaaccgacg caatggttga acagttgaca agtccaactg
      841 acatttgacc tagtagtaaa agcaggtcag cgaatcatta tatggacaga aatgaccgga
      901 gacaatcacc gcaacaagta atgcagaaac ttaaacggag gattttttat gagggctatt
      961 ttttctagta tatctgtagt tggtctattt tttcttttag caggctgcgg tagcgcagat
     1021 agcaacacag gttctgttga ttcgattgat gcgggtcaaa cagtaaaaac aagcagtacg
     1081 aaaacggaga gacaaagcat atttgtcgga acgattgaaa gtattactca agtagaagca
     1141 gaagaagaaa cgcttcagtt gaaactcgtc gatgtcatgg ccaaagaaga tccagcgaat
     1201 atgggaacgt cattcagcaa tgacggagtg atcttaaatg ctactgctga aaaaataagc
     1261 gtggacataa atgaattgaa aatcggtgac aaagtccaat ttacattagt tgagatgccg
     1321 atcatgacga tgtctatccc accgcaagtt cctggagatt cgattattga tgtttcattg
     1381 aacaaataac caataacatt ttttcaaaaa taagaaaatg ttgttttata gaacgaaaag
     1441 gtatattttt taaggagata tggg
//
LOCUS       DACEEZ010000184         1451 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20313_2017
            SAMN12639966-rid9879223.denovo.194, whole genome shotgun sequence.
ACCESSION   DACEEZ010000184 DACEEZ010000000
VERSION     DACEEZ010000184.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639966
            Sequence Read Archive: SRR10023158
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1451)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1451)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741683.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741683.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:44:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,016
            CDSs (total)                      :: 2,962
            Genes (coding)                    :: 2,889
            CDSs (with protein)               :: 2,889
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 36 of 73
            Pseudo Genes (incomplete)         :: 29 of 73
            Pseudo Genes (internal stop)      :: 18 of 73
            Pseudo Genes (multiple problems)  :: 8 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1451
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639966-rid9879223.denovo.194"
                     /isolate="E20313_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(<1..625)
                     /locus_tag="IWO89_002889"
     CDS             complement(<1..625)
                     /locus_tag="IWO89_002889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4062 domain-containing protein"
                     /protein_id="HAQ0457576.1"
                     /translation="MKTINLIKVFIASPGDIAAKRNEVEETIYRWNIENTDNTKVILM
                     PIRWETNAIPTYINNSSGQSVINKQIVENSDILLAIFGHKLGSPVDNYRSGTIAEIDY
                     FYKNSKGEVGVFFLDEPVPQVLAEEYIEVKKYRDSLEDKGFYGTYSKDNIRRFLTKKV
                     NYLIEKAQNNLESKRFDLDSISYKTPSVINKKVTGTDKDFDDNLDIFD"
     gene            complement(784..1050)
                     /locus_tag="IWO89_002890"
     CDS             complement(784..1050)
                     /locus_tag="IWO89_002890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAQ0457577.1"
                     /translation="MMIKKLIEEKQKDKDFAEAYDREGRKLETAVALYHAREEAGLTQ
                     AELAQKAHTTQSTIARIERGDNVSFDKLDDIARALGKKLVVEFA"
     gene            complement(1040..1396)
                     /locus_tag="IWO89_002891"
     CDS             complement(1040..1396)
                     /locus_tag="IWO89_002891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HAQ0457578.1"
                     /translation="MEKISFETFKRPNGHDEFLEWLETLPKKDSAKLLRTIEETEKNG
                     MLVAQRLKWVKKLDTNLYELRSKVGSNIQRAIYFHVVNGRYVITHGFTKKTEKTPISE
                     LKHAKELREEWFTNDD"
BASE COUNT          405 a          279 c          181 g          586 t
ORIGIN      
        1 gatcaaaaat atctaagttg tcatcaaaat ctttatcagt tccggtaact tttttgttaa
       61 ttactgaagg tgtcttgtaa gaaatagaat ctaaatcaaa gcgttttgat tctaaattat
      121 tttgtgcttt ctcaattaaa taattcactt tttttgtgag aaaccttctg atattgtctt
      181 tgctatatgt tccataaaaa cccttatctt ctaaagagtc cctgtacttc ttaacttcta
      241 tatactcttc agccaaaact tgtggaactg gttcatctaa gaaaaaaacc cctacctcgc
      301 ctttgctatt tttataaaag taatcaattt ctgctatagt tcccgatcta taattatcaa
      361 ctggagaacc caacttatgg ccaaaaatcg ctaataatat atcactattt tctacaattt
      421 gtttattaat aacactttgc ccagagctat tatttatata agtaggaatc gcattcgttt
      481 cccatcgaat tggcatcaaa attacttttg tattatctgt attttctata ttccatctat
      541 atatggtttc ttctacttca ttcctctttg ctgctatatc acctggtgat gctataaata
      601 cttttattag atttatggtt ttcatttatt gtacctttct ctttatgtct ctttaaagta
      661 atttttgtat aaaatttatt gacttttaca ctataggaca acttttatct atttagtaca
      721 aaaaggccca ctaaactaat tgattagtta ggagcctatt acaggtatat tttcaactcg
      781 cttttaagca aattctacta ccagcttttt gcctaatgcc ctagcgatat catcaagttt
      841 atcgaaagat acgttatccc ctctctcaat tcgagcaata gtagattgag ttgtatgtgc
      901 tttttgtgca agctccgctt gagttaggcc tgcttcctcc cgtgcatgat aaagagcaac
      961 cgcagtttcg agttttcttc cttcacggtc atatgcttct gcaaaatctt tgtctttttg
     1021 cttttcttca atcaattttt taatcatcat ttgtgaacca ctcctctcta agttcttttg
     1081 catgtttaag ttcacttatt ggtgttttct ccgttttttt agtaaatcca tgggtaatga
     1141 cataccttcc attgaccaca tggaaataga tagctctttg gatgtttgat cctaccttac
     1201 ttcttaattc gtacaaatta gtgtctaatt ttttcaccca tttcaaccgt tgagctacta
     1261 gcatcccatt cttttctgtt tcttctattg tacgtaaaag cttcgcacta tctttttttg
     1321 gtaatgtttc caaccattct aaaaattcat catgaccatt aggtctttta aatgtttcaa
     1381 aagatatttt ttccataccc aattatatca tatattgatt ttatattata gtatataaca
     1441 taaaaattcg a
//
LOCUS       DACEEZ010000185         1418 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20313_2017
            SAMN12639966-rid9879223.denovo.195, whole genome shotgun sequence.
ACCESSION   DACEEZ010000185 DACEEZ010000000
VERSION     DACEEZ010000185.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639966
            Sequence Read Archive: SRR10023158
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1418)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1418)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741683.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741683.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:44:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,016
            CDSs (total)                      :: 2,962
            Genes (coding)                    :: 2,889
            CDSs (with protein)               :: 2,889
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 36 of 73
            Pseudo Genes (incomplete)         :: 29 of 73
            Pseudo Genes (internal stop)      :: 18 of 73
            Pseudo Genes (multiple problems)  :: 8 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1418
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639966-rid9879223.denovo.195"
                     /isolate="E20313_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            149..232
                     /locus_tag="IWO89_002892"
     CDS             149..232
                     /locus_tag="IWO89_002892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001814874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA methyltransferase attenuator leader
                     peptide ErmL"
                     /protein_id="HAQ0457579.1"
                     /translation="MLVFQMRYVDKTSTVLKQTKNSDYADK"
     gene            357..1094
                     /gene="erm(B)"
                     /locus_tag="IWO89_002893"
     CDS             357..1094
                     /gene="erm(B)"
                     /locus_tag="IWO89_002893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004292767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2058)-N(6))-methyltransferase
                     Erm(B)"
                     /protein_id="HAQ0457580.1"
                     /translation="MNKNIKYSQNFLTSEKVLNQIIKQLNLKETDTVYEIGTGKGHLT
                     TKLAKISKQVTSIELDSHLFNLSSEKLKLNTRVTLIHQDILQFQFPNKQRYKIVGSIP
                     YHLSTQIIKKVVFESHASDIYLIVEEGFYKRTLDIHRTLGLLLHTQVSIQQLLKLPAE
                     CFHPKPKVNSVLIKLTRHTTDVPDKYWKLYTYFVSKWVNREYRQLFTKNQFHQAMKHA
                     KVNNLSTVTYEQVLSIFNSYLLFNGRK"
BASE COUNT          515 a          221 c          237 g          445 t
ORIGIN      
        1 tcttgcgtat ggttaaccct aaagttatgg aaataagact tagaagcaaa cttaagagtg
       61 tgttgatagt gcattaaaaa ttttgtataa taggaattga agttaaatta gatgctaaaa
      121 atttgtaatt aagaaggagg gattcgtcat gttggtattc caaatgcgtt atgtagataa
      181 aacatctact gttttgaaac agactaaaaa cagtgattac gcagataaat aaatacgtta
      241 gattaattcc taccagtgac taatcttatg actttttaaa cagataacta aaattacaaa
      301 caaatcgttt aacttctgta tttgtttata gatgtaatca cttcaggagt gattacatga
      361 acaaaaatat aaaatattct caaaactttt taacgagtga aaaagtactc aaccaaataa
      421 taaaacaatt gaatttaaaa gaaaccgata ccgtttacga aattggaaca ggtaaagggc
      481 atttaacgac gaaactggct aaaataagta aacaggtaac gtctattgaa ttagacagtc
      541 atctattcaa cttatcgtca gaaaaattaa aactgaatac tcgtgtcact ttaattcacc
      601 aagatattct acagtttcaa ttccctaaca aacagaggta taaaattgtt gggagtattc
      661 cttaccattt aagcacacaa attattaaaa aagtggtttt tgaaagccat gcgtctgaca
      721 tctatctgat tgttgaagaa ggattctaca agcgtacctt ggatattcac cgaacactag
      781 ggttgctctt gcacactcaa gtctcgattc agcaattgct taagctgcca gcggaatgct
      841 ttcatcctaa accaaaagta aacagtgtct taataaaact tacccgccat accacagatg
      901 ttccagataa atattggaag ctatatacgt actttgtttc aaaatgggtc aatcgagaat
      961 atcgtcaact gtttactaaa aatcagtttc atcaagcaat gaaacacgcc aaagtaaaca
     1021 atttaagtac cgttacttat gagcaagtat tgtctatttt taatagttat ctattattta
     1081 acgggaggaa ataattctat gagtcgcttt tgtaaatttg gaaagttaca cgttactaaa
     1141 gggaatgtag ataaattatt aggtataggg cacctctaat aactaccaat taattcacct
     1201 caaaatgaaa ttagaaaaaa acagcgaaat ttttaaatct atttcttatc gatacaaatt
     1261 cctcgtaggc gctcgggacc ccaacatgag gaaatgtttt tataaaaaaa gagtagttac
     1321 cgttttttgg taactactct tttccttttt taaggtttaa atacctggaa gtttaggtgt
     1381 tttgggtatt ggtggtgtag gtggctgcaa gctttcca
//
LOCUS       DACEEZ010000186         1415 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20313_2017
            SAMN12639966-rid9879223.denovo.196, whole genome shotgun sequence.
ACCESSION   DACEEZ010000186 DACEEZ010000000
VERSION     DACEEZ010000186.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639966
            Sequence Read Archive: SRR10023158
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1415)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1415)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741683.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741683.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:44:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,016
            CDSs (total)                      :: 2,962
            Genes (coding)                    :: 2,889
            CDSs (with protein)               :: 2,889
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 36 of 73
            Pseudo Genes (incomplete)         :: 29 of 73
            Pseudo Genes (internal stop)      :: 18 of 73
            Pseudo Genes (multiple problems)  :: 8 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1415
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639966-rid9879223.denovo.196"
                     /isolate="E20313_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            105..1406
                     /locus_tag="IWO89_002894"
     CDS             105..1406
                     /locus_tag="IWO89_002894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HAQ0457581.1"
                     /translation="MPDLHHIKLLLGIKDKNIFITNVESKSIKNIKSLVVSATLSKEI
                     RRCPLCKQMNHEGMIVKNGKKKSLIQLNKCANQLTYLALAKQRYHCRGCHTYFTANTY
                     IVDRNCFIAKQVRYKILEELTEKQAMTTIAKHCGVSWSTVSRTLASLIPMTKVKRNWL
                     PRCLLMDEFRSLKNQVGPYSFSCMDGDTGKLLDILPSRKKKALVSYFMQFERRARLKV
                     KVIVTDMNVSYASLIKECFPKAKLVVDRFHIVKHLIRKFEDIRVRIMKSFDRNDPTQA
                     KYYRQLKALSRLLIKRQDKLVYDKWTKWRNFGWAYLTESEVVDRLLSISDELRIAYFY
                     YQEILQAFHDKEADTFFKLVRTMPNSVPKELHSIKKAFINYESGIRLALELPYSNAKI
                     ENLHTHIKALKRVAYGFRSFRKMKTRIFLLNNLITYESKNI"
BASE COUNT          468 a          284 c          260 g          403 t
ORIGIN      
        1 gatagaaaat cattctatcg gtcccttttg agcatgaaaa aacaaaacat acagaagctc
       61 cgttagaatt gaatcaccac aacacaatca aaggagcgtt ctgtatgcct gatcttcatc
      121 atatcaaact actacttgga atcaaagata aaaacatttt catcacaaat gtggaatcga
      181 agagcattaa gaatatcaag agtcttgttg tttctgcgac gttaagtaaa gaaattcgtc
      241 gctgtccttt gtgtaaacag atgaatcatg aaggcatgat cgttaagaac ggaaagaaaa
      301 aatcgctcat tcaacttaat aaatgtgcga accaactgac ttatcttgct ttagcgaaac
      361 aacgctacca ctgtcgtggc tgccacacct attttacggc gaatacctac attgtcgata
      421 gaaactgctt tatcgctaaa caagtgcgct ataaaatttt agaagagcta actgaaaaac
      481 aagcaatgac gacaattgct aagcattgcg gcgtttcttg gtcgaccgtt tctcggacac
      541 tcgcttctct gatccccatg acaaaagtaa aaagaaattg gttaccgcgt tgtttattaa
      601 tggatgaatt tcgttcactg aaaaatcaag tcggtcccta ctccttttct tgtatggatg
      661 gcgatactgg aaaattactg gatattcttc cttcacggaa gaaaaaggct ctcgtttctt
      721 attttatgca atttgaacga cgcgcacgat tgaaggtaaa ggtcatcgtg acggacatga
      781 atgtttctta tgcctctctg ataaaggaat gttttccaaa ggcaaaactc gttgtggatc
      841 gttttcacat tgtcaaacat ctcattcgta aatttgagga tattcgcgta cgaatcatga
      901 aaagctttga tcgaaacgat ccaacacaag ccaaatatta tcgacaatta aaggcgttgt
      961 ctcggcttct gatcaaacga caggacaagc ttgtttatga caaatggacc aaatggcgca
     1021 attttggttg ggcgtatctc accgaaagtg aagtggtgga tcgtctgctt tcgatctctg
     1081 acgaattacg aatcgcctat ttttactatc aagaaattct tcaagcgttt cacgacaaag
     1141 aggcggacac tttctttaaa cttgtaagaa cgatgcccaa ttccgttcca aaagaactgc
     1201 attctataaa aaaagctttt ataaactatg aatccggcat tcgtttagcc ctcgaattgc
     1261 cttattcaaa tgccaaaata gaaaatctgc atacccacat caaagcattg aaacgagtcg
     1321 cttatggctt tcgaagcttt cggaaaatga agaccagaat tttcttacta aataatctga
     1381 ttacctatga atcaaaaaac atctaaagta gaaaa
//
LOCUS       DACEEZ010000187         1363 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20313_2017
            SAMN12639966-rid9879223.denovo.199, whole genome shotgun sequence.
ACCESSION   DACEEZ010000187 DACEEZ010000000
VERSION     DACEEZ010000187.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639966
            Sequence Read Archive: SRR10023158
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1363)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1363)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741683.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741683.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:44:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,016
            CDSs (total)                      :: 2,962
            Genes (coding)                    :: 2,889
            CDSs (with protein)               :: 2,889
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 36 of 73
            Pseudo Genes (incomplete)         :: 29 of 73
            Pseudo Genes (internal stop)      :: 18 of 73
            Pseudo Genes (multiple problems)  :: 8 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1363
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639966-rid9879223.denovo.199"
                     /isolate="E20313_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            317..859
                     /locus_tag="IWO89_002895"
     CDS             317..859
                     /locus_tag="IWO89_002895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0457582.1"
                     /translation="MLLKVAIKCYCVKNKGGIIYEKIVPRGACLLLALCLSLSAVGCQ
                     KSNENNKIKEGQSISSEEEGMSINANGKNETFKPSDYTLEAKKEYVYEYLGLKFKLSD
                     KFRNYIADKKIAMLDDQSPIDKELKYAILTFEKMTEEQKNAVIEKMGDGYKNWQNELE
                     RIGTIGIFEKNTSEEKNLKL"
     gene            949..>1363
                     /locus_tag="IWO89_002896"
     CDS             949..>1363
                     /locus_tag="IWO89_002896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA family protein disulfide reductase"
                     /protein_id="HAQ0457583.1"
                     /translation="MDELKRTEIQIIDKKERPKNGFVLSEKTDLENTEAFNKESVKDL
                     RKLPTKDINGKDFTSKDFEKYDLTMVNVFATWCTACVKEIPDLVEVQNEMKSKGVNIV
                     GVVTDPVDDNGENKEAIEKSKLIHEKTKASYPFLMP"
BASE COUNT          540 a          162 c          256 g          405 t
ORIGIN      
        1 tttgaattct tttgaatagg ttcttcgttg acgtcttgac atgaaaaatc ctccagtgtg
       61 ttttttatta ttctacacac cttatttttt ctgtctagtc tagtgtagcc gattcaaatt
      121 gatgaagcag atcccaaagg gctgcttcat caattttttc gtaagtgtcc gtacacttac
      181 tgtgcttgct catatattaa tcgatcatat actaatcgtg taagaacaac tttaagcata
      241 atacttatat tgttatacca tagtttggaa aaataaaaaa ttatatttca tcaacgtttc
      301 ataaaaggtg tgtagaatgt tattgaaagt agctataaaa tgctattgtg taaaaaataa
      361 aggaggaata atttatgaaa aaatagtacc aagaggagca tgcctattac ttgcattatg
      421 tttgagctta agcgcagttg ggtgtcaaaa aagtaatgaa aacaataaaa taaaagaagg
      481 acaatccata agttctgaag aggaaggcat gtcaattaat gcaaatggca aaaatgaaac
      541 ttttaagcca tcagattata cattggaggc aaagaaagaa tatgtttatg aatatttagg
      601 gcttaaattt aaactctcag acaaatttag aaattatata gctgataaaa agatagcgat
      661 gttagatgat caaagtccta tagataaaga actaaagtat gctattttga catttgagaa
      721 aatgacagaa gaacaaaaga atgcagttat agaaaagatg ggcgatggat ataaaaattg
      781 gcaaaatgag cttgaaagaa ttgggactat tggtatattt gaaaaaaata catcagaaga
      841 aaaaaatcta aaattataaa atgtgatact catactaaaa taggagtttc aagtgatgga
      901 aaatacgatt gctatttaag cacaaatagc ggagctgaaa gtaatcttct ggatgaatta
      961 aaaagaactg agattcaaat tatagataaa aaagaacgtc ctaaaaatgg ttttgtgcta
     1021 tcagaaaaaa ctgatttaga aaatacagaa gcatttaaca aagaatcggt taaagattta
     1081 cgtaaactac caacaaagga cataaatggc aaggatttta caagtaaaga ttttgagaag
     1141 tatgatctta ctatggtaaa tgtatttgca acttggtgta cagcttgtgt aaaggaaatt
     1201 cctgatttgg ttgaggttca gaatgaaatg aaaagcaagg gtgtaaatat cgttggagtt
     1261 gttacagatc ctgttgatga taacggtgaa aacaaggaag caattgaaaa gtcaaagtta
     1321 atacatgaaa agacaaaggc ttcatatcct ttcttaatgc cag
//
LOCUS       DACEEZ010000188         1314 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20313_2017
            SAMN12639966-rid9879223.denovo.200, whole genome shotgun sequence.
ACCESSION   DACEEZ010000188 DACEEZ010000000
VERSION     DACEEZ010000188.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639966
            Sequence Read Archive: SRR10023158
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1314)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1314)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741683.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741683.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:44:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,016
            CDSs (total)                      :: 2,962
            Genes (coding)                    :: 2,889
            CDSs (with protein)               :: 2,889
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 36 of 73
            Pseudo Genes (incomplete)         :: 29 of 73
            Pseudo Genes (internal stop)      :: 18 of 73
            Pseudo Genes (multiple problems)  :: 8 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1314
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639966-rid9879223.denovo.200"
                     /isolate="E20313_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            <1..848
                     /locus_tag="IWO89_002897"
     CDS             <1..848
                     /locus_tag="IWO89_002897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000997693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="HAQ0457584.1"
                     /translation="EIADLIEKMYGHHYTPQTMSNITKSFTEEVTAFKGRELHDRYAA
                     IYMDATYIPLKRKTVAKEAIHIAVGIRPDGSKEVLSYAIAPTESITIWEEILLDLQER
                     GLKNVLLFITDGLKGMVGAISRFYPKARFQHCCVHVFRNISHKVRVNNRKEVCDDFKM
                     VYQASSKEVALEARGAFAKKWKTSYPKVVESILSNDHLLTFYDFPLAIRKSIYSTNLI
                     ESFNKQIKKYSHRKEQFQNEESMERFLVSSFDTYNQKFLGRSHKGFQQAEGELEQMLS
                     QPMEN"
BASE COUNT          429 a          240 c          254 g          391 t
ORIGIN      
        1 ctgaaattgc tgatttgatc gaaaaaatgt acggtcatca ctatactcca caaaccatgt
       61 ccaatataac aaaatcattt acagaagagg taacggcgtt taaagggcga gagcttcatg
      121 accgttatgc tgctatttat atggacgcaa cgtatattcc gttaaagcgg aaaaccgtcg
      181 ccaaggaagc tattcatatc gcagttggca ttcgcccgga cggatcaaag gaagtattga
      241 gctatgcgat tgcaccgact gaatccatca cgatttggga ggaaatttta ttagaccttc
      301 aagagcgcgg tttgaaaaat gtcctcctgt tcatcacgga tggcttaaag gggatggtag
      361 gagcgattag tcggttctat cccaaagctc gttttcaaca ttgttgtgta cacgtttttc
      421 gtaatatcag tcacaaagtg cgtgtcaata atcgtaagga agtctgtgat gattttaaaa
      481 tggtgtatca agcctcatct aaagaggtgg cgttagaagc acgtggtgct tttgcgaaaa
      541 aatggaaaac cagctatcca aaagtggttg aatcgattct ttcgaacgat cacttgctca
      601 ctttctatga tttccccttg gccatacgca agagtattta ttctacgaac ttgattgaat
      661 cctttaataa gcaaatcaag aaatacagcc accgcaagga acagttccaa aacgaagagt
      721 cgatggaacg tttcttagtc tcgtcttttg atacttacaa ccaaaaattc ctaggtcgca
      781 gtcataaagg tttccaacag gccgaaggcg agctcgaaca aatgctgagt caaccgatgg
      841 agaattagaa acaatctaca gtagggaaga accatttaca caaaattatt gacgctccca
      901 agctaaaact cctattaatc gatctactaa aaataaataa aaaaattctt ttcaattttt
      961 atttatggta tgacataaat aaagtaaagt tattcttcta ggttaatctt attaatactc
     1021 tttctcacta gtgttgcatg caacactagt gagaaagagt attaaatata ctgcaaggca
     1081 agacatttgg taattgaaga ataacaagca aaaaaatagc agtcaggtct cttgacaaag
     1141 tatagttgct attctttttt taggccctac tttaatgaaa tttttgtttg gagggaacga
     1201 cttcattcct ttgattattt cagttgaatt taatagagaa gcctggcgtc tattaagaaa
     1261 tacttttctt gatgagcgtc aattttttta acggaaatct atcctatact aaat
//
LOCUS       DACEEZ010000189         1302 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20313_2017
            SAMN12639966-rid9879223.denovo.201, whole genome shotgun sequence.
ACCESSION   DACEEZ010000189 DACEEZ010000000
VERSION     DACEEZ010000189.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639966
            Sequence Read Archive: SRR10023158
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1302)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1302)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741683.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741683.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:44:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,016
            CDSs (total)                      :: 2,962
            Genes (coding)                    :: 2,889
            CDSs (with protein)               :: 2,889
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 36 of 73
            Pseudo Genes (incomplete)         :: 29 of 73
            Pseudo Genes (internal stop)      :: 18 of 73
            Pseudo Genes (multiple problems)  :: 8 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1302
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639966-rid9879223.denovo.201"
                     /isolate="E20313_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            52..>1302
                     /locus_tag="IWO89_002898"
     CDS             52..>1302
                     /locus_tag="IWO89_002898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HAQ0457585.1"
                     /translation="MSHTQLIKDTLNILDLNIYFEENCLTKEKYKGQICLIYKGTLLY
                     KPEECSHCLCVVPSRIIRWGTTTVRLLLNDVSEYRTYLELKKQRFKCKSCQRTFVADT
                     SVAEKHCFISQKVRWSVIARLKENTSMTEIARQKNISTSSVYRVMKRFYRPLNPFKQT
                     LPKVLCFDEFKSVRSVASAMSFIMMDGQSHALLDIVENRRLPYLERYFSRFSLATREA
                     VQLIVIDMYAPYVSLVKKLFPNAQLIIDRFHIVQHIGRTFLNHRVKETTVRLKGNSHS
                     QRGLGKKLKRYWKLLQKEEAKLNYEKRVWRASFKDYLTESEIADRLLAACPNLRQGYQ
                     LYQDILYAVKKRDQSLFEDCLTRDISGLPETYTTTLRTFKKFLPQIQNALHYSYSNGP
                     LECLNNHIKVLKRNAYGFRNFYNFK"
BASE COUNT          403 a          260 c          252 g          387 t
ORIGIN      
        1 cctgaaatcc tttagaatta atttggctac caattcatag gaggattcaa gatgtctcat
       61 actcagctta tcaaagatac cctaaatatt ctagacctaa atatctattt cgaggaaaat
      121 tgtttaacta aagaaaaata caaagggcaa atttgcttga tttacaaagg aactcttctg
      181 tacaagccag aagagtgttc ccattgtctt tgtgtcgtcc cctctaggat cattcgttgg
      241 gggacgacaa ccgttcggtt gttactgaat gatgtgtctg agtatcgcac ttatctggag
      301 ctgaagaaac aacgatttaa atgtaagtct tgccaaagaa cattcgtagc ggatacctct
      361 gttgctgaaa aacactgttt cattagtcaa aaagttcgtt ggtctgtcat tgctcgctta
      421 aaagagaata cttccatgac ggaaatcgct cgtcagaaaa atatttccac ttcttcggtc
      481 tatcgtgtca tgaagcgatt ctaccgccca ttgaatcctt tcaaacagac tttgccaaag
      541 gtcttatgct ttgatgaatt caaatcggtt cgaagtgttg ccagtgcaat gagttttatc
      601 atgatggacg gacaaagcca tgcattattg gatattgtcg aaaatcgacg cttgccttat
      661 ttagaacgct atttttctcg gttttcatta gcgactcgtg aagccgttca attgattgtt
      721 attgatatgt atgcgcctta tgtttcgttg gtgaaaaagc ttttcccgaa tgctcagttg
      781 atcatcgatc gtttccacat cgtccaacat attggcagaa ctttcttgaa tcatcgagtg
      841 aaagaaacca cggtacgact taaaggaaat tcccactctc aacgagggtt agggaaaaaa
      901 ttgaagcgct attggaaact cctacaaaaa gaagaagcaa agttaaacta tgaaaaacgg
      961 gtttggcgtg ccagttttaa agattatttg actgaaagtg agattgccga tcgactgtta
     1021 gctgcttgtc ccaacttacg ccaaggctat cagttatacc aagacattct ttatgcggta
     1081 aagaaaagag atcagtcact atttgaggat tgtttaacta gagacattag cggtctgcca
     1141 gaaacctata caaccacttt acgaacgttt aaaaagtttc tgccacagat ccaaaatgcg
     1201 cttcactaca gctattcaaa cggaccattg gaatgcttaa acaaccacat caaagtatta
     1261 aaacgcaatg cgtacggctt tcgcaacttt tacaatttca aa
//
LOCUS       DACEEZ010000190         1297 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20313_2017
            SAMN12639966-rid9879223.denovo.202, whole genome shotgun sequence.
ACCESSION   DACEEZ010000190 DACEEZ010000000
VERSION     DACEEZ010000190.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639966
            Sequence Read Archive: SRR10023158
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1297)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1297)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741683.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741683.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:44:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,016
            CDSs (total)                      :: 2,962
            Genes (coding)                    :: 2,889
            CDSs (with protein)               :: 2,889
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 36 of 73
            Pseudo Genes (incomplete)         :: 29 of 73
            Pseudo Genes (internal stop)      :: 18 of 73
            Pseudo Genes (multiple problems)  :: 8 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1297
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639966-rid9879223.denovo.202"
                     /isolate="E20313_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            141..398
                     /locus_tag="IWO89_002899"
     CDS             141..398
                     /locus_tag="IWO89_002899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0457586.1"
                     /translation="MSNLENKEEKVVNKIVSVVNKLDKELDELDTLSENPEKKHNLKK
                     WLVERKAIHEIKKVLHEADKYEKYDEKELDKEFKEINDLLL"
     gene            complement(630..1043)
                     /locus_tag="IWO89_002900"
     CDS             complement(630..1043)
                     /locus_tag="IWO89_002900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014834562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4231 domain-containing protein"
                     /protein_id="HAQ0457587.1"
                     /translation="MDQKAFIESIDSTIDRLKKEIRFYNRVIGIGNIIKIVLSASIPI
                     LIDQASEHRSLLLIVSIASAIITIIQSGMSAFNYQDKVQTSTELLMKIEKEKLLYITK
                     TSPYNKTDEENFHLIVTTLQTELNDIISDFNQVNN"
BASE COUNT          490 a          165 c          162 g          480 t
ORIGIN      
        1 ttctgtccac gttcatttta ctccaaagtt ttcctcattt caaaagaaaa tcacagaata
       61 cacgaagaag cgtttctatt tgtataaact atatcaaagt tctatactta aattatcaaa
      121 taaaaaggac gtgaagataa atgtcaaatt tagaaaacaa agaagaaaaa gttgtaaaca
      181 aaattgtttc tgttgtcaat aaactggata aagagttaga cgaacttgat acgttatctg
      241 aaaatccaga aaaaaaacat aacctaaaaa aatggttagt tgaacgaaag gctattcatg
      301 aaattaaaaa agttcttcat gaagctgata aatatgaaaa atatgatgaa aaagaacttg
      361 ataaagaatt taaagaaatc aatgacttat tactataatt gatttatacg actcattttt
      421 gagtcgtcag actgcaaaca acactctaaa tttaggagtt tgtctacagt ctgaatagat
      481 tttaaattta ttgtccataa tgttattatg aatttatagc attaagaaaa gagataagac
      541 tttacatttt tttaggaatg taaagtctta tctcttttct taattgtaca attagtatta
      601 tctgcactac tttctaatta aataaatttt tagttattaa cttgattaaa atcagagata
      661 atatcattta attcagtttg aagggttgta acgattaaat ggaaattttc ttcatctgtt
      721 ttattatatg gggatgtttt cgttatatat aacaactttt ctttttcaat tttcattaat
      781 aactctgtcg aagtctgtac tttatcttga taattaaatg cagacatacc actttgtata
      841 attgtaatta ttgctgaagc aattgaaaca atcaatagta aagacctgtg ttcggaagcc
      901 tgatcaatta atattgggat actagctgat aagactattt ttataatatt tccgatacca
      961 attactctat tataaaatcg aatttctttt tttaatctat cgattgtaga atcaatagat
     1021 tcgataaacg ctttttgatc catcattttc tctccaatca ttttattaaa atcattaaca
     1081 aaaatattat aataggattt aagtaagttc taactaatca catataaaaa taaaaaaaca
     1141 tttgtatttt atcaaagttt tattatactt gtcaatttgt gtacatccta aaatataagc
     1201 tatttgttta cttctgtaga atatatactt tgttgttaga taagttgcac atgtttatag
     1261 aatctaaata ataaattttt atgtaccgcc cctcaaa
//
LOCUS       DACEEZ010000191         1257 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20313_2017
            SAMN12639966-rid9879223.denovo.203, whole genome shotgun sequence.
ACCESSION   DACEEZ010000191 DACEEZ010000000
VERSION     DACEEZ010000191.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639966
            Sequence Read Archive: SRR10023158
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1257)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1257)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741683.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741683.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:44:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,016
            CDSs (total)                      :: 2,962
            Genes (coding)                    :: 2,889
            CDSs (with protein)               :: 2,889
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 36 of 73
            Pseudo Genes (incomplete)         :: 29 of 73
            Pseudo Genes (internal stop)      :: 18 of 73
            Pseudo Genes (multiple problems)  :: 8 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1257
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639966-rid9879223.denovo.203"
                     /isolate="E20313_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            12..1091
                     /locus_tag="IWO89_002901"
     CDS             12..1091
                     /locus_tag="IWO89_002901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HAQ0457588.1"
                     /translation="MTQTHDNTVSRKGKHLSYSERCQIAILKKEGYSNRQVAKVLGRV
                     PQTINNEIDRGTITQLKRQTQNGKVYDTYYSVYDADAGQAFYEKQRLNCGRRPKWANT
                     NAFIDWADDKMLNEKWSPDVVVGFARKHELFDCAIIPGTTALYQWIDRGIMRTKNMDL
                     LEKLSRKIKAVRPGHRPNKRVLGTSIEERPKEVETRETFGHWEIDTVVGNKTKSDAVL
                     LTLAERQTRFEVILKLNGKDTESVDQAVQQLRERAGANFDQLFKTVTSDNGSEFSGLH
                     DALQDVADVYFSHPYASWERGTSENQHKLIRRFVPKGSPISEVSENQILRIQRWMNDY
                     PRKILGYATPHDTFVQAFKQERLVA"
BASE COUNT          372 a          300 c          308 g          277 t
ORIGIN      
        1 gatgaattca catgacgcaa acccatgata acacagtttc acgcaaggga aaacatcttt
       61 cctactcaga acgttgccaa atcgctatcc tgaaaaagga aggctattcc aaccgtcagg
      121 ttgccaaagt gctgggacgt gttccgcaaa ccataaacaa tgagattgat cgcggaacca
      181 tcacccagtt aaaacgccaa acgcaaaatg gaaaagtgta tgacacctac tattctgttt
      241 atgacgctga tgcaggacag gccttctatg aaaagcaacg actgaactgt ggcagacgcc
      301 ccaaatgggc gaacacaaat gccttcattg attgggcaga tgacaaaatg ttaaatgaaa
      361 aatggtcgcc ggacgtcgtg gtcggatttg ccagaaaaca cgagctgttc gattgtgcca
      421 tcattcctgg cacaaccgct ctttatcaat ggattgaccg cggaatcatg cgaacaaaga
      481 acatggactt gcttgaaaaa ctgtcccgta aaatcaaagc ggtccgccca ggacaccgtc
      541 ccaacaagcg tgttctcggg acgtcaattg aagaacgccc taaagaagtt gaaacacggg
      601 aaacgttcgg tcattgggaa atcgacacgg tagtgggaaa caagaccaag tcggatgccg
      661 tgctgctgac gctggctgaa cggcagacac gctttgaagt catcctaaag ttgaacggga
      721 aggacaccga atcggtggac caagcggttc aacagctaag agaacgggct ggcgctaact
      781 tcgatcaatt atttaagacc gttacatccg acaatggctc ggagttttct gggctacacg
      841 atgctctgca agatgtagct gacgtctatt tcagccaccc ctatgcttct tgggagcgtg
      901 ggacgagcga aaaccagcac aaattaattc gccgtttcgt ccccaaggga agcccgatta
      961 gtgaggtgag cgagaaccag attttgcgga tccagcgttg gatgaacgat tacccgcgga
     1021 aaattttggg ttatgcgaca ccacatgata cattcgtcca ggcattcaag caagaacggt
     1081 tggtggcttg acccattatt ccttgataac gatcctcact cgggacatgg cttcatgtct
     1141 ggaaagctgg cctggggcca gaatcgtctg aagactatca tacctcagtt cattatcaag
     1201 gcctcactga tttagaataa cgaggcagtt acggtatcaa acaaatgagt tacctgt
//
LOCUS       DACEEZ010000192         1253 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20313_2017
            SAMN12639966-rid9879223.denovo.204, whole genome shotgun sequence.
ACCESSION   DACEEZ010000192 DACEEZ010000000
VERSION     DACEEZ010000192.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639966
            Sequence Read Archive: SRR10023158
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1253)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1253)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741683.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741683.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:44:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,016
            CDSs (total)                      :: 2,962
            Genes (coding)                    :: 2,889
            CDSs (with protein)               :: 2,889
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 36 of 73
            Pseudo Genes (incomplete)         :: 29 of 73
            Pseudo Genes (internal stop)      :: 18 of 73
            Pseudo Genes (multiple problems)  :: 8 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1253
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639966-rid9879223.denovo.204"
                     /isolate="E20313_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            12..1091
                     /locus_tag="IWO89_002902"
     CDS             12..1091
                     /locus_tag="IWO89_002902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006908283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HAQ0457589.1"
                     /translation="MTQNHDNTVSRKGKHLTYSERCQIAILKKENYSNRQVADVLGCV
                     PQTINNEIKRGTVTQLNRQNQNGKVYDYYHDVYDADAGQAAYEKNRLNCGRRPLWADT
                     DAFIEWADDMMLLEKWSPDVVVGFARKHELFDPAIIPGTTTLYQWIDRGIMKTKNMDL
                     LEKLSRKPKSSRHGNRPNKRVLGKSIEERPEAVGTRKTFGHWEVDTVVGNKTKSDAVL
                     LTLVERQTRFEVILKLAGKDAESVNQAVRQLRERAGAYFGQLFKTVTSDNGSEFAGLH
                     DALQDVANIYFSHPYASWERGTSENQHKLIRRFIPKGSPIGNVSENQILRIQRWMNDY
                     PRKILGYATPHDTFVQAFRQERLTA"
BASE COUNT          329 a          341 c          354 g          229 t
ORIGIN      
        1 gatgaattca catgacgcaa aaccatgata acacagtttc acgcaaggga aaacacctaa
       61 cctactcaga acgttgccaa atcgccatcc tgaaaaagga aaactattcc aaccgtcagg
      121 ttgcggatgt cctgggctgc gtgccgcaga ccataaacaa cgaaatcaaa cgcgggaccg
      181 ttactcagct taaccgccag aatcagaacg ggaaggtcta cgactattat catgacgtct
      241 acgacgccga tgccgggcag gcggcctacg agaagaaccg cttgaactgc ggcagacggc
      301 ccttatgggc cgacacggac gccttcattg aatgggcgga cgacatgatg ctgttggaaa
      361 aatggtcccc ggacgtggtg gtgggctttg ccagaaaaca cgaattgttc gacccggcca
      421 ttatccctgg gaccaccacc ctctatcagt ggatcgaccg tggcatcatg aaaacgaaga
      481 atatggacct gctagaaaaa ctgtcccgca aaccgaaatc gtcccgtcat ggcaaccgcc
      541 cgaataaacg ggtgctaggg aaatccattg aagagcgccc ggaggcggtt ggtacccgga
      601 aaaccttcgg ccattgggaa gtcgacacgg tcgtaggaaa caagaccaag tcggatgccg
      661 tgctgctgac gttggtggaa cggcagacac gcttcgaggt cattctgaag ctggccggga
      721 aggatgccga atcggtcaac caagcggtcc gacagctgag agaacgggct ggtgcgtact
      781 tcggccagct gttcaagacg gttacctcgg acaacggctc ggagttcgcg ggactgcacg
      841 atgccttgca ggatgtggca aacatctact tcagccaccc ctatgcttcc tgggagcgcg
      901 ggacgagcga aaaccaacac aagctgattc gccgtttcat ccccaagggc agcccaatcg
      961 gcaacgtgag cgaaaaccaa atcttgcgga ttcagcgttg gatgaacgat tacccgcgaa
     1021 agattttggg ctatgcaacg ccgcatgaca cgttcgtcca ggcatttagg caggaacggt
     1081 tgacggcttg acacgtgggc ccttgacaac gagcctccct cggcatggct tgtgtctgga
     1141 aagctggccg gggccagcca gtcgggagac taccatgcct cagctcattg tcaagggccc
     1201 actcgattag aaaaattaga cggttggtgg taacgagcaa ccgggtcacc atg
//
LOCUS       DACEEZ010000193         1245 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20313_2017
            SAMN12639966-rid9879223.denovo.205, whole genome shotgun sequence.
ACCESSION   DACEEZ010000193 DACEEZ010000000
VERSION     DACEEZ010000193.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639966
            Sequence Read Archive: SRR10023158
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1245)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1245)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741683.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741683.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:44:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,016
            CDSs (total)                      :: 2,962
            Genes (coding)                    :: 2,889
            CDSs (with protein)               :: 2,889
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 36 of 73
            Pseudo Genes (incomplete)         :: 29 of 73
            Pseudo Genes (internal stop)      :: 18 of 73
            Pseudo Genes (multiple problems)  :: 8 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1245
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639966-rid9879223.denovo.205"
                     /isolate="E20313_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            <1..1244
                     /locus_tag="IWO89_002903"
     CDS             <1..1244
                     /locus_tag="IWO89_002903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS66 family transposase"
                     /protein_id="HAQ0457590.1"
                     /translation="ITEKEHLLPLEEQFCEHCGSQMKDIGRTKVREEIRFHQAMLDCL
                     THYQHTYCCKSCEKEGLSSFKKAIVPKPLISNSLGSNSLVAETIRMKFGQKVPAYRQE
                     NYWKQTHGLDISRDNITNWHIKAVQNALDPLGERLRVYLNQEEILHGDETSYRVIESA
                     KTDTYYWQFCTGKDSQHPIVYYHHDESRAGDVPKTFLKEFTGYLHCDGYSGYNAVESV
                     RLVYCFAHVRRKFFEAIPKGKKNTDIPAAQAVKQLDKWFVLEKKWKDFSPEKRLSCRQ
                     QELRPLFIAFYEWMATIDPVAKSKLDAAVQYACKLRSGFEPIFEDGRLELTNNRAERN
                     IKELVIGRKNWLHSTSLEGARTSGIILSVYKTAELNGLNPVKYLEFLFDKIPNLPVLS
                     AETLDQLLPWNKDVQQHFSRN"
BASE COUNT          410 a          222 c          268 g          345 t
ORIGIN      
        1 ctataacaga gaaagaacat ctccttcctt tggaagaaca attttgtgaa cattgcggtt
       61 cacagatgaa agatatcgga cggacaaaag tacgagaaga aattcgtttc catcaggcta
      121 tgttggattg tctcacccat tatcaacata cctattgttg taaaagctgc gaaaaagaag
      181 gactttcttc ttttaaaaaa gcgatcgttc ctaaaccttt gatttctaat agcttaggtt
      241 ccaatagttt agtggcggaa acgatccgga tgaaatttgg tcagaaagtt cctgcctatc
      301 ggcaagagaa ctattggaaa caaacacatg ggttggatat ttcgagagat aatatcacca
      361 attggcatat taaagcagtg caaaacgcgt tagacccttt aggagaacga ttaagagtct
      421 atcttaatca agaagagatt ctacacgggg atgaaacaag ttatcgtgtg attgaaagtg
      481 ccaaaacaga tacctattac tggcagtttt gtacaggaaa agacagtcag catcccatcg
      541 tttattatca ccacgacgaa agtcgtgctg gagatgttcc gaaaactttt ctcaaggagt
      601 ttacagggta tttacactgt gacggctata gcgggtataa tgccgtggaa agtgtacgac
      661 ttgtctattg tttcgcgcat gttcggcgga aatttttcga agcaattcca aaaggaaaga
      721 aaaacacgga tattcccgca gctcaagcag tcaaacaatt ggataaatgg tttgtcctag
      781 aaaagaaatg gaaagacttt tctcctgaga agcgattaag ctgtcggcaa caagaactgc
      841 gtccattatt tattgctttt tatgagtgga tggcaacgat agatcctgtt gcaaaatcca
      901 agttggatgc agccgttcaa tatgcgtgta aactacgaag tggttttgaa cctatttttg
      961 aagatggtcg tttagaactc acaaataatc gtgcggagcg aaatatcaaa gagctggtga
     1021 taggacggaa aaattggttg cattccacaa gtcttgaagg agcacgaact tcaggaatca
     1081 ttctgagtgt atataaaaca gcagaactta acggattgaa tcctgtaaag tatctagagt
     1141 ttctatttga taagatacca aatcttcccg tactttcagc agaaacttta gatcaactgt
     1201 tgccttggaa caaggatgta caacaacatt tttctcgtaa ctaaa
//
LOCUS       DACEEZ010000194         1215 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20313_2017
            SAMN12639966-rid9879223.denovo.206, whole genome shotgun sequence.
ACCESSION   DACEEZ010000194 DACEEZ010000000
VERSION     DACEEZ010000194.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639966
            Sequence Read Archive: SRR10023158
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1215)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1215)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741683.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741683.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:44:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,016
            CDSs (total)                      :: 2,962
            Genes (coding)                    :: 2,889
            CDSs (with protein)               :: 2,889
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 36 of 73
            Pseudo Genes (incomplete)         :: 29 of 73
            Pseudo Genes (internal stop)      :: 18 of 73
            Pseudo Genes (multiple problems)  :: 8 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1215
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639966-rid9879223.denovo.206"
                     /isolate="E20313_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            414..947
                     /locus_tag="IWO89_002904"
     CDS             414..947
                     /locus_tag="IWO89_002904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease"
                     /protein_id="HAQ0457591.1"
                     /translation="MNFVAMDFETANHQPYSACSLALVMVKNSQIVDEFYTLIQPETP
                     FFWRNVQIHGIHQEDVRNAPKFPEVWQTIQPYFQKNRLVVAHNAAFDTKVLAGCLDYY
                     HLEQPNYLSLCTVKSSRRLFPEFPNHRLNTVCENLNIQLDHHHDALEDSRACAEILLY
                     QEKKFGTDPLKKLVTIK"
BASE COUNT          389 a          226 c          225 g          375 t
ORIGIN      
        1 cttattaccg cagatgcaag cttttgaaca ccgtttattg agaagttgtg aaatcagcgt
       61 cctgacctga gtattagaga aacaaatggc aaaatagttc ctcatatttt gctatttttt
      121 gagctaatac cgagggtcag ctgattgaac accgtttagt ggagaagttg tgaaaaagtc
      181 gcctactttc gagtcgtaag aaattttttt gaaaatggtt cctcacaact ttgcataaat
      241 ttgacttaat accgagaagt catgatcccc atctaactcc aaatcacaag gaatgagttc
      301 aaaaactagc ttttctcaac aaatattaca taaaaaattc tatgtttttt gttaaattta
      361 tttgcttttt tcaaaagtat gttacagtaa tttcccaagg aggttttaca gaaatgaatt
      421 ttgttgccat ggattttgaa actgccaatc atcagcctta tagtgcttgt tctcttgcgc
      481 tagtcatggt aaaaaatagc cagatcgttg atgaattcta tacattgata cagcctgaga
      541 ctcctttttt ctggcgaaat gtccaaattc acggaatcca tcaagaagac gtacgcaatg
      601 cgccgaaatt cccagaagtc tggcaaacta tccaaccata ttttcaaaaa aatcgtttag
      661 tcgttgcaca taatgctgct tttgatacaa aggtcttagc gggatgctta gattattatc
      721 atcttgaaca gcctaattat ctttctttat gtacagtgaa aagtagccgt cgcctgtttc
      781 ctgaatttcc aaatcatcga ttgaacactg tgtgcgaaaa tttgaacatc caattagatc
      841 atcaccatga tgcattggaa gatagtcgtg catgtgcgga aatcctgctt tatcaagaaa
      901 aaaaattcgg gacagatcca ttgaaaaaac ttgtgactat aaagtaaaag aatacttatg
      961 aaacacacaa agaggtcgtg agcctgccgt ttcactccga gtcacaagga gcaatttcaa
     1021 aaaatagctt ctcatgttta ttaaatattg tgactggtga ccgaggagct ggcaggagaa
     1081 caccgtttat tggagaggtt gtgaaaaagc tgttctgcat caagtaataa gaacagccaa
     1141 acaaaaatag cttctcatat ttttcatttg gttggactta ttaccgcaga tgcaagcttt
     1201 tggacaccgt ttacc
//
LOCUS       DACEEZ010000195         1194 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20313_2017
            SAMN12639966-rid9879223.denovo.207, whole genome shotgun sequence.
ACCESSION   DACEEZ010000195 DACEEZ010000000
VERSION     DACEEZ010000195.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639966
            Sequence Read Archive: SRR10023158
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1194)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1194)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741683.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741683.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:44:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,016
            CDSs (total)                      :: 2,962
            Genes (coding)                    :: 2,889
            CDSs (with protein)               :: 2,889
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 36 of 73
            Pseudo Genes (incomplete)         :: 29 of 73
            Pseudo Genes (internal stop)      :: 18 of 73
            Pseudo Genes (multiple problems)  :: 8 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1194
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639966-rid9879223.denovo.207"
                     /isolate="E20313_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(<1..>1194)
                     /gene="aph(2'')-I"
                     /locus_tag="IWO89_002905"
     CDS             complement(<1..>1194)
                     /gene="aph(2'')-I"
                     /locus_tag="IWO89_002905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012655884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="APH(2'')-Ia/If/Ih family aminoglycoside
                     O-phosphotransferase"
                     /protein_id="HAQ0457592.1"
                     /translation="TEPWEDEVFRVIIEYNNVPIGYGQIYKMYDELYTDYHYPKTDEI
                     VYGMDQFIGEPNYWSKGIGTRYIKLIFEFLKKERNANAVILDPHKNNPRAIRAYQKSG
                     FRIIEDLPEHELHEGKKEDCYLMEYRYDDNATNVKAMKYLIEHYFDNFKVDSIEIIGS
                     GYDSVAYLVNNEYIFKTKFSTNKKKGYAKEKAIYNFLNTNLETNVKIPNIEYSYISDE
                     LSILGYKEIKGTFLTPEIYSTMSEEEQNLLKRDIASFLRQMHGLDYTDISECTIDNKQ
                     NVLEEYILLRETIYNDLTDIEKDYIESFMERLNATTVFEGKKCLCHNDFSCNHLLLDG
                     NNRLTGIIDFGDSGIIDEYCDFIYLLEDSEEEIGTNFGEDILRMYGNIDIEKAKEYQD
                     IVEEYY"
BASE COUNT          395 a          209 c           93 g          497 t
ORIGIN      
        1 ataatattct tcaactatat cttgatattc ttttgctttc tcaatatcta tatttccata
       61 cattcttaat atatcttctc caaaatttgt tcctatttct tcttcactat cttcaagtaa
      121 gtatataaaa tcacaatatt catctataat tccagaatct ccaaaatcaa ttattccagt
      181 taatctatta ttgccatcta acaatagatg attacaacta aaatcattat ggcataaaca
      241 ctttttaccc tcaaaaactg ttgttgcatt tagtctttcc ataaaacttt ctatataatc
      301 tttttctata tcagttaaat cattataaat agtttcacgc aacaatatat actcttctaa
      361 tacattttgt ttattatcaa tagtacattc actaatatct gtataatcta aaccgtgcat
      421 ttgtcttaaa aaactggcaa tatctcgttt taacaaattt tgttcttctt ctgacatagt
      481 agaataaatt tctggtgtta aaaaagttcc tttaatttct ttataaccta gtatagataa
      541 ttcatcacta atatacgaat attcaatatt aggaattttt acattagttt ctaaatttgt
      601 atttaaaaaa ttatatattg ctttttcttt tgcataacct tttttcttat tagtactaaa
      661 ttttgtttta aaaatgtatt cattattaac taaatatgcc acactatcat aaccactacc
      721 gattatttca atactatcta ctttgaaatt atcaaagtaa tgctcaatta aatatttcat
      781 tgccttaaca tttgtggcat tatcatcata tctatattcc attaaataac aatcttcttt
      841 tttgccctcg tgtaattcat gttctggcaa atcttcaata attctaaaac cagatttttg
      901 gtatgccctt attgctcttg gattattttt atgagggtct aaaataactg cattagcatt
      961 tctttctttt ttcaaaaatt caaaaatcaa tttaatatat cttgtaccaa ttcctttact
     1021 ccaataattt ggctctccta taaattgatc cataccatag actatctcat cagtttttgg
     1081 ataatgataa tcagtatata actcatcata cattttatat atttgtccat atccaatagg
     1141 aacattgtta tattcaataa ttactctaaa aacttcatct tcccaaggct ctgt
//
LOCUS       DACEEZ010000196         1193 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20313_2017
            SAMN12639966-rid9879223.denovo.208, whole genome shotgun sequence.
ACCESSION   DACEEZ010000196 DACEEZ010000000
VERSION     DACEEZ010000196.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639966
            Sequence Read Archive: SRR10023158
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1193)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1193)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741683.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741683.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:44:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,016
            CDSs (total)                      :: 2,962
            Genes (coding)                    :: 2,889
            CDSs (with protein)               :: 2,889
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 36 of 73
            Pseudo Genes (incomplete)         :: 29 of 73
            Pseudo Genes (internal stop)      :: 18 of 73
            Pseudo Genes (multiple problems)  :: 8 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1193
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639966-rid9879223.denovo.208"
                     /isolate="E20313_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            <1..1076
                     /locus_tag="IWO89_002906"
     CDS             <1..1076
                     /locus_tag="IWO89_002906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014748673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HAQ0457593.1"
                     /translation="VIGMESTSMYSFHPSMFFHEDEKLKKLNTLVTIENPFRVKQFSR
                     MFDENKTDRNDALRIADFLRIQRFTTSPIKEEKYMALQRLTRTRYQLIKQLIRTKQHF
                     LENLTYKCNTLAREMRDESTSLFSATLISLMTEDFTLDELAELPLEAFCDLLQEKGKG
                     RFKQPEKIAKAIQRAITMSYRLGALAQESINVVLSVLVREIRALEKNIKELDKAIEQV
                     IVVIPEYQCLTSIPGVGKVYAAGLIAEIGQIERFEDQTKLAKYAGLSWKVNQSGNYQS
                     QNTPLTKQGNRYFRYYLVEAANSVKNYLPEYKAFYQSKYKEVPKHQHKRALVLTARKF
                     TRLVDTLLRKHQLYTPPRSVIEK"
BASE COUNT          418 a          234 c          210 g          331 t
ORIGIN      
        1 ttgtgattgg catggagtca acgtccatgt acagcttcca tccttccatg ttttttcatg
       61 aagatgagaa actaaagaag ctcaacacac ttgtaacgat tgaaaatcct tttcgggtga
      121 aacaatttag tcgcatgttt gatgagaata aaactgatcg aaacgatgca ttaagaatcg
      181 ctgatttctt gcgtattcaa cgattcacaa cttcccctat caaagaagag aaatacatgg
      241 ccttacagcg tttaacacgt actcgctatc aacttattaa gcaattgatt cgtacaaagc
      301 aacattttct tgaaaatcta acgtacaaat gtaatacgct tgctcgagaa atgcgagacg
      361 aatcgacgag tctttttagt gcaaccctca tttcactcat gacggaagat ttcacattgg
      421 atgaacttgc tgagcttcct ttagaagctt tctgtgattt acttcaagaa aaaggaaaag
      481 gccgtttcaa gcaaccagaa aaaatcgcta aagcaattca acgtgcaatt accatgagtt
      541 accgcctagg tgctcttgca caagaatcaa ttaatgttgt tttaagtgtt ctcgtacgag
      601 aaatacgagc acttgaaaaa aatatcaaag aattagataa agccatcgaa caagtaattg
      661 ttgtcattcc agaatatcaa tgcttaacca gtattccagg cgttggtaaa gtctatgctg
      721 ccggtttaat cgctgaaatt ggtcaaatcg aaagatttga agatcagaca aaattggcaa
      781 aatacgctgg attaagttgg aaagtgaacc aatctggaaa ctatcagtcg caaaatacac
      841 ctctgacaaa acaaggaaat cgatatttta gatactactt agttgaagct gccaactctg
      901 taaaaaatta tcttcctgaa tacaaagctt tttatcaaag taaatataag gaggttccaa
      961 agcatcaaca caaacgtgca ctcgtcctaa ccgcaagaaa atttacgcgt ctggtggata
     1021 cgctactacg taaacaccaa ctctatacgc caccaaggag tgtgatagag aaataacaat
     1081 ccgttattga cgcaaatcct tgaatcaacc cgaaaaaaat ttgattttga tcgggtctag
     1141 tttcgtgcgc ttcaaaacat attactcaat aaagaaaatc ttaaatttca tct
//
LOCUS       DACEEZ010000197         1158 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20313_2017
            SAMN12639966-rid9879223.denovo.209, whole genome shotgun sequence.
ACCESSION   DACEEZ010000197 DACEEZ010000000
VERSION     DACEEZ010000197.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639966
            Sequence Read Archive: SRR10023158
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1158)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1158)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741683.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741683.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:44:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,016
            CDSs (total)                      :: 2,962
            Genes (coding)                    :: 2,889
            CDSs (with protein)               :: 2,889
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 36 of 73
            Pseudo Genes (incomplete)         :: 29 of 73
            Pseudo Genes (internal stop)      :: 18 of 73
            Pseudo Genes (multiple problems)  :: 8 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1158
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639966-rid9879223.denovo.209"
                     /isolate="E20313_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            4..270
                     /locus_tag="IWO89_002907"
     CDS             4..270
                     /locus_tag="IWO89_002907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0457594.1"
                     /translation="MQSTGMKDKNGVDIFEGDIVLVSVRNGFDYLDNKVCIVKNSIDY
                     SGLVCATVDEDLEYQIFNTELFEEYTYEVTGNIYENSELLEEQR"
     gene            267..824
                     /locus_tag="IWO89_002908"
     CDS             267..824
                     /locus_tag="IWO89_002908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1642 domain-containing protein"
                     /protein_id="HAQ0457595.1"
                     /translation="MNKQEAIKKLESIKAIGNDAIAACYNESINSGITLMKKIDEPQK
                     PVIPQLVAGWLEKSTDPFTKAEKIAYLIKSKDGDSYYFCDWFVRDGIVTQEQGEELLA
                     WATRQSYETLLSLYNGYEVEKEPLWAIKNADGNYLTKCALWGKDGVNYSFECNPSHRL
                     LFTDKATADAAALLVNGTVEEVVER"
     gene            821..1117
                     /locus_tag="IWO89_002909"
     CDS             821..1117
                     /locus_tag="IWO89_002909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0457596.1"
                     /translation="MKLKDGFYASSHGIGGLMLDMPTKNPKTRKKSKFKVGDMVRCEA
                     EEFVYPFRGYVKKILSNSAIIRIENTMKCDRSTAKHKHYLAVARLVDMEVIKSK"
BASE COUNT          421 a          181 c          262 g          294 t
ORIGIN      
        1 ctcatgcaat ccacaggaat gaaagataag aatggtgtgg atatatttga aggggatata
       61 gtattagtca gcgtgcgaaa tggcttcgat tacttagata ataaagtctg tattgtcaaa
      121 aattcaatag attattccgg attagtttgt gccactgttg atgaagactt agagtatcaa
      181 atttttaaca cagagctgtt tgaagaatac acgtacgaag tcaccggaaa tatatacgag
      241 aatagcgagt tattggagga acagcgatga ataaacagga agcaatcaaa aagttagaaa
      301 gtattaaagc gataggaaat gatgcaatag ctgcttgcta taacgagagt ataaattcag
      361 gtattacgtt aatgaaaaaa atagacgaac cgcagaaacc agttattcca caactcgtgg
      421 ccggttggct tgagaaatct acggaccctt ttacaaaagc tgaaaaaata gcgtatttaa
      481 tcaaatctaa agatggtgat tcatattatt tctgtgattg gtttgtacga gatggcatag
      541 tgacgcaaga gcaaggagaa gaattactcg cttgggcaac aagacaatca tatgaaacac
      601 tattgagcct atacaacggc tacgaggttg agaaagagcc gttatgggca ataaagaatg
      661 ccgatggaaa ctatcttact aaatgtgctt tatggggaaa agatggagta aattatagtt
      721 ttgaatgcaa tccatctcat cgattgcttt tcactgataa agcaacagcg gatgctgcag
      781 cattgttggt gaatggaaca gtggaagagg tggtagaaag atgaaactaa aagacggatt
      841 ttacgctagc agtcacggta tcggcggttt aatgctagat atgccgacaa agaaccctaa
      901 aacacgcaag aaatcaaaat tcaaagtcgg tgacatggtc cgctgtgaag cagaagagtt
      961 cgtttatccg ttcagaggat acgttaaaaa gatactgtca aactcagcaa tcattcgtat
     1021 tgaaaataca atgaagtgtg atcgttcgac tgcaaaacac aagcattatt tagctgtagc
     1081 gagattagtt gacatggaag taatcaagag caaataaaaa aagccggatc gctccgactg
     1141 attcaataaa tccaacac
//
LOCUS       DACEEZ010000198         1147 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20313_2017
            SAMN12639966-rid9879223.denovo.210, whole genome shotgun sequence.
ACCESSION   DACEEZ010000198 DACEEZ010000000
VERSION     DACEEZ010000198.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639966
            Sequence Read Archive: SRR10023158
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1147)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1147)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741683.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741683.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:44:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,016
            CDSs (total)                      :: 2,962
            Genes (coding)                    :: 2,889
            CDSs (with protein)               :: 2,889
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 36 of 73
            Pseudo Genes (incomplete)         :: 29 of 73
            Pseudo Genes (internal stop)      :: 18 of 73
            Pseudo Genes (multiple problems)  :: 8 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1147
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639966-rid9879223.denovo.210"
                     /isolate="E20313_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(<1..153)
                     /locus_tag="IWO89_002910"
     CDS             complement(<1..153)
                     /locus_tag="IWO89_002910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS6 family transposase"
                     /protein_id="HAQ0457597.1"
                     /translation="MNHFKGKQFKKDVIIVAVGYYLRYNLSYREVQELLYDRGINVCH
                     TTIYRWV"
     gene            complement(475..696)
                     /locus_tag="IWO89_002911"
     CDS             complement(475..696)
                     /locus_tag="IWO89_002911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0457598.1"
                     /translation="MEEEKIFEKRWELASVEQRARYHNLMSSYRNIDWTYKEKKYLLW
                     LCQLDVNTFETFEVILDKIKNSNEKRADL"
     gene            complement(794..1039)
                     /locus_tag="IWO89_002912"
     CDS             complement(794..1039)
                     /locus_tag="IWO89_002912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008271463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0457599.1"
                     /translation="MDFPEVVKEVQEIGATISDSRSSPMNYQDAHGIKIIDQERNVRK
                     YTTINECVINENLCRAPITKHIKNRSKAKDGRTFTTF"
BASE COUNT          347 a          224 c          143 g          433 t
ORIGIN      
        1 tacccaacga taaatcgtag tatgacaaac atttattcca cgatcatata acaattcctg
       61 aacttcacga tagcttagat tgtaacgcag gtagtaacca acagcgacaa taatgacgtc
      121 ttttttgaat tgtttgcctt taaaatgatt cattactctg tcctctctgt cttttttctc
      181 aattttacac taaaatagat tttttggaaa actttgcaac agaaccgttt ttactatata
      241 cttcttccca gaacttgtcc gtctccttca aattatctct acctctcgag ttcattgggt
      301 ggtttgccca tttattaaac atggaatcac ctttctcata ctcatatatt tatgtcctat
      361 ttcaattata tttatatatg cttaaataaa tttgtatcct gaaatttatt gtaatatgaa
      421 gctgtatttt acatatagta accaaagagt taataaaata aaaagagcga accattatag
      481 atcagctctt ttttcattgc tgtttttaat cttatctaga atcacttcaa atgtttcaaa
      541 ggtatttaca tctaactgac ataaccaaag caagtacttc ttttctttgt aagtccaatc
      601 aatattacgg taagaggaca ttagattatg gtaccttgct ctttgttcaa ctgaagccaa
      661 ttcccagcgt ttttcaaata ttttttcttc ttccattatt gatccctcgc aagacgctac
      721 ctcaaactta aataatgggc agcgaaagtc taattcctta gatctaagta tagcaaacaa
      781 atatgaaaaa aacttagaag gtagtaaaag ttcttccgtc ttttgctttg cttctattct
      841 taatgtgttt cgttatcggt gccctacaaa gattttcatt aattacacac tcatttattg
      901 ttgtgtattt tctgacattt ctctcttgat caattatttt aattccatgg gcatcctgat
      961 agttcatagg agaggagcgg ctatcagaaa tagttgcacc tatctcttga acttctttta
     1021 caacttctgg aaaatccatc catcctgctt cagctgtcaa aagtaataat ctataatatt
     1081 tatctttcct tttcaatcta agaaatcgtc accccattct ttttcttcta ttataaacta
     1141 tttaact
//
LOCUS       DACEEZ010000199         1143 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20313_2017
            SAMN12639966-rid9879223.denovo.211, whole genome shotgun sequence.
ACCESSION   DACEEZ010000199 DACEEZ010000000
VERSION     DACEEZ010000199.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639966
            Sequence Read Archive: SRR10023158
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1143)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1143)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741683.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741683.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:44:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,016
            CDSs (total)                      :: 2,962
            Genes (coding)                    :: 2,889
            CDSs (with protein)               :: 2,889
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 36 of 73
            Pseudo Genes (incomplete)         :: 29 of 73
            Pseudo Genes (internal stop)      :: 18 of 73
            Pseudo Genes (multiple problems)  :: 8 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1143
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639966-rid9879223.denovo.211"
                     /isolate="E20313_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(18..923)
                     /gene="xerC"
                     /locus_tag="IWO89_002913"
     CDS             complement(18..923)
                     /gene="xerC"
                     /locus_tag="IWO89_002913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerC"
                     /protein_id="HAQ0457600.1"
                     /translation="MTEATWEQKFLRYLIVERGYSEKTKEAYEEDILHFKHFLEESGD
                     ADLLKAEYFTVRVYLSALADHRPSYSRNSISRKIASLRSFYQYLLKEEVIKENPFSYV
                     HLKKKNLRLPRFFYENEMQALFDSVAGDSPLDLRNRALLEVLYGSGIRLSECSSLSVS
                     DIDFDSEVMLIHGKGNKERYAPLGSFAQDALQEYFREGRQVLMTKYHEEHDIVFVNHH
                     GKPITPTGIEYVLNQMIKKSSLDSDIHPHMLRHTFATHLLNNGADLRTVQELLGHANL
                     STTQIYAHVTKESLQKNYRSFHPRA"
BASE COUNT          338 a          232 c          194 g          379 t
ORIGIN      
        1 tgttcttttc cttattttta cgctcttgga tggaaagaac gatagttttt ttgtaaactt
       61 tcttttgtta catgggcata gatctgggta gtcgataaat tggcatgtcc tagcaattct
      121 tggactgtcc gcaaatctgc cccattattt aataaatgag tagcaaatgt atggcgcagc
      181 atgtgaggat ggatatcact atccaaactg ctttttttga tcatttgatt caagacatat
      241 tcaattccag taggcgtgat tggttttcca tgatgattca caaaaacgat atcatgctct
      301 tcgtgatatt ttgtcatcaa tacctggcgg ccttccctga aatattcttg cagagcatct
      361 tgagcaaaag aacctagagg agcatatctc tctttgttcc ctttaccatg gatcaacatg
      421 acctctgaat caaaatcaat gtctgatacc gacaaactgc tacactcact taaacggatg
      481 cctgacccgt aaaggacttc cagtaaagcg cggttcctca aatccaaagg actatctcct
      541 gctacgctgt caaaaagtgc ttgcatttca ttttcataaa aaaaacgtgg cagtctcaaa
      601 tttttctttt tgagatgaac gtaagaaaat ggattttctt ttatcacttc ttctttgaga
      661 agatactgat aaaacgaacg taggctggct atcttgcgac tgatagaatt tctgctatag
      721 ctaggacgat gatcagctaa tgcactcaaa tagacacgaa ccgtaaaata ttccgccttc
      781 agcagatcag cgtctccaga ttcctccaaa aaatgtttga aatggagaat gtcttcttca
      841 tatgcttctt tcgttttttc tgaatatcct ctttcgacaa tcaaataacg caaaaatttt
      901 tgttcccacg tggcttctgt cattttctca ccttcctttt gtcccagaga ataatttagc
      961 acagcgttta taaaaagaca aataaattgt cagaatttaa ccaatatttc taagtttgtt
     1021 cacaattggc gcaattcctc acattttgtt tgagaaattc ttaagaaaaa cgcaagaaaa
     1081 agggctctat aatttatggg actgatgaat cagaattgat tcttcagccc cattttcatt
     1141 tta
//
LOCUS       DACEEZ010000200         1061 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20313_2017
            SAMN12639966-rid9879223.denovo.212, whole genome shotgun sequence.
ACCESSION   DACEEZ010000200 DACEEZ010000000
VERSION     DACEEZ010000200.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639966
            Sequence Read Archive: SRR10023158
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1061)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1061)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741683.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741683.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:44:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,016
            CDSs (total)                      :: 2,962
            Genes (coding)                    :: 2,889
            CDSs (with protein)               :: 2,889
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 36 of 73
            Pseudo Genes (incomplete)         :: 29 of 73
            Pseudo Genes (internal stop)      :: 18 of 73
            Pseudo Genes (multiple problems)  :: 8 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1061
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639966-rid9879223.denovo.212"
                     /isolate="E20313_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(36..992)
                     /locus_tag="IWO89_002914"
     CDS             complement(36..992)
                     /locus_tag="IWO89_002914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0457601.1"
                     /translation="MELYTLPIIIKTEKMLKDLKQYYPLSAYEKKKLFHFLAINLLAE
                     QADSLDIASNQSLFFSDIGFYHLKGVLGEKSVIFINKTLSWLYLHDKLDQKYLRKNED
                     ETIEWLNERFIESFENQFNLLREETSQLIRRELTKIHFKLLHYTINKADEFPLDVSFF
                     RENYPAFYCYLIEYINDLTSKRKELLQDKFFLFFSYLLLLINHIPIQLISEPVKIIID
                     FSYGAAYNQFIKKNLSLYINLNTEVIEAGKPEFPDVIITNRNNLYEESASQVVVWLDP
                     PRAVDWGNLTKILLTIQEEKYKDSKIDKQIDDFPEEIIDRIE"
BASE COUNT          346 a          177 c          160 g          378 t
ORIGIN      
        1 tagaaaaagc agtcggactt tctaaaagaa gttcactatt cgatcctgtc gatgatttct
       61 tcaggaaaat cgtctatttg cttgtcaatt ttgctgtctt tgtatttctc ttcttggata
      121 gtcaacaaaa tctttgtaag attcccccag tctactgctc ttggcggatc gagccaaacc
      181 actacttgag atgcactttc ttcatataga ttattcctat tcgtgattat aacatccggg
      241 aactcaggct ttcctgcctc tatcacttcc gtgttcagat taatgtacaa gcttaaattt
      301 ttcttgataa attgattata ggcagcacca taagaaaaat cgatgatgat ctttactggt
      361 tcagaaatca gttgaatggg tatgtgattg atcaataata ataaataact gaaaaaaaga
      421 aaaaatttat cttgaagcaa ctctttgcgt tttgacgtta gatcatttat atactctatc
      481 aaatagcaat aaaatgctgg atagttttct ctaaaaaaag aaacatcaag tggaaattca
      541 tcagctttat taatagtgta atgcaaaagt ttaaaatgta ttttggttaa ctctcttcgg
      601 atcaattggc tcgtctcttc tcgcaacaga ttaaactgat tttcaaaact ttcaatgaat
      661 cgttcattca accattcgat tgtttcatct tcattttttc tcaagtattt ttgatccaat
      721 ttatcatgaa gataaagcca agagagtgtc ttattgataa aaataacgga tttttctcct
      781 aatactcctt ttaagtggta aaatcctata tccgaaaaaa atagcgactg attgctagca
      841 atatctaaag aatcagcttg ttcagccaac aggttgattg ctaaaaagtg gaataatttc
      901 tttttttcat aagcagacag aggataatac tgttttaaat cttttaacat tttttctgtt
      961 tttataatta taggtagcgt atacaattcc acaaaactta tctctgttga gttaaaaaga
     1021 ttatataaaa aaagccgaat gtttttagga ttccctgtca a
//
LOCUS       DACEEZ010000201         1051 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20313_2017
            SAMN12639966-rid9879223.denovo.213, whole genome shotgun sequence.
ACCESSION   DACEEZ010000201 DACEEZ010000000
VERSION     DACEEZ010000201.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639966
            Sequence Read Archive: SRR10023158
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1051)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1051)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741683.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741683.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:44:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,016
            CDSs (total)                      :: 2,962
            Genes (coding)                    :: 2,889
            CDSs (with protein)               :: 2,889
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 36 of 73
            Pseudo Genes (incomplete)         :: 29 of 73
            Pseudo Genes (internal stop)      :: 18 of 73
            Pseudo Genes (multiple problems)  :: 8 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1051
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639966-rid9879223.denovo.213"
                     /isolate="E20313_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            6..980
                     /locus_tag="IWO89_002915"
     CDS             6..980
                     /locus_tag="IWO89_002915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choloylglycine hydrolase family protein"
                     /protein_id="HAQ0457602.1"
                     /translation="MCTSITYVTSDHYFGRNFDYEISYNEVVTVTPRNYKLNFRKVND
                     LDTHYAMIGIAAGIADYPLYYDATNEKGLSMAGLNFSGYADYKEIQEGKDNVSPFEFI
                     PWILGQCSTVGEAKKLLKNINLANINYSDELPLSPLHWLLADKEKSIVIESMKDGLHI
                     YDNPVGVLTNNPSFDYQLFNLNNYRVLSSETPKNNFSNQISLNAYSRGMGGIGLPGDL
                     SSVSRFVKATFTKLNSVSGDSESESISQFFHILGSVEQQKGLCDVGDGKYEYTIYSSC
                     CNVDKGIYYYRTYEDSQITAIDMNKEDLDSHKLISYPIIEKQQIKYIN"
BASE COUNT          377 a          132 c          182 g          360 t
ORIGIN      
        1 aaactatgtg tacgtctatt acttatgtaa caagtgatca ttattttgga aggaattttg
       61 attatgaaat atcttacaat gaagtagtta ctgttactcc aagaaattat aagttgaatt
      121 ttcgaaaggt aaatgatttg gatactcatt atgcaatgat tggtattgcc gctggtatag
      181 ctgactaccc tctttattac gatgcgacaa atgaaaaagg attgagtatg gctgggctaa
      241 atttttctgg gtatgctgat tataaagaaa tacaagaagg gaaagacaat gtatctcctt
      301 ttgaatttat tccttggatt ttaggacaat gctcaacagt aggagaagct aaaaaattgt
      361 taaaaaatat caatttagca aatataaatt atagtgacga acttccttta tcccctttac
      421 attggctatt agctgataaa gaaaaatcaa ttgtcattga aagtatgaaa gatggacttc
      481 atatatatga taaccctgtg ggcgttctta ccaataatcc ttcatttgac tatcaattat
      541 ttaatttaaa caattatcgt gtcttatcga gtgaaactcc taaaaataat ttttcaaatc
      601 aaataagttt gaatgcctat agccgcggta tgggagggat aggcttgcct ggagatttat
      661 cctcagtatc tcgttttgtt aaagcgactt ttacgaagct gaattctgta tctggagatt
      721 cagagtcaga aagtattagt caatttttcc atatcttagg ttcagtagaa caacaaaaag
      781 gtttgtgtga tgtaggtgat ggaaaatatg aatatacaat ttattcttct tgttgcaatg
      841 ttgacaaagg aatctattat tatcgaacat atgaagacag tcaaattact gcaattgata
      901 tgaataaaga agacttagat agtcataagt taattagtta tccaattata gaaaaacaac
      961 aaattaaata tataaattag ttaatgtgtt gtgattgatt atttaatata gatataatac
     1021 aaaaaggcaa agatattttc aaaacatttt t
//
LOCUS       DACEEZ010000202          994 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20313_2017
            SAMN12639966-rid9879223.denovo.214, whole genome shotgun sequence.
ACCESSION   DACEEZ010000202 DACEEZ010000000
VERSION     DACEEZ010000202.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639966
            Sequence Read Archive: SRR10023158
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 994)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 994)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741683.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741683.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:44:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,016
            CDSs (total)                      :: 2,962
            Genes (coding)                    :: 2,889
            CDSs (with protein)               :: 2,889
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 36 of 73
            Pseudo Genes (incomplete)         :: 29 of 73
            Pseudo Genes (internal stop)      :: 18 of 73
            Pseudo Genes (multiple problems)  :: 8 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..994
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639966-rid9879223.denovo.214"
                     /isolate="E20313_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(25..912)
                     /locus_tag="IWO89_002916"
     CDS             complement(25..912)
                     /locus_tag="IWO89_002916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HAQ0457603.1"
                     /translation="MQELKTRSARHHRPKRKMVINKKAIAPLIGTSLLVGPMMVTAVP
                     QHVHADEQGSQTVADPQAFIDQIGWSASEVAASNDLYASVMIAQALLESAYGTSGLAS
                     APNYNLFGVKGSYNGQVVYMATKEYVNGQWVTMNEPFRKYSSYWESFQDHANVLRSTS
                     FSTGDYHYAGVWKSNTTSFYDATAYLTGRYATDPNYGAKLNSLINTYNLTRFDTPSTG
                     SATTTTSTTTTTTTTTSTSSSSTTDVNTNNGQTYTVVSGDTLWDIAQKFGMSVDDLMA
                     KNGMSMSSYSLLVGQELNV"
BASE COUNT          288 a          207 c          179 g          320 t
ORIGIN      
        1 aatttctttg cctttttatc taaatcaaac gtttagttct tgaccaacta ataaagaata
       61 actggacata gacatgccat ttttcgccat tagatcatca acagacatgc caaatttttg
      121 tgcaatatcc catagcgtat cacctgacac gactgtatat gtctgaccat tatttgtatt
      181 tacatctgtt gttgaagatg atgacgttga agtcgtcgta gtagtcgttg ttgttgtact
      241 tgtggttgtt gtagccgaac ctgttgatgg cgtatcaaaa cgggttaaat tataagtatt
      301 aatcaatgag ttcaatttgg ccccataatt tggatcggta gcataacgac ctgtcaagta
      361 cgctgttgca tcataaaaac ttgttgtatt gcttttccac acgccagcat aatgataatc
      421 gcccgtggaa aaactggttg aacgaagaac attcgcatgg tcttggaagg attcccaata
      481 agaagaatat tttcggaatg gctcattcat cgttacccat tgtccgttta catattcttt
      541 ggttgccata taaacgacct gtccgttata gcttcctttc acaccgaata gattataatt
      601 tggcgcacta gctaatccgg aagtgccata tgcactttcc aacaacgctt gagcgatcat
      661 gactgaagca tacaagtcat tacttgcagc aacttctgac gcagaccagc cgatttgatc
      721 gataaacgct tgaggatcag cgactgtttg agatccttgt tcatctgcat gaacatgttg
      781 cggtaccgct gtaaccatca tcggcccaac taaaagactc gttccaatta aaggagcaat
      841 cgctttttta ttgatcacca tttttctttt aggtctatgg tgccgggcac tgcgggtctt
      901 cagctcttgc attcaaattc ctcctcaaaa taaaaaaact taataaaaag ttctcttctt
      961 aaaacaaact taattaatta aattatactt ttaa
//
LOCUS       DACEEZ010000203          994 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20313_2017
            SAMN12639966-rid9879223.denovo.215, whole genome shotgun sequence.
ACCESSION   DACEEZ010000203 DACEEZ010000000
VERSION     DACEEZ010000203.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639966
            Sequence Read Archive: SRR10023158
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 994)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 994)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741683.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741683.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:44:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,016
            CDSs (total)                      :: 2,962
            Genes (coding)                    :: 2,889
            CDSs (with protein)               :: 2,889
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 36 of 73
            Pseudo Genes (incomplete)         :: 29 of 73
            Pseudo Genes (internal stop)      :: 18 of 73
            Pseudo Genes (multiple problems)  :: 8 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..994
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639966-rid9879223.denovo.215"
                     /isolate="E20313_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(<1..>994)
                     /locus_tag="IWO89_002917"
     rRNA            complement(<1..>994)
                     /locus_tag="IWO89_002917"
                     /product="16S ribosomal RNA"
BASE COUNT          209 a          307 c          225 g          253 t
ORIGIN      
        1 agcacgtgtg tagcccaggt cataaggggc atgatgattt gacgtcatcc ccaccttcct
       61 ccggtttgtc accggcagtc ttgctagagt gcccaactga atgatggcaa ctaacaataa
      121 gggttgcgct cgttgcggga cttaacccaa catctcacga cacgagctga cgacaaccat
      181 gcaccacctg tcactttgcc cccgaagggg aagctctatc tctagagtgg tcaaaggatg
      241 tcaagacctg gtaaggttct tcgcgttgct tcgaattaaa ccacatgctc caccgcttgt
      301 gcgggccccc gtcaattcct ttgagtttca accttgcggt cgtactcccc aggcggagtg
      361 cttaatgcgt tagctgcagc actgaagggc ggaaaccctc caacacttag cactcatcgt
      421 ttacggcgtg gactaccagg gtatctaatc ctgtttgctc cccacgcttt cgagcctcag
      481 cgtcagttac agaccagaga gccgccttcg ccactggtgt tcctccatat atctacgcat
      541 ttcaccgcta cacatggaat tccactctcc tcttctgcac tcaagtctcc cagtttccaa
      601 tgaccctccc cggttgagcc gggggctttc acatcagact taagaaaccg cctgcgctcg
      661 ctttacgccc aataaatccg gacaacgctt gccacctacg tattaccgcg gctgctggca
      721 cgtagttagc cgtggctttc tggttagata ccgtcaaggg atgaacagtt actctcatcc
      781 ttgttcttct ctaacaacag agttttacga tccgaaaacc ttcttcactc acgcggcgtt
      841 gctcggtcag actttcgtcc attgccgaag attccctact gctgcctccc gtaggagttt
      901 gggccgtgtc tcagtcccaa tgtggccgat caccctctca ggtcggctat gcatcgtggc
      961 cttggtgagc cgttacctca ccaactagct aatg
//
LOCUS       DACEEZ010000204          988 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20313_2017
            SAMN12639966-rid9879223.denovo.216, whole genome shotgun sequence.
ACCESSION   DACEEZ010000204 DACEEZ010000000
VERSION     DACEEZ010000204.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639966
            Sequence Read Archive: SRR10023158
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 988)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 988)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741683.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741683.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:44:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,016
            CDSs (total)                      :: 2,962
            Genes (coding)                    :: 2,889
            CDSs (with protein)               :: 2,889
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 36 of 73
            Pseudo Genes (incomplete)         :: 29 of 73
            Pseudo Genes (internal stop)      :: 18 of 73
            Pseudo Genes (multiple problems)  :: 8 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..988
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639966-rid9879223.denovo.216"
                     /isolate="E20313_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            <1..704
                     /locus_tag="IWO89_002918"
     CDS             <1..704
                     /locus_tag="IWO89_002918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="HAQ0457604.1"
                     /translation="YSVIFMDATHIPVKRQTVSKEAVYITIGIRLDGTKEVLGFTIAP
                     TESAYIWKEVLQDLRKRGLEEVLLVVTDGLSCIEESIHSVYPNAQFQQCCVHVSRNIA
                     HKVRVRGRKEICEDFKLVYQANSKEEALDHIDFMTRKWKKQYPRVVNLLLNPALLTFY
                     NFPHAIRRTIYSTNLIEGFNKQLKRYTRRKEQFPNEESLERFFISQFNQYNQKFLGRI
                     HKGFKEIQDTLESMI"
BASE COUNT          335 a          161 c          163 g          329 t
ORIGIN      
        1 attattccgt tatatttatg gatgcgacgc atatccctgt gaagcgacag actgtttcga
       61 aggaagcagt gtatattacg attggtattc gtttggatgg aaccaaagaa gttcttgggt
      121 ttactattgc cccaactgag tctgcttata tttggaaaga agttcttcaa gatcttagaa
      181 aacgtgggtt agaagaagtt ttattagtag tgacagacgg attaagctgt attgaagaaa
      241 gtatccatag tgtgtatccg aatgcccaat ttcaacaatg ttgtgtgcat gtatctagaa
      301 atatcgctca taaagttcgt gttcgaggtc gaaaagaaat ttgtgaggat ttcaaattgg
      361 tttaccaagc gaattcaaaa gaagaggcat tggatcacat cgactttatg actaggaaat
      421 ggaaaaagca gtatccaaga gtcgtcaatt tactcttgaa tcctgcccta ttaacctttt
      481 ataatttccc tcacgccatc agacgaacaa tttattcgac gaacctgatt gaaggcttta
      541 ataagcagct aaaacgatat actcgaagaa aagaacaatt ccctaatgaa gaatctctag
      601 agagattctt catttctcaa tttaatcaat ataaccaaaa atttttaggt agaattcaca
      661 agggatttaa agaaattcag gatacattag agtcgatgat ttaactgtaa ttaacagaat
      721 ggattttcca tttacatata attcttgacg ctaccataat ctaaataacc atcatattca
      781 tctactactt atataattga gttttattaa actttttaag tttctattct atcaaatatt
      841 taaacaatct ctgcatccga aaagactacc tcgtatagtt ttactaacaa tatttttatt
      901 agcgtgttca aatcctgatt tcttgtatca attattttct gttcactttc aataagaaaa
      961 aagtctttac ttccatggat aataataa
//
LOCUS       DACEEZ010000205          968 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20313_2017
            SAMN12639966-rid9879223.denovo.217, whole genome shotgun sequence.
ACCESSION   DACEEZ010000205 DACEEZ010000000
VERSION     DACEEZ010000205.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639966
            Sequence Read Archive: SRR10023158
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 968)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 968)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741683.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741683.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:44:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,016
            CDSs (total)                      :: 2,962
            Genes (coding)                    :: 2,889
            CDSs (with protein)               :: 2,889
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 36 of 73
            Pseudo Genes (incomplete)         :: 29 of 73
            Pseudo Genes (internal stop)      :: 18 of 73
            Pseudo Genes (multiple problems)  :: 8 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..968
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639966-rid9879223.denovo.217"
                     /isolate="E20313_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(12..83)
                     /locus_tag="IWO89_002919"
     tRNA            complement(12..83)
                     /locus_tag="IWO89_002919"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(48..50),aa:Glu,seq:ttc)
     gene            complement(95..168)
                     /locus_tag="IWO89_002920"
     tRNA            complement(95..168)
                     /locus_tag="IWO89_002920"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(132..134),aa:Ile,seq:gat)
     gene            complement(181..251)
                     /locus_tag="IWO89_002921"
     tRNA            complement(181..251)
                     /locus_tag="IWO89_002921"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(217..219),aa:Gly,seq:tcc)
     gene            complement(256..328)
                     /locus_tag="IWO89_002922"
     tRNA            complement(256..328)
                     /locus_tag="IWO89_002922"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(293..295),aa:Phe,seq:gaa)
     gene            complement(332..404)
                     /locus_tag="IWO89_002923"
     tRNA            complement(332..404)
                     /locus_tag="IWO89_002923"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(369..371),aa:Asp,seq:gtc)
     gene            complement(423..496)
                     /locus_tag="IWO89_002924"
     tRNA            complement(423..496)
                     /locus_tag="IWO89_002924"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(460..462),aa:Met,seq:cat)
     gene            complement(509..598)
                     /locus_tag="IWO89_002925"
     tRNA            complement(509..598)
                     /locus_tag="IWO89_002925"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(560..562),aa:Ser,seq:tga)
     gene            complement(621..692)
                     /locus_tag="IWO89_002926"
     tRNA            complement(621..692)
                     /locus_tag="IWO89_002926"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(657..659),aa:Glu,seq:ttc)
     gene            complement(714..801)
                     /locus_tag="IWO89_002927"
     tRNA            complement(714..801)
                     /locus_tag="IWO89_002927"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(765..767),aa:Ser,seq:gct)
     gene            complement(826..899)
                     /locus_tag="IWO89_002928"
     tRNA            complement(826..899)
                     /locus_tag="IWO89_002928"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(863..865),aa:Ile,seq:gat)
BASE COUNT          269 a          262 c          231 g          206 t
ORIGIN      
        1 agtatcttcc atgacccgta cgggattcga acccgtgtta ccgccgtgaa agggcggtgt
       61 cttaaccgct tgaccaacgg gccaataaaa taaatgggcc taaatggact cgaaccatcg
      121 acctcacgct tatcaggcgt gcgctctaac cagctgagct ataggcccat aaaaactaaa
      181 agcgggtgac gagaatcgaa ctcgcgacaa cagcttggaa ggctgtggtt ttaccactaa
      241 actacacccg cataatggcg cgggacagaa tcgaactgcc gacacatgga gcttcaatcc
      301 attgctctac caactgagct accgagccaa acggttccga cgggaatcga acccgcgatc
      361 tcctgcgtga caggcaggca tgttaacccc tacaccacgg aaccaatgtt tttagttttt
      421 aattgcggga gcaggatttg aacctacgac cttcgggtta tgagcccgac gagctaccag
      481 actgctccat cccgcgataa tatgaaaaag gaggataagg gattcgaacc cttgcacggt
      541 tttacccgcc tgacggtttt caagaccgtt cccttcagcc ggacttgggt aatcctccat
      601 gtaaaactat attaaattaa tgacccgtac gggattcgaa cccgtgttac cgccgtgaaa
      661 gggcggtgtc ttaaccgctt gaccaacggg ccaggtaaaa caaaatattg aaacggagaa
      721 ggagggattt gaaccctcgc gccggtttcc cgacctacac ccttagcagg ggcgcctctt
      781 cagccacttg agtacttccc caaaaccaat aagattttgt actaatgggc ctaaatggac
      841 tcgaaccatc gacctcacgc ttatcaggcg tgcgctctaa ccagctgagc tataggccca
      901 taaaaaacta aaagcgggtg acgagaatcg aactcgcgac aacagcttgg aaggctgtgg
      961 ttttacca
//
LOCUS       DACEEZ010000206          926 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20313_2017
            SAMN12639966-rid9879223.denovo.218, whole genome shotgun sequence.
ACCESSION   DACEEZ010000206 DACEEZ010000000
VERSION     DACEEZ010000206.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639966
            Sequence Read Archive: SRR10023158
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 926)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 926)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741683.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741683.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:44:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,016
            CDSs (total)                      :: 2,962
            Genes (coding)                    :: 2,889
            CDSs (with protein)               :: 2,889
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 36 of 73
            Pseudo Genes (incomplete)         :: 29 of 73
            Pseudo Genes (internal stop)      :: 18 of 73
            Pseudo Genes (multiple problems)  :: 8 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..926
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639966-rid9879223.denovo.218"
                     /isolate="E20313_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(151..>926)
                     /gene="menA"
                     /locus_tag="IWO89_002929"
     CDS             complement(151..>926)
                     /gene="menA"
                     /locus_tag="IWO89_002929"
                     /EC_number="2.5.1.74"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoate
                     polyprenyltransferase"
                     /protein_id="HAQ0457605.1"
                     /translation="YMDYEKAHSEVYKYEENVIGRRNLSPVLIRNMILAMIAFVFVIG
                     VILTINTGWLLLLMGFICCFIGVFYTFGPIPLSRMPLGEIFSGVTMGLGIFAMVVYVN
                     TFGSDVYDLTVNFSSGQFDLVGNLWAIAAIVLASLPLVFTIANIMLANNLRDLDRDIE
                     NHRYTLVFYIGRPAGTVLFQLLMYACYFVILMGLLSGIYEWPILVTFLTLPIVKKHLD
                     QFKQTLPQPISFSYSIKNMVLFNASYVLGLLLTIIMQAI"
BASE COUNT          328 a          193 c          160 g          245 t
ORIGIN      
        1 cgactttttc acaacctcct gataaacggt gttcaaaagc ttgcatctgc ggtaataagc
       61 ccaaccaaac gaaaaatata aggagctatt tttatttggc tgttcttatt acttgatgca
      121 gggcagtttt ttcacaacct cttcatgctg ttaaatggct tgcataatga ttgttagtaa
      181 tagccccaat acataactgg cattgaataa gaccatattt ttgatggaat agctgaaact
      241 gataggctgg ggtaacgttt gcttgaattg atccaaatgc ttcttcacaa ttggcaaggt
      301 caagaaggtg accaagatcg gccattcata gataccgctc aacaacccca ttaaaatcac
      361 gaaatagcaa gcatacatca atagctggaa taagactgtt cccgcaggac gtccgatata
      421 aaaaactagt gtatagcgat gattttcaat atcacggtca agatcccgca aattattagc
      481 caacatgata ttggcaatcg taaacaccaa gggtaaggaa gctaatacga ttgctgctat
      541 agcccaaaga tttcctacca aatcaaattg tccagatgaa aagttgactg tcagatcata
      601 gacatctgaa ccgaatgtat tgacataaac gaccatggca aaaattccaa ggcccatagt
      661 caccccactg aaaatttccc ctaaaggcat tcgagataaa ggaatcggtc cgaatgtata
      721 gaatactcca ataaaacaac agataaatcc catcaaaagt aaaagccaac ccgtattgat
      781 cgttagaata acgccgataa caaaaacaaa tgcaatcata gctaaaatca tgtttcgaat
      841 caatacagga gatagatttc tgcggccaat gacattttct tcatacttat atacttcaga
      901 atgagccttt tcataatcca tgtaat
//
LOCUS       DACEEZ010000207          861 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20313_2017
            SAMN12639966-rid9879223.denovo.219, whole genome shotgun sequence.
ACCESSION   DACEEZ010000207 DACEEZ010000000
VERSION     DACEEZ010000207.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639966
            Sequence Read Archive: SRR10023158
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 861)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 861)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741683.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741683.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:44:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,016
            CDSs (total)                      :: 2,962
            Genes (coding)                    :: 2,889
            CDSs (with protein)               :: 2,889
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 36 of 73
            Pseudo Genes (incomplete)         :: 29 of 73
            Pseudo Genes (internal stop)      :: 18 of 73
            Pseudo Genes (multiple problems)  :: 8 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..861
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639966-rid9879223.denovo.219"
                     /isolate="E20313_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            40..423
                     /locus_tag="IWO89_002930"
     CDS             40..423
                     /locus_tag="IWO89_002930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0457606.1"
                     /translation="MLVSVPYTDQPPLEGIRADGEPLTIADQMFDPKLNQWIILANAL
                     DHNDLNNLKAMYESLENENGDLKQINAKLMLSDVAIKQENTALKEKADSLAQINSKMM
                     LASLQNSKDISEIKEQLNPASKGGE"
     gene            425..562
                     /locus_tag="IWO89_002931"
     CDS             425..562
                     /locus_tag="IWO89_002931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XkdX family protein"
                     /protein_id="HAQ0457607.1"
                     /translation="MFSFSDVKMMYDWGCFTNEQVMVFVPLCITEEKADKIISKEESA
                     S"
     gene            600..>861
                     /locus_tag="IWO89_002932"
     CDS             600..>861
                     /locus_tag="IWO89_002932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0457608.1"
                     /translation="MINLGEWGAIAGSITAIVSLILLVIKPITASFSKITETLSKVSH
                     NLDLLTKDLESSKSDRLMIHEELKKHDERLDTHAEKLVEHTQQ"
BASE COUNT          314 a          136 c          177 g          234 t
ORIGIN      
        1 cgtactggcg gagagtacgc aggaaaagta ttggacgaca tgcttgtatc cgttccttac
       61 acagatcagc caccgcttga aggaattcgt gctgatggcg aaccgttaac aatcgctgat
      121 caaatgtttg atcctaaatt gaatcaatgg attattttag cgaacgcact agatcacaac
      181 gatttaaaca atctcaaagc aatgtatgag tcgttagaaa atgagaacgg cgatttaaaa
      241 cagatcaatg ccaaactcat gctaagcgat gtagcaatta aacaggaaaa tactgcattg
      301 aaagaaaaag cggatagttt agcacaaatc aattcaaaaa tgatgcttgc ttcgttacaa
      361 aatagcaaag acatttcaga aattaaagag caactaaatc cagcttcaaa gggaggtgag
      421 tagtatgttt agttttagcg atgtgaaaat gatgtatgat tggggctgtt tcactaacga
      481 acaagtaatg gttttcgttc cgttgtgcat tactgaagaa aaagcagata aaatcattag
      541 caaagaagag agcgcatctt aattgatgtg ctttttattt tgattcaagg agttgtcaca
      601 tgattaattt aggggaatgg ggagcgatag caggatcaat aaccgctatc gtttctttga
      661 ttttattagt aataaaacca attactgcat ctttctcgaa gattactgag actctttcaa
      721 aagtaagtca caatttagat ttgctgacta aagatttaga atcgagcaaa tcagatcgat
      781 tgatgattca tgaagaacta aagaaacacg atgaaagatt agatacacat gcagaaaaat
      841 tggtagaaca cacgcaacaa a
//
LOCUS       DACEEZ010000208          861 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20313_2017
            SAMN12639966-rid9879223.denovo.220, whole genome shotgun sequence.
ACCESSION   DACEEZ010000208 DACEEZ010000000
VERSION     DACEEZ010000208.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639966
            Sequence Read Archive: SRR10023158
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 861)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 861)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741683.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741683.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:44:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,016
            CDSs (total)                      :: 2,962
            Genes (coding)                    :: 2,889
            CDSs (with protein)               :: 2,889
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 36 of 73
            Pseudo Genes (incomplete)         :: 29 of 73
            Pseudo Genes (internal stop)      :: 18 of 73
            Pseudo Genes (multiple problems)  :: 8 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..861
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639966-rid9879223.denovo.220"
                     /isolate="E20313_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            <1..836
                     /locus_tag="IWO89_002933"
     CDS             <1..836
                     /locus_tag="IWO89_002933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000997695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS256-like element ISEf1 family transposase"
                     /protein_id="HAQ0457609.1"
                     /translation="EIADLIEKMYGHHYTPQTMSNMTKVLTEEVNAFKSRALNDKYVA
                     IFMDATYIPLKRQTVSKEAIYIAIGIREDGTKEVLSYAIAPTESTYVWNELLQDINSR
                     GVQEVLLFITDGLKGMKDTIHQIYPKAKYQHCCIHVSRNIAHKVRVKDRKEICDDFKA
                     VYQANSKEEANTFLSGMIEKWKKNYPKVTQSLIENQDLLTFYDFPPSIRRTIYSTNLI
                     ESFNKQIKRYSRRKEQFQNEESLERFLVSIFDTYNQKFLNRSHKGFQQVTDTLVSMFT
                     E"
BASE COUNT          313 a          162 c          144 g          242 t
ORIGIN      
        1 ctgaaattgc tgatttgatc gaaaaaatgt acggtcatca ctatactcca caaaccatgt
       61 ccaacatgac taaagttctg actgaagaag taaatgcctt taaatccaga gccttaaatg
      121 ataagtatgt cgctattttt atggacgcta cttacattcc actaaaacgt caaaccgtat
      181 ccaaagaagc gatttatatt gccattggta tacgagaaga cggcactaaa gaagtactga
      241 gttatgcgat tgctccaact gaatcaacat acgtttggaa tgagctgcta caggatatta
      301 actccagagg agttcaagaa gtcttgcttt ttattacgga cggcttaaaa ggcatgaaag
      361 atactatcca tcaaatttat cctaaagcaa aatatcagca ttgttgtatc catgtatctc
      421 gtaacatcgc tcataaagta cgtgtcaaag accgaaaaga aatctgtgat gactttaagg
      481 ctgtttatca agctaactca aaagaagaag cgaatacctt cttatccggc atgattgaga
      541 aatggaagaa aaactatcct aaagtgacgc agtcactcat agaaaaccaa gacttattaa
      601 ctttttatga ttttccacct agcattcgta gaaccattta ctcaaccaat ctaatcgagt
      661 ctttcaataa gcaaattaaa agatacagcc gtagaaaaga gcagtttcaa aatgaagaat
      721 cactagaacg ctttctagtc agcatttttg atacatacaa tcaaaaattt ctaaacagaa
      781 gccataaagg ttttcaacag gtaaccgata cattagtttc aatgtttact gagtaactaa
      841 ttattttgca ggaggacaat t
//
LOCUS       DACEEZ010000209          846 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20313_2017
            SAMN12639966-rid9879223.denovo.221, whole genome shotgun sequence.
ACCESSION   DACEEZ010000209 DACEEZ010000000
VERSION     DACEEZ010000209.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639966
            Sequence Read Archive: SRR10023158
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 846)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 846)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741683.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741683.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:44:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,016
            CDSs (total)                      :: 2,962
            Genes (coding)                    :: 2,889
            CDSs (with protein)               :: 2,889
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 36 of 73
            Pseudo Genes (incomplete)         :: 29 of 73
            Pseudo Genes (internal stop)      :: 18 of 73
            Pseudo Genes (multiple problems)  :: 8 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..846
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639966-rid9879223.denovo.221"
                     /isolate="E20313_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            15..>846
                     /locus_tag="IWO89_002934"
     CDS             15..>846
                     /locus_tag="IWO89_002934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HAQ0457610.1"
                     /translation="MTYTHLTTDELVIIESYFKMNQSVAKTAHCLNRSRQTIHKVYLF
                     FKQGKSALEYYQQYKKNKSNCGRRPLVLPEEQSEYIQRKVVQGWTPDVIVGRAAFPIS
                     CSARTIYRMFKKGLFDSSDLPMKGKRKPNGHQERRGKQTFRRSIHEREKDYSQFSNEF
                     GHLEGDTIVGLKHKSAVITLVERLSKVIITLKPCGRQAIDIEKKLNQWFESVPKNLFK
                     SITFDCGKEFSNWKQISNVNDIAIYFADPGTPSQRGLNENSNGLLRRDGLLKSMDFNS
                     V"
BASE COUNT          290 a          147 c          164 g          245 t
ORIGIN      
        1 aggagtgagt acaaatgacc tatacccatc ttacaacgga tgaacttgta ataatagagt
       61 cttatttcaa aatgaatcaa tctgttgcta aaactgccca ttgcttgaat cgttcaagac
      121 aaacgatcca taaagtatat ctgttcttca agcaaggaaa atcagcctta gaatattatc
      181 aacagtataa gaaaaacaaa tcaaactgtg gtagacgtcc gcttgtttta cccgaggaac
      241 aatcagaata tattcaaaga aaggttgttc aaggatggac acccgatgtg attgttggtc
      301 gtgcagcgtt tcctattagt tgttctgctc gtaccattta tcgtatgttc aaaaaggggc
      361 tatttgattc ttctgactta ccgatgaaag gcaagcgtaa accgaatgga catcaagaaa
      421 gacgtggaaa acaaactttc cgccgctcta ttcatgaacg tgaaaaggat tatagccaat
      481 tctcaaatga gtttggtcac cttgaaggtg acactatcgt aggtctgaaa cataaaagtg
      541 ctgtaattac cttagttgaa cgattatcaa aagttatcat cacattgaaa ccgtgtggta
      601 gacaagcgat tgatattgaa aaaaaattaa atcaatggtt tgaatctgta ccgaaaaacc
      661 tattcaaatc catcactttt gattgtggaa aggaattttc aaattggaaa cagatcagta
      721 atgtcaatga tattgccatt tatttcgctg atccaggaac gccgtctcaa agaggcctaa
      781 acgagaattc taacggattg ttacgtagag atggtttatt gaaatctatg gatttcaatt
      841 cagtag
//
LOCUS       DACEEZ010000210          837 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20313_2017
            SAMN12639966-rid9879223.denovo.222, whole genome shotgun sequence.
ACCESSION   DACEEZ010000210 DACEEZ010000000
VERSION     DACEEZ010000210.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639966
            Sequence Read Archive: SRR10023158
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 837)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 837)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741683.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741683.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:44:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,016
            CDSs (total)                      :: 2,962
            Genes (coding)                    :: 2,889
            CDSs (with protein)               :: 2,889
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 36 of 73
            Pseudo Genes (incomplete)         :: 29 of 73
            Pseudo Genes (internal stop)      :: 18 of 73
            Pseudo Genes (multiple problems)  :: 8 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..837
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639966-rid9879223.denovo.222"
                     /isolate="E20313_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            1..837
                     /locus_tag="IWO89_002935"
                     /pseudo
     CDS             1..837
                     /locus_tag="IWO89_002935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611105.1"
                     /note="frameshifted; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3-like element ISEnfa3 family transposase"
BASE COUNT          363 a          116 c          142 g          216 t
ORIGIN      
        1 atggaaacaa atggtctttc tatccaagaa actgctaaaa aatttaatat cggttcaaat
       61 agaactctaa gtaaatggat agagcaatat gaagaaggcg gtgcttcttc acttgagagc
      121 caaaaaaggg gcagaaaaat tagtatgaat tccaagctaa acattcctaa aaaacttaaa
      181 gatgagtctc ttgaagaaga agttattcgt ttaagagcag agaacgcata tttaaaaaag
      241 ttagaaacct tgattcaaga acaagatttg agcaagaaga aatcaaggtt aaaacaatcc
      301 ttcagctcaa aaaagaattt aaattgaatc tattactttc aattgcacaa ttagcgaagt
      361 caacttatta ttactgggta aaaaaattag ataagccaga taaatatagc aagataaagc
      421 aagaaattac agcgattgta aaagaatcaa gaaactctta tggttatcgt agagtcactt
      481 tagcgttaaa gatgaaagga tatacaatca atcataaaac agttagaaaa ttaatgtccc
      541 aaatgggact tacctgtcaa atccgaataa agcgatataa atcttataaa ggaacagtag
      601 gaaaaattgc caagaatgtg ttaaaacgaa atttttcagt agatacaccc aataaaaaat
      661 gggtgacaga cgtcactgaa ttcaagataa aaggaagaaa aatctatcta tctcctatac
      721 ttgatttatt taacggagaa ataattagtt atagtatatc aactagtcca acatataaac
      781 tgattgaaga aatgcttcaa caagcaatta aaaaaaaggg aactgagggt tcactga
//
LOCUS       DACEEZ010000211          802 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20313_2017
            SAMN12639966-rid9879223.denovo.223, whole genome shotgun sequence.
ACCESSION   DACEEZ010000211 DACEEZ010000000
VERSION     DACEEZ010000211.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639966
            Sequence Read Archive: SRR10023158
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 802)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 802)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741683.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741683.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:44:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,016
            CDSs (total)                      :: 2,962
            Genes (coding)                    :: 2,889
            CDSs (with protein)               :: 2,889
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 36 of 73
            Pseudo Genes (incomplete)         :: 29 of 73
            Pseudo Genes (internal stop)      :: 18 of 73
            Pseudo Genes (multiple problems)  :: 8 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..802
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639966-rid9879223.denovo.223"
                     /isolate="E20313_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            <1..>802
                     /locus_tag="IWO89_002936"
     CDS             <1..>802
                     /locus_tag="IWO89_002936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0457611.1"
                     /translation="LLDAPYYLSKVALGENLIKPESQQPVTNSNYLINTYNIKPMVKG
                     KKYTITLEGTKPATQVFRPFFTRATGDAWEVGDLQPVEGLTDVWSGTFTAAADPHPTS
                     PQVQICQVPSTSLGNCTIKWLKLEEGDTRTPNIEQYKYRGIGMRDSNNPKDYVWDLAP
                     EYVEDNLATDIKISEITGKANNYTDGKVSEINSQLTASINEVDTTAKDAQTKANANAT
                     AIDELDNKIDERINDTATTTLTVTNGNTGSAKLYREGKTVSIYFVALNG"
BASE COUNT          296 a          154 c          160 g          192 t
ORIGIN      
        1 ctacttgatg caccgtatta tttgagtaag gtggctttgg gtgagaatct aattaaacca
       61 gaatcacaac aaccagttac taatagtaac tatcttatta acacctataa cattaaacca
      121 atggtaaaag gtaagaagta taccatcaca cttgaaggaa ctaagccagc aacacaggtt
      181 tttagaccat ttttcacacg agcaacagga gatgcatggg aggttggtga cttacaacca
      241 gtagaagggt taactgatgt ttggtctgga acatttacag cagcagctga cccacaccct
      301 accagcccac aagttcagat ttgtcaggta ccaagcacaa gtttaggaaa ctgtacaatt
      361 aaatggttaa aactagagga aggcgacaca cgaaccccga atattgagca atataaatac
      421 cgaggaatcg gcatgcgaga ctcaaacaat ccaaaagatt acgtctggga tctagcacca
      481 gaatatgtcg aagacaatct ggccacagat attaaaattt ctgaaattac tggtaaagca
      541 aacaattata ccgatgggaa agtatcggag attaattcgc agttgactgc ttcaattaat
      601 gaagtagaca ccacagctaa ggatgctcaa acaaaagcga atgctaatgc gactgctata
      661 gatgaattag acaataagat cgatgaacgc attaatgata cagctactac cacattaaca
      721 gttacaaacg ggaataccgg atcagcaaag ctttatcgtg aaggaaaaac agtttctata
      781 tattttgtgg ctttaaacgg aa
//
LOCUS       DACEEZ010000212          744 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20313_2017
            SAMN12639966-rid9879223.denovo.224, whole genome shotgun sequence.
ACCESSION   DACEEZ010000212 DACEEZ010000000
VERSION     DACEEZ010000212.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639966
            Sequence Read Archive: SRR10023158
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 744)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 744)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741683.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741683.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:44:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,016
            CDSs (total)                      :: 2,962
            Genes (coding)                    :: 2,889
            CDSs (with protein)               :: 2,889
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 36 of 73
            Pseudo Genes (incomplete)         :: 29 of 73
            Pseudo Genes (internal stop)      :: 18 of 73
            Pseudo Genes (multiple problems)  :: 8 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..744
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639966-rid9879223.denovo.224"
                     /isolate="E20313_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            2..>744
                     /locus_tag="IWO89_002937"
     rRNA            2..>744
                     /locus_tag="IWO89_002937"
                     /product="23S ribosomal RNA"
BASE COUNT          230 a          146 c          221 g          147 t
ORIGIN      
        1 attgtgtgaa tacatagcac attcgaggta gacgcagaga actgaaacat ctaagtacct
       61 gcaggaagag aaagaaaatt cgattccctg agtagcggcg agcgaaacgg gaaaagccca
      121 aaccagcaag cttgcttgtt ggggttgtag gactccaata tggtagttct ttcagatagt
      181 cgaatgactt ggaaaagtca gtcaaagagg gtaaaaaccc cgtagacgaa atgtggaaga
      241 cacctaggag gatcctgagt acggcggaac acgagaaatt ccgtcggaat ccgggaggac
      301 catctcccaa ggctaaatac tccctagtga ccgatagtga accagtaccg tgagggaaag
      361 gtgaaaagca ccccggaagg ggagtgaaat agaacctgaa accgtgtgcc tacaacaagt
      421 caaagcccgt taatgggtga tggcgtgcct tttgtagaat gaaccggcga gttacgattg
      481 catgcgaggt taagttgaag agacggagcc gcagcgaaag cgagtctgaa tagggcgttt
      541 gagtatgtag tcgtagaccc gaaaccatgt gatctaccca tgtccaggtt gaaggtgcgg
      601 taaaacgcac tggaggaccg aacccacgta cgttgaaaag tgcggggatg aggtgtgggt
      661 agcggagaaa ttccaaacga acttggagat agctggttct ctccgaaata gctttagggc
      721 tagcctcgga attgagaatg atgg
//
LOCUS       DACEEZ010000213          712 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20313_2017
            SAMN12639966-rid9879223.denovo.225, whole genome shotgun sequence.
ACCESSION   DACEEZ010000213 DACEEZ010000000
VERSION     DACEEZ010000213.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639966
            Sequence Read Archive: SRR10023158
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 712)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 712)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741683.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741683.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:44:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,016
            CDSs (total)                      :: 2,962
            Genes (coding)                    :: 2,889
            CDSs (with protein)               :: 2,889
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 36 of 73
            Pseudo Genes (incomplete)         :: 29 of 73
            Pseudo Genes (internal stop)      :: 18 of 73
            Pseudo Genes (multiple problems)  :: 8 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..712
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639966-rid9879223.denovo.225"
                     /isolate="E20313_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(<1..446)
                     /locus_tag="IWO89_002938"
     CDS             complement(<1..446)
                     /locus_tag="IWO89_002938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M protein trans-acting positive regulator"
                     /protein_id="HAQ0457612.1"
                     /translation="MNYEFFLSKEDHRSYSLLKYLEASSNLSESISNVQEELSLSTFL
                     LKKTIDKLKHDLQKFDLEKNLRVTVSELDICLEINGKCSSKALLSRYITDSLSMKMVL
                     AFFQEKYISVENFAKQHHVSYSVAYKVLQRLKRNLKKYQIFFEKRK"
BASE COUNT          216 a          123 c           95 g          278 t
ORIGIN      
        1 aactttctct tttcaaaaaa aatttgatat ttttttagat ttctttttag tctttgtaat
       61 actttataag ccacagaata actcacgtga tgctgtttag caaaattttc tactgagata
      121 tatttttcct gaaagaaagc caataccatt ttcatagata aagaatccgt tatataacga
      181 gataacagag ctttgctaga acattttcca ttaatttcca gacatatatc caattcactg
      241 acggttactc gcaaattttt ctctagatcg aacttttgta aatcatgttt cagcttatcg
      301 atcgtttttt tcaagagaaa tgtcgatagc gacaactctt cttgaacatt cgatattgac
      361 tctgataaat ttgagcttgc ttctaaatat ttaagtaatg aataacttct gtgatcttct
      421 ttgcttaaaa aaaactcata attcattagt catcagctct ttctacgtat attactttcc
      481 atgcattcac caactagctg gggataaaac tgatttcctg gatccgagtc actgttgtca
      541 gcgattcttg tttgtataat atacatcgtc ctgtcttttt tttaacattt tggagagcgt
      601 tttatttggc ctttctcgcg atcaaacctg ttattagaat tatatcattt actagtatgc
      661 atttttatta ataaaatggt atattttata ttgttataaa aagagcccaa cc
//
LOCUS       DACEEZ010000214          704 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20313_2017
            SAMN12639966-rid9879223.denovo.226, whole genome shotgun sequence.
ACCESSION   DACEEZ010000214 DACEEZ010000000
VERSION     DACEEZ010000214.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639966
            Sequence Read Archive: SRR10023158
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 704)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 704)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741683.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741683.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:44:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,016
            CDSs (total)                      :: 2,962
            Genes (coding)                    :: 2,889
            CDSs (with protein)               :: 2,889
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 36 of 73
            Pseudo Genes (incomplete)         :: 29 of 73
            Pseudo Genes (internal stop)      :: 18 of 73
            Pseudo Genes (multiple problems)  :: 8 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..704
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639966-rid9879223.denovo.226"
                     /isolate="E20313_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            94..>704
                     /locus_tag="IWO89_002939"
     CDS             94..>704
                     /locus_tag="IWO89_002939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HAQ0457613.1"
                     /translation="MHETGIDAPARNVAANMKNNYNGSNSYTTDVIGDGGIVYRVGEQ
                     GYVSWGAGNANPYAPVQIELQRTYDKALFEKNYRAYIEYTRDSAKKYGIPLTLDQGTS
                     LFTKGIISHLWVTNYVWGNHTDPYGYLSQMGVSKEKLAYDLAHGFTDENPTTSENKPV
                     IDPTRAGAANPTLTDGTNHAHIDQFGEIENANLHVAGWHIANY"
BASE COUNT          238 a          127 c          146 g          193 t
ORIGIN      
        1 cccattaata cgtttgctta tactattaat gacgagtata atttagcgcc gaatcaagga
       61 gactccagat tagcaattcc taacaaaatt attttgcacg aaactggaat agatgcacca
      121 gcaagaaacg tagccgccaa catgaaaaat aattataacg gaagcaattc ttatactaca
      181 gatgttattg gtgacggtgg gattgtttac cgtgtgggtg agcaaggata tgtttcgtgg
      241 ggagctggta acgctaatcc ttatgcgcct gtacagattg aattacagcg cacatatgat
      301 aaagcattgt ttgaaaaaaa ctatcgagct tatattgaat atacaagaga tagtgcaaaa
      361 aaatatggaa ttccattgac tcttgatcaa ggaacttctt tatttacaaa aggaatcatt
      421 tctcatttgt gggtgacaaa ttatgtttgg gggaaccaca cagatccata tggttactta
      481 tcgcaaatgg gagttagcaa agaaaagctt gcttatgatt tagctcatgg atttaccgat
      541 gaaaatccaa caacttctga aaacaagcct gtcattgatc caacacgagc tggtgcagct
      601 aatcctacgc tgacagatgg aacaaatcac gcccacattg atcagtttgg ggaaatcgaa
      661 aacgcaaact tgcatgtggc tggatggcac attgctaact ataa
//
LOCUS       DACEEZ010000215          697 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20313_2017
            SAMN12639966-rid9879223.denovo.227, whole genome shotgun sequence.
ACCESSION   DACEEZ010000215 DACEEZ010000000
VERSION     DACEEZ010000215.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639966
            Sequence Read Archive: SRR10023158
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 697)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 697)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741683.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741683.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:44:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,016
            CDSs (total)                      :: 2,962
            Genes (coding)                    :: 2,889
            CDSs (with protein)               :: 2,889
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 36 of 73
            Pseudo Genes (incomplete)         :: 29 of 73
            Pseudo Genes (internal stop)      :: 18 of 73
            Pseudo Genes (multiple problems)  :: 8 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..697
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639966-rid9879223.denovo.227"
                     /isolate="E20313_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            <1..>697
                     /locus_tag="IWO89_002940"
     CDS             <1..>697
                     /locus_tag="IWO89_002940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ISL3-like element ISEfa5 family transposase"
                     /protein_id="HAQ0457614.1"
                     /translation="QKKKTLIVDAVFSPAPHTCRNCGSTVVDGNGKVIVVKNGKKETI
                     VRFEQYNHMPLVMRLKKQRYTCKNCRTHWTAQSYFVQPRHSIANHVRYKIASLLTEKV
                     SLSFIAKNCQVSLTTVIRTLKEFKSYLPKQSKRILPRVLMVDEFRSHASIEDKMSFIC
                     ADGETGQLIDVLPTRKLPRLTSYFLGCANPEEVKFLVTDMNAAYFQLTKRILPNAKVV
                     IDRFHIIKHMNQA"
BASE COUNT          219 a          136 c          136 g          206 t
ORIGIN      
        1 ttcagaagaa aaagacgttg atcgtcgatg ctgttttctc acctgctccc catacctgta
       61 gaaattgtgg ttctactgtg gtagatggaa acgggaaagt aattgtagtg aaaaatggga
      121 agaaggaaac gattgtccgc tttgaacaat acaatcacat gcctttggtt atgcgcttaa
      181 aaaagcagcg ctatacctgt aaaaactgtc gaacccattg gacggctcaa agttattttg
      241 ttcaacccag acattcaatc gcgaatcatg tcagatataa aattgcttct ttactgactg
      301 aaaaagtatc tttatctttt attgcgaaaa attgtcaggt atctttgacc accgttattc
      361 gtacgttgaa agagtttaaa agctacttac caaagcaatc taagaggatt ctccccagag
      421 tattgatggt tgatgaattt cgttcgcacg cttccataga agataaaatg agctttattt
      481 gcgcagatgg cgaaacagga caattaattg atgtcttgcc tacgcgtaaa ttgcctcgat
      541 taacaagcta tttcttaggc tgcgctaatc ctgaagaagt aaaattttta gtcacagaca
      601 tgaacgccgc ctacttccag cttaccaaac gtattctgcc aaatgcgaaa gtagtgattg
      661 atcggtttca tattatcaaa cacatgaatc aagcctt
//
LOCUS       DACEEZ010000216          695 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20313_2017
            SAMN12639966-rid9879223.denovo.228, whole genome shotgun sequence.
ACCESSION   DACEEZ010000216 DACEEZ010000000
VERSION     DACEEZ010000216.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639966
            Sequence Read Archive: SRR10023158
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 695)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 695)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741683.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741683.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:44:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,016
            CDSs (total)                      :: 2,962
            Genes (coding)                    :: 2,889
            CDSs (with protein)               :: 2,889
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 36 of 73
            Pseudo Genes (incomplete)         :: 29 of 73
            Pseudo Genes (internal stop)      :: 18 of 73
            Pseudo Genes (multiple problems)  :: 8 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..695
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639966-rid9879223.denovo.228"
                     /isolate="E20313_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(53..628)
                     /locus_tag="IWO89_002941"
     CDS             complement(53..628)
                     /locus_tag="IWO89_002941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HAQ0457615.1"
                     /translation="MNKNKMATRVLTVMALCIGINYIGGTIALWLRLPIYLDSIGTIF
                     AGALLGPVPGMLTGLSSGILSGVTTDIFSLYYSPIQILTGLLAGLILPRYLGKDKNSL
                     KLLLLALILSVPGTIVSSIITVQLFGGITSSGSSMIVQLLHGLGLSQTTSVILVQAGT
                     DYLDRLLSIVVVSLVITMLPQRLIYSKRKIG"
BASE COUNT          229 a          151 c          124 g          191 t
ORIGIN      
        1 atgaaaaaac agagccagat tagcattatt ttgctaatcc agctctgttt attcagccta
       61 tttttctttt tgaatatatc aagcgttggg gcaacatagt gatgactaat gaaacaacga
      121 cgatagacaa caagcgatca aggtagtctg tacccgcctg taccaagatc acacttgttg
      181 tctgacttaa acctaaccca tgtaatagct gaacgatcat actggatcct gaggaggtga
      241 tcccgccaaa taactgaacg gtaatgatcg aagaaacgat cgttccaggt accgacaaaa
      301 tcaaagcgag taataacagt ttcaagctgt ttttatcttt gccgagatat cttggcaaaa
      361 tcaatccagc aagcaagcct gtgagaattt ggattggcga ataatacaga gaaaaaatat
      421 ctgtagtcac tccacttaaa ataccgctgg acaatcctgt cagcattcca ggtacaggcc
      481 caagcaatgc accagcaaaa atcgttccga tagaatcgag atagattggc aaacgaagcc
      541 ataaagcaat cgttcctcca atataattga tcccaataca caaagccatc actgtaagta
      601 cgcgagttgc cattttgttt ttattcattt tgttagccca tctttaacct ttccaaatct
      661 ttcataatcg tttcttcttg tgcatttaaa agaac
//
LOCUS       DACEEZ010000217          694 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20313_2017
            SAMN12639966-rid9879223.denovo.229, whole genome shotgun sequence.
ACCESSION   DACEEZ010000217 DACEEZ010000000
VERSION     DACEEZ010000217.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639966
            Sequence Read Archive: SRR10023158
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 694)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 694)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741683.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741683.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:44:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,016
            CDSs (total)                      :: 2,962
            Genes (coding)                    :: 2,889
            CDSs (with protein)               :: 2,889
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 36 of 73
            Pseudo Genes (incomplete)         :: 29 of 73
            Pseudo Genes (internal stop)      :: 18 of 73
            Pseudo Genes (multiple problems)  :: 8 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..694
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639966-rid9879223.denovo.229"
                     /isolate="E20313_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(91..>694)
                     /locus_tag="IWO89_002942"
     CDS             complement(91..>694)
                     /locus_tag="IWO89_002942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS3-like element ISEfa8 family transposase"
                     /protein_id="HAQ0457616.1"
                     /translation="FKGQRTACNEAKTANVLDRTFTQEQPLEAIVTDLTYVRVGKKWH
                     YICLILDLFNREIIGYSCGEKKDASLVKEAFGRIPYSLTDVKLFHTDRGKEFDNQTIH
                     EILNGFGITRSLSRKGCPYDNAVVESTYKSVKVEFVHQYQFETLAQLRLELFDYVHWW
                     NYLRLHGTLAYETPIQIRQQRLAKRILDNERGSDTSGEAA"
BASE COUNT          192 a          163 c          127 g          212 t
ORIGIN      
        1 ttgtgaatgc tgcggtgtta tggctacgat tgcccttgac aatgagcatc ctcggatctg
       61 attttctccg gcagaagcac aatcattcaa ttacgctgcc tctccagagg tatcagatcc
      121 gcgctcatta tcaaggattc gcttcgccaa tctctgttgt cgaatttgga tcggtgtttc
      181 atacgccaac gtgccatgta agcgtagata gttccaccaa tgcacataat caaacaattc
      241 tagacgtagc tgtgccagtg tctcaaattg gtattgatgc acgaattcta ctttgacaga
      301 tttataggtt gattccacaa cggcattatc atacggacaa cccttcctac tcaatgaacg
      361 agtaattcca aaaccattca gaatctcatg aatggtttgg ttatcaaatt cctttccccg
      421 gtctgtatga aaaagcttga cgtctgttaa agaatacggt atccgtccaa aggcttcttt
      481 taccaatgag gcatctttct tctcaccaca ggaataacca ataatttctc gattaaacaa
      541 atcaagtatt aagcagatat aatgccactt tttccccacg cgaacataag taagatccgt
      601 aacgatggct tccaatggct gttcttgtgt aaaggtccga tctaatacat tcgctgtttt
      661 cgcttcatta caagctgttc gttgcccttt aaaa
//
LOCUS       DACEEZ010000218          663 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20313_2017
            SAMN12639966-rid9879223.denovo.230, whole genome shotgun sequence.
ACCESSION   DACEEZ010000218 DACEEZ010000000
VERSION     DACEEZ010000218.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639966
            Sequence Read Archive: SRR10023158
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 663)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 663)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741683.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741683.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:44:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,016
            CDSs (total)                      :: 2,962
            Genes (coding)                    :: 2,889
            CDSs (with protein)               :: 2,889
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 36 of 73
            Pseudo Genes (incomplete)         :: 29 of 73
            Pseudo Genes (internal stop)      :: 18 of 73
            Pseudo Genes (multiple problems)  :: 8 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..663
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639966-rid9879223.denovo.230"
                     /isolate="E20313_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            <1..>663
                     /locus_tag="IWO89_002943"
     CDS             <1..>663
                     /locus_tag="IWO89_002943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ISL3-like element ISEfa11 family transposase"
                     /protein_id="HAQ0457617.1"
                     /translation="QKKKTLVVDAVLSPTPRACRSCGSTVVDGNGKAIIVKNGKKETI
                     VRFEQYNHMPLVMRLKKQRYTCKNCRTHWTAQSYFVQPRHSIANHVRYKIASLLTEKV
                     SLSFIAKSCQVSLTTVIRTLKEFKSYLPKQSKKILPRVLMVDEFRSHASIEDKMSFIC
                     ADGETGKLIDVLPTRKLPRLTSYFLGCTNPEEVEFLVTDMNAAYFQLTKRVLPNAKVV
                     ID"
BASE COUNT          207 a          129 c          134 g          193 t
ORIGIN      
        1 ttcagaagaa aaagacgttg gtcgtcgatg ctgttctctc gcctactcct cgtgcttgta
       61 gaagttgtgg ttctactgtg gtagatggaa acgggaaagc aattatagtg aaaaatggaa
      121 aaaaggaaac gattgtccgt tttgaacaat acaatcatat gcctttggtt atgcgcctaa
      181 aaaagcaacg ctatacctgt aaaaactgtc gcacccattg gacggctcaa agttattttg
      241 tccaacccag acattcaatc gcaaatcatg ttagatataa aattgcttct ttactgactg
      301 aaaaagtatc tttatctttt attgcgaaaa gctgtcaggt atctttgacc accgttattc
      361 gtacattgaa agagtttaaa agctatttac caaagcaatc taagaagatc ctccccagag
      421 tattgatggt tgacgaattt cgttcgcatg cttccataga agataagatg agctttattt
      481 gcgcggatgg cgaaacagga aaattaatag atgttttgcc tacgcgtaaa ttacctcgat
      541 taacaagcta tttcttaggt tgtaccaatc cagaagaagt agaattcttg gtgacagaca
      601 tgaacgccgc ctacttccag ctcaccaaac gtgttctgcc aaatgcgaaa gtcgtgattg
      661 atc
//
LOCUS       DACEEZ010000219          616 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20313_2017
            SAMN12639966-rid9879223.denovo.231, whole genome shotgun sequence.
ACCESSION   DACEEZ010000219 DACEEZ010000000
VERSION     DACEEZ010000219.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639966
            Sequence Read Archive: SRR10023158
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 616)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 616)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741683.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741683.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:44:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,016
            CDSs (total)                      :: 2,962
            Genes (coding)                    :: 2,889
            CDSs (with protein)               :: 2,889
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 36 of 73
            Pseudo Genes (incomplete)         :: 29 of 73
            Pseudo Genes (internal stop)      :: 18 of 73
            Pseudo Genes (multiple problems)  :: 8 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..616
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639966-rid9879223.denovo.231"
                     /isolate="E20313_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            51..>616
                     /locus_tag="IWO89_002944"
     CDS             51..>616
                     /locus_tag="IWO89_002944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_064305258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS6-like element IS1216 family transposase"
                     /protein_id="HAQ0457618.1"
                     /translation="MNHFKGKQFQQDVIIVAVGYYLRYNLSYREVQEILYDRGINVSH
                     TTIYRWVQEYGKLLYQIWKKKNKKSFYSWKMDETYIKIKGKWHYLYRAIDADGLTLDI
                     WLRKKRDTQAAYAFLKRLVKQFDEPKVVVTDKAPSITSAFKKLKEYGFYQGTEHRTIK
                     YLNNLIEQDHRPVKRRNKFYRSLRTASTT"
BASE COUNT          223 a          103 c          120 g          170 t
ORIGIN      
        1 tactatcaaa taaggtagaa taatagaaaa agatagcagg aggaatgacg atgaatcatt
       61 ttaaaggaaa gcaatttcag caggatgtga ttattgtagc cgtgggctac tatcttcgtt
      121 ataaccttag ctatcgtgaa gttcaagaaa tcttatatga tcgtggcatt aacgtttctc
      181 atacgacgat ttatcgttgg gtgcaagaat atggcaaact actctatcaa atttggaaaa
      241 agaaaaataa aaaatccttt tattcatgga aaatggatga aacgtacatc aaaattaaag
      301 gaaaatggca ttatttgtat cgagccatcg atgcagatgg tttaaccttg gatatttggt
      361 tacgtaaaaa acgggacaca caagcagcct atgcttttct taagcggtta gtgaagcagt
      421 ttgatgaacc gaaggttgta gtcacagata aagccccctc tattacaagt gcctttaaga
      481 aactaaaaga atacggcttt tatcaaggga cagaacatcg taccattaaa tacctgaata
      541 atttgattga acaagaccat cgtccagtaa agagacgcaa taaattctat cgaagtttac
      601 gcactgcctc taccac
//
LOCUS       DACEEZ010000220          591 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20313_2017
            SAMN12639966-rid9879223.denovo.232, whole genome shotgun sequence.
ACCESSION   DACEEZ010000220 DACEEZ010000000
VERSION     DACEEZ010000220.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639966
            Sequence Read Archive: SRR10023158
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 591)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 591)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741683.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741683.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:44:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,016
            CDSs (total)                      :: 2,962
            Genes (coding)                    :: 2,889
            CDSs (with protein)               :: 2,889
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 36 of 73
            Pseudo Genes (incomplete)         :: 29 of 73
            Pseudo Genes (internal stop)      :: 18 of 73
            Pseudo Genes (multiple problems)  :: 8 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..591
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639966-rid9879223.denovo.232"
                     /isolate="E20313_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            1..591
                     /locus_tag="IWO89_002945"
                     /pseudo
     CDS             1..591
                     /locus_tag="IWO89_002945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303667.1"
                     /note="internal stop; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="transposase"
BASE COUNT          176 a          131 c          116 g          168 t
ORIGIN      
        1 ctttcatgaa gatgcctttc gcaaagagcg aatcaatgac gaaacatgtt ttgtttttga
       61 tggcacattg acctatcaac cggaggaatg ctttcatcgc cactatcaaa atgaacaaac
      121 gatcattaaa tagggctgga aaaaagtatc catcttattg aatgatgtca gtaactacaa
      181 gaccattctg cggattaaca aacagcgctt caaatgtaaa cactgtggaa agactttttt
      241 agcagaagat tcagttagtg atcgtcgctg ctccattgct cgcagagtca aacaagccat
      301 tcttgagcta ttaagcgaac ctctttctat gtctttgatc gcacgtatga agcacatttc
      361 tccgaccact gtcattcgaa tacttcgcag ccttcggcct aaaaccattt ctttgagcca
      421 gctgttacca gaagtggtgt gtttcgatga attcaaatca gtgaaaaatg tctcgggagc
      481 catgagcttt gtcatgatgg acggcaagac ccatcaactg attgatattg tggaaaatcg
      541 gcaactcaat cctttacgcg actatttctt gcgctaccct ataaaagtcc g
//
LOCUS       DACEEZ010000221          581 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20313_2017
            SAMN12639966-rid9879223.denovo.233, whole genome shotgun sequence.
ACCESSION   DACEEZ010000221 DACEEZ010000000
VERSION     DACEEZ010000221.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639966
            Sequence Read Archive: SRR10023158
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 581)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 581)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741683.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741683.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:44:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,016
            CDSs (total)                      :: 2,962
            Genes (coding)                    :: 2,889
            CDSs (with protein)               :: 2,889
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 36 of 73
            Pseudo Genes (incomplete)         :: 29 of 73
            Pseudo Genes (internal stop)      :: 18 of 73
            Pseudo Genes (multiple problems)  :: 8 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..581
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639966-rid9879223.denovo.233"
                     /isolate="E20313_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            4..360
                     /locus_tag="IWO89_002946"
     CDS             4..360
                     /locus_tag="IWO89_002946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1140 family protein"
                     /protein_id="HAQ0457619.1"
                     /translation="MDLITQYSDIILKKIMMKIQKDKKSKERAELVKLEMAETGAGVR
                     SSRHWKAAANIEFYYNEIQKGFDQMRELDRQTNWSKKLHQDRFKFVEKYKEILEEYLR
                     RTANDKKTRSIQHGFI"
     gene            320..565
                     /locus_tag="IWO89_002947"
     CDS             320..565
                     /locus_tag="IWO89_002947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0457620.1"
                     /translation="MIKKLVQFSMDLYDIESGATLSVESDHLIINFGGKRQIILWVVD
                     DVLFPEIVHDFEESKAVEFEIVKKVMELIEKYEEDSE"
BASE COUNT          228 a           75 c          133 g          145 t
ORIGIN      
        1 ataatggatc tcattacaca atacagtgac atcatcctca agaaaatcat gatgaagatt
       61 cagaaagata aaaaatcaaa agaacgagct gaattagtta agttagaaat ggctgaaaca
      121 ggagcaggag tgcgaagtag caggcattgg aaagcagcag caaacattga attttattac
      181 aacgaaattc aaaaagggtt cgatcagatg cgtgagctgg atcggcaaac aaattggagc
      241 aagaaacttc atcaagatcg tttcaaattt gtagagaagt ataaagaaat attagaagag
      301 tatttgagga ggacagcaaa tgataaaaaa actcgttcaa ttcagcatgg atttatatga
      361 tatcgaatca ggagcaacac tatctgtgga atcggaccat ctaatcataa attttggtgg
      421 aaagcgccag attattttgt gggtagttga tgatgtactg tttccagaaa ttgttcatga
      481 tttcgaagaa tcaaaagcgg ttgagtttga aatagtgaaa aaagtaatgg aattgattga
      541 aaaatacgag gaggacagcg aatgataccg aagtttagag c
//
LOCUS       DACEEZ010000222          540 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20313_2017
            SAMN12639966-rid9879223.denovo.234, whole genome shotgun sequence.
ACCESSION   DACEEZ010000222 DACEEZ010000000
VERSION     DACEEZ010000222.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639966
            Sequence Read Archive: SRR10023158
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 540)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 540)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741683.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741683.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:44:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,016
            CDSs (total)                      :: 2,962
            Genes (coding)                    :: 2,889
            CDSs (with protein)               :: 2,889
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 36 of 73
            Pseudo Genes (incomplete)         :: 29 of 73
            Pseudo Genes (internal stop)      :: 18 of 73
            Pseudo Genes (multiple problems)  :: 8 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..540
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639966-rid9879223.denovo.234"
                     /isolate="E20313_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            <1..75
                     /locus_tag="IWO89_002948"
     CDS             <1..75
                     /locus_tag="IWO89_002948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage regulatory protein"
                     /protein_id="HAQ0457621.1"
                     /translation="PETAIRMEIDQLNRQQRLFGDENE"
     gene            249..>540
                     /locus_tag="IWO89_002949"
     CDS             249..>540
                     /locus_tag="IWO89_002949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0457622.1"
                     /translation="MNKELSTLDQYLTDSEWGKSNIKETNNRKIRRNLLTNEELSCDQ
                     DDLGNFVSIWDHVYLIHLSKHSNKPEYIYVIEDGLIDALEEYNRDNLIDISYY"
BASE COUNT          212 a           94 c          104 g          130 t
ORIGIN      
        1 ccagaaactg cgatccgcat ggaaatcgat caactgaaca gacaacaaag acttttcggt
       61 gatgaaaatg aatagagctg aagcgctaag aatagggacg gtaattgcta atcgctggtg
      121 gagacacaat aaaccaaaca tcctaagcca acaacatatc gaaaagcaaa aagcatggca
      181 acaaataaaa aagtgactca gccggcaagc atagagtcac aaaacatatc taaggagaat
      241 tttagcatat gaataaagaa ctttccactt tagatcaata tttgactgat tctgaatggg
      301 gcaagtcgaa tatcaaggaa acaaataatc gaaaaatcag acgaaatctt ttgacgaacg
      361 aagaactatc atgtgatcaa gatgacttgg gaaattttgt gagtatttgg gatcatgttt
      421 accttatcca tctatcaaaa cattcaaata aacctgaata catctatgtc atcgaagatg
      481 gcttgattga tgcgctagaa gagtacaaca gagataactt gattgatatc tcttattacg
//
LOCUS       DACEEZ010000223          538 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20313_2017
            SAMN12639966-rid9879223.denovo.235, whole genome shotgun sequence.
ACCESSION   DACEEZ010000223 DACEEZ010000000
VERSION     DACEEZ010000223.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639966
            Sequence Read Archive: SRR10023158
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 538)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 538)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741683.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741683.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:44:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,016
            CDSs (total)                      :: 2,962
            Genes (coding)                    :: 2,889
            CDSs (with protein)               :: 2,889
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 36 of 73
            Pseudo Genes (incomplete)         :: 29 of 73
            Pseudo Genes (internal stop)      :: 18 of 73
            Pseudo Genes (multiple problems)  :: 8 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..538
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639966-rid9879223.denovo.235"
                     /isolate="E20313_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(18..371)
                     /gene="tnpB"
                     /locus_tag="IWO89_002950"
     CDS             complement(18..371)
                     /gene="tnpB"
                     /locus_tag="IWO89_002950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001846105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HAQ0457623.1"
                     /translation="MLYDLLQTKHIYIVCGKTDLRKGIDGLASLIQQEYQLELYEDAV
                     FLFCGNRQDRFKLLYWDGDGFLLCYKRIENGKLKWPRTKDEVRTLTNQQVRWLLEGLS
                     IDQPRAILPGKKGVF"
BASE COUNT          155 a          120 c           92 g          171 t
ORIGIN      
        1 tgtgttaacc ccccagctta aaaaactcct ttttttcctg gcaaaatagc tcgaggttga
       61 tcgatactta atccttctaa caaccatcga acctgttggt tcgttaaggt tcgaacttcg
      121 tctttcgtcc gaggccattt taacttgccg ttttctatcc gcttataaca gagaagaaaa
      181 ccatcaccgt cccaatagag aagtttgaaa cggtcttgac ggttcccgca aaataaaaaa
      241 acggcatctt cgtaaagctc gagttgatac tcttgttgaa taagactggc aagaccatca
      301 attccttttc gcaaatctgt ttttccgcac acaatataaa tatgtttggt ttgaagtaaa
      361 tcataaagca ttggacaaca gttctttcaa aatagcttga agaatataat tgttaatacc
      421 gttatagatc aaaacttcag aaccattctc tcttttgatg cgcatcgcta acttggcagc
      481 cgcgggttta cctttttctg gtgcactctt ttcgtttcct aattggacag gaacaatc
//
LOCUS       DACEEZ010000224          514 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20313_2017
            SAMN12639966-rid9879223.denovo.236, whole genome shotgun sequence.
ACCESSION   DACEEZ010000224 DACEEZ010000000
VERSION     DACEEZ010000224.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639966
            Sequence Read Archive: SRR10023158
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 514)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 514)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741683.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741683.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:44:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,016
            CDSs (total)                      :: 2,962
            Genes (coding)                    :: 2,889
            CDSs (with protein)               :: 2,889
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 36 of 73
            Pseudo Genes (incomplete)         :: 29 of 73
            Pseudo Genes (internal stop)      :: 18 of 73
            Pseudo Genes (multiple problems)  :: 8 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..514
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639966-rid9879223.denovo.236"
                     /isolate="E20313_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(107..>514)
                     /locus_tag="IWO89_002951"
     CDS             complement(107..>514)
                     /locus_tag="IWO89_002951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_465591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="linear amide C-N hydrolase"
                     /protein_id="HAQ0457624.1"
                     /translation="GTPENHFSNQISLNVYSRGMGGLGLPGDLSSVSRFVKATFTKMN
                     AASGDSESESISQFFHILGSVEQQKGVCDTGEGKYEYTIYSSCCNVDKGIYYYRTYED
                     SQITAVNMNKEDLDRHELVSYPVIKEQQINYVN"
BASE COUNT          162 a          103 c           78 g          171 t
ORIGIN      
        1 atcatccata aaatggactt tgtctacagt ctgagacacg acctaaagat cgtgtctaat
       61 tatgactgta tcagaaaata tgataccaaa cggatctatt cattaattaa ttgacatagt
      121 ttatctgttg ctcttttatg actggataac tgaccaattc atgacgatcc aagtcttctt
      181 tattcatatt tacagcagta atttgactgt cttcatacgt acgataataa taaatccctt
      241 tatcaacgtt acagcaagaa gaataaatcg tatattcgta ttttccttct ccagtatcgc
      301 atactccttt ttgttgctcg actgagccta gtatatgaaa aaattgactg atactttcag
      361 attctgaatc acctgatgca gcattcattt tagtaaatgt ggcttttaca aatcgagaaa
      421 cagaagagag atctccaggt aagcccaatc ctcccatccc acgactataa acattcaagc
      481 tgatctggtt tgaaaaatga ttttctggtg ttcc
//
LOCUS       DACEEZ010000225          508 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20313_2017
            SAMN12639966-rid9879223.denovo.237, whole genome shotgun sequence.
ACCESSION   DACEEZ010000225 DACEEZ010000000
VERSION     DACEEZ010000225.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639966
            Sequence Read Archive: SRR10023158
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 508)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 508)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741683.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741683.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:44:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,016
            CDSs (total)                      :: 2,962
            Genes (coding)                    :: 2,889
            CDSs (with protein)               :: 2,889
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 36 of 73
            Pseudo Genes (incomplete)         :: 29 of 73
            Pseudo Genes (internal stop)      :: 18 of 73
            Pseudo Genes (multiple problems)  :: 8 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..508
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639966-rid9879223.denovo.237"
                     /isolate="E20313_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
BASE COUNT          228 a           70 c           81 g          129 t
ORIGIN      
        1 aattccttag gcagcttttg ctgtgtagaa aggaataaat aataacgaaa gttcaaaagc
       61 taatgccgac tattctagac attaaagaag acgaaaacga acgataaaga aagaaaagtt
      121 atgaataatc gtttattgaa aaaaacttga aaaaatacaa aaaattcgtt gaccaagcta
      181 aatttagttg gtatattatt acttgtccaa aagacagaag aaaatattta ttcaaaaaca
      241 taacaaaaaa gttattgaca tgttactaac gacatgataa gataactgag tcgttaaaaa
      301 agaaccactt gcgaagcttg attctatcga aaaaaacttc aaaaaagttc ttgacaaata
      361 acaaacgata agttatgata aatgagttgc cgaaagattt cgaaaaacga aatcgaaaaa
      421 aacttttaaa aaagagttga caatcgcttg tctctctgat atgatataaa agttgctacg
      481 gcagccaaaa atgaatcggg aaacactc
//
LOCUS       DACEEZ010000226          506 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20313_2017
            SAMN12639966-rid9879223.denovo.238, whole genome shotgun sequence.
ACCESSION   DACEEZ010000226 DACEEZ010000000
VERSION     DACEEZ010000226.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639966
            Sequence Read Archive: SRR10023158
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 506)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 506)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741683.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741683.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:44:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,016
            CDSs (total)                      :: 2,962
            Genes (coding)                    :: 2,889
            CDSs (with protein)               :: 2,889
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 36 of 73
            Pseudo Genes (incomplete)         :: 29 of 73
            Pseudo Genes (internal stop)      :: 18 of 73
            Pseudo Genes (multiple problems)  :: 8 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..506
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639966-rid9879223.denovo.238"
                     /isolate="E20313_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            231..>506
                     /locus_tag="IWO89_002952"
     CDS             231..>506
                     /locus_tag="IWO89_002952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002419316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAQ0457625.1"
                     /translation="MTYKHLTIDELTMIESYYLQHNKPVEIANRMGRAIQTIYNVVNK
                     FKQGKTALDYWHQYKENKKKCGRKVIQLPAHEVDYIKEKVTLGWTPDV"
BASE COUNT          190 a           83 c           88 g          145 t
ORIGIN      
        1 cgatagaatc aagcttcgca agtggttctt tttaacgact tagttatctt atcacgtcta
       61 aaaacatgtg tcaactcttt tttggaattt tcataaaatg tttcaatgaa gcgcgacaac
      121 gttgtttaat atatcgcgaa ttgtaaaatt aagttagaaa aataaaaagg catttatggt
      181 acactcaaat tgtatttccg acgaaagaaa acaaaggagt gtaaccataa atgacctaca
      241 aacatcttac catagacgaa ctgacaatga tagaatcata ttatcttcaa cataataagc
      301 cggttgaaat cgctaaccga atgggacgtg ctatacaaac tatttataat gtagtcaata
      361 agttcaagca aggcaagact gctcttgatt attggcacca gtataaagaa aataagaaaa
      421 aatgtggtag aaaagtcatt caattacctg ctcatgaagt agattacatt aaagagaaag
      481 tcactcttgg ttggacgcct gacgtc
//
LOCUS       DACEEZ010000227          503 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20313_2017
            SAMN12639966-rid9879223.denovo.239, whole genome shotgun sequence.
ACCESSION   DACEEZ010000227 DACEEZ010000000
VERSION     DACEEZ010000227.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639966
            Sequence Read Archive: SRR10023158
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 503)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 503)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741683.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741683.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:44:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,016
            CDSs (total)                      :: 2,962
            Genes (coding)                    :: 2,889
            CDSs (with protein)               :: 2,889
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 36 of 73
            Pseudo Genes (incomplete)         :: 29 of 73
            Pseudo Genes (internal stop)      :: 18 of 73
            Pseudo Genes (multiple problems)  :: 8 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..503
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639966-rid9879223.denovo.239"
                     /isolate="E20313_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            10..375
                     /locus_tag="IWO89_002953"
     CDS             10..375
                     /locus_tag="IWO89_002953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAQ0457626.1"
                     /translation="MVFDIDNLKGFLNDTSSFGFIAKENNKIIGFAYCYTLLRPDGKT
                     MFYLHSIGMLPNYQDKGYGSKLLSFIKEYSKEIGCSEMFLITDKGNPRACHVYEKLGG
                     KNDYKDEIVYVYDYEKGDK"
BASE COUNT          201 a           35 c           86 g          181 t
ORIGIN      
        1 gatgataata tggtttttga tattgataat ttaaaaggat ttcttaatga taccagttca
       61 tttgggttta tagctaaaga aaataataaa attataggat ttgcatattg ctatacactt
      121 ttaagacctg atggaaaaac aatgttttat ttacactcaa taggaatgtt acctaactat
      181 caagacaaag gttatggttc aaaattatta tcttttatta aggaatattc taaagagatt
      241 ggttgttctg aaatgttttt aataactgat aaaggtaatc ctagagcttg ccatgtatat
      301 gaaaaattag gtggtaaaaa tgattataaa gatgaaatag tatatgtata tgattatgaa
      361 aaaggtgata aataaatgaa tatagttgaa aatgaaatat gtataagaac tttaatagat
      421 gatgattttc ctttgatgtt aaaatggtta actgatgaaa gagtattaga attttatgat
      481 ggtagagata aaaaatatac att
//
LOCUS       DACEEZ010000228          502 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20313_2017
            SAMN12639966-rid9879223.denovo.240, whole genome shotgun sequence.
ACCESSION   DACEEZ010000228 DACEEZ010000000
VERSION     DACEEZ010000228.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639966
            Sequence Read Archive: SRR10023158
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 502)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 502)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741683.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741683.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:44:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,016
            CDSs (total)                      :: 2,962
            Genes (coding)                    :: 2,889
            CDSs (with protein)               :: 2,889
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 36 of 73
            Pseudo Genes (incomplete)         :: 29 of 73
            Pseudo Genes (internal stop)      :: 18 of 73
            Pseudo Genes (multiple problems)  :: 8 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..502
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639966-rid9879223.denovo.240"
                     /isolate="E20313_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            68..141
                     /locus_tag="IWO89_002954"
     tRNA            68..141
                     /locus_tag="IWO89_002954"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:102..104,aa:Ile,seq:cat)
BASE COUNT          153 a           77 c           98 g          174 t
ORIGIN      
        1 gcgataacag gtttgttgcc acacattcta ctggttgatt gtttatggct ttatgtggca
       61 acaaccagta cccttagctc agttggtcag agcagacggc tcataaccgt ccggtcgtag
      121 gttcgagtcc tacagggtac attaacgtag ccatttgaat cgttctgtgt tagaattttt
      181 tgaagagtat tatacaagct aaagcttttc ttcattgcca ctcaaatgag tggctttttt
      241 atgtatcctt ttatggatta atgaaaggat gtttcacata gttatatttc tgtatatttg
      301 aaaagtttta ctttgatttt taaatagaaa gacatttggg ttatattgtg agataataat
      361 aaagaagagt ttaaagcgtt ccccaaaaac cactgcccca taagtgtgtt acgctttaaa
      421 ctcttttata tttgaagcca ttaaaaagca taccatattt ttgaaaaaaa gtgagaaaaa
      481 aggcttataa ttggagtggt ag
//
LOCUS       DACEEZ010000229          487 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20313_2017
            SAMN12639966-rid9879223.denovo.241, whole genome shotgun sequence.
ACCESSION   DACEEZ010000229 DACEEZ010000000
VERSION     DACEEZ010000229.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639966
            Sequence Read Archive: SRR10023158
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 487)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 487)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741683.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741683.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:44:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,016
            CDSs (total)                      :: 2,962
            Genes (coding)                    :: 2,889
            CDSs (with protein)               :: 2,889
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 36 of 73
            Pseudo Genes (incomplete)         :: 29 of 73
            Pseudo Genes (internal stop)      :: 18 of 73
            Pseudo Genes (multiple problems)  :: 8 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..487
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639966-rid9879223.denovo.241"
                     /isolate="E20313_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(<1..>487)
                     /locus_tag="IWO89_002955"
     CDS             complement(<1..>487)
                     /locus_tag="IWO89_002955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DDE-type integrase/transposase/recombinase"
                     /protein_id="HAQ0457627.1"
                     /translation="IHRRFYTSICHQKITTDTTEFKYHEVDAKGIIRQKKLYLDPFMD
                     MFNSEILSYRISEKPNALAVMEGLEEAIQTTNDCPYRRTFHSDQGWAYQMKAYRNKLK
                     EYKIFQSMSRKGNCLDNSLMENFFGLLKQEIFHGKVYNCFEELKSAIDSYIYYYNNER
                     IK"
BASE COUNT          142 a           92 c           86 g          167 t
ORIGIN      
        1 gttttatccg ttcattattg tagtaataga tatagctatc gatggctgat tttaattcct
       61 cgaaacagtt ataaactttc ccgtgaaaaa tctcttgttt taataagcca aaaaaattct
      121 ccataagtga attatctaag cagttccctt tccgagacat gctttgaaag attttgtact
      181 ccttcaattt gtttctataa gctttcattt gatacgccca accttgatct gagtgaaacg
      241 tacgacgata gggacaatcg ttagtggtct ggatagcttc ctccaaacct tccattaccg
      301 ctaatgcatt gggcttttca gagatacgat aggataaaat ctcactattg aacatatcca
      361 taaaaggatc tagatacaat ttcttttgac ggataatgcc tttggcatcc acttcatggt
      421 atttaaattc agttgtatcc gtcgtgattt tctgatggca gatactggtg tagaaacgac
      481 gatggat
//
LOCUS       DACEEZ010000230          484 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20313_2017
            SAMN12639966-rid9879223.denovo.242, whole genome shotgun sequence.
ACCESSION   DACEEZ010000230 DACEEZ010000000
VERSION     DACEEZ010000230.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639966
            Sequence Read Archive: SRR10023158
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 484)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 484)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741683.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741683.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:44:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,016
            CDSs (total)                      :: 2,962
            Genes (coding)                    :: 2,889
            CDSs (with protein)               :: 2,889
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 36 of 73
            Pseudo Genes (incomplete)         :: 29 of 73
            Pseudo Genes (internal stop)      :: 18 of 73
            Pseudo Genes (multiple problems)  :: 8 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..484
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639966-rid9879223.denovo.242"
                     /isolate="E20313_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(61..258)
                     /locus_tag="IWO89_002956"
     CDS             complement(61..258)
                     /locus_tag="IWO89_002956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HAQ0457628.1"
                     /translation="MNNGTVKWFNSDKGFGFITGEDGNDVFAHFSAIQGEGFKSLDEG
                     QAVTYDIEEGQRGPQAVNIVK"
BASE COUNT          158 a           89 c           67 g          170 t
ORIGIN      
        1 aattttttct accgacataa acaaaaaaac cacctcatat gaggcggtta acgttcaaca
       61 ttattttacg atatttactg cttgaggacc acgttgtcct tcttcaatat cataagtaac
      121 tgcttggcct tcatctaaag acttaaagcc ttctccttgg attgctgaga aatgagcgaa
      181 tacgtcattt ccatcttcac cagtgatgaa accaaaacct ttatctgagt taaaccattt
      241 tactgtaccg ttattcatat atatttcctc ctgatacgta tataatacgt gttttattgc
      301 aattaatatt acttggtaaa aggagttttt atagtgtgaa tatatcgctc agattacgtt
      361 tcaaattaga ttacttattt actataacac ggatgtaatg ctaacacaag gataatcact
      421 cgatatacgg tttaaatatt attatttgtc aaattaagcg agacaaaact ttccatttac
      481 gtaa
//
LOCUS       DACEEZ010000231          482 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20313_2017
            SAMN12639966-rid9879223.denovo.243, whole genome shotgun sequence.
ACCESSION   DACEEZ010000231 DACEEZ010000000
VERSION     DACEEZ010000231.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639966
            Sequence Read Archive: SRR10023158
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 482)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 482)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741683.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741683.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:44:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,016
            CDSs (total)                      :: 2,962
            Genes (coding)                    :: 2,889
            CDSs (with protein)               :: 2,889
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 36 of 73
            Pseudo Genes (incomplete)         :: 29 of 73
            Pseudo Genes (internal stop)      :: 18 of 73
            Pseudo Genes (multiple problems)  :: 8 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..482
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639966-rid9879223.denovo.243"
                     /isolate="E20313_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            1..482
                     /locus_tag="IWO89_002957"
                     /pseudo
     CDS             1..482
                     /locus_tag="IWO89_002957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558187.1"
                     /note="frameshifted; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3-like element IS1485 family transposase"
BASE COUNT          201 a           82 c           88 g          111 t
ORIGIN      
        1 gtaaagcaat ctaaaacgag tggttcattc aaagaaaaag ataatcttac gcctgaacaa
       61 aaagaattgt tagaactacg taaaagaaac cagcaattag aaatggaaaa tgatatttta
      121 aagcaagcag cgctgatatt cggacgaaga gacaagtaat cgatgcgaat aagcatcttt
      181 accctatatc agcgatgtgc agaatattag gtctatcacg tcagtcctat tattatcaat
      241 caaaaccaaa gaaagacgaa tcagaacttg aagaagtagt cgctgaagaa tttatccgca
      301 gccgaaaggc ctacggctca agaaaaataa aaaaagcctt atcaaaacga ggcattcaga
      361 tcagccgacg aaaaattagt agaatcatga aaaatagagg attaaaatcg agctatactg
      421 ttgcttattt taaagtacat cattctactt gcaatgaagc caaaacgaca aacgtattga
      481 at
//
LOCUS       DACEEZ010000232          471 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20313_2017
            SAMN12639966-rid9879223.denovo.244, whole genome shotgun sequence.
ACCESSION   DACEEZ010000232 DACEEZ010000000
VERSION     DACEEZ010000232.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639966
            Sequence Read Archive: SRR10023158
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 471)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 471)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741683.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741683.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:44:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,016
            CDSs (total)                      :: 2,962
            Genes (coding)                    :: 2,889
            CDSs (with protein)               :: 2,889
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 36 of 73
            Pseudo Genes (incomplete)         :: 29 of 73
            Pseudo Genes (internal stop)      :: 18 of 73
            Pseudo Genes (multiple problems)  :: 8 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..471
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639966-rid9879223.denovo.244"
                     /isolate="E20313_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            <1..>471
                     /locus_tag="IWO89_002958"
     CDS             <1..>471
                     /locus_tag="IWO89_002958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HAQ0457629.1"
                     /translation="KIPHFSTFGKNYVRRFRETTVFEDIFAYILEQAVKAGFVTEDNL
                     YLDSTHIKANANKHKFTKEMTHDEAKAYQDELEDEINRQRIEAGKRPFTLDLEKEVKL
                     KERKISKADPESGYYVKGEREKQFAYSAHTSCDDNGFILSTIITPGNIHDSQVAF"
BASE COUNT          169 a           86 c           94 g          122 t
ORIGIN      
        1 aaaatccccc atttctcaac ttttggaaag aattatgttc gccgatttag agaaacgacg
       61 gtgtttgagg atatttttgc ctatatttta gaacaagcag ttaaagcagg ttttgtaaca
      121 gaggataatt tgtatctgga ttctacccac atcaaagcta acgccaataa acataagttt
      181 acgaaagaaa tgacccatga tgaagcgaaa gcctatcagg atgaattaga agatgagatc
      241 aatcgtcaac gaatagaggc tggaaaacgc ccttttactt tggacttaga aaaggaagtg
      301 aagctgaaag aaagaaaaat aagtaaagct gatccggaaa gcggctacta cgtcaaaggc
      361 gaacgagaaa aacaatttgc ttattctgcc catacttcat gcgacgacaa tggatttatt
      421 cttagcacaa tcatcactcc tggaaatatt catgacagtc aagtcgcctt t
//
LOCUS       DACEEZ010000233          453 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20313_2017
            SAMN12639966-rid9879223.denovo.245, whole genome shotgun sequence.
ACCESSION   DACEEZ010000233 DACEEZ010000000
VERSION     DACEEZ010000233.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639966
            Sequence Read Archive: SRR10023158
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 453)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 453)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741683.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741683.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:44:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,016
            CDSs (total)                      :: 2,962
            Genes (coding)                    :: 2,889
            CDSs (with protein)               :: 2,889
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 36 of 73
            Pseudo Genes (incomplete)         :: 29 of 73
            Pseudo Genes (internal stop)      :: 18 of 73
            Pseudo Genes (multiple problems)  :: 8 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..453
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639966-rid9879223.denovo.245"
                     /isolate="E20313_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            <1..>453
                     /locus_tag="IWO89_002959"
     CDS             <1..>453
                     /locus_tag="IWO89_002959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0457630.1"
                     /translation="IESGTTVFAVKDGVSLPEDKLPVLKAKDGYTDAKWPEEATQPIT
                     ADDTEFVSSATKLDDKSDADKYNPEGQKVTTELNKEPDAFEGIKNKEDLPKDTKYTWK
                     EKVDVSAAGNKKGTVVVTYPDGSSDEVEVDVTVTDNRSDADKYEPTVEG"
BASE COUNT          183 a           67 c          105 g           98 t
ORIGIN      
        1 tattgaaagt ggaacaacag tctttgcagt gaaagacggc gtaagcttac cagaagataa
       61 acttccagtg ttgaaagcaa aagatggtta tacagatgcg aaatggccag aggaagcaac
      121 tcaaccaatt acagcagatg atacagaatt tgtatcaagt gcaacaaaac tggatgataa
      181 atctgacgct gacaaatata atcctgaagg tcaaaaagtg actacagaat tgaataagga
      241 accagacgca tttgagggaa ttaagaacaa agaagattta ccaaaagata ctaagtatac
      301 ttggaaagaa aaagtagatg ttagcgcagc tggaaataaa aaagggactg ttgtagtgac
      361 atatccagat ggatcatctg atgaagttga agtagatgtt acagtgacag acaatcgttc
      421 tgacgccgat aaatatgagc caacagtaga agg
//
LOCUS       DACEEZ010000234          437 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20313_2017
            SAMN12639966-rid9879223.denovo.246, whole genome shotgun sequence.
ACCESSION   DACEEZ010000234 DACEEZ010000000
VERSION     DACEEZ010000234.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639966
            Sequence Read Archive: SRR10023158
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 437)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 437)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741683.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741683.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:44:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,016
            CDSs (total)                      :: 2,962
            Genes (coding)                    :: 2,889
            CDSs (with protein)               :: 2,889
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 36 of 73
            Pseudo Genes (incomplete)         :: 29 of 73
            Pseudo Genes (internal stop)      :: 18 of 73
            Pseudo Genes (multiple problems)  :: 8 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..437
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639966-rid9879223.denovo.246"
                     /isolate="E20313_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
BASE COUNT          182 a           61 c           73 g          121 t
ORIGIN      
        1 cattattatc aaaataatgt taaaaataaa aaagaattgt ccatcactgt tattcaaatc
       61 attttatcaa aactacatag taaatgttaa aaagcaacat gacataatat atagaaggct
      121 ttacagtata tatgggagct tactaaaaaa gaatggagta agagttaaca caatgggcca
      181 ttagctgaag ttttaagcac ttcaaggtat gaaacagaat ggcctgtaat actacatgat
      241 tatttagact gctcaaactg ctatcagtct ggtggaatga ataaagtatt atactaaaag
      301 cagatccaac tacaacgtaa caggaaacca gttgtatcag gaaaagtgat agtacatgga
      361 gaagaaaagg ccccctttgt atgagtcagt acatatctta tttatagata aaacggtatt
      421 caatcgaaat aaaaaat
//
LOCUS       DACEEZ010000235          375 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20313_2017
            SAMN12639966-rid9879223.denovo.247, whole genome shotgun sequence.
ACCESSION   DACEEZ010000235 DACEEZ010000000
VERSION     DACEEZ010000235.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639966
            Sequence Read Archive: SRR10023158
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 375)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 375)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741683.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741683.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:44:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,016
            CDSs (total)                      :: 2,962
            Genes (coding)                    :: 2,889
            CDSs (with protein)               :: 2,889
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 36 of 73
            Pseudo Genes (incomplete)         :: 29 of 73
            Pseudo Genes (internal stop)      :: 18 of 73
            Pseudo Genes (multiple problems)  :: 8 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..375
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639966-rid9879223.denovo.247"
                     /isolate="E20313_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(<1..132)
                     /locus_tag="IWO89_002960"
     CDS             complement(<1..132)
                     /locus_tag="IWO89_002960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014748673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HAQ0457631.1"
                     /translation="MKLFVGIDVSSEKLDVCFLTDDNQLSILSEISVANDIEGASFIR
                     "
BASE COUNT          132 a           69 c           76 g           98 t
ORIGIN      
        1 tcgtatgaat gaagcacctt cgatgtcatt ggcaacagag atttcagata agatagacaa
       61 ttgattgtcg tctgttaaaa aacaaacatc tagtttttca gagctaacgt caataccgac
      121 aaataatttc atggaagaag gacctccttt cgatataaat tttggaaaat gcttcgatac
      181 tgtgggattt cccagaaact cactgtatag ccacaacctc gcctaataga atacggtgat
      241 aaaagagcta gaagctgcct tccaaactac tatttgaaag tctagtccat tatcgaaaca
      301 gctaatgagt tggtagtgat aacagtggtt cgggtaacag actaaacagg gtagacaaac
      361 gctacaggag gaaat
//
LOCUS       DACEEZ010000236          347 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20313_2017
            SAMN12639966-rid9879223.denovo.248, whole genome shotgun sequence.
ACCESSION   DACEEZ010000236 DACEEZ010000000
VERSION     DACEEZ010000236.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639966
            Sequence Read Archive: SRR10023158
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 347)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 347)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741683.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741683.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:44:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,016
            CDSs (total)                      :: 2,962
            Genes (coding)                    :: 2,889
            CDSs (with protein)               :: 2,889
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 36 of 73
            Pseudo Genes (incomplete)         :: 29 of 73
            Pseudo Genes (internal stop)      :: 18 of 73
            Pseudo Genes (multiple problems)  :: 8 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..347
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639966-rid9879223.denovo.248"
                     /isolate="E20313_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(<1..321)
                     /locus_tag="IWO89_002961"
     CDS             complement(<1..321)
                     /locus_tag="IWO89_002961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000997695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="HAQ0457632.1"
                     /translation="MNDFTTEIVQTLVTKGDLNELFRSHLEKAINTLLRTELTAFLDY
                     EKYDRTGFNSGNSRNGSYFRSIKTEYGELTLEIPRDRNGEFKQQTLPAYKRTNDTLET
                     TIIHL"
BASE COUNT           97 a           55 c           64 g          131 t
ORIGIN      
        1 taaatggata atagtggttt ccaatgtatc gtttgttctt ttgtaggctg gtaaagtttg
       61 ttgtttaaac tcaccattac gatctctagg tatttccaat gttaattcac catattcggt
      121 tttgattgat cgaaagtaag aaccgtttct cgaattacct gaattaaaac cagtgcgatc
      181 atatttttcg taatctaaaa aagccgttaa ttcagtccgt aggagtgtgt ttatcgcttt
      241 ttctaagtgc gaacggaata attcatttaa atcgccttta gtgactagag tttgcacaat
      301 ttctgtagta aaatcattca tagggaagtc ctcttttctg tgaattg
//
LOCUS       DACEEZ010000237          344 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20313_2017
            SAMN12639966-rid9879223.denovo.249, whole genome shotgun sequence.
ACCESSION   DACEEZ010000237 DACEEZ010000000
VERSION     DACEEZ010000237.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639966
            Sequence Read Archive: SRR10023158
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 344)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 344)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741683.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741683.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:44:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,016
            CDSs (total)                      :: 2,962
            Genes (coding)                    :: 2,889
            CDSs (with protein)               :: 2,889
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 36 of 73
            Pseudo Genes (incomplete)         :: 29 of 73
            Pseudo Genes (internal stop)      :: 18 of 73
            Pseudo Genes (multiple problems)  :: 8 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..344
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639966-rid9879223.denovo.249"
                     /isolate="E20313_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            63..>344
                     /locus_tag="IWO89_002962"
     CDS             63..>344
                     /locus_tag="IWO89_002962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ0457633.1"
                     /translation="METTDTLLQLLQEAHKTNQAQQQTIQNLTTEIQLLNEKVNYLTN
                     KLFGRSKETLFEETNGQLNLFSDEEISVSVPEAAATIIPVKGHQRVVGTK"
BASE COUNT          123 a           62 c           63 g           96 t
ORIGIN      
        1 tcttgtgcgt ttcttgtggt attctgtaac aaaatcagtt cgattcaagg tggtgaaaaa
       61 ttttggaaac tacagatacg ttgcttcagt tgcttcaaga agcccataaa accaatcaag
      121 cacaacaaca aaccattcag aatcttacaa ctgaaatcca actattgaat gaaaaggtca
      181 attatttaac caacaaatta tttggacgct ctaaagagac tctctttgag gaaacgaatg
      241 ggcaattgaa tctttttagt gatgaagaaa tttctgtaag tgttccagaa gcagctgcca
      301 ccattattcc agtaaaagga catcaacgag ttgtaggaac gaaa
//
LOCUS       DACEEZ010000238          321 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20313_2017
            SAMN12639966-rid9879223.denovo.250, whole genome shotgun sequence.
ACCESSION   DACEEZ010000238 DACEEZ010000000
VERSION     DACEEZ010000238.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639966
            Sequence Read Archive: SRR10023158
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 321)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 321)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741683.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741683.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:44:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,016
            CDSs (total)                      :: 2,962
            Genes (coding)                    :: 2,889
            CDSs (with protein)               :: 2,889
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 36 of 73
            Pseudo Genes (incomplete)         :: 29 of 73
            Pseudo Genes (internal stop)      :: 18 of 73
            Pseudo Genes (multiple problems)  :: 8 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..321
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639966-rid9879223.denovo.250"
                     /isolate="E20313_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(<1..>321)
                     /locus_tag="IWO89_002963"
     CDS             complement(<1..>321)
                     /locus_tag="IWO89_002963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HAQ0457634.1"
                     /translation="ITRHVWGDLMDEVEHLRLTDLNKSIYKKRKQTIERIFADAKEKH
                     GMRWTKYRGLEKVATHTMLVFAAMNLKKLATWLWKGKEPLFFCSKIRNEVDKKLFQAR
                     VTSL"
BASE COUNT           79 a           78 c           51 g          113 t
ORIGIN      
        1 tccaaactcg taactcgagc ttggaacagc tttttatcta cttcgtttcg aatttttgaa
       61 caaaaaaata aaggctcctt gcctttccat agccaggtgg ctaatttctt taaattcatt
      121 gcagcaaaca ctagcatcgt atgcgtcgcg actttttcca agcctcgata cttggtccaa
      181 cgcatcccat gcttttcttt tgcatccgca aatattcgtt caatcgtttg tttccgtttt
      241 ttgtaaattg atttattcag atctgttagg cgaagatgtt ccacttcatc catcagatct
      301 ccccaaacat gtctcgtgat g
//
LOCUS       DACEEZ010000239          318 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20313_2017
            SAMN12639966-rid9879223.denovo.251, whole genome shotgun sequence.
ACCESSION   DACEEZ010000239 DACEEZ010000000
VERSION     DACEEZ010000239.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639966
            Sequence Read Archive: SRR10023158
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 318)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 318)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741683.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741683.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:44:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,016
            CDSs (total)                      :: 2,962
            Genes (coding)                    :: 2,889
            CDSs (with protein)               :: 2,889
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 36 of 73
            Pseudo Genes (incomplete)         :: 29 of 73
            Pseudo Genes (internal stop)      :: 18 of 73
            Pseudo Genes (multiple problems)  :: 8 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..318
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639966-rid9879223.denovo.251"
                     /isolate="E20313_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
BASE COUNT          110 a           54 c           68 g           86 t
ORIGIN      
        1 tttatcgaga ggttgtgaaa tcagcgaatt aacctgagta ttagagaaaa aaatggaaaa
       61 atagtccctc atattttacc gtttgttgaa ctaataccga gggtcagctg attgaacacc
      121 gtttagtgga gaggtcgtaa gcctaccccc cagctcctag ttacaaggac aaattcaaaa
      181 aacagctctt catattttat gaatgttgta cttgtgattc ggaggtggca agggaacact
      241 gtttattcaa aaagaggctg ggacagaagc gtttaactcc aagaaataag aagaaattca
      301 cgaaaattgc ttttcaaa
//
LOCUS       DACEEZ010000240          317 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20313_2017
            SAMN12639966-rid9879223.denovo.252, whole genome shotgun sequence.
ACCESSION   DACEEZ010000240 DACEEZ010000000
VERSION     DACEEZ010000240.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639966
            Sequence Read Archive: SRR10023158
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 317)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 317)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741683.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741683.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:44:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,016
            CDSs (total)                      :: 2,962
            Genes (coding)                    :: 2,889
            CDSs (with protein)               :: 2,889
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 36 of 73
            Pseudo Genes (incomplete)         :: 29 of 73
            Pseudo Genes (internal stop)      :: 18 of 73
            Pseudo Genes (multiple problems)  :: 8 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..317
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639966-rid9879223.denovo.252"
                     /isolate="E20313_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            <1..>317
                     /locus_tag="IWO89_002964"
     CDS             <1..>317
                     /locus_tag="IWO89_002964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HAQ0457635.1"
                     /translation="RQAKLLNQIAKVGYNSTKKSYFYGLKIHMIVTKTGFPITYSITN
                     PGVHDVKVLETLSEEANLPNILGDKGYISHKIHEKLALKGITISVPPRKNMDKSEKLD
                     HSL"
BASE COUNT          117 a           64 c           52 g           84 t
ORIGIN      
        1 tagacaagca aaactattga atcaaatagc caaagttggc tataactcaa caaaaaaatc
       61 ttatttttat ggtcttaaaa ttcatatgat cgttactaag actggctttc cgattactta
      121 ctcaatcacg aatccaggtg tccatgacgt gaaagtgtta gagactttat cggaagaagc
      181 gaatcttccc aatatcctag gggacaaagg atatattagc cataaaatcc atgaaaagct
      241 tgcccttaag ggcattacta tatccgttcc gccacgtaaa aacatggata aatcagaaaa
      301 actagaccac agcttac
//
LOCUS       DACEEZ010000241          313 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20313_2017
            SAMN12639966-rid9879223.denovo.253, whole genome shotgun sequence.
ACCESSION   DACEEZ010000241 DACEEZ010000000
VERSION     DACEEZ010000241.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639966
            Sequence Read Archive: SRR10023158
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 313)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 313)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741683.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741683.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:44:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,016
            CDSs (total)                      :: 2,962
            Genes (coding)                    :: 2,889
            CDSs (with protein)               :: 2,889
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 36 of 73
            Pseudo Genes (incomplete)         :: 29 of 73
            Pseudo Genes (internal stop)      :: 18 of 73
            Pseudo Genes (multiple problems)  :: 8 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..313
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639966-rid9879223.denovo.253"
                     /isolate="E20313_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            <1..294
                     /locus_tag="IWO89_002965"
     CDS             <1..294
                     /locus_tag="IWO89_002965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HAQ0457636.1"
                     /translation="SSTTSDSSDSSTSSDSTDTSSTTSDTRHASVSFSKTSSDNRTNF
                     NLTNKTSNDNGNKSKRALPKTGSQSNNWITLAGVILLVIGLRITFMSYKKSKR"
BASE COUNT          127 a           61 c           49 g           76 t
ORIGIN      
        1 tcctctacca caagtgatag ctcagattca tcaacttcaa gcgatagtac agacacgtcg
       61 tctaccacaa gcgatacacg tcatgcaagt gtctccttct caaaaacaag ttcggataac
      121 aggacgaact ttaacttaac taataaaaca agcaatgaca atggaaacaa atcaaaaaga
      181 gcacttccta aaactggaag tcaatcgaat aattggataa cactcgcagg cgttatatta
      241 cttgtaatcg gtttaagaat cacttttatg agttataaga aatcgaaaag gtaaaaaaat
      301 attcaagata aaa
//
LOCUS       DACEEZ010000242         6534 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20313_2017
            SAMN12639966-rid9879223.guided.116, whole genome shotgun sequence.
ACCESSION   DACEEZ010000242 DACEEZ010000000
VERSION     DACEEZ010000242.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639966
            Sequence Read Archive: SRR10023158
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6534)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6534)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741683.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741683.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:44:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,016
            CDSs (total)                      :: 2,962
            Genes (coding)                    :: 2,889
            CDSs (with protein)               :: 2,889
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 36 of 73
            Pseudo Genes (incomplete)         :: 29 of 73
            Pseudo Genes (internal stop)      :: 18 of 73
            Pseudo Genes (multiple problems)  :: 8 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6534
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639966-rid9879223.guided.116"
                     /isolate="E20313_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            74..190
                     /locus_tag="IWO89_002966"
     CDS             74..190
                     /locus_tag="IWO89_002966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001814923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance determinant leader
                     peptide"
                     /protein_id="HAQ0457637.1"
                     /translation="MILKYPENICMLCIPMVMHKNPSDKSIYHWDFYALLGF"
     gene            206..2125
                     /gene="tet(M)"
                     /locus_tag="IWO89_002967"
     CDS             206..2125
                     /gene="tet(M)"
                     /locus_tag="IWO89_002967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019117172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance ribosomal protection
                     protein Tet(M)"
                     /protein_id="HAQ0457638.1"
                     /translation="MKIINIGVLAHVDAGKTTLTESLLYNSGAITELGSVDKGTTRTD
                     NTLLERQRGITIQTGITSFQWENTKVNIIDTPGHMDFLAEVYRSLSVLDGAILLISAK
                     DGVQAQTRILFHALRKMGIPTIFFINKIDQNGIDLSTVYQDIKEKLSMEIIIKQKVEL
                     HPNMCVMSCTEPEQWDVVIEGNDDLLEKYMSGKSLEALELEQEEIRRFQNCSLYPVYH
                     GSAKSNIGIEQLIEVITNKFYSSTYRKKSELCGNVFKIEYSEERQRLAYVRLYSGVLH
                     LRDSVRISEKEKIKITEMYTSINGELCKIDKAYSGEIVILQNEFLKLNSVLGDTKLLP
                     QRERIENPLPLLQTTVEPSKPQQREMLLDALLEISDSDPLLQYYVDSTTHEIILSFLG
                     KVQMEVTCALLQEKYHVEVKIKKPTVIYMERPLKKAEYTIHIEVPPNPFWASIGLSVE
                     PLPLGSGVQYESSVSLGYLNQSFQNAVMEGIRYGCEQGLYGWNVTDCKICFKYGLYYS
                     PVSTPADFRMLAPIVLEQVLKKAGTELLEPYLSFKIYAPQEYLSRAYNDAPKYCANIV
                     DTQLKNNEVILSGEIPARCIQEYRSDLTFFTNGRSVCLTELKGYHVTTGEPVCQPRRP
                     NSRIDKVRYMFNKIT"
     gene            2235..2285
                     /locus_tag="IWO89_002968"
                     /pseudo
     CDS             2235..2285
                     /locus_tag="IWO89_002968"
                     /inference="COORDINATES: protein motif:HMM:NF019662.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance efflux system leader
                     peptide"
     gene            2319..3695
                     /gene="tet(L)"
                     /locus_tag="IWO89_002969"
     CDS             2319..3695
                     /gene="tet(L)"
                     /locus_tag="IWO89_002969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001574277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline efflux MFS transporter Tet(L)"
                     /protein_id="HAQ0457639.1"
                     /translation="MNTSYSQSNLRHNQILIWLCILSFFSVLNEMVLNVSLPDIANDF
                     NKSPASTNWVNTAFMLTFSIGTAVYGKLSDQLGIKRLLLFGIIINCFGALIGFVGHSF
                     FSLLIMARFIQGAGAAAFPALVMVVVARYIPKENRGKAFGLIGSIVAMGEGVGPAIGG
                     MIAHYIHWSYLLLIPTITIITVPFLMKLLKKEVRIKGHFDIKGIILMSVGIVFFMLFT
                     TSYSISFLIVSVLSFLIFVKHIRKVTDPFVDPGLGKNIPFMIGVLCGGIIFGTVAGFV
                     SMVPYMMKDVYQLSTAEIGSVIIFPGTMSVIIFGYIGGILVDRRGPLYVLNIGVTFLS
                     VSFLTASFLLETTSWFMTIIMVFVLGGLSFTKTVISTIVSSSLKQQEAGAGMSLLNFT
                     SFLSEGTGIAIVGGLLSIPLLDQRLLPMEVDQSTYLYSNLLLLFSGIIVISWLVTLNV
                     YKHSQRDF"
     gene            4259..5521
                     /locus_tag="IWO89_002970"
     CDS             4259..5521
                     /locus_tag="IWO89_002970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001619005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Plasmid recombination enzyme type 2"
                     /protein_id="HAQ0457640.1"
                     /translation="MSYAVCRMQKVKSAGLKGMQFHNQRERKSRTNDDIDHERTRENY
                     DLKNDKNIDYNERVKEIIESQKTGTRKTRKDAVLVNELLVTSDRDFFEQLDPGEQKRF
                     FEESYKLFSERYGKQNIAYATVHNDEQTPHMHLGVVPMRDGKLQGKNVFNRQELLWLQ
                     DKFPEHMKKQGFELKRGERGSDRKHIETAKFKKQTLEKEIDFLEKNLAVKKDEWTAYS
                     DKVKSDLEVPAKRHMKSVEVPTGEKSMFGLGKEIMKTEKKPTKNVVISERDYKNLVTA
                     ARDNDRLKQHVRNLMSTDMAREYKKLSKEHGQVKEKYSGLVERFNENVNDYNELLEEN
                     KSLKSKISDLKRDVSLIYESTKEFLKERTDGLKAFKNVFKGFVDKVKDKTAQFQEKHD
                     LEPKKNEFELTHNREVKKERSRDQGMSL"
     gene            5766..6518
                     /locus_tag="IWO89_002971"
     CDS             5766..6518
                     /locus_tag="IWO89_002971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001611915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein rep"
                     /protein_id="HAQ0457641.1"
                     /translation="MCPNSSIYSDEKSRVLVDKTKSGKVRPWREKKIANVDYFELLHI
                     LEFKKAERVKDCAEILEYKQNRETGERKLYRVWFCKSRLCPMCNWRRAMKHGIQSQKV
                     VAEVIKQKPTVRWLFLTLTVKNVYDGEELNKSLSDMAQGFRRMMQYKKINKNLVGFMR
                     ATEVTINNKDNSYNQHMHVLVCVEPTYFKNTENYVNQKQWIQFWKKAMKLDYDPNVKV
                     QMIRPKNKYKSDIQSAIDETAKYPVKGSVAKF"
BASE COUNT         2257 a          937 c         1338 g         2002 t
ORIGIN      
        1 ggaacaaata attagatgtc cttttttagg agggcttagt tttttgtacc cagtttaaga
       61 atacctttat catgtgattc taaagtatcc agagaatatc tgtatgcttt gtatacctat
      121 ggttatgcat aaaaatccca gtgataaaag tatttatcac tgggattttt atgccctttt
      181 gggtttttga atggaggaaa atcacatgaa aattattaat attggagttt tagctcatgt
      241 tgatgcagga aaaactacct taacagaaag cttattatat aacagtggag cgattacaga
      301 attaggaagc gtggacaaag gtacaacgag gacggataat acgcttttag aacgtcagag
      361 aggaattaca attcagacag gaataacctc ttttcagtgg gaaaatacga aggtgaacat
      421 catagacacg ccaggacata tggatttctt agcagaagta tatcgttcat tatcagtttt
      481 agatggggca attctactga tttctgcaaa agatggcgta caagcacaaa ctcgtatatt
      541 atttcatgca cttaggaaaa tggggattcc cacaatcttt tttatcaata agattgacca
      601 aaatggaatt gatttatcaa cggtttatca ggatattaaa gagaaacttt ctatggaaat
      661 tataatcaaa cagaaagtag agctgcaccc taatatgtgt gtgatgagct gtacggaacc
      721 tgagcaatgg gatgtggtaa tagaaggaaa tgatgacctt ttagagaaat atatgtccgg
      781 taaatcatta gaagcattag aactcgaaca agaggaaatc agaagatttc agaattgctc
      841 cttgtaccct gtttatcatg gaagcgcaaa aagcaacata gggattgagc agcttataga
      901 agtgataacg aataaatttt attcatcaac atacagaaag aagtctgaac tttgcggaaa
      961 tgtcttcaaa attgaatatt cggaagaaag acaacgtctt gcatatgtac gcctttatag
     1021 tggagtacta catttgcggg attcggttag aatatcggaa aaggaaaaaa taaaaattac
     1081 agaaatgtat acttcaataa atggtgaatt atgtaaaatt gataaggctt attccgggga
     1141 aattgttatt ttgcaaaatg agtttttgaa gctaaatagt gttcttggag atacaaagct
     1201 attgccacag agagagagaa ttgaaaatcc gctccctctg ctgcaaacaa ctgttgaacc
     1261 gagcaaacct caacaaaggg aaatgttact tgatgcactt ttagaaatct ccgacagtga
     1321 cccgcttcta caatattatg tggattctac gacacatgaa atcatacttt ctttcttagg
     1381 gaaagtacaa atggaagtga cttgtgctct attgcaagaa aagtatcatg tggaggtaaa
     1441 aataaaaaag cctacagtca tttatatgga aagaccgtta aaaaaagcag agtataccat
     1501 tcacatcgaa gtgccaccga atcccttctg ggcttccatt ggtctttctg tagaaccgct
     1561 tccattaggg agcggagtac agtatgagag ctcggtttct cttggatact taaatcaatc
     1621 gtttcaaaat gcagttatgg aagggatacg atatggctgt gaacaaggat tgtatggttg
     1681 gaatgtgacg gactgtaaaa tctgttttaa gtatggctta tactatagcc ctgttagtac
     1741 cccagcagat tttcggatgc ttgctcctat tgtattggaa caagtcttaa aaaaagctgg
     1801 aacagaattg ttagagccat atcttagttt taaaatttat gcgccacagg aatatctttc
     1861 acgagcatac aacgatgctc ctaaatattg tgcgaacatc gtagacactc aattgaaaaa
     1921 taatgaggtc attcttagtg gagaaatccc tgctcggtgt attcaagaat atcgtagtga
     1981 tttaactttc tttacaaatg gacgtagtgt ttgtttaaca gagttaaaag ggtaccatgt
     2041 tactaccggt gaacctgttt gccagccccg tcgtccaaat agtcggatag ataaagtacg
     2101 atatatgttc aataaaataa cttagtgtat tttatgttgt tatataaata tggtttcttg
     2161 ttaaataaga tgaaatattt tttaataaag atttgaatta aagtgtaaag gaggaattaa
     2221 ttatgaagtg aaatgaatgt aacagggttc aattaaaaga gggaagcgta tcattaaccc
     2281 tataaactac gtctgccctc attattggag ggtgaaatgt gaatacatcc tattcacaat
     2341 cgaatttacg acacaaccaa attttaattt ggctttgcat tttatctttt tttagcgtat
     2401 taaatgaaat ggttttgaac gtctcattac ctgatattgc aaatgatttt aataaatcac
     2461 ctgcgagtac aaactgggtg aacacagcct ttatgttaac cttttccatt ggaacagctg
     2521 tatatggaaa gctatctgat caattaggca tcaaaaggtt actcctattt ggaattataa
     2581 taaattgttt cggggcgtta attgggtttg ttggccattc tttcttttcc ttacttatta
     2641 tggctcgttt tattcaaggg gctggtgcag ctgcatttcc agcactcgta atggttgtag
     2701 ttgcgcgcta tattccaaag gaaaataggg gtaaagcatt tggtcttatt ggatcgatag
     2761 tagccatggg agaaggagtc ggtccagcga ttggtggaat gatagcccat tatattcatt
     2821 ggtcctatct tctactcatt cctacgataa caattatcac tgttccgttt cttatgaaat
     2881 tattaaagaa agaagtaagg ataaaaggtc attttgatat caaaggaatt atactaatgt
     2941 ctgtaggcat tgtatttttt atgttgttta caacatcata tagcatttct tttcttatcg
     3001 ttagcgtgct gtcattcctg atatttgtaa aacatatcag gaaagtaaca gatccttttg
     3061 ttgatcccgg attagggaaa aatatacctt ttatgattgg agttctttgt gggggaatta
     3121 tatttggaac agtagcaggg tttgtctcta tggttcctta tatgatgaaa gatgtttacc
     3181 agctaagtac tgccgaaatc ggaagtgtaa ttattttccc tggaacaatg agtgtcatta
     3241 ttttcggcta cattggtggg atacttgttg atagaagagg tcctttatac gtgttaaaca
     3301 tcggagttac atttctttct gttagctttt taactgcttc ctttctttta gaaacaacat
     3361 catggttcat gacaattata atggtatttg ttttaggtgg gctttcgttc accaaaacag
     3421 ttatatcaac aattgtttca agtagcttga aacagcagga agctggtgct ggaatgagtt
     3481 tgcttaactt taccagcttt ttatcagagg gaacaggtat tgcaattgta ggtggtttat
     3541 tatccatacc cttacttgat caaaggttgt tacctatgga agttgatcag tcaacttatc
     3601 tgtatagtaa tttgttatta cttttttcag gaatcattgt cattagttgg ctggttacct
     3661 tgaatgtata taaacattct caaagggatt tctaaatcgt taagggatca actttgggag
     3721 agagttcaaa attgatcctt tttttataac aggaattcaa atctttttgt tccattaaag
     3781 ggcgcgattg ctgaataaaa gatacgagag acctctcttg tatctttttt attttgagtg
     3841 gttttgtccg ttacactaga aaaccgaaag acaataaaaa ttttattctt gctgagtctg
     3901 gctttcggta agctagacaa aacggacaaa ataaaaattg gcaagggttt aaaggtggag
     3961 attttttgag tgatcttctc aaaaaatact acctgtccct tgctgatttt taaacgagca
     4021 cgagagcaaa accccccttt gctgaggtgg cagagggcag gtttttttgt ttcttttttc
     4081 tcgtaaaaaa aagaaaggtc ttaaaggttt tatggttttg gtcggcactg ccgacagcct
     4141 cgcagagcac acactttatg aatataaagt atagtgtgtt atactttact tggaagtggt
     4201 tgccggaaag agcgaaaatg cctcacattt gtgccaccta aaaaggagcg atttacatat
     4261 gagttatgca gtttgtagaa tgcaaaaagt gaaatcagct ggactaaaag gcatgcaatt
     4321 tcataatcaa agagagcgaa aaagtagaac gaatgatgat attgaccatg agcgaacacg
     4381 tgaaaattat gatttgaaaa atgataaaaa tattgattac aacgaacgtg tcaaagaaat
     4441 tattgaatca caaaaaacag gtacaagaaa aacgaggaaa gatgctgttc ttgtaaatga
     4501 gttgctagta acatctgacc gagatttttt tgagcaactg gatccaggag aacaaaaacg
     4561 attttttgag gaaagttata aattattttc cgaacgatat ggcaagcaaa atattgctta
     4621 tgcaacagtt cataatgatg agcaaacccc tcacatgcat ttaggtgttg tgcctatgcg
     4681 tgatggaaaa ctgcaaggaa aaaatgtgtt taatcgtcaa gaactgttat ggctacaaga
     4741 taaattcccc gagcatatga aaaaacaggg ttttgagttg aagcgtggtg aacgtggctc
     4801 tgaccgtaaa catattgaga cagctaaatt taaaaaacaa actttggaaa aagagattga
     4861 ttttctagaa aaaaatttag cagttaaaaa agatgaatgg actgcttata gcgataaagt
     4921 taaatcagat ttagaagtac cagcgaaacg acacatgaaa agtgttgaag tgccaacggg
     4981 tgaaaagtcc atgtttggtt tgggaaaaga aataatgaaa acagaaaaga aaccaaccaa
     5041 aaatgttgtt atatcggagc gtgattataa aaacttagtg actgctgcga gagataacga
     5101 taggttaaaa cagcatgtta gaaatctcat gagtactgat atggcgagag aatataaaaa
     5161 attaagtaaa gaacatgggc aagttaaaga aaaatatagt ggtcttgtag agcgatttaa
     5221 tgaaaatgta aatgattata atgagttgct tgaagaaaac aagtctttaa agtctaaaat
     5281 aagcgattta aagcgtgatg tgagtttaat ctatgaaagc actaaggaat tccttaagga
     5341 acgtacagac ggcttaaaag cctttaaaaa cgtttttaag gggtttgtag acaaggtaaa
     5401 ggataaaaca gcacaattcc aagaaaaaca cgatttagaa cctaaaaaga acgaatttga
     5461 actaactcat aaccgagagg taaaaaaaga acgaagtcga gatcagggaa tgagtttata
     5521 aaataaaaaa agcacctgaa aaggtgtctt tttttgatgg ttttgaactt gttctttctt
     5581 atcttgatac atatagaaat aacgtcattt ttattttagt tgctgaaagg tgcgttgaag
     5641 tgttggtatg tatgtgtttt aaagtattga aaacccttaa aattggttgc acagaaaaac
     5701 cccatctgtt aaagttataa gtgaccaaac aaataactaa atagatgggg gtttctttta
     5761 atattatgtg tcctaatagt agcatttatt cagatgaaaa atcaagggtt ttagtggaca
     5821 agacaaaaag tggaaaagtg agaccatgga gagaaaagaa aatcgctaat gttgattact
     5881 ttgaacttct gcatattctt gaatttaaaa aggctgaaag agtaaaagat tgtgctgaaa
     5941 tattagagta taaacaaaat cgtgaaacag gcgaaagaaa gttgtatcga gtgtggtttt
     6001 gtaaatccag gctttgtcca atgtgcaact ggaggagagc aatgaaacat ggcattcagt
     6061 cacaaaaggt tgttgctgaa gttattaaac aaaagccaac agttcgttgg ttgtttctca
     6121 cattaacagt taaaaatgtt tatgatggcg aagaattaaa taagagtttg tcagatatgg
     6181 ctcaaggatt tcgccgaatg atgcaatata aaaaaattaa taaaaatctt gttggtttta
     6241 tgcgtgcaac ggaagtgaca ataaataata aagataattc ttataatcag cacatgcatg
     6301 tattggtatg tgtggaacca acttatttta agaatacaga aaactacgtg aatcaaaaac
     6361 aatggattca attttggaaa aaggcaatga aattagacta tgatccaaat gtaaaagttc
     6421 aaatgattcg accgaaaaat aaatataaat cggatataca atcggcaatt gacgaaactg
     6481 caaaatatcc tgtaaagggt tctgttgcaa agttttaaat aaagaataaa atcc
//
LOCUS       DACEEZ010000243         6505 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20313_2017
            SAMN12639966-rid9879223.guided.117, whole genome shotgun sequence.
ACCESSION   DACEEZ010000243 DACEEZ010000000
VERSION     DACEEZ010000243.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639966
            Sequence Read Archive: SRR10023158
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6505)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6505)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741683.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741683.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:44:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,016
            CDSs (total)                      :: 2,962
            Genes (coding)                    :: 2,889
            CDSs (with protein)               :: 2,889
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 36 of 73
            Pseudo Genes (incomplete)         :: 29 of 73
            Pseudo Genes (internal stop)      :: 18 of 73
            Pseudo Genes (multiple problems)  :: 8 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6505
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639966-rid9879223.guided.117"
                     /isolate="E20313_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            <1..110
                     /locus_tag="IWO89_002972"
     CDS             <1..110
                     /locus_tag="IWO89_002972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="family 1 glycosylhydrolase"
                     /protein_id="HAQ0457642.1"
                     /translation="VYVDQDETQEGSLERYKKDSYYWYQKLIEENGENL"
     gene            299..2746
                     /locus_tag="IWO89_002973"
     CDS             299..2746
                     /locus_tag="IWO89_002973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HAQ0457643.1"
                     /translation="MAQKKRPTKKRKKTKKTKKQKQQQEQMLTIGVGFALILFAVFGF
                     LKLGFLGILIANGFRIIAGNTYQILCLLLAVLGFWIVIKNTEFSIGKNRRWFGGILFY
                     FGILLLLHAHLFGKLHTGEPNIMGTTWDLLASDIKQSQVDNNVGGGMIGAILYHFTYF
                     LIAQPGSYLAAVLLLAGGAFLFSNLEGYQLLNGIQSIGERVQELLEGDPAKQARKQAA
                     KEERMKQRAEAKEARRLAAKEAAEKEAAEYEKKKAVSQQRENTADEWQEPENTEPEQL
                     SFVPIDSFQENIHPANLEKPVPDTPKQTNTAEGFSDELPEDDGTSLEFEIEAEQENQD
                     YELPTVDLLDSIPTVDQSDEYKKIEKNIGVLEQTFQSFGVDAKVVKASLGPSVTKFEV
                     QPAVGVKVSKIVNLTDDIALALAAKDVRMEAPIPGKSLIGIEVPNGKISMVSFREIIE
                     AQPNHPDKLLEVPLGRDVSGRVQTADLSKMPHLLVAGSTGSGKSVAINGIITSILMRA
                     KPHEVKLMMIDPKMVELNMYNGIPHLLTPVVTNPRKAAQALQKVVQEMEERYEKFAAT
                     GVRNISGYNEFVQQKNLENGTKHPTLPFIVVIVDELADLMMVASNEVEDAIIRLAQMA
                     RAAGIHMILATQRPSVDVITGIIKANVPSRMAFAVSSGTDSRTIIDSNGAEKLLGRGD
                     MLFLPMGENKPIRVQGAFISDHEVERVVQFVTDQQEAHYEEKMMPTDEVETTGAPEQP
                     QDELFEEAKALVVEMQTASISLLQRRFRIGYNRAARLVDELEAHGVVGPSEGSKPRKV
                     FIEQEETSETLPDDNDM"
     gene            2976..4043
                     /gene="rlmN"
                     /locus_tag="IWO89_002974"
     CDS             2976..4043
                     /gene="rlmN"
                     /locus_tag="IWO89_002974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2503)-C(2))-methyltransferase
                     RlmN"
                     /protein_id="HAQ0457644.1"
                     /translation="MEKQSIYGLTNEELINWFIENGEKKFRAAQVWEWLYQKRVSNFT
                     EMTNLSKQLIEKLSEHFIINPLKQMVVQEASDGTVKYLFELPDKNMIETVLMRQEYGL
                     SVCVTTQVGCNIGCTFCASGLLKKNRDLTAGEIVAQIMMVQHYFDERNLGERVSHVVV
                     MGIGEPFDNYDNVMDFLHIINDAKGLAIGARHITVSTSGLAHKIKEFANNGLQVNLAI
                     SLHAPNNEVRTSIMRINRSFPIEKLMEAVDEYLEKTNRRITFEYIMLNQVNDRPEHAQ
                     QLADLLKDKKKLAYVNLIPYNPVSEHDQYSRSPKADVLRFYDVLKKNGVNCVIRKEHG
                     TDIDAACGQLRSKQMKKTVAK"
     gene            complement(4236..5339)
                     /locus_tag="IWO89_002975"
     CDS             complement(4236..5339)
                     /locus_tag="IWO89_002975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase family
                     protein"
                     /protein_id="HAQ0457645.1"
                     /translation="MSQSVFLPNYTIGTDAYEKANGIVKEYGTKVVLIGGKTALSKAE
                     NKIKVALTQEIEVVASLWYGGNSTYGNIEQLIQHESVQKADVLFAVGGGRACDTVKVI
                     GEKLAKPVFTFPTIGSNCSPVTAVCVIYNEEGVMDGLFFPSKPPVHTFIDTAIIAEAP
                     DKYLWAGIGDALSKEVESTFSARGDDLGYTDRLGIQIGKMCIDRLLTYGTQALKDSAD
                     DRPSYAIEQVVSDIIVTTGLVSVLVNNNYNSAMAHSFYYGTTVLEKMEGYLHGASVSY
                     GVLTLLMMDQQFDLFDKIYVFMKKNQLPVSLTDMNLNVTTDLEAILDKAMATNDIKHV
                     PYEITREKFKQAILDVETYHATQESQQGYKKIS"
     gene            5892..6440
                     /gene="aac(6')"
                     /locus_tag="IWO89_002976"
     CDS             5892..6440
                     /gene="aac(6')"
                     /locus_tag="IWO89_002976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside N-acetyltransferase AAC(6')-Ii"
                     /protein_id="HAQ0457646.1"
                     /translation="MIISEFDRNNPVLKDQLSDLLRLTWPEEYGDSSAEEVEEMMNPE
                     RIAVAAVDQDELVGFIGAIPQYGITGWELHPLVVESSRRKNQIGTRLVNYLEKEVASR
                     GGITIYLGTDDLDHGTTLSQTDLYEHTFDKVASIQNLREHPYEFYEKLGYKIVGVLPN
                     ANGWDKPDIWMAKTIIPRPNSQ"
BASE COUNT         2186 a         1110 c         1394 g         1815 t
ORIGIN      
        1 ttgtatatgt tgatcaagac gaaacacaag aaggatcttt ggaacgatat aagaaagaca
       61 gttattactg gtaccaaaaa ctgattgaag aaaatggaga gaacctttag ttcacatact
      121 taaaacaaga aggtacggct ggatatggcc gtgccttttc ttgataaaat catttagaaa
      181 ttaagaaaaa ctgtaaaata agctgaaaaa tgaaagaaag cgtcagttct ttcaaacaaa
      241 actttagttc ccttactttt ttttgtataa tgaaacttga tgaaatgagg gataataaat
      301 ggcacaaaag aaacggccta ctaaaaaaag gaaaaagacg aaaaaaacga aaaagcaaaa
      361 acagcagcag gaacaaatgc tgacgattgg tgttgggttt gccttgattt tatttgctgt
      421 cttcggcttt ttaaaattag gttttttagg aatattgatt gcaaacggct ttcggatcat
      481 tgcaggaaat acttaccaaa tcctctgtct tttattagcc gtattaggat tttggattgt
      541 cattaagaat acagagtttt caattggtaa aaaccgacgc tggtttggcg gcatactctt
      601 ttatttcggt atcttattat tgttacatgc ccatttattt ggcaaactcc atactgggga
      661 accaaatatc atggggacga catgggattt gttagccagt gatataaaac aaagtcaagt
      721 agacaataac gttggtggtg ggatgattgg agcaatcctt tatcatttta cttacttctt
      781 gattgcacag cctggaagtt atttagcagc agtgctatta ctggcaggag gagcattcct
      841 gttcagtaat ctggaaggct atcagctgtt aaatggtatc caatcaattg gagaacgagt
      901 acaggaatta ttggaaggtg atcctgccaa gcaagctcga aagcaagcgg caaaggaaga
      961 gagaatgaaa cagcgcgcag aagcgaaaga agcaaggcgc ttagctgcaa aagaagctgc
     1021 agaaaaagaa gctgcagaat atgagaagaa aaaagcggtg agccagcaaa gggaaaatac
     1081 tgctgatgaa tggcaggaac cagaaaatac agagccggag cagctaagtt ttgttccaat
     1141 cgacagtttt caagaaaata tccatcctgc gaatctagag aaaccagtac cagatacacc
     1201 aaaacagacc aatacagcag aaggtttttc agatgagttg ccagaggatg acggtacctc
     1261 attagaattt gagattgaag cagaacagga aaatcaagac tatgaattac cgacagttga
     1321 tttgcttgat tctataccaa ctgtggatca aagtgatgag tataaaaaaa ttgaaaaaaa
     1381 tatcggcgta ttagaacaaa ctttccaaag tttcggtgtc gatgccaagg tcgtcaaagc
     1441 cagcttagga ccatccgtca caaaattcga agtccagccg gctgtagggg taaaagtaag
     1501 taaaattgtg aacttaacag atgatatcgc gcttgcttta gcggcgaaag atgtccggat
     1561 ggaagcaccg attccaggta aatcactgat tggtattgaa gtaccaaatg gaaagatcag
     1621 tatggtttcc tttagagaaa tcatcgaagc acaaccaaac cacccagata aattactaga
     1681 agtaccttta ggacgtgatg tttctggacg ggtacaaacg gctgatcttt cgaagatgcc
     1741 tcacttactc gtggctggtt cgactggtag tgggaagtca gtagcaatta atgggattat
     1801 cacaagtata ttaatgcgtg ctaagcccca tgaagtaaaa ctgatgatga tcgatccgaa
     1861 aatggttgaa ttgaatatgt acaacggcat acctcatctg ttgacgccgg tcgtgacgaa
     1921 ccctagaaaa gcagcgcaag ccttacaaaa agtagtacaa gaaatggaag aacgatatga
     1981 aaaattcgcg gcaaccggag taagaaatat ttctggttac aatgaatttg ttcaacagaa
     2041 aaacttggaa aacggaacga aacatccaac cttaccattc atcgtagtga tcgtagatga
     2101 attagctgat ttgatgatgg tagccagcaa tgaagttgaa gatgcgatta ttcgtttagc
     2161 gcaaatggct cgggcagcgg ggattcatat gatcctagct actcaacgtc caagtgtgga
     2221 tgttatcaca ggaatcatca aagcaaatgt accttccaga atggcatttg cggtatcaag
     2281 cggtacagat tctcggacga tcattgattc taatggggca gaaaagctct tagggcgagg
     2341 agatatgcta ttcttgccaa tgggtgagaa taaaccgatc cgtgtgcaag gagcattcat
     2401 ttctgaccac gaagtagaac gggtcgttca atttgttaca gatcagcaag aagctcatta
     2461 tgaagagaaa atgatgccga cagatgaagt tgaaacgaca ggtgctccag aacagccaca
     2521 agatgagctg tttgaagaag caaaagcact tgtcgttgaa atgcaaacgg caagtatttc
     2581 ccttctccaa agaagatttc ggatcggtta caaccgagca gcacgtttag tagatgaact
     2641 ggaagctcat ggagttgttg gcccgtcaga aggaagcaag cctcggaaag tattcattga
     2701 gcaggaagaa acatcagaaa cgttgccgga cgataatgac atgtaaaact tgtgtgatga
     2761 agggttgaaa aacgaataac caataaaaag acaaggcatg atgataccaa aagaaaaaat
     2821 ggtttttttc catcttttct aatacggtat gatgtcttgt ctttttagtt tttgtgagat
     2881 taaagttcta ttcgatttat ttgtgaagta ttgcattaag gaaatgaatc atgtatagta
     2941 tgtagcgaaa ctttgagaaa aagagggaaa atactttgga aaaacagtcc atttatggct
     3001 tgacgaatga agaattaatc aattggttta tagaaaacgg cgagaaaaaa ttccgagctg
     3061 cccaagtttg ggaatggttg tatcaaaaac gtgtaagtaa tttcacagaa atgacgaatc
     3121 tatcgaaaca attgatcgaa aaattatcgg agcattttat catcaatcct ctaaaacaga
     3181 tggtcgtcca agaagcttcg gatggtacag taaaatattt gtttgaactc cctgataaaa
     3241 acatgattga gactgtactg atgagacaag aatatggctt gtcagtttgt gtgacgacac
     3301 aagtaggatg taatatcggc tgtacgtttt gtgcaagcgg gctgctcaaa aagaaccgtg
     3361 atctgactgc tggagaaatt gttgcacaga tcatgatggt ccaacattat tttgatgagc
     3421 gcaatcttgg cgaacgcgtt tcacatgtcg ttgtaatggg gatcggtgag ccatttgata
     3481 attacgacaa tgtaatggac tttttgcata tcatcaatga tgccaaagga ttggcaattg
     3541 gtgctcggca cattacagtt tccactagtg gattagcaca caaaatcaag gaattcgcta
     3601 ataacggctt gcaagtcaat ctggctattt ccttgcacgc gccgaataat gaggtgcgaa
     3661 catcgatcat gcggatcaac cgaagtttcc caattgagaa attgatggaa gctgtggatg
     3721 agtatttaga gaaaacaaac cgtcgaatca cgtttgaata tatcatgttg aatcaagtaa
     3781 atgatagacc agagcatgcg caacaattag cagatctatt aaaagataag aaaaaattag
     3841 catatgtcaa cttgattcca tataatccag tcagtgaaca tgatcaatat tctcgcagtc
     3901 caaaagctga tgtattgaga ttttatgatg tattgaagaa aaacggcgta aactgtgtga
     3961 tacgtaaaga acatggtaca gatattgatg cagcttgcgg acaattgcga agcaagcaaa
     4021 tgaaaaaaac agttgccaaa taatcgtatc ctactcgttt tttcaattac cataaaagga
     4081 gcagttttct taattaagga actgttcaac ttaaataaga gaaaagattc tatagcgaaa
     4141 gctataaagc tttttttgac aagaaaaacc ctcatcaagc aaagaaatgt cctcaatttg
     4201 atgacagcct gcttgatggg ggttttagca attatttaac tgattttctt atatccttgc
     4261 tggctttctt gtgtagcatg ataagtttct acgtcaagaa tggcctgttt aaatttttct
     4321 cgggtgattt cataaggcac atgtttaata tcatttgttg ccatggcttt atctaagatt
     4381 gcttctaaat cggtcgtgac attgagattc atgtcggtta acgatactgg aagttggttc
     4441 tttttcataa acacatagat tttatcaaac aaatcgaact gctgatccat catcaataag
     4501 gtcagtacac cataactgac agatgctccg tgaaggtatc cctccatttt ttctagaaca
     4561 gtcgtaccgt aatagaatga gtgtgccata gcagaattat agttattgtt aactaaaaca
     4621 gaaaccaatc cggtagtaac aatgatatcc gaaacgactt gttcaattgc atatgaggga
     4681 cgatcgtcgg cactatcttt caatgcttgc gtaccataag tcaagagacg atcaatgcac
     4741 atttttccga tttggattcc taatctatcc gtgtagccta gatcatcgcc acgagcagaa
     4801 aaagtagatt cgacttcttt ggataaagca tcgccaatac ctgcccataa gtatttatca
     4861 ggtgcttcgg caataattgc tgtatcaatg aaggtgtgta caggtggttt tgaaggaaag
     4921 aagagtccgt ccattacgcc ttcttcgtta tagatgacac ataccgcagt gacgggcgaa
     4981 cagttcgaac caatagtggg aaaggtgaag actggtttag ctagtttttc accaatgact
     5041 tttacagtat cgcaagcacg gccaccaccg actgcaaaca ggacgtctgc tttttggaca
     5101 ctttcatgct ggatcaactg ttcaatattt ccataagttg aattaccccc gtaccagaga
     5161 ctggcgacaa cctctatttc ctgtgtcaaa gctactttta ttttgttttc tgcctttgaa
     5221 agtgcagtct tccctccaat cagcactact tttgtcccat attctttgac gattccattt
     5281 gctttttcat acgcatctgt tccaatggta taatttggta agaaaacact ttgactcata
     5341 ttcatttcct tctttcttcg ttgaaacggg ttaaaatttt aaagagtgat cgatctgact
     5401 gcggctgtag atggagtaga ggtgaaccta agacaggata agtgatgaat aaggtggcat
     5461 gaacaccttg aaatcatcaa taagtatcct aaaaacgaac aagtacaaaa acattgaaaa
     5521 cgacaataat gaatttttgt tcctcttagt atttctaccg attccctttg cttttttgta
     5581 attatctaaa aattctaaaa attaagatta ttatatgaaa acaggtttta tttgtcaatt
     5641 cataattggt tttttttaaa gcaaaagata atctatatca tgatgaacaa acagagtttt
     5701 ttcctccaat tgatggcttt cctgatcttt cctattttga ataaagtgtt tctgtataat
     5761 ttttttattt tataagtaag ataaataatg aaagaataga gatactgatt ggtagctaaa
     5821 gatttttgag cgagaacatt tcgttttttc aaaaggactg ttagaataag caagaacaag
     5881 ggggaagacg tatgataatc agtgaatttg accgtaataa tccagtattg aaagatcagc
     5941 tttctgattt actgagactg acttggccgg aagaatatgg agacagctcg gcagaagaag
     6001 tagaagaaat gatgaatcca gaacgaatcg cggtagcagc ggtagaccaa gatgagttag
     6061 taggatttat tggtgcaatc cctcaatacg gtatcacagg ttgggaattg catccattag
     6121 ttgtagaaag ctcccgacga aagaaccaaa taggtactcg attagtcaat tacttagaaa
     6181 aagaagtagc ttccagagga ggaatcacga tttatttagg tacggatgat ttagaccatg
     6241 gaacaacgtt aagtcaaacg gacctgtatg agcatacatt tgataaagtg gcttccatcc
     6301 agaaccttcg tgaacatccg tatgaattct atgaaaaatt aggttataaa atcgtaggtg
     6361 tcttaccaaa tgcaaatggc tgggacaaac cggatatttg gatggcaaaa acgattattc
     6421 ctcgaccaaa ttctcaataa taaaaagagg ttgtgacaga agtaatcagc ttcaagaaat
     6481 aagaaggaat ttacgaaaat tactt
//
LOCUS       DACEEZ010000244         6291 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20313_2017
            SAMN12639966-rid9879223.guided.122, whole genome shotgun sequence.
ACCESSION   DACEEZ010000244 DACEEZ010000000
VERSION     DACEEZ010000244.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639966
            Sequence Read Archive: SRR10023158
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6291)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6291)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741683.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741683.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:44:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,016
            CDSs (total)                      :: 2,962
            Genes (coding)                    :: 2,889
            CDSs (with protein)               :: 2,889
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 36 of 73
            Pseudo Genes (incomplete)         :: 29 of 73
            Pseudo Genes (internal stop)      :: 18 of 73
            Pseudo Genes (multiple problems)  :: 8 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6291
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639966-rid9879223.guided.122"
                     /isolate="E20313_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            102..1223
                     /locus_tag="IWO89_002977"
     CDS             102..1223
                     /locus_tag="IWO89_002977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HAQ0457647.1"
                     /translation="MDILAAIDSFKGSASSFELNQAVLNGWEDRKGRKINVPIADGGE
                     NTAEAIYYALRGTWRTIDGMDLLFRQRKISYLLTTWQGKNTAVIESAQALGLDLLDKP
                     TDQTIRLASSYALGELIKDALLQEVEQIIVTLGGSGCSDGGLGLLQSLGASLEGYTEG
                     NPLLSTKKLHFDRRNVLVSKVKLIIAADVTNPYTGQQGAAWQFGRQKGGIDTTLAFLD
                     KQAGKIAQQIKDFWEVDLDDIPGSGAAGGIGGALFLLGAKMVSGFDLVSQITGLEKKI
                     EQADVIITGEGRIDQQTIHGKVPYGVAVLAKKYRKPIIAICGSRDQELEGLSDFLPIV
                     LSIHLGPIGLEEAMEHRQTLEKTKILGQTLSRFFTILPK"
     gene            1481..2983
                     /locus_tag="IWO89_002978"
     CDS             1481..2983
                     /locus_tag="IWO89_002978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F type ribosomal protection protein Eat(A)"
                     /protein_id="HAQ0457648.1"
                     /translation="MSKIEIKNLTFGYDSQGTLLFEQANLNFDTQWKLGLIGRNGRGK
                     TTLLNILQNKLPYQGQVIHQQEFAYFPQQTKDKERLTYYVLNDITDFEIWEIERELQL
                     MQTDPEILWREFSTLSGGEKTKVLLALLFVDDTHFPLIDEPTNHLDISGRKQVAAYLK
                     KKKQGFIVVSHDRGFIDEVVDHVLAIEKSQLELYQGNFSIYEEQKKLRDEFEMAQNEK
                     LKKEVSRLKKTAAEKAEWSRSREGDKTKKQVGFIDTESRRVNKGAVGADAARTMKRSK
                     AIVNRMETQISEKEKLLKDIEYIDSLTMNSQASHHKRLLSVEDLQLGYENLLFEPIHF
                     TIEPHQRVAISGPNGAGKSSIIHYLLGAFNGKVIGEKYQPKHLSISYASQNYEDNRGT
                     LAEFAEKNQVDYQAFLNNLRKLGMERDVFHNKIEQMSMGQRKKVELAKSLSQPAELYT
                     WDEPLNYLDVFNQEQLEQLILNVKPAMLLVEHDQTFLDKVSTEIISLERI"
     gene            3050..3577
                     /locus_tag="IWO89_002979"
     CDS             3050..3577
                     /locus_tag="IWO89_002979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HAQ0457649.1"
                     /translation="MLYVTRHGETTWNAQGLVCGRADVPLTEKGQMQAQKLAEKVVDL
                     PVPITKIIHSPLQRARDTAQAVADRLSLPLTVDERLVEMDFGDYDGLPSKDENFQKAR
                     LAFAVRFPNGESVLDVYARIVPLLKECIEDEENVYLLVCHNALIRVINAYFHPMPNEG
                     FFTFMVDNTELVSYE"
     gene            3788..4849
                     /locus_tag="IWO89_002980"
     CDS             3788..4849
                     /locus_tag="IWO89_002980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF871 domain-containing protein"
                     /protein_id="HAQ0457650.1"
                     /translation="MFGFSIFMNDSLSEEKKQYIRQMASNGFVGIFTSMHIPEDDVSA
                     YQKRLIELGSEAKKNKLELMVDISGEALERAGFSLEDLTPLKAIGVTGLRMDYHISNQ
                     QIADWSHQLKISLNASTITPKDIDELKEAEADFSQIEAWHNYYPRPETGLDKEWYQKK
                     NQWLKIQGLLVQGFVPGDTELRGPLYQGLPTLEEHRGIHPLAGALDLLASNTDIVYIG
                     DAGLSENVQEQFASFQKEQTVLLHTEPVDEEFYEYILGKHTNRQDDARDVIRSVDARF
                     REIPPIPARNTATRMKGSITLDNEKYLRYMGEIQLTKYDLPADEKVNVVAKVIKEELP
                     LINQIKAGMNYQFIRKEGR"
     gene            4852..5748
                     /gene="murQ"
                     /locus_tag="IWO89_002981"
     CDS             4852..5748
                     /gene="murQ"
                     /locus_tag="IWO89_002981"
                     /EC_number="4.2.1.126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramic acid 6-phosphate etherase"
                     /protein_id="HAQ0457651.1"
                     /translation="MSKINLENLTTERRNQETFGLDEMSISQAVQLMNKEDQKVAAAV
                     EKELTSIEPVIANTIESFKKGGRLIYMGAGTSGRLGVLDAAECVPTFGVEPEMVIGLI
                     AGGEEAMTVAVEGAEDSYELGQEDLIKLNLTKNDIVIGIAASGRTPYVIGGLDYAKSI
                     GAVTGTISCNKQAEISKHADFPIEVDAGPEFLTGSTRLKSGTAQKLILNMISTISMIG
                     IGKVFNNLMVDVKPTNEKLIERSKRIIMQATEADYETAEKYFEEADQNVKLAIVMILT
                     NSDKEEAQAKLAEANGFVKGTL"
     gene            5762..>6291
                     /locus_tag="IWO89_002982"
     CDS             5762..>6291
                     /locus_tag="IWO89_002982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIB"
                     /protein_id="HAQ0457652.1"
                     /translation="MAKNELTLEELAKQIYKGAGGMGNVESIVHCMTRVRMKIINDDL
                     VNVSELKAIPGVLGVVEDEQLQVIIGPGKVNKVAQQMVDLAGVKLGEKLSSVSTATAN
                     SSASGKDRVNERAQEMKAAQKSKQKPSKIKSVLKDISNIFVPMIPAFVGTGIVAGIAA
                     VLANLVTAGTLDAATW"
BASE COUNT         2178 a         1071 c         1409 g         1633 t
ORIGIN      
        1 tttttatttt aaatggctaa gttcattata aaatccgcca ttgttttgtt tgaaaaaaat
       61 gaaaaaaagt gtcttactta agaagaaagt gagggataca gatggatatt ttggcggcaa
      121 ttgattcatt taaaggaagt gcttcttcat ttgaactaaa tcaggcagtt ctaaatggct
      181 gggaagatcg taaaggaaga aaaatcaatg tgccgattgc agatggcgga gaaaatactg
      241 cagaagcaat ctattacgcg ctcaggggaa catggcgtac cattgatggg atggatctct
      301 tattccgtca gcgaaaaatc agctatttat tgacaacttg gcaaggaaaa aacaccgcag
      361 tcatcgaatc tgcacaagca ttggggttag atctacttga taaaccaacg gatcaaacta
      421 tccgacttgc ttccagttat gccttaggag aacttattaa agatgcactg cttcaagaag
      481 tagaacaaat tattgtcact ttaggaggaa gcgggtgtag cgatggtggt ctaggattac
      541 tccaatcctt gggtgcttct ttagaagggt acacagaagg aaatccttta ttatctacta
      601 aaaaacttca ttttgatcga agaaacgttc ttgtttctaa agtaaaattg attatcgctg
      661 cagatgttac caatccatat acaggtcaac aaggagctgc ttggcaattt ggaagacaaa
      721 aaggaggaat agatacgacg ctagctttct tagataaaca agcaggaaag attgctcagc
      781 aaataaaaga tttctgggag gtggatttag atgatattcc aggaagtgga gcagcaggcg
      841 gtattggtgg agcccttttt ttgttgggag cgaagatggt ttctggtttc gatttagtca
      901 gccaaatcac tggattggaa aaaaagatcg aacaagcaga tgtcatcatt acaggagaag
      961 ggcggatcga tcagcaaacg atccacggaa aagttccata cggtgtagca gtgcttgcaa
     1021 aaaaatatcg aaaaccaata atcgctattt gcggaagtag agaccaagag ctagaaggac
     1081 tttctgattt tctcccaatt gtcttaagta ttcatttggg tccaataggt ttagaagaag
     1141 caatggaaca caggcaaaca ttagaaaaaa caaaaatctt aggacagaca cttagcagat
     1201 ttttcaccat cctcccgaag tagttgacaa aatcattttc tttatgctat cgtaatttca
     1261 aatataggac aaagtgaggg atccgtgcat tgaaaaatta agtcgattga tgaaagctgc
     1321 attcagactg taccttgtgg agacaaggaa gcagtgtgca gattttcggg attggcttgg
     1381 ttttttcgat gtatgggtct ttttgtgtgt atccaaccct tgcatcctct ttagccagtt
     1441 ctgaaaatag gactggtctt tttttacgag gtgatggaaa atgtctaaaa tcgaaataaa
     1501 aaatctgaca ttcggctacg acagccaagg cacattatta tttgaacaag caaatctaaa
     1561 ttttgacaca caatggaaac taggacttat cggacgaaac ggtcgaggaa agacaacttt
     1621 actgaatatt ctacaaaaca aactacctta tcaagggcaa gtaatccatc agcaagaatt
     1681 tgcctatttc ccgcaacaga caaaagataa agaacgttta acctattacg tgttaaatga
     1741 tattacggat tttgagatat gggaaatcga aagagagctc caattgatgc aaacagatcc
     1801 tgaaatctta tggagagaat tcagcacact atcgggggga gagaagacaa aagtcctact
     1861 ggcactttta tttgtggatg acactcattt cccgttaatc gatgaaccaa cgaatcattt
     1921 ggatatctct ggtagaaaac aagtagcggc ttatttgaaa aagaaaaaac aaggcttcat
     1981 cgtggtcagc catgaccggg gatttatcga tgaagtagtg gaccatgttt tagcaatcga
     2041 aaaaagtcaa ctggaacttt atcaagggaa tttctctatc tatgaagaac agaaaaaact
     2101 tcgtgatgaa tttgaaatgg ctcaaaatga aaaattgaaa aaagaagtca gtaggctaaa
     2161 gaaaacagca gctgaaaaag ccgaatggtc tcgttcccga gaaggagata aaacaaagaa
     2221 acaagtcgga ttcatcgata ctgaatctag acgagtgaat aaaggagcag tgggtgctga
     2281 tgctgcacgg acgatgaaac gatccaaagc aatcgtgaat cggatggaga cccagatcag
     2341 cgagaaagaa aaactattaa aagatatcga atatatcgat tctttgacga tgaatagcca
     2401 agcgtctcac cataagcgac ttttaagcgt agaagatctt caattagggt atgaaaatct
     2461 gttattcgag ccaattcatt ttacaatcga gcctcatcag cgggtggcga tttcaggtcc
     2521 taacggtgca ggaaagtcat ccattatcca ttatcttctg ggggcattca acggcaaggt
     2581 tataggagaa aaataccagc caaaacatct gagcattagt tatgcaagcc aaaattatga
     2641 agacaatcga ggaacgttgg cggaatttgc agagaaaaac caagtagact accaagcatt
     2701 tttgaacaac ctccgaaagc ttgggatgga aagagatgtt tttcataaca agatcgagca
     2761 gatgagtatg ggccaacgga aaaaagtgga attggctaaa tctttatcac agccagctga
     2821 actatataca tgggatgaac cattgaatta tttggatgtc ttcaatcaag aacaattaga
     2881 acaactgatc ttgaacgtga aacctgccat gttactagtg gaacatgatc aaaccttcct
     2941 ggataaagta tctactgaga ttatttctct tgagagaatc taacccttgt aatctcaaga
     3001 acaacaaggt atggttaagg ggaactttgc caacgaagga gagaatcaca tgctttatgt
     3061 aacgagacac ggagaaacaa cttggaacgc acaaggatta gtttgcggac gggcagatgt
     3121 gccgctgact gaaaaaggac agatgcaggc acagaaacta gcagaaaagg tagtagattt
     3181 gcctgttcca atcacgaaaa tcatccattc ccctcttcaa cgagcaagag atacagcaca
     3241 agcagttgct gatcgtctca gcttgccgct gaccgttgat gaacgattgg tagaaatgga
     3301 ttttggggat tatgatggac tgccaagcaa agatgagaac tttcaaaaag cacgtttagc
     3361 gttcgctgta cgattcccaa atggggagtc agtcttagat gtgtatgcac ggatcgttcc
     3421 tttattgaaa gaatgcatcg aagacgaaga aaatgtctat ctgttagttt gccataatgc
     3481 cttgataaga gtgatcaatg cttattttca tccgatgcct aatgaggggt tctttacatt
     3541 catggtagat aatacagaat tggtttctta tgagtaatta tgtttttgtg ttatttaaat
     3601 aaaatatttc aaggcactgc ttttgcttag cagtgccttt tttaggttat gacaattctt
     3661 ttgataatat aaactcaacc tttagaaatg aaatttaatt attttaataa aagtattgaa
     3721 attttgtttc tttagatata tactctagtc atagaaatga agacggatac agaaaaagag
     3781 ggataatatg tttggctttt caatatttat gaacgattct ttgtcagaag agaagaaaca
     3841 gtatatccgc caaatggcat ccaatggctt tgtcggtatt ttcacttcca tgcatatacc
     3901 agaagatgat gtttcggcat atcaaaagcg attgatcgaa ctaggaagcg aagcgaaaaa
     3961 gaataaacta gaattgatgg tcgatatttc aggagaggca ttggaacgag cgggtttttc
     4021 attagaagat ctaacgcctt tgaaagcaat tggtgtaaca ggtttaagaa tggattatca
     4081 tatttccaat cagcagattg ccgactggtc acatcaattg aaaatcagtt tgaatgctag
     4141 cacgatcacg cctaaagata tcgatgaatt gaaagaagca gaagctgatt tctcacagat
     4201 tgaagcttgg cataattatt atccacgtcc cgaaacaggg ctggacaaag aatggtatca
     4261 gaaaaaaaat caatggctca aaattcaagg tctccttgtc caaggttttg taccaggaga
     4321 tacagagttg agaggcccac tatatcaagg attgccaact ttggaagaac atcgagggat
     4381 ccatcctctt gcaggcgctc tagatctatt agcaagtaac acggatattg tttacatcgg
     4441 agatgcaggt ctgtcagaaa acgttcagga gcaatttgct tctttccaaa aagaacagac
     4501 agtcttgctc catacggaac ctgtagatga agaattttac gaatatatat tagggaagca
     4561 tacgaatcgg caagacgatg caagagatgt catacgaagc gtagatgcac gttttcgaga
     4621 gattccgcct atcccagcaa gaaatacagc tacgcgtatg aaagggtcta ttactttaga
     4681 taacgaaaag tatttgcgct atatgggtga gatccaatta accaaatatg atttgccagc
     4741 ggatgaaaaa gtgaatgtag tagctaaagt tatcaaagaa gagctaccat tgatcaacca
     4801 aatcaaagct ggtatgaact atcaatttat aagaaaagaa ggaagataaa aatgagcaaa
     4861 atcaatttag agaacctaac aacggaacgc agaaaccaag aaacttttgg attagatgag
     4921 atgagtattt ctcaagccgt tcaactaatg aacaaagaag atcagaaagt ggccgctgcg
     4981 gttgaaaaag aactgacaag tatcgaacca gtgattgcta acacgattga gtcctttaaa
     5041 aaaggtggac gtctgattta tatgggagcc ggaacaagtg gaagactagg tgtgttagat
     5101 gctgctgaat gcgtaccgac attcggtgta gaaccggaaa tggtcatagg tctaattgct
     5161 ggtggagaag aagcaatgac tgtagctgtc gaaggggccg aagattctta tgagctaggg
     5221 caagaagatc taatcaagct gaatttgaca aaaaatgata tagttattgg tatcgcagct
     5281 agcggtcgta caccatatgt gatcggtgga ttagattacg caaaatcgat cggtgctgtc
     5341 acaggaacga tttcttgcaa taaacaagcc gaaatcagta aacacgcgga tttcccaatc
     5401 gaagtcgatg caggtccaga atttttgaca ggctctactc gattgaagtc aggaacagca
     5461 caaaaactta tcttgaatat gatttctact atcagtatga ttggtatagg taaagtattc
     5521 aataacttga tggtcgatgt caaaccaaca aatgaaaaac taatcgaacg ctcaaaacga
     5581 atcatcatgc aagcgacaga agctgattac gaaacagctg aaaagtactt tgaagaagca
     5641 gatcaaaatg ttaagctggc tatcgtcatg attttgacaa atagtgataa agaggaagct
     5701 caagcaaaat tagctgaagc aaacggattt gtaaaaggga ccttatgagg gggaaaagac
     5761 catggcaaaa aacgaactga ctttggaaga actggcaaaa caaatttata aaggggcagg
     5821 cggcatgggc aatgtagaaa gcattgtcca ctgcatgact cgtgtgcgta tgaagatcat
     5881 caatgatgat cttgtaaatg tctctgaatt aaaagcgatt ccaggagtgc ttggtgtagt
     5941 agaagatgaa cagcttcaag tcatcatcgg acctgggaaa gtgaacaaag tagcacagca
     6001 aatggttgat ttggcaggcg tgaaattagg agaaaaactt tcttctgtaa gtacagctac
     6061 agcaaattca tcagcatctg gaaaagaccg tgtaaacgaa cgggcacaag aaatgaaagc
     6121 ggcacaaaaa tcgaaacaaa aaccatctaa aatcaaatcg gtattaaaag acatttctaa
     6181 tatctttgtg ccaatgattc ctgcatttgt cggtactggg atcgtggcag ggattgccgc
     6241 agttttagca aacttagtca cagccggaac acttgatgca gcaacatggc a
//
LOCUS       DACEEZ010000245         5426 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20313_2017
            SAMN12639966-rid9879223.guided.135, whole genome shotgun sequence.
ACCESSION   DACEEZ010000245 DACEEZ010000000
VERSION     DACEEZ010000245.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639966
            Sequence Read Archive: SRR10023158
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5426)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5426)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741683.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741683.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:44:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,016
            CDSs (total)                      :: 2,962
            Genes (coding)                    :: 2,889
            CDSs (with protein)               :: 2,889
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 36 of 73
            Pseudo Genes (incomplete)         :: 29 of 73
            Pseudo Genes (internal stop)      :: 18 of 73
            Pseudo Genes (multiple problems)  :: 8 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5426
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639966-rid9879223.guided.135"
                     /isolate="E20313_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            <1..86
                     /locus_tag="IWO89_002983"
     CDS             <1..86
                     /locus_tag="IWO89_002983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000361059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HAQ0457653.1"
                     /translation="RVMELEKENKKLQNQIQKLYGDLYNKE"
     gene            240..1022
                     /locus_tag="IWO89_002984"
     CDS             240..1022
                     /locus_tag="IWO89_002984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000067268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside nucleotidyltransferase ANT(9)-Ia"
                     /protein_id="HAQ0457654.1"
                     /translation="MSNLINGKIPNQAIQTLKIVKDLFGSSIVGVYLFGSAVNGGLRI
                     NSDVDVLVVVNHSLPQLTRKKLTERLMTISGKIGNTDSVRPLEVTVINRSEVVPWQYP
                     PKREFIYGEWLRGEFENGQIQEPSYDPDLAIVLAQARKNSISLFGPDSSSILVSVPLT
                     DIRRAIKDSLPELIEGIKGDERNVILTLARMWQTVTTGEITSKDVAAEWAIPLLPKEH
                     VTLLDIARKGYRGECDDKWEGLYSKVKALVKYMKNSIETSLN"
     gene            complement(1148..1879)
                     /gene="erm(A)"
                     /locus_tag="IWO89_002985"
     CDS             complement(1148..1879)
                     /gene="erm(A)"
                     /locus_tag="IWO89_002985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001072197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2058)-N(6))-methyltransferase
                     Erm(A)"
                     /protein_id="HAQ0457655.1"
                     /translation="MNQKNPKDTQNFITSKKHVKEILNHTNISKQDNVIEIGSGKGHF
                     TKELVKMSRSVTAIEIDGGLCQVTKEAVNPSENIKVIQTDILKFSFPKHINYKIYGNI
                     PYNISTDIVKRITFESQAKYSYLIVEKGFAKRLQNLQRALGLLLMVEMDIKMLKKVPP
                     LYFHPKPSVDSVLIVLERHQPLISKKDYKKYRSFVYKWVNREYRVLFTKNQFRQALKH
                     ANVTNINKLSKEQFLSIFNSYKLFH"
     gene            complement(1937..2024)
                     /locus_tag="IWO89_002986"
                     /pseudo
     CDS             complement(1937..2024)
                     /locus_tag="IWO89_002986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017771940.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ErmCL family antibiotic resistance leader
                     peptide"
     gene            2388..3050
                     /locus_tag="IWO89_002987"
     CDS             2388..3050
                     /locus_tag="IWO89_002987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HAQ0457656.1"
                     /translation="MNTIESQFDKVAEDYDFVNELLNDYSFFVSNMSPKKGRALDIGC
                     GSGLLVEKLASYYDEVVGIDISNQMLDLAKSKRQLTNTVYLNMNAEQLNFNEKFDFIV
                     SRTTFHHLDDIASVIQQMKELLNEEGRIVILDNVSEVETPPTYVYKLGAIQEFLPHCF
                     KFGIKNAIRIYNHNTSKSWLEHLASDKYLSEQNYYDLYEKLLPGCQFHKMGWAMGVVW
                     TK"
     gene            3285..3428
                     /locus_tag="IWO89_002988"
                     /pseudo
     CDS             3285..3428
                     /locus_tag="IWO89_002988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326507.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein"
     gene            3578..3778
                     /locus_tag="IWO89_002989"
     CDS             3578..3778
                     /locus_tag="IWO89_002989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HAQ0457657.1"
                     /translation="MENGTVKWFNAEKGFGFISREDGSDVFVHFSAIQGDGFKTLEEG
                     QAVTFDVEESDRGPQAANVVKA"
     gene            complement(4032..>5426)
                     /locus_tag="IWO89_002990"
     CDS             complement(4032..>5426)
                     /locus_tag="IWO89_002990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease J"
                     /protein_id="HAQ0457658.1"
                     /translation="YAGPLALALITNKLDEHGLLRDAELHEINEDTIIRFRKTSVSFF
                     RTTHSIPDALGIVVKTPSGNIVATGDFKFDFTPVGEPANLHKMARIGEEGVLCLLSDS
                     TNAEIPTFTKSEKTIGHSILKIFSKIEGRIIFASFASNIFRLQQAADAAVATGRKIAV
                     FGRSMENAIVNGQRLGYIKVPDGTFVDAHEINRLPANETMILCTGSQGEAMAALSRIA
                     NGTHRQIQIQPDDTVIFSSSPIPGNTTSVNHLINLLSEAGAEVIHGKINNIHTSGHGG
                     QEEQKLMLRLMKPKYFIPVHGEFRMLKVHASLAQDVGVPAENCFIMENGDVLALTSDS
                     ARPAGHFVAGDVYIDGSGIGDIGNVVLRDRHLLSEEGLVLAVATVDIKKKEILAGPDI
                     LSRGFVYMRESGEMINEAQRILFHALRECLNGPDCSEYKLKEAMTGALQPFLFERTER
                     HPMILPMVMTPDEE"
BASE COUNT         1760 a          893 c         1040 g         1733 t
ORIGIN      
        1 gaagagtaat ggaattagaa aaagaaaata aaaaattaca gaaccaaatt caaaaattat
       61 atggagatct gtataataaa gaataattat taatctgtag acaaattgtg aaaggatgta
      121 cttaaacgct aacggtcagc tttattgaac agtcatttag gtatatgtcc aatctagggt
      181 aagtaaattg agtatcaata taaactttat atgaacataa tcagttacga ggtgaaatca
      241 tgagcaattt gattaacgga aaaataccaa atcaagcgat tcaaacatta aaaatcgtaa
      301 aagatttatt tggaagttca atagttggag tatatctatt tggttcagca gtaaatggtg
      361 gtttacgcat taacagcgat gtagatgttc tagtcgtcgt gaatcatagt ttacctcaat
      421 taactcgaaa aaaactaaca gaaagactaa tgactatatc aggaaagatt ggaaatacgg
      481 attctgttag accacttgaa gttacggtta taaataggag tgaagttgtc ccttggcaat
      541 atcctccaaa aagagaattt atatacggtg agtggctcag gggtgaattt gagaatggac
      601 aaattcagga accaagctat gatcctgatt tggctattgt tttagcacaa gcaagaaaga
      661 atagtatttc tctatttggt cctgattctt caagtatact tgtctccgta cctttgacag
      721 atattcgaag agcaattaag gattctttgc cagaactaat tgaggggata aaaggtgatg
      781 agcgtaatgt aattttaacc ctagctcgaa tgtggcaaac agtgactact ggtgaaatta
      841 cctcgaaaga tgtcgctgca gaatgggcta tacctctttt acctaaagag catgtaactt
      901 tactggatat agctagaaaa ggctatcggg gagagtgtga tgataagtgg gaaggactat
      961 attcaaaggt gaaagcactc gttaagtata tgaaaaattc tatagaaact tctctcaatt
     1021 aggctaattt tattgcaata acaggtgctt acttttaaaa ctactgattt attgataaat
     1081 attgaacaat ttttgggaag aataaagcgt cctcttgtga aattagagaa cgctttatta
     1141 ctttaattta gtgaaacaat ttgtaactat tgaaaataga aagaaattgt tccttcgata
     1201 gtttattaat attagtgaca tttgcatgct tcaaagcctg tcggaattgg tttttagtga
     1261 aaagaacacg atattcacgg tttacccact tataaacaaa agatcgatac tttttgtagt
     1321 ccttctttga aatcaatggt tgatgtcgtt caagaacaat caatacagag tctacacttg
     1381 gcttaggatg aaaatatagt ggtggtactt ttttgagcat ttttatatcc atctccacca
     1441 ttaatagtaa acccaaagct cgttgcagat tttgcaatct tttcgcaaat cccttctcaa
     1501 cgataagata gctatattta gcctgacttt caaaggtaat tcttttgaca atatccgtac
     1561 tgatgttata aggaatatta ccatatatct tatagtttat atgttttggg aaggaaaatt
     1621 ttagaatatc cgtttgaatc acttttatat tctcagaggg gtttaccgct tctttagtca
     1681 cttgacataa gcctccatca atttctatag cagtaactga tcgactcatt ttgactagct
     1741 ctttggtaaa atgtcctttt cctgatccga tttctattac gttgtcttgt ttactgatat
     1801 tcgtgtgatt caatatttct tttacatgct ttttagaagt aataaaattt tgcgtgtctt
     1861 tagggttttt ctggttcatt ataaccttct ccttactggt tataatgaac tgacattaag
     1921 cgttcattat aaccaattaa ttttgatttg gttgataacg aactttatta ataacaaata
     1981 tagaaaaagt acccatactt ttatctcctt tccatttttt tcattatgaa tgagataaag
     2041 taatttctac tactgcgata ctggtgcaca taattgaaac ctccttatgt aactgaatat
     2101 gattgtatca tcttttcatt ttagaataaa cctttatatg taattttgca cttgacactt
     2161 cagatggacg ttttcttcaa aataatatgt tttgaaactt gataagagaa gccactgtct
     2221 ttactaaaaa gtaaaggctg cgataaagga cagtgacttt aataaagttg cactgttgta
     2281 ttgtctttgg ataaaattgt ctaaagggaa gttttatccc caacgaaaaa acatctaaag
     2341 tggtaagtaa tgaaagaatg tcgataaagt aggaatgggg gatctttttg aatacaatag
     2401 aatcacagtt tgataaggtt gcagaagatt acgatttcgt gaatgagctt ttgaatgatt
     2461 attcattctt tgtgtctaat atgtctccaa agaaaggcag agcattagat atcggatgtg
     2521 gctcgggttt gttagtggag aaattagcaa gttattatga tgaagtggta gggattgata
     2581 tttctaatca aatgctcgat cttgccaaat ctaaacgtca actaacaaat acggtctatc
     2641 tgaatatgaa tgcagaacaa cttaatttta atgagaagtt tgattttatt gtaagccgaa
     2701 caacctttca tcatttggat gatatagcca gcgtaataca gcaaatgaag gaactgttga
     2761 atgaggaagg aagaatagtt attcttgata atgtatctga agtcgagaca ccgcctacct
     2821 atgtttataa attaggggca atccaagagt ttttaccgca ttgtttcaaa tttggaataa
     2881 aaaatgcgat tagaatttat aatcacaaca catctaaatc atggcttgag catttagctt
     2941 cagataagta cctatctgaa caaaattatt acgatttata tgaaaagttg ttgcctggat
     3001 gccaattcca taaaatgggt tgggcaatgg gggttgtctg gacaaaataa cttaaagctg
     3061 tggctttgaa aagttgaatt tgtgattgtt ttcagagcta tcgggtgctt tagtataaat
     3121 aacaatgaat aaaaaatcag cctgtatctt atgttactat taagtaattt aagatacagg
     3181 ttttactatt tttgaaataa gtaccttcca cttcaaatac atgtattcaa agtgaaagta
     3241 ttgatttaaa tgattctttt gtaatttact cttgataata tgaaattcca tcgcagcagg
     3301 atatgaattt taccaaaagg atacaaaagt gtggagaaat gatggggatc actgtactgg
     3361 accacttgat catcggacgc aaacgatatt ttagtttgag agaagaagga atgatggaag
     3421 agaagtaagt tgctttcttg caaaaaaaat gaagcaagtg tacaattata ttgtaatttt
     3481 gtttgggaat agaagttgaa acatctgttg cacctctatc gtacacgagc aatgttacaa
     3541 tttaaattag tgcttttggc acgaggagga tattattatg gaaaacggaa cagtaaaatg
     3601 gttcaacgct gaaaaaggat ttggatttat ctcacgtgaa gatggaagcg acgtattcgt
     3661 tcacttctca gctatccaag gtgacggatt caaaacatta gaagaaggtc aagcagtgac
     3721 tttcgacgtt gaagaatcag accgcggccc acaagctgca aacgttgtaa aagcttaatt
     3781 ttatctgtaa atcaaattga tggtagacat caatttttac gagagaacaa agcgagtaac
     3841 aaaaatacct gccacaagag acaatttctc ttgtggcagg tatttttttg tatgaaaaag
     3901 taaataaatt taagaggcta tgaaagagcc accttgtatc aaggaataag aacaaacaaa
     3961 aatggcttct catgtttttc atttgttttc tcttatattc aggacaaggt gctgatttca
     4021 taacctctta tttattcttc atctggtgtc attaccattg gcaaaatcat tggatgtctt
     4081 tctgtacgtt cgaataagaa aggctgaagc gcgccagtca tcgcttcttt caatttatat
     4141 tctgaacagt caggtccatt caaacattct cttaaagcgt ggaataagat tcgctgagct
     4201 tcattgatca tttctcctga ttctcgcatg tagacaaatc cacgagagag aatatctgga
     4261 ccagccaaga tttctttttt cttgatatcc actgtcgcga cagctaatac aagaccttct
     4321 tctgaaagca ggtgacggtc acgtaaaacg acattaccaa tatcaccgat cccgctacca
     4381 tcgatataca catctccagc cacaaaatgc ccagcaggac gcgcagaatc gctagttaac
     4441 gctaaaacat cgccattttc catgatgaag cagttttctg caggaacacc tacatcttgt
     4501 gctagagaag catgtacttt caacatgcgg aattctccgt ggacaggaat aaaatatttt
     4561 ggcttcatca aacgaagcat taatttttgt tcttcttgtc caccgtgacc ggaagtatgg
     4621 atgttattga tttttccgtg aatcacttct gcaccagctt cagacagcaa attgatcaaa
     4681 tgattgacac ttgttgtatt accaggaata ggtgaacttg aaaagatcac tgtatcgtct
     4741 ggctggattt ggatttgacg gtgagtacca ttcgcgatgc ggctcaacgc ggccatcgct
     4801 tctccttgag aacctgtaca taggatcatc gtttcattgg caggcaagcg attgatttcg
     4861 tgtgcatcta caaatgtgcc gtctggaact ttgatatatc ctaaacgttg accgttgacg
     4921 atggcatttt ccatgcttcg tccgaaaaca gcgattttac gaccagttgc cacagcggca
     4981 tctgctgctt gctgcaaacg gaagatgtta gaagcaaagc tggcaaaaat gatacgtcct
     5041 tcgatttttg aaaagatctt caaaatagaa tgcccaatcg ttttttctga tttagtaaat
     5101 gttggaatct cagcatttgt actatcagat aaaaggcaca agactccttc ttccccgata
     5161 cgagccattt tatgcaagtt tgctggttcg ccaacaggag tgaaatcgaa tttgaagtct
     5221 ccagtcgcta cgatatttcc agaaggtgtt ttcaccacga tccctaatgc atctgggata
     5281 ctatgggtcg ttctgaaaaa gctgacagaa gtctttctaa aacggatgat cgtatcttca
     5341 ttgatctcat ggagctctgc atctcgaagc aagccatgct catctaattt gtttgtgatc
     5401 aacgctaatg cgagaggacc agcata
//
LOCUS       DACEEZ010000246         4745 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20313_2017
            SAMN12639966-rid9879223.guided.139, whole genome shotgun sequence.
ACCESSION   DACEEZ010000246 DACEEZ010000000
VERSION     DACEEZ010000246.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639966
            Sequence Read Archive: SRR10023158
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4745)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4745)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741683.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741683.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:44:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,016
            CDSs (total)                      :: 2,962
            Genes (coding)                    :: 2,889
            CDSs (with protein)               :: 2,889
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 36 of 73
            Pseudo Genes (incomplete)         :: 29 of 73
            Pseudo Genes (internal stop)      :: 18 of 73
            Pseudo Genes (multiple problems)  :: 8 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4745
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639966-rid9879223.guided.139"
                     /isolate="E20313_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            <1..341
                     /locus_tag="IWO89_002991"
     CDS             <1..341
                     /locus_tag="IWO89_002991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HAQ0457659.1"
                     /translation="YNQAYESGKMPKEVRDQQLAAIIPYYKSFSREEMNDVVKVDSSL
                     AAMQFMLVARDHGYETNPIGGFEADQLAEAFGLDKDRYVPVIILSIGKAVEEGYESVR
                     LAPEKITMFE"
     gene            695..2173
                     /gene="msr"
                     /locus_tag="IWO89_002992"
     CDS             695..2173
                     /gene="msr"
                     /locus_tag="IWO89_002992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Msr family ABC-F type ribosomal protection
                     protein"
                     /protein_id="HAQ0457660.1"
                     /translation="MENLAVNITNLQVSFGNQLELSIDSLRVYQQDRIGIIGENGVGK
                     STLLKLIAGELFPDHGKIQTEITFNYLPQLTYLAEAKDLNLELASHFQLRLEETSERK
                     WSGGEERKIELIRLLSSYEQGMLLDEPTTHLDRKSIDRLIEELRYYYGTLVFVSHDRY
                     FLDELASKIWEVKDGEIREFSGNYSAYLTQKELEKKTQLREAESIMKEKKRLEKSIQE
                     KKKQAEKLEKVSSKKKKQQIRLDRLSSSKQKDSVQKAIQKNAKTLERRLQKIGETTKP
                     QQMKQIRFPVPKSLELHSRYPIMGQNVQLERSGRTLLVNGDFQFSLGKKIAIVGENGS
                     GKTTLLEHIRKQGEGILLSPKVSFQVYQQKGYQMTSEESIIRFVMRQTEFSESLVRSL
                     LNHLGFAQETLTKPLCTLSGGEATRLTIALLFTKPSNVLLLDEPTNFIDMATIEALEK
                     LMQIYPGTILFTSHDSYFVERTADEVYEIKGQKIKKVLTRNF"
     gene            2220..2666
                     /locus_tag="IWO89_002993"
     CDS             2220..2666
                     /locus_tag="IWO89_002993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2798 domain-containing protein"
                     /protein_id="HAQ0457661.1"
                     /translation="MPTNKKEGIIFTTMMCFLMVLGMSAYNLWLHQDLHLSSLLIGLV
                     PGFVVAFILDVFIIGVLAKKIAFKLPVNPESKVQMILTISCLMVLGMVTCMSLFGVIM
                     EQGIPDQLFSAYMNAWKMNVIAALPLQLIIVGPLSRSVLKAIQSNE"
     gene            complement(2865..3380)
                     /locus_tag="IWO89_002994"
     CDS             complement(2865..3380)
                     /locus_tag="IWO89_002994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HAQ0457662.1"
                     /translation="MNTLAYILLCVLLSKPRSGYELKQLITIFWEAHHSQIYTTLAKL
                     DQQGYVTVLDKDEHSQKKIYHLTDEGRALVEEWIKEETPAPTQKDEFLAKIYAFSTLD
                     KTTATGLLFTREQQLNKIMQKNQKKLNDLSLDKKQEFGRYVVIQRRIMLCQQELQWCQ
                     QVREQMQQFFE"
     gene            3497..4699
                     /locus_tag="IWO89_002995"
     CDS             3497..4699
                     /locus_tag="IWO89_002995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAQ0457663.1"
                     /translation="MEILENQIDIQEQTNEETKGKSSALVVAATCMIAFMGVGLVDPI
                     LKTIAAQLNATPAETTLLFTSYMLVTGVVMLFTGFLSSRIGLKKTLMTGLFIIVVFAG
                     LGGLSGNIGMLIGLRAGWGVGNALFVSTALATIVSILVGNTEKAIMMYEAALGCGMAV
                     GPLVGGVLGSGSWRYPFFGVAFLMFCGFIALAVLLPETEKPKRKVGLWEGVKALSNHK
                     LRSVGLIALLYNFGFFTLLAYAPFLLIGYSELEVGFVFFGWGVLLAIASIFVAPALER
                     KFTTYRTILAAFVLFIICLLLLGAGTSMPVLVPISIVLAGFFQGIINTLLTTIAMEIP
                     GLQRNVASSSYSFVRFFGGALAPFIAGVIGEHIGESYAFYFAGAIVLISVCCIIYHRN
                     YFATNEEE"
BASE COUNT         1437 a          791 c         1031 g         1486 t
ORIGIN      
        1 tctacaacca agcttacgaa tcagggaaga tgccaaaaga agtcagagat caacaattag
       61 ctgcgatcat tccttattac aagagttttt caagagaaga aatgaacgat gtagtcaaag
      121 tcgattctag cttagcggct atgcaattca tgttagttgc gcgtgatcat ggctatgaaa
      181 caaatccaat tggtggattc gaagcagatc agttggcaga ggcatttggt ttggataaag
      241 accgttatgt tccagttatt attttatcaa taggaaaagc ggtagaagaa ggttacgagt
      301 ctgttcgttt agcaccagaa aagatcacta tgttcgaata aaaggttttc agttactatt
      361 cagtagctat tgcttttttt gaatcgtgtg tggtaaccta ataaaaaagg aggtcggaaa
      421 aatgactgca tcgatgaaat tacgtttcga acttttgaat aacaactaaa taaattcaaa
      481 agacatgtat aaacaacaat ttcatggatg gagtatgtcc attttttcga acgacagtag
      541 aataggtgaa agctatgaca gatttggtga tttagtcata gttatttcgt tcagagtagg
      601 aaaagatgtg atgcagtttt tttgcaaaca gatttttctg ctctttttta gtatcgataa
      661 ttgttgtttt acatgaaata ggagtgtaaa acatatggaa aatttagcag taaatataac
      721 aaatctacaa gtcagctttg gcaaccagct agaactatct attgattctc ttcgtgtcta
      781 tcagcaagat cgtataggga tcataggaga aaatggtgtc ggtaaatcga ctttgctcaa
      841 actaatagcc ggtgaacttt ttcccgatca tgggaaaatc caaacagaga tcaccttcaa
      901 ctacctgcct caattaacct atctcgctga agcaaaggac ctaaatttgg aattagccag
      961 tcatttccag ttaagactgg aagaaacttc agagcggaaa tggagcggag gggaagaacg
     1021 aaagatcgag ttgatacgtc ttctttcttc ttatgaacaa gggatgcttc tagacgagcc
     1081 aacaacccat ctagatagaa aaagtattga tcgactgatt gaagagcttc gttattatta
     1141 tggtacgctg gtttttgtta gtcatgatcg ctattttcta gatgagctag catcgaaaat
     1201 ctgggaagta aaagacggag aaatccgaga gttttcgggg aattatagtg cctatctcac
     1261 tcaaaaggaa ttggagaaaa agactcagct acgagaagca gagtcgatca tgaaagagaa
     1321 aaaacgattg gaaaaatcga tccaagaaaa gaaaaaacaa gcggaaaagt tggaaaaagt
     1381 gtccagtaaa aagaaaaagc aacaaatcag actggatcgg ttgtcttcct ctaaacaaaa
     1441 agacagtgta caaaaagcca tccaaaagaa tgcgaaaaca ttagagagaa gactccaaaa
     1501 aataggagaa acaaccaagc cgcagcagat gaaacaaatc cgttttccag taccaaaatc
     1561 tcttgaactc cacagccgct atcccatcat gggacaaaat gtccaattgg aaagaagtgg
     1621 gagaacatta ctggtaaatg gtgattttca gttttcttta ggtaaaaaaa tcgcgattgt
     1681 cggtgaaaat ggttcaggta agacaacctt attagaacat atccgcaaac aaggagaagg
     1741 aatccttctc tctccgaaag taagctttca agtatatcag caaaagggtt atcaaatgac
     1801 atctgaagaa tccatcattc gttttgtcat gagacaaaca gagttttcag aatcgcttgt
     1861 ccgtagtttg ctgaatcact tagggtttgc tcaggaaact ttgacgaaac cgttatgtac
     1921 attaagtggg ggagaagcga cccgtctgac gattgctttg ctttttacta agccaagtaa
     1981 tgtgttgctg ttagatgaac cgactaattt tattgatatg gcaacgatcg aagctttaga
     2041 gaagctaatg caaatatatc cgggaacgat tttgtttact tctcatgatt cttactttgt
     2101 cgagcgtacg gctgatgaag tttatgaaat aaaagggcag aaaataaaaa aagtacttac
     2161 gagaaatttt taaatgtgct acgatgattt tcgtgacaaa tttaaaggag tggtacagat
     2221 tgcctacaaa taaaaaagaa ggaataattt ttacaacaat gatgtgtttt ttgatggtct
     2281 tggggatgag cgcgtataat ttatggctgc atcaagatct tcatttatca agtttgttga
     2341 ttggtctagt gcctggattc gtggtggctt tcattttgga tgtcttcatc atcggtgtgc
     2401 tggcgaaaaa aattgccttt aaactaccag taaatccaga aagcaaggtc caaatgatct
     2461 taactatttc atgcttgatg gttttaggaa tggtgacatg tatgtcttta ttcggtgtga
     2521 tcatggaaca aggaatacct gatcaattat tctctgctta tatgaatgct tggaaaatga
     2581 atgtgattgc cgctttaccg ttgcagttga tcatcgttgg cccgctttca agaagtgtgc
     2641 taaaagcaat tcaaagtaat gaatgatgga acactgttat ttgaaaaaag gccttggcat
     2701 gctttttgtc atagtctctt gaatgcagtt ctagaaaaat agtgatccga acgataggtt
     2761 ttggcctgtc gttcggatcg ctttttcttt tccaactgta ttgctctttt attgaagata
     2821 atttgctctt caatagatat ttctagaaag gttctgttta tcccttactc aaaaaactgc
     2881 tgcatttgtt ctcttacttg ctgacaccat tggagttcct gctgacaaag cataattctg
     2941 cgctggatca caacataacg tccaaattct tgtttcttgt ctagcgagag gtcattcaat
     3001 tttttttgat ttttttgcat gattttgttc agctgttgtt ctctagtgaa tagcagtcca
     3061 gtagctgttg ttttatcgag cgtcgaaaaa gcatagattt ttgctagaaa ttcatctttt
     3121 tgcgtaggag cgggagtttc ctctttgatc cattcctcta caagagcacg tccttcatca
     3181 gtcaagtggt agatcttttt ttgagagtgc tcgtctttgt ccaaaacggt cacataaccc
     3241 tgctggtcta acttggcaag cgttgtatag atctgactgt gatgggcttc ccaaaaaatt
     3301 gtgatcagct gcttcaattc atacccagaa cgtggtttgg ataaaagcac gcataataat
     3361 atataggcta aagtattcat aaaaatccta cctctcgtct tattttcttt attatatcac
     3421 agtaaagttt ccacgtttta catgtgcata ttgacatgta aaataaatca tgtatgatgc
     3481 taatagagag aaagggatgg aaatattgga gaatcaaata gatatacaag aacaaacaaa
     3541 tgaagaaaca aaggggaaaa gttcagcgct agttgtagca gctacctgta tgatcgcttt
     3601 tatgggagtg ggtctagtag acccaatctt aaaaacgatt gctgcacagt taaacgctac
     3661 gccagctgaa acgaccctgt tatttactag ttacatgtta gtcactggtg tggtgatgct
     3721 atttacaggt ttcttatcta gtcggatcgg tttgaaaaaa acattgatga caggattgtt
     3781 tatcatcgtt gtattcgctg gtcttggcgg attatctggg aatatcggaa tgctgattgg
     3841 gttacgtgcc ggctggggtg tcgggaatgc tttatttgtc tctactgctt tagcaacgat
     3901 cgtcagtatt ttagttggta acacagaaaa agcaatcatg atgtatgaag cagcacttgg
     3961 ctgcggaatg gctgttggcc ctctagtcgg tggggtcttg ggcagtggtt catggcgtta
     4021 tccatttttt ggggtagcat ttttgatgtt ttgcggtttt atagccctag cggtactttt
     4081 acctgaaact gagaaaccaa agagaaaagt aggcctttgg gaaggtgtca aagcgttaag
     4141 caatcataaa cttcgttcag ttggactaat cgcattgtta tataatttcg gattctttac
     4201 tttacttgcg tatgcgccat ttttattgat tggctattct gaattagaag tcggttttgt
     4261 attttttggc tggggtgtgc ttctggctat tgcttcgatt ttcgttgcac cggctcttga
     4321 gcggaagttt acgacttatc ggacaatttt agcagcattt gtcttattca ttatttgttt
     4381 gctgttatta ggagctggaa cttctatgcc agtccttgta ccgatcagca ttgtgttagc
     4441 aggatttttc caaggaatta tcaatacact gttgacgacg attgctatgg aaataccagg
     4501 tctgcaacga aacgttgctt cgtccagcta tagttttgtc cgctttttcg gtggagcttt
     4561 agcaccattt attgcaggtg tgattggtga acatattggt gaaagctatg cgttttattt
     4621 tgcaggtgcc attgttctta tcagtgtatg ttgtatcatt tatcaccgta attattttgc
     4681 tacaaatgaa gaagaataag aaatcagagg ttgtgaaaaa gctgtcctgc atcaagtcat
     4741 aagaa
//
LOCUS       DACEEZ010000247         4493 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20313_2017
            SAMN12639966-rid9879223.guided.143, whole genome shotgun sequence.
ACCESSION   DACEEZ010000247 DACEEZ010000000
VERSION     DACEEZ010000247.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639966
            Sequence Read Archive: SRR10023158
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4493)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4493)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741683.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741683.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:44:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,016
            CDSs (total)                      :: 2,962
            Genes (coding)                    :: 2,889
            CDSs (with protein)               :: 2,889
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 36 of 73
            Pseudo Genes (incomplete)         :: 29 of 73
            Pseudo Genes (internal stop)      :: 18 of 73
            Pseudo Genes (multiple problems)  :: 8 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4493
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639966-rid9879223.guided.143"
                     /isolate="E20313_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            <1..672
                     /locus_tag="IWO89_002996"
     CDS             <1..672
                     /locus_tag="IWO89_002996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:proton antiporter"
                     /protein_id="HAQ0457664.1"
                     /translation="TDDEKQEVQEIQALILSIERDGLDESYRSGKLSSNGYRFYSRFL
                     SRFEHSITSQILSFIGFWFIVVRRLMRIVLHPKMFWQRRYADRQTFISDEDIQEIRET
                     YQKNTQLIIESLDNLTDIYDETMVQFFIQQRKLEGIKMNSGNLISSWMIQQDSLFTKK
                     MLRGYYLERKIIDEYEVAEKITTFSANSYRRNINLLESYTMNKPSDNFSFRFAFRAKS
                     KKRVQ"
     gene            837..2723
                     /locus_tag="IWO89_002997"
     CDS             837..2723
                     /locus_tag="IWO89_002997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HAQ0457665.1"
                     /translation="MLKELKVTDLKKTYGEKDLFDQISFLVHEKDRIGLIGTNGTGKT
                     SLLNIIAGIDSSDGDRQTVFYPTDYRIGYLSQAAEFSDELTVLQAVFQGNSPLIQTVR
                     AYEEALIELGENGEDPDVQKRYAKAEEQMNKEDAWTTDTNAKIILQKLGISELDKKIS
                     TLSGGQKKRVSLAQVLIDEPDLLLLDEPTNHLDYEAIEWLENYLKQYRGALLMVTHDR
                     YFLDRVANRIFELSFGKLYEYKGNYEAYVLEKAERDRVEVEQEEKRKRLYKQELAWMR
                     AGVQARGTKQQARINRFEDLKENLYQVNQEDDLELNLATQRLGKKVLEIKDGSYRIND
                     QTLLEHLDLLIQSRERLGITGKNGAGKSTLLNILAGRIPLDSGTMSIGETVHLAYYTQ
                     ENEEMAPDKRMIAYLQEAAEEAKTADGSQIGVAELLERFLFPRFMHGTLIGKLSGGEK
                     RRLFLLKLLIQQPNVLLLDEPTNDLDIATLTILEDYFRSFPGAVITVSHDRYFLDKVA
                     DKLLVFQGNGKQELYYGNMSSYLLKQKETQQPAEKAKPKIQSKEPAEKKKLSYMEQKE
                     WETIEDEIAELEEKISLLQEEMNHQGDNFTRLQELQNDVSETEAQLEEKMARWEYLSE
                     WVED"
     gene            2735..3682
                     /locus_tag="IWO89_002998"
     CDS             2735..3682
                     /locus_tag="IWO89_002998"
                     /EC_number="2.1.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HAQ0457666.1"
                     /translation="MEQAYLDLGRKLLEEGHRKEDRTGTGTLSLFGYQMRFDLQKGFP
                     LLTTKRVPFGLIKSELLWFLKGDTNIRYLLQNKNHIWDEWAFERYIKSPDYNGPDMTD
                     FGHRCLTDPEFNEEYKKQSKLFCEKILTDDSFAETYGDLGHIYGYQWRHWETKDGGFI
                     DQIKQVIEAIKKTPDSRRLIVSAWNPEDVPSMALPPCHTMFQFYVQEGRLSCQLYQRS
                     GDVFLGVPFNIASYALLTHLIAHETGLAVGEFVHTLGDAHLYINHIEQMKEQLSREIR
                     AFPTLVLNQEKQSVFDFEMEDIQLEGYDPHPAIKAPIAV"
     gene            3702..4229
                     /locus_tag="IWO89_002999"
     CDS             3702..4229
                     /locus_tag="IWO89_002999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HAQ0457667.1"
                     /translation="MLAAIWAQDENGLIGKEDRLPWRLPNDLKFFKQMTEANTLVMGR
                     KTFEGMGSRPLPNRQTIVLTRDSSYKAEGVHVMHSVEDVLALEKETDGIFFIAGGSAV
                     YQEFLPFCTILYRTVIHHVFDGDAYFPPVDWSDWSLINTSQGEMDEKNRYPHQFETYQ
                     RNENPVKSGTGELTI"
     gene            complement(4292..>4493)
                     /locus_tag="IWO89_003000"
     CDS             complement(4292..>4493)
                     /locus_tag="IWO89_003000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hemolysin III family protein"
                     /protein_id="HAQ0457668.1"
                     /translation="LCVVAAVPLYHSLGMTGVILMVAGGLSYTIGAFFYSLNSIRYMH
                     VVWHLFVMLGAGFMYFSVLFFT"
BASE COUNT         1579 a          762 c          961 g         1191 t
ORIGIN      
        1 accgatgatg aaaagcaaga agtacaagaa atacaagcac tgattttgtc gatcgaacgg
       61 gatggattgg atgaaagtta tcgttcgggc aagctttctt ctaatggtta tcgtttttac
      121 tcccgttttc tttctcgatt cgaacattct atcacaagcc agattttgtc ttttatcggc
      181 ttttggttca ttgttgtccg taggttaatg cgaatcgttc tccatccgaa aatgttttgg
      241 caaagaagat atgctgatcg tcagacattt attagtgatg aagatatcca agaaatcaga
      301 gagacatatc aaaaaaatac tcagttgatt attgaaagtt tagataactt gactgatatt
      361 tatgatgaaa cgatggttca attttttatc cagcaacgca aacttgaagg aataaaaatg
      421 aactcgggga atttgatttc ttcgtggatg atccagcaag atagtctgtt tacgaaaaaa
      481 atgctaagag gttattatct tgaaagaaaa atcattgatg aatacgaagt tgctgaaaaa
      541 atcacgacgt tttctgcgaa cagctatcgc aggaatatca atttgttaga atcttatacg
      601 atgaacaaac catctgataa cttttctttc cgatttgcgt ttcgagcaaa gtcaaaaaaa
      661 cgagtccaat aaaataaaaa ggtaaaaatt ttcaagtttt ataaaaaaca aaacatatca
      721 gaacaggatc gaatcggtcc tgttcttttt ttgagaacac aaaaagaaca aataccaaaa
      781 aaattttcct gaagttcact atttttatga tacaatatcc aagataagga gggaacatgt
      841 tgaaagaatt aaaagtaact gacctcaaaa aaacttatgg tgaaaaagat ttgtttgacc
      901 agatttcttt tttggtccac gaaaaagacc gtatcgggtt gattgggacg aacgggacgg
      961 gtaaaacaag tctcttaaat attattgccg gtatcgatag cagcgacggt gatcggcaaa
     1021 cagtgtttta tccaactgat taccgtattg gttatctttc tcaagcagct gaattttccg
     1081 atgaattaac tgttctccaa gcagtctttc aaggaaacag ccccttgatc caaactgttc
     1141 gagcatatga ggaagcattg atcgaactag gggaaaacgg agaagaccct gatgtgcaaa
     1201 aacgttatgc taaagcagaa gaacaaatga ataaagaaga tgcttggact acagatacca
     1261 acgcaaagat tattttgcaa aaattaggaa tcagtgaatt agataaaaaa atcagtactt
     1321 tatcaggtgg tcagaaaaaa cgagttagtc tggcgcaagt gttgattgat gaacccgatt
     1381 tactattact agacgaacca acaaaccatt tagattatga agcgatcgag tggttggaaa
     1441 actatttgaa acaataccgt ggcgcactat tgatggttac ccacgatcgt tattttctag
     1501 atagagtagc aaaccgaatc ttcgaacttt catttggaaa attatacgag tataaaggaa
     1561 attatgaagc ttacgtctta gaaaaagcag aacgtgatcg tgtagaagta gaacaagaag
     1621 aaaagcgcaa gcggctttat aaacaggaat tagcttggat gagggctggc gtacaggcac
     1681 gtggcaccaa gcagcaagca cggatcaacc gtttcgaaga tctaaaggag aacttgtacc
     1741 aagtaaacca agaagatgat ttagagctga acctagcgac acaacgttta gggaaaaaag
     1801 tactggaaat caaagatggc agttaccgta taaatgacca aacactgcta gaacatttag
     1861 atttattgat tcaatctcgg gaaagactag gtattactgg taaaaatggt gcgggaaaat
     1921 cgacattgct taatattcta gctggcagaa ttcccctgga tagcggtaca atgtctatcg
     1981 gtgaaacggt tcatcttgcg tattatacac aagaaaatga agaaatggca ccagataaac
     2041 ggatgattgc ttacttacaa gaagctgctg aagaagcaaa aacagcagat ggcagtcaaa
     2101 tcggagtagc agaattactt gaacgatttt tatttccaag atttatgcat gggacactga
     2161 taggcaaact ttcaggcgga gaaaaacgcc ggttattttt gttgaaactg ctgattcagc
     2221 aacctaatgt tttattattg gatgaaccga ccaatgattt ggacatagca acattgacga
     2281 ttttggaaga ttattttaga agttttccag gtgctgtgat cactgtttcc catgaccgtt
     2341 atttcttgga caaggtagca gataagttat tagtattcca aggaaacgga aaacaagaac
     2401 tttattacgg aaacatgtcc agttatttac tgaagcaaaa agagacccag cagcctgctg
     2461 aaaaagcgaa accaaaaata cagagcaagg aacctgctga aaagaagaaa ttatcttaca
     2521 tggaacaaaa agaatgggaa acgattgaag atgagattgc agaacttgaa gaaaaaatct
     2581 cattgctcca agaagagatg aatcatcaag gagataattt cactcgatta caagaattgc
     2641 aaaacgacgt atctgaaaca gaagcacaac ttgaagaaaa aatggcacgg tgggaatact
     2701 tgagtgaatg ggtagaagat taggaggaaa aaggatggaa caagcgtatc ttgacttagg
     2761 aagaaaattg ttggaagaag ggcatcgtaa agaagacaga acaggcacag gcacattaag
     2821 cttattcgga taccaaatgc ggtttgatct gcaaaaaggg tttcctttgt taacaacgaa
     2881 acgagtacct tttggattga tcaaaagcga attgctttgg tttttaaaag gagatacgaa
     2941 tatccgctat ctattacaaa ataaaaacca tatttgggat gaatgggcat ttgaacgcta
     3001 catcaaaagt ccagactata atggaccaga tatgactgat tttggccata gatgtttgac
     3061 agatcctgaa ttcaatgaag aatataaaaa gcagagcaag cttttttgcg agaagatcct
     3121 aaccgatgat tcctttgctg aaacatacgg cgatcttgga cacatctacg gctatcaatg
     3181 gcgtcactgg gaaacaaaag acggtggctt tatcgatcaa atcaaacaag tgatcgaagc
     3241 aatcaaaaag actcctgatt ctagaagatt gatcgtttct gcatggaatc cggaagatgt
     3301 tcctagcatg gctttgcctc cttgtcatac gatgtttcaa ttttatgttc aagagggacg
     3361 gctaagctgc caattgtatc aacgcagtgg ggatgtgttt ctaggcgttc cgttcaacat
     3421 tgcaagttat gcgttgctga ctcacctaat tgcacatgaa acaggtttag cggttggcga
     3481 atttgttcac acattaggag atgctcatct ttatatcaat catatcgaac aaatgaaaga
     3541 acaattgagc cgagaaatac gcgcatttcc gactttagtt ttgaatcagg aaaagcagtc
     3601 agtatttgat tttgagatgg aagatatcca attagaagga tatgacccac atccggcgat
     3661 caaagctcct atcgctgttt agaatcagaa aggatgagaa aatgttagct gcaatttggg
     3721 cacaggatga aaacggattg attggaaaag aagaccgtct tccttggcgt ttgccaaatg
     3781 atctgaaatt cttcaaacaa atgacagaag caaatacact agtgatggga cgaaaaacat
     3841 tcgaagggat gggtagccgt ccgttgccga atcgtcagac gattgttttg acgagagatt
     3901 cttcctataa agcagaaggc gtgcatgtca tgcatagtgt ggaagacgtg cttgctctgg
     3961 aaaaagaaac agacggtatc tttttcatcg ctggagggtc agctgtttat caagaatttc
     4021 tgccattttg tacgatatta taccgtacag tcatccatca tgtattcgat ggagatgctt
     4081 attttccacc tgttgactgg tctgattggt cactgatcaa tactagtcaa ggagaaatgg
     4141 atgaaaaaaa tcgttatccg catcaatttg aaacgtatca gcgtaatgag aatcctgtaa
     4201 aaagcgggac aggcgaattg acgatataaa aaagctatgg atagactagc tgttatttag
     4261 agctgtccca tccatagctt tatttttttt attaagtaaa gaataaaaca gagaagtaca
     4321 taaaacctgc acctaacata acaaacaaat gccaaacaac atgcatatag cggatagaat
     4381 tcaagctata aaagaatgcg cctatcgtgt aagaaagccc gcctgctacc atcaaaataa
     4441 caccagtcat tccaagagag tgataaagag gaacagcagc cacgacacag agc
//
LOCUS       DACEEZ010000248         3447 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20313_2017
            SAMN12639966-rid9879223.guided.163, whole genome shotgun sequence.
ACCESSION   DACEEZ010000248 DACEEZ010000000
VERSION     DACEEZ010000248.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639966
            Sequence Read Archive: SRR10023158
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3447)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3447)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741683.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741683.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:44:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,016
            CDSs (total)                      :: 2,962
            Genes (coding)                    :: 2,889
            CDSs (with protein)               :: 2,889
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 36 of 73
            Pseudo Genes (incomplete)         :: 29 of 73
            Pseudo Genes (internal stop)      :: 18 of 73
            Pseudo Genes (multiple problems)  :: 8 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3447
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639966-rid9879223.guided.163"
                     /isolate="E20313_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            9..1049
                     /locus_tag="IWO89_003001"
     CDS             9..1049
                     /locus_tag="IWO89_003001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000288739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HAQ0457669.1"
                     /translation="MYTSINGELCKIDRAYSGEIVILQNEFLKLNSVLGDTKLLPQRK
                     KIENPHPLLQTTVEPSKPEQREMLLDALLEISDSDPLLRYYVDSTTHEIILSFLGKVQ
                     MEVISALLQEKYHVEIELKEPTVIYMERPLKNAEYTIHIEVPPNPFWASIGLSVSPLP
                     LGSGMQYESSVSLGYLNQSFQNAVMEGIRYGCEQGLYGWNVTDCKICFKYGLYYSPVS
                     TPADFRMLTPIVLEQAFRKAGTELLEPYLSFKVYAPQEYLSRAYNDAPKYCANIVNTQ
                     LKNNEVIIIGEIPARCIQDYRNDLTFFTNGLSVCLAELKGYQVTTGEPVCQTRRLNSR
                     IDKVRYMFNKIT"
     gene            1147..1335
                     /locus_tag="IWO89_003002"
     CDS             1147..1335
                     /locus_tag="IWO89_003002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011988843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HAQ0457670.1"
                     /translation="MINYKWILCPICGNKTRLRIRVDTILKNFPLYCPKCKNETLINV
                     QKMNIITIKEPDAKTQSR"
     gene            complement(1393..1746)
                     /locus_tag="IWO89_003003"
     CDS             complement(1393..1746)
                     /locus_tag="IWO89_003003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001227349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAQ0457671.1"
                     /translation="MRKKEDKYDFRAVGLAIKEARMKRGLTREQVGTMIEIDPRYLTN
                     IENKGQHPSTQVLYDLVSLLHVSIDEFFLPTDNLIKSTRRLQVEKYMDSFTDKELSLM
                     EALAKGINEARNIEG"
     gene            2277..2699
                     /locus_tag="IWO89_003004"
     CDS             2277..2699
                     /locus_tag="IWO89_003004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000804881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HAQ0457672.1"
                     /translation="MKPSSFQETIENQFDYICKQVIEDERKDYFKHLDRLSAKEVSFS
                     DIDNYLVNSFSTVDTYVTDFQLFKLFGLRINVESDLLSEALRHLSEKKRNIILLHYFM
                     DMSDVEIAELLNLNRSTVYRHRISGLAMIKEFMKECKE"
     gene            2696..2926
                     /locus_tag="IWO89_003005"
     CDS             2696..2926
                     /locus_tag="IWO89_003005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002411750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAQ0457673.1"
                     /translation="MKTAYPRVPFPLIVKATDGDVEAINQIVKHYRGYTSKRSLRRMT
                     DEYGNSHMVIDETLRGRMETKLISKILSFEIK"
BASE COUNT         1131 a          569 c          645 g         1102 t
ORIGIN      
        1 ttacagaaat gtatacttca ataaatggtg aattatgtaa gattgataga gcttattctg
       61 gagaaattgt tattttgcaa aatgagtttt tgaagttaaa tagtgttctt ggagatacaa
      121 aactattgcc acagagaaaa aagattgaaa atccgcaccc tctactacaa acaactgttg
      181 aaccgagtaa acctgaacag agagaaatgt tgcttgatgc ccttttggaa atctcagata
      241 gtgatccgct tctacgatat tacgtggatt ctacgacaca tgaaattata ctttctttct
      301 tagggaaagt acaaatggaa gtgattagtg cactgttgca agaaaagtat catgtggaga
      361 tagaactaaa agagcctaca gtcatttata tggagagacc gttaaaaaat gcagaatata
      421 ccattcacat cgaagtgccg ccaaatcctt tctgggcttc cattggttta tctgtatcac
      481 cgcttccgtt gggaagtgga atgcagtatg agagctcggt ttctcttgga tacttaaatc
      541 aatcatttca aaatgcagtt atggaaggga tacgctatgg ttgcgaacaa ggattatatg
      601 gttggaatgt gacggattgt aaaatctgtt ttaagtacgg tttatactat agccctgtta
      661 gtactccagc agattttcgg atgcttactc ctattgtact ggagcaagcc tttagaaaag
      721 ctggaacaga attgttagag ccatatctta gttttaaagt ttatgcacca caggaatatc
      781 tttcacgggc atataacgat gctcccaaat attgtgcaaa tatcgtaaat actcaactga
      841 aaaataatga ggtcattatt attggagaaa ttcctgctcg atgtattcaa gattatcgca
      901 atgatttaac tttttttaca aatgggctta gtgtttgttt agcagagcta aaaggatatc
      961 aggttaccac tggcgaacct gtttgccaga cccgtcgtct aaatagtcgg atagataaag
     1021 taagatatat gttcaataaa ataacttagt gcgttttatg ttgttatata aatatggttt
     1081 cttattaaat aagatgaaat attctttaat atagatttga attaaagtgg aaaggaggag
     1141 attgttatta taaactacaa gtggatattg tgtcctattt gtggaaataa aacaagacta
     1201 cgaatacgag tggatactat acttaaaaat ttccctttat actgccccaa atgtaagaac
     1261 gaaactttaa ttaatgttca aaaaatgaat ataataacaa tcaaagagcc agacgccaag
     1321 acgcagagcc gataatttga gaaatgaaac tctcatctta tcggctcttt ttgtttatct
     1381 gaattttact gactagcctt caatatttct tgcttcattg ataccttttg ctagtgcttc
     1441 cattaaggat agttctttgt ctgtaaagct atccatgtat ttttctacct gtaaccttcg
     1501 agtgctttta atcaaattat ctgtaggtaa gaaaaattca tcaatagata catggagtaa
     1561 tgatacaaga tcatacagca cttgtgtact tgggtgttgt cctttgtttt ctatatttgt
     1621 taaataacgt gggtcaattt caatcattgt tcccacttgt tcacgagtga gaccccgttt
     1681 cattcgagcc tctttaatcg ctaaaccaac ggctctaaaa tcgtatttat cttctttttt
     1741 acgcatacta aatcacctct atacatttta ctgttcctat ataaccataa acaggtatag
     1801 aaaaacatgt tatatagttt atgagttcct atagaacact gtggaattta ttaatgcgat
     1861 gaatccaatt tcaaatagaa aggtcgctga ttttatttac ctatacaact ttataaaatt
     1921 tagtcatact cctagaaagt agataaaatt ggcgacttaa tggagggaaa agtcgccatg
     1981 agaaagataa tctgtcatct agttctctta attaagttct tatcaaaaaa acttatccac
     2041 ctgtactgga tagatatgac catcaatgtg gttttgcaga ttacatagat agaaaaatca
     2101 aagcatatga acagagaaaa caatctgttt atgtgctttt ttggtcactt aataaatatc
     2161 ttttaggatt tctcttcatt tatgcaacaa atcccccgtt cgtgtcgggg tttagtatga
     2221 agggagataa agaagcacat gccactccct ttcacgacag aaagggggtg aaaagcatga
     2281 aaccatcttc ttttcaagaa accatagaaa atcagtttga ctatatctgc aaacaagtta
     2341 ttgaagatga acgaaaagat tacttcaaac atctagacag actatcagca aaagaagtct
     2401 cgttttctga tatagataac taccttgtca actctttttc tactgttgat acgtacgtca
     2461 ctgatttcca actatttaaa ttatttggat taagaatcaa tgttgaaagt gatttattaa
     2521 gtgaagccct gcgtcatctt tcagagaaaa aacggaacat tattttactt cattacttta
     2581 tggatatgag tgatgtggag attgcggagt tactaaattt gaatcgttcg actgtttatc
     2641 gtcatcgaat aagtggacta gccatgatta aagaattcat gaaggagtgt aaagaatgaa
     2701 aacagcttat ccaagggttc ccttccccct cattgtaaag gcgacagatg gcgacgtgga
     2761 agcaattaat cagattgtta aacattatcg agggtatacc tcaaaacgtt cattacgtcg
     2821 catgacagac gaatatggaa attctcatat ggttattgat gaaaccctac gtggcagaat
     2881 ggaaacgaaa ctcatctcta agattttatc gttcgaaatt aaatagtccc attttttcgt
     2941 ggaagcgtgt cgaatcgtat ccacgcttcc cgatttggga ggtttgttat tccatcaaag
     3001 catattgtgt gttcagtagg ttttgatagg ctaacaagcc aaacgttctt tgacaaccaa
     3061 ataaaggcaa tcgaatacgt ttcggtaaga aaagagctga tgaactaaca cgccatgacc
     3121 tacaaaatta gtgagcgatt catgttagta attcgaaagt aatctttagc aggattgctg
     3181 ccatgacttt cttatcgtga taatgatact cccgtatagc catagtccga gcgttagaag
     3241 cgtcacaggc aatgggtacg gctacatgag aaccatgcag aagtgaaatt cctgtgagct
     3301 ttgctagagc tgttcgattg ctaagatatt cttctcttgt cagtgtatta ttataaaact
     3361 atttgatata attataggtg aatattggag ttttcctagc ctataattgg gaaaggagta
     3421 ataatgataa cggtcacaaa aaaggat
//
LOCUS       DACEEZ010000249         2256 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20313_2017
            SAMN12639966-rid9879223.guided.176, whole genome shotgun sequence.
ACCESSION   DACEEZ010000249 DACEEZ010000000
VERSION     DACEEZ010000249.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639966
            Sequence Read Archive: SRR10023158
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2256)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2256)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741683.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741683.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:44:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,016
            CDSs (total)                      :: 2,962
            Genes (coding)                    :: 2,889
            CDSs (with protein)               :: 2,889
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 36 of 73
            Pseudo Genes (incomplete)         :: 29 of 73
            Pseudo Genes (internal stop)      :: 18 of 73
            Pseudo Genes (multiple problems)  :: 8 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2256
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639966-rid9879223.guided.176"
                     /isolate="E20313_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(41..1027)
                     /locus_tag="IWO89_003006"
                     /pseudo
     CDS             complement(41..1027)
                     /locus_tag="IWO89_003006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006701269.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS256-like element IS1542 family transposase"
     gene            1179..1874
                     /gene="vanR"
                     /locus_tag="IWO89_003007"
     CDS             1179..1874
                     /gene="vanR"
                     /locus_tag="IWO89_003007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001280781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanA-type vancomycin resistance DNA-binding
                     response regulator VanR"
                     /protein_id="HAQ0457674.1"
                     /translation="MSDKILIVDDEHEIADLVELYLKNENYTVFKYYTAKEALECIDK
                     SEIDLAILDIMLPGTSGLTICQKIRDKHTYPIIMLTGKDTEVDKITGLTIGADDYITK
                     PFRPLELIARVKAQLRRYKKFSGVKEQNENVIVHSGLVINVNTHECYLNEKQLSLTPT
                     EFSILRILCENKGNVVSSELLFHEIWGDEYFSKSNNTITVHIRHLREKMNDTIDNPKY
                     IKTVWGVGYKIEK"
     gene            1852..>2256
                     /locus_tag="IWO89_003008"
     CDS             1852..>2256
                     /locus_tag="IWO89_003008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanA-type vancomycin resistance histidine kinase
                     VanS"
                     /protein_id="HAQ0457675.1"
                     /translation="MVIKLKNKKNDYSKLERKLYMYIVAIVVVAIVFVLYIRSMIRGK
                     LGDWILSILENKYDLNHLDAMKLYQYSIRNNIDIFIYVAIVISILILCRVMLSKFAKY
                     FDEINTGIDVLIQNEDKQIELSAEMDVMEQKLN"
BASE COUNT          692 a          434 c          443 g          687 t
ORIGIN      
        1 gggattttat tctttattta aaactttgca acagaaccct atttccgata accgtgtatt
       61 gaaaggcatc ttcgaagcca ttatccaata tttcgcaggc ttttgtatat ttaggctggt
      121 cgatatattc accgactaag gcattcttag ctgttcgagc gagttcaata tccgtaaact
      181 taaagatcgc ttttactgct tctctaaacg gttttgaatt cttttttgga atggaactga
      241 agatgtttct taaaaaatgg acctggcatc tctgccaact tgcgttggta aatgacttac
      301 gaatcgcaga cactaggcct ttatgggcat cagaaatgat gagttctgtc ccctttaggc
      361 cgcgttcttt taagtattca aagaagatgg accatgtgtc atcactttct tcattttgaa
      421 tcatgaagcc aatgatttca cggtcgccac cttctgttat cccgatcgca atatggcagc
      481 ttttagaaag cactcgatgg tcctcacgga cttttatgta gagaacatca gtcatcagat
      541 aaggataatt cgtacctgag agtgaacggt tctgccattc gttgaccatc gggtctaact
      601 gctcagtcag gctagaaaca aaagatttcg atacagattt tccacatagc tcttcaacaa
      661 tctttgaaac tttacgtgtc gaaacgcccg aaacatacat ctcaagcatt gaagcgagca
      721 gtgccttttc atttcgctga taacgctcaa acaccgtcgg tgaaaattca ccatcacgtg
      781 ttctaggcac ttttaattcg agtgtaccca cacgagtcgt aaagtctcgc tcataatagc
      841 catttctttg actctgacga ctttctgaac gttcatagtc atcggcttga atatattctg
      901 ttcgttgatt ttccatcaat tggttgaaaa cagtggttaa aatattttta gaaacatcat
      961 cttttaccga atgttcaata atactttgaa cctcttcgtt gttcagtgta aaatgtactt
     1021 gggtcatgta acgtcctcct gggtatgttt ttgtggttaa aaacattgta ccgtaaaagg
     1081 gctgttacat ggccttttat cttttacaca attatatgga ctttatccta taatctgata
     1141 aattattaag ggcaaactta tgtgaaaggg tgataactat gagcgataaa atacttattg
     1201 tggatgatga acatgaaatt gccgatttgg ttgaattata cttaaaaaac gagaattata
     1261 cggttttcaa atactatacc gccaaagaag cattggaatg tatagacaag tctgagattg
     1321 accttgccat attggacatc atgcttcccg gcacaagcgg ccttactatc tgtcaaaaaa
     1381 taagggacaa gcacacctat ccgattatca tgctgaccgg gaaagataca gaggtagata
     1441 aaattacagg gttaacaatc ggcgcggatg attatataac gaagcccttt cgcccactgg
     1501 agttaattgc tcgggtaaag gcccagttgc gccgatacaa aaaattcagt ggagtaaagg
     1561 agcagaacga aaatgttatc gtccactccg gccttgtcat taatgttaac acccatgagt
     1621 gttatctgaa cgagaagcag ttatccctta ctcccaccga gttttcaata ctgcgaatcc
     1681 tctgtgaaaa caaggggaat gtggttagct ccgagctgct atttcatgag atatggggcg
     1741 acgaatattt cagcaagagc aacaacacca tcaccgtgca tatccggcat ttgcgcgaaa
     1801 aaatgaacga caccattgat aatccgaaat atataaaaac ggtatggggg gttggttata
     1861 aaattgaaaa ataaaaaaaa cgactattcc aaactagaac gaaaacttta catgtatatc
     1921 gttgcaattg ttgtggtagc aattgtattc gtgttgtata ttcgttcaat gatccgaggg
     1981 aaacttgggg attggatctt aagtattttg gaaaacaaat atgacttaaa tcacctggac
     2041 gcgatgaaat tatatcaata ttccatacgg aacaatatag atatctttat ttatgtggcg
     2101 attgtcatta gtattcttat tctatgtcgc gtcatgcttt caaaattcgc aaaatacttt
     2161 gacgagataa ataccggcat tgatgtactt attcagaacg aagataaaca aattgagctt
     2221 tctgcggaaa tggatgttat ggaacaaaag ctcaac
//
LOCUS       DACEEZ010000250         2232 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20313_2017
            SAMN12639966-rid9879223.guided.177, whole genome shotgun sequence.
ACCESSION   DACEEZ010000250 DACEEZ010000000
VERSION     DACEEZ010000250.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639966
            Sequence Read Archive: SRR10023158
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2232)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2232)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741683.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741683.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:44:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,016
            CDSs (total)                      :: 2,962
            Genes (coding)                    :: 2,889
            CDSs (with protein)               :: 2,889
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 36 of 73
            Pseudo Genes (incomplete)         :: 29 of 73
            Pseudo Genes (internal stop)      :: 18 of 73
            Pseudo Genes (multiple problems)  :: 8 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2232
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639966-rid9879223.guided.177"
                     /isolate="E20313_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(<1..164)
                     /locus_tag="IWO89_003009"
     CDS             complement(<1..164)
                     /locus_tag="IWO89_003009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HAQ0457676.1"
                     /translation="MKYILSFCTPKLNYLLAYIRLYSGVLHLRDSVRVSEKEKIKVTE
                     MYTSINGELC"
     gene            394..891
                     /gene="dfrG"
                     /locus_tag="IWO89_003010"
     CDS             394..891
                     /gene="dfrG"
                     /locus_tag="IWO89_003010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000868795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trimethoprim-resistant dihydrofolate reductase
                     DfrG"
                     /protein_id="HAQ0457677.1"
                     /translation="MKVSLIAAMDKNRVIGKENDIPWRIPKDWEYVKNTTKGHPIILG
                     RKNLESIGRALPDRRNIILTRDKGFTFNGCEIVHSIEDVFELCKNEEEIFIFGGEQIY
                     NLFFPYVEKMYITKIHHEFEGDTFFPEVNYEEWNEVFAQKGIKNDKNPYNYYFHVYER
                     KNLLS"
     gene            complement(963..>2232)
                     /locus_tag="IWO89_003011"
     CDS             complement(963..>2232)
                     /locus_tag="IWO89_003011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017431446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="insertion element protein"
                     /protein_id="HAQ0457678.1"
                     /translation="VSRTCDILGIGVGTYYHKLEWLYRRCLEFLERYETQPLQTKKFN
                     EMWLNTDKMHYYLNNVRKKGQGSKKYTGFEDLNMQTYIVVSAEVLSRYVFRSDVAYDW
                     NISMDELNEDTRKFKEDHLNTFSRKNDRLDWSYYPQEPSANDSENRNAYLHELGKITN
                     RSRFVDGLNVDAPYTTTAHYWLIKQMVNADEWRMISDDDFSIRNAFYRVFTKELRLSD
                     AHHFICQVNKTKSRKQCLKEFGQAKAELLDWGDIRGFKTKFLRTLASHYLTELLTSHQ
                     FHEEAISKDGERYRKYADNPIKHPLATKDKGFYSVDCRTDLSALEPNEIAKMLLNVND
                     HSTNSFIQQIRRYISSLERPLTTARGDKKSYIYANFNPKYAQFAITILRTYYNFCRPF
                     KSADKKVLTPAQRLGITDKQFDWKDIIYFK"
BASE COUNT          730 a          352 c          385 g          765 t
ORIGIN      
        1 ttacataatt caccatttat tgaagtatac atttctgtaa cttttatttt ttctttttct
       61 gatactctaa ccgaatctcg taaatgtagt actccactat aaaggcgtat atatgcaagt
      121 aagtagttca gttttggagt acaaaatgac aaaatgtact tcaaaatgga ctactttttt
      181 cattttaact tacataccaa attacataca aaatgattta taaaatgcaa ttgaaaatga
      241 taattgaatg aaaatttgtg attttgtggt attatgataa tataacttat agaaaaaatt
      301 gcttattcaa ctaacgggca ggataatgag aaaaggtttt ttgatagaaa taagaaatta
      361 gatggtaaat tagttaaaaa ggctgtgtta taaatgaaag tttctttgat tgctgcgatg
      421 gataagaata gagtgattgg caaagagaat gacattcctt ggaggattcc caaggactgg
      481 gaatatgtta aaaatactac aaagggacat ccgataatat taggtaggaa gaaccttgaa
      541 tcaatcggaa gagccttacc tgacagaaga aatattattc tgacgagaga taaggggttt
      601 acctttaatg gttgtgaaat tgttcattca atagaagatg tttttgagtt atgtaaaaac
      661 gaagaagaaa tttttatttt cggaggagaa cagatttata atttgttttt cccttatgtt
      721 gagaaaatgt acatcacaaa aatacatcat gaattcgaag gagatacttt ttttccagaa
      781 gtgaattatg aggaatggaa tgaggtattt gcccaaaaag ggataaagaa tgataaaaat
      841 ccgtataact actattttca tgtatatgaa agaaaaaact tattgagtta acgggtgcgt
      901 taattgaaga agaacagtaa agataaagca caagagcttg ggattactcc aagctctttt
      961 tgtcatttga aataaattat atctttccaa tcaaattgtt tatctgtaat ccctaatctt
     1021 tgagcaggtg tcaaaacctt tttatccgct gatttaaaag ggcgacaaaa gttgtagtaa
     1081 gttcttagaa ttgtaatcgc aaattgagca tacttcggat tgaagttagc gtaaatatag
     1141 ctcttcttat caccacgagc tgttgttaat ggtctttcaa gagatgatat ataacgtctt
     1201 atctgttgaa taaagctatt ggtcgaatgg tcattaacat ttaatagcat ctttgcgatt
     1261 tcgtttggtt ccagagcaga taaatccgtt ctacaatcaa ctgaataaaa tcctttatcc
     1321 ttagtggcta atggatgttt gatagggtta tcggcatatt tgcgatatct ttctccatct
     1381 ttactaatag cctcttcgtg gaattgatga cttgttagta attcagttag ataatgagat
     1441 gccaaagtcc gtagaaactt tgttttgaat cctctgatat ctccccaatc gagcagttct
     1501 gcttttgctt gtccgaattc ttttaagcat tgttttctgc ttttcgtctt gttaacttgg
     1561 caaataaaat gatgtgcatc gctcaacctt aattctttcg tgaatactct ataaaaggcg
     1621 tttctaatag agaagtcatc atcactaatc attcgccatt cgtcggcatt aaccatttgt
     1681 ttaattaacc aatagtgagc agttgtagta taaggagcat caacatttaa accatcgaca
     1741 aatctactcc tattagtaat tttccctaat tcgtgaagat aagcattcct attttcgcta
     1801 tcgttagcgg atggttcttg tggataataa gaccaatcta acctatcatt tttacgacta
     1861 aaagtattga gatggtcttc tttgaatttt cttgtatctt catttaattc atccattgaa
     1921 atattccaat cataggcaac atcagaacgg aacacatatc ttgataagac ctcagcagat
     1981 acaactatat acgtttgcat attaaggtct tcaaaccccg tatatttctt tgaaccttgt
     2041 cctttcttac ggacgttatt taggtagtag tgcatcttgt ccgtattaag ccacatctcg
     2101 ttaaatttct ttgtttgaag gggttgtgtc tcatatcgtt ctaaaaactc caaacaacgc
     2161 ctatacagcc actccagctt atgatagtag gttccaactc caattcctaa aatatcacaa
     2221 gtacgactga ct
//
LOCUS       DACEEZ010000251         1781 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20313_2017
            SAMN12639966-rid9879223.guided.185, whole genome shotgun sequence.
ACCESSION   DACEEZ010000251 DACEEZ010000000
VERSION     DACEEZ010000251.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639966
            Sequence Read Archive: SRR10023158
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1781)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1781)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741683.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741683.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:44:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,016
            CDSs (total)                      :: 2,962
            Genes (coding)                    :: 2,889
            CDSs (with protein)               :: 2,889
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 36 of 73
            Pseudo Genes (incomplete)         :: 29 of 73
            Pseudo Genes (internal stop)      :: 18 of 73
            Pseudo Genes (multiple problems)  :: 8 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1781
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639966-rid9879223.guided.185"
                     /isolate="E20313_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            24..416
                     /locus_tag="IWO89_003012"
     CDS             24..416
                     /locus_tag="IWO89_003012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000393259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAQ0457679.1"
                     /translation="MKLLFIDDNMVFDIDNLKGFLNDTSSFGFIAKENNKIIGFAYCY
                     TLLRPDGKTMFYLHSIGMLPNYQDKGYGSKLLSFIKEYSKEIGCSEMFLITDKGNPRA
                     CHVYEKLGGKNDYKDEIVYVYDYEKGDK"
     gene            417..>1781
                     /gene="aph(2'')-I"
                     /locus_tag="IWO89_003013"
     CDS             417..>1781
                     /gene="aph(2'')-I"
                     /locus_tag="IWO89_003013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012655884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="APH(2'')-Ia/If/Ih family aminoglycoside
                     O-phosphotransferase"
                     /protein_id="HAQ0457680.1"
                     /translation="MNIVENEICIRTLIDDDFPLMLKWLTDERVLEFYDGRDKKYTLE
                     SLKKHYTEPWEDEVFRVIIEYNNVPIGYGQIYKMYDELYTDYHYPKTDEIVYGMDQFI
                     GEPNYWSKGIGTRYIKLIFEFLKKERNANAVILDPHKNNPRAIRAYQKSGFRIIEDLP
                     EHELHEGKKEDCYLMEYRYDDNATNVKAMKYLIEHYFDNFKVDSIEIIGSGYDSVAYL
                     VNNEYIFKTKFSTNKKKGYAKEKAIYNFLNTNLETNVKIPNIEYSYISDELSILGYKE
                     IKGTFLTPEIYSTMSEEEQNLLKRDIASFLRQMHGLDYTDISECTIDNKQNVLEEYIL
                     LRETIYNDLTDIEKDYIESFMERLNATTVFEGKKCLCHNDFSCNHLLLDGNNRLTGII
                     DFGDSGIIDEYCDFIYLLEDSEEEIGTNFGEDILRMYGNIDIEKAKEYQDIVEEYYPI
                     ETIVY"
BASE COUNT          731 a          136 c          303 g          611 t
ORIGIN      
        1 tatagttgaa gaatattatc ctattgaaac tattgtttat tgatgataat atggtttttg
       61 atattgataa tttaaaagga tttcttaatg ataccagttc atttgggttt atagctaaag
      121 aaaataataa aattatagga tttgcatatt gctatacact tttaagacct gatggaaaaa
      181 caatgtttta tttacactca ataggaatgt tacctaacta tcaagacaaa ggttatggtt
      241 caaaattatt atcttttatt aaggaatatt ctaaagagat tggttgttct gaaatgtttt
      301 taataactga taaaggtaat cctagagctt gccatgtata tgaaaaatta ggtggtaaaa
      361 atgattataa agatgaaata gtatatgtat atgattatga aaaaggtgat aaataaatga
      421 atatagttga aaatgaaata tgtataagaa ctttaataga tgatgatttt cctttgatgt
      481 taaaatggtt aactgatgaa agagtattag aattttatga tggtagagat aaaaaatata
      541 cattagaatc attaaaaaaa cattatacag agccttggga agatgaagtt tttagagtaa
      601 ttattgaata taacaatgtt cctattggat atggacaaat atataaaatg tatgatgagt
      661 tatatactga ttatcattat ccaaaaactg atgagatagt ctatggtatg gatcaattta
      721 taggagagcc aaattattgg agtaaaggaa ttggtacaag atatattaaa ttgatttttg
      781 aatttttgaa aaaagaaaga aatgctaatg cagttatttt agaccctcat aaaaataatc
      841 caagagcaat aagggcatac caaaaatctg gttttagaat tattgaagat ttgccagaac
      901 atgaattaca cgagggcaaa aaagaagatt gttatttaat ggaatataga tatgatgata
      961 atgccacaaa tgttaaggca atgaaatatt taattgagca ttactttgat aatttcaaag
     1021 tagatagtat tgaaataatc ggtagtggtt atgatagtgt ggcatattta gttaataatg
     1081 aatacatttt taaaacaaaa tttagtacta ataagaaaaa aggttatgca aaagaaaaag
     1141 caatatataa ttttttaaat acaaatttag aaactaatgt aaaaattcct aatattgaat
     1201 attcgtatat tagtgatgaa ttatctatac taggttataa agaaattaaa ggaacttttt
     1261 taacaccaga aatttattct actatgtcag aagaagaaca aaatttgtta aaacgagata
     1321 ttgccagttt tttaagacaa atgcacggtt tagattatac agatattagt gaatgtacta
     1381 ttgataataa acaaaatgta ttagaagagt atatattgtt gcgtgaaact atttataatg
     1441 atttaactga tatagaaaaa gattatatag aaagttttat ggaaagacta aatgcaacaa
     1501 cagtttttga gggtaaaaag tgtttatgcc ataatgattt tagttgtaat catctattgt
     1561 tagatggcaa taatagatta actggaataa ttgattttgg agattctgga attatagatg
     1621 aatattgtga ttttatatac ttacttgaag atagtgaaga agaaatagga acaaattttg
     1681 gagaagatat attaagaatg tatggaaata tagatattga gaaagcaaaa gaatatcaag
     1741 atatagttga agaatattat cctattgaaa ctattgttta t
//
LOCUS       DACEEZ010000252         1382 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20313_2017
            SAMN12639966-rid9879223.guided.197, whole genome shotgun sequence.
ACCESSION   DACEEZ010000252 DACEEZ010000000
VERSION     DACEEZ010000252.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639966
            Sequence Read Archive: SRR10023158
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1382)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1382)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741683.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741683.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:44:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,016
            CDSs (total)                      :: 2,962
            Genes (coding)                    :: 2,889
            CDSs (with protein)               :: 2,889
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 36 of 73
            Pseudo Genes (incomplete)         :: 29 of 73
            Pseudo Genes (internal stop)      :: 18 of 73
            Pseudo Genes (multiple problems)  :: 8 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1382
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639966-rid9879223.guided.197"
                     /isolate="E20313_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            <1..1311
                     /locus_tag="IWO89_003014"
     CDS             <1..1311
                     /locus_tag="IWO89_003014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional aminoglycoside N-acetyltransferase
                     AAC(6')-Ie/aminoglycoside O-phosphotransferase
                     APH(2'')-Ia"
                     /protein_id="HAQ0457681.1"
                     /translation="ESLKKHYTEPWEDEVFRVIIEYNNVPIGYGQIYKMYDELYTDYH
                     YPKTDEIVYGMDQFIGEPNYWSKGIGTRYIKLIFEFLKKERNANAVILDPHKNNPRAI
                     RAYQKSGFRIIEDLPEHELHEGKKEDCYLMEYRYDDNATNVKAMKYLIEHYFDNFKVD
                     SIEIIGSGYDSVAYLVNNEYIFKTKFSTNKKKGYAKEKAIYNFLNTNLETNVKIPNIE
                     YSYISDELSILGYKEIKGTFLTPEIYSTMSEEEQNLLKRDIASFLRQMHGLDYTDISE
                     CTIDNKQNVLEEYILLRETIYNDLTDIEKDYIESFMERLNATTVFEGKKCLCHNDFSC
                     NHLLLDGNNRLTGIIDFGDSGIIDEYCDFIYLLEDSEEEIGTNFGEDILRMYGNIDIE
                     KAKEYQDIVEEYYPIETIVYGIKNIKQEFIENGRKEIYKRTYKD"
BASE COUNT          584 a          108 c          234 g          456 t
ORIGIN      
        1 gaatcattaa aaaaacatta tacagagcct tgggaagatg aagtttttag agtaattatt
       61 gaatataaca atgttcctat tggatatgga caaatatata aaatgtatga tgagttatat
      121 actgattatc attatccaaa aactgatgag atagtctatg gtatggatca atttatagga
      181 gagccaaatt attggagtaa aggaattggt acaagatata ttaaattgat ttttgaattt
      241 ttgaaaaaag aaagaaatgc taatgcagtt attttagacc ctcataaaaa taatccaaga
      301 gcaataaggg cataccaaaa atctggtttt agaattattg aagatttgcc agaacatgaa
      361 ttacacgagg gcaaaaaaga agattgttat ttaatggaat atagatatga tgataatgcc
      421 acaaatgtta aggcaatgaa atatttaatt gagcattact ttgataattt caaagtagat
      481 agtattgaaa taatcggtag tggttatgat agtgtggcat atttagttaa taatgaatac
      541 atttttaaaa caaaatttag tactaataag aaaaaaggtt atgcaaaaga aaaagcaata
      601 tataattttt taaatacaaa tttagaaact aatgtaaaaa ttcctaatat tgaatattcg
      661 tatattagtg atgaattatc tatactaggt tataaagaaa ttaaaggaac ttttttaaca
      721 ccagaaattt attctactat gtcagaagaa gaacaaaatt tgttaaaacg agatattgcc
      781 agttttttaa gacaaatgca cggtttagat tatacagata ttagtgaatg tactattgat
      841 aataaacaaa atgtattaga agagtatata ttgttgcgtg aaactattta taatgattta
      901 actgatatag aaaaagatta tatagaaagt tttatggaaa gactaaatgc aacaacagtt
      961 tttgagggta aaaagtgttt atgccataat gattttagtt gtaatcatct attgttagat
     1021 ggcaataata gattaactgg aataattgat tttggagatt ctggaattat agatgaatat
     1081 tgtgatttta tatacttact tgaagatagt gaagaagaaa taggaacaaa ttttggagaa
     1141 gatatattaa gaatgtatgg aaatatagat attgagaaag caaaagaata tcaagatata
     1201 gttgaagaat attatcctat tgaaactatt gtttatggaa ttaaaaatat taaacaggaa
     1261 tttatcgaaa atggtagaaa agaaatttat aaaaggactt ataaagattg attatataat
     1321 atatgaaaag ctattataaa agacggttct gttgcaaagt tttaaatcta ctatcaaata
     1381 ag
//
LOCUS       DACEEZ010000253         1365 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20313_2017
            SAMN12639966-rid9879223.guided.198, whole genome shotgun sequence.
ACCESSION   DACEEZ010000253 DACEEZ010000000
VERSION     DACEEZ010000253.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639966
            Sequence Read Archive: SRR10023158
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1365)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1365)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741683.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741683.1
            Genome Coverage       :: 63x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 00:44:33
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,016
            CDSs (total)                      :: 2,962
            Genes (coding)                    :: 2,889
            CDSs (with protein)               :: 2,889
            Genes (RNA)                       :: 54
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 36 of 73
            Pseudo Genes (incomplete)         :: 29 of 73
            Pseudo Genes (internal stop)      :: 18 of 73
            Pseudo Genes (multiple problems)  :: 8 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1365
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639966-rid9879223.guided.198"
                     /isolate="E20313_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            188..271
                     /locus_tag="IWO89_003015"
     CDS             188..271
                     /locus_tag="IWO89_003015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001814874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA methyltransferase attenuator leader
                     peptide ErmL"
                     /protein_id="HAQ0457682.1"
                     /translation="MLVFQMRYVDKTSTVLKQTKNSDYADK"
     gene            396..1133
                     /gene="erm(B)"
                     /locus_tag="IWO89_003016"
     CDS             396..1133
                     /gene="erm(B)"
                     /locus_tag="IWO89_003016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004292767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2058)-N(6))-methyltransferase
                     Erm(B)"
                     /protein_id="HAQ0457683.1"
                     /translation="MNKNIKYSQNFLTSEKVLNQIIKQLNLKETDTVYEIGTGKGHLT
                     TKLAKISKQVTSIELDSHLFNLSSEKLKLNTRVTLIHQDILQFQFPNKQRYKIVGSIP
                     YHLSTQIIKKVVFESHASDIYLIVEEGFYKRTLDIHRTLGLLLHTQVSIQQLLKLPAE
                     CFHPKPKVNSVLIKLTRHTTDVPDKYWKLYTYFVSKWVNREYRQLFTKNQFHQAMKHA
                     KVNNLSTVTYEQVLSIFNSYLLFNGRK"
BASE COUNT          510 a          215 c          219 g          421 t
ORIGIN      
        1 gggattttat tctttattta aaactttgca acagaaccgt cttgcgtatg gttaacccta
       61 aagttatgga aataagactt agaagcaaac ttaagagtgt gttgatagtg cattaaaaat
      121 tttgtataat aggaattgaa gttaaattag atgctaaaaa tttgtaatta agaaggaggg
      181 attcgtcatg ttggtattcc aaatgcgtta tgtagataaa acatctactg ttttgaaaca
      241 gactaaaaac agtgattacg cagataaata aatacgttag attaattcct accagtgact
      301 aatcttatga ctttttaaac agataactaa aattacaaac aaatcgttta acttctgtat
      361 ttgtttatag atgtaatcac ttcaggagtg attacatgaa caaaaatata aaatattctc
      421 aaaacttttt aacgagtgaa aaagtactca accaaataat aaaacaattg aatttaaaag
      481 aaaccgatac cgtttacgaa attggaacag gtaaagggca tttaacgacg aaactggcta
      541 aaataagtaa acaggtaacg tctattgaat tagacagtca tctattcaac ttatcgtcag
      601 aaaaattaaa actgaatact cgtgtcactt taattcacca agatattcta cagtttcaat
      661 tccctaacaa acagaggtat aaaattgttg ggagtattcc ttaccattta agcacacaaa
      721 ttattaaaaa agtggttttt gaaagccatg cgtctgacat ctatctgatt gttgaagaag
      781 gattctacaa gcgtaccttg gatattcacc gaacactagg gttgctcttg cacactcaag
      841 tctcgattca gcaattgctt aagctgccag cggaatgctt tcatcctaaa ccaaaagtaa
      901 acagtgtctt aataaaactt acccgccata ccacagatgt tccagataaa tattggaagc
      961 tatatacgta ctttgtttca aaatgggtca atcgagaata tcgtcaactg tttactaaaa
     1021 atcagtttca tcaagcaatg aaacacgcca aagtaaacaa tttaagtacc gttacttatg
     1081 agcaagtatt gtctattttt aatagttatc tattatttaa cgggaggaaa taattctatg
     1141 agtcgctttt gtaaatttgg aaagttacac gttactaaag ggaatgtaga taaattatta
     1201 ggtatagggc acctctaata actaccaatt aattcacctc aaaatgaaat tagaaaaaaa
     1261 cagcgaaatt tttaaatcta tttcttatcg atacaaattc ctcgtaggcg ctcgggaccc
     1321 caacatgagg aaatgttttt ataaaaaaag agtagttacc gtttt
//
